LOCUS       DAOTFI010000001        50346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_703_length_50346_cov_7.227496, whole genome shotgun sequence.
ACCESSION   DAOTFI010000001 DAOTFI010000000
VERSION     DAOTFI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 50346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50346
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_703_length_50346_cov_7.227496"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="P5558_00005"
     CDS             complement(<1..530)
                     /locus_tag="P5558_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRY22720.1"
                     /translation="MLRRTILLGCAAAAVLALGIERAEAAYPDRPITLIVPWGAGGGT
                     DATARILASLMEPALGQPVNVVNRTGGSGVVGHSAIATAEPDGYTLGIVTVEIGMMHW
                     SGLTDLTYQDYTPLALYNEDPSGVQVGIDAPWSTIDELLAYVKDNPAGTLKASGTGQG
                     GIWHLALAGMQTAGMEP"
     gene            complement(602..2044)
                     /locus_tag="P5558_00010"
     CDS             complement(602..2044)
                     /locus_tag="P5558_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRY22721.1"
                     /translation="MYGILVVALALVLLMYLAYRGLSLLILAPALAALAVVATGELPV
                     LGAYTQIFMTATGGFITSFFPLFLLGAVFGKLIEDSGSAEVLANTIIARLGQGRAILA
                     VVLACAVMTYGGVSLFVVAFATYPIADALFRRADLPRRLLPATIALGAFTFTMTALPG
                     TPAIQNAIPMPFFGTTPFAAPGLGILTALIMLGGGLAWLQYRARSLGEGYGGAATAAF
                     TPDAALREHASGEGFDLMELPVQAPPRDLPSPALAALPLVLVILLNFAFTGLVIPAMD
                     TSYLALPEFGSTDISQVRGIWSVTAALVISTLVLIALHWRRLPKLTGSVDSGANASLL
                     PIFNTASLVGFGAVIASLSAFALIRDNVVDIGGDNPLISLAIGVNLLAGMTGSASGGM
                     SIALQTLGHTYLAMAEAAGISPDLLHRVTAVATGGLDTLPHNGAVITLLAICRQTHGQ
                     AYLDIAVAGMLVPLVALAALITMGTLFGSF"
     gene            complement(2052..3146)
                     /locus_tag="P5558_00015"
     CDS             complement(2052..3146)
                     /locus_tag="P5558_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY22722.1"
                     /translation="MPVKTIDLALQGGGSHGALTWGVLDRLLADDRIRINALSGTSAG
                     AMNAVVLADGHQKAGREGSRQALRDFWKAVSDAARFSPIQRGLWDRLSGNFSLEGSPW
                     YHLFDQMTRVMTPRQLNPLGLNPLRDLLAATIDFDNVNACREVEVFVTATNVRTGRGR
                     IFRTGELSVDAVMASACLPFMFCPVEIEGEAYWDGGYIGNPALYPLVDDPVCRDIVIV
                     KINPMRRDELPETARDILNRVNELTFNSALLKELRSIHLLHQLIAAEGLESERYRDVF
                     VHIIQGSEEALHLEASSKLNAEWDYLTYLFDKGSAWAEKWLADHFDDLEVRSTIDLDD
                     LFADTFKPVDLPEDVIVESKRAGTRRERLE"
     gene            3447..4220
                     /locus_tag="P5558_00020"
     CDS             3447..4220
                     /locus_tag="P5558_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRY22723.1"
                     /translation="MNGLPVTTATGNEPILAALKRSLVERTSLLMTFVNPHAWRIEEE
                     TGEHARLLRQFDMVLPDGIGVVHLLRRLHDSSAGRFSFDATSLYPPVLATLQEAGASL
                     LVVGAKPGVAERAVARMRAAFPGVDFLAARDGYRDWAELEREILDRRPAVLLCGMGAP
                     HQERFMLRLKAAGYAGVMISCGGFLDQLAEAERYYPAWIDRHDLRWLYRVYKEPRRLW
                     RRYAVDYLPFLRFAFAALARQRLLGPGLPLPIEPSDQRN"
     gene            4386..5507
                     /locus_tag="P5558_00025"
     CDS             4386..5507
                     /locus_tag="P5558_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY22724.1"
                     /translation="MTNQGKLGIGLVGAGAIMRLSHAPTIQASERAELVAAFDLDLGR
                     AEALTAAFGGRAFGDLEAMLALPGLDAVVVATPNRHHEAAVVAAAGACKHVFCEKPLS
                     LDVAGSRRMIEACDRAGVRLQVGFNQRFWGQVEIAKALIDAGFIGRVHQMRSIYAEKA
                     SAYPAATGYRYDLAQSGGATTIDLTIHRIDLARHLLGDFSGVMAELVHSALPQQVDDN
                     VLMLTRFASGARGCLAGNRYSPNIGDGTDLYGSEGTIHLASETTGPFTSAPLAVYTEK
                     PAAELPDVLREAHYPEAWWESFKGGWITVRPPRRSPYGKQLAAFCASVLDGAPVRVTG
                     MDGLRAQEFVQAAYQSMNEGRWVDLPLADDTPFVIPAYS"
     gene            5516..6334
                     /locus_tag="P5558_00030"
     CDS             5516..6334
                     /locus_tag="P5558_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRY22725.1"
                     /translation="MRTCFHSVGLPNRPLLEVIGLAADAGYEAIEINCETLPWAPPHI
                     TPAADAGARRAVVAAAARGLPIAALGAHVGMVSADPARRDAARAFVEGCIDLAADLGA
                     PIVHILSGPIDPGQDRETAWAWFRDAVRDSTAHAAKRGIGLGVEAIAGHLFHEVDDYH
                     RLYAELPDVPFKVNFDPSHLVVQDEDPMRVVTELGARVDHVHLKDGKGTFPDFSFPPL
                     GEGAIDFPALAAGLRRAGYDGALSVEYEAQVYGYRLTEAEILKQGRAFAAALTA"
     gene            complement(6410..7186)
                     /locus_tag="P5558_00035"
     CDS             complement(6410..7186)
                     /locus_tag="P5558_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HRY22726.1"
                     /translation="MLADDIAGRPYSKTEHRRALLPLLTGRSAGAVEFKHRNISGILK
                     GFGEDWILGYKPAFNFQASLVDAVARWLARNADWRDCLPDPGAALGLEEAAQMWVGPP
                     PTLSNQPPPQELEQMLHIARKFDVAGRDERNRALGRAGEERVLAHERASLRSAGRDDL
                     VREVRWVSEEDGDGAGYDIMSYAPDGRLRLIEVKTTNGWERTPFHITRNELAVADERR
                     SAWCLLRLWNFSREPRAFELHPPLDAHVTLTATTFTAAFH"
     gene            complement(7346..10006)
                     /gene="pepN"
                     /locus_tag="P5558_00040"
     CDS             complement(7346..10006)
                     /gene="pepN"
                     /locus_tag="P5558_00040"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HRY22727.1"
                     /translation="MMSRAPERRTVRLADYAPPAFSTVRVELDFDLEPDRTTVTARQS
                     FARRAGASAPLVLDGEGLELVRIAMDGAALAPEAYRLDATCLTLPDPPERFTLEITNR
                     IAPAANTALEGLYISSGIFCTQCEAEGFRRITWFQDRPDVMTRFRVRIEAEKARYPHL
                     LSNGNPIGQGDLPKGRHWALWDDPFPKPAYLFALVAGDLALLRDHFTTRSGRTVELRI
                     YSEHELIDQCHHAMASLKKSMAWDEERYGLEYDLDLFMIVAVSDFNMGAMENKGLNIF
                     NTSATLAHPTTATDADFVQVERIIAHEYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQQ
                     FTSDMHSAAVKRIGDVTMLREGQFPEDAGPLAHPIRPAEYMEINNFYTRTVYEKGAEV
                     IRMIHTLIGEEAFRRGMDLYFARHDGEAVTCEDFVACMAEASGRDFGRFMRWYDQAGT
                     PRVVATDAWDAASGRYSLTLEQSTPPTPGQARKLPLEIPIRMGLIDRRTGDPMPLQLS
                     GENRPEGDERVLELRQAKETFTFENVTSRPLPSLLRGFSAPVELTLDLGRAELATLLA
                     RDTDTFNRWEAGQRLLLDQMQGLLGDFRAGKELALDPVILPALQAVLDDAAGDPAFAA
                     RALALPSRNVLAQSLPVIDVEGVDAVATYLRRAIGQGLAAAWVRVLEAAGESHREAPA
                     EVAPTTEAMGRRALKNTALAYLVWSGLDSAPPLAAAQYHEARNMTDRLAALKALCDVQ
                     AEPYEALLTDFHARFERSPLVINKWFALQATIEDARSVERVRRLLHHPAFTLANPNRV
                     RSLVGMFATGNLTGFHRPDGAGYRLLTETALELDRKNPQTAARLLLTLGRWRRFDPAR
                     QTLMRAELERIAATPGLSKDAYEVVTKSLG"
     gene            10132..11004
                     /locus_tag="P5558_00045"
     CDS             10132..11004
                     /locus_tag="P5558_00045"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY22728.1"
                     /translation="MAKTAFIGLGVMGFPMAGHLAAKGHEVAVYNRTGEKARAWVAKH
                     GGRAAATPRAAAEGAAVVMMCVGNDDDVRSVALGDNGVLAGLDDGAVLVDHTTASAEL
                     ARELDAAAGERGCGFVDAPVSGGQAGAENGILSIMCGGKPADFSVAEGVMQAYGRTIR
                     LIGPAGSGQLTKMVNQICIAGLVQALSEGLNFAQAAGLDTDKVLEVVSGGAAGSWQMN
                     NRWKTMAEGKFDFGFAVDWMRKDLAICLGEARRNGAALPVTAAVDQLYRLVQQQGGKR
                     FDTSSLILNLRAGK"
     gene            11123..11452
                     /gene="hspQ"
                     /locus_tag="P5558_00050"
     CDS             11123..11452
                     /gene="hspQ"
                     /locus_tag="P5558_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HRY22729.1"
                     /translation="MQARAAKFGIGQIVKHRMFPFRGVIFDVDPEFANTEEWYNAIPE
                     EIRPARDQPFYHLLAENAEAFYVAYVSEQNLLVDATGEPVNHPQIGELFDNLDAGQYT
                     PKRVYAN"
     gene            11486..12142
                     /gene="fsa"
                     /locus_tag="P5558_00055"
     CDS             11486..12142
                     /gene="fsa"
                     /locus_tag="P5558_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRY22730.1"
                     /translation="MKFFIDTADVAEIRDLAATGLVDGVTTNPSLIRKSGRDFIEVIA
                     EICEAVPGPVSAEVAATTVDGMLAEAARLVPIAPNVTIKVPLTWDGLKATKRLSQDGV
                     KVNVTLCFSAAQALLAAKAGAAFISPFVGRLDDIGQDGLALIADICQIYSQYEAITTE
                     VLVASVRTPIHVIEAAKMGADVATLPPAVLRQLVQHPLTDKGLAAFLADWEATGQRIV
                     "
     gene            12271..12717
                     /locus_tag="P5558_00060"
     CDS             12271..12717
                     /locus_tag="P5558_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22731.1"
                     /translation="MSERTACRVQWVAPRHDLGSILEAMVSDGGRLVARSAFEPETAE
                     LDELGDGELGDPAIGLGIMEVSVDPTSLARRMSAAVAAQAEAETLVVDFTGRRIRFQI
                     LPVGGDHAVIVRAPEGVQRFRRRSAGRAMGHIRRRLVDRLARPGGP"
     gene            12717..12959
                     /locus_tag="P5558_00065"
     CDS             12717..12959
                     /locus_tag="P5558_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22732.1"
                     /translation="MTKEQGTPTVEWNETAAMAIGRMRAADVASLVVMGGRGRLGRIT
                     LAELERCRSQGNWLDAVLVRDLVRLPATPVPVEQRA"
     gene            complement(12987..15953)
                     /locus_tag="P5558_00070"
     CDS             complement(12987..15953)
                     /locus_tag="P5558_00070"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRY22733.1"
                     /translation="MSETARIMPMAGPKAGGIDARIAQWAERLETHRQRVEVDPAANP
                     IELLAGELRAELQSGAIDRQTLGQLVQRLIARAFAARARRIDAYLGETDPAKNRACLV
                     EMIERLVRDPAGELLPVAQLQARLGRRLYGLVLTAHPTFSLALPLQRDLIRLAIGRAD
                     DGEALDEAARAELLERVERANHRPEPGLDLDAEHAQSVEVIHNLRNALRTVYGVVFDV
                     VHAHYPESWQEVTPCLVSLASWVGYDTDGRSDISWATTYAKRVRLQIDQFEHVVRRIG
                     ALHQLGAGAGAAAPVLELISARLALADKELRDELRVFEGFDPRDPAALERLAAMSREM
                     HRMGPSRLTDARQLEALLERALRAADDPATRRELWVLRAEIATQGLSAARTHVRINAV
                     QLHNAIRKSIDMDHAADDPVFRLSYRNAVVEMIDAAVPETVNFGTVAAENTTARRVLM
                     LVAQMLKHLDSSEPIRFLIAECETPFTLLTALYFAKRFGVERHIDISPLFETIKALDR
                     GIEVLEGALEVPAYREYLRGRGRMCIQTGFSDAGRYLGQIAASFAIERLRLGLADLLT
                     RHGLADIELVVFDTHGESIGRGAHPDSLRDRFLYYDTPECRRLLEAAGVRLTEESSFQ
                     GGDGYLFFLHEHGALAVLTRMLEHCLEPADAAADPFYAEAAYTDEFFAAIRQFNARII
                     DDPCYATLLGAFGTNMLYPTGSRALKRQHDRGSVAAVLEHPSQLRAIPHNAILQQLGI
                     LANTIGGVGQAVDKDPQIFQRLYRESARFRRLMTMVEHAFKFTDPDVLVAYLGLFSPG
                     DWLRLAQLENDPVREETLREVSGVVERMALFDRLGRVSRTLIRDYIDLARALREHRRL
                     TRDAGAEPIVVDLETRNNLNSMHALRIAVIQALMQAAVHVPDFSDRHNTTHDEVIVSL
                     MRLEVEPALELLSEVFPLTNEPRRLDFGEPASYRDTESHSYAQEHELIFRPIAAYYDV
                     IRRIGSGVLHHIGAIG"
     gene            16326..17018
                     /locus_tag="P5558_00075"
     CDS             16326..17018
                     /locus_tag="P5558_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HRY22734.1"
                     /translation="MAKYSRRAAIVGAAAGIAGALAPRSGFTQESDQQILIDRARLVV
                     EEFQSDPNFANVPIYVQNAYAVMIVPDMLQAGLIIGLEHGVGVLLARNRTTGAWSDPA
                     FYELYGGSLGLQLGGKSSDVIFTLMNATAVDKLLTSQFKLGVDASMAVGRIGAGVGAA
                     TTVRFGEDIYVFSRGRGLYGGLAADGSAVLELTEWNEAYYGAGVSAGQILRGELAQRP
                     GAQGLREALARF"
     gene            complement(17029..18237)
                     /locus_tag="P5558_00080"
     CDS             complement(17029..18237)
                     /locus_tag="P5558_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22735.1"
                     /translation="MSSDDVYRTLTARQLRAWTEDESRVMRLRTNRDLLPSGHLAARV
                     PVLVEWRSSRLDGEAPFLLLRNVNYGGSPLERTTVLHSVRMPLDGLAHVELTMVPLGP
                     GGGRGPVQHGHLRFVFEAARRPVMLDLDGSPVGADARLDDLVLSWEPWRFRDDRFSFV
                     KALGDDFDLCLRAYAGAQRYLEDVLDGHEWWSYRLRLPGGAEGALELLRVALAMGDGA
                     ARHTLARLLDQVPADWLGAAPAGAGVEADGGPGDDWEALRRRIETPGPLAFHGPELAP
                     EELTYQTLVRSCAAMARYTVLLTARRLVDRGLADGVDPAKLPEPALGVTEPWMQAIPK
                     ADLRGLFMRAPSALRYIARNPRVLPNLIPGELDAAGLLVRKGRKPWLLKYGAKGLRPY
                     SSQGARRFEP"
     gene            complement(18335..18904)
                     /locus_tag="P5558_00085"
     CDS             complement(18335..18904)
                     /locus_tag="P5558_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRY22736.1"
                     /translation="MQKPLEIRFQQMDPSPAVEARIREKVEALERVAPRMTSCRVVVE
                     KEDRHQTKGNLYRVRLDIGMPGKEIAVSRAGPKDHAHEDVYVAVRDAFAAAQRQLRER
                     SRSVAGKVKQHEAPLTGRVVRLFPDEGYGFVETPAGDVYFHRNSVADGGYDALAVGAE
                     VRLEVAENESPEGWQATTVQATGRVLAEE"
     gene            19552..19824
                     /locus_tag="P5558_00090"
     CDS             19552..19824
                     /locus_tag="P5558_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22737.1"
                     /translation="MAHACPTSFGLLGLALATVAAAQVMAQESAVDVVAVAVRDAGHA
                     CTDPRDAVRDAARSAPNEAAWTIRCDEGAYSVRFMGDTGAKVEPIP"
     gene            complement(19856..20113)
                     /locus_tag="P5558_00095"
     CDS             complement(19856..20113)
                     /locus_tag="P5558_00095"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HRY22738.1"
                     /translation="MSRLLVLGLGYGFLVLGVLGLFLPVLQGVLFLVVGLVILSRHAP
                     WAERLLARFKARFPRLGSVFTTGENLAERWIHKLGRRVRGS"
     gene            complement(20110..22182)
                     /gene="ligA"
                     /locus_tag="P5558_00100"
     CDS             complement(20110..22182)
                     /gene="ligA"
                     /locus_tag="P5558_00100"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRY22739.1"
                     /translation="MEALDEKAAAAELARLATVIARHDRLYHQEDAPAISDADYDALV
                     RRNRAIEARFPALVRPDSPSLRVGAAPAEAFGKVVHPVPMLSLDNAMEDAAVVEFLAR
                     IRRFLNLAADAPVELLAEPKIDGLSCALRYEAGLLVTGATRGDGTTGEDITANVRTIR
                     DIPQRLDTGDPPPVLEVRGEIFMELEEFRKLNERRAKEELPVFANPRNAAAGSVRQLD
                     SAITATRRLRFFAYGWGAAEPPVTGSYHGFLERLAGMGLRVNPLNRICRSAAELLAYH
                     AEIGERRAGLGYDIDGVVYKVDDLRLQQRLGFVGRAPRWAIAHKFPAQQAVTVVDEIV
                     IQVGRTGALTPVARLAPITVGGVVVGRATLHNQDYIEEKDIRVGDTVVIQRAGDVIPQ
                     VVEVVTDRRPKGAAPFVFPETCPECGSHAVRPPGEAVRRCTGGLICPAQVEARLEHAV
                     GRDAFDIEGLGRRQVPQLIEAGLLRSPADIFRLAGDPVKQEALAELEGWGKKKIENLV
                     AAIEARRAIPLDRFILALGIRFIGEANARLLARHYGTLEAWRQAMLDVAGGDEDARAE
                     LADIDGIGPRLVEAIAEFFQEPHNIAALDDLAGEVMVEPVAAPAGGGSPFSGKTLVFT
                     GSLEQMTRAEAKALAEQLGAKVAGSVSKKTDYVVLGADAGSKARKAVELGVETLDEAA
                     WLAMAGVT"
     gene            complement(22203..23879)
                     /gene="recN"
                     /locus_tag="P5558_00105"
     CDS             complement(22203..23879)
                     /gene="recN"
                     /locus_tag="P5558_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRY22740.1"
                     /translation="MLLSLAIRDIVLIERLDLELGPGLTVLTGETGAGKSIILDALGL
                     ALGQRADRVLLRSGAAQGSVVAEFRPPAGHAAVALLAGQELAGEAGELVVRRTLAADG
                     RSRAFVNDVPVSGAILRELGDALVEVHGQMDQRGLLDSRSHRAVLDAFAGHGDQLAAT
                     RAAHAAWRGLEAEILESRARVEQARREEDYLRHRLLELEALAPEAGEEERLAEERSRL
                     LHKGKLVTALEEALALVAGPEAALARLGSAERRLARAPEVATSLVEPALAALGRALIE
                     AGEAEAQIEEALREVAAEERSLEAVEERLFALRDAARKHRTAVDDLPALLERTAAELG
                     ALDAGAEALEGLERAARAARGRYLEVARALRRSREAAAGVLEKLVAAELPPLKLDKAR
                     LAIRLEPLDEEAAGPDGLDRVAFLVATNPGQPLGPIAKIASGGELSRLMLALKVVLAR
                     DAASGTLIFDEVDAGIGGATADAVGERLARLARERQVLVVTHAPQVAARADHHLKVEK
                     LTDMEGARVEVRPLDAAERRDEIARMLAGAEITAAARAAATSLLDAGGVR"
     gene            complement(23923..24786)
                     /locus_tag="P5558_00110"
     CDS             complement(23923..24786)
                     /locus_tag="P5558_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRY22741.1"
                     /translation="MFASITGLKSAGHASAGRRLPDARRLVLALLVALAPLLAACGGN
                     SGADDYVERTAEELFSEGEQQMAAGNHEDAARAFEEVERQHPYSQLAVRGQVLAGYAY
                     YEGQLYEEAIAALRTFIELHPGNPDVPYAHYLIGMSYYERISDVGRDQEMTQEALESF
                     GELVRRYPDSEYSRDAELKMDLAFDHLAGKEMEIGRYYQKRGEFVAAINRFRAVVENY
                     DTTTHVPEALHRLTESYLALGVFQEAQEAAAVLGYNYPDSVWYQRSYALMQQEEAGAP
                     ASEGSWLTGLF"
     gene            complement(24930..25577)
                     /locus_tag="P5558_00115"
     CDS             complement(24930..25577)
                     /locus_tag="P5558_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRY22742.1"
                     /translation="MSLSVLVTNTKGGCGKTTIATTLATAFAVAGFKTALADVDRQRS
                     SLGWLATRPATAAPITGLDWRKAADSKPAGTQRLVIDVPAGLRLGHVQDLLKSADVVI
                     VPVLPSVFDERSTSRFLEKLESIKPIARGRKRVLVVANRLRPRQKAAQHLLEHLAEAG
                     LEPTARLAERTLYGELAMGGLGLFDLDQRRDQRRAAAGREDWLPLLAAIERAAAR"
     gene            complement(25574..27208)
                     /locus_tag="P5558_00120"
     CDS             complement(25574..27208)
                     /locus_tag="P5558_00120"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HRY22743.1"
                     /translation="MRAPSCDHEIELKLMVDPADLARLAARLGASPFAFSAPEVLPLE
                     SRYFDTPDLRLAARRVSLRVRRSGERHVQTIKTASVGGAAHTVRGEWECTVAAMAPEL
                     DRIGDPAALEALGLVLPEELAPVFTTRVERRLMLVEQKLRDGSKAIIEVAFDRGTIEA
                     DALATGPGAGKAGKKARVEQIAEVELELKAGAPEGLYLLLETLSDWATLRISTTPKAQ
                     RGYLLATGRPPAWQRAEPPALDPAIPAGTAMAAVLASGLQQLLQNQAAAQDGRDPEGV
                     HQLRIGLRRIRSGLALFASLLPVDERTAWDERLKAVIAATGTPRELDVLVTETLAEIK
                     AGLPDDPALAVLTATAEAARRRAYATLRTYLQSRELAGLVLDLALWTALERWRDDTTE
                     ECAAAQSTPVIELAVALLERRHKRVRRRGRHFARLGDEERHEVRLALKKLRYGVEFLS
                     GLFPEKPAKRYAKAAARLQDRLGRLNDQADTRRLLEALAGSAGPGTDRPALERGIGLV
                     IGWQAACVGAERRLAVDDWHDFTAQELFWQELDEDR"
     gene            27395..28237
                     /locus_tag="P5558_00125"
     CDS             27395..28237
                     /locus_tag="P5558_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY22744.1"
                     /translation="MSLKDLLVHIDDKPSSDKRLDAAVALALACDAHLAGLMLVAEPY
                     VPAAIGVSIPAEVLREQREAAVQRCDAVLAKAKARADQAGCRLEVRREVVMVDDFGTA
                     FARQARHADLSIVGQADPNEGDVDAELVVEASFLDSGRPALVIPYIGARQIPPKRVLI
                     AWDGSREAARAVGDAMPLMRKAESVLVLVVDPAKLKGRIGEQPGADLGEHLARHGLKV
                     EVRTAASGGQAVGDVIIGQASDAGIDLVVMGAYGHSRLRELVLGGATQSLLDHMPIPV
                     LMAH"
     gene            complement(28268..30109)
                     /locus_tag="P5558_00130"
     CDS             complement(28268..30109)
                     /locus_tag="P5558_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22745.1"
                     /translation="MLGALCIALIAALGYGYRLYQQQGARLASLERSGGPVVAEAVGR
                     GGVAPAAGALDGTAGASMEALADEVARLESELAAVTGRSTATSEQAARELAAVQLKLA
                     HAEEARADAETRLAAADSRITALETELGVGGAIEAMIDSAGQPMVAPEAVTPDQLTQL
                     GMELRLVERERDAARLQLDQALQRMATLEVAGPVRAAGPAATGDPASAARLDQLEAQN
                     RDLAAEKAVLEATLERGRAQQAEALATLETRAGDLAAELTSAEARRADLEAGNARLAR
                     ELASAVAERAGLTQQLAAATAEADALRAENLQTESRAQVLVEERDALQAELGAAAAAV
                     AERDRLASELGRGQELLSELADRLTRLEAQTAEAETRTTALVAERDALAAELATARAA
                     QAAEVDVAAAADAAVASELAAVRAERDALRADVAAARMAGEAATAQLAELRTAAAAGA
                     TAAERAGTLAAELAEARDARALAGREAAGLRAERDSMARASAAALDAATAAHDAELAE
                     LAARNAELERRLEAQTTSSGAITPEAYAAANQRIAGLEEELRTMARLAAELRPTDGVV
                     SDTAELMAELERLKGVEEELRETRARLDALQTVHLDVAPAPPPPAPR"
     gene            complement(30427..31389)
                     /locus_tag="P5558_00135"
     CDS             complement(30427..31389)
                     /locus_tag="P5558_00135"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRY22746.1"
                     /translation="MSKNRDDRMVVLASEPAPAVQRTLRHPIGCVGVGRHSGARVALT
                     LHPAPAGSGISFRRTDRAGALPVPAAARHLVPSEHAFVLVGEDGSRIASVEHVLAALA
                     LTGIHNALIELNGPEVPAMDGSAQPFVFLIECAGVASQDVLCASRPCIVDAVEVRRGT
                     AWARLAPAEQPSLVSTVTLPELGSLTHRVALRPEALRHELAAARHSASLADIVAGQER
                     GLWRGVSARNTVLFDGPTPTNEEGLRCADEPARHGNLLALAALGLLGGQPGGLPAVEL
                     VTEEAGCGLLAELLRRALALPARPAAEVPAEALPVREPLGARTS"
     gene            complement(31625..33196)
                     /gene="ftsZ"
                     /locus_tag="P5558_00140"
     CDS             complement(31625..33196)
                     /gene="ftsZ"
                     /locus_tag="P5558_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRY22747.1"
                     /translation="MTISLSMPVTHELKPRITVVGVGGAGGNAVNNMITSHLEGVEFV
                     ACNTDAQALANCLTDRKIQLGHAITHGLGAGARPEVGRASAEESLDEILDLLQGSNMV
                     FITAGMGGGTGTGAAPVIARAVRDMGILTVGVVTKPFHFEGAHRMRLAEGGLTELQQF
                     VDTLIVIPNQNLFRLANERTTFADAFQMADQVLHMGVRGVTDLMVMPGMINLDFADIR
                     TVMSEMGKAMMGTGEAEGERRAIEAAEAAISNPLLDEVSMRGARGVLINITGGADITL
                     FEIDAAANRIREEVDPEANIIFGSTFDESLQGALRVSVVATGIETPAERAKQAAAESS
                     ATHEAQGQGQAGGWFPQAPYRPQPYPAVQKPAQRAPEANPTLQLRAAAEGRREAAPAG
                     DSQYPSFVPPPAQGAPVRVEQPNDGYDAVARRVGPRQEDRSIGRGIFERVKGIAFPRG
                     FTGHGPETGNGQPLAPSPFRREPALAPAHGPAEPAYKAPAPAEGAQERGRNATPSQDE
                     LGDFPAFLRKERVES"
     gene            complement(33325..34629)
                     /gene="ftsA"
                     /locus_tag="P5558_00145"
     CDS             complement(33325..34629)
                     /gene="ftsA"
                     /locus_tag="P5558_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRY22748.1"
                     /translation="MNAKAEMAWQRLLQSQRRTARNAPFGLLDVGSSKLCCYVVRTQS
                     SGGFQLLGRGYQSADGFQAGEVSDAEAAETSMLAVLHEAEEAAGETLREIAVTWSGGA
                     PRAGLVSIERDLGGRVIDDDDLDRALESVRATMVGSDREILHILPVEMRLDGGRPLAD
                     PRGLAGQVLEFTGLVVSVAKPALRNLTACLERCHLDIMEVANGAYAAGVGCLTLDEMR
                     RGVLLVELGGGSTSHALFQGGALCHLGQVPYGGEHVTRDLAYGLKTSRVFAERLKNLY
                     GGVQWRACDDNVRVEVPLIGDQLEQPTGEVPRTRLTQIIRARVEETLGLVQERLKDDW
                     ELVQSRPPRSMVLTGGAAQMEGIVELAEELFGLPARIGRPGAVRGPAGVEDQPCCSAA
                     AGGLVLVTGRDGGLGWKQSTEVPALTNGLARLGHWLKQNFVS"
     gene            complement(34626..35480)
                     /locus_tag="P5558_00150"
     CDS             complement(34626..35480)
                     /locus_tag="P5558_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRY22749.1"
                     /translation="MRQMRQLAYRTRHVLRQPPKPRWHEPLINGLVAVGCAAVLGLSL
                     HLARASGLTDRARFALNDQLVAVTDAVGMQVREIFTEGRQRTTAAEIVQILEPYYGQN
                     ILSVDIAALQKRLESLPWVQNATVTRQLPDQLRTRLVEHRPIALWQTANGPALVSVEG
                     DIIQVRSLSPYRHLPILSGAGAPAGASELMRLVASVPSLGERVSGAQRVDGRRWNVFV
                     DGRIEVRLPERGAAAAWARLARLEARSALLARAVEAVDLRLPDRVVVRLDEAILQSAA
                     HATRGQQT"
     gene            complement(35468..36388)
                     /locus_tag="P5558_00155"
     CDS             complement(35468..36388)
                     /locus_tag="P5558_00155"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRY22750.1"
                     /translation="MNRHVAVLMGGLSAEREVSLVSGENCAAALEGQGYRVTRVDVGR
                     DLPMVLAELKPDVVFNALHGRMGEDGRVQGVLDLMGIPYTHSGVLASALAMDKPMAMR
                     LFRSAGLVCPEGFEIEFEDLQDGPPPMEPPFVVKPAAEGSSVDVVIAMKGDIGGLVDR
                     EDIAPDTRFLVERYVPGHELTCAVLGEEALTVTEIRPKKKGGFYDYRAKYTEGVADHI
                     VPAPLPRPVLDKVCDWSLRAHWVLGCRGVSRADFRFDPSKGMDGLVLLEVNTQPGMTP
                     LSLVPEQARHRGIGFGELVVRLIERARCDR"
     gene            complement(36385..37323)
                     /gene="murB"
                     /locus_tag="P5558_00160"
     CDS             complement(36385..37323)
                     /gene="murB"
                     /locus_tag="P5558_00160"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRY22751.1"
                     /translation="MTARREAVRGTMREQVTLAPLTWFRVGGPAARLFQPADADDLAS
                     FLAATPAEVPVLAMGVASNMLIRDGGIGGVVVRFGGPLARIEIDGTTLIAGAGALDQK
                     AAQAAERAGLAGFEFMIGIPGTIGGAVRMNAGAFGGETRDRLLWAEALDRQGRLHRLT
                     NAELGFAYRKSALPEDWIVVRAAFGLQPGEPGAIGERMAAIRAEREAVQPIRVATGGS
                     TFKNPPGQRAWQLVDAAGCRGLRRGAAMVSEKHCNFLINTGGATAAELEALGEIVRDR
                     VAAQSGIRLEWEIKRLGEASPDVVPAEPRQTVGEHR"
     gene            complement(37320..38729)
                     /gene="murC"
                     /locus_tag="P5558_00165"
     CDS             complement(37320..38729)
                     /gene="murC"
                     /locus_tag="P5558_00165"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRY22752.1"
                     /translation="MRIQPLDCGVIHFVGIGGIGMSGIADILHGMGYAVQGSDVAESA
                     NVQRLRDMGMAVAIGHGAENLGDARVLVVSSAIKADNPELVEARRRRLPIVRRADMLG
                     EIMRLHPSVAVAGTHGKTTTTAIVAALFDAAGLEPTVINGGIINAYGTNARLGQGEWM
                     VVEADESDGSFLRLPATIGIVTNIDPEHMEFWGSFDAVRDGFRDFIERLPFYGLAVLC
                     IDHPEVQALIGRIGDRQIRTYGFSAQADIRATEPISNAKGSTFDVFVRDPATGEQRRF
                     VDLHLALPGYHNVQNALAAIAIADQLGFGEDVVRKALTGLGGVKRRFTVTGEVDGITI
                     VDDYGHHPVEIRAVLKTARQATGGRVIAVVQPHRYSRLEELFEAFCTCFANADSVLVA
                     PVYAAGETPRPGVDHAALVAGLIGHGQRDVAAIDGPDELPARIAELAQPGDLVVCLGA
                     GSITQWANALPERLRELRA"
     gene            complement(38744..39841)
                     /gene="murG"
                     /locus_tag="P5558_00170"
     CDS             complement(38744..39841)
                     /gene="murG"
                     /locus_tag="P5558_00170"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRY22753.1"
                     /translation="MRGPILVTAGGTGGHMFPALALARELRGRGEPVAMVTDRRGARF
                     MPEDLEAHLVRAGSPTGSLPRRLAGICALVAGIFDAWRVARRLKPRAAASFGGYASVP
                     AAIAARLAGRPLLLHEQNAVFGRANRLLARFAEVIALSFEATSAVPERGRARLVVTGN
                     PVRPGFEADPAGYRAPGPNEPIRILVIGGSQGARIFSDVVPAAVALLPAELSRRLRIE
                     QQCRPEDLARVADAYKAHGIAAEVAGFFTDMPAHLARAHLVISRAGASSLTEIMAMGR
                     PSILVPYAAAADDHQRANAEALARAGGAVRMLQASFTPVALAAELTALAAAPERLEAM
                     ATAALATFRRDAAGRLADAVLALVPATGESP"
     gene            complement(39838..40950)
                     /locus_tag="P5558_00175"
     CDS             complement(39838..40950)
                     /locus_tag="P5558_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRY22754.1"
                     /translation="MNAFARTDRSLLGQWWWTVDRFLLIGIGLLILFGIIMVLASSPP
                     IARNLKLDQMHFVIKHFTVLVPTALGLFGVSLLAPRGVLRLAVGVLALFAVLTLATLW
                     LAPEIKGAHRWLFIGGQQLQPSEFMKPALAVVTAWLLASRPGLGGLPAALLLVGPTVA
                     LLLLQPDLGMATVVVAFFSIQLFVAGLGWLPIIILGGAGVAGLWGAYQLLPHVRDRVD
                     GFLDPTAEVYQVERALSAVQSGGLFGRGPGEGVVKFRLPEAHSDFVFATVAEEFGIII
                     CLVLVAVFCALVLRALWRTQLNPNRFVQLAATGLVAQFGLQALINMAVNLNLVPTKGM
                     TLPFISYGGSSLLAVALTMGMLLALTRRGARLEPVP"
     gene            complement(40947..42311)
                     /gene="murD"
                     /locus_tag="P5558_00180"
     CDS             complement(40947..42311)
                     /gene="murD"
                     /locus_tag="P5558_00180"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRY22755.1"
                     /translation="MGGKGRIGILGMARSGRSVLRDALARGIDCLVYDDRPESVAAAV
                     AEGGRAGKVGDVAGLDALVVSPGVPLYFPAPHAVIAAARAAGVPLTSDIDLFAERLAG
                     RTVVGITGTNGKSTTTALAHHLLVAAGRDAELGGNIGRPVLDLELGGSGRVFVLELSS
                     YQLDLALKLACQVAVLLNLTPDHLDRHGTFEAYVAAKRRLFELQPEGGVAVVGVDDAP
                     SRALADALAAAGRKVIRISALGPVPGGVWAEGTLLVDGTGETPRTVADLAGIESLEGR
                     HNRQNAAAAWAVLRALGVAAEAAVGGFASFRSLPHRMQTVARAGAVRFVDDSKATNPE
                     AAIPSLSSFAEIFWIAGGKPKPGGFAALLPHLAAVRGAWLIGEAAGEIAATLPAAMPR
                     HLAGRLDVAVPEAFAAARTSNAPEPVVLLAPACASFDQFTSYEARGAAFATLARELAE
                     GVAA"
     gene            complement(42317..43402)
                     /gene="mraY"
                     /locus_tag="P5558_00185"
     CDS             complement(42317..43402)
                     /gene="mraY"
                     /locus_tag="P5558_00185"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRY22756.1"
                     /translation="MLYLLLPFADEISVLNVFRYITFRSGGAAMTALLISFILGPPVI
                     RWLKQKQREGQPIREDGPESHLLTKKGTPTMGGMLILIAIAVSTLLWADLANGYVWVV
                     LVVTLGFGGIGFADDLMKLTRRSSAGISGRMKLFLQGTIALVAAVVLVRLTPEPLDTS
                     LALPFFKDLLVPLGLFFPLFGMLVIVGASNAVNLTDGLDGLAIVPVMIAAGCFALIGY
                     LVGNAIFSGYLGIPFVPGTGELAVFCAAMVGASLGFLWFNAPPAMVFMGDTGSLATGG
                     ALGTIAVATKHELVLAVIGGLFVLEAVSVIVQVVSFKLTGRRVFRMAPLHHHFEKKGW
                     LEPTIVIRFWIIATILAMIGLSTLKLR"
     gene            complement(43402..44829)
                     /gene="murF"
                     /locus_tag="P5558_00190"
     CDS             complement(43402..44829)
                     /gene="murF"
                     /locus_tag="P5558_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRY22757.1"
                     /translation="MSMVTLKPLWEAVDAAAATGGRSRRDWHAAGVSIDSRSVGEGDL
                     FVALAGPSRDGHGFVAHALRQGAAAAVVTRRPEDVDEDAPLLVVDDTQSALEGLGRFG
                     RARSRARVVGITGSVGKTGTKEALRHVLGGFGRVHASAASHNNHWGVPLTLANLPPEA
                     DFAVAELGMNHAGEIRALTAMVRPHVAVITAIAPAHLAFFEDTAAIARAKAEIFEGLE
                     PGGTAILNADAPHADLLVEAARARAARVVLFGAVGEADVRLVALDLEAGGSRLTVALG
                     GRRLELALAAPGRHWAMNSLAVLAVADCLGLDLPHAAARLGEVRPLAGRGGQRLLPWQ
                     GGRIRLLDESYNANPASVTAALDVLGSQAGRRIAVLGDMLELGAGAGLLHAGLAHAVL
                     AADASKVFLAGPLMQELFDALPASRRGAWAADSEALAPLVVAALEPGDVVLVKGSLGS
                     AMRRVVEALDRTANPSATPVEGQGR"
     gene            complement(44826..46337)
                     /locus_tag="P5558_00195"
     CDS             complement(44826..46337)
                     /locus_tag="P5558_00195"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRY22758.1"
                     /translation="MRLPALLAEEARREAARGRKPAAAGRLLNLAPVTITGIAFDSRK
                     VEPGNLFAALSGTVADGHIFIPEAVQRGAVAVLGTTAAEAYAQLVPVIIDPDPRRRLA
                     TLAAAFYPGQPAFTAGVTGTNGKTSIASFTRQIWDRLGIRAASLGTLGLEGRGRWPSV
                     SLTTPDAVTLHRVAAELVRGGVGHLVVEASSHGLDQQRLAGLDIHAAAFSNMSRDHFD
                     YHGSYHDYFMAKRRLFTECLVPGGTAVLNADSPEFPTLVKICNKRGCPVVAFGEAGED
                     LKLLGRKPELTGQVLEIAVHGRTHRLRTRLVGAFQAHNVLAALGLAVAGGAPLEQAIL
                     ALAELRGAPGRMQLVVRHPSGAPAFVDYAHTPDALAKALDALRPHTKGRLHVVFGCGG
                     NRDKGKRPLMGEVAVERADRVWVTDDNPRNEDPAAIRRQILEPLAGRAGKVTEIGDRA
                     AAIRAAFAALEPGDVLIVAGKGHEQGQIVGEETLPFDDATELRTALAGLESAA"
     gene            complement(46312..48033)
                     /locus_tag="P5558_00200"
     CDS             complement(46312..48033)
                     /locus_tag="P5558_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRY22759.1"
                     /translation="MSRSAGGANALTTARLRVLALCFVLAFVSIGIRLVDMVGGNRAE
                     AAFVPPGGDDAPDPRRADITDRNGALLATNIATLSVVGDPMRMNDPDAVATALARVLD
                     GVDATELRARFARGGRFAWIKRQISPIEQAAVLDLGLPGVEFRLGERRVYPRGSEVSH
                     VLGFVDIDNQGLAGIEHGLQEALVGGSELGKPPVRLSIDLRIQRIAHAATSAGLQRFR
                     AEGACTILLDMATREVVALVSLPDFDPNRPEQASPEQRRNRCTSSVYELGSLFKVVSA
                     GMALESGAVSLHDRYDASEPLQIGRHRVRDYHGHNRWLTVPEVFAFSSNIGTARMTFA
                     AGGGEAQRAFLKALGLLDRPVLEIPETTAPLVPERWPDIVTATVSYGHGVAVSPLQFV
                     EAVATLAGDGRRVPATLLERPADAAIDGPTVVSAATARDVRWLMWLTVDGGTATHAAV
                     PGYLIGGKTGTADKPTAGRRGYDGQAVLASLVGVFPIDAPRFAVLTMLDEPKGDAKTY
                     GYRTGGWTAAPVVARIIAEAGPLLGVPRAQPESELALRDRLQVVPVMNGRTRRMEDGF
                     AAASLTR"
     gene            complement(48030..48440)
                     /locus_tag="P5558_00205"
     CDS             complement(48030..48440)
                     /locus_tag="P5558_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22760.1"
                     /translation="MIPRYVPVLLVLILVAATGLFRVKYAVVSAESEIVRIEREIEAE
                     RWRLKTLEADWAYLVRADRLTAQARALDMQPLRLSRVVQAEQIGNYRNLELAREARSA
                     LLPDGEAIEFRVKPVATFDLGVLGAGGDRLGAEW"
     gene            complement(48437..49456)
                     /gene="rsmH"
                     /locus_tag="P5558_00210"
     CDS             complement(48437..49456)
                     /gene="rsmH"
                     /locus_tag="P5558_00210"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRY22761.1"
                     /translation="MESGGSAVAHEPVLLDAMLETLAPAAGETIVDATFGGGGYSRAI
                     LAAASCRVIGIDRDPAAVARGRALAAAEPRFTMLEGTFGELPRLLGAAGTGLVEGPVD
                     GIVADLGVSSFQLDEAARGFSFQADGPLDMRMGGTGETAAELLARLDEAELARLLREL
                     GDEPEAGRVARAIVRRLAERPITTTGELVSVVTVAKRQKKPGRSPATQVFQALRIAVN
                     DELGELDRLLEAAPDCLAPGGRLVLVAFHSLEDRRIKRFIDQAAGRVARPSRHLPLTT
                     EATRARLRWLERKVVRPGEAEVQTNPRARSARLRAAVKLAEEGEAVTHGASGGSMVET
                     WRAAA"
     gene            complement(50000..>50346)
                     /gene="rnpB"
                     /locus_tag="P5558_00215"
     ncRNA           complement(50000..>50346)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5558_00215"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         7222 a        17999 c        18041 g         7084 t
ORIGIN      
        1 ggctccatgc cggcggtctg catgccggcg agcgccaggt gccagatgcc gccctggccg
       61 gtgcccgacg ccttgagcgt gccggcgggg ttgtccttca cataggccag aagctcgtcg
      121 atcgtgctcc agggcgcatc gatgcccacc tgcacgcccg agggatcctc gttgtagagg
      181 gcgagcggcg tgtagtcctg ataggtcagg tcggtgaggc cggaccagtg catcatgccg
      241 atctcgaccg tgacgatgcc gagcgtgtag ccgtcgggct cggccgtggc gatggcgctg
      301 tggccgacca cgccggagcc gcccgtgcgg ttgacgacgt tcaccggctg gccgagcgcg
      361 ggctccatca gggatgccag gatgcgcgcc gtggcgtcgg tgccgccgcc ggcaccccag
      421 ggcacgatga gcgtgatcgg ccggtccgga taggccgcct cggcccgctc gatacccagc
      481 gccaggaccg cggcagcagc acagcccaga agaatcgtcc gtcgcagcat ggctcgaagc
      541 ccccctgttt cttgcgatgc gatgggggga cgctagccga tccttggccg ttcggccagc
      601 atcagaagct gccgaagagc gtgcccatgg tgatcagcgc ggcgagggcg acgagcggca
      661 ccagcatgcc ggccacggcg atgtcgagat aggcctggcc atgggtctgc cggcagatgg
      721 cgagcagcgt gatgacggcg ccgttgtgcg gcagcgtgtc gagcccgccg gtcgccacgg
      781 cggtgacgcg atgcaacagg tcgggcgaga tgcccgcggc ctcggccatg gcgaggtagg
      841 tgtggccgag cgtctgcagg gcgatgctca tgccgcccga ggccgagccg gtcatgccgg
      901 cgagcaggtt gacgccgatg gcgagcgaga tcagcgggtt gtcgccgccg atgtcgacca
      961 cgttgtcgcg gatcagggcg aaggcggaga gcgaggcgat gacggcgccg aagccgacga
     1021 ggctcgcggt gttgaagatc ggcaggagcg aggcgttcgc gccgctgtcg accgagcccg
     1081 tcagcttcgg caggcgccgc cagtgcaggg cgatcaggac gagggtcgag atgacgaggg
     1141 ccgcggtcac cgaccagatg ccgcgcacct ggctgatgtc ggtcgagccg aactccggca
     1201 aggccaggta cgacgtgtcc atggccggga tgacgagccc ggtgaaggcg aagttgagca
     1261 ggatgacgag gaccagcggc agggccgcca gggccggcga cggcaggtcg cggggcggcg
     1321 cctggaccgg cagctccatg aggtcgaagc cttcgcccga ggcgtgctcg cgcagcgccg
     1381 cgtcgggcgt gaaggcagcg gttgcggctc ccccgtagcc ctcgcccagg ctccgcgccc
     1441 ggtactgcag ccaggcgagc ccgccgccca gcatgatcag ggcggtgagg atgccgaggc
     1501 cgggtgccgc gaagggcgtc gtgccgaaga acggcatggg gatggcgttc tggatcgccg
     1561 gcgtgcccgg cagggccgtc atggtgaagg tgaacgcacc cagggcgatg gtcgcgggca
     1621 ggaggcggcg cggcaggtcg gcccggcgga agagggcgtc ggcgatcggg taggtggcga
     1681 aggcgacgac gaagagcgag acgccgccat aggtcatcac cgcgcaggcg agcacgacgg
     1741 cgaggatcgc ccggccctgg ccgagccggg cgatgatcgt gttggccagc acctcggcgc
     1801 tgccggaatc ctcgatcagc ttgccgaaca cggcacccag gaggaagagc gggaagaagc
     1861 tggtgatgaa gccgcccgtg gccgtcatga agatctgcgt gtaggcgccg agcaccggca
     1921 gctcgcccgt ggcgacgacc gcgagggcgg ccagcgccgg cgccaggatg agcaggctga
     1981 ggccgcgata ggccaggtac atgagcagca ccagggccag ggcgacgacc aggattccgt
     2041 acatggccgg tctactccag gcgctcgcgg cgggtgccgg cgcgcttcga ttcgacgatc
     2101 acgtcctccg gcaggtcgac cggcttgaag gtgtcggcga agagatcgtc gaggtcgatg
     2161 gtggagcgga cctccaggtc gtcgaaatgg tcggcgagcc acttctcggc ccaggcgctg
     2221 cccttgtcga agagataggt caggtagtcc cactcggcgt tgagcttgct cgacgcctcg
     2281 agatgcaggg cctcctctga accctggatg atgtgcacga agacgtcgcg gtagcgctcg
     2341 ctctccagcc cttccgccgc gatgagctga tggaggaggt ggatggagcg cagctccttg
     2401 agcagggccg agttgaaggt cagctcgttg acccggttca ggatgtcgcg cgccgtctcc
     2461 ggcagctcgt cgcgacgcat cgggttgatc ttgacgatca cgatgtcccg gcagaccggg
     2521 tcgtcgacca gggggtagag cgccgggttg ccgatatagc cgccgtccca ataggcctcg
     2581 ccctcgatct ccaccgggca gaacatgaag ggcaggcagg ccgaggccat caccgcgtcg
     2641 accgagagtt cgcccgtgcg gaagatccgc ccgcgaccgg tgcgcacgtt ggtggcggtg
     2701 acgaagacct ccacctcgcg gcaggcattg acgttgtcga agtcgatcgt cgcagccagc
     2761 aggtcgcgca gcgggttgag gccgagcggg ttgagctggc ggggtgtcat gacgcgcgtc
     2821 atctggtcga acagatgata ccagggtgag ccctcgaggc tgaaattgcc ggagagccgg
     2881 tcccagaggc cgcgctggat gggcgagaac cgggcggcgt cgctgaccgc cttccagaaa
     2941 tcgcggagcg cctgcctcga cccttcgcgg ccggccttct gatggccgtc ggcgaggacc
     3001 accgcgttca tggccccggc gctggtgccg ctcagcgcgt tgatcctgat ccggtcgtcg
     3061 gcgaggaggc ggtcgagcac gccccaggtc agggcaccgt gcgagccgcc gccctgcagc
     3121 gccagatcga tcgtcttcac cggcatcgcg caccctcctg cccagccgcg ccagcccggg
     3181 cggcaggccc cctaattgtg cattgcagcg tgaatggcaa cggtgccgcg ccgcgacaag
     3241 tgagcacgcc acggctccat cgagcgcgat cggacaggtt ttcgccatcc ttcattcggg
     3301 cggcatcagt aatactgtgc gcctgttaac catgaccctt aacttctgct tgtggcggca
     3361 agtgcatagt cggtcgcggg cagtcgagcg ctcgcttcct gcccgggctt actccagcgc
     3421 cacgggtcgt catgcacaag gttcaattga atggtctgcc ggtcacgacc gccaccggca
     3481 acgagcccat cctcgctgcc ctgaagcgca gcctcgtgga gcggacctcc ctgctcatga
     3541 ccttcgtcaa cccgcatgcc tggcggatcg aggaggagac cggcgagcac gcccggctgc
     3601 tgcgccagtt cgacatggtg ctgccggacg ggatcggcgt ggtgcacctg ctgcgccggc
     3661 tgcacgacag ctcggccggc cggttcagct tcgacgccac ctcgctctat ccgccggtgc
     3721 tcgccaccct gcaggaggcc ggcgcctcgc tcctcgtcgt gggcgcgaag ccgggtgtcg
     3781 ccgagcgggc cgtggcgcgg atgcgggcgg ccttcccggg cgtcgatttc ctcgccgccc
     3841 gcgacggcta ccgggattgg gccgagctcg agcgcgagat cctcgaccgg cggccggccg
     3901 tgctgctctg cggcatgggt gctccgcacc aggagcggtt catgctgcgg ctgaaggcgg
     3961 ccggctacgc gggcgtgatg atcagctgcg gcgggttcct cgaccagctc gccgaggccg
     4021 agcgctacta ccccgcctgg atcgaccggc acgacctgcg ctggctctac cgcgtctaca
     4081 aggagccgcg gcggctctgg cggcgctatg ccgtcgatta cctgccgttc ctgcgcttcg
     4141 ccttcgccgc cctcgcccgc cagcgcctgc tcggccccgg cctgcccttg ccgatcgagc
     4201 cgtccgacca gcggaattga accggcaagg cggtgcgtgg ggcatggcgc cggaccatgg
     4261 cattggcccc atgaggtccc tttcacccgc tgtccatagg cactagcttc cgacgcgggg
     4321 ctgccggcct gctggcgccc tgccgccccc atcgagaaaa gcggccgcaa cgacagggag
     4381 cagcaatgac caaccagggc aagctcggga tcggcctcgt cggtgccggc gccatcatgc
     4441 ggctcagcca tgcgccgacc atccaggcca gcgagcgggc cgagctggtc gccgccttcg
     4501 atctcgacct cggccgggcc gaggcgctga cagcggcgtt cggtggccgc gccttcggcg
     4561 atctggaggc gatgctggcg ctgcccggcc tcgatgccgt cgtcgtcgcc acgcccaacc
     4621 gccaccacga ggcggccgtt gtggccgccg ccggggcctg caagcatgtc ttctgcgaga
     4681 agcccttgtc cctggacgtg gccggcagcc ggcgcatgat cgaggcctgc gaccgggcgg
     4741 gggtccggct ccaggtcggg ttcaaccagc gcttctgggg ccaggtggag atcgccaagg
     4801 ccctgatcga cgccgggttc atcggcaggg tccaccagat gcgctcgatc tatgccgaga
     4861 aggccagcgc ctatcccgcc gccaccggct atcgctacga tctcgcccag tcgggcggtg
     4921 cgaccaccat cgacctcacc atccaccgca tcgacctcgc ccggcacctg ctcggggatt
     4981 tcagcggggt gatggccgag ctcgtccaca gcgcgctgcc gcagcaggtc gacgacaacg
     5041 tcctcatgct gacccggttc gcgagcgggg cgcgcggctg cctcgccggc aaccgctact
     5101 cccccaatat cggcgacggc accgacctct acggcagcga gggcacgatc catctggcca
     5161 gcgagacgac gggccccttc acctcggcgc cgctcgccgt ctacacggag aagcccgccg
     5221 ccgagctgcc ggacgtgctg cgcgaggcgc actatcccga agcgtggtgg gagagcttca
     5281 agggtggctg gatcaccgtc cggccgccac gccgcagccc ctacggcaag cagctcgcgg
     5341 ccttctgcgc cagcgtcctc gacggggcac ccgtcagggt gacgggcatg gacggcctgc
     5401 gcgcgcagga attcgtgcag gccgcctacc agtcgatgaa cgagggccgc tgggtcgacc
     5461 tgccgctcgc cgacgacacg ccgttcgtca tccccgccta ttcgtgaaaa ctgccatgcg
     5521 gacctgcttc cacagcgtcg gcctgccgaa ccgccccttg ctcgaggtga tcgggctcgc
     5581 cgccgatgcc ggctacgagg ccatcgagat caattgcgag accctgccct gggcgccgcc
     5641 gcatatcaca cccgcggccg atgccggtgc ccgccgcgcc gtggttgccg ccgccgcgcg
     5701 cggcctgccg atcgcggccc tgggtgccca tgtcgggatg gtgagcgccg atccggcgcg
     5761 acgggacgcg gcccgggcct tcgtcgaagg ctgcatcgac ctcgccgccg atctcggggc
     5821 acccatcgtc cacatcctct ccggcccgat cgacccgggc caggaccgcg agacggcctg
     5881 ggcgtggttc cgggacgcgg tgcgggacag caccgcccac gcagcgaagc ggggcatcgg
     5941 gctcggcgtc gaggccatcg ccggacacct cttccacgag gtcgacgact atcaccggct
     6001 ctacgcggag ctgcccgacg tgcccttcaa ggtgaacttc gacccgagcc atctcgtggt
     6061 gcaggacgag gacccgatgc gcgtcgtcac cgagctcggc gccagggtcg accacgtcca
     6121 cctgaaggac ggcaagggca ccttccccga cttctccttc ccgcctctgg gcgagggtgc
     6181 gatcgacttc cccgctctcg cagcggggct gcggcgggcc ggctatgacg gtgctctctc
     6241 ggtcgagtac gaggcccagg tctacggcta ccggctgacc gaggcggaga tcctgaagca
     6301 gggccgggcg ttcgcggccg cgctgaccgc ctgagcgcgt cgcggcgcca gcgtcggccg
     6361 cgcggggcgg cccgtccccg caacgccggc tcgttcaatg ccggctcgtt caatggaagg
     6421 cggcggtgaa ggtcgtggcg gtgagtgtga cgtgagcgtc gagcggcgga tgcagctcga
     6481 aggctcgcgg ctcgcgcgag aagttccaga gccggagcag gcaccatgcc gagcggcgct
     6541 cgtcggccac ggccagctcg ttgcgggtga tgtggaaagg cgtccgctcc cacccgttcg
     6601 tcgtcttcac ctcgatcagg cgcaaccggc cgtcgggcgc atagctcatg atatcgtagc
     6661 cggcgccatc gccatcctcc tcggacaccc agcgcacctc gcgcaccagg tcgtcacgtc
     6721 ccgccgagcg cagcgaagcc cgctcatgcg ccaagactcg ctcctcgccg gcacggccga
     6781 gcgcacgatt ccgctcgtcc cggccggcaa cgtcgaactt gcgggcgata tgcagcatct
     6841 gctccagctc ctgcggcggc ggctggttcg acaaggtggg cggcgggccg acccacatct
     6901 gcgcggcttc ctcaagaccc agggcagcgc ccgggtccgg caagcaatcg cgccagtcgg
     6961 cgttgcgggc cagccaccgc gccaccgcgt cgaccagcga cgcctggaaa ttgaacgccg
     7021 gcttgtagcc aaggatccag tcctcgccga accccttcag gatcccgctg atgttgcggt
     7081 gcttgaactc gaccgccccc gccgaacggc cggtcaagag cgggagaagc gcccgtcgat
     7141 gctccgtctt gctgtagggg cgcccggcaa tgtcgtcggc cagcatcgcg aaataatccg
     7201 cgacggtccg atcgttctcc tcgtctgccc aatctccgcc cgacattgcg ccaggctact
     7261 gacatcacag ccctttgtca tcgcgcccat gggccggacg ggacccggaa aaacatggtg
     7321 gcccagatca gctcgagggt ggctgctacc ccaggctctt cgtcaccacc tcgtaggcat
     7381 ccttgctcag ccccggggtg gcggcgatgc gttcgagctc ggcgcgcatc agggtctggc
     7441 gggcggggtc gaagcggcgc cagcggccga gggtcaggag gaggcgggcg gcggtctgcg
     7501 ggttcttccg gtcgagctcg agggcggtct cggtgagcag gcggtagccg gcgccgtccg
     7561 ggcgatggaa gccggtgagg ttgccggtcg cgaacatgcc gacgagcgag cgcacgcggt
     7621 tggggttggc gagggtgaag gccgggtggt gcaggaggcg gcggacccgc tcgacgctcc
     7681 gggcgtcctc gatggtcgcc tggagcgcga accacttgtt gatgaccaga ggtgaccgct
     7741 cgaagcgcgc gtggaagtcg gtgagcagcg cctcgtaggg ctcggcctgc acgtcgcaca
     7801 gggccttcag cgccgccagg cggtcggtca tgttgcgcgc ctcgtggtac tgggcggcag
     7861 ccaagggggg ggcgctgtcg aggccagacc agacgagata ggcgagcgcc gtgttcttca
     7921 gggcgcgccg gcccatggcc tcggtggtgg gcgcgacctc ggccggcgcc tcgcggtggc
     7981 tctcgccggc cgcctccagc accctgaccc aggcggcggc gaggccctgg ccgatcgcgc
     8041 ggcgcagata ggtggcgacc gcgtccacac cctcgacgtc gatcaccggc agcgactggg
     8101 cgaggacatt gcggctcggc agcgccaggg cacgggcagc gaaggccggg tcgccggccg
     8161 catcgtcgag caccgcctgc agcgccggca ggatcacggg atcgagcgcc agctccttgc
     8221 cggcgcggaa gtcaccgagc aggccctgca tctggtcgag cagcaggcgc tggccggcct
     8281 cccagcggtt gaaggtgtcg gtgtcgcggg cgagcagggt ggcgagctcg gcgcggccga
     8341 gatccagcgt gagctcgacc ggggcggaga agccgcgcag cagcgacggc aagggccggc
     8401 tcgtcacgtt ctcgaaggtg aaggtctcct tcgcctggcg cagctcgagc acgcgctcgt
     8461 cgccctcggg ccggttctca ccgctgagct gcagcggcat gggatcgccg gtccgccggt
     8521 cgatcaggcc catgcggatc gggatctcga gcggcagctt cctcgcctgc cccggcgtcg
     8581 gcggggtcga ctgctcgagg gtcaggctgt agcggccgct ggccgcgtcc caggcatcgg
     8641 tggcgacgac cctgggcgtg ccggcctggt cgtaccagcg catgaagcga ccgaagtcgc
     8701 ggcccgacgc ctcggccatg caggcgacga agtcctcgca ggtgaccgcc tcgccgtcgt
     8761 gccgcgcgaa gtagagatcc atgccccgac gaaaggcctc ctcgccgatc agggtgtgga
     8821 tcatgcggat gacctcggcc cccttctcgt agaccgtgcg ggtgtagaag ttgttgatct
     8881 ccatgtactc ggcggggcgg atcgggtggg cgagcggccc ggcatcctcg gggaactgcc
     8941 cttcgcgcag catggtgacg tcaccgatgc gcttgaccgc ggcggagtgc atgtcgctgg
     9001 tgaactgctg gtcgcggaag accgtgaggc cctccttgag ggtgagctgg aaccagtcgc
     9061 ggcaggtgac ccggttgccc gtccagttgt ggaagtactc gtgggcgatg atccgctcga
     9121 cctggacgaa atcggcgtcg gtggcggtgg tcgggtgcgc cagggtcgcc gaggtgttga
     9181 agatgttgag gcccttgttc tccatggccc ccatgttgaa gtcgctgacg gccacgatca
     9241 tgaagaggtc gaggtcgtat tcgagcccgt agcgctcctc gtcccaggcc atggatttct
     9301 tcagggacgc catggcgtgg tggcactggt cgatgagctc gtgctcggaa tagatccgca
     9361 gctcgacggt gcggccggag cgggtggtga agtggtcccg gagcagggcc aggtcgccgg
     9421 ccaccagggc gaagagatag gcgggcttgg ggaacgggtc gtcccagagc gcccagtgcc
     9481 ggcccttcgg caggtcgccc tggccgatcg ggttgccgtt ggagaggagg tgcgggtagc
     9541 gggccttctc ggcctcgatg cggacgcgaa agcgcgtcat gacgtcgggc cggtcctgga
     9601 accaggtgat gcgccggaac ccctcggcct cgcactgggt gcagaagatg ccggacgaga
     9661 tgtagaggcc ctcgagggcg gtgttggcgg cgggcgcgat gcggttcgtg atctccagcg
     9721 tgaagcgctc gggcgggtcc ggcagggtca ggcaggtggc gtcgagccgg taggcctctg
     9781 gtgccagcgc cgcaccgtcc atcgcgatcc gcacgagctc gaggccctcg ccgtcgagga
     9841 ccagaggcgc ggatgcgccg gcacggcggg cgaagctctg gcgggcggtg acggtggtgc
     9901 ggtcgggctc gaggtcgaaa tccagctcga cccgcacggt cgagaaggcc ggcggggcat
     9961 agtcggcaag gcgaacggtg cggcgctcgg gcgctcggct catcatcgca ttctcgcgcg
    10021 tcgggtctcg cgggtcggct tgtcggaccc ttggtgtgcg attagcttcg cccgcgcaag
    10081 gccggaaacc ggcccacgcg tttcaatctg cacgaatgga aggaagcgag aatggccaag
    10141 accgccttta tcgggctcgg ggtgatgggc tttccgatgg ccgggcacct ggccgccaag
    10201 gggcacgagg tcgccgtcta caaccggacc ggcgagaagg ccagggcctg ggtggccaag
    10261 catggcgggc gcgcggccgc cacgccgcgg gccgccgccg agggtgccgc ggtggtgatg
    10321 atgtgcgtcg gcaacgacga cgacgtccgc tcggtagcgc tcggtgacaa cggcgtgctc
    10381 gccggcctcg acgacggtgc ggtgctggtc gaccacacca cggcctcggc cgagctcgcc
    10441 cgcgagctgg acgcggcggc cggcgagcgc ggctgcggct tcgtcgacgc gccggtctcg
    10501 ggcggccagg ccggtgccga gaacggcatc ctctcgatca tgtgcggcgg caagccggcc
    10561 gatttcagcg tcgccgaagg cgtgatgcag gcctatggcc ggaccatccg gctgatcggc
    10621 ccggcgggct cgggccagct caccaagatg gtcaaccaga tctgcatcgc cgggctggtc
    10681 caggccctct ccgaagggct gaacttcgcc caggcggcgg gcctcgacac ggacaaggtg
    10741 ctcgaggtgg tctccggcgg ggcggccggc tcctggcaga tgaacaatcg ctggaagacc
    10801 atggccgagg gcaagttcga cttcggcttc gccgtcgact ggatgcgcaa ggacctcgcc
    10861 atctgcctcg gcgaggcgcg ccgcaacggg gcggccctgc cggtgacggc tgccgtcgac
    10921 cagctctacc ggctggtgca gcagcagggc ggcaagcgct tcgacacgtc ctcgctgatc
    10981 ttaaacctgc gcgccggcaa gtaggggccg ggacgggccc gcacttggcg gtcttgcatc
    11041 gcacaccata tttccagtgc cggggtcgga cggcacgcac cgcccgacac ccgaccggca
    11101 agagctgctg gagtgatgga gaatgcaagc gagagccgcc aagttcggca tcggccagat
    11161 cgtcaagcac cgcatgttcc cgtttcgcgg cgtgatcttc gacgtcgacc ccgagttcgc
    11221 caacaccgag gaatggtaca acgcgatccc ggaggaaatc cgtccggcgc gggaccaacc
    11281 cttctaccac ctgctcgccg agaacgccga ggccttctac gtggcctatg tgtcggagca
    11341 gaacctgctc gtggacgcga ccggcgagcc ggtcaaccat ccgcagatcg gcgagctgtt
    11401 cgacaatctg gacgcgggcc agtacacccc caagcgtgtc tacgcgaatt gagcaggagg
    11461 gtcggggccc ggccctggag atgacatgaa gttcttcatc gatacggccg acgtggccga
    11521 gatcagggat ctggcagcca ccggcctggt cgacggggtg accaccaacc cctcgctgat
    11581 ccgcaagtcc gggcgggact tcatcgaggt gatcgccgag atctgcgagg cggtgccggg
    11641 gccggtcagc gccgaggtgg cggcgaccac ggtggacggc atgctcgccg aggcggcgcg
    11701 gctcgtgccg atcgccccca acgtcacgat caaggtgccg ctgacctggg acgggctgaa
    11761 ggcgacgaag cgcctctccc aggacggcgt gaaggtcaat gtcacgctct gcttcagcgc
    11821 cgcccaggcc ctgctcgccg ccaaggcggg agccgccttc atctcgccct tcgtcggccg
    11881 gctcgacgat atcggccagg acgggctcgc cctgatcgcc gacatctgcc agatctacag
    11941 ccagtacgag gcgatcacga ccgaggtcct ggtcgcctcc gtgcgcaccc ccatccatgt
    12001 gatcgaggcg gccaagatgg gcgccgacgt ggcgaccctg ccgccggccg tgctccgcca
    12061 gctcgtccag cacccgctca ccgacaaggg tctcgccgcc ttcctcgccg actgggaagc
    12121 gaccggccag cgcatcgttt gagaggctca tctttcagtt gatcatctgt ggttttttga
    12181 tcaactttta gtagaaaacg tgctagagcg ttcggctcgg agcgaatccg accgggcgga
    12241 cgacccggcg gcagagcagc gggggctagc atgagcgagc ggacggcgtg ccgcgtccag
    12301 tgggtggcgc cgagacacga tctcggcagc attctggaag caatggtgag cgacggcggg
    12361 cggctggtgg cacgcagcgc cttcgagccg gagacggccg agctcgacga gctgggcgac
    12421 ggcgagctcg gcgatccggc gatcggcctc ggcatcatgg aggtcagcgt cgacccgacc
    12481 agcctcgcgc ggcggatgtc cgccgcggtg gcggcgcagg ccgaggccga gaccctggtc
    12541 gtcgacttca ccggccggcg catccgcttc cagatcctgc cggtcggcgg cgaccacgcc
    12601 gtgatcgtgc gcgccccgga aggcgtgcag cgcttccggc ggcgctcggc cggccgggcg
    12661 atgggccata tccggcggcg cctggtcgat cgcctggccc gccccggagg cccatgatga
    12721 cgaaggaaca gggcacgccg accgtcgaat ggaacgagac ggcggccatg gcgatcggcc
    12781 ggatgcgcgc cgccgacgtc gcctcgctcg tggtgatggg cgggcgcggc cggctggggc
    12841 ggatcacgct ggccgagctc gagcgctgcc ggagccaggg caactggctg gacgccgtgc
    12901 tggtgcgcga cctcgttcgc ctgccggcga ccccggtccc ggtggagcag cgcgcctagc
    12961 gacctggcgg ggcgccgggc ggcggctcag ccgatggcgc cgatatggtg cagcacgccg
    13021 ctgccgatgc ggcggatgac atcgtaatag gccgcgatcg ggcggaagat cagctcgtgc
    13081 tcctgggcat aggagtggct ctccgtgtcc cgataggagg cgggctcgcc gaagtcgagg
    13141 cggcgcggct cgttggtcag cgggaagacc tcgctcagga gctccagcgc cggctcgacc
    13201 tcgagccgca tcaggctgac gatcacctcg tcatgggtgg tgttgtggcg gtcgctgaaa
    13261 tcggggacgt gaacggcggc ctgcatgagc gcctggatca cggcgatcct gagcgcgtgc
    13321 atcgagttca ggttgttgcg ggtctcgagg tcgacgacga tcggctccgc cccggcgtcg
    13381 cgggtcagcc gccggtgctc gcgcagcgcc cgggcgaggt cgatatagtc gcggatcagc
    13441 gtccggctga cccggccgag ccgatcgaag agcgccatgc gctcgaccac gcccgagacc
    13501 tcgcggagcg tttcctcgcg caccgggtcg ttctcgagct gggcgaggcg cagccagtcg
    13561 cccgggctga agaggccgag ataggccacg agcacgtcgg ggtcggtgaa cttgaaggcg
    13621 tgctcgacca tcgtcatcag ccggcggaac cgggcgctct cgcggtacag ccgctggaag
    13681 atctgcgggt ccttgtcgac cgcctggccg accccgccga tggtgttggc gaggatgccg
    13741 agctgctgca ggatcgcgtt gtgcgggatg gcgcggagct gcgaggggtg ctcgagcacc
    13801 gccgcgacgc tgccgcggtc gtgctggcgc ttcagcgcgc gcgagcccgt ggggtagagc
    13861 atgttggtgc cgaaggcgcc gagcagcgtc gcgtagcagg gatcgtcgat gatccgggcg
    13921 ttgaactggc ggatcgccgc gaagaactcg tcggtgtagg cggcctcggc gtagaacggg
    13981 tccgctgccg cgtcggcggg ctcgaggcag tgctccagca tgcgggtcag caccgcgagc
    14041 gcgccgtgct cgtgcaggaa gaagaggtag ccgtcgccac cctggaagct gctctcctcg
    14101 gtcagcctga cgcccgcggc ctcgagcagg cggcggcatt cgggggtgtc gtagtagagg
    14161 aagcggtcgc gcaggctgtc gggatgggcg ccgcgcccga tgctctcgcc atgcgtgtcg
    14221 aagaccacga gctcgatatc ggcgaggccg tgccgggtga gcaggtcggc gaggccgagg
    14281 cgcagccgct cgatggcgaa ggacgcggcg atctgcccga gatagcggcc ggcatcggag
    14341 aacccggtct ggatgcacat ccgcccgcgg ccgcggagat actcccgata ggccggcacc
    14401 tcgagcgccc cctcgagcac ctcgatgccg cggtccaggg ccttgatggt ctcgaacagc
    14461 ggcgagatgt cgatgtgccg ctcgaccccg aaccgcttgg cgaagtagag cgcggtgagc
    14521 agcgtgaacg gggtctcgca ctcggcgatc aggaagcgga tcggctcgct cgaatcgaga
    14581 tgcttcagca tctgcgcgac cagcatgagg acccggcgcg ccgttgtgtt ctcggcggcg
    14641 accgtgccga agttcaccgt ctccggcacg gccgcgtcga tcatctcgac caccgcgttc
    14701 cggtagctca gccggaagac cggatcgtcc gccgcgtgat ccatgtcgat cgacttgcgg
    14761 atggcgttgt gcagctggac cgcgttgatg cggacatggg tccgcgccgc gctcaggccc
    14821 tgggtggcga tctcggcgcg cagcacccag agctcgcggc gggtcgccgg atcgtcggct
    14881 gcgcgaaggg cccgctcgag cagcgcctcg agctggcggg catcggtcag ccggctcggg
    14941 cccatgcggt gcatctcgcg cgacatcgcg gcgagccgct cgagcgccgc cgggtccctc
    15001 gggtcgaacc cctcgaagac ccgcagctcg tcgcgcagct ccttgtcggc gagggccagg
    15061 cgggcggaga tcagctcgag cacgggtgcc gcggcgccgg cgcccgcgcc taactggtgc
    15121 agggcgccga tccgcctgac gacgtgctcg aactggtcga tctgcaggcg cacgcgcttg
    15181 gcgtaggtgg tggcccagga gatgtccgaa cggccgtcgg tgtcgtagcc gacccagctc
    15241 gcgaggctga cgaggcaggg ggtcacctcc tgccagctct cgggatagtg cgcgtggacg
    15301 acgtcgaaga cgacgccgta gacggtgcgc aacgcgttgc gcaggttgtg gatcacctcg
    15361 accgactggg cgtgctcggc gtcgaggtcg agccccggct cggggcgatg gttggcgcgc
    15421 tcgacgcgct cgagcagctc ggcccgcgcc gcctcgtcga gcgcctcgcc atcatccgcc
    15481 cggccgatgg cgaggcggat gaggtcacgc tgcagcggca gggccaggct gaacgtgggg
    15541 tgcgccgtca gcaccagccc gtagagccgg cggccgagtc gcgcctggag ctgggcgacc
    15601 ggcaggagct cgccggccgg gtcgcgcacc agccgctcga tcatctccac gaggcaggcg
    15661 cggttcttcg ccgggtcggt ctcgccgaga taggcgtcga tccgtcgggc gcgggcggcg
    15721 aaggcccggg cgatcagccg ctgcacgagc tggcccagtg tctggcggtc gatggcgccc
    15781 gattgcagct cggcgcgcag ctcgccggcg agcagctcga tcgggttggc ggccgggtcg
    15841 acctcgaccc gctgccggtg cgtctcgaga cgctcggccc actgggcgat ccgcgcgtcg
    15901 atgccgcccg ccttcggccc cgccatgggc atgatccgcg ccgtctcgct catcctgttc
    15961 cccgtttccc gccgccctgc caagaagcaa gaaccctgcc atgggtcggc cgccccgcaa
    16021 agggccgtgc tcgacattgc tgcaatgcaa cagaccagcg caacagaaat gtcgatgccg
    16081 ggcaccctgc ccgggctctg atcatggccc gccgccgggg cgggcacggc acgaccatca
    16141 cgggtccggg caattcctct cgttgtcccc cggtcacagt cgtcgggcag acgacagcct
    16201 ccggacgggc ggggcttggg ggtgccgccc gatccgcgat agattgccgg ccgcgcaatg
    16261 gagacgcgcc ccgagctggc ggtgcgtccc ggatacccat ggtttttgcg acgagttgga
    16321 ttgcagtggc caagtacagt cgacgggcgg cgatcgtcgg ggcggcagcg ggcatcgccg
    16381 gcgccctggc gccccggtcg ggcttcaccc aggagagcga ccagcagatc ctgatcgacc
    16441 gggcgcggct cgtcgtcgag gaattccagt ccgaccccaa cttcgccaat gtcccgatct
    16501 acgtgcagaa cgcctatgcc gtgatgatcg tgccggacat gctgcaagcc ggcctgatca
    16561 tcgggctcga gcacggcgtc ggcgtgctgc tcgcccgcaa ccgcacgacc ggtgcctgga
    16621 gcgatcccgc cttctacgag ctctacggcg gcagcctcgg cctgcagctc ggcggcaagt
    16681 cgtcggacgt gatcttcacc ctgatgaacg ccaccgccgt cgacaagctc ctgacctcgc
    16741 agttcaagct cggcgtcgat gccagcatgg cggtcggccg gatcggcgcc ggcgtgggtg
    16801 ccgcgaccac cgtccgcttc ggcgaggaca tctacgtctt ctcgcgcggc cgcggcctct
    16861 atggcggcct cgccgccgac ggctccgccg tgctcgagct caccgagtgg aacgaggcct
    16921 attatggcgc cggcgtctcg gccgggcaga tcctccgggg cgagctggcc cagcgcccgg
    16981 gtgcgcaggg cctgcgcgag gcgctggccc ggttctagtg gctgatgcct atggctcgaa
    17041 gcgccgggcg ccctgcgagc tgtagggcct gagccctttc gccccgtatt tgagcagcca
    17101 gggcttccga cccttgcgca ccagcagccc ggccgcatcg agctcgcccg ggatcaggtt
    17161 cggcaggacc ctcgggttcc gcgcgatgta gcggagcgcg ctgggcgcgc gcatgaagag
    17221 gccgcgcaga tcggccttgg gaatcgcctg catccacggc tcggtgacgc cgagcgccgg
    17281 ctccggcagc ttcgccgggt cgacaccgtc cgcgagaccc cggtcgacca gccgccgcgc
    17341 cgtcaggagg accgtgtagc gcgccatggc ggcacacgag cggaccagcg tctggtaggt
    17401 cagctcctcc ggcgccagct ccgggccgtg gaaggccagc gggcccggcg tctcgatccg
    17461 ccggcgcagc gcctcccagt cgtcgcccgg cccgccatcc gcttccaccc ccgcccctgc
    17521 cggcgccgcg ccgagccagt ccgcgggaac ctggtcgagg aggcgggcca gcgtgtgccg
    17581 ggcggctccg tcgcccatgg ccagcgccac ccggagcagc tccagggcac cttcggcgcc
    17641 acccggcaga cggagccggt agctccacca ttcgtggccg tccaggacat cctcgagata
    17701 gcgctgggcc ccggcatagg ctctgaggca gaggtcgaaa tcgtcgccga gcgccttgac
    17761 gaagctgaac cggtcgtcgc ggaagcgcca gggctcccag ctcagcacca gatcgtcgag
    17821 ccgggcgtcg gcgccgaccg gcgagccgtc gaggtccagc atcaccggcc ggcgggccgc
    17881 ctcgaagacg aagcgcagat ggccgtgctg caccggcccc cggccgccgc ccgggccgag
    17941 cggcaccatc gtcagctcga catgggccag accgtcgagc ggcatccgca cgctgtgcag
    18001 caccgtcgtc cgctcgagcg ggctgccgcc gtaattgacg ttgcgcagga ggagaaaggg
    18061 cgcctcgccg tcgagccggc tcgatcgcca ctcgaccagc accggcaccc gcgccgccag
    18121 atggcccgag ggcaggaggt cgcggttggt ccgcagccgc atgacccggc tctcgtcctc
    18181 ggtccaggcg cgaagctgcc gggcggtgag ggtgcggtag acgtcgtcgg agctcatgac
    18241 ggcacggggc ccggcgcggt ggatcggccc gcgaacctag caggtgatgc ccccctcggg
    18301 cgagatgcgc ctggccggtc ccgcccgggg cctcctactc ctcggcgagc acccggccgg
    18361 tcgcctgcac ggtggtcgcc tgccagcctt cggggctctc gttctcggcc acctcgagcc
    18421 gcacctcggc gccaacggcg agggcgtcgt agccgccatc ggcgacgctg ttgcggtgga
    18481 aatagacgtc gcccgccggg gtctcgacga agccgtagcc ctcgtcgggg aagaggcgga
    18541 cgacgcggcc ggtgagtggc gcctcgtgct gcttgacctt gcccgccacc gaccggctgc
    18601 gctcccgaag ctggcgctgg gccgccgcga aggcatcgcg gaccgccaca tacacgtcct
    18661 cgtgcgcgtg gtccttcggc cccgcccggc tcaccgcgat ctccttgccc ggcatgccga
    18721 tatcgagccg cacccggtag agattgccct tggtctgatg acgatcctcc ttctcgacca
    18781 ccacccggca gctcgtcatc cgcggggcga cgcgctcgag cgcctcgacc ttctcgcgga
    18841 tccgcgcctc gaccgccggc gagggatcca tctgctggaa gcggatctcc aagggcttct
    18901 gcatggtgtc tcttcctcct cgttcgtcgc tgaccgaagc tgccccgatc cgcgagacca
    18961 ccgcatcgat cccgcgcggg aaagcggccc gcaacaagag acgttgcgcc gatcgacgct
    19021 tctggcaagg ccattcggca cgccgccgcg ggcggcggtc ccgaagcagc gaaaaccccg
    19081 ccggcactgg gctctggcgg ggttttccgg cgctatggcc attgccgacc gtcgtcggcg
    19141 ctgtcgcgaa gcgcgcatcc agcgacgctt cggcttgtcg gctcgaattg tctggcggcc
    19201 cggatcatct ccggaatctg cacttccggt cgttcagccg gaccgtgatc ccgcggctcg
    19261 cggcggcggg atcctcacga actcccgggt ggatcttcca tgcgcattcg caccctcccg
    19321 ttcgctggtt gccactcgct ggcggcgtct tcgaccctgc gcgccgaccc ttcggctggc
    19381 ccgcttcatc tgccggcggg gacggcattt ccgacggccc tggcgcggca tgggaacgac
    19441 accaacaaat ttatactagc aaactcgcgg ccccggcaca aggcggcatc gccttgaagc
    19501 agccggtggc gccgactacc ctgccctctg caacgatcgg cggagaccac catggcacat
    19561 gcatgcccga cctcgttcgg ccttctcggc ctcgctctgg caacggttgc ggcggcccag
    19621 gtcatggccc aggaaagcgc cgtcgacgtc gtcgccgtgg cggtgcgcga cgccggccat
    19681 gcctgcaccg atcccaggga cgccgtccgc gacgcggccc gctcggcacc gaacgaggcg
    19741 gcctggacga tccgctgcga cgagggcgcc tacagcgtca ggttcatggg cgacactggc
    19801 gcgaaggtcg agcctattcc ctgaatcgcc gctgcccggg ccgcaatcgc cccgctcaag
    19861 acccccgaac gcgtcgtccc agcttgtgga tccagcgctc ggccagattc tcgcccgtgg
    19921 tgaagacgct gcccaggcgc gggaagcggg ccttgaaccg ggccagcagc cgttcggccc
    19981 agggcgcatg gcgcgacagg atgacgaggc cgacgacgag gaagagcacg ccctgcagca
    20041 ccggcaggaa gagcccgagc acgcctagga cgagaaagcc gtagcccagg cccagcacca
    20101 ggagccggct cacgtcacgc ccgccatggc gagccaggcc gcctcgtcga gggtctcgac
    20161 accgagctcg accgccttcc tcgccttgct gccggcatcc gcccccagca ccacgtaatc
    20221 cgtcttcttc gacaccgacc ccgccacctt cgcccccagc tgctcggcca gcgccttggc
    20281 ctcggcacgg gtcatctgct cgaggctgcc ggtgaagaca agggtcttgc ccgaaaaagg
    20341 cgagccgccg ccggccggcg ccgccacggg ctcgaccatg acctcgcccg cgaggtcgtc
    20401 gagcgcggcg atattgtgcg gctcctggaa gaactcggcg atggcctcga ccagcctggg
    20461 cccgatgccg tcgatatcgg cgagctcggc ccgggcatcc tcgtcgcccc cggcaacatc
    20521 cagcatggcc tgccgccacg cctccagcgt gccgtagtgc cgggcgagca gcctggcgtt
    20581 cgcctccccg atgaagcgga tgccgagcgc caggatgaac cggtcgagcg ggatcgcccg
    20641 ccgcgcctcg atcgccgcca ccaggttctc gatcttcttc ttgccccagc cctcgagctc
    20701 ggccagcgcc tcctgcttca ccgggtcgcc ggcgaggcgg aagatgtcgg ccggcgacct
    20761 cagcagcccg gcctcgatga gctgcggcac ctgcctcctc cccaggccct cgatgtcgaa
    20821 cgcgtcccgc ccgaccgcat gctccagccg ggcctcgacc tgcgccgggc agatcagccc
    20881 gccggtgcag cgcctgaccg cctcgcccgg cggccgcacc gcgtggctgc cgcactcggg
    20941 gcaggtctcc ggaaagacga agggcgcagc ccccttcggc cgccggtcgg tcaccacctc
    21001 gaccacctgc gggatgacgt cgccggcgcg ctggatgacc accgtgtcgc cgacgcgaat
    21061 gtccttctcc tcgatatagt cctggttgtg cagcgtggcg cgcccgacca cgaccccgcc
    21121 cacggtgatc ggcgcgagcc gggccaccgg cgtcagcgcc ccggtgcggc cgacctggat
    21181 gacgatctcg tcgaccaccg tcaccgcctg ctgggccggg aacttgtggg cgatcgccca
    21241 gcgcggcgcg cggcccacga agcccagccg ctgctgcagg cggagatcgt ccaccttgta
    21301 gaccacgccg tcgatgtcgt agccgagccc ggcccgccgc tcgccgatct cggcgtggta
    21361 ggccagcagc tccgccgcgc tccggcagat gcggttcaaa ggattgaccc tgagccccat
    21421 gccggcgagc cgttcgagga agccgtgata gctgccggtg accggcggct cggcggcacc
    21481 ccagccatag gcgaagaagc gcagccggcg cgtcgcggtg atggcgctgt cgagctggcg
    21541 aaccgagccg gccgcggcat tccgcgggtt ggcgaagacc ggcagctcct ccttcgcccg
    21601 ccgctcgttg agctttcgga actcctcgag ctccatgaag atctcgcccc gcacctcgag
    21661 cacgggcggc gggtccccgg tgtcgaggcg ctgcgggata tcgcggatcg tccgcacatt
    21721 ggcggtgatg tcctcgccgg tcgtgccgtc gccccgcgtg gcgccggtga cgagaagacc
    21781 ggcctcgtag cggagcgcgc aggagagccc gtcgatcttg ggctcggcca gaagctcgac
    21841 cggggcatcg gcggcgaggt tgaggaagcg gcggatgcgc gccaggaact cgaccacggc
    21901 ggcatcttcc atggcgttgt cgagcgacag catcggcacc gggtggacca ccttgccgaa
    21961 cgcctcggcc ggcgccgcgc cgaccctgag gctcgggctg tccggccgca ctagcgccgg
    22021 gaaccgcgcc tcgatcgccc ggttgcggcg gaccagcgcg tcgtaatcgg cgtcgctgat
    22081 cgccggcgcg tcctcctggt gatagagccg atcgtgcctg gcgatcaccg tcgcgagacg
    22141 ggcgagctcg gccgcggcgg ccttctcgtc gagcgcttcc accgcgacct cctccggccg
    22201 gctcaccgca cgccccccgc atccaaaagg ctcgtcgccg cggcgcgcgc ggcggcggtg
    22261 atctcggcac cggccagcat ccgggcgatc tcgtcgcgcc gctcggcggc atccagcggc
    22321 cgcacctcca ccctggcgcc ctccatgtcc gtcagcttct cgaccttcag atgatggtcg
    22381 gcacgggccg ccacctgggg cgcgtgggtc accaccagca cctggcgttc ccgcgccagc
    22441 cgggccagcc gctcgccaac cgcgtcggcc gtcgccccgc cgatcccggc atcgacctcg
    22501 tcgaagatca gggtgccgct cgcggcgtcc cgggcgagaa ccaccttgag cgccagcatc
    22561 agccgcgaca gctcgccgcc cgaggcgatc ttggcgatcg gcccgagcgg ctggccggga
    22621 ttggtcgcga ccaggaacgc gacccggtcg agcccgtccg gccccgcagc ctcctcgtcc
    22681 agcggctcga gccggatggc gagccgggcc ttgtcgagct tcaggggcgg cagctccgcg
    22741 gccaccagct tctccagcac gcccgccgcc gcctcgcggc tccgacggag cgcccgggcc
    22801 acctcgagat agcgcccgcg cgccgcccgg gccgcccgct ccagcccttc gagcgcttcc
    22861 gccccggcat cgagcgcccc cagctccgcc gccgtccgtt cgagcagggc cggcaggtcg
    22921 tcgaccgccg tccggtgctt cctcgccgcg tcgcgcaacg cgaagagccg ttcctcgacc
    22981 gcctccaggg agcgctcctc ggcggcgacc tcgcgcaacg cctcctcgat ctgggcctcg
    23041 gcctcgccgg cctcgatcag cgcccgcccg agggcggcga gcgccggctc gacgagggac
    23101 gtcgccacct ccggggcgcg ggcgaggcgc cgctcggcgc tccccagcct ggcgagcgcc
    23161 gcctccggcc cggcgacgag cgccagcgcc tcctcgaggg cggtcaccag cttccccttg
    23221 tgcaggaggc ggctgcgctc ctcggcgagg cgctcctcct cgccggcctc gggggcgagg
    23281 gcctcgagct caaggagccg atggcgcaga tagtcctcct cgcgacgggc ctgctcgacc
    23341 cttgcccggc tctccaggat ctccgcctcg aggccgcgcc aggcggcatg cgcggcacgg
    23401 gtcgcggcga gctggtcgcc gtggccggca aaggcatcga gcacggcccg gtggctccgg
    23461 ctgtcgagca ggccgcgctg gtccatctgg ccgtgcacct cgaccagggc atcgccgagc
    23521 tcgcgcagga tggcaccgct caccggcacg tcgttgacga aggcgcggct gcggccgtcg
    23581 gcggcgagcg tgcggcggac caccagctcg cccgcctcgc cggccagctc ctgcccggcg
    23641 agcagggcca ccgcggcatg cccggccggc ggcctgaact cggccacgac cgagccctgc
    23701 gccgcgcccg accgcaggag aacccggtcg gcccgctggc cgagcgccag gcccagcgca
    23761 tcgaggatga tcgacttgcc ggcgccggtt tcgccggtga gcaccgtgag gcccgggccg
    23821 agctcgagat cgagccgctc gatcagcacg atgtcgcgga tggcgaggct cagcagcatg
    23881 gcccgggccg gcgccggccg cgcgcagcgc tgcggggggc gctcagaaga gcccggtcag
    23941 ccacgagccc tcgctcgcgg gggcgccggc ctcctcctgc tgcatcaggg cataggaccg
    24001 ctggtaccat acactgtccg gataattgta gccgagcacc gccgccgcct cctgcgcctc
    24061 ctggaagacg cccagggcga gataggattc ggtcagccga tgcaaggcct cgggcacatg
    24121 cgtggtcgtg tcgtagttct cgaccacggc gcgaaaccgg ttgatcgccg ccacgaactc
    24181 gccgcgcttc tggtagtagc gcccgatctc catctccttg ccggccaggt ggtcgaaggc
    24241 gaggtccatc ttcagctcgg cgtcgcggga atactcggaa tccgggtagc ggcgcaccag
    24301 ctcgccgaac gattccagcg cttcctgggt catctcctgg tcgcgaccga catcggagat
    24361 gcgctcgtag taggacatgc cgatcaggta gtgggcatag ggcacgtccg gattgcccgg
    24421 atgcagctcg atgaaggtgc gcagcgctgc gatcgcctcc tcgtagagct ggccctcgta
    24481 ataggcatag ccggccagca cctggccgcg caccgcgagc tgcgaatagg ggtgctggcg
    24541 ctcgacctcc tcgaaggccc gggccgcgtc ctcgtggttg ccggcggcca tctgctgctc
    24601 accctcgctg aacagctcct cggccgtccg ctcgacatag tcgtccgcgc cgctgttgcc
    24661 gccgcaggca gcgagcagcg gcgccagggc gacgagcagg gccagcacca gccggcgcgc
    24721 atctggcagc cgtcgaccgg cagatgcgtg gccggccgac ttcaatccgg tgatggaagc
    24781 gaacatcgat aaccttccga tagccgccga ccgctagagc ggcaccggcg gaacgtggcg
    24841 agtcggcccg gaggacgggg ggaccccgac tgcgcgtagc gcgcgataag ctaccggttt
    24901 gtcgagccgg acggaagcct gtccgatgct cagcgcgcgg ccgcccgctc gatggcggca
    24961 agcagcggca gccagtcctc gcgcccggcc gccgcccggc gctggtcccg gcgctggtcg
    25021 aggtcgaaga gcccgagccc gcccatcgcc agctcgccat aaagcgtgcg ctcggcgagc
    25081 cgggcggtgg gctcgagccc cgcctcggcg agatgctcga gcagatgctg cgccgctttc
    25141 tggcgcggcc gcaggcgatt ggcgacgacc agcacgcgct tcctgcccct ggcgatcggc
    25201 ttgatgctct cgagcttctc gaggaagcgg ctggtgctgc gctcgtcgaa gaccgagggg
    25261 agcaccggca cgatcaccac gtcggccgac ttcagcaggt cctggacgtg accgagccgg
    25321 agcccggcag gcacgtcgat gaccagccgc tgcgtccccg cgggcttcga gtccgctgcc
    25381 ttgcgccagt cgaggccggt gatgggtgcc gcggtggcgg gccgggtggc gagccagccg
    25441 aggctgctcc tctgccggtc cacatcggcg agcgccgtct tgaacccagc aacggcgaag
    25501 gcggtggcga gcgtcgtcgc gatggtggtc ttgccacacc cgcccttggt gttggtgacc
    25561 agaacggaga ggctcaacgg tcttcatcca gttcttgcca gaagagctcc tgcgcggtga
    25621 agtcgtgcca atcgtcgacc gccagccgac gttcggcgcc gacgcaagcg gcctgccagc
    25681 cgatgaccag gccgatgccc cgctcgagcg ccggccgatc cgtccccggg ccggccgagc
    25741 ctgcgagcgc ctcgagcagg cgccgcgtgt cggcctggtc gttgagccgg ccgagccggt
    25801 cctgcagcct ggccgccgcc ttggcgtagc gtttcgccgg cttctcggga aagagaccgc
    25861 tcaggaactc gacaccgtag cgcagcttct tgagagccag ccgtacctcg tgccgctcct
    25921 cgtcgccgag ccgggcgaaa tgccggccac gcctgcgcac ccgcttgtgc cgccgctcga
    25981 gcagcgcgac cgcgagctcg atcaccggcg tcgactgggc cgcagcacac tcttccgtgg
    26041 tatcgtcgcg ccagcgctcg agcgccgtcc agagcgccag gtcgagcacc agaccggcga
    26101 gctcgcggga ctgcaggtag gtgcgcaacg tcgcgtaggc gcggcgccgc gccgcctcgg
    26161 ctgtcgcggt gagcaccgcc agggccgggt cgtcgggcag gcccgccttg atctccgcca
    26221 gcgtctcggt gaccagcacg tcgagctcgc gcggcgtccc ggtcgccgcg atcaccgcct
    26281 tcagccgctc gtcccaggcc gtgcgctcgt cgacgggcag gagcgaagcg aagagggcca
    26341 ggcccgagcg gatgcggcgg agcccgatcc gcagctggtg cacgccctcc gggtcgcggc
    26401 catcctgcgc cgcggcctgg ttctgcagga gctgctgcag gccgctcgcc agcactgccg
    26461 ccatcgccgt gccggccggt atcgccggat cgagcgccgg cggctcggcc cgctgccacg
    26521 ccggcggccg acccgtggcg agcaggtagc cgcgctgggc tttgggcgtg gtgctgatgc
    26581 gcagcgtcgc ccagtcgctc agggtctcga gcagcagata gagcccctcg ggcgctcccg
    26641 ccttcagctc cagctcgacc tcggcgatct gctcgacccg tgccttcttg ccggccttgc
    26701 ccgccccggg gccagtcgcc agggcatcgg cctcgatcgt gccccggtcg aaggccacct
    26761 cgatgatcgc cttgctgccg tcgcgcagct tctgctcgac cagcatcagc cggcgctcga
    26821 cccgggtcgt gaagaccggg gccagctcct cgggcaggac caggccgagc gcctccagcg
    26881 ccgccggatc gccgatgcga tcgagctccg gcgccatggc ggcgacggtg cattcccatt
    26941 cgccccgcac cgtgtgcgcc gcaccgccga ccgacgcggt cttgatcgtc tggacatgtc
    27001 gctcgccgga ccggcggacc cgcaggctga cccgacgcgc ggcgaggcgc aggtccggcg
    27061 tgtcgaagta gcggctctcg agcggcagca cctcgggcgc ggagaacgcg aaggggctcg
    27121 cccccaggcg cgcggcgagc ctggcgaggt cggccgggtc gaccatgagc ttcagctcga
    27181 tctcatggtc gcaggatggc gcgcgcacgt cagagcctcc gaatcccgtt ggcgcaagtg
    27241 gtcgaagaac ccggcgttca gcgaccctcg tccactcccc gcatattctg ctagacttgg
    27301 gtagcggtgt cgctgaccca gatcaatgcg gcaggatcag cgcccggcta ggaattctgc
    27361 cggttgtccc gatcggggtg gaggagcgca agccatgagt ctcaaggatc tgctggtgca
    27421 tatcgacgac aagccgagca gcgacaagcg cctcgatgcg gcggtggcac tggcgctcgc
    27481 ctgcgacgcc catctcgccg gcctgatgct ggtcgccgag ccctatgtgc cggccgcgat
    27541 cggcgtcagc attccggccg aggtgcttcg cgagcagcgc gaagcggccg tgcagcgttg
    27601 cgacgcggtg ctggccaagg ccaaggcgcg cgccgaccag gccggctgtc ggctggaggt
    27661 gcgccgcgag gtggtcatgg tcgacgattt cggcacggcc ttcgcacgcc aggcacggca
    27721 tgcggatctc agcatcgtcg gccaggcgga tccgaacgag ggcgatgtcg acgccgagct
    27781 cgtcgtcgag gcgagctttc tcgacagtgg ccggccggcg ctcgtcatcc cctatatcgg
    27841 cgcccgccag atcccgccga agcgtgtcct gatcgcctgg gacgggtcgc gcgaggcggc
    27901 ccgggcggtc ggcgacgcca tgccgctcat gcgcaaggcg gagagcgtcc tcgtgctggt
    27961 cgtcgatccg gcgaagctga agggtcgcat cggcgagcag ccgggcgccg atctcggcga
    28021 gcatctggcc cggcatggcc tgaaggtcga ggtgcggacc gcggcgagcg gcggccaggc
    28081 ggtcggcgac gtcatcatcg gccaggccag cgatgccggc atcgacctcg tggtgatggg
    28141 tgcctacggc cactcgcggc tgcgcgagct ggtgctgggc ggcgccaccc agagcctgct
    28201 cgatcacatg ccgatcccgg tgctcatggc gcactgaagg ggccggcccg gcggggtctg
    28261 ccgagcctca gcgcggcgcc ggcgggggcg gggcgggggc cacgtcgaga tgcaccgtct
    28321 gcagggcgtc gaggcgggct cgggtctccc tgagctcctc ctcgacgccc ttgaggcgct
    28381 cgagctcggc catgagctcg gcggtgtcgg agaccacgcc atccgtcggt cggagctcgg
    28441 cggcgagccg cgccatggtc cgcagctcct cctcgagccc ggcgatgcgc tggttcgcgg
    28501 cggcataggc ctcgggcgtg atcgcgcccg agctcgtcgt ctgcgcctcg agccggcgct
    28561 ccagctccgc atttcgggca gcgagctcgg cgagctcggc atcgtgcgcc gcggtcgcgg
    28621 cgtcgagcgc ggctgcgctc gccctggcca tgctgtcgcg ctcggccctg aggccggcgg
    28681 cctcgcgccc ggcgagcgcc cgcgcatccc gggcctcggc cagctcggcg gcgagcgtgc
    28741 cggcccgctc cgccgccgtg gcaccggccg cggccgccgt ccgcagctcg gcgagctgcg
    28801 ccgtagccgc ctcgccagcc atccgggcgg cggcgacatc cgctcgcaac gcatcccgct
    28861 cggcccgcac cgccgccagc tcgctcgcga ccgccgcgtc tgccgcggcc gccacatcga
    28921 cctccgcggc ttgtgccgca cgtgccgtgg cgagctcggc cgccagcgcg tcgcgctccg
    28981 cgaccagcgc ggtcgtccgg gtctcggcct cggccgtctg ggcctcgagc cgggtgagcc
    29041 ggtcggccag ctcggacagc agctcctggc cgcggccgag ctccgaggcc agccggtcgc
    29101 gctcggcgac cgcggcggcg gcggccccga gctccgcctg gagcgcgtca cgctcctcga
    29161 ccagcacctg cgcccggctc tcggtttgca gattttcggc gcgcagggcg tcggcctcgg
    29221 ccgtcgcggc cgccagttgc tgcgtcagcc cggcacgctc ggccacggca ctggcgagct
    29281 cgcgggcgag ccgggcgttc cccgcctcga gatcggcgcg ccgcgcctcg gcgctcgtca
    29341 gctccgccgc cagatcgccg gcccgcgtct cgagcgtggc cagagcttcg gcctgctgcg
    29401 cccggccgcg ctccagcgtc gcctcgagca cggccttctc ggcggcgagg tccctgttct
    29461 gcgcctcgag ctgatcgagg cgagccgccg atgccggatc gccggtcgcg gccgggccgg
    29521 cggcacggac cgggccggcc acctcgagcg tggccatgcg ctgcagggcc tggtcgagct
    29581 gcagccgggc cgcatcccgc tcgcgctcga ccagccgcag ctccatgccg agctgggtga
    29641 gctggtcggg agtcacggcc tcgggcgcga ccatcggctg gccggccgaa tcgatcatcg
    29701 cctcgatggc gccgccgacg ccgagctcgg tctcgagcgc cgtgatccgc gagtcggccg
    29761 cagccagcct ggtctcggca tcggcccgcg cctcctcggc atgggcgagc ttgagctgca
    29821 ccgccgccag ctcacgcgcc gcctgctcgc tcgtggcggt gctccggccg gtgaccgccg
    29881 cgagctcgct ctccaggcgt gccacctcgt cggccagggc ctccatcgag gcgccggccg
    29941 tgccgtcgag ggcgccggcc gcgggcgcga cgccgcccct gccgaccgcc tcggccacaa
    30001 ccgggccgcc cgaccgctcg agcgaggcga ggcgcgcgcc ctgctgctgg tagagccggt
    30061 agccatagcc gagcgccgcg atcagggcga tgcagagggc gccgagcaac agcgggaggg
    30121 ccgtccggcc gcgcctgttg ccatgcaagg caacagggtc gtggcgaccc ggatcatccc
    30181 tgaaacgcca tccgttctgc gcgcgggcat cgggcatgct gccgggctct cctcgaacga
    30241 tcctttcttg gcgtcggcgc atcctgaacc cgtgcccgac cgccttggcc gggcaccggc
    30301 acggcggccc gcgagccacc atcgccgtat cgcgccgaga cactgcaagc gcgcgccagc
    30361 aacgtccaat gaaatcgcgc gccaagctcg tttgttaacg ggccggctaa taagcgccaa
    30421 gacaattcag ctcgtgcgtg cgcccagcgg ctcgcggacg ggcagcgcct cggctggcac
    30481 ctcggcagca ggacgcgccg ggagggccag cgcgcgccgc aacagctccg ccagcagccc
    30541 gcaacccgcc tcctcggtga ccagctcgac cgccggcagg ccgcctggct ggccgccgag
    30601 gagaccgagc gccgcgagag cgagcaggtt gccgtgccgg gccggctcgt cggcgcagcg
    30661 caggccttcc tcgttggtcg gggtcgggcc gtcgaacagc accgtgttgc gggccgagac
    30721 gccacgccag aggccgcgct cctgcccggc cacgatgtca gcgaggctgg ccgaatgacg
    30781 cgccgcggcc agctcgtggc ggagcgcctc gggccgcagc gccacgcggt gcgtgaggct
    30841 gcccagctcc ggcagggtga cggtgctgac gaggctcggc tgctcggccg gcgccagccg
    30901 agcccaggcc gtgccccgcc ggacctcgac ggcatcgacg atgcacgggc ggctggcgca
    30961 gagcacgtcc tggctcgcga caccggcgca ttcgatcagg aagacgaagg gctgggcgct
    31021 gccgtccatg gccggcactt ccggcccgtt gagctcgatc agcgcgttgt ggatcccggt
    31081 cagcgccagg gcagccagca catgctcgac cgaggcgatg cggctgccgt cctcgccgac
    31141 gaggacgaag gcgtgctcgc tcggcaccag atgccgggcg gcggccggca ccggcagggc
    31201 gccggcccga tcggtgcggc gaaaggaaat cccgctgccg gccggggccg gatgcagggt
    31261 cagcgccacc cgcgcgccgg aatgccggcc gacaccgaca cagccgatgg gatggcgcag
    31321 cgtgcgctgg acggcaggcg cgggctcgct cgccagcacc accatccggt cgtctcgatt
    31381 cttgctcatg gccccgctcc tcgctggtcc cggtcacgcc gttgcttcgg cgtcgaccag
    31441 tgctggccag gcggcgctcg tccctcgaaa ccgccctttt tcgagagtgc ggacggcact
    31501 caccaaaaaa ggttggtttc cccgccttcc ggcaaccgta ccagaaaaac ggggaaacca
    31561 aatagttatt ttaaactgcc tgtttaggag gagagatcaa agctacctct caacaggcgg
    31621 tgttttagct ctcaacgcgc tccttgcgca agaacgccgg gaagtcaccg agctcgtcct
    31681 ggctcggcgt agcgtttctg ccacgctcct gagcgccctc ggccggtgcc ggcgccttgt
    31741 aggcaggctc ggccggacca tgcgccggcg ccagcgccgg ctcgcgccgg aacggcgagg
    31801 gtgccagcgg ctggccattg cccgtctcgg ggccgtggcc ggtgaagccg cgcgggaacg
    31861 cgattccctt gacccgctcg aagatgcccc ggccgatcga ccggtcctcc tggcgcgggc
    31921 cgacccgccg ggccacggcg tcgtagccgt cgttcggctg ctcgaccctg accggcgcgc
    31981 cctgcgccgg cggcggcacg aagctcggat actggctgtc gccggccggc gccgcctcgc
    32041 gccggccctc ggcggccgcc cgaagctgca gggtggggtt ggcctccgga gcccgctgcg
    32101 ccggcttctg caccgccggg tagggctgcg gccgataggg agcctgcggg aaccagccac
    32161 ccgcctggcc ctggccctgg gcttcgtgcg tcgcgctgct ctcggcggcc gcctgcttgg
    32221 cgcgctcggc cggcgtctcg atgccggtgg cgacgacgga gacccgcagc gcgccctgca
    32281 gcgattcgtc gaaggtcgag ccgaagatga tgttggcctc ggggtccacc tcctcgcgga
    32341 tccggttggc cgcggcgtcg atctcgaaga gcgtgatgtc ggcgccgccg gtgatgttga
    32401 tgagcacgcc acgcgcgccg cgcatcgaca cctcgtcgag cagcgggttg gagatcgcgg
    32461 cctcggcggc ctcgatcgcc cggcgctcgc cctcggcctc gccggtgccc atcatcgcct
    32521 tgcccatctc ggacatgacg gtgcggatgt cggcgaaatc gagattgatc atgcccggca
    32581 tgaccatgag gtcggtgacc ccgcggacac ccatgtgcag cacctggtcg gccatctgga
    32641 aggcatcggc gaaggtcgtc cgctcgttgg ccagccggaa gaggttctgg ttcgggatga
    32701 cgatcagcgt gtcgacgaac tgctgcagct cggtgagccc gccctcggcg aggcgcatgc
    32761 ggtgggcacc ctcgaagtgg aagggcttgg tgaccacgcc gacggtcagg atgcccatgt
    32821 cgcgcaccgc ccgggcgatg acgggtgccg cacccgtgcc ggtgccgccg cccatgccgg
    32881 cggtgatgaa caccatgttc gagccctgca ggagatcgag gatctcgtcc aggctctcct
    32941 cggccgaggc ccggccgacc tcgggccgcg caccggcgcc caggccatgg gtgatggcgt
    33001 ggccgagctg gatcttgcga tcggtcaggc agttggccag cgcctgcgcg tcggtgttgc
    33061 aggcgacgaa ctcgaccccc tcgagatgcg aggtgatcat gttgttgacg gcgttgccgc
    33121 cggcgccacc gacgccgacc accgtgatgc gcggcttcag ctcatgcgtc accggcatgc
    33181 tcaagctaat cgtcatattc tcctccttcg ttgtgtgcgg tcgccgaccg ttttcggttc
    33241 gtgcgccgcg ggcagtggcc gtaccctggg cagggggcag ccgggaaaag ggtcttcttc
    33301 gctgggtccc ggttctccaa gacctcagct cacgaagttc tgcttgagcc agtgaccgag
    33361 cctggccagg ccattggtca gcgccgggac ctccgtgctc tgcttccagc cgagcccgcc
    33421 gtcccggccg gtgaccagca ccagcccgcc ggcggcggcg ctgcagcagg gctgatcctc
    33481 gacgccggcg gggccgcgca cggcacccgg ccggccgatc cgcgccggca ggccgaacag
    33541 ctcctcggcg agctcgacga tgccttccat ctgtgccgca ccgccggtca gcaccatgct
    33601 gcgcggcggc cgtgactgga ccagctccca gtcgtccttg agccgttcct ggaccagccc
    33661 cagggtctcc tcgacccggg cccggatgat ctgcgtcagc cgcgtgcgcg gcacctcgcc
    33721 ggtcggctgc tcgagctgat cgccgatcag cggcacctcg acccgcacgt tgtcgtcgca
    33781 ggcgcgccac tgcaccccgc catagaggtt cttcagccgc tcggcgaaga cccggctggt
    33841 cttcagcccg taggccagat cgcgggtcac gtgctcgccg ccatagggca cctggccgag
    33901 gtggcagagg gcgccgccct ggaagagcgc atggctggtg ctgccgccgc cgagctcgac
    33961 caggagcacg ccccgccgca tctcgtcgag ggtcaggcag ccgaccccgg cggcataggc
    34021 accgttcgcc acctccatga tgtcgagatg gcagcgctcg aggcaggccg tgagattgcg
    34081 cagggccggc ttggcgacgc tcaccacgag cccggtgaac tccagcacct ggccggcgag
    34141 gccgcgcggg tcggccagcg gccggccgcc gtcgaggcgc atctcgaccg gcaggatgtg
    34201 caggatctcg cggtcgctgc cgaccatggt ggcgcgcacc gattcgaggg cccggtcgag
    34261 atcgtcgtcg tcgatcacgc ggccgccgag atcgcgctcg atgctgacga gaccggcgcg
    34321 gggcgcgccg ccggaccagg tcacggcgat ctcgcgcagc gtctcgcccg ccgcctcctc
    34381 ggcctcgtgc agcacggcca gcatcgaggt ctcggccgcc tcggcgtcgc tcacctcgcc
    34441 ggcctggaag ccgtcggccg actggtagcc gcgaccgagg agctggaagc cgccgctgct
    34501 ctgcgtgcgg acgacgtagc agcagagctt ggagctgccc acatcgagca ggccgaacgg
    34561 ggcattgcgc gccgtgcggc gctggctctg cagcaggcgc tgccaggcca tctctgcctt
    34621 ggcgttcatg tctgttgtcc ccgtgtggca tgggccgcgg attgcaggat cgcctcgtcg
    34681 aggcggacga cgacccggtc cggcaggcgc aggtcgaccg cctccaccgc ccgggcgagc
    34741 agcgccgatc gggcctcgag ccgggccagc cgcgcccagg cggcggcggc accgcgctcc
    34801 ggcaggcgga cctcgatgcg accgtcgacg aagacgttcc agcgccggcc gtcgacccgc
    34861 tgggcaccgc tgacccgttc gccgagcgac ggcacgctgg ccaccagccg catcagctcg
    34921 ctcgcccccg cgggtgcgcc ggcaccgctg aggatcggca gatggcggta gggcgagagg
    34981 gacctgacct ggatgatgtc accctcgacg ctgaccagcg ccggcccgtt cgccgtctgc
    35041 cagagggcga tcggccgatg ctcgaccaga cgggtccgaa gctggtcggg aagctggcgg
    35101 gtgacggttg cattctggac ccatggcagg ctctccagac gcttctgcag ggcggcaata
    35161 tcaaccgaaa gtatgttttg tccataatag ggttctagta tttggacaat ttcggcggct
    35221 gtggtgcgct ggcgaccctc ggtgaagatc tcgcgcacct gcatgcccac ggcatcggtc
    35281 acggccacca gctggtcgtt cagcgcgaag cgcgcccggt cggtcaggcc gctggcccgc
    35341 gccagatgca gggaaaggcc gagcacggcg gcgcagccga cggcgacgag gccgttgatc
    35401 aggggctcgt gccagcgcgg cttcggcggc tggcgcagca cgtgccgggt gcggtaggcg
    35461 agctgcctca tctgtcgcat cgggccctct cgatcagccg gacgaccagc tcgccgaagc
    35521 cgatgccgcg atgcctggcc tgctccggca ccagcgacaa aggcgtcatg ccgggctggg
    35581 tgttgacctc gagcagcacg agcccgtcca tgcccttcga cgggtcgaag cggaaatcgg
    35641 cgcggctgac gccgcgacag ccgagcaccc agtgcgcgcg cagggaccag tcgcagacct
    35701 tgtcgaggac cggcctgggc aagggggccg ggacgatatg gtcggcgaca ccttcggtgt
    35761 acttggcgcg gtagtcgtag aagccgccct tcttcttcgg ccggatctcg gtgacggtca
    35821 gcgcctcctc gccgagcacg gcgcaggtca gctcgtggcc cggcacgtag cgctcgacca
    35881 agaagcgcgt gtccggggcg atgtcctcgc ggtcgaccag gccgccgatg tcgcccttca
    35941 tggcgatcac cacgtcgacg ctcgagccct cggccgccgg cttgacgacg aagggcggct
    36001 ccatcggcgg cggcccgtcc tgcagatcct cgaactcgat ctcgaagccc tcggggcaga
    36061 cgaggccggc gctgcggaag agccgcatgg ccatcggctt gtccatggcg agggccgagg
    36121 cgagcacgcc ggaatgggta taggggatgc ccatcaggtc gagcacgccc tgcacccgcc
    36181 cgtcctcgcc catgcggccg tgcagcgcgt tgaacaccac gtccggcttg agctcggcca
    36241 gcaccatggg caggtcgcgg ccgacatcca cccgggtcac tcggtagccc tgcccctcga
    36301 gggcggcggc gcagttctcg cccgagacga gcgagacctc gcgctcggcg gacaggccgc
    36361 ccatgaggac ggcgacgtga cggttcatcg atgctctcca acggtttgcc gcggctcggc
    36421 cggcaccaca tccggcgacg cctcgcccaa ccgcttgatt tcccattcca ggcgaatgcc
    36481 gctctgggcc gccacccggt cccgcacgat ctcccccagg gcctcgagct cggccgccgt
    36541 ggcaccgccg gtgttgatga ggaagttgca gtgcttctcc gacaccatgg cggcaccgcg
    36601 ccgcaggccg cggcagccgg ccgcgtcgac gagctgccag gcgcgctgcc cgggcgggtt
    36661 cttgaacgtg ctgccaccag tggcgacgcg gatcggctgc accgcctcgc gctcggccct
    36721 gatcgccgcc atgcgctcgc cgatcgcccc aggctcgccc ggctggaggc cgaaggcggc
    36781 ccgcaccacg atccagtcct ccggcagcgc gctcttgcga taggcgaagc ccagctcggc
    36841 attggtcagc cggtgcagcc tcccctgccg gtccagcgcc tcggcccaga gcaggcggtc
    36901 cctggtctcg ccgccgaagg cgccggcgtt catccggacc gccccgccga tcgtgccggg
    36961 aatgccgatc atgaactcga agccggcgag cccggcgcgc tcggctgcct gcgccgcctt
    37021 ctgatcgagg gcgccggcgc cggcgatcag cgtggtgccg tcgatctcga tccgggcgag
    37081 cgggccgccg aagcggacca cgaccccgcc gatgccgccg tcgcggatca gcatgttgga
    37141 ggccacaccc atggccagca ccggcacctc ggccggcgtg gcggcgagga aggaagcgag
    37201 gtcgtccgcg tcggccggct ggaagagccg ggccgccggg ccacccaccc taaaccaggt
    37261 gagcggggcg agcgtcacct gctcgcgcat ggtgccgcgc acggcttctc gacgggccgt
    37321 catgcccgca gctcccgcag gcgctcgggc agcgcattgg cccactgggt gatgctgccg
    37381 gccccgaggc agacgacgag gtcgcccggc tgcgccagct cggcgatgcg cgccggcagc
    37441 tcgtccggcc cgtcgatcgc ggcgacgtcg cgctggccgt ggccgatcag cccggccacg
    37501 agcgccgcgt ggtcgacgcc cggccgcggc gtttcgcccg cggcatagac cggggcgacc
    37561 agcacgctgt cggcattggc gaagcaggtg cagaaggcct cgaacagctc ctcgaggcgg
    37621 ctgtagcggt gcggctggac gaccgcgatc acccggcccc cggtcgcctg ccgcgccgtc
    37681 ttgagcacgg cgcggatctc gaccgggtgg tggccgtaat cgtcgacgat ggtgatgccg
    37741 tccacctcgc cggtcaccgt gaaccgccgc ttgaccccgc cgagccccgt cagcgccttg
    37801 cgcaccacgt cctcgccgaa gccgagctgg tcggcgatgg cgatggccgc caaggcgttc
    37861 tgcacgttgt ggtagccggg cagcgccagg tggagatcga cgaagcggcg ctgctcgccg
    37921 gtggcaggat cgcgaacgaa gacgtcgaag gtcgagccct tggcgttgga gatcggctcg
    37981 gtcgcccgga tgtcggcctg cgcgctgaag ccgtaggtgc ggatctgccg gtcgccgatc
    38041 cggccgatca gcgcctggac ctcggggtgg tcgatgcaga gcacggcgag gccgtagaac
    38101 ggcagccgct cgatgaagtc gcgaaagccg tccctgaccg cgtcgaagct gccccagaac
    38161 tccatgtgct cggggtcgat attggtgacg atgccgatgg tcgcgggcag gcgcaggaag
    38221 ctgccgtcgc tctcgtcggc ctcgaccacc atccactcgc cctggccgag ccgcgcattg
    38281 gtgccgtagg cgttgatgat gccgccattg atcacggtcg gctcgagccc ggcggcatcg
    38341 aagagggcgg cgacgatcgc cgtcgtcgtc gtcttgccgt gggtgccggc gaccgcgacc
    38401 gagggatgca ggcgcatgat ctcgccgagc atgtcggcgc ggcggacgat cggcagccgc
    38461 cggcggcgcg cctcgaccag ctccgggttg tcggccttga tcgcggacga caccaccagc
    38521 acccgggcgt cgcccaggtt ctccgcccca tggccgatgg cgaccgccat gcccatgtcg
    38581 cgcagccgct ggacattggc gctctcggcc acgtccgagc cctgcaccgc gtagcccatg
    38641 ccgtgcagga tgtcggcgat gccgctcatg ccgatgccgc cgatgccgac gaaatggatg
    38701 acgccgcaat cgagcggctg gatgcgcagg ctgcggaccg ggctcaaggg ctctctcccg
    38761 ttgccggaac cagcgccagc acggcatcgg cgagccgccc cgcggcatcg cggcggaacg
    38821 tggcgagcgc cgccgtggcc atggcctcga gccgctccgg tgccgccgcc agggccgtga
    38881 gctcggccgc cagcgccacc ggggtgaagg acgcctggag catgcgcacc gcaccgccgg
    38941 cccgggccag cgcctcggca ttggcgcgct ggtggtcgtc ggcggcggcg gcatagggta
    39001 cgaggatcga aggccggccc atggccatga tctcggtcag gctggaagcc ccggcccggc
    39061 tgatgacgag atgcgcccgg gccagatgcg ccggcatgtc ggtgaagaag ccggcgacct
    39121 ccgccgcgat gccgtgcgcc ttgtaggcgt cggccacccg ggccaggtcc tccggccggc
    39181 actgctgctc gatgcgcagg cgacgggaca gctccgccgg caggagggcg accgccgccg
    39241 gcaccacgtc gctgaagatc ctggccccct ggctgccgcc gatgaccagg atgcggatcg
    39301 gctcgttcgg gccgggggca cgatagccgg ccgggtccgc ctcgaagccc ggccgcaccg
    39361 ggttgccggt gacgacgagc cgggcccggc cgcgctcggg cacggcgctg gtcgcctcga
    39421 agctgagggc gatcacctcg gcgaaccgtg ccagcagccg gttggcccgg ccgaagacgg
    39481 cgttctgctc gtgcaggaga agcggcctgc cggcgagcct tgccgcgatc gcggccggca
    39541 cggatgcata gccgccgaag gaggccgcgg ccctgggctt gagccggcgg gccacgcgcc
    39601 aggcgtcgaa gatgccggcg acgagggcgc aaattccggc gaggcgcctg ggcaggctgc
    39661 ccgtcgggct gccggcgcga accagatggg cctcgaggtc ctccggcatg aagcgggcgc
    39721 cacgcctgtc ggtgaccatg gcgaccggct cgccccggcc gcgcagctcc cgtgccagcg
    39781 ccagagccgg gaacatgtgg ccgccggtgc cgccggcggt gaccaggatc ggccccctca
    39841 tggcacgggc tccagccggg cgccgcgccg ggtgagggcg agcagcatgc ccatggtcag
    39901 cgccaccgcg agcagcgacg agccgccata ggagatgaac ggcagggtca tgcccttggt
    39961 cggcaccagg ttgaggttga ccgccatgtt gatcagcgcc tgcaggccga actgcgcgac
    40021 gaggccggtg gcggcgagct ggacgaagcg gttggggttg agctgcgtgc gccagagggc
    40081 gcgcagcacg agggcgcaga agacggcgac caggacgagg cagatgatga tgccgaactc
    40141 ctcggcgacg gtggcgaaga cgaaatccga atgcgcctcc ggcaggcgga acttcaccac
    40201 cccctcgccc ggcccccggc cgaagagccc gcccgactgc acggcgctca gcgcccgctc
    40261 gacctggtag acctcggccg tcggatcgag gaacccgtcc acccggtcgc ggacatgcgg
    40321 caggagctgg taggcgcccc agagcccggc cacgccggcg ccgccgagga tgatgatcgg
    40381 cagccagccg agcccggcga cgaagagctg gatcgagaag aaggcgacga ccaccgtcgc
    40441 catgccgaga tccggctgca gcagcaggag cgccacggtc ggcccgacca gcaggagcgc
    40501 cgccggcagc ccgccgagac cgggccggct ggcgagcagc caggcggtga cgacggccag
    40561 ggccggcttc atgaactccg acggctggag ctgctggccg ccgatgaaga gccagcgatg
    40621 cgcccccttg atctccggcg cgagccagag cgtggccagc gtcagcacgg cgaagagggc
    40681 cagcacgccg acggcgagcc tgagcacgcc ccgcggcgcc agcagcgaga cgccgaagag
    40741 gccgagcgcc gtcggcacca gcacggtgaa gtgcttgatg acgaaatgca tctggtcgag
    40801 cttcaggttg cgcgcgatgg gcgggctcga cgccagcacc atgatgatgc cgaagaggat
    40861 cagaaggccg atgccgatca gcaggaaccg gtcgacggtc caccaccatt ggccgagcag
    40921 cgagcgatcg gtgcgcgcga aggcgttcat gccgcgacgc cctcggcaag ctcgcgggcc
    40981 agcgtcgcga aggccgcgcc acgcgcctcg tagctggtga actggtcgaa ggaggcgcag
    41041 gccggggcca gcagcaccac gggctccggc gcgttgctgg tgcgcgccgc ggcgaaggct
    41101 tccggcacgg cgacatcgag ccggcccgcc aggtgccgcg gcatcgcggc cggcaaggtg
    41161 gccgcgatct cgcccgccgc ctcgccgatc agccaggcgc cgcgcaccgc cgccagatgc
    41221 ggcaggaggg cggcgaagcc gccgggcttg ggcttgccgc cggcgatcca gaagatctcg
    41281 gcgaagctcg agaggctcgg gatcgcggcc tccgggttgg tcgccttgct gtcgtcgacg
    41341 aagcgcacgg ccccggcccg cgccacggtc tgcatccggt gcggcaggct ccggaagctg
    41401 gcgaagcccc cgaccgccgc ctcggccgca acgccgagcg ctcgcagcac ggcccaggcc
    41461 gccgccgcat tctgccggtt gtgccggccc tcgagggact cgatgccggc gagatcggcg
    41521 accgtccgcg gcgtctcgcc ggtgccgtcg accagcagcg tcccctcggc ccagacaccg
    41581 cccggcaccg gccccagggc gctgatccgg atcaccttgc gccccgcggc cgcgagggcg
    41641 tcggccagcg cccgggacgg cgcgtcgtcg acgccgacga cggcgacgcc gccttccggc
    41701 tgcagctcga agaggcgccg cttcgccgcc acataggcct cgaacgtgcc gtgccggtcg
    41761 agatggtcgg gtgtcaggtt gagcagcacg gcgacctggc aggcgagctt cagggccagg
    41821 tcgagctggt aggaggagag ctccaggacg aagacccgcc ccgatccgcc gagctcgagg
    41881 tcgagcaccg gccggccgat attgccgccg agctccgcat cccggccggc cgccaccagc
    41941 aggtgatggg ccagcgcggt cgtcgtcgac ttcccgttcg tcccggtgat gccgaccacc
    42001 gtccgcccgg ccagccgctc ggcgaagagg tcgatgtcgc tggtgagcgg cacgccggcc
    42061 gccctcgccg ccgcgatcac cgcgtgcggt gccgggaaat aaagcggcac gcccgggctg
    42121 acgacgaggg cgtcgagccc ggcgacgtcg ccgaccttgc ccgcccggcc gccttcggcc
    42181 acggcggcgg ccacgctttc cggccggtcg tcgtagacga ggcagtcgat gccccgggcc
    42241 agcgcgtccc gcagcaccga gcgcccgctc cgcgccatgc cgagaatgcc gatgcgcccc
    42301 ttcccgccca tcgccgtcat cgcagcttca gcgtcgaaag gccgatcatc gcgaggatgg
    42361 tggcgatgat ccagaagcgg atgacgatcg tgggctccag ccagcccttc ttctcgaaat
    42421 ggtgatgcag cggcgccatg cggaagaccc gccggccggt cagcttgaac gacacgacct
    42481 ggacgatcac cgagaccgcc tcgagcacga agagcccgcc gatcaccgcc agcaccagct
    42541 cgtgcttggt ggcgaccgcg atggtgccga gcgcaccccc tgtggcgagc gagccggtat
    42601 cgcccatgaa gaccatggcc ggcggcgcgt tgaaccagag aaagcccagg gacgcgccga
    42661 ccatggcggc gcagaagacg gcgagctcgc ccgtgccggg cacgaagggg atgccgagat
    42721 agcccgagaa gatggcgttg ccgacgagat agccgatcag cgcgaagcag ccggcggcga
    42781 tcatcaccgg cacgatggcg agcccgtcca gcccgtcggt gaggttcacg gcgttcgagg
    42841 cgccgacgat caccagcatg ccgaagagcg ggaagaagag cccgagcggg acgaggagat
    42901 ccttgaagaa cggcagcgcc agcgaggtgt cgagcggctc gggcgtcagc cggacgagga
    42961 ccaccgccgc caccagggcg atcgtcccct gcaggaagag cttcatccgg ccgctgatgc
    43021 cggccgacga gcgcctggtc agcttcatca ggtcgtcggc gaagccgatc ccgccgaagc
    43081 cgagcgtcac caccagcacg acccagacat agccgttggc gagatcggcc cagagcaggg
    43141 tcgagacggc gatcgcgatc aggatgagca tgccgcccat ggtgggcgtg cccttcttgg
    43201 tcaaaagatg cgattccggc ccgtcctcgc ggatcggctg gccctcgcgc tgcttttgct
    43261 tgagccagcg gatgaccggc gggccgagga tgaagctgat caggagcgcg gtcatcgccg
    43321 ccccgccgct ccggaacgtg atgtagcgga agacgttgag caccgagatc tcgtcggcga
    43381 agggcaggag cagatagagg atcaccggcc ctggccctcc accggcgtcg cggacgggtt
    43441 ggccgtcctg tcgagcgcct cgaccacgcg ccgcatggcg ctgccgagcg agcccttgac
    43501 cagcaccacg tcgccgggct cgagggccgc gacgaccagc ggtgccagcg cctcgctgtc
    43561 cgccgcccag gcgccgcgcc gggacgcggg cagggcatcg aagagctcct gcatcagggg
    43621 ccccgccaga aagaccttgc tcgcatccgc cgcgagcacc gcgtgcgcca gcccggcatg
    43681 cagcaggccg gcaccggcac ccagctcgag catgtcgccg agcaccgcga tccgccgccc
    43741 cgcctggctg cccagcacgt cgagcgctgc cgtcaccgag gcgggattgg cgttgtagct
    43801 ctcgtcgagc aggcggatcc ggccgccctg ccagggcagc aggcgctggc cgccgcggcc
    43861 ggccagcggc cgcacctcgc ccagccgcgc cgccgcgtgc ggcagatcga ggccgagaca
    43921 gtcggcgacg gcgagcacgg ccaggctgtt catcgcccaa tgccggcccg gcgctgccag
    43981 cgccagctcg agccggcgcc cgccgagggc gaccgtgagc cggctgccgc cggcctccag
    44041 atcgagggcc acgagccgca cgtcggcctc gccgacggcg ccgaacagga ccaccctcgc
    44101 cgcccgcgca cgggccgcct cgaccagcag gtcggcatgc ggcgcgtcgg cattgaggat
    44161 cgccgtgccg ccgggctcga gcccttcgaa gatctccgcc ttggcccggg cgatcgccgc
    44221 cgtgtcctcg aagaaggcca gatgtgccgg ggcgatcgcg gtgatcaccg cgacatgcgg
    44281 ccggaccatg gcggtcagcg ccctgatctc gcccgcatgg ttcatgccga gctcggccac
    44341 ggcgaagtcg gcctcgggcg gcaggttggc gagggtgagc ggcacccccc agtggttgtt
    44401 gtggctggcg gcgctggcat ggacccggcc gaagccgccg agcacgtggc ggagcgcttc
    44461 cttcgtgccg gtcttgccga cgctgcccgt gatgccgacg accctggccc ggctgcgcgc
    44521 ccgcccgaac cggccgaggc cctcgagcgc gctctgcgtg tcgtcgacga cgagcaaggg
    44581 cgcatcctcg tcgacatcct ccggccgccg cgtcaccacg gcggcggcgg caccctgacg
    44641 caaggcatgg gcgacgaagc cgtggccgtc ccggctgggg ccggcaaggg cgacgaagag
    44701 atcgccttcg ccgacgctcc ggctgtcgat ggagaccccg gcggcatgcc agtcgcggcg
    44761 gctccggccg ccggtcgcgg cggcggcatc caccgcctcc cacaagggct tcagcgtgac
    44821 catgctcatg ccgcgctctc cagcccggcg agcgccgtcc tgagctcggt cgcatcgtcg
    44881 aagggcaggg tctcctcgcc gacgatctgg ccctgctcgt ggcccttgcc ggcgacgatc
    44941 aggacgtcgc cgggctcgag cgccgcgaag gcggccctga tcgccgccgc ccggtcgccg
    45001 atctcggtga ccttgccggc ccggccggcg agcggctcca ggatctgccg gcggatcgcc
    45061 gccgggtcct cgttcctcgg gttgtcgtcg gtgacccaga cccggtcggc ccgctcgacc
    45121 gcgacctcgc ccatcagtgg ccgcttgccc ttgtcgcggt tgccgccgca gccgaagacg
    45181 acgtgcagcc gccccttggt gtgcggccgc agcgcgtcga gcgccttggc cagcgcatcc
    45241 ggggtatgag cgtagtcgac gaaggccggc gctccggacg ggtggcgcac gacgagctgc
    45301 atccggccgg gggcaccgcg cagctcggcc agggccagga tcgcctgctc gagcggcgcc
    45361 ccgccggcca ccgcgaggcc gagggccgcc agcacgttgt gcgcctggaa ggcgccgacc
    45421 agccgggtgc gcaggcgatg cgtccggccg tgcacggcga tctcgagcac ctggcccgtc
    45481 agctcgggct tgcggccgag cagcttgagg tcctcgccgg cctcgccgaa ggcgacgacc
    45541 gggcagccgc gcttgttgca gatcttgacc agggtcggga actcggggct gtcggcgttg
    45601 aggacggccg tgccgcccgg caccaggcac tcggtgaaca gccgccgctt cgccatgaag
    45661 tagtcgtgat agctgccgtg atagtcgaaa tggtcgcggc tcatgttcga gaaggcggcg
    45721 gcatggatgt cgagcccggc gagacgctgc tggtcgaggc cgtggctcga cgcctcgacg
    45781 accagatggc cgaccccgcc ccgcaccagc tcggccgcga cccgatgcag ggtcaccgcg
    45841 tccggcgtgg tcagggagac gctgggccag cggccgcggc cctcgaggcc gagcgtgccg
    45901 aggctggcgg cgcggatgcc gagccggtcc cagatctgcc gggtgaagct ggcgatcgag
    45961 gtcttgccgt tggtgccggt gacgccggcg gtgaaggccg gctgccccgg gtagaaggcg
    46021 gcggcgaggg tggcgaggcg gcgcctgggg tccgggtcga tgatcaccgg cacgagctgg
    46081 gcgtaggcct cggcggcggt cgtgccgagc accgccacgg caccccgctg gacggcctcc
    46141 gggatgaaga tgtggccatc ggccaccgtc cccgaaaggg cggcgaagag gttgcccggc
    46201 tcgaccttgc ggctgtcgaa ggcgatgccg gtgatggtca ccggcgcgag attcaggagg
    46261 cggccggcag ccgccggctt gcgcccccgc gccgcctcgc gccgcgcttc ctcagcgagt
    46321 aaggctggca gccgcaaagc cgtcctccat tcgccgggtc cgtccgttca tgaccggcac
    46381 cacctgcagc cggtcgcgca gcgccagctc gctctccggc tgggcccggg gcacgccgag
    46441 cagcggcccc gcctcggcaa tgatccgggc gaccaccggg gccgcggtcc agccgccggt
    46501 gcggtagccg taggtcttgg cgtcgccctt cggctcgtcg agcatggtca gcaccgcgaa
    46561 gcgcggcgca tcgatgggaa agacgccgac cagggaagcg agcaccgcct gcccgtcata
    46621 gccgcgccgg ccggcggtcg gcttgtcggc ggtgccggtc ttgccgccga tcaggtagcc
    46681 cggcacggcc gcatgggtgg cggtgccgcc atcgacggtc agccacatga gccagcgcac
    46741 gtcacgggcc gtggcggccg acaccacggt cgggccatcg atcgccgcat cggccggccg
    46801 ctcgagcagg gtcgccggca cgcgccggcc gtcgcccgcc aatgtcgcga cggcctcgac
    46861 gaactgcagc ggcgagacgg cgacgccgtg gccgtaggag acggtggcgg tgacgatgtc
    46921 cggccaacgc tcgggcacga gcggggcggt cgtctccggg atctcgagca ccggccggtc
    46981 gagcaggccg agcgccttca ggaaggcgcg ctgcgcctct cccccgccgg cggcgaaggt
    47041 catccgggcg gtgccgatgt tcgaggagaa ggcgaagacc tcggggaccg tgagccagcg
    47101 gttgtggccg tgatagtcgc gcacccggtg ccggccgatc tgcaggggct cggaggcgtc
    47161 gtaccggtcg tggagcgaga cggcgccgct ctcgagcgcc atgccggcgc tcaccacctt
    47221 gaacagcgag cccagctcgt agacgctgct ggtgcagcga ttgcggcgct gctccggcga
    47281 ggcctgctcc ggccggttcg ggtcgaaatc cggcaggctg accagcgcca ccacctcgcg
    47341 ggtcgccatg tcgagcagga tggtgcaagc cccttccgcc cggaaccgct gcaggccggc
    47401 cgaggtcgcg gcgtgggcga tacgctggat gcgcaggtcg atggacagcc gcacgggcgg
    47461 cttgccgagc tcgctgccgc cgaccagcgc ctcctgcagg ccgtgctcga tgccggcgag
    47521 gccctggttg tcgatgtcga cgaagccgag cacgtggctg acctcgctgc cgcgcgggta
    47581 gacgcggcgc tcgccgagcc ggaactcgac gcccggcagg ccgagatcga gcaccgccgc
    47641 ctgctcgatc ggcgagatct gtcgcttgat ccaggcgaac cgcccgccgc gggcgaagcg
    47701 cgccctcagc tccgtggcgt cgacgccgtc gagcaccctg gcgagggccg tggccacggc
    47761 gtcggggtcg ttcatccgca tgggatcgcc gaccaccgag agggtggcga tgttggtcgc
    47821 gagcagggcc ccgttgcggt cggtgatgtc ggcgcgccgc gggtccggcg cgtcgtcgcc
    47881 gcccggcggg acgaaggcgg cctcggcccg gttgccgccc accatgtcga cgaggcggat
    47941 gccgatgctg acgaaggcga gcacgaagca gagcgccagc acccgcaggc gcgcggtcgt
    48001 cagggcgttg gcgccgcccg cggaccggct caccactcgg ctccgagcct gtcgccgccg
    48061 gccccgagca cgccgagatc gaaggtggcg accggcttga cgcggaactc gatggcctcg
    48121 ccgtccggca gcagcgcgct cctcgcctcg cgcgccagct cgaggttgcg gtagttgccg
    48181 atctgctcgg cctgcaccac gcgggacaac cgcaagggct gcatgtccag ggcccgcgcc
    48241 tgggcggtca gccggtcggc ccgcacgaga taggcccagt ctgcctcgag cgtcttcagc
    48301 cgccagcgct cggcctcgat ctcgcgctcg atccgcacga tctcgctctc ggccgacacg
    48361 accgcgtact tgacgcgaaa gagcccggtc gcggcgacca ggatcagcac cagcagcacc
    48421 ggcacgtagc gcgggatcat gcggccgccc tccaggtctc gaccatcgac ccgccactgg
    48481 cgccgtgcgt caccgcctcg ccctcctcgg cgagcttgac cgccgcccgc aggcgcgccg
    48541 agcgcgcccg ggggttggtc tggacctcgg cctcgccggg ccgcaccacc ttgcgctcga
    48601 gccagcgcag ccgcgcccgc gtcgcctcgg tggtcagcgg cagatggcgc gatggccggg
    48661 cgacgcgacc cgccgcctgg tcgatgaagc gcttgatccg ccggtcctcg aggctgtgga
    48721 aggcgacgag cacgaggcgc ccgcccgggg cgaggcagtc gggcgccgcc tcgagcagcc
    48781 ggtcgagctc gccgagctcg tcgttgacgg cgatcctgag cgcctggaag acctgcgtcg
    48841 ccggcgagcg gccgggcttc ttctgccgct tggcgacggt gaccacggag acgagctcgc
    48901 cggtcgtggt gatcggccgc tccgccagcc ggcggacgat ggcgcgggcg acccggccgg
    48961 cctcgggctc gtcgccgagc tcgcgcagga gccgggcgag ctcggcctcg tcgagccggg
    49021 cgagcagctc ggccgccgtc tcgcccgtgc cgcccatgcg catgtcgagc ggcccgtcgg
    49081 cctggaagga gaagccgcgc gccgcctcgt cgagctggaa ggaggagacg ccgagatcgg
    49141 cgacgatgcc gtcgaccggc ccctcgacca gccccgtgcc ggccgccccg agcaggcggg
    49201 gcagctcgcc gaacgtgccc tcgagcatgg tgaagcgcgg ctcggcagcg gccagcgccc
    49261 ggccgcgggc gacggccgcg gggtcgcggt cgatgccgat cacccggcag gaggcggcgg
    49321 cgaggatcgc ccggctgtag ccgccgccgc cgaacgtggc atcgacgatc gtctcgccgg
    49381 cggcgggtgc gagggtctcc agcatggcat cgagcaggac gggttcatgg gccacggcgc
    49441 tgcccccgga ttcgatccac aggcaggtga cggtgcgcgg ggcggcgcgc cgtccgggaa
    49501 taccggtcac gcagggcgcc gaccgccgcc cggagctggc ccggtgctga agacaaaacg
    49561 ccgatgaaaa ctcgattccg ccgcaccgct ctcgccgtcc gtctgctgtc gccccaagac
    49621 acctgccggc aatcctggcc gaacgcgcat ttctaccaat gcgcgacctc acgctgcaag
    49681 cccctcgtct ccgagcgaga agaaaaacgc gccacgccgt cgaacgagaa cttaactcga
    49741 acagccatcg atttgcacag gcagcgcttt tttggtctgt cggagtgagg gaagactatc
    49801 cgcggtaggg gcctccggtc aacctttcgt tgctgaaaga gcacactgag aacctcttct
    49861 aacaagctga aacgcatcgc taaagggctg tcaagaatat ttctgtggat aagtgccgca
    49921 caattccgcc gggcttgtgg acaagtcctg cacaaatccc tgccgggcgg gtcggcgcat
    49981 acgccgatgg gtagaatgtg ggtgcgggcg gcctataagc cgggttctgt cctcgatcgg
    50041 gatcgagggg tgaccattca tctgggacgt gcgttgccgc acgcctcgcg caacctaccc
    50101 gagcggtcta cccggaaaca gggtcgcacc cgaagatgcc gccgctccta ttcggtcttg
    50161 ctcccggtgg ggtttgccgt gccgtcgccg ttgccggcgc cgcggtgcgc tcttgccgca
    50221 ccctttcacc gtcacccgca gagcggccgg cccgaaggcc gcctctcccg gcgggcggtc
    50281 tgctctctgt tgcactttcc ctggggtcgc ccccgccggg cgttacccgg caccgtgttt
    50341 ccgtgg
//
LOCUS       DAOTFI010000002        48560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_747_length_48560_cov_6.718627, whole genome shotgun sequence.
ACCESSION   DAOTFI010000002 DAOTFI010000000
VERSION     DAOTFI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 48560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48560
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_48560_cov_6.718627"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /gene="rpsB"
                     /locus_tag="P5558_00220"
     CDS             <1..694
                     /gene="rpsB"
                     /locus_tag="P5558_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRY22762.1"
                     /translation="VVAGGGRVLFVGTKRQAQEPVARAAQRCGQYYVNHRWLGGMLTN
                     WQTISNSIRRLRELESRLREQQSGLTKKELLQLSRERNKLEQALGGIKEMGGLPDILV
                     VIDTNKEDIAVTEAKKLGLPVVAFLDSNSSPEHIRYPVPGNDDASRAINLYCDLLVQA
                     VLDGLQAEMTASGMDLGEIEPIEPELAEPELAEQELVHSAPVQPLADAPEETGPEMAP
                     VEDTKASGAAAS"
     gene            740..1666
                     /gene="tsf"
                     /locus_tag="P5558_00225"
     CDS             740..1666
                     /gene="tsf"
                     /locus_tag="P5558_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRY22763.1"
                     /translation="MAEITAALVKELRERTGAGMMDCKKVLIETAGDIEGSIDLLRKK
                     GLAAAAKKAGRTASEGLIGMALEGTEGALVEVNSETDFVARNDAFQALVTAITGEAIA
                     AGGDVETLRAATLPTTGKSVADSLTDAIGVIGENMNLRRAVKVTVPTGVVGGYLHQQV
                     APGLGRIGVLVGLESTGDKAVLAEFGKQLAMHVAAANPQSVAVADLDPAVIERERAIF
                     ADQARESGKPESIIEKMVDGRLRKFYEESVLLEQTWVIDTDKKVKEAVADAAKAAGAP
                     VKVTRFERLALGEGIVKEESDLAAEVAKLTTV"
     gene            1716..2459
                     /gene="pyrH"
                     /locus_tag="P5558_00230"
     CDS             1716..2459
                     /gene="pyrH"
                     /locus_tag="P5558_00230"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRY22764.1"
                     /translation="MSEAETVTPRYRRVLLKMSGEALLGDQSFGHDSKVLRQLASEVK
                     AAHALGVEICMVIGGGNIVRGAALAGAGVERASADYMGMLGTVINALAVQSVIEQAGL
                     ATRVMSAIPMSSICEPYIRRRAIRHLEKGRVVIFAAGTGNPFFTTDTAAALRAAEMGC
                     DVLLKATQVDGVYDADPKRDKTATRFERLSYLDVLARDLRVMDASAISLARESNIPIL
                     VFSVHVPGALARVLRGEGMFTIISGEAAA"
     gene            2456..3019
                     /gene="frr"
                     /locus_tag="P5558_00235"
     CDS             2456..3019
                     /gene="frr"
                     /locus_tag="P5558_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRY22765.1"
                     /translation="MSDGEPDIDDLTRRMDGAVEALRKEFSGLRTGRASASLLEPITV
                     DAYGASMPLNQVGTINVPEPRMISVQVWDKGLVKSVEKAIRNSALGLNPQVDGTLMRI
                     PLPELSEERRGELVKVAHRYAEQARVAIRNVRRDGMDQLKKLEKDGGLSQDDHKVWAD
                     EIQALTDRHVELVNDLLQHKEKDIVTV"
     gene            3047..3796
                     /locus_tag="P5558_00240"
     CDS             3047..3796
                     /locus_tag="P5558_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRY22766.1"
                     /translation="MSAPALRSATPATGASAAPVHVAIIMDGNRRWARARGMPPVFGH
                     RRGADAVRRAVEACGNLGVRYLTLYAFSSENWQRPAGEVTELMNLLRFYLRKEIEALH
                     RNGVRLRVIGDRSRLDRDILELIDNAEALTREHQALELIVALNYGARDEIVRATRRLA
                     AAAVAGELAPETIDHGQFAAALDTSGVPDPDLLIRTSGEQRISNFLLWQLAYTELVFL
                     DVHWPDFSEEHLRLALEEFGRRERRYGACAG"
     gene            3804..4622
                     /locus_tag="P5558_00245"
     CDS             3804..4622
                     /locus_tag="P5558_00245"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRY22767.1"
                     /translation="MPADFAKRAIAGVVMALVAGLVIVLGGWYLVAVLMLVVVLAAGE
                     WAALTLQDSRKRYAVLGMVLLPLAILAGTHQAITAWRGTDMALVGGALGAVLLLVSGW
                     LIRRVGGWSERRWAVLGILYLGVPAVALLALRDLPDGLHLVLWLVAVVVCTDVFAYLV
                     GRTVGGPKLAPRISPGKTRSGLAGGMLAAAVAGALLAGWVGWGWFQAGLAGAVLALVA
                     QTGDLFESAMKRRAGVKDSGHLIPGHGGVLDRFDGFLFAAPVFALLVAIARTGA"
     gene            4627..5835
                     /gene="dxr"
                     /locus_tag="P5558_00250"
     CDS             4627..5835
                     /gene="dxr"
                     /locus_tag="P5558_00250"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRY22768.1"
                     /translation="MAASPVHRPPSAAAPRSITVLGATGSVGRSTLALVRADPAAYRV
                     TALVAGSRAAPLAALAREFDARLAVVADPAAYPELRAALAGSGIEAAAGTEAVLEAVI
                     RPVDWVMSAIVGAVGLAPTLEALRRGRMVAIANKECLVSAGGLLLATAKASGAVVLPV
                     DSEHNAMFQALVGHDRAAVERLILTASGGPFRTLDRAAMAKVTVEEALNHPNWSMGAK
                     ITIDSATMMNKGLELIEAHHLFGVPEARIEILIHPQSVIHSLVAYRDGSQLAQLGVPD
                     MRVPIAFCLGWPGRLAVEGVQLDLAKVGRLEFAAPDAERFPALRLARDALRSGGTVPA
                     MLNAANEVAVSAFLERRIGFLDIAAVVEQVLMQLPGDEPRTIDDIRECDGVTRRVARS
                     EVARRAAQHA"
     gene            5857..6978
                     /gene="rseP"
                     /locus_tag="P5558_00255"
     CDS             5857..6978
                     /gene="rseP"
                     /locus_tag="P5558_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRY22769.1"
                     /translation="MDLIDLVVNYAVPFLIILSVVVFVHEYGHYWVARRNGVRVEVFS
                     IGFGRELFGFNDSHGTRWKFSLIPLGGYVKMLGDADATSALPDPSTAWQEESFPAKRV
                     WQRMAIVAAGPAANFLFAIVVLSVLFVASGRPFTPAVVGEVQPESPAAAAGLEPGDRI
                     AAVDGQAIDSFESLQAAVRDLAEVPLEFTVDRDGANFTVTVVPALREIEDRFGNVHRV
                     GLVGISRTGIEFRRSNPAYAVFEATAETGRMIGATLKALGEMVVGTRGTDELGGPLRI
                     AQMSGTIAKDGLVPAIWFTAILSINLGLINLFPIPMLDGGHLVLYSIEAVIGRPLGER
                     TQEMAFRVGLAMVLTLMVFATWNDLVQLNVVKFVTGLIS"
     gene            7053..9326
                     /gene="bamA"
                     /locus_tag="P5558_00260"
     CDS             7053..9326
                     /gene="bamA"
                     /locus_tag="P5558_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRY22770.1"
                     /translation="MRRALGVIVSVCLLLAGSFGHESRAQVEDRILDIQVQGNERIET
                     LTVESYLTVRRNDPFDPDALDQSLRNLFATGLFDDVAIERVGSTLIVRVVENPIINRI
                     AFEGNRRLDDEVLEAEVQLRPRVVFTQARVQAAVTRILELYRRNGRYAASVDPKIIEL
                     DQNRVDLVFEIEEGPLTKVAGISFIGNASFSANTLRGVIETKEAAWYRFFSSTDTYDP
                     DRLAFDQELLRRFYLERGYLEFLVVSAIAELTPEGDRFFITFTLEEGPQYTLSSVELE
                     SSIPDLPLDALRELITPKSGDVYNATEVEKSIQSLTDRLGQLGYAFAEVDPITELNPD
                     ELLVDLIFQINEGPRVYVERIDIVGNVRTLDKVIRREFRLAEGDPYNLALLRRSEQRI
                     RNLGFFETVNVRTARGSAADKIVIQVEVAEQSTGELSFGAGFSTADGPLGDIRLAERN
                     LLGTGQSVSANFTISGRRQDIIVSYTHPYFLNRDLAAGFDLFLSETDYQSESSFDEES
                     VGGTLRAGYPLTENLRHGVRYTLREDEIRNVDNDASVFIQDEEGTSVTSLVGQTFTYD
                     VRDTRFLPSEGYILRLDQDLAGLGGDNRFLRNDVRGDYYYSIFPDVVLNVGAGVGYIH
                     GLGDDVGLSNRFFIGGSSLRGFAFAGIGPRDSDSNDALGGNLYYTGTAEVRFPLGLPD
                     ELRIFGRTFVDAGTLTEIDVNGPTLEDSGSLRVAVGFGFSWLSPLGPLAIDLAQAVRK
                     ENYDETELFRISFGTRF"
     gene            9340..9903
                     /locus_tag="P5558_00265"
     CDS             9340..9903
                     /locus_tag="P5558_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRY22771.1"
                     /translation="MTAGRRWRQLALTALLLLASGLPVMAEPLPPATAAVIDYQRVMR
                     EALAAQSIRAQVDARRLRYQQEIEADQQRLRNTDRELIRQRGILDADAYAEQRKAFEE
                     EVAQIQRQVQERLRQLDDVSAMALAEVREAVIEIVGDLAEVRSFNLVLPSAGVLLFSP
                     QIDITDEVLAALDERLPEVDVPERAPQ"
     gene            10032..10523
                     /gene="fabZ"
                     /locus_tag="P5558_00270"
     CDS             10032..10523
                     /gene="fabZ"
                     /locus_tag="P5558_00270"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRY22772.1"
                     /translation="MSEAARKPGRAMPDIEVGRLMQLIPHRFPMLLIDRLIEIEAPER
                     ATGIKCVTINEPFFQGHFPGDPIMPGVLIVEAMAQTAAALVIAGQEREGKGDLVYFMG
                     IDQARFRSPVRPGDQLRLEVELQKHRLGIHWFKGRAVVGGQVAAEAVFNAKLFGREQV
                     AGG"
     gene            complement(10540..11844)
                     /gene="gltA"
                     /locus_tag="P5558_00275"
     CDS             complement(10540..11844)
                     /gene="gltA"
                     /locus_tag="P5558_00275"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRY22773.1"
                     /translation="MTESTTEKSVKIGRGNQQSEFPVLEGTIGPDVIDIRKLYSATGH
                     FTYDPGFTSTASCRSKITYIDGEAGLLLHRGYRIEELAERSNFLETCYLILFGELPKA
                     DEYKKFVDGVTNHTMIHDQINYLFRGFRRDAHPMAVMVAVVGALSAFYYEDLDTQDPK
                     HRLIASYRLIAKIPTIAAMAYKYSVGQPFLYPRNDLNYAENFLYMMFGVPCEPWKEKP
                     VLSKALDTIFILHADHEQNASTSTVRLVGSTGASPYAAISAGIASLWGPAHGGANEAV
                     VNMLEEIGTVDRIPEFLARAKDKDDPFRLMGFGHRVYKNYDPRAGVLKASADEVLDEL
                     GKGGEPKLAIARELERIALEDAYFQERKLFPNVDFYSGIILEAMGIPTKMFTAIFALA
                     RTVGWIAQWNEMIEDPEQKIGRPRQLYTGPAERSYVPLDRRG"
     gene            complement(12008..13435)
                     /gene="gltX"
                     /locus_tag="P5558_00280"
     CDS             complement(12008..13435)
                     /gene="gltX"
                     /locus_tag="P5558_00280"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRY22774.1"
                     /translation="MSVIVRFAPSPTGYLHIGGARTALFNWLFARHHGGRYLLRIEDT
                     DRQRSTEPAIAAILDGLGWLGLESDEPPVYQFSRIEQHKAAVQRLLEQGLAYRCWCTP
                     EELEAMRERARAEKRPIRYDGTWRERDPREAPAGVDPVVRFKAPLDGETVVEDRVQGT
                     VRFANTQLDDMILLRADGTPTYMLSVVVDDIEMGITHIIRGDDHLNNAARHANLFRAL
                     GHEPPVFAHIPLIHGPDGAKLSKRHGALGVDAYRELGYLPEAMRNYLLRLGWAHGDEE
                     IISTQQAIAWFDLDGIGRSPARFDFAKLDSVNAHYLKERADNELVTLVAPRLEAAGHR
                     LGEPERRRLEQAMPGLKQRARTLVDLASSAAFYIQARPLPLDAKAAKLLDPAARAELA
                     RLAPVLAAVEPFAEAGLEAAARAHAERQGLGFGKLAQPLRAALTGRTVSPGLFEVMAI
                     LGREEVLLRLEDAATGRNPAMQQSD"
     gene            13510..15639
                     /locus_tag="P5558_00285"
     CDS             13510..15639
                     /locus_tag="P5558_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY22775.1"
                     /translation="MQQPALVPSARSWAPRPAGGSRLLLALVAVVLESLHAERPRWPL
                     WAPIAFAVGILVYFALPVEPSPRLLALVPLGVIGAIAGARRAAGLAVLFWLGLALALA
                     GFAVAQLRTLQVAAPVLPRSGVYHVEGVIRAAEPRNDGQRLLLGDLVIERLEEAATPA
                     LVRFTVRRTEVPFLPGQRIKARARLQPPSGAAVPGGFDFAMRAWFERLGGVGFALGAP
                     ELLAAGPGTGTRPIATLRHAVAARAERIIPGADGAVAAALLTGLRASIPDRVWHDMQA
                     SGLAHLLAISGLHMTLIAGTVFVVARALFALWPALALRVVPKKPAAVVALLAALGYLL
                     LAGATVPTQRAFMMAAVALLAVLLDRNPVSMRLLAMAAFAVLVLAPESLLGPSFQMSF
                     AAVTGLVAFYELRLRRLAAAPPDSDRQRPGRLLLVYFVGILVTTLIASLATTPFAAYH
                     FQRVATYGVLANLVAVPLTAFWIMPAGLLALLLMPFGLDAPILHVMGAGIGIVLDVAA
                     FVAALPGAGIDVSPWPPSSLLLLALGGLWLCLWRQYWRLWGGAACTLGIVLALTTRPP
                     DLLVDRRGDLVVYRAAGGEVHLLELKRDRWLAEQLVRAAGSGEASAFETRSDGQLACD
                     PLGCVVGPEPARRVAIALTAEAAAEDCDRARFVLLLAGPESCANGTPSLGARTLWRSG
                     GVALTIADGRVRVETVADWAGDRPWMR"
     gene            15717..16187
                     /locus_tag="P5558_00290"
     CDS             15717..16187
                     /locus_tag="P5558_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22776.1"
                     /translation="MLAHPTHPFARIDTLRHHALARPLMAALLLVVGGLLLYSVAALV
                     DRRDAAATFVPGSGIGLLGSGGVQALTLPLATERQPAREPTPRPSATDSIVAPVLAAE
                     VADDLKVMLAGTLEQVAPAAGMPRPPLDAAAPPLRVEIMGLADTAWTSVATTNR"
     gene            16157..18385
                     /locus_tag="P5558_00295"
     CDS             16157..18385
                     /locus_tag="P5558_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY22777.1"
                     /translation="MDVGRHDQSVAQADPVMHPSHAAHPAVGPPPEAGRELSLPELLA
                     VLRRYRLLLAGALGVGLGMAVLLLAWLPPVYLATAQVMIEPMRQGPEDSALPAAGIAD
                     DGTAIDSQVKLLASRSMARQVIEAMGLETEPELQRSLDVAERLGDLAGVARAAAREDD
                     AIGLIDRFLERLEVARDGKTHVIRITFRSSDPGVAAQVANGVAERYIAGQLEAKARAT
                     RDAADWLRTALDSSREVLEVAESELASFRENVQDDYADAFALHGVDVVNLRRDLVAVA
                     GERAARQSELGRLRRVLAENNSDLAFQELGGSEVLEDLYNLKNQTTRREAELATQYGD
                     RHPLILDVRAERRELERRIRAEQLALVGSVEAEIAAARAREAALQRELEGLKSQNSAL
                     AQAEARIADLERAADRARRLHEAYLARYQAVADAGDTQRPDARLISEATVPPRPSQPR
                     PALIFSLFGAASLGTGLLLVFFLEQLDHGFRSVRALEQGLGLRCLGAVPLVDRRRRRG
                     LALADLPLERPRSRFAESLRALIAGLVGPAVEGAGRVVLVTSSVPGEGKSTTTTALAR
                     LAAAEGLKVLLIDADLRRPILATLLGLEGETGLVEILRGEREAEAVLRVDRRSGLSFI
                     PGSARVSQPTRLLGPAAMGILLDEARRHFDLVLVDSPPLLAVGDARVMAPLCDAVLLL
                     VRWNATQQALATHALSQLGQAPGRPVLAVLTGVDPDAQLGLGSGESRVVRRQLLRYYA
                     EG"
     gene            18415..19743
                     /gene="gltX"
                     /locus_tag="P5558_00300"
     CDS             18415..19743
                     /gene="gltX"
                     /locus_tag="P5558_00300"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRY22778.1"
                     /translation="MTFNLRFAPSPTGRLHLGNARTALVNWLQARQAGGRFVLRLDDT
                     DRQRSTEAFARSITDDLAWLGIDWDVCIRQSERAALYATAFAGLREQGLVYPAYETAE
                     ELADKRAAQRRLGQPPRYDRAALALDAADRAQLEGEGRAPHWRFRLSGAAIAWTDLVQ
                     GSVSIGTAALSDPVLVRADGVATYTLASVVDDLECGITQIVRGDDHRTNTAVQIELMR
                     ALGGMPPVFAHLPLILGAGGAPLSKRSGDWSLGDWRARGIEPHAIRLYLASLGTDRVA
                     PPETSREALIREFDIGRFGRAGAVFDADQLARMSRAVFRALPLEAVRDRPGCAGIAAA
                     EWAALRPNVADAGELARWLAVLQEPIKPVREDAELLGAAADLLPLALDDASAAAWLGA
                     VQESTGRRGKALYHPLRLALTGRTDGPKLADLLPLLGRERVLRRLRGLTA"
     gene            19776..21131
                     /gene="cysS"
                     /locus_tag="P5558_00305"
     CDS             19776..21131
                     /gene="cysS"
                     /locus_tag="P5558_00305"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRY22779.1"
                     /translation="MTLVLHNTLTRAEEPFSPLVAEHVRLYVCGPTVYQRIHVGNARP
                     LIVFDVLFRLLSHLYPRVTYVRNITDVDDKIIEQARANGEPIEALTARTTAAFLEDAA
                     ALGCLEPTIQPKATEHIPEMVALIGRLIERGHAYAAAGHVLFSTPSMPAYGRLSGRNR
                     DDQIAGARVEVAPYKRDPADFVLWKPAAGDEPGWPSPWGRGRPGWHIECSAMAEKHLG
                     LPFDIHGGGQDLIFPHHENEMAQSCCAHGLETMARYWLHNGFVDMRGQKMSKSLGNVL
                     PMDDALAAAPGEAIRLWMLGAHYRAPIDCSESGFAEARTRLDRFYGALERAGETTADV
                     PDPELIAALEDDLNTPKALAVLHELLHELNRASHSAAPAIAGRLKASAGLLGLLEAPP
                     SKWLRGTLDDSRIAGLVAERQAARQARDFARADAIRDELAAEGILLEDGPGGTTWRSG
                     Q"
     gene            21128..22720
                     /gene="cimA"
                     /locus_tag="P5558_00310"
     CDS             21128..22720
                     /gene="cimA"
                     /locus_tag="P5558_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRY22780.1"
                     /translation="MTERLRLYDTTLRDGAQTQGITFSAADKRRIATDLDALGIDYVE
                     GGWPGANPTDDAFFATPPALGRAKLCAFGMTRRAGRSAANDPGLQPLFQAKVPVITLV
                     GKSSRRHAEGALGVDGDENLRMISESLVESRRHSEEVMLDAEHFFDGFKADEAYALAC
                     IEAALEGGADWIVLCDTNGGTLPQEVTRIVRRVAALVPREKLGIHTHDDTGNAVANTL
                     MAVLEGATQVQGTLNGLGERCGNANLVSLIPTLKLKMGYDVGIDDQALTRLTALSRGF
                     DERLNRTPSRGAAYVGANAFAHKGGLHASAVSKDPRYYEHVDPALVGNQRDILVSDQA
                     GRSNLMQRFAEMGLEIEPAPDEVAALLATIKEREARGFSYDGASASFEILVRRTLGRL
                     ADHFELLSFRVIDERRFNAVGELVTQSEATIKVQVDGRDFYTVADGNGPVNALDHALR
                     QALAPTYPTLAGMELVDYKVRILSPEAGTAAVTRVIIESADDAGRVWQTIGVSGNIID
                     ASYNALHDAITYKLLLDGLAER"
     gene            22722..23537
                     /locus_tag="P5558_00315"
     CDS             22722..23537
                     /locus_tag="P5558_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY22781.1"
                     /translation="MTSGPDNAPPVAFKRTKAARGGIDALEAAPVVILVRPQLAENVG
                     TTARAMLNFGLGELRLVAPDFGWPNAKAVAAASGAAEILNRMQIFPTVAEAAADLHHL
                     YATTARDRGLVKEQHDPASAAAAARAQVAAGRRVGLLFGPERTGLDNDELALADGLVR
                     IDVNPLFPSLNLAQAVLLCAYEWRRQGAEPSAMTAPEPAPKAALDGLLRHLFVELEAA
                     GYFRSPDRRDSLGRAIAAMVERMRLTAPEVQLMRGIVKELRRGPLKRPGDDAG"
     gene            complement(23548..26838)
                     /locus_tag="P5558_00320"
     CDS             complement(23548..26838)
                     /locus_tag="P5558_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY22782.1"
                     /translation="MSWRLLLVLWALLDTGIGLLPDVARAAEAMVRIGLHNNYGRLVI
                     EWPAPVEIQTAREGAGLKVQASHPFPARLSELSGRLHRYLAALKVDADGAGLAVEATP
                     GVDLTFELLDERLLVLDFRAAPTELRLRVGQHPGFVRLVIEPVATAASLLQRSPAGLE
                     LDLPGPLSAWALGRLREVPGITGVTLDGNRVALGLAATATARDLLVEPDKLVIDVTPP
                     PDAIGAPADTVVSPASPNFPLPHARPTPTAVVADTGMPAEKAPIVHAATDLGDAPVAP
                     AALPVEGPVVITGTRRGVASTEIAFRWAAPVPAAIFVRGRSLWAVFGAQANAIEIDTE
                     AFATAVGQHITGLRRERHGSATVLRLALTDERSALVHRDDASWVVTLRPPREPAGATG
                     APALAPEADGLLLPGITSVASLVDPVIGDHLGVGLALAPTGPRLVESRFVGLRLLPSA
                     QGAVWRALAEPVEPDDLTTAGLLLAPAHGVLPTAAPPAVVAAPSTEPTFAHLPMEESA
                     RLPVDSHAADDNAGPHPVPHHEPPGEHRAEAHSPPSPLGLARFAEDDFRTRRAALLTA
                     LTTADRPSREAARLDLARLLVVNGLGAEVLELLDAATVETAEPGSTSPPAQAALTGAA
                     LLLMDRPAEAAEQLADRKLAADDETALWRGAAAAATGAWDQGLADWQRGFAHLATYPL
                     DLQATLALPGVRLLLETGRADAAFELIDRLTVQDLRPDRLRSLRRLEATALERDGATD
                     EALAAWQALAGNGPLEERAVARAAATALALAAGQISIDEAIATLEADRRHWRGQQEEF
                     TLWRRLAGLQQEAGRIDEALDTLRTAMHRSPPAAEAQAITREMAAFFAEALDELAAGQ
                     RSPTSALLLYRRFAELMPVGPEGDEAAATLSAALLATGLATAGTGILDDRLGLHAERD
                     ARRARLGLMLARLHLASGAPDRALATLVDSTPLGAVDAELTADRQQLMAEALAANSTA
                     TVLTDGPAAGLLRARAAFDAGDWPAVRVAASPLEVALPESGPIDTEAGEIVLMLATAA
                     RQLGDAEEVRRLARRHAERLSSERDAALLGLLAGLPDFAGDTDRVLADATRHLHEARD
                     VLAVLGRH"
     gene            complement(26835..27467)
                     /locus_tag="P5558_00325"
     CDS             complement(26835..27467)
                     /locus_tag="P5558_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22783.1"
                     /translation="MRAERHLAGQRAWSIMLADVVALLLTFFVLGLSMRELDGGPATG
                     LMPIDPDRPVIALVMQGTVAERAAPEISAEADRSFAYLAALLREQGDLATSANISYTE
                     FQLNIDMADPASSASSAERSALTELMATYAYLARRFALQASFRLPTRVDETLPARLDR
                     VLAFQHRLTQELGLVDAEVVVAAAGEPGGDRLRLALRTQPPPGDTLTNPP"
     gene            complement(27457..28110)
                     /locus_tag="P5558_00330"
     CDS             complement(27457..28110)
                     /locus_tag="P5558_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22784.1"
                     /translation="MHAARYQATRADAPSGTRLDPAPAESPTDGFLKLMLILLAFFVV
                     LYSRSEISATKAGPVLEALADRFAGPSGSAADNEPLDGSVRAEQQLRRRLAASLPVQA
                     SARELPGMLIAFELGEGDLFASPGNAVRRERLVLLRRLAIALAGRDAAWDTPLVVTTA
                     WPEAVGDPARDRLAALAEVLAANGLAGAALSLGFAALAPGTWRFAVPRVAVRRADAG"
     gene            complement(28287..31043)
                     /gene="fdhF"
                     /locus_tag="P5558_00335"
     CDS             complement(28287..31043)
                     /gene="fdhF"
                     /locus_tag="P5558_00335"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRY22785.1"
                     /translation="MTDPIRFTLDDQEVEAAPDETIWQVAERLGIAIPHLCYAPEPGY
                     RPDGNCRACMVEIEGERVLAASCLRTPSPGMKVHVGNERATRARHMVMELLLADQPPR
                     ERARDGESRLWQWMERSGLETSRFPAEARPAPDPSHPAMRVNLDACIQCTLCVRACRE
                     VQVNDVIGMAGRGHHEKIVFDMDDPMGLSTCVACGECVQACPTGALMPASIMDEDEIG
                     DRSVESVCPFCGVGCRVTFHVRDDRIRYVSGANGPANENRLCVKGRFGFDYVHSPERL
                     TVPLIRKADAPKRADELVDAANPWSHFREATWEEALETAAAGLRRIRDAHGGDALAGF
                     GSAKGSNEEAYLFQKLVRTGFGTNNVDHCTRLCHASSVAALMEGIGSGAVTAPFNEAL
                     LADVIVVIGANPTENHPVAATYFKQAVKQGAKLVVMDPRGQALRRHATHMLQFEPGRD
                     VALLNAMLNVIVTEELFDRQYLQAHTEGFDHLRQHLAAYPPEAMAPICGIDASTIRTV
                     ARLYARAERAILFWGMGVSQHTHGTDNARCLISLALLCGHTGRPGTGLHPLRGQNNVQ
                     GASDAGLIPMFLPDYHRVDDPAYREQLERLWGATLPERPGLTVVEIMDAAVDGAIRGM
                     YVLGENPAMSDPDTSHVRAGLAKLEHLVVQDIFLTETASFADVVLPATAWPEKTGTVT
                     NTNRQVQLGRPALPVLGETRPDWWIVQELARRLGLDWRYDGPEEVFAEMTLAMPSLAN
                     ITWKRLEREGTVGYPCPAPDHPGQGVVFADRFPTASGRATLVPASGLDPAEAPDARYP
                     MVLTTGRTLEHWHTGAMTRRATMLDALEPEAFVAMSGEDLGELGIAPGERVRISTRRG
                     TIELMARRDPAVARGMLFIPFCFVEAPANVLTNPELDPFGKIPEFKFAAARIERIGES
                     GGTA"
     gene            complement(31138..32049)
                     /locus_tag="P5558_00340"
     CDS             complement(31138..32049)
                     /locus_tag="P5558_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HRY22786.1"
                     /translation="MARTTQPAGAAGGAGTTLVDGVGSFVDRFDAFLIDQWGVLHNGA
                     APYAGAIELLAELHRRGKRLVLLTNSGRRRSYNAERLLELGFDTSVLTGIVTSGEATW
                     LALQNRTLDAFQALGRRCLLFTREGDMGPVEGLDLEVVEDPGQAEFVYLTWLDTLTHD
                     MAEFDRIIAVAAARGLPMVCSNPDRVAPTEGGLTMAPGALAERYHNIGGRVIYVGKPH
                     APIYAACRDMLPGVAAEAICGIGDSFEHDIKGAKDAGLAAAFIMRGIHAEAFPEGADP
                     AADRAALENLARRHGAMPDYALPGFCL"
     gene            32091..32837
                     /locus_tag="P5558_00345"
     CDS             32091..32837
                     /locus_tag="P5558_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY22787.1"
                     /translation="MTPLDPGLEGKRVLITGASRGIGAAAARLLAACGARLVLHHRRD
                     AAEAATIPHVALVQGDFTERAAPARTVEAAVAALGGLDILINNAGHMLGRITLDAMDD
                     AAIDRVFDLNARSVVAASRAALPALRASSGQIINVTSISARTGGSAGSALYSSAKAFV
                     STFTRALATELAADGIRVNAVSPGTIATEFHEVYSSSDKLEATRQRIPLQRLGQGDDC
                     AGAFLFLASERLSGYVSGQILEVNGGQLMP"
     gene            complement(32858..33292)
                     /locus_tag="P5558_00350"
     CDS             complement(32858..33292)
                     /locus_tag="P5558_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22788.1"
                     /translation="MLASTSPAGPPVPPEARATATGPGLWTPPIDGHGLYKSLILRLP
                     LEVRRSLDHEQLLALRQATEELAWGGHTVDIRVSLWTPLGRRYLAIVGGRERRDQLRL
                     AHERSRHPLVRLGNVVSLLLFACMVLGLGAFSGLAVLALTYL"
     gene            33654..35090
                     /locus_tag="P5558_00355"
     CDS             33654..35090
                     /locus_tag="P5558_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY22789.1"
                     /translation="MRELLAEDRRGGVLLFFGLILVPLVGFAGLAIDSLVAFSVEARV
                     QRALDAAGLAAGRAAREEDVVTEARAFFDANLGATGDEIAISGFDAVYSPEDESINLS
                     VRATVPTYFMRVLGKTETTIDLQTVVNLQIRQMELVLVMDNTGSMRSGGKINAMKDAA
                     AELVRIVFGGRESHPNLWVGLVPYVASVNIGAHRTGWLAADDPVLIGAGASPSAGKKK
                     GKNEPAGPVDPFAPSSWKGCVMARPAPFDETDDPPTVEPFSSYLYPSGSDNNWPPLDE
                     SNAAENNGRGPNLGCGPAITSLRQSRAEVLASIEEMLPWHRGGTTGNLGLVWGWRVIS
                     PRWQGLWGGATPAELPLAYNDAESDKVVVLLTDGDNQFYDNPPSGPSGSDFTAYGRLS
                     DFGFASLAAGRDELDRRMARTCAAMKAEGIMLYTITFGPTPNSGTQTLFRTCATSPGH
                     YFHSPNNSDLSSVFRAIGRQLSSLRVAR"
     gene            35087..35659
                     /locus_tag="P5558_00360"
     CDS             35087..35659
                     /locus_tag="P5558_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRY22790.1"
                     /translation="MSDGRRQRFGAARAFGPDRTGNTMVEFALIGPVFLVMVLGIFEI
                     CLILFTSGSLQSAVASASRYGITGQAGDTDRLTAITEILRDRTFGFIDIEEADISTLV
                     YPSFTEIGRPEPFTDQDGDATFDPGEPYEDINGSGGWDADMGSAGLGGPGDIVLYNVD
                     YRSRLMNAFVAPLFGDIRYTATIAVRNEPY"
     gene            35675..36412
                     /locus_tag="P5558_00365"
     CDS             35675..36412
                     /locus_tag="P5558_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRY22791.1"
                     /translation="MLPGVAHAARPRIYAFARAAHIGAWRMWVAGLRGRLSGDLCRGH
                     LAADRRGVIALEFALILPLLILFCFGTVEVGRFVLLQMKLDQLAASVADLGTRDRTLS
                     AATIDDIFAAGAHIVRPFDLAADGRITLSAVGLTAGDQPRVLWQRRSGGTLDLPSETG
                     TVGSPATLPDEITLAVGQTVVVAEVSFSYRGMFGLLPERITTKRAYFRPRLGSLQELS
                     SIGGSDHPLIADAGWCESLQASVVASG"
     gene            36561..37613
                     /locus_tag="P5558_00370"
     CDS             36561..37613
                     /locus_tag="P5558_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRY22792.1"
                     /translation="MSPDGVLFRRLRRVVPHGSRGAAFRCSMTPISALVRPALSSLEK
                     KLTRAVILYPTAPFMAADRRVALERWLRGREQCQKLGKADIVIVSFGKSGRTWLRVML
                     SRFYQLRHALSERFLIGFDNYHFMNRAVPRIFFTHDNYVKDYSGNVDNKADYYGKRVV
                     LLVRHPADVAVSQYHQWRFRMRPRKKVINDYPDEAGEVSVFDFVRHQPAGLEKTIDFL
                     NGWARERERLGACHILRYEDMRADPAGSLGQLLDFVGTPGTPAEILGAVDYASFENMK
                     KLEGQSRFWLSGGRMMAKDKSNPESFKVRRGKVGGFRDYFTDAEVDSILDYVDERLDP
                     VFGYRRTVMAPSGSEV"
     gene            37648..38550
                     /locus_tag="P5558_00375"
     CDS             37648..38550
                     /locus_tag="P5558_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22793.1"
                     /translation="MSEPRPCVFIHTNPKQMLGARVSEYSFRRNSARPDAFDVRIIST
                     EDFPWLKSHEGRTYRRDGLDREWLYDDLQSFTPLRFTPPELMNYSGRAVIVDPDVFAV
                     GDILDLLKRDMAGKPLFARPRGGSKEFASSVMLLDCARLTHWRAEEQFDELFQGKRDY
                     MDWICLKLEPRETIGLLEPEWNDFDRLTPSTKLLHNTKRWTQPWKTGLPVDFTPSSKT
                     RRFAPLGWFRRAREAVLGRYGGLGTYKRHPDPGQEAFFFGLLKECLDKGHIDEAMVRA
                     EMAANHVRHDALEMLERSTLQQAA"
     gene            38570..39571
                     /locus_tag="P5558_00380"
     CDS             38570..39571
                     /locus_tag="P5558_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY22794.1"
                     /translation="MSWAERRRYWRKRWRRLAVVRSLRLGLRDAFLLSFWAICARLSP
                     TRASRLGAACSRVLGRLSTKNDKIRQNLRIVFPQAGRGELDRIEQAIWSNMGAVIGEY
                     PHLGVLGRSAGDGVGLESAIPPHLEPNFSGERLTVFVAGHLANWEVLAVAPAHWGVDL
                     AVIYTPIGEGAADRRLREYRERMSARLLPRDGSARELLRHLRSGRPVGIVADHRADEG
                     ASLPFFGATKRTTLSPARLALRTGAELVAVRVERLGPAHFRLSAEGPIRPPAGVGDEE
                     TKAVAMMTAFNACLERWIRERPGEWLCSKRAFPKEVVDSLPSRPLGGSLKPDISQHA"
     gene            39564..40511
                     /locus_tag="P5558_00385"
     CDS             39564..40511
                     /locus_tag="P5558_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRY22795.1"
                     /translation="MLSDNVKKALVLAASQPLPPAWRLRLRRPGLARLELEKATRTDL
                     LIIGHPKSGNTWLRVLLSRLYQTRHNLPADLVVTSDELHRRNAAIPRISATNGWYSYE
                     GVVGDALAVDAPPSPIRDKPIVFLPRHPLDIATSWYLQVTKRQSNAKFELINADLDRP
                     IDRTRIDRWAFVRHSGFGLANIIAFLNTWQARVARLPHAITVPYEALRADPASQLARI
                     GELMGDPFSADEIAEAVRFGSFDNLKSLESSGFFKQKGLRPGIRGDAESSKVRRARVG
                     GYREDFTPEQVAELEALVAASLDPALGYAPRPASAGAEP"
     gene            40508..41665
                     /locus_tag="P5558_00390"
     CDS             40508..41665
                     /locus_tag="P5558_00390"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HRY22796.1"
                     /translation="MSDAPVTWLLLGDKRGDNRMVEVIAEHLPWPSITKRLVTRPEWV
                     VPKPRVRSTLDHLDLAASDPLEPPWPDLVLTIGRRPSMAARWLAEQAEGRTKLVLVGK
                     PSGSIEPYDLVIVSAEAFLPPSTRVMTIDLPLMRIDPTLVAAEAELWRPRLAALPRPW
                     IAFLLGGPTGPFRYDDELVERVLDGARRTASGEGGTAFIVTSRRTPKPVVERLRQELL
                     PAARLYAWGDEAAGNPYLALLGLADALLVTADSISMLTEAARLGRPLSVIPVSTPGLG
                     RLDQWRRELVQRLFGGRAARYAPLLGRLGISAQTRDFAAFERLLVQRGLASSYTIRPP
                     APTGRAGDDVGRVVARLCQLMGHALPKPQSAANSVDATPVAADRVVGVQSR"
     gene            41672..42421
                     /locus_tag="P5558_00395"
     CDS             41672..42421
                     /locus_tag="P5558_00395"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRY22797.1"
                     /translation="MSRGMWTCPATVQPNGVVPGLNRGESGMAQAIARATNSLSPVPP
                     PFDYARLAATERRDDPFPHVLVENFVTVPDRAALISDFPAITGPGNVEPGDVPQGPAF
                     DRLILELESDRFRAAIADKFGIDLSNAVPTIGIRAFAEPTDGRIHCDHRSKLITLLLY
                     FNEEWTDEGGRLRICRDQSDIEDFATEVVPLSGTLIAFRNGPTAWHGHKQHVGQRRML
                     QLSFRDMSGIVGIERRLSRLTKPIRRMLNLS"
     gene            complement(42408..43364)
                     /locus_tag="P5558_00400"
     CDS             complement(42408..43364)
                     /locus_tag="P5558_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRY22798.1"
                     /translation="MRHELVVGLGYGLRQGLFFLPRARRIGIERWLRGWEETRLLARA
                     DVAFVSYAKSGRTWLRTMLSRAYQLAYDIPEGTALEFDNLKRLDPRIPSVLFTHGNYL
                     RDYLDQWTDRAAFRRKRVLLLVRDPRDTAVSQYFQWKHRMHPAKKALNAYPPHGSGHS
                     VAEFVLQEPAGLAGVLDFLELWERELPQLDSVQVVRYEALRGQTAAVLSSILDFLGTP
                     APMGAVEGAVAFASVENMRRLEAAGADAGYKGQRLKPGDVANPESFKVRRGKVGGWRD
                     YLSAAECDAVEAMVQARPGQLFGYKTNTIGDSSDTGAAISSG"
     gene            complement(43481..44782)
                     /locus_tag="P5558_00405"
     CDS             complement(43481..44782)
                     /locus_tag="P5558_00405"
                     /inference="COORDINATES: protein motif:HMM:NF021524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HRY22799.1"
                     /translation="MNFSRIRFHLNRLGERLWLMPSIYCIGAVSVTFLARFADGTGLG
                     DWVPKIDPETLQILLQVIATTMLAVATFAVSSMVGAYNSASQAATPRAFRLVVADDRS
                     KRALSTFIGAFIFGVVGLVAVRVGIYGQAGNFVLFLMTLGVFAGVVLTFVYWLDTIAR
                     LGRMGETIARVESAALRAFANRRRRPRLGGRPVEGGQDPGRPVAGDSIGYVQYIDIDR
                     LQRAAEAADLRLTLAALPGKFVAPGQPLVHVARDWSMRGEVPDDEIDEDELRAAFLVG
                     AERSFDQDPRFGLVVLSEIAGRALSPGINDPGTAIAIIGAQVRILATHAAEYAEPAAD
                     EVACDRVHVPELAWSDLLDDAFNAIARDGAGLVEVQIRLQKALSALGRPSCAALTRAV
                     PHFATLALARAELALRLDDDQSRVREAAGRAAAGDTSKDSD"
     gene            complement(44779..45528)
                     /gene="cobA"
                     /locus_tag="P5558_00410"
     CDS             complement(44779..45528)
                     /gene="cobA"
                     /locus_tag="P5558_00410"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRY22800.1"
                     /translation="MSTATTVSGRVYLVGAGPGDPELLTLKAARLLGRADVVLHDRLV
                     GAGVLEHCRPGAELIFVGKRRAEHHMRQAEIHALMIDRARAGLLVVRLKGGDPLIFGR
                     GGEEIESLALAGIPVEVVPGVTAAAGCAAQSLIPLTHRDLAQTLILVSGHSQDGEPAL
                     DWQALCRPRQTLVFYMGHAVLDRLTARLIGHGLASDLAACLILNGTLPTARRITGTLA
                     TLPELVRGAEPLDGPALLVVGDVVDRARGPA"
     gene            complement(45567..46313)
                     /gene="cobS"
                     /locus_tag="P5558_00415"
     CDS             complement(45567..46313)
                     /gene="cobS"
                     /locus_tag="P5558_00415"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRY22801.1"
                     /translation="MMLRDLAIALVFLTRLPLRPNGPVGMGDLASVVHWFPVVGVLVG
                     VAGGAAFTLAHFLHLPSWPAAVLALGAMLLLTGALHEDGLADTADALGALPDQARALA
                     VMRDSRIGSFGTLALILVVGGRLGALAGFWEPLRAIAALVAAAAFSRALMPLVMWAQP
                     SARSQGLAATAGRPPTDRVLIGLAIGATLTILILPTGQGLGAMLAAALVAAFTAKALG
                     RAFGGCTGDTLGAVQQLSELTFLFALVAWR"
     gene            46403..47455
                     /gene="cobT"
                     /locus_tag="P5558_00420"
     CDS             46403..47455
                     /gene="cobT"
                     /locus_tag="P5558_00420"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRY22802.1"
                     /translation="MSSLTPLANFADLRYLLRQLPAIDGNAVAAARAREAVLLKPAGA
                     LGRLEQIASWLAGWQGRHPPRIEHTAIFVFAGNHGVADQGVSAYPATVTAAMVESFEA
                     GGAAINQLARIAGAELHVVPLRLDRPTHDFTLQPAMDEAACVAALNTGLTAPDDALDV
                     LAVGEMGIGNTTVAAALATALFAEAGEHPDPAQWVGAGTGVDATGMAQKSAVISRALA
                     RHEAVLGDPLEVLRHFGGFELAAILGAIVAGRLRRTPVVLDGFVATAAAAVLQRLRPD
                     ALDHCLAGHVSAERGHRRLLAELGLRPLLDLDLRLGEGTGAALALGLLRAAAACHSGM
                     ATFAEAGVAASPNKGA"
     gene            47716..>48560
                     /locus_tag="P5558_00425"
     CDS             47716..>48560
                     /locus_tag="P5558_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22803.1"
                     /translation="MRAKIDNVTPAERVGRASQELAALRQEEPTGEAVAVPAQLGALQ
                     GYFIEIDNRQIFGLLPQDVDPLQRETLEQAAIAAAGRLNAALRVRAEQRSLPMILRSF
                     GLASLATAVLVLMILGIVWLRRLTLRQLTALSRGNAITVAGIDLRPYSRATGTAVVKA
                     LGVAAQLVCVYLWLAFVLLQFSYTRPWGEGLGIWLISLLGEFSHAILSAVPGLFTVAI
                     IFIMTRFVADLVKGFFKRVEAGWLRVHWLEAETARATRRLVIVLLWLFALTVAYPYIP
                     GSQTDA"
BASE COUNT         7306 a        16446 c        17121 g         7687 t
ORIGIN      
        1 cgtcgtcgcg ggcggcggcc gggtgctctt cgtcggcacc aagcggcagg cgcaggagcc
       61 ggtcgcccgc gcggcgcagc gctgcggcca gtactacgtc aaccaccgct ggctcggcgg
      121 catgctgacc aactggcaga ccatctcgaa ctcgatccgc cgactgcgcg agctggagag
      181 ccggctccgc gagcagcaga gcgggctgac caagaaggag ctcttgcagc tcagccgcga
      241 gcgcaacaag ctcgagcagg cgctgggcgg catcaaggag atgggcggcc tgcccgacat
      301 cctcgtcgtc atcgacacca acaaggaaga catcgcggtc accgaggcga agaagctcgg
      361 cctgccggtg gtggcgttcc tcgacagcaa ctcgagcccc gagcacatcc gctacccggt
      421 gccgggcaac gacgatgcca gccgggcaat caatctctac tgcgatctcc tcgtccaggc
      481 cgtgctcgac gggttgcagg ccgagatgac ggcgagcggc atggatctcg gcgagatcga
      541 gccgatcgag cccgagctgg cggagccgga gctggccgag caggagctgg tccactcggc
      601 gccggtgcag ccgctggccg acgcgcccga ggagaccggg cccgagatgg cgccggtcga
      661 ggacacgaag gcctccggcg ctgccgccag ctgagcgggc gggccgagac gcagagacaa
      721 cacggatacg gaagcgatta tggctgagat aacggctgca ctggtcaagg agctgcgtga
      781 gcggaccggg gctggcatga tggactgcaa gaaggtgctg atcgagaccg cgggcgatat
      841 cgagggatcg atcgacctcc tgcgcaagaa gggcttggcc gctgcagcga agaaggccgg
      901 ccggacagcg tccgaggggc tgatcggcat ggcgctcgag gggacggaag gggccctggt
      961 cgaggtcaac tccgagaccg atttcgtcgc ccgcaacgac gccttccagg cgctggtcac
     1021 ggcgatcacc ggcgaggcca tcgccgccgg tggcgatgtc gagacgctgc gtgccgcgac
     1081 cctgccgacg accggcaaga gtgtcgccga ttcgctcacc gacgcgatcg gcgtgatcgg
     1141 cgagaacatg aacctgcgcc gggcggtgaa ggtcacggtg ccgacgggcg tcgtcggcgg
     1201 ctacctgcac cagcaggttg cccccgggct cggtcggatc ggcgtgctgg tcgggctcga
     1261 atcgaccggc gacaaggcgg tgctggccga gttcggcaag cagctcgcca tgcacgtcgc
     1321 cgcggccaac ccgcaatcgg tcgccgtcgc cgacctcgat cctgccgtga tcgagcgtga
     1381 gcgggcgatc ttcgccgatc aggcgcggga gtcgggcaag ccggaatcga tcatcgagaa
     1441 gatggtggat gggcgcctgc gcaagttcta cgaggagagc gtgctgctcg agcagacgtg
     1501 ggtcatcgac accgacaaga aggtcaagga ggccgtggcc gatgccgcca aggcggccgg
     1561 ggcgccggtg aaggtcaccc gcttcgagcg cctggcgctc ggtgagggga tcgtcaagga
     1621 ggagagcgat ctcgccgccg aggtcgccaa gctgaccacg gtctgacggg acatcctggg
     1681 atcgggaagg cgcgcatgag aggagtcgtc gggcgatgag cgaggccgag acagtgacgc
     1741 cacgctatcg ccgcgtgctc ctgaagatgt cgggcgaggc gctgctcggc gatcagagct
     1801 tcggccacga cagcaaggtc ctccgtcagc tcgcttcgga ggtcaaggcg gcgcacgcgc
     1861 tcggcgtcga gatctgcatg gtgatcggcg gcggcaacat cgtgcgcggg gcggccctcg
     1921 ccggagccgg tgtcgagcgg gcgagcgccg actacatggg catgctcggc acggtgatca
     1981 acgcgctcgc cgtgcagagc gtcatcgagc aggccgggct cgccacgcgg gtcatgtcgg
     2041 ccattcccat gtcgtcgatc tgcgagcctt atatccggcg ccgggcgatc cgccatctgg
     2101 agaaggggcg ggtggtgatc ttcgccgccg gcaccggcaa cccgttcttc acgaccgaca
     2161 cggcggcggc cttgcgggcg gcggagatgg gctgcgacgt gctcttgaag gcgacccagg
     2221 tggacggcgt ctacgatgcc gacccgaagc gggacaagac cgcgacgcgc ttcgagcggc
     2281 tgagctatct cgatgtcctg gccagggacc tgcgggtgat ggacgcgtcg gcgatctcgc
     2341 tggcgcgcga gagcaacatc cccatcctgg tcttttcggt gcatgttcca ggtgccctgg
     2401 cgcgggtgct gcgcggcgag ggcatgttca cgatcatctc gggtgaggcg gcagcatgag
     2461 cgatggagag ccggatatcg acgacctgac gcggcgcatg gacggtgccg tcgaggcgct
     2521 gcggaaggaa ttctcgggcc tacggaccgg ccgggcctcg gcaagcctgc tcgagccgat
     2581 cacggtcgac gcctacggcg cgtcgatgcc gctcaaccag gtgggcacca tcaacgtgcc
     2641 ggaaccccgg atgatctcgg tccaggtctg ggacaagggt ctcgtcaagt cggtggagaa
     2701 ggccattcgc aactccgccc tcggcctcaa cccgcaggtc gacggcacgc tgatgcggat
     2761 tcccctgccg gagctcagcg aggagcggcg tggcgagctc gtcaaggtgg cgcatcgcta
     2821 tgccgagcag gcccgggtcg ccatccgcaa tgtccgccgc gacggcatgg accagctgaa
     2881 gaagctggag aaggatggcg ggctctcgca ggacgatcac aaggtctggg ctgacgagat
     2941 tcaggccctg accgaccggc atgtcgagct ggtcaacgac ttgttgcagc acaaagagaa
     3001 agacatcgtc accgtctagt cggtgacgcc agcagccgga taggacgtgt cagctccagc
     3061 gctccgctca gcaacacctg caaccggcgc ctcggcggcc ccggtccatg tcgcgatcat
     3121 catggacggc aatcgtcgct gggcgcgggc ccgcggcatg ccgccggttt tcggccatcg
     3181 gcgcggcgcc gatgccgtca ggcgggcggt cgaggcctgc ggcaatctcg gggttcgcta
     3241 tctgaccctc tacgccttct cgagcgagaa ctggcagcgc ccggcgggcg aggtcaccga
     3301 gctcatgaac ctgctccgct tctatctcag gaaggagatc gaggcgttgc acaggaatgg
     3361 cgtcaggctg cgggtgatcg gcgaccgctc caggctggac cgcgacatcc tcgagctgat
     3421 cgacaacgcg gaggcgttga cccgcgagca tcaggcgctc gagctgatcg tcgccctcaa
     3481 ctacggcgcc agggacgaga tcgtgcgcgc cacgcgccgg ttggcagctg ccgccgtggc
     3541 cggcgagctg gcgcccgaga ccattgacca cgggcagttc gccgccgcgc tcgacacctc
     3601 gggcgtgccc gatcccgacc tgctgatccg cacctcgggc gagcagcgga tcagcaattt
     3661 cctgctctgg cagctggcct acaccgagct ggtctttctc gacgtgcact ggccggactt
     3721 cagcgaggag catctgcgcc tggcgctcga ggagttcggc cggcgcgagc gacgctatgg
     3781 cgcctgcgcc ggttgaaggc agggtgcccg ccgatttcgc caagagggcg attgccggtg
     3841 tggtgatggc gctcgtcgcc gggctcgtga tcgtgctggg cggctggtat ctggtcgccg
     3901 tcctgatgct ggtcgttgtc ctcgccgccg gcgagtgggc ggcgctgacc ctgcaggaca
     3961 gccgcaagcg ctacgccgtg ctcggtatgg tgctgctgcc gctggcgatc ctggccggca
     4021 cccatcaggc gatcaccgca tggcgtggca ctgacatggc gctggtcggc ggcgcgctcg
     4081 gcgccgtcct gctcctcgtc agtggatggc tcatccgccg ggtcggcggc tggagcgaac
     4141 ggcgctgggc ggtgctcggc attctctatc tgggcgtccc ggcggttgct ctgctggcgc
     4201 tgcgcgatct gcccgacggg ctccatctcg tgctctggct ggtcgccgtc gtcgtttgca
     4261 ccgacgtctt cgcctatctg gtcggccgca cggtgggcgg cccgaagctg gcgcccagaa
     4321 tcagcccggg caagacccgg tcggggctgg ccggcggcat gctggccgcg gcggtcgccg
     4381 gggcgttgct cgccgggtgg gtcggctggg gctggttcca ggcgggcctc gctggcgccg
     4441 ttctggcact ggtggcgcag accggcgacc tcttcgaatc ggcgatgaag cggcgggccg
     4501 gcgtcaagga cagcggccac ctcattcccg gccacggcgg cgtgctcgac cggttcgatg
     4561 gctttctctt cgccgctccg gtcttcgctc tgctcgtcgc catcgcacgg acaggcgcct
     4621 aggctgatgg ccgcgtcccc cgtgcatcgg ccgccgagtg ccgcagcacc acggagcatc
     4681 accgtgctgg gtgccacggg ctcggtcggg cgcagcacgc tggccctcgt tcgggccgat
     4741 cccgccgcct accgggtgac agcacttgtc gccggcagcc gggccgcacc tctcgcagca
     4801 ctcgcccgcg agttcgacgc gaggctcgcc gtggtcgccg atcccgcggc ctatccggag
     4861 ctccgggcag cgctggccgg cagcgggatc gaggcggcgg ccggaaccga ggcggtgctg
     4921 gaggcggtga tccgccccgt ggactgggtg atgtccgcga ttgtcggggc cgttggcctc
     4981 gcaccgacgc tcgaggcgct gcggcgcgga cggatggtgg cgattgccaa caaggaatgc
     5041 ctggtctcgg ccggcggtct gctgctggct acggcgaagg caagcggggc cgtggtgctg
     5101 ccagtggatt ccgagcacaa cgccatgttc caggccttgg tcgggcatga ccgtgcggcg
     5161 gtggagcgac tgatcctgac cgcctcgggt gggccgttcc gcacgctcga ccgggctgcc
     5221 atggccaagg tgacggtcga ggaggcgttg aaccatccca actggtcgat gggtgccaag
     5281 ataaccatcg acagtgcgac gatgatgaac aaggggctcg agctgatcga ggcgcaccac
     5341 ctttttggcg tgccggaggc ccgcatcgag atcctgatcc acccgcaatc ggtgatccac
     5401 agcctcgtcg cctaccggga cggctcgcag ctcgcccagc tcggcgtgcc ggacatgcgg
     5461 gtccccatcg ccttctgtct cggctggccg gggcggctcg cagtcgaggg ggtgcagctc
     5521 gatctggcaa aggtcggccg gctcgagttc gcggccccgg acgcggagcg ctttccggcc
     5581 ctgcgcctgg cccgtgacgc gcttcgttcg ggtgggacgg tgccggctat gctgaacgcg
     5641 gccaacgagg tggcggtgtc ggcgtttctc gagcggcgga tcgggtttct cgacattgcc
     5701 gccgttgtcg agcaggtttt gatgcaactg ccgggtgacg aaccccgcac gatcgacgat
     5761 ataagggaat gtgacggcgt gactcgcagg gtcgcccgca gtgaagtcgc aaggcgtgcc
     5821 gcgcagcatg cctgagcccg gatggaaagc cgacccatgg atctgatcga cctcgtcgtc
     5881 aattatgccg tgccgttcct gatcattctc tcggtggtgg tcttcgtgca cgagtatggc
     5941 cactactggg tggcgcggcg caacggcgtg cgcgtcgagg tcttctccat cggcttcggg
     6001 cgtgagctct tcggcttcaa cgacagccac ggcacgcgtt ggaagttcag cctcataccg
     6061 ctcggcggct acgtgaagat gctgggcgac gccgatgcga ccagcgcgct ccctgatccc
     6121 agcaccgcct ggcaggagga gagcttcccg gcgaagcggg tgtggcagcg gatggcgatc
     6181 gtcgccgccg gaccggccgc caacttcctc ttcgcgatcg tggtgctgag cgtgctcttc
     6241 gttgccagcg gccggccctt caccccggcc gtggtcggcg aggtgcagcc cgagagcccg
     6301 gccgccgccg ccgggctcga gccgggcgac cggatcgcgg cggtcgacgg ccaggcgatc
     6361 gacagcttcg aatcgctgca ggctgcggtc cgcgacctgg cggaggtgcc gctcgagttc
     6421 accgtggatc gcgacggcgc caatttcacc gtgacggtcg tcccggccct gcgcgagatc
     6481 gaggaccggt tcggcaacgt gcatcgggtc ggcctcgtcg gcatcagccg caccggcatc
     6541 gagttccggc gcagcaaccc ggcctatgcg gtcttcgagg ccaccgccga gaccggccgg
     6601 atgatcggcg cgacgctcaa ggcgctgggc gagatggtgg tcggcacgcg cggcacggac
     6661 gagctcggtg gacccctgcg gatagcacag atgtccggga ctatcgccaa ggacggcctg
     6721 gtgccggcga tttggttcac ggcaattctt tcgatcaacc tcggattgat caatctcttt
     6781 cccatcccga tgctggacgg cggtcatctg gtgctctatt cgatcgaggc cgtgataggc
     6841 cggccactgg gtgagcgtac tcaggagatg gcgtttcgcg tcgggctggc gatggtgctg
     6901 accttgatgg tctttgcgac gtggaacgat ctcgtgcaac tgaacgtcgt caagttcgtg
     6961 accggcctga tctcctagga gcggccggat tgcggaccgg accgccggca tccgtcacgt
     7021 cgcggcgcca agcaagaaaa cgggggtagg ggatgaggcg tgcgctcggc gtgatcgtga
     7081 gcgtctgttt gttgctggcc gggtcgttcg ggcacgagag ccgggcccag gtcgaggacc
     7141 ggatcctgga tatccaggtc cagggcaacg agcgcatcga gacgctgacc gtcgaaagct
     7201 acctcaccgt gcgacgcaac gatccgttcg accctgatgc gctcgaccag tccttgcgca
     7261 acctgttcgc caccggcctc ttcgacgacg tggcgatcga gcgggtcggc tcgacgctga
     7321 tcgtcagggt ggtcgagaat ccgatcatca accgtatcgc gttcgagggt aaccggcggt
     7381 tggacgacga ggtgctggag gccgaggtgc agctccggcc gcgagtggtc ttcacccagg
     7441 cccgtgtgca ggcggccgtg acccgcatcc tcgagctcta tcggcgcaat ggccgctatg
     7501 ccgcgagcgt cgatccgaag atcatcgagc tcgaccagaa ccgtgtcgat ctggtcttcg
     7561 agatcgagga aggtccgctc acgaaggtcg cgggcatcag cttcatcggc aacgcctcgt
     7621 tctccgccaa cacgctgcgt ggcgtgatcg agaccaagga ggcggcctgg taccgcttct
     7681 tcagcagcac cgacacctat gatccggacc gcctggcgtt cgaccaggag ctcctgcggc
     7741 gcttctatct cgaacgcggc tatctcgagt tcctggtcgt cagcgccatc gccgagctga
     7801 cgcccgaggg cgaccgcttc ttcatcacct tcacgctcga ggaggggccg caatacaccc
     7861 tctcctcggt cgagctcgag agctcgatcc ccgatctgcc gctcgatgcg ctacgcgagc
     7921 tgatcacccc gaagtcgggc gacgtctaca acgcgaccga ggtcgagaag tcgatccagt
     7981 cgctgaccga ccggctcggc cagctcggct atgccttcgc cgaagtggat ccgatcaccg
     8041 agctgaatcc ggacgagctt ctggtcgacc tcatcttcca gatcaacgag gggcccaggg
     8101 tctatgtcga gcggatcgac atcgtcggca atgtccgtac cctggacaag gtgattcgcc
     8161 gcgagttccg gctcgccgag ggcgacccct acaatctggc cctgctgcgc cgctccgagc
     8221 agcgcatacg caacctgggc ttcttcgaga cggtgaacgt caggaccgcc cgcggcagcg
     8281 ccgccgacaa gatcgtcatc caggtggagg tggccgagca gtcgactggc gagctttcct
     8341 tcggtgccgg cttctcgacc gccgacggcc cgctcggcga catccgcctg gccgagcgca
     8401 acctcctcgg caccggacag agcgtctccg ccaacttcac gatctccggc cgccggcagg
     8461 acatcatcgt gagctacacg cacccctatt tcctcaaccg ggatctggcg gcgggcttcg
     8521 atctcttcct cagcgagacc gattaccagt cggagagctc gttcgacgaa gagagtgtcg
     8581 gcggcacgct gcgcgccggc tatccgctta ccgagaacct ccgacacggc gtgcgctaca
     8641 cgctgcgcga agacgagatc aggaacgtcg acaacgacgc ctcggtgttc attcaggacg
     8701 aggaggggac ctctgtcacc tcgctggtcg gccagacctt cacctatgac gtgcgagaca
     8761 cgcgcttcct gccgtccgag ggctatatct tgaggctgga ccaggatctg gccgggctcg
     8821 gcggcgacaa ccgtttcctg cgcaacgacg tgcgcgggga ttactactac tctatcttcc
     8881 ccgacgtggt cctcaacgtc ggtgccggtg tcggttacat ccatggactg ggagacgacg
     8941 tggggctctc caaccggttc ttcatcggtg gctcgtcgct gcgcggcttc gcttttgccg
     9001 gtatcggtcc gcgcgacagc gattccaatg acgcgctggg tggcaatctc tactataccg
     9061 gcacggccga ggtgcggttt cccctgggcc tgccggacga gctgcggata ttcggccgca
     9121 ccttcgtcga tgccggcacg ctcacggaaa tcgacgtcaa tggcccgacg ctcgaggaca
     9181 gcggcagcct gagggttgcg gtcggcttcg gcttttcctg gctctcgccg ctcggcccgc
     9241 tggcgatcga tctcgcccag gcggtgcgca aggagaatta cgacgagacc gagctcttcc
     9301 gcatttcgtt tggcacccgt ttctagctgg tggccgggca tgacggccgg gcgccggtgg
     9361 cggcagctcg ccctcaccgc cctgctcctg ctcgcctccg gcctgccggt gatggcggag
     9421 ccgctgccgc cggcgaccgc tgcggtgatc gactatcagc gggtgatgcg cgaggcgctc
     9481 gccgcccaga gcattcgtgc ccaggtcgac gcgcggcggc tgcgctacca gcaggagatc
     9541 gaggctgacc agcaacggct gcgcaacacc gatcgtgagc tgatccgtca gcgcgggatt
     9601 ctcgacgcgg atgcctatgc cgagcagcga aaggcgttcg aggaggaggt ggcgcaaatc
     9661 cagcgacagg tgcaggagcg cctgcgccag ctcgacgacg tctcggccat ggcgctcgcc
     9721 gaggtgcgcg aggcggtgat cgagatcgtc ggtgacctcg ccgaggtgcg cagcttcaac
     9781 ctcgtgctgc cgagtgccgg ggtgctcctg ttctcgcctc agatcgacat tacggacgag
     9841 gtgctggcag ctctcgacga gcgtctgccg gaggtcgatg tgccggagcg ggcgccgcaa
     9901 taggtgctgc ggtgatggcg ccacccggcc ctcggggccg ggtggcgaaa aggcttggcc
     9961 tcgccgcctt cctgtgctgg tttccggccc cggcgcgggt gccggtagtg tcaggaaagg
    10021 agcgggaagc gatgagcgag gccgcgagga agccggggcg ggcgatgccg gatatcgagg
    10081 tcggtcgcct gatgcaactg atcccgcacc gattcccgat gctgctgatc gaccggctga
    10141 tcgagatcga ggcgcccgag cgggcgaccg ggatcaagtg cgtaacgatc aacgagccgt
    10201 tcttccaagg gcacttcccc ggcgatccga tcatgcccgg cgtgctgatc gtcgaggcga
    10261 tggcgcagac cgcggcggcg ctggtgatcg cggggcagga gcgcgagggc aagggtgacc
    10321 tcgtctattt catgggtatc gaccaggccc gttttcgcag cccggtgcgg cccggcgacc
    10381 agctcaggct cgaggtggag ctgcagaagc accggctcgg catccactgg ttcaagggcc
    10441 gggccgtggt cggcggccag gtggccgccg aggccgtgtt caacgccaag ctcttcggtc
    10501 gcgagcaggt ggccggcggc tgagccgccg gccacatgtt cagccgcgcc ggtcgagcgg
    10561 cacgtaggag cgctcggcgg gaccggtata gagctgccgc ggccggccga tcttctgctc
    10621 cggatcctcg atcatctcgt tccactgggc gatccagccg accgtgcggg cgagggcgaa
    10681 gatggcggtg aacatcttgg tcgggatgcc catcgcctcg aggatgatgc cggaatagaa
    10741 atcgacattg gggaacagct tgcgttcctg gaagtacgcg tcctcgaggg cgatccgctc
    10801 gagctcgcgg gcgatggcga gcttgggctc gccacccttg cccaactcgt ccagcacctc
    10861 gtcggcgctg gccttgagca cgccggcgcg cggatcgtag ttcttgtaga cgcggtggcc
    10921 gaagcccatc agccggaacg gatcgtcctt gtccttggcc cgggcgagga actcggggat
    10981 ccggtcgacg gtgccgatct cctcgagcat attgaccacg gcctcgttgg cgccgccatg
    11041 cgccggcccc cagagcgagg cgatgccggc cgagatcgcc gcataggggc tggccccggt
    11101 ggagcccacg agccgcaccg tcgaggtcga ggcgttctgc tcgtgatcgg catgcaggat
    11161 gaagatcgtg tccagcgcct tggagagcac cggcttctcc ttccacggct cgcacggcac
    11221 gccgaacatc atgtagagga agttctcggc gtaattcagg tcgttgcgcg ggtagaggaa
    11281 aggctggccg accgagtact tgtaggccat ggcggcgatc gtcgggatct tggcgatcag
    11341 ccggtagctg gcgatcagcc ggtgcttcgg gtcctgggta tcgaggtcct cgtagtagaa
    11401 ggccgagagg gcgccgacga ccgccaccat gactgccatg gggtgggcgt cgcgacggaa
    11461 gccgcggaaa agataattga tctggtcgtg gatcatggtg tggttggtca cgccgtccac
    11521 gaacttcttg tactcgtcgg ccttgggcag ctcgccgaac aggatgaggt agcacgtctc
    11581 gaggaagttg ctgcgttctg ccagctcctc gatccggtag ccgcgatgca ggaggaggcc
    11641 ggcctcgccg tcgatatagg tgatcttcga gcggcaggac gcggtcgagg tgaagcccgg
    11701 gtcataggtg aagtggccgg tcgccgagta gagcttgcgg atgtcgatca cgtccgggcc
    11761 gatcgtcccc tcgagcacgg ggaactccga ctgctggttg ccccgcccga tcttcaccga
    11821 tttttctgtc gtgctctcgg tcatcgcgtg tctccttggc tttcgcgcgg cgctggtggc
    11881 cggtttgcgc gcattggcgg ggctgccgtc cgtcgtccgc tgccgcgcga atggggcgtg
    11941 cggtcgccgc gtccggctcg atgcttcctc gacggtcggc cgactgtaga gcccgggagc
    12001 cgcggcttca atcgctctgc tgcattgcgg gattgcgacc agtcgcagca tcctcgagcc
    12061 gcagcagcac ctcctcgcgc ccgaggatcg ccatgacctc gaaaaggccg ggcgaaaccg
    12121 tgcggccggt gagggcagcg cgcaacggct gggccagctt gccgaagccg aggccttgcc
    12181 gttccgcatg ggcgcgggcc gccgcctcga gcccggcctc ggcgaagggc tcgaccgctg
    12241 ccagcaccgg ggcgagtcgg gcgagctcgg cccgggcggc cgggtcgagc agcttcgccg
    12301 ctttggcgtc gagcggcagc gggcgcgcct ggatatagaa ggcggcgctc gaggcgaggt
    12361 cgaccagcgt gcgggcgcgc tgcttgagcc cgggcatcgc ctgttccagg cgccggcgct
    12421 ccggctcgcc gagccgatgg cccgctgcct cgagccgcgg tgcgacgagt gtcacgagct
    12481 cgttgtcggc ccgctccttc aggtagtggg cgttcaccga atcgagcttg gcgaaatcga
    12541 agcgggccgg cgagcggccg atgccgtcga ggtcgaacca ggcgatcgcc tgctgggtcg
    12601 agatgatctc ctcgtcgcca tgcgcccagc ccagccgcag caggtagttg cgcatggcct
    12661 ccggcaggta gccgagctcg cgataggcgt cgacgccgag ggcaccgtgg cgcttgctga
    12721 gcttcgcgcc gtcggggcca tggatcagcg ggatatgggc gaagaccggc ggctcgtggc
    12781 ccagggcccg gaagagattg gcgtggcggg cggcgttgtt gaggtgatcg tcgccgcgga
    12841 tgatgtgggt gatgcccatc tcgatgtcgt cgaccacgac ggagagcatg taggtcggcg
    12901 tgccgtcggc gcgcagcagg atcatgtcgt cgagctgcgt gttggcgaag cggacagtgc
    12961 cctggacccg gtcctcgacc accgtctcgc cgtcgagcgg cgccttgaag cgcaccaccg
    13021 gatcgacgcc ggccggcgcc tcgcgcgggt cgcgctcgcg ccaggtgccg tcgtagcgga
    13081 tcggccgctt ctcggcccgt gcccgctcgc gcatcgcctc gagctcttcc ggcgtgcacc
    13141 agcagcgata ggcgagaccc tgctccagca ggcgctggac cgccgccttg tgctgctcga
    13201 tccggctgaa ttggtagact ggcggctcgt ccgactcgag gccgagccag ccgaggccgt
    13261 cgaggatcgc cgcgatggcc ggctcggtcg agcgctgccg gtcggtatcc tcgatgcgca
    13321 gcaagtagcg gccgccatgg tgccgggcga acagccagtt gaagagggcg gtgcgggcac
    13381 cgccgatgtg cagatagccc gtaggtgaag gagcgaagcg gacaataaca ctcatggttg
    13441 atttttcgcc ggaattcgag gaggagatga aaggatacgg cgcggcctcg taacacgatc
    13501 ggaacggact tgcagcagcc ggccctcgtg ccatccgcca gatcctgggc gccacgcccg
    13561 gcgggcggct ccaggctgct cctggcgttg gttgctgtcg tgctggagtc gctgcatgcc
    13621 gagcggccac gctggccgct ctgggcgccg atcgcttttg ctgtcggcat tctcgtctat
    13681 ttcgccctgc cggtcgagcc gtcgccgagg ctgctggccc tggtaccgct cggggtgatc
    13741 ggcgccatcg ctggtgcgcg acgggccgcc ggcctcgccg ttcttttctg gctcggcctg
    13801 gccctggcgc tggccggctt cgctgtggcg cagctgcgca cgctccaggt ggccgccccg
    13861 gtgctgccgc gctccggcgt ttaccatgtc gagggggtga taagggccgc cgagccgcgc
    13921 aacgacggcc agcgcctgct gctcggcgat ctcgtgatcg agcgcctgga agaggcggcg
    13981 acaccggccc ttgtgcgctt caccgtgcgg cgcacggagg tgcccttcct gccgggtcag
    14041 cggatcaagg cgcgtgcccg cctgcagcca ccgagtggtg cggcggtgcc gggcggcttc
    14101 gatttcgcga tgcgggcctg gttcgagcgc ctcggcggtg tcggcttcgc gctcggggcg
    14161 cccgagctgc tggcggccgg tccgggaacc gggacccgac cgatcgccac cctgcgtcat
    14221 gccgttgcgg ctcgcgccga gcggatcatc ccgggcgccg atggcgctgt ggccgcggcc
    14281 ctgctgacgg gcctgcgcgc ctccataccc gaccgggtgt ggcacgacat gcaggcgagc
    14341 gggctcgccc atctgctcgc catctcaggg ctccacatga cgctgatcgc cggcaccgtt
    14401 ttcgtcgtcg cccgcgccct cttcgccttg tggccggctt tggcgcttcg cgtggtgccg
    14461 aagaagccgg ccgcggtcgt ggcgctcctg gcagccctcg gctatctgct gctcgccggt
    14521 gccaccgtgc cgacccagcg cgccttcatg atggcggcgg tggcgctgct cgccgtgctg
    14581 ctcgaccgca acccggtctc gatgcggctc ctggcgatgg cggccttcgc cgtcctggtc
    14641 ctggcacccg agagcctgct cggcccctcc ttccagatga gcttcgccgc ggtcaccggc
    14701 ctcgtcgcct tctacgagct gcgcctgcgc cggctcgccg ccgcgccacc ggactccgac
    14761 cggcagcggc ccggccgcct gctgctggtc tatttcgtcg gcatcctggt caccacgctg
    14821 atcgccagcc tcgcgaccac gccctttgcc gcctaccact tccagcgagt ggcgacctat
    14881 ggcgtcctcg ccaatctggt ggcggtgccg ctcaccgcct tctggatcat gccggccggg
    14941 ctcctggcgc tgctgctcat gccattcggg ctggatgcac ccatcctgca tgtgatggga
    15001 gcaggaatcg gcatcgtgct cgacgtcgcg gctttcgtcg cggcgctgcc cggcgccggc
    15061 atcgacgtca gcccttggcc gccctcgagc ctgctgctgc tggcgctggg cgggctctgg
    15121 ctttgtctct ggcggcagta ttggcggctc tggggggggg ctgcctgtac cctgggtatc
    15181 gtcctggccc tgaccacacg gccgccggac ctcctggtcg accgacgggg cgatctcgtc
    15241 gtctatcgcg ctgccggcgg cgaagttcac ctgctcgagc tgaagcgcga ccgctggctc
    15301 gccgagcagc tcgtgcgagc cgccggctcc ggcgaggcga gcgccttcga aacacggtcg
    15361 gacgggcagc tggcctgcga tccgctgggc tgtgtcgtcg gccccgagcc ggcccgccgg
    15421 gtggccatcg cgctgaccgc cgaggcggcc gcggaggatt gcgaccgggc ccggttcgtc
    15481 ctgctgctcg ccggcccgga gagctgcgcc aatggcacgc cgagcctggg cgcgcgcacc
    15541 ctctggcgga gcggcggggt ggcgctcacg atcgcggacg ggcgggtgcg ggtcgaaacc
    15601 gtggccgact gggcgggcga ccggccatgg atgcgctgag gcaccattgc ggttgaatcg
    15661 atttcaactt atgatacaat taaattgaat tgtattgcaa ccaggaggct aggccgatgc
    15721 tcgcccaccc gacgcatccc tttgcccgga tcgacacgct gcgccaccac gctctggctc
    15781 gtccgctcat ggcggcactg ctcctggtgg tcggcggact gctgctctac tcggtggcgg
    15841 cgctcgtcga ccggcgcgat gccgccgcca ccttcgtgcc cgggagcggg atcggactgc
    15901 tcggcagcgg tggcgttcag gcgctgacat tgcccttggc cacggagcgg cagccagccc
    15961 gggagccgac tccacggcct tcggcgaccg attccatcgt ggccccggtg ttggcggctg
    16021 aggtggccga cgatctcaag gtgatgctgg ccggaacgtt ggagcaggtg gctccggccg
    16081 ctggtatgcc ccgtccgccg ctggacgcgg cggcaccgcc cttgcgggtc gagatcatgg
    16141 gactcgccga taccgcatgg acgtcggtcg ccacgaccaa tcggtagccc aggccgatcc
    16201 cgtcatgcat ccctcgcatg cggcccatcc ggcggttggc ccgccgccgg aggcgggtcg
    16261 cgagctttcc ctgccggagc tgctggcggt gctccgccgt tatcggctgc tgctcgctgg
    16321 cgccctcggc gtcggcctcg gcatggcggt gctgctcctc gcctggctgc cgccggtcta
    16381 tctggcgacc gcccaggtga tgatcgagcc gatgcgtcaa gggccggaag acagtgccct
    16441 gcctgccgcc ggcatcgccg acgacggcac ggcgatcgac agccaggtca agctgctggc
    16501 ctcgcgcagc atggcgcgcc aggtgatcga ggcgatgggg ctcgagacgg agcccgagct
    16561 gcaacgctcc ctcgacgtgg cggagcggct cggcgacctc gccggcgtcg cccgggcagc
    16621 cgctcgcgag gacgatgcca tcggcctcat cgaccgcttc ctcgagcgcc tcgaggtggc
    16681 gcgcgacggc aagacccatg tcatccgcat cactttcagg agctcggatc ccggcgtggc
    16741 ggcccaggtc gccaacgggg tggccgaacg ctacattgcg ggccagctcg aggccaaggc
    16801 gcgggcgacc cgggatgccg ccgactggct gcgcacggca ctcgactcga gccgcgaggt
    16861 tctggaggtg gccgaaagcg agctcgccag ctttcgtgag aatgtccagg acgactacgc
    16921 cgatgcgttc gccctgcatg gcgtggacgt ggtcaacctc cgccgcgacc tcgtggcggt
    16981 cgcgggcgag cgcgccgcca ggcagtccga gctgggtcgg ctgcgccgcg tgctcgcgga
    17041 gaacaattcc gatctcgcct tccaggagct cggtggctcg gaggtcctcg aggacctcta
    17101 caacctgaag aaccagacga cccggcgcga ggcggagctc gccacgcagt atggcgaccg
    17161 gcatccgctg atcctcgacg tgcgcgccga gcggcgcgag ctcgagcggc gcatccgggc
    17221 ggagcagctc gctttggtcg gcagcgtcga ggcagagatc gcggcggctc gggcacgcga
    17281 ggcggcgctg cagcgggagc tcgaggggct gaagagccag aacagcgcac tggctcaggc
    17341 ggaagcgcgg atcgccgatc tcgaacgcgc ggccgatcgc gcccggcgcc tgcacgaggc
    17401 ctatctggcg cgttaccagg cggtggccga tgccggcgac acgcagcgcc cggacgcccg
    17461 gctgatctcc gaggcgaccg tgccgccgag gccgagccaa ccacgaccgg ccctgatctt
    17521 cagtcttttc ggggcggcat cgttgggcac ggggctgctc ctggtcttct tcctcgagca
    17581 gctcgaccat ggcttccgca gtgtccgggc gctggagcag gggttggggc tgcgctgcct
    17641 cggcgccgtg ccgctcgtcg accggcgccg ccggcgcggg ctggcgctgg ccgacctgcc
    17701 gctcgaacgg ccccgatcgc gattcgccga gagtctgcga gcgctgatcg cgggcctcgt
    17761 cggcccggcg gtcgagggtg ccgggcgggt cgtcctcgtc acctcgtcgg tgccgggcga
    17821 gggcaagtcc acgacgacca ctgccctggc gcggctcgcc gctgccgaag ggctgaaggt
    17881 gctgctgatc gacgccgatc tccgtcggcc gatcctggcc acactgcttg gcctcgaggg
    17941 ggagacgggg ctggtcgaga tcctgcgcgg cgaacgcgag gcggaggccg tgctgcgggt
    18001 cgaccggcgg agcgggctga gcttcattcc gggcagtgcc cgggtcagcc agccgacccg
    18061 gcttctcggc cccgccgcca tgggcatcct cctcgatgag gcgcgccgcc acttcgacct
    18121 ggtgctcgtc gacagcccgc cgctgctcgc ggtgggcgac gcccgggtga tggcgccctt
    18181 gtgtgacgcc gtgctcctgc tggtccgctg gaacgccacg cagcaggccc tggcgaccca
    18241 tgcgctgagc cagctcggcc aggcaccggg acggccggtg ctggccgtgc tcacgggcgt
    18301 cgatccggat gcgcagctcg gcctcggctc cggcgaatcg cgggtggtgc ggcgccagct
    18361 cctccggtac tacgccgagg gctgatccgc ccccaccttc ggcagtccca tgccatgacg
    18421 ttcaacctgc gcttcgcgcc gagcccgacc ggcagactcc atctgggcaa tgcccggacg
    18481 gcgctggtca actggctcca ggcccgccag gcgggtggcc gcttcgtgct ccgcctggac
    18541 gacaccgata ggcagcgttc gaccgaggcc ttcgcccgga gcatcaccga tgatctcgcc
    18601 tggctcggca tcgactggga tgtctgcatc aggcagagtg agcgcgcggc gttatatgcg
    18661 acggcttttg ccggcctgcg ggaacagggg ctggtctacc cggcctacga gacggccgag
    18721 gagctcgcgg acaagcgggc ggcgcagcgc aggctgggcc agccgccgcg ctacgaccgt
    18781 gcggcgcttg ctctcgatgc ggccgatcgg gcgcagctgg agggcgaggg ccgggctccg
    18841 cactggcgct tccggctgag cggggctgca attgcttgga ccgatctggt ccagggatcg
    18901 gtctcgatcg ggacggcggc gctgagcgat ccggtgctgg tcagggcgga tggcgtcgcc
    18961 acctacacgc tggcctcggt ggtggacgat ctcgaatgcg gtatcacgca gatcgtgcgg
    19021 ggcgacgacc accggaccaa cactgcggtg cagatcgagc tgatgcgggc gctgggtggc
    19081 atgccgccag tcttcgccca tctgccgctc atcctgggtg cgggcggggc accgctctcg
    19141 aagcgcagcg gcgattggag cctcggcgac tggcgggcgc gcggcatcga accgcatgcg
    19201 atccgcctct atctcgcgag cctcggcacc gaccgcgtgg caccgccgga gaccagccgc
    19261 gaggcgctga ttcgggagtt cgatatcggc cggttcggac gagcgggcgc cgtcttcgat
    19321 gcggaccagc tcgcgcggat gagccgggcc gtctttcgcg cactgccgct cgaggcggtg
    19381 cgtgaccggc cgggctgcgc cgggattgct gccgccgagt gggcggccct tcgcccgaac
    19441 gtggcggatg ccggggagct ggcccgctgg ctcgccgtgc tgcaggaacc gatcaagccg
    19501 gttcgcgagg acgccgagct gctcggcgcc gcggccgatc tcctgccgct cgcgctcgac
    19561 gatgcatcgg cggcggcctg gctcggtgct gtgcaggaga gcaccgggcg acgcggcaag
    19621 gcactctacc acccgctcag gctggcgctc accggccgca cggatggacc caagctggcc
    19681 gatctgctac cgctcctcgg ccgtgagcgc gttctccgcc gactgcgcgg cctgaccgcc
    19741 tgacacacct gtcccgtgag cgagttgccc gagccttgac cctcgtcctc cacaatacgc
    19801 tgacccgcgc cgaggagccg ttctcgccac tcgtggccga gcatgtgcgg ctttatgtct
    19861 gcggacccac ggtctatcag cgcatccatg tcggcaatgc gcgtccgctc atcgtcttcg
    19921 acgtgctctt ccgcctgctc tcccatctct atccgcgcgt cacctatgtg cggaacatca
    19981 ccgatgtcga cgacaagatc atcgagcagg cacgggcgaa cggcgagccg atcgaggccc
    20041 tgaccgcccg caccacggcg gccttcctcg aggatgcggc ggcgctcggc tgcctcgagc
    20101 cgacgatcca gccgaaggcc accgaacaca tccctgagat ggtggcgctg attggtcggc
    20161 tgatcgagcg gggccacgcc tatgcggccg cggggcacgt actgttcagc acgccttcca
    20221 tgcctgccta tggccggctc tccggccgca accgcgacga ccagatcgca ggggcccggg
    20281 tcgaggtcgc cccgtacaaa cgggacccgg ccgacttcgt gctctggaag ccggccgcgg
    20341 gggacgagcc gggctggccg agcccctggg gcagggggcg gcccggctgg catatcgagt
    20401 gctcggccat ggcggagaag catctggggc tgccgttcga catccatggc ggcggccagg
    20461 acctgatctt cccgcatcac gagaacgaga tggcgcagag ctgctgcgcc catgggctcg
    20521 agaccatggc tcgctactgg ctgcacaacg gcttcgtcga catgcgcggc cagaagatgt
    20581 cgaagtcgct cggcaacgtg ctccccatgg acgacgccct cgccgccgcg ccgggcgagg
    20641 cgatccggct gtggatgctg ggcgcccact accgtgcccc catcgattgc agcgaatccg
    20701 gctttgccga ggcgcggacc cggctcgatc gcttttacgg cgccctcgag cgggccggcg
    20761 agacgaccgc cgatgtgccc gatccggagc tgatcgccgc tctcgaggac gacctgaaca
    20821 cgccgaaggc gctggccgtg ctgcatgagt tgctgcacga gctcaaccgc gcctcgcaca
    20881 gtgcggcacc ggccattgcc ggccggctca aggcgtcggc tgggctgctc ggtctgctcg
    20941 aagcaccgcc ctccaagtgg ctgcgcggca cgctcgacga cagccggatt gccggtcttg
    21001 tcgccgagcg gcaggcggca cgacaggccc gcgatttcgc ccgggcggat gcgatccgtg
    21061 acgagctggc tgccgagggg atcctgctcg aggacggacc gggtggcacc acctggcgca
    21121 gcggccaatg accgagcggc tccggctcta cgacacgacg ctgcgtgacg gggcgcagac
    21181 ccagggcatc accttcagcg ccgccgacaa gcggcgcatc gcgacggatc tcgatgcgct
    21241 cggcatcgac tacgtcgagg gtggctggcc gggcgccaac ccgaccgacg atgccttctt
    21301 cgcgaccccg ccggcgctgg gtcgcgccaa gctctgcgcc ttcggcatga cccgccgggc
    21361 gggacgctcg gccgccaacg acccgggcct gcagccgctc ttccaggcga aggtgccggt
    21421 gatcacgctg gtcggcaaga gctcgaggcg ccacgccgag ggggcattgg gcgtcgacgg
    21481 cgacgagaat ctgcggatga tcagcgagag cctcgtcgaa agccgccgcc acagcgagga
    21541 ggtgatgctc gacgccgagc atttcttcga cggcttcaag gccgacgagg cctatgcgct
    21601 ggcctgtatc gaggcggcgc tcgaaggcgg ggccgactgg atcgtactct gcgacaccaa
    21661 tggcgggacg ctgccgcaag aggtgacccg catcgtccgc cgcgtggccg ccctggtgcc
    21721 gcgggagaag ctcggcatcc acacccacga cgacaccggc aatgcggttg ccaacaccct
    21781 catggccgtg ctcgaggggg cgacgcaggt gcagggcacg ctcaatgggc tgggcgagcg
    21841 ctgcggcaac gccaacctcg tctcgctgat cccgacgctg aagctgaaga tgggctacga
    21901 tgtcggcatc gacgatcagg cgctgacccg gctgacagcc ctcagccgcg gcttcgacga
    21961 gcggctgaac cgcacgccca gccggggtgc cgcctatgtc ggtgccaacg ccttcgccca
    22021 caagggcggg ctgcacgcct cggcggtgag caaggacccg cgctactacg agcatgtcga
    22081 cccggccttg gtcggcaacc agcgcgatat cctggtctcg gaccaggccg ggcgctccaa
    22141 cctgatgcag cgcttcgccg agatggggct cgagatcgag cccgcccccg acgaggtggc
    22201 agccctcctc gccaccatca aggagcgcga ggcccggggc ttctcctatg acggtgcttc
    22261 ggcgagcttc gagatcctgg tgcgccgcac gctcggccgg ctcgccgacc atttcgagct
    22321 cctgagcttt cgggtgatcg acgagcgccg cttcaacgcg gtcggcgagc tggtcaccca
    22381 gtcggaggcg accatcaagg ttcaggtgga cggtcgggat ttctacacgg tcgccgacgg
    22441 caacgggccg gtcaatgcgc tcgaccatgc actgcgccag gcactggccc cgacctaccc
    22501 gacgctcgcc ggcatggagc tggtcgacta caaggtgcgc attctctcgc ccgaggccgg
    22561 cacggcggcg gtgacgcggg tgatcatcga atcggccgac gacgccggcc gggtctggca
    22621 gaccatcggg gtctccggca acatcatcga cgcctcctac aacgcgctcc acgacgcgat
    22681 cacctacaag ctcctgctcg atggccttgc cgaacgctga gatgacgagc gggcccgaca
    22741 atgcgccgcc ggtcgccttc aagcggacca aggcggcgcg cggcggcatc gacgcgctcg
    22801 aagctgcgcc cgtcgtcatc ctcgtacgcc cgcagctcgc ggagaacgtc ggcacgaccg
    22861 ctcgcgccat gctgaatttc ggtctgggcg agctgcggct ggtggctccc gacttcggct
    22921 ggcccaacgc caaggcggtg gcggcggctt cgggtgcggc cgagatcctg aaccggatgc
    22981 agatctttcc gaccgtggca gaggcggcgg ccgacctgca ccacctctat gcgaccaccg
    23041 cgcgcgaccg cgggctcgtc aaggagcagc acgacccggc gagtgccgcc gccgcggccc
    23101 gggcacaggt ggcggcgggg cgccgggtcg gcctgctctt cggccccgag cgcaccggac
    23161 tcgacaatga cgagctggcg ctggccgacg gccttgtcag gatcgacgtc aacccgctct
    23221 ttccgtcgct gaacctcgcc caggcagtcc tgctctgcgc ctatgaatgg cggcggcagg
    23281 gggccgagcc ttcggccatg acggcaccgg agccggcgcc caaggcggcg ctcgacgggc
    23341 tcctgcgaca ccttttcgtc gagctcgagg cggccggcta cttccgctcg ccggaccggc
    23401 gggactcgct cggccgggcg atcgccgcca tggtcgagcg gatgcgcctg accgcacccg
    23461 aggtccagct catgcgcggc atcgtcaagg agctgcgccg ggggccgctg aagcggcctg
    23521 gcgacgacgc cggctagcgt tggcgggtca gtgtctgccg agcactgcca ggacatcgcg
    23581 cgcctcatgc aaatgccgcg tggcgtcggc gagcacgcga tcggtatcgc cggcgaaatc
    23641 gggcaggccg gcgagaagcc cgagcagggc ggcgtcacgc tcgctcgaca gccgttcggc
    23701 atggcgcctg gccagtctgc gaacctcttc ggcgtcgccc agttgccggg cggcggtcgc
    23761 cagcatcagc acgatttcgc cggcttccgt gtcgatcggg cccgattccg gcagggcgac
    23821 ctcgagcggt gacgcggcga cgcggaccgc aggccagtcg ccggcgtcga aagcggcgcg
    23881 cgcccgcagg agcccggccg caggcccgtc cgtcaggaca gtggcggtgc tgttggcggc
    23941 cagcgcctcg gccatgagct gctggcgatc ggcggtgagc tcggcgtcga ccgcgccgag
    24001 cggcgtgctg tcgacgagcg tcgccagcgc gcggtcgggt gctcccgagg cgaggtgcag
    24061 tcgggccagc atgagcccga gccgcgcccg tctggcgtcg cgctcggcat ggaggccgag
    24121 ccggtcgtcg aggatcccgg tgccggcggt cgcgaggccg gtggcgagca gcgccgcgct
    24181 cagggtggcg gccgcctcgt cgccttccgg cccgaccggc atcagctcgg cgaaccggcg
    24241 gtagaggagg agggcggagg tcggcgagcg ctggccggcc gcaagctcgt ccagggcttc
    24301 ggcgaaaaac gcggccattt ccctggtgat ggcctgagcc tcggcggcgg gcggtgagcg
    24361 atgcatggcg gtccgcaacg tatccagcgc ctcgtcaatt cggccggcct cctgctgcag
    24421 cccggcgagg cgccgccaga gggtgaattc ctcctgctga ccgcgccagt gccggcgatc
    24481 cgcctcgagc gtggcgatcg cctcgtcgat ggagatctgc ccggcggcca aagcgagtgc
    24541 cgtggccgcg gcacgggcga ccgcccgctc ctcgagcggg ccattccccg ccagcgcctg
    24601 ccaggcggcc agcgcctcgt cggtcgcacc gtcgcgctcg agggccgtgg cctcgagccg
    24661 gcgcaggctg cgcaaccggt ccgggcgaag atcctggacc gtcagccggt cgatcagctc
    24721 gaaggcggca tcggcccggc cggtctccag cagcagtcgg acacccggca aagcaagcgt
    24781 tgcctggaga tccagagggt aggtggcgag atgggcgaag ccccgctgcc agtcggcgag
    24841 tccctggtcc caggcgcccg tggccgctgc tgcggcgcca cgccagagcg ctgtttcgtc
    24901 gtcggcagcg agctttcggt cggcgagctg ttcggccgcc tcggccggac ggtccatgag
    24961 caggagcgcg gcgcccgtca gggccgcctg cgcggggggc gatgtcgatc cgggctcagc
    25021 ggtctcgaca gtcgcggcat cgagcagctc gagcacctca gcgccgagac cgttcacgac
    25081 gagaagccgg gcaaggtcga gacgggctgc ttcacggctg ggcctgtcgg cggtcgtgag
    25141 tgcagtcagg agcgctgccc ggcgtgtcct gaaatcatcc tccgcgaacc gggcgaggcc
    25201 aagcggtgac ggtggcgaat gcgcctcggc ccgatgctcg cctggtggtt cgtgatgagg
    25261 cacgggatgt ggcccagcgt tgtcgtccgc tgcgtgcgag tcgaccggca gccgggcgga
    25321 ttcctccatc ggcaggtgag cgaaggtcgg ctcggtcgag ggggcggcga cgacggccgg
    25381 tggtgcggcg gtcggcagga cgccatgggc gggagccagc agcaggccgg ccgtggtcag
    25441 gtcgtccggc tcgaccggct ccgccagcgc ccgccacacg gcaccttggg ccgagggcag
    25501 gagcctcagg ccgacgaagc gggattcgac caggcgcggc ccggtcggcg ccagggcgag
    25561 gccgacgccg agatgatcgc cgatcacggg gtcgaccagc gaggcgacgg acgtgatgcc
    25621 gggcagcagg aggccgtcgg cctcgggcgc cagtgccggg gcaccagtcg cgccggctgg
    25681 ctccctgggt ggccgcaagg tcaccaccca gctcgcatcg tcgcgatgga cgagcgccga
    25741 ccgctcgtcg gtgagggcca ggcggaggac ggtggcggac ccgtgacgct cgcggcgcaa
    25801 tccggtgata tgctgcccga ccgctgtcgc gaaggcctcg gtgtcgatct cgatggcgtt
    25861 cgcctgcgcg ccaaagacgg cccacaggct gcggccacga acaaagatgg cggcgggcac
    25921 cggggcggcc cagcggaagg cgatctcggt cgaggcgacg ccccgccgcg tgccggtgat
    25981 gacgacagga ccctcgacgg gcaacgccgc cggtgccacc ggcgcgtcgc cgaggtcggt
    26041 cgctgcgtgg acgatcgggg ccttctcggc cggcatgccc gtgtcggcaa cgaccgccgt
    26101 tggcgtgggt ctggcatggg ggagcggaaa gtttggactc gcggggctca cgacggtgtc
    26161 ggcgggagcg ccgatggcgt ctggcggtgg ggtgacgtcg atgaccagct tgtccggttc
    26221 aaccagcagg tcgcgggcgg tggcggtcgc cgcgaggccg agcgccacgc ggtttccgtc
    26281 gagcgttaca ccggtgatcc ccggcacctc gcgcagtcga ccgagtgccc aagcgctgag
    26341 tgggccgggc aggtccagct cgagcccggc ggggcttcgt tgcagcaggc tggcggcagt
    26401 ggcgaccggc tcgatcacaa ggcggacgaa acccggatgc tggccgaccc gcagccgaag
    26461 ctcggtcggc gcggcgcgaa aatcgaggac cagaaggcgc tcgtcgagca gctcgaaggt
    26521 gagatcgacc ccgggcgtcg cctcgaccgc gaggccggca ccgtcggcat cgaccttgag
    26581 cgctgcgagg tagcgatgga gccgtccgct caactcgctg agccgggccg ggaaggggtg
    26641 gctcgcctgc accttcagcc cggcaccctc gcgcgcggtc tggatctcga ccggcgcagg
    26701 ccattcgatc accagccggc catagttgtt atgcagcccg atgcggacca ttgcctctgc
    26761 cgcccgggca acgtctggca gaaggccgat cccggtatcg agaagcgccc agaggacgag
    26821 gaggaggcgc cagctcatgg tgggttggtc aacgtgtctc ccggcggcgg ctgggtgcgc
    26881 agggccaggc gcagccgatc gccacccggc tcgcccgcgg cggcgacgac cacctctgca
    26941 tcgaccaggc ccagctcctg cgtgagccgg tgctgaaatg ccaggacgcg gtcgagccga
    27001 gccggcagcg tctcatcgac tctggtcggc aggcgaaagc tcgcctgcaa cgcaaatctc
    27061 cgggcgaggt aggcataggt ggccatgagc tcggtcagag cactccgctc ggccgacgac
    27121 gcggaagatg ccggatcggc catgtcaatg ttaagttgaa attcagtgta gcttatattc
    27181 gcactggttg ccaagtctcc ctgctcgcgg agcagcgccg ccagataggc gaaggatcgg
    27241 tcggcctcgg cgctgatctc gggcgcggcc cgctccgcta cggtgccctg cattacgagc
    27301 gcgatcaccg gccgatccgg gtcgatcggc atgagcccgg tcgctggtcc gccgtcgagc
    27361 tcgcgcatcg agaggccgag gacgaagaag gtgagcagca gggccaccac atcggccagc
    27421 atgatcgacc aggctcgctg gccggcgagg tgccgctcag cccgcatcgg ctcgcctgac
    27481 ggccacacgc ggcacggcga aacgccaggt tccgggggcg agggcggcga agccgagact
    27541 gagtgctgcg ccggcgagac cgttggccgc gagcacttcg gcgagagcgg cgaggcggtc
    27601 ccgggcggga tcgcccaccg cctcgggcca tgcggtggtc accacgagtg gcgtgtccca
    27661 ggcggcgtcg cggccggcga gcgcgatagc gaggcggcgg agcagcacga ggcgttcccg
    27721 ccggacggcg ttgcccggtg atgcgaagag atccccttcg ccgagttcga aggcgatgag
    27781 catgccgggc agctcccggg cgctggcctg cacgggcagc gaggcggcga gccggcggcg
    27841 caactgctgc tcggcgcgga ccgaaccgtc caggggctcg ttgtcggcgg cgctgccgga
    27901 ggggccggca aagcgatcgg ccagcgcctc gagcaccggt ccggccttgg tggcgctgat
    27961 ctccgagcgg ctgtagagca cgacgaaaaa ggcgagcaga atcagcatca gcttgagaaa
    28021 gccgtcggtc ggcgattcgg caggtgccgg atcgaggcgt gtcccggacg gtgcatcggc
    28081 ccgggtggcc tggtagcgcg cggcatgcat gccgtcgtcg gctggcgact ttcgggattc
    28141 cggtcgggtt tgcagcatgc cggacgggac ccacctcaag ggcgaccgcg cgccgctgtg
    28201 gcatccgcga tcggctatcc tttgagcgag gataggtccg ccatgcttgc tcaaccgtta
    28261 acgcggcagg tggagcacct cggcggtcag gcggtcccgc cagattcgcc aatccgttcg
    28321 atccgcgcgg cagcgaattt gaactccgga atcttgccga acggatcgag ctccggattg
    28381 gtcagaacat tggccggcgc ctcgacgaag cagaagggga tgaagagcat acccctggcg
    28441 acggcgggat cacgtcgcgc catcagctca atcgtgccgc ggcgggtcga gatgcgtacc
    28501 cgctcaccgg gggcaatgcc gagctcgccc aggtcctcgc cgctcatggc gacgaaagcc
    28561 tcgggctcga gcgcatcgag catcgtcgcc cgccgggtca tggcaccggt atgccagtgc
    28621 tcgagcgtgc ggccggtggt caggaccatg ggatagcgcg catccggcgc ctcggcggga
    28681 tcgaggccgc tggccgggac gagcgtggcg cgcccgctgg cggtcgggaa gcggtcggcg
    28741 aagacgacgc cctggccagg gtggtccggg gccgggcagg ggtagccgac cgtgccctcg
    28801 cgctccagcc gcttccaggt gatgttggcg agggacggca tggccagggt catctcggca
    28861 aagacctcct cgggtccgtc gtagcgccag tcgaggccga gccgcctcgc cagctcctgg
    28921 acgatccacc agtcggggcg ggtctcgccc aggaccggca aggccggccg gccgagctgc
    28981 acctgccggt tggtgttggt caccgtgccg gtcttctctg gccaggcggt ggccggcagg
    29041 acgacgtcgg cgaaggaggc ggtctcggtc aggaagatgt cctggacgac gaggtgctcg
    29101 agcttggcga gcccggcccg gacgtggctc gtgtcggggt cggacatggc tgggttctcg
    29161 cccaggacgt acatgccgcg aatagcacca tcgaccgccg cgtccatgat ctcgaccacg
    29221 gtgaggcccg gccgctcggg cagcgtggcg ccccagagcc tctcgagctg ctcgcggtag
    29281 gccgggtcgt cgacgcggtg atagtccggc aggaacatcg ggatcaggcc ggcatccgag
    29341 gcaccctgga cattgttctg gccgcgcaag gggtgcagcc cggtgcccgg ccggccggtg
    29401 tggccgcaga gcagggcgag gctgatcaga cagcgggcat tgtctgtgcc atgggtgtgc
    29461 tggctgacgc ccatgcccca gaagaggatg gcgcgctcgg cgcgggcata gagccgggcg
    29521 acggtgcgga tggtcgaggc gtcgatgccg cagatcggcg ccatggcttc gggcgggtag
    29581 gcggcgagat gctggcggag gtggtcgaac ccctcggtgt gcgcctggag atactgacgg
    29641 tcgaagagct cctcggtgac gatgacgttg agcatggcat tcaaaagcgc cacgtcgcgg
    29701 cccggctcga attgcagcat gtgggtggca tggcggcgga gcgcctggcc gcgcgggtcc
    29761 atgacgacga gcttcgcacc ctgcttcacc gcctgcttga agtaggtggc ggcgaccggg
    29821 tggttctcgg tcgggttggc gccgatgacg acgatcacgt cagcgaggag ggcctcgttg
    29881 aagggtgccg tcaccgcacc cgagccgatg ccctccatca gcgccgccac cgacgaggcg
    29941 tggcagaggc gcgtgcagtg gtcgacattg ttggtgccga agccggtccg caccagcttc
    30001 tggaagagat aggcctcctc gttggagcct ttggccgagc cgaagccggc gagcgcgtcg
    30061 ccgccatggg cgtcccgtat gcgccgaagc ccggcggcgg cggtctcgag cgcctcctcc
    30121 caggtggcct cgcggaaatg gctccagggg ttggcggcgt cgaccagctc gtcggcgcgt
    30181 ttgggtgcgt ccgccttgcg gatcaggggc acggtcagcc gttccgggct gtgcacgtag
    30241 tcgaagccga agcggccctt gacgcagagc cggttctcgt tcgccgggcc gttggccccg
    30301 cttacatagc ggatgcggtc gtccctgacg tggaaggtca ccctgcagcc gacgccgcag
    30361 aaggggcaca cgctctcgac cgagcgatcg ccgatctcgt cctcgtccat gatcgaggct
    30421 ggcatcaggg caccggtcgg gcaggcctgc acgcactcgc cacaggcgac gcaggtggaa
    30481 agacccatgg gatcgtccat gtcgaagacg atcttctcgt ggtggccgcg gccggccatg
    30541 ccgatcacgt cgttgacctg gacctcgcga caggcgcgca cgcagagcgt gcactggatg
    30601 caggcatcga gattgacccg catcgccggg tggctggggt cgggggcagg gcgggcctcg
    30661 gcgggaaagc ggctggtctc gagcccgctt cgttccatcc actgccagag ccggctttcc
    30721 ccgtcgcgcg cccgctcccg cggcggctgg tcggcgagca gcagctccat gaccatgtgg
    30781 cgtgcccggg tcgcccgctc attgccgaca tggaccttca tgccggggct gggcgttctc
    30841 aggcaggagg cggcgagcac gcgctcgccc tcgatctcca ccatgcaggc gcgacaattg
    30901 ccgtcgggcc ggtagccggg ctccggtgcg tagcagagat gcgggatggc gatgccgagc
    30961 cgctcggcca cctgccagat ggtctcgtca ggcgcggcct cgacctcttg gtcatcgaga
    31021 gtgaagcgga tgggatcggt catggcgcct cgttgccgcg gtcggatcgt cgctaaggaa
    31081 accgccgatt cgtgccgagc tgcggcccgc ttgtcaaacc gatgggaatg caggggttca
    31141 gaggcagaag cccggcaggg cgtagtccgg catcgcccca tggcgccgcg cgaggttttc
    31201 caaggccgcc cggtcggcgg ccgggtcggc accttcggga aatgcctcgg catggatgcc
    31261 ccgcatgatg aaggcggcgg cgaggccggc gtccttggcg cccttgatgt cgtgctcgaa
    31321 gctgtcgccg atgccgcaga tcgcctccgc ggcgacgccc ggcagcatat cgcggcaggc
    31381 ggcgtagatg ggggcatggg gtttgcccac atagatcacc cggccaccga tattgtgata
    31441 gcgctcggcg agggcgccgg gtgccatggt caggccgccc tcggtcggtg cgacccggtc
    31501 cgggttcgag cacaccatgg gcaacccgcg cgccgcggcg acggcgatga tccgatcgaa
    31561 ctcggccatg tcgtgggtca gggtgtcgag ccaggtgagg tagacgaact cggcctgccc
    31621 cgggtcctcc accacctcga gatcgagccc ctcgaccgga cccatgtcgc cttcgcgggt
    31681 gaagaggagg cagcgccggc caagcgcctg gaacgcgtcc agggtgcggt tttgcaacgc
    31741 tagccaggtc gcttcgcccg aggtgacgat gccggtcagc acggaggtgt caaagccgag
    31801 ctcgagcagc cgctcggcgt tgtagctgcg ccgccggccg gaattagtca gcaataccaa
    31861 gcgcttgcca cgacggtgca gttcggccaa gagctcgatc gcacccgcat agggtgctgc
    31921 accattgtgc aggacacccc attgatcgat gaggaaggcg tcgaagcgat cgacgaagct
    31981 gccgacgccg tccacgaggg tggttccggc gccgccagcg gcaccggcag gctgggtggt
    32041 tctggccaaa gctgcggtcc tgtgagccat ggggcgggaa ggagaatgcc gtgacaccac
    32101 tcgatccggg cctcgaaggc aagcgcgttt tgatcaccgg cgccagtcgc gggatcggtg
    32161 cggcggcagc ccgcctgctg gctgcctgcg gggcgcgact ggtcctgcac caccgccgcg
    32221 atgccgccga ggcggccacc atcccccatg tcgcgctggt gcagggagac ttcacggagc
    32281 gcgcagcacc ggcccgcacc gtcgaggcgg ccgtggcagc gctgggcggg ctcgacatct
    32341 tgatcaacaa tgcggggcat atgctcggcc ggatcaccct ggacgccatg gacgacgcgg
    32401 cgatcgaccg ggtcttcgac ctgaacgcgc gctcggtggt cgccgccagc cgcgcggcgc
    32461 tgcccgcact ccgtgccagt agcggccaga tcatcaacgt gacgtcgatc tccgcccgca
    32521 ccggcggcag cgccggctcg gcactctatt catcggccaa ggccttcgtg tcgaccttca
    32581 cccgcgcact cgcgaccgag ctcgccgccg acgggattcg cgtcaacgca gtctcgcccg
    32641 gcaccatcgc caccgagttt cacgaggtct actcgagcag cgacaagctc gaggcgaccc
    32701 gccagcggat tccgctgcag cgtcttggcc aaggtgacga ctgcgccggt gccttcctct
    32761 tcctcgcctc ggagcggctc agcggctatg tcagcggcca gatcctcgag gtcaatggcg
    32821 gccagctgat gccctgaccg caagccggca ggagtgttca gagataggtc agggccagca
    32881 cggcgaggcc ggagaaggcg ccgagcccga gcaccatgca ggcgaagagc agcagcgaca
    32941 ccacattgcc gagccggacc aggggatggc gcgagcgctc atgtgcaagg cgaagctggt
    33001 cacggcgctc ccgcccgccg acgatcgcca gatagcgtcg gccgagtggc gtccaaaggc
    33061 tgacccggat gtcgaccgtg tgcccgcccc aggcgagctc ctccgtcgcc tgccgcagtg
    33121 ccaggagctg ctcgtggtcg agcgagcggc gcacctcgag cggcaggcgc aggatgaggg
    33181 atttgtagag accgtggccg tcgatcggtg gggtccagag acccggaccc gtcgccgttg
    33241 cccgagcctc gggcggcacc ggtggtccgg cggggctggt cgatgcgagc attctggagg
    33301 ctctcctgcg atccgcgtag cgggtggcca gggcagggtg acgaccgatt ctgggaacgg
    33361 gtgactcccg gggaccggcg gcagccgcaa tctgcggcaa tgagcctaac aagaggttgc
    33421 ggggcggcgg cggattcccg gccggcgtac agcagtgcga caaagatgca gacagagccc
    33481 gaagctgccc gggtggctag cgaatttgcg aaatattaag gctctgcggc gaggatgcgg
    33541 acatcggggc agcgaccaca gcccgaggga atgtgccgat gtcgatcgag ccgagcgatt
    33601 cgcttcaact gtccgccgcc ctgccgggat ttgcgcgacg atgcggccgg cgcttgcgcg
    33661 agcttctggc cgaagaccga cggggcggtg ttctcctgtt cttcgggctt atcctggtgc
    33721 cgctggtcgg cttcgccggc ctcgccatcg attcgctggt cgccttcagc gtcgaggcca
    33781 gggtgcagag agccctcgac gccgccggtc tcgccgccgg ccgggccgcc cgcgaggagg
    33841 atgttgtcac cgaggcacgg gccttcttcg acgccaatct cggcgccacc ggcgacgaaa
    33901 tcgcaatcag cggtttcgac gcggtctact cgcccgagga cgaatcgatc aatctctcgg
    33961 tccgtgccac ggtgccgacc tacttcatgc gtgtcctcgg caagaccgag accaccatcg
    34021 acctccagac ggtcgtcaac ctgcagatcc ggcagatgga gctggtgctg gtgatggaca
    34081 acaccggctc gatgcgcagc ggcggcaaga tcaacgcgat gaaggacgct gccgccgagc
    34141 tggtgcgcat cgtcttcggc ggccgcgaga gccaccccaa tctctgggtt ggcctggtac
    34201 cctacgtcgc cagcgtcaat atcggtgcgc atcggacagg ttggctggct gctgacgacc
    34261 cggtcttgat cggcgctggt gcctcgccga gcgccggcaa gaagaagggc aagaatgaac
    34321 cggccggccc ggtcgacccc ttcgcaccga gcagctggaa gggctgcgtc atggcccggc
    34381 cggcgccgtt cgacgagacc gacgatccgc cgacggtgga gccgttctcc agctacctct
    34441 acccgagcgg ctccgacaac aactggccac cgctcgacga gagcaatgct gcggagaaca
    34501 acggccgtgg gcccaatctc ggctgcgggc cggccatcac ctcgctgcgc cagagccggg
    34561 cagaggtgct ggcgtcgatc gaggagatgc tgccctggca tcgcggtggc accaccggca
    34621 atctcggcct ggtctggggc tggcgcgtga tctcgccacg ctggcaggga ctctggggcg
    34681 gggccacgcc ggccgagctg ccgctcgcct acaacgacgc cgagagcgac aaggtcgtgg
    34741 tcctgctgac cgatggcgac aaccagttct acgacaaccc gccaagcggt ccgagcggct
    34801 ctgacttcac cgcctatgga cggttgagcg atttcggctt cgcctcgctc gccgcggggc
    34861 gcgacgagct cgaccgtcga atggcccgca cctgcgccgc catgaaggcg gaggggatca
    34921 tgctctacac gatcaccttc ggcccgaccc cgaacagcgg cacccagaca ctcttccgca
    34981 cctgcgccac cagtcccggc cactacttcc attcgccgaa caacagcgac ctctcgagcg
    35041 tcttccgggc catcgggcgg cagttgtcga gcctcagggt ggcacgatga gcgacgggcg
    35101 gcgtcagcgc tttggcgctg cccgggcgtt cggtccggat cggaccggca acaccatggt
    35161 cgagttcgcc ctgatcgggc cggtcttcct ggtcatggtg ctcggcatct tcgagatctg
    35221 cctcattctc ttcaccagcg gctcgctgca gtcggcggtg gcctccgcct cccgctacgg
    35281 catcaccgga caggccggcg ataccgaccg cctgacggcg atcaccgaga tcctccgcga
    35341 ccggaccttc ggcttcatcg atatcgagga agccgacatc tccacgctgg tctatccgag
    35401 cttcaccgaa atcggccgcc cggagccgtt caccgaccag gacggcgatg ccaccttcga
    35461 tccgggcgag ccctacgagg acatcaacgg gagcggcggc tgggatgccg acatgggctc
    35521 ggccggcctc ggcgggccag gcgacattgt gctctacaac gtcgactacc gcagccggct
    35581 gatgaacgcc ttcgtcgcac cgcttttcgg cgacatccgc tatacggcga ccatcgccgt
    35641 gcgcaacgag ccctattgag gcatgtgtgg acccatgctc cccggggtgg cgcacgctgc
    35701 ccgtccgcgc atctacgcat tcgcccgagc agcacacatc ggggcatggc gcatgtgggt
    35761 tgccggcctt cgggggcgtc tgtcgggcga cctgtgtcgc gggcacctcg cggctgatcg
    35821 ccggggcgtc attgcgctcg agttcgcgct cattctgccg ctcctgatcc tcttctgctt
    35881 cggcacggtc gaggtcgggc gcttcgtgct gctgcagatg aagctcgacc agctcgccgc
    35941 ctcggtggcc gatctcggca cccgtgaccg cacgctgagc gccgctacga tcgacgacat
    36001 tttcgccgcc ggggcgcata tcgtccggcc cttcgatctc gccgccgacg gccgcatcac
    36061 actgagcgcg gtcggcctga cggccggcga tcagccgcgc gtgctctggc agcggcgcag
    36121 cggcggcacg ctcgacctgc cgagcgagac tggcacggtc ggcagccccg ccacgctgcc
    36181 cgacgagatc acgctcgctg tcggccagac cgtggtcgtc gccgaggtca gcttcagcta
    36241 tcgtggcatg ttcggcctgc tgcccgagcg gatcacgacg aagcgggcct atttccgccc
    36301 gcgcctcggc agcctgcagg agctgtcgtc gattggcggt tccgatcatc ccttgattgc
    36361 agacgccggt tggtgcgagt cgttgcaggc gtcagttgtt gcaagcggat gacgctgggc
    36421 gcctgccggg cacgagtcgg gtgatggtcg cttgagccga gagccacgac cccttagatc
    36481 ccctgcagtc ggctgactgc agccggacgt tcgagcgtca tcgacttcta tgtcccgtgc
    36541 ccggcgggac gggaaggttg ttgtcgccgg atggcgttct ctttcggcgg ttaaggcggg
    36601 tggtcccgca tggcagccgg ggagcagcat tcaggtgttc gatgacgccg atttccgcac
    36661 tggtccgccc agccctctcc agcctggaga agaagctgac gcgcgccgtg atcctctatc
    36721 ccacggcgcc tttcatggcc gccgaccggc gcgtggcact ggagcgctgg ctgcgcggcc
    36781 gtgagcagtg ccagaagctc ggcaaggccg acatcgtcat cgtctccttc ggcaagagcg
    36841 gcaggacttg gttgcgcgtc atgttgtcgc gcttttatca gctgcggcac gccctgtcgg
    36901 aacggttcct catcggcttc gacaactacc atttcatgaa ccgggcggtg ccgcggatct
    36961 tcttcaccca tgacaactac gtgaaggact acagcggcaa cgtcgacaac aaggccgact
    37021 attacggcaa gcgggtggtt ctcctggtac gccatccggc cgacgtcgcc gtgtcgcagt
    37081 atcaccaatg gcgctttcgg atgcggccac gcaagaaggt gatcaacgac tatccggacg
    37141 aggcgggtga ggtgtcggtc ttcgactttg tccgtcacca gccggctggc ctggagaaga
    37201 ccatcgactt tctcaatggc tgggcccgcg agcgcgagcg gctcggtgct tgccacatcc
    37261 tgcgctacga ggacatgcgc gccgacccgg ccggcagcct cgggcagttg ctcgacttcg
    37321 tgggcacacc gggcacgccc gcggagatcc ttggagcggt cgactatgcc tcgttcgaga
    37381 atatgaagaa gctcgagggg cagagccggt tctggctttc cggcgggcgg atgatggcca
    37441 aggacaagag caatcccgag agcttcaagg tgcggcgcgg caaggtcggc gggttccgcg
    37501 actacttcac cgacgccgag gtggactcga tcctcgatta tgtggatgaa cgcctcgatc
    37561 cggtgttcgg ctatcgccgg acggtcatgg ccccgtccgg cagcgaggtc taacgctgtt
    37621 ggaacggctg gtaggacgga gaacaggatg agcgaaccca ggccttgcgt gttcattcac
    37681 accaacccga agcaaatgct cggcgcccgg gtgtcggaat attcgtttcg acgcaacagc
    37741 gccagacccg acgcgttcga cgtgcgcatc atctcgactg aggatttccc ctggctgaag
    37801 agccacgagg gccggaccta ccggcgcgac ggtctcgacc gcgaatggct gtatgacgat
    37861 ctgcagtcct tcacacctct gcgcttcacc ccaccagagc tcatgaacta ttccggccgg
    37921 gccgtgatcg tcgatcccga tgtctttgcg gtcggcgaca ttctcgacct cctgaagcgc
    37981 gatatggcgg ggaagccgct cttcgcccgg ccgcgcggcg gttcgaagga gttcgcctcg
    38041 agcgtcatgc tgctcgactg cgccagactc acccactggc gggccgagga gcaattcgac
    38101 gagctcttcc agggcaagcg tgactacatg gattggatct gcctcaagct cgagccgcgc
    38161 gagaccatcg gcctgctcga gcccgaatgg aacgacttcg accggctgac gccgtcgacg
    38221 aagctgctgc acaacaccaa acgctggacc cagccctgga agaccggtct cccggtcgac
    38281 ttcacaccgt cgagcaagac ccgacgcttc gccccgctcg gttggtttcg tcgggcacgc
    38341 gaggcagtgc tcggtcgata tggcggtctc ggaacttaca agcggcatcc ggacccgggc
    38401 caggaggcct tcttcttcgg tctcctcaag gagtgcctcg acaaggggca tatcgacgag
    38461 gccatggtgc gggccgagat ggccgccaat catgtcaggc acgacgccct ggagatgctc
    38521 gagcgatcga ccctccagca ggcggcctga gccggtcggc cgcgtcggga tgtcgtgggc
    38581 cgagcgcagg cgctactggc gcaagcgctg gcgccggctc gcggtggtgc ggtccttgcg
    38641 acttgggcta cgcgacgcct tcctcctcag cttctgggcg atctgcgcgc ggctctcacc
    38701 aactcgggca tcgcgcttgg gcgccgcctg cagccgggtg ctcggtcggt tgagcaccaa
    38761 gaacgacaag attcgccaga acttgcgtat cgtgtttccc caggccggcc gcggcgagct
    38821 cgaccggatc gagcaggcca tctggtcgaa catgggggcg gtcatcggcg agtatccgca
    38881 tctcggggtg ctcggtcggt cggcgggtga tggggtcggt ctggaatcgg cgatcccgcc
    38941 gcatctggag cccaacttca gcggtgagcg gctgactgtc ttcgtcgcgg gccatctcgc
    39001 caactgggag gtgctggcgg tggcaccggc gcactggggt gtcgatctcg ccgtcatcta
    39061 cacacccatc ggcgagggtg ccgccgaccg gcgcctgcgt gaataccgcg agcgcatgtc
    39121 ggctcgcctc ctgccgcgcg acggcagtgc ccgcgagctg ctgcggcatc tcaggagcgg
    39181 ccggccggtt ggcatcgttg ccgatcaccg tgccgacgag ggggcgtccc tgccgttctt
    39241 cggcgccaca aagcgcacca cgctctcgcc ggcgcggctc gccctgcgga ccggtgccga
    39301 gctcgttgcc gttcgggtgg agcgcctggg gccggcccac ttccgcctct ccgccgaagg
    39361 gccgatccgc ccgccggcgg gcgtcggcga cgaggagacc aaggcggtgg ccatgatgac
    39421 cgcgttcaat gcatgcctcg agcgctggat ccgggagcgg cccggcgagt ggctctgcag
    39481 caagcgggcc tttcccaagg aggtcgtcga ttctctgccg tcgcggccgc tcggcggctc
    39541 gctcaagccg gacatctcgc agcatgctta gcgacaacgt caagaaggcc ctcgtcctcg
    39601 ccgccagcca gcccttgccg ccggcctggc gcctgcgcct gcggcgcccg gggctcgccc
    39661 ggctggagct ggagaaggcg acccggaccg acctcttgat catcggtcat cccaagagcg
    39721 gcaacacgtg gcttcgggtc ctgctctccc ggctctacca gacacggcac aacttgccgg
    39781 ccgacctggt cgtcacctcc gacgagctgc atcggcggaa cgcggccatc ccgcgcatct
    39841 ccgcgaccaa tggctggtat tcctatgagg gggtggtcgg ggacgccctg gcggtcgacg
    39901 caccgccgag cccgattcgc gataagccca tcgtcttcct gccgcgccat ccgctcgaca
    39961 tcgcgacgtc ctggtacctg caggtcacca agcgccagtc gaacgcgaag ttcgagctga
    40021 tcaacgccga tctcgaccgg ccgatcgacc ggactcggat cgatcgctgg gccttcgtcc
    40081 gccacagcgg attcggtctc gccaacatca tcgcctttct caacacctgg caggcgcgag
    40141 tcgcccggct gccgcatgcg atcaccgtac cctacgaggc gttgcgcgcc gatccggcga
    40201 gccagctggc acggatcggc gagctgatgg gcgacccgtt cagcgcggac gagatcgccg
    40261 aggcggtgcg tttcggcagc ttcgacaacc tgaagtcgct cgagagcagc gggttcttca
    40321 agcagaaggg gctgcgccca ggcattcgag gcgatgccga gagcagcaag gtgcgtcgcg
    40381 ccagagtcgg tggatatcgc gaggacttca cgcccgagca ggtggccgag ctcgaggcgc
    40441 tggtcgcggc atcgctcgat ccggcgctgg gctatgcgcc ccgaccggca tcggccggcg
    40501 ccgaaccttg agcgacgcgc ccgtcacctg gctgctcctc ggcgacaagc gcggcgacaa
    40561 ccgcatggtc gaggtgatcg ccgagcatct gccctggcct tcgatcacca agcggctggt
    40621 cacgcggccg gaatgggtag tgccgaagcc gcgggttcgc tcgaccctgg accacctcga
    40681 tctcgccgcc tccgatccgc tggagccgcc ctggcccgat ctggtcctga ccatcggccg
    40741 gcggccgtcc atggcggcaa gatggctggc ggaacaggcg gaaggccgga cgaagctggt
    40801 cctggtcggc aagccctccg gctcgatcga accctacgac ctcgttatcg tcagcgccga
    40861 ggcgttcctg ccaccgtcca cccgggtgat gaccatcgat ctgccgctga tgcgcatcga
    40921 tccgaccttg gtcgctgccg aggccgagct ctggcggccg cggctcgccg ccttgccgcg
    40981 gccctggatc gcctttcttc tcggtgggcc gacgggtcca ttccgctacg acgatgagct
    41041 ggtcgagcgg gtgctggatg gtgcccgcag gaccgcctcg ggcgagggtg gcacggcctt
    41101 cattgtcacc agccgtcgca cgccgaaacc cgtggtcgag cgcctgcgcc aggagctgct
    41161 gcccgccgcc cggctatatg cctggggcga cgaggccgcc ggcaatccct atctggcgct
    41221 gctcggcctc gccgatgcgc tcctcgtcac cgccgacagc atctccatgc tgaccgaggc
    41281 agcgcgactc ggccggcccc tttccgtcat cccggtctcg accccggggc tcggccggct
    41341 cgaccagtgg cgccgcgagc tcgtgcagcg cctgttcggc ggccgcgccg ctcgctacgc
    41401 cccgctgctc ggccggctcg gcatctcggc gcagacccgc gatttcgctg ctttcgaacg
    41461 gctcctggtg caacgcggcc ttgcaagctc ttacaccatc cgaccgccgg ccccgaccgg
    41521 ccgggccggc gacgatgtcg gccgggtggt ggcgcggctc tgccagctca tggggcatgc
    41581 gctcccaaag ccacagagcg cggccaattc ggtcgatgcg acaccggtcg ccgccgaccg
    41641 cgttgtcggt gtccaaagcc gatgataatt tttgtcgcgg ggcatgtgga catgccccgc
    41701 gacggtgcag ccgaatggcg ttgtgcccgg cctaaatcgg ggagaatccg gcatggccca
    41761 ggccattgct cgagcgacca attcattgtc gccggtccca ccgcccttcg attatgcgcg
    41821 gctcgcagcc acggagcgcc gggatgatcc gttcccgcat gttctcgtcg agaatttcgt
    41881 caccgtgccg gaccgggcgg cgctgatcag cgattttccg gcgatcacgg ggcctggcaa
    41941 tgtggagccg ggcgacgtgc cgcaggggcc ggccttcgac cggttgatcc tagagttgga
    42001 aagcgaccgc ttccgtgccg caattgccga caagttcggc atcgatctct ccaacgccgt
    42061 gccgaccatc ggcatccgcg cgtttgccga gccgaccgat gggcgcatcc actgcgacca
    42121 ccggtcgaag ctgatcacgc tcctgcttta tttcaacgag gaatggacag acgaaggcgg
    42181 gcggttgcgc atctgccgcg accaaagcga catcgaggat ttcgccacgg aggtggtgcc
    42241 gctctccggc acgctcattg ccttcaggaa cggcccgacc gcctggcatg gccacaagca
    42301 gcacgttggc cagcggcgca tgctgcagct cagctttcgc gacatgagcg gcattgtcgg
    42361 gatcgagcgc cggctgtcgc ggctgaccaa gccgatccgg cggatgctca acctgagctg
    42421 atggcagcac cggtatccga gctgtcgccg attgtgtttg tcttgtagcc gaagagttgg
    42481 ccgggccggg cctggaccat tgcctcgacc gcatcgcatt cggcggcact caaatagtcg
    42541 cgccagccgc cgaccttgcc gcgccgcacc ttgaagcttt cgggattggc gacgtcgccg
    42601 ggcttcagac gctgcccctt gtagccggca tccgcgccag ccgcctcgag tcgccgcatg
    42661 ttctcgacag aggcaaaggc aacggcgccc tcgaccgccc ccattggcgc cggtgtgccg
    42721 agaaaatcga ggatggagga gagtacggct gccgtctggc cgcgcagcgc ctcgtagcgg
    42781 acgacctgca cggaatcgag ctgcggcagc tcacgctccc agagctcgag gaagtcgagc
    42841 acgccggcga gtcctgccgg ctcctgcagc acgaactccg caacgctgtg cccggacccg
    42901 tgcggcgggt aggcattgag cgccttcttc gccgggtgca tgcggtgctt ccactggaag
    42961 tattgcgaga ccgcggtgtc gcgcgggtcg cgcaccagga gcagcacgcg ctttcgccgg
    43021 aacgccgcgc gatcggtcca ctgatcgaga taatcgcgaa ggtagttgcc gtgagtgaag
    43081 agcacgctcg ggatccgggg gtccagccgt ttcaggttgt cgaactcgag cgccgtgccc
    43141 tcgggaatgt cataggcgag ctggtaggcg cgcgagagca tggtcctgag ccaggtccgg
    43201 ccgctcttgg cgtagctgac gaaggcgacg tcggcccggg ccagaagccg cgtctcctcc
    43261 cagcccctga gccagcgctc gatgccgatg cgccgcgccc gcggcaggaa gaacaatccc
    43321 tggcgcaggc cgtagccgag gccgacgacg agctcgtgtc gcatggtggg ctgtccgctc
    43381 ttccgctgct cgtcgggctc gcccgacctc gtttgatggt gactcgaggg ggtccggctg
    43441 atgtacgcaa agccggcccg ctgctcaagc cgtgcccggt tcagtcgcta tccttcgagg
    43501 tgtcgccggc cgccgcccgg ccagccgcct cgcgcacacg tgattggtcg tcgtcgagcc
    43561 ggagcgccaa ctcggcccgt gccagcgcaa gcgtcgcgaa gtgggggacg gcacgggtca
    43621 gggccgcgca tgacggccgg ccgagagccg aaagcgcctt ctgcaacctg atctgcacct
    43681 cgaccagccc tgcaccgtcc cgggcaatcg cgttgaatgc atcatcaagc agatcgctcc
    43741 aagcgagctc tggcacgtgc acgcggtcgc acgcgacctc gtccgccgcc ggctcggcgt
    43801 attcggccgc gtgggtcgcg aggatgcgga cctgtgcgcc gatgatcgcg atcgccgtcc
    43861 ccggatcgtt gatgcccggc gacagggcgc gcccggcgat ctcgctgagc accacgagcc
    43921 cgaagcgcgg gtcctggtcg aagctccgct cggcgccgac cagaaaggcc gcccggagct
    43981 cgtcctcgtc gatctcgtcg tcaggcacct cgccccgcat cgaccagtcg cgggcgacat
    44041 ggacgagcgg ctgcccgggg gcgacgaact tgcccggcaa ggccgccagc gtgaggcgca
    44101 ggtcggctgc ctcggcggct ctttgcaagc ggtcgatgtc gatatactgg acgtaaccga
    44161 tcgagtcgcc ggcgaccggg cggccgggat cctgaccgcc ctcgaccggc cgcccgccga
    44221 gccggggccg ccgacgccga ttggcgaagg cccgcagagc cgccgattcc acccgggcga
    44281 tggtctcgcc catgcgcccg agccgggcga tcgtatccag ccagtagacg aaggtgagga
    44341 ccacccccgc gaagacgccg agcgtcatca ggaagaggac gaaattgcca gcctgaccat
    44401 agatcccgac cctgaccgcg accagcccga ccaccccgaa gatgaaggcg ccgatgaagg
    44461 tcgagagtgc gcgcttcgag cggtcgtcgg cgaccaccag gcgaaaggcg cgcggcgtcg
    44521 ctgcctggct cgccgagttg taggcgccga ccatggagga gaccgcgaag gtggcgaccg
    44581 ccagcatggt cgtggcgatc acctgcaata ggatctgcag agtctccggg tcgatcttcg
    44641 gtacccagtc gccaagcccc gtgccatcgg cgaagcgggc gaggaaggtg acgctcaccg
    44701 cgccgatgca gtagatcgac ggcatgagcc agagccgttc gcccagccgg ttgagatgga
    44761 accggatgcg ggagaaattc atgccggtcc ccttgcccgg tcgaccacgt cgccgaccac
    44821 gagcagcgcc gggccgtcga gcggctcggc ccccctaacc agctccggca gggtggcgag
    44881 ggtgccggtg atccggcggg cggtcggcag cgtgccgttc aggatcaggc aggcggcgag
    44941 gtcggaggcc agaccgtgcc cgatcagccg ggcggtcagc cggtccagca ccgcatgccc
    45001 catgtagaag accagcgtct ggcgtggccg gcagagcgcc tgccagtcga gcgccggctc
    45061 gccgtcctgg ctgtgaccac tgaccaggat cagcgtctgc gccaggtcgc gatgggtgag
    45121 ggggatcagg ctctgggcgg cgcagcctgc ggcggcggtg actccgggca ccacctcgac
    45181 cgggatgccg gccagagcca ggctttcgat ctcctcgccg ccccgaccga agatcagggg
    45241 atcgccgccc tttaggcgga ccaccagcag cccggcgcgg gcacggtcga tcatcagggc
    45301 gtgaatctcc gcctggcgca tgtggtgctc cgcacgtcgc ttgccgacga agatcagctc
    45361 cgccccgggc cggcagtgct cgagcacgcc ggctcccacg aggcgatcgt gcagcaccac
    45421 gtcggcgcgc ccgagcagcc gcgccgcctt gagcgtcagg agctccggat cgccgggccc
    45481 ggcgcccacc agatagacgc ggccgctgac ggtggtggct gtcgacacgg cgttccttcg
    45541 tgaaggttgt gccttcagta gcccgctcag cgccaggcga cgagggcaaa gaggaaggtc
    45601 agctcactga gctgctggac ggcccccagc gtgtcaccgg tgcagccgcc gaaggcacgt
    45661 ccgagcgcct tggcggtgaa cgccgccacc agggcggcgg ccagcatggc gcccaggccc
    45721 tggccggtcg gcaggatcag gatggtcagg gtggcgccaa tggcgaggcc aatgagcacc
    45781 cggtcggtgg gcggccggcc ggccgtggcg gccaggccct ggctccgggc actgggctgg
    45841 gcccacatca ccagcggcat gagggcgcgc gaaaaggctg ccgccgcgac cagtgccgcg
    45901 atggcgcgca agggctccca gaatccggcg agggcaccga gcctgccgcc cacgacgagg
    45961 atcagggcga gcgtgccgaa gctgccgatc cggctgtccc gcatcaccgc gagcgcccgc
    46021 gcctggtccg gcagggcgcc cagggcatcg gcggtgtcgg cgagcccgtc ctcgtgcaag
    46081 gcaccggtca gcagcagcat ggcgcccagg gccaggaccg cagccggcca cgagggcaga
    46141 tggagaaaat gagcgagggt aaaggcggcg ccgcccgcga ctccgaccag cacgcccacg
    46201 accgggaacc agtgcaccac cgaggcgagg tcgcccatgc ctaccggccc atttggccgg
    46261 agcggcaggc gggtcagaaa gacgagggcg attgcgaggt cccgcagcat cattccagtc
    46321 ggatcgtggc gctacggcac tgggctatct cgacaaatgg ctcgcgtcaa agcaagttgc
    46381 cggccaatcg aaggatgcag ccttgagcag cctgacccca ctggccaact tcgccgacct
    46441 ccgctacctg ctgcgtcagc tgccggcgat cgacggcaac gctgtggcgg cggcccgtgc
    46501 ccgggaggcg gttctgctca agccggcggg ggcgcttgga cgcctcgagc agatcgcgag
    46561 ctggctcgcg ggctggcagg gtcggcaccc accccgcatc gagcacaccg ccatcttcgt
    46621 cttcgccggc aatcacggcg tcgccgacca gggagtctcg gcctatccgg caaccgtcac
    46681 ggccgcgatg gtggaaagct tcgaggcggg cggcgccgcg atcaatcagc tcgcgcgcat
    46741 cgccggcgcc gagctgcatg tcgtaccgct ccgcctcgac cgaccgaccc atgacttcac
    46801 gctacagccg gcgatggacg aagcggcgtg cgttgccgcg ctcaacaccg gcctgacggc
    46861 gcccgacgac gcactcgacg tgctcgccgt gggcgagatg ggcatcggca acacgacggt
    46921 agccgccgcg ctcgccacgg ccctcttcgc cgaagctggc gagcatcccg acccggccca
    46981 gtgggtcgga gccggcaccg gcgtggacgc gaccggcatg gcgcaaaagt cggcggtgat
    47041 cagtcgggcg ctggcgcggc acgaggccgt tcttggcgac ccgttggagg tcctgcgtca
    47101 tttcggtggg ttcgagctcg ccgccatcct cggtgccatt gtcgccggcc ggctgcgccg
    47161 cacgccggtc gttctcgacg gcttcgtcgc caccgccgcc gccgccgtcc tgcagcggct
    47221 gcgaccggat gcgctcgatc attgcctcgc tggccacgtc tcggccgaac gggggcaccg
    47281 aagactcctc gcggaactcg gcctgcgccc gttgctcgat ctcgatctca ggctcggcga
    47341 gggcaccggt gcggccctgg cgctcggcct gttgcgcgcc gccgcggcct gccatagcgg
    47401 catggcgacc tttgccgaag ccggtgtcgc tgcgtcgccc aacaaggggg cgtagctgcc
    47461 atgacccgcc accgtacggc gtcggcgcgg agcaccggcc cccggagctg gcccattctg
    47521 ctgctgtttc tgtcgtttct ggcgttcggc atcccgggtg ccgcccaggg tacgccggcc
    47581 ccagatcaga cagggggggc gcctcctccg gcaaccggag cggccgatcg tggcggaccg
    47641 ccgtcggccc gcacgaatgc cgtcacaatt gccgatccgg cggaactcgt cgtctggaac
    47701 cgcccgatcg tcaccttgcg ggcgaagatc gacaacgtca cccccgccga gcgggtcggg
    47761 cgcgcttcgc aggagctggc ggctctccgg caggaagagc cgaccggcga ggcggtcgct
    47821 gttccggccc agctcggcgc gcttcagggc tacttcatcg agatcgacaa ccgtcagatt
    47881 ttcggcctgc tgccccagga tgtcgacccg ttgcagcgcg agaccctgga gcaggcggcg
    47941 atcgccgcgg ccgggcgact gaatgccgcc cttcgggttc gcgccgagca gcgcagcctg
    48001 ccgatgatcc tgcgcagctt cggcctcgcc agcctcgcca ccgccgtgct cgttctcatg
    48061 atcctgggca tcgtctggtt gcgccgactg acgctccggc agctcacggc actgagccgc
    48121 ggcaatgcta tcaccgtcgc cggcatcgat cttcggccct acagccgggc gacgggcaca
    48181 gcagtcgtca aagcgctagg cgtcgccgcc cagctggtgt gcgtctatct gtggttggca
    48241 ttcgtgctgc tgcagttctc ctacacccgc ccctggggcg aagggctcgg catctggctc
    48301 atcagcctcc tcggcgagtt cagccacgcg atcctgagcg cggtccccgg cctcttcact
    48361 gtcgccatca tcttcatcat gacccggttc gttgccgatc tggtgaaggg cttcttcaag
    48421 cgggtcgaag ccggctggct tcgggtccac tggctcgagg ccgaaaccgc gcgcgccacc
    48481 cgccgcctgg tcatcgtgct gctgtggctc ttcgcgctga ccgtcgccta tccctatatc
    48541 ccgggctcgc agacggacgc
//
LOCUS       DAOTFI010000003        43003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_916_length_43003_cov_7.073996, whole genome shotgun sequence.
ACCESSION   DAOTFI010000003 DAOTFI010000000
VERSION     DAOTFI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 43003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43003
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_43003_cov_7.073996"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="P5558_00430"
     CDS             complement(<1..1753)
                     /locus_tag="P5558_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY22804.1"
                     /translation="MSTTASDLPQPFAGWFASRGWRPRAHQLAMLVEARAGRDTLLIA
                     PTGGGKTLAGFLPSLVDLAERPTTGLHTLYVSPLKALTTDIARNLEAPLAEMGLGVTY
                     AVRTGDTPANRRQRQRTHPPQILLTTPESLALLLSYENAATYFQNLACVILDELHSIA
                     ESKRGALLSLGMARLRSLAPEARFVGLSATVADPPALLRFLAPDPTRARIVQAEQGAA
                     PEIVIADAEAAMPWSGHAALYSAEAVYARLLAVRTGLVFVNTRALAELFFAALWRINS
                     ENLPIALHHGSLAVEQRRKVEAAMARGALRGVVCTSSLDLGIDWGDVDLVLQIGAPKG
                     ASRLLQRIGRANHRLDEASRAVLVPANRFEVLECIAAIEAIEAGELDGAPPLGPALDV
                     LAQHVTGVACSGPFDAEALFDEVRRAQPYAELPRADFDAVLDFVATGGYALRQYERYR
                     RLEQGPDGLWQLTDRRLVRGYRMNIGTIVEAPVMRVRLGGGRGRGRSLGEIEEYFVSM
                     LTPGDSFLFAGRVLAFEGIRQNEVIASLAKGAREPKIPAYAGGRLPLTSQLAHRVRRL
                     LAEPAHWPALPAPVREWL"
     gene            1814..3400
                     /locus_tag="P5558_00435"
     CDS             1814..3400
                     /locus_tag="P5558_00435"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRY22805.1"
                     /translation="MDEETLRASKAWPITEALALLQRVERKPPAKGFVLFETGYGPSG
                     LPHIGTFAEVFRTTLIRQAFHRLSDVPTKLYAFSDDMDGLRKLPDNIPHPEVILPFLQ
                     KPLTAIPDPFGTHESYGLNMNARLRAFLDRFGFDYEFKSATEEYRKGTFNEVLLRVLE
                     RHEAVADVVRPHLGPERRASYSPILPISPKSGRVLQVAIEECRPATGTVVFRDEDGTL
                     TEVPVTDGRCKLQWRADWALRWTALEVDYEMSGKDLIDSVKLGSQICRVMGGTPPLGF
                     TYELFLDENGEKISKSRGNGITIDEWLRYATPESLMLFLYRDPKKAKKLYFDAIPRNV
                     DDYESFVAAFEGQVDKEKLGNPVWHIHDGKPPEGAVPINYSMLLNLASVVNAEDPEVL
                     WGFIRQYSPKLTPESEPRLARLVDHAIAYYQDRVRPEKRHREPDATERAAMLELEAAL
                     AGLAEGTPGEAIQNEVYEIGKRHGFESLRDWFKALYEVLLGQEQGPRFGSFVALYGVA
                     NTRRLIREALGLPAAAVPGE"
     gene            3393..4664
                     /locus_tag="P5558_00440"
     CDS             3393..4664
                     /locus_tag="P5558_00440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRY22806.1"
                     /translation="MSEFSIGLEARTLALAVLDAALNERAPAEARFAADPATERLEPR
                     DRAFARLLYTTVLRQRAVLDAALLPLLRHPPPHKSGMNLLRLGAAQLLLLGTPPHAAV
                     GLTVELAKKRRIVPPALVNAVLRRLVGTKPPVVDPLANLPDWLRASWSTAYGAETAAA
                     IATALAKEPPLDLSVKTDAAGWAARLGGEVVGPATVRLREAGAVERLPGYRAGAWWVQ
                     DLAASLPARLVGAKPGMAVLDLCAAPGGKTAQLVQAGAKVTAVERERGRAERLRENLA
                     RLDLEAEIVVADAMDYAPATPFDAVLLDAPCSATGTIRRHPDILWSKTEADVQRLAGL
                     QGRLLDAAARLVRPGGTLVHAVCSLEPEEGPAVVEAFLSRHPGFVRQPIAEAECGFAP
                     TPSGDLRTHPALLAEKGGMDGFFIARFTKSR"
     gene            4673..6220
                     /gene="purH"
                     /locus_tag="P5558_00445"
     CDS             4673..6220
                     /gene="purH"
                     /locus_tag="P5558_00445"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRY22807.1"
                     /translation="MSKSLPRRALLSVFDKTGLVELATALAGHGVELVSTGGTARALR
                     EAGLEVVDVSAISGRPEILDGRVKTLQPEIHGGILARRADPAHMAVLAEHGIATIDLV
                     VVNLYPFARTLASGADFATCIENIDIGGPAMIRAAAKNHEDVAILTDPADYPRLVEAL
                     AAGGTDGALRQELARKAFAHTAAYDAAIATWLDGLDGSPLPDPLLVAAPLQQTLRYGE
                     NPHQAGALHAWPQGRPGVALARQLQGKELSYNNLQDADAAVELVAGFTRPTIVIVKHT
                     NPCGVASAATLHEAWERALAADPVSAFGGIVASNRPVDAATAEGLAGLFLEVVVAPGF
                     APEALERLAAKANLRLLVLDRLPGPGEAMLDVRSIGGGLLVQARDTTTSDPASWETVT
                     RRAPTAEERAALAFAWTVCRQVRSNAIVVAAGETTLGIGGGQTSRVEAVELACRGLRV
                     EPAAGPLVLASDAFFPFADGIERAAGAGIRAVVQPGGSLRDGEVIAACDAHDIAMVTT
                     GRRHFKH"
     gene            6493..7191
                     /locus_tag="P5558_00450"
     CDS             6493..7191
                     /locus_tag="P5558_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY22808.1"
                     /translation="MAVQEEAGMAEAQILVVDVGGNNIKIKHAAHVEKRKTKSGPEMT
                     PERMVDAVRKLAGDWRFDHVTIGYPGPVREGRLVLEPHNLGRGWTDFDFAAAFDRPVK
                     VVNDAVMQAIGSYAGGKMLFLGLGTGLGAALVGDHVAVPLEVAHLPYRKKFTFEDCVG
                     KRGLDRMKQKRWEKAVHDVVERLRRALVADYVVLGGGNTKHLQAMPQHSQPGDNENAF
                     LGGVRIWQEHFRVL"
     gene            7214..8599
                     /locus_tag="P5558_00455"
     CDS             7214..8599
                     /locus_tag="P5558_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRY22809.1"
                     /translation="MPGRPGGPGRNARLTSDPLPGLLRAIAIPAGTGLLFQTLFNIVD
                     SFWAGFVSTAALTALGASFPVFFLVIVLSVGIGMAATALIANCLGREDQEGGRRLFGQ
                     AVFLTLVGGVIVALYGLFAIRPLLRFLGTSGESLDAGVDYLLPIMLLSVVFLLNQTLN
                     GWLTAQGDSRSFRNALALGCALNVVLDPLLMFGAFGVPGLGVLGIAIATVLIQAMQLV
                     YLVHRARRSPMGLRLRLADIRPDPKVLRDFLRQAVPVTVSIGATGFGLFCITWFMGRH
                     GEAAIAAYGIGLRIEQLVMLPMIGLNTAALTLVGQNHGAGRPDRVRETARLVMLHGAA
                     VMALGAVVVWLTRAHLIGIFSDDPDVVRLGAAYLGFAVFNFNAYAILILGGGILQGMR
                     LPLYAMGVALFRHVFGPLVVLYLLDPVMGFGLPGIYWGIVIIAWIGSAVTLFYLRREL
                     PDLPAAESLTR"
     gene            8743..9150
                     /locus_tag="P5558_00460"
     CDS             8743..9150
                     /locus_tag="P5558_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY22810.1"
                     /translation="MFGKFTFASKADYATLDWGRVGALCSPSGTGAKQLTILEGRLYP
                     NKGHDFHKHVNQEEVIFVVSGQIEQWIDNEKQILGPGDSAFIPPDTVHASFNAGDGEA
                     NILAIFGPSLGENGFEMVDMATEAPWNSLRPKS"
     gene            complement(9233..9460)
                     /locus_tag="P5558_00465"
     CDS             complement(9233..9460)
                     /locus_tag="P5558_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HRY22811.1"
                     /translation="MADEEVYLVDGEEVVLTDRMHVQCDGGNGALGHPIEYLTLEKGG
                     QTVCKYCDRRYVHKSRAEAEAIRRAGQRFAA"
     gene            9512..10546
                     /locus_tag="P5558_00470"
     CDS             9512..10546
                     /locus_tag="P5558_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY22812.1"
                     /translation="MNARAAFESSTTGAAAPLPAYAIELKGLRKVYRASGGQPDKEAL
                     KGIDLQVPRGAIFGLLGPNGAGKSTLINILAGLVVKTGGSAEVFGIDIDRHPRRARLA
                     IGVVPQELNLDAFFSPREVLELQAGLYGVPKRARTTDAILAAVGLADKADAYARSLSG
                     GMRRRLLVAKALVHHPPVVILDEPTAGVDVELRRQLWAHMRELNARGITIVLTTHYLE
                     EAEAMCDSIAIIDQGRVVACDTTATLLRQLDEKMLTLTLDQPLARLPERLAALGAELD
                     EAGRIVVRYRPSRSRVGEILEAASAAGLAISDLATAEADLEELFLRLTGPAAARDEHH
                     DALPRARSAL"
     gene            10558..10634
                     /locus_tag="P5558_00475"
     tRNA            10558..10634
                     /locus_tag="P5558_00475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10592..10594,aa:Arg,seq:ccg)
     gene            complement(10687..11100)
                     /locus_tag="P5558_00480"
     CDS             complement(10687..11100)
                     /locus_tag="P5558_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY22813.1"
                     /translation="MHQKSDLGTMIAALDVGVTRSAQDQGLARLTPLHREVVRGMPRR
                     QDQEIRVLSATLLPGDVTPYHSHRHPVTVYMLEGTFTLELDDRGTVSIEAGEIFVEPS
                     GVNMTGSNQGNVPARMTLFYVCEPDEPFADPASRA"
     gene            11194..12096
                     /locus_tag="P5558_00485"
     CDS             11194..12096
                     /locus_tag="P5558_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY22814.1"
                     /translation="MRAMFDWNDLRHLIAVSRAGSTLAAARALGVNQSTVHRRLVELE
                     RRIGLGLVKRRPSGYRLSELGEALIDDLLAVETAVGDLERKIRSLKLDLAGTIRLTCP
                     EPIVPRIAASGLLDRFHERYPGLLVELVTSDRYLNLAEGEADIAFRSGEPVDDRLVGR
                     KVCDSVWAVYASRSYVQKHGRPRDIADLATHALIGFDGIMQGHRVARWLPAVVPDARI
                     VSRTSSMLGAVAAVKAGVGIAPLPTTLGDAEDTLVQVLPPVEALTRAWYLLTPADLRR
                     TPRIAAFVDWLLDDIPALREALIG"
     gene            complement(12203..13411)
                     /locus_tag="P5558_00490"
     CDS             complement(12203..13411)
                     /locus_tag="P5558_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22815.1"
                     /translation="MTNKGDDRLADRLQLVDDQKLRDVVRMLESTRERGAVEPILAAL
                     RPRLRRLRPERPLTLQRLLTVPLEGALATAGDGAWSFVVDRSRLGELHEAGLAGLPPG
                     LAAALETALAGRLAHETDAILEHGRTCWPAAAAALAAAAAVAGLPDRLAVEQRRVADL
                     LAIAPDLVPLLGRLPRPLQLLDDDDKGVLGEILALALAGSDDRCGTLLTLLLRSTRRP
                     AALVDDLLAAVEPAGQDRLRPLIDRILAEHRATLDSLLDGVVARPEAPVAQVAEAVER
                     VAEAVVDEAGAATALGRRAGAMAESRYLAAIQDLAMSPVVAGDAPTVLAGVRERETAA
                     RAVAKLGRAAGRLTRGETIERATREAVEALVESAGHLTERVVHVRVARLIEILAGADR
                     AWAHLRRHRA"
     gene            complement(13414..14529)
                     /locus_tag="P5558_00495"
     CDS             complement(13414..14529)
                     /locus_tag="P5558_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22816.1"
                     /translation="MTARRRLFSLKQRPSTFAEAGVVAPFTTPLLVQARMRLDYRGRL
                     EIVARNPTGADGMYVVPLAALNELYRLSLHDRALVERMQGLTPVSPLGIREIALSLSR
                     EGLAGPDAEAAAARSLQEEEERSLLTMLLLLEQLLQEADLGKIDWKNIDTGSSDARER
                     LRPYFKSLEPSMGIASGELITMLDELSGLVASIGHTKAPFKSLSDTTLEEVVELRRSI
                     AEWVRGERDDHAILAGLVVECATLTIDCTGPGLAKAKTIANSARRLLHARASDRQKVD
                     EVLTRPIWLLDGWRHLAALWRGGAEEPREAQRDILVELAELAPLVPLAADEWLGKPME
                     VRGNPELRRWVRLNEDWRTGHVLDRQAVIEQVLAEAL"
     gene            14721..15278
                     /locus_tag="P5558_00500"
     CDS             14721..15278
                     /locus_tag="P5558_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY22817.1"
                     /translation="MPSTITYLERLDPPRGAPLQPPCPGTEIVRALNPPVHFYRYLYA
                     TVGAPWLWNRRRLMDDATLAAIIQAPSTDLRVLWLHGVPAGYVELDFGEHPDVRLAFL
                     GLVPDFLGRGLGPWLLDWAIRHAFAKGATRLHLDTCDLDHPKALATYEAFGFQAYDRA
                     NSFETLLDDMELPAHCRDKPVLPLD"
     gene            15350..15589
                     /locus_tag="P5558_00505"
     CDS             15350..15589
                     /locus_tag="P5558_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRY22818.1"
                     /translation="MLSIGAFAAKTHLSALLERVARGEEVLITRHGKAIARLVPADTV
                     APVDHAALVARLKAAREKAELGDLDWRALRDEGRR"
     gene            15586..15999
                     /locus_tag="P5558_00510"
     CDS             15586..15999
                     /locus_tag="P5558_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY22819.1"
                     /translation="MSLVLDCSVALAWCFRDEASEAGDALLDDLPELAPLVPALWHLE
                     IGNVLLMAERRGRITSAQLQEHLALLRGLPLETDDEAPARTLPAVLALAGGHRLTAYD
                     AAYLELAVRRGASLATRDSALARAARSVGVKLRQA"
     gene            complement(15970..16245)
                     /locus_tag="P5558_00515"
     CDS             complement(15970..16245)
                     /locus_tag="P5558_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRY22820.1"
                     /translation="MAQPEQMGRISFDPSIMGGRPCIKGTRVTVATVLGLVADGLTVE
                     AILADYPYLVAEDIRAALAYAAWLAREEVGGDRCPPFCSGLPQLHPH"
     gene            complement(16328..17107)
                     /locus_tag="P5558_00520"
     CDS             complement(16328..17107)
                     /locus_tag="P5558_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY22821.1"
                     /translation="MRVTVLGCGTSAGVPQIGCDCAVCRSPDPRNKRLRCSILVETRG
                     QKILFDTGPDLRRQCLDAGISRLDALVFTHAHADHLHGLDDIRAINNVIGRPLPAHAH
                     ADVLQRIQERFPYAFGGGQDGRGGFWRPEIQPRPFTGDGPFTIGPALLLPLAHGHGRS
                     RVWGFRIGKFAYSTDADRLDEAYLEALAGIDVWLVDALRDKPHPSHSHLHRTLEWIAR
                     VRPKQAWLTHMNHEVDYATWQARLPEGVAPAHDGLVIELED"
     gene            complement(17107..17907)
                     /locus_tag="P5558_00525"
     CDS             complement(17107..17907)
                     /locus_tag="P5558_00525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRY22822.1"
                     /translation="MPESLPAFVDSHCHLDYPGLVAEVEAVVARANEAGVATLLTIAT
                     RLTSLPAVLELARRFPNVWVASGVHPHQAAEEAQSCDPAPFIASAADPRVVAIGECGL
                     DYFYDKSPREVQRASFAAQLEAARATGLPFIVHTRDADADTLAVLDEAAAAAPLRGVI
                     HCYSTSPQMGHEAVAMGLHLGLGGILTFRRSDELRATVRDLPRDRILLETDSPFLAPL
                     PHRGKRNEPAYIPHVAAVLAELWGTGTDEVARITTENFFRLFKKAARP"
     gene            complement(17900..19441)
                     /gene="metG"
                     /locus_tag="P5558_00530"
     CDS             complement(17900..19441)
                     /gene="metG"
                     /locus_tag="P5558_00530"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRY22823.1"
                     /translation="MAARAAYYITTPIYYVNDSPHIGHAYTTLACDALARFMRLDGRD
                     VRFLTGTDEHGQKVEKAARDRGMDPQAFTDEVSTRFRELARVMGFSQDDFIRTTEARH
                     KHAVQQLWTKLLDAGEIYLGAYAGWYSVRDEAFYAESELVAGKAPTGAPVEWVEEPSY
                     FFRLSAWQDRLLAYYEAQPDSVMPESRMNEVRSFVKGGLNDLSVSRTTFAWGVPVPND
                     SAHVMYVWLDALTNYISALGWPEDPDGLYARFWPCDVHVVGKDILRFHAVYWPAFLMA
                     AGLEPPRRIFAHGWWTNEGQKISKSVGNVIDPHALIDRYGLDATRYFLLREVPFGNDG
                     DFSHAAMLRRMNSDLANDYGNLCQRVLSMIARNCGAAVPRPGPILPADTALLDQAAGL
                     LETCRKLMARQASHAALEAIWAVIADANRYVDTQAPWALRKSDPERMATVLWVLAETI
                     RRVAILTQPFMPDASARILDQLAQPPELRDFASLDDRGALVAGTALPKPAGVFPRLVE
                     DAAGA"
     gene            complement(19478..20530)
                     /locus_tag="P5558_00535"
     CDS             complement(19478..20530)
                     /locus_tag="P5558_00535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRY22824.1"
                     /translation="MSEQGPPAPRANPHLFGHAAAEEVFERAWRSGRLPHAWLLQGPP
                     GIGKATLAYRLARRILAGPGMVDPNDPESAIFRRIAHGSEPDLFALANEVNPRSNRMS
                     GVILVGQARDLTTALHATAVGRHGRVIVVDAADDLNRNTANALLKLIEEPPAGVVLLL
                     VCHAPGRVPRTILSRCARLRLQPLAPEPMAKALAAAGLAAPGEASPALLALAKGAPGR
                     YAMLGAAGYLEHYAALLDALIAGRDDRRPLVEAADRLAGIGNAGLVVELLALILRRAA
                     EQATRGAIDPELAPGEASRLAALVGGRRLDRWMGLWDKLRDLPGELDHLNLDPRQTLY
                     LALASLAGATRERLAG"
     gene            complement(20527..21147)
                     /gene="tmk"
                     /locus_tag="P5558_00540"
     CDS             complement(20527..21147)
                     /gene="tmk"
                     /locus_tag="P5558_00540"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRY22825.1"
                     /translation="MTFEGGEGAGKTTQLQALRHLLEARGATVTATREPGGTPGAEAI
                     RDLVLKGPAERWSHGVEALLMTAARLDHVERLVEPALAAGSWVLSDRFSDSTRVYQGI
                     AGGLGTKRIDDLHDLFLPGARPDLTLLLDLPVSAGLARRAKAGGGSRFEALDTAFHER
                     VRAGFLELAGAEPGRFVVIDATEPAEAVAGRIEATILARFADRLAG"
     gene            complement(21177..22319)
                     /locus_tag="P5558_00545"
     CDS             complement(21177..22319)
                     /locus_tag="P5558_00545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRY22826.1"
                     /translation="MMRLLAALVLLATAIAAGPARAQTAFETAARAAILIDYRSGDVL
                     FEKNPDEALPPASMSKLMTALVAFEELEAGNLQLDEELPVSERAWRMGGSRMFLEVGT
                     RATVHELLQGIIVQSGNDACVVIAEALSGSEEAFADRLTKRGIEIGLTNTTLRNATGW
                     PDPEHLMSVRDLSIVARQIISRFPQYYQYYSQLEFEFNGINQHNRNPLLQGGIPGVDG
                     MKTGYTQEAGYGLVASAERDGRRLILVVAGLESSRQRRSESERLLEYGFRHFQEYRLF
                     EPDAVVAEAPVWQGAEPTVPLVGADVVSITLTRAARDSLNVKVVYDSPVTAPVEAGQV
                     VGQIEMTADGMPTRVLPLVAARPVERAGILGRMTGTLEYLIWGVPG"
     gene            complement(22363..23226)
                     /locus_tag="P5558_00550"
     CDS             complement(22363..23226)
                     /locus_tag="P5558_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRY22827.1"
                     /translation="MTGRRTGPFLALALAALLLAACSGPRSTPPDLDPLATTPHGTYK
                     LGQPYRINGIWYFPEFDPAYDETGIASWYGDDFHGLPTANGEVFDMRQPSAAHPTLPL
                     PSVVEVTNLDNGRKARLRVNDRGPFHDNRLIDLSRAAARELGFEGKGLARVRVRFVSL
                     LPAHGVPPVAGTTTRPATVVASAVPAAAPVIRSTPARAPTRSPVRPPARASGCGADDH
                     FVQVAAFSEPANAAALERQLHGLGPVDVQTAPPVSRVRLGPYASRSDAFGTLARVHGM
                     GYRDALVVSCS"
     gene            complement(23223..24221)
                     /locus_tag="P5558_00555"
     CDS             complement(23223..24221)
                     /locus_tag="P5558_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRY22828.1"
                     /translation="MALRSCPIMMGGGRRLARLASALVLGLGLAAPAMAATEFAAWLD
                     GLRSEARARGVSDRTLDEALAGVEPIPRVIELDQRQPEGRMSFVEYKGKVISEARISQ
                     GRRLLAEHGPMLRQVEARYGVPAEIIVALWGIESSYGEFKGRFPVVASLATLAHDGRR
                     ADFFRGELLSALQILEAGDIEPDAMFGSWAGAMGQSQFMPSTYLGYAVDGNGDGRRDI
                     WGTHADIFASMANYLSKMGWNPEYIWGREVKAGSGVASAGLDEERPLATWQARGVRKL
                     DGSALPAVAIDASLLKMDDGSGPSYLVYDNFRVLMRWNRSTYFATSVGLLADAIRA"
     gene            complement(24301..24642)
                     /locus_tag="P5558_00560"
     CDS             complement(24301..24642)
                     /locus_tag="P5558_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22829.1"
                     /translation="MVEQVARGRLKVAAVAALGLATVGASAPANAAGKSTTMQIRVRV
                     VESCQIRIDNRLGRPLQFCGGPGAIPPIATIPKITFDLPPLPPLPRIEWPASRLAASS
                     LKRVRFVTFTY"
     gene            complement(24761..25201)
                     /locus_tag="P5558_00565"
     CDS             complement(24761..25201)
                     /locus_tag="P5558_00565"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HRY22830.1"
                     /translation="MRRLIRIWCCAALLCAIAARPALAVCQFATTALAFGVVDVARGA
                     DSTGEIAVSCQTATSFEVAIVGNGSPGNRYLSGPDGARLTYDVYPDATRSVPWTDGSG
                     SGAVVAASGNGDETTRLSIYGRVPIQKAVPAGQYLDSLTVSVTF"
     gene            complement(25189..27837)
                     /locus_tag="P5558_00570"
     CDS             complement(25189..27837)
                     /locus_tag="P5558_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HRY22831.1"
                     /translation="MPGRLHRGLTGSATIGLLLVLLGVDRTPLAGPGGATAPARATSA
                     EGPPDTARYAVALTSLILQNHAPSGMPALPPTARPAARAMPPRPAPRPAPKPAPPVLV
                     AAADPPAAPLPEQLEALDAIEPGGITYRRDPLALPLLESERWTEWVLSVELNGEPVSD
                     GVIFIEDPVSGRLAVELATAEAWRLIIDRDAILTFEGVPFYPLDAIPGAVAVLDPAAL
                     VVAIEAPPDVFGLFAMDLEGLERTPPTPGTGAYFDYDLLLQGGGDIDDQLDGLFEAGA
                     FSHGNVFLNNFRVEDAGDEAEIDRLETTFFHDFPEHRATFRAGDSLTVGGAFAQPVRF
                     GGLQWSTNFATDPAFVTFPLPSIGGLAEQQSVVDVIVDNLTSITRTVPPGPFAIDNVP
                     VVTGAGEVQLRVTDLLGRERLVTQSYYVSTRLLKAGLHDFSYEAGFKRNDFGERSFAY
                     GDALGTATHRYGFTDTVTGEAHLEAGQDGASLVGGGALLLGPYGLLSGGLGGSVDSGA
                     DEGWLGQLAYEYVGRRLSLGARTRFTSDDFRQASSDDGRTRRVDQLNIGYDTGSIGRL
                     GLLFLNRDRINRDDDQSLAASYSLPIGPGALVVNAAQTLSPDSDFALTAAYSIPLGSN
                     RSMTAIADAGDGRNRGRLQYTRTRGASELGLDYRLASEIGDDARPVDAWLGYQASKIG
                     TEVAYERFSGDNRLRLGADGSLALVDGNFGLTRRIGRAFGLVDLPGFPDVRVYVDNRE
                     AGRTDADGRLLLPDLSPYERNRISLAVEDLPIDAELTIAETAAVPFDRSGMTIDFGIV
                     AVQRATATLRDRADRPLPPGLVLADETGGLSGLVARDGFTQLTGPLHAKAYLIGEQGG
                     RRFVCAVPAFAGTDPLPDLGEIRCAD"
     gene            complement(27839..28549)
                     /locus_tag="P5558_00575"
     CDS             complement(27839..28549)
                     /locus_tag="P5558_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HRY22832.1"
                     /translation="MRRAHRRAVLALVIGFVMVPWPAIATSLNVAPTRIDLGPASRTG
                     SVTLNNTGEAATTVQVETFAWHHGNSVAELEPTRGLLAVPAVFNLDRGARQVIRVATR
                     EPASTEVETAYRLVITEVPVARDPAAAGVRFALRLSLPVFITPPGAAPVPDWRLVQGA
                     DGPALEVRNAGTAHLHVSRLSLRAAAGGDPLALIDTPSYVLAGNTQRWPLPSLQGRRD
                     LVVEAATNLGDLVVELPN"
     gene            complement(28558..29070)
                     /locus_tag="P5558_00580"
     CDS             complement(28558..29070)
                     /locus_tag="P5558_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HRY22833.1"
                     /translation="MATRHHRRRHPCARAMAALALAASPVAAPNGAHAATATDQLTVT
                     ATVQSGCTLTGGTLDFGNYVSGQTSNLDANGRIGFANCSGLLTFELDGGQSGNVNNRA
                     MRSSGNTLTYQIYRNSVRNAVWGQGQNALQLQLLQPQSGEAVVYGRIPGGQAVPGGAY
                     VDIVNITLTF"
     gene            29255..29569
                     /locus_tag="P5558_00585"
     CDS             29255..29569
                     /locus_tag="P5558_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRY22834.1"
                     /translation="MDKPLRITFDNLPHSEAIEANVRSRMARLDALCDRMTGARVVVG
                     SPHRSLTKGKTYAVRIELALPGRDLVVSREPVGDLHEAMAAAFATARRRLRGFVERSR
                     GA"
     gene            complement(29596..31245)
                     /locus_tag="P5558_00590"
     CDS             complement(29596..31245)
                     /locus_tag="P5558_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY22835.1"
                     /translation="MSDSRPATPTPTDPDLAAESERLRAELAAAQRRLEERGLFLAIM
                     SHELREPMNGVLGMARLLRDTALDAEQAGYVEAVIGSAESLITLVNDILDLARIDAGR
                     STFTLVDFAIRPFLERLALVFRQRAEQKGIAFAIQLAPDLPEVVRGDPGRLRQLLTNI
                     VTNGIKFTSEGEVRLAVAPADAAAGRLGLAIAVSDTGIGIPEDRRADFFSPFAQADAQ
                     TARLFGGSGLGLMIAQRLAATMHGRIVIAPPVGRGTTFEIHLELEPPLATAPRRDRAA
                     IAGARLLIADAQPRTARIMAELARLWGLEVRVTGSAAEAQAVLEESADRGAAFDFAVI
                     DRTLPDRRGDSLGPILRAAPGQAGLKLILLVSAGVRGDAAKVQALGFDAYLQKPVTAS
                     TLLDCLQQLHGSAGQGELLTVHALGEARSQKLRLLIADDNPVNCKLAAIMLSKAGHEV
                     QIVTNGQEALDAVTEIPFDAVLMDVQMPVMGGLEATRRIRALPDPGAAGLPIIAITAN
                     AMRGDDDDCFAAGMNGYVTKPIDRATLLATVERLARPAPAA"
     gene            31385..31765
                     /locus_tag="P5558_00595"
     CDS             31385..31765
                     /locus_tag="P5558_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22836.1"
                     /translation="MAIDWCEAARLVAAGLPSREIIARVGCSRSQLHRRRTSCSLFKA
                     LVAAHAAGTAADRPPEDAASIEAKVRRKVEDEVAEGNLKVALWLAERLRMFTPDGEDS
                     PEDTIQRLMESMTPLERAAFSRAE"
     gene            complement(31774..32115)
                     /locus_tag="P5558_00600"
     CDS             complement(31774..32115)
                     /locus_tag="P5558_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22837.1"
                     /translation="MSKTASAARGLEPSAVPDQAASAPENDVPTLELTDLVQDSNGEI
                     VLFNDSHLPALAVRALSQPIDTGAVGQHVTAGGDDVTGFRFVAFDDGTRLYYQDSLHL
                     VVLDDLHAAAT"
     gene            complement(32278..33126)
                     /gene="speE"
                     /locus_tag="P5558_00605"
     CDS             complement(32278..33126)
                     /gene="speE"
                     /locus_tag="P5558_00605"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRY22838.1"
                     /translation="MEWFAETLHSDWQQRMKVEKILYHDQTEHQDLIVFETGRWGTVL
                     ALDGVVQTTTGDEFAYHEMLAHPAILAHGRVRSVCIVGGGDGGTAREVMKHATVERVL
                     QAELDPGVIEFCRKFLPTLSDGAFDDPRLELAFGDAAAFMAKDGESFDVILVDSTDPI
                     GPGAALFTESFYRDCRRRLNEGGILVTQAGNPALAGRELADVMARKRAAGFADVDFLL
                     TVVPTYVGGFMALGWGSDDATRRRVRSDTLRARPIPEGLRYYTPEVHVAAFAHPAWVE
                     QVARGG"
     gene            complement(33169..34896)
                     /gene="ilvD"
                     /locus_tag="P5558_00610"
     CDS             complement(33169..34896)
                     /gene="ilvD"
                     /locus_tag="P5558_00610"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRY22839.1"
                     /translation="MTSVTIYDKSRLPSRHATVGPDRAPHRSYYYAMGLSAADMARPF
                     VGVVTTWNEAAPCNIALGRQAQVVKQGVARAGGTPREFTTITVTDGIAMGHQGMKSSL
                     VSREVIADSTELTMRGHAYDAMVGLAGCDKSLPGLMMAMVRLNVPAVFMYGGSILPGR
                     FRGRDVTIQDVFEAVGQHAVGKMSDADLEELEQVACPSAGSCGGQFTANTMACVSEAI
                     GLALPGSAGTPAPMEARDRWAEASGEAVMRLLKDNIRPRDIVTLKSLENAARVVAASG
                     GSTNGALHLPAIAHEAGIDFDLHAVAAIFRETPYIADLKPAGKYVMKDLAEAGGVPLL
                     MKALLDGGYLHGDCITVTGRTIAENLADVAFDTKQVVVRPTSDPITTWGGVVGLRGSL
                     ASEGAMVKVAGLPSLKFRGPARVFDCEEDAMAAVQAGKYVAGDVIVIRYEGPRGGPGM
                     REMLGVTSAIYGQGMGDKVALITDGRFSGATRGFCIGHVGPEAAVGGPIGLLEDGDTI
                     AIDAEAGTLDLELDEATLDARRAKWQPRQSIYGAGSLWKYAQTVGSAEKGAVTHPGGA
                     AERHCYADL"
     gene            35074..36066
                     /locus_tag="P5558_00615"
     CDS             35074..36066
                     /locus_tag="P5558_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRY22840.1"
                     /translation="MAGGSDMVRRRVRRGRNHRVLLTPIQPFATTGLEEPTSPVRTRR
                     RRPPDLGWVRRILRPAFGHFVHDQGFVLAGYIAFTAIFAFFPFLIVMLSIAGWAGQGE
                     AAGQFITLTLELLPNEVASVLQPAIAEIRSAPHGTIITLAIPVTLWFASSGLESLRHA
                     LNMAHEVADPPSFWWNRLASMALTVASATVILAAMVLMVGLPIAENILAWLAEHQALE
                     ERMGLIMRYGIGMALLLLLTFSLYRLLPNRRLTLLDVLPGTLVSVTFWLGATKLYSTY
                     LSSIGRYSIIYGSLGGVILTLFFFYVMACIFIFGAQLNAAIMRERALQRERRAA"
     gene            36080..36991
                     /locus_tag="P5558_00620"
     CDS             36080..36991
                     /locus_tag="P5558_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRY22841.1"
                     /translation="MTLGAARVEAAVLRHSLWLTVAIAAVGVVFGILSGSLAIIFDGV
                     ASSINAAMTFLSLAVARLLAREGSRRFQFGYWHFEPLALALNGALLVMFCFYGFVTGV
                     SDLLAGGRDLDFGWAMAYAALSTVASLVTWLYERWRNRTIRSELIGLDMQSWLMATAV
                     SAALLVAFGYALMVEGTALAWTAALADPAVLALVSLGLAFMPIAVLHQAWQDIFQMAP
                     GSLDAAVEAAARRAVERHGLATFTSYVARAGRATFIEIHLVLPADYPIDSVATLDRIR
                     REIAEELGPARPERWLTVAFTGDATLI"
     gene            complement(37039..37554)
                     /locus_tag="P5558_00625"
     CDS             complement(37039..37554)
                     /locus_tag="P5558_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRY22842.1"
                     /translation="MIFDSWAGIGRVLLVGTLAYVALVVVLRISGKRTLTKLNAFDLV
                     VTVALGSTLATVLLSKTVPLAEGIAALTLLVGLQFLITWLSVRWPSFRRLVKSEPTLL
                     MLRGSPLDRAMREQRITLEEVTAAVRSRGFADMAEAGAVVLETDGTISVLGQVPEPLP
                     DRSTLEPTARL"
     gene            37706..38518
                     /gene="fabI"
                     /locus_tag="P5558_00630"
     CDS             37706..38518
                     /gene="fabI"
                     /locus_tag="P5558_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRY22843.1"
                     /translation="MTTGAPLMAGKRGLIMGIANERSLAWGIARAVAGQGAELAVTYQ
                     GDALKKRVTPLAAELGCHFVEPADVTEPASMEALFTTIEQRWGGLDFAVHAIAFSDKE
                     ELKGRYVDTSAENFQRTMLISCYSFTDLARRAAALMQPGGSLLTLTYYGAEKVVPHYN
                     VMGVAKAALEASVRYLAADLGGQGIRVNAVSAGPVKTLAASGIGDFRYILKWNELNAP
                     LKRNTSLGDVGGAGVYLLSDLASGTTGEVLHVDSGYHVVGMKAVDAPDIATV"
     gene            38526..39599
                     /gene="aroC"
                     /locus_tag="P5558_00635"
     CDS             38526..39599
                     /gene="aroC"
                     /locus_tag="P5558_00635"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRY22844.1"
                     /translation="MAGSSFGTLFRFTTWGESHGAAIGCVVDGVPSRLELAEADIQHW
                     LDRRRPGQSRWTTQRQEPDRVEILSGVFEGLTTGTPISLLIRNEDQRSKDYGAIARSY
                     RPGHADFTYDAKYGIRDYRGGGRSSARETAMRVAAGAIARKILGPQIAIRGCMVQMGA
                     DRIDRQRFDPAAIEANPFWCPDKEAVARWEKLLGRVRKAGSSIGAVVEVVASGVPAGL
                     GEPIYDKLDADLARAMMSINAVKGVEIGAGMAAAALSGEENADEMRKGPDGPIFLSNQ
                     AGGILGGISTGQDIVVRFAVKPTSSILTPRRSVTADGDETEIVTKGRHDPCVGIRAVP
                     VGEAMLAIVLADHLLRHRGIFGG"
     gene            39733..40191
                     /locus_tag="P5558_00640"
     CDS             39733..40191
                     /locus_tag="P5558_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22845.1"
                     /translation="MQWVHKRSLPIPALTITDPALIVAYRYWAERKDGAHLPPRCSVD
                     TPIFRLLVPDALWLGTGAYAGRLAPFLGATQVHVPSMTLEESLRLDMDAAAVTGVPLL
                     QLVELKIRGRPLIYQQLLLPTSDDGCKVNEVLEVSKLGALQLMGSRPTSA"
     gene            40474..41649
                     /locus_tag="P5558_00645"
     CDS             40474..41649
                     /locus_tag="P5558_00645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY22846.1"
                     /translation="MRLAAAMGRLGTESAFEVLARATALKAQGRDIINLGIGQPDFPT
                     PPHIVEAGVKALRDGHHGYTPANGILPLREAVVRDMAKRRGVEVDPASVVIVPGGKVT
                     MAFAMLMFGEPGAEIMYPDPGFPIYRSMIEFTGAEAVPMALHESKGFAFSAEEVLGQI
                     TDRTRLIIVNSPANPTGGVVPKAELDKFVHGLADFPEVAVMSDEIYSRMCYDGVPHAS
                     LTTYPEIADRLILLDGWSKTYAMTGWRLGWGVWPGKLAEHATRLAINVNSCVNAPTQY
                     AGIAALDGPQEAVGSMVEAFDRRRAVIVERLNALPGFRCAVPGGAFYAFPNIEGTGLG
                     SRAMQDGLLNDAGVAIISGTSFGVYGEGYVRFSYAASIEEIEKAMDRIAGFLAKRAA"
     gene            41757..>43003
                     /locus_tag="P5558_00650"
     CDS             41757..>43003
                     /locus_tag="P5558_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22847.1"
                     /translation="MPIEQSALAAIRSELGQVLWDAPLYQGIFNVQLARAPTRVRFGF
                     QTFWPCYARVEVYRMVTGELDLDMEKENLLRVGYELFGSPKVSHNVLITELGQEQRFW
                     YRISVPRKTPDFPDAKGFVRSRGEFFTPRRNTIVTVDRIHVMDDSDTSGEGELTFSLG
                     LYNASDPRERLVEPRISPKQGVSSGDDYNRPFGAGGQSIGRAPDFIGVYVFGVDDDAT
                     FWGAPWYGLGIVGMRPPATMPDRTFTESRDYADMTDALHVFATGAKPGRQEGRFDFSS
                     VEGRLHFSVHGRFVTEVVDTLGERITPPWKAFFKTSMAIGTGGKPAGLVGLPAGAIGV
                     ALDPEGRLQLGEGGSLDRWRAAEGPAFQALVAKATRAGALVLVGRDGDGVAHVARLLE
                     PHRDMPCWTAIGRTTDGPPALLE"
BASE COUNT         6187 a        14650 c        15758 g         6408 t
ORIGIN      
        1 cgagccattc gcgcaccggt gccgggagag ccggccagtg ggcgggctcg gccaggaggc
       61 ggcggacgcg atgggcgagc tggctggtga gcggcagccg accgccggca taggccggga
      121 tcttgggctc cctggcacct ttggcgagcg aggcgatcac ctcgttctgg cggatgccct
      181 cgaaggcgag cacgcggccg gcgaagagga agctgtcgcc cggggtcagc atcgagacga
      241 aatactcctc gatctcgccg agcgaccggc cccggcccct gcccccaccc aggcggacgc
      301 gcatcaccgg ggcctcgacg atggtgccga tgttcatgcg gtagccgcgc accagccgac
      361 ggtcggtgag ctgccagagg ccatccggcc cctgctcgag gcgtcgatag cgctcgtact
      421 ggcgcagcgc gtagccgccg gtggcgacga agtcgagcac ggcgtcgaag tcggctctgg
      481 gcagctccgc atagggctgg gcgcggcgca cctcgtcgaa gagggcctcg gcgtcgaagg
      541 ggccgctgca ggccacaccg gtgacgtgct gggcgagcac gtcgagggcg ggcccgagcg
      601 gcggggcgcc gtccagctcg cccgcctcga tcgcctcgat ggcggcgatg cattccagca
      661 cctcgaagcg attggccggc accagcacgg cgcgcgacgc ctcgtcgagc cggtggttgg
      721 cgcggccgat ccgctgcagg aggcggctcg cccccttggg ggcgccgatc tgcaggacga
      781 ggtcgacgtc gccccagtcg atgccgaggt cgagcgagga ggtgcagacg acgccgcgca
      841 gcgccccacg cgccatggcg gcctcgacct tgcggcgctg ctcgacggcg aggctgccgt
      901 gatggagcgc tatcggcagg ttctcgctgt tgatgcgcca gagggcggca aagaagagct
      961 cggcgagggc gcgggtattg acgaagacga ggccggtgcg cacggccaaa aggcgggcat
     1021 agacggcctc ggccgaatag agggcggcat ggccggacca gggcatggcg gcctcggcgt
     1081 cggcgatgac gatctcgggg gcggccccct gctcggcctg gacgatgcgg gcgcgcgtcg
     1141 ggtcgggggc gaggaagcgg agcagggccg gtgggtcggc gacggtggcg gagaggccga
     1201 cgaagcgggc ctcgggtgcg agtgaccgca gccgcgccat gccgaggctg agcagcgcac
     1261 cccgcttgct ctcggcgatg ctgtgcagct cgtcgaggat gacgcaggcg aggttctgga
     1321 agtaggtcgc ggcattctcg tagctgagca gcagcgccag ggattccggc gtggtgagca
     1381 ggatctgcgg cgggtgggtg cgctggcgct ggcggcggtt ggccggggtg tcgccggtgc
     1441 ggacggcgta ggtgacgccg aggcccatct cggccagcgg ggcctcgagg ttgcgggcga
     1501 tgtcggtggt cagcgccttc aagggcgaga cgtagagcgt gtgcaggccc gtggtcggcc
     1561 gctcggcgag gtcgacgagg ctcggcagga agcccgccag cgtcttgccg ccgcccgtgg
     1621 gcgcgatcag cagcgtgtcg cgcccggccc gggcctccac cagcatggcg agctggtggg
     1681 cgcgcggccg ccagccgcgg ctggcgaacc agccggcgaa gggctgcggc aaatcgctgg
     1741 cggtcgtgga catccgggcc tttggctcct accaacgggc cgaacctaga ccccgtatcc
     1801 tgtgtggcaa tcgatggacg aggaaacgct tcgcgccagc aaggcctggc cgatcacgga
     1861 agcactggca ctgctgcagc gggtcgagag gaagccgccg gccaagggct tcgtgctctt
     1921 cgagaccggc tacggcccct cggggctgcc gcatatcggc acgttcgccg aggtcttccg
     1981 cacgaccctg atccgccagg cgttccaccg gctctcggac gtgccgacga agctctatgc
     2041 cttctcggac gacatggacg ggctcaggaa gctgccggac aacatcccgc accccgaggt
     2101 gatcctgccc ttcctgcaga agcccttgac cgccattccc gaccccttcg gcacgcacga
     2161 gagctacggg ctcaacatga acgcgcggct gcgggcgttc ctcgaccgct tcggcttcga
     2221 ctacgagttc aagagcgcca ccgaggagta caggaagggc accttcaacg aggtgctgct
     2281 gcgggtgctc gagcgtcacg aggcggtggc cgacgtggtc cgcccgcatc tcggacccga
     2341 gcggcgggcg agctacagcc cgatcctgcc gatctcgccg aagtccgggc gcgtgctgca
     2401 ggtggcgatc gaggagtgcc ggccggccac gggcaccgtg gtctttcggg acgaggacgg
     2461 cacgctcacc gaggtgccgg tcacggacgg gcgctgcaag ctgcagtggc gggccgactg
     2521 ggcgctgcgc tggacggcgc tcgaggtcga ctacgagatg tcgggcaagg acctgatcga
     2581 ttcggtcaag ctcggcagcc agatctgccg ggtgatgggc gggacaccgc ccttgggctt
     2641 cacttatgag ctcttcctcg acgagaacgg cgagaagatc tcgaagtcga ggggcaacgg
     2701 catcaccatc gacgagtggc tgcgctacgc cacgcccgag agcctgatgc tctttctcta
     2761 tcgcgacccg aagaaggcga agaagctcta tttcgacgcg atcccccgga acgtcgacga
     2821 ctacgagagc ttcgtcgccg ccttcgaggg ccaggtcgac aaggagaagc tgggcaaccc
     2881 ggtctggcac atccacgacg gcaagccgcc cgagggtgcg gtgccgatca actactcgat
     2941 gctgctcaac ctggcgtcgg tggtgaacgc cgaggacccg gaggtgctct ggggcttcat
     3001 ccggcagtac agcccaaagc tcacgccgga aagcgagccg cgcctcgccc gcctcgtcga
     3061 ccatgccatc gcctactacc aggaccgggt gcggccggag aagcggcacc gcgagccgga
     3121 tgcgaccgag cgggcggcca tgctggagct ggaggcggcg ctggccgggc tcgccgaggg
     3181 cacgcccggc gaggccatcc agaacgaggt ctacgagatc ggcaagcggc acgggttcga
     3241 gagccttcgg gactggttca aagccctcta cgaggtgctg ctcggccagg agcaggggcc
     3301 gcgcttcggc tcgttcgtgg cgctctacgg cgtcgccaac acgcggcggc tgatccggga
     3361 agcgctcggc ctgccggcgg cggccgttcc gggtgagtga gttctccatc gggctcgagg
     3421 cccgcacgct ggcgctcgcg gtgctcgatg cggcgctcaa cgagcgcgcc ccggccgagg
     3481 cgcggttcgc cgccgacccg gcgacggagc ggctcgagcc gcgcgaccgg gccttcgccc
     3541 ggctgctcta cacgaccgtg ctgcgccagc gggccgtgct ggacgcggcg ctgctgccgc
     3601 ttctgcgcca tccgccgccg cacaagagcg ggatgaacct gctgcggctg ggggcggccc
     3661 agctcctgct gctgggcacg ccgccgcacg cggcggtcgg gctgaccgtc gagctcgcga
     3721 agaaacggcg gatcgtgccg ccggccctgg tcaatgcggt gctgcgccgg ctggtcggca
     3781 cgaagccgcc ggtggtcgac ccgctcgcca acctgcctga ctggctgcgg gcgagctgga
     3841 gcacggccta tggcgccgag acggcggcgg cgatcgccac cgcactcgcc aaggagccgc
     3901 cgctcgatct ctcggtcaag acggatgcgg ccggctgggc cgcccggctg ggcggcgagg
     3961 tggtcgggcc ggcgacggtg cgtctccgcg aggccggggc cgtcgagcgg ctgccgggct
     4021 accgggcggg tgcctggtgg gtgcaggacc tggcggcgag cctgccggcc cggctggtcg
     4081 gggcgaagcc cggcatggcc gtgctcgatc tctgcgccgc gccgggtggc aagacggcgc
     4141 agctcgtcca ggccggggcg aaggtgaccg cggtcgagcg cgagcgcggg cgggcggagc
     4201 ggctgaggga gaacctcgca cggctcgacc tcgaggccga gatcgtcgtc gcggacgcca
     4261 tggactacgc gccggcaaca ccgttcgacg cggtgctgct ggatgcgccg tgctcggcca
     4321 ccggcaccat ccgccgccat ccggacatcc tctggagcaa gaccgaggcg gacgtgcagc
     4381 gcctcgccgg gctgcagggc cggctgctgg atgcggcggc ccggctcgtc cggccgggcg
     4441 ggacgctggt ccatgcggtc tgctcgctcg agcccgagga gggcccggcg gtggtcgagg
     4501 cgtttctctc acgccacccg ggcttcgtcc gccagcccat agccgaggcc gagtgcggct
     4561 tcgccccgac gccttcgggg gatctccgca cccatccggc cctgctggcc gagaagggcg
     4621 gcatggacgg tttcttcatc gcccgattca ccaagagccg ttgagcacgc caatgtcgaa
     4681 gagcctgccc cgccgtgctt tgctctccgt cttcgacaag accgggctgg tcgagctcgc
     4741 caccgcgctc gccgggcatg gcgtcgagct ggtctcgacc ggcgggacgg cgcgggccct
     4801 gcgcgaggct gggctcgagg tggtggacgt ctcggcgatc agcggccggc cggagatcct
     4861 ggacggccgg gtcaagacgc tgcagccgga gatccacggc ggcattctcg cccgccgcgc
     4921 cgacccggcg cacatggcgg tgctggccga gcacgggatc gccacgatcg atctcgtcgt
     4981 cgtcaatctc taccctttcg cccggaccct ggcgtcgggg gcggacttcg ccacctgcat
     5041 cgagaacatc gacatcggcg gaccggcgat gatccgggcc gccgccaaga atcacgagga
     5101 cgtggcgatc ctcaccgatc cggcggacta cccgaggctc gtcgaggcgc tcgccgctgg
     5161 cggcacggat ggcgcgctcc ggcaggagct ggcgcgcaag gccttcgccc acacggctgc
     5221 ctatgacgcg gcgatcgcga cgtggctcga cgggctggac ggctctcccc tgcccgaccc
     5281 gctgctggtc gcggcaccgc tgcagcagac gcttcgctac ggcgagaacc cgcaccaggc
     5341 gggcgctctt catgcctggc cgcagggccg gccgggcgtg gcgctggccc gccagctcca
     5401 gggcaaggag ctctcctaca acaacttgca ggacgccgat gcggcggtcg agctggtggc
     5461 cggcttcacc cggcccacga tcgtgatcgt caagcacacc aacccctgcg gtgtcgccag
     5521 cgctgcgacg ctgcacgagg cctgggagcg ggcgctggcc gccgacccgg tgagcgcctt
     5581 cggcggcatc gtcgcgagca accggccggt cgacgcggcg accgccgagg ggctggccgg
     5641 gctcttcctc gaggtggtgg tggcgccggg cttcgcgccc gaggcgctgg agcggctggc
     5701 cgcgaaggcc aatctccggc tgctcgtgct cgaccgcctg ccgggcccgg gcgaggcgat
     5761 gctcgacgtc cgcagcatcg gcggcgggct cctcgtgcag gcgcgggata ccaccaccag
     5821 cgaccccgcg agctgggaga cggtgaccag gcgggcgccc acggccgagg agcgggccgc
     5881 cctcgccttc gcctggaccg tctgccggca ggtgcgctcg aacgccatcg tggtggcggc
     5941 tggggagacg acgctgggca tcggcggcgg ccagaccagt cgcgtcgagg ccgtcgagct
     6001 cgcctgccgc ggccttcgcg tcgagcccgc ggcggggccg ctggtgctcg ccagcgacgc
     6061 cttcttcccc ttcgcggacg ggatcgagcg ggcggccggg gcggggatcc gggcggtcgt
     6121 ccagcccggc ggcagcctgc gcgacggcga ggtgatcgcc gcctgcgacg cgcacgacat
     6181 cgccatggtc acgaccggaa ggcgccactt caagcactga ggcgagtggg ccacaggcgt
     6241 cccctcgccg accccggatt aacccctccc taacgccgga gggtcgatca tccagactcc
     6301 accgactgct cccgccgtgg cgtgcggggg cgcaacggag tccggaacat ggtcgaccgc
     6361 gccgtcgtct atttcacctt cgtgctcttc gtcgccgcct ggctgctcga ctcgcccgtg
     6421 cccatggcca tcgccccctg aagcccgccg ggccgacctg ctggcatcgc ccgggaccgg
     6481 gtggtaagca gcgtggcggt ccaggaggag gcgggcatgg ccgaggcgca gattctcgtc
     6541 gtcgacgtcg gcggcaacaa catcaagatc aagcacgcgg cccatgtcga gaagcgcaag
     6601 acgaagtccg ggccggagat gacaccggag cgaatggtcg acgcggtgcg caagctcgcc
     6661 ggcgactggc gcttcgacca tgtcaccatc ggctatccgg gcccggtgcg cgagggccgg
     6721 ctggtgctcg agccgcacaa tctgggccgg ggctggaccg atttcgactt cgccgccgcc
     6781 ttcgaccggc cggtcaaggt ggtcaacgac gcggtgatgc aggccatcgg ctcctatgcc
     6841 ggcggcaaga tgctgttcct tggcctcggc accggcctgg gtgcggcgct ggtcggcgac
     6901 catgtcgccg tgccgctcga agtggcgcac ctgccctacc gcaagaagtt caccttcgag
     6961 gactgcgtcg gcaagcgcgg cctcgaccgg atgaagcaga agcgctggga gaaggcggtc
     7021 cacgacgtgg tggagcggct gcggcgcgcg ctcgtcgccg actacgtggt gctgggtggc
     7081 ggcaacacca agcatctcca ggccatgcca cagcacagcc agccgggcga caacgagaac
     7141 gccttcctcg gtggcgtgcg catctggcag gagcatttcc gcgtcctctg accgccccgt
     7201 tccgcgcgcc gccgtgccgg gccgccccgg cggccccggc cgcaacgccc ggctgaccag
     7261 cgacccgctg cccggcctgc tgcgcgccat cgccattccg gccggcacgg gcctgctgtt
     7321 ccagacgctc ttcaacatcg tcgacagctt ctgggcgggc ttcgtctcga ccgcggccct
     7381 gaccgcgctg ggtgcctcct tcccggtctt cttcctcgtg atcgtgctct cggtcggcat
     7441 cggcatggcg gcgacggcgc tgatcgccaa ctgcctcggc cgggaggatc aggagggcgg
     7501 ccgccggctc ttcggccagg cggtcttcct gacgctcgtc ggcggggtga tcgtcgccct
     7561 ctacgggctt ttcgcgatcc ggcccctgct ccgcttcctc ggcacctccg gcgagagcct
     7621 cgacgcgggg gtcgattatc tcctgccgat catgctgctc tcggtcgtct tcctgctcaa
     7681 ccagacgctc aacggctggc tgacggcgca gggcgacagc cgcagcttca ggaacgcgct
     7741 ggcgctgggc tgcgccctga atgtcgtgct cgacccgctc ctcatgttcg gggcgttcgg
     7801 cgtgccgggc ctcggcgtgc tcgggatcgc catcgcgacc gtcctgatcc aggcgatgca
     7861 gctcgtctat ctcgtccatc gtgcccggcg gagccccatg ggccttcggc tccgcctcgc
     7921 ggacatccgg ccggacccga aggtgctgcg cgatttcctc cgccaggccg tgccggtcac
     7981 cgtcagcatc ggtgcgaccg gcttcgggct cttctgcatc acctggttca tgggccggca
     8041 cggcgaggcg gcgatcgccg cctatggcat cggcctgcgc atcgagcagc tcgtcatgct
     8101 gccgatgatc ggcctgaaca ccgcggcgct gacgctggtc ggccagaacc acggcgccgg
     8161 gcggcccgac cgcgtgcgcg agacggcgcg gctggtcatg ctccacgggg ctgccgtgat
     8221 ggcgctgggg gcggtcgtcg tctggctgac gcgggcgcac ctcatcggca tcttcagcga
     8281 cgacccggac gtggtccggc tgggggcggc ctatctcggc ttcgccgtct tcaacttcaa
     8341 cgcctatgcg atcctgatcc tcggcggcgg catcctgcag ggcatgcggc tgccgctcta
     8401 cgccatgggt gtcgccctct tccgccacgt cttcggcccc ctggtcgtgc tctatctgct
     8461 cgacccggtg atgggcttcg gcctgcccgg catctactgg ggcatcgtca tcatcgcctg
     8521 gatcgggtcc gcggtgacgc tcttctatct gcgccgcgag ttgcccgacc tgccagcggc
     8581 ggagagtttg acccgctgaa tgatcgaccc gtcgcctcgc ggcaggaacg gccacaatca
     8641 gacttgtctt cgatccggtc gaagcgcatc ctgaccgaaa cgctcggcat cacattccga
     8701 gcagagtgaa cgcatcggtg aagaacccag gaggatcggc tgatgttcgg aaagttcacg
     8761 ttcgcgagca aggccgacta tgcaacgctg gactgggggc gggtcggcgc gctttgcagt
     8821 ccgtcgggca ctggcgccaa gcaacttacc attctcgagg gccgactgta cccgaacaag
     8881 ggccatgact ttcataagca cgtgaatcaa gaagaagtca tcttcgtcgt ctccggccaa
     8941 atagagcagt ggatcgacaa cgagaaacaa attctgggcc cgggtgattc ggccttcatc
     9001 ccgcccgaca cggtccacgc ttcgttcaat gcaggggacg gcgaagccaa tattctggcg
     9061 atcttcgggc catccctcgg cgagaacggg ttcgagatgg tcgacatggc gacggaggcg
     9121 ccgtggaaca gtctgcggcc gaaatcgtga acgggtaggg ctcccggcct taccgggcgg
     9181 gcacggcgca ggcggacctc cgcgtgaccg gcccggcggc gggcgcccgt gctcaggcgg
     9241 cgaagcgctg gccggccctg cggatcgcct cggcctcggc gcgggacttg tgcacatagc
     9301 ggcggtcgca gtatttgcag accgtctggc cgcccttctc gagggtcaga tactcgatgg
     9361 ggtggccgag cgcgccgttg ccgccgtcgc actggacgtg catccggtcg gtgaggacga
     9421 cctcctcgcc gtcgaccaga tagacttcct cgtcagccat gccttcgctc tcccggcgac
     9481 ctcgccggcc gcagccagcc ggagccgctc catgaacgcc cgcgccgcat tcgaaagctc
     9541 gacgaccggg gccgccgctc ccttgccagc ttatgcgatc gagctcaagg gcctgcgcaa
     9601 ggtctaccgg gcgagcggcg gccagccgga caaggaggcg ctcaagggca tcgacctcca
     9661 ggtgccgcgc ggggccatct tcggcctgct cggcccgaac ggggcgggca agtcgacgct
     9721 gatcaacatc ctggccgggc tggtcgtgaa gacgggcggc tcggccgagg tcttcggcat
     9781 cgacatcgac cggcacccca ggcgggcacg cctcgcgatc ggcgtggtgc cgcaggagct
     9841 caacctcgat gccttcttca gcccgcgcga ggtgctggag ctgcaggccg gcctctatgg
     9901 cgtgccgaag cgtgcgcgca ccaccgacgc gatcctcgcg gccgtcggcc tcgccgacaa
     9961 ggcggacgcc tatgcgcgct cgctctcggg cggcatgcgg cggcgcctcc tggtcgccaa
    10021 ggcgctggtc caccatccgc cggtggtgat cctcgacgag ccgaccgccg gcgtcgacgt
    10081 cgagctgcgc cgccagctct gggcgcacat gcgcgagctc aatgcccggg gcatcaccat
    10141 cgtgctgacg acgcactacc tcgaggaggc cgaggcgatg tgcgacagca tcgccatcat
    10201 cgaccagggg cgcgtcgtcg cctgcgacac gaccgcgacc ctgctccgcc agctcgacga
    10261 gaagatgctg accctgaccc tcgaccagcc gctggcccgg ctgcccgagc ggctggccgc
    10321 cctcggcgcc gagctcgacg aggccggccg gatcgtcgtc cgctaccggc cgagccgctc
    10381 cagggtcggc gagatcctgg aggcggcgag cgccgccggg ctggcgatca gcgatctcgc
    10441 cacggccgag gccgacctcg aggagctgtt cctcaggctc accggcccgg ccgccgcccg
    10501 ggacgagcac cacgacgctt tgccacgggc gcggagtgcg ctataggcag ccggtcagcg
    10561 cccgtagctc agttggatag agcgcgtgcc tccggagcac gaggtcagag gttcgagtcc
    10621 tctcgggcgc gccacatttc ccgatatgcc gcattgctcc cagctgcgca tgcgaattgc
    10681 gccggatcag gcccggctcg ccggatcggc gaacggctcg tcgggctcgc agacgtagaa
    10741 gagcgtcatg cgcgccggca cgttgccctg gttgctgccg gtcatgttga cgccggacgg
    10801 ctcgacgaag atctcgccgg cctcgatgct gaccgtgccg cgatcgtcca gctcgagggt
    10861 gaacgtgccc tccagcatgt agacggtcac cgggtgccgg tgcgaatgat acggcgtgac
    10921 atcgcccggc agcagggtgg cggagagcac gcggatctcc tgatcctgcc tcctcggcat
    10981 gccgcggacc acctcgcggt ggagcggtgt cagcctggcc aggccctggt cctgcgcgct
    11041 tcgcgtgacg ccgacgtcca gcgctgcgat catggtgccc aagtcgctct tctggtgcat
    11101 ggtcgactcc cttgcattgg cagggagagg ctagcccctt cgctgctccg gcggcagcag
    11161 cgggtctgca agaccgccgc gcaatagtgc ataatgcgcg ccatgttcga ctggaacgac
    11221 ctgcgccatc tgatcgcggt ctcgcgcgcc ggcagcacgc tcgccgcggc gagggcgctg
    11281 ggcgtcaacc aatccacggt gcaccgccgg ctggtggagc tggagcggcg catcgggctg
    11341 ggtctggtga agcgtcggcc ttctggatac cgtttgtccg agctcggcga ggcgctgatc
    11401 gacgatctgc tggccgtcga gaccgcggtc ggtgatctcg aacggaagat aaggtcgctg
    11461 aagctcgacc tcgcaggaac gattcgcctg acctgccccg agccgatcgt gccgcggatc
    11521 gcggcgtccg gtctgctcga tcgctttcac gagcgctatc cgggcctcct ggtggagctc
    11581 gtgaccagcg atcgctacct gaacctggcc gagggcgagg cggacatcgc ctttcgctcc
    11641 ggcgagccgg tggacgaccg gctggtggga cggaaggtat gcgactccgt ctgggcggtg
    11701 tatgccagcc ggagctatgt ccaaaagcac ggccggccaa gggacatcgc cgatctcgcc
    11761 acgcacgcgc tgatcggctt cgacggcatc atgcagggcc atcgggtcgc caggtggctg
    11821 ccggcggtgg ttcccgacgc ccggatcgtc agccgcacct ccagcatgct cggtgccgta
    11881 gccgcggtga aggccggggt cggcatcgcc ccgctgccga ccacgctggg cgatgcggag
    11941 gacacgctgg tccaggtgct gccgccggtc gaggcgctga cccgtgcctg gtaccttttg
    12001 acacctgccg acctgcgccg gacgccgcgc atcgccgcct tcgtcgattg gctgctcgac
    12061 gacattccgg cgctgcggga ggcgctgatc ggctgacccc cgctggaagc gggattcacg
    12121 gcgacgacac cctgccttcc gggccggggt cggcgccgcg ccgaaggccg gcggcccgcc
    12181 cgtgccggaa tgcggcgggc ggtcaggcgc ggtgccggcg cagatgcgcc caggcgcgat
    12241 cggccccggc caggatctcg atcagtcggg cgacccgcac gtgaacgacg cgctcggtga
    12301 gatgcccggc gctctccacc agggcctcga cggcctccct cgtcgcccgc tcgatcgtct
    12361 cgccacgcgt cagccggccg gcggcgcggc cgagcttcgc cactgcgcgc gccgccgtct
    12421 cgcgttcccg cacgccggcg agcacggtcg gggcgtcccc cgccacgacc ggtgacatgg
    12481 ccaggtcctg gatggcggcg agatagcggc tctcggccat ggcgccggcg cgccggccaa
    12541 gcgccgttgc ggccccggcc tcgtcgacga cggcctcggc gacccgctcg acggcctcgg
    12601 cgacttgcgc caccggtgct tcgggccggg cgacgacgcc gtccaggagc gaatccaggg
    12661 tggcccgatg ctcggcgaga attcggtcga tcaatggccg caggcgatcc tggcccgcgg
    12721 gctcgacggc ggcgaggaga tcgtcgacca gcgccgccgg ccgccgggtc gaccggagca
    12781 gcagcgtcag gagcgtcccg caccggtcgt cggagccggc gagcgccagg gccaggatct
    12841 cgccgagcac gcccttgtcg tcgtcatcga ggagctgcag cggccggggc agccggccga
    12901 gcaggggcac gaggtcgggg gcgatggcga gcaggtcggc gacccgccgc tgctcgacgg
    12961 cgagccggtc gggcagcccg gcgaccgccg ctgccgcggc cagggcggcc gcggcagcgg
    13021 gccagcaggt gcggccatgc tccaggatcg cgtcggtctc gtgcgccagc cgcccggcga
    13081 gcgctgtctc cagcgccgcc gccagcccgg gcggcagccc cgccaggccc gcctcgtgca
    13141 gctcgcccag cctggaccga tcgacgacga aggaccaggc gccgtccccg gcggtggcga
    13201 gcgccccctc gagcggcacg gtgagcaggc gctgcagggt cagcgggcgc tcgggccgga
    13261 gccggcgcag gcgcgggcgg agggccgcca ggatcggctc gacggcgccc cgctcgcggg
    13321 tcgattcgag catgcgcacg acatcccgga gcttctggtc gtcgaccagc tggaggcggt
    13381 cggcgaggcg gtcgtcaccc ttgttcgtca tgatcaaaga gcctccgcca ggacctgctc
    13441 gatcaccgcc tggcggtcga gcacatggcc cgtgcgccag tcctcgttga ggcgcaccca
    13501 gcgcctgagc tcgggattgc cgcggacctc catgggcttg ccgagccact cgtcggcggc
    13561 gagcggcacc aagggggcga gttcggcgag ctcgaccagg atgtcgcgct gcgcctcgcg
    13621 gggctcctcg gccccgccgc gccagagtgc tgccagatgg cgccagccgt cgagcagcca
    13681 gatcggccgg gtcaggacct cgtcgacctt ctgccggtcg ctcgcccgcg catgcagcag
    13741 gcggcgggcg ctgttcgcga tggtcttcgc cttggcgagg cccggcccgg tgcagtcgat
    13801 ggtcagggtc gcgcattcga cgacgaggcc ggcgaggatg gcatggtcgt cgcgctcgcc
    13861 ccgcacccac tcggcaatcg agcgccggag ctcgaccacc tcctcgagcg tcgtgtcgga
    13921 caggctcttg aagggggctt tcgtgtgccc gatcgaggcg accagcccgc tcagctcgtc
    13981 gagcatggtg atcagctcgc ccgaggcgat gcccatgctg ggctcgaggc tcttgaagta
    14041 cgggcgcaat cgctcgcggg cgtcgctcga gcccgtgtcg atgttcttcc agtcgatctt
    14101 gccgagatcg gcctcctgca ggagctgctc gagcaggagc agcatggtga gcagcgagcg
    14161 ctcctcctcc tcctgcagcg agcgcgcggc agccgcctcg gcatcgggcc cggcgaggcc
    14221 ctcccgggac agggacaggg cgatctcgcg gatgccgagc gggctgaccg gcgtgaggcc
    14281 ctgcattcgc tcgaccagcg cccggtcgtg cagcgagagc cgatagagct cgttcagcgc
    14341 cgccaagggc accacataca tcccatcggc gccggtcggg ttgcgggcga cgatctcgag
    14401 ccggccccga taatccagcc gcatccgcgc ctggacaagc agcggcgtcg tgaacggcgc
    14461 cacgactccg gcctcggcga acgtcgacgg ccgctgcttg agggagaaga gacggcgtct
    14521 ggcggtcaag cgggcctcgc gcactggatg ggagcgcact ggataaggtg accaacctac
    14581 ccgcaatcaa cgaagatctg gttaacgccg ccggcttcgg gcgggcgccg gtgccttgac
    14641 aggtcggggt gccgggcggc aaggcaccgc tttcgccagc cgtccggatc gccgccatgc
    14701 cagcctcgcc gaaaggccgc gtgcccagca cgatcaccta tctcgagcgg ctcgacccgc
    14761 ccaggggagc ccccctgcag ccgccctgcc ccggcaccga gatcgtccgt gcgctgaacc
    14821 cgcccgtgca tttctaccgc tatctctatg cgacggtcgg cgccccctgg ctctggaacc
    14881 ggcgccggct gatggacgac gcgacgctgg cggcgatcat ccaggccccc tcgacggacc
    14941 tgcgcgtgct ctggctgcac ggcgtgccgg ccggctatgt cgagctggat ttcggcgagc
    15001 acccggatgt caggctcgcc tttctcgggc tcgtgccgga cttcctcggc cgtggcctcg
    15061 ggccctggct cctcgactgg gcgatccgcc acgccttcgc caagggcgcc acgcggctcc
    15121 atctcgacac ctgcgacctc gaccacccca aggcgctcgc cacctacgag gccttcggct
    15181 ttcaggccta cgaccgggcg aattccttcg agacgctgct cgacgacatg gagctgccgg
    15241 cgcattgccg cgacaagccg gtgctgccgc tcgactgaag gggattgcgt ccggcgaccg
    15301 atagcatcat atcagactgg ttggtccaac tggttggaat gatcatccca tgctgtcgat
    15361 cggcgctttc gcggccaaga cccatctttc cgctctcctc gagcgcgtgg cgcgcggcga
    15421 agaggtgctg atcactcgac atggcaaggc aattgcccgg ctggtgccgg ccgatacggt
    15481 ggcacccgtg gatcacgcgg cgctggtggc acggctcaag gcggcgcggg agaaggccga
    15541 gctcggcgac ctcgactggc gcgcgctgcg tgacgaagga cggcggtgag cctcgttctc
    15601 gactgctcgg ttgcactcgc ctggtgcttt cgggacgagg ccagcgaggc gggcgacgcc
    15661 ctgctcgacg atctgccgga gctcgccccg ctggttccgg ccctctggca tctcgagatt
    15721 ggcaacgtcc tgctgatggc cgaacgacga ggacggatca ccagcgccca gttgcaggag
    15781 catctcgccc tgctgcgcgg tctgccgctc gaaaccgacg acgaggcgcc ggcccgcacg
    15841 ctgccggctg tgctggcgct ggccggtggg caccggttga ccgcctatga tgccgcctat
    15901 ctggagctgg cggtgcggcg aggcgcatcg ctggcgacac gcgactctgc actggcgcgc
    15961 gcagcgagat cagtgggggt gaagttgcgg caagcctgag caaaatggag ggcatcgatc
    16021 ccctccgacc tcctcgcggg cgagccatgc ggcgtaggcg agagcggcgc ggatatcctc
    16081 ggccacgaga tagggatagt cggcgaggat ggcctccacc gtcagaccat cggcgacgag
    16141 gccgagaacg gtagcgacgg tgacgcgcgt gcccttgatg cagggccggc cgcccatgat
    16201 ggacggatca aacgagatgc ggcccatttg ctccggctgc gccaatgact cgaatccctt
    16261 gcaaggcttc gcccgatcgg cagtctagca catgtcgggt ggcatgccac acggcggcga
    16321 gcgaccgtca gtcctcgagc tcgatgacga ggccgtcatg ggcgggggcg acgccctctg
    16381 gcagcctggc ctgccaggtg gcgtagtcga cctcgtggtt catgtgggtg agccaggcct
    16441 gcttcggccg gacgcgggcg atccattcca gcgtgcggtg gagatgcgag tggctcgggt
    16501 gcggcttgtc gcggagcgcg tcgaccagcc agacgtcgat gccggccagc gcctccagat
    16561 aggcctcgtc gagccggtcg gcatcggtcg agtaggcgaa cttgccgatg cgaaagcccc
    16621 agacgcggct ccgcccgtgg ccgtgggcca gcggcaggag cagggccggg ccgatggtga
    16681 aggggccgtc gccggtgaac ggccggggct ggatctccgg ccgccagaag ccgccccggc
    16741 cgtcctggcc gccgccgaag gcgtagggga agcgctcctg gatgcgctgc aggacgtcgg
    16801 catgggcgtg ggcgggcagc gggcggccga tgacgttgtt gatcgcccgg atgtcgtcga
    16861 gcccgtgcag gtggtcggca tgggcgtggg tgaagacgag cgcgtcgagc cggctgatgc
    16921 cggcatcgag gcattgccgg cggaggtccg ggccggtgtc gaagaggatc ttctggcccc
    16981 gggtctcgac caggatcgag cagcgcaggc gcttgttccg ggggtcgggc gagcggcaca
    17041 cggcgcagtc gcagccgatc tgcggcacgc cggccgaggt gccgcagccg aggaccgtca
    17101 cccgcatcag gggcgcgctg ccttcttgaa gaggcgaaag aaattctcgg tggtgatgcg
    17161 cgcgacctcg tcggtgccgg tgccccagag ctcggccagg acggcggcga cgtgcgggat
    17221 gtaggccggc tcgttgcgct tgccgcgatg aggcaagggg gcgaggaagg gtgagtccgt
    17281 ctcgagcagg atgcggtccc tgggcaggtc tctcaccgtc gctcgaagct cgtccgagcg
    17341 cctgaaggtg aggatgccgc cgaggccgag atggaggccc atggcgaccg cctcgtggcc
    17401 catctggggc gaggtgctgt agcagtggat gacgccccgc agcggcgctg ccgctgctgc
    17461 ctcatcgagg acggcgagcg tgtcggcatc ggcgtcgcgg gtgtggacga tgaagggcag
    17521 gccggtcgcc ctggccgcct cgagctgggc cgcgaagctc gcccgctgca cctcgcgcgg
    17581 gctcttgtcg tagaagtagt cgaggccgca ttcgccgatc gccacgaccc tggggtcggc
    17641 ggcactggcg atgaacgggg ccgggtcgca gctttgcgcc tcctcggccg cctggtgcgg
    17701 gtggacgccc gaggcgaccc agacgttcgg aaagcggcgc gcgagctcga gcaccgccgg
    17761 caggctggtg agccgggtgg cgatggtgag cagcgtcgcc acgccggcct cattggcgcg
    17821 cgccaccacc gcctccacct cggcgacgag gcccggatag tcgaggtggc aatggctgtc
    17881 gacgaaggcc ggcaacgact caggcaccgg ccgcgtcctc gacgaggcgc gggaagacgc
    17941 ccgccggctt cggcagggcg gtgccggcga cgagcgcacc cctgtcgtcc agcgatgcga
    18001 aatcgcgcag ctccgggggc tgggcgagct ggtcgaggat gcgcgccgag gcgtcgggca
    18061 tgaagggctg ggtcaggatc gccacccggc ggatggtctc ggccagcacc cagagcaccg
    18121 tcgccatgcg ctccgggtcg ctcttgcgga gcgcccaggg tgcctgcgtg tcgacatagc
    18181 ggttggcgtc ggcgatcacc gcccagatgg cctcgagcgc cgcgtggctg gcctggcgcg
    18241 ccatcagctt tctgcaggtc tcgaggaggc ccgccgcctg gtcgagcagg gccgtgtcgg
    18301 cgggcaggat ggggccgggc cgcggcacgg cggcgccgca attcctcgcg atcatcgaca
    18361 gcacgcgctg gcagagattg ccgtagtcgt tggcgaggtc gctgttcatg cggcgcagca
    18421 tggcggcgtg gctgaaatca ccgtcattgc cgaacggcac ctcgcgcagg aggaagtagc
    18481 gggtggcgtc gaggccgtag cggtcgatca gcgcgtgcgg gtcgatgacg ttgccgaccg
    18541 acttgctgat cttctggccc tcgttggtcc accagccatg ggcgaagatg cggcgcggcg
    18601 gctcgaggcc ggcggccatc aggaaggccg gccagtagac ggcatggaag cgcaggatgt
    18661 ccttgccgac gacatgcacg tcgcagggcc agaagcgggc gtagagcccg tccgggtcct
    18721 cgggccagcc gagggcggag atgtagttgg tgagcgcgtc gagccagaca tacatgacgt
    18781 gcgccgagtc gttcggcacg ggcacgcccc aggcgaaggt ggtgcggctg accgagaggt
    18841 cgttgaggcc gcccttcacg aagctcctca cctcgttcat ccggctctcg ggcatgaccg
    18901 agtcgggctg ggcctcgtag taggcgagca gccggtcctg ccaggcggag aggcgaaaga
    18961 agtaggaggg ctcctcgacc cactcgaccg gggcaccggt cggcgccttg ccggcgacca
    19021 gctcgctctc ggcgtagaac gcctcgtcgc gcaccgagta ccagccggca taggcgccga
    19081 gatagatttc gccggcgtcg agcagcttcg tccagagctg ctgcacggcg tgcttgtgcc
    19141 gcgcctcggt ggtgcggatg aagtcgtcct ggctgaagcc catcacccgg gcgagctcgc
    19201 gaaagcgggt cgagacctcg tcggtgaagg cctgcgggtc catgccgcgg tcacgcgcgg
    19261 ccttctccac cttctggccg tgctcgtcgg tgccggtgag gaagcgcacg tcgcggccgt
    19321 cgagccgcat gaagcgggcg agcgcgtcgc aggcgagcgt cgtgtaggcg tgcccgatat
    19381 gcggcgaatc gttgacgtaa tagatcgggg tcgtgatgta gtacgcagca cgggccgcca
    19441 tcgtcgtctc cagccgggtg ccggcagcgg ctctcgatca gccggccagc cgctcccgcg
    19501 tggccccggc gagcgacgcc agtgccagat agagggtctg gcgcggatcg aggttcaggt
    19561 gatcgagctc gcccggcagg tcgcgcagct tgtcccacaa gcccatccag cgatcaagcc
    19621 gcctgccccc gaccagggcc gcgagcctcg acgcctcgcc cggcgccagc tccgggtcga
    19681 tagcacccct ggtcgcctgc tcggcggcgc ggcggaggat cagggcgagc agctcgacga
    19741 caagcccggc attgccgatg ccggcaaggc ggtcggcggc ctccaccagc gggcgccggt
    19801 cgtcccgccc ggcgatcagg gcatcgagca gggctgcata gtgctcgaga tagccggcgg
    19861 caccgagcat ggcatagcgg ccgggggcac ccttcgccag cgccaggagc gccggcgagg
    19921 cttcgcccgg cgcggccagc ccggcggcgg ccagggcctt ggccatgggc tcgggggcga
    19981 ggggctggag gcggaggcgg gcgcagcgcg agaggatcgt gcgcggcacg cggccgggtg
    20041 cgtggcagac gagcaggagc acgacgccgg cgggtggctc ctcgatcagc ttgagcagcg
    20101 cattggcggt gttgcggttc agatcgtcgg ccgcgtcgac gacgatcacc cggccatggc
    20161 ggccgaccgc cgtcgcgtgc agcgcggtcg tgaggtcgcg cgcctgcccg acgagaatca
    20221 cgccgctcat ccgattactt cttgggttga cctcgttggc gagggcaaag agatcaggct
    20281 cggaaccatg cgcgatccgg cggaaaatag cgctttcggg gtcgttgggg tcgaccatgc
    20341 cggggccggc gaggatgcgg cgggcgaggc ggtaggcgag cgtcgccttg ccgatgccgg
    20401 ggggaccctg caggagccag gcgtgcggca gccggccgct gcgccaggcc cgctcgaaga
    20461 cctcctccgc cgccgcgtgg ccgaagaggt gcgggttcgc gcgcggtgcc ggtggtccct
    20521 gctcgctcat ccggcgagcc ggtcggcgaa gcgggcgagg atggtggcct cgatccggcc
    20581 ggcgaccgcc tcggccggct cggtcgcatc gatgacgacg aagcggccgg gctcggcgcc
    20641 ggcgagctcg aggaagccgg cccggacccg ttcgtggaag gccgtgtcca gcgcctcgaa
    20701 gcggctgccg ccgccggctt tggcgcggcg ggcgaggccg gcgctcaccg gcaggtcgag
    20761 gaggagcgtc agatccgggc gggcccctgg caggaagagg tcgtgcaggt cgtcgatgcg
    20821 cttcgtgccg agccccccgg cgatgccctg atagacccgc gtgctgtcgc tgaagcggtc
    20881 cgagagcacc cagcttccgg cggcgagggc gggctcgacc agccgctcga catggtcgag
    20941 ccgggccgcg gtcatcaaga gcgcctcgac gccgtggctc cagcgctcgg ccgggccctt
    21001 caggacgagg tcgcggatcg cctcggcgcc gggcgtgccg cccggctcgc gcgtggcggt
    21061 gacagtggcg ccgcgcgctt ccaggaggtg gcgcagggcc tggagctggg tggtcttgcc
    21121 ggccccctcg ccgccctcga aggtgatgaa gacgccgcgc gcgcccgggg tcgcgctcag
    21181 cccggcactc cccagatgag gtattcgagc gtgccggtca tgcggccgag gatgccggcc
    21241 cgctcgaccg gccgggcggc caccagcggc agcacccggg tcggcatgcc gtcggcggtc
    21301 atctcgatct ggccgaccac ctggccggcc tcgacgggtg cggtgaccgg gctgtcgtag
    21361 accaccttga cgttgagcga gtcgcgcgcg gcgcgggtca gggtgatgct caccacatcg
    21421 gcgccgacga gcggcacggt cggctccgcc ccctgccaga ccggcgcctc ggcgaccacc
    21481 gcgtcgggct cgaagaggcg gtactcctgg aaatggcgaa agccgtactc cagcaggcgc
    21541 tcgctctcgc tgcgccgctg gcgcgaggat tccaggccgg ccaccacgag gatgagacgc
    21601 cggccatcgc gctccgccga ggcgacgagg ccatagccgg cctcctgcgt gtagccggtc
    21661 ttcatcccgt cgacacccgg gatcccgccc tgcagcagcg ggttgcggtt gtgctggttg
    21721 atgccgttga actcgaactc gagctgcgag tagtactggt agtactgcgg gaagcggctg
    21781 atgatctggc gtgcgacgat cgagaggtcg cgcacgctca tcagatgctc ggggtcgggc
    21841 cagccggtgg cgttcctgag cgtcgtgttg gtcaggccga tctcgatgcc gcgcttggtg
    21901 agccggtcgg cgaacgcctc ctcgctgccg gagagcgcct cggcgatgac cacgcaggca
    21961 tcgttgccgg actgcacgat gatgccctgc aggagctcgt ggacggtggc ccgggtgccg
    22021 acctcgagga acatgcgcga gccgcccatg cgccaggcgc gctcgctcac cggcagttcc
    22081 tcgtcgagct gcaggttgcc ggcctcgagc tcctcgaagg cgaccagcgc cgtcatcagc
    22141 ttgctcatcg aggccggcgg cagcgcctcg tccgggttct tctcgaagag gacgtcgccg
    22201 ctgcggtagt cgatcaggat cgccgcccgg gccgccgtct cgaacgcggt ctgcgcccgc
    22261 gcggggccgg ccgcgatggc cgtggcgagc aggacgagtg ccgcaagaag ccgcatcatg
    22321 gtgttggatt tcccttcgat tcgacccggc cgaccgggcg ggtcaagagc agctgacgac
    22381 gagcgcatcg cgatagccca tgccgtgcac ccgggcgagc gtgccgaagg cgtcgctgcg
    22441 cgaggcatag gggccgagcc ggacccggct gacggggggc gccgtctgga cgtcgaccgg
    22501 gccgaggccg tggagctgcc gctcgagtgc cgccgcgttg gccggctcgc tgaaggcggc
    22561 cacctgcacg aaatggtcat cggcaccgca gcccgacgcc ctggcggggg ggcgaacggg
    22621 cgaacgggtg ggcgccctgg cgggcgtcga gcggatgacg ggggccgccg cggggacggc
    22681 gctcgccacc acggtggccg ggcgggtggt ggtgccggcc accggcggca cgccgtgggc
    22741 cggcaggagc gagacgaagc ggacccgcac ccgggcgagg cccttgccct cgaagccgag
    22801 ctccctggcg gcggcccggg agagatcgat caggcggttg tcgtggaagg gcccgcggtc
    22861 gttgacgcgc agccgggcct tgcggccatt gtcgaggttg gtcacctcga cgacgctcgg
    22921 caagggcagg gtcggatggg cggcgctcgg ctggcgcatg tcgaagacct cgccattggc
    22981 ggtgggcagg ccatggaagt cgtcgccgta ccaggacgcg atgccggtct cgtcataggc
    23041 ggggtcgaac tcggggaagt accagatgcc gttgatccgg tagggctggc cgagcttgta
    23101 ggtgccgtgc ggggtcgtgg cgagcggatc gaggtcgggc ggagtcgacc tcggccccga
    23161 gcaggcggcg agcagcagcg ccgcgagcgc cagggcgagg aacgggcccg tgcggcggcc
    23221 ggtcatgcgc ggatggcgtc ggcgagcagg ccgacgctgg tggcgaaata ggtggaccgg
    23281 ttccagcgca tcagcacccg gaaattgtcg tagacgaggt agctcgggcc gctgccgtcg
    23341 tccatcttca ggagcgaggc gtcgatcgcc acggccggga gcgcgctgcc atccagcttg
    23401 cgcacgcccc tggcctgcca ggtggcgagc gggcgctcct cgtcgagccc ggccgaggcg
    23461 acgccgctgc cggccttcac ctcgcgaccc cagatatact cggggttcca gcccatcttg
    23521 ctgagataat tggccatcga ggcgaagatg tcggcgtgcg tcccccagat gtcgcgccgg
    23581 ccgtcgccat tgccgtcgac ggcgtagccc agataggtcg acggcatgaa ctgcgactgg
    23641 cccatcgccc cggcccagga gccgaacatg gcgtcgggct cgatgtcgcc ggcctcgagg
    23701 atctggagcg cactcaagag ctcgccgcga aagaagtcgg cgcggcggcc gtcatgggcc
    23761 agcgtcgcca gcgaggcgac caccgggaag cgccccttga actcgccgta gctcgattcg
    23821 atgccccaga gggccacgat gatctcggcc ggcacgccgt accgggcctc gacctgccgg
    23881 agcatcgggc cgtgctcggc gagcagcctc cggccctggc tgatccgtgc ctcggagatc
    23941 accttgccct tgtactcgac gaagctcatg cggccttccg gctgccgctg gtcgagctcg
    24001 atcacccgcg ggatgggctc gaccccggcc agcgcctcgt cgagggtgcg gtcgctgacg
    24061 cccctggcgc gtgcctcgct gcgcaagccg tcgagccagg ccgcgaactc ggtcgcggcc
    24121 atggccggcg cggcgaggcc gagcccgaga accaacgccg acgcgagccg ggcaagacgc
    24181 cggcccccgc ccatcatgat cgggcatgat cgcaacgcca tcgagcgccg agcctccatc
    24241 aggtttccct acctgaggtt atgctcggcg ccgacagctc gatcaagtcg cccggcgtgc
    24301 tcagtacgtg aaagtgacga agcgaacacg tttgagcgag ctcgcggcaa ggcggctggc
    24361 cggccactcg atcctgggca agggcggcag cggcgggagg tcgaaggtta tcttcgggat
    24421 ggtggcgatc ggcgggatgg cgccggggcc gccgcagaac tgcagcggcc gaccgagccg
    24481 gttgtcgata cggatctggc aggactcgac gacccgcacc ctgatctgca tcgtggtgga
    24541 cttgcctgcg gcgttcgccg gcgccgacgc gccgaccgtg gcaaggccca gggccgcgac
    24601 cgccgcgacc ttgagccgac ctcgagctac ctgctcgacc atggggctca ctcccgttca
    24661 tcggccgcct acgtgcgacc ggaggagcgc agtcatagca caacgggcgc atgcgtgtgt
    24721 tagcggccgt caaccatggc gagccccgga tcgccatcgc tcagaacgtg accgacacgg
    24781 tgagcgagtc gagatactgc ccggccggca cggctttctg gatggggacc cggccgtaga
    24841 tcgaaagcct cgtcgtctcg tcgccgttgc cgctcgccgc cacgacggca ccggaaccgc
    24901 tgccgtcggt ccagggcacc gagcgggtgg cgtcgggata gacgtcgtag gtgagcctcg
    24961 cgccatcggg ccccgagagg tagcggttgc ccgggctgcc attgccgacg atggccacct
    25021 cgaagctggt ggccgtctgg caggagacgg cgatctcgcc ggtgctgtcg gcgccgcggg
    25081 cgacgtcgac gacgccgaag gcgagcgcgg tcgtggcgaa ctggcagacg gcgagcgccg
    25141 gccgggcggc gatggcgcag agcagggcgg cgcagcacca gatgcggatc agtcggcgca
    25201 acggatctct ccgaggtcgg gcaagggatc ggtgccggcg aaggccggca cggcgcagac
    25261 gaagcggcgg cccccctgct cgccgatcag ataggccttg gcgtggagcg ggccggtgag
    25321 ctgggtgaag ccgtcgcggg cgacgaggcc ggagagcccg cccgtttcgt cggcgaggac
    25381 gagcccgggc ggcagcggcc ggtcggcgcg atcgcgaagg gtggcggtgg cgcgctgcac
    25441 ggcgacgatg ccgaaatcga tggtcatgcc actgcggtcg aagggcaccg ccgccgtctc
    25501 ggcgatggtg agctcggcgt cgatgggcag gtcctcgacg gcgaggctga tccggttccg
    25561 ctcgtagggg ctgagatcgg gcaggagcag gcggccgtcg gcgtcggtcc gaccggcctc
    25621 gcggttgtcg acatagacgc gcacgtcggg gaagcccggc aggtcgacga ggccgaaggc
    25681 gcggccgatc cggcgggtga ggccgaaatt gccgtcgacg agggcgaggc tgccgtcggc
    25741 gccgaggcgc agccggttgt cgccggagaa gcgttcgtag gcgacctcgg tgccgatctt
    25801 cgatgcctgg tagccgagcc aggcgtcgac cggccgcgca tcgtcgccga tctcgctggc
    25861 caggcgatag tcgaggccga gctcggaggc gccgcgggtg cgggtgtact gcagccgccc
    25921 gcggttgcgg ccgtcgccgg catcggcgat cgcggtcatg ctgcggttcg agccgagcgg
    25981 gatcgagtag gcggcggtga gcgcgaagtc gctgtccggc gacagcgtct gggcggcgtt
    26041 gacgaccaag gcacccggcc cgatcggcag gctgtagctg gccgcgagcg actggtcgtc
    26101 gtcgcgattg atgcggtcgc ggttgagaaa gaggagaccg agccggccga tgctgccggt
    26161 gtcgtagccg atattgagct ggtcgacccg gcgggtgcgg ccgtcgtcgc tcgaggcctg
    26221 gcggaaatcg tcggaggtga agcgggtccg ggcccccagg ctcagtcgcc tgccgacata
    26281 ctcgtaggcg agctggccga gccagccctc gtcggcaccg ctgtcgacgc tgccgccgag
    26341 cccgccggag agcaggccat aaggtccgag cagcagggcg ccgccgccga ccaggctggc
    26401 gccatcctgc cccgcctcga gatgggcctc gccggtgacg gtgtcggtga agccgtagcg
    26461 atgggtggcg gtccccagcg cgtcgccata ggcgaagctg cgctcgccga agtcgttgcg
    26521 cttgaagccg gcctcgtagg agaagtcgtg cagcccggcc ttcagcaggc gggtgctgac
    26581 atagtaggac tgggtgacca gccgctcgcg gccgagcagg tcggtgacgc ggagctggac
    26641 ctcgccggcc ccggtgacga ccggcacgtt gtcgatggcg aacggcccgg gtggcacggt
    26701 gcgggtgatg ctggtgaggt tgtcgacgat gacgtcgacc accgactgct gctcggcgag
    26761 gccgccgatg gacggcaggg ggaaggtgac gaaggcgggg tcggtggcga agttggtcga
    26821 ccactgcaac ccgccgaagc gcaccggctg ggcgaaggcg ccgccgacgg tcaggctgtc
    26881 accggcgcgg aaggtggcgc ggtgctcggg gaagtcgtgg aagaaggtgg tctcgaggcg
    26941 gtcgatctcg gcctcgtcgc ccgcgtcctc gacgcggaag ttgttgagga agacattgcc
    27001 gtggctgaag gcgcccgcct cgaacagccc gtcgagctgg tcgtcgatgt cgccgccgcc
    27061 ctggagcagg aggtcgtagt cgaaataggc gccggtgccg ggcgtcggcg gcgtgcgctc
    27121 cagcccctcg aggtccatgg cgaagaggcc gaagacgtcc ggcggcgcct cgatggcgac
    27181 gaccagtgcc gccggatcga gcaccgccac ggcgcccggg atggcatcca ggggatagaa
    27241 cggcacgcct tcgaaggtga ggatcgcatc gcggtcgatg atcagccgcc aggcctcggc
    27301 ggtggcgagc tcgaccgcga gccggccgct caccgggtcc tcgatgaaga tcacgccgtc
    27361 gctgaccggc tcgccattga gctcgaccga cagcacccat tccgtccagc gctcggattc
    27421 gagcagcggc aaggcgagcg ggtcgcggcg gtaggtgatg ccgccgggct cgatggcgtc
    27481 gagcgcctcg agctgctcgg gcaggggcgc cgccggcggg tcggcggcag cgaccagcac
    27541 cggtggcgcc ggcttggggg ccggtcgggg cgccggcctg gggggcatcg cccgtgccgc
    27601 cggtcgtgcg gtgggtggca gcgccggcat gcccgaggga gcgtggttct gcaggatgag
    27661 gctggtcaac gccacggcgt agcgggccgt gtccggcggg ccctcggccg aggtcgccct
    27721 ggccggtgcc gtggccccgc ccggcccggc gagcggcgtg cggtcgacgc cgagcaggac
    27781 cagcaggagg ccgatggtgg cgctgccggt gaggccacga tgcaggcggc cgggcacgtc
    27841 agttcggcag ctcgaccacg aggtcgccga gattggtcgc cgcttcgacc accaggtcgc
    27901 gtcggccctg caaggacggc agtggccagc gctgcgtgtt gccggccagg acgtagctcg
    27961 gcgtatcgat cagggcgagc gggtcgccgc cggccgcggc gcgcaggctc aggcggctga
    28021 catggagatg ggcggtgccg gcgttgcgca cctcgagcgc cggcccgtcg gcaccctgca
    28081 ccagtcgcca gtccggcacg ggcgcggcac ccggcggggt gatgaagacg ggcaggctga
    28141 ggcgcagcgc gaagcggacg ccggccgcgg ccggatcgcg ggcgaccggc acctcggtga
    28201 tcaccagccg gtaggccgtc tcgacctcgg tcgaggcggg ctcgcgcgtg gcgacgcgga
    28261 tcacctgccg ggcaccccga tcgaggttga agaccgccgg cacggcgagc aggccgcggg
    28321 tcggctcgag ctccgccacc gaattgccgt ggtgccaggc gaaggtctcg acctgcaccg
    28381 tggtggcggc ctcgccggtg ttgttcagcg tgaccgagcc ggtgcgggag gcggggccga
    28441 ggtcgatgcg cgtcggcgcc acgttcagcg aggtggcgat ggccggccac ggcaccatga
    28501 cgaaaccgat gaccagagcg agcaccgctc gtcggtgcgc acgacgcatg atgcctctca
    28561 gaaggtcagg gtgatgttga cgatgtcgac ataggcgccg ccgggcacgg cctggccgcc
    28621 cggaatccgg ccgtagacga cggcctcgcc actctgcggc tggaggagct ggagctgcag
    28681 cgcgttctgg ccctgccccc agacggcgtt gcgcaccgag ttgcggtaga tctggtaggt
    28741 cagcgtgttg ccgctggagc gcatggctcg gttgttgaca ttgccgctct gcccgccgtc
    28801 cagctcgaag gtcaggaggc cggagcagtt ggcgaagccg atgcgcccgt tggcatcgag
    28861 attgctcgtc tggcccgaga cgtaattgcc gaagtcgagc gtgccgccgg tcagcgtgca
    28921 gccggactgg acggtggcgg tgacggtgag ctgatccgtc gcggtggcgg catgggcccc
    28981 attcggggct gccaccgggg aagcggcgag cgccaacgcg gccatggccc gggcgcacgg
    29041 gtggcgtcgc cgatggtggc gcgtggccat ggggtcgtct ccgggtcggg gtgcctggtg
    29101 gttatcaacc attacgcgat tatatgcgcc attaatcgct tatcaagaga tgaaatgaac
    29161 gacttcacac ctcaaggttg attggctatc aaggattttc gccattcggg cgtatccggg
    29221 ccggcgggcc ggtccggcca gcgggagacg cgtgatggac aagcccttgc ggatcacctt
    29281 cgacaacctg ccccacagcg aggcgatcga ggcgaacgtg cgcagccgca tggcccggct
    29341 cgacgccctc tgcgaccgga tgaccggtgc ccgggtggtg gtcggcagcc cgcatcgcag
    29401 cctgaccaag ggcaagacct atgcggtgcg gatcgagctg gcgctgcccg gccgcgacct
    29461 cgtggtcagt cgcgagccgg tcggcgacct gcacgaggcg atggccgccg ccttcgccac
    29521 ggcgcggcgc cgcctgcgcg gcttcgtcga gcgcagccgc ggggcctgag cgccaatccc
    29581 tgcgtcgacg cttggtcagg cagccggtgc cgggcgggcc aggcgctcga ccgtggcgag
    29641 cagggtggcg cggtcgatgg gcttggtgac gtagccgttc atcccggcgg cgaagcagtc
    29701 gtcgtcgtcg ccgcgcatgg cgttggcggt gatggcgatg atcggcaggc cggcagcgcc
    29761 ggggtcgggc agggcacgga tgcggcgggt cgcctcgagc ccgcccatga ccggcatctg
    29821 cacatccatc agcacggcgt cgaacgggat ctcggtgacc gcgtcgagcg cctcctggcc
    29881 gttggtcacg atctgcacct cgtggccggc cttcgagagc atgatcgccg ccagcttgca
    29941 gttgaccggg ttgtcgtcgg cgatcaggag gcgcagcttc tggctgcgtg cctcgccgag
    30001 cgcgtggacg gtgagcagct cgccctggcc ggcgctgccg tgcagctgct gcaggcagtc
    30061 gagcagcgtg ctggcggtca ccggcttctg cagataggcg tcgaagccga gcgcctggac
    30121 cttcgccgcg tcgccgcgca cgccggccga gaccagcagg atgagcttga gcccggcctg
    30181 gccgggtgcg gcgcggagga tggggcccag cgaatcgccg cggcggtccg gcagggtgcg
    30241 gtcgatgacg gcgaaatcga aggcggcacc gcgatcggcg ctctcctcga gcacggcctg
    30301 ggcctcggca gcgctcccgg tgaccctgac ctcgaggccc cagagccgcg ccagctcggc
    30361 cataatccgg gcggtgcgcg gctgcgcgtc ggcgatcagc aggcgcgcgc cggcgatggc
    30421 ggcgcgatcc cgacgcggtg ctgtggcgag cggcggctcg agctcgagat ggatctcgaa
    30481 ggtggtgccg cggccgaccg gcggggcgat gacgatccgg ccgtgcatgg tcgccgcgag
    30541 ccgctgcgcg atcatcaggc cgaggccgct gccgccgaac aggcgggcag tttgtgcgtc
    30601 ggcctgggcg aagggcgaga agaaatcggc gcggcggtcc tcagggatgc cgatgccggt
    30661 gtcgctgacg gcgatggcga ggccgaggcg ccccgccgcc gcgtccgccg gcgccacggc
    30721 gaggcgcacc tcgccctcgc tggtgaactt gatgccgttg gtcacgatgt tggtgaggag
    30781 ctggcggagc cggcccggat cgccgcgcac gacctccggc aggtcggggg cgagctggat
    30841 cgcgaaggcg atgcccttct gctcggcacg ctggcggaag accagggcga ggcgctcgag
    30901 gaacgggcgg atggcgaagt cgacgagggt gaaggtcgag cggccggcgt cgatccgggc
    30961 gagatcgagg atgtcgttga ccagggtgat gagcgactcg gccgagccga tgaccgcctc
    31021 gacatagccg gcctgctcgg cgtcgagcgc cgtgtcgcgc agcagccggg ccatgccgag
    31081 cacgccgttc atcggctcgc gcagctcgtg gctcatgatg gcgaggaaga ggccgcgctc
    31141 ctcgagccgg cgctgggccg cggcgagctc ggcgcgcaag cgctcgctct cggcggcgag
    31201 atcggggtcg gtcggggtcg gggtggcggg gcggctatcg gacatggggg ctccgtacgg
    31261 cacccacatt tctctaccca cggttgataa agaccgcaag ggagttgctc ttaggagatt
    31321 attcccctat attgcgcatg ggagcgccct ccggggctgc tggcggccgg aggcggagcg
    31381 gggcatggca atcgactggt gcgaagcggc gcgactcgtg gcagccggcc tgccgagccg
    31441 cgagatcatc gcgcgggtgg gctgctcgcg cagccagctc caccgacgcc ggacgagctg
    31501 cagcctgttc aaggcgctgg tggcggcgca tgcggccggc accgcggccg accgtccccc
    31561 ggaggatgcc gcatcgatcg aggcgaaggt gcgccggaag gtcgaggacg aggtcgccga
    31621 gggcaatctg aaggtcgccc tgtggctggc cgagcggttg cggatgttca cgcccgacgg
    31681 cgaggacagc ccggaggaca cgatccagcg cctgatggaa tcgatgacgc ctttggagcg
    31741 cgccgccttc tcgcgggcgg agtaggcggc ggctcaggta gcggcggcgt gcaggtcgtc
    31801 gagcaccacg aggtgcagcg agtcctgata atagagccgc gtgccgtcgt cgaaggcgac
    31861 gaagcgaaag cccgtgacat cgtcacctcc ggcggtgacg tgctggccga ccgcccccgt
    31921 gtcgatcggc tggctcaggg cccgcaccgc gagtgcgggg agatgcgaat cgttgaagag
    31981 cacgatctcg ccgttcgagt cctgcacaag atcggtcagc tcgagggtcg gaacgtcgtt
    32041 ctccggcgcc gacgccgcct ggtccgggac ggcactgggc tcgaggcccc gcgccgcgct
    32101 tgctgtcttg ctcatttttt ctagccgttg ttgcgatccg tacggccgat cgcggctcgt
    32161 tggctgcgat catgtcgaga aaaaaattaa ccatgtcggc gggccgcaac aaggctgatc
    32221 acctcgcggt cgaatgcacc cgagtaccga ttagtggaat aggcgattgg cggcccgtca
    32281 gccgcccctg gcgacttgct cgacccaggc cggatgggcg aacgccgcga cgtgcacctc
    32341 gggcgtgtag tatcgcaacc cctccggaat gggccgggcg cggagcgtgt cgctgcgcac
    32401 ccggcgccgg gtcgcgtcgt cggagcccca gcccagcgcc atgaagccgc cgacataggt
    32461 gggcaccacg gtgagcagga agtcgacgtc ggcgaagccg gccgcccgct tgcgcgccat
    32521 gacgtcggcg agctccctgc cggccagggc cgggttgccg gcctgggtca cgaggatgcc
    32581 gccctcgttc agccgtcggc ggcagtcgcg atagaagctc tcggtgaaga gggcggcacc
    32641 cgggccgatc gggtcggtcg aatcgaccag gatcacgtcg aagctctcgc cgtctttggc
    32701 catgaacgcg gcggcgtcgc cgaaggcgag ctcgagcctc gggtcgtcga acgccccgtc
    32761 gctgagggtc ggcaggaact tccggcagaa ctcgatgacc ccgggatcga gctcggcctg
    32821 gagcacccgc tcgacggtgg cgtgcttcat gacctcgcgc gccgtgccgc cgtcgccgcc
    32881 gccgacgatg cagacgctgc gcacccggcc atgcgccagg atcgccgggt gggccagcat
    32941 ctcgtgatag gcgaactcgt cgccggtcgt ggtctggacg acgccgtcga gggcgaggac
    33001 cgtcccccat cggccggtct cgaacacgat caggtcctgg tgctcggtct ggtcgtgata
    33061 gaggatcttc tcgaccttca tccgttgctg ccagtcgctg tgcagcgtct cggcgaacca
    33121 ctccatcacc tgcatcccgc ttccgttgtc ggagacccgt gccccacctc aaagatcggc
    33181 gtagcagtgc cgctcggcgg cgccgccggg gtgggtgacc gcccctttct cggccgagcc
    33241 cacggtctgc gcgtacttcc acaagctgcc ggcgccgtag atcgactgcc gcggctgcca
    33301 tttggcgcgg cgcgcgtcca gggtcgcctc gtcgagctcc aggtcgagcg tgccggcctc
    33361 ggcgtcgatg gcgatcgtgt cgccgtcctc gagcagcccg atcgggccgc cgaccgcggc
    33421 ctccgggccg acatggccga tgcagaagcc gcgcgtggcg cccgagaacc ggccatcggt
    33481 gatcagcgcc accttgtcgc ccatgccctg gccgtagatg gccgaggtga cgcccagcat
    33541 ctcgcgcatg ccggggccgc ccctgggccc ctcgtagcgg atgacgatga cgtcgccggc
    33601 cacgtacttg ccggcctgga cggcggccat ggcgtcctcc tcgcagtcga agacgcgggc
    33661 cgggccgcgg aacttcaggc tcggcaggcc ggccaccttg accatggccc cctcgctggc
    33721 gagcgagccc ctgaggccga ccacgccgcc ccaggtggtg atcgggtcgc tggtcgggcg
    33781 gaccacgacc tgcttcgtgt cgaaggccac gtcggcgagg ttctcggcga tggtcctgcc
    33841 ggtgacggtg atgcagtcgc cgtgcaggta gccgccgtcg agcagcgcct tcatcagcag
    33901 cggcacgccg ccggcctcgg ccaggtcctt catgacgtat ttgccggccg gcttgaggtc
    33961 ggcgatgtag ggcgtttcgc ggaagatggc ggcgacggcg tgcaggtcga agtcgatgcc
    34021 ggcctcgtgg gcgatcgccg gcagatgcag ggcaccattg gtcgacccgc cggaggcggc
    34081 gacgaccctg gccgcgttct ccaggctctt cagcgtgacg atgtcgcgcg gccggatatt
    34141 gtccttgagc aggcgcatga ccgcctcgcc cgaggcctcg gcccagcggt cgcgcgcctc
    34201 catcggcgcc ggggtgccgg ccgagccggg cagggcgagg ccgatggcct cggagacgca
    34261 ggccatggtg ttggcggtga actggccgcc gcaggagccg gccgaggggc aggcgacctg
    34321 ctccagttcc tcgaggtcgg catcgctcat cttgccgacg gcgtgctggc caaccgcctc
    34381 gaacacgtcc tggatggtga cgtcgcggcc gcggaaccgc ccggggagga tcgagccgcc
    34441 atacatgaag acggccggca cgttgagccg gaccatggcc atcatcagcc cgggcagcga
    34501 cttgtcgcag ccggcgaggc cgaccatggc gtcgtaggcg tggccgcgca tggtgagctc
    34561 ggtcgaatcg gcgatcacct cgcggctgac gagcgacgac ttcatgccct ggtggcccat
    34621 ggcgatgccg tcggtgacgg tgatcgtcgt gaactcgcgc ggcgtgccgc cggcccgggc
    34681 gacgccctgc ttgaccacct gcgcctggcg gccgagggcg atgttgcagg gggccgcctc
    34741 gttccaggtg gtcaccacgc cgacgaaggg cctggccatg tcggcggccg agaggcccat
    34801 ggcgtagtag taggagcggt gtggcgcccg gtcgggcccg acggtggcat ggcggctcgg
    34861 cagccggctc ttgtcgtaga tggtgacact ggtcatcggc gtcctctccg gcatcgtcgg
    34921 cctgcccggt ggcccgggcg gcgcaggcgt ttggccgcga accatagtca ggggccgtcg
    34981 acctgactag ataggagcca cgggccgcgc agcccgagcc acccgttgcc gttgcagcat
    35041 cgggcttgca ggaaacggga gacagtcagg cgcatggcgg gcggcagcga catggtgagg
    35101 cggcgggtgc gccgggggcg caaccatcgc gtgctgctca cgccgatcca gcctttcgcc
    35161 acgaccggcc tcgaggagcc gacctcgccg gtccgcaccc ggcggcgccg gccgcccgat
    35221 ctcggctggg tgcgccgcat cctccggccg gccttcgggc atttcgtcca cgaccagggc
    35281 ttcgtgctgg ccggctacat cgccttcacc gcgatcttcg ccttcttccc gttcctgatc
    35341 gtcatgctgt cgatcgccgg ctgggccggc cagggcgagg cggccggcca gttcatcacg
    35401 ctgaccctcg agctcctgcc gaacgaggtg gccagcgtgc tgcagccggc gatcgcggag
    35461 atccgctcgg cgccgcacgg gacgatcatc accctcgcca ttccggtgac gctctggttc
    35521 gcctcctccg gcctcgagag cctgcgccac gcgctgaaca tggcgcacga ggtggccgac
    35581 cccccgagct tctggtggaa ccggctcgcc agcatggcgc tgaccgtggc ctcggcgacc
    35641 gtgatcctcg cggcgatggt gctgatggtc ggcctgccca tcgccgagaa catcctggcc
    35701 tggctcgccg agcatcaggc cctcgaggag cgcatggggc tgatcatgcg ctacgggatc
    35761 ggcatggcgc tcctcctgct gctcaccttc agcctctacc gcctcctgcc gaaccgccgg
    35821 ctgacactgc tcgacgtgct gccgggcacg ctcgtctcgg tgaccttctg gctgggtgcg
    35881 accaagctct actcgaccta tctctcgagc atcggccgct acagcatcat ctatggcagc
    35941 ctcggcggcg tgatcctcac gctcttcttc ttctacgtca tggcctgcat cttcatcttc
    36001 ggcgcccagc tcaacgcggc gatcatgcgc gagcgggccc tgcagcgcga gcggcgggcg
    36061 gcttgaggag ccggcgacca tgacgctcgg cgcggctcgc gtcgaggcgg cggtgctccg
    36121 ccattccctc tggctcaccg tggcgatcgc ggcggtcggc gtcgtcttcg gcatcctcag
    36181 cggctcgctc gccatcatct tcgacggggt ggcctcctcg atcaacgcgg ccatgacctt
    36241 cctgtcgctg gcggtcgccc ggctcctggc gcgtgagggc agccgccgct tccagttcgg
    36301 ctactggcat ttcgagcctc tggccctggc gctcaacggc gccctcttgg tgatgttctg
    36361 cttctacggc ttcgtcaccg gcgtgagcga cctccttgcc ggtggccgcg atctcgattt
    36421 cggctgggcc atggcctatg ccgcgctctc cacggtggcg agcctcgtca cctggctcta
    36481 cgagcgctgg cgcaaccgca ccatccgctc ggagctgatc gggctcgaca tgcagagctg
    36541 gctgatggcc acggcggtct cggcggcgct cctcgtggcg ttcggctatg cactgatggt
    36601 ggaggggacc gcgctcgcgt ggaccgcggc gctcgccgac cctgccgtcc tggcgctggt
    36661 cagcctcggc ctcgccttca tgcccatcgc ggtcctgcac caggcctggc aggacatctt
    36721 ccagatggcg cccggctcgc tcgatgcggc ggtcgaggcg gccgcccggc gggcggtgga
    36781 acggcatggc ctcgcgacgt tcacgagcta cgtggcccgg gccgggcggg cgaccttcat
    36841 cgagatccat ctggtcctgc cggccgacta tccgatcgac agcgtcgcca cgctcgaccg
    36901 gatccgccgg gagatcgcgg aagagctcgg gcccgcgcga cccgagcgct ggctgacggt
    36961 cgccttcacc ggcgatgcga cgctgatttg aagagcgctt catcactgcc ggctcctcgg
    37021 gcatgcgcac cggtcccgct agagtcgggc cgtcggctct agcgtgctgc ggtccggcaa
    37081 gggttcgggc acctgcccca gcacgctgat cgtcccgtcc gtctcgagca cgacggcgcc
    37141 ggcctcggcc atgtcggcga agccccgcga gcgcacggcc gccgtcacct cctccagcgt
    37201 gatccgctgc tcgcgcatcg cccggtcgag cggcgatccc cgcagcatca ggagggtggg
    37261 ctcgctcttg accagccgcc ggaacgacgg ccagcgcacc gagagccagg tgatgaggaa
    37321 ctgcaggccg acgagcagcg tcagcgctgc gatgccttcg gccagcggca cggtcttcga
    37381 gagcaggacc gtcgccaggg tggagccgag ggccacggtg acgacgagat cgaaggcgtt
    37441 gagcttggtg agcgtccgct tgccggaaat ccgcagcacg acgacgagcg ccacgtaggc
    37501 gagggttccg accagcagca cgcggccgat gccggcccag gaatcgaaga tcatccatgc
    37561 tgcctttcgt tgtccgtcgg gtgtcttccc gaccacctac aacgcccggg cgacatggtg
    37621 gttcgctctt ccccttccgt tggcgtccgc aaggcactaa gtttcgcgcc attccatcaa
    37681 ccgccgtatc gaggagcgct cggccatgac caccggtgca ccgctcatgg ccggcaagcg
    37741 cggcctgatc atggggatcg ccaacgagcg ctcgctcgcc tggggcattg cccgggcggt
    37801 ggcgggtcag ggtgccgagc tcgccgtcac ctaccagggc gatgccctga agaagcgggt
    37861 gacgcccctc gccgccgagc tgggctgcca cttcgtcgag ccggccgacg tcaccgagcc
    37921 ggcgagcatg gaagccctct tcaccaccat cgagcagcgc tggggcgggc tcgatttcgc
    37981 cgtccatgcc atcgccttct ccgacaagga ggagctgaag ggccgctacg tcgacacgag
    38041 cgccgagaac ttccagcgca ccatgctgat ctcctgctac tcgttcaccg acctagcacg
    38101 ccgtgcggcg gcgctgatgc agccgggcgg cagccttttg acgctcacct attacggggc
    38161 cgagaaggtg gtgccgcact acaacgtgat gggtgtcgcc aaggcggcgc tcgaggcgag
    38221 cgtgcgctat ctggcggccg acctcggcgg ccaggggatc agggtcaacg ccgtctcggc
    38281 ggggccggtg aagacgctgg ccgcctcggg catcggcgat ttccgctaca tcctgaagtg
    38341 gaacgagctc aacgcgccct tgaagcgcaa cacctcgctc ggcgatgtcg gcggggccgg
    38401 ggtctatctc ctctcggacc tcgccagcgg gacgacgggc gaggtcctgc atgtcgacag
    38461 cggctaccac gtggtcggca tgaaggcggt ggacgccccc gacatcgcga ccgtctgaga
    38521 gcctgatggc gggcagcagc ttcggcacgc tcttccggtt cacgacctgg ggcgagagcc
    38581 acggggcggc gatcggctgc gtggtcgacg gcgtgccgtc ccggctcgag ctggccgagg
    38641 cggacatcca gcactggcta gaccggcgcc gacccggcca gtcgaggtgg acgacgcagc
    38701 gccaggagcc ggatcgggtc gagatcctgt cgggcgtgtt cgagggcctg accaccggca
    38761 cgccgatcag cctccttatc aggaacgagg accagcgctc gaaggattac ggcgccatcg
    38821 cccggagcta ccggcccggc cacgccgatt tcacctatga cgccaagtac ggcattcgcg
    38881 actatcgggg cggcgggcgg agctcggcgc gcgagacggc gatgcgggtc gcggccgggg
    38941 cgatcgcccg caagatcctg gggccgcaga ttgccatccg tggctgcatg gtgcagatgg
    39001 gcgccgatcg gatcgaccgg cagcgcttcg acccggcggc gatcgaggcc aatcccttct
    39061 ggtgcccgga caaggaagcc gtggcgcgct gggaaaagct gctcggccgg gtccgcaagg
    39121 cgggctcgtc gatcggcgcc gtggtcgagg tcgtcgcctc gggcgtgccg gccggcctcg
    39181 gcgagccgat ctacgacaag ctggacgccg atctcgccag ggcgatgatg agcatcaacg
    39241 cggtcaaggg ggtcgagatc ggggccggca tggcggcggc ggcgctttcg ggcgaggaga
    39301 atgccgacga gatgcgcaag gggccggacg gtccgatctt cctctcgaac caggccggcg
    39361 gcattctcgg cgggatctcc accgggcagg acatcgtcgt ccgcttcgcg gtcaagccga
    39421 cctcgtcgat cctcacaccg cgccgctcgg tgacggcgga cggcgacgag accgagatcg
    39481 tcaccaaggg ccggcacgac ccttgcgtcg gcatccgcgc cgtgccggtc ggcgaggcga
    39541 tgctcgccat cgtgctcgcc gatcacctgt tgcggcaccg gggtatattt ggcggttgag
    39601 atcgccgtcc ccgctttacc gcggcttcat cctgccatgc gataacgagg gtcaccggcc
    39661 cgatgcgtcc gtcgggggac acgaagtccc ctgcagcgtg cccgcaaccg cctcaagtag
    39721 agggaaacgc caatgcagtg ggtgcacaag cgctccttgc cgattccggc attgaccatc
    39781 accgaccccg ccctgatcgt cgcctatcgc tattgggccg agcgcaagga cggcgcccac
    39841 ctgcccccgc gctgctcggt cgacacgccg atcttccgcc tgctggtgcc cgacgcgctc
    39901 tggctcggca ccggcgctta cgcgggccgg ctcgcaccct ttctcggcgc cacgcaggtg
    39961 catgtcccgt cgatgaccct cgaggagagc ctgcgtctcg atatggacgc ggccgcggtc
    40021 accggggtgc ccctcctgca actggtcgag ctcaagatca ggggcaggcc cctcatctac
    40081 caacagttgc ttttgcccac gtccgacgac ggatgcaagg tgaacgaggt actcgaggtc
    40141 agcaagttgg gcgccttgca gttgatgggc agccgcccca ctagcgcttg aagcgtggga
    40201 atcggagtca cgcagcaaaa tgtgcgaaat gccacagatc gcacccggcg cgccgtgctt
    40261 ttagttttcc tgctgagggc tgagacttgc cgcctgtgag gacatgctcc tcacaggcat
    40321 ttttttgccg accccgctca gggctccagc gcggcatgac cgggtgaggg ttcgagccgg
    40381 ggattgccgg ccgtgcccgc ccctgccaca ttccgccggc gcatggcggc cgggtccgcc
    40441 ggcgggtttc ggaccattcg gggagggacg gcaatgcggc tcgcggcggc catggggcga
    40501 ctgggcacgg aaagcgcgtt cgaggtcctg gcgcgggcga cagcgctgaa ggcgcagggg
    40561 cgcgacatca tcaatctcgg catcggccag ccggacttcc cgacgccgcc gcatatcgtc
    40621 gaggcggggg tcaaggcgct tcgggacggc caccacggct acacgccggc caacggcatc
    40681 ctgccgctgc gcgaggccgt cgtccgcgac atggccaagc ggcgcggggt cgaggtcgat
    40741 cccgcctccg tcgtgatcgt gccgggcggc aaggtcacca tggccttcgc catgctgatg
    40801 ttcggcgagc ccggggccga gatcatgtac ccggaccccg gctttcccat ctaccgctcg
    40861 atgatcgagt tcaccggggc cgaggcggtg ccgatggcgc tgcacgaaag caagggcttc
    40921 gccttctcgg ccgaggaggt gctgggccag atcacggacc gcacgcggct gatcatcgtc
    40981 aacagcccgg ccaacccgac cggcggtgtc gtgccgaagg ccgagctcga caagttcgtc
    41041 cacggcctcg ccgacttccc cgaggtggcg gtgatgtcgg acgagatcta ttcgcgcatg
    41101 tgctacgacg gcgtgccgca cgcctcgctc acgacgtatc cggagatcgc cgaccggttg
    41161 atccttctcg atggctggtc gaagacctat gcgatgaccg gctggcggct gggctggggg
    41221 gtgtggccgg gcaagctggc cgagcacgcg acgaggctcg ccatcaacgt caattcctgc
    41281 gtcaacgccc ccacccaata tgccggcatc gccgccctcg acgggccgca ggaagccgtt
    41341 ggcagcatgg tcgaggcctt cgaccggcgg cgggcggtga tcgtcgagcg gctcaacgcg
    41401 ctccccggct ttcgctgcgc cgtgcccggc ggcgccttct acgccttccc caatatcgag
    41461 ggcacgggcc tcggctcgcg ggcgatgcag gacgggctgc tcaacgatgc cggggtcgcg
    41521 atcatcagcg gcaccagctt cggcgtgtat ggcgagggct acgtgcgctt ctcctatgcc
    41581 gcgtcgatcg aggagatcga gaaggccatg gaccggatcg cgggattcct cgccaaacgc
    41641 gccgcctgag gcgcctgccc tggccatgct ctgaattcct gtcgcggccg ccgttcggcg
    41701 cgtgtaaatt ggcgatcgaa ggtgcccctt cgaggcaccg ggcgggggaa cgggcgatgc
    41761 cgatcgagca gtctgccctg gccgcgattc gcagcgagct cggccaggtc ttgtgggatg
    41821 cgccgctcta ccagggcatt ttcaacgtcc agttggctcg cgccccgacc agggtgcgct
    41881 ttggcttcca gaccttctgg ccttgctacg ctcgggtcga ggtctaccgc atggtgacgg
    41941 gcgagctcga cctcgatatg gagaaggaga acctgctccg ggtcggctac gagctcttcg
    42001 gcagccccaa ggtgagccac aacgtgctga tcaccgagct cggccaggag cagcgcttct
    42061 ggtaccgcat ctcggtgccg cgaaagaccc ccgattttcc ggatgccaag ggcttcgtcc
    42121 gctcgcgggg cgagttcttc acgccacgcc gcaacaccat cgtcaccgtc gaccgcattc
    42181 acgtcatgga cgacagcgac acgtcgggcg agggcgagct gacgttcagc ctcggcctct
    42241 acaacgcctc cgacccgcgc gagcgtctgg tcgagcctcg catctcgccg aagcaggggg
    42301 tgagctcggg cgacgactac aatcggccgt tcggcgccgg cggccagagc atcggccgcg
    42361 cacccgattt catcggcgtc tacgtcttcg gcgtcgacga cgatgcgacc ttctggggtg
    42421 ctccctggta tgggctcggc atcgtcggca tgcgtccgcc ggccaccatg cccgatagga
    42481 ccttcaccga atcccgcgac tatgccgaca tgaccgacgc actgcacgtc ttcgccaccg
    42541 gggccaagcc ggggcgtcag gagggccgct tcgatttcag cagcgtcgaa ggccggctgc
    42601 atttctcggt gcacggtcgg ttcgtcaccg aggtggtcga cacgctgggc gagcgcatca
    42661 ccccgccctg gaaggcgttc ttcaagacca gcatggcaat cggcacgggc ggcaagcctg
    42721 cgggcctggt cggcctgccg gcgggcgcga tcggcgtcgc cctcgatccg gagggccggc
    42781 tccagctcgg cgaaggcggc agcctcgatc gctggcgcgc ggccgaggga ccggccttcc
    42841 aggcgctcgt cgccaaggcg acgcgggccg gcgcgctggt gctggtcggc cgcgatggcg
    42901 atggtgtggc gcatgtcgcc cgtctcctcg agccgcaccg cgacatgcct tgctggacag
    42961 caatcggtcg caccaccgac ggcccgccgg cgctgctcga gga
//
LOCUS       DAOTFI010000004        42388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_950_length_42388_cov_6.655682, whole genome shotgun sequence.
ACCESSION   DAOTFI010000004 DAOTFI010000000
VERSION     DAOTFI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 42388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42388
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_950_length_42388_cov_6.655682"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="P5558_00655"
     CDS             <1..258
                     /locus_tag="P5558_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22848.1"
                     /translation="WAVLASALLAATRHRLRLRPTTWRLCHTALAAVIVPASVVHALL
                     IEGTMGKLSKAAICALVVAAAAKVIVDRRAWVVLLRHNVQG"
     gene            270..800
                     /locus_tag="P5558_00660"
     CDS             270..800
                     /locus_tag="P5558_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRY22849.1"
                     /translation="MAKLVFGMNVSLDGYVDHMAFGPGPTLFRHFIEEAKAQSGSVYG
                     RRMYEIMRYWDDDHPEWDTERQAFAAAWRKQPKWVASHSLKSVGPNATLVRDDFEGAI
                     RRLKTERDGEIEVAGPNLAQSLTELGLIDEYRIYLHPVVLGKGKPYFAGPRPPLRLMS
                     CDRIDKDVVRLSYVPA"
     gene            complement(819..1610)
                     /gene="xth"
                     /locus_tag="P5558_00665"
     CDS             complement(819..1610)
                     /gene="xth"
                     /locus_tag="P5558_00665"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRY22850.1"
                     /translation="MRITTWNANSVRLREAALRRIVAEIDPDVLCLQETKVEDGKFPL
                     DLVHDLGFPHCHIHGQKGYHGVAVLAKLPLDACHSHKWCGIDDSRHAVCTLAGGIELH
                     NLYIPAGGDIPDPELNPKFRHKLAMLDELSDWFATNHYESRRAILLGDLNIAPLETDV
                     WSHKQLLSVVSHTPVEVEKLGRLQASAGWVDAVRAVIPPEEKLFSWWSYRNRDWNASD
                     RGRRLDHIWLTPALAPKLRHADVRREARGWEMPSDHVPVTVELAV"
     gene            complement(1634..2725)
                     /locus_tag="P5558_00670"
     CDS             complement(1634..2725)
                     /locus_tag="P5558_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY22851.1"
                     /translation="MTDTPIVTFENVTKSFGRTKAVDGVSLAIPRNAFFALLGPSGCG
                     KTTLLRMLGGFAHPTSGRVLIDGEDMADVPPNRRPVNMVFQSYAVFPHMTVAENVAYG
                     LKVTGVPKAEIGPRVEEAIAMVRLDGMAGRMPDQLSGGQRQRVALARALVKRPKLLLL
                     DEPLSALDKKLREEMQLELVRLQTEVGITFIIVTHDQEEALGMASDIAVMKEGRILQI
                     APPRQLYEHPTHRFVAEFIGASNSFECEVLGIEGGMARVRAPLLGELSVERASADVRA
                     GTLIVRPEKMRMDFSSPDPDPGEVLVEARIDQISYFGDLSVVYLTAADGRRLQCSRYN
                     TNRIDKGDPPPGTPCTIGIHPEDLLLVGD"
     gene            complement(2722..3561)
                     /locus_tag="P5558_00675"
     CDS             complement(2722..3561)
                     /locus_tag="P5558_00675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY22852.1"
                     /translation="MARREPIGPLDYTRRLWLRLWLLAVAVFLYAPIVILVVFSFNDS
                     KRNIVWQGFTTDYYVKAAANASLIEAFTNSLVIALVSTVISTIVGALMALALWRFRFP
                     GKPGLDGAVALPIVIPEICMGVALLVFFNRVGVSAAFGWPFSLLPIIIGHVAFSFPFV
                     CVVVRARMAGFDRSLEEASKDLGASEWQTFWNVTVPFMKPGLIAGGLLAMTLSLDDFV
                     ITFFTAGPGTLTFPVQVYSMVRFGATPEVNAASTILIVVTVILTVIALRLQRPDKEAA
                     KLA"
     gene            complement(3548..4465)
                     /locus_tag="P5558_00680"
     CDS             complement(3548..4465)
                     /locus_tag="P5558_00680"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY22853.1"
                     /translation="MESWRAQPAVFWAFVLPGALWLLCFFIAPLALIWLLAFGERAGP
                     VEIDITWTLANYAGVFDPVYIGLFGRSLWVATVATVLALVIGLPVALAISFAPARLKP
                     LILLLIILPFWTNILIRTYAMIAVLRTKGFVNFGLEYIWQLGTVVLAPFGLDQAVMGE
                     RFVPLELLYNNTAVIFGIVYVYLPFMVLPLYATLDRLDRSLLEASLDLGAGQIRTFFS
                     IIVPLARPGIVSGIILVFIPALGTFLISDLLGGTDSLLIGNVIERQFKAANNWPLGAA
                     MSFVLLYVTFAVLALRAWVAGREGGLGKA"
     gene            4584..5792
                     /locus_tag="P5558_00685"
     CDS             4584..5792
                     /locus_tag="P5558_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY22854.1"
                     /translation="MPFEQDVEIAVVGAGITGLAMALALARANAGTGAEVALLAAAPL
                     PTSRAEAADGRTAALLMPAIGLLTRLGVFDPTGLGAEPLYRLRIVGGSGKGAAEGTAL
                     TFDAAELGMEAFGWNIPNATLAARLAAAVGRTAGIAVHAGAPLQNVTEAGDRLLLEAG
                     DTRLAARLVIGADGRDSMVRETAGITTTQRDHGQTAIVCRFAHTKPHRNISTELHRQG
                     GPFTMVPLPGRNSSLVWVDRTAESEARLDLDDDAFREAMAPLAEPWLGPIGALSRRHA
                     YPIRARLAKALTAPRRALVGEAAHVMSPLGAQGLNLSLRDVATLAGLLAAHPGDPGDP
                     ALLAAYAEARAVDIKARFWGIDTLNHMVMQSLPPFRLASSLALGLLDRLPPARRALMQ
                     GMMEGVPDGL"
     gene            5869..6303
                     /locus_tag="P5558_00690"
     CDS             5869..6303
                     /locus_tag="P5558_00690"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HRY22855.1"
                     /translation="MEEITMALATPRAAAPAGLDALGQDPKLQADLMRGFFGMMRLWR
                     VENADARRILGDPAERTFYGWKGGKVGRLPADTVRRIGYVAGIFKALQILYPDSRQAD
                     QWIRQPNRHFGGQTPLARMAAGDVTDLAAVRAYLDAARAPWS"
     gene            6278..7024
                     /locus_tag="P5558_00695"
     CDS             6278..7024
                     /locus_tag="P5558_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRY22856.1"
                     /translation="MPPAPPGPEAPDPDPEAEPALVRVRWDHATRIIASRYPPIDLFE
                     RVDGDPAVWEALIAAEMLTNPRIRDGLGEIALVAPEERVAGPGATYVMASFTHLNPKG
                     SRFSDGSYGVYYAAIDFPTARAETIYHFERFARDSGDAVRDTDMRVVVGKVDERFHDI
                     ASLPGPRRTAVLAPDDYTSSQALGRRLREAGSNGVVWPSVRHAAGTCLGAFRPKAVQP
                     PVQERHIRYHYDGRRVSAWFDYATSAWHAL"
     gene            complement(7040..8005)
                     /locus_tag="P5558_00700"
     CDS             complement(7040..8005)
                     /locus_tag="P5558_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY22857.1"
                     /translation="MTSAGGPARSDIEIAIIGPQRPALVAALEARFKCHNVHAEPDPL
                     AYLDQHGARFRGAVGSGMAGLQRQHMDRMPNVEICAINGVGLEMTDLALAREKGIAIT
                     ITPVLYDDVADLALALALAACRRIAHGDRFVRNGDWLKGRLVLGRKLTGMKAGLIGLG
                     RIGSEVASRLEGFKCEIAYVDPVARDVPYRRVADAVTLASESDILFLCAAGAARGTAA
                     PIISRQILEALGPRGIFVNIARGWLVDEPAMVELLASGGLGAAGLDVFDDEPRVPEGL
                     LGLDNVVLTPHIASSTEETMAAMGDTVVANLVSWFEGKGALTAVP"
     gene            complement(8130..9206)
                     /locus_tag="P5558_00705"
     CDS             complement(8130..9206)
                     /locus_tag="P5558_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY22858.1"
                     /translation="MPRRLSPFPAPMLNRRRAIQAGLAVAAVAGALPRLPLAQGGELN
                     VYNWDTYIGPDTLDDFTAATGIRVRYDLYGSSDEMFGKLRDGNPGYDVVYPSNDFVER
                     MIVADMLLPLDHARLTNFGNLAPRFQNPSFDPGRRFSIPYFWGTMGIGYRQSVASPDS
                     WAALFESDEFAGRISVLNEKNLIQMALLYLGYSLNTTDPAEIDAAADLLIRAKPNIKA
                     FTPDTGQDLLIAGEVDLCMEWNGDILQVMEEDDDLAYVVPKEGGLLWEDDMAIPRGGP
                     NPDAALEWINFNLDAEVFGAIATYVRFPSPNEAAKAYIPAEDLENPSMYPPDAVLDKC
                     EVSIYKGEAVESLYENALTRVLAA"
     gene            9319..>10135
                     /gene="rmuC"
                     /locus_tag="P5558_00710"
     CDS             9319..>10135
                     /gene="rmuC"
                     /locus_tag="P5558_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRY22859.1"
                     /translation="MTDPAFWPWLLAAFLLGLVLGIGVAALLGRGQARRLMGVMGEAQ
                     RRHEAETDALLDGVKLAFHDIGRELAGASRDDMLRLAQTQLAGERRALGHTLEAERGE
                     LAARIATMLTQLERTQGLIRELERDRTGKFQALATQLRVAGDSAAELGRTTRHLARSL
                     SGAKLRGQLGERLAEDVLKAMGLTPGIHYTRQAPLDAERRPDFLLALGDGRRLAMDVK
                     FPLDNWRRAVEAADEETQARLEAQFMRDVRAHVAAVASRGYAAPELGTHDFALL"
     assembly_gap    10136..10235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10236..10590
                     /locus_tag="P5558_00715"
     CDS             <10236..10590
                     /locus_tag="P5558_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22860.1"
                     /translation="GPSTLYALLAALRMAEGEARLARAGQDLARAIGGFRLAWTRYAE
                     RAQRVERRLEDTLDELRKLYGLRRATVDRAMATVEAVLDGAPPEPDTADEPQDEAQGA
                     AKSASFSGGRALTSE"
     gene            complement(10533..11378)
                     /locus_tag="P5558_00720"
     CDS             complement(10533..11378)
                     /locus_tag="P5558_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRY22861.1"
                     /translation="MHCSDLESYLEACLDGQLGEARRDALRRHLALCRPCSERVDELR
                     LFEADLQRRLRAMQHEVSLWAPLGLEMTTEPVRESQPILFHPPVAQAGPVVPRPARAG
                     QHRLRLGLARQRPAAVRRPVRRRLQTLAGAALMVAAVGAIVDLATGWLGGSRNIQLYR
                     AYVAGDLVLDLRTGEPKELAAWLDRELGAPIELPAVAGFGLVGGSAELPGGPDSAAVV
                     FAAEGVPVLLVIEPGANPADAAVAPPALAVEDGLTRLDWHEGAHAYSLVSALPPEKLA
                     LFAAP"
     gene            11635..13668
                     /locus_tag="P5558_00725"
     CDS             11635..13668
                     /locus_tag="P5558_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY22862.1"
                     /translation="MGAAPTIRNVALVGPAGSGKTTLLESLLFVSGAVPRKGRVADGS
                     TIGDPSPEARARQMSTEVTAAAFGHGDHRFTILDCPGSVEFAAEARAALLGIDLAVVV
                     VEPVAERMSAVAPLLHHLDSAGLPHVVFINKMDRSEVRYRDLLDALRQVSTRPVVPHQ
                     YAIGRGEELVGYIDLVTEQAYAYKDGAPSDRIDLPDDYREREQEARAEMLETLADFDD
                     DLMEKLLEDQEPSTEEILKDLQKTLKADQVVPVFMGVAEKDMGVRRFLEALEAEAPEP
                     AERATRLGLDPDGPTIVQILKNYHQPHAGKLSLARVWSGTVKDGMQLGGMRVGGVYRL
                     FGAQQEAAGEAAAGEIVALARMDEAKPGAVLGNGGADEAAATLPALDTGQPMFAFAVT
                     AANRNDEVKLSAAFHKLCDEDPSLALEHDAENHQTLLWGHGEIHLKVALDRLKGKYHV
                     EVEPHTPRTGYKETIKKGTSSHGRHKKQSGGHGQFGDVKIDIRPLPRGEGFAFTNKVV
                     GGSIPKQYIPAVEAGAKEFMAKGPLGFPVVDVGVTLNDGQFHTVDSNELSFKMATALA
                     LKEGLPNCSPVLLEPVLAVTISIPTDATSKALQLISQKRGQILGYEAKAGWSGWDEIK
                     AHLPQNETHDLIVSLRSMSQGTGFFTWSYDHLQEVPQKLAETLAAQAKEDAAA"
     gene            13808..14008
                     /locus_tag="P5558_00730"
     CDS             13808..14008
                     /locus_tag="P5558_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22863.1"
                     /translation="MPAEIDFSKGTRGKFAKPGTTLKLPVYLDADVEAYLTARASARG
                     VDVDQLVNELLKKDIELIEAAG"
     gene            complement(14382..14693)
                     /locus_tag="P5558_00735"
     CDS             complement(14382..14693)
                     /locus_tag="P5558_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22864.1"
                     /translation="MKLIATVLAVFVVVFAISFNVVGGIVGVVIGAAIAKYFLGFKIM
                     LNEQKIGLITLWIVLLSILGSMFVSEIDIAIISFFMAFSISCPSAALAIWLNRESSNS
                     A"
     gene            15094..15576
                     /locus_tag="P5558_00740"
     CDS             15094..15576
                     /locus_tag="P5558_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY22865.1"
                     /translation="MDTGTFHGRKRRKHGMPLGASWSHSDHSANVGRAMSPPSIHDRK
                     TFGPGKLLIRPGDPPDQAYLIQSGRARVFDTIDGKRRELATMGPGEIVGDMALIRKSD
                     HLHGVEAIDSVVAVVISIQRLHKALDGADPLLKTILEGMLARIDRLNEQKQPGDIWLV
                     "
     gene            15815..16132
                     /locus_tag="P5558_00745"
     CDS             15815..16132
                     /locus_tag="P5558_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRY22866.1"
                     /translation="MEPVHVMAVIEAKPGKRAELLAAFKANVPAVHAEDGCIVYEATV
                     DTEAAGPIQNATFGADTFVVVEKWASLEALEAHAAAPHMKSYGERVKDLIAERTIYVL
                     SPA"
     gene            16198..17418
                     /locus_tag="P5558_00750"
     CDS             16198..17418
                     /locus_tag="P5558_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY22867.1"
                     /translation="MYQNAALLALLSFLYVLISGRVERSWLSGPILFVAGGFLLGPGG
                     LAVLSLEMTAEGLRLLAELTLAMVLFSDAATADLARIRRSRGLPERLLLIGLPLAILA
                     GMGVAWLIFPGIGLVEMALLAAILAPTDAALGEPVVANREVPPDLRATLSFESGLNDG
                     ICVPVVVLLAGFAIGTEIEGQPAAHVLRVIVEELGIGLATGAVLAGAAAALLRLAHAR
                     NWIADGWRDLALVAVAIACFTTAQALGGSGFIACFVGGLVVRLPRPRRHELLRGTHIV
                     GDGLALLTWVAFGAAIVWQVADRVTLPVVLYALLSLTVVRMLPVYLCLMGSKLAPRDR
                     LFVGWFGPRGLASIVFALLILDTGLPHLEMLEAVIACTVLLSVLAHGMTANPLVRLCA
                     RRWRRNPGTATSQP"
     gene            complement(17918..18976)
                     /locus_tag="P5558_00755"
     CDS             complement(17918..18976)
                     /locus_tag="P5558_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRY22868.1"
                     /translation="MPAERWTADALTGFAEALCRKAGFDDEKAAVTARLLVEADMLGH
                     TTHGLQLLAPYLAAAEAGAMRTTGEPEVLADRPAALTWDGDRLPGVWLTDKALGVAID
                     RARELGTAMVAIRRSHHIACLAAFLPRATDAGMMAIISSSDPSVASVAPYGGRQPLYT
                     PDPIAIGIPTDGDPILVDISASITTNGLSGRYAAEGRRMPHPWLLDGEGNPTDDPNVL
                     TASPPGSILPVGGLDHGHKGYGLALMIEALTQGLPGFGRADPGEGWGASVTVQVIDPA
                     CFAGSDAFNRQTGWLAGACRANPPRPGVERVRLPGENGLARKRKALADGVELYPGIMT
                     KLTEVAGTYGVEAPKPLA"
     gene            19100..20278
                     /locus_tag="P5558_00760"
     CDS             19100..20278
                     /locus_tag="P5558_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY22869.1"
                     /translation="MQISDLIQQARRLTRQKQPAGVSLVHLAASPYIMANAARYHFFR
                     NSIAELATAHAADLHDLGRSRLCLVAADAAGIAHLALRRLLELETEAWNDGRSVDDIL
                     EGFRLPDQYMELRERLDVLERAGRHGADDGDAAARPVLEDRLAGPLTPALLARIEDRL
                     DGIDLAPFVRRQAVYRRGEEWEVAYTEHFTSLDGLKVALFPEVELRPDEPLFFELCRH
                     LDRLMLLALLLNRPWRQQRIGLNLGHAAWATEEFRRLLDRLDAAERGRLTIELHWMDA
                     LRDQAAGGAAMAEMRAAGIRIAIDRIGIESLPLLNLDRLEADHLKLVFDKGRLPLLLE
                     PAHLGALRRCRTDRVVLTQCDDPLALSLGQQLGITHYQGWLIDRLAREAVPAEERRVA
                     "
     gene            complement(20456..21277)
                     /locus_tag="P5558_00765"
     CDS             complement(20456..21277)
                     /locus_tag="P5558_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY22870.1"
                     /translation="MNRRSLAFVAFAATCAMALPALAQERFITVASTTSTEQSGLFGH
                     ILPQFEAATGIEVRVVAQGTGQALETGRRGDADVVFVHARSAEEAFVAEGAGVERHPV
                     MYNDFVIVGPADDPAGIEGKDDVVEALAAIAAAGATFASRGDDSGTHKAELRFWQEAG
                     IEPAGTWYRELGSGMGPTLNTSAELPAYTLTDRATWLNFSNRGPLEILVEGDPELFNQ
                     YGVILVDPARHPHVKAEDGQAFIDWLISDEGQAAIADYRIGGQQLFFPNANDAGV"
     gene            complement(21286..22065)
                     /locus_tag="P5558_00770"
     CDS             complement(21286..22065)
                     /locus_tag="P5558_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY22871.1"
                     /translation="MSAVLTGPVQLRRAGEASRPAPASILPLRLQGLGFAIAGKQLLD
                     DVDLVLEAGRRTVVLGPNGAGKSLLLRLCHGLLRPTQGRVLWSDNDADGPARRHSLLF
                     QRPVMLRRSARANVLHGLALAGLGWHDRRERALAVLARFGLADLAERPARNLSGGEQQ
                     VLALARAAALEPEVLFLDEPTAALDPAATARIELLLQLMARDGVKLVMVTHDLGQARR
                     FADEVVFLHRGRLIEHRPAGAFFSAPRSPEARAFVAGELIW"
     gene            complement(22062..22769)
                     /locus_tag="P5558_00775"
     CDS             complement(22062..22769)
                     /locus_tag="P5558_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY22872.1"
                     /translation="MAETSAFTLALQLIGAADPVLVEIVLLSLRVTLTALALAALIGL
                     PLGAALALARFPGRGVAIVLANALMGLPPVVAGLVIYLVLSRSGPLGSLGLLFTPTAM
                     VVAQSVLILPIVVSLTRQVIQDLWGEYAEQLRSLGSSPLRAVPTLLWDGRFSLITALL
                     AGFGRASAEVGAVLIVGGNIAGHTRTMTTAIALETSRGALELALALGLILLGLTVTLN
                     ALAYLLARLDRWGASVP"
     gene            22857..23780
                     /locus_tag="P5558_00780"
     CDS             22857..23780
                     /locus_tag="P5558_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY22873.1"
                     /translation="MMKLLTTAECAAYLRLKERSLYELAARRQIPCTRATGKLLFPLH
                     LLDRWLEARTEGSQDPGVASPLIYAGSSDPLLDWALRESGAGLAVLASGSLAGLGQLA
                     AGGARLAGLHLPPIDAADALTPDDGAAAVDPASSGNVAALRAALPFADVVAIRWAVRR
                     QGLLVAPGNPRGLAEATDLLEPGLRCAIRPPDSGSGVLLSRLLEAAGAAAVPTDPTLL
                     ARTEADLAALVADGRADCGLGIEAVARRFGTGFVPLATEAFDLVMRRRDYFEAPLQRL
                     VAFARSTVFAAEAERLGGYDLESAGRVAWNG"
     gene            23876..26041
                     /locus_tag="P5558_00785"
     CDS             23876..26041
                     /locus_tag="P5558_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRY22874.1"
                     /translation="MRQVVLFWLVVAGVLFASPASTVHAQSDAPPPAGVIELQRLLAE
                     PDVQAWLRQAVPQAAPAPSEAEAIVDEEEVYLEEALQRWSEHRSTITEALPELPGAIA
                     DALGRLSAEAADRGTLLVLLLVVVFVGAGLLAEWVFSRLTRPLRRGVLTAGEETIKAR
                     LQKLALRLALVLGTVLVFAAASLGAFLLLELPPLLRQVAGRVLFAIILFRLVFYLCRL
                     MLAPTAPQLRVMPIDDAWASFWFRRLTVLAGVYVFGWAAATSLRTLGAGYPYSFIIAY
                     LFGLALFAVVVTTIWQRRAHLVRSGLGARTIAAIAWTVLALAVLLLWVGGAQRLMWLL
                     LVGSLLPLAIHVTNASVNNLLRPAAGQVVVEPVPGTEALATSPEDEPVPVAGPPSVTA
                     AAIERGARALLIVGAAWLLIWAWDLDLPELMRGEGNFAPLARALLNIALIVIIFDFVW
                     HLAKTAIDGYVRIAATTAPANADEARRRARLRTLLPIARTALLAVLAVTAVLMILSAV
                     GVQIAPLIAGAGVVGVAIGFGSQQLVKDIISGVFYLLDDAFRVGEYIVAGSYKGTVES
                     FSLRSIKLRHHRGPLYTVPFGTLGAIQNMSRDWVIDKLSFTVPFNADVAKIKKVVKEV
                     SKQLLADPELGPGFIEPMKSQGADLIGDSGIVVRIKYMAKPGTQFPIRRRANAMLMQA
                     FAENGIEMAFPTVRVAGEGDSAAAAKAAIDAKAAAEAAE"
     gene            26132..27253
                     /locus_tag="P5558_00790"
     CDS             26132..27253
                     /locus_tag="P5558_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY22875.1"
                     /translation="MVRLLGLALLLPGLAMAQQPPPAPAVTVVAVESSDITPSVSFTG
                     RIEAVDKVDLIARVDGFLEARPFTEGDMVQKGDLLFAIEKGPYEARLGEVDAAIASAQ
                     ASFKLAQIEVDRQTVLVQREAAAQAVLDRAIAEADQARAAVQSAEATRADAELDLGYT
                     DIRAPFDGRIGLASVSVGAYVGLDSGSLATIVSQDPIYVTFPVTQRQLLAFREGAAGG
                     DTSTIAVKVRLANGTLYDQVGQIDFVDVEVDTGTDSITVRAVLANPDSLLIDQQLVTA
                     VVETAAPQTALLVPQQAVLSDQGGRFVLLVDADNKVEQRPIEVGQTQGANYVVTKGLE
                     EGDQVITDGIQRVRPGIVVDPGPPPDAAPEPAASPDSGN"
     gene            27258..30425
                     /locus_tag="P5558_00795"
     CDS             27258..30425
                     /locus_tag="P5558_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRY22876.1"
                     /translation="MISAVFVDRPRLAMVISIVITLAGLIAMLAIPTAQFPDIVPPQV
                     SVTTTYPGAGSDVVEATVGQPIEAKVIGVENMLYMKSTSGSDGSYTLTVSFTVGSDAD
                     INTVNVQNRVAQALSQLPQEVQRQGVTTKKKSSAFLQMVSLYSPDESRDVLFLSNYAT
                     INIIDQIKRIPGVGDAYLFGGLDYAMRIWIDDQRLTALGLTPNDVTQAIQAQNVQAAV
                     GRIGAQPMADAELQINITTKGRLTDVEEFSDIVIRANPDGSYVRVRDVAEVGLGAKSR
                     DVAARFNGGDGAVIAIQQLPGSNAIEAAAGVRELLEELKTRFPAGVDYAIPYDTTLFV
                     QASIEEVIHTLVEAFVLVIIVVFLFLGNARATLIPLIAVPVSLIGTFAVMLALGFSAN
                     TVSLLALVLAIGIVVDDAIVVVEAVEAKLEAKPELSVADATKEAMSEITGPIIAITLV
                     LLSVFVPVAFIPGISGQLYQQFAVAVSVSMVISAINALSLSPALCSVLLSHHHGPKRG
                     PMRYVMAAIDKTRDGYANIVGRLVRVAVVSLVLLAGFMVGTGWLAGVVPTGFLPDEDQ
                     GALFIEMQLPEGASLNRTIAASRKVEDVVRDKPGVADVTVVNGYSFIDGLAKSNSAFL
                     VLTLADFAERTAPEMRVDHLLPDLRREFASFREALVIPFNLPPIIGLGTGAGFEFQLQ
                     DLQGGSSADLASVTRGLIVAANQDDRLTGVYTTFSAATPQIYLDIDRERVQTLGIDVS
                     DVFNALQSTLGGYYVNDFNLFGRTWQVLVQGQEQDRAVVDDIYRIHVRNDAGQMVPVR
                     AFAEPRLTLGPQFVTRYNNFRSVTVAGTPSAGHSSGEAITAMEEVAAETLPAGYGYEW
                     TGTALQEIEAAGQTSTILGLAVLFAYLFLVALYESWMIPVPVLLSVTVGLFAAMLGLM
                     LFGLDNNIYAQIGIVVLIALAAKNGILIIEFSKMRREEGHPIGEAAVMGARERFRAVM
                     MTSFAFIAGLYPLVTATGAGELSRRGVGTSVFAGMIGASLVGIFMIPTLYVCFEWVRE
                     NSTRLFPWRHKPAAESHKPAEKAAP"
     gene            complement(30422..32152)
                     /gene="sulP"
                     /locus_tag="P5558_00800"
     CDS             complement(30422..32152)
                     /gene="sulP"
                     /locus_tag="P5558_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HRY22877.1"
                     /translation="MNGGLVAAPATKGWQRWLPGIATARHYELAWLPHDLVAGLVLAA
                     MLVPVGIAYAVASGLPGICGLYATIVPLLVYALFGPSRILVLGPDSALATLILATILP
                     LSGGDPVRAVALGGAMAVVAGLVCILAGLLRLGFVTELLSKPIRYGYMNGIALTVIIS
                     QLPSLLQVSIESTGPLLDLVAIAGAVLDGAANGPAAAIGIGVLVLILCLKHGTRFSGI
                     LLGVMVATVLVAVLDLGETADVIVLGALPQGLPAFALPWPTPADIVPVVVGGCAVALV
                     SFADTSVLSRSYAARLKTSVDPNQEMIGLGAANLATGFFQGFPISSSSSRTPVAESAG
                     ARTQLTGVIGAVGVMLLLVLTPGLLSDLPKSALAAVVIAAAIGLFEFADLGRLYRIQR
                     WEFWLSMACFAAVALLGPIEGIGLAIVAAVIEFLWDGWRPHWAVLGRVDGLRGYHDLK
                     RYPKARLVPGLVIFRWDAPLFFANAEFFQENLTEAIASASGPVRQVIVTAEPITSVDV
                     TAADMLAELETRLREQGIELRFAELKDPVKDKLRRFELLDQFGDASFFPTLGAAVDAY
                     LATHRIDWTP"
     gene            complement(32149..32649)
                     /locus_tag="P5558_00805"
     CDS             complement(32149..32649)
                     /locus_tag="P5558_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22878.1"
                     /translation="MCRARAFIDGAASGSPVGPRHYRQSHRAIESSAHDLGLSNIFIG
                     VFVIAILGNAAEHATAITAALQNRMDLSLSIAIGSSVQIAFFVAPVLVLAIPGIVPAP
                     VDLVFGKGQVLVVLLAVLVIAQVAGDSRSDWRKGAQLLAVYVIIGLAFFLTPQSWWRR
                     SSGGPE"
     gene            32671..33636
                     /locus_tag="P5558_00810"
     CDS             32671..33636
                     /locus_tag="P5558_00810"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRY22879.1"
                     /translation="MLQATLSSYRIITLPDPRSLVPPDRLRSLARRRDMPGLVFLGLH
                     LVLLGLTGTLVILMLGSWWLWPAMVVHGIVIVHLFAPFHETAHSSAFRNRRLNTTVLW
                     ATGLVLGLPPTFFRLEHAAHHAHTSDPGKDPEMVAASGSVAGYLWYATALPYFWALFS
                     SLFRHPFGAFTAVEQAFIPASQRRRVQREAIWMWAVYAAIASASIATGSTLTLTCWLV
                     PRVLGEPFMRIIRMSEHGACPLVPDMLVNTRTVLTLRPIQWLNWNMAFHAEHHAIPAV
                     PFHALPALHRDLGPHLAALERGYSRTQAVILGAAWSTREPKRAAA"
     gene            33682..35109
                     /locus_tag="P5558_00815"
     CDS             33682..35109
                     /locus_tag="P5558_00815"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004939.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRY22880.1"
                     /translation="MQKVDVAVIGAGTAGLNARSEVARCGGSVLLIEHGPYGTTCARV
                     GCMPSKLLVAAAERAQAVRDAGVFGVHAAGGLEIDGRAVMARVQKERDRFANGMVRST
                     EALPEDQRLRGHARFVGPTSLEVEGERIEANAVVIAAGSSPWLPPPFDDHADRVLTSD
                     TIFELEDLPESLAVIGTGVIALELGQAMAQLGVEVAFFNPFDDLGPFSDPDLMEAARA
                     TLGAGLELHLGAEMQGVEATGPGLRLTWRTADGERHARRFAAVLVAAGRRPNLAGLGL
                     EATGLRLDDKGRPGWNRRTTQCGDLPIFMAGDIAGGTGLLHEAADEGRIAGANALLYP
                     AVEAHNRRAPLAIAFTDPQMAIVGQRFADLDQGEVAIGAVSFANQGRATVMAKNEGLV
                     RLYARRGCGTFLGAEMLGPQVEHMAHLLAWSCQQELSVPRMLEMPFYHPVLEEGLRTA
                     LRALAKELDMVSTRRCEDAAEQPCS"
     gene            complement(35117..36535)
                     /locus_tag="P5558_00820"
     CDS             complement(35117..36535)
                     /locus_tag="P5558_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY22881.1"
                     /translation="MIPFPPFFDLLAQTTAVLVIVVSMAQNLLYLVLLLTAVSVMLGR
                     PRVHQSRALWNGLVDGAPPVSVIVPAYNEAETIADSLRSLLALEYPDFRVVVVNDGST
                     DATLDVLMREFGLEPAPLEHVSTLPHAPARGLYRSTRHANLVVLDKVNSGKADALNAG
                     LGQVTTELFCAVDADSLIEADGLLRVVQPFVEDPERVVAVGGTVRVANGCGVRAGRVR
                     AVALPRRMLPLLQTVEYLRAFMMARLAWGRIGCLMIVSGAFGLFRRDVVIELGGYSTK
                     TVGEDLELVVRLHRRMCNAGRDYRIEFVPEPVCWTQVPERLGELARQRQRWQRGALET
                     FFRHKDMLVGRKYGRVGRLGLGHILLLDVVTPLVEVLGYLLLPFFWLAGTINTPFFLA
                     YLALTFAFGVFISVASLVLEEFERRRVPRARDLVVLTAAAIIENFGYRQLANFWRIQG
                     HWQYWRGAAHWGRQDRRGFAAA"
     gene            complement(36532..37524)
                     /locus_tag="P5558_00825"
     CDS             complement(36532..37524)
                     /locus_tag="P5558_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22882.1"
                     /translation="MLGLGSLDFLMRLIWQATLVLVAIGLILIASLVLRRLLEEWAAA
                     RHRPAREGLRRALLASLNRPSGDPVAAPAGLPVAEIARLVDELAQIVRGDAKARLAAF
                     AVGAGIERYWLRRLRSATTLRRLDAVRCLGLLATDPARGALTTMLAQGDARHRLAAAE
                     ALAQDTALAAWLVERLLVDPASRGRHAARFWHRLAANAPETVVTCLARADVDAALLIR
                     LLEALGDAGHTAAATEIEALLGRHGAAVDRTALVTLDRLNHPAVLRLARALGGAPEAE
                     SRRAALGVLERRGRARDAELVSSLALDPVVDIAANAEKLLARLRAATADGAVPA"
     gene            complement(37524..38891)
                     /locus_tag="P5558_00830"
     CDS             complement(37524..38891)
                     /locus_tag="P5558_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HRY22883.1"
                     /translation="MRIDLRLPSKSGWPALVLLGGLLLSPLTSAPAMGAVPSVSRETL
                     LQLRADGEFEQALEVVDALLAKTPDDADLLLIKGQILAFLGDDRSALETLAEAARLAP
                     DYLDIRLMQARVHGYAERHDAALAVLRSAVTPEVARFDAQLLLGRTALAAREPEVARD
                     AFARAAVLEPDSGDAWLGLGDVARSEGVINVAQLNYERALVVPETAPVARERLDTLAA
                     EQRRFELSTDLSFSRFNDDSDDWREGGISLAWRIDDSRQLTGGLAVANRYGSTDIQAA
                     VVWNGRLDARSGYFLGAAVTPAANFLPSWKLRGGLDRELFDLTGRVGRLGIGVGFVEG
                     SLADYEDGLVQGFDLGLVQYGWEGRVWLTAKAGGSFGTNGSFDPNYGLRLDWQLTPDS
                     RVFGGFGQSYDNSTQGMGTTDSYFVGLDHWLTDRLGVALSLALEDRDNGVRQTTVALG
                     FRVRF"
     gene            complement(38888..39256)
                     /locus_tag="P5558_00835"
     CDS             complement(38888..39256)
                     /locus_tag="P5558_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY22884.1"
                     /translation="MARVLVVDDDELLVELVTFTLEGHGHTVLTAEDGADAFEIAVRV
                     RPDVMVLDGMMPGADGLDVLRRLRREAGLDLPVIMLSARKQERDVVGGLAEGASDYLV
                     KPFMPEELAMRIQKALRVPA"
     gene            complement(39377..40339)
                     /locus_tag="P5558_00840"
     CDS             complement(39377..40339)
                     /locus_tag="P5558_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRY22885.1"
                     /translation="MTSATTTDEQPSVLVVWPIRPNAMAQLEQEFRLVHLSSSPDPEA
                     LIEAESGDIRGIVSTGERGVSAALIDRLPRLEIVACFGVGYDGIDVGACRQRDVRVTN
                     TPDVLSKEVADMGIGLILMTLRRLAEADRFVRRGAWLEGAMPMGTCVTGKKLGIVGMG
                     RIGQELAKRAQALEMIPAYHNRNRRPDVDLPYFATPVELARWADVLVLACPGGPATHH
                     LVNESVIEALGPQGWLVNISRGSVVDEPALVKALEEGRIRGAGLDVFADEPRVPEALL
                     KMENVVLTPHHASATDETRDAMAQLVVDNLRAHFAGRPLLTAVA"
     gene            complement(40421..41053)
                     /locus_tag="P5558_00845"
     CDS             complement(40421..41053)
                     /locus_tag="P5558_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HRY22886.1"
                     /translation="MGTAKWLRSLTLALVLLVVACTAVQPIRYYALTPVSQESPVGET
                     DKVIGVAPIALPDYLDRNGIVTRTSPNELTVAENSNWIEPLDLQMRRVVARNLALLLG
                     TDLVFILPQQRLLPLDYTVEIAVERFDIEMGEAVPDAEAVPELTVFEGRWTLFRRESL
                     DVLKTAPIRITVPVEGPATFEKRTAAMSEAVARLSRTIATALAQAPKPAA"
     gene            complement(41059..>42388)
                     /locus_tag="P5558_00850"
     CDS             complement(41059..>42388)
                     /locus_tag="P5558_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRY22887.1"
                     /translation="RFWVVRPRVGFSGVTGLGTLLSGAYIEVEPAEGASATSFVGLEE
                     PPLIRSDVEGTQFKLVAENLGSVDRGAPVYYRGFEVGQILGHKLSDDGKRFEIPIFVR
                     SPHDKLVVPDSHFWDVSGISFRAGADGIDLKIASVASLIAGGVEFSSPDGRAGPAAAG
                     TTFTLFPDESSIGDSRIAAHIPYIIDFDGSVRGLQVGAPVEFRGIRTGTVTQIELIPD
                     FTSGKAPIRVYVDLEPQRFANSDPADTIEEQYERTKHLVSLGLRAQLKTGNLLTGDLF
                     IAMNLMPDAPPAEVEMIDGVPLIPSVPTDLEALTASISGLLDRLSSLPIEDIVQSLQR
                     SAATIENIVADPDVAAGIENLAAAGSDLRSLIAELEASTGPMARSAEAAVAQAQQTMQ
                     SVDAMLGQNSTLRYQLERVLQELTTAARSIRSFADMLDRDPSAIIRGRRE"
BASE COUNT         6772 a        14972 c        14265 g         6279 t
ORIGIN      
        1 tgggccgtcc tcgccagcgc cctcctcgcc gccacccgcc accgcctgcg cctgagaccg
       61 accacctggc gcctctgcca caccgccctc gccgccgtca tcgtcccggc cagcgtcgtc
      121 cacgcgctgc tgatcgaggg cacgatggga aagctctcga aagctgcgat ctgcgccctg
      181 gtcgtcgccg cggcggcgaa ggtcatcgtc gatcgacggg cttgggtcgt gctactgcgc
      241 cataatgttc aaggttgagg tctccggaca tggccaagct cgtgttcggc atgaacgtgt
      301 ccctcgatgg ctatgtcgac cacatggcgt tcgggcccgg cccgacgctc ttccgccact
      361 tcatcgaaga agcgaaggca cagtcgggca gcgtctacgg gcgccggatg tacgaaatca
      421 tgcgctactg ggacgacgat catcccgagt gggatacgga gcgtcaggcc tttgcggccg
      481 catggcgcaa gcagccgaaa tgggtcgcct cgcactcgct gaagtcagtc ggccccaacg
      541 ccacgcttgt cagggatgat ttcgagggcg ccatccgcag gctgaagacc gagcgtgacg
      601 gcgagatcga ggtcgccggc ccgaacctgg cgcaaagcct caccgagctc ggcctgatcg
      661 acgaataccg gatctacctg caccccgtcg tgctcggcaa aggcaagccc tacttcgccg
      721 gcccccgacc gccgctccgc ctcatgagct gtgaccggat cgacaaggac gtggtcaggc
      781 tgagctacgt gcctgcctga tccggcgacc gccccccctc acaccgccag ctccaccgtc
      841 accggcacgt ggtcgctcgg catctcccag ccgcgcgcct cgcgccggac atcggcatga
      901 cggagcttcg gcgccaaggc gggcgtgagc cagatgtggt cgagccggcg gccgcggtcc
      961 gaggcgttcc agtcgcggtt gcggtagctc caccaggaga agagcttctc ctcgggcggg
     1021 atgacggcgc ggacggcatc gacccagccg gcgctcgcct gcagccggcc gagcttctcg
     1081 acctcgacgg gcgtgtggct gacgacggaa agcagctgct tgtggctcca gacatccgtc
     1141 tccagcgggg cgatgttgag atcgccgagc aggatggcgc ggcggctctc gtagtgattg
     1201 gtggcgaacc agtccgaaag ctcgtccagc atggcgagct tgtggcggaa cttcgggttc
     1261 agctcggggt cggggatgtc gccgccggcc gggatgtaga gattgtgcag ctcgatgccg
     1321 ccggcgagcg tgcagaccgc atgcctcgaa tcgtcgatgc cgcaccattt gtggctgtgg
     1381 caggcatcga gcggcagctt cgcgagcacg gcgaccccgt gatagccctt ctggccgtgg
     1441 atgtggcagt gcggaaagcc caggtcgtgc acgagatcga gcgggaactt gccgtcctcg
     1501 accttggtct cctgcaggca gagcacgtcg ggatcgatct cggccacgat ccgccgcaag
     1561 gcagcctcgc gcagccgcac ggagttggcg ttccaggtgg tgatgcgcat cagtcttcca
     1621 gacaaaaagg tgctcagtcg ccgaccagca gcaggtcctc gggatggatg ccgatggtgc
     1681 agggtgtccc cggcggcggg tcgcccttgt cgatgcggtt ggtgttgtag cggctgcact
     1741 gcagccgcct gccgtccgcc gcggtgaggt agacgaccga gagatcgccg aaatagctga
     1801 tctggtcgat cctcgcctcg accagcacct cgcccgggtc gggatcgggc gaggagaaat
     1861 ccatgcgcat cttctcgggc cggacgatga gcgtgccggc ccggacgtcg gcagaggcgc
     1921 gctcgaccga aagctcgccc agcagcgggg cccgcacccg ggccatgccg ccctcgatgc
     1981 ccaggacctc gcactcgaag ctgttcgacg ccccgatgaa ctcggcgacg aagcgatgcg
     2041 tcgggtgctc gtagagctgc cgcggcggtg ctatctgcag gatcctgccc tccttcatca
     2101 ccgcgatgtc ggaggccatg ccgagcgctt cctcctggtc gtgggtgacg atgatgaagg
     2161 tgatgccgac ctcggtctgc agccgcacca gctcgagctg catctcctcc cggagcttct
     2221 tgtccagcgc cgagagcggc tcgtcgagga gcagcagctt cggccgcttg accagggccc
     2281 gggccagcgc cacgcgctgc cgctgccccc ccgagagctg gtccggcatg cgccccgcca
     2341 tgccgtcgag gcggaccatg gcgatcgcct cctcgacccg gggtccgatc tccgccttcg
     2401 gcacgcccgt gaccttcagg ccgtacgcga cgttctccgc caccgtcatg tgcgggaaga
     2461 cggcatagga ctggaagacc atgttgaccg ggcgccggtt gggcgggacg tccgccatgt
     2521 cctcgccgtc gatcaggacg cgccccgagg tcggatgcgc gaagccgccg agcatacgga
     2581 gcagcgtggt cttgccgcag cccgaggggc cgagcagggc gaagaaggcg ttccgcggaa
     2641 tggcgaggct caccccgtcc accgccttcg tgcggccgaa cgacttcgtg acgttctcga
     2701 aggtgacgat tggcgtgtcg gtcatgcgag cttcgccgcc tccttgtcgg gccgttgcaa
     2761 gcgaagtgcg atgacggtga ggatgaccgt gacgacgatc aggatggtcg aggcggcgtt
     2821 gacctcgggc gtcgccccga agcggaccat cgaatagacc tgcaccggga aggtcagcgt
     2881 gccgggcccg gccgtgaaga aggtgatgac gaagtcgtcg agggagagcg tcatcgccag
     2941 gaggccgccg gcgatcagcc cgggcttcat gaagggcacg gtgacgttcc agaaggtctg
     3001 ccactccgag gcacccaggt ccttcgacgc ctcctcgagc gagcgatcga acccggccat
     3061 gcgggcgcgc accacgacgc agacgaaggg aaagctgaag gccacgtggc cgatgatgat
     3121 cggcaagagg ctgaacggcc agccgaacgc cgccgagacc ccgacgcggt tgaagaagac
     3181 gaggagtgcc acgcccatgc agatctcggg aatgacgatg ggcagcgcca ccgccccgtc
     3241 gagcccgggc ttgccgggaa agcggaagcg ccagagggcg agcgccatca gtgccccgac
     3301 gatggtgctg atgaccgtcg agacgagggc gatgaccagg ctgttggtga aggcctcgat
     3361 cagcgacgcg ttggccgccg ccttgacgta gtagtcggtg gtgaagccct gccagacgat
     3421 gttgcgcttg ctgtcgttga agctgaagac gacgaggatg acgatgggcg catagaggaa
     3481 gacagcgacg gcaaggagcc agagccggag ccagagccgc cgcgtgtagt ccagcgggcc
     3541 gatcggctca cgccttgcca aggccgccct cccgcccggc gacccaggca cgcagcgcca
     3601 ggaccgcaaa ggtgacatag aggagaacga agctcatggc ggcccccaag ggccagttgt
     3661 tcgccgcctt gaactgccgc tcgatgacat tgccgatgag caggctgtcg gtgccgccca
     3721 ggagatcgga gatcaggaac gtgccgaggg cggggatgaa gacgaggatg atgcccgaga
     3781 cgatgccggg tctggccaag ggcacgatga tcgagaagaa ggtgcggatc tgcccggcgc
     3841 cgagatcgag gctggcctcc agcagcgatc ggtcgagccg gtcgagcgtc gcgtagagcg
     3901 gcagcaccat gaacggcagg tagacataga cgatgccgaa gatcaccgcc gtgttgttgt
     3961 agagcagctc gagcggcacg aagcgctcgc ccatcaccgc ctggtcgagc ccgaagggcg
     4021 ccagcaccac cgtgccgagc tgccagatgt actcgaggcc gaaattgacg aagcccttgg
     4081 tccgcagcac ggcgatcatc gcgtaggtgc ggatcaggat gttggtccag aacggcagga
     4141 tgatcagcag caggatcaag ggcttgagcc gcgccggcgc gaagctgatg gccagcgcca
     4201 ccggcaggcc gatgaccaag gccagcaccg tcgccaccgt ggcgacccag agcgagcggc
     4261 cgaagagccc gatatagacc ggatcgaaca cgcccgcgta gttggcgagc gtccaggtga
     4321 tgtcgatctc gaccggcccc gcccgctcgc cgaaggcgag gagccagatg agcgccaggg
     4381 gggcgatgaa gaagcagagc agccacaggg cacccggcag gacgaaggcc cagaacacgg
     4441 caggctgcgc gcgccagctc tccaagtctt tccctcggcc attcgctccg cgggctagtt
     4501 gcggcaccgc ccgcttgctt gtcaacggcg cttgcttttt gatcgccgga cgcccggcga
     4561 gcgggcaaca gggagcgtgc cgcatgccgt tcgagcagga cgtcgagatc gccgtggtcg
     4621 gtgccgggat caccggcctc gccatggcgc tggcgctggc ccgggccaat gccgggactg
     4681 gtgcggaagt agcgctcctc gccgccgccc cccttcccac gagccgggcc gaagcggcgg
     4741 acggccggac cgccgccctc ctcatgccgg cgatcgggct gctcacccgc ctcggcgtct
     4801 tcgaccccac gggcctcggc gccgagcccc tctatcgcct gcgcatcgtt ggcggtagcg
     4861 gcaagggtgc ggcggaaggc accgcgctca ccttcgatgc ggccgagctc ggcatggaag
     4921 ccttcggctg gaacatcccc aacgccacgc tggccgcccg cctcgccgcc gccgtcggcc
     4981 gcacggccgg catcgccgtc catgcgggcg cccccctcca aaacgtcacc gaggccggcg
     5041 accgcctgct gctggaggcc ggcgacaccc gcctcgccgc ccgcctcgtc atcggcgccg
     5101 acggacggga ttccatggtg cgggaaacgg ccggcattac caccacccag cgcgaccacg
     5161 gccagaccgc catcgtctgc cgcttcgccc acacgaagcc gcaccgcaac atctcgaccg
     5221 agttgcaccg ccagggggga cccttcacca tggtcccgct gcccggccgc aactccagcc
     5281 tcgtctgggt cgaccgcacg gccgagagcg aagcgcgcct cgatctcgac gacgacgcct
     5341 tccgcgaggc catggcgccc ctcgccgagc cctggctcgg cccgatcggc gccctctcca
     5401 ggcgtcacgc ctaccccatc cgcgcccgcc tggcgaaagc cctgaccgcc ccgcgccggg
     5461 cgctcgtcgg cgaggcggcg cacgtcatga gccccttggg cgcccaggga ctcaacctct
     5521 cgctgcgcga cgtcgccacc ctcgccggcc tcctcgccgc ccaccccggc gaccccggcg
     5581 atccggccct cctcgccgcc tacgccgagg cccgtgctgt cgacatcaag gcgcgcttct
     5641 ggggcatcga caccctcaac cacatggtca tgcagagcct cccgcccttc cgcctcgcca
     5701 gctcgctcgc cctcggcctc ctcgaccgcc tcccccccgc ccgccgcgcg ctgatgcagg
     5761 gcatgatgga aggtgtgccg gacggtcttt gagcaagaaa cccgagcagc actgccaatt
     5821 cgcatgagtt tgttgatttt tgcgaaacgg cagcccatat gatgtgccat ggaggagatc
     5881 accatggcgc tcgccacccc tcgtgcagcc gcaccggccg ggctcgacgc gctgggccag
     5941 gacccgaagc tgcaggccga cctgatgcgc ggcttcttcg gcatgatgcg cctctggcgt
     6001 gtcgagaacg ccgatgcgcg gcgcatcctc ggcgatccgg cggagcggac cttctatggc
     6061 tggaagggcg gcaaggtggg gcggctgccg gccgacaccg tccgccgcat cggctatgtc
     6121 gccggcatct tcaaggcgct gcagatcctc tacccggaca gccggcaggc cgaccagtgg
     6181 atcaggcagc cgaaccggca tttcggcggg cagacgccgc tcgcccgcat ggccgccggc
     6241 gacgtcaccg acctcgccgc cgtccgggcc tatctcgatg ccgcccgcgc cccctggtcc
     6301 tgaggcccct gatcctgatc ctgaggccga gccggcgctc gtccgggtcc gctgggacca
     6361 tgcgacccgc atcatcgcct cgcgctaccc gccgatcgac ctcttcgagc gggtcgacgg
     6421 cgacccggcc gtctgggagg cgctgatcgc ggcggagatg ctgaccaacc cgcgcatccg
     6481 cgacgggctg ggcgagatcg ccctggttgc acccgaggag cgcgtcgccg gccccggcgc
     6541 cacctacgtc atggcctcct tcacccacct caacccgaag ggctcgcgct tcagcgacgg
     6601 cagctacggc gtctattatg ccgccatcga cttccccacg gcgcgcgccg agacgattta
     6661 ccatttcgag cgcttcgccc gggacagcgg cgacgctgtc cgcgacaccg acatgcgcgt
     6721 cgttgtcggc aaggtcgacg agcgcttcca cgacatcgcc agcctccccg ggccccgccg
     6781 caccgcggtc ctggcgcccg acgactacac gtcgagtcaa gctctcggcc ggaggttacg
     6841 cgaggccggc agcaacggcg tcgtctggcc gagcgtgcgc cacgccgctg gcacctgcct
     6901 cggcgccttc cggccaaagg ccgtccagcc gcccgtgcag gagcgccaca tccgctacca
     6961 ctatgacggc cgacgggtca gcgcctggtt cgactacgcc accagcgcct ggcacgccct
     7021 ctgagcggcg gcgacgcccc tacggcaccg ccgtcagcgc ccccttgccc tcgaaccagc
     7081 tcaccaggtt ggcgaccacc gtgtcgccca tcgccgccat ggtctcctcg gtcgagctgg
     7141 cgatgtgcgg ggtgaggacg acattgtcga ggccgagcag gccttccggc acccgtggct
     7201 cgtcgtcgaa gacgtcgagc ccggcggcac cgagcccgcc cgaggccaga agctccacca
     7261 tggccggctc gtcgaccagc cagccccggg cgatgttgac gaagatgccg cgcgggccga
     7321 gcgcctcgag gatctgccgg ctgatgatcg gcgccgccgt gcccctcgcc gccccggcgg
     7381 cgcagaggaa gaggatgtcg ctctcgctgg cgagggtgac ggcatccgcc acgcggcgat
     7441 acggcacgtc gcgcgccacg gggtcgacat aggcgatctc gcacttgaag ccctcgaggc
     7501 ggctcgccac ctcgctgccg atgcggccga ggccgatcag ccccgccttc atgcccgtca
     7561 gcttgcgccc cagcacgagg cgccccttca gccagtcgcc attgcgcacg aaccggtcgc
     7621 catgggcgat cctccggcag gcggcgagcg ccagcgccag ggcgagatcg gccacgtcgt
     7681 catagaggac cggcgtgatg gtgatggcga tgcccttctc gcgggcgagg gcgagatcgg
     7741 tcatctcgag gccgacgccg ttgatggcgc agatctcgac gttgggcatg cggtccatgt
     7801 gctggcgctg caggccggcc atgccgctgc ccaccgcgcc gcgaaaccgc gccccgtgct
     7861 ggtcgagata ggccagcggg tccggctcgg catggacgtt gtggcacttg aagcgcgcct
     7921 cgagggcggc caccagggcc ggtcgctgcg ggccgatgat ggcgatctcg atatcggagc
     7981 gggcgggtcc gccggctgac gtcatgaatt tctctcccgt gctcagggcg cgcgcctcgg
     8041 cacggcctcc cgctgccaga tatgccgccg gctgcgcgtt cacgaaaggg aaaaggtgcg
     8101 tcgccccgag cccctggccc ggccggggct caggcggcga gcacccgggt cagcgcgttc
     8161 tcgtagaggc tctccaccgc ctcgcccttg tagatcgaga cctcgcactt atcgagcacc
     8221 gcgtccggcg ggtacatgga cgggttctcg aggtcctccg ccgggatata ggccttggcc
     8281 gcctcgttcg ggctcggaaa gcggacatag gtggcgatgg cgccgaagac ctcggcgtcg
     8341 agattgaagt tgatccactc gagcgccgcg tcggggttcg ggccgcctct cgggatcgcc
     8401 atgtcgtcct cccagagcag gccgccttcc ttcggcacca cgtaggccag gtcgtcgtcc
     8461 tcctccatca cctgcaggat gtcgccgttc cactccatgc agaggtcgac ctcgccggcg
     8521 atcagcaggt cctggccggt atcgggggtg aaggccttga tgttgggctt ggccctgatc
     8581 aggagatccg cagccgcgtc gatctctgcc gggtcggtgg tgttgagcga gtagccgaga
     8641 tagagcagcg ccatctggat caggttcttc tcgttgagca ccgagatccg gccggcgaac
     8701 tcgtcggatt cgaagagggc ggcccagctg tcgggcgagg cgacgctctg gcgatagccg
     8761 atgcccatgg tgccccagaa ataggggatg ctgaaccggc ggcccggatc gaagctcggg
     8821 ttctggaagc gcggcgccag attgccgaaa ttggtgagcc gggcgtggtc gagcggcagc
     8881 agcatgtcgg cgacgatcat ccgctcgacg aaatcgttcg agggatagac gacatcgtag
     8941 cccgggttcc cgtcgcgcag cttgccgaac atctcgtcgc tgctgccgta gaggtcgtag
     9001 cgcacgcgga tgccggtggc ggcggtgaag tcgtccagcg tgtcggggcc gatataggtg
     9061 tcccagttgt agacgttgag ctcgccaccc tgggccagcg gcagccgcgg cagggcgccg
     9121 gcgacggcgg ccacggcgag cccggcctgg atggcgcggc ggcgattgag catcggggcc
     9181 ggaaagggcg aaagtcggcg gggcatcctc gtctcctgca tcaaccggcc tctatcaacc
     9241 ggggagctat ctgccggcag tggttccgct cgcctcggca aatgtcaacg gctaccatgg
     9301 ccgcatggcg atggtttcat gaccgatcca gctttctggc cctggctgct cgcggccttc
     9361 cttctgggcc tcgtcctcgg catcggcgtc gccgccctcc tcggccgcgg gcaggcccgg
     9421 cggctgatgg gcgtcatggg cgaggcgcag cgccgccacg aggcggagac cgacgcgctc
     9481 ctcgacgggg tcaagctcgc cttccacgac atcggccgcg agctggccgg cgccagccgc
     9541 gacgatatgc tgcgcctcgc ccagacccag ctcgccggcg agcgccgcgc cctcggccac
     9601 acgctggagg cggaacgggg cgagctcgcc gcccgcatcg ccaccatgct cacccagctc
     9661 gagcgcaccc agggcctgat ccgcgagctc gagcgcgacc gcaccggcaa gttccaggcg
     9721 ctcgccaccc agctccgcgt cgccggcgac agtgccgccg agctcggccg gaccacccgc
     9781 cacctcgccc gcagcctgag cggggcgaag ctgcgcggcc agctcggcga gcgcctcgcc
     9841 gaggacgtgc tgaaggcaat gggcctcacc ccgggcatcc actacacccg ccaggccccg
     9901 ctcgacgccg agcgccgccc cgacttcctc ctcgccctcg gcgacggccg ccggctcgcc
     9961 atggacgtca agttcccgct cgacaactgg cgccgcgccg tcgaggccgc cgacgaggag
    10021 acccaggcgc ggctcgaggc gcagttcatg cgggacgtcc gcgcccatgt cgccgccgtg
    10081 gcgtcacgcg gctatgcggc gccggagctc ggcacgcacg acttcgcgct cctctnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggcc cgagcacgct ctacgccctg
    10261 ctcgcagcac tccgcatggc cgagggcgag gcccgcctgg cccgggccgg ccaggacctc
    10321 gcccgtgcca tcggcggctt ccgcctcgcc tggacccgct acgccgagcg cgcccagcgc
    10381 gtcgagcggc ggctcgagga cacgctcgac gagctgcgca agctctacgg cctgcgccgg
    10441 gcgacggtcg accgggccat ggcgaccgtc gaggcggtgc tcgacggtgc tccgccggag
    10501 ccggacacgg ccgatgagcc gcaggacgaa gctcagggtg cggcgaagag cgccagcttc
    10561 tcgggcggca gggcgctgac cagcgaatag gcatgcgccc cctcgtgcca gtcgaggcgg
    10621 gtcagcccgt cctcgaccgc gagcgccggc ggtgccaccg ccgcatcggc cgggttggcg
    10681 ccgggctcga tgaccagcag caccggcacc ccctccgcgg cgaagaccac cgccgcgcta
    10741 tccgggccgc ccggcagctc ggcgctgccg ccgacgagac cgaagccggc caccgccggc
    10801 agctcgatcg gcgccccgag ctcgcgatcg agccaggcgg cgagctcctt tggctcgccc
    10861 gtgcgcaggt cgagaacgag atcgcccgcc acataggctc ggtagagctg gatattgcgg
    10921 ctgccgccga gccagcccgt cgccaggtcg acgatggcgc cgaccgccgc gaccatcagc
    10981 gccgcacccg cgagggtctg cagccgccgc cgcaccggcc gccgcaccgc cgccgggcgc
    11041 tgccgcgcca gccccagccg caggcgatgc tggcccgcgc gcgccggccg cggcaccacc
    11101 ggaccggcct gggccacagg cgggtggaac aggatcggct gggactcgcg caccggctcg
    11161 gtcgtcatct cgagcccgag cggcgcccag agcgagacct cgtgctgcat ggcgcgcagc
    11221 cggcgctgca gatcggcctc gaacagcctg agctcgtcga cccgctcgct gcacggccga
    11281 cagagcgcca ggtgccggcg cagcgcatca cgccgcgcct cgccgagttg cccgtcgaga
    11341 cacgcctcca gataggattc caggtcagag cagtgcatgt tccgctcccc gacgcccccg
    11401 ctggcccagc cccgttcgac gcccaacgcc cgccggcggc agccacgcgg cctttggggc
    11461 cagtctcacc caaaccgggt gccgacgcaa tccccgcgca tgcgactgtt cgcggggcgg
    11521 cactatcggg tggcacgggc cagccgatgc gccgggccgc tttttcagtt gccttcgctc
    11581 gccacgccgc caaactgacc ggccaggcaa gggcaatgaa caagggagac aggcatggga
    11641 gcggcaccaa cgatcaggaa cgtggccctc gtcgggccgg ccggttccgg caagacgacg
    11701 ctgctcgaaa gcctgctctt cgtcagcggc gccgtgccgc gcaagggccg ggtggcggac
    11761 ggcagcacca tcggcgaccc gagccccgag gcccgcgccc ggcagatgag caccgaggtc
    11821 accgccgccg ccttcggcca tggcgaccac cgcttcacca tcctcgactg cccgggctcg
    11881 gtcgagttcg ccgccgaggc cagggccgcg ctgctcggca tcgacctcgc cgtggtcgtg
    11941 gtcgagcccg tggccgagcg gatgagcgcc gtcgccccgc tcctccacca cctggattcc
    12001 gccggcctgc cgcatgtcgt cttcatcaac aagatggacc gctcggaggt ccgctaccgc
    12061 gacctgctcg acgccctccg ccaggtcagc acgcgacccg tggtgccgca ccagtacgcc
    12121 atcggccggg gcgaggagct ggtcggctac atcgacctcg tgaccgagca ggcctacgcc
    12181 tacaaggacg gcgccccctc cgaccgcatc gacctccccg acgactaccg cgagcgcgag
    12241 caggaggccc gcgccgagat gctcgagacc ctcgccgact tcgacgacga cctcatggag
    12301 aagctgctcg aggaccagga gccctcgacc gaggagatcc tcaaggacct gcagaagacc
    12361 ctgaaggccg accaggtggt gcccgtcttc atgggcgtcg ccgagaagga catgggtgtg
    12421 cgccgcttcc tcgaggcgct cgaggccgag gccccggagc cggccgagcg agccacccgg
    12481 ctcggcctcg acccggacgg ccccacgatc gtccagatcc tcaagaacta ccaccagccg
    12541 cacgccggca agctctcgct ggcccgcgtc tggagcggga ccgtcaagga cggcatgcag
    12601 ctcggcggca tgcgcgtcgg cggcgtctac cggctcttcg gcgcccagca ggaagccgcc
    12661 ggcgaagccg ccgcgggcga gatcgtggca ttggcacgga tggacgaggc gaagcccggc
    12721 gccgtcctcg gcaatggcgg ggcggacgag gcggcggcca ccctgcccgc cctcgacacc
    12781 gggcagccca tgttcgcctt cgccgtcacc gccgccaacc gcaacgacga ggtcaagctc
    12841 tccgccgcct tccacaagct ctgcgacgag gatccgtccc tcgccctcga gcacgacgcc
    12901 gagaaccacc agaccctcct ctggggccac ggcgagatcc acctcaaggt cgccctcgac
    12961 cggctcaagg gcaagtacca cgtcgaggtc gagccccaca cgccccggac cggctacaag
    13021 gaaaccatca agaagggcac ctcgtcccac ggccgccaca agaagcagtc cggcggccac
    13081 ggccagttcg gcgacgtcaa gatcgacatc cgccccctgc cgcgcggcga gggcttcgcc
    13141 ttcaccaaca aggtcgtcgg cggctccatc cccaagcagt acatccccgc cgtcgaggcc
    13201 ggggcaaagg aattcatggc caagggtccg ctcggcttcc ccgtcgtcga cgtcggcgtc
    13261 accctcaatg acggccagtt ccacaccgtc gacagcaacg agctcagctt caagatggcc
    13321 accgccctcg ccctcaagga gggcctgccc aactgcagcc ccgtcctcct cgagcccgtc
    13381 ctcgccgtca ccatctccat cccgaccgac gccacctcca aggccctcca gctcatcagc
    13441 cagaaacgcg gccagatcct gggctacgaa gccaaggccg gctggtccgg ctgggacgag
    13501 atcaaggccc acctccccca gaacgaaacc cacgacctca tcgtctccct ccgctccatg
    13561 agccagggca caggcttctt cacctggtcc tacgaccacc tccaggaagt cccccagaaa
    13621 ctcgccgaaa ccctggcagc gcaagccaag gaggatgcgg cggcctgacg gggacgggaa
    13681 agaaggcagg gaggcgagcc tccctgcacc caccgttact tttgcggcgg aagatggtag
    13741 ggtattgctg tgccgcagat cgagtttgcg tgggatggca accgcaccga gatcagaaga
    13801 cgacgacatg cccgccgaga tcgacttctc gaagggcacg cgcggcaagt tcgcgaagcc
    13861 aggcacgacc ctgaagctgc ccgtctatct cgacgccgac gtggaagcct acctcaccgc
    13921 acgggcttca gcccgcggcg tcgacgtcga ccagctcgtc aatgagctcc tcaagaagga
    13981 catcgagctc atcgaggcgg cgggctgacg gagaagcacc aggagcgtca accgtgacca
    14041 acgaaagcct gcccgtcgaa gcgcccatga agaacccgcc ccatcccggc cgcatcgtcc
    14101 gccaggactg catcgaagcc ctcggcctga ccatcggcga agccgccgaa gcccccggcg
    14161 tcactcgcca aaccgtcgac cgactggtcc acgagcgcgg cggcgtctcg cccgagatgg
    14221 ccctgcgtct cgccaaggcc ttcgcagcac cacggagatg tggctgcgcc tgcaggcagc
    14281 gttcaatttg gcagaagcgc gcaagaccaa ggtcgaccaa ctcgcgaaca tccatcatct
    14341 taacattgcg gacgatttcg cggacgtgct cgcgaataac attaagcgct attagaactt
    14401 tctctattta accatatggc cagagcagca cttggacacg aaatactaaa tgccataaaa
    14461 aacgaaatta ttgcaatatc aatctctgat acaaacattg atccaagaat agaaagtaac
    14521 actatccata atgttatgag tcctattttc tgttcattaa gcataatttt gaaaccgaga
    14581 aagtattttg caattgcggc accgattaca actccaacaa taccaccaac gacattgaat
    14641 gaaatcgcga atacgaccac aaataccgct aagactgtcg caatcagttt caatacaacc
    14701 tcccccagtc gccgccgaac cacatatgaa aaacgtccta atggggtcct aatgaggtca
    14761 ggatccttta gtccgatggc caactcccga aatacgtaac gcgacaccac tgccctgtct
    14821 gaactgaacc tgcacacact tccccatcat caaagctcgc cctcggccaa tgccttccgc
    14881 agcacccttt cgacgcgggc attgtaaccc ttgcccgtcg cccggacacc ttcgacgaca
    14941 tcggccgcga ggcgaaaccc ggtatgcacc tttggcaatt cgggtccacg acactctttt
    15001 aaccgcaccc ggcgggggtc tcggatccgg gctgttcggc gtgacacctg cccattttcc
    15061 gggccacgcc accggcccct ccggtcgttt ggagtggaca cgggcacgtt ccacggcaga
    15121 aaacgtcgaa aacacggaat gcccctcggg gcctcgtggt cgcattcgga tcattccgcc
    15181 aatgtcggtc gagccatgtc acctccgagc atccacgatc gcaagacctt cggccccggc
    15241 aagctgctga tccgcccggg cgatccgcct gaccaagcct atctcatcca gtccggccgg
    15301 gcgcgcgtgt tcgacactat cgacggcaag cgccgggagc tcgccacgat gggtcccggg
    15361 gaaatcgtcg gcgacatggc cctgatccgc aaatccgacc acctccacgg cgtcgaggcc
    15421 atcgactcgg tcgttgccgt cgtcatctcg atccagcgct tgcacaaggc gctcgacggc
    15481 gcggacccac tcctgaagac gatcctcgag ggcatgctcg cccgcatcga ccgcctgaac
    15541 gagcagaagc agcccggcga catctggctg gtctagagcc cggctcaccg aggagcgcca
    15601 ggcatcgacc gctgccggca atgcccggcg agtgtcaggc gccagacggc atcaccgatc
    15661 ccaagaaacg gtggggccaa cggggaggcc gtgcctccct gtccctttct tcccgacctc
    15721 cctcccccag cacgcttccc cgctggacgc ctctcccggc gctggctagc ttcccggcgg
    15781 ttccatgtcg gggcctaaaa caggagggaa tgtcatggaa ccggttcatg tgatggcggt
    15841 gatcgaggcg aagcccggca agcgggcgga gctgctggcg gcgttcaagg cgaacgtgcc
    15901 ggcggtgcat gccgaggacg gctgcatcgt ctacgaggcg acggtggaca ccgaggcggc
    15961 agggcccatc cagaatgcga cgttcggggc ggacacgttc gtcgtggtgg agaagtgggc
    16021 gagcctcgaa gccctcgagg cgcatgcggc ggccccgcac atgaagagct acggcgagcg
    16081 ggtgaaggac ctcatcgccg agcgcacgat ctacgtgctg tcgccggcct gagcccgctg
    16141 ccgatcgccg gttgccggcc gggctgcggc ggccggtgac gggcgggacc gtctgcgatg
    16201 tatcagaacg cggcgctcct cgccctcctc agcttcctct acgtcctgat atcggggcgg
    16261 gtcgagcggt cgtggctgag cggccccatc ctcttcgtgg cgggcggctt tctcctgggc
    16321 cccggcgggc tggctgtgct gtcgctcgag atgaccgccg aggggctgcg gctcctcgcc
    16381 gagctgacgc tcgccatggt gctcttcagc gacgccgcca cggccgatct cgcccgcatc
    16441 cgccggagcc ggggcctgcc ggagcggctg ctgctcatcg gcctgccgct ggccatcctg
    16501 gccggcatgg gtgtcgcctg gctcatcttc cccggcatcg gcctcgtcga gatggcgctg
    16561 ctcgccgcca tcctggcccc caccgatgcc gccctcggcg agccggtggt ggccaaccgc
    16621 gaggtgccgc cggatcttcg ggcgacgctc agcttcgaga gcgggctcaa tgacggcatc
    16681 tgcgtgccgg tcgtcgtcct gctcgcgggc ttcgccatcg gcaccgagat cgagggccag
    16741 cccgcagcgc atgtgctccg ggtcatcgtc gaggagctcg gcatcggcct tgccaccggc
    16801 gccgtgctcg ccggcgcggc agccgccctc ctgcgcctcg cccatgcgcg caactggatc
    16861 gccgatggct ggcgcgacct ggcgctggtc gccgtcgcga tcgcctgctt cacgaccgcg
    16921 caggcgctgg gcggcagcgg cttcatcgcc tgcttcgtgg gcgggctggt cgtcaggctg
    16981 ccgcggccgc gccggcacga gctgctgcgc ggcacgcata tcgtcggcga cggcctcgcg
    17041 ctgctgacct gggtcgcctt cggtgccgcc atcgtctggc aggtggccga ccgggtcacc
    17101 ctgccggtcg tcctctatgc gctgctcagc ctgaccgtcg tccgcatgct gcccgtctat
    17161 ctctgcctga tgggctcgaa gctggcgccg cgcgaccggc tcttcgtcgg ctggttcggc
    17221 ccgcgcggcc tcgccagcat cgtcttcgcg ctgctcatcc tcgacaccgg cctgccgcat
    17281 ctggagatgc tcgaggcggt gatcgcctgc accgtcctct tgagcgtcct cgcccacggc
    17341 atgaccgcca acccgctcgt ccggctctgc gcccggcgct ggcgtcgaaa cccggggacc
    17401 gccaccagcc agccataacg aatcgtgatg gcccccggcc ggtcggcccg ccgctgtgtc
    17461 gtattccgcg acagcgggcc tcgggttggc gcatctggcg cgacaggaac ccgggaatgt
    17521 tgcagaatct gcaacagcgg accgccgatt gtcgcgccag atgcgccatt acggttcgtt
    17581 atgtgtcatc tattgcgaca tttcgagcca tcgaacccgg gaacgaaccc gccaaacgcg
    17641 gaacgaaccc gcggaatcga cgaacgaacc caagcgtcga gcaaacgaac ccgagccctc
    17701 cggcggctcg tttggctcta tccctcagat ggttgccgtg aatacctgcc atcgaagccg
    17761 aaaatcgaac ccgaatgcga acccaccgaa tcgcgatttg ccgagcgacc ccacccgcct
    17821 tgacaatcca ggcgaacaag gctattattc ctacaatcgc caaaaagccg gatgggcgtg
    17881 ggaaggcccg gccttcccac attcctgtcc ttcgccttca ggccagcggc ttcggcgcct
    17941 cgacgccgta tgtgcccgcc acctcggtga gcttcgtcat gatgcccgga tagagctcca
    18001 ctccgtcggc cagcgccttg cgcttcctgg cgaggccatt ctcgccgggc aggcggacgc
    18061 gctcgacgcc gggccggggc gggttggcgc ggcaggcgcc ggcgagccag ccggtctggc
    18121 ggttgaaggc gtcgctcccg gcgaagcagg cagggtcgat cacctggacg gtgaccgagg
    18181 ccccccagcc ctcgcccggg tcggcacggc cgaagcccgg caggccctgg gtgagggcct
    18241 cgatcatcag ggcgaggcca tagcccttgt ggccgtggtc gagcccgccg accggcagga
    18301 tcgagccggg cgggctggct gtcagcacgt tcgggtcgtc ggtcgggttg ccctcgccgt
    18361 cgagcagcca gggatgcggc atgcgccggc cctcggcggc gtaacggccg gagaggccgt
    18421 tggtggtgat cgaggcgctg atgtcgacga ggatcgggtc gccgtcggtc gggatgccga
    18481 tggcgatcgg gtccggggtg tagagcggct ggcggccgcc gtagggggcg accgaggcga
    18541 ccgacgggtc ggaggaggag atgatcgcca tcatgccggc atcggtggcg cgcggcagga
    18601 aggcggcgag gcaggcgata tgatggctgc ggcggatggc gaccatggcg gtgccgagct
    18661 cgcgcgcccg gtcgatggcc acacccagcg ccttgtcggt gagccagacg ccgggcagcc
    18721 ggtcgccgtc ccaggtgagc gccgccggcc ggtcggccag cacctcgggc tcgccggtgg
    18781 tgcgcatggc gccggcctcg gcggcagcca ggtagggggc gaggagctgc aggccatgcg
    18841 tggtgtgccc cagcatgtcg gcctcgacca ggagacgggc ggtcaccgcc gccttctcgt
    18901 cgtcgaaccc ggccttgcgg cacagggcct cggcgaagcc ggtcagtgcg tcggcggtcc
    18961 agcgctcggc cggcatggcg ttctcccctt ccctcgtcgc cgcaggctta acgcggcatt
    19021 catcaatgcg tgcgatgctc gacccgttcg cccgccgggc tccggccggg ccacgccttt
    19081 ggaccgagcc cggacggtca tgcagatcag cgacctcatc cagcaggccc gccgcctgac
    19141 ccggcagaag cagccggcgg gggtcagcct cgtgcatctc gccgcgtcgc cctacatcat
    19201 ggccaacgct gcccgctacc attttttccg caacagcatc gcggagctgg cgaccgcgca
    19261 cgccgccgat ctgcacgatc tgggccggag ccggctctgc ctcgtggccg ccgacgccgc
    19321 cggcatcgcc catctggcgc tccgccgcct gctcgagctg gagaccgagg cctggaacga
    19381 tggccgctcg gtcgacgata tcctcgaggg gttccgtctg cccgatcagt acatggagct
    19441 acgcgagcgg ctcgacgtgc tcgagcgggc aggccggcac ggcgccgacg atggcgacgc
    19501 ggccgcccgg ccggtgctcg aggaccgcct cgccgggccg ctgacgccag cgctgctggc
    19561 acggatcgag gaccggctcg acggcatcga cctcgccccc ttcgtccgtc gccaggcggt
    19621 ctatcgccgc ggggaggaat gggaggtcgc ctacaccgag catttcacca gcctcgacgg
    19681 gctcaaggtg gcgctctttc ccgaggtcga gctgcgcccc gacgagccgc tcttcttcga
    19741 gctctgccgc catctcgatc ggctgatgct gctggcgctg ctcctgaacc ggccctggcg
    19801 gcagcagcgc atcgggctca atctcggcca cgccgcctgg gccaccgagg agttccgccg
    19861 gctgctcgac cgcctcgacg cggccgagcg gggccggctg acgatcgagc tgcactggat
    19921 ggacgcgctc cgcgaccagg cggcgggcgg tgccgccatg gccgagatgc gcgctgccgg
    19981 catcaggatc gccatcgacc ggatcggcat cgaaagcctg cccttgctca atctcgaccg
    20041 gcttgaggca gatcacctca agctcgtctt cgacaagggc cgcctgccgc tgctgctcga
    20101 gccggctcat ctgggcgccc tgcgccgctg ccgcacggac cgggtggtgc tcacccagtg
    20161 cgacgatccg ctggcgctga gcctcggcca gcagctcggc atcacccact accagggctg
    20221 gctgatcgac cgcctcgcgc gcgaggcggt gccggcggag gagcgccggg ttgcctgagc
    20281 gcggctcggc caaggccgac tgggccgaaa agccttggtt ggcctcgcgg cttctgcgac
    20341 ggccctcgcc ggcgccccca gagcgcgggg gcggccggta ttcagcgagg gcatggcgcc
    20401 gctccagggg ctcgtccggc cctcctgcgg ccgcgcgagg gtcgagccgc cgccgtcaga
    20461 cgcccgcgtc gttggcgttg gggaagaaga gctgctggcc gccgatcctg tagtcggcaa
    20521 tcgccgcctg accctcgtcc gagatcagcc agtcgatgaa ggcctggcca tcctcggcct
    20581 tgacgtgcgg gtgcctcgcc gggtcgacga ggatgacgcc atactggttg aagagctcgg
    20641 ggtcgccctc gaccagaatc tcgagcggcc cgcgattgct gaagttgagc caggtggcac
    20701 gatcggtcag cgtgtaggcg ggcagctcgg ccgaggtgtt gagcgtcggg cccatgccgg
    20761 agccgagctc gcggtaccag gtgcccgcgg gctcgatccc ggcctcctgc cagaaccgca
    20821 gctcggcctt gtgcgtgccg ctgtcgtcgc cacgcgaggc gaaggtggcg ccagcggcgg
    20881 cgatggcggc gagcgcctcg acgacgtcgt ccttgccctc gatgcccgcc gggtcgtcgg
    20941 cgggcccgac gatgacgaaa tcgttgtaca tcaccgggtg gcgctcgacg ccggcgcctt
    21001 cggcgacgaa cgcctcctcg gccgaccggg catggacgaa cacgacgtcg gcgtcgcccc
    21061 gcctgccggt ctccagcgcc tgccccgtgc cctgcgccac gacccgcacc tcgatgccgg
    21121 tcgccgcctc gaactgcggc aggatatggc cgaagaggcc cgactgctcg gtcgacgtgg
    21181 tcgaggccac ggtgatgaag cgctcctgcg ccagagccgg cagggccatg gcgcaggtgg
    21241 cggcgaaggc gacgaaagcc aatgatcgac ggttcatgtg agacctcacc agatgagctc
    21301 gcccgcgacg aaggcgcggg cctcggggga acgtggcgcg gagaagaagg cgccggccgg
    21361 ccggtgctcg atcagccggc cacgatgcag gaagacgacc tcgtcggcga agcggcgggc
    21421 ctggccgagg tcgtgggtga ccatgacgag cttcaccccg tcacgcgcca tgagctggag
    21481 caggagctcg atccgggcgg tggccgcggg gtcgagcgcc gccgtcggct cgtcgaggaa
    21541 gagcacctcg ggctcgaggg cggcggcgcg ggccagcgcc aggacctgct gctcgccgcc
    21601 cgagaggttc cgcgccggcc gctcggcaag atccgcgaga ccgaatcgcg cgagcacagc
    21661 cagggcccgc tcccggcggt cgtgccagcc gaggccggcc agcgccaggc catgcagcac
    21721 attggcccgg gcggatcgcc gcagcatcac cggccgctga aagaggaggg agtggcgccg
    21781 cgccggtccg tcggcatcgt tgtcggacca gagaacccga ccctgagtcg gtcgcaggag
    21841 gccgtggcag agtcgcagga gaaggctctt gccggcgccg ttggggccga gcacgaccgt
    21901 gcgccggccg gcctccagca ccaggtcgac atcgtcgagc agctgcttgc cggcaatggc
    21961 gaagccgagg ccttgcagcc gcagcggcag gatggaggcc ggtgccggcc ggctcgcctc
    22021 gcctgcccgc cggagctgta cggggcccgt cagcacggcg ctcatgggac actcgctccc
    22081 catcggtcga gacgggcgag gagataggcg agagcgttca gggtcaccgt caggccgagc
    22141 aggatcaggc cgagcgccag ggcgagctcg agggcgccac ggctggtctc cagcgcgatc
    22201 gccgtggtca tcgtgcgggt gtggccggca atgttgccgc cgacgatcag caccgccccc
    22261 acttcggcgc tggcccggcc gaacccggcg agcagcgcgg tgatgaggct gaagcggccg
    22321 tcccagagca gcgtcggcac ggcgcgcaag ggcgaggagc cgagcgagcg gagctgctcg
    22381 gcatactcgc cccagagatc ctggatcacc tgccgtgtca gcgagacgac gatcggcagg
    22441 atcagcacgg attgcgccac gaccatagca gtcggcgtga acaggaggcc gagggagccg
    22501 agcggcccgg agcgcgacag gacgagatag atgacgagcc cggcgacgac cggcggcagg
    22561 cccatgagcg cgttggcgag gacaatggcg acgccgcgcc cgggaaagcg ggccagcgcc
    22621 agtgctgcac ccaggggcag gccgatcagc gcggccaggg ccagcgccgt cagcgtgacg
    22681 cgcagcgaca gcagcacgat ctcgacgagc accggatcgg ccgcaccgat gagctgcagc
    22741 gccagggtga atgcgctcgt ttctgccatg tcgtttccct cggctccctg aaggatggac
    22801 aggaccgtag atttcgacaa ccatggaatt gccgcgttct attgcaggat tttgcgatga
    22861 tgaagctcct caccaccgcc gaatgcgccg cctaccttcg cctgaaggag cgcagtctct
    22921 acgagctggc ggcgcggcgg cagatcccct gcacgcgcgc caccggcaag ctgctgttcc
    22981 cgctgcatct gctcgatcgt tggctcgagg cccgcaccga ggggagccag gatccgggcg
    23041 tcgcttcgcc gctgatctac gccggctcca gcgatccgct gctggactgg gcactgcgcg
    23101 aatccggcgc cggcctcgcg gtgctcgctt cgggcagcct tgccgggctg ggtcagcttg
    23161 cggcgggtgg tgcgcgcctc gccggcctgc atctgccgcc catcgatgcg gccgatgcgc
    23221 tgacgcccga cgacggggca gccgcggtcg atccggcctc ctcgggcaat gtcgcggcgc
    23281 tgcgcgccgc cctgcccttc gccgatgtgg tggcgatccg ctgggccgtc cggcggcagg
    23341 ggctgctggt cgctcccggc aacccgcgcg gcctcgccga ggccaccgat ctcctcgagc
    23401 cgggcctgcg ctgcgccatc cgcccgcccg acagcggcag cggtgtgctg ctcagccggc
    23461 tgctcgaggc tgcgggcgct gcggcggtgc cgacggaccc gacgctgctc gcccgcaccg
    23521 aggccgatct ggccgcactg gtcgccgacg gccgggccga ttgcggcctc ggcatcgagg
    23581 ccgtcgcccg gcgtttcggc acgggcttcg tgccgctcgc caccgaggct ttcgatctcg
    23641 tcatgcgccg ccgcgactat ttcgaggcac cgctgcaacg gctcgtcgcc ttcgcgcgct
    23701 cgaccgtctt cgcggccgag gccgagcgcc tcggcggcta cgacctggaa tcggctggcc
    23761 gggtggcctg gaacggctga ggcgagccgc tcgacgcggg ccggcgctgg tcaagatggc
    23821 ggcttgcgtg tatcgcgagc ggcgtcagac agttgcgagg tggaaggtcg gggtgatgcg
    23881 ccaggtcgtt ctgttttggc tggtggtggc cggcgtgctg ttcgcgtccc cggcgagcac
    23941 cgtccacgcc cagagcgatg cgccaccacc ggcgggcgtg atcgagctgc agcgcctcct
    24001 cgccgagccg gacgtgcagg cctggttgcg ccaggcagtg ccacaagcag caccggcgcc
    24061 gagcgaggcc gaggcgatcg tcgacgagga agaggtctac ctcgaggagg ccctgcaacg
    24121 ctggtccgag catcgcagca ccatcaccga agccttgccg gagctgcccg gcgccatcgc
    24181 cgacgccctc ggccgcctct ccgccgaggc ggccgaccgg ggaacactcc tggttttgct
    24241 gctcgtcgtc gtgttcgtcg gcgcgggtct cttagccgaa tgggttttca gccggctgac
    24301 ccgcccgctg cgccgcggcg tgctgacggc cggcgaggag accatcaagg cccggctgca
    24361 gaagctggcg ctccgcctgg ccctggtgct cggcacggtg ctggtcttcg ccgcagccag
    24421 cctcggcgcc ttcctgctgc tcgagctgcc gccgctcctg cgacaggtcg ccggccgtgt
    24481 cctcttcgcg atcatcctct tccgcctggt cttctacctt tgccgcctca tgctggcgcc
    24541 gactgcacca caactgcggg tcatgccgat cgacgatgcc tgggcgagct tctggttccg
    24601 ccggctgacc gtgctcgcgg gcgtctacgt gttcggctgg gcggcggcga ccagcctgcg
    24661 cacactgggc gccggctatc cctattcctt catcatcgcc tatctcttcg gtctcgccct
    24721 cttcgccgtc gtggtgacga cgatatggca gcgacgcgcc catctggtgc gatccgggct
    24781 cggcgcccgc acgatcgccg ccatcgcctg gaccgtgctc gccctcgccg ttctcttgct
    24841 ttgggtcggc ggcgcccagc ggctgatgtg gctcctcctc gtcggctcgc tgctgccgtt
    24901 ggcgatccac gtcaccaacg cctcggtgaa caacctgctg cgcccggctg cggggcaggt
    24961 ggtcgtcgag ccggtgcccg gcacggaggc cttggccacg agccccgagg acgagccggt
    25021 cccggtcgcc gggccaccca gcgtcacggc ggcggcgatc gagcgcggcg cgcgcgccct
    25081 cctgatcgtg ggcgccgcct ggctcctgat ctgggcctgg gatctggacc tgcccgagct
    25141 gatgcgcggc gaaggcaatt ttgcgcccct cgcccgggcc ctgctcaaca tcgccctgat
    25201 cgtcatcatc ttcgacttcg tctggcattt ggccaagacg gcgatcgacg gctacgtccg
    25261 catcgcggcg acgacagcac ccgccaacgc cgacgaggcg cgccgccgcg cccgattgcg
    25321 caccctgcta ccgattgcca ggacggcgct tctggcggtc ctggcggtca ccgctgtctt
    25381 gatgattctc tcggcggtgg gggtgcagat cgcccccctc atcgccggcg ccggtgtcgt
    25441 cggcgtcgcc atcggcttcg gctcgcagca actggtcaag gatatcatct cgggcgtctt
    25501 ctatctgctc gacgacgcgt ttcgggtcgg cgagtacatc gtcgccggca gctacaaggg
    25561 cacggtcgag agcttctcgc tgcgctcgat caagctgcgc caccatcgag ggccgctcta
    25621 caccgtcccg ttcggcacac tgggcgcgat ccagaacatg agccgcgact gggtgatcga
    25681 caagctgtcc ttcaccgtgc cgttcaacgc cgacgtcgcc aagatcaaga aggtggtaaa
    25741 ggaggtgagc aagcagcttc tggcggatcc ggagctcggc ccgggcttca tcgagcccat
    25801 gaagtcgcag ggtgccgacc tgatcggcga ttcgggcatc gtcgtccgca tcaagtacat
    25861 ggcgaagccg ggcacgcagt tcccgatccg ccgccgcgcc aatgccatgc tcatgcaggc
    25921 gttcgccgag aacggcatcg agatggcctt cccgaccgtg cgcgtggccg gcgagggtga
    25981 cagcgcggca gcggccaagg cggcaattga cgccaaggct gcggccgaag ccgccgagta
    26041 gtgctccggc agcatgaata gccgactgac agtcgacgaa cccccaccca tggtcgcggt
    26101 cgagcccgcg tcaacagcaa ggatggtgcc tgtggtgcgt cttctcggcc tcgctctgct
    26161 cctgcccggc ctcgccatgg cgcagcagcc accaccagcg cccgcggtga cggtggttgc
    26221 cgtcgagtcc agcgacatca cgccctcggt ctcgttcacc gggcgcatcg aggcggtcga
    26281 caaggtcgac ctgattgccc gggtggacgg cttcctcgag gcacgaccgt tcaccgaggg
    26341 cgacatggtg cagaagggtg acctgctctt cgccatcgag aagggcccct acgaggcacg
    26401 gctcggcgag gtcgatgccg cgatcgcctc cgcccaggca tccttcaagc tcgcccagat
    26461 cgaggtcgac agacagacgg tcctcgtcca gcgggaggcc gccgcccagg cggtgctgga
    26521 ccgggccatc gccgaggccg accaggcgcg cgcggccgtc cagagtgccg aggccacgcg
    26581 ggccgatgca gagctcgatc tcggctatac cgacatcagg gcgccattcg acggccggat
    26641 cggtctggca tcggtgtcgg tgggcgccta tgtcggcctg gacagcggat ccctcgcgac
    26701 catcgtcagc caggacccga tatacgtgac ctttccggtc acccagcgtc agctgctcgc
    26761 gtttcgcgaa ggggctgcgg gcggcgacac atcgacgatc gccgtcaagg tccgcctcgc
    26821 caacggcaca ctctacgacc aggtcggcca gatcgatttc gtcgacgtcg aggtcgacac
    26881 gggcaccgac tcgatcacgg tgcgcgccgt cctcgccaat ccggattccc tgctgatcga
    26941 ccagcagctg gttacggccg tcgtcgagac ggccgccccc cagacggcgc tgctcgtgcc
    27001 gcagcaggcg gtgctgtccg accagggcgg tcgcttcgtt ctgctcgtcg acgccgacaa
    27061 caaggtcgag cagcggccga tcgaggtcgg ccaaacgcag ggcgccaact atgtggtcac
    27121 caagggcctc gaggagggcg atcaggtgat cacggacggc atccagcgcg tgcggccagg
    27181 catagtggtc gatccggggc cgccgcccga cgccgctccg gagccggcgg ccagccccga
    27241 cagcggaaat tgaacccatg atctccgccg tcttcgtcga ccggccacgg ctcgcgatgg
    27301 tcatctcgat cgtcatcacg ctggccgggc tcatcgccat gctggcaatt ccgaccgccc
    27361 agttcccgga catcgtgcca cctcaggtct cggtgacgac gacctatccg ggcgccggct
    27421 cggacgtcgt cgaggcgacc gtcggccagc cgatcgaggc caaggtcatc ggtgtggaga
    27481 acatgctcta catgaagtcg accagcggca gcgacggcag ctacacgctg accgtcagct
    27541 tcacggtcgg cagcgacgcg gacatcaaca ccgtcaatgt ccagaaccgc gtcgcccagg
    27601 cgctgtcgca gttgccgcag gaggtgcagc ggcagggcgt cacgacgaag aagaagtcgt
    27661 cggccttcct gcagatggtc agcctgtact cgcccgatga gagccgcgac gtcctcttcc
    27721 tctccaacta cgccaccatc aacatcatcg atcagatcaa gcgcattccg ggcgtcggcg
    27781 atgcctacct cttcggcgga ctcgactatg ccatgcgcat ctggatcgac gatcagcggc
    27841 tgaccgcgct cggcttgacc ccgaacgacg tcacccaggc gatccaggcg cagaacgtcc
    27901 aggccgcggt cggccgcatc ggcgcccagc cgatggccga tgccgagctg cagatcaata
    27961 tcaccacaaa gggccggctg accgacgtcg aggaattctc ggatatcgtc atccgcgcca
    28021 atccggacgg ctcgtatgtt cgggtgcgcg acgtcgccga ggtcggcctc ggcgccaaga
    28081 gccgtgacgt cgccgcccgc ttcaatggtg gcgacggtgc cgtcatcgcc atccagcagc
    28141 tgccgggctc gaacgccatc gaggccgccg ccggcgtccg cgagctgctc gaggagctca
    28201 agacgcgatt cccggccggc gtcgactacg ccatccccta cgatacgacg ctgttcgtgc
    28261 aggccagcat cgaggaggtg atccacacgc tcgtcgaggc cttcgtgctg gtcatcatcg
    28321 tcgtcttcct gttcctcggc aacgcccggg cgacgctgat cccgctgatc gccgtgccgg
    28381 tctcgctgat cggcaccttc gccgtcatgc tggcgctcgg cttctccgcc aacaccgtct
    28441 cgctgctcgc cctcgtgctg gcgatcggca tcgtggtcga cgacgccatc gtcgtcgtcg
    28501 aggcggtcga ggccaagctg gaggccaagc ccgagctctc ggtcgccgac gccaccaagg
    28561 aagcgatgag cgagatcacg gggcccatca tcgccatcac gctggtcctg ctctcggtct
    28621 tcgtgccggt cgccttcatc cccggcattt ccggccagct ctatcagcag ttcgcggtag
    28681 ccgtgtcggt gtcgatggtg atctcggcga tcaacgcgct gtcgctctcg ccggcgctct
    28741 gctcggtcct gctgagccac caccatggcc ccaagcgcgg gccgatgcgc tacgtcatgg
    28801 cagcgatcga caagacccgc gacggctacg ccaatatcgt cggccgcctc gtccgggtcg
    28861 ccgtcgtcag cctcgtcctg ctcgccggct tcatggtcgg caccggctgg ctggccggcg
    28921 tcgtgccgac cgggttcctg ccggacgagg atcagggcgc gctcttcatc gagatgcagt
    28981 tgcccgaggg tgcctcgctc aaccgcacca tcgccgcctc gcgaaaggtc gaggacgtgg
    29041 tgcgggacaa gccaggcgtc gccgacgtca ccgtggtcaa cggctacagc ttcatcgacg
    29101 gcctcgccaa gtcgaactcg gcctttttgg tcctgaccct ggccgacttc gccgagcgaa
    29161 ccgcgccgga gatgcgggtg gaccacctcc tgcccgacct gcggcgcgag ttcgcgagct
    29221 tccgcgaggc gctggtcatc cccttcaacc tgccaccgat catcggcctc ggcaccggcg
    29281 ccggcttcga gttccagttg caggacctgc agggcggcag ctcggccgac ctcgcctcgg
    29341 tcacccgcgg cctgatcgtc gccgccaatc aggacgaccg gctgaccggc gtctacacca
    29401 ccttctccgc cgccacgccg cagatctatc tcgacatcga ccgcgagcgg gtgcagaccc
    29461 tcggcatcga cgtcagcgac gtcttcaacg ccctgcagtc gaccctgggc ggctactacg
    29521 tcaacgactt caacctcttc ggccggacct ggcaggtcct cgtccagggc caggagcagg
    29581 atcgcgccgt cgtcgacgac atctaccgca tccacgtccg caacgatgcc ggccagatgg
    29641 tgccggtgcg cgccttcgcc gagccacggc tgacgctcgg gccgcagttc gtcacccgct
    29701 acaacaactt ccgcagcgta accgtggccg ggacgccgtc ggcgggacac agctctggcg
    29761 aggcgatcac ggccatggag gaggtcgccg ccgaaaccct gccggccggc tacggctacg
    29821 agtggaccgg cacggcgctg caggagatcg aggccgccgg ccagaccagc accatcctcg
    29881 ggctcgccgt cctcttcgcc tatctcttcc tggtcgccct ctacgagagc tggatgatcc
    29941 cggtgccggt cctgctctcg gtcacggtcg gtctcttcgc cgccatgctc ggcctcatgc
    30001 tgttcggcct cgacaacaac atctacgccc agatcggcat cgtcgtgctg atcgccctgg
    30061 ccgccaagaa cggcatcctg atcatcgagt tctccaagat gcggcgcgag gagggccatc
    30121 cgatcggcga ggcggcggtc atgggtgccc gcgagcggtt ccgtgccgtg atgatgacca
    30181 gcttcgcctt tattgccggc ctctacccgc tggtcaccgc caccggcgcc ggcgagctca
    30241 gccggcgcgg cgtcggcacc tccgtcttcg ccggcatgat cggtgcgtcc ctggtcggca
    30301 tcttcatgat cccgaccctc tatgtctgct tcgagtgggt gcgcgagaac agcacgcggc
    30361 tttttccatg gcgccacaag ccggccgcag agtcccacaa gccggcggag aaggccgcac
    30421 cttagggcgt ccagtcgatc cggtgcgtcg ccaggtaggc gtcgacggcg gcgccgaggg
    30481 tggggaagaa gctcgcatcg ccgaactggt ccagcagctc gaagcggcgc agcttgtcct
    30541 tgaccgggtc cttcagctcg gcgaagcgca gctcgatccc ctgctcccgc agccgcgtct
    30601 cgagctctgc cagcatgtcc gccgccgtca cgtcgacgct ggtgatcggc tcggccgtga
    30661 cgatgacctg ccgcaccggg cccgatgccg aggcgatcgc ctcggtcagg ttctcctgaa
    30721 agaactcggc attggcgaag aagagcggcg catcccagcg gaagatgacg agcccgggca
    30781 ccagccgcgc cttggggtac cgcttgaggt cgtggtagcc gcgcagaccg tcgacccggc
    30841 cgagcaccgc ccagtgcggc cgccagccat cccagaggaa ctcgatcacc gcggcgacaa
    30901 tggcgagccc gatgccctcg atcgggccga gcaaggcgac cgccgcgaag cacgccatcg
    30961 acagccagaa ctcccagcgc tggatgcggt agagccgacc gagatcggcg aactcgaaga
    31021 gaccgatggc agcggcgatc acgacggcgg cgagcgcgct cttcggcagg tcgctcagca
    31081 gccccggcgt cagcacgagc agcagcatga cgccgacggc accgatgacg ccggtgagct
    31141 gcgtgcgcgc acccgccgat tccgcgaccg gcgtgcgcga cgagctgctg ctgatcggga
    31201 agccctggaa gaaaccggtg gcgagattgg cggcacccag gccgatcatc tcctggttag
    31261 gatcgacgct ggtcttgagc cgggcggcat aggagcgcga cagcacgctg gtatcggcga
    31321 acgagaccag cgccacggca cagccaccga ccaccaccgg cacgatgtcg gctggcgtcg
    31381 gccacggcag ggcaaaggcc ggcaggccct gcggcagggc gccgagcacg atgacgtctg
    31441 cggtctcgcc gaggtcgagg acggcgacga ggacggtggc aaccatgacg ccgaggagga
    31501 tgccggagaa tcgcgtcccg tgcttgaggc agaggatcag caccagcacg ccgatgccga
    31561 tcgcggccgc cggcccgttg gcggcaccgt cgagcaccgc cccggcgatg gcgacgaggt
    31621 cgagcagcgg cccggtgctt tcgatcgaga cctgcaggag gctcggcagc tggctgatga
    31681 tcacggtgag cgcgataccg ttcatgtagc catagcggat cggcttggag agcagctcgg
    31741 tgacgaagcc gagccgcagc agcccggcga ggatgcagac gaggccggcc accacggcca
    31801 tcgccccgcc cagcgccacc gcgcggaccg ggtcgccgcc ggagagcggc aggatggtgg
    31861 cgaggatcag ggtcgctagc gcggagtcgg ggcccagcac caggatccgg ctcggcccga
    31921 agagggcata gacgaggagt gggacgatgg tcgcgtagag tccgcagatc cccggcagac
    31981 ccgaggccac ggcataggcg atgccgactg gcaccagcat ggccgcaagg acgaggccgg
    32041 cgacgaggtc atgcggcagc caggccagct cgtaatgccg cgccgtggcg atccccggca
    32101 gccagcgctg ccagcccttg gtcgccggcg ccgcgaccag tcctccgttc attccggccc
    32161 ccccgagctg cggcgccacc aactttgcgg cgtgaggaag aacgctaggc cgatgatcac
    32221 atagacggca aggagctggg cccctttccg ccagtccgag cggctgtcgc cggccacctg
    32281 ggcgatgacc agcactgcca ggagcaccac gagcacctgc cccttgccga agacgaggtc
    32341 caccggcgcc ggaacgatgc ccgggatcgc caggacgagc acaggtgcca cgaagaacgc
    32401 gatctgcacg ctcgagccga tggcgatcga gagcgagaga tccatccggt tctgcagcgc
    32461 cgccgtgatt gcggtcgcgt gctcggctgc attgccgagg atggcgatga cgaagacgcc
    32521 gatgaagatg ttgctgagac cgagatcgtg ggcgctcgac tcgatggctc ggtggctttg
    32581 tcgatagtgg cgggggccga cgggtgagcc gcttgccgca ccatcgatga aagctcttgc
    32641 ccggcacatg gggtggggcg agactcgcca gtgctccagg ccactctgtc cagctaccgg
    32701 atcatcacct tgcccgaccc gcgctcgctc gttccacccg accgcctgcg aagcctcgcc
    32761 cgacgccgcg acatgcccgg gttggttttt ctcggcctgc atctcgtgct gctcgggcta
    32821 accggtacgt tggtgatcct gatgctcgga agctggtggc tgtggccggc catggtcgtc
    32881 catggcatcg tcatcgtgca tctcttcgcc cccttccacg agacggcgca ttccagcgcc
    32941 ttccgaaacc gccggctgaa tacgacggtg ctctgggcga ccggcctcgt gctgggcctg
    33001 cccccgacct tcttccgact cgagcatgcg gcacaccacg cccacacctc cgatccgggc
    33061 aaggatccgg agatggtggc ggcgagcggg agcgttgcag gctacctctg gtatgcgacc
    33121 gccctgccct atttctgggc gctcttctcc tcgctcttcc gccatccctt tggtgcgttc
    33181 accgcggtcg agcaggcttt cattccggcg agccaacggc gccgggtgca gcgcgaggca
    33241 atttggatgt gggccgtcta tgcggcgatc gcctcggctt cgatcgccac gggatcgacc
    33301 ctgacgctca cctgctggct ggtgccgcgc gtcctgggcg agcccttcat gcggatcatc
    33361 cggatgagcg agcacggcgc ctgcccgctg gtgccggaca tgctggtcaa cacccgcacc
    33421 gtgctgaccc tgaggccgat ccagtggctc aactggaaca tggcgttcca cgccgagcac
    33481 catgccatcc cggccgtgcc gtttcatgcc ctgccggcgc tgcaccgcga tctcgggccg
    33541 catctggccg cgctcgagcg aggctatagc cgcacccaag ccgtgatcct cggcgccgcc
    33601 tggtcgacga gggagccgaa acgcgccgcc gcctgagcga tcgaaggcgg cgggctgaac
    33661 gagcgaggag gaacggaaac catgcaaaag gtcgatgtcg cggtgatcgg cgccggcacc
    33721 gccgggctca acgcgcgcag cgaggtggcc aggtgcggcg gcagcgtgct cctgatcgag
    33781 cacgggccct atggcaccac ctgcgcgcgc gtcggctgca tgccgtccaa gctgctggtc
    33841 gccgccgccg agcgcgccca agcggtgcgc gatgccggcg tcttcggcgt gcatgcggcg
    33901 ggcgggctcg agatcgacgg gcgggcggtc atggcgcgcg tgcagaagga gcgcgatcgt
    33961 tttgccaatg gcatggtccg ctcgacagag gccctgccgg aggaccagcg tctccgcggc
    34021 cacgctcgct tcgtcggccc caccagcctc gaggtcgagg gcgagcgcat cgaggccaac
    34081 gccgtggtca tcgccgccgg cagctcaccc tggctgccgc cgcccttcga cgaccacgcc
    34141 gaccgggtgc tgaccagcga caccatcttc gagctcgagg acctgccgga aagcctcgcg
    34201 gtcatcggca ccggcgtgat cgcgctcgag ctcggccagg ccatggcgca gctcggtgtc
    34261 gaggtcgcct tcttcaaccc cttcgacgat ctggggccgt tctctgaccc ggacctcatg
    34321 gaagccgccc gggccacgct cggcgccggg ctcgagctgc acctcggggc cgagatgcag
    34381 ggcgtcgagg cgactggccc gggccttcgg ctcacctggc ggacggccga cggcgagcgc
    34441 cacgctcgcc gcttcgccgc ggtgctggtc gccgccgggc gacggccgaa cctcgcaggt
    34501 ctgggcctcg aggcgaccgg cctccggctg gacgacaagg gccgccccgg gtggaaccgc
    34561 cggaccaccc agtgcggcga cctgccgatc ttcatggcgg gcgacattgc cggcggcacc
    34621 ggcctgctgc acgaggcggc ggacgagggg cggattgccg gcgccaatgc cctgctctac
    34681 cccgcggtgg aggcgcacaa tcgccgggcc ccgctcgcca tcgccttcac cgacccgcag
    34741 atggcgatcg tcggccagcg tttcgccgat ctggaccagg gcgaggtggc catcggtgcc
    34801 gtctccttcg ccaaccaggg acgggccacg gtgatggcca agaacgaggg gctggtgcgc
    34861 ctctacgcaa ggcgcggctg cggcacgttc ctcggcgcgg agatgctcgg gccgcaggtc
    34921 gagcacatgg cgcatctcct cgcctggtcg tgccagcagg agctgagcgt gccgcgcatg
    34981 ctggagatgc cgttctacca tccggtgctg gaggaaggcc tcaggacggc ccttcgggcg
    35041 ctggcgaagg agctcgacat ggtgagcacc aggcgctgcg aggatgcggc cgagcagccg
    35101 tgctcctgac ctccgctcag gccgcggcga agccccgccg atcctgtcgg ccccagtggg
    35161 cggcaccgcg ccagtactgc cagtggccct ggatgcgcca gaaattggcg agctggcggt
    35221 agccgaaatt ctcgatgatg gcggccgccg tgagcacgac gagatcgcgg gcccgcggca
    35281 cgcgccgtcg ctcgaactcc tcgagcacca gcgacgccac ggagatgaag acgccgaagg
    35341 cgaaggtgag cgccaggtag gccaggaaga acggggtgtt gatggttccg gcgagccaga
    35401 agaagggcag caggagataa cccagcacct cgaccagcgg cgtgaccacg tccagcaaca
    35461 ggatgtggcc gaggccgagc cggcccacgc ggccatactt gcggccgacc agcatgtcct
    35521 tgtggcggaa gaaggtctcg agggcaccac gttgccagcg ctgacgctgg cgagcgagct
    35581 cgccgaggcg ctccggcact tgcgtccagc agacgggctc cggcacgaac tcgattcgat
    35641 agtcgcggcc ggcattgcac atccggcgat gcaggcggac cacgagctcg agatcctcgc
    35701 ccacggtctt cgtgctgtag ccgccgagct cgatgacaac atcgcggcga aagaggccga
    35761 aggcgccgga gacgatcatc aggcagccga tgcgtcccca ggcgagcctg gccatcatga
    35821 aggcgcgcag atactcgacc gtctgcagga gtggcagcat ccggcgcggc agggccacgg
    35881 cgcgcacccg gccggcccgc acgccgcagc cattggccac acgcaccgtg ccaccaacgg
    35941 cgaccacgcg ctccgggtcc tcgacaaagg gctggaccac gcggagcaga ccgtcggcct
    36001 cgatcaggct gtcggcgtcg acggcgcaga agagctctgt cgtaacctgt ccgagcccgg
    36061 cattcagggc gtcggccttg ccgctattga ccttgtcgag caccacgagg ttggcatggc
    36121 gggtcgatct gtagagcccc ctggcgggag cgtgcggcag ggtcgagacg tgctccagcg
    36181 gggctggctc cagcccgaac tcgcgcatga gcacgtccag cgtcgcgtcg gtcgagccgt
    36241 cgttcacgac gaccacgcga aagtccggat actcgagcgc caggagcgag cgcagcgaat
    36301 cggcgatcgt ctcggcctcg ttgtaggccg gaacgatgac gctgaccggt ggcgccccgt
    36361 cgacgaggcc attccagagg gcgcggctct gatgcacgcg cggccggccg agcatcacgg
    36421 aaaccgcggt caggaggaga acgaggtaca gcaggttctg cgccatgctg acgacgatga
    36481 cgaggacggc cgtcgtctgg gccagaagat cgaagaacgg cgggaacggg atcatgccgg
    36541 gacggctcca tcggcggtgg cagccctgag acgggcgagg agtttctcgg cattggcggc
    36601 gatgtcgacg accggatcca gggcgaggct cgagaccagc tcggcatcac gagcccggcc
    36661 gcgtcgctcc agcacaccga gtgctgcccg ccgggattcg gcctctggag ccccacccag
    36721 ggcacgcgcc aagcgcagca cggccggatg attgagccgg tcgagggtga ctagtgccgt
    36781 ccggtcgacg gcggcaccat gtcggccgag cagggcctcg atctcggtgg cggcggccgt
    36841 atggccggca tcgccgagcg cctcgagcag ccggatcagg agggccgcat cgacgtcggc
    36901 acgggctagg caagtgacga ccgtctcggg cgcgttggcg gccagccgat gccagaaccg
    36961 cgcggcatgg cgaccgcggg aagcgggatc gacgagcagt cgctcgacca gccacgcagc
    37021 gagcgcggtg tcctgggcga gggcctccgc cgcggccagc cgatgccggg catcgccctg
    37081 cgcgagcatg gtggtgagcg cgccccgcgc agggtccgtg gcgagcaggc cgaggcagcg
    37141 cacggcatcc agccggcgga gggtggtggc cgagcgcaac cggcgcaacc agtaccgttc
    37201 gatcccggcg ccgacggcga aggcggcgag cctcgctttg gcgtcacccc ggacgatctg
    37261 ggcgagctcg tcgacgagcc gcgcgatctc ggccaccggc aggccggcgg gtgctgcaac
    37321 cgggtcgccc gacggccggt tgagactggc gagcagggcc ctgcgtaggc cctcgcgggc
    37381 cggccggtgg cgcgccgccg cccattcctc gaggagccgg cgcagcacga ggctcgcgat
    37441 caggatgagc ccgatggcga cgagcaccag cgtcgcctgc cagatcagcc gcatcaggaa
    37501 gtcgagggac ccgaggccga gcatcagaac cgcacccgga aaccgagcgc gaccgtggtc
    37561 tgccggacac cgttgtcgcg atcctccagc gccaggctca gggccacgcc gagccggtcg
    37621 gtgagccagt ggtcgagccc gacgaaatag ctgtcggtgg tgcccatgcc ttgggtgctg
    37681 ttgtcatacg actggccgaa gccgccgaaa acccggctgt ccggcgtgag ctgccagtcg
    37741 agccggagcc cgtaattggg gtcgaagctg ccattggtgc cgaagctgcc gccggccttg
    37801 gcggtgagcc agacgcggcc ctcccagcca tactgcacga gcccgagatc gaacccctga
    37861 acaagcccgt cctcgtagtc ggcgagcgat ccttcgacga aaccgacgcc gatgccgagc
    37921 cgaccgaccc ggccggtcag atcgaagagc tcgcgatcga gcccgccgcg cagcttccag
    37981 gatggcagga agttggcggc cggggtgact gcggcaccaa gaaaatagcc gctccgcgcg
    38041 tcgagcctcc cgttccagac gaccgccgcc tgaatgtcgg tgctgccgta gcggttggcc
    38101 acggcgaggc cgccggtaag ctggcgcgaa tcgtcgatgc gccaggcgag gctgatcccg
    38161 ccttcgcgcc agtcgtcgct gtcgtcgttg aagcgactga acgagagatc ggtcgagagc
    38221 tcgaaacggc gctgctccgc ggccagggtg tcgagccgct cgcgtgccac gggtgccgtc
    38281 tcgggcacca ccagcgcccg ctcgtagttg agctgggcga cgttgatgac accttcgctt
    38341 cgggcgacgt cgccgaggcc gagccaggcg tcgccggagt cgggctccag caccgcggcg
    38401 cgggcgaaag catccctcgc cacctcgggc tcgcgggcgg cgagcgccgt ccggccgagc
    38461 agaagctgcg catcgaagcg agccacctcg ggcgtcacag cggatcgcag caccgccagc
    38521 gccgcgtcgt gccgctcggc atagccatgc acgcgggcct gcatcagccg aatatcgaga
    38581 tagtcgggcg ccagcctcgc cgcttccgcc agcgtctcga gcgcactccg gtcgtcgccg
    38641 agaaaggcca ggatctgccc cttgatcagg agcaggtcgg catcgtccgg ggtcttggcc
    38701 aagagggcgt ccaccacctc cagcgcctgc tcgaactcgc catcggcgcg gagctgcagc
    38761 agcgtctcgc gactgacgct ggggaccgcc cccatggccg gcgcgctcgt gagcggcgac
    38821 agcagcaggc cgccgagcag caccagtgcc ggccagcccg atttcgacgg aaggcgtaag
    38881 tcgattctca tgcggggacg cgcagcgcct tctggatgcg catcgccagc tcctccggca
    38941 tgaacggctt gaccagatag tcgctggccc cctcggccag cccgccgaca acgtcccgtt
    39001 cctgcttcct ggcgctcagc atgatgaccg gcagatcgag gcccgcctcc cgccgtaacc
    39061 ggcgaaggac atcgaggccg tcggccccag gcatcatgcc gtcgagcacc atcacgtccg
    39121 gccgcacgcg aacggcgatc tcgaacgcgt cagcgccgtc ctcggcggtc agcacggtgt
    39181 gcccgtggcc ttcgagggta aaggtgacga gctcgaccag aagctcgtcg tcgtcgacga
    39241 ccagaacgcg cgccatgctg cctccaacca gaaatcggcc cgaggctcgt cgatgacgcc
    39301 cagcggccgg tgctcacggc cagggattag agactagtaa ggttaacagg cggctaacgc
    39361 ccgtgttggc tctgcgtcag gccacggccg tcagcagcgg ccggccggcg aaatgcgccc
    39421 gcaggttgtc gaccacgagc tgggccatgg catcccgtgt ctcgtcggtc gcgctcgcgt
    39481 ggtgcggagt caggacgacg ttctccatct tcaggagcgc ctccggcacg cgcggctcgt
    39541 cggcgaagac atcgagcccc gcacccctta ttcgcccctc ctcgagggct ttgaccaagg
    39601 ccggctcgtc gaccaccgaa ccgcgggaga tattgacgag ccagccctgc gggccgagcg
    39661 cctcgatcac cgactcgttg accagatggt gcgtcgccgg cccgccggga caggcaagga
    39721 ccaatacgtc ggcccaacgg gcgagctcga ccggtgtcgc gaaatagggc aggtcgacgt
    39781 ccggccgccg gttccggttg tggtaggccg ggatcatctc gagcgcctgg gcccgcttcg
    39841 ccaactcctg gccgatccgc cccatgccga cgatccccag cttcttgccg gtgacgcagg
    39901 tgcccatcgg catcgcaccc tcgagccagg caccgcggcg gacgaagcgg tcggcctcgg
    39961 ccagccggcg gagcgtcatc aggatgaggc cgatgcccat gtcagccacc tccttgctca
    40021 gcacgtcggg cgtgttggtg accctgacgt cgcgctgccg acaagccccc acgtcgattc
    40081 catcatagcc gacaccgaag caggcgacga tctcgagcct gggcagccgg tcgatcaggg
    40141 cggcgctgac cccccgctcg ccagtgctga caataccccg gatgtcgccg ctctccgcct
    40201 cgatcagggc ttcggggtcc ggcgagctgg aaaggtggac gaggcgaaat tcctgctcga
    40261 gctgggccat ggcgttcggc cggatcggcc agacgacgag aaccgatggc tgctcgtcag
    40321 tggtggttgc ggaggtcata cggcgttccg ggcgttgtct cgttggcgct cggaactgaa
    40381 gtagcctcag cgcggcctgc tcacaagccg agccgcgagt tcaggcggcc ggtttgggcg
    40441 cctgcgcgag tgccgtcgcg atcgtccggc tgagccgggc aaccgcctcg ctcatcgcgg
    40501 ccgtccgctt ctcgaaggtc gccggaccct cgaccggcac ggtgatgcgg atcggagcgg
    40561 ttttcagcac gtcgagcgat tcgcgccgga acagcgtcca gcgcccttcg aacactgtga
    40621 gctcgggcac cgcctcggca tccggaacgg cctcacccat ctcgatatcg aaacgctcga
    40681 cagcgatctc gaccgtatag tccagcggca gcagccgctg ctgcggcagg atgaagacga
    40741 ggtcggtgcc gagcagcagc gccaggttgc gggcgacgac gcggcgcatc tgcagatcga
    40801 gcggctcgat ccagttggaa ttctcggcga ccgtcagctc gttcgggctg gtgcgcgtga
    40861 cgatgccgtt ccgatcgagg tagtcgggca gggcgatggg cgccacgccg atcaccttgt
    40921 cggtctcgcc gacgggcgat tcctggctga ctggtgtcag cgcgtagtag cggatgggtt
    40981 gcaccgccgt gcatgcgacg acgagcagca cgagagccaa ggtcagggat cggagccact
    41041 tcgctgtccc catgatggtc actcccttct gccgcgaatg atggcgctcg gatcgcgatc
    41101 gagcatgtcg gcaaagctgc ggatcgagcg ggctgccgtg gtgagctcct gcaagacccg
    41161 ctcgagctgg tagcggagag tcgagttctg accgagcatc gcatcgaccg actgcatggt
    41221 ctgctgcgcc tgggcgacgg ccgcctcggc ggatcgcgcc atcggcccgg tcgaggcctc
    41281 cagctcggcg atgagcgaac gaagatcact gccggctgca gcgagattct cgatgccggc
    41341 agccacgtcc ggatcggcca cgatgttctc gatggtcgcc gcggagcgtt gcaggctctg
    41401 cacgatgtcc tcgatcggca atgacgacag ccgatcgagc aggccgctga tcgaagcggt
    41461 gagcgcctcc agatcggtcg gcaccgaagg gatcagcggc acgccgtcga tcatctccac
    41521 ctcggccggt ggcgcgtccg gcatgaggtt catggctatg aagagatcgc cggtgagcag
    41581 gttgccggtc ttgagctggg cacgcagccc gagagagacg aggtgcttcg tccgctcgta
    41641 ctgctcctcg atggtatccg cggggtcgct gttggcgaaa cgctgcggct cgagatcgac
    41701 gtaaacccgg atcggtgcct tgccgctcgt gaagtcggga atcagctcga tctgcgtgac
    41761 cgtgccggtc cgaatacccc ggaactcgac gggggcaccg acctgcaggc cgcggaccga
    41821 gccgtcgaaa tcgatgatgt agggaatgtg cgcggcgatc cgcgaatcac cgatgctcga
    41881 ttcgtccgga aagagggtga atgtcgtgcc ggccgccgcg ggcccggccc gaccgtccgg
    41941 cgaggaaaat tcgactccgc ccgcgatcag cgaggccacg ctggcgatct tcaggtcgat
    42001 gccgtctgcc ccggcccgaa agctgatgcc gctgacatcc cagaaatggc tgtcgggcac
    42061 caccagcttg tcgtgcgggc tgcggacgaa gatcggtatt tcgaagcgct tgccgtcgtc
    42121 cgacagcttg tggccgagga tctggcccac ctcgaagcca cggtagtaga caggcgcccc
    42181 gcgatcgaca gagcccagat tctcagcgac cagcttgaac tgggtgccct cgacatcgga
    42241 gcggatcaag ggcggctcct cgaggccgac gaagctcgtg gcgctcgcac cctcggccgg
    42301 ctccacctcg atataggcgc ccgacagcag cgtgccgagg cccgtcacgc cactgaagcc
    42361 gacacgtggg cgcaccaccc agaagcgg
//
LOCUS       DAOTFI010000005        40605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1038_length_40605_cov_7.359581, whole genome shotgun sequence.
ACCESSION   DAOTFI010000005 DAOTFI010000000
VERSION     DAOTFI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 40605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40605
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1038_length_40605_cov_7.359581"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="P5558_00855"
     CDS             complement(<1..243)
                     /locus_tag="P5558_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRY22888.1"
                     /translation="MQAAIVTIAQQKGGAGKTTLAIQLATAMRQAGRSVAVVDIDPQG
                     SLTAWVRLREHEARDAAELRFCWAMVTMAACMGGAPP"
     gene            complement(267..1259)
                     /locus_tag="P5558_00860"
     CDS             complement(267..1259)
                     /locus_tag="P5558_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HRY22889.1"
                     /translation="MSSGGRRCTRDQPVDYRFEGETVALLFYSQDDDPHAWGAALRAL
                     LPELDLRIWPEIGDRREIEAALVWLPPPGLLASLPGLRAIFSLGAGVDAMLGQEDLPD
                     VPLCRLVDPSLTRTMAEFVLAQVLYYHRDLDLYAAQQQAARWRLRLPQAAASRTVGVM
                     GLGELGGAAARLLAGHGFEVLGWSRTKRTIDGVRTFAGSAGLAPFLARSEMLVCLLPL
                     TAETEGLLDAALFAGLPAGAVLVHVGRGRQLVPADLMAALDTGQLRGAAIDVTPEEPL
                     PADDPLWRHPGVRLTPHVASYAQPATAAATVVENLLRLRRGEPLLAVVDRSRGY"
     gene            1379..1849
                     /locus_tag="P5558_00865"
     CDS             1379..1849
                     /locus_tag="P5558_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRY22890.1"
                     /translation="MPGRRNYRLPLSALVAGLVPARFVLAGLALAGLALAGIPIAGPA
                     DAASLQVTDQLQRAVFDAADLNGDGAIDEAELAADAIAAFVGLNATGDNGRTIDELVG
                     VTPEAFAAVDANADGRIDLDELRLAKLAAFNAADRDQDGALTIDELLAEPIGAE"
     gene            1849..2448
                     /locus_tag="P5558_00870"
     CDS             1849..2448
                     /locus_tag="P5558_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRY22891.1"
                     /translation="MKTMTRAVRPLAASLTVALLAACTSSANQPYYLPMTIEPGAGPE
                     GTAGNEALILNSSVLVGDLLVGGIAAERLNREGDIEPASGPVVITDDAALDRDARRQV
                     AFDRLAVERRVQLADDLDQQLYFESWMSTRVEMPAMSRDEVRRAQELLAQQGYDPGPI
                     DGLYGPRTKAAVERFEQARGLPVTGTVSTGLIDRLRLEA"
     gene            2534..2989
                     /locus_tag="P5558_00875"
     CDS             2534..2989
                     /locus_tag="P5558_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22892.1"
                     /translation="MSMHRNGLVAAALTMLLAGCQEGPPPPGSAASAAPEQSVERRVE
                     AALRSNGLTVEQTPNGLRATSSSDRFARCDAVEVGGGDSSSARQFTPPESVSATAIVT
                     FTPSGSGTEATWQTSYSGNYLDRESNEPFERPCEGTGALEDLLQKAVDG"
     gene            3058..3495
                     /locus_tag="P5558_00880"
     CDS             3058..3495
                     /locus_tag="P5558_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22893.1"
                     /translation="MTCGVALAYLLAGCQAAPPPLSASSPAPVDTVRSNVAAALEREG
                     LVVEKTAGGLRATSDAERFTKCVPVLVGGSSGSDSSRKMIRVDERSATAIVRFTSRDG
                     GTRADWHTSYSGRYYDIIRNDHFTKGCEGTGTLEALLTAAVAG"
     gene            3582..4076
                     /locus_tag="P5558_00885"
     CDS             3582..4076
                     /locus_tag="P5558_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22894.1"
                     /translation="MTHRRHGPYIRPLLPMLALGLLLPACAPAPRPGPPPPARVDSSL
                     SPEAATAALTEALGRNGLAIDRAPGGLTATSTDERFMLCDTIWVRERSASDKSPRGRF
                     TSPDSTRIRADVRIGPGPLGSTVTWQTLASGNYLNRFDNIRFDARCATSGELEQLIED
                     AVRG"
     gene            4147..5340
                     /locus_tag="P5558_00890"
     CDS             4147..5340
                     /locus_tag="P5558_00890"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRY22895.1"
                     /translation="MTFKTLDALDARDKRVLVRVDFNVPMQDGEVTDATRIERAARTL
                     TELADKGGRVVALSHFGRPKGRHEPSMSLAPIAEALADVLGREVAFATDCIGEPAAQV
                     VDGLEPGGIALLENLRFHSGEEKNDPAFAEALARLGDAYVNDAFSCSHRAHASVTGLP
                     ERLPSYAGRLMQAELEALTTALGGGDRPAAALIGGAKISSKLMVLGELLGKVDLLIIG
                     GAMANTFLAARGVGIGKSLSEPDLLDNARRIMAEAEAAGVAIVLPIDGVVAETFEPHA
                     DCRTVPIAGVGEADMILDIGPGSITDIKDRLASVKVLLWNGPMGAFEVPPFDVGTTEV
                     AKAAAVLTQAGKLKTVAGGGDTVAALAHAGVVDRFTYVSTAGGAFLEWLEGKTLPGVA
                     ALETG"
     gene            5351..5776
                     /locus_tag="P5558_00895"
     CDS             5351..5776
                     /locus_tag="P5558_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22896.1"
                     /translation="MRSEAPPLAVLVHHRLEAAAAIEHAKARGEAIELCIPAGLAGPA
                     FVKALEATLQHPLTALCDDEPGLVMAALRAGLRRLVFTGPENVRQRLAGMAAGLGAEV
                     LASPPGPLVVPGRGGSWMTPAVCVSSAPTDPPLAKGTAP"
     gene            5773..6684
                     /locus_tag="P5558_00900"
     CDS             5773..6684
                     /locus_tag="P5558_00900"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRY22897.1"
                     /translation="MSDRVKTILSWYEGENPGVKGNLYRLLMQGRLGGTGKLVILPVD
                     QGYEHGPARSFAKNPAAYDPHYLYQLAIDAGLSGYAAPLGPLEQGADTFAGQVPTILK
                     LNSANSLHSGIGDQAVTGSVKDALRLGCVGVGFTIYPGSEANYGMMEEFRELSAEAKA
                     NGLVSVLWSYPRGGNVTKEGETALDIVAYAAHMAALLGATIIKVKPPKAGVDLKAAQS
                     SYENVPMDSLKDRIAHVMQCCFNGKRLVVFSGGEAKSTDGLLGEIREIRDGGGSGSIV
                     GRNSFQRPRAEALDLLGKIIEIYKQGA"
     gene            6681..7310
                     /locus_tag="P5558_00905"
     CDS             6681..7310
                     /locus_tag="P5558_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRY22898.1"
                     /translation="MSRSRRRTGRKDPDTEPGNWVDVADQGCRLLVACPAGLDPAPFL
                     AIAAVAGLVLPGEPGADTIDVVRAVGRAAFLVDRPDLVRALGADGVLLNRPGAVGPAR
                     LLLDQGELIGAAVGGTRHDAMVAGEDGADYVLFGTPGKTPADGIENLAAHVAWWTGIA
                     VLPCVVAGRFTADDVRRLAAAGADFLLPDVDDAAGLAALAAVLPVPDRS"
     gene            7322..7888
                     /gene="efp"
                     /locus_tag="P5558_00910"
     CDS             7322..7888
                     /gene="efp"
                     /locus_tag="P5558_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRY22899.1"
                     /translation="MKISVNDVRPGQVLEHQAKLWRVVKTEHVKPGKGPAYLQVELKG
                     VDHPTKLNERLRTADTIERVRLDETDYQFLYDDGDQLHFMNQESFEQVALPKELVGDG
                     VVFLKEGMIVTVQSYEGRPLSLALPETVVCEIVQADPVVKGQTAAASYKPAILDNGVR
                     VMVPPHIEMGTRVVVNTADSSYVERAKD"
     gene            7892..8689
                     /locus_tag="P5558_00915"
     CDS             7892..8689
                     /locus_tag="P5558_00915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRY22900.1"
                     /translation="MARRSANCEMMVRAAIKAARGLVRDFGEVEQLQVSLKGPADFVS
                     NADRRAERILKEELRRARPEFGFLMEESGEEVGSQPQNRWLVDPLDGTTNFLHGLPHF
                     AVSVALEQFGELTTAVVYDPIRDELFWADKGGGAYVNDRRLRVSARKELGQALVGCGL
                     PVQTWPGREKGFTTQMNAVADHVAGLRRLGTASLDLAWVAAGRQDGFWEYGLKPWDIA
                     AGILLVREAGGRVGRLEGDDDLLAEGTLVAATGGLFPKLTELLQAAG"
     gene            complement(8674..9888)
                     /locus_tag="P5558_00920"
     CDS             complement(8674..9888)
                     /locus_tag="P5558_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY22901.1"
                     /translation="MRGFLELLRQQGPLLGFGALFCFASSFGQTFFIAVFGGAIRSEF
                     GLGHGAFGATYSAATLASAVTLIWLGRLVDRVPVARLTSATVLGLALACLLLSFAQGP
                     LTLALAFYGLRLAGQGMAPHIAISTMAKRFSASRGRAISIVTLGFPLSEILMPHLAVA
                     AIGLGWRWGWRGFAVLLVVLALPALLGLIARAERPRASTAVAPAISLVPSRRQGEVLR
                     DPRFWLLLPGVLAPSIIVTGIFFHQVHIVGAKGWPLGLFAATFTIYALGSVASSLASG
                     WAVDRFGASRSLLAFPLLLATGCLLLASSDAFAAGILMMIFLGLASGGQSTAVNALWA
                     ELYGTEHLGAIRSLAMAFSVFGSALSPIVFGAVFDRGVPVEGLAAAAACYVVLAGSLF
                     VPAFRREAQPAA"
     gene            10031..11440
                     /locus_tag="P5558_00925"
     CDS             10031..11440
                     /locus_tag="P5558_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY22902.1"
                     /translation="MTVIEALVAALGEGVVAFDAESRDRQSRDLSTVPAARPLAVLRP
                     RTTAEVATALGICHAHGQGVVPQGGRTGVSGGAVPRPADIVLCLDRLSGIEAIDTAAA
                     TMTVLAGTPLETVQGAARDAGFLCPLDLGARGTCAIGGNIATNAGGNRVVRYGMTRQM
                     VLGLEVVLADGTVLDMLNTMIKNNAGPDLKQLFIGSEGSLGVITRAVLKLEPLPVSSA
                     TAFCGLAGLDAAIAFLGRARSGLSGLLSSFEIMWPGYYDLIIGTPAGSGATGVRPPLE
                     GRHGMYILIEAQGGEPAADADRFERFLEACLEDGLIEDAAIAQSGEAARAFWGVRDAV
                     IEFNALLGQRVEFDLGLPIGRIGTCTRAIEASLRQSWPDARIIWYGHLGDGNIHLQVA
                     VPSVRPHPDHAIDEIVYDHLREHGGTITAEHGLGTLKAPWLAHCRTPAEITLMHGLKR
                     TLDPMGILNPGKVLVDGAT"
     gene            complement(11751..13064)
                     /locus_tag="P5558_00930"
     CDS             complement(11751..13064)
                     /locus_tag="P5558_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22903.1"
                     /translation="MGAEKAFQALATQLHGADDRTLLKLVAVVDQLAKRGSLDQLLDE
                     HRARLAVIRPPRPMTLGRLVVLPLEELLIDGAEWTPGMTRVPRDRLGRLIELVLEAID
                     PDLRRGAASQLAGKSMHDSALALDVGREIWPAAGLAADAILARGRQTRDTELRELLTP
                     LRIMQNLMPVAESLVTTIWSLPDRPMLALEPAGRARIGALLALASAQGRDCFLLTAEL
                     LVARTELPLAIIEAVLDGELELADRERQQASAMIAEACQNEMVRLFRSVSLLPAGTGP
                     SALVGPLRTLVANLESLQEVAQKVKFDHRELRRLKAEVFTLIEARLEASLGGALLHAF
                     GALDDDTKSADCRALEAHAMAAANMRLMAKRIGLATKIDFVFAKALERFRAILEEAAG
                     PGAGPMGATMDRIRIIELLFDSRSAMQSLTRLRQLAHAARAREPA"
     gene            complement(13131..14237)
                     /locus_tag="P5558_00935"
     CDS             complement(13131..14237)
                     /locus_tag="P5558_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22904.1"
                     /translation="MARDDRQHRHLDLAERSVGLPFTTPLISGAKARLVATGGVELLA
                     PNPSGQLGYYVMDLRSLRDYCQLTVHDELLLEKLLGVDVVTPATVRRAAREVAMEGAA
                     GRDAAAAAKRAAERDAAIVNLTNYLMLMRLLRAVGIDERELQRSGGTGRAVQLEIKRR
                     LTSLSPRLGMTADEVIDTVGDIAFYAAAIGFADGELETRHRRVLERLSPFAADMRHWA
                     ALETGESKECAEQIADCAGWTSNKVAPLIADCQVRLEDIPELVRAWRHDRGALKGRFA
                     LPDWLLDGWPEILALWDSASGDGRAAQRAVAAQIQRLMPLAQTQAEGRSVEVMRVKGT
                     MVHSRLVKLHEDWRTGVVMGGIRARTEALRAGVA"
     gene            complement(14344..15648)
                     /locus_tag="P5558_00940"
     CDS             complement(14344..15648)
                     /locus_tag="P5558_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY22905.1"
                     /translation="MSPGEVAAILFGLFGVLVVLRVPVAFALGLAVVPVFFFDERLTP
                     ILLLREMFKSYNAFVLLAVPFFLLAANLMNGAGITDRLIAFARSLVGHLPGGLGHVNV
                     VVSMLFAGISGSSTADAAGIGSLLIPQMKKQGYDASFAVAITACSSVMGVIIPPSILM
                     VVWGGLMSVSIGGLFLAGVVPGVLIAVAQMATVYVYAKRRGYPIYARSTLKELAAAFV
                     GSLLALMTPAIIVGGIVGGLFTPTEASVVAVIYSLILGLFIYRSVSFQQLPTILYDSA
                     RFAALSLFCIGTASAFGWALAYFKIPAALVAQIETLGLGVTGTGMLVALAFLLIGMFI
                     DAIPAIIILGTVLYPVVEHVGMHPIHFAIIGVVSLAFGLVTPPYGLCLLIAAAIGEIK
                     LIRALRDVAIILLPMIGILMLIIVVPDIILALPRWLMPRFVQ"
     gene            complement(15645..16145)
                     /locus_tag="P5558_00945"
     CDS             complement(15645..16145)
                     /locus_tag="P5558_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY22906.1"
                     /translation="MRRFVDGYYRVLQLLLTLLIAVLIVPVTMQVLSRYTGLIPRYIW
                     TEEIARFAFIWMIMVGAGIAVRDGTHFDVDLLPEPKTKAGRVAARLLVNLTIGAVGVI
                     FLIWGWDFAILGSRQSSEIAGLPMLSIYIAWPWAGATWLLFVGERILVDLGMVDPPRP
                     RTVGAG"
     gene            complement(16209..17195)
                     /locus_tag="P5558_00950"
     CDS             complement(16209..17195)
                     /locus_tag="P5558_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY22907.1"
                     /translation="MNRITRMLAGGVALLALAGTASAQGVTLVGASQFDENHAFTKTM
                     REFERLVGECYSGDVTFDLHLNSELGLEKDYFENMSQGIAVDYAVFSPAHASTFSQMA
                     PLMDMPFLFRDLDHWNKVMDAGALDPIADDILEKANVLIIGYAGGGTRNVFANRPVRN
                     MEELKDLPIRMQGAPIWTRVFDALGAAPTVIAYNEVYNAIQTGVIAAAENEAAGIEQM
                     KFYEVGPDLALTQHAITVRPLGFSNATFERLDPELQACIVESGKAAGKLGREIESGQD
                     AEKLKAMEEKGWLTPYEFTDRAKLLELAAPVMQAYAEELGASDVYANIVSVQ"
     gene            complement(17281..18663)
                     /locus_tag="P5558_00955"
     CDS             complement(17281..18663)
                     /locus_tag="P5558_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRY22908.1"
                     /translation="MCLAILILLALHAFVPPPPALAQTDEAAAEAAPAEAVAEDEGPE
                     LPEELLDPAVDPQVLEYRLVPLTKGELAALAGEWLQIVKAKTEEVARLQVEISQSEGN
                     VAEAKRDELTELVAERKKLFNNYDKVVAAWEKKGGDEAAVADYRAYKSSIIIEETRTA
                     DFRTLMNQALAWLVARDGGIELGLDIAIVVGAFLVLLIVARIVRRVVGRYIGRIPNLS
                     KLLQAFLIAVIYWLVLAIGIMVVLSALGVDVSPVFALIGGASFIMAFAFQDTLGNLAS
                     GLMIMINKPFDQGDYVDVGGVAGTVKSVSIVATTVTTPDNQLIVIPNKNVWGNVITNV
                     TASATRRVDLVFGISYDDSIPDAIRVMHEAVRQHPLVLAEPAPVIRVNELADSSVNFI
                     CRPWTKTGDYWTVYWDLTQRIKESFDAAGISIPYPQQDVHVKASAAPGYPQLLPEPSA
                     TQPSEGRSLP"
     gene            complement(18996..20936)
                     /gene="dxs"
                     /locus_tag="P5558_00960"
     CDS             complement(18996..20936)
                     /gene="dxs"
                     /locus_tag="P5558_00960"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRY22909.1"
                     /translation="MTQTPLLDQINEPRDLRRLQRSSLGQLASELRQETIDAVSVTGG
                     HLGAGLGVVELTVALHYVFDTPYDRLIWDVGHQCYPHKILTGRRDRIRTLRQPGGISG
                     FTRRSESVYDPFGAAHSSTSISAGLGMAVADQLEGRDRNVVAVIGDGAMSAGMAYEAM
                     NNTGALRQRLIIILNDNDMSIAPPVGAMSAYLSRMVSSHTYRSFRHVAKQLASHFPRP
                     LQRAAQRAEEYARGMATGGTLFEELGFFYIGPLDGHNLDHLLPVLENARDDPDRGPVL
                     IHVVTQKGKGYPPAEAAADKYHGVARFDVATGAQVKSRSNAPSYTGVFAQALTREAEA
                     DPRIVAVTAAMPSGTGLDRLRQRFPGRVYDVGIAEQHAVTFAAGLACEGLKPFCAIYS
                     TFLQRAYDQVVHDVAIQNLPVRFAIDRAGLVGADGSTHAGAFDITYLATLPNMVVMAA
                     ADEVELCHMVATAAAHDSGPIAFRYPRGEGVGLELPERGSVLEIGKGRVVRRGNRVAL
                     LSLGTRLQDCLKAADELLAMGISTTVADARFAKPLDAALLVELARGHALLITVEEGSI
                     GGFAAHVQHFLLEHGLLDGAHARLRTMMLPDRFLEQGSPTGQLAEAGLDAPAIVAKVR
                     ETLEAFETATSTNRAPAVPDRA"
     gene            complement(20963..21856)
                     /locus_tag="P5558_00965"
     CDS             complement(20963..21856)
                     /locus_tag="P5558_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY22910.1"
                     /translation="MDGPAVERALRDHAAAVETWLDEALPRPEGATAALVRAMRYAAL
                     GGGKRLRAFLVKATGDLFEVPAEQSLAVGGAIELLHAYSLVHDDLPAMDDAAMRRGRP
                     SCHKAFDEATAILAGDALQALAFELLARGVTGIDDATSRALVLGLSRAGGPAGMCGGQ
                     MIDLEAERLALDLTGIRELQALKTGALIRFSCEAGCLLGRAGDVDRQALLAYGGRLGL
                     AFQISDDLLDLRGDAALVGKDVGHDAGQGKATFITALGPAGAEAELRRLEAEAAGLLA
                     GFAGRANVLEALFGFVIHRTH"
     gene            complement(21873..22145)
                     /locus_tag="P5558_00970"
     CDS             complement(21873..22145)
                     /locus_tag="P5558_00970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRY22911.1"
                     /translation="MAKQEPQEATAAAEPAIGSMSFEVALAELEAIVQRLERGQLDLE
                     ASIAAYERGTALRQHCATKLREAQLRVEKITFDRNGKVGLEAQDDT"
     gene            complement(22226..23161)
                     /locus_tag="P5558_00975"
     CDS             complement(22226..23161)
                     /locus_tag="P5558_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRY22912.1"
                     /translation="MNVLYYTHPSALRHDAGPGHPENAGRLRAIEALLAAERPAFLVR
                     REAPQATLAQIERAHPAPYPERILAAEPMDGYVRLDPDTLMSSGSAEAALRAAGAGCA
                     AVDAVLGVEARRAFIAMRPPGHHAEPQQAMGFCLFDTVAVAALQARAVHGIERIAIYD
                     FDVHHGNGTQAIFWDDPLTLYLSTHQMPLYPGTGAAAERGSHDNVVNVPCAAGTGSAL
                     WREKVEGTILQRLDAFRPELLMISAGFDAHEADPLAQMRLTEADFAWVTGKLVEVAER
                     HSQGRIVSVLEGGYDPDALARSVLAHLEGLAGTTA"
     gene            complement(23185..23481)
                     /locus_tag="P5558_00980"
     CDS             complement(23185..23481)
                     /locus_tag="P5558_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRY22913.1"
                     /translation="MNRVRTHPGEVLREEFLVPLGLSANALALALRVPATRITAIIRE
                     REPRSITADTALRLARYFGTTPQFWLNLQTAHDLSLVQCEKGEAIQRDVQPRAA"
     gene            complement(23491..23766)
                     /locus_tag="P5558_00985"
     CDS             complement(23491..23766)
                     /locus_tag="P5558_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY22914.1"
                     /translation="MIRSFADKRSAAAFRGEFAPVWRSMSAVLACKLDMLDAAMELDD
                     LRQPPGNRREKLKGDRDGQWSIRVNGQWRICFVWNDGPEAVEVVDYH"
     gene            complement(23836..27336)
                     /gene="mfd"
                     /locus_tag="P5558_00990"
     CDS             complement(23836..27336)
                     /gene="mfd"
                     /locus_tag="P5558_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRY22915.1"
                     /translation="MTTATLLDPVDTAGAAGERILLGNALGGLEARFVAERLDSGATD
                     TLLYVTRDAQRSTALAALVRFFAPGIEILTLPAWDCLPYDRVSPNATVVAERLATLHR
                     LARERGRRKLLVLVSANALTQLLPPPDAVRASHLQLRASARVERDGLLAHLERNGYQR
                     VGTVVDPGDYAVRGGLIDLFPTGAEQPVRLDFFGSTLESIRRFDPATQRTTDRLKTVE
                     LGPVSEVLLDDAAAARFRAGYLRQFGTVTEDPLLESVAAGRHFPGMEHWLPLFHEKLV
                     TVFDYLDGGFEVAFDHLAEDAVAARQAAIGEHFEARTAPAAKSGAFGSIPYRALPPHM
                     LYLDEESLRRRLAGRPAWLFSIFGPPPVPPSGVAGLRDLGGRPGRDFARERSDRAINL
                     FDAIVAHLRELKAAGESPIITVHGVGAADRLQQVLVDHGLEPPLRIEALAELVPGATH
                     LAVLPLETGVSAPGLCLLGEQDLLGERLTRRQARRAKAADKFIQDVGTLAENDLVVHA
                     EHGIGRFEGLVTLEIGGAAHDCLKLLYAGGDKLFVPVENLDILTRYGNAEQEVQLDKL
                     GGVGWQNRKARIKQRIKELADELIRIAAARALRKGEPLTLAPGLYEEFAARFPFDETE
                     DQLRAIQTVLEDLASGQPMDRLLCGDVGFGKTEVALRAAFVAAMSGKQVAILAPTTLL
                     ARQHFKNFGERFKGLPLRVEQISRFVTARQANEVKKGLADGTVDIVIGTHALLAKSVS
                     FRNLGLVVVDEEQHFGVSHKERLKQLRAEVHVLTMTATPIPRTLHMALGGMKDLSIIA
                     TPPVDRLAVRSFVMPVDPVVLREAILREYHRGGQVFYVCPRIKDQPKLKEELERLVPE
                     VRVAIANGQLPAKDLEAVMSAFYDRQLDVLLATNIVESGLDIPTANTLIVHRADLFGL
                     SQLYQLKGRVGRSKVRGYAYFTVPANKILQETAEKRLHVIQSLEGLGAGFQLASHDLD
                     IRGAGNLLGDEQSGQIREVGFELYNHMLQEAVAALRATGDAEATTEWTPHITIDAAAL
                     MPESYIADLDLRLAMYRRLASLEEPEDIEGFAAELIDRFGSLPAETEHLLQIVAIKQL
                     CRKANVQKLDVGPKGVVLAFRENRFAEPERLIQHIAGSKGRMRVRPDHKLVVMQDTTT
                     PADRLKAAKKVVDELARLAA"
     gene            complement(27333..27596)
                     /locus_tag="P5558_00995"
     CDS             complement(27333..27596)
                     /locus_tag="P5558_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HRY22916.1"
                     /translation="MSDARTTRLKRLAHRSRYRGFKEVDLHFGQFADRHLAGLDGTEL
                     DQYEALLDEPDQEVWAWLSGARHVPARHDHGVFRLLQSFEPVS"
     gene            27707..29842
                     /gene="recG"
                     /locus_tag="P5558_01000"
     CDS             27707..29842
                     /gene="recG"
                     /locus_tag="P5558_01000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRY22917.1"
                     /translation="MAEPAKGTGGEMARPAELYALFRPLESLPGVGPKVARLCARLLG
                     RSEPRLLDLLFTPPVRRRDIADRRRLEPEDEGRLVALELVTEEHRPGHGRSPYRIRCT
                     AERQNVTLVFFHARQPWLERRFPPDRTVRVIGRPELFNDQWQIVHPEPASEGGRDATV
                     YRLIEGLGALRLKRLIDDALAEVGELPEWHDPATLEAHDLPPWRAALAVLHGRVPGEV
                     PLDDAAIERARRRLALDELTATQLALALVRRSRDRVPAPPLAGPSPRTEAMLASLPFT
                     PTGAQARAIGEIATDLARPIAMARLLMGDVGSGKTLVALAAMLRATDHGRQAVMMAPT
                     EVLARQHARSLGALAEPLGLEIDLLIGGDSAADRRRALERIASGEAAITVGTHALFQE
                     GVTFNNLALAVIDEQHRFGVAQRLGLVAKSHATHLLLMTATPIPRSLVLSAYGDVETS
                     RLDEKPPGRQPIVTAALPRRRLEEVLERVAAAIGRDERIYWICPSVAEATTDGPADDD
                     TGRIAAETRFGELQGRFGKTVGLVHGKLKTAEKTAAMQAFASGRTRLLVATTVIEVGV
                     DVPEATVIVIDGAQHFGLAQLHQLRGRVGRGSRPSACVLLYDPPLGEAARARLAILRE
                     SEDGFRLAEEDLRLRGPGEVLGVRQSGLPELRFADLADDGDLVATAHDGVAVALAQNQ
                     ALAGERGRALRLLLHLFERYEAVGCLAAG"
     gene            29983..30444
                     /gene="aroQ"
                     /locus_tag="P5558_01005"
     CDS             29983..30444
                     /gene="aroQ"
                     /locus_tag="P5558_01005"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRY22918.1"
                     /translation="MPPAVLVLNGPNLNLLGRREPAVYGHETLADLEAACVAAGSDLG
                     LAVTCRQSNHEGELIDWIHAARETTAAILINAGAYSHTSQAIPDALRAYPGIIVELHL
                     SNIYAREAFRHHSHISATATAVICGLGSQGYHLALRAVAERLAASAQHPDG"
     gene            30449..30901
                     /gene="accB"
                     /locus_tag="P5558_01010"
     CDS             30449..30901
                     /gene="accB"
                     /locus_tag="P5558_01010"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRY22919.1"
                     /translation="MGKLNIDTDYVAALAELLQRTGLTEIEISEGDARVRVVKKPAPV
                     YEAHVAPRAAAPVLADSEPTTAPANEPAHPGTVTSPMVGTVYLYPEPGADPFVAPGQT
                     VREGETLLIIEAMKVMNQIRAPRSGRIARIFVDNATPVEFGEPLLIIE"
     gene            30928..32271
                     /gene="accC"
                     /locus_tag="P5558_01015"
     CDS             30928..32271
                     /gene="accC"
                     /locus_tag="P5558_01015"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRY22920.1"
                     /translation="MKKVLIANRGEIALRILRTCHEMGIGTVAVHSTADANAMHVRLA
                     DESVCIGPPGAGESYLNAAAILSAAEITGAEGIHPGYGFLAENADFAQMVVEHGFTFI
                     GPDPGHIRMMGDKVVAKASVAAAGIPTVPGTDGAVTEDAMAFTAADTIGYPVLIKAVA
                     GGGGRGMHVVRSRAELGAALRNARNEARAGFGDERVYLEKYLDHPRHIEIQVMADRHG
                     NVVHLGERDCSLQRRHQKLIEESPSPVITDSERRTIGETVVEALGKLGYLGAGTIEFL
                     YQDGRFYFIEMNTRIQVEHPVTEMVTGIDLVREQLTVAMGHPLSFTQKQVRLEGHAIE
                     CRINAEDPQTLLPQPGRIVAYHPPGGPGVRVDSAIYSGYTVPPFYDSLIAKLIVHDKD
                     RERCIARLERSLAEFVIDGIPSSIPLLRRVLAEPEFRANRYDTSWLERFLARPSA"
     gene            32317..32970
                     /gene="aat"
                     /locus_tag="P5558_01020"
     CDS             32317..32970
                     /gene="aat"
                     /locus_tag="P5558_01020"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRY22921.1"
                     /translation="MQLLTPELVLSAYSIGLFPMANDRDDPTIHWIDPLHRGVLPLDR
                     FHVPRSLRKTIRRGRFELSVNRDFPAVIAACAEATPDRPRTWLNDDIIRLYVDLHRRG
                     HAHSIETWEAGRLVGGLYGLALGGAFCGESMFSRATDASKVALVELVARLRIGGFVLL
                     DTQFVNDHLAQFGIEEITRSAYKARLKKALTIDASLPVNPGSFAAVLVGRASAADDS"
     gene            complement(32975..34561)
                     /locus_tag="P5558_01025"
     CDS             complement(32975..34561)
                     /locus_tag="P5558_01025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY22922.1"
                     /translation="MSISLGFHGAASTVTGSCFLLETPKGRLLVDCGLFQGTKTVREL
                     NYNDFPFEPASIDALLLTHAHIDHSGLIPKLVRKGFAGSIVATGPTADLLEFMLPDSG
                     YIQESEVERLNRRNRQRGRPQVEPIYTQADAIRTLEQVRTCDLGEWLEPLPGVRARYW
                     NAGHILGSASIEIEVDAAGEPPLRLLFSGDIGPDEKTFHATPEGPQDVDYLIVESTYG
                     NRDRDEVTLEQRRARLCREVEEALAAGGNLLIPAFAVERTQEVLQVLSALIRAGEIPQ
                     VPIFIDSPLAIKVTQIFAQHQADLEDVGGKGDFFDAPWFRFTESVEESKAINKVKGGA
                     IIVAASGMCDAGRIRHHLKEHLWRRDSTVLFVGYQAPGSLGRIIQSGERRVRIHGEEI
                     KVNARIRSIESFSAHADQGELVAWVRDRLPVRKRIFLTHGEPDAMVAFRLKLAEIGCD
                     PEHVIAPALDDRFDLEAEESAIRHGDARRLSEEAAMASSDWHNAYARLVLDLSGRLKE
                     LPDDAARTALLERLRRSFDG"
     gene            complement(34558..35493)
                     /locus_tag="P5558_01030"
     CDS             complement(34558..35493)
                     /locus_tag="P5558_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRY22923.1"
                     /translation="MRDIKDDHLNRPSAREDLEHSQDVVPSPQSQAASYRLAFADTDF
                     LLREELRPVRLQLELLKPELELQAMLVESTVVIYGSARIGDPEEARAALASAEAAAGD
                     DPKDPARQHELERCRRAVARSRYYDEARRFGRLVTEHAQDGPVRQFVIVTGGGPGIME
                     AANRGAAEAGGVSIGLNITLPHEQSPNRWVTPELSFQFHYFALRKMHFLMRARALVVF
                     PGGFGTLDELFEALCLMQTQKVRKIPVLLFDREYWERIVDFDALLEEEVVSASDLELF
                     RFVNTAEDAWRHIREHYGLIAESSDGASQLSGSIA"
     gene            complement(35635..36015)
                     /gene="phaP"
                     /locus_tag="P5558_01035"
     CDS             complement(35635..36015)
                     /gene="phaP"
                     /locus_tag="P5558_01035"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HRY22924.1"
                     /translation="MSTKSALNATPMMPKFDVDAVMALHKANLDTMVAAQKIMFDLAQ
                     TVAKRQSELLKDAFSSTESMMKGFDGKKQPQAYMDDAKVVLEKAMAEAKETMDLGMKA
                     QTEVVDLFVKRATANFDEAKTLAA"
     gene            complement(36148..36651)
                     /locus_tag="P5558_01040"
     CDS             complement(36148..36651)
                     /locus_tag="P5558_01040"
                     /inference="COORDINATES: protein motif:HMM:NF037928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HRY22925.1"
                     /translation="MALDGILRRSIRGDIMPLERKTIWLLVADAARGRLFAVDRRSRR
                     FVLLRSWADDAAAGKGLDLVADRPGRTFDSAGQGRHAMEPTTPPKTVAKARFLAMLAG
                     HLNAEAKRRAFDELHLVAAPRALGELREQLDPPVLERLAGDQAKDLTPLGQPELEAAL
                     GDRFWPS"
     gene            36789..37721
                     /locus_tag="P5558_01045"
     CDS             36789..37721
                     /locus_tag="P5558_01045"
                     /inference="COORDINATES: protein motif:HMM:NF023431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HRY22926.1"
                     /translation="MAKSAAALLATFTVGQAKAALAPLDPAAVGDWHYAPRAWPGLPL
                     RAMDETQRRLLWRLVDLTLSESGAARARAILVLEAILGELEGRPTFRDPENYHFRCFG
                     RPSAEALWGWRFEGHHLSLTAMLDPERGVLPTPIFMGANRATVPPPHPRAGLRTLPEE
                     ADQGFALLHGLDDARRARAILAERSPGDVITGPGRELSLRALEGVPLADLLDSSRAAA
                     LALVELYVGRLTPALATTEMAAIREAGTDRIHFAWAGSTTPGRGHYYRLQGPSFVIEY
                     ENSQNDANHCHSVWHNPLSQFGRDLLRGHFHHSH"
     gene            complement(37836..39710)
                     /locus_tag="P5558_01050"
     CDS             complement(37836..39710)
                     /locus_tag="P5558_01050"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HRY22927.1"
                     /translation="MSFKTTALAGVSLTALLIAPATLCAASIASVEFVGMEAPSTAAD
                     MARIYSSARAEVTYDDGSKKVFPLRYEVLYRNLDRIGSNPYEAGRLYDAFGQGLIDPY
                     GQPVISENPDGSTLTRVPGQPSAPNGDPTAYHLVQWEADWLLSDGKEASKTEGWYPRM
                     PMIMNRNLVAQERQTGRLSVIDQANVDFSGVGGLWIPCAASETPWGTHLAGEEDYDLY
                     DRGKVDASLAGLTELYFGNTRKANPYGYGYPVEVSVGRDGQSSVKKHYSMGRASWELA
                     RVMPDGKTVYYGDDGRYTGLFMYVADRAGNLGVGTLYAGRWDQQSDQNGGSANLTWIK
                     LGHAADYEVARMIEEEQFSSTESSIFAISDEPKDGFTAIKAGSKNIEHVKLNPGKERA
                     AAFLESRRYAASLGATTEFNKMEGVAVDPRDHKVYIAMSRIQEGMLANPEDPADHIKL
                     PELGAGGVYVLETASGVADSNGDPIASDWAAVTMAGVPGLMGVDLAQSDDMGNTADPD
                     HIAEPDNIAFMPRMRTLFVDEDSGRHTNNFMWAFNVDTGKLARVASGPAGAEMTGLNV
                     VEGWNGFAYVLTSFQHQGDDVGDLEIKDAALKAELETLIDPFVAVIGYLGGLPGSDVE
                     "
     gene            complement(39980..>40605)
                     /locus_tag="P5558_01055"
     CDS             complement(39980..>40605)
                     /locus_tag="P5558_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY22928.1"
                     /translation="DIQFQEGTFSVAGTDRTIDIMSLAQKARTMASPAEGIEGGLDAE
                     TTIKLDAWTFPNGCHIAEVEIDPDTGIVTIVDYNVVDDFGVVLNPMLVEGQVHGGIVQ
                     GIGQALLEHTVYDESGQLMTGSFMDYCMPRADDLPSFDFSTIEVRCANNAMGVKGCGE
                     AGSVGSPAAVINAIVDALQPLGIRNVDMPATPEKLWRQIQGAQSKAA"
BASE COUNT         6557 a        14258 c        13661 g         6129 t
ORIGIN      
        1 tggaggagca ccgcccatgc aggccgccat cgtcaccatc gcccagcaga agcgcagctc
       61 cgccgcgtcc ctggcctcgt gctcccgcag gcgcacccag gcggtcaggc tgccctgcgg
      121 gtcgatatcg accacggcga cgctccggcc cgcctggcgc atggccgtgg cgagctggat
      181 cgccagcgtc gtcttgccgg cgccgccctt ctgctgggcg atggtgacga tggcggcctg
      241 catgggcggt gctcctccaa cgtgtctcaa tagccgcggc tgcggtcgac cacggcgagc
      301 aagggctcgc cccgccgcag ccgcaggagg ttctcgacga cggtcgccgc cgcggtcgcc
      361 ggctgcgcgt aggaagcgac atgcggcgtc agccgcacgc ccgggtgccg ccagagcgga
      421 tcgtccgccg gcagcggctc ctcgggcgtg acgtcgatcg ccgccccgcg caattggccg
      481 gtgtcgagcg ccgccatcag gtcggccggc acgagctgcc tgccccggcc gacatgcacc
      541 agcaccgccc ccgccggcag cccggcgaac agcgccgcat cgagcagccc ctcggtctcg
      601 gcggtgagcg gcaggagaca gaccagcatc tcggaccggg cgaggaaagg tgcgagcccg
      661 gcgctgccgg cgaaggtccg gacaccgtcg atggtccgct tcgtgcggct ccagccgagc
      721 acctcgaagc cgtgcccggc gagcagccgg gcggcggcac cgccgagctc ccccaggccc
      781 atcaccccca cggtgcgcga cgccgccgcc tgcggcagcc gcagccgcca gcgcgccgcc
      841 tgctgctggg cggcatagag gtcgaggtcg cggtgataat agagaacctg ggccagcacg
      901 aactcggcca tcgtccgggt gagcgagggg tccaccagcc ggcagagcgg cacgtccggc
      961 agatcctcct ggccgagcat ggcgtcgacc ccggccccga gcgagaagat cgcccgcagc
     1021 ccgggcaggg aggcgaggag gccgggtggc ggcagccaga ccagcgccgc ctcgatctcg
     1081 cggcgatcgc cgatctcggg ccagatgcgc aggtcgagct ccggcagcag cgcccgcagc
     1141 gctgcgcccc aggcatgcgg atcgtcgtcc tgactgtaga agagcagcgc caccgtctca
     1201 ccctcgaagc gatagtcgac cggctggtct ctagtgcagc gtcgaccgcc acttgacaag
     1261 tcgggcgccg cggccgccgc caacccggca gaagctctcc ggctccggtc gagcacatgg
     1321 acccgtgcgt tgaaatgtgg catgcgcggt ggtcagaaga ggttcgagga gaaaacggat
     1381 gcccggccgc cgcaattacc gcctaccctt gtcggcgctt gtcgctggtc tcgttcctgc
     1441 ccgtttcgtt ctggccggcc tcgccttggc cggcctcgcc ctcgccggca tcccgatcgc
     1501 cggcccggct gatgctgcca gcctgcaggt caccgaccag ctgcaacgcg cggtgttcga
     1561 tgccgccgac ctcaatggcg acggcgccat cgacgaggcc gagctcgccg cggatgcgat
     1621 cgccgccttc gtcgggctga acgccacggg cgacaacgga cgcaccatcg acgagctggt
     1681 cggcgtgacg cccgaggcgt tcgccgccgt cgacgccaat gccgatggcc gcatcgacct
     1741 ggacgagctc cggctcgcca agctcgcggc gttcaatgcc gccgaccgcg accaggatgg
     1801 cgctttgacc atcgacgagc tgctcgccga gccgatcgga gccgaatgat gaagaccatg
     1861 acccgcgccg tgcgcccgct cgccgccagc ctcaccgttg ccctgctggc cgcctgcacc
     1921 agctcggcca accagcccta ctacctgccc atgaccatcg agccgggcgc cggacccgag
     1981 ggcacggctg gcaacgaggc cctgatcctc aacagctcgg tgctcgtcgg cgaccttctg
     2041 gtcggcggca tcgcggctga gcggctcaac cgggaaggcg atatcgagcc cgcctccggc
     2101 cccgtcgtga tcaccgacga cgcggcgctc gatcgtgacg cccgccgcca ggtggcgttc
     2161 gaccggctcg ccgtggagcg ccgcgtccag ctcgccgacg acctcgacca gcagctctac
     2221 ttcgagagct ggatgagcac ccgggtcgag atgccggcca tgagtcgcga cgaggtgcgc
     2281 cgggcgcagg agctgctggc ccagcagggc tacgacccgg gcccgatcga tggcctctac
     2341 gggccgcgca cgaaagccgc ggtcgagcgc ttcgagcagg cccgcggcct gcccgtcacc
     2401 ggcaccgtca gcaccgggct gatcgatcgc ctgcgcctcg aggcctgagc ggcgcccctg
     2461 tcgctaaggg accgaccgcc tatatcaagc tggagccgcg gcaaggtggc ggcatccatc
     2521 caggagaggc gtcatgtcca tgcacaggaa cggtctcgtc gcggcagccc tgaccatgct
     2581 cctcgccggt tgccaggagg gtccgccacc gcccgggtcg gctgcttccg cggcaccgga
     2641 gcagagcgtc gaacgccgcg tcgaggcagc gctgcgcagc aatggtctga cggtcgagca
     2701 gacgcccaac gggctgcgcg ccacctcgag ctcggaccgc ttcgcccgct gcgacgccgt
     2761 cgaggtgggt ggcggcgaca gcagcagcgc ccgccagttc acgccgccgg aatcggtcag
     2821 cgccacggcc atcgtcacct tcacgccgag cggcagcggc acggaagcca cctggcagac
     2881 cagctacagc ggcaactacc tcgaccgcga gagcaacgag cccttcgagc ggccctgcga
     2941 aggcaccggt gccctggagg acctgctcca gaaggcggtg gacgggtgag cctgaccggg
     3001 ggttcccgga acgcccgaaa gcccttggac ccactgtgcc cgcctgggcc agccagcatg
     3061 acgtgcggtg tcgccttggc ctacctgctc gccggttgcc aggccgctcc gccgccgctc
     3121 tcggcctcgt caccggcgcc ggtcgacacg gtcaggagca atgtggcggc cgcgttggag
     3181 cgcgagggtc tcgtcgtgga aaagacggcc ggcgggctcc gcgcgacctc ggatgccgag
     3241 cgcttcacca aatgtgtccc ggtcctggtc ggcggctcca gcggcagcga cagcagccgc
     3301 aagatgatcc gcgtggacga gcgcagcgcc acggcaatcg tgcgcttcac gtcgcgcgac
     3361 ggcggcaccc gggccgattg gcacaccagc tacagcggcc gctactacga tatcatccgc
     3421 aacgaccatt tcaccaaagg ctgcgaaggc accggcacgc tcgaggcgct gctcacggcg
     3481 gcggttgccg gttgacacgc cggcagcgta ccgactgtcg gtcgacactc gcggcccatc
     3541 ggcctatctc cctttgtcat ggtgcctgac cggagcaatc gatgacccac aggcgacatg
     3601 gtccctacat ccgcccgctt ttgcccatgc tggctctcgg cctgctgctg ccggcctgcg
     3661 cgccggcgcc ccgacccggt ccaccaccac ccgcgcgcgt cgattcgagc ctctcgcccg
     3721 aggcggcgac agcggctctg accgaggcgc tcggccggaa cgggctcgcc atcgatcggg
     3781 cacccggcgg cctcacggcg accagcaccg atgaacgctt catgctctgc gacaccatct
     3841 gggtgcgcga gcggagcgcc agcgacaaga gcccgcgtgg ccgcttcacc tcgccggaca
     3901 gcacgcgcat cagggccgat gtccggatcg gcccgggccc gctcggcagc actgtcacct
     3961 ggcagacctt ggcctccggc aactacctca accgcttcga caatatccgc ttcgacgcgc
     4021 gctgcgccac gagcggcgag ctcgagcagc tgatcgagga cgcggtgcgc ggctgaggcc
     4081 ggccggcatc ggagtcatgc cgggagaaga cggccacaac ggccaaagca tgggagagac
     4141 gatagcgtga ccttcaagac cctcgatgcc ctggacgcca gggacaagcg cgtgctggtg
     4201 cgcgtcgact tcaatgtgcc gatgcaggat ggcgaggtca ccgacgcgac ccggatcgag
     4261 cgcgcggcac gcacgctgac cgagctcgcc gacaagggtg gccgcgtggt ggcgctgtcg
     4321 catttcggcc ggccgaaggg ccgccacgaa ccatccatgt ccctggcgcc gatcgccgag
     4381 gccctcgcgg acgtgctcgg ccgcgaggtc gcgttcgcca ccgattgcat cggcgagccg
     4441 gcggcccagg tggtcgacgg gctcgagccc ggcggcatcg ccctgctcga gaaccttcgc
     4501 ttccacagcg gcgaggagaa gaacgacccg gccttcgccg aggccctggc ccggctcggc
     4561 gacgcttacg tcaacgacgc cttctcctgc tcgcaccgtg cccatgcgag cgtcaccggc
     4621 ctgccggagc gcctgccgag ctacgcgggc cggctcatgc aggccgagct cgaggcgctc
     4681 accacggccc tcggcggcgg cgaccggccg gccgcggccc tgatcggcgg cgccaagatc
     4741 tcgtccaagc tcatggtcct gggcgagctg ctcggcaagg tcgacctcct gatcatcggc
     4801 ggcgccatgg ccaacacctt cctcgcggcc aggggtgtcg gcatcggcaa gtcgctgagc
     4861 gagcccgatc tcctcgacaa tgcgcgcagg atcatggcgg aggccgaggc cgccggcgtc
     4921 gcgatcgtgc tgccgatcga tggcgtcgtg gccgagacct tcgagcccca tgccgactgc
     4981 cgcaccgtgc cgatcgcggg ggtcggcgag gccgacatga tcctcgatat cggcccgggc
     5041 tcgatcaccg acatcaagga caggctcgcc agcgtgaagg tgctgctctg gaacgggccg
     5101 atgggcgcct tcgaggtccc gcccttcgac gtcggcacga ccgaggtggc gaaggccgcc
     5161 gccgtgctga cccaggcggg caagctcaag accgtggccg gcggtggcga cacggtggcc
     5221 gccctcgctc atgccggcgt ggtcgaccgc ttcacctatg tctcgacggc cggcggcgcc
     5281 ttcctcgaat ggctcgaggg caagaccctg ccgggcgtgg cggcgctcga aaccggctga
     5341 ggccaacacc gtgcggtcgg aagccccgcc gcttgccgtc ctcgtccacc accgcctcga
     5401 ggccgcggcg gcgatcgagc atgccaaggc ccgcggcgag gcgatcgagc tctgcatccc
     5461 cgccggcctc gccggcccgg cgttcgtcaa ggcgctggag gcgaccctgc agcacccgct
     5521 gaccgcgctc tgcgacgacg agccgggcct ggtcatggcc gccctgcgcg ccggcctgcg
     5581 gcggctcgtc ttcaccggcc cggaaaacgt gcgccagagg ctagccggca tggccgcggg
     5641 gctcggcgcc gaggtgctgg cgtcaccgcc cggcccgctc gtcgttcccg gccgcggcgg
     5701 ctcttggatg actccggccg tttgtgtaag ttccgcgccg accgatcccc cgctggccaa
     5761 agggactgcc ccatgagcga ccgcgtgaag accatcctct cctggtacga aggcgagaac
     5821 ccgggcgtga agggcaatct ctaccgcctc ttgatgcagg gtcgcctcgg cggcaccggc
     5881 aagctcgtca tcctgccggt cgaccagggc tacgagcacg gcccggcccg cagcttcgcc
     5941 aagaaccccg cggcctacga tccgcactat ctctatcagc tcgccatcga tgcggggctc
     6001 tccggctacg ccgccccgct cggaccgctc gagcaggggg ccgacacctt cgccggccag
     6061 gtgccgacca tcctcaagct gaacagcgcc aacagcctgc acagcggcat cggcgaccag
     6121 gcggtcacgg gctcggtcaa ggacgcgctg cggctgggct gcgtcggcgt cggcttcacc
     6181 atctatccgg gctcggaagc caattacggc atgatggaag agttccggga gctttccgcc
     6241 gaggccaagg ccaacggcct cgtctcggtg ctctggtcct atccgcgcgg cggcaatgtc
     6301 accaaggagg gcgagaccgc cctcgatatc gtggcctatg ccgcgcacat ggcggcgctg
     6361 ctcggcgcca ccatcatcaa ggtcaagcca cccaaggccg gcgtcgatct caaggccgcc
     6421 cagagctcct acgagaacgt gccgatggac agcctgaagg atcgcatcgc ccacgtcatg
     6481 cagtgctgct tcaacggcaa gcggctggtc gtcttctccg gcggcgaggc gaagagcacg
     6541 gacggcctgc tcggcgagat cagggagatc cgcgacggcg gcggctcggg ctcgatcgtc
     6601 ggccgcaaca gcttccagcg cccccgcgcc gaggcgctcg acctgctcgg caagatcatc
     6661 gagatctaca agcagggagc gtgagccgca gccgccgccg gacgggcagg aaggacccgg
     6721 acaccgagcc gggcaactgg gtcgacgtcg ccgaccaggg ctgtcggctg ctggtggcat
     6781 gcccggccgg gctggacccg gcacctttcc tcgcgatcgc cgccgtcgcc ggcctcgtcc
     6841 tgcccggcga gcccggggct gacacgatcg acgttgttcg ggccgtcggc cgcgccgcct
     6901 tcctggtcga tcgcccggac ctcgtgcggg cgctcggcgc cgacggcgtg ctgctcaacc
     6961 ggccgggcgc ggtcgggcca gcgcgcctat tgctcgacca gggcgagctg attggcgctg
     7021 cggtcggcgg cactcgccac gacgccatgg ttgcgggcga ggacggcgcg gactatgtgc
     7081 tgttcggcac gcccggcaag acaccggcgg acggcatcga gaacctcgca gcacacgtcg
     7141 cctggtggac cgggatcgcc gtcctgcctt gtgtcgtggc cggccggttc accgccgacg
     7201 acgtgcgccg cctcgccgcg gccggcgccg atttcctcct gccggacgtg gatgatgcgg
     7261 cgggcctcgc tgccctcgcc gccgtcctgc cggtccctga ccggtcctag aagggtagac
     7321 catgaagatc agcgtcaacg atgttcgccc gggccaggtg ctcgagcatc aggcgaagct
     7381 ctggcgggtg gtcaagaccg agcacgtcaa gcccggcaag ggtcccgcct acctgcaggt
     7441 cgagctcaag ggcgtcgacc acccgaccaa gctcaacgag cgcctgcgca ccgccgacac
     7501 catcgagcgg gtgcggctcg acgagaccga ctaccagttc ctctacgacg atggcgacca
     7561 gctccacttc atgaaccagg agagcttcga gcaggtggcc ctgcctaagg agctggtcgg
     7621 cgacggcgtc gtcttcctga aggagggcat gatcgtcacc gtgcagagct acgagggccg
     7681 gccgctctcg ctcgccctgc ccgagaccgt ggtctgcgag atcgtccagg ccgacccggt
     7741 ggtgaagggc cagaccgcgg ccgcctccta caagcctgcc atcctcgaca acggggtgcg
     7801 cgtcatggtc ccgccgcata tcgagatggg gacccgcgtc gtcgtcaaca ccgccgacag
     7861 cagctatgtc gaacgcgcga aggattgatc tgtggcgaga cgctccgcca attgcgagat
     7921 gatggtccgc gccgcgatca aggcggcgcg cggactggtg cgcgatttcg gcgaggtcga
     7981 gcagctccag gtcagcctga agggcccggc cgacttcgtc tccaatgccg accgccgcgc
     8041 cgagcgtatc ctcaaggagg agctcaggcg cgcccggccc gagttcggct tcctgatgga
     8101 ggaatccggc gaggaggtcg gctcgcagcc gcagaaccgt tggctggtcg atccgctgga
     8161 cggcaccacc aacttcctcc acggcctgcc gcatttcgcc gtctcggtgg cgctcgagca
     8221 gttcggcgag ctcaccaccg ccgtcgtcta cgacccgatc cgcgacgagc tcttctgggc
     8281 cgacaagggg ggcggcgcct atgtcaacga ccgccgcctg cgcgtctcgg cgcgcaagga
     8341 gctgggccag gcgctcgtcg gctgtggcct gccggtgcag acctggcccg gtcgcgagaa
     8401 gggcttcacc acccagatga atgccgtggc cgaccatgtc gccggcctgc gccgcctcgg
     8461 caccgcctcg ctcgatctcg cctgggtcgc cgccggccgc caggacgggt tctgggaata
     8521 cgggctcaag ccctgggaca tcgccgccgg catcctgctc gtgcgcgagg ccggcggtcg
     8581 cgtcggccgg ctcgaaggcg acgacgatct cctggccgag ggcacactgg tcgccgccac
     8641 cggcgggctc tttcccaagc ttaccgagct gcttcaggcc gccggctgag cctcccggcg
     8701 gaaggccggc acgaagagcg acccggccag caccacatag caggccgcgg ccgcggccag
     8761 cccttcgacc ggcacgcccc ggtcgaagac cgcaccgaag acgatgggcg aaagcgccga
     8821 gccgaagacc gagaacgcca tcgccaacga cctgatggcg cccagatgct cggtgccgta
     8881 gagctcggcc cagagcgcgt tgaccgcggt cgactggcca cccgaggcga gcccgaggaa
     8941 gatcatcatc aggatgccgg ccgcgaaggc gtcgctcgag gcgaggagca ggcagcctgt
     9001 ggcgagcagc agcggaaagg ccaggagcga ccgcgacgcg ccgaagcggt cgaccgccca
     9061 gccgctggcg agcgagctcg ccaccgagcc cagcgcatag atggtgaagg tggcggcgaa
     9121 gagcccgagc ggccagccct tggcaccgac gatgtgcacc tggtggaaga agatcccggt
     9181 gacgatgatc gatggcgcca gcacgcccgg cagaagcagc cagaaccgcg ggtcgcgcag
     9241 cacctcgcct tgccggcgac tcggcaccag cgagatggcc ggggcgacag cggttgaggc
     9301 cctcggacgt tcggcgcggg cgatgagccc gagcagcgcc ggcagcgcca gaacgaccag
     9361 tagcaccgcg aatccacgcc agccccatcg ccagccgagg ccgatggccg ccacggccag
     9421 gtgcggcatc aggatttcgc tgagggggaa gcccagcgtc acgatgctga tcgcccggcc
     9481 gcgtgacgcg gagaagcgct tggccatggt gctgatggcg atgtgcggcg ccatgccctg
     9541 cccggccagc ctgagcccat agaaggcgag cgccagtgtc agcgggccct gggcgaagct
     9601 caggaggagg caggccaggg cgaggcccag cacggtcgcc gatgtcagcc gggccaccgg
     9661 cacgcggtcg accagccggc cgagccagat cagcgtgacg gcgctggcca gcgtcgccgc
     9721 ggaataggtc gccccgaagg cgccgtggcc gaggccgaac tcggacctga tggcgccgcc
     9781 gaagacggcg atgaagaagg tctggccgaa gctggacgcg aagcagaaaa gtgccccgaa
     9841 gccgagcagc ggcccctgct gccgcaggag ctcgagaaag ccacgcatga tcgttccaag
     9901 ggagaacgcg gcgcagcata ggaccgcccg gccgccggtc aattgccgcc atggcgtgcc
     9961 agccccttcc ggccggcact ccctctggct agaacgacgg cgcccacggt ggcggcagcg
    10021 gaggcgacgg atgacggtga tcgaggcgct cgtggcagcg ctcggcgaag gcgtggtggc
    10081 ctttgatgcg gagagccgcg accggcagag ccgcgacctg agcaccgtgc cggcagcacg
    10141 gcctctggcc gtgctccggc cgcgcacgac ggcggaggtg gcgaccgccc tcggcatctg
    10201 tcacgcccac ggccagggcg tggtgcccca gggcgggcgc accggtgtct ccggcggtgc
    10261 agtgccacgc cccgccgaca tcgtcctttg cctggatcgg ctctccggca tcgaggcgat
    10321 cgacacggcc gccgccacca tgaccgtgct ggccggcacc ccgctcgaga ccgtccaggg
    10381 agcggcacgc gacgccggat tcctctgccc gctcgatctg ggtgcgcgcg gcacctgcgc
    10441 catcggcggc aatatcgcga ccaatgcggg cggcaaccgg gtcgtccgct atggcatgac
    10501 ccgccagatg gtgctcgggc tcgaggtggt cctggccgac ggcaccgtgc tcgacatgct
    10561 caacaccatg atcaagaaca atgccggccc cgacctgaag cagctcttca tcggcagcga
    10621 gggcagcctc ggcgtcatca cgcgcgccgt cctgaagctc gagccgctgc ccgtctcctc
    10681 cgccaccgcc ttttgcggcc tcgccggcct cgatgccgcc atcgcgttcc tcggtcgcgc
    10741 ccgctcgggc ctttcgggcc tgctctccag cttcgagatc atgtggccgg gctactacga
    10801 cctgatcatc ggcacgccgg ccggcagcgg tgcaacgggc gttcggccgc cgctcgaggg
    10861 ccgccacggc atgtatatcc tgatcgaggc gcagggcggc gagcccgccg ccgacgccga
    10921 ccgcttcgag cggttcctgg aggcctgtct cgaggatggc ctgatcgagg acgccgccat
    10981 cgcccagtcg ggcgaggccg cccgggcctt ctggggcgtg cgcgacgcgg tgatcgagtt
    11041 caatgcgctg ctcggccagc gcgtcgagtt cgatctcggc ctgcccatcg gccggatcgg
    11101 cacctgcacc cgtgccatcg aggcgagcct ccggcagagc tggcccgatg cgcggatcat
    11161 ctggtacggc catctcggcg acggcaacat ccacctgcag gtcgccgtcc cctccgttcg
    11221 gccgcatccc gaccatgcca tcgacgagat cgtctacgac cacctgcgcg agcacggcgg
    11281 caccatcacc gccgagcacg gcctcggcac gctgaaggcc ccctggctcg cccattgccg
    11341 cacacccgcc gagatcacgc tgatgcatgg cctcaaacgg accctcgacc ccatgggtat
    11401 cctcaacccg ggcaaggtgc tcgtcgacgg cgccacatga cccctgaatc gctcgtcgcc
    11461 ccgaccggac ggcccatccg cttgccttgg gactttggcc gcctgagcac cgtcgaacct
    11521 cgccgaggat cggtgctcct gtacaacgag ctggaacagg acctcggacg ggcgccgccg
    11581 agcgcgagca tcctgcagca tcacgtcgat cgcgccaccg accgtgtgac cgtgcagcgc
    11641 ctgcaatcga ccgagacggc gggtcatggc gatggccgcg caaggtgcac cgtacggccg
    11701 gctcgagacc gcacatgaaa tttcgtcaag cggttcgtgc ctgccggcca tcaagccggc
    11761 tcccttgccc gggcggcatg cgcaagctgg cgcagccgcg tcagcgactg catggcgctg
    11821 cggctgtcga acagcagctc gatgatgcgg attcgatcca tggtcgcccc catgggcccc
    11881 gcgcccggcc cggcagcttc ctccaggatg gcgcggaagc gctcgagggc cttggcgaag
    11941 acgaagtcga tcttcgtggc gagcccgatc cgcttcgcca tgagacgcat gttggccgcg
    12001 gccatggcat gagcctcgag cgcccggcag tccgccgatt tcgtgtcgtc gtcgagtgcc
    12061 ccgaaggcgt gcagcagagc gccgccgagc gacgcttcga ggcgtgcctc gatcagggtg
    12121 aacacttcgg ccttgagccg gcgcagctcg cgatggtcga acttgacctt ctgcgccacc
    12181 tcctggagcg actcgaggtt ggcgacgagg gtccgcaagg ggccgacgag cgccgagggt
    12241 ccggtcccgg ccggcagcag cgacaccgac cggaacaacc gcaccatctc gttctggcag
    12301 gcttcggcga tcatcgccga tgcctgctgc cgctcccggt cggcgagctc gagctcgccg
    12361 tccagcacgg cctcgatgat ggcgagcggc agctccgttc gagcgaccag gagctcggcc
    12421 gtgagcaaaa agcagtcccg cccctgggcg ctggcgagag cgagcagcgc gccgatccgg
    12481 gcccggccag cgggctccag agccagcatc ggcctgtccg gcaatgacca gatcgtggtg
    12541 accagggatt cggccaccgg catcaggttc tgcatgatgc gcagcggcgt gagcagctcg
    12601 cgaagctcgg tgtcccgcgt ctggcggcca cgggccagaa tcgcgtcggc ggcgaggccg
    12661 gcagcgggcc agatttcgcg accgacgtcg agcgccagcg cgctgtcgtg catcgacttg
    12721 ccggcgagct gcgacgccgc tccgcgccgg aggtccgggt cgatcgcctc cagcacgagc
    12781 tcgatcagcc ggccgaggcg atcacgcggc acccgcgtca tgccgggggt ccactcggcg
    12841 ccgtcgatca gcagctcctc cagcggcaaa acgacgagcc ggccgagggt catcggtcgc
    12901 ggcggtcgaa tgacggcaag acgtgcccga tgctcgtcga gcaactgatc gagcgagccg
    12961 cgcttcgcca gctggtcgac cacggcgacc agcttcagca aggtgcggtc atcggcgccg
    13021 tggagctgcg tcgccagcgc ctggaaggcc ttctcggcgc ccatcagccc actctccgac
    13081 gccatcgaca ccacgaccag ccgagccccg cctgcagtga tccaatcatc tcatgcaacc
    13141 ccggcacgaa gcgcttccgt ccgcgcacgg atgccgccca tgacgacgcc ggtccgccag
    13201 tcctcgtgca gcttgaccag acgcgaatgc accatcgtgc ccttcacccg catgacctcg
    13261 accgatctgc cctccgcctg ggtctgcgcg agcggcatca ggcgttggat ctgggcggcc
    13321 acggcgcgct gggctgcccg gccatcgcca ctcgcggaat cccagagggc aaggatctcg
    13381 ggccagccgt cgagcagcca gtcgggcagc gcgaaacgac ccttgagcgc gccccggtcg
    13441 tggcgccagg cacgaaccag ctcggggata tcctcgagcc gcacctgaca gtcggcgatc
    13501 aggggggcca ccttgttgct cgtccatccc gcgcaatcgg cgatctgctc ggcgcattcc
    13561 ttgctctcgc ccgtttcgag cgccgcccag tggcgcatgt cggcggcgaa tgggctcaag
    13621 cgttccagca cacgccggtg gcgcgtctcg agctcgccat cagcaaaacc gatggcggcg
    13681 gcatagaagg caatgtcgcc aaccgtatcg atcacctcgt cggcggtcat gccgaggcgc
    13741 gggctcagcg aggtgagtcg gcgcttgatc tcgagctgca cggcgcgccc ggtcccgccc
    13801 gagcgctgca gctcccgttc gtcgatcccg accgcgcgca ggaggcgcat cagcatcaga
    13861 tagttggtca ggttgacgat ggccgcgtcg cgctccgccg cccgtttcgc ggcagcggca
    13921 gcgtcgcggc cggccgcgcc ctccatcgcc acctcgcggg ccgcccgccg cacggtcgcc
    13981 ggcgtcacga cgtcgacgcc aaggagcttc tcgagcagca attcatcgtg cacggtgagc
    14041 tggcaataat cgcgcagcga gcgcagatcc atcacgtagt agcccagctg tcctgatgga
    14101 ttgggtgcca gaagctcgac gccgccggtc gcgaccagcc tcgccttcgc ccccgagatc
    14161 aggggcgtgg tgaagggcag gccgaccgag cgttcggcca gatcgagatg cctgtgctgc
    14221 cggtcgtccc ttgccatcga ttgagcagtc cgccgctggt cggcccgtca ctggtagatg
    14281 gcgcgcactc taggccggtg cttcttgcca tccggttaac ggcgtgacgg gcaatgcgct
    14341 ggcctactgg acgaagcggg gcatcagcca gcgcgggagg gcgaggatga tgtcgggaac
    14401 gacgatgatg agcatcagga tgccgatcat cggcaggagg atgatcgcga catccctgag
    14461 cgccctgatc agcttgatct cgccgatggc cgccgcgatc aggaggcaga ggccataagg
    14521 cggcgtgacc aggccgaagg cgaggctgac gactccgatg atggcgaagt ggatcggatg
    14581 catgccgaca tgctcgacca cggggtagag caccgtgccg aggatgatga tcgccggaat
    14641 ggcatcgatg aacatgccga tcagcaggaa ggccagcgcg accagcatgc cggtgccggt
    14701 gacgccgagg cctagcgtct cgatctgcgc caccagcgct gccgggatct tgaaataggc
    14761 gagcgcccag ccgaacgccg aggcggtgcc gatgcagaag agcgacagcg ccgcgaaacg
    14821 cgcactgtcg tagaggatcg tcggcagttg ctggaagctg accgagcgat agatgaagag
    14881 cccgaggatc agcgagtaga tcaccgccac caccgaggcc tcggtcgggg tgaacaggcc
    14941 gcccacgatg ccaccgacga tgatggccgg cgtcatcagg gccaggagcg agccaacgaa
    15001 agcggcggcg agctccttga gcgtgctgcg cgcgtagatc ggatagccgc gccttttcgc
    15061 atagacatag acggttgcca tctgcgccac ggcgatcagc acgccgggca cgacgccggc
    15121 gaggaacagg ccgccgatcg agaccgacat gaggccgccc cagacgacca tcaggatcga
    15181 gggcgggatg atcacgccca tgaccgagga gcaggcagtg atcgccacgg cgaagctcgc
    15241 gtcgtagccc tgcttcttca tctgcgggat gaggagcgag ccgatgccgg cggcatccgc
    15301 ggtcgagctc ccggagatgc cggcaaagag catcgagacg acgacgttga cgtggccgag
    15361 cccgcccggc agatggccga ccagggaacg cgcgaacgcg atcagccggt cggtgatccc
    15421 ggccccgttc atcaggttgg cggcgagcag gaagaacggc acggccagca ggacgaaggc
    15481 attgtagctc ttgaacatct cccgcaggag caggatcggc gtcagacgct cgtcgaagaa
    15541 gaagaccggc accacggcga ggccgagcgc gaaggcgacc ggcacgcgca gcaccacgag
    15601 cacgccgaac aggccgaaga ggatggccgc gacctcgccc gggctcatcc ggcacccacc
    15661 gtccgcggac gaggggggtc gaccatgccg agatcgacga ggatgcgctc gccaacgaag
    15721 agcagccagg tcgccccggc ccacggccag gcgatgtaga tcgagagcat cggcaggccg
    15781 gcgatctcgc tgctctgccg cgagccgaga atggcgaaat cccagcccca gatcaggaag
    15841 atgacgccga cagcgccaat cgtgagattg accaggagcc gtgccgcgac gcggcccgct
    15901 ttggtcttcg gctcgggcaa gaggtcgacg tcgaaatgcg tgccgtcgcg cacggcgatg
    15961 ccggcgccca ccatgatcat ccagatgaag gcgaagcgcg cgatctcctc ggtccagatg
    16021 tagcgcggga tcaggcccgt gtaacgcgac aggacctgca tggtcaccgg cacgatgagg
    16081 acggcgatca gcagcgtcag cagcagctgc aggacgcgat agtagccgtc gacgaagcgg
    16141 cgcatcaaaa ggtgcggaac atgcgtggcg gcgggcaggc ggcgctggtc gccgcccgcc
    16201 cgtcgaccct actggacgga gacgatattg gcgtagacgt cgctcgcccc gagctcctcg
    16261 gcataggcct gcatcaccgg ggccgcgagc tcgagcagct tcgcccgatc ggtgaactcg
    16321 tagggcgtca gccagccctt ctcctccatc gccttcagct tctcggcatc ctggccgctc
    16381 tcgatctcgc gaccgagctt gccggccgcc ttgccggact cgacgatgca ggcctgcagc
    16441 tcggggtcga gccgctcgaa cgtcgcgttc gagaagccca agggccgcac ggtgatcgcg
    16501 tgctgggtca aggcgagatc ggggccgacc tcgtagaact tcatctgctc gatgcccgct
    16561 gcctcgttct cggccgcggc gatcacgccg gtctggatag cgttgtagac ctcgttgtag
    16621 gcgatcaccg tcggggccgc acccagcgca tcgaagaccc gtgtccagat gggagccccc
    16681 tgcatgcgga tcggcaggtc cttgagctcc tccatattgc gcaccggcct gttggcgaag
    16741 acgttgcgcg tgccaccacc ggcatagccg atgatcagca cattggcctt ctccaggatg
    16801 tcgtccgcga tcgggtcgag cgcgcccgcg tccatcacct tgttccagtg atcgagatca
    16861 cggaacagga acggcatgtc catcaaaggc gccatctgcg agaaggtcga ggcgtgcgcc
    16921 ggcgagaaca ccgcgtaatc aacggcgatg ccctggctca tgttctcgaa ataatccttc
    16981 tccagaccga gctcggaatt caggtgcagg tcgaaggtga cgtcaccgga atagcattcg
    17041 ccgaccagcc gctcgaactc gcgcatggtc ttggtgaagg catggttctc gtcgaactgg
    17101 ctggcgccga ccagggtcac gccctgcgcc gaagccgtgc cggccagcgc cagcagggcg
    17161 acgccaccgg cgagcatccg tgtgatccgg ttcatgactg cactcccttg tgcgttctct
    17221 cgttggtcag atctctctga tcgcagaaag tcgtacggct gtcacgggcg tagggaccgg
    17281 tcacggcaac gatcgccctt cggacggctg cgtggcggat ggctcgggca agagctgcgg
    17341 atagccgggt gcggccgacg ccttcacgtg cacgtcctgc tgcggatagg ggatcgagat
    17401 cccggccgcg tcgaagctct ccttgatccg ctgcgtcagg tcccaataga cggtccaata
    17461 gtcgcctgtc ttcgtccagg gacggcagat gaagttgacg gagctgtccg ccagctcgtt
    17521 cacccggatg accggcgccg gctcggcgag caccagggga tgctgcctga ccgcctcgtg
    17581 catgacgcgg atcgcgtccg ggatggagtc gtcgtacgat atgccgaaca ccaggtcgac
    17641 gcggcgcgtg gcgctggcgg tgacgttggt gatcacgttg ccccagacgt tcttgttcgg
    17701 aatgacgatc aactggttgt cgggcgtggt gacggtcgtc gccacgatgc tcacggactt
    17761 caccgtgccg gcgacgccgc cgacgtcgac ataatcgccc tgatcgaagg gcttgttgat
    17821 catgatcatc aggccgctcg cgagattgcc cagcgtgtcc tggaacgcga acgccatgat
    17881 gaaggaggca ccgccaatga gcgcgaagac gggcgagacg tcgacgccga gcgccgacag
    17941 caccaccatg atgccgatcg ccagaaccag ccagtagatc acggcgatca ggaaggcctg
    18001 aagcagcttc gacaggttcg gaatgcgtcc gatatagcgc cccacgaccc ggcgcacgat
    18061 ccgcgccacg atcagcagga ccaggaacgc gccgacgacg atggcgatgt cgaggccgag
    18121 ctcgatgccg ccgtccctgg cgaccagcca ggcgagcgcc tggttcatca gggtccggaa
    18181 gtcggcggtc cgggtctcct cgatgatgat cgagctcttg taggcgcgat agtcggcgac
    18241 ggccgcctcg tcgccgccct tcttttccca ggcggccacg accttgtcgt aattgttgaa
    18301 gagcttcttg cgctcggcca cgagctcggt cagctcatcg cgcttcgcct cggcgacatt
    18361 gccctcgctc tgcgagatct cgacctgaag gcgggcgacc tcttccgtct tggccttgac
    18421 gatctgcagc cattccccgg ccagcgccgc gagctcgcct ttggtcagcg gcacgaggcg
    18481 gtattcgagc acctgggggt cgacggccgg gtcgagcagc tcttccggca gctccggccc
    18541 ctcgtcttcc gccaccgctt cggctggcgc cgcttcggca gcggcctcgt ccgtttgcgc
    18601 aagagcgggc ggcggcggga cgaaggcgtg cagcgcgagc aggatcagga ttgccaggca
    18661 caaggcagcg cccgatccac gcgtggtcca ggccaatgtc atatgcggtt ccttcccagg
    18721 tttcatgccg aggccccggg gcagggtccg aattcgacgt ttcgacggca cgcttcgtcg
    18781 gcggcaacga gccgcggctt ccgaccgcac gccttcgatg atcgcagcca atgtgaaagt
    18841 cgaatgggtg cctgcacccc ggaatgtggt cgcgtgttaa ttcatgcgag ccgatttggc
    18901 cacccaccct tgcggttcac ccgaccgggc agagcgcctt tgcgccgcag gccgtcgcgg
    18961 ccgatcgggt gaaccggcgg aaagccgggt ccgactcagg cccgatccgg caccgctggg
    19021 gcccggttcg tcgaggtggc ggtttcgaac gcctcgagcg tctccctgac cttggccacg
    19081 atcgccggcg cgtcgagccc cgcctcggcg agctggccgg tcgggctgcc ctgctcgagg
    19141 aaacggtcgg gcaacatcat cgtgcgcaac cgcgcatggg caccatccag gagcccatgc
    19201 tccaggagga aatgctggac gtgtgccgcg aatccgccga tcgagccttc ctcgacggtg
    19261 atcagcagcg catggccacg ggccagctca accagcagcg ccgcatcgag cggcttggcg
    19321 aagcgtgcgt cggcaaccgt tgtcgagatg cccatcgcga gcagctcgtc cgccgccttc
    19381 aggcagtcct gcagccttgt gccgagcgag aggagcgcta cccggttgcc gcgacggacc
    19441 acccggccct tgccgatctc gagcacgctg ccacgctccg gcagctccag gccgaccccc
    19501 tcgccacgcg ggtagcgaaa cgcgatcggg cccgagtcgt gagccgccgc ggtcgccacc
    19561 atgtggcaga gctccacctc gtccgccgcc gccatcacca ccatgttggg cagcgtcgcc
    19621 agataggtga tgtcgaacgc acccgcatgg gtcgagccgt ccgcccccac cagaccggcc
    19681 cgatcgatgg caaagcgcac cggcaggttc tggatcgcca cgtcgtgcac gacctggtca
    19741 taggcgcgct gcaggaaggt cgaatagatg gcgcagaagg gcttcaggcc ctcgcaggca
    19801 aggcccgccg caaaggtcac cgcgtgctgc tcggcaatcc cgacatcgta gacccggccg
    19861 ggaaagcgct gcctgagccg gtcgagtccg gtgcccgagg gcatcgccgc ggtgaccgcc
    19921 acgatcctgg gatcggcctc ggcctcgcgg gtcagcgcct gcgcgaagac accggtgtag
    19981 gagggcgcat tcgacctgct cttgacctgg gcgccggtgg cgacgtcgaa gcgagcgaca
    20041 ccgtgatact tgtcggcggc ggcttcggcc ggcggatagc ccttgccctt ctgggtcacc
    20101 acgtggatca ggaccggccc gcgatcggga tcgtcgcggg cgttctcgag caccggcagg
    20161 aggtggtcga gattgtgacc gtcgagggga ccgatataga aaaagccgag ctcctcgaag
    20221 agcgtgccgc cggtcgccat gccgcgggcg tattcctcgg cacgctgggc ggcgcgctgc
    20281 aggggccgcg ggaaatggct cgcgagctgc ttcgccacat ggcggaagct gcgataggtg
    20341 tggctcgaga ccatccggct cagataggcg ctcatcgccc ctaccggcgg ggcgatcgac
    20401 atgtcgttgt cgttgaggat gataatcagc cgctggcgga gcgccccggt attgttcatc
    20461 gcctcgtagg ccatgccggc gctcatcgcc ccgtcgccga tgaccgccac gacattgcgg
    20521 tccctgccct cgagctggtc ggcaaccgcc atgccgagcc cggccgagat ggaggtcgag
    20581 ctgtgcgccg ccccgaacgg gtcgtagacg ctctcgctgc gtcgcgtgaa gcccgagatg
    20641 ccccccggct ggcgcaacgt gcggatgcga tcccggcgcc cggtcaggat cttgtgcggg
    20701 tagcattgat gaccgacatc ccagatgagg cggtcatagg gcgtgtcgaa gacgtagtgc
    20761 agcgccactg tcagctcgac cacgccgagc ccggcgccga gatggccgcc ggtcaccgag
    20821 accgcgtcga tggtctcctg gcgcagctcg ctggcgagct ggccgagcga cgatcgctgc
    20881 agccggcgga gatcgcgggg ctcgttgatc tggtcgagaa gcggggtctg ggtcatggtg
    20941 cgcgttcgag tcccggcagc gttcagtggg tacggtggat cacgaagccg aagagtgctt
    21001 cgaggacgtt tgcgcgtccg gcaaaaccgg cgagcaggcc cgccgcctcc gcctcgaggc
    21061 gccgcagctc cgcctcggcg ccggcgggcc cgagggcggt gatgaaggtg gccttgccct
    21121 ggccggcatc gtggccgaca tccttgccga ccagtgccgc atcaccacgc aggtcgagca
    21181 ggtcgtcgct gatctggaag gcgagaccga gccgtccgcc ataggcgagc agagcctgcc
    21241 ggtcgacgtc gccggcccgg ccgagcaggc agccggcctc gcaggagaaa cggatcaacg
    21301 ccccggtctt caaggcctgg agctcgcgga tgccggtgag atcgagggcc agcctttcgg
    21361 cttcgaggtc gatcatctgg ccgccgcaca tcccggccgg gccgccagcc cgcgagaggc
    21421 cgaggacgag ggcgcgcgag gtcgcatcgt cgatgccggt cacgccccgg gcgagcagct
    21481 cgaacgccag ggcctgcaag gcgtcgccgg cgagaatcgc cgtcgcctcg tcgaaagcct
    21541 tgtggcagct cggccgtccg cgccgcatgg ccgcgtcatc catcgccggc aaatcgtcgt
    21601 ggaccaggga ataggcgtgc aggagctcga tggcgccacc aacggcgagg ctctgctcgg
    21661 cgggcacttc aaagagatcg cccgtcgcct tgaccaggaa ggcccgcagc cgcttgccgc
    21721 caccgagcgc cgcgtaacgc atcgcccgga cgagcgccgc tgtggcaccc tccggccgcg
    21781 gcagcgcctc gtcgagccag gtttcgaccg ccgccgcatg gtcccgcagc gcccgctcca
    21841 ccgccggccc gtccatcagc agggcttccg gctcatgtat cgtcctgggc ctcgaggccg
    21901 accttgccat tgcggtcgaa ggtgatcttc tcgacccgaa gctgcgcctc gcgcagcttc
    21961 gtggcgcaat gctggcggag cgctgtgcct cgctcgtagg ccgcgatcga ggcttcgaga
    22021 tcgagctggc cgcgctccag ccgctgcacg atcgcctcga gctcggccag cgccacctcg
    22081 aagctcatcg acccgatcgc cggctcggcc gccgcggtcg cttcctgcgg ttcctgtttc
    22141 gccatgctca acccaatgct caccgatgcc tgtcttagcg accacggccg ggcttgcaaa
    22201 cggcgcccgg cggaaatgcc acgtcctagg cggtcgtccc ggccagcccc tcgagatggg
    22261 ccagaacgct ccgcgccagc gcatccgggt cgtagccgcc ctcgagcacc gagacgatcc
    22321 gtccctgact gtggcgctcg gcaacctcga cgagctttcc cgtgacccag gcgaaatcgg
    22381 cctcggtcag ccgcatctgc gccaaaggat cggcctcgtg cgcgtcgaac cccgccgaga
    22441 tcatcagcag ctccggccgg aacgcgtcga ggcgctgcag gatggttccc tcgaccttct
    22501 cccgccacag ggccgagccg gtgccggcgg cgcaggggac gttgacgaca ttgtcgtggc
    22561 tcccccgctc ggcggcggca ccggtgcccg gatagagcgg catctggtgg gtggaaagat
    22621 agagggtcag aggatcgtcc cagaagatcg cctgggtgcc gttgccgtga tgcacatcga
    22681 agtcgtagat ggcgatccgc tcgatgccgt gaaccgcccg cgcctgcagc gccgccaccg
    22741 ccaccgtgtc gaaaaggcag aaccccatgg cctgctgcgg ctcggcgtga tggccgggcg
    22801 gccgcatggc gatgaaggca cgcctggcct cgaccccgag caccgcgtcg acggcagcac
    22861 agccggcccc ggcggcgcgc agcgccgcct cggctgagcc actggacatc agcgtgtcgg
    22921 gatcgagccg cacatagcca tccatcggct cggcggcgag gatacgctca gggtatggtg
    22981 ccggatgcgc ccgctcgatc tgcgccagcg tcgcctgcgg cgcttcgcgg cgcaccagga
    23041 aggccggccg ctcggccgcc agcagcgcct cgatcgcccg aagtcgcccg gcgttctccg
    23101 gatggcccgg gccggcatcg tgtcgaagtg ccgatggatg ggtgtagtag agaacgttca
    23161 agttgcctcc ctgatcggcg ccgctcaggc tgcgcggggc tggacatcac gctggatcgc
    23221 ctcgcccttc tcgcactgca ccagcgagag atcatgggct gtctgcaggt tcagccagaa
    23281 ctggggagtg gtgccgaaat agcgggcgag gcgcaaggcc gtgtcggccg ttatggacct
    23341 cggctcgcgc tcgcggatga tcgccgtgat ccgcgtcgcc ggcaccctga gcgccagggc
    23401 cagtgcgttg gcgctcaggc cgagcggcac cagaaattcc tcacgcaaga cctcacccgg
    23461 atgggtccgc acacggttca ttggtccacc tcaatgatag tcgacgacct cgaccgcctc
    23521 cggcccgtcg ttccagacga agcagatgcg ccattgcccg ttgacgcgga tcgaccactg
    23581 accgtcacgg tcacctttca gcttctcccg acgattgccc ggcggttggc gcagatcgtc
    23641 cagctccatt gccgcatcca gcatgtcgag cttgcaggcg agaaccgccg acatcgaacg
    23701 ccagacagga gcgaactcac ctcgaaacgc cgccgccgac cgcttgtccg caaagctccg
    23761 gatcatgcag tcacgtagta cgtaacgcgt tgcatgtcaa gcgttcaacg tcacgcgtca
    23821 aaaccttctt cacacctacg ccgccagacg cgccagctcg tccaccacct tcttcgccgc
    23881 cttgaggcgg tcggcgggag tcgtcgtgtc ctgcatgacc accagcttgt gatcgggccg
    23941 cacccgcatc cggcccttgc tgccggcgat gtgctggatc agccgctccg gctcggcgaa
    24001 gcggttctcg cggaaggcca ggaccacgcc cttggggccg acatcgagct tctgcacgtt
    24061 ggccttgcgg cagagctgct tgatggcgac gatctgcagc aggtgctcgg tctcggccgg
    24121 cagggagccg aagcggtcga tgagctcggc ggcgaagccc tcgatgtcct cgggctcctc
    24181 gaggctggcc agcctccggt acatggcgag gcgcagatcg agatcggcga tgtagctctc
    24241 cggcatcagg gccgccgcgt cgatggtgat gtgcggcgtc cactcggtcg tggcctcggc
    24301 atcgccagtg gcgcgcaggg ccgccacggc ctcctgcagc atgtggttgt agagctcgaa
    24361 gccgacctcc cggatctgcc cggactgctc gtcgcccagg aggttgccgg cgccgcggat
    24421 gtcgagatcg tggctggcga gctggaagcc ggcgccgagc ccttccagcg actggatgac
    24481 gtgcaggcgc ttctccgccg tctcctgcag gatcttgttc gccggcacgg tgaagtaggc
    24541 gtagccccgg accttggagc ggcccacccg gcccttgagc tggtagagct gcgacagccc
    24601 gaagagatcg gcacggtgga cgatcagcgt gttggccgtc gggatgtcga gtccgctctc
    24661 gacgatgttg gtagcgagca gcacgtcgag ctgacggtcg tagaaggcgc tcatcaccgc
    24721 ctccagatcc ttcgccggaa gctggccgtt ggcgatcgcg accctgacct cgggcaccag
    24781 ccgctcgagc tcctccttga gcttcggctg gtccttgatc ctggggcaga catagaagac
    24841 ctgcccgccc cgatgatact cgcgcaggat cgcctcgcgc agcaccacgg gatcgaccgg
    24901 catgacgaag ctgcgcaccg cgaggcggtc gaccggcggc gtggcgatga tcgacagatc
    24961 cttcattccg cccaaagcca tgtgcagcgt gcgcggaatg ggtgtggcgg tcatcgtcag
    25021 gacatgcacc tcggcgcgga gctgcttcag gcgctccttg tggctgacgc cgaaatgctg
    25081 ctcctcgtcg accacgacga ggccgagatt cctgaagctc accgacttgg cgagcagggc
    25141 atgggtgccg atgacgatgt cgaccgtccc gtcggccagc cccttcttga cctcgttcgc
    25201 ctgcctggcg gtgacgaagc gcgagatctg ctccacccgc aacggcaggc ccttgaaccg
    25261 ctccccgaag ttcttgaagt gctggcgggc gagcagcgtg gtcggcgcca ggatggcgac
    25321 ctgcttgccc gacatggcgg cgacgaacgc cgcacgcagc gccacctccg tcttgccgaa
    25381 gccgacatcg ccgcagagca gccggtccat cggctgcccg gaagccagat cctcgagcac
    25441 cgtctggatg gcacgaagct ggtcctcggt ctcgtcgaag ggaaagcgtg ccgcgaactc
    25501 ctcgtagagc cccggggcga gggtcagggg ctcgcccttg cgcagcgccc gggcggcggc
    25561 gatcctgatc agctcgtcgg cgagctcctt gatccgctgc ttgatccggg ccttgcggtt
    25621 ctgccagccc acgccgccca gcttgtcgag ctgcacctcc tgctcggcat tgccgtagcg
    25681 ggtcaggatg tcgagattct cgaccggcac gaagagcttg tcgccaccgg catagagcag
    25741 cttcaggcag tcgtgcgcgg cgccgccgat ctcgagcgtc accagcccct cgaagcggcc
    25801 gatgccgtgc tcggcatgca ccacgagatc gttctcggcg agcgtgccca cgtcctggat
    25861 gaacttgtcc gcggccttgg cacgccgcgc ctgccgccgg gtcagccgct cgccgagcag
    25921 atcctgctcg ccgagcaggc agagccccgg ggcgctgacg ccggtctcga gcggcagcac
    25981 cgccagatgc gtggcacccg ggacgagctc ggccagggcc tcgatgcgca agggtggctc
    26041 gagcccgtga tcgaccagca cctgctggag ccggtcggcg gcgccaaccc cgtgcacggt
    26101 gatgatcggg ctctcgccgg cagccttgag ctcgcgcaga tgggcgacga tggcgtcgaa
    26161 gagattgatc gcgcgatcgc tccgttcgcg cgcgaagtcc cgccccggcc ggccacccag
    26221 atcgcgcaag cccgccacgc ccgagggcgg cacgggcggc gggccgaaga tcgagaagag
    26281 ccaggccggc cgcccggcga gccggcgccg gaggctctcc tcgtccagat agagcatgtg
    26341 cggcggcagg gcgcggtacg ggatcgagcc gaaggcaccg gacttggcgg ccggcgcggt
    26401 ccgcgcctcg aaatgctcgc cgatcgccgc ctgccgcgcc gccaccgcat cctcggccag
    26461 atggtcgaag gcaacctcga agccgccgtc gagatagtcg aagaccgtca ccagcttttc
    26521 gtggaagagc ggcagccagt gctccatgcc ggggaaatgc cgcccggccg cgaccgattc
    26581 gagcagggga tcctcggtca ccgtcccgaa ctgccgcaga tagccggcgc ggaatcgagc
    26641 cgcggccgca tcgtcgagca ggacctcgct caccggcccg agctccactg tcttcagccg
    26701 gtcggtggtg cgctgggtcg ccggatcgaa gcggcggatc gattcgagcg tcgagccgaa
    26761 gaaatcgagc cggacgggtt gctcggcccc ggtcgggaag aggtcgatca gcccgcccct
    26821 gaccgcatag tcgcccgggt cgaccacggt gccgacgcgc tggtagccgt tccgctcgag
    26881 atgggcgagc agcccgtcgc gctcgacccg ggccgaggcc ctgagctgga ggtggctggc
    26941 gcgcaccgcg tccggtggcg gcaggagctg ggtcagggcg ttggccgaga ccagcaccaa
    27001 gagcttccgc cggccccgct cccgggccag ccggtgcagg gtggcgagcc gctcggccac
    27061 caccgtcgca ttgggcgaga cccggtcata aggcaggcag tcccaggccg gcagcgtcag
    27121 gatctcgatc ccgggcgcaa agaaccggac gagggcggcg agggccgtgc tgcgctgcgc
    27181 gtcgcgggtg acgtagagca gcgtgtcggt ggcgccgctg tccagccgct cggcgacgaa
    27241 acgcgcctcg aggccgccca gcgcgttgcc gagcaggatt cgctcgccgg cagcgccggc
    27301 ggtgtcgacg gggtcgagaa gcgtggcagt ggtcaactga ccggctcgaa gctctgcagg
    27361 agccgaaaga ccccgtggtc gtgccgggcc gggacgtgcc gggcaccact cagccaggcc
    27421 cagacctcct ggtccggctc gtcgagcagc gcctcgtact ggtcgagctc ggtgccgtcg
    27481 aggccggcga gatgccggtc ggcgaattgg ccgaaatgca ggtcgacctc cttgaagccg
    27541 cgatagcggc tgcggtgcgc cagccgtttc agccgcgtgg tccgggcgtc gctcatgctt
    27601 gcaccacctc cggggccgac cgacaatgac ggcgacccga caacgggaaa agcgtccgaa
    27661 ccccgacagg ggccgaacga catcacgcgc aagggatcta gtcgatatgg ccgaaccggc
    27721 caagggcaca ggcggcgaaa tggcccgccc ggccgagctc tatgccctct ttcgcccgct
    27781 cgagagcctg cccggcgtcg gcccgaaggt ggcccggctc tgcgccaggc tgctcggccg
    27841 gagcgagccg cgcctgctcg acctcctctt caccccaccg gtgcgccggc gcgacatcgc
    27901 cgaccggcgg cgactcgagc ccgaggacga gggccggctc gtcgccctcg agctcgtcac
    27961 cgaggagcat cgaccaggcc atggccggtc accctatcgg attcgctgca ccgccgagcg
    28021 ccagaacgtg acgctggtct tctttcatgc gcggcagccc tggctggagc gccgcttccc
    28081 gcccgaccga accgtccggg tgatcggccg gccggagctc ttcaatgacc agtggcagat
    28141 cgtccatccg gagccggcga gcgagggcgg ccgggacgcc accgtctatc gtctgatcga
    28201 gggcctgggt gcgctgcgcc tcaagcggct gatcgacgat gccctggccg aggtggggga
    28261 gctgcccgaa tggcacgatc ccgcgaccct cgaggcccac gacctgccgc cctggcgggc
    28321 agcgctggcg gtgctgcacg gccgcgtccc gggcgaagtg ccactcgacg acgcggctat
    28381 cgagcgcgcc cgccgccgcc tggcgctcga cgagctgacc gccacccagc tcgccctggc
    28441 gctggtccgc cgcagccgcg accgggtgcc ggctccgccg ctcgccggcc cgagcccgag
    28501 gacggaggcc atgctggcct ccctgccctt caccccgacc ggcgcacaag cccgggcgat
    28561 cggggagatc gcgaccgatc tggcccgccc cattgcgatg gcgcgcctgc tcatgggcga
    28621 cgtcggcagc ggcaagacac tggtggccct ggccgcaatg ctccgcgcca ccgatcatgg
    28681 gcgccaggcg gtgatgatgg cgccgaccga agtgctggcc cgccagcacg cccggagtct
    28741 cggcgcgttg gccgagccgc tcggcctcga aatcgacctc ctgatcggcg gcgacagtgc
    28801 tgccgaccgg cgccgcgcgc ttgagcggat cgccagcggc gaagcggcga tcaccgtcgg
    28861 cacccacgcg ctcttccagg agggcgtcac cttcaacaac ctggcgctcg ccgtcatcga
    28921 cgagcagcac cgcttcggcg tggcccagcg tctcggcctc gtcgccaaga gccacgcgac
    28981 gcacctcctg ctgatgaccg cgaccccgat cccgcgcagc ctggtcctca gcgcctatgg
    29041 cgatgtcgag acctcgcggc tcgacgagaa gccgcccggc cgccagccca tcgtcaccgc
    29101 cgccctgccg cgtcgccggc tggaggaggt gctggagcgc gtcgccgccg cgatcggccg
    29161 cgacgagcgg atctactgga tctgcccgag cgtggctgag gcgacgaccg acggcccggc
    29221 cgacgacgac accggcagga tcgccgccga aacccgcttc ggggagctcc aggggcgttt
    29281 cggcaaaacc gtcggcctcg tccacggcaa gctcaaaacg gccgagaaga cggccgcgat
    29341 gcaggccttc gccagcggcc ggacgcgact cctcgtcgcc accacggtca tcgaggtcgg
    29401 cgtcgatgtg ccggaggcaa cggtcatcgt catcgacggc gcccagcatt tcggcctcgc
    29461 gcagctccac cagctgcgcg gccgggtcgg ccggggcagc cggccctcgg cctgtgttct
    29521 cctctacgat ccgccgctcg gcgaggcagc cagagcgcgg ctcgccatcc tgcgcgagag
    29581 cgaggacggt tttcgcctcg ccgaggagga tctcaggctg cgcgggccgg gcgaggtgct
    29641 cggcgtgcgg cagagcggcc tgcccgagct ccgctttgcc gatctcgccg acgatggcga
    29701 tctggtcgcc accgcccatg acggtgtggc cgtcgccctg gcgcagaacc aggcgctcgc
    29761 cggtgagcgt gggcgcgcgc tccgactcct cctgcacctc ttcgagcgtt acgaggcggt
    29821 gggctgcctc gccgccggtt gagccatcgc gacattttcc aaacgcagtg tggcgccacc
    29881 gtcgattgca cgtcgccgga tgcgtgatat aagcccgcga tcatcgccaa cagggaggat
    29941 cgatcatgcg gtcggtcgcg cccgggtttc tcggactctt tcatgccgcc cgccgtcctc
    30001 gtgctcaacg ggccgaacct caatcttctg ggtcggcgcg agcctgccgt ctacggtcat
    30061 gagaccctgg ccgatctcga ggccgcctgc gttgcggcgg gcagcgatct cggcctcgct
    30121 gtcacctgcc gccagagcaa ccacgagggc gagctcatcg actggattca cgcggcccgc
    30181 gagacgacgg cggcgatcct gatcaatgcc ggtgcctact cgcacacctc gcaggcgatc
    30241 cccgacgcgc tccgggcgta tcccgggatc atcgtcgagc tgcacctctc gaacatctac
    30301 gcgcgcgagg cgtttcgcca ccattcgcac atctcggcca ccgccacggc cgtgatctgc
    30361 gggctcggca gccagggcta tcacctggcg ctccgggcgg tggccgagcg gctggccgcg
    30421 tccgcgcaac acccggacgg ctgacatcat gggcaagctc aacatcgaca ccgactacgt
    30481 cgcggcactc gccgaactgc tgcagcgaac gggactcacc gagatcgaga tcagcgaggg
    30541 cgatgcccgt gtccgtgtcg tcaagaagcc ggcgccggtc tacgaagccc atgtggcgcc
    30601 ccgggccgcg gcaccggtcc tggcggacag cgagccgacc acggcaccgg ccaacgagcc
    30661 agcgcacccg gggacagtga cgtcgccgat ggtcggcacc gtctatctct atccggagcc
    30721 gggcgccgac cccttcgtgg cacctggcca gacggtgcgt gagggcgaga cgctgctgat
    30781 catcgaggca atgaaggtga tgaaccagat ccgcgccccc cgcagcggcc gcatcgcacg
    30841 gatcttcgtc gacaacgcga caccggtcga gttcggcgag ccgctcctga tcatcgagtg
    30901 agcgcgagcg tggtcgccat ccagccgatg aagaaggtgc tgatcgccaa tcgcggcgag
    30961 atcgccctgc ggattctgcg cacctgccac gagatgggca tcggcaccgt cgccgtgcac
    31021 tcgaccgccg atgccaatgc catgcatgtg cgcctcgccg acgagagcgt gtgcatcggc
    31081 ccgcccgggg cgggcgaatc ctacctgaac gccgcagcga tcctctcggc tgccgaaatc
    31141 accggggccg agggcatcca tccgggctac ggcttcctcg ccgagaacgc cgacttcgcc
    31201 cagatggtgg tcgagcacgg gttcaccttc atcggcccgg atccgggcca catccggatg
    31261 atgggcgaca aggtggtggc caaggcgagc gtcgccgccg ccggcatccc gaccgtgccc
    31321 ggcaccgatg gtgccgtgac cgaggatgcg atggcgttca ccgcggcgga cacgatcggc
    31381 tatcccgtcc tgatcaaggc ggtagccggc ggcggcgggc gcggcatgca cgtcgtgcgc
    31441 agccgtgcgg agctgggtgc ggcactccgc aacgcccgca acgaagcccg cgccgggttc
    31501 ggcgacgagc gggtctatct cgagaagtat ctcgaccatc cgcggcatat cgagatccag
    31561 gtgatggccg accggcacgg caatgtcgtg catctgggcg agcgcgactg ctccctgcag
    31621 cggcgccacc agaagctgat cgaggagtcg ccctcgccgg tgatcaccga cagcgagcgc
    31681 cggacgatcg gcgagaccgt cgtcgaggcg ctcggcaagc tcggctatct gggcgccggc
    31741 acgatcgagt tcctttatca ggatggccgg ttctacttca tcgagatgaa cacccgcatc
    31801 caggtcgagc acccggtgac cgaaatggtg accggcatcg acctcgtgcg cgagcagctc
    31861 accgtcgcca tgggccatcc gttgagcttc acgcagaagc aagttcggct cgaggggcat
    31921 gcgatcgaat gccggatcaa cgccgaggac ccgcagaccc tgctgccgca gcccggccgg
    31981 atcgtcgcct accacccgcc gggcgggccc ggtgtgcggg tcgattcggc catctacagc
    32041 ggctacaccg tgcccccctt ctacgacagc ctgatcgcca agctgatcgt ccacgacaag
    32101 gaccgcgagc gctgcatcgc ccggctcgag cgcagcctcg ccgagttcgt catcgatggc
    32161 attccctcct ccattccgct gctgcgccgc gtcctcgccg agcccgagtt ccgggcgaac
    32221 cgctacgaca cgagctggct cgagcgcttt ctcgcgaggc cgtcggcctg agccgtcacg
    32281 gccggtgctc cggacggcgc tgctttcgag gtcgccatgc agctgctcac ccctgagctg
    32341 gtcctcagcg cctattccat cggcctcttc ccgatggcca acgaccggga cgatccgacg
    32401 atccactgga tcgacccgct gcatcggggt gttctgccgc tcgaccgttt ccacgtgcca
    32461 cgcagcctca ggaagacgat ccggcgcggc cggttcgagc tttccgtcaa tcgcgacttt
    32521 ccggcggtga tcgccgcctg cgccgaggcg acgcccgacc gcccccgcac ctggctcaac
    32581 gacgacatca tcaggctcta tgtcgacctg caccggcgcg gccacgccca ttcgatcgag
    32641 acctgggaag ccggccgcct ggtcggcggg ctctatggcc tggcgctcgg cggcgccttc
    32701 tgcggcgaga gcatgttcag ccgggcgacg gatgcgagca aggtggcgct ggtcgagctc
    32761 gtggcgcgcc tgaggatcgg cggcttcgtg ctgctcgaca cacagttcgt caacgaccac
    32821 ctcgcccagt tcgggatcga ggagatcacg cgcagcgcct acaaggcccg gctcaagaag
    32881 gctctgacga tcgacgcctc gctgccggtc aatcccggct ccttcgccgc cgtgctggtg
    32941 ggtcgggcgt ccgccgccga cgatagctga acggtcagcc gtcgaacgag cggcgcagcc
    33001 tctcgagcaa ggccgtgcgc gccgcgtcgt ccggcagctc cttcagacga ccgctgagat
    33061 cgagcaccag gcgggcatag gcattgtgcc agtcgctcga tgccatcgcg gcttcctcgc
    33121 tgagccggcg ggcgtcacca tgccggatcg ccgattcctc ggcttcgaga tcgaagcggt
    33181 cgtcgagcgc cggcgcaatg acgtgttcgg gatcgcaccc gatctcggcc agcttcagac
    33241 gaaacgcgac catcgcatcc ggctcgccgt gggtcaggaa gatgcgcttg cgcaccggca
    33301 ggcgatccct cacccacgct accagctcgc cctggtcggc atgggcggag aagctctcga
    33361 tgctccggat ccgggcattg accttgatct cctcgccatg gatgcgaacc cggcgctcac
    33421 ccgactggat gatccggccg agcgatcccg gggcctgata gccgacgaag agcacggtcg
    33481 aatcgcgccg ccagagatgc tccttgaggt ggtggcggat ccgccccgcg tcgcacatgc
    33541 ccgaggccgc gacgatgatg gccccgccct tgaccttgtt gatcgccttg ctctcctcca
    33601 cgctctccgt gaaccggaac cacggcgcgt cgaagaagtc gcccttgcct cccacatcct
    33661 ccagatccgc ctgatgctgg gcgaagatct gggtgacctt gatggccagc gggctgtcga
    33721 tgaagatcgg cacttgcggg atctcgcccg cccggatgag cgccgacagc acctgcagca
    33781 cctcctgcgt gcgctcgacg gcgaaggccg gaatgagcag gttgccaccg gcggcaagag
    33841 cttcctcgac ctcgcggcac agcctggcgc ggcgctgctc cagggtcacc tcgtcccggt
    33901 cgcgattgcc gtaggtgctc tcgacaatga ggtagtcgac gtcctgcggc ccttcgggcg
    33961 tggcatggaa ggtcttctcg tccggcccga tgtcgcccga gaacagcagg cgcagtggcg
    34021 gctcgccggc ggcatcgacc tcgatctcga tcgacgccga gccgaggatg tggccggcgt
    34081 tccagtagcg cgctcggacc cccggcaacg gctcgagcca ttcccccaga tcgcaggtgc
    34141 ggacctgctc cagcgtgcgg atcgcgtcgg cctgcgtgta gatcggctcg acctgcggcc
    34201 ggccgcgctg ccgattgcgg cggttcagcc gctcgacctc gctttcctgg atgtagccgc
    34261 tgtccggcag catgaattcg agaagatcgg cggtcggccc ggtggcgacg atggaaccgg
    34321 cgaatccctt gcggaccagc ttcggaatga gcccgctgtg atcgatatgg gcatgggtca
    34381 ggagcagcgc gtcaattgag gcgggctcga aggggaagtc gttgtagttg agctcgcgaa
    34441 cggtcttggt cccctggaac agtccgcagt cgaccagcag gcgccccttc ggtgtctcca
    34501 ggaggaagca cgagccggtg acggtggagg ccgcaccatg gaagccgaga ctgatgctca
    34561 agcgatgctc ccggatagct gagacgcacc gtccgacgac tcggcgatga gcccgtaatg
    34621 ctcccggata tgacgccagg cgtcctcggc agtattgacg aagcggaaga gctcgaggtc
    34681 gctggcactc acgacctcct cctcgagcag cgcgtcgaaa tcgacgatcc gctcccagta
    34741 ctcacggtcg aagaggagaa cgggaatctt ccggaccttc tgggtctgca tcaggcacag
    34801 cgcttcgaac agctcgtcca gcgtgccgaa acctcccgga aagaccacga gggcgcgagc
    34861 gcgcatcagg aaatgcatct tgcgcaaggc aaaatagtgg aactggaacg acagctccgg
    34921 cgtcacccag cgattgggtg actgctcgtg cggcaaggtg atgttcaggc cgatcgagac
    34981 accgccggcc tcggcggcgc cacgattggc tgcctccatg atgccgggtc cgccaccggt
    35041 tacgatcacg aactgccgga ccggaccatc ctgggcatgc tccgttacca ggcgaccaaa
    35101 gcgccgggcc tcgtcgtagt agcgcgagcg ggcaaccgcg cggcgacatc tttcaagctc
    35161 gtgctgcctc gcgggatcct tcgggtcgtc cccggccgcg gcttcggcgc tggccagtgc
    35221 tgcccgcgcc tcctccggat caccgatgcg ggcgctgccg tagatgacga cagtcgactc
    35281 gaccagcatc gcctgcagct cgagctcggg cttgagcagc tccagctgca ggcggaccgg
    35341 ccgcagctcc tcgcgcagca ggaaatcggt atcggcgaag gccagtctgt agctggccgc
    35401 ctggctttgc ggcgatggga cgacatcctg ggaatgctcg agatcctcgc gcgcactcgg
    35461 gcggttgagg tgatcgtcct tgatgtcgcg catgatggtc ccgcggaaag tggctcgcca
    35521 tcctgcccac ctcgccgcgc agcgcaagcc cctagcgcaa cagcacaaaa aaaacgcaaa
    35581 cggccgccga tgcagcgcac cggcggccgt tcaaggaacg atcgagaagt cagctcaggc
    35641 ggcgagcgtc ttcgcctcgt cgaaattggc ggtggcgcgc ttgacgaaga gatcgacaac
    35701 ctcggtctgc gccttcatgc ccagatccat ggtctccttc gcctcggcca tggccttctc
    35761 gaggacgacc ttggcgtcat ccatgtaggc ctgcggctgc ttcttgccgt cgaagccctt
    35821 catcatgctc tcggtgctgc tgaaggcatc cttcagcagc tcgctctgcc gcttcgccac
    35881 ggtctgcgcc agatcgaaca tgatcttctg ggcggcgacc atcgtgtcga gattggcctt
    35941 gtgcagcgcc atcacggcgt cgacgtcgaa tttcggcatc atgggggtgg cgttgagagc
    36001 ggacttggtg gacatcgttc tctccctcga gattactgcg gtgcacaata gatagcatat
    36061 ccggcgacgc tcgcaagccg attcatgctg cactgcacaa attccgcctg cgacgcggga
    36121 gcgcacctgc gcaccgccgc caaagtctca agagggccag aaccggtcgc ccagcgccgc
    36181 ctcgagctcg ggctggccga gcggtgtcag atcctttgcc tgatcaccgg cgagccgttc
    36241 cagcacgggc gggtccaatt gctcgcgcag ctcgccgagc gcgcggggtg ccgccaccag
    36301 atgcagctcg tcgaaggccc gtcgtttcgc ctcggcgttc agatgccctg ccagcatcgc
    36361 caggaatcgt gccttcgcca ccgtcttggg tggcgtggtc ggctccatcg catggcgccc
    36421 ctggccggcg ctgtcgaagg tgcgcccggg acggtctgcc acgagatcga gacccttgcc
    36481 ggccgctgca tcgtccgccc acgatcgtag cagcacgaag cgacgcgacc ggcgatcgac
    36541 ggcaaagaga cgcccccggg cggcgtcggc aacgagcagc cagatggtct tgcgttcgag
    36601 tggcatgatg tctcccctga tgctgcgacg caaaatgccg tcgagcgcca ccggcttccg
    36661 gtcgcgctgc tcgaaaaagg aacacgacgg agcgtcgcaa ggtttcgggt agcccgtcta
    36721 ccgggatcga tgccagactg accgcaggca gggcagcagc ggaaacgggt gggaccatgt
    36781 tggcgagaat ggcgaagtcg gccgcagctc ttcttgcgac attcacggta ggtcaagcga
    36841 aagcggcgct ggcccctctc gatcccgccg ccgtcggcga ttggcactat gcgccgagag
    36901 cctggcccgg cctgccgctg agggccatgg acgaaaccca gcgccggctg ctctggcggc
    36961 tggtcgacct gacgctgagc gagagcggcg ccgcccgcgc ccgggccatc ctcgtgctcg
    37021 aggccattct cggcgagctc gagggccgtc cgaccttccg cgatcccgag aactaccatt
    37081 ttcgctgctt cggccggccg tcagccgaag cgctctgggg ctggcgcttc gaggggcatc
    37141 atctgtcgct caccgcgatg ctcgaccccg agcggggggt gctgccgacg ccgatcttca
    37201 tgggcgccaa tcgagcgacc gtgccgccgc cccatccccg ggcggggctg cggaccctgc
    37261 cggaggaagc agatcagggc ttcgcgctgc tgcacggcct ggatgacgcc aggcgcgccc
    37321 gcgcgatcct cgccgaacgc tcgcccggcg acgtgatcac cggccccggt cgcgagctga
    37381 gcctgcgggc tctcgaaggc gtgccgctgg ccgacctcct cgactcctcc cgagcggcgg
    37441 cgctcgccct cgtcgagctc tatgtcgggc gcctgacacc cgccctggcc accaccgaga
    37501 tggccgcgat ccgcgaggcc ggcacggacc gcattcattt cgcctgggcc ggctcaacga
    37561 cgccagggcg tgggcactac taccggctgc agggcccgag cttcgtcatc gagtacgaga
    37621 acagccagaa cgacgccaac cactgccact cggtctggca caacccgctg tcgcagttcg
    37681 gccgcgacct gctgcgcggg cacttccacc acagtcactg aggactgccg ggcagaaccg
    37741 gcagtccccg accgagcatg catgctcccg gtcggggacg ggctcgctcg aacgaaccac
    37801 cttcttggca agggccccgg atcgattgcg acgacctatt cgacgtcgct gccgggcagg
    37861 ccgccgagat agccgatcac tgccacgaac ggatcgatga gcgtttcgag ctcggccttg
    37921 agcgccgcgt ccttgatctc gaggtcgccg acatcgtcac cctgatgctg aaagctggtc
    37981 aggacatagg cgaagccgtt ccagccttcg accacgttga ggccagtcat ctcggcacct
    38041 gccgggccag acgcgacacg cgccagcttg ccggtgtcga cgttgaacgc ccacatgaag
    38101 ttgttggtgt gccggccgct gtcctcgtcg acgaacagcg tgcgcatccg gggcatgaag
    38161 gcgatgttgt cgggctcggc gatgtgatcg ggatcggccg tgttgcccat atcgtcactc
    38221 tgcgccaagt cgactcccat gagccccggc actcccgcca tggtgaccgc ggcccaatcg
    38281 ctggcgatcg gatcgccgtt gctgtccgcc acgccgctcg cggtctcgag aacatagaca
    38341 ccgccggcgc cgagctctgg cagcttgatg tgatcggccg gatcctccgg attggcgagc
    38401 atcccctcct ggatccgcga catggcgatg tagaccttgt ggtcgcgcgg atcgacggcg
    38461 acaccttcca tcttgttgaa ttccgtcgtg gccccgagcg acgccgcgta gcggcggctc
    38521 tcgaggaacg ccgcagcccg ctccttgcca gggttgagct tgacgtgctc gatattcttg
    38581 ctgccggcct tgatcgccgt gaagccgtcc ttcggctcgt cggagatggc gaagatgctg
    38641 ctctcggtcg aggagaattg ctcctcctcg atcatccggg caacctcgta gtcggcggcg
    38701 tggcccagct tgatccaggt gagattcgca ctgccgccgt tctggtccga ctgctggtcc
    38761 cagcgcccgg catagagcgt gccgacaccg agattgcccg cccgatccgc gacgtacatg
    38821 aaaaggccgg tatagcgccc gtcatcaccg taatagacgg tcttcccgtc cggcatgacg
    38881 cgggcgagct cccacgaggc gcgtcccatg ctgtagtgct tcttaaccga gctttgaccg
    38941 tcgcggccta ctgaaacctc gacgggataa ccgtagccgt aggggttggc cttgcgcgtg
    39001 ttgccgaaat agagttcggt caggccggcg agcgacgcgt cgaccttgcc tcgatcgtag
    39061 aggtcataat cctcctcgcc ggcgagatgc gtgccccatg gcgtttccga ggcagcgcag
    39121 ggaatccaca agccgccgac gcccgagaag tcgacattgg cctgatcgat taccgagaga
    39181 cggcctgtct gacgctcctg cgccaccagg ttgcggttca tgatcatcgg catccggggg
    39241 taccagccct cggtcttgga cgcctccttg ccgtcgctga gcagccagtc ggcttcccat
    39301 tgcacgagat ggtaggcagt cggatcaccg ttgggtgcgc tcggctgtcc cggcacccgc
    39361 gtgagcgtcg agccgtcggg attctcgctg atcacgggct ggccgtacgg gtcgatgagc
    39421 ccttggccga aggcgtcgta gagtcgccct gcctcgtagg gattgctgcc gattcggtcg
    39481 aggttccgat agagaacctc gtagcgcaac ggaaagacct tcttcgaccc gtcgtcatag
    39541 gtgacctcgg cgcgggccga gctgtaaatc cgtgccatgt cggcggctgt gctcggcgcc
    39601 tccatgccga cgaactcgac gctggcgatg ctcgcggcgc aaagtgtcgc cggcgcgatc
    39661 aggagcgccg tcaacgacac gccggcgaga gctgttgtct tgaagctcat ctcgatcctc
    39721 cccgttgccg cttggaccgg ttgcggtcaa cctcgacgtt atcatgacgt cgctcgcttg
    39781 caaacgtccg acgaggttgg atgcaaccca tcggctgccg caatcagggc agcgatctgc
    39841 gggtgaacga atcagtggac cgttggtcaa gaaggtcgag cgggccgctc gtccgagcct
    39901 ccgcgttcgg gcctgactgc attgcgacag ccagcctgac gctctcggct cggacgaacg
    39961 accgtcacag ctacgacagc tacgcagcct tggactgggc gccctggatc tggcgccaga
    40021 gcttctccgg cgtagccggc atgtcgacat tccggatgcc cagcggctgg agcgcatcga
    40081 cgatggcgtt gatgaccgcc gccggcgacc cgaccgagcc ggcctcgccg caacccttca
    40141 cgcccatggc gttgttggcg cagcgcactt cgatggtcga gaagtcgaag gacggcaggt
    40201 cgtcggcgcg cggcatgcag tagtccatga aggagccggt catgagctgc ccgctctcgt
    40261 cgtacaccgt gtgctccagg agcgcctggc cgatgccctg cacgatcccg ccgtgaacct
    40321 ggccctcgac cagcatcggg ttgaggacga caccgaaatc gtcgacgacg ttgtagtcga
    40381 cgatggtgac gatgccggtg tccgggtcga tctccacctc ggcgatgtgg cagccattgg
    40441 ggaaggtcca ggcatcgagc ttgatcgtcg tctcggcgtc gaggccgccc tcgatgccct
    40501 cggccggtga ggccatggtg cgcgccttct gggccagcga catgatgtcg atggtccggt
    40561 cggtgccggc gaccgagaac gtgccttcct ggaactggat gtcgg
//
LOCUS       DAOTFI010000006        40555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1040_length_40555_cov_6.676222, whole genome shotgun sequence.
ACCESSION   DAOTFI010000006 DAOTFI010000000
VERSION     DAOTFI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 40555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40555
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1040_length_40555_cov_6.676222"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..957)
                     /locus_tag="P5558_01060"
     CDS             complement(join(17..622,625..957))
                     /locus_tag="P5558_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611132.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HRY22929.1"
                     /translation="MPRPYSNDLRRKVVDTVRSGVSRRQAAGLFKVSVSFVIKLLQRV
                     QATGDVSPAQFGGYKVSPLVAHEADLRCWVAERSEITIAELQVLLSDRGTRTSPAALA
                     RFLAKLGLTKKKTARAAEQAREDVAAARQAWVGWQRGLNPASLVFVDETWTSTNMAPR
                     YGRGPKGQRVDGIAPHGHWKTTTFIAALRHDRITAPCVFDGPINGAAFRAWTEQALAP
                     TLEPGDIVILDNLGSHKVAGVKQAIEARGAELVYLPPYSPDLNPIEQVFAKLKHLLRK
                     ASARTVDALHDAIGKLLHEFSTQECRNYITNSGYPK"
     gene            complement(1031..1804)
                     /locus_tag="P5558_01065"
     CDS             complement(1031..1804)
                     /locus_tag="P5558_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY22930.1"
                     /translation="MEREVLPYIGDGDLASIRKRDIIEIIDRVADRGATVRANRVLAW
                     MRRFLNWCAARDLIDANPAQFVEKPSRETRRERVLDDGEIDLIWRALDASPQPFRDGL
                     RLLILTAAMRDEIFGARWSELDEAGRCLKLPAERSKSGTARIIPLSPPALAIVEALPR
                     FGANADRWLLTRCGKKPFSNFGHNKTALDGAAPLPDWRLHDLRRTAATGMQRLGFRLE
                     VIEAALGHVGGSRAGIVGVYQRHAFHDETREALDRIPKR"
     gene            complement(1813..2220)
                     /locus_tag="P5558_01070"
     CDS             complement(1813..2220)
                     /locus_tag="P5558_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HRY22931.1"
                     /translation="MLTKRLADTAEPFLGEDGRSRETVLWDASVAGFGLRVSPVGAKA
                     FILKYRAGRGRDATLRKVTIGRYGSPHTVETARREARRLLGIVAAGGDPAGDKAEKRR
                     DQRSGKATRGSVARACQEWLRRATRPATAPPQR"
     gene            complement(2468..3697)
                     /locus_tag="P5558_01075"
     CDS             complement(2468..3697)
                     /locus_tag="P5558_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY22932.1"
                     /translation="MIRFSNAPSGRHFLQIPGPTNVPDRVLRAIDNPTIDHRGPEFGA
                     LGRKVVSDLKTVFKTEQDVVIYPASGTGAWEAALVNTLSPGDRVLMFETGHFASLWRE
                     LAERLGLEVELLAGDWRHGVDPALVEARLAEDRRHAIRAVMVVHNETSTGVTSRVAAV
                     RQAIDAAGHPALLMVDTISSLASIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNAISAR
                     ALASQKTARLPRSYWDWAPMLAANRTGYFPYTPATNLLYGLHEALAMLLEEGLENVFA
                     RHDRHAEATRRAVLVWGLEILCADPAEYSSALTAIVMPAGHDADAFRKVTLQAFDLSL
                     GAGLSKLAGKVFRIGHLGSFNDLMLMGTLCGVEMGLGLAGVPHEKGGAQAAMACLAAP
                     ARLPKVERARARSKAPA"
     gene            3818..6892
                     /locus_tag="P5558_01080"
     CDS             3818..6892
                     /locus_tag="P5558_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRY22933.1"
                     /translation="MSTSAADRLPATPSSDVSERLARLLRSQTEGEVLFDAPSRGRYA
                     TDASIYQIMPVGVLVPKSERDIRAAIDIARDLKVPVLPRGGGTSQCGQTVGAALVIDN
                     SKHLRGIRELDVEARSVVVEPGVVLDHLNAELKRHGLWFPVDVSTSAQATLGGMAGNN
                     SCGSRSIAYGNMVHNVEGITAWLSDGELVELGPVAGLGGRGAAIAGFVERLAAQHRAE
                     IEERWPRVLRRVGGYNLDIFHNRSERPYTADSSVNLAHLLVGSEGTLACTRSLTLSLS
                     ELPRGKVLGVVNFPTFRAAMSAAQHIVKLGPVAVELVDRTMIDLALANPAFRPVMETA
                     LIGEPAAILLVEFAGEDKTALVPQLDRLDELMGDLSLPGSVVKMADDTAQKDLWEVRK
                     AGLNIMMSIKGDGKPVSFVEDCAVPLEHLADYTDALSEVFARHGTRGTYYAHASVGAL
                     HVRPILDMREAGPGGGAARMRAIAEEAAELVRKCKGSFSGEHGDGLSRGEWIAWQFGP
                     ALVEAFRAIKQELDPIGLFNPGKIIDPPRMDDRALFRFAPPDSLRPYRVVPLETKLDW
                     SAWDVRNDPVTEATTPPGTGGDPAGGFAKAVEMCNNNGHCRKLDAGTMCPSYRVTRDE
                     QHVTRGRANTLRLALSGQLGPDAFTSEATYETMDLCVGCKGCRRECPTGVDVARMKIE
                     FLAHYKARHGYTLKDRLIAHLPAYARAASRVPWLMNLRDRLPGAARASEILLGLSARR
                     RLPEWRSSTFWRATDPSLFATRDATLAAAAEGGKAAVLFVDTFNGMFESENAMAAARV
                     LRAAGYTLHTVEKAGGHHCCGRTYLAAGMVEQAKARAGALIEALLPLAEAGVAIVGLE
                     PSCLLTLRDEALVMGLGPRAEIVAGQALLFEEFVAREARAGRFTLELKAAEAPILLHG
                     HCHQKAFGAVAPILEVLRLIPETKVELIESSCCGMAGSFGYEAGHYDLSMQMAEAGLL
                     PALRRQPDAIVVADGTSCRHQIADGAGRQALHVVRLLERLLPATTKGGSASA"
     gene            complement(6935..7621)
                     /locus_tag="P5558_01085"
     CDS             complement(6935..7621)
                     /locus_tag="P5558_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY22934.1"
                     /translation="MSQHVIETPGQPVAPVRGSSATFPVRRIYCIGRNYAAHAVEMGH
                     DPDREPPFFFQKNPDNIDFSGEFPYPPMSTDVHHEIELVIALGRGGAYITIEDALDHV
                     WGYGVGLDMTRRDLQGEAKKLGRPWEIGKAFERSAPMTPLIPASEIGHPTQGAITLKV
                     NGQLRQAGDLNQMIWKTQEMIAYLSAHFSLAAGDLIMSGTPSGVGPVQRGDVLVGHIE
                     GVDTLNVRVV"
     gene            complement(7675..8733)
                     /locus_tag="P5558_01090"
     CDS             complement(7675..8733)
                     /locus_tag="P5558_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRY22935.1"
                     /translation="MSEEQAPTVAADRLERFVADALRHYDVPEEDANIVAGLMVEADL
                     LGHETHGVFRLRQYLARLRDGGCNPKARVAVVAERPATAVIDGDDGLGHLAMRAACRL
                     AMEKATACGIGWVGVRSGNHAGPAALYVRPQAQAGMIGMAAAVGSANHVPPFGGTDLL
                     LGTNPIAIAAPTGGADPFVLDMATTVAAMGKIKTLAQRGEAMPEGWMVGRDGKPLTDS
                     SRREEGFMLPIGGPKGFGLSVAIGLLAGVLNQAAFGSDVIDFTSESQRSSNTGQFVAA
                     IDIAAFGPLDTFTASVDKAFSEMRASEPLPGHARVRLPGEGKAALMAERRAKGIALHA
                     SLRKDLDDIAREIGVAPL"
     gene            complement(8726..9583)
                     /locus_tag="P5558_01095"
     CDS             complement(8726..9583)
                     /locus_tag="P5558_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY22936.1"
                     /translation="MSTQQPVVAPTEGDTVWTEQISFIDRVPRSLSMLFVFAVFVILW
                     QLATVSGLVSPIILPTPGETLQDLIFVGHNLITGGYMLPALWITIKEVIYGFALAIAI
                     GFSLGVLVGETGFGERAIMPYLVAIDTMPKVAFAPLFIAWLGFGIASKVALAAFIATF
                     PIVVGTAAGLHAADENARMLFKTMGASRLQTLVKMKLPHALPQIFTGLKIGAVGVMAG
                     AITGEFLGGGKGFGELIRVAASQLDTPRVFSLILYLSLLGLALFVLVSFAQRRIVFWR
                     KERVLADHE"
     gene            complement(9580..10479)
                     /locus_tag="P5558_01100"
     CDS             complement(9580..10479)
                     /locus_tag="P5558_01100"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY22937.1"
                     /translation="MPAASWRSRCLISPRRTPTSSSPSGTRVSDLTGSVALEMRPPLT
                     GRPVYELAHVGKTYARNQVTALQDVSLVLNKGSFSAVIGSSGCGKSTLLKIMAGLIPP
                     STGRVVLQGEPVIGARRDIGMMFQQATLFPWRTALENVVLPIEIRDGRAAAMAARERA
                     RELFEVVGLKGFEDVYPGELSGGMAQRTSICRMLITEPAVLLLDEPFSALDELSRDFM
                     NMELQRICTERDATAFLVTHSIPEAVILSDVVYVMKPRPGRIAEAVTIDLPRPRTLDM
                     ITTPKFGGLVDRIRGLLDREAFL"
     gene            complement(10391..11362)
                     /locus_tag="P5558_01105"
     CDS             complement(10391..11362)
                     /locus_tag="P5558_01105"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY22938.1"
                     /translation="MSAAAGVGTAQDLRQITFVQPSPSAINSFPVFVAIGEGFFEEEG
                     LDVRVEAINGSAAVLQALSAGQAEFGRPGPAPVLAARSRGVDAVFIYNVAARSNFGIV
                     VPVDSEIQSIEELEGKVIGTGTADGAEVGFARSVMLSADMKEGEDYEFLTVGDGGPAT
                     VAFQSGAMAAYSASTADAAILNQRGMPVRDITPAEFARFFGNGLATMGDTIRNDRELV
                     EKFGRAFARGHAFALDDANREKVLAHLKAGNPQEGEDAEFQSALFDAVRSKTIPVDMS
                     NGLGYMPPEVWEEWQENLIAGGELAEPLPDLTEAYTNEFVAEWNQGL"
     gene            complement(11473..12390)
                     /locus_tag="P5558_01110"
     CDS             complement(11473..12390)
                     /locus_tag="P5558_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY22939.1"
                     /translation="MRLVTLLIDGAPRAGVVLANGNVLDLAEAAREAPGAAGEAVRTA
                     SLQSIIEAGPKALDLVRTLADRSDRSAQRRPEDHVTMAPLPSPAKNVFCVGRNYAEHI
                     AEGDRAQNQKVGVTEHPVFFTKPRTAVIGPGAAIPIFPHVSTSIDYEVELALVIGRRG
                     RDIAREDAFDHILGYTIINDVSARDIQRRHGGQYFKGKGLDGSCPMGPCIVTADEIGD
                     PGNLAISLTVNGETRQSSTTARMIFDIPTLIASLSAGMTLEPGDILATGTPSGVGYAM
                     DPPRFLTDGDVVVCTIENIGSLENYCRVQ"
     gene            12574..13305
                     /locus_tag="P5558_01115"
     CDS             12574..13305
                     /locus_tag="P5558_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY22940.1"
                     /translation="MSRSDRHALAERVRTFIGEHGYDHNQRLPPERELCALLDVSRSQ
                     LRNALAVLEENGLIWRHVGRGTFIGARPVLNLDDITFLRELASPEQILESRTAIEPAL
                     AQLAAMHGTKADLEEITACAARCRNAADWRSYEAWDNNLHDAIAKATHNKLLIYLFET
                     LNVVRRTVVWGQTRTTRGPKADHVSFTEHDAIIAAIKVRDADQAASAMLAHLRSVRER
                     VLPMLILRDAGDRRTLFEQQAAPAR"
     gene            13664..14476
                     /locus_tag="P5558_01120"
     CDS             13664..14476
                     /locus_tag="P5558_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY22941.1"
                     /translation="MRMTIAAAAIGLALSAPAAFADKLDDIIASGVLRCAVVLDFPPM
                     GSRDANNEPIGFDVDTCNDLAAALGVEPEYVETPFPDRIPALVSGRADVGVASTSDTL
                     ERAKTIGFSIPYFAFEMVVLTKDGLGIDSYESLKGHKMGSVSGTFEAIRLENDVDAWA
                     DANGSFTPYQTQADVFLALSQGQIDATVVTSTVANAAAKSGDFEGFVVGGKAPYDIDY
                     TALIALREEYGLLNYLDLFVNQQVRTGRYAELYAKWIGSDEPPDLTVPGVYR"
     gene            14571..15233
                     /locus_tag="P5558_01125"
     CDS             14571..15233
                     /locus_tag="P5558_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY22942.1"
                     /translation="MNYTFHWRPAFRALPEMLAGSLVTLQIAVLSMVIGIAIAVLLAV
                     MTGSKSRWLRGIAGTWIEVARNTPALFQIYIAYFGLGQIGIHLDSYLALLAGITFNNA
                     GYLAETFRGGLRAVPQTQVRAARSLGFGPIAAFRLIVLPQLFRAVFHPMTNQMVWAIL
                     MTSLGVVVGVNTDLTGVTQELNVRTFRTFEYFAIAAVLYYLIAKVVTLGARLIAWRLF
                     RY"
     gene            15235..15891
                     /locus_tag="P5558_01130"
     CDS             15235..15891
                     /locus_tag="P5558_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY22943.1"
                     /translation="MLDTALSLNDLRFLMLGAGWTLLITAISVTAGTLLGVLFGVARA
                     QTPVWVSLPIGFVLDIFRSVPLLIQLVLFNSFIPILGLRATPFSVSCLVLALYTSAYC
                     SEIVRGGVAAVPQTTRRAARSLGMTWGQDMVEIVFPLALRVMFPSWIGLTLGVMKDTA
                     LVAWIGVIELLKSSQILITRMPSEPLFILGIAGLIYFLISFPISRLGARLERKWREND
                     "
     gene            15884..16606
                     /locus_tag="P5558_01135"
     CDS             15884..16606
                     /locus_tag="P5558_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRY22944.1"
                     /translation="MIEIENVRKSFGQLEVLKGVSLAVDRGEVVSVIGGSGSGKSTLL
                     MCINGLEPIDSGRILVDGVEVHARSTDLNKLRRKIGIVFQQWNAFPHLTVLENTTLAL
                     RKVLGKSRAEADAIAIKQLEHVGLQDKLKVFPGKLSGGQQQRMAIARALAMSPSYMLF
                     DEVTSALDPQLVGEVLDTMRLLADEGMTMIVVTHEMGFAREVSNRVAFFKDGLIHEIG
                     TPKQIFEQPQRPETAEFLGSVL"
     gene            16635..17738
                     /locus_tag="P5558_01140"
     CDS             16635..17738
                     /locus_tag="P5558_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY22945.1"
                     /translation="MKIAAIRVYQIDLPLKEGRYSWSNGNFVEVFDSTVVEVETDSGL
                     KGYGECCPLGSAYLPAYALGVRSGLRELAPQVIGMDPRDLRVLNRHMDAVLRGHPYVK
                     SAIDVACWDILGQAAGMPVYKLLGGAAQADVRLYRAISQEAPEEMARKIAGYRAEGYT
                     KFQLKVGGNADDDIERIHACAGLMQPGDILVADANTGWTMHEAARVVQAVRDIDVYIE
                     QPCPTYRECLVTRRRTALPFVLDEVIGDVDTLMQGIADGAMDVINLKISKVGGLTKAL
                     LMRDICTTAGIPMTIEDTWGGDIVTASIAHLALSTPEEFAFSATDFNSYGTVEFASGA
                     PRRVDGRMRPAEAPGLGIEPMMDVLGKPLLTVP"
     gene            17749..18810
                     /locus_tag="P5558_01145"
     CDS             17749..18810
                     /locus_tag="P5558_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRY22946.1"
                     /translation="MRSSKVLHVVSCHAEGEVGDVIVGGVPPPPGATIWEQARWIHQD
                     QRLRNFVLNEPRGGVFRHVNLLVPPKDPRAVTGWIVMEPVHTPPMSGSNSICVATVLL
                     DTGIVPMEEPETRFVIEAPGGLVEVVANCRNGKAESVRVVNHPSFSARLDAMIEVEGL
                     GSLKVDTAYGGDSFVIVDAAAVGLEIRPDEAFDISRLGMRITRAANEQLGFTHPLNPD
                     WRHISFCQFVNPVREIDGVPTGRNTVAIEPGKLDRSPCGTGCSARMAVLHARGLLGQG
                     MRFRGISIIDSVFDCRVERETEVGGLAAIVPSIQGRAWITGTHQHMLDPADPWPEGYR
                     LSDTWPIRAVPGSQQEGTA"
     gene            18811..19749
                     /locus_tag="P5558_01150"
     CDS             18811..19749
                     /locus_tag="P5558_01150"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY22947.1"
                     /translation="MDISVFKGCMPALMTPCTADRRPDFDALVDCGRRLIDAGMRAVV
                     YCGSMGDWPLLTDAERQEGVARLVAAGVPVVVGTGAQSPKAAVAHAAHAQKVGAKGLM
                     MIPRVLSRGSSLAAQRAHFSAILAAAPDLPAVIYNSPYYGFATRADLFGELRRSHKNL
                     VGFKEFGGAEAMTYAAEHITGAEDGLLLMVGVDTCVFHGYVRCGAVGGITGIGNALPA
                     EVLHLIRLCEQAREGDVDARRKAQELESALAVLSWFDEGADLVLYYKYLMTLEGDPAY
                     EHNFVETDELSASQKHFARTQLAQFKAWYAHWSRPR"
     gene            complement(19768..20742)
                     /locus_tag="P5558_01155"
     CDS             complement(19768..20742)
                     /locus_tag="P5558_01155"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY22948.1"
                     /translation="MCTSQIVDEVTRRRIAAHGASFRQASSSPFGARDEIGMLNLMDA
                     ASAQAIMARADAGRMFDLSVDHFMGMPGWFGAGDPPYQIWMTHTPRGEEADDVMGVGR
                     TQNELVSYSGDVVQMYTHCGTHIDTLNHFGYHGEIFNHFHADEHLGSRHWRVCGAEKH
                     PPMLARGVLLDIAGMHGVDMLPDSHPVGPVDLAACLRHQAVELRPGDVVMIRTGRMRA
                     WPNQDAFTLNAPGLNRAGAEFLAKAGAITIGSDTLALEQTPSADPENWMVVHSYLLAE
                     AGIPIMEVVNLEEIGANKLWEFAFFGACIRLRGATGAPMRPVAMPLRD"
     gene            complement(20780..21706)
                     /locus_tag="P5558_01160"
     CDS             complement(20780..21706)
                     /locus_tag="P5558_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRY22949.1"
                     /translation="MMEGEAMVDWSAGAAYIDGRFMPLAEARIPITEWGYRRSDVTYD
                     VTGVFFGAFFRLDDHIARFRRSMDRMRMQPGESDAEIREVLMELVRRTGLREAYVAMD
                     CLRSRPPRGMPMLPVNARAYLVCFAVPWVWLFSEEQQARGVHAMIAKTPRIPAASVDP
                     TVKNFHWGDLTAALFEAQDAGFDTAVLLDGAGNVTEGPGYNVFAVIDGTVVSPPEGAL
                     EGITRQSVFELCEGLQIPCEVRPLSADELEEADEIFFSTTAGGVMPVSRLGGRILGND
                     RPGPISANLRETFWERRRQGWHATPVDYPSTT"
     gene            complement(21703..23538)
                     /locus_tag="P5558_01165"
     CDS             complement(21703..23538)
                     /locus_tag="P5558_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY22950.1"
                     /translation="MNDANEGQATPAEMEGAGWRRDGVRMAMISNRIESIARKMQNTL
                     FRTARSGVLNTAHDFSCVILTADCRLLGSAESLPIHTLIGPDLMCEAVKRHHPTLSAG
                     DCFLHNSPYEGDSHPADHCLIVPVVDEGATLRFFVLAKAHQADCGNSRPTTYMGHATD
                     VYEEGALIFSAARIQTGYRDNDDLIRMCRARIRVPEQWWGDYLATLGAVRIGERELLE
                     LGRDVGWPTLASYTENWFDYCERKMIAAIRRLPSGRIRVQSAHDPFPGVPDGIPVSID
                     VEIDAENAMISVDLRDNIDCQPCGLNLSEGCARTAAMVGIYNGIIDHTVPANAGSFRR
                     IDIKLRENCVVGIPRHPHSCSVATTNLADRVASPVQRAIAEIAPGFGMAETGPIQPPG
                     MGVISGRDPRTGEPFVNQIFLGITGGAGTPQSDGFLTIIHVGNAGLCRHDSIEVDELH
                     HPIFVKDRRLLTDSEGPGEFRGAPSAYVEFGPIEGCTMQVLYTADGTVNPAQGARGGW
                     PGCRAEAFKRELDGSLTTLPACHGVTLVAGEQVVSHSAGGGGYGAPASRDPERVLHDV
                     LEGWISTERARRVYGVVIEAEGTGMVIDRAATRALRADAGDGKDT"
     gene            complement(23557..25362)
                     /locus_tag="P5558_01170"
     CDS             complement(23557..25362)
                     /locus_tag="P5558_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY22951.1"
                     /translation="MTTAATGTPHDAIRMALFSSRIESIARKMQNTLFRTARSGILNT
                     AHDFSCCILTADCRLLGSAESLPIHTLIGPDLMCEAVKRHHPVLRRGDCFLHNSPYEG
                     NSHAADHCLIVPVMDDDEVLRFFVLAKAHQADCGNARPSTYLADVVDVYEEGALIFSA
                     TRIQSDFKDNEDIIRMCRARIRVPEQWWGDYLAALGSVRIGERGLLELGREVGFAALD
                     DYAEAWFDYSERKMVEAIRRLPSGKATVRTAHDPFPGLPNGIPIKVEVEVDAEAGRIT
                     VDLRDNPDCQPSGLNLTEGTSVSGAMVGIYNGIMDHSVPANAGSFRRLDIKVRENCCV
                     GIPRHPHSCSVATTNLADRVTNPVQRAIAEIARGFGMAETGPIMPPGIAVISGRDPRH
                     GDEPFVNQLHLAISGGAGTPTSDGFLSIIHAGNAGLSRIDCVEVDELHHPIIVTTRRL
                     VPDTEGAGEHRGALAIHGEYGPIEGCNLRVLYTMDGSINAAAGAVGGLSGAPSRAFRR
                     NRDGSLTELPACHGLTLAPGERVVSHSAAGGGYGSPLDRDPELVLRDVAEGWVSAERA
                     STVYGVVLTGEASADRLAIDLAATDQRRADLRTGS"
     gene            complement(25376..27436)
                     /locus_tag="P5558_01175"
     CDS             complement(25376..27436)
                     /locus_tag="P5558_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRY22952.1"
                     /translation="MRLACDTGGTFTDLVVERGDEIRQFKSSTVPADPVQGVLDVLGL
                     AAAAHGLDRAAFLAEATTFIHGTTRAINAILTGNTARTAFLTTKGHPDVLVIREGGRI
                     EPFNFTVPYPEPYVPRRLTFEVPERIDAAGRVVEPLDEATVLAIIERLKALEVEAVGV
                     CLLWSVVDGAHERRVGELLAEHLPGVSVTLSHVLNPTLREYRRASATVIDASLKPLMF
                     AYLNGLTDRLRQAGFNGRTLMVTSGGGIMDAEDVARAPIHSINSGPAMAPVAGRFYAQ
                     RDQQNAMAIVADTGGTSYDLSLVRDGRIPWTRETWLGQRFRGHMTGFPSVDVKSIGAG
                     GGSIAWVDSGGLLRVGPKSAGSTPGPVAYGKGGTEPTVTDCSLILGHIDADFFLGGAM
                     ALDRAAAVAALETNVAAKLGLSAEEAAAAVLALATEKMVGAIDEITVNQGIDPSSAVL
                     IGGGGAAGLNAVAVGRRLGCAGVLIPEAGAVLSAAGALMSELASDYARMQFTTSQNFA
                     YEAVDEVLAALEKRCEAFANGPGEGSLETLIEFSVEARYPHQIWEIEVPLAGRRVDGP
                     EALAALKSAFHDTHKAIFEISDPHSEVEFVTWRAKVRCRLREAATGRVPPPDSQSDLP
                     VSRRVYFAGSGWVEARVARFEAMPADEPVPGPAIVESSFTTVVVDPGTVAVRTTGGGL
                     RVTF"
     gene            27619..29220
                     /gene="groEL"
                     /locus_tag="P5558_01180"
     CDS             27619..29220
                     /gene="groEL"
                     /locus_tag="P5558_01180"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRY22953.1"
                     /translation="MNAKVVRVGEAARQPAMRGIDLVADLVAGTMGPAGRAVLVGRNH
                     APPLLLRNGYAIARNLELGSRDEQAGVLLMRDLAWRTSDAVGDGTSTAILAARAFVRA
                     GRLAALSGIAPSELQAAIDAHSTAVLEALEAASCPAPEAAVLERVATQALGGDAALGA
                     LLAEAHLQAGSDGMVVIEEGRSAEDTLRFDAGLHFDQGWISPHLVDDARTRTIEIENP
                     LILVHAGPITALQPVVRVLEMIAESGRGLVIIADSVAEDALTTLIANKRGAGLKVAAI
                     KAPGAGAWRCSTLEDIAIATGAALIAEELGHSLAGLRPQFMGGAARVIVRRHDSTIIG
                     GKGDPAAVATRTMEIRDAITREKHLSFDREQLRKRLARLQAGIATLGIGGRTPTHLQL
                     RLEQARNASATLAAALSGGLVPGGSAALVHAERRAAAALPEGLLGSLLGRMFAAAAAA
                     PLREVVRNAGADADLALGRIAADPGTTFDVRSRAIVPVDTIMDPAPVAVAAFRGAVST
                     ASRLLGVAASLHEAGHASDRTTALD"
     gene            29395..30567
                     /locus_tag="P5558_01185"
     CDS             29395..30567
                     /locus_tag="P5558_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY22954.1"
                     /translation="MAQLAARLATKPVQKAATGAGEEDSSPAPETVGKPILEVIDAVH
                     VYGDVVALDGVTVSAAAGEFLTILGESGSGKTTLLRLISGLEKPYRIGALRLGGVDVS
                     TVPAYLRNCTTVFQHYALFPHMSVGENVEYGLKVRGVKPAERRTRAREALRLVRLEEK
                     HDRRIHQLSGGERQRVALARALAPRPAILLLDEPIGALDEKLRLDMQVELLEIHKRLG
                     MTFVYITHSQEEALTMSDRIILMRKGRIEQSGPPTDIFDRPVNRFVAAFMGVENLLEA
                     TLLTLDGDHATFRLGEATFSGRWSGTGSPEIGSDIVVGLRAERLHVADAAPESPAAAN
                     VLPCRFGAAIYKGKYLDRTANTAIGQLKVRIWDANPVTRDDAFIWWKREDCMVMSP"
     gene            30656..31840
                     /locus_tag="P5558_01190"
     CDS             30656..31840
                     /locus_tag="P5558_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY22955.1"
                     /translation="MSSFDISRRSFVDKALTLGGLAAAAGSVPLLGRQALAAGETVKA
                     YGTTTAQLRDWSIMENSIGIKVEFTPTNTDIGIFMRDIISNDLGATQDIFIFDGGTEN
                     LLGPQGYYAEIIEDQPELTLWERTPDAWKRADWLRFDGKQYGVPVIGNGDAFGYFPEV
                     IDANPDGMDEIPWTTFFEDERVKGRVAIDRSWLQSLPETANFLKHHGRVAIEDPADLT
                     PEEARPVVDFLIERKRAGHFRTIFTAFEEQIQLLSNREVDMINCWEPAVKEANEALGP
                     GTVIYAFTVEGYYKWGHSANVSSQAMERGNLDNIYKLLNYFLGGEYRAYQAKQRGYAG
                     PNMDLGVAYATEHGWPQEDIDLLKWTEEKVARKFEKPFWANTTPVNADVMESEWQRFL
                     NA"
     gene            31916..32836
                     /locus_tag="P5558_01195"
     CDS             31916..32836
                     /locus_tag="P5558_01195"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY22956.1"
                     /translation="MATADLTRKETALDRLAARLSPADLAVPTFLVVWSAVVILPLLI
                     LVVFSFFQVRSYQIVYEPTFATWQSVVETGRWTVALRTLRVALTMTVIELLLAFPFAL
                     WLAKGLRSKTIKAIIITFLTIPFFLDVSSRILVWRSILGTNGIINTVLTSTGLVDEPI
                     SWLLYSEFAVHFGMLGANFPTMVFPIFIILALIDDDYIHASGDLGASPVQTLLHVIIP
                     MALPGILAGIIFTFVPLMAAFVEPQLLGGGFVDLLGNSVDSALQQIRYPMAAALSTVV
                     VVLLGLCLGAFMLLTRNRLDLATIFSALHR"
     gene            32833..33699
                     /locus_tag="P5558_01200"
     CDS             32833..33699
                     /locus_tag="P5558_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY22957.1"
                     /translation="MSTSTLPVALVDESEIAPTIRLLRQASRWRVWPGVAMALGFFAI
                     AAIYIPLVWLAVMSFSARPLSGIPYPLTLANFSLLLGDERWVPPLWTSIWMSALVALC
                     CMIVATFVGRAITRMRRPGPVLLLTLLPLFVPGLTMGAALFIFLRILMGLTLGLWSIF
                     IAQLVWALPFALLLVLVVASRFDIRLLEAAEDLGASPWRRFWEIEMPLLRPGVLGAGV
                     FGFLLSFNELLRSLFVRGIETTMPIYNWAMAASQQSQVPIIFALATIILFVTLPVMGA
                     FFWFLFVKLDRD"
     gene            33782..35194
                     /locus_tag="P5558_01205"
     CDS             33782..35194
                     /locus_tag="P5558_01205"
                     /inference="COORDINATES: protein motif:HMM:NF004767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRY22958.1"
                     /translation="MLDERPNFSLEDMDRESLFHPLTSIAAHQKNGPLIMDRGQGAML
                     RDHGGNEIVDCSGGLWCVNVGYGRPELAEAAKQAMLDLNYWHLFGSSSHESAIRLADR
                     ILQLLHEQAGARHLSKVFFGTSGSDANDTNYKLVRYWATLRGTPEKKKIISRTGGYHG
                     LTTIASALTGIEAYHKAWNLPEVDVRYTSCPHHYRFAEDGESEAAFCDRLIAELEAMI
                     EREGPETIGGFIAEPIMGTGGVLIPPAGYFERVQAVLNRHDILFIADEVISGFCRTGE
                     WFATGLYDLKPDLLTLAKGVTSAYFPVSASVVSERVWKVLEAGSPKHGAVMHGFTYSG
                     HPVGCAVGLANLEILERESMAANAAAVGGHLLAGLKDRLGEHPFIGDIRGEGLMIGVE
                     FTADRARRRPFKAETVPHRLVAAKALEHGLMVRGLPFIEVISFSPPLCITKAECDQAV
                     DRFAAAVDEMTPELTRLAAG"
     gene            35195..35962
                     /locus_tag="P5558_01210"
     CDS             35195..35962
                     /locus_tag="P5558_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY22959.1"
                     /translation="MAAREPADGGSAGAASAPREGAPPREGHPPTEGPEARTLAQRVY
                     ETLEKRILDGEWLPESKVSLRTLAISLDTSMQPVREAVGRLIDAAALRSAPGKAIRVP
                     RLDRRTADEIWSLRFLLEGEAAARFAARKRPAEARQLYTYTKALRAMRFGADLQPTMR
                     RMMAWNLALATGSGSPILIDIIRRLRLRYAPFVAEALAEEAPFDLEFHQFTLHMQDEL
                     VMAIEGGDADAARHLRGADLRSFQRYLYARKSWRPIA"
     gene            36028..37065
                     /locus_tag="P5558_01215"
     CDS             36028..37065
                     /locus_tag="P5558_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRY22960.1"
                     /translation="MQVFFHEAQRQHDPQHFLVAGTLRPSPEQPARIDALLAAARNLG
                     LEVQSPGDAGMAPIAAVHSTDYLSFLQTIHERWSALSGAGPEVVPNIHPEGRSLVYPA
                     HPAGLAGYHQADTACPIGPHTWHSAYWSAQTAIAAADAVLRGAASAYALCRPPGHHAT
                     SDRAGGFCFLSNAAIAAERCLRAGRRPAVLDIDLHHGNGTQQVFYERQDVLTVSIHAD
                     PSRFYPFFWGHAGERGAGPGHGYNLNLPLALGTGDDGFLAALAEASERIRLFGADLLV
                     LAVGLDAFEGDPFAGLAVTTGGFGRIATACASLGLPTVLVQEGGYLSPELTRNLESFL
                     ASWLRPAPSGR"
     gene            37084..37833
                     /locus_tag="P5558_01220"
     CDS             37084..37833
                     /locus_tag="P5558_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRY22961.1"
                     /translation="MRLLLINGNTTQFVTDAAAREARLAAADGTEVVAVTGRSGARII
                     GTRFENALAQHEMIALAAAHGEGIDAVLIAVSYDTALPALRELLSVPVVGMTEAALLT
                     AHLAGGRIGMVSLGRRVQPLYRELAAAYGLDRRIAGWRTVESTKAFAPGDTAELDGLL
                     AGACNELVEQELAEVVVLLGAVMAGAPRRLQARVPVPLVEGIASGVGQAELLVRLGLP
                     KPGAGSYALPGRRELAGVEPAILALLRRQPG"
     gene            complement(37830..38549)
                     /gene="rpiA"
                     /locus_tag="P5558_01225"
     CDS             complement(37830..38549)
                     /gene="rpiA"
                     /locus_tag="P5558_01225"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRY22962.1"
                     /translation="MTPQDIAKRAVAAAAVERLVAPGMKLGLGSGSTSEWMVRALGER
                     VRAGLDVVGVATSTGTAALAREVGVRVIELDEAGELDLTLDGADGIDHRFRMIKGGGA
                     CLLWEKIVAMASRRMVALVDETKVVETLGAFPLPVEVVRFGWQATRRQIGQILAEHAH
                     VEPRITLRGGEGGPLLTDSGHFLLDCRLGAIPDPPALEAALNRVPGVVENGLFSTVAD
                     SMMVGYADGSTRMFGPYASAV"
     gene            38670..39929
                     /locus_tag="P5558_01230"
     CDS             38670..39929
                     /locus_tag="P5558_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRY22963.1"
                     /translation="MTQTTLRDDELLDLVQRQTLRYFWDFAHPVSGLARERSDVTPGC
                     GPDVVTTGGTGFGIMAIIAGVERGWLDRAAASNRLLEMARFLGHAERHHGIFAHWLDG
                     RTGRTVAFSELDDGADLVETSFLLMGLLTARQYFGGNGAAERELRERVDALWNEAEFD
                     WHTKGDEDMLYWHWSERHGWAMNHPIRGWNECLVTYVLAASSRRHAVRPEAYHRGWAG
                     GSRFANGRTYHGIRLPLGPDHGGPLFFAHYSFMGLDPRGLEDRYADYLEQNVAHTLIN
                     HRHCVANPNGHAGYGEACWGLTASDDPWGYEAHAPDRDRGVITPTAAIASMPYTPALS
                     LAALRHFMDYDDGRLWTDMGFVDAFCPAEAWRAGSHLAIDQGPIVVMIENHRSGLLWR
                     LFMSCPEIRDGLRRLGFTSPHLGATSA"
     gene            complement(39946..>40555)
                     /locus_tag="P5558_01235"
     CDS             complement(39946..>40555)
                     /locus_tag="P5558_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRY22964.1"
                     /translation="VDADGYFPSRVPPLRARLDPLAAGDEMRAQIERAMAMGIRPTHL
                     DTHMGAALVPELADITIGLADEYRLPLLFPREIESYLGVLNLGPVDPAFYTERHAALA
                     ARGAAFVDRFVMTPWVASALSDATYRQMIDALRPGVTFFSLHPNAPGDIEVIKPDLAH
                     CRTDEHRLGRDPGFLRHVEDSGVVPIGFAPLLERLRAATARA"
BASE COUNT         6317 a        13798 c        13851 g         6589 t
ORIGIN      
        1 ctagtgcgtt tcccgtttac ttgggatagc ccgagtttgt gatgtaattt cggcattcct
       61 gggtcgagaa ctcgtgcaga agcttgccga tggcgtcgtg cagggcatcg acggttcggg
      121 cgctggcctt gcgcaagaga tgcttgagct tggcgaagac ctgctcgatc ggattgaggt
      181 cggggctgta aggcggcagg tagacgagtt cggcaccacg cgcctcgatc gcctgtttga
      241 cgccggccac tttgtggctg ccgagattgt cgaggatgac gatgtcgcca ggctcgagcg
      301 tcggcgcgag cgcctgctcg gtccaggcgc ggaaggcggc gccgttgatc ggtccgtcga
      361 acacgcaggg tgcggtgatc cgatcgtgtc gaagcgcggc gatgaaggtg gtcgtcttcc
      421 aatggccgtg tggggcgatg ccatcgaccc gctggccttt cgggccacgc ccatagcgcg
      481 gcgccatgtt ggtggaggtc caggtttcgt cgacaaagac gaggcttgcc gggttcagcc
      541 cgcgctgcca gccgacccat gcctggcggg ccgcggcgac gtcttcgcgc gcttgctcgg
      601 cggctcttgc cgtctttttt ttcgcgtcag gcccagcttg gcgagaaacc tcgcaagggc
      661 cgccgggctc gtcctcgtgc cgcggtccga cagaagcacc tgcagctcgg cgatggtgat
      721 ctccgaacgc tcggcaaccc agcaccgcaa gtccgcctcg tgcgccacca gcggcgagac
      781 cttgtagccg ccgaactgcg ccggcgacac gtcaccggtc gcctgcaccc gctgcagcag
      841 cttgatgacg aagctgacgc tcaccttgaa cagcccggca gcctgccggc gcgatacgcc
      901 ggaccggacc gtatcgacaa ccttccggcg tagatcgttc gaatagggac gtggcatccg
      961 tggatggcct cctcgtccag ccaacagctt gaatcacatc tatcaagagt tgggaatccc
     1021 tgaaatgatt ctatctttta ggaattcggt ctagagcctc gcgggtctcg tcgtggaagg
     1081 catggcgctg gtagacgccg acgatgccgg ctcgcgatcc gccgacgtgg ccgagggcag
     1141 cctcgatcac ttcgagccga aagccgaggc gctgcatccc ggtggcagcc gtgcgccgca
     1201 gatcgtgcag ccgccagtcg ggcaggggag ccgccccgtc gagcgccgtc ttgttgtggc
     1261 cgaagttgga gaacggcttc ttgccgcacc tggtcagcag ccagcgatcg gcgttcgcgc
     1321 cgaaccgcgg cagtgcctcg acgatggcca atgcgggcgg cgacagggga atgattcgag
     1381 ccgttccgct cttgctgcgc tcggccggga gcttcaggca ccggccggcc tcgtccagct
     1441 cggaccagcg cgcgccgaag atctcatccc gcatcgcagc cgtcaggatt agcagccgaa
     1501 gcccgtcgcg aaagggctgg ggcgacgcat cgagcgcccg ccagatcaag tcgatctcgc
     1561 cgtcgtcgag cacccgttcg cggcgcgtct cgcggctggg cttctcgacg aattgagccg
     1621 gattggcgtc gatcaggtcg cgtgcggcgc accagttcag gaagcgccgc atccaggcga
     1681 gcacgcggtt cgcccgcact gtggcgccac ggtcggccac ccggtcgata atctcgatga
     1741 tgtcgcgctt gcggatgctg gcgaggtcgc cgtcgccgat gtacggcaac acctctcttt
     1801 ccatgatccg cgctacctct gtggcggtgc ggttgccggc ctggtcgcgc gccgcagcca
     1861 ttcctggcag gcacgtgcca cggagcccct ggtggccttg ccggaccgct ggtcgcgccg
     1921 cttctcggcc ttatcgcccg cgggatcgcc gccagccgcg acgataccga gcagccgccg
     1981 cgcctcccgc cgggccgtct cgaccgtgtg cggcgagccg tagcggccta tggtcacctt
     2041 gcgcagcgta gcatcacggc cgcggccggc tcgatatttg aggatgaacg ccttggcgcc
     2101 aaccggggag acgcgcaggc cgaagcccgc cacgctcgcg tcccagagca cggtttcgcg
     2161 ggatcggcca tcctcgccga ggaacggctc ggcggtgtcg gcaaggcgct tggtgagcac
     2221 cgaagtgacc ctctctgccg cagtccgcgg gtggaatcgg tggtaccttt cgcgcttggt
     2281 ggtaccccat gacagtatga aagaaaatag tcgagaattg cagaggaggg tcaagttgct
     2341 gagaaaatgc tgtatggtcg ccccgagaaa atagcagaat atgtgagaaa aatccgatag
     2401 acggactttt aatccgcggg ttccgggttc gagtccccgg ggggggcgca ccaactgatt
     2461 cattaactta cgcgggcgct ttcgaccggg cgcgcgcccg ctccaccttc ggcagccgcg
     2521 cgggcgccgc caggcaggcc atcgccgcct gtgcgccgcc cttctcgtgt ggcacgccgg
     2581 cgaggcccag gcccatctcg acgccgcaga gcgtgcccat cagcatcagg tcgttgaagc
     2641 tgccgagatg gccgatgcgg aacaccttgc cggccagctt cgacaggccg gcgccgagcg
     2701 agaggtcaaa ggcctgcagg gtcaccttgc ggaacgcgtc ggcatcgtgg ccggccggca
     2761 tgacgatcgc tgtgagggcg ctcgaatatt ccgccgggtc ggcgcagagg atctcgagcc
     2821 cccagactag tacggccctg cgggtcgcct ccgcatgacg gtcgtgccgt gcgaagacgt
     2881 tctcgagccc ttcctccagc agcatcgcca gggcctcgtg cagcccgtag agcaggttgg
     2941 tcgccggcgt gtaggggaaa tagccggtcc ggttggcggc gagcatcggc gcccagtccc
     3001 agtagctgcg gggcagccgc gccgtcttct ggctcgccag cgccctggcg ctgatcgcgt
     3061 tgaacgacag gcccggtggc agcatcaggc ccttctgcga gccggccacg gtgacgtcga
     3121 cgccccactc gtcgtggcgg tagtcgatcg aggcgagcga ggagatcgtg tcgaccatga
     3181 ggagtgccgg gtgcccggcc gcatcgatcg cctggcggac ggcggcgacg cggctggtga
     3241 caccggtcga ggtctcgttg tgcaccacca tgacggccct gatggcatgg cggcgatcct
     3301 cggccagccg cgcctcgacc agggccggat cgacgccgtg ccgccagtcg ccggcgagga
     3361 gctcgacctc gaggcccagc cgctctgcga gctcgcgcca gagcgacgcg aaatgcccgg
     3421 tctcgaacat cagcacccgg tcgcccggcg agagcgtgtt gaccagggcg gcctcccagg
     3481 cgccggtgcc ggaggcgggg tagatcacca cgtcctgctc ggtcttgaag acggtcttga
     3541 ggtcggacac gaccttgcgg ccgagggccc cgaactccgg cccgcggtgg tcgatggtcg
     3601 ggttgtcgat ggccctgagg acgcgatccg gcacgttcgt cggcccgggg atctgcagga
     3661 agtggcggcc gctcggggcg ttgctgaacc tgatcatggc gtctttcctg cattgcgctt
     3721 gcgtcgtcga ccgactgaac ctaggtcgga cgcagcgcac ctgtccattg gcggcgccgg
     3781 gggtgccccg cagggccggg ccggggagcc agagagcatg tcgaccagcg ccgccgatcg
     3841 gctgcccgcc accccgtcca gcgacgtgag cgagcgcctg gcgcgcctgc tgcgcagcca
     3901 gaccgagggc gaggtgctgt tcgacgcgcc gtcgcgcggc cgctatgcca cggacgcctc
     3961 gatctaccag atcatgccgg tcggcgtgct ggtgccgaag agcgagcggg acatcagggc
     4021 ggcgatcgac atcgcccgcg acctgaaggt gccggtgctg ccgcgcggcg gcggcaccag
     4081 ccaatgcggc cagaccgtcg gtgctgccct ggtgatcgac aacagcaagc atcttcgcgg
     4141 catccgcgag ctggatgtcg aggcccgctc ggtcgtggtc gagccgggcg tcgtgctcga
     4201 tcacctgaac gccgagctga agcggcacgg gctctggttc ccggtcgacg tctccaccag
     4261 cgcccaggcc accctcggcg gcatggccgg caacaattcc tgcggctcgc gctcgatcgc
     4321 ctacggcaac atggtgcaca acgtcgaggg catcaccgcc tggttgtcgg atggcgagct
     4381 ggtcgagctc gggccggtgg cggggctggg cggacgcggt gctgcgatcg ccggcttcgt
     4441 cgagcggctg gcggcgcagc atcgcgccga gatcgaagag cgctggccgc gggtgctgcg
     4501 ccgggtcggc ggctacaacc tcgacatctt ccacaaccgg agcgagcggc cctacaccgc
     4561 cgacagcagc gtcaatctcg cccatttgct ggtcgggtcc gaaggcacgc tggcctgcac
     4621 ccggagcctg acgctcagcc tctcggagct gccgcgcggc aaggtgctgg gcgtggtcaa
     4681 cttcccgacc ttccgggcgg cgatgagcgc ggcgcagcac atcgtcaagc tgggccccgt
     4741 cgcggtcgag ctggtcgacc ggaccatgat cgacctcgcc ctcgccaacc cggccttccg
     4801 gccggtcatg gaaaccgccc tgatcggcga gccggcggcg atcctgctgg tcgagttcgc
     4861 cggcgaggac aagacggcgc tggtgccgca gctggaccgg ctcgacgagc tgatgggcga
     4921 tctctcgctg cccggcagcg tggtgaagat ggccgacgac acggcgcaga aggacctctg
     4981 ggaggtgcgg aaggccggcc tcaacatcat gatgagcatc aagggcgatg gcaagccggt
     5041 cagcttcgtc gaggattgcg cggtgccgct cgagcacctc gccgactaca ccgacgcgct
     5101 ctccgaggtg ttcgcgcggc acggcacccg cggcacgtac tacgcccatg cctcggtcgg
     5161 cgcgctccac gtccgcccga tcctcgacat gcgcgaggcc gggccggggg gtggcgctgc
     5221 gaggatgcgc gccatcgccg aggaggccgc cgagctcgtg cgcaagtgca agggctcctt
     5281 cagcggcgag cacggcgacg gcctcagccg cggcgaatgg atcgcctggc agttcggccc
     5341 ggcgctcgtg gaagccttcc gcgccatcaa gcaggagctc gacccgatcg gcctctttaa
     5401 ccccggcaag atcatcgatc cgccgcgcat ggacgacagg gcgctcttcc gcttcgcgcc
     5461 gcccgacagc ctgcggccct accgggtcgt ccccctggag acgaagctcg actggtcggc
     5521 ctgggacgtc cggaacgacc cggtcacgga agcgaccacg ccgcccggca ccggcggtga
     5581 tcccgccggc ggcttcgcca aggccgtcga gatgtgcaac aacaacggcc attgccgcaa
     5641 gctcgatgcc ggcacgatgt gcccgagcta tcgcgtgacg cgcgacgagc agcacgtgac
     5701 acgcggccgc gccaacaccc tgcgcctcgc cttgtcgggc cagctcggcc ccgatgcctt
     5761 caccagcgag gcgacctacg agacgatgga tctatgcgtc ggctgcaagg gctgccggcg
     5821 cgaatgcccg accggcgtcg acgtggcgcg catgaagatc gagttcctcg cccactacaa
     5881 ggcgcgccac ggctacacgc tgaaggaccg gctgatcgcc catctgccgg cctacgcgcg
     5941 ggccgccagc cgggtcccgt ggctcatgaa cctgcgcgac cggctgcccg gtgctgcccg
     6001 ggcgagcgaa attctgctcg gcctctcggc ccggcgccgc ctgcccgaat ggcgcagctc
     6061 caccttctgg cgagcgaccg atccgtcgct gttcgcgacc cgggacgcga cgctcgccgc
     6121 cgctgccgaa ggcggcaagg cggcggtgct ctttgtcgat accttcaacg gcatgttcga
     6181 gagcgagaat gcgatggcgg ctgcccgcgt gctgcgcgcc gccggctaca cgttgcacac
     6241 ggtcgagaag gcgggggggc atcattgctg cggccgcacc tacctcgccg ccggcatggt
     6301 cgagcaggcg aaggcgaggg ccggtgcctt gatcgaagca ctcctgccgc tggcggaggc
     6361 cggggtcgcc atcgtcggcc tcgagccatc ctgcctcttg accctgcgcg acgaggcgct
     6421 ggtcatgggt ctcgggccgc gggccgaaat cgtggcgggg caggcgctgc tgttcgagga
     6481 gttcgtggca cgcgaggcca gggcaggccg cttcacgctc gagctgaagg cggccgaggc
     6541 acccatcctg ctgcacggcc actgccacca gaaggcattc ggcgcggtcg ccccgatcct
     6601 cgaggtgctg cgcctgatcc ccgagacgaa ggtcgagctg atcgaaagct cctgctgcgg
     6661 catggccggc agcttcggct acgaagccgg ccactacgac ctctcgatgc agatggcgga
     6721 agcaggcctg ctgccggccc tgcgccggca gccggacgcc atcgtggtgg ccgacggcac
     6781 gagctgccgc caccagatcg ccgatggcgc cgggcggcag gccctgcatg tggtgcggct
     6841 gctggagcgc ctgctgccgg cgacgacgaa gggcggttcc gcgtctgcct gagtctcttc
     6901 gggcgaccac acgggcagcc cgctggagcc tctttcagac gacccggacg ttcagggtgt
     6961 cgacgccctc gatgtgaccc accaggacgt cgccgcgctg cacggggccg acgccggagg
     7021 gggtgccgga catgatcagg tcgcccgcgg caagcgagaa atgcgcggag agataggcga
     7081 tcatctcctg cgtcttccag atcatctggt tgaggtcgcc cgcctggcgg agctggccgt
     7141 tcaccttcaa cgtaatggcg ccctgcgtcg ggtggccgat ctcgctggcg gggatcagcg
     7201 gggtcatcgg cgccgagcgc tcgaaggcct tgccgatttc ccacggccgg cccagcttct
     7261 tggcctcgcc ctgcaggtcg cgacgggtca tgtcgagccc gacgccgtag ccccagacgt
     7321 gatcgagcgc gtcctcgatg gtgatatagg ctccgccccg gccgagcgcg atcaccagct
     7381 cgatctcatg gtgaacgtcg gtgctcatcg gcgggtaggg aaactcgccg ctgaagtcga
     7441 tattgtcggg gttcttctgg aagaagaagg gtggctcgcg atcggggtcg tggcccatct
     7501 cgacagcatg ggccgcgtag ttgcgaccga tgcaatagat ccggcgcacg gggaacgtgg
     7561 cgctcgatcc gcgaacgggt gcaaccggct ggccgggtgt ctcgattacg tgttggctca
     7621 tagaggtctc cgaatctctg ctgcgcgcgg cgttcaaccg cggaaaggaa gttgtcaaag
     7681 gggggcgacg ccgatttccc tggcgatgtc gtccaggtcc ttgcgcaggg acgcatggag
     7741 cgcaatgccc ttcgcccggc gctcggccat cagggcagcc ttgccctcgc ccggcaggcg
     7801 caccctcgca tgtcccggca gcggctcgga cgcacgcatc tcgctgaacg ccttgtcgac
     7861 gctggcggtg aacgtgtcga gcgggccgaa cgccgcgatg tcgatcgccg cgacgaactg
     7921 gccggtattg ctgctgcgct gcgattcgct ggtgaagtcg atgacgtccg agccgaacgc
     7981 cgcctggttc aggaccccgg ccaggaggcc gatggcgacc gagaggccga agcccttggg
     8041 gccgccgatg ggcagcatga acccctcctc gcgccgcgac gaatcggtca ggggcttgcc
     8101 gtcgcgaccg accatccagc cttccggcat cgcctcgccg cgctgggcga gcgtcttgat
     8161 cttgcccatg gccgccaccg tcgtcgccat gtcgagcacg aacggatcgg cgccgccggt
     8221 cggtgccgcg atggcgatcg ggttggtgcc gaggagcagg tccgttccgc cgaacggggg
     8281 gacgtgattg gcgctgccga cggcagcggc catgccgatc atcccggcct gcgcctgcgg
     8341 gcggacatag agggctgccg gacccgcgtg gttgccactc cggacgccga cccagccgat
     8401 gccgcaggcc gtcgcctttt ccatggcgag gcggcacgcc gcccgcatgg ccaagtgacc
     8461 gaggccgtcg tcgccatcga tcaccgccgt agccgggcgc tcggcgacca cggccacgcg
     8521 cgccttcgga ttgcacccgc catccctgag gcgggccaga tactgccgca atcggaagac
     8581 gccgtgggtt tcgtgtccca gcagatcggc ctcgaccatc aggcccgcga cgatgttggc
     8641 gtcctcttcc ggaacatcgt agtggcgaag cgcgtcggcg acgaaacgct cgagccggtc
     8701 ggctgcgacg gtgggcgcct gttcttcact catggtcggc gagcacccgt tccttgcgcc
     8761 agaacacgat ccggcgctgg gcgaagctga cgagcacgaa gagtgccagg ccgagcaggc
     8821 tgagatagag gatcagcgag aacacgcgcg gcgtgtcgag ctgggatgcc gcgacccgga
     8881 tcagctcgcc gaaccctttt ccaccgccga ggaactcgcc ggtgatggcg cccgccatca
     8941 ccccgacggc accgatcttc aatccggtga agatctgcgg caaggcatgg ggcagcttca
     9001 tcttgaccag ggtctgcagc cggctggcgc ccatcgtctt gaacagcatg cgggcgttct
     9061 cgtccgccgc gtggagtccg gccgcggtcc cgacgacgat cgggaaggtg gcgatgaagg
     9121 ccgcgagcgc cactttcgac gcgatgccga atccgagcca ggcgatgaag agcggggcaa
     9181 aggcgacctt cggcatggtg tcgatcgcca ccagataggg catgatggcc cgctcgccga
     9241 agccggtctc gccgaccagc acgccgagcg agaagccgat ggcaatggcc agggcgaacc
     9301 cgtagatcac ctccttgatg gtgatccaca aggccggcag catgtagccg ccggtgatca
     9361 ggttgtggcc gacgaagatc aggtcctgga gcgtctcgcc gggagtcggc aggatgatcg
     9421 gcgaaaccag ccccgagacg gtcgccagtt gccacaagat cacgaacacc gcgaagacga
     9481 acagcatcga gagcgagcgc ggcacgcggt cgataaagga gatctgctcg gtccagacgg
     9541 tgtcgccttc ggtcggcgcc accacaggct gctgggtgct caaaggaatg cctcccggtc
     9601 caagaggccc cggatacggt cgaccagtcc gccgaacttc ggcgtcgtga tcatgtccag
     9661 cgtccggggg cgaggcagat cgatcgtcac ggcttcggct atccggccgg gacgcggctt
     9721 cataacgtag accacgtcgg acaggatgac tgcctcgggt atcgaatggg tgacgagaaa
     9781 cgcggtcgca tcgcgctcgg tgcagatgcg ctggagctcc atgttcatga agtcccgcga
     9841 cagctcgtcg agcgcgctga acggctcgtc gagcaagagg acggcgggct cggtgatcag
     9901 catccggcag atcgacgtcc gctgtgccat gccgccggaa agctcgccgg ggtagacgtc
     9961 ctcgaaaccc ttcagcccga cgacctcgaa cagttcacgc gcccgttccc tggccgccat
    10021 cgcagccgcg cgcccgtcgc ggatctcgat cggcaggacg acgttctcga gcgcggtgcg
    10081 ccagggaaag agcgtcgcct gctggaacat catgccgatg tcgcggcggg caccgatcac
    10141 gggctcgccc tgcagcacga cccgtcccgt ggacggggga atgagccccg ccatgatctt
    10201 gagcagcgtc gactttccgc agccgctcga gccgatgacc gcggagaagc ttcctttgtt
    10261 caggacgagg ctgacgtcct ggagcgcggt tacctggttt cgcgcatagg tcttgccgac
    10321 atgcgccagc tcgtagaccg gccgccccgt caggggcggc cgcatttcca gggccacgga
    10381 gccagtcaag tcagagaccc tggttccact cggcgacgaa ctcgttggtg tacgcctcgg
    10441 tgagatcagg cagcggctcc gccagctcgc cgccggcaat gaggttctcc tgccactcct
    10501 cccagacttc cggcggcatg tagcccaagc cgttcgacat gtcgaccggg atagtcttgg
    10561 accgcaccgc atcgaacagc gccgactgga attcggcatc ctcgccttcc tggggattgc
    10621 cggccttgag gtgcgccagc accttctcgc ggttggcgtc gtcgagcgcg aaggcatggc
    10681 cgcgcgcgaa cgcccgaccg aacttctcga ccagctcccg gtcgttgcgg atggtgtcgc
    10741 ccatcgtcgc gaggccgttg ccgaagaacc tcgcgaactc cgccggcgtg atgtcccgca
    10801 ccggcatgcc gcgctggttg aggatcgccg cgtcggcagt cgaggccgag taggccgcca
    10861 tggcaccgct ctggaaggcg accgtggccg gcccgccgtc gccgacggtc aggaactcgt
    10921 agtcctcgcc ctccttcatg tcggcgctga gcatgacgct gcgggcgaag ccgacctcgg
    10981 cgccgtcggc cgtgcccgtg ccgatcacct tgccctccag ctcctcgatg gattggatct
    11041 cggaatcgac cggcaccacg atgccgaaat tgctgcgtgc cgcgacgttg tagatgaaga
    11101 ccgcgtcgac cccgcgcgag cgcgcggcga gcaccggcgc cgggccgggg cgaccgaatt
    11161 ccgcctgtcc ggccgagagc gcctgcagca ccgcagcgga gccgttgatg gcctcgaccc
    11221 gaacgtcgag cccctcctcc tcgaagaacc cctcgccaat ggccacgaag accgggaacg
    11281 agttgatcgc cgacgggctg ggttgcacga acgtgatctg ccgcaagtcc tgtgccgtgc
    11341 cgacaccggc agcggcgctc aacgccaccg ctgcgccaac agcgatggag agttgtcgtc
    11401 gattcagcat catagcctcc attcgatcgt ttctggttgc atagtatcgg ccgcgacgag
    11461 tcgctgccgg cactattgga ctcggcagta gttctcgagg ctgccgatgt tttcgatggt
    11521 gcagacgacg acgtcgccgt cggtcaggaa ccgaggtgga tccatcgcgt aaccgacccc
    11581 cgacggtgtc ccggtcgcca ggatatcgcc cggctccagc gtcatgccgg ccgacagcga
    11641 agcgatcagc gtgggaatat cgaagatcat ccgggcggtc gtgctgctct ggcgggtctc
    11701 gccgttgacg gtgaggctga tcgccaggtt gccaggatca ccgatttcgt ccgcggtgac
    11761 gatgcagggc cccatcgggc aggagccatc gaggcccttg cccttgaagt actggccgcc
    11821 gtggcgacgc tggatgtccc ttgccgagac atcattgatg atcgtgtagc cgaggatgtg
    11881 gtcgaaagcg tcctcgcggg cgatgtcccg gccgcgcctg ccgatcacca gggcgagctc
    11941 gacctcgtag tcgatcgacg tcgagacgtg cgggaagatg ggaatcgcgg cccccggccc
    12001 gatcaccgcc gtgcgcggct tggtgaagaa gacgggatgc tcggtgacgc cgaccttctg
    12061 gttctgggcg cgatcgcctt ccgcgatatg ctcggcgtag ttgcgaccga cgcagaagac
    12121 gttcttcgcc ggtgacggca ggggggccat ggtcacatgg tcctcgggcc ggcgctgcgc
    12181 ggaccgatcc gagcggtcgg cgagcgttcg cacgaggtcg agcgccttgg gtccagcctc
    12241 gatgatggat tgcaggctgg ccgtgcggac ggcctcgccc gcagcgcccg gcgcctcgcg
    12301 ggcggcttcg gccagatcga gcacgttgcc gttggccaga accacgccgg ctcgcggcgc
    12361 cccatcgatc aagagcgtaa ccaggcgcat ccaaactcct cccggcacgt cgaaccgtct
    12421 accgataggc cttctcgaca ggccgtcaac cgacgatctc tgaaggacgg attcgacgcc
    12481 gatggctcac gatcagagcc cccatttatt ggatgatcag attgcgtttc gatctaggat
    12541 caggcaacca accaatgtga tgattgggtg aaaatgagcc ggtccgaccg tcacgcactc
    12601 gccgagcgtg tcaggacatt catcggcgag catggctacg accacaacca gcgactgccg
    12661 ccggaacgcg agctttgcgc cttgctggac gtttcacgga gccagttgcg caatgccctc
    12721 gcggtcctcg aggagaacgg cctgatctgg cgtcatgtcg ggcggggcac attcatcggc
    12781 gcccgaccgg tcctgaacct cgatgacatc accttcctca gagagctcgc gagccccgag
    12841 cagattctgg agtcacggac ggcgatcgag ccggcgctgg cacaactcgc ggcaatgcat
    12901 ggcacgaagg cagacctcga ggagatcacc gcctgcgccg cccgctgccg caacgctgcc
    12961 gactggcgca gctatgaagc gtgggacaac aatctccacg acgcgatcgc caaggcaacg
    13021 cacaacaagc tgctgatcta ccttttcgag acgctcaacg tcgtgcgccg cacggtcgtc
    13081 tggggtcaga cgcgcacgac acggggtcca aaggccgacc atgtgagctt cacggaacat
    13141 gatgccatca tcgccgccat caaggtgcgc gacgccgacc aggcggcatc ggcgatgctt
    13201 gcccatctgc gctcggtgcg ggagcgcgtt ctgccgatgt tgattctcag ggacgccggg
    13261 gaccggcgca cgctcttcga gcaacaggcc gcgccggcgc ggtgacggtg gtgccgaaag
    13321 gtcaaagcgc ttttccgcag cattcggaac tggacgcccg aacgcgctcg atcgacaggg
    13381 atacttgcgc cagaagccgg cgcaacttcg ggacactccg gcttcaggcg tgaggccatg
    13441 gttgcggttg acgcccgttc ggccgtcgtc tcccggtcgc atccattgag acgacgaatg
    13501 tgccaagccg tggcgcccac cccggtatgc caggacggca attttcggaa atccggctga
    13561 ctccctgaaa agacgctgga tcgcgacctg taaattccat acttcagacg tgcgcacggt
    13621 cgacgttcgc taatcaacga cagggaagaa ggcgaccatg agaatgagaa tgacgattgc
    13681 ggcagcggca atcgggctgg ccttgtcggc gcccgccgca tttgcggaca agctcgacga
    13741 tatcatcgcc tcgggtgtgc tgcgctgcgc cgtggtgctg gatttcccgc ccatgggctc
    13801 gcgggacgcc aacaacgagc cgatcggctt cgatgtcgac acctgcaacg acctggcggc
    13861 ggcgctcggg gtcgagccgg aatatgtcga gacgccgttc cccgaccgca tcccggcgct
    13921 ggtgtccggg cgtgccgatg tcggcgtggc atcgacctcc gacacgctcg agcgggcgaa
    13981 gaccatcggc ttctcgatcc cctacttcgc cttcgagatg gtggtcctca ccaaggacgg
    14041 gctcgggatc gacagctacg agagcctgaa gggccacaag atgggctcgg tctccggcac
    14101 gttcgaggcg atccggctcg agaacgacgt cgacgcctgg gccgacgcga acggctcgtt
    14161 cacgccctac cagacgcagg cggacgtctt cctcgccttg tcgcagggcc agatcgacgc
    14221 caccgtcgtc acctccaccg tggcgaacgc ggctgccaaa tccggcgact tcgagggctt
    14281 cgtggtgggc ggcaaggcgc cctacgacat cgactacacg gcgctcattg cgctccgcga
    14341 ggagtacggg ctgctcaact atctcgacct cttcgtcaac cagcaggtcc ggaccggccg
    14401 ctatgccgag ctctatgcca agtggatcgg ctcggacgag ccgccggacc tgacggtccc
    14461 cggcgtctat cgctgaccgg caggcatgtg gcgagggcgc gccgccggcg cgccctcgcc
    14521 acgaccgggc cggtcggcgt gaacgtgtga tcgtgccgga ggtcgcctga atgaactaca
    14581 ccttccactg gcggccggcg ttcagggccc tgccggagat gctcgccggc tcgctggtca
    14641 cgttgcagat cgcggtgctc tcgatggtga tcggcattgc gatcgccgtg cttctggcgg
    14701 tgatgaccgg gtcgaagagc cgctggctgc gcggcattgc cggcacctgg atcgaggtgg
    14761 cgaggaacac ccccgccttg ttccagatct acatcgccta tttcgggctg gggcagatcg
    14821 gcatccatct ggatagctac ctggcgctgc tcgccgggat caccttcaac aatgcggggt
    14881 atctggccga gacgttccgg ggcggccttc gggccgtgcc gcagacgcag gtgcgcgccg
    14941 cccgctcgct cggcttcggc ccaatcgccg cgttccggct gatcgtcctg ccgcagctct
    15001 ttcgcgccgt cttccacccg atgaccaacc agatggtctg ggcgatcctg atgacgtcgc
    15061 tcggtgtcgt ggtcggcgtc aacacggacc tcaccggcgt cacgcaggag ctcaacgtcc
    15121 gcaccttccg cacgttcgaa tacttcgcca tcgcggctgt gctctactac ctcatcgcca
    15181 aggtcgtcac gctgggtgcc cggctgatcg cctggcggct gttccggtac taggatgctc
    15241 gatacagcgc tctccctgaa cgatctgcgc ttcttgatgc tgggcgccgg ctggacgctc
    15301 ttgatcaccg ccatctcggt caccgccggc accctgctcg gtgtgctctt cggtgtggcc
    15361 agggcgcaga cgcccgtctg ggtcagcctg ccgatcggct tcgtgctcga catcttccgc
    15421 tcggtgcccc tcctgatcca gctcgtcctc ttcaattcct tcatcccgat cctggggttg
    15481 cgggcgacgc ccttctccgt gagctgcctc gtgctggcgc tctacacctc ggcctattgc
    15541 tcggagatcg tgcgtggcgg cgtcgcggca gtgccccaga cgacgcgccg ggcggcgcgc
    15601 tcgctcggca tgacctgggg gcaggacatg gtcgagatcg tctttcccct ggcgcttcgg
    15661 gtgatgtttc cgagctggat cggcctcacg ctcggcgtca tgaaggacac ggcgctggtg
    15721 gcctggatcg gcgtgatcga gctcttgaag tcgtcgcaga tcttgatcac gcggatgccg
    15781 agcgaaccct tgttcattct cggcattgcc gggctgatct acttcctgat cagcttcccg
    15841 atatcccggc tgggagccag gctcgagcgg aagtggcgcg aaaatgattg agatcgagaa
    15901 cgtccgcaag agcttcggcc agctcgaggt cctcaaaggc gtgtcgctgg ccgtcgaccg
    15961 tggcgaggtg gtgtcggtga tcggtggctc cggctcgggc aagtcgacgc tgctgatgtg
    16021 catcaacggc ctcgagccta tcgactccgg acgcatcctg gtcgacggcg tcgaggtgca
    16081 cgcgaggtcg acggatctca acaagctgcg ccgcaagatc ggcatcgtgt tccagcaatg
    16141 gaacgcgttt ccgcacctca ccgtcctcga gaacaccacg ctggccttgc gcaaggtgct
    16201 gggaaagtcg cgcgccgagg ccgacgccat cgcgatcaag cagctcgagc atgtcgggct
    16261 gcaggacaag ctcaaggtct ttccgggcaa gctctcgggc ggccagcagc agcggatggc
    16321 gatcgcgcgg gcactggcga tgagcccgag ctacatgctc tttgacgagg tgacctcggc
    16381 cctcgacccg cagctcgtcg gcgaggtgct cgacacgatg cgcctcctgg ccgacgaggg
    16441 catgaccatg atcgtcgtca cccacgagat gggcttcgcg cgcgaagtgt cgaaccgggt
    16501 cgccttcttc aaggacgggt tgatccacga gatcggcacg ccgaagcaga tcttcgagca
    16561 gccgcagcgc cccgagacgg cggaattcct gggttccgtc ctttgaccat ccgccccgca
    16621 tcgaggacag cgccatgaag atcgcggcca tccgcgtcta ccagatcgac ctgccgctga
    16681 aggaaggccg ctacagctgg tccaacggca atttcgtcga ggtcttcgat tcgacggtcg
    16741 tcgaggtcga gacggattcg ggcctgaagg gctacggcga atgctgcccg ctgggctccg
    16801 cctatctgcc ggcctatgcg ctgggcgtgc gctccgggct cagggagctg gcgccgcagg
    16861 tgatcggcat ggacccgcgc gacctgcgcg tcctgaaccg gcacatggac gcggtcctgc
    16921 gcggccatcc atatgtgaag tctgccatcg atgtcgcctg ctgggacatc ctgggccagg
    16981 cggcggggat gccggtctac aagctgctcg gtggcgcggc acaggcggat gtcaggctct
    17041 accgggcgat ctcgcaggag gcgccggagg agatggcacg caagatcgcg ggctaccggg
    17101 ccgagggcta cacaaagttc cagctcaagg tgggcggcaa tgcggacgac gatatcgagc
    17161 gcattcatgc ctgcgccggg ctgatgcagc cgggcgacat cctcgtcgcc gacgcgaaca
    17221 ccggctggac gatgcacgag gcggcacggg tcgtgcaggc cgtgcgcgac atcgacgtct
    17281 atatcgagca gccctgcccg acctaccggg aatgcctcgt gacgcggcga cggacggccc
    17341 tcccgttcgt gctggacgag gtgatcggcg acgtcgacac gctgatgcag ggcatcgccg
    17401 acggggccat ggacgtcatc aacctgaaga tctcgaaggt cggcgggctg accaaggcgc
    17461 tcctgatgcg cgacatctgc actaccgccg gcattccgat gaccatcgag gatacctggg
    17521 gcggcgacat cgtcacggcc tcgattgcgc atctggccct gtcgacaccc gaggagttcg
    17581 ccttctccgc gacggacttc aactcctacg gcacggtcga gttcgccagt ggtgcgccca
    17641 ggcgtgtcga tggccgcatg cgcccggccg aggcgcccgg gctcggcatc gagccgatga
    17701 tggacgtgct cggcaagccg ctgctcaccg ttccctgagg aatcgaaggt gcgctcctcg
    17761 aaggttcttc atgtcgtcag ctgccatgcc gagggcgagg tgggcgatgt catcgtcggc
    17821 ggcgtgccgc ccccgcccgg tgccaccatc tgggagcagg cgcggtggat ccaccaggac
    17881 cagcgcctgc gcaacttcgt cctgaacgag ccgcgcggtg gcgtcttccg ccatgtgaac
    17941 ctcctggtgc cgcccaagga cccgcgtgcc gtgaccggct ggatcgtgat ggagccggtc
    18001 cacacgccgc cgatgtcggg ctccaactcg atctgcgtcg ccaccgtgct gctcgatacc
    18061 ggcatcgtgc cgatggagga gccggagacc cgcttcgtca tcgaggcgcc gggcgggctg
    18121 gtcgaggtgg tggccaattg ccgaaacggc aaggcggagt ccgtacgggt ggtcaaccat
    18181 ccttccttct cggcccgcct ggacgccatg atcgaggtgg aagggctggg cagcctgaag
    18241 gtcgacacgg cctatggtgg cgacagcttc gtgatcgtcg atgcggctgc ggtcggcttg
    18301 gagattcggc ccgacgaggc gttcgacatc tcgcggctcg gcatgcggat cacgagagcc
    18361 gcaaacgagc agctcggctt cacgcatccc ttgaacccgg actggcgcca catctccttc
    18421 tgccagttcg tgaaccccgt ccgcgagatc gacggtgtcc cgaccggccg gaacacggtc
    18481 gcgatcgagc cgggcaagct cgaccgctcg ccctgcggca ccggctgctc ggcccggatg
    18541 gcggtcctgc acgcccgggg gctcctcggc cagggcatgc ggtttcgcgg catctcgatc
    18601 atcgattcgg tcttcgactg ccgcgtcgag cgggagacgg aggtcggcgg cctcgccgcc
    18661 atcgtgccga gcatccaggg ccgcgcctgg atcaccggga cgcaccagca catgctcgac
    18721 ccggccgatc cctggccgga gggctatcgt ttgtcggaca cctggcccat ccgggccgtg
    18781 cccggctcac agcaagaagg aaccgcgtga atggacatct ccgtcttcaa aggctgcatg
    18841 ccggccctga tgacaccctg cacggccgac cggcgcccgg atttcgacgc gctcgtcgat
    18901 tgtggccggc ggctgatcga tgccgggatg cgggctgtcg tttattgcgg ctcgatgggt
    18961 gactggccgc tcctgaccga cgccgagcgg caggaggggg tggcccggct ggtcgcggcc
    19021 ggggtgcccg tggtggtcgg cacgggtgcg cagagcccga aagccgcggt ggctcacgcg
    19081 gcgcatgccc agaaggtcgg tgcgaagggc ctcatgatga ttccccgcgt gctgtcgcgc
    19141 ggcagctcgc tagccgccca gcgggcacat ttctccgcca tcctggctgc cgcacccgac
    19201 ctgccggcgg tcatctacaa cagcccctat tacggcttcg ccacccgcgc cgacctcttc
    19261 ggcgagctgc gccgcagcca caagaacctc gtcggcttca aggagttcgg cggggccgag
    19321 gcgatgacct atgcggccga gcacatcacc ggtgcggagg acggcctgct cttgatggtc
    19381 ggggtcgata cctgcgtctt ccacggctat gtcagatgcg gtgccgtcgg cggcatcacc
    19441 ggcatcggca atgccctgcc ggccgaggtc ctgcacctca tccggctctg cgagcaggcg
    19501 cgcgagggcg acgtcgatgc gcgccgcaag gcccaggagc tcgaatccgc cctggcggtg
    19561 ctatcctggt tcgacgaggg ggccgatctc gtcctctatt acaagtacct gatgacgctg
    19621 gaaggcgacc ctgcctacga gcacaatttc gtcgagaccg atgagctctc ggcgagccag
    19681 aagcacttcg ccaggacgca gctcgcccag ttcaaggcat ggtacgccca ctggtcgcgg
    19741 ccgcgctgac ggttctgccg aggtctgtca gtcgcggagc ggcattgcca ccgggcgcat
    19801 cggggcgccg gtggcgccgc gcagcctgat gcaggcgccg aagaaggcga actcccatag
    19861 cttgttggca ccgatctcct cgaggttgac cacctccatg atcgggatgc cggcctcggc
    19921 caggagatag ctgtggacca ccatccagtt ctcgggatcg gccgagggcg tctgctcgag
    19981 cgccagggtg tctgaaccga tggtgatggc accggccttg gccaggaact cggcaccggc
    20041 ccggttcagg ccgggtgcgt tgagcgtgaa cgcatcctgg ttcggccagg cccgcatgcg
    20101 cccggtccgg atcatcacca cgtcgcctgg ccgcagctcg acggcctggt ggcgcaggca
    20161 ggcagcgagg tcgacggggc cgacgggatg gctgtccggc agcatgtcga caccatgcat
    20221 cccggcgatg tcgaggagca cgccccgggc cagcatgggc gggtgcttct cggcgccgca
    20281 gacgcgccag tggcggctgc cgagatgctc gtcggcgtgg aagtgattga agatctcgcc
    20341 gtggtagccg aagtggttca gcgtatcgat atgcgtgccg caatgggtgt acatctgcac
    20401 gacatcgccg gaataggaga cgagctcgtt ctgggtccgg ccgaccccca tgacgtcgtc
    20461 ggcctcctcg cccctcggcg tgtgggtcat ccagatctgg tagggcggat cgccggcccc
    20521 gaaccagccg ggcatgccca tgaagtggtc gaccgagaga tcgaacatcc gcccggcatc
    20581 ggcgcgggcc atgatggcct gggcggatgc cgcgtccatc aggtttagca tgccgatctc
    20641 gtccctggca ccgaacgggc tgctcgatgc ctggcgaaag ctggcaccgt gcgccgcgat
    20701 ccggcgccgc gtcacctcgt cgacgatctg gctcgtgcac atgggcgaat cctcttgcgg
    20761 cggttcgacc tggtggcccc taggtcgtgg acgggtagtc gaccggggtc gcgtgccagc
    20821 cctgccggcg gcgctcccag aaggtttcgc gcaagttggc ggagatcggc ccgggccggt
    20881 cgttgccgag aatccgcccg cccagacggc tgaccggcat caccccgccc gcggtggtcg
    20941 agaagaagat ctcgtcggcc tcctcgagct cgtccgccga tagcggtcgc acctcgcagg
    21001 gaatctggag cccttcgcag agctcgaaga cggattgccg ggtgatgcct tccagcgcgc
    21061 cttccggcgg cgagacgact gtgccgtcga tcaccgcgaa gacgttgtag cccggcccct
    21121 cggtgacgtt gccggcacca tcgagcagca cggcggtgtc gaagcccgca tcctgcgcct
    21181 cgaagagggc cgccgtgagg tcgccccagt ggaagttctt gaccgtggga tcgaccgagg
    21241 cggcagggat gcggggcgtt ttcgcgatca tcgcatgcac gccgcgggcc tgctgctcct
    21301 ccgagaagag ccagacccac ggcacggcga agcagacgag ataggcgcgc gcgttgaccg
    21361 gcagcatggg catgccgcgt ggcggtcggc tgcgcaggca gtccatcgcc acataggcct
    21421 cgcgcaggcc cgtccggcga acgagctcca tgaggacttc ccggatctcc gcgtcggact
    21481 cgccgggctg catccgcatc cggtccatcg agcgccggaa gcgggcgatg tggtcgtcga
    21541 ggcggaagaa ggcgccgaag aagacgccgg tcacgtcgta ggtgacatcc gagcggcgat
    21601 agccccactc ggtgatcggg atcctggcct cggccagggg catgaagcgg ccgtcgatat
    21661 aggcggcacc agccgaccag tcgaccatgg cctcgccttc catcatgtgt cctttccatc
    21721 gccggcgtcg gcccggagcg cccgggtcgc cgccctgtcg atcaccatgc cagtcccctc
    21781 cgcctcgatc accacgccat agacccgccg tgcccgttcg gtcgagatcc agccttcgag
    21841 cacatcgtgg agcacccttt cgggatcgcg gctggcgggc gcgccatagc cgccgccgcc
    21901 cgccgagtgt gagacgacct gctcgccggc gaccagggtg acgccatggc aggcaggcag
    21961 cgtcgtcagg ctgccgtcga gctcgcgctt gaaggcctcg gcccggcagc cgggccagcc
    22021 gccacgcgca ccctgggccg ggttcaccgt gccgtcggca gtgtagagca cctgcatggt
    22081 gcagccttcg atcggcccga actcgacata ggccgagggc gccccgcgga actcgccggg
    22141 tccttcgctg tcggtcagga gccggcgatc cttgacgaag atggggtggt gcagctcgtc
    22201 cacctcgatg ctgtcgtgtc ggcagagccc ggcattgccg acatgtatga tcgtcagaaa
    22261 gccgtcgctc tgcggcgtgc cggcgccgcc ggtgatgccg aggaagatct ggttgacgaa
    22321 gggctcgccg gtgcgcgggt cgcggccgga gatcacgccc atgccgggcg gctggatcgg
    22381 tccggtctcg gccatgccga agccgggggc gatctcggcg attgcccgct gcaccgggct
    22441 ggcgactcga tcggcgagat tggtggtcgc caccgagcat gaatgggggt ggcgggggat
    22501 gccgacgacg cagttctcgc gcagcttgat gtcgatgcgg cgaaagctgc cagcattggc
    22561 cggcacggta tggtcgatga tgccgttgta gatgccgacc atggcagccg tgcgggcgca
    22621 gccctcggag agattcaggc cgcagggctg gcagtcgata ttgtcgcgca ggtcgacgct
    22681 gatcatggcg ttctcggcgt cgatctcgac gtcgatactg accgggatgc catcgggcac
    22741 gccggggaag ggatcatgcg ccgactgcac acggatccgg ccggacggca gacgccggat
    22801 ggccgcgatc atcttgcgct cgcagtagtc gaaccaattt tcggtatagc ttgcgagcgt
    22861 cggccagccg acgtcccggc cgagctccag cagctcccgc tcgccgatcc ggacggcgcc
    22921 gagcgttgcc agatagtcgc cccaccattg ctccgggaca cggatgcgag cccggcacat
    22981 gcggatcaga tcgtcattgt cccggtagcc cgtctggatc cgggccgccg agaagatcag
    23041 cgcaccctcc tcgtagacgt cggtggcgtg gcccatgtag gtcgtcggcc gggagttgcc
    23101 gcagtcggcc tggtgcgcct tggcgaggac gaagaagcgc agcgtcgcgc cctcgtccac
    23161 cacgggcacg atcaggcagt gatcggccgg gtgcgaatcg ccctcatagg ggctgttgtg
    23221 gagaaagcag tcgccggcgc tgagcgtcgg atggtgccgc ttcaccgcct cgcacatcag
    23281 gtctgggccg atcagcgtgt ggatcggcag gctctcggcc gaacccagca ggcggcaatc
    23341 ggcggtgagg atgacgcacg agaaatcatg ggccgtattg aggacgcccg accgggccgt
    23401 gcggaagagc gtgttctgca tcttccgggc gatgctctcg atgcggttcg agatcatcgc
    23461 catgcgcacg ccgtcccgac gccagccggc gccctccatc tcggctgggg tggcctgtcc
    23521 ctcgttggcg tcgttcatcg ctggtttccc atgccgtcag gatccggtgc gcaagtcggc
    23581 gcgccgctgg tcggtcgccg cgaggtcgat ggcgagcctg tccgcactgg cctcgccagt
    23641 cagcacgacg ccgtagacag tgcttgcccg ctcggcgctg acccagccct cggcgacatc
    23701 gcgcagcacg agctccgggt cgcggtcgag cggcgagccg tagccaccac ccgccgcgga
    23761 atgggagacc acccgctcgc ccggggccag ggtcagcccg tggcaggccg gcagctcggt
    23821 cagcgagccg tcgcgattgc gtctgaaggc ccgcgacggc gccccgctca ggccgccgac
    23881 ggcgccggcc gcggcgttga tcgagccgtc catggtgtag agcacgcgca ggttgcagcc
    23941 ctcgatcggt ccgtactccc cgtggatcgc caaggcaccc cgatgctcgc cggcaccctc
    24001 ggtgtcgggg acgaggcgtc gggtggtgac gatgatcggg tggtgcagct cgtcgacctc
    24061 cacgcagtcg atgcggctga gcccggcatt gccggcgtgg atgatggaga ggaacccgtc
    24121 gctcgtcggg gtgccggcgc cgccgctgat cgctagatgg agctggttga cgaagggctc
    24181 gtcgccatgg cggggatccc ggcccgagat gacggcgatg ccgggcggca tgatcgggcc
    24241 ggtctcggcc atgccgaagc cccgggcgat ctcggcgatc gcccgctgca ccgggttggt
    24301 cacgcggtcg gcgaggttgg tggtcgccac cgagcaggaa tgcgggtggc gcgggatgcc
    24361 gacgcagcag ttctcgcgca ccttgatgtc gagacgccga aagctgccgg cattagccgg
    24421 cacgctgtgg tccatgattc cgttgtagat gccgaccatc gccccgctga ccgaggtccc
    24481 ctcggtgagg ttcaggcccg agggctggca gtcggggttg tcgcggagat cgacggtgat
    24541 ccggccggct tcggcgtcca cctcgacctc gaccttgatc gggatgccgt tgggcaggcc
    24601 ggggaagggg tcgtgcgccg tccgcacggt cgccttgccc gacggcagcc ggcggatcgc
    24661 ctcgaccatt ttccgctcgg aatagtcgaa ccaggcctcg gcatagtcgt cgagggcggc
    24721 gaagccgacc tcgcggccga gctcgaggag tccacgctcg ccgatccgga ccgagcccag
    24781 ggcggcgagg tagtcgcccc accactgctc gggcacgcgg atgcgcgccc ggcacatgcg
    24841 gatgatgtcc tcgttgtcct tgaaatcgga ctggatcctg gtcgccgaga aaatgagcgc
    24901 accctcctca tagacgtcga cgacatccgc gagataggtg gacggccggg cattgccgca
    24961 atcggcctgg tgcgccttgg cgaggacgaa gaaccgcagg acctcgtcgt cgtccattac
    25021 cggcacgatc agacagtggt cagcggcgtg cgagttgccc tcgtaggggc tgttgtgcag
    25081 gaagcagtcg ccgcgccgaa gcaccggatg gtggcgcttg accgcctcgc acatcaggtc
    25141 ggggccgatc agggtgtgga tcggcaggct ctcggccgag cccagcaggc gacagtcggc
    25201 tgtgaggatg cagcacgaga aatcgtgcgc cgtgttgaga atgcccgagc gtgccgtgcg
    25261 gaagagcgtg ttctgcatct tgcgcgcgat gctctcgatc cggctcgaga agagcgccat
    25321 gcggatcgcg tcatggggcg tcccggtcgc ggcggtggtc atcggccggg tcccatcaga
    25381 acgttacccg caggccaccg ccagtcgtcc ggacggcgac cgtcccgggg tcgaccacga
    25441 cggtcgtgaa cgatgattcg acgatggccg gcccgggcac tggctcgtcc gccggcatcg
    25501 cctcgaagcg ggcgacccgg gcctcgaccc agccggagcc ggcgaaatag acccggcggc
    25561 tgaccggcag gtcgctctgg ctgtccggcg gcggcacccg gccggtcgcc gcctcgcgca
    25621 gccggcagcg gaccttggcc cgccaagtga cgaactcgac ctccgagtgc ggatcggaga
    25681 tctcgaagat ggccttgtgc gtgtcgtgga aggcggactt cagcgcggcc agggcttcgg
    25741 gcccgtcgac ccgccgcccg gcgagcggca cctcgatctc ccagatctgg tgcggatagc
    25801 gggcctccac cgagaactcg atcagtgtct cgagggagcc ttcgcccggg ccgtttgcga
    25861 aggcctcgca gcgcttctcg agtgccgcca gcacctcgtc gactgcctcg taggcgaaat
    25921 tctggctcgt cgtgaactgc atccgggcgt agtcggacgc cagctcggac atgagtgcgc
    25981 cggcagcact gaggacggcc cccgcctcgg gaatcaggac accggcgcag ccgagccgcc
    26041 ggccgaccgc cacggcattc aggccggcag caccgccgcc gccgatcagc accgccgatg
    26101 acggatcgat gccctggttg acggtgatct cgtcgatggc gccgaccatc ttctcggtgg
    26161 ccagcgccag cacggccgct gccgcctcct ctgccgacag gccgagcttc gccgccacat
    26221 tggtctcgag ggcggcaacc gccgccgcgc ggtcgagcgc catcgccccg ccgaggaaga
    26281 aatcggcgtc gatatggccc aggatcagcg agcagtcggt cacggttggc tcggtgccgc
    26341 ccttgccgta ggcgaccggg cccggcgtcg agccggcgct cttcgggccg acccgcagca
    26401 gcccgcccga atccacccag gcgatcgaac cgccgccagc gccgatgctc ttcacgtcga
    26461 ccgacgggaa gccggtcatg tggccgcgga agcgctggcc gagccaggtc tcgcgggtcc
    26521 aggggatgcg cccgtcgcgg accaggctca ggtcatagct ggtgccgccg gtatcggcga
    26581 cgatggccat ggcgttctgc tggtcgcgct gggcgtagaa gcggccggcc accggcgcca
    26641 tggcggggcc cgagttgatc gaatggatcg gcgccctggc cacgtcctcg gcgtccatga
    26701 tcccgccgcc cgacgtgacc atcaaggttc gcccgttgaa cccggcctgg cgcagccggt
    26761 cggtcaggcc gttgagatag gcgaacatga gcggcttgag cgaggcgtcg atgacggtcg
    26821 ccgaggcccg gcgatactcg cggagcgtcg gattgaggac atgcgagagc gtcaccgaca
    26881 cgcctggcag gtgctcggcg aggagttcgc cgacccggcg ctcgtgggcg ccgtcgacca
    26941 ccgaccagag caggcagacc ccgacggcct cgacctcgag cgccttgagg cgctcgatga
    27001 tggcgagcac cgtcgcctcg tcgagcggct cgacgacgcg gccggccgcg tcgatgcgct
    27061 cgggcacctc gaaggtgagc cgccgcggca cgtagggctc ggggtagggc acggtgaaat
    27121 tgaagggctc gatacggccg ccctcgcgga tcaccaggac atcgggatgg cccttggttg
    27181 tcaggaaggc ggttctggcc gtgttgcccg tcaagatggc gttgatggcg cgcgtggtgc
    27241 catggatgaa ggtggtggcc tcggccagga aggctgcccg gtccaggccg tgcgccgcgg
    27301 ccgccaggcc gagcacgtcg agcacgccct gcaccggatc ggctggcact gtcgatgact
    27361 tgaactggcg gatctcgtca ccgcgctcga ccacgaggtc agtgaacgtt ccaccagtgt
    27421 cgcatgccag ccgcatggtt gcgtttcccc cggaaggccg ataatgctga ggcgtctgtg
    27481 atcacagcct agatcgagcc tcgggctcag gcaatacggg ttcgattgtc gtgatcacag
    27541 agtgtgcggc gcgtgccgtg atcggccgat ggccggcggc accgacaccg tgccggcaac
    27601 gtcggagggg acgctggaat gaacgccaag gtcgttcgcg tcggcgaagc ggcacgacag
    27661 ccggccatgc ggggcatcga cctggtcgcc gacctcgtgg ccggcaccat gggaccggcc
    27721 ggccgggcag tgctcgtcgg ccgcaaccac gcgccgccgc tgctcctccg caacggctat
    27781 gccatcgccc ggaatctcga gctcggctcg cgtgacgagc aggccggcgt cctgctcatg
    27841 cgcgatctcg cgtggcgcac cagcgatgct gtcggcgatg gcaccagcac ggctattctc
    27901 gccgcccgcg ccttcgtccg ggccggccgt ctcgccgcac tttcgggcat cgcgccgagc
    27961 gagctgcagg ccgcgatcga tgcccacagc acggccgtgc tcgaagcgct cgaggccgcg
    28021 tcctgcccgg cgccggaggc cgcggtgctg gagcgcgtcg ccacccaggc gctcggcggc
    28081 gacgctgccc tgggtgccct gctcgccgag gcgcatctcc aggccggcag cgatggcatg
    28141 gtggtcatcg aggagggccg gagcgcggag gacaccctgc gcttcgacgc cgggctgcat
    28201 ttcgaccagg gctggatctc gccgcatctc gtcgacgatg cgcgcaccag gacgatcgag
    28261 atcgagaacc cgctgatcct cgtccatgcc ggcccgatca ccgccctgca gcccgtcgtc
    28321 cgggtgctcg agatgatcgc cgaatccggc cgcggcctcg tcatcatcgc cgattcggtg
    28381 gccgaggacg ccctgaccac cctgatcgcc aacaagcggg gggctgggct gaaggtggcg
    28441 gcgatcaagg cacccggggc gggtgcctgg cggtgctcga ccctcgagga catcgccatc
    28501 gcgacgggtg ctgcgctgat cgccgaggag ctcggccatt cgctcgccgg gctgcggccg
    28561 cagttcatgg gcggtgccgc cagggtcatc gttcggcgcc atgattccac gatcatcggc
    28621 ggcaagggcg accctgccgc cgtggcgacg cggaccatgg agatccgcga tgccatcacc
    28681 cgcgagaagc acctgagctt cgatcgcgag cagctccgca agcggctggc gcgccttcag
    28741 gcggggatcg cgacgctcgg catcggcggg cggacaccga cgcacctgca acttcggctc
    28801 gagcaggcgc gaaacgcatc ggcgaccctg gcagcggcgc tttccggcgg gctcgtgccg
    28861 ggcggcagcg cggcgctggt gcatgccgag cggcgcgccg cggcggcctt gccggagggt
    28921 ctcctgggat cgctgctcgg ccgcatgttc gccgccgcgg ccgccgctcc cttgcgtgaa
    28981 gtggtgcgca atgcgggtgc ggacgccgat ctggccctgg gcaggatcgc cgccgatccc
    29041 ggcacgacct tcgacgtccg gagccgcgcg atcgtgccgg tcgacaccat catggatccg
    29101 gcgcccgtgg ccgttgccgc gttccggggg gctgtctcca ccgcgagccg actcctcggc
    29161 gtggcggcat ccttgcatga ggccggacat gcatccgatc gcaccacggc attggactag
    29221 gtcgatgatc actcgggcca cggtatcgac actgatcgac ccgcaccgtc ggcgtccggt
    29281 ctcgcctgga ggctgcctgt cgggcgggca cgctgaccgc cgtttgggca tccgtgatca
    29341 cagatggagg tgtgtgccgg cccgatccca gccacgtcga acggagagca tctgatggcg
    29401 cagttggctg cccgtctggc tacgaagccg gttcaaaagg ctgccaccgg ggccggtgag
    29461 gaggactcgt cgccggcacc cgagaccgtg ggcaagccga tcctcgaggt catcgacgcg
    29521 gtgcatgtct atggcgacgt ggtagcgctc gacggtgtca cggtgtcggc cgcggccgga
    29581 gaattcctga ccatcctggg tgagagcggt tccggcaaga ccacactgct caggctcatc
    29641 tcgggactcg agaagcccta ccggatcggt gccttgcgcc tgggcggcgt cgatgtcagc
    29701 acggttccgg cctatttgcg caactgcact actgtcttcc agcactacgc cttgttcccg
    29761 cacatgtcgg tgggcgagaa cgtcgaatac gggctcaagg tgcgcggcgt gaagccggcc
    29821 gagcggcgga cgcgtgcccg cgaggcgctg cgcctggtgc ggctcgagga gaagcacgac
    29881 cggcgtatcc accagctcag cggtggcgag cgccagcgcg tggcgctggc ccgcgccctg
    29941 gcgccgcggc cggccatcct gctgctcgac gagcccatcg gcgcgctcga cgagaagctg
    30001 cggctcgaca tgcaggtcga gctcctcgag atccacaagc ggctcggcat gactttcgtc
    30061 tacatcacgc acagccagga agaggcgctg accatgagcg accgcatcat cctgatgcgc
    30121 aagggccgga tcgagcagtc cggcccgccg acggacatct tcgatcggcc ggtcaaccgt
    30181 tttgtcgccg cgttcatggg cgtcgagaac ctgctcgaag cgacccttct gacgctcgac
    30241 ggcgatcatg cgacgttcag gctgggcgaa gccacgttca gcggcagatg gtcagggacc
    30301 ggatcgccag agatcggcag cgacatcgtg gtcggcctgc gcgccgagcg gctgcatgtg
    30361 gccgatgcgg cacccgaatc ccctgccgcc gcaaacgtcc tgccctgccg gtttggcgct
    30421 gccatctaca agggcaagta tctcgaccgc acggcgaata ccgctatcgg acagttgaag
    30481 gtccgcatct gggacgccaa cccggtgacc cgggacgacg ccttcatctg gtggaagcgc
    30541 gaggactgca tggtgatgag cccttgagcc gatccggcgg cagcgcgccg gccgagttgg
    30601 cgctgcatcc ctctccgtcg accaacgagc caacgagtaa aagggagaat cagccatgag
    30661 cagcttcgac atcagcaggc ggagcttcgt cgacaaggcg ctgacattgg gtggcctcgc
    30721 cgccgccgcc ggctcggtgc cgctcctcgg ccggcaggcg ctggcagcgg gcgagaccgt
    30781 caaggcatat ggcaccacca cggcccagct tcgagactgg tccatcatgg agaactcgat
    30841 cggcatcaag gtcgagttca caccgaccaa taccgacatc ggcatcttca tgcgggatat
    30901 catctccaac gatctggggg ctacccagga catcttcatt ttcgacggcg gcaccgagaa
    30961 cctgctcggt ccgcagggct actatgccga gatcatcgag gaccagccgg agctgacgct
    31021 ctgggagcgc acgccggacg cctggaagcg ggccgactgg ctgcgtttcg acggcaagca
    31081 gtacggggtg ccggtcatcg gcaacggcga cgccttcggc tatttccctg aggtcatcga
    31141 tgccaatccc gacggcatgg acgagatccc ctggacgacg ttcttcgagg acgagcgggt
    31201 caagggccgt gtcgccatcg accgcagctg gctgcagtcg ttgcccgaga cggccaactt
    31261 cctcaagcac cacgggcggg tggccatcga ggatccggcc gacctcacgc ccgaggaggc
    31321 gcggccggtc gtcgactttc tgatcgagcg caagcgtgcc ggccacttcc gcacgatctt
    31381 caccgccttc gaggagcaga tccagctcct gtccaaccgc gaggtggaca tgatcaattg
    31441 ctgggagcct gcggtcaagg aggccaacga ggcgctcggc cccggcaccg tgatctacgc
    31501 tttcacggtg gagggctact acaagtgggg ccacagcgcc aatgtctcga gccaggcgat
    31561 ggagcgcggc aacctcgaca acatctacaa gctgctcaac tacttcctcg gtggcgagta
    31621 ccgcgcgtac caggccaagc agcgcggcta cgcggggccc aacatggatc tcggcgtcgc
    31681 ctatgccacc gagcatggct ggccgcagga ggatatcgac ctcctgaagt ggaccgagga
    31741 gaaggtcgcc cgcaagttcg agaagccgtt ctgggcgaat acgacgccgg tcaatgccga
    31801 tgtcatggaa agcgagtggc agcgcttcct caacgcctga ccccctgata gggcgggaac
    31861 gtggacgggc ggagccgggg cttcgcccgt tcacggcccg gaccggagcc gagccatggc
    31921 gacagccgac ctgacccgaa aagaaacggc gctcgatcgg ctggccgcgc gcctgtcgcc
    31981 ggccgacctc gccgtgccga ccttcctcgt tgtctggtcg gccgtcgtca tcctgccgct
    32041 cctgatcctc gtcgtcttca gcttctttca ggtgcgctcg tatcagatcg tctacgagcc
    32101 gaccttcgcc acctggcagt cggtcgtcga gaccggccgc tggaccgtgg cgctgcgcac
    32161 gctccgggtg gcgctgacga tgaccgtgat cgagctcctt ttagcctttc ccttcgccct
    32221 ctggctcgcc aagggcctgc ggtcgaagac gatcaaggcg atcatcatca ccttcctgac
    32281 gatccccttc ttcctcgacg tttcgtccag gatcctcgtc tggcgctcga ttctcggcac
    32341 caacggcatc atcaacaccg ttctgacctc gaccggcctg gtcgacgagc cgatcagctg
    32401 gctgctctac tccgagttcg ccgtgcattt cggcatgctc ggcgccaact ttcctaccat
    32461 ggtcttcccg atcttcatca tcctcgcgct gatcgacgac gactatatcc atgccagcgg
    32521 cgatctcggg gcgagccccg tgcagaccct gctgcacgtc atcatcccga tggcgctgcc
    32581 cggcatcctc gccggcatca tcttcacctt cgtgccgttg atggcggcct tcgtggagcc
    32641 gcagcttctg ggcggcggct tcgtcgacct gctcggcaac tcggtcgatt ccgccctgca
    32701 gcagatccgc tatccgatgg cggcggccct ctcgaccgtg gtcgtcgtgc tgctcggact
    32761 ctgcctcggc gccttcatgc tgctcacccg caaccgcctc gatctcgcca ccatcttctc
    32821 ggcgctccac cgatgagcac cagcaccctg ccggtggccc tggtcgacga gagcgagatc
    32881 gccccgacca tccgccttct ccgtcaggcc agccgctggc gggtctggcc cggtgtcgcc
    32941 atggcgctgg gcttcttcgc catcgccgcg atctacatcc cgctcgtctg gctcgccgtc
    33001 atgtccttct cggcacggcc gctctccggc atcccctatc cgttgacgct cgccaacttc
    33061 agcctgctgc tgggcgacga acgctgggtg ccgccgctgt ggacgagcat ctggatgagc
    33121 gcgctcgtgg cgctctgctg catgatcgtg gcgacgttcg tagggcgcgc catcacccgc
    33181 atgcgccggc ccggacccgt gctgctcttg acgctgctgc cgctcttcgt cccggggctc
    33241 acaatgggtg ccgccctctt catcttcctg cggatcctga tggggctgac gctcggcctc
    33301 tggtcgatct tcatcgccca gctcgtctgg gccctgccgt tcgcgctgct gctggtgctc
    33361 gtcgtcgcca gccgcttcga cattcgcctc ctcgaggccg ccgaggatct cggcgcctcg
    33421 ccctggcggc ggttctggga aatcgagatg ccgctgctcc gcccgggcgt gctcggcgcc
    33481 ggcgtcttcg gctttctgct ctctttcaac gagctgctgc gctcgctctt cgtccgtggc
    33541 atcgagacca ccatgccgat ctacaactgg gcgatggcgg cctcgcagca gtcgcaggtg
    33601 ccgatcatct tcgcgctggc gacgatcatt ctcttcgtca ccctgccggt catgggtgcg
    33661 ttcttctggt tcctgttcgt gaagctcgat cgcgactgac gggctgcatg ggacggggaa
    33721 gtgtcgcctg cgaaacgggc caccggcgct gccggccaag gccaacaata gagggtcaga
    33781 tatgctcgat gaacgtccca acttctcgct cgaggacatg gatcgcgaga gcctgttcca
    33841 tccgctgacc tcgatcgccg cccatcagaa gaacggtccc ctgatcatgg accggggcca
    33901 gggcgccatg ctgcgcgatc acggcgggaa cgagatcgtc gactgctcgg gtggtctctg
    33961 gtgcgtgaat gtcggctatg gtcggcccga gctcgccgag gcggcgaagc aggccatgct
    34021 ggatctcaac tactggcacc tcttcggctc gtcgtcgcac gagtccgcca tccgcctcgc
    34081 cgaccggatc ctgcagctgc tgcacgagca ggccggcgcc cggcacctgt cgaaggtctt
    34141 cttcggcacg tccggctcgg atgccaacga cacgaactac aagctcgtcc gctactgggc
    34201 gacgctccgg ggcacgcccg agaagaagaa gatcatctcg cgcaccggcg gctaccacgg
    34261 gctgaccacg attgcatcgg cgctcaccgg catcgaggcc taccacaagg cgtggaacct
    34321 gccggaggtc gacgtccgct atacgagctg cccgcaccac taccgtttcg cggaggatgg
    34381 cgagagcgag gccgcgttct gcgaccggct gatcgccgag ctcgaggcga tgatcgagcg
    34441 ggaggggccg gagaccatcg gcggcttcat cgccgagccg atcatgggga cgggcggcgt
    34501 cctgatcccg ccggcgggct atttcgagcg ggtgcaggcc gtgctgaacc ggcacgacat
    34561 cctcttcatc gccgacgagg tgatctccgg cttctgccgc accggcgagt ggttcgccac
    34621 cgggctctac gatctgaagc ccgatcttct gacgctcgcc aagggtgtca ccagcgccta
    34681 cttcccggtt tccgcctcgg tcgtctcgga acgggtctgg aaggtgctgg aggcgggctc
    34741 gccgaagcac ggtgcggtga tgcacggctt cacctattcg ggtcatccgg tgggctgcgc
    34801 ggtcggcctc gccaacctcg agatcctcga gcgcgagagc atggcggcga acgcggccgc
    34861 ggtcggcggc catctgctcg ccgggctgaa ggaccggctg ggcgagcatc cgttcatcgg
    34921 cgatattcgc ggcgaggggc tgatgatcgg cgtcgagttc accgccgacc gggccaggcg
    34981 ccggccgttc aaggccgaga ccgtgccgca ccgcctggtg gccgccaagg cgctcgagca
    35041 cgggctcatg gtccgcggcc tgccgttcat cgaggtgatc tccttctcgc caccgctctg
    35101 catcacgaaa gctgaatgtg atcaggccgt cgatcgcttc gccgccgccg tcgacgagat
    35161 gacgccggag ctcacccggc tcgctgcggg ctgagtggcc gcccgcgagc cggcggatgg
    35221 cgggagtgcc ggggccgcca gcgcccctcg cgagggggca cctcctcgcg agggccaccc
    35281 gccgaccgaa gggcccgagg cgcggaccct ggcgcagcgg gtctacgaga ccctggaaaa
    35341 gcgcattctc gacggcgagt ggctgcccga gagcaaggtc agcctgcgca cgctggcgat
    35401 ctcgctcgat accagcatgc agccggtgcg cgaggccgtc ggccggctga tcgacgcggc
    35461 tgccctgcgc tcggcgcccg gcaaggcgat ccgggtgccc aggctcgacc gaaggacggc
    35521 ggacgagatc tggtcgctgc gcttcctgct cgagggcgag gccgccgccc ggttcgccgc
    35581 ccgcaagcga ccggccgagg cgcgtcagct ctacacctat accaaggcgc tcagggccat
    35641 gcgcttcggc gcggacctgc agccgacgat gcggcgcatg atggcctgga acctggcgct
    35701 ggccacgggc tccggctcac ccatcctgat cgacatcatc cgccggctgc gcctgcgcta
    35761 cgcgcccttc gtcgccgagg cgctggccga ggaggcgcct ttcgatctcg agttccacca
    35821 gttcaccctg cacatgcagg acgagctggt gatggcgatc gaaggaggcg acgccgacgc
    35881 cgcccgccat ctgcgcggcg ccgacctccg ctcgttccag cgctacctct acgcccgcaa
    35941 atcatggcgc ccgatcgctt gagcgtggcg tccgcctgcg gcaagctcct ttcgcaggcc
    36001 ggcgggtccg gcccggacag gagcgccatg caggtctttt tccacgaggc gcagcggcag
    36061 cacgatccgc agcatttcct cgtggccggc acgttgcggc cgagccccga gcagccggcg
    36121 cggatcgacg cgctgctggc agcggcgcgg aacctggggc tggaggttca gtcacccggc
    36181 gatgcgggca tggcacccat cgccgccgtg cactcgaccg actacctctc tttcctccag
    36241 acgatccacg agcgctggtc cgctttgtcg ggcgctggtc cggaggtggt gcccaacatc
    36301 caccccgagg gccgaagcct cgtctatccg gcgcatccgg cggggctcgc cggctatcac
    36361 caggcggaca cggcctgccc gatcggcccg cacacttggc actccgccta ctggagcgcc
    36421 cagacggcga tcgccgccgc cgacgcggtg ctgcgcgggg ccgcctcggc ctatgcgctc
    36481 tgccgcccgc cagggcacca tgcgacgagc gaccgggccg gcggcttctg cttcttgagc
    36541 aacgccgcca tcgccgccga gcgctgcctg cgggccggca ggcgaccggc cgtgctcgac
    36601 atcgacctgc accacggcaa tggcacgcag caggtgttct acgagcggca agacgtgctg
    36661 acggtctcga tccacgccga tccgagccgg ttctacccgt tcttctgggg tcacgcgggc
    36721 gagcgcggcg ccggccccgg ccatggctac aacctcaacc tgccgctcgc gctcggcacg
    36781 ggcgacgacg gctttctcgc ggcgctggcc gaggcgagcg agcgcatccg cctcttcggc
    36841 gccgatctct tggtccttgc tgtcggtctc gacgcgttcg agggtgatcc gttcgccggc
    36901 ctggcggtga cgaccggggg cttcgggcgg atcgccacag cctgcgcctc gctcggcctg
    36961 ccgaccgtgc tggtccagga gggcggctat ctcagccccg agctcacccg aaacctcgaa
    37021 tcctttctgg cctcgtggct gcgtccggcc ccgtccggcc gctgaccaac cgggatccga
    37081 cgcatgcgcc ttctcctgat caacggcaac accacgcagt tcgtcaccga tgccgcggcc
    37141 agggaggcac ggctggcggc cgcagatggc accgaggtcg tggcggtgac cggccggtcg
    37201 ggcgccagga tcatcggcac gaggttcgag aacgccctgg cgcagcacga gatgatcgcc
    37261 ctcgcggcgg cgcatggcga gggcatcgac gcggtgttga tcgccgtctc ctacgacacc
    37321 gcgctcccgg ccctgcgcga gcttctgtcc gtgccggtgg tcggcatgac cgaggcggcg
    37381 ctgctcacgg cccacctcgc cggcggccgg atcggcatgg tctcgctggg caggcgggtg
    37441 cagccgctct atcgcgaatt ggcagcggcc tacggcctcg accggcgcat cgccggctgg
    37501 cgcacggtcg agagcacgaa ggccttcgcc ccgggcgaca cggccgagct ggatgggctt
    37561 ttggccgggg cttgcaacga gctggtggag caggagctgg cggaggtcgt cgtgctgctg
    37621 ggcgcggtga tggcgggagc accccggcgt ctccaggcgc gagtgccggt gccgctggtc
    37681 gagggcatcg ccagcggcgt cggccaggcc gagcttctgg tccgcctcgg cctgccgaag
    37741 cccggggccg gcagctacgc gctgcccggc cggcgcgagc tcgccggcgt cgagccggcg
    37801 atcctggccc tgctgcggcg ccagcccggc tagacggcgc tggcgtaggg gccgaacatg
    37861 cgggtcgagc cgtcggcgta gccgaccatc atcgagtcgg cgacggtcga gaagaggcca
    37921 ttctcgacca cgcccggaac ccggttcagg gccgcttcca gcgccggcgg gtcgggaatg
    37981 gcgccgagcc ggcagtcgag caggaaatgg ccgctgtcgg tcaggagcgg gccgccttcc
    38041 ccgccgcgca gggtgatcct cggctcgacg tgggcgtgct cggcgaggat ctgaccgatc
    38101 tgccgcctcg tcgcttgcca gccgaagcgg acgacctcga ccggcagggg aaaagcgccc
    38161 agcgtctcca cgaccttcgt ctcgtcgacc agcgccacca tgcgcctcga cgccatcgcc
    38221 acgatcttct cccagagcag gcaggcgccg ccgcccttga tcatgcgaaa gcggtggtcg
    38281 atcccgtccg ccccgtcgag cgtgagatcg agctcgccgg cctcgtcgag ctcgatcacc
    38341 cgcacgccga cctcgcgggc gagggcggcg gtcccggtcg aggtggccac acccacgacg
    38401 tcgagcccgg cccgaacccg ctcccccaag gcccggacca tccactccga ggtcgagccc
    38461 gagccgagcc cgagcttcat gcccggcgcc acgaggcgct cgacggcagc cgccgcgacg
    38521 gcacgcttgg cgatgtcctg tggtgtcatg ccgacatcct cgatcttccg cgatggacag
    38581 tgtcatatcg gactgacggc tgacaagtcg ccccgaacgt tggccggcag tgctgttgca
    38641 tccactcctg gtgacctgga aacgacccga tgacgcaaac gactctgcgg gacgatgagc
    38701 tgctcgacct cgtgcaacgc cagacgctgc gctatttctg ggacttcgcc cacccggtga
    38761 gcggcctcgc cagggagcgg agcgacgtca cgccgggctg cgggccggac gttgtcacga
    38821 ccggcggcac gggcttcggc atcatggcca tcatcgcggg cgtggagcgc ggctggctcg
    38881 accgtgccgc ggcgtcgaac cgcctcctcg aaatggcccg tttcctcggg catgccgagc
    38941 gtcatcacgg catcttcgcg cactggctcg acggccgcac ggggcgcacg gtggcgttca
    39001 gcgagctcga cgacggggcg gatctcgtcg agacgtcgtt cctgctcatg ggcctgctca
    39061 cggcccggca gtatttcggc ggcaacggcg ctgcggaacg ggagctgcgg gagcgcgtcg
    39121 acgccctctg gaatgaagcc gagttcgact ggcacacgaa gggcgacgag gacatgctct
    39181 actggcactg gagcgagcgc cacggctggg cgatgaacca cccgatccgg ggctggaacg
    39241 aatgcctcgt cacctacgtg ctggccgcat cgtcgcggcg ccacgccgtc cgccccgagg
    39301 cctatcaccg gggctgggca ggcgggtcgc gcttcgccaa tggccgcacg tatcacggca
    39361 tccgcctgcc gctcggcccg gaccatggcg gccctctatt cttcgcccac tacagcttca
    39421 tgggcctcga cccgcgcggc ctcgaggatc gctacgccga ctacctcgag cagaacgtgg
    39481 cgcacacgct gatcaaccac cgccactgcg tcgccaaccc gaacggccat gccggctatg
    39541 gcgaggcctg ctgggggctg acggcgagcg acgatccctg gggctacgag gcgcacgcgc
    39601 cggaccggga ccggggcgtc atcacgccca cggcagccat cgcctcgatg ccgtacacgc
    39661 ccgcgctgag cctcgccgcc cttcgccact tcatggacta tgacgatggc cggctctgga
    39721 cggacatggg cttcgtcgat gccttctgcc ccgccgaggc atggcgcgcc ggctcgcacc
    39781 tcgccatcga ccaggggccg atcgtggtga tgatcgagaa ccaccgcagc gggctgctct
    39841 ggcggctgtt catgagctgc ccggagatcc gggacgggct gcggcggctg gggttcacga
    39901 gcccgcattt gggcgcgacc tcggcctgat gcgccgatgc tcgcgctacg cccgcgcggt
    39961 tgccgcccgc agccgctcca gcaagggtgc gaagccgatc ggcacgacgc ccgaatcctc
    40021 gacgtggcgg aggaaacccg gatcacggcc gagccggtgc tcgtcggtcc ggcaatgggc
    40081 gagatccggc ttgatcacct cgatgtcgcc gggcgcattg ggatgcagcg agaagaaggt
    40141 gacccccggc cgcagagcgt cgatcatctg ccgataggtg gcgtcgctca gggcgctggc
    40201 cacccagggc gtcatgacga agcggtcgac gaaggcggca ccccgggcgg caagggcggc
    40261 atgacgctcg gtgtagaagg cgggatcgac cgggccgagg ttcaggacgc ccagatagct
    40321 ctcgatctcg cgggggaaga gcagcggcag ccggtattca tcggcgaggc cgatcgtgat
    40381 gtcggcgagc tctggcacca gggccgcccc catatgcgtg tcgagatgcg tcggccggat
    40441 gcccatcgcc atggcgcgct cgatctgggc gcgcatctcg tccccggccg cgaggggatc
    40501 gagccgggcg cgcagcggcg gcacccggct ggggaaatag ccgtcggcat cgacg
//
LOCUS       DAOTFI010000007        39113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1104_length_39113_cov_7.107686, whole genome shotgun sequence.
ACCESSION   DAOTFI010000007 DAOTFI010000000
VERSION     DAOTFI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 39113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39113
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1104_length_39113_cov_7.107686"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="P5558_01240"
     CDS             complement(<1..1012)
                     /locus_tag="P5558_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HRY22965.1"
                     /translation="MQADKVDFAALIENDRIERQRQEWQGTFLEYLELVKAQPDLADL
                     AHRRVYHMIAGRGVADLDLEADPRAKRVFGDEPVKVYDFFKDEFFGMERTLEKIVRYF
                     HAAAMGGEEARQVLYFMGPVGSGKSSLMERLKRGLEELEPIFVIDGSPNYSNPLCLIP
                     RHLRPAFEEMLGVRIEGDIDPITRHRLMTEFKGEYEKMPVRTMSFSIRGRRGVGVVPP
                     VDPNNQDTSVLIGSEDISKLDRYSEGDPRVLELSGAFNVGNRGLVEFIEVFKNETEYL
                     HTMITATQEKMVPAPGRHGTVYVDTCIVAHSNEAEWQKFKADHTNEAILDRIVVVKVP
                     YNL"
     gene            complement(1394..2725)
                     /locus_tag="P5558_01245"
     CDS             complement(1394..2725)
                     /locus_tag="P5558_01245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRY22966.1"
                     /translation="MKAWRAVLVLVLVLVGGTARPLLAQGLIRDAEVEAELRLIAEPI
                     FAAAGLDPESVRIYMINDPTLNAFVAGGQNLFIHTGLILRTETPDQLAGVIAHETGHI
                     AGGHLSRSRDAMGQAQTEAIIGALLGAAAIVAGAPQVGTAIIAGGATVAERNLLKFSR
                     GQEQAADQAAIKYLAAEELPPTGLAEFFRVLENQNLRINSEGPSYLRTHPLTRDRITF
                     VEGEAARSPYAGRTYPPEVEEAHGRVRAKLEGFLLEPMEVIQRNTGDTYNERYARAVA
                     LFRRGETDRALELVDGLIVERPEDPYLHELRGQILFESGRVAAAVEPYRVALAGRPDS
                     ALIRFGLGRALLESPEPEAVNEAAVLLREVVRLEPENAYAWRFLGVAEGRLGNQGRAS
                     LALAENAVLLRKPEDAELYLRRARQHVQPNDPDWVHLQDLELAAGRLGDAG"
     gene            complement(2853..5462)
                     /locus_tag="P5558_01250"
     CDS             complement(2853..5462)
                     /locus_tag="P5558_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRY22967.1"
                     /translation="MTKRMLIDATHAEETRVVVLQDQQLVDFDFESSTKKQLKGNVYL
                     AKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYFQIPQADRELLAKLERESVEADAA
                     NGDARRGRGRKRRAEAAEDESDAAEGDAAEGDAAYGEARDGEADDDERPIRVREDSEE
                     ETADVMSEQARRRAKLLRSYKIQEVIKRRQIMLVQVVKEERGNKGAALTTYLSLAGRY
                     CVLMPNTARGGGISRKISSSEDRSKLKAIVAELDVPQGMGLIVRTAGQQRSRAEIKRD
                     YDYLMRLWSDIRDTTLQSVAPALIYEEGDLIKRAMRDLYSRDIDEVLVEGEEAHKSAK
                     RLMTMMMPSRARHVKLYKEELPLFFKHKVEDQLDAMHSPIVQLPSGGSIVMHATEALI
                     AIDVNSGKSTRERHIDETAVKTNLEAADEIARQLRLRDLAGLVVIDFIDMGEMRHQRA
                     VERRVRDALKIDRARIQLGKISMFGLLELSRQRLRPSLQELSSRPCPTCQGLGSVRST
                     ESCALQALRLAEEAGIRNEAKVLRLTLPGEVALYLLNQKRDGLVRVEQRYAMRVEIVV
                     DPEMTPGHHSVEVVQRRAAHEVVPPRPAMVEEPVEPATAEADDADDADEADERPVERA
                     PTGTRETAKVEDDEEGGRRRRRRRRRRRRPEENGGEASARADAAAEGEADEEPSSPAA
                     SAPDGPAEAQVSDTVVVPAEAASPDGVEAASPEAVDAAAPPAEPRKPRRRRRTRRPTD
                     EAAATEAPSADDVDDAPAASAEAVAGDDAAPVAIADDPEPAPSEPAPSEPLPVEPLPG
                     QPAVEVPEPVAAEAAPEPAYEPAPEAIPEPAPEPLDAPTAQPLREEREMALADEAPEP
                     QPARQGWWSRWVR"
     gene            5528..5791
                     /locus_tag="P5558_01255"
     CDS             5528..5791
                     /locus_tag="P5558_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22968.1"
                     /translation="MAHHRAGAVGGLVVMRRVATVTRHAEACRTVATRRAMAVSSFGA
                     TFASRPAGRPPLLAASWSNAGRSLVPTGIPPVDPAMSLRRLDR"
     gene            6000..7325
                     /locus_tag="P5558_01260"
     CDS             6000..7325
                     /locus_tag="P5558_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY22969.1"
                     /translation="MLATGLALFATVVLLLFGRALASETPEVVGVRFGINEAVTRVVL
                     DVDRPVRFGATVLADPPRLVVDLDDVRWRVREHPQNRPVGLAQSVTYGRLGPERARLV
                     VDVGRPFRIVRQEQLAPSAEVRHHRILIDITALPPPAPAAVAAAPATARPAAATARAP
                     QFARPLPRPVRVARATADPALGAIPAPPSERPEDVTLAAVPAAPLRPADPPLRRRVVV
                     IDAGHGGIDPGTIGINGVPEKVITRQIADRLTELIEGTGRYDVVLTRRGDEFVALRDR
                     IQRAHGANASLFISIHADSLADARLRGASVYTLSDTASDSEAALLAAKENKADIIAGT
                     DLSAHDEVVAGILIDLAQRDTNNKSIEFADVLTAELGEVTRLLRNTRRFAGFVVLKSP
                     DTPSVLLELGYLSNPTDAANLADPAYQKRLAEATLRAIDRYFGVTQARS"
     gene            7509..9971
                     /locus_tag="P5558_01265"
     CDS             7509..9971
                     /locus_tag="P5558_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRY22970.1"
                     /translation="MSVLRWLVKALAWLGVTAAVWLVIAGAGVAYIVHRYAADLPDFA
                     RLADYQPPTVTRIHAADGRLLAEYAHEKRVFVPVDAIPAMVKQAFISAEDKSFYQHPG
                     IDPVGIARAAIDNFKALGTGQRPQGASTITQQVAKNFLLTNEVSLERKLKEAILALRI
                     EQAFTKDRILELYLNEIFLGRRSYGVAAAALSYFDKALDELTLAEAAFLAALPKAPST
                     YDPDRAPELARGRRDYVLDRLLADGAITAAEHAAAIAEPLVTRSRDSIDYARADFFTE
                     DVRRQLVREFGEDGFYQGGLSVRTTVDPELQAKADKALREGLATYDRERQGWRGPLGV
                     LDLAATTDWPAAVASFDPGFEVEPWRVALVLEAGSGAAGIGFADGSRTSLALADVRWA
                     TPELGDGEFGAPVDAVSDVLAPGDLVAVEWVTDGEEAGGRWALRQRPSVEGALVALDP
                     HTGRVLAMSGGFSFRQSQFNRATQAYRQPGSAFKPFVYLAALENGFTPASIILDAPLV
                     IDQGPDLPVWKPENYSDNFYGPSTLRVGVEKSRNLMTVRLAREIGMDKVVDVARRFGI
                     DRGLGTNLAASLGANEVDLMSLTEAYAMLVNGGREIEGRLVERIQDRFGRTVMRRDER
                     PCPDCRKLAWDSEPPPVLPDTRPVVEDAPRAYQMVSILEGVVQRGTGVAASRIGKPLG
                     GKTGTTNDSRDAWFVGFSPDLVVGVYVGFDQPRNLGRKTTGANVALPIWIDFMAAALE
                     DAPATPFRTPPGVRVVRIDADTGLLPGPATTSVIAEAFLPGTEPTERSTGAPTAANPD
                     PNGQALPSLPRTAGGGSAGGLY"
     gene            10018..11140
                     /gene="prfB"
                     /locus_tag="P5558_01270"
     CDS             join(10018..10086,10088..11140)
                     /gene="prfB"
                     /locus_tag="P5558_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152415466.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRY22971.1"
                     /translation="MRAEIINLNDAVEQGLVLLRRHLDWDNARRRRDELDSLSEDPNL
                     WNDAEKAQALMRERQHLADAIDGQLAMEQARADALELAELAELEDDDATLDSIEAELR
                     GIKARIDKLALQALLSGEADGNNAFVQVQAGAGGTESQDWAEMLLRMYLRWAEAHGHK
                     IEWIEENAGEEAGIKSATIKVLGTNAYGWLKTESGVHRLVRISPYDSAARRHTSFASV
                     WVSPEVDDSIEIEINDKDLKVDTYRASGAGGQHVNRTDSAVRLTHLPTGIVAACQNDR
                     SQHKNRATAMNMLRAKLYDLELQKRRAEQDATEATKSDIGFGHQIRSYVLHPYKMVKD
                     LRTGTESPNPTAVLDGDLDPFLEASLAQGAGHTRVEEAQ"
     gene            complement(11213..>11583)
                     /locus_tag="P5558_01275"
     CDS             complement(11213..>11583)
                     /locus_tag="P5558_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY22972.1"
                     /translation="EECAGRFDIMSRTGAIYFRTGSAVLDQESRPLLDTVVAIVERCP
                     GVAVRVAGHTDSVGPDELNQALSEARARSVTTYLEEHGVPAARLQSIGYGETRPVAPN
                     DTPWNRSRNRRIEFLVVGEG"
     assembly_gap    11584..11683
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11684..12279)
                     /locus_tag="P5558_01280"
     CDS             complement(<11684..12279)
                     /locus_tag="P5558_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRY22973.1"
                     /translation="MISASPAAIAARGRGPAALLLAALLVLSGAGAVAQEPFAPGWTM
                     APELSKLDVQSVKNETKVESSTFAAFEGAIAPDGQATVTIQLNSIDTGVDLRNVRMRF
                     LFFETFKYPEAVVTVQLDRAMLGDLESRRRLNLTLPFELDLHGITKELETEVVLTLLT
                     DELVSVASSRPISIGTELFDLTDGLKKLEEAASVSVIPS"
     gene            12402..13718
                     /locus_tag="P5558_01285"
     CDS             12402..13718
                     /locus_tag="P5558_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase domain-containing protein"
                     /protein_id="HRY22974.1"
                     /translation="MRWLALKSGFFRCVGVVLAALAGVATLAHAGTARAEQRIALVIG
                     NAAYEINPALDNPAHDARLMAETLGSLGFEVIQLIDADQATMKDAIANFGRSLRAGGP
                     DTVGLFYYAGHAVQAKGNNYLIPIRSTIRDEADLDLVGVEGRWVLRQMESARNITNIV
                     ILDACRDNPFAEVGAVEQGLARMDAPTGTFITYATAPGRVAYDGTGMNSPFTAALAQA
                     MTQPGVPIEQMFKDVRVAVLEATNGAQTPWDSSSLVRDFTFRPAEMPTAQEQAERRLW
                     EAVSATRDPVQLVLFLRNYPDSPYADEARSLVNEALLGVSAEPVPAPGETGSGAAEVE
                     VELAAVPGALGFSLPLVSDDPSISGRSIEQIIKGSPKFTPVEGLDDAAWKGQTCSTCH
                     QWTKEALCDQGKFYVKEGEARTQSKLHPLGGAFKLALFEWAGADCP"
     gene            complement(13675..14430)
                     /locus_tag="P5558_01290"
     CDS             complement(13675..14430)
                     /locus_tag="P5558_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22975.1"
                     /translation="MPDPADILLVQLDTEAPPGLIEAVAEASGRHLTMIDARDETPLD
                     VLGSAAGLIVLGSAAVALDPGSPLVAVLRERVRQDQPVLALDTGAALLALACGGTVRA
                     GGSHEFNYVDLTPTALTEADPVLRALGSGLPFMEWHDDAVDLPDEVAILAVCGRARSQ
                     AFRVGHRVYGLRFHPGATAEIIRRWTAIRSAASNNPAIRVRIGAETVRHQERAERFGR
                     AVIEAWLALCRMDELAANDLRGSRRRPTRTAPA"
     gene            complement(14433..15155)
                     /locus_tag="P5558_01295"
     CDS             complement(14433..15155)
                     /locus_tag="P5558_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568110.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRY22976.1"
                     /translation="MQPVVRLVAAALVGAGLAAAGWFVGEGFRQGRTAERVVTVKGLA
                     EREVRADLALWPMRFVATSDDLAEAQATLARAEAAVLAFLAAGGIQRDAIEVQGIDVT
                     DLLAQAYRSGPVESRYILGENLMVRFADVDAIKALSQDVGKLVEAGVVLTSEGLRGPF
                     YLFTGLNDVKPGMIAEATANARAGAEQFARDSGSAIQGIQRASQGLFQILPRDNAPGQ
                     SEELQVTKTVRVVTTIDYRLAD"
     gene            complement(15164..17104)
                     /locus_tag="P5558_01300"
     CDS             complement(15164..17104)
                     /locus_tag="P5558_01300"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HRY22977.1"
                     /translation="MNGILWQPGAERANGTAIMTMARRQGFTGADAVEELRRWSVAEP
                     AAFWAGVWELGGVQASVPAGTVLAEADKMPGARWFEGARLNFAANLLRRDDATPALIF
                     RGEDGTRRELSWSALQAEVRALAAELAADGVGVGDRVAGYLPNCPEAIIAMLATTALG
                     AIWSSASPDFGVAGVLDRFGQIEPRVLVTVDGYHYAGKRLDIRQKVRDVAAGLPGLRR
                     TVVAPFLESDPNLGGIPRGVSWGDYCINVDKPLTFVQLPFDHPVYILYSSGTTGKPKA
                     IVHGAGGTLLQHLKEHLLHCDTRPGDRVFYFTTCGWMMWNWLVGGLAAEETLVLFDGS
                     PFHPEPAALFDLAEEEKVAVFGTSAKYIDACKKAGLRPKDTHDLGRLRAILSTGSPLV
                     PESFDYVYDAIKSDVQLASISGGTDIVSCFVLGNPAGPVHRGEIQMRGLGMAVEVWNE
                     EGRPVTGEKGELVCTAPFPCMPVGFWNDPDGSRYHDAYFARFPGVWCHGDFAELTPTG
                     GIVIHGRSDAVLNPGGVRIGTAEIYRQVEQIPEIQESICVGQDWEGDVRVVLFVRLAQ
                     GAVLNAALEQAIRSRIRANCTPRHVPARIVAVGDIPRTRSGKISEIAVRDVVHGRPVK
                     NTEALANPEALDLYRDLEALQE"
     gene            17243..18322
                     /locus_tag="P5558_01305"
     CDS             17243..18322
                     /locus_tag="P5558_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY22978.1"
                     /translation="MIRRTLLATTLFAGLAATTTLALAQDDPFKVGFVYVGPVGDHGW
                     SYQHDQGRQALEAHFGDAVETTFVENVSEGADAERVIRQLASSGHDMIFTTSFGFMNA
                     TAKVAQQFPDVIFEHATGYQQADNLATYLSTTYQGRYVSGFLAGKVSQTGKIGYIASF
                     PIPEVIRDINAVMLGLQEVRDDAELVVIWVNTWYDPAKESDTARVMIDQGVDVIIQHT
                     DSPAAMQVAEERGIWAVGQASDMSRFGPKAQLTAVIDDWSPYYIERVQAAMDGTWETH
                     DIWGGMHEGMIVMGPINAAVPAEVADEATAIAASIADGSFHPFTGPIRNQAGEVVIPE
                     GTVMSNEELAGMNWYVEGIEGSVPQ"
     gene            complement(18329..>19503)
                     /locus_tag="P5558_01310"
     CDS             complement(18329..>19503)
                     /locus_tag="P5558_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRY22979.1"
                     /translation="GIGLATFGVAAAVSVFGGPAFRLYAALIGMAAGVVLAASLGAIA
                     PDGWRHLDAEPWLALPRLGHLTWTISPDPTLLLAFGLAAFAATLKTIGAVSTAQRLND
                     ADWSRPDAGNLRRGVFGDGLASICAGLLGSTGQNSATSAVGISGATGATSRWIALTLA
                     GWLAAMACMPKLAALFELMLGMVLGGVLAFAAIYMVANGIELIGSRVLDRRRALVVGA
                     AIIAATSFRAMPELALALPVALQPFASSGLALGMVVAVVLNLVFRIGIHRRARLVVAD
                     LSQAEREIAAFLAVQAGLWGLRQELLARAVLALGHLLRCDGAAPQGHSRLTVEASYDE
                     LRLVLFLRRATTVPVLVPAERPSSPGRAMTLQVPGAELATMQRAVGSVCLRLELDA"
     assembly_gap    19504..19603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19604..19911)
                     /locus_tag="P5558_01315"
     CDS             complement(<19604..19911)
                     /locus_tag="P5558_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22980.1"
                     /translation="MTTRSSLDPGGLSLSLPDAVDTPSPSMRGARPPALIYAVDERPP
                     AAPLVLLGLQHMAIVASSLVVPVFVARAAGVDSATLAAIVFGIGLATFGVAAAVSVFG
                     G"
     gene            complement(20031..21854)
                     /locus_tag="P5558_01320"
     CDS             complement(20031..21854)
                     /locus_tag="P5558_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY22981.1"
                     /translation="MRQRRLEFERRYARPPVDIWAIVADTERGNELTEGLNAYTIEDT
                     ARPDGTVLRRGRGRLGPFDVEWEEGFGEWVEQRSFSQVRRYTKGPVDTIGMHVRLDAD
                     GGGTRVTYVFEAFWNSLLGNVIDRLGVLARVATNLIGTNDRRIAALDDPALAPVPKPH
                     PLEAASEARLREALAVIERGPFGHGLTSRLAGYLTTAARADLKRIRPLALARAWGAPE
                     TAVVELCVAAQAAGLLEMRWAILCPRCRVGKSVVTNLYEMPRGVHCESCNIDYERDFA
                     ANVELVFQPAGWLRALPAADFCMMSPAATPHVLVQHAVAAGAALALPLVIEPGAYRLR
                     TVEAGGESDVDHPGGAFPEVVASGGTVVAGPLAGPGTVLLRNASDRPLNLVIEERSWS
                     KDALTGDRVIAMPVFRELCPTQVLRPGDDVVIGQVTIMFSDLEGSTALYESIGDSAAY
                     SLIRDHFAFFATHVREHDGILVKTIGDAVMAAFAEPANAARAALAIQSRVAEFNAAHA
                     ANAITIKLGLHRGECIAVTTAGTLDYFGAAVNLAARLQGQSRGHDIVVSATLHDDPAV
                     AAVLAGLEQQKETTRLHGLDRPVTFHRLGVGRNRPATGVES"
     gene            complement(21865..22389)
                     /locus_tag="P5558_01325"
     CDS             complement(21865..22389)
                     /locus_tag="P5558_01325"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRY22982.1"
                     /translation="MLDAAALKALQAPLKSRYREDAEAARITLKASGEIDDQAIACKV
                     ETGRAIQIAGLHPATGGTGQELCSGDMLLEALVACAGVTLKAVATALGIELRSGRVTA
                     EGDLDFRGTLGVEREAPVGFEAIRLRFDVATDASQEQLDTLLKLTERYCVVYQTIAKG
                     PKLSVAMERAQGGA"
     gene            complement(22423..23205)
                     /locus_tag="P5558_01330"
     CDS             complement(22423..23205)
                     /locus_tag="P5558_01330"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY22983.1"
                     /translation="MLRFDQIEKIYPNGTHALTHLSLELDAGEIVAVIGGSGCGKSTL
                     LRLAAGLDRPSSGTVSVEDTVITTPHEAVGIVFQEPRLLPWLDVAGNIGFGLTHLPRA
                     ERHERVVRALERIGLAGYESAWPRELSGGQAQRVSIARALVARPKVLLLDEPFSALDA
                     FTRADLQHALLALWEASRPTLALVTHDVEEAILLADRVVVMQPSPGRVFKEIRIDLPR
                     PRHRLDDAFIAIERRVLHALDGSLGLARQRASTQANVAVGGW"
     gene            complement(23227..24081)
                     /locus_tag="P5558_01335"
     CDS             complement(23227..24081)
                     /locus_tag="P5558_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY22984.1"
                     /translation="MARAELTADLVGRPAPAARRARAGGGLAAILTSPLVLGLALPVL
                     LALVWEAAAALGLTSRRLMPPPSTIFATLFKLAANGDLWEHIWITTWRVAVGFVIGTI
                     AGTLVGAAAGYSGLTRRLVDPTLQGLRAVPSIAWVPLFILWLGIFEESKVALIAVGVF
                     FPVYLGVMGEILSVDRKIVEVGRVFRLSGPALVRRILLPAILPAYILALRAGLGLGWM
                     FVVAAEFMGASQGLGYLLVDGQQLGKPDQIIASILVFAVLGKLTDALLVAATRPFLRW
                     KDGYAPAG"
     gene            complement(24132..25112)
                     /locus_tag="P5558_01340"
     CDS             complement(24132..25112)
                     /locus_tag="P5558_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY22985.1"
                     /translation="MPTFARRLLLSAAAAAFLLQPWHSAGAADIDELVVDWAFYNPVS
                     LVLKNQGLIEEEFAKDDIEVRWVQSHGSNKALEFLSADAIQFGSTAGAASLVGRINGN
                     PIKSIYVYSKPEWTALVTTADSPIEKVEDLQGKSVAVTRGTDPHIFLMRALADAGLGE
                     DDVDFVLLQHADGRAALSRGDVDAWAGLDPMMASAELEEGARLFYRNADANSWGILNV
                     REEFAAEHPEIIARVLAVYEEGRKWALEHPDELAALLAEEAGIPVEVAQKQLGERTEL
                     THNRIGAEQRATILEAGLALQQAGVIEESVDVEAVLDELIEPSFGEALAD"
     gene            25298..26161
                     /locus_tag="P5558_01345"
     CDS             25298..26161
                     /locus_tag="P5558_01345"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRY22986.1"
                     /translation="MSSTEKVELDPAIRDAWHVCAVAADIAPGATAMHCLFGQRLEVA
                     RDAAGRPVVRGDGRVLPALERYGFIWTSPGTPPEDLFPLPGYDEPDRKNVVTGSVGIR
                     VSAPRAVENFLDMGHFPFVHTGILGEEPHTEVKEYAVAIDPVADEVVATECQFYQPRA
                     AVGSSGGAKTEYVYRVPHPFCAILYKTSPFDAVRMDIVALFVQPVGEEEIIGHTMMSI
                     IDDRSTATEVRHFQLTIFGQDKPILENQRPRKLPLDPRAETPIRADKSSIMYRRWLTS
                     KGVAYGVIPAP"
     gene            26178..27830
                     /locus_tag="P5558_01350"
     CDS             26178..27830
                     /locus_tag="P5558_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRY22987.1"
                     /translation="MLNDEQRRAWQPVASSTDLVARHVFAGAIWGQELALWRADDGHV
                     NAWENRCLHRGVRLTIGVNLGSELMCQYHGWRYANRTAGCTYIPAHPADAPARTICNR
                     VYPAVERHGLVWAALEGTVEGAALPELPMDGLVLRPVPVDVPPAEVEAALAAEFGRDR
                     VRWLVQPVDAGRSIIRGVLAEQPAEPLPVLRRWSARLGRLRDRLEALPRPPVAPIPPE
                     LHRVDPALAGLPEPGPAGTSLRLRVARKWPVAEDVTAFRLAPLAGELPAFQPGAHIDV
                     ALPNGLTRQYSLTNGPDDTAAWVIGVKREPAGLGGSTCLHDAVREGDVLAVSPPRNNF
                     PLRRDGLLTLFIAGGIGLTPLLAMARTLHLARLPYHFHAFARSVAHLPFVDVLDGLGD
                     AVERHIGLDPAATTAKLEAIIGTQGEKHELYVCGPTPFLDAARAIAAAKGWPDTAVHF
                     EYFKNERPRDASNAFEVALARSALTLDVAPGQSIVEAVAAHGIHIPTSCAQGACGTCR
                     VGVIEGAIDHQDVHLTPSEKARGDCLMACVSRAAAGRLVLDL"
     gene            27836..28471
                     /locus_tag="P5558_01355"
     CDS             27836..28471
                     /locus_tag="P5558_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007396221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY22988.1"
                     /translation="MLTLYDYELSGSCYKVRLMLGFLGLPHETRHVDFHPGREHKSDA
                     FLAINPLGQLPVLDDDGYVLRDANAILTYLASAHDPGGRWGFGGDAARNGRIAEWLGF
                     ASDLTGTIGAARLHDAFFHDIDADAARAGGKRLLRILDEHLWFQERQGAAWLAPGDHP
                     SIADIACFPYTALAQDADLDLADCPAIRRWHDRFKRVPGFTVMPGVFPARE"
     gene            complement(28481..28978)
                     /locus_tag="P5558_01360"
     CDS             complement(28481..28978)
                     /locus_tag="P5558_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22989.1"
                     /translation="MSTDLQEQTDQQLKNLIENHRRQGAVQADLYLAALAEFDRRKGK
                     GLEFQKSFDVIQAAARERRFVSYKELADASGTTWNRVHLTMGQHLGRLVEYAERRDWP
                     MLSAIVVNKPNVKSGKMEPDTLQGFVAAARELGHEVTDESAFLREQQERVFEWATLEA
                     ADAEA"
     gene            complement(29029..29856)
                     /locus_tag="P5558_01365"
     CDS             complement(29029..29856)
                     /locus_tag="P5558_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY22990.1"
                     /translation="MPSVRTSDGVALHYDEAGSGPPILLIPGWSQTAAQFGAQLEGLQ
                     DRYRLIALDHRGHGESEKPEHGYRIARLAKDVRDVLEALDLDEVTLLGHSMGCSVIWS
                     YLDTFGPERVSRLVLVDQMPCIMANPVWAEEERLAAGPILDHESLYPTINGLASQGGA
                     EVTRQFVGGMFTDAYDRARVDWAIEQNLKLPRRHAATLLYNHATQDWRDVIRRIELPT
                     LVIGGRKSLIDWRSQVWIQSVIAGSKLEIFEEDEGGNHFMFMENPTKFNRLVAEFVG"
     gene            complement(29946..30692)
                     /locus_tag="P5558_01370"
     CDS             complement(29946..30692)
                     /locus_tag="P5558_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY22991.1"
                     /translation="MSAVLALDGVVLARGGSRVLDGADLRLERGERLALVGANGAGKT
                     TLLRALVGLEKPTAGRVGAFGRPCADEPDFRTVRRRVGLLFQDPDDQLFCPTVQEDVA
                     FGPLNLGETPKAAFATARATLDRLGLDHLAERITHRLSGGEKRLVALAGLLAMAPEVL
                     LLDEPTNALDDANRARMLDLLAGLDATMLIVSHDRPALERLATRAVVLQGGRLEPAII
                     HRHPCPTPALHIHGLDDDHRHELETAARRA"
     gene            complement(30689..31414)
                     /gene="cbiQ"
                     /locus_tag="P5558_01375"
     CDS             complement(30689..31414)
                     /gene="cbiQ"
                     /locus_tag="P5558_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HRY22992.1"
                     /translation="MSRDLLAPLDPRTRLLGALALILAVLSLDGPAAGLLAVAAAFGL
                     ALAAGWRPAGLARRLAHVEGIMAALFLVLPFTTPGTTVLHLGPLAATSDGLLLALGLM
                     LRVTASALIVLALLATVEPIRLGQALARLRTPVRLVQLLLLVLRYGALLRAELRRMRE
                     AARARGFRGGLSIHALRSFGWLAGMTLVRALERAERVEEGMRLRLWQGALPVAAPGRL
                     GRADAAFGTGVLALALLAVIGIR"
     gene            complement(31411..31962)
                     /locus_tag="P5558_01380"
     CDS             complement(31411..31962)
                     /locus_tag="P5558_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY22993.1"
                     /translation="MRLALLLGTLLVAGHPASATAHQLKLFATVEAGTVTGYGFFVGG
                     GRAANALVTAAVGEAPVVAGLPVDADGSFAFAYEGPGPLRLALDAGEGHRAETTLAAE
                     RFAPSDTGEDQACTADADGLAELIEARVEAAVARQTRPLLEALAEAEARLRFSDVMAG
                     IGMIAGIAGAALWATARRRGSTS"
     gene            complement(31959..32579)
                     /gene="cbiM"
                     /locus_tag="P5558_01385"
     CDS             complement(31959..32579)
                     /gene="cbiM"
                     /locus_tag="P5558_01385"
                     /inference="COORDINATES: protein motif:HMM:NF004903.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HRY22994.1"
                     /translation="MAHIADGVLAWPVLLAGTAAAGIGVAIGLRQLDETRLARTAILA
                     AAFFAVSLVMIPLGPSSVHLLLATLMGLTIGSAAFPAVLVGLVLQLVLAGVGGLTTLG
                     INTLDIALPGVLVGALLRNFVARAAPGPAMLAAGLGAALAVALTTLGVALALTLSGEG
                     LAGAAPLVGFANLPLMLVEGVVTAFAVGFLKKVRPELLAPSAALAA"
     gene            complement(32588..33355)
                     /locus_tag="P5558_01390"
     CDS             complement(32588..33355)
                     /locus_tag="P5558_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HRY22995.1"
                     /translation="MSFRLGLVGVLWLVASPAAAHFQTILPSADVLPEGGTVTIDLVF
                     THPAFDGPAMAMAAPRRFGMVRGGETVDLLASLEPFTVDGASAWRAEVALPEPGAAVL
                     FVEPEPYFEPAEGRHIVHYAKVVVDSWASGEGWDEQLGLPVEIEPLARPTGLWTGNLF
                     RGVVRAHGEPVPYAEIEVAFVNDGRIALPNDAFGSQVIKADAQGVFAYAMPFAGWWGF
                     AALTEADETLPAPDGTPATVELGGLIWVRATDVVRAD"
     gene            33541..34545
                     /locus_tag="P5558_01395"
     CDS             33541..34545
                     /locus_tag="P5558_01395"
                     /inference="COORDINATES: protein motif:HMM:NF040065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY22996.1"
                     /translation="MADPSPLRIGMIGHGMMGVWHSQNLDDLAAVKGTIVGRRQEPTE
                     AFAAEHGYARATTDLDSMLADPAIDAVVVASPSEAHVEHCLAVLAAGKHVLLEIPIAL
                     DLAGAERVAAAAAASDRVVAVCHPWRHRPEFIALRERLAAGEEKLGMIEGRFLIHRLE
                     NVGATGYRRSWTDNILWHHGAHLIDLACWLMGEVDEVTGYMAPVDPRTGIPMTTAINM
                     AQKDGTSAHLLLTYLSQLKHNEAMALTDRDSYRIDVPRHELLTASGTLKIDAEPDLCR
                     RVIEDFVDACRHGRRPRATPADVMPAMRAMQVVQDAWDARHGVQSLPGRDLARLGQGR
                     "
     gene            complement(34533..34925)
                     /locus_tag="P5558_01400"
     CDS             complement(34533..34925)
                     /locus_tag="P5558_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY22997.1"
                     /translation="MSEKATVTASLFPATTYADAPHAVRWLQDAFGFAEKAVYPTEDG
                     KIAHAELSFGNGILMVGSARDEPGNPWAANPIGIYVVVDDVDAHCARARAAGAEIVIE
                     PRDTDYGSREYSARDCEGRLWSFGTYRP"
     gene            complement(34979..36271)
                     /locus_tag="P5558_01405"
     CDS             complement(34979..36271)
                     /locus_tag="P5558_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY22998.1"
                     /translation="MGGAVGPIILIGGLFLMILINVPVAYALGLASLATALYIGIPAE
                     AMMLKVSDGVDNFALLAIPFFVLAGAIMAQGGIAVRLVNLANVFVGFIRGGLAMVNIL
                     ASMFFGGISGSSVADTSSIGSIMIPMMKRQGYDDAFSVNVTITSSTQGIIIPPSHNAI
                     IYAYAAGGTVSIAQLFLAGIVPGVMVGVALMILTYILSVRRGYPKGELVPLGEAVRTM
                     AGALLGLFTVVIIVGGVIGGIFTATESAAIAVIYAFLVAAFAYRELRWREMPGMMVEV
                     TKTVAMVMILIGFASSFAYLMTLMQIPAKVTGFFLTISDNRIVILLLINLMLLVLGMF
                     MDMAPLILICTPILLPVMKAIGVDPVHFGIVMLLNLGIGLVTPPVGTTLFVGCAIGKV
                     RVDEVARALWPFWLTMFIVLMLVTYIPALSLAIPQMFY"
     gene            complement(36271..36777)
                     /locus_tag="P5558_01410"
     CDS             complement(36271..36777)
                     /locus_tag="P5558_01410"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY22999.1"
                     /translation="MPSTKHAYLRAMELLQALCMGFAALAIIGMTLIIAWSVYTRYWL
                     NLGSFWAEPIAIMLAVQMTFFGAAACYRVGGHISIDVVTRLLPDNGMHRAAERLVDLL
                     VMVLGLAMIWYGTGLVRTTWFQVYPEFQQIRVGLVYTAVPLSGLVTLLFAGERLVLGA
                     PPSAGEAV"
     gene            complement(36869..37837)
                     /locus_tag="P5558_01415"
     CDS             complement(36869..37837)
                     /locus_tag="P5558_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY23000.1"
                     /translation="MRLALQGTVAAVAVTFAAATSAEALVIKSSDVHAMGYPTTDAIA
                     AMGECLEEKTNGDMSIEIFHSMQLGGEKEALEQVQLGALEMTRVSVGVVGPIVDDFNA
                     FNLPYVFRSIDHSHEVVDGEIGQDLLARLEAGGLIGLGFMDAGARSFYNKERPIEKPE
                     DLQGLKVRVMQNPIFIDMMTAMGGNGIPIAFNELYTALQTGVVDGAENNPPSYLTQNH
                     YEQAGYYSLTEHLIVPEIFVFSKNVWETLDEAQQQAIRECSKVATEKERELWKAMEDQ
                     AMAKLQEMGVQINEVDKQPFIEATAPVREKFGEQYAEILGKIEAVQ"
     gene            38039..>39113
                     /locus_tag="P5558_01420"
     CDS             38039..>39113
                     /locus_tag="P5558_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY23001.1"
                     /translation="MSRARTSPRQLLAWAVYDWASSPYFAVILTFVFAAYFTQGVAAN
                     PIDGTAQWGLAMSISALLIACLSPVLGAVADAGGRRKPWLALCTGVAAVAIGLLWLVR
                     PEPGFVLLALVLMVIANTVLELGQAFYNAMLADLADPTQLGRWSGWGWGLGYLAGIAA
                     LGLVLVLFIQPDPPPFGLDAATAEPVRIVGPLIGLWLVVFALPLFLFTPDTPTRGIGF
                     GASVRAGLGRLLETLRALRQGSQVTRFLVARLIYNDGLNTLFAFGGIYAAGTFGMATD
                     EIILFAIALNVTAGIGSFAFGFLDDRLGSKRTILLALAGLVVFGTAALLAPDKATFWT
                     VGVMVGIFVGPAQSASRTLMARLS"
BASE COUNT         5982 a        13443 c        13698 g         5790 t
ORIGIN      
        1 ggaggttgta gggcaccttg accacgacga tccggtcgag gatcgcctcg ttggtatggt
       61 cggccttgaa cttctgccat tcggcctcgt tggagtgggc gacgatgcag gtgtcgacat
      121 agaccgtgcc gtggcggccc ggtgcgggca ccatcttctc ctgcgtcgcg gtgatcatcg
      181 tgtgcaggta ctcggtctcg ttcttgaaga cctcgatgaa ctcgacgagc ccgcgattgc
      241 cgacgttgaa ggcgccggaa agctccagca cgcggggatc gccctcggag tagcggtcga
      301 gcttggagat gtcctccgag ccgatcagca cggaggtgtc ctggttgttc gggtcgaccg
      361 gcggcaccac gccgaccccg cgcctcccgc gaatcgagaa gctcatggtg cggacgggca
      421 tcttctcgta ctcgcccttg aactcggtca tcaggcgatg gcgggtgatc gggtcgatgt
      481 cgccctcgat cctgacgccg agcatctcct cgaaggccgg ccgcaggtgg cgcgggatca
      541 ggcagagcgg gttgctgtag ttgggcgagc cgtcgatgac gaagatcggc tcgagctcct
      601 cgagcccccg cttcaggcgc tccatcaggc tgctcttgcc cgacccgacg ggacccatga
      661 agtacagcac ctggcgggct tcctcgccgc ccatggcggc ggcatggaag tagcggacga
      721 tcttctcgag cgtccgctcc atgccgaaga actcgtcctt gaagaaatcg tagaccttga
      781 cgggctcgtc gccgaagacg cgcttggcgc gcgggtccgc ctcgaggtcg aggtcggcga
      841 ccccccggcc ggcgatcatg tggtagacgc ggcgatgagc gaggtcggcg aggtcgggct
      901 gcgccttcac cagctcgaga tattcgagaa aagtaccttg ccattcctgg cgctgacgtt
      961 cgatccggtc gttctcgatc agagcggcaa aatcgacctt atcagcctgc atccacaaca
     1021 ccccttgccg gttgcacgtg ctctcgcgcc ctccctcatc tcgcgagatt cgactatgga
     1081 caaggtaaca cgaccgcccg acgaataaaa ttaaacttcg gcatcctctt tcaaaaaggc
     1141 ggttttttcc acggatcgaa atacccgaag aaagacgcca aatcgattat tacacacaaa
     1201 gcagcaagac acccgtccgc gcgaacgtca cgcccgtgcc ggaagcggcg gatccccaaa
     1261 attcggatgc cacatccgag tttagggtgc gccggggctc ggtcaatagg cgagccgaaa
     1321 acaacctcga gtttttttca gatgtacgaa ttcccgcatc aggcacccgc tcggcgcgtg
     1381 ccgcggcaag gtgtcagccg gcgtcgccca gccgaccggc ggcgagctcc agatcctgga
     1441 gatgcaccca atccgggtcg tttggctgga catgctgacg ggcccggcgg agatagagct
     1501 cggcatcctc cggcttgcgc agcagcacgg cgttctcggc cagcgccagc gaggcacggc
     1561 cctggttgcc gagccgccct tcggcgacgc ccaggaaccg ccaagcgtag gcgttttccg
     1621 gctccaggcg cacgacctca cgcaagagga cggcagcctc gttcaccgcc tcgggctcgg
     1681 gcgattcgag cagcgcccga cccaggccga aacggatcag cgccgaatcc gggcgcccgg
     1741 ccaaggccac gcggtacggc tcgaccgctg cggccacccg cccgctctcg aacaggatct
     1801 ggccacgcag ctcgtggaga taggggtcct ccgggcgctc cacgataagg ccgtcgacca
     1861 gctccagcgc ccgatcggtt tcgccacggc gaaatagagc gactgcccgg gcgtaacgct
     1921 cgttgtaggt atcgccggtg ttccgctgga tgacctccat cggctcgagc aggaagccct
     1981 cgagcttggc ccgaacccgg ccgtgcgcct cttcgacctc cggcgggtag gttcggccgg
     2041 catagggcga ccgggcggcc tcgccctcga cgaaggtgat ccgatcgcgg gtcagggggt
     2101 gggtgcgcag ataggacggg ccttcggaat tgatccgcag gttctggttc tcgaggacgc
     2161 ggaagaactc ggcgagcccc gtcggcggca gttcctccgc cgccagatac ttgatcgccg
     2221 cctgatcagc ggcctgctcc tggccgcggc tgaatttcag gaggttgcgc tcggcgacgg
     2281 tggcgccgcc ggcaatgatg gcggtgccga cctgtggtgc tccggcgacg atggccgcgg
     2341 caccgagcaa ggcaccgatg atggcctcgg tctgggcctg gcccatggcg tcgcgggacc
     2401 gcgacagatg cccgccggcg atgtgaccgg tttcatgggc gatcacgccg gcgagctggt
     2461 ccggcgtctc ggtgcgcagg atgagcccgg tatggatgaa gaggttctgc ccgccggcga
     2521 cgaaggcgtt gagcgtcggg tcgttgatca tgtagatccg cacgctctcc gggtcgagcc
     2581 cggcagcagc gaagatcggc tcggcgatca gccggagctc cgcctccacc tcggcgtcgc
     2641 ggatcaggcc ctgcgccagc agcggccgcg ccgtgccccc gaccagcacc aggaccagaa
     2701 ccaggaccgc gcgccaagcc ttcacccgca acctccaaag ctcgccggcc ccggcaaccg
     2761 ggccagggca cctctcatcc tgtcgagcct agcagatccg cagtgaaggc ctggtgaaga
     2821 tgcgcgacgc cgcccgcggc aggacgggcg cctcagcgga cccaccgact ccaccatccc
     2881 tgccgggccg gctgcggctc gggcgcctcg tcggccagcg ccatctcgcg ttcctcccgc
     2941 agaggctgcg ccgtcggcgc atccagcggt tccggggccg gttccgggat cgcctcggga
     3001 gccggctcgt acgccggctc cggggccgcc tccgccgcca cgggctccgg cacctcgacg
     3061 gccggctgcc cgggcagcgg ctcgaccggc aacggctcgg atggggccgg ctcggatggg
     3121 gccggctctg ggtcgtcggc aatcgcaacc ggcgccgcgt cgtcgccggc caccgcctct
     3181 gccgaggccg caggtgcgtc gtccacatcg tcggccgagg gcgcctccgt ggccgccgcc
     3241 tcgtccgtcg ggcgacgggt ccgccgccgc cgccgcggct tccggggctc ggccggcggt
     3301 gctgccgcgt ccaccgcttc cggcgacgcc gcctcgacgc cgtccggcga cgcggcctct
     3361 gccgggacca ccaccgtgtc gctcacctga gcctccgccg ggccatcggg agcgctcgct
     3421 gccgggctcg acggttcctc gtcggcctcg ccttccgcgg ccgcatcggc acgggccgac
     3481 gcctcgccgc cattctcctc cggccgccgc cgccgacgac gacgacgccg acgccgacgg
     3541 ccgccttcct cgtcatcctc gaccttcgcc gtctcgcgcg tccccgtcgg cgcccgctcc
     3601 accggcctct cgtccgcctc gtcggcgtcg tcggcgtcgt ccgcctcggc cgtcgccggc
     3661 tcgaccggct cctcgaccat ggccggccgc ggcggcacga cctcgtgggc cgcccggcgc
     3721 tgaaccacct cgaccgaatg gtgacccggt gtcatctccg gatccacgac gatctcgacc
     3781 cgcatcgcgt agcgctgctc cacccgcacc agaccgtcgc gcttctggtt cagcagatag
     3841 agcgccacct cgcccggcaa ggtcagccgc agcaccttgg cctcgttgcg gatgccagct
     3901 tcctcggcga gcctgagcgc ctgcaacgcg caggattcgg tcgagcgcac ggagcccagg
     3961 ccctggcagg tcgggcaggg ccgcgagctc agctcctgca ggctcggccg cagccgctgg
     4021 cgcgacagct cgagcaggcc gaacatgctg atcttgccga gctggatcct tgcgcggtcg
     4081 atcttcagcg catccctgac ccggcgttcg accgcgcgct gatggcgcat ctcgcccatg
     4141 tcgatgaagt cgatcaccac gagcccggcg agatcccgca agcgcagttg ccgggcgatc
     4201 tcgtccgcgg cctccaggtt ggtcttgacc gcggtctcgt cgatgtgccg ctcgcgcgtc
     4261 gacttgcccg agttgacgtc gatcgcgatc agcgcctcgg tggcgtgcat gacgatcgag
     4321 ccgcccgacg gcaactggac gatcgggctg tgcatcgcgt cgagctggtc ctcgaccttg
     4381 tgcttgaaga agagcggcag ctcctccttg tagagcttca cgtgacgggc gcgggacggc
     4441 atcatcatcg tcatcaggcg cttcgccgac ttgtgcgcct cctcgccctc gaccagcacc
     4501 tcgtcgatgt cacgactgta gagatcgcgc atcgcccgct tgatgaggtc gccttcctcg
     4561 tagatcaggg ccggcgccac gctctgcagc gtggtgtcgc ggatgtcgct ccagaggcgc
     4621 atcaggtagt cgtagtcgcg cttgatctcg gcccggctgc gctgctggcc ggcggtgcgc
     4681 acgatcaggc ccatgccctg cggcacatcg agctcggcga cgatcgcctt gagcttgctg
     4741 cggtcctccg agctgctgat cttgcggctg atgccgccgc cccgggcggt gttgggcatc
     4801 aggacgcagt agcggccggc cagcgacagg taggtggtga gcgcggcgcc cttgttgccg
     4861 cgctcctcct tgacgacctg caccagcatg atctggcgcc gcttgatcac ttcctggatc
     4921 ttgtatgagc gcagcagctt ggcccggcgg cgggcctgct cgctcatgac atcggcggtt
     4981 tcctcctcgg aatcctcgcg gacccggatc ggccgctcgt cgtcgtcggc ctcgccgtcc
     5041 cgtgcctcgc cgtaggcagc atcgccctcg gcggcatcgc cctcggcggc atcgctctcg
     5101 tcctccgccg cttcggcccg gcgcttgcgg ccgcgcccgc ggcgggcgtc accgttggcc
     5161 gcgtcggcct cgacgctctc gcgctccagc ttggccagaa gctcacggtc cgcctgcggg
     5221 atctggaaat agtccgggtg gatctcgctg aaggcgagga agccgtgccg gttgccgcca
     5281 tactcgacga aggcggcctg gagcgacggc tcgacccgcg tcaccttggc gaggtagaca
     5341 tttcccttga gctgcttctt ggtcgacgat tcgaaatcga aatcaacgag ctgctggtcc
     5401 tgcaacacga caacccgggt ctcttccgcg tgtgtcgcat cgatgagcat gcgtttggtc
     5461 attgaatgga atctcccgcc ctgagtcgcg gccggcgccg gggctgcggc gacgaaacgg
     5521 gcgtctgatg gcgcaccacc gcgctggcgc ggtcggcggg ctggttgtga tgcgacgcgt
     5581 cgccaccgtc acgcggcatg ccgaggcatg ccggacggtc gccacgcgtc gagccatggc
     5641 agtctcgagt ttcggggcga cgttcgcgtc gcggccggcg ggccggccgc cattgctggc
     5701 ggcctcctgg tcgaacgccg gccgcagcct cgtgccgacg ggtattccgc cggtcgatcc
     5761 cgcgatgtcg ttgagaaggc tcgacaggtg agcgggcaac gccatgcgga cgacaggcga
     5821 agcagccggc ctggccactc gactcgttcc tgtcattgcc ctactcgtac catggacggg
     5881 actataataa ctgtccctgg cgactacaat gcgataatca gcccacgaca tggagtagcg
     5941 acgggttgcg gatgcaccag atacggttcc acgcatcggc cggccggtcg gcgacgcggt
     6001 tgctggcgac cggccttgcc ctcttcgcca cggtcgtcct cctcctcttc ggcagggcgc
     6061 tggcgagcga aaccccggag gtcgtgggcg tgcgcttcgg catcaacgag gcggtcaccc
     6121 gcgtcgtcct ggacgtggac cggccagtcc gcttcggcgc aacggtgctc gccgatccac
     6181 cgcggctcgt cgtcgacctc gacgacgtgc gctggcgggt gcgcgagcac ccgcagaacc
     6241 gtccggtcgg cctggcgcag agcgtgacct acggtcggct cgggccggag cgggcgcgac
     6301 tcgtcgtcga tgtcggccga ccgttccgca tcgtccgtca ggagcagttg gcaccatcgg
     6361 ccgaggttcg ccaccatcgc atcctgatcg acatcaccgc cctgccgcca ccggcgccgg
     6421 ctgccgtcgc tgccgcgccg gcgaccgccc ggccggcggc cgccactgcc cgtgcgccgc
     6481 aattcgccag gccgctgccc cgcccggtgc gggtcgcccg ggcgacggcc gacccggccc
     6541 tcggggcgat cccggccccg ccgagcgagc ggcccgagga cgtcaccctc gcggcggttc
     6601 cggcggcacc gctgcgcccg gccgacccgc cgctccgccg ccgggtcgtc gtcatcgacg
     6661 ccgggcatgg cggcatcgat cccggcacca tcggcatcaa tggtgtgccg gagaaggtga
     6721 tcacgcggca gatcgccgac cgcctgaccg agctgatcga gggaaccggc cgctacgacg
     6781 tggtgctcac gcgccggggc gacgagttcg tggccctgcg tgatcgcatc cagcgcgcgc
     6841 acggggccaa tgcctcgctc ttcatctcca tccatgcgga cagcctcgcc gatgcgcggt
     6901 tgcgcggggc ctcggtctac acgctctccg acaccgcctc ggacagcgag gcggcgctcc
     6961 tggccgccaa ggagaacaag gccgacatca tcgccggcac cgatctctcg gcgcatgacg
     7021 aggtggtggc gggcatcctg atcgacctgg cgcagcgcga caccaacaac aagtcgatcg
     7081 agttcgccga cgtgctcacg gccgagctcg gcgaggtcac caggctcctg cgcaacaccc
     7141 gccgcttcgc cgggttcgtc gtgctgaagt cgccggacac cccctccgtg cttctggagc
     7201 tgggctatct ctccaacccg accgatgccg ccaacctcgc cgatcccgcc taccagaagc
     7261 ggctcgccga ggcgacgctc agggcgatcg accgctactt cggggtgacg caggcacgca
     7321 gttgatgccc gaccattggc gatcggccac gcctcgacca tttgagaggg cgacggacat
     7381 gtcgccggtg gacaggggtc cgccccgggc tgtacgtccg gtgccgctga cgccggtcgc
     7441 tgccgtcccg tcgccgatcg gcggtgcccc gatcggcggt gccccgttcg gtgctgcata
     7501 gagaaggaat gagcgtcttg cgctggctcg tcaaagccct ggcctggctc ggcgtcaccg
     7561 cggcggtctg gctggtgatc gccggagcgg gtgtcgccta tatcgtgcac cgctatgccg
     7621 ccgacctgcc ggatttcgcc cgtctcgcgg actaccagcc gccgaccgtg acccgcatcc
     7681 atgccgccga cggccggctg ctcgccgagt acgcgcacga gaagcgggtc ttcgtgcccg
     7741 tcgacgccat tccggccatg gtcaagcagg ccttcatctc ggccgaggac aagagcttct
     7801 accagcaccc cggcatcgac cccgtcggca tcgcccgggc ggcgatcgac aacttcaagg
     7861 cgctcggcac cggccagcgg ccgcagggtg cctccaccat cacccagcag gtggcgaaga
     7921 acttcctgct caccaacgag gtctcgctcg agcgcaagct caaggaggcg atcctggcgc
     7981 tcaggatcga gcaggccttc accaaggacc gcatcctcga gctctatctc aacgaaatct
     8041 tcctcggccg ccgctcctat ggcgtggcgg cggcggcgct cagctatttc gacaaggcgc
     8101 tcgacgagct gaccctggcc gaggcggcct tcctcgcggc cctgccgaaa gcgccctcga
     8161 cctacgaccc cgaccgcgct cccgagctcg cccgcggccg ccgcgactac gtgctcgacc
     8221 ggctgctcgc cgacggggcg atcaccgcgg ccgagcatgc ggcggcgatc gccgagcccc
     8281 tggtcacccg cagccgcgac agcatcgact atgccagggc cgatttcttc accgaggacg
     8341 tccgccgcca gctcgtgcgc gagttcggcg aggacggctt ctaccagggc ggcctgtcgg
     8401 tccggaccac cgtcgatccc gagctgcagg ccaaggccga caaggcgctc cgcgagggcc
     8461 tcgccaccta tgaccgggag cgccagggct ggcgcgggcc cctgggtgtg ctggacctcg
     8521 ccgccaccac ggactggccg gccgcggtgg ccagcttcga tccgggcttc gaggtcgagc
     8581 cgtggcgtgt cgctttggtg ctcgaggccg gcagcggtgc cgccgggatc ggcttcgccg
     8641 atggcagcag gacgtcgctg gccctcgccg acgtgcgctg ggccacgccc gagctgggtg
     8701 atggcgaatt cggggcaccg gtcgacgcgg tgagcgatgt cctggcaccg ggcgacctcg
     8761 tcgccgtcga atgggtcacg gacggcgagg aggcgggcgg gcgctgggcg ctccgccagc
     8821 ggccctcggt cgagggggcc ctggtggcgc tcgatccgca taccggccgc gtgctggcga
     8881 tgagcggcgg cttcagcttc cgccagagcc agttcaaccg ggcgacccag gcctatcggc
     8941 agccgggctc ggcgttcaag ccgttcgtct acctggcggc gctggagaac ggcttcaccc
     9001 ccgccagcat catcctcgac gcacctctgg tgatcgacca gggccccgac ctgccggtct
     9061 ggaagcccga gaactacagc gataacttct acggcccgag cacgctgcgc gtcggcgtgg
     9121 agaagtcgcg caacctgatg accgtgcgcc tcgcgcgcga gatcggcatg gacaaggtgg
     9181 tcgacgtcgc ccgccgcttc ggcatcgacc gcggcctcgg caccaatctc gcggcctcgc
     9241 tcggcgccaa cgaggtcgac ctgatgagcc tgaccgaggc ctatgcgatg ctggtcaatg
     9301 gcggccgcga gatcgagggc cggctggtcg agcgcatcca ggaccggttc ggtcgcacgg
     9361 tgatgcggcg cgacgagcgg ccttgccccg attgccggaa gctcgcctgg gacagcgagc
     9421 cgccgccggt cctgcccgat acccgcccgg tggtggagga tgcgccacgc gcctaccaga
     9481 tggtcagcat cctcgagggc gtggtgcagc gcggcaccgg ggtcgcggcg agccggatcg
     9541 gcaagccgct cggcggcaag accggcacca ccaacgattc gcgcgatgcg tggttcgtcg
     9601 gcttctcgcc ggatctcgtg gtcggcgtct atgtgggctt cgaccagccg cgcaatctcg
     9661 gtcgcaagac caccggcgcc aatgtcgcgc tgccgatctg gatcgatttc atggcggccg
     9721 ccctcgagga tgccccggcg acgccgttcc gcaccccgcc cggtgtgcgg gtcgtgcgga
     9781 tcgacgccga taccgggctc ctgcccggcc ccgccacgac ctcggtgatc gccgaagcct
     9841 tcctgccggg caccgagccg accgaacggt cgaccggtgc gccgaccgcc gcgaaccccg
     9901 atccgaacgg ccaggcgctg cccagcctgc cacgcacggc gggtggcggc agcgccggcg
     9961 gcctctactg accttcccct ccagttccct gccaacgagc gaaagcgaga ttttgccatg
    10021 cgggccgaga tcatcaatct gaacgacgct gtcgagcagg gcctggtcct gctgcggagg
    10081 catctttgac tgggacaatg cccggcgacg tcgcgacgag ctggacagcc tgagcgagga
    10141 tccgaacctc tggaacgacg cggagaaggc gcaggcgctg atgcgcgagc gccagcatct
    10201 ggccgatgcc atcgacggcc agctcgccat ggagcaggcg cgggccgatg cgctggagct
    10261 cgccgagctc gccgagctcg aggacgacga cgccacgctc gactcgatcg aggcggagct
    10321 gcgcggcatc aaggcgcgga tcgacaagct cgccctgcag gccctgctct cgggcgaggc
    10381 ggacggcaac aacgccttcg tccaggtcca ggccggggcc ggcggcacgg agagccagga
    10441 ctgggcggag atgctgctgc gcatgtatct gcgctgggcc gaggcgcatg gccacaagat
    10501 cgagtggatc gaggagaatg ccggcgagga ggccggcatc aagtcggcga ccatcaaggt
    10561 gctcggcacc aatgcctatg gctggctcaa gaccgagtca ggcgtgcacc gcctcgtgcg
    10621 catctcgccc tatgacagcg ccgcccgccg ccacaccagc ttcgccagcg tctgggtcag
    10681 ccccgaggtg gacgattcga tcgagatcga gatcaacgac aaggatctca aggtcgacac
    10741 ctaccgggcc tcgggcgccg gcggccagca cgtcaaccgg accgattccg ccgtccgcct
    10801 gacgcacctg ccgaccggca tcgtcgccgc ctgccagaac gaccgcagcc agcacaagaa
    10861 ccgggcgacg gccatgaaca tgctcagggc caagctctac gacctcgaat tgcagaagcg
    10921 ccgcgccgag caggacgcca ccgaggcgac caagtcggac atcggcttcg gccaccagat
    10981 ccgctcctac gtcctccacc cctacaagat ggtgaaggac ctcaggaccg gcaccgagag
    11041 ccccaacccg accgcggtgc tcgacggcga cctcgacccc ttcctcgaag cctccctcgc
    11101 ccagggcgcg gggcacacgc gcgtcgagga ggcgcagtaa ccgacaaggg atgcgaggag
    11161 gcacggcctc ctcgcgctcc accggaatat tggctggtgg tgttggccgg cctcagcctt
    11221 caccgacgac gaggaactcg atgcggcggt tgcggctgcg gttccagggg gtgtcgttgg
    11281 gggcgacggg gcgggtttcg ccgtagccga tggactggag gcgggcggcg gggacgccgt
    11341 gctcctcgag gtaggtggtg acggagcggg cgcgggcttc ggacagggcc tggttgagct
    11401 cgtccgggcc gacggaatcg gtatggccgg cgacccgcac ggcgacgccc gggcagcgct
    11461 cgacgatggc gaccacggta tcgaggagcg gccggctctc ctggtcgagc acggcgctgc
    11521 cggtccggaa gtagatggcc ccggtgcgcg acatgatgtc gaagcggccg gcgcattcct
    11581 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagggga tgaccgagac
    11701 cgaggccgct tcctccagct tcttcaggcc atcggtgagg tcgaaaagct cggtgccgat
    11761 cgagatcggc cggctcgagg cgaccgagac gagctcgtcg gtcaagagcg tcagcacgac
    11821 ctcggtttcc agctccttgg tgatgccgtg caggtcgagc tcgaacggca gggtcaggtt
    11881 cagccggcgg cggctctcga ggtcgccgag catcgcccgg tcgagctgca cggtgacgac
    11941 cgcttcagga tatttgaacg tctcgaagaa gaggaagcgc atgcggacat tgcgcaggtc
    12001 gacgcccgtg tcgatgctgt tgagctggat ggtgacggtc gcctggccat ccggcgcgat
    12061 ggcgccctcg aaggcggcaa acgtgctgga ttcgaccttg gtctcgttct tcaccgactg
    12121 gacgtcgagc ttggagagct cgggcgccat ggtccagccc ggggcgaagg gttcttgtgc
    12181 cacggcaccg gcaccggaga gcaccagcag cgcggcgaga agaagagctg cggggccgcg
    12241 accgcgcgcg gcaatcgcag caggactggc agaaatcatc cgttcactcc ctgaacgtcg
    12301 ttcgccggat tgtcgagact agagaggtcg cacggtcgac ccgtgcatgc aagtcgtcgg
    12361 cggcacttga ttttccggta gtgccagcga taccttgggg catgcgctgg ttggcattga
    12421 agtcaggatt ctttcgttgc gtcggggtgg tgctggccgc cctggccggg gtggcgactc
    12481 tggcgcatgc cggcacggcg cgggccgagc agcgcatcgc cctggtgatc ggcaacgccg
    12541 cctacgagat caaccccgcc ctggacaacc cggcgcatga cgcgcggctg atggcggaga
    12601 cgctcggctc cctcggcttc gaggtgatcc agctcatcga cgccgaccag gcgacgatga
    12661 aggacgccat cgccaatttc ggtcgcagcc tcagggcggg cggcccggac acggtcgggc
    12721 tcttctacta tgccgggcac gcggtgcagg cgaagggcaa caactacctg atcccgatcc
    12781 gcagcacgat ccgcgacgag gcggatctcg acctcgtcgg ggtcgagggg cgctgggtgc
    12841 tgcggcagat ggaatcggcg cgcaacatca ccaatatcgt cattctcgat gcctgccgcg
    12901 acaacccgtt cgccgaggtg ggcgcggtcg agcagggcct ggcgcgcatg gatgcgccga
    12961 ccgggacctt catcacctat gcgaccgcgc ccggcagggt ggcttacgac ggcaccggca
    13021 tgaacagccc gttcacggcc gccctcgccc aggcgatgac ccagcccggc gtgccgatcg
    13081 agcagatgtt caaggatgtc cgggtggcgg tgctggaggc gaccaacggc gcccagacgc
    13141 cctgggacag ctcgtcgctg gtgcgcgact tcaccttccg gccggccgag atgccgacgg
    13201 cccaggagca ggccgagcgg cggctgtggg aggcggtcag cgccacgcgc gacccggtcc
    13261 agctcgtgct cttcctgcgc aattacccgg acagccccta tgccgacgag gcgcggagcc
    13321 tcgtcaacga ggcgctgctc ggcgtgtcgg ccgagcctgt cccggcgccc ggagagacgg
    13381 ggtcgggcgc tgccgaggtg gaggtcgagc tcgccgcggt gccgggtgcc ctcggcttca
    13441 gcctgccgct cgtcagcgac gacccctcga tctccggccg gtcgatcgag cagatcatca
    13501 agggctcgcc gaagttcacc ccagtcgagg gtctcgacga cgcggcctgg aagggccaga
    13561 cctgcagcac ctgccaccag tggacgaagg aggcgctctg cgaccaaggc aagttctacg
    13621 tcaaggaggg cgaggcgcgc acgcagtcga agctgcatcc gctgggcggc gccttcaagc
    13681 tggcgctgtt cgagtgggcc ggcgccgact gcccctgagg tcgttggccg ccaattcatc
    13741 catccggcag agcgccagcc acgcctcgat gaccgcgcgg ccgaagcgct cggcgcgctc
    13801 ctggtggcgg acggtctcgg cgccgatgcg cacacgaatg gcggggttgt tgctggcggc
    13861 actgcggatc gcggtccagc ggcggatgat ctcggccgtc gcacccggat ggaagcgcag
    13921 accgtagacg cgatggccga cgcggaaggc ctgggagcgg gcgcggccgc agacggccag
    13981 gatcgcgact tcgtccggca gatccactgc atcgtcgtgc cattccatga acggcaggcc
    14041 ggagccgagg gcacggagaa ccgggtcggc ttcggtcagg gcggtcgggg tcaggtcgac
    14101 atagttgaac tcgtgcgacc cgccggcccg gaccgtcccg ccgcaggcga gggccaggag
    14161 ggcggcgccg gtatcgaggg cgaggacagg ctggtcttgg cggacccgct cccggaggac
    14221 tgccacgagc gggcttcccg ggtcgagggc aacggcggca ctaccgagga cgatcaggcc
    14281 ggcggcactg cccaggacgt cgaggggtgt ttcgtcacgg gcgtcgatca tcgtcagatg
    14341 ccgaccggac gcctcggcga cggcttcgat caggcccggt ggcgcttccg tgtcgagctg
    14401 gacgagcagg atgtccgcgg gatcgggcat gctcaatcgg ccagccggta gtcgatggtg
    14461 gtcaccacac gcaccgtctt ggtcacctgc agctcctcgc tctggccggg cgcgttgtcg
    14521 cgcggcagga tctggaagag gccctggctg gcgcgctgga tgccctggat ggcgctgccg
    14581 ctgtcgcggg cgaactgctc ggccccggcg cgggcattgg cggtggcctc ggcgatcatg
    14641 ccgggcttga cgtcgttcag gccggtgaag aggtagaagg ggccgcgcag gccctcgctg
    14701 gtgagaacga cgccggcctc gaccagcttg ccgacgtcct gcgagagcgc cttgatggca
    14761 tccacgtcgg cgaagcggac catcaggttc tcgccgagga tgtagcggct ttcgaccggg
    14821 ccgctgcgat aggcctgggc caaaaggtcc gtgacgtcga tgccctgcac ctcgatggcg
    14881 tcgcgctgga tcccgcccgc ggcgaggaag gcgagcacgg cggcctcggc ccgggcgagc
    14941 gtggcctggg cctcggccag atcgtcgctg gtggcgacga agcgcatggg ccagagggca
    15001 agatcggccc ggacctcgcg ctcggcgagc cccttgaccg tcaccacgcg ctcggcggta
    15061 cggccctggc ggaagccctc gccgacgaac cagccggcgg cggcgaggcc ggcgccgacg
    15121 agggcggcag caacgagacg aacgaccggc tgcatgggcg ggctcactcc tgcaaggctt
    15181 cgaggtcgcg atagaggtcg agggcctcgg ggttggccag ggcctcggtg ttcttgaccg
    15241 gccggccatg gaccacgtcg cgcacggcga tctcgctgat cttgcccgag cgcgtgcgcg
    15301 ggatgtcgcc gacggcgacg atccgggccg ggacgtggcg cggcgtgcag ttggcgcgga
    15361 tgcggctgcg gatcgcctgc tcgagtgctg cattcagcac ggcgccctgg gcgaggcgga
    15421 cgaagaggac gacgcggacg tcgccttccc agtcctggcc gacgcaaatg ctctcctgga
    15481 tttcggggat ctgctcgacc tggcggtaga tctcggccgt gccgatgcgc acgccgcccg
    15541 ggttcagcac ggcgtccgag cggccgtgga tgacgatgcc gcccgttggc gtcagctcgg
    15601 cgaagtcgcc gtggcaccac acaccgggga agcgcgcgaa ataggcgtcg tgatagcggc
    15661 tgccgtccgg gtcgttccag aagccgaccg gcatgcaggg aaagggtgcc gtgcagacga
    15721 gctcgccctt ctcgccggtg acgggccggc cctcctcgtt ccagacctcg accgccatgc
    15781 cgaggccgcg catctggatc tcgccccgat gcacgggtcc ggccgggttg ccgaggacga
    15841 agcaggagac gatgtcggtg ccgccggaga tcgacgcgag ctgcacgtcc gattttatcg
    15901 cgtcgtagac gtagtcgaag ctttccggga cgagcggcga gccggtcgag aggatggcgc
    15961 gcaacctccc gaggtcgtgg gtatccttcg gccgcaggcc ggccttcttg caggcgtcga
    16021 tatacttggc actggtgccg aagacagcga ccttttcctc ctcggcgagg tcgaagaggg
    16081 cggcgggctc gggatggaag ggcgagccgt cgaaaaggac gagcgtctcc tccgcggcga
    16141 ggccgccgac cagccagttc cacatcatcc agccgcaggt ggtgaagtag aagacgcggt
    16201 cgccgggccg cgtgtcgcag tgcaggaggt gctccttcag gtgctggagc agcgtgccgc
    16261 cggcgccgtg gacgatggcc ttgggcttgc cggtcgtgcc ggaggaatag aggatgtaga
    16321 ccgggtggtc gaaggggagc tggacgaagg tcagtggctt atcgacgttg atgcaatagt
    16381 cgccccagga cacgccccgg gggatgccgc cgaggttcgg gtcgctctcg aggaaggggg
    16441 cgacgacggt gcggcggagg cccggcaggc cggccgcgac atccctcacc ttctggcgga
    16501 tgtcgaggcg cttgccggca tagtggtagc cgtcgacggt caccagcacc ctgggctcga
    16561 tctggccgaa gcggtcgagc acgccggcga cgccgaaatc gggcgaggcg gaggaccaga
    16621 tggcgccgag cgcggtcgtc gccagcatgg cgatgatggc ctcggggcag ttcgggagat
    16681 agcccgcgac ccggtcgccg acgcccacgc cgtcggccgc gagctccgcc gcgagggcgc
    16741 ggacctcggc ctggagcgcc gaccacgaga gctcgcgccg cgtgccgtcc tcgccccgga
    16801 agatcagggc cggcgtcgcg tcgtcgcggc gaagcaggtt cgctgcgaag ttcagcctgg
    16861 caccctcgaa ccagcgcgca ccgggcatct tgtcggcttc ggcgagcact gtcccggccg
    16921 gcacgctcgc ctgcacgcca cccagctccc agaccccggc ccagaaggcg gcgggctcgg
    16981 ccaccgacca gcggcgcagc tcctccaccg catcggcgcc ggtgaagccc tggcgcctcg
    17041 ccatcgtcat gatcgccgtg ccgtttgccc gctcggcacc cggctgccag agaatgccgt
    17101 tcatgcctct cctccccgct cgccgccccc atcaagcgcg cccggcgcct gacggcaagc
    17161 ccgggatgaa gtggcgccgt tcttgcatcg aattggcggc ttctctacca gcagcacgac
    17221 aaaccaacag gagcgccagc ccgtgatccg ccgcaccctt ctcgcgacca ccctcttcgc
    17281 cggcctcgcc gcgaccacca cactcgccct ggcccaggac gatccgttca aggtcggctt
    17341 cgtctatgtc gggccggtgg gcgatcatgg ctggtcctac cagcacgacc aggggcggca
    17401 ggcgctggag gcgcatttcg gcgatgcggt cgagaccacc ttcgtcgaga acgtcagcga
    17461 gggcgccgat gccgagcggg tgatccgcca gctcgccagc tccggccacg acatgatctt
    17521 cacgacctcc ttcggcttca tgaacgccac ggccaaggtc gcccagcagt tcccggacgt
    17581 gatcttcgag catgcgaccg gctaccagca ggcggacaat ctcgcgacct atctctcgac
    17641 cacctatcag ggccgctacg tctcgggctt cctcgccggc aaggtgagcc agaccggcaa
    17701 gatcggctac atcgcctcct tcccgatccc cgaggtgatc cgcgacatca atgccgtgat
    17761 gctcggcctg caggaggtgc gcgacgatgc cgagctggtc gtgatctggg tcaacacctg
    17821 gtacgacccg gccaaggaat ccgacacggc gcgcgtgatg atcgaccagg gcgtcgacgt
    17881 catcatccag cacaccgaca gcccggccgc catgcaggtc gccgaggagc gcggcatctg
    17941 ggcggtcggc caggcctccg acatgagccg cttcgggccg aaggcgcagc tgaccgcggt
    18001 gatcgacgac tggtcgccct actatatcga gcgggtccag gcggcgatgg acggcacctg
    18061 ggagacgcac gacatctggg gcggcatgca cgagggcatg atcgtgatgg gcccgatcaa
    18121 cgccgccgtg ccggccgagg tcgccgacga ggccacggcg atcgccgcct cgatcgccga
    18181 tggcagcttc cacccgttca ccgggccgat caggaaccag gcaggcgagg tggtgatccc
    18241 cgaaggcacc gtcatgtcga acgaggagct tgccggcatg aactggtatg tcgagggcat
    18301 cgagggcagc gtgccgcagt agcggggatc aggcgtcgag ctcgaggcgg aggcagaccg
    18361 agccgaccgc ccgctgcatg gtcgcgagct cggcccccgg cacttgcagc gtcatggcgc
    18421 gcccaggcga cgaggggcgc tcggccggca cgagcaccgg caccgtcgtg gcgcgccgga
    18481 ggaaaagtac cagcctgagc tcgtcatagc tcgcctcgac ggtcaggcgg gaatgaccct
    18541 gcggcgccgc gccgtcgcag cggaggagat ggccgagagc caggaccgcc cgggcgagca
    18601 gctcctggcg caggccccaa agacccgcct gtacggcgag aaaggcggcg atctccctct
    18661 ccgcctgcga gaggtcggcc acgacgagcc gggcgcgccg gtgaatgccg atgcgaaaga
    18721 cgaggttgag gacgaccgca acgaccatgc cgagcgctag gcccgagctc gcgaaaggct
    18781 gcagtgccac cggcagggcc aaggcgagct ccggcattgc ccgaaagctg gtggcggcga
    18841 taatggcggc cccgaccacg agcgcccggc gccggtcgag cacgcgcgag ccgatcagct
    18901 caatgccgtt ggccaccatg tagatcgcgg cgaaggcgag caccccgccg agcaccatgc
    18961 cgagcatgag ctcgaacagt gcggcgagct tcggcatgca ggccatggcg gccagccagc
    19021 ctgccagggt gagcgcgatc cagcggctcg tggcgccggt ggcaccggag atgccgacgg
    19081 cgctcgtcgc cgagttctgg ccggtgctgc cgaggagccc ggcacagatc gaggcgagcc
    19141 cgtcgccgaa gacgccccgc cggaggttgc cggcgtcggg tcggctccag tcggcatcgt
    19201 tcaggcgctg cgccgtgctg acggcaccta tggtcttgag ggtggcggca aaggcggcga
    19261 gcccgaaggc aagaagcagg gtcgggtccg ggctgatggt ccaggtgaga tgaccgaggc
    19321 gcggcagggc aagccatggc tcggcgtcga gatgccgcca gccatccggg gcgatggcgc
    19381 ccagcgacgc cgcgagcacg accccggccg ccatgccgat gagggccgca tagagccgga
    19441 aggccgggcc gccgaagacg ctgacggcgg ccgccacgcc gaaggtggcg aggccgatgc
    19501 cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgccga agacgctgac
    19621 ggcggccgcc acgccgaagg tggcgaggcc gatgccgaaa acgatcgcgg cgagcgtcgc
    19681 cgaatcgacg ccggctgccc gggcgacgaa gaccgggacc acgagcgagg aggcgacgat
    19741 cgccatgtgc tgcaggccga gcaggaccag cggtgcggcc ggcggccgtt cgtcgaccgc
    19801 atagatcaag gcgggtggtc gtgcgccgcg catcgacgga gacggggtgt cgactgcatc
    19861 cggcagggaa agcgaaagac ctcccggatc gagcgagctt cttgtcgtca tcgttgttct
    19921 tgctcctgcc gaacaaagta gtcaggagat cgcttcgatt tcccggttat ctgtcgacaa
    19981 gagcaggatg ctggctgtgc gatttgtcac ataagaacaa ttgatgaggc ttacgactca
    20041 actcccgttg ccggccggtt tctgccgacg ccgagccgat ggaatgtcac cggccgatcc
    20101 aggccgtgca gccgggtcgt ctccttctgc tgctcgagcc cggccaggac tgcggccacc
    20161 gccggatcgt cgtgcagggt tgccgagacg acgatgtcgt ggccgcggct ctgtccctga
    20221 aggcgcgccg cgaggttcac cgcggcgccg aaatagtcga gcgtgccggc cgtggtcacg
    20281 gcgatgcatt cgccgcgatg caggccgagc ttgatggtga tggcgttggc cgcgtgcgcg
    20341 gcgttgaatt cggcgacacg cgactggatg gcgagggcgg cccgggcggc attggccggc
    20401 tcggcgaaag ccgccatcac ggcgtcgccg atggtcttga ccaggatgcc gtcatgctcg
    20461 cgcacgtggg tcgcgaagaa ggcgaagtgg tcgcggatca ggctgtaggc tgcgctgtcg
    20521 ccgatgctct cgtagagggc ggtcgagccc tcgaggtcgg agaacatgat ggtgacctgg
    20581 ccgatgacga cgtcgtcgcc gggcctgagc acctgggtcg ggcacagctc gcggaagacc
    20641 ggcatggcga tcacccggtc gccggtgagc gcgtccttcg accagctccg ctcctcgatc
    20701 acgaggttga gcggccggtc gctcgcgttg cgcagcagga ccgtcccggg cccggccagg
    20761 gggccggcca cgaccgtgcc gccgctggcc acgacctctg gaaaggcgcc gcccggatgg
    20821 tcgacgtcgc tctccccccc ggcctcgacc gtgcgcagcc ggtaggcgcc gggctcgatc
    20881 acgagcggca gcgccagggc ggcaccggcc gccaccgcgt gctggaccag cacatgcggc
    20941 gtcgcggccg ggctcatcat gcagaaatcg gcggcgggca gcgcccggag ccagccggcg
    21001 ggctggaaga cgagctccac attggcggcg aagtcccgct cgtaatcgat gttgcagctc
    21061 tcgcagtgca cgccgcgcgg catctcgtag agattggtca ccacggattt gccgacccgg
    21121 cagcgcgggc agaggatggc ccagcgcatc tccaggagcc cggcggcctg ggccgcgacg
    21181 cagagctcga ccaccgccgt ctccggcgca ccccaggccc ttgccagggc cagtgggcgg
    21241 atgcgcttga ggtcggcgcg cgcggcggtg gtgaggtagc cggcgaggcg tgacgtcagg
    21301 ccatgaccaa agggcccgcg ctcgatcacg gccaaggctt cccgcagccg cgcctcgctc
    21361 gccgcctcga gcgggtgcgg cttcggcacg ggtgcgaggg ccggatcgtc gagggcggcg
    21421 atgcgccggt cgttggtccc gatcaggttc gtggcgacgc gggcgagcac gccgaggcgg
    21481 tcgatgacgt tgccgagcag ggagttccag aaggcctcga acacataggt cacgcgcgtg
    21541 ccgccgccgt ccgcatcgag gcggacatgc atgccgatcg tgtcgacggg ccccttcgtg
    21601 tagcggcgca cctgcgagaa cgagcgctgc tcgacccact cgccgaagcc ctcctcccat
    21661 tccacatcga acggcccgag ccggccgcgg ccgcgacgca agaccgtgcc gtccggccgg
    21721 gcggtgtcct cgatggtgta ggcgttgagc ccctcggtca gctcgttgcc gcgctcggtg
    21781 tcggcgacga tggcccagat gtcgaccggc ggccgcgcgt agcgccgctc gaactcgagg
    21841 cggcgttggc gcatcgccgg cggcctaggc cccgccctga gcccgctcca tggcgacgct
    21901 cagcttcggc cccttggcga tggtctggta gacgacgcag tagcgctcgg tgagcttgag
    21961 aagcgtgtcg agctgctcct gcgaagcgtc ggtggcgacg tcgaagcgca ggcggatggc
    22021 ctcgaagccg accggggcct cgcgctcgac gccgagcgtg ccgcgaaagt cgagatcgcc
    22081 ctcggcggtg acccggccgg agcgcagctc gatgccgagg gccgtcgcca ccgccttcag
    22141 ggtcaccccg gcgcaggcga ccagcgcctc gagcagcatg tcgccggagc agagctcctg
    22201 gccggtgccg ccggtggccg ggtggagccc ggcgatctgg atggcacggc cggtctcgac
    22261 cttgcaggcg atggcctggt cgtcgatctc gcccgaggcc ttgagtgtga tccgggcggc
    22321 ctctgcgtcc tcccggtagc ggctcttcaa gggggcctgg agcgccttca gggcggcggc
    22381 gtcgagcatc ggctggtctc ccgtggtttc gtgaggcgac tgctaccagc cgccgacagc
    22441 gacattggcc tgggtgctgg cgcgctggcg ggcgaggccg agcgagccgt ccagagcatg
    22501 cagcacccgg cgctcgatgg cgatgaaggc gtcgtcgagc cggtggcgcg ggcgcggcag
    22561 gtcgatgcgg atctccttga agacgcggcc cgggctcggc tgcatgacca ccacccggtc
    22621 ggcgaggaga atggcctcct cgacgtcgtg ggtgaccagc gccagggtcg gccggctggc
    22681 ctcccagagg gccagcagcg cgtgctgcag gtcggcccgg gtgaaggcgt cgagggccga
    22741 gaagggctcg tcgaggagca gcaccttggg gcgggcgacg agggcgcggg cgatggagac
    22801 gcgctgcgcc tggccgcccg agagctcgcg cggccaggcg ctctcgtagc cggcgaggcc
    22861 gatgcgctcg agggcgcgga caacgcgttc atgacgctcg gccctgggca ggtgggtgag
    22921 gccgaaaccg atattgcccg cgacgtcgag ccaggggagc aggcgcggct cctggaacac
    22981 gatgccgacc gcctcgtgcg gggtggtgat gaccgtgtcc tcgaccgaga ccgtgcccga
    23041 gctcggccgg tcgaggccgg cggcgagcct gagcaaggtg gacttgccgc agcccgagcc
    23101 gccgatcacc gccacgatct cgccggcgtc gagctcgagc gaaaggtgcg tcagggcgtg
    23161 ggtgccgttg gggtagatct tctcgatctg gtcgaagcgc agcatggcgg cgggcggggc
    23221 ggcggctcaa ccggccggcg cgtagccgtc cttccagcgg aggaagggcc gggtggcggc
    23281 cacgagcagc gcgtcggtga gcttgccgag cacggcgaag accaggatcg aggcgatgat
    23341 ctggtccggc ttgccgagct gctggccgtc gacgagcagg tagcccagcc cctgcgaggc
    23401 gcccatgaac tcggcggcca cgacgaacat ccaccccagg ccgaggccgg cccggagtgc
    23461 gaggatgtag gccggcagga tcgccggcag gaggatgcgc cggaccaggg ccgggccgga
    23521 gagccgaaag acccggccga cctcgacgat cttgcggtcg accgagagga tctcgcccat
    23581 cacgccgaga tagaccggga agaagacgcc gacggcgatc agcgccacct tggattcctc
    23641 gaagatgccg agccagagga tgaagagcgg cacccaggcg atcgacggca cggcccgcag
    23701 cccctgcagg gtcgggtcga ccagccggcg cgtcaaaccc gaatagcctg cggcggcgcc
    23761 caccagcgtg ccggcgatgg tgccgatgac gaagccgacg gcgacgcgcc aggtggtgat
    23821 ccagatgtgc tcccagaggt cgccattggc ggcgagcttg aagagcgtcg cgaagatcgt
    23881 gctcggcggc ggcatcaggc ggcgcgaggt gagaccgagc gccgccgccg cttcccagac
    23941 cagggcgagc aggacgggca gcgccaggcc gagcacgagc gggctggtga ggatggcggc
    24001 gaggccacca cccgccctgg cacgccgggc ggcgggcgcg ggacgcccga ccagatcagc
    24061 ggtcagctcg gctctggcca tcgatccggt tctttctccg cccccgcgca cgcacccatc
    24121 ccgttatcgg ctcaatcggc gagcgcttca ccgaagctcg gctcgatcag ctcgtcgagc
    24181 accgcctcga catcgaccga ttcctcgatg acgcccgcct gctggagcgc caggcccgcc
    24241 tcgagaatgg tcgcccgctg ctccgcgccg atccggttat gggtcagctc ggtccgctca
    24301 ccaagctgct tttgcgccac ctcgacggga atcccggcct cctcggcgag gagcgcggcc
    24361 agctcgtccg ggtgctcgag cgcccatttg cggccctcct catagacggc gaggacccgg
    24421 gcgatgatct ccggatgctc ggccgcgaac tcctcgcgga cgttgaggat gccccagctg
    24481 ttggcgtcgg cgttgcggta gaagaggcgc gcaccctcct cgagctcggc cgaggccatc
    24541 atcgggtcga gcccggccca ggcgtcgaca tcgccgcgcg acagggcggc gcggccgtcg
    24601 gcgtgctgca gaaggacgaa gtcgacatcg tcctcgccga gccccgcatc ggcgagtgca
    24661 cgcatcagga agatgtgcgg gtcggtgccg cgggtgaccg ccacgctctt gccctggagg
    24721 tcctcgacct tctcgatcgg gctgtcggcg gtggtgacca gggcggtcca ttccggcttg
    24781 gaatagacgt agatcgactt gatcgggttg ccgttgatgc gcccgacgag cgaggcggca
    24841 ccggcggtcg agccgaactg gatggcgtcg gcgctgagaa actcgagcgc cttgttcgag
    24901 ccatgcgact gcacccagcg cacctcgatg tcgtccttgg cgaactcctc ctcgatcagc
    24961 ccctggttct tcaggaccag gctcaccggg ttgtagaagg cccagtcgac gaccagctcg
    25021 tcgatgtcgg cggctccggc cgaatgccag ggttgcagca ggaacgccgc ggcggcggcg
    25081 gaaaggagca ggcggcgggc gaacgtcggc atggcgtgtc tcccaattac tctattttgg
    25141 ctgtaaaatt tatttttcac agctccgagg tggtggagag ctaggagtcg gcccttctct
    25201 tgtcaagcgg cggtgccggc cggcgggggg cttgcggtcg cgccgacgat gggcgacgct
    25261 gcgccgagct tcacgtgtcg aaggtaaatg tagcgccatg tcgtcgaccg aaaaagtcga
    25321 gctagaccct gccatccgcg acgcctggca cgtctgcgcc gtcgctgccg atattgcccc
    25381 gggggcgacc gccatgcatt gcctcttcgg ccagcggctc gaggtggcgc gggacgccgc
    25441 cgggcgcccg gtcgtcagag gtgacggccg ggtcctgccc gcgctcgagc gctacggctt
    25501 catctggacc tcgcccggca cgccgcccga agatctcttc cccctgcccg gctacgacga
    25561 gcccgatcgc aagaacgtgg tgaccggctc ggtcggcatc cgggtctccg caccccgggc
    25621 agtcgagaac ttcctcgaca tggggcattt ccccttcgtg cataccggca tcctcggcga
    25681 ggagccgcat accgaggtca aggagtatgc ggtcgcgatc gacccggtgg ccgacgaggt
    25741 ggtggcgacc gagtgccagt tctaccagcc gcgcgcggcg gtaggttcga gcggcggggc
    25801 gaagacggaa tatgtctacc gcgtgccgca cccgttctgc gccatcctct acaagacgag
    25861 cccgttcgac gcggtgcgca tggacatcgt cgccctcttc gtccagccgg tgggcgagga
    25921 ggagatcatc ggccacacca tgatgtcgat catcgacgac aggagcacgg cgaccgaggt
    25981 gcgccatttc cagctcacca tcttcggcca ggacaagccg atcctggaga accagcgccc
    26041 gcgcaagctg ccgctcgacc cgcgcgccga gacgccgatc cgcgccgaca agtcctcgat
    26101 catgtatcgc cgctggctga cgtcaaaggg cgtcgcctat ggcgtgatcc ccgcaccctg
    26161 atgctgatcg ccctccaatg ctgaacgacg agcagcgccg ggcctggcag ccggtggcgt
    26221 cgagcaccga tctggtggcc cgccacgtct tcgccggcgc gatctggggc caggagctgg
    26281 ccctttggcg ggccgatgac ggccatgtca atgcctggga gaaccgctgc ctgcatcgtg
    26341 gcgtcaggct gaccatcggc gtcaatctcg gcagcgagct gatgtgccag tatcacggct
    26401 ggcgctacgc caaccgcacg gcgggctgca cctatatccc ggcgcacccg gccgacgcgc
    26461 cggcgcgcac catctgcaac cgggtctacc cggcggtcga gcgccacggc ctcgtctggg
    26521 cggcgctcga aggcacggtc gagggggcgg ccctgcccga gctgccgatg gacgggctcg
    26581 tgctgcggcc cgtcccggtc gacgtgccgc cggccgaggt cgaggcggcg ctggcagcgg
    26641 aattcggccg ggaccgcgtg cgctggctgg tccagccggt cgatgccgga agatcgatca
    26701 tccgcggtgt cctggccgag caaccggcgg agcccctgcc cgtgctgcgg cggtggtcgg
    26761 cccggctcgg ccggctgcgg gatcggctgg aggccctgcc ccggccgccc gtcgcgccaa
    26821 ttccgcctga gctgcaccgg gtcgatcctg ccttggccgg cctgcccgag cccgggccgg
    26881 ccgggacgag cctgcgcctc cgggtggcgc gcaaatggcc ggtggcggag gacgtgacgg
    26941 cctttcgcct ggccccgctc gccggcgagc tgccggcctt ccagccgggt gcccatatcg
    27001 atgtcgccct gcccaacggc ctgacccgcc agtactcgct gaccaacggc ccggacgaca
    27061 cggcggcctg ggtgatcggc gtcaagcgcg agccagccgg gctcggcggc tcgacctgcc
    27121 tgcacgatgc ggtgcgcgag ggtgacgtgc tggccgtttc gccgccgcgc aacaacttcc
    27181 ccctccgccg cgacgggctc ctcacccttt tcatcgccgg cggcatcggt ctgacaccgc
    27241 tgctcgccat ggcgcgaacg ctgcacctcg cccgcctgcc ctaccacttc cacgccttcg
    27301 cccgctccgt cgcccacctg ccgttcgtcg atgtgctgga cggcctcggc gacgcggtcg
    27361 agcgccacat cggcctcgac ccggccgcca ccacggcaaa gctggaggcg atcatcggca
    27421 cccagggcga gaagcacgag ctctatgtct gcgggccgac ccccttcctc gatgccgccc
    27481 gggccatcgc cgccgccaaa ggctggcccg atacggccgt gcatttcgag tacttcaaga
    27541 acgagcgccc acgcgacgct tcgaacgcct tcgaggtggc gctggcacgc tccgcactca
    27601 ccctcgacgt cgctccgggc cagagcatcg tcgaggccgt ggccgcccac ggcatccaca
    27661 ttccgacgtc ctgcgcccag ggtgcctgcg gcacctgccg ggtcggcgtg atagaggggg
    27721 cgatcgacca tcaggacgtg cacctgaccc cgtccgagaa ggccaggggc gactgcctca
    27781 tggcctgcgt cagccgggcg gcggccggtc ggctggttct ggatctttga cagccatgct
    27841 gacgctttac gactacgagc tctcgggcag ctgctacaag gtgcggctga tgctgggctt
    27901 tctcgggctg ccgcacgaga cgcggcatgt cgatttccat cccggccgcg agcacaagtc
    27961 ggacgcgttc ctcgcgatca acccgctcgg ccagctcccg gtgctcgacg atgacggcta
    28021 cgtgctcagg gacgccaatg ccatcctgac ctacctcgcc tcggcgcatg acccgggcgg
    28081 gcgctggggt ttcggcggcg acgcggcgag gaacgggcgg atcgccgaat ggctcggctt
    28141 cgccagcgac ctcacgggga cgatcggtgc tgcccggctg cacgatgcct tcttccacga
    28201 catcgacgcc gatgccgccc gagcgggcgg caagcgcctg ctccgcatcc tcgacgagca
    28261 tctctggttc caggagcggc agggtgcggc ctggctggcg cccggcgatc atccgtcgat
    28321 cgccgacatc gcctgcttcc cctacacggc cctcgcccag gacgccgatc tcgacctcgc
    28381 ggactgtcca gcgatccgcc gctggcacga tcgcttcaag cgcgtgcctg gtttcacggt
    28441 catgccgggg gtgttcccgg cgcgggagtg aaagacgctc tcaagcctcg gcgtcagccg
    28501 cctcgagcgt cgcccattcg aaaacgcgct cctgctgttc cctgaggaac gccgactcgt
    28561 cggtcacctc gtgaccgagc tctcgcgcgg cggcaacgaa gccctgcagg gtgtccggct
    28621 ccatcttgcc gctcttgacg ttgggcttgt tgacgacgat cgcactcagc atcggccagt
    28681 cgcggcgctc ggcatactcg accaggcggc cgagatgctg gcccatcgtc agatgcaccc
    28741 ggttccaggt cgtgccgctg gcgtcggcca gctccttgta gctgacgaac cgccgctccc
    28801 gggccgccgc ctggatgacg tcgaacgatt tctggaactc cagccccttc cccttgcgcc
    28861 gatcgaactc agccagtgcc gccagataca ggtcggcctg gacggctccc tgccggcggt
    28921 gattctcgat caggttcttg agctgttggt cggtctgttc ctgcaggtcg gtagacatct
    28981 tgacttccaa ttgcgcaagc gccgcggaat cgcggcgcga tcttcggctc agcccacgaa
    29041 ctcggccacc agccggttga acttcgtcgg gttctccatg aacatgaagt ggttgccgcc
    29101 ctcgtcctcc tcgaagatct cgagcttcga gcccgcgatg acgctctgga tccagacttg
    29161 cgagcgccag tcgatcaggc tcttgcggcc gccgatgacc agggtcggca gctcgatgcg
    29221 gcggatgacg tcgcgccagt cctgggtggc gtggttgtag agcagcgtgg cggcgtggcg
    29281 gcgcggcagc ttcaggttct gctcgatcgc ccagtcgacc ctcgcacggt cataggcgtc
    29341 ggtgaacatc ccgccgacga actgcctggt gacttcggcc ccgccctggc tggcgaggcc
    29401 gttgatggtg gggtagaggc tctcgtggtc gaggatcggg ccggcggcga ggcgctcctc
    29461 ctcggcccac acgggattgg ccatgatgca gggcatctgg tccaccagca cgagacggct
    29521 cacgcgctcg gggccgaacg tgtccaggta gctccagatc accgagcagc ccatggaatg
    29581 gccgagcagc gtcacctcgt cgagatcgag cgcttcgagc acgtcccgca cgtccttcgc
    29641 cagccgggcg atgcggtagc cgtgctcggg cttctcggat tcgccgtggc cgcgatggtc
    29701 gagggcgatc agccggtagc gatcctgcag gccctcgagc tgggcgccga actgggccgc
    29761 ggtctgcgac cagcccggga tcagcaggat cggcggaccg ctgccggcct cgtcatagtg
    29821 aagagcaaca ccgtcgctcg ttctgacact cggcaccgtg agcctccctc gccagtcggt
    29881 cccttcattt ggacctacca gtcgtgccac ggatcggtgg tatctccaag cccggcggcc
    29941 acggttcacg cgcgccgggc agcggtttcg agctcgtgcc ggtggtcgtc gtcgaggccg
    30001 tggatgtgca gcgccggcgt cgggcacggg tggcggtgga tgatggcggg ctcgagccgg
    30061 ccgccctgca gcacgaccgc gcgggtggcg agccgctcga gcgccgggcg gtcgtgcgag
    30121 acgatcagca tggtggcgtc gagcccggcc agaaggtcca gcatgcgggc ccggttggcg
    30181 tcgtcgaggg cgttggtcgg ctcgtccagc agcagcacct cgggcgccat ggcgagcagg
    30241 ccggcgaggg ccaccagccg tttctcgccg ccggaaaggc ggtgggtgat gcgctccgcc
    30301 agatggtcga ggccgagccg gtcgagggtc gccctcgccg tggcgaaggc ggccttcggc
    30361 gtctcgccca ggttgagcgg gccgaaggcg acgtcctcct gcactgtcgg gcagaagagc
    30421 tggtcgtccg ggtcctggaa gagcaggccg acccgtcggc gcacggtgcg gaaatcgggc
    30481 tcgtcggcgc aggggcggcc gaaggcgcca acgcggccgg ccgtgggctt ctccaggccg
    30541 acgagggcgc gcagcagcgt ggtcttgccg gcgccgttgg cgccgaccag cgccagccgc
    30601 tctccccgtt cgaggcggag gtcggcacca tcgagcacgc ggctcccgcc ccgcgcgagc
    30661 acgacgccgt cgagggcaag gacggcgctc accggatccc gatcacggcg agcagggcaa
    30721 gggcgagaac gcccgtgccg aaggcggcat cggcccggcc gagccggccg ggtgccgcca
    30781 ccggcaacgc cccctgccag aggcgcaggc gcatgccctc ctcgacccgc tccgcccgtt
    30841 cgagggcgcg caccagcgtc atgccggcga gccagccgaa gctgcgcagc gcgtggatgg
    30901 agagcccgcc ccgaaagccg cgcgcccgcg ccgcctcgcg catccggcgg agctccgccc
    30961 gcaggagggc gccgtagcgg aggaccagga ggaggagctg gaccaggcgc acgggcgtgc
    31021 gcagccgggc cagcgcctgg ccgaggcgga tgggctcgac ggtggcgagg agggccagga
    31081 cgatgagcgc cgaggcggtg acccgcagca tcaggccgag cgccagcagg agcccgtcgc
    31141 tggtcgcggc cagcggccct aggtgaagca cggtcgtgcc gggcgtggtg aagggcagga
    31201 cgaggaagag cgctgccatg atcccctcga catgcgcgag ccgccgggcg aggccggccg
    31261 ggcgccagcc ggccgccagc gccaggccga aggcggcggc gacggcaagg aggcccgcgg
    31321 ccgggccatc gaggctgagc acggccagga tgagggcaag tgccccgagc aggcgggtgc
    31381 gcgggtcgag cggtgcgagg agatcccggc tcaagacgtg ctgccacggc ggcgggcggt
    31441 cgcccacagg gcggccccgg cgatgccggc gatcatgccg atcccggcca tgacgtcgct
    31501 gaagcgcagc cgcgcctcgg cttcggccag ggcctcgagc aggggcctcg tctggcgcgc
    31561 cacggcggcc tcgacccgcg cctcgatcag ctcggcgagg ccgtcggcgt cggcggtgca
    31621 ggcctgatcc tcgccggtgt cggacggggc gaagcgttcg gctgccaggg tggtctccgc
    31681 ccggtgcccc tcccccgcgt cgagcgccag gcggagcggg ccgggcccct cataagcgaa
    31741 ggcgaagctg ccgtcggcat cgaccggcag gccggccacg accggcgcct cgccgaccgc
    31801 ggcggtcacc agggcattcg ccgcacggcc gccaccgacg aagaagccgt agccggtgac
    31861 cgtgccggcc tcgaccgtgg cgaagagctt cagttgatgg gcggtggcgg atgccggatg
    31921 gcccgcgacg aggagcgtcc cgagaagcag ggccagcctc atgcggcgag cgccgccgat
    31981 ggtgccagca gctccggccg gaccttcttc aggaagccca cggcgaaggc cgtgaccacg
    32041 ccctcgacca gcatcagcgg caggttggcg aagccgacca gcggggcggc gccggcgagg
    32101 ccctcgcccg agagggtgag cgccagcgcc acgccgaggg tggtgagggc cacggcgagc
    32161 gccgcaccca gccccgccgc gagcatggcc gggccggggg cggcgcgggc gacgaaattg
    32221 cgcaggaggg cgccgaccag cacgccgggc agggcgatgt cgagcgtgtt gatgccgagc
    32281 gtcgtcagcc cgccgacgcc cgccagcacg agctgcagga cgagaccgac cagaacggcc
    32341 ggaaaggcgg cgctgccgat ggtgaggccc atcagcgtcg cgaggagcag atgcacgctc
    32401 gacgggccga gcgggatcat gaccagcgaa acggcgaaga aggcggcggc gaggatggcc
    32461 gtgcgggcaa gccgcgtctc gtcgagctgg cgcaggccga tggccacgcc gatgccggcc
    32521 gcggctgtcc cggcgaggag gaccggccag gcgagcacgc cgtcggcgat gtgcgccatg
    32581 gcccggctca gtccgcgcgc acgacgtcgg tggcgcgaac ccagatgagc ccgccgagct
    32641 cgaccgtggc gggggtgccg tcgggcgccg gcagcgtctc gtcggcctcg gtcagggcgg
    32701 cgaagcccca ccagccggcg aacggcatgg cgtaggcgaa gacaccctgg gcgtccgcct
    32761 tgatcacctg cgagccgaaa gcgtcgttcg gcagggcgat gcggccgtcg ttgacgaacg
    32821 ccacctcgat ctcggcgtag ggcacgggct cgccatgggc ccggacaacg ccccggaaga
    32881 gattgccggt ccagagcccg gtcgggcggg ccagaggctc gatctcgacc ggcaggccga
    32941 gctgctcgtc ccagccttcg cccgaggccc agctgtcgac caccaccttc gcgtaatgga
    33001 cgatgtggcg cccctcggcc ggctcgaaat agggctcggg ctcgacgaag agcaccgcgg
    33061 cacccggctc gggcaaggcg acctcggccc gccaggccga agcgccgtcg acggtgaagg
    33121 gctcgaggct ggcgagaaga tcgacggttt cgccgccccg caccatgccg aagcgccgcg
    33181 gtgccgccat ggccatggcc gggccgtcga aggcggggtg cgtgaacacc agatcgatcg
    33241 tcaccgtgcc gccctcgggc agcacgtcgg cgctcggcag gatggtctgg aaatgcgcgg
    33301 cggcgggcga cgccacgagc cacaggacgc cgacgagacc gaggcgaaac gacaagaggc
    33361 tctcccgagg ctgccggcgc gtatgaagtt tcaagcgaaa gctcacacac gccgcgaggc
    33421 ccgtcaaggc ggccagcggc ggaacgagcc gattgtgcgg accaccggac cgttcgacaa
    33481 tccgggcgaa gcccgctatc gctcggcagc tttccgaccc cagcctcgag gacttccgcc
    33541 atggccgatc catccccgct ccgtatcggc atgatcggcc atggcatgat gggggtctgg
    33601 cacagccaga acctcgacga tctcgccgcc gtgaaaggga cgatcgtcgg ccgccgccag
    33661 gagccgacgg aggccttcgc agccgagcac ggctatgcca gggcgacgac cgatctggac
    33721 tcgatgctgg ccgatcccgc catcgatgcc gtggtggtgg cgagcccgtc cgaggcgcat
    33781 gtcgagcatt gcctcgccgt gctcgccgcc ggcaagcacg tgctgctcga gatcccgatc
    33841 gccctcgacc tcgccggcgc cgagcgcgtg gcggcggccg cggcggcgag cgaccgggtg
    33901 gtcgccgtct gccatccctg gcggcaccgg ccggagttca tagcactccg ggaacggctc
    33961 gccgccggcg aggagaagct cggcatgatc gagggccgct ttctgatcca ccggctcgag
    34021 aatgtcggcg ccaccggcta ccggcgaagc tggaccgaca acatcctctg gcaccacggg
    34081 gcgcacctga tcgatctcgc ctgctggctg atgggcgagg tcgacgaggt cacgggctac
    34141 atggcgccgg tcgacccccg gaccggcatc ccgatgacca ctgcgatcaa tatggcccag
    34201 aaggacggca cctcggcgca cctcctgctg acctatctga gccagctcaa gcacaacgag
    34261 gccatggcgc tgaccgatcg ggacagctac cggatcgacg tccccaggca cgagctgctg
    34321 accgcttcgg gcaccctcaa gatcgatgcc gagccggacc tctgccggcg cgtcatcgag
    34381 gatttcgtcg acgcctgccg ccacggccgg cggccgcgcg cgacgccggc cgacgtcatg
    34441 ccggcgatgc gggcgatgca ggtcgtgcag gacgcctggg acgcccggca tggcgtgcag
    34501 agcctgccgg ggcgcgacct ggcgcggctg ggtcagggcc ggtaggtgcc gaagctccac
    34561 aagcggccct cgcagtcccg ggcggagtac tcgcgcgagc cgtagtcggt atcgcggggc
    34621 tcgatgacga tttccgcccc ggcggcgcgg gcgcgcgcgc aatgggcgtc gacgtcgtcg
    34681 acgacgacat agatgccgat ggggttggcg gcccaggggt tgccgggctc gtcgcgggcg
    34741 ctcccgacca tcaggatgcc gttgccgaag ctgagctcgg cgtgggcgat cttgccgtcc
    34801 tcggtcggat agacggcctt ctccgcaaag ccgaaggcgt cctgcagcca gcgcacggcg
    34861 tgcggcgcgt cggcgtaggt cgtcgcggga aagaggctcg ctgtcacggt cgccttctcg
    34921 ctcattttcg ctcctccatc cggagggccg gtagcggcca gctcgttcgt cgatccctct
    34981 agtagaacat ctgcggaatc gccagcgaca gcgcgggtat gtaggtcacc agcatcagca
    35041 cgatgaacat ggtgagccag aagggccaga gcgccctcgc cacctcgtcg accctgacct
    35101 tgccgatggc gcagccgacg aagagcgtcg tgccgaccgg tggcgtgacg agcccgatgc
    35161 cgagattgag cagcatgacg atgccgaaat ggacggggtc gacgccgatc gccttcatca
    35221 ccggcagcag gatcggcgtg cagatgagga tgagcggcgc catgtccatg aacatgccga
    35281 gcacgagcag catcaggttg atgagcagca ggatgacgat cctgttgtca gagatggtga
    35341 ggaagaagcc cgtcaccttg gccgggatct gcatcagcgt catcaggtag gcgaaggacg
    35401 aggcgaagcc gatcaggatc atcaccatgg cgacggtctt ggtgacctcg accatcatgc
    35461 cgggcatctc gcgccagcgc agctcgcgat aggcgaaggc ggcgacgagg aaggcgtaga
    35521 tgacggcgat cgccgccgat tcggtggcgg tgaagatgcc gccgatgacg ccgccgacga
    35581 tgatgacgac ggtgaagagg cccaagagcg cgccggccat ggtgcgcacc gcctcgccga
    35641 gcggcaccag ctcgcccttc gggtagccgc gccggacgga gaggatgtag gtgaggatca
    35701 tcagcgccac gccgaccatc acgcccggca cgatgccggc gaggaagagc tgggcgatcg
    35761 agaccgtgcc gccggcggcg taggcgtaga tgatggcgtt gtgcgacggc gggatgatga
    35821 tgccctgggt cgagctggtg atggtgacgt tgacagagaa cgcatcgtcg tagccctggc
    35881 gcttcatcat cgggatcatg atcgagccga tcgagctcgt gtcggcgacc gagctgccgg
    35941 agatcccgcc gaagaacatc gaggccagga tgttgaccat ggcgagcccg ccacggatga
    36001 agccgacgaa gacgttggcg aggttgacga ggcgcacggc gatgccgccc tgcgccatga
    36061 tcgccccggc cagcacgaag aacgggatgg cgaggagcgc gaaattgtcg acgccgtcgg
    36121 agaccttgag catcatcgcc tccgccggga tgccgatgta gagagcggtg gcgagcgagg
    36181 cgaggccgag cgcataggcg accggcacgt tgatgaggat catcaggaag aggccgccga
    36241 tcaggatgat cgggcccacg gcgccgccca tcagaccgcc tccccggcac tcggcggtgc
    36301 gccgaggacg aggcgctccc cggcgaagag cagggtgacg aggccgctca acggcacggc
    36361 cgtgtagacg aggccgacgc ggatctgctg gaactcggga tagacctgga accaggtcgt
    36421 ccgcaccagt ccggtgccgt accagatcat ggcgagcccc aggaccatga cgagcaggtc
    36481 cacgagccgc tccgcggcgc ggtgcattcc gttgtcgggc agcaggcgcg tcacgacgtc
    36541 gatgctgatg tggccgccga cccggtagca ggccgccgcc ccgaagaagg tcatctgcac
    36601 ggccagcatg atggcgatcg gctcggccca gaacgagccg agattgagcc agtagcgtgt
    36661 gtagacgctc caggcgatga tcagcgtcat gccgatgatc gcgagtgctg cgaagcccat
    36721 gcagagagcc tgcagcagct ccatggcgcg gagataggcg tgcttggtgg acggcatcga
    36781 actggtccgg cggcgggcgg ggtgcagaag cgaacggggt ggcgcgagcc accccgtcag
    36841 agattttcga aaggctcttc cgtccccgtt actggaccgc ctcgatcttg ccgagaattt
    36901 cggcgtattg ctcgccgaac ttctcgcgca ccggcgcggt cgcctcgatg aacggctgct
    36961 tgtcgacctc gttgatctgg acgcccattt cctgaagctt ggccatcgcc tggtcctcca
    37021 tcgccttcca gagctcgcgc tccttctcgg tggcgacctt ggagcattcg cggatggcct
    37081 gctgctgcgc ctcgtcgagc gtttcccaga cgttcttcga gaagacgaag atctcgggca
    37141 cgatcagatg ctcggtgagc gagtagtagc cggcctgctc gtagtggttc tgggtcaggt
    37201 aggagggcgg gttgttctcg gcaccgtcga ccacgcccgt ctgcaaggcc gtgtagagct
    37261 cgttgaaggc gatcgggatg ccgttgccgc ccatcgccgt catcatgtcg atgaagatcg
    37321 gattctgcat gacccggacc ttgaggccct gaagatcctc gggcttctcg atcggccgct
    37381 ccttgttgta gaaggaccgg gcgccggcat ccatgaagcc gagcccaatg aggccgccgg
    37441 cctcgagccg ggccagcagg tcctggccga tctcgccgtc gacgacctcg tgcgaatggt
    37501 cgatcgagcg gaagacgtag ggcaggttga aggcgttgaa gtcgtcgacg atgggcccga
    37561 ccacgccgac cgagacgcgg gtcatctcga gcgcgccgag ctgcacctgc tcgagcgcct
    37621 ccttctcgcc gccgagttgc atcgagtgga agatctcgat ggacatgtcg ccgttcgtct
    37681 tctcttcgag gcactcaccc atggcggcga tggcgtcagt ggtcgggtag cccatggcgt
    37741 ggacgtcgct cgacttgatc acgagcgcct cggccgaagt cgccgcggcg aacgtcacgg
    37801 cgaccgccgc gaccgtgccc tgcaatgcca gtcgcatgac cagcctccct gttgcttgtt
    37861 tgcgatgccg cgttattgca cctgacgtca tgctcgtcga gagctgccgt gccgcgccgg
    37921 cggcactcct tgatgcgcct cttgcccgca taacgatgct gcgccacaag ggcgcggagc
    37981 cggacgaccg ccagggagcc gcgttgcttt tcgccgatcc gccagcgatg cccggacctt
    38041 gagccgggca cggacgagcc cgcgacagct cctggcctgg gcggtctacg actgggcgag
    38101 ctcgccctat ttcgcggtga tcctcacctt cgtcttcgcc gcctacttca cgcagggcgt
    38161 ggcggcgaac ccgatcgacg gcaccgccca gtggggcctc gccatgtcga tctcggccct
    38221 gctgatcgcg tgcctgagcc cggtgctcgg ggccgtcgcc gacgcgggcg gccggcgcaa
    38281 gccctggctc gccctctgca ccggcgtcgc cgccgtcgcc atcggcctgc tctggctggt
    38341 ccggcccgag cccggcttcg ttctcctggc gctcgttctc atggtgatcg ccaacaccgt
    38401 gctcgagctc ggccaggcct tctacaacgc catgctcgcc gacctcgccg accctacaca
    38461 gctcggtcgc tggtccggct ggggctgggg cctcggctat ctcgccggca tcgccgccct
    38521 cggcctcgtg ctcgtcctct tcatccagcc ggacccgccg cccttcggcc tcgatgcggc
    38581 gacggccgag cctgtccgca tcgtcggccc gctgatcggc ctctggctcg tcgtcttcgc
    38641 cctgccgctc ttcctgttca cgccggacac gccgacacgc ggcatcggct tcggcgccag
    38701 tgtgcgggcc ggcctcggcc ggctgctgga gacgctgcgg gcgcttcgcc agggcagcca
    38761 ggtgacgcgc tttctcgtcg cccggctgat ctacaatgac gggctgaaca cgctcttcgc
    38821 cttcggcggc atctacgccg ccggcacgtt cggcatggcg acggacgaga tcatcctctt
    38881 cgccatcgcc ctcaacgtca cggccggcat cggctccttc gccttcggct tcctcgacga
    38941 tcggctgggc tccaagcgga ccatcctgct ggcgcttgcc gggctcgttg tcttcggtac
    39001 ggcagcgctc ctcgccccgg acaaggcgac cttctggacg gtcggcgtca tggtcggaat
    39061 cttcgtcggc cccgcccagt cggccagccg cacgctgatg gcgcggctct cgc
//
LOCUS       DAOTFI010000008        36691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1245_length_36691_cov_6.765640, whole genome shotgun sequence.
ACCESSION   DAOTFI010000008 DAOTFI010000000
VERSION     DAOTFI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 36691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36691
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1245_length_36691_cov_6.765640"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="P5558_01425"
     CDS             complement(<1..522)
                     /locus_tag="P5558_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23002.1"
                     /translation="MSALAGARTPAQGVGETPPVDRQRRLRRQLRRYWLAVLGAILVV
                     AWIVIAILSPYIAPYPYEQIDLTQRLLPPSAEHWLGTDMLGRDVFSRLLVGSRISLLA
                     GFSVVLIGAVVGTLIGGIAAYAGGRIEELMMRLTDLVLCFPPIILAMAIAAALGIGAT
                     NTVIAMLVVWWPKF"
     gene            complement(519..1532)
                     /locus_tag="P5558_01430"
     CDS             complement(519..1532)
                     /locus_tag="P5558_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23003.1"
                     /translation="MIRYLITRLASMVVMIVLATLVVFLISHLVPADPILAQLGDMQA
                     ANPAMVAAYRARWGLDLPVWQQYLIFLEGLVHGNFGVSISTQRPVLDDILQYAPATIE
                     LSTIAFLLSVIVGVPLGVIAAVRRDSWIDHVARLLSLIGVSAPTFWLAFVVLALFYGG
                     LQIAPGPGRLDVVHLPPPFVTGLYLVDSALAGQWDVFRNAAAHLILPSTVLAATTLGL
                     ITRTTRAAMLEALGQDYVRVGRAKGLKERTIIIRHALPNALIPVVTLGGLAYAVLLSG
                     TVLTETIFSWPGLGRYTFRSAVSLDFPAIMGITFLVAVVYLVINLIVDLSYALLDPRV
                     VRQ"
     gene            complement(1570..3141)
                     /locus_tag="P5558_01435"
     CDS             complement(1570..3141)
                     /locus_tag="P5558_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23004.1"
                     /translation="MLVGGLVVAAFAAMAPAQAQDKQVLVLGVDISDARNFDPARMAD
                     YSSPITNGAVYDSLVTQKPGAYEDVVPSLATGWERLDDGQRIRFTLRDDVKFWDGSPL
                     TAEDVKFSLDRLLNVKDQPAVYAQNLEKTEVVDEHTVDLYIKNPAEPIMINLAAPAFA
                     IYSRAMTEAQGGVSTPGAEQEDKATQWLNQNSAGTGPYHMVQWERNAQIMLQKNPHYW
                     GGEVPFERVVIRHIGDSAAQLLALENGDIDAAFNLTTEQLATLEDSADIDIARGLSLD
                     YVYMTLTSSPELNEALGKTENRLAVAHAIDYDGIIEHLMGGNAVRPVNFLPIGVGGST
                     EELLERIGYREDLDKSRELLAAAGNPDGFSFQLSYANAAIVGTSYQLLAQKIQSDLAR
                     VGITVELNPMDQVQLRTQYTGGETQSVITFWNPPSPEPQLWASASIERVAKRVRWEVP
                     QEVKDVVAAAGAEPEPAKRAELYTEYTEMLQAQGNYILLFQPIYRIATSQAIEDFNLT
                     AAGWQVQMGDIKPSQ"
     gene            complement(3265..3714)
                     /locus_tag="P5558_01440"
     CDS             complement(3265..3714)
                     /locus_tag="P5558_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23005.1"
                     /translation="MIDQPYQFDFYDGGGLDLAFLSFAEIDALGDVDVSRFGDRVIGF
                     GGFINISQGAKKVVFSGTFMAHRRRVRGRWRGRLGRPDRRPEARRLHRRRAPSGTLGQ
                     VGPDGPGSVSGPDRVRAIARGDRPEMQASAAGRTARKQLPDAYCLVR"
     gene            complement(3711..4715)
                     /locus_tag="P5558_01445"
     CDS             complement(3711..4715)
                     /locus_tag="P5558_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23006.1"
                     /translation="MNALTTGDLEGRTALFVGAGSTGGIGLATATAMAAAGARVAIAD
                     MASTDVMSLGAEPPGEGHTAHTVDVTDRPSVDRLVAGAQGAIHALVNAAAILRTGAFL
                     DADFDDWTQSFSVNTDGAFHVAQAVARHMVAEGQGGRIVLIASNAARIPRLNNAAYSS
                     SKAAVIQLVRCMTLELGAHGITVNALCPSSTATTMLVDVQAKVDPSRLDGIVKGSIEQ
                     WRTGISLGRVAEPADQAAMCVFLASDGGRFVSPGALRRRCPDLLLLTRVRPGASPRST
                     RSASATRRPRAFRASPIRACSSASSPARWSIPRSSRRWRSTVPPLWASTLAPRPATRQ
                     "
     gene            complement(4712..4843)
                     /locus_tag="P5558_01450"
     CDS             complement(4712..4843)
                     /locus_tag="P5558_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23007.1"
                     /translation="MTDSISTFPVGKVVTFSATVSESDVYLFAGITCSPLFRRQREA"
     gene            complement(4849..5124)
                     /locus_tag="P5558_01455"
                     /pseudo
     CDS             complement(4849..5124)
                     /locus_tag="P5558_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(5205..6272)
                     /locus_tag="P5558_01460"
     CDS             complement(5205..6272)
                     /locus_tag="P5558_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY23008.1"
                     /translation="MSRARRSGTGGKVSLADVAACANVSLATASRVMSGATYPVAAAT
                     RERVLQAATDLSYAPNLIARALVRRMTNTIGVVIGDITDSYFAEIARGVEDQAYRRDF
                     LTIICNTDRSPAVENAYFRLLLDHHAAGIIIAGGAFPQAPENEAFRELAKEASRSATR
                     LVMLTDRDVEAPVISVDDRMVTMDLTRYLIGLGHRRIAYVEGLTGLSTSLRRGEGFAR
                     AMTEAGLDASLRYPGGFGIEPGRAAAALMLRNELPDAVIAASDEAAFGVLTTLQQGGV
                     EIPRQVSVAGIDDNRHAQLFNLTTMRLPTYDLGAMAAKWVIGDEPGAPDSRVTLPCRV
                     IPRGTTSWISTADRKGALRAG"
     gene            complement(6302..7408)
                     /locus_tag="P5558_01465"
     CDS             complement(6302..7408)
                     /locus_tag="P5558_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY23009.1"
                     /translation="MIRRTARCFADGEIRPAAAALDAEEAFPAALYRRMAEVGLFGAT
                     LPEADGGTGADARSYAITMEEVARGYAGLADELGNVEMIGTLLARAGTARQKQKYLSA
                     LLAGEAVSAFAITEAEAGSDVSGIRTTAERHSTGWRLNGEKIWIHNGPNFDFAVVLAV
                     TDKAAGRRGMSIFLVDGDLPGISRGRREHKMGQRSSQVGPVSFKDVELAAEALLGEES
                     RGFHMMMSCLDRGRVGIAGMCVGILQAALDAAVGQAKMRRQFGQPIADFQAVQFLLAD
                     IAKDLHAARALTYEAADALETGEGTTLRCSIAKCFASDAAVARTSDALQIFGGSGYIR
                     GFEVERLYRDARITQIFEGTNEIQRVIIARQLLA"
     gene            complement(7440..9683)
                     /locus_tag="P5558_01470"
     CDS             complement(7440..9683)
                     /locus_tag="P5558_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase"
                     /protein_id="HRY23010.1"
                     /translation="MSKRLLQGGGECCHAEKRAFMIPLVGYANRASVRSGEEICFKVS
                     STSERPYEARLVRVLCGDANPHGPGFSEVEIGATVDGTYPSRFQPVHLGSYARIDGAP
                     GIGDGGIRFRALIWPTLPGRGRQALITRRDVAPGRGASLAIDERGAVSFSLGDGSRTF
                     TAACEAPLRARCWYEVSGGWDAAKKRVWVAFKALRPSLFDPAEGSASVDATPGRWDAP
                     ERPVLIAAAEVRPGDGPAAVGEHYNGKLEAPVVTRIGAGGEESTFAEWDFARGMTTQQ
                     IEDVGPDALHGELVNLPARAMTGAGWAGRIMRWTEAPGQFAAIHFHDDDIYDCGWATD
                     FAWVPPEGFPSGNYAMRLRCGDHEDHIPFAVCPPKGKPTARVAFLVSTFTQMAYCNFD
                     SPGLEARTRQRIAEWGACPYFMEDHPEYGRSTYNHHNDGSGICFSSRLRPNLNMRPRF
                     IAATDPKGSGLRHAAADTYVTSWLEHCGEPYDVITDEDVHNEGVELLRAYRCVVTGGH
                     PEYHTPETLDALQAFIGGGGRLMYLGGNGFYWKVAVHPDLPGVIELRRAEGGIRLWAA
                     EPGEYYHAFDGSYGGLWRRNARPPNRLVGVGFSSQGNYEGSYYRRQPGSFDPRAAFVF
                     DGVGADELIGDFGFFGGGAAGFEVDRADIELGTPPHALVLASSEGHGPMFRLVNEEML
                     RQFPDRPDSEILRADLVFFETPKGGAVFSVGSITYVGSLPYNGYRNNVATITTNVLRR
                     FVDPTPF"
     gene            9927..10769
                     /locus_tag="P5558_01475"
     CDS             9927..10769
                     /locus_tag="P5558_01475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY23011.1"
                     /translation="MANSSDPSMVVLTEADVEAGLRLSGEMGWNQLPADWLLYLRHGH
                     TVGFRRPDGVVVGTGAAMPYECGYGYLALVLVTADWRRRGLGTRLVEHSIDWLEGQGM
                     VPLLDATEAAAEGYRRQAFAPVIELDRWEADLPAAEGAAAGAGPHEIELAMLVGLDAQ
                     AFGATRPHLLDDIRRRPGVRGVVGAEGRGVAMIRPGRRAAQIGPLVAPDDRTAAALLD
                     EVLRGAAGAVIIDVPKARTAFGEALEAKGFSRRRSFLRMARGRPPEPAGPGLLYASTG
                     AEYG"
     gene            10769..11815
                     /locus_tag="P5558_01480"
     CDS             10769..11815
                     /locus_tag="P5558_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY23012.1"
                     /translation="MTLQDPLAIIREGAAIPAHPLALDAARRLAQRRQRALTRYYIDA
                     GAGGLAVGVHTTQFAIREAGLYEPVLSLAAETAADWTDRPLAMIAGVAGRTGQAVAEA
                     RLARSLGYHAVLFSLAAFKGASEDELIDACAAVAAELPLVGFYLQPAVGGVELGTGFW
                     KRFAGIDNVVAIKVAPFHRYRTLDVIRGIAAAGAEERITLYTGNDDHILLDLLTPFTV
                     MRDVEPVTLRFKGGLLGQWSVWTRTAVALFHRCREAARHEAVPAELLALDSRMTDCNS
                     AFFDVANNFHGCIAGCHEVLRRQGLLDGIWCLDPAEGLSPGQAEEIDRVCAAHGDLAD
                     DAFVSENRDRWLAG"
     gene            12029..13036
                     /locus_tag="P5558_01485"
     CDS             12029..13036
                     /locus_tag="P5558_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRY23013.1"
                     /translation="MELLPRVLLLLTERTFQQAFTAAHTERLAGSCRIVRPAPCTSLA
                     RADSLQDVEIVVTGWGTPRLDEATVAALPALRLIAHVGGTVKPLVDPGVLGSSVRITS
                     AVAANARPVAEFALAFILLENKNVAEWVRQYRARRSRLVLGPHSASGTMGNAGKTVGV
                     VGASRVGRCLLHLLRAFDLRPLVHDPYLSAGDASALGAEPCSLHDLLRRSDVVTLHAP
                     SLPATRHMIGASELAMMRDGSLLINTARGEILDHDALVAELRDGRLRAILDVTDPEPL
                     PDSSPFFDLPNVILTPHVAGSLGVEIHRMTELILTEIERFIGTGTLAHEIRREDWATM
                     A"
     gene            13033..13839
                     /locus_tag="P5558_01490"
     CDS             13033..13839
                     /locus_tag="P5558_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRY23014.1"
                     /translation="MSIEPAFLPGLCSVTFRSIDAEQVIALAVEAGARGIEWCSTVHV
                     PPGHLATAMRIAAQCRDAGLDLPSYGTYVRAGADDEQQDMGAALDTAAALGAPNVRVW
                     AGRQGSGVAGEADRARVADAIRAYAALAAASGITLSIEYHRATLTDTLTSTLDLLRRI
                     DHPNCYAYWQPEPGTPLESAAAELRALGSDLSHLHVFHWQAGGARRPLEEAEAYWRRL
                     VRVPGRDSRFALPRYAFSEFVRNDDPDQFRRDFKVLVRLLDPRIAERGDD"
     gene            13961..15853
                     /locus_tag="P5558_01495"
     CDS             13961..15853
                     /locus_tag="P5558_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23015.1"
                     /translation="MTARTVLLGALLVLGPAVAGSEPFKEAPELAALVAEGKLPPVDE
                     RLPTQPLVVSPLSEIGTYGGTWRSALKGASDTGWLRRTVAYDPLVAYSFDWDDVVPNV
                     ALSVDVNDDASEFVFHLREGHKWSDGTPFTAHDLEFAITDAIQIPEYFGASRNTHLLG
                     AKATAVDDLTLKIELPEPNGLFLKQMASVSGMWPLMYQEEYCSQFHIKYNPDADAEAK
                     SAGLTGWPEALYLACDLDNWRDTERPTLLAWKLTSPYDGLSQQVTFERNPYYFKVDSA
                     SNQLPYLDSLQMRQSSSVDDMVLQALNGDIDFMDRHIATTANKPLFIDGQAKGDYRLF
                     PTVQANMNTSIIQLNQNSENPALNALFNEHDFRVALSLAIDRQEIIDVLFVGQGEPWQ
                     ASPRPESRWHNERLAKQHTEFDPDRANALLDGLGLTGRDAEGFRLGPDGKRIAFRLDV
                     TSDVQPTFPDVAEMLAIYWRALGIQLDVRKSERSFVYETMRANRHDAHIWQGDGGLGD
                     AILDPRYYFPANVESVYALLWAKSLYDPGAADALEPPPAVRRQQDLYRELQRSADPVL
                     QDQLFAEILEIAADEFRVIGISLPPNGYGIARNDMGNVPELQPHSWIYPTPGPMGVAQ
                     LFHKRG"
     gene            15939..16922
                     /locus_tag="P5558_01500"
     CDS             15939..16922
                     /locus_tag="P5558_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23016.1"
                     /translation="MARYLARRLLLMAATMAALSIVCFAIIQLPPGDFLSARLAEMAG
                     TGDTVDVANVAWLRAQYGLDEPFIVQYWLWISGVVGGDLGFSFELRMPVAEVIGERLG
                     ISLTVEILAIIVLWAIAVPVGVYSAVRQYSIGDMAATLFGFIGLAIPNFLLALLLMYL
                     SYIAFGVTIGGLFSPEYVDARWSAGRLLDFLGHVWAPVLVIATGGAAQIIRILRANLL
                     DELSRPYVDTARAKGMAEWRLILRYPVRVAMNPLVSTVGWLLPTIISSSIVTGIVLNL
                     PTLSPILLRSLLAQDMHLAGGLILLIGVLTLLGTLLSDILLAWLDPRIRLG"
     gene            16928..18064
                     /locus_tag="P5558_01505"
     CDS             16928..18064
                     /locus_tag="P5558_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23017.1"
                     /translation="MLTRSGDAAPRGPRRPTGTESPWALVWGRFRHHRLALVSGVILA
                     LLYLSAIFAEFLAPGDPQAYNARYTFAPPQSIHLLDRDEDGSLVFRPYVHGYAVTVDP
                     EAMRRTFAIDPDKKHPLRLLGRSEPYLLMGFIPMQRKLLTIEGQGTPLLLLGADRLGR
                     DVLSRLIIGTRVSLSVGLVGVVLSLAIGLVVGGLSGYHGGLLDLVVQRVIEFLRSVPT
                     IPLWMGLAAALPRDWPPLTIYFAITVILSLVGWTELARVVRGRFLALKTEDYVTAARL
                     DGASDLSLVFRHMMPAFSSHIIAAASLAIPAMILAETSLSFLGLGLQAPIISWGVLLQ
                     EAQNLRSVATAPWLLWPAAAMIVAVLAMNFLGDGLRDAADPYAT"
     gene            18061..19809
                     /locus_tag="P5558_01510"
     CDS             18061..19809
                     /locus_tag="P5558_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23018.1"
                     /translation="MTAPPGPAPAAEVLAIVDLETHFPLNRGTLKAVEGVSLTLRAGE
                     TLCVVGESGSGKSVTARSILNIVKPGRIVGGHIWFRPDAVIGAGNGNGPAIDLCALDP
                     DGPRLRAIRGARIAMIFQEPMSALSPMHTIGQQIIEAVRLHRAMDKQEARARAIELLR
                     QVQFREPETAIDRYPFQLSGGMRQRALIAMALSCQPQVLIADEPTTALDVTTQAEILS
                     LIRSLQQRYGMAVLLVTHDMGVVAEMADRVAVMQGGRVIEHGPVDAVFRAPREAHTRR
                     LIEAARLLERRGDRAAGTPAGGPGQAPILSVRGLCLAFRGTTGFLRRRATAVHALRDV
                     SFDVAPGEALGIVGESGSGKTTLARALLGIHRVDAGQARFRRADGSLVDLCARQGYAD
                     RSLYAEIRMIFQDPFAALNPRQTVEQIIAEPLRIHRLCQRSGIGDRVAALLRDVDLSA
                     DVMDRYPHAFSGGQRQRIGIARAMATNPRLVVADEPTSALDGSLRAQILDLLLELRNR
                     LGLSIILISHDIGVVRYFCERMAVMRQGELVEIGASDQICDAPRHPYTAELLAAVPRA
                     GPRHARLRDPDAAGPD"
     gene            complement(20326..23625)
                     /locus_tag="P5558_01515"
     CDS             complement(20326..23625)
                     /locus_tag="P5558_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY23019.1"
                     /translation="MEIAGWLRELGLERYAEAFRAGEIEPEILPELTDGDLAGLGIPL
                     GPRKKLLKAIAALAAGGEAAAGLRAEAAHRAMPPEAERRQLTVMFCDVVGSTRLSARL
                     DPEDMSEVIGAYRHRCTELVGRWGGHVGQYLGDGILLYFGYPEAREDDAERAIRCGLE
                     MRTAFAPILADGEPLAVRIGIATGLVMVGRPIGAGAVREDSVVGEALNLAARLQSLAG
                     PNTIVISRSTRRLVGGRFELADLGVHDLKGFIAPVPAWRVLDESPTVGRFEALQGGRL
                     TPLVGREDELDLLLARWRDAKQGRGQVVLLSGEPGIGKSRIAKALRQQLDAESHVCLR
                     YFCSPFHVSSALHPVLEQVQRAAGFERDDSDSVRLDKLEALLGRATARPAEAAALMAQ
                     ALTLPTGGRYPPLDPSPQRRKEQTFEVLGEQLDGLAARRPVLIVLEDMHWIDPTTREL
                     LDLLVERLVTLPVLLVVTLRDDIESPWGGQPHVTRLPLQRLSLRHSAAMVEGLAPGGT
                     ISARLLEEIVGRTDGVPLFLEELTKTVLESSLPDAADDRFGLAGSPSLAVPATLRDSL
                     MARLDRLASVKEIAQVGAVIGREFSLELVAAALGRPEPEIAAALDRLTASELVFQHGS
                     PLARSYSFKHALVQDVAYQSLLKSRRQQLHGRVARVLVERFPELAEMAPELLAHHFGE
                     ARLPVPAIAYWRIAGERAAARAANAEAVDHLASALRLLPELPEAARPREEVDLQLKLG
                     FVLLALRGFGAEAVGECYWRALALCEELGERQKGLVARFGLWRFHLVRGEYAAADRLA
                     RELQAAAEQDRSAETRLISDWAVGVVSFWLGRLADARLLLRRAAARDTAFQHDLAARY
                     AASPGVTTRLFLSWAEWLAGETAPAQATMAEALALAGPTVPAHSRAWTAVHAAVLHQL
                     LDDVDATRRFAESAIELCEGQRIPHFQAQAKALRGWALAAAGDRETGADEMAGALAQL
                     EKLGNRPMLPYLLTLEAGTLARAGRSGEALARIDAALAESEVSGQAWCNAEIHRVRGV
                     ILGQRDPASVAAEVDLRRALEIARGQGALAWERRAATSLAELYTRIGRADEARALLAG
                     LPVSDPAMAPG"
     gene            23949..25952
                     /locus_tag="P5558_01520"
     CDS             23949..25952
                     /locus_tag="P5558_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRY23020.1"
                     /translation="MPARLHPYLFLTPGRLDRIRQGLDASGSGHDPTVQKWVDKMRRR
                     AREIILPKPILTPDWIAAGEGATAPVMLYTARAAVSRITILGLLWHIDKDGGEADRNG
                     SGLEAQEWLARACRELLAIASFPDWQKPSLPPEVFLSVAEMCAAAAIGLDWLWQHLNV
                     DERRRVQDAIVRNALQPALEQYTIGRATNRGWTTEPYNWNIVCNTGMVLGALMVRDRD
                     PALAEEVMTTALRSVQTGFAGYWNDGGWPEGPGYWSYATRYAAKLLDALRGLDIDDRD
                     LSCRDGLERSGEFVLHCLGPTGMTFNFADSEPRVSLMAMSWLGERFGRDTDSWIASHN
                     PNGSRLALQLLWRAGPGAPPESGRTPRDAAFASIHGAFFRSAWNDPDALWVGFKGGDA
                     RWHHPQLDAGTFVLDAHGLRWAVDLGPSLYANPGYFIRETRSRYYRASTQGHNTLRIG
                     PHQQNDEAVAPLLASWCKDHDDLAGAIVDLSTAYNLPAGELLRGVFLLERRQVLIQDD
                     FRNPPGAEVVWSMHTPATVTIDPERPGVAVLSQERTNEAAPDGTPETCYLSLRILAPQ
                     GAGFSQKSASYAGGTEYPEASINQGITKLQIVLPFDRDAPLERIAVLITPEMAPGKAP
                     SLRPLDDWQGDWQAEPQPQRHWQDDVALHVLEGVERRRNGRGL"
     assembly_gap    25975..26074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26135..27178)
                     /locus_tag="P5558_01525"
     CDS             complement(26135..27178)
                     /locus_tag="P5558_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY23021.1"
                     /translation="MTAAASTTAEVLSVHDLVRHYSLGGGLPGRPRTRVPAVDGVSFT
                     IPAGTTMALVGESGCGKSTTAKLILQLERPDRGEVRLQGERVDGASRASLNRLRRNVQ
                     MVFQDPYASLTPHLRVDAILGEPFKVHRICKAGEIAARVAGLIESVGLSADVGRRFPH
                     ELSGGQRQRIGIARALAVEPALVICDEPVSALDVSIRSQIVNLLLDLQASRGLAYLFI
                     SHDLGLVEHMSDSVAVMYLGRIVETAPASTFFAGPRHPYSAALLASSPTPDPRQRGRP
                     AALRGEIASLVNLPSGCRFRTRCPLAIARCAESEPVLLPVGRGHLVACHRGEEMAGAA
                     ALPARPRSDPTGG"
     gene            complement(27175..28224)
                     /locus_tag="P5558_01530"
     CDS             complement(27175..28224)
                     /locus_tag="P5558_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23022.1"
                     /translation="MSRQGVAPGAGEPVLQLEGVEVRFPSPHGSFAVVDRLSLSVTRG
                     EILGVVGESGCGKSMLARSIVGLVPPPGRTVGGAILFDGVDLVRLSPDERRRIRGDAI
                     AVVPQEPMTSLNPTLRIGDQLTEVLRAHRPEMSRSQRRARALEVMEQVGIQFAGQRLA
                     QYPHELSGGMRQRVMIAMALICGAVKLLIADEPTTALDVTIQAQILELFVRLQSEEQM
                     TVILITHDLAVVAQTAHRVAVMYAGCIVELAPVLDLFDAPLHPYTRGLLETLPRRGAS
                     ASKSHLATIAGAVPDLARPPAGCRFFNRCPHALAGICDAALPALEPVADERWVRCHRW
                     RELPAVAAAAVGAAS"
     gene            complement(28221..29102)
                     /locus_tag="P5558_01535"
     CDS             complement(28221..29102)
                     /locus_tag="P5558_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23023.1"
                     /translation="MTPAAAEATTPSTARSALRAIRRAGPMLLSGGTVLALVVLASLF
                     VPLLVEIDPLGIDVAQKLLPPSAEHWLGTDEFGREVLDRLIIGAHISLRVAFAATLLT
                     AVLGIALGALSGQYRLADSIIGRFSDALLVFPGFVLAIMILAALGPSELNVVLAVVVL
                     YVPRVIRTVRASIIEFRSADFVQAARAVGVGDMRLLVVHILPKSFGPVMVQLTFGFAW
                     AILVEAGLSFLGLGPPPPSPSWGNMISAAREFIRTAPWIMIAPGVMITITVLSLNLIG
                     DGLRDLVDPRLQGKVGP"
     gene            complement(29102..30049)
                     /locus_tag="P5558_01540"
     CDS             complement(29102..30049)
                     /locus_tag="P5558_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23024.1"
                     /translation="MLLYTLRRILEFVPTIFIALTLIFVITRLVPGSPITALIGNQSV
                     DAAQIDAITKQLGLDRPILEQYLEWLPRVVTGDFGQSLFFSAPVAEVILDRFPITFAL
                     TFMALLVTVAVGIPLGILSAMRRGGILDYFGNVLSSLGMALPPFWLGFMLMLVFAVSL
                     QWVPASGYRPMEFGFWSWLSRLVLPVLALSVSQIGLVVRMTRSTMIEVLGMEYVAMAR
                     TKGLRERTVILRHALRNAMVQIVTVIGLVFALGLGGSVIIENVFALPGLGELITTAAV
                     RRDYPTLEGGIFYLTLVALVINLMVDLAYAWFNPRIRYA"
     gene            complement(30111..31721)
                     /locus_tag="P5558_01545"
     CDS             complement(30111..31721)
                     /locus_tag="P5558_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23025.1"
                     /translation="MLRRRTVLVGVVGGLAATSLHLEAGHLEAGRLVRTALAADEPVQ
                     GGTLNVGLHIPLPTLDWQSTVGHPLPHVMGHVYEGLFGLGSDLNAEPELAASAEASDG
                     GRTWTFELRPGVLFHNGDTLTSADVKASLERWRTVGPKGSGLNTLTSIETPDDLTVVM
                     SFSEPMGRYLLLLLGSDENKAVIMPAAIAEASTTPGQLTEVVGTGPYRFVEYKEDQYA
                     RLARFEDYVARDDAPNYQTGKKIANLDEIIFWIVPESTTRVAGLESGEYDVIAEVPDG
                     EATRLAENPDLDAVKNGPGVLLYMMFNHKVGPTAELNIRKAIQSLIDPAEIAAVAVSN
                     PDFALTNPSIYAPESAYNTDAGSDLYTPGDVEKAKEYLAAANYDGEPITIQVIATNSL
                     QNRLAVAIAEQAKRAGLNLEIVSYDLATWVANRRDPAKLNIYTSGGYWVDPSLWHAEF
                     NGTFPSPEVGFISDRTEEIFARLAAETDFDKRKALGEELQRAFYEQVAMVNLGYVYRL
                     VAKQGRVVDPYDNLALGNMTLHGIYLAD"
     gene            complement(31813..32883)
                     /locus_tag="P5558_01550"
     CDS             complement(31813..32883)
                     /locus_tag="P5558_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY23026.1"
                     /translation="MKIDRIDLMPFGGGILVRLRAASGLAGLGEVATDEAFPTMSRAT
                     VSRWLERYGTHIQGAGCGNANVLEQRLDAAEPAGGPGCPPARAALEMAALDLVGRARD
                     CPLHDLLGGGYREDLELFREIDAGDPDPLGTARAAIRDGHAGLRLALPGRSAAAIGAV
                     LDSVGSVPFVDIVARQSLGNEQRASMLVEGLLARQFHLNLALVQPLHRLDFAGHGRLR
                     QKVPIPIVLEESITSPEAMSQVVRHAAADRIVLDPWRVGGLRNARRIANICEAASIGV
                     VVSGPCRSAVGRAALGHLAATIHGSYPICLAPHRGEVEAGLTGGPAFAGGRASLDRGP
                     GLGVVLDEAWAAQAMRAGDLAA"
     gene            complement(32883..33986)
                     /locus_tag="P5558_01555"
     CDS             complement(32883..33986)
                     /locus_tag="P5558_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY23027.1"
                     /translation="MTRSSVTLKDVAAAAGVSVGMASRVLSKYGSYSDATRLKVEAAA
                     HSLGYRANRVARSLRLRRTNTVGVLVSQIASYHWTLFVQGVEEAARQAGYHVILCNTN
                     DDPKREVEYLAEMRERGVDGIITSPLAANRQVFRKLGASGFPAVVVNARIEGGRLVHI
                     TSDDRSAATDAVRHLAGFGHVRIGIVAGPQAFETGRARLGGYLDALEQLGIAADESLI
                     AYGDFRQAGGYTATRHLLLQPLPPTAILVCSELMTGGALRCLKDLGVALPQGLSLIAF
                     DDPDWAAFFTPAMSCLREERFYMGRLAFEALVAAIEKRASPAGQTREIVLKSELIQRE
                     SVAAPRPAPGLVLRAGRADALAPEPGSLNAGHH"
     gene            34110..35309
                     /locus_tag="P5558_01560"
     CDS             34110..35309
                     /locus_tag="P5558_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY23028.1"
                     /translation="MKITAIDLVPLRLEMLSAAVRTGPNVDPADQMDLPPEEIAKLRY
                     APPETVLVRIKTDAGIEGLGDGATLPHYLGHGIGSMLDWLGRFRQVLAGSDPLNIAAV
                     HRRMESVASIGVPGCRPAQAAIDMAIHDIVARAHGCPVYELLGGAFRTELELQTQMHG
                     FSVEQLLGVCRHYLDQGFTGLKLKIAGKLRREGYAKALVDEEAEKIARVVASLPESVQ
                     IDVDANQGLMNAKVAIGLFEHVRRIAPHPNMSIEQPLHHLDLEGHALIRRMLPYPVIL
                     DEAVTSPAAMLQIVRAEAADRIVLKPNRVGGLWPAQKIIDICEAHGIGVSLDTMPFTV
                     LGDTMLCHLGATIRTHYPLDAEGHTFFRETPFVGGVTLQDGRARLSAEPGFGITVDED
                     RLLATAA"
     gene            complement(35451..>36691)
                     /locus_tag="P5558_01565"
     CDS             complement(35451..>36691)
                     /locus_tag="P5558_01565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRY23029.1"
                     /translation="IDFTFTVLDSEVVNAFALPGGYVYVSRGLLAYADDEAELAGVLA
                     HEIGHVTARHSASRQTTGQLATFGLGLLGVLGGAVLGGEEGARLGAQLGQQTGYGLTQ
                     TYSRSQEYEADTLGIRYLDRAGYDTRATADFLQALQTSAELQARLVGASVKEPGAFDH
                     FFSSHPYTPDRVEEARDQADERGSSGDERNRAAFLAAIDGIVYGQSPEQGFVQGSRFA
                     HPGLGIAFAYPEPYRLSNQPTAVVGRADNRLLLFDMGAARQATDPARYLQGEWLKGLE
                     LHGVERFTSDGGLEGAIGYADVQVGGQNAQAGFAVLSGGGREFYRFALITGRFGRNEA
                     RELRAAADSLHRLTKDEAAALRPLRVEIIEVREGDTIESLARRMEVERLPREQFITLN
                     GFDRGRQLEAGDQVKIIVRG"
BASE COUNT         5623 a        12663 c        12795 g         5510 t
ORIGIN      
        1 gaacttgggc caccacacca ccagcatggc gatgaccgtg ttggtggcgc cgatgccgag
       61 ggcagcggcg atcgccatcg ccaggatgat cggcggaaag cacaggacga ggtcggtgag
      121 ccgcatcatc agctcttcga tgcggccgcc cgcgtaggcc gcgatgccgc cgatcagcgt
      181 gccgaccacg gccccgatca gcaccaccga gaagccggcc aggagcgata tgcgagagcc
      241 gaccagcagc cgcgagaaga cgtcccggcc gagcatgtcg gtgccgagcc agtgctcggc
      301 cgagggcggc agcaggcgct gcgtcaggtc gatctgctca tagggatagg gtgcgatgta
      361 gggcgagagg atggcgatga cgatccatgc caccacgagg atggcaccca gcacggcgag
      421 ccagtagcga cgaagctggc gccgcagccg ccgctgccgg tcgaccggcg gcgtttcccc
      481 gacgccctgc gccggggtcc gtgcacccgc cagggcgctc actgccgcac gaccctggga
      541 tcgaggagcg cgtaggagag atcgacgatg aggttgatca ccaggtagac cacggcgacg
      601 aggaaggtga tccccatgat ggccgggaag tcgagggaga cggcgctcct gaacgtgtag
      661 cggccgagcc ccggccacga gaagatggtc tcagtcagga cggtgccgct caagagcacc
      721 gcataggcga ggccgccgag cgtgaccacc gggatcaggg cgttcggcaa ggcgtggcga
      781 atgatgatcg tccgctcctt gaggcccttg gcgcggccga ccctgacata gtcctggccg
      841 agcgcttcca gcatggcggc cctggtggtc cgcgtgatca agccgagcgt ggtcgccgcc
      901 agcacggtcg acggcaggat gaggtgggcc gcggcattgc ggaagacgtc ccattggccg
      961 gcgagcgcac tgtccacgag gtagaggccg gtgacgaagg gtggcggcag atgcacgaca
     1021 tcgagccgac ccggccccgg tgcgatctgc aagccgccat agaacagcgc cagcacgacg
     1081 aaggcgagcc agaaggtagg ggccgaaaca ccgatcagcg agagcagccg cgccacgtgg
     1141 tcgatccagc tgtcgcgccg caccgccgcg atcaccccga gcggcacgcc cacgatcacc
     1201 gaaaggagga aggcgatggt ggaaagctcg atggtcgccg gggcgtattg cagaatgtca
     1261 tcgagcaccg ggcgctgggt ggagatcgac acgccgaaat tgccgtggac gagaccctcg
     1321 aggaagatga gatattgctg ccagaccggc agatctaggc cccaccgcgc gcgataggct
     1381 gccaccatgg ccggattggc ggcctgcatg tcgccgagct gcgccaggat gggatcggcc
     1441 ggcaccagat gcgagatgag gaacaccacg agggtggcca gcacgatcat gacaaccatg
     1501 ctggccagtc tcgtgatcag atagcgaatc aagccatgtc gccttgggcc cgcagcagaa
     1561 cccgctcgct cactgcgacg gcttgatgtc gcccatctgc acctgccagc cggcggccgt
     1621 caggttgaag tcctcgatcg cctggctggt cgcaattcga tagatgggct ggaacaggag
     1681 gatgtagttg ccctgcgcct gcagcatctc ggtgtactcg gtgtagagct cggcccgctt
     1741 cgcgggctcc ggctccgccc cggccgccgc cacgacatcc ttgacctcct ggggcacctc
     1801 ccaacggacg cgcttggcga cccgctcgat cgatgccgat gcccagagct gcggctcggg
     1861 cgagggtggg ttccagaacg tgatcaccga ctgcgtttcg ccgccggtgt actgggtgcg
     1921 cagctggacc tggtccatgg ggttgagctc gaccgtgatc ccgacgcggg ccagatccga
     1981 ctggatcttc tgggccagca gctgatagct cgtcccgacg atggcggcgt tggcgtagga
     2041 gagctggaag ctgaagccgt cgggattgcc ggccgcggcc aagagctcgc gggacttgtc
     2101 gagatcctcc cggtagccga tccgctccag cagctcctcg gtggagccgc ccacgccgat
     2161 cggcaggaag ttgacgggcc ggaccgcgtt gccgcccatc agatgctcga tgatgccgtc
     2221 gtagtcgatc gcatgggcga ccgcgagccg gttctcggtc ttgcccagcg cctcgttgag
     2281 ctccgggctg ctggtcaggg tcatgtagac gtagtcgagg ctgaggccgc gcgcgatgtc
     2341 gatgtcggcg ctgtcctcga gcgtcgcgag ctgctcggtg gtcaggttga acgccgcgtc
     2401 gatgtcgccg ttctcgagcg ccaggagctg ggcggcgctg tcgccgatat gccggatcac
     2461 cacccgctcg aacggcacct cgccgcccca gtagtgggga ttcttctgca gcataatctg
     2521 cgcgttccgc tcccactgga ccatatggta ggggccggtg ccggcggagt tctggttcag
     2581 ccactgggtg gccttgtcct cctgctccgc acccggggtg ctgactcccc cctgcgcctc
     2641 ggtcatcgcc ctcgagtaga tggcgaacgc cggtgccgcg aggttgatca tgatcggctc
     2701 ggccggattc ttgatgtaga ggtcgacggt gtgctcgtcg acgacctccg tcttctcgag
     2761 gttctgggca tagaccgccg gctgatcctt cacgttcaag agccggtcga gggagaactt
     2821 gacgtcctcg gcggtcaggg gcgagccgtc ccagaacttc acgtcgtcgc gcagcgtgaa
     2881 gcggatccgc tggccgtcat ccagccgctc ccacccggtc gcgagggacg gcacgacgtc
     2941 ctcgtacgcg ccgggcttct gggtcaccag cgagtcgtag accgcaccat tggtgatcgg
     3001 cgacgagtag tcggccatgc gggccggatc gaagttccgc gcatcgctga tgtcgacacc
     3061 cagcacgagg acctgcttgt cctgggcctg agccggtgcc atggcggcga aagccgcgac
     3121 gacgaggccg ccaaccagca ttcgcaaggc cgatcttcca ctcttctctc gggcaatagt
     3181 ccgcaagcct ggcatgttgc ctcctcccgt ggttgctttg gtggatcgtg agaccggccg
     3241 agggtcgacc gctgcttgtc ccgactacct taccagacag taagcgtccg gcaattgctt
     3301 tcgggccgtc cggcccgccg ccgacgcctg catttccggt cgatcgccgc gagcaatggc
     3361 cctcacccga tcaggccccg aaaccgatcc agggccgtcc ggaccgacct gaccgagggt
     3421 gccagatggg gctcgacggc gatgtagccg tcgcgcttca gggcgacgat ctggccggcc
     3481 cagtcgacct cgccatcggc cacgaactcg ccgccgatgg gccatgaagg tgcccgagaa
     3541 gacgaccttc ttcgccccct ggctgatatt gatgaagccg ccgaagccga tgacacggtc
     3601 gccgaaacgg ctgacatcga cgtcgcccag ggcgtcgatc tcggcgaagg agagaaaggc
     3661 gagatcgagc ccgccgccat catagaaatc gaactgatag ggctggtcga tcattgccgc
     3721 gtagctggtc gcggcgccag cgtcgacgcc catagcgggg gcaccgtcga tcgccagcgc
     3781 ctcgagcttc ggggaatcga ccagcgtgcc ggtgacgatg cgcttgagca tgcccggatg
     3841 ggcgaagcgc gaaacgccct gggtcgcctc gtcgccgacg ccgaccgggt ggaacgaggt
     3901 gatgcccctg gccgcaccct cgtcagaaga agtaagtctg ggcaccgtcg acgcagagcg
     3961 cctggcgaga cgaagcgacc gccgtcggag gcgaggaaca cgcacatcgc cgcctggtcg
     4021 gccggctcgg cgacccttcc cagcgaaatc ccggtccgcc actgctcgat gctccccttg
     4081 acgatgccat cgagccgcga ggggtcaacc ttggcctgga cgtcgaccag catggtcgtg
     4141 gcggtcgagc tggggcagag cgcattgacg gtgatgccgt gcgcgccgag ctccagagtc
     4201 atgcagcgga cgagttggat gaccgccgcc ttcgacgacg aataggccgc gttgttgagg
     4261 cgcgggatgc gcgcggcgtt cgaggcgatc aggacgatcc ggccgccctg cccctctgcc
     4321 accatgtgcc gggccaccgc ctgggcgacg tggaaggcgc cgtctgtgtt gaccgagaag
     4381 ctctgcgtcc aatcgtcgaa atcggcatcg agaaaggcgc cggtccgcag gatcgccgcc
     4441 gcgttcacga gcgcgtggat ggcgccctgc gccccggcga cgaggcgatc gaccgacggg
     4501 cggtcggtga cgtcgacggt gtgggcggta tggccctcgc ccggcggttc ggccccgagc
     4561 gacatgacgt cggtggacgc catgtcggcg atcgccaccc gggccccggc cgccgccatg
     4621 gccgtggcgg tggccaagcc gatgccgccc gtgctgccgg caccgacgaa cagtgccgtc
     4681 cgcccttcga ggtcgcctgt ggtgagtgcg ttcatgcttc tctctgacgc ctgaacagcg
     4741 ggctgcaggt gatgccggcg aagaggtaga cgtcggattc ggagacggtc gcggagaagg
     4801 tcacgacctt gccgacgggg aaggtgctga tcgaatccgt catgggcgtc accaccagtt
     4861 gctgcctgcc gtccagccgc cgtcgacgct gatcgtcagg ccggtcatga agcgagcacc
     4921 gggcgatgcg aggaagagga cgacgtcggc gacatcctcc ggcgtgccga cccggccgag
     4981 cggcgtgtgc tgcctcgccg ccgcggcgcg atcgggaaac tacgccatgt aggcctcggt
     5041 gagcggtgtc gcgatgatgc cgggtgcgac ggcattgacg ttgacgccca tgggccccca
     5101 ctccatcgcc gcgctccgga tgaagcccgc tggccccgcc ggtcagcagc acgcgcccgc
     5161 cgctgaagtc gaaggcgcag cgcttccggc ccgccgccgt cccgtcagcc tgcacgcagt
     5221 gcccccttcc tgtccgccgt ggagatccag ctcgtcgtgc cgcgcgggat cacgcggcac
     5281 ggcagcgtca cccgggagtc gggcgcgccg ggctcgtcgc cgatcaccca cttcgccgcc
     5341 atggcaccca gatcgtaggt gggcaggcgc atggtggtga ggttgaagag ctgcgcatgc
     5401 cggttgtcgt cgatgccggc gacgctcacc tggcggggaa tctcgacgcc gccctgctgc
     5461 agcgtggtga gaacgccgaa tgccgcctcg tcggaggccg cgatcaccgc atcgggcagc
     5521 tcgttgcgga gcatcagcgc tgccgccgcc cggccgggct cgatgccgaa gccgcccgga
     5581 tagcggagtg aggcgtcgag cccagcctcg gtcatggccc gggcgaaccc ctcgccgcgc
     5641 ctgaggctcg tggagaggcc ggtcaggccc tcgacatagg cgatgcgccg gtggccgagc
     5701 ccgatcaggt agcgggtcag gtccatggtg accatccggt cgtcgacaga gatgacgggt
     5761 gcctcgacgt cgcgatcggt gagcatgacg aggcgcgtcg ccgagcggct ggcctccttc
     5821 gccagctcgc ggaaggcctc gttctcgggg gcctgcggga aggcaccgcc ggcgatgatg
     5881 atgccggcgg cgtggtggtc gaggaggagc cggaaatagg cgttctccac ggccgggctg
     5941 cggtcggtgt tgcagatgat ggtcaaaaag tcccgccggt aggcctggtc ctcgacgccc
     6001 cgggcgatct ccgcgaagta gctgtcggtg atgtcgccga tgaccacgcc gatcgtgttg
     6061 gtcatccggc ggacgagggc gcgggcgatc aggttcggcg cataggagag gtcggtcgcg
     6121 gcctgcagga cgcgctcgcg cgtcgccgcc gccaccgggt aggtggcacc gctcatcacc
     6181 cgcgaggcgg tcgccagcga cacgttggcg cacgcggcca cgtcggcgag cgagaccttg
     6241 ccgcccgttc cgctacgtct ggctctggac atgcgcctct cgactgttcc tgcccggcat
     6301 ctcaggccag cagctggcgg gcgatgatga cccgctggat ctcgttcgtg ccttcgaaga
     6361 tctgggtgat ccgggcgtcg cgatagagcc gctcgacctc gaagccgcgg atgtagccgc
     6421 tgccgccgaa gatctgcagg gcgtcggagg tccgcgccac cgccgcatcg ctggcgaagc
     6481 atttggcgat ggagcagcgc agcgtcgtgc cctcccccgt ttcgagggcg tcggcggcct
     6541 cgtaggtgag cgcccgggcc gcgtgcagat ccttggcgat gtcggcgagc aggaactgga
     6601 cggcctggaa atcggcgatc ggctggccga actggcgccg catcttcgcc tgcccgaccg
     6661 cggcgtcgag cgccgcctgg agaatgccga cgcacatgcc ggcgatgccg acgcgacccc
     6721 tgtcgaggca gctcatcatc atgtgaaagc cgcggctctc ctcgcccagc agggcttcgg
     6781 cggccagctc cacatccttg aacgagaccg gccccacctg cgaggagcgc tggcccatct
     6841 tgtgctcgcg ccggccgcgc gagatccccg ggagatcgcc gtcgacgagg aagatgctca
     6901 tgccacgccg gccggccgcc ttgtcggtga ccgcgaggac gaccgcgaag tcgaagttcg
     6961 ggccgttgtg gatccagatc ttctcgccgt tgagccgcca gccggtgctg tggcgctcgg
     7021 ccgtggtgcg gatgccggaa acgtccgagc ccgcctcggc ctcggtgatg gcgaaggcgc
     7081 tgaccgcttc gccggcgagg agcgcagaaa gatatttctg cttctgtctg gctgtgccgg
     7141 ccctggccag gagtgtcccg atcatctcca cattgccgag ctcgtcggcg agcccggcat
     7201 agccccgcgc cacctcctcc atggtgatcg cgtagctccg cgcgtcggcg ccggtgccgc
     7261 catccgcctc ggggagcgtg gcaccgaaga gccccacctc ggccattcgc cggtagaggg
     7321 ctgccgggaa cgcctcctcg gcatcgagcg ctgccgcagc cggacggatc tcgccatcgg
     7381 cgaagcagcg cgccgtcctg cggatcatgg catgaagctc gctgtccaag gtccgcgacc
     7441 tagaagggcg tcggatctac gaagcggcgg agcacgttgg tcgtgatcgt cgcgacgttg
     7501 ttgcgatagc cgttgtacgg cagcgagccg acataggtga tcgagccgac cgagaagacg
     7561 gccccgccct tgggcgtctc gaagaagacg aggtcggcgc ggaggatctc cgaatccggc
     7621 cggtcgggga actggcgcag catttcctcg ttcaccagcc ggaacatcgg cccgtgcccc
     7681 tctgacgagg ccagcacgag ggcatgcggc ggggtgccga gctcgatgtc cgcgcggtcg
     7741 acctcgaagc cggcggcccc gccgccgaag aagccgaaat cgccgatcag ctcgtcggcg
     7801 ccgacgccat cgaagacgaa ggcggcacgc ggatcgaagc tgccgggctg ccggcggtag
     7861 tagctgccct cgtaattgcc ctgggacgag aagccgacgc cgaccagccg gttcggcggc
     7921 cgcgcgttgc gccgccagag cccgccatag ctgccgtcga aggcgtggta gtactcgccc
     7981 ggctcggccg cccagaggcg gatcccgccc tcggcccggc gcagctcgat gacgcccggc
     8041 aggtcgggat ggacggcgac cttccagtag aagccgttgc cgccgagata catcagccgg
     8101 ccaccgccgc cgatgaaggc ctggagtgcg tcgagcgtct cgggcgtgtg gtactccggg
     8161 tgcccgccgg tgacgacgca cctgtaggca cgcaggagct cgaccccttc gttgtgcacg
     8221 tcctcgtcgg tgatcacgtc gtagggctcg ccgcaatgct cgagccagga ggtgacgtag
     8281 gtgtccgcgg cggcatggcg caggccggac cccttggggt ccgtggcggc gatgaagcgc
     8341 gggcgcatgt tgaggttcgg cctgaggcgc gagctgaagc agatgccgct gccgtcattg
     8401 tggtgattgt aggtcgaccg gccgtattcc ggatggtcct ccatgaagta cgggcaggcg
     8461 ccccactcgg cgatccgctg gcgcgttcgc gcctcgaggc cgggcgaatc gaagttgcaa
     8521 taggccatct gggtgaaggt cgagacgagg aaggccaccc tggccgtcgg cttgcccttg
     8581 ggcgggcaga cagcgaaggg aatgtggtcc tcgtggtcac cgcagcgcag ccgcatggcg
     8641 tagttgcccg aggggaagcc ctccggcggc acccacgcga agtccgtcgc ccaaccgcag
     8701 tcgtagatgt cgtcgtcgtg gaaatggatc gcggcgaact gccccggcgc ctccgtccag
     8761 cgcatgatcc gcccggccca acccgcaccg gtcatcgccc tcgcgggcag gttgacgagc
     8821 tcgccgtgca gcgcatccgg gcccacatcc tcgatctgct gggtggtcat gccgcgggcg
     8881 aagtcccact cggcgaaggt cgactcctcg ccgccggcgc caatccgcgt gaccacgggt
     8941 gcctcgagct tgccattgta gtgctcgccc accgcggccg gcccgtcgcc cgggcgcacc
     9001 tcggcggcgg cgatgaggac gggccgctcc ggcgcatccc agcggccggg cgtggcgtcg
     9061 acgctggccg agccctcggc gggatcgaag agcgatggcc gaagtgcctt gaaggctacc
     9121 cagacacgct tcttcgccgc gtcccagccg ccgctgacct cgtaccagca gcgggcgcgg
     9181 agcggcgcct cgcaggccgc ggtgaacgtg cggctgccat cgcccagcga gaacgagacg
     9241 gcaccacgct cgtcgatcgc cagcgaagcc ccccggcccg gggcaacgtc gcgccgcgtg
     9301 atcagcgcct gccggccccg ccccgggagg gtcggccaga tcagggcgcg aaagcggata
     9361 ccgccatcgc caatgccggg ggcaccgtcg atgcgcgcat aggagccgag atgcaccggc
     9421 tgaaaccgcg acggataggt gccatcaact gtggcgccga tctcgacctc gctgaagccc
     9481 gggccgtgcg gattggcgtc gccgcagagg acacgaacca gccgagcctc gtacggccgc
     9541 tcgctcgtcg agctgacctt gaagcagatt tcctcgccgc ttctgacact tgcgcgattg
     9601 gcatagccga ccagcggaat catgaacgcc ctcttctccg cgtggcagca ttcgccaccg
     9661 ccctgcagta accgtttact catgaccgcc aacgcccgtc aacgttccgg tcagcccgtt
     9721 ccggccgatt gacacaggca agctcgctgg tcagaaggtc gatagagtgg cgtcggaccc
     9781 gatctgtggg gaagaaaatg gctaccgtcc gcagaaaaaa ttttcttctc aggaggaagc
     9841 catccggtgg cagaatcggg ctgcgaaatg cccgaatcgg tcgaagctgc tgaagtcagc
     9901 aggcatgggc gaggcttgag gttgcgatgg ctaactcctc cgatccctcc atggtggtgc
     9961 tgaccgaggc ggacgtcgag gcgggccttc ggctttccgg cgagatgggc tggaaccagc
    10021 tgccggcgga ctggctgctc tatctgcggc atggccacac ggtgggcttt cgccggcccg
    10081 atggcgtggt cgtcggcact ggtgccgcca tgccgtacga gtgtggctac ggctatctgg
    10141 cgctcgtgct ggtgacggcc gactggcgtc ggcgcggcct cggcacccgg ctggtcgagc
    10201 acagcatcga ctggctcgag ggccagggca tggtgccgct tctcgatgcc accgaggcgg
    10261 cggcggaggg ctatcggcgg caggcattcg cccccgtgat cgagctggat cgctgggagg
    10321 ccgacctgcc tgcggccgag ggagcggcag ccggcgccgg ccctcatgag atcgagctgg
    10381 cgatgctggt cgggctcgac gcccaggcgt tcggcgcaac gcgaccgcat ctgctcgacg
    10441 acatacgccg tcggccgggt gtccgtggcg tggtcggtgc cgaaggccgg ggcgtggcca
    10501 tgatccgccc cgggcgccgc gcagctcaga tcggcccgct ggtggcaccg gacgatcgaa
    10561 cggccgcggc actcctggac gaggtgctgc ggggtgccgc tggtgccgtc atcatcgacg
    10621 tcccgaaggc acggacggcc ttcggcgaag ccctcgaggc caagggcttc agccggcggc
    10681 ggagcttcct gcgcatggcc cgtggccggc cgccggagcc ggcgggcccg ggtcttctct
    10741 atgcctcgac cggtgcggag tacggctgat gacgctgcag gatccactcg cgatcatccg
    10801 cgagggtgcc gccatccccg cccatccgct ggccctcgac gcggcccgac ggctcgccca
    10861 gcggcgccag cgcgcgctga cccggtacta catcgatgcc ggggccggcg ggctggcggt
    10921 gggtgtccac acgacgcagt tcgccatccg cgaggcgggg ctctacgagc cggtgctgtc
    10981 gctcgccgcc gagacggctg ccgactggac ggaccggccg ctcgccatga ttgccggtgt
    11041 cgccggccgg accggccagg cggtggccga ggcgcgcctc gcccgctcgc tcggctacca
    11101 tgccgtcctc ttcagcctcg ccgccttcaa gggggcgagc gaggacgagc tgatcgacgc
    11161 ctgcgccgcg gtcgccgccg agctgccgct cgtcggcttc tacctccagc cggcagtcgg
    11221 cggggtggag ctcggtaccg ggttctggaa gcgcttcgcc ggaatcgaca acgtcgtcgc
    11281 gatcaaggtc gcacccttcc accgctaccg cacgctcgat gtcatccggg gaatcgccgc
    11341 ggccggtgcc gaggagcgca tcaccctcta caccggtaat gacgatcaca tcctgctgga
    11401 cctgctcacc ccgttcacgg tgatgcgcga cgttgagccc gtgacgcttc gcttcaaggg
    11461 cgggctgctc ggccaatggt cggtctggac ccggacggcg gtggcactct tccaccgctg
    11521 ccgggaggcc gcccggcacg aggccgtgcc ggccgagctg ctcgccctcg acagccggat
    11581 gaccgactgc aacagcgcct tcttcgacgt cgccaacaac ttccacggct gcatcgccgg
    11641 ttgccacgag gtgctgcgcc gccaggggct cctcgacggc atctggtgcc tcgacccggc
    11701 cgaggggctg agccccggcc aggcggaaga gatcgaccgg gtctgcgctg ctcacggcga
    11761 cctcgccgat gatgccttcg tcagcgagaa ccgcgaccgc tggctcgccg ggtagcggcc
    11821 cgatacgtcg cgaccttcgt ccgcgacatc acctgtcgcc gtccgaaagc tcccgggcgc
    11881 acggcgttcg cgaccgcgaa cggcgagcgc aggcttggcc tgcctgcgaa acgccgacat
    11941 actgcggttt cgggcggcgc ttcccgggtt gtcgccacgg cggggccgat gcgacgccga
    12001 cagggacagg tcgaggagcc ggcgacttat ggagctgctg ccgagggtgc tgctcttgct
    12061 gaccgagcgc acgttccagc aggcgttcac ggcagcccac acggaacggc tcgccgggtc
    12121 gtgccgcatc gtgcgaccgg caccttgcac cagcctcgcg cgcgccgaca gcctgcagga
    12181 tgtcgagatc gtcgtcaccg gctggggcac gccacgtctg gatgaggcga cagtcgccgc
    12241 cctgccggcc cttcgcctga tcgcgcatgt cggcggcacc gtgaagccgt tggtcgatcc
    12301 tggcgttctg ggcagcagcg tccggatcac gtccgccgtg gcggccaatg cgcggccggt
    12361 cgccgaattc gccctggcat tcatcctcct ggagaacaag aatgtcgccg aatgggtgcg
    12421 gcagtaccgc gcccggcgca gccgcctcgt tttgggtccg catagcgcat ccggaacgat
    12481 gggcaatgcc ggcaagaccg tgggcgtggt cggtgcctcc cgggtcggcc gttgcctcct
    12541 gcatctgttg cgggcgttcg acctgcggcc actggtgcac gacccctacc tgtcggccgg
    12601 ggacgcatcg gccctgggag cggaaccctg ttcgctccat gacctgctgc ggcgaagcga
    12661 tgtcgtgacc ctgcacgcgc cttccctgcc cgccacccga cacatgatcg gtgcctcgga
    12721 gctggccatg atgcgtgacg ggagcctgct gatcaacacc gcgcgcggcg agatcctcga
    12781 ccacgacgcc ctcgtcgccg agctccgcga tggtcgcctg cgggcgatcc tcgacgtcac
    12841 cgatcccgag ccgctgccgg acagcagccc gtttttcgat ctgcccaacg tgatcctgac
    12901 gccgcacgtc gccggctcgc tcggggtcga gattcatcgc atgacggagc tgatcctgac
    12961 cgagatcgag cgcttcatcg gcacgggcac actggcccac gagatcaggc gagaggactg
    13021 ggcaaccatg gcatgagcat cgagcctgcc ttcctgcccg gcctctgctc cgtcaccttc
    13081 aggagcatcg acgccgagca ggtcatcgcg ctggccgtcg aagccggggc ccggggcatc
    13141 gagtggtgca gcaccgtgca cgtgccaccg ggccaccttg cgaccgcgat gcggatcgcc
    13201 gcgcagtgtc gcgacgccgg cctcgatctg ccctcctatg gcacctacgt ccgggccggc
    13261 gcggacgacg agcagcagga catgggtgct gccctcgata ccgccgcggc gctcggtgcc
    13321 ccgaatgttc gagtctgggc aggaaggcag ggttccggag ttgccgggga ggccgaccgg
    13381 gcgcgggtcg ccgatgccat ccgtgcctat gccgcgctgg cggcagccag cggcatcact
    13441 ctttccatcg aatatcaccg cgccaccctg accgacaccc tcacctcgac cctggatctg
    13501 ctcagacgga tcgatcaccc gaactgctat gcctactggc aaccggagcc cgggacgccg
    13561 ctcgagagcg ccgccgcgga gcttcgcgct ctcgggtcgg acctgtcgca cctccatgtc
    13621 ttccactggc aggccggggg tgctcgccgt ccactggagg aggccgaggc ctattggcga
    13681 cggctggtgc gagtgcccgg cagggacagc agatttgcgc tcccacgtta tgcgttctcc
    13741 gagttcgtca gaaacgacga tccggaccag tttcgtcgtg acttcaaggt gctggtgcga
    13801 ctgctcgacc ctcggatagc ggagcgtggc gatgactgat cgccgtcgcc gccgtgactt
    13861 ttccgcagcc ggggaatagc gtagaggcag ttggcgcgtg actgaccgtg ggctaggcca
    13921 tctcaatgaa gacaatgaca tcacctctcg gcgctcctgc atgacggcca ggaccgtcct
    13981 gctcggcgcg ctgctggtcc ttggcccggc cgtcgccggg tccgagcctt tcaaggaggc
    14041 gccggagctg gcggccctgg tcgccgaggg caagctgccg ccggtcgacg aacggctgcc
    14101 gacccagcct ctcgtcgtca gcccgctcag cgagatcggc acgtatggcg gcacctggcg
    14161 ctcggcgctc aagggcgcct cggacaccgg ctggctgcgt cgcaccgtcg cctatgatcc
    14221 cctggtcgcg tacagcttcg actgggacga cgtggtgccc aatgtcgccc tgtcggtcga
    14281 tgtgaacgac gacgcgtccg agttcgtctt ccaccttcgc gaaggccaca agtggtcgga
    14341 cggcacgcct ttcaccgccc acgacctcga gttcgcgatc accgacgcca ttcagattcc
    14401 cgagtatttc ggtgccagcc gcaacaccca cctgctgggc gccaaggcga ccgcggtcga
    14461 cgacctgacc ttgaagatcg agctgccgga gccgaacggg ctgtttttga agcagatggc
    14521 gtcggtgagc ggcatgtggc cactgatgta ccaggaggaa tactgctcgc agttccacat
    14581 caagtacaat cccgacgccg acgcagaagc caagtcggcc ggcctgaccg ggtggcccga
    14641 ggccctgtat ctggcttgtg acctcgacaa ctggcgcgac accgagcggc cgaccctgct
    14701 ggcctggaag ctgacttcgc catatgatgg cctgtcgcag caggtcacct tcgagcgcaa
    14761 cccgtactat ttcaaggtcg acagcgccag caatcagcta ccctacctgg acagcctgca
    14821 gatgcggcag agcagcagcg tcgacgacat ggtcctccag gcgctgaacg gcgacatcga
    14881 cttcatggac cggcacattg ccacaaccgc caacaagccg ctgttcatcg acggccaggc
    14941 gaagggcgac taccgcctct tcccgactgt ccaggccaac atgaacacct cgatcatcca
    15001 gctcaaccag aactccgaga acccggccct gaacgcgctg ttcaacgagc acgacttccg
    15061 ggtcgcgctg tcgctcgcga tcgaccgcca ggagatcatc gacgtgctgt ttgtcggcca
    15121 gggcgagccc tggcaggcgt ccccgcggcc ggaatcgagg tggcacaacg agcggctggc
    15181 caagcagcac accgagttcg atcccgaccg ggccaatgcg ctgctcgacg gcctcgggct
    15241 caccgggcgc gacgccgagg gcttccggct cggtcccgac ggcaagcgga tcgcgtttcg
    15301 cctcgacgtc accagcgacg tccagccgac cttcccggac gtggcggaga tgctggcgat
    15361 ctactggcgg gccctcggca tccagctcga cgtgcgcaag agcgagcgca gcttcgtcta
    15421 cgagaccatg cgcgccaacc ggcacgacgc acacatctgg cagggtgacg gcgggctcgg
    15481 cgacgcgatc ctcgacccgc gctactattt tccggctaat gtcgaatcgg tctacgccct
    15541 gctgtgggcg aagtccctct atgatcctgg cgccgccgac gcgctggagc caccgccggc
    15601 ggtcaggcga cagcaggacc tctatcgcga gctccagcgc tcggccgatc cggtgctgca
    15661 ggaccagctc ttcgccgaga tcctcgagat cgccgccgac gagttccgcg tgatcgggat
    15721 cagcctgccg ccgaacggct acggcatcgc caggaacgac atgggcaacg tccccgagct
    15781 gcagccccat tcctggatct acccgacacc ggggccgatg ggcgtggccc agctcttcca
    15841 caagcggggc tgacgcgtgt tccgggccag gcggcgcttg cggctcggcc gacacggggc
    15901 cggataccgc cgggccggat cgtagccggc cgatgcccat ggctcgctac ctggcgcggc
    15961 ggctgctgct gatggcggcg acaatggcgg cgctgtcgat cgtctgcttc gcgatcatcc
    16021 agttgccgcc gggcgacttc ctgagcgccc ggctcgccga gatggcgggc accggcgata
    16081 cggtcgatgt cgccaatgtg gcgtggctgc gcgcccagta cggcctcgat gagcccttca
    16141 tcgtgcaata ctggctctgg atcagcggcg tcgtcggcgg cgatctcggc ttcagcttcg
    16201 agctgcgcat gccggtcgcc gaggtgatcg gcgagcggct gggcatctcg ctgaccgtcg
    16261 agatcctggc catcatcgtg ctttgggcca tcgccgtgcc ggtgggggtc tactcggccg
    16321 tccgccagta ttccatcggc gacatggcgg cgacgctgtt cggcttcatc ggcctcgcga
    16381 tccccaattt ccttctggcc ctgctcctga tgtatctgag ctacatcgcc ttcggggtca
    16441 cgatcggcgg cctgttctcg cccgaatacg tcgacgcgcg ctggtcggcc ggccggctgc
    16501 tcgatttcct cgggcatgtc tgggcgcccg ttctggtcat cgcgaccggt ggtgcggccc
    16561 agatcatccg catcctgcgc gccaacctcc tggacgagct gagccggccc tatgtcgaca
    16621 ccgcccgcgc caagggcatg gccgagtggc ggctgatcct gcgctacccg gtgcgggtcg
    16681 ccatgaaccc gctggtcagc acggtcggct ggctgctgcc gaccatcatc tcgagctcga
    16741 tcgtgaccgg aatcgtcctc aacctgccga cgctctcacc gatcctgctg cgttcgctgc
    16801 tggcgcagga catgcacctg gccggtggcc tgatcctgct gatcggcgtg ctgaccctgc
    16861 tcgggacact gctctccgat atcctgctgg cgtggctgga tccacggatc cgcctcggct
    16921 gacctcgatg ctcacccgtt ccggcgatgc agcgccgcga ggcccacgcc gcccgaccgg
    16981 caccgagtcg ccgtgggcgc tggtctgggg ccggtttcgc caccatcgtc tcgccctggt
    17041 cagcggcgtg atcctggcgc tcctctatct ctcggcgatc ttcgccgagt tcctggcgcc
    17101 gggcgacccg caagcctaca acgcccgcta caccttcgcg ccgccgcaat cgatccatct
    17161 gctcgaccgc gacgaggacg ggagcctggt cttccgcccc tacgtccacg gctatgcggt
    17221 gaccgtcgac ccggaggcga tgcggcggac cttcgcgatc gatccggaca aaaagcaccc
    17281 gctgcggctg ctcgggaggt ccgagccgta tctgctgatg ggcttcatcc cgatgcagcg
    17341 aaagctcctc accatcgagg gccagggcac cccgctcctc ctgctcggcg ccgatcggct
    17401 cgggcgcgac gtgctcagcc ggctgatcat cggcacccgc gtctcgctct cggtcggcct
    17461 ggtcggcgtt gtcctcagcc tcgccatcgg cctcgtcgtg ggcggcctgt ccggctacca
    17521 tggcgggctc ctcgacctgg tcgtgcagcg ggtcatcgag ttcctgcgct cggtgccgac
    17581 gatacccttg tggatggggc tcgccgcagc actgccgcgc gactggccac cgctgaccat
    17641 ctatttcgcg atcaccgtca tcctctcgct ggtcggctgg accgagctcg cccgggtcgt
    17701 gcgcggccgg ttcctggcgc tcaagacgga ggactacgtg accgcggcgc ggctcgatgg
    17761 cgccagcgac ctttccctgg tcttccgcca catgatgccg gctttctcga gccacatcat
    17821 cgccgccgcg agcctcgcca taccggcgat gatcctggcc gagaccagcc tcagctttct
    17881 cggcctcggc ctgcaggccc cgatcatcag ctggggggtg ctgctgcagg aagcgcagaa
    17941 tctccgctcg gtggcgacgg caccctggct gctctggccg gccgcggcga tgatcgtcgc
    18001 ggtcctggcc atgaactttc tcggcgacgg cctgcgtgac gcggccgacc cgtacgccac
    18061 atgacagccc cgcccgggcc cgccccggcc gccgaagtcc tggccattgt cgatctcgag
    18121 acccacttcc cgctgaaccg gggcaccctc aaggcggtcg aaggggtctc cctgacgttg
    18181 cgcgccggcg agacgctctg cgtcgtcggc gagagtggca gcggcaagag tgtcaccgcg
    18241 cgctcgatcc tgaacatcgt caagccgggc aggatcgtcg gcggccacat ctggtttcgg
    18301 cccgatgccg tgattggggc cgggaacggg aacgggcccg cgatcgatct ctgcgccctc
    18361 gaccccgacg gcccccggct gcgagcgatc cgtggtgcgc ggatcgccat gatcttccag
    18421 gagccgatga gcgccttgag cccgatgcac acgatcggcc agcagatcat cgaggcggtc
    18481 cgcctgcacc gcgcgatgga caagcaggag gcacgcgcga gggccatcga gctgctccgc
    18541 caggtccagt tccgcgagcc cgagacggcc atcgaccgct accccttcca gctcagtggc
    18601 ggcatgcgcc agcgggcgct gatcgcgatg gcgctgtcct gccagccgca ggtgctcatc
    18661 gccgacgagc cgaccaccgc cctcgatgtc accacccagg ccgagatcct cagcctgatc
    18721 aggtcgctgc agcaacgcta tggcatggcg gtgctgctgg tcacgcacga catgggcgtg
    18781 gtcgccgaga tggcggaccg ggtcgcggtg atgcagggcg gccgggtgat cgagcacggc
    18841 ccggtcgacg cggtcttccg ggcgccgcga gaggcgcaca cgcggcgcct gatcgaggcg
    18901 gcccggctgc tcgagcggcg cggtgaccgc gctgccggca cgcctgccgg cggcccgggg
    18961 caagcgccga tcctgtccgt gcgcggtctt tgcctcgcgt ttcgcgggac caccggcttc
    19021 ctgcgccgcc gggcgaccgc cgtccatgcg ctgcgcgacg tgtcgttcga cgtggcaccg
    19081 ggcgaggcgc tcggcatcgt cggcgagagc ggctccggca agacgacact ggcgcgcgcc
    19141 ctgctcggca ttcaccgggt ggatgcgggc caggcgcggt ttcgtcgggc cgacggctcg
    19201 ctggtcgatc tctgcgcccg gcagggctat gccgaccggt cgctctatgc cgagatccgc
    19261 atgatcttcc aggacccgtt cgcggcactc aacccgcgcc agacggtcga gcagatcatc
    19321 gccgagccgc tgcgcattca tcggctctgc cagcgcagcg ggatcggcga ccgggtcgcg
    19381 gcgctgctgc gcgacgtcga tctttcggcc gacgtcatgg accgctaccc gcacgccttc
    19441 agcggcggcc agcggcagcg gatcggcatc gcccgggcca tggccacgaa cccgcggctg
    19501 gtcgtggccg acgagccgac ctcggcactc gacggctcgc tcagggcgca gatcctcgac
    19561 ctgctgctcg agctccgcaa ccggctcggc ctcagcatca tcctgatcag ccacgacatc
    19621 ggcgtggtgc ggtatttctg cgagcgcatg gcggtgatgc gccagggcga gctcgtcgag
    19681 atcggtgcgt cggaccagat ctgcgacgcg ccgcgacatc cctacaccgc cgagctcctg
    19741 gccgccgtgc cgcgcgccgg cccccgccac gccaggctgc gcgatccgga cgcggccggc
    19801 cccgactgaa ctaaaggaat gataacctat ttctgtgctg cgtgcaagcg tggcgcgggc
    19861 cggcccgggt tggttcggca attcgtgact tctccagctc gacgccggcg catcggccgt
    19921 cgaaaacgct caaaaaatgt tatggcacaa taggttgaag gcgcctgggt tcgttggtcc
    19981 tggcgtgggt tcgttcgtcg aaaacccggg ttcgctcgtc cgaatcccgg gttcgatcgt
    20041 cgaatccgcg ggttcgttcg cggcggcgtg ttgcagaatc cgcgacaatt ccgggcgaat
    20101 gtcgcaccag acgcgccatc acgaatcgtt atgtcgcgga atctgcaaca ttccggcggg
    20161 atgtgtcgcg aaatacgcga caacccgagc cggaatgttg catcggatgc accaacccga
    20221 gcagctccgg gggacggagg acgtcggaag tcgcgcacgg cccggtttcc gaagccgagg
    20281 tataagcatt cgtgatgccg ccgaggtcgg ggacttccct gtcgcctatc ccggtgccat
    20341 ggccgggtcg ctgaccggca ggccggcgag gagtgcccga gcctcgtcgg cccggccgat
    20401 gcgcgtgtag agctcggcca gggaggtcgc cgcgcgccgc tcccaggcca gcgccccctg
    20461 tccccgggcg atctccaggg cgcgccgcag atcgacctcg gcggcgacgc tcgccgggtc
    20521 gcgctggccg aggatcacgc cgcgcacgcg atggatctcg gcgttgcacc aggcctgccc
    20581 gctgacctcg ctttcggcga gcgccgcatc gattcgcgcc agcgcctcgc cgcttcgccc
    20641 ggctctcgcg agcgttccgg cctcgagggt caaaagatag gggagcatcg gccgattgcc
    20701 gagcttctcg agctgcgcca gggcgcccgc catctcgtcc gcccccgttt cccgatcgcc
    20761 ggcggccgcc agcgcccagc ccctgagggc cttcgcctgc gcctggaaat gggggatccg
    20821 ctggccctcg cacagctcga tcgcgctttc cgcgaaccgc ctggtcgcgt cgacatcgtc
    20881 caggagctgg tgcaggaccg ccgcatggac cgccgtccag gcccggctgt gcgccgggac
    20941 cgtcggcccg gcgagcgcca gggcctcggc catggtggcc tgggccggcg ccgtctcgcc
    21001 cgccagccat tccgcccagg acagaaagag gcgggtcgtc acgccggggc tcgccgcata
    21061 gcgcgccgcg aggtcgtgct ggaaggcggt atcccgcgcc gccgcgcggc gcagcagcag
    21121 cctcgcatcc gccaatcgcc cgagccagaa cgagaccacg ccgacggccc agtcgctgat
    21181 caggcgggtc tcggccgagc ggtcctgctc ggctgcggcc tgcagctcgc gcgccagccg
    21241 atcggcagcc gcgtactcgc cccgcaccag atggaagcgc cacaggccga agcgcgcgac
    21301 caggcccttc tggcgctcgc ccagctcctc gcagagcgcc agggcgcgcc agtagcactc
    21361 gccgacggcc tcggcaccga agccgcgcag ggccagcagc acgaagccca gcttgagctg
    21421 caggtccacc tcctcgcggg ggcgcgccgc ctcgggcagc tcgggcagca gtcgcaaggc
    21481 gctcgccaga tggtcgaccg cctcggcatt ggcggcgcgc gccgcggccc gttcgccggc
    21541 gatgcgccag taggcgatgg ccggcacggg cagccgggcc tcgccgaaat ggtgggcgag
    21601 aagctcgggc gccatctcgg cgagctccgg gaagcgctcg accaggaccc gggcgacgcg
    21661 gccatggagc tgctggcgcc gggacttgag cagggactga taggccacat cctggacgag
    21721 ggcgtgcttg aagctgtagc tccgggccag cggcgagccg tgctggaaga ccagctcgga
    21781 ggcggtcagc cggtcgaggg cggcggcgat ctccggctcg gggcggccga gcgccgccgc
    21841 cacgagctcg aggctgaact cccgcccgat cacggcgccg acctgggcga tctccttgac
    21901 cgaggcgagc cggtcgagcc gcgccatgag cgaatcgcgc aacgtcgccg gcacggccag
    21961 cgatggcgac ccggccagcc cgaagcggtc gtcggcagcg tccggcaggc tcgattcgag
    22021 caccgtcttg gtcagctcct cgaggaacag cggcaccccg tcggtgcgcc cgacgatctc
    22081 ctcgagcagc ctcgcggaaa tggtgccgcc cggggccagg ccctcgacca tcgccgcgct
    22141 gtgccgcagg ctcaggcgtt gcagcggcag ccgggtgaca tggggctgcc cgccccaggg
    22201 cgactcgatg tcgtcgcgca gcgtgacgac cagcagcacc ggcagcgtca cgaggcgctc
    22261 gaccagcagg tcgagcagct ccctcgtcgt cgggtcgatc cagtgcatgt cctcgagcac
    22321 gatcaggacg ggccgccgtg cggcgagccc gtcgagctgc tcgccgagga cctcgaaggt
    22381 ctgctccttg cgccgctgcg gcgacgggtc caggggcggg tagcgcccgc cggtcggcag
    22441 ggtcagggcc tgggccatga gcgccgcagc ctcggccggc cgcgctgtcg cccggccgag
    22501 cagagcctcg agcttgtcca gccgcacgct gtcgctgtcg tcgcgctcga agccggcggc
    22561 gcgctgcacc tgctcgagaa ccggatggag cgcgctgctg acatggaatg gcgagcagaa
    22621 gtagcgaagg cagacatggc tctcggcgtc gagctgctgc cgcaacgctt tcgcgatgcg
    22681 cgacttgccg atcccgggct cgcccgagag cagcaccacc tgaccccggc cctgcttcgc
    22741 gtcccgccag cgggcgagca ggaggtcgag ctcgtcctcg cggccgacca gcggcgtcag
    22801 gcggcccccc tgcaacgcct cgaagcgccc caccgtcggg ctctcgtcca ggacacgcca
    22861 ggctggaacg ggtgcgatga agcccttgag gtcgtgcacg cccagatcgg cgagctcgaa
    22921 ccggccaccg acgagccgcc gcgtgctgcg cgagatgacg atggtgttcg gcccggcgag
    22981 agactgcagg cgagccgcca ggttcagcgc ctcgccgacg accgaatcct cccgcacggc
    23041 gcctgcgccg atcggcctgc ccaccatgac gagcccggtg gcgatgccga tccggacggc
    23101 gagcggctcg ccgtcggcga ggatgggtgc aaacgccgtc cgcatctcga ggccgcaccg
    23161 gatcgcccgc tcggcgtcat cctcgcgcgc ctctggatag ccgaaataga gcaggatgcc
    23221 atcgcccaga tactggccga catgcccgcc ccagcgcccc accagctcgg tgcatcgatg
    23281 ccggtaggcg ccgatcacct cgctcatgtc ctcgggatcg agtcgtgccg aaagccgggt
    23341 cgagccgacg acatcgcaga acatcacggt gagctggcgg cgttcggcct ccggcggcat
    23401 cgcccggtgc gcggcttcgg cgcgaaggcc ggcagcggcc tcgccaccgg cggcgagggc
    23461 ggcgatcgcc ttgagcagct tcttgcgtgg gcccagcggg atgccgagcc cggcgaggtc
    23521 gccatcggtc agctcgggca aaatctcggg ctcgatctcg cctgcccgga acgcctcggc
    23581 gtagcgctcc agcccgagtt cacgcagcca gccggcaatc tccaagtcac ccccccgatg
    23641 gcaccccgga tgcagagaga aagccgcccg cgcaaccttc tcggcccatc ctccggcagc
    23701 ctcggcagcc cggcccgagg ccggccatca gcagtcgacg aggtccggct cgggctgttg
    23761 catcgtcttt ccttgcgccc gataatggtg cccggcacct cggcaggcaa cacctcgtca
    23821 gaaaggttgt cgatcgggca gtcgatttgt ccaggcccgt ccctgtcggg gtcgcgattt
    23881 gcggccggcg ggctgcccga accgcaaccg ccattcccgg cggctcggag ctgccgaaag
    23941 gacctcggat gccggcccga cttcatccct acctgttcct gacccctggg cgcctcgacc
    24001 gtatccgcca ggggctggat gcctcgggca gcggccatga cccgacggtc cagaagtggg
    24061 tcgacaagat gcgtcgacgg gcccgcgaga tcatcctgcc gaagccgatc ctgacaccgg
    24121 actggatagc ggcgggcgaa ggtgcgacgg cgcccgtcat gctctacacg gcgcgcgccg
    24181 ccgtctcgcg gatcacgatc ctcggcctgc tctggcatat cgacaaggac ggtggcgaag
    24241 ccgaccgcaa cggctcgggg ctggaggccc aggaatggct ggcccgggcg tgccgggagc
    24301 tcttggccat cgcgtcgttt cccgactggc agaagccctc gttgccaccc gaggtgtttc
    24361 tctcggtggc cgagatgtgc gctgccgccg ccatcggcct ggactggctc tggcagcatc
    24421 tcaacgtcga cgagcggcgc cgggtccagg acgcgatcgt caggaacgcc ctccagccgg
    24481 ccctcgagca atacaccatc gggcgcgcca ccaaccgcgg ctggacgacc gagccctaca
    24541 actggaacat cgtctgcaac acgggcatgg ttctgggagc gctgatggtg cgcgaccggg
    24601 atccggcgct ggcggaagag gtgatgacga cggcgctccg gtccgtccag accgggttcg
    24661 cgggctactg gaacgatggc ggctggcccg aaggcccggg ctactggtcc tacgccacgc
    24721 gctatgccgc caagctgctc gatgcgctcc ggggcctcga catcgacgac cgggatctct
    24781 cctgccgtga cgggcttgag cggtcgggcg agttcgtgct gcactgcctg ggcccgaccg
    24841 gcatgacctt caacttcgcc gacagcgagc cgcgggtttc gctgatggcg atgtcatggc
    24901 tcggcgagcg cttcggccgt gacaccgaca gctggatcgc cagccacaac ccgaacggct
    24961 cgcgcctggc gctgcagctg ctctggcgcg ccggcccggg agcgccgccc gaatcgggca
    25021 ggacgccgcg cgacgccgcc ttcgcctcga tccacggcgc cttcttccgc agcgcctgga
    25081 acgacccgga cgcgctctgg gtcggcttca agggcggcga cgcccgctgg catcacccgc
    25141 agctcgacgc cgggaccttc gtcctcgatg cgcatggcct gcgctgggcg gtcgatctcg
    25201 gcccttcgct ctatgccaac cccggctatt tcatccgcga gacgcgatcg cgctactatc
    25261 gcgccagcac ccagggacac aacacgctgc ggatcggccc gcatcagcag aacgacgagg
    25321 cggtggcacc cctgctggcc tcctggtgca aggatcatga cgacctggcc ggtgcgatcg
    25381 tcgatttgtc gacggcctac aacctgccgg ccggcgagct gctgcgcggc gtgttcctgc
    25441 tcgagcgccg gcaggtgctg atccaggacg atttccgcaa cccgcccggc gccgaggtcg
    25501 tctggtcgat gcatacgccg gcgacggtga cgatcgaccc ggagcggccg ggcgtggccg
    25561 tgctgagcca ggagaggacg aacgaggcgg cgccggacgg caccccagag acctgctatc
    25621 tgagcctgcg catcctggcg ccccaaggcg ccggcttcag ccagaagtcg gcctcctacg
    25681 cggggggcac cgagtatccc gaggccagca tcaaccaggg catcaccaag ctccagatcg
    25741 tgctgccgtt cgaccgcgac gccccgctcg agcgcatcgc tgtcctgatc acgccggaga
    25801 tggctccagg gaaggcgccg agcctccgac cgctcgacga ctggcagggc gactggcagg
    25861 ccgagccgca gccgcagcgc cattggcagg acgacgtggc gctccacgtc ctcgaaggcg
    25921 tcgagcgccg gaggaacggg cggggcctct gacgccctga tgacccggcc cggcnnnnnn
    25981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtcgag cgccggagga acgggcgggg
    26101 cctctgacgc cctgatgacc cggcccggcg agcgtcaccc gcccgtaggg tccgagcgtg
    26161 gccgggccgg cagcgctgcc gcgccggcca tctcctcgcc gcgatggcac gcaacgaggt
    26221 ggccccggcc gaccggcagc agcacgggct cgctctcggc gcatctggcg atggcgagtg
    26281 ggcaccgtgt gcgaaagcgg cagccgctcg gcaggttcac caggctcgcg atctcgccgc
    26341 gcaaggccgc cggccggccg cgctggcgcg ggtccggggt cggcgacgag gcgagcaggg
    26401 cggcggagta gggatggcgc gggccggcga agaaggtcga tgcaggcgcg gtctcgacga
    26461 tcctgcccag atacatgacg gcgacgctgt cgctcatgtg ctcgaccagg cccagatcgt
    26521 gggagatgaa gagataggcc agcccccggc tcgcctggag gtcgagcagc aggttgacga
    26581 tctgcgagcg gatcgagacg tcgagggccg agaccggctc gtcgcagatc accagggccg
    26641 gctccacggc gagggcgcgg gcgatgccga tccgctgccg ctggccgccc gagagctcat
    26701 gggggaaccg ccgcccgaca tcggcggaaa ggcccaccga ttcgatcagc ccggcgaccc
    26761 gggcggcaat ctccccggcc ttgcagatcc gatgcacctt gaacggctcg ccgaggatcg
    26821 cgtcgacccg cagatgcggg gtcagcgagg cataggggtc ctggaagacc atctgcacgt
    26881 tgcgccgcag ccggttcagg ctggcccgcg atgcgccatc gacccgctcg ccctgcagcc
    26941 ggacctcgcc ccggtccggc cgctcgagct ggaggatcag cttggccgtg gtcgatttcc
    27001 cgcaacccga ctcgccgacc agcgccatcg tggtgccggc cgggatcgtg aacgagaccc
    27061 cgtcgacggc cggaaccctg gtccggggcc ggccagggag cccgcccccg aggctgtagt
    27121 gcctgaccag gtcgtgcacg gaaagcactt cggcggtggt gctggcggcc gcggtcacga
    27181 ggcggcgccc acggcagcgg cggccacagc cggcagctcg cgccagcgat ggcagcggac
    27241 ccagcgctcg tcggcgaccg gctcgagggc cgggagcgcc gcgtcgcaga tgccggcgag
    27301 cgcgtgcggg catcgattga agaagcggca gccggccggc ggccgcgcca ggtccggcac
    27361 ggccccggcg atcgtggcga ggtgcgactt gctggcgctg gcgccgcgcc gcggcagggt
    27421 ttcgaggagg ccccgggtgt agggatgcag cggcgcgtcg aacaggtcca ggacgggtgc
    27481 cagctcgacg atgcagcccg catacatgac ggcgacgcgg tgcgcggtct gggcgaccac
    27541 cgcgaggtcg tgggtgatga ggatcaccgt catctgctct tcggactgca gccggacgaa
    27601 gagctcgagg atctgcgcct ggatggtgac gtcgagggcg gtggtcggct cgtcggcgat
    27661 gagcagcttc accgcaccgc agatgagcgc catggcgatc atcacccgct ggcgcatgcc
    27721 gcccgagagc tcgtgcgggt actgggcgag gcgctgcccg gcgaactgga tgccgacctg
    27781 ctccatgacc tcgagggcgc gggcacggcg ctggctgcgc gacatttcgg gccggtgcgc
    27841 gcgcagcacc tcggtgagct ggtcgccgat gcgtagtgtc gggttgaggc tggtcatcgg
    27901 ctcctggggc acgaccgcga tcgcgtcgcc gcgaatccgc cgccgttcgt ccggcgagag
    27961 gcggacgagg tcgacgccgt cgaacaggat ggcaccaccg acggtccggc ccggcggcgg
    28021 cacgaggccg acgatcgagc gggcgagcat cgacttgccg cagcccgatt cgcccaccac
    28081 gccgagaatc tcgccccgcg tgaccgagag gctgagccga tcgacgaccg cgaacgagcc
    28141 gtgcggggag ggaaagcgca cctccacccc ctccagttgc agcaccggct cgcctgcgcc
    28201 gggggccacg ccctggcggc tcacgggccg accttgccct gcagccgcgg atcgacgaga
    28261 tcgcgcagcc cgtcgccgat caggttcagg ctgagcaccg tgatggtgat catcacgccg
    28321 ggggcgatca tgatccaggg cgcggtccgg atgaactcgc gggcggcgct gatcatgttg
    28381 ccccagctcg ggctcggcgg cggcggcccg aggccgagga agctgaggcc ggcctcgacc
    28441 aggatcgccc aggcgaagcc gaaggtgagc tggaccatca ccgggccgaa ggacttgggc
    28501 aggatgtgca ccacgaggag gcgcatgtcg ccgacgccga cggcgcgcgc cgcctggacg
    28561 aagtcggccg accggaactc gatgatcgag gcccggacgg tgcggatgac gcgcgggacg
    28621 tagaggacga ccaccgccag cacgacgttg agctccgacg gcccgagggc cgccaggatc
    28681 atgatggcga ggacgaagcc cgggaagacg aggagcgcgt ccgagaagcg gccgatgatg
    28741 gaatcggcga gccggtactg gccggagagg gcgccgagcg cgatgcccag cacggcggtc
    28801 agcagggtcg cggcgaaggc gacgcgcagc gagatgtggg cgccgatgat gaggcggtcg
    28861 agcacctccc ggccgaactc gtcggtgccg agccagtgct cggcgctcgg cggcagcagc
    28921 ttctgcgcca cgtcgatgcc gagcgggtcg atctcgacca gaagcgggac gaagagcgag
    28981 gcgaggacga cgagcgccag gaccgtcccg ccgctgagca gcatcggccc ggcgcgccgg
    29041 atggcccgca gcgccgatct ggcggtcgac ggtgttgtcg cctcggcggc agcgggcgtc
    29101 atcacgcgta gcggatcctc gggttgaacc aggcgtaggc gagatcgacc atgagattga
    29161 tcacgagggc gaccagcgtc aggtagaaga tgccgccctc gagcgtcggg tagtcgcggc
    29221 ggacggctgc cgtggtgatc agctcgccga ggccgggcag cgcgaacacg ttctcgatga
    29281 tcaccgagcc gccgaggccg agcgcgaaga cgaggccgat gacggtgacg atctggacca
    29341 tggcgttgcg gagcgcgtgg cggaggatga cggtgcgctc ccgcaggccc ttggtccgcg
    29401 ccatcgccac gtattccatg cccaagacct cgatcatggt cgagcgggtc atgcgcacga
    29461 cgaggccgat ctgcgagacg ctgagcgcaa ggacaggcag cacgagacgg ctgagccagc
    29521 tccagaagcc gaactccatc ggccggtagc cggaagccgg cacccactgc aggctcacgg
    29581 cgaagaccag catgagcatg aagccgagcc agaagggcgg cagggccatg ccgaggctgg
    29641 agagcacgtt gccgaagtag tcgaggatgc cgccgcgccg catcgccgag aggatgccga
    29701 gcgggatgcc gaccgccacg gtcacgagga gggccatgaa ggtgagcgcg aaggtgatcg
    29761 ggaaccggtc caggatcacc tcggccacgg gtgccgagaa gaagagggac tggccgaagt
    29821 cgccggtgac cacccgcggc agccattcga gatactgctc gaggatcggc cggtcgaggc
    29881 cgagctgctt ggtgatcgcg tcgatctgtg cggcgtcgac cgactggttg ccgatcagcg
    29941 ccgtgatcgg gctgcccggc accagccgcg tgatcacgaa gatcagcgtc agggcgatga
    30001 agatggtcgg cacgaattcc aggatgcggc gcagggtgta gaggagcatc gcggcctcct
    30061 ggcccatggc ggtgcaagcc ccgccatggg atgacaacgg agatggtcgc tcagtcggcc
    30121 aggtagatgc cgtgcagcgt catgttgccc agcgcgaggt tgtcgtaagg gtcgaccacc
    30181 ctgccctgct tggcgaccag ccggtagacg tagccgagat tgaccatcgc gacctgctcg
    30241 tagaacgccc gctgcagctc ctcgccgagc gccttgcgct tgtcgaaatc ggtctcggcg
    30301 gcgagcctgg cgaagatctc ctcggtccgg tcgctgatga agccgacctc gggtgacggg
    30361 aaggtgccgt tgaactccgc gtgccacagg gacggatcga cccagtagcc gccggacgtg
    30421 tagatgttga gcttcgccgg gtcgcggcga ttggcgaccc aggtggcgag gtcgtagctg
    30481 acgatctcga ggttgagccc ggcccgcttg gcctgctccg cgatggcgac ggccagccgg
    30541 ttctgcaggg agttcgtcgc gatgacctgg atggtgatcg gctcgccgtc gtagttcgcg
    30601 gcggcgaggt attccttcgc cttctcgacg tcgcccgggg tgtagagatc ggacccggca
    30661 tcggtgttgt aggcgctctc gggcgcgtag atgctcgggt tggtcagcgc gaaatccggg
    30721 ttggacacgg ccacggcggc gatctcggcg gggtcgatca gcgactggat ggccttgcgg
    30781 atgttgagct cggcggtcgg cccgaccttg tgattgaaca tcatgtagag gagcacgccc
    30841 gggccgttct tgacggcgtc gagatcggga ttctccgcga gccgcgtggc ttcgccgtcg
    30901 ggcacctcgg cgatgacgtc gtattcgccg gattcgaggc cggcgacgcg ggtggtcgat
    30961 tccggcacga tccagaagat gatctcgtcg agattggcga tcttcttgcc ggtctggtag
    31021 ttcggcgcgt cgtcgcgggc gacgtagtcc tcgaagcggg cgagccgcgc gtactggtcc
    31081 tccttgtact cgacgaagcg gtaggggccg gtgccgacca cctcggtgag ctggcccggc
    31141 gtggtcgatg cctcggcgat cgccgccggc atgatgaccg ccttgttctc gtcggagccg
    31201 agcaggagca gcagatagcg gcccatgggc tcgctgaagc tcatgaccac ggtgagatcg
    31261 tcgggggtct cgatggaggt gagcgtattg agacccgacc ctttcgggcc gacggtgcgc
    31321 cagcgctcca gcgacgcctt gacgtcggcc gaggtcagcg tgtcgccatt gtggaagagg
    31381 acgccgggac ggagctcgaa ggtccaggtc ctgccgccgt ccgaggcctc cgcactcgcc
    31441 gccagctcgg gctccgcatt gaggtcggag ccgagcccga acaggccctc gtagacatgc
    31501 cccatgacgt gggggagcgg gtggccgacg gtggactgcc agtcgagcgt cggcagcggg
    31561 atgtggaggc cgacattcag cgtgccgccc tgcaccggct cgtccgcggc cagcgcggtc
    31621 cggaccagac ggccggcctc gaggtgcccc gcttcgagat gcaacgacgt ggcggcgagg
    31681 ccgccgacga ccccaacgag cacagtgcgt cgacgcagca tcgtgtcagc tcccccttgc
    31741 gcgcccgttc gggctgtttg gtagatcgtt ataccaagcg tatcggaaac cccggcaccc
    31801 cacaagccgg aatcaagccg ccagatcgcc ggcgcgcatc gcctgcgccg cccatgcctc
    31861 gtcgaggacg acgccgaggc cggggcctct gtcgagcgaa gcccggccac cggcgaacgc
    31921 cggcccgccg gtgaggccgg cctcgacttc gccccggtgc ggagcgaggc agatcgggta
    31981 gctgccgtgg atggtggcgg ccagatggcc cagcgccgcc cgcccgactg cgctccggca
    32041 gggccccgac acgacgacgc cgatggaagc ggcctcgcag atattggcga tgcgccgggc
    32101 gttgcgcagc ccgccgaccc gccagggatc gaggacgatg cggtcggccg ccgcgtgccg
    32161 cacgacctgg ctcatggcct cgggcgaggt gatcgactcc tcgagcacga tcggtatcgg
    32221 cactttttgg cgcagccggc cgtggcccgc gaaatcgagc cggtgcagcg gctggaccag
    32281 agccaggttc aggtggaact gccgggcgag cagcccctcc accagcatgc tggcccgctg
    32341 ctcgttgccc agcgactgcc gggcgacgat atcgacgaag ggcacgctgc cgacgctgtc
    32401 gagcacggca ccgatcgccg ccgccgagcg cccgggcagg gccagcctga gacccgcatg
    32461 gccgtcccgg atggcggccc gggcggtgcc caagggatct ggatcaccgg cgtcgatctc
    32521 gcgaaagagc tcgagatcct cgcgatagcc gccgcccagg aggtcgtgca gcgggcagtc
    32581 gcgagcccgg ccgacgaggt ccagggccgc catctcgagg gccgcgcggg cgggcgggca
    32641 gccgggcccg ccggcgggct cggcagcgtc gagccgctgc tcgaggacgt tggcgttgcc
    32701 gcacccggca ccttggatat gggtgccgta gcgttcgagc cagcgcgaaa cggtggcgcg
    32761 tgacatggtc ggaaaggcct cgtcggtcgc cacctcgccg agcccggcca gccccgatgc
    32821 cgcccggagt cgcacgagga tgccgccgcc aaagggcatg aggtcgatcc ggtcgatctt
    32881 catcagtggt gtcccgcgtt tagggagcca ggctccggcg ccagggcatc ggcacggccg
    32941 gcgcgcagca cgaggcccgg cgccgggcgc ggtgccgcga cggactcgcg ctggatgagc
    33001 tccgacttga gcacgatctc ccgggtctgc ccggccggcg aagcccgctt ctcgatggcg
    33061 gcgaccagcg cttcgaaggc cagccggccc atgtagaacc gttcctcgcg caggcaggac
    33121 atcgccggcg tgaagaaggc ggcccagtcc gggtcgtcga aggcgatcag gctgaggccc
    33181 tggggcagcg ccacgccgag atccttgagg cagcgcagcg caccgccggt catcagctcc
    33241 gagcagacca ggatggcggt gggcggcagc ggctgcagca gcaggtgtcg cgtcgccgtg
    33301 tagccgccgg cctggcggaa gtcgccatag gcgatcaggc tttcgtcggc cgcgatcccg
    33361 agctgctcca gcgcgtcgag gtagccgccg aggcgggcac ggccggtctc gaaggcctgc
    33421 gggccggcga cgatgccgat gcgcacatga ccgaagccgg cgaggtggcg cacggcgtcg
    33481 gttgcagcgc tccggtcgtc ggaggtgatg tgcacgagcc tgccgccctc gatccgggcg
    33541 ttgacgacga cggccgggaa gcccgaggcg ccgagcttgc ggaagacctg gcgattggcc
    33601 gccaggggcg aggtgatgat gccgtcgaca ccacgctcgc gcatctcggc caggtactcg
    33661 acctcccgct tcgggtcgtc attggtgttg cagagaatga catgatagcc ggcctggcgg
    33721 gcagcctcct cgacgccctg gacgaagagg gtccagtggt agctggcgat ctggctgacc
    33781 agcacgccga ccgtgttggt gcgccgcagg cggagcgagc gcgccacgcg gttcgcccgg
    33841 tagcccaggc tgtgcgcggc cgcctcgacc ttcagccggg tggcgtcgct gtaggagccg
    33901 tacttgctca agacccggct cgccatgccg accgacacgc cggcagcagc ggcgacatcc
    33961 ttcagggtga cagagctcct ggtcatgcac gggcgccctc ggttcgcggc gtcggtcggt
    34021 gagcgcgtcg gcatgcttag ggtttgggct atgaagcgtc aatgtatatc gttacactcg
    34081 aacccggtcc cagctcgccg aggcatcgga tgaagatcac cgccatcgac ctcgtccctc
    34141 tccgcctcga gatgctgagc gctgccgtgc ggacgggccc gaacgtcgat ccggcggacc
    34201 agatggacct gccgccggag gagatcgcca agctccgcta cgccccgccc gagaccgtgc
    34261 tggtgcggat caagacggat gccggcatcg aggggctcgg cgatggtgcc acgctgccgc
    34321 actatctcgg acacgggatc ggcagcatgc tggactggct cggccgcttc cggcaggtgc
    34381 tcgcgggcag cgacccgctc aacatcgcgg ccgtgcaccg gcggatggag agcgttgcca
    34441 gcatcggcgt gccgggctgc cggccggccc aggcggccat cgacatggcc atccacgaca
    34501 tcgtcgccag ggcgcatggc tgcccggtct acgagctgct gggcggggcc ttccgcaccg
    34561 agctcgagct gcagacgcag atgcacggct tctcggtcga gcagctcctc ggcgtctgcc
    34621 ggcactatct cgaccagggg ttcaccgggc tgaagctgaa gatcgccggc aagctgcgcc
    34681 gcgagggcta cgccaaggcg ctggtcgacg aggaggcgga gaagatcgcc cgggtcgtcg
    34741 ccagcctgcc ggagagcgtg cagatcgacg tggacgccaa ccaggggctg atgaacgcca
    34801 aggttgcgat cggcctcttc gagcatgtcc ggcggatcgc cccgcatccg aacatgtcga
    34861 tcgagcagcc cttgcatcac ctcgatctcg agggccacgc cctgatccgg cgcatgctgc
    34921 cctatccggt catcctcgac gaggcggtca cctcgcccgc cgcgatgctg cagatcgtgc
    34981 gcgcggaggc ggccgatcgc atcgtcctca agcccaacag ggtgggcggc ctctggccgg
    35041 cgcagaagat catcgacatc tgcgaggcgc acggcatcgg cgtcagcctc gacacgatgc
    35101 cgttcacggt gctgggcgac accatgctct gccatctagg cgccaccatc cgcacccact
    35161 acccgctcga tgcggagggc cacacgttct tcagggagac cccgttcgtg ggcggcgtca
    35221 cgctgcagga cgggcgcgcg cggctgtccg ccgagccggg cttcggcatc acggtcgacg
    35281 aggaccggtt gctggcgacg gccgcctgag accgaggggc agccctgttc ccgatgcggc
    35341 catacatgcg cccggcgtgg ccggtgcagg cgcagcgctg gttgctttcg ggagcttcca
    35401 cgacctcggg ctcgtggccg tcggcggctc gacgccgctt tccgacccga tcagccgcgg
    35461 acgatgatct tcacctggtc gcccgcctcg agctggcgac cgcgatcgaa gccgttgagc
    35521 gtgatgaact gctccctggg caaccgctcg acctccatgc gccgggccag gctctcgatg
    35581 gtgtcgccct cgcgcacctc gatgatctcc acccgcagcg gccgcagcgc ggccgcctcg
    35641 tctttcgtca ggcgatgcag gctgtccgcg gcggcgcgca gctcgcgcgc ctcgttccgg
    35701 ccgaagcgac cggtgatcag ggcgaaccgg tagaactcgc gcccgccgcc cgacagcacg
    35761 gcgaagcccg cctgggcgtt ctggccgccg acctgcacat ccgcatagcc gatggcaccc
    35821 tcgagcccgc catcgctggt gaaccgctcg acgccgtgca gctccagacc tttcagccat
    35881 tcgccctgca ggtaccgcgc cgggtcggtg gcctgcctgg cggcccccat gtcgaagagc
    35941 agcaggcggt tgtcggcccg gcccaccact gccgtgggct ggttgctcaa ccgatagggc
    36001 tccgggtagg cgaaggcgat gccgaggccc ggatgggcga agcggctgcc ctggacgaag
    36061 ccctgctcgg ggctctgccc gtagacgatg ccgtcgatcg cggcgaggaa cgccgcccgg
    36121 ttgcgctcgt cgcccgagct gccgcgctcg tccgcctggt cgcgcgcctc ctccacccga
    36181 tccggcgtgt agggatggct cgagaagaag tggtcgaagg cgcccggctc cttgacgctg
    36241 gccccgacga gcctggcctg cagctcggcg ctggtctgca gcgcctggag gaaatcggcc
    36301 gtcgccctgg tgtcgtagcc ggcccggtcg agatagcgga tgccgagcgt gtcggcctcg
    36361 tattcctggc tgcgcgagta ggtctgggtg agcccgtagc cggtctgctg gccgagctgc
    36421 gccccgagcc gggccccctc ctcgccgccc agcaccgcac cgcccagcac cccgagcagg
    36481 ccgaggccga aggtggcgag ctggcccgtc gtctgccgcg aggcactgtg ccgcgccgtg
    36541 acatggccga tctcgtgcgc caggaccccc gccagctccg cctcgtcgtc ggcataggcc
    36601 aggagccccc ggctgacata gacatagccg ccgggcaggg cgaaggcgtt gaccacctcg
    36661 ctgtcgagca cggtgaaggt gaagtcgatc t
//
LOCUS       DAOTFI010000009        35259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1336_length_35259_cov_6.713839, whole genome shotgun sequence.
ACCESSION   DAOTFI010000009 DAOTFI010000000
VERSION     DAOTFI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 35259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35259
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1336_length_35259_cov_6.713839"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="P5558_01570"
     CDS             complement(<1..562)
                     /locus_tag="P5558_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY23030.1"
                     /translation="MEADLIIRDATLPDGRGGCDIVIADGLVQAVGPSIPAAAREEID
                     AQGRLVSPPFVDCHFHMDATLSLGLPRLNQSGTLLEGIALWGELKPLLTHEAVIGRAL
                     AYCDLAVARGIGAIRSHVDVCDDRLLGVEALLEVKERVAPYLELQLVAFPQDGLYRGA
                     TARANLLRALDLGVEVGGPGLDPLIGD"
     gene            complement(574..1515)
                     /locus_tag="P5558_01575"
     CDS             complement(574..1515)
                     /locus_tag="P5558_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23031.1"
                     /translation="MDVLEILLQASFWAATLRIATPLIFGTLGELICERAGVLNLGIE
                     GIMTIGAMSGWLFVFLGVDLWTGILLAAAIGAGFGLLHGLLTVPLGLSQHVTGIGITL
                     LASSLAYFTYRVLLPDVATPPRIEPFQPMAPDWLLAVPFLGPVAAQQTALTWLALGLV
                     PLLSWVLYRTPLGLALRTCGENPVAAEAQGLDVYVIRIGAVMAGSALMAVGGAFLTLS
                     AFNAFFFEMVSGRGWICIALVVFASWIPWKALLGALLFGAFDAFQVRLQQVVGGAVPY
                     QLFLMLPYVMSILALLVMARRAAYPQALMRPYVKGQR"
     gene            complement(1516..2442)
                     /locus_tag="P5558_01580"
     CDS             complement(1516..2442)
                     /locus_tag="P5558_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY23032.1"
                     /translation="MSKGRVVSTGENLRAAGLVCLVTALFAVSDTVMKHLFQTVPVGQ
                     VMGLRGILICLLLLAVLVLRRRRFVWAHAASPVSLARAILEVAISLSFFMSLLLMPLA
                     DATVLLFAAPIIMTVMATLFLGEQVGVRRWSAVLVGFLGVLLVAGPTNRTLGVEAVFP
                     LLAATLVAIRDVITRYVPLEHDSTTVALTTAIAVSLAGCLSLPLGWTGLIAPWVWPGL
                     ADLVKIAVGAVLIATAYIAVVAAYRLGEMSFLAPFRYSSIPLVVLLSWTVFGDRPTPS
                     MLVGAAIITFSGIFIFTRERQLARRAQTAGPG"
     gene            complement(2439..3485)
                     /locus_tag="P5558_01585"
     CDS             complement(2439..3485)
                     /locus_tag="P5558_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23033.1"
                     /translation="MRLEPRASTAPWLNVAAPLGAALLALLICALPLALAGADVPEAY
                     RLMLAGSVGTRFALTETLTRAAPLILTGLAAAIAFRARLWNIGAEGQLYLGALAAVAV
                     GTGFVPVPGFLLLPLVVVAGALAGALWMLGPAWLRLRFGVDEVVTTLLSNFIVLLFVG
                     MMLEGPLQDPMSLGWPQSAPILDEASLPKLVERTRLHFGLVLALAAAILTWLGIQRTR
                     FGFELRAVGANPDAARFAGIAERRVMLKVALFSGAMAGLAGVGEVAGLKGYLTQDLSP
                     GYGYAGIVVATLAQLGPISVVASAIFIAGVFVGADSMSRAIGVSSYIANLIVATALLT
                     MLLAGLVTRYRIRR"
     gene            complement(3475..5031)
                     /locus_tag="P5558_01590"
     CDS             complement(3475..5031)
                     /locus_tag="P5558_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23034.1"
                     /translation="MTTSRDEPPLLRLRGITKRFGGLVANDGIDLDLARGEILALLGE
                     NGAGKTTLMSILFGHYVADAGTIEVTGADGAAHQLPPGSARAALAAGIGMVHQHFTLA
                     MNHSVLDNVILGTEPLWAWRQDLGAARARLAALIESTGLAVPMDARVGSLSIGEQQRV
                     ELLKALYRSAKVLILDEPTAVLTPQQSALLFTTLRRLAQGGLGIVFISHKLEEVMSLA
                     HRVAVLRHGRKVLEVAATGTDKARLAHAMVDRPVELPKALPQPAGAPALELLGVGVAP
                     AAGVEPLVDVDLTVHAGEIVGIAGVSGNGQRSLAGLVAGTLLPDRGSLRLAGAPWPTG
                     GPKALIAEGVGRIPEDRHRQGVVGDMTIWENLALEGYDAPDCRRFGLLDLARFRRRAQ
                     ALRERYDIRGGGSETPARLLSGGNMQKLILARVLEPGPRLILAAQPTRGLDIGALAEV
                     HRHLLDARARGAAILLISEDLDELMQLADRVAVLYRGKLGPARPRAGLELARLGLEMA
                     GQGPAAADAA"
     gene            complement(5028..6032)
                     /locus_tag="P5558_01595"
     CDS             complement(5028..6032)
                     /locus_tag="P5558_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRY23035.1"
                     /translation="MSRDLLARRRMGGALLGSTALCLAMLAGAPALAEPLEVAAIYTV
                     PVEQQWVSRIHKALNAAVERGDITYSFSENVSNTDYERVMREYAEQGVDLVFGEVFGV
                     ERAAREVAADYPDVAWVMGSSFGPEEPNFSVFDNYIQECSYLTGLVAGAKTESGLIGM
                     VGGYPIPEVNRLMHAFMAGALEVNPEIDFLVTFIGSWYDPPKAKEAAFAMIDAGADIL
                     YAERYGVADAAKERGVLAVGNVIDTQEEYPGTIVASALWHMEPTVDQALGKVQVGEYA
                     AENYGPLSHLAVGGCSIAPMDDALVAADVQAMVAEKEAAIRDGSLAVAVDDSEPTSTR
                     "
     gene            complement(6103..7074)
                     /locus_tag="P5558_01600"
     CDS             complement(6103..7074)
                     /locus_tag="P5558_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRY23036.1"
                     /translation="MAVTRLAALTLLLLAVSACQAADWTHRHGADHPFAGAIVDLRSQ
                     RVIDEAALLAALGNADIVLLGETHDNRDHHLLQARLVGAMGSSVNGVVFEMIDTDRQQ
                     AVIEHLQEHPRDTAGLGAAIGWEESGWPDWSMYEPIAAATLAIDAQIVAGSLPREAIQ
                     GLLADGTKGLDPALAERTGLTEPLEPMLEANMLATISEAHCGLTPAERLPAMLLVQRG
                     QDAMMADRLAAIRGRDKSVLIAGSGHVRRDWGVPLYLRRSEPGLRVLSVAFVEIDPEL
                     EALPIDLPYDFVWFTTRRQPYGFDPCKAFREQLQQIEARAAGLPTLG"
     gene            7223..7732
                     /locus_tag="P5558_01605"
     CDS             7223..7732
                     /locus_tag="P5558_01605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY23037.1"
                     /translation="MSNAAFGRPDLRRARPADLDRLVELERLFPGDRLSRRALRHHLR
                     NPKAHMIVSDRAGRVVGYALLLCPSRSSWWRLYSIVRAEGEAPPGTGHALVQAALEAA
                     RRAGARGIRLEVRADNQKAIKLYESSGFRLFGTRPGYYEDGATALRMALDFHGQEDVG
                     LADRRRPQG"
     gene            7683..9152
                     /locus_tag="P5558_01610"
     CDS             7683..9152
                     /locus_tag="P5558_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HRY23038.1"
                     /translation="MDKKTSAWLIVADRKDDFPAMPSGAAAIGTRDYLALGVRTGRAV
                     RKVLNLARDTSYQARGYYVSLLAEARGDKVVPSVATILALRRRSGYAFALPDLEEALN
                     RTARRLAEPPTEAFRLRLFLGRASDQRFARFGLLVFDWFRAPVLEITIQPGEWWRIKR
                     VAMLALGDLDADDRATLATAIARYVDRRWLEPKGRSEPRFTLAVLYDAKDPLPPSDLP
                     TLRRFARIAEGMGLGVDLIGKDDLAKLAAYDALWIRVTTNIDHYTYRFAQRAEQEGMP
                     VIDDPHSIMRCTNKVFLAELLTAHKVPTPPTRIIAGRRDIEPAAAALGYPIVLKIPDG
                     SFSRGVKKADDRAALDRLVADMLEESDLILAQAFMPTTFDWRVGVLNGEPLFVSKYHM
                     AKKHWQIVKHGSDGRTEAGRFNTMPITAAPPEVIDIGVRAARLIGRGLYGVDIKETPQ
                     GIVVIEVNDNPDLNHGIEDQAEKDAVWRQLASWFLERLR"
     gene            complement(9412..11013)
                     /locus_tag="P5558_01615"
     CDS             complement(9412..11013)
                     /locus_tag="P5558_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY23039.1"
                     /translation="MRPAGGGIVARLAAVALCLLSTPLPTNAQETTRTLLPAPVIPVP
                     ASIDRADVDVGVAALDGIVSEAMQATGVPGVAVAIVYQDDVIFEKGYGLREAGRPEPV
                     DPDTVFLLASLSKPITSTVVARLSDLHHFELDEPVGHHDPDFRLADPYVSEHATFIDL
                     LSHRSGLATGAGDFLEDLGFDRDTILDRLHMQPLDDFRSTYHYSNYGYTAGGVAAAKA
                     AGGVFEELAEEVLFQPLGMTRTSYRNADYLAHENRARIHVRNDAGEWEARFHRDPDAQ
                     APAGGVSSTVNDLAEFLRLQLAGGMRDGERLIDPAVLTVTHKPHSVSSNRPDSAARDG
                     FYGLGWGVSYDDIGRVVLSHSGAFDLGAATNLTLLPGEGLGIAVLSNGMPVGVVEAIA
                     TTFVDIATHGEQTVAWLALFGGAFDAMREGERADAAAAIPTGDPGPELPLEAYTGAYA
                     NSYFGLLEIRVEGGQLMATMGPRTAPVTFTLTRDEGSRFVFETIGEWATGPSVAFFSG
                     AEGGRPARVLFAAYDMQGLGTFERE"
     gene            complement(11228..11782)
                     /locus_tag="P5558_01620"
     CDS             complement(11228..11782)
                     /locus_tag="P5558_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY23040.1"
                     /translation="MSIHEVDPVVVADWLAAGGASLVDVREADEFRREHIKGSRSMPL
                     GAFDVPAFQDQVGREPSRKVVLLCSSGARARQAAQAITEAAGAEVWLLAGGLQAWKRA
                     GLEVERGTNAPLPLMRQVQIVAGGLALLGFILGVLVSPWWHALSGFVGAGLLMAGVTG
                     FCGMANLLQVMPWNRPGGPARAGS"
     gene            complement(11853..13208)
                     /locus_tag="P5558_01625"
     CDS             complement(11853..13208)
                     /locus_tag="P5558_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY23041.1"
                     /translation="MPDSGLATANPARLDATTAADRIARGRLAIDRLTDACLDVVAAR
                     EPELLAWAFIDPAYCRRQASTLIDGLAKGQTPGRLLGVPVGVKDIFATADMPTANGTA
                     LHAGRQPAADSAVVERLRAAGAIVLGKTVTTELAAFTPGPTRNPHDPTRTPGGSSSGS
                     AAAVAANMVPLAIGSQTNGSVIRPASYCGVYGFKPSFGLISRRGMTLQSPTVDHVGVF
                     ARRLADAALLVEVLAGYDSADPAMRPRATPPLVAAALAGRTRAPRLALIDDFAWETPG
                     PSTLAVFDEIARALPGVVDRTELPPGFAAAKDVHQSISWAELAHELADLHAAHPEGLS
                     DGLRAMIEWGQRLGAGAYLAALAERRRLQAAVLPLFDEFDAILTPAAAGEAPVGLGST
                     GSPGFCTLWSLLGTPAVCLPLRHGPSGMPLGVQLVGRLGEDAKLLATAAWLEQALAPG
                     S"
     gene            complement(13704..16418)
                     /locus_tag="P5558_01630"
     CDS             complement(13704..16418)
                     /locus_tag="P5558_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY23042.1"
                     /translation="MLRGLSLRWRIVLALFALQLALCAAILFFTLRQHLASQVELWQR
                     SDRYFVTLLSDLGRVALLTTDYTELQAFVAETTSSRKFTDMVLVDLGGRIVASKNLNR
                     IGSMFEPDTSKLDEGQRIVDISAGSHKLGTLALTLSDDALHASFREAYRRGAMVAGGG
                     IILIALSAYWFGHLLSHRLERLAATADRISLGDNEARATIEGQDEIARVGRAVNTMLD
                     RLTDNLSALRLAHDRLTLPTEAMNEGFAIWDANDRLVLSNTRFRRFFRNVPVAAISLP
                     FPELLDRHIRPLLALDDAAYATWRQARLACRRNGGICQFQFLDGRCIEISESRMEDGG
                     TVGIYSDITDAVARKSALNRSEARLRTIMGSVTEAIVTFDGQGLIDSANPAAERLFQT
                     TGEMLRGRRMAELLTCDDAEAGELPRLLTGTKGQSIEATGNRTDGSQFPLELTINRAS
                     LEGKCYYVAALRDLTRQKEAQAVILHQATHDPLTGLPNRTLFNERLGEILKHAQRCGE
                     LVAVAFLDLDRFKAINDSLGHAMGDALLVQLGERLQRSVRDEDTVARMGGDEFILILR
                     GLRKPHDATIPARKILASLQAPMHLEGHELRVTASLGISLYPIDAENRDTLLRHADMA
                     LYWAKSQGRNTFRLYDAALHSDTAERVRLESDLRRAVDDAELGLVYQPQVEVGSGRVV
                     GFEALMRWSHPERGQVPPDQFIGLAEDCGMIGQLGTFLLDRTTSDLKVWQRRNFVPDR
                     LAINISPLQIGRGDLVGQIRRFLDTSRLQPEQLELELTETALLEADRRSQRTIHALHR
                     LGVSLALDDFGTGFSSLSNLQRFPVQRVKIDRSFIRNMPSRSRDATLAKAIIGLAHGL
                     GMRVVAEGVESTQQLDMLNEFGCDEWQGFLKSRPIPANAVPDLCLTAA"
     gene            complement(16418..17266)
                     /locus_tag="P5558_01635"
     CDS             complement(16418..17266)
                     /locus_tag="P5558_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY23043.1"
                     /translation="MRSAPARRDALRLLAASLAMSATPAAAIPQRPAEPPLTFGVFPF
                     LPALEIGRRFGPVSSALAEMLGRPVALQTKTDFEAFRRFLLDGRYDLAFLHPFLYADA
                     ASAQDYRPLGRIREDLAAFIVARKEERCASFRDLCGRVLALPPKLSGVAQLAFHELEE
                     QRLLGPGDVQVEYHRTKSSCIHAVISGSAAACALPGFVLSQLKAFAPIELEPKFQTRA
                     VPGLLLAAHGRLGEPMLDQLRDLVIGLERVPAGQSLLAGFGWTGFVPAPPAQYDITHL
                     RGLVGG"
     gene            17461..18339
                     /locus_tag="P5558_01640"
     CDS             17461..18339
                     /locus_tag="P5558_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRY23044.1"
                     /translation="MSVSIRPRRSVLYMPGSNRRALVKGRELPADALILDLEDAVAPD
                     AKEAGREEIAGALAERESYGARELVVRVNGLDTMWGHGDLVAMAKAGADAILLPKVES
                     ADMVRRAVAVLDNAGAPADLMVWCMMETPRGILQVEAIADHHRVACLVMGTSDLTKDL
                     MARHTAMRLPMLPSIGLCLLAARAAGIAIIDGVYLDLDDQAGLVTACEQGRDLGFDGK
                     TLIHPKQIAAANDTFGPTDDAIQSAKRIIEAYAEALRAGKGVTVLDGKLVENLHVAEA
                     ERIVALAEAIADRAGA"
     gene            complement(18364..18966)
                     /locus_tag="P5558_01645"
     CDS             complement(18364..18966)
                     /locus_tag="P5558_01645"
                     /inference="COORDINATES: protein motif:HMM:NF040005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HRY23045.1"
                     /translation="MPSLAATMPMNRTFAIFREGDEIGRHHVRFTPADNGFRARTEIE
                     IEVKVAFFTAFQFRQTADDLWIDGQLRESRASTDDNGDVSETFIRATGNGLAVEGGEA
                     NRAIQVPLGTMTDLAFWNVAIVRQQALVDTQKVKLTDLAAKPLGIETVEGPHGPVTAD
                     RFTCAATTGRSGDIWFDSDGNWVKGIMSVRGETLAYRLIA"
     gene            complement(19105..19377)
                     /locus_tag="P5558_01650"
     CDS             complement(19105..19377)
                     /locus_tag="P5558_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRY23046.1"
                     /translation="MAARLRIYGRVQGVGYRAWLIRIAEARGIDGWARNRADGSVEAL
                     LHGDPEMVQAVVERCHRGPRLARVDRIEQAPDGSVPDRGFVQRPTA"
     gene            19710..21086
                     /locus_tag="P5558_01655"
     CDS             19710..21086
                     /locus_tag="P5558_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23047.1"
                     /translation="MTAEIARLLDRLHASGKVHGGVEPLAVLFVGGAVRLADPLPPGK
                     GVGADEVHASATAGASAPNGRQHDFECLGQTFALMLGGMERIEELPAAMRAVLARCGA
                     SDPATAFRSGNEIAEAIELALTAGEPPANAGRLEAAATSVQKARSVEPPASPVNARRR
                     RPGAGHRSLLLMLCGLLLLGGAAIFLLRPAPQVEAPAGREASGPNVPTPEAVEEKEAL
                     EELLAALPDQPLPEEPDRPTAETVLREILENADNRPCQRLEIERNGPDLRLVGRTASA
                     SDRASIMARLDTLEEAVDTAIDIDESNRFCNLYDILKRRTAIAWPRLARLYPRRTSHR
                     LSYGEELMIQVQTPAEPSHLIVDYFTADGMVVHLRAAAGGSTRLAPFTEIVVGDPLDG
                     SSLRIGPPSGNEVILVLASTAPLFSAARPPTEKADTYLAALDAALGQTAARPIASTLT
                     IETVPSDN"
     gene            21183..22295
                     /locus_tag="P5558_01660"
     CDS             21183..22295
                     /locus_tag="P5558_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY23048.1"
                     /translation="MTDEEEQDQRASLNLAFAIGGMNHFRVDVASGLIAGGPLVKRLL
                     GEPDAGEAFRVERLLTRLDATTGRQLAQILTDLPRHDLAVDVELVVVDHAAIDAIRMV
                     GRARTIPTPILAGIMVDETERRRSEELLRGRAADLAAANRQLEQFTHIVSHDLKSPLR
                     GVHHLASFLREDLGDAASPQVLEHLDKLDRQVTKMSRMLDDLQTYVHVDRRAGKIETV
                     DLEALGVELGELVGLPDGFTLDVDVEPVRVETDRTALSLVLRNLLDNSFKHHDRKSGM
                     LRIEARPHGRGRQRFRVIDDGPGIVRRYRKRVFEPFHCLGGAGPGSGMGLAFVKRAVE
                     RQGGRVWIEDGPGADAGRGVAFCFTWPLVGCGKPDG"
     gene            22288..22722
                     /locus_tag="P5558_01665"
     CDS             22288..22722
                     /locus_tag="P5558_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY23049.1"
                     /translation="MADGPGTHILLVDDDEIDVIALTRAFAEVGIDVPLIIARDGEEA
                     LGALLERGLDAVLMLLDLNMPRMGGFELLERRASDPRLGAAAVLVMTTSSRRQDIERA
                     HALGIVGYVVKATRPGFSRDFAELVRRYLALASLPRAGGRTR"
     gene            complement(22741..23667)
                     /locus_tag="P5558_01670"
                     /pseudo
     CDS             complement(22741..23667)
                     /locus_tag="P5558_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
     gene            24048..25388
                     /locus_tag="P5558_01675"
     CDS             24048..25388
                     /locus_tag="P5558_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRY23050.1"
                     /translation="MTPVDAAQLEHRYSSRTATLGIMGMGYVGLPLMLAATGAGFRVI
                     GFDIDLPKVEGLNAGLSPLAHIGHAPIAAARESGRFEATGDFGRIGETDAILICVPTP
                     LGRHKEPDLSYVVGTIEAMAPHLQPGQLIVLESTTWPGTTDEIVRPLVEAAGFHSGSD
                     IFIAYSPEREDPGNSEFRTGTIPKVVGGDGETALRLACTLYRQFVSHVVPVSTPATAE
                     AVKLTENIFRAVNIALVNELKLVLERMDIDIFEVIDAAKTKPFGYMPFYPGPGLGGHC
                     IPIDPFYLTWRAREFGIATKFIELAGEINTAMPQYVLGRAAEALDRQQGKGLSGATVL
                     LLGVAYKKNIDDMRESPALAIWELLRDRDATVTYHDPNVPVIPATREHASLTGSRSVP
                     LDRAVVGAVDLVVIVTDHGSVDYSLVAEAARLVVDTRNVMAPFAARMGSRLARA"
     gene            25463..26665
                     /locus_tag="P5558_01680"
     CDS             25463..26665
                     /locus_tag="P5558_01680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY23051.1"
                     /translation="MALIARSLARIKPSPTIAVTNLARELKAAGRDIIGLGAGEPDFD
                     TPEHIKEAAIEAIRAGDTKYTAVDGTPALKEAIIAKFARENSLVYGMQEISVGTGGKQ
                     VLYNAFMATLDPEDEVLIPAPYWVSYPDMVLLAGGKPVAIDCTAEAAFKLQPDQLEAA
                     ITPATKWLILNSPSNPTGAAYTEGELKALADVLLRHPQVHVMTDDMYEHLVYDDFRFT
                     TIAQVEPALQARTLTVNGVSKAYAMTGWRIGYAGGPAAIIKAMGMVQSQSTSNPSSIS
                     QAAAVAALNGPQDFLPERNAVFAQRRDLIVDLLNEAPGLSCPSPEGAFYVYPSCAGVI
                     GRKSPDGAVIDSSEAFARFLLEDGGVAVVHGSAFGLDPHFRISYATATEQLEEAGKRI
                     IATCQKLG"
     gene            complement(26677..27174)
                     /locus_tag="P5558_01685"
     CDS             complement(26677..27174)
                     /locus_tag="P5558_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRY23052.1"
                     /translation="MSGKRSFGRRGVLGVMALLPAAWVSGSAAQDAGGTPQERLAIEP
                     LAIRTKAGTVHFLVEMARSPAEQSKGLMFRVDVPDGTGMLFVHEPPRPVAMWMKNTPT
                     SLDMLFIDATGLIRKIDARTTPYSEAVLASGGPVAGVLEIRGGEAERLGIAVGDVVHH
                     PHFGR"
     gene            complement(27171..27929)
                     /locus_tag="P5558_01690"
     CDS             complement(27171..27929)
                     /locus_tag="P5558_01690"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRY23053.1"
                     /translation="MRLGFLAADTPDAGRALHDLTARYPSVEADSADVVVALGGDGFM
                     LEVLHRFIDRDVSIYGMNLGTVGFLLNAYTPDDLLGRIAGAMAVDLYPLRMCAERASG
                     GEVRGLAINEVSVFRQTRQAAALSIEIDSVKRLPELVCDGVMVATPAGSTAYNLSAHG
                     PIIPLGSNILALTPISAFRPRRWRGALLGSRTRFRIRVLDPIKRPVSAVADFTEVRDV
                     TSIEIWEDKTHGFRLLFDPEHNLEERILKEQFIP"
     gene            28088..29086
                     /gene="moaA"
                     /locus_tag="P5558_01695"
     CDS             28088..29086
                     /gene="moaA"
                     /locus_tag="P5558_01695"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRY23054.1"
                     /translation="MLVDGFGRQISYVRVSVTDRCDFRCVYCMAENMVFLPKRDLLSL
                     EELERLCDAFIQTGVRKIRLTGGEPLVRRGVMSLIQALGRRLGPVPGTLEELTLTTNG
                     SQLERHAEGLVAAGVRRINVSLDTLRPGRFEAITRWGRFEQVMQGIAAARSAGLAIKI
                     NMVALKDVNEDEFDQMIAWCGAEGYDLTLIETMPLGDIDGDRTDQYLPLSLVRARLQQ
                     RWRLTDLDCSTGGPARYVRVEETGGRLGFITPMSHNFCESCNRVRVTCTGTMYMCLGQ
                     EDAIDLRTPLRGAEDNGPVVAAIEAGIGRKPKGHDFVIDRRRKRPALARHMSVTGG"
     gene            complement(29109..31589)
                     /locus_tag="P5558_01700"
     CDS             complement(29109..31589)
                     /locus_tag="P5558_01700"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HRY23055.1"
                     /translation="MSLVSELSSQVLPAQPLLDALPIAAALLDSSGPSPASVRVVAAN
                     AAWLALWQDGELTGDAGPSQGETCFVAAADHADAMAHIAERRDFDREIALDCDGPAVV
                     RLHGRPADPAYLMTAELVSDTDTLARAWPGTALLPPPPGSFRYFAVLAPDGKWRIVAS
                     QPRFSRVLGLADTAEGWLHLAERAARPALRRRHLELLQGVPQSSTYRLCHAARSRITL
                     HDTAVPVRHPASGEVVGLQGCAWIVDPLPDPRPPGAIEPSFATLPATLGSTSAQSALV
                     EALGLLGVSSILLDPTGHVVGCSGDFGAALGQSTETIIGQSIRCLSLPDSVAVEQFVR
                     GGKDPAAAPSLTVPVGAPAHLTPRFVLQRLSICGGGHVLVTLAGEGSSGRSLRDTLYH
                     DPATALPNRFLFLDRLRQAVARATLAGSGLTVMVLGIDRLNLVERSFGRAAIDQLLTA
                     LVRRLEQSIGRLDTLARTDEMRFAILAFGHEGADGAAKLARVMLDELLEPVVVDGREI
                     AVGGMVGIAVFPNDGDQAETLLANAEAALDRLPAGRGQVFQFFSNALNAVSFDRLMLE
                     RQLVEAVERGEFLLHYQPQISFASSRIVGMEALIRWQHPELGMIPPGEFIPLAEETGA
                     IIAIGEWVLEAACRELQTWFDAGIAPLRLAINISGRQYNNRNLVPHVRQTLEATRFPA
                     RLLEFELTESVIMEDVIDATRRLGELDELGISLAVDDFGTGYSSLSYLKRFPIRSLKV
                     DRSFVRDIAHNEASAAIARAIIAFGSALGLKIIAEGVESPEQYEILRGCGCHELQGYL
                     FSRPIPAEEAHKLVLGSRQMTLAGRQIE"
     gene            complement(31774..33240)
                     /locus_tag="P5558_01705"
     CDS             complement(31774..33240)
                     /locus_tag="P5558_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRY23056.1"
                     /translation="MLCGTVLSTQAMAQVDRAAIAAGISGARLMENAGAGVVRAITGR
                     LSPGRVLLLCGPGNNGGDGYVIARRLAAAGWPVRVAALGDPARLKGDAALAAARWNGP
                     IGRLTEPAAGSFDILVDALFGAGLDRPLDPALGARLHELAAGRPQVVAVDVPSGVDGT
                     TGTVSPDTLPADLTVTFACLKPAHLLEPARSFMGEIRCVDIGIPEAAVAAHDTGLRVN
                     GPAEWGAMLPRWAAQEHKYSHGHVLVLGGGATSTGAASMAAIAAARVGAGLVTVAAPR
                     EALPIYASHAPTLLTRPMENVADLGRLLEDRRFTAMLVGPAFGLGRHSLAVVERVLDA
                     RLPCVLDADALTALGDSHLSIDERKLPASTVLTPHDGEFARLFRFTGDRLDRARRAAA
                     CAGCIVLLKGADTVIATPDGGARINAQAPAKLATAGTGDVLAGLVVGLMAQGMPAYDA
                     AAAAAWLHARAAERCATPMLATDLAGWIPQILGELSGD"
     gene            33377..33697
                     /locus_tag="P5558_01710"
     CDS             33377..33697
                     /locus_tag="P5558_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRY23057.1"
                     /translation="MSALFDFARLASFAEDDRTLEQELCGMFLVTADRYLDDMHRAMG
                     CAETWAAEAHRLKGAAANIGADAVAELALAAEHGQPDPGRLAQLQDALAATRQLLEKH
                     LGRG"
     gene            33996..34820
                     /locus_tag="P5558_01715"
     CDS             33996..34820
                     /locus_tag="P5558_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY23058.1"
                     /translation="MCSALVACGGIDPNIHDDADLYAYDIDDVRSMAPQGAPFTQGLR
                     SGYLSLADSETVAMGLANSRHFQRKAVGSAKGQAVQPDFVSLRTLSTGQIDELTAARA
                     RLMAGLDGGGRQKAPAAAARAQTSFDCWLAFEESNDAAGISACKKAFEDAMAEVERAL
                     ATDIGNVYLVFFAWDRADVTPVAATILDQVAQDYKDGEIPRVVLAGHTDTSGTADYNL
                     RLSERRARAIAAELVARGVPETALEVTWFGETELRVPTADGVREPQNRRVEILFAD"
BASE COUNT         5918 a        11711 c        12248 g         5382 t
ORIGIN      
        1 cgtcgccgat cagcggatcg aggccgggac ctcccacctc gaccccgaga tcgagggccc
       61 ggagcaggtt ggcccgggcg gtcgcgcccc gatagaggcc gtcctgggga aaggccacga
      121 gctgaagctc gagatagggg gccacgcgct ccttgacctc gagcagcgcc tcgacgccga
      181 gcaggcggtc gtcgcagacg tcgacatggc tgcggatggc gccgatgccg cgcgccaccg
      241 ccaggtcgca ataggccagc gcccgcccga tcaccgcctc gtgggtcaaa agcggcttga
      301 gctcgcccca gagtgcgatg ccctcgagca gcgtgccgga ctggttgagg cgcggcaggc
      361 cgagcgacag cgtggcgtcc atgtggaagt ggcagtcgac gaagggcggg ctgaccagcc
      421 ggccctgggc gtcgatctcc tcccgggcgg ctgcagggat cgagggcccc accgcctgga
      481 cgaggccatc ggcaatgacg atgtcgcagc cgccgcggcc atccggcagc gtcgcgtcgc
      541 gaatgatgag gtccgcctcc aaggcatcgt tccttaccgt tggcccttga cgtaggggcg
      601 catgagagcc tgcggatagg cggcccgacg ggccatgacc agcagcgcca gtatgctcat
      661 cacatagggc agcatcaaga agagctggta gggaaccgca ccgcccacca cctgctgcaa
      721 cctcacctgg aaggcgtcga aggcgccgaa aagcagcgca ccgagcaggg ccttccaggg
      781 tatccaggaa gcgaagacca cgagcgctat gcagatccag ccgcggccgc tgaccatctc
      841 gaagaagaag gcgttgaagg cggaaagggt gagaaaggcg ccgccgaccg ccatgagcgc
      901 gctgccggcc atgacggcgc cgatgcggat gacatagaca tcgaggccct gcgcctcggc
      961 agccaccggg ttctcgccgc aggtgcggag cgccaggccg agcggtgtcc ggtagagcac
     1021 ccaggagagc agcggcacga gccccagggc gagccaggtg agggcggtct gctgggccgc
     1081 caccggcccc aggaacggca cggccaggag ccagtccggt gccatgggct ggaacggctc
     1141 gatgcgcggc ggcgtcgcga cgtctggcag cagcacgcga taggtgaaat aggcgaggct
     1201 ggaggccagg agcgtgatgc cgatgccggt cacgtgctgc gacaggccga ggggaaccgt
     1261 caagaggccg tgcaggagac cgaagccggc accgatcgcc gccgccagca ggatgccggt
     1321 ccagagatcg acgccgagga agacgaagag ccagcccgac atggcgccga tggtcatgat
     1381 tccttcgatg ccgagattga gcacgcccgc ccgctcgcag atcagctcgc cgagcgtgcc
     1441 gaagatcagc ggcgtggcga tgcgcagggt ggccgcccag aagctcgcct ggaggaggat
     1501 ctcgaggacg tccattcagc ccggtcccgc tgtctgtgcc cgccgcgcca gctgccgctc
     1561 gcgagtgaag atgaagatgc cggaaaacgt gatgatggcg gccccgacca gcatcgacgg
     1621 ggtcggccgg tcgccgaaga cggtccatga aaggagcacc acgaggggga tcgaggaata
     1681 gcggaagggg gcgaggaacg acatctcgcc gagccggtag gcggcgacga cggcgatgta
     1741 ggcggtggcg atcaggacgg ccccgaccgc gatcttgacg agatcggcga ggcccggcca
     1801 gacccagggg gcgatgagcc cggtccaccc gagcggcagg ctgaggcagc cggccagcga
     1861 cacggcgatc gccgtggtga gggccaccgt ggtcgaatcg tgctcgagcg gcacgtagcg
     1921 ggtgatgacg tcgcggatcg cgacgagggt cgcggcgagc agcgggaaga cagcctcgac
     1981 gcccagcgtg cggttggtcg ggccagcgac caggagcacg ccgagaaacc cgacgagcac
     2041 cgcggaccag cggcggacac ccacctgctc gccgaggaag agcgtcgcca tgaccgtcat
     2101 gatgatcggc gccgcgaaca gcagcaccgt ggcatcggcg agcggcatca agagcaggct
     2161 catgaagaag gagagggaga tggcgacctc gaggatcgcc cgcgcgagcg agacggggct
     2221 cgccgcatgc gcccagacga agcgccggcg acggagcacg agaacggcga gcaggaggag
     2281 gcagatcaaa ataccgcgca gccccatgac ctggcccacg ggcacggtct ggaagaggtg
     2341 cttcatcacg gtgtcgctga cggcgaagag ggcggtgacg aggcagacga gccccgcggc
     2401 cctgaggttc tcgccggtcg agacgacgcg gcccttgctc atcggcggat ccggtagcgg
     2461 gtgacgagcc cggcgagcag catggtcaag agggccgtgg cgacgatcag gttggcgatg
     2521 tagctcgaaa caccgatggc ccggctcatg ctgtccgcac cgacgaagac gccggcgatg
     2581 aagatggcgg aagcgaccac cgaaatgggc ccgagctgcg ccagcgtcgc cacgacgatg
     2641 ccggcatagc catagccggg cgacaggtcc tgggtcaggt agcccttgag ccccgccacc
     2701 tcgccgacgc cggcgaggcc ggccatggcc cccgagaaga gcgccacctt gagcatgacc
     2761 cggcgctcgg cgatgccggc gaagcgggcg gcatcgggat tggcgccgac cgcccgcagc
     2821 tcgaagccga agcgggtccg ctggatgccg agccaggtca ggatggccgc ggccagcgcc
     2881 agcacgaggc cgaaatggag gcgggtccgc tccaccagct tcggcaggct cgcctcgtcc
     2941 aggatgggcg cgctctgcgg ccagccgagg ctcatcgggt cctgcagggg cccttcgagc
     3001 atcatgccga cgaagagcag cacgatgaag ttggagagca gcgtcgtcac cacctcatcg
     3061 acgccgaagc gcaggcggag ccaggccggc ccgagcatcc agagcgctcc tgccagggca
     3121 cccgccacca cgaccagcgg cagcagcagg aaacccggga ccgggacgaa gccggtgccg
     3181 accgcgaccg cggcgagggc gccgagatag agctggccct cggcgccgat gttccacaag
     3241 cgcgcccgga aggcgatggc ggcggcgagg cccgtcagaa tcaggggggc ggccctggtc
     3301 aaggtctcgg tcagcgcgaa gcgggttccg acgctgcccg cgagcatcag ccggtaggcc
     3361 tccggcacgt cggcgccggc gagggcgagg gggagggcgc agatgaggag cgccagcagc
     3421 gccgcaccga gcggagccgc gacgttcagc cagggagcgg tgctggcgcg cggttcaagc
     3481 cgcatcggct gcagccgggc cctggcccgc catctcgagc cccagccgcg ccagctccag
     3541 cccggcgcgc ggccgggccg ggccgagctt cccgcgatag agcacggcca cgcgatccgc
     3601 gagctgcatg agctcgtcga ggtcctccga gatcagcagg atggcagcac ccctggccct
     3661 ggcgtccagc aggtgccgat ggacctcggc gagcgcgccg atatccaggc cgcgcgtcgg
     3721 ctgggccgcg aggatcaggc gcggcccggg ctcgagcacg cgggcgagga tcagcttctg
     3781 catgttgccg ccggagagca gccgggcggg cgtctcgctg ccgccgccgc ggatgtcgta
     3841 gcgctcacgg agcgcctggg cgcgccttct gaagcgggcg aggtcgagca ggccgaagcg
     3901 ccggcagtcg ggcgcgtcgt agccctcgag cgccaggttc tcccagatcg tcatgtcgcc
     3961 gaccacgccc tggcggtgcc ggtcctcggg gatgcgcccg accccctcgg cgatcagcgc
     4021 cttcggcccc cctgtcggcc agggtgcccc ggcgagccgc aggctgcccc gatccggcag
     4081 cagcgtcccg gcgacgaggc cggcgaggct gcgctggcca ttgcccgaga cgccggcgat
     4141 gccgacgatc tcgcccgcat ggacggtgag gtcgacatcg accagaggct cgacccccgc
     4201 cgccggcgcg acgccgacgc ccaggagctc cagcgccggc gcgcccgccg gctgcggcag
     4261 cgccttgggc agctcgaccg gccgatccac catggcatgc gcgagccgcg ccttgtcggt
     4321 gcccgtcgcc gccacctcga ggaccttcct gccatggcgc agcacggcca cccggtgggc
     4381 caggctcatc acctcctcga gcttgtggct gatgaagacg atgccgagcc cgccctgggc
     4441 gaggcggcgg agtgtggtga agagaagcgc gctctgctgc ggcgtcagca ccgcggtcgg
     4501 ctcgtccagg atcagcacct tcgcactgcg atagagtgcc ttgagcagct cgacccgctg
     4561 ctgctcgccg atggagaggc tgccgacccg ggcgtccatg ggcacggcga gcccggtcga
     4621 ctcgatcagt gctgccagcc tcgcccgggc cgccccgagg tcctgccgcc aggcccacaa
     4681 gggctccgtg ccgaggatca cgttgtcgag caccgagtgg ttcatggcca gcgtgaagtg
     4741 ctggtgcacc atgccgatgc ccgccgccag cgccgcccgg gccgagccgg gcgggagctg
     4801 gtgcgcggca ccatccgccc cggtcacctc gatcgtcccc gcatcggcga catagtggcc
     4861 gaagaggatg ctcatcagcg tcgtcttgcc ggccccgttc tcgccgagca gcgccaggat
     4921 ctcgccgcgc gcgagatcga ggtcgatgcc gtcattggcg accagcccgc cgaaacgctt
     4981 ggtgatgccg cgcaggcgca gcagcggcgg ctcgtcccga gatgtcgtca ccgggtcgag
     5041 gtcggctccg aatcgtcgac cgccacggcg aggctgccgt cgcggatggc ggcctccttc
     5101 tcggcgacca tcgcctggac atcggccgcg acgagcgcgt cgtccatcgg tgcgatgctg
     5161 cagccgccga ccgcgaggtg gctaaggggc ccgtagttct ccgccgcgta ctcgccgacc
     5221 tggaccttgc cgagcgcctg gtcgaccgtc ggctccatgt gccagagcgc gctggcgaca
     5281 atggtgccgg ggtactcctc ctgggtgtcg atgacattgc cgacggcgag cacgccgcgc
     5341 tccttggcgg cgtcggcgac gccgtagcgc tcggcgtaga gaatgtccgc cccggcatcg
     5401 atcatggcga aggcggcctc cttggccttg ggcgggtcgt accaggagcc gatgaacgtc
     5461 acgaggaaat cgatctccgg gttgacctcg agcgcacccg ccatgaaggc gtgcatcagc
     5521 cggttcacct cggggatcgg gtagccgccg accatcccga tcaggccgct ctcggtcttg
     5581 gccccggcga cgaggccggt gaggtacgag cattcctgga tgtagttgtc gaagaccgag
     5641 aaattcggct cctcggggcc gaagctcgag cccatgaccc aggcgacgtc cggatagtcc
     5701 gccgccacct cgcgcgccgc ccgctcgacg ccgaagacct cgccgaagac gaggtccacg
     5761 ccctgctcgg catattcgcg catcacgcgc tcgtagtcgg tgttcgagac gttctcggag
     5821 aagctgtagg tgatgtcgcc ccgctccacg gcggcgttca gcgccttgtg gatacggctc
     5881 acccactgct gctcgaccgg caccgtgtag atggcggcga cctccagcgg ctccgcaaga
     5941 gcgggagcgc cggccaacat cgccaggcag agtgctgtgg aaccgagcag tgcgccaccc
     6001 atccgacgcc gtgccaacaa gtctcgggac atgccagttt ctccagggcc aatcgttggc
     6061 cggagtgtgg cgctgccgat caatgttgca aagggctttt cctcagccga gggtcggcaa
     6121 ccccgccgcg cgcgcctcga tctgctggag ctgctcgcgg aacgccttgc agggatcgaa
     6181 cccgtaaggc tggcggcggg tggtgaacca gacgaaatca tagggcaggt cgatcggcag
     6241 cgcctccagc tccgggtcga tctcgacgaa cgcgacactc agcacgcgca gacccggctc
     6301 ggagcgccgc aggtacaggg gcacgcccca gtcgcgccgc acatggcccg agccggcgat
     6361 caggacactc ttgtcgcgtc cccggatggc ggccagccga tcggccatca tcgcgtcctg
     6421 gccccgctgc accagcagca tggcgggcag gcgttccgcc ggcgtcagcc cgcaatgcgc
     6481 ctcgctgatc gtcgccagca tgttcgcctc gagcatcggc tcgagcggct cggtgaggcc
     6541 ggtgcgctcg gccagggccg ggtcgagacc cttggtgccg tcggccagga ggccctggat
     6601 cgcctcgcgc ggcaggctgc cggcgacgat ctgggcatcg atggccaggg tggccgccgc
     6661 gatcggctcg tacatcgacc agtccggcca gccgctctcc tcccagccga tggccgcgcc
     6721 gagcccggcc gtgtccctcg gatgctcctg cagatgctcg atcaccgcct gctggcgatc
     6781 ggtgtcgatc atctcgaaga cgaccccatt gacggacgag cccatggccc ctaccagccg
     6841 cgcctgcaac agatggtggt cgcgattgtc gtgcgtttcg ccgagcagca cgatgtcggc
     6901 gttgccgagc gccgccagca gcgccgcctc gtcgatcacg cgctgcgagc gaaggtcgac
     6961 gatcgcaccg gcgaacggat ggtccgcgcc atggcgatgc gtccagtcgg ccgcctgaca
     7021 ggcggagacg gccagcagca gcagggtgag tgcggccaga cgggtgactg ccaagcttca
     7081 agcctccgat ctttgccctt caggcggcgg acgcccagca cggcaacgcg ccaggaaggg
     7141 ggagcgtcct cggacaatgc tggagatagc ggtgtgagtg cccggcgcaa gagctcgtcg
     7201 ctcccgtgat gcgacacgga gtatgagcaa cgccgcattc gggcggccgg atctgcgccg
     7261 ggcgcgcccg gccgacctcg accggctggt cgagctcgag cggctgtttc ccggcgaccg
     7321 gctgtcgcgg cgggcattgc gccatcatct gcggaatccg aaagcccaca tgatcgtcag
     7381 cgatcgggcc ggccgggtgg tcggctacgc gctgctgttg tgcccgagcc gcagttcgtg
     7441 gtggcggctc tattccatcg tgcgcgccga gggggaggcg ccgcccggca ccggccacgc
     7501 cctggtccag gcggcgctgg aggcggcgcg acgcgccggt gcccgaggga ttcggctcga
     7561 ggtccgggcc gacaaccaga aggcgatcaa gctctacgag agcagtggat ttcgcttgtt
     7621 cggaacgcgc ccgggctatt acgaggacgg cgcaaccgcc ttacgcatgg cattggattt
     7681 ccatggacaa gaagacgtcg gcctggctga tcgtcgccga ccgcaaggat gactttccgg
     7741 ccatgccgag cggtgccgcg gcaatcggca cgcgcgacta tctggcgctg ggtgtcagga
     7801 cggggcgggc cgtgcgcaag gtgctcaacc tcgcccggga cacgagctac caggcccgcg
     7861 gctactacgt ctcgctgctc gccgaggcgc gcggcgacaa ggtggtgccg tcggtggcga
     7921 ccatcctggc gctgcgccgg cgctccggct acgccttcgc cctgccggac ctcgaggagg
     7981 cgctgaaccg cacggcgcgc cgcctggccg agccgccgac cgaggccttc cgcctccggc
     8041 tcttcctcgg ccgcgccagc gaccagcgct tcgcccgctt cggcctgctc gtcttcgact
     8101 ggttccgggc acccgtcctc gaaatcacca tccagccggg cgagtggtgg cggatcaagc
     8161 gggtggcgat gctggccctc ggcgacctcg acgccgacga ccgggccacc ctggccacgg
     8221 ccatcgcccg ctacgtggac cggcgctggc tcgagcccaa gggccgcagc gagccgcgct
     8281 tcaccctcgc cgtgctctac gacgcgaagg acccgctgcc gccatccgac ctgccgacac
     8341 ttcgccgctt cgcccggatc gccgagggca tggggctcgg cgtcgacctg atcggcaagg
     8401 acgatctcgc caagctcgcc gcctacgacg cgctgtggat tcgcgtgacc accaatatcg
     8461 accactacac ctaccgcttt gcgcagcgcg ccgagcagga aggcatgccg gtgatcgatg
     8521 acccgcactc catcatgcgc tgcaccaaca aggtcttcct cgcggagctc ctgacggcgc
     8581 acaaggtgcc gacgccaccg acccggatca tcgccggccg gcgcgacatc gagccggcgg
     8641 cagccgcgct cggctacccg atcgtcctga agatccccga cggctcgttc tcgcgcggcg
     8701 tcaagaaggc cgacgaccgc gccgccctcg accgcctcgt cgccgacatg ctggaggaat
     8761 ccgacctcat cctcgcccag gccttcatgc cgaccacctt cgactggcgg gtcggcgtgc
     8821 tcaacggcga gccgctcttc gtgagcaagt accacatggc gaagaagcac tggcagatcg
     8881 tcaagcacgg cagcgacggg cgcaccgagg cgggccgctt caacaccatg ccgatcacgg
     8941 cggcaccacc cgaggtcatc gacatcggtg tccgcgcggc ccgcctcatc ggccgcggcc
     9001 tctacggcgt cgacatcaag gagacgccac agggtatcgt ggtgatcgag gtgaacgaca
     9061 atcccgacct caatcacggt atcgaggacc aggcggagaa ggatgcggtc tggcgccagc
     9121 tcgcgagctg gttcctcgag cgcctgcgct gatccgccga gcaggaagaa aagtcggggc
     9181 aggcgagaaa aaaggccgat cctgtaacca tcgtcacggt tctgaccgcc caccccggct
     9241 agtgagggtg cgttctctcc tctcatccta tcgccttcgg ccgcccctcg gggcggccct
     9301 ttcttttggc gacggcatgc agaccgtgcc cgcctcctcc tgcagggagt ggctgcgtct
     9361 cttccggaga tcggatcgag gtcctgtcag gccccgggcc ccgcgccggc ctcactcgcg
     9421 ctcgaacgtg ccgagcccct gcatgtcata ggcagcgaag agcacccggg caggcctgcc
     9481 gccctcggcg cccgagaaga acgcgacgga ggggccagtg gcccattcgc cgatcgtctc
     9541 gaacacgaac ctgctgccct cgtcgcgggt gagggtgaag gtgacgggcg ctgttcgcgg
     9601 ccccatggtc gccatgagct gcccgccctc gacgcggatc tccaaaagcc cgaagtagct
     9661 gttggcgtaa gcgcctgtgt aggcctcgag agggagttcg gggccggggt cgccggtcgg
     9721 gatcgcggcg gcggcgtcgg cgcgctcgcc ctcgcgcatc gcatcgaagg cgccgccgaa
     9781 gagcgccagc cacgccaccg tctgctcgcc atgggtggcg atgtcgacga aggtcgtggc
     9841 aatcgcctcc accaccccga caggcatccc gttggagagg acggcgatgc ccagtccctc
     9901 gcccggcagc aaggtcaggt tggttgccgc gccgagatcg aacgcgccgg aatggctgag
     9961 cacgacgcgg ccgatgtcgt cgtaggaaac gccccagccg agcccgtaga agccgtcccg
    10021 cgcggcgctg tcggggcggt tgctgctcac cgaatggggc ttgtgggtga ctgtcaggac
    10081 cgccgggtcg atcagcctct cgccatcacg catcccgccg gcgagctgga ggcgcaggaa
    10141 ctctgcgagg tcattgacgg tgctggagac gcctccggcg ggggcctgcg cgtccgggtc
    10201 gcgatggaag cgcgcctccc attcgccggc atcgttgcgg acgtggatgc gcgcccggtt
    10261 ctcgtgcgcg agatagtcgg cgttgcggta gctcgtgcgc gtcatgccca aaggctggaa
    10321 caggacctcc tcggcaagct cctcgaacac cccgccggcc gccttggccg ccgccacgcc
    10381 gccggcggtg tagccgtagt tgctgtagtg ataggtggag cggaaatcat cgagcggctg
    10441 catgtgcaac ctgtcgagga tcgtgtcacg gtcgaagccg agatcctcca gaaagtctcc
    10501 ggcacccgtc gccagaccgg agcgatggga cagcaggtcg atgaaggtgg cgtgctcgct
    10561 gacataggga tcggcgagcc ggaagtcggg atcgtggtgc cccaccggtt cgtccagctc
    10621 gaagtggtgc aggtccgaca gtcgcgccac gacggtggag gtgatcggct tcgacagcga
    10681 ggcgagcagg aagacggtat ccgggtcgac cggctcgggc cggcccgctt cgcgcaagcc
    10741 gtagcccttc tcgaagatca cgtcgtcctg atagacgatc gcgacagcaa cgcccggaac
    10801 cccggtggcc tgcatggcct cgctcacgat cccgtcgagt gccgcgacgc ccacatccac
    10861 gtccgcccgg tcgatggaag ctggcacggg gatcacgggt gccggcagca gcgtcctcgt
    10921 cgtttcctgg gcattggtcg gcaaaggtgt ggaaagcaga caaagtgcca ccgcggcaag
    10981 cctggcgaca atgccgccac cggcaggccg caagcatgct gtctcgtccg acagatgact
    11041 tgctgcacgc actatggttg cgctccccgg tttttttcag cctaagcgcc ggcgagaccg
    11101 ctggcaacga ggcgtcgctc aggtgtgcaa cgatgccgcc gcactctaca gatcgagtga
    11161 atatctcctt gtcgtttctc gtcctcgcgg ccgcccctcc gggcggccgc ttctcacgtc
    11221 ggcgaggtca ggagcccgcc cgggccgggc cacccggacg gttccagggc atgacctgga
    11281 gcaggttcgc catgccgcag aagccggtga cgccggccat caggaggccg gcgccgacga
    11341 agccgctgag cgcgtgccac cagggcgaga cgaggacgcc caggatgaag ccgagcagcg
    11401 ccagcccccc ggcgacaatc tgcacctggc gcatcagggg caggggagcg ttcgtccccc
    11461 gttcgacctc gaggccggcg cgtttccagg cctggagccc gccggcgagg agccagacct
    11521 cggcccctgc cgcctcggtg atggcctgcg cggcctgccg ggcgcgtgcg ccgctggaac
    11581 agagcagcac caccttccgg ctcggctcac gaccgacctg atcctggaaa gccggcacgt
    11641 cgaaggcgcc gagcggcatc gagcgcgaac ccttgatgtg ctcgcgccga aactcgtccg
    11701 cctcgcgcac atcgacaaga ctcgcgccgc cagcggcgag ccagtcggcg accacgaccg
    11761 gatcgacttc atgaatgctc atgcccgtcc tcacatgcaa aatcggataa gtgtaatata
    11821 ggcgtggcgg ccgggcgtgc aagcggtccc gatcagctcc cgggtgccag cgcctgctcg
    11881 agccaggcgg cggtggcgag cagcttcgcg tcctcgccga gccggccgac cagctgcacg
    11941 ccgagcggca tgccgctcgg cccatggcgc agcggcaggc agacggccgg cgtgccgagc
    12001 agcgaccaga gcgtgcagaa gcccgggctg ccggtggagc cgagcccgac cggcgcctcg
    12061 ccggcggcgg ccggggtgag gatggcgtcg aactcatcga agagcggcag gacggcagcc
    12121 tgcaggcgcc gtcgctccgc cagggcggcg agataggcgc cagcgccgag gcgctggccc
    12181 cactcgatca tggccctgag cccgtcgctc agcccttccg gatgcgcagc gtggagatct
    12241 gccagctcgt gcgccagctc ggcccagctg atgctctggt gcacgtcctt cgccgccgcg
    12301 aagcccggcg gcagctccgt ccggtcgacg accccgggaa gcgctcgcgc gatctcatcg
    12361 aagacggcca gggtcgaggg cccgggcgtc tcccaggcga agtcgtcgat cagggcgagg
    12421 cgtggtgccc tggtccgtcc ggcgagagcc gcggcgacca gaggcggcgt cgcccgcggg
    12481 cgcatcgccg ggtcggcgct gtcatagccg gcgagcacct cgaccagcag cgccgcgtcg
    12541 gcgaggcgcc gggcgaagac gccgacatgg tcgaccgtgg gcgactgcag ggtcatgccc
    12601 cgtcggctga tcaggccgaa cgagggcttg aagccataga cgccgcaata gctcgccggc
    12661 cggatgaccg agccgttggt ttgcgagccg atggcgagcg gcaccatgtt ggccgccaca
    12721 gcggcggcgg agcccgagga cgacccgccg ggcgtgcggg tcggatcgtg cgggttgcgc
    12781 gtcggcccgg gcgtgaaggc ggcgagctcg gtggtcacgg tcttgccgag cacgatggcg
    12841 ccggccgccc gcagccgctc gaccacggcc gaatcggctg ccggctggcg tccggcatgc
    12901 agcgccgtgc cgttggccgt cggcatgtcc gccgtggcga agatgtcctt cacgccgacc
    12961 ggcacgccaa gaagccgccc cggcgtctgc cccttcgcca gcccgtcgat caacgtgctg
    13021 gcctggcgcc ggcaatacgc cggatcgatg aaggcccagg ccagaagctc gggctcgcgc
    13081 gccgccacga cgtcgaggca ggcatcggtc agccggtcga tcgcgagacg accccgggcg
    13141 atccggtccg ctgccgtggt cgcatccagt cgggcgggat ttgccgtggc gaggccgctg
    13201 tcgggcatgg gcggcgtggt tccctgaatg gtcgtgggcc aggacaatct ggcatcacca
    13261 tcggccggga aacgatgcct gtcgatagcg gccccgcccc ggatgtcgcg gaaaatgcaa
    13321 catcatcctc gggttgtcgc gtctggcgcg acgttgttat ggcaaatgtc gcggcagaag
    13381 cgacatttgc cgccgggatg tcgcgccaga cgcgacattt ttgccgggtt ggcgcgtcgg
    13441 aagcgacgtt ttcagacgag cgaacccagg aaaacgacga atgaacccac cgattcgacg
    13501 atcgaacccg agtattcgac gaatgaaccc gagctcgcac caacgaaccc gagacccatt
    13561 aagatattga tccagaacgt tttttgcctt cttcctgacg tcccgacaaa gggcgtgaga
    13621 accagaatgc caaaaatggc gaacgaaccg aagcgggctt gcttttccgg tgcatcaatg
    13681 ataggaataa gatcagaaga cgctcaggct gcggtgaggc agagatcggg cacggcgttg
    13741 gccgggatcg gccggctctt gaggaagccc tgccattcgt cgcagccgaa ctcgttcagc
    13801 atgtcgagct gctgggtgct ctcgacccct tccgccacga cccgcatgcc gagcccgtgg
    13861 gcgagcccga tgatcgcctt ggcgagcgtc gcatcgcgtg accggctcgg catgttgcgg
    13921 atgaacgagc ggtcgatctt cacccgttgg accggaaagc gctggaggtt gctgagcgac
    13981 gagaagcccg tgccgaagtc gtcgagggcg aggctgacac ccaggcgatg cagcgcatgg
    14041 atcgtccgct ggctgcgccg atcggcctcg agcagcgccg tctcggtcag ctccagctcg
    14101 agctgctcgg gctgcagccg ggaggtgtcc aggaagcggc ggatctgccc gacgaggtcg
    14161 ccccggccga tctgcagggg cgagatgttg atggcgagcc ggtccggcac gaagttgcga
    14221 cgctgccaga ccttcaggtc ggaggtcgtg cggtcgagca ggaaggtgcc gagctgcccg
    14281 atcatgccgc aatcctcggc gaggccgatg aactggtcgg gcggcacctg gccgcgctcg
    14341 gggtgcgacc agcgcatcag cgcctcgaag ccgaccacgc ggccggagcc gacctcgacc
    14401 tgtggctggt agacgagccc gagctccgca tcgtccacgg cccgcctgag gtcgctctcc
    14461 aggcgcaccc gctcggccgt gtcgctgtgc agcgcggcgt cgtacaggcg gaacgtgttg
    14521 cgcccctgtg atttcgccca gtagagcgcc atgtcggcat ggcgcagcag cgtgtcgcgg
    14581 ttctcggcgt cgatcggata gaggctgatg ccgaggctgg cggtgacccg aagctcgtgg
    14641 ccctcgaggt gcatcggtgc ctgcaggctg gccaggatct tgcgggcggg gatggtcgca
    14701 tcgtgcggct tgcgcaggcc gcgcaggatc aagatgaact cgtcgccccc catccgggcg
    14761 acggtatcct cgtcacggac ggatcgctgc aggcgctcgc cgagctgcac cagcagggca
    14821 tcgcccatgg cgtggccgag gctgtcgttg atcgccttga agcggtcgag gtcgaggaag
    14881 gcgacagcga ccagctcgcc gcaccgctgc gcgtgcttga ggatctcgcc gagccgctca
    14941 ttgaagaggg tgcggttcgg caggccggtc aggggatcgt gggtcgcctg atggaggatg
    15001 accgcctggg cctccttctg ccgcgtcagg tcgcgcaggg cggcgacgta gtagcacttg
    15061 ccttcgaggg atgcccggtt gatcgtcagc tcgaggggga attggctgcc gtccgtgcga
    15121 ttgccggtcg cctcgatgga ctggcctttg gtgccggtga gcagccgcgg caactcgccg
    15181 gcctcggcgt cgtcgcaggt cagaagctcg gccatccggc ggccgcgcag catctccccg
    15241 gtcgtctgga acagccgctc ggccgccggg ttggcgctgt cgatcaggcc ctgcccgtcg
    15301 aacgtgacga tggcctcggt gaccgagccc atgatcgtgc gaagtcgcgc ctcgctgcgg
    15361 ttgagcgcgg acttgcgggc gaccgcgtcg gtgatgtcgg aatagatgcc gaccgtcccg
    15421 ccatcctcca tgcgcgattc gctgatctcg atgcagcggc cgtcgagaaa ctggaactgg
    15481 cagatccccc cgttgcgccg gcaggcgagg cgggcctgcc gccaggtggc ataggccgcg
    15541 tcgtcgagtg cgagcagcgg tcggatatgt cgatcgagaa gctcggggaa cgggagggag
    15601 atggccgcaa cggggacgtt ccggaagaat cggcgaaagc gggtgttgga caggaccagg
    15661 cgatcgttcg cgtcccagat cgcaaagccc tcgttcatcg cctcggtcgg cagggtcagc
    15721 cgatcgtggg cgaggcgaag cgcgctcagg ttgtcggtca ggcggtccag catggtgttg
    15781 accgcccggc cgacccgcgc gatctcgtcc tggccctcga tcgtcgcgcg cgcctcgttg
    15841 tcgcccaggc tgatgcggtc ggccgtcgcg gccaggcgct cgagccgatg gctcaggaga
    15901 tggccgaacc agtacgccga aagggcgatg aggatgatgc ccccgcccgc gaccatggca
    15961 ccgcggcgat aggcctcgcg aaagctggca tgcagcgcgt cgtcgctcag ggtcagggcc
    16021 agcgtgccga gcttgtggct gccggcgctg atatcgacga tccgttggcc ttcgtcgagc
    16081 ttcgaggtgt ccggctcgaa catgctgccg atccggttca ggttcttgga ggcgacgatc
    16141 cgcccgccga gatcgaccag caccatgtcg gtgaacttgc gcgagctcgt ggtctcggcg
    16201 acgaaggcct gcagctccgt gtagtcggtg gtcaggaggg cgacgcgccc gagatcgctc
    16261 aagagggtga cgaaatagcg atcggagcgt tgccagagct cgacctggct cgccaggtgc
    16321 tggcgcaggg tgaagaacag gatcgccgcg caaagcgcca gttgcagggc gaaaagggca
    16381 agcacgatcc gccaccgcag cgacaggccg cgcagcatca accgccgacc agaccgcgca
    16441 ggtgggtgat gtcgtactgc gccggcggcg ccggaacgaa gccggtccag ccgaagccgg
    16501 ccagcaggct ctgtccggcg gggacgcgct ccagcccgat gaccaaatcg cgcaattggt
    16561 ccagcatcgg ctcgccgagc cgcccgtgcg ccgcaaggag gaggccgggc accgcccggg
    16621 tctggaactt gggctcgagc tcgatcggtg cgaaagcctt gagctggctc agcacgaagc
    16681 ccggcaacgc gcaggccgcc gcagaaccgc tgatgacggc atggatgcag ctcgacttcg
    16741 tccgatggta ctcgacctgc acgtcacccg gccccagcag gcgctgctcc tcgagctcgt
    16801 ggaaggcgag ctgggcgacg ccggagagct tgggcggcaa ggccagcacc cgcccgcaca
    16861 ggtccctgaa gctcgcgcat cgctcctcct tgcgggcgac gatgaaggcc gccaggtcct
    16921 cccggatccg accgagcggc cggtagtcct gagcactggc cgcgtccgcg tagaggaacg
    16981 gatgcaggaa ggcgagatcg tagcggccat ccagcagaaa ccggcgaaag gcctcgaaat
    17041 ccgtcttggt ctgcagcgcc accggccgcc cgagcatctc cgccagggca ctcgataccg
    17101 gcccgaaacg ccgcccgatc tcgagggccg gcaggaaggg aaaaacgccg aaggtcaagg
    17161 gtggctcggc cggtctttgt gggatggcgg cggccggggt ggcgctcatc gccaggctgg
    17221 cggcgagcag gcggagggca tcgcggcggg ctggagctga cctcatgcca gaacattcct
    17281 ctctgacgga cgggccaatt ggagatggat caggtatcca ctctaaaatc gaattctaac
    17341 tgcagctata gaaactttag gttgtgagat ggtcaaggtc gaccgatcgg cttcaatctg
    17401 cggcgatggt gcgatatgcc atggccatct gctatcatgc ttgtctctcg ggagtaacga
    17461 atgtcggttt cgatccgtcc gcgccggagt gtcctctaca tgcccggctc caaccggcgg
    17521 gcgctcgtca agggtcgcga gctgccggcc gatgccctga tcctcgacct cgaggacgcg
    17581 gtcgctcccg acgccaagga ggccgggcgc gaggaaatcg ccggggcgct cgccgaacgg
    17641 gagagctacg gggcgcgcga gctggtcgtg cgcgtcaatg gtctcgacac gatgtggggt
    17701 cacggcgatc tggtggcgat ggcgaaggcc ggtgcggatg ccatcctcct gccgaaggtg
    17761 gagagcgccg acatggtgcg gcgggcggtc gcggtgctcg acaatgccgg tgcgcccgcc
    17821 gatctgatgg tctggtgcat gatggagaca ccgcgcggca ttctgcaggt cgaggccatc
    17881 gccgaccacc atcgggtcgc gtgcctggtg atgggcacct ccgacctgac caaggacctg
    17941 atggcaagac acacggcgat gcgcctgccg atgctgccgt caatcggcct ctgcctgctc
    18001 gcggcgcgcg ccgcgggcat cgccatcatt gatggcgtct acctcgatct cgacgaccaa
    18061 gcgggtctgg tgacggcctg cgagcagggc cgcgatctcg ggttcgacgg caagacgctg
    18121 atccacccga agcagatcgc ggccgccaac gacaccttcg gtccgaccga cgacgccatc
    18181 cagtcggcga agcggatcat cgaggcctac gccgaggcgc tgcgggcagg caagggcgtc
    18241 accgtgctcg acggcaagct cgtcgagaac ctgcatgtgg cggaggctga acgaatcgtg
    18301 gcgctggccg aagccatcgc ggaccgcgct ggtgcctagc ccgcaggctt cgggcgcgaa
    18361 gcgtcaggcg atcaggcggt aggcgagtgt ctcgccacgc accgacatga tgcccttgac
    18421 ccagttgccg tcggaatcga accagatatc gccgctgcgc ccggtcgtgg cggcgcaggt
    18481 gaagcgatcc gccgtcaccg ggccatgggg gccctcgacg gtctcgatgc cgagcggctt
    18541 ggccgcgagg tcggtcagct tgaccttttg cgtgtcgacc agcgcctgct ggcgaacgat
    18601 ggcgacattc cagaaggcga gatcggtcat tgtgcccagt ggcacctgaa tcgcccggtt
    18661 ggcctcgcca ccctcgaccg ccaggccatt gcccgtcgcc cgaatgaagg tctcgctgac
    18721 atcaccattg tcgtcggtgc tggcccggct ctcgcgcaac tgcccgtcga tccacagatc
    18781 atcggccgtc tgcctgaact gaaaggccgt gaagaaggcg accttcacct cgatctcgat
    18841 ctcggtgcgc gcgcgaaagc cgttgtcggc aggagtgaag cggacatgat ggcggccgat
    18901 ctcgtcaccc tcgcgaaaaa tcgcgaacgt ccggttcatc ggcatcgtcg ccgccagtga
    18961 aggcatcggc aaggccaagc agccgaggcc cgcgatgcca ccaaccagaa agcgccggcg
    19021 gttgacccca tccgtgctgc ggcacgcggc ctcgccaacc ggtccaactc tcatcctgac
    19081 ttctccaaaa tcgttcgtta cagctcaggc ggtaggccgt tgtacaaatc cccgatcggg
    19141 gaccgatccg tctggcgcct gctcgatccg gtcgacccgt gccagtcgcg gcccccggtg
    19201 gcaacgctcg acgaccgcct gcaccatctc gggatcacca tgcagcaacg cctcgaccga
    19261 cccatcggcc cgattgcgcg cccacccgtc gatgcctctc gcctcggcaa tccgtatcag
    19321 ccaagctcga tagcctaccc cctgcacccg accatagata cgcaggcgtg cagccattgt
    19381 cttaaccaca gaaacctact gaccttgaaa tctggcatag cgaccgcaga acgcaatgcg
    19441 ggatcatgac actttcgttg cagtttgtgg attgaagtgg agttgtgtca cgcctgcaac
    19501 ccccgccgcc aaattgcagc gagatcctta ccttggtgca gcgaccggtc ggcagagcag
    19561 gcggtgctgt gctgccaatt gcagcccggt cggagtattg atcagacaac caatggaaat
    19621 cggaagttgc gctgttgcaa atcgaacctc cctctctagc ggccagactg gagcgcgacg
    19681 ggccactgcc gtggcgtgag gcggctggat tgacggccga gatcgcccgg ttgctcgacc
    19741 ggctgcacgc cagcggcaag gtgcacggag gcgtcgaacc gctggccgtg ctcttcgtcg
    19801 gtggcgccgt gcgtctcgcc gatcccctgc cgcccgggaa gggcgtgggg gcggacgagg
    19861 tccatgcctc ggcaacagcg ggggcatctg caccgaacgg gcgacagcat gatttcgagt
    19921 gtctcggaca gacgttcgcc ctgatgctcg gcgggatgga gaggatcgag gagttgccgg
    19981 cggcgatgcg cgccgttctg gcacggtgtg gggcgtccga tccggccacg gcattccgat
    20041 ccggcaacga aatcgccgag gcgatcgagc tggcgctgac cgctggcgag ccacctgcga
    20101 atgccggccg cctggaggcg gctgccacga gcgttcagaa ggctcgaagc gtcgagccgc
    20161 cggcctcgcc cgtgaacgcc cggcgccgcc gtccgggcgc gggtcatcgg tcgctgcttc
    20221 tgatgctgtg cggcctgctg ctgctcggtg gcgccgcgat ctttctgctg cgcccggcgc
    20281 cgcaggtcga agctccagcc gggcgggagg cgagcgggcc gaacgtgccg acgccggagg
    20341 cggtcgagga gaaagaggct ctggaggaac tgcttgccgc gctgcccgat cagccactcc
    20401 ccgaagagcc cgaccggccc actgccgaga cagtgctgcg cgagattctg gaaaacgccg
    20461 ataatcggcc gtgccagcgg ctggagatcg agcggaacgg cccggatctg cggttggtcg
    20521 ggcgaacggc cagtgcttcc gaccgggcgt cgatcatggc caggctcgac acgctggagg
    20581 aagctgtcga tacggcgatt gacatcgacg agtccaaccg cttctgcaac ctctacgaca
    20641 tcctgaagag gcgaacggcg atcgcctggc cccggctggc ccgtctctac ccgcggcgca
    20701 ccagccaccg cctgagctat ggcgaagagc tcatgatcca ggtgcagacg ccggccgagc
    20761 ccagccatct gatcgtcgac tacttcaccg ccgacggcat ggtcgtgcat cttcgcgcag
    20821 ctgccggcgg ctcaacgagg ctggcgccgt tcacggagat cgtggtcggc gatccgctcg
    20881 atggatcgtc actgcggatc ggcccgccat cggggaacga ggtgattctc gtgctcgcaa
    20941 gcacggcgcc gctcttttcc gcagcacgcc caccaacgga gaaggccgat acctatctgg
    21001 ctgcgctcga cgcggcgctc ggtcagacgg cggcaaggcc catcgcctcg actctgacga
    21061 tcgagaccgt gccctcggac aattgagcag gttccgtgtg ctgtccgatg cggtaatggc
    21121 ttgaaaaaac aggtagaaat agatatagta tttttcaacc tttggctgtt cgcgaaacgc
    21181 tgatgacgga cgaggaagag caagatcagc gagccagcct caatctcgct ttcgcgatcg
    21241 gcgggatgaa ccatttcagg gtcgacgtgg cctccggtct gattgctggt ggtccgctgg
    21301 tgaagcgtct gctcggtgag ccggatgccg gcgaagcgtt cagggtcgag cggctgctca
    21361 ccaggctcga cgcaacgact ggccggcagc tcgcccaaat tctcaccgac ttgccgcgtc
    21421 acgacctggc cgtggatgtg gagcttgttg tcgtcgatca cgcagcgatc gacgccatca
    21481 ggatggtcgg acgagcccgg accatcccga caccgatcct cgccggaatc atggttgacg
    21541 aaacggagcg aaggcgcagc gaggagctcc tgcgaggacg tgctgccgac ctcgcagccg
    21601 cgaaccgcca gctcgagcag ttcacccaca tcgtcagcca cgatctcaag tcgccgttgc
    21661 gcggtgtcca tcatctggcg agcttcctgc gggaggacct gggagacgcg gccagcccgc
    21721 aggtgttgga gcatctggac aagctcgacc gtcaggtgac caagatgagc cggatgctcg
    21781 atgatctgca gacctacgtg catgtcgatc gacgggccgg gaagatcgag accgtggacc
    21841 tcgaagcact gggcgtggag ctcggcgagc tggtcggact gccggacggc tttacgctcg
    21901 atgtcgatgt cgagcctgtc cgggtcgaga cagatcgaac cgccctgtcg ctggtgctgc
    21961 gcaatctgct cgacaatagc ttcaagcatc atgaccgaaa gtccgggatg ctgcgcatcg
    22021 aggcccggcc ccatggcagg ggacggcaaa ggttcagggt gatcgacgac ggaccgggca
    22081 tcgttcgacg ctatcgcaag cgtgtgttcg agccttttca ctgtctcggc ggggcgggtc
    22141 ccggctcggg gatgggtctc gccttcgtca agcgagccgt ggagcgtcag ggcggccgcg
    22201 tctggataga ggacgggccc ggggccgatg ccgggcgggg agtggccttc tgcttcacct
    22261 ggccgctggt cggatgtggc aagccggatg gctgacggcc cgggcacgca tatcctgctc
    22321 gtcgacgacg acgagatcga cgtgatcgca ctcacccgcg cctttgccga ggtgggtatc
    22381 gatgttccct tgatcatcgc ccgggatggc gaggaggcgc tcggggcgtt gctcgagcgt
    22441 gggctcgacg cggtgctgat gttgctcgac ctcaacatgc cgcggatggg tggcttcgag
    22501 ctgctcgagc gtcgggcgag cgacccccgc ctcggtgcgg cggccgtgct cgtgatgacg
    22561 acctcctcgc gccgccagga catcgagcgg gcgcacgctc ttggcatcgt cggctacgtg
    22621 gtgaaggcga cccgccccgg gttcagtcgc gacttcgccg agctcgtccg tcgctatctg
    22681 gcgctggcaa gcctgcctcg cgcggggggc agaacccgct gatcccggcc gctgccgcgc
    22741 tcagctggcc accggctggt tggccttgcg gtactccgcg aagagcacgg ccagcgccgc
    22801 cgatgccatg cgggcccgga gcgcgtccgc gcgacttgtc agcatgatcg gcacccgcgc
    22861 gccgagcacg acgccggccg cgtcggcatt ggcgaggaac gtcaggttct tggccagcat
    22921 gttgccggcc tcgagatcgg ggacgaccag gatgttggcc tggccggcga caggcgagac
    22981 aatgcccttg atcctcgccg cctccgggct gatcgcattg tccagggcca gcggcccttc
    23041 gagaagtccg ccggtgatct ggccccggtc ggccatcttg cacaatgcgg cggcatcgat
    23101 ggtcgatggg atcttgctgg tcaccgtctc gactgccgag aggatggcca ccttcgcctc
    23161 gagctgcagg gcgatggcca gatcgatggc attttgcacg atgtcccgct tggcatcgag
    23221 gtcgggaaag atgttgaccg cggcatcggt gatgaagagc ggctgcgcgt aggtcgggac
    23281 cgccatcgcg aagacatgac tgatgcgccg ctcggttcgc aggccggtgt cgcgatgcgt
    23341 gacctccgcc atcagctcgt cggtgtgcag gctgcccttc atcagaagct cgcccctgcc
    23401 ggctcggatg agcttcaccg cctcggccgc cgaggcatgg ctgtgcgggg tgtcgacgat
    23461 ctcgagcccc ttcagcgacc tgccgagctc cgcggccgcc gcctcgatgc gggagcgcgg
    23521 gccgacgagc agcggctcga tgatgccgct ggcgcgagcc tcgagtgcgc cttcgagtga
    23581 caccgtatcg catggatggg cgacgacggt cggcgtcggc gagagccgcc gggtcgtctc
    23641 gatcaaccgc tcatacgctt cgtgccgcgg gccgtccttg ggtgatgtca tcgttccgct
    23701 ttcctgcatg gcctgccgac tgtgtgcttc gcctatcagg gaacgcgggc tcgagaaaga
    23761 tgcgctcttg ccgcaatgca gcgtgccgaa ttccggagcc gcgtcgccgg cgcaacgtga
    23821 tggatcgccc cgccacgatc gtctaagacg aaacccgggt accggtctcg agcagcctcc
    23881 aacggaaggg ttcagccatg cgcctccatg atttcgggtt cctgatcatc gccgcgggca
    23941 tatgcgcggc cgtcgccttg atcgtggccg cacaagttgc ctgagtgagc cacgctgccc
    24001 gacatcgttt ttcacggacg cgatgaccgg atttgggtcg gctgccaatg acgccggtgg
    24061 atgccgccca gctggagcat cgatattcgt ctcgcacggc gacgctcggc atcatgggca
    24121 tgggctatgt cggcctgccc ttgatgctgg cggctaccgg agccgggttc cgcgtgatcg
    24181 gcttcgacat cgacctgccg aaggtcgaag ggttgaacgc cggcctgagc ccgcttgccc
    24241 atatcggtca tgcgcccatc gccgcggcaa gggaatcggg tcgcttcgag gcgacgggtg
    24301 atttcggtcg catcggtgaa accgatgcca tcctgatctg cgtgccgaca ccgctcggcc
    24361 ggcacaagga gcccgatctg tcctatgtgg tcgggacgat cgaggcgatg gcaccccatc
    24421 tgcagccggg ccagctgatc gtgctcgaat cgaccacctg gccgggaacc accgacgaga
    24481 tcgtcagacc gctcgtcgag gcggcaggtt tccattccgg ctcggacatc ttcatcgcct
    24541 acagccccga gcgcgaggat cccggcaatt cggagttccg gaccggcacg atcccgaagg
    24601 tcgtcggtgg tgacggcgag accgcgctca ggctggcctg cacgctctat cgccagttcg
    24661 tcagccatgt cgtgccggta tcgacccccg ccacggccga ggcggtcaag ctcaccgaga
    24721 acatctttcg ggcggtcaat atcgccctgg tcaacgagct gaagctggtc ctcgagcgca
    24781 tggatatcga cattttcgag gtcatcgacg ccgcgaagac caagcccttc gggtatatgc
    24841 ccttctatcc cggacccggg ctgggcggcc actgcatccc gatcgacccc ttctacctga
    24901 cctggcgggc acgcgagttc ggcatcgcca cgaagttcat cgagctggcc ggcgagatca
    24961 acaccgcgat gcctcaatac gtcctggggc gagcggcgga ggccctcgac cggcagcagg
    25021 gcaagggcct ttccggcgcc acggttctgc tgctcggcgt ggcttacaag aagaacatcg
    25081 acgacatgcg cgagagcccc gcgctggcaa tctgggaatt gctgcgcgat cgggatgcaa
    25141 cggtcaccta tcacgacccg aacgtgccgg tcatacccgc gacgcgcgag cacgcctcgc
    25201 tcaccggctc ccggtcggtg ccgctcgaca gggcggtggt cggggcggtc gatctcgtgg
    25261 tcatcgtcac ggaccatggt tccgtcgact attcgctcgt ggcggaagcg gcgcggctgg
    25321 tggtcgacac ccgcaacgtc atggctccgt ttgcggccag gatggggtca cgactggcac
    25381 gcgcctgagc ggcaggcgcc cttgttgcgg gtcgagtttc acggcatacg atgccgccat
    25441 cgactgctcc tggaggatgc caatggcgct catcgcccga agtctggctc gcatcaagcc
    25501 gtcaccgacg atcgccgtga ccaatctggc gcgggagctg aaggcggccg gtcgcgacat
    25561 catcggcctc ggcgccggcg agccggactt cgacacgccg gagcacatca aggaggcggc
    25621 gatcgaggcg attcgcgccg gcgacacgaa gtacacggcg gtcgacggca cgcccgcgct
    25681 gaaggaggcg atcatcgcca agttcgcgcg cgagaacagc ctcgtctacg gcatgcagga
    25741 aatctcggtc ggcaccggcg ggaagcaggt gctgtacaac gctttcatgg cgaccctcga
    25801 tcccgaggac gaggtgctga tcccggcacc ctattgggtc tcctatcccg acatggtgct
    25861 gctcgccggc ggcaagccgg tggcgatcga ctgcactgcg gaggcggcgt tcaagctgca
    25921 gcccgatcag ctcgaggccg cgatcacgcc ggcgaccaag tggctgatcc tgaactcacc
    25981 gagcaacccc actggcgccg cctatacgga aggcgagctc aaggcgctcg cggacgtgct
    26041 gctgcgccat ccacaggtgc atgtcatgac cgacgacatg tacgagcatc ttgtctacga
    26101 cgatttccgc ttcacgacga tcgcgcaggt cgagccggcc ctgcaggcgc ggacgctgac
    26161 ggtgaacggc gtctcgaagg cctatgcgat gaccggatgg cggatcggct atgcgggtgg
    26221 cccggccgcg atcatcaagg ccatgggcat ggtgcagtcg cagagcacgt cgaacccgag
    26281 ctcgatcagc caggcagccg cagtggcagc gctcaatggc ccgcaggatt tcctccccga
    26341 gcgcaatgcc gtgttcgccc agcgtcgtga tctcatcgtc gacctgctca acgaggcgcc
    26401 ggggctgagc tgcccgtcac cggagggtgc cttctatgtc tatccgagct gcgccggcgt
    26461 gatcggccgc aagtcgccgg acggggccgt catcgattcg agcgaggcat tcgcccgatt
    26521 ccttctggag gatggtggtg tcgccgtggt gcatgggtcg gccttcggcc tcgatccgca
    26581 tttccgcatt tcctatgcga cggcgaccga gcagctcgag gaagccggca agcggatcat
    26641 cgccacctgc cagaagctgg gctgaacgcc agccgtctat cggccgaaat gtggatggtg
    26701 aacgacgtcg ccgacggcga ttcccagacg ctcggcctcg ccaccgcgga tttcgagcac
    26761 gcccgcgacc gggccgcccg aggcgagcac ggcctccgag tacggggtcg tgcgggcgtc
    26821 gatcttgcgg atgaggccgg tggcgtcgat gaacagcatg tccaggctgg tcggcgtgtt
    26881 cttcatccac atggctacgg gacgcggcgg ctcgtggaca aaaagcatcc cggtgccgtc
    26941 cggaacatcg acgcgaaaca tgagaccctt gctctgctcg gcgggagaac gggccatctc
    27001 gaccaggaag tgaacagtcc ccgccttcgt gcgaatggcg agcggctcga tcgcgagacg
    27061 ttcctgtggc gtgccacccg cgtcctgggc ggcactgccg gaaacccatg cggcaggcag
    27121 cagcgccata accccgagga cgccgcgccg cccgaagctc cgcttgccgc tcatgggatg
    27181 aactgctcct tcaagatccg ttcctccagg ttgtgctcgg ggtcgaacaa gagccgaaat
    27241 ccatgggtct tgtcttccca gatttcgatg gaagtcacgt cgcgcacctc ggtgaagtcg
    27301 gcgaccgcgc tgaccggacg cttgatcggg tcgagaacgc ggatacgaaa tcgcgttcga
    27361 ctgccgagca gcgcaccacg ccagcggcgg ggacggaagg cgctgatcgg cgtgagcgcc
    27421 aggatattgg agcccagggg aatgatcggc ccgtgcgcgg agaggttgta agcggtcgag
    27481 cccgccgggg tcgccaccat gacaccgtcg cagaccagct ccggcaatcg tttgacgctg
    27541 tcgatctcga tgctgagagc cgctgcctgg cgggtctggc ggaagaccga gacctcgttg
    27601 atggcgaggc cgcgcacctc gccgccactc gcccgttcgg cgcacatgcg aagagggtag
    27661 agatccacgg ccattgcccc ggcgatgcga ccgaggagat cgtccggcgt gtaggcattc
    27721 aggaggaagc cgaccgtgcc gaggttcatg ccgtagatcg agacgtcgcg atcgatgaag
    27781 cgatgcagca cttccagcat gaatccgtct ccgccgagcg ccacgaccac gtcggcggaa
    27841 tccgcttcca ccgatgggta acgggccgtc agatcgtgga gtgcacgccc ggcatccggc
    27901 gtgtcggcgg cgaggaaccc gagcctcatg cgatgcgagc ccttccgggt gagcagctgg
    27961 cgaaaatgcc gtcggcggcg tccgcgcgga agctggtcca ttcacaagcg gggtcgagcg
    28021 tattagcttt agggagaatg cgtaagccgg gaatgccagc ggcttggcgc tcgaggtgga
    28081 ggacagcatg ctcgtcgacg gcttcggccg gcagatttcc tatgtcaggg tgtcggttac
    28141 cgaccgctgc gatttccgtt gcgtctactg catggccgag aacatggtct tcctgccgaa
    28201 gcgggatctc ctcagtctcg aggagctgga aaggctgtgt gatgccttca tccagaccgg
    28261 tgtccgcaag atccggctga ccggtggcga gcctctggtg cggcgtggcg tgatgtcgct
    28321 gatacaggcg ctgggccgcc ggttgggccc ggtgcccgga accctggagg agctgacgct
    28381 gacgaccaac ggcagccagc tcgagcgcca tgctgaggga ctggtcgccg ccggcgtccg
    28441 ccgcatcaat gtttcgctcg atacgctcag acccggtcgc ttcgaggcga tcacccgctg
    28501 gggccggttc gaacaggtga tgcagggcat cgccgctgcg cgttcggcgg ggctcgcgat
    28561 caagatcaac atggtggcgc tcaaggatgt caacgaggac gagttcgatc agatgatcgc
    28621 ctggtgcggt gccgagggct acgaccttac cctgatcgaa accatgccgc tcggcgacat
    28681 cgatggcgac cgcaccgatc agtatctgcc gctgtcgttg gtccgcgccc gcctgcagca
    28741 acgctggagg ctcaccgatc tcgactgctc gaccggtggg ccggcgcgct atgtgagggt
    28801 cgaggagacc gggggacggc tcggcttcat cacgccgatg agccataatt tctgcgagtc
    28861 ctgcaaccgc gtccgggtca cctgcacggg tacgatgtac atgtgcctcg gccaggagga
    28921 tgcgatcgac ctccgcacac ccttgcgcgg tgccgaggac aacggcccgg tggtcgccgc
    28981 catcgaggcc ggcatcgggc gcaagcccaa gggtcatgat ttcgtcatcg accggcgccg
    29041 caagcgcccg gcgctggcgc ggcacatgag cgtgacgggc ggctgacccc tccgggccgg
    29101 ccctcggtct attcgatctg gcgcccggcc agcgtcatct gccggctccc cagcacgagc
    29161 ttgtgggctt cttccgccgg aatcggccga ctgaacagat agccttgcag ctcgtggcag
    29221 ccgcagccgc gcagaatctc gtactgctcg ggcgactcga cgccttcggc gatgatcttg
    29281 aggccgagag cggagccgaa ggcgatgatc gcgcgggcga tcgcggcact tgcttcattg
    29341 tgcgcgatgt cccgcacgaa ggaacggtcg accttcagcg agcggatggg aaatcgcttg
    29401 agataggaga gcgacgagta gcccgtgccg aagtcgtcga cggcgaggct gatcccgagc
    29461 tcgtcgagct cgcccagccg acgcgtggca tcgatcacat cctccatgat gacgctctcg
    29521 gtgagctcga actcgagcag acgcgccggg aaccgtgtcg cctcgagggt ctggcggaca
    29581 tgcggcacga gattgcggtt gttgtactgc cgcccggaga tattgatggc cagtcgcaag
    29641 ggggcgatgc cggcatcgaa ccatgtctga agctcgcggc aggcggcctc gaggacccat
    29701 tcgccgatgg cgatgatggc accggtctcc tcggcaagcg ggatgaactc cccgggcggg
    29761 atcatgccca gctccggatg ctgccagcgg atcaacgcct ccatgccgac aatgcgcgac
    29821 gaggcgaagc tgatctgggg ctggtagtgg aggaggaatt caccccgctc caccgcttcg
    29881 accagctgtc gctccagcat caggcgatcg aaggagacgg cgttcagcgc attgctgaag
    29941 aactggaaga cctggccgcg ccctgccggc agccggtcga gtgccgcttc ggcgttggcg
    30001 agcagcgttt ccgcctgatc gccgtcgttc gggaacaccg cgatgccgac catgccgccg
    30061 acggcgatct cccgaccgtc caccacgacg ggctccagca gctcgtccag catcacacga
    30121 gccagcttgg cagcgccgtc ggcgccctca tgaccgaagg cgagaattgc aaagcgcatc
    30181 tcgtcggttc gcgccagggt gtcgagacgg ccgattgact gctcgaggcg gcgcacaagg
    30241 gccgtcagga gctggtcgat ggctgctcga ccgaaggagc gctcgacgag attcaggcga
    30301 tcgataccga ggaccatgac cgtcaagccg gagccagcaa gcgtcgcccg tgccacggcc
    30361 tgccgcaggc ggtcgagaaa caggaagcgg ttgggcaggg ccgtggccgg atcgtgatag
    30421 agcgtgtcgc gcagcgagcg gccggaagag ccctcgccag cgagcgtcac gagcacatga
    30481 cccccgccgc agatcgacag tcgctgcagc acaaaccgcg gcgtcagatg cgccggagcg
    30541 ccgacgggca ccgtcagcga tggcgcggcc gccggatcct tcccgccgcg gacgaactgc
    30601 tcgaccgcaa ccgagtccgg cagcgacaaa caccggatcg attgtccgat gatcgtttcg
    30661 gtcgactgac cgagcgccgc gccgaaatca ccggagcaac cgacgacatg gccggtcggg
    30721 tcgaggagga tcgatgagac acccagaagc ccgagggctt cgaccagagc cgactgcgcg
    30781 cttgtgctgc cgagcgtcgc gggcagcgtg gcaaaggacg gctcgatcgc accaggaggc
    30841 cgtggatccg gcagcggatc gacgatccat gcacagccct ggaggccgac gacctcgcca
    30901 cttgctggat gccgcacggg aaccgccgtg tcatgcagcg tgatccggct cctggccgcg
    30961 tggcacagcc gataggtcga cgactgaggt acgccttgca ggagctccag atgacgccgg
    31021 cgaagggccg ggcgggccgc ccgctcggcg aggtgcagcc agccctcggc ggtgtctgcg
    31081 agccccagca cccggctgaa tctcggttgg ctcgcgacta tccgccactt tccgtcgggt
    31141 gccaggacgg cgaagtagcg aaacgagcca ggtggcggcg gcagcaaggc ggttccaggc
    31201 caggcacgcg cgagcgtgtc cgtatccgag accagctccg cggtcatgag gtaggcgggg
    31261 tcggccgggc gaccgtgcag ccgcacgact gccggaccat cgcaatcgag cgctatctcg
    31321 cgatcgaagt cgcgccgttc ggcgatgtgg gccattgcat cagcatgatc ggcggccgcc
    31381 acgaagcagg tttcgccctg ggaggggcct gcgtcacccg taagctcgcc atcctgccag
    31441 agcgccagcc aggccgcatt ggcagcgacc acgcggacgc tggccggtga tggaccactg
    31501 ctgtccagga gagcggccgc gatgggcaag gcatcgagca gcggctgagc cggcagcacc
    31561 tggctcgaca gttcagacac cagcgacacg caatggtccg gctcgagggg agatcatcgc
    31621 agcttcggaa ggcggccgat ccagcagcat gaaactactt ataggagaaa ataattgcgc
    31681 atattagcct taagcgcaag tagagtccgt ttgtccgcca cgccgatgcc atgcatggat
    31741 tcccggcacg gcgccgctat gttgtccggc ggttcaatca cctgacagct cgcccaggat
    31801 ttgcgggatc cagcccgcga gatcggtggc gagcatcggt gttgcgcatc gctcggcggc
    31861 ccgggcatgc agccatgcgg cggccgccgc cgcatcatag gcaggcatgc cctgggccat
    31921 caggccgacg accagaccgg ccaggacatc accggtgccg gcggtggcga gcttggccgg
    31981 tgcctgggca ttgatgcggg cgccgccatc gggtgtcgcg atgaccgtgt cggcgccctt
    32041 gagcaggacg atgcagccgg cgcatgccgc cgctcgccgg gcgcggtcga gacggtcgcc
    32101 ggtgaaccgg aagagccgtg cgaactcgcc gtcatgcggg gtgagaacgg tgcttgccgg
    32161 cagcttccgc tcatcgatcg aaagatgcga gtcgccgagc gcggtcaacg catcggcgtc
    32221 gaggacgcag ggcagacggg cgtcaagcac gcgctcgacc acggcgaggc tgtgcctgcc
    32281 gagcccgaag gccgggccca ccagcatggc ggtgaagcgg cgatcctcga ggagccgtcc
    32341 gagatctgcc acgttctcca tcgggcgggt gagcagggtc ggtgcatggc tcgcgtagat
    32401 cggcagcgcc tcgcgcggtg ccgcaacggt gacgaggccc gccccgaccc gtgcggcggc
    32461 gatcgccgcc atgctcgcgg cgccggtcga ggtcgcgccg ccacccagca ccagcacgtg
    32521 gccatggctg tacttgtgct cctgcgccgc ccatcgcggc agcatggcgc cccactcggc
    32581 gggtccgttg acgcgcaggc cggtgtcgtg tgcagcgacc gcagcctcgg gtatgccgat
    32641 atcgacgcag cggatttcgc ccatgaagga gcgggccggc tccagcagat gggccggctt
    32701 caggcaggca aaggtgacgg tcaggtcggc gggcagcgtg tcgggtgaaa ccgtgcccgt
    32761 cgtgccgtcg accccggacg gcacgtcgac cgctaccacc tgcggccgcc cggctgccag
    32821 ctcgtgcagt cgcgcgccga gggccgggtc gagtggtcgg tcgaggccag ccccgaagag
    32881 cgcgtccacc aggatgtcga acgagccggc ggccggttcg gtcagccgcc cgatcggccc
    32941 gttccagcgg gcggcggcaa gcgccgcgtc acctttcagt cgcgcgggat cgccgagggc
    33001 ggcgacccgg accggccacc cggccgccgc cagtcggcgg gcgatgacat agccgtcgcc
    33061 gccgttgttg ccggggccgc agagcaagag gacacgacct ggcgacagcc gcccggtgat
    33121 ggctcgaacc accccggctc cggcgttctc catgagccgc gcgccggaga tgccggctgc
    33181 gatcgccgcg cgatcgacct gcgccatggc ttgtgtcgac agaaccgttc cgcagagcat
    33241 ggcaaactcc aggcgcgaga gcctgccagc cgcggcgttg cgggccgcgt taggcaagcg
    33301 ttaaggcttc gatgatagtc cgggcagccg ttgccggcga caggctcgcc ggcgtcaggc
    33361 agcaggacga tggctgatga gcgcactttt cgacttcgcc cggctggcgt cgttcgccga
    33421 ggatgaccgg acactcgagc aggagctgtg cggcatgttc ctcgtgaccg ccgatcgcta
    33481 tctcgacgac atgcatcggg cgatgggatg cgccgaaacc tgggcggcgg aggcgcatcg
    33541 tctcaagggg gcggcggcca atatcggtgc cgatgccgtg gctgagctcg cgctggccgc
    33601 cgagcacggc cagccggacc cggggcggct ggcccaactg caggacgcac tggcagcgac
    33661 gcgacaactg ctggagaaac atctcgggcg aggctgatcg gtgccgtttc gcccggcaat
    33721 ccgccggtga atgcgccgtc ctgattgccc gtcttgtcag cggcatttgc ggtctgatcg
    33781 acctgacgtt ggatcgtggc aacaccgcag gttcaacatc tcgacgcgcc agaattccct
    33841 tgattgagcc gtccatttcg actgatatcg cgccagtttt ttcggggtga ctcctgaagt
    33901 ccatcgggcc aacgcccgta actgcggcga tatcctcatc ggttcagagg ggcgatgtga
    33961 tgcgaaatgg ggctagattg ctcgcggcgg gcgccttgtg cagtgcgttg gtcgcctgcg
    34021 gcggcatcga tccgaatatt cacgacgatg ccgacctcta cgcctacgac atcgacgatg
    34081 ttcggagcat ggccccgcaa ggtgctccct tcacccaagg gttgcgcagt ggttatctgt
    34141 ccttggcgga ttccgagacc gtggcgatgg gattggccaa ttccaggcat ttccagcgca
    34201 aggcggtcgg ctcggccaag gggcaggcgg ttcagccgga cttcgtcagc ctgcgcaccc
    34261 tgagcaccgg gcagatcgac gagctgaccg cggcacgggc ccggctgatg gcgggactgg
    34321 acggcggtgg ccggcagaag gcgccggcgg cggcggcccg ggcgcagacc tcgttcgatt
    34381 gctggctggc cttcgaggaa agcaacgacg cggccgggat ctcggcctgc aagaaggctt
    34441 tcgaggacgc catggccgag gtcgagcgtg ccctcgcgac cgatatcggc aacgtctatc
    34501 tcgtcttctt cgcctgggac cgcgccgacg tcacgccggt cgccgcgacg atcctcgacc
    34561 aggtggcgca ggactacaag gacggcgaaa ttcccagggt cgtgctggcg ggccataccg
    34621 atacctcggg cacggcggac tacaacctgc gcctgtcgga gcgccgggcg cgggccattg
    34681 ccgcggagct ggtggcgcgc ggcgttccgg aaacggcgct cgaggtcacc tggttcggcg
    34741 agaccgagct gcgtgtcccg accgcggatg gcgtgcgcga gccgcagaac cggcgcgtcg
    34801 agatcctctt cgccgactag gagcctgtcc gagaagggat cacgacctgc acgcgccagc
    34861 cctggctctg ggccacgtcg accggtcctg acgatggccc cgcatcgcgg caggccggtt
    34921 tcctcgccca aagccggggt tggcgcgtgc aggtcatggc catttcccgg acaggctcct
    34981 agacgggccg accgcaagga ccgaccagac ggatggtggc cgactgacgg ccaccgatcg
    35041 gacgaacgtg caacgaaggg cggccggaag gtcgcccttc ggcattcggc ggggccggcg
    35101 cgcccaatgc ctcgccggac atctgatgac cgcgcccact tgcccgtcga cccgaaggat
    35161 tggatattgg ccatatgcga tacgtgctct ggacattgct ggcggtgctc gtcctggtca
    35221 tcggcgggtt tctgagctgg accctgccgt cgcgggaca
//
LOCUS       DAOTFI010000010        34853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1360_length_34853_cov_7.383039, whole genome shotgun sequence.
ACCESSION   DAOTFI010000010 DAOTFI010000000
VERSION     DAOTFI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 34853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34853
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1360_length_34853_cov_7.383039"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="P5558_01720"
     CDS             <1..1321
                     /locus_tag="P5558_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY23059.1"
                     /translation="ELEAERRAQFLSRHDALTGLPNRNMLAERLDETIAQVARRREMA
                     AVLCVDLDRFKDVNDTLGHGAGDALIRQVAERLEACLRETDTLARVGGDEFVVIQTAV
                     QDVAAVELLARRILSVVEMPFDIGVERVLVTASIGIAMLPGDGAISGRLLQNADIALD
                     RAKSEGRSTFRFFERDMDARLQARKALERDLRLAIVRQELELFYQPKIDLKKGRVSGV
                     EALVRWNHPERGMVSPADFIGVAEETGLIVELGAWVLRRACTDIRGHRDIELAVNLSP
                     VQLKRGGIAEMVADIIAETGFDPDRLELEITESVMIEGAEAAIAELRHVKDMGARIAM
                     DDFGTGYSSLSYLNRFPFDKIKIDRSFVMDLGKRGNGAAIVKAVVGIGESLGMTICAE
                     GVETIEQRRFLEQAGCHEAQGYYFAKPMPLAQLTGFLAEPTTVLAPA"
     gene            1473..2081
                     /locus_tag="P5558_01725"
     CDS             1473..2081
                     /locus_tag="P5558_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRY23060.1"
                     /translation="MSDVLSRLKKLTKCLHIYLAETPGRGFGIFAARAFAQGSTLVVD
                     EDGDYYAGEMTLAEAQARGYDLSQDLFQIGTDAFLLPNANLDDFVNHSCDPNTGLRLT
                     PAGYRMIALRAIAQGDELTYDYSTYISGTPERMSCACGAANCRGTIGAFETLPPALQD
                     RYLAAGVVGRFVAAAARNAAIQDAARAESGAGVRAGGEAVSA"
     gene            2122..5328
                     /locus_tag="P5558_01730"
     CDS             2122..5328
                     /locus_tag="P5558_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY23061.1"
                     /translation="MAASGGLRGSVVVRLALVAGLLSAGKVLLYTAGTTLFLARDGIA
                     GLPLLYVTLAVLATLVSIGLGHIVDRVPGGRLLPGLALAIAVVVAGLLGGLVLGLAWA
                     PMGLLLAAHLYDIVTDIVFWVLVAAFLDNLRLRRLTTRLYIAIAIGGSTAGILAERAL
                     VWLSPGQLLLPVILLAGLAALALWPVAVPPTTGAGGEAGEVGTGLGDLGRFLARHPFA
                     LLLALNSMMLTVVYSLTEFVCFALYAETYADEAELGRFLALIFAGLQLAELAVLWLAA
                     RGVVERAGPFLRNLLFPLGSLACLAALLAQPRLAWAILAHVNTEAVSNAVFEPVNTTN
                     YGALPAAVHGRARTLADGVFYPVGMALAGLVLAILPADDAAFRARVLALVAAGFFVLL
                     NLVVAQRYLPTLVRHLRHGQRPRLPAAPRRRAASGDPAIDPARLLPLLASRRAADRLL
                     AVDLVEAALARAQPEAAEALVAALATALVGVAPRLGETDWARVVAILARAGPDALVPA
                     LEAATARTEREAMSLLGAACLLAGGAPRVPVAGDVALALADLLGGCPWMHPNSTADQG
                     SPGWRNDAQLIGRLAAVVRRAPPALTQHLAQGIDHAETPEELRQALLEAVLRHPPKTP
                     DPALLVLAEEGRHAAAPAHRAMALGALAAMLPPAELLGPGMAALTDPSRQVRRVAVDA
                     LLPLADRALAVLLVDGGSRGAASLGVIELLAAVDSRPARRLLRRLIAGLAELAERDAR
                     LLDRIAAAGDTSLAPLHAALLDHARSLTDRLFAGLGALGEAGHAMELRRALGEADTRL
                     VAGGIDGLVSLRHGQLARRFVPLLERLHLPHQGAAADQLHVRAGASADTAIERLVEDI
                     DGRWLRAATRLLRRRRADAPAAPDLPTPTDEVAMPIFAPPARSTHDDRLFEAMLRLKR
                     FDLLAELPLDALEVLAPLVEERRFAAGMTIQRGGGVLPLAWLVDEGSVEVSWPDGRIE
                     TEGPGGIIGETALVDPTVTAPGVTAGTACRLLQLHRITFEDIARDHPALTEALCRLLA
                     RRLREARLHRGRQPDTPTTQMARQPESRRETASAVGDR"
     gene            5484..6086
                     /locus_tag="P5558_01735"
     CDS             5484..6086
                     /locus_tag="P5558_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HRY23062.1"
                     /translation="MSDDDAPSLAELTTRIVTAYFRSHDLAAADVGRLVTTVGTELGS
                     LGRPPEQPAAAEPAVPIEDSVRRDGLVCLVCGRRMKTLRRHLLSAHGLTAGDYRERYA
                     LDRDYPMVAAASSARRAEIARRTGLGLRTAVEPVPAPAVPVPQAPEPAPEQPKRRLGV
                     KPIRGRRAAKPAGTEDPARQPQPEPAPEPKRPPGAGGSST"
     gene            complement(6083..7951)
                     /locus_tag="P5558_01740"
     CDS             complement(6083..7951)
                     /locus_tag="P5558_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23063.1"
                     /translation="MRVPHVAGRPAAPAQAARPPHAVVGSQIDNEEEIFAAFDGRILR
                     RFWEFVSPYSRRLLLALAAVLAFALSQVAIPLILRAVVDSALAGETADAAMAAERLLL
                     VGVAAFFAVVSLNFVANLVQETLVARIAERLLVDLRRAMFAHLQRVSLSFMDKTEVGR
                     LMSRLQGDVHALQEFLETSVFAIGDLVLLVGITVVLLVLDWRLGLLTLSVMPVLVIVR
                     LIWLPRARRAFLRARVTSSTLNGALAEGIHGVRTVQGMGREAVNLRLFEEKAADNLDA
                     QLHGTRLAQLMVPIVDTLTGGAMGIVVVFGGMAVLDGALDLGVMVAFLFYVQRFFDPI
                     RSLTMQYSVMQRAMASGQRIFEVLDVPIAVVDRPDALDPETIDGSVVFEDVTFGYVKG
                     VPVLRNVSFEARPGETVALVGPTGSGKSSTMALAHRFYDVGGGRVLVGGHDVRDLTQA
                     SLGRHIAMVLQEPFLFTGTVLENIAYATPGATRDAVEAAARAVGAHDFIMALPEGYDT
                     PLEQQGGNLSVGQRQLISFARALVADASILILDEATANVDSYTERLIQQALVRLLEGR
                     TALVIAHRLATIRHADRIVVLRDGQVIETGSHAELMQENGLYARLYRLNYASFDDV"
     gene            complement(7948..9852)
                     /locus_tag="P5558_01745"
     CDS             complement(7948..9852)
                     /locus_tag="P5558_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23064.1"
                     /translation="MPASDPLITRSSPPARVTVGLSDIGILRRVLGMALRYRRGMAIA
                     LVTTLLAAVFQLLIPQYLGKAVDSARGLLAGGDAEAARAALWRTAFLLLGASVLRGLF
                     TMWHNYQGEAIGQRIAYDLRMAYYAQLQRLSFDFHDRTHSGELITRGMLDIEGIRMVI
                     NAGILRLVLITVLVGVGATLLLRQDLWLGLIALSFVPFAAWRSVVARLRLRAGWLALQ
                     EKLGILTRVMEENLQGIRVVRAFAAQPHELGKFDAISEETLALADHRIDIRVRNTAAM
                     TYAFFLAMGLLLWVGGGKVLAGEISVGTLAEFLAFMAILQMPVRQLGMMVNAFARASM
                     SGGRLFEVLDREPEIQDAPDAKELRITDTALRFENVSFTYAGARTGARASGGGPTLAT
                     LHDISFEVRAGRTLGIVGPPGSGKSTIAHLIPRYYDVGGGRITIDGQDVRDVTLGSLR
                     RAVSLVQQDTFLFTASIENNVAYGNPWAERDRIAHSSEMARLHDYIAGLPKDYETLVG
                     ERGVSLSGGQRQRLSIARSILVDAPILIFDDSTAAVDAATEQGIREALRSQSTRRATI
                     IIAHRLGSLMDADEIVFLEGGRIVERGSHQELLALGGRYASLYALQSLGNEPVAGAPA
                     APSPVAEPER"
     gene            complement(10058..10134)
                     /locus_tag="P5558_01750"
     tRNA            complement(10058..10134)
                     /locus_tag="P5558_01750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10098..10100),aa:Pro,seq:ggg)
     gene            complement(10186..10710)
                     /locus_tag="P5558_01755"
     CDS             complement(10186..10710)
                     /locus_tag="P5558_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRY23065.1"
                     /translation="MVVTADQAAIPAQTRGKSQAAFRTISEVADELDVAQHVLRFWES
                     KFPQVRPLKRGGGRRYYRPEDIDLLREIRALLYDEGYTIKGAQKLLRQRRARPPAIAR
                     TTEKPSPPAPAPPLAATDAVPDPAAAEPTSNAAPPPAASATAAPDRARLHHLRDSLVS
                     LREELAQALAEADS"
     gene            complement(10712..11017)
                     /locus_tag="P5558_01760"
     CDS             complement(10712..11017)
                     /locus_tag="P5558_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRY23066.1"
                     /translation="MTGRTVTRAQLAEAVYQEVGLSRSESAGLVDAILEEIAHSLVMN
                     GTVKISSFGTFAVREKGERVGRNPKTGEEVPILPRRVLVFRASQVLKSRINSERPAA"
     gene            complement(11124..12095)
                     /locus_tag="P5558_01765"
     CDS             complement(11124..12095)
                     /locus_tag="P5558_01765"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRY23067.1"
                     /translation="MRRSILRAVGGYLPDRIVTNDDLARTLDTSDDWIQERTGIRRRH
                     IAAPDELTSDLAVAAARRALARADLGPDRVDLIIVATSTPDHTFPATATAVQRKLGCG
                     PGIAFDLQAVCAGFAYALATADNFIKAGQASTALVIGAETFSRLLDWTDRGTAVLFGD
                     GAGAVVLTALEQPGTALDQGILATMLAADGRHYAHLQVDGGPSSTGTVGHLRMNGREV
                     FRHAVGELTGAARAVLDAAGLAIDDVDWLVPHQANKRIIDAVGKKLGIAEAKVVVTVA
                     EHANTSAASIPLALDRLYTSGRLDRGDLLLCNAMGGGFAWGAALIRW"
     gene            complement(12107..13177)
                     /gene="plsX"
                     /locus_tag="P5558_01770"
     CDS             complement(12107..13177)
                     /gene="plsX"
                     /locus_tag="P5558_01770"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRY23068.1"
                     /translation="MPMSVVIALDVMGGDKAPEIVIEGADLALGRNPELRFLLFGDEA
                     RIGPLLAGRPALRQAAELHHTPDAVAADAKPSAALRQGRNSSMRLAIDAVQAGRAGAA
                     VSAGNTGALMAMAMIVLKTLPGIDRPAIASVLPTKRTPVVFLDLGANSVCSAEMLFQF
                     AVMGEVYARAVLGVAKPRVGLLNIGTEEVKGDGVVRSAAALVRDSGLPIDYVGFVEGT
                     GVTAGTVDVVVTDGFTGNVALKIAEGTASMFTHSLRQAFASSLRAKLAYLLARPALHG
                     LRDRFDPRLYNGAMFLGVNGVVVKSHGGTDAVGFAAAIRAALDLISRGTNERIIEEMG
                     RVAIPDADEDDGDASEPQAATG"
     gene            complement(13248..13802)
                     /locus_tag="P5558_01775"
     CDS             complement(13248..13802)
                     /locus_tag="P5558_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRY23069.1"
                     /translation="MAQDPEFSRLMMLGELPERDGRPFAIAADPEECRRVAERLELVA
                     LGRLAFGGRLEHGPRAGVLILRGRLEADATQRCVVTLEPVVAHLDVEIERYFVLGPEV
                     EVDEILVSPDDEEPEPLDGTCLDLGEIAVEELALALDPYPRAADADAQLEAQRAAIQG
                     GAGTDAARSAFAALAALRDQGKGT"
     gene            complement(14015..14653)
                     /locus_tag="P5558_01780"
     CDS             complement(14015..14653)
                     /locus_tag="P5558_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HRY23070.1"
                     /translation="MTSREQETWPVSRGLFGRFSGWLGSRRADRRRLAKAFALYHGIV
                     ARAREPRFYAEWGVPDTPDGRLELIFAHTSLMLRRLRAEGEAGRTLAQDLFDVLFADV
                     DRNIREQGVGDLSVGKHVKRSAQTFLARAQALDAGLDAGDLDGIARTLDRNIHNRVLP
                     RAAEGGPVEGAPETTPPAGMLRLATHLRDEAARLERLDGAALLAGRCEPAGG"
     gene            14754..15221
                     /gene="bamE"
                     /locus_tag="P5558_01785"
     CDS             14754..15221
                     /gene="bamE"
                     /locus_tag="P5558_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRY23071.1"
                     /translation="MPSKPSATHLVLALGLAGGLAACSPQVSQHGYTVDEAAVQRIVP
                     GITPREEVARLLGSPSTLATFEDNRWYYVTQKLERLSFYQSEITEQTVLTIAFDDRGI
                     VQDVSHVTMDEAMEIDPNANVTPTLGNELTVFEQLIGNVGRFGDPVAGKIGGK"
     gene            complement(15297..17414)
                     /locus_tag="P5558_01790"
     CDS             complement(15297..17414)
                     /locus_tag="P5558_01790"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRY23072.1"
                     /translation="MSGFEWFLLILIIGCGAAGVAYGILTRQIILALPTGTPRMQEIA
                     AAVQEGARAYLNRQYRTIAMVGVVVFILLTVFLGFSVGIGYLIGAVLSGLTGYIGMNV
                     SVQANVRTAQAASGPNGLAAGLDVAFKAGAVTGMLVVALGLLAVAVYYGILMIIGVET
                     RTILEALVGLSFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIA
                     DNVGDNVGDCAGMAADLFETYAVTVVATMLLGAIFFSGPATATLMIYPLAVGGICLLA
                     SIAGTFLVKLGPKKNIMGALYKGFWGAAGISLVLLLPLTALVLGFGSTGYEAKVGDVV
                     VKTFGGRQIYYCTLVGLAVTGALMWITEYYTGTNYRPVKSIAKASTTGHGTNVIQGLA
                     VSMESTAMPVIVICAGILVAYLNAGLYGIAVAATTMLALTGLVVSLDAYGPVTDNAGG
                     IAEMADLPANVRETTDALDAVGNTTKAVTKGYAIGSAALAALVLFAAYVQDLKHYFPD
                     LDVTFVLSDPYVVVGLFLGGMLPFLFGSMGMQAVGRAAGAIVEEVRRQFRENSGIMAG
                     TSKPDYGRAVDLLTKAAIREMIIPSLLPVLAPIVVYFVISWVGGQAAAFTTLGAMLLG
                     TIVTGIFVAISMTSGGGAWDNAKKYIEEGNYGGKGSDAHKAAVTGDTVGDPYKDTAGP
                     AINPMIKIINIVALLIIAVLAAH"
     gene            17720..18343
                     /locus_tag="P5558_01795"
     CDS             17720..18343
                     /locus_tag="P5558_01795"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRY23073.1"
                     /translation="MQLGLSLSRGSLPLAAFLLAAGPVAAEEVFTFDPGHTEIQFAYS
                     HLGNSRAFGAFEGFTGELALDTDDPARSRIEVVIDAASLRTGVDALDGHLKSADFFAV
                     ESSPEIRFVSTGVVPTSETTAEVTGDLTIRETTVPVVLDVTLNYLGDHQLGQFVPDYA
                     DMEVAGFSARGTVLRSAFGLDMLVPMISDEVELVIEAELLRPKVLVD"
     gene            18646..19197
                     /locus_tag="P5558_01800"
     CDS             18646..19197
                     /locus_tag="P5558_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRY23074.1"
                     /translation="MRLTNSPDGYGFVHWALHWITAALIFWLFWLGLTMVELPISPQM
                     FQDYALHKSLGLTVLVLVLARLAWRRLTGVPALPDGMGRLESLVATAVHHGIYVLLLV
                     IPLSGWLYSGATASPANWFGFFTVPDLIGANEAVAKVLRAVHDIAGKLLMLCLALHVL
                     GALKHHFISRDAVLKRMLLPRRS"
     gene            19202..19753
                     /locus_tag="P5558_01805"
     CDS             19202..19753
                     /locus_tag="P5558_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRY23075.1"
                     /translation="MRLPVALLVLLTALPAAARDWRMIDDESSLAFTFKQMGSPVTGR
                     FETFTAAISFDPAAPDAASVEAVIEIASVDTGNAERDAGIGGPDWFDAATYPTARFVS
                     TGFTATGGDSYDVAGELTIRDVTDAVVLPMTIAVDGDRAVATGTLELDRTTFGVGQGD
                     WASATAVGHEVLLEIEVVATAED"
     gene            19896..20114
                     /locus_tag="P5558_01810"
     CDS             19896..20114
                     /locus_tag="P5558_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRY23076.1"
                     /translation="MKSVQFVSIGHRLGMGMGKRPAGLADLVAWLGRSMRRSRGRSSL
                     TELDDRLLRDIGISRAEARRESSKPFWR"
     gene            complement(20137..21537)
                     /gene="hemN"
                     /locus_tag="P5558_01815"
     CDS             complement(20137..21537)
                     /gene="hemN"
                     /locus_tag="P5558_01815"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRY23077.1"
                     /translation="MAAPTETSVQPGEAAPALPVEQLGRLFEADRVPRYTSYPTAPHF
                     RPDIGSVAAAEWLAALPPDQPASLYLHVPFCRQLCWYCGCTTHITRHAGRINAYAQTL
                     ARELDLVADHLREPVPLAHLHWGGGTPSSLGTADLATLMRRIDERFGFTADAERAIEL
                     DPRFVSTELADCIARIGINRASFGIQSFDPAVQRAINRIQSVDTTRLALERLRSAGID
                     AISFDILYGLPHQDVASVLDTVDSVAALAPGRVAVFGYAHLPGLKKHQSMIPDELLPD
                     AAQRLAQFAAITERLEALGYVAIGLDHFARPDDPMARALEAGRLERNFQGYTTDRATT
                     LIGVGASAIGSFKDGYLQNHVDLQAWRSAIAEGRLPIARGLRLSADDRHRRAIINRLM
                     CEGRADLGAIADAHGAAAIAPEPGRFARLVELGIARREDDVILVPARYRPLLRLVAAC
                     FDRYLAPGQGRHSLAL"
     gene            complement(21673..22728)
                     /locus_tag="P5558_01820"
     CDS             complement(21673..22728)
                     /locus_tag="P5558_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23078.1"
                     /translation="MTQDQTRVDDALDNDRLLRFVDGALDDDQRVQVLTALSSRPETV
                     ARVEAYLHQNARLRELGQHLPMHDSRTFRADLQGEIVRRLAGRRTRVAAFRYAAAAAV
                     ALTVLGGGTALTLDRLAGSGTSALRSSAGAPEIYFPFGTTQLAASEPAGLVTQTDAAP
                     TDLASVTWLAGQVPGLTLSAPELDGIGLHLVRGETLDANRAPAIRLIYADEVGSRVVL
                     YAAVARSNADHAFRLVPEGHLSLQWRRGPMIFALVAPTDSPQLSAIVELVGKGVAQAL
                     PRETAEPVPGTVPAAAPADGGPIQSIAVPPARPVPEVPKAAPEPAAATTATTTGDGVI
                     TPPTLPVATKANEPKPL"
     gene            complement(22725..23273)
                     /locus_tag="P5558_01825"
     CDS             complement(22725..23273)
                     /locus_tag="P5558_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY23079.1"
                     /translation="MIWQRGKMRSEAASQAIAEELVVHVAALRRYALLLLGDSADADD
                     AVQETLTRVLARAKSWHTIGDLRPYLFSTLHNVFVDSTRREKRMATDFLSEDILASLV
                     TPPSQQQHVELRDTVVALAKLPNEQREVVLLVGLEGLSYLEAAEALGIPVGTVMSRLS
                     RGREVLRSMTSRETSRKLRVVK"
     gene            complement(23568..25352)
                     /gene="phaC"
                     /locus_tag="P5558_01830"
     CDS             complement(23568..25352)
                     /gene="phaC"
                     /locus_tag="P5558_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRY23080.1"
                     /translation="MAGDKELPDARELAARMAEIGERSNRIFRRFLERQDDEHFQIPD
                     PKVVTDAFARLAEHLLARPERLVDAQLAYWREMGRLWESYMRRVLGEEVPPMVEPAPD
                     DRRFRDDAWSEDLVFDYIKQSYLVTASWIQGTVANVEGLDPALRSKVAFYTRQYVDAL
                     APSNFALTNPVVLRQAAETRGESLYEGLRRLLDDLERGKGELKISMTREDAFELGRNI
                     ATSEGAVVFENELMQLIQYAPLTPTVQRRPLLIVPPWINKFYILDLKPKNSFIRYAVE
                     QGSTVFVISWVNPTAELAHKSFEDYLVDGPLAALDAVTRQTGEDEVNIIGYCLGGTLA
                     ACLLAHEAAKGQKRIASATFFTTMLDFAEPGDLAVFIDDEQLELLDRHMAKKGYLEAR
                     HMQQVFNLMRANDLIWSFVVSNYLMGRAPLAFDLLYWNADSTAMPYMMHSFYLRCMYQ
                     QNLLTRPGGIILDGVPIDLGQVRQPAFFVSTREDHIAPWRSTFRGSRLLGGPVRFVLG
                     GSGHIAGIVNPADSTKYGYWTSTSRSADPDTWLARAEHHEGSWWPAWRQWLERHGGGT
                     SPARDPGAGNLEVIEPAPGRYVRERSPD"
     gene            25458..26165
                     /locus_tag="P5558_01835"
     CDS             25458..26165
                     /locus_tag="P5558_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HRY23081.1"
                     /translation="MDKSVATKLAGVSTATLTTCLFKRGLRNQFLQDVHPVGGSGTRM
                     VGPAFTLRYIPAREDIDVLDAYADFEHPQRKAIETCPAGHVLVMDSRKDARAASAGDI
                     LVSRLVMRGVAGVVTDGGFRDTPSIAALAIPAYHQRPSAPTGPILHHAADIGLPIGCG
                     DVAVYPGDIMVGDGEGVVCLPAHLAAEIADEAFEMTVYEDFVESEVKAGRSIFGTYPA
                     TDASRARFAEWRKAQGR"
     gene            complement(26219..26821)
                     /locus_tag="P5558_01840"
     CDS             complement(26219..26821)
                     /locus_tag="P5558_01840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY23082.1"
                     /translation="MHGADLFLIRHGETSWNLERRFQGHRDSPLTGRGIEQGERAGRR
                     LLAELDEPAAYGLTSSPLGRARHTAAIVARAIGRTPREITLDDRLKEMTWGAWDGLTA
                     AEIEARDPELWQARINDRWTVAPPGGGETQQAILDRAMAWLEDLAPAARLVAVAHGAL
                     GRAIRCAYLNLPAAAMLELDEPQDAIFRLCCGRIERIEVL"
     gene            complement(26828..27211)
                     /locus_tag="P5558_01845"
     CDS             complement(26828..27211)
                     /locus_tag="P5558_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23083.1"
                     /translation="MSCGLLALVVLGGGSLPADAADGLFGSPSARTSPSLERQIDRAR
                     RDPAGTSTLQRRNLQDQLRQEPPGAERTRQLNTLDRLPPPRPATGPEPVPAPLPTGQL
                     PTSIPAPAGGLPTSLPPLVPRPAPR"
     gene            27374..28678
                     /locus_tag="P5558_01850"
     CDS             27374..28678
                     /locus_tag="P5558_01850"
                     /inference="COORDINATES: protein motif:HMM:NF021633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HRY23084.1"
                     /translation="MSSKVAHSQGPGKSRRDPRLDFFRGIAMLIILVAHIQWNSWSLW
                     IPARFGPSDAAEMFVFCSGYAAAIAFGGTFVTAGFWMGVRRIALRCWQVYWAHLGLFF
                     TVAAITVVANELFGTPRWNYLGVLNLEPFFDDTQTQLVGLFTLTYVPNYFDILPMYLV
                     ILAMIPLVMLLHRLHLAAAIAFCILLYVATWVFNLNLPAEPFSDRVWFFNPFGWQIVF
                     FTGFMLSRGWVRPPPYRPWLLALAAGYVLVMIPISRWQIYMQVPALEAVHDWLWLKDW
                     EQGGIFKTNQHPLRWLHLLALAYLALWVLRGREHWLDTAPCRVIIKVGQQALATFMTS
                     MALAWILSIALDQLGGEGTTFAGKYRPGREPLSVAAANIFGLAAIIGVAYMVDWYKRE
                     PWRRVGQAPAQSVSGSRAAAQPAAAAPESAALAVAPGARAYR"
     gene            28980..29780
                     /locus_tag="P5558_01855"
     CDS             28980..29780
                     /locus_tag="P5558_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23085.1"
                     /translation="MSPGLTRRTLLLAGALLPLRVAAAAELPVPPCAGETPRPAPPRL
                     GQPPAVLACTDSDLLAAWQPPACLGWRAKPADVVLALAARFRLDDIEALLARFTAISA
                     LTRIRYWSVTREAWRPLFLDAVPLSRADPAATRGDFRRDEIVLGEPVHVLLEDDLIGA
                     VVHSITVEAFSPELIRVAMRNVLPGGVLGMEVLPVDGLEMVLLLEREEPSIWRYWSIM
                     RATIEAPDFMLPSAASYANRAAAIFRYVASLPTDAEPPLVRRSRDLPS"
     gene            29843..30718
                     /locus_tag="P5558_01860"
     CDS             29843..30718
                     /locus_tag="P5558_01860"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY23086.1"
                     /translation="MVDCVLDAKAQVGEGAMWDETLGRLWWVDINAPTLNRFDPATGE
                     NRAWTMPSRIGCFGFRAKGGFVVALEDGFSTFDPETGAIEAIVDPEPEWPENRFNDGT
                     PDSAGRLIAGTMPMGERLPVASIWRLDADRRVSRLVDGLRVSNGMAFSPDGRLFYWSD
                     SDPSVQTIWVADYDPASGTLGRRRVLAKTDELAGRPDGGTIDADGCYWMAGVGGWQLV
                     RFTPDGRVDRIVDMPVERPTKIAFGGADLKTLYVTSISGGQTPGTEARQPQAGGIFAL
                     SVPGVQGLPAWRLAV"
     gene            30700..31269
                     /locus_tag="P5558_01865"
     CDS             30700..31269
                     /locus_tag="P5558_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HRY23087.1"
                     /translation="MAPGRLGRPAKRPTPERLRRRALAYLERFSTSRAHLGTVLRRRA
                     LREAETLELEAAPVEAAIEALLDDLVRLGLVDDRAFAETRARRLVEKGRPARRIVQEL
                     AAKGVDRNVAMGVLEGLGEETPDLDLAAALAFARRRRLGPWAVPGGRERTPEQALAAF
                     ARAGFPYAVARQVLEAASLDELEAEVRDA"
     gene            31844..32116
                     /locus_tag="P5558_01870"
     CDS             31844..32116
                     /locus_tag="P5558_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23088.1"
                     /translation="MPLEELVGPGGAFPTYMLAVFLLPLAYGAWRFVLFHIAAGPLLA
                     STLTDNPNEMPAIWCLFSVGILLIALSPAIRSRFGQAGSWARPAAG"
     gene            complement(32141..32740)
                     /locus_tag="P5558_01875"
     CDS             complement(32141..32740)
                     /locus_tag="P5558_01875"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRY23089.1"
                     /translation="MPLQVIGTGFGRTGTDSMRTALNMLGQGPTHHMLELSDRPELYR
                     LWIELARGAEPDWDALFDGYRACMDWPSAYFWRELIELNPQAKVILTWRTAESWWASF
                     EKTILKVWRWPIEKSPLIHWVGENVFGGRPDDRENAIAVYERHVEEVIATVPADRLLV
                     HRLGDGWEPLCAHLGVPVPAEPYPSRNTTAELQARMSLE"
     gene            complement(32965..33390)
                     /locus_tag="P5558_01880"
     CDS             complement(32965..33390)
                     /locus_tag="P5558_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY23090.1"
                     /translation="MTLLDPDRIAIVKPGGTYVGKQGFTYGAGASAETVGATRICMNV
                     LPMPPGARARAHYHEGIETIAYLLEGRCTVRFGQRLEHRVELSAGEQGFVPADLPHAP
                     CNDSGAPCVWLVVHSSGSDQDGIVMLPELDEVLARAASA"
     gene            33389..33958
                     /locus_tag="P5558_01885"
     CDS             33389..33958
                     /locus_tag="P5558_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23091.1"
                     /translation="MANAPHGSRGGGAMARVGLPAALGQAVSAKRGVLETENSLGYEG
                     GAPLFGEKVKSEVTSFSGGLMCKMPSSINVFKDAYLNAIRDVIFKNRLSNFLDEFPWL
                     TGHLGNPFAPVWFIGENPSRSRVEHIDKVSTSHSENLQWSYKFREARLFRDALTKAGL
                     KEGDPAEDIGWNCYITNVTKEPEVVKKKK"
     gene            complement(34287..34658)
                     /locus_tag="P5558_01890"
     CDS             complement(34287..34658)
                     /locus_tag="P5558_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY23092.1"
                     /translation="MSTITTVRARINGDRVYLIAADGSETPLVPEIDRARLDAADAAE
                     IARQIAADDAEARAAAAAHIRGLRARTGLSQAAFARRLDVSAETLRNWEQGKRLPQGP
                     ARALLRIIDKAPDTAMAALGG"
BASE COUNT         5179 a        11951 c        12437 g         5286 t
ORIGIN      
        1 ggagctcgag gcggagcgcc gtgcccagtt cctgtcgcgc cacgatgccc tgaccggcct
       61 gcccaaccgc aacatgctgg ccgagcggct cgacgagacg atcgcccagg tcgcccggcg
      121 acgcgagatg gccgccgtgc tctgcgtcga tctcgaccgc ttcaaggacg tcaacgacac
      181 gctcggccac ggcgccggcg acgcgctgat tcgccaggtc gccgagcggc tcgaggcctg
      241 cctgcgcgag accgacacgc tggcccgggt cggcggcgac gagttcgtgg tcatccagac
      301 ggcggtgcag gacgtggccg cggtcgagct cctcgcccgc cgcatcctct cggtcgtcga
      361 gatgcccttc gacatcgggg tcgagagggt tctcgtcacc gccagcatcg gcatcgccat
      421 gctgcccggc gacggcgcga tcagcggccg cctgctgcag aatgccgata tcgccctgga
      481 tcgtgccaag agcgaggggc gcagcacctt ccgcttcttc gagcgcgaca tggatgcccg
      541 gctgcaggcg cgcaaggcgc tcgagcgcga tcttcgcctc gccatcgtgc gccaggagct
      601 cgagctcttc taccagccca agatcgacct gaaaaaaggg cgggtcagtg gcgtcgaggc
      661 gctggtccgc tggaaccatc cggaacgggg catggtctcg ccggccgact tcatcggcgt
      721 tgccgaggag accgggctga tcgtcgagct cggcgcctgg gtgctgcgcc gggcctgcac
      781 cgacattcgc gggcatcgcg atatcgagct cgcggtcaat ctctcgccgg tccagctcaa
      841 gcgcggcggc atcgccgaga tggtcgccga catcattgcc gaaaccggct tcgatcccga
      901 ccggctcgag ctcgagatca ccgagagcgt gatgatcgag ggggccgagg cggcgatcgc
      961 cgagctgcgc cacgtcaagg acatgggggc caggatcgcc atggacgatt tcggcacggg
     1021 ctattcgagc ctgagctatc tcaaccgctt ccccttcgac aagatcaaga tcgaccgctc
     1081 cttcgtcatg gatctcggca agcggggcaa tggtgccgcg atcgtcaagg cggtggtcgg
     1141 catcggcgag agcctcggca tgacgatctg cgccgagggt gtcgagacca tcgagcagcg
     1201 ccgtttcctg gagcaggccg gctgccacga ggcgcagggc tactacttcg ccaagcccat
     1261 gccgctggcc cagctgaccg gcttcctggc cgagccgacg accgtgctgg cgcctgcctg
     1321 aggccggttg cagcgaatcc cttttcgctc attgcgagaa attttgctgg tgactatact
     1381 tatgagttga gttcggctat cccggtcggc ggcggcgacg ccctggctcc ggcagtcccg
     1441 ccgggccggc gaaccaccgg gaaaccgtca tcatgagcga cgtgctgtca cggctcaaga
     1501 agctgaccaa gtgcctgcac atctaccttg ccgaaacgcc cggtcgcggc ttcggcatct
     1561 tcgccgcacg cgcctttgcc cagggcagca cgctcgtcgt cgacgaggac ggcgactact
     1621 atgccggcga gatgaccctc gccgaggccc aggcccgcgg ctacgatctc agccaggatc
     1681 tcttccagat cggcaccgac gcgttcctgc tgccgaatgc caatctcgac gatttcgtca
     1741 accacagctg cgatccgaac accggcctca ggctgacccc ggccggctac cggatgatcg
     1801 cgctgcgtgc catcgcccag ggtgacgagc tcacctacga ctactcgacc tacatctccg
     1861 gcacgcccga gcggatgagc tgcgcctgcg gggcggccaa ctgccggggc acgatcggcg
     1921 ccttcgagac cctgcccccg gccctgcagg accgttatct cgcagccggc gtggtcgggc
     1981 gcttcgtcgc cgccgctgcg cgcaatgccg ccatccagga cgctgcccgt gccgaaagcg
     2041 gggccggcgt ccgggccggt ggcgaggcgg tgtcggccta gccggcggcc aaccgggccg
     2101 gtaggcggag ccggccggct gatggccgca agcggcgggt tgcggggttc ggtcgtggtg
     2161 cggctggcgc tggtggcagg gctgctcagc gccggcaagg tcctgctcta caccgccggc
     2221 accacgctct tcctcgcccg ggacggcatt gccggcctgc cgctcctcta tgtgaccctg
     2281 gcggtgctcg ccacgctcgt ctcgatcggg ctcgggcaca tcgtcgaccg ggtgccgggc
     2341 ggccggctcc tgccgggcct ggcgctcgcg atcgccgtgg tggtggccgg cctgctgggc
     2401 ggcctcgtgc tcggcctcgc ctgggcgccc atggggctgc tgctcgccgc ccatctctac
     2461 gacatcgtca ccgacatcgt cttctgggtc ctggtcgccg ccttcctcga caacctgcgg
     2521 ctgcgccggc tgacgacgcg gctctatatc gccatcgcca tcggcggcag cacggccggc
     2581 atcctcgccg agcgggcact cgtctggctg tcgcccggcc agctcctcct gccggtgatc
     2641 ctgctcgccg gcctcgccgc cctcgctttg tggccagtgg cggtgccgcc gacgacgggc
     2701 gcgggcggcg aggccggcga ggtcgggacc gggctcggcg atctcgggcg gttcctcgcc
     2761 cgccatccct tcgcgctgct cctcgccctc aactcgatga tgctgaccgt cgtctacagc
     2821 ctcaccgagt tcgtctgctt cgccctctat gccgagacct atgcggacga ggccgagctc
     2881 ggccggttcc tcgccctgat cttcgccggc ctgcagctcg ccgagctcgc cgtgctctgg
     2941 ctcgcggcac ggggcgtggt cgagcgggcc ggccccttcc tgcgcaacct gctcttcccg
     3001 ctgggctcgc tcgcctgcct cgcggccctc ctggcccagc cgcgcctcgc ctgggcgatt
     3061 ctcgcccatg tcaataccga ggccgtctcg aacgccgtgt tcgagccggt caacaccacc
     3121 aattacggtg ctttgccggc ggccgtgcac ggccgggccc ggaccctcgc cgacggcgtc
     3181 ttctacccgg tcggcatggc gctggccggg ctcgtcctcg ccatcctgcc cgccgacgac
     3241 gccgcgttcc gggcccgcgt gctggcgctc gtcgcggccg gcttcttcgt cctgctcaat
     3301 ctcgtggtgg cgcagcgcta cttgccgacc ctggtccgcc acctgcggca cgggcagcgg
     3361 ccacgcctgc cggcagcgcc gcggcggcgc gcagcaagcg gcgatccggc gatcgacccg
     3421 gcccggctcc tcccgctcct cgccagccgc cgggcagcgg accggctgct cgccgtcgat
     3481 ctcgtcgagg ccgccctggc acgtgcccag ccggaagctg ccgaagcgct ggtcgcggcg
     3541 ctggccacgg cgctggtcgg ggtggcaccc cggctcggcg agaccgactg ggcccgggtc
     3601 gtggcgatcc tggcgcgggc cggacccgac gccctggttc cagcactcga agcggcgacc
     3661 gccaggaccg aacgcgaggc catgtcactc ctcggcgccg cgtgcctgct cgccggcggg
     3721 gcgcccaggg tgccggtggc cggcgatgtc gccctggcgc ttgccgatct cctcggcggc
     3781 tgtccctgga tgcaccccaa cagcacggcc gaccagggta gcccgggctg gcgcaacgac
     3841 gcccagctca tcggccgtct ggctgccgtg gtgcggcggg caccacctgc cttgacacag
     3901 cacctggccc aggggatcga ccacgccgaa acgcccgaag agctgcgcca ggcgctgctc
     3961 gaagccgtgc tgcgccatcc gcccaaaacc cccgatccgg cgctgctggt gctcgccgag
     4021 gaggggcggc acgccgcggc gccggcgcat cgggcgatgg cgctcggcgc gctcgcggcg
     4081 atgctgccgc ccgccgagct gctcggtccg ggcatggcgg cgctgaccga tccgagccgc
     4141 caggtgcgac gggtcgccgt cgatgccctc ctgccgctcg ccgaccgcgc actggcggtg
     4201 ctgctggtgg acggcggcag ccgcggggcc gcctccctcg gggtcatcga gctcctcgcg
     4261 gcggtcgata gccggccggc acgtcgtctg ctcaggcgcc tgatcgccgg cctcgccgag
     4321 ctcgccgagc gcgacgcgcg cctgctcgac cggatcgcgg cggccggcga cacgtcgctg
     4381 gccccgctgc atgccgccct gctcgatcac gcgcgaagcc tcacggaccg gctcttcgcc
     4441 gggctcgggg ccctcggcga ggcgggccat gcgatggagc ttcgccgcgc cctcggcgag
     4501 gccgacaccc ggctcgtcgc cggcggcatc gacggcctcg tctcgctccg ccacggccag
     4561 ctcgcccggc gcttcgtgcc gctgctcgag cggctgcatc tgccgcacca aggggcggcc
     4621 gccgaccagc ttcatgtccg ggccggcgca tcggccgaca ccgccatcga gcgactggtc
     4681 gaggacatcg acggccgctg gctgcgagcc gcgacccggc tcctgcggcg acgccgggcc
     4741 gacgctccgg ccgcacccga cctgcccaca ccgaccgacg aggtggccat gccgatcttc
     4801 gcccctcccg cccgctcgac ccacgacgac cggctgttcg aggcgatgct ccgcctcaag
     4861 cgcttcgacc tcctggccga gctgccgctc gatgcgctgg aggtgctggc gcccctggtc
     4921 gaggaacgcc gcttcgccgc cggcatgacc atccagcgtg gtggcggggt gctgccgctc
     4981 gcctggctgg tcgacgaagg cagtgtcgag gtgagctggc cggacggacg gatcgagacc
     5041 gagggcccgg gcgggatcat cggcgagacg gcgctggtcg acccgaccgt gacggcgccg
     5101 ggggtcaccg ccggcaccgc ctgccgcctg ctgcagctgc atcggatcac cttcgaggac
     5161 atcgcccggg accatccggc gctgaccgaa gcactctgcc gcctgctcgc gcggcgtctt
     5221 cgcgaggcgc gcttgcaccg cggccgccag cccgacacgc ccacgacgca aatggcgcga
     5281 cagcccgaga gcaggcggga gaccgcgtcg gcggttggcg atcgatgaac gcacccggcc
     5341 gcgccgcgcg gcgcggtcgg caggaagccc attcagaaaa gcgcttgtat cgcgcgggcg
     5401 gtgccggcaa cctcggcgcc agggcggacc gggaagaccc gccgacccca ataacagccc
     5461 tgcaagcaag cctgaaagca gaaatgagcg acgatgacgc gccgagcctg gccgagctga
     5521 cgacccggat cgtcacggcc tattttcgca gccacgacct cgccgccgcc gatgtcggcc
     5581 gcctggtcac gacggtgggc acggagctgg gcagcctcgg ccgcccgccc gagcagccgg
     5641 ccgcggcgga gccggccgtg ccgatcgagg actccgtgcg acgcgacggc ctcgtctgcc
     5701 tcgtctgcgg cagacgcatg aagacgctgc gccgccatct cctctccgcc catggcctga
     5761 ccgccggcga ctaccgcgag agatacgcgc tcgaccgcga ctatccgatg gtcgccgccg
     5821 cctcgtccgc gcggcgggcc gagatcgcgc ggcggaccgg gctcgggctg cgcacggcgg
     5881 tcgagcctgt cccggcgccg gcggtgccgg tgccgcaagc gcccgagccc gcaccggagc
     5941 agccgaagcg ccgactgggc gtgaagccga tccgcggccg gcgggcggcg aagccggccg
     6001 gcacagaaga tccggcgcgc cagccgcagc ccgagccggc gcccgagccg aagcggcctc
     6061 cgggcgcggg gggatcctcg acctagacgt cgtcgaagga ggcgtagttg aggcggtaga
     6121 ggcgggcgta gagcccgttc tcctgcatca gctcggcgtg gctgccggtc tcgatcacct
     6181 ggccgtcccg cagcacgacg atccggtcgg catggcggat ggtggcgagg cggtgggcga
     6241 tgaccaacgc cgtgcggccc tcgagcaggc ggaccagcgc ctgctggatc aggcgctcgg
     6301 tgtagctgtc gacattggcc gtcgcctcgt cgaggatcag gatgctggca tcggcgacga
     6361 gggcgcgcgc gaagctgatc agctgccgct ggccgaccga gagattgccg ccctgctgct
     6421 cgagcggcgt gtcgtagccc tccggcaggg ccatgatgaa gtcgtgcgca cctaccgcgc
     6481 gcgccgccgc ctcgaccgcg tcacgggtcg cccccggcgt cgcgtaggcg atgttctcga
     6541 gcacggtgcc ggtgaagagg aaaggctcct gcaggaccat ggcaatatgg cggccgagcg
     6601 aggcctgggt gaggtcgcgc acgtcgtggc cgccgaccag cacgcggccg ccgccgacat
     6661 cgtagaagcg atgggccagc gccatcgtgc tgctcttgcc ggagcccgtc ggcccgacca
     6721 gcgccaccgt ctcgccgggt cttgcctcga agctcacgtt ccgcaggacc gggacgccct
     6781 tcacatagcc gaaggtgaca tcctcgaaga cgaccgagcc gtcgatcgtc tccggatcga
     6841 gcgcatccgg ccggtcgacc acggcgatcg gcacgtcgag gacctcgaag atccgctggc
     6901 ccgaggccat ggcgcgctgc atgacgctgt actgcatggt gagcgagcgg atggggtcga
     6961 agaagcgctg cacgtagaag aggaaggcga ccatcacgcc gaggtcgagc gcgccgtcga
     7021 gcacggccat gccgccgaag acgacgacga tccccatggc cccgccggtc agcgtgtcga
     7081 cgatcggcac catgagctgg gcgagccgcg tgccgtgcag ctgggcgtcg agattgtcgg
     7141 ccgccttctc ctcgaagagc cggagattga ccgcctcccg gcccatgccc tggaccgtgc
     7201 gcacgccatg gatgccctcg gcgagagcac cgttcagggt cgagctggtc acccgggcgc
     7261 gcaggaaggc gcggcgcgcc cggggcagcc agatgagccg gacgatgacg aggacgggca
     7321 tgaccgagag ggtcaggagg ccgagccgcc agtcgagcac caggaggacg accgtgatcc
     7381 cgaccaggag aacgaggtcg ccgatggcga agacggacgt ctcgaggaac tcctgcaagg
     7441 catggacatc gccctgcagc cgggacatca gccgaccgac ctcggtcttg tccatgaagg
     7501 acagggagac gcgctgcaga tgggcgaaca tggcccggcg cagatcgacg agcagccgct
     7561 cggcgatgcg cgccaccagc gtctcctgga cgagattggc gacgaaattg agcgagacga
     7621 cggcgaagaa ggcggcgacg ccgacgagca gcaggcgctc ggccgccatg gcggcgtcgg
     7681 cggtctcgcc ggcgagggcg gaatcgacca cggcgcgcag gatgagcggg atggcgacct
     7741 ggctcagcgc gaaggcgagg acggcggcca gcgccagcag gagccgtcgg ctgtaggggc
     7801 tgacgaactc ccagaagcgc ctgaggatcc ggccgtcgaa ggcggcgaag atctcctcct
     7861 cgttgtcgat ctgcgagccg accacggcat gcggcggccg cgccgcctgc gccggtgccg
     7921 ccggtcgccc ggcgacgtgc ggcaccctca tcgctccggc tccgcgaccg gtgacggggc
     7981 cgccggagcg cccgccaccg gctcgttgcc caggctctgg agcgcgtaga ggctggcata
     8041 gcggccgccc agggcgagca gctcctggtg cgagccgcgc tcgacgatgc ggccgccttc
     8101 gaggaagacg atctcgtcgg catccatgag cgagcccagt cgatgggcga tgatgatcgt
     8161 ggcgcgcctg gtgctctggc tgcgcagggc ctcgcggatg ccctgctcgg tcgcggcgtc
     8221 gacggcggcg gtgctgtcgt cgaagatcag gatcggggcg tccacgagga tggagcgggc
     8281 gatcgagagg cgctggcgct ggccgcccga gagcgacacg ccgcgctcgc cgaccagggt
     8341 ctcgtagtcc ttcggcagcc cggcgatata gtcgtgcaac cgcgccatct cgctcgaatg
     8401 ggcgatgcgg tcgcgctcgg cccagggatt gccataggcg acgttgttct cgatcgacgc
     8461 cgtgaagagg aaggtgtcct gctggaccag gctcacggcc cggcgcagcg agcccagcgt
     8521 cacgtcgcgc acgtcctggc cgtcgatggt gatccggccg ccgccgacat cgtagtagcg
     8581 cgggatcagg tgcgcgatcg tcgacttgcc gctgccgggc gggccaacga tgccgagcgt
     8641 gcgaccggcg cggacctcga agctgatgtc gtgcagggtc gcgagcgtgg ggcccccgcc
     8701 gctggcccgc gcgccggtcc gcgcgccggc ataggtgaag gacacgttct cgaagcggag
     8761 cgccgtgtcg gtgatccgca gctctttcgc atccggggca tcctggatct cgggctcgcg
     8821 atccagcacc tcgaagagcc ggccgccgga catggaagcg cgggcgaagg cgttgaccat
     8881 catgccgagc tgccggaccg gcatctgcag gatggccatg aaggccagga actcggccag
     8941 cgtgccgacg ctgatctcgc ccgccagcac cttgccgccg ccgacccaga gcaggagccc
     9001 catggcgagg aagaaggcgt aggtcatggc cgccgtgttg cgcacccgga tgtcgatccg
     9061 gtgatcggcg agcgccagcg tctcctccga gatggcgtcg aacttgccga gctcgtgcgg
     9121 ctgggcggcg aaggcgcgca ccacgcgaat gccctggagg ttctcctcca tgacccgggt
     9181 gaggatgccg agcttctcct gcagcgccag ccagccggcg cgcagccgca ggcgcgccac
     9241 gaccgagcgc caggcggcga acggcacgaa gctgagcgcg atcagcccga gccagagatc
     9301 ctgcctgagg agcagcgtgg caccgacgcc gacgagcacg gtgatcagca cgagacgcag
     9361 gatgccggca ttgatcacca tgcggatgcc ctcgatgtcg agcatgccgc gggtgatcag
     9421 ctcgccgctg tgggtgcggt cgtggaagtc gaagctcagg cgctgcagct gggcgtagta
     9481 ggccatgcgc aggtcgtagg cgatgcgctg gccgatcgcc tcgccctggt agttgtgcca
     9541 catggtgaag aggccgcgca gcacgctggc gccgagcagc aggaaggcgg tccgccagag
     9601 cgcggcgcgc gccgcctcgg catcgccgcc ggcgagcagg ccgcgcgccg aatcgaccgc
     9661 cttaccgaga tattgcggaa tcagcagctg gaagacggcc gcgaggaggg tggtgacgag
     9721 ggcgatcgcc atgccgcgcc ggtagcggag cgccatcccc agcacccggc gcaggatgcc
     9781 gatatcggac aggccgaccg tgaccctggc cggcggggag ctgcgggtga tgaggggatc
     9841 gctggcgggc acgatggagc cgaatgccgg aggtgacggg ccgggcaggc cacgctacgc
     9901 gccacgtaac catggtcgat catggttccg ggcaggtgtc gtgtccggcg ctgcggaaat
     9961 tccctcctgc atagcgccaa ccggtgccgc gaccaatgct cttcgacgcc tcgggggcgt
    10021 catggcgacc ggctgtcgat cgaggcgccg gcggcagtgg tcggagcgcc gagatttgaa
    10081 ctcgggaccc ccagtccccc agactgatgc gctaccaggc tgcgctacgc tccgacgggg
    10141 cggactatag ccaagggtcg gaaggcgggc aagctggggg cgtcctcagc tatcggcttc
    10201 ggcgagggcc tgggcgagct cttcgcggag cgatacgaga ctgtcgcgga ggtggtggag
    10261 gcgggcccgg tcgggtgctg ccgtggccga tgcggcgggc ggcggcgccg cgttgctcgt
    10321 gggttcggcc gcggcggggt cgggcacggc gtcggtcgcg gccaggggcg gcgccggggc
    10381 cggcgggctc ggcttctcgg tcgtgcgggc gatcgccggc ggccgggcgc gacgctggcg
    10441 gagcagcttc tgcgccccct tgatggtgta gccctcgtcg tagaggaggg cgcggatctc
    10501 ccgcagcagg tcgatgtcct ctgggcggta gtaccgtcgt ccgccgccgc gcttgagcgg
    10561 ccgcacctgc gggaacttgc tctcccagaa gcgcagcacg tgctgggcga cgtcgagctc
    10621 gtcggcgacc tcgctgatcg tccggaacgc ggcctgggac ttgccgcgcg tctgcgcggg
    10681 gatcgccgcc tggtcggccg tcaccaccat ctcaggccgc cgggcgttct gagttgatgc
    10741 ggctcttcaa cacctgggag gcgcggaaca cgagcacgcg gcggggcagg atcggcacct
    10801 cctcgccggt cttggggttg cggccgaccc gctcgccctt ctcgcgcacg gcgaaggtgc
    10861 cgaacgacga gatcttgacc gtgccattca tcaccagcga atgcgcgatc tcctcgagga
    10921 tcgcatccac caggccggcg gattcgctgc gcgacagccc gacctcctga tagaccgcct
    10981 cggcgagctg ggcgcgagtc accgttctgc ccgtcatcgc ccgcgttccc ctcgcgtgtg
    11041 ccttcgatgg ataaaacgct agatgataca aaggtttccg tcaaccgggc cgacacgccg
    11101 cggccgcccc gcggggaagg ggcctaccag cggatcaggg cggcacccca ggcgaagccg
    11161 ccgcccatgg cgttgcagag caggaggtcg ccgcggtcga gtcggccaga ggtgtagagc
    11221 cggtcgaggg ccaggggaat cgaggcggcc gaggtgttgg cgtgctcggc cacggtgacc
    11281 accaccttcg cctcggcaat gccgagcttc ttgccgaccg cgtcgatgat gcgcttgttc
    11341 gcctgatgcg ggaccagcca gtcgacgtcg tcgatggcga gcccggcagc gtcgagcacg
    11401 gcgcgcgcgg cacccgtcag ctcgccgacc gcatggcgga agacctcgcg gccgttcatc
    11461 ctgagatggc cgaccgtgcc ggtcgaggag gggccgccat cgacctgcag atgggcgtag
    11521 tggcggccgt cggcggccag catggtggcg aggatgccct ggtcgagcgc cgtgccgggc
    11581 tgctccaggg cggtcagcac gaccgcaccg gcgccgtcgc cgaagagcac ggcggtgccg
    11641 cggtcggtcc agtcgagcag gcgcgagaag gtctccgccc cgatcaccag ggccgtgctg
    11701 gcctggccgg ccttgatgaa gttgtcggcg gtggcgagcg cgtaggcgaa gccggcgcag
    11761 accgcctgga ggtcgaaggc gatgccgggg ccgcagccga gcttgcgctg caccgccgtc
    11821 gcggtcgccg gaaaggtgtg gtcgggagtc gaggtggcga cgatgatcag gtcgacccgg
    11881 tcgggcccga gatcggcgcg ggcgagggcg cggcgggccg ccgccacggc gaggtccgag
    11941 gtcagctcgt ccggtgccgc gatgtgccgg cgacggatgc cggtgcgctc ctggatccag
    12001 tcgtcggagg tgtcgagggt tcgcgccagg tcgtcattgg tgacgatgcg gtcggggaga
    12061 tagccgccga cggcgcgcag gatcgatcgc cgcatgaagc cttcgcctag ccagtcgccg
    12121 cttgcggctc ggatgcatcg ccgtcgtctt cgtccgcgtc ggggatggcg accctgccca
    12181 tctcctcgat gatccgctcg ttggtgccgc ggctgatgag gtcgagggcg gcgcggatgg
    12241 cggcagcgaa gcccacggca tccgtgccgc cgtggctctt caccacgacg ccgttgacgc
    12301 cgaggaacat cgcgccgttg tagagacggg gatcgaaacg gtcgcgcagg ccgtgcagcg
    12361 ccggccgggc gagcagatag gcgagcttgg cgcgcaggct gctggcgaac gcctggcgca
    12421 gcgagtgggt gaacatcgag gccgtgcctt cggcgatctt gagggccaca ttgccggtga
    12481 agccatcggt caccaccacg tcgaccgtgc cggcggtgac cccggtgccc tcgacgaagc
    12541 cgacatagtc gatgggcagc ccgctatcgc gcaccagggc cgcggcgctg cggacgacgc
    12601 cgtcgccctt gacctcctcg gtgccgatgt tgagcagccc gacacgcggc ttggcgacac
    12661 cgagcaccgc ccgggcatag acctcgccca tgacggcgaa ctggaagagc atctcggcgc
    12721 tgcagacgga attggcgccg agatcgagaa agacgaccgg ggtgcgcttg gtcggcagga
    12781 cgctggcgat ggcgggccgg tcgatgccgg gcagggtctt gaggacgatc atcgccatgg
    12841 ccatcagggc accggtattg ccggccgaga ccgcggcccc ggcgcgaccg gcctgcaccg
    12901 cgtcgatggc gaggcgcatc gagctgttgc ggccctggcg cagggccgcc gacggcttgg
    12961 cgtcggcggc cacggcgtcg ggcgtgtggt gcagctcggc cgcctgccgg agtgccggac
    13021 gaccggcgag cagcgggccg atcctggcct cgtcgccaaa cagaaggaag cgcagctcgg
    13081 gattgcgccc gagggccaga tcggcacctt cgatgacgat ctcgggtgcc ttgtcgccac
    13141 ccatcacgtc gagcgcgatg acgacgctca tcggcacgac tcgtgactcg gcacggagcg
    13201 gcaccggcgc tcgcggccga cgatggcgca ggggccaggc acccgcctca ggttcccttg
    13261 ccctggtcgc gcaacgccgc cagggcggcg aaggccgaac gggcggcgtc ggtcccggca
    13321 cccccctgga tggcggcgcg ctgcgcctcg agctgcgcat cggcgtcggc ggcgcgcggg
    13381 taggggtcga gggccagcgc cagctcctcg acggcgatct cgccgaggtc gaggcaggtg
    13441 ccgtcgagcg gctccggctc ctcgtcatcg ggagagacca ggatctcgtc gacctcgacc
    13501 tccggcccga gaacgaaata gcgctcgatc tcgacgtcga gatgggccac caccggctcc
    13561 agcgtgacga cgcagcgctg cgtcgcatcc gcctcgagcc ggccgcgcag gatcagcacg
    13621 ccagcgcgcg ggccgtgctc gaggcggccg ccgaacgcga gcctgcccag cgccacgagc
    13681 tcgagccgct cggccacccg gcggcactcc tccgggtcgg cagcgatggc gaacggccgg
    13741 ccgtccctct cgggcagctc gccgagcatc atcaaacgcg agaattcagg gtcttgcgcc
    13801 aagacagtcc cctccccgcc agtatggaga tcccccgggc agcggtgccg aaccgaccgg
    13861 tgccgcgccc gcctcagctt cacgccgccg acggcggctg ccaccgcccc gccctgaccc
    13921 ggcaacgggc acggatggcg gagcaggaag gccagcaacg ctaggcacaa gcccgttccg
    13981 ggtcaagcag cgcctcgccc ggcccggccg ccgctcagcc accagccggc tcgcagcggc
    14041 cggcgagcag ggctgcgcca tcgagccgct ccagccgggc cgcctcgtcc cgcaggtgtg
    14101 tggcgagccg cagcatgccg gccggcggcg tcgtttccgg cgcgccctcc accggcccgc
    14161 cctccgccgc ccggggcagg acgcggttgt ggatgttgcg gtcgagggtg cgggcgatgc
    14221 cgtcgaggtc gcccgcatcg aggccggcat cgagcgcctg cgcccgggcg aggaaggtct
    14281 gcgccgagcg cttgacgtgc ttgccgaccg agaggtcgcc cacaccctgc tcgcggatgt
    14341 tgcggtcgac gtcggcgaag agcacgtcga acaggtcctg ggcgagggtg cggcccgcct
    14401 ccccctcggc ccgcagccgg cgcagcatca gcgacgtgtg ggcgaagatg agctcgagcc
    14461 tgccgtccgg cgtgtccggc acgccccact cggcgtagaa tcgtggctcg cgggcgcggg
    14521 cgacgatgcc atggtagagg gcgaaggcct tcgccaggcg tcgccggtcg gcccggcggc
    14581 tgccgagcca gccgctgaaa cggccgaaca gcccccggct gacgggccaa gtctcttgtt
    14641 cgcgcgaggt catgacttta cttgatacgc ttcccgttca acccacgcgc ggtcgtttgc
    14701 cgcgcatcgg cctcgggcgg tgatccgccg tccgttggag agagccagag accatgccat
    14761 cgaaaccgag cgcaacccat ctcgttctcg ccctggggct cgccggcggg ctcgcggcct
    14821 gcagcccgca ggtgtcgcag cacggctaca cggtcgacga ggcggcggtg cagcgcatcg
    14881 tccccgggat cacgccgcgc gaggaggtgg cacggctgct cggcagcccg tcgaccctcg
    14941 cgaccttcga ggacaatcgc tggtactacg tgacccagaa gctggagcgg ctctcgttct
    15001 atcagagcga gatcaccgag cagaccgtgc tcaccatcgc cttcgacgac cgcggcatcg
    15061 tccaggatgt cagtcatgtg acgatggacg aggcgatgga gatcgatccg aatgccaacg
    15121 tcacgccgac gctcggcaac gagttgaccg tcttcgagca gctgatcggc aatgtcgggc
    15181 ggttcggcga cccggtggcc ggcaagatcg gcggcaagta gccgtcgagg cgggggccgg
    15241 tccgtggcca tccggcgcgc tcgggcgacg cggatggcca cggcgtcgtc accgggtcag
    15301 tgcgccgcga ggacggcgat gatcaggagg gcgacgatgt tgatgatctt gatcatcggg
    15361 ttgatcgccg ggccggccgt gtccttgtag ggatcgccca cggtgtcgcc ggtgaccgcc
    15421 gccttgtgcg cgtcggagcc cttgccgccg taattgccct cctcgatgta cttcttggcg
    15481 ttgtcccagg cgccaccgcc cgaggtcatc gagatggcga cgaagatgcc cgtgacgatg
    15541 gtgccgagca gcatggcgcc gagagtggtg aaggcggcgg cctggccgcc gacccacgag
    15601 atcacgaaat agacgacgat cggggccagc accggcagga gcgacgggat gatcatctcg
    15661 cggatcgcgg ccttggtcaa aaggtcgacc gcccggccgt agtcgggctt cgaggtgccg
    15721 gccatgatgc cgctgttctc gcggaactgg cgccggacct cctcgacgat cgcacccgcc
    15781 gcccggccga ccgcctgcat gcccatcgag ccgaagagga agggcagcat gccgccgagg
    15841 aagaggccga cgacgacgta ggggtcggag agcacgaagg tgacgtcgag gtccgggaag
    15901 tagtgcttca ggtcctggac gtaggccgcg aaaaggacga gggcggcgag cgccgccgag
    15961 ccgatggcat agcccttggt caccgccttg gtggtgttgc cgacggcgtc gagcgcgtcg
    16021 gttgtctcgc ggacattcgc cggcagatcg gccatctcgg cgatgccgcc ggcattgtcg
    16081 gtgaccgggc cataggcatc gagcgagacg acgaggccgg tcagcgccag catggtggtg
    16141 gcggccacgg cgatgccgta gaggccggca ttgaggtagg cgacgaggat gccggcgcag
    16201 atcacgatga ccggcatggc cgtcgattcc atcgacacgg cgaggccctg gatgacgttg
    16261 gtgccgtggc cggtggtgga cgccttggcg atggacttca ccgggcggta gttggtgccg
    16321 gtgtagtact cggtgatcca catcagcgca ccggtgacgg cgaggccgac cagcgtgcag
    16381 tagtagatct gccggccgcc gaaggtcttg accacgacgt cgccgacctt ggcctcgtag
    16441 ccggtgctgc cgaagccgag caccagggcg gtgagcggca gaagcaggac cagcgagatg
    16501 ccggcggcac cccagaagcc cttgtagagg gcgcccatga tgttcttctt ggggccgagc
    16561 ttgacgagga aggtgcccgc gatcgaggcg agcaggcaga tgccgccgac cgccagcggg
    16621 tagatcatca gggtcgcggt cgccggaccc gagaagaaga tggcaccgag cagcatcgtg
    16681 gcgaccacgg tgaccgcgta ggtctcgaag aggtcggcgg ccatgccggc gcagtcgccg
    16741 acattgtcgc ccacgttgtc ggcgatgacg gccgggttcc ggggatcgtc ctcgggaatg
    16801 ccggcctcga ccttgccgac gaggtcggcg ccgacatccg cacccttggt gaagatcccg
    16861 ccgcccagcc gggcgaagat cgagatgagc gaggcgccga agctgaggcc gaccagggcc
    16921 tcgagaatgg tgcgggtctc gacgccgatg atcatcagga tgccgtagta gacagcgacc
    16981 gcgagcaggc cgagggcgac caccagcatg ccggtgacgg cacctgcctt gaaggcgacg
    17041 tccaggccgg cggcgaggcc gttggggccg gaggccgcct gggcggtgcg gacattggcc
    17101 tggaccgaca cgttcatgcc gatatagccc gtcaaccccg agagcacggc gccgatcagg
    17161 tagccgatgc cgacggagaa gccgagaaag acggtgagca ggatgaagac gacgacgccg
    17221 accatggcga tcgtccggta ctggcggttc aggtaggctc gggccccttc ctgcacggcg
    17281 gccgcgatct cctgcatccg cggcgtgccg gtcggcaggg ccaggatgat ctgtcgtgtg
    17341 agaatcccgt acgcgacacc ggcagcgccg cagccgatga tcaggatcag caagaaccac
    17401 tcgaaacccg acatatgtgg ccgtccccgc accaatcgcc cggtccgtcg atcgggcttg
    17461 cccagacaaa atggcccgat ccgtcgatcg ggcattgctc gccacgtccg caacgcatgg
    17521 tgcattgcag ccacgccctc ttaacggcgt ggccatgggc tgtccatgtg catccccgta
    17581 tcgcgcctgc ggcgcagggc cgccggggcg actgtcaccg gcgcggaaac agagcgtttg
    17641 cggacatggg ctgtcgctct tccgatcggt gacccagata gggtcgagcg aagcccttgc
    17701 cctttccgga gtgacagaca tgcagctcgg cctatctctc tcccgcggca gcctcccgct
    17761 cgccgccttc ctgctggccg cgggccccgt cgcggccgag gaggtcttca ccttcgaccc
    17821 gggtcacacg gagatccagt tcgcctacag ccatctcggc aacagccgcg ccttcggcgc
    17881 cttcgagggc ttcaccggcg agctggcgct ggacaccgac gatccggcca ggagccggat
    17941 cgaggtcgtg atcgacgccg cgagcctgcg caccggcgtc gacgccctcg acgggcatct
    18001 caagagcgcc gacttcttcg cggtcgagag ctcgcccgag atccgcttcg tgagcaccgg
    18061 ggtcgtgccg acttccgaga ccacggccga ggtgaccggc gatctgacga tccgggagac
    18121 gacggtgccg gtcgtcctcg acgtcaccct gaactatctt ggcgaccatc agctcggcca
    18181 gttcgtgccc gattacgcgg acatggaggt ggccggcttc tcggcgcgcg gcacggtgct
    18241 gcgctccgcc ttcgggctgg acatgctggt gccgatgatc agcgacgagg tcgagctggt
    18301 catcgaggcc gagctcttgc ggccgaaggt gctcgtcgac tgagcaggag caccagcagg
    18361 ctgttgaaga agtctcgacg gccgcgacgg gatcgacttt cgtcgctggg aaggcgcccg
    18421 gcgcggggcg atgccatagc atcgtcacgt gtcgggcaac gcacgcagcg gcgaaaagcg
    18481 gcccgtcccg gagggatgcg gtcaggatga ccggcgagct tcgtcggcgc tcgggcgcga
    18541 tgcgcgctgc atcgctggcc ctcacgcctc ctcgcttccg gtcatcctga ccgcatcgcg
    18601 gccatcgcga cttcttcaac agcctgctat ggacgaggat cgaccatgcg cctgaccaac
    18661 tcgccggatg gctacggctt cgtccactgg gccctgcact ggatcactgc ggcgctgatc
    18721 ttctggctct tctggctcgg gctgaccatg gtcgagctgc cgatcagccc gcagatgttc
    18781 caggactatg ccttgcacaa gtcgctcggg ctgaccgtgc tggtgctggt cctcgcccgg
    18841 ctcgcctggc gccggctgac cggcgtgccg gcgctgcccg acgggatggg ccggctcgag
    18901 agcctggtcg ccaccgcggt gcatcacggc atctacgtgc tgctcttggt gattccgctc
    18961 agcggctggc tctattccgg cgccaccgcg agcccggcca actggttcgg cttcttcacc
    19021 gtgccggacc tgatcggcgc caacgaagcc gtggccaagg tgctcagggc cgtgcacgac
    19081 atcgccggca agcttttgat gctctgcctc gccctccatg tcctgggtgc cctcaagcac
    19141 catttcatca gccgcgacgc ggtcctcaaa cgcatgctgc tgccccgaag gagctgatcc
    19201 gatgcgcctg ccagtcgccc tcctggttct cctgaccgcc ttgcccgccg ccgcccggga
    19261 ctggcggatg atcgatgacg agagcagcct cgccttcacg ttcaagcaga tgggctcgcc
    19321 ggtcacaggc aggttcgaga cgttcaccgc cgccatcagc ttcgacccgg cggcccccga
    19381 tgcggcgtcg gtcgaggcgg tcatcgagat cgcgagcgtg gacacgggca atgccgagcg
    19441 cgatgcgggg atcggagggc ccgactggtt cgacgcggcc acctatccga cggcccgctt
    19501 cgtcagcacc ggcttcaccg ccactggcgg cgacagctac gacgtcgcgg gcgagctgac
    19561 catccgcgac gtcaccgacg cggtcgtgct gccgatgacc attgccgtcg acggcgaccg
    19621 ggcggtggcc acaggcaccc tcgagctcga tcgcaccacc ttcggcgtgg gtcagggcga
    19681 ctgggcgagt gcgaccgcgg tcggtcacga ggtcctgctc gagatcgagg tggtggccac
    19741 ggccgaggac tgaccgggaa ggggtcaccg gcaacttgcg cgtttgtggc aatagtcact
    19801 gattcccggg gcgacgcggc gcagtagagg cgcgtcatcg ccgctgggtg tcggctggag
    19861 ccggcgctcc ttgatgcggc gcacgggagt tcgccatgaa gagcgttcag ttcgtctcga
    19921 tcggtcaccg tctcggcatg ggaatgggga agcgcccggc ggggttggcc gacctcgtgg
    19981 cctggctcgg ccgcagcatg cggcggtcgc gcgggcggag cagcctcacc gagctcgacg
    20041 atcgcctgct gcgcgacatc ggcatttccc gggcggaagc gcgcagggaa agcagcaagc
    20101 ccttctggcg ctgaggccgg gtccggccgg ggacggctac agggccagcg agtgccggcc
    20161 ctggcccggc gccaggtagc ggtcgaagca ggcggcgacg aggcgcagca gcggccggta
    20221 gcgggccggc accaggatca cgtcgtcctc gcgacgggcg atgcccagct cgaccagccg
    20281 ggcgaaacgc cccggctccg gggcgatggc ggcagcacca tgcgcgtcgg cgatcgcccc
    20341 gaggtcggcc cggccttcgc acatcaggcg gttgatgatc gcccggcgat gtcggtcgtc
    20401 ggcgctcagc cgcaagccac gggcgatcgg caggcggcct tcggcgattg ccgaccgcca
    20461 ggcctgcagg tcgacatggt tctgcaggta gccatccttg aagctgccga tggccgaggc
    20521 gccgacgccg atcagcgtgg tcgcccgatc ggtggtgtag ccctggaagt tgcgctccag
    20581 ccggcccgcc tcgagggcgc gcgccatcgg gtcgtcaggc cttgcgaaat ggtcgaggcc
    20641 gatcgccacg tagccgagcg cctcgagccg ctcggtgatg gccgcgaact gggcgaggcg
    20701 ctgggccgcg tccggcagca gctcgtcggg gatcatggac tggtgcttct tcagtcccgg
    20761 caggtgggca tagccgaaga cggcgacccg gccgggtgcg agcgccgcca cgctgtccac
    20821 cgtgtcgagc acgctcgcga cgtcctgatg tggcaggccg tataggatgt cgaacgagat
    20881 ggcgtcgatg ccggcgctgc gcagccgctc gagggcgagc ctggtcgtgt cgacgctctg
    20941 gatgcggttg atggcgcgct gcaccgccgg gtcgaaggac tggatgccga agctcgcccg
    21001 gttgatgccg atccgggcga tgcagtcggc gagctcggtg gagacgaagc gcggatcgag
    21061 ctcgatggcc cgctcggcat cggcggtgaa gccgaagcgc tcgtcgatcc gccgcatcag
    21121 cgtggcaaga tcggcggtgc cgaggctcga cggcgtgcca ccgccccagt gcagatgggc
    21181 gagcgggacg ggctcgcgca agtggtcggc gacgaggtcg agctctcgcg ccagggtctg
    21241 cgcgtaggcg ttgatccggc ccgcatgccg ggtgatgtgg gtggtgcagc cgcaatacca
    21301 gcagagctgg cggcagaatg gcacatgcag atagagcgag gccggctggt ccgggggcaa
    21361 agcggcgagc cattccgctg cagccacgga tccgatatcc ggccggaaat gcggtgcggt
    21421 cggatagctc gtgtagcgcg gcacgcggtc ggcttcgaac agccggccga gctgctcgac
    21481 cggcagcgcg ggggccgctt cccctggctg aacggacgtt tcggtgggcg cggccatgtg
    21541 catccccaaa ccggcaaggc ttcccggcat ggctaagccg ccggtgaatc ggttgcgtca
    21601 gggtgccacg ctagcggagc gaggcgcggc cggtcttgat tcaggtcaag gccggccggg
    21661 atcgtggtcc ggctagagcg gcttgggctc gttggccttg gtcgcgacag gcagcgtcgg
    21721 cggcgtgatc acgccgtcgc cggtggtcgt cgcggtcgtt gcggcggccg gctcgggcgc
    21781 cgccttcggc acttcgggga cgggccgcgc cggcggcacg gcgatcgact ggatcggtcc
    21841 gccgtcggcc ggtgccgcgg ccggcacggt gcccggcacg ggctcggccg tctcgcgcgg
    21901 cagcgcctgg gccaccccct tgccgaccag ctcgacgatc gccgagagct gcgggctgtc
    21961 ggtcggtgcc accagcgcga agatcatcgg cccgcgccgc cattgcaggg aaagatggcc
    22021 ctcgggcacg aggcggaacg cgtggtcggc gttcgagcgg gcgacagcgg catagagcac
    22081 gacccggctg ccgacctcgt cggcgtagat caggcggatc gccggcgcgc ggttggcgtc
    22141 gagggtctcg ccgcgaacga gatgcaggcc gatcccgtcg agctcgggcg cactgagcgt
    22201 gaggccgggc acctggccgg cgagccaggt gaccgatgcc aggtcggtgg gcgcggcgtc
    22261 ggtctgggtg acgaggccgg ccggctcgct cgccgccagc tgcgtcgtcc cgaacgggaa
    22321 atagatctcg ggcgcgccag ccgatgaccg cagggcgctg gtgccgctgc cggcgaggcg
    22381 atccagcgtc aacgccgtgc cgccgccgag cacggtcagc gccaccgccg cggcggcggc
    22441 atagcggaag gccgcgacgc gggtccgccg gccggccagc cggcgcacga tctcgccctg
    22501 caggtcggcc ctgaaggtgc ggctgtcatg catcggcaga tgctgcccga gctcgcgcag
    22561 gcgggcattc tggtgcagat aggcctcgac cctggcgacg gtctccgggc gcgaggaaag
    22621 tgcagtcagc acctgcacgc gctggtcgtc gtcgagcgca ccgtcgacga agcggagcaa
    22681 ccggtcattg tcgagcgcgt catcgacgcg cgtctggtct tgcgtcattt caccactcga
    22741 agcttgcgcg aggtctcacg gctggtcatc gagcgcagca cttcccggcc gcgcgacagc
    22801 cgcgacatca cggttcccac aggtatgccc agggcttccg ccgcctccag atagctcagc
    22861 ccctccaggc cgacgagcaa caccacctcg cgctgctcgt tcggcagctt ggccagggcc
    22921 acgacggtgt cccgcagctc gacatgctgc tgctggctgg gtggtgtgac gagcgaagcc
    22981 aggatgtcct cggagaggaa atccgtggcc atgcgtttct cgcgccgcgt cgaatcgacg
    23041 aagacgttgt gcagcgtcga gaagagatag ggacgcaggt cgccgatcgt gtgccagctc
    23101 ttggcgcggg cgagcacccg cgtcagcgtc tcctggacgg catcgtcggc atccgcggaa
    23161 tcgccgagca ggaggagggc atagcggcgc agggccgcca catgcaccac cagctcctcg
    23221 gcgatcgcct gactcgccgc ctccgaccgc atcttcccac gctgccagat catgcgcatt
    23281 cctgttgaaa ctcgatccca tccccattag gccatctgtc gacggaagcg aagtgaaata
    23341 ccggagtgca ggctccggta gaattatcgc cagccgcagc gggctcggtg gcggaagaaa
    23401 atgtccgtcg gcctgtggcc gacggaccaa tcgtgggcgg taaaagccat tgaccgggac
    23461 cgggccggcg cgagggccgg atcggtgtcg gtcaagacac gtgaaataga cgctcgaccg
    23521 tcgaacttat tccggaattt tgctgctgtg ctgccgggtc gatccggtca gtcggggctg
    23581 cgctcgcgta cgtagcggcc gggcgccggc tctatcacct cgaggttgcc ggcgccggga
    23641 tcacgggcgg gcgacgtccc gccgccatga cgctccagcc actgccgcca ggcgggccac
    23701 caggacccct cgtgatgctc ggcccgcgcc agccaggtgt cgggatcggc gctgcggctg
    23761 gtgctcgtcc agtagccgta cttggtcgaa tcggcgggat tgacgatgcc ggcgatatgg
    23821 ccggagccac cgagcacgaa gcgcaccggc ccgccgagca ggcggctgcc ccggaaggtc
    23881 gaccgccagg gggcgatgtg gtcttccctg gtcgagacga agaaggccgg ctgacggacc
    23941 tgcccgaggt cgatgggcac gccgtcgagg atgatgccgc caggcctggt caggaggttc
    24001 tgctggtaca tgcagcgcag gtagaagctg tgcatcatgt agggcatcgc cgtgctgtcg
    24061 gcgttccagt agagcaggtc gaaggcgagc ggtgcccggc ccatcagata gttgctgacg
    24121 acgaaggacc agatcaggtc gttggcgcgc atcaggttga acacctgctg catgtggcgc
    24181 gcctcgagat aacccttctt cgccatgtgc cggtcgagca gctcgagctg ctcgtcgtcg
    24241 atgaagacgg cgaggtcgcc gggctcggcg aagtccagca tggtggtgaa gaaggtggcg
    24301 gacgcgatgc gcttctgccc cttcgccgcc tcgtgcgcga gcaggcaggc ggcgagcgtg
    24361 ccgccgaggc agtagccgat gatgttgacc tcgtcctcgc cggtctgccg ggtgacggca
    24421 tcgagggcgg cgagcgggcc gtccacgagg tagtcctcga agctcttgtg ggcgagctcg
    24481 gccgtggggt tgacccagga gatgacgaag acggtcgagc cctgctccac cgcgtagcgg
    24541 atgaagctgt tcttcggctt gaggtcgagg atgtagaact tgttgatcca gggcgggacg
    24601 atcaggagcg gccgccgctg cacggtcggc gtcaagggcg cgtactggat gagctgcatc
    24661 agctcgttct cgaagaccac cgccccctcg ctcgtcgcga tgttccggcc gagctcgaac
    24721 gcgtcctcgc gcgtcatgct gatcttcagc tcgcccttgc cccgctcgag gtcgtcgagc
    24781 aggcggcgca gcccctcgta gaggctctcg ccccgggtct cggcggcctg gcggagcacc
    24841 acggggttgg tcagcgcgaa gttggagggg gccagcgcgt cgacatactg gcgcgtgtag
    24901 aacgccacct tgctgcgcag cgccgggtcg agcccctcga cattggcgac cgtgccctgg
    24961 atccagctcg ccgtgaccag gtaggactgc ttgatgtagt cgaagacgag atcctcgctc
    25021 caggcgtcgt ccctgaagcg gcggtcgtcg ggcgccggct cgaccatggg cggcacctcc
    25081 tcgcccagca cgcgccgcat gtagctttcc cacaagcggc ccatctcgcg ccaataggcg
    25141 agctgcgcgt cgaccagccg ctcgggccgt gccaggaggt gctcggcgag gcgggcaaag
    25201 gcgtcggtga ccaccttcgg gtcggggatc tggaagtgct cgtcgtcctg ccgctcgagg
    25261 aacctgcgga agatgcggtt gctccgctcg ccgatctcgg ccatacgcgc ggccagttcc
    25321 ctggcgtcgg gcagctcctt gtcgcctgcc atgtcggcgg ttcctccggc tctcagcacg
    25381 gcgcactggc cgcgcggtgc ctcgggggtt ttgcgcggcc cggcgctgca ctgcaagagg
    25441 agacggggat ggacgccatg gacaagtcgg ttgcgacgaa gctggccggg gtcagcacgg
    25501 cgacgctcac cacctgcctg ttcaagcgcg ggctgcgcaa ccagttcctg caggacgtgc
    25561 atccggtggg cggcagcggc acgcggatgg tcgggcccgc cttcacgctc cgctacattc
    25621 cggcgcgcga ggacatcgac gtgctcgacg cctatgccga tttcgagcac ccccagcgca
    25681 aggcgatcga gacctgcccg gccgggcatg tgctggtgat ggacagccgc aaggacgcgc
    25741 gggccgcctc ggcgggcgat atcctggtgt cccggctggt gatgcgcggc gtcgccggcg
    25801 tcgtcaccga tggcgggttc cgcgacaccc cgtcgatcgc cgccctcgcc atcccggcct
    25861 accaccagcg gccttccgca ccgaccggcc cgatcctcca tcacgccgcc gatatcggcc
    25921 tgccgatcgg ctgcggcgac gtcgccgtct atccgggcga catcatggtg ggcgacggcg
    25981 agggcgtcgt ctgcctgccg gcgcacctcg ccgccgagat cgccgacgag gccttcgaga
    26041 tgaccgtcta cgaggacttc gtcgaaagcg aggtcaaggc gggccgatcg atcttcggca
    26101 cctatcccgc gaccgacgcc agccgcgcac gcttcgccga atggcgcaag gcccagggca
    26161 ggtaagaaga agagcaatga ggggggcgtg ggggaaatca tttcccccac atccttcctc
    26221 ataagacctc gattcgctct atccgcccgc agcacagccg gaagatggcg tcctgcggct
    26281 cgtccagctc gagcatggcg gcggccggca ggttcagata ggcgcagcgg atggcccggc
    26341 cgagggcgcc gtgggcgacc gcgaccagcc gcgctgccgg tgccaggtcc tcgagccagg
    26401 ccatcgcccg gtcgaggatc gcctgctgcg tctcgccgcc gccgggtggt gcgacggtcc
    26461 agcgatcgtt gatccgcgcc tgccagagct cggggtcgcg ggcctcgatc tcggcggcgg
    26521 tgagaccatc ccaggcaccc caggtcattt ccttcagccg gtcgtccaag gtgatctcgc
    26581 ggggcgtgcg gccgatggcg cgggcgacga tggcggcggt gtgccgtgcc cggccgagcg
    26641 ggctcgaggt gagcccgtag gccgccggct cgtcgagctc ggccaggagc cgccggccgg
    26701 cgcgctcgcc ctgctcgatg ccgcgcccgg tcagcggcga atcccgatgc ccctggaacc
    26761 gccgctcgag gttccacgag gtctcgccgt ggcggatgag gaagaggtcg gccccgtgca
    26821 tcgagggtca gcgtggggcc ggccggggga caagcggcgg cagcgacgtc ggcaggccgc
    26881 ctgccggtgc tggaatggag gtcggcaact ggcccgtcgg caggggcgcc ggcaccggtt
    26941 ccggaccggt cgccggcctg ggcggcggca atctgtcgag cgtgttcagc tggcgggtgc
    27001 gctcggcccc cggcggctcc tggcgcagct ggtcctgaag attgcgccgc tggagcgtgc
    27061 tcgtgccggc cgggtcgcgg cgcgcccggt cgatctggcg ctccaggctg gggctggtcc
    27121 gtgccgaggg cgagccgaag agcccgtccg ccgcgtccgc cggcagcgag ccaccgccga
    27181 gcacgaccaa cgccaggagg ccgcacgaca cggcagcggc acgggtcggc aacgggaatg
    27241 tgaacgaccg ggagcacccc ccgggacccg ccccggcccc ggcgttcttg acggccgccc
    27301 gcggcgcgtc gactgttgcg tgcatcggca gacccatccc attccgctcg ccaacccgac
    27361 ggaacccttc cccatgtctt ccaaggtcgc gcacagccaa ggcccgggca agagccggcg
    27421 cgatccgcgt ctcgactttt ttcgcggcat cgccatgctg atcatcctcg tggcgcacat
    27481 ccagtggaat tcgtggtcgc tctggatccc ggcacgcttc ggcccgtcgg acgcggccga
    27541 gatgttcgtc ttctgctcgg gctatgcggc ggccatcgcc ttcggcggca ccttcgtcac
    27601 cgccgggttc tggatgggcg tgcggcgcat cgcgctccgc tgctggcagg tctactgggc
    27661 gcatctcggg ctcttcttca ccgtggcggc gatcaccgtc gtcgccaacg agctcttcgg
    27721 gacgccccga tggaactatc tgggcgtgct gaacctcgag cccttcttcg acgacacgca
    27781 gacccagctc gtcgggctct tcaccctcac ctacgtgccg aactacttcg acatcctgcc
    27841 gatgtacctg gtcatcctgg cgatgatccc gctggtgatg ctgctgcacc ggctccacct
    27901 cgccgcagcc atcgccttct gcatcctgct ctacgtcgcc acatgggttt tcaacctcaa
    27961 cctgccggcc gagcccttct cggaccgggt ctggttcttc aacccgttcg gctggcagat
    28021 cgtcttcttc accggcttca tgctgtcgcg cggctgggtc cgaccgccgc cctaccgtcc
    28081 gtggctcctc gccctcgccg ccggctacgt cctggtgatg atcccgatca gccgctggca
    28141 gatctacatg caggtgccgg cgctcgaggc cgtccacgac tggctctggc tcaaggactg
    28201 ggagcagggc ggcatcttca agaccaatca gcaccccttg cgctggctgc atctcctggc
    28261 gctcgcctat ctggcgctct gggtgctgcg cgggcgcgag cactggctcg atacggcacc
    28321 ctgccgcgtg atcatcaagg tgggccagca ggcgctcgcc accttcatga cgagcatggc
    28381 gctggcctgg atcctgtcca tcgccctcga ccagctcggc ggcgagggca cgaccttcgc
    28441 gggcaagtac cggcccggtc gcgagccgct gagcgtggct gccgccaaca tcttcggcct
    28501 cgccgccatc atcggcgtcg cctacatggt cgactggtac aagcgcgagc cctggcgacg
    28561 ggtcggccag gcgccggcac agtccgtcag cgggtcccgg gcagcggcac aaccggcagc
    28621 ggcagctccg gagtcggcgg ccttggcggt cgctccaggc gccagggcgt atcgatgacc
    28681 accccgccca gcgcccgggt tctgggtggc agggcgggcg ggcgatggca gtaggggcgg
    28741 agcgcgccgc agtcccagcg ccagccggcc ggcacgacgc tcatgctgcc gccgcgctcg
    28801 aagcggcagc ggcagagctt gtcggcgatg caggcgacct cgtcggcatt ttccggcgca
    28861 caggacggcg cttgcgcccg cagcgacgct gcggcaagcg agaggaacgc agtgagcagc
    28921 agtagcgtta acaggcgcat gcgtcagttc taccgcatcc gcgttaagga agccttgccg
    28981 tgtcgcccgg gctgacgcgc cgcaccctgt tgctggccgg cgccctgttg ccgctgaggg
    29041 tggccgccgc ggccgagctg ccggtgccgc cgtgcgccgg cgagacgccg cggccggcgc
    29101 cgccgcgcct cgggcagccg ccggccgtgc tcgcctgcac cgacagcgac ctgctcgccg
    29161 cctggcagcc gccggcatgc ctcggctggc gggcgaagcc ggcggacgtc gtcctggcgc
    29221 tggccgcccg gttccggctc gacgatatcg aggcgctgct cgcccggttc accgccatct
    29281 cggcgctcac ccggatccgc tactggtcgg tgacccgcga ggcctggcgc ccgctcttcc
    29341 tcgacgcggt gccgctctcc cgggccgatc cggcggcgac ccggggcgat ttccggcgcg
    29401 acgagatcgt gctcggcgag ccggtccacg tgctgctcga ggacgacctg atcggtgccg
    29461 tcgtgcattc gatcacggtc gaggcgttct cgcccgagct gatccgggtg gccatgcgca
    29521 acgtcctgcc gggcggcgtg ctgggcatgg aggtgctgcc ggtggacggg ctcgagatgg
    29581 tgctgctgct cgagcgggag gagccctcca tctggcgcta ctggtcgatc atgcgggcca
    29641 cgatcgaggc acccgacttc atgctgccgt ccgccgcctc ctacgccaac cgggcggcgg
    29701 cgatctttcg ctacgtggcg agcctgccaa ccgacgccga gccgccgctg gtgcggcggt
    29761 cgcgcgatct tccgagttga gatcgaaacg ggaagcgggc aaaccctgaa tttggctttg
    29821 aaccggggag acggccttga cggtggtgga ttgcgtgctc gacgccaaag cgcaggtcgg
    29881 cgaaggggcg atgtgggacg agaccctggg ccggctctgg tgggtcgaca tcaacgcgcc
    29941 cacgctcaac cgcttcgatc cggcgaccgg cgagaaccgg gcctggacca tgccgtccag
    30001 gatcggctgc ttcggctttc gggccaaggg cggcttcgtg gtggcgctcg aggacggctt
    30061 ctcgaccttc gatcccgaga ccggggcgat cgaggcgatc gtcgatcccg agcccgaatg
    30121 gcccgagaac cggttcaacg acggcacgcc cgattcggcc ggcaggctga tcgccggcac
    30181 catgccgatg ggcgagcggc tgccggtggc ctccatctgg cggctcgatg cggatcgccg
    30241 ggtgagccgg ctggtcgacg ggcttcgggt ctcgaacggg atggccttca gccccgatgg
    30301 ccggctcttc tactggtccg attcggaccc ctcggtgcag accatctggg ttgccgacta
    30361 cgacccggcg agcggcacgc tcggccgacg gcgggttctg gcgaagaccg acgagctcgc
    30421 cgggcggccg gacggcggca cgatcgacgc cgacggctgc tactggatgg cgggcgtcgg
    30481 cggctggcag ctcgtccgct tcacgcccga tggccgggtc gaccgcatcg tcgacatgcc
    30541 ggtcgagcgg ccgaccaaga tcgccttcgg cggcgccgac ctgaagacgc tctacgtcac
    30601 ctcgatcagc ggcgggcaga cgcccggcac cgaggcccgg cagccacagg ccggcggcat
    30661 cttcgcgctt tccgtgccgg gcgtgcaggg gctgccggca tggcgcctgg ccgtctaggc
    30721 cgaccggcca aacgaccgac gcccgagcgg ctgcggcggc gggcgctcgc ctatctcgag
    30781 cggttctcga ccagccgggc gcatctgggc acggtgctgc ggcgccgggc gctgcgcgag
    30841 gccgagacgc tcgagctgga ggcggcaccg gtcgaggccg cgatcgaggc cctgctcgac
    30901 gacctcgtgc ggctcggcct cgtcgacgac cgggccttcg ccgaaacccg cgcccggcgg
    30961 ctggtcgaga agggccggcc ggcccggcgc atcgtccagg agctggcagc caagggagtc
    31021 gatcgcaacg tggccatggg cgtcctggaa gggctcggcg aggagacgcc cgacctcgac
    31081 cttgcggccg ccctggcctt cgcccgccgc cgccggctcg ggccctgggc cgtgccgggc
    31141 ggacgcgagc ggacgccgga gcaggcgctc gccgccttcg cccgggcagg cttcccttat
    31201 gccgtggcgc ggcaggtgct ggaggccgcg agcctggacg agctcgaggc agaagtgcgc
    31261 gacgcctgag ccttgttcgc cagtcgcggc acccgtcgac gttttggcgc tgcggatcga
    31321 cgccgacttg tggtctactc gcctctggat gcggcgccgg gcgccgcgga agccgcgacg
    31381 ggacctgaaa tgtgctgggg cgtggcggcg actgcgacca tgctcggcct cggcacggct
    31441 gccgccgtcc tgacgctgcg gcgcggcgag ccgccggccg tgccgctgac cctcgcctat
    31501 ttcgcgctga tggaagggct gcagctcgcg ggctacgccg tcgtcggcca gtgtggcgag
    31561 cccgtcaatc gggcggtgac cctgctcgcc tacctgcaca tcgccctgca gccgctggtc
    31621 atcaacgcct tcgccatgga gctggtgccg ggggagcgca aggtggcggc gcgacgctgg
    31681 gtctacgcgc tatgcgggct ggcctcgctg ttcatgctcg tgcagctcgt gcccctggag
    31741 cgggccggcg tctgcagcat cggctcaccg ctgtgcggcg ccgagctctg cacgatctgg
    31801 caactggcac atcggctgga gcgtcccgta caacgggctg accgtgccgc tcgaggagct
    31861 ggtcggtccg ggcggcgcct ttccgaccta tatgctcgcc gtcttcctgc tgccgctcgc
    31921 ctacggtgcc tggcgcttcg tgctcttcca catcgcggcc ggcccgctcc tcgccagcac
    31981 cctgaccgac aatccgaacg agatgccggc gatctggtgc ctgttctcgg tcggcattct
    32041 gctgatcgcg ctgagcccgg cgatccgcag ccgcttcggg caggccggaa gctgggctcg
    32101 gccggcggcg ggatgagccg tcgcgtctgg cgcagggcgc ctactccagg ctcatgcgcg
    32161 cctgcagctc cgccgtcgtg ttccggctcg gatagggctc cgccggcacc ggcacgccga
    32221 gatgggcgca gagcggctcc cagccgtcgc cgagccggtg gaccagcagc cggtcggctg
    32281 gcaccgtggc gatgacctcc tcgacatgtc tctcatagac ggcaatcgcg ttctcgcggt
    32341 cgtccggccg gccgccgaac acgttctcac ccacccagtg aatcagcggc gacttctcga
    32401 tgggccagcg ccagaccttc aggatggtct tctcgaagct cgcccaccag ctctcggccg
    32461 tacgccaggt caggatcacc ttggcttgcg gattgagttc gatcagctcg cgccagaaat
    32521 aggccgacgg ccagtccatg cacgcgcgat agccgtcgaa gagcgcgtcc cagtcgggct
    32581 cggcaccccg ggccagctcg atccagagcc gatacagctc gggccggtcc gagagctcca
    32641 gcatgtgatg ggtggggccc tggcccagca tgttcagggc cgtgcgcatc gagtccgtcc
    32701 cggtccttcc gaacccggtg ccgatcacct gcaacggcat caccgatact ccaccgaaga
    32761 caccagccgc acattcgacg ggcatcacgc tactgccgcc gcaagtgccc ggcaagaagc
    32821 atgagggctc agatcagccc ggcgctcttg aagctgggat ggcggtcttg ccgacgatga
    32881 cgtggtcgtg gacggtgaag cgcactgtgg ccttgaccgt cccctttggc caacggctag
    32941 ggaggccgac cgagggcggg aacgtcaggc actcgccgcc ctggccagga cttcatcgag
    33001 ctccggcagc atcacgatgc cgtcctggtc gctgccggac gaatgcacca cgagccagac
    33061 gcagggggcg ccgctgtcgt tgcacggcgc gtgcggcagg tcggcgggca cgaagccctg
    33121 ctcgccggcc gagagctcga cccggtgctc cagacgctgg ccgaagcgga ccgtgcagcg
    33181 gccctccagc aggtaggcga tcgtctcgat gccctcgtgg tagtgcgccc tggccctggc
    33241 accgggcggc atgggcagca cgttcatgca gatcctcgtg gcacccaccg tctcggccga
    33301 ggcacccgca ccataggtga agccctgctt gcccacatag gtgccgccgg gcttgacgat
    33361 cgcaatccgg tcgggatcga gcagggtcat ggcgaacgct ccgcatgggt cgaggggggg
    33421 cggggccatg gcgagggtag ggctgccggc cgcgttgggc caagcggttt cggccaagcg
    33481 cggcgtgctg gaaaccgaaa actcgcttgg ctatgaaggg ggggcgcctc tcttcggcga
    33541 aaaggtcaaa agtgaggtga cctccttttc cggaggtttg atgtgtaaaa tgccatcaag
    33601 catcaacgtt ttcaaagacg cctacctcaa tgctattcgt gatgttattt tcaagaatcg
    33661 actaagcaat tttttggatg aattcccctg gctgacgggc catctcggaa atccttttgc
    33721 tcccgtatgg tttattggcg agaacccgag tcgaagtcgt gtcgaacaca tagataaggt
    33781 ttcgacttca cattcagaga atcttcaatg gagttataaa tttcgggagg ctcggttgtt
    33841 tcgcgatgca cttactaaag ctggccttaa agaaggcgat ccagcagagg acattggatg
    33901 gaactgctat attaccaatg tgaccaaaga acccgaagtt gtaaaaaaaa agaaataatg
    33961 aaaagggtat aatatctgga ttctacaaaa aagaatccgc catttggctg gatgttttac
    34021 aataccaaat tgactttggg agtccgcgtg ttttagtaga actcggttcc gaagctgcac
    34081 ggctgttaga cgtcatgaag aagtgtgggc tgaggactcc gcctatcgag aaaattcccc
    34141 attattctta cgtaatgctt aggccggata gtaaaaccaa gttgggcccg gggcatccgg
    34201 agcggcaggc agaattctca aacaagataa gaactattgc agaaaaattc ggcactggct
    34261 tagtggaaca cgtcccttct tgaccatcac ccccccaagg ccgccatggc ggtgtcgggg
    34321 gccttgtcga tgatgcggag gagggcgcgg gcggggcctt gggggaggcg cttgccctgc
    34381 tcccagttgc gcagggtctc ggcggagacg tcgaggcggc gggcgaaggc ggcttggctc
    34441 aggccggtgc gggcgcgcag gccgcggatg tgggcggcag cggcggctct ggcctctgcg
    34501 tcgtcggcgg cgatctgccg ggcgatctcg gccgcgtcgg cggcgtccag cctggcccgg
    34561 tcgatctcgg gcacgagcgg tgtctcgctg ccgtcggcgg cgatcaggta gacccggtca
    34621 ccgttgatcc gggcgcgaac ggtagtgatt gtgctcacgg cggttggcct ttcccgcaga
    34681 gatgatgcgc gggtgctctt cgcctcgtcc cactccacct cgacagcctg cactatcggc
    34741 caatgaccga ctcggtcaat ggccgaggcc gccgaagtat cggtgggtcc agggagggtt
    34801 gtatagaccg gagacaaggt ttacatgcgt tcgaggacat gggtgacagt tgg
//
LOCUS       DAOTFI010000011        33451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1450_length_33451_cov_6.902982, whole genome shotgun sequence.
ACCESSION   DAOTFI010000011 DAOTFI010000000
VERSION     DAOTFI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 33451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33451
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1450_length_33451_cov_6.902982"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..944
                     /locus_tag="P5558_01895"
     CDS             105..944
                     /locus_tag="P5558_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY23093.1"
                     /translation="MNRARRSLTAEAAAHAVLIAYTIIALFPVVLILINSFKDRRAIF
                     QAPMAPPTPESLSLIGYATVLEQGNFALYFMNSLTVTVLALVLVLLLGAMAAFALAEY
                     RFFGNALMGLYLAIGIMIPIRLGTVGILEMMVATGLVNTRTALVLVYTANGLPLCVFI
                     LSEFMRNVSKDLKDAARIDGLSEYTIFFRIVLPLVRPAMATVAVFTMIPIWNDLWFPL
                     ILAPGEAVKTITLGTQVFIGQFVTNWNAVLAGLALAILPVLALYLVFSRQLIRGITSG
                     AVK"
     gene            992..2059
                     /gene="ugpC"
                     /locus_tag="P5558_01900"
     CDS             992..2059
                     /gene="ugpC"
                     /locus_tag="P5558_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY23094.1"
                     /translation="MAELRIADVEKSFGKVEVLKGVDLAIQDGEFIVLLGPSGCGKST
                     LLRIIAGLEDQTSGTISIGGTTVDHLPPARRGIAMVFQSYALYPHLDVAGNWALGLKQ
                     AGETKEVIEERTKEAARILELGPLLQRRPSELSGGQRQRVAIGRAIVRHPGVFLFDEP
                     LSNLDAALRAQVRFEIGRLHKQLGATMVYVTHDQVEAMTLADRIVVMHQGVVQQVGSP
                     ADLYRTPANLFVAGFIGSPRMNFLKGTARQPGSLELDHGASLPLAELRQPAGEVVLGV
                     RPDGFAVGDGATAPVRGKVVLNEYLGRESYLHLELEAGGRVVVEVAPDNPVRLDEIVG
                     LELKPRAAHLFDAASEQRLAA"
     gene            2063..2818
                     /locus_tag="P5558_01905"
     CDS             2063..2818
                     /locus_tag="P5558_01905"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY23095.1"
                     /translation="MEQAIATDPRLASLRPDPAGTGPLYLQLAERLEAAVAGGLLRDG
                     EPLPPERVLALSLDVSRTTVRRALDELARRGLVTTRHGSGSFVASRFEQPLARLSSFT
                     EDMAARGRSSGFRWLERGRGRASPDETVALNLAPGDDVSRFVRLRFADDEPMCIERAS
                     IPARYLPDPERVETSLYAALRDLGLEPVRALQHLRAAAVGPADAALLSIPPGSPVMAT
                     VRHGYLADDRPVELTRSLYRADRYDFVAEMRRN"
     gene            2830..3930
                     /locus_tag="P5558_01910"
     CDS             2830..3930
                     /locus_tag="P5558_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY23096.1"
                     /translation="MTETHTLEGRIVTPGGVVRGRLQHDSRILSIEPDASASEERLIV
                     PGFIDLHVHGGGGADVMDGADAVRTMARFHARQGTTSLLATTVTAPADELRRAFAGIA
                     AARAEPATNGAEVLGVHLEGPFLNPEALGAQPPFAIAPDLDLVAGLHEAAPIRVATMA
                     PEIDPDGRLLAWLVGHGIRAQIGHTRCSYGEAAAALDRGAVGFTHLYNAMTGLHHRAA
                     GAVGCALARARFAELILDFQHVEAGAALAALRSIPGLYAITDAVAACGMPDGAYHLGR
                     HPIQKSGPTVRLADGTLAGSVLTMDRAFANFAALGLDQVEGVRRTSSIAADYLGLDDR
                     GRLVPGRRADLVLIDARGAVTGVIREGRPIGA"
     gene            4100..5473
                     /locus_tag="P5558_01915"
     CDS             4100..5473
                     /locus_tag="P5558_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY23097.1"
                     /translation="MRVLNSRSRLAKGMSVMLLLGIAGCAGQTELPLLPAEGGGETAA
                     DGAPLTDSGRLVRMGQRSLAAGDPTTAVGFLEQALAVDRDNREAAVLLGNGNLGLGRP
                     QDAAAAFGLVLQDDPADREAAIGYAKAMIAIGRSETAEAHLEPLVRQRPGDVEASNLL
                     GVALDLQGAHDQAIAVYHGSLDLSPGEPALMNNLGLSLALAGRHDEAIAVLRPLAEGY
                     ASSARTRQNLALAYGLRGEMAAAERWSRMDLADSDVTNNLRYFRTIAGLEPGAVRSAA
                     LQPDFKEPVVREAPGEPRLAAPRPAAPVVPAPPSPATSAPAASAPAASAAPKPLVTGP
                     AHPQAPAAAGPAPLVQEPKSEPAAAGPGFGMVEPSVGLVGVEATALGRWFVDLGSFDT
                     ATATSAYWRTLRRTHAAATAGLGRLAGGSTEAVPLIVGPFATAAEADALCAELTGAVV
                     YCNPVQL"
     gene            5561..5755
                     /locus_tag="P5558_01920"
     CDS             5561..5755
                     /locus_tag="P5558_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRY23098.1"
                     /translation="MTTFKRFLADESGATAIEYGLIAALVSVAVLAILGTLGGQLQET
                     FQNISDELTAAGITGGGGGE"
     gene            5853..6401
                     /locus_tag="P5558_01925"
     CDS             5853..6401
                     /locus_tag="P5558_01925"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRY23099.1"
                     /translation="MSLSLLSLAQLGLGIAALTTVVMAAWSDLRTRLIANRLPAILFA
                     LAIPCHALGTTDWQQALASGGSALALAVIVFLLGYALWRLGGVGGGDVKLLVAATFLV
                     GTGGFATLLVGTAFTGGLLALLYLVVPTRLPLVGYHLDAVTAATMTGTGEAVARPGIP
                     YGIAIASGTAFAIVPTLPLIIG"
     gene            6411..7289
                     /gene="cpaB"
                     /locus_tag="P5558_01930"
     CDS             6411..7289
                     /gene="cpaB"
                     /locus_tag="P5558_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HRY23100.1"
                     /translation="MRRILLPLVAVALAIGTALYANAWLEGERRAVLAQVSPVTIAAE
                     PEFIEVLVVAERLTPGSFVKPTSWRWQKWPAVEVPESYLLRGKIDAAELEGSVARQIL
                     VPGDPILADSLVRPGDRGFLAAVLDPGMRAVSVPVDEASSNAGLIFPGDRVDLILTQT
                     IAGDADGAADRRVSETVLENLRVIAMGRRLHSEPGENADGGQVRTVTLEVDPAGAETV
                     ALVTELGKLSLSLRSIASDPATASGSDGPTVPRQTVTWDSDVSRVLRSDDHVRPRLTV
                     MRGAAMERAPLDAESN"
     gene            7289..8731
                     /locus_tag="P5558_01935"
     CDS             7289..8731
                     /locus_tag="P5558_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HRY23101.1"
                     /translation="MRFTLPTPARLAAQLLARLVGRAGSVTPMLAATLALTLGALSIQ
                     PAAAQAVGGDVLGAADRLGLEVRQGKLVRLDRPAASVFVADPDIADVQAHSASLVYVF
                     GRRAGVTTLFAVDGDENLLFSSVVTVEHPVTRMRQVISSLGLAASVDVQSIDGGVLLT
                     GDVADPATAEDLRELVTQFVGENEVVLNRLSIRAPTQVNLRVRVAEMSRDVTKIFGIS
                     WDNALAFGDFALGLSTGRPFAAANTVSGSFENDNLTIDGVIDALEREGLVTVLAEPNL
                     TALSGETASFLAGGEFPVPVGTENNDIQIEFKQFGVSLSFTPTVLSRNRIGLRVRPEV
                     SDLSENGAITLQNIVIPALATRRAETTVELGSGQSFAIGGLISNSTRNSLEKVPGIGD
                     LPILGTLFRSTSFRQRETELVIIVTPYLVEPRAERDLALPTDGFRAPTQLELILDGRL
                     AGSYPRPGPTARSTAVDGLRLAGPAGFVID"
     gene            8743..9465
                     /locus_tag="P5558_01940"
     CDS             8743..9465
                     /locus_tag="P5558_01940"
                     /inference="COORDINATES: protein motif:HMM:NF021025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HRY23102.1"
                     /translation="MTFPTMLAQRVGLCLAVAWLSACSANSPFPPAAPPPPLPQRPTA
                     QFAPLELRHSVLFATGDDRLAPAEAAALAEFVAILDQGLVIDQIVVGHADIRADDALN
                     DALSARRAARVEEVLLEHGIAPERISSAALGRRFPVEAASAAESLRLSRRVEILVNGF
                     VVVEPACPDWSRSVSQDGTNLPMSNFGCANEVNFLRMVADPADLVGPRPLGDVDPVRE
                     VGAVQRYRQDKVRSLKVEAAGP"
     gene            9462..10676
                     /locus_tag="P5558_01945"
     CDS             9462..10676
                     /locus_tag="P5558_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HRY23103.1"
                     /translation="MSTQSLAPASSKKPEDAQFTAYVVDDASREIIDQVARELVIPRA
                     SIRKGSIRTAIDALGQQRSPKILIVDLSNSELPLSDVNELAEVCEPGVTVIAIGDRND
                     VGLFRELLNSGVSDYLVKPLSTALVQRSLLATVDGEARARQTNRLGRLVATVGTRGGV
                     GTTMLATSLAWTIANDRRRRVALLDLDLQFGSVALALDLEPTHGLREALENAGRIDSL
                     YIDRTMARYSDTLFVLSAEEEIGDAVDIDPRALELLVSELRNKYHYLIADLPEPMSPM
                     AQGIVQNATHLVLVTDLSLAGMRDTLRISQAALQANASCQLTVVVNRAGEHRAGEIAV
                     AEFEKAIARPIDVIVPFDPKTVAAAMNVGRPVSRDRGRVATAIGRVADRLTGTTSKRD
                     GGLLGRMLQRKG"
     gene            10680..12113
                     /locus_tag="P5558_01950"
     CDS             10680..12113
                     /locus_tag="P5558_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRY23104.1"
                     /translation="MFGRRAAEAPSSEIAVPANLPTPSEASAAPATSLEPRPSGQPPA
                     IRQPAPAARERRTQAAPAAMQVIQNLLYERIDGSAAARLPRDELLRQVLELIGEIVVE
                     QRLSLTANEQAQMGRSIVDDMIGLGPLEDLVQDEGITDIMVNGPQQVYVERRGKIELT
                     DVRFRDRQHVMNIAQRIVSRVGRRVDETCPICDARLEDGSRVNIIAPPLALDGCSISI
                     RKFSKKSITLDVMARQNNLSNELATVLKIAAACRLNVIISGGTGSGKTTMLNAMSQLI
                     DPGERIVTIEDAAELQLQQPHVVRLETRPANLEGHGEILMRDLVKNALRMRPDRIICG
                     EVRGPEAMDMLQAMNTGHDGSMCTLHSNNPREAMSRLENMITMAATTLPTRAIRSQIV
                     GAVNLVIQVSRMRDGVRRVTHVTEIIGLEGDVITMQDLFSFEYHGENRDGSLNGIFKA
                     SELRPHFIKRAAYYGLDRALLAAMTSQ"
     gene            12128..13126
                     /locus_tag="P5558_01955"
     CDS             12128..13126
                     /locus_tag="P5558_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRY23105.1"
                     /translation="MSISADLLLPGVIGFSTLLVAWAASEAVAGGGKARSGERIARAL
                     QQSRSTGRPREADAAGVSVRRGGTPRQFERWVGEALSGITSLSALSGFLLRAGIGASP
                     TSCLITLLGICVVLAGIALLLGQSLLVSVVVLPTGALLLSRFYLARRVASRERQFLAL
                     FPEAIDLIVRGVRAGLPVTEALRTVGEDVRDPVGQIFREIASGIKIGMTLEDALASVA
                     RMMPVAEFKFFAISLAVQQETGGNLAEILSNLSNMMRRRDQVKLKIKAMSSEARASAM
                     IISALPIVVGLIIFYVNPEYLEVLFYDSRGQMLLGSAVFSMLFGSFVISRMIRFDI"
     gene            13123..14094
                     /locus_tag="P5558_01960"
     CDS             13123..14094
                     /locus_tag="P5558_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRY23106.1"
                     /translation="MNALVVTLEAQLGALASLPVVVGLVTIAICALLWSVVRTDFERT
                     ARHRMIERHRADLRSRSLSSRLRHGEEKRAQLLARAIKVLQLMPAERLEAARWRLSGA
                     GMRSKNALLHFYVAKFACSAGGAVLAFFFNLYSEDFTIGLDAFGLTLLGAGIGFFVPD
                     FALGRRRKRRLKALQQGLPDALDLLVICAEAGLSLDAALGRVADEFGAAMPELAEELT
                     LTAVELSFLPDRRQALLNLSKRVDLPAFRGVVTTLLQTEKYGTPLSQTLRTLSSEYRE
                     QRMLRAEEKAARLPATLTVPMIVFILPTLFIVLGGPAFIRIFDQLID"
     gene            complement(15156..16622)
                     /locus_tag="P5558_01965"
     CDS             complement(15156..16622)
                     /locus_tag="P5558_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632232.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HRY23107.1"
                     /translation="MSFVAMLPDRGLPAAWRALARRAISHRIPPDRIDWQGGGNLFAD
                     SPLPTETGPHEVRVGRAFLELAGSVLWHSAPERFDLLYQALWRVDRGEGAPLSAADPL
                     GQRLGLMAKAVGRDIHKMHAFVRFRELPTDGRRRSFGAWFEPAHNTLEPGSTFFVKRF
                     ADMDWLIATPGLTARYAAGRLSFEPGGQRPDLPDDASAALWATYFASIFNPARIKLGA
                     MRAEMPKKYWKNLPETRHIPAMLADAEARVERMREAGATPPRPGAGAVSTRYRATMPA
                     PADLPATLCEAREAAMQCRRCGLCEAATQTVWGEGDSSARLMIVGEQPGDREDLAGRP
                     FVGPAGRLLREAMRGVELDPDAVWLTNAVKHFKFSSRGKRRIHQKPDRREIEHCRWWL
                     GLELALVRPRLALALGATAALALTGDSRPLASRRGTIETGLHGGPVLLTWHPSHILRM
                     PDRAGQTRLRHELEADLAAGRRHVAAAARQSTALLTAD"
     gene            complement(16619..17848)
                     /locus_tag="P5558_01970"
     CDS             complement(16619..17848)
                     /locus_tag="P5558_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HRY23108.1"
                     /translation="MKRSLQDRLAILSDAAKYDASCASSGGEKRDSRKGGIGSSGGSG
                     ICHAYTPDGRCVSLLKILMTNFCIFDCAYCINRVSSNVERARFSVDEVVTLTLEFYRR
                     NFVEGLFLSSGVIRSPDQTMADMVRIVRTLREGHQFKGYVHLKTIPDAAPELIEEAGR
                     WADRLSINIELPLDASVRRLAPEKRPETIRTAMAQVRLAREAARERTHTGRKAPRFAP
                     AGQSTQMIVGADGADDVAILNASSRLYSGYRLKRVYYSAFSPIPDASAALPLVEPPLL
                     REHRLYQADWLIRFYGFSAGEIATGALAGHLDLHVDPKLAWALQHRAEFPLDVNRAAR
                     DRLLRVPGFGVRTVDRILEARRHGPLRFADLVRIGASMRKARPFIDLPDWSPGRLIDD
                     ARLRARFAPPPEQLRLL"
     gene            complement(17969..19021)
                     /locus_tag="P5558_01975"
     CDS             complement(17969..19021)
                     /locus_tag="P5558_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRY23109.1"
                     /translation="MSGRTPIEAGHARAAGRSSTTALARRQLLAGAAGCAALALASPA
                     RAAVPTALPLATGDIVGQLTYLVTDGKKTLIDLAVERDLGILSISAVNQGVDVWVPGP
                     ERLVTLPTAHVLPEHQRLGIIVNLAEFRLYFFPTPDAPPIVHTIGIGRDGFSTPLGPT
                     TVVRKQANPTWYPTESTRADRPDLPSAVPSGPDNPLGLFALYLGFPTYLIHGTNKPYG
                     VGRRVSRGCIRMYPSGVASLFQQVPVGTPVRIMNDPIKLGWSAGELYIEVHPDTEQFD
                     QLEVDYRFTRKPPPDVAPRITAKAGAEAGRLAWDVIERELVNRQGIPVQITRPVMANV
                     ESRSKSSLPTGFIGLY"
     gene            complement(19047..19406)
                     /locus_tag="P5558_01980"
     CDS             complement(19047..19406)
                     /locus_tag="P5558_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23110.1"
                     /translation="MQELNQKGEWRARHGSRASLAETRFANVEEKTEMTNRFMMTSAA
                     VGLLLALGACSNSNEMDAMKSQIASAQATANAADQKATQALAEAQAASAAADLAAADA
                     AEASRKADEIFRAGLRK"
     gene            complement(19462..19989)
                     /locus_tag="P5558_01985"
     CDS             complement(19462..19989)
                     /locus_tag="P5558_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRY23111.1"
                     /translation="MRRRDLVGGLALAACGLGTGQALAAAPARRPFRGRVDELRVVKH
                     ERRLYGYAGGVEVLTMNVALGIQPRGPKTREGDGRTPEGRYSIIAARPDSDYYRSLLI
                     SYPNAEDKARARAAGVRPGGQIMLHGLDPAIGAVWRDRHWMFNWTNGCVAVTNAEMDV
                     LWGSVALGTPIEILA"
     gene            complement(20108..21373)
                     /locus_tag="P5558_01990"
     CDS             complement(20108..21373)
                     /locus_tag="P5558_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY23112.1"
                     /translation="MRSSQGRAWLVWSVAAGCYAYGFFQRVAPSVIVDDLMTAFALDA
                     TLLGTLSAAYFYTYAALQIPLGVLIDRFGPRRLLLLGAILAALGGTLFALAGGLATAL
                     LARGLIGAGVGVAYIATLKLVGAWFPLHRFGLLAGLTLAAGTLGAIGAQLPLALAVGT
                     FGWRPVLLVVGIAALALAGLVLAVVRDRPPGTEAPSGTTPPATHALGLAGMLARRETW
                     MLVLITGLTGAPILAFAGLWGVPYLVQVHGLDRTAAASLTSVMLVSWAVGGPALGALA
                     DRLGRRSSLILVIAAANCLLWLPLALIPDLPLVLVVPLTAALGFGGGGMIVAFAVVRS
                     VFGVTAAGRALGIVNSAVLLFGAGMQTAFGAMLDARWNGATAGGTRLYDAAAFGAGFV
                     LCVVAAAIVVIAGLGLRAAERQHDAVIGR"
     gene            21463..22311
                     /locus_tag="P5558_01995"
     CDS             21463..22311
                     /locus_tag="P5558_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRY23113.1"
                     /translation="MSKPVEPCPSEPVEIIKGGDRPALLLCDHAGHEVPRAVGDLGIS
                     RDNLTRHIGWDIGAAAVTRRLASRLGATALLNHVSRLVIDVNRRPRTPTSIPAVSDGC
                     VVPGNEGLDESEVDRRIRDYFLPYHRAIARGIARFRRGGRIPVLVAVHSFTPHLNGED
                     RPWPIGVLWRGDRRLAGPVLEALRRRPNLLVGDNQPYSGQREFGFTITFHAQRTRLPH
                     IMFEIRQDEIADARGAAAYATLIGDCLEPALADAALYTIFDGDNLPESGGIRAWRHAG
                     HISPLG"
     gene            22275..23417
                     /locus_tag="P5558_02000"
     CDS             22275..23417
                     /locus_tag="P5558_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HRY23114.1"
                     /translation="MAPRRPHLTLGVEEEYLLVERATGALAISPPDGFMAACQEALGP
                     QVTHELLQAQVEIGTSVCPDVASVRRELAGLRRTIADCAAEFDLALLAVSTHPAASWR
                     EQRSVDLDRYRLLTEDFQALARRLVVCGMHIHAGVEDDELRIDLMNQTAYFLPHLLAL
                     STSSPFWEGQDTGLKAFRPTIFGDLPRSGIPEPFESFADYQRLLAVMAETGICDDPTK
                     IWWDIRPSARHPTLEMRICDVCTRLEDAITIAAIYQSLLALLFRLRSQNQTWRRYPLV
                     LLQENKWRAQRYGTSGTLADLGRRALVPFGDLMAELVDLLRDEARELGCLAELERVPE
                     IVATGTSADHQLRVHHAAIAAGADAAEARRAVVDWLIVASLEDVPA"
     gene            23479..>23655
                     /locus_tag="P5558_02005"
     CDS             23479..>23655
                     /locus_tag="P5558_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HRY23115.1"
                     /translation="MTEIDAATRTELEAAAFRRLVQHFRDRPDVQNIDVMNLAGFCRN
                     CLSKWYAAAAAERGL"
     assembly_gap    23656..23665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23666..23824
                     /locus_tag="P5558_02010"
     CDS             <23666..23824
                     /locus_tag="P5558_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HRY23116.1"
                     /translation="HDVDVLHVGPELGYEEARETVYGMPYDEWKAKHQTPATPEQQAA
                     FARSHPDH"
     gene            complement(23828..24952)
                     /locus_tag="P5558_02015"
     CDS             complement(23828..24952)
                     /locus_tag="P5558_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRY23117.1"
                     /translation="MIDPVKLTIELIARDTRVPEGNEAQSIQALQMLLENAGFSCHGV
                     DLAPGRPNLVARLDPLGSGAPAIAFTGHLDVVPLGEAPWTRQPFAGEIEAGRLYGRGA
                     SDMKSGVAAIVAAALRHAREGGEAPVEIVLTSAEEVGLEGAKVLADRPGALGRAAALV
                     VAEPTANRPAFGNRGNLWLDVHFKGKTAHASEPEKGRNALLAACLAAERFAAYDFGGA
                     RHPLLGRSTVNVAIMRAGANYNSVPDRARLGLDMRLLPGTDIDALLVELAGLAGPDAS
                     LEEIIRLPGSWTEPTDPWMASAIGIAAGITGANDVMGAVPFNTDGSLLAPAYGGIPSL
                     ILGPGEPGQAHQTDEWCEVARIEEAAAIYLELMRRFTPPG"
     gene            complement(24966..25715)
                     /locus_tag="P5558_02020"
     CDS             complement(24966..25715)
                     /locus_tag="P5558_02020"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRY23118.1"
                     /translation="MSPEAHWRTALPLPRTGSIVVLTGAGISAESGLATFRGAGGLWE
                     GHRAEEVATPEAFARDPALVHRFYNMRRRQLADPAIQPNAAHRALARLARAWRGGCLL
                     VTQNVDDLHERAGSHDVVHLHGELRRVRCTACSEERLWLRDLEAKTPCPACGTFGGLR
                     PAIVWFGEMPLHMERITAALQSCALFLAIGTSGLVYPAAGFVALVRDRGRAHTIELNL
                     EPSAVRSDFAEARHGPAGTTVPALVDELLAL"
     gene            25837..27111
                     /locus_tag="P5558_02025"
     CDS             25837..27111
                     /locus_tag="P5558_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY23119.1"
                     /translation="MAVRQPIWIRRVAAPGAEAFAVLYALESFSRAILTSILPLQALA
                     LLGEPQSVSLVFFLGSLAGLFGSLLIPWLVRRTARRYVYSGGALLLAAAALLLAFDGI
                     LALTLGLACRVLGVVALTIGLSLYIMDVIARHDLAHSEPLRMLYSATAWTIGPALGVW
                     LQLRFGAWLPLLLSAATSLTLFAYFWFLRLTETAVLTTGRANAAPGALQNIRRFASQP
                     RLVLAWLIATGRNCWWSIFYIFTPIYAVTTGLGEMAGGLLVSIGTGTLFLMPLWGRVV
                     RRFGLRRVFITGFTLSGVMTLAIAPSFALPWLGAGFLMAASVAMVVLDACGNRLFFAA
                     VHGRERGNMTAVYSTYRDAADLVTPGTLGLVLRVFDLSAVFVIGGFLMFGVATLARRI
                     HPRVGAVRPPAPALTGVDPAPRMADHPAQTAG"
     gene            27143..28033
                     /locus_tag="P5558_02030"
     CDS             27143..28033
                     /locus_tag="P5558_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRY23120.1"
                     /translation="MATIGFVGLGHMGGAMSRNLMRAGHQLEVFDIAEDAVAALVADG
                     ARGAASPAAVAKGKEIVFTMLPIGKIVEEAVFGPNGIAETIAEGSVVVDMSTILPTET
                     KRIGERLKAQGVGMVDAPVGRTSAHAVTGTSTFMVGGEPADIERVRPLLMAMGEMVTI
                     CGPLGTGAAMKLVNNYISAVVNLATAEGLAMGLKAGLELPVMAEVLSHTPAGRGHITT
                     TWPEKALKDEPSPAFMLDLASKDLSLALDMAAALQVTLATGAVGKQLYASAQANGHGR
                     SDWTTGMFRTLKKMGGVASR"
     gene            complement(28272..29258)
                     /locus_tag="P5558_02035"
     CDS             complement(28272..29258)
                     /locus_tag="P5558_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23121.1"
                     /translation="MRAAFTRLLRGQGEILGLLVFFAVVLVFFGLTTPRFLAAGTFQS
                     IAFQLPELGLLTLAMLLPILSGGLNLAITFTANIAGLAMAWVIQLGGGPEAGLMMVLL
                     GTAAALAVGALAGGVMGVVVAFVGAHPILVSLAMMIFLRGLGEFLTRGGDISGFPPFI
                     ATIGHGTVFGIPLPLLILLVAALLWQVLLGRSRFGFSTYMAGSNLEATRYSGIDTRRV
                     LVLVYVLSGVMSAVAGILMLTRFNSVRVGHGEAYLLITVLACFLGGVDPFGGFGRTAA
                     VVLALVILQTIASGLNLMGAHQHLATALWGVFLIAVMVGRWVWQRWSWRAPG"
     gene            complement(29258..30304)
                     /locus_tag="P5558_02040"
     CDS             complement(29258..30304)
                     /locus_tag="P5558_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23122.1"
                     /translation="MPEPGMPGAGRRWLPRSTEGWLTLVILVFGGVLGLATDTFLTFA
                     NLFDLLNISAFNAIMAVGLLVVLVAGGIDISFAVAASVVQYLTAVTVMQLGGGNWAIG
                     LVLASAFGFMLGAINASLIYYFRIISIVVTIATFNIFFGLLMFFTRGRSIYDLPDWLL
                     DRIIVFEFETPGGDFAFLTLPVVVMMAVVVATWLFLNRSSLGRQLYAMGDNAEGARRV
                     GINIAAMHFLAYGWLGVCAGIGGLMQAHYVQEVVPNALYGRELDVLAAVVLGGARLGG
                     GRGTILGALLGVTLVTMTQNGLNLMGVSPYAFQMVVGLVILVAITMSNLEGLEAFIRR
                     FRSGRARAEAGRQG"
     gene            complement(30297..31808)
                     /locus_tag="P5558_02045"
     CDS             complement(30297..31808)
                     /locus_tag="P5558_02045"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY23123.1"
                     /translation="MRGGATDAAPRILAVEHVSKVFGGVQALVDVDFDVLEGEVHCLA
                     GENGCGKSTLIKIVSGVHAPEPGAVLRFAGERVEGLTPVTARRLGIQVIWQDLALFPE
                     MTVAENIAFEANLGGRPRWVDYGAMRRTAAQVLERLGVTLPTGRPLRELGIAQRQVVA
                     IARALVAEARLVFMDEPTASLTQAETRALFEVVHRLKADGIATVFVSHRLAEVLEIAE
                     RVTVLRDGRMVGVYPAAELTQNRLAELMTGEAHEAVVRAQDRSAGRVVLEVEGLARAG
                     EYEDVSFSLHAGEILGITGLLGAGRTEVAATLFGLTSPDRGDIRLDGRELRLGSNRDA
                     IRSGIAYVSEDRLSLGLVQPQSISRNIAITVLDRLLGLLGLLDPEKERRLVAGWVDRL
                     KIRIGRPEDAVATLSGGNQQRVVLAKWLATEPRLLILDSPTVGVDIGARAGIFQIVRE
                     LAASGLAILMISDEIPELYHNADRILHMRAGRLIGEFQPGHAPIEAIEAAVHA"
     gene            complement(31813..32754)
                     /locus_tag="P5558_02050"
     CDS             complement(31813..32754)
                     /locus_tag="P5558_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY23124.1"
                     /translation="MASTAMAQDMPRMGVVVKIGGIPWFNAMEVGIQEQGKELGWDAW
                     MVGPTSADPALQVRAVEDLIAQGVDAIGVVPNDAEVLEPVLARAREQGIIVITHESPN
                     QQNVDWNFEMASLEGFGEAHAKLFAEATGGEGEYALYVGSLTVPAHNAWADAAVAYLE
                     ANYPNMTLVADRFGVAESVDDSRSTALDLMRAHPELKGFLAFGSQGPIGAGRAVDERR
                     KGGEIAVVGPFSPGQGQRLVHDGVISGGFMWNPMEAGRVFVTIADMLKKGEPIEDGTE
                     IPGLGVVSPDAETHNIIVDALVTLNKDTVDELADMGL"
     gene            complement(32885..>33451)
                     /locus_tag="P5558_02055"
     CDS             complement(32885..>33451)
                     /locus_tag="P5558_02055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY23125.1"
                     /translation="AVVIAMGPWSVLAAAWLALPAVHGLEGHSLVFDDPGAAAAALFV
                     DCPAPGGGQGAPEVIPRPDGTTYVCGLPSRRPLPEDPAAVVADERFLEQLRQMIRGFA
                     PGLAAAPVRHGGACFRPVTEDGLPLIGPVAGLEGAFVATGHSVWGMLNGPATGEALAA
                     LIMDGASATVDLAPFDPARLPALAVQAT"
BASE COUNT         4993 a        12107 c        11433 g         4908 t
ORIGIN      
        1 cggcgtgatg gcctatctct ggggcgtgca gcgtcgcatg cgccgctacc agttctgacc
       61 cgcccccgcc cgaccgcccg ccctccccgg atggagcccg accgatgaac cgcgcgcgcc
      121 gctcgctcac cgccgaagcc gccgcccatg ccgtgctgat cgcgtatacg atcatcgccc
      181 tcttcccggt ggtgctgatc ctgatcaact cgttcaagga ccggcgcgcc atcttccagg
      241 cgccgatggc gccgccgacg cccgagagcc tctcgctgat cggctacgcc acggtgctcg
      301 agcagggcaa cttcgccctc tacttcatga acagcctgac ggtcaccgtg ctggcgctgg
      361 tcctggtcct gctgctgggg gcgatggccg ccttcgcgct cgccgagtac cgcttttttg
      421 gcaacgcgct gatggggctc tacctcgcca tcggcatcat gatcccgatc cgcctcggca
      481 cggtcggcat cctcgagatg atggtggcga cgggcctcgt gaacaccagg accgccctcg
      541 tcctcgtcta caccgcgaac ggcctgccgc tctgcgtctt catcctgagc gagttcatgc
      601 gcaacgtctc gaaggacctc aaggacgcgg cccggatcga cgggctttcc gagtacacga
      661 tcttctttcg catcgtcctg ccgctggtcc gcccggccat ggcgacggtc gcggtcttca
      721 ccatgatccc gatctggaac gacctctggt tcccgctcat cctggcaccg ggcgaggcgg
      781 tgaagaccat cacgctcggg acccaggtct tcatcggcca gttcgtcacc aactggaacg
      841 ccgtgctcgc cggcctggcg ctcgccatcc tgccggtgct ggccctctac ctcgtcttct
      901 ccaggcagct catccgcggc atcacctccg gcgccgtgaa gtagccaccc gacccgcaag
      961 cgggaagaga cccccagtca ggaacaagcc catggccgag ctccgcatcg ccgacgtcga
     1021 gaaatccttc ggcaaggtcg aggtcctcaa gggcgtcgac ctcgccatcc aggacggcga
     1081 gttcatcgtc ctcctcggcc cctccggctg cggcaagtcc acgctgctca ggatcatcgc
     1141 cgggctcgag gaccagacct ccggcaccat cagcatcggc ggcaccaccg tcgaccacct
     1201 gccgccggcc aggcgcggca tcgccatggt cttccagtcc tacgcgctct atccgcatct
     1261 cgacgtcgcc ggcaactggg cgctcggcct caagcaggca ggcgagacga aggaggtgat
     1321 cgaggagcgg acgaaggagg cggcccgcat cctcgagctc ggcccgctcc tgcagcgccg
     1381 cccgtccgag ctctccggcg gccagcgcca gcgggtcgcc atcggccggg cgatcgtccg
     1441 ccatccgggc gtcttcctct tcgacgagcc gctctccaac ctcgacgcgg cgctgcgtgc
     1501 ccaggtccgc ttcgagatcg gccggctgca caagcagctc ggcgccacca tggtctacgt
     1561 cacccacgac caggtcgagg cgatgacgct cgccgaccgc atcgtcgtca tgcaccaggg
     1621 cgtggtccag caggtcggca gcccggccga tctctaccgc acgccggcca atctcttcgt
     1681 cgccggcttc atcggctcgc ccaggatgaa cttcctcaag ggcacggccc gccagccggg
     1741 cagcctggag ctcgaccatg gggccagcct gccgctggcc gagctccgcc agccggcggg
     1801 cgaggtcgtg ctcggcgtgc ggccggacgg cttcgccgtc ggtgacgggg caacggcgcc
     1861 ggtgcgcggc aaggtcgtgc tcaacgagta tctggggcgc gaatcctacc tgcatctcga
     1921 gctcgaggcc ggcggccggg tggtcgtcga ggtggcgccg gacaacccgg tccgtctcga
     1981 cgagatcgtg gggctggagc tgaagccccg ggccgcccac ctcttcgacg ccgcgagcga
     2041 gcagcggctc gcggcctgac ggatggagca ggcgatcgcc accgaccccc gcctcgcctc
     2101 gctgcggccg gatccggccg ggaccggccc gctctatctg caactggcgg agcgcctcga
     2161 ggcagccgtc gccggcggcc tgctcaggga cggcgagccg ctgccgcccg agcgggtgct
     2221 ggccttgagc ctcgacgtct cgcgcaccac ggtgcgccgg gcgctggacg agctcgcaag
     2281 gcgcggcctc gtcaccaccc ggcacggctc cgggagcttc gttgccagcc gcttcgagca
     2341 gccgctcgcc cggctctcga gcttcaccga ggacatggcg gcgcgcggcc gcagctccgg
     2401 ctttcgctgg ctcgagcgcg gccgcggtcg cgccagcccg gacgagaccg tggcgctcaa
     2461 cctggcaccc ggcgacgacg tcagccgctt cgtccgtctg cgcttcgccg acgacgagcc
     2521 gatgtgcatc gagcgggcgt cgatcccggc ccgctatctg cccgaccccg agcgggtcga
     2581 gacctcgctc tatgcagcgc tccgcgacct cgggctggag ccggtccgcg cgctgcagca
     2641 tctcagggct gccgccgtcg ggccggccga cgcggccctc ctctcgatcc cgcccggcag
     2701 cccggtgatg gcgaccgtcc gccacggcta cctcgccgac gaccggccgg tcgagctcac
     2761 ccgctcgctc taccgcgccg accgctacga cttcgtcgcc gagatgcggc gcaactgacc
     2821 gaggccagca tgaccgagac ccatactctg gaaggtcgca tcgtcacccc cgggggcgtc
     2881 gtcaggggac ggcttcaaca tgacagccgg atcctctcga tcgagccgga cgccagcgcc
     2941 tcggaagaac gcctgatcgt gcccggcttc atcgacctgc acgtgcatgg cggcggcggg
     3001 gccgacgtca tggatggcgc cgatgccgtc cgcaccatgg cgcggttcca tgcgcggcag
     3061 ggcacgacca gcctgctcgc gaccacggtc accgcaccag cggacgagct gcgccgggcc
     3121 ttcgccggca tcgccgcggc ccgggccgag cccgccacca acggtgccga ggtcctaggc
     3181 gtccatctgg agggaccgtt cctcaatccc gaggcgctgg gtgcgcaacc gcctttcgcc
     3241 atcgcccccg atctcgatct ggttgccggg ctgcatgaag ccgcccccat ccgcgtcgcc
     3301 accatggcgc cggagatcga cccggatggc cgcctcctcg cctggctcgt cgggcatggt
     3361 atcagggcgc agatcggcca cacccgctgc agctacggcg aggccgcggc agcgctcgac
     3421 cgcggcgctg tcggcttcac ccacctctac aacgccatga ccggtctgca tcaccgcgct
     3481 gccggggcgg tcggctgtgc tttggccagg gcacgcttcg cggagctcat cctcgatttc
     3541 cagcatgtcg aggcgggggc ggcgctggcg gcgctgagat cgatccccgg cctctatgcc
     3601 atcaccgacg cggttgccgc ctgcggcatg ccggacggcg cctaccatct cggtcgccac
     3661 cccatccaga agtccggccc gaccgtgcgc ctcgccgacg gcacgctcgc cggcagcgtg
     3721 ctcaccatgg atcgggcatt cgccaatttc gctgcgctcg gcctcgacca ggtcgaaggc
     3781 gtccggcgga ccagcagcat cgcggcggac tacctgggcc tcgacgaccg gggccggctg
     3841 gtgcccggcc ggcgcgccga tctcgtcctg atcgacgcca ggggtgccgt caccggcgtg
     3901 atccgcgagg gccggccgat cggcgcctga tggcacggca ttgcggcggc cagccgaaca
     3961 atcccgcccg ttaaccaatc cttcaagaaa tagcttcatc ctcggtggct gggctcggtc
     4021 cgtcgaccga cgcccatggg ttgccggccg tcgcgctccg cgcggcaacg cccggtggaa
     4081 ccgccgatgg tgaggtcgca tgcgagttct gaacagtcga agccgcctgg cgaagggcat
     4141 gtccgtcatg ctgctcctcg gcatcgccgg ctgcgccggt cagaccgagc tgccgctgct
     4201 gcccgccgaa ggcggtggcg aaacggcggc ggacggggcg ccactcaccg attccggcag
     4261 gctggtccgc atgggccagc gcagcctcgc cgccggcgat ccgacgaccg ccgtgggctt
     4321 cctcgagcag gcgctcgccg tcgatcgcga caatcgcgag gcggccgtgc tgctcggcaa
     4381 tggcaaccta ggcctcggcc ggccgcagga cgcggccgcc gccttcggcc tggtcctgca
     4441 ggacgacccg gccgaccgcg aggccgcgat cggctacgcc aaggccatga tcgccatcgg
     4501 ccggagcgag acggcggaag cgcatctgga gcctttggtc cgccagcggc cgggcgacgt
     4561 cgaggcgagc aatcttctgg gtgtcgccct cgatctgcag ggtgcccacg accaggcgat
     4621 cgccgtctat catgggagcc tcgatctctc gcccggcgag ccggcgctga tgaacaatct
     4681 cggcctctcc ctggcgctcg ccggtcgcca cgacgaggcc attgccgtgc tgcggccgct
     4741 cgccgagggc tatgcctcct cggcgcgcac acgccagaac ctggcgctcg cctatggcct
     4801 gcgcggcgag atggcggcgg ccgagcggtg gagccggatg gatctggccg attccgacgt
     4861 caccaacaac ctgcgctatt tccgcacgat cgccggcctc gagccgggtg ccgttcgcag
     4921 cgccgcgctc cagcccgact tcaaggagcc ggtggtccgg gaagcgccgg gcgagccgcg
     4981 cctcgcggca ccgcgcccgg ccgcacccgt ggtgccggcg ccaccctcgc cggcgacatc
     5041 ggcacctgcc gcgtcggcac cggcggcctc ggcggcacca aagcccttgg tcacgggccc
     5101 ggcacaccca caggcgcccg cggctgccgg ccccgccccg ctcgtgcagg agcccaagag
     5161 cgagccggcg gccgcgggcc cgggattcgg catggtcgag ccctcggtcg gcctggtcgg
     5221 cgtcgaggcc accgcactcg gccgctggtt cgtcgacctc ggcagcttcg acaccgcgac
     5281 cgccacgagc gcgtattggc gaacgctgcg gcggacgcac gccgccgcga ccgccggcct
     5341 cggccgcctc gccggcggct cgaccgaagc tgtcccgctg atcgtcggcc cgttcgcgac
     5401 ggcggccgag gcggacgcgc tctgcgccga gctgaccggt gccgtcgtct actgcaaccc
     5461 ggtgcagctc tgatgcgccg tgacgttcgc cgcttcacgg tttcctaacg attttgcttc
     5521 agactaccgc ctgatcgacg cagcagaagg agtactgcag atgaccacgt tcaagcgttt
     5581 cctcgccgac gagagcggtg cgaccgccat cgagtacggc ctcatcgccg ccctcgtctc
     5641 ggtcgccgtg ctggcgatcc tcggcaccct gggcggccag ctccaggaga cgttccagaa
     5701 catctccgac gagctcaccg cggccggcat caccggcggt ggcggcggcg aatgacgcca
     5761 gcgcgtttgt gacggcagcc ggctggtcga gcgcaggccc ggccagccgg ctggtcgaac
     5821 ccgggcggat cacggcatcg gtcgagaaca gcatgagcct ctcccttctc tccctggcgc
     5881 agctcggcct cggcatcgcc gccctgacca ccgtcgtcat ggctgcctgg tcggacctgc
     5941 gcacgaggct gatcgccaac cggctgccgg ccatcctctt cgccctcgcc atcccctgcc
     6001 atgccctcgg caccaccgac tggcagcagg ccctcgcgtc cggcggcagc gccctcgcgc
     6061 tggccgtcat cgtcttcctc ctcggctatg ccctctggcg cctgggcggc gtcggcggcg
     6121 gcgacgtcaa gctcctggtg gccgcgacct tcctcgtcgg caccgggggc ttcgcgaccc
     6181 tgctcgtcgg caccgccttc accggcggcc tcctggcact cctctacctg gtcgtgccga
     6241 cccgcctgcc gctcgtcggc taccacctgg acgccgtgac cgcggccacc atgaccggga
     6301 caggcgaggc cgtggcgcgg cccggcatcc cctacggcat cgccatcgcc tcgggcaccg
     6361 ccttcgccat cgtcccgaca cttcccctga tcatcggctg aggacagcac atgcggcgca
     6421 tcctgctccc cctcgtcgcc gtcgccctgg cgatcggcac ggcgctctac gccaatgcct
     6481 ggctcgaggg cgagcggcgg gcggtgctgg ctcaggtctc cccggtgacc atcgccgccg
     6541 aaccggaatt catcgaggtg ctggtcgtcg ccgagcggct gacacccggc agcttcgtca
     6601 agccgacctc ctggcgctgg cagaaatggc cggccgtcga ggtgccggag agctacctcc
     6661 tgcggggcaa gatcgacgcg gccgagctcg agggctcggt cgcccgccag atcctcgtgc
     6721 ccggcgaccc catcctcgcc gacagcctgg tgcggccggg cgatcgcggc ttcctcgccg
     6781 cggtgctcga tccgggcatg cgggccgtgt cggtcccggt ggacgaggcc tcgagcaatg
     6841 ccgggctgat ctttccgggc gaccgggtcg acctgatcct gacccagacc atcgccgggg
     6901 acgccgacgg tgccgccgat cggcgggtca gcgagacggt gctcgagaat cttcgggtca
     6961 tcgccatggg ccgccggctg cacagcgagc cgggggagaa cgccgatggg ggccaggtcc
     7021 ggaccgtcac cctcgaggtc gatccagccg gggccgagac cgtggcactc gtcaccgagc
     7081 tcggcaagct gtcgctcagc ctgcgcagca tcgccagcga ccccgccacc gcgtccggca
     7141 gcgacggacc gactgtgccg cgccagacgg tgacctggga cagcgatgtc agccgggtgc
     7201 tgcggtccga cgaccatgtc cgaccgcgtc tgacggtcat gcgcggcgcc gccatggagc
     7261 gggcgccttt ggatgcggag agcaactgat gaggttcacg ttaccgacgc ccgcccgcct
     7321 cgccgcgcaa ctcctcgccc gcctggtggg ccgggccggg tccgtcaccc cgatgctggc
     7381 ggcgaccctg gcactgacgc tcggtgccct gtcgatccag ccggcagccg cccaggccgt
     7441 cggcggcgat gtgctcggcg ctgccgatcg gctcggcctc gaggtccgcc agggcaagct
     7501 cgtgcggctg gatcgcccgg cagcctcggt gttcgtcgcc gacccggaca tcgccgatgt
     7561 tcaggcgcat tcggcctcgc tggtctacgt cttcggccgg cgcgccgggg tcaccacgct
     7621 cttcgccgtc gacggcgacg agaacctgct cttcagcagc gtcgtcacgg tcgagcatcc
     7681 ggtcacccgc atgcggcagg tgatctcgag cctcgggctc gccgcctcgg tcgacgttca
     7741 atcgatcgat ggcggcgtgc tcctcacggg cgacgtcgct gatccggcta cggccgagga
     7801 tctgcgcgag ctggtcaccc agttcgttgg cgagaacgag gtggtgctca accgtttgtc
     7861 gattcgcgct ccgacccagg tcaacctgcg ggtccgggtc gccgagatgt cccgtgacgt
     7921 caccaagatc ttcggcatca gctgggacaa cgccctggcg ttcggtgact tcgcgctcgg
     7981 cctcagcacc ggccggccct ttgccgccgc caacaccgtg tccggcagct tcgagaacga
     8041 caacctgacc atcgacgggg tcatcgatgc cctcgagcgc gagggcctcg tcaccgtgct
     8101 cgccgagccg aacctcaccg ccctctcggg cgagaccgcg agcttcctcg ccggcggcga
     8161 gttccccgtg ccggtcggca cggagaacaa cgacatccag atcgagttca agcagttcgg
     8221 cgtcagcctg tcgttcaccc cgaccgtgct ctcgcgcaac cgcatcggcc tcagggttcg
     8281 tcccgaggtc agcgatctga gcgagaacgg tgccatcacg ctgcagaaca tcgtcatccc
     8341 ggccctggcg acacgtcgcg ccgagacgac ggtcgagctc ggcagcggcc agagcttcgc
     8401 catcggcggc ctgatctcca acagcacgcg caacagcctc gagaaggtgc ccggcatcgg
     8461 cgacctgccg atcctcggca cgctcttccg ctcgaccagc ttccgccagc gggagaccga
     8521 gctcgtgatc atcgtcaccc cttatctcgt cgagcctcgc gccgagcggg acctcgcgct
     8581 gccaaccgac gggttccggg cgccgaccca gctcgagctg atcctcgatg ggcgtctcgc
     8641 cggcagctac cccaggcccg gcccgaccgc gcggtcgacc gctgtcgacg gcttgcgcct
     8701 ggcgggcccc gccggcttcg tgatcgattg agaggcatcg tcatgacgtt tcccaccatg
     8761 ttggcgcagc gggtcggcct ttgcctggcg gttgcctggc tcagcgcctg cagcgcaaac
     8821 tcccccttcc cgcccgccgc cccgccgccg cccctgccgc agcgcccgac cgcgcagttc
     8881 gcgccgctcg agctgcgcca cagcgtgctc ttcgccaccg gcgacgatcg gctggcgccg
     8941 gccgaagccg ccgcgctcgc cgagttcgtg gccatcctcg accagggcct ggtcatcgac
     9001 cagatcgtcg tcggccatgc cgacattcgc gccgacgatg ccctgaacga tgcgctgtcg
     9061 gcgcgccgcg cggcacgagt cgaggaggtt ctgctggagc atggcatcgc acccgagcgg
     9121 atctccagcg ccgcgctcgg ccgccgcttc ccggtggagg cggccagtgc cgcggagagc
     9181 ctgcgcctga gccgccgggt cgagatcctg gtcaacggct tcgtcgtggt cgagcccgcc
     9241 tgcccggact ggtcccgctc cgtttcgcaa gacggaacga acctgccgat gagcaatttc
     9301 ggctgcgcca acgaggtcaa cttcctgcgc atggtggccg atccggccga cctcgtcggc
     9361 ccccggccgc tcggtgatgt ggaccccgtc cgcgaggtcg gcgcggtcca acgctatcgg
     9421 caggacaagg tcaggagcct caaggtggag gcggccgggc catgagcacg cagagccttg
     9481 caccggcgtc gagcaagaag cccgaggacg cgcagttcac cgcctatgtc gtcgacgatg
     9541 cgtcgcgcga gatcatcgat caggtggccc gcgagctggt catcccgcgc gcgtcgatca
     9601 ggaaggggtc gatccgcacg gcgatcgatg ccctcggcca gcagcgctcg cccaagatcc
     9661 tgatcgtcga tctctccaat tccgagctgc cgctgtccga cgtcaacgag ctcgccgagg
     9721 tctgcgagcc gggtgtcacg gtgatcgcca tcggcgaccg caacgatgtc ggtctctttc
     9781 gcgagctctt gaacagcggc gtcagcgact atctggtgaa gcccctgtcg acggcgctcg
     9841 tgcagcgctc gctcctcgcc acggtcgatg gcgaggcgcg ggcacggcag accaaccggc
     9901 tcggccgact cgtggcgacg gtcggcacca ggggtggcgt cggcacgacg atgctggcga
     9961 ccagcctcgc ctggacgatc gccaacgacc ggcggcggcg cgtggcgctc ctcgacctcg
    10021 acctgcagtt cggcagcgtg gcgctggcac tcgatctaga gccgacccac gggctgcgcg
    10081 aggcgctgga gaatgccggt cgcatcgaca gcctgtacat cgaccggaca atggcccgct
    10141 attccgacac cctcttcgtg ctgagcgccg aggaggagat cggcgatgcc gtggacatcg
    10201 atccccgtgc gctcgagctc ctggtctcgg agctgcgcaa caagtaccac tatctcatcg
    10261 ccgatctgcc ggagcccatg tcgccgatgg cgcagggcat cgtgcagaac gccacccatc
    10321 tcgtcctcgt caccgatctg tcgctggccg gcatgcgcga cacgctgcgc atctcccagg
    10381 cggcgctcca ggccaatgcc tcgtgccagc tcacggtcgt cgtcaatcgg gccggcgagc
    10441 atcgcgccgg cgagatcgcc gtggccgagt tcgagaaggc gatcgcccgg ccgatcgatg
    10501 tcatcgtccc gttcgacccg aagaccgtgg ccgcggccat gaatgtcggc cgtcccgtct
    10561 cccgcgatcg cggccgggtg gccaccgcga tcggccgggt ggcggatcgg ctgaccggca
    10621 ccacgagcaa gcgcgatggc ggcctgctcg gccgcatgct gcaacgcaag ggctgagaga
    10681 tgttcggcag acgcgccgcc gaggcgccat cgagcgagat cgcggtcccg gccaacctgc
    10741 cgaccccgtc ggaggcgtcc gctgcccccg cgacgtcgct cgagccccgg ccttcgggcc
    10801 agccgccggc catccggcag ccggcgccgg ccgcgcgcga acggcggacg caggcggcac
    10861 cggcggccat gcaggtcatc cagaacctgc tctacgagcg gatcgacggc tcggccgcgg
    10921 cacgcctgcc gcgcgacgag ctgctgcgac aggtgctcga gctgatcggc gagatcgtcg
    10981 tcgagcagcg cctgtccttg actgcgaacg agcaggcgca gatgggccgc tcgatcgtcg
    11041 acgacatgat cggcctcggc ccgctcgagg atctggtgca ggacgagggc atcaccgaca
    11101 tcatggtgaa cggcccgcag caggtctatg tcgagcggcg cggcaagatc gagctgaccg
    11161 acgttcgttt ccgcgaccgc cagcacgtca tgaacatcgc gcagcggatc gtcagccggg
    11221 tcggccggcg cgtcgacgag acctgtccga tctgcgatgc ccggctcgag gacggcagcc
    11281 gggtgaacat catcgccccg ccgctcgccc tcgacggctg ctcgatctcg atccgcaagt
    11341 tctcgaagaa gagcatcacg ctcgacgtca tggcccggca gaacaacctg tccaacgagc
    11401 tcgcgaccgt cttgaagatc gcggcggcct gccgcctcaa tgtcatcatc tcgggcggca
    11461 cgggctcggg caagacgacc atgctgaacg ccatgtcgca gctgatcgac ccgggcgagc
    11521 gcatcgtcac catcgaggac gccgccgagc tccagctgca gcagccgcac gtcgtccgcc
    11581 tggagacccg gccggccaat ctcgagggcc acggcgagat cctgatgcgc gacctggtca
    11641 agaacgcgct ccgcatgcgg ccggaccgga tcatctgcgg cgaggtgcgc gggcccgagg
    11701 ccatggacat gctgcaggcc atgaacaccg gccacgacgg ctcgatgtgc acgctgcact
    11761 cgaacaaccc gcgcgaggcc atgagccggc tcgagaacat gatcaccatg gccgccacca
    11821 ccctgccgac ccgggccatc cgctcgcaga tcgtcggcgc cgtcaatctc gtgatccagg
    11881 tctcgcgcat gcgcgacggt gtccgccggg tgacccacgt gaccgagatc atcggcctgg
    11941 agggggacgt catcaccatg caggatctct tttcgttcga gtatcacggc gaaaatcgcg
    12001 acggctcgct gaacggcatc ttcaaggcat cggagctgcg cccgcacttc atcaagcgtg
    12061 ccgcctatta cgggctggat cgggcgctcc tggccgccat gacctctcaa taggatcgga
    12121 ccaccggatg agcatctcgg cggatctcct gctgcccggc gtcatcggct tctcgacgct
    12181 gctcgtcgcg tgggcggcct ccgaggcggt cgcgggtggc ggcaaggccc gctccggcga
    12241 gcgtatcgcc cgtgccctgc agcagagccg ctcgaccggc cgccccaggg aggcggatgc
    12301 cgccggcgtc agcgtgcgac gcggcgggac gcctcgccag ttcgagcgat gggtcggcga
    12361 ggcgctgtcc ggcatcacca gcctctcggc gctctccggc tttctcctgc gggcgggcat
    12421 cggcgcttcg ccgacgagct gcctcatcac cctcctgggc atctgcgtcg tgctcgccgg
    12481 catcgccctg ctgctcggcc agtcgctgct cgtgtccgtc gtcgtgctgc cgaccggcgc
    12541 tctgctcctg tcacgtttct acctggcgcg acgcgtcgcc agccgcgagc ggcagttcct
    12601 ggcgctcttt ccggaagcga tcgacctcat cgtgcgtggc gtgcgcgccg ggctgccggt
    12661 caccgaggcg ctgcgcacgg tgggcgagga cgtgcgcgat ccggtcgggc agatctttcg
    12721 ggagatcgcc tcgggcatca agatcggcat gaccctcgag gatgccctcg cctcggtggc
    12781 acgcatgatg cctgtcgccg agttcaagtt cttcgcgatc agcctcgccg tccagcagga
    12841 gaccggcggc aacctcgccg agatcctctc caacctctcc aacatgatgc gccgtcgcga
    12901 ccaggtgaag ctcaagatca aggcgatgtc atcggaggcg cgggcttccg ccatgatcat
    12961 ttccgccctg ccgatcgttg tcgggctcat catcttctat gtcaatccgg agtatctcga
    13021 ggtcttgttc tacgattcgc ggggacagat gctgctcggc tcggcggtct tcagcatgct
    13081 gttcggctcg ttcgtcatca gccgcatgat caggttcgac atatgaacgc gctcgtcgtc
    13141 acgctcgagg cgcagctcgg tgcgctggcg tcgctgccgg tggtcgtcgg cctcgtcacg
    13201 atcgccatct gcgccctcct ctggtcggtc gttcgaaccg atttcgagcg caccgcgcgg
    13261 caccgcatga tcgagcgtca tcgcgccgat ctccgatcgc gctcgctcag cagccggctg
    13321 cggcacggcg aggagaagcg ggcccagctg ctcgcccgcg ccatcaaggt gctgcagctg
    13381 atgccggccg agcgcctcga ggcggcgcgc tggcggttgt ccggcgccgg catgcggtcg
    13441 aagaacgcgc tcttgcactt ctatgtcgcg aagttcgcat gctccgcagg cggggcggtt
    13501 ctcgccttct tcttcaatct gtactcggaa gacttcacca tcggcctcga tgccttcggg
    13561 ctgaccctgc tgggcgcggg gatcggcttc ttcgttcccg atttcgccct gggccggcgt
    13621 cgcaagcggc gtctcaaggc gctgcagcag ggcctgccgg acgcgctcga cctgctcgtg
    13681 atctgcgccg aggcgggcct ttccctggat gcagcactcg gccgggtggc cgacgagttc
    13741 ggcgccgcca tgccggagct cgccgaggag ctgaccctga ccgcggtcga gctcagcttc
    13801 ctgccggatc gccggcaggc gctcttgaac ctgtcgaagc gggtggacct gccggccttc
    13861 cgcggtgtcg tgaccaccct cctgcagacc gagaagtatg gcacgccgct ctcccagacc
    13921 ctgcggaccc tgtcctccga atatcgcgag cagcgcatgc tcagggccga ggagaaggcc
    13981 gcacgcctgc cggcgaccct gaccgtgccg atgatcgtct tcatcctgcc gaccctcttc
    14041 atcgtgctgg gcggccccgc cttcatcagg atcttcgacc agctgatcga ctgagcaggc
    14101 gcaccgatca agacccgtca ccgtccgagc ccgtgccggc ccggccatgc tgcccgaccg
    14161 cccgtcccct atcccgacga gccgtccagg ccgatatgcg gctgacccga cccgggtcga
    14221 atcgtggcct ccccggggcg gcgacccttc gcacggcgaa ccacatgacg acggcgcgat
    14281 ccgtggccgg cgtggtcggg atcagtctgc cgtttgatcc aaatggcgac cccgcccccc
    14341 cctcggtcac gtgccaaagg cgcgtcgcgc gccacccggc cggcgggccg ccctcgcttc
    14401 ggccgcgcgc cccgatcccg cctgctgccg tcgggctctt cggcgcatgg gcggcgaagc
    14461 ttatccaatg ggcattgctc gcggcatggc ccagggcctc ggcggcaaag gccgggccat
    14521 cggcaagcga ggtgccggtg gtcgagaaga ggacgcaatc gacgtccagt cgctcgtgct
    14581 cgaggaagac ctccaaaaaa ctgtgcctcc atgccgaggg cacagccgaa gcggacacga
    14641 tccttgtcga aggtcaccgg cgcgctgccc ggcgtgcaca tgaacgaaat ccaggtgtgc
    14701 cacagcaggc gctcgtcgtt gccggtcacg aggtcgccct gagaccacgt ggaggctgtt
    14761 gtgcggccgg atgggcgggg tcagtcgatg cgccgagccg aagaccgccc agaggtggag
    14821 ttcacgggcg accacgaggc gcacggtccg ggcctcggac gcgttttcgg ttctcgaggc
    14881 tccacgaata tgcggcaggg ccgccgtgca ggtttcgcca ggcttcgcga cgcgggcgcg
    14941 cacgcgtcgc ttgagattgc cgtgctctgt catgggcatg gccttccacg tcgcgggcgg
    15001 cgttccccgg caggcgcgcc cgagacggtc ggaccgggaa agcggcccga ggacaaaagc
    15061 ccctttgcct ccgctcgcca ccgtgccggg gaccgcttcc ggaggttcgg cgtgggccac
    15121 gccgcagcca gacatgccgg ggcgatcccg cttcgtcagt cggccgtgag cagcgccgtc
    15181 gattgccttg cagcggcggc cacgtggcga cgaccggcgg ccaggtccgc ttccagctca
    15241 tggcgcagcc gcgtctgccc ggcccggtcg ggcatgcgca ggatatggga cggatgccag
    15301 gtgagcagga cggggccacc atgcagcccg gtctcgatcg tgccgcgccg tgacgccagc
    15361 ggccggctgt caccggtgag cgccaaggct gccgtcgcgc cgagggccag ggcgagacgc
    15421 ggccggacca gggccagctc gaggcccagc caccagcggc aatgctcgat ctctctccgg
    15481 tccggcttct ggtggatgcg ccgcttgccg cgcgacgaga acttgaagtg cttgacggca
    15541 ttggtcagcc agacggcgtc gggatcgagc tcgaccccgc gcatcgcctc gcggagcagg
    15601 cggcccgccg ggccgacgaa ggggcggccg gcgagatcct cccgatcgcc gggctgctcg
    15661 ccgacgatca tgagccgggc gcttgaatcg ccctcgcccc agaccgtctg cgtcgcggcc
    15721 tcgcagaggc cgcagcggcg gcactgcatg gcagcctcgc gtgcctcgca gagcgtggcg
    15781 ggcagatcgg cgggtgcggg catggtggcg cgatagcgcg tcgagaccgc cccggcaccg
    15841 gggcgcggcg gcgttgcgcc cgcttcgcgc atccgctcga cccgggcctc ggcatcggcg
    15901 agcatggcgg ggatgtggcg ggtctcgggg aggttcttcc agtatttctt cggcatctcg
    15961 gcgcgcatgg ccccgagctt gatgcgcgcc gggttgaaga tgctggcgaa ataggtggcc
    16021 cagagcgccg ccgaggcgtc gtccggcagg tcggggcgct gcccgcccgg ctcgaaggag
    16081 agccggccag cggcgtagcg ggcggtgagt cccggcgtcg cgatgagcca gtccatgtcg
    16141 gcgaagcgct tgacgaagaa ggtcgagccc ggctccagcg tgttgtgcgc cggctcgaac
    16201 caggcgccga agctgcgccg cctgccgtcg gtgggaagct cgcggaaacg gacgaaggca
    16261 tgcatcttgt ggatgtcgcg ccccacggcc ttcgccatca ggccgagccg ctgcccgagc
    16321 ggatcggcgg cggagagggg tgccccctcg ccgcggtcga cgcgccagag tgcctggtag
    16381 agcaggtcga agcgttcggg cgccgaatgc cagagcaccg agccggcgag ctcgaggaag
    16441 gcccggccta cccggacctc gtgcgggccg gtctccgtcg gcagcggcga atcggcgaag
    16501 agattgccgc cgccctgcca gtcgatccgg tccggcggaa tgcgatggct gatggcgcgg
    16561 cgggcgagcg cgcgccaggc ggcggggagg ccgcggtccg gcagcatggc gacgaagctc
    16621 acagcagccg caactgctcg ggcggcgggg cgaagcgcgc cctgaggcgg gcatcgtcga
    16681 tcagccggcc gggcgaccag tcgggcaggt cgatgaacgg gcgcgccttt ctcatcgagg
    16741 cgccgatccg gacgagatcg gcgaaccgca ggggcccgtg gcggcgcgcc tcgaggatac
    16801 ggtcgacggt gcgcacgcca aagcccggca cgcgcagcag ccggtcgcgc gcggcgcggt
    16861 tcacgtcgag gggaaactcg gcgcgatgct gcagtgccca ggcgagcttc ggatcgacgt
    16921 ggagatcgag atgcccggcc agggcaccgg tcgcgatctc cccggcggaa aagccgtaga
    16981 agcggatcag ccagtcggcc tggtagagcc ggtgctcgcg aagcaggggc ggctcgacca
    17041 ggggcagcgc cgccgacgcg tcggggatgg ggctgaaggc ggaatagtag acccgcttga
    17101 gccggtagcc gctgtagagt cggctcgagg cattgaggat ggccacgtcg tccgcgccat
    17161 cggcccccac gatcatctgc gtgctctggc cggccggggc gaagcgcggc gccttgcgcc
    17221 cggtatgcgt tctctcgcgc gccgcctcgc gagcgagccg cacctgggcc atggcggtgc
    17281 ggatcgtttc cggccgcttc tcgggggcga gccggcgaac gctcgcatcg agcggcagct
    17341 cgatgttgat cgacaggcgg tcggcccagc gtcccgcttc ttcgatcagc tccggcgcgg
    17401 catcggggat cgtcttcaga tggacgtagc ccttgaactg gtgcccctcg cgcagggtgc
    17461 ggacgatccg caccatgtcc gccatggtct ggtcgggcga gcggatgacg ccggacgaca
    17521 ggaacagccc ttcgacgaaa ttgcggcgat agaactcgag cgtcagcgtg acgacctcgt
    17581 cgaccgagaa gcgcgcgcgc tcgacgttgc tggagacccg gttgatgcag taggcgcagt
    17641 cgaagatgca gaaattggtc atcaggatct tgagcagcga gacgcagcgg ccgtcgggcg
    17701 tgtaggcgtg gcagatgccg ctgccgccgg aggagccgat gccgcccttg cgcgaatcgc
    17761 gcttctcgcc cccgctcgat gcgcaggagg cgtcgtattt ggcggcgtcc gagaggattg
    17821 ccagccggtc ttggagcgag cgtttcatgg cccgcggtca catattgttc cagcggaccc
    17881 cggcaaggct gcggcctgtc ggcggcgcag cgagcgaccg cgtgacgcag tgctcccggt
    17941 gacggccgga tcggccgtca gagagtgctc agtagaggcc gatgaagccg gtcgggaggc
    18001 tcgatttcga gcgactctcg acgttcgcca tgacggggcg ggtgatctgc accgggatgc
    18061 cctggcggtt gaccagctcg cgctcgatga cgtcccaggc gagacggcca gcctcggcgc
    18121 cggccttggc ggtgatgcgg ggggcgacgt cgggtggcgg ctttctggtg aagcggtagt
    18181 ccacctcgag ctggtcgaac tgctcggtgt ccgggtggac ctcgatgtag agctcgccgg
    18241 ccgaccagcc gagcttgatc gggtcgttca tgatgcgcac gggcgtgccg accggcacct
    18301 gctggaagag cgaggcgacg cccgaggggt acatgcgaat gcagccgcgg ctgaccctgc
    18361 gaccgacgcc gtagggcttg ttggtgccgt ggatcaggta ggtgggaaag cccagataga
    18421 gcgcgaagag gccgagcggg ttgtcggggc cggaaggcac cgcggacggc aggtcgggcc
    18481 ggtcggcgcg cgtggattcg gtcgggtacc aggtcgggtt cgcctgcttg cggacaacgg
    18541 tcgtcgggcc cagcggcgtc gagaagccgt cgcggccgat gccgatcgtg tggacgatgg
    18601 gcggggcgtc cggcgtcggg aaaaaataga ggcgaaactc ggcgagattg acgatgatgc
    18661 ccaggcgttg gtgctcgggc aggacatggg cggtcggcag ggtgaccagc cgctcgggtc
    18721 ccgggaccca aacatcgact ccctgattga ccgccgaaat gctgagaatg ccgaggtcac
    18781 gctcgacggc gagatcgatc agcgtcttct tgccgtcggt cacgagatag gtcagctggc
    18841 cgacgatatc gcccgtcgcc agcggcagtg ccgtcggcac ggccgctctg gcgggcgagg
    18901 ccagcgccag cgcggcgcaa ccggcagcac cggccagcag ctgccgccgc gcgagcgcgg
    18961 tcgtggacga acgcccggcc gctcgagcat ggcccgcctc gatcggggtt cgtccactca
    19021 tgccatgaag ctccgtcgcc ctgccgctat ttgcgcaggc cggcgcggaa gatctcgtcg
    19081 gccttgcggc tcgcctcggc ggcatcggca gccgcgaggt cggcggcggc ggaggcggcc
    19141 tgcgcctcgg cgagcgcctg cgtggccttc tggtcggccg cattcgcggt cgcctgggca
    19201 ctggcgatct gcgacttcat ggcatccatc tcattgctgt tgctgcaggc gccgagcgcc
    19261 agcagcaggc cgacggcggc actcgtcatc atgaagcggt tggtcatctc tgtcttctcc
    19321 tcgacgttgg caaatcgtgt ttcggcaagg gatgcccgcg aaccgtgtcg cgcgcgccat
    19381 tcccccttct ggttgagctc ctgcacagga gcagccgcac tataggcaat tgttcttccg
    19441 gtagaagaac gtccaccggt ttcatgcgag gatctcgatc ggtgtgccca gcgcaacact
    19501 cccccagagc acgtccatct cggcattggt cacggcgaca cagccgttgg tccagttgaa
    19561 catccagtgg cggtcgcgcc acacggcccc gatcgccggg tcgagcccgt ggagcatgat
    19621 ctgcccgccg ggcctgactc ctgccgccct ggcccgggcc ttgtcctcgg cgttcggata
    19681 ggagatcagg agcgagcgat agtagtcgct gtcgggccgg gcggcgatga tggaatagcg
    19741 accttccggc gtgcggccgt cgccttccct cgtcttcggg ccgcgcggct gaatgccgag
    19801 cgccacgttc atggtgagca cctcgacgcc gccggcatag ccgtagagcc ggcgctcgtg
    19861 cttgaccacg cgcagctcgt ccacgcggcc gcgaaacggc cggcgggccg gtgcggccgc
    19921 cagagcctgg ccggtcccga gcccgcaggc ggcaagcgcc agtccaccga cgagatcacg
    19981 ccgtcgcatc gttcgatccg tcaggtatca cccttttaat ccaacttccg gtgctggcgc
    20041 cagttcccgt ctgacgcgaa tgtcgtctat cacggcattc ttacgaattc atcaatttcg
    20101 caacgcctca gcgcccgatg acggcatcgt gttgccgctc cgcggcacgc aggccgagcc
    20161 cggcaatcac gacgatcgcc gcggcgacga cgcagaggac gaagccggcg ccgaaagccg
    20221 ctgcatcgta gagccgcgtg ccgccggcgg tcgcaccgtt ccagcgcgca tccagcatgg
    20281 cgccgaaggc ggtctgcatg cccgcgccga agagcaggac ggcactgttg acgatgccga
    20341 gcgcccggcc ggccgcggtc acgccgaaga cgctgcgcac cacggcgaag gcgacgatca
    20401 tgccaccgcc gccgaagccg agcgctgcgg tcagcggcac gacgagcacc agcggcaagt
    20461 cgggtatcag ggccaggggc agccagagca ggcagttggc ggcggcgatc acgaggatca
    20521 gggacgaccg gcggccgagc cggtcggcca gcgcaccgag cgccggcccg ccgaccgccc
    20581 agctcaccag catcaccgaa gtgaggctcg ccgccgccgt gcggtcgagc ccatgcacct
    20641 ggacgaggta gggcacgccc cagagcccgg cgaaggcgag gatgggcgcg ccggtaaggc
    20701 cggtgatcag gacgagcatc caggtctcgc gcctcgccag catgccggcg aggccgagcg
    20761 cgtgggtggc gggaggcgtt gtgccactcg gcgcttcggt cccgggcggc cgatcgcgga
    20821 cgacggcgag gacgagcccg gcgagcgcca gtgcggcgat gccgacgacg agcaacacgg
    20881 gccgccagcc gaaggtgccg acggcgaggg ccagcggcag ctgcgcgccg atcgccccga
    20941 gtgtgccggc ggcgagggtg agcccggcga ggaggccgaa gcgatggagc gggaaccagg
    21001 cgccgaccag cttcagcgtg gcgatatagg cgacgccgac cccggcgccg atcaggccgc
    21061 gggcaaggag tgccgtggcc agcccgccag ccagcgcgaa gagcgtgccg ccgagggcgg
    21121 cgaggatggc gccgaggagc aggagccggc gcgggccgaa gcggtcgatc agcacgccca
    21181 gcggaatctg cagggcggcg taggtgtaga agtaggcggc cgagagcgtg ccgagcagcg
    21241 tcgcgtcgag agcaaaggcg gtcatcaggt cgtcgacgat gaccgacggt gcgacacgct
    21301 ggaagaagcc gtaggcgtag cagccggccg cgaccgacca gaccagccac gccctgccct
    21361 ggcttgaccg catcaccctc gccccggccc aatggtgacg gcagcaccct gcgtccccgg
    21421 cgcgccttgg cggaccggcc gcgggcaggc tacagcaggg ccatgagcaa gcctgtcgag
    21481 ccctgtccca gcgagcccgt cgaaatcatc aagggtggcg atcggcccgc cctcctgctt
    21541 tgcgaccatg ccggtcacga ggtgcccagg gcggtcggcg acctcggcat cagccgagac
    21601 aacctgaccc ggcatatcgg ctgggacatc ggggcggctg cggtgaccag aaggctcgcc
    21661 agccggctcg gcgccacggc cctgctcaac cacgtctccc ggctggtgat cgacgtcaac
    21721 cgccggccgc gcaccccgac ctcgatcccg gcggtcagcg acggctgcgt cgtgccgggc
    21781 aacgagggcc tcgacgagag cgaggtcgac cgccgcatcc gtgactactt ccttccctat
    21841 caccgtgcca tcgcccgtgg catagcgcgt tttcgccgcg gcgggcggat cccggtgctg
    21901 gtcgcggtgc acagcttcac cccgcatctg aacggcgagg accggccctg gccgatcggc
    21961 gtcctctggc gcggcgaccg gcggctcgcg ggacccgtgc tcgaggcatt gcgccgccgc
    22021 ccgaacctgc tcgtgggtga caaccagccc tattcgggcc agcgcgagtt cggcttcacc
    22081 atcaccttcc atgcccagcg cacccgcctg ccgcacatca tgttcgagat ccgccaggac
    22141 gagatcgccg atgccagggg cgcggccgcc tatgccacgc tcatcgggga ctgcctcgag
    22201 ccggctttgg ccgatgccgc gctctacacc atcttcgacg gcgacaacct gccagaatcc
    22261 gggggaatcc gcgcatggcg ccacgccggc cacatctcac ccttggggta gaggaggagt
    22321 atctcctggt cgagcgggcg accggcgccc tcgcgatcag cccgcccgac ggcttcatgg
    22381 ccgcctgcca ggaagccttg gggccgcagg tgacgcacga gctgctgcag gcgcaggtgg
    22441 agatcggcac gtcggtctgt ccggacgtgg cgagcgtcag gcgcgagctc gccggactca
    22501 ggcggaccat cgccgattgt gccgccgagt tcgacctggc gctgctcgcg gtctccaccc
    22561 accccgcggc gagctggcgc gagcagcgca gcgtcgatct ggaccgctac cgcctcctga
    22621 ccgaggattt ccaggcgctg gcccgccggc tggtggtctg cggcatgcac atccatgccg
    22681 gggtcgagga cgacgagctg cgcatcgacc tgatgaacca gaccgcctat tttctgccgc
    22741 atttgctggc gctgagcacc tcctcgccct tctgggaggg tcaggacacc gggctcaagg
    22801 cgtttcgccc gacgatcttc ggcgacctgc ccaggtccgg cattcccgag cccttcgaga
    22861 gcttcgccga ctaccagcgc ctgctcgcgg tcatggccga gaccggcatc tgcgacgacc
    22921 cgaccaagat ctggtgggat atccgtccca gtgccaggca cccgacgctg gagatgcgaa
    22981 tctgcgacgt ctgcacgcgc ctcgaggatg cgatcaccat cgccgccatc taccagtcgc
    23041 tgctcgccct gctctttcgg ctgcgctccc agaaccagac ctggcgacgc tatcccctgg
    23101 tgctgctgca ggagaacaag tggcgcgccc agcgctacgg cacgagcggc acgctcgccg
    23161 atctgggccg ccgggcgctg gtgccgttcg gcgacctcat ggcggagctg gtcgacctcc
    23221 tgcgcgacga ggccagggag ctcggctgcc tcgccgagct cgagcgcgtg cccgagatcg
    23281 tggccaccgg caccagcgcc gaccaccagc ttcgcgtcca tcacgcggcg atcgccgccg
    23341 gtgcggatgc ggcggaggcg aggcgcgccg tggtcgactg gctgatcgtt gcctcgctcg
    23401 aggatgttcc ggcttgaggc tggtgcagcg cgacgccatc tctgggccag accggaccgg
    23461 accacgaaag gtgacaccat gaccgagatc gacgcagcca cccgaaccga gctcgaggcc
    23521 gcggcctttc gccggctcgt ccagcacttt cgcgaccggc ccgacgtgca gaacatcgac
    23581 gtcatgaatc tcgccggctt ctgccgcaac tgcctctcca agtggtacgc ggcggcggcc
    23641 gccgagcgcg ggctgnnnnn nnnnncatga cgtcgatgtt ctgcacgtcg ggccggagct
    23701 cggctacgag gaggcgcgcg agaccgtcta cggcatgccg tatgacgagt ggaaggcgaa
    23761 gcaccagacg ccggcgacgc cggagcagca ggccgctttc gccaggtcgc accccgacca
    23821 ctgaggctca gccgggcggc gtgaaccgcc gcatcagctc gaggtagatg gcggccgcct
    23881 cctcgatccg cgccacctcg caccactcgt ccgtctggtg cgcctggccc ggctcgccgg
    23941 ggccgaggat cagcgacgga atgccgccat aggccggggc caggagcgag ccgtcggtgt
    24001 tgaacgggac tgcccccatg acgtcgttgg cgccggtgat cccagccgcg atgccgatcg
    24061 ccgacgccat ccagggatcg gtgggctcgg tccaggagcc gggcaggcgg atgatctcct
    24121 cgaggctcgc atccggcccg gcgaggcccg ccagctccac gagcaacgcg tcgatgtcgg
    24181 tgcccggcag cagccgcatg tcgaggccga gccgcgcccg gtccggcacg gaattgtagt
    24241 tcgccccggc gcgcatgatg gcgacattga cggtcgaccg gccgagcagc ggatgacgcg
    24301 ccccgccgaa atcgtaggcg gcgaagcgct cggccgcgag gcaggcggca aggagcgcgt
    24361 tcctgccctt ctcgggctcg ctcgcatggg cggtcttgcc cttgaaatgc acatccagcc
    24421 agaggttgcc gcgattgccg aaggccgggc ggttggcggt cggctcggcc accacgaggg
    24481 ccgcggcacg gccgagagcg cccggccggt cggccagcac cttggcccct tcgagcccga
    24541 cctcctcggc ggaggtcagg acgatctcga ccggcgcctc gccgccctcc cgggcatggc
    24601 ggagcgctgc cgcgacgatg gcggcgacgc cgctcttcat gtcgctggca ccccggccgt
    24661 agagccggcc ggcctcgatc tcgccggcga agggctggcg ggtccagggc gcctcgccga
    24721 gcggcacgac gtcgagatgg ccggtgaagg cgatggcggg agcaccgctg cccaaggggt
    24781 ccagccgggc gacgaggttg ggccggcccg gcgccaagtc gacgccatgg cacgaaaagc
    24841 cggcgttctc gagcagcatc tgcagggcct ggatggactg cgcctcgttg ccttccggca
    24901 cgcgggtatc ccgggcgatc agctcgatgg tcagcttcac gggatcgatc atcgctttcc
    24961 cagcatcaaa gagccagcag ctcgtcgacc agcgccggca cggtggtgcc cgccgggcca
    25021 tggcgcgcct cggcgaagtc cgagcgcacg gcggatggct cgagattgag ctcgatcgtg
    25081 tgcgcccggc cgcgatcgcg caccagggcg acgaagccgg cggccgggta gacgaggccc
    25141 gaggtgccga tggcgaggaa gagggcgcat gactgcaggg cagccgtgat ccgctccatg
    25201 tggagcggca tctcgccgaa ccagacgatc gccggccgca gcccgccgaa agtgccacag
    25261 gccgggcaag gtgtcttggc ctcgaggtcg cgcagccaga ggcgctcctc gctgcaggcg
    25321 gtgcagcgca cgcgcctgag ctcgccatgc aggtgcacga cgtcgtggct gccggcccgc
    25381 tcgtgcaggt cgtcgacatt ctgcgtcacc agcaggcagc cgccccgcca tgcccgcgcc
    25441 agccgcgcca gggcccgatg cgccgcattg ggctggatgg cggggtcggc gagctggcgg
    25501 cggcgcatgt tgtagaagcg atgcaccagc gccggatcgc gagcgaacgc ctcgggggtc
    25561 gccacctcct cggcccggtg cccctcccat aggccgccgg cgccgcggaa cgtggcgagg
    25621 ccggattcgg ccgagatgcc cgccccggtc aggacgacga tggagccggt gcgcggcagc
    25681 ggcaaggctg ttctccagtg cgcttcaggg ctcattcagc atgctgccgg ggtcgccggc
    25741 aagctttgcg atatcggctc ttgtcaggca gcgggcaacg cgataccggc gcgtccgccg
    25801 cccgtatttc gtgcggcgtg ctggagtctc gtttcgatgg cggtccgtca accgatctgg
    25861 atccggcgcg tcgccgcccc cggggccgag gcgttcgccg tgctctacgc gctcgaatcc
    25921 ttctcgcgag ccatcctgac cagcatcctc ccgctgcagg cgctggccct gctcggcgag
    25981 ccgcagagcg tcagcctggt cttcttcctc ggcagcctcg ccgggctgtt cggcagtctc
    26041 ctgatcccct ggctcgtgcg ccgcaccgcc cggcgctatg tctactcggg cggcgccctg
    26101 ctgctcgccg ccgccgccct gctcctcgcg ttcgacggga tcctcgccct gacgctcggc
    26161 ctcgcctgcc gggtgctcgg cgtggtggcg ctgaccatcg gcctcagcct ctacatcatg
    26221 gatgtcatcg ccaggcacga cctcgcccat tccgagcccc tgcggatgct ctactcggcc
    26281 accgcctgga ccatcggccc tgccctcggc gtctggctgc agctccgttt cggggcatgg
    26341 ctgccactcc tcctgagtgc cgcaacctcc ctcacgctct tcgcctattt ctggttcctc
    26401 aggctcaccg aaacggcggt gctgaccacc ggccgcgcca atgcggcacc cggcgccctg
    26461 cagaacatcc gccgcttcgc cagccagccg cggctcgtcc tggcatggct gatcgccacc
    26521 gggcgcaatt gctggtggtc gatcttctac atcttcaccc cgatctatgc cgtgaccacc
    26581 gggctcggcg agatggcggg cgggctgctc gtctcgatcg gcaccggcac gcttttcctg
    26641 atgccgctct ggggtcgggt cgtgcgccgc ttcgggctca ggcgggtctt catcaccggc
    26701 ttcaccctga gcggggtcat gacgctggcg atcgccccga gcttcgccct gccctggctg
    26761 ggcgcgggct tcctcatggc cgcctcggtg gcgatggtgg tgctcgacgc ctgcggcaac
    26821 cggctcttct tcgccgcggt gcacggccgc gagcggggca acatgacggc cgtctacagc
    26881 acctaccgcg atgccgccga cctcgtcacc ccgggcacgc tcggcctcgt gctgcgcgtc
    26941 ttcgacctct ctgccgtctt cgtcatcggc gggttcctga tgttcggcgt cgccaccctc
    27001 gcccgccgca tccatccgag ggtcggcgcc gtgcgaccac cggcacccgc cctcacaggc
    27061 gttgacccgg cgccgcgcat ggccgatcac ccggcccaga ccgccggctg acgagccggc
    27121 ccatcagcgg gggagagagc gcatggccac catcggattc gtcgggctcg ggcatatggg
    27181 gggggccatg agccgcaacc tgatgcgcgc cgggcaccag ctcgaggtct tcgacatcgc
    27241 cgaggacgcg gtcgcggcac tcgtcgccga cggggccagg ggtgccgcga gcccggcggc
    27301 cgtggcgaag ggcaaggaga tcgtcttcac catgctgccg atcggcaaga tcgtcgagga
    27361 ggcggtcttc ggcccgaacg gcatcgccga gacgatcgcc gagggcagcg tcgtcgtcga
    27421 catgagcacc atcctgccga ccgagacgaa gcggatcggc gagcggctga aggcgcaggg
    27481 tgtcggcatg gtcgatgccc cggtcggccg cacctcggcc catgccgtca ccggcacctc
    27541 caccttcatg gtgggcggcg agcccgccga catcgagcgc gtccgcccac tgctcatggc
    27601 catgggcgag atggtgacga tctgcggccc gctcggcacc ggtgcggcca tgaagctggt
    27661 caacaactac atctcggccg tcgtcaacct cgccaccgcc gagggcctcg ccatgggcct
    27721 caaggccggg ctcgagctgc cggtgatggc cgaggtcctc tcgcacaccc cggcgggccg
    27781 cggccacatc accacgacct ggcccgagaa ggcgctgaag gacgagccct cgcccgcctt
    27841 catgctcgac ctcgcgtcga aggacctgag cctcgccctc gacatggcgg ctgccctgca
    27901 ggtgacgttg gccaccggtg cggtcggcaa gcagctctac gcctccgccc aggccaacgg
    27961 ccacggccgc agcgactgga ccaccggcat gttccggacg ctgaagaaga tgggtggcgt
    28021 cgccagccgt tgatggcctc gacgtggtga ccgacggcga gtggcgccgc gtttcctgta
    28081 tcggcgccat cgcggcgctg gcgcacggct tctcccgcct gagcggcggc aggcaggcgc
    28141 gggtgaagcc ccctccgtcc ggataggcag cagccgagcg cccctccggc cacagccgct
    28201 cgccgagcag cgccggggcc cggatcggac acgcggccgc cgaaacccgc aaccgccgcc
    28261 cgaccccacg ctcaccccgg cgctcgccag ctccagcgtt gccacaccca tcgccccacc
    28321 atgacggcga tcagaaagac accccagagt gctgtcgcca gatgctggtg ggcgcccatc
    28381 aggttgagcc cggaggcgat ggtctgcagg atcacgaggg ccagcaccac ggcggccgtc
    28441 cggccgaacc cgccgaaggg gtcgacaccg ccgagaaagc aggcgagcac ggtgatcagc
    28501 aggtatgcct cgccgtggcc gacccggacc gagttgaagc gggtcagcat gaggatgccg
    28561 gcgaccgcac tcatcacgcc cgacagcacg tagacgagga ccagcacccg ccgcgtgtcg
    28621 atgccggagt agcgcgtcgc ctccaggttg gagccggcca tgtaggtgga gaagccgaag
    28681 cgcgagcggc cgagcagcac ctgccagagg agcgccgcca cgagcaggat caagagcggc
    28741 aacgggatgc cgaagaccgt gccgtggccg atggtggcga tgaagggcgg aaagcccgag
    28801 atgtcgccgc cccgggtcag gaactcgccg agaccgcgca ggaagatcat catcgccagc
    28861 gagacgagga tcggatgcgc gccgacgaag gccaccacca cgcccatcac cccgccggcg
    28921 agcgccccta cggccagcgc cgccgccgtg ccgagcagca ccatcatcag cccagcctcg
    28981 ggcccgccgc cgagctggat gacccaggcc atggcgagcc cggcgatgtt ggcggtgaag
    29041 gtgatcgcga ggttgagccc gcccgacagg atcggcagca gcatggccag cgtgaggaga
    29101 cccagctccg gcagctggaa ggcgatcgac tggaaggtgc cggcggcgag gaaacgcggc
    29161 gtcgtcaggc cgaagaagac gagcacgacg gcgaagaaga cgagcagtcc caggatctcg
    29221 ccctggccgc gcagcaaccg ggtgaaggcg gcccgcatca gccttgccgc cccgcctcag
    29281 cccgggctcg acccgaccgg aagcgccgga tgaacgcctc gaggccctcc aggttgctca
    29341 tggtgatggc gacgagaatg acgaggccga cgaccatctg gaaggcgtag gggctcacgc
    29401 ccatgaggtt cagcccgttc tgggtcatgg tgaccagggt gacgccgagc agtgccccca
    29461 ggatcgtccc ccgcccgccg ccgagccgcg caccgcccag caccacggcc gccagcacgt
    29521 cgagctcgcg gccgtagagc gcgttcggca ccacctcctg gacgtagtgc gcctgcatca
    29581 gcccgccgat ccccgcgcag acgccgagcc agccataggc caggaaatgc atggcggcga
    29641 tgttgatgcc gacccgccgc gccccttccg cattgtcgcc catggcgtag agctggcggc
    29701 cgaggctcga gcggttgaga aagagccagg tggcgacgac gaccgccatc atcaccacca
    29761 ccggcagggt caggaacgcg aaatctcccc ccggcgtctc gaactcgaag acgatgatcc
    29821 ggtccaggag ccagtccggc agatcgtaga tcgaccggcc acgggtgaag aacatcagga
    29881 ggccgaagaa gatgttgaag gtggcgatgg tcaccacgat cgagatgatc cggaaatagt
    29941 agatcaggga ggcgttaatg gccccgagca tgaagccgaa ggcgctggcg aggacgagac
    30001 cgatcgccca gttgccgccg ccgagctgca tgaccgtcac cgcggtaagg tactggacga
    30061 cggacgcggc gaccgcgaac gagatgtcga tgccgcccgc caccagcacg accaggaggc
    30121 cgaccgccat gatcgcgttg aaggcgctga tgttcagaag atcgaagagg ttggcgaagg
    30181 tcaaaaacgt gtcggtggcg agcccgagaa cgccaccgaa aacgaggatg acgagggtga
    30241 gccacccctc ggtcgagcgc ggcagccagc gccgcccggc tccgggcatt cctggctcag
    30301 gcatggacgg ccgcctcgat cgcctcgatc ggcgcatggc ccggctggaa ctcgccaatg
    30361 agccgtccgg cgcgcatgtg caggatccgg tcggcattgt ggtagagctc ggggatctcg
    30421 tcggagatca tcaggatggc gaggccgctc gccgccagct cgcggacgat ctggaagatc
    30481 cccgcccggg cgccgatgtc gaccccgacc gtgggcgaat cgaggatcag caggcgcggc
    30541 tcggtggcca gccacttggc cagcaccacc cgctgctggt tgccgccgga gagcgtggcc
    30601 acggcatcct ccggccggcc gatcctgatc ttgagccggt cgacccagcc ggccaccagc
    30661 cggcgctcct tttccggatc gagcaggccg aggaggccga gcaggcggtc gagcacggtg
    30721 atcgcgatgt tgcgcgagat cgactgcggc tggacgaggc cgagcgaaag gcgatcctcc
    30781 gagacatagg cgatgccgga acggatcgcg tcgcggttcg agcccaggcg aagctcccgg
    30841 ccgtcgaggc ggatatcacc ccgatccggc gacgtcagcc cgaagagcgt cgccgccacc
    30901 tcggtccgcc cggcgccgag cagcccggtg atgcccagga tctcgcccgc gtgcaggctg
    30961 aagctgacgt cctcgtactc gccggcccgg gccagaccct cgacctccag gacgacacgc
    31021 cccgcgctcc ggtcctgcgc cctgaccacc gcctcgtgcg cctcgccggt catcagctcg
    31081 gcgagccggt tctgggtcag ctcggccgcc ggatagacgc cgaccatgcg cccgtccctg
    31141 agcaccgtca cccgctcggc gatctccagc acctcggcca ggcgatggct gacgaagacc
    31201 gtggcgatgc catcggcctt cagccggtgc accacctcga agagcgcccg ggtctcggcc
    31261 tgggtgagcg aggccgtcgg ctcgtccatg aagacgaggc gcgcctcggc gacgagcgcg
    31321 cgggcgatcg ccaccacctg gcgctgggcg atgccgagct cgcgcaaggg ccggccggtc
    31381 ggcagcgtca ccccgaggcg ttcgagcacc tgtgccgccg tccggcgcat cgccccgtag
    31441 tcgacccagc gcggccggcc gccgagattg gcctcgaacg cgatgttctc ggcgaccgtc
    31501 atctccggaa agagggcgag atcctgccag atcacctgga tgccgagccg ccgggcggtc
    31561 accggcgtca gcccctcgac ccgctcgccg gcgaagcgca gcacggcgcc gggctccggc
    31621 gcatgcacgc cgctgacgat cttgatcagc gtgctcttgc cgcagccgtt ctcgccggcg
    31681 aggcagtgca cctcgccctc gagcacgtcg aaatcgacgt cgacgagcgc ctgcacgccg
    31741 ccgaagacct tggagacgtg ctcgaccgcc aggatgcgcg gggcggcgtc ggtggcgccg
    31801 ccccgcaccg cctcaaagcc ccatgtcggc aagctcgtcg accgtgtcct tgttcagggt
    31861 cacgagcgcg tcgacgatga tgttgtgcgt ctcggcgtcc ggcgagacca ccccgagacc
    31921 gggaatctcg gtgccgtcct cgatcggctc acccttcttc agcatgtcgg cgatggtgac
    31981 gaagacccgg ccggcctcca tcgggttcca catgaagccg ccgctgatca ccccgtcatg
    32041 caccagccgc tgcccctggc ccggcgagaa cggcccgacc acggcgatct cgccgccctt
    32101 gcgccgctcg tcgacggccc ggcccgcccc gatcggcccc tggctgccga aggcgaggaa
    32161 gcccttgagc tcgggatgcg cgcgcatcag gtcgagggcg gtgctgcgtg aatcgtcgac
    32221 gctctcggcg accccgaagc ggtcggcgac cagcgtcatg ttcggataat tggcctccag
    32281 ataggccacc gccgcgtcgg cccaggcatt gtgcgccggc acggtgagcg agcccacata
    32341 gagcgcgtac tcgccctcgc cgcccgtggc ctcggcgaac agcttggcgt gcgcctcgcc
    32401 gaagccctcg agcgaggcca tctcgaagtt ccagtcgacg ttctgctggt tcggactctc
    32461 atgggtgatc acgatgatcc cctgctcgcg cgcccgggcc aggaccggct cgagcacctc
    32521 ggcatcgttc ggcaccacgc cgatcgcgtc caccccctgg gcgatcaggt cctcgacggc
    32581 gcgcacctgg agggccgggt cggcgctggt ggggcccacc atccaggcat cccagcccag
    32641 ctccttgccc tgctcctgga tgccgacctc catggcgttg aaccagggaa tgccgccgat
    32701 cttcaccacc acgcccatgc gcggcatgtc ctgcgccatc gcggtcgaag ccatcaggcc
    32761 catccccagg gcagccgcca ggccaccgaa cgtccgtcgc tgcatcttca gcctcccatg
    32821 gcaccttgcc ggccgagcct gttgcccggc ccatgcgcgg acgttagcgc aatggcgccg
    32881 cggctcaagt ggcttgcacc gcgagagccg gcagccgggc cggatcgaag ggggcgagat
    32941 cgaccgtggc cgacgccccg tccatgatca gcgccgccag cgcctcgccg gtcgccggcc
    33001 cgttcagcat gccccagacg ctgtggccgg tggccacgaa ggcgccctcg agcccggcga
    33061 ccggaccgat cagcggcagg ccatcctcgg tcaccggccg aaagcaggcg ccgccgtgcc
    33121 gcaccggggc ggcggcgagc cccggcgcga agccccggat catctgccgc aactgctcga
    33181 ggaagcgctc gtccgccacc accgccgccg gatcctcggg cagcggccgc cggctcggca
    33241 ggccgcagac ataggtggtg ccgtccggcc ggggaatgac ctcgggcgcc ccctgcccgc
    33301 cacccggtgc cgggcaatcg acgaagagcg cggccgccgc ggcccccggg tcgtcgaaga
    33361 cgaggctgtg cccctcgagc ccgtggaccg ccggcagcgc cagccaggca gccgccagga
    33421 ccgaccacgg ccccatggcg atgaccacag c
//
LOCUS       DAOTFI010000012        32885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1497_length_32885_cov_6.567408, whole genome shotgun sequence.
ACCESSION   DAOTFI010000012 DAOTFI010000000
VERSION     DAOTFI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 32885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32885
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1497_length_32885_cov_6.567408"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="P5558_02060"
     CDS             complement(<1..1459)
                     /locus_tag="P5558_02060"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY23126.1"
                     /translation="MTDAALSESTAAASRGAAARLWRVLDLALEAVAALLMLTAAGIA
                     CLQVFFRYVLNASLAWPEEVALWAFGWAVFIGMAVALGRGGHIAIDLASRLMPAAWRP
                     AHRFFVTTMIAAASVMLIVHGIDFLSRAMQVSPAMQWPLKYLFLAVPVGGALNLCYLA
                     RPREGAAWFGLAAILAGGLVYLAVRYGAGLVYGEAGSAAVLMIVGLALIMLEVPIAFA
                     LAFGAFAAFAPLGNMMLVIVTQSMSSSLNSFTLLAIPFFIMAAALMNAGGITTKLVDL
                     ATHLVGHLRGGLGQANVVTNTLLGGVSGSSSADASAITKLLVPEMERRGYARAFSCAL
                     TSAASTLANLIPPGLGLIIYAALASVSVGALFVATIVPGLMAATALAVVVYLVSRARG
                     YGADVPRSTGHQRLIALRNAVPALLLPLVIVGGVRFGVFTATEAGAIAFVFAIFCGAL
                     IYRRLTVANFTSSIREAVFDTVAIAVIIAAAAPFAW"
     gene            complement(1456..2499)
                     /locus_tag="P5558_02065"
     CDS             complement(1456..2499)
                     /locus_tag="P5558_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY23127.1"
                     /translation="MTAGSNTRTALGRRRFVATGAALGSAAIAMPAVIRPAHAAEFTC
                     RIAHSEAIGSPLTNAFEAWKDTLNVESGGRIDAQHFPASQLGGLAQLLEASRIGTIQV
                     TTAGPDSEEAIAPEIAAFGGAPGFIYKDEAHVDRVLQGPLGQEVSDIARAKTGVEFVA
                     YGETGFRHLLTKSPVTSLDTLSGVKLRVPELRIWVDFWTKLGANPTPLPYSEQYAALS
                     TGIIDGLDSDFFSIIGFKWHEQATNLTLTYHWFLPKAVRVNAAWLDSLPPDLQELVRT
                     SAKEAFAAQRAENRAKADQALVDLEGVGVTVYRIDDAERAKWAELTSPLFEEFGSKSP
                     ETQAMIDKIRALG"
     gene            2722..3810
                     /locus_tag="P5558_02070"
     CDS             2722..3810
                     /locus_tag="P5558_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY23128.1"
                     /translation="MAAVALGLLFGADAALAQSPPLKIPFVDEWAASPHARLGAEAFN
                     HWNAEGEVPVPCARCHSTPGFLDYIGADGTPPGLDGPAPVGTVISCVACHNDVTIRMD
                     SVTFPSGAVVDKVGDDARCMTCHQGRESTVSVDAQLAGAEPDTVSDELRFINVHYRAA
                     GATRYGTEARGAYEYPGLTYVGYFLHDPDSQRCADCHQQHTVRVMVQACATCHQAPQL
                     TRLADLVKIRKNSALDFDGDGNVEEGIAEEITTLHAALYQAIQGYALAIGGQAIVYSA
                     GTYPYFFNDSNANGKVDPGEAVFPNAFKSWTPRLLRAAYNYQYVVEDPGAYTHNPLYV
                     IQAMHDSLQDLGTRVEVDMAGMARPEAE"
     gene            3878..4780
                     /locus_tag="P5558_02075"
     CDS             3878..4780
                     /locus_tag="P5558_02075"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRY23129.1"
                     /translation="MAAFELASDSLALPPGAWARTHRRRLRHDGRTVLALTQGAFRPY
                     VFPLMTPAGFAVTAEAPADHPHHASLWLGADHVHALTPTAPGAVEEYTYNFYTDETFQ
                     GRAPGRLIERGCEGAADGDAFVIDQAIDWQGPEEWAAAAGRLVARERRVTRVALGPGH
                     YRIDVTSTLTAGERAIRLGPTRHAFFNVRVADGLMVANGGRVQDDRGRAGGAAISGEG
                     ARWVDFSGPVGGGEWAGVAVIAEPIGDRAPSWFVADWGVITVGPFRSKALLLEPGQDF
                     TAHFAVIVHDGDAEAAGIGRLAGD"
     gene            4804..5574
                     /locus_tag="P5558_02080"
     CDS             4804..5574
                     /locus_tag="P5558_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY23130.1"
                     /translation="MHIARYLYEGEVRWAVVEPDGLHRLAGAPYAGIERTGAVDALHA
                     ARLLCPIEAPRIFAIGLNYVDHIKESGSRTPEIPLVFMKPSTAAIGPGEPIVYPLEGQ
                     EVHFEAELAVVIGRRGRRIERDRALDHVLGYTCANDVSERVIQKAEMAQGALVLCKGF
                     DSFCPLGPTIATGLDPRDLKIGARVNGEVRQASSTSDLLFSVADLVAYFSTALTLLPG
                     DVILTGTPSGVGPVKPGDVVEIEVEGVGVLKNPVVAEA"
     gene            complement(5595..6521)
                     /locus_tag="P5558_02085"
     CDS             complement(5595..6521)
                     /locus_tag="P5558_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY23131.1"
                     /translation="MKGGRVLLELWIPITIFAAFCQNLRSALQKHLKGRLGTTGATFV
                     RFGYGFPFALLYLAVLHAGLGHKLPEPNVTFFAAGALGGLAQIIATFLLVHLFSYRNF
                     AVGTAYSKTEPVQAAIFGLVLLGETVTAGAVAAILIGVAGVVLISIARQPLTLRSVIS
                     SLSGRPALIGLASAASFGVSAVLYRQASTALAGPSFIMQAAFTLAFVTLFQTFVMVLW
                     MWRREPDQLRASLVNWRVAIWVGLVGVAGSAGWFTAMTLQKVAYVRALGQIELVFTFL
                     ASWLVFKEAINKSELLGCIVIVGGILLLLVAR"
     gene            complement(6564..7505)
                     /locus_tag="P5558_02090"
     CDS             complement(6564..7505)
                     /locus_tag="P5558_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HRY23132.1"
                     /translation="MSERNFGEAETDAERAVEAAIARVPAWREQGASYALMTGGISNS
                     NWRITLAGDERRYFLKIPGAGTEMFVDRVAANEAARLAHAKGLGPEVVHFDAETGLEV
                     AELLEGYRACTNADFQDEAIQLAAVDCYRQFHAGEGLGLTKTIFDMIDEHIAQVAGLG
                     GSLPEDWPWLSRLYGNARSAFLASGLDLVPCFNDPMPGNFLVKPDAPMKLVDYEFASM
                     NERSYELGVWLGEMFFTEDTAARLLEAYAGELRPELVARVAVMRALADVKWATWAMVQ
                     EQISALDFDYHKYGTWKYLRARSIMHDFRWEAWLRAL"
     gene            complement(7507..8457)
                     /locus_tag="P5558_02095"
     CDS             complement(7507..8457)
                     /locus_tag="P5558_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRY23133.1"
                     /translation="MSTRDALEAARALVATVPGWAGREPTLRPGVAPVASPMNQGVDA
                     TSLRARDAATGESLWVKIADPDAALFADPASVVAAAREAGTLGVGPEVVAADPLTGDL
                     VMRDLAGSHRTATLNRLAEPAIRDAVLAAMRAFQAGPPLPRTMGVFDHIESLLRRAGA
                     AGVALPADHAWLLDNIRTAAQAIRAAGIDTVPAHGDGNASNVMIDEAGGVLLVDFDLA
                     ANRDPFEDLGSFLVEANTFDPAARESFEAFHGRFDERLFNRSRLYGVADDLRWGLIGA
                     ILARTSARTDLEFLKYAEWRFLRCRMAMRDQRFEERVRRV"
     gene            complement(8454..9722)
                     /locus_tag="P5558_02100"
     CDS             complement(8454..9722)
                     /locus_tag="P5558_02100"
                     /inference="COORDINATES: protein motif:HMM:NF006772.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRY23134.1"
                     /translation="MTRPDHIHRELHEQIARFSTFGATKAGGIHRPEATAANGEARDA
                     LVAWLRESGFEVRIDAIGNIFGLAGIAGPEAPWLLTGSHVDSQPNGGRLDGTYGVIAS
                     VIAARELRQRLAAPAMNLAVVAWTNEEGARFQPSVLGSQAYVGAIDTDWALAREDRDG
                     ITVRAALEAIGHLGTDQPAPRPAAYVELHIECGPLLERAGRRLAAFDRWWGCRKLEIR
                     FEGVPAHTGPTPMAERKDALLAAAGVITGLRQLVDMAPPGELHTSVGRIEALPNSPNV
                     VPSTVTIFAELRSADPAVLETAFARTEALIEAEATRAGVGWSFARDELRRPGRFAPGL
                     QRLANEVATGLGEPAMRLDTIAAHDAVPMARLCPSIVVATPSIGGICHSPLEDTRPED
                     LALGLDLLTGMLERLLVEGPDLAKAGETAS"
     gene            9905..11509
                     /locus_tag="P5558_02105"
     CDS             9905..11509
                     /locus_tag="P5558_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23135.1"
                     /translation="MSSLLMPGKRRFRTNRRRVLGGMAAAGGLAAFPAPYIKRAWGQA
                     RNHMVFASAEPVTGNWDPTSHTTLAQINLEGFVFGKLFQTPMRPENPDELVFELATSQ
                     EVLDLHTIEYKLRDGVTFHNGAPFGAEDVKATFEYASQPGRPAAWYPGPVEVEVVDRL
                     TARVHTEIGDFPASAFYFLAGFLPIMSKDDVADPTVLQERPNGTGPFKFVEQRGNTTV
                     LAAFDGFMHGRPILDEVHFAYVGDATTRVLALLSGEVDVVERLEPEQYETLAGESGVA
                     LSRTISTENKYLHFRCNKAPFDDPRLRMAACHAIDRELVTEILGVAGHASSNYISPVK
                     FGYIDLPNYPSYDPARCQELLAEAGFPGGQGLPTLEYLTSDGFYPKTKEYGEVITALL
                     QEQGFPVELTVMETAAWLERIYQREGQEPYGHLVDVGWSTGSPEPDLVLRPMFHSSAG
                     PRGGLINGIADPAIDAVLDQQQAETDTAKRRELIQTVATPKLAELVPSFSLFTSVLLH
                     GRRAGLEGLHFYPNGPIDASKAEYKA"
     gene            11528..12505
                     /locus_tag="P5558_02110"
     CDS             11528..12505
                     /locus_tag="P5558_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23136.1"
                     /translation="MGFALTFFARRLGQGVLIVLLVSFTIFALLRVVPGDPARIILGP
                     MATDEVVEQTARDMGLRDPIPLQYMRYLGQVAKGDFGTSFIKAKGGGSMGGGRDEARG
                     ERARVLDLVAETLPLTLQLAGLALVFTALIALPLGIAGGLMAGRWPDKLALYASSIFV
                     SLPNFWLAIVLALVVTAKLGWIPSIGYQNIAYAILPAFVIAIEMSPLFIRSLSVSIAD
                     TMRHPFVELAQVRGLSPRRIFSHHVLRNAAVPVLNLFGVQIGALLGGVLVVEYIFDYP
                     GFGLLTVQAVLQRDFPVIQGIAIISTSIFVVINILVDFVSAQIDPRIEY"
     gene            12505..13419
                     /locus_tag="P5558_02115"
     CDS             12505..13419
                     /locus_tag="P5558_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23137.1"
                     /translation="MAASIDEPAAIAATAVAATPGRRMWLATWSQTEFKIGLVLFLLL
                     VGTAFIYPLLSDIDPTKLSVRERFLPPVFLEGGTWAHPLGTDQLGRDLFVRSLVGLQN
                     ALMISVAVVVLMFAFGATVGLVAGYRGGWIDTILMRLTDAQLSIPLIILAIIILTVSR
                     PTPFSVIVVLALAGWPVYARVTRSVTLAERRREYVRGAKILGASDSRIMWLLIAPNIL
                     PPIAFVAVLDIARMMIFEAILGFIGIGIQPPTPTFGTIIADGRKYLINAWWIATMPGG
                     FLFLTLLSVNLMGAALERARNRLLRGTI"
     gene            13423..15240
                     /locus_tag="P5558_02120"
     CDS             13423..15240
                     /locus_tag="P5558_02120"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23138.1"
                     /translation="MSERLLTVEGLSVDLVRDGGLVPVLRDVSLALEGRQVLGIVGES
                     GSGKTVLAKALVGWVAEPLVASRGVVRFRGQDLLAMPPEARRILRGSAVGYIGADPGS
                     SFDPTLPVGRQIVEKVQAVRADIDARAAKKRVLDLLDRVHIPSAARRFDEFPHQYSGG
                     MLQRAMIVDALASDPAFLVCDNITQPLDVTVAAQIMRLLHELRSDLDAGIVFISTSLP
                     VVCDIADELVVLSRGEIVERSTPARLLEAPAHPYSQDLIARNPIIWGTPPLPVPAEAS
                     PRVILAVDDVTKTYVVPDRTRFFGTQAVKAVRGVSFAVHGGENLGLVGESGCGKSTLS
                     RLLSWVEQPDQGRIRFKGQDIAGMSAQQLLAMRKGFQLLLQDPFNCLPPNLPIGRSVA
                     MSLQIHGGLGRKAIAEQVRAVMAEVGLKEDDRERLPIGMSAGQRQRVNIARALILEPE
                     LLILDETLSALDPMEQARLLDLFERLQARHGFTYLFISHDLAMVRRVCTRIAVMYLGK
                     VVELADTRSVFFEPTHPYTRALLSAVPTLEEKPFKAADCLLEGEPPSPIDLPQGCSFA
                     ARCPHAMPRCRVEEPPLVDLGGGRFSACWLNEPEVSRAA"
     gene            15253..16872
                     /locus_tag="P5558_02125"
     CDS             15253..16872
                     /locus_tag="P5558_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY23139.1"
                     /translation="MASDESGMWDFIVVGAGGAGAVLAARLSEDPATRVLLLEAGPDY
                     RSAEQPPEMASPNPFNIILPEAFQATYMWPALLARRTKRQAHRLYWRGRGVGGSTAIN
                     GQIAIRGVLDAFDNWAEHGCEGWSAADVLPGFNRLETDEDFAGEAYHGGSGPIPVHRT
                     PPDAWGPVDRALRDAALDLGYPWTPDLNAPTAEGVTTYAINSQGGRRVSTNDGYLEPA
                     RGRPNLEIKGGMLADRVLMDGRRATGIAAIGPEGLATFDLAPGGEVLLAAGAIHSPPI
                     LLRTGIGPAAHLAEHGIAVIRDLPVGDGLFDHPFVRLELKLREELKPTSVDARHTNCC
                     VKYSSNLPGGSLADMIFFAMNHGGVGVAQDMAQFGEAGIHCALFEAHSRGQVRLASTD
                     PRTQPVVDLNMLDDERDLARLRDGARRLMEIGAHEAVTGKCREVSLGNTGRPIADLVE
                     APDEAVDDWILADCSDAQHGAGSCRMGAFDYEDGRSVLDPNCRVRGIEALRVIDASIM
                     PADCKANTAFTTMMIGEQMAERLRRQRAGGG"
     gene            16881..17651
                     /locus_tag="P5558_02130"
     CDS             16881..17651
                     /locus_tag="P5558_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRY23140.1"
                     /translation="MSELARHATILAALGRDGRVGVAALAQALGVSDETIRRDLATLD
                     AAGRLRRVHGGAVQFRPDEEQPILVRSRIRPREKAQLAAVARGLVKDGMSIFIDTGST
                     ALAFARTLADARDLAVITNSLDIARLLAPAPGLRVKVAPGWVRANDDALVGGDTVAFV
                     RRFVFDIAFMGIAACDADYGWMDYADEESELRRAVIEQTRCPVLLVDDSKFGRHASIR
                     TFDLTTPLTVVTNRPVPAPFAEIFASAGVEVRHRLRGG"
     gene            complement(17648..18967)
                     /locus_tag="P5558_02135"
     CDS             complement(17648..18967)
                     /locus_tag="P5558_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRY23141.1"
                     /translation="MPTDPDLLSLNAFDPARGAAADPGTAALLARRQANLGPVSVLFY
                     EEPLHIVRAEDVWLEARDGRRYLDVYNNVASVGHCHPKVVAAIAAQAATLNVHTRYLN
                     EPTEAYLERLKATLPAALSNLALTCSGSEANDLALRLARTATGRQGIIVTAAAYHGNT
                     SAVSEISPSAFKQGRPPAHVRIVPAPDARHYGTDLAGGFAASVWQAIGELDEAGFGCA
                     AFIADSIFSSDGVHADPPGFLQAAAAVVRSAGALYIADEVQPGFARTGDAFWGFERHG
                     IAPDIVTMGKPMGNGFPMAGLAARPEVMAPYFAEFGYFNTFGGNPVAAAAGLAVLDVL
                     REQGLQQNAAETGRYLRRKLEALQASHPSIGTVRGAGLFLGVALCRDDDPDPGAARRV
                     INGLRQRGVLIGACGRHGEVLKIRPPLTFKPEHADELVDALAEALTD"
     gene            19137..20726
                     /locus_tag="P5558_02140"
     CDS             19137..20726
                     /locus_tag="P5558_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23142.1"
                     /translation="MFRHIRNGIVGSTALALVSAAALAATPPDMLVIGTDVGAIPTLD
                     PAALNARTVSELVSNLYDNLLKLDPDDLQSIQPMLALSWEVAPDSSSITLKLRDDATF
                     ASGNPVTAEDAAWTIQRVIKLGQVGATDLAQWGFTADNVEQLVRAEDERTLVIELPEQ
                     VSTDLVLYSLAGSSLGIIDRKAALEHETDGDLARDWLRANAAGSGPFTLVEWRPDDIV
                     VTERREDYWGGTPAMRRVIMRHVPESGNLRLQLEAGDVDIGQYVGSGDLEALAGNEDM
                     EIQHTPGFGFYYIALNQKDPDLSKPKVREAFQHVLDWEALSQSSMRFNGFPWQSIIPR
                     GMAGAPEEFEEHFAYDPERARELLAEAGYPDGLKKKLYPAGDGHLLNAESLQATARLA
                     GIELELIPGNHVPEFRARDFEVYTGNSGGRLPDPFATATHYAFNPDNSDEAQLSGYYM
                     WRTAWDVPELTELTNESKRESDPEKRAQIFREMDEMYQQLDPSLIVFFQRVDPYVVRA
                     EVEGYVGHPTWSTRWADVTKN"
     gene            20748..21797
                     /locus_tag="P5558_02145"
     CDS             20748..21797
                     /locus_tag="P5558_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23143.1"
                     /translation="MPSVSILPGPRARRARLGRLAGRTVSLALTLLGLMALTFLMGRL
                     LPADPVLAVTGFEVDRATYDRVFLELGLDRPVWQQFLAYVGGIFQGDLGRSTTTGQPV
                     AVDLLRVFPATFELALTAALVGSLLGIPLGIVAAVNRDRMIDHIARIIGLAGYSIPIF
                     WMGLMALLVFYAKLRWVGASGRVDVYYEGLVPSRTGLLLVDSAIAGDWAVFRSAFAHL
                     ILPASILGYYSTAYISRMARSFMLEQLGQEYIVAARAKGLPRHAVVWRHAFKNIRVQL
                     LTVVALVFAGLLDGTVLVETVFGWPGVGQYLTRGLQYNDMNVVMGTVLVIGLLFLAMN
                     TITDWLYRRLDPRTR"
     gene            21794..>22272
                     /locus_tag="P5558_02150"
     CDS             21794..>22272
                     /locus_tag="P5558_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HRY23144.1"
                     /translation="MSGTARPGSDSLGRPQGLRAWLDAEEPRSLRQARAQRAWRGWQA
                     FLGHRLGVVGLVLVVGLVLVALLAPWLATYDPLIQVMDERLQPPSAAHWLGTDHLGRD
                     IYSRIVWGARTTLRIAVLVAVIVAPIGLAIGATAGYLGGIADEILMRVTDIFLAFPGL
                     "
     assembly_gap    22273..22282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22283..22728
                     /locus_tag="P5558_02155"
                     /pseudo
     CDS             22283..22728
                     /locus_tag="P5558_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012686.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            22821..23489
                     /locus_tag="P5558_02160"
     CDS             22821..23489
                     /locus_tag="P5558_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY23145.1"
                     /translation="MRRNAEISRARLVEAATEEFAARGYDGARVDGIVARAEVSKNLV
                     YHYFAGKEALFIAVMERVYVLMRAHHQDDLLKQLEPRAAIERLIRSVHRLFVERPEII
                     TLLNSENLHKAAHIAKSGTIRTLYNPLLEALIDILDRGSAAGVFRRGVDPVDLYITIS
                     GLSYFYISNAHTLTFIFNEDLLAEERLARREQHIVEVVLGYLEAGRAKDAVNRRPARA
                     ALPG"
     gene            complement(23452..24393)
                     /locus_tag="P5558_02165"
     CDS             complement(23452..24393)
                     /locus_tag="P5558_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY23146.1"
                     /translation="MTGGALLGGPLLGGLALLATATLWGSNHVVARGSTEIVPLAAFV
                     FWRWAAALPILIAVAWPGLCRDWPVLRERWRDLLLLGTIGVGLFSVLLIAGAYYSLAI
                     EVGIISSTTPAWVAVIVWLKGRERIAATTLAGLAVAFAGALLVVTRGDPAAVLGLGAG
                     IGNLYVLASAILFAWFTTRLRPYRDRIASLSLTTATATAGTLTVLLPTYLVWLAFGGE
                     PYVTTGADAATALLILAYAAIAPTLLANAFYIFGVSRLGPQRAAAFLYLTPIASAAMA
                     VAFLGEALAWYHALGFVLVMAGLLLVSRATPRAPDAG"
     gene            complement(24401..25204)
                     /locus_tag="P5558_02170"
     CDS             complement(24401..25204)
                     /locus_tag="P5558_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23147.1"
                     /translation="MTGWRKFLMGESRTRDVLLVLAALVAIWELGVWHYEPPRIFLPP
                     PSAILAEFLTTPELFIRHMGYTLWTTLLAFTISVALGITLSVAIVYSRLLERTIYTLL
                     VALNSVPKVALAPLFVIWLGTGMEPKIAVAVMLALFPIVIDTVLGLRSVDPDMVNLAR
                     AARASQLQILAKIRFPNALPSLFAGMKVAISFALVGAIVGEFVAGGNGLGFLVLVAQG
                     QFDTTRVFVSLVLLGLMGTALFYALDFAERLMLPWHVSQRGEGGSQHGG"
     gene            complement(25201..25986)
                     /locus_tag="P5558_02175"
     CDS             complement(25201..25986)
                     /locus_tag="P5558_02175"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23148.1"
                     /translation="MNQGTQPYVALDRVAKVYPSPRGDVEALADIDLEIAPGSFVSVV
                     GPSGCGKSTLMKCLAGLVPITSGTIDINGRSLEGPPGAMGIVFQRDVLLDWRTILDNV
                     LLQAELRRLPRADYRERAMQLLARFGLEGFEDRHPWELSGGMRQRAAICRALLCDPEL
                     LLMDEPFGALDAMTRDDLNVELTRIWQGTNKTVLFITHSIVESVYLADRVVMMSRGPG
                     RIVEVIDIDLPRPRPLSIRETPAFGAYVHQIRHHFAELGILKE"
     gene            complement(25993..26976)
                     /locus_tag="P5558_02180"
     CDS             complement(25993..26976)
                     /locus_tag="P5558_02180"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23149.1"
                     /translation="MAARRTILSSTLATALMAAATGLAAAQEPLTVRLDFSPWGVHAG
                     MHYAMNEGWFEEAGLAVDLQDGRGSGNTLQLVNAGQVDVGQIQLGLLGAAREQGAEIK
                     SFAGFQRKTDLCILVDRDAPIQKIADLKGKALVVFAASPWAPYIDEYLAAGGLDQTTV
                     EVLFVDPAALWGTYTAGRADGLMSTIGSAIPVAEKSRPSKCMMLDEAGLTFPSYGLIA
                     TEATIAERAEDLARLVAVEQRAWAAIRDDLDLGVEAIIAQRPDANLDPDILREQIRIT
                     LDYFDTPATAGKPIGWQAREDWEKALQGMEKAGLIGPGWNPDDYFTNDLVQ"
     gene            complement(26999..28747)
                     /locus_tag="P5558_02185"
     CDS             complement(26999..28747)
                     /locus_tag="P5558_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY23150.1"
                     /translation="MTKSDASPFADPITFAVIKNAFDTIVDEMAYNVMRTARSHIVRE
                     VLDYSTTLCDRQGRIVAQAKTVALHLGAVPDAIEAVQAAYGDDLHPGDVVIMNDPYEG
                     GMHLPDIFMFKPVFFDGALQGFTVVVAHHCDMGGRVPGSNASDSTEIFQEGFRVPPLK
                     LYERGEPNKTLFRLFEKNVRVPDLVIGDLLSQYATCRMGERQYLRLFERYGAATVESY
                     IEGLLDYAETMTRREIEAWPDGTYSFTDHIDGDGFSDEPIPIKATITVEGGHLGVDFE
                     GSAPQVKGAINSTLSFVKSASYLSVRCVMDSDAPNNAGVFRCIDVKAPLGSMLNPRLP
                     AAVAARALTGYRIVDTMLGALAQIVPAKVMAAGEGGNTVVCLGGYDKATGEPFILVDM
                     INGAWGGRVDQDGIEGVTNPAQNMSNLPVETLEARYPVRIEEFGYRQDSCGAGKWRGG
                     LGLTRTYRLLAPEAMLQLRSDRTAHAPYGLFGGEPGALTVNILNPDRNAEAVPGKLTR
                     NIEAGDSLCHHQAGGGGYGDPLERDPALVAIDALDEKISRDFARDRHGVVLSDNFMPD
                     TAATAALRAGMRAQRT"
     gene            complement(28744..30855)
                     /locus_tag="P5558_02190"
     CDS             complement(28744..30855)
                     /locus_tag="P5558_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRY23151.1"
                     /translation="MGLAEPGGRTVRIAADIGGTFTDLVLVKGAGEVLTAKVSSTGSA
                     PEAAVLDGIRAIMAAAGVDGSQVVEVLHGTTIGSNTLLERTGAKTGLITTRGFRDILE
                     IGRVRTPEMFDLAWTKPEPLVPRRARLEVTERIAADGTVLTPLAEAEVLEAGRRLQQQ
                     GITSVAVCFINSYRNAAHERRVGELLAEHFPAFEVTLSAALQPEAKEYERTSTAVVNA
                     YVLPVMRGYLARLAAGLAGLGIAAPLLVVSSSGGLARSAVAQEQPVLFISSGPAAGVT
                     GAARLGTAGKIGDLIVFDMGGTTAKAALIEGGRISRTNEYEFRAGISTPSRFIKAGGF
                     LMRAPTIDIAEVGAGAGSIARVDAGGLIHVGPRSAGAEPGPACYGKGGDSATVTDANV
                     VLGYLNPKELAGGTLAIDAGLSEQAITTHVAAPLGVTAAEAALGVREIANLNMARAIR
                     AVTVERGVDPRDFTLVAFGGSGPVHACDLAAMLGIRHVLLPNLAGVFTAAGMLAGDIE
                     RQLVEPLPAPLDEALGPRLQAALDRLAAEARAALAEEGFTADRMAIERQIDLRFENQD
                     TELGITVEGAIEAGFAAPLRERFLEAYERVYEYRARDRVEVVAVRVVGRGHRAGKLDF
                     TSYRTAAAGGTNVASERQVMFARESGYVSTPVIDRSALPEVAKGPLIVESLDTTAVIP
                     PGAVVRADEAGNLRIELEGRS"
     gene            complement(30932..31864)
                     /locus_tag="P5558_02195"
     CDS             complement(30932..31864)
                     /locus_tag="P5558_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRY23152.1"
                     /translation="MTHLARRRCLTILAASAAGLALPAGAGAAVPAQRWSGMALGAPA
                     ALVVRHDDAARSTRLIEAAVAEIRRLERIFSLFDPSSALARLNGTGRLDDPPFELVDL
                     LHQARAVHDATGGAFDPTVQPLWRRHAEAAAAGRAPDPEALRAARAAVGMEAVAVDTH
                     EIAFGRPGMALTLNGIAQGYVTDRIADLLRAGGLAHVLVDLGEIRATGERSPGEPWRI
                     GLGTAEEIVPLTDGAVATSAPFGTVMDAAGGIGHIIDPRLGRPSPGNWRRVSVLHPRA
                     TIADGLSTGFVLLDRTAIDRALEAFPPARAITTA"
     gene            complement(31861..32262)
                     /locus_tag="P5558_02200"
     CDS             complement(31861..32262)
                     /locus_tag="P5558_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23153.1"
                     /translation="MNTSSRLLKALAVVAVVFGIATVLSGGRALFGGPEARAAVGNAV
                     PFVLWFNTLAGFVYIAAGIGLWRARPWAPRLAWLIAIATLFVFVAFAVHVATGGAFEM
                     RTVGAMTLRSLVWLAIAWLGWSRLAAESRTP"
     gene            complement(32259..>32885)
                     /locus_tag="P5558_02205"
     CDS             complement(32259..>32885)
                     /locus_tag="P5558_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY23154.1"
                     /translation="LALLFLGRGVFCGWLCPFGALQELTNKLARLLRVPQLALPFGLH
                     EKLWPTKYIVFLGLLALSLHAMSAATLAAEVEPFKTAIILRFVRDWPFVVYALLLLGA
                     GLFIERFFCRYVCPLGAALAVPARLATFRWLKRRPQCGRECRICEVNCPVQAIHPSGE
                     IVPNECIYCLDCQEHYHDAHTCPPLIKSRKRREAAAALHAPAGGGSPT"
BASE COUNT         4977 a        11206 c        11839 g         4853 t
ORIGIN      
        1 cccaggcgaa gggtgcggca gcagctatga tgacggcgat ggcgaccgtg tcgaagacgg
       61 cctcgcggat cgacgaggtg aagttggcga cggtcaggcg gcgatagatc agcgcgccgc
      121 agaagatggc gaagacgaag gcgatggcgc cggcctcggt ggcggtgaag acgccgaagc
      181 ggacgccgcc gacgatgacc agcggcagga gcaaagccgg cacggcgttg cggagcgcta
      241 tcagccgctg gtggccggtc gagcggggga cgtcggcgcc gtagccacgc gcacgcgaca
      301 ccagatagac gacgaccgcc agcgccgttg cggccatgag gcccgggacg atggtggcga
      361 cgaagagggc gccgaccgag accgaggcga gcgcggcgta gatgatgagg ccgaggcccg
      421 gcgggatcag gttggcgagg gtcgaggccg cgctcgtgag ggcacagctg aaggcgcgcg
      481 catagccgcg tcgctccatc tcgggcacga gcagcttggt gatcgccgag gcgtcggccg
      541 aggaggagcc ggagaccccg ccgagcagcg tgttggtgac cacattggcc tggccgagcc
      601 cgccccggag atggccgacg aggtgggtgg cgagatcgac cagcttggtg gtgatgccgc
      661 cggcgttcat cagggcggcc gccatgatga agaaggggat ggcgaggagc gtgaaggaat
      721 tgagcgagga cgacatgctc tgggtgacga tcaccagcat catgttgccg agcggggcga
      781 aggcggcgaa ggcgccgaag gcgagggcga aggcgatcgg cacctcgagc atgatcagtg
      841 ccaggccgac gatcatcagg acggccgcgc tgcccgcctc gccatagacg aggccggcgc
      901 cgtagcggac ggcgagatag acgagcccgc cggcgaggat ggcggcgagg ccgaaccagg
      961 cggcgccttc acgtggccgg gcgaggtagc agaggttgag cgcaccgccg acgggcacgg
     1021 cgaggaagag atacttgagc ggccactgca tggccggcga gacctgcatc gcccggctga
     1081 ggaaatcgat tccgtggacg atcagcatga cgctggcggc ggcgatcatc gtggtcacga
     1141 agaagcggtg cgcggggcgc caggcggccg gcatgagacg ggacgcgagg tcgatggcga
     1201 tgtggccgcc cctgccgagg gcgaccgcca tgccgatgaa gaccgcccag ccgaaggccc
     1261 agagcgcgac ctcctcgggc caggcgaggg aggcgttcag cacgtagcga aagaagacct
     1321 gcaggcaggc gatgccggcg gcggtgagca tcaaaagggc ggcgacggcc tcgagggcga
     1381 ggtcgaggac acgccagaga cgggccgccg caccccggga tgcggcggcc gtgctttcgg
     1441 aaagagccgc gtcggtcacc cgagcgcgcg gatcttgtcg atcatcgcct gggtttcggg
     1501 gctcttcgag ccgaactcct cgaagagcgg cgaggtgagc tcggcccatt tcgcccgctc
     1561 ggcgtcatcg atccggtaga cggtgacacc gaccccctcg aggtcgacca gcgcctggtc
     1621 ggccttggcc cgattctccg cccgctgggc ggcgaaggcc tccttggccg aagtgcggac
     1681 cagctcctgc aggtcgggtg gcagcgagtc cagccaggcg gcgttgacgc ggacggcctt
     1741 gggcaggaac cagtgatagg tgagggtcag gttggtcgcc tgctcgtgcc acttgaagcc
     1801 tatgatcgag aagaagtcgg aatcgagccc gtcgatgatg ccggtcgaga gggctgcata
     1861 ctgctcgcta tagggcaaag gcgtcgggtt ggcgccgagc ttggtccaga aatcgaccca
     1921 gatgcgcagc tccggcacac gcagcttgac cccgctcagc gtgtccagcg aggtgacggg
     1981 gctcttggtc aggaggtggc gaaagccggt ctcgccatag gccacgaact cgaccccggt
     2041 cttcgcccgg gcgatgtccg agacctcctg gccgagcggg ccctgcagga cccggtcgac
     2101 atgcgcctcg tccttgtaga tgaagccggg ggcaccgccg aaggcggcga tctcgggggc
     2161 gatggcctcc tcgctgtcgg ggccggccgt ggtcacctgg atggtgccga tgcggctcgc
     2221 ctcgaggagc tgggcgaggc cgccgagctg gctcgccggg aagtgctggg cgtcgatgcg
     2281 cccgccgctc tcgacgttga gcgtgtcctt ccaggcctcg aaagcattgg tcagaggcga
     2341 gccgatcgcc tcggaatggg cgatgcggca ggtgaactcg gcggcgtggg ccgggcggat
     2401 gaccgccggc atggcgatcg cagccgagcc gagggccgcc ccggtggcga cgaagcgccg
     2461 gcggccgagc gccgttcggg tgttggatcc cgctgtcatc tttctgtgct ccctgatctt
     2521 tcgtttgggt ggccggacgg tatgccgcaa tcgctgatcg ggcaagcgag cgcccacgcc
     2581 tttttttcga tggtccgggg gcggctcgcg gtgcaggatg ggcaggagcg cttaaccgag
     2641 gcttggccgg gcaggcggcg gcccaagcat gaggtgtcga cgatgaggct cgaatcgcgg
     2701 tcgatcggct gcaggttggt gatggctgcc gtcgccctgg ggctgctctt tggggccgat
     2761 gcggccctgg cccagtcacc gcccctgaag ataccctttg tcgacgagtg ggcggcatcg
     2821 ccgcatgccc ggctgggtgc cgaggcgttc aaccactgga acgccgaggg ggaggttccg
     2881 gtgccctgcg cgcgctgcca ttcgacgccg ggcttcctcg actatatcgg cgccgacggc
     2941 acgccgccgg gcctcgacgg tcccgccccg gtcggcaccg tgatcagttg cgtcgcctgc
     3001 cacaacgacg tgacgatccg catggacagc gtcaccttcc cctcgggtgc ggtggtcgac
     3061 aaggtcggcg acgatgcccg ctgcatgacc tgccatcagg gccgggaatc cacggtcagc
     3121 gtcgacgcgc agctcgccgg cgccgagccc gacaccgtct ccgacgagct tcgcttcatc
     3181 aacgtccact accgcgccgc cggtgcgacc cgctacggca ccgaggccag gggcgcctac
     3241 gagtatcccg gcctgaccta tgtcggctac ttcctgcacg acccggactc ccagcgctgc
     3301 gccgattgcc accagcagca tacggtcagg gtgatggtcc aggcgtgcgc gacatgtcac
     3361 caggcgccac agctcaccag gctcgccgat ctcgtgaaaa tccgcaagaa ttcggcgctc
     3421 gacttcgacg gcgacggcaa tgtcgaggaa gggatcgccg aggaaataac gacgctccac
     3481 gcagcgctct atcaggcgat ccagggctac gccctggcga tcggcggcca ggcgatcgtc
     3541 tacagtgccg gcacctaccc ctacttcttc aacgacagca atgccaacgg caaggtcgac
     3601 cccggcgagg ccgtgttccc caatgccttc aagtcctgga cgccgcggct tctgagggcg
     3661 gcctacaact accagtacgt ggtcgaggat ccgggcgcct atacccacaa cccgctctat
     3721 gtcatccagg cgatgcacga cagcctgcaa gacctcggca ccagggtgga ggtcgacatg
     3781 gcagggatgg cccggcccga ggctgaatag gagccgggct cgtctcgcgc cgcgtcgccg
     3841 gcttgccggg acagggcgac ccggctatcg atcggccatg gccgcgttcg agctcgcctc
     3901 cgacagcctc gccctgccgc cgggggcctg ggccaggacc catcggcgcc ggctgcggca
     3961 tgacgggcgc accgtcctcg ccctgacgca gggcgcgttt cggccctatg tctttccgct
     4021 gatgacgccg gccggcttcg ccgtcaccgc cgaggcgccg gccgaccatc cgcaccatgc
     4081 ctcgctctgg ctcggcgccg accacgtgca tgccctgacg ccaacggccc cgggggcggt
     4141 cgaggagtac acctacaatt tctacaccga cgagaccttc cagggccggg cgcccgggcg
     4201 gctgatcgag cgaggctgcg agggtgccgc ggacggcgat gccttcgtca tcgaccaggc
     4261 gatcgactgg caggggccag aggaatgggc agcggcagcg gggcggctgg tcgcccggga
     4321 gcggcgggtg acgcgggtcg ctctcggccc cgggcactac cggatcgacg tcacctcgac
     4381 gctcaccgcc ggcgagcggg cgatccgtct ggggcccacg cgccatgcct tcttcaacgt
     4441 gcgggtcgcc gacgggctga tggtcgcgaa tggcggccgg gtgcaggacg atcggggccg
     4501 tgcgggcggc gctgcgatca gcggcgaggg tgcccgctgg gtcgatttct ccggtcccgt
     4561 cggcggcgga gagtgggccg gggtggcggt gatcgccgag ccgatcggcg accgggcacc
     4621 ttcatggttc gtcgccgatt ggggtgtcat cacagtcggc ccgttccgct ccaaggcgct
     4681 cctgctggag ccggggcagg acttcacggc gcattttgcc gtcatcgtcc acgacggtga
     4741 cgcggaggcc gccggcatcg gccggctcgc cggggactga cgatcagtga ggaaggaacg
     4801 atcatgcata ttgcccgcta tctctacgaa ggcgaggtcc gctgggccgt cgtcgagccc
     4861 gacgggctgc accgcctggc cggcgcgcct tatgccggca tcgagcggac gggtgccgtc
     4921 gatgcgctgc acgcggcccg gctgctctgc ccgatcgagg cgccccggat cttcgccatc
     4981 ggcctgaact atgtcgatca catcaaggaa tcgggcagca ggacgcccga gatcccgctc
     5041 gtcttcatga agccctcgac cgccgccatc ggcccgggcg agccgatcgt ctacccgctc
     5101 gagggccagg aggtgcattt cgaggccgag ctcgccgtcg tcatcggccg gcgcgggcgg
     5161 cgcatcgagc gggaccgggc gctggatcac gtgctcggct acacctgcgc caacgatgtc
     5221 agcgagcggg tgatccagaa ggccgagatg gcgcagggtg cgctcgtgct ctgcaagggc
     5281 ttcgacagct tctgtcccct cggcccgacc atcgccacgg ggctcgaccc gcgcgatctg
     5341 aagatcggtg cccgggtcaa tggcgaggtg cggcaggcga gctcgacctc ggacctgctc
     5401 ttctcggtcg ccgatctcgt cgcctatttc agcaccgcgc tgaccctcct gccgggcgac
     5461 gtgatcctga ccggcacgcc ctcgggcgtc ggcccggtga agccgggcga tgtggtggag
     5521 atcgaagtgg aaggcgtcgg cgtgctcaag aaccccgtgg tggcggaggc ctgacagcct
     5581 cgcgtccccg gctgtcagcg ggccacgagc agcagcagga tgccgccgac gatcacgatg
     5641 cagccgagga gctcgctctt gttgatcgcc tccttgaaga cgagccacga ggcgaggaag
     5701 gtgaagacga gctcgatctg gcccagcgcc cgcacatagg cgaccttctg cagcgtcatg
     5761 gcggtgaacc agccggccga gccggcgacg ccgacgaggc cgacccagat cgcgacccgc
     5821 cagttgacga ggctggcgcg cagctggtcc ggctcgcgcc gccacatcca caagaccatg
     5881 acgaaggtct ggaagagcgt cacgaaggcg agcgtgaagg ccgcctgcat gatgaagctc
     5941 ggcccggcga gtgctgtcga cgcctgccgg tagagcacgg ccgacacgcc gaacgacgcc
     6001 gccgaggcga gcccgatcag ggccgggcgg ccgctgagcg atgagatgac cgaccgcagc
     6061 gtcagcggct ggcgggcgat cgagatcagg acgacgccgg cgacgccgat gaggatcgcg
     6121 gcgacggctc cggccgtgac ggtctcgccg agcaggacga ggccgaagat cgccgcctgc
     6181 accggttcgg tcttcgaata ggccgtgccg acggcgaagt tgcggtagga gaagaggtgc
     6241 accagcagga aggtggcgat gatctgggcg aggccgccca gcgcaccggc ggcgaagaag
     6301 gtgacgttcg gttccggcag cttgtggccg agcccggcat gcaggacggc gagatagagg
     6361 agggcgaacg ggaagccgta gccgaagcgc acgaaggtgg cgccggtggt gccgagccgg
     6421 cccttgagat gcttctgcaa ggccgagcgc aggttctggc agaacgccgc gaagatggtg
     6481 atcggaatcc agagctcgag caggacgcgg ccgcctttca cgcgagcgcc gaggtggtgg
     6541 ccgggcgctg agcctttcgg cttctacaag gcgcgcagcc aggcttccca gcggaaatcg
     6601 tgcatgatcg agcgggcgcg gagatatttc cacgtcccgt atttgtgata gtcgaagtcg
     6661 agggccgaga tctgctcctg gaccatggcc caggtcgccc atttgacgtc ggccagcgcc
     6721 cgcatcacgg cgacgcgggc gaccagctcc ggccggagct cgccggcata ggcctcgagc
     6781 agccgggcgg ccgtgtcctc ggtgaagaac atctcgccga gccagacgcc gagctcgtag
     6841 gagcgctcgt tcatcgaggc gaactcgtag tcgacgagct tcatgggggc gtccggcttg
     6901 accaggaagt tgccgggcat ggggtcgttg aagcagggca cgaggtcgag gccggaagcc
     6961 aggaaggcgg accgggcatt gccgtagagc cggctcagcc agggccagtc ctcgggcagg
     7021 ctgccgccga gcccggccac ctgggcgatg tgctcgtcga tcatgtcgaa gatggtcttg
     7081 gtcaggccca gcccctcgcc ggcgtggaac tggcggtagc agtcgacggc ggcgagctgg
     7141 atggcctcgt cctggaaatc ggcattggtg caggcgcgat agccttcgag caattcggcc
     7201 acctcgagcc cggtttcggc atcgaaatgg accacctcgg ggccgagccc cttggcgtgc
     7261 gccagccggg ccgcctcgtt ggccgccaca cggtcgacga acatctcggt cccggcaccc
     7321 gggatcttga ggaagtagcg gcgctcgtcg ccggcgagcg tgatccgcca gttgctgttg
     7381 gagatgccgc cggtcatcag ggcgtagctg gcgccctgtt cccgccaggc cggcaccctg
     7441 gcgatggccg cctcgacggc gcgctccgcg tcggtctcgg cctcgccaaa gtttcgctcg
     7501 ctcatctcag accctccgca cccgttcctc gaagcgctgg tcgcgcatcg ccatgcggca
     7561 gcgcaggaag cgccattcgg catatttgag gaactcgaga tcggtcctcg ccgaggttcg
     7621 ggcgaggatg gcgccgatca ggccccagcg caggtcgtcg gcgaccccgt agaggcggga
     7681 gcggttgaag aggcgctcgt cgaacctgcc gtggaacgcc tcgaagctct ccctggcggc
     7741 cgggtcgaag gtgttggcct cgaccaggaa gctgccgagg tcctcgaacg ggtcgcgatt
     7801 ggcggcgagg tcgaaatcga cgagaaggac cccgccggcc tcgtcgatca tgacgttgct
     7861 ggcattgccg tcgccatgcg ccggcacggt gtcgatgccg gcggcccgga tggcctgggc
     7921 cgcggtgcgg atgttgtcga ggagccaggc atggtcggcc ggcagggcga cgcccgctgc
     7981 gccggcccgc cggagcaggc tctcgatgtg gtcgaagacg cccatcgtcc gcggcagagg
     8041 gggcccggcc tggaaggccc gcatggcggc gagcacggca tcgcggatgg cgggctcggc
     8101 gaggcggttc agggtggccg tgcgatgcga tccggcaaga tcccgcatga cgaggtcgcc
     8161 ggtcagggga tcggccgcga cgacctcggg gccgacgccg agcgtgcccg cctcgcgcgc
     8221 ggcggccacg acgctcgccg ggtcggcgaa gagggcggcg tcgggatcgg cgatcttgac
     8281 ccagaggctc tcgccggttg ccgcatcccg ggcgcgcagg ctggtggcgt cgacgccctg
     8341 gttcatgggc gaggccaccg gggccacgcc cgggcggagc gtcggctcgc ggcctgccca
     8401 gccgggcacc gtggcgacga gtgccctggc cgcttcgagt gcgtcccgcg tgctcatgat
     8461 gcggtctccc cggctttggc gaggtcgggt ccctcgacca gcagccgttc gagcatgccg
     8521 gtgagcaggt cgaggccgag ggcgaggtcc tcgggcctgg tgtcctcgag gggcgaatgg
     8581 cagatgccgc cgatgctcgg cgtcgccacg acgatcgagg ggcagagccg tgccatgggc
     8641 acggcgtcgt gggcggcgat ggtgtcgagc cgcatggccg gctcgccgag cccggttgcc
     8701 acctcgttgg cgaggcgctg caggccgggc gcgaagcggc cggggcggcg cagctcgtcg
     8761 cgggcgaagg accagccgac gccggcgcgg gtcgcctccg cttcgatcag cgcctcggtc
     8821 cgggcgaagg cggtctccag cacggccgga tcggcggagc gcagctcggc gaagatggtc
     8881 acggtcgagg gcacgacatt gggcgagttg ggcagggcct cgatccgccc gaccgaggtg
     8941 tgcagctcgc ccgggggtgc catgtcgacg agctggcgga gcccggtgat gacgccggcg
     9001 gcggcgagca gcgcgtcctt gcgctcggcc atgggtgtgg gccccgtgtg cgccggcaca
     9061 ccctcgaagc ggatctccag cttgcggcag ccccaccagc gatcgaaggc ggcgaggcgc
     9121 cggccggcgc gctcgagcag cgggccgcat tcgatgtgca gctcgacata ggcggccggg
     9181 cgcggcgccg gctggtcggt gccgagatgg ccgatggcct cgagggcggc cctgacggtg
     9241 atgccgtccc ggtcttcccg ggccagcgcc cagtcggtgt cgatggcgcc gacataagcc
     9301 tgcgagccga gcacgctcgg ctggaagcgg gcaccctcct cgttggtcca ggcgacgacg
     9361 gcgaggttca tcgccggtgc cgccagccgc tggcgcagct cgcgcgccgc gatcaccgag
     9421 gcgatgacgc cataggtgcc gtccagccgg ccgccattcg gctggctgtc gacatgcgag
     9481 ccggtgagca gccagggggc ctcggggccg gcgatcccgg cgaggccgaa gatgttgccg
     9541 atggcgtcga tgcgcacctc gaagccggac tcgcggagcc aggcgaccag cgcgtcgcgg
     9601 gcctcgccat tggcggcggt cgcctcgggc cggtggatgc cgccggcctt ggtggcgccg
     9661 aaggtggaga accgggcgat ttgctcgtgc agctcccgat ggatgtggtc cggccgcgtc
     9721 attgcccgct cccgcctcgc tcgatctcgt cacgcccgct tgggctcttt gcccgatttt
     9781 gtgggttttg accgaatgat ccctaaaagt cgttgacgca tcgggcaagt ctcaaatagc
     9841 gtcggcgcgc caaaggtcgg tcgacgccag caataaggcg caacaagcag gaagggtcct
     9901 ccccatgtcg agtctcctaa tgcccggcaa gcgacgcttt cggacgaacc gtcggcgggt
     9961 gctcggcggc atggccgcgg cgggcgggct cgcggccttt ccggccccct acatcaagcg
    10021 ggcctggggc caggcgcgca accacatggt cttcgccagc gccgagccgg taacgggcaa
    10081 ctgggatccg accagccaca ccacgctcgc ccagatcaat ctcgagggct tcgtcttcgg
    10141 caagctgttc cagacgccga tgcggccgga gaaccccgac gagctggtct tcgagctggc
    10201 caccagccag gaggtgctcg acctccacac catcgagtac aagctgcgcg acggcgtcac
    10261 cttccacaac ggcgcgccct tcggcgccga ggacgtcaag gcgaccttcg aatatgcgtc
    10321 gcagccgggt cgtccggccg cctggtatcc gggcccggtc gaggtcgagg tggtcgaccg
    10381 cctgaccgcc cgcgtccaca ccgagatcgg cgatttcccg gcatcggcct tctatttcct
    10441 cgccggcttc ctgccgatca tgtcgaagga cgatgtcgcc gatcccaccg tcctgcagga
    10501 gcggccgaac ggcaccggcc ccttcaagtt cgtcgagcag cgcggcaaca ccacggtgct
    10561 ggcggccttc gacggcttca tgcatggccg gccgatcctc gacgaggtgc atttcgccta
    10621 tgtgggcgat gccaccaccc gcgttctggc cctgctctcg ggcgaggtcg acgtggtcga
    10681 gcggctcgag cccgagcagt acgagacgct ggccggcgag agcggcgtcg ccctctcgcg
    10741 caccatctcg accgagaaca agtacctgca tttccgctgc aacaaggcgc cgttcgacga
    10801 cccgcgcctg cgcatggccg cctgccacgc catcgaccgc gagctcgtga ccgagatcct
    10861 cggcgtcgcc ggtcatgcct cctcgaacta catctcgccg gtcaagttcg gctatatcga
    10921 cctgccgaac tatccctctt acgacccggc gcgctgccag gagctgctcg ccgaggccgg
    10981 cttcccgggc ggccagggcc tgccgacgct cgaatacctg accagcgacg gcttctaccc
    11041 gaagaccaag gaatatggcg aggtgatcac ggccctcctg caggagcagg gcttcccggt
    11101 cgagctgacg gtcatggaga ctgccgcctg gctcgagcgg atctaccagc gcgagggcca
    11161 ggagccctac ggccatctcg tcgatgtcgg ctggtcgacc ggctcgcccg agcccgacct
    11221 cgtgctccgg ccgatgttcc attcgagcgc cggcccgcgc ggcggcctga tcaacggcat
    11281 cgccgacccg gcgatcgacg ccgtgctcga ccagcagcag gccgagaccg acaccgcgaa
    11341 gcggcgcgag ctgatccaga ccgtggcgac gccgaagctc gccgagctgg tgcccagctt
    11401 ctcgctcttc acctccgtcc tcctgcacgg gcggcgggcc ggcctcgagg ggctgcactt
    11461 ctatcccaac ggcccgatcg acgcgtccaa ggccgagtac aaggcctgat cccgggacgg
    11521 caggcccgtg ggcttcgcgc tgaccttctt cgcccggcgc ctcgggcagg gcgtgctgat
    11581 cgtgctcctc gtctcgttca cgatcttcgc cctcctgcgc gtcgtgccgg gcgatccggc
    11641 gcggatcatc ctcgggccca tggcgaccga cgaggtggtc gagcagaccg cccgcgacat
    11701 gggcctgcgc gacccgatcc cgctccagta catgcgctat ctggggcagg tggcgaaggg
    11761 cgacttcggc acgtccttca tcaaggccaa gggcggcggg tcgatgggcg gcggccgtga
    11821 cgaggcccgg ggcgagcgcg cacgggtgct cgatctcgtg gccgagaccc tgccgctgac
    11881 gctgcagctc gccggcctcg ccctcgtctt caccgcgctc atcgccctgc cgctcggcat
    11941 cgccggcggg ctgatggcgg ggcgctggcc ggacaagctc gccctctacg cctcgtcgat
    12001 cttcgtgtcc ctgccgaact tctggctcgc catcgtcctg gcgctggtgg tgaccgccaa
    12061 gctcggctgg atcccctcga tcggctacca gaacatcgcc tacgccatcc tgccggcctt
    12121 cgtcatcgcc atcgagatgt cgccgctgtt catccgctcg ctctcggtct cgatcgccga
    12181 caccatgcgc caccccttcg tcgagctggc ccaggtgcgc ggcctctcac ccaggcgcat
    12241 cttcagccat cacgtgctgc gcaacgccgc cgtgccggtg ctcaatctct tcggcgtgca
    12301 gatcggtgcc ttgctcggcg gcgtcctggt cgtcgagtac atcttcgact atccgggctt
    12361 cggcctgctc accgtgcagg cggtgctgca gcgcgacttc ccggtgatcc agggcatcgc
    12421 catcatctcg acctcgatct tcgtcgtcat caacattctc gtcgacttcg tctcggcgca
    12481 gatcgacccc cggatcgagt actgatggcc gcctccatcg acgagccggc agcaatcgcc
    12541 gccaccgccg tcgcggcgac gccgggccgg cggatgtggc tcgccacctg gtcgcagacc
    12601 gagttcaaga tcggcctcgt gctcttcctg ctgctcgtcg gcaccgcctt catctatccg
    12661 cttctgtcgg acatcgaccc gaccaagctc tcggtccgcg agcgcttcct gccgccggtc
    12721 ttcctcgagg gcgggacctg ggcgcatccg ctcggcaccg accagctcgg ccgcgacctc
    12781 ttcgtccgct cgctcgtcgg cctgcagaat gccctgatga tcagcgtcgc ggtggtcgtc
    12841 ctgatgttcg ccttcggcgc cacggtcggc ctcgtcgccg gctatcgcgg cggctggatc
    12901 gacaccatcc tcatgcggct gacggatgcg cagctctcca tcccgctgat catcctcgcc
    12961 atcatcatcc tgaccgtgtc gcggccaacg cccttctcgg tcatcgtggt gctcgccttg
    13021 gccggctggc cggtctatgc gcgggtgacg cgcagcgtga ccctggccga gcggcggcgc
    13081 gagtatgtcc ggggtgcgaa gatcctgggc gcttccgaca gccggatcat gtggctcctg
    13141 atcgccccca acatcctgcc gccgatcgcc ttcgtcgcgg tgctcgacat cgcccggatg
    13201 atgatcttcg aggcgatcct cggcttcatc ggcatcggca tccagccgcc gaccccgacc
    13261 ttcggcacga tcatcgccga cggccgcaaa tacctgatca atgcctggtg gatcgccacc
    13321 atgccgggcg gcttcctctt cctcacgctc ttgagcgtca acctcatggg tgccgccctc
    13381 gagcgcgcgc gcaaccgcct gctgcgcggg accatctgag cgatgtcgga acgcctgctc
    13441 accgtcgaag ggctcagcgt cgacctcgtg cgcgacggcg gcttggtgcc ggtgctgcgc
    13501 gacgtctccc tggcgctgga ggggcggcag gtgctcggca tcgtcggcga gagcggctcc
    13561 ggcaagaccg tgctggccaa ggcgctggtg gggtgggtcg ccgagccgct ggtggccagc
    13621 cgcggcgtcg tccggttccg cggccaggac ctgctcgcca tgccgcccga ggcccggcgc
    13681 atcctgcgcg gcagcgctgt cggctatatc ggtgccgatc cgggcagcag cttcgatccg
    13741 accctgccgg tcggccggca gatcgtcgag aaggtgcagg cggtgcgcgc cgacatcgac
    13801 gcccgggcgg cgaagaagcg tgtgctcgac ctcctggatc gcgtgcacat cccctcggcc
    13861 gcccggcggt tcgacgagtt cccgcaccag tattcgggcg ggatgctgca gcgggcgatg
    13921 atcgtcgacg cgctggcctc cgatccggcg ttcctcgtct gcgacaacat cacccagcca
    13981 ctcgacgtga cggtggcggc gcagatcatg cgcctcctgc acgagctgcg ctccgacctc
    14041 gacgccggga tcgtcttcat ctcgacctcg ctgccggtgg tctgcgacat cgccgacgag
    14101 ctcgtggtgc tgagccgtgg cgagatcgtc gagcgaagca cgccagcacg gctgctggag
    14161 gcgccggcgc acccctacag ccaggacctg atcgccagga acccgatcat ctggggcacc
    14221 ccgcccttgc ccgtcccggc cgaggcgtcg ccccgggtca tcctcgcggt ggacgacgtc
    14281 accaagacct atgtcgtgcc ggaccgcacg cggttcttcg ggacccaggc ggtcaaggcg
    14341 gtgcgcggcg tcagcttcgc cgtgcacggc ggcgaaaatc tcggcctcgt cggcgaatcg
    14401 ggttgcggca agtccaccct ctcccgcctc ctgagctggg tcgagcagcc ggaccagggg
    14461 cgcatccgct tcaagggcca ggacatcgcc ggcatgtcgg cgcagcagct cctcgccatg
    14521 cgcaagggct tccagctcct gctccaggac ccgttcaact gcctgccgcc gaacctgccg
    14581 atcggccgct cggtcgccat gtcgctgcag atccacggcg ggctcggccg caaggcgatc
    14641 gccgagcagg tgcgggcggt gatggccgag gtcgggctca aggaggacga ccgcgagcgc
    14701 ctgcctatcg gcatgagcgc cggccagcgg cagcgggtga acatcgcccg ggccctcatc
    14761 ctcgagcccg agctcttgat cctcgacgag acgctctccg ccctcgatcc gatggagcag
    14821 gcgcggctcc tcgacctctt cgagcggctg caggcgcggc acggcttcac ctatctcttc
    14881 atcagccacg atctcgccat ggtgcgccgg gtctgcaccc ggatcgccgt catgtatctg
    14941 ggcaaggtgg tcgagctcgc cgacacgcgc agcgtcttct tcgagccgac gcatccctac
    15001 acgcgcgccc tcctttcggc cgtgccgacg ctcgaggaga agccgttcaa ggcggcggac
    15061 tgcctgctcg agggcgagcc gccgagcccc atcgacctgc cgcaagggtg cagcttcgcc
    15121 gcccgctgcc cgcatgcgat gccccgctgc cgtgtcgagg agccgccgct cgtcgatctg
    15181 ggcggcgggc gtttctcggc ctgctggctg aacgagccag aggtcagccg ggcggcctga
    15241 agggaagcgt tcatggccag cgacgaatcg ggaatgtggg atttcatcgt cgtcggcgcc
    15301 ggcggcgccg gggccgtgct ggcggcccgg ctgagcgagg atccggcgac aagggtattg
    15361 ctgctcgagg ccgggcccga ctaccggtcg gccgagcagc cgcccgagat ggcgagcccc
    15421 aaccccttca acatcatcct gcccgaggcc ttccaggcca cctacatgtg gccggcgctg
    15481 ctggcgcggc gcaccaagcg gcaggcgcat cgcctctact ggcgcggccg cggtgtgggt
    15541 ggcagcaccg ccatcaacgg ccagatcgcg atccgcggcg tgctcgatgc cttcgacaac
    15601 tgggccgagc acggctgcga gggctggtcg gcggccgacg tgctgccggg cttcaaccgg
    15661 ctcgagaccg acgaggactt cgccggcgaa gcctatcacg gcggcagcgg gccgatcccc
    15721 gtccatcgca cgccgccgga tgcctggggc cccgtcgacc gggcgctgcg cgatgcggcg
    15781 ctggacctcg gctatccctg gacccccgac ctcaacgcgc cgaccgccga gggtgtcacc
    15841 acctacgcca tcaacagcca gggcggccgc cgggtctcga ccaatgacgg ctatctcgag
    15901 ccggcgcgcg gccggccgaa cctcgaaatc aagggcggca tgctggcgga ccgggtgctg
    15961 atggacgggc ggcgggccac cggcatcgcc gccatcggac cggaggggct ggcaacgttc
    16021 gacctcgccc ccggcggcga agtgctgctc gcggccggcg ccatccacag cccgcccatc
    16081 ctgttgcgta ccggcattgg cccggccgcc cacctcgccg agcacggcat cgccgtcatc
    16141 cgcgacctgc cggtcggcga cgggctcttc gaccacccct tcgtccggct cgagctgaag
    16201 ctgagggagg agctgaagcc gacgagcgtc gatgcccggc acaccaattg ctgcgtcaag
    16261 tacagctcca acctgccggg cggctcgctc gccgacatga tcttcttcgc catgaaccac
    16321 ggcggcgtcg gggtcgccca ggacatggcg cagttcggcg aggccggcat ccattgcgcg
    16381 ctcttcgagg cgcattcgcg gggacaggtc aggctcgcct ccaccgaccc gcgcacgcag
    16441 ccggtggtcg acctgaacat gctggacgac gagcgggacc tcgcccggct gcgcgatggc
    16501 gcaaggcggc tgatggagat cggcgcccat gaggcggtga ccggcaaatg ccgcgaggtc
    16561 agcctcggca ataccggcag gcccatcgcc gatctggtgg aagcgccgga cgaggccgtc
    16621 gacgactgga tcctcgccga ctgctcggac gcgcagcacg gtgccgggag ctgccggatg
    16681 ggggcgttcg actacgagga cggccgttcc gtgctcgacc ccaactgccg ggtgcgcggc
    16741 atcgaggcgt tgcgggtgat cgacgcctcg atcatgccgg ccgactgcaa ggcgaacacg
    16801 gccttcacca ccatgatgat cggcgagcag atggccgagc ggttgcggcg ccagcgggcc
    16861 gggggcggct gatcaggtcg atgtccgagc tggcgcgcca cgcgaccatc ctggcggcgc
    16921 tcggacgcga tggtcgggtc ggggtcgccg cactggcgca agcgctcggc gtcagcgacg
    16981 agaccatccg ccgcgacctg gccaccctcg acgcggccgg gcggctgcgg cgggtgcatg
    17041 gcggtgccgt gcagttccgg ccggacgagg agcagcccat cctcgtgcgc tcgcgcatcc
    17101 ggccgcgcga gaaggcgcag cttgcggcag tcgcccgcgg cctggtcaag gacggcatgt
    17161 cgatcttcat cgacacgggc tccacggccc tggccttcgc ccgcacgctg gccgatgcgc
    17221 gcgatctcgc cgtgatcacc aattcgctcg acatcgcccg tctcctggcc ccggcacccg
    17281 gcctgcgggt caaggtcgcg cccggctggg tcagggccaa tgacgatgcg ctggtcggcg
    17341 gcgacacggt ggcgttcgtg cgccgcttcg tcttcgacat cgccttcatg ggcatcgccg
    17401 cctgcgacgc cgactatggc tggatggact atgccgacga ggagtcggag ctgcgccgcg
    17461 cggtgatcga gcagacccgc tgcccggtcc tgctggtcga cgacagcaag ttcggccgcc
    17521 atgcctcgat ccgcaccttc gatctcacga caccgctcac cgtggtgacc aaccggccgg
    17581 tcccggcacc cttcgccgag atcttcgcaa gcgccggggt cgaggtgcgc caccggctgc
    17641 ggggcggcta gtcggtcagc gcctccgcca gggcgtcgac cagctcgtcc gcgtgttcgg
    17701 gcttgaaggt gaggggcggg cggatcttga gcacctcgcc atgccgcccg caggcgccga
    17761 tcagcacacc acgctgccgg agcccgttga tcacccgccg ggcggccccg ggatcggggt
    17821 cgtcgtcgcg acagagggcg acgccgagaa agagcccggc accacggaca gtgccgatcg
    17881 atgggtggct tgcctgcagg gcctccagct tccgacgcag gtagcggcct gtttcggcgg
    17941 cgttctgctg cagcccttgc tcgcgaagaa cgtcgagcac ggcgaggccg gccgcagccg
    18001 ccacggggtt gccgccgaag gtgttgaaat agccgaactc ggcgaaatag ggggccatca
    18061 cctcgggtcg ggcggccagg ccggccatgg ggaagccgtt gcccatgggc ttgcccatgg
    18121 tgacgatgtc gggggcgatg ccgtggcgct cgaagcccca aaaggcgtcg ccggtgcggg
    18181 cgaagcccgg ctgcacctcg tcggcgatgt agagggcgcc ggccgaccgg acgacggctg
    18241 cggcggcctg gaggaagccc ggcgggtcgg catggacgcc gtcgctggag aagatcgagt
    18301 cggcgatgaa ggcggcgcag ccgaagccgg cctcgtccag ctcgccgatc gcctgccaga
    18361 cgctggcggc gaagccgccc gcgaggtcgg tgccgtaatg ccgcgcatcg ggggcgggca
    18421 cgatccggac atgcgccggc ggccgtccct gcttgaaggc cgagggcgag atctcgctca
    18481 cggccgaggt gttgccgtga taggcggccg ccgtgacgat gatgccctgt cgcccggtgg
    18541 ccgtccgggc gagccggagc gccaggtcgt tggcctcgct gccgctgcag gtgagggcaa
    18601 ggttcgagag cgctgccggc agggtcgcct tgagccgctc gagataggct tccgtcggct
    18661 cgttcaggta gcgcgtgtgg acattgagcg tcgcggcctg tgcggcgatc gccgccacca
    18721 ccttcgggtg gcagtgaccg accgaggcca cgttgttgta gacatcgaga tagcgccggc
    18781 cgtcgcgcgc ctcgagccag acgtcctcgg cccgcacgat gtgcaaaggc tcctcgtaga
    18841 agagcacgga gaccgggccg agattcgcct gccgccgggc cagcagggcc gccgtgcccg
    18901 gatcggcggc agcaccgcgc gccgggtcga aggcgttgag ggagagaagg tcggggtcgg
    18961 tcggcatggg ccggcactct gcgtcgggac cggggcgaag acaaccgtgc ggcgcttgcc
    19021 gcgagccacg ctcggcgggg ccgggggcgc gaacgaggcg gattagtggt ggcaccccaa
    19081 gccggttgcc ggtatcgaac cggcaaccac aatgttctgg tcagggagaa acgtcgatgt
    19141 tcaggcacat tcgcaacggg atcgtcggct cgacggcgct ggcgctcgtc agcgccgccg
    19201 cactggcggc gacgccgccc gacatgctgg tgatcggcac cgacgtcggc gccattccga
    19261 cactcgaccc ggcagcgctc aacgcccgga ccgtctcgga gctggtctcc aacctctacg
    19321 acaatctcct caagctcgac cccgacgacc tgcagtcgat ccagccgatg ctggccctga
    19381 gctgggaggt ggcgccggac agctcgagca tcacgctgaa gctgcgcgac gacgcgacct
    19441 tcgcgagcgg caacccggtc actgccgagg acgcggcctg gaccatccag cgggtgatca
    19501 agctcggcca ggtgggtgcc acggacctcg cccagtgggg cttcaccgcc gacaatgtcg
    19561 agcagctcgt ccgcgccgag gacgagcgca cgctggtgat cgagctgccc gagcaggtca
    19621 gcaccgacct cgtgctctac tcgctcgccg gctcgtcgct cggcatcatc gaccgcaagg
    19681 cggcgctcga gcacgagacg gacggcgatc tcgcccgcga ctggctgcgc gccaatgccg
    19741 ccggcagcgg cccgttcacg ctcgtcgaat ggcggccgga cgacatcgtg gtcaccgagc
    19801 ggcgcgagga ctactggggc ggcacgccgg cgatgcgccg ggtgatcatg cgccacgtgc
    19861 cggagtctgg aaatctccgc ctgcagctcg aggccggtga cgtcgatatc ggccagtatg
    19921 tcggctcggg cgacctcgag gcgctggccg ggaacgagga catggagatc cagcacacgc
    19981 cgggcttcgg cttctactac atcgccctca accagaagga cccggacctc tcgaagccca
    20041 aggtgcgcga ggcgttccag cacgtgctcg actgggaggc cttgagccag tcgtcgatgc
    20101 gcttcaacgg ctttccctgg cagtcgatca tcccgcgcgg catggcgggg gcgcccgagg
    20161 aattcgagga gcacttcgcc tacgatcccg agcgggcgcg cgagctgctg gccgaggccg
    20221 gctacccgga cgggctcaag aagaagctct acccggccgg cgacggccat ctgctgaatg
    20281 ccgaatcgct gcaggccacg gcgcgcctcg ccggcatcga gctggagctg atcccgggca
    20341 accacgtgcc ggagttccgc gcccgcgact tcgaggtcta cacgggcaac tcgggcggcc
    20401 gcctgcccga cccgttcgcc acggcgaccc actacgcctt caacccggac aacagcgacg
    20461 aggcgcagct cagcggctac tacatgtggc ggacggcctg ggacgtgccc gagctgaccg
    20521 agctaaccaa cgaatcgaag cgcgagtcgg accccgagaa gcgcgcccag atcttccgcg
    20581 agatggacga gatgtaccag cagctcgatc cgtcgctgat cgtgttcttc cagcgcgtcg
    20641 acccctacgt cgtccgggcc gaggtcgagg gctatgtcgg ccacccgacc tggtcgaccc
    20701 gctgggccga cgtgaccaag aactagggac cgtcggaaag ggtgggcgtg cccagcgtct
    20761 cgatccttcc cgggccgcgg gcacgccggg cgcggctcgg ccgccttgcc ggccggacgg
    20821 tgtcgctggc gctcaccctg ctcggcctca tggcgctcac cttcctgatg gggcgcctgc
    20881 tgccggccga cccggtgctc gccgtgaccg ggttcgaggt cgaccgtgcc acttatgacc
    20941 gcgtcttcct cgagctcggc ctcgaccggc cggtctggca gcagttcctc gcctatgtgg
    21001 gcggcatctt ccagggtgac ctcgggcgct cgaccacgac gggccagccc gtcgcggtcg
    21061 acctgctgcg ggtgttcccg gcgaccttcg agctggcgct gaccgcggcc ctcgtcggct
    21121 cgctgctcgg catcccgctc ggcatcgtgg cggcggtcaa ccgcgaccgg atgatcgacc
    21181 acatcgcccg gatcatcggc ctcgccggct actccatccc gatcttctgg atggggctga
    21241 tggcgctcct ggtgttctac gccaagctgc gctgggtagg ggcctcgggc cgcgtcgacg
    21301 tctattacga ggggctggtg ccgtcccgaa cggggctcct cttggtcgat tccgccatcg
    21361 ccggcgactg ggcggtgttc aggagcgcgt tcgcgcacct gatcctgccg gcctccatcc
    21421 tcggctacta ctcgaccgcc tatatcagcc gcatggcccg cagcttcatg ctcgagcagc
    21481 tcggccagga atacatcgtc gccgcccggg ccaagggcct gccgcggcat gcggtcgtct
    21541 ggcggcatgc cttcaagaac atccgcgtcc agctcctgac ggtggtggcc ctcgtcttcg
    21601 ccggcctgct cgacggcacg gtgctggtcg agaccgtctt cggctggccg ggcgtcggcc
    21661 agtacctgac ccgcggcctg cagtacaacg acatgaacgt ggtgatgggg accgtgctgg
    21721 tgatcggcct cctcttcctc gccatgaaca ccatcacgga ctggctctac cgccggctcg
    21781 acccgaggac gcgatgagcg ggacggcccg gcccggcagc gattccctcg gccggcccca
    21841 ggggctcagg gcctggctcg acgccgagga acccaggagc ctgcgccagg cgcgggcgca
    21901 gcgggcctgg cgcggctggc aggccttcct cggccaccgc ctcggcgtgg tggggctcgt
    21961 gctcgtcgtg ggcctcgtgc tggtcgccct cctcgccccc tggctcgcca cctacgaccc
    22021 gctcatccag gtcatggacg agcggctgca gccgccctcg gcagcgcact ggctgggcac
    22081 cgaccatctc ggccgcgaca tctactcgag gatcgtctgg ggggcgcgaa cgacgctgcg
    22141 gatcgccgtg ctggtcgccg tgatcgtcgc ccccatcggc ctcgcgatcg gcgccaccgc
    22201 aggctatctc ggcggcatcg ccgacgagat cctgatgcgg gtcacggaca tcttcctcgc
    22261 ctttccgggc ctnnnnnnnn nnggcggcga tgtagtcgct gccccggcat caacaacgcc
    22321 atcatcgcca tcgccctcac cgcctggccg ccgatcgccc gcctcgcccg cgccgaggcg
    22381 ctgaccatcc ggggcagcga ctacatcgcc gccgtccggc tccagggggc ttcccgcacg
    22441 aggctcatta cccgccacgt cgtgccgatg tgcctgccgt ccgtggtggt ccgcgtcacg
    22501 ctcaacatgg cgggcgtcat cctgacggcc gcaggtctcg gctttctcgg cctcggcgcc
    22561 cagccgccct ggcccgaatg gggggcgatg gtcgccgtgg gccggcagtt catgttcgac
    22621 agcccctggg tggtggcggc ccccggcatc gccatcgccg tgatcagcct cgccttcaac
    22681 ctcttcggcg acgcgctccg cgacatgctc gacccgcgca gcatgtaggc ttttggcgcc
    22741 gggtgcgctt ggctttcgag cgccgggcag gcaaaactcg cgtattcgac ggggggtacg
    22801 gcttggccag gcgaccgacc atgcggcgca acgcggagat cagccgggca cggctcgtgg
    22861 aggcggcgac cgaggaattc gcggctcgcg gctatgatgg agccagggtc gatggaatcg
    22921 tcgcccgggc cgaggtctcg aagaacctcg tctaccacta cttcgccggc aaggaggcgc
    22981 tcttcatcgc ggtgatggag cgggtctacg tgctgatgcg cgcccatcac caggacgatc
    23041 tcctgaagca gctcgagccc agggcggcga tcgagcggct gatccgctcg gtgcaccggc
    23101 tcttcgtcga gcggcccgag atcatcacgc tcctcaacag cgagaacctg cacaaggcgg
    23161 cgcatatcgc gaagtcaggc acgataagga cgctctacaa cccgctgctg gaagcgctga
    23221 tcgacatcct cgaccggggc tcggccgccg gcgtctttcg ccgcggggtg gaccctgtgg
    23281 acctctacat cacgatcagc gggctctcct atttctacat ctccaacgcc cacaccctga
    23341 ccttcatctt caacgaggac ctgctggcgg aggagcggct ggcgcggcgc gagcaacaca
    23401 tcgtcgaggt ggtgctgggc tatctcgagg cgggccgggc gaaggacgcg gtcaaccggc
    23461 gtccggcgcg cgcggcgttg cccggctgac cagcagcagg ccggccatga cgaggacgaa
    23521 gccgagcgcg tgataccagg cgagggcctc gccgaggaac gccacggcca tcgccgccga
    23581 ggcgatcggc gtgaggtaga gaaaggccgc ggcgcgctgc gggccgagcc gcgacacgcc
    23641 gaagatgtag aacgcgttgg ccagcagggt cggggcgatg gcggcgtagg cgaggatcag
    23701 gagcgcggtg gccgcgtcgg cgcccgtggt cacgtagggc tcgccgccga aggcgagcca
    23761 gaccagatag gtgggcagca gcacggtcag ggtgccggcc gtcgccgtcg cggtggtcag
    23821 cgacaggctg gcaatgcggt cccggtaggg ccgcaggcgg gtggtgaacc aggcgaacag
    23881 gatggcggag gcgagcacgt agagattgcc gatgccggcg ccgaggccga ggaccgcggc
    23941 aggatcgccg cgcgtgacga cgaggagtgc tccggcgaag gcgacggcga gcccggccag
    24001 cgtggtcgcg gcaatgcgct cgcggccctt gagccagaca atgacggcca cccaggcggg
    24061 cgtggtcgag ctgatgatgc cgacctcgat ggcgaggctg taataggcgc cggcgatcag
    24121 caggacggag aagagtccga cgccgatcgt gccgagcagg aggaggtcgc gccagcgctc
    24181 gcgcagcacg ggccagtcgc ggcagagccc cggccaggcg acggcgatca ggatcggcag
    24241 ggcagcggcc cagcgccaga agacgaaggc ggcgagcggc acgatctcgg tcgagccccg
    24301 ggcgaccacg tggttgctgc cccagagcgt ggcggtggcg agcagcgcca gaccgccgag
    24361 cagagggccg ccgagcaggg cgccgccggt caacaggcgc tcagccgccg tgctggctgc
    24421 cgccttcgcc acgctgcgag acgtgccagg ggagcatcag ccgctcggcg aagtcgaggg
    24481 cgtagaagag ggcggtgccc atgaggccga gcagcaccag cgagacgaag acgcgggtgg
    24541 tgtcgaactg gccctgcgcc accagcacga gaaagccgag cccgttgccg ccggcgacga
    24601 actcgccgac gatcgcaccc acgagcgcga aggagatcgc caccttcatc ccggcgaaga
    24661 ggctgggcag cgcattgggg aagcggatct tggccaggat ctggagctgc gaggcccggg
    24721 cggcacgcgc caggttgacc atgtccggat cgaccgagcg gagccccagc accgtgtcga
    24781 tgacgatggg aaagagcgcg agcatcacgg cgaccgcgat cttgggctcc atgccggtgc
    24841 cgagccagat gacgaagagg ggagcgagcg ccaccttggg cacgctgttg agggcgacga
    24901 ggagggtgta gatcgtccgc tccaagaggc gcgaatagac gatcgcgacc gagagcgtga
    24961 tgccgagcgc caccgagatc gtgaaggcga gcagcgtcgt ccagagcgtg tagcccatgt
    25021 gccggatgaa gagctcgggc gtggtcagga actcggcgag gatggcgctc ggcggcggca
    25081 ggaagatcct gggcggctcg tagtgccaga cgccgagctc ccagatggcg acgagtgctg
    25141 ccagcacgag gagcacgtcg cgggtgcggc tttcgcccat caggaacttg cgccagccgg
    25201 tcattccttg aggatcccga gctcggcgaa gtggtggcgg atctggtgca cataggcgcc
    25261 gaaggccggc gtctcgcgga tggaaagcgg gcgcgggcgc ggcaggtcga tgtcgatcac
    25321 ctcgacgatc cgcccggggc cgcgcgacat catcaccacc cggtcggcga gatagaccga
    25381 ttcgacgatc gaatgggtga tgaagagcac ggtcttgttg gtgccctgcc agatccgcgt
    25441 cagctcgaca ttgaggtcgt cgcgggtcat ggcatcgagc gcgccgaagg gctcgtccat
    25501 cagcagcagc tcgggatcgc agagcagcgc ccggcagatg gcggcgcgct ggcgcatgcc
    25561 gcccgacagc tcccagggat ggcggtcctc gaagccctcg aggccgaagc gggcgaggag
    25621 ctgcatggcc ctctcccggt agtcggcgcg cggcagacgc cgcagctcgg cctgcagcag
    25681 cacgttgtcg aggatggtcc gccagtcgag cagcacgtcg cgctggaaga cgatgcccat
    25741 ggcgccgggc gggccctcga ggctgcggcc attgatgtcg atggtgccgc ttgtgatcgg
    25801 cacgaggccg gcgaggcact tcatcagcgt gctcttgccg cagccgctcg ggccgacgac
    25861 gctgacgaac gagccgggtg ctatctcgag atcgatgtcg gccagcgcct cgacgtcgcc
    25921 gcgcggtgag gggtagacct tcgccacgcg gtcgagcgcc acatagggct gggtgccctg
    25981 gttcacccga tcctactgca cgaggtcgtt ggtgaagtag tcgtccgggt tccagcccgg
    26041 gccgatcagg ccggccttct ccatgccctg cagcgccttt tcccagtcct cgcgcgcctg
    26101 ccagccgatc ggcttgcccg ccgtcgccgg cgtgtcgaag tagtcgaggg tgatgcggat
    26161 ctgctcgcgc aggatatcgg ggtcgagatt ggcgtccggc cgctgggcga tgatcgcctc
    26221 gacgccgagg tcgagatcgt cccggatggc cgcccaggcg cgctgctcga cggcgaccag
    26281 cctggcgaga tcctcggccc gctcggcgat cgtcgcctcg gtggcgatca acccgtagct
    26341 cgggaaggtg aggcccgcct cgtccagcat catgcacttg gacggccggg atttctcggc
    26401 aaccgggatg gcggagccga tggtcgacat cagcccgtcg gcgcggccgg cggtataggt
    26461 gccccacaag gccgccggat cgacgaacag cacctcgacc gtggtctggt cgagaccgcc
    26521 cgccgccaga tactcgtcga tatagggagc ccaggggctc gccgcgaaga cgacgagcgc
    26581 cttgcccttc agatcggcga tcttctgaat cggcgcgtca cggtcgacca gaatgcagag
    26641 gtccgtcttg cgctggaagc cggcgaagga cttgatctcg gccccctgct cgcgcgcggc
    26701 gccgagcagg ccgagctgga tctggccgac atcgacctgg ccggcattga cgagctgcag
    26761 cgtgttgccc gagccgcggc cgtcctggag gtcgacggcg agccccgcct cctcgaacca
    26821 gccctcgttc atggcgtagt gcatgccggc atgcacgccc cagggcgaga aatcgagcct
    26881 gaccgtcaag ggctcctggg cggcggcgag gcccgttgcc gctgccatga gcgctgtcgc
    26941 cagggtgctc gagaggatcg tccgtcgggc tgccatcgtc gtccctcctt gttgaagact
    27001 acgttctctg cgcgcgcatg ccggcgcgaa gtgccgccgt cgcggccgta tccggcatga
    27061 aattgtcgga caggaccacg ccatgccggt cgcgggcgaa gtcccggctg atcttctcgt
    27121 cgagcgcgtc gatggcgacc agcgccgggt cgcgctcgag cgggtcgccg tagccgccac
    27181 cgccggcctg gtggtggcag aggctgtcgc cggcctcgat gttccgcgtc agcttgccgg
    27241 gcacggcctc ggcattgcgg tccgggttca ggatattgac cgtgagggcg cccggctcgc
    27301 cgccgaagag gccatagggt gcatgcgccg tgcggtcgga gcggagctgc agcatggcct
    27361 cgggggccag caggcggtag gtgcgcgtca ggccgagccc gccccgccac ttgccggcgc
    27421 cgcaggaatc ctggcggtag ccgaattcct cgatgcgcac gggatagcgc gcctcgagcg
    27481 tctcgaccgg caggttcgac atgttctgcg cggggttggt cacaccctcg atgccgtcct
    27541 ggtcgacgcg cccgccccag gcgccgttga tcatgtcgac gaggatgaag ggctcgcccg
    27601 tcgccttgtc gtagccgccg aggcagacga ccgtgttgcc gccctcgccg gccgccatca
    27661 ccttggccgg cacgatctgg gcgagggcac ccagcatggt gtcgacgatg cgatagccgg
    27721 tcagcgcccg cgccgcgacc gctgccggca gccgcgggtt cagcatcgag ccgagcggcg
    27781 ccttgacgtc gatgcagcga aagacgccgg cattgttggg cgcgtccgaa tccatgacgc
    27841 agcgcacgct cagatagctc gccgacttga cgaaggagag ggtggagttg atggccccct
    27901 tcacctgcgg cgcgctgccc tcgaaatcga ccccgagatg gccgccctcg acggtgatgg
    27961 tcgccttgat cgggatcggc tcgtcgctga agccgtcgcc gtcgatgtga tcggtgaagc
    28021 tgtaggtgcc gtccggccag gcctcgatct cgcgccgggt catggtctcg gcgtagtcga
    28081 gcaggccctc gatatagctc tcgacggtag cggccccgta gcgttcgaag aggcgcagat
    28141 actgccgctc gcccatccgg caggtggcgt attgcgagag caggtcgccg atcacgaggt
    28201 ctggcacgcg gacattcttc tcgaacaggc gaaagagcgt cttgttgggc tcgccccgct
    28261 cgtagagctt gagcggcggg acgcggaagc cctcctggaa gatctcggtc gaatcggagg
    28321 cattcgagcc cggcacccgc ccgcccatgt cgcagtgatg ggcaacgacg acggtgaagc
    28381 cctgcagcgc tccgtcgaag aagaccggct tgaacatgaa gatgtccggg agatgcatgc
    28441 cgccctcgta ggggtcgttc atgatcacca cgtcgccggg gtggaggtcg tcgccatagg
    28501 cggcctgcac cgcctcgatg gcgtcgggca ccgcccccag atgcagggcc acggtcttcg
    28561 cctgggcgac gatgcggccc tgccggtcgc agagcgtggt cgagtagtcg agcacctccc
    28621 ggacgatgtg cgagcgggcc gtgcgcatca cgttgtaggc catctcgtcg acgatggtgt
    28681 cgaaggcgtt cttgatcacg gcaaaggtga tcgggtcggc gaagggcgag gcgtcgctct
    28741 tggtcatgac cggccctcca gctcgatgcg cagattgccc gcctcgtcgg cccgcaccac
    28801 ggcccccggc gggatgacgg cggtggtgtc caggctctcg acgatcagcg gccccttcgc
    28861 cacttccggc agggccgagc ggtcgatgac gggggtcgaa acatagccgg attcgcgcgc
    28921 gaacatcacc tgacgctccg acgcaacgtt cgtgccaccg gcggcggccg tgcggtagct
    28981 cgtgaagtcg agcttgccgg cgcgatggcc gcggccgacg acgcgcaccg ccacgacctc
    29041 gacccggtcc cgcgcacggt actcgtagac ccgctcgtag gcttcgagga agcgctcgcg
    29101 caaaggtgcg gcgaagcctg cctcgatggc gccctccacg gtgatgccga gctcggtgtc
    29161 ctggttctcg aagcggagat cgatctgccg ctcgatcgcc atccggtcgg cggtgaagcc
    29221 ctcctcggcg agcgccgccc tggcctcggc cgcgagccgg tcgagcgctg cctggagccg
    29281 cggccccagc gcctcgtcga gcggggcggg caggggctcg acgagctggc gctcgatgtc
    29341 gccggccagc atgccggcgg cggtgaagac gccggcgagg ttcggcagga ggacgtggcg
    29401 gatgccgagc atggcggcga ggtcgcaggc atggacgggg ccgctgccgc cgaaggcgac
    29461 gagggtgaag tcgcgcgggt cgacgccgcg ctcgacggtc accgcacgga tggcgcgggc
    29521 catgttgagg ttggcgatct cgcgcacacc gagcgccgcc tcggcggcgg tgacgccgag
    29581 cggggcggcg acgtgggttg tgatcgcctg ctccgagagg ccggcgtcga tggcgagcgt
    29641 gccgccggcg agctccttcg ggttgagata accgagcacc acgttggcgt cggtgacggt
    29701 ggcgctgtcg ccgcccttgc cgtagcaggc cgggccgggc tcggcgccgg ccgagcgggg
    29761 gcccacatgg atgagcccgc ccgcatcgac ccgggcgatg gagcccgccc cggcgccgac
    29821 ctcggcgatg tcgatggtgg gggcccgcat gaggaagccg ccggccttga tgaagcggct
    29881 cggcgtcgag atgccggcgc ggaactcgta ctcgttggtg cgcgagatgc gcccgccctc
    29941 gatcagcgca gccttcgccg tggtgccgcc catgtcgaag acgatgaggt cgccgatctt
    30001 gccggccgtg ccgagccggg cagcacccgt gaccccggcg gccgggccgg aggagatgaa
    30061 gagcacgggc tgctcctgcg ccacggcgga gcgcgcgagc ccgccgctgg agctgacgac
    30121 gagcagcggc gccgcgatgc cgaggcccgc gagcccggcc gcgagccgcg ccagatagcc
    30181 ccgcatgacc ggcaggacat aggcgttgac cacggccgtg ctggtccgct cgtactcctt
    30241 ggcctcgggc tgcagcgccg ccgagagggt gacctcgaag gccgggaagt gctcggccaa
    30301 gagctcgccg acgcgccgct cgtgggcggc gttgcggtag ctgttgatga agcagacggc
    30361 gaccgaggtg atgccctgct gctgcagccg ccggccggcc tccagcacct ccgcctcggc
    30421 gagcggcgtc agcaccgtgc cgtcggcggc gatccgctcg gtcacctcga gccgggcgcg
    30481 gcgcggcacc aggggctcgg gcttggtcca ggcgaggtcg aacatctcgg gcgtgcgcac
    30541 ccggccgatc tcgaggatgt cgcgaaagcc ccgcgttgtg atcagcccgg ttttcgcacc
    30601 ggtgcgctcg agcagcgtgt tcgagccgat ggtggtgccg tgcagcacct cgaccacctg
    30661 gctgccgtcg acgccggccg cggccatgat cgcgcgaatc ccgtcgagca ccgcggcctc
    30721 gggtgctgag cccgtcgagg agaccttggc ggtcagcacc tcgcccgcac ccttgaccag
    30781 cacgaggtcg gtgaaggtgc cgccgatatc ggcggcgatc ctgaccgtcc gcccgcccgg
    30841 ttccgccaga cccatgcgcc accgtcccta actaaccagt tggttagtac gctaggtgcg
    30901 ggaggacacc ttgtcaatcg gcgggatttg ctcaggcggt ggtgatggcc cgggcgggcg
    30961 ggaaggcttc gagcgcccgg tcgatcgcgg tccggtcgag gaggacgaag ccggtcgaga
    31021 gcccgtcggc gatggtggcg cgcgggtgca ggacggagac ccggcgccag ttgccgggcg
    31081 aagggcggcc gaggcgcggg tcgatgatgt ggccgattcc gccggcggca tccatgaccg
    31141 tgccgaacgg ggccgaggtg gcgaccgcac cgtccgtcag gggtacgatt tcctcggccg
    31201 tgccgaggcc gatgcgccag ggctcgccgg ggctgcgctc gccggtggcg cgaatctcgc
    31261 ccagatcgac cagcacatgg gcgagcccgc cggcgcgcag caggtcggcg atgcggtcgg
    31321 tgacgtagcc ctgggcgatg ccgttcaggg tcagcgccat gccgggccgg ccgaaggcga
    31381 tttcatgggt gtcgaccgcg acggcctcca tcccgacggc cgcgcgggcg gcgcgcaggg
    31441 cttcggggtc cggggcacgg ccggcagcgg cggcctccgc atggcgccgc cagagcggct
    31501 ggactgtcgg atcgaaggcg ccgccggtgg cgtcgtggac cgcccgcgcc tggtgcagca
    31561 gatcgaccag ctcgaagggc gggtcgtcga gccggccggt gccgttcagc cgggcgagcg
    31621 cgctcgacgg gtcgaacagg ctgaagatgc gctcgagccg gcggatctcg gcaactgccg
    31681 cctcgatcag gcgcgtcgac cgggccgcgt cgtcgtggcg gacgacgagc gccgccgggg
    31741 cacccagggc catgcccgac cagcgctgcg ctggcacggc ggcgccggca ccggcgggca
    31801 gcgccaggcc ggcggcgctg gccgcgagaa tggtcaggca gcggcggcgg gcgaggtgcg
    31861 tcatggcgtt cggctttcgg ccgccaggcg gctccagccg agccaggcga tggccagcca
    31921 gacgaggctg cgcagggtca tcgcacccac ggttcgcatc tcgaaggcgc cgcccgtggc
    31981 gacatggacg gcgaaggcca cgaagacgaa gagcgtggcg atggcgatga gccaggcgag
    32041 gcggggggcc cagggccggg cgcgccagag gccgatgccg gccgcgatgt agacgaagcc
    32101 ggcgagcgtg ttgaaccaga gaacgaaggg cacggcattg ccgaccgcgg cccgggcctc
    32161 gggcccgccg aagagcgctc tgccgccgga gagcacggtg gcgatgccga agacgacggc
    32221 gacgacggcg agggccttca gcaggcggct gctggtgttc atgtcgggct tcctccgcct
    32281 gccggcgcgt gcagggccgc ggccgcctcg cgccgcttgc ggctcttgat caagggcggg
    32341 caggtatggg cgtcgtggta gtgctcctgg cagtcgaggc agtagatgca ctcgttgggg
    32401 acgatctcgc ccgaggggtg gatcgcctgg accgggcagt tgacctcgca gatccggcat
    32461 tcccggccgc attgcggccg ccgcttgagc cagcggaagg tggcgagccg ggccgggacg
    32521 gcgagggcgg cgccgagcgg gcagacatag cggcagaaga agcgctcgat gaagagcccg
    32581 gcgccgagca gcagcagcgc atagacgacg aacggccagt cgcgcacgaa gcgcaggatg
    32641 atcgccgtct tgaagggctc cacctcggcg gccagggtgg cagcgctcat cgcatggagc
    32701 gagagcgcga gcaggccgag gaagacgatg tacttggtcg gccagagctt ctcgtgcagc
    32761 ccgaagggca gggcgagctg cggcacgcgc agcaggcggg cgagcttgtt ggtcagctcc
    32821 tgcagggccc cgaacgggca gagccagccg cagaagacgc cgcggccgag gaagagcagg
    32881 gccag
//
LOCUS       DAOTFI010000013        32070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1567_length_32070_cov_7.594721, whole genome shotgun sequence.
ACCESSION   DAOTFI010000013 DAOTFI010000000
VERSION     DAOTFI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 32070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32070
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1567_length_32070_cov_7.594721"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="P5558_02210"
     CDS             complement(<1..407)
                     /locus_tag="P5558_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRY23155.1"
                     /translation="MSREPRVTVWNEFRHEQANPKVAEIYPRGIHEALAGPLRAVGLE
                     VATATLDEPEHGLDRRRLDATDVLVWWGHRAHDEVADEIVQRVRQRVLDGMGLVALHS
                     AHFSKIFKTLMGTGCDLKWREAGERERLWVVDPT"
     gene            complement(404..1486)
                     /locus_tag="P5558_02215"
     CDS             complement(404..1486)
                     /locus_tag="P5558_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY23156.1"
                     /translation="MVRLVQIGLGGWGRDWAREVLPAVPEVEPVAFVDGDPATRELAR
                     EALRIAPAKLFASLDEAVAATGPTAALVVVPLAAHAAVTEAALRRGLHVLVEKPFTET
                     LSEAAALVELAAATKLHLMVNQNYRHFPTPRAFRRLVADGALGHVASIAVDFWKAWDE
                     NYRYFFLAEPLLSDMAIHHFDMMRYVLDDEPEAVTAMSWSEPGTPFQGRPAAAALIRF
                     RRGTVVSYRGTWISRGPDTPWGADWRIDGSAAAALGRFRGNQGDRRGADRVTLYTAHD
                     KSRPLELEPLPRIDRAGALAAFAAWVGEGRPAAGLSTGADNLESLCLMHAAIRSAAEH
                     GRLVPLAEIANAAGIMDSTATEEGGK"
     gene            complement(1517..2005)
                     /gene="ruvX"
                     /locus_tag="P5558_02220"
     CDS             complement(1517..2005)
                     /gene="ruvX"
                     /locus_tag="P5558_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRY23157.1"
                     /translation="MSPEASTSAAAAFAAGLPATGSLLALDVSKRRIGLAGTDPERRL
                     VTALSTFERRGLAADTAHLSAVVRERGVVALVLGLPLNMDGSEGPRARSARDTAQNLA
                     RALDLPHFLQDERLTTEAVRQAIAEGRLARPKPGAAIDHFAAAVILEDALRALRAAGP
                     VC"
     gene            complement(2002..2922)
                     /locus_tag="P5558_02225"
     CDS             complement(2002..2922)
                     /locus_tag="P5558_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23158.1"
                     /translation="MTAKRKPAAKRPGAELGTLLDAGVADDRQAMEALYRAAAPELYG
                     RARAAVEGPAADAALVAAFLEIFATAKERDTAARPAAWLAAVLDRHLPTGATPPKAAV
                     APVQPPPEVWQRLDIAIGLKRLDRHVKPGVATMARGRDPMPNEMREEQDRALRRWRLG
                     AVAAGSVALALALVLLGDRLLPPAVDTPAEAAPAPVALEHDRVGLLQPLAPGRVWRVG
                     LAGNQLSVTPVPPLVLTDDRVLRLWAMPDPDGSPQALGSLDAGRTTTLELPEEYQADG
                     LGLGVSVESAEAPPMALPTAPFLFYGRLLP"
     gene            3084..3371
                     /gene="gatC"
                     /locus_tag="P5558_02230"
     CDS             3084..3371
                     /gene="gatC"
                     /locus_tag="P5558_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRY23159.1"
                     /translation="MSLDKATVRKISELARIEVPESDLEPLAQELSAIIGWIEQLAEV
                     DTDNVAPMRSVMPITHAWRADEVTDGGMQEAITQNAPSAHDGYFVVPKVVE"
     gene            3371..4843
                     /gene="gatA"
                     /locus_tag="P5558_02235"
     CDS             3371..4843
                     /gene="gatA"
                     /locus_tag="P5558_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRY23160.1"
                     /translation="MADMTDLGLLEARHRLAARELSAKELTEAHLAAMIEARPLNAFI
                     TETPELALAAAEASDARLARGEGGLLEGLPIAIKDLFCTKGVQTTAGSRILEGFVPPY
                     ESTVTQRLWAEGAVPLGKTNLDEFAMGSSNVTSHFGPVRSPWTRASDNADLVPGGSSG
                     GSAAAVAARIALAATGTDTGGSIRQPAAFCGITGIKPTYGRCSRFGVVAFASSLDQPG
                     AFGRSVEDSALLLQAMAGYDPNDSTSADLAVPDWTASLKGKVKGLRIGVPKEYRVDAM
                     PAEIEKLWQQGADWLRAEGAEIVEIALPHTKYALPTYYIIAPAEASSNLARYDGMRYG
                     LRVEGETLADMYKKTRAAGFGTEVKRRVMIGTYVLSAGYYDAYYLKAQKVRRRIVEDF
                     EQAFGKVDAILTPTAPSAAFRAGEKMDDPVAMYLNDVFTVPSSLAGLPAISVPAGLSA
                     EGLPLGLQVIGKAWDEQTVLDVGLAIERAAAFAVMPYGLG"
     gene            5052..6416
                     /gene="chrA"
                     /locus_tag="P5558_02240"
     CDS             5052..6416
                     /gene="chrA"
                     /locus_tag="P5558_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRY23161.1"
                     /translation="MPLNGPVHAIPFPEALRVWLRVALLSFGGPAGQIAVMHRILVEE
                     KRWVGEARFLHALNYCMLLPGPEAQQLAVYLGWLLHRTKGGLVAGTLFVLPGFLSILA
                     LSWVYVLLGDLTLVEGLFFGLKAAVLAIVLDALVRIGRRALKNNVLVGTAVAAFVAIF
                     VIGLPFPLIVLGAALAGFVATRAGSTAFRLGGHGGGAAGSDAGSALGEEIPAHARPNV
                     AWSLRVAGICLVLWLGPVVVLLLALGADDVFSRIAVFFSKMAVVSFGGAYAVLAYVAQ
                     AAVETYGWLQPGEMLDGLGMAETTPGPLIMVVQFVGFMGAWREAGGLDPLLAATLAAV
                     LTTWVTFVPCFLWIFLGAPFIERLRDNKALSGALSAVTAAVVGVIANLALWFALHSLF
                     AEVRIWRGPGFSLDLPVPSSLDPAALVLTAAAVLAIFRFGLGMIWTLLGCVVAGMVWV
                     GLAR"
     gene            6463..6930
                     /locus_tag="P5558_02245"
     CDS             6463..6930
                     /locus_tag="P5558_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRY23162.1"
                     /translation="MSELSLPAIGGCRCGAVRFEITSAPLLTMACHCRGCQRMTGSAY
                     SLSAAIPKEGFRVTEGEPVIGGLHGANRHFFCGHCMSWLFTRLEGLDWFVNLRPTMLD
                     DPAWFEPFVETCTAEGLPWATTPAVYSFEGMPAMEDFDRVTRDYAEQRGQSVP"
     gene            6993..8453
                     /gene="gatB"
                     /locus_tag="P5558_02250"
     CDS             6993..8453
                     /gene="gatB"
                     /locus_tag="P5558_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRY23163.1"
                     /translation="MTYTIDGNTGTWEIVCGLEVHAQVTANAKLFSGAAAAFGAAPNS
                     QVSPVDAGMPGMLPVLNGYCVEQAVRTGLGLEAHINRLSIFERKNYFYPDLPNGYQIS
                     QYEHPIVGRGQLLIDMPDGHTRAIGITRLHLEMDAGKSMHDQRDGETLIDLNRSGVAL
                     MEIVSEPDIRTPEEAVLYLRKLRSILRYLGTCDGNMEEGSLRCDANVSVRRVGDEAYG
                     TRCEVKNVNSMRFVARAIEHEAKRQVELLEAGGKVVQETRLFDAVKGETRSMRGKEEA
                     HDYRYFPDPDLLPLVLPADFVDRLKATLPELPDIKKARFMAEYRLPAYDAGVLVAERA
                     TADYFEAVAQGRDGKQAANWIINELFGLLAKAGKGIDASPISTGHLGKLLDLLGTDAI
                     SGRIAKDVFAIMFETGDDPLAIVEAKGLKQVTDTAAIEAIVDEVIAGNPGQVETYLKN
                     PKVIGWFTGQVMKASGGKANPQAVGEILRAKLPPVE"
     gene            8489..9013
                     /locus_tag="P5558_02255"
     CDS             8489..9013
                     /locus_tag="P5558_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY23164.1"
                     /translation="MTGASGGGKSSIVEALGARGFTIVEEPGRAIVREQLATGGDATP
                     WQGPLKFCDLLFARSVAAYEALANRPEPVFFDRAMVDAIGWSDLIGAPVPKDWLAAIR
                     QLRYRTPVFVTPPWRAIYRNDAERRKDWPAVLADHNATVAAWRAGGYELIDVPRMPVA
                     ERVAFVLLHAGLGG"
     gene            9091..9300
                     /locus_tag="P5558_02260"
     CDS             9091..9300
                     /locus_tag="P5558_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRY23165.1"
                     /translation="MPRQISVTIDREDDGRWIAEIEALPGALAYGSTPEEARAKVEAL
                     ALRILADRREHGEPVPELDGLFEAA"
     gene            9297..9509
                     /locus_tag="P5558_02265"
     CDS             9297..9509
                     /locus_tag="P5558_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRY23166.1"
                     /translation="MSAFGSTRVRHVLTALLRLGWREKRRTGSHVVLERPGYADYVFA
                     FHDREEIGPRMLARIARRTGLRPEDL"
     gene            9510..9962
                     /locus_tag="P5558_02270"
     CDS             9510..9962
                     /locus_tag="P5558_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRY23167.1"
                     /translation="MTTLYLDADGCPVKDEAYRVAERYGLHTFVVANAWLRVPESAAI
                     ERVTVGDRLDEADDWIAERAGPGDIVVTSDIPLADRAIKAGARVVTPDGRVLDKRTIG
                     DVLATRNLMTDLREAGTIRGGGRPFGKAERSRFLEALDRVVVAISRGR"
     gene            complement(9946..10596)
                     /gene="nth"
                     /locus_tag="P5558_02275"
     CDS             complement(9946..10596)
                     /gene="nth"
                     /locus_tag="P5558_02275"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRY23168.1"
                     /translation="MLDPARIDDLIARLAAADPEQNRPPAAKMGRAPFESLMGVMLSA
                     QSRDAMTAKAKRQLLRRARTPEAILDLDETEIAALIKDCGLYNTKARNLKRMSAALLE
                     RHGGVVPRTREALMALPGVGRKCADIMLRFVFGEPEVPVDTHVFRVARRTGLAAGRTE
                     AAVAIELADRIPERWRWGAHMWLLNHGKRVCRARAPACDRCGIADLCERNGVSGPG"
     gene            10757..11968
                     /locus_tag="P5558_02280"
     CDS             10757..11968
                     /locus_tag="P5558_02280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY23169.1"
                     /translation="MALRIEPIRAALREPSFRAYLTANSVSLIGTWMQRIAVGWLAWE
                     LTESPFWLGAVAFADLAPAVLIAPYGGVLADRHDRRRILLWTQSAMAMVALAMALLLW
                     LDLVGIIGLTLLVALHGSLIGVNQPARLALVPALVGRSHLQTAIALNSVVFNSARFIG
                     PAVAGLVIAWSGVPAALLVNALTYLPILVVLPRLVLNENVRPGAWQGAARAFLEGIAH
                     VRGSAMLAPLFAFFIALSLTVRPLGELLPGFAGGIFDGGVEGLAVLSAAMGLGAVVGG
                     LWVALRADTFDLRRTLLLQLAVTAFATLFALSPGFVLAVPLIAGFGFTLTACGVAMHT
                     AVQLGVEPELRGRVMSLFGLVFRSVPALGALAVGTAAELLGLRVAMVLAAVVFVMVYA
                     WLWRGLPPIDR"
     gene            12113..12589
                     /gene="rplM"
                     /locus_tag="P5558_02285"
     CDS             12113..12589
                     /gene="rplM"
                     /locus_tag="P5558_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRY23170.1"
                     /translation="MKTYSAKPADIERDWFVIDAEGLVLGRLAVLVANRLRGKHKAMF
                     TPHMDCGDHIVVLNAEKVVLTGNKRTQKTYYRHTGYPGGIKETTAEKVLEGRFPSRVI
                     EKAVERMVPRGPLGRQVMRKLHIYAGGEHPHAGQSPKALDIAALNPKNSKLGTAHG"
     gene            12582..13067
                     /gene="rpsI"
                     /locus_tag="P5558_02290"
     CDS             12582..13067
                     /gene="rpsI"
                     /locus_tag="P5558_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRY23171.1"
                     /translation="MADTVSSFQDLSKLRPGIAAEPEVEVVKPKIDKFGRAYATGRRK
                     ESAARVWVKPGSGRFTVNGKEMPAYFARPTQQMVINQPLVSINRLQQYDIVCTVAGGG
                     LSGQAGAVRHGVARALIAYEPALRPALKHGGFLTRDAREVERKKYGKAKARRSFQFSK
                     R"
     gene            13271..14950
                     /locus_tag="P5558_02295"
     CDS             13271..14950
                     /locus_tag="P5558_02295"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY23172.1"
                     /translation="MRSSRFVRGALVALACLLATTPAAAQTAPTPVATNADPGPVWRY
                     TVRPGDNIWNLSRRYLADWRRWPELQALNGVEQPRSIPPGTRLSIPLAWLRLQPAAAM
                     LEGFQGSVALRRGGARPAPVQGMQLEVGDVVETGADSTAIIAFADGSRVLLGENAQLE
                     LDRLGEYRRTGMVDTRLKLERGRLETRVEPAVGSGSRFEVWTPPAVSSVRGTDLRVGL
                     DEAGERSATEVLTGNVRVAARSTARSVGAGMGTVTLQGSAPLPPRPLLDPHDIDLLPT
                     RLERLPLRMSIPPLEGAESFRLKVALDPGFEGILSEQVVPAGQPARLELAEGSYFARL
                     RGIEADGLEGRDQVWPLVVDARPEPPTPLGPKREGKVREPRPQFGWSVPLDAATVRLE
                     LARSADFGEPVIRAPDLTGATFTPAADLALGSWHWRLRTVDEAGEVGPWSDAWSFERV
                     DPPADPAIDALETESGRLTIRLASLEPGQKVRFQITDKTDFAELRHEALGDEAALVVP
                     GLPPGDYWFRAQIIEADGYMSEFSTPQRITVEPSRWWPVLLVPFALLFLLL"
     gene            14952..17792
                     /locus_tag="P5558_02300"
     CDS             14952..17792
                     /locus_tag="P5558_02300"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY23173.1"
                     /translation="MVTDAGIAFPRGLGFVVLATVLAALVVRLGPGDALDAVAYDVLQ
                     PWLAGPRSDRVAVVQIDQQSLERIGPWPWPRRVHARLIDRLAAAGPPVIGYDVLFAEP
                     SIGDPAGDLDLEEAVARAGNVVLPVVAEAPAAGQPPVEILPFGRLPELARALGHAHLR
                     PDADGRLRRLQLAAGVGDAFWPAFAVAVERQIRPRGSAPATFRGGRPPGSFLDWVEDG
                     EALVPVAADPRRLRRHSAADVLLGERLPSLADTAVLVGVTARGLAAPVRMGSGADTAP
                     PIAFQALAVEDLLQGRTVAPFRWPELAAAGLAAGAFLPWLFAAWRRPVLARSAFAMAP
                     LLPLLTGIGALLLSRLMLPFASVTVTLLGLLVVARLGEAARRVRRAQLERSLAHAALT
                     HVADAVLTTDDAGVVDYANPAAAKLFGRAAETLVGLPLAELAPPVAELARQTIEDPEP
                     PTPALLAAQLARPARGGAALGAIRLTAADGRQRSVRATARRFGSPAGSHLVLALNDIT
                     DEQRLLDEVAFRATHDSLTGLPNRHLLTDRLAGALDRARRAGNRVAVVFLDVDRLKSI
                     NDALGHAVGDAVLVEVASRFRLLGRSTDTVGRIGGDEFVLVLDGLESRTEIAAAVNRY
                     RGAFARPVVVGGRPLPVRASFGVAVFPDDGDSPDSLLRRADSAMYRAKRDGRDQVVTF
                     DAELHGETGDGLLLDAALRDAIARDGLELHYQPRIDLVERRPAGLESLVRWRHPDEGL
                     LPPARFIGLAEETGSIVELGRWVLERACADLAGPALRDRGLQLSVNVSVVQLKRDPGF
                     VDFVRGTLTRHGLAPQRIELEVTENLFLDPSLPMLGRRLASLAELGLHLSIDDFGTGY
                     SSLAYINRFPFDRIKIDRSFVDLIDRDQGAQAIVRAIVGLSTALAKQTTAEGVEKDAQ
                     LAFLDELSCNEAQGFFFGRPAPLPEMLRALPG"
     gene            17863..18777
                     /locus_tag="P5558_02305"
     CDS             17863..18777
                     /locus_tag="P5558_02305"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRY23174.1"
                     /translation="MLVILGSVNADLLFKVEKLPAPGETVLCPSYAMAPGGKGSNQAA
                     AAAKAGAATRFVGHLGDDAYGPVVRSLLVQAGIDTGGLARSKRPTAIAVIGVDATGEN
                     AIIVASGANLDTHAGQVEDALLGPSTTLLCQNEIRMTETAAVLARGKARGARTLLNLA
                     PAGPLPDGLLDTLDFLIVNEIEARMAAGDAAGEPLALARRLARAHDLVCVVTLGAEGA
                     IAVGPGGGWRVGSLRIEPVDTTGAGDAFVGVLAAALDQGRTLPEALHRASLAAGLSCL
                     AIGAQSAQPDAAMIESRLGELSAPTPLD"
     gene            18828..19532
                     /locus_tag="P5558_02310"
     CDS             18828..19532
                     /locus_tag="P5558_02310"
                     /inference="COORDINATES: protein motif:HMM:NF040005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HRY23175.1"
                     /translation="MQRRDVLRQGAGLVAGAALVGGIGPALAVPAGELRFAVFRNDRE
                     IGHHTLRFTPAGERLTVDIEIELEVRFAFITAYRYSHENREEWSNGQLAGFSSVTDDN
                     GTLHRVEASRQGGRVVIDGSEGQLTAPAGVLPATWWHADFIAGGRWINTQTGEIIAST
                     VTALGQETVEAEGRSVTAERFRLAGDVDMDLWYAGERWVKLAFNGPDESRIDYRKVEG
                     GAYSDLTYRPGEQRRA"
     gene            19541..21097
                     /locus_tag="P5558_02315"
     CDS             19541..21097
                     /locus_tag="P5558_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRY23176.1"
                     /translation="MARTLRFVFADQLTHELSSLADARAKHDLVLMAEVRAETDHVPH
                     HPKKIAFLFAAMRHFRDELRDRGLEVRYVELDDPDNRGSFEGELARALDELEIDRVVV
                     TEPGEYRVLERIKSWGETFDLPVQIRSDDRFLCSTAEFADWAGDRKQLRQEQFYRWMR
                     QRTGWLMTKGGKPVGGRWNYDAENRKPAAPGSDFPRPMHFAPDATTEAVLDVVRRRFG
                     SRFGETEPFWFAVTRGEARRALAHFVQTGLEGFGDHQDAMLEGEDWLCHSALSQYMNS
                     GLLTAREVGEAVLEAHRQRELPLNSVEGFIRQILGWREYVRGIYWLKMPGYAKLNGLG
                     NRRHLPAFYWTGETDMRCMAHALGQTRREALSHHIQRLMLTGNFALLAGVIPEEICTW
                     YLAVYADAFEWVELPNVLGMVMHADGGYLGSKPYAASGNYINRMSDFCGACTYDVKEK
                     LGPKACPFNYLYWSFLIEHREHLEENPRMGPMFRTLDRMSEERRERIMEDAGRFLDGL
                     EPWNPENDGD"
     gene            21087..21836
                     /locus_tag="P5558_02320"
     CDS             21087..21836
                     /locus_tag="P5558_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23177.1"
                     /translation="MVTERVWIIGASSGIGAALAERLAGDGKEVWASARSVGKLEALA
                     GKHPGYIHALPLDVTDKAAVQAALARIESACGPPDTVVLNAGTHQPVAAKDFTADGLR
                     QVMELNVFGVANGIEAVLPGMLERDAGRIAVVASVAGYQGLPTSAYYGASKAALINMI
                     ESLRFDLRRTGVVLQLVNPGFVRTPLTDRNEFPMPFLIEADEAAAAIARGLEVGRFEI
                     AFPTVFVWLLKLLGLLPYRAYFALVGRSTGK"
     gene            21833..22273
                     /locus_tag="P5558_02325"
     CDS             21833..22273
                     /locus_tag="P5558_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY23178.1"
                     /translation="MSVEAAAGVYARYFDELTTATTGDLRRLAAADMRFKDPFNDIRG
                     VDRVVGLFDHMFQTTEQPRFVTGAIAVTSRTAFLRWRFTCTVPSRLLSLALAIEGVSE
                     VRFDAAELVVEHVDYWDPAAAVYERLPLLGGVLRGLRRRLAHRP"
     gene            complement(22220..22801)
                     /locus_tag="P5558_02330"
     CDS             complement(22220..22801)
                     /locus_tag="P5558_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HRY23179.1"
                     /translation="MIRTVLAAVMLCVIAGCTAMKIDDFDGTAPAFVPEDYFLGETRA
                     WGLFQDRFGTVRREFVVDIEGRMEGDVLVLDEAFRYADGERDQRTWHIRPLGDGRYEG
                     EADDIVGTATGVREGKAMRWTYLFDLAVGDRIWRVSFDDWMFLQDETVMINRTTVSKW
                     GFTLGEVYIFFKKPELSGAVGETAAQASQDAAQ"
     gene            complement(22806..24095)
                     /locus_tag="P5558_02335"
     CDS             complement(22806..24095)
                     /locus_tag="P5558_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY23180.1"
                     /translation="MDLLAFGGLALPLAALNLPFYVYLPTFYVTELGLDLATVGAVLL
                     AARLLDVVTDPLVGTLGDRTRSRFGRRRPWMLGALPLLLVASFFLFLPREGAGGLYLF
                     LWASVAFLAWTMLLLAYSAWGAELSTDYDERSRIFGAREGFVILGIILAAALPTLIGA
                     GPGSATTMAAIFWSMALLLPLAFAVLLWRIGESAYAETRPLKFVAGLGVAFANRPFRR
                     LVFAYLLNGVANGLPATLFLLYVQHVIEAPGASGPLLLVYFASGLVAIPAWLWLSYRI
                     GKHRAWSLSMLWVCGVFALVPLLGAGDVWWFATVCVASGLGLGADLALPTAMQADVVD
                     ADSAVTGNRRAGLYFALWSMATKLSLALAVGIAFGTLDVAGFAAEGTSNGPIALLALV
                     GLYALLPVAMKFASIFLVWNFEIDAARLGELRAEGMP"
     gene            complement(24127..25371)
                     /locus_tag="P5558_02340"
     CDS             complement(24127..25371)
                     /locus_tag="P5558_02340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRY23181.1"
                     /translation="MTMGTDQGTSDTRATTGRAVDRVRLEAVLRVVRQLRRGTLDLTL
                     PDGSTETVRGAEAGPRAALAIRDAKVVDRYLRHGSVGFAEGYIAGEWDTPHLADLLEL
                     LDRNFDAWGQGYFGPVLTRFLARLQHWFRRNSRRGSQRNIHAHYDLGNEFFAAWLDPS
                     MTYSAALFDERHTAEKADLEAAQKAKYRHLCRMIELRPGQHLLEIGSGWGGFALTAAG
                     EFGAKVTSITISKAQHELASARVAEAGLREKVEIRFQDYRDVEGRFDRIASIEMFEAV
                     GEAYWPTYFEKVRSALVPGGMAGLQIITIADEHFESYRKGSDFIQQYIFPGGMLPSTT
                     ALAEHTGRVGLEITRKVAFGRDYARTLALWGERFETAWDGIRQLGFDERFRRVWRYYL
                     GYCEAGFRSGSTDVVQLALRRS"
     gene            complement(25413..26183)
                     /locus_tag="P5558_02345"
     CDS             complement(25413..26183)
                     /locus_tag="P5558_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRY23182.1"
                     /translation="MTGPALRSRLYEGTVVHQRLRPVKHRFRYRVFSLLLDLGELDAV
                     GSRLRLLGIERPGILGFRASDHGARDGSPLEPWALGHFATAGLDGVRRIELLCFPRLW
                     GFVFNPLSVYFGYDAEDRLMAVLYEVKNTFGGQHTYVLPTDPALAASGAWLRHAAAKT
                     FYVSPFIDMAAAYEFTLKPPGERLDLVIRETDRDGLFFTASQTGRALPLDDRNLALCL
                     LRNLAMTLKVIGGIHVEALRLWLKGAPFHPRTADPIDG"
     gene            complement(26180..27508)
                     /locus_tag="P5558_02350"
     CDS             complement(26180..27508)
                     /locus_tag="P5558_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY23183.1"
                     /translation="MHIGVVGSGIAGLSAAWLLAHRHRVTLFEKEQRLGGHSNTVTVS
                     AGERSVDVDTGFIVFNEPNYPELTSLLRHLGVATEASEMSFSASIAGGTLEYSGASPA
                     ALFAQKRNLVRPVFWGMLGDILRFNRLGREHLEERAKHAWSLGDFLRTHRFGEAFTGG
                     YLLPMAAAIWSCPTATMADFPATSFLRFFANHGLLTVTGQPKWRTLSGRSKSYVDALA
                     ARLEGRVRKAAPVVAVRRTNAGVEVVDGQGGSFRFDAVVMAAHAPQTAAVLLDADDEE
                     RRILGAFRYQPNRAVLHSDPALMPKRRRAWSAWNYTAPGLAEPGSRVAVTYWMNRLQN
                     IDESLPLFVSLNPLAEPDPALVHREFSYDHPVFDADAVAAQGAIGRIQGRGGIHYAGA
                     WLGYGFHEDGLRSGLEAAARLGVTAPWKRESRPMAGSGHGLPELAAAQSA"
     gene            complement(27764..28438)
                     /locus_tag="P5558_02355"
     CDS             complement(27764..28438)
                     /locus_tag="P5558_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23184.1"
                     /translation="MGSFLMFIVGIIVGVIAAWWFFERRDASSRATLEEGWKKKLAHV
                     EQEVRRADAAHDETKERLRLCQQEKVALEATPPAAAAPAPLVTEKAPAPAPAVPAEVE
                     RLAQARRRVDAKLAQLPAGSSARKRLMAERAALDGGEAKAAPAPNPVQLFTPPADAPD
                     DLEKIRGIGPVLRQKLNDLGITTFSQIAALTPADIERIDEMLDFKGRIEREDWIGQAR
                     ALGGQG"
     gene            complement(28551..30155)
                     /locus_tag="P5558_02360"
     CDS             complement(28551..30155)
                     /locus_tag="P5558_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY23185.1"
                     /translation="MNGFDYVVIGAGTAGCVLANRLSADPGTRVLLLEAGGRDNWFWI
                     HVPIGYLYTQNNPRTDWCFKTEAEPGLGGRALNYPRGRVLGGCSSINGMIYMRGQAAD
                     YDHWRQLGNPGWSWDDVLPWFKLSEDFCGGADELHGVGGEWRVEEPRVAWPILDAFRA
                     AAAEVGIPKTDDFNRGNNEGCGYFHVNQRRGVRWSAARAFLKPVRRRPNLIVVTGARV
                     SGLRLDGRRVTGVDYFENGLEKSVAAGREVILAAGSIGSPQLMLLAGIGPAGHLREQE
                     IEVRHALDGVGGNLQDHLQIRTVFKVENAVTLNEQAASLAGKARMALEYALHRRGPLS
                     MAPSTLGAFARTDPSFATPNVEYHVQPLSLGRFGEPLHPFPAITASVCNLRPTSRGTV
                     RLRSRDPAAPPAIAPSYLATEEDRKVAADAIRLTRTIMAAPALAPYAPEEHLPGPAVA
                     TEAELVQAAGALGTTIFHPVGTCRMGPAEDRTAVVDARLRVHGIGGLRVVDASIMPTI
                     TSGNTNSPVVMIAEKAARMIVEDARG"
     gene            30323..>32070
                     /locus_tag="P5558_02365"
     CDS             30323..>32070
                     /locus_tag="P5558_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRY23186.1"
                     /translation="MSRGRGVPPPLSATLGAVGRPERSWADLARFALPYVWPEGEPRL
                     RLRIVLAFACLVAAKILTVYVPFFLKWSVDALSVANLTAVPLALLIGYGAARLLASAF
                     QQLRDALFARVGARAGRRLALQVYTRLFDLSLRYHLERRTGELARAIDRGVKALSFLL
                     RTGLFSLGPTLLEFVMVIGVLLWNYPPSFALVTFVTIVGYAAFTILTTNWRTMIRREL
                     NRADNEFNASAVDGLINYEVVKAFGNEGFERRRLDGQLAAYETAAVRSEVSLAWLNGG
                     QAAILAVGVTILMVMAGRGVVGGSLTIGDVVLLNTFIWQLYQPLNFLGVIYREIRQSV
                     TDLEQIEALLELEPEVADAADAVPLIVTDGTVRFAEVRFAYDPRRPLVDGLDFTLEGG
                     RKLGIVGPTGAGKSTIVRLLFRFYDPDGGRVTIDGQDVSQVTQASLRGQIGLVPQDTV
                     LFNDTIAANIGYGRPGASMEAIEDAARLAQIHDFIESLPDGYRTTVGERGLKLSGGEK
                     QRVAIARVMLKNPPILILDEATSALDSTTERALQEALARAAKGRTTLVIAHRLSTITD
                     ADQIIVMDEGRVVERGT"
BASE COUNT         4637 a        11276 c        11236 g         4921 t
ORIGIN      
        1 gtcggatcga ccacccagag ccgctcgcgc tcgcccgcct cgcgccattt caggtcgcag
       61 cccgtgccca tcagcgtctt gaagatcttc gagaaatggg ccgagtgcag ggcgacgagg
      121 cccatgccgt ccagcacgcg ctggcgcacc cgctggacga tctcgtcggc gacctcgtca
      181 tgcgcccggt ggccccacca gacgagcacg tcggtcgcat ccaggcggcg gcggtcgagg
      241 ccgtgctccg gctcgtcgag cgtggccgtc gcgacctcga ggccgaccgc gcgcaacggg
      301 ccggccagcg cctcgtggat gccgcgcggg tagatctcgg cgactttcgg gttcgcctgc
      361 tcgtggcgga actcgttcca gacggtgacc ctcggctccc ggctcatttg ccgccctcct
      421 cggtcgcggt cgaatccatg atccccgccg cattggcgat ctcggccaag ggcaccagcc
      481 ggccgtgctc ggcggcggag cggatcgctg catgcatgag gcagaggctc tcgagattgt
      541 cggcgccggt gctcaggccc gcggcagggc ggccctcgcc cacccaggcg gcgaaggcgg
      601 cgagggcacc cgcccggtcg atgcgcggca agggctccag ctccagtggc cgcgacttgt
      661 cgtgggcggt gtagagggtc acccggtccg cacctcgccg gtcgccctgg ttgccgcgaa
      721 acctgccgag ggccgcggcg gcgctgccgt cgatccgcca gtccgcaccc cagggcgtgt
      781 ccggcccgcg gctgatccag gtgccgcgat agctgaccac ggtgccgcgc cgaaagcgga
      841 tcagggccgc cgccgccggc cggccctgga agggggtgcc gggctcgctc cagctcatcg
      901 ccgtcaccgc ctccggctcg tcgtcgagga cgtagcgcat catgtcgaaa tgatggatcg
      961 ccatgtccga gagcaggggc tcggcgagga agaagtagcg gtagttttcg tcccaggctt
     1021 tccagaaatc cacggcgata gaggcgacat ggccgagcgc accgtcggcc acgaggcggc
     1081 gaaaggcccg aggtgtgggg aaatgccgat agttctggtt gaccatgaga tgcagcttgg
     1141 tggccgccgc cagctcgacc agcgctgccg cctcgctcag ggtctcggtg aagggcttct
     1201 cgacgagcac gtgcaggccc cggcgaaggg cagcctcggt gaccgccgca tgggcggcga
     1261 gcggcaccac gacgagagcc gcggtcggcc cggtcgccgc caccgcctcg tcgagcgagg
     1321 cgaagagctt cgccggtgcg atgcggagcg cctcacgggc cagctcgcgc gtcgccgggt
     1381 cgccgtcgac gaaggccacc ggctcgacct ccggcaccgc cggcagcact tccctggccc
     1441 agtcccggcc ccagccgccg agcccgatct gcaccagccg caccatcgtc attctcctcg
     1501 ttcgaacgag cgtggtctag cagacgggcc ccgccgcgcg aagggcgcgc aacgcgtcct
     1561 cgaggatcac cgcggcggcg aagtggtcga tggcggcacc cggcttcggc cgcgccagcc
     1621 gcccctcggc gatcgcttgg cggaccgcct ccgtcgtcag ccgctcgtcc tggaggaagt
     1681 gcggcaggtc gagcgctcgt gccaggttct gcgccgtgtc cctggccgag cgggccctcg
     1741 gcccctcgct gccgtccatg ttgagcggca ggccgaggac cagcgccacc acgccgcgct
     1801 cgcggaccac ggccgagagg tgggccgtgt cagcggcgag ccctcggcgc tcgaacgtcg
     1861 aaagcgcggt caccaggcgg cgttccgggt cggtgccggc gagcccgatg cggcgcttgc
     1921 tcacatcgag ggcgagcagc gagccggtcg ccggcaggcc ggcggcgaag gctgccgccg
     1981 cgctcgtgct ggcctcgggg ctcaaggcag gagccggccg tagaagagga agggggcggt
     2041 cggcagggcc atgggcggtg cttccgcgct ctcgaccgag acgccgagcc cgagcccgtc
     2101 ggcctggtat tcctcgggca gctcgagcgt ggtcgtgcgg ccggcatcga gcgagccgag
     2161 cgcctgcggc gagccgtccg ggtctggcat ggcccagagc cgcaggaccc ggtcgtccgt
     2221 gagcaccaga ggcggcaccg gcgtcacgct cagctggttg ccggcgagcc cgacccgcca
     2281 gacccggccg ggggcgagcg gctgcaggag ccctacccga tcatgctcca gcgccaccgg
     2341 ggcgggtgct gcctccgccg gcgtgtcgac cgccggcggc aggagccggt cgccgagcag
     2401 gaccagcgcc agggccaggg ccactgaacc tgccgccacg gcacccaggc gccagcggcg
     2461 cagcgcccgg tcctgctcct cccgcatctc gttcggcatc gggtcgcggc cgcgcgccat
     2521 ggtggcgaca cccggcttga cgtggcggtc gaggcgcttg agcccgatgg cgatgtcgag
     2581 ccgctgccag acctcgggcg gcggctggac cggggcgacc gccgccttcg gcggcgtcgc
     2641 ccctgtcggc agatgccggt cgagcacggc ggcgagccag gcagccgggc gggccgcggt
     2701 gtcgcgttcc ttcgccgtgg cgaagatctc gaggaaggcg gcgaccagcg ccgcatcggc
     2761 ggccggccct tccactgccg cccgggcacg accatagagc tctggtgctg cggcgcggta
     2821 gagcgcctcc atcgcctgcc ggtcgtcggc gaccccggca tcgaggaggg tgccgagctc
     2881 ggcacccggc cgtttcgctg cgggcttgcg tttcgcggtc atgctgtccg atgccatttc
     2941 ctggggcccc atgccagcaa ggctcctcgc agcctcctag acattgcgac cggatttacg
     3001 caagtaggcg gcgctgccgg gccgatccat ccaacatggc tggcccggcc gccgttccga
     3061 tcaaccgtca ggataacgtt ccgatgtcgc tcgacaaggc caccgtgcgc aagatcagcg
     3121 agctcgcccg gatcgaggtg cccgaatccg acctcgagcc cttggcgcag gagctctcgg
     3181 cgatcatcgg ctggatcgag cagctggccg aggtcgacac cgacaacgtc gcaccgatgc
     3241 gctccgtcat gcccatcacc cacgcctggc gggccgacga ggtgaccgac ggcggcatgc
     3301 aggaggcgat cacgcaaaac gcacccagcg cccatgacgg ctatttcgtc gtgccgaagg
     3361 tggtcgagtg atggcggaca tgaccgatct cggcctcctc gaggcccgcc accggctcgc
     3421 ggcccgcgag ctctcggcca aagagctgac ggaggcgcat ctcgccgcca tgatcgaggc
     3481 ccgtccgctc aacgccttca taacagagac accggagctg gcgctggccg ccgccgaggc
     3541 gtccgatgcg cggctggcca ggggcgaggg cggcctgctc gagggcctgc cgatcgccat
     3601 caaggacctc ttctgcacga agggggtcca gaccaccgcc ggctcgcgca tcctcgaggg
     3661 cttcgtcccg ccttacgaat cgaccgtgac gcaaaggctc tgggccgagg gtgccgtgcc
     3721 gctcggcaag accaatctcg acgagttcgc catgggctcg tccaacgtga cctcgcattt
     3781 cggccccgtc cgcagtccct ggaccagggc cagcgacaat gccgacctcg tgccgggcgg
     3841 cagctccggc ggctcggccg cggccgtggc ggcccggata gcgcttgccg ccaccggcac
     3901 cgacacgggc ggctcgatcc gccagccggc ggccttctgc ggcatcaccg gcatcaagcc
     3961 gacctatggc cgctgctcgc gtttcggcgt ggtcgccttc gccagctcgc tcgaccagcc
     4021 cggcgccttc ggccgtagcg tcgaggacag tgcgctgctg ctgcaggcca tggcgggcta
     4081 cgatccgaac gacagcacct ccgccgacct cgccgtgccc gactggaccg cgagcctgaa
     4141 gggcaaggtg aaggggctca ggatcggcgt gccgaaggaa tatcgcgtcg acgcgatgcc
     4201 ggccgagatc gagaagctct ggcagcaggg tgccgactgg ctaagggccg aaggggcgga
     4261 gatcgtcgag atcgcgctgc cgcacaccaa gtacgcgctg cccacctact acatcatcgc
     4321 cccggccgag gcgtcctcca acctcgcccg ctatgacggc atgcgctacg gcctccgggt
     4381 cgagggcgag acgctcgccg atatgtacaa gaagacccgg gcggccggct tcggcacgga
     4441 agtgaagcgg cgggtcatga tcggcaccta cgtgctctcc gccggctact acgacgccta
     4501 ctacctcaag gcgcagaagg tccgccgccg catcgtcgag gacttcgagc aggccttcgg
     4561 aaaggtcgac gccatcctga caccgacggc cccgtccgcc gccttccgcg ccggcgagaa
     4621 gatggacgac cccgtcgcca tgtacctcaa cgacgtcttc accgtgccgt cgagcctcgc
     4681 cggcctgccg gcgatctcgg tcccggccgg cctgtcggcg gagggcctgc cgctggggct
     4741 gcaggtgatc ggcaaggcct gggacgagca gacggtgctc gatgtcgggc tggcgatcga
     4801 aagggcggcg gcgtttgcgg tgatgccgta tgggttgggg tgaagtcgct acacagcgta
     4861 agtgatcgac tgcgccaggc gcttccctga tgctttggaa caacccagcg aaccgggcca
     4921 atcccagcgg gcatcctatc cacaatgaaa atgacggatg gtcaaaaacc agctgacatg
     4981 aagtcagttg caaactgcat atgttgcacc aaagtgtccc gccccacggc cagctcccgc
     5041 ttgccctact gatgccgttg aacggccccg tccacgccat ccccttcccg gaggcgctgc
     5101 gcgtctggct gcgggtggcg ctcctgagct tcggcgggcc ggcggggcag atcgcggtga
     5161 tgcaccgcat cctcgtggag gagaagcgct gggtcgggga ggcgcggttc ctgcacgccc
     5221 tcaactactg catgctcctg ccgggtccgg aggcgcagca gctcgccgtc tatctcggct
     5281 ggctgctgca ccggaccaag ggcgggctgg tcgccggcac gctcttcgtc ctgcctggct
     5341 tcctctcgat cctggccctg agctgggtct acgtgctgct cggcgacctg acgctcgtcg
     5401 aggggctctt tttcgggctc aaggccgccg tcctcgccat cgtcctcgat gcgctggtgc
     5461 ggatcggccg gcgggcgctc aagaacaacg tgctggtcgg cacggcggtc gcagccttcg
     5521 tcgccatctt cgtcatcggc ctgccgttcc cgctcatcgt gctgggggcg gcgctcgccg
     5581 gctttgtcgc cacgcgcgcc ggctcgaccg cctttcgcct cggcggccat ggcggcggtg
     5641 cggccgggag cgacgcggga tcggcgctgg gcgaggagat cccggcccat gcccggccca
     5701 acgtcgcctg gtcgctgcgg gtcgccggca tctgcctcgt cctctggctc gggccggtcg
     5761 tcgtgctgct gctggcgctc ggcgcggatg acgtcttcag ccggatcgct gtcttcttca
     5821 gcaagatggc ggtcgtctcc ttcggcggcg cctacgcggt gctggcctat gtcgcccagg
     5881 cggccgtcga gacctatggc tggctccagc cgggcgagat gctggacggg ctaggcatgg
     5941 ccgagacgac gccggggccg ctcatcatgg tcgtccagtt cgtcggcttc atgggtgcct
     6001 ggcgcgaggc cggcggtctc gatccgctgc tcgccgccac gctggccgcc gtgctcacca
     6061 cctgggtcac cttcgtgccc tgcttcctct ggatcttcct gggtgctccc ttcatcgagc
     6121 ggctgcgcga caacaaggcg ctcagcgggg cgctttccgc cgtcaccgcc gccgtggtcg
     6181 gggtcatcgc caacctcgcc ttgtggttcg cgctgcacag cctcttcgcc gaggtccgca
     6241 tctggcgggg gccgggcttc tcgctcgacc tgcccgtccc gtcctcgctc gatcccgccg
     6301 cgctggtgct cacggcagcc gcggtcctcg cgatcttccg cttcgggctc ggcatgatct
     6361 ggacgctgct gggctgcgtc gtcgccggca tggtctgggt ggggctggcc cggtgaggcc
     6421 ggcgctacca tctgcagaag ggcaattcgg gaggagggac cgatgagcga actgagcctg
     6481 ccggcgatcg gcggctgccg ctgtggtgct gtccggttcg agatcacgtc ggcaccgctc
     6541 ttgaccatgg cctgccactg ccggggctgc cagcgcatga ccggcagcgc ctattcgctg
     6601 agtgcggcga tcccgaaaga gggattccgg gtgacggaag gcgagcccgt cattggcggc
     6661 ctgcacggcg ccaaccgcca tttcttctgc ggccactgca tgagctggct cttcacccgc
     6721 ctcgaggggc tggactggtt cgtcaacctg cgcccgacca tgctggacga ccccgcctgg
     6781 ttcgagcctt tcgtcgagac ctgcacggcc gaggggttgc cctgggcgac gacgcccgcg
     6841 gtctacagct tcgagggcat gccggcgatg gaggacttcg atcgggtgac gcgggactac
     6901 gcggagcagc gcggccagtc ggtgccctga tctccttgtc cggacccggg aaggaccgct
     6961 agacggggcg gcgcagcgac gaggatcgat ccatgaccta caccatcgac ggcaacactg
     7021 gcacctggga gatcgtctgc gggctcgagg tgcatgccca ggtgaccgcc aacgccaagc
     7081 tcttctccgg cgccgcggcg gcgttcgggg cggcccccaa cagccaggtg agcccggtcg
     7141 atgccggcat gccgggcatg ctgccggtgc tcaacggcta ctgcgtcgag caggcggtcc
     7201 ggaccgggct cgggctcgag gcgcatatca accggctctc gatcttcgag cggaagaact
     7261 atttctaccc ggacctgccg aacggctacc agatcagcca gtacgagcat ccgatcgtcg
     7321 gccgcggcca gctcctgatc gacatgccgg acgggcatac gcgcgccatc ggcatcaccc
     7381 gcctgcatct cgagatggat gccggcaagt cgatgcacga ccagcgcgac ggcgaaaccc
     7441 tgatcgacct caaccgctcc ggcgtggcgc tcatggagat cgtctccgag cccgacatcc
     7501 gcacgcccga ggaggccgtg ctctacttgc ggaagctgcg ctcgatcctg cgctatctcg
     7561 gcacctgcga cggcaacatg gaggagggct cgctgcgctg cgacgccaat gtctcggtcc
     7621 gccgcgtggg cgacgaggcc tatggcacgc gctgcgaggt caagaacgtc aattcaatgc
     7681 gcttcgtcgc cagggcgatc gagcacgagg cgaagcgcca ggtcgagctg ctcgaggccg
     7741 gcggcaaggt ggtccaggag acccggctct tcgatgcggt gaagggcgag acgcgctcga
     7801 tgcgcggcaa ggaggaggcg cacgactatc gctacttccc cgatcccgac ctcctgccgc
     7861 tggtcctgcc ggcggatttc gtcgaccgcc tcaaggcgac cctgcccgag ctgccggaca
     7921 tcaagaaggc gcgcttcatg gccgaatacc gcctgccggc ctacgatgcc ggcgtgctcg
     7981 tcgccgagcg cgccaccgcc gactacttcg aggcggtggc ccagggccgc gacggcaagc
     8041 aggcggccaa ctggatcatc aacgagctct tcggcctgct cgccaaggca ggcaagggca
     8101 tcgacgcgag cccgatcagc acaggccacc tcggcaagct gctcgatctc ctcggcaccg
     8161 atgcgatctc cgggcggatc gccaaggacg tcttcgccat catgttcgag accggcgacg
     8221 accccctggc catcgtcgag gccaagggcc tgaagcaggt gaccgacacg gccgcgatcg
     8281 aggccatcgt cgacgaggtg atcgccggca atcctggcca ggtcgagacc tatctcaaga
     8341 acccgaaggt gatcggctgg ttcaccgggc aggtgatgaa ggcctcgggc ggcaaggcca
     8401 acccgcaagc agtgggcgag atcctgcgcg cgaagctgcc gcccgtggag tagctgccgc
     8461 gccggccgac cgccccgatt tctttgtcct gaccggcgcc tcgggcggcg gcaagtcgtc
     8521 gattgtcgag gcgctgggcg cacgcggctt caccattgtc gaggagcccg ggcgcgcaat
     8581 cgtccgtgag cagctggcga ccggcggcga tgccacgccc tggcagggcc ctttgaagtt
     8641 ctgcgacctg ctcttcgccc gctcggtggc ggcgtacgag gcgctggcca accgtccgga
     8701 gccggtcttc ttcgatcgcg ccatggtcga cgcgatcggc tggtcggatc tgatcggagc
     8761 tccggttccg aaggactggc tcgccgcgat caggcaattg cgctaccgca cgcccgtctt
     8821 cgtcaccccg ccctggcggg cgatctaccg caacgacgcc gagcgccgca aggactggcc
     8881 ggcggtcctg gccgaccaca acgccaccgt cgccgcctgg cgggcgggcg gctacgagct
     8941 gatcgacgtg ccgaggatgc cggtggccga gcgcgtcgcc ttcgtccttc tccatgccgg
     9001 gctcgggggt tgatgcaccc gtacaccccg atgataccca ggatgcgggg acgcgtgcta
     9061 ggatcgaggc gcgtagacgg tggagcgacg atgccgagac agatcagcgt gacgatcgac
     9121 cgcgaggatg acgggcgctg gatcgccgag atcgaggcgc tgcccggtgc cttggcgtat
     9181 ggttcgacac cggaggaagc gcgggcgaag gtcgaggcgc tggcgctgcg gattctcgcc
     9241 gatcgccgtg agcacggcga gccggtgccc gagctcgacg gcctcttcga ggcagcttga
     9301 gcgccttcgg ctcgacccgt gtccggcatg tgctgacagc gctgctgcgg ctcggctggc
     9361 gagagaagcg gcgcacggga tcgcatgtcg tcctcgagcg gcccggctac gccgattacg
     9421 tcttcgcctt tcacgaccgc gaggaaatcg gtccgcgcat gctcgcccga attgccaggc
     9481 gcaccggcct gcgtcctgag gatctctgag tgaccaccct ctatctcgac gccgacggct
     9541 gcccggtgaa ggacgaggcc tatcgcgtgg ccgagcgcta cgggctgcac accttcgtcg
     9601 tcgccaatgc gtggctcagg gtgcccgaga gcgcggcgat cgagcgggtc accgtcggcg
     9661 accggctgga cgaggcggac gactggatcg ccgagcgggc ggggcccggc gacatcgtcg
     9721 tcaccagcga catcccgctc gccgaccggg cgatcaaggc cggagcacgg gtcgtgacgc
     9781 cggacggccg ggtgctggac aagcggacca tcggcgacgt gctcgccacc cgcaacctca
     9841 tgaccgacct gcgcgaggcc ggcaccatcc ggggtggcgg ccggcccttc ggcaaggccg
     9901 agcgttcgcg cttcctcgag gcgctcgacc gggtggtcgt ggcgatcagc cggggccgct
     9961 gaccccgttt cgctcgcaga gatcggcgat gccgcaccgg tcgcaggccg gggcgcgggc
    10021 gcggcagacc cgcttgccat ggttcaggag ccacatgtgc gcgccccagc gccatcgctc
    10081 cgggatccgg tccgccagct cgatcgccac cgccgcctcg gtccgcccgg cggcgaggcc
    10141 ggtccgacgt gccacccgga agacgtgcgt gtcgaccggc acctcgggct cgccgaagac
    10201 gaagcgcagc atgatgtccg cgcatttgcg accgacgccc ggcagcgcca tgagcgcctc
    10261 gcgcgtgcgc ggcacgacgc cgccatgccg ttcgagcagc gcagcactca tccgcttcag
    10321 gttgcgcgcc ttggtgttgt agaggccgca atccttgatg agggccgcga tctccgtctc
    10381 gtcgaggtcg aggatcgcct cgggcgttcg tgcgcggcgc agcagctgcc gcttggcctt
    10441 cgcggtcatc gcatcgcgcg attgcgcact gagcatgaca cccatcaggc tctcgaaggg
    10501 agcgcgcccc atcttcgcag cgggcggccg gttctgctcc gggtcggctg ccgcgaggcg
    10561 ggcgatgagg tcgtcgatcc gggccgggtc gagcatgagg tggcgtgatc catcaaccgc
    10621 ttggtcaggc cagatagggc attcgccggc cgggccaagc atcgccgcat caatcccttt
    10681 ccctgtcggg tccggccggc tacccaaagc gtcgcgacag cggtttcacc gctgcgaccc
    10741 tcggaggccg gtccccttgg ccctgcgcat cgaacccatc cgggcagcgc ttcgcgagcc
    10801 ctcgttccga gcctatctca cggccaattc ggtctcgctg atcggcacct ggatgcagcg
    10861 catagccgtc ggctggctcg cctgggagct caccgaatca cccttctggc tcggtgccgt
    10921 cgccttcgcc gatctggcgc cggcggtgct catcgccccc tatggcgggg tgctggccga
    10981 tcgtcacgac cggcgccgga tcctgctctg gacccagagc gccatggcca tggtcgccct
    11041 cgccatggcg ctgctgctct ggctggatct cgtcggcatc atcgggctga cgctgctggt
    11101 ggccctgcat ggcagcctga tcggcgtcaa ccagccggcc cgcctggcgc tcgtcccggc
    11161 cctggtcggc cggtcgcacc tgcagacggc gatcgcgctc aacagcgtcg tcttcaattc
    11221 ggcgcggttc attggcccgg cggtcgccgg gctggtgatc gcctggtcgg gggtgccggc
    11281 ggcgctgctc gtcaacgcgc tcacctacct gcccatcctc gtcgtcctgc cccgcctcgt
    11341 gctcaacgag aacgtgcggc cgggtgcgtg gcagggggcc gcgcgcgcct ttctcgaagg
    11401 catcgcgcat gtccgtggca gtgcgatgct ggcgccactc ttcgcgtttt tcatcgctct
    11461 ctcgctcact gtccggccac tcggcgagct gcttcccggc ttcgccggcg gcatcttcga
    11521 cgggggtgtc gaggggctcg cggtgctcag cgccgccatg ggcctgggtg ccgtggtggg
    11581 cggcctctgg gtggcactgc gcgccgacac cttcgatctg cgccgcacgc tgctgctgca
    11641 gctcgccgtc accgcctttg ccaccctctt cgcgctgagc cccggcttcg tgctggcggt
    11701 gccgctcatc gcgggcttcg gcttcaccct gaccgcctgc ggcgtggcga tgcacacggc
    11761 cgtgcagctg ggggtcgagc cggagctccg ggggcgggtg atgagcctct tcggcctcgt
    11821 tttccgcagc gttccggcgc tgggtgccct ggccgtcggc acggccgccg agctcctcgg
    11881 cctcagggtc gccatggtgc tggcggccgt ggttttcgtc atggtctacg cctggctctg
    11941 gcgcggcctg ccgccgattg accgttgaca ggcacggctc tcctcaccta gatggcgccg
    12001 ttgtcggcgg gtgcccccgg tgctcgctcg atctctgttg tccgacagct tcggccgtcc
    12061 gcccgccagg gggctcgttc ccagggcgtg gcgcagcctc gtgagcagct tcatgaagac
    12121 ctattccgcc aagcccgccg acatcgaacg ggactggttc gtgatcgacg ccgaagggct
    12181 ggtcctcggc cgcctcgccg tgctcgtcgc caaccggctg cgcggcaagc acaaggcgat
    12241 gttcacgccg cacatggact gcggcgacca catcgtcgtg ctgaacgccg agaaggtcgt
    12301 cctgaccggc aacaagcgca cgcagaagac ctattaccgc cacaccggct atccgggcgg
    12361 catcaaggag accaccgccg agaaggtgct cgagggccgc ttcccctccc gggtcatcga
    12421 gaaggcggtg gagcgcatgg tgccgcgcgg cccgctcggc cggcaggtca tgcgcaagct
    12481 ccacatctac gccggcggcg agcatccgca tgcgggccag agcccgaagg cgctcgacat
    12541 cgccgccctg aacccgaaga acagcaagct cggaaccgcg catggctgac accgtctcct
    12601 ccttccagga cctctcgaag ctgcggcccg gcatcgccgc cgagccggag gtcgaggtcg
    12661 tgaagccgaa gatcgacaag ttcggccgcg cctatgccac cgggcggcgc aaggagtcgg
    12721 ctgcacgggt ctgggtcaag cccggcagcg gccgcttcac ggtcaacggc aaggagatgc
    12781 cggcctattt cgcccggccg acgcagcaga tggtgatcaa ccagccgctg gtgagcatca
    12841 accggctgca gcagtacgac atcgtctgca cggtggccgg cggcggcctc tcgggtcagg
    12901 ccggtgccgt gcggcacggc gtggccaggg cgctgatcgc ctacgagccg gcgcttcgcc
    12961 cggccctcaa gcatggcggg ttcctgaccc gcgacgcgcg cgaggtcgag cgcaagaagt
    13021 acggcaaagc caaggcgcgg cgcagcttcc agttctcgaa gcgctgagcc gacccgaacc
    13081 agccgcaccc gaacaaaagg gcgccggact tgctccggcg ccctttttcg tgcccgaaga
    13141 gatcccgact aatcgagggt agaaaagagc tccgttcctc gtcatcttga ggcgaacaag
    13201 cgaggccggc gcgcaaacgg cctgctctca acgattgatc gggtggactt tgtcgattca
    13261 ggcggtctgc gtgagatcat cccggttcgt gcgcggcgct ctcgtcgcgc tcgcctgtct
    13321 tctcgccaca acgccggcgg ccgcccagac agcaccgacg ccggtcgcga cgaacgccga
    13381 tcccggcccg gtctggcggt acaccgtgcg gccgggcgac aatatctgga acctgtcgcg
    13441 tcgctacctg gccgactggc ggcgctggcc ggagctacag gcgctcaacg gggtcgagca
    13501 gccgcgctcg atcccgcccg gcacccgtct ttccattccg ctcgcctggc tgcgcctgca
    13561 gccggccgcg gcgatgctcg agggttttca ggggagcgtc gcgctccgtc gcggtggggc
    13621 acgaccggcc cccgtccagg gcatgcagct cgaggtgggc gatgtggtcg agaccggcgc
    13681 tgattccacg gccatcatcg ccttcgctga cggttcgcgc gtgctgctcg gcgaaaacgc
    13741 ccaactggag ctggaccggc tcggcgagta tcgccgcacc ggcatggtcg acacgcgcct
    13801 caagctcgag cgcggccgcc tcgaaacccg ggtcgagccg gcggtcggct cgggctcccg
    13861 cttcgaggtg tggacgccgc cggcggtcag ctcggtgcgc ggcaccgatc tccgggtcgg
    13921 tctcgacgag gcgggggagc gcagtgctac ggaagtgctc accggcaacg tccgggtcgc
    13981 ggcccggtcg accgcccgca gcgtcggggc cggcatgggg acggtgacgc tgcagggttc
    14041 ggcacccttg ccgccgcggc cgctgctcga tccgcacgac atcgacctcc tgccgacccg
    14101 tctcgaacgc ctgccgctcc gcatgtcgat cccgccgctg gagggtgccg agagctttcg
    14161 cctcaaggtc gcgctggacc cgggcttcga agggattttg agcgagcagg tcgtgccggc
    14221 cggccagccg gcgcgattgg agctcgccga aggcagctat tttgcccggc tgcgcggcat
    14281 cgaggccgat gggctggaag gcagggacca ggtctggccg ctcgtggtcg acgcccggcc
    14341 cgagccgccg acgccgctgg gcccgaagcg cgagggcaag gtgcgcgagc cgcggccgca
    14401 attcggctgg tcggtgccgc tcgacgccgc caccgttcgg ctcgagctgg cgcggagcgc
    14461 ggactttggc gagcccgtga tccgtgcccc ggacctgacc ggggcgacct tcacgcccgc
    14521 ggccgatctg gcgctcggct cctggcactg gcgcctgcga acggtcgacg aggcgggcga
    14581 ggtggggccg tggagcgacg cctggagctt cgagcgggtc gatccgccgg ccgatccggc
    14641 gatcgacgcc ctcgagaccg agagcggccg gctgaccatc cgcctcgcct cgctcgagcc
    14701 gggccagaag gtccgcttcc agatcaccga caagaccgac ttcgccgagc tgcgccacga
    14761 ggcgctcggc gacgaggcag cactggtggt gccggggctg ccccccggcg actactggtt
    14821 ccgcgcccag atcatcgagg cggacggcta catgtcggaa ttctcgaccc cgcaacgcat
    14881 cacggtggag ccgagccgct ggtggccggt gctgctcgtg cccttcgcgc tgctgttcct
    14941 gctgctctga gatggtgact gacgcgggca tagcgtttcc ccgcggcctg gggttcgtcg
    15001 tcctcgcgac ggtgctcgcc gcgttggtcg tccgcctcgg gccgggcgat gcgctggatg
    15061 ccgtggccta tgacgtgctg cagccctggc tcgctggtcc gcgcagcgac cgtgtcgcgg
    15121 tcgtccagat cgaccagcag agcctcgagc ggatcggccc ctggccctgg ccgcggcggg
    15181 tccatgcccg gctgatcgat cggctcgccg ccgccggacc cccggtcatc ggctatgacg
    15241 tgctctttgc cgagccctcg atcggcgacc cggcgggcga tctggacctc gaggaggcgg
    15301 tggcgcgtgc cggcaatgtc gtcctgccgg tggtggccga agcgccggcc gccgggcagc
    15361 cgccggtcga gatcttgccg ttcggtcgcc tgccggagct ggcccgcgcc ctcggccacg
    15421 cccatctccg gccggacgcg gacggccggc tgcgccggct gcagctcgcc gccggggtcg
    15481 gcgacgcctt ctggccggcc ttcgcggtgg cggtggagcg ccagatccgg ccgcgaggct
    15541 cggcgcctgc cacctttcgt ggcgggcggc cgcccggcag cttcctcgac tgggtcgagg
    15601 atggcgaggc gctcgtgccg gtcgccgccg atccccggcg gttgcgccgc cattccgccg
    15661 ccgacgtgct cctgggcgag cgcctgccgt cgctcgccga caccgctgtg ctggtcgggg
    15721 tgacggcgcg cgggctcgcc gctcccgtcc ggatgggctc gggtgccgac actgctccgc
    15781 cgatcgcctt ccaggcgctg gcagtcgagg acctgctgca gggccggacc gtcgccccgt
    15841 tccgctggcc cgagctggcg gccgccggcc tcgccgccgg tgcgttcctg ccgtggctct
    15901 tcgccgcctg gcgccggccg gtgctggcgc gctccgcctt cgccatggcg ccgctgctgc
    15961 cactgctcac cgggatcggc gccctcttgc tgtcgcgcct gatgctgccc ttcgcctccg
    16021 tcacggtgac gcttctaggc ctgctggtcg tggcccggct gggcgaggcg gcacggcgcg
    16081 tccgccgggc gcagctcgag cgcagcctcg ctcacgctgc cctgacccac gtggcggatg
    16141 cggtgctgac caccgacgac gccggcgtgg tcgactatgc caacccggca gcggcgaagc
    16201 tcttcggccg cgccgccgag acgctggtcg gcctgccgct cgccgagctg gcgccgccgg
    16261 tcgccgagct cgcccggcag acgatcgagg acccggagcc accgacaccg gcgctgctcg
    16321 ccgcccagtt ggcccgaccg gcgcgtggcg gcgcggcgct cggcgcgatc cgcctgacgg
    16381 ccgcggacgg gcgccagcgc tcggtgcgcg ccaccgcccg ccgcttcggg tcgcccgctg
    16441 gctcgcatct ggtcctggca ctgaacgaca tcacggacga gcagcggctg ctcgacgagg
    16501 tggcgttccg ggcaacccac gacagcctga ccgggctgcc caaccggcat ctcctgaccg
    16561 atcgtctggc gggcgccctc gaccgggcac ggcgtgccgg caaccgcgtg gccgtcgtgt
    16621 tcctcgatgt cgatcgcctg aagagcatca acgacgcgct cggccacgcg gtcggcgatg
    16681 ccgtgctggt cgaggttgcc tcgcgctttc gcctgctcgg ccgctcgacc gacacggtcg
    16741 ggcggatcgg cggcgacgag ttcgtgttgg tgctggatgg cctcgagagc cgcaccgaga
    16801 tcgccgcggc cgtcaatcgc taccgcggcg ccttcgctcg tccggtcgtc gtcggcggcc
    16861 gccccctgcc ggtccgggcg agctttggtg tcgcggtctt tccggacgat ggtgactccc
    16921 cggacagcct gctccggcga gcggattccg ccatgtaccg ggcgaagcgg gacggtcgcg
    16981 accaggtggt caccttcgat gccgagctgc acggcgagac aggcgacggt ctcctgctcg
    17041 atgcagcact gcgcgacgcc atcgccaggg acgggctcga gctgcactac cagccgcgca
    17101 tcgacctcgt cgagcgccgg ccggccggac tcgaatcgct ggttcgctgg cgccatccgg
    17161 acgagggcct cctgccgccg gcccgcttca tcgggctcgc cgaggagacc ggctcgatcg
    17221 tcgagctcgg ccggtgggtc ctagagcggg cctgcgccga tctcgccggg ccggcgctgc
    17281 gcgatcgcgg cctgcagctg tcggtcaacg tttcggtcgt gcagctcaag cgcgaccccg
    17341 gcttcgtcga cttcgtccgc ggcaccctga cgcggcacgg cctggcgccg cagcggatcg
    17401 agctcgaggt caccgaaaac ctcttcctcg acccgtccct gccgatgctg gggcgccggc
    17461 tcgccagcct cgccgagctc ggcctgcacc tctcgatcga cgatttcggc acgggctatt
    17521 cctcgctcgc ctatatcaac cgcttcccct tcgaccggat caagatcgac cgctccttcg
    17581 tcgacctgat cgaccgggac cagggtgcgc aggcgatcgt ccgcgccatc gtcgggctga
    17641 gcacggcgct ggccaagcag accaccgccg agggggtcga gaaggatgcc cagctcgcct
    17701 tcctcgacga gctgagctgc aacgaggccc agggcttctt cttcggccgc ccggcacccc
    17761 tgcccgagat gctgcgggcg ctgccgggct gagccgggcg gctggacagg ccggttcgca
    17821 ggccgcatat cggccggaat gctgtgatcg gggagggcgg cgatgctggt gatcctgggc
    17881 tcggtgaatg ccgatctcct gttcaaggtc gagaagctgc cggcgccggg cgagaccgtg
    17941 ctctgcccgt cctatgccat ggcgccgggc ggcaagggct cgaaccaggc cgcggccgcc
    18001 gccaaggccg gtgcggcaac gcgattcgtc ggccatctcg gcgacgacgc ctatggaccg
    18061 gtggtccgca gcctcctggt ccaggccggc atcgacacgg gtggcctcgc caggtcgaag
    18121 cgcccgaccg cgatcgcggt gatcggcgtc gacgcgacgg gcgagaacgc gatcatcgtc
    18181 gccagcggcg ccaatctcga tacccatgcc ggccaggtgg aggacgcctt gctcggcccc
    18241 tcgaccacgc tcctttgcca gaacgagatc cggatgaccg agaccgccgc ggttctggcc
    18301 cgcggcaagg ccaggggtgc ccggacgctc ctcaacctcg caccggccgg ccccctgccg
    18361 gacggtttgc tggacacgct cgacttcctc atagtcaacg agatcgaggc gcgcatggcc
    18421 gccggcgacg cggcggggga gccgctcgct ttggcccgcc gcctggccag ggcgcacgac
    18481 ctcgtctgcg tcgtgacgct gggcgcggag ggtgcgatcg cggtcggccc agggggcggc
    18541 tggcgggtcg gcagcctcag gatcgagccg gtcgacacga cgggagccgg cgacgccttc
    18601 gtcggcgtgc tcgccgccgc actcgaccag gggcgaacgc tgcccgaggc gctgcaccgg
    18661 gcgagcctcg ccgccgggct ttcctgcctc gccatcggcg cccagtcggc ccagccggat
    18721 gcagcgatga tcgaatcacg tctgggtgag ctgtcggcgc cgacgccgct cgactgatcc
    18781 cgggaggcgc ctcgcgcgta tcttcggcga gcagcgggag agaggcgatg cagcggcgtg
    18841 acgtattgcg acagggggca gggctggtgg ccggggcggc ccttgtcggc ggaatcggac
    18901 cggccctggc ggtgccggcg ggcgagttgc gcttcgccgt cttccgcaac gaccgcgaga
    18961 tcgggcatca cacgctccgc ttcacgccag cgggcgagcg tctgaccgtc gacatcgaga
    19021 tcgagctcga ggtccgcttc gccttcatca ccgcctaccg ctacagccac gagaaccgcg
    19081 aggaatggtc gaatggccag ctcgccggct tctccagcgt caccgacgac aacggcacgc
    19141 tgcatcgggt cgaggcgtcg cgccagggcg gtcgtgtggt catcgacggg agcgaaggac
    19201 agctcaccgc cccggccggc gtgctccccg ccacctggtg gcacgccgac ttcatcgccg
    19261 gagggcgctg gatcaacact cagacgggcg agatcatcgc ctcgaccgtg acagcgctcg
    19321 ggcaggagac ggtagaggcg gaaggtcgat ccgtcacggc cgagcggttt cgcctcgcgg
    19381 gggatgtcga catggatctc tggtacgcgg gcgagcgctg ggtgaagctc gccttcaacg
    19441 ggccggacga gtcgcggatc gactatcgca aggtcgaggg tggcgcctac agcgacctca
    19501 cctatcggcc cggcgagcag cgccgcgcct gagcggagcc atggcccgaa cgcttcgctt
    19561 cgtcttcgcc gaccagctca cccacgagct ttcctcgctc gccgatgcac gggcgaagca
    19621 tgatctggtt ctcatggccg aggtccgggc cgagaccgac cacgtcccgc atcacccgaa
    19681 gaagatcgcc ttcctgttcg ccgccatgcg ccatttccgg gacgagctgc gggaccgggg
    19741 cctggaggtt cgctatgtcg agctggacga tcccgacaac cgcggcagct tcgagggcga
    19801 gctcgcccgc gcgctcgacg agctcgagat cgaccgcgtc gtcgtcaccg aacccggcga
    19861 gtatcgggtg ctggagcgga tcaagagctg gggcgagacg ttcgacctgc cggtccagat
    19921 ccggagtgac gaccgttttc tctgctccac cgccgaattc gccgactggg cgggggaccg
    19981 gaagcagctc cgccaggagc agttctaccg ctggatgagg cagcgcaccg gctggctgat
    20041 gacgaagggg ggcaagcccg tcggcggacg ctggaactac gacgcggaga acaggaagcc
    20101 ggcggcgccg ggctccgact tcccgcggcc aatgcatttt gccccggatg ccaccacgga
    20161 ggccgtgctc gacgtcgtcc gccgccgttt cggtagccgc ttcggtgaga ccgagccgtt
    20221 ctggttcgcc gtcacgcgtg gcgaggcgcg gcgggcgctc gcccatttcg tgcagaccgg
    20281 gctcgagggc ttcggcgacc atcaggacgc gatgctcgag ggcgaggact ggctctgtca
    20341 ttcggcgctc tcgcagtaca tgaactctgg cctgctcacc gcccgagagg tcggcgaggc
    20401 ggtgctggag gcgcacaggc agcgcgagct gccgctcaac agcgtcgagg gcttcattcg
    20461 ccagatcctc ggctggcgcg aatacgtgcg cggcatctac tggctgaaga tgccgggcta
    20521 cgcgaagctg aacgggctcg ggaaccggcg ccacctgccg gccttctact ggaccggcga
    20581 gaccgacatg cggtgcatgg cgcacgccct gggccagacc aggcgggagg ccctgtcgca
    20641 ccacatccag cggctcatgc tgaccggcaa tttcgccctg ctcgccgggg tgatcccgga
    20701 ggagatctgc acctggtatc tcgccgtcta tgccgacgcc ttcgagtggg tggagctgcc
    20761 caacgtgctc ggcatggtga tgcatgccga cggcggctat ctcggctcga agccctatgc
    20821 cgcctccggc aactacatca accggatgtc ggacttctgc ggcgcctgca cctacgacgt
    20881 gaaggagaag ctcgggccga aggcctgccc cttcaattac ctctactgga gcttcctcat
    20941 cgagcaccgt gagcacctgg aagagaatcc gcgcatgggc cccatgttca ggacgctcga
    21001 ccgcatgtcg gaggaacgca gggagcggat catggaagac gctggccggt ttctcgatgg
    21061 cctcgagcct tggaacccgg agaacgatgg tgactgagcg cgtctggatc atcggggcta
    21121 gctctggcat aggggcggcg ctcgccgagc gtcttgccgg cgacggcaag gaggtctggg
    21181 ccagcgcccg gagcgtcggc aagctcgagg cgctggccgg gaagcatccg ggctacatcc
    21241 acgccctgcc gctcgacgtc accgacaagg cggctgtcca ggcagcgctc gccaggatcg
    21301 aatctgcctg cggcccgccg gacacggtcg tgctcaatgc cggcacgcac cagcccgtcg
    21361 ccgccaagga tttcaccgcg gacggcctga ggcaggtgat ggagctgaac gtcttcggtg
    21421 tcgccaacgg catcgaggcg gtgctgccgg gcatgctcga gcgcgatgcc ggccggatcg
    21481 ccgtggtcgc gtcggtggcc ggctatcagg gcctgccgac cagcgcctat tatggcgcca
    21541 gcaaggcggc gctcatcaac atgatcgaat cgctgcggtt cgacctccgt cgcaccggcg
    21601 tggtgctgca gctcgtcaat ccgggcttcg tcaggacgcc gctcaccgac cggaacgagt
    21661 ttcccatgcc cttcctgatc gaggccgacg aggcggcggc ggcgatcgcc cgcgggctcg
    21721 aggtcggtcg cttcgagatc gccttcccga ccgttttcgt ctggcttctc aagctcctcg
    21781 gcctcttgcc ctaccgcgcc tatttcgcgc tggtcggccg gagcaccggc aagtgagcgt
    21841 cgaggcggct gccggcgtct atgcccgcta tttcgacgag ctgacgacag cgaccacggg
    21901 cgatcttcgc cggctcgcgg cggcggacat gcgcttcaag gacccgttca acgacatccg
    21961 gggcgtggac cgggtggtcg gcctcttcga tcacatgttc cagacgacgg agcagccgcg
    22021 ctttgtcacc ggtgcgatcg ccgtgaccag ccgcaccgcc ttcctgcgct ggcgcttcac
    22081 ctgcaccgtg ccgagccggc tcctgtcatt ggcgctggcg atcgaggggg tgagcgaggt
    22141 ccgcttcgat gcggcagagc tggttgtcga gcatgtcgac tactgggatc cggcggcagc
    22201 cgtctacgag cgcctgccac tattgggcgg cgtcctgaga ggcctgcgcc gccgtctcgc
    22261 ccaccgcccc tgacagttcg ggcttcttga agaagatata gacctcgccg agggtgaagc
    22321 cccatttcga gacggtggtg cggttgatca tcaccgtctc gtcctggagg aacatccagt
    22381 cgtcgaagct gacgcgccag atccgatctc cgaccgcgag gtcgaagagg taggtccagc
    22441 gcatggcctt gccctcgcgc acccccgtcg cggtgccgac gatgtcgtcg gcctcgcctt
    22501 cgtagcggcc gtcgccgagc gggcggatgt gccaggtgcg ctggtcgcgc tcgccgtccg
    22561 cgtagcgaaa tgcctcgtcg aggacgagga cgtcgccttc catgcggccc tcgatgtcga
    22621 ccacgaactc ccggcgcacg gtgccgaagc ggtcctggaa caggccccag gcccgcgtct
    22681 cgccgagaaa gtaatcctcc ggcacgaagg ccggggcggt gccgtcgaaa tcgtcgatct
    22741 tcatggccgt gcaacccgca atgacacaga gcattacggc ggcgagaacg gtgcggatca
    22801 tcacgtcagg gcattccctc tgctcgcagc tcgccgaggc gggccgcgtc gatctcgaag
    22861 ttccagacga ggaagatcga ggcgaatttc atggcgaccg gcaggagcgc gtagagcccg
    22921 accagcgcca ggagcgcgat cggaccgttc gaagtcccct cggccgcgaa gccggcgacg
    22981 tcgagcgtgc cgaaggcgat gccgacggcg agcgccagcg agagcttggt cgccatgctc
    23041 cagagcgcga aatagagccc ggcgcggcga ttgcccgtca ccgcgctgtc ggcatcgacc
    23101 acgtccgcct gcatcgccgt gggcaaggcg agatcggcac ccaggccgag gccgctggcg
    23161 acgcagacgg tggcgaacca ccagacatcg ccggccccga gcagcggcac gagcgcgaag
    23221 acgccgcaga cccagagcat cgagagcgac caggcgcgat gcttgccgat ccggtagctc
    23281 agccagagcc aggccgggat ggcgacgagc ccgctggcga aatagacgag gagcaaggga
    23341 ccgctcgccc ccggcgcctc gatcacgtgc tggacataga ggaggaagag ggtcgcgggc
    23401 aggccgttgg cgacaccgtt cagaaggtag gcgaagacca gccggcgaaa gggccggttg
    23461 gcgaatgcca cgcctagccc ggcgacgaac ttcaggggcc gggtctcggc ataggcgctc
    23521 tcgccgatgc gccagaggag gacggcgaat gccaagggca ggaggagcgc catggaccag
    23581 aagatcgccg ccatggtcgt cgcgctgccg ggccctgcac cgatcagcgt cggcagggcg
    23641 gcggccagga tgatgccgag gatgacgaag ccctcgcgcg cgccgaagat gcgtgagcgc
    23701 tcgtcgtagt cggtcgagag ctccgccccc caggcgctgt aggcgagcag cagcatggtc
    23761 caggcgagaa aggcgacgct cgcccagagg aagagataga gcccacccgc accttcccgc
    23821 ggcaggaaca ggaagaagct cgcgaccagc agcaggggca gcgcaccgag catccagggc
    23881 cgccgccggc cgaagcgcga gcgcgtgcgg tcgccgagcg tgccgacgag cgggtcggtg
    23941 accacgtcga gcagccgggc ggccagcagc acggcaccga cggtggcgag gtcgaggccg
    24001 agctcggtga catagaaggt cggcaggtag acgtagaacg gcaagttgag ggcggcgagc
    24061 ggcagcgcca gccctccgaa cgccaggaga tccatgagcc cgagccgccg gccgggcggc
    24121 ggcgtttcag ctccggcgca gggcaagctg cacgacgtcg gtgctgccgg agcggaagcc
    24181 ggcctcgcag tagccgagat agtagcgcca gacacgacgg aagcgctcgt cgaagccgag
    24241 ctggcggatg ccgtcccagg ccgtctcgaa gcgctcgccc cagagggcca gcgtgcgggc
    24301 gtagtcgcgg ccgaaggcga ccttccgggt gatctcgagg ccgacccgcc ccgtgtgctc
    24361 ggcgagggcg gtggtcgacg gcagcatgcc acccgggaag atgtattgct ggatgaagtc
    24421 gctgcccttg cgatagctct cgaaatgctc gtcggcgatg gtgatgatct gcagcccggc
    24481 catgccgccc ggcaccagcg ccgagcgcac cttctcgaag taggtcggcc agtaggcttc
    24541 gcccaccgcc tcgaacatct cgatcgaggc gatccggtcg aacctgccct cgacgtcgcg
    24601 atagtcctgg aatcggatct cgaccttctc gcggagcccg gcctcggcga cccgggcact
    24661 ggccagctcg tgctgcgcct tcgagatcgt gatgctggtc accttcgccc cgaactcgcc
    24721 ggcggcggtc agggcgaagc cgccccagcc gctgccgatc tcgagcaaat gctggcccgg
    24781 ccgcagctcg atcatccggc agagatggcg gtacttggcc ttctgtgcgg cttcgagatc
    24841 agctttttca gcggtgtgcc gctcgtcgaa gagggcggcc gagtaggtca tggaggggtc
    24901 gagccaggcg gcgaagaact cgttgccgag gtcgtagtgg gcatggatgt tgcgctggct
    24961 gccgcgtctc gagttgcggc ggaaccagtg ctgcagccgt gcgaggaagc gcgtcaggac
    25021 cggcccgaaa tagccttggc cccaggcatc gaaattacgg tcgagcagct cgagcaggtc
    25081 ggccagatgc ggcgtgtccc actcgcccgc gatgtagccc tcggcgaagc cgacgctgcc
    25141 gtggcgcaga tagcggtcga ccaccttggc gtcgcggatg gccagggcgg cgcgcggtcc
    25201 cgcctcggcg ccccggacgg tctcggtgct gccgtccggc agggtcaggt cgagggtgcc
    25261 ccggcgcagc tggcggacga cgcgcagcac ggcctcgagc cgcacgcggt cgaccgcccg
    25321 gccggtcgtc gccctggtgt cggaggttcc ctggtcggtg cccatggtca tgatcgccac
    25381 tgctgctgcc ggcgaccgat cgttcgggct cgtcatccgt cgatcgggtc ggcggttcgt
    25441 gggtgaaagg gtgcgccctt cagccagagg cgcagcgcct cgacatgaat gccaccgatc
    25501 accttcaacg tcatggcaag attgcgcagg aggcagaggg cgaggttccg gtcgtcgagc
    25561 ggcagcgccc ggccggtctg gctcgcggtg aagaagagcc cgtcccggtc ggtctcgcgg
    25621 atcaccaggt ccagccgctc gcccggcggc ttaagggtga actcgtaggc tgccgccatg
    25681 tcgatgaagg gcgagacgta gaaggtcttc gccgccgcgt ggcgcagcca ggcgccggac
    25741 gcggcgaggg ccgggtcggt cggcaggaca taggtgtgct ggccgccgaa cgtgttcttg
    25801 acctcgtaga gcacggccat gagccggtcc tcggcatcat agccgaaata gacgctgagc
    25861 gggttgaaga cgaagcccca gagccggggg aagcagagga gctcgatccg gcggacgccg
    25921 tccaggccgg cggtggcgaa gtgcccgagc gcccagggtt cgagcggcga gccgtcgcgg
    25981 gcgccgtggt cgctggcgcg aaagccgagg atgccgggtc gctcgatgcc gagcaggcgc
    26041 aagcgcgagc caaccgcgtc gagctcgccc aggtcgagca gcagcgagaa gaccctgtag
    26101 cggaagcgat gcttgacggg gcgcagtcgc tgatggacca ccgttccctc gtagagccgc
    26161 gagcgcagcg cagggccggt catgccgatt gcgccgccgc cagctccggc agaccgtggc
    26221 ccgagcctgc catcggccgg gattcccgct tccagggtgc ggtgacgccg agccgggcgg
    26281 ccgcctcgag cccggagcgc agcccgtcct cgtggaagcc gtagccgagc caggcgccgg
    26341 catagtggat gccgccgcgc ccctggatcc ggccgatcgc gccctgcgcc gccacggcgt
    26401 cggcatcgaa gaccgggtgg tcgtaggaga actcgcggtg caccagcgcc ggatcgggct
    26461 cggcgagcgg gttcaacgag acgaagagcg gcaggctctc gtcgatgttc tgcagtcggt
    26521 tcatccagta ggtgacggcc acgcgactgc cgggctcggc gaggccgggg gcggtgtagt
    26581 tccaggccga ccaggcccgg cggcgcttcg gcatgagcgc cgggtcgctg tgcaggacgg
    26641 cgcggttcgg ctgatagcgg aaggcgccga ggatgcgccg ctcctcgtcg tccgcgtcga
    26701 gcagcaccgc tgccgtctgc ggcgcatggg ccgccatgac cacggcatcg aagcgaaagc
    26761 tgccgccctg gccgtcgacc acctcgaccc cggcgttcgt ccggcgcacc gccacgaccg
    26821 gcgccgcctt gcggacacga ccctcgagcc gggccgcgag tgcgtcgaca taggatttgg
    26881 aacgaccgct cagcgtgcgc catttcggct ggccggttac cgtcaggagt ccgtgattgg
    26941 cgaagaagcg caggaagctg gtcgccggga aatcggccat ggtcgccgtt gggcaggacc
    27001 agatggcggc cgccatgggc aagagatagc cgccggtgaa ggcttcgccg aagcggtggg
    27061 tccgaaggaa gtcgccgagg ctccaggcgt gcttcgcccg ttcctcgaga tgctcccggc
    27121 cgagccgatt gaagcgcagg atgtcgccga gcatgcccca gaagacgggc cgcacgagat
    27181 tgcgtttctg ggcgaagagc gctgccggcg aggcgccgct atattcgagg gtgccgccgg
    27241 cgatgctcgc cgagaacgac atctcgctgg cctcggttgc cacgccgaga tggcgcagca
    27301 gcgaggtcaa ctcgggatag ttgggctcgt tgaagacgat gaagcccgtg tccacgtcga
    27361 ccgaccgctc gcccgccgat accgtcaccg tgttgctgtg cccgccgagt cgctgctcct
    27421 tctcgaagag ggtgacccga tggcggtgtg ccagaagcca ggcagcggaa agcccggcga
    27481 tgccagagcc gacgacgccg atatgcaagg aagctctccg ttggctggtt gcggctctga
    27541 tcccttgtgg gccagttgca cgcgatcgtg tccctcgcca tcgagcggcg gcagcggcgg
    27601 ggcccgccga ccccggggga gggtaggtcg gcgggctttc gccactggct tcgcagccgt
    27661 ctggctaggg atggatacgc agcgcggtca ggactggatc acggcgatgg tcgatcgtcg
    27721 atccgacctg gagagcgagc atgcgagatt gccgaccccg ggctcagccc tggccgccca
    27781 gcgcgcgcgc ctggccgatc cagtcctcgc gctcgatgcg tcccttgaag tcgagcatct
    27841 cgtcgatccg ctcgatgtcc gcaggggtca aggccgcgat ctggctgaag gtggtgatgc
    27901 cgagatcgtt cagcttctgc cgcagcaccg ggccgatccc gcggatcttc tcgagatcgt
    27961 ccggcgcatc ggccggcggg gtgaagagct ggaccgggtt cggcgccggc gccgccttgg
    28021 cctcgccgcc gtcgagcgcc gcccgctcgg ccatcaggcg cttcctggcg gaggagccgg
    28081 cggggagctg cgccagcttc gcatcgaccc gccggcgtgc ctgggccagc cgctcgacct
    28141 cggcgggcac ggccggagcc ggggccggcg ctttctccgt taccaggggg gccggtgctg
    28201 cggcggccgg cggcgtcgct tccagtgcca ccttctcctg ttggcagagc cgcagccgct
    28261 ccttggtctc gtcgtgcgcc gcgtcggccc gccgcacctc ctgctcgaca tgggcgagct
    28321 tcttcttcca gccttcctcg agcgttgccc ggctggacgc gtcgcgccgc tcgaagaacc
    28381 accaggcggc gatgacgccg acgatgatgc cgacgatgaa catcaagaat gagcccatgc
    28441 gcaattactc cctgcatcca ccggcggcga ccggtcggcc gcccataaat cacacccgat
    28501 gacgttttcc cgttgcggcg atactggccg gcaaggcgcc gcagcacaag tcagcccctg
    28561 gcatcctcga cgatcatccg ggcggccttt tcggcgatca tcaccaccgg cgagttggtg
    28621 ttgcccgagg ttatggtcgg catgatcgag gcgtcgacca ccctgagccc gccgattccg
    28681 tgcacccgca gccgtgcatc gaccaccgcc gtgcggtcct ccgccggccc catcctgcag
    28741 gtgccgacgg ggtggaagat ggtggtgccg agcgcgcccg ccgcctgcac cagctccgcc
    28801 tcggtggcca cggccggacc cggcaggtgc tcctccggcg catagggagc gagagccggg
    28861 gccgccatga tggtccgggt caggcggatc gcgtccgcgg cgaccttgcg gtcctcctcg
    28921 gtcgccagat agctgggggc gatcgccggc ggtgctgccg ggtccctgga ccggagccgg
    28981 actgtgccac ggctggtcgg ccggaggttg cagaccgagg cggtgatcgc cgggaaggga
    29041 tgcaggggct cgccgaagcg gccgaggctc aatggctgga cgtgatactc gacattgggc
    29101 gtcgcgaagc tcggatcggt cctggcgaag gcgccgaggg tcgagggcgc catgctcagg
    29161 ggtccccgcc ggtggagcgc gtattccagg gccatccgcg ccttgccggc gaggctcgcc
    29221 gcctgctcgt tcagggtcac cgcgttctcg accttgaaga cggtgcggat ctgcagatgg
    29281 tcctggagat tgccgccaac cccgtcgagc gcgtgccgga cctcgatctc ctgttcccgg
    29341 aggtgcccgg ccgggccgat gccggcgagc agcatgagct gcggcgagcc gatcgagccg
    29401 gcggcgagga tcacctcccg cccggcagcc accgatttct caaggccgtt ctcgaaataa
    29461 tcgacgccgg tcacccgccg gccgtcgagc ctgaggccgg atacccgggc accggtcacc
    29521 acgatcaggt tggggcgacg gcgcacgggc ttcaggaagg ccctggcggc ggaccagcgc
    29581 acgccccggc gctggttgac gtggaaatag ccgcagccct cgttgttgcc gcggttgaag
    29641 tcgtcggtct tcggaatgcc gacctcggct gccgccgcgc ggaacgcgtc gaggatcggc
    29701 caggcgaccc gcggctcctc gacccgccac tcgccgccga cgccgtgcag ctcgtccgcc
    29761 ccgccgcaga aatcctcgga gagcttgaac cagggcagca cgtcgtccca gctccagccg
    29821 ggattgccga gctgccgcca gtggtcgtag tcggccgcct ggccgcgcat gtagatcatg
    29881 ccgttgatcg aggagcagcc gcccagcacc cggccgcgcg ggtagttgag cgcccgcccg
    29941 ccgaggccag gctcggcctc ggtcttgaag caccagtcgg tgcggggatt gttctgggtg
    30001 tagaggtagc cgatcggcac gtggatccag aaccagttgt cacggccccc ggcctcgagc
    30061 agcaggacac gggtgccggg gtcggccgag aggcggttgg cgagcacgca gccggcggtg
    30121 ccggcgccga tgacgacgta gtcgaagccg ttcacgcaac tccctcctga cggacgcggg
    30181 gaaggttacc ggccgacagc cgacgcggca accgcttgcc ccgccagcgc ttcgtggcca
    30241 ccttggagct tcattggaat gtatccagaa cggagcgctg cttgcgtgac atggcgggat
    30301 cggccggcgg gaaggccctg agttgagccg cgggcgcggc gtgccgccgc cattgtccgc
    30361 gaccctcggt gctgtgggtc ggccggaacg gagctgggcc gatctcgccc gcttcgcgct
    30421 gccctatgtc tggccggagg gcgagccccg gctgcgcctg cgcatcgtcc tcgccttcgc
    30481 ctgcctcgtt gccgccaaga tcctcacggt ctacgtcccc ttcttcctca aatggagcgt
    30541 cgacgcgctg agcgtcgcca atctcaccgc cgtgccgctg gcattgctga tcggctatgg
    30601 cgccgctcgc ctcctcgcct cggccttcca gcagctccgt gacgcgctct tcgcccgggt
    30661 gggggcccgg gccggacggc gcctggccct tcaggtctac acccgcctct tcgacctctc
    30721 gctccgctat cacctcgagc ggcggaccgg cgagctcgcc cgggccatcg accgcggcgt
    30781 caaggcgctc tcgttcctgc tgcgcaccgg cctcttcagc ctcgggccga cgctgctcga
    30841 gttcgtcatg gtcatcggcg tgctgctctg gaactacccg ccgagcttcg ccctcgtcac
    30901 cttcgtcacc atcgtcggct atgccgcctt caccatcctc accaccaact ggcggacgat
    30961 gatccgccgc gagctcaacc gcgccgacaa cgagttcaac gcctcggcgg tcgacgggct
    31021 gatcaattac gaggtggtca aggcgttcgg caacgagggc ttcgagcggc ggcggctcga
    31081 cggccagctc gccgcctacg agaccgcggc ggtgcgctcc gaggtctcgc tcgcctggct
    31141 gaacggcggc caggccgcga tcctcgccgt cggcgtcacc atcctcatgg tgatggccgg
    31201 ccggggcgtg gtcgggggct ccttgaccat cggtgacgtg gtgctgctca acaccttcat
    31261 ctggcagctc taccagccgc tcaacttcct cggcgtgatt tatcgcgaga tccgccagtc
    31321 ggtgaccgac ctcgagcaga tcgaggccct gctcgagctg gagccggagg tggccgacgc
    31381 agccgacgcc gtgccgctca tcgtcaccga cggcaccgtc cgcttcgcag aggtgcgctt
    31441 cgcctacgac ccgaggcggc cgttggtcga cggcctcgac ttcaccctcg agggcggccg
    31501 caagctcggc atcgtcgggc cgacgggggc cggcaagtcg accatcgtgc ggctcctctt
    31561 tcgcttctac gaccccgacg gcggccgggt gaccatcgac ggccaggacg tcagccaggt
    31621 gacccaggcg tcgctgcgcg gccaaatcgg cctcgtgccg caggacaccg tcctcttcaa
    31681 cgacaccatc gccgccaata tcggctatgg ccgccccggt gcgtccatgg aagccatcga
    31741 ggacgccgcc cgcctcgccc agatccacga cttcatcgag agcctgccgg acggctaccg
    31801 gacgacggtc ggcgagcgcg gcctgaagct ctcgggcggc gagaagcagc gcgtcgccat
    31861 tgcccgcgtg atgctgaaga acccgccgat cctgatcctc gacgaggcga cctcggccct
    31921 cgacagcacc accgagcgcg ccctgcagga ggccctcgcc cgcgccgcca agggccgcac
    31981 cacgctggtc atcgcgcatc gcctctccac catcaccgac gccgaccaga tcatcgtcat
    32041 ggacgagggc cgcgtcgtcg agcgcggcac
//
LOCUS       DAOTFI010000014        31823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1594_length_31823_cov_6.730956, whole genome shotgun sequence.
ACCESSION   DAOTFI010000014 DAOTFI010000000
VERSION     DAOTFI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 31823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31823
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_31823_cov_6.730956"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /gene="nth"
                     /locus_tag="P5558_02370"
     CDS             complement(<1..519)
                     /gene="nth"
                     /locus_tag="P5558_02370"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRY23187.1"
                     /translation="MQRQPGNAKSARGGRPPTGRPKPRPAARSRLGRAAILELFATLA
                     RDRPAPATELHYADPFTLLVAVVLSAQATDVGVNKATARLFARADTPAAMLELGEEAV
                     RESIRTIGLYRNKARNVIELSRLLLERHGGQVPNDRAALEALPGVGRKTANVVLNEAF
                     GEATIAVDTHIFR"
     gene            547..1041
                     /locus_tag="P5558_02375"
     CDS             547..1041
                     /locus_tag="P5558_02375"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HRY23188.1"
                     /translation="MTDDASAGTGPAAFEAVLYPNQPPPTSSLVLLVVAVASVAVGMS
                     IGFYLAGAWPVSGFVGLELGLLIAALLWTRRGARYREHVRLDDTGLHVRAIAGNRVLR
                     AWRFEPYWVRVGLEPVRHGERVLQLSAHGRTLTLGRFLTSAERCDFAAALNRALGRYR
                     ENSG"
     gene            1388..1852
                     /locus_tag="P5558_02380"
     CDS             1388..1852
                     /locus_tag="P5558_02380"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRY23189.1"
                     /translation="MNEPQQGVPEAGAHRLPVSKLRGVPQMARVRLKSLRITTCGKLL
                     AAAATPAGRERLAAATGIDPDLLLQLVRRADLARINGIGTVFGLMLEALGVDNVARLA
                     REDAASLHQALRSFNKEERLARRSPTPEEVAEWIEAARALPPCVAEEPVEGD"
     gene            2022..2522
                     /locus_tag="P5558_02385"
     CDS             2022..2522
                     /locus_tag="P5558_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRY23190.1"
                     /translation="MPRLPHLMGYSRAQIRLHWLVAVLITLQYLLHEPIVAAWRAFRK
                     GETLAFQPLVALHVAGGLAILALVLWRLWLRYRRGAPPPPEAEPAPLRLAARAAHVTL
                     YALMVAMPLSGAAAWFGGIGAAAALHGWLRIVLLVLIGLHVLAVLAHQLVFRTSLMQR
                     MWRAEI"
     gene            complement(2479..3075)
                     /locus_tag="P5558_02390"
     CDS             complement(2479..3075)
                     /locus_tag="P5558_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY23191.1"
                     /translation="MNAITIEAIGSDRFAACLDDLSGILHAAVNGGASVNFILPYTPD
                     DARAFWLEKVRPALPNGGRILLVARCRERIVGTVQLILDLPPNQAHRAEVTKLLVHPD
                     SRRCGIGERLMIELEAQARAHGRSLVTLDTTTGSAAERLYRRLGYRVTGCVPAFSLAP
                     DGSGYDATTYMYKVLRAAVEGGPAVRSPPATFAASNSS"
     gene            complement(3077..3637)
                     /locus_tag="P5558_02395"
     CDS             complement(3077..3637)
                     /locus_tag="P5558_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY23192.1"
                     /translation="MDDGLTSRLAERLRQLRQERGWTLDHLAALAGVSRATLSRLENA
                     EVSPTTDVLGRLCVAHGLSLTRLLAPVEADFAPVVRREAQPLWRDDEAGFLRRAVSPP
                     AGPLAAEIIEGTLRPGADLAYAAPPVPGQEHHLVLLEGRLVVTLDGRCHALEPGDCLR
                     YRLAGPSAFAADAEAGARYVLVLVGA"
     gene            complement(3727..5115)
                     /locus_tag="P5558_02400"
     CDS             complement(3727..5115)
                     /locus_tag="P5558_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY23193.1"
                     /translation="MSTTGLVNRSGIAIAADAIDAFAAGLAGRVIRPADADYDSARRI
                     WNATIDRHPGLVVRPLGTADVIAAVTFARQHDLLVAVRGGGHNVAGRGLCDGGLVVDL
                     SLMRGVLVDAASRTVRVQGGATLGDVDRETHLHGLAVPTGVVSRTGIAGLTLGGGVGW
                     LVRKHGFTCDNVLAFELVDASGEHRTVSATEHPDLFWALRGGGGNFGIVTSFTFEAHP
                     VKTVLGGLIVHARDQAGAVLRYYRDFMKTAPLELTAYAALLTTPDGHPAVAVIPCWCG
                     DMAEGERAVAPLRQFGSPLMDAVQPMPFPVMQSMLDDAFQDGTRNYWKSTFVKALDDD
                     AIDTLVGHANRMTSPLSAVVVEFYAGAASAVGPAETAFAQRQAEYDIGFMAQWTDPAE
                     DATHTAWARAAWDAMQPFSSGGFLLNFLSEESPDVIRAAFGSNYDRLAELKRKYDPTN
                     FFSLNQNIKTAA"
     gene            5710..6150
                     /gene="rnk"
                     /locus_tag="P5558_02405"
     CDS             5710..6150
                     /gene="rnk"
                     /locus_tag="P5558_02405"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HRY23194.1"
                     /translation="MDAEAKKHAKPVITLTQSDHDSLTGMARTTRGEVAEQLFEELER
                     ARVVPDHKLRPGIVRMGSTLRFSTDSGESREVTLVYPGEADIAAGKVSVLTPIGAALI
                     GLAAGQSIDWVTRDGRRRRLTVENVVDARPAPAEAPAAAVQPTP"
     gene            6192..6575
                     /locus_tag="P5558_02410"
     CDS             6192..6575
                     /locus_tag="P5558_02410"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRY23195.1"
                     /translation="MVTLPTIRRSAWLLLGLVCVAVAVVGIFLPLVPTTPLLLLAAFC
                     FARSSDRLLRWLHEHPRFGPTLLHWAEHRAIAQRAKVMAVLAMAAAIGLAIGLGAPFW
                     VVLVQVAVLLPVALFIVTRPEGPRA"
     gene            6852..7241
                     /locus_tag="P5558_02415"
     CDS             6852..7241
                     /locus_tag="P5558_02415"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY23196.1"
                     /translation="MNIRHTPPTVAAPFGPYAHGVEVPGGSRLLYISGEVGVLPDGTL
                     PDGIEAQADACWANIEAILADAGMGVGDLVKITTFLVRPEDLAAAGAVRAKHLGEVRP
                     GSTTIVVEALVRPEWLIEIEAVAAAKG"
     gene            7246..7860
                     /locus_tag="P5558_02420"
     CDS             7246..7860
                     /locus_tag="P5558_02420"
                     /inference="COORDINATES: protein motif:HMM:NF025850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY23197.1"
                     /translation="MAEFKVTYFDMAGSRGEDVRLALVIAGRTFEDIRVDREAFLKLK
                     PELPFGSLPVLEVEGHGVFSQTNAILRLIGRLCDLYPQEPFEAARHDALMDAVEDLRH
                     RIGPTARIQDAAAKKAARQQLATDLIPRWGEGVERLIGQGPFVGGERPGVADIKLYMA
                     HRWLGSGVLDDIPKDVLDHCPKLEAVANGIATHPAVRDWYAKAG"
     gene            7942..9705
                     /locus_tag="P5558_02425"
     CDS             7942..9705
                     /locus_tag="P5558_02425"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY23198.1"
                     /translation="MTAERTIRRLAAILVADVVGYSRLMAEDETAALAALNRHREVEF
                     DPIVARHNGRIVKLMGDGTLVEFRSVVDAVTCAVAIQTAAVAPGSSPAMMLRIGVHLG
                     DIIIQGDDIYGDGVNIAARLEPRAAAGGVCVSSVVNESLRDRVDTVFVDGGDVAVKNL
                     ARPVRVWHWHPGDGSAPAPAVPPPARASEGRSIAVLAFANMSGDAEQEYFSDGIAEDI
                     ITDLSKVAGLLVIARNSSFAYKGRSVDLRDVGRELGVGHVLEGSVRRAGGRVRITAQL
                     IDTATGGHLWADRYDRELTDVFAVQDEVTLKIVDALRVRLSPAERADIVAAGTTSMEA
                     HDCFLRMRNLIFSPGLTPALWQRGIAEGLRAIELDPTYVQARALTSICHWLDFHNGWS
                     GNAPEVVAARARALADRAIELDPADPFANHAIAVAARWQGDYDLAAEAIDRTLAKNPD
                     YALGLFTRSEIALAAGRHTDAIADLEHATRVDPAWTHQYLQNMGMAHLLLGHVETAAL
                     ILRERLLLARDTDIGRAWLASALGHLGEIEEARRLWAELLEINPSFSIEQRLARQRFA
                     RRADIDQVMAGLARAGLPVGP"
     gene            9820..10314
                     /locus_tag="P5558_02430"
     CDS             9820..10314
                     /locus_tag="P5558_02430"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HRY23199.1"
                     /translation="MTKIALPLVLGLGLTIGLAQVQDAHADSTGCGLGTTVWKGESGV
                     GPQVLAVTTNGTSGNQTFGITSGTLGCEQNATIASVEVRNLTVANLDNLATDMARGEG
                     ETLTSLAVAMQVEPQDQPVLFATFQQNFTRIFPSEDTSAEDVLVAMREVMTENETLVR
                     YVEV"
     gene            10493..12349
                     /locus_tag="P5558_02435"
     CDS             10493..12349
                     /locus_tag="P5558_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRY23200.1"
                     /translation="MPAEPAYLDELVASAEAQGLATAPGWETLGHYRPNLLGRGRKSL
                     IDSESFFLAKDGKSDPASELEATLAAFFAPAGEPALQHPQCRFVARYRWLKDRLGFDP
                     DRLPEQDCAEFDAWLATIDATSVTLIFPAAYLNNPASMFGHTLLRFDRPDQTEETRLL
                     SHAVNYGAWTGTDNGVLFAVLGLTGGYEGTYSVEPYYELVKRYSDLENRDIWEYQLDF
                     SPTEVERMLAHLWELRDQHSDYYFFDENCSYQLLFLLDAARPKLDLVGDFDLHVIPAD
                     SVRSVVRREGLLRHTVFRPSGETRLQQGLVAMPPADRKLVRGLASGAISPGEAAASNM
                     SPPRLAAVLELAEASVTHELRAGDLERDVAAPRAWSLLAARSEIDTPADRPPVPVPAT
                     RPDQGHGSARLAAGIGVRDGDVFQALRLRPAYHDGLDPAGGFARGAGIEALDLELRHY
                     QGDNSVVLDRFTAVRISSMAPRDELIRPVSWRLDFGLERMRVERGDTKGALVGVAEGG
                     AGLGYGLGQQGVWSAMADIGLSGGEDCDRTCSVNLGPALSLLWPITDRATFTASGSYR
                     VRFGERVRDGYELRLGQSYGLAADLAIKVELALENQGGGMQPELLSTLNWYF"
     gene            12349..13203
                     /locus_tag="P5558_02440"
     CDS             12349..13203
                     /locus_tag="P5558_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY23201.1"
                     /translation="MTRVRSLAALALLLALGACTGLFFQPMKSHVFDPASIGFTYRDV
                     DFEAVDGTALHGWYFPAEGEPSGSILFLHGNAENISTHFANVAWLAKSGFAVLVFDYR
                     GYGRSAGSPDLGGLHLDVAAALDALLRQPETDPDRIAVIGQSLGAALALTAMAASPMK
                     DRLRAVIIEGAFVGYPEIAREKLAAFWLTWPLQQPLSWTIDGRYSPLEAAAEIAPVPL
                     LVILDEADTIIPPHHGEALFAAAGEPKTLWRLPGSGHIQALATVGNRHRLVEYLQAVM
                     AAPPMPTP"
     gene            complement(13214..14248)
                     /locus_tag="P5558_02445"
     CDS             complement(13214..14248)
                     /locus_tag="P5558_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY23202.1"
                     /translation="MLFRQLFDKTSSTYTYLMASRAGGEALLIDPVLEQTGRYLKLLE
                     ELDLKLVKVIDTHIHADHVSAMGRLRDATRCVTVMGEQSPADVVSMRVSDNERLTIEG
                     IALTALHTPGHTSESYSFLMDDRVFTGDTLLIRGTGRTDFQNGDPYDQYRSLFDRLLK
                     LPDQTLVYPAHDYKGDTVSTIAEEKAHNPRLKVGSADEYAEIMNNLNLPNPKMMDVAV
                     PENLRVGLQLEAQHRVPVLEVEALLATWPEVDGQLIDIREESERRRDGAIPGSMHLPY
                     GQFASSCDASGPLRRLGRDARLVLYCAVGERSTLAVEIAAEHGVRNTAHIPGGFRAWR
                     SAGGPVETIG"
     gene            14411..15133
                     /locus_tag="P5558_02450"
     CDS             14411..15133
                     /locus_tag="P5558_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HRY23203.1"
                     /translation="MPITVLAETRLIEIAAPFVFLVCWIGYWAFADQLQLGNRSLMRR
                     MHEYRRAWLMQMLVRDNRIVDIQVVQVLVQNVSFFASSAILIVGGFVAVLGASEQARA
                     IVAEIPFAEDPSSLIWDLKILLLIVIFVYAFFKFTWSLRLFNYVAILIGAAPAGRSPD
                     TIAMAEQIAVVADRASDHFNRAMRAFYFGLAALAWFIQSWLFILVSVLVVVIIYRREY
                     RSAVLTVLGPVGRSIPGAPEEG"
     gene            complement(15130..16128)
                     /locus_tag="P5558_02455"
     CDS             complement(15130..16128)
                     /locus_tag="P5558_02455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRY23204.1"
                     /translation="MIPFSVLDLAPIAQGSNASDALRNSLDLAQHAERWGYHRFWLAE
                     HHNMPGIASAATAVLIGHVAAGTTRIRVGAGGIMLPNHAPLVIAEQFGTLATLHPGRI
                     DLGLGRAPGTDMATARALRRHLDAGDDFPRDVMELMAYLGPEQPGQIVKANPGTGTEV
                     PIWILGSSLYGAQLAAHLGLPYAFASHFAPAALDQAAAVYRQTFRPSARLQQPRFMLA
                     LNVFAAASDAEGHYLRSSMLQAFVNLRTGRAGPLPRPVEDVERHLDPVALASAEQALA
                     ITAVGAPDTVRRGLQDLIARYRPDELILTGQIHDHAARLRSFEIAADILGALEYPA"
     gene            complement(16125..17225)
                     /locus_tag="P5558_02460"
     CDS             complement(16125..17225)
                     /locus_tag="P5558_02460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRY23205.1"
                     /translation="MRLLIVTDAWYPQLNGVVRSIERMMELAAGCGAEIALLTPQHFR
                     SVPLPSYPEIRLALARPGRLARLIARLAPTHVHIATEGPLGILTRRLCVRHGLPFTTC
                     VHTRLPDYVRARLPIPTDWTWRLMRWFHNGGNGVMVATDSLAQELDRHGFANIIRWSR
                     GVDTAQFDPARRTGLGCEGPVFLYVGRLAIEKSVDDFLRLDLPGTKLVVGDGPARRQL
                     EAAFPDARFLGKLTGLELARAYASADVFVFPSRTDTFGMVLLEALASGLPIAAYPTAG
                     PLTIVGSSGTGVLDHDLRRAALGALDIPRERCRAHALGFSWEASAGQFFANIRAGNRG
                     MTIDASVLDRPRLVERARRRLGWRPATVSPGT"
     gene            complement(17368..20046)
                     /locus_tag="P5558_02465"
     CDS             complement(17368..20046)
                     /locus_tag="P5558_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY23206.1"
                     /translation="MRLDELVVEDFKKLKGRHVVRPAALGITVVSGDNEEGKSTLLDA
                     LKAAFFMKHNTTGADRDAIQPFAGGETPAVTVGFTLDGSAYRLAKRFRRGGVQLETPE
                     GLLEGDAAELRLASLLQFEWPGRGAARDTHTGVAGLFWVDQGTTFAKEQEPSPTALRG
                     LQPALADQVAALGRGERAPRLMTEVRRRRDQHWTGKRDQPRGELATLEREVAELRVEL
                     SGLEAAEAEVEALLDRLAATLEKRRAWLAADHLGRARRAVDGSRSALAGVRALEEEKR
                     VRSAERQAAASELRRLQDMADLRRQRLAAIDERAAEQAKLARATAENAPLLDRARRQR
                     AATDAAAEASRRALAAGERERETVRRRAERLRLARELAALERLAADVRATAAEAAEQQ
                     ARADAEAIDEIALEQLETMEVELKETRAALRAVATRLVLRPDRADAEVLLGLVPVDPA
                     APIELTAPATLILPGFGRIEVTPGGKGLAELRRALADGEAAFAGRLAELVARDLAEAR
                     RRLLARDRHAHEAEAARQKLATLLRASGMKGQAALDAACVTARARLAGFDDVDGGDDA
                     DDATLEAEREAVERTLAELREREAALAEERRRATEEEARLGREADVLAGAEARAGLEL
                     AKLEDQLAIERDELADVALAEALAAARTVDDRAAEAVLRVERELARAAPEAARLEAEQ
                     AARRLAQLEEEGRGLEREVVRLEAELKGAGGRGIGDRKGDVQGRLELAERRHARLFAE
                     ARAWRLLAETLEAVDRARQDALVEPLEARLAPYMRQLMGEAVAAFAPESLRLETLRRS
                     AASEPFSSLSVGTREQLAILVRLAIGDLLVETRGQSPPLILDDALVYADAVRLARMKT
                     ILQQAAARQQILVLTCRKEDYLGLDARYLRLEDCRA"
     gene            complement(20046..21170)
                     /locus_tag="P5558_02470"
     CDS             complement(20046..21170)
                     /locus_tag="P5558_02470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRY23207.1"
                     /translation="MTIRLLHTADWQLGKSFARFAGGDLAALLREARLAVIERLAARA
                     TEADVAAVLVAGDVLDHEHAEPGLLRRMVHRMAGFPGPWLLLPGNHDPARPGGLWDRF
                     AASGDCPENVRLLTRPEPMTLLDGRLVVLPAPLLQKHVSGDTTAWFDTAVLPAGAVKV
                     GLAHGGVPALLPGQSESPNPIALDRAERAGLDYLALGDWHSVLQVGPRTWFSGTPEPD
                     DFTRSGQGQALLVTLPGPGQPVTVRALETGRHAWQARKVDLGHGPAVGAIGDQLDALV
                     AGLAAPGETVLRLTLEGALDLAGRAALEAALGRLEARLRHLEVQDEGLLTAASEADLA
                     AMASEPLLAEVVAALADSEDPAAPVALRLLHQTWQRLEPG"
     gene            complement(21204..21569)
                     /locus_tag="P5558_02475"
     CDS             complement(21204..21569)
                     /locus_tag="P5558_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRY23208.1"
                     /translation="MSEAEAWPVEIRVHREERRLGIDFDDGRSFSYPAELLRVESPSA
                     EVQGHTPSQKVTVGGKRRVGIVRLEPVGNYAVRIVFDDGHSTGIYSWSWLYRLGAEQA
                     TIWAAYLAEIERQGLSRDG"
     gene            complement(21562..21822)
                     /locus_tag="P5558_02480"
     CDS             complement(21562..21822)
                     /locus_tag="P5558_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRY23209.1"
                     /translation="MTDTPETPTSGAAIDPALLDLLICPLTKGPLRYDRARNELVSEQ
                     AGLAYPVRDGIPIMLVDDARQLDDSDRIPDAVRADKDDHGNE"
     gene            complement(21850..22506)
                     /locus_tag="P5558_02485"
     CDS             complement(21850..22506)
                     /locus_tag="P5558_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRY23210.1"
                     /translation="MPGLESLPGRFPIFPLPGAVLLPGGNLPLNIFEPRYLQMVRDAM
                     QTDKVIGMIQPRGQAGGGEAAEGQPPIYGTGCAGRISSFRETEDGRFLITLTGIARFD
                     VAEELAVTTPYRQVVAAFDRWHDDLQPEPPPDALRGDLLTALKGYFERQQIEADWETI
                     KDAPLAGLVTSLVMICPFEPNEKQALLEAANLNAQAELLIALMCMAGTTGAAEPSVLH
                     "
     gene            complement(22526..23449)
                     /gene="trxA"
                     /locus_tag="P5558_02490"
     CDS             complement(22526..23449)
                     /gene="trxA"
                     /locus_tag="P5558_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRY23211.1"
                     /translation="MQMIGGTQAPAGQQGGGADLIKDGNLESFARDVIQESMRQPVLV
                     DFWATWCGPCKQLTPVLEKVVKQAGGKVKLVKIDIDQNPELAQQLRVQSVPTVYAFAQ
                     GQPVTGFAGAQPESQIKALVEKLTGGPLGPASVDVQLEEAKAALEAGELDAAGELFGG
                     VLEQEPENPEALGGLARCLLAAGDLAEAEQLLGDVPAAAAKHAAVTGAKSAVALAKEA
                     GTLGDPAELARRIEGNADDHDARYDLATALFLRGQVEQAMEHLRQIVKRDREWRDDGA
                     RKQLVRFFEALGPTHPLTIKGRRLLSSVLFS"
     gene            23672..23746
                     /locus_tag="P5558_02495"
     tRNA            23672..23746
                     /locus_tag="P5558_02495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23704..23706,aa:Gly,seq:gcc)
     gene            24195..25307
                     /locus_tag="P5558_02500"
     CDS             24195..25307
                     /locus_tag="P5558_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY23212.1"
                     /translation="MRLGWLAAGVAMVGLMGSDAVAADKYKIFLSMSYIGNDWQAEAA
                     NMVKAMADHASMRDKVDLQIQVAGPNAQRQIQQINAMVQQGADAIIVYPISPTALNQV
                     VKNACDKGVLVFAYDSVITEPCAYNTTIDQDEAGRKPAEWLVDQLGGEGKIVVITGVP
                     GTSVDTARTAAAKDVFAKHPGIEIVAEGNGMWSQAVARTELSKIVATHPWSEIDGLWM
                     QAGCFTAASMQDEAGIADADKKPCAGEGSNGHRIQMLPIGTEGIEGANGTYRPMGYPS
                     ISYASPPYSGALALKNAVAKLEGKEIPQLTSLPLPLYTNDEIKLCTEGTWAEMQAGCN
                     VFQPALVSNPGWFGSIYSAETPEIGLQAALVGQPEN"
     gene            25364..26905
                     /locus_tag="P5558_02505"
     CDS             25364..26905
                     /locus_tag="P5558_02505"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY23213.1"
                     /translation="MTEAAPALRVEKVAKAYGATVALDGASFAVRAGEVHALLGENGA
                     GKSTMVKLLSGLVQPDSGSISLAGQEVRLRRPAEAHRHGVQTAFQEMTLVRDLTVAQN
                     MLLPNAPTLLGQLRGRLGERRVAEHLARLGLESIDPRAEIRDLELPQRQKIEIARAVF
                     RRPRLLLLDEPTSTLSGRDIDWLGELIAGVGQDGVTVVFISHRLSEVRQLCDRLTVLR
                     NGRDIGTAEVDAIGDDEVIRMIIGRSLAATFPPRPAPRPWPADAVPVLAASGLATDGK
                     LADAGFALRPGEILGVAGLQGMGQRELFLACFGMLALRRGEIRVDGRKVTLASPKDAV
                     RARIGISLVPEDRKTEGLFLRLDGRRNISLPVIDRFTRWGLIDEARETRAVAGVLAEV
                     EVQQRAIYTSAGAFSGGNQQKIAIGKWLLAGSRILLMFDPTRGVDVGTKHQLYLMMRR
                     FADAGGSILFHSTEIAELVNLCDRVMVLYAGRVALELEGEPIEEQAIMRAALGELARP
                     AGIAA"
     gene            26902..28956
                     /locus_tag="P5558_02510"
     CDS             26902..28956
                     /locus_tag="P5558_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23214.1"
                     /translation="MSAAALPLAGRRPRRGMRLGRHRGLLVALVVFALLFAVADAITP
                     GSFSYFELSFMSSGGATLALAGMGQTVVVLTGGFDLSAGAVVSLVNVVLATWMGPEPP
                     SQILFGLVAIGIGATVGAVNGFFVAVVRLQPIVVTLSTMFIVQGITLLIMDKPGGLIA
                     PEFSAFLAGDAIPDRLPAPVVVIAAAVAFWLLLKNTRFGVALYAIGSDPEAARAAGLP
                     TRAIQLGAYVVAGMFYGAAGAFISAQTGSADPLIGGPMLLQIFTAVVLGGTLLGGGRG
                     GCLGTVVGAYTLVLVVNILLVLNVSAYYSSVAEGAILIMAVLAASLNRRSAIADYLRL
                     GWLELRARHDGTAPSAHAGEARPFALQRPEVALAPVSWLERHRELLRYVLPAYAGFVL
                     VLVATHLVYGNALTNPAYFNSLIVLASFLVVLALGQGAVILTGGLDLSLPWMIGLCGI
                     LVAGLVRGSDEAALWAIPLGLALGAGLGALNGAGIVLLGLPPIVMTLAMNGILQGAAL
                     VYSNGTPDGFASPGMRWFMTGRLLGVTPVVWFVAAFVGAALVLLGRTAFGRRVYAVGN
                     GALVARLSGVGVGGTLIGVYALSGLCAALVGILLASFSGQASLGMGDGYLLPSIAVVV
                     VGGTLITGGRGHYLGMLGGVLLLTALATLLAGTTLPFAVRDIIYGLVVLAAVLALRER
                     HA"
     gene            29021..29893
                     /locus_tag="P5558_02515"
     CDS             29021..29893
                     /locus_tag="P5558_02515"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY23215.1"
                     /translation="MRIEVLVDVKTTLGEGPLWDVDEQRLYWIDSFDGRVFRCTADGR
                     EVRAWDVPMKIGSMCLRKGGGAVVSLQRGFHFLDLASGEVTLIHDPEPDKPHNRLNDG
                     KVDRRGRFVAGSMDTQEAGPNGSLYRLDPDLSVTRLEDGIIVSNGPCWSPDGGTFYFT
                     DSWSGEISAYDYDLATGAIANKRTFARMEYQGAGFDGATVDAEGGVWSAVVYVGKLVR
                     YLPDGRVDRVIDMPVKKATSVNFGGPNLDILYVTSMAKPPLPRFPDDPQPRGSLFAIH
                     GLGIQGVPEPRFAG"
     gene            complement(29937..30128)
                     /locus_tag="P5558_02520"
     CDS             complement(29937..30128)
                     /locus_tag="P5558_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23216.1"
                     /translation="MVLVEAWENRVLTMAIYSLMAEFVTDWSGMMAFSMLASLPLVVA
                     FILLQKFMVQGMTAGALTS"
     gene            30245..30811
                     /locus_tag="P5558_02525"
     CDS             30245..30811
                     /locus_tag="P5558_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23217.1"
                     /translation="MPKAKDESRRRYPSEPLARAEVLQRLAPHFEFFEEVKAESANGD
                     VMRIDAVSQCRLTGWTFGWEFKRSHLFKSEFADAMRQGIHYRLSRIADARLPTHKGLQ
                     LPAIVLFPDWLGEHDDDVTNYGREAEGMRLVGAQFRVGTMRQSGDERFAFIMGQSAIW
                     HSASGWTKNAEGLLLGKRSLGSTRKNDR"
     gene            complement(30828..31694)
                     /locus_tag="P5558_02530"
     CDS             complement(30828..31694)
                     /locus_tag="P5558_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY23218.1"
                     /translation="MRQRSLAGGLGMDQRRLLLNAMSYGLLILAIVIVSLPILWMLTV
                     SVRPNLEVMKIPPSWIPAHFTLASYGKILTNPRYIVVFLNTIFISLFVTFLSLFLGAL
                     AAYALARFNFMGQRVVLMFLIITQMFPLILLCIPYFRVFVMLGLYDTRTSLIIVYLTF
                     TLPFCILMLRSYFMNISPAMEEAAMVDGCTRMGAIFRILVPVSYPAFIGAGLYAFLLA
                     WNEFLFAMVLVEAWENRVLTMAIYSLMAEFVTDWSGMMAFSMLASLPLVVAFILLQKF
                     MVQGMTAGALTS"
BASE COUNT         4670 a        11152 c        11091 g         4910 t
ORIGIN      
        1 ccggaagata tgcgtgtcga cggcgatggt ggcctcgccg aaggcctcgt tgagcacgac
       61 attggcggtc ttgcgcccca cgccgggcag cgcctcgaga gccgcccggt cgttcggcac
      121 ttggccgcca tgccgctcga ggagcaggcg gctgagctcg atcacgttcc tcgccttgtt
      181 gcggtagaga ccgatcgtcc tgatcgattc ccgcaccgcc tcttcgccga gctccagcat
      241 ggcggcgggt gtgtcggccc tggcgaagag ccgcgccgtc gccttgttga cgcccacatc
      301 cgtcgcctgg gcggagagga cgacggcgac caggagcgtg aacggatcgg cgtagtgaag
      361 ctcggtcgcg ggcgccggcc ggtcgcgcgc cagggtggcg aaaagctcca ggatggcggc
      421 ccggccgagc cgcgagcgag cggcaggccg gggcttcggc cggccggtgg gcgggcggcc
      481 gcctcgcgcg ctcttggcgt tgccgggctg gcgctgcata tttcccctga tgacgaaaga
      541 tggacgatga ccgacgacgc atccgcgggg acgggtccgg ccgccttcga ggcggtgctc
      601 tatcccaacc agccgccgcc gaccagcagc ctcgtgctcc tggtcgtggc ggtggccagc
      661 gtggcggtgg gcatgagcat cggcttctac ctcgccgggg cctggccggt ttccggcttc
      721 gtcgggctcg agctcggcct gctgatcgcg gcgctcctct ggacacggcg cggtgcccgc
      781 taccgcgagc atgtgcggct cgacgacacg ggcctgcatg tgcgggcgat cgccggcaat
      841 cgcgtgctca gggcgtggcg attcgagccc tattgggtgc gcgtcgggct cgagccggtg
      901 cggcatggcg aacgggtgct gcagctcagc gcccacggcc gcacgctgac gctcggccgc
      961 ttcctgacca gcgccgagcg ctgcgatttt gccgccgccc tgaaccgggc actcggccgg
     1021 tatcgggaaa atagcggctg agcgacctgt cggaattcct tacggatatc ccggacagac
     1081 caggcgaggc ttgccgatgc gcagaaggcc atccacaact gccgcccgcc ttggccgaag
     1141 ctcggacgac cgccgggcta ctgccgctgc tctgcgccgc cccccgagct tcccgcccga
     1201 gcggtcggcg agatctgact gattagctta cagatttccc agatcatccc cgcattctcc
     1261 tccggcgccc gggcaggagc tccggccagg ggttcttgtg cgttgatccg caacgttatt
     1321 ccacgtccgg catcgtgcct gcatcccacc ctcgagagag cctccaaggt gaagagcgga
     1381 tccgtcgatg aacgagcccc agcaaggtgt cccggaggcc ggcgcgcatc gtctgccggt
     1441 gagcaagctg cgcggcgtgc cgcagatggc gcgggtcagg ctcaagagct tgcgcatcac
     1501 cacatgcggg aaactgctgg cggcggcagc cacaccggcg ggccgggaac ggctggccgc
     1561 ggccacgggc atcgacccgg acctgctgct gcagctcgtg cggcgcgccg atctcgcccg
     1621 gatcaacggg atcggcacgg tcttcgggct gatgctggag gcgctgggcg tggacaatgt
     1681 cgcccggctg gcccgggagg atgcggcatc gctccaccag gcgctccgca gcttcaacaa
     1741 ggaggagcgg ctcgcccgcc gctcgcccac acccgaggag gtggccgagt ggatcgaggc
     1801 tgcgagggcc ctcccgccat gcgtggccga ggaaccggtc gagggcgact gaagggcagc
     1861 tgccgggctc gcacatcgac cctgttccat gagcagcggc aatcccgggg gccccggtcg
     1921 agcgatcgac gcggcttcgc ttgatcgacg tcaaggcatc ggcatgcagc ccggccttca
     1981 ttgccgggcg tcgttgccga cccgcttcct ggagatcgct catgccccgc ctgccgcacc
     2041 tgatggggta ctcgcgtgcg cagatccggc tgcactggct ggtcgccgtg ctgattaccc
     2101 tgcaatacct cctgcacgag ccgatcgtgg cggcctggcg ggctttccgc aagggtgaga
     2161 cccttgcctt ccagcccctc gtcgccctgc atgtcgccgg cggcctcgcc attctggcgc
     2221 tggtcctctg gcggctctgg ctgcgctacc ggcgcggcgc cccaccgccg cccgaggccg
     2281 agcccgcgcc cttgcgcctc gccgccaggg cggcccacgt cacgctctac gcgctcatgg
     2341 tcgcgatgcc gctttcaggc gccgcggcat ggttcggcgg gatcggggcg gccgccgccc
     2401 tgcacggctg gctccgcatc gtgctgctcg tgttgatcgg gctgcacgtg ctggccgtgc
     2461 tggcccatca gctcgtcttc aggacgagtt tgatgcagcg aatgtggcgg gcggagatct
     2521 gaccgccggc ccgccttcga ccgcggctcg cagaaccttg tacatgtagg tggtggcatc
     2581 gtagcccgag ccgtccgggg cgagcgagaa ggcggggaca cacccggtga cccgatagcc
     2641 gagcctgcga tagagccgct cggcggcgct gcccgtcgtg gtgtctaggg tgacgaggct
     2701 ccggccgtgc gccctggcct gcgcctcgag ctcgatcatc agccgctcgc cgatgccgca
     2761 tcttcgggaa tcggggtgga ccaggagctt ggtgacctcc gcgcgatgcg cctggttggg
     2821 cggcaggtcg aggatgagct gcaccgtgcc gacgatccgc tcgcggcacc gcgccacgag
     2881 caggatgcga ccgccgttcg gcagcgccgg gcggaccttc tccagccaga aggcgcgggc
     2941 gtcgtccggc gtgtagggca ggatgaagtt gacgctggcc ccgccgttca ccgcagcgtg
     3001 gagaatgccg ctgaggtcgt cgaggcaggc ggcgaagcgg tcgctgccga tcgcctcgat
     3061 ggtgatcgcg ttcatgtcag gctccgacca gaacgagaac gtagcgggcg cccgcctcgg
     3121 catcggcggc gaaggcgctc ggtcccgcca gccggtagcg caggcagtcg ccgggctcga
     3181 gggcgtggca ccggccatcg agggtgacca cgaggcgacc ttccagcagc acgaggtgat
     3241 gctcctggcc cggcacgggc ggggcagcat aggcgagatc ggcaccgggc cgcagcgtgc
     3301 cttcgatgat ctcggccgcg agcggtcctg ccggcggcga gacggcgcgc cggagaaaac
     3361 cggcctcgtc gtcgcgccag agcggctgtg cctcacgcct gacgacgggg gcaaaatccg
     3421 cctcgacagg ggccaggagc cgggtcagcg acaggccgtg cgccacgcag aggcggccga
     3481 gcacgtcggt ggtcgggctg acctcggcat tctccagccg cgacagggta gcccggctga
     3541 ctccggcgag cgcggccaga tggtcgagcg tccagccgcg ctcctggcgg agttgccgca
     3601 ggcgctcggc gaggcgggag gtgaggccgt cgtccagcac gaaattccat attcggaatt
     3661 attttccgaa tacggaaatc ccatgtgcgc gaatgattgt caagcccgcc cccgccgaac
     3721 ggttggtcag gcggccgtct tgatgttctg gttgaggctg aagaagttgg tcgggtcgta
     3781 tttccgcttg agctcggcga ggcggtcgta gttgctgccg aaggcggcac ggatgacgtc
     3841 cgggctttcc tcggacagga agttcagaag gaagccgccg ctcgagaaag gttgcatggc
     3901 gtcccaggcg gcgcgcgccc aggcggtatg ggtggcgtcc tcggccgggt ccgtccattg
     3961 cgccatgaag ccgatgtcgt actcggcctg gcgctgggcg aaggcggtct ccgccggccc
     4021 gacggcgctg gcggccccgg catagaactc gaccacgacg gcggagagcg gcgaggtcat
     4081 gcggttggcg tggccgacca gggtgtcgat cgcatcgtcg tcgagggcct tgacgaaggt
     4141 cgacttccag tagttgcggg tgccgtcctg gaaggcatcg tcgagcatcg actgcatgac
     4201 cgggaacggc atgggctgga cggcgtccat gagcgggctg ccgaactgcc ggagcggcgc
     4261 cacggcccgt tcgccctccg ccatgtcgcc gcaccagcag ggaatgacgg cgaccgccgg
     4321 atgcccgtcg ggcgtggtca gcagggcggc ataggcggtg agctcgagcg gggcggtctt
     4381 catgaagtcg cgataatagc gcagcacggc cccggcctgg tcgcgggcat ggacgatgag
     4441 cccgccgagc acggtcttca ccggatgcgc ctcgaaggtg aacgaggtga cgatgccgaa
     4501 attgccgccg ccgccccgaa gcgcccagaa gaggtctgga tgctccgtgg cgctgacggt
     4561 ccggtgctcg cccgaggcgt cgaccagctc gaaggccagc acgttgtcgc aggtgaagcc
     4621 gtgcttgcgc accagccagc cgaccccgcc gccgagcgtg agcccggcga taccggtcct
     4681 cgagaccacg ccggtcggca cggcgaggcc gtgcagatgg gtctcgcgat cgacgtcgcc
     4741 gagcgtcgcc ccgccctgga cgcgcacggt gcgcgaagcc gcgtccacca gcacgccgcg
     4801 catcagcgaa agatcgacga cgagcccacc gtcgcaaagc cccctcccgg caacattgtg
     4861 gccaccgcca cgcacggcga cgagcaggtc atgctgccgt gcgaaggtca ccgccgcgat
     4921 gacatcggcg gtaccgagcg ggcggacgac gaggccggga tgccggtcga tcgtcgcgtt
     4981 ccagatccgg cgggccgaat cgtagtcggc atccgcgggc cggatcaccc gaccggcgag
     5041 gccggctgcg aaagcgtcga tggcgtctgc tgcaatggcg atgccactgc ggttcacgag
     5101 cccggtggtc gacatggcgt gttccccgtt cttgcgctcg gctccttttt ttctcgctgg
     5161 gcgcattcta tcggaatgcc gcgtctcgcg ggagcgcgtt tcgcgtcggc ggcgtgctcg
     5221 gccatgggtc gggccctgcg aacgatgcat ccggcatctc accagcctga cttctgttgg
     5281 gagcggtttt acttggcatg agattggcgt ccaatatttc ggaccaccga agccctgcgg
     5341 gcaagatgtt cgaatgttcc ttgccacgcc ctcttcattg gcgacgtccc gaccacgatc
     5401 gaaagtggag cggcgagcgt cagggccaga ggcggctgtt gacagcatcg ggccggcggg
     5461 cgagaatgac tgtatgcttg ccccattcgt gccgcgcctg acgtcaatga cgaggttctg
     5521 tcccatcgca ggacattgac ccccggccgg ccgagtcgtg ctccggtcgt ggggcatcgg
     5581 cgatccagcc cgtcggtcgg gtcgcccggg acgcgacatc tgaaccagag cccaaatcga
     5641 acgtttgatc cggcatttgc cgtggcagga atcgtcgcca cggccatgcc ttgcacggag
     5701 gtccagagaa tggacgctga agcgaagaag catgccaagc ccgtcatcac gctcacgcag
     5761 tcggaccacg acagcctgac cggcatggcc cggaccaccc gaggcgaggt cgccgagcag
     5821 ttgttcgagg agctcgagcg ggcacgcgtg gtgcccgacc acaagctgcg cccgggcatc
     5881 gtccggatgg gctcgacgct gcgcttttcc accgacagcg gcgagtcccg cgaggtgacg
     5941 ctggtctatc cgggcgaggc cgacatcgcc gccgggaagg tttcggtcct caccccgatc
     6001 ggggccgccc tgatcggcct cgccgccgga cagtcgatcg actgggtcac ccgcgacggg
     6061 cgccgccggc ggctgaccgt cgagaacgtt gtggatgccc ggcccgcacc ggccgaggca
     6121 ccggcggcgg cggtgcagcc gacgccctga ggcgctgcgc aagcggcgcc ctgagccata
     6181 gcccccgatt gatggtgacg ctgccgacga tccggcgctc ggcatggctg ctgctcggcc
     6241 tcgtctgcgt cgcggtggcg gtcgtcggca tcttcctgcc gctggtgccg accacgccgc
     6301 tcctgctgct ggcggccttc tgcttcgcgc gcagctccga ccggctgctg cgctggctgc
     6361 acgagcatcc gcgcttcggc ccgaccctgc tgcattgggc ggagcaccgg gcgatcgcgc
     6421 agcgcgccaa ggtcatggcg gtgctggcga tggcagcggc gatcgggctg gcgatcgggc
     6481 tgggcgcgcc cttctgggtg gtgctggtgc aggtggcggt gctgctgccg gtggcgctct
     6541 tcatcgtcac gcggccggag ggcccgcgcg cctgacattc tgccgattgc cgggcggtgc
     6601 gttccggtct atcgactgag gaaacgggaa aaggggcggc agtcgatcga gagcaccgac
     6661 accgagccgg cgcgcatcct gatcgcgtcg agcaccggcg gctaccaggc gatcgacctc
     6721 tcggcccgcc tcgccgccaa ccttgcctat cgcctcgccg accatttcga tgtctcggcg
     6781 ggcgcgatcg ccgccgagga gcgacgtctt catcgctgcc ggatagcggc ggcgcagccg
     6841 ggagaaggac gatgaatatc cgacacacgc cgccgacggt ggcggcaccc ttcggcccct
     6901 acgcccatgg cgtcgaggtc ccgggagggt cgcggctgct ctacatctcc ggcgaggtgg
     6961 gcgtgctgcc cgatggcacg ctgccggacg gcatcgaggc gcaggccgac gcctgctggg
     7021 cgaacatcga ggccatcctc gccgacgccg ggatgggcgt cggcgatctg gtcaagatca
     7081 cgaccttcct ggtgcggccg gaggatctgg cggcggccgg ggcggtgcgg gcgaagcatc
     7141 tgggcgaggt gcggccgggc tcgaccacga tcgtcgtcga ggcgctggtc aggccggaat
     7201 ggctgatcga gatcgaggcg gtagcggcgg cgaagggctg aacagatggc tgaattcaag
     7261 gtcacctatt tcgacatggc cggcagccgg ggcgaggatg ttcgcctggc gctggtgatc
     7321 gccgggcgga cgttcgagga catccgcgtc gaccgcgagg cgttcttgaa gctgaagccc
     7381 gagctgccgt tcggctcgct gccggtgctc gaggtcgagg gccacggggt cttcagccag
     7441 accaatgcga tcctgcggct cattggccgg ctctgcgacc tctatccgca ggagccgttc
     7501 gaggccgccc ggcacgacgc gttgatggac gcggtcgagg atctccgcca ccggatcggg
     7561 ccaacggcgc gcatccagga cgccgcggcg aagaaggcgg cacggcagca gctcgccacg
     7621 gacctcatcc cgcgctgggg cgagggtgtc gagcgtctga tcggccaggg gccgttcgtc
     7681 ggcggcgagc ggccgggcgt cgccgatatc aagctctaca tggcgcatcg gtggctcggc
     7741 agcggggtgc tcgacgatat cccgaaggat gtgctcgatc actgtccgaa gctcgaggct
     7801 gtcgccaacg ggatcgcgac gcacccggcg gtccgcgact ggtacgcgaa agcgggctga
     7861 agggcgcccc gttcggccga tgtcttggcc atcaccgcca gcggtctggc ggtgtcgcca
     7921 acgctccgct aggcaggcgg gatgaccgcc gaacgcacga tccgccgcct cgccgccatc
     7981 ctcgtcgcgg atgtggtcgg ctactcgcgc ctcatggccg aggacgagac cgcagcactt
     8041 gcagcgctga accggcaccg cgaggtcgag ttcgatccga tcgtggcgcg gcacaatggc
     8101 cggatcgtca agctgatggg cgacggcacg ctcgtcgagt tcaggagcgt cgtcgacgcc
     8161 gtcacctgcg ccgtcgccat ccagaccgcg gccgtcgcac ccggcagcag cccggcgatg
     8221 atgctccgga tcggcgtcca tctcggcgac atcatcatcc agggcgacga catctacggc
     8281 gacggcgtca acatcgcagc ccggctggag ccgcgggcag cagccggtgg ggtgtgcgtc
     8341 tcgtcggtcg tcaacgaaag cctgcgcgat cgcgtcgaca ccgtcttcgt cgacggcggc
     8401 gacgttgcgg tgaagaacct cgcccggccg gtcagggtat ggcactggca tcccggggac
     8461 ggatcggcgc cggcgcccgc cgtgcccccg cccgcacggg caagcgaggg tcgatcgatc
     8521 gccgtcctgg cgttcgccaa catgtcgggc gacgccgagc aggagtactt ctccgacggc
     8581 atcgccgagg acatcatcac cgatctctcc aaggtggccg gcctcctggt gatcgcccgc
     8641 aattccagct tcgcctacaa gggccggtcg gtcgatctgc gcgacgtcgg ccgcgagctg
     8701 ggtgtcgggc acgtgctcga gggcagcgtg cgccgtgccg gcggccgggt gcggatcacg
     8761 gcgcagttga tcgacaccgc caccggcggc catctctggg ccgaccgcta cgaccgcgag
     8821 ctcaccgatg tcttcgccgt ccaggacgag gtcacgctga agatcgtcga cgccctcagg
     8881 gtcaggctct cgcccgccga gcgggccgac atcgtcgccg ccggcacgac cagcatggaa
     8941 gcgcatgact gcttcctgcg catgcgcaac ctgatcttct cgccgggcct gacccccgcc
     9001 ctctggcagc gcggcatcgc cgaggggctg cgcgccatcg agctggaccc gacctacgtg
     9061 caggcccggg cgctcacctc gatctgccac tggctggact tccacaacgg ctggtcgggc
     9121 aatgcgccgg aggtcgtggc ggccagggcc cgcgcactcg ccgaccgggc catcgagctc
     9181 gatccggcgg acccgttcgc caaccacgcg atcgccgtcg ccgcaaggtg gcagggcgac
     9241 tacgacctcg cggccgaggc catcgaccgg acgctggcga aaaaccccga ctatgcgctc
     9301 ggcctcttca cccgcagcga gattgcgctc gccgcagggc ggcacaccga tgcgatcgcc
     9361 gatctcgagc acgcgacgcg cgtcgatccc gcctggaccc accagtatct gcagaacatg
     9421 ggcatggcgc atctgctgct cggccatgtc gagaccgccg ccctgatcct ccgcgaacgc
     9481 ctgctcctgg ccagggatac cgatatcggc cgtgcctggc tcgcctcggc gctgggccat
     9541 ctgggcgaga tcgaggaggc gcggcgcctg tgggccgagc tgctcgagat caacccctcc
     9601 ttctcgatcg agcaacgcct ggcccggcag aggttcgcgc ggcgggccga tatcgaccag
     9661 gtgatggcag gcctggccag ggccgggctg ccagtcggac catagaggtc gtcttgcctg
     9721 ccgcgatgac tggtcaaggt cgatcgaaag catcaattga cacaaccagg gacgccccca
     9781 taactggcgg ttgccagtcg gcaacaaatg gagatgacca tgaccaagat cgcactcccg
     9841 cttgttctcg ggctcgggct gaccatcggc ctcgcccagg tgcaggatgc ccacgccgac
     9901 agcacgggct gcggtctcgg cacgaccgtc tggaagggcg aatccggcgt cggcccccag
     9961 gtcctggcgg tcaccaccaa cggcacgtcg ggcaaccaga ccttcggcat cacgtcgggc
    10021 acgctcggct gcgagcagaa tgcgacgatc gcctcggtcg aggtcaggaa cctcaccgtc
    10081 gccaatctcg acaatctggc cacggacatg gctcggggcg aaggcgagac gctgacctcg
    10141 ctcgcggttg ccatgcaggt cgagccccag gaccagcccg tcctcttcgc caccttccag
    10201 cagaatttca cgcggatctt cccgtccgag gatacctcgg cggaggacgt gctcgtcgcg
    10261 atgcgcgagg tgatgaccga gaacgagacg ctggtgcgct acgtcgaggt ctgacgtccc
    10321 tcgcaagctg tagcccggca tccggcggca gcccgatgcc gggctcggtg ataccgggcc
    10381 ggatgggatg accgagcgcc atcgatccgg cccgacgctt ttttgtgctg cgcccatgag
    10441 gcccgtcccg ctgctgctgg ttctgctggc gccgtggtgc gggagcccgg cggtgccggc
    10501 cgagccggcc tatctcgacg agctggtcgc gagcgcggag gcccagggtc tcgccaccgc
    10561 accgggctgg gaaacgctcg gccactaccg cccgaatttg ctgggccggg gtcgcaagag
    10621 cctcatcgac agcgagagct tcttcctcgc gaaggacggc aagagcgacc ccgcgagcga
    10681 gctcgaggcg acgctcgccg ccttcttcgc cccggccggc gagccggccc tgcagcatcc
    10741 gcaatgccgc ttcgtcgccc gctaccggtg gctgaaggac cggctgggtt tcgatcccga
    10801 ccgcctgccc gagcaggatt gcgccgagtt cgacgcctgg ctcgcgacca tcgacgccac
    10861 cagcgtgacg ctgatctttc cggcggccta tctcaacaac ccggcctcga tgttcggcca
    10921 cacgctcctg cgcttcgacc ggccggacca gaccgaagag acgcggctcc tctcccacgc
    10981 ggtcaattac ggtgcctgga ccgggaccga caacggtgtg ctcttcgccg tgctgggcct
    11041 caccggcggc tatgagggga cctactcggt cgagccctat tacgagctgg tcaagcgcta
    11101 cagcgacctc gagaaccgcg acatctggga gtaccagctc gatttctcgc cgaccgaggt
    11161 ggagaggatg ctcgcgcatc tgtgggagct gcgggaccag cacagcgact actatttctt
    11221 cgacgagaac tgctcctacc agctcctctt cctcctcgac gccgcccggc cgaagctcga
    11281 cctcgtcggt gatttcgacc tgcacgtcat cccggccgac agcgtgcgct cggtggtgcg
    11341 ccgcgagggc ctgctccggc acacggtgtt cagaccctcg ggcgagacgc ggctgcagca
    11401 gggcctcgtc gccatgcccc cggccgaccg gaaattggtg cgcgggctcg cctcgggggc
    11461 gatctcgccc ggggaggcgg ccgcgtcgaa catgtccccg ccccgcctcg ccgccgtgct
    11521 cgagctcgcc gaggcctcgg tcacccacga gctccgcgcc ggcgatctcg agcgcgacgt
    11581 cgccgccccg cgcgcctggt cgctgctcgc cgcccggagc gaaatcgaca cgccggccga
    11641 tcggccgccc gtgcccgtgc cggcgacccg cccggaccag ggccacggct cggcccgtct
    11701 cgccgccggc atcggcgtgc gcgacggcga tgtcttccag gccctgcgcc tccgccccgc
    11761 ctatcacgac ggcctggacc ccgccggcgg cttcgcccgg ggcgccggca tcgaggcgct
    11821 cgacctcgag cttcgccatt accagggcga caactcggtg gtgctcgatc gcttcaccgc
    11881 cgtccgcatc agctcgatgg cgccgcggga cgagctgatc cggcccgtct cctggcggct
    11941 cgatttcggc ctcgagcgga tgcgggtcga gcggggcgac accaagggtg cgctggtcgg
    12001 tgtcgccgag ggcggggccg ggctcggcta cgggctcggg cagcagggcg tctggtccgc
    12061 catggcggat atcggcctca gcggcggcga ggactgcgac cggacctgca gcgtcaatct
    12121 cggccccgcc ttgtcgctgc tctggccgat caccgaccgg gccaccttca ccgccagcgg
    12181 cagctaccgg gtccgcttcg gcgagcgcgt ccgcgacggc tacgagcttc gcctcggcca
    12241 gagctacggc ctcgccgccg atctcgcgat caaggtcgag ctggcgctgg agaaccaggg
    12301 cggcggcatg cagccggagc tcctctccac cctcaactgg tacttctgat gactcgggtc
    12361 cggtcgctcg ccgccctcgc tttgctcctg gcgctcggcg cctgcaccgg gctcttcttc
    12421 cagccgatga agagccacgt cttcgatccg gcgagcatcg gcttcaccta ccgcgacgtc
    12481 gacttcgagg cggtcgacgg cacggccctc cacggctggt acttcccggc cgagggcgag
    12541 ccttcgggca gcatcctctt cctccacggc aatgccgaga acatcagcac gcatttcgcc
    12601 aatgtcgcct ggctcgcgaa gtccggcttc gccgtcctcg tcttcgacta tcgcggctat
    12661 ggccgctccg ccggcagccc cgatctcggc ggcctgcacc tcgacgtcgc cgccgccctt
    12721 gatgcgctcc tgcggcagcc ggagaccgat ccggaccgca tcgccgtcat cggccagagc
    12781 ctcggcgcag cactggcgct gaccgccatg gccgcgagcc cgatgaagga ccggctgcgg
    12841 gcggtgatca tcgagggtgc cttcgtcggc tacccggaga tcgcccgcga gaagctcgcc
    12901 gccttctggc tcacctggcc gctgcagcag ccgctaagct ggaccatcga cggccgctac
    12961 agcccactcg aggcggcggc cgaaattgca cccgtgccac tcctggtcat cctcgacgag
    13021 gccgacacca tcatcccgcc acatcacggc gaggcgctgt ttgcagcggc cggcgagccg
    13081 aagaccctct ggcgcctgcc gggatcgggc cacatccagg ccttagccac ggtcggcaac
    13141 cggcatcggc tggtcgagta tctgcaagct gtcatggcgg cgccgccgat gccgacccct
    13201 tgaaccggct tcgctagccg atcgtctcga cgggcccgcc ggccgaccgc caggcgcgaa
    13261 agccgcccgg gatgtgggcc gtgttcctga cgccgtgctc ggcggcgatc tcgaccgcca
    13321 gggtcgatcg ctcgccgacc gcgcaataaa gcacgaggcg cgcatcccgg cccagtcgcc
    13381 ggagcggccc ggacgcgtcg caagacgagg cgaactggcc atagggcagg tgcatggagc
    13441 ccgggatcgc cccgtcgcgc cgccgctcgc tctcctcgcg aatgtcgatg agctgcccgt
    13501 cgacctcggg ccaggtggcg agcagcgcct cgacctcgag caccggcacg cggtgctgcg
    13561 cctcgagctg gagcccgacc cgcaggttct ccggcaccgc gacatccatc atcttcgggt
    13621 tcggcaggtt caggttgttc atgatctctg cgtattcgtc ggccgagccg accttgaggc
    13681 gcgggttgtg ggccttctcc tcggcgatgg tgctgaccgt gtcgcccttg tagtcgtgcg
    13741 ccgggtagac gagcgtctgg tccggcagct tcagcaggcg atcgaacaag gagcggtact
    13801 ggtcgtaggg atcgccgttc tggaaatcgg tgcggccggt gccccggatc aagagcgtgt
    13861 cgccggtgaa gacccgatca tccatcaaaa agctgtagct ctcgctggta tggcccggcg
    13921 tgtgcagggc ggtgagcgct atcccctcga tggtcagccg ctcgttgtcg ctcacccgca
    13981 tcgagaccac gtcggccggc gactgctcgc ccatcaccgt gacgcagcgc gtggcgtccc
    14041 gaagccggcc catggcgctc acgtgatcgg cgtggatatg cgtgtcgatc accttgacga
    14101 gcttcaggtc gagctcctcg agcagcttga gataccgccc ggtctgctcc agcaccgggt
    14161 cgatgagcag cgcctccccg cccgcccggc tcgccatcag gtaggtgtag gtgctcgatg
    14221 tcttgtcgaa gagttggcga aagagcatgg cgttttcccc ttctggcatc ggccggccga
    14281 ggcattgcag cacaggccgg cgctccgtcc gcggcgccat ctcgtgcgcc ccggcctcga
    14341 agatggtatg cgaggtgccg acgcagcaag gccggccgtt ggcccgggcg gcattcgagg
    14401 tgatttctgc atgccgatca cggttctcgc cgagacgcgc ctcatcgaga tcgcggcacc
    14461 gttcgtcttc ctcgtttgct ggatcggcta ctgggccttc gccgaccagc tccagctcgg
    14521 caatcgctcg ctgatgcggc gcatgcacga ataccggcga gcctggctca tgcagatgct
    14581 ggtgcgtgac aaccggatcg tcgacatcca ggtggtccag gtgctggtgc agaacgtctc
    14641 gttcttcgcc tcgtccgcca tcctcatcgt cggcggcttc gtcgccgtgc tcggggcgag
    14701 cgagcaggcg cgggcgatcg tcgccgagat acccttcgcc gaggacccga gctcgctgat
    14761 ctgggatctc aagatcctgc tgctgatcgt gatcttcgtc tacgccttct tcaagttcac
    14821 ctggtcgctc cgcctcttca actacgtggc catcctgatc ggcgccgcgc ccgccggccg
    14881 gtcgcccgac accatcgcca tggccgagca gatcgccgtg gtggccgatc gcgccagcga
    14941 ccatttcaac cgggcgatgc gcgccttcta tttcggcctc gccgccctcg cctggttcat
    15001 ccagtcctgg ctcttcatcc tcgtctcggt cctggtcgtg gtgatcatct accggcgcga
    15061 ataccgctcg gccgtgctca ccgtgctcgg ccccgtcggc cggtcgatcc ccggggcacc
    15121 cgaggaaggc taggcggggt attcgagggc gccgagaatg tcggccgcga tctcgaagga
    15181 gcgcagccgg gcggcgtggt cgtggatctg gccggtcagg atcagctcgt ccggccggta
    15241 gcgggcgatg aggtcctgga ggccgcggcg gaccgtgtcg ggtgcgccga ccgcggtgat
    15301 ggcgagcgcc tgctcggccg aggccagggc aacggggtcg aggtgccgct ccacgtcctc
    15361 caccgggcgc ggcagcgggc ccgcgcgccc cgtgcgcagg ttgacgaagg cctgcagcat
    15421 ggagctgcgg aggtaatgcc cctccgcgtc gctggcggcg gcgaagacat tgagggcgag
    15481 catgaagcgc ggctgctgca gccgtgccga gggccggaag gtctggcgat agacggcagc
    15541 cgcctggtcg agcgctgccg gggcgaagtg cgaggcgaag gcatagggca gcccgagatg
    15601 ggccgcgagc tgggcaccgt agaggctgga gcccaggatc cagatcggca cctcggtgcc
    15661 cgtgccgggg ttcgccttga cgatctggcc gggctgctcc gggccgagat aggccatgag
    15721 ctccatcacg tcgcgcggga agtcgtcgcc ggcatcgaga tgacggcgaa gcgcccgcgc
    15781 cgtcgccatg tcggtgccgg gtgcccgccc gaggccgaga tcgatccggc ccggatgcag
    15841 ggtggccagc gtgccgaact gctcggcgat gacgagcggc gcatggttcg gcagcatgat
    15901 gccgccggcg cccacgcgaa tccgcgtcgt gccggcggcg acgtggccga tcaggacggc
    15961 ggtggcggcg ctggcgatgc ccggcatgtt gtgatgctcg gcgagccaga agcggtgata
    16021 gccccagcgc tccgcgtgct gcgccaggtc gagcgagttc cgcaaggcgt cgctggcgtt
    16081 gctgccctgg gcgatcggcg ccagatcgag gaccgagaag gggatcatgt gcccgggctg
    16141 accgtggcgg ggcgccagcc gaggcggcgg cgggcccgct cgacgaggcg cgggcggtcg
    16201 agcacgctgg catcgatggt catgccacga ttgccggcgc ggatattggc gaagaactgg
    16261 ccagcgctgg cctcccagga gaagcccagg gcatgcgcgc ggcagcgctc gcgcgggata
    16321 tccagggccc cgagcgccgc ccgacgcagg tcgtgatcga gcacgcccgt gccggacgag
    16381 ccgacgatgg tgagcggccc ggcggtcggg taggcggcga tcggcaggcc cgaggcgagc
    16441 gcctcgagca gcaccatgcc gaaggtgtcg gtgcggctgg gaaagacgaa gacgtcggcg
    16501 gaggcgtagg cccgggcgag ctccaggccc gtgagcttgc cgaggaagcg ggcatcgggg
    16561 aaggccgcct cgagctggcg gcgcgccggg ccgtcgccga ccacgagctt ggtgcccggc
    16621 aggtcgagcc tgaggaagtc gtcgacgctc ttctcgatcg ccagccggcc gacatagagg
    16681 aagaccggcc cctcgcagcc gagccccgtg cgccgggccg ggtcgaactg cgccgtgtcg
    16741 acgccgcggc tccagcggat gatgttggcg aagccgtgcc ggtcgagctc ctgggcgagc
    16801 gaatcggtgg cgaccatgac gccattgccg ccattgtgga accagcgcat cagccgccag
    16861 gtccagtcgg tcgggatcgg caggcgggcg cggacgtagt ccggcaggcg ggtgtggacg
    16921 caggttgtga acggcaggcc gtggcggaca cagagacgcc gggtcaggat gccgagcggc
    16981 ccctcggtgg cgatgtgaac atgcgtcggt gccagccgcg cgatcagccg ggcgagccgg
    17041 ccgggccgcg ccagcgccag ccggatctcg ggatagctcg gcagcggcac cgagcggaag
    17101 tgctggggcg tgaggagggc aatctcggcg ccgcagccgg cggcgagctc catcatccgt
    17161 tcgatcgagc ggaccacgcc gttgagctgc gggtaccagg catcggtcac gatcagcagc
    17221 cgcatggcgt catcagccgc ggtctcgtcg cccgcattcg acttggtcaa gtcttccccc
    17281 gtccgcgccg ctcgtcacgg cgctgcgtat tgatccctcg cgaggagata gtccctgcca
    17341 gccgaattgg ccatgcgacg caaccgttca ggcgcggcaa tcctcgagac gaaggtagcg
    17401 ggcgtcgagc ccgagatagt cctccttgcg gcaggtcagg accaggatct gctggcgggc
    17461 cgccgcctgc tgcaggatgg tcttcatccg ggcgagccgc accgcgtccg cgtagacgag
    17521 cgcgtcgtcg aggatcaaag gcgggctctg gccccgcgtc tccaccagga gatcgccgat
    17581 cgccagccgg acgaggatcg cgagctgctc gcgcgtgccg acgctgaggc tggagaaagg
    17641 ctcgctcgcg gcgctgcgcc tgagcgtttc gagccgcagg ctctctggcg cgaaggcggc
    17701 caccgcctcg cccatcaact gccgcatgta gggcgcgagg cgtgcctcga gcggctcgac
    17761 cagcgcatcc tgccgggccc ggtcgaccgc ctccagtgtc tcggccagga gccgccaggc
    17821 ccgggcctcg gcaaagaggc gggcatggcg ccgctcggcc agctcgagcc tgccctgcac
    17881 gtcccccttg cggtcgccga tgccgcggcc gccggcgccc ttcagctcgg cctcgagccg
    17941 gaccacctcg cgctcgaggc cgcgcccctc ctcctcgagc tgggcgaggc gacgcgccgc
    18001 ctgctccgcc tcgagccggg ccgcctccgg cgccgcccgg gccagctcgc gctcgacccg
    18061 cagcaccgcc tccgcggccc ggtcgtcgac cgtccgggcc gccgccagcg cctcggcgag
    18121 ggcgacatcg gccagctcgt cccgctcgat cgcgagctgg tcctcgagct tggccagctc
    18181 gagcccggcc cgcgcctcgg cacccgccag cacgtccgcc tcccggccga gccgggcctc
    18241 ctcctcggtg gcccgccgtc gttcctccgc cagcgccgcc tcgcgttcgc gcagctcggc
    18301 cagtgtccgc tcgacagcct cgcgctcggc ctcgagcgtc gcatcgtcgg catcatcgcc
    18361 gccatcgaca tcgtcgaagc cggcgagccg tgcccgggcc gtgacgcagg cggcatcgag
    18421 tgccgcctgg cccttcatcc cgctcgcccg gagcagagtg gcgagcttct gccgcgccgc
    18481 ctcggcctcg tgcgcgtggc ggtccctggc cagcagccga cggcgcgcct cggcgagatc
    18541 ccgggcaacg agttccgcga gccgtccggc gaatgctgcc tcgccatcgg cgagcgcccg
    18601 gcgcagctcg gccagcccct tgccacccgg tgtcacctcg atccggccga agccgggaag
    18661 gatcagggtc gccggtgccg tgagctcgat cggcgcagcc ggatcgaccg gcaccaggcc
    18721 cagcagcacc tcggcatccg cccgatcggg ccgcagcacg agccgcgtcg ccacggcacg
    18781 cagggcagcc cgcgtctcct tgagctcgac ctccatcgtc tcgagctgct ccagcgcgat
    18841 ctcgtcgatc gcctccgcgt cggcgcgggc ctgctgctcg gccgcctcgg ccgccgtcgc
    18901 ccggacatcc gccgcgaggc gctccagggc ggcgagctcc cgtgccagcc gcaaccgctc
    18961 ggcccgccgg cgcacggtct cccgttcgcg ctcgcccgcc gcaagcgccc tgcggctcgc
    19021 ctcggcggcc gcatccgtcg ccgcccgctg ccgtcgggcc cgatccagaa gcggggcgtt
    19081 ctcggcggtt gccctggcga gcttcgcctg ctcggcggcc cgctcgtcga tggctgccag
    19141 acgctgccgg cgcaggtccg ccatgtcctg cagccttcgc agctcgctcg ccgccgcctg
    19201 gcgctcggcc gagcgaaccc ttttttcctc ctcgagcgcc cgcacgccgg cgagggccga
    19261 gcggctgccg tcgacggccc ggcgcgcacg ccccagatga tcggcggcga gccaggcccg
    19321 ccgcttctcg agggtcgccg ccagccgatc gagcagcgcc tcgacctccg cctcggccgc
    19381 ttccagaccc gaaagctcga cgcgcagctc cgccacctcg cgctcgagcg tggcgagctc
    19441 gcccctcggc tggtcccgct tgccggtcca gtgctggtcg cgccgccgcc gcacctcggt
    19501 catcaggcgc ggcgcccgct cgccccgacc gagtgccgcc acctggtcgg ccagcgccgg
    19561 ctggagcccg cgcaaagccg tcgggctcgg ttcctgctcc tttgcgaagg tggtgccctg
    19621 gtcgacccag aagagcccgg cgacgccggt atgcgtgtcc cgggccgccc ccctgcccgg
    19681 ccattcgaac tgcaggagcg aggcgagccg gagctcggcc gcgtcaccct cgagcaagcc
    19741 ttccggcgtc tcgagctgga cgccgccccg gcgaaagcgc ttggccaggc gataggcgct
    19801 gccgtccagg gtgaagccga cggtcacggc cggcgtctcg ccgccggcga agggctggat
    19861 ggcgtcgcga tccgccccgg tcgtattgtg cttcatgaag aaggcggcct tcagcgcgtc
    19921 gagcagggtc gacttgcctt cctcgttgtc gccagagacg acggtgatgc cgagggctgc
    19981 cgggcgcacc acatggcggc ccttcagctt cttgaagtcc tcgacgacga gctcgtccag
    20041 gcgcatcagc cgggctccag ccgctgccac gtctggtgca gcaggcgaag cgccacgggt
    20101 gctgccggat cctccgagtc ggcgagcgct gccaccacct cggcgagcaa gggctcgctc
    20161 gccatggcgg cgagatcggc ttcgctggcg gcggtcagca ggccttcgtc ctggacctcg
    20221 aggtggcgca gccgcgcctc gagccgcccc agcgccgcct cgagcgccgc ccgcccggcg
    20281 agatcgaggg ccccttcgag cgtcaggcgg agaaccgtct cgccaggggc cgcgagaccc
    20341 gccaccaggg cgtcgagctg gtcgccgata gcccccacgg ccgggccgtg gccgagatcc
    20401 accttgcggg cctgccaggc gtgccgcccc gtctcgaggg cgcggaccgt caccggctga
    20461 cccgggccgg gcagcgtcac cagcaacgcc tggccctggc cgctccgggt gaagtcgtcg
    20521 ggctccggcg tgcccgagaa ccaggtgcgt ggccccacct ggaggaccga atgccagtcg
    20581 ccgagggcga gatagtcgag ccctgcccgc tcggcgcggt cgagcgcgat cgggttcggg
    20641 ctctccgact ggccgggcag cagcgccggc acaccgccat gcgccagccc gaccttgacc
    20701 gccccggccg gcaggaccgc cgtgtcgaac cacgccgtcg tgtcgccgct gacatgcttc
    20761 tgcagaagcg gggccggcag gacgaccagc cggccatcga ggagcgtcat tggctccggc
    20821 cgggtcagca ggcggacatt ctccgggcag tcgccggagg cggcaaagcg gtcccagagg
    20881 ccgcccggcc gcgccgggtc gtgattgccg ggcaaaagga gccaggggcc ggggaagccg
    20941 gccatccggt gcaccatccg ccgcagcagg ccgggctcgg catgctcgtg gtcgagcacg
    21001 tcgccggcga ccagcacggc tgccacatcg gcttcggtgg cacgagcggc gagacgctcg
    21061 atgacggcga gccgcgcctc gcgcagcagg gccgccaggt cgccgccggc gaagcgcgcg
    21121 aaggacttgc cgagctgcca gtcggcggtg tgcaggaggc ggatggtcat ggcgccgatg
    21181 gtgccacgcc gctggcggac ggatcaaccg tcgcgcgaca ggccctgtcg ttcgatctcg
    21241 gcgaggtagg cggcccagat cgtcgcctgc tccgccccca gccgatagag ccagctccag
    21301 ctgtagatgc cggtcgagtg cccgtcgtcg aagacgatgc gcacggcata gttgccgacc
    21361 ggctcgagcc gcacgatgcc gacccggcgc ttgccgccca ccgtcacctt ctgcgacggc
    21421 gtgtgaccct ggacctcggc ggacgggctt tcgacgcgca agagctcggc cgggtagctg
    21481 aagctccggc cgtcgtcgaa atcgatgccg aggcgccgct cctcccggtg gacgcggatc
    21541 tcgaccggcc aggcctcggc ctcactcatt gccgtgatcg tccttgtcgg cgcggaccgc
    21601 gtccgggatg cgatcggaat cgtcgagctg gcgggcatcg tcgaccagca tgatcggaat
    21661 gccgtcgcgc accggatagg ccaggcccgc ctgctcgctg accagctcgt tccgggcgcg
    21721 gtcgtagcgc agcggcccct tggtcagggg gcagatcagg aggtccagga gcgccgggtc
    21781 gatggcggcg ccactcgtcg gggtctcggg cgtgtcggtc atcgttgcgc tctccggaaa
    21841 ggcggcgggt cagtgcagga ccgagggctc ggcggcaccc gtggtccccg ccatgcacat
    21901 gagggcgatc aagagctccg cctgggcgtt caggttcgcg gcctcgagca aggcctgctt
    21961 ctcgttcggc tcgaacgggc agatcatcac cagcgaggtg acgaggccgg cgagcggcgc
    22021 atccttgatc gtctcccagt cggcctcgat ctgctggcgc tcgaaatagc ccttgagcgc
    22081 cgtcaagaga tcgccgcgca gggcgtcggg cggcggctcg ggctgcaggt cgtcgtgcca
    22141 gcggtcgaac gcggccacga cctggcggta cggcgtcgtc accgccagct cctcggcgac
    22201 gtcgaagcgg gcgatgcccg tcagcgtgat gaggaagcgg ccgtcctcgg tctcgcggaa
    22261 cgagctgatc cgcccggcgc agcccgtccc gtagatgggc ggctggccct ccgccgcctc
    22321 gccgcccccg gcttggcctc tgggctggat catgccgatc accttgtcgg tctgcatggc
    22381 gtcccgcacc atctgcaggt agcgcggctc gaagatgttg agcggcaggt tgccgcccgg
    22441 cagcaggacg gcacccggca aggggaagat cgggaaccgg ccgggcaggc tttcgaggcc
    22501 gggcatgctc aacctcgctc ccggctcagc tgaagaggac ggacgacagc agccggcgac
    22561 ccttgatggt cagcggatgg gtcgggccga gcgcttcgaa gaaccgcacc agctgctttc
    22621 tcgctccgtc gtcgcgccat tcccggtccc gcttcacgat ctggcggaga tgctccatcg
    22681 cctgctcgac ctggccgcgc aggaagagcg cggtcgccag atcgtagcgg gcgtcgtgat
    22741 cgtccgcatt gccctcgata cgccgggcca gctccgccgg atcaccgagc gtgccggcct
    22801 ccttggcgag cgccacggcc gacttcgcac cggtgacggc ggcatgcttg gcggccgcgg
    22861 ccggcacgtc gcccaaaagc tgctcggcct cggcgagatc gcccgcggcg agcaggcagc
    22921 gggcgaggcc gcccagcgcc tcgggattct ccggctcctg ctccagcacg ccgccgaaga
    22981 gctcgccggc ggcgtcgagc tcgcccgcct cgagcgccgc cttggcctcc tcgagctgga
    23041 catcgacgct ggccgggccg agcgggccgc cggtcagctt ctcgaccagc gccttgatct
    23101 ggctctcggg ctgcgcaccg gcgaagcccg tcaccggctg gccctgggcg aaggcgtaga
    23161 ccgtcggcac cgactgcacc cggagctgct gggcgagctc ggggttctgg tcgatgtcga
    23221 tcttgaccag cttgaccttg ccgccggcct gcttgaccac cttctcgagc accggggtga
    23281 gctgcttgca ggggccgcac caggtcgccc agaaatcgac cagcaccggc tggcgcatgg
    23341 attcctggat gacgtcgcgg gcgaaggatt cgaggttgcc gtccttgatc aggtccgcgc
    23401 caccaccctg ctgcccggcc ggagcctgcg tgccgccaat catctgcatg tcgaaaaaac
    23461 ccttgctgcc cgcaattcgc gcccacccga tggccggacg ccgacgccct cccgccgacc
    23521 gcttccgggt accacgggtc aggcatagat ggaaaccgga cgccggcgat gcaaggcgcg
    23581 ccgcgggcca cgcgcaagcc gcttgccaaa ccgcccccca tccactaaat gccgcgcctg
    23641 tgccggaaac gcttggcgga tccgggcgcg agcgggcgta gctcaggggt agagcacaac
    23701 cttgccaagg ttggggtcga gggttcgaat cccttcgccc gctccagatt tcctccgaga
    23761 gatcgagaag tcgaagagcg ccccaggggg cgctttttat ctttccgggc cttttccgct
    23821 accgtcgcct tccactcttc ccaccgagcc gaccagcgac gccaccacgc gcgctgatgc
    23881 ctgtcgatcg gcggcgggcg actacggcct ctagcgccag cactcgacca gcacggtgta
    23941 ttccttggtg cgggtgccga catcggcggc cgtcaggggc tcggggcatc cggcggtggg
    24001 gctggcttgg tgccgcatgg tccatcgagc tccggcaata tcggcgccct cgaaagtgca
    24061 tgtgaaggcc ggggcgtcgc gccattcgcc ggcgctgaac gcgattgtca cgatttcgga
    24121 tgtgtgatct atcatcgagg cgcagggaac ggcagaggcc gcccgggagg cggcccagtt
    24181 gggaggtcca ggagatgcgg ttgggatggc tggcggcggg tgtcgccatg gtcgggctga
    24241 tgggcagcga cgcggtcgcg gcggacaagt acaagatctt cctgtcgatg agctacatcg
    24301 gcaacgactg gcaggccgag gccgccaaca tggtcaaggc gatggccgac catgcctcga
    24361 tgcgcgacaa ggtcgacctg cagatccagg tcgccggccc gaacgcgcag cgccagatcc
    24421 agcagatcaa cgcgatggtg cagcagggtg cggacgccat catcgtctac ccgatctcgc
    24481 cgaccgccct gaaccaggtg gtcaagaacg cctgcgacaa gggtgtcctg gtcttcgcct
    24541 acgattcggt catcaccgag ccctgcgcct acaacaccac gatcgaccag gacgaggcgg
    24601 gccgcaagcc ggcggaatgg ctggtcgacc agctcggcgg cgagggcaag atcgtcgtca
    24661 tcaccggcgt ccccggcacc tcggtcgaca ccgcgcgcac ggcggccgcc aaggacgtct
    24721 tcgccaagca cccgggcatc gagatcgtcg ccgagggcaa cggcatgtgg agccaggcgg
    24781 tggcacggac cgagctctcc aagatcgtcg ccacccatcc ctggtccgag atcgacgggc
    24841 tctggatgca ggcgggctgc ttcaccgccg cgtccatgca ggacgaggcc gggatcgccg
    24901 acgcggacaa gaagccctgc gccggcgagg gctccaacgg ccatcgcatc cagatgctgc
    24961 cgatcgggac cgaggggatc gagggtgcga acggcaccta tcggccgatg ggctacccca
    25021 gcatctccta cgcctcgccg ccctattcgg gcgcgctggc gctgaagaac gcggtcgcca
    25081 agctcgaggg caaggagatc ccgcagctca cgagcctgcc gctgccgctg tacacgaacg
    25141 acgagatcaa gctctgcacc gaaggcacct gggcggagat gcaggcgggc tgcaacgtgt
    25201 tccagccggc gctggtctcc aaccccggct ggttcggctc gatctactcc gccgagacgc
    25261 ccgagatcgg cctgcaggcg gccctggtcg gccagcccga gaactgatct tcaggctgcg
    25321 ggagggtgtc tcgccctccc gccttcgggt gggatcccgg ttgatgaccg aggctgcgcc
    25381 ggcgctgcgc gtcgagaagg tcgccaaggc ctacggcgcg accgtggcgc tggacggcgc
    25441 ctcgttcgcc gtccgggcgg gcgaggtgca cgccctcctg ggcgagaacg gggccggcaa
    25501 gtcgaccatg gtcaagctcc tgtccggcct ggtgcagccg gacagcggca gcatcagcct
    25561 ggcgggccag gaggtccgcc tgcgtcggcc ggccgaggcg caccggcacg gcgtgcagac
    25621 cgcgttccag gagatgaccc tggtgcgcga cctgacggtc gcccagaaca tgctgctgcc
    25681 caacgcgccg acccttctcg gccagctgcg cgggcggctg ggcgagcgcc gggtggcgga
    25741 gcatctggcg cgcctcggcc tggagagcat cgacccgcgc gccgagatcc gcgacctcga
    25801 gctgccgcag cggcagaaga tcgagatcgc ccgcgccgtc tttcgccgcc cgcgcctgct
    25861 gctgctcgac gagccgacct cgacgctgtc gggccgcgac atcgactggc tcggcgagct
    25921 gatcgcgggc gtcggccagg atggcgtcac ggtggtgttc atctcgcacc gcctgtcgga
    25981 ggtgcgccag ctctgtgacc gcctgacggt gctgcgcaat ggccgggaca tcggcaccgc
    26041 cgaggtcgac gcgatcggcg acgacgaggt gatacggatg atcatcggcc gctcgctcgc
    26101 cgccaccttc ccgccgcggc cggccccgcg gccctggccg gcggatgccg tgccggtgct
    26161 ggcagcgagc ggcctcgcca ccgacggcaa gctcgccgac gccggcttcg cgctgcggcc
    26221 gggcgagatc ctgggcgtcg ccggcctgca gggcatgggc cagcgggagc tgttcttggc
    26281 ctgcttcggc atgctggcgc tgcgtcgcgg cgagatccgg gtcgacggcc gcaaggtgac
    26341 cctggcctcg cccaaggacg ccgtgcgcgc ccggatcggc atcagcctgg tgcccgagga
    26401 ccgcaagacc gagggcctgt tcctgaggct cgacggcagg cgcaacatct cgctgccggt
    26461 gatcgaccgc ttcacccgct ggggcctgat cgacgaggcc cgggaaaccc gggccgtggc
    26521 cggcgtcctg gccgaggtcg aggtccagca gcgggcgatc tacaccagcg ccggggcctt
    26581 cagcggcggc aaccagcaga agatcgccat cggcaagtgg ctgctcgccg gcagccgcat
    26641 cctcttgatg ttcgatccga cccgcggcgt cgacgtcggc accaagcacc agctctatct
    26701 gatgatgcga cgcttcgccg acgccggcgg ctcgatcctc ttccactcga ccgagatcgc
    26761 cgagctcgtc aatctctgcg accgggtgat ggtgctctac gccggccgcg tcgccctgga
    26821 gctcgagggg gagccgatcg aggagcaggc gatcatgcgc gcggccctgg gcgagctggc
    26881 ccggccggcc ggcatcgcgg catgagcgcc gcggccctgc cgctggcggg ccggcgcccg
    26941 cgccggggca tgcggctcgg ccggcatcgc ggcctcctgg tcgccctcgt cgtgttcgcg
    27001 ctgctcttcg ccgttgccga cgcgatcacg cctgggtcat tcagctactt cgagctcagc
    27061 ttcatgtcct cgggcggcgc caccctggcg ctggccggca tgggccagac ggtggtcgtc
    27121 ctgaccggcg ggttcgacct ctcggccggc gcggtggtgt ccctggtcaa tgtcgtgctt
    27181 gccacctgga tgggccccga gccgccgagc cagatcctct tcggcctcgt cgccatcggc
    27241 atcggcgcca cggtcggcgc ggtcaacggc ttcttcgtcg ccgtggtccg cctgcagccc
    27301 atcgtcgtca ccctctcgac catgttcatc gtccagggga tcacccttct gatcatggac
    27361 aagccgggcg gcctgatcgc gcccgagttc tcggctttcc tcgccggcga cgcgatcccc
    27421 gaccggctgc cggcgccggt cgtggtcatc gccgcagcgg tcgccttctg gctcctgctc
    27481 aagaacacgc gcttcggcgt cgccctctat gcgatcggct cggacccgga ggcggccagg
    27541 gccgcgggcc tgccgactcg ggccatccag ctcggcgcct atgtcgtggc cggcatgttc
    27601 tacggtgcgg cgggggcgtt catctcggcc cagaccgggt cggccgaccc cttgatcggc
    27661 gggccgatgc tgctgcagat cttcaccgcc gtggtgctcg gcggcacgct cctggggggc
    27721 gggcgcggcg gctgcctcgg cacggtggtg ggcgcctaca ccctggtgct ggtggtcaac
    27781 atcctgctcg tcctcaacgt ctccgcctac tacagctcgg tcgccgaggg cgcgatcctg
    27841 atcatggccg tgctcgccgc ctccttgaac cggcgctcgg cgatcgccga ctatctccgc
    27901 ctcggctggc tcgagctccg tgcccgccac gacggcacgg caccgtcagc gcatgccggc
    27961 gaagcacggc ccttcgccct gcagcggccc gaggtggcac tggcgcccgt gtcctggctc
    28021 gagcgccacc gcgagctgct gcgctacgtc ctgccggcct atgccggctt cgtcctggtc
    28081 ctggtggcga cccatctcgt ctacggcaat gccctgacca accccgccta cttcaactcg
    28141 ctgatcgtgc tggcctcgtt cctggtcgtg ctggcgcttg gccagggcgc cgtgatcctc
    28201 accggcggcc tcgacttgtc gctgccctgg atgatcgggc tgtgcggcat cctggtggct
    28261 gggctggtgc gcggctccga cgaggcggcg ctctgggcga taccgctggg cctcgcgctc
    28321 ggggccggcc tcggcgccct caacggcgcc gggatcgtcc tcctcggcct gccgccgatc
    28381 gtcatgacgc tggccatgaa cggcatcctg cagggtgcgg cactggtcta cagcaacggc
    28441 acgccggacg gcttcgcctc gccgggcatg cgctggttca tgaccggccg gctcctgggc
    28501 gtgacacccg tggtctggtt cgtcgccgcc ttcgtcggtg cggcactggt gctgctcggg
    28561 cgcaccgcgt tcggccggcg cgtctacgcg gtcggcaacg gtgccctcgt ggcccggctc
    28621 agcggcgttg gcgtcggcgg cacgctgatc ggcgtctacg cgctgtcggg tctctgcgcg
    28681 gcgctggtcg gcatcctgct cgccagcttc agcggccagg ccagcctcgg catgggcgac
    28741 ggatatctcc tgccgtcgat cgccgtcgtg gtggtcggcg gcacgctgat caccggcggc
    28801 cgcggccact atctgggcat gctcggcggc gtcctcctgc tgaccgcgct cgccacgctg
    28861 ctggccggca cgacgctgcc gttcgccgtg cgcgacatca tctacgggct cgtcgttctg
    28921 gccgcggtcc tggcgctgcg cgagcgtcac gcatgagccg gggggcgggg catcgcgggc
    28981 cgccgccggg tcggattcca gactgaaggg gagagcgagg atgcggatcg aggttctggt
    29041 cgacgtcaag accacgctgg gcgagggacc cttgtgggac gtggacgagc agcggctgta
    29101 ctggatcgac agcttcgacg gccgggtgtt ccgctgcacc gccgatgggc gcgaggtgcg
    29161 ggcctgggac gtgccgatga agatcggctc gatgtgcctg cgcaagggcg gcggggccgt
    29221 ggtgtccctg cagcgcggct ttcatttcct cgacctcgca tccggcgagg tcaccttgat
    29281 ccacgacccc gagccggaca agccgcacaa ccgcctcaac gacggcaagg tcgaccggcg
    29341 gggccggttc gtcgcgggct cgatggacac gcaggaggcc ggccccaacg gcagcctcta
    29401 ccggctcgat cccgatctga gcgtgacccg gctcgaggac ggcatcatcg tcagcaacgg
    29461 cccgtgctgg agccccgacg gcggcacgtt ctatttcacc gattcctggt ccggcgaaat
    29521 ctcggcctac gactacgatc tcgccaccgg cgccatcgcc aacaagcgca ccttcgcccg
    29581 gatggaatat cagggcgccg gcttcgacgg tgcgacggtc gatgccgagg gcggggtctg
    29641 gagcgctgtg gtctatgtcg gcaagctcgt ccgctacctg cccgacgggc gggtcgaccg
    29701 ggtgatcgac atgccggtca agaaggccac cagcgtcaat ttcggcggcc cgaacctgga
    29761 catcctctac gtcacctcga tggccaagcc gccgcttccc cgcttccccg acgacccgca
    29821 gccgcgcggc tccctgttcg ccatccatgg tctcggcatc cagggtgttc ccgagccgcg
    29881 cttcgccggc taggtgccgg tccgagcggc agggcgtgac gaaccaccga aaggcgctac
    29941 gaggtcagcg cccccgccgt catgccctgc accatgaact tctgcaggag aatgaaggcc
    30001 acgaccaagg gcaggctggc cagcatcgag aaggccatca tgccgctcca gtccgtgacg
    30061 aactcggcca tgaggctgta gatcgccatg gtcagcaccc ggttctccca ggcctcgacc
    30121 agcaccatgg ttcaagagat ggcactcatg cgcgtgcagc cgtcgaccat cgccgccgcc
    30181 tccatggccg gcgatatgtt gatgaagtag ctgcctggca tgtggatgta gaacggtaga
    30241 gtaaatgccc aaagccaaag acgaaagccg ccgccggtat ccgtccgaac ccttggcccg
    30301 cgctgaagtt ttgcagcgcc tcgcaccaca tttcgaattt ttcgaagagg ttaaagcaga
    30361 aagcgctaat ggggacgtca tgcggattga cgcggtctcg caatgccggt tgaccggctg
    30421 gaccttcggc tgggagttta agcggtcgca cctgtttaaa agtgagttcg ctgacgccat
    30481 gcggcaggga atccactatc gactgtctcg tatcgccgac gcccgcttgc ctacccacaa
    30541 ggggttacaa ttgccagcaa tagttctttt ccccgattgg cttggcgaac acgacgatga
    30601 cgtgacgaac tacggtaggg aagccgaagg aatgcgtttg gtgggcgctc aatttcgtgt
    30661 cggaacgatg cgacaatcgg gcgatgaaag gttcgccttc attatgggcc aatctgccat
    30721 ttggcattca gcgtcgggtt ggaccaagaa tgcggaaggc ctgttgttgg gcaaacgcag
    30781 ccttggctcg acgcgaaaga acgaccgctg aaataggcgc ttggccccta cgacgtcagc
    30841 gccccggccg tcatgccctg caccatgaac ttctgcagga ggatgaaggc caccaccaag
    30901 ggcaggctgg ccagcatcga gaaggccatc atgccgctcc agtccgtgac gaactcggcc
    30961 atgaggctgt agatcgccat ggtcagcacc cggttctccc aggcctcgac cagcaccatg
    31021 gcgaagagga actcgttcca ggcgagcagg aaggcataaa ggccggcacc gatgaaggcg
    31081 ggataggaga ccggcaccag gatcctgaag atcgcaccca tgcgcgtgca gccgtcgacc
    31141 atcgccgcct cctccatggc cggcgagatg ttcatgaaat agctgcgcag catgaggatg
    31201 cagaacggca gggtgaaggt caggtagacg atgatcagcg aggtccgcgt gtcgtagagg
    31261 ccgagcatga cgaagacgcg gaaatagggg atgcagagca ggatcagcgg gaacatctgc
    31321 gtgatgatga ggaacatcag gacgacccgc tggcccatga agttgaaccg ggcgagcgcg
    31381 taggcggcaa gtgcgcccag gaacagcgac aggaacgtca cgaagagcga gatgaagatc
    31441 gtgttgagga agacgacgat atagcgcggg ttggtcagga tcttgccgta gctggcgagc
    31501 gtgaagtgcg ccgggatcca gctcggcggg atcttcatca cctcgagatt ggggcgaacc
    31561 gagaccgtca gcatccacag gatcggcagc gagacgatga cgatggcgag gatcaaaagg
    31621 ccataggaca tggcattcag cagcaaccgc cgctggtcca tgccaaggcc gcccgccagg
    31681 cttcgctgtc gcatggccat ctcccccgtc aacctgcggt gctcatggat cgccgcatgt
    31741 agaaggcgct gacgacgaag aggacgacga acatgacgac cgcctgggcc gaggcgtagc
    31801 cgaagtcgag cttctggaag ccg
//
LOCUS       DAOTFI010000015        31721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1602_length_31721_cov_6.722068, whole genome shotgun sequence.
ACCESSION   DAOTFI010000015 DAOTFI010000000
VERSION     DAOTFI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 31721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31721
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_31721_cov_6.722068"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="P5558_02535"
     CDS             <1..737
                     /locus_tag="P5558_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRY23219.1"
                     /translation="AGQLRPLDDGPVDVVLAPAAERSAGLLVALLPRAWIDLNRSPAE
                     LDPSDIEGLVPWPTEITAKVRAGLGLVPSRIGGHRLLRRPLRADEIAQRLATVHRPFH
                     QELARQLTCLQARHGVAVLLDCHSMPRASVTASGPPVEIVVGDHHGASASRAVTELAL
                     SVLRRHGFRVATNRPFAGGHITGTCGRPEQGLHALQIELRRDLFLDERDYRLHGGMAA
                     LGAALADVVTTLGELALRLPGRSAAA"
     gene            745..1971
                     /locus_tag="P5558_02540"
     CDS             745..1971
                     /locus_tag="P5558_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HRY23220.1"
                     /translation="MAGEPDVAVIGGGPAGLMAAEILGGEGLAVALYDRMPTVGRKLL
                     MAGRGGLNLTHGEPLARMLDRYGAARETLRPMLEAFDNAAVRAWAEGLGQPVFTGSSG
                     RVFPRALKASPLLRAWLARLAERGVAIHTRHDWQGFGADGSLRFRLADGSEVLARPKA
                     TVLALGGASWPRLGADGGWVEPLRAVGIDVRDLVPANPGIDIAWSPGFVERFAGRPLK
                     TVEVELDGDRQRGDLVLTAYGLEGGAIYTLGPAIRDVLARDGKATLNLDLRPDGDTES
                     IAARLAKAFGKGRSRANALRALGLAPQAVAILREVPGGLPAEPLALAALLKAVPLEVT
                     AQRSLARAISTAGGVAFDGLDKRLMLRDRPGVFVAGEMLDWEAPTGGYLLQACLATGH
                     WAARGVSAFLAAQDNA"
     gene            2034..2303
                     /locus_tag="P5558_02545"
     CDS             2034..2303
                     /locus_tag="P5558_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23221.1"
                     /translation="MASKRALIGIDVGTSGTKVLVVDEAGAILARHTESYPLDTPRAI
                     PSARHQSAGPGSGPWWQSSVPASIMSESASPARCTGWWRSTRPTG"
     gene            2207..3535
                     /gene="xylB"
                     /locus_tag="P5558_02550"
     CDS             2207..3535
                     /gene="xylB"
                     /locus_tag="P5558_02550"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRY23222.1"
                     /translation="MVAELGAGVDHVGIGLSGQMHGLVALDEADRVIRPAILWNDQRT
                     GRQCAWITERAGGLDGLLALTNNPMLTGYTGGKVVWLREEETGNYARLKRILNPKDHI
                     RLMLTGEHATDVSEASGTGLFDVARRDWSWELVDRLDLPRSILPKAYESHEITGRLRD
                     EIAREFGLPRGLPVMGGGGDAVIQTTGSGLIDPGTAQTIIGTAGIAACALDACAPNPG
                     GRLQVFCNNDPARWHCMGVSMNAGGAFAWLRQTLGAFATDELEFDAMTARAEKVPAGA
                     EGLFFLPYLIGERCPHPDPDARGAFVGLTLRHGAGHLVRAAMEGVVFSIREMLELMRP
                     LGLEATTLRTSGGGTSSPLWRQIQADATGCEVQTVSGAAEGGAYGAALVAGVGAGIWP
                     DLSTAIKGIAVETRTTPDAANRARYDAAFAVYRELYPTLKPRFDAIAALP"
     gene            3566..4195
                     /locus_tag="P5558_02555"
     CDS             3566..4195
                     /locus_tag="P5558_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRY23223.1"
                     /translation="MSIEMWLAFALASAVLLAVPGPTIMLVVAYALGQGKRAAAATVA
                     GVALGDFTAMTASLLGLGVLLATSATLFTALKLVGAAYLVYLGVRLWRAPVMAADSGI
                     ATPVVSGRRMLAHAWLVTALNPKSIVFFIAFVPQFLDSSRPLFGQMVVMEVTFVLLAT
                     LNAAAYAGLATSARRLIRQPTAQRLVNRVGGSFLIAAGVATVAWRRAAS"
     gene            complement(4196..4951)
                     /locus_tag="P5558_02560"
     CDS             complement(4196..4951)
                     /locus_tag="P5558_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23224.1"
                     /translation="MSLALFDLAGRRALITGSGQGIGNAIARGLAAAGASVVLNGRDG
                     AKLEAARADIPGATAHVFDVTDQPAVTAAIDRIERDEGPIDILVNNAGIQRRAPLQDM
                     PAETWHEVMKTNIDSVFYVGQAVARKMIPRGQGKIINICSLTSEAGRQTIAPYTTAKG
                     AVRMLTKGMCADWARFGMQINGIGPGYFRTPLNKALIEDPKFNAWVEQRTPAGRWGEL
                     EELQGAAIFLASDASRFVNGHILYVDGGLLAVI"
     gene            5038..6864
                     /gene="ftsH"
                     /locus_tag="P5558_02565"
     CDS             5038..6864
                     /gene="ftsH"
                     /locus_tag="P5558_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRY23225.1"
                     /translation="MDRKQQINLGYALLAFLLLMMFQGWWAASRQIETIPYSEFQQLL
                     DKGAIDEIRVSTDYIEGKLRTPTESGAQYFRTTRVELDMADRLAGHDVRFAGVIESTF
                     LRDLLSWVLPILLLIGFWMFMIRRFAEKQGFGGLMQVGKSKAKVYVEKDTKVSFADVA
                     GVDEAKEELQEVVSFLKDPQTYGRLGARIPKGILLVGPPGTGKTLLARAVAGEAGVPF
                     FSISGSEFVEMFVGVGAARVRDLFEQAGKAKPCIIFIDELDALGRARMSGQFGGGGHD
                     EKEQTLNQLLAELDGFDPSEGIVLLGATNRPEILDPALLRAGRFDRQVLVDRPDKTGR
                     LQILEVHIKKVKKLGADVSLEQVAALTPGFTGADLANLVNEAALLATRRRAEQVTMED
                     FNNAIERIVAGLEKRSRLLHPHERKVVAYHEMGHALVAATLPTTDPVHKVSIIPRGIG
                     ALGYTIQRPEEERFLMTTKELEEKMAVLMGGRAAEHVVFGEISTGAADDLDKISDIAR
                     HMVTRYGMHDATAQVVYDPQRQSFLGENGMPVARPRNYSEATAREIDLAIRDLVEGAF
                     ARAVDIVTQRRADLDRGAALLLERETLTSAEFPLLRRQKLAA"
     gene            6883..8949
                     /locus_tag="P5558_02570"
     CDS             6883..8949
                     /locus_tag="P5558_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRY23226.1"
                     /translation="MRKFLVTVLLILVGLVVVAVALPFFLPREMIKTQLEGRVSAALG
                     RELVVEGPLTFRPWRPFAFSLADVRLANPAWAENPDLARVALVDVEIDALAWLGGTID
                     VVRVVIDQPVLALEVDAEGMPSWRFEGPDESDDSSGDGDGEASDLPDVRIGDVALTDG
                     AVTYIDHGTGERREFSGIGLGARTEAESGALLLDGAATSAGQQATLEGRIGDLAALLD
                     GEPSSVNLELAAPGLGLVAGGEASPAGTAVLAVTVDMAPRTLLDWLGQPVALPEGRLE
                     SATFTVDLESAPDSLGLHALSLAVDELLVQGDLDLAFGERPRLAGRLDLGDLDLRPYL
                     PAETPTDGAAAAAADAPAAEADGWSTEPLDLPLPLPLDLDLELVMNSLREARLETGPG
                     RFLAQADAERASLEIAELALYGGGATGTAALTSDDPLKVEASLDATGVELLPVLQGAA
                     DIDYLEGTGNVRFTIEGLGDSVATLVESLAGDGEVLVRDGAILGINIAATIRQVMTLG
                     VESAATQPQRTDFAEAGGTFTIERGIVRNDDFNLRAPVLRIEGAGEVALPPRTLDYRL
                     TPHVASTLQGQDAGGEPDLRLGVPLVIEGAWASPAVRLDLGGALSGDITDPSELAETV
                     ARLAADPANLEALKEMFGGDLPGSLGDALEQLPGVLGGSSGNDDSEAGAPAKRLRDAL
                     GGLLGR"
     gene            8978..10018
                     /locus_tag="P5558_02575"
     CDS             8978..10018
                     /locus_tag="P5558_02575"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRY23227.1"
                     /translation="MIDLRSDTLTRPTPAMRRAMAEAEVGDDVYGEDPTVRRLEERTA
                     GLLGKEAALFVPSGTMGNQLALRCQTEPGDEVLLHEAGHIALYERGGYALLSGVVLRL
                     LPGEGGVVTPATVEGAIRPAAIAAYGIYPPARLLALENTHNAGGGTVWPPELLAATAA
                     AGRAAGLALHLDGARLWNAAIASGASPARLAAPFDTVSVCFSKGLGAPVGSALAGRTD
                     LITKARRFRGLFGGGMRQAGVLAAAALHALDHQHERLADDHAAARRLAFGLAELGPFR
                     LDPAAVRSNIVIAEVVGRPAAGIVAAARERGLLCLEVGPGRLRFVTHLDLTGDAVDRA
                     LAILADVVREVS"
     gene            10291..10983
                     /locus_tag="P5558_02580"
     CDS             10291..10983
                     /locus_tag="P5558_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRY23228.1"
                     /translation="MLGASDTRINVRSAPRVASGNIVASTKGGELLRVLEAAEEGQYT
                     WYRVTTLPGSAESFEGWIRGDLLASAALPEPTLPQVLATPVPAADVPDAARRASEAAA
                     PVPLSQRTDWSRDLLRLFPAIEGCAAVGSAPPITVLRAIGRSRGLAEIIMSDAAGRRW
                     DCVIRETGGTPMRYDPLSGAVYMRDRMGNEPFFAIGEERPALDPNCFRFERVIDPTNG
                     EMLGWLYYRTCP"
     gene            10980..12419
                     /locus_tag="P5558_02585"
     CDS             10980..12419
                     /locus_tag="P5558_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRY23229.1"
                     /translation="MSGLVELGATAAVAELRAGRVTPLELVEAAIERIERLDPLVNAV
                     PIRCFDRARAHARSLMQGTAPAEPGRGWLGGLPIVVKDLFEVEGVRTTYGSPIFAEHV
                     SEVTDLHVGQLEANGAIVLGKSNTPEFGAGANTFNEVFGATRNPWNTALTCGGSSGGS
                     AVALATGMAWLADGSDLGGSLRTPAAFCGVVGLRPSIGRIMRGPASRRFQTLTVNGPM
                     ARDVADLALMLDAMSGTSLDDPLAAEAPAMSFAAAARMPPRLQRVGFSADLGGFVPVD
                     PEIARLAGQAAARMAELGAAVIENRSPDFGPAREVFEVLRAAHFAAEKAPLLEAERDK
                     LKPEVIWNIERGLALTADEIGWAERERSLIQARVAAYFREHDLLLCPAAIVPPFPVEL
                     RYLEALGEHRFPTYIDWVAVTYAITLSGCPALSLPCGFTEAGLPVGLQLVGPPQGEAR
                     LLAAARALEEVLAVGSARPIEPRSPLAGA"
     gene            12495..13115
                     /locus_tag="P5558_02590"
     CDS             12495..13115
                     /locus_tag="P5558_02590"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRY23230.1"
                     /translation="MLRIWGRTNSINVQKVMWAVGELDLAHERLDAGGAFGGLDGDAY
                     GRLNPNRRIPVLEDGETVVWESNAIVRYLAARYGVGTLWAEDPALRSVADRWMDWQVT
                     TLLPPLHPIFWGLVRTPGPERDMAAIDAAAKAIQPLWQILDEHLSKSRFIAGDAFSMG
                     DIPLGCAFWRYANLDVERPSLPNVQLWYAELKKREAYRRHVMLPVT"
     gene            13112..13939
                     /locus_tag="P5558_02595"
     CDS             13112..13939
                     /locus_tag="P5558_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRY23231.1"
                     /translation="MTAAGDVAEDSPPPEAPPPSLRAGLRDLYYGADRRAARFQYGLL
                     LFDVATILFFLVVTFVADAPWLIYADLVLAVVLSLDFAARLYIAPRPGTFLAHWVTIA
                     DLLVILSLIVPLLTENYTTLAFLRVLRAVRLARSYHLFGMLRRRHKWVQEHEDALQSA
                     IHMVLFLFVTTAIVYVQQFGRNPAIGSYVDALYYTVATLTTTGFGDITLVGEGGKLLS
                     VLIMIVGIALFVRLVQTIFRPAKVKHRCPECGLIRHDPDAVHCKHCGEVLDIPNEGD"
     gene            complement(13946..14431)
                     /gene="gpt"
                     /locus_tag="P5558_02600"
     CDS             complement(13946..14431)
                     /gene="gpt"
                     /locus_tag="P5558_02600"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRY23232.1"
                     /translation="MNDPYQKFFPVSWDQLHRDAKALSWRLASGGPWKGIVAITRGGL
                     VPAHIISRELDIRVIDTICIASYDDQTQREPKVLKSVDGDGEGWLIIDDLVDTGQTGR
                     IVRERLPKAHFATVYAKPAGREIVDTFITEVSQDTWILFPWDTEPQYVKPISANHQRP
                     D"
     gene            14548..14760
                     /locus_tag="P5558_02605"
     CDS             14548..14760
                     /locus_tag="P5558_02605"
                     /inference="COORDINATES: protein motif:HMM:NF018413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HRY23233.1"
                     /translation="MDEELPRKKPSEFALGSELDRHSLDELEALAAMLRAELERVGRA
                     IAAKRDVRAAAEAFFKRQEPPAPADG"
     gene            15031..15591
                     /locus_tag="P5558_02610"
     CDS             15031..15591
                     /locus_tag="P5558_02610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRY23234.1"
                     /translation="MFSIRLEQPADSVAIEELLDLAFGADRRKKTSYRYRDGIAPLAD
                     LSFVAEAEAGLLVGSVRYWPILAGSTPALLLGPLAIEPARQGRGVGRALVFHSLDVAA
                     QAGHRLVFLVGDPAYYQRFGFDPVPPGIVMPNESPARLQYRLLDPSLALPEKATLRRS
                     QSRAAHRAGRGASLAGKAIAGASCIP"
     gene            15604..15680
                     /locus_tag="P5558_02615"
     tRNA            15604..15680
                     /locus_tag="P5558_02615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15638..15640,aa:Pro,seq:tgg)
     gene            15765..16970
                     /locus_tag="P5558_02620"
     CDS             15765..16970
                     /locus_tag="P5558_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRY23235.1"
                     /translation="MAQPPLQLVIGNYNTSSWSLRAWLAMRLARLAFTEIRVPLRRED
                     TPASIRRYSPSGKLPVLLVDGTPVWDSLSIAELVAELDPAVWPADAARRAEARSISAE
                     MHSGFADLRRLMPMDVVSRFAPPGRMPRGLTADIARISAIWRSCRDRYAADGPFLFGA
                     FSLADAMMAPVASRLVTHAVPLDTMLDDYVDSVMSWPDMVVWRDAADAEVEALRDRGA
                     LEIQSVARAGREVPDEPLGGAQLLAATAVEPSPPRRVEPQVVVSLDATPDATSDEAPE
                     AVFEEVPAIPVEPSAMPEAAPAPQGRAEPPRQDASEQMETPAIAASPSPAEQQSPPAD
                     EPPPPAPPPARTAPAPSAASAPRPPVVEPATRPLPEATPEAGAQVEAPRRPGGTTIKP
                     IGSGILRRR"
     gene            complement(16933..17418)
                     /locus_tag="P5558_02625"
     CDS             complement(16933..17418)
                     /locus_tag="P5558_02625"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRY23236.1"
                     /translation="MSCRVSKGPLPGYRRCVGIMLLDRANRVFVGQRHDTRAEAWQMP
                     QGGIDKGENALDAARRELQEEVGTNRADLLKESALWRSYDLPPEIARRLWRGRYLGQT
                     QKWLAFRYLGEDADIMLDGEHPEFCAWRWLAPDELVERIVPFKRDVYLSVVEEFRCLW
                     A"
     gene            complement(17450..18523)
                     /locus_tag="P5558_02630"
     CDS             complement(17450..18523)
                     /locus_tag="P5558_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRY23237.1"
                     /translation="MPARLLPPAFLPAVDWRSPRLPLVVGAAMLGGSLLVASASLLTG
                     HEAPYRHVGLPSVQGPLAEARRPRPPALFGLASPAVLPPLSSLPSPDPEAPRLEVDPD
                     LVETIDGLTLPRIAADGRQARMLYARGADPTAVPAASPIEIAILVTDLGLDAERLTQS
                     VALPPAIGLAHTPYAAHLPSWQRHARWHGHEVLLELPLQAADHPTSDFGPWALDPSKP
                     PPELIAGTRRVASRGEAYLGLVAASGAFAAAPERFTPVAAELAARGLALVELGEPLLA
                     ATAAANGVAYARASGPLDAVPDAAAIDAALARLEATARRDGRALAYAQPYPLTFDRLW
                     HWAQGLEAKGIRLVPVSRFLAEP"
     gene            complement(18614..19864)
                     /locus_tag="P5558_02635"
     CDS             complement(18614..19864)
                     /locus_tag="P5558_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY23238.1"
                     /translation="MITGCMMPPPSAQAQSGSGDTIRQLKLFGDVFERVRKEYVEEVT
                     DQELVESAINGMLTSLDPHSGYLDAGRYRDMQVQTRGEFGGLGIEVTMENGLVKVVSP
                     IDDTPAFDAGIQAGDLITHIDGEPVLGLSLSEAVERMRGPVNSEIELKIVRENREEPL
                     SVNLARAIIKISPVRARAEDDIGYVRLTTFNEQTNDIMREEVAKLRDQLGDRFRGLVL
                     DLRNNPGGLLDQAVAVADAYLDQGEIVSTRGRRSESIQRFNARSGDIIDGLPMVVLIN
                     GGSASASEIVAGALQDHRRSIIMGTQSFGKGSVQTIMPISGEGAIRLTTARYYTPSGT
                     SIQAKGIAPDIEVHQATIELQDDTSGRREADLRGRLDGEQPGDQAASGEAEPTLDSTD
                     YQLARAIDVLRGIALYEARATQRG"
     gene            complement(19909..20859)
                     /locus_tag="P5558_02640"
     CDS             complement(19909..20859)
                     /locus_tag="P5558_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY23239.1"
                     /translation="MLALQRRLEAVLAELMQLDRAEVADRRRIAQLRAIAASYVEPFA
                     EAKQALAASRAARLAFANEELELRAAGLRVRSRQLALERRSTGLEAAERAIRLDLAEA
                     TRRELAARRDAQRALARHERVLESRLLASLPDAAARLPSPAHLGPTASRLKPSVLTAR
                     AVLDRQPDRVAVLFGEPAASAVAALSGMGQVMPVAGSVVGHFGQGPKALLDQGITIAV
                     EERRLVRAPRDGRVAFAGPFKGFGLLLIIEHGDEYHSLLSGLSRLAVSVGAAVRAGQM
                     VGTLEPTQGGTAQLYVELRRRGVPVNPLPWLAAGQDKVRG"
     gene            complement(21200..22747)
                     /gene="gpmI"
                     /locus_tag="P5558_02645"
     CDS             complement(21200..22747)
                     /gene="gpmI"
                     /locus_tag="P5558_02645"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRY23240.1"
                     /translation="MRRPVVLCILDGWGYRRESADNAVALAETPVFDKWFAAAPHCLL
                     TTHGKAVGLPEGQFGNSEVGHMNLGGGRVMLQELPRIDAAIEDGSLWQATPFVELVQS
                     ARGGSGCLHLVGLVSPGGVHAHERHLFAIARAAAEAGLRLRVHAILDGRDTPPRSAAA
                     SIEKLESVLGPLPDARIATVCGRYHAMDRDRRWERTRVAYEAMVAGRDTAAEGVMRAA
                     SAIDAIRASYARDVGDEFVPATVISDYAGMADGDALLCFNFRADRVRQLLRAFCLEDF
                     DGFARVRRPKLSVLAGMTHYAEDLAGCMLVLFPPQPADDLMGEVVARAGLKQLRMAES
                     EKYPHVTFFFNGGEERVYPGEERILVPSPKVATYDLQPEMSAPELADRCVAAIGEERF
                     DFILVNFANPDMVGHTGVLGAAIRAVATVDRCLGRIIEAVLGVGGAILVTADHGNCEL
                     MRDPATGGPHTAHTTNPVPCFLVGAASGTSLRDGRLGDVAPTLLALLDIPKPAAMTGH
                     SLIARRA"
     gene            complement(22911..24854)
                     /locus_tag="P5558_02650"
     CDS             complement(22911..24854)
                     /locus_tag="P5558_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRY23241.1"
                     /translation="MSRSDFCPVAQPSGKKITPHAASPEVAGIALTLNILNDYTRCTC
                     IKPRLSCDNRLRPADGHMQTRTGSIGLRPWTLALMATVAVVAGSGMASANTIAESVQL
                     ALETNPEVGIVQNNRKAVDQELRQARAGYLPSIDFRGAAGPEWSDNFTTRSFEGGSET
                     KLRSEADLSLTQMLFDGFATQSEVDRQRARVDSAAYRVEEAAEFVGVNAIEAHLNVLR
                     NQEIVRLNEANIVQHERILGQVRDLEESGRGDIADVRQTEARLARAFDTLAIARGSLA
                     DAIAVYQRVVGVRPGSLEDSEAPVAALPPSPEDAANLASVSSPTVRIAAADVDVAAAE
                     TRGSRAGFYPRFDVEVGAGLNHDIDGAEGRDNDARALLVMRYNLYRGGADMALEREAF
                     HRMNEARVALENTRREAEQEARISYNALDTARDRIEASVAKVEAQRRTRDAYASQFEI
                     GQRQLLDLLDAENELFIDRVSLITATYTERFAVYRVLAVVGELLDTLEIARPREVISI
                     NRKPADEQTPEAIEQKSAPILDPRSEPRPVRGKERGEPPFDALDMAPLIDGSDQPVEA
                     AAAAALEPATQLAAASGPSVAGIVAELAGGQPVDVTSTAAGPESQPTVRDSSAPATYA
                     SFGAFVESVFSGRRDGGEPRTAN"
     gene            24946..27195
                     /locus_tag="P5558_02655"
     CDS             24946..27195
                     /locus_tag="P5558_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRY23242.1"
                     /translation="MTDGAMLLRERDTREVDGGGRAEGAGSGDRPPVEAASWQIRESF
                     ATRDDSLLRALETLTQLHERPTSGQALGAGLPLPPDGLMTPALAPRALERAGLSARLV
                     RRAISAIDALSMPCILFLKDRRCVVLVERRGAVLRVVVPETGRGTVEVELADLERRYD
                     GHALFARPEIGYEQRDRPIAEERKGHWFWSTLLRQWPTYAEVMIAAVMINAFALASPL
                     FVMNVYDRVVPNNALDTLWVLALGAFIVFAFDFLLKSLRGYFIDTAGRIADIRLASRI
                     FEQVLGIRMAARPASAGAFANNLREFESLRDFFTSATVTTLVDLPFVLFFITIIWLIG
                     GPVALVPAIAVPVVIGVGLVMQIPLNRVVRRTFKESAAKHGVLVEAINGLETIKSIGA
                     ESRLQRGWERYVAATAESANRSRFLSAITVNVAGLAANLVTVGVVVVGVYRIGEGLMT
                     VGALVACTIIAGRAMAPLGQVAGLLTRYHQARMSYDTLNRVMAMPIERSPDMRFLDRP
                     RLSGAIEFKNVTLTYPGQKLPALSDVGFRLEAGERVGLIGRIGSGKTTIEKLVLGLYS
                     PEKGAVLVDGTDLRQLDPADLRRNIGCVPQDVFLFQGSVRENITMGASFADDAAVLRA
                     AEISGVEDFVSRHPLGYDMPVGERGELLSGGQRQAIALARALLLDPPILILDEPTSAM
                     DNGAESRFKTRLGEELAGRTLLLVTHRASLLGLVDRLIVLDGGRLVADGPRDQVLKAL
                     AAGQIKGAN"
     gene            27197..28549
                     /locus_tag="P5558_02660"
     CDS             27197..28549
                     /locus_tag="P5558_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRY23243.1"
                     /translation="MRSATAIDTRALRTDPIAAAAAAGGYTVEDLRPKRLSHVLLIAI
                     VAFLAVFLAWASRATLEEVTRGEGRVISSQQTQVVQNLEGGIVAALLANEGDIVEAGQ
                     VLMRIDNIRAASDFREHLARSYALRAGLARLEAEVAETALEFPPDILAEARDIANAET
                     RLFQSRQEELQSELEILRRQAEQREQELAELQTRSRQYEESLRLAREELRILTPLAER
                     RVVSQSELLQLQRQVNDLDGQLGQTKLAIPRIEAAVREAHQRIENAFSTFRSEAYREM
                     SALQSELAGIREVITAGEDRVRRTEVRSPVLGTVKQLHFNTIGGVIQPGDAIVEITPL
                     EDSLLVEAQVRPADIAFLRPDLPAMVKITAYDFSIYGGLKGHVEQISADTIVDDRGER
                     FYRVRVRTEENSLGTAAEPLRIIPGMTAQVDVITGEKTVLDYLLKPILKARDHALRER
                     "
     gene            complement(29301..30698)
                     /locus_tag="P5558_02665"
     CDS             complement(29301..30698)
                     /locus_tag="P5558_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23244.1"
                     /translation="MTALATATPAPALRWRSRYAALRRIARNGEEELRTALGALARGL
                     RLEAAAAPTPLFVRREVAFRSYPTLHPGACATVVTPPDGAYLHTFFDVPPISPSGRYL
                     AVTRLPFQHRGPYPGDTAEVCVIDLAERTIETVYHTRGWALQLGANLHWHPGRDDLLY
                     CNDRIGGRGLGVVIDLSERRARPLGGPVYALCPRGRHAFGPALDLINATQAGYGVPDP
                     LLGRRRPTTGFATDEGIWRTDLESGRCELLVSVDALLADHPERALLASGRHSLFHTKV
                     NGTGDRLFQVVRSTGLADAPEKVRAMILTLSAEGDAVRLALPHRLWDRGGHHPSWTPD
                     DETILMNLVPPGGHEMRFVRFGHDGNGLAELVGGFRGSGHPSLDPTGRLLLTDAYLNE
                     GYGDANGRAPVRLVDLAAGKESQLMLVDSGPPGLKARRVDPHPVWDRKRRRIVLNVML
                     EQKRQVVLVDLGGSD"
     gene            complement(30902..>31721)
                     /locus_tag="P5558_02670"
     CDS             complement(30902..>31721)
                     /locus_tag="P5558_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRY23245.1"
                     /translation="DDYLQLDASINRGNSGGPTFDVNRTVVGINTAIYSPNGGNVGIG
                     FAIPANLAQPIIAELKEQGFVSRGWLGVQIQPVTPEIAQSLGLDKAEGALVAQVTPDS
                     PAATAGLQVGDVVTAFDGTPIVAVRDLSRLVAAAEIDVPQTVDVWRHGAHEELQAELR
                     TAPTQDVAEAAVETAPDRVLGLDLAALNDETRQAAGLDADAKGVLVVGVTEDADGTVQ
                     PGDVILSVDNRPVTTPAEVAERVRAASEEEHEAVLVLIARQGMQHFATLDVRRA"
BASE COUNT         4472 a        10958 c        11356 g         4935 t
ORIGIN      
        1 cggccgggca gttgcggccg ctggacgacg gcccggtcga cgtcgtcctg gcaccggccg
       61 ccgagcgatc cgccggcctg ctggtggcac tcctgcccag agcctggatc gacctcaacc
      121 gttcgccggc cgagctcgat ccgagcgata tcgagggcct ggtgccgtgg ccgaccgaga
      181 tcaccgcgaa ggtgcgggcg gggctcggcc tcgtgcccag ccggatcggc gggcaccggc
      241 tcctgcggcg cccgttgcgg gccgacgaga tcgcccaacg gctcgccacg gtgcaccggc
      301 ccttccacca ggagctggcc cgccagctca cgtgcctgca ggcccggcat ggcgtggccg
      361 tgctgctcga ttgccactcg atgccgcgcg cctcggtcac cgcctccggc ccgccggtcg
      421 agatcgtggt gggcgatcac cacggcgcct cggcgtcccg cgccgtgacc gagctggccc
      481 tctcggtcct gcgtcgccac ggttttcgcg tggcgaccaa ccgccccttc gctggcggcc
      541 acatcaccgg gacctgcggc cggcccgagc agggcctgca tgcgctgcag atcgagctgc
      601 ggcgcgacct ttttctcgat gagcgcgact accgcctgca cggtggcatg gcggccctgg
      661 gtgctgcgct cgccgatgtg gtgaccacgc tcggcgagct cgccctccgg ctgcccgggc
      721 gctcggccgc tgcctgatcg cgccatggcg ggagaacccg atgtcgcggt gatcggtggc
      781 ggcccggcgg gcctcatggc ggccgagatc ctgggcggcg agggtcttgc cgtcgccctc
      841 tacgaccgca tgccgacggt cggccgcaag ctcctgatgg ccggaagggg cgggctcaac
      901 ctcacccacg gcgagccttt ggcccggatg ctcgaccgct acggcgctgc ccgcgagacc
      961 ttgcggccga tgctcgaggc cttcgacaac gcggcggtgc gggcctgggc cgaagggctc
     1021 ggccagccgg tcttcacggg ctcctcgggc cgggtcttcc ccagggcgct gaaggcctcg
     1081 ccgctgctcc gcgcctggct ggcgcggctg gccgagcggg gcgtcgccat ccacacccgc
     1141 cacgactggc agggcttcgg cgccgacggg tcgctgcggt ttcgcctggc cgatggcagc
     1201 gaggtgctgg ctcgcccgaa ggctaccgtt ctcgccctgg gcggggcgag ctggccgcgg
     1261 ctcggcgccg atggcggctg ggtcgagccg ttgcgcgccg tgggcatcga cgtgcgtgac
     1321 ctcgtccccg ccaatcccgg catcgacatc gcctggtccc ccggcttcgt cgagcgcttc
     1381 gccggccggc cgctcaagac cgtcgaggtc gagctcgacg gcgaccggca acgtggcgac
     1441 ctcgtgctca ccgcctatgg gcttgaaggc ggggcgatct acacgctcgg gcccgccatc
     1501 cgggacgtgc tcgcgcggga cggcaaggcg accctcaacc tcgatctccg ccccgacggc
     1561 gataccgaaa gcatcgcagc ccggctggcc aaagctttcg ggaaaggccg ctcgcgcgcc
     1621 aatgcactcc gtgcgctcgg cctcgccccc caggccgtgg ccatcttgcg cgaggtgccg
     1681 ggcgggctgc cggccgagcc cctggccctc gccgcgttgc taaaggcggt gccgctcgag
     1741 gtcacggcgc aacggtccct ggcgcgggcc atctcgacgg cgggcggggt ggcgttcgac
     1801 gggctcgaca agcggctgat gctgcgggac cggcctggtg tcttcgtcgc cggcgagatg
     1861 ctcgactggg aggcgccgac cggcggctac ctgctccagg cctgcctcgc cacgggccac
     1921 tgggcggcgc ggggcgtttc ggcctttttg gcagcccagg acaacgcctg agccgttgcc
     1981 gaggtgctgc gagcccccta atgctggcgt ggcgagaacg ggggaggcga ggaatggcga
     2041 gcaagcgggc gttgatcggg atcgatgtcg gcacctcggg caccaaggtg ctggtggtcg
     2101 acgaggccgg cgccattctc gcccggcaca ccgagagcta tcccctcgac acaccgagag
     2161 ctatcccctc ggcgcgccac cagtcggccg ggccgggctc ggggccgtgg tggcagagct
     2221 cggtgccggc gtcgatcatg tcggaatcgg cctctccggc cagatgcacg ggctggtggc
     2281 gctcgacgag gccgaccggg tgatccgccc cgccatcctc tggaacgacc agcgcaccgg
     2341 ccggcaatgt gcctggatca ccgagcgggc aggcgggctg gacgggctcc tcgcccttac
     2401 caacaacccc atgctgaccg ggtacaccgg cggcaaggtg gtctggctgc gcgaggagga
     2461 gaccgggaac tatgccaggc tgaagcggat cctcaacccc aaggaccata tccgcctgat
     2521 gctcacgggc gagcacgcga ccgatgtctc cgaggcatcc gggacggggc tcttcgacgt
     2581 cgcccgccgc gactggtcct gggagctggt cgatcgcctc gacctgccac gctcgatcct
     2641 gccgaaggcc tacgaaagcc acgagatcac cggccggctc cgggacgaga tcgcccggga
     2701 attcggcctg ccgcgcggcc tgccggtcat gggcggcggc ggcgacgcgg tcatccagac
     2761 gaccggcagc gggctgatcg atcccggtac ggcgcagacc atcatcggca cggccggcat
     2821 cgccgcctgc gcgctcgatg cctgtgcgcc caatccgggc gggcggctcc aggtcttctg
     2881 caacaacgac ccggcgcgct ggcactgcat gggcgtgtcg atgaatgccg gcggcgcctt
     2941 cgcctggctg cgccagacgc tcggcgcctt cgccacggac gagctggaat tcgacgccat
     3001 gacggcacgt gcggagaagg tgccggccgg ggccgagggc cttttcttcc tgccctatct
     3061 gatcggcgag cgctgcccgc atcccgaccc cgacgcgcgc ggcgccttcg tcggcctgac
     3121 gctacgccac ggtgcggggc acctggtccg tgccgccatg gagggcgtgg tcttctcgat
     3181 ccgcgagatg ctggagctga tgcgcccgct cggcctcgag gccaccaccc tacgcacctc
     3241 gggcggcggg acatcgagcc cgctttggcg gcagattcag gccgacgcca ccggctgcga
     3301 ggtccagaca gtgagcgggg cggcggaggg cggcgcctat ggcgcggcgc tggtcgccgg
     3361 cgttggtgcc ggcatctggc cggacctgag cacggccatc aagggcatcg ccgtcgagac
     3421 gcggacgaca cccgacgcgg cgaaccgggc ccgctacgat gctgctttcg ccgtctatcg
     3481 cgagctctat cccacgctca agccgcgctt cgacgccatc gcggcgttgc cctaggcctg
     3541 ggcgggaaaa ccggggaagc agacgatgtc gatcgagatg tggctggcct tcgccctggc
     3601 ctcggccgtg ctgctggccg ttcccggccc caccatcatg ctggtcgtgg cctatgcgct
     3661 ggggcagggg aagcgggccg cggccgccac ggtggccggc gtggcgctcg gcgatttcac
     3721 cgccatgact gcctcgctgc tcggtctcgg cgtgctgctg gcgacctcgg cgaccctctt
     3781 caccgctctc aagctcgtcg gtgccgccta tctcgtctat ctcggcgtcc ggctctggcg
     3841 ggccccggtg atggcggccg actccggcat cgcgacgcct gtggtgagcg gccggcgcat
     3901 gctcgcccat gcctggctgg tcacggcgct caacccgaag agcatcgtct tcttcatcgc
     3961 cttcgtgccg cagttcctcg attccagccg gccgctcttc ggccagatgg tggtcatgga
     4021 ggtgaccttc gtcctcctcg ccaccctcaa cgccgccgcc tatgccggcc tcgccaccag
     4081 cgcccgccgc ctgatccgcc agccgacggc gcagcgcctc gtcaaccgcg tcggcggttc
     4141 tttcctgatt gccgcgggtg tggcgacggt cgcctggcgg cgcgcggcgt cctgatcaga
     4201 tcaccgcgag caggccgccg tcgacgtaga ggatgtggcc gttgacgaag cgcgaggcgt
     4261 cggatgcgag gaagatggcc gcaccctgca gctcctcgag ctcgccccag cggccggccg
     4321 gggtgcgctg ctcgacccag gcgttgaact tcgggtcctc gatcagcgcc ttgttgagcg
     4381 gcgtcctgaa gtagccgggg ccgatgccgt tgatctgcat gccgaaacgg gcccagtcgg
     4441 cgcacatgcc cttggtcagc atccgcacgg cgcccttcgc cgtggtgtag ggggcgatgg
     4501 tctgccggcc ggcctcgctg gtgagcgagc agatattgat gatcttcccc tggccgcgcg
     4561 ggatcatctt ccgggccacg gcctggccga catagaagac cgaatcgata ttggtcttca
     4621 tgacctcgtg ccaggtctcg gccggcatgt cctggagggg tgcccggcgc tggatgccgg
     4681 cattgttgac gaggatgtcg atcggcccct cgtcgcgctc gatcctgtcg atcgccgcgg
     4741 tcacggccgg ctggtcggtg acgtcgaaga cgtgcgccgt ggccccggga atgtcggctc
     4801 tggcggcttc gagcttggcg ccgtcgcggc cgttcaggac gacgctggcg ccggctgcgg
     4861 cgaggccccg ggcgatggca ttgccgatcc cctggccgga gcccgtgatg agcgctcggc
     4921 gcccggcgag atcgaacagt gccagcgaca ttctcgtccc tccctggtga cgtgaggctc
     4981 gtagtgctta tctggcaggg aaggcaaggc cgctgtgccg gaggggagtg acgcgccatg
     5041 gacaggaagc agcagatcaa tctgggctac gcgctgctcg cgttcctcct cctgatgatg
     5101 ttccagggct ggtgggcggc gtcgaggcaa atcgagacaa tcccgtacag cgagttccag
     5161 cagctcctcg acaagggcgc catcgacgag atccgggtct cgaccgacta catcgagggc
     5221 aagctaagga cgccgaccga gtccggtgcg cagtactttc gcacgacccg ggtcgagctc
     5281 gacatggcgg atcggctggc cggtcacgac gtccgcttcg ccggggttat cgaaagcaca
     5341 ttcctgcgcg atctcctgtc ctgggttctg cccatcctgc tgctgatcgg cttctggatg
     5401 ttcatgatcc gccgcttcgc ggagaagcag ggcttcggcg ggctgatgca ggtcggcaag
     5461 tcgaaggcca aggtctatgt cgagaaggac accaaggtga gcttcgccga tgtcgccgga
     5521 gtcgacgagg cgaaggagga gctgcaggag gtcgtcagtt ttctcaagga cccccagacc
     5581 tatggtcggc tgggtgccag gattcccaag ggcatcctcc tggtcggccc gcccggcacc
     5641 ggcaagaccc tgctcgcccg ggcggtggct ggcgaggccg gcgtgccgtt cttctcgatc
     5701 tccggctcgg agttcgtcga gatgttcgtc ggcgtcgggg cggcccgggt gcgcgatctc
     5761 ttcgagcagg cgggcaaggc caagccctgc atcatcttca tcgacgagct cgacgcgctc
     5821 ggccgggcac gcatgtccgg ccagttcggc ggcggtggcc acgacgagaa ggagcagacg
     5881 ctgaaccagc tcctggccga gctcgacggc ttcgatccct cggagggcat cgtgctgctc
     5941 ggtgccacca accggccgga aatcctcgac ccggccctgc tccgcgccgg ccgcttcgac
     6001 cggcaggtgc tggtcgaccg cccggacaag accgggcggc tgcagatcct cgaggtgcac
     6061 atcaagaagg tgaagaagct cggggcggac gtctcgctcg agcaggtggc ggccttgacg
     6121 cccggcttca ccggcgccga tctcgccaac ctcgtgaacg aggcagcgct cctcgcgacc
     6181 cgccggcggg ccgagcaggt gaccatggag gacttcaaca acgccatcga gcggatcgtc
     6241 gccgggctgg agaagcgctc gcggctgctt cacccgcacg agcgcaaggt ggtggcctat
     6301 cacgagatgg gccacgcgct ggtcgcggcg acgctgccga ccacggatcc cgtgcacaag
     6361 gtctcgatca ttccgcgcgg catcggcgcc ctcggctaca cgatccagcg gccggaggag
     6421 gagcgcttcc tgatgaccac gaaggagctc gaggagaaga tggcggtgct gatgggcggg
     6481 cgcgccgccg agcacgtcgt cttcggcgag atctcgaccg gtgccgccga cgatctcgac
     6541 aagatcagcg acatcgcgcg gcacatggtc acgcgctacg ggatgcacga tgcgaccgcc
     6601 caggtcgtct acgacccgca gcggcagtcg ttcctcggcg agaacggcat gccggtggcg
     6661 cggccgcgca attacagcga ggccacggcg cgcgagatcg acctcgccat ccgcgacctg
     6721 gtcgaggggg cgttcgcgcg ggccgtcgac atcgtcaccc agcggcgcgc cgatctcgac
     6781 cggggtgcgg cgctgctgct cgagcgtgag acgctgacca gcgccgagtt cccgctgctg
     6841 cgccgccaga agctggccgc ctgagaccgg agaccccgaa tcatgcgcaa attcctcgtg
     6901 accgtgctgc tgatcctcgt ggggctggtc gtggtggcgg tggcgctgcc cttcttcctg
     6961 ccgcgcgaga tgatcaagac ccagctcgag gggcgggtct cggcggcgct cggccgcgag
     7021 ctcgtggtcg agggcccgct caccttccgc ccctggcggc ccttcgcctt cagcctcgcc
     7081 gacgtgcgcc tcgccaaccc ggcctgggcc gagaacccgg acctggcacg ggtagcgctg
     7141 gtcgatgtcg agatcgatgc gctggcctgg ctcggtggca cgatcgatgt cgtccgggtg
     7201 gtcatcgacc agcccgtgct cgccctcgag gtcgatgcgg aggggatgcc gagctggcgg
     7261 ttcgaggggc ccgacgaatc cgacgacagc agcggcgacg gcgacgggga ggcatcggat
     7321 ctgcccgacg tgcggatcgg cgacgtcgcg ctgaccgatg gtgccgtcac ctatatcgac
     7381 catggcaccg gcgagcggcg cgagttctcg ggcatcggcc tcggtgcccg caccgaagcc
     7441 gaaagtggcg cactcctcct cgatggcgcc gcgacgagcg ccggccagca ggccacgctg
     7501 gagggccgga tcggcgatct cgccgccctg ctcgacggcg agccgagctc tgtcaacctc
     7561 gagcttgccg cacccgggct cggcctcgtc gccggcggcg aggcgtcgcc tgcgggcacg
     7621 gccgtgctcg ccgtcacggt cgacatggcc cccaggaccc tcctcgactg gctcggccag
     7681 ccggtggcgc tgccggaagg caggctcgag agcgccacgt tcacggtcga tctcgaatcc
     7741 gcgccggaca gcctcggcct gcacgcgctg agcctcgccg tggacgagct cctggtgcag
     7801 ggcgatctcg acctggcgtt cggcgagcgg ccgcggctgg ctggccggct cgatctgggc
     7861 gatctggacc tgcgccccta tctgccggcg gagaccccga cggacggggc tgcggcggcc
     7921 gcggccgacg cgccggcggc cgaggcggat ggctggtcca ccgagcctct ggacctgccc
     7981 ttgccgctgc cgctcgacct ggacctcgag ctcgtgatga attcgctgcg cgaggccagg
     8041 ctcgagaccg gtcccggacg ttttctcgcc caggccgacg ccgagcgggc ctcgctcgag
     8101 atcgccgagc tcgccctcta tggcggcggc gccaccggga ctgcggcgct gacctcggac
     8161 gatcccctga aggtggaggc gagcctcgac gcgaccgggg tcgagctcct gccggtgctg
     8221 cagggggctg ccgatatcga ctatctcgaa ggcaccggca atgtcaggtt cacgatcgag
     8281 gggctgggcg acagcgtcgc caccctggtg gagagcctcg ccggcgatgg cgaggtcctg
     8341 gtgcgcgacg gggccatcct cggcatcaac atcgccgcca ccatccgcca ggtgatgacg
     8401 ctcggggtcg aatcggcggc cacgcagccg cagcggacgg acttcgccga ggcgggcggc
     8461 accttcacga tcgagcgggg catcgtccgg aacgacgact tcaacctgcg ggcaccggtg
     8521 ctgcggatcg agggcgccgg cgaggtggcc ctgccgccgc ggacgctgga ctatcgcctg
     8581 acgccgcatg tggcctccac cctccagggg caggatgcgg gcggcgagcc ggacctcagg
     8641 ctcggcgtgc cgctggtcat cgagggggcc tgggcgagcc cggccgtcag gctcgacctc
     8701 ggcggtgcgc tctccggcga catcaccgac ccgtccgagc tggccgagac ggtggcccgc
     8761 ctcgccgccg acccggcgaa tctcgaggcc ctgaaggaga tgttcggtgg tgacttgccg
     8821 ggctcgctgg gcgatgcgct ggagcagctg ccgggcgtgc tcggcggatc ctcgggcaat
     8881 gacgacagtg aagcaggggc accggcgaag cggctgcgcg atgcgctcgg cggcctgctc
     8941 ggtcgctgac gaactccggc gagaaacgga accctcgatg atcgatctgc gcagcgacac
     9001 gctgaccagg cccacgccgg cgatgcggcg ggccatggcc gaggccgagg tgggcgacga
     9061 cgtctatggc gaggacccga ccgtccgccg gctggaggag cgcaccgccg ggctgctcgg
     9121 caaggaggct gccctcttcg tgccgagcgg caccatgggc aaccagctcg ccctgcgctg
     9181 ccagaccgag cccggtgacg aggtgctgct gcacgaggcg gggcatatcg ccctctacga
     9241 gcggggcggc tacgcgctgc tctcgggtgt cgtgctccgg ctgctgccgg gcgagggcgg
     9301 cgtggtgaca cccgcgacgg tcgagggcgc catccggccg gccgccatcg cggcctacgg
     9361 catctacccc ccggcccgcc tgctggcgct cgagaacacg cacaatgcgg gcggcggcac
     9421 ggtctggccg cccgagctcc tggcggcgac cgcggcggcc ggccgggcgg cgggccttgc
     9481 cttgcatctc gacggcgccc ggctgtggaa cgcggcaatc gcctcggggg cgagcccggc
     9541 ccggctggcc gcgcccttcg acacggtgag cgtctgcttc tcgaaaggcc tgggtgcccc
     9601 ggtcggctcg gcgctcgccg gcaggacgga cctgatcacc aaggcgcgcc ggtttcgcgg
     9661 cctcttcggc ggcggcatgc gccaggccgg cgtgctggcg gcggccgccc tgcacgccct
     9721 cgaccaccag cacgagcgcc tcgccgacga ccacgcggcg gcgcggcggt tggccttcgg
     9781 gctcgccgag ctcggcccct ttcgcctcga cccggcggcc gtgcgctcga acatcgtcat
     9841 tgccgaggtg gtcggtcgac cggccgccgg catcgtcgcc gcggcacgcg agcgaggtct
     9901 tctctgcctc gaggtcgggc ccggtcgcct gcgcttcgtg acccatctcg acctgaccgg
     9961 cgacgccgtc gaccgggcgc tggcgattct cgcggatgtg gtccgggagg tttcctgaca
    10021 ttgctgcgga atggccgcgc ggcatcccgc gaccgcgggt cgcgcatggt gtttgaactg
    10081 cggcccgaat aacggctatt gacggggagg tgccggttgg ccgacgcctt tgcagacggg
    10141 ccgatttcgc gtccaagggg aattatgcgg gttcttgctt cgtggtttct ggctgccggt
    10201 ctggcaattg gtggggccgc catcatcccg atcccgtcat ggatcgggag cgcagcggcc
    10261 cagacgttcg ccgtcggtga gggggcgcgg gtgctcgggg ccagtgacac acggatcaat
    10321 gtccgctcgg cgccgcgggt ggcaagcggc aacatcgttg ccagcaccaa gggcggcgag
    10381 ctgctccggg tgctggaggc ggccgaggaa ggccagtaca cctggtatcg ggtgacgacc
    10441 ctgcccggct cggccgagtc gttcgaaggc tggatcagag gagatctcct ggcgtcggcc
    10501 gccttgcccg agccgaccct gccgcaggtc ctggctacac ccgtgccggc tgccgacgtt
    10561 cccgacgcgg cacggcgtgc cagcgaagcg gcggcgccgg tgccactctc gcaacgcacc
    10621 gactggtcgc gcgatctgct ccggctcttt cccgcgatcg agggctgcgc cgccgtcggc
    10681 tcggcgccgc cgatcacggt gctgcgggcc atcggccgct cgcgggggct cgccgagatc
    10741 atcatgagcg atgcggcggg acgccgctgg gactgcgtca ttcgtgagac cggcggcacg
    10801 ccgatgcgct acgatccgct ttccggcgcc gtctacatgc gcgatcgcat gggcaacgag
    10861 ccgttcttcg ccataggcga ggagcgtccg gcgctcgatc cgaactgctt ccgcttcgag
    10921 cgggtgatcg atccgacgaa cggcgagatg ctgggctggc tctactaccg gacctgcccg
    10981 tgagcggcct cgtcgagctc ggcgccaccg ccgcggtcgc cgagctgcgg gcggggcggg
    11041 tcacgccgct ggagctcgtc gaggcggcga tcgagcgcat cgagcggctc gaccccctgg
    11101 tcaacgcggt gccgatccgc tgcttcgacc gggcgcgcgc gcatgcccgt tcgctcatgc
    11161 aaggaacggc accggccgag cccgggcgcg gctggctcgg tggcctgccg atcgtcgtca
    11221 aggatctctt cgaggtcgag ggggtcagga cgacctacgg ctcgcccatc ttcgccgagc
    11281 atgtgtccga ggtcaccgac ctgcatgtgg ggcagctcga ggccaacggc gccatcgtcc
    11341 tcggcaagtc gaacacgccc gagttcggcg ccggtgccaa caccttcaac gaggtcttcg
    11401 gcgccacgcg caatccctgg aacacggcgc tcacctgtgg tggctcgtcc ggcggctcgg
    11461 cggtggcgct ggccacgggc atggcgtggc tggcggatgg cagcgacctc ggcggcagcc
    11521 tgcgcacgcc cgccgcgttc tgtggtgtgg tcgggctgcg cccgagcatc ggccggatca
    11581 tgcgcggccc ggcgtcgcgc cgcttccaga cactcaccgt caatggaccc atggctcgcg
    11641 atgtggccga cctggcgctg atgctggacg cgatgagcgg cacctcgctc gatgacccgc
    11701 tggcggcgga agcgccggcc atgtcgttcg ccgccgcggc tcggatgccg cctcgcctgc
    11761 agcgggtggg gttttcggcg gatctcggcg gcttcgtgcc ggtcgacccg gagatcgccc
    11821 ggctcgccgg gcaggcggcg gcgcgcatgg ccgagctcgg tgctgccgtc atcgagaacc
    11881 gctcgccgga cttcggcccg gcccgggagg tcttcgaggt gctgcgcgcg gcccacttcg
    11941 cggccgagaa ggcgccgctg ctcgaggccg agcgggacaa gctgaagccc gaggtgatct
    12001 ggaacatcga gcgcggcctg gcgctgaccg ccgacgagat cggctgggca gaacgcgagc
    12061 ggagcctgat ccaggcgcgg gtcgcggcgt atttccgcga gcatgacctg ctgctctgcc
    12121 cggccgccat cgtcccgccc ttcccggtcg agctgcgcta cctcgaagcg ctcggcgagc
    12181 atcgctttcc gacctatatc gactgggtgg ccgtgaccta cgccatcacg ctcagcggct
    12241 gtccggcgct gtccctgccc tgcggcttca ccgaggccgg cctgccggtc ggcctgcagc
    12301 tggtggggcc accccagggc gaggctcggt tgctcgcggc ggcgcgtgcg ctcgaggagg
    12361 tcctggcggt cggctcggcc cggccgatcg agcctcgctc gccgctcgcc ggggcctgag
    12421 caccggcccg ttcccggccc tgcgccattg tggcagaagg tggccctggg ctcgctgacg
    12481 actggagaga agtgatgttg cggatctggg ggcggacgaa ctcgatcaac gtgcagaagg
    12541 tgatgtgggc ggtgggcgag ctcgatctcg cccatgagcg gctggatgcg ggcggcgcct
    12601 tcggcgggct cgacggcgat gcctatggca ggctcaaccc caatcgccgg atccccgtgc
    12661 tcgaggatgg cgagacggtc gtctgggaga gcaacgccat tgtccgctac ctggccgccc
    12721 gctacggcgt cggcacgctc tgggccgagg atcccgccct tcggtcggtc gcggatcgct
    12781 ggatggactg gcaggtgacg accctgctgc cgccgctcca cccgatcttc tgggggctgg
    12841 tccgcacgcc cgggccggaa cgcgacatgg cggcgatcga cgctgccgcc aaggcgatcc
    12901 agccgctctg gcagattctc gacgagcatc tgtcgaagag ccgcttcatc gccggcgacg
    12961 cgttctcgat gggcgacatc cccctgggct gcgccttctg gcgctatgcc aatctcgatg
    13021 tcgagcgacc gagcctgccc aatgtccagc tctggtacgc cgagctcaag aagcgcgagg
    13081 cctatcgccg gcatgtgatg ctgcccgtga cgtgaccgcc gcgggcgacg tcgccgagga
    13141 ttcaccgccg ccggaagccc cgccaccgag cctgcgcgcc ggcctgcgcg acctgtatta
    13201 tggcgccgac cggcgggccg cgcgcttcca gtacgggctc ctgctcttcg acgtcgcgac
    13261 gatcctcttc ttcctggttg tcaccttcgt cgccgacgcg ccctggctga tctacgccga
    13321 tctcgtcctc gcggtcgtcc tctcgctcga tttcgcggcc cggctctaca tcgcgccccg
    13381 gccggggacg ttcctggcgc actgggtcac gattgccgac ctcctcgtca ttctctcgct
    13441 gatcgtgccg ctgctcaccg agaactacac gacgctggct ttcctcaggg tgctgcgcgc
    13501 ggtccggctg gcccgctcct accatttgtt cggcatgctg cgccgtcgcc acaaatgggt
    13561 gcaggagcac gaggacgccc tgcagagcgc catccacatg gtgctcttcc tcttcgtcac
    13621 cacggccatc gtctacgtcc agcagttcgg ccggaatccc gccatcggca gctacgtgga
    13681 cgcgctctac tatacggtgg cgacgctgac gacgaccggc ttcggcgata tcaccctggt
    13741 cggggagggt ggcaagctcc tctcggtgct gatcatgatc gtcggcatcg ccctcttcgt
    13801 ccggctggtg cagacgatct tccggccggc caaggtcaag caccgctgcc cggaatgcgg
    13861 cctcatccgg cacgacccgg acgccgtgca ttgcaagcat tgcggcgagg tcctcgacat
    13921 cccgaacgag ggcgattgag cagggtcagt cgggacgctg gtggttggcg ctgatcggct
    13981 tgacgtattg cggctcggtg tcccagggga acaggatcca ggtatcctgg ctgacctcgg
    14041 tgatgaaggt gtcgacgatc tcgcgcccgg ccggcttggc gtagacggtg gcgaaatgcg
    14101 ccttcggcag gcgctcgcgc acgatccgcc cggtctgccc ggtgtcgacg aggtcgtcga
    14161 tgatcagcca gccctcgcca tcgccgtcca ccgacttcag caccttcggc tcgcgttggg
    14221 tctggtcgtc gtaggaggcg atgcagatcg tgtcgatcac ccggatgtcg agctcgcggc
    14281 tgatgatgtg cgccggcacg aggccgcctc gggtgatcgc gacgatgccc ttccaggggc
    14341 cgcccgaggc cagtcgccag gagagggcct tggcgtcgcg atgcagctgg tcccatgaga
    14401 cggggaagaa tttctggtat ggatcgttca acgtcggatt cctcgggcgg cggcggttgg
    14461 ccgctagagg ccgctggcct cgccggttgt caagcggcgg gtcgccgctc gccccgggac
    14521 cgcctgaacc tgtgacggag accgcccatg gacgaggagc tgccgcggaa gaagccgagc
    14581 gagttcgcgc tgggcagcga gctggaccgc cattcgctgg acgagctcga agcactcgcc
    14641 gccatgctgc gcgccgagct cgagcgggtc ggccgggcca ttgccgccaa gcgcgatgtg
    14701 cgggccgcgg ccgaggcgtt cttcaagcgt caggagccgc ccgccccggc cgacggctga
    14761 tgcgcttctg ttcggcttgc ggcgggccgg tcgagcagcg ctgcgagggc gggccgtacg
    14821 cgccgtgggc aacccgccgg cgagcgggcc ggatggcgat tgacagcgta tgccgcccgg
    14881 gttaacgccg agccatggag aggttcgaga agatcgagcg acgacgacag gcgcccagtc
    14941 gcgccgacgt caccccgacc gcccgctggc gctgagccgg accggcaagc ggggcggccg
    15001 agttcagctt agcttcgatc ggtcccgccc atgttctcca ttcgcctcga gcagccggcc
    15061 gattcggtcg ccatcgaaga gcttctcgat ctcgccttcg gcgccgatcg gcgcaagaag
    15121 acctcctacc gctatcgaga tggcatcgca ccgctggccg acctctcctt cgtcgcggag
    15181 gccgaggccg ggctcctggt cggcagtgtc cgctactggc cgatcctcgc cgggtcgacg
    15241 ccggcgctgc tgctcgggcc gctggcgatc gagcccgcac ggcagggccg cggcgtgggt
    15301 cgggcgctgg tgttccacag cctggatgtg gcggcgcagg ccggccacag gctggtgttc
    15361 ctcgtcggcg accccgccta ttaccagcgc ttcggcttcg acccggtgcc gccgggcatc
    15421 gtcatgccga acgaatcgcc cgcccgcctt caatatcgcc tgctcgaccc gtctctggcg
    15481 ctgccggaaa aggccacgct cagacgcagc cagagccgcg ccgcgcaccg ggccgggcgc
    15541 ggcgcatcgc tcgcgggaaa ggcgattgcc ggggcatcgt gcatcccgta gattgcgccg
    15601 cgtcggggcg tagcgcagcc tggtagcgca tctgctttgg gagcagaggg tcgggtgttc
    15661 gaatcacccc gccccgacca agcacgctgt cttgcgggat ccgggcgggc gggcgtcagc
    15721 gatgcggggc cggacgagac cggcatccga gcgtgaggat gggcatggcg cagccgccac
    15781 tgcaactcgt catcggcaac tacaacacgt cctcctggtc gttgcgggcc tggctcgcca
    15841 tgcgcctggc tcggctcgcc ttcaccgaga ttcgtgtacc cttgcgtcgc gaagacacgc
    15901 cggccagcat tcgtcgctac tcgccctcgg gcaagctgcc ggtgctgctc gtcgacggca
    15961 cgcccgtctg ggattcgctg agcatcgccg agctcgtcgc cgagctcgat cctgcggtgt
    16021 ggccggccga cgccgcccgg cgcgcggagg ctcgctccat cagtgccgag atgcacagcg
    16081 gcttcgcgga tctgcggcgc ctgatgccga tggacgtggt gtcgcgattc gcgccgccgg
    16141 gccgcatgcc gcgtggtctc accgccgaca tcgcccgcat ctcggcgatc tggcgaagct
    16201 gtcgtgaccg gtacgcggcc gacggtccgt tcctgttcgg cgccttctcg ctggccgatg
    16261 cgatgatggc gccggtcgcc tcacggctcg tcacccacgc cgtgccgctc gatacgatgc
    16321 tcgacgacta cgtggacagt gtcatgtcct ggcccgacat ggtcgtctgg cgcgatgcgg
    16381 ctgacgccga ggtggaggcg ttgcgggatc gaggcgccct ggagattcag tccgtggccc
    16441 gggccggccg tgaggtcccc gacgagccgc tggggggggc gcaacttctg gccgcgacgg
    16501 cggtcgagcc gtccccgcca cggcgggtcg agccgcaagt cgtcgtcagc ctcgatgcca
    16561 cgcccgacgc cacgtctgac gaagctcccg aagccgtttt cgaagaagtg ccggccattc
    16621 cggtggagcc gtccgccatg ccggaagccg cgccggcgcc acagggtcgg gccgagccgc
    16681 ctcgccagga tgcaagcgag caaatggaga cgcccgccat cgctgcatcg ccttcgcctg
    16741 cggagcaaca gtcgccgccc gccgacgagc cgccgccgcc cgccccgccg ccggcccgaa
    16801 cggccccggc cccttccgcc gcatcagcgc cgaggcctcc cgtggtcgag cctgcgacgc
    16861 gtcctctgcc ggaggcgacg ccggaagccg gtgctcaggt cgaggcgccg cggcgccccg
    16921 ggggaacgac gatcaagccc ataggcagcg gaattcttcg acgacgctga gatagacgtc
    16981 gcgcttgaag gggacgattc gctcgaccag ctcgtccggc gccagccagc gccaggcgca
    17041 gaattcgggg tgctcgccgt cgagcatgat gtccgcatcc tcgccgaggt agcgaaaggc
    17101 gagccatttc tgggtctggc cgagatagcg gccgcgccag aggcggcggg cgatctccgg
    17161 tgggagatcg taggagcgcc agagggcgct ctccttgagg aggtcggccc ggttggtgcc
    17221 cacctcctcc tgcagctcgc ggcgggccgc gtcgagggcg ttctcgccct tgtcgatgcc
    17281 gccctgcggc atctgccagg cttcggcccg ggtatcgtgg cgctggccga cgaagacccg
    17341 gttggcccgg tcgagcagca tgatcccgac acagcgtcga tagcccggca aggggccctt
    17401 gctgacccgg cagctcatgc gacggcggcg aagggggccg gccatgcgct catggctcgg
    17461 ccaggaaccg gctgaccggc acgagccgga tacccttggc ctcgagaccc tgcgcccaat
    17521 gccagagccg gtcgaacgtg aggggatagg gctgggcgta ggccagggcc cggccgtcgc
    17581 gccgcgctgt cgcttccaga cgggcgagcg ccgcatcgat ggcggcggcg tccggaaccg
    17641 cgtcgagcgg gccgctggcg cgtgcatagg cgacgccatt ggccgccgcc gtcgcggcga
    17701 gcagcggctc gccgagctcg accagggcga gcccgcgggc ggcgagctcc gcggcgaccg
    17761 gcgtgaagcg ctccggggca gcggcgaagg cgccgctggc ggccacgagc ccgagatagg
    17821 cttcgccgcg gctggcgacc cgccgggtgc cggcaatgag ctccgggggg ggcttcgagg
    17881 gatcgagcgc ccaggggccg aaatcgctgg tcgggtggtc ggctgcctgc agcggcagct
    17941 cgagcagcac ttcgtggcca tgccagcggg cgtgccgctg ccagctcggc agatgggccg
    18001 cgtagggtgt gtgggcgagg ccgatggcgg ggggcagggc gacgctctgc gtcaaccgct
    18061 ccgcatcgag gccgagatcc gtgaccagga tggcgatctc gatgggcgat gccgccggaa
    18121 cggcggtggg gtcggcgccg cgcgcgtaga gcatgcgcgc ctgccgcccg tcggcggcaa
    18181 tccgcggcag ggtgagcccg tcgatcgtct cgacgagatc cgggtcgacc tccagccggg
    18241 gggcctccgg atcgggcgat ggcaggctcg agaggggcgg cagcacggcc gggctagcca
    18301 gcccgaagag cgccggcggt cggggtcgcc gggcttcggc gagcggcccc tgcacgctgg
    18361 gcaggccgac atgacggtag ggcgcctcat gacccgtcag caggcttgcc gacgccacaa
    18421 gcaggctgcc gccgagcatg gcggcgccga cgacgagcgg caggcgcggc gagcgccagt
    18481 cgaccgcggg caggaaggcc ggcggtagca ggcgggccgg caacggcatc attgcccgaa
    18541 cccaccgggc cgcatgcact cctgccacgc gcctgccaac cggctcggcg gtgttcccgg
    18601 ctccccgcaa gggtcagccg cgctgcgtcg cacgggcctc gtaaagcgca atgccgcgca
    18661 gcacgtcgat ggcccgggcg agctggtagt cggtgctgtc gagggtcggc tccgcctcgc
    18721 ccgacgcggc ctgatccccg ggctgctcgc cgtcgagccg accccgcaga tccgcctcgc
    18781 ggcgtcccga ggtgtcgtcc tgcagctcga tggtcgcctg gtgcacctcg atgtcgggag
    18841 cgatgccctt ggcctggatc gaggtgccgg agggcgtgta gtagcgggcg gtggtgagcc
    18901 ggatggcgcc ttcgccggag atcggcatga tggtctggac ggaacccttg ccgaaggact
    18961 gcgtgcccat gatgatgctg cgccgatggt cctgcagggc gccggcgacg atttccgacg
    19021 ccgaggccga gccgccattg atcagcacca ccatcggcag gccgtcgatg atgtcgccgc
    19081 tgcgggcgtt gaaccgctgg atgctctccg aacggcggcc gcgggtcgag acgatctcgc
    19141 cctgatcgag ataggcatcg gccacggcca cggcctggtc gagcagcccg cccggattgt
    19201 tgcgcagatc gagcacgagg ccgcggaaac ggtcgccgag ctggtcgcgg agcttcgcca
    19261 cctcttcccg catgatgtcg ttggtctgct cgttgaaggt ggtcaggcgg acatagccga
    19321 tatcgtcctc ggccctggcg cgcaccggcg agatcttgat gatggcccgt gccaggttga
    19381 ccgagagggg ttcctcgcgg ttctcgcgca cgatcttgag ctcgatctcg ctgttcaccg
    19441 gcccgcgcat gcgctcgacg gcctcgctga gcgacaggcc cagcaccggc tcgccatcga
    19501 tatgggtgat caggtcgccg gcctggatgc cggcatcgaa ggcgggcgtg tcgtcgatcg
    19561 gcgagacgac cttgaccagg ccgttctcca tggtgacctc gatgccgagc ccgccgaact
    19621 cgccccgcgt ctgcacctgc atgtcgcgat agcggccggc gtcgagatag ccgctgtgcg
    19681 ggtcgagcga ggtcagcatg ccgttgatcg ccgactcgac cagctcctgg tcggtcacct
    19741 cctcgacata ttccttgcgc actcgctcga acacatcgcc gaacagcttg agctgccgga
    19801 tggtgtcgcc cgagccgctc tgcgcctggg cggaaggcgg tggcatcatg cacccggtga
    19861 tcatcaggac aaccaccaca gcggtgccga tccggcctgc caatcttgtc atccgcgtac
    19921 cttatcttgt ccggccgcca accacggcag cgggttcacc ggaacgccac gtcgccgaag
    19981 ctcgacatag agctgagccg tgccgccttg ggtgggttcg agcgtgccga ccatctgccc
    20041 ggcgcgaacc gcagcaccga ccgacacggc gagtctggaa agaccagaaa gcagactatg
    20101 atattcgtct ccgtgctcga tgatcaagag gagtccgaag cccttgaagg gaccggcgaa
    20161 ggcaacccgc ccatcgcgcg gtgcccggac cagtcttcgc tcctcgaccg cgatggttat
    20221 gccctggtcg agcagggcct tgggcccctg gccgaaatgg ccgacgaccg agccggcgac
    20281 cggcatgacc tggcccatgc cggaaagggc ggcaacggcc gaggccgcag gctcgccgaa
    20341 gagcaccgcg acacggtcgg gttggcggtc cagcacggcg cgagccgtca gcaccgacgg
    20401 cttcagccga ctggcggtcg ggccgaggtg tgccgggctc gggagtcggg cagcggcatc
    20461 cggcaggctg gcgagcagcc gcgattccag cacccgctcg tggcgcgcca gggcgcgctg
    20521 cgcatcgcgg cgcgccgcga gctcgcgcct cgtcgcctcg gccaggtcca ggcggatggc
    20581 acgctccgcc gcctcgagcc cggtcgatcg tcgttccagc gccagttgcc gggagcgcac
    20641 gcggaggccg gctgcccgca gctcgagctc ctcgttcgcg aaggccagtc gggcggcacg
    20701 gctcgctgcg agcgcctgct tggcttcggc aaatggctcc acgtaggaag cagcgatcgc
    20761 gcgcagctgg gcgatccgcc ggcggtcggc cacctcggcc cgatcgagtt gcatgagctc
    20821 ggcgagcacg gcttcgagtc gccgctggag cgccagcacc cgctcgcccc ggctggtcac
    20881 ggccgtggtc gcctcgtccc gcctgtccat gagcccccga aacgataccg cgagatccag
    20941 aagctcctgc tcgagggatc gcagctcccc ggtcgtgtgg cgtcgcgctg cgtcgagctg
    21001 cgccagcatc tcgaccaggc gctcggcggc gaaggcgcgg tcgtcggccc tggccgccgc
    21061 catcgccgcc acggcggcgg ttcgctcggc ggtctcgacg gcgacaacac cggccgaaac
    21121 cgactggccc agggcgagca gaagcaagct tgccaccagg acggggcgca ggccgccgtg
    21181 aacggaacgg tcgcggccgt caggcacgtc gggcgatcag gctgtggccg gtcatcgccg
    21241 ctggcttcgg gatgtcgagc agggccagca gcgttggcgc gacgtcgccg agccggccgt
    21301 cgcgaaggct ggtgccggag gcggcgccga ccaggaagca gggcaccggg ttggtggtgt
    21361 gggcggtatg cggaccgccg gtcgccgggt cgcgcatcag ctcgcaattg ccatgatcgg
    21421 cggtcaccag gatggcaccg cccacaccga gcaccgcctc gatgatccga ccgaggcagc
    21481 gatcgaccgt ggccacggcc cggatcgctg caccgagcac acccgtatgg ccaaccatgt
    21541 ccggattggc gaaattgacg agaatgaagt cgaaccgttc ctcaccgatc gccgcgacac
    21601 aacgatccgc cagctcgggc gccgacattt ccggctgcag atcgtaggtc gccaccttgg
    21661 gtgaagggac cagaatccgt tcctcccccg gatagacccg ctcctcgccg ccgttgaaga
    21721 agaacgtcac atgcggatac ttctcggatt ccgccatgcg cagctgcttc aggccggccc
    21781 gggcgacgac ctcgcccatc aggtcatcgg cgggctgggg cggaaagaga acgagcatgc
    21841 agcccgccag gtcctcggcg tagtgcgtca tgccggccag gaccgagagc ttcggccggc
    21901 gcacccgggc aaagccgtcg aaatcctcga ggcagaaggc gcgaagcagc tgccgcaccc
    21961 ggtcggcgcg gaagttgaag cagagaaggg catcgccgtc tgccatgccg gcgtagtcac
    22021 tgatcaccgt ggcgggcacg aactcgtcgc cgacgtcccg ggcatagctc gcgcggatgg
    22081 cgtcgatggc gctcgcggcg cgcatgacgc cctcggccgc cgtgtccctg cctgccacca
    22141 tcgcctcata ggcgacgcgg gtgcgctccc agcgacggtc gcgatccatg gcatggtacc
    22201 gaccgcatac ggtggcgatg cgtgcgtccg gcaacgggcc gagcaccgac tccagcttct
    22261 cgatcgacgc tgcggcggat cgcggcggcg tgtcgcggcc atcgaggatg gcgtgaacgc
    22321 gcagccgcag gccagcttcc gccgcggccc gggcaatggc gaagagatgc cgctcatgcg
    22381 catgcacccc gccgggcgac acgaggccaa ccagatgcag gcagccgctg ccgccacggg
    22441 cgctctgcac cagctcgacg aacggcgttg cctgccagag ggagccgtcc tcgattgcgg
    22501 cgtcgatgcg cggcagctcc tgcagcatca cgcggccgcc gccgaggttc atgtgaccca
    22561 cctcggaatt gccgaactgg ccttccggca ggcccaccgc cttgccgtgg gtggtgagaa
    22621 ggcaatgtgg tgccgcggcg aaccacttgt cgaacaccgg cgtctcggcc aaggccaccg
    22681 cattgtcggc ggactcgcga cggtagcccc atccgtcgag aatgcaaagc acaacgggcc
    22741 gtcgcatcac gtccgagccg tcactgtcac ctgtcgcata gcatcgtgcc accgacctgc
    22801 tgagctgcat ccgggagaat agccaacccg accccacatc gccagtcttc taacgatgtt
    22861 agcggcggtt tcttaaccgc ggcctaacgc aagggcggtg ccgatgtccg tcagttcgcc
    22921 gtgcgtggtt cgccgccatc gcggcggcca ctgaagacgc tctcgacgaa ggccccgaag
    22981 ctcgcatagg tggccggtgc cgaggaatca cgcaccgttg gctgcgactc cggcccggcc
    23041 gctgtggacg tgacatccac cggttggccc ccagcgagct cggcgacgat tccggcaacg
    23101 ctgggcccgg aagcggcggc caactgcgtg gccggctcga gtgccgcagc ggctgcggcc
    23161 tccaccggct ggtcggatcc gtcgatgagc ggcgccatgt cgagcgcatc gaagggcggc
    23221 tcgcctcttt ccttcccacg caccgggcgc ggctcggagc gcgggtcgag gatcggcgcg
    23281 ctcttctgct cgatggcttc gggcgtctgc tcgtccgccg gcttgcgatt gatcgagatg
    23341 acctcgcgcg ggcgcgcgat ctcgagcgtg tcgagcagct cgccgacgac ggcgagcacc
    23401 cgatagacgg cgaaccgctc cgtgtacgtc gccgtgatga ggctgaccct gtcgatgaac
    23461 agctcgttct cggcatcgag cagatcgagc agctgccgct ggccgatctc gaactgggag
    23521 gcataggcgt cgcgggtgcg ccgctgcgcc tcgaccttgg ccaccgacgc ctcgatccgg
    23581 tcgcgggccg tatccagggc gttgtacgag atccgcgcct cctgctcggc ttcgcgccgc
    23641 gtgttctcga gcgcgacgcg cgcctcgttc atgcggtgaa aggcctcgcg ctcgagcgcc
    23701 atgtcggcgc cgccgcgata gaggttgtag cgcatgacga ggagagctcg ggcgtcgttg
    23761 tcgcggcctt ccgcgccatc gatgtcgtga ttcagcccgg caccgacctc gacgtcgaaa
    23821 cgcggataga agccggcgcg cgagccccgc gtctcggcgg ccgccacgtc gacgtccgcg
    23881 gccgcgatgc gcacggtggg gctggagacg gaggcgagat tggcggcgtc ctcgggcgac
    23941 ggcggcaggg ccgcgaccgg cgcctcggaa tcttccagcg aacccggccg gaccccgaca
    24001 acccgctggt atacggcgat cgcatcggcg agcgagccgc gcgcgatggc cagcgtgtcg
    24061 aaggcgcggg cgagccgggc ttcggtctgc cggacatcgg cgatgtcgcc gcgcccgctc
    24121 tcctcgaggt ccctgacctg gccgaggatc cgctcgtgct ggacgatatt ggcctcgttg
    24181 aggcggacga tctcctgatt gcgcaggacg ttgaggtgcg cctcgatggc attgacgccg
    24241 acgaactctg ccgcctcctc gacccgatag gcggcgctgt cgacccgggc acgctggcga
    24301 tcgacctcgc tctgcgtggc gaaaccgtcg aacagcatct gcgtcagcga aagatcggcc
    24361 tccgagcgca gcttggtctc ggagccgccc tcgaaggacc gcgtggtgaa gttgtccgac
    24421 cactcgggcc cggcagcgcc acgaaaatcg atcgacggca ggtagccggc ccgggcctgg
    24481 cgcagctcct gatccacggc cttgcggttg ttctggacga tcccgacctc ggggttcgtc
    24541 tccagggcca actgcacgct ctcggctatg gtgttcgccg aggccatgcc gctgccggca
    24601 acgacagcca cggtcgccat cagcgccagg gtccagggtc gaaggcctat ggagccagtt
    24661 cgtgtttgca tgtgcccatc cgctggtctg agtcggttgt cgcagctcaa ccgtggctta
    24721 atgcaggtgc atcgggtgta atcgtttagg atattgagcg tcaacgcgat tccggccacc
    24781 tccggggagg ctgcgtgggg tgtgattttt ttgcctgagg gttgcgcgac ggggcaaaag
    24841 tcgctgcgtg acacgaaagc ttaactgttg gatagcatgc tccagaacag ctatccgggt
    24901 ttgccggacc agtgcggtcc gggcaatggc gcgatggaga ggtggatgac ggacggcgca
    24961 atgttgttgc gcgaacggga cacccgggag gtcgatggcg gcgggcgggc cgagggcgcc
    25021 ggctccggcg accgcccacc ggtggaggcc gcctcctggc agatccgcga gagctttgcg
    25081 acacgcgacg attccctgct ccgtgccctc gagacactca cccagctgca cgagcggccg
    25141 acctccggcc aggcgctggg tgccggcctg ccgctgccgc ccgacggcct gatgaccccg
    25201 gcactggcgc cccgggcgct cgagcgggcc gggctgagcg cccgtctcgt gcgccgcgcg
    25261 atctcggcca tcgatgccct gtcgatgccg tgcatcctct ttctcaagga tcggcgctgt
    25321 gtcgtcctcg tcgagcgtcg gggcgcggtg ttgcgggtcg tcgtgcccga gacggggcgg
    25381 ggcaccgtcg aggtcgagct cgcggatctc gagcgtcgct acgatggcca cgccctcttc
    25441 gcgcgtccgg agatcggtta cgagcagcgc gatcggccga ttgccgagga gcgcaagggg
    25501 cactggttct ggtcgacgct gctgcgccag tggccgacct acgcggaggt catgatcgcc
    25561 gcggtcatga tcaacgcctt cgcgctcgct tcgccgctct tcgtgatgaa cgtctacgat
    25621 cgcgtcgtcc ccaacaacgc cctggacacg ctctgggtgc tggcgctcgg tgcgttcatc
    25681 gtcttcgctt tcgatttcct gctcaagagc ctgcgcggct atttcatcga caccgccggg
    25741 cggatcgcgg atatccgcct cgccagtcgc atcttcgagc aggttctcgg catccggatg
    25801 gcagcccggc ccgcttcggc cggcgccttc gcgaacaacc tgcgcgagtt cgaatccttg
    25861 cgcgacttct tcacctcggc gaccgtgacg acgctggtcg acctgccctt cgtcctcttc
    25921 ttcatcacca tcatctggct gatcggcggg ccggtggcgc tcgttccggc aatcgccgtt
    25981 ccggtggtga tcggcgtcgg gctcgtcatg cagatcccgc tgaaccgtgt ggtccgccgc
    26041 acgttcaagg agtcggcggc caagcatggc gtgctggtcg aggcgatcaa cgggctcgag
    26101 accatcaaga gcatcggcgc cgagagccgg ctgcagcgcg gctgggaacg ctatgtcgcc
    26161 gctaccgccg agagcgccaa ccggtcgcgc ttcctctcgg ccatcacggt caacgtcgcc
    26221 gggctagccg ccaatctggt gacggtcggc gtcgtggtcg tcggggtcta tcggatcggc
    26281 gagggcctca tgaccgtcgg cgcgctggtc gcctgcacga tcatcgccgg tcgcgcgatg
    26341 gcaccgctcg gccaggtcgc ggggctgctg acgcgctacc atcaggcgag gatgtcatac
    26401 gacacgctca atcgcgtcat ggcgatgccg atcgagcgtt cgccggacat gcgcttcctc
    26461 gaccggccgc ggctctccgg ggcgatcgag ttcaagaacg tgacgctcac ctatcccggc
    26521 cagaagctgc cggcgctttc ggatgtcggc ttcaggctgg aggcgggcga gcgggtcggg
    26581 ctgatcgggc gcatcggctc gggcaagacg accatcgaga agctcgtgct cggcctgtac
    26641 tcgccggaga agggggccgt gctggtcgac gggaccgatc tgcggcagct cgatccggcc
    26701 gatctccggc gcaatatcgg ctgcgtaccg caggacgtct tcctcttcca gggcagcgtg
    26761 cgcgagaaca tcaccatggg tgcttccttc gccgacgacg ccgccgtgct gcgggcggcg
    26821 gagatcagcg gcgtcgagga cttcgtcagc cgccacccgc tcggctacga catgcccgtc
    26881 ggcgagcgtg gcgagctgct ctcgggcggg caacgccagg cgatcgcgtt ggcgcgagcg
    26941 ctcctgctcg acccgccgat cctgatcctg gacgagccga ccagtgctat ggacaacggg
    27001 gcggagagcc ggttcaagac gcggctcggc gaggagctcg ccgggcggac gctcctcctc
    27061 gtgacgcacc gcgcttcgct gctcggcctc gtcgaccggc tgatcgtgct ggatggcggc
    27121 cggctggtcg cggacgggcc gcgcgaccaa gtgctcaagg cgctggctgc cgggcagatc
    27181 aagggggcga actgagatgc gttcggctac tgccatcgac acccgggcgc tgcgcacgga
    27241 tccgatcgcc gcggcggcgg ccgccggcgg ctatacggtc gaggatctca ggccgaagcg
    27301 cctcagccat gtcctgctga tcgccatcgt cgcgttcctt gccgtgtttc tcgcctgggc
    27361 cagccgggcc acgctcgagg aggtgacacg cggggagggg cgggtgatct cgtcccagca
    27421 gacccaggtg gtgcagaacc tcgaaggcgg catcgttgcg gcgctgctcg ccaacgaggg
    27481 tgacattgtc gaggccggcc aggtgctgat gcggatcgac aacattcgcg ccgcctccga
    27541 ttttcgcgaa catctggccc gctcctacgc cttgcgcgcc ggactggccc ggctcgaggc
    27601 cgaggtcgcc gagacggcgc tcgagttccc gcccgacatt ctggccgagg cgcgggatat
    27661 cgccaatgcc gagacccggc tcttccagtc gcgccaggag gagctgcaga gcgagctcga
    27721 gatcctgcgt cggcaggccg agcagcgcga gcaggagctg gccgagctgc agacgcgcag
    27781 ccgccagtac gaggagtcgc tcaggctcgc ccgcgaggag ctgcggattc tgacgccgct
    27841 cgccgagcga cgggtggtca gccagtcgga gctcttgcag ctgcagcgcc aggtcaacga
    27901 tctcgacggg cagctcgggc agaccaagct cgcgatcccg cgcatcgagg cggccgttcg
    27961 cgaggcgcat cagcggatcg agaatgcctt cagcacgttc cgcagcgagg cctatcgcga
    28021 gatgagcgcc ctgcagagcg agctcgccgg catcagggag gtgatcacgg ccggcgagga
    28081 tcgggtgcgg cggaccgagg tgcgctcccc ggtgctcggc acggtcaagc agctccactt
    28141 caacaccatt ggtggcgtga tccagccggg cgatgcgatc gtcgagatca cgccgctcga
    28201 ggactcgttg ctggtcgagg cgcaagtccg cccggccgat atcgctttcc tgcgtcccga
    28261 tcttcccgcc atggtgaaga tcacggctta cgacttctcg atctatggcg ggctgaaggg
    28321 gcatgtcgag cagatcagcg ccgacaccat cgtcgacgat cgtggcgagc gattctaccg
    28381 cgtgcgggtc cgcaccgagg agaactcgct cggcaccgcc gccgaaccct tgcgcatcat
    28441 cccgggcatg accgcccagg tcgacgtgat caccggcgag aagaccgtcc tcgactatct
    28501 gctgaagccc atcctcaagg cacgcgacca cgccctgcgc gaacgctgat cgctaggccg
    28561 gcgcggcagc acgcccggct cgagagccga tccaatggct cgcgaggaca tcgacaagga
    28621 agcgggtcgt cgcgacgcct cgttgctgcg atcgatgcgg gcggcccgta ggagggttgc
    28681 ggaatcgccg gcatgaccgc gtgatctgcc gcgcccggcc gcacccggct ggcgatcagg
    28741 gaccggccca ggcaccgaaa tctggtgaag ccgttatcgg tcgaggccgt tctagccttt
    28801 tgtggatgcg ggagagccgc tccagcgtct ccgcagcggg atctgcgttc gagcttcgtg
    28861 aggtcatgcg cgagtcggag agggggccgc taatgcttgt tggcgctgtg gtcgaagcgg
    28921 gtggcgcttt gcaagcccgg cgggatctgc cgcacggcca cgtggtgtga tgcgcttcgc
    28981 agcatgccgt cctggatcgg ccccggtctt gccgttgcag ccatgcgacg ttccggcatc
    29041 ggtccggact gtgccttgcg cgacgtctgg caggatggag aagccgcgat ctcgaggaat
    29101 tccgggtcag ccatcgaacg ccgcgatgcc cccggccgac gtggtcgtca tgccgtgcag
    29161 gtccacgatc gggtctgtcg ccttcgggcg tgggggcgcg gaccggtcac gctccgggac
    29221 ggcgttcgtg acggcgcttt ttgcacgcgg catgtgtcga gctttggcaa gcggcccggc
    29281 ctactggcac gggcttccgt tcagtcggag ccgccgagat cgacgaggac gacctggcgt
    29341 ttctgctcca gcatgacgtt gaggacgatg cggcggcgct ttcggtccca gacgggatgg
    29401 gggtcgacgc gcctggcctt gaggcctggc gggccgctgt cgaccagcat gagctgcgac
    29461 tccttgccgg cggcgaggtc gacgaggcgg accggtgcgc ggccattggc gtcgccatag
    29521 ccttcgttga gataggcgtc ggtaagcagc aggcgtccgg tcggatcgag gctcgggtgg
    29581 ccgctgccgc ggaacccgcc gacgagctcg gcgaggccat tgccgtcatg accgaagcgg
    29641 acgaagcgca tctcgtggcc gccgggcggc acgaggttca tgaggatggt ctcgtcgtcc
    29701 ggcgtccagc tcgggtggtg gccgccgcgg tcccagagcc ggtgcggcag ggccaggcgc
    29761 accgcgtcgc cctcggccga gagggtgagg atcatggcgc gcaccttctc gggcgcgtcg
    29821 gcgagcccgg tcgagcgcac cacctggaag aggcggtcgc cggtgccgtt gaccttggtg
    29881 tggaagaggg aatgccggcc gctggcgagc agcgcgcgct cggggtgatc ggcaaggagg
    29941 gcgtccaccg agacgagcag ctcgcagcgc ccgctctcga gatcggtgcg ccagataccc
    30001 tcatccgtgg cgaagccggt ggtgggccgc cggcggccca agagagggtc ggggacgccg
    30061 tagccggcct gggtggcgtt gatgaggtcg agcgccggac cgaaggcgtg gcggccccgc
    30121 gggcagagcg cgtagaccgg accgcccagg ggccgggccc gccgctcgga caggtcgatc
    30181 accaccccga ggccgcggcc accgatgcgg tcgttgcagt agagcaggtc gtcgcgcccg
    30241 ggatgccagt gcagattggc gccgagctgc agcgcccagc cgcgcgtgtg gtagaccgtc
    30301 tcgatcgtgc gttcggcgag gtcgatgacg cagacttccg cggtgtcgcc cggatagggg
    30361 ccgcgatgct ggaagggcag gcgggtgacc gccagatagc ggcccgaggg gctgatcggg
    30421 gggacgtcga agaaggtgtg cagataggcg ccgtcgggcg gcgtgacgac ggtcgcgcag
    30481 gcgcccggat gcaatgtcgg gtagctgcgg aaagcgacct cgcgcctgac gaagagcggc
    30541 gtcggtgccg ccgctgcctc gagccggagg ccgcgtgcca gggcacccag ggcggtgcgc
    30601 agctcctcct cgccgttgcg ggcgatccgg cggagcgccg cgtagcggct gcgccagcgc
    30661 aaggcgggag ccggagtggc ggtcgccaga gccgtcatcg ttccgttcac ccttcatgct
    30721 tgttcatgcc gctgccgcgg tgatagccga gtcggatgaa gggtatctta agacgggccc
    30781 cgtcgcgggc actggcgtga cggtgccgga atggcgagcg ccccggccgc cgctggggcg
    30841 accggggcgc ctacgccccg tggccgcttc gaccggcgag gcggtcgaag cggcagccgg
    30901 ctcaggcgcg acgcacgtcg agggtggcga agtgctgcat gccctggcgg gcgatcagga
    30961 cgaggaccgc ctcgtgctcc tcttccgatg cggcccggac ccgctcggcc acctcggcgg
    31021 gcgtcgtcac cggtcggttg tcgaccgaga ggatgacgtc gcccggctgc accgtgccgt
    31081 cggcatcctc ggtgacaccg acgaccagca cgcccttggc gtcggcgtcg aggccggcgg
    31141 cctgacgcgt ctcgtcgttc agtgcggcga gatcgagccc gagcacccgg tccggcgccg
    31201 tctcgaccgc cgcctcggcg acatcctggg tcggcgcggt tcgcagctcg gcctgcagct
    31261 cctcgtgggc gccatgacgc cagacatcga ccgtctgcgg gacatcgatc tccgccgccg
    31321 ccaccagcct gctgagatcg cgcaccgcga cgatcggggt gccgtcgaag gcggtgacga
    31381 cgtcgccgac ctggagcccg gccgtcgcgg cggggctgtc cggcgtgacc tgggcgacca
    31441 gggcgccctc ggccttgtcg aggccgagac tctgcgcgat ctcgggggtc accggctgga
    31501 tctgcacgcc gagccagccg cggctgacga agccctgctc cttgagctcc gcgatgatcg
    31561 gctgcgccag gttggcgggg atggcgaagc cgatgccgac attgccgcca ttgggcgaat
    31621 agatggcggt gttgatgccg acgaccgtgc ggttgacgtc gaaggtgggt ccgccggaat
    31681 tgccgcggtt gatggacgca tcgagctgca ggtagtcgtc g
//
LOCUS       DAOTFI010000016        30666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1669_length_30666_cov_6.931038, whole genome shotgun sequence.
ACCESSION   DAOTFI010000016 DAOTFI010000000
VERSION     DAOTFI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1669_length_30666_cov_6.931038"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..2159)
                     /gene="aspS"
                     /locus_tag="P5558_02675"
     CDS             complement(342..2159)
                     /gene="aspS"
                     /locus_tag="P5558_02675"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRY23246.1"
                     /translation="MHRYRTHHCGELTKGWVGSEARLSGWVHRKRDHGQLLFIDLRDH
                     TGLTQIVFQPEQPFFAEAEGLRLESVITVSGAVVARSPETINPDLATGEIELVAAGLD
                     VQSVADTLPLQVNAERDYPEETRLKYRFLDLRREKMQRQIRLRSAITASIRRRMTEQG
                     FTEIQTPILTASSPEGARDFLVPSRLHPGHFYALPQAPQQYKQLLMMSGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDLEMAFVTQEDVFAAVEPLMHGLFDEFSDRPVTPMPFPH
                     IAYRDAIRRFGTDKPDLRNPIEMQDVSEAFRGSGFKVFAGILAGDPKAAVWAIPAPRG
                     GSRAFCDRMNAWAQEQGQPGLGYIFFKDGQGSGPVAKNIGPERTATIRDQLGLEDGDA
                     VFFAAGLPDKFYKFAGAARDRVGQELDLCEKDVFRFCWIVDFPMFEWDEDTRSVAFSH
                     NPFSMPQGGMEALLTRDPLDILAFQYDIVCNGVELSSGAIRNHRPDIMIKAFELAGYD
                     RQVLEDRFGGMFRALHFGAPPHGGIAPGLDRIVMLLADTENLREVIAFPMNQQAQELL
                     LGAPSPVGKKQLEELHLQLSLAARERLRELAGGPPAAAS"
     gene            2287..3438
                     /gene="rnd"
                     /locus_tag="P5558_02680"
     CDS             2287..3438
                     /gene="rnd"
                     /locus_tag="P5558_02680"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRY23247.1"
                     /translation="MTLITETSDLRAVVERLRQEPFVTIDTEFMRERTYWAKLCLVQL
                     AGEAEAVAIDTLAPGIDLAPLLDLMADIRVLKVFHACRQDLEIFLRLMDGRMPTPVFD
                     SQVAAMVCGFGEEVAYETLVNRVAKAKLDKSSRFTDWGRRPLSEAQLTYALSDVTHLR
                     TIYKKLRSRIEQAGRTEWVAEETAGLTQPGLYVTEPEDAWKRLKVRSREPRFIALVQE
                     LAAWRERKAQARDLPRNRVIRDDLLMELAAQKPKDHEALRRLDRINLDRESMREVLEV
                     ITAVHALPESALPQLPQPKPPPRGLGPTVDLLRVLLKHCAEESDVAQRLIATSAELEE
                     LALDDEADVPALKGWRRELFGARALDLKAGRIGLAVRDRQIVLIEPPAS"
     gene            3435..4232
                     /locus_tag="P5558_02685"
     CDS             3435..4232
                     /locus_tag="P5558_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23248.1"
                     /translation="MILVTGAGGLIGRHVARALGPLARASGHRALGEPGLLDGIETVI
                     HAGRDPKLGTPHYRLEDDVELALARRVAEQGLKLLTLSSRKVYAPSDRPLGEESALGP
                     TDAYGRQKLAMEEALAALLGDRLTRLRLANIFGFEAGRPSFMGRMLDGLARDATVTFD
                     MSPFTTRDFLDVDTASRMIAAIARRPPGGIVNVGSGVALPTGRLALALIEGFGRGRLV
                     VLDASERDAFVLDCRRLTRLVGPRPDAAALLDQARRLGVRLSESARP"
     gene            complement(4210..5064)
                     /gene="pdxY"
                     /locus_tag="P5558_02690"
     CDS             complement(4210..5064)
                     /gene="pdxY"
                     /locus_tag="P5558_02690"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HRY23249.1"
                     /translation="MQDGIVSIQSAVAYGHVGNSAAVFPLQRLGFEVWPVNTVLFSNH
                     TGYGTCRGHVLTVDQVREILRGIGERGAFDRCAAVLSGYLGDAELGLAVLETVAQVKV
                     RRPDALYVCDPVMGDLDDGFFVRPGIPEFFREHAVPAADIITPNQFELAFLAGREIST
                     FDDVLAAARLVRDLGPRMVVATSLKLAERPGELGVLAETGDGSWVIWTPRLDIHLNGT
                     GDAFTALILAHYLRHGEPTGALEAAASAMFALVKATLAAGRRELALVAAQAQLAEPDR
                     RFTAERIR"
     gene            5171..5809
                     /gene="pdxH"
                     /locus_tag="P5558_02695"
     CDS             5171..5809
                     /gene="pdxH"
                     /locus_tag="P5558_02695"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRY23250.1"
                     /translation="MLLDPAQLRQHYDAGALDEADADADPIRQFGRWFEAALQADLHE
                     PNAMALATVDAAGRPSSRMVLLKGFDAEGFTFFTNRESRKGQDLAANPRAALLFWWDR
                     LHRQVRIEGPVERISDAESDAYFHSRPYGSRIGAAASPQSRPVASRAVLEERYRALEA
                     EHPEHPPRPAHWGGYRVRPDRFEFWQGRPSRLHDRLAYLPDGGGWRIERLAP"
     gene            5889..6623
                     /locus_tag="P5558_02700"
     CDS             5889..6623
                     /locus_tag="P5558_02700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23251.1"
                     /translation="MSERRNVFITGASRGIGHATATYFVRQGWRAITCSREVVPEGCP
                     RDERHDHITVDLGDTANLGATVGQLDELLDGAPLHALINNAGYSPKGEAGARLGCLDG
                     DMATWQKTFAINFLAPVFFSRIVAPRLARTGGTVVNITSIAGHRVHPFAGSAYATSKA
                     ALTALTRELAADLAHLGIRVNAVCPGEINTAILSPGTAELVERIPLRRLGSPEEVAAV
                     IYYLATDQSSYITGAEIPVNGGQDVY"
     gene            complement(6636..7460)
                     /locus_tag="P5558_02705"
     CDS             complement(6636..7460)
                     /locus_tag="P5558_02705"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23252.1"
                     /translation="MARQARTTGQILGHGLLWAWTVAALVFLVAPILVIVPLSFNAGA
                     FLSFPMPGLSLRWYAELWQTDVWWSSLANSLIVGTGATAIAVVLGTAAAVGLAMARFP
                     GKDALMAVLISPLVVPLVIVAAGTYFFFIPLGLTGSLLGLTLVHAALGAPFVLITVGA
                     TLVGFDRNLTRAGASLGAAPFEVFLRITMPLIAPGMVSGALFAFITSFDELVVAMFLT
                     GPGQRTLPRQMFDGIRENMSPTILAVATILIVISILLLTTVELLRRRSLRLRGIRE"
     gene            complement(7467..8609)
                     /locus_tag="P5558_02710"
     CDS             complement(7467..8609)
                     /locus_tag="P5558_02710"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23253.1"
                     /translation="MPLLAFLLAFMVLPIGGMLRLAVSNGELRATMPATTEALRDWDG
                     EGVPPEALVATFAEELRAAREARSLGPVGQRLNNARPGDRSIVQGTARRLPDEPDGDW
                     RATLAGIDETWGEPRIWRSLQAASAPWTELYLLQALDLERDADGGIRAVGDDRAIYRS
                     ILGRTFLISLVVTLACLAIGYPLAYKLASLPPRIANPLTILVLLPFWTSLLVRSAAWI
                     VVLQREGPINEALLALGLVDEPVQLVFNRIGVYIAMTHVLLPFMVLPLLSVMRSIPPS
                     HMRAAISLGATPLEAFVRVYLPQTRAGIGAGCLLVFILAIGYYITPALVGGAADQMIS
                     YFVAFFTIQTVNWGMAAALGSILLVATLVLYLVYARLVGIDRLTLG"
     gene            complement(8708..9751)
                     /locus_tag="P5558_02715"
     CDS             complement(8708..9751)
                     /locus_tag="P5558_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23254.1"
                     /translation="MSRNLLRLAAFAGIAAMATVAQARDLTVVSWGGAYQDAQREVYF
                     KPFTAATGIALTEDSWNGGIGALRAKAEGPPSWDVVQVESDELELGCEEGIIVPLDWE
                     KLGGRDQYLPTAVHDCGVGAIVWATVLAYDAGRYAENPPSSWADFFDTETYPGTRALR
                     SGPRQALEFALMADGVPLDQIYDVLATDEGIERAFAKLDSIKDSLIFWEAGAQPPQLL
                     ASGEVVMTSAYNGRITAANQDEGRNFQIAWDAGFVYQIDSWVVLGNSDKEDEAMQLIA
                     FMSDPENQKLLPPFIPYGPTNKDAAALVDPAVLPNIPSSPQNIDHGLFFNGAFWVDRV
                     EDLNARFNAWAAQ"
     gene            complement(10095..11186)
                     /locus_tag="P5558_02720"
     CDS             complement(10095..11186)
                     /locus_tag="P5558_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23255.1"
                     /translation="MTSEQEAFVRFAEVQKSYDGETLVVKDLNLDIARGEFLTMLGPS
                     GSGKTTCLMMLAGFETATNGGIYLDGREINNVAPHKRDIGMVFQNYALFPHMTVAENL
                     AFPLSVRRMAKADIEARVKGALDMVQLGGFGSRRPAQLSGGQQQRVALARALVFEPRL
                     VLMDEPLGALDKQLRETMQYEIKHIHENLAVTVVYVTHDQSEALTMSDRVAVFNDGVV
                     QQLATPPVLYEKPENAFVAQFIGENNRFVGEVREIANGHCIVEVQGAPIRALPVNIEG
                     VGSRSTLSLRPERVVVDPPAGSFPNVFEARVLELIYLGDHIRARMAVCGNDEFIVKVP
                     NASEHPTLERGNAIRVGWQAEDCRALDPS"
     gene            11363..12082
                     /locus_tag="P5558_02725"
     CDS             11363..12082
                     /locus_tag="P5558_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HRY23256.1"
                     /translation="MTIPLFRDDAYLRDCRAVVVEADDMGVELDRSVFYPSAGGQPGD
                     TGWLVVGGERLAVTDTQKGAGPDTIRHLLGRPTGLTAGMEVHAVLDWERRHRLMRMHT
                     CLHLLCRAVDGVVTGGQIGDGKGRLDFDLPEAPDKEALAELLNAWIAEDRPVSERWID
                     ADELDARPELVRTLSVKPPRGDGRVRLVEIEGVDLQACGGTHVRRTGEIGPVRIGKIE
                     KKGRQNRRVQVVLGDAGDAGG"
     gene            12150..13595
                     /locus_tag="P5558_02730"
     CDS             12150..13595
                     /locus_tag="P5558_02730"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRY23257.1"
                     /translation="MAESYHTDLYIDGAWHPAMAGGHIDILDPATEEVVATAAQAERA
                     DVEAAIASAHQAFQTWRKVLAWDRSKVLRKAAMLLEERADRIARLLTLECGKPLGQAR
                     AEVISAVELIDWYADEARRIFGQTLPGRGADNRFMTIFEPVGVVGAFTAWNFPVVLQA
                     RKIAPALATGCTIVTRPAEEGTSSVAELVKCFIDAGAPKGTVNLVTGKPEVISEAIMD
                     SDLVRKVTFTGSIPVGQHLMRRAADTVKRMSMELGGHAPVIIHEDVDPVKAALASAAG
                     KSRNNGQVCVSPTRFYVHKKHERAFTDAFAGALAGMKIGHGLEPGTEIGPLVNRKRLA
                     FVEEMVDQTRAEGARVVTGGQRPADMNRGFFFEPTVFSDVRDDMRVMNDEPFGPIAPV
                     TTFETFDEVIGRANSLSYGLAGYVFTGNQKLAQATAEALRTGIVGVNTFAAATAEMPF
                     GGVNHSGFGRENGSQGLYDYLDAKFVNIAYG"
     gene            13609..14724
                     /locus_tag="P5558_02735"
     CDS             13609..14724
                     /locus_tag="P5558_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY23258.1"
                     /translation="MTEPRPRLTDLVQGLPATIPFVGPEALERRRGRLFELRLGANES
                     AFGLSPLAAEAIRRELARAAWYGDPEAFELRERLAGLHRVGLDEIVVGAGIDELLGVL
                     VRAVTGPGDPVVTSAGAYPTFNYHVAGFGGRLVAVPYRDDHEDLEGLALAVRREGAKL
                     VYLANPDNPMGTWHTGSAVQGFIAGLPADVLVLLDEAYVELAPADTAPPIDTADPRVV
                     RLRTFSKAHGMAGLRIGYAIAHAEVIQAMGKIRNHFGVSRVAQAAAMASLDDAAFVAG
                     VVAEVARGRDEYAGLAAGLGFRALPSATNFVAIDVDPEKGGPERGRALLEALADAGVF
                     IRRPGIAPLDRCIRVSVGTAPERARFADIFSRIVRGL"
     gene            complement(15283..15678)
                     /locus_tag="P5558_02740"
     CDS             complement(15283..15678)
                     /locus_tag="P5558_02740"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRY23259.1"
                     /translation="MRKLMLAMTVAALPAAALAAEVDGVWKSEANEDGAYIEITMAPC
                     AADASLTCGTISKAVTKDGDDPTYANLGKPIVENMKAEGDGNYSDGTIWDPESDKTYK
                     SNMTLKGDTLNVEGCVLFICEGQDWTRVQ"
     gene            complement(15839..17023)
                     /locus_tag="P5558_02745"
     CDS             complement(15839..17023)
                     /locus_tag="P5558_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY23260.1"
                     /translation="MRLPKEPERSGVSGRSAIEPFLVMQVLAAANKRAAEGGDVLHLE
                     VGEPSGGAPRAALDAARAALDGSALGYSEAFGLRPLRTAIARHIEATYARAVPIERIA
                     ITAGASGGFILAFLAAFDAGARVAVAEPGYPAYRNILKALDIEVVKLVTSETSGFQPT
                     VADLEAIGGPLDGVIVASPANPTGSLIDPAELAAIARWCDARGVRLISDEIYHGITYG
                     RPGSTALASSTRAIVVNSFSKYWAMTGWRIGWLVLPDELVDPVERLAQNLFISPSAVG
                     QHAAIGALGAKPELDARVAGYGENRALLLDALPRIGLDRLVPPDGAFYLYADIGRFGL
                     DSVDFCARLLADTGVAITPGNDFDVARGNHYVRLSFAGARTTIEQAIDRLGPWLARLD
                     RR"
     gene            17233..18990
                     /locus_tag="P5558_02750"
     CDS             17233..18990
                     /locus_tag="P5558_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY23261.1"
                     /translation="MADEQTRRRLAAILAVDVVGYSRLMATDEAGTLAALKAHRRAVL
                     EPAIALRGGRIVKLMGDGVLVEFSSVVDAVACALAIQTADQGGESAGAGALTLRVGIN
                     LGDVIIDGDDIYGDGVNVASRLEALAEPGGICISSIVHESLGGRVEAGFVDGGKVQVK
                     NIDRPIGVWRWQAGSAGAARAARVVPPAAATKASVAVLPFDNMSGDPEQEYFSDGISE
                     DIITDLSKIGGLVVIARNSSFAYKGRRVDIREVARDLGVGTVLEGSIRRAGNRVRINA
                     QLIEAATGAHLWAERYDRELTDIFAVQDDVTRHIVDALKVELTRGEEARIAAVPTMSH
                     EAHDLFLRGREHIRRGVWTRAILEEAVRTFGQAIALDADYADAHAGLALCHSIDFQNR
                     LTGDPTALATAGRIVEVAIAKGPDLPFPHWAAAAIVIWQKDLARARRECEAALALSPN
                     YALAHATRGHAEIYAGTPLKAIPYLERAIRLDPAFTQQYLHFIGSAYLAAGRYEVAAA
                     TFRERIRLAPETDISRGLLIAALGHLGLVEEAPEVWDELKRVNPGYSFEAHLARLPFS
                     DPADAARIAAGFARAGVAG"
     gene            complement(19099..19785)
                     /locus_tag="P5558_02755"
     CDS             complement(19099..19785)
                     /locus_tag="P5558_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY23262.1"
                     /translation="MAPHVPHHVTQRGNRRQTVFFADADYRLYRDLLRTHCRAARVAV
                     WAWCLMPNHVHLVVVPDAPDSLARAIGDTHRDYTRAVNRRHGWTGYLWQGRFGSCAMD
                     EPHCLAAIRYVERNPERAGLVADAAAWPWSSARAHLGLGADGLVEPHGLDHLVPDWRA
                     FLDDTPPSWQLDELRRHSRSGRPLGSTAFLERLEAALGRKLRHARLGRPPGTNESPAD
                     RAPQTGESDR"
     gene            complement(19920..20615)
                     /locus_tag="P5558_02760"
     CDS             complement(19920..20615)
                     /locus_tag="P5558_02760"
                     /inference="COORDINATES: protein motif:HMM:NF018597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HRY23263.1"
                     /translation="MAAQDGDQGAGPADRRDRAGAGWLTRNVALVALAGLLLGACAGP
                     PPSRQAATRWPSDPAACRALLATEGVRFTPWAGAPADGACAVPAPVVVTWTSAAMAPT
                     LRTSCPMLLAWARFEPEMQRLADRRLGSRITEIRHYGSYSCRKMTGNQRRMSLHSTGE
                     ALDIAGFTTADGRQISVADDWPRRSGGASLFLHDLARAACRHFNVVLTPNHDRAHRDH
                     IHVDIGPWKLCKA"
     gene            complement(20543..22720)
                     /locus_tag="P5558_02765"
     CDS             complement(20543..22720)
                     /locus_tag="P5558_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRY23264.1"
                     /translation="MANGTDGELIIARDVDDEPASGNQRSLTGFSHHFVFWYCVAYTS
                     WHLLVLNVFPLETWAFRTLHVAGGLGIGFLVATHAVPRLERPGGGLPEQLLRPLRLVA
                     AVLVAVALLAMLAPFGLRELAGLPNPPPWVYAIGAPALLAGTGLAMLLAWLTPADTSR
                     IDPADWTMAAAAVAVALYLLFNLQGLQFRAGVMPTRPDFWVTLAGVALILECTRRLAG
                     LALVVISMAFILYAFVGPWLPGFLAHSGYSADRFFTYLYTDNGILGPTTAVSSTYIIL
                     FICFAAFLQASKVGDYFVNFAFAAAGHARGGPAKVAVFASGLMGMINGTSAGNVVATG
                     SLTIPLMKRVGYRARSAAGIEAAASTGGQIMPPIMGAGAFIMAEVTGIRYTEIAVAAL
                     IPATLYFISIYFMVDLEAQRTGMKGLPRDQLPRFDVLAKQAYLFVPIVILIAALFMGY
                     SVIRAGTLSLVAAVVVSWLTPHRLGPVGIVKALNLTATMAVQLIAVCACAGIIVGVIG
                     LTGVGLRFSAMLLTVADTSQFLALLFAMGISLMLGMGMPTTAAYAVAASVVAPGLIRL
                     GIEPLIAHFFVFYFAVISAITPPVALAAYAGAAIAGSRALETSLTAFKLGLAAFIVPF
                     MFFYSPALLMMDTWPAIIQSLATACVGVFMLSAAVQGWFFGRTSILLRLVIGAAALLM
                     IEGGLVTDLVGIGLGGAVFLWQRKMAIKEPDLPIAETAREPVG"
     gene            complement(22785..23786)
                     /locus_tag="P5558_02770"
     CDS             complement(22785..23786)
                     /locus_tag="P5558_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY23265.1"
                     /translation="MMRTCLTGLGMTALAGVLALAPATGTAQDKTGWPSSIKVGTASQ
                     GGTYFIYGAGWAGLIQEMLGVPASSEVTGGPVQNFALTQTNDVQMAMTTMGPAREAWD
                     GLSPIAPGVQMRDVRATFPMYQTPFQIIALESSGIQSVADLDGKTVGNGPRGGTCGTY
                     FPQYFDALGVETNDQFGGASDLAGQVKDGLIDAFAFCAGLPIAAFSELEAQNPVNIFA
                     FTPEEQATLVEQFPVSAFEIPADTYTSQHEPQHSVAMWNFGIAHKDLPQSFVYEVMKV
                     VLDNNDRMMQIHQAAAETLPENWVHNKFLPFHPGAVQYYKEKGFDIPENLIPAEYEG"
     gene            complement(23905..24423)
                     /locus_tag="P5558_02775"
     CDS             complement(23905..24423)
                     /locus_tag="P5558_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY23266.1"
                     /translation="MPRFPAALALLLGLAAPAAALEPVAGEPVPVPPVEIRDMAGQSF
                     DLAAAVATGEVVVLNFWATWCAPCKREMPTLAALQAAFEGRPLRVVTLAMDRAGPEAL
                     QAFMTDIGVDDLTIWRDPQMATSHPLGIPGLPVTLVIDGQGNEIFRHAGYADWAAPEI
                     VAFLDGLLLNMP"
     gene            24475..25878
                     /gene="argH"
                     /locus_tag="P5558_02780"
     CDS             24475..25878
                     /gene="argH"
                     /locus_tag="P5558_02780"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRY23267.1"
                     /translation="MTRDTKAARDLWGGHFASGPAPLMEKINASIGFDKRLWPQDVRG
                     SMAHARMLAERGIIAPADRDAIIEGLEALEAALEEGSFVFDAKLEDIHLNIEARLTER
                     IGEPGRRLHTGRSRNDQVATDFKLFVRDAMDRAVALLKGLVGVLADRAEAEAATVMPG
                     FTHLQAAQPVTFGHHLLAYGEMFMRDVSRFSDARRRLNECPLGAAALAGTSFPIDRAQ
                     TAAALGFVRPAANSIDAVSDRDFALDYLASAAIAAIHLSRLAEELVLWSTPQFGFVKM
                     AEAFTSGSSIMPQKRNPDAAELVRGKSGRVIGHLQALLVTLKGLPLAYSKDMQEDKEG
                     VFDAVDTLELCLEATAAMLASLTVDPARMRAAAGVGYTTATDLADWLVRVKGAAFRDA
                     HGSAARAVKAAEERGCGLHELDADTLRAIDPRLEPAALLDVDASVASRTSFGGTAPER
                     VREAVTALRGRLAELAR"
     gene            25875..26045
                     /locus_tag="P5558_02785"
     CDS             25875..26045
                     /locus_tag="P5558_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HRY23268.1"
                     /translation="MRRRALLGLVAVGVLAACGRRGPLRLPDAPPLEQPPVDNSIRDI
                     PEPEPTDDGTAG"
     gene            26042..27322
                     /gene="lysA"
                     /locus_tag="P5558_02790"
     CDS             26042..27322
                     /gene="lysA"
                     /locus_tag="P5558_02790"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRY23269.1"
                     /translation="MRAFTYRDGGLACEGVPLEAIARAVGTPAYVYSAGAMRARLREF
                     QDAFAARPALFCYAVKANHNLAVIRTLANAGAGADTVSGGEIERALAAGVRPERIVFA
                     GVAKTDDEIRFALRTGVLQLNVESVPELLRIDAVAQGLGLQAPVAFRINPDVDAGTLS
                     KISTGRKGDKFGVPFDEAADLYALAASLPGIRPEGLHLHIGSQITRLDPFAAAYRRGI
                     ELFRTIRAAGIPLRRLDLGGGFGVRYRNEEPVPASAFAALVQGLTAGLDVELVFEPGR
                     ALVAEAGALVASVIYRKESGGRRFLILDAGMNVLIRPALYDAWHDVLPLAEPPPKAHL
                     EPMDVVGPICESSDIFGRDRYLPLLDAGDRVAFLSAGAYGSVMSSDYNSRVSAAEVLV
                     DGDRWAVVKPGREPSRQFADETIPDWLAAGSEGR"
     gene            27364..29838
                     /locus_tag="P5558_02795"
     CDS             27364..29838
                     /locus_tag="P5558_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HRY23270.1"
                     /translation="MAEGALPMSSRSFRRKLGLARLAVSFERLYPALFPALLVVAGFL
                     ALGLFDFWRMVPVWLHVAGLLVFALALGWSIYRVRDRFRLASRDAGLARLERDSAARH
                     QPLRALEDRLADDWQDPAGQALWRRHRERVLAALGRVRLAPPRSALPARDPWAVRALV
                     LLLLVVAVVDARTDAGRRLVRAFVPGTVGAAALPIEVSLWVTPPHYTGRPPLTIEQTR
                     RLERLDVPIGSEAVLQLHNLPAGDGGRPEVLFNGEALAQEALGERSGEVRLDLVEAGQ
                     LEVLKADGEPLAGWQVAVLVDHPPVIAFAEPPRATHRSVLELAYAASDDYGIGLVALE
                     LALVERPEERDRAVLAEPARAPTEVASTTYLDLTAHPLAGLEVALNLVAEDGLGQVGT
                     SETVVMVLPERVFEHPLARAVIAERRRLVREPGARRAVTAALGELAESRLAEQAGTAV
                     QLGLAVAQARLRGARDAAERRSVVDLLWELALMIEDGGLSMAERSLREIQEALQRALE
                     EGASEAELEALMDELREAMDRYLEELTRQALEQMRNMPPDMRMQPIDPSQMVDRQSLQ
                     EMIDRAEEMMQSGAREQAQAMLEQLQQMLENLQAGVPQMQPHPGEQALSDLQRMIELQ
                     QNLLDRSYQMQQGQPGQQSEPGQQGQQGQEGQEGQQGSGQAAAEQDALRRALGELMRR
                     LGEAGMDIPRALGQAELQMRGARDALQEGQPGRAVGPQGDAVDLMQQGGQAMLDELRE
                     QMANQPGGGQGMAPGQMGRQREGRDPLGRASRNEGGWDSEGTEIPAENDLGRARDVLE
                     ELYRRSRDQGRPQQELDYFDRLLERF"
     gene            29898..30548
                     /locus_tag="P5558_02800"
     CDS             29898..30548
                     /locus_tag="P5558_02800"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY23271.1"
                     /translation="MALLVLHYTGMRSAAAAIARLTDPAAKVSAHYVIDTDGTVLELV
                     PEACRAWHAGVAGWGAIRDVNGQSIGIELVNPGHDWGYRPFPAAQIAALVELGQAIRA
                     RHGLPPKAVVGHSDVAPARKIDPGELFPWADLARHGLGIWPAAWENEAPDTGAALEAL
                     RAIGYRPDLPDTTPARVIAAFQRHWRPDRIDGRLDEATMGLIAAVHRLSKAAPDAT"
BASE COUNT         4632 a        10268 c        11327 g         4439 t
ORIGIN      
        1 tacgcgacag tgcggctcgg gttgtcgcag attccgcgac gtttgcgctc gggttgtcgc
       61 ggattccgcg acatccgggc gccagatgtc gcagattccg cgacatgaat ccgtgagtgt
      121 cgcggaatcc gcgacagccg acgaccaacg aacccgcgga atcgacgatc gaacccggga
      181 aaacgccttg aatcgacgaa cgaacccagg gattccgacg aacgaaccca aggaagtccc
      241 gcggatttgc tgcctatccg ggttcctgtg ccccaagaaa gcgtgttttg ccgaaggaac
      301 ccgcccgcgc ttgacggggc gccgaaacta ggttataaat cctatgacgc tgctgccggc
      361 gggcctccgg ccagctcgcg caggcgctcg cgggcggcga gcgagagctg caggtgcagc
      421 tcctcgagct gcttcttgcc aaccggcgag ggggcgccga ggagcagctc ctgggcctgc
      481 tggttcatcg ggaaggcgat gacctcgcgc aggttctcgg tgtcggccag gagcatgacg
      541 atgcggtcga gcccgggggc gatgccgcca tgcggcgggg cgccgaagtg cagggcgcgg
      601 aacatgccgc cgaaccggtc ctcgagcacc tgccggtcgt agccggcgag ctcgaaggcc
      661 ttgatcatga tgtccgggcg atggttccgg atggcacccg aggagagctc cacgccgttg
      721 cagacgatgt cgtactggaa ggccaggata tcgagcggat ccctggtcag gagcgcctcc
      781 atcccgccct gcggcatgga gaaggggttg tgcgagaagg cgacgctcct ggtgtcctcg
      841 tcccactcga acatggggaa gtcgacgatc cagcagaacc ggaagacgtc cttctcgcag
      901 aggtcgagct cctggccgac ccggtcgcgg gccgccccgg cgaacttgta gaacttgtcc
      961 ggcaggccgg ctgcgaagaa gaccgcatcg ccgtcctcga gcccgagctg gtcacggatc
     1021 gttgccgtgc gctccggccc gatgttcttg gcgaccgggc ccgagccctg gccgtccttg
     1081 aagaagatgt agccgagccc gggctggccc tgctcctgcg cccaggcgtt catccggtcg
     1141 cagaaggccc ggctgccgcc cctgggcgcc gggatcgccc agacggcggc cttggggtcg
     1201 cccgccagga tgccggcgaa gaccttgaag ccggagcccc ggaacgcctc gctgacgtcc
     1261 tgcatctcga tcgggttccg aagatccggc ttgtcggtgc cgaagcggcg gatcgcgtcc
     1321 cgataggcga tgtgcgggaa gggcatgggt gtcacgggcc ggtccgagaa ctcgtcgaag
     1381 aggccgtgca tcaggggctc gaccgcggcg aagacgtcct cctgcgtgac gaacgccatc
     1441 tccaggtcga gctggtagaa ctcgcccggg ctccggtcgg cgcgcgcatc ctcgtccctg
     1501 aagcagggcg cgatctggaa gtagcgatcg aagcccgaca tcatgagaag ctgcttgtac
     1561 tgctgcggcg cctgcggcag cgcgtagaaa tgccccgggt gcagcctgga cggcacgagg
     1621 aagtcgcgtg cgccctcggg cgagctggcg gtcaggatgg gggtctggat ctcggtgaag
     1681 ccctgctcgg tcatccgccg gcggatgctc gcggtgatcg ccgagcgcag ccggatctgc
     1741 cgctgcatct tctcgcgccg gaggtcgagg aagcggtact tgagccgggt ctcctcggga
     1801 tagtcgcgct cggcattgac ctggagcggc agggtgtcgg cgaccgactg aacgtcgagg
     1861 cctgccgcca ccagctcgat ctcgccggtg gcgaggtccg ggttgatggt ctccggcgag
     1921 cgggccacga cagcgcccga gacggtgatg acgctctcca gcctgagccc ctcggcctcg
     1981 gcgaagaagg gctgctccgg ctggaagacg atctgggtca gcccggtgtg gtcgcgcagg
     2041 tcgatgaaca gcaactggcc gtgatcgcgc tttcggtgta cccagccact caaccgggcc
     2101 tcgctgccga cccagccttt ggtcagctcg ccgcagtgat gcgttcgata acggtgcatg
     2161 ccgccactct cgacttgtaa ggaaaggaag gctgcgggct cgcggccgcg cgtaaaggtc
     2221 acaacgctcg ccgacctgtc aagcggcgag ccgaacccgg cgcgagcagc ccgcctccga
     2281 tcgcccatga ccctgatcac cgaaacgagc gacctccgag ccgtcgtcga acgcctgcgc
     2341 caggagccgt tcgtcacgat cgatacggag ttcatgcgcg agcgcaccta ctgggcgaag
     2401 ctctgcctcg tccagctcgc gggcgaggcg gaagcggtcg ccatcgacac gctcgccccc
     2461 ggcatcgacc tcgcgccgct gctcgacctc atggcggaca ttcgggtgct caaggtcttc
     2521 cacgcctgcc ggcaggatct cgagatcttc cttcgtctga tggatggccg gatgccgacc
     2581 ccggtcttcg acagccaggt cgcggccatg gtctgcggct tcggcgagga ggtggcctac
     2641 gagacgctgg tcaaccgcgt ggccaaggcc aagctcgaca agagctcgcg cttcaccgac
     2701 tggggccgcc gcccgctctc cgaggcccag ctgacttatg cgctttcgga cgtcacccat
     2761 ctccggacga tctacaagaa gctccggagc cggatcgagc aggccggccg gaccgagtgg
     2821 gtggccgagg agacggcggg cctgacccag cccggcctct atgtcaccga gcccgaggat
     2881 gcctggaagc gcctcaaggt gcgctcgcgc gaaccgcgct tcatcgccct cgtccaggag
     2941 ctcgccgcct ggcgcgagcg caaggcgcag gcccgcgacc tgccgcgcaa ccgggtgatc
     3001 cgcgacgatc tcctgatgga gctggcggcc cagaagccca aggatcacga ggcgctgcgc
     3061 cggctcgacc ggatcaacct cgaccgcgag agcatgcgcg aggtgctcga ggtgatcact
     3121 gccgttcacg ccctgcccga gagtgctttg ccgcagctgc cgcagccgaa gccgccgccc
     3181 cggggcctcg gtccgacggt ggatctcttg cgggtgctgc tcaagcattg cgccgaggaa
     3241 agcgacgtgg cccagcggct gatcgccacc agcgccgagc tcgaggagct ggcactggac
     3301 gacgaggccg acgtccccgc cctcaagggc tggcgccgcg agctcttcgg tgcacgcgcc
     3361 ctcgacctca aggccggccg gatcggcctc gccgtcaggg accgacagat cgtcctgatc
     3421 gagccgcccg cttcatgatc ctcgtcaccg gcgccggcgg cctgatcggt cgccatgtgg
     3481 cccgggcgct cggcccgctg gcccgggcca gcggtcacag ggcgctcggc gagccgggcc
     3541 tgctcgacgg catcgagacg gtgatccatg ccggccggga cccgaagctc ggcacgccgc
     3601 actatcgcct ggaggacgat gtcgagctgg ccctggcgcg acgggtcgcc gagcagggcc
     3661 tgaagctgct gacgctttcc tcgcgcaagg tctacgcccc gagcgaccgg cccctgggcg
     3721 aggagtccgc actcggcccg accgacgctt acgggcggca gaagctcgcc atggaggagg
     3781 cgctggcagc gctcctcggc gaccgcctca cccgcttgcg cctcgccaac atcttcggct
     3841 tcgaggccgg ccggccgagc ttcatgggcc gcatgctgga cggcctcgcc cgtgacgcca
     3901 ccgtcacctt cgacatgagc ccgttcacca cccgggattt cctcgacgtc gacacggcga
     3961 gtcggatgat cgccgccatc gcccgccggc caccgggcgg catcgtcaat gtcggctccg
     4021 gcgtggcgct ccccaccggc cggctggcgc tggcgctcat cgaaggcttc ggccggggcc
     4081 ggctggtcgt cctcgatgcc agcgagcgcg acgctttcgt gctcgattgc cgcaggctca
     4141 caaggctggt cggcccccgc cccgatgcgg ccgccctcct cgatcaggcc cgccggctcg
     4201 gcgtccggct cagcgaatcc gctcggccgt gaaccggcgg tccggctccg ccagttgcgc
     4261 ctgggccgcg accagcgcca gctcgcggcg cccggccgcc agggtcgcct tgaccagcgc
     4321 gaacatggcg ctcgccgccg cctcgagggc gccggtcggc tcgccatgcc gcagatagtg
     4381 ggcgaggatc agcgccgtga aggcgtcccc ggtgccattg agatgaatgt cgagcctcgg
     4441 agtccagatc acccagctcc cgtccccggt ttcggccagc acgccgagct cgcccggccg
     4501 ctcggcgagc ttgaggctgg tcgccaccac catgcgcggc ccgagatcgc ggacgagacg
     4561 ggccgcggcc aggacatcgt caaaggtcga tatctcccga ccggcgagga acgcgagctc
     4621 gaactggttg ggcgtgatga tgtcggcggc cggcacggcg tgttccctga agaactcggg
     4681 aatccccggg cgcacgaaga agccgtcatc gagatcgccc atgaccggat cgcaaacgta
     4741 gagcgcgtcg ggtcggcgaa ccttcacctg cgccaccgtc tcgagcaccg cgaggccgag
     4801 ctcggcatcg cccagatagc cggaaagcac ggcggcgcag cgatcgaagg cacctcgctc
     4861 cccgatcccg cgcaggatct cccgcacctg atccacggtc aggacatggc cgcgacaggt
     4921 gccgtagccg gtgtggttgc tgaacagcac ggtgttgacc ggccagacct cgaagccgag
     4981 ccgctgcaac gggaacaccg cggcgctgtt gccgacatgg ccataggcca cggccgattg
     5041 gatcgaaacg atgccatcct gcaaattcga gcctccgggc ttccaccgat ccggtgaagc
     5101 gcacaataat gtccccggcc agcaccacgg cggcggctgg ttgcggagcc actttcccag
     5161 gagaacgccg atgctgctcg atccggccca gttgcgccag cattacgatg ccggcgccct
     5221 cgacgaggcc gatgccgatg cggaccccat tcgccagttc ggccggtggt tcgaggcagc
     5281 gctccaggcg gacctccacg agccgaacgc catggcgctc gccacggtcg acgcggccgg
     5341 ccggccgtcg tcccgcatgg tgctgctcaa gggcttcgat gcagagggtt tcaccttctt
     5401 caccaaccgc gagagccgca agggccagga cctcgccgcc aatccccgtg cggccctgct
     5461 cttctggtgg gaccggctgc atcggcaggt gcgcatcgag ggcccggtgg agcgcatctc
     5521 cgatgccgaa tcggatgcgt atttccactc gcgcccctat ggcagccgga tcggtgcggc
     5581 ggcctcgccg cagagccggc cggtggcgtc gcgggccgtg ctcgaggagc gctatcgagc
     5641 gctcgaggcc gagcatcccg agcatccgcc acgcccggcg cattggggcg gctatcgggt
     5701 ccggccggac cgtttcgagt tctggcaggg ccgccccagc cgcctgcacg accggctggc
     5761 ctatcttccc gacggcggcg gctggcggat cgagcggctg gcgccgtagc agtctggcgc
     5821 gagaacaggc acagcccgcc cgaaccttgt gctagagacg agcggctggc aagcatggaa
     5881 ggcagggcat gagcgagcgc aggaacgtct tcatcacggg cgccagccgc ggcatcggtc
     5941 atgccacggc gacctacttc gttcgccagg gctggcgggc gatcacctgc tcgcgcgagg
     6001 tggtgccgga aggctgcccg cgcgacgagc ggcacgacca tatcaccgtc gatctcggcg
     6061 acacggccaa tctgggtgcc acggtcggcc agctcgacga gctgctcgac ggcgcaccgc
     6121 tgcatgcgct gatcaacaat gccggctact cccccaaggg agaggccggg gcgcgcctcg
     6181 gctgcctcga cggcgacatg gccacctggc agaagacctt cgccatcaac ttcctggccc
     6241 ccgtcttctt cagccggatc gtggctccca ggctcgcgcg cacgggcggc accgtggtca
     6301 acatcacctc gatcgccggc caccgcgtcc accccttcgc cggcagcgcc tacgccacct
     6361 cgaaggcggc gctcaccgcc ctgacccgcg agctcgccgc cgatctcgcg catctcggca
     6421 tccgggtgaa cgccgtctgt cccggcgaga tcaacacggc cattctctcg cccggcaccg
     6481 cggaattggt cgagcgcatc ccgcttcgcc gcctgggctc gcccgaggag gtggcggcgg
     6541 tgatctacta cctcgccacc gaccagtcct cctacatcac cggcgccgag atcccggtca
     6601 atggcggcca ggacgtgtat tgaccgcttg cccgcctatt ccctgatccc cctgagccgc
     6661 aggctccttc ggcgcagcag ctcgaccgtc gtcaggagca ggatcgagat cacgatcagg
     6721 atggtcgcca cggcgaggat ggtcgggctc atgttctcgc ggatgccgtc gaacatctga
     6781 cggggcaggg tgcgctggcc ggggccggtc aggaacatcg cgaccaccag ctcgtcgaaa
     6841 gaggtgatga aggcgaagag agcgcccgag accatgccag gtgcgatcag cggcatggtg
     6901 atccgcagga agacctcgaa cggcgcggcg ccgaggctgg ccccggcacg ggtgaggttg
     6961 cggtcgaacc cgacgagtgt cgcgccgacg gtgatcagaa cgaagggggc accgagggcg
     7021 gcatggacga gcgtcaggcc gagcaggctg ccggtcaggc cgagcgggat gaagaagaaa
     7081 tacgtgccgg ccgcgacgat gacgagcggc accacgagcg gcgagatgag caccgccatg
     7141 agcgcatcct tgccgggaaa gcgcgccatg gcgaggccga ccgcggcggc ggtgccgagg
     7201 acgacggcga tcgctgtggc gcccgtgccg acgatcaggc tgttggcgag cgagctccac
     7261 cagacatcgg tctgccagag ctcggcatac cagcgcagcg acaggcccgg catcggaaag
     7321 gacaggaacg cacccgcgtt gaaggacagc gggacaatga cgaggatggg tgccaccagg
     7381 aagaccagcg ctgccaccgt ccaggcccag agcagcccgt ggccgagaat ctggccggtg
     7441 gtgcgtgcct gccgcgccat gcgccgtcag cccagcgtca gccggtcgat gccgaccagc
     7501 cgcgcgtaga ccaggtagag cacgagcgtg gcgaccagca ggatgctgcc gagtgcggcg
     7561 gccatgcccc agttgaccgt ctggatcgtg aagaaggcga cgaagtagct gatcatctgg
     7621 tcggccgccc cgccgaccag cgccggggtg atgtagtagc cgatggcgag gatgaagacg
     7681 agcaggcagc cggcgccgat gccggctcgc gtctggggca ggtagacccg gacgaacgcc
     7741 tcgagcggcg tcgcaccgag cgagatggca gcccgcatgt gcgagggcgg tatggagcgc
     7801 atgacgctca ggagcggcag caccatgaac ggcagcagca catgtgtcat ggcgatgtag
     7861 acgccgatcc ggttgaagac gagctgcacc ggctcgtcga cgagccccag ggcaagcagc
     7921 gcctcgttga tcggcccctc gcgctgcagc acgacgatcc aggcggccga gcggaccagg
     7981 agcgacgtcc agaacggcag caggacgaga atcgtcaagg gattggcgat gcgcggcggc
     8041 agcgacgcca gcttgtaggc caggggatag ccgatggcga ggcaggcgag ggtgacgacg
     8101 aggctgatca ggaaggtgcg gccgaggatg ctgcggtaga tggcgcggtc gtcgccgacc
     8161 gccctgatcc cgccatccgc atcacgctcg agatcgagcg cctgcagcag gtagagttcg
     8221 gtccaggggg ccgaagccgc ctgcagcgag cgccagattc ggggctcccc ccaggtctcg
     8281 tcgatgccgg cgagcgttgc acgccagtcg ccgtccggct cgtccggcag gcgccgggcc
     8341 gtgccctgca cgatgctgcg atcgccgggt cgcgcgttgt tgagccgttg gccgacgggc
     8401 ccgaggctcc gcgcctcacg cgccgcccga agctcttcgg cgaaggtggc gaccagcgcc
     8461 tctggcggca caccctcgcc gtcccagtcg cgcagcgcct ccgtcgtcgc cggcatggtc
     8521 gccctgagct caccgttcga gacggcgagc cgcaacatgc ccccgatcgg caggaccatg
     8581 aaggccagga gaaacgccag aagcggcaag gtgagcccga aggcccagag ctgctgccgc
     8641 cgctcgaccc gccggagcgc ccgccggagc gccccggcat cgttgccgag ctgcgttccg
     8701 gcgctggcta ttgagcggcc caggcgttga accgcgcgtt caggtcctcg acccgatcga
     8761 cccagaaggc accgttgaag aagagcccat ggtcgatgtt ctggggcgag gaagggatgt
     8821 tgggcaggac ggccggatcg accagggccg cggcgtcctt gttggtcggg ccatagggga
     8881 tgaagggtgg cagcagcttc tggttctcgg ggtcgctcat gaaggcgatg agctgcatcg
     8941 cctcgtcctc tttgtcggag ttgccgagca ccacccaact gtcgatctgg tagacgaatc
     9001 ccgcatccca ggcgatctgg aagttgcggc cctcgtcctg gttggcggcg gtgatccggc
     9061 cgttgtaggc gctggtcatc accacctcgc ccgaggccag gagctggggc ggctgggcgc
     9121 ccgcctccca gaagatcagg ctgtctttga tgctgtcgag cttggcgaag gcccgctcga
     9181 tgccctcgtc ggtggccagg acatcgtaga tctggtcgag cggcacgccg tcggccatga
     9241 gcgcgaactc gagcgcctgg cgcgggccgc tgcggagcgc gcgcgtcccg gggtaggtct
     9301 cggtatcgaa gaagtccgcc cagctcgaag gcgggttctc ggcatagcgg ccggcgtcgt
     9361 aggcgagcac ggtggcccag acgatcgcac ccacgccgca atcgtgcacg gcggtcggca
     9421 ggtactggtc gcggccgccc agcttctccc agtccagcgg cacgatgatg ccttcctcgc
     9481 agcccagctc gagctcgtcg gattcgacct gcaccacgtc ccagctcggc ggaccctcgg
     9541 ccttggcgcg caaggcgccg atgccgccgt tccagctatc ctccgtcagc gctatgccgg
     9601 tcgccgccgt gaatggcttg aaatagacct cgcgctgcgc atcctggtag gcgccgcccc
     9661 acgacacgac cgtgagatcc cgggcctggg ccacggtggc catggccgcg atgccggcga
     9721 acgccgccag cctgagcaga tttctcgaca tgttcccctc ccttggcttc gactgtgcgg
     9781 gcaatcctgc cgcaattccg tccccggcgc actagtcctt gatgccgcgc ggccgctcga
     9841 ctcagcggcg catccaggtg ctgtagcgct cgcggtagtc gtcaccccga gcggcccaga
     9901 acagggcgtc gagcgcgatc gaggcggccg gcgccgggct ctctcgggcg tggccacaag
     9961 ggccgagccc ggcccggtcg gcgaggtgcc gggagcctaa ccggccgcgc tctcggcctt
    10021 gcggacctcg acttgcggat gcggcggcgt gcagggatcg cgataagcgg gctgccggac
    10081 ctcgacctgg cgcatcagct cgggtcgagg gcgcggcaat cctcggcctg ccagcccacc
    10141 cttatggcgt tgccgcgctc gagcgtcggg tgctcgctgg cgttgggcac cttgacgatg
    10201 aactcgtcat tgccgcagac ggccatgcgc gcgcgaatat ggtcgccgag atagatcagc
    10261 tccaggaccc gtgcctcgaa gacgttgggg aagctgccgg cgggggggtc gaccacgacg
    10321 cgctccgggc ggagcgacag ggtcgagcgg ctgcccacac cctcgatgtt cacgggcagg
    10381 gcgcggatcg gggcaccctg cacctcgacg atgcagtggc cattggcgat ctcgcgcacc
    10441 tcgccgacga agcggttgtt ctcgccgatg aactgggcga cgaaggcgtt ctccggcttc
    10501 tcgtagagca cgggcggcgt cgccagctgc tgcacgacgc cgtcgttgaa gacggcgacg
    10561 cggtctgaca tggtcaaagc ctcgctctgg tcgtgcgtca cgtagacgac ggtgaccgcg
    10621 agattctcgt gaatgtgctt gatctcgtac tgcatggtct cgcgcagctg cttgtcgagg
    10681 gcaccgagcg gctcgtccat gagcacgagc ctgggctcga acaccagggc gcgggccagg
    10741 gccacgcgct gctgctggcc gccagagagc tgggccgggc gccgcgagcc gaagccgccg
    10801 agctgcacca tgtccagcgc gcccttcacc cgcgcctcga tgtcggcctt ggccatccgt
    10861 cgcaccgaca ggggaaaggc gaggttctcg gccaccgtca tgtgcgggaa gagggcgtag
    10921 ttctggaaga ccatgccgat gtcgcgcttg tggggggcga cgttgttgat ctcgcggccg
    10981 tcgagataga tcccgccatt ggtggcggtc tcgaagccgg cgagcatcat caggcaggtg
    11041 gtcttgccgg agccggaggg cccgagcatg gtcaggaact cgccgcgggc tatgtcaagg
    11101 ttcaggtcct tcacgaccag cgtctcgccg tcgtagctct tctgcacctc ggcgaagcgg
    11161 acgaatgcct cttgctcgct ggtcatgggc actttcggct ctggtgtcga atcggcgcat
    11221 cccgtcgggt cggcgtgctc gcggcgcggt gtccctgtcg catcgcgccc ggatcgacgt
    11281 gttgacgacg atagagctag acaggatacc ggctgctgta aagccgacca ccaggcgggt
    11341 cgatcgaagt ggagatctcg cgatgaccat tccgctgttt cgcgacgacg cgtatttgcg
    11401 cgactgccgg gccgtcgtcg tggaagcgga tgacatgggt gtcgaattgg atcggtcggt
    11461 cttctacccg agcgctggcg gccagcccgg cgacaccggc tggctggtcg tcgggggcga
    11521 gcggctggcc gtcaccgaca cgcagaaggg ggcagggccg gataccatcc ggcatctgct
    11581 cgggcgaccg accgggctga ccgccggcat ggaggtgcat gcggtgctgg actgggagcg
    11641 gcgccatcgg ctgatgcgga tgcatacctg cctgcatctg ctgtgccggg ccgtcgacgg
    11701 cgttgtgacc ggcgggcaga tcggcgacgg caaggggcgg ctcgatttcg acctgccgga
    11761 ggcgcccgac aaggaggccc ttgccgaact gctcaatgcc tggatcgccg aggatcgccc
    11821 ggtcagcgag cgctggatcg atgcggacga gctcgacgcg cggcccgagc tggtgcgcac
    11881 gctctctgtc aagccgccgc gcggcgacgg ccgcgtccgg ctggtggaga tcgagggcgt
    11941 ggacctgcag gcctgcggcg gcacgcatgt gcggcgcacg ggcgagatcg ggccggtgcg
    12001 gatcggcaag atcgagaaga agggccggca gaaccgccgc gtgcaggtgg tgctcgggga
    12061 cgccggcgac gcaggtggtt gatccgggcc ctcccgaccg ccacatgccg cactgcacaa
    12121 tcgtacttgg ctaactcgag gagcgcttca tggccgaatc ctatcacacc gacctctaca
    12181 tcgacggggc ctggcacccg gccatggccg gcggccatat cgatatcctc gacccggcga
    12241 ccgaggaggt cgtggcgacc gcggcgcagg ccgagcgcgc cgatgtcgag gcggcgatcg
    12301 cctcggcgca ccaggccttc cagacctggc gcaaggtcct ggcctgggac cgctcgaagg
    12361 tgctgcgcaa ggcggcgatg ctgctcgagg agcgggccga ccggatcgcc cggctcctga
    12421 cgctcgaatg cggcaagccg ctcggccagg cgagggcgga ggtcatctcg gcggtcgagc
    12481 tgatcgactg gtacgccgac gaggcgcgcc gcatcttcgg ccagacgctg cccgggcgcg
    12541 gcgccgacaa ccgcttcatg acgatcttcg agccggtcgg cgtggtcggc gccttcaccg
    12601 cctggaactt cccggtcgtg ctgcaggccc gcaagatcgc cccggccctg gccacggggt
    12661 gcaccatcgt cacccggccg gccgaggagg gcacctcttc ggtggccgag ctggtcaagt
    12721 gcttcatcga cgccggggca cccaagggca cggtcaacct cgtcaccggc aagcccgagg
    12781 tgatctccga ggccatcatg gacagcgacc tcgtccgcaa ggtgaccttc acgggctcga
    12841 tcccggtcgg ccagcacctg atgcggcggg ccgccgacac ggtgaagcgg atgtcgatgg
    12901 agctcggcgg ccatgcgccg gtgatcatcc acgaggacgt cgacccggtg aaggcagcgc
    12961 tcgcctcggc cgccggcaag tcgcgcaaca acggccaggt ctgcgtctcg ccgacccgct
    13021 tctacgtcca caagaagcac gagcgggcct tcaccgacgc cttcgccggc gcgctggcgg
    13081 gcatgaagat cggccacggc ctcgagcccg gcaccgagat cggcccgctg gtcaacagga
    13141 agcgcctcgc cttcgtcgag gagatggtcg accagaccag ggcagagggt gcccgcgtgg
    13201 tgaccggcgg ccagcggccg gcggacatga accgcggctt cttcttcgag ccgaccgtgt
    13261 tcagcgacgt gcgcgacgac atgcgggtga tgaacgacga gcccttcggg ccgatcgccc
    13321 cggtcaccac cttcgagacc ttcgacgagg tgatcggccg cgccaacagc ctgagctacg
    13381 gcctcgccgg ctacgtcttc accggcaacc agaagctggc ccaggcgaca gcggaggcgc
    13441 tgcggaccgg catcgtcggc gtcaacacct tcgccgccgc gacggccgag atgccgttcg
    13501 gcggggtcaa ccactcgggc ttcggccgcg agaacggcag ccaggggctc tacgactacc
    13561 tggacgccaa gttcgtcaac atcgcctatg gctgaggtgt agccagcgat gacggagccg
    13621 cgcccgcggc tcaccgacct cgtccagggc ctgccggcga cgatcccgtt cgtcggcccg
    13681 gaggccctgg agcggcggcg cgggcgtctt ttcgagctgc gcctcggcgc caacgagagc
    13741 gccttcggcc tctcgcccct ggcggccgag gccatccggc gggagctcgc ccgggccgcc
    13801 tggtacggcg acccggaggc cttcgagctg cgcgagcggc tggcggggct gcatagggtc
    13861 ggcctcgatg agatcgtcgt cggcgccggc atcgacgagc tcctgggcgt gctggtgcgg
    13921 gcggtcacgg gcccgggcga tccggtcgtc acctcggccg gggcctatcc gaccttcaac
    13981 taccacgtcg ccggcttcgg cggcaggctg gtggccgtgc cctaccggga cgaccacgag
    14041 gacctggagg ggctcgccct cgcggtgcgg cgggaagggg cgaagctcgt ctatctcgcc
    14101 aatccggaca acccgatggg cacctggcac acgggttcgg ccgtccaggg ctttatcgcc
    14161 gggctgccgg ccgacgtgct ggtgctgctc gacgaggcct atgtcgagct cgccccggcg
    14221 gacaccgccc cgccgatcga cacggccgac ccgagggtgg tgcgcctgcg caccttctcc
    14281 aaggcgcacg gcatggccgg gctcaggatc ggctacgcca tcgcccatgc cgaggtgatc
    14341 caggccatgg gcaagatccg caaccatttc ggcgtcagcc gggtggccca ggcggcggcc
    14401 atggcctcgc tcgacgatgc ggccttcgtc gccggcgtgg tcgccgaggt ggcgcgcggc
    14461 cgggacgagt atgccgggct cgccgccggt ctgggcttca gggcgctgcc ctccgccacc
    14521 aatttcgtcg ccatcgacgt cgatcccgaa aagggtgggc cggagcgcgg ccgggcgctg
    14581 ctcgaagcgc tcgccgatgc cggggtcttc atccgccggc ccggcatcgc cccgctcgac
    14641 cgctgcatcc gcgtctcggt cggcaccgcc cccgagcgcg cgcgattcgc cgacatcttc
    14701 agccgcatcg tgcgcggttt ataggattta tagcctagtt cgggcatggt gtcaatcgcc
    14761 agccgggttc gctcggcaaa atcggctttt tgggggcgat gaaaccgggg cggcggcctg
    14821 atcgttgcga atcgccgggt tcgatcgtcg gattctttgg gttcgctcgt cgatttctcg
    14881 ggttcgctcg tcgattcggt gggttcgctc gtcgattccg cgggttcgtt cgtcgcccgc
    14941 tgtcgcggaa tccgcgacaa gatcgaatga atgtcgcgga atccgcgaca tcacgaatgc
    15001 ttatgtcgca gattctgcaa cggatcaggc ggccctgtcg cagaatccgc gacaacccga
    15061 gcgagaatgg cgcagaatct gcgacaagcg gtggcgccac ggcggaaaaa cagcacatga
    15121 cccgcgctcg accgcccgtt tcggcgaacc atcatcacga atgcttatga ccgcggatgg
    15181 tcgggcttgc cggcggcacg cgccatgcat ccctgaccgc gccctggcgc tgctcttcgg
    15241 cgtcgtgctc ggcgcgtcga tgggaaacgg cttgtcgatg ggctattgga cccgggtcca
    15301 gtcctggccc tcgcagatga agaggacgca gccctcgacg ttcagggtgt cgcccttcag
    15361 ggtcatgttg gacttgtagg tcttgtcgct ctcgggatcc cagatcgtgc cgtcggaata
    15421 gttgccgtcg ccttcggctt tcatgttctc gacgatcggc ttgccgagat tcgcgtaggt
    15481 cgggtcatcg ccgtccttgg tcaccgcctt gctgatcgtg ccgcaggtga gcgaggcatc
    15541 cgcggcgcag ggggccatgg tgatctcgat ataggcgccg tcttcgttcg cctcggactt
    15601 ccagacgccg tccacctcgg cggccagggc cgctgcgggc agcgccgcaa cagtcatcgc
    15661 cagcatcagc ttgcgcattc ttcggtttcc ttcccatggc agcgcgggct ccggccgttg
    15721 ccggccgggc accgggaggc cggatcgacc accgcgcaaa ggtgccgccg gtgccggcgg
    15781 cgatcaaccg aaacttcagg cttgcagtcg attccggagc ccttccggga gcgagggcct
    15841 agcgccggtc gagccgggcg agccaggggc cgaggcggtc gatggcctgc tcgatcgtgg
    15901 tgcgcgcacc ggcgaaggag aggcggacat aatggttgcc gcgggcgacg tcgaagtcgt
    15961 tgccgggggt gatggcgacg ccggtatcgg cgagcaggcg ggcgcagaaa tcgaccgaat
    16021 cgaggccgaa gcggccgatg tcggcataga ggtagaaggc gccgtcgggt gggaccaggc
    16081 ggtcgaggcc gatcctgggc aaggcatcga gcagcagcgc gcgattctcg ccgtagccgg
    16141 cgacccgggc atcgagctcg ggcttcgccc cgagggcgcc gatggcggcg tgctggccca
    16201 cggccgaggg cgagatgaag aggttctggg cgaggcgctc gaccgggtcg accagctcgt
    16261 ccggcagcac gagccagccg atccgccagc cggtcatcgc ccagtacttg gagaagctgt
    16321 tgacgacgat ggcgcgggtg gagctggcga gggcggtgct gcccggccgg ccgtaggtga
    16381 tgccgtgata gatctcgtcg gagatcaggc gcacgccacg ggcatcgcac cagcgggcga
    16441 tggcggcgag ctcggccggg tcgatcagcg agcccgtggg gttggcgggg ctcgcgacga
    16501 tgacgccgtc gagcggcccg ccgatcgcct cgaggtcggc cacggtcggc tggaagcccg
    16561 aggtttcgga ggtgaccagc ttcacgacct cgatgtcgag ggctttcagg atgttgcggt
    16621 aggcgggata gccgggctcg gccacggcga cgcgcgcccc ggcatcgaag gcggcgagga
    16681 aggccaggat gaagccgccc gacgccccgg cggtgatggc gatgcgctcg atgggcacgg
    16741 cccgggcgta ggtggcctcg atgtggcggg cgatggcggt gcgcagcggc ctgagcccga
    16801 aggcctcgga gtagcccagc gcgctgccgt cgagggcggc gcgggcggcg tcgagggcgg
    16861 cccggggagc accgcccgag ggctcgccca cctcgaggtg cagcacgtcg ccgccctcgg
    16921 ccgcgcgctt gttggcggcg gccagcacct gcatcaccag gaaaggctcg atggcgctgc
    16981 gcccggagac acccgagcgc tccggctcct tcggtagtcg catgcttcac ctcgctcggc
    17041 gccggccgga agccggcatg ggggctcggc gccggccgga agccggcatg ccgtgtcctt
    17101 agcagagaat ggtcaaggcg gggcgaaggg cgagggcgga tcgagcgttc ttgatggagg
    17161 gtgaggtcga cgggagcggg gccgcagccg gaacggtggc gccgacgtcg ccgatccgat
    17221 acggatcggt ccatggctga cgagcaaacc aggcggcggc tggccgcgat cctcgccgtc
    17281 gacgtggtcg gctattcccg gctgatggcg accgacgagg cgggcacgct ggccgcgctg
    17341 aaggcgcatc gcagggccgt gctggagccg gcgatcgccc tgcggggtgg gcgcatcgtc
    17401 aagctgatgg gcgacggcgt gctggtcgag ttttcgagcg ttgtcgacgc cgtcgcctgc
    17461 gcgctcgcga tccagaccgc cgatcagggc ggggagtcgg ccggcgccgg cgcgctgacg
    17521 ctgcgggtcg gcatcaatct gggtgacgtg atcatcgacg gcgacgacat ctacggcgac
    17581 ggggtcaatg tcgcctcccg gctcgaggcc ctggccgagc cgggcggcat ctgcatctcg
    17641 tcgatcgtcc acgagagcct cggcgggcgg gtcgaggccg gcttcgtcga tggcggcaag
    17701 gtccaggtca agaacatcga ccggccgatc ggtgtctggc ggtggcaggc cgggtcggcc
    17761 ggcgcggcgc gggcggcgcg cgtggtgccg ccggcggcgg caacaaaggc atccgtcgcc
    17821 gtcctgccgt tcgacaacat gtccggcgac ccggagcagg agtatttctc cgacggcatc
    17881 agcgaggaca tcatcaccga tctctcgaag atcggcgggc tcgtggtgat cgcccggaac
    17941 tccagcttcg cctacaaggg gcggcgcgtg gacatccgcg aggtggcgag ggacctcggc
    18001 gtcggcacgg tgctcgaggg ctcgatccgg cgggctggca accgggtgcg catcaacgcg
    18061 cagctcatcg aggcggccac aggtgcccat ctctgggcgg agcgctacga ccgcgagctc
    18121 accgacatct tcgccgtgca ggacgacgtc acccggcaca tcgtcgacgc gctgaaggtc
    18181 gagctgaccc ggggcgagga ggcgcggatc gccgccgttc ccaccatgag ccacgaagcg
    18241 cacgacctct tcctgcgcgg ccgcgagcac atccgtcgcg gggtgtggac ccgggcgatc
    18301 ctcgaagagg cggtccggac cttcgggcag gcgatcgcgc tcgatgcgga ctacgcggac
    18361 gcccatgccg gtctcgcctt gtgccacagc atcgatttcc agaaccggct gaccggcgac
    18421 ccgacggccc tcgcgacggc ggggcggatc gtcgaggtgg ccatcgcgaa ggggccggac
    18481 ctgccctttc cgcactgggc cgccgcggcc atcgtcatct ggcagaagga cctggcgcgg
    18541 gccaggcgcg agtgcgaggc ggcgctggcg ctgagcccga actacgcgct cgcccacgcg
    18601 acgcgcggcc atgccgagat ctatgccggg acgccgctga aggcgatccc gtatctcgag
    18661 cgggcgatcc ggctcgaccc cgccttcacc cagcaatacc tgcatttcat cggctcggcc
    18721 tatctggcgg cggggcgcta cgaggtggcg gccgcgacct tccgcgagcg catccggctg
    18781 gcgccggaga cggacatctc gcggggcctc ctgatcgcgg cgctcggcca tctgggcctg
    18841 gtcgaggagg cgccggaggt ctgggacgag ctgaagcggg tcaatccggg ctattcgttc
    18901 gaggcgcatc tggcgcggct gccgttcagc gacccggccg acgcggcgcg catcgcggcg
    18961 ggcttcgcgc gggccggcgt ggccggctga tcgcggcgct caggccttgc agggcttcga
    19021 gttaaaatcg gttcgtgtcc cgactttatt tcggttcgtg tcccgacttt atttcgaaaa
    19081 tcgggacacg aaccgacttt aacgatcgga ctcacccgtt tgcggggctc tgtcggccgg
    19141 gctctcattc gtgcccggcg ggcggcccag cctggcgtgg cgcagcttgc ggccgagggc
    19201 cgcttcgagg cgttcgagaa aggccgtcga gccgaggggc cggccacttc gggaatggcg
    19261 gcgcagctcg tcgagctgcc agctcggcgg cgtgtcgtcg agaaaggccc gccagtcggg
    19321 cacgagatgg tcgaggccgt gcggctcgac gaggccgtcc gcgccgaggc cgagatgggc
    19381 tcgagcactc gaccagggcc aggccgcggc gtcggcgacg aggccggcgc gctccgggtt
    19441 gcgctcgaca tagcggatcg cggcgaggca gtgcggctcg tccatggcgc acgagccgaa
    19501 gcgtccctgc cagagatagc cggtccagcc gtgccggcga ttgaccgccc gggtgtagtc
    19561 gcggtgggtg tcgccgatgg ccctggccaa gctgtccggc gcatcgggga cgacgaccag
    19621 atgcacgtgg ttgggcatca ggcaccaggc ccagacggcg acccgggccg ctcggcaatg
    19681 ggtccgcagc aggtcgcggt agaggcgata gtcggcatcg gcgaagaaca cggtctgacg
    19741 ccgattgccg cgttgggtga cgtggtgcgg cacatgcgga gccaccgctc gggcgatcct
    19801 tggcatgcgg caggcttagc agggtgaggg cggcgttaaa agcggttcgt gtcccgactt
    19861 ccgggggtta aaagcggttc gtgtcccgac ttcgagcgcc gacttcgagc gaccgacgtt
    19921 cacgccttgc agagcttcca ggggccgatg tcgacgtgga tgtggtcgcg gtgcgcccgg
    19981 tcgtggttgg gggtgagcac gacgttgaag tggcggcagg cggcccgggc gaggtcgtgg
    20041 aggaagaggc tggcgccgcc gctgcggcgt ggccagtcgt cggccacgct gatctgccgg
    20101 ccgtcggcgg tggtgaagcc ggcgatgtcg agcgcctcgc cggtgctgtg caggctcatc
    20161 cgccgctggt tgccggtcat cttgcggcag ctgtaggagc cgtagtggcg gatctcggtg
    20221 atgcggctgc cgaggcgccg atcggcgagg cgctgcatct ccggctcgaa gcgcgcccag
    20281 gccagcagca tcgggcagct cgtccggagc gtcggcgcca ttgcggcgct ggtccaggtg
    20341 accacgaccg gtgccggcac ggcgcaggcg ccgtcggccg gagcaccggc ccagggggtg
    20401 aagcgcacgc cctcggtggc gagcagggcc cggcaggcgg ccgggtcgct cggccagcgc
    20461 gtggcggcct ggcgggatgg cggcgggccg gcgcaggcgc cgagcaggag gccggccagg
    20521 gcgacgagag cgacgttcct cgtcagccaa ccggctcccg cgcggtctcg gcgatcggca
    20581 ggtccggctc cttgatcgcc atcttgcgct gccagaggaa gacggcgccg ccgaggccga
    20641 tgccgacgag gtcggtgacc agcccgccct cgatcatcaa aagcgccgcg gcgccgatca
    20701 cgaggcggag caggatgctg gtgcggccga agaaccagcc ctggacggcg gccgagagca
    20761 tgaagacccc gacgcaggcg gtggcgaggc tctggatgat ggccggccag gtgtccatca
    20821 tcagcaaggc cgggctgtag aagaacatga agggcacgat gaaggcggcg aggccgagct
    20881 tgaaggcggt gagcgaggtc tcgagcgccc tggagccggc gatggcggcc ccggcatagg
    20941 cggcgagcgc cacgggcggg gtgatcgccg agatgacggc gaagtagaag acgaagaagt
    21001 gcgcaatcag gggctcgatg ccgaggcgga tgaggccggg ggcgacgacc gaggcggcga
    21061 cggcgtaggc ggccgtcgtc ggcatgccca tgccgagcat gagcgagatg cccatggcga
    21121 agagcaaggc caggaactgg ctggtgtcgg cgacggtgag cagcatggcg gagaagcgca
    21181 ggcccacgcc ggtcaggccg atgacgccga cgatgatgcc ggcgcaggcg cagacggcga
    21241 tgagctggac cgccatggtg gcggtcaggt tgagcgcctt gacgatgccg acggggccga
    21301 gccggtgcgg cgtgagccag ctcaccacga ccgcggcgac gagcgagagc gtgccggcgc
    21361 ggatcaccga gtagcccatg aagagggcgg cgatgaggat gacgatcggc acgaagagat
    21421 aggcctgctt cgccaggacg tcgaagcgcg ggagctggtc ccggggcagg cccttcatgc
    21481 cggtgcgctg ggcctcgagg tcgaccatga agtagatcga gatgaagtag agggtcgccg
    21541 ggatcagggc cgcgacggcg atctcggtgt atctgatgcc ggtgacctcc gccatgatga
    21601 aggcgccggc gcccatgatg ggcggcatga tctggccgcc ggtggaggcc gcggcctcga
    21661 tgccggcagc gctgcgcgcc cggtagccga cgcgcttcat gagcgggatg gtgagcgagc
    21721 ccgtggcgac gacgttgccg gccgaggtgc cgttgatcat gcccatgagg cccgacgcga
    21781 agaccgcgac cttggccggg ccgccgcgcg catggccggc ggcggcgaag gcgaagttga
    21841 cgaagtagtc gccgaccttc gacgcctgga ggaaggcggc gaagcagatg aagaggatga
    21901 tgtaggtcga ggagacggcg gtggtcggtc ccaggatgcc gttgtcggtg tagagatagg
    21961 tgaagaagcg atccgcgctg tagccgctgt gggcgaggaa gcccggcagc caggggccga
    22021 cgaaggcgta gaggatgaag gccatcgaga tgacgacgag cgcgaggccg gcgaggcgcc
    22081 gcgtgcattc gaggatcagc gccacgccgg ccagcgtcac ccagaagtcg gggcgggtcg
    22141 gcatcacgcc ggcgcggaac tgcaggccct ggaggttgaa gaggaggtag agcgcgaccg
    22201 ccacggcggc ggcggccatg gtccagtcgg ccgggtcgat gcggctggtg tcggccgggg
    22261 tcagccaggc gaggagcatg gcgaggccgg tgccggcgag cagcgccggg gcgccgatgg
    22321 cgtagaccca ggggggcggg ttcggcaggc cggcgagctc gcgcaagccg aagggtgcca
    22381 gcatggcgag gagggcgacg gcgaccagca cggcggcgac gaggcggagc gggcgcagca
    22441 gctgctccgg caggccgccg cccgggcgct cgagccgggg caccgcgtgg gtggcgacca
    22501 gaaagccgat gccgaggccg ccggcgacgt gcagggttcg aaaggcccag gtctcgagcg
    22561 ggaagacgtt gagcaccagg aggtgccagg aggtgtaggc gacgcagtac cagaagacga
    22621 agtggtggct gaagccggtg aggctgcgct ggttgccgga cgccggctcg tcgtcgacgt
    22681 cgcgggcgat gatgagctcg ccgtccgtgc cgttcgccat gggccgcctc cccgactggc
    22741 cttctacagc agaagggcgg ggctggcgcc ccgccctcga tcgctcagcc ctcgtactcg
    22801 gccgggatca ggttctccgg gatgtcgaag cccttctcct tgtagtactg cacggcaccc
    22861 gggtggaacg gcaggaactt gttgtgcacc cagttctcgg gcagcgtctc ggcggcggcc
    22921 tggtggatct gcatcatccg gtcgttgtta tcgagcacga ccttcatgac ctcgtagacg
    22981 aagctctgcg gcaggtcctt gtgggcgatg ccgaagttcc acatggcgac cgagtgctgc
    23041 ggctcgtgct ggctggtgta ggtgtcggcc gggatctcga aggcgctgac cgggaactgc
    23101 tcgacgaggg tcgcctgctc ctccggcgtg aaggcgaaga tgttgaccgg gttctgggcc
    23161 tcgagctcgc tgaaggcggc gatcggcagg ccggcgcaga aggcgaaggc gtcgatcagc
    23221 ccgtccttga cctggccggc gaggtcggag gcgccgccga actggtcgtt ggtctcgacc
    23281 ccgagcgcgt cgaaatattg cgggaagtag gtgccgcagg tgccgccccg ggggccgttg
    23341 ccgacggtct tgccgtcgag atcggcgacc gactggatgc cgctggattc gagcgcgatg
    23401 atctggaagg gcgtctggta catcgggaag gtggcgcgca cgtcgcgcat ctggacgccc
    23461 ggggcgatcg ggctcaggcc gtcccaggcc tcgcgtgccg ggcccatggt ggtcatcgcc
    23521 atctggacgt cgttggtctg ggtcagcgcg aagttctgca ccggaccgcc ggtcacctcg
    23581 gagctcgccg gcacacccag catctcctgg atcagcccgg cccagccggc gccgtagatg
    23641 aagtaggtgc cgccctgcga tgccgtgccg accttgatcg acgacggcca gccggtcttg
    23701 tcctgggctg tgccggtggc cggtgccagc gccaggacgc ctgcgagggc cgtcatgccg
    23761 aggccggtga ggcaggttct catcatgtga tttctccctg aacgagtgtt gcgccgcagg
    23821 tggtcgcggc tgggtgcgtc aaagactagc ggaccgcgag cgtccggcaa gccggcaaca
    23881 tgtcgccggc cagaggcgcc cggctcaggg catattcagg agcagcccat cgaggaaggc
    23941 cacgatctcg ggggcagccc agtcggcata gccggcgtgg cggaagatct cgttgccctg
    24001 gccgtcgatg acgagggtca ccggcaggcc cggaatgccg aggggatggc tcgttgccat
    24061 ctgcggatcg cgccagatgg tgaggtcgtc gaccccgata tcggtcatga aggcctggag
    24121 cgcctcgggg ccggcgcggt ccatggccag ggtgacgacc ctgagcggcc ggccctcgaa
    24181 cgccgcctgc agggcggcga gggtgggcat ttcacgcttg cagggcgcgc accaggtggc
    24241 ccagaagttc agcacgacga cctcgccggt ggcgaccgcg gcagcgagat cgaagctctg
    24301 gccggccatg tcccggatct cgaccggggg caccggcacg ggctcgccgg ccacgggctc
    24361 gagggccgct gctggtgccg cgaggccgag gagcaaagcg agagcggcgg ggaatcgggg
    24421 catgggcggc ggcctcacca tggcaattga gcggcaacgg gatccggacg ggcgatgacc
    24481 agagacacga aagcggcgcg cgacctctgg ggcgggcatt tcgcgtccgg cccggcgccg
    24541 ctgatggaga agattaacgc gtcgatcggc ttcgacaagc ggctctggcc gcaggacgtg
    24601 cggggctcga tggcgcatgc gcggatgctg gcggagcgcg ggatcatcgc gccggccgat
    24661 cgcgacgcca tcatcgaggg gctagaggcg ctcgaggcgg cgctcgaaga aggcagcttc
    24721 gtcttcgatg ccaagctcga ggacatccac ctcaacatcg aggcgcggct gaccgagcgg
    24781 atcggcgagc ccgggcgccg gctgcacaca ggtcgctcgc gcaacgacca ggtggcgacg
    24841 gacttcaagc tcttcgtgcg cgacgccatg gaccgtgccg tggcgctgct gaagggcctg
    24901 gtgggcgtgc tggccgaccg ggccgaggcg gaggcggcga cggtgatgcc gggcttcacc
    24961 catctgcagg cggcgcagcc ggtgaccttc ggccatcacc tcctggctta cggcgagatg
    25021 ttcatgcgcg acgtctcgcg cttctcggat gcgaggcggc ggctgaacga gtgcccgctg
    25081 ggtgcggccg ccctcgccgg cacgagcttt ccgatcgacc gggcccagac ggcggcagcg
    25141 ctcggcttcg ttcggcccgc ggcgaactcc atcgacgcgg tctcggatcg cgacttcgcc
    25201 ctggattacc tcgcctcggc cgcgatcgcc gccatccacc tctcgcgcct ggccgaggag
    25261 ctggtgctct ggtcgacgcc gcagttcggc ttcgtcaaga tggccgaggc cttcacctcc
    25321 ggcagctcga tcatgccgca gaagcgcaac cccgatgccg ccgagctggt gcgcggcaag
    25381 agcgggcggg tcatcggcca cctgcaggcg ctgctggtga ccttgaaggg gctgccgctc
    25441 gcctactcga aggacatgca ggaggacaag gagggggtgt tcgacgcggt cgacacgctc
    25501 gagctctgcc tcgaggccac ggccgccatg ctcgcctcgc tcacggtcga cccggcgcgg
    25561 atgcgggccg cggccggggt cggctacacc acggcgaccg acctcgccga ctggctggtc
    25621 cgggtcaagg gggccgcctt tcgcgacgcg catggcagcg ccgcccgggc ggtgaaggcg
    25681 gccgaggagc ggggctgcgg gctgcacgag ctcgatgccg acacgctgcg cgccatcgac
    25741 ccgcggctcg agccggcggc ccttctcgac gtcgacgcct cggtggcgag ccggacgagc
    25801 ttcggcggca cggcgcccga gcgggtgcgc gaggcggtca ccgcactgcg cgggcggctg
    25861 gcggagctgg cgcgatgagg cggcgggcgc tgctcgggct cgtggcggtc ggcgtgctcg
    25921 ccgcctgcgg gcggcgcggc ccgctccgcc tgccggacgc cccgccgctg gagcagccgc
    25981 cggtggacaa ctcgatccgg gacatccccg agccggaacc gacggacgac gggacggccg
    26041 ggtgagggcc ttcacctatc gcgatggcgg gctggcctgc gagggcgtgc cgctcgaggc
    26101 gatcgcccgg gcggtcggca cgccggccta tgtctactcg gccggcgcca tgcgtgcccg
    26161 tctccgggag ttccaggacg ccttcgccgc ccggccggcg ctcttctgct atgcggtcaa
    26221 ggccaaccac aacctcgccg tgatccgcac gctggccaat gccggggccg gcgccgacac
    26281 agtctccggc ggcgagatcg agcgggcgct ggcggcgggc gtgcggccgg agcgcatcgt
    26341 cttcgccggg gtcgccaaga ccgatgacga gatccgcttc gcgctgcgaa cgggcgtgct
    26401 ccagctcaac gtcgaatccg tgcccgagct cctgcgcatc gatgccgtgg cgcaggggct
    26461 cgggctgcag gcgccggtgg cgttccgcat caacccggac gtcgatgccg gcacgctgag
    26521 caagatctcg accggccgca agggcgacaa gttcggcgtg ccgttcgacg aggcggccga
    26581 cctctacgcg ctggccgcct cgctgccggg catccgcccg gaggggctgc acctgcatat
    26641 cggctcgcag atcacccggc tcgacccctt cgccgccgcc taccggcgcg gcatcgagct
    26701 cttccggacg atccgcgccg ctggcatacc gctccgtcgg ctcgatctcg gcggcggctt
    26761 cggcgtgcgc taccgcaacg aggagccggt gccggcctcc gccttcgcgg ccctggtcca
    26821 gggcctcacc gccgggctcg acgtcgagct ggtgttcgag ccgggccggg cgctggtcgc
    26881 cgaggccggc gccctggtgg cgtcggtgat ctatcgcaag gagagcggcg ggcggcgctt
    26941 cctgatcctc gatgcgggca tgaacgtgct gatccggccg gccctctacg acgcctggca
    27001 cgacgtcctg ccgctggcag agccgccgcc taaggcgcat ctcgagccca tggacgtggt
    27061 cggcccgatc tgcgagagca gcgacatctt cggccgcgac cgctacctgc cgctgctcga
    27121 tgccggcgac cgggtcgcct ttctttcggc cggcgcctat ggttcggtca tgtcttcgga
    27181 ctataattcg cgggtgagcg ccgccgaggt gctggtggac ggcgaccggt gggccgtggt
    27241 caagccgggc cgcgagccca gtcggcagtt cgccgacgag acgattccgg actggctggc
    27301 ggccgggtcg gagggacgat gaggtcggcc cggggagacg ggccgcaggc gaggggtgag
    27361 cgcatggccg agggcgccct gccgatgtcg agccgcagct tcaggcggaa gctcggcctc
    27421 gcccgcctcg cggtcagctt cgagcggctc tatcctgccc tcttcccggc gctcctggtg
    27481 gtcgccggct ttctcgccct cggcctcttc gatttctggc gcatggtgcc ggtctggctg
    27541 cacgtggccg ggctcctggt cttcgccctg gcgctgggct ggagcatcta ccgggtgcgg
    27601 gaccggttcc gcctcgcctc ccgcgatgcc ggcctcgccc ggctggagcg cgacagcgcg
    27661 gcccgccacc agccgcttcg ggcgctcgag gaccggctcg ccgacgactg gcaggatccg
    27721 gcgggccagg cgctctggcg gcggcatcgc gagcgcgtgc tggcggcgct cgggcgggtt
    27781 cgcctcgccc cgccgcgctc cgccctgccg gcccgcgatc cctgggcggt ccgggcgctg
    27841 gtcctgctgc tgctggtcgt ggccgtggtc gatgcccgga ccgatgccgg ccggcggctg
    27901 gtgcgcgcct tcgtgccggg cacggtgggt gccgcggccc tgccgatcga ggtgagcctc
    27961 tgggtgaccc cgccgcatta caccggccgg ccgcccctga cgatcgagca gacgcgccgc
    28021 ctcgagcgcc tcgacgtgcc gatcggcagc gaggcggtgc tgcagctcca caacctgccc
    28081 gcgggcgatg gcgggcggcc cgaggtcctg ttcaacggcg aggcgctggc ccaggaggcg
    28141 ctcggcgagc ggagcggcga ggtccgcctc gacctcgtcg aggccggcca gctcgaggtg
    28201 ctcaaggccg atggcgagcc gctcgccggc tggcaggtgg cggtgctggt cgaccacccg
    28261 ccggtgatcg ccttcgccga gccgccccgg gcgacgcatc gcagcgtcct cgagctcgcc
    28321 tacgcggcca gcgacgatta tggcatcggc ctggtggcgc tggagctcgc gctggtggag
    28381 cggcccgagg agcgggaccg tgcggtgctg gcggagccgg cgcgcgcccc caccgaggtc
    28441 gcgagcacga cctatctgga cctgaccgcc catccgctcg ccgggctcga ggtggcgttg
    28501 aatctcgtcg ccgaggacgg gctcggccag gtggggacga gcgagacggt ggtgatggtg
    28561 ctgcccgagc gggtgttcga gcaccccctg gcgcgggcgg tgatcgccga gcggcggcgg
    28621 ctggtgcgcg agccgggcgc gcggcgggcg gtgaccgcgg ccctgggcga gctggccgag
    28681 agtcggctcg ccgagcaggc cggcacggcg gtccagctcg ggctggcggt ggcgcaggcg
    28741 cggctgcgcg gtgcccgcga cgcggcggag cggcgctcgg tcgtcgatct cctctgggag
    28801 ctggcgctga tgatcgagga tggcggcctc tcgatggccg agcggtcctt gcgcgagatc
    28861 caggaggccc tgcagcgggc gctggaggaa ggggccagcg aggccgagct cgaggcgctg
    28921 atggacgagc tccgcgaggc gatggaccgc tatctcgagg agctgacccg ccaggccctc
    28981 gagcagatgc ggaacatgcc gcccgacatg cggatgcagc ccatcgaccc gtcgcagatg
    29041 gtcgaccgcc agtcgctgca ggagatgatc gaccgggccg aggagatgat gcagagcggg
    29101 gcgcgcgagc aggcccaggc gatgctcgag cagctgcagc agatgctgga gaacctgcag
    29161 gccggggtgc cgcagatgca gccgcatccg ggcgagcagg cgctctccga cctgcagcgg
    29221 atgatcgagc tgcagcagaa cctcctggat cgcagctacc agatgcagca gggccagccg
    29281 gggcagcaga gcgagcccgg ccagcagggg cagcaggggc aggaaggcca ggaggggcag
    29341 cagggcagcg ggcaggcggc ggccgagcag gacgccttgc gccgggcgct gggcgagctg
    29401 atgcgccggc tcggcgaggc cgggatggac atcccgaggg cgctcggtca ggccgagctg
    29461 cagatgcgtg gcgcgcgcga tgcgctgcag gagggccagc ccggccgggc ggtcggcccg
    29521 cagggcgacg cggtcgacct catgcagcag ggcgggcagg cgatgctcga cgaattgcgc
    29581 gagcagatgg cgaaccagcc gggcggcggc cagggcatgg ccccggggca gatgggccgg
    29641 cagcgcgagg gccgcgaccc gctcggccgg gcgagccgca acgagggcgg ctgggacagc
    29701 gagggcacgg agatcccggc cgagaacgat ctcggccgcg cgcgcgacgt gctggaggag
    29761 ctctaccgcc gctcgcgcga ccagggccgg ccgcagcagg agctggatta cttcgaccgg
    29821 ctcttggagc gcttctgacg caagcaccgc gcccgctgcc gtcgcccaac cgggacgccc
    29881 ggccggcggg tgccggcgtg gcgctgctcg tcctgcacta tacgggcatg cggtcggcgg
    29941 cggcggccat cgcccggctc accgatcccg cggccaaggt cagcgcgcac tacgtgatcg
    30001 acaccgatgg caccgtcctg gagctcgtgc ccgaggcctg ccgggcctgg catgccgggg
    30061 tggcgggctg gggggcgatc cgcgacgtca acgggcagtc gatcggcatc gagctggtca
    30121 atcccgggca cgactggggc taccggccct ttccggcggc gcagatcgcg gccctcgtcg
    30181 agctcggcca ggcgatccga gcccggcacg ggttgccgcc caaggcggtg gtcgggcaca
    30241 gcgacgtcgc cccggcgcgc aagatcgatc cgggcgagct cttcccctgg gccgacctcg
    30301 cccggcacgg gctcggcatc tggccggcgg catgggagaa cgaggcgccg gacacgggcg
    30361 cggcgctcga agcgctccgg gcgatcggct accggccgga cctgccggac accacgcctg
    30421 ccagggtgat cgccgccttt cagcggcact ggcggccgga ccggatcgac ggccggctgg
    30481 acgaggcgac gatgggcctg atcgcggccg tgcatcgctt gtccaaggcg gcgccggacg
    30541 ctacatgaag tgccgggtgc gtggcggacg ggtggccgct cgcgaccgga cggggcgacc
    30601 cggccggcag cgggaggaaa gtccgggctc cacggaaaca cggtgccggg taacgcccgg
    30661 cggggg
//
LOCUS       DAOTFI010000017        30069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1723_length_30069_cov_6.854168, whole genome shotgun sequence.
ACCESSION   DAOTFI010000017 DAOTFI010000000
VERSION     DAOTFI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 30069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30069
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1723_length_30069_cov_6.854168"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="P5558_02805"
     CDS             complement(<1..1461)
                     /locus_tag="P5558_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY23272.1"
                     /translation="MLVRVGEKRVMLCDCEATMPLDAEALARALGTETPAIATSLCRR
                     QQARLAGEAPGIVACAQERAVFEEALTEAAGHAAAESLVTVDIRARAAWSKEAAEATP
                     KIAALLAEAALPVPETHVVTLESGGVTLVYGRDEVAIEAAAQLGDRLDVTVLLTRPGD
                     VTPPARTTFPVLQGTIRTARGHLGAFELVVDDYALPAPSSRRRLRFGPARNEAVSNCD
                     IILDLTGGTPLFPSPRTRPGYLHADPADPVAVQKALLAATDLVGTFDKPRHVAYRPEL
                     CAHSRNRTVGCSRCLEVCPTGAITPNGDHVAIDAAICAGCGGCHAVCPTGAASYAYPP
                     AEHLLERVRTLLTTYHAAGGTDAVLLLHDAEHGAALIDLLARVGDGLPARVIPVAVNE
                     ITQLGIELLAGAMAMGAADIRILATGRRRPDLTPLARVMGLAEASLAGLGYGTGRVQL
                     IEADDPDSLGEALWQLERRPGTATPASFLPMGDKLGL"
     gene            1494..2123
                     /locus_tag="P5558_02810"
     CDS             1494..2123
                     /locus_tag="P5558_02810"
                     /inference="COORDINATES: protein motif:HMM:NF028227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HRY23273.1"
                     /translation="MNAMRMADNPRTLPDLPPPFRVRWVAGDVMAAARQAAERGEEPG
                     LLLIGEDEATCDLALALAPDRPEAACRVVLHLAMLAVADALAAVGPPLKEVVFASPDG
                     LRLDGAKVGRVRLAMAEVAAPDEVPEWCVVGPEVRMMLPPAAGEPGAMPEVTALQEEG
                     FGGVGVVELAQSFARHFLYWMHRWTEEGSGPVLAHWRARLDPLTIEPVL"
     gene            2120..2614
                     /locus_tag="P5558_02815"
     CDS             2120..2614
                     /locus_tag="P5558_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HRY23274.1"
                     /translation="MTLKLPRTIRLDASDEAVFERAAEPGEWAVAGGFAFLDTDPTAL
                     AGKRRQAFVSGFLGTESRGWSTFVVVATADAAAEAEMVERLARLFVAAHGAPDLDAAR
                     PVARQEVELMAGLCRDHAPGTLFAITRSVDAEGGIRESFRAVDRKAGVHGRVWDVVPD
                     EGQG"
     gene            2611..3633
                     /locus_tag="P5558_02820"
     CDS             2611..3633
                     /locus_tag="P5558_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HRY23275.1"
                     /translation="MSGAIWASAGRELLDPAAGGGLAVTASFLKAYLARPELAPVAES
                     CTAERALHARLLADPFAAVEDAALADLADADAAQNWRVWLGFRDFLARRPSLEAAYLA
                     LVKGEGPAVPALFVDQLVHVILRHLLDGEADAYRWRAAECLFRPQKVSITEGGILLAD
                     EETVEQAAATGGFGGLGQLLRQAGTVPRSVELDVLGEGNAASYAGRSERFDMVLDVAF
                     TRPGSDALARVLERWVAHFLSLAIRLQPVQTIRDESWAWHVGLDVEATGILNAMWEGV
                     EVPQADLARILGLFRLEFLDPSAMLERVAGRPVYLAMAMDCEKRLRLKPQNLLLGLPL
                     RQDRAS"
     gene            3640..4143
                     /locus_tag="P5558_02825"
     CDS             3640..4143
                     /locus_tag="P5558_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HRY23276.1"
                     /translation="MATDRMSVAVIVERRPAGSRWASHVWRAVEVLPGAPATPAWTVL
                     AEGGGATRFFAGVAELVVHSGETEPLAHNLGAAVPSVWVVLRRAATPPGWRLLLATVD
                     AGEAHAHADTGDDLVEMVAMPDAVRAWLEPFVARHHVERQRHKRRRDRADPEALAVGR
                     RGGGEDG"
     gene            4136..4693
                     /locus_tag="P5558_02830"
     CDS             4136..4693
                     /locus_tag="P5558_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HRY23277.1"
                     /translation="MAEEERPFLARWSERKRQAQAREAPSEAVPLPVESEAAEPEPDF
                     DPASLPPVESLGPGSDIAAFLRKGVPAALRRAALRRIWTVDPKIKGFREVADYDWDFN
                     APGYGAISPTDDARQAAERLLARLRRPAAEPASAEDPEAAPTEHVVAEEGERPAALPV
                     EPEASGPEWEPEPVARRRHGGATPR"
     gene            4807..5412
                     /locus_tag="P5558_02835"
     CDS             4807..5412
                     /locus_tag="P5558_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HRY23278.1"
                     /translation="MSEPGNMANGAAGDVELARARLWQLLGLCLRAAPDAATLRLIAG
                     LQGDPSPLGSTLGELAGLARSADPVLLAREHHDLFIGLARGELVPYASYYLTGFLHER
                     PLARLRGDMAGLGIARTPGLAEPEDHIASLAESMAGLIAGDFAADAAAQQRFFERHIA
                     PWAGRFFADLEVAQAARFYRPVGALGRHLIEIETTAYALAA"
     gene            5445..5660
                     /locus_tag="P5558_02840"
     CDS             5445..5660
                     /locus_tag="P5558_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23279.1"
                     /translation="MAKSPANTEAPASPARRGFFKAAAGGAAGAALVVPAVLGGGARA
                     AESGEEQRKTRYQETDHVKTYYQTNRY"
     gene            5678..8599
                     /locus_tag="P5558_02845"
     CDS             5678..8599
                     /locus_tag="P5558_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRY23280.1"
                     /translation="MLQKRRAGVSSKTGLAGVLAAAGSATVDRRAFLRKSGVAAGGLA
                     ALGAVPLATVRKAEAGPMQPGVPIEIKKNICTHCSVGCTVTAEVQNGVWVGQEPAWAS
                     PINRGTHCAKGASVREIAHGDRRLKYPMKLSGGEWQRLSWEQAIDEIGAKLEELREES
                     GPDSVFWLGSAKFSNEGSYLFRKLAAMWGTNSVDHQARICHSTTVAGVANTWGYGAMT
                     NSYNDIANSKAIMIIGGNPAEAHPVSLQHVLRGKELNQAPMIVCDPRFTRTAAHATHY
                     VRLRPGTDIPLVWGMLWHIFENGLEDKEYIAQRVHGMDQIRAEVAKWHPAEVERVTGV
                     PGAQVAEIARIMATQKPSTLIWCMGVTQHTVGTANVRALSILQLALGNIGIEGGGANI
                     FRGHCNVQGATDLGLDVTSLPAYYGLSERAWRHWSRVWDLDYEWVRDRFGKAGMTVED
                     KQKLMEAKGIPTTRWFDAVLADPADVDQPDRLRGMVVFGHGGNTVPRMPEMRAGLEAL
                     DLLVVADPHPTTFAAVSERKDGTYLLPICTQFECNGSRTASNRSLQWGNQIVEPLFES
                     KNDYEAMYLLSKRLGIEEQMFKHIQVDGTAPLAEDILREINRGTWSIGYTGQSPERLK
                     QHMEHQGDFDMVSLRGKPGTPVEGEVYGLPWPCFGTPEIKHPGTHILYNTHEHVMNGG
                     GTFRARFGVERDGETLLAEGSWSKGSEIEDGYPEFTYGILKTLGWDGDLTAEELATIQ
                     KIGGDDPDSVSWAIDLSGGIQRVSMAHGCVPYGNAKARAVAWNLPDPVPVHREPIYSP
                     RPELVAEYQTYEDKRDFRVMNIGHTIQAQAVKEGTAGSYPIILTTGRLVEYEGGGEET
                     RSNKWLAELQQSMFVEVNRADAARLGVKDGGMVWVYGPAEGKVRVKALVTNRVGEGVA
                     FMPFHFSGYWEGESQRAKYPEGADPIVLGDSANACTTYGYDPVTAMHEGKATLCRIEA
                     A"
     gene            8626..9225
                     /gene="fdh3B"
                     /locus_tag="P5558_02850"
     CDS             8626..9225
                     /gene="fdh3B"
                     /locus_tag="P5558_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HRY23281.1"
                     /translation="MARMKFLCDAERCIECNACVTACKNENEVPWGINRRRVVTLNDG
                     EMGKERSISMACMHCTDAPCAAVCPVDCFYTTADAVVLHSKELCIGCGYCFYACPFGA
                     PQYPQLGNFGGRGKMDKCTFCAGGPEADNTPAEYARYGANRLAEGKLPLCAEMCSTKA
                     LLAGDGEIIAAIYKERVVTRGYGSGAWGWSTAYPDSQGV"
     gene            9248..10249
                     /locus_tag="P5558_02855"
     CDS             9248..10249
                     /locus_tag="P5558_02855"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HRY23282.1"
                     /translation="MTMLRRCLALGFLALLLALGQPALAQVQDQGRPSNEELQLMQQL
                     QGQGPLTGRVSIPDQRAATLIQPAGKEWRDFWRGTSPWIGGIAIGGMLALLVLFFLIR
                     GRVPMEGGRSGETITRFNGLDRFAHWLTAISFLVLAITGLNIAFGRDLLLPLIGPEAF
                     SAFSDAGKVAHNFLSFPFTLGILLMLVLWVRDNIPSRLDLEWLAAGGGIIGHGHAHAR
                     RFNGGQKAIFWSVVLMGGAIAVTGYFLMFPFTVVADVGGMQTTQMVHSVLSMILVAII
                     LAHIYIGTIGMEGAFHAMGSGEVDLNWAEAHHDLWAEEIRARERRETGTAPGAMPAE"
     gene            10425..11849
                     /locus_tag="P5558_02860"
     CDS             10425..11849
                     /locus_tag="P5558_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRY23283.1"
                     /translation="MDAINAVIGFLNTIFWGYVLIYGLLAVGIFFTIRLGFIQFRHFG
                     EMFRVIRGSRETDAAGITPFQALTVSLASRVGTGNIAGVAVALYLGGPGAIFWMWMVA
                     LVGMATAYSESTLAQLFKVKNEEGQYRGGPAFYIARGLGAPWAAVIFSIALILSFGLV
                     FNAVQANSIAQAVEGAFNIPRWATGVAIAVVTGIVIFGGIRKIATVAEFVVPFMAGVY
                     LLLAVIVLIMNLGEVPGMLGHILASAFGFQEAAGGVTGGLAAAMLNGVKRGLFSNEAG
                     MGSAPNIAACAVPQPHHPSSQGFVQALGVFIDTLLVCTATALLILLSGVMGPENELTG
                     TALTQAALVEHIGVFGGPFIAIAIFFFAFTSIIGNYSYAENALTYLGAGNRTGITVLR
                     VIVLAMVVWGAMQTVATVFDAADASMGLMATINLIAIVLLSGTVVKLTQDYFAQRKAG
                     QEPVFHVADYPELTGEIDNEIWRR"
     gene            complement(11875..11997)
                     /locus_tag="P5558_02865"
     CDS             complement(11875..11997)
                     /locus_tag="P5558_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23284.1"
                     /translation="MRAFLASLVATVVIAVGAYYLIGDFQRSVQDAFATQSVRL"
     gene            complement(12065..12562)
                     /locus_tag="P5558_02870"
     CDS             complement(12065..12562)
                     /locus_tag="P5558_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HRY23285.1"
                     /translation="MTIELHPQPLTPEAFAPFGRVLEADPATARQINAGTTTRFHALA
                     TADPGPDGEVIISFFHGLPRPRPIAMALMERHPLGSQAFVPMQPHPWLVVVAERPEPE
                     ALRAFLLRGDQGVQYARGVWHHPLLVLAPSQSFVVVDRNGPGVNYEEVVLPLNAARLI
                     VPAGF"
     gene            complement(12559..13572)
                     /gene="alc"
                     /locus_tag="P5558_02875"
     CDS             complement(12559..13572)
                     /gene="alc"
                     /locus_tag="P5558_02875"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HRY23286.1"
                     /translation="MAEIVAVPAALPDWGEGLVNLASPRLGSRAVRCSDDSFAAMDRM
                     LQDGPAVFEPGRYDTYGKYMDGWETRRRRQGGHDWCVIRLGARGTIRGVDIDTSHFTG
                     NYPPAASLWASDAEAEPGDDVALWTEILPATSLGPSAQHYLEVEEPGPWRWLRLNIYP
                     DGGIARLRVLGQPAPDWSTAGDGPIELSALENGGRVIAYNDAHYGNVWALLSAGRGRD
                     MGDGWETRRRREPGHDWLILALGAAGTIERVEVDTAHYKGNYPDRCSLQASRVTFGTD
                     RSLVTQAMFWPALMSERPLGPDAIHVFEGKDLADLGPVTHVKLNIHPDGGVSRLRIMG
                     RRA"
     gene            13748..15985
                     /locus_tag="P5558_02880"
     CDS             13748..15985
                     /locus_tag="P5558_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HRY23287.1"
                     /translation="MSQVTVTVRAHAAAGSDFSKLRLLLDGKVVGEAVVRGSTPTDTV
                     FVLEDVDPTVAHHIGVEHYHDTTTRALTIESVTVAGTMIDASQGVQDVGPHDGRVLVA
                     GDDAGRIVYNGTWEVDVPASVFRAGPTPDPEPEPDPDPVPDPDPTPDPGPDPDPDPGP
                     TGEGGDTEIVLTLAAAPGSDFAKFRLLVDGALVGEARVTTASATELAFTVAGLDDGAP
                     HEVAVEHYHDTAARALSILGVAINGTALDPADGAQDVGPRDGKTIWNGEAATTITFNG
                     TWYVQVPAAVFGEAGPDPVPDPDPTPEPTPDPVPAGDYGALVAGLGGIAHWRFAETAG
                     SQAADALGASDASLRGGVALGAAGLVGDDGAVRLDGQDDYLLVSSSGEPIRLMILGDS
                     LSTATSPRMAASETLPGQLAPRLAEAGWNVELINLSKGGSTVVGGLDRLEAYLANGAS
                     PLPDAALVALGTNDSRSKLALDTVEARLHDIMDLLDQNEVATLLAGSRAQWPDGTHGY
                     QTTAQAEAFMQIFEGEAAEHGAGYYPEILKAVFYAGVDTYTSDGVHPTPAGNALILDD
                     LIDEIEALLAPFGKGDADSLALAQGTVSLWFAADDLNGKQGLLSKDASGYGGGGDVSL
                     YLDGDQLVLRLQDGSSSHVLSSADGAVTAGEAHHVAFTFGAAGAALWLDGAEVARSAY
                     TGGLESNGEPLVLGALAWTSARGTGDKITDAFDGRLDEVAIFGGQLTHEAVAMLAGLD
                     EPTVV"
     gene            16023..16445
                     /gene="arfB"
                     /locus_tag="P5558_02885"
     CDS             16023..16445
                     /gene="arfB"
                     /locus_tag="P5558_02885"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRY23288.1"
                     /translation="MLIVSDRIAIPENELDEAFVRASGPGGQNVNKVSSAVQLRFDVR
                     GSPSLPEAVKERLERLAGRRLSRDGVLVIDARRYRTQERNREDARERLAALVRAAEEL
                     PIARRATRPTRASRERRLVGKAVRSRVKTLRSRRPEPD"
     gene            complement(16446..17774)
                     /locus_tag="P5558_02890"
     CDS             complement(16446..17774)
                     /locus_tag="P5558_02890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRY23289.1"
                     /translation="MTTPPEEASLLEAATGLVTHILERLDLPIAVRLWDGRRVAAPGA
                     SSDVDEIRVAGPGTLRSLLRRPTLDTLFRLYVTGAIEPVDHDLLALMNRLQGAAKQHR
                     GRLRHVLAGFPWNAARKLAFAPTATNRVTHAYAGDAEARDSRSRDDKRLVQFHYDASN
                     DFYRLFLDAEMVYSCAYFETFETDLDTAQTAKLDLICKKLRLRPGERFLDIGCGWGAL
                     VCHAARHYGVIAHGVTLSQAQLDLARAKVQSLGLGDRATLALEDYRNLDGQWDKIASI
                     GMYEHVGIDNYPAYFRKLGKLLAPGGILLNHGITRRAKKEQKAFRRPSASRRIILRYI
                     FPGAELDHIGHTLEVMETAGLEVHDVEGLRRHYARTCRLWHERLMARREAAIDHVGAE
                     RFRMWGAYLAGVAIGFDRGPLRLFQVVATRQAADGTSPLPPTRADLYVDP"
     gene            complement(17794..18279)
                     /gene="mntR"
                     /locus_tag="P5558_02895"
     CDS             complement(17794..18279)
                     /gene="mntR"
                     /locus_tag="P5558_02895"
                     /inference="COORDINATES: protein motif:HMM:NF008273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HRY23290.1"
                     /translation="MTSSSVAKPATPEAADDGEPAVQGLVAMETHVEAFRHVRAARRN
                     ELVEDYVELIADLIDDGGEARQVDIAARLGVAQPTVAKMLKRLAEEGLIQQRPYRAVF
                     LTETGRELAEESRRRHQIVESFLLALGVSADIAQADAEGIEHHVSDETLDAFRRFAAD
                     R"
     gene            18506..19426
                     /locus_tag="P5558_02900"
     CDS             18506..19426
                     /locus_tag="P5558_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRY23291.1"
                     /translation="MTIGRGRRIILSSVAAGILLGAGAAGTGVAAEKLKAVTTFTVLA
                     DMAQNVAGDAAEVVSVTKPGAEIHGYEPTPQDIVRAHDADLILWNGLGLERWFEQFLA
                     NLGDVPAVVLSDGIEPVPIGEGAYEGRANPHAWMGLENALVYVDNIRDAFVAHDPDNA
                     ATYEANAAAYKASIRETIEPLRERIAAIPEDQRWLVTCEGAFSYLARDFGLHELYLWP
                     INADQVGTPQQVRKVIDAVRDHRIPVVFCESTVSTAPAEQVARETGAAYGGVLYVDSL
                     SEAGGPVPTYLDLLRVTAETVAKGLEGGGG"
     gene            19423..20349
                     /locus_tag="P5558_02905"
     CDS             19423..20349
                     /locus_tag="P5558_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese/iron ABC transporter ATP-binding
                     protein"
                     /protein_id="HRY23292.1"
                     /translation="MSEAVAQATAVEEPAPVGSEPGSGILGRDVTVTYRNGHTALRHA
                     SFAVPCSTITALVGVNGAGKSTLFKAIMGFVPIAAGEIRILGLSVREALARNLVAYVP
                     QAEEVDWAFPVLVEDVVMMGRYGHMGFFRRARPADRAAVTEALARVGMSDFRERQIGE
                     LSGGQRKRVFLARALAQDGRVILLDEPFSGVDVKTEEQIVALLKSLRDEGRVMLVATH
                     NLGSVPEFCDRTVLVKGTVLADGPTALVFTRENLERAFGGVLRHFTLGGEHLHDDDDP
                     RRISVITDDERPLVRYGGRTWRSGTNGKDRKL"
     gene            20346..21230
                     /locus_tag="P5558_02910"
     CDS             20346..21230
                     /locus_tag="P5558_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRY23293.1"
                     /translation="MIELLLQPFDYGYMTNAIWVSALVGGVCAFLSAYLMLKGWSLIG
                     DALSHSIVPGVAGAYMLGLPFALGAFVAGGLAAGAMLFLNQRSGLKVDVIIGLIFTSF
                     FGLGLFMVSINPMAVSIQTITMGNILAIDAADTLQLAIIGFVSLAVLLAKWKDLMVTF
                     FDESHARAIGLKPDLLKAVFFVLLAACTVAAMQTVGAFLVIAMVVTPGATAYLLADRF
                     ERLLVVAVTIGVSTGAVGAYASYFLDGATGGIIVALQTLIFLAAFALAPKHGLVAARR
                     RAAAALACDAGASPEGLP"
     gene            21227..22078
                     /locus_tag="P5558_02915"
     CDS             21227..22078
                     /locus_tag="P5558_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRY23294.1"
                     /translation="MSVADLLLPFQFPFMQNAFWIVLVISVPTALLSCFLVLKGWALM
                     GDAVSHAVLPGVVLAYVAGLPLIVGAFAAGMTCALVTGYLAHNSRVKQDTVMGVVFSG
                     MFAIGIILFTSIESDQHLDHILFGNMLGVGRADLLTSGAISAGVTLVLLAKWKDFLLH
                     AFDPAQARASGLKVAWLHYGLLAILSLTIVATLSSVGIILAVGLLIAPGAIAFLLARR
                     FGTMLVTAIAVTILATFLGTYLSFFLDSAPAPTIVLVLTGFFLVAFVRRLRLTRMASA
                     SVGAGEG"
     gene            complement(22110..22388)
                     /locus_tag="P5558_02920"
     CDS             complement(22110..22388)
                     /locus_tag="P5558_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23295.1"
                     /translation="MSLIPRQAATNEPAKSTIEPGFRTNEPAFSTNEPGRRPTNPAAG
                     KLLPDNVFPELPARPERAPGAQKPIMPNEPGTGLTAASASDNIPIIDT"
     gene            complement(22740..23417)
                     /locus_tag="P5558_02925"
     CDS             complement(22740..23417)
                     /locus_tag="P5558_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRY23296.1"
                     /translation="MAPPRRHRLSLLALLALLPGAGLAATTLTGRAEVVDGDTIRLGT
                     TVVRLADIDAPELGQRCDGPRELQHCGVVATNFLADRIAGETVTCAVAGLDDYGRSIA
                     TCSHEGQDLSAWLVAEGYALAFVRYSARLVPLQEEAEAKLAGLWQARLEPPWDYRARR
                     WQAAEQSAPNGCAIKGNISQSSGDRIYHTPWSPHYDRTRITESQGERWFCSEGEALAA
                     GWRPPRR"
     gene            complement(23557..24585)
                     /locus_tag="P5558_02930"
     CDS             complement(23557..24585)
                     /locus_tag="P5558_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRY23297.1"
                     /translation="MLASAAATGLLATGRPARAAAPGLRRAGAGLRWQGRPVHLYGPN
                     FNAFTGDLGPAPHVAEIATIAGLWGANCVRLLVAPAEYRERSPEAFWAHLLPILDACE
                     RQRLFVILNWWCIGWPGGASQDIDHWFRFTNYNDSSIPLWTRFWQDAATRLGDRGAIA
                     FSLFDEPTAHYNPPETRFDRLDQWYHVQPLYQDVIETIRATGATNLLSLSGGHWAKDF
                     SRWRERPIDDPAGNFLASFHMFADGEWADFDRLVGGLDQEIPFLCGGWGYLPTDLAEP
                     PDAGFTRWLETHAFPDGSFVGHCPWAHAIWSDPSALATGWPRPEPATLTAFGRYARAH
                     IPDGLVRP"
     gene            complement(24620..25720)
                     /locus_tag="P5558_02935"
     CDS             complement(24620..25720)
                     /locus_tag="P5558_02935"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY23298.1"
                     /translation="MKLLVPGCAGFIGSHFLERMLAREAVEIVGWDGQTDKIEHLLDH
                     PRLTLRRRLLKGPEAFVQFEKDLAACDWVVNLAAICNPSQYNTEPLRTIYANFIDGYQ
                     IVELAAKYKKPLIHYSTSEVYGRTLSSYLPGQPYDNPELYVLDAATTPMIMGPVQNQR
                     WTYATAKQLLERLVYAYHKEAGLRFAIVRPFNFFGPRMDYLPGIEGEGKPRVLAMFVG
                     AVLNDDPILLVDGGHAMRTITSIHDAIDALVAIIDRPEHSLDHFYNIGNPANEVSMRD
                     LAHGVRRAFARVSGDDRYLEHPIQEIGSKEFYGDGYEDCDRRVVDISAEQERLAWQPH
                     RPLDHVLDETVGYYWRKYGQRAPQRQPATAVG"
     gene            26193..27182
                     /locus_tag="P5558_02940"
     CDS             26193..27182
                     /locus_tag="P5558_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exostosin family protein"
                     /protein_id="HRY23299.1"
                     /translation="MIVSEDYQYFKEIFAHLKELAGRPGLRLALAVDTRDAVGEVDAD
                     TVAIVTSDENCQLPVNVRDAGLILKCHGVEPYPMPASPVLPAALVERLRVARERRRWR
                     ADAARLGPAGVAGLLAKTELVPMGYCKQLDLPLVPFAERRRVFSFRGSVDNYRAGLLS
                     RHAVTNYPKRVARQRFLAALREVAREAPADACDLRITTDFDDSLKDAGRDYSTALMDS
                     RFCLCPRGTRLETFRIFEGLRYGCITVTEALPDRWYLRGAPLLSVGDWRELPGLMRRL
                     LADPERMAELHHRSLAWWRDMISPLAIARRVLPRLERGGPARHAAVGSAGGLG"
     gene            27182..>29009
                     /locus_tag="P5558_02945"
     CDS             27182..>29009
                     /locus_tag="P5558_02945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY23300.1"
                     /translation="MVLALFVLAALALFLGLYSFVFYAPSLALLPARPVARRDVADGR
                     RPRITLCFCAYREEQVVPLKLQNLRLLRERHPDLEILAYVDASPDRTAELLEAAPELL
                     TVIVGEERRGKSHGMNRLVAAATGDIVVFTDANVLLKDDALDVIAAHFADPEVGCVCG
                     RLLYVDDHNPAAHVNSLMWRLEERIKRAESRTGSVIGADGSLFAIRRALHRPPPADII
                     DDFYVSLSILLDGHRVISAPDAIAYENSVHDPYEEMQRKVRIACQAWNVHRLLWPRLR
                     RLDAVHLYKYVAHKLLRWFSPFALALAVLLVSTALLLALPLTLFLQLLVAGPLALALG
                     LWLRLGAAEATHSTVLSLLGVGIGITRSLRGERFQTWTPATSARQRLERPRLVYFSHD
                     RNDANVWQRCQDFTALGFVLTNVSWHRDKNSQDGERPWHEIDLGRTVDAAYWHRAWSV
                     VRGAFGVLCHFQQLRDADLVVARNFEPSVLAMLARRLFNRRVPFAYECVDIHRFLTSR
                     SVLGDACRLAERRVLAAADLLIVSSPDFASRYFAPVHGYDGPVYLLENKMPSFLARRF
                     AAPTMLRRPHVGPPWRIGWFGMLRDMESLGLLLRLAAMRPELV"
     assembly_gap    29010..29019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29020..29650
                     /locus_tag="P5558_02950"
     CDS             <29020..29650
                     /locus_tag="P5558_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23301.1"
                     /translation="YGLTETSSTIALLGSDDHREAFASDDPAVRGRLASVGRPLPSVE
                     VSIRGYPTEITENELRQMVEPYPNVTYGGPYQKATELRSIYENVDLVWAIDLYDAGVN
                     SDWLLPNRLYEAGFFGRPVIARAETAIGRFVADLQMGCVLSPPYDVACLDLLGQLSGD
                     HYAAIVRWMEELPRQRFVLDEELREMTQNLLSPQAAGAVVAELRNSNVA"
     gene            29643..>30069
                     /locus_tag="P5558_02955"
     CDS             29643..>30069
                     /locus_tag="P5558_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRY23302.1"
                     /translation="MPDRPEAAVPKARAARLEHIDSLRGVACLLVVYIHAVQWLERNA
                     FPMSGPEATLLHASITWLDPGKVGVLVFFAISGYVVPFSLRRPLAHPLREFVVNRFFR
                     LMPAYWFSLVAGVLIIIWFEQRPTDLAGWLGNLILAPQLL"
BASE COUNT         4290 a        10061 c        10968 g         4740 t
ORIGIN      
        1 caggccgagc ttgtcgccca tgggcaggaa gctcgccggc gtggcggtgc cgggccggcg
       61 ctcgagctgc cagagcgcct cgcccaggct gtcggggtcg tcggcctcga tgagctggac
      121 gcggcccgtg ccgtagccga ggccggcgag gctggcctcg gcgaggccca tgacacgggc
      181 gagcggggtg aggtcgggcc ggcgccggcc ggtcgcaagg atgcggatgt cggcggcccc
      241 catggccatg gcaccggcga ggagctcgat gccgagctgg gtgatctcgt tgaccgccac
      301 cgggatcacc cgggccggca ggccgtcgcc gacgcgggcg aggaggtcga tcagggcggc
      361 gccgtgctcg gcatcgtgga gcagcagcac ggcatccgtg ccgccggcgg cgtggtaggt
      421 ggtgaggagg gtgcggacgc gttcgagcag gtgctcggcg ggcgggtagg cataggacgc
      481 ggcgccggtc gggcagacgg cgtggcagcc gccgcagccg gcgcagatgg cggcgtcgat
      541 ggcgacgtgg tcgccgttgg gggtgatggc gccggtcggg cagacctcga ggcagcggct
      601 gcagccgacc gtgcggttgc ggctgtgggc gcagagctcc ggccggtagg cgacgtggcg
      661 gggcttgtcg aaggtgccga cgaggtcggt ggcggccagg agcgccttct gcacggcgac
      721 cgggtcggcg gggtcggcat ggagatagcc gggtcgcgtt cggggcgagg ggaagagcgg
      781 ggtgccgccg gtgaggtcga ggatgatgtc gcaattggag accgcctcgt tgcgggccgg
      841 gccgaagcgg agccggcggc gggacgaggg ggcgggcagg gcgtagtcgt cgacgacgag
      901 ctcgaaggcg ccgagatggc ccctggccgt gcgaatggtg ccctggagga cggggaaggt
      961 ggtccgggcg gggggcgtga catctcccgg gcgggtcaag aggacggtga cgtcgaggcg
     1021 gtcgccgagc tgggcggccg cttcgatcgc cacctcgtcc cggccgtaga cgagggtgac
     1081 gccgccggat tccagggtga ccacatgggt ctcgggcacg ggcagggcgg cttcggcgag
     1141 aagggccgcg atcttcggcg tggcttctgc cgcctccttc gaccaggcgg cgcgggcgcg
     1201 gatgtcgacg gtgaccaggc tttcggccgc ggcgtggccg gcggcctcgg tcagggcctc
     1261 ttcgaagacc gcgcgttcct gggcgcaggc gacgatgccg ggcgcctcgc ccgcgaggcg
     1321 tgcctgctgg cggcggcaga ggctggtggc gatggcgggc gtttcggtgc cgagcgcgcg
     1381 tgccagcgct tcggcgtcga gcggcatggt ggcctcgcaa tcgcagagca tgacccgctt
     1441 ctcgcccacc cgcacaagca tctcccgtat cgatccagag ccgaacgcac tacatgaacg
     1501 ctatgcgcat ggccgacaat cctcgaacgc tgccggacct gccgccgccc ttccgggtgc
     1561 ggtgggtggc gggcgacgtc atggcggcgg cgcggcaggc ggcggaacgg ggcgaggagc
     1621 ccggcctgct gctgatcggc gaggacgaag cgacctgtga tctggccctg gcgctcgccc
     1681 ccgaccggcc ggaggcggcc tgccgggtgg tcctgcatct ggcgatgctg gccgtggcgg
     1741 atgcgctggc cgccgtcggg ccgcccttga aggaggtggt gttcgcctcg cccgacgggc
     1801 ttcggctcga cggggccaag gtcgggcggg tgcggctggc gatggcggag gtggcggcgc
     1861 cggacgaggt gccggaatgg tgcgtcgtcg gccccgaggt tcgcatgatg ctgccgccgg
     1921 cggccggcga gccgggtgcc atgccggagg tcacggcgct ccaggaggag ggcttcggcg
     1981 gtgtcggcgt cgtggagctg gcccagagct tcgcccggca tttcctctac tggatgcatc
     2041 gctggaccga ggagggcagc ggcccggtgc tggcgcactg gcgggcacgg ctggaccccc
     2101 tgacgatcga gccggtccta tgaccctgaa gctgccccgg accatccggc tcgatgcctc
     2161 ggacgaggcg gtcttcgagc gggcggccga gccgggcgag tgggccgtgg cgggcggctt
     2221 cgcgttcctg gacacggacc cgacagcgct cgccggcaag cggcggcaag ccttcgtctc
     2281 gggctttctc gggaccgagt cgcgcggctg gtcgaccttc gtcgtggtgg ccacggccga
     2341 cgcggcggcc gaggccgaga tggtcgagcg gctggcccgg ctcttcgtcg cggcgcacgg
     2401 ggcccccgat ctcgacgccg cccgcccggt ggcgcgccag gaggtcgagc tcatggccgg
     2461 actctgccgc gaccatgcgc cgggcacgct cttcgccatc acccgcagcg tcgatgcgga
     2521 gggcggcatt cgcgagagct ttcgggcggt cgaccgcaag gccggggtgc atggccgggt
     2581 ctgggatgtc gtgccggacg agggccaggg atgagcgggg cgatctgggc cagcgccggg
     2641 cgcgagctgc tcgatccggc ggcaggcggc gggctcgccg tcacggcgag cttcctcaag
     2701 gcttatctcg cccggccgga gctggcgccg gtggcggagt cctgcacggc ggagcgggcc
     2761 ctgcacgcac ggctgctggc cgacccgttc gcggccgtgg aggatgcggc gctggcggat
     2821 ctcgccgacg cggatgccgc ccagaactgg cgggtctggc tgggattccg cgattttctc
     2881 gcccgtcggc cgagcctcga ggcggcctac ctggcgctcg tcaagggcga ggggccggcc
     2941 gtgcccgcgc tcttcgtcga ccagctcgtc catgtcatcc tgcgccacct cctcgacggc
     3001 gaagcggatg cctatcggtg gcgggcagcc gagtgcctgt tccggccgca gaaggtgagc
     3061 atcaccgagg gcggcatcct gctcgccgac gaggagacgg tggagcaggc ggcggcgacg
     3121 ggcgggttcg gcgggctcgg gcagttgctc aggcaggccg gcacggtgcc gcggagcgtc
     3181 gagctggacg tgctgggcga gggcaatgcc gcgagctacg ccggccggag cgagcgcttc
     3241 gacatggtgc tggacgtggc cttcacccgg ccgggctcgg atgccctggc ccgggtgctg
     3301 gagcgctggg tggcgcattt cctcagcctc gccatccggc tgcagccggt gcagaccatc
     3361 cgggacgaga gctgggcctg gcatgtcggg ctcgacgtcg aggcgacggg catcctcaat
     3421 gccatgtggg agggggtgga ggtgccgcag gccgacctgg cgcgcattct cgggctcttt
     3481 cggttggagt tcctcgatcc ctcggccatg ctggagcggg tggccgggcg gccggtctat
     3541 ctcgccatgg cgatggattg cgagaagcgg ctgcgcctca agccgcagaa tttgctgctc
     3601 gggctgccgt tgcggcagga ccgggcgagc tgagggggca tggcgactga tcggatgagc
     3661 gtggcggtga tcgtcgagcg gcggccggcc ggctcgcgct gggcgagcca tgtctggcgg
     3721 gcggtcgagg tgctgcccgg cgcaccggcg acgccggcct ggacggtgct ggccgagggc
     3781 gggggggcga cgcgcttttt cgccggcgtg gccgagctgg tcgtccattc gggcgagacg
     3841 gagccgctgg cgcacaatct gggggcggcc gtgccgagcg tgtgggtggt gctgcgccgc
     3901 gccgccaccc cgccgggctg gcggctgctg ctggccacgg tcgatgcggg cgaggcgcat
     3961 gcccatgcgg ataccggcga cgatctggtc gagatggtgg cgatgccgga tgcggtgcgc
     4021 gcctggctcg agcctttcgt tgcccggcat catgtcgagc ggcagcgcca caagcggcgg
     4081 cgcgaccggg ccgatccgga ggcgctggcg gtcggccggc gcgggggggg cgaggatggc
     4141 tgaggaggag cgcccgttcc tcgcccgctg gtccgagcgc aagcggcagg cgcaggcgcg
     4201 ggaggcgccg agcgaggctg tgccgcttcc ggtcgagtcc gaggcggcgg agccggagcc
     4261 ggatttcgac ccggcgagcc tgccgccggt cgagagcctg gggccgggca gcgacatcgc
     4321 ggccttcctc cggaagggtg tgcccgcggc gctgcgccgg gcggcgctgc ggcggatctg
     4381 gacggtcgac ccgaagatca agggctttcg cgaggtcgcc gactacgact gggacttcaa
     4441 cgcgccgggc tacggggcaa tctcgccgac cgatgatgcg aggcaggcgg ccgagcggct
     4501 gctcgcccgg ctgcgccggc cggcggccga gcctgcttca gcggaagatc ctgaggcggc
     4561 gccgacggag catgtcgtgg ccgaggaggg cgagcggccg gcggcgctgc cggtggagcc
     4621 ggaggcctcc gggccggagt gggagccgga accggtggcg cggcgacgcc atggcggggc
     4681 cacgccgcgc tgacgctgca cgtttcgcgc ttgtcactgc ggaaagcggg tctagacttc
     4741 aaaagcagaa tagcttcagg ttgcctggcc ggcggatgac cggcggggcg gccggtcggg
     4801 aggtcgatga gcgagccggg caacatggcg aacggggcgg caggcgacgt cgagctggca
     4861 cgggcgcggc tgtggcagct gctcggcctt tgcctgcgcg ctgcacccga tgcggcgacg
     4921 ctgcggctga tcgccggcct ccagggcgac ccgtcgcctc tggggtcgac gctcggcgag
     4981 ctggcgggtc tcgcccgttc cgccgacccg gtgctgctgg cgcgcgagca ccacgacctc
     5041 ttcatcggtc tcgcccgcgg cgagctcgtc ccctacgcct cctactatct gacgggtttt
     5101 ctgcacgaga ggccgctggc gcgcctgcgt ggcgacatgg ccgggctcgg catcgcccgc
     5161 acgccgggcc tggccgagcc cgaggaccac atcgcgtccc tggcggagag catggccggg
     5221 ctgatcgccg gcgacttcgc cgccgacgcg gccgcacagc agcgcttctt cgagcgccac
     5281 atcgccccct gggccggacg cttcttcgcc gacctggagg ttgcccaggc cgcccggttc
     5341 taccggccgg tcggcgcgct cggccggcac ctgatcgaga tcgagacgac agcctacgcg
     5401 ctggcggcgt gatgccggac cggcgcaacc gagggagacg acccatggcc aagagccctg
     5461 ccaacaccga agccccggcc tcgccggccc ggcgcggctt cttcaaggcg gcggccggcg
     5521 gtgccgcggg ggccgcgctg gtcgtcccgg cggtgctcgg tggcggggcc cgggcggcgg
     5581 aatcgggcga ggagcagcgc aagacgcgct accaggaaac cgaccacgtc aagacctact
     5641 accagaccaa ccggtactga cggggaggac gagggccatg ttgcagaagc gtcgtgccgg
     5701 ggtatcgagc aagaccggtc tggccggggt gctggctgcg gccggctcgg cgaccgtcga
     5761 tcgccgtgcg ttcctgcgga agtcgggtgt cgccgctggc ggcctcgccg ccctgggtgc
     5821 cgtgccgctc gccacggtca ggaaggccga ggccgggccg atgcagcccg gcgtcccgat
     5881 cgagatcaag aagaacatct gcacccactg ctcggtcggc tgcacggtca ccgccgaggt
     5941 gcagaacggg gtctgggtcg ggcaggagcc ggcctgggcg agccccatca atcgcggcac
     6001 gcattgcgcc aagggtgcgt cggtgcgcga gatcgcgcac ggcgatcgcc ggctgaaata
     6061 cccgatgaag ctctccggcg gcgagtggca gcggctgagc tgggagcagg cgatcgacga
     6121 gatcggcgcc aagctcgagg agctgcgcga ggaatccggg ccggacagcg tcttctggct
     6181 ggggtcggcg aaattctcga acgagggctc ctatctcttc cgcaagctcg ccgcgatgtg
     6241 gggcaccaac tcggtcgacc accaggcgcg gatctgccac tcgaccacgg tcgcgggtgt
     6301 cgccaacacc tggggctacg gggcgatgac caattcctac aacgacatcg ccaattccaa
     6361 ggcgatcatg atcatcggcg gcaacccggc cgaggcgcac cccgtctcgc tgcagcacgt
     6421 gctgcgcggc aaggagctga accaggcgcc gatgatcgtc tgcgacccgc gcttcacgcg
     6481 gacggcggcg catgccacgc actacgtgcg gctgcggccg ggcaccgaca ttcccctggt
     6541 ttggggcatg ctctggcaca tcttcgagaa cggcctcgag gacaaggagt acatcgccca
     6601 gcgggtccac ggcatggacc agatccgggc cgaggtggcg aagtggcatc cggccgaggt
     6661 cgagcgggtg accggcgtgc cgggagcgca ggtcgcggag atcgcccgga tcatggcgac
     6721 gcagaagccg tcgaccctga tctggtgcat gggcgtcacc cagcacacgg tcggcacggc
     6781 caatgtgcgc gccctctcga tcctgcagct ggcactcggc aatatcggca tcgagggcgg
     6841 cggggccaac atcttccgcg gccattgcaa cgtccagggt gcgaccgatc tcggcctcga
     6901 tgtgacctcc ttgccggcct attacgggct gtcggagcgg gcctggcggc actggtccag
     6961 ggtctgggac ctcgattacg agtgggtccg cgaccgcttc ggcaaggccg gcatgacggt
     7021 cgaggacaag cagaagctga tggaggccaa gggcattccc accacccgct ggttcgacgc
     7081 ggtcctggcg gatccggccg acgtggacca gcccgatcgg ctccggggca tggtggtctt
     7141 cggccatggc ggcaacacgg tgccgcgcat gccggagatg cgcgccgggc tcgaggcgct
     7201 cgacctgctg gtcgtggcgg atccgcaccc caccaccttc gccgcggtct ccgagcgcaa
     7261 ggacggcacc tatctcctgc cgatctgcac acagttcgag tgcaatggct cgcgcaccgc
     7321 gtcgaaccgc tcgctccagt ggggcaacca gatcgtcgag ccgctgttcg aatcgaagaa
     7381 cgactacgag gcgatgtacc tgctctcgaa gcggctcggc atcgaggagc agatgttcaa
     7441 gcatatccag gtggacggca cggcaccgct cgccgaggac atcctgcgcg agatcaaccg
     7501 cggcacctgg tcgatcggct ataccggcca gtcgccggag cggctcaagc agcacatgga
     7561 gcaccaaggc gacttcgaca tggtgagcct gcgcggcaag cccggcacac cggtcgaggg
     7621 cgaagtctac ggcctgccct ggccgtgctt cggcacgccg gagatcaagc atccgggcac
     7681 gcacatcctc tacaacacgc acgagcacgt gatgaacggc ggcggcacgt tccgcgcccg
     7741 cttcggcgtc gagcgcgacg gcgagacgct gctcgccgag gggagctggt ccaagggctc
     7801 ggagatcgag gacggctatc ccgagttcac ctacggcatc ctcaagacgc tggggtggga
     7861 cggcgatctc acggccgagg agctggcgac catccagaag atcggcggcg acgacccgga
     7921 cagcgtctcc tgggccatcg atctctcggg cggcatccag cgggtctcga tggcgcatgg
     7981 ctgcgtgccc tacggcaacg ccaaggcccg ggccgtggcc tggaacctgc cggacccggt
     8041 gccggtgcat cgcgagccga tctacagccc caggccggag ctggtcgccg agtaccagac
     8101 ctatgaggac aagcgcgatt tccgcgtcat gaatatcggc cacacgatcc aggcgcaggc
     8161 ggtgaaggag gggacggccg gctcctatcc gatcatcctg accaccggtc ggctggtcga
     8221 gtacgagggc ggcggcgagg agacccggtc gaacaagtgg ctggccgagc tgcagcagtc
     8281 gatgttcgtc gaggtcaacc gggccgacgc ggccaggctc ggcgtcaagg acggcggcat
     8341 ggtctgggtc tacggcccgg cggagggcaa ggtccgggtc aaggccttgg tcaccaaccg
     8401 ggtcggcgag ggggtggcct tcatgccctt ccacttttcc gggtattggg aaggtgagag
     8461 ccagcgggcc aagtatcccg aaggcgcgga cccgatcgtg ctcggcgaca gcgccaatgc
     8521 ctgcacgacc tatggctatg acccggtgac cgcgatgcac gagggcaagg ccacgctctg
     8581 ccgcatcgag gcggcctgag cggcggcgag agggagagaa gaacgatggc ccgcatgaaa
     8641 ttcctctgcg atgccgagcg ctgcatcgag tgcaatgcct gcgtcaccgc ctgcaagaac
     8701 gagaacgagg tgccctgggg catcaaccgg cgtcgcgtgg tgacgctcaa tgacggcgag
     8761 atgggcaagg agcgctcgat ctcgatggcc tgcatgcact gcaccgacgc gccctgcgcc
     8821 gcggtctgcc cggtggactg cttctacacg acggccgatg cggtggtgct gcactccaag
     8881 gagctctgca tcggctgcgg ctactgcttc tacgcctgcc cgttcggcgc accccaatac
     8941 ccgcagctcg gcaatttcgg cggccgcggc aagatggaca agtgcacctt ctgcgccggc
     9001 ggccccgagg cggacaacac gccggcggag tacgcgcgct acggcgccaa ccggctggcc
     9061 gagggcaagc tgccgctctg cgccgagatg tgctcgacca aggcgctgct cgccggcgac
     9121 ggcgagatca tcgccgccat ctacaaggaa cgggtggtga cccggggcta cggctcgggt
     9181 gcctggggct ggtcgacggc ctatcccgac agccagggcg tgtgagatgg cgagggagat
     9241 cggtgtcatg accatgctcc ggcgctgcct ggcgctcggc tttctggccc tgctgctggc
     9301 cctgggccag ccggccctgg cgcaggtcca ggaccagggc cgcccatcga acgaagagct
     9361 gcagctcatg cagcagcttc aggggcaggg gccgctgacc ggcagggtca gcattccgga
     9421 ccagcgggct gccaccctga tccagcccgc cggcaaggag tggcgcgatt tctggcgcgg
     9481 cacctcgccc tggatcggtg gcatcgcgat cggcggcatg ctggcgctgc tggtgctctt
     9541 cttcctgatt cgcggccggg tgccgatgga gggcgggcgg tccggcgaga ccatcacgcg
     9601 cttcaacggg ctcgaccgct tcgcccattg gttgacggcg atcagcttcc tcgtgctcgc
     9661 gatcacgggg ctgaatatcg ccttcggccg cgacctcctc ctgccgctga tcggccccga
     9721 ggctttctcc gccttctcgg acgcgggcaa ggtcgcccac aattttctct ccttcccgtt
     9781 cacgctcggc atcctcctga tgctcgtgct ctgggtcagg gacaacatcc cgagccggct
     9841 cgatctggag tggctggccg ccggcggcgg catcatcggc cacggccatg cccatgcccg
     9901 gcggttcaat ggcgggcaga aggcgatctt ctggtcggtc gtgctgatgg gtggggcaat
     9961 cgccgtcacc ggctatttcc tgatgttccc gttcaccgtg gtcgccgatg tcggcgggat
    10021 gcagaccacg cagatggtgc attcggtcct gtcgatgatc ctcgtcgcca tcatcctggc
    10081 gcacatctat atcggcacga tcggcatgga gggggcgttc catgcgatgg gctcgggcga
    10141 ggtcgacctc aactgggccg aggcgcatca cgatctctgg gcggaggaga ttcgggcgcg
    10201 cgagcgccgc gagacgggga ccgcgccagg ggccatgccg gccgagtagc gccgcgggcg
    10261 gctgctcccg ctgtgtccga cctgccgcgt caaaccataa tttcaacagc ccggcttgcg
    10321 ccaatcgtcg cgtcgccgtc atagtcgccg cggggcccac accgcatcgc gagccgagtc
    10381 ggcaaaaaga cggtgcgccg cgcctcactg gcagggaggc cgtcatggac gcgatcaacg
    10441 ccgtcatcgg atttctgaac actattttct ggggctatgt gctgatctac ggcctcctgg
    10501 ccgtgggcat cttcttcacg atccggctcg gcttcatcca gttccggcat ttcggcgaga
    10561 tgttccgcgt catccgcggc tcgcgcgaga cggatgcggc gggcatcacg ccctttcagg
    10621 cgctgaccgt cagcctcgcg tcgcgggtcg gcacgggcaa catcgccggc gtcgccgtgg
    10681 ccctctatct cggtggaccg ggtgccatct tctggatgtg gatggtcgcc ctggtcggca
    10741 tggcgaccgc ctattccgag agcacgctcg cccagctctt caaggtgaag aacgaggagg
    10801 gccagtatcg cggcggcccg gccttctaca tcgcccgcgg gctcggggca ccctgggccg
    10861 cggtgatctt ctcgatcgcg ctgatcctct ccttcggcct cgtcttcaac gcggtgcagg
    10921 ccaattcgat cgcccaggcg gtcgagggcg ccttcaacat cccgcgctgg gccaccgggg
    10981 tggccatcgc cgtcgtgacc ggcatcgtca tcttcggcgg catccggaag atcgcgaccg
    11041 tggccgagtt cgtcgtgccg ttcatggcgg gcgtctacct cctgctcgcg gtgatcgtgc
    11101 tgatcatgaa cctcggcgag gtgccgggca tgctggggca tatcctggcc agcgccttcg
    11161 gcttccagga ggcagccggc ggggtcacgg gcggcctggc ggcggccatg ctcaacggcg
    11221 tcaagcgggg gctcttctcc aacgaggccg gcatgggctc cgcccccaac atcgcggcct
    11281 gcgccgtgcc gcagccgcat cacccctcgt cgcagggctt cgtccaggcg ctgggcgtgt
    11341 tcatcgacac gctcctggtc tgcaccgcga cggcgctcct gatcctgctt tccggcgtca
    11401 tggggccgga gaacgagctt accggcacgg cgctcacgca ggcggcgctg gtcgagcata
    11461 tcggcgtctt cggcggcccc ttcatcgcca tcgccatctt cttcttcgcc ttcacctcga
    11521 tcatcggcaa ctactcctat gccgagaacg ccctcaccta tctcggtgcc ggcaaccgga
    11581 ccggcatcac cgtgctcaga gtgatcgtgc tggcgatggt ggtctggggg gccatgcaga
    11641 cggtggcgac cgtgttcgat gccgccgatg cctcgatggg cctgatggcg accattaatt
    11701 tgatcgccat cgtccttctc tcggggacgg tggtgaagct cacccaggac tacttcgcgc
    11761 agcgcaaggc cgggcaggag ccggtcttcc acgtcgccga ctaccccgag ctgaccggcg
    11821 agatcgacaa cgagatctgg cggcgttgag gcgggcctcg agagcgtccg gctgctagag
    11881 ccggacgctc tgggtggcga aggcgtcctg gaccgagcgc tggaagtcgc cgatcaggta
    11941 gtaggcgccg accgcgatga ccacggtggc gaccaggctc gcgagaaatg cccgcatcgt
    12001 ttcttctccc acaggggggc cggtcggtcc ggcgcccttt tttctagcaa caaaagtggc
    12061 ggcatcaaaa cccggcgggg acgatcagcc gggcggcgtt caggggcagc accacctcct
    12121 cgtagttgac ccccggcccg ttgcggtcga cgacgacaaa gctctgggac ggcgccagga
    12181 cgaggagcgg gtggtgccag accccacggg cgtactgcac gccctggtcg ccgcgcagga
    12241 ggaaggcgcg aagggcctcc ggctcgggcc gctcggcgac cacgacgagc caggggtggg
    12301 gctgcatcgg cacgaaggcc tggctgccga gcgggtggcg ctccatgagc gccatggcga
    12361 tcggccgggg gcgcggcagg ccgtggaaga aggagatgat gacctcgcca tccggcccgg
    12421 ggtcggccgt cgccagcgcg tggaagcggg tggtggtgcc ggcgttgatc tggcgcgcgg
    12481 tcgcggggtc ggcctcgagc acccggccga agggggcgaa ggcttccggc gtgagcggct
    12541 ggggatggag ctcgatggtc atgcccggcg gcccatgatg cgcagccggc tgacgccgcc
    12601 atcggggtgg atgttgagct tgacgtgggt gacggggccg aggtcggcga ggtccttgcc
    12661 ctcgaagacg tggatcgcgt cggggccgag cgggcgctcg ctcatgagcg ccggccagaa
    12721 catcgcctgg gtgacgagcg aacggtcggt gccgaaggtg acgcgcgatg cctggagcga
    12781 gcagcggtcg gggtagttgc ccttgtagtg ggcggtgtcg acctcgacgc gctcgatcgt
    12841 gccggcagca ccgagcgcga ggatcagcca gtcgtggccg ggctcgcgcc gccgcctggt
    12901 ctcccagcca tcgcccatgt cgcggccgcg gccggcgctg agcagcgccc agacattgcc
    12961 gtaatgggcg tcgttgtagg cgatcacccg gccgccattc tccagcgccg agagctcgat
    13021 cgggccgtcg cccgcggtgc tccagtcggg ggcgggctgg ccgaggacgc gcagccttgc
    13081 gatcccgcca tcggggtaga tgttgagccg gagccagcgc cagggaccgg gctcctcgac
    13141 ctcgaggtag tgctgggcgg acggcccgag gctggtggcc ggcaggatct cggtccagag
    13201 tgccacatcg tcgcccggct cggcctcggc gtcgctggcc cagagcgagg ccgcgggcgg
    13261 gtagttgccg gtgaagtggc tggtgtcgat atcgacgccg cgaatggtgc cgcgggcacc
    13321 gaggcggatg acacaccagt catggccgcc ctggcgccgg cgtctggttt cccagccgtc
    13381 catgtacttg ccgtaggtgt cgtagcggcc gggctcgaag acggcggggc cgtcctgcag
    13441 catgcggtcc atggcggcga agctgtcgtc cgagcagcgg actgcccggc tgccgaggcg
    13501 cggcgaggcg aggttgacga gaccttcccc ccagtccggc agcgctgccg gcacggcgac
    13561 gatttccgcc attttttttc cctgatgagg tgtcctgaaa aggtggcggg cgccatgcta
    13621 gcgccgtccg acgctgccgt ctgccttcgc gagtatgtcc gtgcatgccg ggcgtcatga
    13681 tgaacattgc attaaccatt caccaaatgc tcgattaagg gtcgcattga tcaaccggat
    13741 ttacgccatg agccaggtca cggtcaccgt tcgcgcccat gccgccgccg gcagcgattt
    13801 cagcaagctg cgtctcctgc tcgacggcaa ggtggtcggc gaggcggtgg tgcggggctc
    13861 gacgccgacg gacacggtct tcgtgctcga ggacgtcgat ccgacggtgg cgcaccacat
    13921 cggcgtcgag cactatcacg acaccacgac gcgcgccctg accatcgaga gcgtcaccgt
    13981 cgccggcacg atgatcgatg ccagccaggg cgtgcaggac gtggggccgc atgacggccg
    14041 ggtcctggtg gccggggatg acgccgggcg gatcgtctac aacggcacct gggaggtcga
    14101 cgtgcccgcg agcgtcttcc gcgcgggccc gacgcccgat cccgagccgg agccggaccc
    14161 cgatccggtg cctgatccag accccacgcc agacccaggc cccgaccctg accccgatcc
    14221 tggcccgacg ggtgagggcg gcgacaccga gatcgtgctg acactggccg cggcaccggg
    14281 ctcggacttc gccaagttca ggctgctggt ggacggcgcg ctggtcggcg aggcgcgggt
    14341 gacgaccgcc tcggcgacgg agctcgcctt cacggtcgcc gggctcgatg acggggcacc
    14401 acacgaggtg gcggtcgagc actatcacga cacggccgcc cgggcgctca gcattctcgg
    14461 cgttgccatc aacggcacgg cgctcgatcc ggcggacggc gcgcaggacg tggggccgcg
    14521 cgatggcaag acgatctgga acggcgaggc ggcgacgacg atcaccttta acggcacgtg
    14581 gtatgtccag gtgccggccg ccgtcttcgg cgaggcgggg ccggacccgg tccccgaccc
    14641 cgacccgacg ccggagccga cccccgaccc ggtgccggcc ggcgactacg gcgccctggt
    14701 cgccgggctc ggcggcatcg cgcactggcg cttcgccgag acggcgggga gccaggcggc
    14761 ggatgcgctc ggggcgagcg acgccagcct gcgcggcggc gtcgccctgg gtgcagcggg
    14821 cctcgtcggc gacgatggtg ccgtacgact cgacgggcag gacgactatc tcctcgtgtc
    14881 gagcagcggc gagccgatcc ggctgatgat cctgggcgac agcctgtcga cggcgacctc
    14941 gccgcgcatg gcggcgagcg agacgctgcc gggccagctc gcaccgcggc tcgccgaggc
    15001 cggctggaac gtcgagctga tcaacctctc gaagggcggc agcacggtgg tcggcgggct
    15061 cgaccggctc gaggcctatc tcgccaacgg ggccagcccg ctgccggacg cagcactcgt
    15121 ggccctcggc accaatgaca gccgcagcaa gctcgcgctc gacacggtcg aggccaggct
    15181 gcacgacatc atggacctgc tcgaccagaa cgaggtggcg acgctcctgg ccggcagccg
    15241 ggcgcagtgg ccggatggca cgcacggcta ccagacgacg gcccaggccg aggccttcat
    15301 gcagatcttc gagggcgagg ccgccgagca cggtgcgggc tactaccccg agatcctgaa
    15361 ggcggtcttc tacgcgggcg tcgataccta caccagcgac ggcgtccacc cgacacccgc
    15421 gggcaacgcg ctgatcctcg acgacctgat cgacgagatc gaggcgctgc tggcgccctt
    15481 cggcaagggc gacgccgatt cgctggcgct cgcccagggc acggtctcgc tctggttcgc
    15541 ggccgatgac ctgaacggca agcaggggct cctgagcaag gacgcatcgg gctatggcgg
    15601 gggtggcgat gtcagcctct atctcgacgg cgaccagctc gtgctccggc tgcaggatgg
    15661 cagcagcagc catgtgctga gctcggcgga cggggcggtc accgccggcg aggcgcatca
    15721 tgtggccttc accttcggtg cggccggggc ggcgctctgg ctcgacgggg ccgaggtggc
    15781 gcgctcggcc tatacgggcg ggctcgagag caatggcgag ccgctggtgc tgggggcgct
    15841 cgcctggacc agcgcccgcg gcaccggcga caagatcacc gacgcgttcg acggccggct
    15901 cgacgaggtg gcgatcttcg gcggccagtt gacgcacgag gcggtggcga tgctcgccgg
    15961 gctggacgag ccgacggtgg tgtagctttc cgcaacccgg tcagtctcct aggatgggtg
    16021 ccatgctcat cgtcagcgac cggatcgcca ttcccgagaa cgagctcgac gaggcgttcg
    16081 tgcgcgcctc ggggccgggc gggcagaacg tcaacaaggt gtcgagcgcc gtccagctcc
    16141 gcttcgacgt gcgcggctcg ccgagcctgc cggaggcggt gaaggagcgg ctcgagcggc
    16201 tggccgggcg gcggctgagc cgcgatggcg tgctggtgat cgacgccagg cgctatcgca
    16261 cccaggagcg caatcgcgag gatgcgcggg agcggctggc ggcgctggtc cgggcggccg
    16321 aggagctgcc gatcgcccgg cgtgccaccc gcccgacccg ggcgagccgc gagcggcggc
    16381 tggtgggcaa ggcggtgcgc tcgcgcgtca agacgctgcg gtcgaggcgg ccggagccgg
    16441 actagtcagg ggtcgacgta gagatcggca cgggtcggcg gcaaaggtga ggtgccgtcc
    16501 gccgcctggc gggtggcgac gacctggaag agccggagcg ggccgcgatc gaagccgatg
    16561 gcgacgccgg cgagataggc cccccacatc cggaagcgct cggcgccgac atggtcgatg
    16621 gccgcctcgc gtcgggccat gagccgctcg tgccagagcc ggcaggtgcg cgcgtagtgg
    16681 cggcggagcc cctcgacgtc gtggacctcg agcccggcgg tctccatcac ctcgagcgtg
    16741 tggccgatat ggtcgagctc ggcgccgggg aagatgtagc gaaggatgat gcggcggctg
    16801 gccgaagggc ggcggaaggc cttctgctcc ttcttggcgc ggcgggtgat gccgtggttg
    16861 agcaggatgc cgccgggggc caagagcttg cccagcttcc tgaaataggc cgggtagttg
    16921 tcgatcccga catgctcgta catgccgatg ctggcgatct tgtcccactg gccgtcgagg
    16981 ttgcggtagt cctcgagggc cagggtggcg cggtcgccga ggcccaggct ctgcaccttg
    17041 gcgcgggcga ggtcgagctg cgcctgggac agggtgacgc catgcgcgat gacgccgtaa
    17101 tggcgggcgg cgtggcagac cagggcaccc cagccgcagc cgatgtcgag gaaccgctcg
    17161 ccggggcgaa gccgaagttt cttgcagatg aggtcgagct tggcggtctg cgcggtatcg
    17221 agatcggtct cgaaagtttc gaaataggcg caggaataga ccatctcggc gtcgaggaag
    17281 aggcggtaga agtcgttgct ggcatcgtag tggaactgga cgaggcgctt gtcgtcgcgg
    17341 gaccggctgt cgcgcgcctc ggcgtcaccg gcataggcat gggtgacgcg gtttgtcgcc
    17401 gtcggggcga aggcgagctt gcgggcggcg ttccagggaa agccggcgag cacgtggcga
    17461 aggcggcccc gatgctgctt ggccgcgccc tgcagccggt tcatcagcgc cagcaggtcg
    17521 tggtcgaccg gctcgatggc gccggtgacg tagaggcgaa agagcgtgtc gagggtcggg
    17581 cggcgtagca gggagcgcag ggtgcccggg ccggcgaccc tgatctcgtc gacgtccgag
    17641 cttgcacccg gggccgcgac ccgtcgcccg tcccacaggc gaaccgcgat cggcaagtcg
    17701 agtcgctcga ggatgtgggt gacgaggccg gtcgccgctt cgagcaggga ggcttcctcg
    17761 ggcggtgtcg tcatggcgtg ctccggcaat ggctcatcgg tcggcggcga agcggcgaaa
    17821 ggcgtcgagg gtctcgtcgc tgacatggtg ctcgatgccc tcggcatcgg cctgggcgat
    17881 gtcggcgctg acaccgagcg ccaggaggaa ggattcgacg atctggtggc gccggcgcga
    17941 ttcctcggcg agctcccggc cggtctcggt gaggaagacc gcgcgatagg gccgctgctg
    18001 gatcagcccc tcctcggcca gacgcttcag catcttggcg acggtcggct gcgccacgcc
    18061 gagccgggct gcgatatcca cctgccgcgc ctcgccgcca tcgtcgatga gatcggcgat
    18121 cagctcgaca tagtcctcga ccagctcgtt gcgccgggcc gcccggacgt ggcggaacgc
    18181 ctcgacatgc gtctccatgg cgaccagccc ctggacggcc ggctcgccgt catcggccgc
    18241 ctctggcgtt gctggcttgg cgacactgct gctcgtcatc tgcctgccat ccaatgaggg
    18301 gcgtgctcgt caatcgccgt ggggcttcat agcaatgtct ataacatgtg gcaccgcttc
    18361 gaatcgctcc agcgtcgtcg tcgtgaagag aggtgctgct caaccaacga gccggttgac
    18421 ccaaagttag cctttgctat atcagttagg ccatacgtga acaacaggcc catccggcag
    18481 catttgtcgt cgaggagacg cggacatgac cattggccgc gggcggcgga tcatcctctc
    18541 gtcggttgcg gccggcattc ttcttggcgc cggtgccgca gggacaggag tcgccgcgga
    18601 gaagctgaag gcggtgacca ccttcacggt cctcgccgac atggcgcaga acgtcgccgg
    18661 cgacgccgcc gaggtcgtct cggtcaccaa gccgggggcg gagatccacg gctacgagcc
    18721 gacgccgcag gacatcgtcc gggcgcacga tgccgacctg attctctgga acggcctcgg
    18781 tctcgagcgc tggttcgagc agttcctggc caatctcggc gatgtgccgg ccgtcgtcct
    18841 gagcgacggc atcgagccgg tcccgatcgg cgagggcgcc tatgagggca gggccaaccc
    18901 gcatgcctgg atggggctcg agaacgccct cgtctatgtc gacaatatcc gcgacgcctt
    18961 cgtggcgcac gatcccgaca acgccgcgac ctacgaggcc aatgcggcgg cctacaaggc
    19021 gtcgatccgc gaaaccatcg agcccttgcg cgagcgcatc gcggccatcc cggaggacca
    19081 gcgctggctc gtcacctgcg agggggcctt cagctatctc gcccgcgatt tcggcctgca
    19141 cgagctctat ctctggccga tcaacgccga ccaggtcggc acgccgcagc aggtgcgcaa
    19201 ggtcatcgat gccgtgcggg accaccggat cccggtcgtc ttctgcgaga gcacggtgag
    19261 cacggcacct gccgagcagg tggcacggga gaccggagcc gcctatggcg gtgtcctcta
    19321 tgtcgattcg ctctccgagg cgggcgggcc ggtgcccaca tatctggacc tgctccgggt
    19381 caccgcggag accgtggcca aagggctgga gggtggcggc ggatgagcga agcggtggcg
    19441 caggcgacgg ccgtcgagga gccggcaccg gtcgggtcgg agcccggcag cgggatcctc
    19501 ggccgcgacg tcaccgtgac ctaccgcaac ggccacacgg cgctgcgcca cgccagcttc
    19561 gccgttccct gcagcaccat cacggcgctg gtcggcgtca acggcgccgg caagtcgacc
    19621 ctcttcaagg cgatcatggg cttcgtgccg atcgccgcgg gcgagatccg catcctcggc
    19681 ctttcggtgc gcgaggcgct ggccaggaac ctcgtggcgt acgtgccgca ggccgaggag
    19741 gtcgactggg cctttccggt gctggtcgag gacgtggtga tgatgggccg ctacggccat
    19801 atgggcttct tccggcgggc ccggccggcg gaccgggcgg cggtcaccga ggcgctggcc
    19861 cgggtcggca tgagcgactt tcgcgagcgg cagatcggcg agctctccgg cggccagcgc
    19921 aagcgcgtgt tcctcgccag ggcgctcgcc caggacggcc gggtgatcct cctcgacgag
    19981 cccttcagcg gcgtcgacgt caagaccgag gagcagatcg tggccctgct caagagcctg
    20041 cgcgacgagg gccgggtgat gctggtcgcc acccacaatc tcggctcggt gcccgagttc
    20101 tgcgaccgca ccgtgctggt caaggggacc gttctcgccg atggcccgac cgcgctcgtc
    20161 ttcacccgcg agaacctgga gcgtgccttc ggcggcgtgc tccgccattt caccctcggc
    20221 ggcgagcacc tgcacgacga cgacgaccca cggcggatca gcgtcatcac cgacgacgag
    20281 cggccgctgg tgcgctatgg cggccggacc tggcggtccg ggacgaacgg caaggaccgc
    20341 aagctgtgat cgagctgctg ctgcagccct tcgactacgg ctacatgacc aacgcgatct
    20401 gggtctcggc gctggtcggc ggggtctgcg ccttcctctc ggcctatctg atgctcaagg
    20461 gctggtcgct catcggcgac gcgctcagcc actccatcgt gccgggcgtc gccggcgcct
    20521 acatgctggg cctgcccttc gcgctcggcg ccttcgtcgc cggcggcctc gccgcggggg
    20581 ccatgctctt cctcaaccag cgctcgggcc tcaaggtcga cgtcatcatc ggcctgatct
    20641 tcacctcctt cttcggcctc ggcctcttca tggtctcgat caacccgatg gcggtgagca
    20701 tccagaccat caccatgggc aacattctcg ccatcgacgc cgccgacacg ctgcagctcg
    20761 ccatcatcgg cttcgtctcg ctcgccgtcc tgctcgccaa gtggaaggac ctgatggtca
    20821 ccttcttcga cgagagccac gcgcgggcga tcggcctgaa gccagacctg ctgaaagccg
    20881 tcttcttcgt cctgctcgcg gcctgcaccg tcgccgccat gcagaccgtg ggggcgtttc
    20941 tcgtcatcgc catggtggtc acgccggggg ccacggccta cctgctcgcc gaccgcttcg
    21001 agcggctgct cgttgtcgcc gtcacgatcg gcgtctccac cggtgcggtc ggcgcctatg
    21061 ccagctactt cctcgacggt gccacgggcg ggatcatcgt cgccctgcag acgctgatct
    21121 tcctcgcggc cttcgccctc gccccgaagc acgggctcgt cgccgcccgg cgccgggcgg
    21181 ccgcagcgct cgcctgcgat gcgggtgcgt cgccggaagg cctgccttga gcgtcgccga
    21241 cctcctcctg cccttccagt tcccgttcat gcagaacgcg ttctggatcg tcctcgtcat
    21301 ctcggtcccg acggcgctgc tctcgtgctt tctcgtgctc aagggctggg ccctgatggg
    21361 cgatgcggtg agccacgccg tcctgccggg cgtcgtgctc gcctatgtgg ccggcctgcc
    21421 gctcatcgtc ggcgcctttg ccgccggcat gacctgcgcc ctggtgacgg gctacctcgc
    21481 gcacaacagc cgggtgaagc aggacaccgt catgggggtc gtcttctccg gcatgttcgc
    21541 catcggcatc atcctcttca cctcgatcga atccgaccag catctcgacc acatcctctt
    21601 cggcaacatg ctgggggtgg ggcgggcgga tctcctgacc tcgggcgcca tctcggccgg
    21661 cgtcaccctc gtgctgctcg ccaagtggaa ggacttcctc ctgcacgcct tcgacccggc
    21721 gcaggcccgg gcctcggggc tcaaggtggc gtggctgcat tacggcctgc tcgcgatcct
    21781 ctcgctcacc atcgtcgcca ccctctcctc ggtcggcatc atcctcgccg tgggcctcct
    21841 gatcgccccc ggtgcgatcg ccttcctcct cgcccgccgg ttcgggacga tgctggtgac
    21901 cgccatcgcc gtcaccatcc tcgccacctt cctcggcacc tatctcagct tcttcctcga
    21961 cagcgccccg gcgccgacca tcgtgctcgt gctcacgggc tttttcctgg tcgccttcgt
    22021 ccggcgcctg cgcctgaccc ggatggcctc ggcgagcgtc ggcgcgggcg agggctgacg
    22081 cacgattggc ttccccgacc ttatccgctt caggtgtcga taataggaat attatccgaa
    22141 gccgatgcgg ctgtcaagcc ggtgcccggt tcgttcggca ttatgggctt ttgcgcgccc
    22201 ggcgcgcgct ccggacgtgc cggaagctcc ggaaaaacgt tgtccggcaa gagcttgcct
    22261 gccgccgggt tcgttggtcg gcgcccgggt tcgttcgtcg aaaacgcggg ttcgttcgtc
    22321 cgaaacccgg gttcgatcgt cgatttcgcg ggttcgttcg tcgccgcctg tcgcggaatc
    22381 agcgacatgt ccctgcggat gtcgcagaat ccgcgacata aggaatcgtg atgtcgcgga
    22441 ttccgcgaca ttcggccggg tgctgtcgcg aaatatgcga caacccgggc cggcctgacg
    22501 cggattccgc gacaacccgg ggcgtgcagg gggtcgaagg gcggcaagtg gcgcgctcgg
    22561 cacgatttcc gggggacggc cataagcgtt cgtgatggcc gggcggtcga ggtggacaat
    22621 gtcacgcctg cggccgagct cgactgatgg ttcgatccgc aggccgcgag gatcgtgctg
    22681 cgggcggact gtcgaccgat cacatgcatt tccgcgctga gcgagcggca ccacgcgcct
    22741 caccgccgcg gcgggcgcca gccggcggcc agcgcctcgc cttcgctgca gaaccagcgc
    22801 tcgccctggc tctcggtgat cctggtgcgg tcgtagtgcg gtgaccaggg cgtgtggtag
    22861 atgcggtcgc cgctgctctg gctgatgttg cccttgatgg cgcagccgtt cggggccgac
    22921 tgctcggccg cctgccagcg gcgggcgcgg tagtcccagg gtggctcgag gcgcgcctgc
    22981 cagaggccgg ccagcttggc ttccgcctcc tcctgcaggg gtaccaggcg ggccgagtac
    23041 ctaacgaagg cgagggcgta gccttcggcg accagccagg ccgagaggtc ctggccttcg
    23101 tggctgcagg tggcgatgga cctgccgtag tcgtcgaggc cggcgaccgc gcaggtcacg
    23161 gtctcgcccg cgatgcggtc ggcgagaaaa ttggtggcca cgacgccgca atgctgcagc
    23221 tccctcgggc cgtcgcagcg ctggccgagc tcgggcgcgt cgatgtcggc gagccgcacc
    23281 accgtggtgc cgaggcggat ggtgtcgccg tcgacgacct cggcccggcc ggtgagcgtg
    23341 gtcgcggcga gcccggcccc gggcagcaag gccagcaggg cgagcagcga cagtcgatgc
    23401 cgccggggcg gcgccatttc gttgtttcct cccgcagcca cgacttcgcg gcgtctggtc
    23461 gatcaccggg agcataggcg cctggtgacg cggcggaagg gcgttcgccg cacgaagcgg
    23521 cgcaggccat ccccgggggc gccgagccgg tgaggctcag ggccgcacca gcccgtccgg
    23581 gatgtgggcc ctggcgtagc ggccgaaggc ggtgagggtg gcgggctctg gcctgggcca
    23641 gccggtggcg agggcggagg ggtcggacca gatggcgtgg gcccaggggc aatggccgac
    23701 gaagctgccg tcggggaagg cgtgggtctc gagccagcgg gtgaagccgg cgtcgggggg
    23761 ctcggccagg tcggtcggca ggtagcccca gccgccgcag aggaagggga tctcctgatc
    23821 gaggccgccg acgaggcggt cgaaatcggc ccattcgccg tccgcgaaca tgtggaagga
    23881 ggccaggaaa ttaccggccg gatcgtcgat cgggcgctcg cgccagcggc tgaagtcctt
    23941 ggcccagtgg ccgcccgaga ggctgaggag gttggtggcg ccggtggcgc ggatggtctc
    24001 gatcacgtcc tggtagaggg gctggacgtg gtaccactgg tcgagccggt cgaagcgggt
    24061 ttcgggcggg ttgtagtggg cggtcggctc gtcgaagagg ctgaaggcga tggcgccccg
    24121 gtcgccgagg cgggtcgcgg cgtcctgcca gaagcgggtc cagagcggga tcgacgagtc
    24181 gttgtagttg gtgaagcgga accagtggtc gatgtcctgg gacgccccgc cgggccagcc
    24241 gatgcaccac cagttgagga tcacgaagag gcgctggcgc tcgcaggcgt cgaggatcgg
    24301 caggaggtgg gcccagaagg cctcgggcga gcgctcgcga tactcggcgg gcgccaccag
    24361 gaggcggacg caattggccc cccagagccc ggcgatggtg gcgatctcgg cgacatgcgg
    24421 ggcggggccc agatcgccgg tgaaggcgtt gaagttgggg ccgtagagat ggacgggccg
    24481 gccctgccag cggagcccgg cgccggcgcg ccgcaggccg ggtgccgccg cacgcgccgg
    24541 ccgaccggtc gcgagaaggc cggtcgcggc agcactggcg agcaaccggc ggcgggtcgg
    24601 gccggcgagg gctggggggt cagccgacag cggtggccgg ctggcgctgc ggtgcccgct
    24661 ggccatattt gcgccagtag tagccgacgg tctcgtcgag cacgtggtcg agcgggcggt
    24721 gcggctgcca ggcgagccgc tcctgctcgg cgctgatgtc gacaacccgc cggtcgcaat
    24781 cctcgtagcc gtcgccgtag aactccttcg agccgatctc ctggatgggg tgctcgagat
    24841 agcggtcgtc gccgctgacc cgggcgaagg cgcggcgcac gccatgggcg aggtcgcgca
    24901 tgctgacctc gttggccggg ttgccgatgt tgtagaagtg gtcgagcgag tgctcggggc
    24961 ggtcgatgat cgcgaccagg gcgtcgatgg catcgtggat cgaggtgatg gtgcgcatgg
    25021 catggccgcc gtcgaccagc aggatcgggt cgtcgttgag cacggcgccg acgaacatgg
    25081 cgagcacgcg cggcttgcct tcgccctcga tgcccgggag gtagtccatg cgcggcccga
    25141 agaagttgaa cggccggacg atggcgaacc gcagcccggc ctccttgtgg taggcgtaga
    25201 ccagccgctc gagcagctgc ttcgccgtcg cgtaggtcca gcgctggttc tggaccggcc
    25261 ccatgatcat cggcgtcgtc gccgcatcca gcacatagag ctcggggttg tcgtaaggct
    25321 ggcccggcag gtagctcgac agcgtgcgac catagacctc gctggtcgaa tagtggatca
    25381 aaggcttctt gtacttggcg gcgagctcga cgatctgata gccatcgatg aagttggcat
    25441 agattgtgcg tagtggctcg gtgttgtatt gtgagggatt gcagattgcc gcgagattga
    25501 ccacccagtc gcaggcagcg aggtccttct cgaactggac gaaggcttcc ggccccttga
    25561 ggagccggcg ccgcagagtc agcctcgggt ggtcgagcag atgctcgatc ttgtccgtct
    25621 gcccgtccca gccgacaatc tcgaccgcct cgcgcgcgag catccgctcg agaaaatgac
    25681 tgccgataaa tccagcgcag ccaggcacaa gcaatttcat ggttcgatcc ccgcagtaga
    25741 cacccatcgc tgacatagag gggcagccgg ggcgaagcaa aaggtttgcc cgacgaccgg
    25801 gtcccccgtg atcgagtgtg tcattgttgc gtcgcgtcat ctacctgggg gcgaattatt
    25861 gtgcaatggc ttccgcgatc agcgaaaccg gggcgattct gtcgccgccg cgccacaacc
    25921 cgtcggcgac gaggccgcgg acgggtcgtc ggtgtggccg acgctggctc gtgctgtact
    25981 gtccgcatcg ggagaaacgc gggaattcgc aagaccagtg ggccatgtcg agccacgtcg
    26041 gggttggcgg gtcggcttcc tcgacaacag catgtcaggt gcagtcaaag ttgatcgaca
    26101 tggataaaaa tgtccaaaaa gttgaaagcg ggggagtcga tcaggacgtt cgatggcgcg
    26161 gcggcgaggc ggccccgaca aacaggcaac cggtgattgt aagcgaggac taccagtact
    26221 tcaaggagat cttcgcgcat ctgaaggagc tcgccggccg gcccgggctg cgtctcgccc
    26281 tggccgtcga cacgcgcgat gccgtgggcg aggtcgatgc cgacaccgtg gcgatcgtca
    26341 cctccgacga gaactgccag ctgccggtca atgtccggga tgccggcctc atcctgaagt
    26401 gccacggcgt cgagccctat ccgatgccag cttcgccggt cttgccggca gcgctggtgg
    26461 agcggctcag ggtggcgcgc gagcggcggc gctggcgggc ggacgcggcg cggctcgggc
    26521 cggccggggt ggcgggcctg ctggccaaga ccgagctcgt gccgatgggc tactgcaagc
    26581 agctcgacct gccgctggtg ccgttcgccg agcggcgccg ggtcttctcg ttccgcggct
    26641 cggtcgacaa ctaccgggcc ggcctgctct cccggcacgc ggtgaccaac taccccaagc
    26701 gcgtcgcccg ccagcgcttt ctcgcggcct tgcgcgaggt cgccagggag gcgccggctg
    26761 acgcctgcga cctgcgcatc accacggatt tcgacgacag cctgaaggat gccgggcgcg
    26821 actactcgac agcgctgatg gacagccgct tctgcctctg cccgcgcggc acgcggctcg
    26881 agaccttccg catcttcgag gggctgcgct acggctgcat cacggtgacc gaggccttgc
    26941 ccgaccgctg gtacctgcgg ggagcaccac tgctttcggt cggtgactgg cgcgagctgc
    27001 cgggcctgat gcggcggctc ctggccgatc cggagcgcat ggcggagctg caccatcgct
    27061 cgctcgcctg gtggcgcgac atgatttcgc ccctggccat cgcgcgccgg gtgctgccgc
    27121 ggctcgaacg gggtggcccg gcccgccacg ccgccgtcgg gtcggcggga gggctcggct
    27181 gatggttctg gcccttttcg tgctggctgc cctggcgctc tttctcggcc tctactcctt
    27241 cgtcttctac gccccgagcc tcgccctcct gccggcccgc ccggtggccc ggcgcgatgt
    27301 ggcggacggt cggcggccac gcatcaccct gtgcttctgc gcctatcgcg aggagcaggt
    27361 ggtgccgctg aagctccaga acctgcggct gctgcgcgag cgccacccgg atctggagat
    27421 cctggcctat gtcgacgcct cgccggatcg cacggccgag ctgctcgagg cggcgccgga
    27481 gctcctgacg gtgatcgtcg gcgaggagcg gcgcggcaag agccacggca tgaaccggct
    27541 ggtcgccgcc gcgaccggcg acatcgtcgt cttcaccgat gccaacgtgc tgctcaagga
    27601 cgatgcgctc gacgtgatcg ccgcgcattt cgccgacccc gaggtcggct gcgtctgcgg
    27661 ccggctgctc tatgtcgacg accacaaccc ggccgcacac gtgaactcgc tgatgtggcg
    27721 gctcgaggag cggatcaagc gggccgagag ccggacgggc tcggtgatcg gcgccgacgg
    27781 ctcgctcttc gccatccgtc gggccctgca ccggccgccc ccggcggaca tcatcgacga
    27841 tttctacgtc tccctgtcga tcctgctcga cggccaccgg gtgatctccg caccggatgc
    27901 catcgcctac gagaactcgg tgcacgaccc ctatgaggag atgcagcgca aggttcgcat
    27961 cgcctgccag gcctggaacg tccaccgcct gctctggccg cggctccgcc ggctggatgc
    28021 ggtgcatctc tacaaatacg tcgcgcacaa gctgctccgc tggttctcgc ccttcgccct
    28081 ggcgctggcc gtgctgctcg tctcgacggc cctgctcctc gccttgcccc tgaccctgtt
    28141 cctgcagctc ttggtcgccg ggccgctcgc cctggcgctc gggctctggc tccgcctggg
    28201 cgccgccgag gcgacgcaca gcacggtcct ctcgttgctc ggcgtcggca tcggcatcac
    28261 ccgctcgctg cggggcgagc ggttccagac ctggaccccg gcgacctcgg cgcggcagcg
    28321 cctggagcgg ccgcggctcg tctatttcag ccacgaccgc aacgacgcca atgtctggca
    28381 gcgctgccag gatttcaccg ccctcggctt cgtgctcacc aatgtgagct ggcatcgcga
    28441 caaaaactcg caggacggcg agcggccctg gcacgagatc gatctcggcc ggacggtcga
    28501 tgccgcctac tggcaccgcg cctggagcgt ggtgcggggg gccttcggcg tgctctgcca
    28561 cttccagcag ctccgggacg cggatctcgt cgtcgcgcgc aatttcgagc cgtccgtcct
    28621 cgccatgctg gcccgccgcc tgttcaatcg ccgcgtgcct ttcgcctatg agtgtgtcga
    28681 catccaccgt ttcctcacca gccgctcggt gctcggcgat gcctgccgcc tggcggagcg
    28741 ccgggtgctg gcggccgccg acctcctgat cgtcagctcg cccgatttcg ccagccgcta
    28801 cttcgcgccc gtgcacggct atgacggccc ggtctatctg ctcgagaaca agatgccgag
    28861 cttcctcgcc cggcgcttcg ccgccccgac catgctgcgt cggccgcatg tcggcccgcc
    28921 ctggcggatc ggctggttcg gcatgctgcg cgacatggag agcctgggcc tgctcctgcg
    28981 gctcgcggcg atgcgaccgg agttggtcgn nnnnnnnnnc tacgggctga ccgagacctc
    29041 cagcacgatc gccctcctcg gctccgacga ccaccgcgag gcgttcgcca gcgacgaccc
    29101 cgcggtgcgg ggtcgactcg cgtcggtcgg ccggccgctc cccagcgtcg aggtctcgat
    29161 ccgcggctac ccgaccgaga tcaccgagaa cgagctccgg cagatggtcg agccctatcc
    29221 caacgtcacc tatggcggcc cctaccagaa ggcgacggag ctccgctcga tctacgagaa
    29281 cgtcgacctc gtctgggcca tcgatctcta cgatgccggg gtcaactcgg actggctcct
    29341 gcccaaccgt ctctacgagg cggggttctt cggccggccg gtgatcgccc gggccgagac
    29401 ggcgatcggc cgcttcgtcg ccgacctgca gatgggctgc gtgctctcgc cgccctatga
    29461 cgtcgcctgc ctggatctgc tcggccagct ctctggcgac cactacgccg cgatcgtccg
    29521 ctggatggag gagctgcccc gccagcgctt cgtgctcgac gaggagctgc gcgagatgac
    29581 gcagaacctg ctctcgccac aggcggcggg tgcagtcgtg gccgagctgc ggaacagcaa
    29641 cgttgcctga tcggcccgag gcggccgtgc cgaaagcccg ggccgcccgg ctcgagcata
    29701 tcgacagcct gcgcggcgtc gcctgcctcc tcgtcgtcta catccacgcg gtgcagtggc
    29761 tcgagcgcaa tgcctttccg atgtcgggcc ccgaggcgac gctgctgcac gcttcgatca
    29821 cctggctcga tcccggcaag gtcggcgtgc tcgtcttctt cgccatcagc ggctatgtcg
    29881 tgcccttctc gctgcgccgc ccgctcgccc atcccttgcg cgagttcgtg gtcaaccgct
    29941 tcttccggct gatgccggcc tactggttct cgctggtcgc cggcgtcctg atcatcatct
    30001 ggttcgagca gcgcccgacc gatctcgccg gctggctcgg caacctgatc ctcgcaccgc
    30061 agctcctcg
//
LOCUS       DAOTFI010000018        29213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1805_length_29213_cov_6.914809, whole genome shotgun sequence.
ACCESSION   DAOTFI010000018 DAOTFI010000000
VERSION     DAOTFI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 29213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29213
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1805_length_29213_cov_6.914809"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="P5558_02960"
     CDS             complement(<1..284)
                     /locus_tag="P5558_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY23303.1"
                     /translation="MTALTPREIVSELDRFIVGQNAAKRAVAIALRNRWRRQQLDPAL
                     RDEVLPKNILMIGPTGCGKTEIARRLARLAQAPFVKVEATKFTEVGYVGR"
     gene            complement(281..838)
                     /gene="hslV"
                     /locus_tag="P5558_02965"
     CDS             complement(281..838)
                     /gene="hslV"
                     /locus_tag="P5558_02965"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRY23304.1"
                     /translation="MTGTDASLWHGTTILAVRKGGQVVIAGDGQVSLGNTVIKANARK
                     VRRLGGGAIIAGFAGSTADAFTLLERLEGKLERHPGQLMRACVELAKDWRTDRYLRRL
                     EAMMAVADAEVSLVLTGNGDVLEPADGLIGIGSGGNFALAAAKALIDIDALDARAVAE
                     KAMGIAAEICVFTNRQLTIETIGQP"
     gene            complement(988..1248)
                     /locus_tag="P5558_02970"
     CDS             complement(988..1248)
                     /locus_tag="P5558_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23305.1"
                     /translation="MTHQAAVNDNQPALPELELADLELPDEVAAALDDPRLENVVGAA
                     MLVATAFRLRDEEALIMMLRRLTNAVEGLSRDEAPARRAACG"
     gene            1806..2552
                     /locus_tag="P5558_02975"
     CDS             1806..2552
                     /locus_tag="P5558_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY23306.1"
                     /translation="MAGHSQFKNIMHRKGRQDAVRAKLFTRLTREVQVASRMGLPDPA
                     FNPRLRAAIQAAKAANVPKDNLERAIKKGQGGDTESYDEVRYEGYGPGGVAFIVEALT
                     DNRNRTASEVRAAFSRHGGSLAETNAVSFQFDRIGQIRFAAEVADEEAMLEAAIEAGA
                     EDCQSSEDGHEITCAPDDLAQVTEALEARFGTPEASLLTWRPQVTVPVEDDKAPTLFK
                     LLEVLDESDDVQRVVANFEVSDEAMANLQA"
     gene            2552..3061
                     /gene="ruvC"
                     /locus_tag="P5558_02980"
     CDS             2552..3061
                     /gene="ruvC"
                     /locus_tag="P5558_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRY23307.1"
                     /translation="MRLLGLDPGLQRTGWGVITVNGPALGFVAAGVVASVSSETLAER
                     LLALDRGIALVIEQHVPDEVAVEETVVNKNALSSLKLGHARGVALLTAARAGLPVAEY
                     AAKHVKRALTGTGGALKPQVAHMVQVLLPRAGPVTADAADALAVAICHAHNRMTLARI
                     HGAVMATVR"
     gene            3058..3681
                     /gene="ruvA"
                     /locus_tag="P5558_02985"
     CDS             3058..3681
                     /gene="ruvA"
                     /locus_tag="P5558_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRY23308.1"
                     /translation="MIALLRGRIAAQGEDHVVIDTGGEAGGVGYLVQCAGKTLHRLPP
                     TGQRAELRIITQVREDSITLYGFLTPVEEQWFRILQGIQGVGARLALAVLSVLEPEEI
                     GRAIAAQDARALTRAAGVGPKLAGRIVAELKDRVAKLPAALAIGGAARPAPAPAGGVV
                     EDALAALQNLGYGRSEAYAVVARVQNELGIDATLEQLLAASLKGLAR"
     gene            3678..4757
                     /gene="ruvB"
                     /locus_tag="P5558_02990"
     CDS             3678..4757
                     /gene="ruvB"
                     /locus_tag="P5558_02990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRY23309.1"
                     /translation="MSGPQGEEGRLVGGALQAADADVSLRPKRLAEFIGQAQLKSNLA
                     VFVEAARSRGEAMDHVLFAGPPGLGKTTLAQIVAAELGVGFRMTSGPIIQRAGDLAAL
                     LTNLQPRDVLFIDEIHRLSPTVEEVLYPAMEDFQLDLMIGEGPAARSVRIDLAPFTLV
                     GATTRTGLLTTPLRDRFGILARMEFYGQAELLEIVERGARLLRLGLTGEGAAEIARRS
                     RGTPRVATRLLRRVRDFATVEQPNGASPVDRIRADAALQRLAVDRLGLDALDRRYLNA
                     IAVHYGGGPVGVETLAAALSEQRDTLEETVEPYLLQQGLLQRTPRGRLLTQASWRHLG
                     LAMPASAVAQAELFAGPDDAGEGEP"
     gene            4754..5179
                     /gene="ybgC"
                     /locus_tag="P5558_02995"
     CDS             4754..5179
                     /gene="ybgC"
                     /locus_tag="P5558_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HRY23310.1"
                     /translation="MSAAHRLPVRVYYEDTDAGGIVYHARYLCFAERARTEMLRSLGV
                     EQRVLRDEADGVFAVRRAVAEFLRPARLDDALVVETELRHVRGARLGLLQNITRGGQV
                     LVSIKVEIVFINSDLRPRRLPPWLALRFAEAGHSVAGEA"
     gene            5331..6056
                     /gene="tolQ"
                     /locus_tag="P5558_03000"
     CDS             5331..6056
                     /gene="tolQ"
                     /locus_tag="P5558_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HRY23311.1"
                     /translation="MESDPISSAMLGEPTAGMSILALALEADIVVKFVMLLLLVASLW
                     VWAVVFEKGFRLVRLRRQTALFEQAFWSGTPLGELYRRLGKRIDHPMALMFAAGMEEW
                     RGGPKAVSGDAQDRLVGRIGQVMQLTLEREMRGLERNVSTLATIGSAAPFVGLFGTVW
                     GIMNSFQSIAMTKNTTLAVVAPGIAEALFATALGLLAAIPAVIAYNKISTSVDGLGDR
                     LASFADEFGVVVSRELDSSRVVP"
     gene            6056..6517
                     /gene="tolR"
                     /locus_tag="P5558_03005"
     CDS             6056..6517
                     /gene="tolR"
                     /locus_tag="P5558_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HRY23312.1"
                     /translation="MAATAVGGTGGRGGKWRRGRQRRQMGEINVTPFVDVMLVLLIVF
                     MVTAPMLTMGVSVDLPRADSSPMPGQDEPLSVSIDASGTIFLQETAIELDQLAPRLLA
                     ITEQNAEARIFVRGDRAIDYGRVMAVIGNLNRAGFTKVALLTEQPDLVVAE"
     gene            6522..7574
                     /locus_tag="P5558_03010"
     CDS             6522..7574
                     /locus_tag="P5558_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23313.1"
                     /translation="MNRSFIASVLFHLVFIGLAVLGLPRMAERLPIDEPFTVEIVGIP
                     AAPEPTEEPPAPPPAEAAPLPPRQAASPAPPPPAPEPEPEPEPAPEPPQQLAAPEPPP
                     PPPAPEPPPPPAPEPPPPPPPPPAAEPVPAQAAAPVPPARPRARPEPPKPVETAAPAP
                     EPPAPEPPKVEPPAVKVAARPKPPEKPAPPKKAEPSPAPVAKDDNDFNALLRSVEQQN
                     RRVEAPERRPGTGNAEAARTAPPAAMAAPVALSASELAGIRRQIERCWNVPIGVPGIE
                     AMRVSLRIQLEADGSVREVSIEESGRFDADHGFRTVAESARRAVLSCRLDLPAEKYAV
                     WRDMVLTFSPGDALSG"
     gene            7567..8919
                     /gene="tolB"
                     /locus_tag="P5558_03015"
     CDS             7567..8919
                     /gene="tolB"
                     /locus_tag="P5558_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRY23314.1"
                     /translation="MADHLKDRRPGGPTRRAMLAGATAGLGLLAMAGGAAAQVRLDIT
                     KGVVEPIPIAVSPFAGEDVGTRDIGRAVADVIAANLESSGLFRVIDRRAYIQDPESLR
                     ETPRFAEWRPINAQALVTGVVSRPAADRLVIEFRLWDVFSGSQLRGLRYELAADGWRR
                     VAHQISDVTYERLTGESGYFDTRIVYVSESGPRTQRVKRLAVMDQDGHNHRFLSDGGQ
                     LVLTPQIAPDGRRVAYFVLRTPVPQIMMLDLATGRSAPLGEFPGMSFTPRFAPDGSVL
                     LLTVARDGNSDIFSFDTATRRTTQLTQSPAIDTSPSFSPRGDRIVFNSDRGGTPQLYT
                     MNRDGSNVQRISFGTGRYGSPAWSPRGDLIAFTKIQGGYFHIGIMEPDGANERLLTRS
                     FLDESPAWAPNGRIIVFTREDPRTDRSRLMTIDITGYNERELPTPLDASDPAWSGLIP
                     "
     gene            9030..9605
                     /gene="pal"
                     /locus_tag="P5558_03020"
     CDS             9030..9605
                     /gene="pal"
                     /locus_tag="P5558_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRY23315.1"
                     /translation="MRPLFAILPAALLLLAACSSTPEDTLGSDEQAGGATSSVDGAYG
                     AGGAPTELGDGSSTVIVEEDLFASGAPGSQQDFEINVGDRVFFGFDSTTLDDDARATL
                     DRQASWLFQYPATSVTIEGHADERGTREYNLALGERRATAVKNYLVALGVTPDRLLTI
                     SYGEERPVDPGRDETAYAQNRRAVTVINLVN"
     gene            9689..10651
                     /gene="ybgF"
                     /locus_tag="P5558_03025"
     CDS             9689..10651
                     /gene="ybgF"
                     /locus_tag="P5558_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRY23316.1"
                     /translation="MSLRLAVSLAACLVGFTPAVAQDPQPLRIERAAPAPAPLQQPGA
                     AAPAGQAVDGRAMAVMQNRIAALEEEVRRLTGRLEEAEFQDQRTRQRIDDLVADLDRR
                     LMALDQAAMAQTAPEGRAAPPPADDAGSVGPALVAPGAAGGDAAAADVEPDPAAREGH
                     VLGTIPRDAVLGLPQPDGSGPSGTAPRATLEGTAAARFTASLDLLQTGDWSAAEVAFD
                     RWLADFPDDPQAPVAAYWLGESQLAREDFATAAATFAGNYRTYGDQAPRAPDNLLKLG
                     TALAAMGDQSRACQTFIEMERRYPNASPALLQARERESRAAGCG"
     gene            10701..11990
                     /gene="tilS"
                     /locus_tag="P5558_03030"
     CDS             10701..11990
                     /gene="tilS"
                     /locus_tag="P5558_03030"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRY23317.1"
                     /translation="MAALGPFEPRPAIAAGVSGGPDSMALALLLRDWVASRDGRLLAL
                     VVDHGLRPGSADEAALVVERLAALGIAALVLRVTGDWPSGSIQATARARRYALLEAAA
                     ERAGMLHLVVAHHAADQRETVAMRAAAGSGPRGLAGMAPIRELCAVRLLRPLLPVEPA
                     RLRALLRARSVDWLEDPSNRDPRFWRARARAAGELPALVNDTTERAALEHRLARFLAG
                     HAQVHPAGFVELSLDALSALAADERRQLLGRLVAAVGGRAFAPRGRSLARLDAWLCDP
                     SSPRCSLGGTLVERRRTRLRVLREARGLPAELALAGGRTIWDGRFRIDWLGAEATAGG
                     PPSLRAAGQGGGGARALRAWLAAVGAPEPAAALPALVLATLPLVSRDAQVLALGPWVA
                     GAARGVLAVVFRPTHTMTAVGSAPSVCCADEQLTYLG"
     gene            12092..14026
                     /gene="ftsH"
                     /locus_tag="P5558_03035"
     CDS             12092..14026
                     /gene="ftsH"
                     /locus_tag="P5558_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRY23318.1"
                     /translation="MNNFSKNLALWIIIGLLLIALFNLFQGPSSRGPQTSMAFSDFLS
                     EVEAGRVNEVTIKGDSISGTFADGRQFSTYAPFDPNLVDRLTANDVRISAQPNDDNMP
                     SLLGVLVSWFPMLLLIGVWIFFMRQMQSGGGKAMGFGKSRARLLTEKHGRVTFSDVAG
                     IDEAKEELQEIVEFLKNPQKFQQVGGRIPKGCLLVGPPGTGKTLLARAIAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIVFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRFDRQVVVSNPDVLGREKI
                     LNVHVKKVRVGPDVDTRVIARGTPGFSGADLANIVNEAALLAARRGKRMVTMDDFESA
                     KDKVMMGAERRSMVMTEEEKKLTAFHESGHAVMAYYSPASDPIHKATIIPRGRALGMV
                     MRLPLGDRISVSREKLKADIAVAMGGRVAEEQVFGHEKVTAGASSDIQQATKIARYMV
                     TQWGMSDLLGPIGYDENQQEVFLGHSVTQSKNVSEATAQQIDGEIRRIVEEGYHLAQE
                     TLRDRADDLHAIANALLEHETLSGEEITAILKGEPMPAPRKAGDDAASSPPSGRRGSV
                     PTTRPADDGPSGDPAPQPGA"
     gene            complement(14057..15142)
                     /locus_tag="P5558_03040"
     CDS             complement(14057..15142)
                     /locus_tag="P5558_03040"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRY23319.1"
                     /translation="MRNPLTAAQWRGLGLALAIGFAGGLLFSWLGWPLPWMMGPMIAT
                     TLAALTRLPVAVPVTLRDPMMAIIGVLLGSGFTPELVANLWTWLPTLLVLPIYVALVA
                     VSSMTYLRLVTRFDPRTTFFAGMPGGFGEMVLLGDRAGGDARRIALVHTVRVLLIVLT
                     VPLLVRAFGIELPSALPAAARAGWLEVAGLLLAAGIGGWLGTMLRLPAPTLLGGMVVS
                     AIGHLTGLLEGAPPVLFVAIAQLVIGCSIGARFVGFRLHDVLATMIAGIGLTLVMFAA
                     SFVVAALVHLATGTEPLLLLLALVPGGVAEMSVIALALGVDPAFVATHHIVRIAIVVM
                     TAPFVFRLLERRYRPPGSKDAGPKAGV"
     gene            complement(15151..15564)
                     /locus_tag="P5558_03045"
     CDS             complement(15151..15564)
                     /locus_tag="P5558_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRY23320.1"
                     /translation="MAQWLMKTEPEDYSWDRLVAEGGTGWDGVRNHQAAANMRAMQKG
                     DRAFFYRSLVDPAVIGIMEITRAAYADPDDPAGKWVRVDVAPVRPLPREVSLKAIKAD
                     PALADLALVRQSRLSVMPVSDAHWALITRMAGLDA"
     gene            15806..16738
                     /locus_tag="P5558_03050"
     CDS             15806..16738
                     /locus_tag="P5558_03050"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRY23321.1"
                     /translation="MKLLAGNSNRPLAEAIAAYLRVDLTKATVHRFADMEVFVEIHEN
                     VRGEDVFIIQSTSYPANDNLMELLVCIDALRRASARRITAVIPYFGYARQDRKPGPRT
                     PISAKLVANLITEAGANRVLTLELHAGQIQGFFDIPVDNLYGAPLMGPDVQSNFGTDD
                     VVIVSPDVGGVARARAYAKRLDANLAIIDKRRERAGISEVMNVIGEVDGRHCVLVDDI
                     ADSGGTLCNAADALLAEGAVSVQAYVTHGVLSAGAVARIAASKLDRLVITDSIRPTEG
                     VRVTEKIRVLSVAQLMGEAISRIASESSVSSLFD"
     gene            16830..17465
                     /locus_tag="P5558_03055"
     CDS             16830..17465
                     /locus_tag="P5558_03055"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRY23322.1"
                     /translation="MSETIQLTAEPRERTGKEGARALRKTGMVPAIVYGGKEPPQKVA
                     LVDREIRRQLELNPRFFSSVVELKLGKKVIQVIPREAQLHPVKDHPLHVDFLRAAKGS
                     TVTVEVPVHFLNEERCPGLRRGGVLNIVRREIELVCPVEAIPERIDVDLAAVEIGDSV
                     HISAVTLPANVTPTITDRDFTICAVVGRGPTADEDEEGEGEGAEAGGEADD"
     gene            17492..18061
                     /gene="pth"
                     /locus_tag="P5558_03060"
                     /pseudo
     CDS             17492..18061
                     /gene="pth"
                     /locus_tag="P5558_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391494.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            complement(18557..19903)
                     /locus_tag="P5558_03065"
     CDS             complement(18557..19903)
                     /locus_tag="P5558_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRY23323.1"
                     /translation="MRRLFDDHLLRRIARNTGLLLSGNGVAGILALGSVTVAARGLGA
                     EAFGVLMIFHAYAMIMGGLCRFNVYQAILRYGASCLADQRRRDLQGLLTLGVGVELAG
                     VAAAMALGVVALGWLGPRLGLDEAYRETALWYCLVVIASNGATPIGILRLLDRFALIA
                     WTRPVTPTLRLVGCLAAWAGGAGLPVFAAVWALAGSAEALLLWRLAHLELHRGGWLKD
                     LRLRVRGLVRPHQGLWRMLLWTNLQGTLGMVSGRLATVLVGAMLGPVAAGLYLVAYQV
                     AAVIERPLQIVRRAVDPEFARLVAAADMATLWRLFRRTLLGTACFAAPLLVVLGLFGD
                     RLLGFFFGEAFAAAHPVLVLLALQTALLALVMPSASVLVMLGHAGRLLLAQAIGRGAQ
                     LLALLALMQHFGLVAAGAAGAAAAGLELVLVTGFVLATRRAAAPDAATAAGGCAGR"
     gene            20006..21244
                     /locus_tag="P5558_03070"
     CDS             20006..21244
                     /locus_tag="P5558_03070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY23324.1"
                     /translation="MPISDAAGKKRPQEAACPINGILGSMHIGMFFSGLAGGGTQRRM
                     LALARGFAARGHEVDILVASAEGAFRAEVPPDAGLVALGADWRRLPLIGRHRGLWVPL
                     AARALGRRLRAAPPDVLLASSTPANLTASLVRRRAAPAVPLVLTLNLPPSAVSGGLGR
                     GGRPLRALMRRCYAEADALVAISAGVAADAAAMLDLPAARIAVVMNPIDTARIQALAG
                     QDPGHPWLEPTAPPLLLAVGKLKAQKDFPTVLEAFARVRAVRPARLVVLGDGEERQSL
                     VHLAGRLGIAADVAFMGFVANPFAWMARARVFVLGSRFEGFSNVLAEALAVGCTIVST
                     DCPHGPRELLDEGRHGRLVPVGDASAMAEAMLAALDDPADPGRQRARAARFSVGRAVD
                     GYLEVLTAVAAPDRHLRRAG"
     gene            21244..22458
                     /locus_tag="P5558_03075"
     CDS             21244..22458
                     /locus_tag="P5558_03075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY23325.1"
                     /translation="MDRQERRLAILVRALDGGGMQRNTKRLAGAFAARGHPVDVLATD
                     VSGPIRQGIESVARLVALAGGSHVTGRLLALRAGWRERTALRPLLLGGGPGILAHLPA
                     LVAYLRLARPTALLALGTQCNLAALWARRLAGTHTRVVVSERNTLSLVVANTRRRFRR
                     AYPALVARTYPEADGIVAVSRAVADDLARTCHLAADTITTIPNPVPEPEDRGADDRAG
                     NAPAHPWLGPDQPPLILGVGRLNWQKDFATLLEAFALVRRRRPARLAILGEGEERQRL
                     EARARELGIAADVVMPGFVDDPFAWMARARLLALTSSQEGFGNVIVEALASGCPVVAS
                     DIPGGPREILDGGRYGSLAPPGDAGAFAEAMLAAMDDPPARALLVGRAGDYALGPIAE
                     RYLALLLDKELE"
     gene            22455..24089
                     /locus_tag="P5558_03080"
     CDS             22455..24089
                     /locus_tag="P5558_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6455 family protein"
                     /protein_id="HRY23326.1"
                     /translation="MTIDTDDGVFAEDAAALHALVPSTPAKHIAVFIYALTGGGAQRR
                     TVTLANGFAECGHDVDLVVVHARGVIHHELAPRVRLVDLGDGYGRLHAAACRRGAPRG
                     LQTALAVPALARYLRRRRPDVLLSAASHVNLVAVAAARLSRRPIPLVLRASNHPSGNL
                     AHHPPVQRLVRAWLKWLARRFYPRADAIIAVSSGVARELGRLTALPPERITVIDNPVL
                     TARLRRRMAEPLEHPWLAPGSPPVILAAGRFTLQKGFPTLLEAFRRIARDCDARLVIL
                     GDGPGRETLAARAKALGLGERVLMPGYVDNPLAWMCRARLFVLSSLWEGLPGVLVEAL
                     ACGLPVVSTACPSGPAEILEDGAHGTLVPVGDAEAMGRAILAALAARPEPERLRARAE
                     HFALAPSIKAYLDVLEAVIRRRAPERLAWSPPWPLTVRLDWTSPEGLSRALGRVCRAP
                     GDLFRAWPGNADHRQRMARMMQAFAIDPDRAADTRWQELVAADARCAGCVRIARCAAW
                     LARPGPAAEAASFCPNAGLFGTLADEAVRERAAASA"
     gene            complement(24144..24641)
                     /locus_tag="P5558_03085"
     CDS             complement(24144..24641)
                     /locus_tag="P5558_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23327.1"
                     /translation="MHSNQTIDVVAPQADFAFDAARTFVGPHASYYDETWRQMAWRGR
                     QTSWNRHAALYGPFWFAYRRLRLVALASVLWLLLWLELWRHGWSPWLLCGLQLAAMLV
                     QGTFANRLYLGRFTALGRRLERSLVDAAARERAMARTGGVSRRDVVAAMLALALGLAL
                     LELRR"
     gene            24970..25431
                     /locus_tag="P5558_03090"
     CDS             24970..25431
                     /locus_tag="P5558_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRY23328.1"
                     /translation="MSTVRFDPFFRTVRDLDRMTDELLGSRGGFAPEYDIEDQGEDRF
                     AIALALPGYDLDELEITAKDRQLVVKGEPKAGNRANYIRRGIARASFQRSFTLGEHIE
                     IAGASLDKGILRVELERRVPEAMKPRTIAIAAPAAGQPAPQPEAAAVEQAA"
     gene            25555..26463
                     /locus_tag="P5558_03095"
     CDS             25555..26463
                     /locus_tag="P5558_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRY23329.1"
                     /translation="MSDELYTTLGVGKTASDDEIRKAYRRLVKTCHPDTNPNDKRAEE
                     RFKALGRAYAILGDPDKRKRYDRGLIDADGNERGPQGFGGGFGGFDPGRAGARRTYHR
                     SAGHRSAGPNAEFGGFAGFDDVLSDLFGGRTGRRANARARGEDLKLTVSVDFLEAVKG
                     GKRRVGLPDGRHLDVTIPAGIENGRVLRLKGQGMAGGDALIEVQVRPHEGFRRDGLDI
                     HLDHSIPLDLAIAGGRVTVPTIDGPVAVTVPPWTSSGRTLRLKGRGIGQGGRQGDQLV
                     RLEILLPDRADETLQRWARDRVKATA"
     gene            complement(26657..27649)
                     /locus_tag="P5558_03100"
     CDS             complement(26657..27649)
                     /locus_tag="P5558_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRY23330.1"
                     /translation="MISLFPLPRFGGKRRFDQLSEQEILALAISAEEEDGRIYALYAQ
                     KLQDAYPASAAVFIGMAEEENRHRHRLIELYRKRFGELILPIRREHVADFYSRKPVWL
                     VENLGLERIREEAQAMERQAHAFYVAAAGRTADAETRKLLGDLAAAEAGHVRKAGDLE
                     ARHLDDDTRGEEDSAARRQFILTWVQPGLAGLMDGSVSTLAPIFATAFATQSTWTTFL
                     VGLSASIGAGISMGFTEAAHDDGKLSGRGSPIKRGLASGIMTTLGGLGHALPYLIPEF
                     WTATVIALIVVFIELWAIVWIQNRYMETPFARATFQVVLGGALVLAAGILIGGA"
     gene            complement(27725..28954)
                     /locus_tag="P5558_03105"
     CDS             complement(27725..28954)
                     /locus_tag="P5558_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY23331.1"
                     /translation="MVRLASFTALFVSAGLLLGGNGLFGTLIAVRANLEAFSPTMIGL
                     LGSVFYAGFVAGSLYAPRLIIRAGHIRTFAAAGAMYAVAALLHAMLAEPWLWLLLRTL
                     GGFCFAGLTLVLESWLNERTPNSDRGRVLSVYRLVDLACVTLGQLVLALADPRGFELF
                     SLVAILFCCCLVPLAMTRSTLPAPLQTPRLRLGRLIAVSPLGTIGVFSVGLVNSTFRT
                     VAPVALLDQGLTIAEIAWLMSAFIAGGAVMQFPIGALSDRLSRRTVLLATTTGAIATS
                     FAMSVATGLSPTAIILTGFLFGGFAIPIYSLSVAHANDLAQPDEFVEVAAGLFFVYGI
                     GAMVGPLLGAGAVALLGPQALFWYTSGVHGFFLLFTVYRISRRPRLPTTGREGFVGLI
                     RTSPGIFKLDPRGRERK"
     gene            complement(28959..>29213)
                     /locus_tag="P5558_03110"
     CDS             complement(28959..>29213)
                     /locus_tag="P5558_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY23332.1"
                     /translation="IFVGPAQSASRTLMARLSPAGQRNEMFGLYALSGKLTAFLGPFL
                     FGAATTLFATQRAGMAVVVLLLLAGGLLLLLTVREPAPQG"
BASE COUNT         4325 a        10335 c        10502 g         4051 t
ORIGIN      
        1 tcgcggccga catagccgac ctcggtgaac ttggtcgcct cgaccttgac gaagggcgcc
       61 tgcgcgagcc gcgccaggcg gcgggcgatc tcggtcttgc cgcagccggt cgggccgatc
      121 atcaggatgt tcttcggcag cacctcgtcg cgcagcgccg ggtcgagctg ctggcggcgc
      181 cagcgattgc ggagcgctat ggcgacggcg cgcttggcgg cgttctggcc gacgatgaag
      241 cggtcgagct cggagacgat ctcgcggggg gtgagggcgg tcatggctgc ccgatcgtct
      301 cgatggtgag ctggcgattg gtgaagacgc agatctcggc ggcgatgccc atggccttct
      361 cggccaccgc ccgggcatcg agcgcgtcga tgtcgatgag cgccttggcg gcggcgagcg
      421 cgaaattgcc gcccgagccg atgccgatca ggccgtcggc gggctcgagc acgtcgccat
      481 tgccggtgag caccagcgac acctcggcat cggcaacggc catcatggcc tcgaggcggc
      541 gcagatagcg gtccgtgcgc cagtccttgg cgagctcgac gcaggcccgc atgagctggc
      601 ccggatggcg ctccagcttg ccttcgagcc gctcgagcag ggtgaaggcg tcggccgtgc
      661 tgccggcgaa gccggcgatg atggcgccgc cgccgaggcg gcggaccttc ctcgcattgg
      721 ccttgatgac cgtgttgccg aggctcacct gcccgtcgcc ggcgatgacc acctggccgc
      781 ccttgcggac cgcgaggatg gtcgtgccat gccagaggct ggcatccgtt cctgtcatgt
      841 ttccgccctg cgcccttgcc tgtccggacg ccgcggcgcc cggtcgagag cggcatttgg
      901 gcagggcggg ggggatcgtc aaggagacgg ccggcggcgg gtgccgtcgg ccgtggcggt
      961 gggggggagg agaggcggcg ggaaccctca gccgcaggcg gcgcggcggg ccggcgcctc
     1021 gtcgcgggaa agaccctcga ccgcgttggt caggcggcgc agcatcatga tgagcgcctc
     1081 ctcgtcgcgc aggcggaagg ccgtggcgac cagcatggcg gcgccgacga cgttctcgag
     1141 gcgcgggtcg tcgagggcgg cggcaacttc atcggggagc tcgagatcgg cgagctcgag
     1201 ctcggggagg gcaggctggt tgtcgttgac ggcggcctga tgagtcatgg cgggctttcc
     1261 cagcagtcgg atgagcgagg gcggatgaag gggagcttct acaaaaaaag gggcccccgg
     1321 gaggcggtgc ctgccggggg ccaaggggag ggaggcaccg tctgacttga cgagtcggaa
     1381 gctaacttaa cctatggttg agagcaacaa taaaatcgcc gaattcgtcg ggtatcgatt
     1441 gcgctttgac acgaaattcg tatgaatttt aacgaagttc gagactgatg atttagttta
     1501 gtcttcacat caaatgacga taaaaatatt cgtaaattca gtcggctgga gcgatcgatg
     1561 gacgaatggc ggcatgcggg gcggtcggtc ggcagccgtt gcatgcgcgg agtttgtcga
     1621 tgatccgcaa ggggttgcag cgccttcatc tgttcgcgca tgagttgaga gatgggcgcg
     1681 cgtcggcctg tcggtggcgc ctcggcccgg gtgtttcttg gcgccggtcg ccaactgcgc
     1741 tagagcgtgg cgcaacgcgc ggcaacgcct gccgccgacc ttttccagcg tagcaaggac
     1801 gggccatggc tggccattca cagttcaaga acatcatgca ccgcaagggc cgccaggatg
     1861 cggtccgcgc caagctcttc acccggctga cccgcgaggt ccaggtggcc tcgcgcatgg
     1921 ggctgcccga tcccgcgttc aaccccaggc tgcgcgctgc catccaggcg gccaaggcgg
     1981 ccaatgttcc caaggacaat ctcgagcggg cgatcaagaa gggccagggc ggcgacaccg
     2041 agagctatga cgaggtgcgc tacgaggggt acggaccggg cggcgtggcg ttcatcgtcg
     2101 aggctttgac cgacaaccgc aaccggaccg ccagcgaggt ccgggccgcc ttcagccggc
     2161 acggcggcag cctcgccgag accaatgccg tgagcttcca gttcgaccgg atcggccaga
     2221 tccggttcgc cgccgaggtg gccgacgagg aggcgatgct cgaggccgcg atcgaggccg
     2281 gggcggagga ctgccagtcc agcgaggacg gccacgagat cacctgcgcg cccgacgacc
     2341 tggcccaggt gaccgaggcg ctggaagcga ggttcggcac gcccgaggcg tcgctgctga
     2401 cctggcggcc gcaggtcacg gtgccggtcg aggacgacaa ggcgccgacg ctcttcaagc
     2461 tgctcgaggt gctggacgag agcgacgacg tgcagcgcgt cgtggccaac ttcgaggtct
     2521 ccgacgaagc catggcgaac ctgcaggcgt gatccgcctc ttgggcctcg atccgggatt
     2581 gcagcgcacc ggctggggcg tgatcacggt gaacgggccc gccctcggct tcgtcgccgc
     2641 cggggtcgtg gccagcgttt cgagcgaaac gctggccgag cggctcctgg cgctggaccg
     2701 gggcatcgcc ttggtgatcg agcagcatgt gccggacgag gtcgcggtcg aggagacggt
     2761 ggtcaacaag aacgcgctga gctcgctcaa gctcggccat gcccggggcg tggcgctgct
     2821 gaccgcggcc cgggccggcc tgccggtcgc cgagtatgcg gcgaagcacg tcaagcgcgc
     2881 gctgaccggc accggcggtg ccctgaagcc gcaggtggcg cacatggtcc aggtgctcct
     2941 gccgcgggcc gggccggtga cggcggatgc ggccgacgcg ctggccgtcg ccatctgcca
     3001 tgcccacaac cgcatgaccc tggcgcgcat ccatggcgcc gtcatggcga ccgtgcgatg
     3061 atcgcgctgc tgcgcggccg gatcgccgcc cagggcgagg accacgtggt gatcgacacc
     3121 ggcggcgagg cgggcggggt cggctacctc gtccagtgcg ccggcaagac gctgcaccgc
     3181 ctgccgccca ccggccagcg ggcggagctg cggatcatca cccaggtgcg cgaggacagc
     3241 atcacgctct acggcttcct gacgccggtc gaggagcagt ggttccgcat cctgcagggc
     3301 atccaggggg tgggcgccag gctggcgctc gccgtgctct cggtgctcga gccggaggag
     3361 atcggccgcg ccatcgccgc ccaggatgcc cgggccctga cccgggccgc cggcgtcggg
     3421 ccgaagctcg ccgggcggat cgtcgccgag ctgaaggacc gggtggcgaa gctgccggca
     3481 gcgctcgcca tcggcggtgc cgcgaggccg gcaccggcgc cggccggcgg cgtggtcgag
     3541 gatgcgctgg cggcgctgca gaacctgggc tacggccgga gcgaggccta tgcggtggtg
     3601 gcgcgggtcc agaacgagct cggaatcgac gccacgctcg agcagctcct cgccgccagc
     3661 ctcaagggcc tcgcccgatg agcgggccgc agggcgagga aggtcggctg gtcggtggcg
     3721 cactgcaggc ggcggacgcc gatgtctcgc ttcggcccaa gcggctcgcc gagttcatcg
     3781 gccaggccca gctcaagagc aatctcgccg tcttcgtcga ggccgcccgg tcgcgcggcg
     3841 aggccatgga ccacgtgctc ttcgccggcc cgccagggct cggcaagacg accctggcgc
     3901 agatcgtggc ggccgagctc ggtgtcggct ttcgcatgac ctcggggccg atcatccagc
     3961 gggcgggcga tctggcggcg ctgctgacca acctgcagcc gcgcgacgtg ctcttcatcg
     4021 acgagatcca ccgcctcagc ccgacggtcg aggaggtgct ctacccggcg atggaggact
     4081 tccagctcga cctgatgatc ggcgaggggc cggcggcgcg ctcggtccgg atcgacctcg
     4141 cccccttcac cctggtcggc gccaccaccc ggacggggct cctgaccacg ccgctccgcg
     4201 accgcttcgg catcctggca cggatggagt tctacgggca ggcggagctg ctcgagatcg
     4261 tcgagcgggg tgcccggctg ctgcggctcg ggctcaccgg cgagggggcc gccgagatcg
     4321 cccgccggtc gcgcggcacg ccgcgcgtgg cgacccgcct gctgcggcgg gtgcgcgact
     4381 tcgccaccgt cgagcagccc aatggggcga gcccggtcga ccgcatccgg gcggatgcgg
     4441 cactgcagcg cctcgctgtc gaccggctcg ggctcgatgc cctcgaccgc cgctacctca
     4501 acgccatcgc cgtgcattac ggcggtggcc cggtcggcgt ggagaccctg gcggcggccc
     4561 tctcggagca gcgcgacacg ctggaggaga cggtcgagcc ctatctcctg cagcagggcc
     4621 tcctgcagcg cacgccgcgc ggccggctcc tgacccaggc gagctggcgc catctcggcc
     4681 tcgccatgcc ggccagcgcc gtcgcccagg ccgagctctt cgccgggccg gacgatgccg
     4741 gcgagggcga gccttgagcg ccgcgcatcg attgccggtt cgggtctact acgaggacac
     4801 ggatgccggc ggcatcgtct atcacgcccg ctacctctgc ttcgccgagc gggcgcgcac
     4861 ggagatgctg cgctcgctcg gggtcgagca gcgggtgctg cgcgacgagg cggacggcgt
     4921 cttcgccgtg cgccgggcgg tggccgagtt cctgcgcccg gcgcggctcg acgacgcgct
     4981 ggtcgtcgag accgagctcc gccatgtccg cggggcgcgg ctcggcctgc tgcagaacat
     5041 cacgcgcggc gggcaagttc ttgtttccat taaggtcgag atcgtcttca tcaactccga
     5101 cttgcgcccg cgccgcttac cgccttggct cgccctgcgt tttgccgagg ccggccattc
     5161 ggttgctggc gaagcttgac aagcgtcgcg ggcgtggccg atagcacgct cggccaaggc
     5221 aaccgccccg gcaggccacc ggcgacgggc cgccacggat caccgccacc atatccgggc
     5281 ctgccggccc gccgtgtcgc ttcccctccg cgaccaacgc gattgaccgc atggaatccg
     5341 atcccatcag ctccgcgatg ctcggcgaac cgaccgccgg catgagcatt ctggctctgg
     5401 cgctcgaagc cgacatcgtt gtcaagttcg tcatgctcct tctcctcgtg gcctcgctct
     5461 gggtttgggc cgtggtcttc gagaagggct ttcgcctggt ccggctgcgg cgccagacgg
     5521 cgctgttcga gcaggccttc tggtcgggca cgcccctggg cgagctctat cgccggctcg
     5581 gcaagcgcat cgaccacccc atggcgctga tgttcgcggc cggcatggag gagtggcggg
     5641 gcgggcccaa ggcggtcagc ggcgacgccc aggaccggct ggtcggccgc atcggccagg
     5701 tcatgcagct cacgctggag cgcgagatgc gcgggctcga gcgcaatgtc tcgacgctcg
     5761 cgaccatcgg ctcggcggcg ccgttcgtcg gcctcttcgg cacggtctgg ggcatcatga
     5821 acagcttcca gtcgatcgcc atgaccaaga acaccacgct cgccgtggtc gccccgggca
     5881 tcgccgaggc gcttttcgcc accgccctcg ggctgctcgc cgccattccc gcggtcatcg
     5941 cctacaacaa gatctcgacg tcggtcgacg ggctcggcga ccgtctcgcc agcttcgccg
     6001 acgagttcgg cgtcgtggtc agccgcgagc tcgacagcag ccgggtcgtg ccctgatggc
     6061 ggcgacggcg gttggcggca cgggtggccg gggcggcaag tggcgccgcg gccggcagcg
     6121 gcgacagatg ggcgagatca acgtcacgcc cttcgtcgac gtgatgctgg tgctgctcat
     6181 cgtcttcatg gtgaccgcac cgatgctgac catgggggtc tcggtcgatc tgccgcgcgc
     6241 cgacagctcg cccatgcccg gccaggacga gccgctctcg gtcagcatcg atgcctcggg
     6301 caccatcttc ctgcaggaga ccgcgatcga gctcgaccag ctcgcacccc ggctgctcgc
     6361 gatcaccgag cagaacgccg aggcgcgcat cttcgtgcgc ggcgaccggg cgatcgacta
     6421 cgggcgggtg atggcggtga tcggcaacct caaccgggcc ggcttcacca aggtggcgct
     6481 gctcaccgag cagccggacc tcgtggtggc cgagtgagcg catgaaccgg tcgttcatcg
     6541 cctccgttct cttccacctc gtcttcatcg gcctcgccgt gctcggcctg ccgcggatgg
     6601 ccgagcgcct gccgatcgac gagccgttca cggtcgagat cgtcggcatc ccggcggccc
     6661 cggagccgac ggaggagccg ccggccccac cgccggccga ggcggcgccg ctgccgccgc
     6721 gccaggcggc gagcccggcg ccacccccgc ccgcacccga gcccgagccg gagccggagc
     6781 cagcgccgga gccgccccag cagctggcgg cacccgagcc gccgccacct ccgccggcac
     6841 ccgagccgcc acccccgccg gcgccggagc caccgccgcc tccgccaccc ccgccggccg
     6901 ccgagccggt gccggcccag gccgccgcac cggtgccgcc ggcgcggccg cgcgcccggc
     6961 cggagccgcc gaagccggtc gagaccgcag cccccgcacc cgagccgccg gcccccgagc
     7021 cgcccaaggt cgagccgccg gcggtgaagg tggcggcccg gccgaagccg ccggagaagc
     7081 cggcgccgcc gaagaaggcg gagccgagcc cggcgccggt ggcgaaggac gacaacgact
     7141 tcaacgcgct cttgcgcagc gtcgagcagc agaaccggcg cgtcgaggcg cccgagcggc
     7201 ggcccggcac cggcaacgcc gaagccgcgc gaacggcccc gccggcggcg atggccgcac
     7261 ccgtggccct ctcggcgagc gagctggccg gcatccgccg gcagatcgag cgctgctgga
     7321 acgtgccgat cggcgtgccg ggcatcgagg ccatgcgggt gagtctcagg atccagctcg
     7381 aggccgatgg ctcggtgcgc gaggtgagca tcgaggaaag cggccgcttc gacgccgatc
     7441 acggctttcg gacggtggcc gagagtgcca ggcgggcggt gctgagctgc cgcctcgacc
     7501 tgccggccga gaagtacgcg gtctggcgcg acatggtttt gacgttctct cccggggatg
     7561 cgctgagtgg ctgatcactt gaaggaccgg cggccgggcg ggccgacgcg acgggcgatg
     7621 ctggccggcg ccacggccgg cctcgggctc ctcgccatgg ccggtggggc cgccgcccag
     7681 gtgcggctcg acatcaccaa gggtgtcgtc gagcccattc cgatcgcggt cagcccgttc
     7741 gctggcgaag atgtcggcac gcgcgacatc ggccgggccg tggccgacgt tatcgctgcc
     7801 aatctcgaga gctcggggct ctttagggtg atcgaccggc gggcctacat ccaggacccg
     7861 gagagcctgc gcgagacgcc gcgcttcgcc gaatggcggc cgatcaatgc ccaggcgctg
     7921 gtcaccggtg tcgtctcgcg gcccgccgcc gaccggctgg tgatcgagtt ccggctctgg
     7981 gacgtcttca gcggcagcca gctgcgcggc ctgcgctacg agctggcggc cgatggctgg
     8041 cgccgggtgg cgcaccagat ctcggacgtc acctacgagc ggctgaccgg ggaatccggg
     8101 tatttcgaca cccgcatcgt ctatgtcagc gagagcgggc cgcgcacgca gcgggtcaag
     8161 cgcctcgccg tcatggacca ggacggccac aaccaccgct tcctctccga tggcggccag
     8221 ctcgtgctga cgccgcagat cgccccggac ggccggcgcg tcgcctattt cgtgctgcgc
     8281 acgcccgtgc cgcagatcat gatgctcgat ctggcgaccg gccgctcggc cccactcggc
     8341 gagttcccgg gaatgtcgtt cacgccgcgc ttcgcccccg atggcagtgt cctgctgctc
     8401 acggtggccc gggacggcaa ttccgacatc ttctcgttcg acacggcgac acggcgcacc
     8461 acccagctca cccagtcacc ggcgatcgac acctcgccga gcttcagccc caggggcgat
     8521 cgcatcgtct tcaacagcga ccggggcggc acgccacagc tctacaccat gaaccgcgac
     8581 ggctcgaacg tgcagcgcat cagcttcggc accggccgct acggcagccc ggcctggtcg
     8641 ccacgcggcg atctcatcgc cttcaccaag atccagggcg gctatttcca catcggcatc
     8701 atggagcccg atggcgccaa cgagcggctc ctgacccgga gcttcctcga cgagagcccg
     8761 gcctgggcgc cgaacgggcg gatcatcgtc ttcacccgcg aggatcccag gaccgaccgc
     8821 tcgcgtctca tgacgatcga catcaccggc tacaacgagc gcgagctgcc gacgccgctc
     8881 gatgcatccg acccggcctg gtccggcttg attccttgag gccggccacg tatccacggg
     8941 aagccgccgt gccgcagggg cggtcccgtc catcgtcgcc aatgttcccg gcgcccgcgc
     9001 caaaccctcc tgccacgagg tgtcagccca tgagaccgct tttcgccatc ctgccggctg
     9061 ctctgctcct gctcgccgcc tgcagctcca cgcccgagga tacgctcggc agtgacgagc
     9121 aggcgggggg cgccacctcc agcgtcgacg gtgcctacgg tgccggcggt gccccgaccg
     9181 agctgggcga cggcagctcc acggtgatcg tcgaggagga tctcttcgcc agcggtgccc
     9241 ccggcagcca gcaggacttc gagatcaatg tcggcgatcg ggtgttcttc ggcttcgaca
     9301 gcacgacgct cgacgacgat gcccgcgcca cgctcgaccg gcaggcgtcc tggctcttcc
     9361 agtacccggc gacgtccgtg accatcgagg gccatgccga cgagcgcggc acgcgcgagt
     9421 acaatctcgc cctgggcgag cggcgcgcca ccgcggtcaa gaactacctc gtagcactcg
     9481 gcgtcacgcc ggaccggctg ctcaccatct cctacggcga ggagcgcccg gtcgaccccg
     9541 gccgcgacga gaccgcctac gcgcagaatc gccgcgcggt caccgtcatc aacctcgtca
     9601 actagccggc ccggctcctg ccgatggcgg ccgggcacga ggctcggccg ccggcttgtc
     9661 cggcctgaag cgggagacct tcgccatcat gtccctgcgt ctcgctgtct cgctcgccgc
     9721 ctgcctcgtc ggctttacac cggccgtggc ccaggatccg caaccgttgc gcatcgagcg
     9781 ggccgccccg gcgccggcgc cgctccagca accgggtgcg gcggcgcccg cggggcaggc
     9841 ggtcgacggg cgggcgatgg cggtgatgca gaaccggatc gccgcgctcg aggaggaggt
     9901 gcggcgcctg accgggcggc tcgaggaggc cgagttccag gaccagcgca cgcgccagcg
     9961 gatcgacgat ctggtcgccg atctcgatcg gcggctgatg gcgctcgatc aggcggcgat
    10021 ggcgcagacc gcgccggagg gccgggcggc acccccgccc gccgacgatg ccgggtcggt
    10081 cgggccggca ctggttgcac ctggggcagc cggcggtgat gcggcggcgg cagatgtcga
    10141 gcccgacccg gcggcgcgcg aaggtcatgt gctcggcacc atcccgcgcg atgcggtgct
    10201 cggcctgccg cagcccgatg gcagcggccc gtccggcacg gcgccgcgcg ccaccctcga
    10261 ggggacggcc gccgcgcgct tcaccgcctc gctcgatctc ctgcagaccg gcgactggag
    10321 cgccgccgag gtcgccttcg accgctggct ggcggatttc ccggatgacc cgcaggcgcc
    10381 cgtggccgcc tactggctgg gcgaatcgca gctggcacgg gaggatttcg ccaccgccgc
    10441 cgcgaccttc gccggcaact accggaccta tggcgaccag gcaccgcgcg cgcccgacaa
    10501 cctgctcaag ctcggcacgg cgctggccgc gatgggcgat cagtcgcgcg cctgccagac
    10561 cttcatcgag atggagcggc gctatcccaa cgcgtcgccg gcactcctgc aggccaggga
    10621 gcgtgagagc cgcgccgccg gctgcggctg agtcttccgg cgagcgggcg ccgctgagcg
    10681 ataccgagtt cgcggcgctg atggccgcac tcggcccctt cgagccccgc ccggcaatcg
    10741 ccgccggcgt ctcgggcggg ccggacagca tggccctcgc cctcttgctg cgggattggg
    10801 tggcgagccg cgacgggcgt cttctggcac tggtggtcga tcacggcctg cgcccgggat
    10861 ccgccgacga ggcggcgctg gtggtcgagc gtctcgcggc actcggcatc gcagccctgg
    10921 tgctccgggt cacgggcgac tggccatccg ggtccatcca ggccaccgcg cgagcccggc
    10981 gctacgcgct gctcgaggca gcggccgagc gggccggcat gctgcacctg gtggtcgcgc
    11041 atcatgctgc cgaccagcgc gagaccgtgg cgatgcgggc ggccgccggc agtggcccgc
    11101 gcgggctcgc cggcatggcg ccgatccgcg agctttgcgc cgtccgcctc ctgcgacccc
    11161 tcctgccggt cgagccagca cggctgcggg cgctgcttcg ggcccggtcg gtcgactggc
    11221 tcgaggaccc gagcaatcgc gacccgcgct tctggcgggc gcgggcccgg gcggcgggcg
    11281 agctgccggc ccttgtgaac gacacgaccg aacgtgccgc cctggagcat cggctcgccc
    11341 gtttcctggc cggccatgcg caggtgcacc cggcgggctt cgtcgagctg tctctggatg
    11401 cgctttcggc cctggcggca gacgagcgcc gccagctcct cggccggctg gtcgcggcgg
    11461 tcggcgggcg ggccttcgcc ccgcgcggtc gctcgctcgc ccgcctcgac gcctggctct
    11521 gcgacccttc gtcgccgcgc tgctcgctcg gcggcacgct ggtcgagcgg cgccggacgc
    11581 gcctgcgggt cctgcgcgag gcccgcggcc tgccggccga gctggcactg gcaggcggcc
    11641 gcacgatctg ggacggccgg tttcgcatcg actggcttgg cgcggaggcg accgcgggcg
    11701 ggccgccgag cctgcgcgcg gccgggcagg gcgggggcgg agcccgggcc ctgcgcgcct
    11761 ggctcgccgc cgtcggcgcc cccgagccgg cagcggcgct gccggccctg gtcctcgcga
    11821 ccctgccgct cgtttcgcgc gatgcgcaag tcctggcgct cggcccctgg gtggcaggcg
    11881 ccgcgcgcgg cgttctggct gtggtctttc gcccgacgca taccatgacg gcggtcggtt
    11941 ccgcaccttc ggtctgttgc gccgacgaac aattgactta tcttgggtaa accataggga
    12001 agcatcgtcg cttcgacctt tcccggcacc ttgccggtcg aggtgaacgg agtggccgct
    12061 ttccaacgaa cgccagttgc gaggctctca cgtgaacaat ttcagcaaga acctggcctt
    12121 gtggatcatc atcggcctgt tgctgatagc gcttttcaat ctgtttcagg gtccgtcgtc
    12181 gcgtggcccg cagacctcca tggcgttctc cgatttcctt tcggaggtcg aggccggccg
    12241 ggtcaacgag gtcaccatca agggggattc gatctccggc acgttcgccg acggtcgcca
    12301 gttctccacc tatgcgccct tcgacccgaa cctcgtcgac cggctgaccg ccaacgacgt
    12361 gcggatcagc gcccagccga acgacgacaa catgccgtcg ctgctcggcg tgctggtttc
    12421 ctggttcccg atgctgctgc tgatcggtgt ctggatcttc ttcatgcgcc agatgcagtc
    12481 gggtggcggc aaggccatgg gcttcggcaa gagccgggcg cggctgctga ccgagaagca
    12541 cggccgggtc accttctccg acgtcgccgg catcgacgag gccaaggagg agctgcagga
    12601 gatcgtcgag ttcctcaaga acccgcagaa gttccagcag gtcggcggcc gcatccccaa
    12661 gggctgcctg ctcgtcggcc cgcccgggac cggcaagacg ctgctcgccc gcgccatcgc
    12721 cggcgaggcc aacgtgccct tcttcaccat ctccggctcg gacttcgtcg agatgttcgt
    12781 cggcgtcggc gccagccggg tgcgcgacat gttcgagcag gcgaagaagc acgcgccctg
    12841 catcgtcttc atcgacgaga tcgacgcggt cggccggcat cgtggtgccg ggctcggcgg
    12901 cggcaacgac gagcgcgagc agaccctgaa ccagctcctg gtcgagatgg acggcttcga
    12961 ggccaacgag ggggtcatcc tgatcgccgc gaccaaccgc ccggacgtgc tcgaccccgc
    13021 cctcctgcgg ccaggccgct tcgaccgcca ggtcgtggtc tccaacccgg acgtgctcgg
    13081 ccgcgagaag atcctgaacg tgcacgtcaa gaaggtccgg gtcgggccgg acgtcgacac
    13141 gcgcgtcatc gcccggggca cgcccggctt ctccggcgcc gacctcgcca acatcgtcaa
    13201 cgaggcggcc ctgctcgcgg cgcggcgcgg caagcgcatg gtcaccatgg acgatttcga
    13261 gtcggccaag gacaaggtca tgatgggcgc cgagcggcgc tcgatggtga tgaccgagga
    13321 ggagaagaag ctcaccgcgt ttcacgagtc cggtcatgcc gtgatggcct actactcgcc
    13381 ggcctccgat cccatccaca aggcgacgat catcccgcgc ggccgcgccc tcggcatggt
    13441 catgcgcctg ccgctcggcg accggatctc ggtcagccgc gagaagctca aggccgacat
    13501 cgccgtcgcc atgggtggcc gggtcgccga ggagcaggtc ttcggccacg agaaggtcac
    13561 ggcgggtgcg tcgtccgaca tccagcaggc gaccaagatc gcgcgctaca tggtgaccca
    13621 gtgggggatg agcgacctgc tcgggcccat cggctacgac gagaaccagc aggaggtgtt
    13681 cctcggccac tcggtgacgc agtcgaagaa cgtctccgag gccaccgcgc agcagatcga
    13741 cggcgagatc cggcggatcg tcgaggaggg ctatcacctg gcccaggaga ccctgcgcga
    13801 ccgggccgac gacctgcacg ccatcgccaa cgcgctgctc gagcacgaga cgctgtcggg
    13861 cgaggagatc accgccatcc tcaagggcga gccgatgccg gctccgagga aggccggcga
    13921 cgatgccgca tcgtccccgc cctcgggccg gcgtggctcg gtgccgacga cccggccggc
    13981 cgatgacggc ccgtcgggcg acccggcgcc ccagcccggc gcctgagcgg cgccagacga
    14041 caacactcct gcagcttcag acgccggcct tcgggccggc gtccttgctt ccgggtggac
    14101 ggtagcggcg ctcgagcagc cggaagacga agggtgcggt catgacgacg atggcgatcc
    14161 gcacgatgtg atgggtcgcg acgaaggccg gatcgacgcc gagggcgagg gcgatgacgc
    14221 tcatctcggc gaccccgccc ggcaccaggg cgaggagcag cagcaggggc tcggtgccgg
    14281 tggcgagatg gaccagagcc gccaccacga aggatgccgc gaacatcacg agcgtgaggc
    14341 cgatgccggc gatcatcgtc gccagcacgt cgtgcagcct gaagccgacg aagcgggcgc
    14401 cgatcgagca gccgatgacg agctgggcga tggcgacgaa gagcactggc ggtgccccct
    14461 cgagcaggcc ggtgagatgg ccgatggcgc tgaccaccat gccgccgagc agggtggggg
    14521 ccggcagcct gagcatggtg ccgagccagc cgccgattcc cgccgcgagc agcaggcccg
    14581 cgacctcgag ccagccggct ctcgctgccg ccggcagcgc cgacggcagc tcgatgccga
    14641 aggcgcggac caggagcggc acggtgagca cgatcagcag cacccggacg gtgtgaacca
    14701 gggcaatgcg ccgggcgtcg ccgccggccc ggtcgcccag gaggaccatc tcgccgaagc
    14761 cgcccggcat gccggcgaag aacgtggtcc ttggatcgaa gcgggtgacc agcctgagat
    14821 aggtcatgct ggagacggcc acgagggcga catagatcgg caggacgagc agcgtcggca
    14881 gccaggtcca gagattggcg acgagctcgg gcgtgaagcc gctgccgagc agcacgccga
    14941 tgatcgccat catggggtcg cgcagggtca ccggcaccgc gaccggcagc cgggtcaggg
    15001 cggcgagggt ggtggcgatc atcggcccca tcatccaggg cagcggccag ccgagccacg
    15061 agaagaggag gccaccggcg aagccgatgg ccaaggcgag accgaggccc cgccattggg
    15121 cggcggtgag agggttgcgc acccgcccgc tcaggcgtcg agccccgcca tcctcgtgat
    15181 cagcgcccag tgggcgtcgc tcaccggcat gaccgaaagc cgcgactggc gcaccagcgc
    15241 caggtcggcg agcgccgggt cggccttgat cgccttcagg gacacctccc ggggcagcgg
    15301 ccgcacgggc gccacatcga cgcgcaccca cttgccggcc gggtcatccg gatcggcata
    15361 ggccgcgcgg gtgatctcca taatgccgat gacggcgggg tcgaccagcg agcggtagaa
    15421 gaaggcgcgg tcgcccttct gcatggcccg catgttggcg gccgcctggt ggttgcgcac
    15481 gccgtcccag ccggtcccgc cctcggcgac cagccggtcc cagctgtagt cctcgggctc
    15541 ggtcttcatc agccactgcg ccatgtcagc ggctccgggg ggctggctgg cccaggcagg
    15601 cggtctcgta ttccaaggcg ggctctcccg atgtgcttgg cttgctgggg tgccggttgc
    15661 tgctaggctc tggggctgtg aagcgcaaga tgccggggcc tttcgccgcc cctgtgcgac
    15721 cggctgcgct gtggcgttta cttggcggcg acgggttgat agaagcgcgc cagtcaccgc
    15781 cacccagcct cgataggccc ctttgatgaa actgctcgcc ggcaacagca atcggccgct
    15841 ggccgaggcg attgcggcct atctgcgcgt cgacctgacc aaggccacgg tgcatcgctt
    15901 cgccgacatg gaagtcttcg tggagatcca cgagaacgtg cgcggtgagg acgtcttcat
    15961 catccagtcg acctcgtatc cggcgaacga caacctgatg gagctcctgg tctgcatcga
    16021 tgcgctcagg cgcgcgtcgg cgcggcgcat caccgcggtc atcccctatt tcggctatgc
    16081 gcggcaggac cgcaagcccg gcccccggac gccgatctcg gccaagctcg tggccaatct
    16141 gatcaccgaa gccggggcga acagggtgct gacgctcgag ctgcatgccg gccagatcca
    16201 gggcttcttc gacatccccg tcgacaacct ctatggcgcg cccctgatgg gccccgacgt
    16261 gcagagcaat ttcggcacgg acgacgtggt catcgtttca ccggatgtcg gcggcgtggc
    16321 ccgggcgcgc gcctacgcca agcggcttga cgccaatctg gcgattatcg ataagcgccg
    16381 cgagcgggcc gggatctccg aggtgatgaa cgtcatcggc gaggtcgacg gtcgccactg
    16441 cgtgcttgtc gacgacatcg ccgacagcgg cggcaccttg tgcaatgccg ccgacgcgct
    16501 gctcgccgaa ggcgcggtgt cggtccaggc ctacgtcacg cacggcgtgc tgtcggcggg
    16561 ggcggtcgcc aggattgccg cgagcaagct cgaccggctg gtgatcaccg acagcatccg
    16621 gccgaccgag ggtgtcaggg tgaccgagaa gatccgggtg ctctccgtgg cccagctgat
    16681 gggcgaggcg atcagccgga tcgccagcga aagctcggtg tcgagcctgt tcgactagcg
    16741 gcgccgcacc atggtgcgag tgttcaacca gaaatgacct gtccggggcg gcaatcccgc
    16801 gcgtgccgga ggggtgaaag ggaagaccga tgagcgagac catccagttg acggcggagc
    16861 cgcgcgagcg gaccggcaag gaaggggcgc gcgcgctccg caagaccggc atggtgccgg
    16921 ccatcgtcta tggcggcaag gagccgccgc agaaggtggc gctggtcgac cgggagatcc
    16981 gccgccagct cgagctgaac ccgcgcttct tcagctcggt cgtcgagctc aagctgggca
    17041 agaaggtgat ccaggtgatc ccgcgcgagg cgcagctcca tccggtgaag gaccaccccc
    17101 tgcacgtcga tttcctccgc gccgccaagg gctcgaccgt gaccgtcgag gtgccggtgc
    17161 atttcctcaa cgaggagcgc tgcccgggtc tccggcgcgg cggcgtgctc aacatcgtgc
    17221 ggcgcgagat cgagctggtc tgcccggtcg aggcgatccc cgagcgcatc gacgtcgacc
    17281 tcgccgccgt cgagatcggc gattccgtgc atatcagcgc cgtcaccctg ccggccaacg
    17341 tgacgccgac catcaccgac cgtgacttca cgatctgcgc cgtggtcggc cgcggcccga
    17401 ccgccgacga ggacgaggaa ggcgagggcg agggggccga ggcgggcggc gaggccgacg
    17461 actgagcccg ccaggctcgg gcaccggcgg gatgcggctg ctcgtcgggc tgggcaatcc
    17521 cggcgccgcc tatgccgggc agcggcacaa tgtcggcttc atggcagtgg aagcggtggc
    17581 cgagcgccac cgcttccccg cctggcggcg caagttccag gccctggtca ccgaggggcc
    17641 gatcggcggc gagcgggtca tgctgctgaa gccgcaaacc tacatgaacg aatccggtcg
    17701 ggcggtcggc gaggccatgc gcttcctgaa gctgccgctc gacgacctcg tcgtgctgca
    17761 cgacgagctc gacctggcgc cgggccggct cagggtgaag cagggcggcg gcgtcgccgg
    17821 ccacaacggc ctgcgcagcc tcgacgccca tctcggcagc cgcgacttca agcgggtgcg
    17881 gatcggcatc ggccatccgg gccacaagga ccgggtgacc ggccacgtcc tcggcgactt
    17941 tcagaaggcc gaccggccgt ggctcgaggc gatgctggat ggcatcgcgg tcgccgcccc
    18001 gttgctcgtg gctggcgacg acgcgggctt catgaaccgc gtcgccctcg agatgcagcc
    18061 ggtgctcaag aacggtcagg gcggcgtgta ggcggcgccg cgcggcccgt cgaggaagcc
    18121 gttcgcgaaa cgggcatccg gcagcggcgc cgcgggcgcg gccggcctcg ccatccaacc
    18181 ttcgacggcg gggctcggcc agcgccacca tgtcgacggt gtgcggcgac aggcggaagg
    18241 agccgacgcc ggcggcagtc agccggtcga gatcgccgat caggctggca tgggcgaagg
    18301 acagggtctg caccccgttg accgcgagaa agcgctcgcc gtcgagcgtg ccgtgcagca
    18361 tgccgagaag ggcggcgagc gatccgctga gccgtgcgtc gaaggcgggc aggcggcctc
    18421 gcccgaacgg cacgatggcc gagcgcccgg cgccgggacg cgcgagaaaa acgaaaggaa
    18481 catcggtcgg atcgacgaga aagcgccttt cggcatgctc gccctgttcg gcgagcgtgc
    18541 cggtgcgtcg gggcgatcat cggccggcgc acccgcctgc cgcggtcgcg gcgtcgggcg
    18601 cggcggcccg ccgggtcgcc agcacgaagc ccgtgaccag cacgagctcg aggccggcgg
    18661 cggcggcgcc ggcggctccg gccgcgacga ggccgaagtg ctgcatcagg gcgaggaggg
    18721 ccaggagctg ggcgccgcgg ccgatggcct gcgccaggag caggcggccg gcgtggccga
    18781 gcatgaccag caccgaggcg gacggcatca ccagcgccag aagcgcggtc tgcagcgcca
    18841 ggaggacgag gaccggatgg gccgcggcga acgcctcgcc gaagaagaag cccaggagcc
    18901 ggtcgccgaa gagcccgagg acgacgagaa gcggggccgc gaagcaggcc gtgcccagaa
    18961 gcgtgcggcg aaacaggcgc cagagcgtcg ccatgtcggc cgctgccacg aggcgggcga
    19021 actcgggatc gaccgcgcgc ctgacgatct gcagcggccg ctcgatgacg gcggccacct
    19081 gataggcgac gaggtagagc cccgccgcga ccgggccgag catggccccg accagcacgg
    19141 tggcgaggcg ccccgagacc atgccgagcg tgccctgcag gttggtccag agcagcatgc
    19201 gccagagccc ctggtgcggg cggacgaggc cgcgcacgcg cagcctgaga tccttcagcc
    19261 agccgccccg gtgcagctcg agatgggcga ggcgccagag cagcagcgcc tcggcgctgc
    19321 cggcgagcgc ccagacggca gcgaacaccg gcaggcccgc cccgccggcc caggcggcga
    19381 ggcagcccac gagacggagc gtcggcgtca ccggccgggt ccaggcgatg agagcgaagc
    19441 gatcgagcag gcgcaggatg ccgatcggcg tggcgccgtt cgaggcgatg acgacgaggc
    19501 agtaccaaag tgctgtctcc cgataggcct cgtcgaggcc gagccgcggg ccgagccagc
    19561 cgagcgccac gacgcccagc gccatcgccg ccgccacgcc ggcgagctcg accccgactc
    19621 cgagggtcag caatccctgg agatcccgcc ggcgctggtc ggcgaggcag ctcgccccgt
    19681 agcggaggat ggcctggtag acgttgaatc ggcaaaggcc acccatgatc atggcatagg
    19741 catgaaagat catgagaacg ccgaacgcct cggcgccgag gccgcgcgcc gccacggtca
    19801 ccgagccgag cgccaggatt cccgcgacac cgttgccgct gagcaacagg ccggtgttgc
    19861 gggcgatccg gcgcaggaga tggtcgtcga agaggcggcg caaggttcct cggtcggcag
    19921 ctggggcaat cgacgatcgg ctaccacggc accgtcgccg gcaaccccat gacggcttgg
    19981 cttgaccacg aattcggcat tccctatgcc gatttccgac gcggccggca agaagcggcc
    20041 gcaagaggcg gcctgcccga tcaacgggat cctcggctcc atgcatatcg ggatgttttt
    20101 ctcggggctc gccggcggcg gcacgcagcg ccgcatgctc gccctggcgc gggggttcgc
    20161 cgcccgtggc catgaggtgg acatcctcgt ggcatctgcc gagggtgctt tccgcgcgga
    20221 ggtgccgccc gatgccggcc tcgtggcact cggcgccgac tggcggcgcc tgcccctgat
    20281 cggccggcac cgcggtctct gggtgccgct cgccgcccgc gccctcggcc ggcggctcag
    20341 ggcggcaccg ccggatgtcc tcctcgccag ctcgacaccg gcgaacctca ccgcttccct
    20401 ggtccgccgc cgcgccgcac cggctgtgcc gctggtcctc accctcaatc tgccgccctc
    20461 ggcggtgagc gggggcctcg gccggggggg ccggccgctc cgcgccttga tgcgccggtg
    20521 ctacgccgag gccgatgcgc tggtcgccat ttcggccggt gtcgccgccg atgccgcggc
    20581 catgctcgac ctgccggccg cgcgcatcgc cgtcgtcatg aacccgatcg acactgcgcg
    20641 catccaggcg ctggcagggc aggaccctgg tcatccctgg ctcgagccca cggccccgcc
    20701 gctcctcctc gccgttggca agctcaaggc gcaaaaggac ttcccgaccg tgctcgaggc
    20761 cttcgcccgg gtccgtgccg tgcgtccggc acggctcgtg gtgctcggcg acggcgagga
    20821 gcggcagtcg ctcgtgcacc tcgcgggccg gctcggcatc gccgccgacg tggccttcat
    20881 gggcttcgtc gccaatcctt tcgcctggat ggcgcgtgcc cgcgtattcg tgctcggctc
    20941 ccgcttcgag ggcttcagca acgtgctggc cgaggcgctg gccgtgggct gcaccatcgt
    21001 cagcaccgac tgcccgcacg gcccgcgcga gctgctcgac gaaggccggc atggccggct
    21061 cgtgccggtg ggcgacgcct cggccatggc cgaggccatg ctcgcggcgc tcgacgatcc
    21121 ggccgatccc ggccgccagc gggcgcgcgc cgcccgcttc tcggtgggtc gcgccgtcga
    21181 tggctatctc gaggtgctga ccgccgtcgc ggcacccgat cggcaccttc gaagggccgg
    21241 ctgatggacc ggcaggagcg gcgtctggcc attctcgttc gggccctcga cggcggtggc
    21301 atgcagcgca acacgaagcg gctggccggc gccttcgccg cgcgcggcca tccggtcgac
    21361 gttctggcca ccgacgtgag cgggccgatc cgccaaggca tcgagagcgt ggcccggctc
    21421 gtggcgctgg cagggggcag ccatgtcacc gggcgccttc tggcgctgcg cgccggctgg
    21481 cgcgagcgca cggcactccg cccgctgctg ctcggcggtg ggccgggcat cctcgcccat
    21541 ctgccggcgc tcgtcgccta tctccgcctg gcccgcccga cggccctgct ggcgctcggc
    21601 acgcagtgca acctcgccgc cctttgggcg cggcgcctcg ccggcacaca cacgcgcgtc
    21661 gtcgtcagcg agcgcaacac cctctcgctg gtggtcgcca acacccgccg ccgctttcgc
    21721 cgcgcctacc cggccctggt cgcgcgcacc tatcccgagg ccgacggcat cgtcgcggtc
    21781 agccgtgccg tggccgacga tctcgcccgg acctgccacc tcgccgccga caccatcacc
    21841 accatcccca atccagtgcc cgagcccgag gacagaggtg ccgacgaccg ggccggaaac
    21901 gccccggccc atccctggct cggcccggat cagccgccgc tcatcctcgg cgtcggccgg
    21961 ctgaactggc agaaggactt cgccacgctg ctcgaggcct tcgccctcgt tcggcggcga
    22021 cggccggcgc ggctggcgat cctcggcgag ggcgaggagc gccagcggct cgaggcccgt
    22081 gcccgggagc tcggcatcgc cgccgacgtc gtcatgccgg gcttcgtcga cgacccgttc
    22141 gcctggatgg cgcgggcccg gctcctggcg ctgacctcga gccaggaagg cttcggcaac
    22201 gtcatcgtcg aggcgctggc ctccggctgc ccggtcgtgg ccagcgacat tcccggtggc
    22261 ccgcgcgaga tcctcgacgg cgggcgctac ggcagcctcg cgccgcccgg cgacgccggg
    22321 gccttcgccg aggccatgct cgccgcaatg gacgacccac cagcgcgggc cctgctcgtc
    22381 ggccgggccg gggactacgc cctcggcccg atcgccgaac gctacctcgc tttgctgctc
    22441 gacaaggagc tcgaatgacc atcgacaccg atgatggcgt gttcgccgag gacgccgcgg
    22501 cactgcatgc cctggtgccg tcgacacccg ccaagcacat tgccgtcttc atctacgccc
    22561 tgaccggtgg cggtgcccaa cgccgcaccg tgaccctggc caacggcttc gccgaatgcg
    22621 gccacgatgt cgatctcgtc gtcgtgcacg cccggggcgt catccaccac gagctggcgc
    22681 cgcgcgtgcg gctggtcgat ctcggcgacg gctacggccg gctgcatgcc gcggcctgcc
    22741 gccgtggcgc gccacgcggc ctgcagaccg ctctcgccgt tccggcactc gctcgctacc
    22801 ttcggcgccg ccggccggac gtgctgctgt ccgccgccag ccacgtcaat ctcgtcgccg
    22861 tggcggcggc acggctgtcg cgcaggccca tcccgctcgt ccttcgcgcc agcaaccatc
    22921 cgtcgggcaa tctcgcccac catccgccgg tccagcgcct cgtccgcgcc tggctcaaat
    22981 ggctggcccg ccgcttctat ccccgcgccg atgccatcat cgccgtttcg agcggtgtgg
    23041 ctcgtgagct cggccgcctc acggcgctgc cgccggaacg catcacggtc atcgacaacc
    23101 cggtgctcac cgcccgactg cgccggcgca tggccgagcc gctcgagcat ccctggctgg
    23161 cacccggcag cccacccgtc attctcgccg ccggccgctt cacgctgcag aagggttttc
    23221 cgacgctgct cgaggccttt cgtcggatcg cgcgtgattg cgacgcccgc ctggtcatcc
    23281 tcggcgacgg ccccggtcgc gagaccctcg ccgcgcgggc aaaggcactc ggcctcggcg
    23341 agcgggtgct catgccgggc tatgtcgaca acccgctcgc ctggatgtgc cgtgcgcgcc
    23401 tcttcgtcct ctcctcgctc tgggagggcc tgccgggcgt gctcgtcgag gccctggcct
    23461 gcggcctgcc ggtggtgagc accgcctgcc cgagcggccc ggcggagatc ctcgaggacg
    23521 gtgctcatgg caccctcgtg cccgtcggcg acgccgaagc catgggtcgc gccatcctgg
    23581 cggcgctcgc ggcccggccg gagcccgagc ggctgcgcgc gcgggccgag catttcgccc
    23641 tcgccccgtc gatcaaggcc tatctcgacg tgctcgaggc ggtgatccgt cgccgcgcgc
    23701 ccgagcggct cgcctggtcg ccgccctggc cgctcaccgt gcggctcgac tggacctcgc
    23761 ccgaaggtct gtcccgtgcg ctcggccgcg tctgccgcgc gcccggcgat ctctttcgcg
    23821 cctggccggg caatgccgac caccgccagc gcatggcccg catgatgcag gcattcgcga
    23881 tcgatcccga ccgggcggcc gacacgcgtt ggcaggagct cgtcgctgcc gatgcgcgct
    23941 gcgccggctg cgtgcggatc gcgcgctgcg ccgcctggct ggcacgaccc gggccggccg
    24001 ccgaggccgc gagcttctgt cccaatgccg ggctcttcgg cacgctcgcc gacgaggcgg
    24061 tgagagagcg ggccgcggcc agcgcatgat cgagggcatg gcccggagtt ccggcgcgag
    24121 caccctgccg cgcgggcagg ctcctagcgc cggagctcga gcagggccag gccgagggcg
    24181 agggccagca tggccgccac gacatcgcgg cggctgaccc cgccagtcct ggccatggcc
    24241 cgttcgcggg ccgccgcatc gaccagcgat cgctcgagcc ggcggccgag ggcggtgaag
    24301 cggcccagat agagacgatt ggcgaacgtg ccctggacca acatcgcggc gagctgaaga
    24361 ccgcagagca accagggcga ccagccgtgg cgccagagct cgagccagag caggagccag
    24421 agcacgctcg ccagggcgac caggcgcagt cgccgatagg cgaaccagaa ggggccatag
    24481 agcgcggcgt ggcggttcca gctcgtctgc cgcccgcgcc acgccatttg tcgccaggtc
    24541 tcgtcgtaat agctggcatg cggcccgacg aaagtcctgg ctgcgtcgaa ggcgaagtcg
    24601 gcttgtggtg ccacgacatc gatcgtctga ttgctgtgca tcgggccggc tccacgccac
    24661 atggcttcga ggggcgacag ggggtcgagt gttgaatagt ggtgagcccc cgcccgggtc
    24721 aaaagctgaa tgaccggtta accagagaag ccccggggcc gcgtacgacg gtgctgcccc
    24781 gcctttaacc tcccggcaag gttgccggga ggattcgcac gtcggacgct tgccggcacc
    24841 tcttgaaccg ccctcgcgac tgcccatctc ttgggagccg aaggtcgcac cgcgagttcg
    24901 gcaagtatcg gccaaggcgt gtccgcatgg agcgccgagc ctgtcagtat cgctcaactg
    24961 gaggataccg tgagcactgt ccgtttcgat cccttctttc gcaccgtccg tgacctcgac
    25021 cgcatgaccg acgagctgct cggcagccgc ggcggcttcg cgccggaata cgacatcgag
    25081 gaccagggcg aggaccgttt cgccattgcg ctggccctgc ccggctacga tctcgacgag
    25141 ctcgagatca ccgccaagga ccgccagctc gtggtcaagg gcgagcccaa ggccggcaac
    25201 cgcgccaact acatccgtcg cggcatcgcg cgggcctcgt tccagcgcag cttcacgctc
    25261 ggcgagcata tcgagatcgc gggtgcgtcg ctcgacaagg gcatcctgcg cgtcgagctc
    25321 gagcggcgcg tgcccgaggc gatgaagccg cggaccatcg cgatcgccgc cccggccgct
    25381 ggccagccgg cgccacagcc ggaggccgcg gcggtcgagc aggctgcctg aggcaccacc
    25441 gatcgacgtt gacgagcatc cgaaagcctg gcgggtcgcg gcccgccagg ctagcttcct
    25501 gcccgggcag cggacgggcc ggatgccgga cgaatttccc ctggaaaggg ctggatgagc
    25561 gacgagctct atacgacact cggggtcggc aagacggcgt cggacgacga gattcgcaag
    25621 gcctatcggc gtctggtcaa gacctgtcat cccgacacca acccgaacga caagcgggcg
    25681 gaagagcgct tcaaggcgct cggccgtgcc tatgcgatcc tgggtgatcc ggacaagcgc
    25741 aagcgctacg accgcggcct gatcgacgcg gacggcaacg agcgcgggcc gcagggcttc
    25801 ggcggcggct tcggcggctt cgatccggga cgcgccggcg cgcgtcggac ctaccaccgc
    25861 agtgccggcc accgcagtgc cggtcccaac gccgagttcg gcggctttgc cggcttcgac
    25921 gacgtgctga gcgatctctt cggcggcagg accggccggc gtgccaatgc ccgggcacgc
    25981 ggcgaggatc tgaagctcac cgtcagcgtc gatttcctgg aagccgtcaa gggtggcaag
    26041 cgccgggtcg ggctgcccga cggacggcac ctcgacgtga ccatccccgc cggcatcgag
    26101 aacggccggg tgctgcgtct caagggccag ggcatggccg gtggcgacgc cctgatcgag
    26161 gtccaggtgc ggccgcacga aggctttcgc cgggacgggc tcgatatcca tctcgaccac
    26221 agcataccgc tcgacctcgc gatcgccggt ggccgggtga cggtgccgac catcgacggc
    26281 ccggtggccg tgacggtccc tccctggacc agctccggcc gcacgctccg gctgaagggg
    26341 cgcggcatcg gccagggcgg acgccagggc gatcagctcg tgcggctcga gatcctcctg
    26401 ccggaccgag cggacgagac gctgcagcgc tgggcccggg accgggtcaa ggcgactgcc
    26461 tgaaccaggg tctgggcccg atcacacccc gagcacgtgg acggtgacgc gggcgacagt
    26521 ctcgcggagc atccgggcga gcatcgccac gcgacgggag cccgtgaggc gcccatcccc
    26581 gccagggctc cttgcaggcc tccttgccgt ggccgattcc gacggtccgg ctccctattt
    26641 cagccgagct cggaccctag gcgccgccaa tgaggatgcc cgcggccagc accagcgccc
    26701 cgcccagcac gacctggaag gtcgcccggg cgaagggcgt ctccatgtag cggttctgga
    26761 tccagacgat ggcccagagc tcgatgaaga cgacgatgag ggcgatgaca gtggccgtcc
    26821 agaactcggg gatgagatag ggcagggcgt ggccgaggcc gccgagcgtg gtcatgatgc
    26881 cggatgccag gccgcgcttg atgggcgagc cgcggcccga gagcttcccg tcgtcgtggg
    26941 cggcctcggt gaagcccatc gagatgccgg cgccgatcga cgcggaaagc ccgacgagga
    27001 atgtcgtcca ggtgctctgg gtggcgaagg cggtggcgaa gatcggcgcc agggtcgaga
    27061 ccgagccgtc catcagcccg gcgaggcccg gctgcaccca ggtcaggatg aactggcgcc
    27121 gggcggcgct gtcctcctcg ccgcgcgtgt cgtcgtcgag atggcgcgcc tccagatcac
    27181 ctgccttgcg aacatggccg gcctccgcgg cggcgagatc gccgagcagc ttgcgtgtct
    27241 cggcgtcggc cgtgcggccg gcggcggcga cgtagaaggc gtgcgcctgg cgctccatgg
    27301 cctgggcctc ctcgcggatc cgctcgaggc ccaggttctc caccagccag acaggctttc
    27361 tgctgtagaa atcggccacg tgctcgcggc ggatcggcag gatcagctcg ccgaagcgct
    27421 tgcggtagag ctcgatcagg cgatggcggt ggcggttctc ctcctctgcc atgccgatga
    27481 agacggcggc cgaggccggg taggcgtcct gcagcttctg cgcatagagg gcgtagatgc
    27541 gcccgtcctc ctcctcggcg gaaatcgcca gggccaggat ctcctgctca cttagctggt
    27601 cgaagcgacg cttgccgccg aagcggggca gggggaagag gctgatcatc gaggctccgg
    27661 gtttagatcg attcaaatct agacgatcgg tccggggcgg caagccgcct gatgcggcgc
    27721 ccgctcactt tcgctcgcga ccgcgcggat cgagcttgaa gatgccgggt gaggtgcgga
    27781 tcagcccgac gaaaccctcg cggccggtgg tggggagtcg tggccggcgg ctgatccggt
    27841 agacggtgaa gagcaggaag aagccgtgca cgcccgaggt gtaccagaag agcgcctggg
    27901 gcccgaggag ggccacggca ccggcgccga ggagcggccc gaccatggcg ccgatgccgt
    27961 agacgaagaa gagcccggcc gccacctcga cgaactcgtc gggctgcgcc aggtcgttgg
    28021 cgtgcgccac cgagagcgag tagatcggaa tggcgaagcc gccgaagaga aagccggtga
    28081 gaatgatcgc cgtgggtgag agcccggtcg cgaccgacat ggcgaagctg gtggcgatgg
    28141 cacctgtggt cgtggcgagc agcaccgtgc gccggctgag ccgatccgag agggcgccga
    28201 tcgggaactg catcaccgcc ccgccggcga tgaaggcgct catcagccag gcgatctcgg
    28261 cgatcgtcag accctggtcg agcagcgcca ccggggccac cgtgcggaac gtgctgttga
    28321 ccaggccgac ggagaagacg ccgatggtgc cgagcggcga gacggcgatc agccggccga
    28381 ggcggagcct gggcgtctgc aggggtgccg gcagggtcga tcgggtcatc gccagcggca
    28441 cgaggcagca gcagaagagg atggcgacga gcgagaagag ctcgaagccg cgcgggtcgg
    28501 cgagtgcgag gacgagctgg ccgagggtga cgcaggcgag gtcgacgaga cgatagacgc
    28561 tgagcacgcg gccgcggtcg ctgttgggcg tgcgctcgtt gagccagctc tccagcacca
    28621 gcgtcaggcc ggcgaagcag aagccgccga gcgtgcgcag caggagccag agccagggct
    28681 cggccagcat cgcgtgcagc agggccgcca cggcatacat cgcgccggcc gcggcgaagg
    28741 tgcggatatg cccggcccgg atgatcagcc tgggcgcgta gagcgagccg gcgacgaagc
    28801 cggcatagaa gaccgacccc agaagcccga tcatcgtcgg cgagaaggcc tcgagattgg
    28861 cgcggacggc gatcagcgtg ccgaagagcc cgttgccgcc gagcaggaga ccggccgaga
    28921 cgaagagggc ggtgaaggag gcgaggcgaa ccatggcttc agccctgcgg cgcgggctcg
    28981 cgcacggtga ggaggaggag caggccgccc gccagcagca ggagcacgac aaccgccatg
    29041 ccggcccgct gggtggcgaa gagcgtggtg gcggcaccga agaggaaggg gccgaggaag
    29101 gccgtgagct tgcccgagag ggcgtagagg ccgaacatct cgttgcgctg gcccgcgggc
    29161 gagagccgcg ccatcagcgt gcggctggcc gactgggcgg ggccgacgaa gat
//
LOCUS       DAOTFI010000019        29075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1814_length_29075_cov_6.696968, whole genome shotgun sequence.
ACCESSION   DAOTFI010000019 DAOTFI010000000
VERSION     DAOTFI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 29075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29075
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_29075_cov_6.696968"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /gene="coaD"
                     /locus_tag="P5558_03115"
     CDS             <1..438
                     /gene="coaD"
                     /locus_tag="P5558_03115"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRY23333.1"
                     /translation="RATVLVDELIVAVAINAGKEPLFSLEERTAMVEADINAMMAANE
                     HNGARVTVLPFKNLLMAFAADNDADFIIRGLRAVSDFEYEFQMAANNKRLYPKIETVF
                     LMASETNQFISSRFVKEIHLLGGDVTSFVSEAVMVQLRTKSRD"
     gene            440..1477
                     /gene="queA"
                     /locus_tag="P5558_03120"
     CDS             440..1477
                     /gene="queA"
                     /locus_tag="P5558_03120"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRY23334.1"
                     /translation="MDVDAFDFELPADLIAQRPVEPRDAARLLVIGDNLQDRTVHDLP
                     GLLAPGDLLVLNRTRVLPTRFAARRQRGGRPIQITLVADQGDDRWSAFARPGRHLTPG
                     DTLELASGLVAEVLAKDADGRFALQLQAEDGTVIDAIRAGGSMPLPPYIRRPAAGEAA
                     DRERYQAIFARDEGSVAAPTASLHFTDRLLADLAARRVGHAFLTLHVGLGTFLPVKVA
                     KARDHVMHPERYVLPEATVDAIAATRAAGGRIVAVGTTVLRVLEACARETGELHPGQG
                     STALFITPGFDFRVVDRLLTNFHLPRSTLFMLVAALAGLDRMQAAYAHAIASRYRFFS
                     YGDACLIDRRD"
     gene            complement(1490..3061)
                     /gene="otsB"
                     /locus_tag="P5558_03125"
     CDS             complement(1490..3061)
                     /gene="otsB"
                     /locus_tag="P5558_03125"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRY23335.1"
                     /translation="MSDAAPVLDPSRIDAVILDMDGVLTDTARTHEAAWKTVFDAFLE
                     ARDGRGFAPFTTADYRRHVDGKPRLDGVRDFLASRGIDLPLGGSDDAPDAPTIRALGR
                     RKNQAFLDRVAREGVSAFADAVAFVDRARRADLKVAAISASRNAEAVLTAAGIRPLFQ
                     VLVDGTVATAEGLAGKPAPDVFLAAVEALGVPASRAAIVEDAVAGIEAGCRGGFGLVV
                     GVARDGETAGLAAAGAASVVASLDHLHIKGDRPLRDRAALPDALASIDAIVALAGDRV
                     LHLFLDYDGTLTPIVERPEDAILDETMRQRVRQLAGRHFVAVISGRGLGDLVERVGLR
                     NLFFAGSHGFELRHPDGRLEEHDEAQAAATRLGELGKALAQRLGPIAGVQLERKRFGL
                     AVHYRRVGDAATGDVERAIRDTAAAFPELTVKAGKKVFEFVPALDWDKGKALSWILDS
                     AGRTRERTLPVYIGDDVTDEDAFRAIEGWGIGIAVGQDGPQSTAAHYRLADVAAVGHF
                     LDRLAERLDGRKRSA"
     gene            complement(3058..5457)
                     /gene="ppsA"
                     /locus_tag="P5558_03130"
     CDS             complement(3058..5457)
                     /gene="ppsA"
                     /locus_tag="P5558_03130"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRY23336.1"
                     /translation="MPTEDLLVIELGELRRTDVDRVGGKNASLGEMIANLQAQGVRVP
                     GGFATTAHAYWHYVDANNLRDAIAETMAKLKDDHSNLARVGRAVRELLENAEVPPALA
                     EAVRRAYAAMAEGGEPVSVAVRSSATAEDLPEASFAGQLESYLNVQGEAALLRALRRC
                     YASLFTDRAIAYRVNNRFDHLKVALSVGIQRMVRSDKAGSGVMFSIDTETGFPRNVLI
                     DAAWGLGELVVQGIVDPDEYTVFKPLLEDTALRPIIEKRLGAKDRKMVFTTGKEAVRT
                     VSTARAEQHRFVLEDDDILELARWAVRIERHYGRPMDIEWAKDGLTGELFVVQARPET
                     VQSRRAAGMLKAYHLTGQGEPLVKGLSIGASIGSGPVCKLANPTEIDRFVEGGVLVTG
                     MTDPDWVPIMKKAAAIVTDHGGRTSHAAIVSRELGLTAVVGAGDATAKLADGQEVTVS
                     CAEGDTGFVYAGLLDFEVEEIAIDSIPATTTKVMLNLADPAGAFTWWRLPTDGVGLAR
                     MEFIVENLIRIHPMALVKFDELTDEDARRQIDELTIGYASKPEFFVDRLARGIARIAA
                     AHHPAPVIVRMSDFKTNEYAELIGGRAFEPKEENPMLGWRGASRYYSDGYREGFALEC
                     RAIRRAREELGFTNIVMMIPFVRTVGEADAVLAEMAEHGLMRGRAGLEVFMMCEIPAN
                     IILAEKFAERFDGFSIGSNDLTQLTLGVDRDSSILSSLFDERDPAVEASIATLLAKAR
                     AAGTKTGICGQAPSDHPEFAAFLVEHGIDSISVSPDSFMAVKRIVAEAEASRQKSATA
                     K"
     gene            complement(5447..7903)
                     /locus_tag="P5558_03135"
     CDS             complement(5447..7903)
                     /locus_tag="P5558_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRY23337.1"
                     /translation="MSWSLTYDAYEPDREKLRESLTTLGNGYFCTRGAVTCTGADAVH
                     YPGTYVAGLYNRLTSRIGDRDVENEDLVNVPNWLPLTFRIGDGPWFAIDEVQVLDFVQ
                     QLDTRQGLLHRDFRFRDQAGRTTRWRERRLVSMADRHEAALEVELVAEDWSGEITIRS
                     GIDGSVVNAGVERYKALANRHLETLGTEAFGSDGMLLRCRTAQSRIEVVEAARLGLFR
                     DGERLDRPIEVVQASDQIEQLARVQLDHRPLRIEKLVALYTSRDPAIAEAGLEATESL
                     TRSTGFEALLARHLLAWEQLWGSCEITFDRPEGDGEATHLRVHIFHLLQTISANSVDY
                     DVGVPARGWHGEAYRGHIFWDELFILPFLVLRVPDLVRETIRYRYRRLGQARLAARAE
                     GFKGAMFPWQSGSNGREETQKLHLNPESGRWLPDTSHRQRHVNAAICYNIWRYWEATG
                     DTGFIRHYGAEMFFAIAQFWVSAATYDAAIDRYDIKGVMGPDEFHTAYPGTDPEAQGG
                     IDNNAYTNVMVAWLLTRALDMVAVLPHDCTDELFARLEIAGADLELWYDVSRKLKVPF
                     HGDGIISQFDGYEQLVELDWPAYREKYGNIQRLDRILEAEGKSPNDFKVAKQADVLML
                     FYLFSAEELDLIFEGLGYPFDSAAIRRNIDYYLARTSHGSTLSLIVHAWVLARADREQ
                     SWHLFRRALDSDILDIQGGTTPEGIHTGAMAGTVDLVQRNYLGLETRSGVLHFNPLLP
                     VGVDCLRVCLRYRRHHLEVEATRSRLRVRSRPALAPMIEIAYRSHFRKLSPGDSHEFR
                     LIRPSERKRGNVRGETTDAD"
     gene            8088..9497
                     /gene="glnA"
                     /locus_tag="P5558_03140"
     CDS             8088..9497
                     /gene="glnA"
                     /locus_tag="P5558_03140"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRY23338.1"
                     /translation="MAGAEALLERIRDEGVRSLDLRFTDLAGRWRHTTLDAAGVKPFQ
                     LEHGVLIDGSAVPGWRDVSESDLLLRPDLASAFQDPFAAQPTLCLFADGAEPATGMSY
                     ERDPRSTVARAEAHLLESGIADTLVAAAEIGFFIFDDVRIERGPLKAGYALESSEART
                     AGGAAYAGGNPGHRPAPGAAQACLPPADHHADIRAEITTTLGGLGFTELRHEHGPAAA
                     QHRLMIGKGGLLATADRLQLLKYVAHQVTATYGKSASFMAKPIADEPGAALFLAQSFW
                     QGGKPLFAGQGYADLSPLCLQFTAGILAHGRALNAFTNPTTNSYRRLQPNLDEPTLLT
                     FAAHNRSAAIRIPYADRPDRKRVELRFADASANPYLALTAILMAGLDGIARKLEPGDA
                     MDRNLYDLRAEELEGIPSTCRTLEAALDALDADHEFCLRGEVMSGELIDAYIAVKRAE
                     VEHLARLPTPAEFELYYGL"
     assembly_gap    10336..10435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10436..11555
                     /locus_tag="P5558_03145"
     CDS             <10436..11555
                     /locus_tag="P5558_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY23339.1"
                     /translation="AMLGSGVGAAWAQAAPTPVTVVTLKAQDLTLTSTLPGRVVASGV
                     AEVRPQVDGIIVERLYEEGAHVKVGDLLYRIDSATYEAQVAAAEAQVAQAQARLTSAE
                     KDADRVRALVDRRATSQQALDDAVAERDAADAGLKVAQAELLAAQIDLDRTTIRAPLS
                     GIIGRSLTTQGSLVTAGQAAPLAIIRTIDPVLVDVTQSAAEIIAWRRGATAERLADAQ
                     QTVTLTLADGKPYEETGLLTAAEPYVHEQTGVVTLRLEFPNPTELLLPGMYVQVELPQ
                     AVARNVILAPQEGVMRDHRGRPIAYVVNGDDVVEERVLAVLQAHGAFWVTADGLADGD
                     RIVVAGLQKIRPGATVAPAEREAAPDATSANLADSNGG"
     gene            11577..14708
                     /locus_tag="P5558_03150"
     CDS             11577..14708
                     /locus_tag="P5558_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY23340.1"
                     /translation="MARFFIDRPVFAWVIAILVMGVGILAVLTLPIAQYPQIAPPSVN
                     VTATYPGASAETVENTVTQVIEQQMTGLDGLRYIKSSSSSAGRAEIALTFTTGTDPDI
                     AQVQVQNRLAQATPLLPETVQRQGIEVRKSSAGFLMVIGLIAEDGSLEQVDLSDYLSS
                     NVVDEISRVPGVGAVQVFGAPYAMRIWLDPAKLAAYELAPGDVVAAVSRENAQISAGA
                     LGELPTVEGQQLNATVTAQSLLQTPGDFEQIVLRAETDGGLVLLKDVARVEIGAQSYS
                     TIGRYNGQPASGMAIQLASGANALETADRVRARMDELAAFFPEGVVYNVPYDTTPFIS
                     ISIEEVVITLFEAIVLVFLVMFLFLQNWRATLIPTLAVPVVILGTFGVLAAFGFSINT
                     LTMLAMVLAIGLLVDDAIVVVENVERIMEEEGLDPRAATRKSMGQITGALIGIAVVLS
                     AVFVPMAFFGGSTGVIYKQFSITIVSAMFLSVVVALVLTPALCATILESRTSHRKQRG
                     PIGWFNRFFDGTQRGYASSVGWIVRRPVRVGLVYLLLVAGMIGLFARTPTGFLPDEDQ
                     GIMFTFVQGPAGATATRTLDVLDQVESHFLENEAENVDSMFTVVGFSFAGSGQNVGLG
                     FVRLKDWDERPRADQDVHAIAGRAFGAMMAIQDAMVFPIVPPAVIELGNVSGFDFYVQ
                     ARAGQSHQQLLEARNQLIGMASKSPLIGSIRPNGLEDAPQYGLDIDWRKAGAMGVTAN
                     DVSDLLTVAWAGRYVNDFLDRGRIKRVYVQGEADARSTPEDIERWRVRNASGGLVPFS
                     NFIEGGWRFGPQGLYRYNGVPAMQLQGSPAPGVSTGEAMAEIERLVAELPPGFDISWT
                     GLSLEEQESGNQAPLLYFLSVAVVFLSLAALYESWVVPAAVMFAMPIGVLGALVGAWI
                     GGFENGVFFQVGLLTTIGLTGKNAILIVEFARARLEEGEELLTAVIEAARQRFRPIIM
                     TSMAFSLGVLPLVLSSGAGSAGRRAIGSGVLGGTITGTVLGILFVPLFFVLIRRLVPR
                     REAAKAAAPLPAA"
     gene            14796..15776
                     /locus_tag="P5558_03155"
     CDS             14796..15776
                     /locus_tag="P5558_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY23341.1"
                     /translation="MGETQVIKKRRLGRTGLEVTPICFGTSGLGDMPSTYGYSVDEER
                     GRATVRAILAGPVNFLDTSRNYAMGRSEQRIGAVIRELGGLPEGFVVSTKLDRDMETR
                     EFDAARARRSLEESLKALHLDRVQLLHLHDPEHAADLGPITRKGGAIAELFRMKEEGL
                     CDAVGLAAGRIDIMMPMLKDWEFDALITHNRFTLTNRNALPMLELAKERGIAILNAAP
                     YAGGVLAKGTDSFKRYVYQEASAEVLDPVRAIEAVCARHGVPPGAAALQFSMRDPRVS
                     STICGVTRPERVTQTLEWAAFPIPDAAWDELLALPFATDDPEATRVYDPG"
     gene            complement(15789..16190)
                     /locus_tag="P5558_03160"
     CDS             complement(15789..16190)
                     /locus_tag="P5558_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRY23342.1"
                     /translation="MTAEAFFDTNLVVYAFIDSGDRSEVAERLLANGGIVSIQVLNEF
                     ISVARRKRASTWTDIRTALATLGTLCGEPRPLTATTHDAALNIAERYGYHIYDALIIA
                     SAAEAGCRTLYTEDMANGQVIEGVRLTNPFA"
     gene            complement(16180..16446)
                     /locus_tag="P5558_03165"
     CDS             complement(16180..16446)
                     /locus_tag="P5558_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009503866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY23343.1"
                     /translation="MRVARWGNSLALRLPARVVRELALVEGDEVEIRVATSRSVEIAR
                     DEQREKALETMRQLRRRSAPLPPDYRFDREEAHERGSRDLPNDR"
     gene            complement(16513..17496)
                     /locus_tag="P5558_03170"
     CDS             complement(16513..17496)
                     /locus_tag="P5558_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23344.1"
                     /translation="MRILITGAGGFIGRKLTERLLADGTVDGRTVQHITAADLYGLDL
                     LADDARLARVALDITDHVAAAKLIAQGYDLVVHLAAIVSANAEEDFDLGYKVNLDGTR
                     YLLEGLKALGTCPRLVFASTLAVFGGPVPTPPALIGDDFHLTPQTSYGAQKAACELLV
                     ADYTRKGYIDGRSLRLPTIVVRPGKPNKAASTFASSIIREPLAGQAAICPVDPDSTMY
                     ILSPRRVIDAFVKTAGLPSDAFGQVRSLTLPGIAVRIGDALEALRRIAGDKVADRVKQ
                     VPDPKIQKIVDGWPYNFASKRARDMGYEADASIDEIIQAHIEDEHGGRIED"
     gene            17638..18972
                     /locus_tag="P5558_03175"
     CDS             17638..18972
                     /locus_tag="P5558_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY23345.1"
                     /translation="MTFRFTRRDSIKLGAGVGAAALLGSTALRAEIPTYDATEPDLPI
                     EDGAALRVLRPSKFVAGDEELFNQNSQAFTAATGVDVRIDYEGWEDLRPKLAVAANVG
                     QGPDIALAWSDDPHKFVDSCLDLTDVAEYLGEKYGGWYPLAERYGTNGGRWIAMPMGA
                     SGGRVVYRKSIINEAGYDAVPGDVDGFLQMNQKLAAMGKPGGLALGNAVGDGNAWCNW
                     LVWAFGGAMVDENDQVIINSPETIEALKYGKALYDTFIPGTLSWLDPSNNKAYLAGEL
                     SWTQNGISIYYAAKNSEDAAVKAIAEDTYHSRMPIGPVGMTTERCLMVNAMVLNYTPY
                     PNAAKRYLQFMMEHEQYDQWLTAAIGYWGHPLKAYEASEIWSVDPKHEAFKSVIADSL
                     WDGYKGSIGEASAAVLSDFVVVNMVASVCAGQATPEEAAAEAERRAKRYYRG"
     gene            19049..20002
                     /locus_tag="P5558_03180"
     CDS             19049..20002
                     /locus_tag="P5558_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY23346.1"
                     /translation="MAATSDTYVAQTRPDNWLARLFDNKAFLIVVCMTPAVGLLVLFL
                     TYPLGLGVWLAFTDTRIGRAGEFVGFFNYELLFSDRVFWMAVWWTVFYTVFATIFKFA
                     LGLWLALLLNHYVPFKSVVRAIILVPWIVPTVLSAIAFWWIYDPQFSIISYVLVDVLG
                     WRENYIDFLGDPWNARWSLIAANVWRGIPFVAICLLAGLQTISPSLYEAAMLDGATPW
                     QRFVHVTVPMLMPILAVVLTFSVIFTFTDFQLVYAITRGGPVNATHLMATLAFQRAIP
                     GGQLGEGAAIAVAMIPFLIAATFFSYFALARRKWQQGGDDD"
     gene            19995..20903
                     /locus_tag="P5558_03185"
     CDS             19995..20903
                     /locus_tag="P5558_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY23347.1"
                     /translation="MTEAARAIDQPPAVNTGERQGAISWESLPRRIVTIYTPLVIFMI
                     VLLFPFYWMTITTFKSNEELYNYKDYNPFWVSSPTLANINKLLFETDYPTWLMTTMTV
                     AVVSTILSVFASVLAAYAIQRLRFRGSQWVGLSIYLAYLVPPSILFIPLATMVFQFGL
                     FDSQLALILTYPTFLIPFCTWLLIGYFKSIPYELEECALIDGASRLQILIRITLPLAV
                     PGLISAGIFAFTLSWNEFIYALAFIQSAENKTVPVAVLTQLVEGDVYHWGSLMAGALL
                     GSVPVAIFYSFFVEYYVSSLTGAVKE"
     gene            21014..22399
                     /locus_tag="P5558_03190"
     CDS             21014..22399
                     /locus_tag="P5558_03190"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HRY23348.1"
                     /translation="MGRKRLVVCCDGTWNEPVTRTNIWRLKNAIASREVLADRAEIPQ
                     WVYYDAGVGTERGNRLTGGMLGMGLSRNLKEAYRFLAESYAEGDEIWCFGFSRGAYTA
                     RSLCGFLGVAGLLRHRDLGLLDDAFEYYRLPKAKREASRFGRIRDDLHLHTKEKCLSV
                     RLLGVFDTVGALGVPLPWLKALTQWRSVQFHDTELSSLIENAYQALAIDERRGPFQPT
                     LWTGAPGEARSCDGRMVPQRVVQAWFPGVHSDVGGGYDEKAYADVTLDWMLDVARSLG
                     LVLREDYVERQLRPDHLASLHESYTTGWRLADRIPGFTRGGARPIGNEQRRARGRGPI
                     APTEVLHWTASARAAAAGARLPSGRSPYWPLNLCDGEGALHPTLAGLRHEKRRAARRL
                     LDEVCTLDGRAAELVDLSRAGARVRLAAGTPPPSGAGALRLVSRTTGARLGSVVWRRE
                     GLIGLVFEDAA"
     gene            complement(22406..22750)
                     /locus_tag="P5558_03195"
     CDS             complement(22406..22750)
                     /locus_tag="P5558_03195"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRY23349.1"
                     /translation="MIAVIFELWPKPEQRDAYFELAAELRPLLEQIDGFISVERFESV
                     SEPGKFVSLSFWRDEAAVAAWRSLAEHRLAQARGRGQIFADYRLRVAEVSRDYGLTDR
                     AEAPADSRARHG"
     gene            complement(22747..24849)
                     /gene="glgX"
                     /locus_tag="P5558_03200"
     CDS             complement(22747..24849)
                     /gene="glgX"
                     /locus_tag="P5558_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRY23350.1"
                     /translation="MNRLATNPGRPEATSPARRLCPGHPWPLGATATEGGVNFALFSA
                     HATTVELCLFDPTGRIEQARLTLPEWTDEVWHGFLPDAGPGLVYGYRAHGPDDPAAGH
                     RFDPAKLLLDPYARHAVGAFDRAALRPGGAACRFGAETAPHVAKAVVAGPDQHVPGAW
                     SRPRNGRPETLIYEAHVKGLTRLHEGVPADRRGTYAALADPRVVEHLQRLGVTALELL
                     PVQAFVDEPHLIEKGLTNYWGYNPINWFMVEPRYAATADPLGELREAVAALHAAGIEV
                     LLDVVYNHTGEGDGQGPCLAFRGLDNASYYRLDAEAADGYANLTGCGNTVDASHPRVL
                     QLIMDSLRYWVEVVGIDGFRFDLAATLGREPLGFDKGAGFFDCLKQDPVLAHVRMIAE
                     PWDIGPEGYRMGGFGPGWSEWNDRYRDVVRGFWRGDEGLLPELAARLLGSADLFEHQG
                     RRPWSSINFVAAHDGFTLADLVAHEMKHNEANLEANGDGHGHNLSWNNGVEGPSDDPA
                     VLAARGRDVRNLLATLFLGQGTVMLAMGDELGRTQQGNNNAYCQDNALSWLDWATADT
                     DLIAFTAELAAIRRAHPVLRRRHFLHGSVAAADGTPDVQWLKPDGTALEPDDWHDPAA
                     RAILLALAGDAPTEIDDDGHPSLDARFVLALNAGRVALELHLPPGDWSVRLDTSGEAS
                     TRSLPARSVVLYQQEARR"
     gene            25024..26940
                     /locus_tag="P5558_03205"
     CDS             25024..26940
                     /locus_tag="P5558_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylosucrase"
                     /protein_id="HRY23351.1"
                     /translation="MEGDPARALDELRRRLAPLLEQAGPGRPALEARLEAVFEQLFAA
                     LSALYGHRWDFAWQLEAVLRTALEAALARPERLAARDRERRPDWIRESRQVGAMGYVD
                     RFAGSIVGLFDRLPHLEHLGVTYLHLMPLFAVPDGPNDGGYAVSSYREVAPRLGSMAQ
                     LADLAAALHDRGMALVLDFVLNHTADDHAWAEAAKAGDPAMQAFYWMFDERGEVEPFA
                     RSLRQIFPDRGGDAFVWRDDVAGGKWVWSTFYPFQWDLNYTNPEVLRAILGEMLFLAN
                     QGVDVLRLDAVPFLWKKAGTPCENLPEAHLVVRAFNAAAAIAAPSLVFKSEAIVHPDE
                     VARYVRVDECRLSYNPLLMCSLWDALATRDTRHLRDVLARRLRTAEGTAWVNYLRCHD
                     DIGWGMADEDMTELGIAPEDHRRFLNAFYTGRFPGSFARGLPFQENPRTGDCRVSGTC
                     AALAGLEQAEELGDATLLEHAVRRIVLLHGLVYTMGGIPLLYLGDEIAQPNDPSYTSR
                     PAEAADNRWVHRPFFDDARLQRALAEPATPAGRVLEALVRLKELRRSEPALAVGPVEL
                     LELDPRYTLAYRKRAQGRSLTVVANLCEHEVDIEAGGLGPGPFLDLWGGRNLEPARLR
                     SLEAYGLLVLRRDG"
     gene            complement(26986..27975)
                     /gene="msrP"
                     /locus_tag="P5558_03210"
     CDS             complement(26986..27975)
                     /gene="msrP"
                     /locus_tag="P5558_03210"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HRY23352.1"
                     /translation="MLLPSRRPRWKLAESLVTDERLAFGRRRLIGGMAAAAGLAVAGC
                     KEEASQAVAATEAAAPADPSAALYPAARNAAFDLPEPWTPERLAAGYNNFIEFGSHKN
                     IQRAAQALPTRPWTVTVDGLVAEPMTIDIDDLLRRMPLEERVYRHRCVETWAMIVPWS
                     GFALRHLLDLAEPLASARYVLFQTFEDSAVAPGQRAFWYPWPYTEAVTLAEAGNDLAF
                     MVTGLYGKPLPRQNGAPLRLALPWKYGYKSIKSIERISLVEERPDTFWNVVDGNEYGF
                     WANVNPAVPHPRWSQATERLLGTQEDVPTLLFNGYAEEVAGLYPNLMDKSLYR"
     gene            28342..28650
                     /locus_tag="P5558_03215"
     CDS             28342..28650
                     /locus_tag="P5558_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23353.1"
                     /translation="MRFLGALSLGLVGPVVVAPLAVALPIAGTAPSARPEGAPTIEQF
                     DPPEGWMERALRGISEPIPGGLEFLQDQGAWFTPFNHPNATGRYDIRGLHGSEGTPSG
                     "
     gene            28812..>29075
                     /locus_tag="P5558_03220"
     CDS             28812..>29075
                     /locus_tag="P5558_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23354.1"
                     /translation="MSSKKERNLTLAALCTAALLALPVATSAQGMGGMSGMGGMGGMS
                     GMGGMGGMSGMGGNSIGQWVCTQWTSTGMGGMSGMGGMSGMGGM"
BASE COUNT         4263 a         9787 c        10347 g         4578 t
ORIGIN      
        1 cgggccacgg tgctggtcga cgagctgatc gtcgcggtgg ccatcaatgc cggcaaggag
       61 ccgctcttca gcctcgagga gcggacggcc atggtcgagg cggacatcaa cgccatgatg
      121 gccgccaacg agcacaatgg tgcccgggtg accgtcctgc cgttcaagaa cctcctgatg
      181 gccttcgccg ccgacaacga cgccgacttc atcattcgcg gcctcagggc ggtctccgac
      241 ttcgagtacg agttccagat ggccgccaac aacaagcggc tctaccccaa gatcgagacc
      301 gtcttcctga tggcgtcgga gaccaaccag ttcatctcct cgcgtttcgt caaggagatc
      361 catctgctcg gtggcgacgt caccagcttc gtctccgagg cggtgatggt gcagttgcgc
      421 acgaagagcc gggactgact tggacgtcga tgcgttcgac ttcgagctgc cggcggatct
      481 gatcgcgcaa agaccggtcg agccgcggga cgctgcacgg ttgctggtca tcggcgacaa
      541 ccttcaggac cgcacggtgc acgacctgcc cggcctgctg gcgccgggcg atcttctggt
      601 gctcaatcgc acgcgcgtgc tgccaacgcg gttcgccgcc aggcggcaac gggggggccg
      661 gccgatccag atcaccctgg tggccgacca gggtgacgac cggtggtcgg ccttcgccag
      721 gcccggccgg catctgacac caggcgacac gctggagctg gcatctgggc tcgtggcgga
      781 ggtcttggcc aaggatgccg acgggcgctt cgccctgcag ctccaggccg aagacgggac
      841 ggtgatcgat gccatccggg ccggcgggtc gatgccgctg ccaccctata tccgccgccc
      901 ggccgcgggc gaggcagccg accgcgaacg ctaccaggcg attttcgccc gggacgaggg
      961 ctcggtggcg gcacccacgg cgtcgctgca tttcaccgac cgcctgctcg ccgacctcgc
     1021 ggcacggcgt gtcggccacg ccttcctgac cctgcatgtc gggctcggca cgtttctgcc
     1081 ggtgaaggtg gcaaaggcgc gggatcacgt catgcatccg gagcgctacg tgctgcccga
     1141 ggcgacggtc gatgcgatcg ccgccacgcg tgccgccggc gggcggatcg tcgcggtcgg
     1201 cacgaccgtg ctgcgggtgc tcgaggcctg cgcgcgggaa accggggagc tccatcccgg
     1261 gcagggcagc acggcgctct tcatcacgcc gggcttcgac tttcgggtgg tcgaccgcct
     1321 gctgaccaat ttccatctgc cgcgatcgac gctcttcatg ctggtcgcgg cactggcggg
     1381 gctcgaccgc atgcaggcgg cctatgccca tgcgatcgcc agccgctatc gcttcttcag
     1441 ctatggcgat gcgtgcctga tcgaccgccg ggactgacgc cgcggcctct caggcgctgc
     1501 gtttgcgccc gtccagccgc tccgccagcc ggtcgaggaa atggccgacc gccgccacgt
     1561 cggccaggcg gtagtgggcc gccgtcgact gcggcccgtc ctggccgacg gcgatgccga
     1621 taccccagcc ctcgatcgcc cgaaaggcat cctcgtcggt gacgtcgtcg ccgatataga
     1681 ccggcagggt ccgctcgcgc gtccggccgg cgctgtcgag gatccaggaa agcgccttgc
     1741 ccttgtccca atccaaggcg ggcacgaact cgaagacctt cttgccggcc ttgaccgtca
     1801 gctcggggaa cgcggctgcc gtgtcgcgaa tggctcgttc cacatcaccg gtggcggcat
     1861 cgccgacgcg gcgatagtgc accgcgaggc cgaagcgctt gcgctccagc tgcacgccgg
     1921 cgatggggcc gagccgctgg gccagcgcct tgcccagctc gcccagccgg gtcgctgccg
     1981 cttgcgcttc gtcgtgctcc tccagccggc cgtcggggtg gcgaagctcg aagccgtggc
     2041 tgccagcgaa gaagaggttg cgcagcccga cccgctcgac cagatcgccg agaccccggc
     2101 cgctgatcac ggcgacgaaa tgccggcccg cgagctggcg cacgcgctgc cgcatcgtct
     2161 cgtcgaggat cgcatcctct ggccgctcga cgatgggtgt cagcgtgccg tcgtaatcga
     2221 gaaagagatg caggacgcga tcgcccgcca gcgccacgat ggcgtcgatg gaggccaggg
     2281 cgtcgggcag ggcggcacgg tcgcgcaacg ggcggtctcc cttgatatgc agatggtcga
     2341 gcgaggcgac gacgctcgcg gcgcccgcgg cggcgaggcc tgcagtctcg ccgtcgcgag
     2401 cgacgccgac aaccaggccg aagcccccgc gacatcccgc ctctatcccc gcgacggcgt
     2461 cctcgacgat cgctgcccgt gatgccggca cgccgagcgc ctcgaccgcg gccaggaata
     2521 cgtcgggtgc gggtttgccg gcgagacctt ccgccgtggc gacggttccg tcgaccagca
     2581 cctggaagag cgggcgaatg cccgcggccg tcagcaccgc ctcggcgttg cggctcgccg
     2641 agatcgccgc gaccttcagg tcggcgcggc gcgcgcggtc gacgaaggca accgcatcgg
     2701 cgaaggcgct gacaccctcg cgggcgacgc gatcgaggaa ggcctggttc ttgcgtcgac
     2761 cgagagcccg gatcgtcggg gcatccggtg cgtcgtcgct gccgccgaga ggaagatcga
     2821 taccccgcga ggcgaggaag tcacgtacgc cgtcgagccg gggcttgccg tcgacgtgcc
     2881 ggcgatagtc ggcggtcgtg aaaggggcga agcccctgcc gtcacgggcc tcgagaaacg
     2941 cgtcgaagac cgtcttccag gccgcctcgt gggtgcgggc ggtgtcggtg agcacgccgt
     3001 ccatgtcgag gatgacggcg tcgatgcgcg atggatcgag cacgggcgct gcgtcgctca
     3061 tttggccgtg gcgcttttct gccgggatgc ctcggcctcc gccacgatcc gcttgaccgc
     3121 catgaagctg tcggggctga ccgagatcga atcgatgccg tgctcgacca ggaaggcggc
     3181 gaactcggga tgatcgctcg gcgcctggcc gcagatgccg gtcttggtgc cggccgcgcg
     3241 cgccttggcg aggagcgtgg cgatcgaggc ctcgacggcc gggtcgcgct cgtcgaagag
     3301 gctgctcagg atcgagctgt cgcgatcgac gccgagcgtg agctgggtga ggtcgttcga
     3361 gccgatcgag aagccgtcga agcgctcggc gaacttctcg gctaggatga tgttggcggg
     3421 gatctcgcac atcatgaaga cctcgagccc cgcccggcca cgcatcaggc catgctcggc
     3481 catctcggcg agcacggcgt cggcctcgcc gaccgtgcgc acgaacggga tcatcatgac
     3541 gatgttggtg aagcccagct cctcgcgggc ccgccggatc gcccggcact cgagcgcgaa
     3601 gccttcgcgg tagccgtcgc tgtagtaccg cgacgcaccg cgccagccga gcatcggatt
     3661 ctcctccttc ggctcgaagg cgcggccgcc gatcagctcg gcatattcgt tggtcttgaa
     3721 atcgctcatc cggacgatca ccggggccgg gtgatgcgcc gccgcgatcc gcgcgatgcc
     3781 gcgcgccagc cggtcgacga agaactcggg cttgctcgcg tagccgatcg tcagctcgtc
     3841 gatctggcgg cgggcgtcct cgtccgtcag ctcgtcgaac ttcaccagcg ccatggggtg
     3901 gatgcggatg aggttctcga cgatgaactc catccgggcg aggccgacgc cgtcggtcgg
     3961 cagccgccac caggtgaagg cacccgccgg gtcggcgagg ttgagcatga ccttggtcgt
     4021 ggtggccggg atgctgtcga tcgcgatctc ctcgacctcg aagtccagga gccccgcgta
     4081 gacgaaaccg gtatcgccct cggcgcagga cacggtcacc tcctggccgt cggccagctt
     4141 cgccgtggcg tcgccggcgc cgaccaccgc ggtcaggccc agctcacggc tgacgatggc
     4201 ggcatggctg gtgcggccgc catgatcggt cacaatcgcg gccgccttct tcatgatcgg
     4261 cacccagtcg gggtcggtca tgccggtcac cagcacgccg ccctcgacaa agcggtcgat
     4321 ctcggtgggg ttggccagct tgcagaccgg tccgctgccg atcgaggcgc cgatcgagag
     4381 gcccttcacc aagggctcgc cctggccggt caggtgatag gccttgagca tgccggcagc
     4441 ccgcctcgac tgcacggtct cggggcgagc ctggaccacg aagagctcgc cggtgagccc
     4501 gtccttggcc cattcgatat ccatcggccg gccgtaatgc cgctcgatgc ggaccgccca
     4561 gcgggcgagc tcgaggatgt cgtcgtcctc gagcacgaag cggtgctgct cggccctggc
     4621 ggtcgagacc gtcctgaccg cctccttgcc ggtggtgaag accatcttgc ggtccttcgc
     4681 cccgagccgc ttctcgatga tcggtcggag cgcggtgtcc tccaggagcg gcttgaagac
     4741 ggtgtactcg tccgggtcga cgatgccctg cacgaccagc tcgccgaggc cccaggcggc
     4801 atcgatcagc acgttgcgcg ggaagccggt ctcggtgtcg atcgagaaca tcacgccgga
     4861 gccggccttg tcggagcgga ccatgcgctg gatgccgacc gagagggcca ccttcaggtg
     4921 gtcgaagcgg ttgttgacgc ggtaggcgat ggcgcggtcg gtgaagagcg aggcgtagca
     4981 gcggcgcagg gcacggagga gcgcggcctc gccctggaca ttgaggtagc tttcgagctg
     5041 gccggcgaag ctcgcctcgg gcaggtcctc ggcggtggcg ctgctgcgga ccgccacgct
     5101 gaccggctcg ccgccctcgg ccatcgcggc ataggcccgg cggaccgcct cggcgagcgc
     5161 cggcggcacc tcggcgttct ccagaagctc gcgcacggcg cggccgacac gggcgagatt
     5221 gctgtggtcg tccttcagct tcgccatcgt ttcggcgatc gcgtcgcgca ggttgttcgc
     5281 gtcgacatag tgccagtagg catgggcggt cgtggcgaag ccgcccggca cgcggacccc
     5341 ctgggcctgc agattggcga tcatctcgcc gagcgaggcg ttcttgccgc cgacgcggtc
     5401 gacgtcggtg cggcgcagct cgccgagctc gatcaccagc aggtcttcag tcggcatcgg
     5461 tcgtctcccc tcgcacgttg ccgcgcttgc gctccgatgg gcggatcaga cggaactcgt
     5521 ggctgtcgcc cgggctcaat ttgcggaaat gactacggta cgcgatctcg atcatcggcg
     5581 ccaatgccgg ccggctgcgc acgcgcagcc ggctccgggt cgcctcgacc tcgaggtgat
     5641 gtcggcgata gcgcaggcag acgcgcaggc aatccacgcc gacgggcaga agcgggttga
     5701 aatggagcac gccggagcgg gtctcgagcc cgagatagtt gcgctggacc agatcgaccg
     5761 tgccggccat ggcaccggta tggatgcctt ccggcgtggt gccgccctgg atgtccagga
     5821 tatcgctgtc cagcgcccgc ctgaagaggt gccaggactg ctcgcgatcg gcgcgggcca
     5881 gcacccaggc atggacgatc aggctgagcg tcgagccatg cgaggttcgc gcgagatagt
     5941 agtcgatgtt gcgccggatg gcagcggagt cgaacgggta gcccaggccc tcgaagatca
     6001 ggtccagctc ctccgccgag aagaggtaga agagcatcag gacgtcggcc tgcttcgcca
     6061 ccttgaagtc gttcgggctc ttgccctcgg cctcgaggat ccggtcgagc ctttggatat
     6121 tgccgtattt ctcgcgatag gcgggccagt cgagctcgac gagctgctcg tagccgtcga
     6181 actggctgat gatgccgtcg ccatggaacg gcaccttcag cttgcggctg acatcgtacc
     6241 agagctcgag atcggcgcct gcgatctcga gccgggcgaa gagctcgtcg gtgcagtcgt
     6301 gtggcaggac cgccaccatg tcgagggcgc gggtcaggag ccaggccacc atgacgttgg
     6361 tgtaggcatt gttgtcgatg ccgccctggg cttccgggtc ggtgcccggg taggcggtat
     6421 ggaactcgtc cgggcccatc acgcccttga tgtcgtagcg gtcgatcgcc gcgtcatagg
     6481 tggcggccga tacccagaac tgggcgatcg cgaagaacat ctccgcaccg taatgccgga
     6541 tgaagccggt atcgcccgtg gcttcccagt agcgccagat attgtagcag atggcggcgt
     6601 tcacgtggcg ctggcggtgg ctcgtgtcgg gcagccagcg gccggattcg gggttgaggt
     6661 ggagcttttg ggtctcctcc cggccgttcg agccgctctg ccaggggaac atggcgccct
     6721 tgaagccctc ggcccgggcg gcgaggcgcg cctggcccag ccggcggtag cggtagcgga
     6781 tggtctcgcg cacgaggtcg ggcacgcgca gcaccagaaa gggcaggatg aagagctcgt
     6841 cccagaagat gtggccgcga taggcctcgc cgtgccagcc gcgggccggc acgccgacat
     6901 cgtagtcgac cgagttggcg gagatggtct gcagcaggtg gaagatgtgg acccggagat
     6961 gggtcgcctc gccgtcgccc tcgggacggt cgaaggtgat ctcgcagctc ccccagagct
     7021 gctcccaggc cagcagatgg cgggccagca gcgcctcgaa gccggtgctg cgggtgaggc
     7081 tttcggtcgc ctcgagcccg gcctcggcga tcgccggatc ccggctggtg tagagggcga
     7141 cgagcttctc gatccgcaag gggcggtggt cgagctgcac gcgcgccaat tgctcgatct
     7201 ggtcggacgc ctgcaccacc tcgatcggcc ggtcgagccg ttcgccgtca cgaaagagac
     7261 cgaggcgcgc cgcctcgacc acctcgatcc gcgactgcgc ggtgcggcag cggagcagca
     7321 tgccgtcgct gccgaaggct tccgtcccga gcgtctccag atggcggttg gccagcgcct
     7381 tgtagcgctc gaccccggca ttgacgacgc tgccgtcgat gcccgagcgg atggtgatct
     7441 cgccggacca gtcctccgcc accagctcga cctcgagcgc tgcctcgtgg cggtcggcca
     7501 tgctgaccag ccgccgctcg cgccagcgcg tcgtccggcc cgcctgatcg cggaagcgga
     7561 agtcgcggtg gagcaggccc tggcgggtat cgagctgctg gacgaaatcg agaacctgca
     7621 cctcgtcgat ggcgaaccag gggccgtccc cgatgcggaa ggtcaaaggc agccagttcg
     7681 gcacattgac caggtcctcg ttctcgacgt cgcggtcgcc gatccggctg gtcaggcggt
     7741 tgtagagccc cgccacatag gtgccggggt agtggacagc gtcggcgccc gtgcaggtga
     7801 cggcgccgcg cgtgcagaaa tagccgttgc ccagggtcgt gagcgattcg cggagcttct
     7861 cgcggtccgg ctcgtaagcg tcgtaggtga gcgaccagct catgcatgca cctttgctgt
     7921 cgggcggggc cgaccttggc cagcaatcgc ttgaccgtag cacgcaccgc cacgactgga
     7981 aggggcgcat ttcgccgccg cgcttggcct ttgcggcggc ttctcctaga gaagtcgccc
     8041 gaacgacgtc gccgggcgag gcggcgcgca gcagcgaggc agcagcgatg gccggagcgg
     8101 aggcccttct cgagcggatc agggacgaag gcgtgcgcag cctcgatctg cgcttcaccg
     8161 acctcgccgg ccgctggcgg cacaccacgc tggacgcggc gggcgtcaag ccgttccagc
     8221 tcgagcacgg ggtcctgatc gacggctcgg ccgtgcctgg ctggcgcgac gtcagcgaat
     8281 ccgaccttct gctgcggccc gacctcgcct cggccttcca ggaccccttc gccgcccagc
     8341 ccacgctctg cctcttcgcc gacggggcgg agccggccac cggcatgagc tacgagcgcg
     8401 acccgcgctc gaccgtggcg cgggccgagg cccatctcct ggagagcggc atcgccgaca
     8461 cgctggtcgc ggcggccgag atcggcttct tcatcttcga cgacgtgcgc atcgagcggg
     8521 ggccgctcaa ggccggctat gcgctggaat cgagcgaggc ccggacggcc ggcggggccg
     8581 cctatgcggg gggcaatccg ggccaccggc cggcgccggg tgccgcccag gcctgcctgc
     8641 cgccggccga ccaccatgcc gacatccggg ccgagatcac gaccacgctg ggcggcctcg
     8701 gcttcaccga gctgcgccac gagcacggcc cggcggcggc ccagcaccgg ctgatgatcg
     8761 gcaagggggg gctgctcgcc accgccgacc ggctgcagct cctgaaatac gtcgcccacc
     8821 aggtgaccgc cacctatggc aagtcggcga gcttcatggc caagcccatc gccgacgagc
     8881 cgggggccgc cctcttcctc gcccagtcct tctggcaggg cggcaagccg ctcttcgccg
     8941 gccagggcta tgccgacctg agcccgctct gcctgcagtt caccgccggc atcctggcgc
     9001 acggccgggc cctcaacgcc ttcaccaacc cgaccaccaa cagctaccgc cggctgcagc
     9061 cgaacctcga cgagccgacg cttttgacct tcgccgcgca caaccgttcg gccgcgatcc
     9121 gcatccccta tgccgaccgg ccggaccgga agcgggtcga gctgcgcttc gccgacgcct
     9181 cggccaaccc ctacctggcg ctcaccgcca tcctcatggc ggggctcgac ggcatcgccc
     9241 gcaagctcga gccgggcgac gccatggaca ggaacctcta cgacctccgg gccgaggagc
     9301 tggagggcat cccctccacc tgccggacgc tcgaggcggc gctcgacgcg ctcgacgccg
     9361 accacgagtt ctgcctgcgc ggcgaggtga tgagcggcga gctcatcgac gcctacatcg
     9421 ccgtcaagcg ggccgaggtc gagcacctgg cccggctgcc gaccccggcc gagttcgagc
     9481 tctattacgg gctctgataa aggaataata gcctatttag ggctgccggt caaggcccgt
     9541 gggttccatc ggcaaaatcg gctttccggc cgtcgacagc ctgccgcaaa ccgggcgaat
     9601 acgcagaaaa ccgcgttccc tcaacgggtt ggagtgcgtc gggttcgttg gtcgaagctc
     9661 gggttcattc gtcgaatcgc cgggttcgct cgtcgatttc gtgggttcgc tcgtcgattc
     9721 ggcaggttcg ttcgtcggca gatgtcgcgg attccgcgac atttccggaa tcctgtcgca
     9781 ggaaaagcga caattgccgg ccccatgtcg cagattccgc gacaacccgg gcaaagttgt
     9841 cgcggattcc gcgacaaccc gagggtcgct gtcgcggatt ccgcgacatt ggggagcgtc
     9901 agggccgtgc cgggatggcg aagccggcga ggaagacgcg gatggcgagg tcgagccggg
     9961 cgcggcgctg cccgagcgcg ggcggcggtc ggcccgggtc caggagctgc tggagcgggc
    10021 aggcacaggc cgtgtcgcag agcagcctgg cggcggcccc gagatcgtcg caggcgcgct
    10081 cgccccgcgc tgcagagctg ggcgcttgcc atcacgacgg ggctccctac tttggagacc
    10141 gcgcggtttc ctcggggcgg gtcgatcccg catgtcacat tccgcgctgg aatgccaagg
    10201 agtcacgcaa tgaggcgatc ggttccggtg cccggggcgg ggctggcggg cgccctgctc
    10261 ggcgccgtcg tcatgctggc tgcgatgctc ggcagcgggg tcggggccgc ctgggcgcaa
    10321 gcggcgccga ccccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgcga
    10441 tgctcggcag cggggtcggg gccgcctggg cgcaagcggc gccgaccccg gtgacggtcg
    10501 tgacgctgaa ggcgcaggat ctcacgctga ccagcacgct gccgggccgc gtggtcgcct
    10561 ccggcgtggc cgaggtgcgg ccgcaggtcg acgggatcat cgtcgagcgg ctctacgagg
    10621 aaggggcgca cgtcaaggtg ggcgatctgc tctaccggat cgattccgcc acctacgagg
    10681 cgcaggtcgc ggccgccgag gcccaggtgg cccaggccca ggcccggctg acctcggccg
    10741 agaaggatgc cgaccgggtg cgcgccctgg tcgaccggcg ggcgacgagc cagcaggcgc
    10801 tcgacgacgc ggtggccgag cgcgacgccg ccgatgccgg gctgaaggtg gcccaggccg
    10861 agctcctcgc ggcgcagatc gacctcgacc ggaccaccat cagggcaccg ctctcgggca
    10921 tcatcgggcg ctcgctgacg acccagggct cgctcgtcac ggccgggcag gccgcaccgc
    10981 tcgccatcat ccgcaccatc gacccggtgc tggtcgacgt cacccagtcg gccgccgaga
    11041 tcatcgcctg gcggcgcggc gccaccgcgg aacgcctcgc cgacgcgcag cagacggtga
    11101 cgctgaccct ggccgacggc aagccctacg aggagaccgg ccttttgacc gcggccgagc
    11161 cctatgtcca cgagcagacg ggcgtggtga cgctcaggct ggagttcccc aacccgaccg
    11221 agctcctgct gcccggcatg tatgtgcagg tggagttgcc gcaggcggtg gccaggaacg
    11281 tcatcctggc cccgcaggag ggcgtgatgc gcgaccatcg cggccggccg atcgcctacg
    11341 tggtcaatgg cgacgacgtg gtcgaggagc gggtgctggc cgtgctgcag gcgcatggcg
    11401 ccttctgggt caccgccgac ggcctcgccg atggcgaccg catcgtcgtc gcgggcctgc
    11461 agaagatccg gcccggcgcc accgtcgccc ccgccgagcg cgaggcggca cccgacgcca
    11521 cttccgccaa cctcgccgac agcaacggcg gctaggagca aggcagggca gcccgcatgg
    11581 cccggttctt tatcgaccgc ccggtcttcg cctgggtgat cgccatcctc gtcatgggcg
    11641 tcggcatcct cgcggtgctg accctgccga tcgcccagta cccgcagatc gccccgccct
    11701 cggtcaacgt caccgccacc tatcccgggg cctcggccga gaccgtcgag aacacggtga
    11761 cccaggtgat cgagcagcag atgaccgggc tcgacgggct gcgctacatc aagtcgagct
    11821 cgtcctcggc gggccgggcc gagatcgcgc tcaccttcac caccggcacc gaccccgaca
    11881 tcgcccaggt ccaggtgcag aaccgcctcg cgcaggcgac gccgctcctg ccggagaccg
    11941 tgcagcgcca gggcatcgag gtgcgcaagt cctcggccgg cttcctcatg gtgatcggcc
    12001 tgatcgccga ggacggctcg ctcgagcagg tcgacctctc cgactatctc tcctcgaacg
    12061 tggtcgacga gatcagccgc gtgccggggg tcggcgcggt gcaggtgttc ggcgccccct
    12121 atgcgatgcg catctggctc gaccccgcga agctggcggc ctacgagctg gcgccgggcg
    12181 acgtggtggc cgccgtctcg cgcgagaacg cgcagatctc cgcgggcgcc ctgggcgagc
    12241 tgccgaccgt cgaaggccag cagctcaacg ccacggtgac cgcccagtcg ctgctccaga
    12301 cgcccgggga tttcgagcag atcgtgctcc gggcggagac cgatggcggg ctggtcctcc
    12361 tgaaggacgt ggcccgggtc gagatcggcg cgcagtccta cagcacgatc ggccgctaca
    12421 acggccagcc ggcgtccggc atggcgatcc agctcgccag cggcgccaat gcgctggaga
    12481 cggcggaccg ggtgcgcgcg cgcatggacg agctcgccgc gttcttcccg gaaggggtgg
    12541 tctacaacgt cccctacgac acgacgcctt tcatcagcat ctcgatcgag gaggtggtga
    12601 tcaccctctt cgaggcgatc gtgctcgtct tcctcgtcat gttcctcttt ttgcagaact
    12661 ggcgggcgac gctcattccg accctcgccg tgccggtcgt catcctcggc acgttcggcg
    12721 tgctggccgc cttcggcttc tcgatcaaca cgctgaccat gctcgccatg gtgctggcca
    12781 tcgggctgct cgtcgacgac gccatcgtgg tcgtcgagaa tgtcgagcgc atcatggagg
    12841 aggaggggct cgacccgcgc gcggcgacca ggaagtcgat gggccagatc accggggccc
    12901 tgatcggcat cgcggtcgtg ctctccgccg tcttcgtgcc gatggccttc ttcggcggct
    12961 cgaccggcgt gatctacaag cagttctcca tcaccatcgt ctcggcgatg ttcctctcgg
    13021 tggtggtggc gctggtgctc accccggcgc tgtgcgccac catcctcgag tcccggacct
    13081 cgcaccgcaa gcagcgcggc cccatcggct ggttcaaccg cttcttcgac ggcacgcagc
    13141 gcggctatgc ctcgagcgtc ggctggatcg tccgccggcc ggtccgggtg ggcctcgtct
    13201 acctcctgct cgtcgccggc atgatcggcc tcttcgcccg cacgcccacc ggcttcctgc
    13261 cggacgagga ccagggcatc atgttcacct tcgtgcaggg gccggccggc gccacggcga
    13321 cccgcacgct cgacgtgctg gaccaggtcg agagccattt cctcgagaac gaggccgaga
    13381 acgtcgattc gatgttcacg gtggtgggct tcagcttcgc cggctccggc cagaatgtcg
    13441 gcctcggctt cgtcaggctg aaggactggg acgagcggcc gcgagccgat caggacgtgc
    13501 acgccatcgc cgggcgggcg ttcggcgcca tgatggccat ccaggacgcc atggtcttcc
    13561 cgatcgtccc gccagcggtg atcgagctcg gcaatgtttc cggcttcgac ttctacgtcc
    13621 aggcgcgggc cgggcagagc caccagcagc ttctcgaggc ccgcaaccag ctcatcggca
    13681 tggcgtcgaa gagcccgctc atcggctcga tccggcccaa cgggctggag gacgcgccgc
    13741 aatacgggct cgacatcgac tggcgcaagg ccggggccat gggggtcacg gcgaacgacg
    13801 tgagcgacct tctgaccgtc gcctgggccg gccgctacgt taacgacttc ctcgatcggg
    13861 gccggatcaa gcgggtctat gtgcagggcg aggccgatgc gcggagcacg cccgaggaca
    13921 tcgagcgctg gcgggtgcgc aacgccagcg gcggcctcgt gccgttctcg aacttcatcg
    13981 agggcggctg gcgcttcggc ccgcaggggc tctatcgtta caacggcgtc ccggccatgc
    14041 agcttcaggg ctcgccggcg ccgggcgtgt cgaccggcga ggccatggcc gagatcgagc
    14101 ggctggtggc ggagctgccg ccgggcttcg acatctcctg gaccgggctc tcgctcgagg
    14161 agcaggaatc gggcaaccag gcgccgctgc tctatttcct ctcggtcgcc gtggtgttcc
    14221 tgagcctcgc cgccctctac gagagctggg tggtgccggc cgccgtcatg ttcgccatgc
    14281 cgatcggcgt gctcggcgcg ctggtcggcg cctggatcgg cggcttcgag aacggcgtct
    14341 tcttccaggt ggggctgctc accaccatcg ggctgaccgg caagaacgcg atcctgatcg
    14401 tcgagttcgc ccgtgcccgg ctggaggagg gcgaggagct gctgaccgcc gtcatcgagg
    14461 cggcgcggca gcgcttccgg ccgatcatca tgacgtcgat ggccttctcg ctcggcgtgc
    14521 tgccgctcgt cctgagcagt ggtgccggct ccgccggccg gcgggcgatc ggctcgggcg
    14581 tgctcggcgg cacgatcacg ggcacggtgc tcggcatcct cttcgtgccg ctcttcttcg
    14641 tgctgatccg ccgcctggtg ccccggcgcg aggcggcgaa ggcggcggcg ccgctgccgg
    14701 cggcgtaggc aaggggccgg cccgccgcgc tggcctccgg cggccgggcg ggctatgagc
    14761 ggccgcaggc gcgccacgga cggggaggaa gacgcatggg cgagacgcag gtcatcaaga
    14821 agcggcggct cgggcgaacg gggctcgagg tcacgcccat ctgcttcggc acctcggggc
    14881 tgggcgacat gccgagcacc tatggctaca gcgtcgacga ggagcggggc cgggccaccg
    14941 tgcgggcgat cctggcgggg ccggtgaact tcctcgacac ctcgcgcaac tacgccatgg
    15001 ggcggagcga gcagcggatc ggcgcggtga tccgggagct gggcggcctg cccgagggct
    15061 tcgtcgtctc gaccaagctc gatcgcgaca tggagacgcg ggagttcgac gcggcgcgcg
    15121 cccggcgctc gctcgaggag agcctgaagg cgctgcatct cgaccgggtc cagctcctgc
    15181 acctgcacga tccggagcac gcggccgatc tcggcccgat cacgcgcaag ggcggggcga
    15241 tcgccgagct cttccggatg aaggaggagg gcctgtgcga cgcggtcggg ctcgccgccg
    15301 ggcggatcga catcatgatg ccgatgctga aggactggga attcgacgcg ctgatcaccc
    15361 acaaccgctt taccctcacc aaccgcaatg ccctgccgat gctggagctg gcgaaggaac
    15421 ggggcatcgc catcctcaac gcggcgccct atgccggggg cgtgctggcg aaggggacgg
    15481 acagcttcaa gcgctatgtc taccaggagg catcggccga ggtgctcgac ccggtgcggg
    15541 cgatcgaggc ggtctgcgcc aggcacggcg tgccgcccgg ggcggcggcg ctccagttct
    15601 cgatgcgcga cccgcgcgtc agctcgacca tctgcggcgt caccaggccc gagcgggtga
    15661 cccagacgct ggaatgggcc gccttcccga tccccgacgc cgcctgggac gagctgctgg
    15721 cgctgccgtt cgccacggac gacccggagg cgacgcgggt ctacgatccg gggtgaggtc
    15781 gtgggctctc aggcaaacgg gttggtgagc cgcacacctt cgatcacctg accgttggcc
    15841 atatcctcgg tgtagagcgt gcggcagccg gcctcggcgg ccgaggcgat gatcagcgcg
    15901 tcgtagatgt ggtagccgta gcgctcggcg atgttcaagg cggcgtcgtg ggtggtggcg
    15961 gtgagcggcc ggggctcgcc gcacagggtg ccgagagtcg cgagggccgt gcggatatcg
    16021 gtccaggtcg aagctcgctt gcgccgggcg acactgatga actcgttcag cacctgaatg
    16081 ctgacgatgc cgccattggc gagcaggcgc tcggccacct cgctgcggtc gccggaatcg
    16141 atgaaggcgt agacgacgag gttggtgtcg aagaacgcct cagcggtcat tcggaagatc
    16201 gcgactgccg cgctcgtgcg cttcctcacg atcgaagcgg tagtcgggtg gcagcggggc
    16261 gctgcgtcgg cgtagctgcc gcatcgtctc caacgccttc tcccgctgct cgtcgcgagc
    16321 gatctcaacg ctcctggatg tggcgacgcg gatctccacc tcgtcgcctt caacgagcgc
    16381 caactctctc accacccgtg ctgggaggcg caacgccaag ctgttgcccc atcgagccac
    16441 gcgcatatac caaccccaat gctatacacg aatgagaatg tatcgcattg gcgagccgag
    16501 cgcaagccgg cctcaatcct cgatcctgcc gccgtgctcg tcctcgatgt gggcctggat
    16561 gatctcgtcg atcgaggcgt cggcctcgta gcccatgtcg cgggcgcgct tcgaggcgaa
    16621 gttgtagggc cagccgtcga cgatcttctg gatcttcggg tcgggcacct gcttgacgcg
    16681 gtcggcgacc ttgtcgccgg cgatgcggcg gagcgcctcg agcgcgtcgc cgatcctgac
    16741 cgcgatgccg ggcagggtca ggctgcggac ctggccgaag gcgtcgctgg gcaggccggc
    16801 ggtcttgacg aaggcgtcga tcacgcggcg cggcgagagg atgtacatgg tcgagtcggg
    16861 gtcgaccggg cagatcgccg cctggccggc gaggggttcc ctgatgatcg agctggcgaa
    16921 ggtcgaggcc gccttgttgg gcttgcccgg gcggacgacg atggtgggca ggcggagcga
    16981 gcggccgtcg atgtagccct tgcgcgtgta gtcggcgacc aaaagctcgc aggccgcctt
    17041 ctgcgcgccg taggaggtct gcggggtgag gtggaagtcg tcgccgatga gcgccggcgg
    17101 ggtcgggacc gggccgccga agacggcgag cgtgctggcg aagacgaggc gcgggcaggt
    17161 gccgagcgcc ttcaggccct cgaggaggta gcgggtgccg tcgagattga ccttgtagcc
    17221 gaggtcgaag tcctcctcgg cattggcgct gacgatggcg gcgaggtgga cgacgaggtc
    17281 gtagccctgg gcgatgagct tcgccgcggc gacgtgatcg gtgatgtcga gcgcgaccct
    17341 ggcgaggcgc gcgtcgtcgg ccaggaggtc gaggccgtag aggtcggcgg cggtgatgtg
    17401 ctggacggtc cggccgtcga ccgtgccgtc ggcgaggagc cgctcggtga gcttgcggcc
    17461 gatgaagccg ccggcgccgg tgatcaggat acgcaaggta agggctcctt ctggtgcacg
    17521 ccgggtcgcg tggcgcgggt gatgtctgtg ttgcagggaa gggccgcctg aggcaaggtg
    17581 cgcggagtca gagggacgac ccggcagaag ccgggaccac gatcaggggg tacgataatg
    17641 acgtttcgct tcacgcgccg ggattcgatc aagctgggcg ccggtgtcgg cgccgccgcc
    17701 ctgctcggca gcacggccct gcgcgccgag attcccacct acgacgccac cgagccggat
    17761 ttgccgatcg aggacggcgc tgccctgcgc gtgctccggc cgtcgaagtt cgtcgccggc
    17821 gacgaggagc tcttcaacca gaacagccag gccttcactg ccgccaccgg cgtcgatgtc
    17881 cgcatcgact acgagggctg ggaggatctc cgcccgaagc tggcggtcgc ggccaatgtc
    17941 ggccagggtc cggacattgc gctggcctgg tcggacgacc cgcacaagtt cgtcgacagc
    18001 tgcctcgacc tgaccgacgt cgccgaatat ctcggcgaga agtacggcgg ctggtatccg
    18061 ctggccgagc gctacggcac caatggcggc aggtggatcg ccatgccgat gggtgcttcg
    18121 ggcggccgcg tcgtctatcg caagtcgatc atcaacgagg ccggctacga tgccgtgccc
    18181 ggcgatgtgg acggcttctt gcagatgaac cagaagctcg ccgcaatggg caagccgggc
    18241 ggcctcgccc tcggcaacgc cgtcggcgac ggcaatgcct ggtgcaactg gctggtctgg
    18301 gccttcggcg gggcgatggt cgacgagaac gaccaggtga tcatcaacag cccggagacg
    18361 atcgaggcgc tgaagtacgg caaggcgctc tacgacacgt tcatcccggg cacgctctcc
    18421 tggctcgacc cgtcgaacaa caaggcctat ctggccggcg agctgagctg gacccagaac
    18481 ggcatctcga tctactacgc cgccaagaac agcgaggatg cggccgtcaa ggcgatcgcc
    18541 gaggacacct accactcgcg aatgccgatc ggcccggtcg gcatgacgac cgagcgctgc
    18601 ctcatggtca acgccatggt gctcaactac acgccctatc cgaacgcggc gaagcgctat
    18661 ctccagttca tgatggagca cgagcagtac gaccagtggc tgaccgccgc gatcggctac
    18721 tggggccatc cgctcaaggc ctacgaggcg tccgagatct ggtcggtcga tcccaagcac
    18781 gaggcgttca agagcgtgat cgccgattcg ctctgggacg gctacaaggg cagcatcggc
    18841 gaggccagcg ccgccgtgct ctccgacttc gtcgtggtca acatggtggc ctcggtctgc
    18901 gccggtcagg cgacgcccga ggaggccgcg gccgaggccg agcggcgggc caagcgctac
    18961 taccgcggct gacgggcggt cgaacgactg tggtgccgtc accggcgcgg tgacggcacc
    19021 atgtgtctcg aggggaagga aggccggcgt ggcagccacc tccgacactt atgttgcgca
    19081 gacgcggccg gacaactggc tcgcccggct gttcgacaac aaggcgttcc tgatcgtcgt
    19141 ctgcatgacg ccggcggtcg ggctgctcgt gctgttcctg acctacccgc tcggcctcgg
    19201 cgtctggctc gccttcaccg acacgcggat cggccgggcc ggcgagttcg tcggcttctt
    19261 caactacgag ctcctgttct ccgaccgggt cttctggatg gcggtctggt ggacggtctt
    19321 ctacacggtc ttcgcgacga tcttcaagtt cgcgctgggc ctctggctgg cgctgctcct
    19381 gaaccactac gtgccgttca agtccgtggt ccgggcgatc atcctcgtcc cctggatcgt
    19441 gccgaccgtg ctctccgcca tcgccttctg gtggatctac gacccgcagt tctcgatcat
    19501 ctcctacgtg ctggtcgacg tgctcggctg gcgggagaac tatatcgact tcctgggcga
    19561 cccctggaac gcccgctggt cgctgatcgc cgccaatgtc tggcgcggca tccccttcgt
    19621 cgccatctgc ctgctcgccg ggctgcagac catctcgccc tcgctctacg aggcggcgat
    19681 gctcgacggc gccacgccct ggcagcgctt cgtccatgtc accgtgccga tgctgatgcc
    19741 gatcctcgcg gtcgtgctca ccttctcggt gatcttcacc ttcaccgact tccagctcgt
    19801 ctacgccatc acccgcggcg ggccggtcaa cgccacccat ctcatggcga cgctggcctt
    19861 ccagcgggcc attccgggcg gccagctcgg cgagggggcg gcgatcgccg tcgccatgat
    19921 ccccttcctc atcgccgcca cgttcttcag ctatttcgcg ctggcccggc gcaagtggca
    19981 gcagggaggc gacgatgact gaggcggccc gggccatcga ccagccgccc gcggtgaaca
    20041 ccggcgagcg ccagggggcg atcagctggg aatcgctgcc gcgccgcatc gtcacgatct
    20101 acacgccgct cgtgatcttc atgatcgtcc tgctgttccc gttctactgg atgacgatca
    20161 ccacgttcaa atcgaacgag gagctctaca actacaagga ctacaacccg ttctgggtga
    20221 gctcgccgac gctcgccaac atcaacaagc tgctgttcga gaccgactac ccgacctggc
    20281 tgatgacgac catgaccgtg gccgtggtct cgaccatcct ctcggtcttc gccagcgtgc
    20341 tggccgccta tgccatccag cgcctgcgct ttcggggctc acagtgggtc gggctctcga
    20401 tctacctcgc ctacctggtg ccgccctcga tcctcttcat cccgctcgcc accatggtct
    20461 tccagttcgg gctcttcgac agccagctcg ccctgatcct cacctatccg accttcctca
    20521 tcccgttctg cacctggctg ctcatcggct acttcaagtc gatcccctac gagctcgagg
    20581 aatgcgcgct catcgacggc gcctcgcggc tgcagatcct gatcaggatc accctgccgc
    20641 tcgccgtgcc cggcctgatc tcggccggca tcttcgcctt caccctctcc tggaacgagt
    20701 tcatctacgc gctcgccttc atccagtcgg ccgagaacaa gaccgtgccg gtggcggtgc
    20761 tcacccagct cgtcgagggc gacgtctacc actggggctc gctgatggcc ggcgcgctcc
    20821 tgggctcggt gccggtggcg atcttctact cgttcttcgt cgagtattat gtctcgagcc
    20881 tgacgggtgc cgtgaaggag tagctgtttc tccgcgagcg attgccagac gcaggttggc
    20941 gcaaccttga cttgaaccgc cctctcgccc ctagttcggc ccaaggaaac gcagccttcg
    21001 ggggaggccg gacatgggca ggaagcggct cgtggtgtgc tgtgacggca cctggaacga
    21061 gccggtcacg cgcaccaata tctggcggct gaagaacgcc attgcctcgc gcgaggtgct
    21121 ggcggaccgc gccgagattc cgcagtgggt gtactacgat gccggggtcg gcaccgagcg
    21181 gggcaaccgg ctgaccggcg gcatgctcgg catggggctc tcgcgcaatc tcaaggaggc
    21241 gtatcgcttc ctcgccgaat cctatgccga gggcgacgag atctggtgct tcggcttctc
    21301 gcgcggcgcc tacacggcgc gctcgctctg cggcttcctc ggtgttgccg gcctgctgcg
    21361 gcatcgcgat ctcggccttc tggacgacgc cttcgaatac taccgcctgc cgaaggcgaa
    21421 gcgggaggcc agccgcttcg gccggatcag ggacgacctc catctgcaca cgaaggagaa
    21481 gtgcctgagc gtgcggctgc tcggcgtgtt cgacacggtg ggggcgctgg gcgtgccgct
    21541 gccctggctc aaggcgctga cccagtggcg gtccgtgcag ttccacgaca cggagctgtc
    21601 gagcctgatc gagaacgcct accaggcctt ggcgatcgac gagcgacggg ggccgttcca
    21661 gccgacgctc tggaccggcg cgccgggcga ggcgcggtcc tgcgacggcc ggatggtgcc
    21721 gcagcgggtc gtccaggcgt ggttcccggg cgtgcattcc gacgtgggcg gcggctatga
    21781 cgagaaggcc tatgccgacg tgacgctcga ctggatgctg gacgtcgccc gctcgctcgg
    21841 cctggtgctg cgcgaggatt atgtcgagcg gcagctcagg cccgatcacc tggcatccct
    21901 gcacgaatcc tacaccaccg gctggcgcct cgcggaccgc atccccgggt tcaccagggg
    21961 aggtgcgcgg ccgatcggca acgagcagcg gcgggctcgg gggcgtggcc cgatagcgcc
    22021 gaccgaggtg ctgcactgga cggcgtcggc ccgggccgcg gcggccgggg cgcggctgcc
    22081 gtccggccgc tcgccctact ggcccctgaa cctgtgcgac ggcgaggggg ccctgcatcc
    22141 gacgctcgcg gggctgcggc acgagaagcg gcgggcggcg cgacggctgc tcgacgaggt
    22201 ctgcacgctg gacggccggg cggccgagct ggtcgatctc tcgcgcgccg gcgcccgggt
    22261 ccggctcgcc gccggcacgc cgccgccctc cggggcgggt gcgctgcggc tggtgagccg
    22321 cacgacgggg gcgcgtctcg gcagcgtcgt ctggcgacgc gaggggctga tcgggctcgt
    22381 cttcgaggat gccgcctgag acgtcctacc cgtggcgggc gcggctgtcg gccggggctt
    22441 ccgcgcgatc cgtcaggccg tagtcgcggc tgacctcggc gaccctgagg cggtagtcgg
    22501 cgaagatctg gccgcggccc cgggcctgcg ccaggcggtg ctcggcgagg ctgcgccagg
    22561 cggcgacggc ggcctcgtcg cgccagaagg agagcgagac gaacttgccg ggctcgctca
    22621 cactctcgaa gcgttcgacc gagatgaagc cgtcgatctg ctcgagcagg ggacgcagct
    22681 cggcggcgag ctcgaaatag gcgtcccgct gctccggctt cggccagagc tcgaagatca
    22741 cggcgatcat cggcgcgctt cctgctggta gaggaccacg gaacgggccg gcagcgatcg
    22801 ggtgctcgcc tcgccgctgg tgtcgaggcg gaccgaccag tcgcccggcg gcaggtgcag
    22861 ctccagggcc acgcggccgg cattgagcgc caggacgaag cgggcgtcca gggacgggtg
    22921 gccgtcatcg tcgatctcgg tcggcgcgtc gccggccagc gccaggagga tggcgcgcgc
    22981 ggcggggtcg tgccagtcgt cgggctccag cgccgtgccg tcgggcttca gccactgcac
    23041 gtccggcgtg ccgtcggcgg cggcgacgct gccgtggagg aagtggcggc ggcgcagcac
    23101 ggggtgtgcc cggcggatcg ccgcgagctc ggccgtgaag gcgatgaggt cggtgtcggc
    23161 ggtggcccag tcgagccagg agagcgcgtt gtcctggcag taggcgttgt tgttgccctg
    23221 ctgcgtgcgg cccagctcgt cgcccatggc gagcatcacc gtgccctggc cgaggaagag
    23281 ggtggcgagc aggttcctga cgtcgcggcc acgggcggcc agcacggcgg ggtcgtcgct
    23341 cggcccctcg acgccgttgt tccaggagag attgtggccg tggccgtcgc cattcgcctc
    23401 gagattggcc tcgttgtgct tcatttcgtg ggccacgagg tcggccaggg tgaagccgtc
    23461 atgggcggcg acgaagttga tcgaggacca ggggcggcgg ccctgatgct cgaagagatc
    23521 cgccgagccg aggaggcggg cggcgagctc gggcagcagg ccctcgtcgc cccgccagaa
    23581 gccgcgcacc acgtcgcggt agcggtcgtt ccattccgac cagccggggc cgaagccgcc
    23641 catgcggtag ccctcggggc cgatgtccca gggctcggcg atcatgcgca catgggcgag
    23701 caccgggtcc tgcttcaggc agtcgaagaa cccggccccc ttgtcgaagc ccaaaggctc
    23761 gcgcccgagc gtggcggcga gatcgaagcg aaacccgtcg atgcccacca cctcgaccca
    23821 gtagcgcagg gaatccatga tgagctggag cacgcgcggg tggctggcgt cgacggtgtt
    23881 gccgcagccc gtgaggttgg cgtagccgtc ggccgcctcg gcatcgagac ggtagtagct
    23941 ggcattgtcg aggccgcgaa aagcgaggca ggggccctgg ccgtcgccct cgccggtgtg
    24001 gttgtagacg acgtcgagca ggacctcgat gccggccgcg tgcagggcgg ccaccgcctc
    24061 gcggagctcg cccagcggat cggcggtggc ggcatagcgg ggttcgacca tgaaccagtt
    24121 gatcgggttg tagccccagt agttggtgag gcccttctcg atcaggtgcg gctcgtcgac
    24181 gaaggcctgg accggcagga gctcgagggc ggtgacgccc aggcgctgca ggtgctcgac
    24241 gacacgtggg tcggcgaggg cggcgtaggt gccgcggcgg tcggccggaa ccccctcgtg
    24301 caggcgggtc aggcccttga cgtgggcctc gtagatcagt gtctccggcc gaccgttgcg
    24361 gggtcgggac caggcgccgg ggacatgctg gtcgggcccg gcgacgactg ccttggcgac
    24421 gtggggtgcc gtctcggcgc cgaagcggca ggcggcgccg ccggggcgga gggcggcccg
    24481 gtcgaaggcg ccaacggcgt ggcgggcgta ggggtcgagc aggagcttcg ccgggtcgaa
    24541 gcgatggccg gccgccgggt catcgggccc gtgggcgcgg tagccataga cgaggccggg
    24601 gccggcatcg ggcaggaagc cgtgccagac ctcgtcggtc cattccggca gggtcagccg
    24661 ggcctgctcg atgcggccgg tcgggtcgaa gaggcagagc tcgaccgtcg tggcatgggc
    24721 ggagaagagg gcgaagttga ccccgccctc ggtcgccgtg gcgccgagcg gccaggggtg
    24781 gccggggcag aggcggcggg ccgggctcgt cgcctcgggc cggccgggat tggtggcgag
    24841 ccggttcacg cgatatgact gacgggtgga cgtggctgcg gggctatggg caatgaccga
    24901 aactctccag ggcgaggttg agccggggcc gggactccgg tccgggcagg cgtgggccga
    24961 ccagggtgac gccatgctga tcgatcatcc cgacacattg caagcaccgg ccgagggggc
    25021 gtgatggagg gcgacccggc acgcgcgctc gacgagctgc gccgccggct ggcgccgctc
    25081 ctggagcagg cgggccccgg gcggccggcg ctcgaggcgc ggctcgaggc cgtcttcgag
    25141 cagctcttcg cggcgctttc cgcgctctac gggcaccgct gggacttcgc ctggcagctc
    25201 gaggcggtgc tgcggacggc gctggaggca gcgctcgcgc ggccggagcg gctggcggcc
    25261 cgggacaggg agcgccggcc cgactggatc cgcgagagcc ggcaggtcgg ggccatgggc
    25321 tatgtcgacc gcttcgccgg gagcatcgtc ggcctcttcg accggctgcc gcatctggag
    25381 catctggggg tcacctacct gcacctgatg ccgctcttcg ccgtgccgga cgggcccaat
    25441 gacggcggct acgcggtctc gagctatcgc gaggtggcgc cccggctggg cagcatggcg
    25501 cagctcgccg atctcgccgc cgcgctgcac gaccggggca tggcgctggt gctcgacttc
    25561 gtcctgaacc acacggccga cgaccatgcc tgggccgaag cggccaaggc cggcgacccg
    25621 gcgatgcagg ccttctactg gatgttcgac gagcgtggcg aggtcgagcc cttcgcccgg
    25681 agcctgcggc agatctttcc cgatcgcggc ggcgacgcct tcgtctggcg ggacgacgtg
    25741 gccgggggga agtgggtctg gagcacgttc tatcccttcc agtgggacct gaactacacc
    25801 aaccccgagg tgctccgggc gatcctgggc gagatgctct tcctcgccaa ccagggggtc
    25861 gacgtgctcc ggctggatgc cgtgccgttt ctctggaaga aggccgggac cccttgcgag
    25921 aacctgcccg aggcgcatct ggtggtccgc gccttcaatg cggccgcggc gatcgcggca
    25981 ccctcgctgg tcttcaagtc cgaggccatc gtccatcccg acgaggtcgc ccgctacgtg
    26041 cgcgtcgacg agtgccggct ctcctacaac ccgctcctca tgtgctcgct ctgggacgcg
    26101 ctggcgaccc gcgacacgcg gcatctgcgc gacgtgctgg cgcggcggct ccgcacggcc
    26161 gaggggacag cctgggtcaa ctatctccgc tgccacgacg atatcggctg gggcatggcc
    26221 gacgaggaca tgacggagct cggcatcgcc cccgaggatc atcgccgctt cctcaacgcc
    26281 ttctacaccg ggcgcttccc cggcagcttc gcccgcgggc tgccgttcca ggagaacccg
    26341 cgcacgggcg actgccgggt ctcgggcacc tgcgcggcgc tggccgggct cgagcaggcc
    26401 gaggagctgg gcgacgcgac gctgctcgag catgcggtgc gccggatcgt gctgctgcac
    26461 gggctggtct acaccatggg cggcatcccg ctcctctatc tcggcgacga gatcgcccag
    26521 cccaacgatc cctcgtacac gagccggccg gccgaggccg ccgacaatcg ctgggtgcac
    26581 cggcctttct tcgacgacgc acggctgcag cgggcgctcg ccgagccggc gacgccggcg
    26641 gggcgggtgc tggaagcgct ggtgcggctg aaggaactca ggcgctccga gccggcgctg
    26701 gcggtggggc cggtcgagct gctcgagctc gacccgcgct acacgctcgc ctatcgcaag
    26761 cgggcccagg ggcgcagcct gacggtcgtc gccaacctct gcgagcacga ggtcgacatc
    26821 gaggcgggcg ggctcggccc cgggcccttt ctcgacctct ggggcggccg aaacctcgag
    26881 ccggcccggc tccgctcgct cgaggcctac gggctgctcg tcctgaggcg ggacggctga
    26941 cgggagcgag ggcggcagac gcgcggcacg ccctcgcgag ccggatcagc gatacaggct
    27001 cttgtccatc aggttgggat agaggcccgc cacctcctcg gcatagccgt tgaagaggag
    27061 ggtcgggacg tcctcctgcg tgccgagcag gcgctcggtg gcctggctcc agcgcgggtg
    27121 cggcacggcc gggttgacgt tggcccagaa gccgtactcg ttgccgtcca ccacgttcca
    27181 gaacgtgtcg ggccgctcct cgacgaggct gattcgctcg atcgacttga tgctcttgta
    27241 gccgtacttc cagggcaggg ccaagcgcag cggcgcgccg ttctgcctgg gcagcggctt
    27301 gccgtagagc ccggtgacca tgaaggctag gtcgttgccg gcctcggcga gggtgaccgc
    27361 ctcggtgtag ggccagggat accagaaggc ccgctggccg ggggccacgg cggaatcctc
    27421 gaacgtctgg aacagcacgt agcgagcgct cgccagcggc tcggcgaggt cgagcagatg
    27481 gcgcagcgcg aagccgctcc agggcacgat catcgcccag gtctcgacgc agcgatgccg
    27541 gtagacccgt tcctcgagcg gcatgcgccg caggaggtcg tcgatgtcga tggtcatggg
    27601 ctcggcgacc aggccgtcca ccgtcacggt ccagggccgg gtcggcaagg cctgggcggc
    27661 gcgctggatg ttcttgtgcg agccgaactc gatgaagttg ttgtagccgg cggcgagccg
    27721 ctcgggcgtc catggctcgg gcaggtcgaa cgcggcattg cgcgccgccg gatagagcgc
    27781 ggccgaaggg tcggccggtg cggccgcctc ggtcgcggcg acggcttgcg acgcctcttc
    27841 cttgcaaccc gcgacggcga ggccggcggc ggccgccatg ccgccgatca gccgacgccg
    27901 gccgaaggcc agacgctcgt cggtgacgag gctctccgcg agcttccaac gtggccgacg
    27961 cgagggcagg agcacggcgc aaccatccgc tgattggtga tattccggat acggttagca
    28021 gctcgcaacg gatttgtctc gccggcgtga tttcctcacg ggatcgcgac cggtcgcgag
    28081 cggtgggacc tgacatcctg gcaggctctg ccattttgtc atggcctgcc attttgtcag
    28141 gatggtcgat ttgcgccgtg gttccaagag gttggccatc gacggccccg gcgctcctga
    28201 cattccgtca tgaatgccct tcggcacaca ccggccagct cgaggaattt ttctcgataa
    28261 aacagatagt tatcaagaaa tcgagagctg gcacggtgct tgcttggctc tcgacgtgaa
    28321 acaacgagtc gggtgggcag catgcgcttc ctgggggcac tgagcttggg tctggtgggg
    28381 ccggtcgtgg tcgcgccgct ggcggtcgcc ttgccgatcg cggggacggc gccttcggca
    28441 cggccggaag gggcgccgac gatcgagcaa ttcgatccgc ccgagggctg gatggagcgg
    28501 gccttgcgag ggatcagcga gccgatcccc ggcggcctgg agttcctgca ggaccagggt
    28561 gcgtggttca cgcccttcaa tcatccgaat gcgaccgggc gatacgacat tcgaggcctc
    28621 cacggcagcg aggggacacc ttccggataa ggcgaacaat ggcctgtcgg aacggcaggg
    28681 tgagccgttc cggccggccc gagctcgttc ggcgagcagc cgaacggtcg gatgggggga
    28741 gcatccgcat cgtgaaccgg ccctcaagaa ctcccgacga ttcgcgggcc aatgtcacag
    28801 ggaaaagttg gatgtcgagc aagaaagagc ggaatctgac gctggcggcg ctgtgcacgg
    28861 ccgcgctgtt ggctctgccg gtcgccacat ccgcacaggg tatgggcggc atgtcgggca
    28921 tgggtggcat gggtggcatg tccggcatgg gcggcatggg cggcatgtcc ggcatgggcg
    28981 gcaacagcat cggccagtgg gtctgcacgc agtggacctc gaccggcatg ggcggcatgt
    29041 cgggcatggg tggcatgtcg ggcatgggtg gcatg
//
LOCUS       DAOTFI010000020        28882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1842_length_28882_cov_6.880078, whole genome shotgun sequence.
ACCESSION   DAOTFI010000020 DAOTFI010000000
VERSION     DAOTFI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 28882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28882
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_28882_cov_6.880078"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2049)
                     /gene="addB"
                     /locus_tag="P5558_03225"
     CDS             complement(<1..2049)
                     /gene="addB"
                     /locus_tag="P5558_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HRY23355.1"
                     /translation="MGRLLSIPAERPFLASLARTLLREPAELLADHLILLPSRRACLE
                     LRDVFLELSPAPALLLPRLQPVGEVDLGDLPLPLAGSVPDLPPPIDPLRRRLLLARLV
                     KAKDPMPDEQAIRLATALAELLDMVQTEQADLGRLAALLPGDLAEHWQEVVKFLGLLD
                     EAWPTLLQAEGASDPAAWRNARLEAAAAALLATPPAGPVIAAGITGTVPAVADLLAAV
                     LELDGGMVVLPGFDPLIDGASYEAIGPTHPYYPFRLLLDRLGRRPGDVAFWPDPAPAR
                     AAPARTAFLAEIMRPAATAEAWQNLELPAAALAGLEIAECDGHAEEALLVALKLRAVL
                     EQPGRRAALVTPDRQLARRVAAELARWQIEVDDSGGVPLDQTPPGSFILLTAHAFLGD
                     GGPVPLLSALKHPLARGGLAPGGLRRLARLLDRRVLRGARPAAGIRPLIEALPDDVDG
                     GLRDWLAELAAAADPLLATAAAGGPFPALIEAHLAFVEWLAADATGDARELWAKETGE
                     AARDFLARLLEAADGEAAPAPSAYPAVLAVLMGAEAVRPRGRSHERLMILGQLEARLV
                     EADTVVIAGLNEGTWPRTGEPGPWLNRHLRQALGLPPVELSIGIAAHDLFMAASAPEV
                     LFTRAAKDPGGAPTVASRWLQRLDAVTKAVEQRHLLQPALDWQDWQRRLDAPEGPITP
                     C"
     gene            complement(2060..2536)
                     /gene="tsaE"
                     /locus_tag="P5558_03230"
     CDS             complement(2060..2536)
                     /gene="tsaE"
                     /locus_tag="P5558_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRY23356.1"
                     /translation="MPGLRHELACATRDATLVFARRLAGVLRAGDTLLLEGDLGAGKS
                     EIARAIIQELMGAAVEVPSPTYTLVQHYTAPQGGILHADLYRLNGADELVELGLDDAL
                     GQVLLLVEWPERAGPGWFPASSLTLKIAIGQGEARVLQLCGDAAWADRLARLINHS"
     gene            complement(2542..3837)
                     /gene="ahcY"
                     /locus_tag="P5558_03235"
     CDS             complement(2542..3837)
                     /gene="ahcY"
                     /locus_tag="P5558_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRY23357.1"
                     /translation="MSQFTDFKVADIGLAGWGHREIAIAEKEMPGLMALRDEYGDSQP
                     LEGARIVGCLHMTIQTAVLIRTLVHLGATVRWSSCNIFSTQDHAAAAIADSGIPVFAW
                     KGETEEEYEWCIEQTIEGPDGWTPNMILDDGGDLTLVMHEKHPDLMKAVKGISEETTT
                     GVLRLYDMARKGKLMCPAINVNDSVTKSKFDNKYGCRESLVDGIRRATDVMMAGKVAV
                     VAGYGDVGKGSAESLRHAGARVMVTEIDPICALQAAMDGYEVVTMEEAATRADIFVTA
                     TGNKDIITLDHMRAMKDGAIVCNIGHFDNEIRVADLKNLKWTEIKPQVDQIEFPDGKQ
                     ILLLAKGRLVNLGCGTGHPSFVMSASFTNQVLAQIELWTNTGRYENKVYVLPKHLDEK
                     VAALHLDKLGVQLTRLSADQAAYIGVQPQGPFKAEQYRY"
     gene            complement(3952..4242)
                     /locus_tag="P5558_03240"
     CDS             complement(3952..4242)
                     /locus_tag="P5558_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRY23358.1"
                     /translation="MSDAVPDAWRQSCTIVNARGLHARAAAKLVRLAGEHDAQVLVEK
                     DGMQVPATSILGLMMLAAGRGSTLMLEARGRDAEAAIAALVGLVERGFDESD"
     gene            complement(4239..4655)
                     /locus_tag="P5558_03245"
     CDS             complement(4239..4655)
                     /locus_tag="P5558_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY23359.1"
                     /translation="MIGLVLVTHGRLAEEFAAAMQHVVGPQSQIAAISIGADDDIEQR
                     RQDIVAAVEAMDTGKGVIILTDMFGGTPSNLAISTLERPGVEVIAGVNLPMLIKLAGV
                     RDQGDLESAVEAAQDAGRKYINVASRLLAPGKATAG"
     gene            complement(4675..5661)
                     /gene="rapZ"
                     /locus_tag="P5558_03250"
     CDS             complement(4675..5661)
                     /gene="rapZ"
                     /locus_tag="P5558_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRY23360.1"
                     /translation="MSTPAGSSTISRRRLVIVTGMAGAGRTSALKRLEDQGFEAIDNL
                     PIPLIERFLRLEEGLERDLAIGIDSRTRAFDPRRLVELVEGLADANPDVAVTLLFCDC
                     DDEALQRRFTETRRRHPLAADRPVQDGIAAERALLAPLRAAADEVIDTTQLSLPELHR
                     LLAGHFPQAGDAPGLMVSLVSFAYRRGLPREADLVLDVRFLQNPHYVAGLKPLTGLDP
                     AVQAHIRSDPGFDTFMARLEALVMPLLPRYRAEGKSYLTIAIGCTGGQHRSVFVTVLL
                     ADTLRRAGYEVSTSHRELAAPGEVPATAPGAATPGVGTGVAFGRGTGASGGA"
     gene            complement(5658..6116)
                     /locus_tag="P5558_03255"
     CDS             complement(5658..6116)
                     /locus_tag="P5558_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HRY23361.1"
                     /translation="MAVLEGEDESGDVIVHATAIRWQEHGILLRGPSGAGKSDLALRL
                     IERGALLVADDLVRIGLDAAGRLMARSPGEPGLIELRGQGIFRQPALALTRLDLCLDL
                     VADATLALDRLPAPATALYCGRRLPLYRLEPFAASATARLAVLLAGERVA"
     gene            complement(6274..6741)
                     /gene="ptsN"
                     /locus_tag="P5558_03260"
     CDS             complement(6274..6741)
                     /gene="ptsN"
                     /locus_tag="P5558_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HRY23362.1"
                     /translation="MIELSDLLPPGSVVLGLKAGSKRQVLRVLAERVAADEGLDPAKV
                     LDALLEREKLGTTGIGDGLAIPHAKLDGLDRLVGMFARLDPPVDFEALDDEPVDLLFL
                     LLAPTAAAADHLKALARVARVFRDEALCRALREVEEPGRAFELLTGRRRPQAA"
     gene            complement(7004..8431)
                     /gene="rpoN"
                     /locus_tag="P5558_03265"
     CDS             complement(7004..8431)
                     /gene="rpoN"
                     /locus_tag="P5558_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRY23363.1"
                     /translation="MALGLRLDLRQTQSLVLTPQLQQAIRLLQLSNHELIGTLRSETE
                     ENPFLEIAETAASPKQAPAPVAQPSARPERDNAGDWDQPRPPRALDQQLHGPRRDPDA
                     AGQGSFEARLSELPSLRDELRRQIGMLTADPGLRRLAFDLCDWVDDEGYLREPDALIA
                     ERTHAGIERVAAARALLQRCEPTGIGGRDLAECLALQLAERDRLDPAMRRLLDNLPLL
                     ARADWPALQRLCGVDRDDLEEMVAELKALDPRPGHAFASEPQVTLLPDIVVYRTGPDA
                     WRIELNPEALPRLVVHGDYHAELTRSGLDLRQREFVNERYQNATWLARALDQRSRTIL
                     KVSRAIFARQRAFLDDGPRALRPLVLRDIAAATGLHESTVSRATSQKYVATPHGTFPL
                     KYFFSTAIASNDGGAEHSAEVIRLQIKRLIHDEDPQAVLSDDQIVGRLEGLGIAIARR
                     TVAKYRESLGIASSTQRRRSKALGR"
     gene            complement(8544..9401)
                     /gene="lptB"
                     /locus_tag="P5558_03270"
     CDS             complement(8544..9401)
                     /gene="lptB"
                     /locus_tag="P5558_03270"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRY23364.1"
                     /translation="MAAELDVPPRLRPLGARESEPPGAAAGVIHVPEANGAARAPARL
                     PAGRLEAVGLMKAYGGRVVLRDVSFRIIPGEAVGLLGPNGAGKTTSFYMVTGLVAADG
                     GRILLDSVDVTALPMHRRARLGIGYLPQEASIFRGLSVADNIRAVLEVAVPQKALRHR
                     RLDELLGEFGLTHLRDAPASTLSGGERRRCEIARALASDPRYILLDEPLAGIDPLNVA
                     EIRQLVGHLKDRGIGVLITDHNVRDTLDIIDRAYILADGVVIKEGAPAEIVADPAVRR
                     VYLGDSFER"
     gene            complement(9406..9963)
                     /gene="lptA"
                     /locus_tag="P5558_03275"
     CDS             complement(9406..9963)
                     /gene="lptA"
                     /locus_tag="P5558_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HRY23365.1"
                     /translation="MPARFRWLDGQWRAGWRRLALAGSLALLPALAHAQAENGLGAHD
                     TSQPIEIVADRLTVQQADGRAVFEGNVVAEQGELALSAARLIVYYQQGQNDAAPQQAI
                     RRIEVEGDVSIASPSETATGDSGYYDVVGGEIVLTGNVVLTREANVVTGDRLEIDVAR
                     QLATMSTTDSAKGRVRALFQPSAGG"
     gene            complement(9963..10904)
                     /locus_tag="P5558_03280"
     CDS             complement(9963..10904)
                     /locus_tag="P5558_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRY23366.1"
                     /translation="MLHGQAVLRTEAAAIDQLALGLGAPFAAAVALMATLEGRVVCTG
                     MGKSGHVARKIAATLSSTGRPAMFVHPGEASHGDLGMITTKDAVLALSNSGETAELRD
                     LILYTRRFAIPLIAMVGRAASTLATEAEVALVLPELTEACPLGLAPTTSTTAMLALGD
                     ALAVALLERAGFTPQHFAVFHPGGKLGSQLMRVEQLMRGAGELPLVATDCPMSEALVV
                     MTEKCLGCVGVADDGGRLLGIVTDGDIRRRMGPDLLGRRAGELMTRGPRTIAPDALAV
                     EALTIMNRHAITVLFVLRDEVPVGAVHLHDCLRAGIA"
     gene            complement(11247..11855)
                     /locus_tag="P5558_03285"
     CDS             complement(11247..11855)
                     /locus_tag="P5558_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRY23367.1"
                     /translation="MSVTLHVDDLPDGLELGSVVAVDTETMGLLPHRDRLCVIQLSAG
                     DGNAHLVQLTGGKAPRLGAVLADPARLKLFHFARFDLAVLRHHLGIDARPLYCTKIAS
                     RLVRTYTDRHGLKDLCRELLGIDLSKAEQSSDWGAPALSEAQQRYAAQDVLHLHALKE
                     RLDPMLEREGRAALAAACFDFLPTRAALDLAGWADIDVFAHS"
     gene            complement(12008..12094)
                     /locus_tag="P5558_03290"
     tRNA            complement(12008..12094)
                     /locus_tag="P5558_03290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12058..12060),aa:Leu,seq:cag)
     gene            12275..13216
                     /locus_tag="P5558_03295"
     CDS             12275..13216
                     /locus_tag="P5558_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HRY23368.1"
                     /translation="MRNQVVTVFGGTGFIGRQLVQHLAGLGAALRVPTRDPDRAGFLR
                     PMGDVGQIAILPASADPEGVARLVHGTAAAVNLVGILHERRDGDFLKVHRDFAGTVAT
                     AAKAAGTRRLVHLSAIGADPDGAARYARSKGEGEAAVRRACPEATILRPSIVFGPGDG
                     FFTRFARMSMLSPVLPLIEGGRTRFQPVYVGDVVEAITTCLADDVGRGKTFELGGPRI
                     ASFEELLRYLLATLHRRRLLLPVPARLAAFQARFLEYLPSPPLTRDQIRLLQKDNVVA
                     PGALTLADLGIEPTPLETVVPRYLAPFKRQQPRLRPA"
     gene            13517..14986
                     /locus_tag="P5558_03300"
     CDS             13517..14986
                     /locus_tag="P5558_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23369.1"
                     /translation="MAARMMQFTDVPQAMPNKRAADERAQDFGEIYGRYDERRAADQA
                     SRCEQCGIPFCQVHCPLHNNIPDWLRLTAEGRLEEAYELASATNNMPEICGRICPQDR
                     LCEGNCVIEQAGHGTVTIGAIEKHITDTAFDMGWVQPVRPLHERPESVGIIGAGPAGL
                     AAAEELRREGYQVRVYDRYDRVGGLLIYGIPGFKLEKPIVERRAELLRQGGVAFELGA
                     DIGGAGAALGFAELRRRHDAVLIATGVYKARALAAPGVGQDGVVAALDYLTASNRKGL
                     GDSVPAFDAGTLDARGKHVVVVGGGDTAMDCVRTAVRQGATSVSCLYRRDRANMPGSA
                     REVGHAEEEGVEFVWLAAPEAFLGDGKVRAVRAFRMRLGAPDATGRQTPEPVEGSTFE
                     RPADLVIAALGFDPEDLVTLFDEPELPVSRWGTVKIDWRTMMTALPGVFAAGDIVRGA
                     SLVVWGVRDGREAAAGIHRHLQSSERAGADSAAALALAS"
     gene            14998..19539
                     /gene="gltB"
                     /locus_tag="P5558_03305"
     CDS             14998..19539
                     /gene="gltB"
                     /locus_tag="P5558_03305"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRY23370.1"
                     /translation="MSQSSIEQALATYQRERAKLEALGYEAEAFERDACGVGCVVAID
                     GRPRREVVVAGIDALKAVWHRGAVDADGKTGDGAGIHIQIPQDFYKGQIRGMREADHH
                     VAVGMVFLPRNNIHQQEACRTIVESEVLRFGYVIRGWRQVPVDTSVIGEKAAATRPEI
                     EQIIIGNPIDGDGPNDSEAFERDLYLIRRRIERLALAESLADFYICSLSCRSIIYKGL
                     FLAEQLATFYPDLQDERFVSSFAVFHQRYSTNTWPSWKLAQPFRTLAHNGEINTLRGN
                     VNWMKSHETRMVADIFGEHNEDLKPVIQAGSSDSASLDAVAEALVRAGRSLPLVKTLL
                     VPPAWSNKMAMPQAHQDLFSYCNCVMEPWDGPAALVATDSRWVIAGMDRNGLRPLRYS
                     RTSDGLLVVGSEAGMVPLKEVDIVEKGRVGPGEMIGVDLVTGTFFRDVALKDRLAAHR
                     PYGKWVENIRLLEDLVAARPPAPVHYGREELRRRQALFGLTIEDMELILSPMVLDAKE
                     AVGSMGDDTPLAVLSKGYRGLHHFFRQQFSQVTNPPIDPLREWRVMSLKTRFGNLGNI
                     FDEDPSQTRILQLETPMLTTDDFRALEAYFGANGRTIDCTFKAKGERLREALARIRQE
                     AEEAVRGGCEQLILSDEAVGPERAPVPMILATSAVHSHLVRQGLRAFSSLTVRTGECL
                     DVHYFAVLIGVGATTVNAYLAEAAIADRHARGLFPGLTLAECLDRYKEAVGQGLLKIM
                     SKMGISIISSYRGGCNFEAVGLSRSLAREYFPGLITRISGMGLTAIKNKVRAQHERAY
                     AEAEPALAIGGFYRYRRSGERHAYEARMVHQLQQAVATDSYVAYQRFSDMVQAGDPVS
                     IRDLLDFRQGRTPVPLDEVESITEIRKRFVTPGMSLGALGPEAHETLTIAMNRIGAKS
                     DSGEGGEGVERYTPRPNGDNANSPIKQVASGRFGVTAEYLNAARELEIKVAQGAKPGE
                     GGQLPGFKVTVEIARLRHATPGVTLISPPPHHDIYSIEDLAQLIYDLKQINPDAKVCV
                     KLVSEAGIGTIAAGVAKAKADVILISGHVGGTGASPQTSIKYAGSPWEMGLSETNQLL
                     TLNRLRHRVTLRTDGGLKTGRDIVIAAMLGAEEFGIGTLSLVAMGCIMVRQCHSNTCP
                     VGVCTQREDLRAKFTGTPEKVVNLMSFIAEEVREILASLGLRSLEEAVGRSDLLKQVG
                     RGSEDLDDLDLNPILAQVDPGGLPRHCTLKGRNEVPDSLDAQMLKDARALLEDGEKMQ
                     LQYNVRNVQRAVGTRLSAHITRRYGMKGLQPGHLTVRLRGSAGQSLGAWAVQGLKLEV
                     FGDANDYVGKGLSGGTIVVRPTVSSPLVADQNFIIGNTCLYGATDGKLFAAGRAGERF
                     AVRNSGAMTVVEGCGDNGCEYMTGGVAVILGAVGDNFAAGMSGGMAYVWDAAGQLEAH
                     LNPDMVIMQPVLVDHYVQELKNLLVEHVWQTQSTLAERILHDFDIELPRFRQIVPKEI
                     LDKLPVPVQPQADAEARLA"
     gene            19536..20837
                     /locus_tag="P5558_03310"
     CDS             19536..20837
                     /locus_tag="P5558_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRY23371.1"
                     /translation="MRRFLVLALALALAGPALATPPVPASRDAINEALDGIQSLPLPL
                     PEAGELRVGRPADERRLNRGFLEDLAAQPDWPDQGIVIAAGRYRLEDVATELDRPELL
                     ACETDTCRLAAPLAVEHGAVLVIEGLTVELEQRAGAAIVAFGDLFISLSAIEGRAGDA
                     PATTDGEAFRPFIVAFDRSRTVIRGSRLAALGYDGNGATGLSVTVTSRDDPPARPVLQ
                     LVGNLIEGMYDGVQVRGAAEATLLRNRIRASLRHGIAIRDGTADGLIADNDIVGSGAM
                     ADNGNGLLLSRGITRTAVLANRIIDSAGAGIQIDKGAFDLTLAGNELARSGRDGLVLY
                     ESWSVDLQRNAIAQNGRSGIRIRAADGILVSENGLTGNARAGIDLQDWSTVTKPPSEE
                     EAALIRPTEVTVKGNRFADNARGDCVVQGVVTILPAEGSDC"
     gene            20831..22135
                     /locus_tag="P5558_03315"
     CDS             20831..22135
                     /locus_tag="P5558_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRY23372.1"
                     /translation="MLRRATLATLAFLAFASSTLAQVPTPAEIAALADEILAEPVPAP
                     LAGEVALAEAANEPALRRGFLDELEALPGWQGVAIVLRSGRHRLEALPARIDRPDLLA
                     CDGRRCRLAAPLVVAAGAVLVIDGIELRLAQETGALVSAHGGLFISDSELLGWNDEAD
                     MPARTDDRGTAFRPWITGLEDNRTVVRRSRLAHLGYQGTSTFGLAFTHGGRDPAEGGP
                     RVSVVANAIEDLYFGFFSFGAVGVEVIQNEISRSHVYGVDPHDATRDMLIAQNTVTGT
                     RRSHGIILSREIHDAMVVLNQSSDNAGAGLFIDKGSHDVTFARNRAFANGKDGIVVYE
                     SRDITITRNELFANGRAGIRVRASADVRVSGNAIRHDAGPGIFVYDWSHAAREPDEED
                     ATHLLPTSVELSSNSLANNAGGDCSIQGEVEIRIPPNGYSDC"
     gene            complement(22144..22353)
                     /locus_tag="P5558_03320"
     CDS             complement(22144..22353)
                     /locus_tag="P5558_03320"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRY23373.1"
                     /translation="MTVERAILLVVGVLVLVTVLLAVWQSLNWLWVTGLLGAHLIQAS
                     FTGVCPVVMTLKRMGLPTKAGFAQA"
     gene            complement(22421..23257)
                     /gene="ppk2"
                     /locus_tag="P5558_03325"
     CDS             complement(22421..23257)
                     /gene="ppk2"
                     /locus_tag="P5558_03325"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRY23374.1"
                     /translation="MGKKKHAAKASPAAEAAAPEKMKRKVFEQELHELQVELTRLQAW
                     VKAAQQRIIVVFEGRDTAGKGGVISRITGRASPRVFKHVALPAPSDRERTQIYAQRYI
                     QHFPAAGEIILFDRSWYNRAGVERVMGFCTDEEYERFLALTPAFEREMIVNSGIILRK
                     YFLDVSQGEQRRRFGARIKEPLKHWKLSPMDTESVRRWWDYTAAYQRMIAATHAPEAP
                     WHIVPADDKRRARLNLIRHLLDSIPYEKVPPELPKMPKAQSRPKGAREGLGAGELVPA
                     HY"
     gene            complement(23345..23809)
                     /locus_tag="P5558_03330"
     CDS             complement(23345..23809)
                     /locus_tag="P5558_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY23375.1"
                     /translation="MRRGILVAGAVAIAAALGAAALWRGLGEPATRSAASPEMLALGA
                     ALYGEHCASCHGVELEGQPDWQRRLSSGRLPAPPHDASGHTWHHADSILFRIVRDGPA
                     AIVGGAYESDMPGFADLMTDAEIEAVLAFIKSTWPERERAVQEQASIADDPA"
     gene            complement(23806..24246)
                     /locus_tag="P5558_03335"
     CDS             complement(23806..24246)
                     /locus_tag="P5558_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HRY23376.1"
                     /translation="MRNLLFVAVLGLAVGSTANGHAEEPSRSVELIKSPSCGCCAAYG
                     DYLEASGFEVTTRPVEDIVGLSRLAGIPDDFQGCHLAMIDGYFVSGHVPVEVVERMLD
                     ERPAVRGITLPGMPAGSPGMMGEQLEPFRVFAIGAGGPELYADE"
     gene            complement(24359..24703)
                     /locus_tag="P5558_03340"
     CDS             complement(24359..24703)
                     /locus_tag="P5558_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23377.1"
                     /translation="MRAGVRSLSLVIMCLFIMGTMMHVAAVAMTAGVAGLPSGHVDSA
                     MDGGCPDCAMPQDAMMACADVCAGPLTAIPSEAALAGASGRLSPWPSGSALPRGRPGL
                     PEPHPPKSPVTS"
     gene            complement(24764..25750)
                     /locus_tag="P5558_03345"
     CDS             complement(24764..25750)
                     /locus_tag="P5558_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HRY23378.1"
                     /translation="MKGELPPVRPFELDPRAGRFETVEETPELPAELAPPPVPPARPF
                     WRRPGLLLVGSIIGLVLLQAIDYVTGLIETTPLLGWPFAVLLAVVVVTALGTLWKEVF
                     DLRRVRRAERLRLTGQRLAGSELHGEAVGYLDDLDQQLGERHKAEIRAASERGLDDSE
                     VLSLYERQVLAPRDKRAYRLVLEASRDIGLLTALSPLGLMDGLLVLWRATLMIRGIAR
                     AYGLALGSAAAFNLLRRCIRNAAIAGLADVVSHAAVEHVGASLAALLSARAGQGAGNA
                     LLAGRLGLEAIRLTRPLPFIAEPPPRLSHVRAAIFEPGGKIDPVQTRPERGE"
     gene            complement(25747..27105)
                     /locus_tag="P5558_03350"
     CDS             complement(25747..27105)
                     /locus_tag="P5558_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HRY23379.1"
                     /translation="MLGLDRLGGFVRDLPGEVAELGDRLVDRHLRLAVTGLRRSGKTV
                     FTTALVHHLLDGHDLAFVGAVHEGRYRGARLLPVRGPAFPYATFHRTLMADRPTWPDA
                     TDRLTVLKLELRFRTTGLVQRLMAPNGSLILEIIDYPGEWLLDLPLLEMDYPTFSETA
                     AQRDRVEPRAAVAGEWRAALAGLSPTTTPAELVEVYRRYLGRVQNELGLSLVQPGRLV
                     TAGADPATGFAPLPDAAPAELRRAMAARFETYKRQVVEPFYTDHFSRFDRQIVLVDLF
                     HTLNRGRAHFADSRDALETILESFRYGTNSLLARLFRPSIEKLLFAASKADHVAANQH
                     ANLKQLLELMIAPARRKARFAGLETDVLALAALRATDTVRTEHQGQLLSCVRGRLADE
                     GRETVLFPGEIPGDLPDDEDWRSGRFRFRDFEPRRLEPGVKGQHLRLDQAIEFLIGDK
                     LR"
     gene            complement(27124..28047)
                     /gene="ubiA"
                     /locus_tag="P5558_03355"
     CDS             complement(27124..28047)
                     /gene="ubiA"
                     /locus_tag="P5558_03355"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRY23380.1"
                     /translation="MSGQAGEPVVRLPDEEYGWLVRLPRGWRDYGLLARLDRPIGTWL
                     LFIPCLWGLALAGGTPAMLWAPLMAIGAIAMRGAGCTINDMADRDFDRRVARTAGRPL
                     AAGRLRMKQAMAFLALQLAIGLGVLLFLPLTAALIALCSMPLVIGYPFMKRITYWPQV
                     FLGLTFNWGVLVGYAASMGGLGLPALFLYLAGIAWTLGYDTIYAHQDKEDDALIGVKS
                     TALLFGEATRPWLYGFYAAMLGLLVLAGWSAGLGPLFFLALLPVAGLLAWQVQAVDLD
                     DPADCLDRFRSHRGLGLLVGVALLLGHWQLP"
     gene            28098..28832
                     /locus_tag="P5558_03360"
     CDS             28098..28832
                     /locus_tag="P5558_03360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRY23381.1"
                     /translation="MRDTRLHLEAPLGPGASLDLEPEAAHYLRAVLRARAGRTLRVFN
                     AGSGEWRARLVRLDRHRATLAVETQLRPPAAEPGPTLLFALLKKPRLEWLLEKAVELG
                     AARLQPVRCRHAVVEAARPDRLRQRLIEAAEQCERLSLPELLPETGLLEAVHAASEPG
                     VILADEAGGAALAPTLRRYPEAALLVGPEGGFATDERTALRALTFVRPVSLGATILRA
                     ETASLMLLACHRALTSAADRPCRAEA"
BASE COUNT         4315 a        10297 c        10215 g         4055 t
ORIGIN      
        1 gcagggcgtg atcggcccct ccggcgcatc cagccgccgc tgccagtcct gccagtcgag
       61 cgccggctgc agcaggtggc gctgctcgac cgccttcgtc acggcatcca ggcgctgcag
      121 ccagcgcgat gccacggtgg gcgccccgcc cggatccttg gcggccctgg tgaagagcac
      181 ctcgggcgcc gaggcggcca tgaagaggtc gtgcgcggcg atgccgatcg agagctcgac
      241 cggcggcagg ccgagcgcct ggcgcagatg ccggttgagc cagggccccg gctcgccggt
      301 gcgcggccag gtgccttcgt tgagcccggc gatcaccacc gtgtcggcct cgaccagccg
      361 cgcctcgagc tggccgagga tcatcagccg ctcgtggctg cggcctcggg ggcgcaccgc
      421 ctcggccccc atcagcaccg cgagcacggc cggataggcg ctcggcgccg gcgccgcctc
      481 gccatcggcc gcctccagca gccgggccag gaaatcgcgc gccgcctcgc ccgtctcctt
      541 tgcccagagc tcgcgcgcat cgccggtcgc atcggcggcg agccattcga cgaaggccag
      601 atgcgcctcg atcagggccg ggaaggggcc gccggcggca gccgtcgcca gcaggggatc
      661 ggccgctgcc gcgagttccg ccagccagtc gcggagcccg ccatcgacat cgtcgggcaa
      721 ggcctcgatg agcggccgga tgcccgcggc cgggcgggca ccccgcagca ctctccggtc
      781 gagcaggcgg gcaagacggc ggagcccccc cggggcgagc cctcctcggg ccaatggatg
      841 cttcagggcg gagagcaacg gcaccgggcc gccgtcgccg agaaaggcgt gcgcggtcag
      901 caggatgaag ctgccgggcg gggtctggtc gagcggcacg ccgccgctgt cgtccacctc
      961 gatctgccag cgggcgagct cggccgcgac ccgcctcgcg agctgccggt ccggcgtcac
     1021 cagcgccgcc cggcggccgg gctgctcgag cacggcccga agcttcagcg ccaccagcag
     1081 cgcctcctcg gcatgcccgt cgcattcggc gatctcgagc cctgctagcg ccgcagcggg
     1141 cagctccagg ttctgccacg cctcggcggt tgccgccggg cgcatgatct cggccaggaa
     1201 cgcggtgcgg gccggggccg cccgcgccgg ggccgggtcg ggccaaaacg ccacgtcgcc
     1261 gggccggcgg ccaagccggt cgagcagcag gcggaaggga taataggggt gggtcgggcc
     1321 gatcgcttca tagctcgccc cgtcgatcag cggatcgaag cccggcagca cgaccatgcc
     1381 gccatcgagc tcgagcaccg ccgcgagaag atcggccacc gccggcaccg tgccggtgat
     1441 gccggcggcg atcaccggac ccgccggcgg cgtcgccagc agggccgccg cagccgcctc
     1501 caggcgggcg ttccgccagg ctgccggatc ggatgccccc tcggcttgca gcagcgtcgg
     1561 ccaggcttcg tcgagcagac cgaggaattt caccacttcc tgccagtgct cggccaggtc
     1621 gcccggcaga agggccgcga gccggccgag atcggcctgc tcggtctgga ccatgtcgag
     1681 gagctcggcg agggcggtcg cgaggcggat cgcctgctcg tccggcatcg ggtccttggc
     1741 cttgaccagc cgggcgagca gcaggcggcg gcgcagcggg tcgatcggcg gcggcaggtc
     1801 cggaacggag ccggcgagcg gcagcggcag gtcgccgaga tcgacctcgc ctaccggctg
     1861 cagccgcggc aggagaagtg ccggtgccgg cgagagctcg aggaagacgt cgcgcagctc
     1921 gaggcaggcg cgcctggacg gcagcaggat cagatgatcg gcgagaagct cggccggctc
     1981 gcgcagcagc gtcctcgcga ggctcgcgag gaacggccgt tccgccggga tgctcaggag
     2041 cctgcccatg ggcacaactc tagctatggt tgatgagccg cgccagccgg tccgcccagg
     2101 cggcgtcgcc gcaaagctgc aggacgcgcg cctcgccctg gccgatggcg atcttgagcg
     2161 tgaggctgct cgccgggaac cagcccggcc cggcccgctc cggccattcg acgagcaaga
     2221 gcacttgccc gagcgcgtcg tcgagaccga gctcgaccag ctcgtcggcg ccgttcagcc
     2281 ggtagaggtc ggcatgcagg atgccgccct gcggtgcggt gtagtgctgc accagcgtgt
     2341 aggtcgggga cggcacctcg acggcggcgc ccatcagctc ctggatgatc gcccgggcga
     2401 tctcgctctt gccggcgccg aggtcgcctt ccaggagcag cgtgtcgccg gcgcggagca
     2461 cgcccgccag ccgacgcgcg aacaccaggg tggcgtcgcg cgtcgcgcag gcgagctcgt
     2521 gccggagacc cggcaaaggt gtcagtagcg gtactgctcg gccttgaacg ggccctgcgg
     2581 ctgcacgccg atataggccg cctggtcggc cgagagcctg gtgagctgca ccccgagctt
     2641 gtcgagatgc agggccgcca ccttctcgtc gaggtgcttg ggcagcacgt agaccttgtt
     2701 ctcgtaacgg ccggtgttgg tccagagctc gatctgcgcc agcacctggt tggtgaagga
     2761 ggcgctcatg acgaagctcg gatggccggt gccgcagccc agattgacca gccggccctt
     2821 ggccaggagc aggatctgct tgccgtccgg gaactcgatc tggtcgacct gcggcttgat
     2881 ctcggtccac ttgaggttct tcaggtcggc gacgcggatc tcgttgtcga aatggccgat
     2941 gttgcagacg atggccccgt ccttcatcgc ccgcatgtgg tcgagggtga tgatgtcctt
     3001 gttgccggtg gcggtgacga agatgtcggc gcgggtcgcc gcctcctcca tggtcaccac
     3061 ctcgtagccg tccatcgccg cctggagcgc gcagatcggg tcgatctcgg tgaccatcac
     3121 ccgggccccg gcgtgacgca ggctctcggc ggagcccttg ccgacatcgc cgtagccggc
     3181 gaccacggcg accttgcccg ccatcatcac gtcggtggcg cggcggatgc cgtcgaccag
     3241 gctctcgcgg cagccgtact tgttgtcgaa cttcgacttg gtgacgctgt cgttgacgtt
     3301 gatggccggg cacatgagct tgcccttcct ggccatgtcg tagagccgga gcacgccggt
     3361 cgtcgtttcc tccgagatgc ccttcaccgc cttcatcagg tcgggatgct tctcgtgcat
     3421 gaccagcgtc aggtcgccgc cgtcatcgag gatcatgttc ggagtccagc cgtccggccc
     3481 ctcgatcgtc tgctcgatgc accactcgta ctcctcctcg gtctcgccct tccaggcgaa
     3541 gaccgggatg ccgctgtcgg cgatggccgc cgcggcatgg tcctgagtcg agaagatgtt
     3601 gcaggacgac cagcggaccg tcgcccccag atggacgagg gtgcggatca gcaccgcggt
     3661 ctggatggtc atgtgcaggc agccgacgat gcgcgcgccc tcgagcggct ggctgtcgcc
     3721 atactcgtcg cgcagcgcca tcaggcccgg catctccttc tcggcgatcg cgatctcgcg
     3781 atggccccag ccggcgaggc cgatatcggc cactttgaaa tcggtgaatt ggctcatgtg
     3841 ttcaggtctt tccggtgact gtccaaggcc caattggggg catggcacga gaatgcgagc
     3901 ccgccggagc gaaacacgca gctttgtccc gcggcatggc cacagtcggg ctcaatcgct
     3961 ttcgtcgaag ccgcgctcca ccaggccgac aagggcggcg atggccgcct cggcgtcgcg
     4021 cccgcgcgct tctagcatca gtgtcgagcc gcgaccagcc gccagcatca tcagtccgag
     4081 gatggaagtc gccggcacct gcatcccgtc cttctcgacc agcacctggg cgtcatgctc
     4141 gccggcgagg cgcacgagct tcgcggcggc gcgggcatgc aggccgcgtg cgttgacgat
     4201 ggtgcagctc tgtcgccatg catccggcac ggcgtcgctc aacccgccgt ggccttgccc
     4261 ggcgccagga gccggctcgc cacgttgatg tacttgcgcc cggcgtcctg ggcggcttcg
     4321 acggccgact cgagatcgcc ctggtcgcgc acgccggcga gcttgatgag catcggcagg
     4381 ttgaccccgg cgatcacctc gactcccggg cgctcgagcg tcgagatggc gaggttggag
     4441 ggcgtgccgc cgaacatgtc ggtgaggatg atgacgccct tgccggtatc catcgcctcg
     4501 accgcggcga cgatgtcctg ccggcgctgc tcgatgtcgt cgtcggcgcc gatgctgatc
     4561 gccgcaatct gcgactgcgg gcccacgaca tgctgcatgg ccgcggcgaa ttcctccgcc
     4621 agccggccat gcgtcaccag taccaagccg atcatcgtcc cccgccgccc tgtctcacgc
     4681 gccgccagag gcgccggtgc cgcgtccgaa ggcgacgccg gtgccaacac ccggcgtcgc
     4741 agcgccgggt gccgtggccg gcacctcgcc cggcgccgcc agctcgcggt gcgaggtgct
     4801 tacctcgtat cccgcccgac gcaaagtgtc ggccagaagc acggtcacga aaaccgaccg
     4861 gtgctggccg ccggtgcagc cgatggcaat ggtcagatag ctcttgccct cggcccgata
     4921 gcgcggcagc agcggcatca ccaacgcctc cagccgtgcc atgaacgtgt cgaagccggg
     4981 gtcggagcgg atatgcgcct ggacagctgg gtcgaggccc gtcaagggct tgagccctgc
     5041 cacataatgc gggttctgaa gaaaacgcac atcgagcacg agatcggcct cacgtggcag
     5101 gccacgccga taggcgaagg agacgaggct caccatgagc ccgggcgcat ccccggcctg
     5161 gggaaaatgc ccggcgagga ggcggtggag ctcgggcagg ctgagctggg tggtgtcgat
     5221 cacctcgtcg gcggccgccc gaagaggggc gaggagcgcc cgctcggcgg cgatgccgtc
     5281 ctgcaccggc cgatcggccg cgagcggatg gcgccgacgg gtctcggtga agcggcgctg
     5341 cagcgcctcg tcgtcgcagt cgcagaagag gagcgtcacc gcgacgtcgg ggttggcgtc
     5401 ggcgagcccc tcgaccagct cgaccaggcg gcgcgggtcg aaggcgcggg tgcggctgtc
     5461 gatgccgatg gccaggtcgc gctcgagccc ctcctcgagc ctgaggaagc gctcgatcag
     5521 gggaatcggc aggttgtcga tcgcctcgaa gccctgatcc tcgagccttt tcaaggcact
     5581 ggtgcgaccg gccccggcca tgccggtaac gatcaccagc cgccgccggc tgatggtgct
     5641 gctgccggcc ggggtgctca agcgacccgc tccccggcga gcagcacggc gagcctggcc
     5701 gtggccgagg cggcgaatgg ctcgaggcga tagagcggca ggcggcggcc gcaatagagg
     5761 gcggtcgccg gcgcgggcag ccggtccagg gcgagggtcg cgtcggccac cagatcgagg
     5821 cacagatcga gccgggtcag ggcgagcgcc ggctgacgga agatgccctg gccgcgcagc
     5881 tcgatcagcc cgggctcgcc gggcgaccgg gccatcaacc gaccagcggc gtcgagcccg
     5941 atccggacga gatcatccgc gaccagcagc gccccgcgct cgatcaggcg gagcgccaga
     6001 tcgctcttgc cggcgcccga cggaccgcgc agcaggatgc cgtgctcctg ccagcgaatc
     6061 gcggtggcgt ggacgatcac gtcgccgctc tcgtcctcgc cttcgagcac agccatgccc
     6121 gggccgttca ccgggccggc gggcagacca cgcgcatcag ctcgcatggc gcgaccttcg
     6181 ccctggctgc cgcgcatgga atggccacgg gcacggacag ctcccccgcg ggcagcggcg
     6241 agcggcagtt ggcatcggtg cttgcgccat ccgtcaggcg gcctgcggcc gccgccgccc
     6301 ggtcaggagc tcgaaggcgc gccccggctc ctcgacctcg cgcagcgccc ggcagagcgc
     6361 ctcgtcgcgg aacacccggg cgacccgggc gagggccttg agatgatcgg ccgcggcagc
     6421 ggtcggtgcc aggagaagaa aaagcaggtc gaccggctcg tcgtccaggg cctcgaaatc
     6481 aaccggcgga tcgaggcggg cgaacatccc gaccagccgg tcgaggccgt cgagcttggc
     6541 atggggaatg gcgagaccgt cgccgatgcc ggtggtgccg agcttctcgc gctccaggag
     6601 ggcgtcgagc accttggcgg ggtctaggcc ctcgtcggct gccacccgct cggccagaac
     6661 ccgcagcacc tgccgcttgc tgcccgcttt gagcccgagc accaccgagc cgggtgggag
     6721 caaatcggag agctcgatca ttgcgatgct ttcaagggct gtctggcgct cgggagcggc
     6781 ctcgggaatc tgccggtcga tcgccggtgg ctggcacggc tggcagtggc acggcggccg
     6841 atcggcaaga cccttgccgc gtccacgagc cgtcgcggat tgtagcacgg tcggcccggc
     6901 tgcgtcgccg gggttgcccc ggctcgcccg gtgccgatcg gccgcgggca ccagggcgac
     6961 aggcttgccc cggggtccct gacgaagcgg ttggccacgc cgctcatcga cccagagcct
     7021 tgctgcggcg acgctgggtc gaggaggcaa tacccagcga ttcccgatac ttggcgacgg
     7081 tgcggcgggc gatggcgatg cccagcccct cgagccggcc gacgatctgg tcgtcggaga
     7141 gcaccgcctg ggggtcctcg tcgtggatca gccgcttgat ctgcaggcgg atgacctcgg
     7201 cgctgtgctc cgcaccgccg tcattgctgg cgatggcggt cgagaagaaa tacttgagcg
     7261 ggaaggtgcc gtgcggcgtc gccacgtatt tctggctggt ggcccggctc accgtgctct
     7321 cgtgcagccc ggtggccgcg gcgatatcgc gcaggacgag cggccggagc gccctcgggc
     7381 cgtcatcgag aaacgcccgc tggcgggcga aaatggcccg gctcaccttg aggatggtgc
     7441 gcgagcgctg gtcgagcgcc cgggccagcc aggtggcgtt ctgataacgc tcgttgacga
     7501 actcgcgctg ccggagatcg agacccgagc gggtcagctc ggcatggtag tcgccgtgca
     7561 cgacgagccg cggcagggcc tcggggttga gctcgatccg ccaggcatcg gggccggtgc
     7621 gatagaccac gatgtccggc aggagcgtca cctggggctc gctcgcgaag gcatggccgg
     7681 ggcgcgggtc gagcgccttg agctcggcca ccatctcctc gaggtcatcc cggtcgacgc
     7741 cgcagaggcg ctgcagcgcc ggccagtcgg cccgggccag gagcggcagg ttgtcgagca
     7801 gccggcgcat cgccgggtcg agccggtccc gctcggcgag ctgcagcgcc aggcattcgg
     7861 cgaggtcgcg gccgccgatg ccggtcggct cgcagcgctg caggagggcc cgggccgccg
     7921 cgacgcgctc gatccccgca tgcgtccgct cggcgatcag cgcgtccggc tcgcgcaggt
     7981 agccctcgtc gtcgacccag tcgcagagat cgaaggccag ccgacgcagc cccgggtcgg
     8041 cggtgagcat gccgatctgg cggcgcagct cgtcgcggag cgacggcagc tcgctcagcc
     8101 gggcctcgaa ggagccctgg ccggcggcat cggggtcgcg gcgcgggccg tgcagctgct
     8161 ggtcgagggc gcggggcggg cgcggctggt cccagtcgcc ggcattgtcc cgctccgggc
     8221 gggccgacgg ctgggcgacc ggcgccggcg cctgcttcgg cgaggcggcg gtctcggcga
     8281 tctcgaggaa cgggttctcc tcggtctcgc tgcgcagcgt gccgatgagc tcgtggttcg
     8341 agagctgcag aaggcgaatc gcctgctgca gctggggggt caggaccagg ctttgcgttt
     8401 gccggagatc gagacgaagc ccgagcgcca cgcggtcagc tccggcgggc ggacgagagg
     8461 cgaatcgaca ggcgggcggc ggtggttttg gacgtcgtca gcatcgccgg gtcagcacca
     8521 gtccgacagg tctgggaggt ggctcatcgc tcgaagctgt cgcctagata gacgcgccgc
     8581 acggccgggt cggcgacgat ctccgccgga gcaccctcct tgatgaccac gccatcggcg
     8641 agaatgtagg cccgatcgat gatgtcgagc gtgtcgcgca cattgtggtc ggtgatcagg
     8701 acgccgatgc cgcggtcctt gagatgaccg acgagctggc ggatctcggc cacgttcagg
     8761 ggatcgatgc cggcgagcgg ctcgtcgagc aggatgtagc ggggatcgga ggccaaggcc
     8821 cgcgcgatct cgcaacgccg ccgctcgccg ccggaaagcg tgctcgccgg cgcgtcgcgc
     8881 agatgggtca gcccgaactc gccgagcagc tcgtcgagcc ggcgatgccg gagcgccttc
     8941 tggggcaccg ccacctcgag cacggcccgg atgttgtccg cgacgctgag gccgcggaag
     9001 atcgaggcct cctgcggcag atagccgatg ccgagccggg cccggcggtg catcggcagg
     9061 gcggtgacgt cgacggaatc gagcaggatc cgcccgccat cggcagcgac caggccggtc
     9121 accatgtaga agctcgtggt cttgccggcg ccgttcgggc cgagcagccc caccgcctcg
     9181 cccggaatga tgcgaaagct gacgtcgcgc aggacgacgc gcccgccata ggccttcatc
     9241 aggccgaccg cctcgagccg gcctgccggc aggcgtgccg gggcccgggc ggcgccattc
     9301 gcctcgggga cgtggatcac cccggctgcg gcgccgggcg gctcgctctc ccgcgcaccc
     9361 aacggccgca agcggggcgg gacatcgagc tcggccgcca tgcgcctagc caccggccga
     9421 cggctggaac agcgcccgca cccggccctt cgccgaatcg gtcgtgctca tcgtggcgag
     9481 ctggcgggcg acgtcgatct ccagccggtc gccggtgacg acgttcgcct cgcgcgtcag
     9541 gacgacattg ccggtgagca cgatctcccc accgaccaca tcgtagtagc cgctgtcacc
     9601 ggtcgcggtc tcgctcggcg acgcgatgct cacgtcgccc tcgacctcga tccggcggat
     9661 cgcctgctgc ggcgccgcgt cgttctggcc ctgctggtag tagacgatca ggcgcgcggc
     9721 gctcagggcc agctcgccct gctcggccac cacattgccc tcgaacacgg cgcgcccgtc
     9781 ggcctgctgg accgtcagcc gatcggcgac gatctcgatc ggctggctgg tgtcatgggc
     9841 gccgaggccg ttttctgcct gggcatgggc gagagccggc agcagcgcca ggcttcccgc
     9901 gagggcgagg cgtcgccagc ccgcccgcca ctgtccgtcc agccagcgaa accgggccgg
     9961 catcaggcga tgcccgcgcg caggcagtcg tgcagatgca cggccccgac cggcacctcg
    10021 tcgcgcagca cgaaaagcac ggtgatggcg tgccggttca tgatcgtcag cgcctcgacg
    10081 gcgagggcat ccggcgcgat cgtccggggc ccccgcgtca tcagctcgcc cgcccggcgg
    10141 ccgagcagat cggggcccat gcggcggcgg atgtcgccgt cggtgacgat gccgaggagc
    10201 cttccgccgt catccgccac gccgacgcag ccgaggcact tctcggtcat caccacgagg
    10261 gcctcgctca tcgggcagtc ggtcgcaacg agcggcagct cgcccgcgcc ccgcatcagc
    10321 tgctcgaccc gcatgagctg gctgccgagc ttgccgcccg gatggaacac ggcgaaatgc
    10381 tgtggcgtga agcccgcgcg ctcgagcagg gccacggcca gcgcgtcgcc gagcgccagc
    10441 atcgccgtgg tggaggtggt gggggcgagg ccgagcgggc aggcctcggt cagctccggt
    10501 aggacgagcg ccacctcggc ttcggttgcc agcgtgctcg ccgcccggcc gaccatggcg
    10561 atcagcggga tggcgaaacg ccgcgtatac aaaatgagat cgcgcaactc ggcggtctcg
    10621 ccggaattgg agagggcgag caccgcgtcc ttggtggtga tcatgccgag atcgccgtgg
    10681 ctcgcctcgc cgggatggac gaacatcgcg ggccgaccgg tcgaggagag ggtggccgcg
    10741 atcttgcggg cgacatggcc gctcttgccc atgccggtgc agaccacccg gccctcgagc
    10801 gtggccatca gcgccaccgc tgcggcgaag ggcgcaccca ggccgagggc gagctggtcg
    10861 atggcggcgg cctcggtgcg cagcaccgcc tggccatgga gcaccgccgc ggcgtcgagc
    10921 gcctccgcgt cggccgggcc gtcgtgcgtc tcgctctcgc cgcgcagccg gacgctctgc
    10981 accgtcggct gggtcacgcc gtggctcctc tgctgccgtg tcggctcgtg ttccgcccca
    11041 tcaccaagag atcaggctcc cacagggccg gatcccgccg tccgggcctc cgcccgggca
    11101 ccagcggcgc cggccatcgt ggcgactggg ccggactatg ggcttcggca tccggccggt
    11161 caatcgccac ccggggcgct gcccggccac gatgccgcac gccggcgggc agcacccgtc
    11221 ggctggcccg ccgccctggc cggcgctcag gaatgcgcga aaacatcgat gtcggcccag
    11281 cccgcgaggt cgagggcggc acgggtcggc aggaaatcga agcaggccgc ggcgagcgcc
    11341 gcgcggccct cccgctcgag catcggatcg agacgctcct tcagcgcgtg cagatggagg
    11401 acatcctgcg ccgcgtagcg ctgctgtgcc tcgctcagcg ccggcgcacc ccagtccgag
    11461 ctctgctcgg ccttcgagag gtcgatgccg aggagctcgc ggcagaggtc cttgaggccg
    11521 tgccggtcgg tgtaggtgcg caccagccgc gaggcgatct tggtgcagta gaggggccgt
    11581 gcgtcgatgc ccaggtgatg gcgcagcacg gcgagatcga agcgggcgaa atggaagagc
    11641 ttgagccgcg ccggatcggc cagcacggca ccgagccgcg gcgccttgcc gcccgtcagc
    11701 tgcaccagat gggcgttgcc gtcgccggcc gagagctgga tcacgcagag ccggtcgcgg
    11761 tgcggcagga ggcccatggt ctcggtgtcg acggctacca ccgacccgag ctcgagcccg
    11821 tccggcaggt cgtccacatg gagggtcacg ctcatcgcag caaaaccacc gctccctgat
    11881 catccgccgg ctggggatcg cctgccatcc gggggtcggc cgatgtccgg gcctcgcgcg
    11941 aggactgcgg caccggcggc cgtcacaact catgcaacgg ccgcggtaaa ggtggcgtta
    12001 acgccgttgg tgcccaggag aggactcgaa cctccacgac cagaaggtca ctagcacctg
    12061 aagctagcgc gtctaccagt tccgccacct gggcttgggc ggtgcatcta tgaatgtttg
    12121 gccgccgcgt caagctcgcc ggcgcccggg cgtgcaacgc gcgccggcaa ccttgccggg
    12181 ggctgcgcgc tcggcgcatc ttgcatccga agccggcatt gccgacaaca cgcctgcgcc
    12241 gacaggcgac gccgcttcga ggaggattcc cttcatgcgc aaccaggtcg tcacggtctt
    12301 tggcggcacc ggcttcatcg gccgccagct cgtccagcat ctggccgggc tcggtgcggc
    12361 gctgcgcgtg ccgacccgcg acccggaccg ggcgggcttc ctccggccga tgggcgatgt
    12421 cggccagatc gccatcctgc ccgcgagtgc cgacccggag ggcgtcgccc gactggtcca
    12481 tggcaccgcg gccgcggtca atctggtcgg catcctgcac gaacggcggg acggcgattt
    12541 cctgaaggtc catcgcgact tcgccggcac cgtggccacc gccgccaagg ccgccggcac
    12601 gcgtcgcctc gtccatctct cggcgatcgg cgccgacccg gacggtgctg cccgctatgc
    12661 ccggagcaag ggcgaaggcg aggcggcggt gcgccgggcc tgccccgagg ccaccatcct
    12721 ccggccgagc atcgtcttcg gcccgggcga cgggttcttc acccgcttcg cgcggatgag
    12781 catgctctcg ccggtgctgc cgctgatcga gggcggccgg acccgcttcc agccggtcta
    12841 tgtgggcgat gtggtcgagg cgatcacgac ctgcctcgcg gacgacgtcg ggcgaggcaa
    12901 gaccttcgag ctcggcgggc cgcgcatcgc cagcttcgag gaactgctcc gctacctcct
    12961 cgccacgctg catcggcgcc ggctgctgct gccggtgccg gcgcgccttg ccgccttcca
    13021 ggcacgtttc ctggaatacc tgccgagccc acccttgacg cgcgaccaga tcaggctgct
    13081 gcagaaagac aacgtggtcg ccccgggagc gctgacgctc gccgacctcg gcatcgagcc
    13141 gacgccgctc gagaccgtcg tgccgcgcta tctggcaccg ttcaagcggc agcagccacg
    13201 actgcggccg gcctgacgga gtagtcgcga aaagcgacct gtttccagtc gagcactcca
    13261 cgcggcacca ctgcagcacc agaacggcga aagaagcaag aaaagcagtc gcagaacgtg
    13321 acgaaatggt gatgggatgc tggacgcatg cgtccaacgt ggtataaccc gccccgttac
    13381 gaacatcgcg agctgcttcg tgcgccgggc caccggacgg cgcgctgcgg tgtggtcgac
    13441 cgagcgggca cgccggcggt tggcatggac cgggtcgcgt ctgcgtcgag acgagattgg
    13501 caggaggagg cgcctcatgg ccgccaggat gatgcagttc accgacgtgc cgcaggcgat
    13561 gccgaacaag cgcgccgccg acgagcgggc gcaggatttc ggcgagatct acggccgcta
    13621 cgacgagcgc cgggccgccg accaggcgtc gcgttgcgag cagtgcggca ttcccttctg
    13681 ccaggtgcat tgcccgctcc acaacaacat cccggactgg ctccggctga ccgccgaagg
    13741 gcgcctcgag gaggcctacg agctcgcctc ggcaaccaac aacatgcccg agatctgtgg
    13801 ccggatctgc ccgcaggacc ggctctgcga gggcaattgc gtgatcgagc aggccggcca
    13861 cggcacggtc accatcggtg ccatcgagaa gcacatcacc gacaccgcct tcgacatggg
    13921 ctgggtccag cccgtgcggc cgctgcacga gcggccggag agcgtcggca tcatcggcgc
    13981 cgggccggcc gggctcgccg ccgccgagga gctgcgccgc gaaggctacc aggtgcgcgt
    14041 ctacgaccgc tacgaccggg tcggcggcct tttgatctac ggcatccccg gcttcaagct
    14101 ggagaagccc attgtcgagc gccgcgccga gctgctgcgc cagggggggg tcgccttcga
    14161 gctgggcgcc gatatcggcg gggccggtgc cgcgctcggc ttcgccgagc tgcgccggcg
    14221 gcacgatgcg gtgctgatcg cgaccggggt ctacaaggcc cgcgctttgg cagcgccggg
    14281 tgtcggccag gacggcgtgg tcgccgccct cgactacctc accgccagca accgcaaggg
    14341 cctcggcgac agcgtgccgg cgttcgacgc gggcacgctc gacgcccgtg gcaagcatgt
    14401 ggtcgtcgtg ggaggtggcg acaccgccat ggactgcgtg cgcaccgccg tccgccaggg
    14461 ggcgacgtcg gtgagctgcc tctatcgtcg cgaccgggcc aacatgccgg gctcggcgcg
    14521 cgaggtcggc catgccgagg aggagggggt cgagttcgtc tggctcgccg cgccggaggc
    14581 cttcctcggc gatggcaagg tgcgcgccgt gcgcgccttc cggatgcggc tgggtgcccc
    14641 tgacgccacc ggccggcaga cgccggagcc ggtcgagggc tcgaccttcg agcggccggc
    14701 ggatctcgtc atcgccgccc tcggcttcga ccccgaggat ctcgtgacgc tcttcgacga
    14761 gcccgagctg ccggtcagcc gctggggcac ggtcaagatc gactggcgga cgatgatgac
    14821 ggcgctgccc ggagtgttcg ccgccggcga catcgtccgt ggcgcctcgc tcgtcgtctg
    14881 gggggtgcgc gacggccgcg aggccgccgc cggcatccat cgccatctgc agtccagcga
    14941 gcgggccggc gccgacagcg ccgccgccct cgctctcgcc tcgtgaaggt cgccgccatg
    15001 tcgcaatcca gcatcgagca ggcgctcgcc acctaccagc gcgagcgggc gaagctcgag
    15061 gcgctcggct acgaggccga ggccttcgag cgcgacgcct gcggcgtcgg ctgcgtcgtc
    15121 gccatcgacg gccggccgcg gcgcgaggtg gtggtcgccg ggatcgacgc gctgaaggcc
    15181 gtctggcacc ggggtgcggt cgacgccgac ggcaagaccg gcgacggggc gggcatccac
    15241 atccagatcc cgcaggactt ctacaagggc cagatccgcg gcatgcgcga ggccgaccac
    15301 cacgtcgccg tcggcatggt gttcctgccc cgcaacaaca tccaccagca ggaggcctgc
    15361 cgcaccatcg tcgagagcga ggtgctgcgc ttcggctacg tcatccgcgg ctggcgccag
    15421 gtgccggtcg acaccagcgt gatcggcgag aaggcggcgg ccacccggcc cgagatcgag
    15481 cagatcatca tcggcaaccc gatcgacggc gacggcccga acgacagcga ggccttcgag
    15541 cgcgacctct atctgatccg ccggcgcatc gagcggctgg cgctggccga gagcctcgcc
    15601 gatttctaca tctgctcgct gagctgccgc tcgatcatct acaagggcct gttcctcgcc
    15661 gagcagctcg cgaccttcta ccccgacctg caggacgagc gcttcgtctc gtccttcgcc
    15721 gtcttccacc agcgctactc gaccaacacc tggccgtcct ggaagctggc ccagccgttc
    15781 cgcacgctcg cccacaacgg cgagatcaac acgctgcgcg gcaacgtcaa ctggatgaag
    15841 agccacgaga cgcggatggt cgccgacatc ttcggcgagc acaacgagga tctgaagccg
    15901 gtcatccagg ccggcagctc ggacagcgcc tcgctcgacg ccgtggccga ggcgctggtc
    15961 cgcgccggcc gctcgctgcc gctggtcaaa acgctcctgg taccgcccgc ctggtcgaac
    16021 aagatggcca tgccccaggc gcaccaggac ctcttcagct actgcaactg cgtcatggag
    16081 ccctgggacg ggccggcggc gctggtcgcc accgacagcc gctgggtgat cgccggcatg
    16141 gacaggaacg gcctgcggcc gctccgctac agccggacca gcgacggcct gctcgtggtc
    16201 gggtccgagg ccggcatggt gccgctcaag gaggtcgaca tcgtcgagaa ggggcgggtg
    16261 gggcccggcg agatgatcgg cgtcgacctc gtcaccggca ccttcttccg cgacgtcgcc
    16321 ctcaaggacc gtctcgcggc gcaccggccc tacggcaagt gggtcgagaa catccgcctc
    16381 ctcgaggatc ttgtggcggc gcggccgccg gcccccgtcc attacgggcg ggaggagctc
    16441 aggcgacgcc aggcgctctt cgggctgacc atcgaggaca tggagctgat cctctcgccc
    16501 atggtgctcg atgccaagga ggcggtcggt tcgatgggcg acgacacgcc cttggccgtg
    16561 ctctccaagg gctatcgcgg cctgcaccat ttcttccgcc agcagttcag ccaggtcacc
    16621 aacccgccga tcgacccctt gcgcgaatgg cgggtgatga gcctcaagac ccgcttcggc
    16681 aatctcggca acatcttcga cgaggacccg agccagacac ggatcctgca gctcgagacg
    16741 ccgatgctga cgaccgacga cttccgggcg ctcgaggcct atttcggcgc caatggccgg
    16801 accatcgact gcaccttcaa ggcgaagggc gagcggctgc gcgaggcgct ggcccgaatc
    16861 cgccaggagg ccgaggaggc ggtccggggc ggctgcgagc agctcatcct gtccgacgag
    16921 gcggtcggcc cggaacgggc gccggtgccg atgatcctcg ccaccagcgc cgtccacagc
    16981 cacctcgtcc gccaggggct ccgcgccttc agctcgctca ccgtgcgcac cggcgaatgc
    17041 ctcgacgtcc attacttcgc cgtgctgatc ggcgtgggtg cgaccacggt gaacgcctac
    17101 ctcgccgagg cggcgatcgc cgatcgccac gcccgcggcc tcttcccggg cctgacgctc
    17161 gccgagtgcc tcgaccgcta caaggaggcg gtcggccagg gcctgctcaa gatcatgtcc
    17221 aagatgggca tctcgatcat cagctcctat cgcggcggct gcaatttcga ggcggtcggc
    17281 ctctcgcgct cgctcgcccg cgagtacttc ccggggctga tcacccgcat ctccggcatg
    17341 ggcctgaccg ccatcaagaa caaggtgcgc gcccagcacg agcgcgccta tgccgaggcc
    17401 gagccggcgc tggccatcgg cggcttctac cgctaccggc gcagcggcga gcggcacgcc
    17461 tacgaggcgc gcatggtgca ccagctccag caggcggtgg cgaccgattc ctatgtcgcc
    17521 taccagcgct tcagcgacat ggtgcaggcc ggcgacccgg tctcgatccg cgacctgctc
    17581 gacttccgcc agggccgcac gccggtgccg ctcgacgagg tggagagcat caccgagatc
    17641 aggaagcgct tcgtcacccc cggcatgtcg ctgggtgcgc tcggacccga ggcgcacgag
    17701 acgctgacca tcgccatgaa ccggatcggg gcgaagtcgg attccggcga gggcggggaa
    17761 ggggtcgagc gctacacgcc gaggcccaat ggcgacaatg ccaactcgcc gatcaagcag
    17821 gtggcgtccg gccgcttcgg cgtcaccgcc gagtacctga acgccgcccg cgagctcgag
    17881 atcaaggtgg cgcagggggc gaagccgggc gagggcggcc agctcccggg cttcaaggtg
    17941 acggtcgaga tagcacggct gcgccacgcc acgccgggcg tcaccctgat cagcccgccg
    18001 ccccaccacg acatctactc gatcgaggac ctggcccagc tcatctacga cctcaagcag
    18061 atcaaccccg acgccaaggt ctgcgtcaag ctggtctcgg aggccggcat cggcaccatc
    18121 gccgcgggcg tggccaaggc caaggccgac gtcatcctga tctcgggcca tgtcggcggc
    18181 accggtgcga gcccgcagac ctcgatcaag tatgccggca gcccctggga gatgggcctt
    18241 tccgagacca accagctcct caccctcaac cgcctgcgcc accgcgtcac gcttcggacc
    18301 gacggcgggc tcaagaccgg tcgcgacatc gtcatcgccg ccatgctggg agccgaggag
    18361 ttcggcatcg gcacgctctc gctggtcgcc atgggctgca tcatggtccg ccagtgccac
    18421 tccaacacct gccccgtggg ggtttgcacc cagcgcgagg atcttcgcgc caagttcacc
    18481 ggcacgcccg agaaggtcgt gaacctgatg agcttcatcg ccgaggaggt gcgcgagatc
    18541 ctggcctcgc tcggcctgcg ctcgctcgag gaggcggtcg gccggagcga ccttttgaag
    18601 caggtgggcc gcggcagcga ggatctggac gatctcgacc tcaacccgat tctcgcccag
    18661 gttgacccgg gcggtctgcc gcgtcactgc acgctgaagg gtcgaaacga ggtgccggac
    18721 agcctcgacg cgcagatgct gaaggacgcg cgcgccctcc tcgaggacgg cgagaagatg
    18781 cagctccagt acaacgtgcg caacgtccag cgggccgtcg gaacgaggct ctcggcgcac
    18841 atcacccgcc gctacggcat gaaggggctg cagccgggcc acctcaccgt gcgcctcagg
    18901 ggctcggccg gccagtcgct cggcgcctgg gcggtgcagg gcctcaagct cgaggtcttc
    18961 ggcgacgcca acgactatgt cggcaagggc ctctcgggcg gcaccatcgt cgtccgcccg
    19021 accgtctcga gcccgctggt cgccgaccag aacttcatca tcggcaatac ctgcctctat
    19081 ggcgccacgg acggcaagct gttcgccgcc ggtcgcgccg gcgagcgctt cgccgtccgc
    19141 aactcgggtg cgatgaccgt ggtcgagggc tgcggcgaca atggctgcga gtacatgacc
    19201 ggcggcgtgg cggtgatcct gggcgccgtc ggcgacaatt tcgccgccgg catgtcgggc
    19261 ggcatggcct atgtctggga cgccgcgggc cagctcgagg cgcatctcaa ccccgacatg
    19321 gtgatcatgc agccggtcct ggtcgatcac tacgtgcagg agctcaagaa cctcctggtc
    19381 gagcatgtct ggcagaccca gagcactctc gccgagcgca tcctgcacga cttcgacatc
    19441 gagctgccgc gctttcgcca gatcgtgccg aaggagatcc tggacaagct gcccgtgccg
    19501 gtgcagccgc aggcggacgc ggaagcccgg ctcgcatgag gcgctttctg gttctggcgc
    19561 tggccctcgc tcttgcgggt ccggcactgg cgacgccgcc ggtgccggcc tcgcgcgacg
    19621 ccatcaacga agcgctggac ggcatccagt cgctgccctt gccactgccg gaggcgggcg
    19681 agctccgggt gggtcggccg gccgacgagc ggcggctcaa ccgcggcttc ctcgaggatc
    19741 tggcggccca gccggactgg ccggatcagg gcatcgtcat tgccgccggg cgctaccggc
    19801 tcgaggacgt ggcgaccgag ctcgatcgcc ccgagctgct cgcctgcgag accgatacct
    19861 gccgactggc ggcgccgctg gccgtcgagc acggcgccgt gctggtcatc gagggcctga
    19921 cggtcgagct cgagcagcgg gccggagcgg caatcgtcgc cttcggcgac ctcttcatca
    19981 gcctctccgc catcgaggga cgggccggcg acgcaccggc aaccacggat ggcgaggcgt
    20041 ttcgcccctt catcgtcgcc tttgaccgga gccggacggt catccgcggc agtcggctcg
    20101 ccgcgctcgg ctatgacggc aacggtgcga cgggcctctc cgtcaccgtc accagccgcg
    20161 acgacccgcc ggcccggccg gtgctgcagc tcgtcggcaa cctgatcgaa ggcatgtatg
    20221 acggcgtgca ggtgcgtggt gccgccgagg cgacgctcct gcgcaaccgg atcagggcct
    20281 cgctgcggca cggcatcgcg atccgcgacg gcaccgccga cggcctgatc gccgacaacg
    20341 acatcgtcgg cagcggtgcc atggccgaca acggcaacgg cctcctcctg tcccgcggca
    20401 tcacccggac ggcagtcctc gccaaccgga tcatcgacag cgccggggcc ggcatccaga
    20461 tcgacaaggg cgccttcgat ctgaccctcg ccggcaacga gctggcgcgc agcgggcgcg
    20521 acgggctcgt gctctacgag agctggtcgg tcgacctcca gcgcaacgcc atcgcccaga
    20581 acggccgttc cggcatccgc atccgcgccg ccgacggcat cctcgtctcc gagaacggcc
    20641 tcaccggcaa tgcccgcgcc ggcatcgacc tgcaggactg gagcacggtc acgaagccgc
    20701 cgagcgagga ggaggcggcg ctgatccgcc cgaccgaggt gaccgtcaag ggcaaccgct
    20761 tcgccgacaa cgccaggggc gactgcgtgg tgcagggcgt ggtcacgatc ctgccggcgg
    20821 aaggcagcga ttgctgagac gggcgacgct cgccaccctg gccttcctcg ccttcgcttc
    20881 gtccaccctg gcccaggtgc ccacgccggc cgagatcgcc gccctcgccg acgagatcct
    20941 cgccgagccg gtgccggccc cgctggccgg cgaggtcgcc ctggccgagg ccgcgaacga
    21001 gccggcgctg cggcggggct tcctcgacga gctcgaggcg ctgccgggct ggcagggcgt
    21061 cgccatcgtg ctgcgctccg gccggcatcg gctcgaggcg ctgccggccc ggatcgaccg
    21121 gcccgatctc ctcgcctgcg acggccggcg ctgccggctg gccgccccgc tcgtcgtcgc
    21181 cgccggtgcc gtcctcgtca tcgacggcat cgagctgcgc ctggcgcagg aaaccggcgc
    21241 cctggtcagc gcccatggcg ggctcttcat cagcgattcc gagctcctcg gctggaacga
    21301 tgaggccgac atgccggccc ggaccgacga ccgcggcacg gcgttccgcc cctggatcac
    21361 cgggctcgag gacaaccgga cggtggtccg ccgcagccgc ctcgcccatc tgggctacca
    21421 gggcacctcc accttcggtc tggccttcac ccatggcggc cgcgatccgg cggaaggggg
    21481 gccccgggtc agcgtcgtcg ccaacgccat cgaggacctc tatttcggct tcttcagctt
    21541 cggtgcagtg ggggtcgagg tcatccagaa cgagatcagc cggagccacg tctacggcgt
    21601 cgacccgcat gacgcgaccc gcgacatgct gattgcccag aacaccgtca ccggcacgcg
    21661 gcgcagccac ggcatcatcc tcagccgcga gatccatgat gcgatggtgg tcctgaacca
    21721 gagcagcgac aatgccggcg ccggcctctt catcgacaag ggcagccacg acgtgacctt
    21781 tgcccgcaac cgcgccttcg ccaatggcaa ggacgggatc gtcgtctacg agagccgtga
    21841 catcaccatc acccgcaacg agctcttcgc caatggccgc gccgggatcc gcgtgcgcgc
    21901 ctcggccgat gtccgggtgt cgggcaacgc catccggcac gatgccggcc ccgggatctt
    21961 cgtctacgac tggagccacg ccgcccgcga gcccgacgag gaggatgcga cgcacctcct
    22021 gccgacgagc gtcgagcttt ccagcaacag cctcgccaac aatgccggcg gcgactgcag
    22081 catccagggc gaggtcgaga tccgcatccc gcccaatggc tacagcgact gctgacggct
    22141 tcgtcaggcc tgcgcgaagc cggccttggt tggcaggccc atccgcttca acgtcatcac
    22201 cacgggacag acgccggtga agctcgcctg gatcagatgc gcgcccaaaa gcccggtgac
    22261 ccagagccag ttgaggcttt gccagacagc cagcaacacc gtgaccagca ccagaacgcc
    22321 aacgacgagg agaatggcac gttccaccgt cattccggtc ctcccgcgat tgtccaactc
    22381 ggcattatcg gcagtcggac agaggcgcgc aagtccgccg tcagtaatgc gccggcacca
    22441 gctcgccggc acccagcccc tcgcgcgcac ccttgggccg cgactgcgcc ttgggcatct
    22501 tcggcagctc cggcgggacc ttctcgtaag gaatgctgtc caggaggtgc ctgatcaggt
    22561 tgagccgggc gcggcgcttg tcgtcggccg ggacaatgtg ccagggggcc tccggcgcat
    22621 gcgtggccgc gatcatccgc tggtaggcgg cggtgtagtc ccaccagcgg cgcaccgatt
    22681 cggtgtccat cgggctcagc ttccagtgct tgagcggctc cttgatcctc gccccgaaac
    22741 gccgccgctg ctcgccctgg ctgacgtcga ggaagtactt gcgcagaatg atgccgctat
    22801 tgacgatcat ttcccgctcg aaggccgggg tcagggccag aaagcgctcg tattcctcgt
    22861 cggtgcagaa ccccatcacc cgctcgacgc ccgcccggtt gtaccacgag cggtcgaaca
    22921 ggatgatctc gccggccgcc gggaaatgct ggatgtagcg ctgggcgtag atctgggtcc
    22981 gctcgcgatc cgacggcgcc ggcagggcga catgcttgaa cacgcgcggg ctggcgcgac
    23041 cggtgatgcg actgatcacg ccacccttgc cggccgtgtc ccgcccctcg aagacgacga
    23101 tgatccgctg ctgcgccgcc ttgacccagg cctgcagccg ggtcagctcg acctgcagct
    23161 cgtgcagctc ctgctcgaag accttgcgct tcatcttctc gggcgcggct gcctccgccg
    23221 ccgggcttgc cttcgccgcg tgcttcttct tgcccaagac cctctccact cgatatccgg
    23281 tgtagtcgcg agtgtgaccg gggtcgggcg gccgggcaag gcgtgacgca ggctggcctc
    23341 gggctcacgc cggatcgtcg gcgatgctcg cctgttcctg aaccgctcgc tcgcgctccg
    23401 gccacgtgct cttgatgaag gcgaggacgg cctcgatctc ggcgtcggtc atgaggtcgg
    23461 cgaagcccgg catgtcgctc tcgtaggcac caccgacgat ggccgcaggg ccgtccctga
    23521 cgatgcggaa caggatggaa tccgcatggt gccaggtgtg gccggaggcg tcgtgcgggg
    23581 gagcggggag gcggccggaa gaaagtcggc gctgccagtc cggctggcct tcgagctcga
    23641 ctccgtggca ggaggcgcag tgctcgccat agagcgcggc gcccagggcg agcatctcgg
    23701 gcgaagcggc gcttctcgtg gccggctccc cgagcccgcg ccagagggct gcggcgccga
    23761 gggcggcggc aatcgccacc gccccggcca cgaggatgcc gcgtctcatt cgtcggcgta
    23821 gagctcgggc ccgccggcgc cgatggcgaa gacgcggaag ggctcgagct gctcgcccat
    23881 catgccgggc gagcccgccg gcatgccggg cagcgtgatg ccgcgcaccg ccggccgctc
    23941 gtcgagcatc ctctcgacca cctcgaccgg cacatggcca ctgacgaagt agccgtcgat
    24001 catggccagg tggcagccct ggaagtcgtc ggggatgccg gccagccggc tcagcccgac
    24061 gatgtcctcg actggccgcg tcgtcacctc gaagccgctg gcctcgagat agtcgccgta
    24121 ggcggcgcag cagccgcaac tggggctctt gatcagctcg acgctgcggc tgggctcctc
    24181 ggcatggcca tttgcggtcg agccgacggc caggccgagc acggccacga aaagaagatt
    24241 gcgcatgtga aaggctccgt tcggtggatg cgcccggtcg cgagggccgg ggtcgacggc
    24301 agcacctgac ggccctccgg ccgccgtcgg agggggctcg gggcaggcga ccgcccgttc
    24361 agctcgtgac ggggctcttc ggtggatgcg gctcgggcag accgggccgg ccccgcggca
    24421 gcgcgctccc gcttggccat ggcgacagac ggcctgaagc ccccgcgagg gcggcttcgc
    24481 ttgggatcgc cgtcagcggc ccggcgcaga catcggcgca ggccatcatc gcgtcctgcg
    24541 gcatggcgca gtcggggcag ccgccgtcca tcgccgaatc tacatggccg ctcggcaggc
    24601 ccgcgacgcc cgcggtcatg gcgacggctg cgacgtgcat catcgtgccc atgatgaaga
    24661 ggcacatgat gaccaaagac agagaccgga cgcccgcacg catgctcgaa cgttcaccga
    24721 ccgccacggg cttgtcaagg cgtcgccggg ttgcggcgcg ccgtcactcg ccccgctccg
    24781 gccgcgtctg caccgggtcg atcttgccgc ccggctcgaa gatcgccgcc cgcacatggc
    24841 tgaggcgcgg cggcggctcg gcgatgaagg ggagcggcct ggtcaggcgg atcgcctcca
    24901 gccccagccg gccggcgagc agcgcattgc ccgcgccctg gccggcccgg gccgagagca
    24961 gggccgcgag cgaggcgccg acatgctcga cggcggcgtg gctgaccacg tcggcgagcc
    25021 cggcgatggc ggcgttcctg atgcagcggc gcagcaggtt gaaggccgcc gccgagccga
    25081 gcgccagccc ataggcgcgg gcgatgccgc ggatcatcag cgtcgcccgc cagagcacga
    25141 gcaggccgtc catcaggccg agcgggctca gcgccgtgag caggccgata tcgcggctcg
    25201 cctccagcac cagccggtag gcgcgcttgt cccgaggggc gagcacctgc cgctcgtaga
    25261 ggctgagcac ctcgctgtcg tcgaggccgc gctcgctcgc ggccctgatc tccgccttgt
    25321 ggcgctcgcc gagctgctgg tcgagatcgt cgagatagcc caccgcctcg ccgtgcagct
    25381 cggagccggc gagccgctgg ccggtgagcc gcagccgctc ggcccgacgc acccggcgaa
    25441 ggtcgaagac ctccttccag agcgtgccga gcgccgtgac caccacgacc gcgagcagca
    25501 ccgcgaaggg ccagccgagc agcggcgtcg tctcgatcag cccggtcacg tagtcgatcg
    25561 cctggagcag cacgaggccg atgatggagc cgacgaggag caggccaggc cggcgccaga
    25621 aggggcgggc gggcggcacc ggcggcggcg ccagctcggc cggcagctcc ggcgtctcct
    25681 cgacggtctc gaaccgcccg gcccgggggt cgagctcgaa cggccggacc ggtggcagct
    25741 cacccttcat cgcagcttgt ccccaatgag gaactcgatc gcctggtcga gccggagatg
    25801 ctgccccttg accccgggct ccaggcgtct gggctcgaag tcgcgaaagc ggaagcggcc
    25861 cgagcgccag tcctcgtcgt ccggcaggtc gcccgggatt tcccccggaa agagcaccgt
    25921 ctcgcgccct tcgtcggcca gccggccgcg cacgcaggag agaagctggc cctgatgctc
    25981 ggtcctgacc gtgtcggtcg cacgcagcgc ggcgagcgcc agcacgtccg tctcgagccc
    26041 ggcgaagcgc gccttgcgcc gggccggggc gatcatcagc tcgagcagct gcttgaggtt
    26101 ggcgtgctgg ttggcggcca cgtggtcggc cttgctggcg gcgaagagca gcttctcgat
    26161 gctcggccgg aagagccggg cgagcaggct gttggtgccg tagcggaagc tctcgaggat
    26221 ggtctcgagc gcgtcgcggc tgtcggcgaa atgggcccgg ccacggttga gggtgtggaa
    26281 caggtcgacc agcacgatct gccggtcgaa gcggctgaaa tggtcggtgt agaagggctc
    26341 caccacctgc cgcttgtagg tctcgaagcg ggccgccatg gcgcgccgca gctcggcggg
    26401 tgcggcgtcg ggcaaaggtg cgaagccggt cgccgggtcg gccccggcgg tgaccagccg
    26461 gccgggctgg acgagactga ggccgagctc gttctgcacc cggccgagat agcgccggta
    26521 gacttcgacc agctccgccg gcgtcgtcgt cggcgagagc ccggccaggg ccgcccgcca
    26581 ctcgccggcg accgcggccc gcggctcgac ccggtcgcgc tgtgctgccg tctccgagaa
    26641 ggtcgggtag tccatctcca gcaagggcag gtcgagcagc cactcgcccg gatagtcgat
    26701 gatctcgagg atcaggctgc cgttcggcgc catcaggcgc tggacgaggc cggtcgtccg
    26761 aaagcgcagc tcgagcttga gcaccgtcag ccggtcggtc gcatcgggcc aggtcggacg
    26821 gtccgccatc agggtccggt ggaaggtggc atagggaaag gccggcccgc gcaccggcag
    26881 gagccgcgca ccccggtagc ggccctcgtg cacggcgccg acgaaggcga ggtcgtggcc
    26941 gtcgagcagg tgatgcacca gcgccgtcgt gaagaccgtc ttgccgctcc gccgcagccc
    27001 cgtcaccgcc agcctgagat gccggtcgac cagccggtcg ccgagctccg ccacctcgcc
    27061 cggcaggtcc cgaacgaagc cgccgagccg atcgagtccg agcatcagcg ccctacccga
    27121 cgctcatggc agctgccagt ggccgagcag cagcgccacg ccgacgagga gcccgaggcc
    27181 gcgatgactg cggaaccggt cgaggcagtc ggcgggatcg tcgaggtcga ccgcctgcac
    27241 ctgccaggcg agcagcccgg ccacgggcag gagggcgagg aagaagagcg ggccgagccc
    27301 ggcgctccag ccggcaagca cgagcaggcc gagcatcgcc gcatagaagc cgtagagcca
    27361 ggggcgcgtc gcctcgccga agagcagggc cgtcgatttc accccgatca gcgcgtcgtc
    27421 ctccttgtcc tggtgcgcgt agatggtgtc gtagccgagc gtccaggcga tgccggcgag
    27481 atagaggaag agggccggca ggccgagccc gcccatgctc gccgcgtagc cgaccagcac
    27541 gccccagttg aaggtgagcc ccaagaagac ctgtggccag taggtgatcc gcttcatgaa
    27601 gggatagccg atgaccaggg gcatgctgca gagcgctatc agcgctgcgg tcagcggcag
    27661 gaagaggagc acgccgaggc cgatggcgag ctgcagggcg aggaaggcca tggcctgctt
    27721 catgcgcagt cgcccggcgg cgagcggccg gccggcggtg cgcgccacgc ggcggtcgaa
    27781 gtcgcgatcc gccatgtcgt tgatcgtgca gcctgccccg cgcatggcga tggcgccgat
    27841 ggccatgagc ggcgcccaga gcatggccgg cgtgccgccg gccagggcga ggccccagag
    27901 gcacggaatg aacagcagcc acgtgccgat cggccggtcg agccgcgcca ggaggccgta
    27961 gtcgcgccag ccccggggca gccggaccag ccagccatat tcctcgtcag gcaatcggac
    28021 gacgggctcg ccagcttgtc cactcatgcc ggatcactta gcgttcgcct ccctcacggt
    28081 aaaggcggcg cccagcaatg cgcgacacgc gactccatct cgaggcaccg ctcggccccg
    28141 gcgccagcct cgatctcgag cccgaggcgg cccactacct gcgggcggtg ctccgcgctc
    28201 gcgccggccg gaccttgcgg gtcttcaacg ccggctccgg cgaatggcgg gcgcgcctcg
    28261 tccggctcga ccggcaccgg gcgaccctgg ccgtcgagac gcagctccgc ccgccggccg
    28321 ccgagcccgg cccgaccctt ctcttcgcgc tcctgaagaa gccgcggctc gagtggctcc
    28381 tggagaaggc ggtggagctc ggcgcggccc ggctccagcc ggtgcgctgc cggcacgccg
    28441 tggtcgaggc ggccaggccc gaccggctgc gccagcgcct gatcgaggcg gcggagcaat
    28501 gcgagcggct gagcctgccg gagctgctgc cggagacggg tctcctcgag gccgtgcacg
    28561 cggccagcga gcccggcgtc atcctcgccg acgaggcggg cggcgccgcc ctcgccccca
    28621 ccctccgccg atatcccgag gcggcattgc tggtcggccc ggagggcggc ttcgccaccg
    28681 acgagcggac ggcactccgc gccctgacgt tcgtccggcc ggtcagcctc ggcgccacca
    28741 tcctgcgggc cgagaccgcg tcgctgatgc tcctcgcctg tcaccgggcg ctgacctcgg
    28801 ccgcggatcg gccgtgccgt gccgaagctt aacagcgtgt tgcggaattg cgagtttcag
    28861 cgaccctcga gtccgatcac ga
//
LOCUS       DAOTFI010000021        28341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1902_length_28341_cov_6.902779, whole genome shotgun sequence.
ACCESSION   DAOTFI010000021 DAOTFI010000000
VERSION     DAOTFI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 28341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28341
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1902_length_28341_cov_6.902779"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="P5558_03365"
     CDS             complement(<1..1274)
                     /locus_tag="P5558_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY23382.1"
                     /translation="MGLTVLIGVFVLGLVIGVPVAVTLGLASAAYLLLEGIPLVSIPQ
                     KMYAGMDVFVLLCIPGFILAGNLMNSGGITERIIRFANALVGWMRGGLGLTNVAGSML
                     FGGISGTAVADAASIGGMMIPGMKKAGYPADFSAAITAASSTVGPIIPPSVPMIIVGA
                     LSGISVGKMFLAGAVPGILMGLAMMVTCYIIAVRRDFPRQAWAGFGELARSFMGAFWA
                     IAMTALIIGGLLTGLATPTETAIVASVYALAVGLLIYRELPIRLVPRIFIDSAISSAA
                     ILALVGFANVFGWILVSERIPQMIADAVLSVTDNKYLVILLINLLLLFVGMFMETIAA
                     LIILFVPLLALAQGVGIDPLHFATFAVLNLMIGLTTPPVGVCLFICANIAKLPLTPVI
                     VAITPFLLTNILVLLLVSYVPPIATWLPSVLMP"
     gene            complement(1277..1780)
                     /locus_tag="P5558_03370"
     CDS             complement(1277..1780)
                     /locus_tag="P5558_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY23383.1"
                     /translation="MRDKGSMGIVMGVVRAAVGIAFLVLCVAVLIQVLGRSVFNSSPV
                     WTEELTRFALLYLAAFGAGLSFRSGDLVNVDLVCEALPGRLPWLLRLVSALATAVLCA
                     ILLWPAWRYTSIGVMQTSPALGWRMDFIHASILVLLLSLLVFAALRVLRMLSGASDGR
                     PVQAEES"
     gene            complement(1868..2845)
                     /locus_tag="P5558_03375"
     CDS             complement(1868..2845)
                     /locus_tag="P5558_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY23384.1"
                     /translation="MQLAGTRRAALLGAMLGVLMANTSLAAEVTLKLGHLANEDNIWH
                     KAALKFGEEVATLTDGRIEVQVFPNESLGKEMDLINGMQLGTADMTITGESLQNWAPM
                     AALLAVPYAYSSLEDMDSVAGGEIGDQIEAQIVEKAQIRPIAYFARGPRNLTSNRPIK
                     SPDDLNNLKMRVPNVPLFVDVWSALGAQPTPMAFSEVFTSLQNGTIEAQENPLALIKS
                     ASFAEVQDYVNLTEHVRSWIYLTISEMAFGNLSAEDQEAVLEAGRRTQAYERELFLAD
                     EKQLVADLEAAGMEFVEVDHAAFAAKAKDAVLANVNADIQPVVEDLFAN"
     gene            3040..4257
                     /gene="uxuA"
                     /locus_tag="P5558_03380"
     CDS             3040..4257
                     /gene="uxuA"
                     /locus_tag="P5558_03380"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRY23385.1"
                     /translation="MRQTWRWFGPKDLVSIDDMLQAGVEGVVSALHHVPTGAVWSPAE
                     IEQRQAEIGRRRDGTPSGLAWEVVESLPVSEDVKKQKGEWRAHLANYGESLRNLAAAG
                     IEVVCYNFMPVLDWTRTDLTYKVAHGGTCMRFDLVDFAAFDLFILERRGAAEDYPETV
                     REAAAERFAGMDDGQKEQLTGNIAFGLPGAAETMTLDDLRRHLAEYGAISAETLRSHF
                     TAFLEAVVPVAEEVGIRLCCHPDDPPFPLLGLPRVMSTEADYAAIMQAVDSPANGITL
                     CSGSLGARPDNDLPGMMERLGDRVHFLHLRNVYRESDQVQGSFYEDEHLAGATDMVAL
                     LAAVIAEERRRKAAGRRDHTIPMRPDHGQDILDDLGRRAQPGYPSIGRLKGLAELRGV
                     MTALSHPRWGMAS"
     gene            4254..5735
                     /locus_tag="P5558_03385"
     CDS             4254..5735
                     /locus_tag="P5558_03385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HRY23386.1"
                     /translation="MTSRLSLASLGSIPATVRRPGFDPAGAGHGIVHLGVGAFHRAHQ
                     AVCTDDALAAAGGDWRIQGVSPRSTDIADALNPQDGLYTLLIRGPEGTEARIVGSLGR
                     VIAAAREPGAALAAMSDPATRIVSLTVTEKAYGLDRAGGGVLQGHPAIAPDLQSPRQP
                     KGAIGLLVEALRRRRQQGLDPFTVLCCDNLPENGTLLRAGVLDFAGRTDPALARWIEA
                     RVAFPSTMVDRITPAATPATLADAARLTGCEDQAAIETEPFSQWVIEDRFTAGRPAWE
                     AGGAIFVDDVAAYERMKLRMLNGTHSMLAYAGFLAGHRYVRDVMGDPVLARLVRRQLA
                     AATGTLPPLAGIDLGDYAAALCQRFANPAIGHETRQIATDGTEKLPQRLLAPALELSR
                     RGGDLRPFAFAVAAWMRYALGRTDAGDAYALRDPREAEIRAAVAGGGTPATIARALHG
                     LKGLFPPELVADPAWTSAVESILAGMLGRGVATALAEEAARTA"
     gene            complement(5746..6453)
                     /locus_tag="P5558_03390"
     CDS             complement(5746..6453)
                     /locus_tag="P5558_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY23387.1"
                     /translation="MTMAFPDEAVAAVANFRQGSMSSRVHDLLREAIIQLRLRPGNLL
                     SETELARQFGVSRQPVREAFIKLAEAGLVEIRPQRGTFVQLISRREVENARFIREAIE
                     AAFARKAAEAAAPGTTALLRQIIDRQEAARQADDTVEFLRQDEELHRAIAASIGCESA
                     WKLLETLRAQMDRVRFLSFPAATPIDVMIDQHRTITDAIEAGDPDAAERAMRTHLGEL
                     LKSLPRIAEAHPDLFVD"
     gene            complement(6548..7444)
                     /gene="yghX"
                     /locus_tag="P5558_03395"
     CDS             complement(6548..7444)
                     /gene="yghX"
                     /locus_tag="P5558_03395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HRY23388.1"
                     /translation="MTSPQRKSARDFDQRILEFYDGYVHGRMSKREFIARAGKYTAVG
                     VTGAMVLDELQPNYALAQQVAPDDPAIVTERVEYESPEGHGTMAGLMARPADASGPLP
                     AVLVVHENRGLNPYIEDVARRLAKEGYLAFAPDGLTPLGGYPGNDEEGREMQQRLDGA
                     KLMADFFAAFEHLRDHEASTGKVGAVGFCYGGGVCNALAVAYPDLAASVPFYGRQPAV
                     EDVPAIEAPLLLHYAGLDERINAGWEAYGAALEANGKEFTVHFYPDVNHGFHNDTTPR
                     YDEAAATLAWERTLAFFDTHLR"
     gene            complement(7712..7788)
                     /locus_tag="P5558_03400"
     tRNA            complement(7712..7788)
                     /locus_tag="P5558_03400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7752..7754),aa:Arg,seq:tct)
     gene            complement(7840..8127)
                     /locus_tag="P5558_03405"
     CDS             complement(7840..8127)
                     /locus_tag="P5558_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HRY23389.1"
                     /translation="MQARIYRPAKTAVQSGRRKTHEWLVEFEPRSRVEADRLIGWNGS
                     DDTAQQVRLRFPTKDAAIAYCRRENLTFQLFEPHVRVVQPKSYAENFIRRT"
     gene            complement(8167..8844)
                     /locus_tag="P5558_03410"
     CDS             complement(8167..8844)
                     /locus_tag="P5558_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRY23390.1"
                     /translation="MTDRAESAAAALADDLPLADEGREVRIVASRDAWRGFFRFEVVD
                     LKHRRFDGQMSPTITREILHIPEAAAVLPYDPVIDSIVLIEQFRAGCMRHPTGAWLIE
                     VVAGLVGPGECAVETARRELVEEAGLEARRIEKIATYVASPGAVTERSTCFIAEVDAS
                     GAGGLHGLEHEAEDIRSFVVPLQTAFDWLENGRILAANTIIPLRWLQAHHTELRRRWL
                     AESEKIA"
     gene            complement(8841..9083)
                     /locus_tag="P5558_03415"
     CDS             complement(8841..9083)
                     /locus_tag="P5558_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRY23391.1"
                     /translation="MQTIFVMVKCELGCSYEVAGKAVETIEEVSEVYSVSGQYDLIMK
                     FHLAADVDIGRFVNKAVQTLPGVKDTFTLITFKAFA"
     gene            complement(9118..10848)
                     /locus_tag="P5558_03420"
     CDS             complement(9118..10848)
                     /locus_tag="P5558_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY23392.1"
                     /translation="MTTEQATITFAELGLREEIQRAIADAGYVHPTPIQAAAIPAVLD
                     GRDVVGIAQTGTGKTAGFTLPMLHRLAAGRARARMPRSLILSPTRELATQISQSFETY
                     GKHLNLTMALLIGGVGMGEQEKLLEKGVDVLIATPGRLLDWFDRGRILLNGVEVFVID
                     EADRMLDMGFIPDVERIFQLLTKRGQTLLFSATMPKEVRRLAERFLRDPAEIEVARPS
                     TRAEKVIDVVVPIRGIERRVALDRLLEVEKIENAIIFCNRKRDVSSVNKFLQRQGRNA
                     QDIHGDLDQSQRQATLDAFKAGTVDFLVATDVAARGLDIVDLPVVINLDVPIHPDDYV
                     HRIGRTGRAGKEGHAYTLADPDDAKYLAAIERLLGSKLERKLIESLSDAAEAPRRPSR
                     ARPARRAPEREAEAVSAEAAPAPAAAAESDAAPAPRRRKRRSAPEAAPEASAAAVPSP
                     TEAAATEAGPIEAELTEAAPAEPAGTRRRRRRPETAEVAAEPVAVEATAETPAAGDTP
                     EAEAEPAKSRPRRRKAAADAPPTGVAKAAAPASKPERGRGEPRDEPVVGLGDHVPAFL
                     LRPVPIKAVA"
     gene            11011..11595
                     /locus_tag="P5558_03425"
     CDS             11011..11595
                     /locus_tag="P5558_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRY23393.1"
                     /translation="MMLSRSESLLLIVDVQAGLARAVHDAGSRITRLALLLRAAAVLD
                     VPVIVSEQYPKGLGATDPRLLPLPDGATVLPKLAFSCWREPVLRAALERAGRRQVVVA
                     GMETHVCVLQTALDLQEAGFAVHLAADASGSRRVEDRALGQARLRDRGVAVVTAEMVV
                     FEWLERAEGPAFKTLIPAIKALGTTDPGAARGRD"
     gene            complement(11602..12531)
                     /locus_tag="P5558_03430"
     CDS             complement(11602..12531)
                     /locus_tag="P5558_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRY23394.1"
                     /translation="MLERLDPAALVRLAAGASLTVAVLLTLGKLAAALASDSLAVLAS
                     MIDSLADIGGSLITFLAVRVSQQPPDRSHRFGHGKAESLSALAQASLVMGSALFVLIG
                     AAERLASPRVIEPGLFAFIVLVGAILITLALVTLQRVVIRRTGSTAIAADRLHYTADL
                     ATNLLVLAALALTAGLDVAWIDPVAAVLVALYLAWQAARLGRSAIDTLMDRELSGAER
                     RRIEALVRDHPDVVDLHDLRTRRAGNTTFIELHVEIDGAMSVRRAHRVTDALEARLAE
                     AFGETEIIIHQEPAGLEDDRLDHRIAEARAGSA"
     gene            complement(12541..13155)
                     /locus_tag="P5558_03435"
     CDS             complement(12541..13155)
                     /locus_tag="P5558_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRY23395.1"
                     /translation="MTMNLTDRDALRAHYGSVSRTASGKEMDRIDRHGRRFIELSPLV
                     VLATADAEGRCDASPKGDAPGFVKVLDERTLLIPDRPGNNRVDSLQNIVANPRVGLIF
                     FLPGYKETFRANGTARISTEPELLEATAHAGKAPRSVIVVSVEEAFMHCGKALIRSKL
                     WDPTQHIAKGQFPSLARILADQIEGIEEKATDALIEDAYERQLY"
     gene            complement(13248..16481)
                     /locus_tag="P5558_03440"
     CDS             complement(13248..16481)
                     /locus_tag="P5558_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY23396.1"
                     /translation="MASWLRELGLGQYVEAFRANDVDADLLASLTAEDLREIGVTSVG
                     HRRRLLAAIAHLGRRTDRSPAAEAPAPGPAAERRQLTVMFVDLVGSTALSARLDPEEM
                     GGILRAYQSAVAGEVAAFEGHVAKFMGDGVLAYFGWPRAHEDEAERAVRAGLAVIEAV
                     AGLAGHDPPLACRVGIATGLVVVGEPIGDGSASEQTVVGDTPNLAARLQALAGPGQVL
                     VARSTRRLLGAGFDVRELGDRALKGIDGPVPVFAVNGERVVASRFEARAGHLQPMVGR
                     EREFALLLERWRRARDGAGQAVLLVGEAGHGKSRIVHALGDALAGESYTLVRLQGSPY
                     HAGSALWPVIQQLRDEAGFADGQPNDVLLDRLEALPVGFPEQRDALPLIAELLGLDGA
                     ARYGRLELTPQARRARTREALADRLLGLARSGPLLVVVEDAHWLDPTTRELIATVLAG
                     LGRLRLLVLITSRPEGLPEWGTAAPLERLALDRLGRAGVEAIIRRLGGDGLPAGTIET
                     IVGRTDGVPLFVEELTKAVLESDDAAVPASLHDSLMARLDRLPEAKELAQVAACIGRE
                     LDQGLLAAVAGRPAAEVERAIDRLVRAELVFRQGPPAEGRLVFKHALVRDAAYESLLR
                     SRRREVHGRIARVLADKAAGHGGVEPQLVARHFLAADEPAAAMPHLLPAIDAAIARAA
                     APEARELIETGLAAVGRTPADETATGWRAKLHLLMGDSVRTTLGTAAPATAAAYRGSR
                     LAYAEIGDQDGQEMALFGEFLSHFNAARLDRADEVAGLLRAAPSQGAGPAGLWHRHEA
                     AGILDFVRGRFAAARRHLEALPEGEGPYPDRPTVGRIYLPWSLLVLGYPDEAARQEAA
                     HLQVAAGMGRKFVLAAALGNGCYIPCLTGRMGLLSERASRCLELCRNAHLTIYENIAS
                     VFVGWLAGKGGDAGRGDAMMAAALAALDSAAHHIELAYLSSLRADVLQQAGRIAEARA
                     LLEQGLARNAATGEGWFDAELHRLLAGCADDAGTAGRHLDAALEISRGQEAKLWELRA
                     ARDLARLRAGQGRRQEALDLLAPVLGWFTEGFALPDLVESRKLLETL"
     gene            complement(16572..17504)
                     /locus_tag="P5558_03445"
     CDS             complement(16572..17504)
                     /locus_tag="P5558_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY23397.1"
                     /translation="MVQGRLTAGLLLVACAGLLLAACAGLRERFEAPAAGEAAPPEVA
                     APAPAPAAPPAAVPPAASPGASAPRVTNVPALLRAAEAGDPKAQFDLADRYLDGRGVV
                     ADDEAAARWFLTAAEAGHAPAQYNMSLLYLMGRGVQPDEAAALRWLRAAAVQGLPEAQ
                     NRLGGFYAQGVAVARNDEEAARWFRLAAMQGDPPAQHNLGGMYYTGLGVPQDDRQAAH
                     WFRMAAAQGHRPAQFYLGTLYAEGQGVEPDPVEALKWVTLAATPPADLTIAARASKFR
                     AELMERMRPEQIAEAERLAAAWRPTPVAVPPRGS"
     gene            complement(17620..17919)
                     /locus_tag="P5558_03450"
     CDS             complement(17620..17919)
                     /locus_tag="P5558_03450"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRY23398.1"
                     /translation="MHDADAFLAISAMAVLTYATRAGGFLLMSMVPLSPRLEAFLRHL
                     SGSVLIAIIVPAAVTSGAAAILAILAAVLVMRRTRNALLAMAVGIGLAALLRAWV"
     gene            complement(17906..18601)
                     /locus_tag="P5558_03455"
     CDS             complement(17906..18601)
                     /locus_tag="P5558_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRY23399.1"
                     /translation="MERAVITWRGMGRGARELVAVGAYAFVFGVAFGIAARNVGMEGW
                     AANLMSAAVFAGGSQFAVLELWQSPVAWGPLLIATLAVNGRHFLLGASLYPWLRSLPA
                     WQVYLAAVLLSEPNWAKAIEAEARGERDVGVLIGGGVLLWVAWVAGTIAGSGFIDLSP
                     EHFRRFGLDLVFLAFLSCMLTGLRRGRSDDLAWLAAGITALAAMAILPAGWHILAGAV
                     AGGLVGVVVDARR"
     gene            18741..19853
                     /locus_tag="P5558_03460"
     CDS             18741..19853
                     /locus_tag="P5558_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY23400.1"
                     /translation="MTQNLKRRTMLAGTAAIGASATTAASFPRPAISQGVTRWKMVTA
                     WPKNLPGPGVAAQMLADRISALSGGRLEVELFATGEIVPGNQVFDAVAEGTAELYHAV
                     PSYWGSKSRGILLFGSQPFGLLATEQVGWLTHGGGQALYDEIYGRFGLKPFLCGNSGP
                     QWAGWFRNEITSVDDLQGLRFRTTGLASEMCSKLGMAVQAMGGGDMFQALQSGTIDAG
                     EFIGPWTDSALGFYQVAKNYYWPGVGEPSSAEECVVNMAAWEALPDDLKLAVETACKS
                     LYNDVLTEYSTKSAQSLPQLVAEHGVMVRQLPEEVMIAMGNAAGEVMAELGDDQDELV
                     RRIVESFLAYRASIASYMVYADNGQMNARALPFTYG"
     gene            19878..20387
                     /locus_tag="P5558_03465"
     CDS             19878..20387
                     /locus_tag="P5558_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRY23401.1"
                     /translation="MLATAADVLDRVNRAVGHAARWLALLMLLAQFLVVVLRYLFGTS
                     YIWGTETVLYLHAALFMLGAGYTLLVDGHVRVDILYAGLGERGKALVDLLGTLLFLLP
                     TCLVILWFTWRFTANSWAIREGPLSVGGIPASFLLKTLIPAFALLLLIQGISLALRSL
                     VRLQGGDSR"
     gene            20411..21943
                     /locus_tag="P5558_03470"
     CDS             20411..21943
                     /locus_tag="P5558_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY23402.1"
                     /translation="MLAALCLVILTGYPVTFLIAGLGVLFAFLGWAMGVFDLGLLGAL
                     AQRIFGVMTNQVLIAIPLFVLMGTILERSRIAEELLEQMGRLFGTIRGGLAVSVTLVG
                     ALLAASTGIVGATVVTMGLIALPAMLRNGYGRGFAAGSVVTAGTLGQIIPPSTMLIIL
                     ADVMSTAFQQAQYTQGKFSVETISVGQVFAAAILPGLLLVGLYIAYILWTAKRRPDLA
                     PAMPATDEKPSPAAVLKALVPPIVLIVAVLGSILGGIATPTEAASVGAIGALLLAGLR
                     LAPDRSGWPVWLAIAAIVALLVLTSFVDLRLGRSLSTTTESLAIWLAFGLVALLAIGL
                     VQCLVRAQRTGILVPVLTQTMTITSMIFGTIIGASIFSLVLRGLGGDARIEGLIEALP
                     GGPGLALLFVMAAVFLLGFFLDFVEISIIILPLTVPILLLMGFDPIWLAVLIAINLQT
                     SFLTPPFGFSLFYLRGAAPPEITTGDIYKGVIPFILLQILAILVVYLAPPIATVLPGL
                     LF"
     gene            22014..22292
                     /locus_tag="P5558_03475"
     CDS             22014..22292
                     /locus_tag="P5558_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY23403.1"
                     /translation="MTLVKLRKSAQLTLPTEIREALKLAEGDYLEAEIVPGGVLLRPV
                     DVVRREAAWDDLVELVETPKWRTPPTESPEEEEALIFKMVEEARHSRD"
     gene            22294..22731
                     /locus_tag="P5558_03480"
     CDS             22294..22731
                     /locus_tag="P5558_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRY23404.1"
                     /translation="MVLDSSVLVSAFLTPRGTSATVLVATRQARFTLCLSREIVTETT
                     TRLIRKLPRLAPKYAYTEADVARFARDLAETAAFVTDLPELDAVPLDPDDDIIIATAV
                     AARADLLVTGDRHLLDLGTHDAIRIVDPRRFLDELAKARPGAG"
     gene            22800..23459
                     /locus_tag="P5558_03485"
     CDS             22800..23459
                     /locus_tag="P5558_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY23405.1"
                     /translation="MTLTLYGVARSRASRNIWLCLEADLPFEQVPVIQARRVKDPLAP
                     DAPLNTRSPAFLAINPNGLVPAMRDGELVLFESLAINLYLARKTGAPLGPKDLAEEGR
                     ILMWSLWSATEAEPWAIQILMHRAYLPEPERDPAAATRAAQALVPKFAVLDQHLKGRD
                     WLVADRFTVADINTAEVLRYAALATELFDQAPNVKRWFKACQARPAFKEMMRRREAEP
                     A"
     gene            23604..24635
                     /locus_tag="P5558_03490"
     CDS             23604..24635
                     /locus_tag="P5558_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRY23406.1"
                     /translation="MARSKTVNRQWVLAERPKGEPDDRTLRLVTGDVPTPGKGQMLLR
                     NEYLSLDPYMRGRMSDRPSYAPPVELGAVMVGGTVAEVLGSDLDAFAPGDRVVASGGW
                     QDYALSDGTGVINLGPNPKNPSWALGVLGMPGLTAWAGLTEIGRPKAGETLVVAAASG
                     PVGATVGQVGKILGCRVVGIAGGAKKCAHVVDALGFDACIDHRADGFADALKAAVPDG
                     IDIYFENVGGAVLDAVLPLLNPKARIPVCGLVSIYNATSLPDGPDRLGQLMMMILRKR
                     MTLQGFIVFSDFGHLYPKFASQMGAWVEAGRVRYLEEIIEGLEQAPAALIGLLKGESF
                     GKRVIRLAS"
     gene            complement(24800..25306)
                     /locus_tag="P5558_03495"
     CDS             complement(24800..25306)
                     /locus_tag="P5558_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRY23407.1"
                     /translation="MAKEKTLDDLFHDTLRDIYYAERKILKALPKMKRAAQSDDLKAA
                     FDKHKQETEGHVERLQQVFDIIGKRAQGKTCDAIEGIIAEGEEIIEEFKDTAALDAGL
                     ISAAQAVEHYEIARYGTLKRWARELGMDDAAKLLDATLQEESRTDSDLTKLADASAND
                     RAKARKAA"
     gene            complement(25555..26244)
                     /locus_tag="P5558_03500"
     CDS             complement(25555..26244)
                     /locus_tag="P5558_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23408.1"
                     /translation="MMAPSGAAEVRMGYVATERVACAGTPVNGELRGLRMVPLGAALW
                     TGAGRLRAQGIRGIAHAAPGSMTLQGRPGFDPTRASVEASIRNAFTLAAAHGCRRLAL
                     PFIAGGVFAGRIDPAIEKAELAALIVAACHTHRGEVGAVIVAHGDEDRALFATALAAG
                     SVRGTTLVQGSITRVADHGCDAIANAANMEVRFGGGISGVIGRATGESDEIDREAAAA
                     IGQFWGRNGPG"
     gene            complement(26644..27669)
                     /locus_tag="P5558_03505"
     CDS             complement(26644..27669)
                     /locus_tag="P5558_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY23409.1"
                     /translation="MTRRTIGLIGLGMAVAPHAQSLEDLQGRVEVRAWSPSAERRRAF
                     EERYDLPVVDELEAIADDPAVTAVMILTPPDARLELVGQLAGKGKHLLMEKPLERTLE
                     AAERVVALTEAAGVTTGVVLQHRFREAATELARRLADGELGTLATVQLSVPWWRPQSY
                     YDVPGRGTLARDGGGVLITQAIHSLDLMLSLTGPAAAVQAMGGTSAMHRMEGEDFMGG
                     GIAFANGALGAVMATTTFHPGRAEQLVLAGTKGVAVLDGGRLTLDFLDGRSETTGEAG
                     GIGGGADPMAFSHEWHKAVITDFLDALDQGSAPRVTARDALHVHRLIDALLRSAEEGR
                     RIEIAAL"
     gene            complement(27666..>28341)
                     /locus_tag="P5558_03510"
     CDS             complement(27666..>28341)
                     /locus_tag="P5558_03510"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY23410.1"
                     /translation="AIEEAVERTNLARYLPPEIAALLAAGDVEALRRGWDVRIGILVV
                     DIRGFTSRSEHLAPPELSTFLGTFRHHVVKAALTHRGVVDKFVGDSAIVVFGVPEGHA
                     DDARRTLDCARHLLRRVAAWSDRLTAAGEAPVRIGIGAHFGPAFAGAVGDSSRLEFTV
                     LGDTVNVAARLEQITRETDGGLVASAALVAAAGEGDHWLALPTHAVRGREQPIDILHW
                     QGEQET"
BASE COUNT         4138 a        10252 c         9789 g         4162 t
ORIGIN      
        1 ggcatgagga cggagggcag ccaggtcgcg atcggcggca cgtaggagac cagcaggagc
       61 accaggatgt tggtcagcag gaacggggtg atggcgacga tgacgggggt cagagggagc
      121 ttggcgatgt tggcgcagat gaagaggcag acacccacag gcggcgtggt caggccgatc
      181 atcaggttga gcacggcgaa ggtggcgaag tgcagggggt cgatgccgac cccctgggcc
      241 agcgccagga gcgggacgaa gaggatgatc agggccgcga tcgtctccat gaacatgccg
      301 acgaagagca gcagcaggtt gatcaggaga atcaccaggt acttgttgtc ggtgaccgag
      361 agcacggcgt cggcgatcat ctgcgggatg cgctcggaga cgaggatcca gccgaagaca
      421 ttggcgaagc cgacgagggc gaggatcgcc gccgaggaga tggcgctgtc gatgaagatc
      481 ctgggcacga gcctgatcgg cagctcgcgg tagatcagga ggccgaccgc gagggcgtag
      541 acgctggcga cgatggcggt ctcggtgggc gtggcgaggc cggtgagcag gccgccgatg
      601 atcagggcgg tcatggcgat tgcccagaag gcgcccatga acgaccgggc gagctcgccg
      661 aagccggccc aggcctggcg cgggaaatcg cgccgcacgg cgatgatgta gcaggtcacc
      721 atcatggcga ggcccatgag gatgcccggc acggcgcctg cgaggaacat cttgccgacc
      781 gagatgccgg agagggcgcc tacgatgatc atcggcaccg agggcgggat gatcggcccg
      841 acggtggacg atgcggcagt gatggcggct gagaaatcag caggatagcc ggccttcttc
      901 atgccgggta tcatcatccc gccgatcgag gcggcatcgg ccacggcggt gcccgagatg
      961 ccgccgaaga gcatcgagcc cgcgacattg gtcagcccca ggccgccgcg catccagccg
     1021 accagcgcgt tggcgaagcg gatgatgcgc tcggtgatcc cgcccgagtt catgaggttg
     1081 ccggcgagga tgaagcccgg gatgcagagc aggacgaaga cgtccatgcc ggcatacatc
     1141 ttctgcggga tcgagacgag cgggatgccc tcgagcagga gataggcggc cgaggcgagg
     1201 ccgagcgtta cggccaccgg cacgccgatg accaggccca ggacgaagac gccgatcagg
     1261 acggtgagtc ccatggtcag ctctcctccg cctggacggg ccggccatcg ctggcgccgg
     1321 acagcatgcg gagcacgcga agggcggcga agacgaggag cgacaggagc aggaccagga
     1381 tgctggcgtg gatgaagtcc atccgccagc cgagcgccgg cgaggtctgc atgacgccga
     1441 tcgaggtgta gcgccaggcc ggccagagca ggatggcgca gagcaccgcc gtcgccagcg
     1501 ccgagaccag gcgcaggagc cagggcagcc ggccgggcag cgcctcgcag accaggtcga
     1561 cattgacgag gtcgcccgag cggaaggaca gccccgcgcc gaaggcggcg aggtagagca
     1621 gcgcgaagcg ggtcagctcc tcggtccaga ccggcgagga attgaacacc gagcgaccca
     1681 gcacctggat cagcacggcg acgcagagca ccagaaaggc gatgccgacc gctgcccgca
     1741 ccacgcccat gacgataccc atcgagccct tgtcacgcat ggggtccccc atccatcgac
     1801 accacctgaa acggtcgagg tcggcggcgc cacgggctgc gtggcgccgc gcctgtcgag
     1861 cggacggtca gttggcgaag aggtcttcga ccaccggctg aatgtccgcg ttgacattgg
     1921 cgagcacggc atccttggcc ttggcggcga aggcggcgtg atccacctcg acgaactcca
     1981 tgccggccgc ctcgaggtcg gcgacgagct gcttctcgtc ggcgaggaag agctcgcgct
     2041 cgtaggcctg cgtgcgccgg ccggcctcga gcaccgcctc ctggtcctcg gccgagaggt
     2101 tgccgaacgc catctccgag atggtgaggt agatccagga gcggacgtgc tcggtcaggt
     2161 tgacgtagtc ctgcacctcg gcgaagctcg ccgacttgat cagcgccagc gggttctcct
     2221 gcgcctcgat cgtgccgttc tgcagcgagg tgaagacctc ggagaacgcc atcggcgtcg
     2281 gctgggcgcc gagcgcgctc cagacgtcga cgaagagcgg cacgttcggc acgcgcatct
     2341 tcaggttgtt caggtcgtcg gggctcttga tcgggcggtt gctggtcagg ttgcgcgggc
     2401 cgcgggcgaa ataggcgatg gggcggatct gcgccttctc gacgatctgc gcctcgatct
     2461 ggtcgccgat ctcgccgccg gcgacgctgt ccatgtcctc gagcgagctg taggcgtaag
     2521 gcacggccag gagcgccgcc atcggcgccc agttctgcag gctctcgccg gtgatggtca
     2581 tgtcggcggt gccgagctgc atgccgttga tcaggtccat ttccttgccg agcgattcgt
     2641 tcgggaagac ctggacctcg atacggccgt cggtcagcgt ggcgacctcc tcgccgaact
     2701 tgagcgccgc cttgtgccag atgttgtcct cgttggcgag gtgaccgagc ttgagcgtca
     2761 cctcggcggc caggctggtg ttggccatga ggacgccgag catggcgccg agcagcgctg
     2821 cccggcgcgt tcctgcgagc tgcatgagaa ttcctccgcg tgtctgatgt tgctggtgcg
     2881 gggcgggtcg cggcatcgcg ggctgggcgt ccatttgtcg acctccctac cgcgacgacg
     2941 gctcgtcgcc tgggccgcgt gtagagcgaa cttccatact agtcaacagt aaaagaatct
     3001 gctacgccat ccctcgtgaa cgcgcggagg aggaagcgaa tgcgacagac atggcggtgg
     3061 ttcggcccca aggacctggt ctcgatcgac gacatgcttc aggcgggcgt cgagggcgtg
     3121 gtctcggccc tgcaccacgt cccgaccggg gccgtctgga gcccggccga gatcgagcag
     3181 cggcaggcgg agatcggccg ccggcgtgac ggcacgccct cgggcctcgc ctgggaggtg
     3241 gtcgagagcc tgcccgtctc cgaggacgtc aagaagcaga agggcgaatg gcgcgcccat
     3301 ctcgccaatt acggcgagag cctgagaaac ctggcggccg ccggcatcga ggtggtctgc
     3361 tacaacttca tgccggtgct cgactggacc cgcaccgacc tcacctacaa ggtcgcgcat
     3421 ggcggcacct gcatgcgttt cgacctcgtc gacttcgccg ccttcgatct cttcatcctg
     3481 gagcggcggg gtgcggcgga ggactatccc gagaccgtgc gggaggctgc ggccgagcgc
     3541 ttcgccggca tggacgacgg ccagaaggag cagctcaccg gcaacatcgc cttcggccta
     3601 cccggtgccg ccgagaccat gaccctcgac gacctgcgcc ggcacctcgc cgagtacggt
     3661 gcgatctcgg ccgagacgct gcgctcgcac ttcacggcct tcctcgaggc ggtcgtgccg
     3721 gtcgccgagg aggtcggcat caggctctgc tgccaccccg acgacccacc gtttccgctc
     3781 ctgggcctgc cccgggtcat gtcgaccgaa gccgactacg cggcgatcat gcaggcggtc
     3841 gacagccccg ccaacggcat cacgctctgc tcgggctcgc tcggcgcccg gccggacaac
     3901 gacctgcccg gcatgatgga gcgcctcggc gatcgcgtgc atttcctcca tctgcgcaac
     3961 gtctaccgcg agagcgacca ggtccagggc tccttctacg aggacgagca cctggccggc
     4021 gccaccgaca tggtggcgct gctcgccgcc gtgatcgccg aggagcgccg tcgcaaggcc
     4081 gccggccgcc gggatcacac gatccccatg cggcccgacc acggccagga catcctcgac
     4141 gatctcggcc gccgggcgca gcccggctac ccgtcgatcg gccgcctcaa gggcctggcc
     4201 gagctgcgcg gcgtcatgac ggcgctctcc cacccccggt ggggcatggc gtcttgacca
     4261 gccggctgag cctcgcgtcg ctgggcagca ttccggcgac ggtccgccgg ccgggcttcg
     4321 acccggccgg agcgggccac gggatcgtcc atctcggcgt cggcgccttc caccgcgccc
     4381 accaggccgt ctgcaccgac gacgccctgg ccgctgccgg cggcgactgg cggatccagg
     4441 gcgtcagccc gcgcagcacc gacatcgccg acgcgctgaa cccgcaggac gggctctaca
     4501 cgctcctgat ccgcggcccc gaaggcaccg aggcccggat cgtcggcagc ctcggccggg
     4561 tgatcgcggc agcccgcgag ccgggcgcgg cgctcgcggc catgagcgac ccggcgaccc
     4621 ggatcgtcag cctgacggtg accgagaagg cctacggcct cgatcgcgcc ggcggcggcg
     4681 tcctgcaggg ccaccccgcc atcgcccccg acctgcaatc gccccggcag ccgaagggtg
     4741 ccatcgggct cctggtcgag gcgctgcgcc ggcgtcgcca gcaggggctc gacccgttca
     4801 ccgtgctctg ctgcgacaac ctgccggaga acggcacgct gctgcgggcg ggcgtgctcg
     4861 acttcgccgg ccggaccgac ccggccctgg cccggtggat cgaggcccgg gtcgccttcc
     4921 cctcgaccat ggtggaccgc atcacgccgg ccgcgacacc ggcgacgctc gccgatgccg
     4981 cccggctcac cggctgcgag gaccaggccg ccatcgagac cgagcccttc agccagtggg
     5041 tcatcgagga ccggttcacg gcgggccgcc cggcctggga ggcgggcggg gcgatcttcg
     5101 tcgacgacgt cgcggcctac gagcggatga agctgcggat gctgaacggc acccactcga
     5161 tgctcgccta tgccggcttc ctcgccggtc accgttacgt gcgcgacgtc atgggcgacc
     5221 ctgtcctggc ccgcctcgtc cggcgccagc tcgccgccgc gaccggcacc ctgccgccgc
     5281 tcgccggcat cgatctcggc gactatgccg cggccctctg ccagcgcttc gccaacccgg
     5341 ccatcggcca cgagacccgg cagatcgcca cggacggcac cgagaagctg ccgcagcgac
     5401 tcctggcacc ggcgctcgag ctcagccggc ggggtggcga cctccgcccc ttcgccttcg
     5461 ccgtcgccgc ctggatgcgc tacgccctgg gccggaccga tgccggcgac gcctatgcgc
     5521 tccgcgatcc gcgcgaggcc gagatccgtg ctgccgtggc cggcggcggc acgccggcca
     5581 caatcgcccg ggccctgcac gggctgaaag gcttgttccc gcccgagctg gtcgccgatc
     5641 cggcatggac gagtgctgtc gaatcgattc tggctggcat gctcgggcgg ggcgtcgcca
     5701 ccgcccttgc cgaggaggcg gcccgcaccg cctgacgggc gtcgttcaat cgacgaacag
     5761 atccggatgc gcctcggcga tccgcggcag cgacttcagc aattcgccga gatgcgtccg
     5821 catcgcgcgc tccgctgcgt ccggatcgcc ggcctcgatg gcatcggtga tcgttcggtg
     5881 ctggtcgatc atgacatcga tcggcgtcgc tgccgggaag gagaggaagc gcacccgatc
     5941 catctgcgcc cgcagggtct cgagcagctt ccaggcgctc tcgcagccga tgctggccgc
     6001 gatcgcgcga tggagctcct cgtcctggcg caggaactcg accgtatcgt ccgcctgccg
     6061 ggcggcttcc tgccggtcga tgatctgccg caggagcgcg gtcgtgccgg gtgccgctgc
     6121 ctcggccgcc ttgcgggcga acgccgcctc gatggcctcg cggatgaacc gggcgttctc
     6181 gacctcgcgc cgcgagatca gctggacgaa cgtgccgcgc tgcggccgga tctcgacgag
     6241 ccccgcctcc gcgagcttga tgaaggcctc ccgcaccggc tgccgcgaca cgccgaactg
     6301 ccgtgccagc tcggtctccg acagcaggtt gccgggccgc agcctgagct ggatgatggc
     6361 ctcgcgcagc aggtcgtgga cccggctgct catgctgccc tggcggaagt tcgccaccgc
     6421 ggcgacagct tcgtcgggga aggccatggt catgcgaaag tccctgcagg tcgaagggcc
     6481 gggatggcca ggcctgcata ctaccattca acagcttggg caattcgcac gaagccaggg
     6541 ccggtgctca gcgcagatgc gtgtcgaaga acgccagcgt ccgctcccag gcgagggtcg
     6601 cggcggcctc gtcgtagcgc ggcgtcgtgt cgttgtggaa gccgtggttc acgtcgggat
     6661 agaaatggac cgtgaattcc ttgccgttcg cctcgagcgc tgcgccatag gcttcccagc
     6721 ccgcgttgat gcgctcgtcc agcccggcat agtgcaggag caggggggcc tcgatggcag
     6781 gcacgtcctc gaccgccggc tgccggccat agaacggcac cgacgcggcg aggtcgggat
     6841 aggcgaccgc gagggcattg cagaccccgc cgccatagca gaacccgacg gcaccgacct
     6901 tgccggtcga cgcctcgtga tcccgaaggt gctcgaacgc ggcgaagaag tccgccatca
     6961 gcttggcccc gtcgagccgc tgctgcatct cgcgaccctc ttcgtcattg ccgggatagc
     7021 cacccagggg cgtcagcccg tccggcgcga aggcgagata gccctccttg gccagtcgcc
     7081 gcgcgacatc ctcgatatag gggttgaggc cgcgattctc gtggacgacc agcacggcgg
     7141 gcagggggcc cgaggcatcc gccgggcggg ccatgagccc ggccatcgtg ccgtgcccct
     7201 ccgggctctc gtactcgacc ctttccgtca cgatggccgg atcgtcgggt gcgacctgct
     7261 gggccagcgc atagttcggc tgaagctcgt ccaggaccat ggcccccgtc acccccacgg
     7321 cggtgtactt gccggcccgg gcgatgaact cgcgtttcga catcctgccg tgcacatagc
     7381 cgtcgtagaa ctcgagaatg cgctggtcga agtcccgggc gctctttctt tgggggctgg
     7441 tcaatctgtc ctcctggggt tcgcgtgttg ccatcgcgag tttttcctct gctacgacca
     7501 tcgggcgagg tcggcagacg tccgtggcga tggacatgcc aggaccaaac taggtgcggg
     7561 accgcctccg gcaattgccg gagaggcaat tcggcccggg cacacaacaa gcaatgtcgg
     7621 cgggaaacgt cgccagagtt tgtcggtggg cgaacggttt gtatcgaaat gccggctgca
     7681 gcgagcgccg ccgggccgtg ccccccttgg atggcgcgcc ctgcaggact cgaacctgcg
     7741 acctgctgct tagaaggcag ctgctctatc cagctgagct aagggcgccc ggcggcgctt
     7801 atagcagggc cgtggtctcg gggaaacggc tggccgggct caggtgcggc ggatgaagtt
     7861 ctcggcgtag gacttgggct ggacgacgcg cacatgcggc tcgaagagct ggaaggtcag
     7921 gttctcgcgc cggcaatagg cgatggcggc atccttggtc ggaaagcgca gccgcacctg
     7981 ctgggccgtg tcgtccgagc cgttccagcc gatcagccgg tcggcctcga cccgcgagcg
     8041 cggctcgaac tcgacgagcc actcgtgggt cttgcgccgg cccgactgga ccgcggtctt
     8101 cgccggtcga tagatccgtg cctgcatcgc catggttcgt gcaatcgctt ggggaatggc
     8161 caggagctag gcaatttttt cggattccgc aagccagcgg cggcgcagct ccgtgtggtg
     8221 ggcctgcagc cagcgaagcg ggatgatggt gttggcggcg aggatccggc cgttctcgag
     8281 ccagtcgaac gcggtctgga gcgggaccac gaagctcctg atgtcctccg cctcgtgctc
     8341 caggccgtgc aggccgccgg cgccgctggc gtcgacctcg gcgatgaagc aggtgctgcg
     8401 ctcggtcacc gccccgggcg aggcgacgta ggtcgcgatc ttctcgatcc gtcgcgcctc
     8461 gagcccggcc tcctcgacca gctcgcggcg cgcggtctcg acggcgcatt cgccgggccc
     8521 gacgagcccg gccaccacct cgatgagcca ggcgccggtc ggatggcgca tgcagccggc
     8581 ccggaactgc tcgatcagca cgatgctgtc gatgaccggg tcgtagggca ggaccgcggc
     8641 cgcctccggt atgtggagga tctcgcgggt gatggtcggg ctcatctgcc cgtcgaagcg
     8701 gcgatgcttc agatccacga cctcgaagcg gaagaagccc cgccaggcgt cgcggctcgc
     8761 gacgatccgc acctcgcggc cctcgtcggc caggggcagg tcgtcggcca gcgccgcggc
     8821 cgcgctctcc gcccggtcgg tcaagcgaag gccttgaagg tgatgagggt gaaggtgtcc
     8881 ttgacgccgg gcagggtctg caccgccttg ttgacgaagc ggccgatatc gacgtcggcg
     8941 gcgagatgga acttcatgat caggtcgtac tggccggaga ccgagtagac ctcggagacc
     9001 tcctcgatgg tctcgaccgc cttgccggcc acctcgtagg agcagccgag ctcgcacttg
     9061 accatgacga agatcgtttg catgggcggc tcccggtaaa gcgggcgcgg tttcgggtca
     9121 ggccacggcc ttgatcggca ccggccgcag gaggaaggcg gggacgtgat cgccgagccc
     9181 gaccacgggc tcgtcgcgcg gctcgccgcg gccccgctcc ggcttcgacg ccggtgctgc
     9241 ggccttggcc acgcccgtcg gcggtgcatc ggcggcggcc ttgcgccggc gcggccggct
     9301 cttcgccggc tccgcctcgg cttccggcgt gtcgccggcg gccggggtct cggccgttgc
     9361 ttccacggcc accggctcgg ccgcgacctc cgccgtctcc gggcgacgcc gccgccggcg
     9421 ggtgccggcc ggctcggccg gggcagcttc ggtcagctca gcttcgatcg ggccagcttc
     9481 agttgcggca gcttcggtcg ggctgggcac cgccgctgcc gaggcttcgg gtgccgcctc
     9541 cggcgcgctg cgccgcttgc gtcggcgcgg ggccggtgcc gcgtcgctct ccgctgcggc
     9601 tgccggtgcc ggtgccgctt ccgccgacac tgcctcggcc tcgcgctcgg gggcacgccg
     9661 cgccggcctt gcccggctcg gccgccgtgg cgcctcggcc gcgtcgctca ggctctcgat
     9721 cagcttgcgc tcgagcttgc tgccgagcag ccgctcgatc gcggccagat acttggcgtc
     9781 gtcggggtcg gccagggtgt aggcgtggcc ctccttgccg gcgcgcccgg tgcggccgat
     9841 gcggtggaca tagtcgtcgg gatggatcgg cacgtcgaga ttgatgacga cgggcagatc
     9901 gacgatgtcg aggccgcgcg ccgccacgtc ggtggcgacg aggaagtcga ccgtgccggc
     9961 cttgaaggcg tcgagcgtcg cctggcgctg gctctggtcg agatcgccgt ggatgtcctg
    10021 ggcgttgcgg ccctggcgct gcaggaactt gttgacggac gagacgtcgc gcttgcggtt
    10081 gcagaagatg atggcgttct cgatcttctc gacctcgagc agccggtcga gggcgacgcg
    10141 gcgctcgatg ccgcggatgg gcaccacgac gtcgatcacc ttctcggccc gggtggaggg
    10201 gcgggcgacc tcgatctcgg ccgggtcgcg caggaagcgc tcggcgaggc ggcgcacctc
    10261 cttcggcatg gtggccgaga agagcagggt ctggccgcgc ttggtcaaaa gctggaagat
    10321 ccgctcgaca tcggggatga agcccatgtc gagcatccgg tcggcctcgt cgatgacgaa
    10381 gacctcgacc ccgttgagca ggatgcggcc gcgatcgaac cagtcgagca gccggccggg
    10441 ggtggcgatg aggacgtcga cgcccttctc gagcagcttc tcctgctcgc ccatgcccac
    10501 accgccgatc aggagcgcca tggtcaggtt gagatgcttg ccgtaggtct cgaagctctg
    10561 cgagatctgg gtcgccaatt cgcgcgtcgg gctgaggatc agcgagcgcg gcatccgggc
    10621 cctggcgcgc ccggcggcga gccggtgcag catcggcagg gtgaagcccg ccgtcttgcc
    10681 ggtgccggtc tgggcgatgc cgacgacgtc gcggccgtcg agcacggccg gaatcgcggc
    10741 ggcctgaatc ggggtcggat gcacatagcc cgcgtcggcg atggcacgct gaatttcctc
    10801 gcgcagcccg agctccgcga aggtgatggt cgcttgttcg gtggtcaaga acagtccttg
    10861 gtgggtgaca tcggatgccg gccttgcatg tatagacgat cgttgtggag tcaatgacaa
    10921 gcggtcgcgc aacatcccgt cttgtgcaaa aaagcggcag gatcggcgaa gaagtcgcgg
    10981 gagcgagggc atgatgcgtg gaaaagcggg atgatgttga gccggagcga atcgctgctc
    11041 ttgattgtcg acgtgcaggc ggggctggcc cgggcggtcc atgatgcggg ctcgcgcatc
    11101 acgaggctcg ccctgctgct gcgggcggcg gcggtgctgg acgtgccggt catcgtctcg
    11161 gagcagtatc cgaagggcct cggggccacc gacccgcgcc tcctgccgct gccggacggc
    11221 gccaccgtcc tgcccaagct cgccttctcc tgctggcgcg agcccgtgct gcgggcagcg
    11281 ctcgagcggg ccggccggcg gcaggtcgtg gtcgccggca tggagaccca tgtctgcgtg
    11341 ctgcagacgg cgctcgatct ccaggaagcg ggcttcgccg tgcatctcgc ggccgacgcc
    11401 tcgggctcgc ggcgggtgga ggaccgtgcc ctgggccagg cgcgcctgcg cgatcggggg
    11461 gtggcggtcg tgaccgccga gatggtcgtc ttcgaatggc tggagcgggc cgaggggccg
    11521 gccttcaaga cgctgatccc cgcgatcaag gccctgggca cgaccgatcc gggcgcagcg
    11581 cgcggccgcg actgacggaa ttcaggcgct gccggccctg gcctcggcga tccggtggtc
    11641 gaggcggtcg tcctcgagcc cggccggctc ctggtggatg atgatctcgg tctcgccgaa
    11701 agcctccgcc agccgtgcct cgagggcatc cgtgacgcgg tgcgcccgac gcacgctcat
    11761 cgccccgtcg atctcgacat gcagctcgat gaaggtggtg ttgccggccc gcctcgtgcg
    11821 caggtcgtgc aggtcgacga catcggggtg gtcgcgcacc agcgcctcga tgcgccgccg
    11881 ctcggcgccg ctgagctcgc ggtccatgag ggtgtcgatc gccgagcggc cgaggcgggc
    11941 ggcctgccag gcgagataga gggcgaccag cacggcggcc accgggtcga tccaggcgac
    12001 gtcgagcccg gccgtcaggg ccagggcggc gagcacgagg aggttggtcg cgaggtcggc
    12061 cgtgtagtgc agccggtcgg cggcgatggc ggtcgagccc gtgcggcgga tgacgacgcg
    12121 ctgcagggtg accagcgcca gggtgatcag gatggcgccc acgaggacga tgaaggcgaa
    12181 aagcccgggc tcgatgaccc tggggctcgc gagccgctcg gcggcgccga tcagcacgaa
    12241 gagggccgag cccatgacca gcgaggcctg ggccagggcg gagaggctct ccgccttgcc
    12301 gtgcccgaag cggtggctgc ggtcgggcgg ctgctggctg acccggacgg cgaggaaggt
    12361 gatcagcgag ccgccgatgt cggcgaggct gtcgatcatc gaggcgagca ctgccagcga
    12421 atcggaggcg agggcggcgg cgagcttgcc cagcgtcaag agcaccgcga ccgtcaggct
    12481 ggccccggcg gcgagcctga cgagcgctgc cgggtcgagc cgctcgagca tggccaaggt
    12541 ttagtagagc tggcgctcgt aggcgtcctc gatcagcgcg tcggtggcct tctcctcgat
    12601 cccctcgatc tggtcggcga ggatccgcgc caggctcggg aactggccct tcgcgatgtg
    12661 ctgggtcggg tcccagagct tcgagcggat cagcgccttg ccgcaatgca tgaaggcctc
    12721 ctcgacgctg accacgatca ccgaccgcgg cgccttgccg gcatgcgccg tcgcctcgag
    12781 cagctcgggc tcggtgctga tcctggccgt gccgttggcg cggaaggtct ccttgtagcc
    12841 gggcaggaag aagatcagcc cgaccctggg gttcgccacg atgttctgca ggctgtcgac
    12901 ccggttgttg cccggccggt cggggatcag gagcgtgcgc tcgtccagca ccttgacgaa
    12961 gcccggcgca tcgcccttgg gcgaggcgtc gcagcggccc tcggcatcgg cggtcgccag
    13021 gacgacgagg ggcgagagct cgatgaaccg gcggccgtgc cggtcgatcc ggtccatctc
    13081 cttgcccgag gcggtccggc tgacgctgcc gtaatgggcg cggagcgcgt ctctgtcggt
    13141 gagattcatg gtcatggacg ggctcccgat ccggtgtggc gtgcggccct gcgcgtcgca
    13201 cgtccgccgg gaaatggcaa ggtcgagcag ggccgccgcc gcggtcctca gagcgtttcc
    13261 agcagcttcc tgctctcgac gaggtcgggc agggcgaagc cctcggtgaa ccagccgagg
    13321 accggcgcca ggagatccag cgcctcctgc cgcctgcctt gcccggcgcg cagccgcgcc
    13381 aggtcgcggg cggcgcgcag ctcccagagc ttcgcctcct gcccgcggct gatctcgagg
    13441 gccgcgtcga gatggcgccc ggccgtgccc gcgtcgtcgg cgcagccggc gagcaggcgg
    13501 tgcagctcgg catcgaacca gccttcgccg gtcgccgcgt tccgcgccag gccctgctcg
    13561 agcagcgccc gcgcctcggc gatccggccg gcctgttgca gcacatcggc gcgcaggctc
    13621 gagagatagg cgagctcgat gtggtgcgcg gctgagtcga gcgccgcgag cgctgccgcc
    13681 atcatggcgt cgccgcgccc ggcatcgccg cccttgccgg cgagccagcc gacgaagacc
    13741 gaggcgatgt tctcgtaaat ggtcaggtgc gcgtttcgac agagctccag gcaccgactc
    13801 gcgcgctccg agagaagccc catcctgccg gtgaggcacg gaatgtagca gccgttgccg
    13861 agcgctgcgg cgagcacgaa cttgcgcccc atgccggcgg cgacctgcag atgggccgct
    13921 tcctgccggg ccgcctcatc ggggtagccg aggacgagca gagaccacgg gagatagatg
    13981 cgtcccacgg tcgggcgatc ggggtagggg ccctcgcctt ccggcagggc ttcgagatgc
    14041 cgtcgcgcgg ccgcgaatct gccgcgcacg aaatccagga ttcccgcggc ctcgtgacgg
    14101 tgccagagac cggccggtcc agcaccttgc gagggcgcgg cccgcaagag gccggcgacc
    14161 tcgtcggccc ggtcgaggcg ggcggcattg aagtgggaca ggaactcacc gaagagcgcc
    14221 atctcctgcc cgtcctggtc gccgatctcg gcataggcga gacgtgagcc ccggtaggcc
    14281 gcggcggtgg ccggtgccgc cgtcccgagc gtggtgcgca ccgaatcgcc catcagcaga
    14341 tggagcttcg cccgccagcc cgtagcggtc tcgtcggccg gggtgcgccc gaccgccgcg
    14401 aggccggtct cgatcagctc ccgcgcctcc ggtgccgcgg cgcgggcgat ggcggcgtcg
    14461 atggccggca gcagatgcgg catggcggcg gccggctcgt cggccgcgag gaaatgccgg
    14521 gcgacgagct gcggctcgac gccgccgtgc ccggccgcct tgtcggccag caccctggcg
    14581 atgcggccat gcacctcgcg ccgccggctg cgtagcaggg attcgtaggc cgcgtcccgc
    14641 accagcgcat gcttgaagac gagccggcct tccgccggcg gcccctggcg gaagacgagc
    14701 tccgcccgga ccagccggtc gatcgcccgc tcgacctcgg ccgcgggccg cccggcgacg
    14761 gcggcgagca ggccctggtc gagctcgcgg ccgatgcagg cggcgacctg cgccagctcc
    14821 ttggcttccg gcagccggtc gaggcgggcc atgagcgaat cgtggagcga ggccgggacg
    14881 gcggcatcgt ccgattcgag gaccgccttg gtcagttcct cgacgaagag cggcaccccg
    14941 tcggtgcgcc cgacgatggt ctcgatcgtg ccggccggca ggccatcgcc gccgagccgg
    15001 cggatgatcg cctcgacacc ggcgcggccg agccggtcga gggccagccg ctcgagcggc
    15061 gccgccgtgc cccattccgg caggccctcc ggccggctgg tgatcagcac gagcagccgc
    15121 agccgcccga gcccggcgag gaccgttgcg atcagctccc gcgtcgtcgg gtcgagccag
    15181 tgcgcgtcct cgaccaccac caggagcggc ccgctgcggg cgaggccgag cagccggtcg
    15241 gccagcgcct cgcgggtccg cgcccgccgc gcctgcggcg tcagctcgag ccgcccgtag
    15301 cgggcagcac cgtcgagccc gaggagctcg gcgatcagcg gcagggcgtc gcgctgctcc
    15361 gggaacccca ccggcagcgc ctcgagccgg tcgagcagca cgtcgttcgg ctggccatcc
    15421 gcgaagccgg cctcgtcccg caattgctgg atcacgggcc agagggcact gcccgcatgg
    15481 tagggcgagc cctgcaggcg aacgagtgtg tacgattcgc ccgccagcgc atcgccgagg
    15541 gcgtggacga tccgggactt gccgtgcccc gcctcgccga ccagcagcac ggcctgtccg
    15601 gcaccgtccc tggcccgccg ccagcgttcg agcagcaggg cgaactcccg ctcgcggccg
    15661 accatgggct ggagatggcc ggcgcgcgcc tcgaagcggc tggccaccac ccgctcgcca
    15721 ttgaccgcga agaccggcac tggcccgtcg atgcccttga gcgcccgatc gccgagctcc
    15781 ctcacatcga agcctgcccc gagcagccgg cgggtcgagc gggcgaccag cacctggccc
    15841 ggcccggcca gggcctgcag ccgggccgcc aggttcggcg tgtcgccgac caccgtctgc
    15901 tcgctggcgc taccgtcgcc gatcggctcg ccgaccacga cgagaccggt ggcgatgccg
    15961 acccggcagg cgaggggggg atcgtgcccg gcgagccccg ccaccgcctc gatcaccgcg
    16021 agccccgccc tcacggcccg ctcggcctcg tcctcgtgcg cccggggcca gccgaaatag
    16081 gccagcaccc cgtcgcccat gaacttcgcc acatgtccct cgaacgccgc gacctcgccc
    16141 gccacggcac tctgataggc gcgcaggatc ccacccatct cctccgggtc gagccgcgcc
    16201 gagagcgccg tcgagccgac caggtcgacg aacatcaccg tcaactggcg acgctcggcg
    16261 gccggccccg gggccggtgc ctcggccgcc ggcgagcgat ccgtgcgcct gccgaggtgg
    16321 gcaatggccg cgagcagccg gcggcgatgg ccgaccgagg tcacgccgat ctcccgcagg
    16381 tcctcggccg tcaggctggc gaggaggtcg gcgtcgacgt cgttcgcgcg aaaggcttcc
    16441 acgtactggc cgaggccgag ctcgcgaagc caggacgcca tgtccaatgc cgtctccatg
    16501 gtcgacggtt catggcaacc ctaccctgcc cggggcggcc cggccacagt gctgccggtc
    16561 ggcctgccgt ctcagctgcc gcggggcggc accgcgacgg gtgtcggccg ccatgctgcc
    16621 gccagccgct ccgcctcggc gatctgctcc gggcgcatcc tttccatcag ctcggcccga
    16681 aacttgctgg cacgggcggc gatcgtcaga tcggcgggcg gggtcgcggc gagcgtcacc
    16741 catttgagcg cctccacggg atcgggctcg acaccctggc cctcggcgta gagcgtgccg
    16801 agatagaact gcgccggccg gtggccctgg gccgccgcca tgcggaacca gtgcgcggcc
    16861 tggcggtcat cctgcggcac gccgagcccg gtatagtaca tgccacccag attgtgctgc
    16921 gccggcgggt cgccctgcat ggcggccagc ctgaaccagc gcgccgcctc ctcgtcgttg
    16981 cgcgccacgg ccacgccctg ggcatagaac ccgcccagcc ggttctgcgc ctccggcagg
    17041 ccctggacgg ccgccgccct gagccagcgc agcgccgccg cctcgtccgg ctggacgccg
    17101 cgccccatca gatagagcag gctcatgttg tactgcgccg gcgcatgccc ggcctcggcg
    17161 gcggtgagga accagcgcgc cgcggcctcg tcatcggcca ccacgccacg gccgtcgaga
    17221 tagcggtcgg cgagatcgaa ctgggccttt ggatcgccgg cctcggccgc ccgcagcagg
    17281 gccggcacgt tggtcacgcg cggggccgat gccccggggc tggcggccgg ggggacagcg
    17341 gccggcgggg ccgcgggcgc tggcgctggc gctgcgacct cgggtggcgc cgcctcgccg
    17401 gccgccgggg cttcgaagcg ctcccgcaag ccggcgcagg ccgccagcag caggccggcg
    17461 caggcaacca gcagcaggcc ggccgtgagg cggccctgaa ccatcagctc gtctcctccc
    17521 ccatcgcccg accgttcggg cgatcatcgc cccggctaac ccaagggcgg tgcagcggca
    17581 agcccctccg tcgcagcgcg ccgcgaagtc gcgctgcgcc tagacccagg cacgcagcag
    17641 cgccgcgagg ccgatgccca ccgccatcgc cagcagggcg ttccgggtcc ggcgcatgac
    17701 cagcaccgcg gccaggatcg cgaggatggc ggccgcgccg ctcgtcaccg cggccgggac
    17761 gatgatggcg atcagcaccg agcccgagag atggcgcagg aacgcctcga gccggggcga
    17821 cagcggcacc atggacatca ggagaaagcc gccggcccgg gtggcgtagg tcaggaccgc
    17881 catggcgctg atggcgagga acgcgtcagc gtcgtgcatc gaccaccacc ccgaccaggc
    17941 cgccggccac ggccccggcc aggatgtgcc agccggccgg caggatcgcc atggcagcca
    18001 gcgccgtgat tccggccgcg agccacgcca ggtcgtccga ccggccccgc cgcagcccgg
    18061 tgagcatgca ggaaagaaag gcgaggaaga cgagatcgag cccgaagcgg cgaaaatgct
    18121 cgggcgagag atcgatgaag ccggagccgg cgatggtgcc ggcgacccag gcgacccaga
    18181 gcaggacgcc gccgccgatc aagacgccga catcgcgctc gccgcgcgcc tcggcctcga
    18241 tcgccttggc ccagttcggc tcgctcagca gcaccgccgc cagatagacc tgccaggcgg
    18301 gcaggctgcg cagccaggga tagagcgagg cgccgagcag gaaatggcgc ccgttgacgg
    18361 cgagcgtggc gatcaggagc gggccccacg ccaccggcga ctgccagagc tcgagcacgg
    18421 cgaactgcga gccgccggcg aagacggcag cgctcatcag gttggcggcc cagccctcca
    18481 tcccgacatt ccgggccgct atcccgaaag cgacgccgaa gacgaaggca taggcgccga
    18541 cggcgacgag ctcgcgggcg ccacggccca tgccgcgcca ggtgatcacc gcccgctcca
    18601 tccaccgccc ccgtcctcga agatgcggcg ttcagagcac gggccgcggg ccggggcaac
    18661 caccgctctt tatccccgcg tgcagttgcg ccacgatgcg ccgcaacgca ggcggagcag
    18721 caacaaggag caagatcccg atgacccaga acctgaagcg ccgcaccatg ctcgccggga
    18781 cagccgcgat cggcgcctcg gcgaccaccg cggcgagctt ccccaggccc gccatcagcc
    18841 agggggtcac gcgctggaag atggtcaccg cctggccgaa gaacctgccg ggcccgggcg
    18901 tcgccgccca gatgctggcc gaccgcatct ccgccctctc cggcggccgg ctcgaggtcg
    18961 agctcttcgc cacgggcgag atcgtccccg gcaaccaggt cttcgacgcc gtggccgagg
    19021 gcacggccga gctctatcac gccgtcccct cctactgggg ctccaagtcc aggggtattc
    19081 tgctcttcgg ctcccagccc ttcggcctgc tggccaccga gcaggtcggc tggctgaccc
    19141 atggcggcgg ccaggcgctc tacgacgaga tctacggccg cttcggcctc aagcccttcc
    19201 tctgcggcaa tagcgggccg caatgggccg gctggttcag gaacgagatc acctccgtcg
    19261 acgacctcca gggcctgcgc ttccgcacca ccggcctagc gtccgagatg tgctcgaagc
    19321 tcggcatggc cgtacaggcc atgggcggcg gcgacatgtt ccaggcgctg cagtccggca
    19381 ccatcgacgc cggcgagttc atcggcccct ggaccgattc ggcgctcggc ttctaccagg
    19441 tcgccaagaa ctactactgg ccgggcgtcg gcgagccctc ctcggccgag gaatgcgtcg
    19501 tcaacatggc cgcctgggaa gccctgccgg acgacctcaa gctcgccgtc gagaccgcct
    19561 gcaagtcgct ctacaacgac gtgctcaccg aatactcgac caagagcgcg cagagcctgc
    19621 cgcagctcgt cgccgagcac ggcgtcatgg tccgccagct ccccgaggag gtgatgatcg
    19681 ccatgggcaa tgccgcgggc gaggtgatgg ccgagctcgg cgacgaccag gacgagctgg
    19741 tccgccgcat cgtcgagagc ttcctcgcct accgcgcctc gatcgcgtcc tacatggtct
    19801 atgccgacaa cggccagatg aacgcccggg cgctgccgtt cacctacggc tgaccccggg
    19861 ccggctcggg agcccgggtg ctcgccaccg ccgccgacgt gctcgaccgg gtgaaccgcg
    19921 cggtcgggca cgcggcgcgc tggctcgccc tcctcatgct gctcgcccag ttcctggtgg
    19981 tggtgctgcg ctacctcttc ggcaccagct acatctgggg caccgagacc gtcctctatc
    20041 tgcacgccgc cctcttcatg ctgggcgccg gctacacgct gctcgtcgac ggccatgtcc
    20101 gggtcgacat cctctatgcc gggctcggcg agcggggcaa agcgctggtc gacctgctcg
    20161 gcacgctcct cttcctcctg cctacctgcc tcgtcatcct ctggttcacc tggcgcttca
    20221 ccgccaacag ctgggccatc cgcgaaggcc cgctctcggt cggcggcatc ccggcctcct
    20281 tcctgctgaa gaccctgatc ccggccttcg ccctcctcct cctgatccag ggcatcagcc
    20341 tggcgctgcg ctccctcgtt cgcctgcagg gcggcgacag ccggtgatcg ccgcgaacct
    20401 cgacctcgtc atgctggcgg ccctctgcct cgtcatcctg acaggctacc cggtcacctt
    20461 cctcatcgcc gggctcggcg tgctcttcgc cttcctcggc tgggccatgg gcgtcttcga
    20521 cctgggtctt ctgggcgcgc tcgcccagcg catcttcggc gtcatgacca accaggtgct
    20581 gatcgccatc ccgctcttcg tgctgatggg caccatcctc gagcgctcgc gcatcgccga
    20641 ggagctgctc gagcagatgg gccggctctt cggcaccatc aggggcgggc tcgccgtctc
    20701 cgtcaccctg gtcggcgccc tcctcgccgc ctccaccggc atcgtcggcg ccaccgtcgt
    20761 caccatgggg ctcatagcac tgccggccat gctgaggaac ggctacggcc ggggcttcgc
    20821 ggcaggttcg gtggtcaccg ccggcacgct cggccagatc atcccgccgt ccaccatgct
    20881 catcatcctc gccgacgtca tgtccaccgc cttccagcag gcgcagtaca cccagggcaa
    20941 gttctcggtc gagacgatca gcgtcggcca ggtcttcgcc gccgccatcc tgccgggcct
    21001 gctcctggtc ggcctctaca tcgcctacat cctctggacc gcgaagcgcc gcccggacct
    21061 cgccccggcc atgccggcga ccgacgagaa gccgagcccc gccgccgtcc tcaaggccct
    21121 cgtcccgccc atcgtgctga tcgtcgccgt gctcggctcc atcctcggcg gcatcgccac
    21181 ccccaccgag gccgcctcgg tcggcgccat cggtgcgctc ctgctcgccg gcctccgcct
    21241 cgcccccgat cgctccggct ggcccgtctg gctcgccatc gccgccatcg tcgccctcct
    21301 ggtgctgaca tccttcgtcg acctccgcct cggccgcagc ctgtccacca ccacggagag
    21361 cctcgccatc tggctcgcct tcggcctcgt ggcgctgctc gccatcggcc tcgtccaatg
    21421 cctcgtgcgc gcccagcgca ccggcatcct ggtgccggtc ctgacccaga ccatgaccat
    21481 cacctcgatg atcttcggca ccatcatcgg cgcctcgatc ttctccctcg tgctgcgcgg
    21541 cctcggcggc gacgcccgca tcgagggcct gatcgaggcg ctgccgggcg ggcccggcct
    21601 cgccctcctc ttcgtcatgg ccgccgtctt cctgctcggc ttcttcctcg acttcgtcga
    21661 gatcagcatc atcatcctgc cgctgaccgt gccgatcctg ctgctcatgg gcttcgaccc
    21721 gatctggctc gccgtgctga tcgccatcaa cctgcagacc agcttcctca ccccgccctt
    21781 cggcttctcg ctcttctacc tcagaggtgc ggcgccgccc gagatcacca cgggcgacat
    21841 ctacaagggt gtgatcccct tcatcctcct gcagatcctg gccatcctgg tcgtctacct
    21901 cgccccgccc atcgccacgg tcctgcccgg cctgctcttc tgaccggctc tcgcaatttc
    21961 cgaatttccg attatctgac attcaagcaa gccaccgcca gccgaggccc gccatgaccc
    22021 tcgtcaagct ccgcaagagt gcccagctca ccttgccgac cgaaattcgc gaagccctca
    22081 agctcgccga gggcgactac ctcgaagccg agatcgtccc gggcggcgtc ctcctccgcc
    22141 ccgtcgacgt cgtccgccgc gaggcggcgt gggacgacct cgtcgagctc gtcgagacgc
    22201 cgaagtggcg aacgccgccg accgagtcgc ccgaggagga agaggccctg atcttcaaaa
    22261 tggtcgagga ggctcgccat tcgcgcgact agggtggttc tcgacagctc cgtcctggtc
    22321 agcgccttcc tcaccccacg cggcacatcc gcgacggtcc tggtcgcgac gcggcaagcc
    22381 cggttcaccc tctgcctctc gcgcgagatc gtcaccgaaa ccaccacgag gttgatccgc
    22441 aagctgccgc ggcttgcacc aaaatacgcg tacaccgaag cggacgttgc ccgcttcgcc
    22501 cgtgatctcg ccgagaccgc cgcgttcgtg accgacctgc ccgagctgga cgccgtgccc
    22561 ctggatcctg acgacgacat catcatcgcc accgccgtcg ccgctcgcgc cgacctcctc
    22621 gtcaccggcg accgccacct cctcgacctc ggaacccacg acgccatccg catcgtcgac
    22681 ccgcgccgct tcctggacga gctcgcaaag gcccgccccg gtgccggctg aggctgcata
    22741 acggcgccat ccctgctaac ctgcgcccgc ccagcaaccc ggcctcgcgg agcccgccca
    22801 tgaccctcac cctctacggc gtcgcccgct cgcgcgcctc gcgcaacatc tggctctgcc
    22861 tcgaggccga cctgccgttc gagcaggtcc ccgtcatcca ggcccgtcgc gtgaaggacc
    22921 cgctcgcgcc cgacgccccg ctcaacacgc gctcgcccgc cttcctcgcc atcaacccca
    22981 acggcctcgt ccccgccatg cgggacggcg agctcgtcct cttcgaatcc ctggccatca
    23041 acctctacct cgcgaggaag accggcgccc cgctcggccc gaaggacctc gccgaggaag
    23101 gccggatcct catgtggagc ctctggtccg ccaccgaggc ggagccctgg gcgatccaga
    23161 tcctcatgca ccgcgcctac ctgcccgagc ccgagcgcga ccccgccgcc gccacgcgcg
    23221 cggctcaggc cctcgtcccg aaattcgccg tcctcgatca gcacctgaag ggccgcgact
    23281 ggctcgtcgc cgaccgcttc accgtcgccg acatcaacac cgccgaggtg ctgcgctacg
    23341 ccgccctcgc caccgagctc ttcgaccagg cccccaacgt caagcgctgg ttcaaggcct
    23401 gccaggcccg ccccgccttc aaggaaatga tgcgccgccg ggaagcggag ccggcctgac
    23461 caagcggcgg gggaaccgag ttctcccgcg ccccgcgcag cgacggccgg gacgcgggtg
    23521 gtcgagagcc aggccaggcc tccgcgcttg cttccgagag gcgcccacac atgtcccatg
    23581 gagcaataca aggaaagttg tccatggctc gatcgaagac cgtcaaccgt caatgggtgc
    23641 tggcggaacg cccgaagggc gaacccgatg accggacgtt gcggctggtc acgggcgacg
    23701 tcccgacccc cggcaagggc cagatgctgc tgcgcaacga gtacctgtcg ctcgacccct
    23761 acatgcgcgg ccggatgagc gaccggccat cctatgcgcc acccgtcgaa ttgggtgccg
    23821 tcatggtggg cggcaccgtg gccgaggtcc tcgggtccga cctcgacgcc ttcgccccgg
    23881 gcgaccgggt cgtggcttcc ggcggctggc aggattacgc cctctccgac ggcaccggcg
    23941 tgatcaacct cggcccgaac ccgaagaacc cctcctgggc gctcggcgtg ctcggcatgc
    24001 ccggcctcac ggcctgggcg gggctgacgg agatcggccg gcccaaggcg ggcgagaccc
    24061 tcgtcgtcgc cgccgccagc ggcccggtcg gcgccacggt cggccaggtc ggcaagatcc
    24121 tcggctgtcg cgtggtcggc atcgccggcg gcgccaaaaa atgcgcccac gtggtcgatg
    24181 ccttgggctt cgatgcctgc atcgaccatc gggccgacgg cttcgccgat gccctgaagg
    24241 cggcggtgcc ggacggcatc gacatctatt tcgagaatgt cggcggggcc gtgctcgacg
    24301 ccgtgctgcc gctcctcaac cccaaggccc gcatccccgt ctgcggcctg gtctcgatct
    24361 acaacgccac ctcgctgccc gacggccccg accggctggg ccagctgatg atgatgatcc
    24421 tgcgcaagcg catgaccctg cagggattca tcgtcttcag cgatttcggc cacctctacc
    24481 cgaaattcgc gagccagatg ggcgcctggg tcgaggcggg cagggtccgc tacctcgagg
    24541 aaatcatcga aggcctggag caggcacccg ccgccttgat cggcctgctc aagggcgagt
    24601 cgttcggcaa gcgcgtgatc cgcctggcat cctgaccggc ctgctcgccg tcacctggcc
    24661 ggaaccgcca gccgggtcac cgcggaggtc ctgcggacga caggctgagg ccggggcgag
    24721 ccctgctgct ccgaacgaga atggcggggg aaccgagttc ccccgcaccc cttccgacct
    24781 tcagcgggac gtcgtcgacc taagcggcct tcctcgcctt cgcccggtcg ttcgccgaag
    24841 cgtcggcgag cttggtcaga tcgctgtccg tcctcgactc ctcctgcagc gtggcatcca
    24901 agagcttggc ggcgtcgtcc atgccgagct ccctggccca gcgcttcagg gtgccatagc
    24961 gggcgatctc gtagtgctcg acggcctggg cggcggagat cagcccggcg tccagcgccg
    25021 cggtgtcctt gaactcctcg atgatctcct cgccttcggc gatgatgccc tcgatcgcgt
    25081 cgcaggtctt gccctgcgcc cgcttgccga tgatgtcgaa cacctgctgc aggcgctcga
    25141 catgaccttc ggtctcctgc ttgtgcttgt cgaaggcggc cttgaggtca tcggactggg
    25201 ccgcccgctt catcttgggc agcgccttca ggatcttgcg ttcggcgtag tagatgtccc
    25261 tcagcgtatc gtggaagaga tcgtcgagcg tcttctcctt ggccatggga agccctccag
    25321 tggttgaagc ccgattcacg ccatgcgtga tcgcgcaacc aacgcgccgc gtcgggcgac
    25381 gttccccgtg ccgcggaaaa gtcgggacac cacgaaaagt cgggacacgg gaaaagtcgg
    25441 acacgaaaag tcgggacacg aaccgggaca cgaaaagtcg ggacacgaac cgacttaacc
    25501 ctccggccga cggcgttcct cgcccgcgcc ttccgacccg gctgcgacac ccgctcaacc
    25561 cggcccattc cgcccccaga actgcccgat cgccgccgcc gcctcccggt cgatctcgtc
    25621 gctctccccc gtcgcccggc cgatgacgcc cgaaatgccg ccgccgaacc gcacctccat
    25681 gttggcggcg ttggcgatgg cgtcgcagcc gtggtcggcg acgcgggtga tcgagccctg
    25741 gacgagcgtg gtgccccgga cgctgccggc ggcgagggcc gtggcgaaga gcgcccggtc
    25801 ctcgtcgcca tgggccacga tcacggcccc gacctcgccc cggtgcgtgt ggcaggcggc
    25861 gacgatcagc gcggcgagct cggccttctc gatggccggg tcgatgcggc cggcgaagac
    25921 gccgccggcg atgaagggca gggcgaggcg ccggcagcca tgcgcggcgg cgagggtgaa
    25981 ggcgtttcgg atcgacgcct cgacggaggc ccgggtaggg tcgaagcccg gccggccctg
    26041 gagggtcatc gagcccgggg ccgcgtgggc gatcccccgg atcccctgcg cccggagccg
    26101 gccggccccg gtccacaagg cggccccgag cggcaccatg cgcagcccgc gcagctcacc
    26161 gttcaccggc gtcccggcgc aggcgacccg ctccgtcgcg acatatccca tccgcacctc
    26221 cgccgccccg ctcggggcca tcacgaatcg ttatgcccgc cgcccgccgc gccgggcaaa
    26281 gctgccttcg ggccggcaag cgaacccggg agcacgccag atgtcgcgga atacgcgaca
    26341 cctgcgctcg ggttgtcgcg tattccgcga caggcgcggc cggactgtcg cagattctgc
    26401 gacataagca ttcgtgatgt cgcggattct gcgacatccg acccgagaag tcgcagattc
    26461 cgcgacagag cccaacgaac gaaccgaagg attcgacgat cgaacccgcg gaatcgccga
    26521 ttccgcgggt tcgatcgtcg gtctcgcggg ttcgttcgcg gccagaaagc tacaggattc
    26581 ctgccttctg cctcgccccg accccgcaac ccttgacgga tcgcgccaga taggctataa
    26641 atcctatagc gctgcgatct cgatccgccg gccttcctcg gccgagcgga gcagggcgtc
    26701 gatcagccgg tggacgtgca gcgcgtccct ggcggtgacc cggggtgcgc tgccttggtc
    26761 cagggcgtcg aggaagtcgg tgatgaccgc cttgtgccat tcgtggctga acgccatggg
    26821 gtccgccccg ccgccgatgc cgccggcctc gcccgtggtt tcgctccggc catcgaggaa
    26881 gtcgagcgtg agccggccgc cgtcgagcac ggcgacgccc ttcgtccccg ccagcacgag
    26941 ctgctcggcg cggcccggat ggaaggtggt ggtcgccatg acggcaccca gcgccccgtt
    27001 ggcgaaggcg atgccgccgc ccatgaagtc ctcgccctcc atccggtgca tggccgaggt
    27061 gccgcccatg gcctgcaccg ctgctgccgg gccggtcagg ctgagcatca gatcgaggct
    27121 gtggatcgcc tgggtgatca ggaccccgcc gccgtcgcgc gccagggtgc cgcgcccggg
    27181 cacgtcgtag tagctctgcg gccgccacca ggggacgctg agctgcacgg tggcgagcgt
    27241 gccgagttcg ccatcggcca ggcgccgcgc cagctctgtg gcggcctccc ggaagcggtg
    27301 ctgcaggacc acgccggtgg tcaccccggc cgcctcggtc agcgccacca cccgctccgc
    27361 cgcctcgagc gtgcgctcga gcggcttctc catgagcagg tgcttgccct tgcccgcgag
    27421 ctgtcccacc agctccagcc gcgcgtcggg cggggtcagg atcatcaccg ctgtcacggc
    27481 cgggtcgtcg gcgatggcct cgagctcgtc gacgaccggc aggtcgtagc gctcctcgaa
    27541 cgcccgccgc cgctcggccg aggggctcca ggcccgcacc tcgacgcggc cctggaggtc
    27601 ctcgaggctc tgcgcgtgcg gggccaccgc catgccgagg ccgatgaggc cgatggttcg
    27661 cctggtcatg tctcctgctc tccctgccag tgaaggatgt cgatgggctg ctcgcgtccg
    27721 cgcaccgcgt gggtcggcag cgccagccag tggtccccct cgccggccgc ggcgacgagg
    27781 gccgccgagg cgacgaggcc gccatcggtc tcgcgggtga tctgctcgag ccgggccgcc
    27841 acgttgaccg tgtcgcccag caccgtgaac tcgagccggc tgctgtcgcc gaccgccccg
    27901 gcgaaggccg ggccgaaatg ggcaccgatg ccgatccgca ccggcgcctc gccggcggcg
    27961 gtcagccggt cgctccaggc cgcgacccgg cgcaggagat ggcgggcgca gtcgagcgtg
    28021 cggcgggcgt cgtcggcatg cccctcgggc accccgaaga ccacgatggc gctgtcgccg
    28081 acgaacttgt cgacgactcc ccgatgcgtc agggccgcct tgacgacatg atgccggaag
    28141 gtgccgagga aggtcgagag ctcgggcggg gccagatgct cgctgcggct ggtgaagccc
    28201 ctgatgtcga ccaccaggat gccgatccgc acgtcccagc cgcgccgcag cgcctcgaca
    28261 tcgccggcgg cgagcagggc cgcgatctcg ggcggcagat agcgggcgag gttggtgcgc
    28321 tccaccgcct cctcgatggc c
//
LOCUS       DAOTFI010000022        28046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1932_length_28046_cov_6.239613, whole genome shotgun sequence.
ACCESSION   DAOTFI010000022 DAOTFI010000000
VERSION     DAOTFI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 28046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28046
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1932_length_28046_cov_6.239613"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="P5558_03515"
     CDS             <1..949
                     /locus_tag="P5558_03515"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY23411.1"
                     /translation="AAVSAVLQPELRRRHYEIDFAAALIAASATLAQIIPPSLMLVII
                     AIQNNLSVGALFLGGVLPGLIACVLLMVVAFLHARTRPELAASLETFSLRRVAASFVR
                     AIPAMGLGVVILGGIIGGFMTPTEAAGVAVVYATAIGFLVYRELRLAELPRLLTRAAY
                     FSAGLLLLVAAANVLNWLVTTSGDLERLAGALGAVLQTKLTFLLGVNVVLMLLGMFIE
                     GIALILLLGGLLIELGTALGIDPHQMALIIVFNCAVGLITPPFGATLFVTSMVAERPF
                     VAVARRAFMPWLALTTALAAVSIFPELTLALPRLSGFLG"
     gene            965..1867
                     /locus_tag="P5558_03520"
     CDS             965..1867
                     /locus_tag="P5558_03520"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY23412.1"
                     /translation="MDRHSIDWNGPIPAVVTPFAEDRSIDEAAFAQNVENVLGSGAHG
                     IVVAGCTGEFWSLADAERARLFRLATEVTGERGFVIAGISAIEVDRVVSLAREARAAG
                     CDGALVLPPWFVQLNDDDITAHFTAVAKAVDLPLMVYNIPANAVNRIGPALASRLAGI
                     ETVVAIKESSGDWLNYYETFLAVQDRLRVFCGPSHLYGAQAALMGCDGVIDIFPNVWL
                     GGCNRIFHAAKAGRLDEAFALQEQGFRLSRAFTAEAMSLYVATKHAMNVLGLPGGAVR
                     PPFHPLTGHQAAFLEERMRELGLT"
     gene            2171..2590
                     /locus_tag="P5558_03525"
     CDS             2171..2590
                     /locus_tag="P5558_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY23413.1"
                     /translation="MLYATGHPDPLALMRSMMRDFQTQHTPRAQAAFPAVNVWQGEDA
                     VALTAELPGVEPADIDISVKDKVLTISGERRPPELPEAATWHRRERGYGKFSRAVQLP
                     FTVDESRVEARFQNGILKIAVGRPDADKPRKIEIKAA"
     gene            2605..3003
                     /locus_tag="P5558_03530"
     CDS             2605..3003
                     /locus_tag="P5558_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY23414.1"
                     /translation="MANQVTRTTDKTPADAPETTYGGRIYRPITDIVETGDGVTLMLE
                     MPGVAAEDVDVTLEKRVLTIQGKVRPTSIDRLNLAYAEYGEGDFERVFTMSDDFDPEK
                     IEAEVQNGVLTLRLPRAAAAQPRKITVKAA"
     gene            3029..3532
                     /locus_tag="P5558_03535"
     CDS             3029..3532
                     /locus_tag="P5558_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY23415.1"
                     /translation="MQIKDLIPWARKDHVPDAKSADDHPVTALQREMNRVFESFWSRF
                     GQGVSALDWPWGGSEVKSDVVETGDAVEVSIELPGMDMKDINVTVANDMLTIKGEKKV
                     ERQEEKKGYYLSERSYGAIYRTIPLPPGVDGEKAEAAFKNGVLTIKLPQTPEAQARIK
                     HIEVRSG"
     gene            complement(3580..3816)
                     /locus_tag="P5558_03540"
     CDS             complement(3580..3816)
                     /locus_tag="P5558_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23416.1"
                     /translation="MSETNRSFFERMELALAQVRPRLKGDDPGLVMLRAEHDVAAERL
                     KVARRAGSEVSDEALRSVRLAVERLLQHVGRRAA"
     gene            4030..4521
                     /locus_tag="P5558_03545"
     CDS             4030..4521
                     /locus_tag="P5558_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRY23417.1"
                     /translation="MRRWIVMLGLVLGACTGGLGNAPTYPLEVQIADLRFAEPGLLEQ
                     SIGLDLRFTNPNPEPISASGLRFSLALDGEAFGTGVSDAAIEIPRLGETIVPVTLRVQ
                     TGELINRVLGFDGNNIAYRLTGDLFRTGGLTGLAGGSLPFASESAIAIPDFQSLLGRL
                     SRG"
     gene            complement(4564..4899)
                     /locus_tag="P5558_03550"
     CDS             complement(4564..4899)
                     /locus_tag="P5558_03550"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HRY23418.1"
                     /translation="MPTFITTGRLTQDAVKGLAAKPEDRSEAVGKLIAAAGGKLLHYY
                     VTTGDTDFLLISEAPGADVAVAACMAAAASGAVSDVRTVQAWSSAEFKAVAAKAGQVI
                     AAYRAPGKS"
     gene            complement(5432..6364)
                     /locus_tag="P5558_03555"
     CDS             complement(5432..6364)
                     /locus_tag="P5558_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRY23419.1"
                     /translation="MSVFLDIVLPVFVLLGLGYAAAALDLFDAAATRALSRFVFQFAL
                     PVMLFSSLAAQGKPAGFAPAYLVAFFAGAAVCALLTALIAPRLGPIERRGRAIAGFSS
                     AYGNVVLIGIPLVLTAFGEAASLPLFLLVTFHGPIMMTLVTVLLEAGGDGGERRSLLI
                     GTLKGIAANPILLGLVGGILFGATGLELPKTIAASAELLGRAALPTALFAMGASLRGY
                     RIRGALPLAFTMVGLKLVLHPFIVWLLVDHVFRLDSPWSDVAIVLAAMPTGINAYLFA
                     ARYQSGEAEAASAVLISTALSLVSVSVILTLRLG"
     gene            6473..6661
                     /locus_tag="P5558_03560"
     CDS             6473..6661
                     /locus_tag="P5558_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23420.1"
                     /translation="MQETMPNAELVLHAGRREAEAVEYATDPDDERWAPLHCTLFITG
                     SALACWGVLITLVALVLG"
     gene            6877..7851
                     /locus_tag="P5558_03565"
     CDS             6877..7851
                     /locus_tag="P5558_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23421.1"
                     /translation="MDIASILANELGRRLAGNYSAIYGSLQPEYADILRTMAHVVIER
                     IADSDALYHDTDHTILVTMVGQAILKGRILVEPVTAEDWLHFTIATLVHDIGYLRGLC
                     PGDGGGRYVTDTAGSTIMLPEGASDAALGPYHVDRGKVFVRQRLSTVPAIDEERIARN
                     IELTRFPVPADGDHENTADEPGLVRAADLIGQLADPYYPRKWNALFHEFIEIGVAQRL
                     GYTSPADLARDYPRFFWSCVEPHVQPAIRHLDRTQEGKAWLAQLYSHVFVEEHMRRRT
                     GPERAHNRVTQEGGSARMPARQRARRAVDARPGRAGDRPPKAGSERDD"
     gene            7844..8263
                     /locus_tag="P5558_03570"
     CDS             7844..8263
                     /locus_tag="P5558_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY23422.1"
                     /translation="MTSHAPRRLLETALYAADLDTAEEFYGGLLGLEKVSRAGDRHVF
                     YRCGQGMLLVFNPEETRTPSRGPLPVPEHGTSGAGHACFAASAAEIDGWRRVFLAHGI
                     AIDADFRWPNGARSLYVRDPAGNSIEFAEPRLWELAD"
     gene            8891..9154
                     /locus_tag="P5558_03575"
     CDS             8891..9154
                     /locus_tag="P5558_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23423.1"
                     /translation="MRNMIILSAVVIMVGAPAAMAEDNDALLQASYQGAVTGDAEKDT
                     VAYDLRQPSDLWVRFSPECFAGAAAGDFARNAPTTAEIHQAAK"
     gene            complement(9218..9964)
                     /locus_tag="P5558_03580"
     CDS             complement(9218..9964)
                     /locus_tag="P5558_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23424.1"
                     /translation="MRALFIILLLASGVGVANAQSPLAEANWFIDAGWMALRDGEGFE
                     HAGELSKARAAYEYASHAANRALVIGDQVGVPLDARPASPYFLYGQAELHRAQLLHGL
                     GAPWPEIDHSLHQARQAFDDVLRLVDRRFPPGSPDWVGMRSEALFSLSTVYFLLNDLE
                     TADLVIRDLEALQPNLAPARELQGVIDYSTGQPTSFEPPPSVSPPEPAGSGIGSERFL
                     GYAIEIGKALFGRWGTVAGMLVEDVYRVVQ"
     gene            10066..10704
                     /locus_tag="P5558_03585"
     CDS             10066..10704
                     /locus_tag="P5558_03585"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRY23425.1"
                     /translation="MTNTLVLVRHGQSEWNARNLFTGWRDPELTAPGRLEAERAGRLL
                     RDRGLHFDIAFTSVLRRAEETSRIVLAELGQRDLPVVRDAALNERDYGDLAGLNKDDA
                     RKRWGDEQVHAWRRSFAVRPPGGESLQDTAARVLPYYERQILPRVLKGACVLVSAHGN
                     SLRALVMMLDGLEEAEVAALDIPTGVPLLYELDGQGRVLRKETLGGPDDPRR"
     gene            complement(10812..12155)
                     /locus_tag="P5558_03590"
     CDS             complement(10812..12155)
                     /locus_tag="P5558_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRY23426.1"
                     /translation="MIAGFITASGILIAASQLKHILGVPAGGSNLVAIAGSLIRNLPA
                     THPWTLAIGLSAMAFLFWVRRGLKPLLVGLGLRPRLADTLAKAGPIIAVAVTIALSSA
                     LSLEERGVRIVGAVPQGLPPFALPGLDLELLGRLLVPAILISVIGFVESVSVASTLAA
                     KRRQRIDPDQELVGLGAANLASAVSAGYPVTGGFARSVVNFDAGAETPAAGAFTAVGI
                     AFATLFLTPLLFFLPQATLAATIIVAVLSLVDLGVLKRTWTYAKADFAAVAATILLTL
                     GAGVEIGVTTGVLVSLVLHLYRTSRPHLAVVGRVPGSEHFRNVLRHEVETDPAVLSLR
                     VDESLYFANARYLEDRLYDMVAARPELRHVVLMCPAVNAIDASALESLEEINRRLADS
                     GVTFHLSEVKGPVMDRLQRVDFLDHLTGRVFLSQHQAMTELSGAHRARSDRATAK"
     gene            complement(12152..13108)
                     /locus_tag="P5558_03595"
     CDS             complement(12152..13108)
                     /locus_tag="P5558_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRY23427.1"
                     /translation="MTDLQRTGAAPNGNHRPSGLARYFPILDWGRTYGRGAATGDLVA
                     ALIVTIMLIPQSLAYAMLAGLPPEVGLYASILPLVAYAVFGTSRSLAVGPVAVVSLMT
                     AAAVGEIAAAGTADHATAATALALLSGLLLVGLGLLRLGFIANFLSHPVIAGFITASG
                     ILIAASQLKHILGVPAGGSNLVAIAGSLIRNLPATHPWTLAIGLSAMAFLFWVRRGLK
                     PLLVGLGLRPRLADTLAKAGPIIAVAVTIALSSALSLEERGVRIVGAVPQGLPPFALP
                     GLDLELLGRLLVPAILISVIGFVESVSGCSAWASSPTSSAIR"
     gene            complement(13147..13380)
                     /locus_tag="P5558_03600"
                     /pseudo
     CDS             complement(13147..13380)
                     /locus_tag="P5558_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
     gene            complement(13600..14505)
                     /locus_tag="P5558_03605"
     CDS             complement(13600..14505)
                     /locus_tag="P5558_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY23428.1"
                     /translation="MSSVTNSFVPKIDLDVRPEVTAFFDPATNTISYVVRDPASTACA
                     VVDSVMDIDYAAGRITFDHADAIIAFVRREDLQVEWLIETHVHADHLSAAPYIQRELG
                     GRLGIGENITVVQETFGKVFNEGTEFRRDGSQFDRLFKDGDSYTIGTMPAVAIHTPGH
                     TPACMTHIVGDAAFVGDTLFMPDGGTARADFPGGDARTLYRSIRRLLELPDVMRLFMC
                     HDYGPNGRDIAWETTVGEERQHNIHVRDGTTEDEFVAKREARDRTLDMPKLIIPSLQV
                     NMKAGRLPEPDASGKRFLKVPLNTL"
     gene            14883..15860
                     /locus_tag="P5558_03610"
     CDS             14883..15860
                     /locus_tag="P5558_03610"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRY23429.1"
                     /translation="MARLSAYRVPSTWRSLFELAVTFGPFVVAWTLLAIAVHFDQLWL
                     YALLLLPAGGLLVRLFMIQHDCGHGSFLRRRWSNDWIGRAIGIVTLTPYDHWRQAHAV
                     HHATTGNLDQRGVGDIDTLTVREYRALPWRRRLLYRLYRNPLVMFGIGPFYMFVLQNR
                     LPLGAMRKGWMPWVSTMATNAAIVAAAALLIWAMGLGPFLLVHLPVVALGATAGVWLF
                     YVQHQYEGTLWDKGDDWNAHEAALHGSSHYDLPAILRWFTGNIGIHHVHHLSSRIPFY
                     RLREVLRDHPELREVGRLTLWQSFRCVRLALWDEEKRRMVTFREAAASA"
     gene            complement(15868..16677)
                     /locus_tag="P5558_03615"
     CDS             complement(15868..16677)
                     /locus_tag="P5558_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRY23430.1"
                     /translation="MRIHLGCELTYEVPNPTPMIVLLNVHHSCVARLERPDWLMTTPA
                     VPIESYRDGFGNWCCRLVAPSGRFVLGTDSVIRDSGESDPVALDAMQHPVEHLPPDSL
                     QFLLGSRYCETDCLSREAWRLFGQGPTGWARVQAICDFVNRHITFGYEHSRSTRTAAE
                     AYAEGRGVCRDFTHLAIAFCRCMNLPARYCTGYVSDIGQPPPYASMDFAAWMEVYLGG
                     SWWVFDPRNNRPMYGRIKIAQGRDAADVPLTHTFGPNFLAGFRVWTDEIPA"
     gene            16776..17723
                     /locus_tag="P5558_03620"
     CDS             16776..17723
                     /locus_tag="P5558_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRY23431.1"
                     /translation="MSHDHAGHSHAAPATEGRLALAVLVNVGLTVVQVVGGILSGSLA
                     LVADALHNFSDAASLGIAWGARRIARRPADASMTFGYARAEIVAALINFTTLIILGLY
                     LAYEAVLRLLDPQPILGWTVVVIAAIALVIDLVTAFLTYAGSKDSMNIRAAFLHNVAD
                     ALGSVAVIFAGTLVLLYDWTIVDPLVTLAIAAYVLWHGGTEIGGAIRILMNAAPDGVD
                     TQEVAGAMTATAGVADVHHVHLWRLDERRTSLEAHVALDADAARHPAAVKARLRTMLT
                     ERFGVGHVTIETELPGEACAAPGGVQPQGGRPAAGVAAD"
     gene            complement(17720..18676)
                     /locus_tag="P5558_03625"
     CDS             complement(17720..18676)
                     /locus_tag="P5558_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRY23432.1"
                     /translation="MLASLILGFVLLVVGGESLVRGAVATATRLGLSPLFIGIVLVGF
                     GTSTPELVTSVQAALAGSPGIAVGNIVGSNIANILLILGVGALLLPMACHPGAFYRDG
                     TVLVLSAAALGAVVLVGALDRWTGLAFVAALLAYVAFTYVRESDSPDEAAQMHEAEAA
                     LQLTEQPMPLWLALLIALAGIALTIVGARLLVSGAIELATAMGVSETLIGLTVVAVGT
                     SLPELVTAVIAALKKEADVAFGNIVGSNIYNTLGILGITATVQPLDVPREIADFDIWV
                     MLAATVLLLFFAISGWRVSRREGAVLLALYAAYVGYLAWDLF"
     gene            18763..19506
                     /locus_tag="P5558_03630"
     CDS             18763..19506
                     /locus_tag="P5558_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY23433.1"
                     /translation="MWQAENLALVALTFLVAGFVKGIVGLGLPTIVLGVLTTTYGITP
                     AIAIMLVPSLVTNAIQGLGGQTSLAVLRRLWPLLLAAAVGIVVGAGVLARSSGSGPAV
                     ALGLLIMLHGCISLLGPRLPHPGPHERWLSPLAGLANGLITGFTGSAILPGMLYIQAL
                     GFDKERLVQAMGLLFGISTLVLMVAFARYELLTPDLALLSALALLPALLGLRLGARLR
                     RRIAEERFRRLLYVVLIALGGAIVWRNIG"
     gene            complement(19511..20632)
                     /locus_tag="P5558_03635"
     CDS             complement(19511..20632)
                     /locus_tag="P5558_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HRY23434.1"
                     /translation="MGERIGVMVCGHGSRSTAAVAEFAAVAQKLEGAFPDWPVSYGYL
                     EFANPVIRDGLDRLREAGCTRVLAVPGMLFAAGHAKNDIPSVLNTYQKAHGLRIDYGR
                     ELAVDARMIRAAADRIEAALARADAEHGAMPRHKVCLVVIGRGASDPDANSNVAKIAR
                     MLWEGMGFGWCETGYSGVTFPLVEPCLEHVAKLGYKRVVVFPYFLFTGILVDRIFGFT
                     REVAARHPSIRFVEAGYLGDHPLVVETMAARVREILEGANLMNCSLCKYRAQVLGFEA
                     EVGLPQASHHHHVEGKGASAPGSSVADCPLCDDFCTGACRLVDAHAHDHGHEHAPGHE
                     HDHHHGPDHHHGHDDHHHHPYPHAHHPLGPVSALNRRRR"
     gene            20795..22111
                     /locus_tag="P5558_03640"
     CDS             20795..22111
                     /locus_tag="P5558_03640"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY23435.1"
                     /translation="MALKTQIVVVGGGAGGLELVARLGARHGRDGRFDIILVEEKRTH
                     IWKPLLHEVATGALDANLDEVGYRAHAYRWGYRYFMGRLEAIDRAAGQIVIAPILDED
                     GKEVMGRHLIRYDFLVLALGSVTNDFGTPGVAEHCIFLDQRTAADRFRQKLLDHCLRV
                     SRTLSMDPRADAHVDVAIVGGGATGVELAAELYNAAAALRHYGLEVFDESRLRVALLE
                     AGPRLLPALPEKLAQAARDELESLGVRVLVETRVTEVTNAAMRTHTGEVISADLRVWA
                     AGIKGPDLLSDIGGLETNRICQLIVRPTLQTTRDARIFAFGDCCSCTPEGAERPIPPR
                     AQAAHQMARTVFRNLQHLMQNRRLEHFVYRDHGSLVSLSRFSTVGSLMGNLIGGRMAI
                     EGRLARFVYASLYWKHLLTIHGWLRGIAHIVVGQVNHVIRPRLKLH"
     gene            22111..22743
                     /locus_tag="P5558_03645"
     CDS             22111..22743
                     /locus_tag="P5558_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HRY23436.1"
                     /translation="MDRTSDPRRHAPATLRNRAPILAVLQRVLPASGLLLEVASGSGE
                     HAAWMAPQLPPGLAWQPSEADALALPGIDAHARDAGATTVRPAIRLDAAAADWPIAAA
                     DAVFCCNLAHIAPWAATEGLLAGAARLLPPGGPLVLYGPFRRHGRHTAASNAAFEASL
                     QARDPAWRIRCVDNELVPAAARHGLVLDEVAELPANNLVLVWRRESLSAA"
     gene            complement(22730..23647)
                     /locus_tag="P5558_03650"
     CDS             complement(22730..23647)
                     /locus_tag="P5558_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HRY23437.1"
                     /translation="MSRTLTVAAAQLGPIARGDSRARVVGRLIDLLRQAKARGAGFVV
                     FPELALTTFFPRWGLAEPDEVDAFFEREMPGPETAPLFAAAGELGLGFALGYAELVTD
                     AEGRARHFNTAILVEPDGRISGKYRKIHLPGHREVEAWRPFQHLEKRYFEPGDLGFPV
                     FDTMGGRLGMLICNDRRWPEAFRMLGLQGVELVAIGYNTPQHYPLVPEQDHLQQFHNR
                     LVLQAGAYQNGTWVVGVAKCGVEEGCMLIGESCIVAPTGEVVALCSTLEDEICVAACD
                     LDRCQSIKANIFDFKAHRMPAEYGLLTQR"
     gene            complement(23644..24852)
                     /locus_tag="P5558_03655"
     CDS             complement(23644..24852)
                     /locus_tag="P5558_03655"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HRY23438.1"
                     /translation="MSRFARPAHIANPAVLRHFPYPPALEPILAAADLEAVRAEITSW
                     PGYAPTPLHDLRAMAEAAGVAAIHYKDEATRFGLASFKALGGAYAVRRLLERLFVERE
                     RRPARDAAELARFARSVTVTCATDGNHGRSVAWGARRFGARCRIYIHATVSQGRKRAI
                     EAFGAEVVRTPGNYDDSVRRAAEDAARHGWHVVSDTSYPGYTEVPADVMRGYLLMADE
                     AFAQLAGAPMPTHVLVQGGVGGVAAAVAARSWQILGPDRPRFIVVEPERAACIAASIE
                     AGERRAVAGDLDTLMAGLACGEVSLVAWEVLRLAVHDVLVIDDAAAVTVMRDLARNDP
                     PVVAGESATCGLAGALAALEYPEIAELLGLGPASRLLVFGTEGATDPELYARLVGAEP
                     VGRPAEEIAP"
     gene            25197..26114
                     /gene="cysD"
                     /locus_tag="P5558_03660"
     CDS             25197..26114
                     /gene="cysD"
                     /locus_tag="P5558_03660"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035683265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRY23439.1"
                     /translation="MPRRSATLSSHLRALEAESIFIMREVAAEMANPVLLYSIGKDSS
                     VMLHLAMKAFWPAKPPFPLMHIATGWDFRDMIVHRDQLTRALGLRLLVQSNETATAEG
                     STPFNTDSREWARIMLIDALKAGMDRHGFDAAFGGGRRDEEKSRAKERVFSVRSAGHG
                     WDPRNQRPELWHLYNTRVARGETIRVFPLSNWTEFDIWEYIRLEQIPVVPLYFAKRRP
                     MVDRGGTLIMVDDERMQFQPGETASERLVRFRTLGDWPLSGAIESDADTVEQVIAEML
                     SARTSERQGRLVDSDEAASMEKKKREGYF"
     gene            26114..27997
                     /gene="cysN"
                     /locus_tag="P5558_03665"
     CDS             26114..27997
                     /gene="cysN"
                     /locus_tag="P5558_03665"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HRY23440.1"
                     /translation="MTRQAEAVAIATRAEEAAKGLLRFLTCGSVDDGKSTLIGRLLYD
                     SKLIMEDQLRSLADDSKKHGTVGEDIDLALLVDGLEAERQQGITIDVAYRFFSTARRS
                     FIVADTPGHEQYSRNMATGASTCDLAIILIDARKGVLTQTLRHSYLVHLLGIKHVVLA
                     VNKMDLVGFAEGRFAEIVADYRAATSQLAFRDVLPIPLSARFGDNVTMPSNRMPWYRG
                     PMLLEHLEAADVTAEAGRSAFALPVQWVNRPHQDFRGFAGTIVSGRIAAGDPVVVAKS
                     GRATRVSRIVTADGDRAEASTGQAVTLVLDEEIDIARGDVLVEPRARPEVADQFAAHL
                     FWMHEEPLLPGRRYLLKLGAELVPATITSIRHKIDVNTQEERPGRTLELNEIAFCNLH
                     TTQPIVFTAYTENRDLGGFILIDRTTNQTVGAGMVEFGLRRASNIHHQHLDIDKARRA
                     AQKGQKPCLVWFTGLSGAGKSTIANILERKLVAQGAHTYLLDGDNVRHGLNKDLGFTD
                     ADRVENIRRVGEVAKLMVDAGLIVLASFISPFRDERRMVRDLLGEGEFIEVFVDTPLA
                     IAETRDTKGLYKKARAGQIKHFTGIDSPYEPPEQAEVTVATGELSAEAAADQILAYLT
                     SHL"
BASE COUNT         4487 a         9826 c         9587 g         4146 t
ORIGIN      
        1 cgcggccgtc tccgccgtgc tccagcccga gctgcgccgc cggcactacg agatcgactt
       61 cgccgcagcc ctcatcgccg cctcggcgac cctcgcccag atcatcccgc ccagcctgat
      121 gctggtcatc atcgccatcc agaacaacct ctcggtcggc gccctcttcc tcggcggcgt
      181 gctgccgggc ctgatcgcct gcgtgctgct gatggtcgtg gccttcctcc acgcccgcac
      241 ccggcccgag ctggcggcca gtctggagac cttctcgctc cgccgggtgg ccgcctcctt
      301 cgtccgggcg atcccggcca tggggctcgg cgtggtgatc ctcggcggca tcatcggcgg
      361 cttcatgacg ccgaccgagg ccgccggcgt cgccgtggtc tatgccacgg cgatcggctt
      421 tctcgtctat cgcgagctgc gcctcgccga gctgccgagg ctgctcacgc gggccgccta
      481 tttctcggcc ggccttctcc tgctcgtcgc cgcggccaac gtcctgaact ggctcgtgac
      541 cacctccggc gacctcgagc ggctggcggg tgctctgggt gcggtgctgc agacgaagct
      601 aacctttctc ctcggcgtca acgtggtcct catgctgctc ggcatgttca tcgagggcat
      661 tgccctcatc ctcctgctcg gcggcctcct catcgagctc ggcaccgccc tcggcatcga
      721 cccgcaccag atggcgctga tcatcgtctt caactgcgcc gtcgggctga tcacgccgcc
      781 cttcggcgcc acgctcttcg tgacatccat ggtggccgag cgaccgttcg tcgccgtcgc
      841 ccgccgggcc ttcatgccct ggctggcgct caccacagca ctcgccgccg tctccatctt
      901 tcccgagctg acgctcgccc tgccgcggct ctcgggcttt ctcggctgaa cggagaatat
      961 cgtcatggac aggcacagca tcgactggaa cggcccgatc ccggccgtgg tcaccccgtt
     1021 cgccgaggac cggagcatcg acgaggcggc tttcgcccaa aacgtcgaga acgtgctggg
     1081 atccggggcg catggcatcg tcgtcgccgg ctgcaccggc gagttctggt ccctcgccga
     1141 tgccgagcgc gcgcggctct tccgcctcgc gaccgaggtc acaggcgagc gcggcttcgt
     1201 catcgccggg atcagcgcca tcgaggtcga ccgggtcgtc agcctggcgc gggaggcgcg
     1261 cgccgccggc tgcgacgggg cgctcgtgct gccgccctgg ttcgtccagc tcaacgacga
     1321 cgacatcacc gcccacttca ccgcggtggc gaaggccgtc gacctgccgc tcatggtcta
     1381 caacatcccg gccaacgcgg tgaaccgcat cgggccggcc ctggccagcc gcctcgccgg
     1441 gatcgagacg gtggtcgcga tcaaggagag ctccggcgac tggctgaact actacgagac
     1501 gttcctcgcc gtgcaggacc ggcttcgcgt gttctgcggc ccgtcacacc tctacggcgc
     1561 ccaggccgcg ctgatgggct gcgacggcgt gatcgacatc ttccccaatg tctggctcgg
     1621 cggctgcaac cggatcttcc acgcggcgaa agccggccgg ctcgacgagg ccttcgccct
     1681 gcaggagcag ggctttcgcc tctcccgcgc cttcacggcg gaggccatgt cgctctacgt
     1741 cgccaccaag cacgccatga acgtgctggg cctgcccggc ggcgccgtcc gcccgccttt
     1801 ccatccgctg accgggcacc aggcagcctt cctggaggag cggatgcgcg agctcggcct
     1861 gacctgagcg agccagggaa tcccggtcgc gacagccgcc gtttttccgt catcgccccc
     1921 ttgaacggcc gatttccgct gcctttatct atctcgaaac gcccgcgaat ggggtttccg
     1981 gaacacgcga caggttcggc tttggagcgg accgccgatc gttcgccgca agcgggaacg
     2041 aggccggagt cgtaggaaag ttcgctcgcc gcgcgctcgc ggcgccacct gaagctcgtt
     2101 cgtcccccgc gggcgtctcc gccgatgcat ggcaccggcg aaggaaggtc gctgacgaag
     2161 gagacgagca atgctctatg ccaccggaca ccccgacccg ctggcgctca tgcggtccat
     2221 gatgcgcgat ttccagacgc agcacacgcc ccgggcgcag gccgcgttcc cggccgtcaa
     2281 cgtctggcag ggcgaggacg cggtcgccct caccgcggag ctgcccggcg tcgagcccgc
     2341 cgatatcgac atcagcgtca aggacaaggt cctgacgatc agcggcgagc gccgcccccc
     2401 cgaactgccc gaagcggcca cctggcaccg gcgcgagcgc ggctacggca agttcagccg
     2461 ggccgtccag ctaccgttca ccgtcgacga gagccgggtc gaggcacgct tccagaacgg
     2521 catcctgaag atcgccgtcg ggcgcccgga cgcggacaaa ccccgcaaga tcgagatcaa
     2581 ggcagcctga gaggaggacc gaagatggcc aaccaggtta ccaggaccac cgacaagacg
     2641 cctgcggatg cgccggagac cacctatggc gggcggatct accgcccgat cacggacatc
     2701 gtggaaacgg gcgacggcgt gacgctgatg ctggagatgc ccggtgtcgc ggccgaggac
     2761 gtggacgtca cgctcgagaa gcgcgtgctg acgatccagg gcaaggtcag gccgacgtcg
     2821 atcgaccggc tgaacctcgc ctatgccgag tacggggaag gcgatttcga gcgcgtcttc
     2881 accatgtcgg acgacttcga tcccgagaag atcgaggcgg aggtgcagaa cggggtgctg
     2941 accctgaggc tgccccgggc cgccgccgcc caacccagga agatcacggt aaaggccgcc
     3001 tgatgcaggc ggcaaaagga ggtgacccat gcagatcaag gatctgatcc cctgggcacg
     3061 caaggaccac gtgcccgacg cgaagtccgc cgacgatcat cccgtcaccg cgctgcagcg
     3121 ggagatgaac cgggtgttcg agagcttctg gagccgcttc ggccagggcg tcagcgcgct
     3181 cgactggccg tggggcggca gcgaggtgaa atcggacgtc gtcgagacgg gtgatgccgt
     3241 cgaggtgtcg atcgagctgc ccggcatgga catgaaggac atcaacgtga cggtggccaa
     3301 cgacatgttg accatcaagg gcgagaagaa ggtcgagcgg caggaggaga agaagggcta
     3361 ctacctgtcc gagcgcagct acggcgccat ctaccggacg atcccgctgc cgcccggcgt
     3421 ggacggcgag aaggcggaag ccgcgttcaa gaacggcgtg ctgaccatca agctgcccca
     3481 gaccccggag gcccaggcca ggatcaagca catcgaggtc aggagcggct gactccgagg
     3541 ccccggcgcc gtctcgcggt gccggggccc tcgttcccct tacgctgccc ggcgccccac
     3601 atgctgcagc agtcgctcga ccgcaagccg gacggaccgg agagcctcgt cgctgacctc
     3661 gctgccggca cgccgcgcca ctttcaggcg ctcggccgcc acgtcgtgct cggcccgcag
     3721 catgaccagg ccgggatcgt cccctttcag cctcggtcgc acctgcgcca gcgccagctc
     3781 catccgctcg aagaagctgc ggttcgtctc gctcatggct gctcctccct ccagtccacc
     3841 gaaacctgcc cagtctaccg aaacccgccc agtctaccga aacccgcgag gcggccatcc
     3901 taccatatga acgaggcctc cgctccatcc cgatcgacca atgccatcca aaggctcgcg
     3961 gtcgggggat ggggggtggt agaagcgccc gaccgggata gcggcggcaa ggtgggaggg
     4021 gcgatgatca tgcggcgctg gatcgtgatg ctgggcctcg tgctcggcgc ctgcacgggc
     4081 ggcctcggca acgccccgac ctacccgctc gaggtgcaga tcgccgatct gcgcttcgcc
     4141 gagcccggcc tgctcgagca gtcgatcggt ctcgatctcc gcttcaccaa ccccaatccc
     4201 gagccgatca gcgcctcggg cctgcgcttc tcgctggcgc tcgacggcga ggctttcggc
     4261 accggggtca gcgacgccgc gatcgagatc ccgcggctcg gcgagacgat cgtgccggtc
     4321 acgctgcgcg tccagaccgg cgagctgatc aaccgggtgc tcggcttcga cggcaacaac
     4381 atcgcctacc gcctcaccgg cgacctgttc cgcaccggcg ggctcaccgg cctcgccggc
     4441 ggcagcctgc ccttcgccag cgaatcggcc atcgccattc cagacttcca gagcctgctc
     4501 ggccgcctgt cgcgcggctg aggccggccc ggcggtcccg tcacagcgag gccggagcgg
     4561 cgctcagctc ttgccgggcg cccggtaggc cgcgatgacc tggcccgcct tggcggctac
     4621 cgccttgaac tcggcggagg accaggcctg gacggtccgg acgtcggaga ccgccccgga
     4681 ggccgccgcc gccatgcacg ccgcgaccgc cacatcggcc cccggcgcct cgctgatcag
     4741 caggaaatcg gtgtcgccgg tcgtgacata gtagtgcagc agcttgccgc cggcggcggc
     4801 gatcagcttg cccaccgcct cgctccggtc ctccggcttc gccgccagcc ccttgacggc
     4861 gtcctgcgtc agccgccccg tggtgatgaa tgtcggcatt ccattgctcc ctgtgattcg
     4921 agcttcttgc tgctcggacg ccgaagccta gcagattgcg acgcggcgcg atgcaaatga
     4981 gtgtgggcga ccactcgccc gccctgttgc atggaatgct tcaagaacct caggttggtg
     5041 cattttatgc aacaagaaac ccgagattgt tgcaatatct gcgacaatgg gcgtgggttg
     5101 tcgcggatat tgcaacacca ttatgacaaa tgtcgcgcta tccgcgacaa atcacgacga
     5161 acgaacccgc cgagccgacg agcgaaccca cggaatcgac gagcgaaccc gccaatcggg
     5221 cgaacgaacc cgggattcga cgaacgaacc cggcgcccga ccaacgaacc cggcatgctg
     5281 caacagcttg ccacagcacg ctttcatgcg ttcgggcagc gccccggcgg tcgctcggcg
     5341 gccgccaggg gcgaatttgc cgatcgaacc cgcgcccact tgcatcgccg gccttttcat
     5401 gataggatta ttttctgata gcaattgaca ctcagccaag gcggagcgtc aggatgaccg
     5461 agacggagac cagcgagagg gcggtcgaga tcaggacggc cgacgccgcc tcggcctcgc
     5521 cgctctggta gcgcgcggcg aagagatagg cattgatgcc ggtcggcatg gcggcgagca
     5581 cgatggcgac gtccgaccag ggcgaatcga ggcggaagac gtggtcgacg aggagccaga
     5641 cgatgaacgg gtgcaggacc agcttcaggc cgaccatggt gaaggcgagc ggcagggctc
     5701 cccggatgcg gtagccgcgg agcgaggcgc ccatggcgaa gagggccgtg ggcagggccg
     5761 cccggccgag cagctcggcg ctggccgcta tggtcttcgg caactcgagc ccggtggcgc
     5821 cgaagaggat gccgccgacg aggccgagga ggatcgggtt ggcggcgatg cccttgagcg
     5881 tgccgatgag gaggctccgg cgttcccccc cgtcgccgcc ggcctccagc agcacggtga
     5941 cgagggtcat catgatcggc ccgtgaaagg tgacgaggag gaagagcggc aggctcgccg
     6001 cctcgccgaa ggcggtgagc acgagcggga tgccgatcag gacgacattg ccataggcgc
     6061 tggagaagcc ggcgattgcc cgcccccgcc gctcgatcgg cccgagcctg ggcgcgatga
     6121 gcgccgtcag gagggcgcag accgcggcac ccgcgaagaa ggcgacgaga taggcggggg
     6181 cgaagccggc gggcttgccc tgcgccgcga ggctcgagaa gagcatgacc ggcagggcga
     6241 actggaagac gaagcgcgag agcgcccggg tggcggccgc gtcgaagagg tcgagcgccg
     6301 ccgcggcata gccgaggccg agcagcacga agaccggcag gacgatatcg aggaagacgc
     6361 tcaagccggc gagtccccgc ctcttaacgc aagcttagcg aatgcggcgt agccttcgat
     6421 cccgacgagg gccgtcaccc cgatgcccgg agtctcgagc ggagaccaag cgatgcagga
     6481 gaccatgccg aacgccgagc tcgttctgca cgccggccgc cgcgaggcgg aggcggtcga
     6541 atacgcgacc gacccggacg acgagcgctg ggcgcccctg cactgcacat tgttcatcac
     6601 cggctcggcc ctcgcctgct ggggcgtcct catcacgctc gtggccctgg tcctcggctg
     6661 accgcgtcga ccagacccgg ggcgccggct cgcacacctt agagccggca acgcctgacg
     6721 gccaagcgcc gagggctgcc gccaggcggc tggccgagcg gccggggcgc gagcctgcga
     6781 acggccacgc aggcccggct tgcccaagcc gcccggcttc ggcaatctgc atcctggcag
     6841 cggcgcttct cggcgcgccc tgccgggagg tgggtgatgg acatcgcgag catcctcgcg
     6901 aacgagctgg gccggcggct ggcgggaaac tattcggcga tctacggttc cctccagccg
     6961 gaatacgccg acatcctgcg gacgatggcc catgtggtga tcgagcggat cgccgacagc
     7021 gacgcgctct accacgacac cgaccacacc atcctcgtca ccatggtcgg ccaggcgatc
     7081 ctcaaggggc ggatcctggt cgagccggtg accgccgagg actggctgca tttcaccatc
     7141 gccacgctgg tccacgatat cggctatctg cgcggcctct gcccgggcga tggcgggggc
     7201 cgctacgtca ccgacaccgc cggatcgacc atcatgctgc ccgagggcgc ctcggacgcg
     7261 gcgctcgggc cctaccatgt cgatcgcggc aaggtcttcg tccgccagcg cctgtcgacc
     7321 gttcccgcca tcgacgagga gcggatcgcc cgcaacatcg agctcacgcg ctttccggtg
     7381 ccggccgacg gcgatcacga gaacaccgcc gacgagccgg gcctggtgcg cgccgccgac
     7441 ctgatcggcc agctcgccga tccctactac ccgcgcaaat ggaacgcgct cttccacgag
     7501 ttcatcgaga tcggcgtcgc ccagcggctc ggctacacca gcccggccga cctcgcccgc
     7561 gactatcccc gcttcttctg gtcctgcgtc gagccgcatg tgcagccggc gatccgccat
     7621 ctcgatcgca cccaggaggg caaggcctgg ctggcccagc tctacagcca tgtcttcgtc
     7681 gaggagcaca tgcgccggcg caccggcccg gagcgcgccc acaaccgggt gacgcaggag
     7741 ggcgggtccg ccaggatgcc agcccggcag cgcgccagac gggcggtcga tgcacggccg
     7801 ggccgtgccg gggaccggcc accgaaagcc ggcagcgaac gcgatgacta gccacgcacc
     7861 gcgccgcctg ctcgagacgg cactctacgc cgctgatctc gacaccgccg aggagttcta
     7921 cggcggcctg ctggggctcg agaaggtcag ccgggccggc gaccgtcatg tcttctatcg
     7981 ctgcgggcaa ggcatgctgc tcgtcttcaa tcccgaggag acgcgcaccc cgtcacgcgg
     8041 cccgttgccg gtgcccgagc acggcaccag cggcgccggc cacgcctgct tcgccgccag
     8101 tgccgccgag atcgacggct ggcgccgggt cttcctggcg cacggcatcg ccatcgacgc
     8161 cgacttccgc tggccgaacg gcgcccgctc gctctatgtg cgcgaccccg ccggcaactc
     8221 catcgagttc gccgagccgc gcctctggga gctggccgac tgagccggtc gtcgacgcag
     8281 cgtcggatgt cggggctcga ggctatgtac cggcagccgc attcggaccc ttccggcccg
     8341 cagcatcgcc accagaatgg accccgcgac gccttgccca agccacgaat ggaaaggcgc
     8401 gggttccgcc gccgatggca ttcggcactt gccatccgat aacgtatgcg ttattgaatc
     8461 acccgcggcg agatcgtcaa gagcaacaat aaacggcgtc gccagattgc atcgcggcat
     8521 ggtggcttgc tcactatgct gcgatgcaga tccgtttctt cccggttgcc tgtggcccga
     8581 gcgtcggtgc cggaagaccg gcgccgcaat ggccgtgccg tcgcggcgat cgccaccgca
     8641 tctcccccac ccatgcatcg aacggcggcc atcaagaaaa gccgatgttg tgcgattcgc
     8701 cacttactgg tccggcgcat cgtcccatcg tcattcgcga gccaaccggc agcatgctcg
     8761 aagatcagag cacgcaaacg gaaaggtgtc agtcgtcgcg ccgggcgtgt gatcgaagcg
     8821 cgacgagcgg accggaccac cggcgccgcg atggggtgcg ccgcaaccag caggaaagcg
     8881 agaagaaacc atgcgcaaca tgatcattct ttcggcagtc gtcatcatgg tcggtgctcc
     8941 ggccgccatg gccgaggaca acgacgccct gctccaggcc agctaccagg gtgccgtgac
     9001 cggcgacgcc gagaaggaca cggtggccta cgacctgcgc cagccatccg atctctgggt
     9061 ccgcttcagc cccgaatgct tcgcgggcgc cgccgccggc gacttcgcca ggaacgcccc
     9121 gacgacggcg gagatccatc aggccgcgaa gtagcatccg ccagcgggcg ccggcccgcc
     9181 ggcgcccgcg ccgacccggc gggcggtcga tgcctgccta ttgcacgacc ctgtagacat
     9241 cctcgaccaa catgcccgcg acggtgcccc agcggccgaa cagggccttg ccgatctcga
     9301 tggcatagcc gaggaagcgc tcgctgccga tgcccgatcc ggccggctcg ggtgggctca
     9361 cgctcggcgg cggctcgaat gaggtcggct ggccggtgct gtagtcgatg acaccctgca
     9421 gctcacgagc cggagcgagg ttcggctgca gcgcctcgag gtcacggatg acgagatcgg
     9481 ccgtttcgag gtcgttcagc aggaaataga cggtgctcag cgagaacagc gcctcgctgc
     9541 gcatgccgac ccagtcgggg ctgcccggcg gaaagcggcg atcgacgagc cgcaggacgt
     9601 cgtcgaaggc ctgccgcgcc tggtgcaggc tatgatcgat ctcgggccat ggggcgccca
     9661 ggccatgaag aagctgggca cggtgcagct cggcctgacc atagagaaag tacgggctcg
     9721 ccggccttgc gtcgagaggg acacccactt gatcgccgat aaccagagcc cgattggctg
     9781 catgcgaggc gtattcgtag gctgcccgag ctttcgagag ttcgccggca tgttcgaagc
     9841 cctcgccgtc acgcagcgcc atccagcccg cgtcgatgaa ccagttggcc tcggccaatg
     9901 gcgattgcgc gttcgcgacg ccgacgccag aggccagcag caggatgatg aataacgcgc
     9961 gcatcggact gctccctgtt gttcggctct cgactgtgat catgtctttg gccgactgcg
    10021 gtcgcaagct tccggcggcg gaaggggcgg cggagggaag gaacggtgac caacacgctg
    10081 gtgctcgtcc ggcatggaca gagcgaatgg aacgcgcgga acctgttcac cggctggcgc
    10141 gacccggagc tgacggcgcc gggtcgcctg gaggccgagc gggccgggcg cctgttgagg
    10201 gacaggggtc tgcacttcga catcgcgttc acgtcggtgc ttcgccgggc cgaggagacc
    10261 agccggattg tcctggcgga gctcggccag cgcgacctgc cagtggtccg ggatgcggcg
    10321 ctgaacgagc gcgactacgg ggaccttgcc ggcctgaaca aggacgatgc ccgcaagcgg
    10381 tggggcgacg agcaggtcca tgcgtggcgg cgttcctttg cggttcgccc gcccggcggc
    10441 gaaagcctgc aggatacggc ggcgcgcgtc ctcccctatt acgagcggca gatcctgccg
    10501 cgcgtcctga agggcgcatg cgtgctggtg tcggcccacg gcaacagcct gcgggcgctc
    10561 gtgatgatgc tggacgggct ggaggaagcc gaggtggcgg cgctcgacat ccccaccggc
    10621 gtgccgttgc tctacgagct ggatgggcaa ggccgggtgc tcaggaagga aacgctcggc
    10681 ggccccgacg accctcgacg ctgaggcacc cgcccccctg atctgcgcct tcttcgaggg
    10741 ccggctcagc gcgatcgcca tcccggcctt cctgtcgacg gcccgagccg ggccaaggag
    10801 cgcacccgcc cctactttgc cgtcgcccgg tcgctgcgcg cccggtgggc tccggagagc
    10861 tccgtcatcg cttgatgctg gctgaggaac acccggccgg tcaggtggtc gaggaagtcg
    10921 acgcgctgca gacggtccat gacggggccc ttgacctccg acaggtggaa ggtgacgccg
    10981 ctgtcggcga ggcggcggtt gatctcctcg aggctctcga gcgcactggc gtcgatggcg
    11041 ttgacggccg ggcacatgag gacgacgtgg cgcaactccg ggcgggcggc gaccatgtcg
    11101 tagaggcggt cctcgaggta gcgggcattg gcgaagtaga ggctctcgtc gacgcgcagc
    11161 gacaggacgg ccgggtcggt ctcgacctca tggcgaagca cattcctgaa atgctcgctg
    11221 cccggcacgc ggccgacgac cgccagatgc gggcggctgg tgcggtagag atggagcacg
    11281 agcgagacca gcacgcccgt ggtgacgccg atctccacgc cagcgccgag ggtcagcagg
    11341 atggtggcgg cgacggcggc gaaatcggcc ttggcatagg tccaggttcg cttcaggaca
    11401 cccagatcga ccagcgagag aacagccacg atgatcgtgg cggccagcgt cgcctggggc
    11461 aggaagaaga gcagcggcgt caggaacagt gtcgcgaacg cgatgccgac cgcggtgaag
    11521 gccccggccg ccggcgtctc ggcaccggcg tcgaagttga cgaccgaccg cgcgaagccg
    11581 ccggtcaccg gatagccggc tgagacggcg gaggcgaggt tggcggcacc gaggccgacc
    11641 agctcctggt ccggatcgat acgctggcgg cgcttggcgg cgagggtcga ggcgaccgag
    11701 accgattcga cgaagccgat gacgctgatc aggatggccg gcacgaggag ccggcccagg
    11761 agctcgaggt cgaggccggg cagcgcgaag ggcggcaggc cttgcgggac ggcgccgacg
    11821 atgcgcacgc cccgttcctc gagcgacaag gcggacgaga gggcgatggt cacggcgacg
    11881 gcgatgatcg gcccggcctt ggcgagggtg tcggcgaggc ggggccgcag gccgaggccc
    11941 acgagcagcg gcttcaggcc ccgcctgacc cagaagagga aggccatggc ggacaggccg
    12001 atggcgaggg tccagggatg ggttgccggc aggttccgga tcagggatcc ggcgatggcg
    12061 accaggttgc tgccgccggc cgggacaccg aggatgtgct tgagctggct cgcggcgatg
    12121 aggatgccgg aggcggtgat gaagccggcg atcaccggat ggctgaggaa gttggcgatg
    12181 aagcccaggc ggagcagccc gagaccgatt cgacgaagcc gatgacgctg atcaggatgg
    12241 ccggcacgag gagccggccc aggagctcga ggtcgaggcc gggcagcgcg aagggcggca
    12301 ggccttgcgg gacggcgccg acgatgcgca cgccccgttc ctcgagcgac aaggcggacg
    12361 agagggcgat ggtcacggcg acggcgatga tcggcccggc cttggcgagg gtgtcggcga
    12421 ggcggggccg caggccgagg cccacgagca gcggcttcag gccccgcctg acccagaaga
    12481 ggaaggccat ggcggacagg ccgatggcga gggtccaggg atgggttgcc ggcaggttcc
    12541 ggatcaggga tccggcgatg gcgaccaggt tgctgccgcc ggccgggaca ccgaggatgt
    12601 gcttgagctg gctcgcggcg atgaggatgc cggaggcggt gatgaagccg gcgatcaccg
    12661 gatggctgag gaagttggcg atgaagccca ggcggagcag cccgaggccg acgaggagga
    12721 ggccggagag cagggcgagc gccgtggccg ccgtggcgtg gtcggccgtg ccggcagcgg
    12781 cgatctcgcc gacggcggcg gcggtcatca gcgagaccac cgcgaccggc ccgacggcca
    12841 ggctgcggct ggtgccgaag acggcatagg cgaccagcgg caggatgctg gcatagagcc
    12901 cgacctcggg cggcaggccg gccagcatgg cataggccag cgactgcggg atcagcatga
    12961 tggtgacgat gagcgcggcc acgagatcgc cggtggccgc cccgcggcca taggtccggc
    13021 cccagtcgag gatggggaag tagcgggcga gcccgctcgg ccggtgattg ccgttcggcg
    13081 cggcgccggt ccgctgcagg tcagtcatct tcgctcacac caggtcggtc gggcgacgtg
    13141 cggtcgtcag ttctggaatt ccggcttcgc cagggtcgcc cgaccctcga gcatgccctg
    13201 ccggtggatc ggcggcagca tgcgctcctt ggggaaccag gcgaggcgcg aaggctcagt
    13261 gcccttggtc aaccagcacg ggaagctcgg caggcgcttg ccgccatagc cgaactcggc
    13321 cagcacgatc tcgccgtgct cgacggtcag cgggcccgag ccgtagccgt catggatcgc
    13381 ctgcgggccg tcggcctggc cggtggccgg ctcgacgatg gcgatgtcga gctcgggctg
    13441 ccgggcgccc tcgccgtccg gccggtcgac ggtcagcggg cgaaagccct gctgcctcgc
    13501 tgcgaccacg tcgccggccg tgagccggga agctaccgag agctgctcgg tgggctgcct
    13561 tgcctgcatc agtcacgcct cctctcccgt tggaaccgct cagagcgtgt tcaggggcac
    13621 cttgaggaac cgcttgccgg aggcatcggg ctcgggcagc cgaccggcct tcatgttgac
    13681 ctggagcgag gggatgatga gcttcggcat gtcgagcgtc ctgtcgcgcg cctcgcgttt
    13741 ggcgacgaac tcgtcctcgg tggtgccgtc ccggacgtgg atattgtgct ggcgctcctc
    13801 cccgaccgtg gtctcccagg cgatgtcgcg gccgttcggg ccgtagtcgt ggcacatgaa
    13861 gagccgcatc acgtccggca gctcgagcag ccggcggatc gagcggtaga gggtgcgcgc
    13921 gtcgccgccc ggaaagtcgg cccgggcggt gccgccgtcg ggcatgaaga gcgtgtcgcc
    13981 gacgaaggcg gcatcgccga cgatatgcgt catgcaggcc ggcgtatggc ccggcgtatg
    14041 aatggccacc gccggcatcg tgccgatggt gtagctgtcg ccgtccttga acaggcggtc
    14101 gaactgcgag ccgtcgcgcc tgaactcggt gccttcgttg aacaccttgc cgaaggtctc
    14161 ctggacgacg gtgatgttct cgccgatgcc gagccgaccg cccagctcgc gctggatgta
    14221 cggggcggcg gacaggtggt cggcatggac atgggtctcg atcagccact ccacctgcag
    14281 atcctcgcgg cggacgaagg cgatgatggc atcggcatga tcgaaggtga tacggcccgc
    14341 ggcataatcg atgtccatca ccgaatcgac aaccgcgcag gccgtcgatg cgggatccct
    14401 gacgacatag ctgatggtgt tggtcgccgg atcgaagaag gcggtcacct ccggccgcac
    14461 gtcgagatcg atcttcggaa cgaagctgtt ggtgacgctg ctcatgcgtg tcctcctcga
    14521 ccgtcgggcc ttgaagcagt tttgagtgat gccattcaca tctaattaat cgaattcatc
    14581 aatcaagatg gtggcctgcc ccggcacgag gaacgggccg tcgcatcgcc cgggcttgca
    14641 accggcgcgc ggcatgggca aatagtaggc agcttggccg tggcccgggg ggacgtggcg
    14701 gcgccatgct tcacaccggc ttgggcggtc gagcgagcca accgggttgc aacgaagtcg
    14761 aagacccgtt cgacaacgtg tgatcacgac gagattcgaa cggagagaca gggatgccgt
    14821 cttcatcgcg ctgcgccgag gcgcctcgcc cgagccgtcc ggccaaggag gccaaagcct
    14881 ggatggcgcg gctgagcgcc taccgcgtgc cgagcacgtg gcgcagcctg ttcgagctcg
    14941 ccgtgacctt cggcccgttc gtggtcgcct ggacgctgct cgccatcgcg gtgcatttcg
    15001 accagctctg gctctacgcg ctcctgctcc tgccggccgg tggcctgctc gtccgcctct
    15061 tcatgatcca gcacgattgc ggccacggct ccttcctgcg gcggcgctgg agcaacgact
    15121 ggatcgggcg ggccatcggc atcgtgacgc tcacccccta cgaccactgg cggcaggccc
    15181 acgccgtcca ccatgcgacc accggcaatc tcgaccagcg cggcgtcggc gacatcgaca
    15241 cgctcaccgt gcgcgaatac cgtgccctgc cctggcggcg gcggctgctc taccgcctct
    15301 accgcaatcc cctggtgatg ttcggcatcg gccccttcta catgttcgtc ctgcagaacc
    15361 gcctcccgct cggcgccatg cgcaagggct ggatgccctg ggtgagcacc atggcgacca
    15421 atgcggctat cgtcgcggcc gcggctcttc tgatctgggc catgggcctc ggaccgttcc
    15481 tgctcgtgca cctgccggtc gtcgccctcg gcgccaccgc cggcgtgtgg ctcttctacg
    15541 tccagcacca gtacgagggc acgctctggg acaagggcga cgactggaac gcgcacgagg
    15601 cggcgctgca cggcagctcg cactacgacc tgcccgccat ccttcgctgg ttcaccggca
    15661 atatcggcat ccatcacgtg caccacctct cgagccggat cccgttctac cggctgcgcg
    15721 aggtgctgcg cgatcacccc gagctgcgcg aggtcgggcg gctgacgctc tggcagagct
    15781 ttcgctgcgt ccgcctcgcg ctctgggacg aggagaagcg gcgcatggtg accttccgcg
    15841 aggccgccgc cagcgcctga ggcgccttca ggccgggatc tcgtccgtcc agacccgaaa
    15901 cccggcgagg aagttcggcc cgaaggtgtg ggtcaggggc acgtcggcgg cgtcgcggcc
    15961 ctgggcgatc ttgatgcggc cgtacatcgg ccggttgttg cgcggatcga agacccacca
    16021 gctgccgccg agatagacct ccatccaggc ggcgaagtcc atcgacgcgt agggcggcgg
    16081 ctggccgata tcgctgacat agccggtgca gtagcgggcc ggcaggttca tgcagcggca
    16141 gaaggcgatg gcgaggtggg tgaagtcgcg gcagactccc cgcccctcgg cataggcctc
    16201 ggctgcggtc cgggtcgacc gagagtgctc gtagccgaat gtgatgtggc ggttgacgaa
    16261 gtcgcagatc gcctggaccc gggcccagcc tgtcggcccc tgcccgaaca ggcgccaggc
    16321 ctcccgggac aggcagtcgg tctcgcaata gcggctgccg aggaggaact gcaggctgtc
    16381 gggcggcaga tgctcgaccg gatgctgcat ggcatccagg gccaccggat cgctctcccc
    16441 ggaatcgcgg atgacgctgt cggtgccgag cacgaagcgg ccggacggcg ccacgagccg
    16501 gcagcaccag ttgccgaagc cgtcgcgata gctctcgatc ggcaccgccg gcgtggtcat
    16561 cagccagtcc ggccgttcca gccgggcgac gcatgaatgg tgcacgttga gcagcacgat
    16621 catcggcgtg ggattcggca cctcgtaggt gagctcgcag ccgaggtgga ttcgcatggc
    16681 gttctcccta gaatggcgcg accgctacgg cagcatcgca ggtttgacgg tatcgtcgag
    16741 gccggcgcgg cagttcctcc agagcgggct catcgatgtc gcacgatcac gccggtcatt
    16801 cccatgccgc cccggcgacc gaggggcggc tggcgctcgc cgtcctcgtc aatgtcgggc
    16861 tgacggtcgt ccaggtggtc ggcggcattc tctcgggcag cctggcgctg gtcgccgatg
    16921 ccctgcacaa tttctcggac gccgcctcgc tgggcatcgc ctggggtgcc cggcgcatcg
    16981 cccggcgacc ggccgacgcc tcgatgacct tcggctacgc cagggccgag atcgtcgccg
    17041 cgctgatcaa cttcacgacg ctcattatcc tcggcctcta cctcgcctac gaggcggtgc
    17101 tcaggctcct cgacccacag ccgatcctgg gctggacggt cgtggtgatc gcggcgatcg
    17161 ccctcgtcat cgacctcgtc acggccttcc tgacctatgc cggctcgaag gacagcatga
    17221 acatccgtgc cgccttcctg cacaacgtgg cggacgcgct gggctccgtc gcggtcatct
    17281 tcgccggcac gctggtgctg ctctacgact ggacgatcgt cgatcccctg gtgaccctgg
    17341 cgatcgccgc ctacgtgctc tggcatggcg gcacggagat cggcggggcc atccgcatcc
    17401 tcatgaacgc ggcacccgac ggggtcgaca cacaggaggt ggcgggtgcc atgaccgcaa
    17461 ctgccggggt cgccgatgtc caccatgtcc atctctggcg gctcgacgag cgccgcacct
    17521 cgctcgaggc ccatgtcgcc ctggacgccg acgcggcgcg gcacccggcc gccgtcaagg
    17581 cgcggctgcg aacgatgctc accgagcgct tcggcgtcgg ccacgtcacc atcgagaccg
    17641 agctgccggg cgaggcctgc gccgcccccg gcggtgtcca gccgcagggc gggcgaccgg
    17701 ctgccggggt ggcagccgac tagaaaagat cccaggcgag atagcccaca taggcggcat
    17761 agagggcgag cagcaccgcc ccctcgcggc gcgagacgcg ccagccgctg atcgcgaaga
    17821 agagcagcag cacggtggcg gccagcatga cccagatgtc gaagtccgcg atctcgcgcg
    17881 gcacgtcgag cggctgcacg gtcgcggtga tgccgaggat tcccagggtg ttgtagatgt
    17941 tgctgccgac gatgttgccg aacgccacat cggcctcttt cttcagggcc gcgatgacgg
    18001 ccgtaaccag ctccggcagc gaggtgccga ccgccacgac ggtgaggccg atgagcgtct
    18061 cggagacgcc catggcggtg gcgagctcga tggcgcccga gaccagcagc cgggcgccga
    18121 cgatcgtgag cgcaatgccg gccagcgcga tgagcaacgc cagccagagc ggcatcggct
    18181 gctcggtgag ctgcaacgcc gcctcggcct cgtgcatctg tgccgcctcg tccgggctgt
    18241 cgctctccct gacataggtg aaggcgacat aggccaacaa cgccgcgacg aaggcgaggc
    18301 cggtccagcg gtcgagcgca cccaccagga ccaccgcacc gagggctgcc gcgctgagca
    18361 cgaggacggt gccgtcgcga tagaaggccc cgggatgaca ggccatcggc agcaacagcg
    18421 caccgacacc caggatcagg aggatgttgg cgatgttgct gccgacgata ttgccgacgg
    18481 cgatgccggg cgacccggcc agggccgcct gcacgctggt caccagctcg ggcgtcgacg
    18541 tgccgaagcc gaccagcacg atgccgatga agagaggcga gaggccgagc cgcgtcgccg
    18601 ttgcgaccgc accgcgcacc agcgattcgc cgccgacgac caggagtacg aaaccgagta
    18661 tgagcgatgc cagcatcggg gccagtccag gtgcgatcga agggttcggc gggtcggagc
    18721 agaacgacgg cgtgccgcgc atgaggtgga gcacaccgga cgatgtggca agccgaaaac
    18781 ctggcgctgg tggcgctgac cttcctcgtc gccgggttcg tgaaggggat cgtcggcctc
    18841 ggcctgccga ccatcgtgct cggcgtcctc accaccacct atggcatcac cccggcgatc
    18901 gcgatcatgc tggtgccgtc cctggtcacc aacgccattc aggggctcgg cggccagacg
    18961 tcgctcgccg tcctgcgccg cctctggccc cttctcctgg ccgctgccgt gggcatcgtc
    19021 gtcggcgccg gcgtgctggc ccgcagctcg ggcagcggcc ctgccgtcgc cctcggcctg
    19081 ctcatcatgc tgcacggctg catcagcctg ctcgggcccc ggctgccgca tccgggtccc
    19141 cacgagcgct ggctctcgcc tctggccggg ctcgccaacg gcctgatcac cggcttcacc
    19201 ggctcggcca tcctgcccgg catgctctat atccaggcgc tcggcttcga caaggagcgg
    19261 ctggtccagg ccatgggcct gctcttcggc atctcgaccc tggtcctgat ggtcgccttc
    19321 gcccgctacg agctcctgac accggacctg gcgctgctct ccgccctcgc cctgctcccg
    19381 gcgctgctcg gcctcaggct cggtgccagg ctccggcgac ggatcgccga ggagcgcttt
    19441 cgccggctgc tctatgtcgt gctgattgcc ctggggggag cgatcgtctg gcgcaatatc
    19501 ggctgaccgt tcagcgccgc cggcggttca gggcgctcac cggcccgagc ggatgatgcg
    19561 cgtgcggata ggggtggtga tgatggtcgt cgtgcccgtg atgatgatcg ggtccgtggt
    19621 gatgatcatg ctcgtgcccg ggcgcatgct cgtgcccgtg atcatgcgcg tgggcatcga
    19681 ccagccggca ggcgccggtg cagaaatcgt cgcagagcgg gcagtcggcc accgatgagc
    19741 cgggggccga agcccccttg ccctcgacat gatggtggtg gctcgcctgc ggcagcccga
    19801 cctccgcctc gaagcccagg acctgggccc ggtacttgca gagcgagcag ttcatcaggt
    19861 tcgccccctc caggatctcg cggacccggg cggccatggt ctcgaccacg agtggatggt
    19921 cgccgagata gccggcctcg acgaaccgga tcgacggatg gcgcgccgcg acctcgcggg
    19981 tgaagccgaa gatccggtcg accaggatgc cggtgaagag aaaatagggg aagaccacga
    20041 cccgcttgta gccgagcttc gccacatgct cgaggcaggg ctcgacaagc gggaaggtga
    20101 cgccggaata gccggtctcg caccagccga agcccatgcc ttcccagagc atccgggcga
    20161 tcttggcgac gttggagttg gcgtccgggt ccgaggcgcc acggccgatg acgacgaggc
    20221 agaccttgtg ccgcggcatg gcgccatgct cggcatccgc gcgggccagg gccgcctcga
    20281 tgcggtcggc cgcggcccgg atcatccgcg catcgacggc gagctcgcgg ccgtagtcga
    20341 tccgcaggcc gtgcgccttc tggtaggtgt tgagcacgga agggatatcg ttcttggcgt
    20401 ggccggcggc gaacagcatg cccggcaccg cgagcacgcg ggtgcagcca gcctcgcgca
    20461 gccggtcgag cccgtcccgg atgaccgggt tggcgaactc gagatagccg tagctgaccg
    20521 gccagtcggg aaaggcgccc tccagctttt gcgccaccgc ggcgaactcg gcgaccgccg
    20581 ccgtcgagcg cgagccgtgc ccacacacca tcacgccgat ccgttcgccc atcgagccac
    20641 gatccccctg ccggatttgc caccccggac ataaccgttt gccagccgac cgcaaggctc
    20701 tgacaatgcc tcagtcggcc cggcggcggc cacggcatcg gttgcccgcc accggcggca
    20761 gcatctggca cgtctcggcg gagcggcagg ccgcatggca ctcaagacac agatcgtcgt
    20821 ggtcggcggc ggtgccggcg gcctggagct ggtggcgcgg ctcggtgcca ggcacggccg
    20881 ggacggccgc ttcgacatca ttctggtcga agagaaacgc acccatatct ggaaaccctt
    20941 gctgcacgag gtcgcgaccg gcgcgctcga cgccaatctc gacgaggtcg gctatcgcgc
    21001 ccatgcctat cgctggggct accgctactt catgggccgg ctcgaagcca tcgaccgcgc
    21061 cgccggccag atcgtgatcg ccccgatcct cgacgaggac ggcaaggagg tcatgggccg
    21121 ccatctcatc cgctacgact tcctggtcct ggccctgggt tccgtcacca acgacttcgg
    21181 cacgcccggc gtcgccgagc actgcatctt cctcgaccag cgcacggccg ccgaccgctt
    21241 ccggcagaag ctcctcgacc actgcctgcg cgtctcccgg accctgtcga tggacccccg
    21301 ggccgacgcc catgtcgacg ttgccatcgt cggcggcggc gccaccgggg tcgagctcgc
    21361 ggccgagctc tacaatgccg cggctgccct tcggcactac ggcctcgagg tcttcgacga
    21421 gagccggctg cgcgtcgctt tgctcgaggc cggcccgcgc ctcctgcccg ccctccccga
    21481 gaagctcgcc caggcggccc gggacgagct cgagagcctg ggtgtcaggg tgctcgtcga
    21541 gacccgggtc acggaggtca ccaacgccgc catgcgcacc cataccggcg aggtgatcag
    21601 cgccgatctc cgcgtctggg ccgccggcat caagggcccg gatctgcttt cggacatcgg
    21661 cgggctcgag accaaccgca tttgccagct catcgtccgg ccgaccctgc agaccacgcg
    21721 cgacgcccgc atcttcgcgt tcggcgactg ctgcagctgc acgcccgaag gtgccgagcg
    21781 accgattccg ccacgcgccc aggcggccca ccagatggcc cggaccgtct ttcgcaacct
    21841 gcagcacctg atgcagaaca gacggctcga gcacttcgtc taccgggatc acggctcgct
    21901 cgtctcgctc agccgcttct cgaccgtggg cagcctgatg ggcaatctca tcggcgggcg
    21961 aatggcgatc gaggggcggc tggcgcgctt cgtctacgcc tcgctctact ggaagcacct
    22021 cctgaccatc cacggctggc tgcgcggcat cgcccatatc gtcgtcggcc aggtcaacca
    22081 cgtcatccgc ccgcgcctca agctgcactg atggaccgga cgtccgaccc gcgccgccat
    22141 gccccggcca ccctgcgcaa ccgggccccg atcctggcgg tgctgcagcg tgtgctgccc
    22201 gccagcggcc tgctcctcga ggtcgcgagc ggcagcggcg agcatgccgc ctggatggcg
    22261 ccacagctcc ccccgggcct cgcctggcag ccgagcgaag ccgatgccct ggccctgccc
    22321 ggcatcgacg cccatgcccg ggacgccggt gccacgaccg tccgcccggc gatccgcctc
    22381 gatgcggccg ctgcggattg gccaatcgcc gcggccgacg cggtcttctg ctgcaacctc
    22441 gcccacatcg ccccctgggc cgcgaccgaa gggctgctcg ccggtgccgc acggttgctg
    22501 ccgcccggcg ggccactcgt cctctacggc cccttccggc gccacggccg gcacacggct
    22561 gcgagcaacg ccgccttcga ggccagcctc caggcccggg accccgcctg gcgcatccgc
    22621 tgcgtcgaca atgagctcgt cccggccgcc gcccgccacg gcctcgtgct cgacgaggtg
    22681 gccgagctgc cggcgaacaa cctcgtcctc gtctggcggc gggagtcgct cagcgctgcg
    22741 tgagcagccc gtactccgcc ggcatccggt gcgccttgaa gtcgaagatg ttggccttga
    22801 tcgactggca gcggtcgagg tcgcaggccg ccacgcagat ctcgtcctcc agggtcgagc
    22861 agagcgccac cacctcgccc gtgggggcga cgatgcagct ctcgccgatc agcatgcagc
    22921 cttcctcgac gccgcatttg gcgacaccga ccacccaggt gccgttctgg taggcgccgg
    22981 cctgcagcac gaggcggttg tggaactgct gcagatggtc ctgctcgggc accaggggat
    23041 aatgctgcgg cgtgttgtag ccgatcgcca ccagctcgac gccctgcagg cccagcatgc
    23101 ggaaggcctc gggccagcgg cggtcgttgc agatcagcat gccgagcctg ccgcccatgg
    23161 tgtcgaacac cgggaagccc aggtcgcccg gctcgaaata gcgcttctcg agatgctgga
    23221 acggtcgcca ggcctcgacc tcgcggtggc cgggcaggtg gatcttgcgg tacttgccgc
    23281 tgatccggcc atccggctcg accaggatcg ccgtgttgaa atgccgcgcc cggccttcgg
    23341 cgtcggtcac cagctcggca tagcccagcg cgaagccgag accgagctcg ccggccgcgg
    23401 cgaagagcgg tgccgtctcc ggccccggca tctcccgctc gaagaaggca tcgacctcat
    23461 ccggctcggc cagcccccag cgcggaaaga aggtggtgag cgccagctcc ggaaagacga
    23521 cgaagccggc accacgcgcc ttcgcctgcc ggaggaggtc gatcagccgg ccgacgacac
    23581 gggcccgcga atcgccccgg gcgatcgggc cgagctgggc tgcggcaacg gtgagcgtgc
    23641 ggctcatggg gcgatctcct ccgctggacg accgaccggc tcggcgccaa ccagcctggc
    23701 atagagctcc gggtcggtgg caccctcggt gccgaatacg agcaggcggc tggccgggcc
    23761 gagaccgagt agctcggcga tctcgggata ttccagggcg gcgagtgcgc cggcgaggcc
    23821 gcaagtggcg ctctcgcccg cgaccaccgg cgggtcgttc cgggcgaggt cgcgcatcac
    23881 cgtcaccgcc gccgcgtcgt cgatcacgag gacatcgtgg acggcgaggc gcagcacctc
    23941 ccaggcgacg agcgagacct cgccgcaggc gagcccggcc atcagcgtgt cgaggtcgcc
    24001 ggcgaccgcc cggcgctcgc cggcctcgat gctggcggcg atgcaggccg cccgttccgg
    24061 ctcgaccacg atgaagcgcg gccggtccgg ccccaggatc tgccaggacc gcgccgcgac
    24121 cgcggcggcc acgccgccga ccccgccctg caccaggacg tgcgtcggca tcggcgcccc
    24181 ggccagttgc gcaaatgctt cgtccgccat cagcagatag ccgcgcatga cgtccgccgg
    24241 cacctcggtg tagccgggat aggaggtgtc cgagacgacg tgccagccgt gccgggccgc
    24301 gtcctcggcc gcgcggcgga ccgaatcgtc atagttgccg ggggtgcgga cgacctcggc
    24361 gccgaacgcc tcgatcgccc gcttgcggcc ctggctcacc gtggcatgga tgtagatccg
    24421 gcagcgcgcg ccgaagcgcc gggcgcccca ggcgaccgag cggccatgat tgccgtccgt
    24481 ggcgcacgtg acggtgacgc tgcgcgcgaa tcgggcgagc tcggccgcgt ccctcgccgg
    24541 tcgccgctcg cgctcgacga agagccgctc cagaagacgc ctcaccgcat aggccccacc
    24601 cagcgccttg aagctggcga ggccgaaccg tgtagcctcg tccttgtagt ggatggcggc
    24661 gaccccggcg gcctctgcca tggctcgcag atcatgaagc ggcgtcggcg catagcctgg
    24721 ccagctcgtg atctcggcgc gcaccgcctc caggtccgcg gcggcgagaa tgggctcgag
    24781 cgccggcgga taggggaaat gccgcagcac ggcaggattg gcgatgtggg cgggccgggc
    24841 aaagcgggac aagcggcagg ctccggaact ggcatggtca cccgtgatcc atagcccggc
    24901 agcaacgggc tgaccatctc cggccgcatg agcaccggcc ggagggtcgc acctctcaac
    24961 tgttgcgttt gacgcactgc ggcatcctgg atggcgccag gggccattcg gggtttgcaa
    25021 cttgccatcc gaagtggcat ctctgctgcc ttcgccgggc cggctgcccg ggcgcgctcg
    25081 ttcgtggttc ggctcctcga atcaaccggc gtgttccaca cctacctgat gcaccgagcc
    25141 ccatgaccga gacccgacag gatatcgtgc agtacgcggc gcgcttcgat cccgccatgc
    25201 cacggcggtc cgcgacgctg tcgagccatc tgcgcgcgct ggaggccgaa tcgatcttca
    25261 tcatgcggga ggtcgcggcc gagatggcca accccgtcct gctctattcg atcggcaagg
    25321 acagctcggt gatgctgcac ctggcgatga aggcgttctg gccggcgaag ccgcccttcc
    25381 cgctcatgca catcgccacg ggctgggact tccgggacat gatcgtgcac cgcgatcagc
    25441 tcacacgtgc cctgggcctc cggctgctgg tccagtcgaa cgagaccgcg acggccgagg
    25501 gcagcacccc cttcaacacc gacagccgcg aatgggcacg gatcatgctg atcgatgcgc
    25561 ttaaggccgg catggaccgg catggcttcg atgcggcctt cggcggcggg cggcgcgacg
    25621 aggagaagag ccgggccaag gagcgggtgt tctcggtgcg ctcggccggc cacggctggg
    25681 acccgcgcaa ccagcgcccc gagctttggc acctctacaa tacccgtgtc gccaggggcg
    25741 agacgatccg cgtcttcccc ctctcgaact ggaccgagtt cgacatctgg gagtatatcc
    25801 gcctcgagca gatcccggtc gtgccgctct acttcgcgaa gcgccggccc atggtcgatc
    25861 gcggcggcac gctgatcatg gtcgacgacg agcgcatgca gttccagccc ggcgagacgg
    25921 cgtccgagcg cctcgtgcgc tttcgcacgc tcggcgactg gccgctcagc ggggcgatcg
    25981 aatccgacgc cgacacggtc gagcaggtga tcgccgagat gctctccgcc cggacctcgg
    26041 agcgccaggg ccggctggtc gacagcgacg aggcggcgtc catggagaag aagaagcgcg
    26101 agggctattt ctgatgacga gacaggccga agcggtcgcc atcgccaccc gggccgagga
    26161 agcggccaag ggcttgctgc gctttttgac ctgcgggtcg gtggacgacg gcaagtcgac
    26221 cctgatcggc cggctgctct acgattcgaa gctgatcatg gaggatcagc tccggtcgct
    26281 cgccgacgat tcgaagaagc acggcacggt cggcgaggac atcgacctgg cgctgctcgt
    26341 cgacgggctc gaggccgagc gccagcaggg catcaccatc gatgtcgcct accggttctt
    26401 ctcgacggca cggcgctcgt tcatcgtcgc cgacacgccg ggccacgagc agtactcgcg
    26461 caacatggcg acgggtgcct cgacctgcga cctcgcgatc atcctgatcg atgcccgaaa
    26521 gggtgtcctg acccagacgc tgcgccacag ctatctggtg cacctgctcg gcatcaagca
    26581 cgtcgtcctg gccgtgaaca agatggacct cgtcggcttc gccgaggggc gcttcgccga
    26641 gatcgtcgcc gactatcgag cggccaccag ccagctcgcc ttccgggacg tcctgccgat
    26701 cccgctctcc gcgcgcttcg gcgacaacgt caccatgccg agcaaccgca tgccctggta
    26761 tcgcgggccg atgctgctcg agcacctcga ggcggccgac gtcacggccg aagccggacg
    26821 ctccgccttc gccctgccgg tgcaatgggt caaccggccg caccaggatt ttcgcggctt
    26881 cgccgggacc atcgtcagcg gccggatcgc cgcgggtgat cccgtcgtcg tggcgaagtc
    26941 cgggcgggcg acgcgggtca gccgcatcgt caccgccgac ggcgaccggg ccgaggcctc
    27001 gacgggccag gcggtgacgc tggtgctcga cgaggagatc gacatcgccc ggggtgacgt
    27061 gctggtcgag ccccgggcac gccccgaggt ggccgaccag ttcgccgccc acctcttctg
    27121 gatgcacgag gagcccctgc tgccgggccg gcgctatctc ctgaagctcg gcgccgagct
    27181 cgttccggcg acgatcacct cgatccgcca caagatcgac gtcaacacgc aggaggagcg
    27241 cccgggccgc acgctcgagc tcaacgagat cgccttctgc aacctgcata ccacccagcc
    27301 gatcgtcttc accgcctaca cagagaaccg cgatctcggc ggcttcatcc tgatcgaccg
    27361 gaccaccaac cagacggtgg gcgccggcat ggtcgagttc ggcctgcgcc gggccagcaa
    27421 cattcaccac cagcatctcg acatcgacaa ggcgcgccgc gccgcgcaga aggggcagaa
    27481 gccctgcctc gtctggttca ccggcctctc cggcgccggc aagtcgacca tcgccaacat
    27541 cctcgagcgc aagctcgtgg cgcagggtgc gcacacctac ctgctcgacg gcgacaacgt
    27601 ccgccacggg ctgaacaagg atctgggctt caccgacgcc gaccgggtcg agaacatccg
    27661 ccgcgtcggc gaggtggcca agctgatggt cgatgccggg ctcatcgtcc tggcttcctt
    27721 catctcgccc ttccgggacg agcgccgcat ggtgcgcgac ctcctcggcg agggcgagtt
    27781 catcgaggtc ttcgtcgaca caccgctcgc catcgccgag acgcgcgaca ccaagggcct
    27841 ctacaagaag gcgcgggccg gccagatcaa gcacttcacc ggcatcgatt cgccctacga
    27901 gccgcccgag caggccgagg tcaccgtcgc caccggcgag ctctccgccg aggctgccgc
    27961 cgaccagatc ctggcctacc tgacgtcaca cctctgaggc ccgtcatccc cgggccggcc
    28021 gtgcccgggt cggtcggctc cgtcgc
//
LOCUS       DAOTFI010000023        27431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_1999_length_27431_cov_7.588727, whole genome shotgun sequence.
ACCESSION   DAOTFI010000023 DAOTFI010000000
VERSION     DAOTFI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 27431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27431
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_27431_cov_7.588727"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="P5558_03670"
     CDS             complement(<1..189)
                     /locus_tag="P5558_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23441.1"
                     /translation="MQRGLGFVLAVAVLARGGTARAAEAGVVAWLRGTATATTDGGGT
                     RILHVGAVVHEGDELRTLA"
     gene            complement(261..2075)
                     /locus_tag="P5558_03675"
     CDS             complement(261..2075)
                     /locus_tag="P5558_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23442.1"
                     /translation="MAKTTIASASRLGLGSLAGALLAGSAFAQPATPDISELLDPARV
                     AASACGGLRYARNELFKPGVQLAALASPAAAVEASPGSPLPILDGLGPDRLQATTRSD
                     EARALAAQGYAWLMGFNHRLAAAYFRAAQAADPGCAICYWGEAYALGPNINDAMHADA
                     VRPAFQAMAMATLLIEGASPREQALIRALKTRYSMAADADRAALDQAYADAMLAAADA
                     HPDDVELLVLAAEAVMNSQPWDYWEADGVTPKGRAGRLVALLETALAADPDHPHAIHL
                     YIHTVEASSDPARAEPHADRLAALMPTAGHVVHMPAHIYYRVGRYQDSLATNIAAAEA
                     DEALFAEWQAAGEEGGLYRFGYYPHNVHFVLTSATMAGDAISAMAAAHKLAAVMSDEV
                     AAEVAWVQAIRTAPFTATAQLGELDAVMALPDPGDAMPFVKGLWHYARGVALARAGDT
                     DAAAGEAAAIEALALDPQLQLLVEQYVPAPDLLALAAHVVRARIAVAAGDFATAESRL
                     LEAVALQATVPYMEPPYWHYPVVQTLAAVELMAGEPEAASRHFQDALMATPNNGWSLF
                     GLMEAQRAMGDEAGAKVTADLLDRAWVGPRELLQVERL"
     gene            2231..2512
                     /locus_tag="P5558_03680"
     CDS             2231..2512
                     /locus_tag="P5558_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23443.1"
                     /translation="MSEATTTTDHATIRRWVEARDGRPARVKATAGGEPGSGSGGILR
                     IDFREPDEALEELSWEAFFEIFDQNRLAFLHQDKTADGSTSRFFKFVER"
     gene            2543..3643
                     /locus_tag="P5558_03685"
     CDS             2543..3643
                     /locus_tag="P5558_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY23444.1"
                     /translation="MVSRVLVTGGAGFIGRRVVAALLARGHEVRVLDALIEQVHGGGR
                     PALADVELRVGDVRDGEVVGRALAGMDAVVHLAAEVGVGQSMYEIERYVGVNELGTAT
                     LFQKLVERPVERVVTASSMSVYGEGLYRERGGRIRADVERPRPAGGGWDPLDGAGLPL
                     VPVPTPEWKAPSLASVYALNKYAQERLTHVVAKAYGMAGVALRLFNVYGSGQALSNPY
                     TGVLAIFAARLLNGQAPMVFEDGEQRRDFVHVDDVAEAFALALETPAAAGRTFNIGSG
                     VDVSIAEVASRLAAAMGRPDIRPLITGKARAGDIRHCFADIGAAREVLGYAPRTGFDE
                     GLAEVVAWVARQRAVDRVEEARRELEERGLVA"
     gene            3640..5640
                     /locus_tag="P5558_03690"
     CDS             3640..5640
                     /locus_tag="P5558_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY23445.1"
                     /translation="MSAGLGVVEWFRPGDHSRVEASLERLRRLGCSRLRTHLSWADYH
                     AEGGRDWYDWLLPRLAGEVELLPCLHYTPPSLSETGAVAGPPRDLKAFADFLDHVVER
                     HGRHFAAVELWNEPNNLLDWDWRVDTDWSKFATMVGAAAHWGRRLGKQVVLGGPCPAD
                     MNWLELMGRRGVLGEIDALGLHGFPGTWDSEAAGWPGWNSLAEEALAVLDRHNPAVEL
                     WVTEAGYATWRHDEALQARAFLELHRAPFARVYWYMLQDLADEVAVQEGPRFDERHYH
                     MGLFDKAGRPKLLARLLAEGGPAKVAEVIERAPPAPAIVGTRPVLVTGGAGFIGSNLA
                     DRLAAEGEHVLVLDSLARPGVERNLAWLEQRHRRHVSSALVDLRDRDGVAAAAADAAA
                     VFHMAAQTAVTTSLVDPFGDFDVNLAGTINLLEAVRRRPVPVIMASTNKVYGDLGNVE
                     LIREGERWLPRDPRLRCHGIDETRALSFHTPYGCSKGAADQYMLDYARSFGVPTAVLR
                     MSCICGPRQLGTEDQGWVAHFLIRALEGRPITIFGDGRQVRDVLFVDDAVAAYLGLWR
                     AIDRIAGRAFNLGGGAANAVSLRQVLRAIGELAGAEVEVGYGDWRPGDQRWYVSDTRS
                     LSRAIDLPRPLGWRSGLERLARWLRTDGRDAEVLASPVAEVV"
     gene            5640..6935
                     /locus_tag="P5558_03695"
     CDS             5640..6935
                     /locus_tag="P5558_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04295 family B12-binding domain-containing
                     radical SAM protein"
                     /protein_id="HRY23446.1"
                     /translation="MRVALVNPRWDFTNSIYFGCPESHLPLELGYAAQRLEEAGHEAL
                     LLDGQLDGLENEALADRVADAGAGLTVVTTAPTYLFWRCAQPELRTPRAFLDALGGRG
                     GRTVAVGPHGSVTPAATLEKLGVDLVVRGECEEVIARLADAGGEPFGIAGTAWRDGDD
                     LEREGGPVAATFVDQKALFWPDAWIARHAHHHHRFDARPDVPGAEVEASRGCPYSCSF
                     CAKLDYRDKYRRRELGPLMEEIDRLIAQGVGYIYFVDEIFLPQKPLLEALAARDIVFG
                     VQTRIDLWSPPMLQLLGEAGCVSIEAGVESLTPEGRDALDKKCRMATDELAERLITAR
                     RFVPFVQANLIATAGDDPALVALWRERLRGAGVWANDPVPLYPYPSSPDYHKRWGAPD
                     DAAWERAHEHYLAQFERFSDIQDQRPLPLPELEAACRGA"
     gene            6920..8044
                     /locus_tag="P5558_03700"
     CDS             6920..8044
                     /locus_tag="P5558_03700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY23447.1"
                     /translation="MPGGVTEKRHRLLLTTDAVGGAWTWALDLAAAAIARGFRVRLLV
                     LGPPPSPDRRRQAAAVAGLELVETGLPLEWTAATPEEVIEASQQLASQVERQGPDLAH
                     LSNPALAAAGPWPVPLVATLHSCVGTWWAAMHPGRAMPDDLRWRQALVGRGLANADIV
                     TVPTLGFAQAASELYPDIVFEVVQNGRRADGEAPMGRPRARVLAAGRLWDEAKNMATL
                     DAAAARLAVPVEAAGPVVGPNGARIELPHLRLLGELGAGAMRQKLARTAIFVSPARFE
                     PFGLGVLEAAQGGAALLLSDIAVHRELWDGAAQFFEAEDAVSLAGAIAALMDDPARVE
                     RLAAAALSRAQAFGVDRMAAGMIALYERLLRHPLALSGAA"
     gene            8041..9150
                     /locus_tag="P5558_03705"
     CDS             8041..9150
                     /locus_tag="P5558_03705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY23448.1"
                     /translation="MNIAYLTHSLASCWNHGNAHFLRGLLRELGRRGHRCIAWEPEGA
                     WSLDHLVRDHGPEALRAFARTFPDLRRATYEDAQAAERIVDGADLVIVHEWTGPAMVA
                     ALGRLRARGGRFTLLFHDTHHRAVSDPGAVAALDLESFDGVLAFGESLAEVYRRRGWG
                     QRAFVWHEAADTTLFRPPVVAGPREGLVWIGNWGDGERTAELDSFLFRPAASQGLALD
                     VHGVRYPPDALERLAAHGFRYRGWVANAEVPGRFARHLATVHVPRRFYVEHLPGIPTI
                     RVFEALACGIPLLSAFWPDTEALFRPGTDHLVARSGAEMAAHMRAIRNDPELRAGLAA
                     AGLATIEARHTCAHRADELLAIVERLRGAATRVEA"
     gene            9150..10241
                     /locus_tag="P5558_03710"
     CDS             9150..10241
                     /locus_tag="P5558_03710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY23449.1"
                     /translation="MRIAFYGSSLVSAYWNGAATYYRGLLRHLAALGHEITFLEPDAF
                     GRQENRDIEPPDWARVVVWEATPEGLEVALGHAALADLVVKASGVGVFDDALLAGVLE
                     RSRPEAVRVFWDVDAPATLAAMRETPDHPLRRALPSLDAVFTYGGGDPVVRAYRGFGA
                     RVCEPVYNGLDTTTHFPVPADARFRADLAFLGNRLPDREARVHAFFLEAARRLPGHRF
                     LLGGSGWDDVDLPANVARLGHVPTRDHNAFNATPLAVLNIARDSMAQVGFSPATRVFE
                     AAGAGACLITDQWEGIEMFLEPGTEVLVARDGAEVAAHLAALDRKRAAAIGRRALARV
                     RRAHSYERRAAEVDLHLRRLVDDRRRVDA"
     gene            10238..11299
                     /locus_tag="P5558_03715"
     CDS             10238..11299
                     /locus_tag="P5558_03715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY23450.1"
                     /translation="MRIVILGLSLSSSWGNGHATTYRALVEALHARGHEVAFLERDVP
                     WYAENRDLDRSPHCDLHFYQTLDDLAALRPTITAADAVVLGSYVPEGVRVAEHLLAVA
                     EGPVAFYDIDTPVTLAKLEAGDHEYLTPALIPRFDLYLSFSGGAALEALVGRWGARLA
                     RPLYCGVDAERYRPLALGKRWDMGYLGTYSADRQPALERLLLEPARRLPERRFVVAGP
                     QYPRGIDWPANVERIEHVPPTGHAAFYGALRFALNVTRAAMIEAGHSPSVRLFEAAAC
                     GVPILTDRWTGIEDFFAPERELRIVDDTAMVVETLGGMAAERARAMGAAGRERVLRDH
                     TASRRAEELERSLAVAAGR"
     gene            11305..12072
                     /locus_tag="P5558_03720"
     CDS             11305..12072
                     /locus_tag="P5558_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HRY23451.1"
                     /translation="MADLADAIARLAPWFHNLHLPGGVETAPDHFLGDFPRFKWREIA
                     PHLPADLAGARVLDIGCNAGFYSFALAARGADVLGIDVDEHYLAQARWAKGRFDADRV
                     AFRRASIWEVDRLGRFDLVLFMGVLYHLRHPLLALDLLASTAPGLLVFQTLTHGGEGV
                     AADAGADQGFTSRHRLEDPGWPRMAFIETTFAGDPTNWWIPNHAAVAAMLRSAGFTVE
                     ARPAHEVYICRCGKPAADPDLEAAHRAAVRLEARTDS"
     gene            12339..12527
                     /locus_tag="P5558_03725"
     CDS             12339..12527
                     /locus_tag="P5558_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HRY23452.1"
                     /translation="MRKSAAGLPAGAADDAAPSLRRELATFLILAFVVLPALLFGAVA
                     AYGFVVWFLQILYLGPPT"
     gene            12601..13827
                     /gene="torC"
                     /locus_tag="P5558_03730"
     CDS             12601..13827
                     /gene="torC"
                     /locus_tag="P5558_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HRY23453.1"
                     /translation="MKRLLAFIRDLFLRPSARIGLGVLVVGGFVAGAVAWQGFNTAME
                     ATNEEAFCLTCHTMRDNLLPELQKTVHWSNRTGVRARCPDCHVPHEFTDKVARKMQAS
                     REVLGQLVGTIGTREKFNEHRIVLARREWRRLNANGSRECRACHDYKDMDFDRMRPAS
                     QVAMRAAAERDQSCIGCHKGIAHQLPDLKGEHNPAFDTLVSAAAGQGVEEGDEYYAIL
                     PRPLYRDAALTQPVGTIEVAARVKVLETQGDAQRVELELWRKNKGYGRVWYSHFGLNI
                     VSAILEKEISRDEDLVTVLEAREDPLTGLEWQQVKAEVWLAAESLLDSPEEIWTIARS
                     SYNESCSTCHRQPDEAHFDANAWPGLFAGMVGFTSLDDDTAKVVLKYLQTHSADFRGT
                     AHGADPTLGVVQTARP"
     gene            13841..16360
                     /gene="torA"
                     /locus_tag="P5558_03735"
     CDS             13841..16360
                     /gene="torA"
                     /locus_tag="P5558_03735"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF011682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HRY23454.1"
                     /translation="MSFPRRDFLKGMAAISGTGLVAPSLLTPSPGKAQGAGVEMTDDI
                     TTWKITGSHFGAIRAKVVNDRVLEVQPLELDQHPTEMIKGIIGLIYSPSRIRYPMVRL
                     DWYRNRHKSDTTRRGDNRFVRVGWDEAIDLFYAELERVQQEHGPWALHTAGVGWRSVG
                     QMHSCGNHMVRAIAMHGRSVGTIGDYSTGAGQVILPYVLGSTEVYSQGTSWEVILEQS
                     QVIVFWANDPIKNLQVGWNTETHEAYAYYAQLKDKIARGEIRVISVDPVRSKTQNYLE
                     CEHQYVNPLTDVPLMLAMAHTLVTEELYDAEFLETYALGFDRFVPYLEGKTEDMVEKT
                     PEWAEAICGVPAERIRELARLMAGNRTQLIFGWAVQRQQHGEQPYWMGAVLATLLGQI
                     GLPGGGISFSHHYSSVGVSSSGAAMPGAFPLNLDPGRTPKHDNTDYQGYSAVIPISRA
                     IDALLEPGKEIDFNGAKVKLPPYRMGVFIGHNQWHRQQQRNRMKEAYHRLETVVAIDY
                     NWTATCRFADIVLPACTPFERNDLDAYGSYSNRGVLAMHKLVDPLFNSRPDFAIFSAL
                     CRRFNRENEYTRGMNEMQWIEHLYNECVAENMLKDMPMPPFAEFWEKGYHLFPEGEPW
                     VRHASFRDDPEVNALGTPSGFIEIFSRKIERYGYADCKGHPIWMEKIERSHGGPGSDR
                     FPLWLQSVHPDNRLHSQLCESEPLRETYAIQGREPIFMSPEDAAARGIAHGDIVRVFN
                     DRGQLLAGAHVSDDFPPGVVRIQEGAWYGPTGPEIGALDTYGDPNTLTMDIGTSSLAQ
                     APSANTCIVEMEKFVGTAPAVTAFGGPIEVDPDGNPIKGDA"
     gene            16353..17105
                     /gene="torD"
                     /locus_tag="P5558_03740"
     CDS             16353..17105
                     /gene="torD"
                     /locus_tag="P5558_03740"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HRY23455.1"
                     /translation="MPESRSEDVEAPLSPGEWATVSEQRALVYGWLSTLYAAEIPQAR
                     LAAYLAGEAAPLLEGFATMGLGAEAGRLQAAIDALREVRDAHLELAADFAQLFLLDAS
                     AGALPYASAHDAGQGHLFGPAEARMRAFLARASLAIRDDFKEPADHLAVHLALMARIA
                     QEQAGAADVAEAARDQAAFLRDALLPWLPSFAARSQRASPRLDLYPALAALLLALVEH
                     DAGFLGDVAASFARGGPSPASCGSPSRRRRSG"
     gene            complement(17044..17982)
                     /locus_tag="P5558_03745"
     CDS             complement(17044..17982)
                     /locus_tag="P5558_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRY23456.1"
                     /translation="MPRQHELPATPENIAWGYIDAARPAVLRVASGDTVRLASWAAAR
                     EADLPPDRSLVQAGHLEALHRCPPGPTSHMITGPVHVEGAEPGDVLQIDILEVTPIDR
                     WGWTAIKPLLGTLSDEFDNLSIIHPLIDLEGGTARLPWGRDLPLDPFFGVVTVAPPPH
                     WGRCSSMQPRKFGGNLDNKELRAGSTLYLPVFNEGALFSAGDGHGMQGDGEVCVTALE
                     TGLSGSFRLTVRKDLDYAYPFAETASHLISMGLHAELDEAARIAVREMIAHIRRRTSL
                     SREEAYMLCSLAADLRVTQTVDGEKGCHMMLAKAHL"
     gene            complement(17995..18888)
                     /locus_tag="P5558_03750"
     CDS             complement(17995..18888)
                     /locus_tag="P5558_03750"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY23457.1"
                     /translation="MRIEIALDAGNGVGEGPFWDDVENVLRWVDISKKRVHRWHPATG
                     ARRHWQLDDFPSAVVPCEQRQGLLLAMRDGVYLMDEATGALELFVAHEPDRPENRTNE
                     AKCAPDGSFWVGTMQNNLHPDGSPKPMTGSTGALYRIRHDGGITREVDHVGLSNTLAW
                     TDEGRTLLFADTLTGVISAWEVRDDGRLGASRVFSDEKLPGYCDGSAIDAEGYLWNCR
                     FAGSSIVRFAPDGSVDRIIELPVSQPTSCCFAGPDLATLFVTSARHGLDAAALARNPA
                     EGAVLAIETDVRGTPSIRFAG"
     gene            complement(18963..19358)
                     /locus_tag="P5558_03755"
     CDS             complement(18963..19358)
                     /locus_tag="P5558_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY23458.1"
                     /translation="MLSHVTIGTNDLKRAKAFYDRIMAVLRIPCIADEQLNGLVGYAE
                     SRQTTPQLYLMRPIDGRPAAVGNGQTFAFLAQDRATVRAFHQAALAAGGTCAGPPGLR
                     AHYHPDYYGAYVRDPDGHKLACACHLPED"
     gene            complement(19379..20743)
                     /locus_tag="P5558_03760"
     CDS             complement(19379..20743)
                     /locus_tag="P5558_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRY23459.1"
                     /translation="MPRIDGKTLIEMGYEPGPWFKAALLAALAEPEAAELRPVIDRVR
                     DAWLAAERKKIDDARAQARKALPAGVVHDPGEAAPAPVVVGEHDAGTVAQMNRCLAYG
                     SVLGGAICADGHLGYAQPVGGVIAYREHVSVSGVGFDIACGNMAVRTDLATADLGDRD
                     ALADRLARSLSFGIGRNNAETVEHALLDDADAWREGDVEDLRQMAGEQLGTIGSGNHY
                     VDLFADPEDRVWIGVHFGSRGFGHKAATRYLQMAGGKEGIDVPPTVLHVESEIGRRYL
                     AAMRLAGRYAYAGREWVVGKVLELIGGEAVETVHNHHNFAWEEDHGDAGKAWVVRKGA
                     TPAFPGQRGFVGGSMGDDAVIVEGVGGAASKAIWRSTIHGAGRTFGRREAIRRFARPE
                     MDAWLKERRVTLRGAGLDESPMAYRRLDAVLAHHADTIRVVTRLRPLVVVMAGAGEVD
                     PYKD"
     gene            20877..>21127
                     /locus_tag="P5558_03765"
     CDS             20877..>21127
                     /locus_tag="P5558_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23460.1"
                     /translation="MLETALKLALKSTLAGGVAALALLSWSGAAQAQERYTYLHHATP
                     TNVFWQAVKKGMDEACGQIEADCQMVFLQQDGNFQEQLN"
     assembly_gap    21128..21227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21228..22021
                     /locus_tag="P5558_03770"
     CDS             <21228..22021
                     /locus_tag="P5558_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY23461.1"
                     /translation="CQMVFLQQDGNFQEQLNNLEAVIAQDPDGIIMTFAGGNIFDEAI
                     DRAMEAGIPVLASNVDHPDKTHRLSFTGQDLEQAGYDLAAGLAPQFPAEGPIHVLIGI
                     SGPGQVWAESRGAGIARFMDDYIADNPGREITYEKIDSGLDLAITGQRVAAYVQTTPT
                     TAYFDVGYWAAGAAVSLRDLGKEPGEVLLACFDLVPVVLDEMKTGYIQLTIDQQPFLQ
                     GYVPIHQLHMMNKYKLGAYDANTGKALVTPDQVDALLELSAQGIR"
     gene            22090..23049
                     /locus_tag="P5558_03775"
     CDS             22090..23049
                     /locus_tag="P5558_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23462.1"
                     /translation="MQSILKTLLSRPEISALVMLVVVVAGFSFMAPQFASYGNTRVLL
                     FSLPELGIVVLGVGILMIVGEFDLSVGSVFALVPMLMVIAMGRWGVPPIVAMLMGFAL
                     ALAVGYINGWITLTFNIPSFVTTLGMLFMARSLATVLSGGFPPPFPANLSTDLFVADL
                     GLFRSSMLWFAGFALILGIVLHFSNLGNWIFATGGQSQAAADMGIDTRKVKLFCFMLC
                     SFLAGFAGMITTLRLRSALPALGEGLELQAIAAAVIGGAALTGGIGSLVGMLVGTTLI
                     RVIDNGLVMARIDANWFRFAIGALTIVAVILNTWVRARARRMK"
     gene            23060..23851
                     /locus_tag="P5558_03780"
     CDS             23060..23851
                     /locus_tag="P5558_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY23463.1"
                     /translation="MTARLEEHFQATETFVRPTAEALVRCEGLEKWYGSVHALAEVDF
                     HAEPGEIVGLVGDNGAGKSTLIKILSGAHPADGGRIFFEGREVRLDSPKAAMGLGIET
                     IYQYTAMVPAMSIARNMFIGREPLTRFTIGGIGLMDRRTMVEHATRSLTNIDLTERSP
                     DTTVEELSGGQRQAVAIARAMYFKSKLLILDEPTNHLSVKETNKVLDYVASLKEQGIG
                     SIFISHNLHHIFPISDRIVAMARGRKIADIKKSDTTIDDLTDLIV"
     gene            23877..24635
                     /locus_tag="P5558_03785"
     CDS             23877..24635
                     /locus_tag="P5558_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23464.1"
                     /translation="MEHKGRTALVTGATGGLGVAESKGLALAGANVLMLDVKGREAAE
                     ALAGELPEGAGAVRFVGCDLADPEAAQDLVRLLDAEVGGIDILVNNAAINPLKPVDGY
                     DLAEWRKVQDVNATSAVALAQAVVPSMKKKGWGQIIQICSVTLNGGWSDFTSYVSSKG
                     TLLALTRSLARELGQFGIRVNAVSPGAIPTALEKEVWADQLETYVKFLVDHQALKYRG
                     SPEDIAEAILFLVSDKARFITGQNLSVDGGWWMH"
     gene            24623..25378
                     /locus_tag="P5558_03790"
     CDS             24623..25378
                     /locus_tag="P5558_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY23465.1"
                     /translation="MDALTGAMPEAISEPRRSYPRHGVHGRLVEAIGRLIIGGGLAAG
                     AVLPREVELMDEFGSSRAAVREAVKVLAAKGLVETRQRRGMRIRALEEWNLLDPDVLA
                     WCAASGPAPLLMAQMVELRCLIEPRAARLAAGRRTAAELEALEAAHAQMVATFADAVA
                     YNLADLAFHRAVFRACGNPFVDRLGAIVAVVLEGGFRLRDGGMVPIEAGLVAHGRVLA
                     AIRDGEAAAAEAAMLAVIEVARRELPPVDGGPC"
     gene            25517..25723
                     /locus_tag="P5558_03795"
     CDS             25517..25723
                     /locus_tag="P5558_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HRY23466.1"
                     /translation="MNEDVFNMELRKFLKHVGVTSQREIEQAVRDALAAGKLKGDERL
                     RARMVLTVGGLDLEHEITSDIALK"
     gene            25720..27027
                     /locus_tag="P5558_03800"
     CDS             25720..27027
                     /locus_tag="P5558_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23467.1"
                     /translation="MTGGFARTLLALITLLAGAGPVAAAFIGHGGPVRGLAVTADAAT
                     LVSASFDYSVILWRLADGAASEVLHGHDAAVNAVALLPDGSGFVTAGDDAAVLVWRFG
                     ERAPVRRLIGHTGRVVALDVAPDGRRVASAGWDRTARVWDVETGAELLRLEGAANVDA
                     VRFTGDGATIVTAGADGAVRLWRAADGTALAVLGGGPVGLNALVLGRDGLAYAAGADG
                     AVAVWDLTSGERRAALSTGVEAPLFALAISSDGRLLAAAGMAGTITLFDLASGEIAAV
                     LAGERQPLWSLAFSPDGATLYAGGNDRTIRQFSVAEGVEIEAPAALPPMEVAATNGDA
                     GALAWRRCVACHTLTPDGGNRAGPTLHGIFGRRIGTVAGYPYSPALAGGDLVWTEETV
                     GRLFALGPDVVTPGTKMPIQRIPDAAERAALIGFLKREAMGGE"
BASE COUNT         3820 a         9276 c        10111 g         4124 t
ORIGIN      
        1 ggcgagggta cgcaactcgt cgccctcgtg caccacggca ccgacatgga ggatgcgggt
       61 gccgccgcca tcggtggtgg cggtcgcggt gccgcgcagc caggcgacga cgccggcctc
      121 ggccgcgcgg gcggtgccgc cgcgggcgag caccgccacg gccaggacga agccgaggcc
      181 tcgctgcaag ccccactgga ggcaacggcg gcggttcgga cgccaggggg gcggaggggc
      241 ggccatggct ggatcgtggc tcagagtcgc tcgacctgca acagctcgcg cgggccgacc
      301 caggcccggt cgaggaggtc ggccgtgacc ttggcacccg cctcgtcgcc catggcgcgc
      361 tgcgcctcca tcaggccgaa gagcgaccag ccattgttgg gggtggccat gagcgcatcc
      421 tggaagtgcc gggatgcggc ctcgggctcg ccggccatga gctcgaccgc ggccagggtc
      481 tgcaccaccg ggtagtgcca gtagggcggc tccatgtagg gcacggtcgc ctgcagggcg
      541 acggcctcga gcagccggct ctcggccgtg gcgaagtcgc cggcggcgac ggcgatcctg
      601 gcgcggacga catgggcggc gagggcgagc aggtccgggg cggggacata ttgctcgacc
      661 aggagctgga gttgcgggtc cagcgccagg gcctcgatcg cggcggcttc gccggcggcg
      721 gcgtcggtgt cgccggcgcg ggcgagggcc acgcccctgg cgtagtgcca gaggcccttg
      781 acgaagggca tggcgtcgcc cggatccggg agggccatga cggcatcgag ctcgccgagc
      841 tgggccgtcg cggtgaaggg ggcggtcctg atcgcctgga cccaggcgac ctccgccgcc
      901 acctcgtcgg acatgacggc ggcgagcttg tgggcggccg ccatggcgga gatggcgtcg
      961 cccgccatgg tggcgctggt caggacgaag tggacgttgt gcgggtagta gccgaaccgg
     1021 tagaggcccc cttcctcccc ggcggcctgc cattcggcga agagcgcctc gtcggcctcg
     1081 gcggcggcga tgttggtggc gagcgaatcc tggtagcggc cgacgcggta gtagatgtgg
     1141 gccggcatgt ggacgacgtg cccggcggtc ggcatcaggg cggccagccg gtcggcatgc
     1201 ggctcggcgc gcgccgggtc ggaagaggcc tcgacggtgt ggatatagag gtggatggcg
     1261 tggggatgat cggggtcggc ggcaagcgct gtctcgagca gggcgaccag ccggccggcg
     1321 cggcccttgg gcgtgacccc gtcggcctcc cagtagtccc agggctggct gttcatgacc
     1381 gcctcggcgg cgagcaccaa gagctcgacg tcgtcggggt gggcgtcggc ggcggccagc
     1441 atggcgtcgg cgtaggcctg gtcgagggcg gcgcggtcgg cgtcggcggc catgctgtag
     1501 cgggtcttca gcgcccggat cagcgcctgc tcgcgcgggc tcgcaccctc gatgagcagc
     1561 gtcgccatgg ccatggcctg gaaggccggc cggacggcat cggcatgcat ggcgtcgttg
     1621 atgttggggc cgagcgcgta ggcctcgccc cagtagcaga tggcgcagcc gggatcggcc
     1681 gcctgggcag cgcggaaata cgcggcggcg agccggtggt tgaagcccat gagccaggca
     1741 tagccctggg cggccagggc tctggcctcg tcgctcctgg tggtcgcctg gagccggtcg
     1801 gggccgaggc cgtcgaggat cggtagtggc gacccgggcg acgcctcgac ggcggcagcc
     1861 gggctggcga gcgccgcgag ctggacgccc ggcttgaaga gctcgttcct ggcgtagcgc
     1921 agcccgccgc aggcgctggc ggcgaccctg gccgggtcga gcagctcgct gatgtcgggt
     1981 gtcgcgggtt gggcaaaggc gctgccggcg agaagggcgc cggcgagcga gccgaggccg
     2041 agcctcgacg cggaagcgat cgtcgtcttc gccatgggtt tcctccccct tgcggaatgg
     2101 cccatttctg ttccgatcat agcacgcccg gctgtgaccg ccgctacacg aacccgaggg
     2161 aacgcggctg cgcatcgaac gttctggcgt ccacgacggc agcaatgtcc gggactgcgg
     2221 gagaacggcg atgagcgagg ccacgacgac gaccgatcat gccacgatcc ggcgctgggt
     2281 cgaggcgcgt gacggtcggc cggccagggt gaaggcgacc gccgggggcg agcccggcag
     2341 cggcagcggg ggcatcctca ggatcgattt ccgggagccg gacgaggcgc tcgaggagct
     2401 ctcctgggag gcgttcttcg agatcttcga ccaaaaccgg ctggccttcc tgcaccagga
     2461 caagacggcg gacggctcga ccagccgttt cttcaagttc gtcgagcgct gagacggcat
     2521 ctcctcgaag ggtgggcgga acatggtgtc cagggttctg gtgaccggtg gcgccggctt
     2581 catcggccgg cgggtggtgg cggcgcttct ggcgcgcggc cacgaggtgc gggtgctcga
     2641 tgcgctcatc gagcaggtgc atggcggcgg acggccggcg ctggccgatg tcgagctgcg
     2701 ggtcggcgac gtgcgtgacg gcgaggtggt cggcagggcg ctcgccggca tggacgcggt
     2761 cgtacatctg gcggccgagg tcggcgtcgg gcagagcatg tacgagatcg agcgttatgt
     2821 cggggtgaac gagctcggca ccgccacgct gttccagaag ctggtcgagc ggccggtcga
     2881 gcgggtggtc accgcctcct cgatgagcgt ctacggcgag ggcctctatc gtgagcgggg
     2941 cggccgtatc cgcgccgacg tcgagcggcc gcggccggcg ggcggcggct gggaccctct
     3001 cgacggggct ggtctgccgc tggtgccggt gccgacgccg gagtggaagg cgccgtcgct
     3061 ggcctcggtc tacgccctca acaagtacgc gcaggagcgg ctcacacatg tcgtggcgaa
     3121 ggcctacggc atggccggcg tggcgttgcg gctgttcaac gtctacggct cggggcaggc
     3181 gctctccaac ccctacacgg gcgtgctcgc gatcttcgcg gcccggctgc tcaacggcca
     3241 ggcgcccatg gtgttcgagg acggcgagca gcggcgcgat ttcgtccatg tggacgacgt
     3301 ggcggaagcc ttcgccctgg cgctggagac gcctgccgcc gccgggcgga ccttcaacat
     3361 cggcagcggc gtcgacgtct cgatcgccga ggtggcgagc cggctcgcgg ccgcgatggg
     3421 gcggccggac atccgcccgc tgatcaccgg caaggcgcgg gccggcgaca tccgccattg
     3481 cttcgccgac atcggcgccg cgcgggaggt gctgggctac gcccccagga cgggcttcga
     3541 cgaggggctc gccgaggtgg tggcatgggt cgcacggcaa cgggcggtgg accgggtcga
     3601 ggaggcgcgg cgcgagctcg aggaacgggg tctcgtggca tgagcgccgg gctgggcgtg
     3661 gtcgaatggt tccgaccggg cgaccattcg cgggtcgagg cgagcctcga gcggctgcgc
     3721 cggctcggtt gcagccgcct gcgcacgcat ctttcctggg cggactacca tgccgagggc
     3781 gggcgggact ggtacgactg gctcctgccg aggctggcgg gcgaggtcga gctcctgccc
     3841 tgcctgcact acacgccgcc ctcgctctcg gagaccgggg cggtcgccgg cccgccgcgc
     3901 gacctcaagg cgttcgccga cttcctcgac cacgtggtcg agcggcacgg tcgccatttc
     3961 gccgcggtcg agctctggaa cgagccgaac aacctgctcg actgggactg gcgcgtcgac
     4021 accgactggt cgaagttcgc gaccatggtc ggggccgcgg cccattgggg gcggcggctc
     4081 ggcaagcagg tggtgctggg cggcccctgt cccgccgaca tgaactggct cgagctcatg
     4141 ggccggcggg gcgtgctcgg cgagatcgac gcgctcggcc tgcacggctt tcccggcacc
     4201 tgggattccg aggcggcggg ctggcccggc tggaacagcc tcgccgagga ggcgcttgcg
     4261 gtgctggacc ggcacaaccc ggccgtcgag ctctgggtga cggaagccgg ctacgccacc
     4321 tggcggcacg acgaggcgct ccaggcgcgg gccttcctcg agctgcaccg ggcaccgttc
     4381 gcccgcgtct actggtacat gctgcaggat ctcgccgacg aggtggccgt ccaggaaggc
     4441 ccgcgcttcg acgagcgcca ctaccacatg gggctcttcg acaaggccgg ccggcccaag
     4501 ctgctggctc gcctgctcgc cgagggcggg cccgcgaagg tggcggaggt gatcgagcgg
     4561 gcaccgccgg cgccggccat cgtcggcacc cggccggtgc tggtgaccgg cggggccggc
     4621 ttcatcggct cgaacctcgc cgaccggctg gcggcggagg gcgagcatgt gctggtgctg
     4681 gattccctgg cccggccggg ggtcgagcgc aacctcgcct ggctcgagca gcgccaccgg
     4741 cggcacgtct cgtcggcgct ggtcgatctg cgcgaccgcg acggcgttgc cgccgcggcg
     4801 gccgacgcgg ccgcggtctt ccacatggcg gcgcagacgg cggtgacgac gagcctcgtc
     4861 gaccccttcg gcgatttcga tgtcaatctc gccgggacga tcaacctgct ggaggccgtg
     4921 cgccggcgac cggtgccggt gatcatggcc tcgaccaaca aggtctacgg cgatttgggc
     4981 aatgtcgagc tgatccggga aggggagagg tggctgccgc gcgatcccag gcttcgctgt
     5041 cacgggatcg acgagacgcg cgccctctcg ttccacacgc cctatggctg ctccaagggt
     5101 gccgcggatc agtacatgct cgactatgcc cggagcttcg gcgtgccgac ggccgtcctc
     5161 cgcatgagct gcatctgcgg cccgcgccag ctcggcaccg aggaccaggg ctgggtggcg
     5221 catttcctca tccgcgccct ggagggccgg ccgatcacca tcttcggcga cggccggcag
     5281 gtgcgcgacg tgctgttcgt cgacgacgcc gtggccgcct atctcggcct ctggcgcgcc
     5341 atcgacagga tcgccggccg ggcgttcaac ctgggtggcg gcgccgccaa cgcggtcagc
     5401 ctgcggcagg tgctgcgggc gatcggcgag ctcgccggtg ccgaggtcga ggtcggctac
     5461 ggcgactggc ggccgggcga ccagcgctgg tacgtctccg acacgcgctc cctcagccgg
     5521 gcgatcgacc tgccgcggcc gctcgggtgg cgttccgggc tcgagcggct ggctcggtgg
     5581 ctgcgcaccg atgggcgcga cgccgaggtc ctggcgtcgc cggtggcgga ggtcgtctga
     5641 tgcgggtggc cctggtcaat ccccgctggg atttcacgaa cagcatctat ttcggctgcc
     5701 ccgagtcgca tctgccgctg gagctcggct atgccgccca gcgcctcgag gaggcgggac
     5761 acgaggccct gctgctcgac gggcagctcg acgggctcga gaacgaggcg ctggcggacc
     5821 gggtggccga tgccggggcc ggcctgaccg tggtgacgac cgcacccacc tatctcttct
     5881 ggcgctgcgc ccagccggag ctgcgcacgc cccgtgcctt cctcgatgcg ctgggcggcc
     5941 ggggcgggcg gacggtggcg gtggggccgc acggctcggt gacaccggcg gcgacgctcg
     6001 agaagctcgg cgtcgacctg gtcgtgcgcg gcgagtgcga ggaggtgatc gcccggctcg
     6061 ccgacgccgg cggcgagcct tttggcatcg ccggcaccgc ctggcgggac ggcgacgatc
     6121 tcgaacggga gggcgggccg gtcgcggcga cgttcgtcga ccagaaggcg ctcttctggc
     6181 cggacgcctg gatcgcccgt catgcccacc accaccatcg cttcgacgcc aggcccgacg
     6241 tgccgggtgc cgaggtcgag gcgtcgcgcg gctgtcccta cagctgcagc ttctgcgcca
     6301 agctggacta tcgcgacaag taccgccgcc gcgagctcgg cccgctcatg gaggagatcg
     6361 accggctgat cgcgcagggc gtgggctaca tctatttcgt cgacgagatc ttcctgccgc
     6421 agaagcccct gctcgaggcg ctggcggccc gcgacatcgt cttcggcgtc cagacccgga
     6481 tcgatctctg gtcgccaccg atgctgcaac tcctgggcga ggccggctgc gtctcgatcg
     6541 aggccggggt ggagagcctg acaccggagg gtcgcgacgc cctcgacaag aagtgccgca
     6601 tggcgaccga cgagctggcc gagcggctga tcactgcccg ccgcttcgtg ccgttcgtcc
     6661 aggccaacct gatcgccacg gcgggtgacg accccgctct cgtcgccctc tggcgcgagc
     6721 ggctgcgtgg cgccggtgtc tgggccaacg acccggtgcc gctctacccc tatccgagtt
     6781 cgccggacta tcacaagcgc tggggtgcgc cggacgatgc ggcctgggag cgggcgcacg
     6841 agcattacct cgcccagttc gagcggttca gcgacatcca ggaccagcgc ccgctgcccc
     6901 tgccggagct ggaggcggca tgccgggggg cgtgacggag aaacggcacc gcctgctcct
     6961 gaccacggac gcggtgggcg gggcctggac ctgggcgctc gatctcgccg ccgccgccat
     7021 cgcccggggc tttcgggtgc ggcttctggt cctcggcccg ccgccctcgc cggatcggcg
     7081 gcggcaggcg gcggccgtgg ccgggctcga gctggtcgag acgggcctgc cgctcgaatg
     7141 gacggcggcg acgccggagg aggtgatcga ggcgtcgcag caactggcct cgcaggtcga
     7201 aaggcagggg cctgacctcg cccatctgag caacccggcg ctcgccgccg ccggtccctg
     7261 gcccgtgccg ctggtcgcga ccctgcacag ttgcgtcggc acctggtggg ccgccatgca
     7321 ccctggccgg gcgatgcccg atgacctgcg ctggcggcag gcgctcgtcg gccggggcct
     7381 ggccaatgcc gacatcgtga ccgtgccgac cctcggcttc gcccaggcgg cgagcgagct
     7441 ctacccggac atcgtcttcg aggtggtgca gaacggccgc cgcgcggacg gggaggcgcc
     7501 gatgggccgg ccgcgggcgc gggtgctcgc cgccggtcgc ctctgggacg aggccaagaa
     7561 catggcgacg ctcgacgccg cggccgcccg gcttgccgtg cccgtcgagg cggccggccc
     7621 ggtcgtgggg ccgaacggcg ccaggatcga gctgccgcat ctccgccttc tgggcgagct
     7681 cggggcgggc gcgatgcggc agaagctggc caggacggcg atcttcgtct cgccggcgcg
     7741 cttcgagccc ttcggcctcg gcgtgctcga ggcggcgcag ggcggtgccg cgctgctgct
     7801 ctccgacata gcggtgcatc gcgagctctg ggacggtgcc gcgcagttct tcgaggctga
     7861 ggatgccgtg tcgctggcgg gtgccatcgc ggcgctgatg gacgacccgg cccgggtcga
     7921 gcggctcgcg gcggcagccc tgtcgcgagc gcaggcgttc ggtgtcgatc gcatggcggc
     7981 cggcatgatc gccctctacg agcgcctcct ccggcacccc ttggcgcttt ctggtgccgc
     8041 atgaacatcg cgtacctgac ccattccctg gcctcgtgct ggaaccacgg caacgcccat
     8101 ttcctgcgcg gtctccttcg ggagctgggg cgacgcggcc atcgctgcat cgcctgggag
     8161 ccggaagggg catggtcgct cgaccatctc gtgcgcgatc acgggcccga ggcgcttcgg
     8221 gccttcgccc gcacctttcc cgaccttcgc cgggcgacct acgaggacgc ccaggcggcc
     8281 gagcggatcg tggacggcgc cgacctggtg atcgtgcacg aatggaccgg cccggccatg
     8341 gtcgcggcgc tcgggcgcct gcgcgcccgg ggcggccggt tcaccctcct cttccacgac
     8401 acgcatcatc gggcggtgag cgatccgggg gccgtggcgg cgctcgacct cgaatccttc
     8461 gacggggttc tggcgttcgg cgagagcctc gccgaagtct atcggcgccg ggggtggggg
     8521 cagcgcgcct tcgtctggca cgaggcggcg gacacgacgc tcttccggcc gccggtggtc
     8581 gcggggccac gcgagggcct cgtctggatc ggcaactggg gcgacggcga gcgcacggcg
     8641 gagctcgact ccttcctctt ccggccggcg gcgagccagg gcctcgccct cgacgtgcac
     8701 ggcgtgcgct acccgccgga cgcgctggag cggctggcgg cgcatggctt tcgctaccgg
     8761 ggctgggtcg ccaacgccga ggtgccgggc cgtttcgccc gccacctcgc caccgtgcac
     8821 gtgccgcgcc gcttctatgt cgagcatctc ccgggcattc cgacgattcg cgtcttcgag
     8881 gcgctggcct gcggcattcc cctgctctcg gccttctggc cggacacgga ggcgctgttc
     8941 cgcccgggca ccgaccacct cgtggcgcgg agcggtgccg agatggcggc gcacatgcgg
     9001 gcgatccgca acgatccgga gcttcgggcc ggcctcgcgg cggcggggct tgcgaccatc
     9061 gaggcgcggc acacctgcgc gcaccgggcg gacgagctcc tcgccatcgt cgagcggctg
     9121 cgcggggcgg ccacgcgggt ggaggcgtga tgcgcatcgc cttctacggc tccagcctcg
     9181 tctccgccta ctggaacggt gccgccacct attaccgcgg gctcctgcgc catctggccg
     9241 cgctcggcca cgagatcacc ttcctcgagc ccgacgcctt cggccgccag gagaaccgcg
     9301 acatcgagcc gccggactgg gcgcgggtgg tcgtctggga ggcgacgccc gaggggctcg
     9361 aggtggcgct cggccatgcg gccctcgccg acctcgtggt caaggcgagc ggcgtcggcg
     9421 tcttcgatga cgcgctcctg gccggcgtgc tcgagcggtc gcggccggag gccgtccggg
     9481 ttttctggga tgtcgacgcg ccggcgacgc tggccgccat gagggagacg ccggaccatc
     9541 cgctgcgccg ggccttgccc tcgctcgatg ccgtcttcac ctatggcggc ggtgatccgg
     9601 tggtgcgcgc ctatcgcggc ttcggtgccc gcgtctgcga gcccgtctac aacgggctcg
     9661 acacgacgac gcatttcccc gtgccggcgg acgcgcgctt tcgggccgac ctcgcctttc
     9721 tcggcaaccg cctgccggac cgggaagcgc gggtccatgc cttcttcctc gaggcggcgc
     9781 ggcgcctgcc cgggcaccgc ttcctgctcg gcggctcggg ctgggatgat gtcgacctgc
     9841 cggccaacgt ggcccggctc ggccacgtgc cgacgaggga ccacaacgcc ttcaatgcca
     9901 cgccgctcgc cgtcctgaac atcgcccgcg acagcatggc gcaagtgggc ttctcgccgg
     9961 cgacgcgcgt cttcgaggcg gcgggtgccg gcgcctgcct gatcaccgat caatgggagg
    10021 gcatcgagat gttcctcgag cccggcaccg aggtgctggt ggcgcgggac ggggcggagg
    10081 tggcggcgca tctggcggcg ctcgatcgaa agcgggcggc ggcgatcggc cggcgggcgc
    10141 tcgcgcgggt gcgccgggcg cacagctacg agcgccgggc ggcggaggtc gacctgcacc
    10201 tgcggcggct ggtggacgac cggcggcggg tcgacgcatg aggatcgtga tcctcggcct
    10261 cagcctctcc tcctcctggg gcaacggcca cgccaccacc taccgggcgc tggtcgaggc
    10321 gctgcacgcg cgcggccacg aggtggcctt cctcgagcgg gatgtaccct ggtatgcgga
    10381 gaaccgcgat ctcgaccgct cgccgcactg cgatctccac ttctaccaga cgctcgacga
    10441 tctcgcggcg ctgcggccga cgatcacggc ggccgacgcg gtggtgctgg gctcctacgt
    10501 gcccgagggc gttcgcgtcg cggagcatct cctcgccgtc gccgaagggc cggtcgcctt
    10561 ctacgacatc gacacgccgg tgacgctcgc caagctcgag gcgggcgatc acgagtacct
    10621 gacgccggcg ctgatcccgc gcttcgatct ctacctctcc ttcagcggcg gtgccgcgct
    10681 cgaggccctc gtcgggcgct ggggggcgag gctcgcccga ccgctctatt gcggcgtgga
    10741 tgcggagcgc taccgcccgc tggcgctcgg gaagcggtgg gacatgggct atctcggcac
    10801 ctacagcgcc gaccgccagc cggcgctcga gcggctgctg ctggagccgg cgcggcgcct
    10861 gcccgagcgg cgcttcgtcg tcgccgggcc gcaatatcca cgaggcatcg actggcccgc
    10921 caatgtcgag cggatcgagc acgtgccgcc gaccgggcac gccgccttct acggagcctt
    10981 gcgcttcgcc ctcaacgtga cgagggcggc gatgatcgag gcgggccaca gcccgagcgt
    11041 gcggctcttc gaggcggccg cctgcggtgt gccgatcctc accgatcgct ggaccggcat
    11101 cgaggacttc ttcgcgccgg agcgggagct tcgcatcgtc gacgacaccg ccatggtggt
    11161 cgagacgctc gggggcatgg cggccgagcg ggcgagggcg atgggtgccg ccgggcgcga
    11221 gcgcgtgctg cgggaccata ccgcgtcccg ccgggccgag gagctcgagc ggagcctcgc
    11281 cgtggcggcc gggaggtagg cccgatggcg gatctggcgg acgcgatcgc ccggctggcg
    11341 ccctggttcc acaacctcca tctgcccggc ggcgtggaga cggcgccgga ccacttcctc
    11401 ggcgacttcc cgcgcttcaa gtggcgggag atcgccccgc atctgccggc cgatctcgcc
    11461 ggtgcccggg tgctggacat cggctgcaac gccggcttct acagcttcgc cctggcggcg
    11521 aggggcgccg atgtgctggg catcgacgtc gacgagcact acctggcgca ggcgcgctgg
    11581 gcgaaggggc gcttcgatgc cgaccgcgtc gccttccgcc gggcctcgat ctgggaggtg
    11641 gaccggctcg ggcgtttcga cctcgtgctc ttcatggggg tcctctacca ccttcgccat
    11701 ccgctcctcg ccctcgacct gctggcctcg acggcacccg gcctcctggt cttccagacg
    11761 ctgacgcatg gcggcgaggg ggtggcggcc gatgcgggcg cggaccaggg cttcaccagc
    11821 cgccaccgcc tcgaggaccc gggctggccc agaatggcct tcatcgagac gaccttcgcc
    11881 ggcgacccga ccaactggtg gatcccgaac cacgccgccg tggcggcgat gctgcggtcg
    11941 gccggcttca ccgtcgaggc gcggcccgcg cacgaggtct atatctgccg ctgcggcaag
    12001 ccggccgccg accccgatct cgaggcggcg caccgggcgg cggtgcggct cgaggcacgg
    12061 acggattcct agcggcgcgc cttcgtgagg caggggtggc gaggcggacg gcgcggtcag
    12121 gcgtgtctgc caaacttcat tttggcccgc tggcgatttc aaccggtcga acattgacag
    12181 ggccatgaag cggcggtgtt atggcagttc tcgacatgtc gatcgatgct ggaagcgaat
    12241 cccggcacga cgttcgacat gcgtcgggac aagacaagaa ggctcgggct cattcgttcc
    12301 atggtcaggg gctcggccgc tcgaggggag gcgtcgggat gaggaaaagc gcggcggggc
    12361 tgcctgcggg cgcggcggat gatgcggcgc cgagcctgag gagggagctc gcgacgttcc
    12421 tgatcctggc cttcgtggtg ctgccggccc tgctgttcgg cgcggtcgcc gcctacggct
    12481 ttgtcgtgtg gttcctgcag atcctgtatc tcggcccacc cacttgaggc tcgagcgggc
    12541 gcggctcggc gagggggcgt gcctgcccga gccatcgcgc cattcgggga gagcgtgacg
    12601 atgaagaggc tcctcgcctt catccgggat ctgttcctca ggccttcggc gaggatcggc
    12661 ctgggcgtgc tggtggtcgg cggcttcgtt gccggtgcgg tggcctggca gggcttcaac
    12721 acggccatgg aggcgaccaa cgaggaggcg ttctgcctga cctgccacac catgcgcgac
    12781 aaccttttgc ccgagctgca gaagacggtg cactggagca accgcacggg cgtgcgcgcc
    12841 cgttgcccgg actgccatgt gccgcacgag ttcaccgaca aggtcgcgcg caagatgcag
    12901 gccagccgcg aggtcttggg gcagctcgtc ggcaccatcg ggacgcgcga gaagttcaac
    12961 gagcatcgca tcgtgctggc gcgtcgcgaa tggcgccggc tgaacgccaa cgggtcgcgc
    13021 gaatgccgcg cctgccacga ctacaaggac atggacttcg accgcatgcg cccggcgtcc
    13081 caggtggcga tgcgggccgc cgccgagcgc gaccagagct gcatcggctg ccacaagggg
    13141 atcgcgcatc agttgcccga cctgaagggc gagcacaacc cggccttcga cacgctggtg
    13201 tcggccgccg ccgggcaggg tgtggaggag ggggacgagt actacgccat cctgccgcgc
    13261 ccgctctatc gcgatgcggc gctgacccag ccggtcggca cgatcgaggt ggcggcccgg
    13321 gtgaaggtgc tggagacgca aggcgacgcc cagcgcgtgg agctcgagct ctggcgcaag
    13381 aacaagggct acggccgggt ctggtacagc catttcgggc tgaacatcgt cagcgccatc
    13441 ctcgagaagg agatctcgcg ggacgaggat ctcgtgaccg tgctggaggc cagggaagac
    13501 ccgctgaccg ggctcgaatg gcagcaggtc aaggccgagg tgtggctcgc ggccgagagc
    13561 ctgctcgaca gcccggagga gatctggacg atcgcccgct cgagctacaa cgaaagctgc
    13621 agcacctgcc atcgccagcc cgacgaggcg catttcgacg ccaacgcctg gcccggcctg
    13681 ttcgccggca tggtgggctt caccagcctg gacgacgaca ccgccaaggt ggtgctcaag
    13741 tacctgcaga cccattcggc cgattttcgc ggcacggcgc acggcgccga cccgaccctg
    13801 ggcgtcgtgc agaccgcgcg gccctgacga ggatgagccc atgagcttcc cgaggcgcga
    13861 tttcctcaag ggcatggcgg cgatatcggg cacgggcctg gtggcgccca gcctgctcac
    13921 cccgagcccc ggcaaggcgc agggcgccgg cgtggagatg accgacgaca tcaccacctg
    13981 gaagatcacc ggctcgcact tcggcgccat tcgcgccaag gtggtcaacg accgggtgct
    14041 ggaggtgcag ccgctcgagc tggaccagca cccgaccgag atgatcaagg ggatcatcgg
    14101 gctgatctac agcccgtccc gcatccgcta cccgatggtg cggctcgact ggtacaggaa
    14161 ccggcacaag agcgacacga cgcggcgcgg cgacaaccgc ttcgtgcggg tcggctggga
    14221 cgaagccatc gacctcttct acgccgagct cgagcgggtg cagcaggagc acgggccctg
    14281 ggcgctgcac accgccggcg tcggctggcg ctcggtcggc cagatgcaca gctgcggcaa
    14341 ccacatggtg cgcgcgatcg ccatgcacgg gcgcagcgtc ggcacgatcg gcgactactc
    14401 gacgggggcg gggcaggtga tcctgcccta tgtcctcggc tccaccgagg tctactcgca
    14461 gggcacctcg tgggaggtca tcctcgagca gagccaggtg atcgtcttct gggccaacga
    14521 tccgatcaag aacctgcagg tcggctggaa caccgagacc cacgaggcct acgcctacta
    14581 cgcgcaattg aaggacaaga tcgccagggg cgagatccgg gtgatcagcg tcgatccggt
    14641 gcgcagcaag acgcagaact acctcgagtg cgagcaccag tacgtcaatc ccttgaccga
    14701 tgtgccgctg atgctggcga tggcgcacac cctggtcacg gaggagctct acgatgccga
    14761 gttcctcgag acctatgcgc tgggcttcga ccgcttcgtg ccctacctcg agggcaagac
    14821 cgaggacatg gtcgagaaga cgccggaatg ggccgaggcc atctgcggcg tgccggcgga
    14881 gcgcattcgc gagctcgcac ggctcatggc cggcaaccgc acccagctca tcttcggctg
    14941 ggcggtgcag cgccagcagc acggcgagca gccctactgg atgggggcgg tgctggcgac
    15001 gctgctcggc cagatcggcc tgccgggcgg cggcatcagc ttctcgcacc actacagctc
    15061 ggtcggcgtg tcctcctcgg gtgccgcgat gccgggcgcc tttccgctca acctcgatcc
    15121 gggccgcacg cccaagcacg acaacaccga ctaccagggc tacagcgcgg tgatcccgat
    15181 ctcgcgggcg atcgacgcgc tgctcgagcc cggcaaggag atcgacttca acggggcgaa
    15241 ggtcaagctg ccgccctaca ggatgggcgt cttcatcggc cacaaccagt ggcaccggca
    15301 gcagcagcga aaccgcatga aggaggccta ccaccggctc gagacggtgg tcgcgatcga
    15361 ctacaactgg accgccacct gccgcttcgc cgacatcgtg ctgccggcct gcacgccgtt
    15421 cgagcgcaac gacctcgatg cctacggctc gtacagcaac cgcggcgtgc tggcgatgca
    15481 caagctcgtc gacccgctgt tcaactcgcg ccccgacttc gcgatcttca gcgcgctctg
    15541 ccggcgcttc aaccgcgaga acgagtacac ccgcggcatg aacgagatgc agtggatcga
    15601 gcacctctac aacgagtgcg tcgccgagaa catgctcaag gacatgccga tgccgccctt
    15661 cgccgagttc tgggagaagg gctaccacct cttccccgag ggcgagccct gggtgcgcca
    15721 cgcgagcttt cgcgacgacc ccgaggtcaa cgcgctgggc acgccctcgg gcttcatcga
    15781 gatcttcagc cgcaagatcg agcgctacgg ctacgccgac tgcaagggcc acccgatctg
    15841 gatggagaag atcgagcgct cgcacggcgg ccccggctcc gaccgcttcc cgttgtggct
    15901 gcaatcggtc catccggaca accgcctgca ctcgcaattg tgcgaatccg agccgttgcg
    15961 cgagacctat gcgatccagg ggcgcgagcc catcttcatg agccccgagg acgcggccgc
    16021 ccggggcatc gcgcacgggg acatcgtgcg cgtgttcaac gatcgcggcc agttgctcgc
    16081 cggcgcccac gtgtccgacg acttcccgcc gggcgtggtg cggatccagg aaggcgcctg
    16141 gtacggcccg accggccccg agatcggcgc cctcgacacc tatggcgacc cgaacacgct
    16201 gaccatggac atcggcacgt cgagcctggc gcaggcgccg agcgccaata cctgcatcgt
    16261 cgagatggag aaattcgtcg gcacggcgcc ggcggtgacc gccttcggcg ggccgatcga
    16321 ggtcgatccc gatggcaatc cgatcaaggg cgatgcctga gagccggtcc gaggacgtcg
    16381 aggcaccctt gagccccggc gaatgggcga cggtcagcga gcagcgggcg ctcgtctacg
    16441 gctggctctc cacgctgtac gccgccgaga tcccccaggc gcggctcgcc gcctacctcg
    16501 cgggcgaggc ggcacccctg ctcgagggct tcgcgaccat gggcctgggc gcggaggccg
    16561 gccgcctgca ggcggccatc gacgcgctgc gcgaggtgcg ggacgcgcat ctcgagctgg
    16621 cggcggattt cgcgcagctg ttcctgctcg atgccagcgc cggcgccttg ccctacgcct
    16681 cggcccacga cgccgggcag gggcatctct tcggcccggc cgaggcgcgc atgcgcgcct
    16741 ttctcgctcg cgcatcgctc gccatccggg acgacttcaa ggagccggcc gatcacctcg
    16801 cggtccatct cgccttgatg gcgcggatcg cccaggagca ggcgggcgcg gcggatgtcg
    16861 ccgaggcggc acgcgaccag gccgcgttcc tgcgcgatgc cctgctgccc tggctgccga
    16921 gcttcgccgc gcgcagccag cgagcgtcac ctcgcctcga cctctatccc gcgctcgccg
    16981 cccttctgct cgccctggtc gagcacgatg cgggctttct cggcgacgtc gcggcgtcgt
    17041 tcgctagagg tgggccttcg ccagcatcat gtggcagccc ttctcgccgt cgacggtctg
    17101 ggtgacgcgc aggtcggcgg ccagcgagca gagcatgtag gcctcctcgc ggctgaggct
    17161 ggtgcggcgg cggatgtggg cgatcatctc gcgcacggcg atccgggcgg cctcgtcgag
    17221 ctcggcatgg aggcccatcg agatcaggtg gctggcggtc tcggcgaagg ggtaggcgta
    17281 gtcgagatcc ttcctgacgg tgaggcggaa ggagccggag aggcccgtct cgagggcggt
    17341 gacgcagacc tcgccgtcgc cctgcatgcc gtggccgtcg ccggcggaga agagggcacc
    17401 ctcgttgaag accggcagat agagcgtgct gccggcgcgc agctccttgt tgtcgagatt
    17461 gccgccgaac ttgcgcggct gcatcgagga gcagcggccc caatgcggcg ggggcgccac
    17521 ggtgacgacg ccgaagaagg ggtcgagcgg cagatccctg ccccagggca ggcgggcggt
    17581 gccgccctcg aggtcgatca gggggtggat gatggagagg ttgtcgaact cgtcggacaa
    17641 ggtgcccagg agcggcttga tggcggtcca gccccagcgg tcgatcggcg tcacctcgag
    17701 gatgtcgatc tgcagcacgt cgccgggctc tgccccttcg acatgcaccg gccccgtgat
    17761 catgtggctg gtcgggccgg gcgggcagcg gtggagcgct tcgaggtggc cggcctggac
    17821 gagcgagcgg tcgggcggca ggtcggcctc gcgggcggcc gcccagctcg caagccggac
    17881 ggtgtcgccc gaggcgaccc tgagcacggc gggccgcgcc gcgtcgatgt agccccaggc
    17941 gatgttctcg ggggtggcgg gcagctcgtg ttggcggggc atggtcggtg tccttcagcc
    18001 ggcgaagcgg atggatgggg tgccgcgcac gtcggtctcg atggcgagga ccgcaccctc
    18061 ggcgggattt ctcgcgaggg cggcggcgtc gaggccgtgg cgggcggagg tgacgaagag
    18121 ggtggcgagg tcggggccgg cgaagcagca ggaggtgggc tggctgaccg gcagctcgat
    18181 gatgcgatcg accgagccgt ccggcgcgaa gcggacgatc gaggagccgg cgaagcggca
    18241 gttccagaga tagccctcgg cgtcgatcgc cgagccgtcg caatagcccg gcagcttctc
    18301 gtcgctgaag acgcgcgacg cgccgagccg gccgtcgtcc cggacctccc aggcgctgat
    18361 gacgccggtg agtgtgtcgg cgaagaggag ggtccggccc tcgtcggtcc aggccagcgt
    18421 gttggagagg ccgacatggt cgacctcgcg ggtgatgccg ccgtcgtggc ggatgcggta
    18481 gagggcgccg gtcgatccgg tcatgggctt cgggctgccg tcggggtgca aattgttctg
    18541 catcgtgccg acccagaagc tgccgtcggg cgcgcatttg gcctcgttgg tgcggttctc
    18601 gggccggtcg ggctcgtggg cgacgaagag ctcgagcgcg ccggtcgcct cgtccatgag
    18661 gtagacgccg tcgcgcatgg cgaggagcag gccctgacgc tgctcgcagg gcaccacggc
    18721 cgaggggaag tcgtcgagct gccagtggcg gcgggcaccg gtggccgggt gccagcgatg
    18781 gacgcgcttt ttcgagatgt cgacccatcg gagcacgttc tcgacatcgt cccagaacgg
    18841 cccctcgccg acgccgttcc cggcatcgag cgcgatctcg atccgcatct ttcgttcctc
    18901 cccgccgctg gcaccgggac tatgggtgct cgcggcgatc gaggcaacgt gctcggggtg
    18961 gctcagtctt ccggcaggtg gcaggcgcag gcgagcttgt ggccgtcggg gtcgcgcaca
    19021 taggcgccgt aatagtcggg atggtagtgg gcgcgcagcc cgggcgggcc ggcgcaggtg
    19081 ccgccggcgg ccagcgccgc ctgatggaag gcgcggacgg tggcgcggtc ctgcgccagg
    19141 aaggcgaagg tctggccgtt gccgacggcg gcgggccggc cgtcgatcgg ccgcatcagg
    19201 tagagctgcg gcgttgtctg ccggctttcg gcataaccca cgaggccgtt gagctgctcg
    19261 tcggcgatgc aggggatgcg cagcacggcc atgatccggt cgtagaaggc cttggcgcgc
    19321 ttgaggtcgt tggtgccgat ggtgacgtgg ctcagcatgg gcgttcggtc cttgccaatc
    19381 agtccttgta cgggtccacc tcgccggcgc cggccatgac gacgacgagc gggcgcagcc
    19441 gggtgacgac gcggatggtg tcggcgtggt gggcgagcac ggcgtcgagg cggcgatagg
    19501 ccatcgggct ctcgtcgagc ccggcgccgc gcaaggtgac gcggcgctcc ttcagccagg
    19561 cgtccatctc gggccgggcg aagcggcgga tggcctcgcg ccggccgaag gtccgcccgg
    19621 cgccgtggat ggtcgagcgc cagatggcct tcgacgcggc cccgccgacg ccctcgacga
    19681 tcaccgcgtc gtcgcccatc gagccgccga cgaagccgcg ctggccgggg aaggcggggg
    19741 tggcgccctt gcgcaccacc caggccttgc cggcgtcccc gtggtcctct tcccaggcga
    19801 agttgtggtg gttgtggacg gtctcgacgg cctcgccgcc gatcagctcc aggaccttgc
    19861 cgaccaccca ctcgcgcccg gcataggcat agcggccggc caggcgcatg gcggcgagat
    19921 agcgccggcc gatctcgctc tcgacgtgga gcacggtggg cggcacgtcg atgccctcct
    19981 tgccgccggc catctggagg tagcgcgtgg ccgccttgtg gccgaagccg cggctgccga
    20041 aatggacgcc gatccagacc ctgtcctcgg ggtcggcgaa gagatcgaca tagtggttgc
    20101 cggagccgat cgtgccgagc tgctcgcccg ccatctggcg gagatcctcg acgtcacctt
    20161 cccgccaggc atcggcatcg tcgaggagcg cgtgctcgac cgtctccgcg ttgttccggc
    20221 cgatgccgaa ggagagcgag cgggcgagcc gatcggccag ggcgtcgcgg tcgccgaggt
    20281 cggccgtggc gaggtcggtg cgcacggcca tgttgccgca ggcgatgtcg aagccgacgc
    20341 ccgagaccga gacgtgctcg cgataggcga tgacgccgcc gaccggctgg gcatatccca
    20401 ggtggccatc ggcgcagatg gccccgccga gcacgctgcc ataggcgagg cagcggttca
    20461 tctgggccac cgtgccggcg tcgtgctcgc cgacgaccac cggtgccggc gcggcctcgc
    20521 ccgggtcgtg gaccacgccg gccggcaggg ctttccgggc ctgggccctg gcgtcgtcga
    20581 tcttcttccg ctccgccgcc agccaggcgt cgcgcacgcg gtcgatgacc gggcgcagct
    20641 cggccgcctc gggctcggcc aaagcggcca ggagcgcggc cttgaaccac gggccgggct
    20701 cgtagcccat ctcgatcaag gtcttgccgt cgatcctcgg catggcggtt tccagggatg
    20761 tcccgaaagg gaacgtgtcc ggccggtcgt cggcctgatc tgatcttgcc ggtcggacgg
    20821 gcggccacta ggctcccggc caacccgctc gagggtcatc ataggggagg tcaacgatgc
    20881 tggaaacggc gctgaaactg gcactgaaat cgactctggc gggcggtgtg gccgccctcg
    20941 cgctcctgag ctggagcggg gcagcccagg cccaggagcg ctacacctat ctgcaccatg
    21001 cgacgccgac caatgtcttc tggcaggcgg tgaagaaggg catggacgag gcctgcgggc
    21061 agatcgaggc cgactgccag atggtcttcc tgcagcagga cggcaatttc caggagcagc
    21121 tcaacaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact gccagatggt
    21241 cttcctgcag caggacggca atttccagga gcagctcaac aatctggaag cggtgatcgc
    21301 ccaggatccc gacggcatca tcatgacctt cgccggcggc aacatcttcg acgaggccat
    21361 cgaccgcgcc atggaggccg gcattccggt gctggcgtcg aatgtcgatc atcccgacaa
    21421 gacgcaccgg ctctccttca ccggccagga cctcgagcag gcgggctacg acctcgccgc
    21481 cggcctggcg ccgcagttcc ccgcggaggg gccgatccat gtgctgatcg gcatctccgg
    21541 gccgggtcag gtctgggccg agagccgggg tgccggcatc gcgcgcttca tggacgacta
    21601 catcgccgac aatcccggcc gcgagatcac ttacgagaag atcgacagcg gcctcgatct
    21661 cgccatcacc ggccagcgtg tcgccgccta tgtgcagacc acgccgacca cggcctattt
    21721 cgacgtcggc tactgggcgg ccggtgccgc ggtcagcttg cgcgatctcg gcaaggaacc
    21781 cggcgaggtg ctgctcgcct gcttcgatct cgtgccggtg gtgctggacg agatgaagac
    21841 cggctacatc cagctcacca tcgaccagca gcccttcctc cagggctatg tgccgatcca
    21901 ccagctgcac atgatgaaca agtacaagct cggcgcctat gacgcgaaca ccggcaaggc
    21961 gctggtcaca cccgaccagg tggacgcgct gctcgagctc tcggcgcagg gcatccgctg
    22021 accgccattg tctgaaccat tcgctccggg ccacccgcgc ggtggcccgg aagccttttc
    22081 gggtgcctga tgcagtcgat cctgaagacc ctcctgtcgc ggccggagat cagcgcgctc
    22141 gtcatgctgg tggtggtcgt cgccggcttc agcttcatgg cgccgcagtt cgcctcctac
    22201 ggcaacacgc gcgtcctgct cttctcgctg ccggagctcg gcatcgtcgt gctcggcgtc
    22261 ggcatcctga tgatcgtcgg cgagttcgac ctctcggtcg gctcggtctt cgccctcgtg
    22321 ccgatgctga tggtcatcgc catggggcgc tggggcgtgc cgccgatcgt cgccatgctc
    22381 atgggcttcg ccctggcgct ggcggtcggc tacatcaacg gctggatcac gctgaccttc
    22441 aacatcccga gcttcgtcac gacgctcggc atgctgttca tggcgcgctc gctggcgacg
    22501 gtgctctcgg gcgggttccc gccgcccttt ccggccaacc tctcgaccga cctcttcgtc
    22561 gccgatctcg gcctcttccg gagctcgatg ctctggttcg ccggcttcgc gctgatcctg
    22621 ggcatcgtcc tgcacttctc caatctcggc aactggatct tcgcgaccgg cgggcagagc
    22681 caggccgccg ccgacatggg catcgacacg cgcaaggtga agctcttctg cttcatgctc
    22741 tgcagcttcc tcgcgggctt cgccgggatg atcaccacgc tccggctgcg ctcggccctg
    22801 ccggctctgg gcgaggggct ggaattgcag gcgatcgcgg cggcggtgat cggcggtgcc
    22861 gcgctcaccg gcggcatcgg ctcgctcgtc ggcatgctcg tcggcacgac gctgatccgg
    22921 gtgatcgaca acggcctcgt catggcgcgc atcgacgcca actggttccg cttcgccatc
    22981 ggtgcgctga ccatcgtcgc cgtcatcctc aacacctggg tccgggcgcg ggcccggcgg
    23041 atgaagtgag gaggggcgca tgacggcccg gctcgaggag catttccagg cgacggagac
    23101 cttcgtccgg ccgaccgccg aagcgctcgt gcgctgcgag gggctggaga agtggtacgg
    23161 cagcgtgcat gccctggccg aggtcgattt tcatgccgag ccgggcgaga tcgtcggcct
    23221 cgtcggcgac aacggcgccg gcaagtcgac actgatcaag atcctttcgg gggcgcatcc
    23281 ggccgatggc gggcgcatct tcttcgaggg ccgggaggtc cggctcgaca gcccgaaggc
    23341 ggcgatgggt ctcggcatcg agaccatcta ccagtacacg gcgatggtcc cggccatgtc
    23401 gatcgcgcgc aacatgttca tcggccgcga gcccttgacc cgcttcacca tcggcggcat
    23461 cggcctcatg gaccggcgca ccatggtcga gcacgcgacg cggtccttga ccaacatcga
    23521 cctcaccgag cgctcgcccg acaccacggt cgaggagctt tcgggcggcc agcgccaggc
    23581 ggtcgccatc gcccgggcga tgtacttcaa gtcgaaattg ctcatcctcg acgagccgac
    23641 caaccacctc tcggtcaagg agaccaacaa ggtgctggac tatgtcgcct cgctgaagga
    23701 gcagggcatc ggctcgatct tcatcagcca caacctgcac cacatctttc cgatctccga
    23761 ccgcatcgtc gccatggcgc gcgggcgaaa gatcgcggac atcaagaaga gcgacaccac
    23821 gatcgacgat ctgaccgacc tgatcgtctg agcagaaggc gggggagacc ggggcgatgg
    23881 agcacaaggg caggacggcg ctggtgacgg gggccacggg cgggctcggc gtcgccgaga
    23941 gcaagggcct ggcgctggcc ggtgcgaacg tcctgatgct cgacgtgaag gggcgcgagg
    24001 cggccgaggc gctggccggc gagctgccgg agggcgccgg ggcggtgcgc ttcgtgggct
    24061 gcgacctcgc cgatccggag gcggcgcagg acctggtgag gctgctggac gccgaggtcg
    24121 gcggcatcga catcctggtc aacaatgccg cgatcaatcc gctcaagccc gtcgacggct
    24181 acgacctcgc cgagtggcgc aaggtgcagg acgtgaatgc cacctccgcg gttgcgctgg
    24241 cccaggcggt ggtgccgtcg atgaagaaga agggctgggg gcagatcatc cagatctgct
    24301 cggtgacgct gaacggcggc tggtccgatt tcacctccta tgtgtcgtcc aagggcacgc
    24361 tgctggccct cacgcgctcg ctggcgcgcg agctcggcca gttcggcatc cgggtcaacg
    24421 cggtgagccc aggcgccatc ccgacggcgc tcgagaagga ggtctgggcc gaccagctcg
    24481 agacctacgt gaagttcctc gtcgaccatc aggcattgaa atatcgcggc agccccgagg
    24541 acatcgccga ggcgatcctc ttcctcgtct cggacaaggc gcgcttcatc accggccaga
    24601 acctctcggt cgacggcggc tggtggatgc actgaccggt gccatgcccg aagccatcag
    24661 cgagccgcgg cgcagctatc cgcgccacgg ggtgcacggc cggctggtcg aggcgatcgg
    24721 ccggctgatc atcggcgggg gcctggcggc cggtgccgtg ctgccgcgcg aggtcgagct
    24781 gatggacgag ttcggcagct cgcgcgcggc ggtgcgcgag gcggtcaagg tgctggccgc
    24841 caaggggctg gtcgagacgc gccagcgccg gggcatgcgg atcagggcgc tggaggagtg
    24901 gaacctgctc gacccggacg tgctcgcctg gtgcgccgcc tcggggccgg cgcctttgct
    24961 gatggcgcag atggtcgagc tgcgctgcct gatcgagccc cgggccgcgc ggctggcggc
    25021 agggcggcgc acggccgcgg agctcgaagc cctcgaggcg gcgcacgcgc aaatggtcgc
    25081 caccttcgcg gatgccgtag cctacaacct cgccgatctc gcttttcatc gggcggtctt
    25141 ccgggcctgc ggcaatccct tcgtcgaccg gctgggggcc atcgtcgccg tggtcctcga
    25201 gggcggcttc cgcctgcgtg acggggggat ggtcccgatc gaggccggac tcgttgcgca
    25261 cggccgggtg ctggccgcga tccgggacgg cgaagcggca gcggccgagg cggcgatgct
    25321 ggcggtcatc gaggtggcgc ggcgtgagct gccgcccgtc gatggcgggc cgtgctaggg
    25381 cgctcgagcc agctcgccgg ccctgtctcc ctggcagctc ccggcgcctc ggggcaacct
    25441 gcggcccaga ggcccgtctt gaccccaggg gtgtcgcgac cgatgttcga ggcttgcatc
    25501 aagagggaga agttgggtga acgaggacgt cttcaacatg gagctgcgca agttcctgaa
    25561 gcatgtgggc gtgaccagcc agcgggagat cgagcaggcg gtgcgcgacg cgctggcggc
    25621 gggcaagctg aagggggacg agaggctgcg ggcgaggatg gtgctgaccg tcggcgggct
    25681 cgacctcgag cacgagatca cgagcgacat cgccttgaag tgacgggcgg ctttgcccgc
    25741 acgcttctgg ccctgatcac gcttctggcc ggagcagggc cggtggcggc ggccttcatc
    25801 ggccatggcg ggccggtccg agggctagcc gtgactgccg atgccgcgac gctggtcagc
    25861 gccagcttcg actactcggt gatcctctgg cggctcgccg acggggcggc gagcgaggtg
    25921 ctgcatggcc acgacgcggc ggtcaacgcg gtggccctgc tgccggacgg ctccggcttc
    25981 gtcacggccg gcgacgatgc ggcggtgctg gtctggcgct tcggcgagcg ggcgccggtg
    26041 cgccggctca tcggtcacac cggccgggtc gtggcgctcg atgtggcgcc ggacggccgg
    26101 cgggtcgcct cggcggggtg ggaccggacg gccagggtct gggacgtcga gacgggtgcc
    26161 gagctgctcc ggctcgaggg tgccgcgaat gtcgacgcgg tccggttcac gggcgacggt
    26221 gcgacgatcg tcacggccgg ggcggatggt gccgtcaggc tctggcgggc ggcggacggg
    26281 acggcgctgg ccgtgctggg cggcgggccg gtggggctga atgctctcgt gctgggccgg
    26341 gacgggctgg cttatgccgc gggtgctgat ggtgcggtcg cggtctggga cctaacgtcg
    26401 ggcgagcggc gcgccgccct ctcgaccggt gtcgaggccc cgctcttcgc cctcgccatc
    26461 agctccgacg gccggctcct ggcggctgcc ggcatggccg ggacgatcac gctcttcgac
    26521 ctggcatccg gcgagatcgc ggcggtgctg gcgggcgagc ggcagccgct ctggtccttg
    26581 gccttctcgc cggacggagc aacgctctat gccggcggca acgaccggac catccgccag
    26641 ttcagcgtgg cggagggtgt cgagatcgag gcgcccgccg ccctgccgcc catggaggtc
    26701 gcggccacca acggcgatgc gggagcgctg gcctggcggc gctgcgtcgc ctgccacacg
    26761 ctgacccccg atggcggcaa ccgggccggg ccgacgctgc acggcatctt cgggcgacgg
    26821 atcggcacgg ttgctggcta cccctactcg ccggccctcg cgggtggcga tctggtctgg
    26881 accgaggaga cggtcggccg gctcttcgcg ctcgggccgg acgtggtgac gcccggcacc
    26941 aagatgccga tccagaggat cccggacgcg gcggagcggg cggcgttgat cgggtttctc
    27001 aagcgcgagg cgatgggcgg ggagtagggg gctcggaggg cacgtcagtt gcggtggccc
    27061 tcgtggcgag cggcgagaat ctcgtccgtg gagaagcgtg ccgcgtgcca tgaagcacga
    27121 cgaaggcgaa accgttcgag cgcagcggcg cggtcagtcg gatcagtgga ggcggcgggg
    27181 acgatgcggg cgatgggctt gccatggcac gtgacgacga aactctcacc ccgctccacg
    27241 gagctcaata tcgcggcgaa ttgggtcgtg acgtcggacg cccttactcg gcgcatgacc
    27301 tgctccaaaa tgtcagaatg gctgactgta acataatctc ctgagcgtcg aggagtcagg
    27361 tcgccgtcag ggcccctcac ccccgatagt cctccgcccc cttcgtcctc ggccgctccg
    27421 ggccggcata g
//
LOCUS       DAOTFI010000024        26745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2081_length_26745_cov_6.926002, whole genome shotgun sequence.
ACCESSION   DAOTFI010000024 DAOTFI010000000
VERSION     DAOTFI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 26745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26745
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2081_length_26745_cov_6.926002"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="P5558_03805"
     CDS             complement(<1..420)
                     /locus_tag="P5558_03805"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRY23468.1"
                     /translation="MAAPSFQELILRLQTYWAGHGCLILQPYDMEVGAGTFHPATTLR
                     ALGPEPWRAAYVQPSRRPTDGRYGLNPNRLQHYYQFQVILKPSPPDLQELYLQSLYTL
                     GIDPALHDLRFVEDDWESPTLGAWGLGWEVWCDGMEVS"
     gene            complement(505..1230)
                     /locus_tag="P5558_03810"
     CDS             complement(505..1230)
                     /locus_tag="P5558_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23469.1"
                     /translation="MTGSPIRAPGTATDRLRSLLADLVVHTSRSVFRRTPWNFDRANG
                     VETEAAVGEVALGIDVENPDGRCKYEASSLKHVRFAFGRIPEPLANWSFVDIGSGKGR
                     IVLIALGFPFRRVAGVEYAAELHAIAAANLDRYRGPRRAAAVDLVHGDALCVQLPPGD
                     TVVFFYNSFGGAMLEGYLDHLEGTVQGAPRRLLFIYSNPTERFRLDRRAAFRPVFEGA
                     SGYDLTWWGNRRLVVYEVGAAQP"
     gene            complement(1339..1599)
                     /locus_tag="P5558_03815"
     CDS             complement(1339..1599)
                     /locus_tag="P5558_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23470.1"
                     /translation="MLGLSLSKLLFTALLIVAVWKGWKLMTAIRDKISDPADYAGVKR
                     PEKQARPKPGPAETLYECPHCGTYVPNGTICRSKEECVLKKA"
     gene            complement(1719..2558)
                     /locus_tag="P5558_03820"
     CDS             complement(1719..2558)
                     /locus_tag="P5558_03820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRY23471.1"
                     /translation="MPWYWPDRLLRRSTPIPVVRLSGVIGAVPFRGGLTLRAIESALA
                     SAFAMKRAPVVALAINSPGGSPVQSSLIARRIRDLATEHRKPVVAFVEDIAASGGYWL
                     ALAADEILVDRSSILGSIGVISAGFGLEGALQRLGIERRVHATGPRKALLDPFRPEQP
                     GDLEVLAELHQDILASFKAQLEARRGPKLRLAPEELYSGRVWSGDRAIEHGLADGLGE
                     LRSTLRSRHGKQARLVVVNRQRGWLQRRLGGAGGADDRLLALLPQLLGQLEADAHWRR
                     FGL"
     gene            complement(2593..3366)
                     /locus_tag="P5558_03825"
     CDS             complement(2593..3366)
                     /locus_tag="P5558_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY23472.1"
                     /translation="MTGPAASPLAAACTTDLLLGGRLTIRQPARGYRAAIDPVLLAAA
                     VPANAGQTVLEAGTGVGTAALCLAARVEGIRIVGLEIDAVTAEMARRNVALNGREAAI
                     TIASGDILAPPADLRGRSFELVMSNPPFHPAATPPSPDAAKALATREGSSVADWLGAC
                     LRRLLPGGRLVLIQRADRLTELFAGLAGRAGEIEIVPLWPKAGRPAKRVIVRCRKGAK
                     GADRLAPGLVLHDGDGKYTAAAEAILRDGADLDSVLQQG"
     gene            complement(3363..3569)
                     /locus_tag="P5558_03830"
     CDS             complement(3363..3569)
                     /locus_tag="P5558_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRY23473.1"
                     /translation="MRSNDLVHLSWVDALMREAGIATLVLDEHTSSIEGSIGAIQRRV
                     LVAEDDLPRARRLLAEASGGSGER"
     gene            3736..4749
                     /locus_tag="P5558_03835"
     CDS             3736..4749
                     /locus_tag="P5558_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY23474.1"
                     /translation="MQQPSRQRPAEAPPLSLVKRLVADDLAAVNSLILERLQSEVTLI
                     PELARHLIAAGGKRVRPTLTLLAARLCGYEGDRHIALATSVEFIHTATLLHDDVVDES
                     DLRRGRSTANALWGNKAPVLVGDFLFSRAFQLMVADGNIRVLDILANASAVIAEGEVA
                     QLLTAGDVQTTEAAYYDVIRAKTAALFQAACRIGAVVADRPAVEEEALATFGDELGIA
                     FQLVDDALDYSAREAQLGKTIGDDFREGKASLPVLLAYAAGNPAERAFWERVIGEPAG
                     QREDDLAEAQLLLARHGTLDATLAQARASGQRARGCLEVFERSPWRDALEEIVDFSIA
                     RAY"
     gene            4805..4881
                     /locus_tag="P5558_03840"
     tRNA            4805..4881
                     /locus_tag="P5558_03840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4839..4841,aa:Pro,seq:cgg)
     gene            4957..5574
                     /locus_tag="P5558_03845"
     CDS             4957..5574
                     /locus_tag="P5558_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRY23475.1"
                     /translation="MRLELLYFANPMCSWCWGFAPAVQALAEAGHRITLAQGSLGADR
                     ARPMRPEDKASVRQHWEHVAERSGQPFDFGFFGRDGFVYDTEPACRAVEVVRRTFPAL
                     ALPAFARLQERFYALGHDVTDPSLLRAAAAEFGIAPEAFDRGFAAAETADRVAAEWQQ
                     TARLGVTGYPTLLAFAGGRPEVVTIGWRPPEEVLAAVDALAGTGA"
     gene            complement(5589..6218)
                     /locus_tag="P5558_03850"
     CDS             complement(5589..6218)
                     /locus_tag="P5558_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRY23476.1"
                     /translation="MRKRRLAHFAVAAMLLVATAMAGRPLQAADDGVFRLRGAGSWTC
                     SQFLEVVKNQDERGLYLAGGWIDGYVTALNDRVDGVFEMFPWQSTELVMELVHNHCER
                     VPEEMFFAAVARLVVVTKPTRLAKKSEIVEIPVPEGKPIQLYKPSLAKAQEQLKALGH
                     YTSTVDGAFGPGTAAAFRAFQAAAGLPESGLPDQITLWTLFAPLYEQAG"
     gene            complement(6288..6905)
                     /locus_tag="P5558_03855"
     CDS             complement(6288..6905)
                     /locus_tag="P5558_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRY23477.1"
                     /translation="MRRVLACLLVLLPALAGCGRLIPAHHQTVERSALRAGAYRLDPE
                     HSTILWKVAHLGYSTFVGRFDRYDATLDFDPEQPEDSVLEVSVETGSIDSHVPGFADT
                     LRGAAWLDAETYPEARFVSREIRITGDASGEVVGDFTLLGVTRPLTLDVVFNGGATNF
                     VTGRYTLGFAASAVLDRTAFGMTSLAPAIGGEVTLEIHAEFLAES"
     gene            complement(6920..7450)
                     /locus_tag="P5558_03860"
     CDS             complement(6920..7450)
                     /locus_tag="P5558_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRY23478.1"
                     /translation="MVDRRSLGRMRRARLVPLVVAALLAGCATAPDGVPSLPTAPAAP
                     TVAAGANGAAAAARHAIHPEIARATLPAPSAAAEVGEASWYGDAFAGRPTASGEPFDP
                     EALTAAHPELPFGTAVKVVNLDNGRHVEVVINDRGPFIADRIIDLSRAAARVLGFLRD
                     GITRVRVLPIGGADSA"
     gene            complement(7625..9343)
                     /gene="araD"
                     /locus_tag="P5558_03865"
     CDS             complement(7625..9343)
                     /gene="araD"
                     /locus_tag="P5558_03865"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HRY23479.1"
                     /translation="MTRKKPEELRSHRWFGVPDLRAFGHRSRIKQIGFDAVDYTGKPV
                     IGIINTWSDVNSCHTHFKQRVEDVKRGVWQAGGFPLELPAISLAEPIVKPTTMLYRNF
                     LAMETEELLRSHPIDGAVLMGGCDKTTPGLIMGAISMDVPAIFLPAGPMLRGNWHGKI
                     LGSGSDSWKYWAELKAGNITEEAWDEIENGIARSVGTCMTMGTASTMTAIAETMGWSL
                     PGASSIPAPDSNHTRMAAACGRRIVEMVWDDLKPSDFMTAQSVDNAIRCHLAMGGSTN
                     AMVHVVAMARRAGIPLTVERFDELAREIPVLANVRPSGKYLMEDFYYAGGIRALLMRL
                     EGELSLDCLTVNGRTLGENIAGAEVYLDDVIHTMESPVSKEAAAILKGNLAPDGCVMK
                     PSAAEPRLLKHRGPAVAFEDYNDMAARIDTAELDVTLDSILVLKNAGPIGGPGMPEWG
                     MLPIPKKLLKEGVRDMLRISDARMSGTSYGACILHVSPEAAIGGTLALVESGDMIEVD
                     VEARTIHLDVPEAELQKRREAWQPKPVVGRGYERMFAAHIQQAHDGCDFDFLTGTERL
                     PEPEIH"
     gene            9524..10456
                     /locus_tag="P5558_03870"
     CDS             9524..10456
                     /locus_tag="P5558_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY23480.1"
                     /translation="MSERKNEYEIIERPFGNLIGGNMRLRQLWTGAAWAEGPVYFRDG
                     DFLLFSDIPNNRIMRFVPDQTGLEGTTSVYRQPSFNTNGHTRDREGRLISCSHGARCV
                     FRTELDGTVTTLVDSFEGKRLNSPNDVVVKSDGTIWFTDPSYGILSDLEGHQGEPEYG
                     GCHVFRLDPGNGSLTAVCTDFVKPNGLAFSPDEKLLYIADTGASHDPDGPRHIRKFEV
                     RGDNTLAGGEVFATCDAGLFDGFRCDTDGRIWSSAADGVHCFDPAGTLIGKIHVPEVV
                     ANVCFGGAKRNRLYICGTTSLYAVYTHVNGAQTP"
     gene            10488..11276
                     /locus_tag="P5558_03875"
     CDS             10488..11276
                     /locus_tag="P5558_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRY23481.1"
                     /translation="MSYYLSKLAWLVLMPSNFLGLLLLLGVVLRRWWLVRVVVALLVP
                     VVVVPVGLWLHLPLEDRFARPAVMPERVDGIIVLGGAQEQQVTTGRGVLALERNGERL
                     IEGVALARRYPEARLVFSGRTGVPGGVGGSEQIVNELFIDLMGFDQSRVTYEDRSRNT
                     WENALFTREAVDPRPDEVWLLVTSAAHMPRSMGIFQRLGWEVVPWPVDYRTQGGLRWP
                     LNFSAAARLDELDMAAREWLGLLAYRLMGRTSALLPAPLPAPAS"
     gene            11343..12356
                     /locus_tag="P5558_03880"
     CDS             11343..12356
                     /locus_tag="P5558_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY23482.1"
                     /translation="MTTNRRTALLGAATIALAATIATPAAAQEQSFILTTATTGGTYY
                     PVGVALATLVKVKLQASDGIDMSAISSAGSGENIKLMREGQAQFGILQGLFGRYAAKG
                     SGPLEGEGKQEWLRSISMLWQNVEHFITTEDLAPTGTIDDLKGFAGKPFSIGAKNSGT
                     EGSGREILNGLGIDPESMDLVYQGYGPTAEGLQNGTIVGANMPGGVPVSAVTQAFAAR
                     DDLVILDFTAEQIERANGGDELWTAYVIPAESYPGQAEDASTIAQPNFLGVHADIDEE
                     AVYKVTKTMFENLGFLQNIHPATKAMSLEAALAGLPAPLHPGAVRYYQEAGVEIPARL
                     MPN"
     gene            12419..14539
                     /locus_tag="P5558_03885"
     CDS             12419..14539
                     /locus_tag="P5558_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRY23483.1"
                     /translation="MTEKLDEAGELTTPTRAGTGPLYARAVLALSVLISLFHIYANSI
                     GRLDDLLLTSMHWGGFALLCTLLYPLRPGGGRLVLGIDIALGLLAVAAAVYVNWAFDA
                     IYDRGVRFIWSDWVMACLAIALALEMARRTAGLFIPCLAVVALVYLAGGGAWVPGMFK
                     FPGLTLETTLFRAYVELVGMFGPIARISATFVFMFVLFGAFLVRSGAGAFVIDLARAV
                     AGRVVGGPGFVAVLGSALTGTVTGSAVANTVSTGVITIPLMKKAGFPAKFAAGVEASA
                     STGGQLMPPIMGAGAFVMATYTQISYLTIVAVSVLPAILYFFSVAVWVRIEAKKHDIT
                     GVDEDSPGALDILRRGGISFLIPMAVLIVMLLQGFTPTYAAAFAILAVIGSSWLTKTP
                     MGLLAIVDALALGARNMVMTAMLLVSVGIIVMVVETTGIGNTLSLMLAEWAGGNLMIA
                     LVLVALASLVLGMGLPVTAAYIVLATLSAPALYQMMNDAAIVDMIAQGTLPQAAQAVF
                     MLADPDAMTKLQGPLPAAEAAALYALVPSDFSRTLYQQALDPALLTTTLLSAHMIIFW
                     LSQDSNVTPPVCLTAFAAAAIAKTPPMATGLTAWRIAKGLYIVPILFAYTPYLNGDWL
                     TGLEITFFAAFGIYALAVGFEGHGEHPIPHWLRPVIFAIGVFLIWPTGRLSQIAAVVA
                     LVAVMLLAAKLAPKREYASPVETA"
     gene            complement(14550..14756)
                     /locus_tag="P5558_03890"
     CDS             complement(14550..14756)
                     /locus_tag="P5558_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23484.1"
                     /translation="MDRKVTTSAQRTAASRKRPAATRRPLHYRLLHSTGEHFIEDRDQ
                     AIDRLASLPGAKLFVLRDAAPVAG"
     gene            15498..15980
                     /gene="dps"
                     /locus_tag="P5558_03895"
                     /gene_synonym="pexB"
     CDS             15498..15980
                     /gene="dps"
                     /locus_tag="P5558_03895"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HRY23485.1"
                     /translation="MPTAFVENLDRSVRTEMIEELNARLVEALDLVLAVKHAHWNIKG
                     PSFIGIHLLLDEVAQRMRGHADTMAERCVILGGQAKGTVQVVAEETTLEAYPPEITRQ
                     EEHVTALKERLMAFGKAVRESISKADEAGDDDTADLFTAVSRAVDKDAWFIGAHGERS
                     "
     gene            complement(15987..16595)
                     /locus_tag="P5558_03900"
     CDS             complement(15987..16595)
                     /locus_tag="P5558_03900"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRY23486.1"
                     /translation="MPGPWIAAGLLLALAVAAPAGAQDGATGQTLEQLEGLEEEAAGG
                     SGAPAAEPLTKTEAAAVDPEGAAPLEDPLTCLARTIYWEAKGEDVAAMEAVANVVMNR
                     LAAEPFPDTICGVVTEGKEGGPCQFAWWCDGSPDDVEEPDRYAITTDVARRALNQELP
                     DRTDGALFFHGINDTPDWAADYVETARVGDHVFYRPANEGGG"
     gene            complement(16595..17125)
                     /locus_tag="P5558_03905"
     CDS             complement(16595..17125)
                     /locus_tag="P5558_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY23487.1"
                     /translation="MAEVETLLLEPGHDVPNSPLPVLLYRAACEAGPGLGDRLERLFR
                     ANGWGGTWQNGIFPYQHFHDDAHEVLGIARGEAVVQLGGAGGPEVSVRAGDVVVLPAG
                     TGHKRLSSSADLLVVGGYPPGQEGPSVLRPAGESMTSLVGRCAAVALPETDPVEGADG
                     PLTRLWRGGDEPAGGA"
     gene            complement(17131..17958)
                     /locus_tag="P5558_03910"
     CDS             complement(17131..17958)
                     /locus_tag="P5558_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY23488.1"
                     /translation="MTGPKLQTPPGATDTHMHFYGPYDRWPLPPTSWNSPPEATLAQY
                     REVQKRLGLERVVVVQAAGYGYDNTVTMDNVGVIGETARAIVVVPKGTAEAELDRLNR
                     AGARGLRVFMLKGGVYDWADLPELAAMVKPFGWHLQVQLDGRLLPEHVDALLGLGVQL
                     VIDHTGKYLEPVDVDHPAFACLRRLVGAGAYVKLSAPYETSKVGPPTYDDVGRLARVL
                     VEDAPERMLWASNWPHPGHAPKDDAMLLDTLLDWAPDEADRRRILVDNPARLYGFQE"
     gene            18012..18686
                     /locus_tag="P5558_03915"
     CDS             18012..18686
                     /locus_tag="P5558_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HRY23489.1"
                     /translation="MVSIDERLADWPSDLATARAMQEMVRHLVSERAPAGFVPRLVAG
                     VDVHFSRDGRTAFAAAAVLDAGSHELVESAQLSLPVGFPYRSGFLSFREVPAALAVLG
                     LLAARYDLVLVDGQGRAHPRRCGFASHLGVLLDRPTIGVAKSRLIGSFVEPAPERGAA
                     SPLHDRAEIIGAVVRSKAGCRPLFVSVGHLIDLDGAVAAVLALTGRYRLPEPIRQADR
                     LSRAHG"
     gene            complement(18619..18972)
                     /locus_tag="P5558_03920"
     CDS             complement(18619..18972)
                     /locus_tag="P5558_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23490.1"
                     /translation="MGLLKEELLAATGADRELDRRLAAELGAPPAAYTASIDAALDLI
                     RHALPGWGWHLGWHADGIRPYASLHDAARTVHVEAAGPTVPVALLRAMGEALIHVPGT
                     GDPPAGSAPAGDSAR"
     gene            complement(18960..19952)
                     /gene="dhaK"
                     /locus_tag="P5558_03925"
     CDS             complement(18960..19952)
                     /gene="dhaK"
                     /locus_tag="P5558_03925"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HRY23491.1"
                     /translation="MKKLINDVDAILGESLAGFARAHGDLVSLSQAPLFVRRRAVTPA
                     GQVALISGGGSGHEPLHAGFVGRGMLHAACPGQVFTSPTPDQMLAAAEAVDSGAGVLF
                     IVKNYEGDVMNFEMAAEMAPCATARVLTNDDVAVENSSYTTGRRGVAGTVVVEKMLGA
                     AAETGMDLATLQALGERVNGATRSMGCALTSCTVPAAGGPTFEIGEAEMEMGVGIHGE
                     PGRRRVALASASAIVEEMMGAILADLGAAKGEDVLLLVNGFGATPLMELYLVYDEAAR
                     RVEAAGLTVARSLVGNWCTSLDMAGCSLTVTRLDAETTGLWDAPVHTPALRWGC"
     gene            complement(20041..20673)
                     /gene="dhaL"
                     /locus_tag="P5558_03930"
     CDS             complement(20041..20673)
                     /gene="dhaL"
                     /locus_tag="P5558_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HRY23492.1"
                     /translation="MSNTLVLRLIEGMAGTIESHADQLTRLDQAIGDGDHGINMARGF
                     KELAAQKAAIAALPLGEALKKAGMTLVMKVGGASGPLYGTLFIDMGKATPEAAPTLAD
                     LSVMLDAGIRGVKARGKAEVGMKTMLDVLCPVADTLRSLVDQNETDGLDERIIAAAGH
                     ALHRTTDMVAKRGRASFLGERSKGHLDPGACSSALLIGAVVGVLAPSSAR"
     gene            complement(20759..21928)
                     /locus_tag="P5558_03935"
     CDS             complement(20759..21928)
                     /locus_tag="P5558_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRY23493.1"
                     /translation="MSSRAMGVGTSGVTIDGRIVGAALLLLVVLVWQAGQAAGPNMAA
                     LAVVGGLLGLVLHHASFGFTAAWRRFVTTGEGHGLRAQMLMLALAVCLFFPALAAGEL
                     FGRPVGGFVFPVGLGVVAGAFVFGIGMQLGGGCGSGTLFTVGGGSTRMVLTLFFFVAG
                     SVVAIGHLPFWHGLPGIGGVSAITMLGLWPALLLHLALFALIVALTLQVERRRGVSSR
                     PRTAGDWLRGPWPILAGAAALAVLNFLTLWLAGRPWGITSAFALWGAKGLDALGVEIA
                     AWHNWGGMAGAIERSVLFDVTSVMNFGIVLGAMLAAGLGGRFRPTLRLGAGDIGSAVL
                     GGLLMGYGARLAFGCNIGAFFSGIASGSLHGWVWLVAAFAGSMIGVRLRPLFGQP"
     gene            complement(21993..23258)
                     /locus_tag="P5558_03940"
     CDS             complement(21993..23258)
                     /locus_tag="P5558_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRY23494.1"
                     /translation="MITLYIVAILILLLLSAAFSGSETAVTATGRARATALAQGGNRR
                     AGTLLSLLNDRERLISTLLLGNNIVNIMAAALATDVLLRLFGNAGVVYATAVMTLLIV
                     IFSEVVPKTVALRHHDTLALAIAGPLRVLVVLFRPFTTAIGWVVRLVLRGLRIPTHGG
                     DVISASQQLRGLIDAFEAGGELAKHAGDMLDGVLDLEHVTVDEVMTHRRRVTALDASM
                     TIEAAVARLRDEPYSRFPVYRGSSDHIVGILHLRDLLTAVADREAGASRTVGEITRDP
                     WFVPETTPLRKQLEQFRKKKQHMAFVVDEYGDLQGLVTLQDVLEAIVGEVSEADDREF
                     DEIERLPDGSLQTSGTTQIRRLNRLLEWDLPEEDAVTLAGLVIDVAERIPLEGETLAI
                     GRYRVTVKKRERNRLSVLTISRAEPDESD"
     gene            complement(23365..25455)
                     /locus_tag="P5558_03945"
     CDS             complement(23365..25455)
                     /locus_tag="P5558_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23495.1"
                     /translation="MADATPILECRNLCISYFTRAGEIPAVVDFDLIVMPGEAVGIVG
                     ESGCGKSTVALAIMQYLGGNGRIVSGEIRFMGQDLTTMSQEELRQLRGSKIAMVYQEP
                     MASLNPSMTIARQLMEVPLYHEAGCSEADARQRALEMLADVRLPDPARIMDAYPHQIS
                     GGQQQRVVIAMALLSNPSLLLLDEPTTALDVTVEAGIVQLVREISERYGTSMIYISHN
                     LGLILETCDRITVMYSGEAVEEGSVEQVFDRMRHPYTKGLFNSIPLPGADKHARPLMP
                     IPGQLPLPHERPPGCNFGPRCDHFRAGVCDAGPIAMATVPAHQRHYTRCARFSEIDWQ
                     ARPEVVEAAASANLGEVVLRVENMKKYYELRDPSLMALISGRSVRTVKANEKLDFEAR
                     EAETVAIVGESGCGKSTFAKVMMGLETATEGKLILDGKDLAQTPVERRRPQVVGHLQM
                     VFQNPFDTLNPSHTVGGQIARVIRKFGVESDQAKVEARVMELLDIVKLPRDFYKRRPR
                     QLSGGQKQRVGIARAFAGNPRVVVADEPVSALDVSVQAAVSQLLMDIQRENRTTLIFI
                     SHDLSLVRYLADRVVVMYLGTIVEQGTTDEVFAPPYHPYTEALLSAVPIADTSIRKKI
                     IVLHGDIPSAVDPPSGCPFQTRCPRKLGAICDTEEPPIREFSAGHRIACHIEPALLAE
                     MEPVITMPPEQAAE"
     gene            complement(25461..26471)
                     /locus_tag="P5558_03950"
     CDS             complement(25461..26471)
                     /locus_tag="P5558_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23496.1"
                     /translation="MATIAAPTSPAPSLLDRLRRPRVLLGLVALLWLPLVAYVMFGVG
                     RMVLIPAIPATLLLYALAVISWRESAVAVIGVTLVMFWLFIAVVVPFLPLLDPNQPVQ
                     PFVTPLTEARDMFFWLGTDFRGRDMLSRTLWGCQRVLVWGVTATAVAYVIGTALGLVA
                     GYLAGWWDEVISFIGNVILSFPVMVLFIVILTYFGPSGFNIIVAVTFASAPAIMRIVR
                     GLTLDIKTRDYVFAAQTRGERPLWIMVVELLPNARGPIIVDACLRLGYTTVAITTLTF
                     LGLGLQPPDPDWGLMIKEAAAAAMLFKFSYMLLVPALSVSSLILGFNLMADGLREMSL
                     RD"
     gene            complement(26484..>26745)
                     /locus_tag="P5558_03955"
     CDS             complement(26484..>26745)
                     /locus_tag="P5558_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY23497.1"
                     /translation="RHALRNALIAPFTVIVLQLTWLLSGVVVVEVFFEYSGFGKLLLE
                     AALFGDIYVVQAATFVAVIVAVLSQIVSDVGYTLLNPRIRFG"
BASE COUNT         4150 a         9582 c         9046 g         3967 t
ORIGIN      
        1 actcacctcc atcccgtcgc accagacctc ccaacccagc ccccaggcac ccagcgtcgg
       61 actctcccag tcgtcctcga cgaagcgcag gtcgtgcagc gccgggtcga tgccgagcgt
      121 gtagaggctc tgcagataga gctcctgcag gtccggcggc gacggcttga ggatcacctg
      181 gaactggtaa tagtgctgca gccgattggg gttgagcccg tagcggccat cggtcggccg
      241 ccgcgacggc tgcacatagg ccgcccgcca cggctccggg ccgagggcgc gcagcgtggt
      301 cgcgggatgg aacgtcccgg cccccacctc catgtcgtag ggctgcagga tcaggcagcc
      361 atggcccgcc caataggtct ggagccggag gatcagctcc tggaagctcg gcgcggccac
      421 gtcgcttatc aacccgatgg tttgctgcgg tgcagaacct aggctagggc gaccggcccg
      481 gtcaagcgat cggccgggcg ccgctcaagg ctgggccgcg ccgacctcgt agacgacgag
      541 gcggcggttg ccccaccagg tgagatcata gccggaagca ccctcgaaaa ccgggcgaaa
      601 agcggcccgc cggtcgaggc ggaaccgctc ggtcgggttg ctgtagatga agagcaaacg
      661 gcgcggcgcg ccctgcaccg tgccctcgag atggtcgaga tagccttcga gcatcgcccc
      721 gccgaaggag ttgtagaaaa agacgacggt gtcgccgggc ggcagctgta cacacagcgc
      781 gtcgccatgg acgagatcga cggcagcggc ccggcgtggg ccccggtagc ggtcgagatt
      841 ggctgcggcg atggcgtgca gctccgccgc gtactcgacg ccagccaccc gccggaacgg
      901 aaaacccagc gcgatcagca cgatccggcc cttgcccgag ccgatatcga cgaatgacca
      961 gttggcgagc ggctcgggga ttcgcccgaa ggcgaagcgg acatgcttga gcgagctggc
     1021 ctcgtatttg caacggccat cagggttttc gacatcgatg ccgagcgcga cctcccccac
     1081 ggcagcttcg gtctcgacgc cgttggcgcg gtcgaagttc cagggcgtgc gccgaaaaac
     1141 cgaccggctg gtgtgaacca ccagatcggc gagcagggag cgcaggcgat cggtggccgt
     1201 gcctggcgca cggatcggcg aacccgtcat gagctggcgc ggctccctcg ctctggtcgt
     1261 gcgggagagc tagggcagat atggccaatc gatcgggggt tcggaccgat agcgtacctg
     1321 gagtcgcaaa cggtcgaatc acgccttctt gaggacgcac tcctccttcg accggcagat
     1381 cgtgccgttc ggcacatagg tgccgcaatg cgggcattcg tagagcgtct cggccgggcc
     1441 gggcttcggc cgcgcctgct tctcgggccg cttgacgcca gcatagtccg ccgggtcgct
     1501 gatcttgtcg cggatcgccg tcatgagctt ccagcccttc cagacggcga cgatcaggag
     1561 ggcggtgaag agcagcttcg agagcgacag accgagcatg gaaagcggcg ccataggaag
     1621 aggggggtga ggcggggcag gatcgaaaca ggcagcagtc tagcaggtgg cggcgggcgc
     1681 tgtctcccgc aagccggccg ggcggcaagg ggcggggatc agagcccgaa ccgccgccag
     1741 tgcgcgtccg cctcgagctg gcccagaagc tgcggcagca gggccaggag ccggtcatcg
     1801 gcgcccccgg ccccgccgag gcgccgctgc agccagcccc gctgccggtt gacgaccacc
     1861 aggcgggcct gcttgccgtg gcgggaacgc agggtcgagc gcagctcgcc gagcccgtcg
     1921 gcgaggccgt gctcgatcgc ccggtcgccg ctccagaccc gacccgagta gagctcctcg
     1981 ggcgccaggc ggagcttcgg gccccgccgc gcctcgagct gcgccttgaa gctcgcgagg
     2041 atgtcctggt gcagctccgc cagcacctcg agatcgccgg gctgctccgg ccggaaaggg
     2101 tcgagcaacg ccttcctggg gccggtcgca tggacgcgcc gctcgatgcc gagccgctgc
     2161 agcgccccct cgagcccgaa gccggccgag atgacgccga tcgagcccag gatcgacgag
     2221 cggtcgacga ggatctcgtc ggcggcgagc gccagccagt agccgcccga ggcggcgatg
     2281 tcctcgacga aggcgaccac cggctttctg tgctccgtgg cgaggtcgcg gatgcgccgg
     2341 gcgatcagcg aggactggac cggcgagccg cccgggctgt tgatggcgag cgccaccacc
     2401 ggggcgcgct tcatggcgaa ggcgctggcg agggcggatt cgatcgcccg cagcgtcaac
     2461 ccgccgcgaa agggcacggc accgatcacg ccgctcaggc gaacgacggg gatcggcgtg
     2521 ctgcggcgaa ggagccggtc gggccagtac cagggcaagg tcgaaatccc tctcgttggc
     2581 gcaggtcggg cctcagcctt gctgcaggac gctgtcgagg tcggcgccat cccgcaggat
     2641 cgcctcggcc gcagccgtgt acttgccatc gccgtcatgc aggacaagcc ccggggcgag
     2701 ccggtccgct cccttggccc ccttgcgaca gcgaacaatg acccgcttgg ccggccggcc
     2761 ggccttcggc cagagcggca cgatctcgat ctcgcccgcc cgcccggcga gcccggcaaa
     2821 gagctccgtc agccggtcgg cgcgctggat cagcaccagc cgcccgccgg gcaagagccg
     2881 ccgcaggcag gcgccgagcc agtcggccac gctcgagccc tcgcgcgtcg ccagcgcctt
     2941 ggcggcatcg ggcgatggcg gcgtcgcggc cgggtgaaag ggcgggttgc tcatgaccag
     3001 ctcgaaggat cgaccgcgga gatcggcggg cggcgccagg atatcccccg acgcaatcgt
     3061 gatcgccgcc tcgcggccat tcagggcgac attgcggcgc gccatttcgg ccgtgaccgc
     3121 gtcgatctcg agccccacga tgcggatgcc ctcgaccctg gcggcgaggc acagggccgc
     3181 cgtgccgacc ccggtgccag cctcgagcac ggtctggccc gcgttggccg ggacggcggc
     3241 ggcgagcagc acgggatcga tggcggcgcg atagccccgg gccggctggc ggatcgtcaa
     3301 ccggccaccc agcagaagat cggtggtgca ggcagcagcg aggggcgacg cagccgggcc
     3361 tgtcaccgct cccctgaccc gcccgacgcc tcggcgagca ggcgccgggc gcgcggcagg
     3421 tcatcctccg ccaccagcac ccgacgctgg atggcgccga tgctgccctc gatgctgctc
     3481 gtgtgctcgt cgagcacgag cgtggcgatc cccgcctcgc gcatcagcgc gtcgacccac
     3541 gagaggtgga cgaggtcgtt gctgcgcacc aattctacca agatgcgacc atccgtcgaa
     3601 gccagctacg caagtgtctg gtttgccaga gttgaagcgg gcgtggtagc tagcgccgcg
     3661 ggcgttggca agcggtccat ccggaaggcc gcaggggcgc cctgttgaag tctggagtac
     3721 gccttggtga ccgccttgca acagccctcc cgccagcggc cggcggaagc gccgcctttg
     3781 tcgctggtca agcggctggt ggcggacgac ctcgcggcgg tcaacagcct catcctcgag
     3841 cggctgcaga gcgaggtcac cctcatcccc gagctcgccc gccacctgat cgcggccggc
     3901 ggcaagcgcg tccgcccgac gctgaccctg ctcgcggccc ggctctgcgg ctacgagggc
     3961 gaccgccaca tcgctctcgc cacctcggtc gagttcatcc acacggccac gctgctgcac
     4021 gacgacgtgg tcgacgagag cgacctccgg cgtggccgct cgaccgccaa cgcgctctgg
     4081 ggcaacaagg cgccggtcct ggtcggcgac tttctcttca gccgcgcctt ccagctcatg
     4141 gtcgccgacg gcaacatccg cgtgctcgac atcctcgcca acgcctcggc ggtcatcgcc
     4201 gagggcgagg tcgcccagct cctgaccgcc ggcgacgtgc agaccaccga ggccgcctat
     4261 tacgacgtca tccgcgccaa gaccgccgcg ctcttccagg ccgcctgccg catcggcgcc
     4321 gtcgtcgccg accgcccggc cgtggaggag gaggcgctcg ccaccttcgg cgacgagctc
     4381 ggcatcgcct tccagctcgt cgacgatgcg ctcgactact ccgcccgcga ggcgcagctc
     4441 ggcaagacca tcggcgacga tttccgcgag ggcaaggcga gcctgccggt gctcctcgcc
     4501 tacgcggcag gcaacccggc cgagcgcgcc ttctgggaac gggtgatcgg cgagcctgcc
     4561 gggcagcggg aagacgacct cgccgaggcg cagcttctcc tcgcccgcca cggcacgctc
     4621 gacgccaccc tggcgcaggc gcgagcctcc ggccagcggg caaggggctg cctcgaggtg
     4681 ttcgagcgct cgccctggcg ggacgcgctc gaggagatcg tcgatttctc catcgcccgc
     4741 gcctattgag gcatcagggt ggcttgcacc gccagatggt ttcgcctatc tctcgccgcg
     4801 gcctcggagt gtagctcagc ctggtagagc accgtcttcg ggaggcgggg gccgcaggtt
     4861 caaatcctgc cactccgacc accgagcggc gccggttccg gcgccgtttg tgcgttcggc
     4921 tcctgcgcgc ctccactcgt cgagggagaa gccgctttgc gcctcgagct gctctatttc
     4981 gccaatccca tgtgctcctg gtgctggggc ttcgcgccag ccgtgcaggc gctcgccgag
     5041 gccggccacc ggatcaccct cgcccagggc agcctcggcg cggaccgtgc cagacccatg
     5101 cggcccgagg acaaggcctc tgtccgccag cactgggagc atgtcgccga gcggagcggc
     5161 cagcccttcg atttcggctt cttcgggcgc gacggcttcg tctacgacac cgagcccgcc
     5221 tgccgggccg tcgaggtcgt gcgccggacc ttcccggccc tggccctgcc agccttcgcc
     5281 cggctgcagg agcgcttcta cgcgctcggc cacgacgtga ccgacccgtc gctgctccgc
     5341 gccgcagccg ccgagttcgg catcgcgccg gaagccttcg atcgaggatt cgcggcggcg
     5401 gagactgccg accgggtcgc cgccgagtgg cagcagacag cccggctcgg cgtcaccggc
     5461 tacccgacgc tcctcgcctt cgccggcggc cggccggagg tcgtgaccat cggctggcgg
     5521 ccgccggagg aagtgctcgc tgccgtcgat gccctggcgg gcaccggcgc ctgagacggc
     5581 ccgccggctc agccggcctg ctcgtagagc ggcgcaaaca gcgtccagag cgtgatctgg
     5641 tccggcaggc cgctctccgg caggcccgct gcggcctgaa aggcacgaaa ggcggcggcg
     5701 gtgcccggac cgaacgcccc gtccaccgtc gacgtatagt ggccgagcgc cttgagctgc
     5761 tcctgcgcct tcgccagcga tggcttgtag agctggatgg gcttgccctc gggaaccgga
     5821 atctcgacga tctcgctctt cttcgccagg cgcgtgggct tggtcaccac caccaggcgc
     5881 gcgacggccg caaagaacat ctcttccggg acgcgctcgc agtggttgtg caccagctcc
     5941 atgacgagct cggtgctctg ccaggggaac atctcgaaga cgccgtcgac ccggtcgttc
     6001 agtgccgtga catagccgtc gatccagccg ccggccaggt acaggccgcg ctcgtcctgg
     6061 ttcttgacga cctcgagaaa ctgggagcag gtccaggagc cggcaccccg cagccggaac
     6121 acgccgtcgt ccgccgcctg cagggggcgg ccggccatgg cggttgcgac cagcaacatc
     6181 gcagccacgg caaaatgcgc caggcgtctc tttcgcatgc agggctctcc ggttatcggg
     6241 cgatccagtc gaacggccac aaccatatag caggccccga cgccgtctca actctccgcc
     6301 agaaactccg catggatctc gagcgtcacc tcgccgccga tcgccggtgc cagactggtc
     6361 atgccgaacg cggtgcggtc gagcacggcc gacgcggcga agccgagcgt gtagcggccg
     6421 gtgacgaagt tggtggcccc gccattgaag acgacatcga gcgtcaacgg ccgggtgacg
     6481 cccagcagcg tgaagtcacc gaccacctcg ccgctcgcgt caccggtgat cctgatctcg
     6541 cggctgacga agcgggcctc gggataggtc tcggcgtcga gccaggcggc gccgcgcagc
     6601 gtgtcggcga agccgggcac atggctgtcg atgctgccgg tctcgacgct gacctcgagg
     6661 accgaatcct ccggctgctc cgggtcgaaa tcgagggtcg cgtcgtaccg gtcgaaccgg
     6721 ccgacgaagg tcgagtagcc gagatgggcg accttccaga ggatcgtcga atgctccggg
     6781 tcgagccgat aggccccggc ccggagcgcc gatcgctcga ccgtctggtg gtgcgccggg
     6841 atcagccggc cgcagccggc gagcgccggc agcaggacga ggaggcaggc aagaacgcgg
     6901 cgcatcggtc ccctctcctt caggcgctgt cggccccccc gatcggcagg acccgcacgc
     6961 gcgtgatccc gtccctgagg aagccgagca cgcgggccgc ggcccgcgac agatcgatga
     7021 tccggtcggc gatgaacggc ccccgatcgt tgatcaccac ctcgacatgg cgcccattgt
     7081 cgagattgac caccttcacg gcggtgccga agggcagctc cggatgggcc gccgtcagcg
     7141 cctcgggatc gaagggctcg ccgctcgccg tcggccgccc ggcgaacgca tcgccatacc
     7201 aggacgcctc gcccacctca gcagcggcgg atggcgccgg cagcgtggcc cgggcgatct
     7261 ccggatgaat agcgtgcctg gcggccgcgg ccgcgccatt tgcgccggcc gccacggtgg
     7321 gtgccgccgg cgccgtcggc agggatggaa cgccgtccgg tgcggtggcg cagccggcga
     7381 gcagggccgc gaccaccagc ggcacgagcc gggcgcggcg catgcgccca agggagcggc
     7441 gatcgaccat gggctcgtct cctccttatc gtcctcgacc ctgtcgggcc gagccggccc
     7501 ggccgacgcc gctgtggcaa tcctgccgcg gcaaccggcc gtcaccggaa ttttcttacc
     7561 ggcgtatgat ttagacgatc ggggttaacc tttggctaag ccgggccatt ttttcttcgc
     7621 aggctcagtg gatctccggc tccggcaggc gctcggtgcc ggtcaggaag tcgaagtcgc
     7681 acccgtcatg cgcctgctgg atgtgagccg cgaacatccg ctcgtaaccc cttccgacca
     7741 ccggcttcgg ctgccacgct tcacgtcttt tctgcagctc cgcctcgggc acgtcgaggt
     7801 ggatggtgcg ggcttccacg tcgacctcga tcatgtcgcc gctctcgacc agggccagcg
     7861 tgccgccgat cgcggcctcg ggcgagacgt gcagaatgca ggcgccatag gacgtcccgg
     7921 acatccgcgc atcggaaatg cgcagcatgt cgcgcacccc ctccttgagg agctttttcg
     7981 ggatcggcag catgccccat tccggcatgc ccggcccgcc gatcggcccg gcgttcttga
     8041 gcaccaggat cgaatccagg gtgacgtcga gctcggccgt gtcgatcctg gccgccatgt
     8101 cgttgtagtc ctcgaaggcg acggccggcc cccgatgctt gagcagccgc ggctcggcgg
     8161 ccgagggttt catgacgcag ccgtccggcg cgagattgcc cttgaggatg gccgccgcct
     8221 ccttgctcac cgggctctcc atggtgtgga tgacgtcgtc caggtagacc tcggcaccgg
     8281 cgatgttctc gcccagcgtt cggccgttga cggtcaggca gtcgaggctg agctccccct
     8341 cgagccgcat cagcagcgcc cggatgccgc cggcatagta gaagtcctcc atcaggtact
     8401 tgccggacgg ccggacattg gcgagcaccg ggatctcccg cgccagctcg tcgaaacgct
     8461 ccaccgtgag cgggatgcct gcccgccgcg ccatggcgac gacatggacc atggcgttgg
     8521 tcgacccgcc catggcgaga tggcagcgga tggcgttgtc gaccgattgc gcggtcatga
     8581 aatcggacgg cttgaggtcg tcccagacca tctcgacgat gcgccggccg caggccgccg
     8641 ccatgcgcgt gtggttgctg tcgggggcgg ggatcgagga ggcgcccggc aggctccagc
     8701 ccatggtctc ggcgatcgcc gtcatggtcg aggccgtgcc catggtcatg caggtgccca
     8761 cgctgcgggc gatgccgttc tcgatctcgt cccaggcctc ctcggtgatg ttgccggcct
     8821 tgagctcggc ccagtacttc caggaatcag aacccgagcc caggatcttg ccgtgccagt
     8881 tgccgcgcag catcggcccg gccggcagga agatcgccgg cacgtccatc gagatggccc
     8941 ccatgatcag gcccggcgtg gtcttgtcgc agccgcccat gagcaccgcc ccgtcgatcg
     9001 ggtgcgagcg caagagctcc tccgtctcca tggcgaggaa gttgcggtag agcatggtgg
     9061 tgggcttgac gatgggctcc gccagcgaga tcgccggcag ctccagggga aagccgcccg
     9121 cctgccagac gccgcgcttc acgtcctcga cccgctgctt gaaatgggtg tggcagctgt
     9181 tgacgtcgga ccaggtgttg atgatgccga tcaccggctt gccggtgtag tcgaccgcgt
     9241 cgaagccgat ctgcttgatc cgcgagcgat ggccgaaggc acgcaggtcc ggcaccccga
     9301 accagcgatg ggagcgcagc tcctcgggct tcttcctggt catctcgtcc ctccccttga
     9361 tcgtcctcgt ggcgcggccc cgttgcccgg cggggcatag ctagcatcgg ccggcgccct
     9421 gtgccatgcc gagtgccgcc gctctcggtc gatcacggcg ggccttcccg ccgaggcgcc
     9481 cagcggctaa agcggggccg gcaaccagaa cggggaggac gtcatgtcgg agcggaagaa
     9541 cgagtacgag atcatcgagc ggcccttcgg caacctgatc ggcggcaaca tgcggttgcg
     9601 ccagctctgg accggggccg cctgggccga ggggccggtc tatttccgcg acggcgactt
     9661 cctcctcttc tccgacatcc ccaacaaccg gatcatgcgc ttcgtccccg accaaaccgg
     9721 gctcgagggc acgacgagcg tctatcgcca gccgtccttc aacaccaacg gccacacccg
     9781 cgaccgcgag ggccggctga tctcctgcag ccacggggcg cgctgcgtct tccgcaccga
     9841 gctcgacggc acggtcacca ccctggtcga cagcttcgag ggcaagcgcc tgaactcgcc
     9901 caacgacgtg gtggtcaagt ccgacggcac gatctggttc accgacccgt cctacggcat
     9961 cctcagcgac ctcgagggcc accagggcga gcccgagtac ggcggctgcc acgtcttccg
    10021 cctcgacccc gggaacggca gcctcaccgc cgtctgcacc gacttcgtca agcccaacgg
    10081 gctcgccttc tcgccggacg agaagctgct ctacatcgcc gacaccggcg ccagccacga
    10141 cccggacggc cctcgccaca tccgcaagtt cgaggtgcgc ggcgacaaca cgctggccgg
    10201 cggcgaggtc ttcgccacct gcgatgccgg gctcttcgac ggctttcgct gcgacaccga
    10261 cgggcggatc tggtcgtccg ccgcggacgg tgtgcactgc ttcgatccgg ccggcacgct
    10321 gatcggcaag atccacgtgc ccgaggtcgt ggcgaacgtc tgcttcggcg gcgccaagcg
    10381 gaaccgcctc tacatctgcg gcaccacctc gctctacgcc gtctacaccc acgtcaacgg
    10441 cgcccagacg ccttgagcag gcctgccggg cgggcggacc ctggcggttg agctactacc
    10501 tctcgaagct cgcctggctc gtgctgatgc cgagcaactt cctcggcctc ctgctcctcc
    10561 tgggcgtggt gctgcgccgc tggtggctgg tccgggtcgt ggtcgccctc ctggtgccgg
    10621 tcgtcgtggt gccggtcggc ctctggctgc atctgccgct ggaggaccgc ttcgcccgtc
    10681 cggcggtgat gccggagcgg gtcgacggga tcatcgtcct cggcggcgcc caggagcagc
    10741 aggtcacgac cggccggggc gtgctggcgc tcgagcggaa cggcgagcgg ctgatcgagg
    10801 gggtcgcgct cgcccgccgc taccccgagg cgcgcctggt cttcagcggc cggaccggcg
    10861 tgccgggcgg ggtcggcggc agcgagcaga tcgtgaacga gctgttcatc gacctcatgg
    10921 gcttcgacca gagccgcgtc acctacgagg accggtcgcg caacacctgg gagaacgcgc
    10981 tcttcacccg ggaggccgtc gatccgcggc cggacgaggt ctggctcctg gtcacctccg
    11041 cggcgcacat gccgcgctcg atgggcatct tccagcggct gggctgggag gtcgtgccct
    11101 ggccggtcga ctaccggaca caaggcggcc tgcgctggcc cttgaatttc tcggcggcgg
    11161 cccggctcga cgagctcgac atggcggcgc gggaatggct cggcctcctc gcctaccggc
    11221 tgatgggccg gaccagcgcc ctcctcccgg cacccctgcc cgccccggcc tcctgacgac
    11281 ggggttgtca agcccgcgtc acgggctaac ctcgactcaa cggttcctca gggagagacg
    11341 agatgaccac caaccgacgc acggcgctgc tcggcgctgc caccatcgcc cttgccgcca
    11401 ccatcgccac gcctgccgcg gcgcaggagc agtccttcat cctcaccacc gccacgaccg
    11461 gcggcaccta ctatccggtc ggcgtggccc tcgccacgct cgtcaaggtc aagctgcagg
    11521 cttccgacgg catcgacatg tcggccatca gctcggccgg ctccggcgag aacatcaagc
    11581 tgatgcgcga gggccaggcc cagttcggca tcctccaggg cctcttcggc cgctacgccg
    11641 ccaagggctc cggcccgctc gagggcgagg gcaagcagga atggctgcgc tcgatctcca
    11701 tgctctggca gaacgtcgag cacttcatca ccaccgagga cctggccccg accggcacca
    11761 tcgacgacct gaagggcttc gccggcaagc ccttctcgat cggcgccaag aactcgggca
    11821 ccgagggctc gggccgcgag atcctgaacg ggctcggcat cgacccggag agcatggacc
    11881 tcgtctacca gggctacggg cccacggccg agggcctgca gaacggcacc atcgtcggcg
    11941 ccaacatgcc gggcggcgtg ccggtctcgg ccgtgaccca ggccttcgcc gcccgtgacg
    12001 acctcgtcat cctcgacttc acagccgagc agatcgagcg ggcgaacggc ggcgacgagc
    12061 tctggaccgc ctatgtgatc ccggcggaga gctatcccgg ccaggccgag gacgccagca
    12121 ccatcgccca gccgaacttc ctcggcgtcc atgccgacat cgacgaggag gcggtctaca
    12181 aggtcaccaa gaccatgttc gagaacctgg gcttcctgca gaacatccac cccgcgacca
    12241 aggccatgtc gctggaggcg gccctggccg gcctgccggc gccgctgcat ccgggtgcgg
    12301 tgcgctacta ccaggaggcc ggcgtcgaga tccccgcccg cctgatgccg aactgacggc
    12361 gggtgtcccg gccgtggcgg cggggccctg ccccgtcgcc ggcgcctgat cgagcccgat
    12421 gaccgagaag ctggacgagg ccggcgagct gaccacgccg acgcgcgcgg gcaccggccc
    12481 cctctacgcc cgcgccgtgc tggcgctgtc ggtgctgatc tcgctcttcc acatctacgc
    12541 caacagcatc ggccggctcg acgacctcct cctgacctcc atgcactggg gcggcttcgc
    12601 cctgctctgc accctcctct accccctgcg accgggaggc ggccgcctcg tcctcggcat
    12661 cgacatcgcg ctcgggctgc tggcggttgc cgccgccgtc tacgtcaact gggcgttcga
    12721 cgcgatctac gaccgcggcg tgcgcttcat ctggtcggac tgggtcatgg cctgcctcgc
    12781 catcgccctg gcgctggaga tggcccggcg cacggccggc ctcttcatcc cctgcctcgc
    12841 ggtcgtcgcc ctcgtctacc tcgccggtgg cggcgcctgg gtccccggca tgttcaagtt
    12901 cccgggcctg acgctcgaga cgacgctctt tcgggcctat gtcgagctgg tcggcatgtt
    12961 cggcccgatc gcccggatca gcgccacctt cgtcttcatg ttcgtgctgt tcggcgcctt
    13021 cctcgtccgc tcgggggccg gcgccttcgt catcgacctc gcccgggccg tcgccggccg
    13081 ggtggtcggc gggccgggct tcgtcgccgt gctgggctcg gcgctgaccg gcaccgtcac
    13141 cggctcggcc gtggccaaca ccgtctcgac cggcgtgatc accatcccgc tcatgaagaa
    13201 ggcgggcttc ccggcgaagt tcgccgccgg cgtggaggcc tcggcctcga ccggcggcca
    13261 gctcatgccg ccgatcatgg gggccggcgc cttcgtcatg gccacctaca cccagatctc
    13321 ctacctcacc atcgtcgccg tctcggtcct gccggccatc ctctatttct tctcggtcgc
    13381 cgtctgggtg cggatcgagg cgaagaagca cgacatcacc ggcgtcgacg aggactcgcc
    13441 gggcgcgctc gacatcctca ggcgcggcgg catcagcttc ctgatcccca tggctgtcct
    13501 gatcgtcatg ctgctgcagg gcttcacgcc aacctatgcg gcggccttcg ccatcctcgc
    13561 ggtgatcggc tcgtcctggc tgacgaagac gcccatgggc ctcctcgcca tcgtcgacgc
    13621 gctggcgctc ggcgcccgca acatggtgat gaccgccatg ctgctggtct cggtcgggat
    13681 catcgtcatg gtcgtcgaga ccaccggcat cggcaacacg ctctcgctga tgctcgccga
    13741 gtgggccggc ggcaatctca tgatcgccct cgtgctggtc gccctcgcgt cgctcgtgct
    13801 cggcatgggc ctgccggtga cggccgccta tatcgtcctc gccaccctct ccgccccggc
    13861 cctctaccag atgatgaacg acgccgcgat cgtcgacatg atcgcccagg gcacccttcc
    13921 ccaggcggcg caggcggtct tcatgctggc cgaccccgac gccatgacca agctgcaggg
    13981 ccccctgccc gcggccgagg cggccgccct ctacgccctg gtgccgagcg acttctcgcg
    14041 caccctctac cagcaggcgc tcgacccggc gctgctcacg accacgctcc tctcggccca
    14101 tatgatcatc ttctggctga gccaggacag caacgtcacg ccgcccgtct gcctcaccgc
    14161 cttcgccgcc gccgccatcg ccaagacacc gcccatggcc accggcctca ccgcctggcg
    14221 gatcgccaag ggcctctaca tcgtccccat cctcttcgcc tacacgccct acctcaacgg
    14281 cgactggctc accggcctcg agatcacctt cttcgccgcc ttcggcatct acgccctcgc
    14341 cgtcggcttc gagggccacg gcgagcaccc catcccccac tggctccgcc cggtcatctt
    14401 cgccatcggc gtcttcctca tctggcccac cggccgcctc tcccagatcg ccgccgttgt
    14461 ggccctggtc gccgtcatgc tcctcgcggc gaagctggcg ccgaagcggg agtatgcgtc
    14521 gccggtcgag acggcctgac gtcgcctcgt caaccggcga ccggcgccgc gtcccgcagc
    14581 acgaagagct tggcacccgg caggctggcg aggcgatcga tcgcctggtc gcgatcctcg
    14641 atgaagtgct cgccggtgga gtgcagcagg cggtagtgca agggccggcg tgtcgcggcc
    14701 ggccgcttcc ggctcgctgc ggtccgttgc gccgacgtcg tcaccttgcg gtccattcga
    14761 ccctcccgat cctggcgatt ccaccatccc ggtcgcgacc accgcgagcc gggatcgtcg
    14821 ccagcatgcc ccttgcccaa cggatcgtaa gggccggaag tttcatgcct agcactcgcc
    14881 ggcgcggcat cacgaatgct tatggctgca tcccccgaag caccgcggcg ttcaccaatg
    14941 gcggccaagc ccacccttca cggctcgggt tgtcgcggat tccgcgacag ttccgctcgg
    15001 gttgtcgcgt ataccgcgac atccagcccg agattgtcgc gccggacgcg ccataagcat
    15061 ccgtgatggc gcggattccg cgacatttcc ccggattgtc gcagattctg caacagccgc
    15121 cgacgaacga acccgaccaa acgacgatcg aacccgagga ttcgctcgaa aaacccacgg
    15181 aatcgacgaa cgaacccggg ctgttgccaa cgaacccggg gccacgcaag tcattgaaca
    15241 aaaggatttt ctatcccctg ggcgaggttt gccgctggcc ccggacccga gcatggcacg
    15301 gattgccgaa cggacccgca acgggcttgc ctcgtgatcg gcaataggtt ataaaaccta
    15361 gaacagagcc cttccggaac cccggccccc ggcagcagtt cgagcatccg ggtgtgggcg
    15421 atcctgcctg atgcagcggt cggtcacgga accggcgcct cgtgagagag gcggcgaaca
    15481 tgcgaaaggg tgccaggatg ccgactgcct tcgtcgagaa tctcgatcgg tccgtgagga
    15541 cggagatgat cgaggaattg aacgcgcgtc tcgtcgaggc gctagacctc gtgctggccg
    15601 tcaagcacgc gcattggaac atcaagggcc cctccttcat cggcattcat ctgctgctcg
    15661 acgaggtcgc ccaacgcatg cgcggccacg ccgacacgat ggccgagcgc tgcgtcatcc
    15721 tgggcgggca ggcgaagggc acggtgcagg tggtggcgga ggagacgacg ctcgaggcct
    15781 acccgcccga gatcacgagg caggaagagc atgtcacggc cctcaaggag cggttgatgg
    15841 ccttcggcaa ggccgttcgc gagtccatct cgaaggccga cgaagccggc gacgacgaca
    15901 cggcggacct cttcaccgcc gtcagccgtg ccgtcgacaa ggatgcctgg ttcatcggcg
    15961 cccacggcga gcgctcatga cggctcctag cccccgccct cgttcgctgg ccggtagaac
    16021 acgtgatcgc cgacccgcgc cgtctcgaca taatcggcgg cccagtcggg cgtgtcgttg
    16081 atgccgtgga agaagagggc gccgtcggtg cgatcgggca gctcctggtt cagcgcccgg
    16141 cgtgccacat cggtggtgat cgcatagcgg tccggctcct cgacatcgtc ggggctgccg
    16201 tcgcaccacc aggcgaactg gcagggcccg ccctccttgc cctcggtcac cacgccgcag
    16261 atcgtgtccg ggaagggctc ggcggcgaga cggttcatga cgacgttggc gaccgcctcc
    16321 atggcggcca catcctcgcc cttggcctcc cagtagatgg tccgggcgag gcaggtcagc
    16381 gggtcctcga gcggtgccgc accctcgggg tcgaccgccg ccgcctcggt cttcgtcaag
    16441 ggctcggctg ccggcgcgcc gctccccccg gccgcctcct cctccagccc ctcgagctgc
    16501 tccagcgtct ggcccgtcgc cccgtcctgc gcccctgccg gagcggcgac ggcgagcgcc
    16561 aggagaaggc cggcagcgat ccaagggccc ggcatcacgc gccacccgca ggctcgtcgc
    16621 cgccccgcca gaggcgggtg agcgggccgt cagcgccttc gaccgggtcg gtctcgggca
    16681 gcgccacggc agcgcagcgc ccgaccaggg atgtcatgct ctcgccggca gggcgcagga
    16741 cgctcggccc ctcctggccc ggcgggtagc caccgacgac gaggaggtcg gcgctggacg
    16801 aaaggcgctt gtggccggtc cccgccggca ggaccaccac gtcgccggcc cggaccgaga
    16861 cctccggccc gcccgcacca ccgagctgca ccaccgcctc cccccgggcg atgcccagca
    16921 cctcgtgcgc gtcgtcgtgg aaatgctggt agggaaagat gccgttctgc caggtgccgc
    16981 cccagccgtt ggcccggaac agccgctcca gccgatcccc gagccccggc cctgcctcgc
    17041 aggcggcacg gtagagaagc accggcagcg gcgagttcgg cacgtcatgg cccggctcca
    17101 gcagcagcgt ctcgacctcc gccatgtcgc ttactcctgg aatccgtaga gccgggccgg
    17161 gttgtcgacc aggatccgac ggcggtccgc ctcgtccggc gcccagtcga gcagcgtgtc
    17221 gagcagcatg gcgtcgtcct tgggcgcatg gcccgggtgt ggccagttgc tcgcccagag
    17281 catgcgctcg ggcgcatcct cgaccagcac ccgggccagc cggcccacat cgtcataggt
    17341 cggcgggccg accttcgagg tctcgtaggg tgccgaaagc ttgacataag cgcccgcgcc
    17401 gaccagccgg cgcaggcagg cgaaggccgg gtgatcgaca tcgaccggct cgagatactt
    17461 gccggtgtgg tcgatgacga gttgcacgcc gaggccgagc agggcgtcga catgctccgg
    17521 cagcagccga ccgtcgagct gcacctgcag gtgccagccg aagggcttca ccatggcggc
    17581 gagctcgggc aggtcggccc agtcgtagac cccgcccttg agcatgaaga cacgcagccc
    17641 cctggccccg gcccggttca gccggtcgag ctcggcctcg gcggtgccct tgggcacgac
    17701 cacgatcgcc cgagccgtct cgccgatcac cccgacattg tccatggtga cggtattgtc
    17761 gtaaccgtag cccgccgcct gcacgaccac gacccgttcg aggccgagcc gcttctgcac
    17821 ctcgcggtac tgggccagcg tcgcctccgg cgggctgttc cagctcgtcg gcggcagggg
    17881 ccagcgatca tagggtccgt agaaatgcat atgcgtatcg gtggcgccgg gcggcgtctg
    17941 cagcttcggt ccggtcacga tcggttcctc ccccgggatt cgaccgggcc acactagacg
    18001 gaacgcgcgc gatggtcagc atcgacgaac ggctcgccga ctggccatcc gacctcgcca
    18061 cggcgcgggc gatgcaggag atggtccgcc acctcgtgtc cgaaagggcg ccggccggct
    18121 tcgtgccgcg gctcgtcgcc ggcgtcgatg tccacttcag ccgcgacggc cggaccgcct
    18181 tcgccgccgc ggccgtgctc gacgccggga gccacgagct cgtcgagagc gcccagcttt
    18241 ccctgcccgt cggctttccc taccgcagcg gcttcctctc gttccgcgag gtgccggcgg
    18301 cattggcggt gctgggcctg ctggcggcac gctatgacct cgtgctggtc gatggccagg
    18361 ggcgggcgca tccccgccgc tgtggcttcg ccagccatct gggcgtgctg ctcgaccgcc
    18421 cgaccatcgg tgtcgccaag tcgcgcctga tcggcagctt cgtcgagccg gcccccgagc
    18481 gcggggccgc cagcccgctg cacgacaggg ccgagatcat tggtgccgtg gtccgcagca
    18541 aggccggctg ccggccgctc ttcgtctcgg tcggtcacct gatcgacctc gacggtgccg
    18601 tcgccgccgt gctcgccctt accgggcgct atcgcctgcc ggagccgatc cggcaggcgg
    18661 atcgcctgtc ccgggcacat ggatgagcgc ctcgcccatg gcccgcagga gcgccaccgg
    18721 caccgttggc ccggccgcct cgacatgcac ggtgcgggcc gcatcgtgca ggctggcata
    18781 ggggcgaata ccgtccgcat gccagccgag atgccagccc cagccgggca gggcgtggcg
    18841 gatcaggtcg agcgccgcat cgatcgaggc cgtataggcg gccggtggtg cgccgagctc
    18901 cgcggcgagc cgccggtcca gctcgcggtc ggcgccggtc gcggccagca gctcctcttt
    18961 cagcagcccc atcgcagcgc cggcgtatgc acgggcgcat cccagaggcc ggtcgtctcg
    19021 gcatcgaggc gtgtcaccgt cagcgagcag ccggccatgt cgagcgaggt gcaccagttg
    19081 ccgacgagcg agcgggcgac ggtgagcccg gcggcctcca cccggcgcgc cgcctcgtcg
    19141 tagacgaggt agagctccat gagcggcgtg gcgccgaagc cgttgaccag gagcaggaca
    19201 tcctcgccct tggccgcgcc caggtcggcc aggatggccc ccatcatctc ctcgacgatc
    19261 gccgacgccg aggccagcgc cacccgcctg cggcccggct cgccatggat gccgaccccc
    19321 atctccatct cggcctcgcc gatctcgaag gtggggccgc cggcggccgg cacggtgcag
    19381 ctcgtgaggg cacagcccat cgagcgcgtg gcgccgttga cccgctcgcc gagcgcctgc
    19441 agcgtggcga ggtccatccc cgtctccgcc gccgccccca gcatcttctc gaccacgacc
    19501 gtgccggcga ccccccgccg gccggtcgtg tagctcgagt tctcgacggc gacatcgtca
    19561 ttggtgagca cgcgggcagt ggcacagggc gccatctccg ccgccatctc gaagttcatg
    19621 acgtcgcctt cgtagttctt gacgatgaag aggacgcccg cccccgaatc gacggcctcg
    19681 gcggcggcca gcatctggtc cggcgtcggc gaggtgaaga cctgacccgg gcaggccgcg
    19741 tgcagcatgc cccggccgac gaagccggca tgcagcggct cgtgacccga cccgccgccc
    19801 gagatcagcg ccacctgccc cgccggcgtg acggcgcgcc ggcggacgaa cagaggcgcc
    19861 tggctcaggc tgaccagatc gccatgggca cgggcgaagc ccgccaggct ctcgcccaga
    19921 atggcgtcga catcgttgat gagcttcttc atggccgagc ctcccgcggg cgtgatcgct
    19981 gttctggcgt agcatggaat gcccggggtc cgccaccggg cgccgcgtcg cccggtccgg
    20041 tcaacgggcg gacgatggcg ccagcacgcc gacgaccgca ccgatcagca gcgccgagga
    20101 gcaggcgccg gggtccagat gacccttcga gcgctcgccc aggaacgacg cccggccgcg
    20161 cttcgccacc atgtcggtgg tgcgatgcag cgcgtggccg gccgcggcga tgatccgctc
    20221 gtcgaggcca tcggtctcgt tctggtcgac cagcgagcgc agcgtgtcgg ccaccgggca
    20281 gagcacatcg agcatcgtct tcatgccgac ctccgccttg ccgcgcgcct tgacaccccg
    20341 gatgccggca tcgagcatga ccgacagatc agcgagcgtc ggcgccgcct ccggcgtcgc
    20401 cttgcccatg tcgatgaaga gcgtgccgta gagtggcccc gaggcgccgc cgaccttcat
    20461 caccagcgtc atgcccgcct tcttcagggc ctcgcccagc ggcagcgcgg cgatggccgc
    20521 cttctgcgcc gcgagctcct tgaagccgcg cgccatgttg atgccgtgat cgccgtcgcc
    20581 gatcgcctgg tcgaggcggg tcaactggtc ggcgtggctc tcgatggtgc cagccatgcc
    20641 ctcgatcagc cgcagcacga gcgtgttcga cattggagct cccccgatcc ttgcttgggg
    20701 gcgagcttat cgcacgcacc gcgcgcggca accaccctgc cgttccggct tcaccgctct
    20761 agggctggcc gaataacggt ctcaggcgga caccgatcat gctgccggca aaggcggcga
    20821 cgagccagac ccagccatga aggctgcccg aggctatgcc cgagaagaag gcgccgatgt
    20881 tgcagccgaa ggcgagccgc gcgccatagc ccatgagcag accgccgagc acggcgctgc
    20941 cgatgtcgcc cgcaccgagc cgcagcgtcg gccgaaacct gccgccgagg ccggctgcca
    21001 gcatggcgcc gagcacgatg ccgaagttca tcaccgaggt gacgtcgaag agaaccgagc
    21061 gctcgatggc gccggccatg ccgccccagt tgtgccatgc cgcgatctcg acccccaggg
    21121 catccagccc cttggcgccc cagagcgcga aggccgaggt gatcccccag ggccgtcccg
    21181 ccagccagag cgtgaggaag ttgaggactg ccagtgccgc ggcaccggcg aggatcggcc
    21241 atggcccgcg cagccagtcg ccggccgtcc gcggccgact cgacacgccc cgacgccgct
    21301 cgacctggag cgtcagcgcc acgatcaggg cgaagagagc aaggtggagc agcagcgccg
    21361 gccagaggcc gagcatggtg atcgccgaga ccccgccgat gcccggcagc ccgtgccaga
    21421 acggcagatg gccgatggcg acgaccgagc cggcgacgaa gaagaagagc gtcagcacca
    21481 tgcgcgtgct gccgccgccg accgtgaaga gcgttcccga gccgcagccg ccgccgagct
    21541 gcatgccgat gccgaagaca aaggcgccgg cgaccacgcc gagaccgacc ggaaagacga
    21601 agcccccgac cgggcggccg aagagctcac cggcggcgag agccgggaag aagaggcaaa
    21661 cggccagggc cagcatcagc atctgggcgc gcagcccatg accttcgccg gtcgtcacga
    21721 accgccgcca ggcggcggtg aagccgaagc tcgcatgatg gagaacgagg cccaggagcc
    21781 cgccgaccac ggccagcgcc gccatgttcg gccctgccgc ctggccggcc tgccagacga
    21841 gcacaaccag cagcagcagt gcagcgccga cgatccggcc gtcgatggtc acccccgacg
    21901 tgccgacacc cattgcccgg ctgctcaacg tgaattatcc aaaaggcatc tctgccctcg
    21961 tagatgggca cggcagtcgg gcctgcaaag ggtcagtcgc tctcgtccgg ctcggcccgg
    22021 ctgatcgtca gcacggacag ccggttgcgc tcacgcttct tcacggtcac gcggtagcgg
    22081 ccgatcgcca gtgtctcgcc ttcgagcggg atgcgctcgg cgacgtcgat gacgaggccg
    22141 gcgagcgtca ccgcgtcctc ctcgggcaga tcccattcga ggaggcggtt gagccggcga
    22201 atctgagtgg tgccggaggt ctgcaggctg ccatccggca ggcgctcgat ctcgtcgaac
    22261 tcgcgatcat cggcctcgga gacctcgccg acgatggcct cgagcacgtc ctgcagcgtc
    22321 accagcccct gcagatcgcc gtactcgtcg acgacgaagg ccatgtgctg cttcttcttg
    22381 cggaactgct cgagctgctt cctgagcggc gtcgtttccg gcacgaacca gggatcgcgg
    22441 gtgatctcgc cgaccgtgcg gctcgccccc gcctcgcgat cagcgacggc cgtgagcagg
    22501 tcgcgcagat gcaggatgcc gacgatgtga tcgctcgagc cgcgatagac cggaaagcgg
    22561 ctgtacggct cgtcgcgcag ccgtgccacg gccgcctcga tggtcatcga ggcgtcgagg
    22621 gcggttaccc gacgacggtg ggtcatgacc tcgtcgaccg tgacgtgctc gagatcgagc
    22681 acgccgtcga gcatgtcgcc ggcgtgcttc gcaagctcgc caccggcctc gaaggcatcg
    22741 atcaggccgc gtagctgctg gctcgccgag atgacgtcgc cgccatgggt gggaatgcgc
    22801 aggccgcgga gcacgagcct gaccacccag ccgatggccg tggtgaaggg gcgaaacagc
    22861 acgacgagga cgcgcaaggg gccagcgatg gccagggcga gcgtgtcgtg gtggcggagc
    22921 gccacggttt tcggcacgac ctcggagaaa atgacgatca gcagggtcat gacggcggtg
    22981 gcatagacca ccccggcatt gccgaagagc cgcagcagca cgtcggtcgc gagcgctgcc
    23041 gccatgatgt tgacgatgtt gttgccgagc agcagcgtgc tgatcagccg ttcgcgatca
    23101 ttgagcaggc tgaggagcgt accggcgcgg cgattgccgc cctgggcgag cgcggtggcc
    23161 cgtgccctac ccgtggcggt gacggcagtc tcgctgcccg aaaatgccgc cgacaggagc
    23221 agcaggatca ggatcgcgac gatgtacaac gtgatcaagg acttgtcccg gcggccgacg
    23281 gctggcctga gccatcgcag ttagcctgtc ggcgtcgacg aatcaatctg ccggccgcac
    23341 catgccgcag gcgaagcccc attcctactc ggccgcctgc tccggcggca tggtgatgac
    23401 cggctccatt tccgcgagca acgccggctc gatatggcag gcgatccggt gaccggccga
    23461 gaattcgcgg atcggcggct cctcggtgtc gcagatggcc ccgagcttgc gcgggcagcg
    23521 ggtctggaac gggcagccgg atggcgggtc gaccgcggag ggaatgtcgc catgcagcac
    23581 gatgatcttc ttccggatcg acgtgtcggc gatcggcacc gccgacagca gcgcctcggt
    23641 ataggggtga tagggcggcg cgaagacctc gtccgtggtg ccctgctcga cgatcgtccc
    23701 gagatacatg acgaccaccc ggtcggcgag atagcgcacc agcgagaggt cgtggctgat
    23761 gaagatcagc gtggtgcggt tctcccgctg gatgtccatc aggagctggc tgaccgccgc
    23821 ctgcaccgag acgtcgagcg ccgagaccgg ctcgtcggcg accaccaccc gggggttgcc
    23881 ggcgaaggcg cgggcgatgc cgacccgctg cttctggccg cccgagagct ggcgcggccg
    23941 gcgcttgtag aagtcccttg gcagcttgac gatgtcgagc agctccatca cccgggcctc
    24001 gaccttggcc tggtcgctct cgacgccgaa cttcctgatc acgcgggcga tctgcccacc
    24061 cacggtgtgg ctcgggttca gcgtgtcgaa gggattctgg aagaccatct gcagatggcc
    24121 gacgacctgc ggccggcgcc gctccacggg cgtctgcgcc aggtccttgc cgtcgaggat
    24181 gagcttgcct tcggtggcgg tctcgagacc catcatcacc ttggcgaagg tcgacttgcc
    24241 gcagccggat tcgccgacga tcgccaccgt ctcggcctcg cgcgcctcga aatcgagctt
    24301 ctcgttggcc ttcaccgtgc gcaccgagcg gccactgatc agggccatga gcgaggggtc
    24361 gcgcagctcg tagtacttct tcatgttctc gacgcgcagc accacctcgc cgagattggc
    24421 ggaagctgcc gcctcgacca cctccggccg ggcctgccag tcgatctcgc tgaaccgtgc
    24481 gcagcgcgtg tagtgccgct ggtgcgccgg caccgtcgcc atggcgatcg ggcccgcatc
    24541 gcagacgccc gcgcggaaat ggtcgcaacg cgggccgaaa ttgcagcccg gcggccgctc
    24601 gtgcggcagc ggcagctggc ccgggatggg catcaggggt cgcgcgtgct tgtcggcacc
    24661 gggcagcggg atcgagttga agaggccctt ggtgtaggga tggcgcatcc ggtcgaagac
    24721 ctgctcgacg ctgccctcct cgaccgcctc gcccgagtac atcaccgtga tccggtcgca
    24781 ggtctcgagg atcagcccga gattgtgcga gatgtagatc atcgaggtgc cgtaacgctc
    24841 gctgatctcc cgcacgagct ggacgatgcc ggcctcgacc gtgacgtcga gcgccgtcgt
    24901 cggctcgtcg agcaggagca ggctcgggtt cgagagcagc gccatggcga tcaccacgcg
    24961 ctgctgctgg ccgcccgaga tctggtgcgg gtaggcgtcc atgatgcgcg cgggatcggg
    25021 cagccggaca tcggccagca tctccagcgc acgctggcgc gcatcggcct cgctgcagcc
    25081 ggcctcgtga tagagcggca cctccatgag ctgccgggcg atggtcatcg acggattgag
    25141 cgacgccatc ggctcctggt agaccatggc gatcttcgag ccacgaagct ggcgcagctc
    25201 ctcctgcgac atcgtcgtca ggtcctgccc catgaagcgg atctcgcccg acacgatgcg
    25261 gccgttgccg ccgagatact gcatgatggc gagcgccacg gtggacttgc cgcagcccga
    25321 ttcgccgacg atgccgaccg cctcgcccgg catcacgatg agatcgaagt cgaccaccgc
    25381 cgggatctcc ccggcccggg tgaagtagga gatgcagagg ttcctgcact cgaggatcgg
    25441 cgtcgcgtcg gccatcttgc tcaatccctg aggctcatct cgcgcagacc gtcggccatg
    25501 aggttgaagc ccaggatcag gctggagacg gagagggccg ggaccagcag catgtaggag
    25561 aacttgaaga gcatggcggc ggccgccgcc tccttgatca tcagccccca gtctgggtcg
    25621 ggcggctgca ggccgaggcc gaggaaggtc agggtggtga tcgccacggt ggtgtagccg
    25681 agtcgcaggc aggcatcgac gatgatcggg ccgcgcgcgt tgggcaggag ctcgaccacc
    25741 atgatccaga gcgggcgctc gccgcgagtc tgcgccgcga agacatagtc gcgcgtcttg
    25801 atgtcgagcg tcaggccgcg gacgatgcgc atgatggccg gcgctgaggc gaaggtgacg
    25861 gcgacgatga tgttgaagcc ggacgggccg aaataggtca ggatgacgat gaacagcacc
    25921 atcaccggaa acgacaggat gacgttgccg atgaacgaga tgacctcgtc ccaccagccc
    25981 gccagataac cggcgacgag gccgagcgcc gtgccgatga cataggcgac ggcggtggcg
    26041 gtgacgcccc agaccagcac gcgctggcag ccccagagcg tgcggctcag catgtcgcgg
    26101 ccgcgaaaat cggtgcccag ccagaagaac atgtcgcgcg cttcggtcag cggcgtgacg
    26161 aagggctgga ccggctggtt cgggtcgagg agcggcagga acggcaccac cacagcgatg
    26221 aagagccaga acatcacgag agtgacgccg atcaccgcga ccgcgctctc ccgccaggag
    26281 atcacggcga gggcgtagag cagcaaggtt gccgggatcg ccgggatcag caccatgcgg
    26341 ccgacgccga acatcacata ggcgacgagc ggcagccaga ggagggccac gaggccgagc
    26401 agcacgcgcg gccggcgcag gcggtcgagc agcgacgggg ccgggctggt cggtgcggcg
    26461 atggtagcca tgtcgcctcc ccctcagccg aagcggatgc gcgggttgag cagggtgtag
    26521 ccgacgtccg agacgatctg cgagagcacg gcgacgatga cggcgacaaa ggtggcggcc
    26581 tgaacgacgt agatgtcacc gaagagcgcc gcctccagca gcagcttgcc gaagccgcta
    26641 tattcgaaga acacctcgac gacgacgacg cccgagagca gccaggtgag ctgcaggacg
    26701 atgaccgtga acggcgctat cagcgcattg cgcagcgcgt ggcgc
//
LOCUS       DAOTFI010000025        26136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2151_length_26136_cov_6.061117, whole genome shotgun sequence.
ACCESSION   DAOTFI010000025 DAOTFI010000000
VERSION     DAOTFI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 26136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26136
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2151_length_26136_cov_6.061117"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="dppD"
                     /locus_tag="P5558_03960"
     CDS             <1..430
                     /gene="dppD"
                     /locus_tag="P5558_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="DppD and DppF are the ATP-binding components of the
                     ABC dipeptide transport system DppABCDF; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein
                     DppD"
                     /protein_id="HRY23498.1"
                     /translation="ALDVTIQAQVLELMKDIRTRLNSAVLLISHDLGVIADLCERVIV
                     MYAGRIVEDADVRSIFKRSRHPYTRALLQSVPSLAAGQKRLYQIPGSVPQPGTGHRGC
                     PFRPRCALAMEVCEREMPPLTSYGSGHRAACWAPAEAVAA"
     gene            462..1454
                     /locus_tag="P5558_03965"
     CDS             462..1454
                     /locus_tag="P5558_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY23499.1"
                     /translation="MKALIEVRDLGKTFHDTSMRIGQKRPPVRAVDAVTLDIRVAETL
                     AVVGESGCGKSTLGRLLLRLIEPSEGTVFFGGIELTTLGREKLRQARQRMQMVFQDPF
                     GSLSPRRTIADIVAEPMDVFGLSGNRRERRNRVAELLEQVGLPAAVMDRKPRQFSGGQ
                     RQRIGIARAISVNPTFIVADEPVSALDVSIQAQIINLMQDLQEQRKFSFLFIAHDLAV
                     VRHIANRVAVMYLGRVVEIGEKEKIYTAPHHPYTQALLSAAPQPDPDLKVERIILQGD
                     VPSPTNIPSGCAFRTRCPIAQDVCAVERPALRQVSQGQEAACHFARPLPLDTAP"
     gene            1451..2479
                     /locus_tag="P5558_03970"
     CDS             1451..2479
                     /locus_tag="P5558_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRY23500.1"
                     /translation="MTGALSGLEVLDLSDTIGGQYCTRLMADFGARVRAIEPPGGTAT
                     RRIGPFDAHGRSLTHLHLSLGKEETPAADMAELRLRADIVVCGAGEGAAFRATNHKAV
                     VTEVSSFGADGPCAHWQAPEIVLQALSGMMNSNGTAGREPLYGVGNRASYAAGVAAYI
                     ATVAALLARRGTGLGDLVRVDSAETAAAMCFPYLQQYFYNGTNRRRGLQDIPAGQVLC
                     RGSWVCIWVYSNRFERLCRALGLEDCLEDPKFADLRERSGNWRGFFERVAAVVRDRDA
                     EDLVAELQGLDIIAARAYRPSELRASRHLAARAYWRRIEIDGARYVIPGPPFRFSATP
                     AIERGVPA"
     gene            2476..3717
                     /locus_tag="P5558_03975"
     CDS             2476..3717
                     /locus_tag="P5558_03975"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRY23501.1"
                     /translation="MSPPLEGVRVVELTTAWAGPFAGRVLAWFGAETFHVESATRTNT
                     WRVNRDAPRAVNYPDLDPGNRPFDRSFLFNSQNVNKRSLAIDLKIPEGLAALRRLLDV
                     SDVLLCNFRPGFLARIGLDRATLAQTNPGIIVLQMPAFGTSGPHAGYAALGPTMEMAA
                     GMSAMIAYPGGEPTVTGPSYLDPIGGFNAAAAVMTALWHRDRTGEGQEIEMGQVECAM
                     QFIGPELIAGEDVEQQGNRVRDMAPHSAYPARGEDEWIVIAATDDEAFAALCRAMDRP
                     ELARNFASLEARKQAETMLDAEIARWTVGQDKHELARRLQAAGIAAAPVNTPADLAGW
                     DYLDARASLVELDHPEAGRHPHMGLPFHAETHRGGARTAAPCFGADNLYLLRDVLKMS
                     DGEIEDALASGAFADRPNAGV"
     gene            3725..4525
                     /locus_tag="P5558_03980"
     CDS             3725..4525
                     /locus_tag="P5558_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRY23502.1"
                     /translation="MVESFKTIARLDANPRYAGSTHDDATARAMGFRAALIPGAFLYG
                     HVTRLAVRAWGMDWLGRGQAEVRFRRPVHDGDKLVVGRSGWREVDGGVRAEAAIRHAD
                     TGEILVLGHIALPDMPLEPPTDLDVIPLPDPIPAATSEQIVTGKRLGTRSRVLSLAEI
                     RQSLADFGEREPIYQEGALAHSGCLVRQTMGDSLSNFRFPEPVVFVSVQVQNYAPLHA
                     GARICSATEIRSVYSRKGRLYFDSEEYLIADGLPVARHLRVNLYGSAE"
     gene            complement(4748..4876)
                     /locus_tag="P5558_03985"
                     /pseudo
     CDS             complement(4748..4876)
                     /locus_tag="P5558_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5715..6704)
                     /locus_tag="P5558_03990"
     CDS             complement(5715..6704)
                     /locus_tag="P5558_03990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY23503.1"
                     /translation="MSAFDAVRLARAIERFEAEAGRDGARRLAILHRGDLVHAGPEAD
                     RAQGVWSITKTILSTTLGLLVDDGRVALDQPVTRVLPELAAAFSDATFRHFASMTSGY
                     RAIGDEAPIKGYAHGPSRSFMQPAPTPQSAPGTTFSYWDSAPNMLALALTRVAGEPLA
                     HLLRRRILEPLGIDLEWGAFLVDGQRVHGGAGNHFGTVQISCLDLCRLGELYRCQGLW
                     HGHRLLSADWCAAATSVQVPAGLALHETPFDGRGCYGFFWWVNGVTASGARKWPGVEA
                     GAYSASGYNNNDLFVLPEEELVVCRLGHDQDFDGAIVDDTYAALLNGIVAARS"
     gene            complement(6701..7774)
                     /locus_tag="P5558_03995"
     CDS             complement(6701..7774)
                     /locus_tag="P5558_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23504.1"
                     /translation="MPPRTEIGIDGSDFRINGAPTYAGRWHEGRRIEGLLLNSRMVQA
                     IFDDANPRTRPNWAYPDTGIWDPDRNTRELCAMLPVYRAHGLLAITVGLQGGGSIYRP
                     EIFEHYRNSAFTPEGELEPAYLERLLRVLEAADRSGMVVIVNYFYRKQQRFQDDAAVC
                     RATEAATQWLLESGHRNILVDIKNEIREADDILSSRGIHRLLDIARSTTQDGRRLLVA
                     TSTHPEKHLPDGSWPALCDFFMPHGNNSEPDDFRRELLAFREEPVVAERARPICCNED
                     SIHVASLDAAIDAGASWGYYDQGYGSVGYHGRHDWSARDREADHAELSGFQTVPVNWS
                     INTAHKRAFFDRVKEVTGAGATP"
     gene            complement(7777..9198)
                     /locus_tag="P5558_04000"
     CDS             complement(7777..9198)
                     /locus_tag="P5558_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY23505.1"
                     /translation="MDGLVEIEAAWREARGGYEADIDRHFERYVEELKELCRLPSLST
                     DLAALGDCASYLRDRLDALGFETAIEDVNGPGSAPIVLARRHRDDALPTLLIYGHFDV
                     QPVDPESAWTNRPFDPTEREGYLYGRGTADDKGQFLAHLKAVEMIDRLGAGPVANLIL
                     VLDSQEEVGSPHLAAFVERNRAKLAADYVFYADGEAFVGNRIMIQHGNRGDCFITIRH
                     RSGARDTHSGTYGGVVPNAANQLVAFLATLVDENGVVQIPGFYDDVVPPTAADREAMA
                     RLPHDQAAFERMLGLSRPFGPAGLDHWEKIMFRPTLNISGLTAGFQGAGTKTVIPCEA
                     TAKIDMRLVKNQTPEDILAKFRRHAAAMGMGGLEIEKGMQYLPARTAIDHPYCLLVRR
                     ALGEGFDQEPVVTPVVGGSNPNHVWMERLGLPMAEVSYGQHDCRMHAPDERYDLDHYR
                     HGILTSALVMTGVARLPSRQQGD"
     gene            complement(9223..9921)
                     /locus_tag="P5558_04005"
     CDS             complement(9223..9921)
                     /locus_tag="P5558_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY23506.1"
                     /translation="MKGIGDIDATGRRNGRPAFEIALAHLKGKILNNDYPPNARVLQG
                     TIANELKLSRTPIREAIMRLEQEGLVRIVPRHGIEILSLSPDDTREIYELLACLEPKA
                     VALLASRRPTPDQIEPLVDACAAMEAALEIDDRLAWAHADERFHRCLAELSGNSRLRA
                     AIMGFWEQSHRARMFTLRLRPKPVESTREHRAVVEAILAGDADGAERLYRLHRERAQR
                     VILEIVERHGFKRL"
     gene            complement(9918..10895)
                     /locus_tag="P5558_04010"
     CDS             complement(9918..10895)
                     /locus_tag="P5558_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23507.1"
                     /translation="MSGVEPLLELRHVSRRFPIRSGVLSRVSGHIHAVDDVSFSVARG
                     ETLAIVGESGCGKSTTGRCAVRLDLPDDGQVLFEGIDLATVDRRGLRAVRARLQMIFQ
                     DPSAALTSHMRVGEQIAEPLRNLAGLTDAAEVERRVAALLTTVGLGAADAGRFPHEFS
                     GGQKQRVCIARALASDPVMIVCDEAVSALDVSIKAQIVNLLIEIQERRGVGYVFISHD
                     LGIVESISHRVAVMYLGKLVELADTAALFDAPRHPYTEALLAAAPVIEAAGTRPERPR
                     LEGDVPSAASPPSGCRFRTRCPLATPLCAEAEPPLARRDDGRLLACHHR"
     gene            complement(10892..11902)
                     /locus_tag="P5558_04015"
     CDS             complement(10892..11902)
                     /locus_tag="P5558_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23508.1"
                     /translation="MTALQESTNVLEVENLGIAFGSSESRLSVVEDLTFAVRSGELLA
                     LVGESGCGKSVTALAIMGLLPRPQASTPAGRIVFEGQDITTLDEAAMQDLRGRRIAMI
                     FQEPMTALNPVMRIGRQIEEVLLRHRVVERGEARPRALELLRQVRIADPERRLRQYPH
                     ELSGGMRQRVMIAMALACDPVLLLADEPTTALDVTVQAQIMELIADLQRRRGTSVVLI
                     THDLGIVAETADRVIVMYAGRVVETASVRTVLEAPRHPYTQGLIASVPRLGRNRRGSG
                     APLADIPGSVPPPGKRGRGCVFRQRCAWAIDACAERQPQLDPFGEGHRVACLRAHETV
                     SS"
     gene            complement(11906..12781)
                     /locus_tag="P5558_04020"
     CDS             complement(11906..12781)
                     /locus_tag="P5558_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23509.1"
                     /translation="MATADATALSSPRHGAVRRGLGAIFTRPGFRPGLVLFSLPLLGI
                     LLGALVLAPAGMDGAIRLTAPSWAHPFGTDNLGRDLFAHVLRGGRVSLAIGIAVAVLS
                     TAIGLVLGLLAGFIRVVDAVLMRIMDGIMAIPGILLAIALMSVAGAGLVNVIVAITIA
                     DVPRVVRLVRAMVLSLREQTFVDAARIAGNRTGRIVFRHLLPMTLGPLAVQATFICTS
                     AIITEAYLSFIGAGTPPDMPSWGNIIAENRIYFRGAPWSVLIPGCFIAATVLGINLMG
                     DALRDALDPRVAKAI"
     gene            complement(12790..13731)
                     /locus_tag="P5558_04025"
     CDS             complement(12790..13731)
                     /locus_tag="P5558_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23510.1"
                     /translation="MAAFIVRRMLLTIPVMLVVATVVFLIMRLGASDPAAVLAGELAT
                     DAQIAAIRARYGFDAPILVQLVKWFGLLLQGDLGVSIYTQMPVTTLIGQRIGATLSLA
                     LYAMTIAVAIAVPLGTIAAWKAGSLIDRAVMVFAVSAYSFPVFLISYALILVFALDLG
                     LFPVQGYKPLARGIGPWLEYLTLPALSLGLAYAALLARMTRASVLEMLRQDFVRTARA
                     KGLRTFQVLVRHALRNAAVPIVTVIGLSLAALISGVVITETVFAIPGIGRLTVDGIFT
                     RDYPVVQGVVLFFSFVYVFLNLAVDVIYALVDPRIRY"
     gene            complement(13747..15333)
                     /locus_tag="P5558_04030"
     CDS             complement(13747..15333)
                     /locus_tag="P5558_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23511.1"
                     /translation="MNASTRRLLATTIIAFGSLGPLANANAETVLRVGTSTDVQVVDP
                     MFTTAHPTRDHAYLVYDTLLAVDSNFEIKPQMLESYTVADDGMTIEFKLRPGLAFHDG
                     APVTSADVIASIQRWAKADTMAQIAITYVDDITATGGDSFVMTMKSPSTLIIDAMAKP
                     SGNVLFIMPERFAQLPNTEQVLESIGSGPFTFDRAEWVPGQTRIYRRNESYVPREDPQ
                     DNLAGAKVPGVDVIEYTIMPDDATRQAALLNDEVDYLGNMAHDLVDELRADPDLVVET
                     RDIRGEQLWLRFNHLVPPFDDQKLRQAIYWAVTQDEYMGSLIPDPELWKTCYSMYGCG
                     TEYSTEVGSEPLQAQDLEKAKALVAESSYAGQPIVFLDTTNHPLLHGANIRTAETFRQ
                     IGLEVELQGIAVATLHQRRAIKTPVDEGGWNLFHTTFATIDVFNPLVNPAINASCAQD
                     NWPGWPCSEEIQQLRQDFAAAKDADEKQRIATEVQRLAFELGTHVPLGVFYKVDAWNN
                     RLKGVGEASVAHLWGISIEE"
     gene            15448..15798
                     /locus_tag="P5558_04035"
     CDS             15448..15798
                     /locus_tag="P5558_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY23512.1"
                     /translation="MSHGSETFLGSNSRLNLGGQIVEELGQRIASGYYGAAGVLPNEA
                     ALSDELSVRSSIIREAAKQLSAKGLVEIRSRTGTRVLPQDRWNLLDISSAANQQAWPE
                     SRRDGAKRPKRVRD"
     gene            16099..16272
                     /locus_tag="P5558_04040"
                     /pseudo
     CDS             16099..16272
                     /locus_tag="P5558_04040"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(16329..17189)
                     /locus_tag="P5558_04045"
     CDS             complement(16329..17189)
                     /locus_tag="P5558_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23513.1"
                     /translation="MAKERATGREEPRLPDYGRCPKLLVGQKALVTGANSGIGRAVAI
                     GLARAGADVVVNYVTNEPAAREVVRQIEAEGVRGLALEADVSKEAEVEAMFQAAIEAF
                     GTLDILVPNAGLQRDAALTEMTLAQWETVIGVNLTGQFLCIREAVKEFRRRGVRPEVS
                     VAAGKIVCMSSVHEVIPWAGHVNYAASKGGIQMMMKSLAQELAPHRVRVNAIGPGAIR
                     TPINTKAWDTPKAYAELMKLVPYGRIGEPEDIAQVAVFLASDMADYINGVTLFVDGGM
                     TCYPGFATGG"
     gene            complement(17437..19068)
                     /locus_tag="P5558_04050"
     CDS             complement(17437..19068)
                     /locus_tag="P5558_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRY23514.1"
                     /translation="MIEEVAGVADRSRIDCDVCIVGAGAAGITVALELEATGARICLL
                     DAGGLAYRRRAQALLDGEVVGDSYPPLKETRFAGLGGATRIWGGWCRPLDPIDFTERS
                     WVPNSGWPFGHDSLRPHYQRAHAICGLGEVDYDPPAWAKRFGAEHLKLAGDDVVTTMF
                     QVSPTRFGSRYRAALAATPNIRVLLGAVALRCRAAPTSDRLERVEVGALDGRRFAVAA
                     RLFVLATGGIENARLLLLSGPTPETSIGNARGLVGRYFTEHPFMEPGSFLPRDPAISL
                     RFYFPARTDDMPPDANVRAALSLGRPALEREGLLNAAVFFLPAYEAHPVFTDPAVKAM
                     LELWEKVRGRGVPGGNGDPVRRALRAPHRLVHAAWRRIAVCPGRTSSWRLRAFFEAES
                     LHHNRVRLAAGKDALDRPLPRVEWRLSERDLASMRRVLALHDQALAGAGHGRLALAFD
                     DTAEAWRAAAFGGKHHMGTTRMHASPSAGVVDADCRVHGVRNLYVAGSSVFPTSGFAN
                     PTLTIVALAVRLAEHLKGRLRDQEPSREARPTTTG"
     gene            complement(19065..20627)
                     /locus_tag="P5558_04055"
     CDS             complement(19065..20627)
                     /locus_tag="P5558_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY23515.1"
                     /translation="MTQPRTDQRVLMLGLDAAELTLIERWIAEGRLPALAELARRGRF
                     GPLTSPAADYAGGVWPTFYASRPVARHGIYHNKLWRASTMRIEVPTDAWLGSRPFWEH
                     AAARGLRACIVDVPMVLGPPRPLDGLYLGGWGTHDVIARGSWPPELWRELENRHGAPA
                     MPPEHFGPQDASSLTRLRDSLLDATAQQQQILLDLLGREQWDLACVVLGATHRGGHYL
                     WDLGEIDQAPLDAARRTALEGALAELYVAVDRTVGEIVARVDDATTVLAFAVHGMGPN
                     PGWADLFADILDAALQAADNRPPKRGLLYSLKRRIPFHWARPILTRLPPAVTGRLVEL
                     WSSGMYDWRTTRHFPLAMDHAGYLRLNVAGRERDGIVEPGAAYTALCDRLEGLILALR
                     DAGSGAALAAAVHRAWQEAPATADGRDVLPDLVVTWAGLPARQVRRVVCDQVPGFAYD
                     VPQRLPSGRSGNHRDRGWLIAAGPGIAPGALEPGHSILDLVPTVMARLGVEADPAWDG
                     RPIDLSPGPALP"
     assembly_gap    20883..20982
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20983..22300)
                     /gene="zwf"
                     /locus_tag="P5558_04060"
     CDS             complement(<20983..22300)
                     /gene="zwf"
                     /locus_tag="P5558_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRY23516.1"
                     /translation="MVVLVGATGDLSRRKLLTGLFHLCSEGFIPGCRIIGVSLDEIDA
                     DAFRTIARDALAKFYPRPVAEADWSAFAPCLDYVPMSAGAGALKKAVQAAERGLGGES
                     RRMHYLSVPPSAALPAVRLLAEAELTKSCRIIMEKPFGIDVESAVSLNGQLHEHFTEE
                     QIFRIDHFLGKEAAQNILAFRFANGLFEPIWNRNFIDHVQIDVPETLGLAMRAGFYEQ
                     TGAFRDMVVTHLFQILAFMAMEPPTALEPKPISEEKNKVFRSMLPLDPRHVVRGQYVG
                     YRDEDGVDPDSDTETFIALKCGIDNWRWAGVPFFLRTGKRLAEGQRIISIAFREPPKS
                     MFPPGSGVGVQGPDHLTFDLADSSKLSLSFYGKRPGPGMRLDKVSMQFAMHETGLIGD
                     VLEAYERLILDAMRGDHTLFTTAEGIERLWQVAVPLLESPPPVRLYE"
     gene            22876..23634
                     /locus_tag="P5558_04065"
     CDS             22876..23634
                     /locus_tag="P5558_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRY23517.1"
                     /translation="MAKAKERLDQLLVAQGLAPTRAKAQALILAGEVFRGEERLDKPG
                     TSLDPATELRLKTTSPTYVSRGAFKLIAGLDRFAIDPAGLLCLDIGASTGGFTEVLLE
                     RGAARVVAVDVGHGRLDWRLRNDPRVVVLEKTNARHLTADLLPGAPSLIVCDASFIGL
                     RTVLPAALGLAAPGAWLVALIKPQFEAGRAAVGKGGVVRDAAIHEAVCTDVRDWLATQ
                     AGWTVLGIEPSPILGPKGNREFLIAARRPAARNS"
     gene            23666..24565
                     /locus_tag="P5558_04070"
     CDS             23666..24565
                     /locus_tag="P5558_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRY23518.1"
                     /translation="MAEPPTTLPPFAALRAFQAAAIHGRFKDAAVSLGVTESSISHQI
                     RRLEDFLHVALFDRSAAGVQLTAAGRRYFAEIDPAIRRIQAATSGLLRPSGRSVVRLT
                     LPPSLAVSWLIPQISAFESEHPGIELQLVATTRVIDLARDQVDLAIRHGRGAWPGVEA
                     ELLLDERALPVAAPGYLEPAPGETPSEALGRVRLIVSARFPNEWEEWARARGLPPPSQ
                     QGAIGLDGIEQALQVAESGHGVAMGRRPVVDEHLARGALVAPFGEGDLTGAAYFLCRP
                     PDIEVTSAVRQVQRWLHGLARRT"
     gene            24682..25569
                     /locus_tag="P5558_04075"
     CDS             24682..25569
                     /locus_tag="P5558_04075"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HRY23519.1"
                     /translation="MTIVLNKSGILSESGSLAAPARHPANVTAELTFISPSAARPVLI
                     SSAVTGGAPDERFGTERHAVRVEDMRSLAGALSLDHEGFELHRAPTAVADLYDDAAVE
                     RTYHAETEALLRRVTGASRVVIFDATRRSDAAGGAANRDGSRGPAGRVHVDYTARSGP
                     KRLADLVGADEARRLAATGVRAAQINVWRPIRGPVRRSPLALADASTVRPQHLVATDQ
                     IFPDRVGEIYHLRHDPSQRWYHAPLMTADEVLLIKGWDSLEDGRARFTPHSAFALADT
                     TAATPARESIELRAYVIFA"
     gene            complement(25601..>26136)
                     /locus_tag="P5558_04080"
     CDS             complement(25601..>26136)
                     /locus_tag="P5558_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRY23520.1"
                     /translation="MMGLITCTAELEPGIRMAYAELGGAPGPEDCYLALRGLRTLATR
                     LERHQKSALKVAEWLAGRPEVARVFYPALPDDPGHALWRRDFSGASGLFGFLLQPVER
                     PGLAALLDHLELFGMGYSWGGYESLLIPTNPAPLRTATDWPHEGRMMRIHVGLESPDD
                     LIADLEAGFVRLGAASS"
BASE COUNT         4051 a         8801 c         9171 g         4013 t
ORIGIN      
        1 tgccctcgac gtcaccatcc aggcgcaggt gctggagctg atgaaggaca tacgcacccg
       61 gctcaactcg gccgttctgc tgatatcgca cgatctcggc gtgatcgccg acctctgcga
      121 acgggtcatc gtcatgtatg ccggtcgcat cgtggaagac gccgatgtcc gctcgatatt
      181 caagcgttcc aggcatccct acacgcgcgc cctgttgcag tcggtaccga gcctggccgc
      241 cggacagaag cggctctacc agataccggg ctcggttccg cagcccggca cgggccatcg
      301 gggttgcccc tttcgcccgc gctgcgcgct cgccatggag gtctgcgaac gcgagatgcc
      361 gccgctgact agctacggct ccggccaccg tgccgcctgc tgggctcccg cagaagcggt
      421 cgccgcatga gacgggctgc attgtcgggc gccggcgcgc gatgaaggca ctgatcgagg
      481 ttcgcgatct gggtaagacc tttcatgaca cctcgatgcg gatcgggcag aagcgtccgc
      541 ccgtccgcgc cgtggacgcg gtgacgcttg atatccgtgt cgccgagacg ctggcggtcg
      601 tgggcgaatc cggctgcggc aagtccaccc tcggccggct gctgctgcgg ttgatcgagc
      661 cgagtgaagg caccgtcttc ttcgggggta tcgagctcac cacgctcggc agggagaagt
      721 tgcgccaggc acggcagcgt atgcagatgg tgttccagga cccgttcggg tcgctctcgc
      781 cacgccgcac catcgccgac atcgtggccg agccgatgga cgttttcggc ctgtccggca
      841 accgccgcga gcggcgcaac cgcgtcgcgg aactgctgga gcaggtcggc ctgcccgctg
      901 ccgtgatgga ccgcaaaccg cgtcagttct cgggtggcca gcggcagcgg atcggcatcg
      961 cccgtgcgat ctccgtcaat ccgaccttca tcgtcgccga cgaacccgtc tccgcgctcg
     1021 acgtctcgat ccaggcgcag atcatcaacc tgatgcagga cctgcaggag cagcggaagt
     1081 tttcgttcct gttcatcgcc cacgacctcg ccgtggtgcg ccatatcgcc aaccgggtgg
     1141 cggtcatgta tctcggccgg gtcgtcgaga tcggcgagaa ggaaaagatc tacacggccc
     1201 cgcaccatcc ctatactcag gctcttctgt ccgccgcccc gcagcccgat cccgacctga
     1261 aggtcgagcg catcatcctg cagggcgatg tgccgtcgcc cacgaacatt ccctcgggct
     1321 gcgcctttcg cacccgctgc ccgatcgcgc aggatgtctg tgccgtggag cgtccagcct
     1381 tgcgccaggt atcgcagggc caggaggcgg cctgtcactt cgcccgcccc ttgccactgg
     1441 ataccgcacc atgacgggcg cactgagcgg gctcgaagtg ctggacttgt ccgacaccat
     1501 cggcgggcag tattgcaccc ggctgatggc cgattttggt gcccgtgtca gggccatcga
     1561 gccgccgggc ggcaccgcga cacgccggat cggcccgttc gacgcgcacg gccgatcact
     1621 cacccatctg cacctctcgc tcggcaagga ggagacgccg gcagccgaca tggccgaact
     1681 gcgcctgagg gcggacatcg tcgtctgcgg cgccggcgag ggcgcggcgt tccgcgccac
     1741 gaaccacaag gcggtcgtca cggaggtcag ctctttcggt gcggatggcc cgtgcgcgca
     1801 ctggcaggcg cccgaaatcg tccttcaggc gctttcaggc atgatgaact cgaatggcac
     1861 ggccgggcgc gaaccgctct acggcgtcgg caatcgtgcc tcctatgccg cgggtgtcgc
     1921 cgcctacatc gcgacggtgg cggccctgtt ggcacgccgc gggaccgggc tcggtgacct
     1981 ggttcgggtc gacagcgccg aaaccgccgc ggcgatgtgc ttcccctatc tccagcaata
     2041 tttctacaac ggcaccaacc gccgccgcgg tctgcaggac atcccggccg gccaggtcct
     2101 gtgccggggt tcctgggtct gtatctgggt ctattcgaac cgcttcgagc gtttgtgccg
     2161 cgcgctcggt ctcgaggact gcctcgagga ccccaaattt gccgacttgc gcgagcgcag
     2221 cggcaactgg cgcggctttt tcgagcgggt cgccgcggtc gtgagggacc gcgatgcgga
     2281 ggacctggtg gccgaactcc agggtctgga catcatcgcc gcccgtgcct accgcccgtc
     2341 cgagctccgc gccagccggc accttgcggc ccgcgcctat tggcgacgta tcgagatcga
     2401 tggtgcgcgc tacgtcattc ccggtccgcc gttccgcttc tccgcgacgc ctgcgatcga
     2461 acgcggggtg ccggcgtgag cccgccgctg gaaggcgttc gcgtggtcga gctcacgacg
     2521 gcctgggctg gtccgttcgc cggtcgcgtg ctggcctggt tcggtgcgga gacgttccac
     2581 gtcgagagcg ccacgcggac caacacctgg cgggtcaacc gggatgcgcc gagagccgtc
     2641 aactatccgg atctcgatcc cggcaaccgg ccgttcgacc gctcgttcct gttcaattcg
     2701 cagaacgtca acaagcgctc gctggcgatc gacctgaaga tccccgaggg gctcgccgca
     2761 ctgagacggc tgctcgacgt ctcggatgtg cttttgtgca atttccggcc cgggttcctg
     2821 gcccgcatcg ggctcgaccg ggcaacgctg gcgcaaacca acccgggcat catcgtcctg
     2881 cagatgccgg ccttcggcac cagcggcccg cacgcgggct acgccgcact cggcccgacg
     2941 atggagatgg cggctggaat gtcggcgatg atcgcctatc cgggcggtga gccgacggtg
     3001 accggaccca gctatctcga tccgatcggc ggcttcaacg ccgccgcggc cgtcatgacg
     3061 gcgctctggc accgcgatcg caccggcgag ggtcaggaga tcgaaatggg ccaggtcgag
     3121 tgcgcgatgc agttcatcgg accggagctg atcgccggcg aggacgtcga acagcaaggc
     3181 aaccgggtgc gcgacatggc tccgcacagt gcctatccgg ccagaggcga ggacgaatgg
     3241 atcgtcatcg ccgcaaccga cgacgaagcg ttcgcggcgc tatgtcgggc catggatcgc
     3301 ccggagcttg cccgcaactt cgcctcgctg gaggcgcgca agcaggcgga gaccatgctc
     3361 gatgccgaga tcgcacgctg gacggtcgga caggacaagc acgagctggc ccgccgcctg
     3421 caagcggccg gcatcgccgc ggcgccagtg aacaccccgg ccgacctggc cggctgggac
     3481 tatctcgacg cacgtgcatc cctcgtcgag ctcgaccatc cagaggccgg tcgccacccg
     3541 catatgggac tgccattcca cgcggaaacg caccggggcg gtgcgcggac cgcagcaccc
     3601 tgcttcggcg ccgacaatct ttatctgctc cgcgacgtcc tgaagatgtc cgacggcgag
     3661 atcgaggacg ccctcgcctc cggtgcgttc gccgatcgcc cgaacgccgg ggtttgacat
     3721 ggacatggtc gagagcttca agaccattgc acggctcgac gccaaccctc gctatgccgg
     3781 ctcgacccat gacgacgcga ccgctcgagc gatggggttc cgggcggcgc tgatccccgg
     3841 cgccttcctc tacggccacg tgacgcgtct ggcggtacgt gcctggggca tggactggct
     3901 agggcgtggt caggccgagg tgcgtttccg tcgaccggtc catgatggcg acaagctggt
     3961 cgtcgggcga tcgggatggc gcgaagtgga cggtggggtc cgtgccgagg ccgccatccg
     4021 gcacgcggac accggcgaga tcctggttct tggccatatc gccttgccgg acatgcccct
     4081 tgagccgccg acggacctgg atgtcatccc gctgcccgat ccgatcccgg cggcgacatc
     4141 cgagcagatc gtcaccggca agcgactggg cacgcgcagc cgcgtgctgt ccctggccga
     4201 aatccgccag tcattggccg atttcggcga gcgggagccg atctatcagg agggtgcgct
     4261 cgcccattcc ggctgcctgg tgcggcagac gatgggcgac tcgctgtcca atttccgctt
     4321 ccccgagccg gtcgtgttcg tctcggtcca ggttcagaac tacgcaccgc ttcacgccgg
     4381 tgcacggatc tgcagcgcga cggaaatccg gtccgtctac agccgcaagg gtcggctcta
     4441 tttcgacagc gaggaatatc tcatcgccga cggcctgccc gtggcgcggc atcttcgggt
     4501 caatctgtat ggcagcgcgg aataggaggc gtcgtagcgc cctggtcccg gttcggcgag
     4561 gccccatatg gccgttgaca ggcgggtcgg ctccaccaca tggtcgccaa tcgaccggac
     4621 gaagtgctcg gactacgaag cgctggtgcg gtcggatcgg ggagggccgg tgtcggcggt
     4681 taggggtgca acggtagacg ctcttgccgg tcttgacggc ggcgacgccg agggcggctg
     4741 cgagatgact tttgcgggtg ccgggcgggc cgaggaagtg gaccacctcg atacggccca
     4801 tgaagtcgag ctgggcgagc gccatgatgc ggccccggtc gagcgagggc tggaaggtga
     4861 agtcaaagct ctccagcgtc ttgggcggcg gcgccttgcg gtgctgggga tcgacgcgac
     4921 gctggttctt gccctccagt acggaatggg ccgcggtcgg cgcccacctc tcctcggtga
     4981 agtgctcgtc gacgacgcgg cgcgtcgtgg cgtggacccg cggattggcg aactgggtgc
     5041 gccagtcgtc gaactgggcg ttgaggtcgt cgtcttggcg cggtatggct tgcaggcgcg
     5101 gggcacggcg ccgtaggggt tgagcagcgc cacgagcgag gcgttgtact gcaccacccc
     5161 gttggcgttc tcgccgatca ccgccatctt catccggtcg tggagcagtt cggacggcgc
     5221 gccgtccatg gcggcgaagg cgtcgatgtg gcagcgcagg agccgcttgc cagacaggtc
     5281 cggccaggcc atgacgcggg cgcgcaagca gtcctcgtaa ggcgcgatca gccgcggctg
     5341 cgccggacgc ggcttgtagg ctggcgctgc caagccgacc tcgaggtgcc tgcgcaccgt
     5401 cttgcggtca atgccgagct tgcgcgcgat ggccgagacg ctgagcccct gccgcttcta
     5461 accgatcgtg gatcaagaca atctctccca gtgtgatgac ctgcctgccc cttccgatgc
     5521 gggcctgcag gtggggcatt gccaccgccg ccagtttgcg gcgcacagcc ccggccaatc
     5581 ggtgctccgg cactcggacg ggggactttt caaacggcac ttttggggag cttgcgctcg
     5641 gcactcacag cggttgcagc aggtggactt tcgcaatgcg ccgggcctcc tgctcgtcgg
     5701 cgagaccggg cctctcagga tcgggctgcg acgatgccgt tcagaagagc ggcgtaggtg
     5761 tcgtcgacga tcgcaccgtc gaagtcctgg tcgtggccga ggcggcagac aaccagctcc
     5821 tcctcgggga ggacgaagag gtcgttgttg ttgtagccgc tggccgaata ggcgccggcc
     5881 tcgacgcccg gccatttgcg cgcaccggaa gctgtcacgc cgttgaccca ccagaagaag
     5941 ccgtagcagc cgcgaccgtc gaacggcgtc tcgtggaggg cgagcccggc cgggacctgc
     6001 acactggtcg cggcggcgca ccagtcggcg ctcaggagcc ggtgcccatg ccagaggccc
     6061 tggcaccgat agagctcgcc cagccggcag agatcgaggc aggagatctg gaccgtgccg
     6121 aagtggttgc cggcgccgcc gtgcacccgc tggccgtcga cgagaaaggc gccccattcg
     6181 aggtcgatgc cgagcggctc gaggatgcgg cgccgcaaga ggtgagccag cggctcgccg
     6241 gccacgcgag tgagcgccag cgccagcatg ttgggcgcgg aatcccagta ggagaaggtc
     6301 gtgccgggcg ccgactgcgg cgtcggcgcg ggctgcatga agctgcggct cgggccgtgc
     6361 gcgtagccct tgatcggcgc ctcgtcgccg atcgcccgat agcccgaggt catgctggcg
     6421 aaatggcgga acgtcgcgtc ggaaaaggcc gcggccagct ccggcaggac acgggtcacc
     6481 ggctggtcga gcgcgacgcg gccgtcgtcg acgagcaggc cgagggtcgt cgagaggatc
     6541 gtcttggtga tcgaccagac cccctgtgcc cgatccgcct cgggcccggc atgaacgaga
     6601 tcgccccgat gcaggatggc gagccgtctg gcgccgtcgc gtccggcctc tgcctcgaac
     6661 cgctcgatgg cccgggcgag ccgcaccgca tcgaaggcgc tcatggcgtg gcacccgcgc
     6721 cggtcacctc cttgacgcga tcgaagaagg cacgtttgtg ggccgtgttt atcgaccagt
     6781 tgaccggcac tgtctgaaag ccgctgagct cggcatggtc tgcctcgcgg tcgcgcgccg
     6841 accagtcgtg ccggccgtga tagccgacac tgccatagcc ctggtcgtag tagccccagg
     6901 aggcgccggc atcgattgcg gcatccaggc tcgcgacgtg gatcgagtcc tcgttgcagc
     6961 agatcggccg ggcgcgctcg gccacgaccg gctcttcgcg gaatgccagg agctcccggc
     7021 gaaaatcgtc cggctcggag ttgttgccgt gcggcatgaa gaagtcgcag agtgccggcc
     7081 agctcccatc cggcaggtgc ttctcggggt gggtgctggt ggcgacgagg agccggcggc
     7141 cgtcctgcgt ggtcgagcgg gcgatgtcga ggaggcggtg gatgccgcgc gaggagagga
     7201 tgtcgtcggc ctcgcgaatc tcgttcttga tgtcgaccag gatgttgcga tggccgctct
     7261 ccagcagcca ttgcgtcgcc gcctcggtgg cgcggcagac cgcggcatcg tcttggaacc
     7321 gctgctgctt ccggtagaag tagttgacga tcacgaccat gccggagcgg tcggcggcct
     7381 cgagcacccg cagcagccgc tccagatagg ccggctcgag ctcgccttcg ggcgtgaacg
     7441 ccgagttgcg gtagtgctcg aagatctcgg gccggtagat cgacccgccg ccctgcagcc
     7501 cgaccgtgat ggcgagcagc ccgtgcgccc ggtagaccgg cagcatggcg cagagctctc
     7561 gcgtgttgcg gtcgggatcc cagatgccgg tgtccggata ggcccagttg gggcgcgtcc
     7621 tcggattcgc gtcgtcgaag atcgcctgga ccattcggct gttgaggagc aggccctcga
     7681 tgcgccggcc ctcgtgccac cgaccggcgt aggtcggggc cccattgatt cggaaatcgc
     7741 tgccgtcgat gccgatctcg gtgcgaggcg gcacggtcag tccccctgct ggcgagacgg
     7801 aagcctggcg acaccggtca tcaccagcgc cgaggtgagg atgccgtgac ggtaatggtc
     7861 gaggtcgtag cgctcgtccg gcgcgtgcat gcggcagtcg tgctggccat aggagacctc
     7921 ggccatcggc aggccgagcc tctccatcca gacgtggttg gggttcgagc cgccgacgac
     7981 gggggtgacg accggctcct ggtcgaagcc ctcacccagt gctctgcgga cgagcaggca
     8041 gtaggggtga tcgatggcgg tgcgcgccgg caggtactgc atccccttct cgatctcgag
     8101 accgcccatg cccatggcgg cggcatggcg gcggaacttc gccaggatgt cctcgggtgt
     8161 ctggttcttg accagccgca tgtcgatctt ggcggtggcc tcgcagggga tcacggtctt
     8221 ggtcccggcg ccctggaagc cggccgtgag cccggagatg ttgagcgtcg gccggaacat
     8281 gatcttctcc cagtggtcga gcccggcggg gccgaacggg cgggagaggc cgagcatccg
     8341 ttcgaacgcg gcctggtcgt gcggcagccg cgccatggcc tcgcggtcgg cggcggtggg
     8401 tggcacgacg tcgtcgtaga agccggggat ttggacgaca ccgttttcgt cgacgagcgt
     8461 ggcgaggaag gccacgagct ggttggcggc attgggcacg acgccgccat aagtgcccga
     8521 atgggtgtcg cgggcgccgg atcgatgacg aatggtgatg aagcagtcgc cgcgattgcc
     8581 gtgctggatc atgatccgat tgccgacgaa ggcctcgccg tcggcgtaga agacgtagtc
     8641 ggcggcgagc ttcgcccggt tgcgctcgac gaaggcggcg aggtgcggcg agccgacctc
     8701 ctcttgcgaa tcgaggacga ggatcagatt cgccaccggg ccggcgccga gccgatcgat
     8761 catctccacg gccttgaggt gggccaggaa ctggcccttg tcgtcggcgg tgccgcgccc
     8821 gtagagatag ccttcccgct cggtcggatc gaagggccgg ttggtccagg cgctctccgg
     8881 gtcgaccggc tgcacgtcaa aatgcccgta gatcaggagc gtcggcaggg cgtcgtcccg
     8941 gtgccggcga gcgagcacga tgggggccga gcccgggccg ttcacgtcct cgatcgcggt
     9001 ctcgaagccg agagcatcca ggcggtcgcg cagatagctg gcgcagtcgc cgagggcggc
     9061 gaggtcggtg ctcaggctgg gcaggcggca gagctccttg agctcctcga catagcgctc
     9121 gaagtggcgg tcgatatcgg cttcgtagcc gccgcgtgcc tcccgccacg ccgcctcgat
     9181 ctcgaccagt ccatccattt tgccatcctt cccttcgacg gctcagagcc gcttgaatcc
     9241 gtgtcgctcg acgatctcga ggatcaccct ttgggcgcgc tcgcgatgca accgataaag
     9301 gcgctcggcg ccgtccgcgt cgccggcgag gatcgcctcg accacggcac gatgctcgcg
     9361 cgtcgactcg accggcttcg gccgcagcct gagcgtgaac atccgtgccc ggtgggactg
     9421 ctcccaaaac cccatgatgg cggcacgcag ccggctgttg ccgctgagct cggccagaca
     9481 ccgatggaag cgctcgtcgg cgtgcgccca ggcgagacga tcgtcgatct cgagcgctgc
     9541 ttccatcgcc gcgcaggcat cgacgagtgg ctcgatctgg tccggcgtcg gccggcggct
     9601 ggcgaggagc gctaccgcct tgggctccag acaggccaga agctcgtaga tctcgcgggt
     9661 gtcgtcgggc gagagcgaca ggatctcgat gccgtggcgc ggcacgatcc gcaccagccc
     9721 ctcctgctcc agccgcatga tggcctcgcg gatcggggtg cgcgacagct tcagctcatt
     9781 ggcgatcgtg ccttggagaa cccgcgcgtt gggcgggtag tcgttgttga ggatctttcc
     9841 cttcaggtgg gcgagcgcga tctcgaaggc cggccggccg tttcggcggc ccgtggcgtc
     9901 gatatcgccg atgcctttca tcggtggtgg caggcgagca ggcggccgtc gtcgcggcgg
     9961 gcgagcggcg gctcggcctc ggcgcagagc ggcgtcgcca aggggcagcg cgtgcggaag
    10021 cgacagccgc tcggcggcga ggcggcgctc ggcacgtcgc cttcgagccg cggccgctcc
    10081 ggccgtgtgc ccgccgcctc gatcaccggg gcggcggcga gcagcgcctc ggtgtagggg
    10141 tggcgcggcg catcgaagag cgcggcggtg tcggcaagct cgacgagctt gccgagatac
    10201 atgacggcga cgcggtggct gatgctctcg acgatcccga gatcgtggga gatgaagacg
    10261 tagccgacac cccttcgctc ctggatctcg atcaggaggt tgacgatctg cgccttgatc
    10321 gagacgtcga gcgcgctgac cgcctcgtcg cagacgatca tcaccggatc gctggccagg
    10381 gcccgggcga tgcagacccg ctgcttctgg ccgcccgaga actcgtgcgg gaacctgccg
    10441 gcatcggcgg cgccgagccc gaccgtcgtc agcaatgccg ccacccgccg ctcgacctcc
    10501 gccgcgtccg tcagtccggc gaggttgcgc agaggctcgg cgatttgctc gccgacccgc
    10561 atgtgcgacg tcagggcggc gctcggatcc tggaagatca tctgcaggcg ggcacggacc
    10621 gcgcgcaggc cacgcctgtc gacggtggcg agatcgatgc cctcgaacag gacctggcca
    10681 tcgtcgggca gatcgaggcg gacggcacag cggccggtcg tggatttgcc gcagccgctc
    10741 tcgccgacaa tcgccagggt ctcgccccgg gcgacgctga aggagacatc gtcgacggcg
    10801 tggatatggc cgctgacgcg cgagagcacg cccgagcgga tgggaaagcg gcggctgacg
    10861 tggcgcagct cgagcaaggg ctcgacgccg ctcacgacga caccgtctcg tgggcgcgca
    10921 ggcaggccac gcggtgcccc tcgccgaatg ggtcgagctg cggctgacgc tccgcgcagg
    10981 cgtcgatcgc ccaggcgcaa cgctgtcgaa agacacagcc gcggccgcgc ttgcccggcg
    11041 gcggcaccga gcccgggatg tcggcgagcg gcgcgccact gccgcgccgg ttgcggccga
    11101 gccggggcac cgaggcgatc agcccctggg tgtaggggtg gcggggtgcc tcgagcaccg
    11161 tgcgcacaga ggccgtctcc acgacccggc cggcatacat gacgatcacc cgatccgccg
    11221 tctcggcgac gatgccgaga tcgtgggtga tcagcacgac ggatgtgccc cgacgccgct
    11281 ggaggtcggc gatcagctcc atgatctgcg cctggacggt gacgtcgagg gccgtggtcg
    11341 gctcgtcggc gagcaggagc acgggatcgc aggcgagcgc catggcgatc atcacccgtt
    11401 gccgcatgcc gccggaaagc tcgtgcgggt actggcgaag ccgccgctcg ggatcggcga
    11461 tgcggacctg gcgcaggagc tccagagcgc ggggacgcgc ttcgccgcgc tcgacgacgc
    11521 gatggcgcag cagcacctcc tcgatctggc ggccgatgcg catcaccggg ttgagcgcgg
    11581 tcatcggctc ctggaagatc atggcgatgc gtcggccgcg cagatcctgc atcgccgcct
    11641 cgtcgagagt ggtgatgtcc tgcccctcga agacgatccg gcccgcaggc gtgctcgcct
    11701 gaggtcgcgg caggaggccc atgattgcga gtgcggttac actcttgccg cagccgctct
    11761 cgcccacgag ggccaggagc tcgcccgacc tgaccgcaaa ggtgagatcc tcgaccacgg
    11821 acaggcggct ctcggaactg ccgaaagcga tgccgaggtt ctccacctcg aggacgttgg
    11881 tcgattcctg gagggcggtc acggctcaga tcgccttggc gacgcgcgga tcgagggcat
    11941 cccgcagcgc gtcgcccatc aggttgatgc cgagcacggt cgcggcgatg aagcagcccg
    12001 ggatcagcac gctccagggc gcgccacgga aatagatgcg gttctcggcg atgatgttgc
    12061 cccagctcgg catgtcgggc ggcgtgccgg cgccgatgaa gctgagatag gcttcggtga
    12121 tgatcgccga ggtgcagatg aaggttgctt gcacggccag gggcccgagc gtcatcggca
    12181 ggagatggcg gaagacgatc cgcccggtgc ggttgccggc gatgcgggcg gcatcgacga
    12241 aggtctgctc gcgcagcgac agcaccatcg ccctgaccag gcgcacgacg cgcggcacat
    12301 cggcgatggt gatggcgacg atgacgttga cgagtccggc gccggcgacc gacatgagcg
    12361 cgatcgcgag caggatgccg ggtatcgcca tgatgccgtc catgatccgc atcaagacgg
    12421 cgtcgacgac ccggatgaag ccggcgagga ggccgaggac gaggccgatc gcggtgctga
    12481 gcacggccac ggcgatgccg atggccagcg atacccggcc gccccgcagg acgtgggcga
    12541 agaggtcgcg gccaaggttg tcggtgccga aagggtgcgc ccaggatggt gccgtcagac
    12601 ggatggcgcc gtccatgccg gccggcgcca agaccagggc gccgagcagg atgccgagga
    12661 gcggcaggct gaacagcacg agccccgggc ggaagcccgg tcgcgtgaag atcgcaccga
    12721 gcccgcggcg gacggcgcca tgccgggggc tgctgagcgc ggtagcgtcg gcggtcgcca
    12781 tggggtcggt cagtagcgga tgcgggggtc gacgagcgcg tagatcacgt cgaccgcgag
    12841 attgaggaac acgtagacga acgagaagaa aaggacgacg ccttgcacga ccggatagtc
    12901 tcgggtgaag atgccgtcga ccgtgagccg gccgatgccg ggaattgcga agacggtttc
    12961 ggtgatgacg acgcccgaga tcagggctgc cagactcagg ccgatcacgg tgacgatcgg
    13021 caccgcggca ttgcgcaggg cgtgccgcac caacacctgg aacgtccgca aacccttggc
    13081 gcgggctgtc cggacgaaat cctggcgaag catctcgagc acgctggccc gcgtcatgcg
    13141 ggcgagcagc gccgcatagg cgagacccaa agagagagcg ggcagcgtca agtattcgag
    13201 ccaggggccg atgccgcggg ccagtggctt gtagccctgc accgggaaca gcccgaggtc
    13261 gagggcgaag acgaggatca gggcatagga gatcaggaag acgggaaacg agtaggccga
    13321 gaccgcaaag accatcactg cccgatcgat caggctgccg gccttccagg cggcgatcgt
    13381 gccgagtggc acggcgatgg cgacggcgat cgtcatggcg tagagggcga gactcagggt
    13441 agcgccgatc cgctggccga tcagcgtcgt caccggcatc tgggtgtaga tcgagacacc
    13501 gagatcgccc tgtagaagca ggccgaacca cttgacgagc tggaccagga tcggcgcgtc
    13561 gaagccgtag cgggcgcgga tcgccgcgat ctgcgcgtcg gtcgccagct cgccggccag
    13621 gacggcggcc gggtcgctgg cgccgaggcg catgatcagg aagacgacgg tggccaccac
    13681 caacatcacc gggatggtga gcagcatccg gcgaacgatg aaagcggcca cgacgtgcgc
    13741 cccctgctac tcttcgatgc tgatgcccca gagatgggcg accgatgcct cgcccacacc
    13801 cttcaggcgg ttgttccagg cgtcgacctt gtagaagacg ccgagcggaa cgtgggtgcc
    13861 gagctcgaag gcgaggcgct ggacctcggt ggcgatccgc tgcttctcgt cggcgtcctt
    13921 ggcggcggcg aagtcctgcc tgagctgctg gatctcctcg ctgcacggcc agcccggcca
    13981 gttgtcctgc gcgcaggagg cgttgatcgc cggattcacg agcggattga agacgtcgat
    14041 ggtggcgaag gtcgtgtgga acaggttcca tccgccctcg tcgacgggcg tcttgatggc
    14101 gcggcgctga tgcagcgtcg cgacggcgat gccctgaagc tccacctcga gcccgatctg
    14161 gcgaaatgtc tccgcggtcc tgatgttcgc gccgtgcagc agcggatggt tggtcgtgtc
    14221 gaggaagacg atgggctggc cggcatagct cgactccgcc acgagcgcct tggccttctc
    14281 caggtcctgt gcctgcagcg gctcggagcc gacctcggtc gagtactccg tgccgcagcc
    14341 atacatcgag tagcaggtct tccagagctc gggatcgggg atcagcgagc ccatgtactc
    14401 gtcctgggtc accgcccagt agatggcctg gcgcagcttc tggtcgtcga acggcgggac
    14461 gagatggttg aagcgcagcc agagctgctc gccgcggatg tcgcgcgtct cgaccacgag
    14521 atcgggatcg gcgcgcagct cgtcgacgag gtcgtgcgcc atgttgccga gatagtcgac
    14581 ctcgtcgttg aggagcgctg cctgccgtgt cgcgtcgtcc ggcatgatcg tgtactcgat
    14641 cacgtcgacg ccgggcacct tggcgccggc gaggttgtcc tggggatcct cgcgcggcac
    14701 atagctctcg ttgcggcggt agatgcgcgt ctgcccggga acccactcgg cgcggtcgaa
    14761 ggtgaacggc ccggagccga tcgattccag cacctgctcc gtgttgggca actgggcgaa
    14821 acgctcgggc atgatgaaga ggacgttgcc gctcggcttc gccatggcgt cgatgatcag
    14881 cgtcgacggg ctcttcatcg tcatcacgaa gctgtcgccg ccggtggcgg tgatgtcgtc
    14941 gacataggtg atcgcgatct gcgccatggt gtcggccttg gcccagcgct ggatcgaggc
    15001 gatcacgtcg gcgctggtga caggagcgcc gtcgtggaag gcgagacccg gccggagctt
    15061 gaactcgatc gtcatgccat cgtcggcgac ggtgtagctc tcgagcatct gcggcttgat
    15121 ctcgaaattg gaatcgaccg cgagcagggt gtcgtagacg agataggcgt ggtcgcgtgt
    15181 cggatgcgcg gtcgtgaaca tcggatcgac cacctggacg tcggtcgagg tgccgacacg
    15241 gagcacggtt tcggcgttgg cgttggccag cggtcccagc gacccgaaag cgatgatcgt
    15301 cgtcgccaga agccgccgcg tcgaagcgtt cattcttgtc ctcccgcctg atactgcaca
    15361 gggtcagtca ccttgcatca cttgacgtat ccgtcgacgt atccattgtc aaacccgttg
    15421 tgaggttgat cgaccgatga ggcgcccatg agccacggat cggagacctt cctcggatcg
    15481 aactcgcgcc tgaatctcgg cgggcagatc gtcgaggagc tcggacagcg cattgccagc
    15541 ggctactacg gcgcggccgg cgtgctgccg aacgaagcgg cgttgagcga cgagctctcg
    15601 gtcagaagct cgattatccg tgaggcggcc aagcagctct cggcgaaggg gctggtggag
    15661 atcagatcga ggaccggcac acgggtcctg ccgcaggacc gctggaacct cctcgacata
    15721 tcctcggcgg ccaaccagca ggcatggccc gagagccggc gagacggcgc caagaggccg
    15781 aaacgggtac gcgactgacc ctggtctgtc ctcttggcct ttttgtcgtg cggtctgtgt
    15841 tgcgtcaggc tgcaggcaag agaggatctc gatggcagaa acggcgaccc gcaagcgggt
    15901 gagcgcgaag gcaccgacgg tgaagctgat acaccgtcgg ctgacggttt tggggttggc
    15961 agagacgttg gggaacgtgt cggaggtctg ccagcgtggg gcaatcgctc gacggacgcg
    16021 ctcatcgact tctacctcta tggcgtcacc cgtttcggca ggcagcaagc ggagcgatag
    16081 gagcgtgagc ttcgccacgc gatctccctc gccgccgaaa acacgcgcat ggcgaccgag
    16141 cgggcggcgt gccggccacc ggttcgaatt cacgaccacg gcaagcatta catcgtctat
    16201 ctgatccgtg acgatcacgc gctgatcgtt cgcgtcttgc gagacggggt gaacctgagg
    16261 cggcatctgt aggcgccgca ttctctgtcg atcgcctttc aggtggcgcc cgaccgcccc
    16321 ggcatccgtc agccgcccgt ggcgaagccc gggtagcagg tcatgccgcc gtcgacgaag
    16381 agcgtcaccc cgttgatgta gtcggccatg tcggacgcga ggaagaccgc cacctgggcg
    16441 atgtcctcgg gctcgccgat ccgcccgtag ggcacgagct tcatcagctc ggcataggcc
    16501 ttcggcgtgt cccaggcctt ggtgttgatc ggcgtgcgga tggcacccgg gccgatcgcg
    16561 ttgacccgca cgcgatgcgg ggccagctcc tgcgccaggc tcttcatcat catctggatg
    16621 ccgcccttgg atgccgcgta gttgacgtgg ccggcccagg ggatgacctc gtgcaccgag
    16681 ctcatgcaga cgatcttgcc ggccgccacc gagacctccg gccgcacgcc gcgccgcctg
    16741 aactccttca ccgcctcccg gatgcagagg aactggccgg tcaggttgac gccgatcacc
    16801 gtctcccact gcgccagcgt catctccgtg agcgccgcgt cgcgctgcag gcccgcattg
    16861 ggcaccagga tgtcgagcgt gccgaaggcc tcgatcgccg cctggaacat cgcttcgacc
    16921 tcggcctcct tcgagacgtc cgcctcgagc gccaggcccc gcaccccctc ggcctcgatc
    16981 tgccgcacca cctcccgggc cgccggctcg ttcgtcacgt agttgacgac gacatcggcc
    17041 ccggcccgcg ccaggccgat cgcgaccgcc cggccgatcc ccgaattggc gccggtgacc
    17101 agcgccttct gcccgaccag cagcttcggg cagcgcccgt aatccggcag ccggggctcc
    17161 tcccggccgg tcgctcgctc cttcgccatc gtctctccct ccctgatccg ccgccaaccg
    17221 ttcgcgccga ggctaacagc tcgaagccat gcgcgtcgac cgcggctgga acggtgttgg
    17281 cgggcggcct gcggcgccgc accgtcaaaa acgcccggcc ggcggcgacc ggccgcggga
    17341 ggaaagatcc cccccgcgct tcgcgccctc gccgtgctcc tgcccctcgc ggccgccgcc
    17401 cgctcgcgca tcgccgcgtc gtgatctgtt gcggggtcag ccggtcgtcg tcgggcgggc
    17461 ctcgcgcgag ggctcctggt cccggagccg gcctttcaag tgctcggcga ggcggacggc
    17521 gagggccacg atggtgaggg tcgggttggc gaagccgctc gtcgggaaga ccgagctgcc
    17581 ggcgacatag agattgcgca cgccatgcac ccggcagtcc gcgtcgacga cgcccgcgga
    17641 cggcgacgca tgcatgcgcg tcgtacccat gtgatgcttg ccgccgaacg ccgcggcgcg
    17701 ccaggcttcc gccgtgtcgt cgaaggcgag cgccagccgg ccgtgcccgg cgccggctag
    17761 ggcctggtcg tgcaacgcga gcacccggcg catgctggcg aggtcgcgct cggagaggcg
    17821 ccattccacc cgcggcaagg gtcggtccag cgcgtctttc ccggcggcga ggcggacgcg
    17881 gttgtggtgc agcgattcgg cctcgaagaa ggcgcgcagg cgccagctcg aggtcctgcc
    17941 ggggcacacg gcgatgcggc gccaggcggc gtggacgagc cggtgcggtg cccggagcgc
    18001 ccggcggaca gggtcgccgt tcccgcccgg cacgccacgg ccgcgcacct tctcccacag
    18061 ctccagcatc gccttgacgg cggggtcggt gaacacggga tgcgcctcgt aggccggcag
    18121 gaagaagacg gcggcgttca gcaggccctc gcgttccagc gccgggcgcc cgagcgacaa
    18181 cgcggcgcgc acattggcgt cgggcggcat gtcgtcggtg cgcgccggga aatagaaccg
    18241 gagcgagatg gccggatcgc gcggcaggaa ggagccgggc tccatgaagg gatgctcggt
    18301 gaagtagcgg ccgacgagcc cccgggcatt gccgatgctg gtttcgggtg ttggacccga
    18361 gagcagcagg agccgggcgt tctcgatgcc gcccgtggcg agcacgaaga ggcgcgcggc
    18421 cacggcgaag cgccggccat cgagggcgcc gacctcgacc cgctcgagcc ggtcgctcgt
    18481 cggtgccgcc cggcaccgca gcgccacggc ccccagcagc acccgtatgt tcggtgtggc
    18541 ggcgagggcg gcacgatagc ggctgccgaa gcgggtcggg ctgacctgga acatcgtcgt
    18601 gacgacgtcg tcgccggcca gcttcaggtg ctcggcaccg aaccgcttcg cccaggccgg
    18661 cgggtcgtaa tccacctcgc cgaggccgca gatcgcatgc gcacgctgat aatgcggacg
    18721 cagggagtcg tgcccgaatg gccagccgct gttcggcacc caggagcgct cggtgaaatc
    18781 gatcgggtcg agcggccggc accagccgcc ccagatccgg gtggccccgc cgagaccggc
    18841 aaagcgggtc tccttgagcg gtggatagga atcgccgacc acctcgccat cgaggagcgc
    18901 ctgggctcgc cgccggtagg cgaggccgcc ggcatcgagc aggcagatgc gggcaccggt
    18961 cgcctcgagc tcgagcgcca cggtgatgcc ggcggccccg gcgccgacga tgcagacatc
    19021 gcagtcgatg cggctgcgat cggcgacgcc agccacctcc tcgatcacgg caaggcgggt
    19081 ccaggggaca ggtcgatcgg ccggccgtcc caggccgggt ccgcctcgac ccccagccgc
    19141 gccatcaccg tcggcacgag atcgaggatg ctgtggccgg gctcgagggc acccggtgct
    19201 atcccgggtc cggccgcgat cagccatcca cggtcacgat ggttgccgga gcggccggaa
    19261 ggcaggcgct gtggcacgtc ataggcgaag ccgggcacct ggtcgcagac gacgcgacgc
    19321 acctggcgtg ccggcaaacc ggcccaggtg acgaccagat cgggcagcac gtcgcggcca
    19381 tctgcggtcg ccggtgcttc ctgccaggcg cggtgcacgg cagcggccag cgccgcaccg
    19441 ctcccggcgt cgcgcaacgc caggatgagg ccctcgagcc ggtcgcagag ggccgtgtag
    19501 gccgcgccgg gctcgacgat cccgtcgcgc tcgcggcccg cgacgttgag gcgcaagtag
    19561 ccggcgtggt ccatggccag cgggaaatgc cgggtcgtcc gccagtcgta catgccagac
    19621 gaccagagct cgaccagccg gccggtgacg gcgggcggca gccgggtcag gatcgggcgc
    19681 gcccagtgga agggaatgcg ccgcttgagg ctgtagagca ggccgcgctt cggtggccgg
    19741 ttgtcggccg cctgcagggc agcgtcgagg atgtcggcga acagatcggc ccagccggga
    19801 ttggggccca tgccgtgaac ggcgaaggcg agcaccgtgg tcgcgtcgtc gacgcgcgcc
    19861 acgatctcgc cgaccgtccg gtcgaccgcc acgtagagct cggccagggc cccctcgagc
    19921 gccgtccgtc tggccgcgtc gagcggcgcc tggtcgattt cgcccaggtc ccacagatag
    19981 tggccgccgc ggtgtgtggc accgagtacg acgcaggcca gatcccactg ctcgcggccg
    20041 agaagatcca ggaggatctg ctgctgctgc gccgtggcgt ccagcagcga gtcgcgcagg
    20101 cgggtcaggc tggacgcatc ctgggggccg aaatgctcgg gcggcatcgc cggggcgcca
    20161 tggcggttct cgagctcccg ccagagctcc ggtggccacg aacccctggc gatcacgtcg
    20221 tgcgtgcccc agccgccgag atagaggccg tcgagcggcc gcggcggacc cagcaccatg
    20281 ggcacgtcga cgatgcaggc ccgcaggccc cgggcggcgg cgtgctccca gaacggccgg
    20341 ctgccgagcc acgcatcggt cggcacctcg atccgcatcg tcgaggctcg ccagagcttg
    20401 ttgtggtaga tgccgtgccg ggcgaccggt cggctcgcat agaaggtcgg ccagacgccg
    20461 ccggcataat cggcggccgg cgaggtgagc ggcccgaacc ggccacgccg cgcgagctcg
    20521 gccaatgcgg ggagccggcc ttcggcgatc catcgctcga tcagggtgag ctcggcggcg
    20581 tcgaggccga gcatgaggac cctctggtcg gttcggggct gcgtcatgag gccattcaac
    20641 gacaaagcgg cgcgccatgc accagttggg ttcgactgcc tgaagctgtt ggacgaagat
    20701 gccacggcag acgggatcgc ggcaccacca tcgcgtcaat gacaggtcgc cggtcgccgg
    20761 gaaagcgcta tcccggaacc cggacaagcg caacagcctg ccgcgtcccc ggcctctgca
    20821 ctccacccgt ccagccgggc cacgccgggt ttgccaaggg cgatgccggc ggactacgtt
    20881 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctcgtag aggcgcaccg
    21001 gcgggggtga ttcgagcagc ggcaccgcca cctgccacag ccgctcgatc ccctccgcgg
    21061 tggtgaagag cgtgtggtcg ccgcgcatgg cgtcgaggat cagccgctca taggcctcca
    21121 gcacgtcgcc gatcagcccg gtctcgtgca tggcgaactg catgctcacc ttgtccagcc
    21181 gcatgcccgg gccgggtcgc ttgccgtaga aggagagcga cagcttcgag gaatcggcga
    21241 ggtcgaaggt gaggtggtcc gggccctgca cgccgacccc ggagccgggc gggaacatgc
    21301 tcttgggcgg ctcgcgaaag gcgatggaga tgatcctttg gccctcggcc aggcgcttgc
    21361 cggtgcgcag gaagaacggc acgccggccc agcgccagtt gtcgatgccg cacttgagcg
    21421 cgatgaaggt ctcggtgtcg gaatcgggat cgacgccatc ctcgtcgcgg tagcccacat
    21481 actggccgcg caccacgtgg cgcgggtcga gcggcagcat gctgcggaag accttgttct
    21541 tttcctcgct gatcggtttc ggctcgagcg ccgtcggcgg ctccatcgcc atgaaggcga
    21601 ggatctggaa gaggtgggtc accaccatgt cgcggaaggc gccggtctgc tcgtagaacc
    21661 cggcgcgcat ggcgaggccc agcgtctccg gcacgtcgat ctgcacgtgg tcgatgaagt
    21721 tgcggttcca gatcggctcg aagagcccgt tggcgaagcg aaaggcgagg atgttctgcg
    21781 ccgcctcctt gccgaggaag tggtcgatgc ggaagatctg ctcctcggtg aagtgctcgt
    21841 gcagctgccc gttgagtgag acggcactct cgacatcgat gccgaagggc ttctccatga
    21901 tgatccggca gcttttcgtc agctccgcct cggccagcag ccgcaccgcc ggcagggcgg
    21961 cgctcggcgg cacgctgaga tagtgcatgc gccgggattc gcccccgaga ccgcgctccg
    22021 ccgcctgcac cgccttcttc agggcaccgg cgcccgccga catgggcacg tagtcgaggc
    22081 atggggcgaa ggccgaccag tccgcctccg cgaccgggcg cgggtagaac ttggccagcg
    22141 cgtcgcgggc gatggtgcga aaggcgtcgg cgtcaatctc gtccagcgac acgccgatga
    22201 tccggcagcc ggggatgaag ccctcgctgc acagatgaaa gagcccggtc agcagcttgc
    22261 gccgcgacag gtcgccggtg gcgccgacga ggacgaccac ctgcgggtat ctcggcccga
    22321 ccctcgagga attcttcgcc aggatccgat cctcgcatcg ctgtcaccgg ggacgtaccg
    22381 ccatactgga acctcgggcc cggcccgcgc aatgaaattt attgcaccgc acaatccggc
    22441 ggcgccgtca tttgtcgcgg ataccgcgac agccgggccc gggttgtcgc gttatccgcg
    22501 acattctcgc ttgggttgtc gcggattctg cgacatgccg cggccgatgt cgcggaatct
    22561 gcatcatgat tatgaccaat gtcgcggaat ccgcgacaga taacgacgaa cgaacctgcc
    22621 gaatcgacga atgaacccac ggattcgacg agcgaacccg gggatttgac gaacgaaccc
    22681 gaagcatgac caacgaaccc gaaggccgcc ggccgctggc ggtgcggctt tctccgccgt
    22741 ccggggtgcc ggcacccgcc gaaaaggcga ttttcccgaa cgaacccgtg atctaggaca
    22801 atcgtccttg atagcccggc ctgccgcgtg tatgatatgg gtcggtcgta cgaggttcgg
    22861 ccagggaagc gacgcgtggc gaaagcgaag gaacggctcg accagctcct ggtggcccag
    22921 ggcctggcgc cgacccgggc gaaggcgcag gcgctgatcc tcgcgggcga ggtgttccgc
    22981 ggcgaggagc gcctcgacaa gcccggcacc tccctcgatc cggcgaccga gctcaggctc
    23041 aagacaacca gtccaaccta tgtcagccgc ggcgccttca agctgatcgc cggtctcgac
    23101 cgcttcgcca tcgacccggc aggcctgctc tgcctcgata tcggcgcctc gaccggcggc
    23161 ttcaccgagg tgctcctgga gcgcggtgcc gcccgggtgg tcgccgtcga tgtcggccac
    23221 ggccggctcg actggcggct gcgcaacgac ccgcgcgtgg tggtcctgga aaagaccaat
    23281 gcccgccatc tcacggccga tctcctgccg ggggcgccgt ccctgatcgt ctgcgatgcg
    23341 agcttcatcg gcctgcgcac cgtcctgccg gcggccctgg gtctggcggc gccgggggcg
    23401 tggctcgtgg ccctgatcaa gccgcagttc gaggcgggca gggcggccgt gggcaagggc
    23461 ggcgtggtgc gcgacgccgc gatccacgag gccgtctgca ccgatgtgcg ggactggctg
    23521 gcgacgcagg caggctggac cgtgctcggc atcgagccga gcccgatcct cggccccaag
    23581 ggcaatcgcg aatttctgat cgccgcacgc cgcccggccg ctcggaattc ttgagccccg
    23641 cccgctgccg gggcacgctc cgccgatggc cgaaccacca accaccctgc cgcccttcgc
    23701 cgctctccgc gccttccagg ccgcggcgat ccacggccgg ttcaaggacg cggcggtcag
    23761 tctcggcgtc accgaatcct cgatcagcca ccagatccgg cgcctcgagg acttcctgca
    23821 tgtcgccctc ttcgaccgct ccgccgccgg cgtgcagctc accgcggccg ggcgccgcta
    23881 cttcgccgag atcgacccgg caatccgccg catccaggcg gcgacatccg gcctgctgcg
    23941 cccgtccggc cgttctgtcg tccgcctgac tctgccgccc tcgctcgcgg tgagctggct
    24001 gatcccgcag atctcggcct tcgagagcga gcatccgggc atcgagctgc agctcgtcgc
    24061 caccacgcgg gtgatcgacc tcgcccgcga ccaggtcgac ctcgccatcc gccacggccg
    24121 cggcgcctgg cccggcgtcg aggccgagct tctgctcgac gagcgggccc tgccggtggc
    24181 cgcacccggc tatctcgagc ccgcgcccgg cgagacgccg agcgaggcgc tgggccgggt
    24241 gcggctgatc gtcagcgccc gcttccccaa cgaatgggag gagtgggcca gggcacgcgg
    24301 cctgccgccg ccatcgcagc agggtgccat cggcctcgac ggcatcgagc aggcgctgca
    24361 ggtggcggaa agcggccacg gtgtcgccat gggccgtcgg ccggtggtcg acgagcatct
    24421 cgcccgcggt gcgctcgtgg cccccttcgg cgagggcgac ctcacgggcg ccgcctattt
    24481 cctctgccgc ccgccggata tcgaggtcac gtccgccgtc cgccaggtgc agcgctggct
    24541 gcacggtctc gcccggcgga catgaacggg cttcaagtcc ggctgaagcg aactcgttgg
    24601 tatcccccgg ctcgacgcgc cagcttcggg tggtcaagcg atggccgacg agaccggcca
    24661 tccggcccga ggagtgaagc catgaccatc gtcctgaaca aatctggcat cctgagcgaa
    24721 tccggcagcc tcgccgctcc ggcccggcac ccggcaaacg tcacggccga gctgaccttc
    24781 atcagcccgt cggccgcgcg ccccgtgctg atcagctccg ccgtcaccgg cggcgccccg
    24841 gacgagcggt tcggcaccga gcgccatgcc gtccgcgtcg aggacatgcg ctcgctcgcc
    24901 ggcgccctgt cgctcgatca cgagggcttc gagctgcatc gtgcacccac ggcggtcgcc
    24961 gacctctacg acgatgcggc ggtcgagcgg acctaccatg cggagaccga ggccttgctg
    25021 cgtcgggtga ccggcgcctc gcgcgtcgtc atcttcgatg ccacccgccg ctccgatgcc
    25081 gccggcggtg ccgccaatcg cgacggcagt cgtggtccag ccggccgggt gcatgtcgac
    25141 tacacggccc gcagcgggcc gaagcgcctg gccgacctcg tcggtgccga cgaggcgcga
    25201 cggctcgccg cgacgggcgt gcgcgctgcc cagatcaatg tctggcggcc catccggggc
    25261 ccggtgcgcc ggtcgcctct ggcgctggcc gacgcctcga cggtccggcc ccagcatctc
    25321 gtcgccaccg accagatctt ccccgaccgg gtcggcgaga tctaccacct gcgccatgat
    25381 ccctcgcagc gctggtacca cgcgccgctg atgaccgcgg acgaggtgct gctgatcaag
    25441 ggctgggaca gcctggagga tggccgcgcc cggttcacgc cgcactccgc cttcgcgctg
    25501 gctgacacca ccgccgcgac gccggcccgc gagagcatcg agctgcgcgc ctacgtcatc
    25561 ttcgcctgat ccatcccctg cgcgccgtac cagggcgccg tcagctgctg gcggcgccca
    25621 gccggacgaa gccggcctcg aggtcggcaa tcaggtcgtc ggggctctcg agcccgacat
    25681 ggatgcgcat catccggcct tcatgcggcc agtcggtcgc cgtccgcagc ggtgctggat
    25741 tggtcgggat gagcaggctc tcgtagccgc cccagctata acccatgccg aagagctcaa
    25801 gatggtcgag cagggcggcg aggcccggcc gctccaccgg ctgcagcagg aagccgaaca
    25861 ggcccgaggc acccgagaag tcgcgccgcc agagcgcgtg gcccggatcg tcaggcaggg
    25921 cagggtagaa cacgcgcgcc acctcgggcc ggccggcgag ccattccgcc accttcaggg
    25981 cactcttctg gtgccgctcc agccgggtcg ccagcgtccg caggcccctg agcgccagat
    26041 agcagtcctc cggcccgggg gcgccgccga gctccgcata ggccatgcgg atgccgggct
    26101 cgagctcggc cgtgcaggtg atcagcccca tcatca
//
LOCUS       DAOTFI010000026        25758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2196_length_25758_cov_7.696533, whole genome shotgun sequence.
ACCESSION   DAOTFI010000026 DAOTFI010000000
VERSION     DAOTFI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 25758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25758
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2196_length_25758_cov_7.696533"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..557)
                     /locus_tag="P5558_04085"
     CDS             complement(51..557)
                     /locus_tag="P5558_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRY23521.1"
                     /translation="MHRAFAALAVVPLVALGFLAIGPVAAAADQGLPEPVIDSDESEL
                     FALNLYHEARSEGRDGMIAVGWVVLNRAADPEYPDSVEGVITEGADSRCKWGWLCDGK
                     SDQPKEADMWALARDVTELMASPDRPADPTRGALWFHRTVREKPSWMADQVQRTATLG
                     SHHFYGRN"
     gene            1163..2974
                     /locus_tag="P5558_04090"
     CDS             1163..2974
                     /locus_tag="P5558_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HRY23522.1"
                     /translation="MNDVTPTANERLPFRAGDFVTLAEGLDYAARGETGVNFFSTRGS
                     LETALPYSELRERAIALARRLVGTGLRRGDRIGIIAETSPDFLAVFFACQYAGFVPVP
                     LPLSINFGGREAYVERLRGMLAAAGARAAVAGPELVQVLAAATEATIVRFTATADELM
                     ALPVSDQPLEALGADDACYIQYSSGSTSFPRGVLVTQRALAANARSIATDGLALGPTD
                     RCTSWLPLYHDMGLVGCCLTPVMCQISVDYLATTAFARRPLVWLQLLAKHGGTISFAP
                     TFGYELCARRAANGAARDLDLSRWRVAGIGGEMIRSRALTAFADTFGACGFDARAFVP
                     SYGMAEATLAVTFNPLGRGVRIDRVARGETLERQRLALPAGEAVPPSESRSFVVCGAP
                     MPGYAIEIRDDSGRVLPERHVGRICIQGPSLMEGYFRNPEATAAVLRGDGWLDTGDMG
                     YLVGGELVVSGRTKDLIIVGGRNIWPQDIEWAVEKLDAVRAGDVAAFAVTDSLDRERV
                     VVVVECRQTRPAEQAELRHEVAAVVNRLAGIECEVVLAAPRSLTFTTSGKLSRAAARD
                     HYQRGVLRDLDAAAADDAFDGAVEPGQPAVTALAEAS"
     gene            3064..4017
                     /locus_tag="P5558_04095"
     CDS             3064..4017
                     /locus_tag="P5558_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY23523.1"
                     /translation="MTRTVALTGATGFVGRHLVARLGQAGVGVRALSRRGPIEGAVTV
                     AGDLADRAALDELVRGAWRVLHLAGVIKTPRPAEFTAVNVEGTRHVAEATAQAGLKLT
                     LVSSLAARHPEVSAYAASKRAAEETARAVMPEGDLTIVRPPAVWGPGDRATLVVFRQL
                     LAGWLVVPAPKGARFSLLHVADLVGLLVDHVLDRLALPPLAEPDDGTPGGYGWDELAR
                     TAERLTGRRVRLLRPPTGLLAGPARLADLIARATGADLPLSADKLGELAHADWVAAGP
                     ALPWRASRITFDQGFAATVDWYRAAGWLPATATRTADGSKA"
     gene            4014..4256
                     /locus_tag="P5558_04100"
     CDS             4014..4256
                     /locus_tag="P5558_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY23524.1"
                     /translation="MTENVLNEIMALMQRYNPDGKPLDAGTELGADLNIDSVAAMDLI
                     MEIEDKFDIDIPMNQVAELRTIGDLVALAEGQLPKG"
     gene            4297..5505
                     /locus_tag="P5558_04105"
     CDS             4297..5505
                     /locus_tag="P5558_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY23525.1"
                     /translation="MDIFDKYAGIEQRHRALLSIGGDPFAVRVDRIISATEAMIGGRR
                     TILVGTNNYLGLTFDPACIEAGREVLATDGTGTTGSRVANGTYGRHTDLEAAFARFFD
                     RQKAIVFTTGYQANLATICGLAGPDDVILADADSHASIWDGCKLSPAETVVFRHNDPD
                     HLDKRLTRLADKGQCKLVVVEGLYSMLGDTAPIKEFVEVKKKHGAFLLVDEAHSFGVF
                     GDKGRGVAEAQGVEADVDFVVGTFSKSLGGSGGFAVSDHPKFDLLRLASRPYMFTASP
                     SPATMATVSEALRQIEARPELRTRIWANAEQLHTGLRDLGFTLLAPLSPVIAIRMPDE
                     ATAVMAWNRLIEAGVYVNLALPGGTPGGVCLLRCSVSAAHEPAQIATVLGCFAELASE
                     LDRLKGQNAA"
     gene            5734..6891
                     /locus_tag="P5558_04110"
     CDS             5734..6891
                     /locus_tag="P5558_04110"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRY23526.1"
                     /translation="MTRVAIRPELLRWARERAGIHDVGDLISRFPKLGDWETGEAQPT
                     LKQLEAFAQTLHVPIGYLFLSEPPVERLPIPDFRTHGGRGVRSTSPDLLDMLYACQER
                     QGWYRDFALTVRMSEAAFVGSADLGERPQDVAARMAETLGFDVAARAACRTWEEALRL
                     FIAQADAAGVLVMVSGVVLSNNRRILDPEEFRGFALADKRAPLVFINGADTKSGQMFT
                     LAHELAHLWLGSSAISDASAAPLYGYRREEVWCNAVAAELLVPLAVLRPAIERDEPLD
                     RAMQRLARQFKVSTLVILRRLLDAGALDRPAFDRAWAEERTRLRELARSAGGGGDFYR
                     TTLSRVSRRFARALVESTLEGQTLYRDAFRMLGVKKTETFNNLGREVGVLG"
     gene            6888..7394
                     /locus_tag="P5558_04115"
     CDS             6888..7394
                     /locus_tag="P5558_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HRY23527.1"
                     /translation="MTRHLLDTNIFIQAKNLHYGFDFCPAFWEWLIERNVAGCVASID
                     KVGDELQAGGDDLSDWATARGRAFFLPPDDPVLPALGRVSAWATSRNYEAAAIATFFQ
                     VADYWLVAHALAHDFVVVTHEVPSDSTRKIKIPNACIGLGLTCISPYEMLRRERARFV
                     LGPAPRVA"
     gene            7502..7633
                     /locus_tag="P5558_04120"
     CDS             7502..7633
                     /locus_tag="P5558_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23528.1"
                     /translation="MPCVALLRCSVSAAHEPAQIATVLGCFAELASELDRLSKQDAA"
     gene            complement(7644..8531)
                     /locus_tag="P5558_04125"
     CDS             complement(7644..8531)
                     /locus_tag="P5558_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23529.1"
                     /translation="MAAARGDLEAVLRAELATPAPEAVQAIARAACERHAPGIAAVLF
                     YGSCFRDGVVMDRLVDLYLLADDYARTGQGRLAARANRLVPPNVFYLEIEHAGETVRA
                     KYAIVTLDQLEAKVSPETDNPYFWARFAQPTGLVWCRNDATRRRVTNILAEAVRTTFA
                     AARALTRDRAASEALWTVVFLETYRTELRAEKPDRAAEIYQSFAARYDRLTRALIGEN
                     VRPRHRSWARTRRVGKALSILRLMKAAFTFSGGADYLAWKIERHSGQPVPLTDWQRRH
                     PILAAPPILWRLWRSKAVR"
     gene            complement(8534..9490)
                     /locus_tag="P5558_04130"
     CDS             complement(8534..9490)
                     /locus_tag="P5558_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRY23530.1"
                     /translation="MRIGLLSNPGSTRNRVGMAAIEALAERTPEILHRRFEPALGFGP
                     PLQSFAAAGCDLVVVSGGDGTVQGILTELLAHHPFAELPALAILPRGMANMTATDCGL
                     AGHDAATLERLVAACRAGRLADSLVTRHILKADFADGVPAPRGMFMGAAGIIDIILFV
                     TGRLHSRGIKGEWSHAATILGLLGLCLFRGFQALGLRAHRIGLAIDGTARRDEVLAIL
                     IATTLDRLVLRSRPYWGAGDLPLRHTRFAHPPRGLVRHAWRLLYGGADRRLPSDSYRS
                     GGGRRLELWLDGPFTIDGEFFTPAPGRPVTVTADETIRFARV"
     gene            complement(9490..10575)
                     /locus_tag="P5558_04135"
     CDS             complement(9490..10575)
                     /locus_tag="P5558_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRY23531.1"
                     /translation="MSVLTAYLNRMVLLRVTAAVIAISLFALVFDLLDASDDIIRTEG
                     NVALAFGRYFALRLPSLVAEVLPFAALLGALFAAAELLRNSEFIVLWASGVSPLGIVR
                     RLLPIGLAICALELANDELLVPPTIQELRAWEVGSFKSTLDGFVGNDIWMQHRGDFVR
                     LPRLEPGATSASNVIILERDSVGNLVERLTAARAELLPGAWRLLDVQRATVGAGFTTE
                     PELVWENEIDLDRLRVVARPPQEIGLLDLVDIIRNDGYGVVATQGHESALFHRLFGAT
                     LPTLLVMLTFALARRSTRQGAISTLFMKGIGGGFVFVILNGLALALAEAGFLPPLAAT
                     AGPMLLLLALVVGLPLRDELLLRRRAA"
     gene            complement(10572..11795)
                     /locus_tag="P5558_04140"
     CDS             complement(10572..11795)
                     /locus_tag="P5558_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRY23532.1"
                     /translation="MLGTLDRYVLGQVLRPMLAAILITLLALLAERALRVVDLVIGWR
                     GSLAIVFEMLSYLVPHYMVVALPAAFFLGIMLAIGRLSREGELDAMRAAGIGLARIAR
                     PLVLLATGVMVLHFILVSHLQPFSRYAYRAAAFAVTNASFQTLLRPQQFITLGRNTYR
                     VEALEAEGERFAGIFLYSEANEAGSVTVTAAAGEIESRGALVPLVLHLQNGVQQFVPR
                     PMDQTEASGNPPPPLTLRFGSFTSDLSGTEPEPFRPRGENERELTIPELFDLLWAPAE
                     TLPEEIDPWEIEAELSARLVRNLTILVLPFFALPMALSPRRAKRSYGLLVGIVLLVGY
                     NQLVNTGEALADDAKLSHWLSLWLPFLCFVTLSVGYFVRTARRVPDPGRSSLLGATID
                     GLVGTFLRRLPWRAA"
     gene            complement(11804..12787)
                     /locus_tag="P5558_04145"
     CDS             complement(11804..12787)
                     /locus_tag="P5558_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY23533.1"
                     /translation="MTVLDLAHFTDVHLGPLPQARLGELMGKRALGFLSWHHRRHRLH
                     TRGVLDCLVDDLQADPPDHVAITGDLVNIALPSEFELAARWLESLGPAEWVSLVPGNH
                     DAYAGRHLQRSWSRWHAWMRGDEPDSSGRFPFVRRLHDRLALIGLSSAVPSPVGQATG
                     TVGPAQLEALDGVLEGLGREGVCRILLLHHPPLGSMIRRRRALRDEAGLRKVIARRGA
                     ELVLSGHEHFFLFGGMPGKDAPVPVFVGPSASLAHGPLRTGGYLRHAIDLSGNAPRFT
                     VQLRHCEPGTGAIAVAQTARVECTGSGLELAPADWPKGRGSATESFPIRSH"
     gene            complement(12784..13542)
                     /locus_tag="P5558_04150"
     CDS             complement(12784..13542)
                     /locus_tag="P5558_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY23534.1"
                     /translation="MAAGMAALVLAGQRPEGDPMAAAAGIALKALLPVAGKPMLERVL
                     EALRAAPSIGRIAVSIPDPATLAGRDTEAVPTAASPSLSVLAALEHLPPPVLVTTADH
                     ALLTPAIIEAFLAGAAAIEADLVVGVVERRHIEAVVPETCRTYWRFRDGAYSGANLFH
                     LRNEQALAAIAFWRRAESERKRPWRIAGTFGPGLLLGYLLRRFTLAEALRRASAVVGC
                     RVAALVLPHGEAAIDIDKPADLVLVERLLKARPA"
     gene            complement(13544..14692)
                     /locus_tag="P5558_04155"
     CDS             complement(13544..14692)
                     /locus_tag="P5558_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP pyrophosphohydrolase"
                     /protein_id="HRY23535.1"
                     /translation="MSSRHATVAIQTVHDRADLKAFFDVTRHVYRDDPNWVQPLTLER
                     LDHLNPRKNPFLDGIEAAYWIVHLDGRPAGRISAQVNRAHLERHHDATGHFGFLEAID
                     DEEAFGLLIETAETWLGLRQMERMQGPFSLSINDETGTLVEGFTTPPSMMMPHGRPYY
                     DARLAEQGLAKAKDLIAYRYDNTQPLPPTARRLIERTAKTGGLVVRPLDMRRYVDEIR
                     LICDIFNDAWAGNWGFIPFGDAESQYLAKAIRPLVTAGCFAIAELDGEPAAMTVTLPN
                     LNEAIADLDGRLLPFGWSKLLWRLKVSGVTSARMPLMGLRRKWHGTLKGTAAALGVID
                     AVNRYHTSQGVREGELSWVLEDNTAIQRIIDAVGATAYKRYRVYEKAL"
     gene            complement(14720..15481)
                     /locus_tag="P5558_04160"
     CDS             complement(14720..15481)
                     /locus_tag="P5558_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRY23536.1"
                     /translation="MLTSWGPWSDKVYFLDKMSLGDLVRAFFTYYAIQAYIVIALVAW
                     ALAFAVMDSLWQPLVAAVVTAAIYPLVEYLVHRFLLHSTGLFKHKSTARVWKRIHYDH
                     HQNPHDLAVLFGALYTTLPTIALIALPVGWLVAGPAGALSAFATACIVFCAYEFVHCV
                     QHLPFTPRKAWLRDLKKRHLAHHFHNEQGNFGITSNLWDHVFGTFYAQPKDMPRSATV
                     HNLGYTGEARRQWPWVAELSLDDETYAIRRRRRAA"
     gene            complement(15627..17867)
                     /locus_tag="P5558_04165"
     CDS             complement(15627..17867)
                     /locus_tag="P5558_04165"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HRY23537.1"
                     /translation="MPELPTLTDRLDAWPFFRADPTPVVLGVRPGATPSDLPPVRIFL
                     GTEEAQYRAERIFFYSIEQVRDPGRVYEIHLMKNVTGFDRAKWRTGFTNYRYAIPAFA
                     GGTGKAIYNDVDQIYLADPALLFDLDMGDHGYLAISAKDTSVMLIDCERMLPLWNRET
                     AARLGKHALIDKPSATPGLWGQLDGHWNARDQEYVEGLTKCLHYTALHQQPWHPFPEA
                     FSYHPNPLAYVWYDLERAADAEDYEAFTNETPSPDFAAAIGRNLPSGEPAPAPGDEAV
                     ALFAALDVQDVLDVRLGPDADTPAPLTQGRRTTVLRLGQATPWPEKAHDAVVASDLLG
                     RVPPADLPWLLGRLFGAARKVVHLDAIANAPEGLGSAVWWRQRVDEIAARHPGVSWQL
                     TVADKAAPIPGTVRHFPVRAVAHPEQATIWALIDGVPAHDAQVRRLAAALGGAAIEQK
                     LDRPAIAFTAPWPDLVIAAGAKAASVARRIKGESQGATRIVQLGMPGAAFELFDLVIA
                     TPQLRLPVRDNVLQITAPLLPPTGEAAGSSGGTTALIGRGQKPFRLTAAAAARIGQAL
                     RDAGPGPKALRFEPDTPDEARDAVLAAAGEVEVIDPEKPLAEVMAKADRLLVTGDDPF
                     TLTAACLTGKPVTLVELPYWYDGLPGSKPIKTALTLLIGGGTSYRGTPHQQHVLGRLV
                     DRLIAKGWLSLPRDPARLHRALIARGLLVRADDPTPMAAPRPIDDLERVIERIRRMLT
                     RAPQPG"
     gene            complement(17913..19034)
                     /locus_tag="P5558_04170"
     CDS             complement(17913..19034)
                     /locus_tag="P5558_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY23538.1"
                     /translation="MATQPRRFFGVGARLNEVAGWVLLVLLGFGCVLVLRPFFSSLLW
                     AIILVFSTWPLHQRLKAMLGGHNLPASFLMTLALAAALIVPMALLVSALANTAEDAIS
                     QLRLSIHPDGTGAFAEPPAWLVGLPLVGNFLADLWRGLISNTGDLLASALPYFAPLRD
                     AAIRGGLGLGQGLIELAVSVLVAFFIYRDGAMAAELLHRSIGSVVGDRAKHLIAVAAG
                     TIRSVVYGVIGTALIQAMLALFGFWLLAVPQPFLLATCVFILGLFPVAGSSLVWIPVA
                     IWQLMTGQIVAGIIMGVYGALIIGGVDNFLKPYLISRGSQMPFLLVFFGVIGGVVTFG
                     ILGIFLGPMLLALGLSLTREWLQLQAGPPAVTSARIDGA"
     gene            complement(19040..19960)
                     /locus_tag="P5558_04175"
     CDS             complement(19040..19960)
                     /locus_tag="P5558_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRY23539.1"
                     /translation="MTISIGFVGLGSMGLPMAKQLLAKGHTVYGFDVAKKPLEELAAA
                     GGKPVASPRAAADQVSLLILVVATADQAETALFGKDGGAAVLGEGGVVVLHSTVPPPF
                     TKSLGERLAEMGLDLLDAPISGGQVGAVAGTLTVMASGSEAAFAAAAPALDAMAKTVY
                     RLGSEPGIGSTVKMINQHLAGVHLAAAGEAMALGAACGADPQTLFEVISNSAGNSWMF
                     SNRVPHILARDFTPHSAVEIFVKDLGIVLDTARGQRFPLPIAAAAHQQFLAAAGAGYG
                     REDDSAVVKVYERLRGFEVAGKTGGEGGKD"
     gene            complement(19957..20781)
                     /locus_tag="P5558_04180"
     CDS             complement(19957..20781)
                     /locus_tag="P5558_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015073571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRY23540.1"
                     /translation="MRVAVIQMNSGPDKAANLKAAKALIERAIERDRPDLVSLPETFT
                     IMGGGNAGRRAAAEAIPGGAAWTMLRDLAREHRVNVHGGSFFEDLGEGRLANTTVVID
                     RTGEEVARYRKIHLFDVETPSGEVYRESDYTVPGDRVVTVDLDGVRVGLTICYDLRFA
                     ELFLALRRAGAELILVPSAFTLPTGKDHWEVLLRARAIETQCYVAAAAQVGSFPAGSG
                     LRHSYGHALVADPWGHVVARVSDRPGHATAELDPAYLQLVRQRLPVERHRRLGQPG"
     gene            complement(20787..21788)
                     /locus_tag="P5558_04185"
     CDS             complement(20787..21788)
                     /locus_tag="P5558_04185"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRY23541.1"
                     /translation="MAVEKWGEFEGEPVGRVVLDNGKGLRAAILSWGGVLQSLTIDVD
                     GRPRPLVLGFDGFPDYPAKSPYFGATVGRFGNRIGHATFTLDGVRHDLEANMGGVHHL
                     HGGSKGWGRRLWRMTPGGDGRSVTLELTGPAGEAGYPGTVEARCLYQLTDDSLDIEMT
                     ATTTAPTPINMVHHTYWNLDGKGSIEAHELRLAADGYLPTGEGQIPTGEIRPLAGTGF
                     DFREARRIDAKGTADYDHCFVVNGSGLRPVADLVASDGRVRMTLDADQPGVQLYTGFK
                     MDITASDGAHIGPRAGLCLETECFPDAPNKPEFPSSILRPNETYRHRMVHRFAVAGA"
     gene            complement(21843..22586)
                     /locus_tag="P5558_04190"
     CDS             complement(21843..22586)
                     /locus_tag="P5558_04190"
                     /inference="COORDINATES: protein motif:HMM:NF023984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HRY23542.1"
                     /translation="MRWRPPLLLVLALVILPVGVSAWRQGAVAHWGGGRWDSTGMAPD
                     PVTATEAVVQVYCAASLGLKGAVADHCWIAMKPENATGYTRYDVVAWDLRRGGEAIRV
                     NHRGVPDGNWAGNRPRLLLDHRGAAAAALIPEIEQAVTDYPYRHTYRMWPGPNSNTFI
                     AWIAREVPGLGLDLPPRAIGKDYLGPRILAPAPSHTGFQLSLGGVFGLMVARAEGLEL
                     NFSGIVLGINPLDLEITLPGLGRLSLLGD"
     gene            22664..22996
                     /locus_tag="P5558_04195"
     CDS             22664..22996
                     /locus_tag="P5558_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY23543.1"
                     /translation="MTKRAPPGSHMVVMAGLVGALLPATLAAAEMVERGRIIAETFCA
                     QCHATGATGASPLPGAPPFRTFKERWPVEVLAEALAEGLTTGHPEMPTVTMTPGEIDA
                     FLGYLDSF"
     gene            23048..25429
                     /locus_tag="P5558_04200"
     CDS             23048..25429
                     /locus_tag="P5558_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY23544.1"
                     /translation="MSEKLGLIVEDSLSEIYVFNADTYEFVLVNRGARENLGYTMDEL
                     RELTPWDLKPEFTREAFVRMVEPLHAGAERFLTFETRHERKDGSVYDVAVKLQLIRGD
                     DDVFYAAIEDVTERKKVTEELRQTNRRLDAILNNTKMAVFMMDDRQHCVFMNKAAEEL
                     TGYTLEETLGRPLHDVIHHSYPDGRPFPLAECAIDSAFPQGNQMEGEEVFVHKDGSFY
                     PVAFTASPIRDDAGQPVGTVIEVRGIAEDLRTREAMRAFNETLQQRVAEAVAEQRKAE
                     LQLAQAQKMEAVGKLTGGVAHDFNNLLQVIGGSLQLLTRDVAGNERAEGLVRNALVGV
                     TRGANLASQLLSFGRRQPLEPRPINLGRVLRNLDDMLRRALGEEIEIETVVAGGIWNC
                     LADATQVENAILNLAINSRDAMGGRGKLTLEAGNASLDDAYAAEHAEVTAGQYVMLAV
                     TDTGSGIPADVLERVFDPFFTTKPEGEGSGLGLSGVYGFAKQSGGHVKIYSEEGQGTT
                     VRLYLPRTRRPEDSVPEGVAEEVTGGVETILVVEDDEAVRATAAEMLKELGYRVLKAK
                     DADSALAIVESGMAIDLLFTDVVMPGKLRSTELADHFRRRSPGGAVLYTSGYTQNAIV
                     HAGRLDDEVELLIKPYGREALARKVRQVLDASSATAQPPAGTEVEPRSRRSVLVVEDE
                     VLIRINTVDMLTELNFLVAEAATAREARTLLGRKSFDLLLTDVALPDGSGLELAREAV
                     ARHKELRVVLATGQVMEAMEDLPGASWLPKPYDERALAVAVGTWPARSDGGGS"
     gene            complement(25426..>25758)
                     /locus_tag="P5558_04205"
     CDS             complement(25426..>25758)
                     /locus_tag="P5558_04205"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRY23545.1"
                     /translation="VVDPTHPIVAGLPEQIVIEHEEMYGEHFDIPPPDELFLVSWFRG
                     GEVFRSGCTWRRGQGRIAYLRPGHETYPTYHNAQIQMLILNAVRWAAPVGAATPRRGN
                     QQPQEELG"
BASE COUNT         4007 a         8465 c         9464 g         3822 t
ORIGIN      
        1 gccaagtcca agccgaaata cagcatggcc ctgcattcct tcacacgctc tcagttccgc
       61 ccgtagaagt ggtgactgcc gagggtcgcc gtgcgctgca cctggtcggc catccaggac
      121 ggcttttcgc ggaccgtgcg gtggaaccag agggcacccc tggtcgggtc ggccggtcgg
      181 tcggggctgg ccatcagctc ggtgacgtcc cgggcgagcg cccacatgtc ggcctctttc
      241 ggctggtccg acttgccgtc gcagagccag ccccatttgc accggctgtc ggcgccttcc
      301 gtgatgacgc cctcgacgct gtccgggtat tcggggtcgg cggcgcggtt gagcacgacc
      361 cagcccacgg cgatcatgcc gtcgcgaccc tccgagcggg cctcgtgata gaggttgagg
      421 gcgaagagct cgctctcgtc gctgtcgatc accggctccg gcaagccctg atcggccgct
      481 gccgcgaccg ggccgatggc caagaacccc agggcgacca gtgggacgac ggcgagcgcg
      541 gcgaatgcgc gatgcatctg gttccttcat cgaccgggcc ggccaggcgg ccgaccaact
      601 ccgatgcgac cggagcagac ccgaccgcga gttcaggcta accaccatat ctatggtcaa
      661 tcgcgcgctc gaatgcaacc gatagtggtc aaggccattc cggcggtcat gggagccggc
      721 ccattcccgg ggcgggcggc ccgccggatg ggggcgggcg gcatgtcacc cgtcagccac
      781 aggatcggcg aaaacgtcgc aggttctttg ctgcagtgca caataaacca actatacgag
      841 tgacgcgagc gggcggttcg cgctaaccca tgcggcattc ccggggaggt tgagcggagg
      901 tatgagcaac cccctgtaac aatctgcaac gcaacgtgat tcgtgggtga acaaccgttg
      961 ctctaccgtc caccatcgaa acagaccggt catcgatgcc agggcagtgg cggaaccggt
     1021 cgaaagctgg gcccgttttg atcggccatg gggatcgtca ggtcgccacg gtcgggcggg
     1081 tggacccggc gaaacacgct gccgggtgtc ggcgcagtca ttgctgcagt cggcacagga
     1141 ccagcctagt cggagttgag tgatgaacga cgtaacgccc accgcgaatg agcgactgcc
     1201 cttccgggcg ggcgatttcg tcacgctcgc cgaggggctg gattacgcgg ctcgcggcga
     1261 gaccggcgtc aacttctttt cgacccgcgg ctcgctcgag acggcgctgc cctattccga
     1321 gctgcgggag cgggcgatcg ccctcgcccg gcggctggtc ggcacgggcc tccggcgcgg
     1381 cgaccggatc ggcatcatcg ccgagacctc gcccgacttc ctcgccgtct tcttcgcctg
     1441 ccagtatgcc gggttcgtgc cggtgccgct gccgctcagc atcaatttcg gcggccgcga
     1501 ggcctatgtc gagcggctgc gcggcatgct cgccgctgcc ggggcgcgcg ccgccgtggc
     1561 cggtcccgag ctcgtccagg tgctggccgc cgccaccgag gcgaccatcg tccgcttcac
     1621 cgcgaccgcc gacgagctca tggcgctgcc ggtctcggac cagcccctgg aggcgctcgg
     1681 cgcagatgac gcctgctaca tccagtacag ctcgggcagc accagcttcc cgcgcggcgt
     1741 gctggtcacg cagcgggcgc tggccgccaa tgcccggtcg atcgccacgg acggcctggc
     1801 gctcgggccg accgaccgct gcacctcctg gctgccgctc tatcacgaca tgggcctcgt
     1861 cggctgctgc ctgacgccgg tcatgtgcca gatctccgtc gactatctgg ccaccactgc
     1921 ctttgcccgg cggcccctgg tctggctgca gctgctggcc aaacatggcg ggacgatctc
     1981 gttcgcgccg accttcggct acgagctctg cgcccggcgg gccgccaatg gcgccgcgcg
     2041 cgacctcgac ctcagccgct ggcgggttgc cggcatcggt ggcgagatga tccgctcgcg
     2101 ggcgctcacc gccttcgccg atactttcgg cgcttgtggc ttcgacgccc gggccttcgt
     2161 gccgagctac ggcatggccg aggcgacgct ggccgtcacc ttcaacccgc tcggtcgtgg
     2221 cgtgcggatc gatcgggtgg cgcgcggcga gacgctcgag cgccagcgcc tggcgctgcc
     2281 ggctggcgag gccgtgccgc cgtcggagag ccgcagcttc gtcgtctgcg gcgccccgat
     2341 gccgggctat gccatcgaga tccgcgacga ctcgggccgg gtgctgcccg agcggcatgt
     2401 cgggcgcatc tgcatccagg gcccgagcct gatggagggc tatttccgca atcccgaggc
     2461 gaccgccgcc gtgctcaggg gcgatggctg gctcgacacc ggcgacatgg gctatctcgt
     2521 cggcggcgag ctcgtcgtct ccggtcggac caaggacctg atcatcgtcg gcggccgcaa
     2581 catctggccg caggacatcg aatgggcggt cgagaagctc gacgccgttc gggccggcga
     2641 tgtcgccgcc ttcgcggtga ccgacagcct cgatcgcgag cgggtcgtgg tggtggtcga
     2701 gtgccggcag acgcggccgg ccgagcaggc cgagctgcgc cacgaggtcg ccgccgtggt
     2761 caacaggctc gccgggatcg agtgcgaggt cgtactcgcg gcaccgcggt cgctgacctt
     2821 caccacctcg ggcaagctga gccgggccgc cgcgcgcgac cactaccagc gcggcgtgct
     2881 gcgcgacctc gacgccgcgg ccgccgatga cgccttcgac ggtgccgtcg agcccggcca
     2941 gccggcggtg acggcgctcg ccgaggcgag ctgacgacgg ctgcaggccc cggcgccgca
     3001 gccccggccg gtcgggcccc cgggacggcc gcaccacctc gattggcgac agcagaggga
     3061 gcgatgacgc gcaccgtggc gctcaccggt gccaccggct tcgtcggccg gcatctcgtg
     3121 gcccggctgg gccaggccgg tgtcggcgtg cgcgccctgt cgcgccgtgg gccgatcgag
     3181 ggtgcggtga ccgtcgccgg cgacctcgcc gaccgggcgg ccctggacga gctggtccgg
     3241 ggtgcctggc gggtcctcca tctggcgggc gtgatcaaga ctccgcgacc ggccgagttc
     3301 accgccgtca atgtcgaggg tacccggcac gtcgcggagg ccacggcgca ggccgggctg
     3361 aagctcaccc tcgtctcgtc gctggccgcc cgccatccgg aggtctcggc ctatgcggcg
     3421 agcaagcgcg cggccgagga gacggcccgg gccgtcatgc cggagggcga tctcacgatt
     3481 gtccgcccgc cagccgtctg ggggccgggc gaccgggcga cgctggtcgt ctttcgccag
     3541 ctccttgccg gctggctggt cgtgccggca ccgaaaggcg cgcggttctc gctgctgcat
     3601 gtcgcggatc tggtcgggct gctggtcgac cacgtgctgg atcggctggc gctgccgccc
     3661 ctcgccgagc ccgatgacgg cacgcccggc ggctatgggt gggacgagct cgcccggacc
     3721 gccgagcggc tgaccggccg ccgggtccgc ctgctgcggc cgcccacggg cctcctcgcc
     3781 gggccggcgc ggctcgccga tctcatcgcc cgggcgaccg gcgccgacct gccgctctcg
     3841 gccgacaagc tcggcgagct ggcgcatgcc gactgggtcg ccgccggccc ggcattgccc
     3901 tggagggcat cgcgcatcac attcgaccag ggtttcgccg cgacggtcga ctggtaccgt
     3961 gcggccggct ggctgccggc cacggcgacc cgaacggcag atggaagcaa ggcatgaccg
     4021 agaacgtcct gaacgagatc atggcgctga tgcagcgcta caacccggac ggcaagccgc
     4081 tcgacgccgg gaccgagctc ggcgccgatc tgaacatcga ttcggtggcg gccatggacc
     4141 tgatcatgga aatcgaggac aagttcgaca tcgacatccc gatgaaccag gtggccgagc
     4201 tcaggaccat cggcgatctc gtggcgctgg ccgagggtca gctgcccaaa ggctgagtcg
     4261 acaggctcca aaccagctcc aagggtgccc gagcacatgg acatcttcga caagtatgcc
     4321 ggcatcgagc agcgccaccg ggccctgctc tcgatcggcg gcgatccctt cgccgtccgg
     4381 gtcgaccgca tcatctcggc gaccgaggcc atgatcggcg gccgccgcac catcctcgtc
     4441 ggcaccaaca actatctggg cctcaccttc gatccggcct gcatcgaagc cggccgcgag
     4501 gtgctggcga ccgatgggac cggcaccacg ggctccaggg tcgcgaacgg cacctatggc
     4561 cgccacaccg atctcgaggc cgccttcgcc cgtttcttcg atcgccagaa ggccatcgtc
     4621 ttcaccacgg gctaccaggc caatctcgcc accatctgcg gcctcgccgg gcccgacgac
     4681 gtgatcctgg ccgacgccga cagccacgcc agcatctggg acggctgcaa gctctcgccc
     4741 gccgaaaccg tggtcttccg ccacaacgac ccggaccatc tcgacaagcg gctgacccgg
     4801 ctcgccgaca agggccagtg caagctggtg gtcgtcgagg gcctctactc gatgctcggc
     4861 gacaccgcgc cgatcaagga gttcgtcgag gtcaagaaga aacacggcgc cttcctcctg
     4921 gtcgacgagg ctcattcctt cggcgtcttc ggcgacaagg ggcgcggcgt cgccgaggcg
     4981 cagggggtgg aggcggatgt cgacttcgtc gtcggcacgt tctccaagag cctcggcggc
     5041 agcggcggct tcgccgtctc cgaccacccg aagttcgatc tcctgcggct cgccagccgg
     5101 ccctacatgt tcaccgcctc gccgagcccg gccaccatgg ccacggtttc ggaggcgctg
     5161 cgccagatcg aggccaggcc ggagctgcgc acgcgcattt gggccaatgc cgagcagctg
     5221 catacggggc tgagggacct gggcttcacg ctcctcgccc cgctcagccc ggtgatcgcc
     5281 atccgcatgc cggacgaggc cacggcggtc atggcctgga accgcctcat cgaggccggc
     5341 gtctacgtca acctggcgct gccaggcggc acgcccggcg gcgtctgcct gctccgctgc
     5401 agcgtctccg ccgcccacga gcccgcccag atcgccacgg tgctcggctg cttcgcggag
     5461 ctggcgagcg agctggaccg gctgaagggg cagaacgcgg cgtaggggga gcggtgtccg
     5521 tcgggcaccg catctcgagg gcgggcgggt gacagggggc tcgtgcaccg gttttcgcgg
     5581 ccggcttgct gcgctcgcca cgcgggtgtt atccagagcg acgaacgttc gacatgcccg
     5641 cacacgcgcc gccacccgct attgtcacct ttcaatgccc atgtcgggtg aggtgcgcgt
     5701 gacgaacacc aagaagcgcg ttggagaggc cacatgacac gcgtggccat tcgccctgaa
     5761 cttttgcgat gggcgcggga gcgagcgggc attcatgacg tcggtgacct tatcagtcgt
     5821 ttcccgaaac ttggcgattg ggaaaccggt gaagcgcaac ccacgctcaa gcaactggag
     5881 gcgttcgcgc agacgcttca tgtgcccatt ggttatctgt tcctgtcgga accacccgta
     5941 gagcggttgc cgatcccgga tttccgtacc catggcgggc gcggagtgcg gagcaccagc
     6001 ccggatttgc tcgacatgct ttacgcctgc caggaacggc agggctggta tcgtgacttt
     6061 gctctgacgg tccgtatgtc cgaggcagcc ttcgtcggca gcgcagacct cggtgaaagg
     6121 ccacaagacg tggcggcgcg tatggctgag acgctcggct tcgatgtggc cgcgagggcc
     6181 gcctgccgta cctgggaaga ggcattgcgg cttttcattg cccaggcgga tgctgccggc
     6241 gtgctggtga tggtgagcgg tgttgtgctt tccaacaacc gccgcatcct tgacccagag
     6301 gagttccgtg gcttcgcttt ggccgacaag cgcgcgccac tggtattcat taacggagcg
     6361 gacacgaaat ctggccagat gttcacgctg gcgcacgagc tggcgcatct ttggctaggg
     6421 tcctccgcga tctcggatgc cagcgccgca ccgctctacg gctaccgccg cgaggaggtc
     6481 tggtgcaatg cggtcgctgc cgagcttctc gtgccccttg cggtgcttcg ccccgcgatc
     6541 gagcgggacg agcccctcga tcgagcgatg cagcggctgg cgcggcagtt caaggtcagc
     6601 acgcttgtca tcctgcgtcg gctgctggac gcgggcgcgc tcgaccggcc ggccttcgac
     6661 cgcgcctggg cagaagaacg cacacgcctg cgtgaacttg cccgatccgc cggcggcggt
     6721 ggcgacttct accggacgac gctttcgcgc gtcagccgcc gctttgcgcg tgcgctcgtc
     6781 gagagtacgc tggaaggaca gacgctgtac cgcgacgcct tccggatgct gggcgtcaag
     6841 aagacggaga cgttcaacaa tctcggccgc gaagtggggg tcttgggatg acccgccacc
     6901 tgctcgacac gaacatcttc attcaggcga agaacctcca ttacggcttc gatttctgcc
     6961 cggcgttctg ggaatggctg atcgaacgga acgtcgccgg ttgcgtcgct agcatcgaca
     7021 aggttggcga tgaactgcag gcgggcggcg acgatctttc cgactgggcc actgcacgcg
     7081 ggcgcgcttt cttcttgccg cctgatgacc ccgtcctgcc tgcccttggc cgagtgagcg
     7141 cctgggcgac cagccggaac tacgaggcgg cggcgatcgc aacttttttt caggtggcgg
     7201 attactggct ggttgctcac gctctggcac acgactttgt tgtcgtaacc cacgaggtac
     7261 cgtcggactc gacccgcaag atcaagatcc ccaacgcctg catcgggttg ggccttacgt
     7321 gcatcagccc ctatgagatg ctccgtcgcg aacgcgcccg gttcgttctg gggccagcgc
     7381 cgcgggtagc atgagctcag attgatcgac cggtcttaga accttctgca cgctggaagc
     7441 gcgcgtttat actccagcag gcttcgcaac gttgaagtgg aaaaacaaac aaggaggtaa
     7501 ggtgccctgc gtggccctgc tccgctgcag cgtctccgcc gcccacgagc ccgcccagat
     7561 cgccacggtg ctcggctgct tcgccgagct ggcgagcgag ctggaccggc tgagcaagca
     7621 ggacgcggcg tagtcatcga accctaccgc accgccttgc tgcgccagag gcgccagagg
     7681 atgggggggg cggcgaggat ggggtggcgg cgttgccagt cggtcagcgg gacgggctgg
     7741 ccggagtggc gctcgatctt ccaggcgagg tagtccgcac cgccgctgaa ggtgaaggcg
     7801 gccttcatga ggcggaggat ggagagcgcc ttgccgaccc gcctcgtgcg cgcccaggag
     7861 cggtggcggg gcctgacgtt ctcgccgatc agcgcgcggg tgagccggtc gtagcgggcg
     7921 gcgaagctct ggtagatctc ggcggcgcgg tcgggcttct cggcccgaag ctcggtgcgg
     7981 taggtctcga ggaagaccac ggtccagagc gcttccgagg ccgcgcggtc gcgggtcagg
     8041 gcgcgggcgg cggcgaaggt ggtgcgcacg gcctcggcca ggatgttcgt gacccgccgc
     8101 cgcgtcgcgt cgttgcggca ccagacgagg ccggtcggct gggcgaagcg ggcccagaaa
     8161 taggggttgt cggtctcggg cgagactttc gcctcgagct ggtcgagggt gacgatggcg
     8221 tacttggcgc gcacggtttc gccggcgtgc tcgatctcga gatagaagac gttgggcggg
     8281 accagcctgt tggcgcgggc ggcgaggcgg ccctggcctg tcctggcgta gtcgtcggcg
     8341 agcagataga ggtcgacgag gcggtccatg accacgccat cccgaaagca gctgccgtag
     8401 aagagcacgg cggcgatgcc cggcgcatgg cgctcgcagg ccgccctggc gatggcctgc
     8461 accgcctccg gtgccggggt cgcgagctcc gcccggagca ccgcctcgag atcgcctctt
     8521 gcggccgcca tcgtcagacc cgcgcgaagc ggatggtttc gtcggcggtc acggtgaccg
     8581 gccggccggg cgccggcgtg aagaactcgc cgtcgatggt gaaggggccg tcgagccaga
     8641 gctcgagccg ccggccgccg ccgctgcggt aggagtcgct cggcaaccgc cggtcggcgc
     8701 cgccatagag cagccgccag gcgtgccgca cgaggcccct gggcggatgg gcgaaccggg
     8761 tgtggcggag cggcaggtcg ccagcacccc aatagggtct ggagcggagc accaggcggt
     8821 cgagcgtggt ggcgatcagg atggcgagca cctcgtcccg gcgggccgtg ccatcgatgg
     8881 cgagcccgat ccggtgggcg cgcaggccca gcgcctgaaa gccgcgaaag aggcagaggc
     8941 cgagcaggcc gaggatcgtc gccgcgtgcg accactcgcc cttgatgccg cggctgtgca
     9001 ggcggccggt gacgaacagg atgatgtcga tgatgccggc ggcacccatg aacatgccgc
     9061 gcggagcggg gaccccgtcg gcgaaatcgg ccttgaggat gtggcgggtg acgagcgaat
     9121 cggcgagccg gcccgcccgg caggcggcga ccagccgttc cagcgtggcc gcgtcgtggc
     9181 cggcgaggcc gcaatcggtg gcggtcatgt tggccatgcc gcgcggcagg atggcgagcg
     9241 ccggcagctc ggcgaacggg tggtgcgcca agagctcggt caagataccc tgcaccgtgc
     9301 cgtcgccgcc gctgacgacg acgaggtcgc agccggcagc ggcgaagctc tgcagcggcg
     9361 ggccgaagcc cagggcgggc tcgaaccggc ggtgcaggat ctccggcgtg cgctcggcca
     9421 gggcctcgat cgccgccatg cccacgcggt tgcgggtgct gcccggattg ctcaggaggc
     9481 cgatgcgcat caggcggccc ggcggcgcag cagcagctcg tccctgagcg gcaggccgac
     9541 gacgagggcg agcagcagga gcatcggccc ggcggtggcg gcgagcggcg ggaggaatcc
     9601 ggcctcggcc agcgccagcg ccaggccgtt caggatgacg aagacgaagc cgccgccgat
     9661 gcccttcatg aagagggtgg aaatggcgcc ctggcgggtg gagcggcgcg ccagcgcaaa
     9721 ggtcagcatg accaggagcg tgggcagggt ggcgccgaag aggcggtgga agagggcgct
     9781 ctcgtggccc tgtgtcgcca ccaccccgta gccgtcgttc ctgatgatgt cgacgaggtc
     9841 gaggaggccg atctcctgtg gcgggcgggc gaccaccctc aggcgatcga ggtcgatctc
     9901 gttctcccag acgagctccg gctcggtggt gaagccggcg ccgaccgtcg cccgctgcac
     9961 gtcgagcagc cgccaggcgc cgggcaggag ctcggccctg gctgcggtga gccgctcgac
    10021 caggttgccc accgagtcgc gctcgaggat gatgacgttg ctggcgctgg tggcgcccgg
    10081 ctcgagccgc ggcaggcgga cgaagtcgcc ccggtgctgc atccagatgt cgttgccgac
    10141 gaagccgtcg agcgtgctct tgaacgagcc gacctcccag gcccgaagct cctggatcgt
    10201 cggcggcacg aggagctcgt cattggcgag ctcgagcgcg cagatggcga gcccgatcgg
    10261 caggaggcgc cggacgatgc cgagcggcga gacgcccgac gcccagagga cgatgaactc
    10321 gctgttgcgc aggagctcgg cggcggcgaa gagggcgccg agcaatgccg cgaacggcag
    10381 gacctcggcg accaggctcg gcaggcgcag ggcgaaatag cggccgaagg cgagggcgac
    10441 gttgccttcg gtgcggatga tgtcgtccga ggcgtcgagg aggtcgaaga ccagcgcgaa
    10501 gaggctgatg gcgatcaccg ccgccgtcac gcggagcagc accatccggt tcaggtaggc
    10561 ggtgagcacg ctcatgcggc gcgccatggc aggcggcgga gaaaggtgcc gaccaggccg
    10621 tcgatcgtgg caccgaggag cgagctgcgg ccgggatcgg ggacgcgccg cgcggtgcgg
    10681 acgaagtagc cgaccgagag cgtcacgaag cagaggaacg gcagccacag ggagagccag
    10741 tgggagagct tggcgtcgtc ggcgagcgcc tcgccggtgt tgacgagctg gttgtagccg
    10801 accagcagca cgatgccgac gagcaggccg taggagcgct tggcgcggcg cggcgacagc
    10861 gccatgggga gggcgaagaa cggcaggacg aggatcgtga ggttgcgcac caggcgggcc
    10921 gatagctcgg cctcgatctc ccaggggtcg atctcctcgg gcagcgtttc ggccggggcc
    10981 cagagcaggt cgaagagctc ggggatcgtc agctcgcgct cgttctcgcc gcgcggccgg
    11041 aagggctccg gctcggtgcc cgagagatcg ctggtgaagc tgccgaagcg aagcgtgaga
    11101 ggtggtggtg gattgccgct ggcttcggtc tggtccattg gtcgcggcac gaactgctgc
    11161 acaccgttct gcagatgcag gaccagcggc acgagggcgc cccggctctc gatctcgcct
    11221 gcggcggcgg tgacggtgac cgagcccgcc tcgttcgcct ccgaatagag gaagatgccc
    11281 gcgaaccgct cgccttccgc ctccagcgcc tcgacccggt aggtgttccg gccgagggtg
    11341 atgaactgct gcggcctgag cagcgtctgg aacgacgcgt tggtcacggc aaaggcggcg
    11401 gcccggtagg cgtagcggct gaagggctgg aggtggctga ccaggatgaa atgcaggacc
    11461 atcacgccgg tggcgaggag gacgagcggc cgagcgatcc gggcgaggcc gatgccggcc
    11521 gcgcgcatgg cgtcgagctc gccttcgcgc gacagccggc caatggccag catgatgccg
    11581 aggaagaagg cggcaggcag ggcgaccacc atgtagtgcg gcacgaggta gctcagcatc
    11641 tcgaagacga tggcgagcga gccgcgccag ccgatgacga ggtcgacgac gcgcagcgcc
    11701 cgctcggcga gcagggcgag gagcgtgatc aggatggcgg ccagcatcgg ccgcagcacc
    11761 tgaccgagca catagcggtc gagcgtgccg agcatcggtt gggtcagtgg ctgcgtatcg
    11821 ggaagctttc agtagccgag ccacggcctt tcggccagtc ggccggtgcc agctcgaggc
    11881 cactgccggt gcattcgacc cgggcagtct gcgccacggc gatggcgccg gtcccgggct
    11941 cgcagtggcg caattggacg gtgaagcgcg gcgcgttgcc cgagagatcg atggcgtggc
    12001 ggagatagcc gccggtgcgc agcggtccgt gcgccagcga ggccgagggg ccgacgaaca
    12061 ccggcaccgg ggcgtccttg ccgggcatgc cgccgaagag gaagaaatgc tcgtggccgg
    12121 agagcaccag ctcggctccc cgccgggcga tgactttcct gagccccgct tcgtcgcgca
    12181 gcgcccggcg gcggcggatc atcgagccga gcggcgggtg gtgcagcagg aggatgcggc
    12241 agacgccttc ccggccgagc ccttcgagga caccgtccag cgcctcgagc tgggcgggtc
    12301 ccaccgtgcc ggtggcctgg ccgaccgggc tcggcacggc gctcgacagg ccgatgagcg
    12361 ccagccggtc atggagccgg cgcacgaacg ggaaacggcc ggagctgtcc ggctcgtcgc
    12421 cgcgcatcca ggcgtgccag cgcgaccagc tccgctggag gtgccggccg gcataggcgt
    12481 cgtggtttcc cgggaccagg ctcacccatt cggccgggcc gagcgactcg agccagcgcg
    12541 ccgccagctc gaactcgctc ggcagggcga tgttgacgag gtcgccggtg atcgcgacat
    12601 ggtcgggcgg gtcggcctgg aggtcgtcga ccaggcagtc cagcacgccg cgcgtgtgca
    12661 gccggtggcg gcggtgatgc caggagagga agccgagcgc ccgcttgccc atgagctcgc
    12721 cgagccgcgc ctgcggcagc gggccgagat ggacatcggt gaaatgggcg agatcgagga
    12781 cggtcatgcc gggcgggctt tcagcagccg ttcgacgagg acgaggtcgg ccggcttgtc
    12841 gatgtcgatc gcggcctcgc cgtgggggag caccagggcc gcgacccgac agccgacgac
    12901 ggccgacgcc cggcgcagcg cttcggccaa agtgaagcgg cgcaagagat agcccagcag
    12961 caggccgggg ccgaaggtgc cggcgatccg ccacggccgc ttgcgctcgc tttcggcccg
    13021 ccgccagaag gcgatggcgg cgagggcctg ctcgtttcgc aggtggaaga ggttggcgcc
    13081 ggaataggcg ccatcgcgaa agcgccagta ggtccggcat gtttccggca ccacggcctc
    13141 gatgtggcgg cgctcgacca cgccgacgac gaggtcggcc tcgatggcgg cggcaccggc
    13201 gaggaaggcc tcgatgatcg ctggcgtcag cagggcatgg tcggcggtgg tgaccaggac
    13261 cggcggcggc aggtgctcga gggcggcgag cacagagagg ctgggcgagg cggcggtcgg
    13321 gacggcctcc gtgtcccggc cggccagggt cgcgggatcc gggatcgaga cggcgatccg
    13381 gccgatcgac ggggcagccc gaagggcttc cagaacccgc tccagcatcg gctttccggc
    13441 caccggcagg agggccttga gcgcgatgcc ggcggcggcc gccatcggat cgccctcggg
    13501 gcgctggcca gccagcacca gggccgccat gccggccgcc atctcagagc gccttctcgt
    13561 agacgcgata gcgcttgtag gctgtggccc ccacggcatc gatgatgcgc tggatggcgg
    13621 tattgtcctc gagcacccag gagagctcgc cctcgcgcac gccctggctc gtatgatagc
    13681 ggttgacggc gtcgatcacg ccgagcgccg cggcggtgcc cttgagcgtg ccgtgccact
    13741 tccggcgcag gcccatgagc ggcatgcgcg cggatgtcac gccgctcacc ttcagccgcc
    13801 agagcagctt cgaccagccg aacggcagca gccggccgtc gagatcggcg atcgcctcgt
    13861 tgaggttggg cagggtcacg gtcatggcgg ccggctcgcc gtcgagctcg gcgatggcga
    13921 agcagccggc ggtgaccagc gggcggatgg ctttggcgag atattgcgat tcggcgtcgc
    13981 cgaaggggat gaagccccaa ttcccggccc aggcatcgtt gaagatgtcg cagatcaggc
    14041 ggatctcgtc gacatagcgg cgcatgtcga gcgggcggac gacgagcccg ccggttttcg
    14101 cggtgcgctc gatcagccgc cgcgcggtcg gcggcagcgg ctgcgtgttg tcgtagcggt
    14161 aggcgatcag gtccttggcc ttggcgaggc cctgctcggc cagccgggcg tcgtagtagg
    14221 gccggccgtg cggcatcatc atcgagggcg gcgtcgtgaa cccttcgacc agcgtgccgg
    14281 tctcgtcgtt gatcgagagg ctgaacggcc cctgcatccg ctccatctgc cggagcccga
    14341 gccaggtctc ggcggtctct atgagcaggc cgaacgcttc ctcgtcgtcg atcgcctcga
    14401 gaaagccgaa atgcccggtg gcgtcatggt gccgctcgag atgggcgcgg ttgacctggg
    14461 ccgagatgcg gcctgccggc ctgccgtcga gatggacgat ccagtaggcg gcctcgatgc
    14521 cgtcgaggaa cgggttcttc ctcgggttga ggtggtcgag ccgctccagg gtcagcggct
    14581 gcacccagtt ggggtcgtcg cggtagacgt ggcgggtgac gtcgaagaag gccttcagat
    14641 cggcgcgatc gtgaaccgtc tgaatggcga ccgtggcatg gcgggaggac aagcgctgca
    14701 gcccgtcgcg gccaccgcct caggcggcgc ggcggcggcg gcggatggcg taggtctcgt
    14761 cgtccagcga cagctcggcg acccagggcc actgccgacg cgcctcgccg gtgtagccca
    14821 ggttgtgcac cgtagcactg cgcggcatgt ccttgggttg ggcgtagaac gtgccgaaga
    14881 cgtggtccca gagattgctg gtgatgccga aattgccctg ctcgttgtgg aaatggtggg
    14941 cgagatggcg cttcttcagg tcgcgcagcc aggccttgcg cggcgtgaac ggcagatgct
    15001 ggacgcagtg cacgaactcg taggcgcaga agacgatgca ggccgtggcg aaggcggaaa
    15061 gagcgccggc cgggccggcc accagccagc cgacgggcag ggcgatgagc gcgatggtcg
    15121 gcagggtcgt gtagagggcg ccgaacagca ccgccagatc atgcggattc tggtggtggt
    15181 cgtagtggat ccgcttccag accctggccg tcgacttgtg cttgaagagc ccggtcgaat
    15241 gcaggaggaa gcggtggacc aggtactcga cgagcggata gatggcggca gtcacgacgg
    15301 cggcgaccaa aggctgccac aggctgtcca tgacggcgaa ggcgagcgcc caagcgacga
    15361 gcgcgatcac gatataggcc tggatcgcgt agtaggtgaa gaaggcgcgg accaggtcgc
    15421 ccaggctcat cttgtcgaga aaatagacct tgtcgctcca gggaccccag gacgtcagca
    15481 tgccagtcta ccgtaatttg gatgtgattt ccggctcatt cccgagttaa tgccagctcg
    15541 cccgaggggc aagggtggtg ccgcgctttg ccacggcagg gtggcgccga gatgacaccg
    15601 gccggtcgga ttttcattcc gccagttcaa cccggctgcg gtgccctggt cagcatgcga
    15661 cggatgcgct cgatcacgcg ctcgaggtcg tcgatcggcc tgggtgcggc catcggcgtc
    15721 gggtcgtcgg cgcggaccag gaggccgcgg gcgatcaggg cgcggtgcag gcgggcggga
    15781 tcgcgcggca ggctgagcca gcccttggcg atcagccggt cgaccagccg gcccagcacg
    15841 tgctgctggt gcggcgtgcc gcgataggag gtgccgccgc cgatcagcag ggtgagcgcc
    15901 gtcttgatcg gcttgctgcc cggcaggccg tcataccagt agggcagctc gaccagggtc
    15961 acgggcttcc cggtgaggca ggcggcggtg agcgtgaagg gatcgtcgcc ggtgaccagg
    16021 aggcggtcgg ccttggccat gacctcggcg agtggcttct cggggtcgat cacctcgacc
    16081 tctcccgcgg cggcgagcac ggcgtcgcgg gcttcgtccg gcgtgtccgg ctcgaagcgc
    16141 agcgccttcg ggccggggcc ggcgtcgcgg agtgcctggc cgatgcgggc cgccgctgcc
    16201 gcggtcagcc ggaagggctt ctggccccgg ccgatgagcg ccgtggtgcc gccgctgctg
    16261 ccggccgcct cgcccgttgg cggcagcagg ggtgcggtga tctgcagcac gttgtcgcgg
    16321 accggcaggc gaagctgcgg cgtggcgatg acgaggtcga agagctcgaa ggcggcgccg
    16381 ggcatgccga gctggacgat gcgcgtcgca ccctggctct cccccttgat ccgccgggcg
    16441 acggaagcgg ccttcgcccc ggcggcgatc accagatcgg gccaaggggc ggtgaaggcg
    16501 atggcgggcc ggtcgagctt ctgctcgatc gccgcaccgc cgagggccgc agccagccgg
    16561 cgcacctgcg catcatgcgc cggcacaccg tcgatgagcg cccagatggt cgcctgctcg
    16621 ggatgggcca ccgcccggac cgggaaatgc cgcacagtgc cggggatggg tgccgccttg
    16681 tcggcgacgg tgagctgcca gctcacgccc ggatggcggg cggcgatctc gtcgacgcgc
    16741 tggcgccacc agacggcgct gccgagcccc tcgggtgcat tggcgatggc gtcgagatgc
    16801 acgaccttgc gcgccgcacc gaagagccgg ccgaggagcc agggcaggtc ggccggcggc
    16861 acgcggccca gcaggtcgct cgcgaccacg gcgtcgtgcg ctttttccgg ccacggcgtc
    16921 gcctgcccga ggcgcagcac ggtcgtccgc cggccctggg tcaggggtgc cggcgtatcg
    16981 gcgtcgggcc cgagccggac gtccagcacg tcctggacgt cgagcgcggc gaagagggca
    17041 acggcctcgt cacccggcgc cggggcgggc tcgcccgagg gcaggttgcg gccgatcgcg
    17101 gcggcgaaat ccgggctcgg cgtttcgttt gtgaaggcct cgtagtcttc ggcgtcggcc
    17161 gcccgctcga ggtcgtacca gacataggcc aacggattgg ggtggtagga gaaggcttcg
    17221 ggaaaggggt gccagggctg ctggtgcagg gccgtgtagt gcaggcactt cgtcaggccc
    17281 tcgacatatt cctggtcgcg ggcgttccag tggccgtcga gctggcccca gagcccgggc
    17341 gtcgcggagg gcttgtcgat cagcgcgtgc ttgccgagcc gggctgccgt ctcgcggttc
    17401 cagagcggca gcattcgctc gcagtcgatc agcatgaccg acgtgtcctt ggccgagatg
    17461 gcgagatagc cgtggtcgcc catgtcgagg tcgaagagca gggcggggtc ggcgaggtag
    17521 atctggtcga cgtcgttgta gatcgccttg ccggtgccgc cggcgaaggc ggggatggcg
    17581 tagcggtagt tggtgaagcc cgtccgccat ttcgcgcgat cgaagccggt gacgttcttc
    17641 atcaggtgga tctcgtagac ccggccgggg tcgcggacct gctcgatcga gtagaagaag
    17701 atccgctcgg cccggtattg cgcctcctcg gtgccgagga agatgcgcac cggcggcagg
    17761 tcgctgggcg tggcccccgg ccggacgccg aggacgaccg gcgtcgggtc ggcgcggaag
    17821 aacggccagg cgtcgaggcg gtcggtgagg gtgggaagct cgggcatgcg ggcctttttc
    17881 ggttgcggct cggcgatcag gtaggctgcg ggtcaggccc cgtcaattcg agccgatgtc
    17941 acggcaggtg gccccgcctg aagctgcagc cattcgcgcg tcagcgacag cccgagcgcc
    18001 agcagcatgg gcccgaggaa gatgccgagg atgccgaagg tcacgacgcc gccgatgacg
    18061 ccgaagaaga cgagcaggaa cggcatctgg ctgccacggc tgatcaggta gggcttcagg
    18121 aagttgtcga ccccgccgat gatcagcgca ccgtagacgc ccatgatgat gccggcgacg
    18181 atctgcccgg tcatcagctg ccagatggcg accgggatcc agaccagcga cgagccggcg
    18241 accgggaaga ggccgaggat gaagacgcag gtcgcgagca ggaagggctg cggcacggct
    18301 agcagccaga agccgaagag cgccagcatc gcctggatca atgcggtgcc gatcacgccg
    18361 tagacgacgc tgcggatagt gccggcggcg accgcgatca ggtgcttcgc ccggtcgccg
    18421 acgacgctgc cgatcgagcg gtgcagcagc tcggccgcca tggccccgtc gcggtagatg
    18481 aagaaggcga cgagcacgct gaccgccagc tcgatcaggc cctggccgag gccgagcccg
    18541 cccctgatcg cggcgtcgcg caagggggcg aaatagggca gggccgaggc caggaggtcg
    18601 cccgtgttgg agatcaggcc gcgccagagg tcggcgagga aattgccgac cagcggcagg
    18661 ccgacgagcc aggcgggcgg ctcggcgaag gcacctgtgc cgtccgggtg aatgctcagg
    18721 cgtagctggc tgatggcgtc ctcggcggtg ttggcgaggg cgctcaccag gagcgccatc
    18781 ggcacgatca gcgctgccgc cagcgccagc gtcatgagga acgaggccgg caggttgtgg
    18841 ccgccgagca tcgccttcag ccgctggtgc aggggccagg tcgagaagac caggatgatc
    18901 gcccagagca gcgacgagaa aaagggtctc agcaccagga cgcagccgaa gcccaggagg
    18961 accaggagga cccagcccgc gacctcgttg agccgggcgc cgacgccgaa gaaccgccgg
    19021 ggctgcgtgg ccaaggtcgt cagtccttcc cgccttcacc gccggtttta ccggctacct
    19081 cgaagccgcg cagccgctcg tagaccttga ccacggcgct gtcgtcctcg cggccgtagc
    19141 cggcgccggc ggcggcgagg aactgctggt gggcggcggc cgcgatcggc agcgggaagc
    19201 gctgtcccct cgccgtatcg agcacgatgc cgagatcctt gacgaagatc tcgaccgcgg
    19261 aatgcggggt gaagtcccgg gccaggatgt gcggcacgcg attgctgaac atccaggaat
    19321 tgccggcgct gttggagatc acctcgaaga gcgtctgtgg gtcggcgccg caggcggcac
    19381 ccagcgccat ggcctcgccg gcggcggcga gatgcacgcc ggcgagatgc tggttgatca
    19441 tcttcacggt cgagccgatg ccgggctcgc tccccagccg atagacggtc ttcgccatcg
    19501 cgtcgagggc cggggccgcc gcggcgaagg ccgcctcgct gcccgacgcc atgacggtga
    19561 gcgtgccggc aacggcgccg acctggccgc cgctgatcgg cgcgtcgaga aggtcgaggc
    19621 ccatctcggc gagccgctcg cccagggact tcgtgaaggg tggcgggacg gtgctgtgca
    19681 gcaccacgac cccgccttca ccgagcacgg cggcaccacc atccttgccg aagagcgctg
    19741 tctcggcctg gtcggcggtg gcgacgacga ggatcaggag cgagacctgg tcggcggcgg
    19801 cgcgcgggct cgcgaccggc ttgccgccgg ccgcggccag ctcctcgagc ggcttcttcg
    19861 cgacgtcgaa gccgtagacg gtgtggccct tggcgagcag ctgcttcgcc atcggcaggc
    19921 ccatggagcc gagcccgacg aagccgatac tgatggtcat cccggttgtc ccaagcggcg
    19981 gtggcgctcg accggcaggc gttgccggac gagctggaga taggcggggt cgagctcggc
    20041 cgtggcatgg cccggccgat cggagaccct ggccacgaca tgcccccagg ggtcggcgac
    20101 gagcgcgtgg ccgtagctgt ggcggaggcc cgatccggcg gggaagctgc cgacctgggc
    20161 ggcggcggcg acatagcact gcgtctcgat ggcgcgcgcc cggagcagca cctcccagtg
    20221 gtccttgccg gtcggcaggg tgaaggcgga ggggacgagg atgagctcgg ccccggcccg
    20281 acgaagggcg agaaagagct cggcgaagcg gagatcgtag cagatggtga gcccgaccct
    20341 gaccccgtcg aggtcgaccg tcaccacccg gtcgcccggc acggtgtagt cgctctcgcg
    20401 atagacctcg ccggacgggg tctcgacgtc gaagaggtgg atcttgcggt agcgggcgac
    20461 ctcctcgccg gtgcggtcga tgaccactgt cgtgttggcg aggcggccct cgcccagatc
    20521 ctcgaagaag ctgccgccgt ggacgttgac gcggtgctcg cgggcgaggt cccgcagcat
    20581 ggtccaggcg gcgccaccag ggatcgcctc ggcggccgcg cgccggcccg cattgccacc
    20641 gcccatgatc gtgaaggtct ccggcaggct caccagatcc ggccggtcac gctcgatggc
    20701 tctttcgatc agcgccttcg cggccttgag gttcgccgcc ttgtccggcc ccgagttcat
    20761 ctggatcacg gcgacgcgca tgatggtcag gccccggcga cggcgaagcg atgcaccata
    20821 cggtgccggt aggtctcgtt cggccgcagg atcgagctcg ggaactccgg cttgttcggg
    20881 gcgtcgggga agcactcggt ctcgaggcag agaccggccc tgggaccgat atgcgccccg
    20941 tccgaggcgg tgatgtccat cttgaagccg gtgtagagct gcacgcccgg ctggtcggca
    21001 tcgagcgtca tcctgacccg gccgtcgctg gcgacgagat cggcgacggg gcgcaggccg
    21061 cttccgttca ccacgaagca atggtcgtag tcggcggtgc ccttggcgtc gatccgtcgg
    21121 gcttcgcgga agtcgaaccc ggttccggcc aacggccgga tctcgccggt cgggatctgg
    21181 ccctcgccgg tcggcaggta gccgtcggcg gcgagccgca gctcgtgcgc ctcgatcgag
    21241 cctttgccgt cgaggttcca gtaggtgtgg tgcaccatgt tgatcggcgt cggtgctgtg
    21301 gtcgtggcgg tcatctcgat gtcgaggctg tcgtcggtca gctggtagag gcagcgcgcc
    21361 tcgaccgtgc ccggatagcc cgcctcgccc gccggccccg tcagctccag ggtgaccgac
    21421 cggccgtcac cgcccggcgt catgcgccag agccgccggc cccagccctt cgagccgcca
    21481 tgcaggtggt ggacgccgcc catgttggct tcgagatcgt ggcgcacgcc gtcgagggtg
    21541 aaggtggcat ggccgatgcg attgccgaag cggccgaccg tggcgccgaa ataaggcgac
    21601 ttcgccgggt agtcggggaa gccgtcgaag cccagcacca acggacgcgg ccggccgtcg
    21661 acgtcgattg tgagggactg cagcacgcca ccccagctca ggatcgccgc gcgcaggccc
    21721 ttgccgttgt cgagcacgac ccggccgacc ggctcaccct cgaactcgcc ccatttctcg
    21781 accgccatga ccagtccccc tgccccttgc cgcagcctct atagcccatg ccgagcggca
    21841 ggtcagtcgc cgagaaggct gagccggccg agcccgggga gcgtgatctc caggtcgagc
    21901 gggttgatgc cgaggacgat gcccgagaag ttgagctcca gcccctcggc ccgggcgacc
    21961 atcaggccga aaaccccgcc gagcgagagc tggaagccgg tgtggctcgg ggccggggca
    22021 aggatgcggg ggccgagata gtccttgccg atggcgcggg gcggcaggtc cagcccgaga
    22081 cccggcacct cgcgggcgat ccaggcgatg aaggtgttgg agttcgggcc tggccacatg
    22141 cggtaggtgt gacgataggg atagtcggtc accgcctgct cgatctcggg aatgagcgct
    22201 gccgcagccg caccacggtg gtcgagcagg aggcgcggcc ggttgccggc ccagttgccg
    22261 tccggcacgc cccggtggtt gacccggatt gcctcgccgc cgcgccgcag atcccaggcg
    22321 accacgtcgt aacgggtgta gccggtcgcg ttctccggct tcatcgcgat ccagcaatga
    22381 tccgccacgg cccccttgag gcccagggag gcggcgcaat agacctgcac gaccgcttcg
    22441 gtcgcggtga ccgggtcggg tgccatgccg gtgctgtccc agcgcccgcc gccccagtgg
    22501 gccacggcac cctggcgcca ggcggagacg ccgaccggca ggatgacgag ggccagaacg
    22561 agcaggagcg gcgggcgcca gcgcaacggg cgagctttct tcggatgggt gcgtggttta
    22621 tgtagcgctt gcagggctca cgggcgaggc ttggaggcga agcatgacga aacgcgcacc
    22681 cccgggcagc catatggtcg ttatggccgg tctcgtcggc gccctcttgc cggccaccct
    22741 cgcggccgcg gagatggtcg agcgtggccg gatcatcgcc gagaccttct gcgcccaatg
    22801 ccacgccact ggcgccaccg gcgcgagccc gctccccggg gcgccgccct ttcgcacctt
    22861 caaggagcgc tggccggtcg aggtgctggc ggaggccctg gccgagggcc tgaccacggg
    22921 ccatccagag atgcccacgg tcaccatgac tccgggcgag atcgatgcgt tcctgggcta
    22981 tctcgacagc ttctgatcgg ccagcggctg cggcgattca cggcggagga tttgaatgtc
    23041 caggtcgatg agcgagaagc tggggctgat cgtcgaagac tcgctgagcg aaatctacgt
    23101 cttcaacgcc gacacgtacg agttcgtgct tgtgaaccgc ggcgcgcggg agaacctggg
    23161 ctacacgatg gatgagctgc gcgagctcac accctgggac ttgaagccgg agttcacgcg
    23221 cgaggccttc gtgcggatgg tggagccgct gcacgccggg gccgagcgat ttctgacgtt
    23281 cgagacccgg cacgagcgaa aggatggctc cgtctacgac gtggcagtga agctgcaatt
    23341 gatccgcggt gacgacgatg ttttttatgc ggctatagaa gatgtcaccg agcgaaagaa
    23401 agtaacggag gagcttcgcc aaacgaaccg gcggctggat gcgatcctga acaacaccaa
    23461 gatggcggtc ttcatgatgg acgaccgcca gcactgcgtt ttcatgaaca aggccgcgga
    23521 ggagctgacg ggctacactc tcgaggagac gcttggccgg ccgctgcacg acgtcattca
    23581 ccatagctat ccggacggtc gccctttccc gctcgcggag tgcgccatcg acagcgcttt
    23641 cccgcaaggc aaccagatgg aaggcgagga ggtcttcgtc cacaaggacg gcagcttcta
    23701 tcccgtcgcc ttcacggcct cgcccattcg cgacgatgcg gggcagcccg taggcacggt
    23761 catagaggtg cgcggcatcg ccgaggactt gcggacccgc gaggcgatgc gtgccttcaa
    23821 cgagacgctg cagcagcgcg tcgccgaggc ggtcgccgag cagcgcaagg cggagctgca
    23881 gctcgcccag gcccagaaga tggaagccgt gggcaagctc acgggcggcg tcgcgcacga
    23941 tttcaacaac ctcttgcagg tcatcggcgg cagcctccaa ctgctgacgc gcgatgtggc
    24001 gggcaacgaa cgcgccgagg gtctcgtgcg aaacgcgctc gtcggcgtca cgcggggcgc
    24061 caatttggcg tcgcaattgc tgtcgttcgg tcgccgtcag cccctcgagc cgcggccgat
    24121 caatcttggc cgcgtccttc gcaacctcga cgacatgctg cggcgcgcgc tcggcgagga
    24181 aatcgagatc gagaccgtgg tggccggcgg catctggaac tgcctggcag atgccaccca
    24241 ggtggagaac gccattctca atctggcgat caactcgcgc gacgccatgg gcggccgcgg
    24301 caagctgacg ctcgaggcgg gcaatgcctc gctcgacgac gcctatgccg ccgagcacgc
    24361 ggaagtgacg gccggccaat atgtcatgct cgccgtcacc gataccggca gcggcatccc
    24421 tgccgacgtc ctggagcgcg tgttcgaccc gttcttcacg accaagccgg aaggcgaggg
    24481 cagcgggctc ggtctcagcg gggtctacgg cttcgccaag caatcgggcg gccacgtcaa
    24541 gatctacagc gaggaaggtc aaggcacgac ggtgaggctc tatctgccgc ggacacgccg
    24601 gccggaggac agcgtgcccg agggcgttgc tgaggaggtc accggcggcg tcgagaccat
    24661 cctcgtcgtc gaggacgacg aggccgtgcg ggcgacggcg gcggagatgc tcaaggagct
    24721 cggctatcgg gtgttgaagg ccaaggacgc cgacagcgcg ctggccatcg tcgaaagcgg
    24781 catggcgatc gacctcctat tcacggatgt ggtgatgccg gggaagctgc ggagcacgga
    24841 gctggcggat cacttccgcc ggcgctcgcc gggcggtgcc gtgctgtaca cgtcgggcta
    24901 cacgcagaat gccatcgttc acgccggacg gttggacgac gaggtcgagc tgctgatcaa
    24961 gccctacggt cgcgaagcgc tggcccgcaa ggtgaggcag gtgctcgacg cgtcgtcggc
    25021 cacggcgcag ccgcccgccg ggaccgaggt cgagccccgc agccggcgca gcgtgctcgt
    25081 cgtcgaggac gaggtgctca tccgtatcaa caccgtcgac atgctgacgg agttgaattt
    25141 tctcgtcgcc gaggcggcaa cggctcggga agcaaggacc ttgctgggcc ggaagtcctt
    25201 cgacctgctg ctcacggacg tcgcccttcc cgatggctcg gggctcgagc tggcgcgcga
    25261 ggccgtggcg cggcacaagg agcttcgcgt ggtgctggcg accgggcagg tgatggaagc
    25321 gatggaggac ctgccgggcg cctcgtggct gcccaagcca tacgacgagc gggccctggc
    25381 cgtcgccgtc ggcacctggc cagcccggag cgacgggggc ggctcctagc ccagctcctc
    25441 ctgcggctgc tggttgccgc gcctgggggt ggcagcaccc accggtgccg cccagcggac
    25501 ggcgttcagg atcagcatct ggatctgggc gttgtggtag gtgggatagg tctcgtggcc
    25561 ggggcggaga taggcgatgc ggccctggcc gcgccgccag gtgcagcccg agcggaagac
    25621 ctcgccgccc cggaaccagc tcacgaggaa gagctcgtcg ggcggcggga tgtcgaaatg
    25681 ctcgccgtac atctcctcgt gctcgatgac gatctgctcg ggcaggccgg ccacgatggg
    25741 gtgggtgggg tcgaccac
//
LOCUS       DAOTFI010000027        25428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2260_length_25428_cov_7.438813, whole genome shotgun sequence.
ACCESSION   DAOTFI010000027 DAOTFI010000000
VERSION     DAOTFI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 25428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25428
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2260_length_25428_cov_7.438813"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="P5558_04210"
     CDS             complement(<1..290)
                     /locus_tag="P5558_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23546.1"
                     /translation="MTKRPIVDRAEVGIDFPDKSYLGSFTRHSAFEAAADHEGVTIRL
                     IRPGTESRQADIHLHYHLFADVLDELAGAIAAGHPVDEAHRGPLLAAARHLAA"
     gene            363..1169
                     /locus_tag="P5558_04215"
     CDS             363..1169
                     /locus_tag="P5558_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HRY23547.1"
                     /translation="MSELGVAIGGFGAIGREVAKALVAGLPGLRLAAVSGRDRAKAEA
                     GLAELGTGVPVLPLEALADAAEIVLECLPAAVFRDVAEPAIDQGRIFMPLSVGQLLTH
                     GDLIERAKATGARILPPTGALLGLDAVRAAAEGRIESVAMVTRKPPGGLAGAPHLVKN
                     GISVEGLTEPLMVFEGTAREGARGFPANVNVAAALSLAGIGPDRTTLQVWADPGVTRN
                     VHRIIVEADSARFEMLIENVPSANPKTGKITALSTIAALRGLTATLKVGS"
     gene            1228..2577
                     /locus_tag="P5558_04220"
     CDS             1228..2577
                     /locus_tag="P5558_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRY23548.1"
                     /translation="MLTEELSRTVAAPNNLEAFWMPFTANRHFKLEPRLIARAKDMHY
                     TSIDGRQILDATAGLWCCNAGHGRPRIVEAVQQQVADLDFAPNFQFSHPKAFELASRL
                     ATLFPGDLDHAFFTNSGSEAVDTALKIALAYQRARGQGTRTRLIGRERGYHGVGFGGI
                     SVGGMVKNRMWFGSLLAGVDHLPHTLDLERNAFSRGQPAHGAELADELERLVALHDAS
                     TIAAVIVEPVAGSIGVYPPPVGYLERLRAITEKHGILLIFDEVITGYGRLGKGSAAEY
                     FGVQPDLVTTAKGLTSGVIPMGAVMARKHIHDTIVGSSADERTIELFHGYTYSAHPVA
                     CAAGLATLETYREEGLFERAAELAPHWEAALHSLKGLPNVIDIRNIGLMGAVELSARP
                     GKPGARAYDAMCKGFFDHDIMIRITGDTLAMSPPLIVTKDQIDEIVERVGKVIRAVA"
     gene            2713..2916
                     /locus_tag="P5558_04225"
     CDS             2713..2916
                     /locus_tag="P5558_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23549.1"
                     /translation="MTSSLLTIVETSAYLARAEKLLDAEEREAVKLAFAAEPLAGDLI
                     RGSGGVRKLRFGLAGRASAVAHE"
     gene            3060..3359
                     /locus_tag="P5558_04230"
     CDS             3060..3359
                     /locus_tag="P5558_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY23550.1"
                     /translation="MTKTAFAAIEAGLADAIAHARGEPDRGTESRYPIGAVDVQAIRR
                     RTGLSQERFCALFGISRRTFVKWEQGERRPTGPARVLLKVIERNPTAVLEALKVD"
     gene            3521..5020
                     /locus_tag="P5558_04235"
     CDS             3521..5020
                     /locus_tag="P5558_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRY23551.1"
                     /translation="MRFANRLLAASCLVAGLFALPALAEPVSIKLLQLNDWYRYLDDE
                     GRGGFARLVTILADEDAAADNVLLVHAGDAFSPSLLSGFDQGAHMVALLNELPLDVFV
                     LGNHEFDFGPEVAAERLAEAGFDVVNSNVRTAGGDLFPGTVESKLVEIDGIKLGFYGV
                     TTPDTIELASPGDTTFAPVVETASLMQAKLREAGADIVVAVVHTGWREDMELYRAGIA
                     DIIMTGHDHDLRIMYDGQTVMTESGSDADYVTAIELLAERDEDGKVAWRPAFRAIDSA
                     RVEPDAAAQAKVQPYEDKLSAELDVDIGEAMVPLDTQRATIRTMESGFANLIADAIRN
                     ATGADIGLTNGGGIRGNRLYEPGHVLTRRDILSELPFGNTTVLVELQGSDLMAALENG
                     VSQVEEGAGRFPHVSGLRFTYDASQPAGARVTEVMVGDAPLDLAATYSVATNDYMLGG
                     GDGFASLGNGKVLIDGSGGTLMANTVMNYITATGGKVDQEAEGRVTRLN"
     gene            5169..5306
                     /locus_tag="P5558_04240"
     CDS             5169..5306
                     /locus_tag="P5558_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23552.1"
                     /translation="MRRGLMPFLVISLLTLGGLALSACAIQLPDGTQTEGWLKDRNKY
                     Q"
     gene            5329..5700
                     /locus_tag="P5558_04245"
     CDS             5329..5700
                     /locus_tag="P5558_04245"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRY23553.1"
                     /translation="MPSERGLRAAALVLWLAAWAVVTWAMLDPAPPDLAGQSDKLVHF
                     GSFFLISITSLGFCRGSGQLLAAGLVCLVAAVGLELTQGLVPSRTFEAADMAANLAGV
                     ATGMALARFALVRLGRRPSPA"
     gene            complement(5705..6073)
                     /locus_tag="P5558_04250"
     CDS             complement(5705..6073)
                     /locus_tag="P5558_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRY23554.1"
                     /translation="MSYDTSNVFARILRGELPCRKVYEDEWALAFEDIHPAAPVHVLV
                     IPKGPYVSLADFSAQAPDAAIAGFFRAVGKVADMAGVRESGYRVLANHGPDSHQEVPH
                     FHVHILAGRKLGPMLAPQHR"
     gene            6216..7187
                     /gene="gshB"
                     /locus_tag="P5558_04255"
     CDS             6216..7187
                     /gene="gshB"
                     /locus_tag="P5558_04255"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HRY23555.1"
                     /translation="MSLAVAIQMDPLETVDIDGDSTFVLALEAQRRGHGLYHYEPKHL
                     SYRQGRVLARARPFEVRREKGNHVTFGAPEVIDLATLDVVLMRQDPPFNLAYISATHL
                     LDRVHPGTLVVNDPHSVRNAPEKLFVTHFAELMPPTLISYDIDEMRAFRAEQGDVIVK
                     PLYGNGGAGIFLIKQDDNNLSSLVETFQSFLKEPLMVQRYLPEIRQGDKRIILIDGKP
                     AGAVNRVPPEWDVRANLHIGGRAEKAVLTKRDLEICEVIGPALEEYGLVFTGIDVIGD
                     YLTEINVTSPTGLQEIDRFDGVSLERQIWDAIETRLANGPTARRWMS"
     gene            complement(7388..7891)
                     /locus_tag="P5558_04260"
     CDS             complement(7388..7891)
                     /locus_tag="P5558_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRY23556.1"
                     /translation="MARDLAYDAGDYIVYPSHGVGRITGIEKQTIAGMHLEMYVVSFE
                     NEKLILRVPVEKAPNSGMRKLSSREKIQSALDALKIRTRPRRNIMWSRRAQEYEAKIN
                     SGDPVSIAEVVRELHRADDTDQSYSERQMYRAALERLAREVAAIDSVDETEATRKLEK
                     VLLAAAA"
     gene            complement(8199..8540)
                     /locus_tag="P5558_04265"
     CDS             complement(8199..8540)
                     /locus_tag="P5558_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRY23557.1"
                     /translation="MTYVVTEACIRCKYMDCVEVCPVDCFYEGETMLVINPDECIDCG
                     VCEPECPAEAILPDTESEAEKWLELNRQYSTGPWPNITRKGEEPADADEHKDEKGKYE
                     KYFSPNPGSGS"
     gene            complement(8627..10462)
                     /gene="mutL"
                     /locus_tag="P5558_04270"
     CDS             complement(8627..10462)
                     /gene="mutL"
                     /locus_tag="P5558_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRY23558.1"
                     /translation="MAARAESSAPTARGPAPRIRRLPPGLVNRIAAGEVVERPASAVK
                     ELVENAIDAGATRVRVTLEEAGRRAITVEDDGSGMTRAELELAVERHATSKLLDEQLV
                     DIRTLGFRGEALPSIASVSRFTLTSRTASEAHGWRLAGDGGRLGAAEPAAAPAGTRVD
                     VLDLFFNTPARLKFLKSERRELELAREIVERLALAHPKTGFRLEAGGRRLLDLAPADR
                     EARLTALLGRPVVENSIEIAAERDGLRLFGLAALPTLSRNHGRDQHLVVNGRPVQDRL
                     LKGALRAAYADLLPANRQPIAALFLELPTALVDVNVHPTKAEVRFRDSGEVRGLIVGA
                     LKRALAEHGHRSSTTTGAAALARFTPGGNLGGGPGAGSGAWSRPEVPPAAGLAEAAQR
                     YQAPPGLDIGPPSMRHGDAPPPEGAAEFPLGAACAQVHETYIVTQTRDGIVIVDQHAA
                     HERLVYERLKKALAAGAVARQTLLIPEVVTVDGRTRELLLERAADLVPLGLVLEAFGS
                     EAVLVRETPAPIGTDSVASLLHDIAGDLASLDQALPLEEALLKVVARAACHGSVRAGR
                     RLTPAEMNALLRDMEKTPHSGQCIHGRPTSIALGKADIERLFGRR"
     gene            complement(10446..11777)
                     /locus_tag="P5558_04275"
     CDS             complement(10446..11777)
                     /locus_tag="P5558_04275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY23559.1"
                     /translation="MPHTMPATTTAFGTDVERVLTPGGLEAWLVNAPSVPLLAVAFSF
                     RGGAAADPAGSEGLSRMVAGLLDEGAGELDSLAFRTELEDLAIRLQLTAERDALAGRL
                     MTLNGNRERAFELLRLALAEPRFDAEPIERIRQQITSDLQRRRTDPNTVAGRVWFAHA
                     FPDHAYGRPAQGTPEAIRAVTRADLETYVASRLARDNLVIGVAGDITAAELAPLLDRA
                     FAHLPALADLPPLLPATPATGATLVTPMTIPQSVVTFGLPGIPRDDPDYYAAYVANYI
                     IGGGGFASRLLEEVREKRGLAYSVYSHLVDAELAPLWMGGVATRNDQVAQSIEVIRAE
                     LGRMAEGDIGETDLANARTYLTGSFPLRFTSNESVARMLVGMQVADLGIDYLERRNAL
                     IEAVALADVARASERLFSAEPLFSVVGDPVGLDGATRLSPLVAANGNGGKG"
     gene            complement(11784..13181)
                     /locus_tag="P5558_04280"
     CDS             complement(11784..13181)
                     /locus_tag="P5558_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY23560.1"
                     /translation="MPEAGAMATGLNLAAEAGAPMGMFHPQTFTLDNGMQVVVIENHR
                     APVVSHWVWYKVGTADSPPGKSGLPHFLEHLMFKGTEAIAPGEFSKIVARHGGNDNAM
                     TSSDFTAYFQNIARDRLAMVMEMEADRMANLRLDAAHVLAERDVILEERSSRVDNQPG
                     AMLHEQLDAAQFLNHPYRLPIIGWHHEIASYTREDALAFYDLWYAPNNAILVVAGDIT
                     MDELRPLAERTYGRVAPRPTPERRRLVEPPQLAERRIELAHERVEQPSLVRSYLAPSH
                     ATAADGRAHALEVLAELFGGGGTSRLYRAIVVDQALASGIGAYYRGTSLDQTAFRIYG
                     SPRGEVDLDRLEKALDAEIERLVEDGVTEAEVERVKARLIAESVYARDSLTTAVRVFG
                     SALTTGSTIEDVESWPASIAAVTRPAVEAAARELFDRGRSVTGRLTPARNGNGKRPAG
                     DAATADAATAAAATG"
     gene            complement(13195..13758)
                     /locus_tag="P5558_04285"
     CDS             complement(13195..13758)
                     /locus_tag="P5558_04285"
                     /inference="COORDINATES: protein motif:HMM:NF022679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HRY23561.1"
                     /translation="MKRAGQGSRIAFGCMALVLVALTGCGSGTLQERLGMGRRAPDEF
                     QVVRRQPLVLPPSYTLPKPGTPSPAQQQGTASLQTQELLFGQPVPEADPSPAEEALLA
                     GLPGTVEPNIREVILEENTEMTQLDESRFLFILDFQRRRMVDSSGVYQPLDPTAEAAR
                     LEAEGQSARVVTQRVGTSVVEPPAPEQ"
     gene            complement(13755..14258)
                     /gene="lspA"
                     /locus_tag="P5558_04290"
     CDS             complement(13755..14258)
                     /gene="lspA"
                     /locus_tag="P5558_04290"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRY23562.1"
                     /translation="MRNDPARRWLGPGVFLLILVLDFLTKQWALASLADRGFVEVTGF
                     FNLVLVWNRGVSFGMFQAGSEAGRWLLVGVALLVSALLVVWLRRERRKLPRLAIWLIL
                     AGALGNVVDRVRYGAVVDFLDFHAFGYHWPAFNVADSAIVVGAALLVGDSLLGGREAL
                     PVAGRQK"
     gene            complement(14255..17047)
                     /gene="ileS"
                     /locus_tag="P5558_04295"
     CDS             complement(14255..17047)
                     /gene="ileS"
                     /locus_tag="P5558_04295"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRY23563.1"
                     /translation="MTKDYPKDYRDTVFLPRTDFAMKANLPAREPAQLERWAAEAQYG
                     RLREAARGRERFILHDGPPYANGHLHMGTALNKILKDVVNRSRQMLGKDAAYVPGWDC
                     HGLPIEWQIEQEYRKRGKNKDDVPIIDFRRECREYAARWIDIQRAEFERLGVVGDWDR
                     RYATMDFRSEADIYRELAKFLLNGSLYRGKRSVMWSVVEKTALAEAEVEYHDHTSTTI
                     WVAFPLIRPAQPRFADAAVVIWTTTPWTIPANRAVAYGAAIDYALVAAAGRRLIMAET
                     LVEATMATAGIADWQVLETFKGTALAGSIAAHPLAGHAGGYGFEVPLHPADFVSDEDG
                     TGLVHIAPGHGADDFELGRRHGLEVPDTVAEDGSYTAAVPGFEGIHVFKAAEPVVEAL
                     RAEGNLLAQGRLVHSYPHSWRSKAPLIFRATAQWFIPMEGELDLRQKALDAIDATTFV
                     PARGKNRLRSMIETRPDWCISRQRAWGVPIAVFVDKKTGEPLRDPAVIERIALAFEAE
                     GADAWWTRDPQDFLGNDHRKEDFEKVDDILDVWFESGSTHAFVLEQRPELKWPADLYL
                     EGSDQHRGWFHSSLLESCGTRGRAPYDAVLTHGFVVDAKGEKMSKSKGNVLSPIDLMK
                     THGADILRLWTVSADYAEDIRIGKEILDGIGDNYRRLRNTLRYALGNLAGFREVERLP
                     CDRMPELERWVLHRLAELDDLVRSCNASYDFPRLYGAIHHFCAIDLSAFYFDVRKDSL
                     YCDAVDSVRRRAARTVLDRVFNCLVRWLAPVLVFTAEEAWLARGQGETDSIHLELFET
                     LPGDWRDPALAARWEKVRAVRRVVTGALEIERREKRIGASLQAAPVVHVTEAHAAAIE
                     GLDMAGLCITSGIEIVVGTPPGGAFTLADVPGVGVVPALAAGEKCGRCWQVLEEVDEA
                     GGLCIRCTGAVGAMAA"
     gene            complement(17093..18070)
                     /locus_tag="P5558_04300"
     CDS             complement(17093..18070)
                     /locus_tag="P5558_04300"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRY23564.1"
                     /translation="MRVHRGFAALPPEAVGCAVAIGNFDGVHQGHRAVIEAARRQAAH
                     GQAAHGQVARAGGLGVVTFEPHPREVLSPATAPLRLAPLRRKIALLRDLGVDHLFLLR
                     FDRALMAMPAPEFVERILVGRLGIRGLAAGEAFRFGHRRQGDAALLQEAAAAHDFAFA
                     AVPPVMADGLPCSSTRIRVALAAGELALANRLLGYPFELTGIVGQGHRRGRTIGFPTA
                     NIAPAAERYALPASGVYAVRAGRCTPAGKVWYPGIANLGRRPTFDGDRLLLEVHLLDA
                     RLDLYGQRLDVAFLERLRGEVKFPGIEALKAQIAEDCSAARRIHDRLDA"
     gene            complement(18176..19618)
                     /locus_tag="P5558_04305"
     CDS             complement(18176..19618)
                     /locus_tag="P5558_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23565.1"
                     /translation="MPPFLRILALGTIVAAAAVLQPTLAPLALAAEPFSFRNSFQLGL
                     PGRQATAGDAIPKLKLGQQRLLPGFPALTLPGSGIATSGGTTSGGSTSGGSTSGGSTA
                     GGATAGNPTAGGATSGGSTAGSPASGGSGSSSPTDVPAIPSPAANLVIHYVQPSGFQK
                     VTGRADQDLLLVSPNQSINGKFQIVVEGFRGVYWIGASFDPQPAGWLTSPNGKRTEGV
                     GTLVRIRTHKDAVRPFIVLGRMNLKTSSIDFGDFAQLGGDTAAGKWQAWPDVWRCRII
                     AEPLFGWRDYKGGSFSRYVSHSDFTKFETGGVRHSYAARIDATWGYQGEYVLPSYASD
                     KKPYRGPDGRGTAQYWDYVARAVTNSAIGSESKPKAFFLARSSEEVAAGDYITYRFNT
                     SGRGVWVVPVKGRPVTDHIHPGGGAYAPQVTGSTLTWRNPSGDPFVVGRINDGSKATP
                     PTVVGTGEVGYRQRVTDRPGLERAIASLCR"
     gene            20244..22733
                     /gene="gspD"
                     /locus_tag="P5558_04310"
     CDS             20244..22733
                     /gene="gspD"
                     /locus_tag="P5558_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HRY23566.1"
                     /translation="MRTVDFDPPPGGETAARSPTPTTGGTATGERPQSQLFFGQNPVP
                     GAETAAAADTTGGVRLNFDGASLREVVDVVLGEILGVSYTIDPAVSGDVILSSTAPLS
                     RADMLTVLETILQMHGATLVDLGGTYAVMAGAPTVGAAQFTTVGGEQAPPLIVGTGVT
                     VVPLRYVSAAAASQFIQPLLSLPEQIRIDDARNLLLFVGNAAERQNVVRTLGEIDVDW
                     MAGRSVGIFPLSTATPESVIAELDGLFSPFGGFDINTSGVRFLPMDRLNAVLAITSQP
                     EQLVDIERWVQRLDRGSGSSVQFFVYQLQNTPALPMAELLNATFADAAAADLVPASAG
                     LAGEFGAVPSQAGLETGDFLGDTAPLPFDAPFGDLPPSQRRDVLSSVKVVPNELSNSL
                     VVRATPQAYALIEQTIRRLDVAPLQVLIEATIAEVVLNDQLRYGVQYFFQAGSFRAGF
                     NTTTDGGASGAVNPNLLDPLALVPGFNFVYSGASSNITIDALSRITDVQVLSSPSLVV
                     QDNSEAVLTVGDEVPLVTRTSQGVENPDSPIVANIEYRETGVILEVKPRINFNNIVSL
                     QISQEVSRVQPVGEDQFGNPTIAQRRITSSVNVTSGQTVVLGGLIQDDTIRNNSSVPG
                     LGDIPVLGNLFGSTNDVSQRTELIVFITPRVIRNADDARAVSEELRDRMRAAQPPADS
                     AGEPLTPAGAPPPVPTTTPPAVPEPAPPPAPPATTITPRPLTTSELPEPALPDPEPAG
                     APAEAPGEEPVPGIVPVAWVEPTAVVPPVALPVVEPPSVGPPDGEPPRRRPSVDPAAA
                     DAPPANLPLARPIPGDVVLPAPRPGEVGIAL"
     gene            22772..23512
                     /locus_tag="P5558_04315"
     CDS             22772..23512
                     /locus_tag="P5558_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRY23567.1"
                     /translation="MAAALVGLAVGSFLGALASRWPALDRGFLFGRSRCAACGAGLGW
                     RETLPLWSWVAQRGRCRHCGGAIGWRPLAAELAGAAIAGTAFAALAPAAALLLTGVGW
                     CLLLLALIDAAHGRLPDILTLPLALAGPLVAALAPLPGLVPPLEGLLGAGLGFALFWA
                     IGRLYAAWRGQEGLGLGDAKLLAAAGAWLGPPGLAPTILVAAITGLVAAWLGGRRRGG
                     DAIAFGPFLALACWLLLLRQLAGSQGGS"
     gene            complement(23509..23994)
                     /locus_tag="P5558_04320"
     CDS             complement(23509..23994)
                     /locus_tag="P5558_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HRY23568.1"
                     /translation="MIIPASRSSPHDRLPPMTTFRKVASDFYVAPQLEPADFAAAAEL
                     GIRTVINNRPDGEAPDQLADAEARALAAAQGLAYVHVPVVSGGILPDHLAAMRAAIDG
                     HQGPFVAYCRSGTRSCHLWALVEARDEDPDALIAAAATAGYDLGAIRPLLKEIHKKAG
                     A"
     gene            complement(24833..25168)
                     /locus_tag="P5558_04325"
     CDS             complement(24833..25168)
                     /locus_tag="P5558_04325"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRY23569.1"
                     /translation="MVRREAFEAAEARAAGLMAAGPIAFAARDTEGLERARPADLEPI
                     QISRSGLGLHFPRLDADLHLPALLEGVFGSRRRLLARAEEPVAEQPRRNAPGGGDLDR
                     LAAITNAYT"
BASE COUNT         3615 a         8655 c         9194 g         3964 t
ORIGIN      
        1 gcggccaggt gccgcgcggc tgccagcagc gggccgcgat gggcctcgtc gacggggtgg
       61 ccggcggcga tggcgccggc gagctcgtcg agcacgtcgg cgaagaggtg gtagtggagg
      121 tggatgtcgg cctgccggct ctcggtgccg gggcggatca ggcggatcgt cacgccctcg
      181 tggtcggccg ccgcctcgaa ggcggaatgg cgggtgaacg agccgagata ggacttgtcc
      241 gggaagtcga tgccgacctc ggcgcggtcg acgatcggtc gcttggtcat ggctctggct
      301 cctgcccctt gctctggtcg tgcagcgccg ggtagcaagg cggcgcagcg ggaggaagcg
      361 cgatgagcga gctcggggtg gcgatcggcg gatttggggc cattggccgc gaggtggcga
      421 aggccctggt cgccggcctg ccgggcctgc ggctcgccgc ggtgagcggg cgcgaccggg
      481 cgaaggccga ggccggcctc gccgagctcg gcacgggcgt gccggtgctg ccgctcgagg
      541 cgctggccga cgcggccgag atcgttctcg aatgcctgcc ggcagcggtc tttcgcgatg
      601 tggcggagcc ggcgatcgac caggggcgga tcttcatgcc gctctcggtc ggccagcttc
      661 tgacacatgg cgacctgatc gagcgggcga aggccacggg tgcgcgcatc ctgccgccga
      721 cgggcgcgct tctcggcctc gatgcggtgc gggccgccgc cgagggccgg atcgagagcg
      781 tcgccatggt gacgcgaaag ccgcccggcg gtctggcggg agcaccgcat ctggtgaaga
      841 acggcatctc ggtcgagggg ctgaccgagc cgctcatggt cttcgagggc acggcccgcg
      901 agggcgcaag gggctttccg gcgaacgtca atgtcgcggc ggcgctcagc ctcgccggga
      961 tcggcccgga tcgcacgacg ctgcaggtct gggccgaccc cggggtgacc cgcaacgtgc
     1021 accggatcat cgtcgaggcg gacagcgcgc gcttcgagat gctgatcgag aacgtcccgt
     1081 cggccaaccc gaagaccggc aagatcacgg cgctcagcac catcgcggcg ctgcgcggcc
     1141 tcacggccac gttgaaggtc ggctcctgat tgacttgccc cgcccggtgg cgggataact
     1201 cgcctgaacc gttgcaggag tgtcgtcatg ctgaccgagg agctgtcccg gaccgtcgcc
     1261 gcgccgaaca atctcgaggc cttctggatg ccgttcaccg cgaaccggca tttcaagctc
     1321 gagccgcggc tgatcgcccg cgccaaggac atgcactaca cctccatcga cggacggcag
     1381 atcctcgacg ccacggcggg tctctggtgc tgcaatgccg gccacggccg gccgaggatc
     1441 gtcgaggcgg tgcagcagca ggttgccgat ctcgacttcg cgccgaactt ccagttctcg
     1501 catcccaagg cgttcgagct ggcctcgcgc ctggccacgc tcttcccggg tgatctcgac
     1561 cacgccttct tcaccaattc gggctccgag gcggtcgaca cggcgctgaa gatcgcgctc
     1621 gcctaccagc gggcacgggg ccagggcacg cggacccggc tgatcggccg cgagcgcggc
     1681 tatcacggcg tcggcttcgg cggcatctcg gtcggcggca tggtcaagaa ccgcatgtgg
     1741 ttcggctcgc tgctcgccgg cgtcgaccac ctgccgcaca cgctcgacct cgagcggaac
     1801 gccttctcca ggggccagcc ggcgcacggc gccgagctcg ccgacgagct cgagcggctg
     1861 gtggcgctgc acgacgcctc gaccatcgcc gccgtcatcg tcgagccggt ggcgggctcg
     1921 atcggcgtct acccgccgcc ggtgggctat ctcgagcggc tgcgggcgat caccgagaag
     1981 cacggcatcc tgctcatctt cgacgaggtg atcaccggct acggccggct gggcaagggc
     2041 tcggccgccg agtatttcgg cgtccagccg gatctcgtga cgaccgccaa gggcctcacc
     2101 tcgggcgtca tcccgatggg ggcggtgatg gcgcgaaagc acatccacga caccatcgtc
     2161 ggcagcagcg ccgacgagcg cacgatcgag ctcttccacg gctacaccta ctcggcccat
     2221 ccggtcgcct gcgccgccgg cctcgccacg ctcgagacct atcgcgagga gggcctgttc
     2281 gagcgcgccg ccgagctggc gccgcactgg gaggcggcgc tgcacagcct caagggcctg
     2341 cccaacgtca tcgacatccg caatatcggc ctgatggggg cggtcgagct ctcggcgcgg
     2401 cccggcaagc cgggggccag ggcctatgac gccatgtgca agggcttctt cgaccacgac
     2461 atcatgatcc gcatcaccgg cgacaccctc gccatgtcgc cgcctttgat cgtgacgaag
     2521 gaccagatcg acgagatcgt cgagcgggtg gggaaggtga ttcgcgcggt ggcgtgatcc
     2581 tcgcgccctg gcgcgaccga caccttccga ccggctatcg gagtatctgg accagcgtgg
     2641 cggcgattcg agcgctgtag acgtctcgat gaggctggaa gttgcgtttt atgccccatt
     2701 ggggtattat cgttgacgtc atctctgctg accatcgtcg agacgtccgc ctatctggcg
     2761 cgagccgaga agctgctcga tgcggaggag cgggaggccg tgaagcttgc gttcgcggcg
     2821 gagccgcttg ctggtgacct gatccgaggc agcggtggcg tgcgcaagct gcggttcggg
     2881 ctcgccgggc gggcaagcgc ggtggcgcac gagtaatcta cttcttccat gataatccga
     2941 tgcccgcctt cctgctcact gtcttcgcca agaatgaacg cagcgatctg acgccgagcg
     3001 agatcgccac cctgcgcaag ctggttcgga cgctcgtcga gacctacgga gcctgagaaa
     3061 tgacgaagac agccttcgcg gcgatcgagg cgggacttgc ggatgcgatc gcccatgccc
     3121 gtggcgagcc cgaccgtggc accgagagcc gctatccgat cggcgccgtc gacgtgcagg
     3181 cgatccgccg gcggacgggc ctctcgcagg agcgcttctg cgcgctcttc ggcatcagcc
     3241 ggcggacctt cgtcaaatgg gagcagggcg agcgacgccc gaccggcccg gcccgggtgc
     3301 tgctcaaggt gatcgagcgg aacccgactg ccgttctcga agcgctgaag gtcgattgaa
     3361 ccgcatcggt cgtttcgaga gctttcatct ggccgggcaa cgacggtggc cgaccgaatc
     3421 gctggcggtt gctcaggcaa tgagtggccc gccgatagcg gctgacagcc tgcgccatgc
     3481 tgctatatcc gattgagcgg tcgtcaaggg aggaacggac atgaggtttg ccaatcgtct
     3541 gctcgctgcg agctgtctcg tcgccgggct gttcgccctg ccggcgctgg cggagccggt
     3601 cagcatcaag ctcttgcagc tcaacgactg gtaccgctac ctggacgacg aggggcgggg
     3661 cggcttcgcc cggctggtca cgatcctcgc ggacgaggat gcggccgccg acaatgtcct
     3721 cctcgtccat gccggcgacg ccttctcgcc ctcgctgctc tccggcttcg atcagggtgc
     3781 ccacatggtg gcgctcctga acgagctgcc gctcgacgtc ttcgtcctgg gcaatcacga
     3841 gttcgacttc ggccccgagg tcgcggccga gcgtctggcc gaggccgggt tcgacgtcgt
     3901 caacagcaat gtgcgcaccg cgggcggcga cctcttcccc ggcaccgtcg agagcaagct
     3961 ggtcgagatc gacggcatca agctcggctt ctacggcgtc accacgcccg acacgatcga
     4021 gctggcgagc ccgggcgaca cgaccttcgc gccggtggtc gagaccgcca gcctgatgca
     4081 ggcgaagctg cgcgaggcgg gcgccgacat cgtggtcgcg gtggttcata ccggctggcg
     4141 cgaggacatg gagctctacc gggccgggat cgccgacatc atcatgaccg gccacgatca
     4201 cgacctgcgg atcatgtacg acggccagac ggtgatgacg gagagcggct cggatgccga
     4261 ctacgtcacg gcgatcgagc tcctcgccga gcgcgacgag gacggcaagg tggcgtggcg
     4321 gccggccttt cgggcgatcg acagcgcccg cgtcgagccc gacgcggcag cgcaggccaa
     4381 ggtgcagccc tatgaggaca agctctcggc cgagctcgac gtcgatatcg gcgaggcgat
     4441 ggtgccgctc gacacgcagc gggcgaccat ccgcacgatg gagagcggtt tcgccaatct
     4501 catcgccgat gcgatcagga acgccacggg cgccgatatc gggctcacca atggcggcgg
     4561 catccgcggc aaccggctct acgagccggg ccatgtgctc acccggcggg acatcctgag
     4621 cgagctgcca ttcggcaaca cgacggtcct ggtcgagctc cagggatccg atctcatggc
     4681 ggcgctcgag aacggcgtca gccaggtcga ggagggggcc gggcgcttcc cgcacgtctc
     4741 ggggctgcgc ttcacctatg atgccagcca gccggccggc gcccgggtca ccgaggtcat
     4801 ggtcggcgat gcaccgctcg atctggcggc gacctattcg gtcgccacca acgactacat
     4861 gctcggcggc ggtgacggct tcgccagcct cggcaatggc aaggtgctga tcgacggctc
     4921 cggcggaacg ctgatggcga acaccgtgat gaactacatc acggccacgg gcggcaaggt
     4981 cgatcaggag gccgaggggc gggtcacgcg tctcaattga ggcgggaagc ggtcgtttcg
     5041 ccgcgcgcgg caaggcgggc cgattgacct gtggccgatc ggcccgctag tgatcgtcgc
     5101 tgcagcgcca tgcgccgctg gccgcaagcg ggcgtgtcgc cgccgggcct gtcgaaagga
     5161 agtagccgat gcgtcgcgga ctgatgccgt tcctcgtgat ctccctgctc accctgggcg
     5221 gcctggcgct cagcgcctgc gccatccagc tccccgacgg cacccagacc gagggctggc
     5281 tgaaggaccg caacaagtac caataggcgc tgcggggctc gccgctcgat gccgtcggag
     5341 cgcggcctgc gcgccgcggc gctggttctc tggctcgctg cctgggcggt ggtcacctgg
     5401 gcgatgctcg acccggcgcc accggacctg gcggggcagt cggacaagct cgtccatttc
     5461 ggcagctttt tcctgatcag catcacctcg ctgggtttct gccgcggcag cggccagctt
     5521 cttgcggccg gcctcgtctg tctcgttgcc gcagtcggcc tggagctgac ccaggggctg
     5581 gtgccgagcc ggaccttcga ggccgccgac atggccgcca acctcgccgg cgttgccacc
     5641 ggcatggcgc tggcccggtt cgcgctggtt cggctcgggc ggcgcccgag tccggcctga
     5701 cgggctaccg gtgctgcggt gcgagcatcg gcccgagctt gcggccggcc aggatgtgga
     5761 cgtggaagtg cggcacttcc tggtgtgaat cggggccgtg attggcgagc acgcggtagc
     5821 cgctctccct gacgccggcc atgtccgcga ccttgcccac ggcgcggaag aagccggcga
     5881 tggccgcgtc gggggcctgg gcgctgaagt cggcgagcga gacatagggg cccttgggga
     5941 tcaccaggac atgcaccggt gccgccggat ggatgtcctc gaaggccagc gcccactcgt
     6001 cctcgtagac cttcctgcag ggcagctcgc cgcgcaggat gcgggcgaag acattgctgg
     6061 tgtcgtagct catcggggat gctccagggg cggcggtcgc ctgtgcgtga tcgtgctcga
     6121 gggttcgtgg gcgggttggc cgcgacttgt caaggcgggc cgggactggc agagacggat
     6181 ggccgaatct tcggccctcc attcgggacg ccctcatgtc gctcgccgtc gccatccaga
     6241 tggacccgct cgagaccgtc gacatcgacg gcgacagcac gttcgtgctg gcgctcgagg
     6301 cgcagcggcg gggacacggc ctctaccact acgagccgaa gcatctgagc taccggcagg
     6361 gccgggtcct ggccagggcg cggccgttcg aggtgcggcg ggagaagggc aaccatgtca
     6421 ccttcggtgc gcccgaggtg atcgatctgg ccaccctcga cgtcgtcctg atgcgccagg
     6481 acccgccctt caacctcgcc tatatctcgg ccacgcatct gctggatcgc gtgcaccccg
     6541 gcacgctcgt cgtcaacgac ccgcattcgg tgaggaacgc gcccgagaag ctcttcgtca
     6601 cccatttcgc cgagctcatg ccgccgaccc tgatctccta cgacatcgac gagatgcggg
     6661 cgttccgcgc cgagcaaggc gacgtcatcg tcaagccgct ctacggcaat ggcggggccg
     6721 gcatcttcct catcaagcag gacgacaaca acctctcgag cctggtcgag accttccaga
     6781 gcttcctcaa ggagcccttg atggtgcagc gctacttgcc ggagatccgc cagggcgaca
     6841 agcgcatcat cctgatcgac ggcaagccgg ccggggccgt caaccgggtg ccgcccgagt
     6901 gggacgtgcg ggccaacctg catatcggcg gccgggccga gaaggcggtg ctgaccaagc
     6961 gcgatctcga gatctgcgag gtgatcgggc cggcgctcga ggagtatggc ctcgtcttca
     7021 ccggcatcga cgtgatcggc gactacctga ccgagatcaa cgtcacctcg ccgaccggcc
     7081 tgcaggagat cgaccgcttc gacggcgtca gcctcgagcg gcagatctgg gacgcgatcg
     7141 agacgcggct ggcgaacggg ccgacggcac ggcgctggat gagctgagcg cctgcacggc
     7201 gaccgcatac ttcgtcaggc tgcggtcgat cggtgctcac gtagcatcgg ctacgctgcg
     7261 cgccgatcgc cttgccttcc tcgtctacgg tcgccgtgca ggcgctcaga ccggtggcgg
     7321 ggccgagcgc caagcaaaaa gggctccctt gcgggagccc cagtcgccgg agtcgtgaga
     7381 gggcagctca ggcggcagcc gccagcagca ccttctcgag cttcctggtc gcctccgtct
     7441 cgtcgaccga atcgatcgcc gcgacctcgc gggccaggcg ctcgagggcg gcccggtaca
     7501 tctgccgctc gctgtaggac tggtcggtgt catcggcgcg gtgcagctcg cgcaccacct
     7561 cggcgatgga gaccgggtcg ccggagttga tcttcgcctc gtattcctgt gcgcgtcggc
     7621 tccacatgat gttgcgccgc ggccgggtac ggatcttgag tgcatcgagg gccgactgga
     7681 tcttctcgcg gctgctgagc ttgcgcatac cggagttcgg cgccttctcg accggcacgc
     7741 gcaggatcag cttctcgttc tcgaacgaga cgacgtacat ctcgagatgc attccggcga
     7801 tcgtctgctt ctcgataccg gtgatccgtc cgaccccgtg gctcggatag acgatgtaat
     7861 ccccggcatc gtaggcgagg tctcttgcca tcgacttgat tctccactaa cggtggtgcc
     7921 ccagccgcgc gaattcaatc atccctcgtt cagccgccaa ttcgaacgag cggcatcgcc
     7981 acacgaggca attcggtggc gtgacgcgtg cgtccatgcg gtccggccga aggcgatttt
     8041 cgaggttgtt cttcctcgct atccaacgca gcgtgtgatg tttagcacaa attcgtctca
     8101 aatgccacac tgctgttgca gtgcggcgag agcgcgcatc cgttggccgg tcgtcgtcgg
     8161 cgtttcccag tcgttcgcgg ccggctggtg cccggttgct agctgccgct gccgggattg
     8221 gggctgaagt acttttcgta cttgcccttc tcgtccttgt gctcgtcggc gtcggcgggc
     8281 tcctcgccct tgcgggtgat gttcggccat ggcccggtcg aatactggcg gttgagctcc
     8341 agccacttct ccgcctcgct ctcggtatcg ggcaggatgg cctcggccgg gcattcgggc
     8401 tcgcacacgc cgcaatcgat gcattcgtcg ggattgatga ccagcatcgt ctcgccctcg
     8461 tagaagcagt ccacggggca gacttcgacg caatccatgt acttgcacct gatgcaggct
     8521 tcggtgacga cgtaggtcat cggggctccg tccttcgcgc tggcggttcc ggttctagcc
     8581 aagaacggcg cgccgggcca gccggttcgg cacgttttcg cgcccgtcag cgacggccga
     8641 acaggcgctc gatatccgcc ttgccgaggg cgatcgaggt cggccggccg tggatgcact
     8701 ggccggaatg cggtgttttc tccatgtcgc ggagcagcgc gttcatctcg gccggggtga
     8761 gccggcggcc ggcgcgcacg ctgccgtggc aggcggcccg ggcgaccacc ttcagaagtg
     8821 cctcctcgag cggcagcgcc tgatcgaggc tggccaggtc gccggcgatg tcgtgcagca
     8881 gcgaggccac ggagtccgtg ccgatcggcg ccggggtctc gcggaccagg accgcctcgc
     8941 tgccgaaggc ctcgagcacg aggccgagcg gcacgaggtc ggccgcccgc tcgaggagca
     9001 gctcccgcgt ccgcccatcg accgtcacca cctcggggat cagcagggtc tggcgggcca
     9061 cagcaccggc ggcgagcgcc ttcttcagcc gctcgtagac cagccgctcg tgggcggcgt
     9121 gctggtcgac gatgacgatg ccgtccctgg tctgcgtcac gatgtaggtt tcgtgcacct
     9181 gggcgcaggc ggcgccgagc gggaactcgg cagcgccctc gggcggtggc gcgtcgccat
     9241 gccgcatgct gggcgggccg atgtcgaggc cgggcggcgc ctggtagcgc tgcgccgcct
     9301 cggcgaggcc ggcggccggt gggacctccg gccggctcca ggccccgctg ccggccccgg
     9361 ggccaccccc gaggttgccg ccgggcgtga acctggcgag ggcggcggcg ccggtggtgg
     9421 tgctgctgcg gtggccgtgc tcggccagcg cgcgcttgag ggcgccgacg atgagcccgc
     9481 gcacctcgcc gctgtcccgg aaccgcacct cggccttggt ggggtggacg ttcacgtcga
     9541 ccagagccgt cggcagctcg aggaagaggg cggcgatcgg ctggcggttg gccggcagga
     9601 ggtcggcata ggcggcccgc agcgcaccct tgagcagccg gtcctgcacc ggccggccgt
     9661 tgaccacgag atgctgatcg cggccgtgat tgcggctcag cgtcggcagg gcggcgaggc
     9721 cgaagagccg caggccgtcg cgctcggccg cgatctcgat cgagttctcg accacgggcc
     9781 ggccgaggag cgcggtgagg cgggcttcgc ggtcggccgg cgccaggtcg aggaggcgcc
     9841 gcccgccggc ctcgaggcga aagccggtct tcgggtgcgc cagggccagg cgctcgacga
     9901 tctcgcgcgc cagctccagc tcgcgccgct cggacttcag gaacttgagc cgggccgggg
     9961 tattgaagaa gaggtccagg acatcgacgc gggtgccggc cggggccgct gccggctcgg
    10021 ccgcgcccag ccggccgcca tcgccggcga gccgccagcc atgcgcctcg ctggcggtgc
    10081 ggctggtcag ggtgaagcgg ctgaccgagg cgatcgacgg cagggcctcg ccgcgaaagc
    10141 cgagggtccg gatgtcgacg agctgctcgt ccaggagctt cgaggtggca tgccgctcga
    10201 ccgccagctc cagctcggcc cgggtcatgc ccgagccgtc gtcctcgacc gtgatggcgc
    10261 gccggccggc ttcctcgagc gtcacccgga cgcgggtggc gccggcgtcg atggcgttct
    10321 cgaccagctc cttgacggcc gaggccgggc gctcgaccac ctcgcccgcg gcgatgcggt
    10381 tgaccaggcc cggcggcagg cggcggatgc gcggcgccgg cccccgggcg gtgggtgcgc
    10441 tgctctcagc ccttgccgcc attgccgttg gccgcgacca gcgggctcag gcgggtcgca
    10501 ccgtcgaggc cgacgggatc gccgaccacc gagaagagcg gctcggcgct gaacaggcgc
    10561 tcgctcgcgc gggccacgtc ggcgagggcg accgcctcga tgagcgcatt gcggcgctcg
    10621 agatagtcga tgccgagatc ggccacctgc atgccgacga gcatgcgggc gacgctctcg
    10681 ttgctggtga agcggagcgg gaaggagccg gtgaggtagg tccgggcatt ggcgaggtcg
    10741 gtctcgccga tatcgccctc ggccatgcgc ccgagctcgg cccggatcac ctcgatcgac
    10801 tgcgccacct ggtcgttcct ggtcgccacc ccgcccatcc agagcggggc gagctcggcg
    10861 tcgacgaggt gggaatagac cgagtaggcg agcccccgct tctcgcgcac ctcctcgagg
    10921 agccgggacg cgaagccgcc gccgccgatg atgtagttgg cgacataggc ggcgtagtag
    10981 tcggggtcgt cgcgcgggat gccgggcagg ccgaaggtca cgacactctg cgggatggtc
    11041 atgggggtga ccagcgtggc gccggtcgcc ggcgtcgccg gcaggagcgg cggcaggtcg
    11101 gccagggccg ggaggtgggc gaaggcccgg tccagcagcg gcgccagctc cgccgcggtg
    11161 atgtcgccgg cgacgccgat gacgaggttg tcgcgggcga ggcggcttgc gacataggtc
    11221 tcgaggtcgg cccgcgtgac cgcgcggatg gcttcgggcg tgccctgcgc cggccggcca
    11281 taggcgtgat cggggaaggc gtgggcgaac cagacgcggc ccgcgaccgt gttggggtcg
    11341 gtgcgccggc gctgcaggtc gctcgtgatc tgctggcgga tgcgctcgat gggctcggca
    11401 tcgaagcgcg gctcggcgag cgccagtcgc agcagctcga aagcccgctc gcggttcccg
    11461 ttgagcgtca tcaggcggcc ggccagcgcg tcccgctcgg cggtgagctg gagccggatc
    11521 gccagatcct cgagctcggt gcgaaaggcg aggctgtcga gctcgccggc gccctcgtcg
    11581 agcagcccgg cgaccatgcg ggacaggccc tcgctgccgg ccgggtcggc ggcggccccg
    11641 ccccgaaagc tgaaggcgac cgccagcagc ggcacgctcg gcgcgttgac cagccacgcc
    11701 tcgaggccgc ccggggtcag gacccgctcg acgtcggtgc cgaaagcagt cgtggtggcg
    11761 ggcatggtat gcggcaagac ctgctatccc gtggctgcgg cggcggtggc tgcgtctgca
    11821 gtggcggcgt cgccggccgg gcgcttgccg ttgccattcc gggccggcgt gaggcggccg
    11881 gtgaccgagc ggccgcggtc gaagagctcg cgggcggctg cctccaccgc cgggcgggtc
    11941 acggccgcga tgctggccgg ccagctctcg acgtcctcga tcgtcgagcc ggtggtcaag
    12001 gcgctgccga agacgcggac cgcggtggtc aggctgtccc gggcgtagac cgattcggcg
    12061 atcagccgcg ccttgacgcg ctcgacctcg gcctcggtga cgccgtcctc gaccagcctt
    12121 tcgatctcgg cgtcgagggc cttctcgagc cggtcgagat cgacctcgcc gcgcgggctg
    12181 ccgtagatgc gaaaggccgt ctggtcgagg ctggtgcccc gataataggc gccgatgccc
    12241 gaggcgaggg cctggtcgac gacgatggcg cggtagagcc ggctggtgcc gccgccgccg
    12301 aagagctcgg ccaggacctc gagcgcgtgc gcccggccat cggccgcggt ggcgtggctc
    12361 ggcgccaaat aggagcgcac gaggctcggc tgctcgaccc gctcgtgcgc cagctcgatg
    12421 cgccgctcgg cgagctgcgg cggctcgacg aggcgccgcc gctcgggcgt cgggcggggc
    12481 gccacccggc cataggtccg ctccgccaga ggccgcagct cgtccatggt gatgtcgccg
    12541 gcgaccacca ggatggcgtt gttgggggcg taccagaggt catagaaggc gagcgcgtcc
    12601 tcgcgcgtgt agctcgcgat ctcgtggtgc cagccgatga tcggcagccg gtagggatgg
    12661 ttcaggaact gggcggcgtc gagctgctcg tggagcatgg ccccaggctg gttgtcgacg
    12721 cgcgagctgc gctcctcgag gatgacgtcg cgctcggcca ggacatgggc cgcatccagg
    12781 cggagattgg ccatgcggtc ggcttccatc tccatcacca tggccagccg gtccctggcg
    12841 atgttctgga aataggcggt gaagtcgctc gatgtcatgg cgttgtcgtt gccgccgtgg
    12901 cgggccacga tcttcgagaa ctcgcccggg gctatggcct ccgtcccctt gaacatcaga
    12961 tgctcgagga aatgcggcag gccggacttg ccgggcgggc tgtcggccgt gcccaccttg
    13021 taccagaccc agtgcgagac gactggcgcg cgatggttct cgatgacgac cacctgcatg
    13081 ccgttgtcga gcgtgaaggt ctgcggatgg aacatgccca tgggcgcccc tgcctcggcc
    13141 gcgaggttga gacccgtcgc catggctccg gcttccggca tcgccgtcgc ctccctattg
    13201 ctcgggtgcc gggggctcga ccaccgacgt gccgacccgc tgggtgacga cgcgcgcgct
    13261 ctggccctcc gcctcgaggc gcgccgcctc ggcggtgggg tcgagcggct gatagacgcc
    13321 ggagctgtcg accatgcgcc gcctctggaa atcgaggatg aagaggaagc ggctctcgtc
    13381 gagctgggtc atctcggtgt tctcctcgag gatcacttcg cggatgttgg gctcgacggt
    13441 gccgggcagg ccggcgagga gcgcttcctc ggccgggctc gggtcggcct ccgggaccgg
    13501 ctggccgaag agcagctcct gggtctgcag ggacgccgtg ccctgctgct gcgccgggct
    13561 cggcgtgccc ggcttcggca gggtgtagct gggtggcagc accagaggct ggcggcgcac
    13621 cacctggaac tcgtcgggtg cccgccggcc catgccgagc cgctcctgca gcgtgccgct
    13681 gccgcagccg gtcagcgcca cgaggacgag cgccatgcag ccgaaggcga tccgacttcc
    13741 ctggcccgct cgcttcactt ttgtctccct gcgacaggta gcgcttcccg gccgcccagc
    13801 aggctgtcgc ccaccagcag agccgcgccg acgacgatgg cgctgtcggc cacgttgaac
    13861 gccggccaat ggtagccgaa agcatggaaa tcgaggaagt cgaccacggc gccatagcgt
    13921 acccggtcga cgacattgcc gagcgccccg gcgaggatca gccagatggc gaggcgcggc
    13981 agcttccgcc gctcacggcg cagccagacg acgaggaggg ccgagaccag gagcgccacg
    14041 ccgacgagca gccagcggcc agcctcgctg ccggcctgga acatgccgaa gctgacgccc
    14101 cggttccaga cgaggacgag gttgaagaag ccggtgacct cgacgaagcc ccggtcggcg
    14161 aggctggcga gcgcccactg cttggtcagg aagtccagga ccaggatcag gaggaagacg
    14221 ccgggtccga gccagcgccg ggccgggtcg ttcctcaagc cgccatggcg ccgaccgccc
    14281 cggtgcagcg gatgcagagc ccgccggcct cgtcgacctc ctcgagcacc tgccagcagc
    14341 ggccgcattt ctcgcccgcc gccagcgccg gcacgacccc cacgcccggc acgtcggcga
    14401 gcgtgaaggc acccccgggc ggggttccga ccacgatctc gatgccgctc gtgatgcaga
    14461 gcccggccat gtcgagcccc tcgatggcgg cggcatgcgc ctcggtcaca tggacgacag
    14521 gggcggcctg caggctggca ccgatgcgtt tctcgcgacg ctcgatctcg agcgccccgg
    14581 tgaccacgcg gcgcaccgcc ctgaccttct cccagcgggc ggcgagcgcg gggtcgcgcc
    14641 agtcgcccgg cagcgtctcg aagagctcga gatggatgct gtccgtctcg ccctggccgc
    14701 gggcgagcca ggcttcctcg gcggtgaaga cgaggaccgg ggcgagccag cgcacgaggc
    14761 agttgaaaac ccggtcgagc acggtgcggg cggcgcggcg ccgcacgctg tcgaccgcgt
    14821 cgcagtagag gctgtccttg cgcacgtcga aatagaaggc cgagaggtcg atggcgcaga
    14881 aatgatggat ggcgccatag agcctgggaa agtcatagct ggcgttgcag gagcggacga
    14941 ggtcgtcgag ctccgccagc cggtgcagga cccagcgctc gagctccggc atccggtcgc
    15001 agggcaggcg ctcgacctcg cggaagccgg cgaggttgcc gagcgcgtag cgcagcgtgt
    15061 tgcgcaggcg gcgatagttg tcgccgatgc cgtccaggat ctccttgccg atgcggatgt
    15121 cctcggcata gtcggcggag accgtccaca ggcgcaggat gtcggcgcca tgggtcttca
    15181 tgaggtcgat cggcgaaagg acgttgccct tcgacttcga catcttctcg cccttggcgt
    15241 cgacgacgaa gccgtgggtc agcacggcat cgtagggtgc ccggccgcgg gtgccgcagc
    15301 tctcgagcag cgaggagtgg aaccagccgc gatgctggtc ggagccctcg agatagaggt
    15361 cggccggcca tttcagctcc ggccgctgct ccaggacgaa ggcgtgggtc gagcccgatt
    15421 cgaaccagac atcgaggatg tcgtcgacct tctcgaagtc ttccttgcga tgatcgttgc
    15481 cgaggaagtc ctgggggtcc ctggtccacc aggcatcggc gccctcggcc tcgaaggcca
    15541 gcgcgatgcg ctcgatcacg gccgggtcgc gcaagggctc gcccgtcttt ttgtcgacga
    15601 agacggcgat cggcacgccc caggcgcgct gccggctgat gcaccagtcg ggccgcgtct
    15661 cgatcatcga gcgcagccgg ttcttccccc tggccgggac gaaggtcgtg gcgtcgatgg
    15721 cgtcgagcgc cttctggcgc agatcgagct cgccctccat cgggatgaac cactgggccg
    15781 tggcgcggaa gatcaagggc gccttggagc gccaggaatg cgggtaggaa tggacgagcc
    15841 tgccctgcgc caggaggttg ccctcggccc tcagggcctc gaccaccggc tccgccgcct
    15901 tgaagacgtg aatgccctcg aagcccggca cggccgccgt gtagctgccg tcctcggcca
    15961 cggtgtccgg cacctcgagc ccgtggcgcc ggccgagctc gaagtcgtcg gcgccgtgcc
    16021 cgggggcgat gtggacgagg cccgtgccgt cctcgtcgct gacgaagtcg gccgggtgca
    16081 gcggcacctc gaagccgtag ccgccggcgt ggccggcgag cgggtgggcg gcgatcgagc
    16141 cggcgagcgc cgtgcccttg aaggtctcga gcacctgcca gtcggcgatg ccggccgtcg
    16201 ccatggtggc ctcgaccagc gtctcggcca tgatcagccg gcggccggcg gccgcgacca
    16261 gggcgtagtc gatggcggcg ccataggcca cggcccggtt ggccgggatg gtccagggcg
    16321 tggtggtcca gatgaccacg gcggcatcgg cgaagcgtgg ctgcgccggg cggatgaggg
    16381 gaaaggccac ccagatcgtg gtcgaggtgt ggtcgtggta ttccacctcg gcctcggcga
    16441 gggcggtctt ctcgaccacc gaccacatca ccgatcgctt gccgcgatag agcgagccgt
    16501 tgagcaggaa cttcgccagc tcgcgataga tgtcggcctc ggaccggaag tccatggtcg
    16561 cgtagcgccg gtcccagtcg ccgacgacgc cgagccgctc gaactcggcg cgctggatgt
    16621 cgatccagcg ggccgcgtac tcgcggcact cgcggcggaa gtcgatgatc ggcacgtcgt
    16681 ccttgttctt gcccctcttc cggtactcct gctcgatctg ccattcgatc ggcaggccgt
    16741 ggcagtccca gccggggaca taggccgcgt ccttgccgag catctgccgg ctgcggttga
    16801 cgacgtcctt gaggatcttg ttgagcgccg tgcccatgtg caggtggccg ttggcgtagg
    16861 gcgggccgtc atggaggatg aagcgctcgc ggccacgagc ggcctcgcgc agccggccat
    16921 actgcgcctc ggccgcccag cgctcgagct gggccggctc gcgcgccggc aggttcgcct
    16981 tcatggcgaa gtccgtgcgc ggcaggaaga cggtgtccct gtagtccttc gggtagtcct
    17041 tggtcatccg gtcgtcatcc gatcgggcgc gccttcggcc cggcgcgccg cgtcaagcgt
    17101 cgagacggtc atggattcgc cgcgccgcgg agcaatcctc tgcgatctgg gccttcaggg
    17161 cctcgatccc ggggaacttc acctcgccgc gcaggcgttc caggaacgcc acatcgagcc
    17221 gctgtccgta gagatcgagc cgagcatcga gaaggtgcac ctcgagcagc agccgatcgc
    17281 cgtcgaatgt ggggcggcga cccagattcg cgatgccggg ataccagacc ttgcctgccg
    17341 gtgtacagcg gccggcgcgc accgcgtaga cgccgctggc cggcagggca tagcgttcgg
    17401 cggcgggggc gatgttggcc gtgggaaagc cgatggtgcg accgcgccga tggccttggc
    17461 cgacgatgcc ggtgagctcg aagggatagc ccagcaggcg gttggcgagc gccagctcgc
    17521 cggcggccag ggcgacgcgg atccgggtcg aggagcaggg cagcccgtct gccatgaccg
    17581 gcggcacggc ggcgaaggcg aagtcgtggg cggcggccgc ctcctgcagg agcgccgcgt
    17641 cgccctgccg ccggtggccg aagcggaagg cctcgccggc cgcgaggccc ctgatgccga
    17701 gccggccgac gaggatacgc tcgacgaatt ccggggcggg catcgccatg agagcccggt
    17761 cgaagcgcaa gaggaagagg tgatcgacgc cgaggtcgcg caggagcgct atcttccgcc
    17821 gcagcggcgc gaggcgcaga ggtgccgtcg ccgggctcag cacctcgcgg ggatgcggct
    17881 cgaaggtgac gacgccgagg ccgccggccc gcgccacctg gccgtgggcc gcctggccgt
    17941 gggccgcctg gcggcgcgcc gcctcgatga cggcgcgatg cccctggtgc acgccgtcga
    18001 aattgccgat ggcgaccgca cagccgaccg cctcgggcgg caaagctgcg aaaccccgat
    18061 ggaccctcat gcgccgcctt cttccacggg ccgcgaagcc tgtaaagctc ggcccgtggt
    18121 cggtttccgg cgcggcccgg cggtccgggt cctgccagcg gaggctgggg ggcgtctagc
    18181 ggcagaggct ggcgatcgcc cgctccaggc cgggccggtc ggttacccgt tgccggtagc
    18241 cgacctcgcc ggtgccgacg acggtcggcg gcgttgcctt gctgccgtca ttgatccggc
    18301 cgacgacgaa cgggtcgccc gacgggttgc gccaggtgag cgtgctgccg gtgacctgcg
    18361 gcgcgtaggc gccgccaccc gggtggatgt ggtcggtgac gggccggccc ttgaccggaa
    18421 cgacccacac cccccggccg ctggtgttga agcggtaggt gatgtagtcg ccggcggcga
    18481 cctcctcgga gctgcgggcg aggaagaagg cttttggctt ggattcgctg ccgatcgcgg
    18541 agttggtcac ggcacgggcc acatagtccc agtactgcgc ggtgccgcgg ccgtccggac
    18601 cgcggtaggg cttcttgtcg ctggcgtagg acggcagcac gtactcgccc tggtagcccc
    18661 aggtcgcgtc gatcctggcg gcgtaggagt ggcgcacgcc gccggtctcg aacttggtga
    18721 agtcgctgtg cgagacgtac cggctgaacg agccgccctt gtagtcgcgc cagccgaaga
    18781 gcggctcggc gatgatccgg cagcgccaga catccggcca ggcctgccat ttgccggccg
    18841 ccgtgtcgcc accgagctgg gcgaaatcgc cgaagtcgat gctcgacgtc ttcaggttca
    18901 tgcggcccag gacgatgaac ggccgcaccg cgtccttgtg ggtgcggatg cggaccagcg
    18961 tgccgacacc ttccgtgcgc ttgccgttgg gcgaggtcag ccagccggcc ggttgcggat
    19021 cgaacgaggc gccgatccag tagacgccgc gaaacccctc gacgacgatc tggaacttgc
    19081 cgttgatcga ctgattgggg ctgaccagca gaagatcctg gtcggcccgg ccggtcacct
    19141 tctggaagcc ggaaggctgg acatagtgga tgaccagatt ggcggcgggc gaggggatgg
    19201 ccgggacgtc ggtcgggctg ctcgatccgg agccgccgga ggccgggctg ccggccgtgg
    19261 agccgccgga agtggcaccg ccggcggtcg ggtttccggc agtggcgcct ccggcggtgg
    19321 agccgccgga agtggagccg ccggaagtgg agccgccgga agtggtgccg ccggaagtgg
    19381 cgatgccgga acccggcaag gtgagcgccg ggaagcccgg cagcagccgt tgctggccga
    19441 gcttcagctt tgggatcgca tccccggcgg ttgcctggcg gcccgggagg ccgagctgga
    19501 acgagttgcg gaagctgaag ggctcggccg ccagggcaag aggtgccagc gtcggctgca
    19561 gcacggcggc ggccgcgacg atcgtgccga gcgccaggat gcgaaggaat ggcggcacga
    19621 cgtgtcgagg cacccggcac cccgtcgcaa cgtcgcgggc ggcctggttc cggctggtgg
    19681 cagccggcgt ggctcccttg gtgggtccgc ggctgcggtc cggggtcatc gcatcttctc
    19741 cttgattgtc cgcgctggct ggcatgcctg ccgatcccgg tcgcccgctg ggtcgctcgc
    19801 gggcaggtgg acgagctccg atggcggcat tcgacccgtt cgttgtaacc cgggtagcaa
    19861 aaccgaagag cagtatctag aactcaatcg agaaaacttt tggttaagaa aaattccgta
    19921 ttcgtgatca aatcagtatc atgatactgt ccatgcggca tggtttcggc tcgcggttgt
    19981 ttatcaacca gagcgtgcat aatcgtcgaa ttctcgcttg tttggtgttt cgtggacgcc
    20041 gaggttgtga cgcggccatc acccttgtaa gagcgggtga tgcgccgcgg gcacggcggt
    20101 tctcacaatc atccgggaag gggcgtccga tgagacctcc cgctttgtcg cgacggttcg
    20161 cggccatagg cattttcttg tcggtactgg cgctcggcgc ctgcgagacg ccggtcgagg
    20221 gatacggggc agtcggcaac agcttgagga ctgtcgactt cgatccaccg ccgggcggcg
    20281 agaccgcggc ccgctcgccg accccgacga ccggtggcac ggcgacgggc gaaaggcccc
    20341 agtcgcagct cttcttcggc cagaacccgg tgccgggagc ggagaccgct gccgccgctg
    20401 acacgaccgg cggagtcagg ctgaacttcg atggcgccag cctgcgcgag gtcgtggacg
    20461 tggtgctcgg cgagatcctg ggcgtgagct acacgatcga tccggcggtg agcggcgacg
    20521 tgatcctgtc gtcgaccgca ccgctctccc gcgccgacat gctgaccgtg ctcgagacca
    20581 tcctgcagat gcacggcgcc acgctggtcg atctcggcgg cacctacgcg gtgatggcgg
    20641 gagcacccac ggtcggtgcc gcgcagttca cgaccgtcgg cggcgagcag gcgccacccc
    20701 tgatcgtcgg caccggcgtc accgtcgtgc cgctgcgcta cgtgagcgcc gccgcggcat
    20761 cgcagttcat ccagcccctg ctctcgctgc ccgagcagat ccgcatcgac gacgcccgca
    20821 acctcctgct cttcgtcggc aatgcggcgg aacggcagaa cgtggtccgc acgctcggcg
    20881 agatcgacgt ggactggatg gccggccggt cggtcgggat cttccccctg agcacggcga
    20941 cgccggaatc cgtcatcgcc gagctcgatg gcctgttctc gcccttcggc ggcttcgata
    21001 tcaacacctc gggcgtgcgc ttcctgccga tggaccggct gaacgcggtg ctcgccatca
    21061 ccagccagcc cgagcagctc gtcgacatcg agcgctgggt gcagcgtctc gaccggggca
    21121 gcgggtcgag cgtgcagttc ttcgtctacc agctccagaa cacgccggcc ttgccgatgg
    21181 cggagcttct gaacgccacc ttcgccgacg cggcggccgc cgatctggtg ccggcctcgg
    21241 ccgggctcgc cggcgagttc ggggcggtgc cgtcgcaggc cgggctcgag accggcgact
    21301 ttctcggcga cacggcgccc ctgcccttcg acgcgccatt cggcgacctg ccgcccagtc
    21361 agcgtcgcga cgtgctgtcc tcggtcaagg tcgtgcccaa cgagctctcc aactcgctcg
    21421 tggtgcgcgc cacgccgcag gcctatgcgc tgatcgagca gaccatccgc cggctcgacg
    21481 tggcacccct gcaggtgctg atcgaggcga ccatcgccga ggtcgtgctc aacgaccagc
    21541 tgcgctacgg cgtgcagtac ttcttccagg ccggcagctt cagggccggc ttcaacacga
    21601 cgaccgacgg tggcgccagt ggcgcagtca atcccaatct gctcgacccg ctggctctcg
    21661 tgccgggctt caacttcgtc tattccggcg ccagctcgaa catcaccatc gacgcgctgt
    21721 cccgcatcac cgacgtgcag gtcctctcct cgccctcgct ggtggtccag gacaacagcg
    21781 aggcggtgct gaccgtcggc gacgaggtgc cgctcgtcac ccgcacctcg cagggcgtgg
    21841 aaaacccgga ctcgcccatc gtcgccaaca tcgagtatcg cgagaccggc gtcatcctcg
    21901 aggtgaagcc gcgcatcaac ttcaacaaca tcgtctcgct gcagatctcg caggaggtca
    21961 gccgggtgca gccggtcggc gaggaccagt tcggcaaccc gaccatcgcc cagcggcgga
    22021 tcaccagcag cgtcaacgtc accagcggcc agaccgtggt cctgggcggg ctgatccagg
    22081 acgacacgat ccgcaacaac agcagcgtgc cggggctcgg cgacatcccg gtgctgggca
    22141 atctcttcgg cagcaccaac gatgtgtcgc agcgcaccga gctcatcgtc ttcatcacgc
    22201 cccgggtgat ccgcaacgcc gacgacgcgc gggccgtcag cgaggagctg cgcgaccgga
    22261 tgcgggccgc ccagccgccc gccgactccg cgggcgagcc gctgacgccg gccggcgcac
    22321 ccccgcccgt gccgacgacg accccgccgg cggtgcccga gccggccccg ccgccggcgc
    22381 cgcccgcgac gacgatcacg ccgcgacccc tgacgaccag cgagctgccc gagccggcgc
    22441 tgcctgaccc cgagccggcc ggagcaccgg cggaggcgcc cggcgaggag ccagtacccg
    22501 gcatcgtgcc ggtcgcctgg gtcgagccga cggccgtcgt gccgccggtc gccctgccgg
    22561 tcgtcgagcc gccctccgtc gggccgccgg atggcgagcc gccgcgccgc cggccgtcgg
    22621 tcgacccggc cgcggcggat gccccgccgg ccaacctgcc gctggcccgg ccgattccgg
    22681 gggatgtggt cctgccggcg ccgcgccccg gcgaggtggg catcgccctc taggcgaggc
    22741 cgcgctgggc gccatcgctt tcgcctgggc cctggccgcg gccctcgtcg gcctcgcggt
    22801 cggcagcttc ttgggcgccc tggcgtcgcg ctggcccgcg ctcgaccggg gcttcctttt
    22861 cggccggtcg cgctgcgccg cctgcggggc ggggctcggc tggcgggaga ccctgccgct
    22921 ctggagctgg gtcgcgcaac gcgggcgctg ccggcattgc ggcggcgcca tcggctggcg
    22981 gccgctggcg gccgagctcg cgggtgccgc cattgccgga acggctttcg cggcgctggc
    23041 cccggcagcg gccctgctgc tcacgggcgt cggctggtgc ctgctgctgc tggcgctgat
    23101 cgacgctgcg catggccgtc tgccggacat cctgacgctg ccgctcgctc tggcgggacc
    23161 gctggtcgcc gccctggcac cgctgccggg gctggtgccg ccgctcgagg ggctcctggg
    23221 cgccgggctg ggcttcgccc tcttctgggc catcggccgg ctctacgcgg cctggcgcgg
    23281 gcaggagggg ctcgggctcg gcgacgccaa gctgctcgcc gctgccggcg cctggctcgg
    23341 gccgccgggc ctggcgccca ccatcctcgt ggcggcgatc acgggcctcg tggccgcctg
    23401 gctcggcggg cgccggcggg gtggcgacgc catcgccttc ggcccgttcc tggcgctggc
    23461 ctgctggctg ctgctgctcc ggcagctcgc cggatcccag ggggggagct aggcgcccgc
    23521 cttcttgtgg atctccttca gcagcgggcg gatggcgccg aggtcgtagc cggcggtggc
    23581 ggcggcggcg atcagcgcgt ccgggtcctc gtcccgcgcc tcgaccagcg cccagagatg
    23641 gcaggagcgg gtgccggagc ggcagtaggc gacgaagggc ccctggtgcc cgtcgatcgc
    23701 cgcccgcatc gccgccagat ggtcgggcaa gatgccgccc gaaacgaccg gcacgtggac
    23761 ataggcgagg ccctgggccg ccgccagcgc ccgcgcctcg gcatcggcga gctggtcggg
    23821 cgcctcgccg tccgggcggt tgttgatcac cgtcctgatg ccgagctcgg ccgccgcggc
    23881 gaaatccgcc ggctcgagct gcggggcgac gtagaaatcg gatgccactt tgcggaaggt
    23941 cgtcatgggc ggcaggcggt catgcgggct gctcctcgat gctgggatga tcatccgacc
    24001 aaattccggg tcggctgaca agcgccgacg gcatcgcccg gccgcccggc ttgccgggct
    24061 cgcccgcggc ggcatggcgc ccgtggcgtc ggcggcgccg tgcgcatcgg gcccggcgac
    24121 gcagcaggcc gtcacctcgc cgtcggtgcc cggggggcgt ttttcgtccg gccgttcaga
    24181 ccttcttaaa ggttcaccgg tagtctctcc tctcgtgggg cgcggcccat tgatcccctg
    24241 gatcgacggc tcgcggctca ctgtcctccc tgacgttgtg atgcctccct acagactggg
    24301 tcaccgcttc ggcggtggcc cgtttttttc tgcgggggcc atgacggcgg caccggcagg
    24361 caggctctcg ctgccaatac tagggatatt atcctaatcc ggcccgcccg tcaagccggc
    24421 gcccggttca ttcggcattt cctggccttc cgcacccgga gcacgcgcgg aagctggcga
    24481 gagccctgaa aatacgtttt atttcaattg ttcaacggcg atcgggttcg ttggtcgacg
    24541 cctcggttcg ttcgtcgtag aggcggattc attcgtcgga aaggcgggtt cgttcgttga
    24601 aatcccgggt tcgatcgtcg ccgcctgtcg cggaatccgc gacagtttcg ggcgattgtc
    24661 gcagaatctg cggcataagg aatcgtgatg tcgcagaatc cgcggcagtc ggacggtcgc
    24721 tgtcgcgaaa tacgcgacaa ccctggccct gctgtcgcgg attctgcgac agtccgggcg
    24781 gtgcaggggg tggaagggcg cggagcgacg cttctggggg gctttccgcg gactaggtat
    24841 aagcgttcgt gatggcggcg aggcggtcga ggtcgccgcc gccgggagcg ttgcgtcgcg
    24901 gctgttcggc cacgggttcc tcggcgcggg ccagcagccg gcggcggctg ccgaaaacac
    24961 cttcgagcag ggccggcaga tggagatcgg cgtccagcct ggggaaatgc aggccgagcc
    25021 cggatcggct gatctggatc ggctcgaggt cggccggccg cgcccgctcg agcccctcgg
    25081 tgtcacgggc cgcgaaggcg atcggccccg ccgccatgag tccggcggcg cgcgcctcgg
    25141 ccgcctcgaa cgcctcacga cgcaccacgg atcgtctccc aggcgcggca gggcgccgcc
    25201 agtgccgcgt tcgagattac gaggcgctct tgtcggctca agggtcttgt ggtggctaaa
    25261 atcgtcctcg tatcttctca actgggcgga atacggtgac tgtctggacg agatggccgt
    25321 ggctcggcag ctgccgggcc aaggccggcg gacgggacgc cggatagtcc ctggtcgcgt
    25381 gagacgtggc cgatcaggca gccacccagc cgctcctcga ccagcgcc
//
LOCUS       DAOTFI010000028        25218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2286_length_25218_cov_7.659818, whole genome shotgun sequence.
ACCESSION   DAOTFI010000028 DAOTFI010000000
VERSION     DAOTFI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 25218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25218
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_25218_cov_7.659818"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1628)
                     /locus_tag="P5558_04330"
     CDS             complement(138..1628)
                     /locus_tag="P5558_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRY23570.1"
                     /translation="MRRSRSRLDLRAIVDLRPVAMVVGIVLLVLALAMLPSMLADLIA
                     GHADWQVFLLSASCTAFIGVSLILMTTGVSRTPELTVRQGFLLTTLTWVTTTAFAALP
                     FAFSDLGLGAAGAFFEAMSGITTTGSTVIVGLEAAPPGILLWRAMLQWLGGIGIIVMG
                     IAILPMLSIGGMQLFRTESTDSSEKILPRAAQIATNIALIYLLLSLLCASIYWLFGMS
                     WFDAAAHAMTTIATGGYSTYDASIGHFASPAIEWTAITFMIVGGIPFVLYVQILRGQK
                     LQLFHDVQVRWFLGILAGGTALLAFWLVQRGLMFGHDGLRHAAFATVSVVTGTGYAAS
                     DFNAWGTFPVGVIFFLMCIGGCSGSTTGGMKVFRFAVLYQIARVQVLRLLQPSGVFRP
                     IYNGQPVADQTAISILGFVFVFALSFAVLSILLSFLGLDYLTAMSGALSALANVGPGL
                     GDLIGPAKNFAALPDTAKWLLSAGMLLGRLELFTVLVLFAPAFWRA"
     gene            complement(1652..2044)
                     /gene="apaG"
                     /locus_tag="P5558_04335"
     CDS             complement(1652..2044)
                     /gene="apaG"
                     /locus_tag="P5558_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRY23571.1"
                     /translation="MYSEVTRAISVSVDPFFVEEQSEPADSRWVFGYRVVIENTGSEP
                     VQLMSRYWRIVDGRGRLHEVRGDGVVGEQPMLEPGERFEYTSGTPLGTPTGIMAGTYQ
                     MLGADGLWFDVEIPAFSLDAPGSRGSVN"
     gene            complement(2119..2874)
                     /locus_tag="P5558_04340"
     CDS             complement(2119..2874)
                     /locus_tag="P5558_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY23572.1"
                     /translation="MHRARRDPAMVVLEGLHAVKHALRFGVVPRLVISDAPASVLALA
                     AELAPDVQARLAALLEPVTTAEFAALAPEPPATRLIAVADRPVWPGSSTAAGPIVVLD
                     RPRHPGNLGAVVRVAAAAGAAAVWTLGGVDPWSAAAIRGSAGLHMALPVEAPDELPPT
                     GRPIIALADGGEPLGPGTLPGDAVLLFGSERDGLAPHLRRVASRIVTIPMRPGVSSLN
                     LATAVAVVLYLQRLAGSGASGPADTGQPDPLST"
     gene            complement(2898..3713)
                     /gene="phnE"
                     /locus_tag="P5558_04345"
     CDS             complement(2898..3713)
                     /gene="phnE"
                     /locus_tag="P5558_04345"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRY23573.1"
                     /translation="MPVRELDGRPVWQRRTTRRQWAIWAGWLAGVMLFVWCWKLMTGR
                     TIWAFVWDAPRQAADIGSRMLPPRWSYIDKLLWPLWDTLNIATLGTLLGIIIAVPVAF
                     LAARNTTPSALFLRPVALFIIVASRSINSLIWALLLVSMIGPGLLAGIIAIGLRSIGF
                     VGKLLYEAIEEIDESQVEAVRATGASGAQVLDYGIVPQILPAFAGISVFRWDINIRES
                     TILGLVGAGGIGLQLQASLNTLAWPQVTMILLLVLGTVLVSEWVSAKVRHAVI"
     gene            complement(3713..4519)
                     /gene="phnE"
                     /locus_tag="P5558_04350"
     CDS             complement(3713..4519)
                     /gene="phnE"
                     /locus_tag="P5558_04350"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRY23574.1"
                     /translation="MSTAAAGRRWRRPPVLIQSRRTRWLVYGGLLIYLALAISTVEVN
                     WLRVYEGLDRGWRFAQGFLAPDFVSRWKDISQGLAESLTMTVTSTVVGVLLAVPIGIG
                     AARNVAPMPIYLFCRAIIALSRTFQEVIVAIFFVAMFGFGPFAGFLTLAFATIGFMAK
                     LLAEDIEDIDEAQAEAVRATGASFPQLINYGIQPQVMPRLIGLSLYRLDINFRESAVI
                     GIVGAGGIGATLNTAIDRYEYDSAGAILLLIILIVMAAEYSSSHVRGWVQ"
     gene            complement(4516..5385)
                     /gene="phnC"
                     /locus_tag="P5558_04355"
     CDS             complement(4516..5385)
                     /gene="phnC"
                     /locus_tag="P5558_04355"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HRY23575.1"
                     /translation="MLRLDNLAKRYKTGDHALRGVSLEVDRGQIVGLIGPSGAGKSTL
                     IRCINRLVEPTGGKVYLNDVELTSMSGADLRRVRRRIGMIFQEYALVERLTVMENVLS
                     GRLGYVPFWRSFLRRYPQADVDKAFALLERVGLAEHADKRADELSGGQRQRVGIARAL
                     EQDPELLLIDEPTASLDPKTSRQIMRLIREICEERSLPAVINIHDVLLARMFVDRIIG
                     LTAGEVVFDGPPAELDDAVLTRIYGAEDWTAMQTAAAEDAEDAEDAAAAHGGHVRRAA
                     EGERPEERLAGLA"
     gene            complement(5449..6414)
                     /gene="phnD"
                     /locus_tag="P5558_04360"
     CDS             complement(5449..6414)
                     /gene="phnD"
                     /locus_tag="P5558_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY23576.1"
                     /translation="MTYHPLVAGLATLLLAAAPLTAAAEFQLDSRYSDADGDLIADIP
                     TDPADWIDPPVLVFAYTPVEDPAVYEGVWADFLDHLSEETGKPVQFFPVQSNAAQIEA
                     MRAGRLHVAGFNTGSNPLAVACAGFRPFAMMAAEDGTYGYRMAIITHVDSGIEKVEDI
                     KGRTMAFTAETSNSGYKAPSALLSSKFGMEAGRDFEAAFSGKHDNSVLGVYNKDYDAA
                     AIADQVMIRMAARGVIDQDQIRVIYESDPFPTTGYGVVYNLSPELQEKIKAAFFNFDW
                     EGTSLLAEYGNSDPAQEAFVPITFQNEWSVIREIDAAMDVSYACQ"
     gene            complement(6454..7410)
                     /gene="phnD"
                     /locus_tag="P5558_04365"
     CDS             complement(6454..7410)
                     /gene="phnD"
                     /locus_tag="P5558_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY23577.1"
                     /translation="MLQRTVIASAMLLALGLGSAARAEFTLDPRYTDTDGDLIADIPT
                     DASQLVDPDTLIFAYTPVEDPAVYAEVWAGFLAHMEEVTGKPVQFFPVQSNAAQLEAM
                     RAGRLHVAGFNTGSNPIAVACAGFRPFTMMAAADGSFGYEMEIITYPGSGIEKIEDLK
                     GRTLAFTAETSNSGFKAPSALLKSEFGLEAGTDYEAAFSGKHDNSVLGVANKDYDAAA
                     IANSVKARMIDRGVVSDDQLVVLYTSQTFPTTGYGIVYNLTPELQAKIQEAFFSFDWA
                     GSALEAEFSKSGEAQFIPITFKDDWAVIRQIDEANGVSYACQ"
     gene            7558..8589
                     /locus_tag="P5558_04370"
     CDS             7558..8589
                     /locus_tag="P5558_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23578.1"
                     /translation="MSYRQHCKTLLAGAALATLGWGVAEADELTLYCSPQEEWCQLMV
                     TTFTAETGIDVAMTRKSSGETYAQIAAEASNPKGDVWWGGTGDPHLQAAEEGLTEAYD
                     TPMLPELHDWAVNQYEAADGKTVGIYAGALGFGYNTELLAEKGLEPPACWADLIKPEY
                     AGEVQMANPNSSGTAYTALATFVQMMGEDEAFEFLKKLHANINQYTKSGSAPIKAAGL
                     GETMVGIVFQHDAVTQKVAGFPIEVVSPCEGTGYEIGSMSIIKGGPNPESARKFYDFA
                     LRADVQTLARDAQAYQVPSNRNAEAPPESPDFSTIKLIDYDFVTYGSSDTRTRLLARW
                     DAEVGSLPN"
     gene            8601..10808
                     /locus_tag="P5558_04375"
     CDS             8601..10808
                     /locus_tag="P5558_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY23579.1"
                     /translation="MSQQRSPLGYWLAIGWAGFLLLPWYVQSGGFFDFAWLLAWADAD
                     AAPALAQAVLHGRWWLLPLALPLALPILLLGRRQDTPAAARLLILAGGIGLAWLALQG
                     FAVIHSGWGFDILARTFGPLELSQSGMGAGAALTALALLMFTGLGIAARGAVGGDRFV
                     VGAITLIVALVAVFIFFPVLRILAGALQDSDGSFAPGLFAARFIDPRIWGLGCVLEGS
                     RCGVAWNTLALALFTGLTTTLLGLAFALVATRTAFPAKRALRALTILPIITPPFVISL
                     AIILLFGRSGTVTQFVADLFGVSGGRWIYGFWGVWLAQILSFTPIAFLVLIGVVEGIS
                     PSLEEAAQTLRASRWQTFTTVSLPLMRPGLANAFLLGFIESMADFGNPLVLGGNFQVL
                     STEIFFAIVGAQFDPGRAAMLAMVLLSFTLLAFWAQQRWLGRKLYTTVGGKGDSGLAA
                     SLPTGLKWLVLGVTVPWAIFTAIMYAMILFGGFVELWGRNHSFTLRHYEATFGITQGQ
                     FGLVWSGTAWNSFWTTLQIAVIAAIPTALIGLITAWLLSRQTFRGKGAFEFGTMLSFA
                     IPGTVIGVAYIMAFNVPPIELTGTGMILIIAFVFRNMPVGVRAGVATLAQLDKSLDEA
                     SLTHRASTLQTVRRILLPLMKPALLAALVYSFVSAMTAISAVVFLVSARYELATTYIL
                     NRVENGDYGIAIAYSSVLIVVMLIVIGLFQLAIGERRLGRRSAAPAGFTGGVR"
     gene            10805..11863
                     /locus_tag="P5558_04380"
     CDS             10805..11863
                     /locus_tag="P5558_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23580.1"
                     /translation="MSSAATVGFENVTKAFGAVVAVRDVSFEVAPGHLVTLLGPSGCG
                     KTTTLRMIAGLELPTAGRIRIGERDVTNLPATDRDVAMVFQSYALFPHMTVLENVGYG
                     LSVSGRPKAEVKERAETTLELVGLEGFGARLPSELSGGQQQRVAVARAVVLEPEVLLF
                     DEPLSNLDAKLRRRVREEIRELQQRLGLTAVYVTHDQEEALAISDRIIVMNNAVIAQE
                     GVPRDLYEAPADRFVADFIGDANVLAVTIGAIDGEHAEVRIGEARRRLLHRGLAPGPA
                     HLAIRPEGIVLTADPEASIMAGRVRRASYLGSHVEYDIETALGELFVTDSDVDRIHPV
                     GSEVGLGFGTHGVTLVRD"
     gene            complement(11874..13208)
                     /locus_tag="P5558_04385"
     CDS             complement(11874..13208)
                     /locus_tag="P5558_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRY23581.1"
                     /translation="MQKSKEFPEARLIASCRRCNAVALPFIGSTLRKGYATFLSQASR
                     QGKEPFATMSASPDPRRLGQWQTTTTAGEPLSAFIPPPLPPVPPLAMEGLYRAFETAS
                     QALGRLDGVSTILPDASLFLYTYVRKEALLSSQIEGTQSSLSDLLLFELDETPGAPLD
                     DVVEVSSYVAAMNRGIDRLAEGMPISTRLIREMHGILLAKGRGATQQPGELRRSQNWI
                     GGTRPGTAHFVPPPFERVPDLMSDLEKFIHAEKPALPVVIRAGLAHVQFETIHPFLDG
                     NGRLGRLLITLMLIAEGILKEPILYLSLYFKTHRKEYYELLQRVRERGDWEAWLDFFL
                     AGVAETSKQAAETARQILNLFETDRQRIEQLGRPAASALRVHQQLQQKPIVVIQDAAA
                     RLGISTPTIGKSIRHLEELGIVREVTGRQRRRVFVYPAYLAILSLGTEPLAR"
     gene            13407..14630
                     /locus_tag="P5558_04390"
     CDS             13407..14630
                     /locus_tag="P5558_04390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY23582.1"
                     /translation="MTQFADFGLAESLLRALQSEGYTSPTPIQTKAVMPVMEGRDLLG
                     IAQTGTGKTAAFALPILHRLFITDKKPLQPRAVRTLVLAPTRELAAQIAASFETYGRH
                     LRPAVTVVVGGVKHNPQVRALARGVDVLVATPGRLLDHLQGGTVRLDQTEIVVLDEAD
                     HMLDLGFLVPIRRILAKLPKRRQTLFFSATMPKDIGELARDMLSEPIEVSVAPVATTV
                     ERVDQSVYLIDAADKRTLLAELLAKPEFSRALVFTRTKHGADKVARHLEAAGIPAAAI
                     HGNKSQSQRERALAAFKSGRSRILVATDIAARGIDVDAVSHVVNFELPDVPEAYVHRI
                     GRTARAGAEGVAISFCDAAERRNLRDIERLTRQTLPVIDRRRPGSANRTPAPAERGRP
                     QRPQQQRRSPSRRAA"
     gene            14773..15612
                     /locus_tag="P5558_04395"
     CDS             14773..15612
                     /locus_tag="P5558_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY23583.1"
                     /translation="MSPLVFAVVLLAAVLHASWNALVKSGGDPYLRLAIVNLTHTVAV
                     LPLLPFVPLPDAAAWPWLLGSVFTHLAYYLFLAAGYRVGDLSQIYPIARGVAPPLVAL
                     GALLVAGEVLSPTGSFAIALVCLGIWWVAGGGPTNRRPLLLALGTGGTIAAYTVCDGM
                     GGRASGDVLAYIVWLFFLDGWPFALLVGLRRGRHLARTLPQAWKPAVGGGLMSICAYG
                     LVIWAMSIAPMAYVSALRETSVLLAAGIGAFLLGEPFGRRRMLAAGVVVIGVVLLQIS
                     RQG"
     gene            complement(15631..15975)
                     /locus_tag="P5558_04400"
     CDS             complement(15631..15975)
                     /locus_tag="P5558_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23584.1"
                     /translation="MSSRANPSKLNALQLKTLAILQEVARSGGDVSEELADGSVALNR
                     LPHAHGDHFHVGSAVVRAKDATGLGNPGVFGALRRKGLIAMAPDGRPALTAAAMSYET
                     GVAEAILHRSDH"
     gene            complement(16072..16839)
                     /locus_tag="P5558_04405"
     CDS             complement(16072..16839)
                     /locus_tag="P5558_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HRY23585.1"
                     /translation="MSERPVAIVTGARRGIGRAIALGLAGRGFDLVLNDLEPGAELEA
                     TAEAVRAAGAQAVAALADIGDLESHAGLLDAAFAFGPVACLVDNAGVSVLQRGDLLEL
                     TPESWDRCQRVNTRGTFFLTQAFARRLIEQAPSPVHRSIVIVSSSNATAASLNRGEYC
                     VSKAGLAMVTKLFALRLAPLGIGVYEIRPGIIRTEMTAPSRERYDAFFAADGTPMPRW
                     GEPEEVAKAVVACAAGELPYTAGQPIAVDGGLTLSRF"
     gene            complement(16886..17617)
                     /locus_tag="P5558_04410"
     CDS             complement(16886..17617)
                     /locus_tag="P5558_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY23586.1"
                     /translation="MLLLGGALIAGFVSGFAGFGTGLVASGFWFVALPATSVPPMIVF
                     TSVIAQLMALHRLRQSFDWRGVSPYLAGGVLGLPFGIMALQVASASLLRVTVGIFLVA
                     YALVQLSAIRNWSVGTWGGWRADMAIGGGGGFLGGFAGLSGPLPLIWLQLRGGPSTRQ
                     RMTYQPFNLIILALAGLGMLLAGQIDGSVVRAVAWCLPVTVLGTWIGLRLYSAAGEAL
                     FKRIVLILLLISGGILVTQSITALH"
     gene            17813..18160
                     /locus_tag="P5558_04415"
     CDS             17813..18160
                     /locus_tag="P5558_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRY23587.1"
                     /translation="MSSNTGTDETLKARTRLEAELAELRATSVATASDRRPVELDQAS
                     VGRLSRMDAMQIQAMAHAADRQRQQRIRRVEAALERLADGDYGWCVRCGEAIASGRLE
                     ADPAVPTCLACAV"
     gene            complement(18104..18595)
                     /locus_tag="P5558_04420"
     CDS             complement(18104..18595)
                     /locus_tag="P5558_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23588.1"
                     /translation="MPLVLGFALTLLVWVALGDAHLALAPGLARVIGPSPLPGLNAIQ
                     LAKLAILAALALMVLGAILVALHAPHDRHERRLAQRFVVTLVVGGALSVGLDLAGMLG
                     QVRLPAVEALVEAATFAACLLLLARHAMRSLVPLRRTVAGQSRSDGAGEAGGHRGIGL
                     EPT"
     gene            18891..19592
                     /locus_tag="P5558_04425"
     CDS             18891..19592
                     /locus_tag="P5558_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HRY23589.1"
                     /translation="MSDEAELNLRELDDEELVKQIQDDLYDGLRDEVVEGVDILLERG
                     WEPYRVLTEALVGGMAIVGVDFRDGILFVPEVLLAANSMKGGMAVLRPLLAETGAPTV
                     GKLVIGTVKGDIHDIGKNLVSMMMEGAGFEVIDLGINCDADKYLKALDEHKPDFLGMS
                     ALLTTTMPYMKVVIDTLIEQGIRDDYVVLVGGAPLNEQFAKAVGADAYCRDASVAVDT
                     AQAFMARKHNQRASA"
     gene            19596..20153
                     /locus_tag="P5558_04430"
                     /pseudo
     CDS             19596..20153
                     /locus_tag="P5558_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
     gene            20200..20505
                     /locus_tag="P5558_04435"
     CDS             20200..20505
                     /locus_tag="P5558_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HRY23590.1"
                     /translation="MAQLTILYWRDIPAQVIARAGRRTAKRQLPARFEQAIDRAAMQA
                     KLTGTEAYLEQWRKSDPTDCGDDLEAAAEEAAAALEAAFTPEQLRDYVMNGGHTPRA"
     gene            20589..21899
                     /locus_tag="P5558_04440"
     CDS             20589..21899
                     /locus_tag="P5558_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23591.1"
                     /translation="MNELSNWSRTTWIVVGALLVIAVLGWGFALDRDRRLEEHASQIT
                     VLEGEQSALGQRFAELETTLADERQAAGDLAALNEQMATAQAELETVEAAKTEAEAAV
                     EAAQGTVAEAEQRVNDLTDEAKEAEERLAGLAEEIGTAEATLAERSEEVTRSETALAR
                     VGELEARVTELSAQVAAAESRLAALNEEAGSVESTLVARQSELDEVTTRLAEAKSLAE
                     QQAGEARGLEARITELTGAVAGEESRLAALQAEREQTEASLETLAAGLESAEAELATA
                     QAKHEATVAETTGLEQRRADLVNEIGTAEARLQEIQGQAGTAEAELAARQGAVEEAEA
                     RLAETQAATAEQQAQLEGLEAEVRQLQEQAAAARRELQQAQTAEQALREQITALEDQR
                     TGIETLVGETQSQLEAQQGELEALRRQLTTEQPQAAPTQAAPAQ"
     gene            22015..22905
                     /locus_tag="P5558_04445"
     CDS             22015..22905
                     /locus_tag="P5558_04445"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HRY23592.1"
                     /translation="MTETVVASATKTVRIGFERPFCVIGERINPTGRRKLAAEMAAGD
                     FSTVEADTLAQVAAGASMLDVNAGIPLADEPAILARTIELVQSLTDLPLSIDSSIVAA
                     LEAGLAVYKGRPLVNSVTGEEERLEAVLPLIRKYDCAVVAISNDETGISEDPDVRFAV
                     AKRIVERAQDHGIEPCDIVVDPLVMPIGALGQAGNSAFRLIRRLREELHVNTTCGASN
                     VSFGLPNRHGLNAAFLCMAAGAGMTSAILNPLHAEEMTAVMGADVLLGKDRDCRSWLT
                     RFREPTAEGTSRNDRRRRRA"
     gene            22925..24931
                     /locus_tag="P5558_04450"
     CDS             22925..24931
                     /locus_tag="P5558_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRY23593.1"
                     /translation="MTDPLVVFTPSGKRGRFPVGTPVLTAARQLGVDIDSVCGGRALC
                     GRCQVEVGEGSFAKHGLASTAAHLDAITATEMRFDEKKGLPAGRRLACQALLRGDVVI
                     DVPQESQVHKQVVRKRAEARAIALDPVLRLHYVEVAEPDMHEARGDFERLKEALEAQW
                     GLSELTIDLPTLRAVQPKLRQGGWRLTAVVHDQKRIIATRPGFHDKAYGIATDIGSTT
                     IAAHLCDLLTGEVLASAGMMNPQIRFGEDLMSRVSYVMMNPGGEKELTETVRGTLNAL
                     MAQLAAEALIDANDIFELVFVGNPVMHHLLLGIDPTELGGAPFALATDSAIELYGTEV
                     GMTAVNDSARVYVLPCIAGHVGADTAGVLLSEGPHLQDETMLVVDVGTNAEIVLGNRH
                     RLLACSSPTGPAFEGAQLTSGQRAAPGAIERVRIDRETLEPRFKIIGTDYWSNEDGFA
                     AATRRIGVTGICGSGIIEAVGEMFLAGLIDADGVIDGGLAPRTPRLVQEGRTWTWVLQ
                     AGEPEIRITQTDIRAIQLAKAALHAGCRLLMDHLGIDKVDRIRLAGAFGSHIDPLYAL
                     LLGLVPDCALAHVSAAGNAAGTGARIALLNVQARREIEALVRRIEKIETAIEPRFQEH
                     FVAAMAIPHKTAATPELAKAITLPVPKRSSTTGDARSRRRAPRA"
BASE COUNT         4053 a         8851 c         8596 g         3718 t
ORIGIN      
        1 agcgccgcct ccacgaggag cgctgcggcc gccacctggt tctcggcgaa gcccgccggc
       61 aggccgggct tcgcggtgtc ggcgccgaac agcttctcga tcaggcccag catcgatcct
      121 cctccaaagg cgctggctca agcccgccag aaggcgggtg caaagagaac cagcacggtg
      181 aaaagctcca gccggccgag cagcatgccg gcagagagca gccatttggc ggtgtccggc
      241 agggccgcga agttcttcgc cggcccgatg aggtcgccga gccccgggcc gacattggcc
      301 agtgccgaga gagcgcccga catcgcggtc aggtagtcca gcccgagaaa gctcagaagg
      361 atgctgagca cggcgaagct cagggcgaag acgaagacga atcccaggat cgagatcgcc
      421 gtctgatccg cgaccggctg gccgttgtag atcggccgga agacgccaga aggctgcagg
      481 aggcgcagca cctgcacgcg ggcaatctgg tagagcacgg cgaagcggaa caccttcatc
      541 ccgcccgtgg tcgagccgct gcagccgccg atgcacatga ggaagaagat gacgccgacg
      601 ggaaacgtgc cccaggcatt gaagtcgctc gcggcatagc ccgtgccggt caccacgctg
      661 accgtcgcga aggccgcgtg gcgcagcccg tcgtgaccga acatcagacc tcgctggacc
      721 agccagaagg cgagaagggc cgtgccgccg gcgaggatgc cgagaaacca gcgcacctgg
      781 acgtcgtgga aaagctggag cttctggcca cgcaggatct ggacgtagag cacgaagggt
      841 atgccgccga cgatcatgaa ggtgatcgcc gtccactcga tcgccgggct ggcgaaatgg
      901 ccgatcgagg cgtcgtaggt ggaatagccg ccggtcgcga tggtcgtcat cgcatgcgcc
      961 gccgcatcga accagctcat cccgaagagc cagtagatcg aggcgcagag cagggagagc
     1021 aggaggtaga tcaaggcgat attggtcgcg atctgcgccg ctcgcggcag gatcttctcc
     1081 gacgaatccg tgctctcggt gcggaagagc tgcatgccgc cgatcgagag catgggcagg
     1141 atggcgatgc ccatgacgat gatgccgatg ccgccgagcc attgcagcat ggcgcgccag
     1201 agcaggatgc cgggcggcgc cgcctccagg ccgacgatca ccgtggagcc ggtcgtggtg
     1261 atgcccgaca tcgcctcgaa gaaggcgccg gcagcaccca gcccgagatc cgagaacgcg
     1321 aacggcaggg cggcgaaggc ggtcgtcgtc acccaggtca gcgtcgtcag gaggaagccc
     1381 tggcgcacgg tgagctcggg cgtgcgcgag acgcccgtgg tcatcaggat caggctgacc
     1441 ccgatgaacg ccgtgcagga ggcgctgagc aggaagacct gccagtcggc atgcccggcg
     1501 atgagatcgg cgagcatcga cggcagcatg gccagggcga ggacgagcag cacgatgccg
     1561 accaccatgg cgaccggccg caggtcgacg atggcccgca gatcgagcct ggaccggctc
     1621 cggcgcacga cccggctggt ctccgaaacc gtcagttcac cgacccgcgg ctgcccggcg
     1681 catcgagcga gaacgccgga atctcgacgt cgaaccagag cccgtcggca cccagcatct
     1741 gataggtccc cgccatgatc cccgtcggcg tccccaaagg cgtgccggag gtgtactcga
     1801 agcgctcgcc gggctccagc atcggctgct cgccgaccac gccgtcgccg cgcacctcgt
     1861 gcagccggcc gcggccgtcc acgatccgcc agtagcggct catgagctgc accggctccg
     1921 agccggtgtt ctcgatgacc acccggtagc cgaagaccca gcggctgtcc gccggctccg
     1981 actgctcctc gacgaagaac gggtcgacgg agacgctgat tgcccgcgtg acctcgctat
     2041 acatgcgaac cgcccctcct ctgctgtgcc ggtcaccagg ccccatggcc gccgaccggc
     2101 gtgaatggca gatgcggact atgtggagag cggatcgggc tgtccagtgt cggccgggcc
     2161 cgaagcgccg gacccggcta gacgctgcag gtagagcacg acggcgaccg ccgtggcgag
     2221 gttgaggctc gacacacctg gccgcatcgg gatcgtgacg attcggctcg ccacccggcg
     2281 cagatgcggg gccaggccat cgcgctccga gccgaagagc agcacggcat cgccgggcag
     2341 cgtgcccggc ccgagcggct cgccgccatc ggcgagggcg atgatcggcc ggccggtcgg
     2401 cggcagctcg tcgggcgctt cgaccggcag ggccatgtgg agcccggccg agccgcggat
     2461 tgccgcggcc gaccaggggt cgaccccgcc tagcgtccag accgccgccg ccccggccgc
     2521 cgcggcgacg cgcacgacgg cccccagatt gcccggatga cgcggccggt cgagcacgac
     2581 gatcgggccg gccgcggtgc tgctccccgg ccataccggg cggtcggcga cagcgatcag
     2641 ccgcgttgcc ggcggctcag gagcgagtgc ggcgaattcg gccgtggtca ccggctcgag
     2701 cagtgccgcg agccgggcct ggacatcggg agccagctcg gccgccagcg ccagcacgct
     2761 tgcgggcgca tcgctgatca ccagcctcgg cacgacaccg aagcgcagcg cgtgcttgac
     2821 cgcgtgcaaa ccctcgagca ccaccatggc cgggtcgcga cgtgcccgat gcaccagcgc
     2881 ctggcgagcg gctgccgtca aatcacggcg tggcgcactt tcgccgacac ccattcgctg
     2941 accagcaccg tgccgagcac caggagcagg atcatcgtca cctgcggcca ggcgagcgtg
     3001 ttgagcgagg cctggagctg cagcccgatt ccgccggcgc cgaccaggcc caggatggtc
     3061 gattcgcgga tgttgatgtc ccagcgaaag acgctgatcc cggcgaaggc cggcaggatc
     3121 tgcgggacga tgccgtaatc cagcacctgc gcacccgagg cgccggtggc gcggaccgcc
     3181 tcgacctggc tctcgtcgat ctcctcgatc gcctcgtaga gcagcttgcc gacgaagccg
     3241 atcgaccgca ggccgatggc gatgatcccc gcgagcagcc cggggccgat catcgagacc
     3301 agcagcagcg cccagatcag cgagttgatc gagcgcgagg cgacgatgat gaagagcgcg
     3361 acgggtcgca ggaaaagggc gctcggcgtg gtgttgcggg cggcgagaaa ggccaccggc
     3421 acggcgatga tgatgccgag cagcgtgccg agcgtggcga tgttgagcgt gtcccagagc
     3481 ggccagagca gcttgtcgat gtaggaccag cgcggcggca gcatccggct gccgatatcg
     3541 gcagcctgcc ggggcgcatc ccagacgaag gcccagatcg tccggccggt catcagcttc
     3601 cagcaccaga cgaagagcat gacaccggcg agccagcccg cccagatcgc ccattggcgg
     3661 cgggtggtgc gccgctgcca gaccggccgc ccgtcgagct ctcgtaccgg cactactgca
     3721 cccacccgcg gacatggctg gagctgtatt ccgccgccat cacgatcagg atgatcagga
     3781 gcaggatggc gccggcgctg tcgtactcgt agcggtcgat ggccgtgttc agcgtggcgc
     3841 cgatgccgcc ggcgccgacg atgccgatca ccgccgattc gcggaagttg atgtccagtc
     3901 gatagaggct gaggccgatc agccgcggca tgacctgtgg ctggatgccg taattgatga
     3961 gctgggggaa cgaggcgccg gtggcgcgca ccgcctcggc ctgggcctcg tcgatgtcct
     4021 cgatgtcctc ggcgagcagc ttggccatga agccgatggt agcgaaggcc agggtgagaa
     4081 agccggcgaa ggggccgaag ccgaacatcg cgacgaagaa gatggcgacg atcacctcct
     4141 ggaaggtgcg gctgagcgcg atgatcgccc ggcagaagag atagatcggc atgggcgcca
     4201 cgttgcgagc agcaccgatg ccgatcggca ccgccaggag cacgccgacc accgtcgagg
     4261 tgacggtcat ggtcaggctc tcggcgaggc cctggctgat gtccttccag cggctgacga
     4321 agtcgggggc gagaaagccc tgggcgaagc gccagccgcg atcgagcccc tcgtagaccc
     4381 gcagccagtt cacctcgacc gtcgagatcg cgagggcgag gtagatcagg agcccgccat
     4441 agacgagcca gcgcgtccgt cggctctgga tgaggaccgg cggtcgccgc cagcggcggc
     4501 cggccgcggc agtgctcatg cgagccccgc cagccgctct tcggggcgct cgccctcggc
     4561 cgcacgacga acgtgcccgc catgcgcggc agccgcgtcc tcggcgtcct cggcgtcctc
     4621 ggcggccgcc gtctgcatcg cggtccagtc ctcggcgccg tagatgcggg tgaggaccgc
     4681 gtcgtcgagc tcggccggcg gcccgtcgaa cacgacctcg ccggcggtga ggccgatgat
     4741 ccggtcgacg aacatcctgg cgagcagcac gtcatggatg ttgatgaccg ccggcagcga
     4801 ccgctcctcg cagatctcgc ggatcagccg catgatctgc cgcgaggtct tgggatcgag
     4861 cgacgccgtg ggctcgtcga tcagcagcag ctccggatcc tgctcgaggg cgcgggcgat
     4921 gccgaccctt tggcgctggc cgccggagag ctcgtcggcg cgcttgtcgg catgctcggc
     4981 aagcccgacc cgctcgagca gcgcgaacgc cttgtcgaca tccgcctgcg ggtagcgacg
     5041 gaggaagctg cgccagaagg gcacgtagcc gagccggccg gagagcacgt tctccatgac
     5101 cgtcagccgc tcgaccagcg cgtattcctg aaagatcatg ccgatccgcc ggcgcacccg
     5161 cctgagatcg gcgccgctca tcgaggtgag ctcgacgtcg ttcagataga ccttgccgcc
     5221 ggtcggctcg accagccggt tgatgcagcg gatcagcgtc gacttgccgg cgcccgacgg
     5281 gccgatcagg ccaacgatct ggcctcggtc gacctccagc gacacgcccc tgagggcatg
     5341 gtcaccggtc ttgtagcgct tggccaggtt gtcgagccgc agcatcagcc tctccgctca
     5401 aagggcccga cccggtgccg ggccctttgc tccatcctcc ggcgagacct actggcaggc
     5461 gtaggaaacg tccattgccg cgtcgatctc acggatcacc gaccattcgt tctggaaggt
     5521 gatcggcacg aaggcttcct gggccggatc ggagttgccg tactcggcga gcagcgaggt
     5581 gccttcccag tcgaagttga agaacgccgc cttgatcttc tcctgcagct ccgggctgag
     5641 gttgtagacg acgccatagc ccgtcgtcgg gaacgggtcg ctctcgtaga tgacgcggat
     5701 ctggtcctga tcgatgacgc cgcgcgcggc catgcggatc atcacctggt cggcgatcgc
     5761 ggcggcgtcg tagtccttgt tgtagacgcc gagcaccgag ttgtcgtgct tgcccgagaa
     5821 cgccgcctcg aagtcgcgac cggcttccat gccgaatttc gagctcaaga gggccgacgg
     5881 tgccttgtag ccggagttcg aggtttccgc ggtgaaggcc atggtgcggc ccttgatgtc
     5941 ctcgaccttc tcgatgccgc tgtcgacatg ggtgatgatc gccatgcgat agccgtacgt
     6001 gccgtcctcg gcggccatca tggcgaacgg ccgaaagccg gcgcaggcga cggccagcgg
     6061 gttggagccg gtgttgaagc cggcgacgtg cagccggccg gcgcgcatcg cctcgatctg
     6121 cgccgcgttc gactgcaccg ggaagaactg caccggcttg cccgtctcct cggagagatg
     6181 atcgaggaaa tcggcccaga caccctcgta gacggccggg tcctcaaccg gcgtgtaggc
     6241 gaagacgagg accggcgggt cgatccagtc ggccggatcg gtcgggatat cggcgatgag
     6301 atcgccatcc gcatcgctgt agcggctgtc gagctggaat tccgcggccg cggtcagcgg
     6361 tgctgcagcg aggagcaggg tcgccagtcc ggcaacgagc ggatggtagg tcatcgagag
     6421 ttctccctga tggtcagctg aagaccagca ttgctactgg cacgcgtagg aaacgccgtt
     6481 ggcctcgtcg atctgccgga tcaccgccca gtcgtccttg aaggtgatcg ggatgaactg
     6541 cgcctcgccc gacttgctga actccgcctc gagcgccgag cccgcccagt cgaagctgaa
     6601 gaaggcctcc tggatcttcg cctgcagctc gggtgtcagg ttgtagacga tgccgtagcc
     6661 ggtcgtcggg aaggtctgcg aggtgtagag cacgacgagc tggtcgtcgc tcacgacccc
     6721 acggtcgatc atccgtgcct tgaccgaatt ggcgatggcg gcggcgtcgt agtccttgtt
     6781 ggcgacaccg agaaccgagt tgtcgtgctt gccggagaag gccgcctcgt agtcggtgcc
     6841 ggcttcgagg ccgaactcgg acttgagcag cgccgatggc gccttgaagc ccgagttcga
     6901 cgtctcggcg gtgaacgcca gggtgcggcc cttgagatcc tcgatcttct cgatgccgga
     6961 gcccggatag gtgatgatct ccatctcgta gccgaagctg ccgtccgccg cggccatcat
     7021 ggtgaacggg cgaaagccgg cgcaggccac ggcgatcggg ttcgagccgg tgttgaagcc
     7081 ggcgacgtgc agtcgaccgg cgcgcatcgc ctcgagctgg gcggcgttcg actgcaccgg
     7141 gaagaactgc accggcttgc cggtcacctc ctccatgtgc gccaggaacc cggcccagac
     7201 ctcggcatag acggccgggt cctcgaccgg cgtgtaggcg aagatcaggg tgtccggatc
     7261 gacgagctgg ctcgcgtcgg tcgggatgtc ggcgatgaga tcgccgtcgg tatcggtgta
     7321 gcgtgggtcg agcgtgaact cggcccgcgc ggcgctgccg agcccgaggg ccaggagcat
     7381 ggccgaggca atgactgtgc gttgcagcat cggtgcagtt ccttcgcaat tgcagcaaaa
     7441 ggctggagga ccagccgagg cttcccggga tcgattttga cagaaggatg acaaaacgga
     7501 agattcgtga ttggtcaacc gccagaaggg cgcatcgacc acaaggagag gcacatcatg
     7561 tcataccgtc agcactgcaa gacgctgctc gccggcgctg ccctggccac cctgggctgg
     7621 ggtgtcgccg aggccgacga gctcaccctc tattgcagcc cccaggagga atggtgccag
     7681 ttgatggtca ccaccttcac ggccgagacc ggcatcgacg tcgccatgac gcgcaagagc
     7741 tcgggcgaga cctatgccca gatcgccgcc gaggcctcga acccgaaggg cgacgtctgg
     7801 tggggcggca cgggcgaccc gcatctgcag gcggccgagg aggggctgac cgaagcctac
     7861 gacacgccga tgctgcccga gctgcacgat tgggccgtca accagtacga ggccgccgac
     7921 ggaaagaccg tcggcatcta tgccggcgcc ctcggcttcg gctacaatac cgagctgctc
     7981 gccgagaagg ggctcgagcc gccggcctgc tgggccgacc tgatcaagcc cgagtacgcg
     8041 ggcgaggtgc agatggccaa cccgaacagc tccggcacgg cctacaccgc cctcgccacc
     8101 ttcgtgcaga tgatgggcga ggacgaggcc ttcgagttcc tcaagaagct gcacgccaac
     8161 atcaaccagt acaccaagtc gggctcggcc ccgatcaagg cggccgggct cggcgagacg
     8221 atggtcggca tcgtcttcca gcacgacgcg gtcacccaga aggtcgccgg cttcccgatc
     8281 gaggtggtga gcccgtgcga agggaccggc tacgagatcg gctcgatgag catcatcaag
     8341 ggcggtccca acccggaatc ggcgcgcaag ttctacgact tcgcgctgcg cgccgacgtc
     8401 cagaccctgg cccgcgacgc ccaggcctac caggtgccgt ccaaccgcaa cgccgaggca
     8461 ccgccggaga gccccgactt ctcgaccatc aagctgatcg actacgactt cgtgacctac
     8521 ggatcgtccg acacgcgcac ccggctgctc gcccgctggg acgccgaggt cggcagcctg
     8581 cccaactgag cagccgatcc ttgtcgcaac agcgaagccc gctcggctac tggctggcca
     8641 ttggctgggc gggcttcctg ctccttccct ggtacgtgca gtcgggcggc ttcttcgatt
     8701 tcgcctggct gctcgcctgg gctgacgccg acgcagcgcc ggccctggcg caggccgtgc
     8761 tccacggccg ctggtggctc ctgccgctcg ccctgccgct ggcgctgccg atcctcctgc
     8821 tcggccgccg gcaggacacg cccgcggcgg cgcggctcct gatcctcgcg ggcgggatcg
     8881 gcctcgcctg gctcgcgctc cagggcttcg ccgtgatcca cagcggctgg ggcttcgaca
     8941 tccttgcccg gacgttcggt ccgctcgagc tgagccagtc gggcatgggt gccggtgcgg
     9001 cgctcaccgc gctcgccctg ctcatgttca cgggcctcgg gatcgcggcg cgcggtgccg
     9061 tcggcggcga ccgcttcgtc gtcggcgcca tcaccctcat cgtcgccctc gtcgcggtct
     9121 tcatcttctt cccggtgctg cgcatcctgg ccggagcgct gcaggattcc gacggcagct
     9181 tcgcccccgg cctgttcgcc gcccgcttca tcgacccgcg catctggggc ctgggttgcg
     9241 tgctcgaagg ctcgcgctgc ggcgtcgcct ggaacacgct cgccctcgcc ctcttcaccg
     9301 ggctgacgac gaccttgctc gggctcgcct ttgcgctcgt cgccacgcgc acggccttcc
     9361 ccgccaagcg ggcgctcagg gcgctgacca tcctgccgat catcaccccg cccttcgtca
     9421 tcagcctcgc catcatcctg ctgttcggcc gctcgggcac ggtgacgcag ttcgtcgccg
     9481 acctcttcgg cgtctccggc ggccgctgga tctacggctt ctggggggtc tggctggcgc
     9541 agatcctgtc cttcacgccg atcgccttcc tcgtgctgat cggcgtggtg gaaggcatca
     9601 gcccctcgct cgaggaggcg gcgcagacgc ttcgggccag tcgctggcag accttcacca
     9661 ccgtctcgct gccgctcatg cggccgggcc tcgccaacgc gttcctgctc ggcttcatcg
     9721 agagcatggc cgatttcggc aatccgctcg tgctcggcgg caatttccag gtgctctcga
     9781 ccgagatctt cttcgccatc gtcggcgccc agttcgaccc cggccgggcc gccatgctgg
     9841 cgatggtgct gctctccttc accctcctgg ccttctgggc gcagcagcgc tggctgggcc
     9901 gcaagctcta cacgacggtc ggcggcaagg gtgattccgg cctcgccgcg agcctgccga
     9961 ccggcctgaa gtggctggtg ctcggggtca ccgtgccctg ggcgatcttc accgccatca
    10021 tgtacgcgat gatcctcttc ggcggctttg tcgagctctg gggccgcaac cacagcttca
    10081 cgctgcgcca ctacgaggcc acgttcggca tcacccaggg ccagttcggc ctcgtctggt
    10141 caggcacggc ctggaacagc ttttggacca ccctgcagat cgccgtcatc gccgccatcc
    10201 cgaccgccct gatcgggctg atcacggcct ggctgctcag ccgccagacc tttcgcggca
    10261 agggcgcctt cgagttcggc accatgctct ccttcgccat ccccggcacg gtgatcggcg
    10321 tcgcctacat catggccttc aacgtgccgc cgatcgagct caccggcacc ggcatgatcc
    10381 tcatcatcgc ctttgtcttc cgcaacatgc cggtgggcgt gcgggccggt gtcgccaccc
    10441 tggcgcagct cgacaagagc ctcgacgagg cctccctgac ccaccgcgcc agcacgctgc
    10501 agaccgtccg ccgcatcctc ctgccgctga tgaagccggc tctgctcgcc gcactggtct
    10561 acagcttcgt ttccgccatg accgccatct cggcggtcgt cttcctggtc tcggcccgct
    10621 acgagctggc cacgacctac atcctcaatc gggtcgagaa cggcgattac ggcatcgcca
    10681 tcgcctacag ctcggtgctc atcgtggtca tgctcatcgt catcggtctc ttccagctcg
    10741 ccatcggcga gcgccgcctc ggccggcgca gcgccgcacc cgccggattc acgggagggg
    10801 tccgatgagc agcgctgcca ccgtcggctt cgagaacgtg accaaggcct tcggcgccgt
    10861 ggtcgccgtg cgcgacgtca gcttcgaagt ggcgcccggc catctcgtca cgctgctcgg
    10921 cccctccggc tgcggcaaga cgacgacgct ccggatgatc gccggcctcg agctgccgac
    10981 cgcggggcgc atccgcatcg gcgagcgcga cgtcactaac ctgccggcca ccgaccgcga
    11041 cgtcgccatg gtcttccagt cctacgccct cttcccgcac atgaccgtgc tggagaatgt
    11101 cggctatggc ctctccgtct ccggccggcc gaaggcggag gtgaaggagc gggcggagac
    11161 gacgctcgag ctggtcgggc tcgagggctt cggtgcccgg ctgccgtccg agctctcggg
    11221 cggccagcag cagagggtcg cggtcgcccg tgccgtggtg ctggaacccg aggtgctgct
    11281 cttcgacgag ccgctctcga acctcgatgc caagctgcgc cgccgggtca gggaggagat
    11341 ccgcgagctg cagcagcggc tcgggctcac cgcggtctac gtcacccatg accaggagga
    11401 ggcgctcgcc atctccgacc gcatcatcgt catgaacaac gcggtgatcg cccaggaagg
    11461 cgtcccccgc gacctctacg aggcgcccgc cgatcggttc gtcgccgact tcatcggcga
    11521 cgccaacgtg ctcgccgtca ccatcggcgc catcgacggc gagcatgccg aggtgaggat
    11581 cggcgaggcc cgccgccgcc tcctgcatcg cggcctcgcc cccgggccgg cccatctcgc
    11641 catccgcccc gagggcatcg tgctgacggc cgaccccgaa gccagcatca tggccggccg
    11701 cgtcaggcgg gcgagctatc tcggcagcca tgtcgagtac gacatcgaga ccgcgctggg
    11761 cgagctcttc gtcacggact ccgacgtcga ccgcattcac ccggtgggaa gcgaggtggg
    11821 gctcggtttc ggcacgcacg gggtcacgct ggtcagggat tgaaacgggc tggtcagcgg
    11881 gcgagcggct cggtgccaag gctgagaatc gccagatagg ccggatagac gaagacgcgg
    11941 cggcgctggc ggccagtgac ctcgcgcacg atgccgagct cttccagatg cctgatcgac
    12001 ttgccgatcg tcggcgtcga gatgccgagc ctagccgcgg cgtcctggat gacgacaatg
    12061 ggcttttgct ggagctgctg gtgcacgcgc agcgccgatg ccgccggccg gccgagctgc
    12121 tcgatgcgct gtctgtcggt ctcgaagagg ttgagtatct ggcgtgccgt ctcggccgcc
    12181 tgtttcgacg tctcggcgac gccggccagg aagaagtcga gccaggcttc ccagtcgcct
    12241 ctttcgcgca cgcgctgcag gagctcgtaa tattccttgc ggtgcgtctt gaagtacagg
    12301 ctgagataga ggatcggctc tttcagaatg ccctccgcga tgagcatgag ggtgatcagc
    12361 aaccgcccaa gtcgcccgtt gccgtccagg aaggggtgga tcgtctcgaa ctgcacgtgc
    12421 gcgagcccgg cccggatcac gaccggcaag gccggttttt cggcatgaat gaacttctcc
    12481 aggtccgaca tgaggtccgg cacgcgctcg aagggtggag gcacgaagtg ggcagtgccg
    12541 ggtcgtgtgc cgccgatcca gttctgcgag cggcggagct cgcccggctg ttgtgtggcg
    12601 ccgcgaccct tggcgagcag aatgccgtgc atctcgcgga tcagacgggt cgagatcggc
    12661 attccttccg ccagacggtc gatgccgcga ttcatggccg cgacgtagct cgagacctcc
    12721 acgacatcgt ccaggggtgc gccgggcgtc tcgtcgagct cgaagagcag gagatcggag
    12781 agcgaggact gggtgccctc gatctgcgac gagagcaggg cttcctttcg gacataggtg
    12841 tagaggaaga gggaggcatc gggcagaatg gtggatacgc catcgagccg cccgagcgct
    12901 tggctcgcgg tctcgaacgc tctgtacagg ccctccattg ccaggggcgg aacgggtggc
    12961 aacggcggcg ggatgaaggc gctgaggggc tcacccgccg tggtggtcgt ctgccactga
    13021 ccgagacggc ggggatcggg tgaggccgac atcgtagcga aaggttcctt tccttgcctc
    13081 gatgcttggg aaaggaaagt ggcatatcct ttccttaagg tcgagccaat gaatggaagg
    13141 gcaacggcat tgcaccttcg gcacgacgcg atcaaccggg cctcagggaa ttcctttgac
    13201 ttctgcactg cagcatgcgc ttatgtggcc accggatatc ggtcctgtgg ctccagccgc
    13261 gtgaagagtg ttcgtcactc tatctgttga gccccctgat aattcctccg gccgcccaga
    13321 tacgcggggt gcgccatcgc caatccggcc gttgcattcg tcgccccttg ggcacgccgc
    13381 tcgcggtcct cctcgaaagt gatctcttga ctcagtttgc tgatttcggc ctcgccgaat
    13441 cccttcttcg tgccctccag agcgaaggct acacttctcc caccccgatc cagaccaagg
    13501 ccgtgatgcc cgtgatggag ggccgtgacc tcctcggcat cgcccagacc ggcaccggca
    13561 agaccgcggc cttcgcgctg ccgatcctgc atcggctctt catcaccgac aagaagcccc
    13621 tgcagccgcg cgccgtgcgc acgctggtgc tggccccgac ccgtgagctc gcggcgcaga
    13681 tcgccgccag cttcgagacc tatggccggc atctgcggcc cgctgtcacc gtcgtcgtcg
    13741 gcggcgtcaa gcacaacccc caggtccgcg ccctcgcacg cggcgtcgac gtgctcgtcg
    13801 ccacgccggg ccggctgctc gaccatctgc agggcggcac ggtccgtctc gaccagaccg
    13861 agatcgtcgt cctcgacgag gccgaccaca tgctcgacct cggcttcctc gtccccatcc
    13921 gccgcatcct ggcgaagctg ccgaagcgcc gccagacgct gttcttctcg gccaccatgc
    13981 cgaaggacat cggcgagctc gcccgcgaca tgctgagcga gcccatcgag gtcagcgtcg
    14041 ccccggtcgc gaccaccgtc gagcgggtcg accagtcggt ctatctgatc gacgcggcgg
    14101 acaagcgaac actcctggcc gagctcctgg cgaagcccga gttcagccgg gcgctggtct
    14161 tcacccggac caagcacggc gccgacaagg tcgcccgcca cctcgaggcg gccggcatcc
    14221 cggctgccgc gatccacggc aacaagtcgc agagccagcg cgagcgggcg ctcgccgcct
    14281 tcaagtccgg acgctcgcgc atcctggtcg cgaccgacat cgcggcgcgc ggcatcgacg
    14341 tcgacgccgt cagccacgtc gtcaatttcg agctgcccga cgtgcccgag gcttatgtcc
    14401 accgcatcgg ccgcaccgcc cgtgccgggg ccgagggcgt cgccatctcg ttctgcgacg
    14461 ccgccgagcg gcgcaacctg cgcgacatcg agcggctgac ccggcagacc ctgccggtca
    14521 tcgaccgccg ccggccgggc agcgccaacc gaacgccggc cccggccgag cgtggccggc
    14581 cgcagcgccc gcagcagcag cgccgcagcc cctcccgccg cgccgcctga acggcacgac
    14641 cgacacagac caaaacgccc ggctccggcc gggcgtcttg cgttccggcc catgcttgac
    14701 gccggcgcgt cggcgggcgc aagtgcccgt catcgagctg tcacgggaca gtcgccaatc
    14761 gggtgacgcc gcttgagccc tctcgtcttt gccgtggtgt tgctggcggc ggtcctccat
    14821 gcgagctgga acgcgctcgt caagtcgggt ggcgacccct atctccggct ggccatcgtc
    14881 aatctcaccc acaccgtggc cgtcctgccg ctgctgccgt tcgtgcccct gccggacgcg
    14941 gccgcctggc cctggctgct cggctcggtc ttcacccatc tcgcctatta cctgttcctc
    15001 gccgccggct atcgggtcgg cgatctgagc caaatctatc ccattgcccg gggtgtggcg
    15061 ccgccgctcg tggcgctggg cgcgctcctc gtcgccggcg aggtgctgag cccgaccggc
    15121 agcttcgcga tcgccctggt ctgcctcggc atctggtggg tggccggcgg tggccccaca
    15181 aaccggcggc cgctgctgct ggcgctgggc accggcggga ccatcgccgc ctataccgtg
    15241 tgcgacggca tgggtgggcg agccagcggc gacgtgctgg cctatatcgt ctggctcttc
    15301 ttcctcgacg gctggccctt cgcgctgctc gtggggctgc gccgcggccg gcatctggcg
    15361 cggaccctgc cccaggcctg gaaaccggcg gtaggcggcg ggctgatgtc gatctgcgcc
    15421 tacgggctgg tcatctgggc gatgagcatc gcccccatgg cctatgtctc cgccttgcgc
    15481 gagaccagcg tgctcctcgc cgccggcatc ggcgccttcc tgctcggcga gcccttcggc
    15541 cggcgccgca tgctcgccgc cggtgtcgtc gtcatcggcg tcgtcctgct gcagatcagc
    15601 cgccagggct gaagcccggc atcgtgccgg tcagtgatca gagcggtgca ggatcgcctc
    15661 ggcgacgccc gtctcgtagc tcatggcggc agcggtcagg gcggggcggc cgtcgggggc
    15721 catggcgatc aggcccttgc gccgcagagc accgaacacc cccggattgc ccagccccgt
    15781 cgcatccttc gcccgcacca cggcggaccc gacatggaaa tggtcgccat gcgcatgtgg
    15841 cagccggttg agcgccacgc tgccatccgc cagctcctcg ctgacgtcgc cgccgctgcg
    15901 cgccacctcc tgcaggatgg ccagcgtctt caactgcagg gcgttcagct tcgaggggtt
    15961 ggcgcggctc gacatggcgg agctccggca agggcgggat cgacaggcac ccctacatcg
    16021 ccgcccggca ggcgcacgtc cagatcaaac ggcggtgcag ccgattcggc ctcagaaccg
    16081 cgacagggtc agcccgccgt cgacggcgat cggctggccg gccgtgtagg gcagctcgcc
    16141 ggcggcacag gccacgaccg ccttcgccac ctcctcgggc tcgccccagc gcggcatcgg
    16201 cgtgccgtcc gcggcgaaga aggcgtcata gcgttccctc gaaggtgcgg tcatctcggt
    16261 ccggatgatg cccgggcgga tctcgtagac gccgatgccc aagggggcca ggcgcagcgc
    16321 gaagagcttc gtcaccatgg cgagcccggc cttggagacg cagtattccc cgcggttgag
    16381 cgaggccgcg gtggcattgg acgagctgac gatgacgatc gagcgatgca caggtgacgg
    16441 tgcctgctcg atcaggcgcc tggcgaaggc ctgggtcagg aagaacgtgc cgcgcgtgtt
    16501 gacccgctgg cacctgtccc agctttccgg cgtgagctcc aggagatcgc cgcgctgcag
    16561 gaccgacacc ccggcattgt cgaccaggca ggccacgggg ccgaaggcga aggccgcatc
    16621 gagcaggccg gcgtggctct cgaggtcgcc gatgtcggca agggcggcga ccgcctgcgc
    16681 accggccgcc cgcaccgcct cggccgtggc ctcgagctcg gcgcccggct cgagatcgtt
    16741 gagcacgaga tcgaagccgc ggccggcgag gccgagggcg atggcccggc cgatgccccg
    16801 ccgggcgccg gtgacgatgg cgacgggacg ttccgacatg actggcctcc tcgatggtga
    16861 aaggggtggc cggctgggcg gccggtcaat gcagggcggt gatcgactgc gtgaccagaa
    16921 tgccgcccga gatcaagagc aggatcagga cgatccgctt gaagagcgcc tcgccggcgg
    16981 ccgagtacag cctgaggccg atccaggtgc cgagcacggt caccggcagg caccaggcga
    17041 ccgcgcggac gaccgagccg tcgatctgcc cggccagcag catgccgaga ccggcgagcg
    17101 ccaagatgat caggttgaag ggctggtagg tcatgcgctg gcgggtgctg ggcccgccgc
    17161 gcagctgcag ccagatcaga ggcagcggcc cggaaaggcc ggcgaagccg ccgaggaagc
    17221 cgccgccgcc gccgatcgcc atgtcggccc gccagccgcc ccaggtgccg acggaccagt
    17281 tgcggatggc gctgagctgc acgagggcgt aggcgacgag aaaaatgccg accgtgaccc
    17341 ggagcaggga cgccgaggcg acctgcagcg ccatgatgcc gaagggcagg cccagcacac
    17401 cgcccgcgag atagggtgag acgccgcgcc agtcgaagga ctggcgcagg cggtggagcg
    17461 ccatgagctg ggcgatgacc gaggtgaaca cgatcatcgg tggcacgctg gtcgccggca
    17521 gggccacgaa ccagaagccg gacgcgacga ggccggtgcc gaagccggcg aagccgctga
    17581 cgaaaccggc aatgagggcg ccgccgagca gcagcaccag attgaacgcc tcgagctcga
    17641 aaatgcgcgc acctcggaga aaacccgtcc ccgaatagcc ggctcggcga gcttcaccca
    17701 gccttttcga cgacgcccgc cctggcaggg tggcgtgttg accagcacct cgtcatccgg
    17761 ttccctgccc ctggcggcac aggccgcctg ccccctcgga ggagctcctg cgatgtcatc
    17821 caacacgggc acggacgaga ccctgaaggc acggacccgg ctcgaggccg agctcgccga
    17881 gctgcgcgcg acctcggtgg cgaccgccag tgatcgccgg ccggtcgagc tcgaccaggc
    17941 ctcggtcggc cggctgtcca ggatggatgc catgcagatc caggccatgg cccatgccgc
    18001 ggatcgccag cgccagcagc gcatccgtcg ggtggaggca gcgctcgagc gcctggccga
    18061 cggcgactat ggctggtgcg tgcgctgtgg cgaggcgatc gcgtcaggtc ggctcgaggc
    18121 cgatcccgcg gtgcccacct gcctcgcctg cgccgtctga gcgactctgt ccggctacag
    18181 ttcgccgaag cggcacgaga gaacgcattg cgtgccgggc cagcagcagc aggcaggcgg
    18241 cgaaggtcgc agcttccacg agcgcctcga ccgctggcag gcggacctga ccgagcatgc
    18301 ccgcgaggtc cagcccgacc gacagggcgc cgcccacgac cagggtcaca acgaagcgct
    18361 gcgccagccg ccgctcgtgg cgatcgtgcg gtgcatgcag cgccaccagg atggcgccga
    18421 gcaccatgag cgcgagggcc gcgaggatcg ccagcttggc gagctggatc gcattcagcc
    18481 cgggcagggg cgaggggccg atgactcggg ccagtccggg agccagcgcc agatgcgcat
    18541 cgcccagcgc cacccagacg agaagcgtca gggcgaaccc cagaaccagc ggcaccggcc
    18601 gcgggcaaat gatgtgcggc acggcccccg gccctggcgc cgtgaacggc gctggccgag
    18661 gtgccgtgca cgtcctgtgc gacaccgagc gtttcctctt ccccacggga cgttttcgtc
    18721 ctcgtccgga catcctaagc cggccgccct taaaaaagcc ctacagcggc gcaccgcccc
    18781 gctgtcgcaa aaccggggtg gcgcgtcgcc ggcgacaaag ccggccgggc gccgacgacc
    18841 tatctgctgc ctgtagtggc gaggacggcc tccggcaggg acatccgacg atgagcgacg
    18901 aagccgaact gaacctgcgc gagctcgacg acgaggagct ggtcaagcag atccaggacg
    18961 acctctacga cggcctcagg gacgaggtgg tcgagggtgt cgacatcctg ctcgagcggg
    19021 gctgggagcc ctatcgggtg ctgaccgagg ccctggtcgg cggcatggcg atcgtcggcg
    19081 tggatttccg cgacggcatc ctcttcgtgc ccgaggtgct gctcgccgcc aactcgatga
    19141 agggcggcat ggcggtgctc cgcccgctgc tcgccgagac cggtgcgccg accgtcggca
    19201 agctggtgat cggcacggtc aagggcgaca tccacgacat cggcaagaac ctcgtctcga
    19261 tgatgatgga gggtgccggc ttcgaggtga tcgacctcgg catcaattgc gacgccgata
    19321 agtacctgaa ggcgctggac gagcacaagc ccgacttcct cgggatgtcc gccctcctca
    19381 ccaccaccat gccctacatg aaggtggtga tcgacacgct gatcgagcag ggcatccgcg
    19441 acgactacgt cgtcctggtc ggcggcgccc cgctcaacga gcagttcgcg aaggccgtcg
    19501 gggccgacgc ctattgccgc gatgcctcgg tggcggtcga caccgcccag gccttcatgg
    19561 cgcgcaagca caaccagcgc gcatcggcct gaagcatgcc gcccgaggcg ctcaccgtca
    19621 tcgcctgcgg ggccctcgcc cgcgagatca cgcggctgat cgctttcagc aagctgcact
    19681 gggtgaccct ggcctgcctg ccggcgacgc tgcacaaccg cccggagcgg attcccgagg
    19741 ccgtgcgcgg cgccattcat cgcgcccgcg agcggggcgc gtcgcggatc gtcgtcgcct
    19801 atgccgattg cggcacgggc ggcctgctcg accaggtgct cgccgaggaa ggcgtcgttc
    19861 gcctcgaggg tgcccactgc tacgccgcct atgccggcca ggaagccttc gccgccctcg
    19921 ccgaagccga gcccggcacc ttctacctca ccgatttcct cgcccgccag ttcgacacgc
    19981 tcgtggtcga ggggctcggc ctcgatcgcc atcccgagct cagggatgcc tatttcggca
    20041 actacacgag gctcgtcctt ctggcccaga gcgacgacgc ggcgctgacg gcgaaggccg
    20101 aagccgcggc ccgcaggctg ggcctcgcct tcgagcgcgt cttcaccggc atgggcgagc
    20161 tcgccacctt cgtccaggcc gccgccgacg ggaccatcca tggcgcaact gaccattctc
    20221 tactggcgcg acatcccggc ccaggtgatc gccagggcgg gccgccggac ggccaagcgc
    20281 cagctcccgg cgcgcttcga gcaggcgatc gaccgggcag cgatgcaggc gaagctcacc
    20341 gggacggaag cctatctcga gcagtggcgg aaatctgacc cgaccgattg cggcgatgac
    20401 ctcgaggcgg cggccgaaga ggcggcggcc gcgctcgaag ccgccttcac gccagagcaa
    20461 ttgcgtgact acgtcatgaa tggcggccac actccccggg cctgaagcgg ctgcgcctgg
    20521 gaacctcgat caccgtcggc cggttccatc gactgcgctt cgcgacatga caagggggac
    20581 acgggcacat gaacgagctt tcgaactggt cgcggacgac ctggatcgtg gtcggcgcac
    20641 tcctggtcat cgcggtgctg ggctggggtt ttgctctcga ccgggaccgg cggctggagg
    20701 agcatgcatc gcagatcacg gtgctggagg gtgagcagag cgcgctcggc cagcgtttcg
    20761 cggagctcga gacgacgctc gccgacgagc gccaggcagc cggcgacctc gccgccctga
    20821 acgagcagat ggcgacagca caggccgagc tggagacggt cgaggcggcg aagaccgagg
    20881 ccgaggcggc ggtcgaggcc gcgcagggca ccgtggccga ggccgagcag cgtgtgaacg
    20941 acctcaccga cgaggccaaa gaggccgagg agcggctggc cggcctcgcg gaggagatcg
    21001 gcacggccga ggccacgctc gccgagcgca gcgaggaggt cacccgctcg gagacggccc
    21061 tcgcccgggt cggcgagctg gaggcccggg tgaccgagct ttcggcccag gtggcggccg
    21121 ccgagagccg tctagccgcc ctcaacgagg aggcgggcag cgtcgagagc acgctggtgg
    21181 cccgccagtc ggagctcgac gaggtcacga cccggctcgc cgaggcgaag tccctggccg
    21241 agcagcaggc gggcgaggcg cgtggcctcg aagcccggat caccgagctc accggcgccg
    21301 tggcgggcga ggagtcgcgg ctcgccgccc tgcaggccga gcgcgagcag acggaggctt
    21361 cgctcgagac gctcgccgcg gggctcgaat cggccgaggc cgagctcgcg accgcccagg
    21421 cgaagcatga agccaccgtc gccgagacca ccggcctgga gcagcgccgc gccgacctgg
    21481 tgaacgagat cggcaccgcg gaggcgcggc tgcaggaaat ccagggccag gccggcacag
    21541 ccgaggccga gctcgctgcg cgccagggcg ccgtcgagga ggccgaggcc cggctcgccg
    21601 agacccaggc ggcgacggcc gagcagcagg cgcagctcga ggggctcgag gcggaggtgc
    21661 ggcagctgca ggagcaggcg gcagcggcgc gccgggagct gcagcaggcg cagaccgccg
    21721 agcaggcgct cagggagcag atcacggccc tcgaggacca gcggaccggg atcgagaccc
    21781 tggtgggcga gacgcagagc cagctcgagg cgcagcaggg cgagctcgag gcgctccgcc
    21841 gccagctcac caccgagcag ccccaggcgg cgcctaccca ggcggcgccg gcccagtagc
    21901 accacgccac cgccacccac gccgcaaatc ggcgcgtcca tggcgcgccg acgcgggtgg
    21961 cgggtggcgg gctctggcat cagggcggag agcctgaccc ggagcctggc acccatgacc
    22021 gagaccgtcg tcgcttccgc cacgaagacc gtgcgtatcg gcttcgagcg ccccttctgc
    22081 gtcatcggcg agcgcatcaa cccgaccggc cgcaggaagc tcgcggccga gatggcggcg
    22141 ggcgacttct cgaccgtcga ggcggatacc ctggcccagg tcgcggccgg ggcgagcatg
    22201 ctcgacgtca atgccggaat cccgctcgcc gacgagccgg cgatcctggc ccggaccatc
    22261 gagctggtgc agtccctgac cgatctgccg ctctcgatcg acagctcgat cgtggcggcg
    22321 ctcgaggccg gcctcgccgt ctataagggc cggcccctgg tcaactcggt gaccggcgag
    22381 gaggagcggc tcgaggcggt gctgccgctc atccggaaat acgactgtgc ggtggtcgcc
    22441 atctccaatg acgagacggg catcagcgag gacccggacg tccgcttcgc ggtcgcgaag
    22501 cggatcgtcg agcgcgcgca ggaccacggc atcgagccct gcgacatcgt cgtcgatccc
    22561 ctggtcatgc cgatcggggc actcgggcag gccggcaact cggcctttcg cctgatccgc
    22621 cggctgcgcg aggagctgca tgtcaacacc acctgcgggg cctcgaacgt cagcttcggc
    22681 ctgcccaacc gccacgggct caatgccgcc ttcctctgca tggcggccgg tgccggcatg
    22741 acctcggcga tcctcaaccc gctccacgcc gaggagatga cggccgtcat gggtgccgac
    22801 gtcctgctcg gcaaggaccg ggattgccga agctggctca cccgctttcg cgagccgacc
    22861 gctgaaggca cgagccgcaa cgacaggcgc cggcggcgcg cctgaccatg acggcctgcc
    22921 tcccatgacc gatccattgg tcgtcttcac cccgtccggc aagcgcggcc gtttccctgt
    22981 gggcacgccg gtcctcacgg cggcccgcca gctcggcgtc gacatcgaca gcgtgtgcgg
    23041 cggccgggcg ctttgcggcc gctgccaggt cgaggtcggc gagggcagct tcgccaagca
    23101 cggcctcgcc agcacggccg cccatctcga cgccatcacg gcgaccgaga tgcgcttcga
    23161 cgagaagaag ggcctgccgg ccggccgccg cctcgcctgc caggccctgc tgcgtggcga
    23221 cgtggtcatc gacgtcccgc aggaaagcca ggtccacaag caggtcgtgc ggaagcgcgc
    23281 cgaggcccgc gccatcgccc tcgatcccgt cctccgcctg cactatgtcg aggtcgccga
    23341 gcccgacatg cacgaggcca ggggcgattt cgagcggctg aaggaggcgc tcgaggcgca
    23401 atggggcctc tccgagctca ccatcgacct gccgacgctc cgggccgtgc agcccaagct
    23461 caggcagggc ggctggcgcc tcaccgccgt ggtgcatgac caaaagcgca tcatcgccac
    23521 ccggccgggc ttccacgaca aggcctacgg gatcgccacc gatatcggct cgaccaccat
    23581 cgccgcccat ctctgcgacc tgctgaccgg cgaggtgctg gcctcggccg gcatgatgaa
    23641 cccgcagatc cgcttcggcg aggacctgat gtcgcgggtc tcgtacgtca tgatgaaccc
    23701 gggcggcgag aaggagctga ccgagaccgt gcgcggcacg ctgaacgcgc tcatggcgca
    23761 gctcgccgcc gaggcgctga tcgacgccaa cgacatcttc gagctggtct tcgtcggcaa
    23821 cccggtcatg caccatctgc tgctcggcat cgacccgacc gagctcggcg gggccccctt
    23881 cgcgctcgcc acggacagcg caatcgagct ctacggcacc gaggtcggca tgacggcggt
    23941 caacgattcc gcccgggtct acgtcctgcc ctgcatcgcc ggccatgtcg gcgccgacac
    24001 cgccggcgtg ctgctctccg aggggccgca tctgcaggat gagacgatgc tggtggtcga
    24061 tgtcggcacc aatgccgaga tcgtgctggg caaccgccac cgcctgctcg cctgctcgtc
    24121 gcccaccggg ccggccttcg agggggcgca gctcacctcc ggccaaaggg ccgcacccgg
    24181 ggcgatcgag cgggtgcgca tcgatcggga gacgctcgag ccgcgcttca agatcatcgg
    24241 caccgactac tggtccaacg aggacggctt cgccgccgcc accaggcgga tcggcgtcac
    24301 cggcatctgc ggctccggca tcatcgaggc ggtgggcgag atgttcctcg ccggcctgat
    24361 cgacgccgac ggggtgatcg acggcggcct cgccccgcgc acccctcgcc tcgtccagga
    24421 aggccgcacc tggacctggg tgctccaggc cggcgagccc gagatccgca tcacccagac
    24481 cgacatccgt gccatccagc tcgccaaggc cgccctccat gccggctgcc gcctgctcat
    24541 ggaccacctc ggcatcgaca aggtcgatcg catccgcctc gccggcgcct tcggcagcca
    24601 catcgacccg ctctacgccc tcctcctcgg cctcgtcccc gactgtgccc tcgcccacgt
    24661 ctccgccgcc ggcaacgccg ccggcaccgg ggcgcgcatc gccctcctca acgtccaggc
    24721 ccggcgcgag atcgaagcac tggtccgccg catcgagaag atcgagacgg cgatcgagcc
    24781 ccgcttccag gagcacttcg tcgccgccat ggccatcccg cacaagaccg ccgcgacccc
    24841 cgagctggcc aaggccataa cgctgccggt ccccaagcga tccagcacga ccggcgacgc
    24901 ccgatcacgc cgccgagcac cacgggcctg atataggatt ttttgcctat ttcggctcaa
    24961 ggcgcaagtg gcttccgggt tccttcggca attccccgct cccgccgtcg gacgctgtcc
    25021 cattcgggcg cgactcgggc ctttcgatcg tgtccctggt ggtggtgtag ctggcggcgg
    25081 tcatcggcgg ttcttcggta aggtctggtt gcgcagccaa tccgaaccgg agcaccgaac
    25141 cgatgatcga cgagatgatg gcgcttcagg gtcttctgga gaagacgtcc gacgctgacc
    25201 tcttgcgcga gatgctgg
//
LOCUS       DAOTFI010000029        24980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2321_length_24980_cov_7.294403, whole genome shotgun sequence.
ACCESSION   DAOTFI010000029 DAOTFI010000000
VERSION     DAOTFI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 24980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24980
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_24980_cov_7.294403"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="P5558_04455"
     CDS             complement(<1..368)
                     /locus_tag="P5558_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HRY23594.1"
                     /translation="MTKLVVEAGPFTFDARFEEAAAPRTCAAFKQAMPFEGQIVHVRW
                     SGEGVWIPLGDRDFGVAYENHTSHPAPGQIILYPGGISETEILLAYGGVDFSSKMGQL
                     AGNHFITLTSGHENLMKLGNL"
     gene            complement(388..1083)
                     /locus_tag="P5558_04460"
     CDS             complement(388..1083)
                     /locus_tag="P5558_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY23595.1"
                     /translation="MSIRWDWSGFFDYLFNGFLLRGAFTTLWLTLAAIAGGLVLGFAL
                     AMLRLSPIRPLSLLAQGYIWIFRGTPLLVQLIIIYTGLPQFGLRFSVVQAALLGLILN
                     EAAYLAEIVRGGILGVPQGQSNAGKALGLSPAQVMLFVVMPQATRIIIPALGNSVNGL
                     LKTTSVTSVISMEELLRSTQILIQEKFLVLELFTVAALYYLLMTSLWDVVQRRIEARF
                     GRAYSSIRAPEPY"
     gene            complement(1181..2023)
                     /locus_tag="P5558_04465"
     CDS             complement(1181..2023)
                     /locus_tag="P5558_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23596.1"
                     /translation="MASRIETSGPALAVLLLATTIAGSAMAQDCVATVPDSALVEPGK
                     LTMSTNPTIPPMQFVDSEGNLKGMRITLGEELAKRLCLEPEYIRIEFSAMIPGLQAGR
                     WDVINTGIFWTAERAGMMQMIPYESQAISISVPHGNPEGIASVEDLAGKKVAVEIGGF
                     EEAKIKQLNEELQAKGIEAMEILTFDNFAIAYQALQAGQADATVSIDATAAEFAARGD
                     FDRALHGLFPTPVALAMSNVEVAEAVRDALNSMMEDGFYQSLMDQYGLLANTEPFIIR
                     GPND"
     gene            complement(2100..3008)
                     /locus_tag="P5558_04470"
     CDS             complement(2100..3008)
                     /locus_tag="P5558_04470"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY23597.1"
                     /translation="MPRARGTMTVLVTPFTEDGGAVDLPALRRLVDWQIEEGIHGLVP
                     LGSTGEFLAIGREERQAIVEACVETARGRVPVYIGTGAEVTGDAVELAREAERLGADG
                     VMIIPPFYSVPTEDELFEHYRRIGEAISIPIMVYNNPAAANVDIRPETLARLARIDTV
                     RSVKESTLEVTRVRDIIELCGDRIDVFAGVLGYESFWLGAVGWIAVCSNLIPKESARL
                     FELAVDESRRDEALALYRRILPIVRWVGGPRYVQATKAGFGLMGASMGPPRPPRLPLP
                     GEQRVAFGADLKRLGLLDSLSRLHAR"
     gene            3152..6100
                     /locus_tag="P5558_04475"
     CDS             3152..6100
                     /locus_tag="P5558_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY23598.1"
                     /translation="MRLNGPATLAFTFDGETVPARPGDTVASAMAAAGRMALRRTGRD
                     DASNDARRGLWCGMGACFDCAVTIDGRIGERACLAKVAGGEVVRSAMPAGTTLDPLAP
                     LAPPPEGASLPERHCDILVIGAGPAGLAAALAARRLGASVLVLDERAASGGQYYKQPA
                     TPGAVPVDRQGRDGAALIRSAVEAGVEIVQNAQVWGAFAADDVLALVAGRAVRFRPQR
                     LILATGAYERPAPIPGWTLPGVMTTGAAQTLERAHGVAPGRRVVVGGNGPLNFQLASA
                     LTRMGVAVAALAEAAPRPGAGALAPLLQALRLAPDLMAEGLRYRWQLWRRGVPLHWST
                     AAVAARGDGRVEEVDLAPLDASGRGRPEATLTIACDTLCLGHGFIASTEIARALGCAM
                     RLDDRHLGTAAVATDDAGLTSIAGVYAVGDGAVVAGARVALERGRLAGDAAARSLGLG
                     AALADDLAPARRRLARATGFQQALWTLFSAPPVRLDLVPDATILCRCEGLDFGRVRAE
                     IAAGWDTAATLKRRLRLGMGSCQGRNCTATAARLLAEATGRARHVEEGFAPRLPVKPF
                     PAAALALEKPEWGGHRRAGSPDLARGAEATAAEAPGRADVVVIGGGVVGACLAFYLAE
                     AGADVLVVERDDINLQASGANAGSLHVQLLSFDFGAKAEAGGGPAAATLPLGPWSIGL
                     WQELAAIAGGDFEIRVTGGLMVADTPRGMAFLEAKVALERQHGIDSRMIDRDELRRLA
                     PALSDHLLGAEHCPGEGKINPLTATYRVMEAAMAQGARLLRRADVKGLERRPGGWAVR
                     TSRGAIEAGIVINAAGPWARLVGAMAGVETPVWSAPLQMIVTERAPKLVEPLVAHADR
                     HLSLKQLASGGIVIGGAWTAAYDQGRNMNVTLRQSIEGNLWVAGSVLPQLAGLRVLRS
                     WAGMNVNIDGAPIVGEAPGAPGFFTCVTSNGYTLAPAVARITADLVLTGRTARDIRPY
                     TLARFTEKVA"
     gene            6097..6825
                     /locus_tag="P5558_04480"
     CDS             6097..6825
                     /locus_tag="P5558_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRY23599.1"
                     /translation="MIRIEDVSKWYGTFQVLKDCSTSVERGEVVVICGPSGSGKSTLI
                     KCVNGLEPFDKGSIEVAGMRLENPRTDQTKLRTRIGMVFQNFELFPHLTIMGNLTLAQ
                     RKAIGRPRAAAEEKGRMLLERVGLADQAMKYPGQLSGGQQQRVAIARALAMDPLAMLF
                     DEPTSALDPEMIHEVLDVMVELADEGMTMVCVTHEMGFARKVAHRVVFMDQGEIVEDC
                     PKDAFFNDTRTPRAQQFLARILAH"
     gene            6836..7864
                     /locus_tag="P5558_04485"
     CDS             6836..7864
                     /locus_tag="P5558_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23600.1"
                     /translation="MTAFAQAVTGPRPAAELGLTLMHEHILCDLRRPEDRGPADRHPA
                     ITCENRFETDYFQNRNPLNMFLDEDDAAIAGLVRFRDAGGGTVVELTIGGIEPQPLRL
                     RAIAEASGVAVIAGAGFYVDAYLDDETRALAPDAIEAVIQGQLEDGLWGTDVRAGLIG
                     EIGCSWPLVPVERRMLEAAARVSARTGAALTIHPGRHEEAPREIAGIVLAAGGDPART
                     VIGHMDRTLFDRERVIELLRLGFVLEWDFFGIETSQYWMAGTDLDLPTDYMRLDLLRD
                     LVDRGYRDQLCLSHDICTRTRLCAHGGHGYRHLPAHVVPLMQRRGFTESEIEALLVET
                     PRRLLAYL"
     gene            7883..8551
                     /locus_tag="P5558_04490"
     CDS             7883..8551
                     /locus_tag="P5558_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY23601.1"
                     /translation="MLDTPLKDPPDRDRGSRAHRAADTIREMILARRLEGGDILSEEK
                     LAQLLQISRTPVREALFLLQSEGLIQKQVSQPFRVRLVSNKEYFQSMRLRELLEGEAV
                     GMAIDRLEPAELDAREAEMESLRHDPAASAEAHWSADEALHGMIADACDNDVMGRMIR
                     TLRVTTRLYELSGLPSRFRPDTEEHLVIIRALRARDATRARAAMAGHIRSLTTDVLET
                     MARL"
     gene            8563..9840
                     /locus_tag="P5558_04495"
     CDS             8563..9840
                     /locus_tag="P5558_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY23602.1"
                     /translation="MKDTATSRLADLVRAEGQGPAAVRAALAEQMAMAGCAVETLAYL
                     PGEVPMVDEFAHASVAADAGETVTIGRLAAAGAGGGGGRSLLLFAHPDTEPHQPDPGW
                     RSAPFEPTERNGRLHGWGVADDLAGCAMLSAALLELRAAGLRPAGDVTLVAAPSKRHR
                     RGIGAALHAGLRADAALYLHPAESGRGLGEIKAFAPGQLEFALTVEGVPPETAEPAHT
                     AFAHRGVNPIGKAMAILAALDALDRDRGERIHHPRLETAIGRSTNLMVTHIAAGEADV
                     TTRLPRTCRLRAAVSLVPDEKLDAVMGEIEAAVARAASADPWLAGHPPRIDWLAGVSA
                     AETADTDPFFRTVAGAIEAAGTTPGVNPLHTSSDIRNPIVQAGIPTLGIGPLCGGLTM
                     AGGVDEWVDLADYRRSVAVVAAIIADWCGTLRA"
     gene            complement(9844..11295)
                     /locus_tag="P5558_04500"
     CDS             complement(9844..11295)
                     /locus_tag="P5558_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY23603.1"
                     /translation="MTEGKNLLFVFSDEHSRDITGAYGNSIVKTPNLDAMAASGTRFA
                     NPYVNCPICVPSRASLATGRYVHEIGCWDNAHPYAGDPPGWGQALIDEGHDVTAIGKL
                     HYRSEADPVGFSEQIETLNVVDGVGDLLGMIRRPPAARGNVGDLAGEAGRGESTYTQY
                     DRRICAHAESWLERRAAAPTGKPWMLFVGFVLPHFPLVAPEEFYDLYDPALMPLPRLT
                     GPGERPDHPVLRALRDCMNYEDFFDEGKVRKAVTAYYGMVSFLDHHIGRLVRKLEETG
                     LSGDTRVIYTSDHGDNLGNRGFWGKSVMYDDSTAVPLIMTGADIAPGRVVETPVSLVD
                     LAPTFMEALGAKPMAADLPGRSLFAIADEPDDAERVVFSEYHAVGAIAGMFMVRKGRW
                     KLVHYAGHEPQLFDLVADPQEANDRAEDIACAAVRADLEAELRKICDPDEVNRRAFAD
                     QEARIAAHGGAEAIKARGDFGYTPAPGQTPVFG"
     gene            complement(11321..12223)
                     /locus_tag="P5558_04505"
     CDS             complement(11321..12223)
                     /locus_tag="P5558_04505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23604.1"
                     /translation="MSKEAVGYIGLGHMGSGMVRNLLANGWPVVVHDQRTQAVQAMVK
                     EGATAAGSAREVAESCRVVLSSLPDPAAVDAAALGEGGIIEGIQKGGVYIDLSSIDPN
                     TSRKVHAALAEKGARMLDCPVGKGPPEAASGDLALMIGGDADLIEEMRPLLETLGSSI
                     HHCGPAGAGAAAKLINNLVSCSLAALSSEAVVLGAKAGVDLDTLCTIMASTAADNRHL
                     RITVMPRTLDGIFEPRFKLALAHKDLGLACRMGLELGVPTPLGSAARIVHDMAMGMGL
                     AEEDQGACIKPLEKAAGVEARRRK"
     gene            complement(12252..13127)
                     /locus_tag="P5558_04510"
     CDS             complement(12252..13127)
                     /locus_tag="P5558_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY23605.1"
                     /translation="MSSAIERAKSGTGAVPRPLQVSPEARRRRLLNWLAVAILLFVMI
                     PFFWMVQMSFRPNDDIFGYELLFSPTVEHYTALWHGNFPTSFLNSVLSSGISTILALI
                     VGTPAAYSLARWEFRGKEQVGLWILATRMAPPIAFTIPFFLAYRYLGLSDTILGLVLI
                     YLTFNLALVIWMMQTFFATVPKSLEEAAWIDGCSVWGAFLRITLPLTAPGLAATAVLC
                     FIFSWNDFFYALILTRTNAQTAPVAIVNFIQYEGYEWGKIAAGGTLVMMPVVVFTMLV
                     RTYLVRGLTAGGIKD"
     gene            complement(13120..14028)
                     /locus_tag="P5558_04515"
     CDS             complement(13120..14028)
                     /locus_tag="P5558_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY23606.1"
                     /translation="MASAAITSWDRQRRRAFFWGLFPALAVLAVITVAPAVYLVVTSL
                     TPLNLTNPVQTGLDFSDPLRNYREAIADYRFRNSVWVQVKLSLATVVAQVLIGLGLAL
                     LLNARSKLLEAIRTAFLIPMVLPPIVVALIWKIIYSPDVSPMHRLLEFVGFPVRSLIT
                     QADTALWAIAVADTWEWFPFTMLIILASLQMVPDEPLEAAKIDGATSYQSFWYVVLPY
                     IQPAIIVAALFRLIDSVKAFPLIYVLTNGGPGQVTEVTNYFAFVQAFNFSYWGYASAI
                     ATMLVAGVFLLSWLLRRLDQAGQRDE"
     gene            complement(14100..15443)
                     /locus_tag="P5558_04520"
     CDS             complement(14100..15443)
                     /locus_tag="P5558_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY23607.1"
                     /translation="MSTIWRMSRRSLLGSTAALGSTLILPGKAPAQGGAKPFAGTTLN
                     ISSWSAPYAKWLQEYLPEFTELTGIEVNYDTPGFAVYNQQVDLELSTGGSAYDVANIT
                     FIYTSRWIGAGWFTPLDDFYNDPNKTPDEFDAADFLNGTQLPMRDAEGRTYGFSWVAD
                     AMHAGASRFDIMEEAGFGMPKSFDELMAMVPEIHKVKGVSAFTAENHHGWIFPPIMQA
                     FGGNVFVDPPHNIMPALDTPEVHEASRWYVDLQNNYGPDGWLAQTYDQTVNVLKQGRA
                     NYCLFNQPFLMQMGAPDSKTKETCAFAMYPEGPKGKFPGLAVHGWGIPTGARNKDASW
                     EFIKWAVGKDLMMRMLEEKGYGAVTRRSVIESPKFKEVNMVNGYDVGDIYLRTIGLAE
                     QGHMAYRTVHVYPQANAQINKAIEMIASGQATPEEAWRQAQENAIADIRRAGVDL"
     gene            15685..16437
                     /locus_tag="P5558_04525"
     CDS             15685..16437
                     /locus_tag="P5558_04525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23608.1"
                     /translation="MTTNRSLAGKVVLVTGASSGIGAAIAVGCAAAGATLVLAARRAA
                     ELKTVAAEARRHDVEVLAVPTDVTDPQAVDALMRATLERHGGLDIVMGIAGGNTARGA
                     VAEIDAAEWIACLNLNVTGGFLTARAAVPLLEAKGGGRIVLMGSGMGHRGNPGHSAYC
                     AGKAAQWMLVRCLAEELKEKNISVNELIPGPVITPATTAEMAEGGPTSGFSSPGEWRK
                     QPEDVVPMALFLATCPEPGPTAQSFSLMRRQG"
     gene            16444..17325
                     /locus_tag="P5558_04530"
     CDS             16444..17325
                     /locus_tag="P5558_04530"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23609.1"
                     /translation="MGETIGYIGLGVMGLPFARHLKAAGHDVVVYDRDAAARARAAEA
                     GLRVAAGIAEAARDAGIVFTCLPNPAAVQEVHAEIGRPGQLAIDNSTVGPDLARRLHV
                     ELAARGAAYVECPMLGGVGEAEAGQLFLIVSGDAAAVERALPLALLAARDHRVVGGPG
                     AASLFKTVQNGLGLVQMTAIAEALALVAAGGGDLDRFIDVVGAGGGMAATPLFRTKAP
                     LMRDAGAKAVGQFYIGAKDAGLAAALAQAQGLDLPLFAASDSAWREGMAAGLATADVS
                     AIARVIEARTKTKIAGS"
     gene            17330..18061
                     /locus_tag="P5558_04535"
     CDS             17330..18061
                     /locus_tag="P5558_04535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRY23610.1"
                     /translation="MREREIEIDGPDGRLPVFVTRPEEGGPHPTVILYMDALGIREEL
                     RDMARRIGTVGYSVMLPNLFWREGGASFDPALLPTHGPDPEMMRLNHATTNAMVQADT
                     ALLLDHAAADSAARDPVGVIGYCMGGRHAMGAAGTFPDRVAAMVSLHGGFQVTDRPDS
                     AHLLIPRMQAECYFGFADGDPLSPDEHQRVMEAACEANGVEARFEVYEGAHHGFTFPT
                     RFCYHKPSAERAWERVFALFRRRLG"
     gene            complement(18058..18966)
                     /locus_tag="P5558_04540"
     CDS             complement(18058..18966)
                     /locus_tag="P5558_04540"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23611.1"
                     /translation="MAKRIGFVGIGTIGKPMALNIRKGGYELAVFDLDQSAVADLIAA
                     GATGKGSPAAVAEASDIVITMVPDAPDVEKAALGAGGIIEGLAPGALYIDMSTVDPAT
                     TRKVGAAMAARHIRMVDAPVGRTVDNAYAGTLSIMVGGEAADVEEAMPVLKCMGTDIT
                     HCGPLGNGHAVKLVNNYISGGILALLSEGLTMGVKSGLELETILEVVGATFATSGIMT
                     KMLPARAFHGDFSPGFKTTLSHKDIRLGLGLATAAGLDAPVGHGVYETLSRTIAAGYG
                     DDDFTSMLRVNEAKAGVEVRLKGQGD"
     gene            19058..20584
                     /locus_tag="P5558_04545"
     CDS             19058..20584
                     /locus_tag="P5558_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRY23612.1"
                     /translation="MRCVFLLFDSLNRHLLGPYGGQRIETPNFDRLAKRSITFDNHYV
                     GSMPCMPARRDMQTGRLSFLHRSWGPLEPFDNSWPLLLAEKGVYSHLITDHAHYFEDG
                     GATYHTRYDTFEFIRGQEGDPWKAMVQPPWERLREKYHERQYGEGNRTYFRNNMVNRE
                     YIRSEADFPSVQCFAAGLEFLETNKGADNWLLQLETFDPHEPFTAPERFKDAYSTGWN
                     GPIRDWPRYARVDELPEECEELRANYYALVALCDHLLGTVLDFFDANDMWKDTVLIMT
                     TDHGYLLGEHDFWAKNRMTMYEEVAHIPLFVHHPDHADKAGQRRTALTQNMDMAATFV
                     ELFGAERPAEMQAESLLPHLAGDLEGREAVLFGYFGGAVNLADGHYTYHRYPPDLATQ
                     EIYQYTLMPTHIWTRFSVEELRPATLSEPLAFTKGVPVLKVPMTETTPIYNNYGPGCL
                     LEDETRLYDIVADPGQERPLVEPELEARLAGIMARLMAGNDAPPEAFARLRLEPAPAG
                     "
     gene            20640..21998
                     /locus_tag="P5558_04550"
     CDS             20640..21998
                     /locus_tag="P5558_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY23613.1"
                     /translation="MKSFRNAILTPTRRATLGLGAGAAATLIGAPYVLRRARAQGDFD
                     WQRFAGEHLEVFLSKNPRSALLQEHEAEFVELTGITVGSEQVPEQQHRQKQVIEFTSG
                     ATSFDVTMTAYHVQKRLFAKGGWLEDLRPFLDNPELTPPDYDFADFSAGAVAYATQAD
                     GRMDTVPFNIDPWIVYWNQDLFAEAGIAYPDTFDGMLEAAKAIHAPDKGVYGVVSRGL
                     KNANTPVWTGLMLGWDMEAVDAEGNLTTTSPEAIAAAELYKTLNADYAPEGVVGFNWN
                     ECQTSFMQGTVGMWIDGLGFAKPLEDSATSKVAGKVGYGIFPAGPTARHSPLFGSGLG
                     VSAFSPRKEAAFLYCAWATNKVNQIRMLQSGAGAPCRNSAIFDPEVLASLAFPEQWAE
                     TLVESARIARPGLPVIIPVSEFRDIYGVALSNMISGADPADELARATEQFRPVLEKSE
                     RA"
     gene            22021..>22907
                     /locus_tag="P5558_04555"
     CDS             22021..>22907
                     /locus_tag="P5558_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY23614.1"
                     /translation="MSVEAPAGIALGRSGNPAVRRWQYLLFAAPALVAVFLVIVFPWA
                     FTVFMSMHDWGVVGSRTYVGADNFLRLLSDSRFIESIGHTLYFTALAIVLPVVLGVFA
                     AVVFHRSFPMRGVLRAIFIMPMMATPVAIALVWTMMFHPQLGVLNYLLTSVGLPPSLW
                     VYSPTTVIPALVLVEVWHWTPLVMLIVLGGLASLPTEPYESALIDGASAWQMFRHITL
                     PLVMPFIMVAVIIRAIDALKTFDTIFVITQGGPGTASETINIFLYLQAFAFYDIGYAS
                     AVVVVFFVIIVVFSLILLWA"
     assembly_gap    22908..23007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23105..23986
                     /locus_tag="P5558_04560"
     CDS             23105..23986
                     /locus_tag="P5558_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY23615.1"
                     /translation="MNATGPLAQVKGAFSVSAKRGFEVLFGKLAFAFAVLVIVSPAIL
                     VFLWMLSLSLKTEVENTAFPPVFIPGTLTLQNFYAVFESSPFALYTLNSIIVSGTATG
                     LALLVGVPAGYGIAKARANGIAVLILIARMTPGLSYLIPLFIMFRFLGLTNSLTPIII
                     THLVITVPIVVWIMIGFFENLPRELEESALIDGASLWQAFWRIALPLSRPGIVVAAIL
                     AFIFSWNNFIFGVVLAGRETRTLPVAVYNVLTFEQISWGPLAAAALVVTLPVLVLTVV
                     IQKQIVGGLTSGAVKGG"
     gene            24023..>24980
                     /locus_tag="P5558_04565"
     CDS             24023..>24980
                     /locus_tag="P5558_04565"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY23616.1"
                     /translation="MTLNAPSGAPDHDAIIVGAGFAGLYMLHKLRGQGLRAHVFEAAG
                     GVGGTWWWNRYPGARCDVPSVEYCYSFDEGLQQDWSWSERYATQAEILAYAEHVADRF
                     DLRRDITFEARVETAILDEAEAIWEVETTKGRASARFLILATGNLSVPNWPAIPGLDR
                     FRGRVLHTGAWPHEPVDLEGQRVAVIGTGSSAVQSIPAIAREAARVHVFQRTPAYAVP
                     AHNHPLTEEQRAAIKADYPALRAAALTRRNYINFPQNQQKAMSVDAATRRAEYEARWA
                     TGGLSFNGAFVDQLIDRRANDEAAAFVREKIQKIVLDPETAAR"
BASE COUNT         3731 a         8919 c         8399 g         3831 t
ORIGIN      
        1 aggttgccga gcttcatcag gttctcgtgg cccgaggtca gggtgatgaa atggttgccg
       61 gcgagctggc ccatcttcga cgagaaatcg acgccgccat aggcgagcag gatctccgtc
      121 tcgctgatcc cgcccggata gaggatgatc tggccgggtg ccggatggct cgtgtggttc
      181 tcataagcga cgccgaagtc gcggtcgccg agcggaatcc agaccccctc gccgctccag
      241 cggacatgca cgatctggcc ctcgaacggc atggcctgct tgaaggcggc gcaggtcctg
      301 ggcgccgccg cctcctcgaa tcgggcatcg aaggtgaacg ggccggcctc gacgacgagc
      361 ttggtcatga tcggttcttt cctgctttca atagggctcg ggcgcgcgga tggagctgta
      421 ggcgcggccg aagcgggcct cgatgcgccg ctggacgacg tcccagagcg aggtcatgag
      481 gaggtagtag agggcggcca cggtgaagag ctcgagcacg aggaactttt cctggatgag
      541 gatctgcgtg ctgcgcagga gctcctccat cgagatcacc gaggtgaccg acgttgtctt
      601 caagaggccg ttcacgctgt tgccgagggc cgggatgatg atccgcgtcg cctgcggcat
      661 gacgacgaag agcatgactt gcgccgggct caggccgagc gccttgccgg cgttgctctg
      721 cccctgcggc acgccgagga tgccgccgcg cacgatctcg gcgagatagg ccgcttcgtt
      781 gaggatcagg cccagaaggg cggcctggac caccgagaag cgcagcccga actgcggcag
      841 ccccgtgtag atgatgatga gctgcaccag gagcggcgtg ccgcggaaga tccagatgta
      901 gccctgcgcc agcaggctga gcgggcggat cggcgacaac cggagcatcg ccagcgcgaa
      961 gccgagaacg aggccgccgg cgatggcggc gagcgtcagc cagagcgtgg tgaaggcgcc
     1021 ccggagcaga aagccgttga agagataatc gaagaagccg ctccagtccc agcggatcga
     1081 catggcttcg gcctcgtcgt tggagtgtca cccgcgagcg gtgcggccgg ggagaggagc
     1141 cgggcccggc cgacggcggc cgggcccgcg cggcccagga tcagtcgttc gggccacgga
     1201 tgatgaaggg ctcggtgttg gcgagcagcc cgtactggtc catcaacgac tggtagaaac
     1261 catcctccat catgctgttg agcgcgtcgc ggacggcctc ggccacctcc acgttgctca
     1321 tcgccagcgc caccggcgtc gggaagaggc cgtgcagcgc ccggtcgaag tcgccgcggg
     1381 cggcgaactc ggccgcggtc gcgtcgatgc tcaccgtggc gtcggcctgc ccggcctgca
     1441 gcgcctggta ggcgatggcg aagttgtcga aggtgaggat ctccatggcc tcgatgccct
     1501 tggcctgcag ctcctcgttg agctgcttga tcttggcctc ctcgaagccg ccgatctcga
     1561 ccgcgacctt cttgccggcg agatcctcga cgctggcgat gccctccggg ttgccgtgcg
     1621 gcacggagat gctgatcgcc tgcgattcgt aagggatcat ctgcatcatc ccggcccgct
     1681 cggcggtcca gaagatgccg gtgttgatca cgtcccagcg gccggcctgg aggcccggga
     1741 tcatcgccga gaactcgatg cggatgtact cgggctcgag gcagagccgc ttcgccagct
     1801 cctcgccgag cgtgatccgc atgcccttga ggttgccctc gctgtcgacg aactgcatcg
     1861 gcgggatggt cgggttggtc gacatggtga gcttgccggg ctcgacgagc gcactgtccg
     1921 gcacggtcgc cacgcagtcc tgggccattg cggatcccgc gatggtcgtc gccaggagca
     1981 gcaccgccag ggccgggccg gaagtctcga ttctcgatgc catgatcgtt tcctctccac
     2041 gcttcttggt tgtcggcagt cttcttggtt gtcggcagtc ttcggatgat ggccgcccgc
     2101 tagcgggcgt gcaatcggct gagcgaatcc aggaggccga gccgcttcag gtcggcgccg
     2161 aacgcgactc tttgctcgcc aggcaagggc aggcgcggcg gccggggcgg ccccatcgag
     2221 gcgcccatga ggccgaagcc cgccttggtc gcctggacgt agcgcggccc gccgacccag
     2281 cgcacgatcg gcaggatgcg ccggtagagc gccagcgcct cgtcgcgccg gctctcgtcg
     2341 accgcgagct cgaagaggcg ggcggactcc ttcgggatca ggttggagca gacggcgatc
     2401 cagccaacgg caccgagcca gaacgattcg tagcccagga caccggcgaa gacgtcgatg
     2461 cggtcgccgc agagctcgat gatgtcgcgc acgcgcgtca cctcgagggt cgattccttg
     2521 accgagcgga cagtgtcgat ccgggcgagc cgggccagcg tctccggccg gatgtcgaca
     2581 ttcgccgccg ccgggttgtt gtagaccatg atcgggatcg agatcgcctc gccgatccgg
     2641 cggtagtgct cgaagagctc gtcctcggtc ggcacgctgt agaagggcgg gatgatcatg
     2701 acgccatcgg cacccagccg ctcggcctcg cgcgccagct ccaccgcatc gccggtcacc
     2761 tcggcacccg tgccgatata gaccggcacc cggccgcgcg cggtctcgac gcaggcctcg
     2821 acgatcgcct gccgctcctc gcggccgatc gccaggaact cgccggtgct gccgagcggg
     2881 acgaggccgt gaatgccctc ctcgatctgc cagtccacca gccggcgcag ggccggcaga
     2941 tcgaccgcgc cgccgtcctc ggtgaagggt gtcaccagca ccgtcatggt gccacgcgct
     3001 cgcggcatga ggcttctctc cctcggctcg cccgggacca tatcgaaaaa attatctctg
     3061 tatactggaa aaatatcggt ctaggctgcg ctggtcgccc ctttcctgct ggccagccgt
     3121 ccatgcaacc ggccccgaac cctgccgacc gatgcgcctg aacggccccg ccacgctcgc
     3181 cttcaccttc gatggcgaga ccgtgcccgc ccgcccgggc gacacggtgg catcggccat
     3241 ggccgccgcc ggccgcatgg cgctgcgccg caccggccgg gacgacgcct cgaacgatgc
     3301 ccggcgcggc ctctggtgcg gcatgggtgc ctgcttcgac tgcgcggtca cgatcgacgg
     3361 ccggatcggc gagcgcgcct gcctcgccaa ggtcgccggc ggcgaggtgg tgcgctcggc
     3421 catgccggcc ggcacgacac tggacccgct cgcccccctg gcgccgccgc ccgagggcgc
     3481 cagcctgccc gagcgccact gcgacatcct ggtgatcggc gccggcccgg ccggcctcgc
     3541 cgccgccctc gccgcccgcc ggctcggcgc ctcggtcctc gtgctcgacg agcgggcggc
     3601 gagcggcggc cagtactaca agcagccggc gacacccggc gccgtccccg tcgaccggca
     3661 gggccgcgac ggtgcggcgc tgatccgctc cgccgtcgag gccggtgtcg agatcgtcca
     3721 gaacgcccag gtctggggcg cctttgctgc cgatgacgtg ctcgctctcg tcgccggccg
     3781 ggcggtgcgc tttcgcccgc agcgactgat cctggcgacg ggcgcctatg agcggccggc
     3841 gccgatcccg ggctggacgc tgccgggcgt catgaccacg ggtgccgcgc agacgctcga
     3901 gcgcgcgcac ggcgtggcac cgggccggcg ggtcgtcgtc ggcggcaacg gtcccttgaa
     3961 cttccagctc gcttcggccc tgacgaggat gggcgtcgcc gtcgcggccc tggccgaggc
     4021 ggcgccgcgc ccgggtgccg gagccctcgc gccgctgctg caggcgctcc gcctcgcccc
     4081 cgacctgatg gccgaggggc tccgctatcg ctggcagctc tggcgccggg gcgtgccgct
     4141 ccactggtcg acggccgccg tggcggcgcg cggcgatggc cgggtcgagg aggtcgacct
     4201 cgcaccgctc gatgcctcgg gccggggccg gcccgaggcg accctgacca tcgcctgcga
     4261 cacgctctgc ctcggccacg gcttcatcgc ctcgaccgag atcgcccggg cgctgggctg
     4321 tgccatgcgg ctcgacgacc gtcatctcgg cacggccgcc gtggccaccg acgatgcggg
     4381 tctgaccagc atcgcgggcg tctacgccgt cggcgacggt gccgtggtcg ccggtgcccg
     4441 tgtcgccctg gagcgcggcc ggctcgccgg cgatgcggcg gcgcgcagcc tcggcctcgg
     4501 cgccgccttg gccgacgacc tcgccccggc ccgccgccgc ctcgcccggg cgaccggctt
     4561 ccagcaggcg ctctggacgc tctttagcgc cccgccggtg cggctcgacc tcgtgccgga
     4621 cgcaaccatc ctctgccgct gcgagggact cgacttcggc cgggtcaggg ccgagatcgc
     4681 cgccggctgg gacacggccg ccacgctcaa gcgccgcctc aggctcggca tgggcagttg
     4741 ccaggggcgc aactgcacgg ccacggcggc ccggctcctg gccgaggcga ccggccgtgc
     4801 ccggcatgtc gaggagggct tcgccccgcg cctgccggtc aagcccttcc cggcggcggc
     4861 cctggccctg gagaagccgg aatggggcgg ccaccgccgc gcgggctcac ccgacctcgc
     4921 ccgcggggcc gaggcaaccg ccgccgaggc acccggacgc gccgatgtcg tggtcatcgg
     4981 cggcggggtg gtgggcgcct gcctcgcctt ctatctcgcc gaggccggcg ccgacgtgct
     5041 cgtcgtcgag cgcgacgaca tcaacctgca ggcctcgggt gcgaatgccg gcagcctgca
     5101 cgtccagctc ctctccttcg acttcggcgc caaggccgag gcgggcggcg ggccggcggc
     5161 ggcgaccctg ccgctcggcc cctggtcgat cggcctctgg caggagctcg ccgccatcgc
     5221 cggcggcgac ttcgagatca gggtcaccgg cggcctcatg gtcgccgaca cgccccgcgg
     5281 catggccttc ctcgaggcca aggtggcgct cgagcggcag cacggcatcg actcccggat
     5341 gatcgacagg gacgagctcc gccggctcgc cccggccctt tccgatcacc tgctcggcgc
     5401 cgagcactgc cccggggagg gcaagatcaa cccgctcacc gccacctatc gggtcatgga
     5461 ggcggccatg gcgcagggtg cccgcctgct ccgccgggcc gatgtcaaag gcctcgagcg
     5521 ccggccgggc ggctgggccg tccgcacctc gcgcggcgcc atcgaggccg gcatcgtgat
     5581 caatgccgcc ggcccctggg cgcgcctcgt cggtgccatg gcgggcgtgg aaacgcccgt
     5641 ctggagcgcg cccctgcaga tgatcgtgac cgagcgcgca cccaagctgg tcgagccgct
     5701 cgtggcgcat gccgaccggc atctgagcct gaagcagctc gcctcgggcg gcatcgtcat
     5761 cggcggtgcc tggaccgccg cctatgacca agggcgcaac atgaacgtca cgctgcgcca
     5821 gagcatcgag ggcaatctct gggtcgccgg ctcggtcctg ccgcagctcg ccggcctgcg
     5881 cgtgctcaga agctgggccg gcatgaacgt caacatcgac ggtgcgccca tcgtcggcga
     5941 ggcgccgggt gctcccggct tcttcacctg cgtcacctcc aacggctaca cgctggcccc
     6001 ggccgtcgcc cggatcaccg ccgacctcgt gctcaccggc cggaccgcgc gcgacatccg
     6061 cccctacacg ctcgcccgct tcacggagaa ggtcgcatga tccgcatcga ggacgtctcg
     6121 aaatggtacg gcaccttcca ggtgctcaag gactgctcga cctcggtcga gcgcggcgag
     6181 gtcgtcgtga tctgcggccc ctcgggctcg ggcaagtcga cgctgatcaa gtgcgtcaac
     6241 gggctcgagc ccttcgacaa gggcagcatc gaggtcgccg ggatgcgcct cgagaacccg
     6301 aggaccgacc agacgaagct caggaccagg atcggcatgg tcttccagaa cttcgagctc
     6361 ttcccgcatc tcaccatcat gggcaacctc accctggccc agcgcaaggc catcggccgg
     6421 ccgcgcgccg ccgccgagga gaagggccgg atgctgctcg agcgggtcgg cctcgccgac
     6481 caggccatga agtacccggg ccagctctcg ggcggccagc agcagcgcgt cgccatcgcc
     6541 cgcgccctcg ccatggaccc gctggcgatg ctcttcgacg agccgacctc cgccctcgac
     6601 ccggagatga tccacgaggt gctcgacgtc atggtcgagc tcgccgacga gggcatgacc
     6661 atggtctgcg tcacccacga gatgggcttc gcccgcaagg tcgcccatcg cgtcgtcttc
     6721 atggaccagg gcgagatcgt cgaggactgc ccgaaggacg cgttcttcaa cgacacccga
     6781 accccgaggg cgcagcagtt cctcgcccgc atcctggcgc attgaggggg gtgcggtgac
     6841 cgccttcgcc caggccgtga ccggccccag gccggcggcc gagctcggcc tcacgctgat
     6901 gcacgagcac atcctctgcg acctgcgccg acccgaggac cgcgggcccg ccgatcgcca
     6961 cccggcgatc acatgcgaga accgcttcga gaccgactac ttccagaacc gcaacccttt
     7021 gaacatgttc ctcgacgagg acgatgccgc catcgccggg ctcgtccgct tcagggacgc
     7081 cggcggcggc acggtcgtcg agctcaccat cggcggcatc gagccccagc ccttgcgctt
     7141 gcgggccatc gccgaggcga gcggcgtcgc cgtcatcgcc ggcgccggct tctacgtcga
     7201 cgcctatctc gatgacgaga cccgcgccct cgcaccggac gcgatcgagg ccgtgatcca
     7261 gggccagctc gaggacgggc tctggggcac cgacgtgcgt gccgggctga tcggcgagat
     7321 cggctgctcc tggcccctgg tcccggtcga gcggcgcatg ctcgaggcgg cggcgcgcgt
     7381 gagtgccagg acgggtgccg ccctcaccat ccacccgggc cggcacgagg aggcgccccg
     7441 cgagatcgcc gggatcgtgc tcgccgccgg cggcgatccg gcgcgcaccg tcatcggcca
     7501 catggaccgg accctcttcg accgcgagcg ggtgatcgag ctcctgcgcc tgggcttcgt
     7561 gctggagtgg gacttcttcg gcatcgagac ctcgcagtac tggatggccg gcaccgatct
     7621 cgacctgccg accgactaca tgcggctcga cctgctgcgc gacctcgtcg accgcggcta
     7681 ccgggaccag ctctgcctgt cgcacgacat ctgcacgcgc acccggctct gcgcccatgg
     7741 cggccacggc taccggcatc tgccggccca tgtcgtgccg ctgatgcagc ggcgcggctt
     7801 caccgaaagc gagatcgagg cgctcctggt cgagaccccc aggcgcctgc tcgcctacct
     7861 ctgaacagcg gacgacgacg cgatgctcga cacgcccctc aaggacccgc cggatcgcga
     7921 tcgcggcagc cgcgcgcatc gcgccgccga caccatccgc gagatgatcc tcgcccggcg
     7981 cctcgagggc ggcgacatcc tgtcggagga gaagcttgcg caactgctgc agatctcgcg
     8041 cacgccggtg cgcgaggccc tcttcctcct gcagagcgag gggctgatcc agaagcaggt
     8101 gagccagccc ttccgcgtcc gcctcgtctc caacaaggag tatttccaga gcatgcggct
     8161 gcgcgagctt ttggagggcg aggccgtggg catggcgatc gaccggctcg agcccgccga
     8221 gctcgatgcc cgcgaggccg agatggaaag cctgcggcac gatccggccg catcggcgga
     8281 agcgcactgg agcgccgacg aggcgctgca cgggatgatc gcggatgcct gcgacaacga
     8341 cgtgatgggg cgcatgatcc gcacgcttcg ggtgaccacc cgcctctacg agctctcggg
     8401 cctgccgtcg cgctttcgcc ccgacaccga ggagcacctg gtgatcatcc gcgcccttcg
     8461 cgcccgcgat gcaacccgtg cccgcgccgc catggccggc cacatccgca gcctgacgac
     8521 ggacgtgctc gagaccatgg cgcggctttg aggagggcgg acgtgaaaga cacagcgacg
     8581 agccggctcg ccgatctggt gcgcgccgaa ggacaggggc cggcggccgt ccgcgcggcg
     8641 ctggcggagc agatggcgat ggccggctgc gcggtcgaga ctctcgcgta cctgcccggc
     8701 gaggtgccga tggtcgacga gttcgcccat gcctcggtcg ctgccgacgc cggggagacc
     8761 gtcacgatcg gccgcctcgc ggctgctggc gctggcgggg gagggggtcg cagcctgctc
     8821 ctcttcgccc accccgacac ggagccgcac cagcccgatc cgggctggcg cagcgcgccg
     8881 ttcgagccga ccgaacggaa cggccggctg cacggctggg gcgtggccga cgacctcgcc
     8941 ggctgcgcca tgctgtcggc tgccctgctc gagctgcgcg ccgccgggct ccgcccggcc
     9001 ggcgacgtca cgctcgtcgc cgccccgagc aagaggcacc gccgtggcat cggcgccgcc
     9061 ctccatgccg ggctccgggc cgacgccgcc ctctacctcc atccggccga atcggggcgc
     9121 gggctcggcg agatcaaggc gtttgcaccc ggccagctcg aattcgccct gaccgtcgag
     9181 ggcgtgccgc ccgagacggc ggagccggcg cacaccgcct tcgcccatcg gggcgtgaac
     9241 ccgatcggca aggccatggc gatcctggcg gcgctggacg cgctcgaccg cgatcgtggc
     9301 gagcgcatcc accacccgcg cctggagacc gccatcggcc gctcgaccaa cctcatggtg
     9361 acccacatcg ccgccggcga ggccgacgtg acgacccgcc tgccgcgcac ctgccgcctt
     9421 cgcgccgccg tctcgctggt gccggacgag aagctcgatg ccgtgatggg cgagatcgag
     9481 gcagcggtgg cacgggccgc ctcggccgac ccctggctcg ccggccatcc gcccaggatc
     9541 gactggctcg ccggcgtcag cgccgccgag acggcggaca cggacccctt ctttcgcacc
     9601 gtcgcaggcg ccatcgaggc cgccggcacg acgcccggcg tcaacccgct gcacacctcg
     9661 agcgacatca ggaacccgat cgtccaggcc ggcatcccga ccctcggcat cggccccctc
     9721 tgcggcggcc tcaccatggc cggcggggtc gacgaatggg tcgacctcgc cgactaccgc
     9781 cgcagcgtcg ccgtggtggc ggccatcatc gccgactggt gcggcaccct tcgcgcctga
     9841 gcatcagccg aagaccggcg tctgccccgg cgccggggtg tagccgaaat cgccccgggc
     9901 cttgatcgcc tcggccccgc cgtgggctgc gatccgcgcc tcctggtcgg cgaaggcgcg
     9961 ccggttcacc tcgtccgggt cgcagatctt ccgcagctcc gcctcgaggt cggcgcggac
    10021 ggcggcacag gcgatatcct cggcccggtc gttcgcctcc tgggggtcgg ccacgaggtc
    10081 gaagagctgg ggctcgtggc cggcgtagtg gaccagcttc cagcggccct tccggaccat
    10141 gaacatgccg gcgatggcac ccaccgcgtg atactcgctg aagaccacgc gctccgcatc
    10201 gtccggctcg tcggcgatgg cgaagagcga gcggccgggc aggtccgccg ccatcggctt
    10261 cgcgccgagc gcctccatga aggtgggggc gagatcgacg aggctgaccg gcgtctcgac
    10321 cacccggccc ggggcgatgt ccgccccggt catgatcagc ggcacggcgg tggaatcgtc
    10381 gtacatcacc gacttccccc agaagccgcg attgccgaga ttgtcgccgt gatcggaggt
    10441 gtagatgacc cgcgtgtcgc ccgaaaggcc ggtctcctcg agcttcctga ccagccggcc
    10501 gatgtggtgg tcgaggaacg agaccatgcc ataataggcg gtgaccgcct tcctcacctt
    10561 cccctcgtcg aagaagtcct cgtagttcat gcagtcgcgc aaggcccgca gcaccgggtg
    10621 gtccggccgc tcgcccgggc cggtgagcct cggcagcggc atcagggccg ggtcgtagag
    10681 gtcgtagaac tcctccggag ccacgagcgg gaaatgcggc aggacgaagc cgacgaagag
    10741 catccagggc ttgcccgttg gcgcggccgc ccggcgctcc agccagctct cggcatgggc
    10801 gcaaatgcga cggtcgtact gggtgtaggt cgactcaccc cggcccgcct cgcccgccag
    10861 gtccccgaca ttgccacgcg cggccggcgg ccggcggatc atgccgagca ggtcgccgac
    10921 gccgtcgacc acgttcaggg tctcgatctg ctcggagaag ccgaccgggt ccgcctcgct
    10981 ccggtagtgc agcttgccga tggcggtgac gtcgtggccc tcgtcgatca gcgcctgccc
    11041 ccagccgggg ggatccccgg cataggggtg ggcgttgtcc cagcagccga tctcgtgaac
    11101 gtagcggccc gtcgcgaggc tggccctgga cggcacgcag atcgggcagt tgacgtatgg
    11161 attggcgaag cgcgtgcccg aggccgccat cgcgtcgaga ttgggcgtct tgacgatcga
    11221 attgccataa gcgcccgtga tgtcacgact gtgctcgtcc gaaaagacga agagcagatt
    11281 ctttccttcg gtcaccgaat cacctttcgc tgaaaaaacg tcactttcgg cgtctggcct
    11341 cgacaccggc ggccttctcg agcggcttga tgcacgcccc ctggtcctcc tcggcgaggc
    11401 ccatgcccat cgccatgtcg tgcacgatgc gcgccgccga gcccaagggc gtcggcacgc
    11461 cgagctcgag gcccatgcgg caggcgaggc ccaggtcctt gtgggcgagg gcgagcttga
    11521 agcgcggctc gaagatgccg tccagggtgc gcggcatgac ggtgatgcgc agatgccggt
    11581 tgtcggccgc cgtgctcgcc atgatggtgc agagcgtgtc gaggtcgacg ccggccttgg
    11641 cccccagcac cacggcctcg gagctgagcg ccgccagcga gcacgagacg aggttgttga
    11701 tcagcttggc ggcagccccg gcgccggccg ggccgcaatg atggatgctc gagcccaggg
    11761 tctcgagcag cggccgcatc tcctcgatca ggtcggcgtc gccgccgatc atcagcgcca
    11821 ggtcgccgga ggccgcctcg ggcgggccct tgccgaccgg gcagtcgagc atcctggccc
    11881 ccttctcggc cagggctgcg tgcaccttcc tcgacgtgtt gggatcgatc gagctgaggt
    11941 cgatgtagac gccgcccttc tggatgccct cgatgatgcc gccctcgccc agggccgcgg
    12001 catcgaccgc cgccgggtcg ggcaggctgg agaggacgac gcggcagctt tcggccacct
    12061 cgcgggccga ccccgcggcc gtggccccct ccttgaccat ggcctggacc gcctgggtcc
    12121 gctggtcgtg gaccaccacc ggccagccat tggcgagcag gttccggacc atgcccgagc
    12181 ccatgtgccc gaggccgata tagcccactg cctccttgct catgctttcc tctttccgtc
    12241 tcgctgaagg gtcagtcctt gatcccgcca gccgtgaggc cgcggaccag ataggtgcgc
    12301 accagcatcg tgaagacgac gaccggcatc atcaccagcg tgccgccggc ggcgatcttg
    12361 ccccactcgt agccctcgta ctggatgaag ttgacgatcg cgaccggggc ggtctgggcg
    12421 ttcgtccgcg tcaggatcag ggcatagaag aagtcgttcc aggagaagat gaagcagagc
    12481 accgcggtgg cggcgagccc cggggcggtc agcggcaggg tgatgcgcag gaaggcgccc
    12541 cagacgctgc agccgtcgat ccaggcggct tcctcgagcg acttcggcac ggtcgcgaag
    12601 aaggtctgca tcatccagat gacgagggcg aggttgaagg tgaggtaaat gaggacgagg
    12661 ccgaggatcg tgtcgctcag gccgagatag cgataggcga ggaagaacgg gatggtgaag
    12721 gcgatgggcg gcgccatgcg ggtcgcgagg atccacaagc ccacctgctc cttgcccctg
    12781 aactcccagc gcgccagcga ataggccgcc ggcgtgccga cgatcagcgc caggatggtc
    12841 gagatgccgc tcgagagcac ggagttcaaa aaggaggtcg ggaagttgcc gtgccagagg
    12901 gcggtgtagt gctcgacggt cgggctgaag agcagctcgt agccgaagat gtcgtcgttc
    12961 gggcggaacg acatctgcac catccagaag aacgggatca tgacgaaaag caggatggcg
    13021 acggccagcc agttgagcag ccggcggcgc cgggcctccg gcgagacctg cagcggccgc
    13081 ggcacggcgc ccgtgccgct cttcgcccgc tcgatggcgc tactcatcgc gctgccccgc
    13141 ctggtcgagc cgccggagca gccagctcag gaggaagacc ccggcgacca gcatcgtcgc
    13201 aatggcggac gcatagcccc agtaggagaa attgaacgcc tggacgaagg cgaagtagtt
    13261 ggtgacctcc gtcacctggc cggggccgcc attggtcagc acgtagatca agggaaaggc
    13321 cttgacgctg tcgatcagcc ggaagagggc ggcgacgatg atcgccggct ggatgtaggg
    13381 cagcaccacg taccagaagg actggtagct cgtggcgccg tcgatcttgg cggcctcgag
    13441 cggctcgtcc ggcaccatct gcaaggatgc caggatgatc agcatggtga aggggaacca
    13501 ttcccaggtg tcggcgacgg cgatcgccca gagtgcggtg tcggcctggg tgatcagcga
    13561 gcggaccgga aagcccacga actccagcag ccggtgcatc gggctgacgt cgggcgagta
    13621 gatgatcttc cagatcagcg ccacgacgat cggcggcagc accatcggga tgaggaaggc
    13681 ggtgcggatc gcctcgagca gcttggaccg cgcattcagc agcagcgcga ggccgagccc
    13741 gatcagcact tgcgccacga ccgtggcgag cgagagcttg acctggaccc agaccgagtt
    13801 gcggaagcgg tagtcggcga tggcttcccg gtagttgcgc aaggggtcgg agaaatcgag
    13861 cccggtctgc accgggttgg tcaggttcaa cggcgtgagg ctggtcacca ccagatagac
    13921 cgccggggcg acggtgatca ccgcgagaac ggcgagcgcc gggaacaggc cccagaagaa
    13981 cgcacgccgt cgctgccggt cccagctcgt gatcgccgcc gaggccatgg cccctcccgt
    14041 tgtcgaaccc ggccgccgcc ccggctggcg gagcggcggc tttcctgctt gcgcctggac
    14101 tagaggtcga cgccggcccg gcggatgtcg gcgatggcgt tctcctgcgc ctggcgccat
    14161 gcctcctcgg gcgtcgcctg gccgctggcg atcatctcga tcgccttgtt gatctgggcg
    14221 ttggcctgcg gatagacatg gaccgtgcgg taggccatgt ggccctgctc ggcgaggccg
    14281 atggtccgca ggtagatgtc gccgacatca tagccgttga ccatgttgac ctccttgaac
    14341 ttcgggctct cgatgaccga gcgccgggtg accgccccgt agcccttctc ctcgagcatc
    14401 cgcatcatca ggtccttgcc gaccgcccac ttgatgaatt cccagctcgc atccttgttc
    14461 ctggcaccgg tcgggatgcc ccagccgtgc acggcgaggc cggggaattt gcccttgggg
    14521 ccctcgggat acatggcgaa ggcgcaggtc tccttcgtct tgctgtcggg cgcacccatc
    14581 tgcatcagga agggctggtt gaagaggcag tagttggcgc ggccctgctt gagcacgttg
    14641 accgtctggt cataggtctg ggccagccag ccgtcgggcc cgtaattgtt ctgcaggtcg
    14701 acataccatc tcgaggcctc gtgcacctcg ggcgtgtcga gtgccggcat gatgttgtgc
    14761 ggcgggtcga cgaagacgtt gccgccgaag gcctgcatga tcggcgggaa gatccagccg
    14821 tggtgattct cggcggtgaa ggcggagacc cccttcacct tgtggatctc gggcaccatg
    14881 gccatgagct cgtcgaagct cttcggcatg ccgaacccgg cttcctccat gatgtcgaag
    14941 cgcgaggcgc cggcatgcat ggcgtcggcc acccaggaga agccgtaggt gcggccctcg
    15001 gcgtcccgca tcggcagctg cgtgccgttg aggaagtcgg cggcgtcgaa ctcgtccggc
    15061 gtcttgttcg ggtcgttgta gaagtcgtcg agcggcgtga accagccggc gccgatccag
    15121 cgcgacgtgt agatgaaggt gatgttggcg acgtcgtagg cggagccgcc ggtcgagagc
    15181 tcgaggtcga cctgctggtt gtagaccgcg aagccgggcg tgtcgtagtt gacctcgatg
    15241 ccggtgagct cggtgaactc cggcaggtat tcctgcagcc acttggcgta gggcgccgac
    15301 cacgacgaga tgttcaaggt ggtgcccgcg aagggcttgg ccccgccttg cgccggcgcc
    15361 ttgccgggca ggatcagggt ggagccgagg gccgcggtgc tgccgagcag cgagcgcctg
    15421 ctcatccgcc agatcgtcga catttagcct ctcccgcttg atggggcacc gggcatggac
    15481 cctttggtgc cgggtcttcg ttggatgccg ccgagcaggc gagccgcctg cctgctcgtt
    15541 atcctcgatc ttgacttcgc gcgggcatta gagcaagcac gaacaggtat ccattcaata
    15601 aaattcggta atcgctcacc catgcgggac ggaccgattg gtcggtcgga ccgtgggtgt
    15661 cgacagcctt cagggagacg tctcttgacc accaaccggt cgctcgccgg caaggtcgtg
    15721 ctggtcaccg gcgccagcag cggcatcggc gccgccattg ccgtcggctg cgccgcggcg
    15781 ggcgccaccc tcgtgctcgc cgcccggcgc gccgccgagc tgaagaccgt ggcggccgag
    15841 gcacgccgcc acgacgtgga ggtgctggcg gtcccgaccg acgtcaccga cccgcaagcg
    15901 gtcgacgccc tgatgcgggc caccctggag cggcacggcg ggctcgacat cgtcatgggc
    15961 atcgccggcg gcaataccgc ccgcggcgcc gtggccgaga tcgacgcggc cgagtggatc
    16021 gcctgcctca acctcaacgt caccggcggc ttcctcaccg ccagggccgc cgtgcccctg
    16081 ctcgaggcca agggcggcgg gcgcatcgtc ctgatgggct ccggcatggg ccatcgcggc
    16141 aatcccggcc acagcgccta ttgcgccggc aaggcggcgc aatggatgct ggtccgctgc
    16201 ctcgccgagg agctgaagga gaagaacatc tcggtcaacg agctgatccc ggggccggtg
    16261 atcacgccgg ccaccaccgc cgagatggcc gagggcggcc cgacctccgg cttctccagc
    16321 ccgggcgaat ggcgaaagca gcccgaggac gtggtgccca tggcgctctt cctcgccacc
    16381 tgcccggagc ccggccccac cgcccagagc ttcagcctga tgcggcggca gggctagccg
    16441 gcgatgggcg agaccatcgg ctatatcggg ctcggcgtga tgggcctgcc cttcgcccgc
    16501 cacctgaagg cggccgggca cgacgtggtc gtctacgatc gcgacgcggc ggcaagggcc
    16561 agggcggccg aggcgggcct gcgcgtcgca gccggcatcg ccgaggcggc acgcgacgcc
    16621 ggcatcgtct tcacctgcct gcccaacccg gcggcggtgc aggaggtcca tgccgagatc
    16681 ggccggcccg ggcagctcgc catcgacaac tcgacggtcg gtcccgacct cgcccgccga
    16741 ctccacgtcg agctcgcggc caggggcgcc gcctatgtcg aatgcccgat gctgggtggc
    16801 gtcggcgagg ccgaggccgg ccagctcttc ctcatcgtct cgggcgatgc ggccgccgtc
    16861 gagcgggcgc tgccgctggc gctcctggcg gcgcgcgacc accgggtggt cggcgggccg
    16921 ggggcggcga gcctcttcaa gaccgtgcag aacggcctcg gcctcgtgca gatgaccgcc
    16981 atcgccgagg cgctggcgct ggtggcggca ggcggcggcg atctcgaccg gttcatcgac
    17041 gtggtcggcg ccggcggcgg catggcggca accccgctct tccgcacgaa ggcgccgctg
    17101 atgcgcgacg ccggggcgaa ggcggtcggg cagttctaca tcggcgccaa ggatgccggc
    17161 ctcgccgcgg ccctcgccca ggcgcagggc ctcgacctgc cgctcttcgc cgccagcgac
    17221 agcgcttggc gcgaaggcat ggcggccggc ctcgccacgg ccgatgtcag cgccatcgca
    17281 cgggtcatcg aggcccgcac aaaaacgaaa atcgccggga gctgaatcgt tgcgcgaacg
    17341 cgagatcgag atagacgggc cggacggccg gctgcccgtc ttcgtcaccc ggccggagga
    17401 gggcgggccg catccgaccg tcatcctcta catggatgcg ctcggcattc gcgaggagct
    17461 gagggacatg gcccggcgga tcggcaccgt cggctactcg gtgatgctgc cgaacctctt
    17521 ctggcgcgag ggcggggcga gcttcgatcc ggccctcctg cccacgcacg gccccgaccc
    17581 cgagatgatg cgcctcaacc acgccaccac caatgcgatg gtgcaggccg acacggcgct
    17641 gctgctcgac catgccgccg cagacagtgc tgcgcgcgat cccgtgggcg tcatcggcta
    17701 ctgcatgggc gggcggcacg cgatgggcgc cgccggcacc ttccccgatc gcgtcgccgc
    17761 catggtctcg ctgcatggcg gcttccaggt gaccgaccgg ccggactccg cccatctcct
    17821 gatcccgagg atgcaggccg agtgctattt cggcttcgcc gacggcgatc ccctctcgcc
    17881 tgacgagcac cagcgggtca tggaagcggc ctgcgaggcg aacggcgtcg aggcccgctt
    17941 cgaggtctac gaaggggccc accacggctt caccttcccg acccgcttct gctaccacaa
    18001 gccgtccgcc gagcgcgcct gggagcgcgt cttcgcgctc ttccggcgcc ggctcggcta
    18061 gtcgccttgg cccttcagcc gcacctcgac gccggccttc gcctcgttga ccctgagcat
    18121 cgaggtgaag tcgtcgtcgc cgtagccggc ggcgatcgtc cggctcaggg tctcgtagac
    18181 gccatggccg accggcgcgt cgagcccggc cgccgtggcg aggccgaggc ccaggcggat
    18241 gtccttgtgc gacagcgtgg tcttgaagcc cgggctgaaa tcgccgtgga aggcgcgggc
    18301 cggcagcatc ttcgtcatga tgccgctcgt ggcgaaggtg gcgcccacca cctccaggat
    18361 ggtctcgagc tcgaggcccg acttgacccc catggtgagc ccctcgctca ggagcgccag
    18421 gatgccgccc gagatgtagt tgttgaccag cttgacggca tggccgttgc ccaaggggcc
    18481 gcaatgggtg atgtcggtgc ccatgcactt cagcaccggc atggcttcct ccacgtccgc
    18541 cgcctcgccg ccgaccatga tgctcagcgt gcccgcatag gcgttgtcga cggtccggcc
    18601 gaccggggca tcgaccatgc ggatgtggcg cgccgccatg gccgcgccca ccttgcgcgt
    18661 ggtcgcggga tcgaccgtgc tcatgtcgat gtagagggcg cccggcgcca ggccctcgat
    18721 gatgccgccc gcaccgaggg ccgccttctc cacgtcgggc gcgtccggca ccatggtgat
    18781 gacgatgtcg ctcgcctcgg ccaccgccgc cggcgagccc ttgcccgtcg cacccgcggc
    18841 gatgaggtcg gccacggccg actggtcgag atcgaagacg gcgagctcgt agccgccctt
    18901 cctgatgttc agcgccatgg gcttgccgat cgtgccgatg ccgacgaacc cgattcgctt
    18961 ggccatgagc gtctccactg tcttgttggt tgccggcggc ccggtcggcc gcaaagagcc
    19021 gtgttcgcgt ttccgcgtca aggggaggtc tgagaagatg cgctgtgtgt tcctgctgtt
    19081 cgattccctc aatcgccatt tgctcggccc ctacggcggc cagcggatcg agacgccgaa
    19141 tttcgaccgc ctggcgaagc ggtcgatcac tttcgacaac cactatgtgg gctcgatgcc
    19201 ctgcatgccg gcgaggcgcg acatgcagac cggccggctc tccttcctgc accgctcctg
    19261 ggggccgctc gagcccttcg acaacagctg gccgctgctg ctcgccgaga agggcgtcta
    19321 cagccacctg atcaccgacc acgcccacta cttcgaggac ggcggtgcga cctaccacac
    19381 ccgctacgac accttcgagt tcatccgcgg ccaggaaggt gacccctgga aggcgatggt
    19441 ccagccgccc tgggagcgcc tgcgcgagaa gtatcacgag cggcagtacg gcgagggcaa
    19501 ccgcacctac ttccgcaaca acatggtcaa ccgcgagtac atccgctccg aggccgactt
    19561 cccctcggtg cagtgcttcg ccgccgggct cgagttcctc gagaccaaca agggcgcgga
    19621 caactggctc ctgcagctcg agaccttcga cccgcacgag ccgttcaccg cacccgagcg
    19681 cttcaaggac gcctacagca ccggctggaa cggcccgatc cgcgactggc cgcgctacgc
    19741 ccgggtcgac gagctgccgg aggaatgcga ggagctcagg gccaactact acgcgctcgt
    19801 ggcgctctgc gaccacctgc tcggcacggt gctcgacttc ttcgacgcca acgacatgtg
    19861 gaaggatacc gtcctcatca tgacgacgga tcacggctat ctgctcggcg agcacgattt
    19921 ctgggcgaag aaccggatga ccatgtacga ggaggtggcc cacatccccc tcttcgtcca
    19981 ccaccccgac catgccgaca aggccggcca gcggcgcacg gcgctgaccc agaacatgga
    20041 catggccgcc accttcgtcg agctcttcgg cgccgagcgg ccggccgaga tgcaggccga
    20101 atccctcctg ccgcacctcg ccggcgacct cgagggcagg gaagccgtgc tcttcggcta
    20161 tttcggcggt gccgtgaacc tcgcggacgg gcactacacc taccatcgct acccgcccga
    20221 tctggcgacc caggagatct accagtacac gctgatgccg acccacatct ggacgcgctt
    20281 ctcggtcgag gagctcaggc ccgccacgct gagcgagccc ttggccttca ccaagggtgt
    20341 cccggtcctc aaggtgccga tgaccgagac gacgccgatc tacaacaatt acggcccggg
    20401 ctgcctgctc gaggacgaga cccggctcta cgacatcgtg gccgacccgg gccaggagcg
    20461 gccgctcgtc gagccggagc tcgaggcgcg gctcgccggc atcatggccc ggctgatggc
    20521 cggcaacgat gccccgcccg aggccttcgc ccggctgcgg ctcgagccgg cacccgcagg
    20581 ttgacccctt gggcagcgag acctagcgtg gcgacaacga atcagggagg gacaggatca
    20641 tgaagagctt ccgcaatgcc attctcacgc cgacccgccg cgccacgctg ggtctcggcg
    20701 ccggggcagc ggcgacgctg atcggcgccc cctacgtgct ccgccgggcg cgggcgcagg
    20761 gcgatttcga ctggcagcgg ttcgcgggcg agcacctcga ggtcttcctc tcgaagaacc
    20821 cgcgctccgc tttgctccag gagcacgagg ccgagttcgt cgagctgacc ggcatcaccg
    20881 tcggctcgga gcaggtgccg gaacagcagc atcgccaaaa gcaggtgatc gagttcacct
    20941 cgggcgccac cagcttcgac gtgaccatga ccgcctacca cgtccagaag cggctcttcg
    21001 ccaagggcgg ctggctcgag gatctccggc ccttcctcga caatcccgag ctgacgccgc
    21061 ccgactacga cttcgccgac ttctccgcgg gcgcggtcgc ctacgccacc caggcggacg
    21121 gccggatgga cacggtgccc ttcaacatcg acccctggat cgtctactgg aaccaggatc
    21181 tcttcgccga ggccggcatc gcgtatcccg acaccttcga cggcatgctc gaggcggcca
    21241 aagcgatcca cgcgccggac aagggcgtct acggcgtcgt cagccgcggc ctgaagaacg
    21301 ccaacacccc cgtctggacc ggcctcatgc tgggctggga catggaggcg gtcgacgccg
    21361 agggcaacct cacgacgacc tcccccgagg ccatcgccgc ggccgagctc tacaagacgc
    21421 tcaacgccga ctacgcgcct gaaggtgtgg tcggcttcaa ctggaacgag tgccagacga
    21481 gcttcatgca gggcaccgtc ggcatgtgga tcgacggcct cggcttcgcc aagccgctcg
    21541 aggacagcgc cacctcgaag gtcgcgggca aggtcggcta cggcatcttc ccggcggggc
    21601 cgacggcccg ccattcgccg ctcttcggct cgggcctcgg cgtctccgcc ttcagcccga
    21661 ggaaggaggc ggccttcctc tattgcgcct gggcgaccaa caaggtgaac cagatccgca
    21721 tgctgcagtc cggcgccggc gcaccctgcc gcaactcggc catcttcgac cccgaggtgc
    21781 tggcgagcct cgccttcccc gagcagtggg ccgagacgct ggtcgagtcg gcgcgcatcg
    21841 cccggcccgg cctgccggtc atcatcccgg tctccgagtt ccgcgacatc tacggcgtgg
    21901 cgctgtccaa catgatctcc ggcgccgacc cggccgacga gctcgcccgg gcgaccgagc
    21961 agttcaggcc ggtcctcgag aagagcgagc gcgcttgagg cacgggggcg gcgggaaggg
    22021 atgagcgtcg aggcgccggc cgggattgcc ctcgggagat cgggcaatcc cgcggtgcgg
    22081 cgctggcagt atctcctctt cgccgccccg gcgctcgtcg ccgtcttcct ggtgatcgtc
    22141 ttcccctggg cgttcaccgt cttcatgtcg atgcacgact ggggcgtggt cggcagccgc
    22201 acctacgtcg gcgccgacaa cttcctgcgc ctgctctccg acagccgctt catcgaatcg
    22261 atcggccaca ccctctactt caccgccctg gcgatcgtgc tgccggtcgt cctcggcgtc
    22321 ttcgccgccg tcgtcttcca ccgcagcttc ccgatgcgcg gcgtgctccg cgccatcttc
    22381 atcatgccga tgatggcgac acccgtggcc atcgccctcg tgtggaccat gatgttccac
    22441 ccgcagctcg gcgtgctcaa ctacctcctc acctcggtcg gcctgccacc ctccctttgg
    22501 gtctactcgc cgaccacggt catcccggcc ctcgtcctgg tcgaggtctg gcactggacg
    22561 ccgctcgtca tgctcatcgt cctcggcggg ctcgcctcgc tgccgaccga gccttacgaa
    22621 tcggcgctga tcgacggcgc ctcggcctgg cagatgttcc gccacatcac tttgccgctg
    22681 gtcatgccct tcatcatggt ggcggtgatc atccgcgcca tcgacgcgct caagaccttc
    22741 gacaccatct tcgtcatcac ccagggcggg ccgggcacgg ccagcgagac gatcaacatc
    22801 ttcctctatc tccaggcctt cgccttctac gatatcggct acgcctcggc ggtggtcgtc
    22861 gtcttcttcg tcatcatcgt cgtcttctcg ctgatcctgc tctgggcnnn nnnnnnnnnn
    22921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnntgg tcgtcgtctt cttcgtcatc atcgtcgtct
    23041 tctcgctgat cctgctctgg gcacggcagc gcacgctctg ggtggtcgac aattgatcgg
    23101 acgcatgaac gccacgggcc cgctggccca ggtcaagggc gccttctcgg tctcggcgaa
    23161 gcgcggcttc gaggtgcttt tcggcaagct cgccttcgcc ttcgctgtcc tcgtcatcgt
    23221 ctcgccggcg atcctcgtct tcctctggat gctctcgctg tcgctgaaga cggaggtcga
    23281 gaacacggcc ttcccgccgg tcttcatccc cggcaccctg acgctgcaga acttctacgc
    23341 ggtcttcgag agcagcccct ttgccctcta cacgctcaac agcatcatcg tctcgggcac
    23401 cgccaccggc ctcgccctcc tggtcggcgt ccccgccggc tacggcatcg ccaaggcgcg
    23461 ggccaacggc atcgccgtcc tcatcctgat cgcccgcatg acgccgggcc tctcctacct
    23521 gatcccgctc ttcatcatgt tccgcttcct gggcctgacc aactcgctca cccccatcat
    23581 catcacccac ctcgtcatca ccgtgccgat cgtggtgtgg atcatgatcg gcttcttcga
    23641 gaacctgccg cgcgagctcg aggaatcggc gctgatcgac ggcgcttccc tgtggcaggc
    23701 cttctggcgg atcgccctgc cgctctcccg cccgggcatc gtcgtggccg ccatcctcgc
    23761 cttcatcttc tcctggaaca acttcatctt cggcgtggtg ctggccggcc gcgagacccg
    23821 caccctgccc gtcgccgtct acaacgtgct gaccttcgag cagatctcct ggggcccgct
    23881 cgccgccgcc gccctcgtcg tcaccctgcc ggtcctcgtg ctgacggtcg tcatccagaa
    23941 gcagatcgtg ggcggcctca cctcgggtgc cgtgaagggt ggctgagcac acggtgaacg
    24001 gcctgaagaa ggaactgcac acttgacgct gaacgccccg tccggcgcgc ccgaccacga
    24061 cgccatcatc gtcggcgccg ggttcgccgg cctctacatg ctgcacaagc tgcgcggcca
    24121 gggcctgcgc gcgcacgtct tcgaggccgc aggcggggtc ggcggcacct ggtggtggaa
    24181 ccgctacccg ggtgcccgct gcgacgtccc gagcgtcgag tactgctact ccttcgacga
    24241 gggcctgcag caggactgga gctggagcga gcgctacgcc acccaggccg aaatcctggc
    24301 ctatgccgag cacgtcgccg accgcttcga tctgcgacgc gacatcacct tcgaggcccg
    24361 ggtcgagacc gccatcctcg atgaggcaga ggcgatctgg gaggtcgaga ccacgaaggg
    24421 ccgggccagc gcccgcttcc tgatcctcgc caccggcaat ctctcggtgc ccaattggcc
    24481 ggccattccg ggcctcgacc ggttccgggg ccgcgtgctg cacaccggcg cctggccgca
    24541 cgagccggtc gacctcgagg gccaaagggt ggcggtgatc ggcaccggct cctcggccgt
    24601 ccagtcgatc ccggcgatcg cccgcgaagc cgcccgggtc cacgtcttcc agcgcacccc
    24661 ggcttacgcc gtgccggcgc acaaccaccc gctcaccgaa gagcagcggg cggcgatcaa
    24721 ggccgactac ccggccttgc gtgccgccgc cctgacccgc cgcaactaca tcaacttccc
    24781 gcagaaccag caaaaggcga tgtcggtcga tgccgccacc cgccgggccg agtacgaggc
    24841 gcgctgggcc acgggcgggc tcagcttcaa cggcgccttc gtcgaccagc tcatcgaccg
    24901 ccgcgccaat gacgaggcgg cggcgttcgt ccgggaaaag atccaaaaga tcgtcctgga
    24961 ccccgagacc gcggccaggc
//
LOCUS       DAOTFI010000030        24949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2327_length_24949_cov_7.731823, whole genome shotgun sequence.
ACCESSION   DAOTFI010000030 DAOTFI010000000
VERSION     DAOTFI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 24949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24949
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_24949_cov_7.731823"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1229
                     /locus_tag="P5558_04570"
     CDS             228..1229
                     /locus_tag="P5558_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HRY23617.1"
                     /translation="MAQLNVGLILTRRFTLCALGNFVDVLRLAADEGDRSRPILCAWK
                     VLSPTMEPIASSSGIVVQPDEPLGDPARFDYLVVVGGLVDEVERLHTDTVRFLERAAA
                     AGIPLVGVCTGTYVLHRAGLMDGYRACVSWFHHDDFLERFDGLKPVSDQIFVVDRDRL
                     TCSGGVAAAHLAAFIVERHLGRARAAKSLHIMIIDEAAAEEPQPGMPLALATDDALVR
                     RALLVMQQSIDSPLSIGRVVVQLAVSRRKLERHFRQALGMTPLEADRLIRLEQAKHLL
                     VTTARSATRIANETGFCDLPHMIKVFRAAEGITPDVFRRSGRGSTAVSALPPTVGSS"
     gene            complement(1186..2160)
                     /locus_tag="P5558_04575"
     CDS             complement(1186..2160)
                     /locus_tag="P5558_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HRY23618.1"
                     /translation="MSVGFILARRFTLCAFANFVDVLRLAADEGDRSRPILCEWSILS
                     ESMDPIASSCGVAVQPGERLGDPARFDYIVVVGGLLEEIPNIGPTYTRFLARAAAAGV
                     PLVGVCTGAFLLHRAGLMDGYRACVSWFCHADFLEQFDGLEPISDQIFVVDRDRLTCA
                     GGASSAHLAAYLVEKHVGRAQASKSLHIMIIDDALRPEKPQPGIPLDFRTEDPMVRRA
                     LLLMQQRIDAPLSVAELARQMGNSKRQLERHFRTALDTSPQAAFLDIRLSFARHLLEV
                     TDKSISAIAVECGFCDSSHLSRLFRRRYDSTPFEIRKIRPLVEAPTPQ"
     gene            2399..2644
                     /locus_tag="P5558_04580"
                     /pseudo
     CDS             2399..2644
                     /locus_tag="P5558_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889670.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
     gene            2647..3072
                     /locus_tag="P5558_04585"
                     /pseudo
     CDS             2647..3072
                     /locus_tag="P5558_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
     gene            3200..5761
                     /locus_tag="P5558_04590"
     CDS             3200..5761
                     /locus_tag="P5558_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY23619.1"
                     /translation="MSAFPERARVVIVGLGGIVGASVAHHLVERGWDDIVGIDKSGIP
                     TDIGSTAHASDFCYATSHDFLSCWTTLYSVDFFEKLGHYARIGGIEVARVGDDERMNE
                     IRRKVDSGKAFGTRARLMEPAEIKEKFPLIEEDLVQGGLWDPDAGLVIPRSQTVAGKL
                     VDQAEAAGKLKAFANTSAKSLIIEDGRIKGVITTRGTIMADYVVVCAGLWGRLIAEMA
                     GEDLPVMPVDHPLTFFGPFDKFAGTGKEIGYPLMRDQGNAAYMRDTGDPKTAEGGQIE
                     WGYYEETNPRLVHPRDILEKEQARLSPSQRDLDMEQVIEPLERAMELTPILGELGFNE
                     GHSFNGLLQVTTDGGPSMGESQKVRGLWYAVGIWVKDGPGMGKLIADWMTDGRTAIDH
                     ASIDYARFNPFALTERFIEERCTETARKIYNPPVHPREPFDGGRGIRRSPFWEREKEL
                     GGYFMELGGWERAHGYAANEHLLEKYGNQVPVRENEWDSRHFWRVSNAEQLAMSEDCG
                     IINLSHFHMTDIEGPDHVALMEWLCAAKVGGDGNIGKGIYTHFLDDEGMVRADFTIFR
                     MPGRIRMVNGADAGPRDFHYMRRVAEDKGFDVTITDVAEKYVTIGIWGPNARVNLQKV
                     VEDPEGLSKENFPFAAIRPVRIAGRDVTAFRISYVGEQGWELHMAYEDGLAVWDALRS
                     TGVTAVGVETYANSRRLEKSLRLQNADLRTEYNLFEADLARPKVKEADFRGKAKHLEY
                     RAREHQPAMLCTLVITDNVDARGVARYPVGILPVMDPETGETLVDELGRRSYTTSIAF
                     GPSIGRNIALAYLPWAYCQEGRKLSVRYFGETYPVEVVGIGYKPLYDPENAKPRS"
     gene            5857..7101
                     /locus_tag="P5558_04595"
     CDS             5857..7101
                     /locus_tag="P5558_04595"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRY23620.1"
                     /translation="MIVDDVAKLSLLEDRKPDHALPRPFYTDAEIFDLDLRRVWYRDW
                     LFAIPSCEIPQTGNFVTMQVGEYAIVIVRGADGVIRAFHNSCRHRGSRVCTVLKGSAP
                     KLVCPYHQWTYELDGRLLYARDMGPDFKAADHGLKPVACVDAGGVVYVCLADQPPAID
                     GFVNSARRYFAPHRLNEAKIAYESTIVEKGNWKLVIENNRECYHCAGAHPELCRTYPD
                     RPGFTSMDESGAIEPAVTAHWEKCEAAGLPSKFVIAPDHQWRFARIPLLGPGESYTMD
                     GKAAVPTKRLSNSPFADAGSLLNFHYPNTWNHFLADHSIVFRVLPISPTETEVTTKWL
                     VHKDAVEGVDYKLENLTRVWIATNDEDRQVVEENQRGINSPAFVPGPYSTIQEDGVIQ
                     FVDWYCRTIGNRVGGGGRLAAE"
     gene            7182..8192
                     /locus_tag="P5558_04600"
     CDS             7182..8192
                     /locus_tag="P5558_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid-cluster NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23621.1"
                     /translation="MVPDGPGTVSFTFRSPSGAWFDYRPGQFLTLELPVSGGPIHRTY
                     TISSSPSRPLSITLTAKASASSVGTRWMLDNLAPGTRIKAIGPGGIFVPDVPADRKLL
                     LLSAGSGVTPMMSMTTYRYDLGGKPDICFVQCAKRPSELVFRRQLEAMARRVPTITLH
                     YVVTQDDPYDAWTGYRGTFNQLMLGLMAPDYLEREVYCCGPEPFMQTVREMLIGFGFD
                     MDHYHQEAFTGPPVLTPADAPERDDVVPDAEAASEVIFTLSGRSVPCTETDTILAVSR
                     TAGLNIPSGCNFGVCGTCKTRKLSGEVHMVHNGGISEGDIAAGYILACCSLPMGRVEI
                     EA"
     gene            complement(8383..8904)
                     /locus_tag="P5558_04605"
     CDS             complement(8383..8904)
                     /locus_tag="P5558_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY23622.1"
                     /translation="MTEEAERPPGAVIETCATDEHRIGLKPVLRRVQAPRGERPVARG
                     HHRFEWLYVTAFVSPATGESHWYLATGVDRQLFEETLALFAREAGAGRDRIIVLDGAG
                     LHTLPLAVPESVRLVYLPAYTPELQPAETLSGPYRRADRQPALQDHCRSRRHHRRQVR
                     LPCQRPRSRPRPS"
     gene            complement(8922..9398)
                     /locus_tag="P5558_04610"
     CDS             complement(8922..9398)
                     /locus_tag="P5558_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRY23623.1"
                     /translation="MAHVAGHLSVDELQAGYRGSDDATPARHCHVIWLLVQGRTCADV
                     AGLASFALRRVEQLLERYNAFGPGNIGDRRRGNGAAPTVLTPEMMAAVRERARRQPDD
                     GGVWLARNVAAFMAAELGRRVAEQRGWEPLCAIGWTIQSPRPRHVESATPEEQAAF"
     gene            9618..9989
                     /locus_tag="P5558_04615"
     CDS             9618..9989
                     /locus_tag="P5558_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23624.1"
                     /translation="MSETSNDPALAAGMCPADGPTGIINTDYTIGRDNIEGSLGPLGF
                     DMHNPVFAISALSVIAFVAFTLMRSVSWYHGAGPPNVSDRKSRSRPGFGTWSGEVLQR
                     RAGPGLLLLEPGHDRRKSPEG"
     gene            complement(10025..10324)
                     /locus_tag="P5558_04620"
     CDS             complement(10025..10324)
                     /locus_tag="P5558_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRY23625.1"
                     /translation="MARLVTDMLADREDARLTLYLRDAGKLHAVPANARVVEADVLDA
                     PRLAAALEGQDVVYANLSGDVDEPATAIVSAMEAAGIRKLVFVTSLGIYDDPNRL"
     gene            complement(10408..11352)
                     /locus_tag="P5558_04625"
     CDS             complement(10408..11352)
                     /locus_tag="P5558_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY23626.1"
                     /translation="MAWHIAADIRFPPGFDERRKYPAIVSVHPFGSCKEQTSGNIYGR
                     ALAKEGFVVIAYDASFQGESGGTPRWIEDPTQRVEDVSRVIDYAITLPYVDVERIGVL
                     GVCGGGGYAINATLTEKRIKAVVSITGVNLGRLFREGFSSYDPLGALDAMAAQRTSEA
                     RDGELQVNELLPASPEAATNAGLTERDVFEATDYYKTPRGRKPGGSTRMLFSHAQKTL
                     AWDAFAFAETLLTQPVMVVIGQKVGAFGAFRDGMEVYGRANASRDRQLVSLEGWSHYD
                     LYDKPDPVAKALERLVPFFRQHLGETVATDAREPVAAG"
     gene            complement(11446..12384)
                     /locus_tag="P5558_04630"
     CDS             complement(11446..12384)
                     /locus_tag="P5558_04630"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY23627.1"
                     /translation="MGKISFPNSNNQTIAMSAVINFPEGFDESRSYPAVVVSHPGGGV
                     KEQTAGLYARKLAEEGFVTIAYDASHQGESGGEPRQLENPHIRTEDVSAVIDYLTTLP
                     YVDKERIGAMGICAGAGYTANAAINDPRIKAVGTVSAVNIGSMFRNGWENNVKDADAS
                     PYVKAGSDARTSDASGAETATIPLAPLKEEDAPNEELRQAWEYYHTPRCEYPTAPGYA
                     TARSLTQIIPYDAYNKAEAFLTQPLQVVAGSVAGSKWMSDDLFSRAASADKHFHVVEG
                     SNHMDLYDRDQFVNEAVGVLAPFFQRILAPELTPAE"
     gene            complement(12481..13392)
                     /locus_tag="P5558_04635"
     CDS             complement(12481..13392)
                     /locus_tag="P5558_04635"
                     /inference="COORDINATES: protein motif:HMM:NF018431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRY23628.1"
                     /translation="MDGNGIAVRTEATTAPLARAIALHLPRAGDVATAVPELSLHRRD
                     APTEPVDCTYGLGLIVTTQGAKQVILGDRVVAYRPGQSMLTTMELPITSHVMEASLRA
                     PYLGLLLKLDIRAIAVAAGEIDAVIAVPGQTPLPPASIETLDPRLLDALERLVALIDD
                     PLRPHLAGLVRQEIVLRLLAGPHGGLLRRLVAQQAPREQIGVVIAWLKANFTQMVRIE
                     DLAARAHMSPSTFRQHFRAVAGMSPLQYQKQLRLQEARQLMLNRSVTAGRAAVLVGYE
                     SDSQFSREYSRLFGAPPQRDVQRLRSN"
     gene            13497..14660
                     /locus_tag="P5558_04640"
     CDS             13497..14660
                     /locus_tag="P5558_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRY23629.1"
                     /translation="MASPAAFLSQDGTGAATGLGGPAAERGREDLMRGMVFRGDRKVE
                     LLDFDDPTPGQGEAVIEIKASGMCGSDLHFYRNNPAEVIRSLGFKDLASRGIDENTPI
                     IGGHEPCGVVAALGPGVDPKAFGVGDRVMVFHYEGCTHCDHCRTGWTQMCDHGAIVHG
                     VIAHGAHADYMKVPASCLVPMPEGLSFAGGAAVACGTGTAYGALTRLDISARDTLTVF
                     GLGPVGLSAAQLAAAMGIEVIGVDISADRVERAKDFGVAHAIDGSSTDPVEAIRSLTR
                     GKGVTCAMDCAGGEAPKQQAVRATAPWGRIALVAVGGNLNVDAMKDLIGKQRTVIGSY
                     TFSSVGMKGCAEFITDHGVEVDKLFTDQWRLDQAEEAYRSFDKQAGGKGVFLF"
     gene            14885..15898
                     /gene="dctP"
                     /locus_tag="P5558_04645"
     CDS             14885..15898
                     /gene="dctP"
                     /locus_tag="P5558_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY23630.1"
                     /translation="MFAKRVTAAILAASTLAGAASAQETATLKFAHVFPAEHYLWTHG
                     GKVFADAVAEATDGRITFQVFPAAQLGKDYVSLLKSNLAESALLVPSYTPESLSLTSV
                     SELPGLYSASCEATAKLWEIARPGGVLNADEYAPQGLHVLFVSTLPPFKVLTASQPIA
                     EIADLAGLKIRANGAAMDKTVRTLGATPIQVTAGELYDSVTRGTIDGGFFPFHSVPTY
                     HLEEVFGFGVEGAQLGSGSVVFAMSDRAWSKLGPDDQAALTEAAAAAQQNLCQWLDAE
                     EARVRDLLVAENGLQVTSLSPDQAVAWQEAVAAVATDWAADLDRLGRPGTAVLEAFRD
                     AAP"
     gene            15909..17375
                     /locus_tag="P5558_04650"
     CDS             15909..17375
                     /locus_tag="P5558_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRY23631.1"
                     /translation="MVWQGHYDRLFIGGDWIAPSTGARIAVTSPLTEEVIAEVPDAAR
                     TDVDRAVAAARAAFDTGPWPRMPLDERMAVLACFRDIIAERRDQVAQLVTDEMGCPIT
                     LSKGMQATGPKLLLDAFLELAPGYPWQDVRRSTTGTGLVLREPKGVVAAVVPWNAPIL
                     IAAIKLAPALLSGCTMILKPAPESPLSAYLLAEMLAEAGLPAGVFNVVPADREVSEYL
                     VLHEGVDKVSFTGSSAAGRRLGGICGERLRHLTLELGGKSAGIVLDDADLPSVMQAIR
                     ATSLRNSGQVCSNKTRIVVSRRRRDELLDALSAMMATMPVGDPNDPATEIGPMVSHRQ
                     RERVEGYIARGREESRLVLGGGRPAGLNRGWFVEPTIFADVDRNAVIAQEEIFGPVLA
                     VQTYEDEDDAVAIANDSTYGLNGAVHSADPERALRLARRIETGTVEINGAGVGFHSPI
                     GGVKMSGIGREAGPEGFDAYVDIKSIGLPAGLADKFAN"
     gene            complement(17442..18470)
                     /gene="dctP"
                     /locus_tag="P5558_04655"
     CDS             complement(17442..18470)
                     /gene="dctP"
                     /locus_tag="P5558_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY23632.1"
                     /translation="MSMNRLLAGAATCAVLCGTTIAAGAQDVTLRLSNTFPENHFLWQ
                     NGGKVFVDAVDEATSGAVTVEAFHAGQLGKDQLGLLDSGLAQMAVIAAPYAPEKLPLT
                     GVVELPGFISGACEGADRVWPLVREGGALDQHEYEPLGLRVLFIAVPPPYKVMTGSTA
                     VTGLADLAGLKLRTVGAAQGDTVVAVGATPIQVQASEFYDSVSRGTVDGAIYAYVGMP
                     PYSLGEVMHHAVDNVFAGSLAVLYAIDADTWAGLTPEIQTAMTEAAATAQRAVCEYQD
                     ADEAAIRERYVAEKGLTVVSFPESDTAAWNERMAGVAAAWAKRLDENGRPGTEILEAY
                     RTSGASGD"
     gene            complement(18510..20000)
                     /locus_tag="P5558_04660"
     CDS             complement(18510..20000)
                     /locus_tag="P5558_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY23633.1"
                     /translation="MTTMELPKVEVPAFVAGTKGLLIGGQWVAPRSGRTIDAINPATG
                     ETLCRIGAGDAADVDLAVAAARRAFEGSWSRWTPHERRKLILRVHDVLMDHFDELALI
                     ETLDMGAPLARTRPLREWTSQVLQFYASQTGAATVETPPNSLPGDFLTLKHKAPVGVV
                     GGVIPWNGPLIGQWWIFGPTLATGCTAVIKPAEDASLSVLRTAELMLEAGVPEGVINV
                     VTGYGREAGQALAEHMDVDRVAFTGSYLTAQSIVRASAGNMKRIQLELGGKSPDIVFA
                     DADLDKAVPGAAMGVFTNSGQVCVAGTRILVHRAIHDEFVERMQAFTRSIRVGNGLAP
                     DTQLGPLISQRQLDRVLGYLEVGAREGARLAAGGTRLGGDLASGFFVEPTVFAGVSND
                     MTIAREEIFGPVASIIPFGDMDEALRIANDTPFGLAGGVWTRNLSTAHKVSRAVRAGT
                     VWVNCYGVLDPSIGFGGHKMSGYGWKGAREHVDSFLYPKAVYMNLD"
     gene            complement(20772..21944)
                     /gene="bktB"
                     /locus_tag="P5558_04665"
                     /pseudo
     CDS             complement(20772..21944)
                     /gene="bktB"
                     /locus_tag="P5558_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
     gene            complement(22055..23698)
                     /locus_tag="P5558_04670"
     CDS             complement(22055..23698)
                     /locus_tag="P5558_04670"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HRY23634.1"
                     /translation="MTREAMDYDVVIVGAGPAGLAAAIRLKQLDPELSVVLLEKGSEV
                     GAHILSGAVLDPSGLDALLPDWREMDAPIHTEVTEDRFYLLGRSGKVRIPTWPMPPLM
                     SNHGNYVVSMANVCRWLAGIAEGLGVEIFPGMACSDLVYDGDRVAGVVAGEFGREADG
                     TPGPGYEPGMELRGKYVFLAEGVRGSLSKQAIARYGLSEGYEPQKFGLGMKEIWEVQP
                     EVHRPGTVVHTMGWPLGSNAGGGSFLYHFEDNQVLVGFVVHLNYRNPHLSPYMEFQRL
                     KHHPLIADVLRDGKRVAYGARAISEGGWQSIPKLAFPGGALLGCSAGLVNVPRIKGNH
                     NAMLSGIAAAEAAHAALAAGRQGDELEDYETAVREGEIGRDLKPVRNVKPMWSKWGLW
                     ASLAFGGLDMWTRSLTGLSLFGTLGHGKSDAEATGQAKDFAPIEYPKPDGVLSFDRLT
                     NVAFSFTNHEESQPCHLRLRDPSLPIAVNLPKYSEPAQRYCPAGVYEVLRDTDGSSPR
                     FQINFQNCVHCKTCDIKDPLQNIDWTTPQGGDGPNYPNM"
     gene            complement(23794..24723)
                     /locus_tag="P5558_04675"
     CDS             complement(23794..24723)
                     /locus_tag="P5558_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY23635.1"
                     /translation="MAVLILAKHDNKSLNDATAKTVTAAKAMGGDITVLVAGENVGAV
                     AEAAAKLDGVKKVIHVEGEAYGHRLAEPLTDLVVSMAGPYEHIVFPATTSGKNVAPRV
                     AALLDVMVVSDITGVVAADTFERPIYAGNGIQTVQSSDPKKLVTVRPASFGATGEQAP
                     APVEIGTAVSDPGLSSWVEDKVATSDRPELTSAKIVVSGGRGVGSEENFRVIEKLADK
                     LGAAVGASRAAVDSGFAPNDWQVGQTGKVVAPELYNAVGISGAIQHLAGMKDSKVIVA
                     INKDEEAPIFQVADYGLVGDLFQIVPELEEKLS"
     gene            complement(24723..>24949)
                     /locus_tag="P5558_04680"
     CDS             complement(24723..>24949)
                     /locus_tag="P5558_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010503777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRY23636.1"
                     /translation="TDLRLNEPRYASLPNIMKAKKKPLEEKTAADYGVDVAPRLKVVK
                     TVEPATRKAGVKVGSVDELVAKLKTEAGVI"
BASE COUNT         3863 a         8673 c         8328 g         4085 t
ORIGIN      
        1 cgccggcacc gacgatgacc acgtcgtagg ccgccttcgg ctcgcggtcg ggccattgtg
       61 gggtccagtt cttctggccg gtcagcgcgc ccttgatgac ctgggcgaag gagaattgcg
      121 tcatggcggg cccccctgga ttgccggacc tctatactgg attcgtcgcg ggctgacgtg
      181 tacccttgcg tcagtttcgc ggtagctagg cggcaggaga tcgtgggttg gcccagctca
      241 acgtcggcct catcctcacg cgccgcttca cgctctgcgc gctcggcaat ttcgtggacg
      301 tgttgcgcct cgccgccgac gagggcgacc gctcgcggcc catcctctgt gcctggaagg
      361 tgctatcacc gacgatggag ccgatcgctt cgagctcggg catcgtcgtc cagccggacg
      421 agcctctggg cgacccggcc cgcttcgact atctcgttgt cgtcggcggg ctcgtcgacg
      481 aggtcgagcg cctgcacacc gacaccgtgc gtttcctgga gcgcgctgcg gcggcaggca
      541 tcccgctggt gggtgtctgc accgggacat acgtgctgca ccgggccggg ctgatggacg
      601 gctatcgggc ctgcgtcagc tggttccacc atgacgactt cctcgagcgg ttcgacgggc
      661 tcaagccggt ctccgatcag atcttcgtcg tcgaccgcga ccggctcacc tgctccggtg
      721 gcgtcgcggc ggcgcatctc gccgccttca tcgtcgagcg ccatctcggg cgagcgcgcg
      781 cggcgaagag cctgcacatc atgatcatcg acgaggcggc tgcggaggag ccccagccgg
      841 gcatgccgct cgcgctcgcc accgacgacg cgctggtgcg gcgggcccta ctcgtcatgc
      901 agcagtctat cgactcgccg ctttcgatcg gccgcgtcgt cgtccagctg gcagtgagcc
      961 ggcgcaagct cgagcggcac ttccgccagg cgctcggcat gacgccgctg gaagccgatc
     1021 gtctgatccg gctcgagcag gcgaagcacc tgctcgtcac gaccgcgcgg tccgccaccc
     1081 ggatcgcgaa cgagactggc ttctgcgacc tgccgcacat gatcaaggtg tttcgcgccg
     1141 cggagggcat caccccggat gtctttcgcc gtagcggtcg cggttctact gcggtgtcgg
     1201 cgcttccacc aacggtcgga tcttcctgat ctcgaacggc gtcgagtcgt agcggcgccg
     1261 gaacaatcgg ctgagatgcg aggagtcgca gaagccgcac tcgacggcga tagcggagat
     1321 cgacttgtcg gtcacttcga gcagatgccg ggcgaatgac aggcggatgt cgaggaacgc
     1381 tgcctgcggc gacgtgtcga gggcggtccg gaagtgccgt tcgagctgac gcttgctgtt
     1441 gcccatctgg cgcgctagct cggcgacgga aaggggtgcg tcgatgcgct gctgcatgag
     1501 gagcagcgcc cgcctgacca tcgggtcctc ggtcctgaaa tccagaggaa ttccaggctg
     1561 cggcttttcc ggccgcaggg cgtcgtcgat gatcatgatg tgcaggctct tgctggcttg
     1621 cgcgcgcccg acatgtttct cgacgaggta ggcggcgagg tgcgcggagc tggcgccgcc
     1681 ggcgcaggtg agcctatccc ggtccaccac gaagatctgg tcggagatcg gctcgagccc
     1741 gtcgaattgt tccaggaagt ccgcgtggca gaaccagctg acacaggcac ggtatccgtc
     1801 catgagtccg gcgcggtgca ggaggaaggc tcccgtgcag acgccgacga gcgggacgcc
     1861 ggccgccgcg gcgcgcgcga gaaagcgggt gtaggtgggg ccgatgttgg ggatttcctc
     1921 gagcaggccc ccgaccacga cgatgtagtc gaacctggcc ggatccccca ggcgctcacc
     1981 cggctgcacg gcgacgccgc aactcgacgc gatcgggtcc atcgactcgg agaggatgga
     2041 ccactcgcag aggatcggcc ggctccgatc cccctcgtcc gcagctagtc tcaggacgtc
     2101 gacgaagttg gcgaaagcgc acagggtgaa gcgcctggcc aggatgaagc cgaccgacaa
     2161 gcgcggcccg ccggccctgg gtcttgcggc ccttgcggga aggggttgcg atccatgtcg
     2221 gctcgcgccg gcccccggct tcatccctcg gttgctcccc tgtcgtagct ctcatccgcg
     2281 attgtcgcaa acatcctgtc ggcttcaagc ggaatgtcgc attgctttcg cgcaagctcc
     2341 tgggtcccga ggacacggcg acacgggcag cgggcttccg accgaaaagg cgcggcacat
     2401 gagggtcctg gtccccgtca ggcgggtggt cgacttcaac gtcaagatcc gtgcgaaggc
     2461 ggatggctcg ggcgtcgatc tgtcgaacgt gaagatgtcg atgaacccgt tcgacgagat
     2521 tgcgctcgag gaggcgatcc ggctgaagga agccaggaag gcggatgagg tcgtcgctgt
     2581 cacgatcggt ccggcccagg cccaggcgac cctgcgcaca gcgctcgcca tgggagcgga
     2641 tcggcgctcg gctcgctggc gggcctcgac gccattggcg tcggccggtc gattctcgtc
     2701 ggcaagccga tggcgcagct cctgctggcg gagaattgca ctgtgaccgt cgcgcattcg
     2761 cggacgcgcg atcctgcgtc tacggtcggc gcggcggaca tcgtcgtcgc ggcggtcgga
     2821 caggcggaaa tgatcaaggg cgactggatc aagccgggcg caacggttat cgaagtcggc
     2881 acgagccgga tcgtccgcga cggaaagacg gcgctggtcg gggacgtgga gttcgcggcg
     2941 gcctcatgcc acgcgggcgc taaaacgccg gtacccggag gtgtgggtcc aatgacgatc
     3001 atgagtccgg ttcacaacac gctcgtggcg gcgcgccggc gcaggggcct tcgcgggccg
     3061 cagctttgtt agatgtcggt ggtgcgtgaa gctcaacggg tgtctccgac gcgcgctcga
     3121 ccggcgctga cggacatgcc gccgatcgcg acgctcgata gattgaccct gccaccccag
     3181 acagagaagg attgaagaga tgtcagcgtt tcccgaacgg gcgagggtgg tcatcgtcgg
     3241 gctcggcggt atcgtcggcg cgtccgtcgc gcaccacctt gtcgagcgcg gctgggacga
     3301 cattgtcggc atcgacaagt caggcatccc gaccgacatc ggctcgaccg cgcacgcctc
     3361 ggacttctgc tatgccacga gccacgactt cctcagctgc tggacgacgc tctactccgt
     3421 cgacttcttc gagaagttgg gtcactacgc ccggatcggc ggcatcgagg tcgcgcgggt
     3481 cggcgacgac gagcggatga acgagatccg gcgcaaggtc gactcgggga aagccttcgg
     3541 cacccgcgcg cggctgatgg agccggccga gatcaaggag aagttcccgc tgatcgagga
     3601 ggacctcgtc cagggcggtc tctgggaccc ggacgccggc ctcgtcatcc cgcgctcaca
     3661 gaccgtcgcc ggcaagctcg tcgaccaggc cgaggcggcg ggcaagctca aggccttcgc
     3721 caatacctcg gcaaagtcgc tgatcatcga ggacgggcgc atcaagggcg tcatcaccac
     3781 ccgcggcacg atcatggcgg actacgtcgt ggtctgcgcc ggtctctggg gccggctgat
     3841 cgccgaaatg gccggtgaag atctgccggt gatgccggtc gaccatcccc tgaccttctt
     3901 cggtccgttc gacaagttcg ccggcaccgg caaggagatc ggctatccgc tgatgcgcga
     3961 ccagggcaac gcggcctaca tgcgcgacac cggcgacccg aagaccgccg agggcggcca
     4021 gatcgagtgg ggatactacg aggagaccaa cccccgcctc gtgcatccgc gcgacattct
     4081 cgagaaggag caggcgcggc tctcgccgtc gcagcgcgac ctcgacatgg agcaggtgat
     4141 cgagcccctg gagcgggcga tggagctgac gccgatcctc ggcgagcttg gcttcaacga
     4201 gggccattcc ttcaacggcc tcctgcaggt gaccaccgac ggcggtccgt cgatgggcga
     4261 gagccagaag gtcagggggc tctggtacgc ggtcggcatc tgggtcaagg acggccccgg
     4321 catgggcaag ctcatcgccg actggatgac cgacgggcgc accgcgatcg accacgcgag
     4381 catcgactac gcgcgcttca acccgttcgc gctgaccgag cggttcatcg aggagcgctg
     4441 caccgagacc gcccgcaaga tctacaaccc gccggtgcat ccgcgcgagc ccttcgacgg
     4501 cgggcggggg atccggcgct cgccgttctg ggagcgggag aaggagctcg gcggctactt
     4561 catggagctt ggcggctggg agcgggcgca tggctacgcg gcgaacgagc acctgctgga
     4621 gaagtacggc aaccaggtgc cggtgcgcga gaacgagtgg gacagccggc acttctggcg
     4681 ggtgtcgaat gccgagcagc tcgccatgag cgaggattgc ggcatcatca acctgtcgca
     4741 cttccacatg accgacatcg aggggccgga ccatgtggcg ctgatggagt ggctgtgcgc
     4801 cgccaaggtc ggcggcgacg gcaacatcgg caagggcatc tacacccact tcctcgacga
     4861 cgaggggatg gtgcgcgccg acttcaccat cttccggatg cccggccgca tccgcatggt
     4921 gaatggcgcc gacgccgggc cgcgcgactt ccactacatg cggcgtgtcg ccgaggacaa
     4981 gggcttcgac gtgacgatca ccgacgtcgc agagaagtac gttacgatcg gcatctgggg
     5041 gccgaacgcg cgggtgaacc tgcagaaggt ggtcgaggac ccggaagggc tctcgaagga
     5101 gaacttcccg ttcgcggcga tcaggcccgt ccggatcgcc ggccgcgacg tcaccgcctt
     5161 ccgcatctca tacgtcggcg agcagggctg ggagctgcac atggcctacg aggacgggct
     5221 cgccgtctgg gacgcgctgc gctccaccgg cgtcaccgcg gtgggggtcg agacctacgc
     5281 caactcccgc cggctcgaga agagcctgcg tctgcagaac gccgacctgc gcacggagta
     5341 caacctcttc gaggccgacc tcgcgcggcc caaggtcaag gaggcggact tccgcggaaa
     5401 ggcgaagcac ctcgagtacc gtgcccgcga gcaccagccg gcgatgctct gcacgctggt
     5461 gatcaccgac aacgtcgatg cgaggggcgt tgcgcgttat ccggtcggca tcctgccggt
     5521 catggacccg gagaccggcg agacgctggt cgacgaactc gggcggcggt cctacaccac
     5581 ctcgatcgcc ttcgggccga gcatcggcag gaacatcgcg ctcgcttacc ttccctgggc
     5641 ctactgccag gagggccgga agctctcggt ccggtacttc ggagagacct acccggtcga
     5701 agtcgtgggc atcggctaca agccgctgta cgatccggag aatgccaagc ccaggagctg
     5761 agtctctctc agatgcccgg cggcgcctgc cggatgcatc ctgccaccac atgctcgcag
     5821 acggttgcgg gcgcccggcc gctatgagtg aaagccatga tcgttgacga cgttgccaag
     5881 ttgagcctcc ttgaagaccg gaagcccgac cacgcgctgc cgcgtccctt ctacaccgat
     5941 gccgagattt tcgatctcga tctgcgcaga gtctggtacc gcgactggct gttcgcgatc
     6001 ccgtcgtgcg agattccgca gaccggaaac ttcgtcacca tgcaggtcgg cgaatatgcg
     6061 atcgtgatcg tgcgcggtgc cgatggcgtg atacgggcct tccataattc ctgccggcac
     6121 cggggcagcc gcgtctgtac cgtgctgaaa ggcagcgcac cgaagctcgt ctgcccatac
     6181 caccagtgga cctacgagct cgacggtcgg ctgctctacg cccgcgacat gggccccgac
     6241 ttcaaggcgg ccgatcacgg gctcaagccg gtcgcctgcg tcgatgccgg tggcgtcgtc
     6301 tacgtctgcc tcgccgacca gccgccagcg atcgacggct tcgtcaacag cgcgcggcgg
     6361 tatttcgcgc cgcaccggct gaacgaggcg aagatcgcct acgagtcgac gatcgtcgag
     6421 aaggggaact ggaagctcgt catcgagaac aaccgcgagt gctatcactg cgccggcgcc
     6481 caccccgagc tgtgccggac ctaccccgac cggccaggct tcacttccat ggacgagagc
     6541 ggcgcgatcg agccggcggt gaccgcgcac tgggagaagt gcgaggccgc gggcctgccg
     6601 tcgaagttcg tcatcgcgcc cgaccaccag tggcgcttcg cccgcatccc gctgctcggg
     6661 ccgggcgaga gctacacgat ggacggcaag gcggcggtcc ctaccaagcg attgtcgaac
     6721 tcgccgttcg ccgacgccgg gtcgctgctg aacttccact atcccaacac gtggaaccac
     6781 ttcctcgccg atcactcgat tgtattcagg gtgctgccga tcagcccgac cgagaccgag
     6841 gtgacgacga agtggctcgt ccacaaggat gctgtcgaag gcgtcgacta taagttggag
     6901 aacctgacgc gggtctggat cgcaaccaac gacgaggacc gtcaggtagt cgaggagaac
     6961 cagcgcggca tcaactcacc ggccttcgtc ccgggaccct actccacgat ccaggaggac
     7021 ggggtgatcc agttcgtcga ctggtactgc cggaccatcg gcaaccgcgt cggcggcggc
     7081 ggccggctgg cggcggagta gactcgatgg cctcagacct gataccgcgc ctggtcggcg
     7141 cccggacctg gaaggatgac gagccgctcg agtgcgtctc ggtggtgccg gacggtccgg
     7201 gcacggtctc cttcaccttc cgctcgccgt cgggggcatg gttcgactat cgccccggcc
     7261 agttcctgac gctggagttg cccgtctccg gcgggccgat ccatcgcacc tacacgatct
     7321 cttcgagccc ttcgcggccg ctttcgatca ccctgaccgc gaaggcgtct gccagcagcg
     7381 tcggcacgcg ctggatgctc gacaacctgg cgcctggcac gcggatcaag gcgatcggcc
     7441 cgggtggcat attcgtcccc gacgtaccgg ccgaccgcaa gctgctcttg ctctcggccg
     7501 gctccggcgt cacgccgatg atgtcgatga cgacctaccg ctacgacctt ggcggcaagc
     7561 ccgacatctg tttcgttcag tgcgccaagc gaccttctga acttgtcttc cggcggcaac
     7621 tcgaggccat ggcgcgccgg gtcccgacga tcactctgca ttacgtggtc acccaggacg
     7681 atccgtatga cgcctggacc ggttatcgcg gcaccttcaa ccagctgatg ctcggcctga
     7741 tggcgcccga ctacctcgag cgcgaggtct actgctgtgg acccgagccg ttcatgcaga
     7801 cggtccgcga gatgctgatc ggcttcggct tcgacatgga tcactaccac caggaggcct
     7861 ttaccggacc gccggtgctg acgcccgccg acgcgccgga acgcgacgac gtcgtgccgg
     7921 acgccgaggc cgcctcggag gtgatcttca cgttgagtgg tcggtctgtg ccgtgcaccg
     7981 agacagacac gatcctcgcg gtgtcgcgga cggcgggcct caacatcccg tcaggctgca
     8041 acttcggggt ctgcgggacc tgcaagacca ggaagctctc cggggaggtg cacatggtcc
     8101 acaatggcgg catctccgag ggcgacatcg ccgcgggata catcctggcc tgctgctcgc
     8161 tcccgatggg gcgggtcgag atcgaggcat gacgtgacca cccgccgcgg gtaggccgac
     8221 gcgtcgccga cagacgacct ccaccgtgcc ccggaccgag gggcttggtc gctgattttg
     8281 cgcgtcgtga gttcctcgtg cgtgccccga gacgccatca ggacgactca tacggtttcc
     8341 gcgtgattgg gtcgggctgg tccgctttgg caccagtgga agctagcttg gcctcggacg
     8401 agatcgcggt cgttggcaag ggagacgcac ctgtcggcga tgatggcgtc gagatcggca
     8461 atggtcttga agtgccggtt gacgatcggc tcgtcgatag ggacccgaga gggtctcggc
     8521 cggctgcaac tcgggagtgt aggccggcag gtagacgagc ctgacgctct cggggacggc
     8581 gagcggcagg gtgtgcaagc cggcgccgtc gaggacgata atcctgtcgc gaccggctcc
     8641 cgcctcgcgg gcgaagaggg cgagggtctc ctcgaagagc tgcctgtcga cgccagtggc
     8701 gagataccag tggctctcgc cggtggccgg ggagacgaag gcagtgacgt agagccactc
     8761 gaagcggtgg tggccgcggg cgaccgggcg ctcgcccctc ggcgcctgga cccggcgcag
     8821 gaccggcttc aggccgatcc ggtgctcgtc ggtggcgcag gtctcgatca ccgcgccggg
     8881 agggcgctcc gcctcctctg tcacgatctc ggcaagcttt tttagaacgc cgcctgctcc
     8941 tccggcgtcg ccgactccac gtgccgcggc cgcgggctct ggatcgtcca gccgatcgcg
     9001 cacagcggct cccagccgcg ctgctccgcg acccggcgcc cgagctcggc cgccatgaag
     9061 gccgccacgt tccgtgccaa ccagaccccg ccgtcgtcgg gttggcgccg cgcccgctcc
     9121 cgcaccgccg ccatcatctc cggcgtcagc accgtcggcg ccgcaccgtt cccgcgccga
     9181 cggtcgccaa tgttgcccgg accaaaggcg ttgtagcgct ccagcagttg ctccacccgg
     9241 cgcagcgcga agctcgccag gcccgcgaca tcggcgcagg tccttccctg caccagaagc
     9301 cagatgacgt ggcaatgccg cgccggggtc gcgtcatcgc tgccccggta tcccgcctgc
     9361 aattcatcga cgctgagatg ccccgcaaca tgcgccatgc ctgccccact ttttggttgc
     9421 aggcatccta accctctcga cttcctccgg gaatccccaa ccgtccgagt caatcacgcg
     9481 gagaccgcat cagcagtcat cctcttgcgg tgtcggagaa gaagcctcct cgggcgcgtg
     9541 agccaatgta accgatcaga tcgcccaatc gtcgcgcggg cgcagacacc gcagtcaact
     9601 ggcagaaagg aggttcaatg tccgaaacgt ccaatgatcc cgcactggcg gccggcatgt
     9661 gcccggccga cggtcccacg gggatcatca acaccgacta tacgatcggc cgcgacaaca
     9721 tcgagggctc cctggggcct ctcggcttcg acatgcacaa tccggttttc gcaatttccg
     9781 cgctttcggt catcgcgttc gtcgccttca cgctcatgcg ttctgtttcc tggtatcacg
     9841 gcgccggacc gcccaacgtc tcagatcgga aaagccggtc aaggccgggt ttcgggacat
     9901 ggagcggaga agttctgcaa cgacgcgctg gccctgggct gcttctcctc gaacccggcc
     9961 atgatcgacg caaaagcccc gagggctgac cgcacgacac ggatttcaac cgctgtgatc
    10021 ggcatcacag tcgattcggg tcgtcataga tgccgaggga tgtcacgaag acgagcttgc
    10081 ggatgcccgc tgcttccatg gcggacacga tcgccgtcgc cggttcgtcc acgtcgccgc
    10141 tgaggttggc gtagaccacg tcttgaccct cgagggctgc ggcaagacgc ggcgcgtcga
    10201 ggacgtcggc ctcgacgacg cgggcgttgg ccggcacggc gtggagcttg cctgcatcac
    10261 ggaggtaaag cgtgagtcgg gcgtcctcgc ggtcggcgag catgtccgtc accagacggg
    10321 cgatctgccc cctgcgccga ggatcagcac gttcgtcact tcgttctcag ctccttggat
    10381 gaagcccggc ggttgcgggg cttcccgtta gccagccgcc acaggctcgc gcgcgtcggt
    10441 cgcgaccgtt tcgccaagat gctgccggaa gaatggcacc agacgctcca atgccttcgc
    10501 gaccggatca ggcttgtcgt agaggtcgta gtgcgaccag ccctccagcg agacgagctg
    10561 gcgatccctg gaggcattgg cgcggccgta gacctccatg ccatcgcgga aggcgccgaa
    10621 agcgccgacc ttctggccaa tcaccaccat caccggttgg gtcagcaagg tctcggcgaa
    10681 ggcgaaggcg tcccaggcga gggtcttctg cgcgtggctg aagagcatcc gggtcgagcc
    10741 gccgggtttc cggccgcggg gggtcttgta gtagtcggtc gcctcgaaga cgtcgcgctc
    10801 ggtcagccca gcgttcgtcg cagcctcggg gctcgcgggc aggagctcgt tcacctgtag
    10861 ctcgccatct cgggcctcgc ttgtgcgctg cgccgccatc gcgtcgagtg cgccgagcgg
    10921 gtcgtagctg gagaagccct cgcggaacag gcggccgagg ttgacgccgg tgatgctgac
    10981 caccgccttg atgcgcttct cggtcaaggt ggcgttgatc gcgtagccac ccccgccgca
    11041 aacgccgagg acgccgatgc gctcgacgtc gacgtacggc agggtaatgg cgtagtcgat
    11101 gacgcggctc acgtcctcga cgcgctgggt cgggtcctcg atccagcgcg gggttccgcc
    11161 ggactcgccc tggaagctgg cgtcgtaggc tatgaccacg aagccctcct tcgcgagtgc
    11221 cctaccgtag atattccccg aggtctgctc cttgcagctg ccgaaggggt ggacgctgac
    11281 gatggccggg tacttacgtc tctcgtcgaa tcccggcgga aagcggatgt cggcggcgat
    11341 gtgccacgcc atgtcggggt tctggatgcg aacggattgc attgttcatc cttctctgtt
    11401 ttcgccggac cggcgacggg cgccggtccg gtggggctcg ctcgcttact cggcgggcgt
    11461 gagctccggc gcgaggatgc gctggaagaa gggggcgagg acgccgacag cctcgttcac
    11521 gaactggtcg cggtcgtaca ggtccatgtg gttcgagccc tcgaccacgt ggaaatgctt
    11581 gtccgcgctc gcggcgcgcg aaaagaggtc gtcgctcatc cacttgctgc cggcgaccga
    11641 gcccgcgacg acctgcagcg gttgggtcag gaaggcctcc gccttgttgt aggcgtcgta
    11701 ggggatgatc tgcgtcaggc tgcgcgcggt ggcgtagccg ggggcggtcg ggtactcgca
    11761 gcgcggggtg tggtagtatt cccaggcctg ccgcagctcc tcgttcgggg cgtcctcctc
    11821 cttcagcggc gccagcggga tcgtggccgt ctctgcgccg gaggcgtcgg aagtgcgggc
    11881 gtcggagccg gccttgacgt agggggaggc gtccgcgtcc ttgacgttgt tctcccagcc
    11941 gttccggaac atcgagccaa tgttgacggc gctgaccgtc ccgaccgcct tgatccgggg
    12001 atcgttgatc gcggcgttcg cggtgtagcc ggcgccggcg cagatgccca ttgcgccgat
    12061 ccgctccttg tcaacgtagg gaagcgtcgt aagatagtcg atcaccgcgc tgacgtcctc
    12121 ggtgcggatg tgcgggttct cgagctgacg cggctcgccg ccgctctcgc cctgatgaga
    12181 ggcgtcgtag gcgatagtca cgaagccctc ctcggcgagc ttgcgtgcgt agagcccggc
    12241 ggtctgctcc ttcacgccgc cgcccggatg cgacacgacg accgccgggt agctccggct
    12301 ttcgtcgaac ccctccggga agttgatgac cgccgacatg gcgatcgtct ggttgttcga
    12361 gttcgggaag ctgatcttgc ccatcgtcgt cctccgtgtc gggtatgatc ccgaatgtag
    12421 ggatgaggcc gggcggcgcc ttgcccgttc cgacgcaatc cttgcccgat gtgcgggatt
    12481 tcagttcgac cgcagccgct gcacgtcccg ctgcggcggg gcgccgaaca gccggctgta
    12541 ttcgcggctg aactgcgagt cgctctcgta gccgacgagc acggccgccc gtcccgccgt
    12601 gacgctccgg ttcagcatga gctgccgcgc ctcctgcagc cggagctgct tctggtactg
    12661 cagcggactc atgccggcca ccgcgcgaaa gtgctggcgg aaggtcgagg gactcatgtg
    12721 ggcccgcgcc gccaggtcct caatgcggac catctgcgtg aagtttgcct tgagccaggc
    12781 gatcaccacg ccaatctgct cgcgcggcgc ctgttgcgca accagtcgcc tcaggagccc
    12841 gccatgtggc ccggcgagga gccgcaacac gatctcctgc cgaaccaacc cggcgaggtg
    12901 cggccggagc gggtcgtcga tcagcgctac cagccgctcg agcgcgtcga gcagccgcgg
    12961 gtcaagcgtc tcgatcgagg cgggcggcag cggtgtttgg cccggcaccg cgatcaccgc
    13021 gtcgatctcg ccggccgcga cggcgatcgc ccggatgtcg agcttcagca atagccccag
    13081 atagggcgcg cgaaggctcg cctccatgac atgcgaggtg atcggcagct ccatggtcgt
    13141 cagcatcgac tggcccggcc ggtacgcgac taccctgtcg ccaaggatca cctgcttcgc
    13201 gccctgggtg gtgacgatta gcccgaggcc gtaggtgcaa tcgaccggct cggtcggcgc
    13261 gtcccgccga tggagcgaca gctccggaac tgcggtcgcg acgtctcccg cccgcggcag
    13321 gtgcagcgct attgcccgcg ccagcggcgc cgtcgtcgcc tccgttcgca ccgcgatgcc
    13381 gttcccgtcc atgcctcacc ctaccgccgg cgccccttga atgtaagcgg cccggtcgat
    13441 ggcagatcga tggcaggggt aggcgccgcc tatggcccgc gcgggcggat tgtctcatgg
    13501 cctcgcccgc ggcatttctt tcccaggacg gaaccggcgc cgcgaccggc ctcggcggac
    13561 cggcggcaga acgcgggagg gaagatctta tgcggggcat ggtgttcagg ggagaccgga
    13621 aggtcgagct cctcgacttc gacgatccca cgcccgggca gggcgaggca gtcatcgaga
    13681 tcaaggcctc gggcatgtgc ggcagcgacc tgcacttcta ccgcaacaac cccgccgagg
    13741 tgatccgctc gctcggcttc aaggatctcg cctcccgcgg catcgacgag aacactccga
    13801 tcatcggcgg ccacgagcct tgtggcgtcg tcgccgcgct cggccccggc gtcgacccga
    13861 aggccttcgg ggtcggagac cgggtgatgg tgttccatta cgagggctgt acccactgcg
    13921 atcactgccg gactggctgg acgcagatgt gcgaccacgg cgcgatcgtg cacggcgtca
    13981 tcgcccacgg cgcccacgcc gactacatga aggtgccggc ctcctgcctc gtgccgatgc
    14041 ccgagggcct ctccttcgcc ggcggcgccg cggtcgcctg cggcaccggc accgcctacg
    14101 gcgcgctgac ccgcctcgac atcagcgccc gcgacacgct cacggtcttc ggcctcggcc
    14161 cggtcggcct gtcggcggcg cagctcgcag cggcgatggg gatcgaggtg atcggcgtcg
    14221 acatctcggc cgaccgcgtc gagcgcgcaa aggacttcgg cgtcgcgcac gcgatcgacg
    14281 gcagcagcac cgatccggtc gaggcgatcc gcagcctcac ccgcggcaag ggggtgacct
    14341 gcgccatgga ctgcgccggt ggcgaggccc cgaagcagca ggcagtgcgc gcgaccgcgc
    14401 cctggggccg gatcgcgctt gttgcagtcg gcggcaatct gaacgtcgat gcgatgaagg
    14461 acctgatcgg caagcagcgc acggtgatcg gatcctacac cttttccagc gttgggatga
    14521 aaggctgcgc ggagttcatc accgaccacg gcgtcgaggt cgataagctg ttcacggacc
    14581 agtggcgcct cgaccaggcg gaagaggcct atcgcagctt cgacaagcag gcaggcggca
    14641 agggcgtctt cctgttctga ccctcgaagg gcttccgcct tctccgagcc aattccgcgc
    14701 acgccttccc cgggcgcgtc ggcctttctg ccggagccga tgcgccgcgc agctacaggg
    14761 tccgcctatg ccacccccca cgcgcatcct gggagggaag cgtcgcggcc atgtgctaaa
    14821 ctccgaacga gcacttcgtc agatcgaaaa cgagctgcac cggtcaacac acgggaggaa
    14881 cgcgatgttc gccaagcggg tcaccgccgc cattcttgct gcctcgacgc tcgccggcgc
    14941 cgcttccgcg caggaaacgg cgacgctgaa gttcgcccac gtcttcccgg ccgagcacta
    15001 cctctggacc cacgggggca aggtcttcgc cgacgcggtc gccgaggcga ccgacggccg
    15061 catcaccttc caggtcttcc cggccgccca gctcggcaag gactatgtgt cgctcctgaa
    15121 gtcgaacctc gcggagagcg cgctcctggt cccctcctac acaccggaaa gcctgtcgct
    15181 cacctcggtc tccgagctgc caggcctcta ctccgcctcc tgcgaggcga ccgcgaagct
    15241 ctgggagatc gcccgacccg gcggcgtcct gaacgcggac gaatacgccc cgcaaggcct
    15301 gcacgtcctg ttcgtctcca ccctgccgcc cttcaaggtg ctgacggcgt cgcagcccat
    15361 cgccgagatc gccgacctcg ccggcctcaa gatccgcgcc aatggcgccg ccatggacaa
    15421 gaccgtccgc acccttggcg ccacgccgat ccaggtcact gcgggcgaac tctacgactc
    15481 ggtgacgcgg ggcacgatcg acggcggctt cttccccttt cactcggtcc cgacctatca
    15541 tctcgaggag gtgttcggct tcggcgtcga gggcgctcag ctcggttcgg gcagcgtggt
    15601 cttcgcgatg agcgaccgcg cctggtccaa gctcggtcca gacgaccagg cagcgctgac
    15661 cgaggccgcc gcagcggcgc agcagaacct ctgccagtgg ctcgacgccg aggaggcccg
    15721 cgtccgtgac ctgctcgtgg ccgagaacgg cctgcaggtg acgagcctct ctcccgacca
    15781 agccgtcgcc tggcaggagg ctgtcgccgc ggtcgccacc gactgggccg cggaccttga
    15841 caggctcggc cgcccgggca ccgcggtcct cgaagccttc cgcgacgccg cgccctgagg
    15901 agcccgtcat ggtttggcaa ggacactacg accgactgtt catcggcggt gactggatcg
    15961 ccccgtcgac cggcgcgagg atcgccgtca cctcgccgct caccgaggag gtgatcgccg
    16021 aggtgcccga tgcggcccgc acggacgtcg accgcgccgt cgccgccgcc cgcgccgcct
    16081 tcgacaccgg cccctggccg cgcatgccgc tcgacgagcg catggccgtc ctggcctgct
    16141 tccgcgacat catcgcggag cgccgcgacc aggtcgccca actcgtcacc gacgagatgg
    16201 gctgcccgat caccctctcg aagggcatgc aggcgaccgg gccgaagctg ctgctcgacg
    16261 ccttcctcga actcgcgccg gggtatccgt ggcaggacgt gcgccgctcg accaccggca
    16321 ccggcctcgt gctgcgcgag ccgaagggcg tcgtcgccgc ggtggtgcct tggaacgcgc
    16381 cgatactgat cgcggcgatc aagctcgcgc cggcgctcct ctccggctgc accatgatcc
    16441 tgaagcccgc gccggagagc ccgctctcgg cctacctcct cgccgagatg ctggccgagg
    16501 ccgggcttcc cgccggcgtc ttcaacgtcg ttcccgccga ccgcgaggtg agcgagtacc
    16561 tcgtgctcca cgagggcgtc gacaaggtga gcttcaccgg ctcctcggcc gccggccgcc
    16621 gcctgggcgg catttgcggc gagcggctgc gccacctgac gctggagctt ggcggcaagt
    16681 ccgcgggcat cgtcctcgac gacgccgacc tcccctcggt catgcaggcg atccgggcga
    16741 cgtcgctgcg caattccggc caagtctgct ccaacaagac ccgcatcgtc gtctccaggc
    16801 gccgtcgcga cgagctcctc gacgcgctgt cggcgatgat ggccacgatg cccgtcggcg
    16861 acccgaacga cccggcgacc gagatcggcc cgatggtcag ccaccgccag cgcgagcggg
    16921 tggagggcta catcgcgagg ggccgcgagg aatctcgcct cgtcctcggc ggcgggcgac
    16981 ccgccggtct caaccgcggc tggttcgtcg agccgacgat cttcgccgac gtggaccgca
    17041 acgcggtgat cgcccaggag gagatcttcg gcccggtcct ggcggtccag acctacgagg
    17101 acgaggacga cgccgtggcg atcgccaacg actcgaccta cggcctcaac ggtgccgtgc
    17161 actcggccga tcccgagcgc gcgctccgcc tcgcccgccg catcgagacc ggcaccgtcg
    17221 agatcaacgg cgccggcgtc ggcttccatt ccccgatcgg gggcgtaaag atgagcggca
    17281 ttggccgcga ggccggcccg gagggcttcg acgcgtacgt cgatatcaag tctatcggcc
    17341 tcccagctgg cctcgcggac aagttcgcca actgacccgc tgccgcccaa cgggaagggc
    17401 ccgcctcgct gcgggcccga agtcgcgcgt cgcggcggcc gtcaatcgcc cgacgcgccg
    17461 cttgtgcggt aggcctcgag gatctccgtt cccggccgac cgttctcgtc cagccgcttc
    17521 gcccaggccg cggccacccc ggccatccgc tcgttccagg ctgccgtgtc cgactccggg
    17581 aaggagacga ccgtcagccc cttctcggca acgtagcgct cccggatcgc cgcctcgtcc
    17641 gcgtcctggt attcgcagac cgcgcgctgc gccgtcgcgg ccgcctcggt catcgccgtc
    17701 tgtatctccg gtgtgagacc cgcccaggtg tccgcatcga tcgcgtagag cactgcgagc
    17761 gatcccgcga acacgttgtc gaccgcatgg tgcatgacct cgccgaggct gtagggcggc
    17821 atccccacgt aggcgtagat cgcaccgtcc accgttccgc gcgagacaga gtcgtagaac
    17881 tcggacgcct ggacttggat cggcgtcgcc ccgaccgcca ccaccgtatc gccctgtgcc
    17941 gcgcccaccg tccgcaactt gaggccggca aggtccgcga ggccagttac ggccgtcgac
    18001 ccggtcataa ccttgtaggg tggcggcacc gcgatgaaga gaacgcgcag gccgagcggc
    18061 tcgtactcgt gctggtcaag cgcgccgccc tcgcgcacca gcggccagac ccggtccgcc
    18121 ccctcgcagg cgcccgaaat gaagccgggc agctccacga ccccggtcag cggtagcttt
    18181 tctggcgcgt acggcgccgc gatcaccgcc atctgggcga ggcctgagtc gaggagcccc
    18241 agctggtctt tgccaagctg gccggcatgg aaggcctcga ccgtcaccgc gccgcttgtc
    18301 gcttcgtcga ccgcatcgac gaagaccttg cccccgttct gccagaggaa atggttctcc
    18361 gggaaggtgt tggaaagccg gagcgtcacg tcctgcgcgc ccgcggcaat ggtcgtgcca
    18421 caaaggacgg cgcaagtagc cgccccggcc agaagccggt tcatgctcat tgtcttctcc
    18481 ctgatgctcg ccgccccgac cagccggggt cagtcgaggt tcatgtagac ggccttcgga
    18541 tagaggaagc tgtcgacatg ctcgcgagcc cccttccaac cgtagccact catcttgtgc
    18601 ccgccgaagc caatcgaagg atcaagcacg ccgtagcaat tcacccagac tgtccccgct
    18661 cggactgcgc gtgagacctt gtgtgcggtc gagaggttgc gggtccacac cccgccggcg
    18721 agcccgaagg gtgtgtcgtt ggcgatccgg agcgcctcat ccatgtcgcc gaacggaata
    18781 atcgaggcga ccggcccgaa gatctcctcc cgtgcgattg tcatgtcgtt cgaaactccg
    18841 gcgaagaccg tcggctcgac aaagaagccc gaggcgagat ccccgcccag ccgcgtcccg
    18901 ccggccgcca accgcgcgcc ctcgcgggcg ccgacctcca aatagccgag cacccgatcg
    18961 agctgccgct gcgagatcag cggcccaagc tgggtgtcgg gcgcgaggcc attgccgaca
    19021 cggatggacc tggtgaaggc ctgcatccgc tccacgaact cgtcgtggat cgcgcggtgc
    19081 acgaggatgc gcgtgccggc gacgcagacc tgtccgctgt tggtgaagac ccccatcgcc
    19141 gcgcccggca ccgccttgtc gaggtcggcg tcggcgaaga cgatgtcagg cgacttgcca
    19201 ccgagctcga gctgaatgcg cttcatgttg ccggccgagg cccgcacgat gctctgcgcc
    19261 gtcaaatagg agccggtgaa ggccacccgg tcgacgtcca tgtgctcggc cagcgcctgc
    19321 ccggcctccc ggccgtagcc agtgacgacg ttgatcacgc cctcggggac gcccgcctcc
    19381 agcatcagct cggccgtcct gagcaccgac agcgaggcgt cctccgccgg ctttatgaca
    19441 gcggtgcaac cggtcgccag cgtcggtccg aagatccacc actgcccgat gagcggcccg
    19501 ttccagggaa tgacaccccc gaccaccccg accggtgcct tgtgcttcag cgtcaggaag
    19561 tcgcctggca gcgagttcgg cggcgtctcc acggtcgccg cgccggtctg cgaggcgtag
    19621 aactgcagca cctgcgacgt ccattcccga agcggccggg tccgcgcgag aggcgcgccc
    19681 atgtcgaggg tctcgatcag cgccaactcg tcgaaatggt ccatcagcac gtcgtgtacg
    19741 cgcaggatca acttgcggcg ctcgtgcggc gtccaccggc tccaggagcc ctcgaacgct
    19801 cgccgcgccg ccgccaccgc cagatccacg tccgcggcat cgcccgcgcc gatccggcac
    19861 agcgtctcgc cggtcgccgg attgatggcg tcgatcgtgc ggcccgaccg gggcgcgacc
    19921 cattgcccgc cgatcagcag gcccttggtc ccggcgacga aggcgggcac ctcgaccttc
    19981 ggcaactcca tcgtggtcat gcgctcctcc ctttatcgtc agcctgcggc cgatgcccgt
    20041 ctccggcaag cgggccggtt ccgcccccgg cggccgcgat ggcggccaga ggcagcgccg
    20101 atggctatcg ccgtgccccg gccatcgcgg cgaccagcgt gcagggcctg tcgctgcggt
    20161 tgctccaagt gtgcttggtg ccgcgctgca cgaaactgtc gccggccttc agcagcacct
    20221 cgcccgtctc gagcaccgca tatatctcgc cggaaaggat ggtgacgagg tccaccgtgt
    20281 cggtctcgtg cagccccgcg atcgcctcgt cctcgacatg cgacccgccg tcccccgcag
    20341 ccgtcagcgc gtccttgtag ccggccgcgg cgtcccagga cgcatctggc ggaaaggtgg
    20401 cgaggagata gacgaagccg gtccgcggcg gcgcgatcga cacgccttcc gagccatcct
    20461 cggccagcac cggcgtcggc agcgcgccga cctgccagat ctggttgatg gtgaagcccg
    20521 gcgtttccag ccgcgacgga gtctcgccgt cgctggctat cgtcgaacgt ccggccgtgt
    20581 ccagacccgc gacgacccga cgggcccgca tttcggtatg cttttctgac actctgcttc
    20641 ctccctcgat gcggccgcgg ggccgcgctt ggtcgtggtt gcctcggttc gccggacgtt
    20701 gaccagctgg ccgaagacgg gccggcgcga gcgcgtggcg cccgcgcggt cggcgggctc
    20761 caggccgccg accgctccag cgcgagagcg atgccctggc cgccgccgat gcacatggtg
    20821 acgagcgctc ggctcccgcc gatgcggtcg agctcgtgga gcgccttcac ggtcagaatc
    20881 gccccggtgg cgccgagagg gtggccgagc gcgatggcac cgccgttcgg gttcaccttc
    20941 gccgggtcca ggccgaggcc gcggttgacc gcgatcgctt gcgctgcaaa cgcctcgttt
    21001 gactcgatca cgtcgaagtc ggcggccgtc atcgcgagct tgtcgaggag cttgccaacc
    21061 gcggtgatcg ggccgacgcc catgatgtgc gccggcaccc cggcgacggc gtagccgagt
    21121 atgcgcgcgc ggggcttcag ccccgcctta ttggcggcgg cagcggtcgc gagaacgacg
    21181 gcggccgccc cgtcgttaag gcccgaggcg ttgccggcgg tgacagtgcc gtccttcttg
    21241 aaggcaggtc gaagcttggc gagcgcctcg ggggtcgtcg ccttcggatg ctcgtcggtg
    21301 tcgaagccga cgatctcgcg cttcaccttc acctcgaccg gcacgatctg gctcgcgaag
    21361 cgcccctcaa ggatcgcgcc ggcggcgcgg gtctggcttt cgagcgcgaa cgcgtcctgt
    21421 tcctcgcggg tgatcccgac atcggccgcg acgttttcgg cggtgacgcc catgtggccg
    21481 gtacccatcg ggcaggtgag ggcaccgagc atcatgtcca tggccgcggt gtcgcccatc
    21541 ttctgccccc agcgcgcccc ggtgagcgcg tagggcgagc gtgacatgct ctctgccccg
    21601 ccggcgaggg cgaagtcggc gtcgcccatc ttcagcgcct gcatgcagga aacgatggcc
    21661 tgtgcgcccg atccgcacag cctgttgacg ttcatcgccg gcgcggcgtc cggcacgcca
    21721 gcatcgagcg ccgcggcacg ggagaggtac atgtcccgcg gctcggtgtt gatgacgtgt
    21781 ccgaagacca cttggccgat ctgccctccc tcgacgccgg agcgcgccag cgcctcccgc
    21841 gccgcggtgg ctgcgagcgc gatcggcggg atcgcggcga ggctgccgcc gaaggtgcca
    21901 atcgcggtgc gaaccccccc gagtattacg atttcgtccg gcatggtacc tcttccgttc
    21961 cctggatcga ttctctgtga gaccgggtcg ccgctgaagg cgccccgacc gtggctcccc
    22021 ggtgcatgcg cgcgcgctgc cccttcgagg gagctcacat gttcggatag ttcgggccgt
    22081 ccccaccctg cggtgtcgtc cagtcgatgt tctgcagcgg atccttgatg tcgcaggtct
    22141 tgcagtggac gcagttctgg aagttgatct ggaaccgagg gctggacccg tcggtgtcgc
    22201 gcagcacctc gtagaccccg gctggacaat agcgctgcgc tggctcggaa tatttcggca
    22261 ggttcaccgc gatcggcaac gaggggtcgc gcagccgcag gtggcagggc tggctctcct
    22321 cgtggttggt aaaggagaag gcgacgttgg tcagccggtc gaaggagagg acgccgtcgg
    22381 gcttcgggta ttcgatgggc gcgaagtcct tcgcctggcc agtcgcctcg gcgtcggact
    22441 tgccgtgccc gagcgtcccg aacaggctga ggccggtcag gctccgcgtc cacatgtcga
    22501 ggccgccgaa ggccagtgac gcccagagcc cccacttcga ccacatcggc ttgacgttgc
    22561 gcaccggctt caggtcgcgg ccgatctcgc cctcgcgcac tgccgtctcg tagtcctcga
    22621 gctcgtcgcc ctggcgccct gccgcaagcg cggcgtgcgc ggcctcggcc gccgcgatgc
    22681 ccgacagcat ggcgttgtgg ttgcccttga tccgcggcac gttgacaagc ccggctgagc
    22741 agccgagcag cgcaccgccc gggaaagcga gcttcgggat cgactgccaa ccgccctccg
    22801 agattgctcg cgcgccgtag gcgacgcgct tgccgtcccg gagcacgtcg gcgatcagcg
    22861 ggtgatgctt cagccgctgg aactccatgt acggagagag atgcgggttg cggtagttca
    22921 ggtgcacgac gaagccgacc agcacctggt tgtcctcgaa gtggtagagg aacgacccgc
    22981 cgcctgcgtt cgacccgagg ggccagccca tcgtgtgcac gacggtgccc ggccggtgga
    23041 cctccggctg cacctcccag atctccttca tgccgaggcc gaacttctgc ggctcgtatc
    23101 cttccgagag gccgtagcgc gcgatcgcct gcttcgacag cgacccgcgc acgccttccg
    23161 cgaggaagac gtacttgccg cgcagctcca tccccggctc gtagcccggc ccgggcgtgc
    23221 catccgcctc gcggccgaac tcgccggcca cgacgccggc cacgcggtcg ccgtcgtaga
    23281 ccaggtccga gcaggccatg ccagggaaga tctccacgcc gagtccctcg gcgataccgg
    23341 cgagccagcg gcagacgttc gccatcgaca cgacgtagtt gccgtggttc gacatcagcg
    23401 gcggcatcgg ccacgtcggg atgcgcacct tccccgaacg tccaagcagg tagaagcggt
    23461 cctcggtcac ctcggtgtgg atcggcgcgt ccatctcgcg ccagtccggc aggagggcgt
    23521 cgagcccgga cgggtcgagc accgcgcctg acaggatgtg cgccccgacc tccgagccct
    23581 tctccaggag gacaacggac agctcggggt cgagctgctt cagccggatc gccgccgcga
    23641 gccccgctgg gccggcgccg acgatcacca cgtcgtagtc catcgcctcg cgggtcatgg
    23701 gtcctcctgt ggcgcggaac cgcctccggc tccgcgtgcg atttgggttc gaatcccgga
    23761 tagcttcggg gccggagtcc cggccccggc cgatcagctg agcttttcct cgagctccgg
    23821 cacgatttgg aagagatcgc cgacgaggcc gtagtcggcg acctggaaga tcggcgcctc
    23881 ctcgtccttg ttgatcgcga cgatgacctt cgagtccttc atgccggcga gatgctggat
    23941 cgcgccggag atgccgaccg cgttgtagag ctcgggcgca accaccttgc cggtctgccc
    24001 gacctgccag tcgttcggcg caaagcccga gtcgaccgcc gcccggctgg cgccgaccgc
    24061 ggccccgagc ttgtcggcca acttctcgat cacccggaag ttctcctcgg agccgacccc
    24121 gcggccaccg gagaccacga tcttcgcgct ggtcagctcc ggccggtccg aagtcgccac
    24181 cttgtcctcg acccaggacg ataggccggg gtccgaaacc gcggtcccga tctcgaccgg
    24241 ggccggcgcc tgctcgccgg tggctccgaa ggaggccggg cgcacggtca ccagcttctt
    24301 cgggtccgag ctctgcacgg tctggatgcc gttgccggca tagatcggcc gctcgaaggt
    24361 gtcggccgcg acgacgccgg tgatgtcgga gaccaccatc acgtcgagca gcgccgccac
    24421 ccgcggcgcc acgttcttgc cgctcgtcgt cgccgggaag acgatgtgct cgtaggggcc
    24481 ggccatggac acgacgaggt cggtcagcgg ctcggccagc cgatgcccgt aggcctcgcc
    24541 ctcgacgtgg atcaccttct tcacgccgtc gagcttcgcc gccgcctcgg cgaccgcccc
    24601 gacattctcg ccggccacga gcacggtgat gtcaccgccc atcgcctttg ccgcggtcac
    24661 cgtcttcgct gtcgcgtcgt tgaggctctt gttgtcatgc ttcgccagga tcaggacggc
    24721 catcagatca ctcccgcctc ggtcttcagc ttcgccacca gctcgtccac cgagccgacc
    24781 ttgacgccgg ccttgcgcgt cgccggctcg accgtcttca ccaccttcaa ccgcggcgcc
    24841 acgtcgacgc cgtagtcggc ggcggtcttc tcctccagcg gcttcttctt cgccttcatg
    24901 atgttcggca gcgaggcata gcgcggctcg ttcaggcgca ggtcggtcg
//
LOCUS       DAOTFI010000031        24913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2333_length_24913_cov_7.196516, whole genome shotgun sequence.
ACCESSION   DAOTFI010000031 DAOTFI010000000
VERSION     DAOTFI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 24913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24913
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_24913_cov_7.196516"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5558_04685"
     CDS             complement(<1..220)
                     /locus_tag="P5558_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23637.1"
                     /translation="MLVLWHARDSATIAGLDLTQLLGALGQIGEQCVEAVVLGGLGRD
                     RLGLQFIGNDIGSRAWRRLVAWYQALDPD"
     gene            503..790
                     /locus_tag="P5558_04690"
     CDS             503..790
                     /locus_tag="P5558_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRY23638.1"
                     /translation="MAAAPAASGELTASYRLQLLAVRDEAAAAAAWSDLQRQHPSVLG
                     GLRSRVEQANVGGNTFFRLQAGPFQGRDAADRACRVLQQAGSDCFIVGPTS"
     gene            787..1827
                     /gene="nagZ"
                     /locus_tag="P5558_04695"
     CDS             787..1827
                     /gene="nagZ"
                     /locus_tag="P5558_04695"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRY23639.1"
                     /translation="MRASAGTSQARAAIVGIAGTALEPAERRLLARNPPAGFILFQRN
                     CESRSQLLALTNELRALFPDRWVPILVDQEGGRVQRLGPPHWPQLWPARMLGRLAERD
                     RAAGEAAAALQARAMATILREVGIDVVCAPCLDLLMPETTGAIGDRAFAADPALVAGL
                     GRITADTLMASGVLPIIKHLPGHGRATCDSHLELPRVVAEWAILDASDWQAFRPLADL
                     PLGMTAHIVFAAIDPDRPATQSPRVIAEVIRGAIGFQGLLLSDDLSMQALSGPIEERA
                     SLALAAGCDLALHCSGDAAEVDAVLRTVSDLEHASMTRLQSLRPGPAAGEALDDLVTT
                     LAGLLEAEVGRA"
     gene            1820..2509
                     /locus_tag="P5558_04700"
     CDS             1820..2509
                     /locus_tag="P5558_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRY23640.1"
                     /translation="MPDLVALGHTLSVWFLPVVIAITMHEAAHAWAADKLGDDTAKSL
                     GRLSFNPIRHVDPFGTLVLPGLLLLVGAPFLFGWAKPVPVAFHRLHNPKRDMIWVALA
                     GPGINMVMALAVALLMHGFVGTQSEIGQWVWQNLDNALLINVVLACFNMLPLLPLDGG
                     RVLTGLLPVPLAVKFARTERYGLFILLGVVFVLPLLARELGFAINPLAAILLPMVQTV
                     HSLVLLLTGWS"
     gene            2509..3240
                     /locus_tag="P5558_04705"
     CDS             2509..3240
                     /locus_tag="P5558_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRY23641.1"
                     /translation="MSALSPDRFVVDLDGFEGPLDLLLELARAQKVDLSRISILALAD
                     QFLAYLEEARARELSLAAEYLVMAAWLAFMKSQLLLPREAREDEDVDAMAEALAARLR
                     KLDAIRRAVAWLDLCPELEVARYARGAPESAPVRHEDHWRADLAGLMESYARLMARRR
                     QQSIRLVPRRAMSVEAALERLSRLLTGREWQDLAGFLPPGLADVFLGRSALAASLVAS
                     LELAKRGTIEIDQAAPFGPIRIRRR"
     gene            3237..3863
                     /gene="scpB"
                     /locus_tag="P5558_04710"
     CDS             3237..3863
                     /gene="scpB"
                     /locus_tag="P5558_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRY23642.1"
                     /translation="MSDEGEAIDAKLVVEALLFQSREPLDEALLQAHVRDGRHVLAIL
                     EELAADYAARGVRLERHGRQWAFRTAASVAPHLERVESRRRRLSKAAMETLAIIAYQQ
                     PVTRAEIEAVRGVPVARGTLDQLVEAGWVRPKGRREVPGRPVTWVTTPGFLDQFDLSS
                     LDDLPRLDELEAAGLFRTAGGGGSAPDDATAEAHGQADIELPPGVAGT"
     gene            3961..4188
                     /gene="tatA"
                     /locus_tag="P5558_04715"
     CDS             3961..4188
                     /gene="tatA"
                     /locus_tag="P5558_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRY23643.1"
                     /translation="MSIGIWQVVLILVIVLILFGAGKLPKVMGDVASGVKNFKKGMKD
                     EDEEQTPRALSEEATSPEMKMDRTKDEAAKS"
     gene            4229..4684
                     /gene="tatB"
                     /locus_tag="P5558_04720"
     CDS             4229..4684
                     /gene="tatB"
                     /locus_tag="P5558_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HRY23644.1"
                     /translation="MFDLGWSEMAVIMLVALIVIGPKDLPKVAKTIGHWTGRARALAR
                     DFQRSIDDMAREAELDEIKKSIDRTGRIDLRKTISDAVDPKGELARAFDVEDKAKPGK
                     VGKTAPAKPRIEAPAAPAGPPAAVADDTATSEPDKDAATLSDGATTPRS"
     gene            4736..5506
                     /gene="tatC"
                     /locus_tag="P5558_04725"
     CDS             4736..5506
                     /gene="tatC"
                     /locus_tag="P5558_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRY23645.1"
                     /translation="MPLLDHLLELRNRLMYSAAAILLGFVVCYFFSEQIYAFLVRPLA
                     AVYEGQTGRRMIYTSLTEAFFTYLKVAFFAGAFITFPFVATQLYLFIAPGLYRNEKQA
                     FLPFLIATPVLFFLGGAMVYYVIFPLAWRFFVSFETAGGAGTLPIELEARVGEYLDLV
                     MKLIFAFGLCFQLPVALTLMGRVGLVTADGLARNRKYAVVGVFVVAALLTPPDIISQL
                     GLALPILALYEISIHLVRLAEKKRDEAQAAEEAAADDD"
     gene            complement(5564..5638)
                     /locus_tag="P5558_04730"
     tRNA            complement(5564..5638)
                     /locus_tag="P5558_04730"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5603..5605),aa:Glu,seq:ctc)
     gene            complement(5679..6866)
                     /locus_tag="P5558_04735"
     CDS             complement(5679..6866)
                     /locus_tag="P5558_04735"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRY23646.1"
                     /translation="MAILDGERLLVPGALPGERWQVRRGVRRGWLDPVACLVASTERA
                     RPPCGHFGMCGGCTLQHLRPEAYAELKRRHVRGALRARHVEVPEPIVIHRSPLGSRRR
                     LRLGFDKGGRLGFRQRHSSAIVAIESCPIARPGLVALFAPLRQLLPRLDCARKGGEVT
                     LTALDSGVEVVLHLAVQPSLAERETLAAFAEAEALVRLAITVDGAEPEPVAARLPAAL
                     TIAGLPVSLPPAAFLQATAEGEACLQAFVVRHLEGARRVVDLFAGVGTLSLPLAGPNV
                     RIRAFDVAAELVAACRHPRIEASCRNLFQAPLAPSELAGCDAIILDPPRAGAEAQVPE
                     LARSGVDRIVYVSCAPASFARDAAVLVAGGYRLAALEIVDQFVYTAEVELAAFFTRQP
                     KGA"
     gene            complement(6938..7234)
                     /locus_tag="P5558_04740"
     CDS             complement(6938..7234)
                     /locus_tag="P5558_04740"
                     /inference="COORDINATES: protein motif:HMM:NF039467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6476 family protein"
                     /protein_id="HRY23647.1"
                     /translation="MERYLGIIKLFNIVAAVVIVLGVVALVWLMVERGRGDRAAVDSG
                     SIVLPTGFRVIEMSATSDFVFLLLGDDDDALLLLRLDPRDPASSASFVVETARP"
     gene            7270..8220
                     /locus_tag="P5558_04745"
     CDS             7270..8220
                     /locus_tag="P5558_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY23648.1"
                     /translation="MAEPALVEITDGEAGRRVDAVLAERLDDLSRSRLQALLRDGHLT
                     VGDAVVTEGKRRVKPGECYRLVVPPPSPSGLVAEDRPLDVVFEDDDLLVVDKPAGLVV
                     HPSVGHDHGTLVHALLAHCGASLSGIGGVERPGIVHRIDKDVSGLLVVAKHDRVHQGL
                     AGQFTVHSVDRLYEAVVHGLPSPAAGTIDQPIGRHPVDRKRMAIVPAGKRAVTRYRLL
                     EAGGTLIARLEVALETGRTHQIRVHLSSLGHPLLGDRLYRSRRRQELPAPLREEIEGL
                     GRVALHARVLGFTHPRTGERLRFESPPPAIFDRLLALARA"
     gene            8372..9274
                     /gene="rpoH"
                     /locus_tag="P5558_04750"
     CDS             8372..9274
                     /gene="rpoH"
                     /locus_tag="P5558_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRY23649.1"
                     /translation="MASLPTVPLEGSGLSRYLEEIRRFPMLEPEQEYMLAKRWRENDD
                     VAAAHTLVTSHLRLVAKIAMGYRGYGLPMNEIISEGNVGLMQAVKRFEPERGFRLATY
                     AMWWIRAAIQEYILHSWSLVKLGTTAAQKKLFFNLRRLKSQMQAIEEGDLSPEAVKKI
                     AETLAVTEQDVIDMNRRLEGPDHSLNATLRAESDMEWQDWLVDDRATQDAMLAEDDEL
                     DHRREMLTSAMHVLNDREKHILTERRLKESPTTLEELSGQYGISRERVRQIEVRAFEK
                     LQKAIRGMEATRTAEAAAATIPSG"
     gene            complement(9315..9890)
                     /locus_tag="P5558_04755"
     CDS             complement(9315..9890)
                     /locus_tag="P5558_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRY23650.1"
                     /translation="MRTDDANDNHLAGMLLVAMPGMPDPRFERSVIYLCAHSSEGAMG
                     IVVNQIAENVEFTAIVEQLGIEPTTDAANLKVHVGGPVESSRGFVLHTQDYVHENTLL
                     IDAEFALTATVDVLKAIASGTGPRQRVFALGYAGWAAGQLDAEIQKNGWLIAPPDEHI
                     VFELDDTQKWTGALNKIGVDPSLLSSTAGHA"
     gene            9984..10469
                     /locus_tag="P5558_04760"
     CDS             9984..10469
                     /locus_tag="P5558_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRY23651.1"
                     /translation="MTIKTGDRLPDATFKRNNAGDITDVTTMELCAGKKVVLFAVPGA
                     FTPTCHAKHVPSYLQHHDALKAKGVDTIACIAVNDPFVLGEWAKSTGTADKIVMLSDG
                     NAEFTRAIGLDFDGSGFKLGTRSKRYAMVVEDGVVKTLAVEEAPPMMEVSGADKILAA
                     L"
     gene            complement(10476..10958)
                     /gene="rnhA"
                     /locus_tag="P5558_04765"
     CDS             complement(10476..10958)
                     /gene="rnhA"
                     /locus_tag="P5558_04765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRY23652.1"
                     /translation="MASTDALRTEPAATDVVEIHTDGGCSGNPGPGGWGAVLRWRDHE
                     RELSGHEPATTNNRMELMAAIQALESLKRPMRVRLVTDSAYLRKGITEWLAGWKAKGW
                     KTADKKPVKNVDLWLRLEAAARRHEIEWAWTRGHAGDPLNERADGLATAAIRAAKAER
                     "
     gene            complement(10945..11907)
                     /locus_tag="P5558_04770"
     CDS             complement(10945..11907)
                     /locus_tag="P5558_04770"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRY23653.1"
                     /translation="MAVYTSVPVPALEALLAQYGIGEARTLEPIAEGVENSNFRLDTT
                     TGRYILTIYEKRVDPADLPFFLTLMDHLAKHGIACPVPIHDRHGRSLQRLCGKPAAIV
                     SFLDGRSPKRIDAARCAALGATLASLHQAVGDFAGYRANALSVTSWRPLFEACRQSPT
                     PPSAELADTIAQALDDLERNWPQALPSGVIHADLFPDNVFFAGDRVTGVIDFYFACSD
                     RFAYDVAICLNAWCFEGRAEFNLAKSRALLAGYQAVRPLEPAEIEALPVLCRGAATRF
                     LLTRLFDWLNQVDGALVKPKDPMEYARKLAFHRRVEEPAAYGLD"
     gene            complement(11999..12970)
                     /gene="ispH"
                     /locus_tag="P5558_04775"
                     /gene_synonym="lytB"
     CDS             complement(11999..12970)
                     /gene="ispH"
                     /locus_tag="P5558_04775"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRY23654.1"
                     /translation="MPPTDRPHLTICLANPRGFCAGVDRAIAIVKEALRKNGPPIYVR
                     HEIVHNRHVVEELRGLGAVFVDELDEVPEDAVVVFSAHGVPKRVPAEARRRRLIFADA
                     TCPLVSKVHREVERHHADGLTVLLVGHAGHPEVEGTMGQVDDGAVLLVETVADAENVM
                     VPDPDKVAYATQTTLSLTDTAAIVTVLRQRFPAIAGPRSEDICYATTNRQEAVAAMAP
                     GSDLVLVVGAPNSSNSVRLVEVARQNGAARALLIQSAADIDWQDLAGVASVGLTAGAS
                     APEILVEEVIEAFRARYQINVTTLSLTNENVSFRVPPIPERRLRTAS"
     gene            13129..14346
                     /locus_tag="P5558_04780"
     CDS             13129..14346
                     /locus_tag="P5558_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRY23655.1"
                     /translation="MASRTPVALADGTKIEEWRLLLILVVSTGIGPFSMQIFLPALPA
                     IQSGFAVSAATAQLAFSLSALAMAVATLIYGPLSDRLGRRPALIAGLVIFLAGSLICA
                     FANSIELLILGRVVQAAGGCAGMVLGRAIVRDLYDRERSAAAIAYITMAMVVAPMLAP
                     AIGGVLTDLFDWPAVFLLGGALGVLVLIAVRGHLPETLVPRDRPTTFSVMLGDFKTLI
                     RLPAFLGFAFQGGFSMAVFFSFLAAAPYVMITVLERPPSEYGLMFIVVSAAFMAGNFT
                     TARLGRRLGIDRMVLGGSAGTLFGACVLLLLVPAVYITPWALFLPMMLIAFTQGVSMP
                     NSLAAVISLRPDLAGAASGLAGFLQMGTAALVAQIIGSIQSGSPYPMAIGMLVSAALM
                     MLSAVYAIGRTRG"
     gene            14425..14853
                     /locus_tag="P5558_04785"
     CDS             14425..14853
                     /locus_tag="P5558_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY23656.1"
                     /translation="MASADDEYVLEQQVGHLLRRANQRHTALFARHFAAFDLTPLQFA
                     VLMKLQAAGPLSQNHLGRLTAMDPNTAQGVILRLLRRRLVMRQPSPEDRRRKLLSLTE
                     AGAALASRLKEEGRAISEATLAPLTARERRQLLALLARLV"
     gene            15000..16853
                     /locus_tag="P5558_04790"
     CDS             15000..16853
                     /locus_tag="P5558_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRY23657.1"
                     /translation="MNEISHLVGAAGPDSLVVRFAGDSGDGMQLTGSRFTQEAALDGA
                     DLATFPDFPAEIRAPVGTTFGVSAFQIQFGGRRVRTPGDRLDVLVALNPAALKVNLED
                     LRPGGTLIIDTAAFVDRNFKKAGYDHDPRDDLDGYRRLDIDITKLTQAAVKPLGVGNR
                     DANRARNLWTLGLVLWLTGRSRGATQDWIRERFAAEPLVRDANLAALDAGHAYGETAE
                     LPRLPEVLAPGQTAPLPPGRYRTVTGVDALCYGLVTAARLSGIDLFYGSYPITPASSM
                     LHMLARLGAFGVRTFQAEDEIAAICVAIGASYAGQLGVTGSSGPGIALKMEALGLAIA
                     TELPLVVIDAQRGGPSTGLPTKTEQSDLFQAVLGRNADAPLVVLAASTPGDCFPVAIE
                     AVRLATRYMTPVMLLTDGFLANASEPWLIPDLATLEPFPVRQGAAVDGFQPYARDPAT
                     LARAWVPPGTPGLRHRIGGIERSHRTGAISYDPANHQMMTDFRRAKILGIARDLPAQG
                     FVAGGMDDRILILGWGSTFGAIESAVDRLRADGVSVAHAHLRHIWPLPANLGDILASF
                     ERVLVPELNTGQLHLLLQGVYGCRLESLTKVSGKPFTAAEIETRVKTLLEA"
     gene            16858..17871
                     /locus_tag="P5558_04795"
     CDS             16858..17871
                     /locus_tag="P5558_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRY23658.1"
                     /translation="MATVLEREPKGLTPKDWASDQEVRWCPGCGDYAILKAVQKTLAD
                     LQADPDRTVFISGIGCAARFPYYVESYGFHTIHGRAAAIATGVKLANPELDIWVVSGD
                     GDSLSIGGNHLLHLLRRNVDLVYLLFNNEVYGLTKGQASPTSPRGTVSPSSPGGTLDA
                     PVNAGAFALGAGARFVARVADVVQGPMVEVLTRAHGHRGTSFVEILQNCIVYNDGAFD
                     AVTDKATAAEHQLWCRHGEPLLFAGGTKGVRLVPERFALEIFDVGADGLGAEAALVHD
                     ETNPMLAQMLVRLDVPTALGVIFAVEAPSYDAAFSAAAPTRKPGLADLQRQVGGRTTW
                     KIG"
     gene            17988..18863
                     /locus_tag="P5558_04800"
     CDS             17988..18863
                     /locus_tag="P5558_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY23659.1"
                     /translation="MGAYHRPEDLDRVLRLLAARAVAPLAGGTDLFPADAARNAWGEP
                     ALDTPEGADLLDLSGLSVLRRIEDTGDRVEIGALVTWTDALDAPLPPWFDAVRQAARE
                     VGGMQIQNRGTLAGNLCNASPAADGVPPLLALDARVRLASVRGGRELPLDRFILGNRR
                     TARAPDELVTHIVLPKPPAAARSVFLKLGARRYLVISIAMVAVTFVLDDEGRIALLRL
                     AVGACSPAAVRLCELEERLLGLTPEAAAAAVGPADLAMLTPIDDIRASGAYRRHAALV
                     LLRRALGGERLQVAA"
     gene            18860..21598
                     /locus_tag="P5558_04805"
     CDS             18860..21598
                     /locus_tag="P5558_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY23660.1"
                     /translation="MNALSPVEVGIGFRLNGRPVVIDAPPATRLSRVLRENLGLTGTK
                     VGCDAGDCGACTVLIDGRQACACLLPLGQVGGRSVTSIEGLAAEPRFSRLQQAFVEGG
                     AVQCGICTPGMLMAAADLLNRCERPGRGEIEDALGGVLCRCTGYRQIVAAVAEAIAPR
                     APAPRPAGPVVGARIARLDGRDRAAGTERFGADGVPADTWRLRVVRSPHMRARFSLGD
                     LAAVERQHGVRVLTAADVPGVNGFAIFPDRKDQPVFAVDHVRFRGEAVLALVGPAAAL
                     AGIRDAGLPITWEPLEPVSFAAARTGEAPALHGDRPDNVLTEGRLEPGDAAAALAAAA
                     VTAEIAIETSFVEHAYIEPEAGWAVRRGDRLELTVSTQSPYMDRDEVANVLGVAPEQV
                     RIRPTACGGGFGGKLDVAVQPLLAVAAWRLDRPVACTYSRAESMASTTKRHPAAIVAR
                     AGADASGRITALDFAGDFDTGAYASWGPTVAGRVPIHSPGPYRVANLRARSRAILTNA
                     PPSGAFRGFGVPQTLLAVEQAYDELALKLQIDPLEFRLRNALRPGDATATGQMLESVG
                     MVECLEALRPRWRELRAAAKGSGPVRRGVGIASVWYGCGNTGMSNPSSQRVSLLRDGR
                     IMFWNGVQDIGQGSSTIMAQILADAVGVPLDAIDSSSADTDLTLDCGKTSASRQTFVS
                     GRATQLAGEALRAQLLRLANAGGDARIEPGAGSVRVVEGGHERRIDLALLPYVEGCGP
                     EVALDARASFDPPATPVLDNRGSPYASYGFGAQIAQVAVDIELGTVKVERIVAAYDVG
                     RAINPELVEGQIIGGVAQGLGLALMEEFIPGRTENLHDYLIATVGDMPEVEVHLVEKA
                     EPLGPFGAKGVGEHALIPTAPAIMNAIHHATGVRITKIPAIPHRVHAALVAAGVSP"
     gene            21604..>22970
                     /locus_tag="P5558_04810"
     CDS             21604..>22970
                     /locus_tag="P5558_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HRY23661.1"
                     /translation="MTERFGAREADGIVRCDACPVLCRIRPGRSGACGRYANEDGSLV
                     RTDPVLFAERHVDEGGALVPFAEGDRDGAVGPSVETFVTGIGSGTTYPDYKPAPFIVS
                     STYRGVDTVTVVSEGIFSYCGCKVKIDTDRHLGAERSAITVKGEPVGHVTTAEYGSQM
                     LSIGGVHHLTGTTKKAGNVTCDTLVRLCGGEPVELEIEAGSSVVVQAGAAPIVNGKPE
                     ARMRVGCGSATVGIFAKQWYGHVDEVIVLDDHITGVLTEHQAGRCLDMPPSGVRIEGR
                     KSTPGRYFQVANPGLGWGGTDITDPFSVIRDIDRKLAWPGMRLLITSTTGEDAVWCVL
                     DEDFRLREAEMPAAVRRTVERIGENCEPSLCSVLFMAGAGGSLRAGVSENPVLITRSV
                     KAGETRVTMGGAPVYVWPGGGITVMVDVERLPKRAFGYVPTPALVAPIEFTLPLELYT
                     ALGGHA"
     assembly_gap    22971..23070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23231..24106
                     /locus_tag="P5558_04815"
     CDS             23231..24106
                     /locus_tag="P5558_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HRY23662.1"
                     /translation="MTARAMAWGGPAAARLAGNRLHLAHGPIDLVIRADGPSASVERA
                     YRAAGAAFAGLLESLVAVLPRLRAPVGSAAGLPPAARLMQAATAPLATDLFVTPMAAV
                     AGAVADTVMTAIRAVPGIERAYVNNRGDIAVHLTPSASIEIGVVPELRRPLMAASLRL
                     RHDDGIGGVATSGWDGRSFSLGIADAVTVLAASAAQADAAATLIANAVDVDDLTITRT
                     PATELDPDSDLGRLLVTTGVGVLDDAAIAAALDNGARYAQTLLERRLISAAMLVLEGR
                     ARIVGRIRGEIACSS"
     gene            24094..24678
                     /locus_tag="P5558_04820"
     CDS             24094..24678
                     /locus_tag="P5558_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HRY23663.1"
                     /translation="MFELRKTQVTVEEIHHDGGPHLDRPLLRGAVAAVFKNPFAGRFA
                     DAGEFVEAMDALKPLGLEMSRKLIQALGGDPRRIESYGKGSIVGSDGELEQGALWHVP
                     GGYAMRELLGDARAIVPSSTKLGGMGAALDVPLHHINAAYVRSHFSAIEVRVADAPRP
                     GEIVFCLAMTTGGRPHARMGGLEAADIKGQDGLR"
     gene            24708..>24913
                     /locus_tag="P5558_04825"
     CDS             24708..>24913
                     /locus_tag="P5558_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRY23664.1"
                     /translation="MAFVRNNWYPAGFASRMGEGLHKRRMLGEDLVIWQQADGSFAAL
                     QDRCPHRFLPLSMGCLERDTIRCG"
BASE COUNT         3625 a         8457 c         8823 g         3908 t
ORIGIN      
        1 ggtccgggtc gagcgcctga taccacgcga cgagtcggcg ccacgctcgg ctgccgatgt
       61 cattcccgat gaattgcagg ccgagccggt cccgcccgag accgccgagg acgacagcct
      121 cgacgcattg ctcgccgatc tggccgaggg ccccaaggag ctgcgtgaga tcgagcccgg
      181 cgatggtggc gctgtcgcga gcgtgccaga ggaccagcaa attccgctgg ttccggcagc
      241 cccgccagtt ccagccgctg atcctgtctc tccgcagccc gtcgccgagg ccccgaggcc
      301 gatcgaggag ccgacaccgt cagcgccggc accgccggca cctgaagcga gcgcgccgac
      361 tgttgcggct tcggcgccgg agcccacggc ggcaacccgg gcacctgccc cacgtacggc
      421 gccggcaccg gccgcagccc cggcgcgaac cgcgaccacg acaccggcaa ggccggcggc
      481 accgccgacg ccacgcacac aggtggcggc agcaccagcc gcatcgggcg agctgacggc
      541 gagctaccgt ctgcaattgc tggcggtgcg cgacgaggcg gctgccgcgg cggcctggtc
      601 ggatctgcaa cggcagcacc cgtccgtgct cggtggcctg cgcagccggg tcgagcaggc
      661 caatgtcggt ggcaacacgt tctttcgcct gcaggccgga ccgtttcagg gtcgcgatgc
      721 ggccgaccgg gcatgccgtg tcctgcagca ggcgggcagc gactgcttca tcgtcgggcc
      781 gacttcttga gggcgtcggc ggggacgagc caggccaggg ccgccatcgt cggaattgcc
      841 ggaaccgccc tcgagcccgc ggagcggcga ttgctcgcca gaaatccacc ggccggtttc
      901 atcctgttcc agcgcaattg cgagagccgg tcgcaattgc tggccctgac caacgagctg
      961 cgggcgctgt tccccgatcg ctgggtgccg atcctcgtcg accaggaggg cggccgggtg
     1021 cagcggctcg gccccccgca ttggccacag ctctggccgg cgcgcatgct gggtcggctg
     1081 gcggagcgcg atcgggcggc gggcgaggcg gccgcggcgt tgcaggccag ggcgatggcg
     1141 acgatcctgc gcgaggtggg catcgacgtc gtctgcgcgc cgtgcctcga tctcctgatg
     1201 ccggagacga ccggggcaat cggcgatcgg gccttcgccg ccgacccggc gctggtcgcc
     1261 gggctcggcc gcatcaccgc cgatacactg atggcttcgg gcgtcctgcc gatcatcaag
     1321 catcttcccg ggcacgggcg ggcgacgtgc gacagccatc tcgagctgcc gcgggtcgtg
     1381 gccgaatggg ccatcctcga cgccagcgac tggcaggcct tccggccact ggccgatctg
     1441 ccgctcggca tgaccgcgca catcgttttc gccgcgatcg atccggaccg cccggcgacc
     1501 cagtcgccca gggtgatcgc ggaggtcatc cggggtgcga tcggctttca ggggctgctg
     1561 ctgagcgatg atctgagcat gcaggcgctg tccggaccaa tcgaggaacg ggccagcctc
     1621 gccctagcgg caggctgcga cctggccctg cactgcagtg gtgacgcggc cgaggtcgac
     1681 gccgtcctcc ggaccgtttc cgacctcgag catgccagca tgacgcgcct gcagtcgttg
     1741 cgtcccgggc cagccgccgg cgaggccctc gacgatctcg tgaccaccct cgccggcctg
     1801 ctcgaagccg aggtcggtcg tgcctgacct cgtcgctctc ggccacacgc tctcggtctg
     1861 gtttctgccc gtggtgatcg cgatcaccat gcacgaggct gcgcatgcct gggccgccga
     1921 caagctcggc gacgacacgg cgaagtcgct cggccggctc agcttcaacc cgatccggca
     1981 cgtcgatccc ttcggcacgc tggtgctgcc cggcctgctg ctgctcgtcg gcgcgccctt
     2041 ccttttcggc tgggccaagc cggtgccggt cgccttccat cgcctgcaca acccgaagcg
     2101 ggacatgata tgggtggcgc tggccggccc gggcatcaat atggtgatgg ccctggcggt
     2161 ggcgttgctg atgcatggct tcgtcgggac gcaaagcgag atcggccaat gggtctggca
     2221 gaatctcgac aacgcgctgc tcatcaatgt cgtgctcgcc tgcttcaaca tgctgccgct
     2281 cctgccgctc gatggcggtc gggtgctgac cgggcttctg ccggtcccgc tggccgtcaa
     2341 gttcgcccgc accgagcgct acggtctgtt catcctgctc ggtgtcgtct tcgtgctgcc
     2401 gctgctcgcg cgcgagctgg gctttgccat caaccccctg gcggccatcc tcctgccgat
     2461 ggtgcagacc gttcattcgc tggtcctgct gctcaccgga tggagctgat gagcgccctg
     2521 tcgcccgatc gattcgtcgt cgacctcgac gggttcgagg ggccgctcga tctgctgctc
     2581 gagctcgccc gcgcgcagaa ggtcgatctg tcgcgcatct cgatcctggc gctggccgat
     2641 cagtttctcg cctatctcga ggaggccagg gcccgcgagc tgtcgctggc ggccgagtat
     2701 ctggtgatgg cggcctggct cgccttcatg aaatcgcagc tcctcctgcc cagggaggca
     2761 cgcgaggacg aggacgtcga cgcgatggcc gaggcgctgg ccgcccggct gcgcaagcta
     2821 gatgccatcc gccgggccgt cgcatggctc gatctctgcc cggagctgga ggtggcgcga
     2881 tatgcgcgcg gcgctcccga atccgccccg gttcgccacg aggaccactg gcgcgccgat
     2941 ctggccggtc tcatggagtc ctatgcccgg ttgatggcgc gacggcggca gcaatccatc
     3001 cggctggtgc cgcgtcgggc gatgagtgtc gaggcggcgc tcgagcggct gtcgcggctc
     3061 ctgaccggcc gggaatggca ggatctcgcc ggcttcctgc cacccgggct cgccgacgtg
     3121 tttctcgggc gctcggcgct ggcggcgtcg ctggtcgcca gcctggagct ggccaagcgc
     3181 ggcaccatcg agatcgacca ggcggcaccg ttcggcccga tccggatccg gcggcgatga
     3241 gcgacgaagg cgaggcgatc gacgccaagc tggtggtcga ggcgctgctc ttccagagtc
     3301 gcgagcccct ggacgaggcg ctgctgcagg cgcatgtcag ggacggaagg catgtgctgg
     3361 ccattctcga ggagctcgcc gcggattatg cagcgcgcgg cgtccggctc gagcggcacg
     3421 gtcgccagtg ggcgttccgc accgcagcca gcgtggcacc ccatctggag cgcgtcgagt
     3481 cgcgtcgtcg caggttgtcg aaggcggcga tggagacgct tgcgatcatc gcctaccagc
     3541 agccggtgac ccgtgccgag atcgaggcgg tgcgcggggt gccggttgcc cggggcacgc
     3601 tcgaccagct cgtcgaggcg ggctgggtca gacccaaggg gcggcgcgag gtgccgggcc
     3661 ggccggtcac ctgggtgacc acgccgggct ttctcgatca attcgatctg agctcgctcg
     3721 acgatctgcc acgcctcgac gagctggagg ccgccggcct gttccggacg gccggaggtg
     3781 gtggatcggc gcccgatgac gcgacggcgg aggcccacgg tcaggccgat atcgagctgc
     3841 ccccgggagt ggccgggacc tgattgcaca gccccagttt ttttgccaga ctgctgaaac
     3901 atccgcagaa cgcgccttcc cggctccggg agttgggctg cggcatcaag agggttcccc
     3961 atgagtatcg gtatctggca ggtcgtcctg atccttgtga tcgtcctcat cctgttcggc
     4021 gcgggcaagc tgccgaaggt gatgggcgac gtcgcatcgg gcgtgaagaa cttcaagaag
     4081 ggcatgaagg acgaggacga ggagcagacg ccgcgcgcgc tgagcgagga ggcgaccagc
     4141 cccgagatga agatggatcg gaccaaggac gaggcggcca agagctaggt gcggcggcga
     4201 gcggcacggc agcggctgaa gggcgcgcat gttcgatctc ggctggtcgg aaatggccgt
     4261 tatcatgctg gtggcgctta tcgtcatcgg cccgaaggat ctgcccaagg tggccaagac
     4321 gattggccac tggacggggc gggcgcgtgc gctggcacgc gatttccagc gctcgatcga
     4381 cgacatggcg cgcgaggcgg agctcgacga gatcaagaag agcatcgacc ggaccggcag
     4441 gatcgatctg cgcaagacca tcagtgacgc cgtcgatccg aagggtgagc tcgcgcgcgc
     4501 cttcgacgtc gaggacaagg cgaagcccgg caaagtcggc aagacggcgc ctgccaagcc
     4561 gaggatcgag gcgccggcgg caccggccgg gccgcccgcg gccgtcgccg acgacaccgc
     4621 gacgtccgag ccggacaagg atgcggcaac cctctcggac ggggcgacca cgccccgctc
     4681 ctagcgcgac gaacggacga accctgccgt gatcaaggac atcgacgacc gcgaaatgcc
     4741 gctgctcgac catctgctcg agctgcgcaa ccgcctcatg tacagcgccg cggcgatcct
     4801 gctcggcttt gtcgtctgct acttcttctc cgagcagatc tacgccttcc tcgtccgccc
     4861 gctcgctgcc gtctacgagg gccagaccgg ccggcggatg atctatacga gcctgaccga
     4921 ggcgttcttc acctatctca aggtcgcctt cttcgccggc gccttcatca ccttcccgtt
     4981 cgtcgcgacc cagctctacc ttttcatcgc gcccgggttg tatcgaaacg agaagcaggc
     5041 cttcctgccg ttcctgatag cgacgccggt gctgttcttc ctcggcgggg cgatggtgta
     5101 ctacgtcatc ttcccgctgg cctggcgctt cttcgtgagc ttcgagaccg ccggtggcgc
     5161 cgggaccctg ccgatcgagc tggaggcgcg cgtcggcgaa tatctcgacc tcgtgatgaa
     5221 gctcatcttc gccttcggcc tctgcttcca gctgccggtg gcgctgaccc tcatggggcg
     5281 ggtcggcctg gtcactgccg acggcctcgc ccgcaaccgt aaatatgctg tcgtcggcgt
     5341 cttcgtcgtc gccgccctgc tcacgccgcc ggacatcatc agccagctcg gcttggctct
     5401 gccgatcctg gcgctctacg agatctccat ccatctcgtg cgactggccg agaagaagcg
     5461 ggacgaggcg caggccgccg aggaagctgc cgccgacgac gattgaggcg cgggggcggc
     5521 ggtcatggcg aatggcgctg gaaatgtcac ccgggaaagg tgatggcgac ccctacggga
     5581 atcgaacccg tgttttcagc gtgagaggct gacgtcctga ccgctagacg aaggggtcgc
     5641 gagcgatggc tgctaggtag aagctcgggc gacgcggatc aagccccttt cggttgccgc
     5701 gtgaagaacg cggcgagctc gacctcggcc gtgtagacaa actggtcgac gatctcgagc
     5761 gccgcgagcc gatagccgcc ggcgacgagg acagcggcat cgcgggcgaa gctcgccggc
     5821 gcgcaggaga catagacgat ccggtccacg cccgacctcg cgagctcggg cacctgcgcc
     5881 tcggcgccgg cgcgtggcgg gtcgaggatg atggcgtcgc agccggcgag ctcgctcggg
     5941 gcgagcggtg cctggaagag gttgcgacag ctcgcctcga tgcgtggatg gcggcaggcg
     6001 gcgacgagct cggccgcgac gtcgaaggcc cggatcctga cattcggccc ggccagcggc
     6061 aggctgagcg tgcccacgcc ggcgaacagg tcgacgacgc ggcgggcccc ttcgagatgg
     6121 cgcacgacga aggcctgcag gcaggcctcg ccttccgccg tggcctgcag gaaggccgcg
     6181 ggcggcaagg agaccggtag gccggcgatc gtgagagcgg caggcaggcg ggcggccacg
     6241 ggctccggct cggcaccgtc gacggtgatg gcgaggcgca ccagggcctc tgcctcggcg
     6301 aacgccgcga gcgtctcgcg ctcggcgagg ctcggctgca cggccagatg gagcacgact
     6361 tcgacacccg aatcgagcgc tgtcagcgtc acctcgccgc ccttcctggc gcagtcgagc
     6421 cggggcagga gctgccgcag gggagcgaag agggcgacca gcccgggccg cgcgatcggg
     6481 cagctctcga tcgccacgat ggcgctgctg tggcgctggc ggaagccgag ccggccgccc
     6541 ttgtcgaagc cgagccgtag ccggcggcga ctgccgagcg ggctgcggtg gatgacgatc
     6601 ggttcgggga cctcgacgtg ccgggcgcgc agcgcgcccc ggacgtggcg tcgtttcaac
     6661 tcggcatagg cctcgggccg gagatgctgg agcgtgcagc cgccgcacat gccgaaatgc
     6721 ccacaagggg gccgcgcccg ctcggtgctg gcgacgaggc aggccaccgg atcgagccag
     6781 ccccggcgca cgccgcggcg cacctgccag cgctcgcccg gcagcgctcc aggcaccagc
     6841 agccgctcgc cgtcgaggat ggccacgccg tcgcccttga atccgagctc ctgaatggcg
     6901 atctcaaagg gatgcgccga tcgttgctgg cgccgtctca agggcgtgct gtctcgacga
     6961 cgaagctggc gctcgacgcc gggtcacgtg gatcgagacg caacagcagc aaagcgtcat
     7021 cgtcgtcacc gagcagcaga aagacgaagt cgctggtggc gctcatctcg atcacgcgaa
     7081 agccggttgg caggacgatg ctgccgctgt cgacggcggc ccggtcgcct cggccgcgct
     7141 cgaccatcag ccagaccagg gcgacgacgc cgagcacgat caccacggcc gccacgatgt
     7201 tgaagagctt gatgatgccg agataccgtt ccatgacctg tccgccgctg gctgctgccc
     7261 gcttgccaca tggctgagcc ggcgctggtc gagattactg acggcgaggc cgggcgccgg
     7321 gtcgatgcgg tgctggccga gcggttggac gacttgtcgc gttcccggct gcaggcgttg
     7381 ctgcgcgacg ggcacctgac cgtcggcgac gcggtggtaa ccgagggcaa gcggcgggtc
     7441 aaaccgggtg aatgctaccg cctggtcgtg ccgccgccgt cgccttcggg gctcgtcgcc
     7501 gaggatcggc cgctcgacgt ggtcttcgag gatgacgatc tcctggtcgt cgacaagccg
     7561 gccgggctcg tcgtccatcc gtctgtcggg cacgatcatg gcactctggt gcacgcgctg
     7621 ctggcgcatt gcggcgcgag cctctcgggc atcggcgggg tggagcgacc ggggatcgtc
     7681 catcggatcg acaaggacgt cagcggcctg ctcgtcgtcg ccaagcacga tcgggtgcat
     7741 cagggccttg ccggccagtt caccgtgcac agcgtcgatc gactctacga ggcggtggtc
     7801 cacggcctgc ccagcccggc cgccggcacg atcgaccagc cgatcggccg tcacccggtc
     7861 gaccgcaagc ggatggcgat cgtgccggcc ggcaagcggg cggtgacccg ttaccgcctg
     7921 ctggaggcag ggggcacgtt gatcgcccgc ctcgaggtgg cgctcgagac gggccggaca
     7981 caccagatcc gggtgcatct ctcgagtctc ggtcatccgc tgctcggtga ccggctctat
     8041 cggtcgcgcc gtcgccagga gttgccggca ccgctgcggg aagagatcga gggcctgggt
     8101 cgggtggcgc tccatgcccg cgtgctcggc ttcacgcatc cacggacagg cgagcggttg
     8161 cgcttcgagt ccccgccgcc ggccatattc gatcgactcc tagccctcgc tcgggcatag
     8221 tttcaggggg tgtggcattc ggactgtgct cgccgaattt tcatgcaacg aatcagtcct
     8281 gtcgatgtta agggttcggg tgatcgcgta gcagccggga accgatagcc ggcacgtgat
     8341 ccggtgagcc tgaactagcg gaacgtcatc catggccagt cttcccacag ttcccctcga
     8401 gggcagcgga ctctcgcgtt atctcgagga gatccggcgg tttccgatgc tggagccgga
     8461 gcaggaatac atgctcgcca agcggtggcg cgagaacgac gacgtcgcgg cggcgcacac
     8521 gctggtgacg agccatctgc gcctcgtcgc caagatcgcc atgggctatc gcggctacgg
     8581 cctgccgatg aacgagatca tctcggaagg caatgtcggg ctgatgcagg cggtcaagcg
     8641 gttcgagccg gagcgcggct ttcgcctcgc gacctatgcc atgtggtgga taagggcagc
     8701 catccaggaa tacatcctcc actcctggtc gctggtgaag ctcggcacca cggcggcgca
     8761 gaagaagctc ttcttcaacc tgcggcgcct caagagccag atgcaggcga tcgaggaggg
     8821 tgacctgagc cccgaggcgg tgaagaagat cgccgagacg ctggcggtca ccgagcagga
     8881 cgtcatcgac atgaaccggc gcctcgaagg gccggaccat tcgctcaacg cgaccctgcg
     8941 cgccgagagc gacatggagt ggcaggactg gctggtcgac gaccgggcaa cccaggatgc
     9001 gatgctcgcc gaggacgacg agctggatca tcgtcgtgag atgttgacga gcgccatgca
     9061 tgtgctcaac gaccgagaga agcacatcct gaccgagcgt cgcctgaagg aaagcccgac
     9121 gacgctcgag gagctgagtg gtcagtacgg catcagtcgc gagcgggtgc ggcagatcga
     9181 ggtgcgggcc ttcgagaagc tgcagaaggc cattcgcggc atggaggcga cccgcaccgc
     9241 cgaggccgcc gcggcgacca ttccgtcagg ctgaggagcg gggcgctcga tctgctcctt
     9301 tgggcccgca ggcttcaggc gtggccggcc gtcgaggaca ggagtgacgg gtcgacgccg
     9361 atcttgttga gcgcgcccgt ccatttctgg gtgtcgtcga gctcgaagac gatgtgctcg
     9421 tcgggcggcg ctatgagcca gccgttcttc tggatctcgg catcgagctg gcccgccgcc
     9481 cagccggcgt agccgagcgc gaacacccgt tgccgggggc cggtgccgct ggcgatcgcc
     9541 ttgagcacgt cgaccgtcgc ggtcagggcg aactcggcgt cgatcagcag cgtgttctcg
     9601 tgcacgtagt cctgcgtgtg cagaacgaag ccgcgactgc tttcgaccgg cccgccgaca
     9661 tggaccttga ggttcgctgc gtcggtggtg ggctcgatgc cgagctgctc gacgattgcg
     9721 gtgaactcga cgttctccgc gatctggttg actacgatgc ccatggcccc ctcgctcgag
     9781 tgggcgcaca gatagatcac cgagcgttcg aagcgcgggt cgggcatgcc cggcatggcg
     9841 acgagcagca tgccggcgag atggttgtcg ttggcgtcgt cggttcgcat gactaagaga
     9901 taacgggtcg ctgccggcgc aacaagagag ccggagccgg caatcctatc cgttgaagca
     9961 cagcaatgaa cgagagaggc ctcatgacga tcaagaccgg tgaccggctg cccgacgcga
    10021 ccttcaagcg caacaatgcc ggcgacatca cggacgtgac gacgatggag ctctgcgccg
    10081 gcaagaaggt ggtgctgttc gccgtgcccg gggcgttcac gccgacctgt cacgcgaagc
    10141 acgtgccgag ctatctgcag caccatgatg cgctgaaggc gaagggtgtc gacacgatcg
    10201 cctgcatcgc tgtcaacgat cccttcgtgc tcggcgagtg ggcgaagtcc acgggcacgg
    10261 ccgacaagat cgtcatgctg tccgacggca atgccgagtt caccagggct atcggcctcg
    10321 acttcgatgg cagcggcttc aagctcggca cgcgttccaa gcgttatgcc atggtagtcg
    10381 aggacggcgt ggtgaagacc cttgccgtcg aggaggcgcc gccgatgatg gaggtctccg
    10441 gggccgacaa gatcctggca gcgctctgag ccgcctcagc gctcggcttt cgctgccctg
    10501 atcgccgccg tggccagacc gtcggcccgc tcgttgagcg ggtcgccggc atggcccctg
    10561 gtccatgccc actcgatctc gtgccgcctg gcggcggcct cgaggcgcag ccagagatcg
    10621 acattcttga ccggcttctt gtccgccgtc ttccagccct tggctttcca gccggcgagc
    10681 cactcggtga tgcccttgcg cagataggcg ctgtcggtca cgaggcgcac gcgcatcggc
    10741 cgcttcaggc tctcgagcgc ctggatcgcg gccatcagct ccatgcggtt gttggtcgtc
    10801 gccggctcgt ggccggagag ctcgcgctcg tgatcgcgcc agcgcagcac cgcaccccag
    10861 ccgccgggtc ccggattgcc gctgcagccg ccgtcggtat ggatctcgac gacgtcggtt
    10921 gccgccggct cggtccgcag ggcgtcagtc gaggccatag gcggccggct cctcgacgcg
    10981 acggtggaag gcgagcttgc gggcgtactc catgggatcc ttcggcttga ccagggcacc
    11041 gtcgacctga ttcagccagt cgaacaggcg ggtgagcaga aaacgcgtgg cggcgccacg
    11101 gcagagcact ggcaatgcct cgatctcggc cggctcgagc gggcgcacgg cctgatagcc
    11161 ggcgagcagg gcgcgcgact tggcgagatt gaactccgcc cgaccctcga agcaccaggc
    11221 attcaggcag atggcgacat cgtaggcgaa gcggtcgctg caggcgaagt agaagtcgat
    11281 gacgccggtg acgcgatcac cggcgaaaaa aacgttgtcg gggaagagat cggcgtggat
    11341 cacccccgac ggcagcgcct gcggccagtt gcgctcgaga tcgtcgagtg cttgggcgat
    11401 ggtgtcggcg agctccgccg aaggcggggt ggggctctgc cggcaggcct cgaaaagtgg
    11461 tcgccagctc gtgaccgaga gcgcgttcgc acgatacccg gcgaagtcgc cgacggcctg
    11521 gtgaaggctg gccagagtgg cgccgagggc cgcgcagcgt gccgcgtcga tccgcttcgg
    11581 cgagcggccg tcgagaaagc tgacgatggc cgccggcttg ccgcacagtc gctgcaggct
    11641 gcggccatgg cgatcgtgga tcggcaccgg acaggcgatg ccgtgcttcg ccagatgatc
    11701 catgagcgtc aggaagaagg gcaggtcggc cgggtcgacg cgcttctcgt agatcgtcag
    11761 gatgtagcgg ccggtcgtcg tgtcgaggcg gaagttggag ttctcgacgc cttccgcgat
    11821 gggttcgagg gttctggcct cgccgatgcc gtactgcgcc aggagcgcct ccagggccgg
    11881 caccggaacc gaggtataga ccgccatcca tcaccagtct ggtcgcttgc aacccgcgcc
    11941 ttatgccgac cccggcccgg cttgtcacgc ggcacggcgg cggctcgccc gaccggtttc
    12001 aggaagcggt gcgcaggcga cgctcgggga tcggcgggac gcggaaggag acgttctcgt
    12061 tggtgaggct cagggtcgtc acattgatct ggtagcgcgc gcggaaagcc tcgatcacct
    12121 cctcgaccag gatctccggc gccgaggccc cggcggtcag cccgaccgaa gccaccccgg
    12181 ccaggtcctg ccagtcgata tcggcggcgg actggatgag cagcgcccgc gcggcgccgt
    12241 tctgcctcgc cacctcgacc agccggaccg agttcgacga gttcggtgcg ccgaccacga
    12301 gcacgagatc ggagcccggt gccatcgccg ccaccgcttc ctggcggttg gtggtggcgt
    12361 agcatatgtc ctcgctgcgc ggtccggcga tcgccgggaa ccgttggcgc agcactgtca
    12421 cgatcgcggc ggtgtcggtc agcgagagcg tcgtctgggt ggcgtaggcg accttgtcgg
    12481 gatcgggcac catcacattc tcggcgtcgg cgacggtctc gaccaagagg acggcgccgt
    12541 cgtcgacctg gcccatggtg ccttcgacct cgggatgacc ggcatggccg accaggagga
    12601 ccgtcagccc gtccgcgtgg tggcgttcga cctcacgatg gaccttgctg accagtgggc
    12661 aggtggcatc cgcgaaaatc agccggcggc ggcgcgcctc cgccggcacc cgcttcggca
    12721 ccccgtgggc ggagaagacc accacggcat cctccggcac ctcgtcgagc tcgtcgacaa
    12781 acaccgcgcc gaggccacgc agctcctcga cgacatggcg gttgtgcacg atctcgtgac
    12841 ggacgtagat aggcgggccg ttcttccgca acgcctcctt gacgatggcg atggcccggt
    12901 ccaccccggc gcagaagccg cgcggattgg cgaggcagat ggtcaaatgc ggtcggtcgg
    12961 ttggcggcaa gacgagctcc ggcgcggtgt gcggccggcg atcgggtcca gggccggccg
    13021 gctgacgaac ccgtcggtcg atagcatagc gggccattgc cggcgagagc cgatggcaca
    13081 ggtctggcgt gcggcatcgg cgctgcgcga gcacgggaga ggacgaggat ggcgagccgc
    13141 acgccagtgg cgctggctga cgggaccaag atcgaggagt ggcggctgct cctcatcctg
    13201 gtcgtgtcca ccggcatcgg cccgttctcc atgcagatct tcctgccggc cctgccggcg
    13261 atccagtcgg ggttcgccgt ctccgccgcc acagcgcagc tcgcgttctc gctttccgcc
    13321 ctggcaatgg ccgtggcgac gctgatctat gggccgctct cggaccggct cggccgccgg
    13381 ccggccttga tcgccggcct cgtcatcttc ctggcaggca gcctgatctg cgccttcgcg
    13441 aactcgatcg agctgctgat cctcggccgt gtcgtgcagg ccgccggggg ctgcgccggc
    13501 atggtgctcg gtcgcgcgat tgtccgcgat ctctacgatc gcgagcggtc ggccgcggcc
    13561 atcgcctata tcaccatggc aatggtggtg gcgccgatgc tcgccccggc gatcggtggc
    13621 gtgctcaccg acttgttcga ttggccggcc gtcttcctgc tcggcggagc gctcggcgtg
    13681 ctggtgctga tcgccgtgcg tggccacctg cccgagacgc tggtgccgcg cgaccgaccg
    13741 accacgttca gcgtgatgct cggggatttc aaaacgctga tccggttgcc ggcattcctc
    13801 ggcttcgcgt tccagggtgg cttctcgatg gcggtcttct tcagcttcct cgccgcggcc
    13861 ccctacgtga tgatcaccgt gctcgagcgt ccgccgtcgg aatacgggct gatgttcatc
    13921 gtcgtctcgg cggccttcat ggccggcaat ttcacgaccg cccggctcgg ccgccggctg
    13981 ggcatcgatc gcatggtcct cggcggcagc gccgggacgc tgttcggggc atgcgtgctg
    14041 ctcctgctgg tgccggccgt ctacatcacg ccttgggcgc tcttcctgcc gatgatgctg
    14101 atcgccttca cccagggcgt gtcgatgccc aacagtctcg cggcggtgat cagcctccgg
    14161 ccggatctgg ccggtgccgc ctccggtctc gccggcttcc tgcagatggg cacggccgcc
    14221 ctggtggccc agatcatcgg ctccatccag agcggctcgc cctatcccat ggccatcggc
    14281 atgctggtca gcgccgccct gatgatgctg agcgccgtct acgcgatcgg tcgcacgcgc
    14341 ggctgaagcg aggcgccatt cgtcggccct tgcagccggt ccgggcgaca ggcacaagcg
    14401 ccggacgaca gcaacgggcg acgcatggca agcgcggacg acgagtatgt gctcgagcag
    14461 caggtcgggc atctcctgcg gcgggccaat cagcgccata cggcgctgtt cgcccgtcat
    14521 ttcgccgcct tcgacctgac gccgctgcag ttcgccgtgc tgatgaagct gcaggctgcc
    14581 ggccccttgt cgcagaatca tctcggccgg ctcaccgcca tggaccccaa caccgcccag
    14641 ggcgtcatcc tgcgtctcct gcggcgccgg ctggtgatgc gccagcccag cccggaggac
    14701 cggcggcgca agctcttgtc gctgaccgag gcgggcgctg ccctggcgag tcgactgaaa
    14761 gaggaggggc gggcgatcag cgaggcgacc ctcgcaccgc tgaccgcgcg ggaacgtcgc
    14821 cagttgctgg cgctgctggc gcggctcgtt tgagtaggag gccgtcccgg atcggcttga
    14881 cagttgcgtg agctggggcc atcgccatgc gcaatcggct tgcgacggct cctgcagggg
    14941 ctattgtagg tgttcttacg aattgccggg cggtgggacc gctcgctagg gggcttgcga
    15001 tgaacgagat cagccatctc gtcggagccg cggggccgga ttccctggtc gtccgcttcg
    15061 ccggcgacag tggcgacggc atgcagctca ccggcagccg gttcacccag gaagcggcgc
    15121 tcgacggtgc cgatctcgcc acgtttccgg attttccggc ggagatccgt gccccggtcg
    15181 gcacgacctt cggggtctcc gccttccaga tccagttcgg cggccggcgg gtgcgcacgc
    15241 ccggcgaccg gctcgatgtg ctcgtcgctc tcaatcccgc ggccctgaag gtcaatctcg
    15301 aggatctgcg gccgggcggc acgctgatca tcgacacggc ggctttcgtc gatcgcaact
    15361 tcaagaaggc gggctacgac cacgacccgc gcgacgacct cgacggctat cgccgcctcg
    15421 atatcgacat caccaagctg acccaggccg ccgtcaagcc gcttggcgtc ggcaaccgcg
    15481 acgccaaccg ggcgcgcaat ctctggaccc tcggcctcgt cctctggctc accggccgct
    15541 cgcgaggcgc cacgcaggac tggattcgcg agcgcttcgc cgccgagccc ctcgtccgcg
    15601 acgcgaatct ggcggcgctc gatgcgggcc acgcctatgg cgagacggcg gagctgccgc
    15661 gcctgccgga agtgctggca cccggacaga cggcaccact gccgcccggc cgctaccgga
    15721 cggtgaccgg agtcgacgcc ctctgctacg ggctggtcac ggcggcacgc ctctccggca
    15781 tcgacctctt ctacggctcc tatccgatca ccccggcctc gagcatgctg cacatgttgg
    15841 cacggctcgg cgccttcggt gtgcgcacct tccaggccga ggacgagatc gccgccatct
    15901 gcgtcgccat cggtgcttcc tatgccggcc agctcggcgt caccggcagc tccgggcctg
    15961 gcattgcgct caagatggag gcgttgggcc tcgccatcgc caccgagctg ccgctcgtcg
    16021 tgatcgacgc gcagcgcggc ggaccctcga ccggtctgcc gaccaagacc gagcagtcgg
    16081 acctcttcca ggccgtgctc ggccgcaatg ccgacgcccc gctcgtcgtc ctggcggctt
    16141 cgacgccggg cgactgcttt ccggtggcga tcgaggcggt gcggctcgcc acgcgataca
    16201 tgacaccggt catgctgctg accgacggct tcctcgccaa tgccagcgag ccctggctga
    16261 tcccggatct ggcgacgctc gagccctttc cggtgcggca gggtgctgcg gtggacggat
    16321 tccagcccta cgcccgcgat ccggcgacgc tggcgcgggc ctgggtgccg cccgggacgc
    16381 cgggcctgcg ccatcggatc ggcggcatcg agcggtcgca caggacgggt gccatttcct
    16441 acgatccggc caaccaccag atgatgacgg atttccgtcg ggccaagatc ctgggcatcg
    16501 cccgggatct gccggcgcag ggctttgtcg ccggcggcat ggacgaccgg attctgattc
    16561 tgggctgggg gtcgaccttc ggcgccatcg aaagcgccgt ggatcggctg cgggccgacg
    16621 gggtcagcgt ggcgcacgcc catctccgcc atatctggcc gctgccggcc aatctcggcg
    16681 acatcctcgc gagcttcgag cgggtgctgg tgccggagct gaacaccgga cagctccacc
    16741 tcctcctgca gggggtctat ggctgccggc tggagagcct gaccaaggtc tccggcaagc
    16801 cgttcaccgc cgccgagatc gaaacccggg tcaagacgct gctggaggcg tgatacgatg
    16861 gcgaccgtgc tcgaacgtga gcccaaggga ctcacgccca aggactgggc cagcgaccag
    16921 gaggtccgct ggtgccccgg ctgcggcgat tacgccattc tcaaggcggt gcagaagacc
    16981 ctggccgacc tgcaggccga cccggatcgc accgtgttca tctcgggcat cggctgcgcc
    17041 gcccgttttc cctattatgt cgagtcctac ggtttccaca cgatccatgg ccgggcggcg
    17101 gcgatcgcca cgggcgtcaa gctggccaac ccggagctcg acatctgggt cgtctcgggc
    17161 gacggcgaca gcctgtcgat cggtggcaac cacctcctcc atctgctgcg ccgcaatgtc
    17221 gatctcgtct atctgctgtt caacaacgag gtctacggcc tgaccaaggg gcaggcctcg
    17281 ccgaccagcc cgcgcggcac ggtgagcccg tcctcgcccg gcggcacgct cgacgcgccg
    17341 gtcaatgccg gggccttcgc gctcggcgcc ggggcgcgct tcgtggcgcg ggtcgccgac
    17401 gtggtgcagg ggccgatggt cgaggtgctg acccgggcgc acggccatcg cgggacgagc
    17461 ttcgtcgaga tcctgcagaa ctgcatcgtc tacaacgacg gcgccttcga cgcagtgacc
    17521 gacaaggcga ccgcggccga gcaccagctc tggtgccggc acggcgagcc gctcctcttc
    17581 gcgggcggca ccaagggcgt gcgcctggtg cccgagcgct tcgcgctcga gatcttcgat
    17641 gtcggcgcgg acgggctcgg tgccgaggcg gccctcgtcc acgacgagac caacccgatg
    17701 ctggcgcaga tgctggtgcg gctcgatgtg ccgaccgcac tcggcgtcat cttcgccgtc
    17761 gaggcgccga gctacgatgc ggccttcagc gccgcggccc cgacgcgcaa gccggggctc
    17821 gcggatctgc agcgccaggt gggcggccgg acgacctgga agatcggtta ggcgggccgg
    17881 gccgcaagcg cgcttgacag ggcggccgtg cggtccgagt ttgttccatc acacatgaat
    17941 tcgggcgctg acccgagccg tgggagaggg tagccgagcc agcgcgtatg ggagcctatc
    18001 accgccccga ggatctcgac agggtgcttc gcctcttggc ggcgagggcg gtcgcgccgc
    18061 tcgccggcgg caccgatctg ttccccgccg acgcggcgcg caacgcctgg ggcgagccgg
    18121 cgctcgacac acccgaaggt gcggacctgc tcgatctgtc cggcctgagt gtgctgcgcc
    18181 gcatcgagga cactggcgac agggtcgaga tcggcgccct cgtcacctgg accgatgccc
    18241 tggacgcgcc gttgccgccc tggttcgatg ccgtccgcca ggcagcacgc gaggtgggcg
    18301 gcatgcagat ccagaatcgc ggcacgctcg ccggcaatct ctgcaatgcc tcgcccgcgg
    18361 ccgacggcgt gccgccgctt ctggcgctcg atgcccgcgt ccgcttggcg agcgtgcggg
    18421 gcggccgcga gctgccgctc gacaggttca tcctcggcaa ccggcgcacg gcaagagccc
    18481 cggacgagct cgtcacccac atcgtgctgc cgaagccgcc ggcggcggcg cggtcggtct
    18541 tcctgaagct cggcgccagg cgctatctcg tcatctcgat cgccatggtg gcggtgacgt
    18601 tcgttctcga tgatgaaggg cggatcgccc tgctccggct cgcggtcggg gcctgctcgc
    18661 cggcggcggt gcgcctctgc gagctggagg agcggctcct gggcctgacg cccgaggccg
    18721 cggcggctgc ggtcgggcct gcggatctgg ccatgctgac gcccatcgac gatatccgcg
    18781 cgagtggcgc ctaccgccgc catgccgcgc tcgtgctgct gcgccgagcg ctcggcggcg
    18841 agcgcctgca ggtggcggca tgaacgccct ctcaccggtc gaggtgggca tcggctttcg
    18901 gctgaatggt cggccggtgg tgatcgatgc gccgccggcg acccggttga gccgcgtcct
    18961 ccgcgaaaac ctcgggctga ccggcaccaa ggtcggctgc gatgccggcg attgtggtgc
    19021 ctgcacggtg ctgatcgacg gtcggcaggc ctgcgcctgc ctcctgccgc tcggtcaggt
    19081 cggggggcgc tcggtgacca gcatcgaggg gctcgcggcc gagccccgct tcagccggct
    19141 gcagcaggcg ttcgtcgagg gcggggccgt gcagtgtggc atctgcaccc ccggcatgct
    19201 gatggccgcg gcggatctgc tcaaccggtg cgagcggcca ggccggggcg agatcgagga
    19261 cgcgctgggt ggcgtgctct gccgctgcac cggctaccga cagatcgtgg cggctgtcgc
    19321 cgaggcgatt gcgccgcgag cgcccgcccc gcggcccgcc ggccccgtcg tgggggcgcg
    19381 gatcgcccgg ctcgacggcc gggacagggc cgcgggcaca gagcgcttcg gtgcggacgg
    19441 cgtccccgcc gacacctggc ggctgcgcgt ggtgcgatcg ccgcacatgc gggcgcgctt
    19501 cagcctcggc gatctcgccg cggtcgagcg gcagcatggc gtgcgggtgc tgacagcggc
    19561 cgatgtgccg ggcgtcaacg gcttcgccat ctttcccgac cgcaaggacc agccggtctt
    19621 cgccgtcgat catgtgcggt ttcgcggcga ggcggtgctt gctttggtcg gtccggccgc
    19681 ggcgctggcc gggatcaggg acgccgggct gccgatcacc tgggagccgc tcgagcccgt
    19741 gagcttcgcc gccgcccgga cgggggaggc gccggccctg cacggggacc ggccggacaa
    19801 cgtgctgacc gaggggcggc tcgagccggg cgatgccgcg gcggcgctgg cggcggctgc
    19861 ggtcacggcc gagatcgcca tcgagacgag tttcgtcgag cacgcctata tcgagcccga
    19921 ggccggctgg gccgtccgcc ggggtgatcg tctggagctc accgtctcga cccagagccc
    19981 gtacatggac cgggacgagg tggcgaacgt cctcggcgtg gcgccggagc aggtcaggat
    20041 ccgacccacg gcctgtggcg gcggcttcgg cggcaagctg gacgtggcgg tgcagccgct
    20101 cttggcggtc gccgcctggc ggctcgaccg gccggtggcc tgcacctaca gccgcgccga
    20161 gagcatggcc tcgaccacca agcgtcatcc ggcggcgatc gtcgcgcgcg ccggcgccga
    20221 tgcctcgggg cggatcacgg cgctcgactt cgcgggcgat ttcgacaccg gcgcctatgc
    20281 ctcatggggg ccgacggtgg cgggccgggt gccgatccac agccccgggc cctaccgcgt
    20341 cgccaacctt cgggcgcgat cgcgggcgat cctgacgaac gcgccgccga gcggggcgtt
    20401 tcgtggcttc ggcgtgccgc agacgctgct cgcggtcgag caggcctatg acgagctggc
    20461 gctgaagctc caaatcgacc cgctcgagtt ccggctccga aacgcgcttc ggccgggcga
    20521 tgccaccgcc accgggcaga tgctggaaag cgtcggcatg gtcgagtgcc tggaggccct
    20581 gcggccacgc tggcgggagc tgcgagcagc ggcaaaaggc agtgggcccg tgcggcgcgg
    20641 ggtcggcatc gccagcgtct ggtacggctg cggcaatacc gggatgagca atccgtcgag
    20701 ccagcgggtc agcctgctgc gcgacggccg gatcatgttc tggaacggcg tgcaggatat
    20761 cggccagggc tcgagcacca tcatggcgca gatcctggcg gacgcagtcg gtgtgccgct
    20821 cgacgccatc gacagctcct cggccgacac cgacctcacg ctcgattgcg gcaagacgtc
    20881 cgcctcgcgc cagactttcg tctccggacg ggcgacgcag ctcgctggcg aggcgttgcg
    20941 cgcgcaactg ctcaggctgg ccaatgccgg cggcgacgcg cggatcgagc ccggcgccgg
    21001 atcggtgcgg gtggtcgagg gcggccacga gcgccgcatc gacctggcgc tgctgccgta
    21061 cgtcgagggc tgcggcccgg aggttgcgct cgacgcgcgc gccagcttcg atcccccggc
    21121 gacgccggtc ctggacaatc ggggctcgcc ctatgcctcc tatggctttg gcgcgcagat
    21181 cgcgcaggtc gcggtcgaca tcgagctcgg cacggtcaag gtcgagcgga ttgtcgccgc
    21241 ctatgatgtc ggccgcgcca tcaacccgga gctggtcgag gggcagatca tcggcggcgt
    21301 ggcgcagggt ctgggcctgg cgctcatgga ggagttcatc cccgggcgga ccgagaatct
    21361 gcacgactac ctgatcgcta cggtcggcga catgcccgag gtggaggtgc atctggtgga
    21421 gaaagcggag ccgcttggcc cattcggtgc caagggtgtc ggcgagcacg cgctgattcc
    21481 gacagcaccg gccatcatga acgccatcca ccacgccacg ggcgtgcgga tcacgaagat
    21541 tccggccatt ccgcatcgcg tgcatgcggc gctggtggcc gcgggagtga gcccgtgacc
    21601 gacatgaccg agcggttcgg cgcgcgcgag gccgatggca tcgtgcgctg cgacgcctgc
    21661 ccggtgctct gccgcatcag gcccgggcgg tcaggcgcct gcggccgcta cgccaacgag
    21721 gacggctcgc tcgtccgcac cgatcccgtg ctcttcgccg agcgccatgt cgacgagggc
    21781 ggtgcgctgg tcccgttcgc cgagggcgac cgggacggtg ccgtcggccc gagcgtcgag
    21841 accttcgtca ccggcatcgg ctcgggcacg acctatcccg actacaagcc cgcacccttc
    21901 atcgtcagct cgacctatcg gggtgtcgac acggtcacgg tggtgtccga ggggatcttc
    21961 tcctattgcg gctgcaaggt gaagatcgac accgaccggc atctcggggc ggagcggagt
    22021 gccatcaccg tcaagggcga gcccgtcggc cacgtgacga ccgccgagta cggctcgcag
    22081 atgctttcga tcggcggcgt gcatcacctg accggcacga ccaagaaggc cggcaacgtg
    22141 acctgcgaca cgctggtcag gctctgcggc ggcgagccgg tcgagctcga gatcgaggcg
    22201 ggcagcagcg tcgtcgtcca ggccggcgcg gcaccgatcg tcaacggcaa gccggaggcg
    22261 cggatgcggg tcggctgcgg ctccgcaacc gtcggcatct tcgccaagca gtggtatggc
    22321 catgtcgacg aggtgatcgt cctcgacgat cacatcaccg gcgtgctcac cgagcaccag
    22381 gccggccgct gtctcgacat gccgccttcg ggcgtgcgga tcgagggccg caaatccacg
    22441 ccggggcgct atttccaggt ggccaatccg ggcctgggct ggggtgggac ggacatcacc
    22501 gacccgttca gcgtgatccg cgacatcgac cgcaagctcg cctggcccgg catgcgcctg
    22561 ctgatcacct cgaccacggg cgaggatgcg gtctggtgcg tgctcgacga ggatttccgg
    22621 ctgcgggagg cggagatgcc ggcggcggtg cgccggacgg tcgagcggat cggcgagaac
    22681 tgcgagcctt ccctctgctc cgtcctcttc atggccggcg ccggcggctc gctgcgcgcc
    22741 ggggtctcgg agaatccggt gctgatcacc cgctcggtca aggccggcga gacccgggtc
    22801 acgatgggcg gggcacccgt ctatgtctgg ccgggtggcg gcatcaccgt catggtcgat
    22861 gtcgagcggt tgccgaagcg cgccttcggc tacgtgccga caccggccct ggtcgcaccc
    22921 atcgagttca ccctgccgct cgagctctac acggcgctcg gcgggcacgc nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cacccatcga gttcaccctg ccgctcgagc
    23101 tctacacggc gctcggcggg cacgcggacc atgtccagtc gctggacgag gtgcttcgat
    23161 cgcatgggca gtcggcgcga cgcgaggcct gggcgccgcg caatccctgg ccgctcgggc
    23221 ggctgacgcc atgacggccc gcgcgatggc gtggggtggt cccgccgcgg cccggctcgc
    23281 cggcaaccgt ctgcacctgg cgcacgggcc gatcgatctg gtcatccggg ccgatgggcc
    23341 gtcggcttcg gtcgagcgcg cctatcgggc ggccggcgcc gcgttcgccg gcctgctcga
    23401 gagcctggtc gccgtcctgc cccggctccg cgctcccgtc ggctcggccg ccggcctgcc
    23461 gccggcggca cgcctgatgc aggcggcgac agcacccctg gccaccgacc tcttcgtcac
    23521 gccgatggcg gccgtggcgg gggcggtggc cgacacggtg atgacggcga tccgcgccgt
    23581 gcccgggatc gagcgcgcct atgtgaacaa ccggggcgac atcgcggtcc atctgacccc
    23641 cagcgccagt atcgagatcg gcgtggtgcc ggagctgcgg cggccgctca tggcggcgag
    23701 cctgcgcctt cgccacgacg acggcatcgg cggcgtggcg acgagcggct gggacgggcg
    23761 gagcttctcg ctcggcatcg ccgatgcggt gacggtgctg gcggcgagtg cggcccaggc
    23821 ggacgcggcc gcgaccctca tcgccaacgc ggtcgatgtg gacgatttaa cgatcacccg
    23881 cacgccggcc accgagctgg acccggacag cgatctgggc cggctgctcg tgaccacggg
    23941 cgtcggagtc ctggacgacg ccgcgatcgc cgcggccctc gacaacgggg cacgctacgc
    24001 gcaaacgctg ctggagcgcc ggctcatctc tgcggccatg ctggtcctcg agggacgcgc
    24061 ccgcatcgtc ggcaggatcc ggggggagat cgcatgttcg agctgagaaa gacgcaggtc
    24121 acggtcgagg agatccacca cgacggcggg ccgcacctcg atcggccctt gctgcgcggt
    24181 gccgtggcgg cggtgttcaa gaacccgttc gctggccggt tcgccgacgc tggcgaattc
    24241 gtcgaggcga tggacgccct gaagccgctc ggcctggaga tgtcgaggaa gctgatccag
    24301 gcgctgggcg gtgatcccag gcggatcgag agctacggca aggggtcgat cgtcgggtca
    24361 gacggcgagc tcgagcaggg tgcgctttgg catgtgccgg gcggctacgc catgcgcgag
    24421 ctgctcggcg acgcccgggc catcgtgccc tcgagcacga agctcggcgg catgggcgct
    24481 gccctggacg tgccgctgca tcacatcaac gccgcctatg tccgcagcca tttcagcgcc
    24541 atcgaggtcc gggttgcgga tgcgccgcgg cccggcgaga tcgtcttctg cctcgccatg
    24601 accacgggcg gccggccgca tgcccgaatg ggcggcctcg aggcggcgga catcaagggg
    24661 caggacgggc tgcgctgagc ggccgagggg ggaagggagg ggctatcatg gccttcgtgc
    24721 gcaacaactg gtacccggcg ggcttcgcca gccgcatggg cgaggggctg cacaagcgcc
    24781 ggatgctcgg cgaggacctg gtgatctggc agcaggccga cggctctttc gcggcgctcc
    24841 aggatcgctg ccctcaccgc ttcctgccgc tctcgatggg ctgcctcgag cgcgacacga
    24901 tccgctgcgg cta
//
LOCUS       DAOTFI010000032        24817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2344_length_24817_cov_7.814635, whole genome shotgun sequence.
ACCESSION   DAOTFI010000032 DAOTFI010000000
VERSION     DAOTFI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 24817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24817
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2344_length_24817_cov_7.814635"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="P5558_04830"
     CDS             complement(<1..227)
                     /locus_tag="P5558_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRY23665.1"
                     /translation="MLTRRFLMLAALVAPAFAAARPLAAQSAVLADVERYLNGIDTLA
                     ARFEQIAPDGSLSRGRVYIQRPGRLRFDYDPP"
     gene            complement(264..2861)
                     /locus_tag="P5558_04835"
     CDS             complement(264..2861)
                     /locus_tag="P5558_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRY23666.1"
                     /translation="MATSYFESDERTPLGDWLRGFGRRTTGLALLALAGFLVLALLGF
                     HPGDPSLNQATGAAIANPAGAVGAILADLLFQTFGRAIWLVPLVALCWGVRMVANRRA
                     AQPWLPLVALPLSLLATAAFLAGLPEPVAAEWPFRAGLGGVVGDFELLYLARHLPGQL
                     YGWLTGGAALLFGIAALGLRWREGAWAFGRVGRGSLWLGRRIGGAAQAGTAYSSRALE
                     ESRRNRRLRRAAADGTDEAAFPGVPEPVMPDTAPIEPRPGGLAHMFARIGDRLFGGAA
                     GGPLPDEPADAPAPPRRPARSRLRPEVVEEMEPGPAPVATGRSRQAPTLADEARTSSG
                     GARRPAGEAPAQASLPLNAFVLPALDMLGAARAGARPGQTRAQLDEVSRQLESVLDDF
                     GVRGEIIDAKPGPVVTLFELEPAPGTRAARVISLADDIARSLCALSVRVAVVPGRNVI
                     GIELPNEARETVYLRSLLQSDTFARSEARLALALGKDISGNPIIVDLARMPHLLIAGT
                     TGSGKSVAINAMILSLLYRLTPDQCRIIMIDPKVIELSVYEDIPHLLAPVVTEPHKAV
                     VALKWAVRQMDERYRLMSHLGVRDIFAFNRRIEQAKSRGEALTRRVRTGFEAGTGQPI
                     IEDQPIDMTPLPLIVVIVDEVADLMLTAGKEIEHAIQRLSQKARAAGVHLIVATQRPS
                     VDVLTGVIKANLPSRISFRVSSKIDSRTIMGEPGAEQLLGQGDMLYLMPGDRIMRIHG
                     PLVTDNEVERVVAHLKSQGEPDYHDEVTAENAGLDAAPEEGAPLAAEGTGDNVYEQAV
                     QIVARDGKASTSYLQRKLSIGYNSAAKLIERMEQEGLITTADHVGRRQVLMGRGGLDG
                     TQPTRDDDD"
     gene            complement(2896..4110)
                     /locus_tag="P5558_04840"
     CDS             complement(2896..4110)
                     /locus_tag="P5558_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY23667.1"
                     /translation="MWNQRFGALSEFPFRRLAALLARSGPPADLPLADLSLGEPQHAP
                     PPLLAATLAANAGLWNRYPPPAGTPAFRAAATRWLERRFELPRGWLEPDRHILPVAGT
                     KEALFLVATAATDPAGQGQRPAVLVPNPLYSVYLGAAVLAGAEPVLMPASASTGFLPD
                     LDAVAEADLARATVCYICSPANPQGVVADRTYLARALDLARRHDFLLVVDECYAEIYD
                     QEPPPSALEVAAAGGGALDHLVVMHSLSKRSSAAGLRSGFVAGDPDFLALFLRLRAYG
                     AAVQPMPVLAAAAALWDDDAHVETNRLAYRKKFDLAETALAGRAGFYRPPGGFFLWLD
                     VGDGEAAAVELWRRAHVKVLPGAYLTATGDADEPARDAATDPGTRYVRVALVHEPEVL
                     GPALDRLRAVIA"
     gene            4366..4875
                     /locus_tag="P5558_04845"
     CDS             4366..4875
                     /locus_tag="P5558_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23668.1"
                     /translation="MRAILILFFVLLLAGNARGELPDSLAGDWRGSLLVTGADAGTGL
                     PVNARLDPTRDGFEIAINAPGAKRLDARMVPGPAPDVFEVEEGGGLFGFLGDGDDRSV
                     LDGRPLLWARRAAESLVVYRLQIVADGGFELLHIVLTPAADRLEVSVQRRLDGVPEPG
                     LHGFLDRRS"
     gene            4880..5443
                     /locus_tag="P5558_04850"
     CDS             4880..5443
                     /locus_tag="P5558_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23669.1"
                     /translation="MTHFCRRLALAALLCLTTLPGPVSAQSLTDYFGAFVGRAEVRDT
                     GEGLVETRDIDIEIGPFRRTGFRLRWTNVTLVDGRRDVPGVKRRVNEVIFEPHDGSRL
                     FVEASVANLFQRQEEMSPLAGDPVRWAKLDENGMHVFSFAILEDGQYELQTYTRQLVD
                     DGLELTFERVVDGEVLRRIDGWAVRAD"
     gene            5433..6449
                     /locus_tag="P5558_04855"
     CDS             5433..6449
                     /locus_tag="P5558_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY23670.1"
                     /translation="MRTDRRRALVGAAALLGLGAVPAVGQDMRLFRIGTGGIAGTYYP
                     IGGLIATIISQPSGARSCDAGGSCGVPGLAAIVQSSNGSVDNIDGIAEGRIESGFVQS
                     DVAYFAQTGTGAFAGRQPVESLRLLASLYVESVHLVVAAESGIESVGDLRGRRVSLDS
                     MGSGTQIDALLLLEGHGLTTDDVVAYHAKPEQAVRLMAQGALDAFFIVGGYPIPSVVE
                     AASDLGARLAPIDGEAAAALVETYPFFSADLIPGGVYAGVGPTPTLAVVAQWLVDARL
                     DEALVHDICRALWHPTARALLDGGHAKGRQITLETALDGAAIPLHPGAERYYREVGLL
                     PPGG"
     gene            complement(6459..7223)
                     /gene="pgeF"
                     /locus_tag="P5558_04860"
     CDS             complement(6459..7223)
                     /gene="pgeF"
                     /locus_tag="P5558_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRY23671.1"
                     /translation="MKLEALALARLDGVAHGFLGRRGGVSEGPFASLNVSLRNADDTA
                     NALENRRRAVAALGQADHPLLIARQVHGTRCAIVEAAFDPLAPPEADALATATPGLVL
                     GVTTADCAPVLFADPAAGVVAAAHAGWRGAKDGILEATVEAMRRLGAVPGRTVAAIGP
                     CIAVASYEVGAEFEAAFLAAEPESRRFFRAVAAGQRHFDLAGYCVDRLARAGIATVER
                     LDRDTCAEAEDFFSYRRATRDGERHFGLQVAAISRF"
     gene            complement(7220..8371)
                     /locus_tag="P5558_04865"
     CDS             complement(7220..8371)
                     /locus_tag="P5558_04865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRY23672.1"
                     /translation="MTVAAASPAGLDDDVASPLGRELVALIEAHGPIDVATFMALALG
                     HPTHGYYSSRAALGAGGDFVTAPEASQLFGELIGAALATAWQQAGGAPARLVELGPGN
                     GTLLADLWRATAGVKGFHAALDVHLVETSPLLRRRQAERLGAVPGLAWHAALDEVPAD
                     RPLLVVANEFLDALPIRQLIRLEDGWHEIQLGLDARGRLAFAPDPRPSPLALRLPAAP
                     IGSVLELSPAREAAVAGIAHRLADQGGLALVVDYGERDPAPGSTLQAVSRHRRVDPLT
                     RPGEVDLTSRVHFAPLINVAGAAGPTVFGPVPQGLFLQRLGIDLRLGHLLAGASAAAA
                     TGLRLGHARLTEPAAMGELFQVLTIDGWGNGPPAGFVAAEANAPVRARP"
     gene            complement(8368..9183)
                     /gene="lgt"
                     /locus_tag="P5558_04870"
     CDS             complement(8368..9183)
                     /gene="lgt"
                     /locus_tag="P5558_04870"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRY23673.1"
                     /translation="MSFLAIPFPAFDPVLIQLGPFAIRWYALAYIAGLLGGWQLLKRM
                     VARPGWTMTPEQVDDLLFFATLGVILGGRLGFVLFYHPLYYLSNPLQILAVWQGGMSF
                     HGGLVGVLVACWLFARSRGIPALEVGDAVATVTPVGLFFGRLANFINAELWGRPTELP
                     WGMVFPNAGPLPRHPSQLYEAALEGILLFIVLQVMARGARDPATAGRLGGTFLLGYGL
                     CRAFVELFREPDAHLGLLFGFITMGQLLSLPMILLGLVLIAWSWRDRGRRVAS"
     gene            9366..9710
                     /locus_tag="P5558_04875"
     CDS             9366..9710
                     /locus_tag="P5558_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HRY23674.1"
                     /translation="MQTENRLFDDLARVASGAINTLGGVREEIELRVKERLERLAGEM
                     DLVTREEFDAVQAMAAKARAEQEALKARLDRLEAKLAALETADPAPEARARPKRRGAE
                     GPDPATEAGSAG"
     gene            9874..10377
                     /locus_tag="P5558_04880"
     CDS             9874..10377
                     /locus_tag="P5558_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRY23675.1"
                     /translation="MTSLLSEFAVPPRNPLELVEKMAVANDWPFHRQSDEELAAEISG
                     QWCHYKLWFAWHPEMGVLHLSCALDMKVPAKKRPDFYHVLAIANEKLWVGHFEIWPEE
                     DLPVFRHASLFREGSTASASLIEDLVEIALNECERFYPAFQFVLWGGKEPQEAILAAM
                     LETEGEA"
     gene            10377..11210
                     /gene="proC"
                     /locus_tag="P5558_04885"
     CDS             10377..11210
                     /gene="proC"
                     /locus_tag="P5558_04885"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRY23676.1"
                     /translation="MATIPGPLLLVGGGRMGEALLGGWLRQGLASGSATVIEPDPGRA
                     AALTAQGLAVVPSAADLPPGFRARILVLAVKPQVMADVLPALPGHLAPGGLVLSIAAG
                     KPLAVFEAAFGAVTPIVRVMPNTPAAIGRGISALFANPATDAANRALAERLMQAVGET
                     LWLDAEAEMHAVTALSGGGPAYVCLLVEALAAAGERLGLAPELAMRLARATVAGSGAL
                     LESSDATAAALREAVTSPGGTTRAALDVLMPADGGTEAGLSGLVGEALVRAAERSRQL
                     A"
     gene            11245..11859
                     /locus_tag="P5558_04890"
     CDS             11245..11859
                     /locus_tag="P5558_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23677.1"
                     /translation="MSRKAKAQPRPDLLEAAFALIAEKGWHGFSALALAERTGASLDD
                     IHDLLPTPAALVGRLGERLDRAMLAAPPDELLELSPRERLFELLMRRLDALAPFKPGL
                     ARLRVEARAEVELALATLASLDRMAAWLLDLAGLPGRGLRGRLARRALMLAYGRVLAV
                     WLADDTPDQDDTLAELDKRLDQLERLARLGDRLTAGLGPRPRAA"
     gene            12001..13152
                     /locus_tag="P5558_04895"
     CDS             12001..13152
                     /locus_tag="P5558_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRY23678.1"
                     /translation="MALTATTNTTANTALRYLSQNNNMAASSLAKLSSGSRIVRASDD
                     AASLAIGTKIKADVTALKQAQVNAGQASSLLQVADGGLSQVVDILMRMKALAVQAQSG
                     SVSDNERGFLDKEFQALSGQIDAIAAQTKFNGTELLSGNIATAFSAIGTAIDGSGAAE
                     LKLTGGSEAGAYTVAYDDATGVLTVTQGTGVGAESQSVTLDNTDTAFTGSVAFDQFGL
                     EVAFTAFDLDTVAIAANNTATVTSGAMSFQVGVSSSDTIDVTIADIRTTALGDGTNTL
                     SDDGSGSPASIGTAAKAAGAVDIIDGALSQISEGRADVGALISRFEFAAANVATTIEN
                     LDSARSTLLDVDMAAEMSNFSSKQVLVQASVAMLAQANQMPQQLLKLLG"
     gene            13454..14605
                     /locus_tag="P5558_04900"
     CDS             13454..14605
                     /locus_tag="P5558_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRY23679.1"
                     /translation="MALTATTNTTANTALRYLSQNNAAASSSLAKLSSGSRIVKASDD
                     AASLAIGTKIKADVTALKQAQVNAGQASSLLQVADGGLSQVVDILMRMKALAVQAQSG
                     SVSDNERGFLDKEFQALSGQIDAIAAQTKFNGTELLSGNIATAFSAIGTAIDGSGAAE
                     LKLTGGSEAGAYTVAYDDATGVLTVTQGTGVGAESQSVTLDNTDTAFTGSVAFDQFGL
                     EVAFTAFDLDTVAIAANNTATVTSGAMSFQVGVSSSDTIDVTIADIRTTALGDGTNTL
                     SDDGSGSPASIGTAAKAAGAVDIIDGALSQISEGRADVGALISRFEFAAANVATTIEN
                     LDSARSTLLDVDMAAEMSNFSSKQVLVQASVAMLAQANQMPQQLLRLLN"
     gene            14764..15009
                     /locus_tag="P5558_04905"
     CDS             14764..15009
                     /locus_tag="P5558_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23680.1"
                     /translation="MPAEAAATSLERVVQQAARDLFPGREVAVESFFDKASGRFVHRI
                     ADGESGEILAQTPSDSLLRFFASGRAPLGGPLLRLEA"
     gene            15027..16724
                     /gene="fliD"
                     /locus_tag="P5558_04910"
     CDS             15027..16724
                     /gene="fliD"
                     /locus_tag="P5558_04910"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRY23681.1"
                     /translation="MASSISFGSLSTSSGSPRLSGTSSKLDTEAVLQATYEAKRLPAV
                     RLGNKTTTNETKIAAYESMQGLLESLRSAANGLRNPPGFLGARDNAFETKQAFLGSST
                     TTAPGEIVGVALANTAQAGSFDLRVTQLATAEKLATPGAAAANATLADAWNAGDPFSG
                     TIELGVAGGPTAVIDLDGDLGLYDLRDRINAVRSTTGVGASVLKVADNDFRLVLAAEE
                     TGKAITLNDTSGLTGGFSTTTLQTAQPAIFEIDGVSVTRTSNQVDDLMGGVTINLFKA
                     EPGTTVAVSVEPSLGDIKSRIVAFVEAFNALRGFVNEQSAVDASGGPAADAVLFGDRT
                     MRGIMTGLGGIVGGSVAGLDADALGSFRAIGIELDATNQLEIDDAKLDNALLTKLDDV
                     RDIFEFRYRSSSSELAIFARSNALAATTMQLEITDADNDGTPEAVTINGQAAEIAGST
                     IKGAAGTAFEGLEFIWTGRGSTVIDVEASQGLADRVFNFVKPAVDPLDGSIPRAIDSL
                     RDLNGNYAKQMERIEERATRARDLLVERFSAMEAALSLANAMLAQIRAQIDAMTQDS"
     gene            16824..17258
                     /gene="fliS"
                     /locus_tag="P5558_04915"
     CDS             16824..17258
                     /gene="fliS"
                     /locus_tag="P5558_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRY23682.1"
                     /translation="MQQRARAMTAYQTAALTVTPARAIVMLYDGAVQRLQQAGRAITE
                     GRIEERYRLVGKAHAIVHGLQCQLDFAAGGEVALLLDRYYAYLLHRMTQINVRNDPAI
                     CDEVIARLRDMRASWAAIAETGNAAPAPPPVQADHRPIVQLG"
     gene            complement(17525..17785)
                     /locus_tag="P5558_04920"
                     /pseudo
     CDS             complement(17525..17785)
                     /locus_tag="P5558_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18582..18956)
                     /locus_tag="P5558_04925"
     CDS             complement(18582..18956)
                     /locus_tag="P5558_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY23683.1"
                     /translation="MPRTVEDMLAEANTAVPPITPAEARALLADPGTLVGDVRDAPEV
                     AASGKVRGAVNISRGMIEFRADPASPCRDERLSPEKTVIVYGASGGRSTLVGKTLQEL
                     GYQDVSDLGAFKGWVESGGEVE"
     gene            complement(19018..20115)
                     /locus_tag="P5558_04930"
     CDS             complement(19018..20115)
                     /locus_tag="P5558_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRY23684.1"
                     /translation="MNEFQVAVLPGDGIGREVMAVALEVLEVLESRFGLGLALQHHDG
                     GAAYYRETGRDLAPGVMEACRSADAILFGAMGLPDVRFPDGTEIAPHLDMRREFGLFA
                     GVRPVRALPNTPAILAHPRAAEIDFVIIRESMEGLFASRGVGYVEDDRIARETMVITR
                     DNCERLFDFSFRLAERRKAKGRPGRLTCVDKANVFVAMAFFRKIFDERAKGFPDIRAD
                     HHYVDATALDLLRKPWEFDVMVMENMFGDIISDLGGGLVGGMGLAPCAEIGLEHALFQ
                     PSHGSAPDIAGQGIANPTAMLLSTAMMLDWLGERHGQESMVTAGATLERAVDLAFAGG
                     AVPRDLGGRHTTTELAKAVIDQLDAAASAIG"
     gene            20308..23145
                     /locus_tag="P5558_04935"
     CDS             20308..23145
                     /locus_tag="P5558_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY23685.1"
                     /translation="MTATIDTSPKIADEIVCTTCYMCACRCGIRVHKLDGEIRYIDGN
                     PEHPVNRGVLCAKGSAGIMHHNSPARLTKPLKRVGERGEGRFEAIEWDEALAIAEGWL
                     RGVRETDPRQLAFFTGRDQSQALTGFWASQFGTCNYAAHGGFCSVNMAAAGLYTIGGS
                     FWEFGEPDFEHARYFLIFGVAEDHDSNPLKLGMAELKKRGAKIVSVNPIRTGYSAIAD
                     EWIGIRPGTDGLFVMSLMHELINAGRIDVEYLRRYSNATALVIDAPGDADDGLLARDG
                     DGRLLVGLQGKATVAPTGPAVQAALQGRYTLADGRAAVPVFELLARRYAAAEYSPDAV
                     AERCGIPAATIRRIAAELAAAAFDAPVVLERPWTDTNGMRHERMIGRPVAMHAMRGIS
                     AHSNGFHTCRAIHLLQVLLGTIDTPGGFRFKTPFPRPTPPGPQPAGRADQMAPGKPMA
                     GPPLGFPRGPADLILDEAGGPRRIDKAFSWDAPISAHGMMHSVIGNAFRGDPYRIDVL
                     FMFMANMAWNSAMNTTETMAMLEAKNADGSHVIPKIIYADAFYSETVAYADLVLPDTT
                     YLERHDCISLLDRPIGSADAVADAIRQPVEVPDRDVRPFQDVLIDLGARLGLPAFVDA
                     SGAPRFPGGYADYMVNHERKPGVGSLAGWRGPNGDQAGKGAVNPDQLERYKANGCFFS
                     LPIPEEATYFKHANAAYLKLAVELGLIDEAAPITMQLYCEPLQRFRLAARGHGALQPP
                     ASKRERIETYFDPLPIWYMPFDEAAVDAQAFPLHAVTQRPMFMYHSWGGQNPWLRQIL
                     DRNALYLNRRTAAGLGIEEGDRVAVTSRHGRVEGRAKLMDGVHPSTVWTWNAIGKRRG
                     AWGLQGDIPEASEGFLLNSLIDDLLPEQDGGYRYANADPITGQAAWYDLRVRVEKLGA
                     EARAPEPAALARPPGAMPVPETLSYRVRGGA"
     gene            23142..23882
                     /locus_tag="P5558_04940"
     CDS             23142..23882
                     /locus_tag="P5558_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY23686.1"
                     /translation="MTSLPANPSAKRLGLVIDLDTCVGCQACVVHCKEWNTSSGASAL
                     SDTDPYGEAPSGAWLNRVHGFELDDSASGGCGSTVHFPRSCLHCENAACVTVCPTGAS
                     YKRAEDGIVLVDESKCIGCGLCAWACPYGAREMDRVARVMKKCTLCVDRIYNENLPVE
                     EREPACVRTCPARARHFGDLGDPESDVSLLVAERGGVALMPELDYRPTNRYLPPRRPV
                     PAADRPAALEPAPGGFLGWLDRLLADQP"
     gene            23893..>24817
                     /locus_tag="P5558_04945"
     CDS             23893..>24817
                     /locus_tag="P5558_04945"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HRY23687.1"
                     /translation="MHPAPSIIFFTAASGAGYGLLVFAALAGLIGLVPVGSASGFALL
                     LPALLLVAAGLLSSAGHLGHPERAWRAFSQWRSSWLSREGVAAVVSFVPALGLAWAWA
                     LNGRLAPLLALATILMAALTVYCTAMIYASLKPIPRWHHRLVPPVYLGLGAASGSAVG
                     IAAAAWTGASVLPLAPLVALALLVGWGLKWRYWQSVDTQPATATTADAVALGDRGTVR
                     LVEPPHTERNYILDEMGFRIARKHGAKLRRHALIAGLALPLVALALALLLPQVPAALL
                     LSIGAGLALVGVAIERWLFFAEAEHLVMLYYG"
BASE COUNT         3711 a         8833 c         8747 g         3526 t
ORIGIN      
        1 ggcgggtcgt agtcgaagcg caggcgcccc ggccgctgga tgtagacccg gccgcggctg
       61 aggctgccgt ccggggcgat ctgctcgaag cgggcggcca gcgtgtcgat gccgttcagg
      121 tagcgctcga catcggcgag gacagcgctc tgcgccgcca ggggccgggc ggcggcaaac
      181 gccggggcga ccagcgcggc aagcatcagg aaacggcggg tcagcaagcg gcctcgctcc
      241 agtgaatggg acgacagggg tgctcagtcg tcgtcgtcgc gggtcggctg ggtgccgtcg
      301 agcccacccc ggcccatcag cacctggcgc cgcccgacat ggtcggccgt ggtgatcagg
      361 ccttcctgct ccatgcgctc gatcagcttg gcggcgctgt tgtagccgat gctgagcttg
      421 cgctgcaggt agctggtcga cgccttgccg tcgcgggcca cgatctgcac ggcctgctcg
      481 tacacattgt cgcccgtgcc ttcggccgca aggggggcac cctcctccgg ggccgcgtcg
      541 aggccggcat tctcggcggt cacctcgtcg tgatagtcgg gctcgccctg cgacttgagg
      601 tgggccacga cccgctcgac ctcgttgtcg gtgacgagcg gcccgtggat gcgcatgatc
      661 cggtcgcccg gcatgaggta gagcatgtcg ccctggccga ggagctgctc ggcacccggc
      721 tcgcccatga tcgtccggct gtcgatcttc gagctgacgc ggaagctgat gcgcgacggc
      781 aggttggcct tgatgacgcc ggtcagcacg tcgaccgagg gccgctgggt cgcgacgatc
      841 aggtggacgc cggcggcgcg cgccttctgc gacaggcgct ggatggcgtg ctcgatctcc
      901 ttgccggcgg tgagcatgag gtcggccacc tcgtcgacga tgacgacgat caaaggcagc
      961 ggcgtcatgt cgatgggctg gtcctcgatg atcggctggc cggtgccggc ctcgaagccg
     1021 gtgcgcacgc gccgggtgag cgcctcgccg cgcgacttcg cctgctcgat gcggcggttg
     1081 aaggcgaaga tgtcgcgcac gccgagatgc gacatcagcc ggtagcgctc gtccatctgg
     1141 cggaccgccc acttgagcgc caccaccgcc ttgtgcggct cggtgaccac cggggccagg
     1201 agatgcggga tgtcctcgta gaccgacagc tcgatgacct tggggtcgat catgatgatc
     1261 cggcactggt cgggggtcag ccggtagagc agcgagagga tcatcgcgtt gatggcgacc
     1321 gacttgccgg agcccgtggt gccggcgatc aggagatgcg gcatgcgggc gaggtcgacg
     1381 atgatcgggt tgccggagat gtccttgccg agggcgaggg cgaggcgcgc ctcgctccgg
     1441 gcgaaggtgt cggattgcag cagcgagcgg aggtagacgg tctcgcgcgc ctcgttcggc
     1501 agctcgatgc cgatgacgtt gcggccgggc acgacggcga cacgaacgga gagcgcgcag
     1561 agcgagcggg cgatgtcgtc ggcgaggctg atcacccggg cggcgcgcgt gccgggggcg
     1621 ggctcgagct cgaagagcgt caccaccggg ccgggcttgg cgtcgatgat ctcgccgcgg
     1681 acaccaaaat cgtcgagcac gctctcgagc tgccgcgaca cctcgtcgag ctgcgccctg
     1741 gtctggcccg gccgcgcccc ggcccgggcg gcacccagca tgtcgagcgc cggcaggacg
     1801 aaggcgttga gcggcaggct cgcctgtgcc ggcgcctcgc cggccgggcg gcgggcgccg
     1861 ccggaggagg tccgagcctc gtcggcaagg gtgggcgcct gccgcgagcg gcccgtggcg
     1921 acgggcgccg ggcccggctc catctcctcg acaacctcgg gccgcagccg cgagcgggcc
     1981 gggcggcgcg gcggcgccgg cgcatcggcc ggctcgtcgg gcagcggccc gcccgcggca
     2041 ccgccgaaca gacggtcgcc gatccgggcg aacatgtggg cgagaccgcc cggccgcggc
     2101 tcgatcggcg ccgtatccgg catgaccggc tccgggacgc ccggaaaggc ggcttcgtcg
     2161 gtgccgtccg ccgcggcgcg gcgcaggcgc cggttgcgtc ggctctcctc gagcgcccgg
     2221 ctggaatagg ccgtgccggc ctgggcggcg ccaccgatcc ggcgaccgag ccagagggag
     2281 ccccggccga cccggccgaa cgcccaggcg ccctcgcgcc agcgcaggcc gagcgcggcg
     2341 atgccgaaga gcagggcagc gccaccggtc agccagccgt agagctggcc cggcagatgc
     2401 cgggcgaggt agaggagctc gaaatcgccg acgacgccgc cgaggccggc acgaaacggc
     2461 cattcggccg ccaccggctc gggcaggccg gccaggaagg cggcggtggc gagcagcgag
     2521 agcggcagcg ccacgagcgg cagccagggc tgggccgcgc gccggttggc gaccatccgc
     2581 acgccccagc agagggcgac cagcggcacc agccagatgg cccgcccgaa ggtctggaag
     2641 aggagatcgg cgaggatggc gccgacggca cccgccggat tggcgatcgc tgcacccgtc
     2701 gcctggttga gcgagggatc cccgggatgg aagccgagca gcgccaggac gaggaacccc
     2761 gcaagggcca ggagcgcgag cccggtggtc cgccggccga agcctctcag ccagtcgccc
     2821 aatggggtcc gctcgtcgct ctcgaaatag gatgtggcca tgcgcgcggt tctctctgtc
     2881 gctgcggtcg cagtgtcaag caatgacggc ccgaagccgg tcgagcgccg ggcccagcac
     2941 ctcgggctcg tggacgaggg cgacgcgcac gtagcgcgtg cccggatcgg tggcggcatc
     3001 ccgggcgggc tcgtcggcgt cgcccgtggc ggtcaggtag gcgccgggca gcaccttgac
     3061 atgtgcccgg cgccagagct cgaccgccgc cgcctcgccg tcgccgacat cgagccagag
     3121 gaagaagccg ccgggcgggc ggtagaagcc ggcgcggccg gcgagcgcgg tttccgccag
     3181 atcgaatttc tttcgatagg ccaggcggtt ggtctccaca tgcgcgtcat cgtcccagag
     3241 ggcggccgcc gcggccagga ccggcatggg ctggacggcg gcgccatagg cgcgcaggcg
     3301 gaggaacagg gcaaggaaat ccgggtcgcc ggcgacgaag ccgctgcgca gcccggcagc
     3361 gctcgaccgc ttggagagcg agtgcatgac gacgaggtgg tcgagcgcgc ccccgccggc
     3421 ggcggccacc tcgagggcgc tcggtggcgg ctcctggtcg tagatctcgg cgtagcactc
     3481 gtcgacgacc agaaggaagt cgtggcgacg ggccagatcg agcgcccgcg ccagataggt
     3541 gcgatcggcc accaccccct gcgggttggc cggcgagcag atgtagcaga cggtggcccg
     3601 cgccagatcc gcctcggcga ccgcatcgag atcgggcagg aaaccggtcg aggcactcgc
     3661 tggcatcagc accggctccg ccccggcgag cacggccgca ccgagataga ccgagtagag
     3721 cgggttgggg acgagcaccg ccgggcgctg gccctgccct gccgggtcgg tcgcggcggt
     3781 ggcgacgagg aagagcgcct ccttggtgcc ggccaccggc aggatgtggc ggtcgggctc
     3841 gagccagccc cggggcagct cgaaacgacg ctcgagccag cgggtcgccg cggcgcggaa
     3901 cgccggcgtg ccggccggcg gcgggtagcg gttccagagc cccgcattgg ccgcgagcgt
     3961 ggcggcgagc agcggcggcg gcgcatgctg cggctcgccg agcgagagat ccgcgagcgg
     4021 cagatcggcc ggcgggccac tccgggcgag cagcgctgcc aggcggcgaa aaggaaactc
     4081 gctgagcgcc ccgaagcgct gattccacat gattccggtt cttgcctgcc cggctggccc
     4141 ggcggttcgc cgctgccagc accgttctcg gatacgcccg tgtcggcccg tatccggcat
     4201 gagctggaca gggcttgtac ccatacctta acactgaggg gcaacagctt caacttgcgt
     4261 atgattcggt gagcaggcgc tgttcgacgg cggaccggat gacgatcggc gtcgaaaagg
     4321 ctacgatgtc ccgatcagcc gatggactgc cgccacgagg gccgaatgcg cgccatcttg
     4381 atcctcttct tcgtgctgct gctggccggc aacgcacgcg gcgagctgcc cgattcgctc
     4441 gccggcgact ggcgcggctc gctgctcgtc accggcgccg acgcgggcac cgggctgcct
     4501 gtcaacgcac ggctcgaccc gacccgcgac ggcttcgaga tcgcgatcaa cgcgccgggc
     4561 gccaagcggc tcgacgcgag gatggtgccg ggcccggcgc cggacgtgtt cgaggtggag
     4621 gaaggcggcg gcctcttcgg ctttctcggg gacggcgacg accggtcggt gctggacggt
     4681 cgcccgctgc tctgggcgcg gcgagcagcc gagagcctcg tcgtctatcg gctgcagatc
     4741 gtcgcggacg gcggcttcga gctgctgcac atcgttctca cgccggcggc ggaccggctc
     4801 gaggtcagcg tgcagcggcg cctggacggc gtgcccgagc ccggtctgca cggttttctg
     4861 gacaggcgga gctgagccga tgacgcattt ttgccgtcgt ctcgcgctcg cggccctgct
     4921 ctgcctgacg accctgcccg gacctgtgtc ggcgcagtcg ctgaccgact atttcggcgc
     4981 cttcgtcggt cgcgccgaag tccgcgacac gggcgagggt ctggtcgaga cgcgcgacat
     5041 cgatatcgag atcgggccgt tccggcgcac cggctttcgc ctgcgctgga ccaatgtcac
     5101 gctggtcgac ggccgccgcg acgtgccggg cgtgaagcgc cgggtcaacg aggtgatctt
     5161 cgagccgcat gacggcagcc gcctcttcgt cgaggcatcg gtcgccaacc ttttccagcg
     5221 gcaggaggag atgtcacccc tggcgggcga tccggtccgc tgggcgaagc tcgacgagaa
     5281 cggcatgcat gtcttctcct tcgccattct ggaggacggc cagtacgagc tgcagaccta
     5341 cacccgccaa ctggtcgatg acgggctcga gctgaccttc gagcgggtgg tcgacggcga
     5401 ggtgctgcgg cgcatcgacg ggtgggcggt ccgtgcggac tgaccggcgc cgggcgctcg
     5461 tcggggcggc ggccctgctc ggcctcggcg cggtgccggc cgttggccag gacatgcgtc
     5521 tcttccgcat cggcacgggc gggatcgccg gcacctacta tccgatcggc ggcctgatcg
     5581 cgacgatcat cagccagccg tcgggggcgc gcagctgtga cgccggcggc tcctgcggcg
     5641 tgccggggct cgccgccatc gtccagtcct cgaacggctc ggtcgacaat atcgatggca
     5701 ttgccgaagg ccgcatcgag agcggcttcg tgcagagcga cgtggcttat ttcgcccaga
     5761 ccgggaccgg cgcctttgcc ggccggcagc cggtcgagag cctgcggctc ctggccagcc
     5821 tctatgtcga gagcgtgcat ctggtggtcg cggccgagag cggcatcgag agcgtcggcg
     5881 atctgcgcgg ccgacgggtc agcctcgaca gcatgggctc gggcacgcag atcgatgcgc
     5941 tgctgctgct cgagggccat ggcctgacga ccgacgacgt cgtcgcctac cacgccaagc
     6001 cggagcaggc ggtgcggctg atggcccagg gtgcgctcga tgccttcttc atcgtcggcg
     6061 gctacccgat cccctcggtg gtcgaggcgg ccagcgatct cggcgcgcgt ctcgccccga
     6121 tcgacggcga ggcggcggcg gccctggtcg agacctatcc cttcttctcg gctgacctga
     6181 ttccgggggg cgtctatgcc ggggtcgggc cgacgccgac gctcgccgtg gtcgcgcagt
     6241 ggctggtgga tgcccggctc gacgaggcgc tggtgcacga catctgtcgc gccctctggc
     6301 accccacggc acgcgccctg ctcgatggcg gccacgcgaa gggccggcag atcaccctcg
     6361 agacggcgct cgacggggcc gccattccgc tgcatcccgg cgccgagcgc tactaccgcg
     6421 aggtcgggct gctgcccccg ggcggctgag cgcgacgttc agaagcgtga gatggccgcc
     6481 acctgcaggc cgaaatgccg ctcgccgtcg cgtgtcgccc ggcgatagct gaagaagtcc
     6541 tccgcctcgg cgcaggtgtc gcggtcgagc cgctcgacag tggcgatccc ggcccgggcc
     6601 aggcggtcga cgcagtagcc ggcgagatcg aaatgacgct gcccggcggc cacggcccga
     6661 aagaagcggc ggctctcggg ctcggccgcg agaaaagccg cctcgaactc ggcacccacc
     6721 tcgtaggagg cgacggcgat gcaggggccg atggcggcga ccgtccggcc cggcacggcc
     6781 ccgagccggc gcatggcttc cacggtcgcc tcgaggatgc cgtccttcgc accacgccag
     6841 ccggcatggg ccgcggcaac gaccccggcc gccggatcgg cgaagaggac cggggcacag
     6901 tcggcggtgg tgacgccgag cacgagcccc ggcgtcgccg tcgccagggc gtcggcctcg
     6961 ggcggtgcca agggatcgaa ggcggcctcg acgatggcgc agcgcgtgcc gtggacctgg
     7021 cgtgcgatca ggagaggatg gtcggcttgc ccgagcgccg ccacggcgcg gcgccggttc
     7081 tccagcgcgt tcgccgtgtc gtcggcgttg cggagcgaga cgttgaggct ggcgaacggc
     7141 ccctcgctga cgccgccccg ccggccgagg aagccgtgcg cgacaccgtc gagccgggcg
     7201 agcgccagag cctcgagctt catggccggg ccctgacggg cgcgttcgcc tcggccgcga
     7261 cgaagccggc cggcgggccg ttgccccagc cgtcgatggt gagcacctgg aagagctcgc
     7321 ccatggcagc aggttccgtc agccgcgcat ggccaaggcg aagcccggtc gcggcggcgg
     7381 ccgaagcccc ggcgagcagg tgcccgaggc gcaggtcgat gccgaggcgc tggaggaaga
     7441 ggccctgcgg caccgggccg aagacggtgg gcccggcggc accggcaaca ttgatcaacg
     7501 gagcgaagtg gacccgcgac gtgagatcga cctcgcccgg ccgggtcagg ggatcgaccc
     7561 ggcgatggcg gctgaccgcc tgcagcgtgc tgccgggggc cggatcgcgc tcgccatagt
     7621 cgacgaccag cgccaggccg ccctggtcgg cgaggcgatg ggcgatcccg gccacggccg
     7681 cctcgcgcgc cgggctgagc tccaggacac tgccgatcgg tgccgcgggc aggcggaggg
     7741 cgagaggcga gggcctggga tcgggtgcga aggcgagccg gccccgggcg tcgaggccga
     7801 gctggatctc gtgccagcca tcctccagcc ggatcagctg gcggatcggc agggcgtcga
     7861 ggaactcgtt ggcgacgacc aggagcggcc ggtcggccgg cacctcgtcg agcgccgcgt
     7921 gccaggcgag accgggaacg gcaccgagcc gctcggcctg ccgccgacgc aggagcgggc
     7981 tcgtctcgac cagatgcacg tcgagcgcgg cgtggaagcc tttgaccccg gccgtcgccc
     8041 gccagagatc ggcgagcagc gtgccgttgc cggggccgag ctcgaccagc cgggccggcg
     8101 ccccccccgc ctgctgccag gcagtggcca gcgcggcgcc gatgagctcg ccgaagagct
     8161 gcgacgcctc gggggccgtg acgaaatcgc cgccggcacc gagcgctgcc cgggacgagt
     8221 agtagccatg cgtcgggtgg ccgagggcga gggccatgaa ggtggcgacg tcgatcgggc
     8281 cgtgcgcctc gatcagggcg acgagctccc gcccgagcgg gctcgccacg tcatcgtcca
     8341 gcccggccgg cgatgcggcg gcgaccgtca cgaggccacc cgtcgcccgc ggtcgcgcca
     8401 gctccaggcg atcagcacga gcccgagcag gatcatcggc aatgacagga gctgccccat
     8461 ggtgatgaag ccgaagagca ggccgagatg cgcatcgggc tcgcggaaca gctcgacgaa
     8521 ggcccgacag aggccgtagc ccaaaaggaa ggtcccaccg agccggccgg cggtggcggg
     8581 atcgcgagcc ccgcgcgcca tgacctgcag cacgatgaag agcaggattc cctcgagcgc
     8641 cgcctcgtag agctggctgg ggtggcgcgg cagcggcccg gcattgggga agaccatgcc
     8701 ccagggcagc tcggtcggcc gcccccagag ctcggcgttg atgaagttgg cgagcctgcc
     8761 gaagaagagc ccgacgggcg tcaccgtggc gacggcgtcg ccgacctcca gggcggggat
     8821 gccgcggctg cgggcgaaga gccagcaggc caccagcacg ccgacgaggc cgccatggaa
     8881 cgacatgccg ccctgccaga cggcgaggat ctgcagcggg ttcgagagat agtagagcgg
     8941 gtgatagaag aggacgaagc cgagccggcc gccgaggatg acgcccagcg tcgcgaagaa
     9001 gagcaggtcg tcgacctgct cgggcgtcat cgtccagccg ggccgggcaa ccatgcgctt
     9061 caggagctgc cagccgccca ggagcccggc gatgtaggcc agcgcgtacc agcggatcgc
     9121 gaaggggccg agctggatca gcaccgggtc gaaggccggg aacgggatcg ccaaaaacga
     9181 caagatcaat ggctccagct aaattaagag gttagcaccc gcgcagctcc cgaggccctg
     9241 atagtggctc ccgcgcgccg cgacaagccg gcccctcggg tgttgccggg cttgttcccc
     9301 ggaccccgat gcccgatatt ccccctaccg gctcgatggc gacgccgtgc tcgatggagg
     9361 tcccgatgca gaccgagaac cgcctgttcg acgatctggc ccgtgttgcg agcggggcca
     9421 tcaacacgct gggcggcgtg cgcgaggaaa tcgagctgcg cgtcaaggag cggctggagc
     9481 ggctcgccgg cgagatggac ctggtcaccc gcgaggagtt cgatgccgtg caggcgatgg
     9541 cggcgaaggc cagggccgag caggaagccc tgaaggcgcg gctcgaccgc ctcgaggcga
     9601 agctcgccgc gctcgagacc gccgatccgg cgccggaggc cagggcgcgc ccgaaacgcc
     9661 ggggggcgga agggcccgac ccggcgaccg aggcgggatc cgccggctga tcgatccccc
     9721 tcccgcccag ccttgtcagc ccccgacgct tttggcaggg tccgatcgga tggtcaggcg
     9781 atccgctcgc ttgccatccg gcaagcccag ccgcccggtc gctccatgcg atcggcggcg
     9841 gcacggactg ccccgccttt ggagtgcacc ttcgtgactt cccttctttc cgaattcgcc
     9901 gttccgcccc gcaacccgct cgagctggtc gagaagatgg ccgtcgccaa cgattggccg
     9961 ttccatcgcc agagcgacga ggagctggcc gccgagatca gcggccagtg gtgccactac
    10021 aagctgtggt tcgcctggca cccggagatg ggcgtgctgc acctgtcctg cgccctcgac
    10081 atgaaggtgc cggccaagaa gcggccggac ttctatcacg tcctcgcgat cgccaacgag
    10141 aagctgtggg tcgggcattt cgagatctgg cccgaagagg acctgccggt cttccggcac
    10201 gcctcgctgt tccgcgaggg ctcgaccgcc agcgcctccc tcatcgagga tctggtggag
    10261 atcgcgctca acgagtgcga gcgcttctac ccggccttcc agttcgtcct ctggggtggc
    10321 aaggagccgc aggaggcgat cctggccgcc atgctggaaa ccgaaggcga agcctgatgg
    10381 cgaccatccc gggcccgctg ctcctggtcg gtggtggccg gatgggcgag gcgctgctcg
    10441 gcggctggct gcggcagggc ctggcttccg gctccgccac ggtgatcgag cccgatcccg
    10501 gccgcgccgc ggcgctgaca gcgcagggtc tcgccgtggt gccttctgcc gccgacctgc
    10561 cgcccggctt tcgggcccga atcctcgtcc tcgcggtgaa gccgcaggtc atggcggacg
    10621 tgctgccggc gttgcccggc catctggcgc ccggcggcct cgtgctctcc atcgccgcgg
    10681 gcaagccgct cgccgtgttc gaggccgcgt tcggcgccgt cacgccgatc gtgcgggtga
    10741 tgccgaacac cccggcggcg atcggccggg gcatctccgc cctcttcgcc aatccggcga
    10801 ccgacgcagc gaaccgggcg ctggccgagc ggctgatgca ggcggtgggg gaaaccctct
    10861 ggctcgacgc cgaggcggag atgcacgccg tgaccgccct ttccggcggc ggccccgcct
    10921 atgtctgcct cctggtcgag gcgctggccg cggccggcga gcgcctgggc ctggcaccgg
    10981 agctcgccat gcgcctcgcc cgggccaccg tcgccggctc cggtgccctg ctcgaatcga
    11041 gcgatgcgac ggcggcagcg ctccgcgagg ccgtgaccag cccgggcggc acgacacggg
    11101 cggccctgga cgtgctgatg ccggcggacg ggggaaccga ggccgggctg agcggtctgg
    11161 tcggcgaggc cctcgtccgg gcggcggagc gatcgcgcca gctcgcctga ggcgaccggc
    11221 tcgggagagc gtgatggagg caggatgagc aggaaggcca aggcccagcc acgaccggac
    11281 ctcctcgagg cggccttcgc cctgatcgcc gagaagggct ggcacggctt ctcggctctc
    11341 gcccttgcgg agcggaccgg cgcctcgctc gacgatatcc acgacctgct gccgacgccg
    11401 gccgccctcg tcggtcggct cggggagcgg ctcgaccggg cgatgctggc agcaccgccg
    11461 gacgagctgc tcgagctgag cccgcgcgag cggctgttcg agctcctgat gcgccgcctc
    11521 gacgcgctcg cccccttcaa gccggggctc gcccggctcc gcgtggaagc gcgggccgag
    11581 gtcgagcttg cgctcgccac gctcgcgagc ctcgaccgga tggccgcctg gctgctcgat
    11641 ctcgccgggc tgccgggccg tggcctgcgg ggccgcctcg cccggcgggc gcttatgctg
    11701 gcctatggcc gcgtcctcgc cgtgtggctc gcggacgaca cgccggacca ggacgacacg
    11761 ctcgcggagc tcgacaagcg cctcgaccag ctcgagcgcc tcgcacgcct cggcgaccgg
    11821 ctgaccgccg ggctcgggcc acgaccacgc gccgcctgag ccgaaggcgt ctcttcggca
    11881 aacgcttcct taaccattga gcggtagaaa cgatgcgccg cgtgcgtcga gtcgcgcgtc
    11941 ggcggtgccg agcggcaccc cttgacgcac cctgaacgac cgagctggag cagcgttccc
    12001 atggccctga cggcaaccac caacaccacc gccaacacgg cacttcgcta tctcagtcag
    12061 aacaacaaca tggccgcgag ctcgctggcc aagctgtcga gcggctcgcg catcgtccgc
    12121 gcctccgacg acgccgcctc gctcgccatc ggcaccaaga tcaaggccga tgtcaccgcc
    12181 ctgaagcagg cccaggtcaa tgccggccag gcttcctccc tcctccaggt cgccgatggc
    12241 ggcctctcgc aggtggtcga catcctgatg cggatgaagg cgctggccgt ccaggcgcag
    12301 tcgggctcgg tgagcgacaa cgagcgtggg ttcctcgaca aggagttcca ggcgctctcc
    12361 ggccagatcg acgccatcgc cgcccagacc aagttcaacg gcaccgagct tttgagcggc
    12421 aacatcgcca ccgcgttctc cgccatcggc acggccatcg acggctcggg ggcggccgag
    12481 ctcaagctca ccggcgggtc cgaggccggc gcctacaccg tcgcctacga cgatgccacc
    12541 ggtgtcctca ccgtcaccca gggcaccggc gtgggcgccg aatcgcagtc ggtcaccctc
    12601 gacaacaccg acaccgcctt caccggcagc gtcgccttcg accagttcgg cctcgaggtc
    12661 gccttcaccg ccttcgatct cgacaccgtg gccatcgccg ccaacaacac ggcgacggtg
    12721 acctccggtg ccatgagctt ccaggtcggc gtcagctcct ccgacaccat cgacgtcacc
    12781 atcgccgaca tccgcacgac ggcgctgggt gacgggacca acacgctgag cgacgacggc
    12841 agcggcagcc ccgccagcat cggcacggcg gcgaaagcgg ccggggccgt cgacatcatc
    12901 gatggcgccc tgtcgcagat cagcgagggc cgcgccgatg tgggtgccct gatctcgcgc
    12961 ttcgagttcg ccgcggcgaa cgtggccacg acgatcgaga acctcgactc ggcacgctcg
    13021 accctgctcg acgtcgacat ggcggcggag atgagcaact tctcctccaa gcaggtcctc
    13081 gtgcaggcct cggtggcgat gctcgcccag gccaaccaga tgccccagca gctcctgaag
    13141 ctgctcggct gagcctgtcg cccggcatga ccgggccatt ccttctgcgt tcggcagcga
    13201 ggccggcccc cccttgggcc ggcctttgtc gttcgaggcg gcggaagtcg acggattttg
    13261 ccggggtcat gcacccggat gcgctttccg gccccgccat cgtcctcctc ccccgcccgg
    13321 cacccgtcgc gttcagcccc tgttaatctc catgggctag cttgtcccta caccggcaca
    13381 tcggtgtcga tcggcagata gccggtcgtc aacgggccat ccggcccctg cttcatgtag
    13441 aaggacactc cccatggctc tcacggccac caccaacacc accgccaaca cggcccttcg
    13501 ctatctcagc cagaacaacg ccgcggcgtc gagctcgctg gccaagctgt cgagcggctc
    13561 gcgcatcgtg aaggcgtccg acgacgccgc ctcgctcgcc atcggcacca agatcaaggc
    13621 cgatgtcacc gccctgaagc aggcccaggt caatgccggc caggcttcct ccctcctcca
    13681 ggtcgccgat ggcggcctct cgcaggtggt cgacatcctg atgcggatga aggcgctggc
    13741 cgtccaggcg cagtcgggct cggtgagcga caacgagcgt gggttcctcg acaaggagtt
    13801 ccaggcgctc tccggccaga tcgacgccat cgccgcccag accaagttca acggcaccga
    13861 gcttttgagc ggcaacatcg ccaccgcgtt ctccgccatc ggcacggcca tcgacggctc
    13921 gggggcggcc gagctcaagc tcaccggcgg gtccgaggcc ggcgcctaca ccgtcgccta
    13981 cgacgatgcc accggtgtcc tcaccgtcac ccagggcacc ggcgtgggcg ccgaatcgca
    14041 gtcggtcacc ctcgacaaca ccgacaccgc cttcaccggc agcgtcgcct tcgaccagtt
    14101 cggcctcgag gtcgccttca ccgccttcga tctcgacacc gtggccatcg ccgccaacaa
    14161 cacggcgacg gtgacctccg gtgccatgag cttccaggtc ggcgtcagct cctccgacac
    14221 catcgacgtc accatcgccg acatccgcac gacggcgctg ggtgacggga ccaacacgct
    14281 gagcgacgac ggcagcggca gccccgccag catcggcacg gcggcgaaag cggccggggc
    14341 cgtcgacatc atcgatggcg ccctgtcgca gatcagcgag ggccgcgccg atgtgggtgc
    14401 cctgatctcg cgcttcgagt tcgccgcggc gaacgtggcc acgacgatcg agaacctcga
    14461 ctcggcacgc tcgaccctgc tcgacgtcga catggcggcg gagatgagca acttctcctc
    14521 caagcaggtc ctcgtgcagg cctcggtggc gatgctcgcc caggccaacc agatgcccca
    14581 gcagctcctg cggctgctca actaggtcga gcgagccccg ggccggcgcc agccggcccg
    14641 gggcgacgtt tcgtgaagcg ggcttaaccg atgctcaacc ctttgccccc tatgctccag
    14701 gcgagcggcg ttccggcgcc ggctggcagg accggtcagg cgccgacggc tcctaccacc
    14761 aaggtgcccg ccgaagccgc cgcaacgtcg ctcgagcggg tcgtccagca ggcggcacgg
    14821 gatctctttc ccggccggga ggtcgcggtg gaaagcttct tcgacaaggc cagcggtcgc
    14881 ttcgtgcatc gcatcgcgga tggcgagagc ggcgagatcc tggcccagac gccatccgac
    14941 tcgctgctgc gcttcttcgc gagcggccgt gcgccgctcg gtgggccgct cctgcgcctc
    15001 gaggcctgag cggcgggaga cgaaccatgg cctcctcgat cagcttcggc agcctttcga
    15061 ccagcagcgg ctcgccgcgc ctctccggca cgagctcgaa gctcgacacc gaagccgtgc
    15121 tgcaggccac ctacgaggcg aagcgcctgc cggccgttcg gctcgggaac aagaccacca
    15181 ccaacgagac gaagatcgcc gcctacgaga gtatgcaggg cctgctcgag agcctgcgct
    15241 ccgccgccaa cggcctgcgc aacccgccgg gctttctcgg tgcacgcgac aatgccttcg
    15301 agaccaagca ggccttcctc ggctcgagca ccacgaccgc accgggcgag atcgtcggtg
    15361 tcgccctcgc caacacggcc caggcgggga gcttcgacct gcgcgtcacg cagctcgcca
    15421 ccgccgagaa gctcgccacc ccgggcgctg ccgccgccaa tgccacgctc gccgatgcct
    15481 ggaatgccgg cgatccgttc agcggcacga tcgagctcgg cgtcgccggc ggacccacgg
    15541 cggtgatcga cctcgacggc gatctcggcc tctacgacct gcgcgaccgg atcaatgccg
    15601 tgcgctcgac caccggtgtc ggcgccagtg tcctcaaggt cgccgacaac gacttccgcc
    15661 tcgtcctcgc cgccgaggag accggcaagg cgatcacgct caacgatacg agcgggctca
    15721 ccggcggatt ctcgaccacg acgctgcaga cggcgcagcc ggcgatcttc gagatcgacg
    15781 gcgtttcggt cacccgcacc tcgaaccagg tcgacgacct catgggtggc gtgacgatca
    15841 acctcttcaa ggccgagccc ggcaccacgg ttgccgtgtc cgtcgagccg agcctcggcg
    15901 acatcaagag ccgcatcgtg gcgttcgtgg aagccttcaa cgcgctgcgc gggttcgtca
    15961 acgagcagtc ggcggtcgac gcgtcgggtg ggccggcggc ggacgcggtg ctgttcggcg
    16021 accggacgat gcgcggcatc atgaccgggc tcggcggcat cgtcggcggc tcggtcgccg
    16081 gcctcgatgc cgacgcccta ggctcgtttc gcgccatcgg catcgagctc gacgccacga
    16141 accagctcga gatcgacgac gccaagctcg acaatgccct cctgacgaag ctcgacgacg
    16201 tccgcgacat cttcgagttc cgctaccggt cgtcctcctc cgagctcgcc atcttcgccc
    16261 ggagcaatgc gctcgccgcg accacgatgc agctcgagat caccgatgcc gacaatgacg
    16321 gcacgcccga ggcggtgacg atcaacggcc aggctgccga gatcgccggc tcgaccatca
    16381 agggtgccgc cggcaccgcc ttcgaggggc tggagttcat ctggaccggc cggggcagca
    16441 cggtgatcga cgtcgaggcg agccagggcc tggccgaccg ggtcttcaac ttcgtcaagc
    16501 cggcggtcga tccgctcgat ggctcgatcc cacgcgccat cgattcgctg cgcgacctca
    16561 acggcaatta tgcgaagcag atggagcgga tcgaggagcg cgccacccgc gcccgcgacc
    16621 tcctggtcga gcggttctcg gcgatggagg cggccctctc cctcgcgaac gccatgctcg
    16681 cccagatccg ggcccagatc gatgccatga cccaggattc ctaggcgatc cggaccgcga
    16741 tggcgccctc accgaccctt gcatgaatgg ccgtgccggc aggcgaccgg cccctgcgaa
    16801 atacacccct ggagatgagc ccgatgcagc aacgtgcccg cgccatgacg gcgtatcaga
    16861 ccgccgccct gacggtcacg ccagcccggg ccatcgtcat gctctacgat ggcgcggtgc
    16921 agcgcctgca gcaggccggc cgggcaatca ccgagggccg catcgaggag cgctacaggc
    16981 tcgtcggcaa ggcgcacgcc atcgtccacg ggctgcagtg ccagctggat ttcgcggccg
    17041 gtggcgaggt ggcgctcctg ctcgaccgct actacgccta tctcctgcac cgcatgacgc
    17101 agatcaacgt ccgcaacgat ccggcgatct gcgacgaggt catcgcccgc ctgcgcgaca
    17161 tgcgcgcgag ctgggccgcg atcgccgaga ccggcaacgc cgcccctgca ccgccaccgg
    17221 ttcaggccga ccaccgaccc attgtccagc tcggctgaag ccgagcctcg cagcgtctgg
    17281 tggatcaccc agcctgggga cgggcccggc actcggcgca ccttcaccat gccggcgact
    17341 cgacgatggc cagcagctcc ggaccggcgc gacacgcccc ggccgaccgt cgtcgaacgg
    17401 gcgcccaccg gcctaataaa gggtttcttt tttagttggc atagcccggg ttcgccagtt
    17461 tgttgcggca ctcctcggtc gagaacccgt ggaggagctt gccgatggcg tcgtgcgggg
    17521 catcgacggt ccgggcaccg gccttgcgca agagatgctc gagcttggcg aagacctgtt
    17581 cgcctccgcc gcctgctggc ggcgaccctg tcatggttcg gatcaaacgc cttctggcct
    17641 ttgatggctt gaggtcggga ctgtagggcg gcaggtggac gagttgggca ccccgggcct
    17701 cgacggctgc cctgatgccg gcgaccttgt ggctgccgag attgtcgagg atgacgatgt
    17761 cgccgggcgc gagcgtcggc gccagcgcct gctctgtcca tcatccgtca ggagacggat
    17821 gatccgatcc atggaatgga cgccgccatg aggcggcgga ggggctttga atacggcgca
    17881 gtcgatcggt ccgtcgaaga cgcagggtgc ggtgatccga tcatgcctga gggtggcgcg
    17941 acgccgtcga cccgttggcc cttcggcccg cgcccgcagc gcggcgccat gttggtggat
    18001 gtccaggttt cgtcgacaaa gacgaggctt gccgggttca gcccgcgctg ccggccgacc
    18061 cacgcctggc cggccgtggc gatgtcctcg cgcgactgct cggcggctct cggcgtcttt
    18121 ttttcgcgtc aggccgagcc tttcgtggca cctggcaagg gctgccgggc tcgtcttcgt
    18181 gccgcgttcc gacagccgtg attgcagctc ggcgatggtg atgtccgatc gctccgcaat
    18241 ccagagccgc agatcctcct catgcgcctt cagcggcgaa acctcgtagc cgccaagccg
    18301 ggccggtgac acctcgccag tcgcctggac ccgctgcagc agcttgatca cgaagctgcc
    18361 ggtgaccctt aaaaactcgg cggcctgccg gcgcgacgca gcgttctcga ccgtctcgac
    18421 aactctcagc cgcagatcat ccgaataggg acgtggcatc catggctggc ctcctcgtcc
    18481 agccagcagc tcgaatcaca tctatcaaag ctcgggactc cctgacatga ttccgtcttt
    18541 tgggaatttg ctctagaggc cgtccatatg ctgaggcatc ttcactccac ctccccgccg
    18601 ctctcgaccc agcccttgaa ggcgccgaga tcgctgacgt cctggtagcc gagctcctgc
    18661 agcgtcttgc cgaccagggt cgagcggccg ccggaggcgc cgtagacgat cacggtcttc
    18721 tccgggctca gccgctcgtc gcgacagggc gaagcgggat cggcgcggaa ctcgatcatg
    18781 ccgcgcgaga tgttgacggc acccctgacc ttgcccgagg ccgccacctc cggcgcgtcg
    18841 cggacgtcgc cgacgagggt gcccgggtcg gcgagcaggg cccgcgcctc ggccggcgtg
    18901 atcggcggca cggcggtgtt ggcttcggcg agcatgtcct cgacagtcct cggcatcaac
    18961 gtccctccag gctggggtcg cgccctcggc gaccgcccga cgtcgatggc gcagggctca
    19021 gccgatggcg ctggccgccg cgtcgagctg gtcgatgacg gccttcgcca gctccgtggt
    19081 cgtgtgccgg ccgcccaggt cccggggcac cgcgccgccg gcgaaggcga ggtcgacggc
    19141 acgctcgagc gtggcgccgg ccgtcaccat gctctcctgg ccgtgccgct cgccgagcca
    19201 gtcgagcatc atggcggtcg agagcagcat ggcggtcggg ttggcgatgc cctggccggc
    19261 gatgtcgggg gcggagccgt ggctcggctg gaagagcgcg tgctcgaggc cgatctcggc
    19321 gcagggggcg aggcccatgc cgccgacgag gccgccgccg aggtcggaga tgatgtcgcc
    19381 gaacatgttc tccatcacca tcacgtcgaa ctcccagggc ttgcgcagga ggtcgagggc
    19441 cgtggcgtcg acatagtggt ggtcggcccg gatgtcggga aagcccttcg cccgctcgtc
    19501 gaagatcttg cggaagaagg ccatggcgac gaagacgttg gccttgtcga cgcaggtcag
    19561 ccggccgggc ctgcccttgg ccttgcgccg ctcggcgagg cggaacgaga aatcgaagag
    19621 ccgctcgcag ttgtcgcggg tgatgaccat ggtctcgcgc gcgatccggt cgtcctcgac
    19681 atagccgaca ccgcgcgacg cgaagaggcc ctccatcgat tcccggatga tgacgaagtc
    19741 gatctcggcc gcccgcgggt gggcgaggat ggccggggtg ttgggcaatg cccggaccgg
    19801 gcggacaccg gcgaagaggc cgaactcccg gcgcatgtcg agatgcgggg cgatctcggt
    19861 gccgtcgggg aagcggacgt ccggcaggcc catggcgccg aagaggatgg cgtcggccga
    19921 gcggcaggcc tccatcaccc cgggggcgag gtccctgccg gtttcgcggt agtaggcggc
    19981 accgccatcg tggtgctgga gcgcgagacc gaggccgaag cggctttcga gaacctcgag
    20041 aacctcgagc gccacggcca tgacctcgcg gccgatgccg tcaccgggca ggaccgccac
    20101 ctggaattca ttcatatcga gactctgccg gatgggccgc atccgcccgc aggacgagcg
    20161 gaggcaaggc ggggactgac aagcgataca tcaacggata cattaccgtg tgcgaccggt
    20221 caatcaggca gcgcccggta gtgaccggac gtggcgatgc gtgctaggga ccgggcattg
    20281 ttggctcggg aaggtcaggc ggcacagatg acggcaacga tcgacacgtc gcccaaaatc
    20341 gcggacgaga tcgtttgcac cacctgctac atgtgcgcct gccgctgcgg catccgggtg
    20401 cacaagctgg acggcgagat ccgctacatc gacggcaatc ccgagcatcc ggtcaaccgg
    20461 ggcgtgctct gcgccaaggg ctccgccggg atcatgcacc acaactcgcc cgcacggctg
    20521 accaagcctt tgaagcgggt cggcgagcgc ggcgagggca ggttcgaggc gatcgagtgg
    20581 gacgaggcgc tggccatcgc cgagggctgg ctgcgcgggg tgcgagagac cgaccccagg
    20641 cagctcgcct tcttcaccgg ccgcgaccag agccaggcct tgaccggctt ctgggccagc
    20701 cagttcggca cctgcaacta cgcggcccat ggcggcttct gctcggtcaa catggcggcc
    20761 gccggcctct acacgatcgg cggctcgttc tgggagttcg gcgagccgga tttcgagcac
    20821 gcccgctact tcctgatctt cggcgtggcc gaggatcacg acagcaatcc cttgaagctc
    20881 ggcatggcgg agctgaagaa gcgcggggcg aagatcgtct cggtcaaccc gatccgcacc
    20941 ggctactcgg ccatcgccga cgagtggatc ggcatccgcc cgggcacgga cgggctcttc
    21001 gtcatgagcc tgatgcacga gctgatcaat gccgggcgga tcgacgtcga gtatctgcgg
    21061 cgctactcca acgccacggc cctcgtgatc gacgcacccg gcgacgccga tgacgggctg
    21121 ctcgcccggg acggcgacgg gcggctcctg gtcgggctgc agggcaaggc gacggtcgcc
    21181 ccgacggggc ccgccgtcca ggccgccctc cagggccgct acacgctggc cgacgggcgc
    21241 gccgccgtgc cggtcttcga gctgctggcc cggcgctatg cggcagccga gtacagtccc
    21301 gacgcggtgg ccgaacggtg cggcattccg gccgccacca tccggcgcat cgccgccgag
    21361 ctggcggcgg ccgccttcga cgcgcccgtg gtgctggagc gcccctggac cgacacgaac
    21421 ggcatgcggc acgagcggat gatcgggcgg ccggtggcga tgcatgccat gcggggcatc
    21481 tcggcgcaca gcaacggctt ccatacctgc cgcgccatcc atctgctgca ggtcctgctc
    21541 ggcaccatcg acacgcccgg cgggtttcgc ttcaagaccc ccttcccccg cccgacgccg
    21601 cccgggccgc agccggccgg ccgggcggac cagatggcgc cgggcaagcc catggcggga
    21661 ccgccgctcg gcttcccgcg cgggccggcg gacctgatcc tcgacgaggc gggcgggccc
    21721 cggcgcatcg acaaggcctt ctcctgggac gcgccgatct cggcgcacgg catgatgcac
    21781 agcgtcatcg gcaatgcgtt ccgtggcgat ccctacagga tcgacgtgct cttcatgttc
    21841 atggccaaca tggcctggaa ctcggccatg aacacgaccg agaccatggc catgctcgag
    21901 gcgaagaacg cggatggcag ccatgtcatc ccgaagatca tctatgccga cgccttctac
    21961 tccgagacgg tggcctatgc cgacctcgtg ctgccggaca cgacctatct cgagcgccac
    22021 gactgcatct cgctgctcga ccggccgatc ggctcggccg atgcggtggc cgacgcgatc
    22081 cgccagccgg tcgaggtccc ggaccgggac gtgcgcccct tccaggacgt gctgatcgac
    22141 cttggtgcac ggttgggcct gccggccttc gtcgacgcga gcggtgcgcc ccgctttccc
    22201 ggcggctatg ccgactacat ggtcaaccac gagaggaagc ccggcgtcgg ctcgctcgcc
    22261 ggctggcgcg gacccaatgg cgaccaggcc ggcaaggggg ccgtcaaccc ggaccagctc
    22321 gagcgctaca aggcgaacgg ctgcttcttc agcctgccga tccccgagga agccacctac
    22381 ttcaagcacg ccaacgcggc ctacctgaag ctcgcggtcg agctcgggct gatcgacgag
    22441 gcggcgccga tcaccatgca gctctactgc gagcccctgc agcgctttcg cctcgccgcc
    22501 cggggccacg gggccttgca gccgccggcg agcaagcggg agcggatcga gacctatttc
    22561 gatccgctgc ccatctggta catgcccttc gacgaggcgg cggtcgacgc ccaggccttc
    22621 cccctccacg cggtgacgca gcggccgatg ttcatgtatc acagctgggg cgggcagaat
    22681 ccctggctgc gccagatcct cgatcgcaac gccctctacc tcaatcgccg gaccgccgcc
    22741 gggctcggca tcgaagaggg cgaccgggtc gccgtcacca gccggcacgg gcgggtcgag
    22801 ggccgggcga agctgatgga cggcgtccat ccctccaccg tctggacctg gaacgccatc
    22861 ggcaagcgcc gcggcgcctg ggggctccag ggcgacattc ccgaagcgag cgaggggttc
    22921 ctcttgaact cgctgatcga cgacctcctg cctgagcagg acggcggcta tcgctacgcc
    22981 aatgccgacc ccatcaccgg ccaggcggcc tggtacgacc tcagggtgag ggtcgagaag
    23041 ctcggtgcgg aagcgcgagc gccggagccg gcggcgctcg cccgcccgcc cggcgccatg
    23101 ccggtgccgg agacgttgag ctaccgggtg cggggcggcg catgaccagc ctgcccgcca
    23161 acccctcggc caagcggctc ggcctcgtca tcgatctcga cacctgcgtc ggctgccagg
    23221 cctgcgtcgt ccactgcaag gaatggaaca ccagcagcgg cgcctcggcg ctgagcgaca
    23281 ccgaccccta cggcgaggcg ccgagcggcg cctggctgaa ccgggtgcac ggcttcgagc
    23341 tggacgacag cgcctctggc ggctgcggca gcaccgtgca cttcccgcgc tcctgcctgc
    23401 attgcgagaa cgccgcctgc gtcaccgtct gcccgaccgg cgcctcctac aagcgcgccg
    23461 aggacggcat cgtcctggtc gacgaatcga agtgcatcgg ctgcggcctc tgcgcctggg
    23521 cctgccccta cggggcgcgc gagatggacc gggtcgcccg ggtgatgaag aagtgcaccc
    23581 tctgcgtcga ccgcatctac aacgagaacc tgccggtgga ggagcgcgag ccggcctgcg
    23641 tgcgcacctg cccggccagg gcgcgccatt tcggcgatct cggcgacccc gagagcgacg
    23701 tctcgctgct ggtcgccgag cgcggcggcg tggcgctgat gccggagctc gattatcgcc
    23761 cgaccaaccg ctacctgccg ccgcgtcggc cggtcccggc ggccgaccgg ccggcggcgc
    23821 tggagccggc cccgggcggc ttcctcggct ggctcgaccg gctgctcgcc gaccaaccgt
    23881 aggggcgcag acgtgcatcc agcaccctcg atcatcttct tcaccgcggc ctctggtgcc
    23941 ggctacggcc tcctggtgtt cgccgccctg gccggactca tcggcctggt gccggtcggc
    24001 agcgccagcg ggttcgccct cctcctgccg gcgctgctgc tggtcgccgc cgggctcctc
    24061 tcctccgccg gccatctcgg ccacccggag cgggcctggc gggccttcag ccagtggcgg
    24121 tcttcgtggc tgtcacgcga gggcgtcgcc gccgtcgtca gcttcgtgcc ggcgctcggc
    24181 ctcgcctggg cctgggcgct gaacggccgg ctggcgccgc tcctggcgct ggcgaccatc
    24241 ctgatggcgg cgctcaccgt ctattgcacg gccatgatct acgcctcgct gaagccgatc
    24301 ccgcgctggc accaccggct cgtcccgccg gtctatctgg gcctgggtgc cgcgagcggc
    24361 agcgccgtcg gcatcgccgc cgcggcctgg acgggtgctt cggtgctgcc gctggccccg
    24421 ctcgtggcct tggcacttct cgtcggctgg gggctcaagt ggcgctactg gcagtcggtg
    24481 gacacgcagc ccgccaccgc caccacggcg gacgcggtgg ccctcggcga tcggggcacg
    24541 gtccggctgg tcgagccgcc gcataccgag cgcaactaca tcctggacga gatgggcttt
    24601 cggatcgccc gcaagcacgg cgccaagctc aggcgccacg cgctcatcgc cggcctcgcc
    24661 ttgccgctcg tcgccctcgc cctggccctg ctcctgccgc aagtcccggc ggcgctgctc
    24721 ctgagcatcg gcgccgggct cgcccttgtc ggcgtggcga tcgagcgctg gctcttcttc
    24781 gccgaggccg agcatctggt gatgctctac tacggca
//
LOCUS       DAOTFI010000033        24806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2346_length_24806_cov_7.019676, whole genome shotgun sequence.
ACCESSION   DAOTFI010000033 DAOTFI010000000
VERSION     DAOTFI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 24806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24806
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2346_length_24806_cov_7.019676"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="P5558_04950"
     CDS             complement(<1..238)
                     /locus_tag="P5558_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY23688.1"
                     /translation="MDYDPVLLLPLLLVPLFIILISLKIMREYERAVVFLLGRFWKVK
                     GPGLILVVPGLQTMVRVDLRTRVLDVPSQDVISRD"
     gene            complement(247..1557)
                     /locus_tag="P5558_04955"
     CDS             complement(247..1557)
                     /locus_tag="P5558_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRY23689.1"
                     /translation="MKRSSALASLLFLLGMLLPLLGMPASAEATTVQVVPVKGPIGPA
                     TSDFLVRAIEAAAADGADLVLLEIDTPGGLDTSMRTMVEAILDAPLPVIGYVAPPGAR
                     AASAGTFILYATHLAAMAPGTNLGAATPVQIGGDAGERSAHATKAMEDAAALLRSLAT
                     MRDRDPKFAESAVREAKSLTAEEALKAGVIELIAPDRAELLRRVDGRMVELKSGPALI
                     DAADPAIVESQPDWRYQLLAIITDPNVAAILMLVGIYGIIFELYSPGLVGPGLVGAIC
                     LLLGLYALQVLPVSFAGLALLLLGIILVIAEAFVPSFGALGLGGIVAFVAGAVLLVDT
                     DVPGFGVSPWLIGAVAIVTSGIFLLALGLALRSHRRPVVTGAEAVLAGRGTVVDWADG
                     EGHVRLEGEIWAARGPDDLASDTPVTVEARDGLVLVVAPQPPGG"
     gene            1646..2770
                     /locus_tag="P5558_04960"
     CDS             1646..2770
                     /locus_tag="P5558_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY23690.1"
                     /translation="MTTLTLTSWNIEHFSRLLDGNAGEAERLAAIAAEIRAIDPHVLL
                     IVEGPASLPALEAWVAAAEPHGLGGGWRVATIPGTNEALEARPDDPRRALAGLYAMQG
                     NRISGGQWLWFLVKDGLFQESRATLLAPLVWQDLTGQRDWPVHRWGELISRRHAHWRH
                     PQVLLLQMGGVEIEVVGAHLKSKINHERAFDEAGELTHDYVDEALRARIQLATEAYDI
                     RRYIDGRFAQTPKPRILVAGDFNDGPGRGFFEREYLYFDLVGNLQGDVFAADKFLNHA
                     LFDFPGDLRWTTAFDDRVERWSRTRDGTPPLPAEPYDATRFQLIDHILFTQALVGEGA
                     CPRIRGGAGRVEHTVHQRINAGLGRRRTSDHVPVSVELTL"
     gene            complement(2760..3647)
                     /locus_tag="P5558_04965"
     CDS             complement(2760..3647)
                     /locus_tag="P5558_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY23691.1"
                     /translation="MKLFPGFQGHRLSTAETGIFARTKGEGPPLLLLHGYPQTHAMWH
                     RVAPALAETFTVVCADLRGYGDSGKPVGDGTHRAMSKRAMAADLVEAMRMLGFDRFMV
                     AGHDRGGRVTHRMCLDHPKVVTRAAVLDIVPTRTIFAATNKAIAEGYYHWFFLSRPAD
                     FPERLIGADPIYYLHQKLGGWSAGKSSTFVHPDAMAEYERCFASPATIHASCEDYRAA
                     ATIDLEHDEADEDARIQCPLLVLWGRHGLMEKHFDVLATWREKAAGEVTGEALDCGHY
                     LPEEAPEATTEKLEAFFRA"
     gene            complement(3682..5070)
                     /locus_tag="P5558_04970"
     CDS             complement(3682..5070)
                     /locus_tag="P5558_04970"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY23692.1"
                     /translation="MRGIVQRLASGAARERAGRVLLGPPLPEAMPQRVADAIGREQAR
                     SEVLVSLIQLGAIVLFAVLYSLTPKAFPPDVPFEPVPIALVLYALFTFWRLGLALRQR
                     LTRPILAVSVVVDIALLMVTIWSFHLQYQAPPALYLKAPTLMYVFILIALRTLRFEPA
                     FVLLAGISAALGWLALVAYAVLAGGGPAGETMITRDFAEYMMSYSILIGAEVDKIVSI
                     LVVTAILALALHRARKLLVRAAVEGQAASDLKRFFAPEIAGRITGSDAELVPGRAELR
                     EAAIMFIDLRGFTGLGERLAPADVMQLLGEYQALMTRAVRAAGGSIDKFMGDGILASF
                     GAVRPSDSAAAEALAAIEAVLETGQRWSGDRARRNLPAPAVAAAITTGTVMFGTVGDA
                     QRLEYTVLGEPVNLAAKLEKHTKVERSAGIVMARTFDSALGQGFTPRLHWELRPARTV
                     DGLGRAIDLRVC"
     gene            5069..5341
                     /locus_tag="P5558_04975"
     CDS             5069..5341
                     /locus_tag="P5558_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23693.1"
                     /translation="MGGLRNCANEVYAERRSAPTGRVLAAHHVTLSRAAWTPVNEGNV
                     TPAHPGRQCCIAKTGMVTGHESRHIPDRWEQRVDDALQYEFEGPVP"
     gene            5338..5586
                     /locus_tag="P5558_04980"
     CDS             5338..5586
                     /locus_tag="P5558_04980"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRY23694.1"
                     /translation="MNNQMTLPVGGVAGAWRGESRDTSRFGQLIAALRRQLAVHRVYR
                     RTYDELMTLTDRELDDIGVARCDIQRVARDTAREVVPA"
     gene            5679..7109
                     /locus_tag="P5558_04985"
     CDS             5679..7109
                     /locus_tag="P5558_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRY23695.1"
                     /translation="MNLEQFRQEAHGLVDWMVAYLSTIERRPVRSPATPGSIAAQLPA
                     AAPEGPEPFEAIFRDFERIVMPGMTHWQHPRFFAYFPANSSPPSVLAEMLTATLAAQC
                     MLWQTSPAASEMEGRVLDWLRQMLGLDAGLAGVIQDSASSATLCAVLVARERATGWQA
                     NEAGLGACPPLAVYTSDQAHSSVEKAVRIAGLGRSALRLVPSDERFAMRADALAGMIE
                     ADLRAGVKPACVVATLGATGTGGIDPLAAIGRLCRAHDIYLHVDAAWAGSALLLPETR
                     WMIDGIEQADSFVTNPHKWLRTNFDCSVHYVRRPDELVRTLSVMPAYLTGPGTGQAVD
                     YRDWGIPLGRRFRALKLWFVIRSYGISGLQAMLRDHIAWTRELAAWIEATPGFELVSP
                     PVLGLLSFRCRPSGLDAEGLDALNRRLVERINDDGRIYLTQNIVRGRFALRFSIGQAT
                     TERRHVEEGWAVVRELAEQVVRTRNV"
     gene            complement(7294..9051)
                     /locus_tag="P5558_04990"
     CDS             complement(7294..9051)
                     /locus_tag="P5558_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23696.1"
                     /translation="MGEEPIPDEAVLAALARVLASAELEGSPRLQAFLRHIVEARLAG
                     NERAMRAYAIGVDVFERPADFDPQAESLVRVEAGRLRQKLAAYYAGEGKADPVRITLP
                     KGGYVPRFEAARTGAPAPARTAARAPALTRAKPAPSHRRPYRVAFLTAALIAIAIGIA
                     AHLGLERGSPTAPVEVNGAADLARQTPAIGVLPFEALDDAPGTTTVAGGLTALVTAEL
                     THFRQFFVLARRSALALAEAGGDPIRAAAEAGLDYVVDGTVRRDDEGFVVTASLISTE
                     DAGVLWTRTFTGPHAGNGALALESEIAAAIVTAVAQPWGLVNRQLSRAADQADLPASL
                     ASYACILAADHYYATYAPADYTAAKTCLERAVRTEPDYAHAWAYLAYLRLEALRYGYT
                     HDPAGQELAAALEAARKAVELEPENAVAQRSLAAVLFTAGDMDGFRRHADLALELNPN
                     DSDALADLGGKIAYSGDWERGLAMRARAIELNPAHPASYRIPFVIDAYRRGDYAAARG
                     ELEQSDLPDFLMTRLLRAAVYGRLGLQRQAAQAVQDLLELAPDAADRAGEYLGYWQLP
                     PDLVEDLLGGLQSAGLKAA"
     gene            9411..9686
                     /locus_tag="P5558_04995"
     CDS             9411..9686
                     /locus_tag="P5558_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23697.1"
                     /translation="MDPMDPRARSFSLVGAFLAAARPSPWLFRRWMTAAHEAAAEILG
                     DPDADVFAYNDAAEIDQEGVMALLDEIDARVTPLVDVDLPTPPGSGR"
     gene            9683..10057
                     /locus_tag="P5558_05000"
     CDS             9683..10057
                     /locus_tag="P5558_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23698.1"
                     /translation="MIGKGAALPAGSASRTRPQQGAGGIEPAELAELRQAVLHLRRHF
                     ARPRADPAGTELSLTAHFLNLAPSTTVRFRLWMGLCTQAATELLGDPLAHLRPVDTLD
                     SVMVVQLLDRVADRLGLSSHVC"
     gene            complement(10071..10940)
                     /locus_tag="P5558_05005"
     CDS             complement(10071..10940)
                     /locus_tag="P5558_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRY23699.1"
                     /translation="MRRNLDTGLLRAFVAVAESGGMTAAARQLHLTQAAISQQIRRLE
                     DILERPLFERGRRGPTLTTAGGMLLARAEKMLALNDEIMATMTAPAFEGEVRIGVPQD
                     IVSPYLAPVLKSFDKAWPRVRVFIVAGVTLELLELLKQGEIDLCLTTEPAPQKGGETL
                     LLDPLAWVGAPGGNAHRRDPLPVALGDPSCVFRQAILAALARADRDWRSVCETSNMAP
                     LCVSVEADLGVTALLESSVPPHLALLGKDSHLPPLPDFHICLYMPQAGASEIAQEFAR
                     HIRQSLAPLRRAA"
     gene            11112..11513
                     /locus_tag="P5558_05010"
     CDS             11112..11513
                     /locus_tag="P5558_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HRY23700.1"
                     /translation="MSEALIQRYYAAFNRGDSEALLACLADDVAHEVNQGETRHGIEA
                     FRAFAAHMARCYQEELVDIVVMTEPSGERAAAEFIVRGRYIATDEGLPEARGQTYELR
                     AGTFFAIRDGRISRVTTCYNLQDWIAQVRGG"
     gene            11506..12105
                     /locus_tag="P5558_05015"
     CDS             11506..12105
                     /locus_tag="P5558_05015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY23701.1"
                     /translation="MAEPDPVRVVPLAGPAIAPLLPALAELRIQVFRDFPYLYDGDLA
                     YERWYLERFAAAEQALVVAALDGERLVGAATAAPLAAEHEDFRAPFIRLGIDPADVFY
                     LAESVLLGGFRGQGIGHAFFDEREAAGRRLGFGKAAFCAVVRPLDHPLRPPGYRPLDG
                     FWRRRGYVPVPGMTTTFPWKDLDEAEASPKPMQFWLREL"
     gene            12107..13429
                     /locus_tag="P5558_05020"
     CDS             12107..13429
                     /locus_tag="P5558_05020"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRY23702.1"
                     /translation="MRRIPLILGLLCLLLAGPLRAATLDEVIRAHGFAPEAVGIVVED
                     AATGRRLVEHRADRPGPAASTLKLVTAIGVLDALGPEHRFATRVLATGPVDRAGRLAG
                     DLVLAGGGDPLFDIDGLMALAWALRRAGIEAVAGRFILDDASLPRLPVVNPKQPVEAG
                     YNAGIGALSLAFNRVRRAAGGLYTVPNLRERGPAWGRLPMGQAASVPVQDAGLHAALV
                     FRDLASSLGIGLPEPVRAAVPAGAGLVAEILSRPLRDIVQAMLLYSNNQIAEILGLEA
                     TGAGSLARSAEMLAAHVEAALPDVDWRGFVLTNHSGLDPAARASPDQLLAILTLAEAR
                     HDIISLLPAAAWSGSLERRLDEPATALRVWAKTGSLDFASALLGYVLPQAGGPRRIAV
                     LIEDAAGRRARDAVEVPPPAMRRAIDDFTLRARDLRDALAVWALELPG"
     gene            complement(13700..14539)
                     /locus_tag="P5558_05025"
     CDS             complement(13700..14539)
                     /locus_tag="P5558_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY23703.1"
                     /translation="MLARSLATLLLLALPAVAAADTLGGIKERGTIRLGVRADAIPFS
                     FTDTLGERGGYTVQLCRAVSADLKKELGLAELKVEYVEVTTEDRFAALNDGRIDILCG
                     ATSVTLSRLDEVDFSSATFVTGMGVLYRNDGPTSFAELAGKKIGVRGGTTTEETLKRE
                     LDQAGLTVEIVSIDDHEAALEELAEGKIDAYFADRAILIYLLQASGKADVLTVGERLY
                     TIESYALALPLDDRDFRIAVDRALSRIYRSPMLDQIYTASFGDQQPGDILRALYVLGA
                     IPD"
     gene            complement(14589..16574)
                     /locus_tag="P5558_05030"
     CDS             complement(14589..16574)
                     /locus_tag="P5558_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HRY23704.1"
                     /translation="MFEKILIANRGEIACRIIRTCQRLGIGTVAVHSAADAMARHVAM
                     ADEKVAIGPAPAAESYLRIDRIVEACRQTGAQAVHPGYGFLSENAELPLALAAAGIVF
                     IGPPVEAIRAMGDKIESKRLARAAGVSTVPGTLDAIGDPTQAREIAAEIGYPVMVKAS
                     AGGGGKGMRIARSERELDEGLERARSEARSSFGDDRVFIEKFVEEPRHIEIQVLGDRH
                     GRTLHLGERECSIQRRHQKVIEEAPSPFLDAATRSSMGAQAVELARAVGYHSAGTVEF
                     IVDKDRHFYFLEMNTRLQVEHPVTELVTGIDLVEQMIRVAAGERLAFDQPDIRLDGWA
                     IEARVYAEDPARGFLPSTGRLVTYIEPSGPGVRCDSGVVEGGEVSLHYDPMIAKLCVH
                     APDRPAAIARLAEALDNFVIQGPGHNLSFLNAVVSHPRFAKGSFTTGFIAEEYGEKFT
                     APALDEATELRLVALASAMRARQLARAQQISGRLPGVNLAPPTSFVAYVSRTARTVEA
                     VERDGAFAVSIDGGPAHELVLHWRPGLPLARASIDGEMIAAGCRRLPEGFALTHRGSV
                     VQVLVRRRRAHELALLMPEKVPADTSKMLLSPMPGMIVMVDVVEGDLVKAGQTLCVLE
                     AMKMENVLKAERDGIIATVSIAERDTVSADQVLMTFE"
     gene            complement(16598..18130)
                     /locus_tag="P5558_05035"
     CDS             complement(16598..18130)
                     /locus_tag="P5558_05035"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRY23705.1"
                     /translation="MQEILDKLEAKREGARQGGGARRIEAQHGRGKLTARERLELLLD
                     AGSFEETDMFVEHRCTDFGMAEQRVPGDGVVTGQGTINGRLVFVFSQDFTVFGGSLSE
                     SHAEKICKVMDRALQVGAPVIGLNDSGGARIQEGVASLAGYADVFLRNVKASGVIPQI
                     SVICGPCAGGAVYSPAMTDFIFMVKDSSYLFVTGPEVVKTVTNEVVSQEELGGAGTHT
                     RRSGVADLAFENDVEAMAELRRFVDFLPASNLEKPPVWPTEDPALRDDFSLDTLVPAN
                     ANQAYDMKELVLKIVDEGDFFELQPDYAANIITGLARMEGSTIGIVANQPLVLAGCLD
                     IDSSRKAARFVRFCDCFNIPILTLVDVPGFLPGTAQEYGGIIKHGAKLLYAFAEATVP
                     KVTVITRKAYGGAYDVMSSKHLAGDVNYAWPTAEIAVMGPKGAVEIIFRSDLGDEGKI
                     AARTDEYRDRFANPFVAAGRGFIDDVIMPHGTRRRVAQSLRWLRSKKIQAPWRKHGNI
                     PL"
     gene            18343..19314
                     /locus_tag="P5558_05040"
     CDS             18343..19314
                     /locus_tag="P5558_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY23706.1"
                     /translation="MNRLLTGVAALGLVLALGGTAAAQDEKFITIGTGGQTGVYFVVG
                     QSICGLVNRGQAQHGIRCTAPSTGGSIANINAIREGSMDMGVAQSDWQFHAYNGTSQF
                     EEQGPFEDLRAVFSVHGEPFNVIARADSGIETFTDLKGKRVNVGNPGSGQLATMEVVM
                     EAMGWTMDDFALASQLQPAEQAAALGDNNVDAIIYTVGHPNGSIQEATTTVDARLIPV
                     DTPEIAKLVEERPYYAWATIPGGMYTGTDEDVKTFGVKATFVTSASVDDEVIYQVVKA
                     VFDNFDRFKSLHPAFANLTEEEMVTDGLSAPLHPGAERYYRERGWIQ"
     gene            19396..21984
                     /locus_tag="P5558_05045"
     CDS             19396..21984
                     /locus_tag="P5558_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015088441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRY23707.1"
                     /translation="MNERSGAAILEPRGKSAAELQDIVAAADTGARNPLGPTGKLLFL
                     TALAWSLFQLWIASPLPFMLRFGVFNSTEARSIHLAFAVFLAFTSYPAFASSPRDHVP
                     IADWIMAILGAASAAYLYVFYASISSRVGAPIAQDYVAAVIGMLLLLEATRRALGPAL
                     AIVAIVFLAYTFLGPYMPGIIAHKGNSLGEVVNHHWITTSGVFGIAVGVSTSFVFLFV
                     LFGALLDKGGAGNYFIQVAFSLMGHMRGGPAKAAVVSSAMTGLISGSSIANVVTTGTF
                     TIPLMKKVGFSSEKAGAVEVASSVNGQIMPPVMGAAAFLMVEYVGIPYFDVVKHAFLP
                     ATISYIALVYIVHLEALKAGMEGLPRAYEPPPLLRRLIGLVFGVAVVSAVSLAVYYGI
                     GWLRPAFGPAANWIVAIGIVVVYVALVAFGARYPELEPDDPNAPTLTLPLPGPTVKSG
                     LHFLLPVVVLVWALMVERLSPGLSAFYGTVFMIFILVTQRPLFAFFRRQGDLGGAARR
                     GFDDLVQGLVAGARNMIGIGIATAAAGIIVGVVSQTGVGSALAEVVAFLSGGSIIAIL
                     LLTAVLSLILGMGLPTTANYIVVSALLAPVIVTLGQQNGLLVPLIAVHLFVFYFGIMA
                     DVTPPVGLASFAAAAVSGGDPIKTGVVAFWYSMRTAALPFLFIFNPQLLLIDVGWVEG
                     ILVFVVAVIAMLTFAAALQGWFITRSRIWESALLLLIAFSFFRPGFWMDMISPPYEIR
                     EPAAFTEVVGLVPPGTLLQTRIAGLDQFGAPTQFATLLEVPEGATGEERVQAMGITLL
                     ERDDKQIIDNVAFDSAAQKAGLDWDQELLFFREPLPQPSKYWIFIPTLLVLAGLVALQ
                     RRRVKA"
     gene            22055..22480
                     /locus_tag="P5558_05050"
     CDS             22055..22480
                     /locus_tag="P5558_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY23708.1"
                     /translation="MFRDILVTVDPDSPESYARALPQAVELARTHGATLHVMTVVPDF
                     GSSMVASFFPAGFEKKALGRAEEALLELTGTHVPVELEHQIVVAHGRIYQEICRVAEE
                     VKADLVVMASHKPAARDKFVAPNAQQVLQRTSASVLIVR"
     gene            complement(22780..24360)
                     /locus_tag="P5558_05055"
     CDS             complement(22780..24360)
                     /locus_tag="P5558_05055"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRY23709.1"
                     /translation="MLVALDSLETGGDRPCDLAIVGGGAAGLGLALGLAGSGLEIVLA
                     EAGDEAFEPWHQSFCAGSSIGNLPLDPETTRLRQLGGSTNHWGGRSRPLDAEALAARP
                     WLGLPAWPLEAGELRRHLAAACRLLEIDRPPDEPWDAPADSDAAAQALGGPTLTPAVW
                     RFSPPTRMGERWRTELAARADLTLLLDADLVDIELAPSLDRIQWLDLRSRPGKTLRLR
                     PKVTVLAMGGIENARLLLACRRQLAAGIGNRHDQVGRHFMGHPIFEPLQLSLAEGARL
                     PELLHDRDTPAHGHIEGLLHLNRAGQEALEATAVDAAFYSDGQFGPPGFRAARRLQRK
                     LGHGQLDASLIRDGGEMLGDLPGLVHESLVKLGWLAPETGRLTVSTLIEQCPDPASRI
                     TLDQAVDAFGRPRARVDWRLGETDRRTVRRFARHLADHLAARGLGRAVLAPWVIDENL
                     PFPPHGNSAHHMGATRMSHSPRTGVVDPDCRVHGMTDLYIAGSSVFPTSGSQHPTLNL
                     VMLTLRLAAHLTRRLGHA"
     gene            complement(24438..>24806)
                     /locus_tag="P5558_05060"
     CDS             complement(24438..>24806)
                     /locus_tag="P5558_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY23710.1"
                     /translation="KDFLPYCAAEGIGVLLAGPFNSGILATGAKAGATFFYAPADTEI
                     VERTSRIEAICAAHNVPLAAAALQFSASHPAIASVVTGCRSATEVERNLALMALPIPP
                     ALWQELATAGLIARETPLTL"
BASE COUNT         3581 a         8423 c         8992 g         3810 t
ORIGIN      
        1 tgtcgcggct gatcacgtcc tgcgacggca cgtcgaggac gcgggtgcgc agatcgacgc
       61 ggaccatggt ctgcaggccc gggacgacga ggatcaggcc ggggcccttc accttccaga
      121 accggccgag caggaagacg acggcccgct cgtactcgcg catgatcttc agcgagatca
      181 ggatgatgaa gagcgggacg agcagcagcg gcagcaggag cacggggtcg tagtccatca
      241 gcgggctcag cctccaggtg gttggggggc gacgacgagc accaggccgt cgcgcgcctc
      301 gacggtgacc ggcgtgtcgc tggcgaggtc gtccgggccg cgggccgccc agatctcgcc
      361 ctcgagccgg acatggccct cgccatcggc ccagtcgacg accgtgcccc ggccggcgag
      421 cacggcctcg gccccggtca cgaccggtcg ccgatggctg cgcagcgcca ggccgagcgc
      481 caggaggaag atgccgctgg tgacgatcgc caccgcaccg atgagccagg gcgagacgcc
      541 gaagccggga acatccgtgt cgaccaagag cacggcaccg gcgacgaagg cgacgatgcc
      601 gccgaggccc agcgcaccga agctcggcac gaaggcctcg gcgatgacca ggatgatgcc
      661 gaggagcagc aaggcgaggc cggcgaagga gacgggcagg acctggagcg cgtagaggcc
      721 gagcagcagg cagatggcgc cgacgaggcc ggggccgacg aggcccggcg aatagagctc
      781 gaagatgatg ccgtagatgc cgacgagcat caggatggcg gcgacgttcg ggtcggtgat
      841 gatggcgagg agctggtagc gccagtccgg ctggctctcg acgatggcgg ggtcggcggc
      901 gtcgatgagg gccgggccgc ttttcagctc gaccatgcgg ccgtcgaccc gccggagcag
      961 ctcggcccgg tccggggcga tgagctcgat gacgccggcc ttcagcgcct cctcggcggt
     1021 caggcttttt gcctcgcgca cagcggactc ggcgaatttc gggtcgcggt cgcgcatggt
     1081 ggcgagcgag cggagcaggg cggccgcgtc ctccatcgcc ttggtggcat gcgcggacct
     1141 ttcgcccgca tcgccgccga tctggacggg tgtcgccgcg ccgagattgg tgccgggcgc
     1201 catggcggcg agatgggtgg cgtagaggat gaaggtgccg gcgctcgccg cccgcgcccc
     1261 cggcggggcg acatagccga tgacgggcaa gggtgcgtcc aggatcgcct cgaccatggt
     1321 gcgcatcgag gtgtcgaggc cgcccggcgt gtcgatctcg agcagcacga gatcggcgcc
     1381 gtcggcggcc gcggcctcga tcgcccggac gaggaaatcg ctggtggcgg ggccgatggg
     1441 gcccttgacg ggcacgacct gcacggtggt ggcttcggcc gatgccggca tgccgagcag
     1501 gggcagcagc atgccaagca ggaaaaggag ggacgcgagg gccgaactgc gcttcattgt
     1561 ccgaccatcg acgagggcac cgatgccgtc aagagatgca cgattgcgag agatgcggca
     1621 ggtttctcca tccgggggtt gatacatgac cacgctgacc ctgacgagct ggaacatcga
     1681 gcatttctcg cggctgctcg acggcaatgc cggcgaggcg gagcggctcg ccgccatcgc
     1741 cgccgagatc cgggccatcg atccgcacgt cctcctgatc gtcgaggggc cggccagcct
     1801 gccggcgctc gaggcctggg tggcggcggc cgagccgcac ggcctcggcg gcggctggcg
     1861 cgtggccacc attcccggca cgaacgaggc gctcgaggca cggccggacg acccgcggcg
     1921 ggcgctggcc gggctctacg ccatgcaggg caaccggatc agcggcggcc agtggctctg
     1981 gttcctggtc aaggacgggc tcttccagga gagccgggcc acgctcctcg caccgctggt
     2041 ctggcaggac ctgacggggc agcgggactg gccggtgcat cgctggggcg agctcatatc
     2101 gcggcggcac gcgcactggc ggcacccgca ggtgctcctg ctgcagatgg gcggggtgga
     2161 gatcgaggtc gtcggagcgc atctgaagag caagatcaac cacgagcgcg ccttcgacga
     2221 ggcgggcgag ctgacccacg actatgtcga cgaggccctt cgggcgcgca tccagctcgc
     2281 caccgaggcc tacgacatcc gccgctacat cgacggccgc ttcgcccaga cgccgaagcc
     2341 ccgcatcctg gtggcgggcg atttcaacga cgggccgggg cgcggcttct tcgagcgcga
     2401 gtatctctat ttcgacctcg tcggaaacct gcagggcgac gtcttcgctg ccgacaagtt
     2461 cctcaaccac gcgctcttcg acttcccggg cgacctgcgc tggaccaccg ccttcgacga
     2521 ccgggtcgag cgctggtcga ggacacggga cggcacgccg cccttgccgg ccgagcccta
     2581 cgatgcgacc cgcttccagc tcatcgacca catcctcttc acccaggcgc tggtgggcga
     2641 gggggcatgc ccgcggatca ggggcggggc aggccgggtc gagcataccg tgcaccagcg
     2701 gatcaatgcc gggctcggcc gccggcgcac cagcgaccac gtgccggtct cggtcgagct
     2761 cacgctctga agaacgcctc cagcttctcg gtcgtcgcct cgggcgcctc ctcggggagg
     2821 tagtggccgc aatcgagcgc ctcgccggtc acctcgcccg ccgccttctc gcgccaggtg
     2881 gcgagcacgt cgaaatgctt ctccatgagc ccgtggcggc cccagagcac gaggaggggg
     2941 cactggatgc gggcgtcctc gtcggcctcg tcgtgctcga ggtcgatcgt ggcggcggcg
     3001 cggtagtcct cgcagctggc atggatggtg gccgggctgg cgaagcagcg ctcgtactcg
     3061 gccatggcgt ccgggtggac gaacgtgctg ctcttgccgg ccgaccagcc gccgagcttc
     3121 tggtgcaggt agtagatcgg gtcggcgccg atcagccgct cggggaaatc ggccgggcgg
     3181 ctgaggaaga accagtggta gtagccctcg gcgatcgcct tgttggtggc ggcgaagatg
     3241 gtgcgggtcg gcacgatgtc gagcacggcc gcccgggtca ccaccttggg gtggtcgagg
     3301 cacatgcggt gcgtgacccg gccaccccgg tcgtggccgg ccaccatgaa ccggtcgaag
     3361 ccgagcatgc gcatggcctc gaccaggtcg gccgccatcg cccgcttgct catcgcccgg
     3421 tgggtcccgt cgccgaccgg cttgccgctg tcgccatagc cgcgcagatc ggcgcagacg
     3481 acggtgaagg tctcggccag ggcgggggcc acgcggtgcc acatggcatg ggtctgggga
     3541 tagccgtgca gcagcaggag cggcggccct tcgcccttgg tgcgggcgaa gatcccggtc
     3601 tccgccgtgc tcagccgatg cccctgaaag ccaggaaaga gtttcattcc accgccctcc
     3661 gactcgcgcg cctgggtatt tctagcacac gcgaaggtcg atggcgcggc ccaagccgtc
     3721 gaccgtgcgg gcggggcgca gctcccagtg gagccggggc gtgaagccct ggccaagggc
     3781 cgaatcgaag gttcgggcca tgacgatccc ggcggaccgt tcgaccttgg tgtgcttctc
     3841 cagcttggcg gcgaggttaa cgggctcgcc caggaccgtg tattcgagcc tttgcgcgtc
     3901 gcccaccgtg ccgaacatca cggtgcccgt cgtgatcgcg gcggcgaccg cgggggccgg
     3961 caggttgcgc cgggcgcgat cgccgctcca gcgttggccg gtctccagca cggcctcgat
     4021 cgcggcgagc gcctcggcgg cggcggaatc gctcggccgg acggcaccga agctggcgag
     4081 gatgccgtcg cccatgaact tgtcgatgct gccgccggcg gcgcgcaccg cccgggtcat
     4141 cagggcctgg tactcgccga ggagctgcat gacgtccgcc ggcgccaggc gctcgccgag
     4201 gcctgtgaag ccgcgcagat cgatgaacat gatcgcggcc tcgcgcagct cggcgcggcc
     4261 gggcacgagc tcagcgtcgc tgccggtgat ccggccggcg atctcgggcg cgaagaagcg
     4321 cttgaggtcg gacgcggcct ggccctcgac ggcggcccgc accaggagct tgcgggcccg
     4381 gtggagggcc agggcgagga tggcggtcac cacgaggatc gagacgatct tgtcgacctc
     4441 ggcgccgatc aggatgctgt agctcatcat gtattcggcg aagtcccggg tgatcatcgt
     4501 ttcgcctgcc ggcccgccgc cggcgagcac cgcgtaggcg acgagggcga gccagccgag
     4561 tgctgcgctg atgccggcga ggaggacgaa ggcgggctcg aagcggagcg tgcgaagggc
     4621 gatcaggatg aagacgtaca tcaaggtcgg cgccttgagg tagagggccg gcggcgcctg
     4681 gtactgcagg tggaagctcc agatggtcac catcaggagg gcgatgtcga cgaccaccga
     4741 gacggcgagg atcggccggg tcaggcgctg cctcagggcg aggccgaggc gccagaaggt
     4801 gaacagggca tagagcacga gggcgatcgg cacgggctcg aacggcacgt ccggcgggaa
     4861 ggccttgggg gtgaggctgt agaggacggc gaagaggacg atggcgccga gctggatcag
     4921 gctgaccagc acctcgctgc gcgcctgctc gcggccgatg gcgtcggcca cgcgctgcgg
     4981 catggcttcg ggcaagggcg ggccgagcag gacacgcccg gcacgctcgc gggcagcgcc
     5041 gctggccagg cgctggacga ttccgcgcat gggcggcctc cggaattgcg ccaacgaggt
     5101 ttacgccgaa cgacgatccg caccaaccgg ccgggtgctc gcggcgcatc acgtcacgct
     5161 gagccgggca gcatggacgc cggtgaatga aggaaacgtg acaccggctc atcccggccg
     5221 gcaatgctgc attgcaaaaa ccggcatggt gaccggccat gagagtcgcc acattcccga
     5281 tcgttgggag caacgcgtgg atgatgcgtt gcaatatgag ttcgaaggac ctgtaccatg
     5341 aacaaccaga tgaccctgcc tgtcggtggc gtcgcgggtg cctggcgcgg cgagagccgc
     5401 gacacctcgc gcttcggtca gctgattgcc gccctgcgcc gccagctggc cgttcaccgc
     5461 gtctatcggc ggacctacga tgagttgatg acgctgaccg atcgggagct cgacgacatc
     5521 ggtgtcgccc gctgcgatat ccagagggtc gcccgcgata cggcacggga ggtcgtgccc
     5581 gcgtaagcgc cccgggccgg cgccgatgac gcctttgggt gtcgtcggcg gccgtgtcct
     5641 gatacgcagg ccttctcacg actcggggag gtcggcgcat gaatctcgag cagttccgtc
     5701 aggaggcgca cggcctcgtc gactggatgg tggcatatct ttcgaccatc gagcggcggc
     5761 ccgtgcgcag cccggcgacc ccgggcagca tcgccgcgca gctgccggcc gcggcaccgg
     5821 aaggccccga gccgttcgag gccatcttcc gcgacttcga gcggatcgtc atgcccggca
     5881 tgacgcactg gcagcatccg cgcttcttcg cctatttccc cgccaattcc agcccgccct
     5941 cggtcctggc ggagatgctg acggcgacgc tcgccgccca gtgcatgctc tggcagacct
     6001 cgccggcggc gtcggagatg gagggccggg tgctcgactg gctccggcag atgctggggc
     6061 tggatgcggg cctcgccggg gtgatccagg actcggcgtc ctcggcgacg ctctgcgccg
     6121 ttctcgtggc acgcgagcgg gcgaccggct ggcaggccaa tgaggccggg ctgggcgctt
     6181 gtcccccgct tgccgtctac acgtccgatc aggcgcattc ctcggtcgag aaggccgtcc
     6241 gcatcgccgg tctcggccgc tcggccttgc ggctggtgcc gtcggacgag cgcttcgcca
     6301 tgcgtgccga tgccctggcc gggatgatcg aggccgatct gcgggccggg gtgaagcccg
     6361 cctgcgtggt ggccacgctc ggggccaccg gcaccggcgg catcgacccg ctggccgcca
     6421 tcggccggct ctgccgcgcg cacgacatct atctgcatgt cgacgcggcc tgggcgggca
     6481 gcgccctgct cctgcccgag acgcgctgga tgatcgacgg catcgagcag gcggacagct
     6541 tcgtcaccaa cccgcacaag tggctccgca ccaatttcga ctgctcggtg cattacgtgc
     6601 gccggccgga cgagctggtc cgcacgctct cggtgatgcc ggcctatctc accggcccgg
     6661 ggacggggca ggcggtcgac taccgcgact ggggcatccc gcttggccgg cggtttcgcg
     6721 cgctgaagct gtggttcgtg atccgctcct atggcatctc cggcctgcag gcgatgctgc
     6781 gcgaccatat cgcctggacc cgcgagctgg ccgcctggat cgaggcgaca cccggcttcg
     6841 agctggtctc gccgccggtg ctcggcctcc tctcgttccg ctgccggccc tccgggctgg
     6901 acgccgaggg gctcgatgcg ctgaaccggc ggctggtcga gcggatcaac gacgacgggc
     6961 gcatctatct gacccagaac atcgtgcgcg gtcgcttcgc gctccgtttc tccatcggcc
     7021 aggcgacgac cgagcgacgg catgtcgagg aaggctgggc agtcgtccgg gagctggcgg
     7081 agcaagtagt acgaacaaga aatgtataaa aagagatagc gggctcggag cggttccccg
     7141 gctcttccgt cgcgtcggca tcgagcactg caaggtggcg gtccatatcc ctgctcagta
     7201 acggcgaatg gacgatcgac gaggggcgct tcgtgacgcc accacgatgc cggcacgatg
     7261 ccggcacgat cgacctggca ggcgcggcgc gcttcaggcg gctttcagcc cggcgctctg
     7321 caggccgccg agcaggtcct cgacgagatc cggcggtagc tgccagtagc ccaggtactc
     7381 gccggcccgg tcggcggcgt ccggggccag ctccaggagg tcctggacgg cctgcgccgc
     7441 ctgccgctgc aggccgagcc ggccatagac ggcggcgcgc agcaggcggg tcatcaggaa
     7501 atccggcagg tcgctctgct cgagctcgcc tctggccgcc gcatagtccc cgcgccgata
     7561 ggcgtcgatg acgaagggga tgcggtagga ggccgggtgc gccggattca gctcgatcgc
     7621 ccgggcgcgc atggcgaggc cgcgctccca gtcgcccgaa taggcgatct tgccgccgag
     7681 atcggcgagc gcgtcgctgt cgttcgggtt gagctcgagg gccagatcgg cgtgccggcg
     7741 aaacccgtcc atgtcgccgg cggtgaacag caccgctgcc agcgaccgct gggcgaccgc
     7801 attctccggc tccagctcga ccgccttgcg cgccgcctcg agcgccgcgg ccagctcctg
     7861 acccgccggg tcgtgggtgt agccgtagcg cagcgcctcg aggcgaagat aggcgagata
     7921 ggcccaggca tgggcatagt ccggctccgt gcggaccgcg cgctcgaggc aggttttcgc
     7981 tgccgtgtag tccgccggcg cataggtcgc atagtagtga tcggcggcca ggatgcaggc
     8041 atagctcgcg aggctcgccg gcaggtccgc ctggtcggcg gcgcggctga gctggcggtt
     8101 cacgaggccc cagggctggg cgacggcggt gacgatggcg gcagcgatct cgctttccag
     8161 cgccagggcc ccgttgccgg cgtgcgggcc ggtgaaggtc ctggtccaga gcacaccggc
     8221 gtcctcggtc gagatcaggc tggcggtgac gacgaacccc tcgtcgtcgc gccgcaccgt
     8281 gccatcgacg acgtagtcga ggcccgcttc ggcggcagcg cggatcgggt cgccgccggc
     8341 ctcggccagg gccagggcgc tgcgtcgggc caatacgaag aactggcgga aatgcgtcag
     8401 ttcggcggtg acgagggcgg tgaggccacc ggccacggtg gtggtgcccg gcgcgtcgtc
     8461 gagcgcttcg aacggcagga cgccgatggc cggcgtctgg cgtgcgaggt cggcagcacc
     8521 attgacctcg accggcgctg tcggtgagcc acgctcgagg ccgagatggg cggcgatgcc
     8581 gatcgcgatc gcgatgaggg cggcggtcag gaaggcgacg cgatacggcc ggcgatggct
     8641 gggcgcgggc ttcgcccggg tgagggcggg agcccgggcg gcggtccggg ccggggccgg
     8701 tgctccggtc cgcgctgcct cgaagcgggg cacgtagccg cccttgggca gggtgatgcg
     8761 gaccgggtca gccttgcctt ccccggcata gtaggcggcg agcttctgcc tgagacggcc
     8821 ggcctcgacg cggaccagcg actcggcctg cgggtcgaaa tccgccggcc gctcgaacac
     8881 gtcgacgccg atggcgtagg cgcgcatggc ccgctcgttg ccggcgagcc gggcttcgac
     8941 gatgtggcgc aggaaggcct gcaggcgcgg cgagccctcg agctcggcgc tggcgagcac
     9001 acgcgcgagg gcggcgagca cagcttcgtc cgggatcggt tcctcgccca tcctcctcca
     9061 ccggcggcca gcgccggcac catccggcca taagttgcag cccgatcatg ggccgcgact
     9121 gaccgtcagt tgccgttact cacatgccgc gcaggcccaa ggtatgctcc gactgcggct
     9181 cctcgaacag tgtgatcgaa ggggcggcaa tggcaagtga ggcgaatccc ctggggtcgg
     9241 gaggcccttc tctcggcctc gagctctggc ggggacgcac cggcgtgtcg agcctccctc
     9301 ggtccgaaat tctcgagatc cggcgtgcca accggcgagt gagaggcttc ctcgccgggc
     9361 acgccggtcg ttcgcgtggc gccgaagcac gcgatggcga gggtcggccg gtggacccga
     9421 tggacccgcg cgcccgaagc ttcagcctcg tcggcgcctt tctcgccgcc gctcggccct
     9481 cgccctggct ctttcgtcgg tggatgaccg ccgcccacga ggccgccgcc gagatcctgg
     9541 gtgatccgga cgccgatgtc ttcgcgtaca atgacgcggc cgagatcgat caggaagggg
     9601 tgatggccct gctcgacgag atagatgccc gggtgacacc gctggtggac gtggacctgc
     9661 cgacgccgcc cgggtcgggc cgatgatcgg caagggagcg gccctgccgg ccgggtcggc
     9721 ctcgcggacc aggccgcaac agggggcggg cggcatcgag cccgccgagc ttgccgagct
     9781 tcgccaggcg gtcctgcatc tgcggcggca tttcgcccgg ccgcgcgccg atccggccgg
     9841 gaccgagctt tccctgaccg ctcatttcct caatctggcg ccctcgacga ccgtccgctt
     9901 ccggctgtgg atgggcctgt gcacccaggc ggcgaccgag cttctgggcg acccgctggc
     9961 ccatctgcgg ccggtcgaca cgctcgacag cgtcatggtg gtgcagttgc tggaccgtgt
    10021 cgccgaccgg ctcgggctct cctcgcacgt ctgctgagcg gctcgggctc tcaggcggcc
    10081 cggcgcagcg gggcgagcga ctgccggatg tggcgggcga attcctgggc gatctcgctg
    10141 gccccggcct gcggcatgta gaggcagatg tggaagtccg gcagcggcgg caggtggctg
    10201 tccttgccga ggagtgccag atgcggcggc accgacgact cgagcagggc ggtgacgccg
    10261 agatcggcct ccacgctgac gcagagcggc gccatgttgc tggtctcgca gaccgaccgc
    10321 cagtcgcggt cggcccgggc gagggctgcc aggatggcct ggcggaagac gcagctcggg
    10381 tcgccgaggg ccaccggcag ggggtcgcgc cgatgggcgt tgccgccggg tgcgccgacc
    10441 caggcgagcg gatccaggag cagcgtctcg ccacccttct gcggcgccgg ctcggtcgtc
    10501 aggcagaggt cgatctcgcc ctgcttcaag agctcgagca gctccagcgt cacgcccgcc
    10561 acgatgaaga cccggaccct gggccaggcc ttgtcgaagc tcttcaggac gggtgccagg
    10621 tagggcgaga cgatgtcttg cggcacgccg atgcgcacct cgccctcgaa ggccggtgcc
    10681 gtcatggtcg ccatgatctc gtcgttcagc gccagcatct tctccgcccg ggcgagcagc
    10741 atgcccccgg cggtggtgag cgtcgggccg cgccggccgc gctcgaagag cggccgctcc
    10801 aggatgtcct cgaggcggcg gatctgctgg ctgatcgctg cctgggtgag atggagctgg
    10861 cgggcagccg cggtcatgcc gccgctctcg gcgacggcga cgaaggcgcg cagcagcccg
    10921 gtgtcgagat tgcgccgcat gctttccccc gctgggtttg ccgataacga accgttattc
    10981 aatgggctat cagcatcagc atttcttatg ctgcggccgc gcccggcctg tcaagccggc
    11041 ccgtcgcttg ccccggccgg acccgtcggt tagcaagccc gggtgatcgc agcgaagggt
    11101 gccgaaggcc gatgagcgaa gccctgatcc agcgctacta cgccgccttc aaccgcggcg
    11161 acagcgaggc gctgctggcc tgcctcgccg atgacgtggc gcacgaggtg aaccagggcg
    11221 aaacgcgcca cggcatcgag gcctttcgcg cgttcgcggc tcacatggcc cgctgctacc
    11281 aggaggagct ggtcgacatc gtcgtcatga ccgagccatc gggcgagcgg gcggcggcgg
    11341 agttcattgt gcgcggccgc tacatcgcca ccgacgaggg gctgcccgag gcgcggggcc
    11401 agacctacga gctgcgggcc ggcacgttct tcgccataag ggacggccgg atcagccggg
    11461 tgacgacctg ctacaacctg caggactgga tcgcccaggt gcggggtggc tgagcccgac
    11521 ccggtccggg tggtgccgct ggccgggccg gcgatcgccc cgctgctgcc ggcgctggcc
    11581 gagcttcgca tccaggtctt tcgcgacttt ccctatctct atgacggcga tctcgcctac
    11641 gagcgctggt atctggaacg cttcgccgcg gccgagcagg cgctggtggt ggccgccctt
    11701 gatggcgagc ggctcgtcgg ggccgccacg gcggcaccgc tcgccgccga gcacgaggat
    11761 ttcagagcac cgttcatccg cctcggcatc gaccccgcag acgtcttcta tctcgccgaa
    11821 tcggtgctgc tcgggggctt tcggggccaa ggcatcggcc atgccttctt cgacgagcgg
    11881 gaggccgccg ggcggcggct cggcttcggg aaggcggcct tctgcgccgt ggtgcggccc
    11941 ctggatcacc cgctccgccc gcccggctac cggccgctcg acggtttctg gcgtcgccgt
    12001 ggctatgtgc cggtgcccgg catgaccacg accttcccct ggaaggacct ggacgaggcg
    12061 gaagccagcc cgaagccgat gcagttctgg ctgcgggagc tctgagatgc ggcgaatccc
    12121 gctgatcctc ggtctcctct gcctgctgct cgccggtccg ctgcgggcgg cgacgctgga
    12181 cgaggtgatc cgggcgcacg gcttcgcccc ggaggccgtc ggcatcgtcg tcgaggatgc
    12241 ggccacgggc cgtcgcctcg tcgagcatcg ggcagaccgg ccgggccccg cggcctcgac
    12301 gctgaagctg gtgaccgcca tcggcgtgct cgatgccctg gggcccgagc accgcttcgc
    12361 cacgcgcgtg ctggcgaccg ggcccgtgga ccgcgccggc cggctcgccg gcgatctcgt
    12421 gctcgcgggc ggcggcgatc cgctcttcga catcgacggg ctcatggcgc tcgcctgggc
    12481 gctgcggcgg gccgggatcg aggcggtcgc gggccgcttc atcctggacg acgccagcct
    12541 gccgcgcctg cccgtcgtca acccgaagca gccggtcgag gccggctaca atgcggggat
    12601 cggtgccctc tcgctcgcct tcaaccgggt gcgccgggcg gccggcgggc tctataccgt
    12661 gccgaacctg cgcgagcgcg ggccggcctg ggggcggctg cccatgggcc aggcggccag
    12721 cgtgccggtg caggacgcgg gcctgcatgc cgccctcgtc tttcgcgacc tcgcttcctc
    12781 gctcgggatc ggcctgccgg agccggtccg ggccgccgtg ccggccgggg ccgggctcgt
    12841 cgccgagata ctgagccggc ccttgcgcga catcgtccag gcgatgctgc tctacagcaa
    12901 caaccagatc gccgagatcc tcgggctcga ggcgaccggg gcgggctcgc tcgcccgttc
    12961 ggccgagatg ctcgcggcgc atgtcgaggc ggcgctgccg gacgtcgact ggcggggctt
    13021 cgtgctcacc aaccattccg ggctcgaccc ggcggcgcgg gcgtcaccgg accagcttct
    13081 cgcgatcctg acgctcgccg aggcgcggca cgacatcatc tcgctgctgc cggccgccgc
    13141 ctggtcgggc agcctggagc gccgcctcga cgagccggcg acggcgctca gggtctgggc
    13201 caagaccggc tcgctcgatt tcgccagtgc gctcctgggc tacgtgctgc cgcaggcggg
    13261 cgggccgcgc cgcatcgccg tgctgatcga ggacgcggcg ggccgccggg cgcgcgacgc
    13321 cgtcgaggtg ccgccgccgg cgatgcgccg cgccatcgac gacttcacgc tccgcgcccg
    13381 tgacctgcgc gatgccctgg cggtctgggc gctggagctg ccggggtagg agcctgtccg
    13441 agcaatggtc atgacctgca tcgtcccagg gcaagcgagg gccaggaacg gcggcgaagg
    13501 cgatgccggg gcatcgtcga gccggcgtga cgccgccctc gcttgccctg ggacgacccg
    13561 aagggcgcgc cattcacggc tgggggccat gtcgaccaac cctttcgatg gccccgcatc
    13621 gctgcgggcc agtctcctcg cccgcagccg gtaatggcgc gtgcaggcca tgaccattgc
    13681 tcggacaggc tcctagggtt cagtcgggga tggcaccgag cacgtagagg gcgcgcagga
    13741 tgtcgcccgg ctgctggtcg ccgaagctgg ccgtgtagat ctggtcgagc atgggcgagc
    13801 ggtagatgcg gctgagcgcc cgatccaccg cgatcctgaa gtcgcggtcg tcgagcggca
    13861 gggccagcgc atagctctcg atggtgtaga gccgctcgcc gacggtcagc acgtcggcct
    13921 tgccgctcgc ctgcagcagg tagatgagga tggcacggtc ggcgaaatag gcgtcgatct
    13981 tgccttcggc gagctcctcg agggcggcct cgtggtcgtc gatcgacacg atctcgacgg
    14041 tcagcccggc ctggtcgagc tcgcgcttca gggtctcctc ggtggtcgtg ccgccgcgca
    14101 cgccgatctt cttgccggcg agctcggcga acgatgtcgg cccgtcattg cggtagagca
    14161 cacccatgcc ggtgacgaag gtggccgacg agaaatcgac ctcgtcgagc cgcgaaaggg
    14221 tcaccgacgt ggcgccgcag aggatgtcga tgcggccgtc gttcagggcg gcgaagcgat
    14281 cctcggtggt gacctcgaca tactcgacct tgagctcggc caggcccagc tccttcttca
    14341 ggtcggccga gaccgcccgg cagagctgaa ccgtgtagcc accgcgctcg cccagggtgt
    14401 cggtgaagga gaaggggatg gcgtcggcgc gcacgccgag gcggatcgtg ccgcgctcct
    14461 tgatcccgcc cagcgtgtcg gcggcggcaa cggccggcag ggcgagcagc aggagggtcg
    14521 cgagggaacg ggcgagcatg ggcagctcca gtcatgtggg cgtttcgggc ggccttgtgg
    14581 ccgcccattc attcgaacgt catgagcacc tgatcagcgc tcaccgtgtc gcgctcggcg
    14641 attgataccg tggcaataat gccgtcgcgc tcggccttca agacattctc catcttcatg
    14701 gcctcgagca cgcagagcgt ctgcccggcc ttgacgaggt cgccctcgac gacgtcgacc
    14761 atgacgatca tgccgggcat cggcgagagc agcatcttcg aggtgtcggc cggcaccttc
    14821 tcgggcatca gcagcgccag ctcgtgggcg cggcgacggc gcaccaggac ctggactacg
    14881 gatccccgat gcgtcagggc gaagccctcg ggcaggcggc ggcagccggc ggcgatcatc
    14941 tcgccgtcga tgctcgcacg ggcgagcggc aggcccggcc gccagtgcag gaccagctcg
    15001 tgggccggcc cgccatcgat ggagaccgca aaggcgccgt cccgctcgac ggcctcgacc
    15061 gtgcgggcgg tccggctgac ataggcgacg aacgaggtgg gtggagcgag gttgacgccc
    15121 ggcaggcggc cgctgatctg ctgcgcgcgg gcgagctggc gggcgcgcat ggcggaggcc
    15181 agcgccacca gccgcaattc ggtcgcctcg tcgagggcag gcgccgtgaa cttctcgccg
    15241 tattcctcgg cgatgaagcc ggtggtgaac gagcctttgg cgaagcgggg gtggctgacg
    15301 acggcgttga ggaacgagag gttgtggccc ggcccctgga tgacgaagtt gtcgagcgcc
    15361 tcggcgagcc gggcgatggc agccggccgg tccggcgcat ggacgcagag cttggcgatc
    15421 atggggtcgt agtgcagcga cacctcgccg ccctcgacga cgccgctgtc gcagcgcacg
    15481 cccggcccgc tgggctcgat ataggtcacg agccggccgg tcgaggggag gaagccgcgc
    15541 gccgggtcct cggcatagac cctggcctcg atcgcccagc cgtccaggcg aatgtccggc
    15601 tggtcgaagg ccaggcgctc gccggcggcg acgcggatca tctgctcgac gaggtcgatg
    15661 ccggtcacga gctcggtcac cggatgctcg acctgcaggc gcgtgttcat ctcgaggaag
    15721 tagaagtggc ggtccttgtc gacgatgaat tcgaccgtgc cggccgagtg gtagccgacg
    15781 gcccgcgcca gctcgacggc ctgggcaccc atgctgctgc gggtcgcagc atcgaggaac
    15841 ggggagggcg cctcctcgat caccttctgg tggcgcctct ggatggagca ctcgcgctcg
    15901 ccgagatgga gcgtgcggcc gtgccggtcg cccagcacct ggatctcgat atgccggggc
    15961 tcctcgacga acttctcgat gaagacccgg tcgtcgccga aggaggagcg cgcctcggag
    16021 cgcgcccgct cgaggccctc gtcgagctcc cgctcgctcc gggcgatgcg catgcccttg
    16081 ccgccgccgc cggccgaggc cttgaccatg acgggatagc cgatctcggc ggcgatctcg
    16141 cgtgcctggg tcgggtcgcc gatggcgtcg agcgtgccgg gcacggtcga gacgccggcg
    16201 gcgcgggcga gacgcttgct ctcgatcttg tcgcccatcg cgcggatggc ctcgaccggc
    16261 gggccgatga agacgatgcc cgcggccgcc agcgccaggg ggagctcggc gttctcggag
    16321 aggaagccgt agcccggatg cacggcctgg gcccccgtct ggcggcaagc ctcgacgatg
    16381 cggtcgatgc ggaggtagct ctccgctgct ggggcggggc cgatcgccac cttctcgtcg
    16441 gccatcgcca catggcgggc catggcatcc gccgccgagt ggacggccac tgtcccgatg
    16501 ccgagccttt ggcaggtgcg gatgatgcgg caggcgatct cgccacgatt ggcgatcagg
    16561 atcttctcga acacgggccc gttgctcctg atcggggtca gagggggatg ttgccgtgct
    16621 tgcgccacgg cgcctggatc ttcttggagc gcagccagcg cagcgactgc gccacccggc
    16681 ggcgggtgcc gtgcggcatg atcacgtcgt cgatgaagcc gcgcccggcc gcgacgaacg
    16741 ggttggcgaa gcggtcgcgg tactcgtccg tgcgcgcggc gatcttgccc tcgtcgccga
    16801 ggtcgctgcg gaagatgatc tcgaccgcac ccttcgggcc catgacggcg atctcggcgg
    16861 tgggccaggc gtagttgacg tcgccggcga ggtgcttcga gctcatcacg tcgtaggcgc
    16921 cgccataggc cttgcgggtg atcaccgtga ccttggggac cgtcgcctcg gcgaaggcgt
    16981 agagcagctt ggccccgtgc ttgatgatgc cgccatattc ctgggcggtg cccggcagga
    17041 agcccggcac gtcgacgagg gtcaggatcg ggatgttgaa gcagtcgcag aagcggacga
    17101 agcgcgccgc cttcctggag ctgtcgatgt cgaggcagcc ggcgagcacc agcggctggt
    17161 tggcgacgat gccgatggtc gagccttcca tccgggcgag gccggtgatg atgttggcgg
    17221 catagtccgg ctgcagctcg aagaagtcgc cctcgtcgac gatcttcagg accagctcct
    17281 tcatgtcgta ggcctggttc gcgttcgccg gcaccagcgt gtcgagcgag aagtcgtcgc
    17341 gcagcgccgg gtcctcggtc ggccagaccg gtggcttctc caggttcgac gccggcagga
    17401 aatcgacgaa gcggcgcagc tcggccatgg cctcgacgtc gttctcgaag gcgaggtcgg
    17461 cgacaccgga gcgcctggtg tgggtcccgg cgccgcccag ctcctcctgg ctgaccacct
    17521 cgttggtgac cgtcttcacc acctcggggc cggtgacgaa gagataggag ctgtccttga
    17581 ccatgaagat gaagtcggtc atcgccggcg agtagacggc accgccggcg caggggccgc
    17641 agatcacgct gatctgcggg atgacgcccg aggccttgac gttgcgcagg aagacgtcgg
    17701 cgtagccggc gagcgaggcg accccttcct ggatgcgcgc cccgccggaa tcgttgaggc
    17761 cgatgaccgg tgctcccacc tgcagcgccc ggtccatgac cttgcagatc ttctcggcgt
    17821 ggctttcgga gagcgagccg ccgaagacgg tgaagtcctg gctgaagacg aagacgaggc
    17881 ggccgttgat cgtgccctgg ccggtcacca cgccgtcgcc cggcacccgc tgctcggcca
    17941 tgccgaaatc ggtgcagcgg tgctcgacga acatgtccgt ctcctcgaac gagccggcgt
    18001 cgaggaggag ctccagccgc tcgcgggcgg tcagcttgcc gcggccgtgc tgcgcctcga
    18061 tccgacgcgc gccgccgccc tgccgggcgc cctcacgctt cgcttcgagc ttgtcgagga
    18121 tttcctgcac cggcccgctc cactccgtgt ttactggccc gcttccaccg tcgctgcccg
    18181 gcgcttatca tccggcgcga cggcacgcca gcacggcgcg gcgaaacgta acaagcgttc
    18241 acgatgtgac aacggcgttt tgacagcgtg acccgaggaa ctattgtacg gcgatcaagc
    18301 tctcgggggc acgatgctcg agagctcatg aggagggacg agatgaacag gctgctgacg
    18361 ggggtcgccg ccctcggtct ggttctggcg ctcggcggca ctgccgctgc gcaggacgaa
    18421 aaattcatca cgatcggcac gggtggccag accggcgtgt atttcgtcgt cggacagtcg
    18481 atctgcgggc tggtcaatcg tggccaggcg cagcacggca ttcgttgcac ggcgccgtcg
    18541 accggcggct cgatcgccaa catcaacgcg atccgcgaag gctccatgga catgggcgtc
    18601 gcgcagagcg actggcagtt ccatgcctat aacggcacgt cgcaattcga ggagcagggc
    18661 ccgttcgagg atctccgggc cgtcttctcg gttcatggcg agccgttcaa cgtgatcgcc
    18721 cgggccgatt ccggcatcga gaccttcacc gacctgaagg gcaagcgggt caatgtcggc
    18781 aatcccggct ccggccagct cgccacgatg gaggtggtga tggaggccat gggctggacc
    18841 atggacgatt ttgccctggc ctcgcagctc cagccggccg agcaggcggc agcgctcggc
    18901 gacaacaacg tggatgcgat catctacacg gtcggccacc ccaacggctc gatccaggag
    18961 gcgaccacga cggtggatgc caggctgatc ccggtcgaca cccccgagat cgcgaagctg
    19021 gtcgaggagc ggccctatta cgcctgggcg accattccgg gcggcatgta cacgggcacc
    19081 gacgaggacg tgaagacctt cggcgtcaag gcgaccttcg tgacctcggc cagcgtggat
    19141 gacgaggtca tctaccaggt ggtcaaggcc gtcttcgaca atttcgaccg gttcaagtcg
    19201 ctccatcccg ccttcgccaa cctgacggag gaggaaatgg tgacggacgg gctctcggca
    19261 ccgctgcatc cgggtgcgga gcgctactac cgcgagcgcg gctggatcca ataaggtggt
    19321 cggcacccga tggccgcttg ccatcgggtg ccgctcgctg ccaagctcca gacatgacat
    19381 gaccgaggag cggcgatgaa cgaacggtcg ggcgcggcca tcctcgagcc gcggggcaag
    19441 agcgccgccg agcttcagga catcgtcgcc gccgccgata ccggtgcccg caacccgctc
    19501 ggcccgaccg gcaagctcct cttcctcacc gcgctcgcct ggtcgctctt ccagctctgg
    19561 atcgcctcgc cgctgccgtt catgctgcgg ttcggcgtct tcaacagcac cgaggcgcgc
    19621 tcgatccatc tcgccttcgc ggtcttcctg gccttcacca gctacccggc cttcgcctcg
    19681 agcccgcgcg accatgtgcc gatcgccgac tggatcatgg cgatcctggg cgccgcctcg
    19741 gccgcctatc tctatgtctt ctatgccagc atctcgagcc gcgtcggggc gcccatcgcc
    19801 caggactatg tcgccgccgt catcggcatg ctgctgctgc tcgaggcgac ccgccgggca
    19861 ctcggcccgg cgctcgccat cgtcgccatc gtcttcctcg cctacacctt tctcgggccc
    19921 tacatgcccg gcatcatcgc gcacaagggc aactcgctcg gcgaggtggt caaccaccac
    19981 tggatcacca cctcgggcgt gttcggcatc gcggtcggcg tctcgaccag cttcgtcttc
    20041 ctcttcgtgc tgttcggggc gctcctggac aagggcgggg ccggcaacta cttcatccag
    20101 gtcgccttct cgctgatggg ccacatgcgc ggcggcccgg ccaaggcggc ggtggtctcc
    20161 tcggccatga ccggcctgat ctccggctcg tccatcgcca acgtggtcac caccggcacc
    20221 ttcaccattc ccctgatgaa gaaggtgggc ttctcgtcgg agaaggcggg ggcggtcgag
    20281 gtcgcctcct cggtcaacgg ccagatcatg ccgccggtca tgggcgccgc cgccttcctc
    20341 atggtcgagt atgtcggcat cccctatttc gacgtggtca agcacgcctt cctgccggcc
    20401 acgatctcct acatcgcgct ggtctacatc gtccatctcg aggcgctgaa ggccggcatg
    20461 gagggcctgc cgcgagctta tgagccgccg cccttgctgc gccggctgat cggtctcgtc
    20521 ttcggcgtcg ccgtggtctc ggccgtgtcg ctcgccgtct actatggcat cggctggctg
    20581 cgcccggcct tcgggccggc cgccaactgg atcgtcgcca tcggcatcgt ggtcgtctac
    20641 gtggcgctcg tcgccttcgg ggcgcgttat cccgagctcg agccggatga cccgaacgcg
    20701 ccgaccctga ccctgccctt gccgggaccg acggtgaagt cgggcctgca cttcctcctg
    20761 ccggtggtgg tgctcgtctg ggcgctcatg gtcgagcgcc tgtcgcccgg gctctcggcc
    20821 ttctacggca cggtcttcat gatcttcatc ctggtgacgc agcgcccgct cttcgccttc
    20881 ttccgccggc agggcgacct cggcggcgcg gcgcggcgcg gcttcgacga cctggtgcag
    20941 gggctcgtcg cgggtgcccg gaacatgatc ggcatcggca tcgccacggc cgcggccggc
    21001 atcatcgtcg gcgtggtcag ccagacgggc gtcggctcgg cgctggccga ggtcgtggcg
    21061 ttcctctccg gcggcagcat catcgccatc ctgctgctca ccgccgtgct ctcgctgatc
    21121 ctcggcatgg gcctgccgac gaccgccaac tacatcgtcg tctcggccct gctggccccg
    21181 gtgatcgtca cgctcggcca gcagaacggc ctcctcgtgc cgctcatcgc ggtccacctc
    21241 ttcgtcttct atttcggcat catggcggac gtgacgccac ccgtcggcct cgcctccttc
    21301 gccgccgccg cggtctcggg cggcgatccg atcaagaccg gagtcgtggc attctggtac
    21361 agcatgcgga cggcggcgct gccgttcctt ttcatcttca acccgcagct cctgctcatc
    21421 gatgtcggct gggtcgaggg catcctcgtc ttcgtcgtgg cggtcatcgc catgctgacc
    21481 ttcgccgcgg cgctccaggg ctggttcatc accaggagcc gcatctggga gagcgcgctt
    21541 ctgctgctga tcgccttctc gttcttccgg ccgggcttct ggatggacat gatctcgccg
    21601 ccctacgaga tccgcgagcc cgccgccttc accgaggtgg tcggcctggt gccgccgggc
    21661 acgctgctgc agacgcggat cgccgggctc gaccagttcg gggcacccac gcagttcgcg
    21721 acgctgctcg aggtgccgga aggggccacg ggcgaggagc gggtccaggc catgggcatc
    21781 acgctcctcg agcgcgacga caagcagatc atcgacaatg tcgccttcga cagcgcggcg
    21841 cagaaggccg gcctcgactg ggaccaggag ctgctcttct tccgcgagcc cctgccgcag
    21901 ccgagcaagt actggatttt cattcccacg ctcctggtcc tggccgggct cgtggccctg
    21961 cagcggcggc gcgtgaaggc ctgagtcgga agccccgagc ccggatcccc gagcccggat
    22021 ccccgagccc ggaaccccga aaaggagact gtccgtgttt cgcgacatcc tggtcaccgt
    22081 cgatcccgac agcccggaat cctacgcccg ggccctgccg caggcggtcg agctcgcccg
    22141 gacgcacggt gcgacgctcc acgtgatgac ggtggtgccg gatttcggct cgagcatggt
    22201 ggcgagcttc tttccggcgg gcttcgagaa gaaggcgctc ggccgcgccg aggaggcgct
    22261 cctcgagctg accggcacgc atgtgccagt ggagctcgag caccagatcg tcgtggcgca
    22321 cggccggatc tatcaggaga tctgccgcgt cgccgaggag gtgaaggccg atctcgtcgt
    22381 catggcctcg cacaagccgg ccgcccgcga caagttcgtc gcccccaatg cccagcaggt
    22441 gctgcagcgc acgtcggcct cggtgctgat cgtccgctag gaccagacca gccaggccac
    22501 gagggcgtgg atcgcgagca tcaagacgca ccaccagagg agcgccggga gggggctctc
    22561 ctttcgccgc tcgttgggca cttcgtcgcc gctcctcgac ctctgccgtc ttgcaggacg
    22621 gccagaactc aacagctgcg ccgcgatggc gcgaccgcaa tcgggatccg gcgctgtgcg
    22681 taccatcgag cgaaagcagg catgcgtggc catcgtggga atggcctcct gaaggaggtg
    22741 ctggtccgat cggggcagat gcaggcgagg cgacacggct caggcatggc ccaggcggcg
    22801 ggtcaggtga gcggcgaggc gcagggtcag catcaccagg ttgagcgtcg gatgctggct
    22861 gccggacgtc gggaagaccg agctgccggc aatgtagagg tcggtcatgc cgtgcacccg
    22921 gcagtccggg tcgaccacgc ccgttcgcgg gctgtggctc atgcgcgtgg cacccatgtg
    22981 atgcgcgctg ttaccgtgcg gcgggaaggg caggttctcg tcgatcaccc agggggcgag
    23041 cacagcccgg ccgaggccgc gcgcggcgag gtggtcggcg aggtggcggg cgaagcggcg
    23101 gaccgttcgt cggtcggtct cgccgaggcg ccagtcgacc ctggcgcgcg gtcggccgaa
    23161 cgcatcgacc gcttgatcga gggtgatccg gctcgccggg tcagggcact gctcgatgag
    23221 cgtgctcacg gtcagccggc cggtctcggg tgccagccag ccgagcttga ccagcgattc
    23281 gtggacgagg ccgggcagat cgccgagcat ttccccgccg tcccggatca gcgaggcatc
    23341 gagctggccg tggccgagct tgcgctgcag ccgccgggcg gcccgaaagc cgggcggccc
    23401 gaactggccg tcgctgtaga aggccgcgtc gacggcggtt gcttccagcg cctcctgccc
    23461 ggcccggttg agatgcagga gcccctcgat gtggccgtgc gccggcgtgt cccggtcatg
    23521 cagcagttcc ggcaggcggg cgccctcggc cagcgacagc tgcagcggct cgaagatcgg
    23581 atggcccatg aagtggcggc cgacctggtc gtgccggttg ccgatgccgg ccgcgagctg
    23641 gcggcggcag gcgagcagga ggcgggcgtt ctcgatcccg cccatggcca gcacggtgac
    23701 cttcggccgc aagcgcaggg tcttgccggg ccggctcctg aggtccagcc attggatccg
    23761 gtcgagcgag ggggcgagct cgatgtcgac gagatcggcg tcgagcagca gcgtcaggtc
    23821 ggccctcgcg gcgagctcgg tccgccagcg ctcgcccatg cgggtcggcg ggctgaagcg
    23881 ccagacggcc ggggtgagcg tcggcccgcc gagggcctgg gccgcggcgt cgctgtcggc
    23941 cggcgcgtcc cagggctcgt ccggcggccg gtcgatctcg agcagccggc aggcggcagc
    24001 gaggtggcgg cgcagctcgc ccgcctcgag cggccaggcg ggcaggccga gccagggccg
    24061 cgccgcgagc gcctcggcgt cgagcggccg cgagcggccg ccccagtggt tggtcgagcc
    24121 gccgagctgg cgcagtctcg tggtctcggg gtcgagcggc aggttgccga tggagctgcc
    24181 ggcacagaag gactggtgcc acggctcgaa cgcctcgtcg ccggcctcgg cgaggacgat
    24241 ctccaggccg ctcccggcga ggccgagtgc gaggccgagc ccggccgcac cgccaccgac
    24301 gatggcgaga tcgcaggggc gatcgccacc ggtttcgagg gaatcgaggg cgacgagcat
    24361 ggcgcgtgga ccgggtgcgg ggctggaatc cctgcccaac aagatagaat gaaattagcc
    24421 cataatcaac tataaggcta gagcgtcagc ggggtctccc gcgcgatcag cccggccgtg
    24481 gccagctcct gccagagcgc cggcgggatc ggcagggcca tcagcgcgag gttccgttcg
    24541 acctccgtcg ccgagcggca gccggtgacg acgctcgcga tggccggatg cgaggcggag
    24601 aactgcaggg cggcggcggc gagcgggacg ttgtgggcgg cacagatggc ctcgatcctg
    24661 cttgtgcgct cgacgatttc ggtgtcggcc ggggcgtaga agaaggtggc gccggccttc
    24721 gcaccggtgg cgaggatgcc cgagttgaag gggccggcga ggaggacgcc gatgccctcg
    24781 gcggcgcagt aggggaggaa gtcctt
//
LOCUS       DAOTFI010000034        24716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2365_length_24716_cov_7.453307, whole genome shotgun sequence.
ACCESSION   DAOTFI010000034 DAOTFI010000000
VERSION     DAOTFI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 24716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24716
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2365_length_24716_cov_7.453307"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..990)
                     /locus_tag="P5558_05065"
     CDS             complement(67..990)
                     /locus_tag="P5558_05065"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23711.1"
                     /translation="MPDRPILVTGAQGLIGNAARAALEDAGRPVVAVDREAGEVAGRP
                     VLAGDVTAVHRLHALAVEHGGFDGILHCGAFSGPMVAADDPAAMVAVNITGTANMLEL
                     ARIHRVRRLVFCSSVSAYGDTPDGLDPVPEATPLHPTSVYGASKAAGEALVDGYAAQF
                     GVDGLALRIGWVYGPRRATACAIRDMLQHARAGQPFHLPFGADHHRQYVHVDDVARAL
                     VLALDAERPGQRAYNINGGSRVTLGEIAGLVGRIVPGASLTLEPGPDPLDDRQAQFDL
                     RAAERDLGFRPAIGLDEGIRRYAAWLAGTAA"
     gene            complement(1001..2011)
                     /locus_tag="P5558_05070"
     CDS             complement(1001..2011)
                     /locus_tag="P5558_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY23712.1"
                     /translation="MNYRNLGRSGLQVSPLCLGTMMFGGRTDAETSNQIIDMARSAGI
                     NFIDTADVYNGGESERITGAAIAGDRHAWVLATKLANPMGDGPNSRGLSRKWVIEACE
                     ASLRRLGTDFIDVYYLHKEDHRTPLDETVRAVADLIRAGKVRYLGLSNYRAWRIAEIC
                     RLCDAAGIDRPAVLQPYYNMLNRQPEVELLPASGHFGLGVAPYSPLARGVLTGKYDPD
                     GPPSEDSRAGRQDTRMMQTEWRKESLVIARKLQAHADAKGCTPGQFAVAWLLNNRLVT
                     SPIAGPRTIAQFEDYLGALDVAFTAEDEALVESLVTTGHASTPGYNDPAYPIEGRRPV
                     AG"
     gene            complement(2023..2799)
                     /locus_tag="P5558_05075"
     CDS             complement(2023..2799)
                     /locus_tag="P5558_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRY23713.1"
                     /translation="MKVGVIGGGGGIAASLQAAGVAVVGCAAGELAPDHGVERVEAYQ
                     DFLEALDYPRRYVLDMPPGAAIDAVIDEAYQTMEPGDVVIDATASYWGDTLRRYRRMR
                     HRSLFYQDIARLAGPDGAALVVSGDAKGLKLTADLLEAWARPDGYRPVAGAGFAHYLA
                     MLEAAATAARRQLAGELNQLVEAYPGEVEEAAKAAFCTDLGPLDLGRAGWLLDDAVRL
                     EAAVPLTAQALMLTVGEALEAHRSTPPPPRGAFVHPDDIL"
     gene            complement(2892..3083)
                     /locus_tag="P5558_05080"
     CDS             complement(2892..3083)
                     /locus_tag="P5558_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23714.1"
                     /translation="MRIAVIACAAAIGLAAGPVAAECNWSATAQAETSKVVAAATSTT
                     EAAPAQSTPVRLPETDEQG"
     gene            complement(3165..3338)
                     /locus_tag="P5558_05085"
     CDS             complement(3165..3338)
                     /locus_tag="P5558_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23715.1"
                     /translation="MRVVVLIGSLGLLTLAACTARVTPPKIEVEAKPGVEIDVGSDTG
                     KFCPPGQAKKGRC"
     gene            complement(3415..4494)
                     /locus_tag="P5558_05090"
     CDS             complement(3415..4494)
                     /locus_tag="P5558_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY23716.1"
                     /translation="MTPRLTRRHLLGTAASGAALLTAPRLGRTQMDKVRAGFVYVGPI
                     GDHGWTYRHNVGRLDLEAALGDKVETTFVENVAEGPDAERVIRQLATGNDIVYTTSFG
                     FMNPTVRVARQFPAVKFEHCTGYQTTENLAVYNARFYEGRAVIGTIAGHMSKSGVVGY
                     IGSFPIPEVVMGINAFQIAMRKVRPDCEVRVVWVNSWYDPGKEADAARTLIDQGADII
                     SQHTDSPAPLQVAEERGVLGFGQASDMTAFAPNAQLTAIVDNWGPHYIQRTQAVLDGT
                     WATQNVWHGLKDGEVEIAPYNPSVPADVIEAAEAVRLGIIDGRVHPFAGPIRNQAGEV
                     VVAEGATLSDEDLLKMDWYVEGVQA"
     gene            complement(4592..>5014)
                     /locus_tag="P5558_05095"
     CDS             complement(4592..>5014)
                     /locus_tag="P5558_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23717.1"
                     /translation="AVGENHEAAHGLGYPVIRIRYLAVLFGGLAAGLGGAFLSLAYTP
                     MWIEQMTAGRGWIALALVVFAAWRPLRVLVGAYLFGGVTILQLHAQGLGLFAVPSQVL
                     SMLPYLATIVVLVVISVGPWKGRLQAPACLGRPFHPTH"
     assembly_gap    5015..5114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5187..6260)
                     /locus_tag="P5558_05100"
     CDS             complement(5187..6260)
                     /locus_tag="P5558_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23718.1"
                     /translation="MVRLEPRAAISRKMRWLTPLLAVALTVLTGAVLFAAMGYDPIRA
                     LRAFFVDPLTSRYGLAELGVKATPLILIATGLAVGFRAGVWNIGAEGQLTLGALAGGS
                     VALLFWNQEGWWILPLMGIAGVAGGMAWAAIPAFLKARFDVNEILVSLMLTYVGLLLL
                     SALIYGPLKSPEGFGFPESRMFSESALLPGILAGTRLNLGMPITVLVVLVVWFLLART
                     LFGFQVTVIGQAPAAARFAGFSQKAMIWTSLLLGGGLAGLAGLFEVAGPIGQIVPTIS
                     PGYGFTAIIVAFLGRLHPFGIVLAGLLVALSYLGGENAQITVGMPQAVTGVFQGMLLF
                     FLLAADVLVRYRLRFGSPAPVRS"
     gene            complement(6253..7806)
                     /locus_tag="P5558_05105"
     CDS             complement(6253..7806)
                     /locus_tag="P5558_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23719.1"
                     /translation="MTVARLVLSGIKKAYPGALANDDVALTVGQGEIHALLGENGAGK
                     STLVKIIYGVLAPDAGEIAWEGRPVTIPSPHAARTLGIGMVFQHFSLFDALTVLENVA
                     LGLDAKLDRRTLAERITKVSQAYGLPLDPHREVHELSVGERQRIEIVRCLLQSPRLLI
                     MDEPTSVLTPQEVERLFSTLRQLAKEGVSILYISHKLHEIEALCDAATIMRGGKVVAH
                     CDPRTETARSMAELMVGSSFAAPEKPTRRLKRAPRFSVNALDLPTAEVFGTALREISF
                     DVQVGEIFGIAGVAGNGQSELLAALSGERLTEPGFVVIDGQKVGRLGPRARRMHGLAY
                     IPEERNGHAAVPDLTLAGNALLSAFDRRGLLSSGVIRHARASAFAQEIIDRFSVRATG
                     PTARASALSGGNLQKFVVGREILQAPNVLIAAQPTWGVDAGAAAAIHQALIDLAGHGV
                     AVLVVSQDLDELMALTDRMAVLNGGRLSRPLVTAEATVEQIGLLMGGLHDLPDEVPQG
                     DGQPETTHG"
     gene            complement(7834..8658)
                     /gene="xdhC"
                     /locus_tag="P5558_05110"
     CDS             complement(7834..8658)
                     /gene="xdhC"
                     /locus_tag="P5558_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HRY23720.1"
                     /translation="MTEALVAALATIDQSEPAILVEIVEALGSTPREAGALMLVTGDS
                     IAGTIGGGELEHRAILRAREALAGEPVPERLDLPLGPALAQCCGGHVTLRLRHLAPGD
                     RTMLAERLGAWQAGLPRVVLFGAGHVGRAVVAALAPLPCRVLWLDSRPGQLPADASGL
                     VETREIADPAGFATAPGDLVLVMTHSHPLDLAIVEAVLRRGSFAWLGLIGSATKRRRF
                     ERQLGAVGLSVERLVCPIGLAAITGKAPAVIAASVTAQLLVVMEALTRGRQIRSVA"
     gene            complement(8655..10955)
                     /gene="xdhB"
                     /locus_tag="P5558_05115"
     CDS             complement(8655..10955)
                     /gene="xdhB"
                     /locus_tag="P5558_05115"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HRY23721.1"
                     /translation="MSEASPIGRALAHDSALRHVTGEAVYVDDMPEPARCLEIYIAWA
                     ERAHARVKRLDLAAVRAAPGVVAVLTNDDIPGVNDISPVGRDDEPVFATGIVQYWGHA
                     LFAVAAPTVHAARAAAALASIEYEDLPPLLDVDEARAREDHVWPPGRMQRGDAVRALA
                     ASPCRLDGRIAIGGQDHFYLEGHVALALPGEDGEMMVYSSTQHPSEVQHMVAHVLGVP
                     SHSVTVEVRRMGGGFGGKETQGNLFACIAALAARRTGRPAKCRLDRDDDMIMTGKRHE
                     FLVDYAVGCEPDGRISAVDMTFASRCGYSADLSGPINDRALFHADNCYFYPDVALVCH
                     PCRTNMVSATAFRGFGGPQGMVAAERVIEEVAIRTGLDALEVRKRNFYGPNERAVTPY
                     WMTIEDNVIERVVAECEKDAGYGARRAAIGRFNTDNRWQKKGLALTPVKFGISFTNTA
                     FNQAGALIHIYKDGSVHLNHGGTEMGQGLFIKVAQVVAEELALPLERIKITATTTGKV
                     PNTSATAASSGSDLNGMAAQAAARELKARLVAFAAEQHQMPADAVDWLPGRVRVAGDE
                     ITFDRLVHDAYMARVQLSATGFYKTPEIHWDRATGRGKPFFYFAYGAACSEVTIDTLT
                     GEYRVDRVDIVHDVGRSLNPAIDMGQIEGGFIQGMGWLTTEELVWDKEGRLRTHAPST
                     YKIPTCADRPEIFNIRLLENVNPKATIHRSKAVGEPPLMLAISVWQALSDAVAATAGH
                     KHPARLDPPATPEKVLMAIDRLAGLA"
     gene            complement(10948..12423)
                     /gene="xdhA"
                     /locus_tag="P5558_05120"
     CDS             complement(10948..12423)
                     /gene="xdhA"
                     /locus_tag="P5558_05120"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HRY23722.1"
                     /translation="MRQRLRFLLGHEPVELEAIDPTLTVLEWLRRVAYRRGTKEGCAE
                     GDCGACSVVIAEPEDGRLRYRAINACIRFMATLDGCQLLTVEDLKAPDGTLHPVQAAM
                     VDSHGSQCGFCTPGFVMTLFALHQNGDTALSRGRIDDALAGNLCRCTGYGPIIEAARR
                     MRETPGAVAHLAHREAEVLARLDSWRDGETLAIGEGGRMIYAPTELDALLDLAADHPQ
                     ATILAGATDVGLWVTKQQRRLDPVIWLGRVAGLARIEETEDAIHIGAGVTYAAAHERL
                     AGLWPDVGELVRRLGAEQIRHAGTIGGNIANGSPIGDSPPWLIAAGATLHLAHRDGRR
                     SLPLEEFFLAYGRQDRRPGEIVEAVSVPKPEAGTELRCYKISKRFDQDISAVCAGIQL
                     RRDGDRIVEARIAYGGMAATPRRALRAEAALIGQPFAEATFRRAMAALAADFQPIDDM
                     RASAAYRLKVAQNLLLKAYLEAEDATSPSLRLAGRREPVHV"
     gene            complement(12423..12776)
                     /gene="uraH"
                     /locus_tag="P5558_05125"
     CDS             complement(12423..12776)
                     /gene="uraH"
                     /locus_tag="P5558_05125"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRY23723.1"
                     /translation="MGKLTTHVLDTMSGRPGHGMQLALYRVDGADRVKLAEARTNEQG
                     RLDRPMLEGASMGRGTFELIFQVGHYFKLLGVDLPNRPFLDEVPIRFGIEDPGADYHV
                     PLVVSPFAYSTYRGS"
     gene            complement(12853..14016)
                     /locus_tag="P5558_05130"
     CDS             complement(12853..14016)
                     /locus_tag="P5558_05130"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRY23724.1"
                     /translation="MPSRVAAGLTFTLVLCLSLPALSADAPVAASALAPSEFVVTRPG
                     LVLVDVAARRAVLEALPEAEREALCGPPRQSWPRHGVQRNVGGGAQPAQTLALTMMHA
                     AASYLATGDPDARDGIISNLLRFAEADALSRMTEPVTVNHFYNLDRTLLPIVTGLAMV
                     HDDPALAPDEVATIRAWLDRLVRRRGPDRVTNPEVASSRNNHRYLRDSVTMAWGAFTG
                     DNALFFEGVNRYRLALEQMREDGSLPLETDRGRDALFYQRHAIASLVAMAEIAAVQGY
                     DLYALTSEAGLDLHQMVAFLVAGIDDPAVLAPYTKAPQDLGFLDWRGHDRHYMAWFEA
                     YRARFPESPLTARLAAQIEARGSLDGQLVDEYAGGMTTCLYADPMTRVAISAE"
     gene            14100..14867
                     /locus_tag="P5558_05135"
     CDS             14100..14867
                     /locus_tag="P5558_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23725.1"
                     /translation="MKSPFDLGGKVALVTGGNGGIGLGMARGLGRAGAAVAVAGRNQA
                     KTAAAAAELTSEGVRAIAVVGEVADPAACVAMVEEVEAELGRLDIAVCNAGINIRKRP
                     EAYEFAEWREVLAINLDGVFNTAHAAYPALCRQGGGKIITTGSMMSIFGIPVVAAYAA
                     SKGGVMQLSRALATAWARDNIQVNCILPGWIDTDLTRRGRAEIEGLNEKVLTRTPAGR
                     WGTPEDMAGLTVFLASPASDFITGTAIPADGGYSIQG"
     gene            complement(14872..15450)
                     /locus_tag="P5558_05140"
     CDS             complement(14872..15450)
                     /locus_tag="P5558_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23726.1"
                     /translation="MVVPSRPRFTLIVVTSRDGYIAPEGGAPPGVWASREEQRLFRHR
                     VAGLDWTFMGRRTHELAWRPNRRRVVFTAALAGPEWRHPLHLWADPGRVSLDAMLAAA
                     TAVHPAVSCGILGGVAVHDWFAAAGLIDAVELTIEPLTFAGGLPLFSWSRGMKPCHAL
                     SGLGLLQVEEQPLNAGGTRLLRFERPSGPIGP"
     gene            complement(15510..15653)
                     /locus_tag="P5558_05145"
     CDS             complement(15510..15653)
                     /locus_tag="P5558_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23727.1"
                     /translation="MRTVSTRRGARRVPVSSANGTPPQLVIVAASLALWTIIIASFSL
                     LLG"
     gene            complement(15873..16430)
                     /locus_tag="P5558_05150"
     CDS             complement(15873..16430)
                     /locus_tag="P5558_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRY23728.1"
                     /translation="MNPTLTTALWPSSAQSLPARIVLVLAGTLLLAVSARVQVPFWPV
                     PMTMQTFVVLGIGAAYGARLAGITLLTYLAQGAMGLPVFATGAGLAYLAGPTGGYLVG
                     FFVAAVVVGYLADEGLGRTHLSAFFVFLAATLLIFGLGATWLALLIGPAKAFAAGVLP
                     FLPAEAAKLLLATAFFPIAWRLARD"
     gene            complement(16462..17436)
                     /locus_tag="P5558_05155"
     CDS             complement(16462..17436)
                     /locus_tag="P5558_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRY23729.1"
                     /translation="MAIEVEAPGLATTVQDLGRPGYYHLGIPLSGAMDRLALRAANRL
                     VGNDDGAAALEVVFLGPELVFRADAMVAVTGAELPPRVDGVVQPTWTSFAVKAGQTLA
                     FDYLKAGARAYVAVAGGIDVPVVLGSRSTYALGALGGHEGRALRAGDVLPVGRGRPAA
                     AGREIPAALRRGPGGSPVSLRVLPGLYWHRLTEAAGRQFLADTWKVAPEADRIGYRFR
                     GGRPLEFMPHEQPFGAGSDPSNIVDACYPYGSIQVPGGTEPIVLHRDAVSGGGYMMVG
                     TVISADMDLIGQLQPHTPVRFVAVDMAGALAARHDRQAILDKMQSHLA"
     gene            complement(17426..18304)
                     /locus_tag="P5558_05160"
     CDS             complement(17426..18304)
                     /locus_tag="P5558_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HRY23730.1"
                     /translation="MTNRYSFGGDEHIFVEVDESMSLEAFFKALSITNAVRESRIDGV
                     TEICPANASFQIKFDPDRIKPDDMLAELKRLEAAAAQAPSVIETRIFEFPVYYNDPWT
                     HETLMRFRDRHQDPSATDLDYAARINNKASVEEFIAAHSGAPWFVSMVGFVAGLPFLF
                     QMVERARQLEVPKYLRPRTDTPKLTVGYGGCFTAIYSVRGAGGYQMFGITPVPIYDPK
                     SEIDYISDDTMCLFRPGDIVKFKSVDRAYYDHVVEEVAAGRYAPKVRNVTFSLDAFNA
                     DIDGYNRKLEEVLDGH"
     gene            complement(18301..19701)
                     /locus_tag="P5558_05165"
     CDS             complement(18301..19701)
                     /locus_tag="P5558_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRY23731.1"
                     /translation="MREVRLLVANRGEIAVRIIRAAKELGFVTIQAHSRADADSLAVR
                     LADEAVDIGPPQASKSYLKIEALLEAAKSTGATAVHPGYGFLAENADFADAVEAAGLA
                     FIGPTGDAIRLMGDKVAARQAAAAAGVPTVPGSEGHVRTLHEARPVVARTGFPLMIKA
                     AAGGGGRGIRIVRDEAEFERLLPQASAEAKAAFGDGGLYLEKVVERARHVEVQILGDG
                     QHVVHLFERECSLQRRRQKVWEEAPSAGLSAAVRRKLCASAVALARAVNYRGAGTLEY
                     LYDDQSHDFYFIEMNTRIQVEHPVTEMVTGIDLVREQLRVCLGQKLRHTQDQIRLHGH
                     AIEVRLNAEDPARDFAPGPGTIDRLSLPGGPGVRFDSLLYAGYTVPPFYDSLLGKLIV
                     WDEDRSHALARLARALGELEIGGVATTRALHLALAADPDVRAARYHTTWLERWLEENA
                     ARLAPATDRTGEVPTA"
     gene            complement(19711..19953)
                     /locus_tag="P5558_05170"
     CDS             complement(19711..19953)
                     /locus_tag="P5558_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HRY23732.1"
                     /translation="MASKQILSPLPGTFYRRPAPDQPPFKSEGDTVEVGDVVGLVEVM
                     KSFHEVKADAAGTLGAFLIDDEDAVMAGQAIADLEG"
     gene            complement(20027..20695)
                     /locus_tag="P5558_05175"
     CDS             complement(20027..20695)
                     /locus_tag="P5558_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY23733.1"
                     /translation="MTRPPAEPVTHLDDGRFRVTEWRFAPGAETGWHVHGHDYVVVPL
                     VDGTLLLEEPGGTSRKASLTRHVPYARRTGVEHNVVNDASEAMAFLEVEVLGDALQAA
                     RLACLERFAAAWNAHDVDALMACMAEDCAFHASAGPAADGRRHAGGDAVRAAYAAIFE
                     QFPKAAWTQGEHRVAGDHGVSSWRFVGTDRQGATVEVDGCDLFTFAGERIALKDSYRK
                     SRTA"
     gene            complement(20757..20832)
                     /locus_tag="P5558_05180"
     tRNA            complement(20757..20832)
                     /locus_tag="P5558_05180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20797..20799),aa:Ala,seq:cgc)
     gene            21003..21284
                     /locus_tag="P5558_05185"
     CDS             21003..21284
                     /locus_tag="P5558_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRY23734.1"
                     /translation="MIAVYNLINTVISIYIWLLIAMVVLNLLVSFNMVNTGNRFIYTI
                     GDFLNRITEPLLRPIRNILPNLGGIDISPMVLILALYFIRDLAFQFLVL"
     gene            21285..21611
                     /locus_tag="P5558_05190"
     CDS             21285..21611
                     /locus_tag="P5558_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HRY23735.1"
                     /translation="MTPPWRLEPDRAILEIRAVPGARHERVDGIAMDARDRPHLVIRL
                     RAPAIEGKANAALLAFLAKRLDCPRSRLELVAGATGRLKRVLWKDPPTDAARRLAALH
                     PAPGDG"
     gene            21711..22430
                     /gene="rph"
                     /locus_tag="P5558_05195"
     CDS             21711..22430
                     /gene="rph"
                     /locus_tag="P5558_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRY23736.1"
                     /translation="MRASGRAADELREVVLEPGANPQAEGSCLVRFGHTHVLCCATVE
                     ERVPPFLRNRGTGWVTAEYGMLPRSTHSRSPREAARGKQGGRTLEIQRLIGRALRAVT
                     DLEGMGERQIIVDCDVLRADGGTRTASITGAYVALHQALAKLVRDGALPALPLTDAVA
                     AVSCGIVGGEALLDLDYPEDSAAEADANFVLAGKGGIVEIQFTAEKEPLPRQDLARLM
                     TLAEGGIEQLLALQRAAVHAG"
     gene            22438..23052
                     /gene="rdgB"
                     /locus_tag="P5558_05200"
     CDS             22438..23052
                     /gene="rdgB"
                     /locus_tag="P5558_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRY23737.1"
                     /translation="MHRRFTGDTLVLASHNPGKLQELQELLADHGVKVRSAGELGLIE
                     PEETEPDFQGNAALKAHAAARATGLPALADDSGLEVMGLEGRPGVHSARWAGPGKDFA
                     AAMTRVTSELAERFGDWQRVDKRAAFVSVLCLAWPDGHAEVFEGRVSGELLPEPRGAG
                     GFGYDPIFVPEGEKRTFAELSRTEKSALSHRGIAMRALLDAVFR"
     gene            23102..23177
                     /locus_tag="P5558_05205"
     tRNA            23102..23177
                     /locus_tag="P5558_05205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23135..23137,aa:Ala,seq:tgc)
     gene            complement(23228..23821)
                     /gene="pyrE"
                     /locus_tag="P5558_05210"
     CDS             complement(23228..23821)
                     /gene="pyrE"
                     /locus_tag="P5558_05210"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRY23738.1"
                     /translation="MSLTTDAVLDEFRAAGALLEGHFLLTSGRRSPFYLQCARVLMDP
                     ARAERLCTALAARVREAQAATTIDAVVAPAMGGILVGYELARALGCLSLFTERVEGRF
                     TLRRGFALEPGARVLIAEDVVTTGLSLRECADAVRATGAVVVGAAALIDRSNGKADIG
                     MKLTALATVDFPTYAPDEVPADLAAIPAIKPGSRGLA"
     gene            complement(23818..>24716)
                     /locus_tag="P5558_05215"
     CDS             complement(23818..>24716)
                     /locus_tag="P5558_05215"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HRY23739.1"
                     /translation="MVQLRHTLENGDQVEIITSRNAWPTAAWERYVVTGKARARIRRA
                     LKARQRDDYVTRGREMLARTARAEAMQPTEKQLAQAATELGHASLEDMLVQVGEGQLA
                     ARLVLQAIPAPALRLPARDGRVAERERGEDLDNIVLFKRPPAESDEGRSGVIAGLPSG
                     IAYQFAGCCRPIPGDPIVGIVRTGRPISIHCRDCATVGRMGESEHRFVDIGWSGQGEP
                     PLASTRISVMVTNQPGALGTISTIIGKQEGNIVDVKVGRRSPDLFEMLLDVEVRDVDQ
                     LQRIQAALRANPIVSTVERSRS"
BASE COUNT         3776 a         8502 c         8829 g         3509 t
ORIGIN      
        1 agggccggtg cagcgagggc cacgttaccg agcgccagcc cgcggagcaa cgcccgacgt
       61 cgcatctcag gcagccgttc cggcgagcca ggcggcatag cgcctgatcc cctcgtcgag
      121 cccgatcgcc gggcgaaagc cgaggtcgcg ttcggcggcg cggaggtcga attgtgcctg
      181 gcgatcgtcg agcgggtccg gcccgggctc gagcgtcagg gaggcgcccg gcacgatccg
      241 gccgaccagg ccggcgatct cgccgagcgt cacgcggctg ccgccattga tgttgtaggc
      301 gcgctggccc ggccgctcgg cgtcgagcgc caggacgagc gcccgggcca cgtcgtccac
      361 atggacatac tggcgatggt ggtcggcgcc gaacggcagg tggaagggtt gccccgcccg
      421 ggcatgctgc agcatgtcgc ggatggcgca ggcggtcgcc cggcgcgggc catagaccca
      481 gccgatccgc agggcgaggc cgtcgacgcc gaactgcgcg gcgtagccgt cgaccagcgc
      541 ctcgcccgcg gccttgctgg cgccatagac actggtgggg tgcaagggcg tcgcctcggg
      601 caccgggtcg aggccatcgg gcgtgtcgcc ataggcgctg accgacgagc agaagacgag
      661 gcgccgcacc cggtgaatgc gggccagctc cagcatgttg gcggtgccgg tgatgttgac
      721 ggcgaccatc gctgccggat cgtcggccgc gaccatgggg ccggagaacg ccccgcaatg
      781 caggatgccg tcgaagccgc catgctccac tgcaagggcg tgcagccggt gcacggcggt
      841 gacatcgccg gcgagcaccg ggcgcccggc cacctcgccg gcctcgcgat cgaccgcgac
      901 gaccggccgg cccgcatcct cgagcgctgc ccgggcagcg ttgccgatca ggccctgggc
      961 gccggtgacg aggatgggac ggtcgggcat ccctgagctc ctagccggct accggccggc
     1021 gaccctcgat cgggtaggcc gggtcgttgt agccgggcgt gctggcatgg cccgtcgtca
     1081 ccaggctctc gaccagcgcc tcgtcctcgg cggtgaaggc gacgtcgagc gcccccagat
     1141 agtcctcgaa ctgggcgatc gtccgggggc cggcgatcgg gctcgtgacg agacggttgt
     1201 tgagcagcca ggcgaccgcg aactggcccg gcgtgcagcc cttcgcgtcg gcatgggcct
     1261 ggagctttcg cgcgatcacc aggctctcct tgcgccattc ggtctgcatc atgcgcgtgt
     1321 cctgccggcc ggcccggctg tcctcggacg gcggcccgtc cggatcgtac ttgccggtga
     1381 gcacgccccg ggcgagcggg ctgtacggcg ccacgccgag gccgaaatgg cccgaggccg
     1441 gcagcagctc cacctcgggc tggcggttca gcatgttgta gtagggctgc agcacggccg
     1501 gccgatcgat gccggcggcg tcgcagagcc ggcagatctc ggcgatccgc caggcacggt
     1561 agttggacag gccgagatag cggaccttgc cggcgcggat caggtcggcc accgcgcgca
     1621 cggtctcgtc gagcggggtc cggtgatcct ccttgtggag gtagtagaca tcgatgaagt
     1681 cggtgccgag ccggcgcagg ctcgcctcgc aggcctcgat gacccacttg cggctgaggc
     1741 cgcgactgtt cgggccgtcg cccatcgggt tggcgagctt ggtggcgaga acccaggcgt
     1801 gccggtcgcc ggcgatcgcg gcaccggtga tccgctcgct ctcgccgccg ttgtagacgt
     1861 cggcggtgtc gatgaagttg atcccggccg agcgcgccat gtcgatgatc tggttcgagg
     1921 tttcggcatc ggtccggccg ccgaacatca tcgtgccgag gcagagcggc gagacctgca
     1981 ggccggagcg gccgagattc ctgtaattca tgattcctgc tttcaaagta tgtcgtcggg
     2041 atggacgaag gcgccgcgcg gcggcggcgg ggtgctccgg tgcgcctcga gcgcctcgcc
     2101 gacggtgagc atcagcgcct gggcggtgag cggcacggct gcctcgagcc gcaccgcatc
     2161 gtcgagcagc cagccggcgc ggccgaggtc gagtggtccg aggtcggtgc agaaggcggc
     2221 cttcgcggct tcctccacct cgcccggata ggcttcgacg agctggttga gctcgccggc
     2281 gagctggcgg cgggcagcgg tcgccgcggc ttcgagcatg gcgaggtagt gggcgaagcc
     2341 ggcaccggcg accggccgat atccgtccgg tcgcgcccag gcctcgagca gatccgcggt
     2401 cagcttcagc cccttggcgt cgcccgagac gacgagggca gccccatcgg ggccagcgag
     2461 gcgggcgatg tcctggtaga agagcgagcg gtggcgcatg cggcgatagc ggcggagcgt
     2521 gtcgccccag tagctcgccg tcgcgtcgat cacgacgtcg cccggctcca tcgtctggta
     2581 ggcctcgtcg atgacggcat cgatggcggc acccggtggc atgtccagca cgtagcgacg
     2641 cgggtagtcg agcgcttcca ggaaatcctg gtaggcttcg acccgctcga caccgtggtc
     2701 cggagcaagc tcacccgcgg cgcagccgac aaccgcgacg cccgcggcct gcaggctggc
     2761 agcgatgccg ccacccccac cgatgacacc gaccttcatt cccgatgctc ccgtcatcgc
     2821 tcacatcggg aaagaacggg ccgactgggc cggcccgtgt caagcgtgac ccgaggaccg
     2881 atcgacgcgg gtcagccctg ctcgtcggtc tccggcagac ggaccggcgt gctctgcgcc
     2941 ggagccgcct cggtcgtcga ggtggccgca gccacgacct tcgacgtctc ggcctgggcc
     3001 gtggcgctcc agttgcactc ggcggctacc gggccggcgg cgaggccgat ggcggcggca
     3061 caagcgatga cagcgatgcg catggcaact gttctcctgg ctggtggaca ccagtcagaa
     3121 ttgacccgat tcccggccaa ggcaagcgcg gccggccagg ttcctcagca gcgacccttc
     3181 ttcgcctgac ccggcgggca gaacttgccc gtgtcgctgc ccacatcgat ctcgacgccc
     3241 ggcttcgcct cgacctcgat cttcggcggg gtgacgcgcg cggtgcaggc ggcgagggtc
     3301 aagagaccga gcgaaccgat caggacaacc acgcgcatcg accaggactc ccgagctgca
     3361 tcccgcccat catgcgcggg aagcagtcag gaaaccgtta atggcaacac ctgatcaggc
     3421 ctgcacgccc tcgacgtacc agtccatctt caggagatcc tcgtcgctca gcgtcgcgcc
     3481 ctcggcgacg acgacctcgc cggcctggtt cctgatcggc ccggcgaagg ggtggaccct
     3541 gccgtcgatg atgccgaggc gcacggcctc ggcggcctcg atgacgtccg ccggcacgct
     3601 cgggttgtag ggggcgatct cgacctcgcc atccttcagc ccgtgccaga cgttctgcgt
     3661 cgcccaggtg ccgtcgagca ccgcctgggt gcgctggatg tagtgggggc cccagttgtc
     3721 gacgatggcg gtgagctgcg cgttgggcgc gaaggctgtc atgtcggatg cctggccgaa
     3781 gccgaggacg ccgcgctcct cggcgacctg gagcggtgcc gggctgtcgg tatgctggct
     3841 gatgatgtcg gcgccctggt cgatcagcgt gcgggcggcg tcggcctcct tgcccgggtc
     3901 gtaccaggaa ttgacccaga cgacgcggac ctcgcagtcg gggcgcacct tgcgcatggc
     3961 gatctggaag gcattgatgc ccatcacgac ctcggggatc gggaaggagc cgatatagcc
     4021 gacgacgccg gatttcgaca tgtggccggc gatcgtgccg atcaccgccc ggccctcgta
     4081 gaagcgggcg ttgtagacgg cgaggttctc cgtcgtctgg tagccggtgc agtgctcgaa
     4141 cttgacggcg gggaattgcc gggcgacccg cacggtgggg ttcatgaagc cgaaggaggt
     4201 ggtgtagacg atgtcgttgc cggtggcgag ctggcggatg acccgttcgg catccggccc
     4261 ctcggcgacg ttctcgacga aggtcgtctc gaccttgtcg ccgagcgccg cctcgagatc
     4321 gagtcgcccg acattgtggc gataggtcca gccgtggtcg ccgatggggc cgacatagac
     4381 gaagccggcc cggaccttgt ccatctgggt gcggccaagg cggggtgccg tcagcagcgc
     4441 ggcacccgag gcggcggtgc cgagcagatg gcggcgagtg aggcgaggcg tcatcgggag
     4501 gtctccattc agtcgagcca agcggcgtgt cgagcggagc aatagcgacc ggctttcggc
     4561 cttggcaagc gccgccgccg ggttcggctg gtcagtgcgt cgggtggaag ggccggccga
     4621 ggcaggccgg cgcctggaga cggcctttcc agggtccgac cgagatgacg acgagcacca
     4681 cgatggtggc gaggtagggc agcatcgaca gcacctggct cggcacggcg aagaggccga
     4741 ggccttgcgc atggagctgg aggatggtga cgcccccgaa gagataggcg ccgacgagga
     4801 cccgaagcgg gcgccacgcg gcgaagacga cgagcgccag cgcgatccag ccccggcccg
     4861 cggtcatctg ctcgatccac atgggcgtgt aggcgagcga gaggaaggcg ccgccgagcc
     4921 cggcggcgag cccgccgaag agcaccgcca gatagcggat gcggatcacc ggatagccca
     4981 ggccgtgggc ggcctcgtgg ttctcgccga ccgcnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnggcggc gaagaggagc ggtgtcgcgg cggtgacgac ggtgacgagg
     5161 agggcggtga ccgggtccat gggctctcag ctcctcaccg gtgccgggct gccgaagcgc
     5221 agccggtagc gcaccagcac gtcggcggcg agcaggaaga agagcagcat gccctggaac
     5281 acgccggtga ccgcctgcgg catgccgacc gtgatctgcg cgttttcgcc gcccagatag
     5341 gatagcgcca cgaggagccc ggcgaggacg atgccgaacg ggtgcaggcg gccgaggaag
     5401 gccacgatga tcgcggtgaa gccgtagccc ggggagatgg tcgggacgat ctggccgatc
     5461 ggccccgcca cctcgaagag gccggcgagc ccggcgaggc cgccgccgag caggaggctc
     5521 gtccagatca tcgccttctg cgagaagccg gcgaagcgtg ccgccgccgg cgcctggccg
     5581 atcaccgtga cctggaagcc gaagagcgtg cgggccagga ggaaccagac gacgaggacg
     5641 acgaggacgg tgatgggcat gcccaggttc agccgggtcc cggcgaggat gcccggcagc
     5701 agggcactct ccgagaacat gcgggattcg ggaaagccga agccctcggg actcttcaaa
     5761 ggcccataga tcaaagcgct caggagcagg aggcccacat aggtgagcat caggctcacc
     5821 aggatctcgt tgacgtcgaa gcgcgccttg aggaaggccg ggatcgccgc ccaggccatg
     5881 ccgccggcga ccccggcgat gcccatgagc ggcaggatcc accagccctc ctggttccag
     5941 aagagcagcg ccacgctgcc gccggcgagc gcacccagcg tgagctgccc ctccgcaccg
     6001 atgttccaga cgcccgcgcg aaagccgacg gcgaggccgg tggcgatgag gatgagcggg
     6061 gtcgccttga ccccgagctc ggcgaggcca tagcggctgg tcaacggatc gacgaagaag
     6121 gccctgagcg cccggatcgg gtcgtagccc atggcggcga agagcacggc cccggtcagc
     6181 acggtcagcg ccacggcgag aagcggcgtc agccagcgca tcttgcggct gatcgcggcc
     6241 cggggctcga gcctaaccat gcgtcgtctc cggctggccg tcgccctggg gcacctcgtc
     6301 cggcaggtcg tgcagcccac ccatcaggag gccgatctgc tcgaccgtgg cctcggcggt
     6361 gaccagcggc ctggacagcc ggccgccgtt gagcaccgcc atgcgatcgg tgagcgccat
     6421 cagctcgtcc aggtcctggc tgaccacgag gacggcgacg ccgtggccgg cgaggtcgat
     6481 gagcgcctgg tggatggcgg cagccgcccc ggcgtcgacg ccccaggtcg gctgggcggc
     6541 gatcagcacg ttgggcgcct ggaggatctc gcggccgacc acgaatttct gcaggttgcc
     6601 gccggagagc gccgaggcgc gggcagttgg cccggtcgcg cgcacgctga agcggtcgat
     6661 gatctcctgg gcgaaggcgc tcgcccgggc atggcggatg accccggacg agagcaggcc
     6721 gcgccggtcg aaggccgaga gcagcgcgtt gccggcgagc gtgagatccg gcaccgccgc
     6781 atggccgttg cgctcctcgg ggatgtaggc gaggccgtgc atgcggcgcg cccggggccc
     6841 gagccgaccg accttctgcc cgtcgatgac gacgaaaccg ggctcggtca gccgctcgcc
     6901 ggaaagcgcc gccagaagct cgctctggcc attgcccgcg accccggcaa tgccgaagat
     6961 ctcgccgacc tggacgtcga agctgatctc gcgcaaggcc gtgccgaaga cctcggcggt
     7021 cggcaggtcg agggcgttga ccgagaagcg gggagcccgc ttcaggcgcc tggtcggctt
     7081 ctcgggtgcg gcgaaggagc tgcccaccat gagctccgcc atgctcctcg ccgtctcggt
     7141 ccgggggtcg caatgcgcca ccaccttgcc gccgcgcatg atggtggcgg cgtcgcagag
     7201 ggcctcgatc tcgtgcagct tgtggctgat atagaggatc gacacaccct ccttggcgag
     7261 ctggcggagc gtcgagaaga gccgctcgac ctcctgcggg gtcaggaccg aggtcggctc
     7321 gtccatgatc aggagccgcg ggctctgcag caggcagcgg acgatctcga tccgctgccg
     7381 ctcgccgacg gagagttcgt gcacctcgcg gtgcgggtcg agcggcaggc cataggcctg
     7441 cgagactttg gtgatgcgct cggccagggt cctgcggtcg agcttcgcgt cgaggccgag
     7501 ggcgacgttc tcgagcaccg tcagggcatc gaacagcgag aaatgctgga acaccatgcc
     7561 gatgccgagc gtccgcgcgg cgtggggcga cggaatcgtg accggccggc cttcccaggc
     7621 gatctcgccg gcatcggggg cgagcacgcc atagatgatc ttgaccaggg tggacttgcc
     7681 ggcgccgttc tcccctagca gcgcgtggat ttcgccctgg cccaccgtga gcgccacgtc
     7741 gtcattggcg agcgcgcccg gataggcctt cttgatgccg gagaggacga gccgggccac
     7801 ggtcatcgag cgccgctcat cgaacgaggg acatcaggcg acgctcctga tttgccggcc
     7861 ccgggtcagg gcctccatga cgacgagcag ttgcgccgtc accgaggcgg cgatcacggc
     7921 gggcgccttg ccggtgatgg cggcgaggcc gatcgggcag accagccgct cgaccgagag
     7981 cccgacggcg ccgagctgcc gctcgaagcg ccgccgcttg gtggccgagc cgatcagccc
     8041 gagccacgcg aagctgcccc gccgcagcac cgcctcgaca atggcgagat cgagcggatg
     8101 gctgtgcgtc atgaccaaga cgaggtcgcc cggcgccgtc gcgaacccgg ccggatcggc
     8161 aatttcacga gtttcgacca gccccgacgc atccgccggc aactggcccg ggcggctgtc
     8221 cagccagagg acacggcagg gcaagggggc gagggccgcg accacggccc ggccgacatg
     8281 gccggcgccg aagagaacga cgcgcggcag gcccgcctgc catgctccca gccgttcggc
     8341 caacatcgtc cggtcgcctg gcgccagatg ccgaaggcgc agcgtcacgt gcccgccgca
     8401 gcactgtgcc aaggccgggc cgagcggcag gtcgagacgc tccggcactg gctcgcccgc
     8461 cagcgcctcg cgggccctga ggatcgcccg atgctcgagc tcgccgccgc cgatcgtgcc
     8521 ggcgatgctg tcgcccgtga ccagcatcag ggcccctgcc tcgcgcggcg tggagcctag
     8581 cgcctcgacg atctcgacga gaatcgccgg ctccgactgg tcgatggtag caagggcggc
     8641 gacaagggct tcggtcatgc gaggcccgcc agccggtcga tggccatcag caccttctcc
     8701 ggcgtcgccg gcgggtcgag tcgggccggg tgcttgtggc ctgccgtcgc ggccacggcg
     8761 tcggagagcg cctgccagac cgagatggcg agcatcaggg gcggctcgcc cacggccttg
     8821 gagcgatgga tcgtggcctt ggggttcacg ttctcgagca gacggatgtt gaagatctcc
     8881 ggccggtcgg cgcaggtcgg gatcttgtag gtcgagggcg cgtgggtgcg cagccggccc
     8941 tccttgtccc agaccagctc ctcggtggtg agccagccca tgccctggat gaagccgccc
     9001 tcgatctggc ccatgtcgat cgccgggttg agcgagcggc cgacgtcgtg gacgatgtcg
     9061 acccggtcga cccggtactc gccggtgagc gtgtcgatcg tgacctcgct gcaggcggcg
     9121 ccataggcga agtagaagaa gggcttgccc cggccggtgg cgcggtccca gtggatctcc
     9181 ggggtcttgt agaagccggt agcggagagc tggacgcggg ccatataggc gtcgtggacg
     9241 agccggtcga aggtgatctc gtcgcccgcc accctgaccc ggcccggcag ccagtccacg
     9301 gcgtcggccg gcatctggtg ctgctcggcg gcgaaggcga ccagccgcgc cttcagctcg
     9361 cgcgccgcgg cctgggccgc catgccgttg aggtcggagc ccgaggacgc ggccgtggcc
     9421 gaggtgttcg gcaccttgcc ggtggtggtg gcggtgatct tgatgcgttc cagcggcagc
     9481 gccagctcct ccgccaccac ctgcgccacc ttgatgaaga gcccctgccc catctcggtg
     9541 ccgccatggt tcaggtggac cgagccgtcc ttgtagatgt ggatgagcgc accggcctgg
     9601 ttgaaggcgg tgttggtgaa ggaaatgccg aacttcaccg gcgtcagcgc caggcccttc
     9661 ttctgccagc ggttgtcggt gttgaagcgg ccgatggcgg cccgccgcgc cccgtagccg
     9721 gcatccttct cgcattcggc caccactcgc tcgatgacat tgtcctcgat ggtcatccag
     9781 tagggcgtga ccgcgcgctc gttcggcccg tagaaattgc gcttcctgac ctcgagcgcg
     9841 tcgagaccgg tgcggatcgc gacctcctcg atcactcgct cggccgccac catgccctgc
     9901 ggcccgccga agccgcggaa tgcggtggcg gagaccatgt tggtgcggca gggatggcag
     9961 acgagcgcca cgtcggggta gaagtagcag ttgtcggcgt ggaagagggc gcggtcgttg
    10021 atcggcccgg agaggtcggc cgagtagccg cagcgcgagg cgaaggtcat gtcgacggcg
    10081 cttatgcgcc catccggctc gcagccgacg gcgtagtcga ccaggaactc gtggcgcttg
    10141 ccggtcatga tcatgtcgtc gtcgcggtcc agccggcact tggccgggcg gccggtccgg
    10201 cgcgccgcga gggctgctat gcaggcgaag agattgccct gcgtctcctt gccgccgaag
    10261 ccgccgccca tccggcggac ctcgacggtg acgctgtgcg acggcacgcc cagcacatgc
    10321 gccaccatgt gctgcacctc gctcgggtgc tgggtggagc tgtagaccat catctcgccg
    10381 tcctcgccgg ggagagccag ggcgacgtgg ccctcgaggt agaaatggtc ctggccgccg
    10441 atcgcgatcc ggccatcgag gcgacacggg ctcgccgcca gcgcccgcac tgcatcgccg
    10501 cgctgcatcc ggcccggcgg ccagacatgg tcctccctgg cgcgggcctc gtccacgtcg
    10561 agcagcggcg gcaggtcctc gtactcgatg ctggccagcg ccgccgcggc gcgcgccgcg
    10621 tgcaccgtcg gcgccgccac ggcgaagagc gcgtggcccc agtactggac gatgccggtg
    10681 gcgaagaccg gctcgtcgtc gcggccgacc gggctgatgt cgttcacgcc cggaatgtcg
    10741 tcattggtca gaacggccac gacgcccggc gccgcgcgga cggcggcgag atcgagcctc
    10801 ttcacacggg catgcgcacg ctccgcccag gcgatataaa tctcgaggca gcgggcgggc
    10861 tccggcatgt cgtcgacata gaccgcctcg ccggtgacgt ggcgaagggc gctgtcatgc
    10921 gccaaggcgc ggccgatcgg gctcgcctca gacatgcacg ggctcgcgcc tgccggcgag
    10981 gcggagcgac ggcgacgtgg cgtcctccgc ttccaggtac gccttgagaa gcaaattttg
    11041 agccaccttc agccggtagg cggccgaggc ccgcatgtcg tcgatcggct ggaaatccgc
    11101 cgccagggcc gccatggcgc gccggaatgt cgcctcggcg aagggctggc cgatcagcgc
    11161 cgcctcggcc cggagggcgc ggcgcggggt cgccgccatg ccgccatagg cgatcctggc
    11221 ctcgacgatg cgatcgccgt cgcggcggag ctggatgccg gcgcagacgg ccgagatgtc
    11281 ctggtcgaag cgcttgctga tcttgtagca gcgcagctcc gtccccgcct cgggcttcgg
    11341 cacgctgact gcctcgacga tctcgcccgg ccgccggtcc tgcctgccat aggcgaggaa
    11401 gaactcctcg agcggcagcg agcggcggcc gtcgcgatgc gccaggtgca gcgtcgcccc
    11461 ggcggcgatc agccagggcg ggctgtcgcc gatcggcgag ccgttggcga tgttgccgcc
    11521 gatcgtgccg gcatggcgga tctgctcggc gccgagccgc cggaccagct cgccgacatc
    11581 cggccagagc ccggccagac gctcgtgcgc cgcagcatag gtgaccccgg cgccgatgtg
    11641 gatggcgtcc tcggtttcct cgattcgggc cagcccggcg acccggccga gccagatgac
    11701 cggatcgagc cggcgctgct gcttggtgac ccacaagccg acatcggtgg caccggcgag
    11761 gatcgtcgcc tgcgggtgat cggccgcgag gtcgagcagg gcgtcgagct ccgtcggggc
    11821 gtagatcatc cgcccgccct cgccgatggc cagggtctcg ccgtcgcgcc aggaatcgag
    11881 gcgggccagc acctcggcct cgcggtgagc gagatgcgcc accgcgcccg gcgtctcgcg
    11941 catccgccgg gcggcctcga tgatcggccc gtagccggtg caacggcaga gattgccggc
    12001 gagcgcgtcg tcgatccggc cgcgcgacag cgccgtgtca ccattctggt ggagggcgaa
    12061 gagggtcatg acgaagccgg gcgtgcagaa gccgcattgc gagccatggc tgtcgaccat
    12121 cgccgcctgg accgggtgga gcgtgccgtc cggcgccttc aggtcctcca cggtcaggag
    12181 ctggcagccg tccagcgtcg ccatgaagcg gatgcaggca ttgatcgcgc ggtagcgcag
    12241 ccgcccatcc tcgggctcgg cgatcaccac gctgcaggcg ccgcaatcgc cctcggcgca
    12301 gccctccttg gtgccccgcc tgtaggccac ccggcgcagc cactcgagca ccgtcagggt
    12361 aggatcgatc gcttcgagct cgaccggctc gtggcccagc aggaagcgca gccgctgccg
    12421 catcaactgc cccgataggt cgagtaggcg aagggcgaga cgacgagcgg cacatggtag
    12481 tccgcacccg gatcctcgat gccgaagcgg atcggcacct cgtcgaggaa gggccggttc
    12541 ggcagatcga cacccagcag cttgaaatag tggccgacct gaaagatcag ctcgaacgtg
    12601 ccccggccca tggacgcacc ctcgagcatc ggccggtcga gccggccctg ctcgttggtc
    12661 cgggcctcgg cgagcttgac ccggtcggcg ccatccaccc gatagagcgc gagctgcatg
    12721 ccgtgcccgg gtcgccccga catcgtgtcc agaacatgcg tggtgagctt gcccatcgcc
    12781 cgtccctgcc gtttccgttg aacgatcaat ggttaatcgc agagcgggcc gctcacaatg
    12841 gcctaaccgg catcattcgg ccgagattgc cacccgcgtc attggatcgg cataaaggca
    12901 ggtggtcatg ccgcccgcgt actcgtcgac cagttgtccg tcgagcgagc cgcgcgcctc
    12961 gatctgcgcc gcgagccggg ccgtgagcgg gctctcggga aagcgggcac ggtaggcctc
    13021 gaaccaggcc atgtagtggc gatcgtggcc gcgccagtcg agaaagccca gatcctgcgg
    13081 tgccttggtg tagggggcga ggacggccgg atcgtcgatc ccggcgacga ggaaagccac
    13141 catctggtgc aggtcgaggc cggcctcgct ggtcagcgcg tagaggtcgt agccctgcac
    13201 ggcggcgatc tcggccatgg cgacgagcga ggcgatggca tggcgctggt agaagagggc
    13261 gtcgcggccg cgatcggtct cgagcggcag gctgccatcc tcgcgcatct gctcgagcgc
    13321 cagccggtag cggttcacac cctcgaagaa gagcgcgttg tcgccggtga aggcgcccca
    13381 ggccatggtg acgctgtcgc gcaggtagcg gtgattgttg cggctgctgg ccacctcggg
    13441 gttggtcacg cggtcgggcc cgcgccgccg gaccagccgg tcgagccagg cgcgaatggt
    13501 ggccacctcg tcgggcgcca gcgccggatc gtcgtgcacc atggcgaggc ctgtcacgat
    13561 cggcagcagg gtgcggtcga gattgtagaa atggttgacc gtgaccggct cggtcatgcg
    13621 cgagagcgca tcggcctcgg cgaaccgcag caggttggaa atgatgccat cgcgggcgtc
    13681 cggatcgccc gtcgcgagat agctcgccgc tgcatgcatc atggtgagtg cgagggtctg
    13741 cgccggctgc gcgccaccgc cgacattgcg ctgcacgccg tggcgcggcc agctctggcg
    13801 aggcgggccg cagagcgcct cgcgctcggc ttcgggcagc gcctcgagca ccgcccggcg
    13861 tgccgcgaca tcgaccagca cgaggcccgg ccgggtgacc acgaactcgc tcggcgccaa
    13921 ggcgctggcc gccacgggtg cgtcggccga gagcgccggc agcgagaggc agaggacgag
    13981 cgtgaaggtg aggccggcgg ccacgcggct tggcaagctt tgggctcccg tctagttgtc
    14041 gggctcatgt gtcgggaggt ctaacggata gacagccgga caaccagtgg aggcaattga
    14101 tgaagagccc gtttgacctc ggcggcaagg tcgccctcgt gaccggtggc aatggcggca
    14161 tcggcctcgg catggcgcgg ggcctcggcc gtgccggggc ggcggtcgcg gtggccggtc
    14221 gcaaccaggc gaagacggcg gccgcggcgg ccgagctgac gagcgagggc gtccgggcga
    14281 tagccgtggt gggcgaggtc gccgatcctg ccgcctgcgt cgccatggtc gaggaggtcg
    14341 aggccgagct cggccggctg gacatcgccg tctgcaatgc cgggatcaac atcaggaagc
    14401 gccccgaggc gtacgagttc gccgaatggc gcgaggtgct ggcgatcaac ctggacggcg
    14461 tgttcaacac cgctcacgcc gcctatcccg ccctctgccg gcagggcggc ggcaagatca
    14521 tcaccacggg ctcgatgatg tcgatcttcg gcatcccggt ggttgcggcc tacgcggcga
    14581 gcaagggcgg cgtgatgcag ctcagccggg cgctggccac ggcctgggcc agggacaaca
    14641 tccaggtcaa ctgcatcctg ccgggctgga tcgacaccga cctcacgcgc cggggccggg
    14701 ccgagatcga ggggctgaac gagaaggtgc tgacccgcac gccggccggc cgctggggca
    14761 cgccggagga catggccggg ctcaccgtgt tcctcgccag cccggcctcg gacttcatca
    14821 ccggcaccgc cattccagcc gacggcggct actcgatcca gggctgaggg atcagggccc
    14881 gatcgggccc gatggccgct cgaaccgcag gagccgcgtc ccgccggcgt tgagcggctg
    14941 ctcctcgacc tgaagcaggc cgagccccga cagcgcatgg cacggcttca tgccgcgcga
    15001 ccaggagaag agcggcagcc cgccggcgaa agtcagcggc tcgatggtga gctcgacggc
    15061 gtcgatcagg cccgcggcgg cgaaccagtc atgcaccgcc acgccgccca ggatgccgca
    15121 gctcacggcc ggatgaactg ccgtcgccgc tgccagcatc gcgtccaggg agacgcgccc
    15181 ggggtcggcc cagagatgca ggggatggcg ccattccggc ccggccaggg cggccgtgaa
    15241 gacgacgcgt cggcgattcg gccgccaggc cagctcgtgc gtgcgccgcc ccatgaaagt
    15301 ccagtcgagg ccggcgacgc gatggcgaaa gagccgttgc tcctcgcgcg acgcccagac
    15361 gcccggcggc gcaccgccct cgggcgcgat gtagccgtcg cggctggtga cgacgatcag
    15421 cgtgaagcgc ggccgtgacg gcacaaccat gaccggctgt tccccggcca gggacgcatc
    15481 gactcccgtt ccgtgtccga gcgggccctt cagcccagga ggagcgagaa cgaagcgatg
    15541 atgatcgtcc agagcgccag gctggcagcg acgatgacga gctgcggtgg cgtgccgttg
    15601 gctgaactga ccggaacgcg ccgcgcaccc ctacgggtgg agacggtgcg catgtgacct
    15661 agtggaccct gtggtgggag catgatcttg ccgatgcctc atccttgcga cctagcagcg
    15721 cagcagcgcc acttttcttt tcttatggcg aaattgtagc agaagcaggc gagagagaaa
    15781 agcgatattc cacctataag agagaaagtc cccacgctac tggaaacctc aggttgaggc
    15841 cagccgcagt gggccggcct cacccgttcg tgtcagtcgc gcgccagccg ccaggcgatc
    15901 ggaaaaaaag cggtcgcaag caggagcttc gcggcctcgg ccggcaggaa aggcaagaca
    15961 cccgcggcaa aggcctttgc cggcccgatc aacaaggcaa gccacgttgc accgagcccg
    16021 aaaatcaaga gggtagcggc gagaaagacg aagaaggccg atagatgcgt gcggccgagc
    16081 ccctcgtccg caagataacc gacgacgacg gctgcgacaa aaaagcccac cagatagccg
    16141 ccggtggggc cggccagata ggcgagccca gcaccggtcg cgaagaccgg caggcccatc
    16201 gccccttgcg ccagataggt caggagcgtg atgccggcga gcctggcacc gtacgcggca
    16261 ccgatgccga ggacgacgaa ggtctgcatc gtcatcggca ccggccagaa cggcacctgg
    16321 accctggccg agacggcgag caggagcgtg ccggcgagca ccagcacgat cctggcggga
    16381 agactttggg ccgagctcgg ccagagggcg gtggtcagcg tcgggttcat gctctgctgc
    16441 tccgaaggtg ggacgaatgg ctcatgccag atggctttgc atcttgtcga gaatggcctg
    16501 ccggtcgtgg cgggcggcga gggcgccggc catgtcgacg gcgacgaacc tgacgggtgt
    16561 gtgcggctgg agctggccga tcaggtccat gtcggccgag atcaccgtgc cgaccatcat
    16621 gtagccgcca ccggaaaccg cgtcgcgatg cagcacgatc ggctcggtgc cgcccggcac
    16681 ctggatcgag ccgtagggat agcaggcgtc gacgatgttg gagggatcgg agcccgcgcc
    16741 gaacggctgc tcgtgcggca tgaactcgag cggccggccg ccgcggaagc gatagccgat
    16801 gcgatccgcc tcgggcgcca ccttccacgt gtcggcgagg aactgccggc cggcggcctc
    16861 ggtgagccgg tgccagtaga ggccgggcag gacgcgcagg ctcaccggcg agccgcccgg
    16921 gccgcgccgc agcgctgccg ggatctcccg ccccgccgcc gccggcctgc cccggccgac
    16981 cggcaggaca tcgcctgccc ggagggcgcg gccctcatgg ccgcccaggg cacccagcgc
    17041 ataggtcgag cggctgccca gcaccaccgg cacgtcgatc ccgccggcga cggcgacata
    17101 ggcccgggcc cccgccttca ggtagtcgaa ggccagggtc tgcccggcct tcacggcgaa
    17161 gctcgtccag gtcggctgca ccacgccgtc gaccctgggc ggcagctcgg cgccggtgac
    17221 cgccaccatg gcgtcggccc gaaagaccag ctcggggcca aggaacacga cctcgagcgc
    17281 cgcggcacca tcgtcattgc cgaccagccg gttggcggcc cgcagcgcca ggcggtccat
    17341 cgccccggac agcggaatgc cgagatggta gtagcccggc cggccgagat cctggacggt
    17401 ggtggcgagg ccgggcgcct cgacctcaat ggccatcgag cacctcctcg agcttccggt
    17461 tgtagccgtc gatatcggcg ttgaacgcgt cgagcgagaa ggtgacattt ctcaccttcg
    17521 gcgcatagcg gccggccgcc acctcctcga ccacgtggtc gtaataggcc cggtcgaccg
    17581 acttgaactt gacgatgtcg cccggccgga agaggcacat ggtgtcgtcg ctgatgtagt
    17641 cgatctcgct cttcggatcg tagatcggca ccggggtgat gccgaacatc tggtagccgc
    17701 cggccccgcg caccgagtag atcgcggtga agcagccgcc atagccgacc gtcagcttcg
    17761 gcgtgtcggt gcgcggccgg aggtatttcg gcacctcgag ctgccgcgcc cgctcgacca
    17821 tctggaagag gaacggcagg ccggcgacga acccgaccat ggagacgaac cagggagccc
    17881 ccgaatgggc ggcgatgaac tcctcgacgc tggccttgtt gttgatccgg gcggcgtagt
    17941 cgagatcggt ggcgctcggg tcctggtggc ggtcgcgaaa ccgcatcagc gtctcgtgcg
    18001 tccaggggtc gttgtagtag accgggaact cgaagatccg cgtctcgatc accgagggcg
    18061 cctgcgccgc cgcggcctcg agccgcttca gctcggccag catgtcgtcc ggcttgatcc
    18121 ggtcgggatc gaacttgatc tggaaggagg cgttggccgg gcagatctcg gtgaccccgt
    18181 cgatccggct ctcgcgcacc gcgttggtga tcgagagcgc cttgaagaag gcctccagcg
    18241 acatgctctc gtcgacctcg acgaagatgt gctcgtcgcc gccgaaggag tagcggttgg
    18301 tcatgccgtg ggcacctccc ctgtacggtc cgtcgccggc gcgagccgcg ccgcgttctc
    18361 ctcgagccag cgctcgagcc aggtcgtgtg gtagcgggcg gcccgcacat ccggatcggc
    18421 cgcaagcgcc aggtgcaggg cgcgcgtggt cgccacgccg ccgatctcga gctcgcccag
    18481 ggcccgcgcc agacgcgcca gcgcgtggct gcggtcctcg tcccagacga tgagcttgcc
    18541 gagcagcgaa tcgtagaagg gcggcaccgt gtagccggca tagagcaggc tgtcgaagcg
    18601 cacccccggc ccgcccggca ggctgagccg gtcgatcgtc ccgggaccgg gcgcgaagtc
    18661 ccgggccggg tcctcggcgt tgagccgaac ctcgatcgca tggccatgca accggatctg
    18721 atcctgggtg tggcgcagct tctggccgag gcacacgcgg agctgctcgc gcaccagatc
    18781 gatgccggtc accatctcgg tcaccgggtg ctcgacctgg atgcgggtgt tcatctcgat
    18841 gaagtagaag tcgtggctct ggtcgtcata gagatattcg agcgtgccgg cgccgcggta
    18901 gttgaccgcc cgggcgaggg ccacggccga ggcgcagagc ttcctccgca cggccgccga
    18961 cagccccgcc gacggcgcct cctcccagac cttctgccgg cgccgctgca gcgagcattc
    19021 gcgctcgaag agatgcacga cgtgctggcc gtcgccgagg atctgaacct cgacgtggcg
    19081 cgcccgctcg accaccttct ccagatagag cccgccatcg ccgaaagccg ccttggcctc
    19141 cgcgctggcc tgcggcagca gccgctcgaa ctcggcctcg tcgcgcacga tgcggatgcc
    19201 gcggccaccg ccgccggccg ccgccttgat catcagggga aagcccgttc gggcgaccac
    19261 cggccgcgcc tcgtggagcg tgcggacatg gccctcgctg ccgggcaccg tgggcacgcc
    19321 cgcggcggcc gccgcctggc gggccgccac cttgtcgccc atcagccgga tggcgtcgcc
    19381 ggtggggccg atgaaggcga gcccggcggc ctccacggcg tcggcgaaat cggcgttctc
    19441 ggcgaggaag ccatagcccg gatggaccgc tgtggcaccg gttgacttcg ccgcctcgag
    19501 cagggcctcg atcttgaggt aggacttgct cgcctgcggc gggccgatgt cgaccgcctc
    19561 gtcggcgagg cgcaccgcca gcgagtcggc gtccgccctg ctgtgcgcct ggatcgtgac
    19621 gaagccgagc tccttggccg cccggatgat ccggaccgcg atctcgcccc gattggcgac
    19681 gaggagccgg acctcgcgca agacgccggc tcagccctcg aggtcggcga tggcctggcc
    19741 ggccatgacc gcgtcctcgt cgtcgatgag gaaagccccg agggtgccgg cggcgtccgc
    19801 cttcacctcg tggaaggact tcatcacctc gaccaggccg accacatcgc cgacctcgac
    19861 cgtgtcaccc tcgctcttga acggcggctg gtcgggagcc ggccggcggt agaacgtgcc
    19921 gggcaacggc gacaggatct gcttggacgc catgctccac ctcttctgct cgctgcggcg
    19981 gaggttgggg aaggcggagc ggcttgtaaa gcgggaagag acggcctcag gcggtccgtg
    20041 acttccggta ggaatccttg agcgcgatcc gctcgccggc gaaggtgaag agatcgcagc
    20101 catccacctc caccgtggca ccctgccggt cggtgccgac gaaccgccag gacgagaccc
    20161 cgtggtcgcc cgccacccga tgctcgccct gcgtccaggc ggccttcggg aactgctcga
    20221 agatcgccgc ataggccgcc cgcacggcgt cgccacccgc gtgccgtcgg ccgtcggcag
    20281 ccggcccggc ggaagcatgg aaggcgcagt cctcggccat gcaggccatg agcgcatcga
    20341 cgtcgtgcgc gttccaggcc gcggcgaacc gctcgaggca ggcgagccgg gcggcctgca
    20401 gcgcatcgcc cagcacctcg acctcgagga acgccatggc ctcgctggcg tcgttgacga
    20461 cattgtgctc gacgccggtg cggcgcgcat agggcacgtg ccgggtgagc gacgccttgc
    20521 ggctcgtgcc acccggctcc tcgagcagga gcgtgccgtc gaccagggga acgaccacgt
    20581 agtcgtggcc gtggacatgc cagccggtct cggcacccgg cgcgaagcgc cactcggtaa
    20641 ctcggaaacg accgtcgtcg agatgcgtga cgggctcggc tgggggacgg gtcatggggc
    20701 gtgtactccg gcagcaccgc ccgggaccgg ccccgggacc ggcgttcatg aggacctggt
    20761 ggagccgacc gggatcgaac cggcgacctc tagagtgcga ttctagcgct ctcccagctg
    20821 agctacggcc ccacgggtcg cggctctatc ggatcgaccg gggtcggctg tcaagcgtct
    20881 tggcacccgc ccgcccgccg cggcgtccca tcgacaccct tgccagccga ttggccgcac
    20941 cctacttgcg ttgcgccagc cctgcccgcg gccagcacca gaacgaagat gatggaggcc
    21001 cgatgatcgc cgtctacaac ctcatcaaca ccgtgatctc gatctatatc tggctgctga
    21061 tcgcgatggt cgtgctgaac ctcctcgtct cgttcaacat ggtcaacacg ggcaaccgct
    21121 tcatctacac gatcggcgac ttcctgaacc gcatcaccga gcccctgctg cggccgatcc
    21181 gcaatatcct gcccaatctc ggcggcatcg acatctcgcc gatggtcctg atcctcgccc
    21241 tctacttcat ccgcgacctc gccttccagt tcctcgtgct ctgaatgacg ccaccctggc
    21301 ggctcgagcc ggaccgggcg atcctcgaga tccgggcggt gccgggggcg cggcacgagc
    21361 gcgtcgacgg catcgccatg gacgcccggg accgcccgca tctggtcatt cgactccggg
    21421 cacccgccat cgagggcaag gccaacgcag cgctcctggc ctttctcgcc aaacgcctcg
    21481 actgcccgcg gtccaggctc gagctcgtgg ccggcgccac cggccggctg aagcgtgtcc
    21541 tctggaagga cccgccgacc gacgcggcaa ggcggctcgc cgccctgcac ccggcgcccg
    21601 gcgacggctg agcgcgaggt ttgcccctgg ctgcgggatg cgctaccagc cctccacccg
    21661 ggccggtccc agcgccctgg catttccacg caacgacgag gcagatgaag atgcgcgctt
    21721 ccggcagggc ggccgatgag cttcgtgagg tcgttctcga gccgggtgcc aacccgcagg
    21781 ccgagggctc ctgcctcgtc cgtttcggcc acacgcacgt cctctgctgt gccacggtcg
    21841 aggagcgggt gccgcccttc ctgcgcaaca ggggcacggg ctgggtcacc gccgagtacg
    21901 gcatgctgcc gcgctcgacc cacagccgct cgccgcgcga ggcggcccgc ggcaagcagg
    21961 gcggccgcac cctcgagatc cagcgcctga tcggccgtgc cttgcgcgcg gtcaccgacc
    22021 tcgaaggcat gggcgagcga cagatcatcg tcgattgcga cgtgctgcgc gccgatggcg
    22081 gcacccgcac ggcctcgatc accggcgcct acgtggctct ccaccaggcg ctcgccaagc
    22141 tggtgcgcga cggcgccctg ccggccctgc cgctgaccga cgccgtcgcc gccgtctcct
    22201 gcggcatcgt cggcggcgag gccctgctcg acctcgacta tcccgaggac agcgccgccg
    22261 aggccgacgc caatttcgtc ctggccggca agggcggcat cgtcgagatc cagttcaccg
    22321 ccgagaagga gcccctgccg cgacaggacc tcgcccgcct gatgaccctg gccgagggcg
    22381 gcatcgagca gctcctggcg ctgcagcgtg ccgccgtcca cgccggctga cgggcccatg
    22441 caccggcgct tcaccggcga caccctcgtc ctcgcctcgc acaacccggg caagctccag
    22501 gagctgcagg agctcctggc cgaccacggc gtaaaggtcc gctccgccgg cgagctcggc
    22561 ctgatcgagc ccgaggagac cgagcccgac ttccagggca atgcggccct caaggcgcac
    22621 gccgcggcca gggccacggg cctgccggcc ctcgccgacg acagcggcct cgaggtcatg
    22681 ggcctggagg gccggccggg cgtgcattcc gcccgctggg ccggacccgg caaggacttc
    22741 gccgccgcca tgacccgcgt caccagcgag ctcgccgagc gcttcggcga ctggcagcgt
    22801 gtcgacaagc gcgccgcctt cgtcagcgtg ctctgcctcg cctggcccga cggccacgcc
    22861 gaggtcttcg agggccgggt ctcgggcgag ctcctcccgg agccgcgtgg tgcgggcggc
    22921 ttcggctacg atccgatctt cgtccccgag ggcgagaagc gaaccttcgc cgagctctcc
    22981 cgcaccgaga aatcggcgct cagccatcgc ggcatcgcca tgcgagccct cctcgacgcc
    23041 gtcttccgct gatcccccga gccttgcgca tcacggcacg atcaaccata tccacgcccg
    23101 aggggccgta gctcaactgg gagagcgcct gctttgcaag caggaggtcg tcggttcgat
    23161 cccgatcggc tccaccaacc gaatcgacat agcgcccgga ccagggcgac gccgccgccg
    23221 cagatcgcta cgcgagcccg cgcgagcccg gcttgatcgc ggggatggcg gcgaggtcgg
    23281 ccggcacctc gtcaggcgcg taggtcggaa agtcgacggt ggcgagggcg gtgagcttca
    23341 tgccgatatc ggccttgccg ttgctgcggt cgatcagcgc cgcggcaccc acaaccacgg
    23401 ccccggtggc gcggaccgcg tcggcgcatt cccgcaagga gaggccggtg gtcaccacgt
    23461 cctcggcgat cagcaccctg gcacccggct cgagggcgaa gccgcggcgg agcgtgaagc
    23521 ggccctcgac ccgctcggtg aagagcgaga gacagcccag cgcccgggcc agctcgtagc
    23581 cgaccaggat gccgcccatg gcgggcgcca ccacggcgtc gatggtcgtt gcggcctgcg
    23641 cctcgcggac tcttgccgcc agcgccgtgc agagccgctc ggctcgggcc gggtccatca
    23701 gcacgcgggc gcattgcagg tagaaggggc tgcgccgccc gctggtcagg aggaagtggc
    23761 cctcgagcaa ggcaccggcg gcgcggaact cgtcgagcac ggcgtccgtg gtcaggctca
    23821 cgagcggctc cgctcgaccg tgctgacgat ggggttggcg cggagcgctg cctggatgcg
    23881 ctggagctgg tcgacgtccc tgacctcgac atcgagcagc atctcgaaga ggtcgggcga
    23941 gcgacggccg accttgacgt cgacgatgtt gccctcttgc ttgccgatga tcgtgctgat
    24001 ggtgcccaag gcacccggct ggttggtcac catgacggag atgcgggtcg aggcgagcgg
    24061 cggctcgccc tgcccgctcc agccgatatc gacgaagcgg tgctcgctct cgcccatccg
    24121 ccccacggtg gcgcagtcgc ggcaatggat ggagatcggc cggccggtgc gcacgatgcc
    24181 gacgatgggg tcgccgggga tcggccggca gcagccggcg aactgatagg cgatgccgct
    24241 cggcagcccg gcgatgacgc ccgagcgacc ctcgtcgctc tcggccggcg ggcgcttgaa
    24301 gaggacgatg ttgtcgaggt cctcaccccg ctcgcgttcc gcaaccctgc cgtcccgggc
    24361 cggcagccgg agcgccgggg cggggatggc ctgcagcacc agccgggcgg cgagctggcc
    24421 ctcgcccacc tggaccagca tgtcctcgag gctggcatgg ccgagctcgg tcgccgcctg
    24481 ggcgagctgc ttctcggtcg gctgcatcgc ctcggccctg gccgtccggg ccagcatctc
    24541 gcggccgcgg gtgacgtagt cgtcgcgctg gcgggccttg agcgcccggc ggatccgggc
    24601 cctggccttg ccggtgacga cgtagcgctc ccaggccgcc gtcggccagg cgttgcggct
    24661 ggtgatgatc tcgacctgat cgccgttctc gagggtgtgg cgaagctgga ccatgc
//
LOCUS       DAOTFI010000035        24048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2459_length_24048_cov_6.874380, whole genome shotgun sequence.
ACCESSION   DAOTFI010000035 DAOTFI010000000
VERSION     DAOTFI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 24048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24048
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2459_length_24048_cov_6.874380"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="P5558_05220"
     CDS             <1..492
                     /locus_tag="P5558_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY23740.1"
                     /translation="LVESVWTPGWDPSKAQQQMEQILTRTNNGVDAVLSANDGMAGGV
                     IAALQAQQMAGEIPVTGLDGTLNGLQLILLGRQSMTVWRSLKDQSGKAAAITAALLKG
                     EKPADSMFEGATMNNGATEIPWAKVTPIVITLDNMELEIEDGAVTREALCRGMPAVGP
                     CKP"
     gene            505..1299
                     /locus_tag="P5558_05225"
     CDS             505..1299
                     /locus_tag="P5558_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY23741.1"
                     /translation="MTSGRADGAGSAVLQLEGISKRFGAVEALSEVDFEVRAAEVVAL
                     AGDNGAGKSTLVKVIAGTHAPDGGVIRFDGVEVSIRSPRHAASLGIETVYQDLALCDN
                     LDVVANMYLGRETVGRPRFLQRLSELDMERHALDVLRRLNVTTIRSVRAAVATLSGGQ
                     RQSVAISRAVMWNSKIVLLDEPTAALGVAQTHEVLELIKRLRAQGLGVVLISHNLHDM
                     FEVADRIVVLRLGRNAAAFRCRETTPEEVVAAITGAREAAAREAVC"
     gene            1293..2540
                     /locus_tag="P5558_05230"
     CDS             1293..2540
                     /locus_tag="P5558_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRY23742.1"
                     /translation="MLKQAQDARDRMSGSRAGGRARTGASLTTLARGLRLRSDSSELG
                     VLPIVLGLAIIWTFFQLQDAHFLTPRNLSNLVLQISVSGTIAIGIVLVLLLGEIDLSV
                     GSVAGATAAVLGVLVVDIGWPWWAAIVAMLAVGTFIGAIQGIWIAVLRVPSFVVTLAG
                     LLIWLGVQLHVLGDAGTLNVFDPRITAIASTFLPHAWGWALALVAVIGYAGTHLVNDA
                     ARRRAGMQAPSLPLLAMQTVAIAVLLLGAVAVLNQANGVPTAGVLLFALVAFFDWLTR
                     RTTFGRHIYAVGGHPEAARRAGINIARVRICVFALSGALTAVGGLILTSRLQAASTQS
                     GGGTLLLEVIAAAVIGGTSLFGGRGNVWGALLGALVIGSVSNGLDLVGQPADVKYMVE
                     GAILLLAVTVDAFSRRRLTSAGR"
     gene            complement(2685..3779)
                     /locus_tag="P5558_05235"
     CDS             complement(2685..3779)
                     /locus_tag="P5558_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HRY23743.1"
                     /translation="MATAVLRRDYSLTGPEADKAVERGLVAAEWYHSRVPRQRMKELM
                     RRRDAPALRDLALWLAALALSGGIAGWLYLQGSWWALPFFLVYWVLYGSTSDARWHEC
                     GHGTAFRTAWINDLVYEVASFMVFRNATVWRWSHARHHTDTIIVGRDPEIVAMRPPHV
                     WKVLLNFLALPNLYYGFKEMFIHSMGRLTPDQATFIPEAERPRVYLRARIYLAIYASV
                     ILLALAFQTWLPLLYVGLPVLLGGWLIMYLALTQHVGLAEDVLDHRLNARTIYMNPLL
                     RFLYLNMNYHVEHHMFPMVPYHNLPALHEELKADMPKPYRSTFEAYREIIATLKRQLR
                     DPGHFVKRELPPSARPFRAELHGHGFAEAG"
     gene            3911..4771
                     /locus_tag="P5558_05240"
     CDS             3911..4771
                     /locus_tag="P5558_05240"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY23744.1"
                     /translation="MPDFPIIDSHVHLYDVEKLSYPWLANVPRINRSYGLADFDAARG
                     PVEVEALVFAEVWVGPGLHLDEAAWVQGLADRDPRLQGMIAHLPLELGPDVVRGDLEQ
                     LLQHPTLRGIRRLIEIELDPRFCLEPGFLAALKLLPRHDLVFDICVKHWCLPFAVELA
                     QRCPDVTFVLDHLGKPGIKHGMWQPWADLMKELGRLPNVICKVSGVITEADHAHWQKA
                     EVVPYLRHAIECFGFDRLMYGSDWTVSELTHPYPEWVRILDEVMDGTSEAERRQLYRE
                     TARRVYRLQG"
     gene            4909..6720
                     /locus_tag="P5558_05245"
     CDS             4909..6720
                     /locus_tag="P5558_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRY23745.1"
                     /translation="MLLLAIATGWPSGARAADATASLADPLAPPDLSSPGATLNTFIA
                     EMEAAVRSYYEGNLGAMHAHAGRAFATLERTIAGGEASFVEMADKSLMLLEVLARVTL
                     PPPDDMPDAAQVAENGLQAWTIPGTELRIASQSDDTGLATGFLFTEDTVQRIPAFYRV
                     IQHLPAKGELRRYQGIVERFRRGPGFAAPAVVTSLVQSLPGQWFILIGELPIWKWTAL
                     ATTLLLAIATYVLGFRLAVRLERRPAETGHRARWAGVARSVLALVLAVVAHFLLLYII
                     GITGLSAAVVGVLLTIVGHLAVAWLIFQLLNIAADFVIRVRELGVASLDTQLVRLVFH
                     LASVLLGLYVLVHMADRLGVPVTPMLAGLGVGGLAVALAVRPTLENVVAGFVLFADKP
                     VKVGEFCLFGDKMGTVEAVGLRSVRVRGIDRTLVSVPNAEFCQMQIVNFTRRDSILLQ
                     SSIGLRYETSADQLRLVLVRLRELLIRHPKIAIEPARVRFVGYGAFSLDLEIFAYVTT
                     RDFNEFLAIQEDINLRIKDIVEGIGAGFAFPSQTLYLERSAGPDPRQSEAAGQTVERW
                     RQENRLPFPNHEEQFRYELADTLDYPPRGSAAAKAAS"
     gene            6806..7951
                     /locus_tag="P5558_05250"
     CDS             6806..7951
                     /locus_tag="P5558_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRY23746.1"
                     /translation="MLSPPSFSWLATEEPGYSFIGTRQQPLARDPAAGSLLGSAVMRG
                     GTGADVLRSEARTSLVLIGGMGDDVLHGSATAQRLLIGGAGDDTYVLRSTGDILSDMA
                     GNDTAILVTELAHALAAGANAVVALAGGMPPAEPWLAPITLELAPGIENLTAIVWERS
                     WSMSFDLVGNEGANRLIGDSFANVLVGNGGDDTIFGHGGDDRLDGGTGDDALNGGDGR
                     DHLTGGAGHDVLSGGAGDDLLEGGAGNDRLDGGPGDDQLLGGDGNDTLVARLGSDLLD
                     GGAGDDLFFIGHRAGAVTLIGGPGADRFVVEARPGDLMLHFADFLAGEDELDLAAWLG
                     TGRMQVTETAIDGAASAWLVTAPERSIALSFASDTLIAAEDNALAIA"
     gene            8114..8599
                     /locus_tag="P5558_05255"
     CDS             8114..8599
                     /locus_tag="P5558_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23747.1"
                     /translation="MNRATIGGLALALVLHLSGGVAIAQAPATVTPQQGQTAEQVAAD
                     QAACESQAMASSGYNPAATAAAPQAAPVMGQRARGAARGAAAGAVREQRTDSSEREVD
                     DLTESAARAGAVAGGMRQRGERREQRRHTQQAEAATASQQSAYFQAFGACMTAKGYSV
                     R"
     gene            8765..9373
                     /locus_tag="P5558_05260"
     CDS             8765..9373
                     /locus_tag="P5558_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY23748.1"
                     /translation="MVRAVRDAVGSEIMVAMDAHWKYANGAIKLAQAPEPFDLLWLED
                     PVPPENIEAQRHVTHSTRTPICTGENLYRKHGYRELIEKQAARIIAPDIPKMGGLAEA
                     KKVADHADLYCIPIAPRNVASPIATVAGSHVCAGINNFLVIEYHAHDVEWWSDLVKEK
                     PPIDKGFIRIEDRPGPGPRLNEEAARAHLKPGSSFFGDKPHG"
     gene            9431..10189
                     /locus_tag="P5558_05265"
     CDS             9431..10189
                     /locus_tag="P5558_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY23749.1"
                     /translation="MRNQLLVKVETDEGLYGWGESGLSGREKAVAGAIDHFAGFVVGK
                     DPMRIGALWQELYRSQHFEGGRVLTAAMSAIDIAHYDNKGKALGMPVYDLLGGRQRDR
                     MPTFATTHAEPGLAMIEQARALVERGWSAMRLSPSGHQSKDVHAPLEHIAATAEWCTR
                     AREALDHEVELGIDDRHRLSVAEAARFCQKMPRGTLDFLEEPSRDETPEAYEALRRMT
                     DIPFAIGDELASKWQFLHGRRARSRADEATPEGG"
     gene            10413..11441
                     /locus_tag="P5558_05270"
     CDS             10413..11441
                     /locus_tag="P5558_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY23750.1"
                     /translation="MPSFISRLLGSAMVVAMATTGAMTVTSASAASGDHEDELVVYAS
                     HPSEMVDFFAQAFGEKYGVSVTTVKAGTGELLNRIRAEKDRPSADIMWGGFADTGASA
                     PDLFDPYESPELANIEPDMIDPKGYNSPFGASLMVIMYNSELLPAEEAPQRWEDLADP
                     KWKGKVVHADPSKSSSALAALSTWLMIYGRDDAAWDLVERMTGNMNIVLKSSLVFQQV
                     GRGEYPVGVTYEEAAFNYVMAGTGGIVYPADGTLAQPEGTFIVKSAPHPKAARLFADY
                     LMSEEAQQALVANFPGRRPTRIGVDTHPQMLPLSEINVIDYDSEWASANRDEILERMQ
                     QIIIKTQQ"
     gene            11554..>11855
                     /locus_tag="P5558_05275"
     CDS             11554..>11855
                     /locus_tag="P5558_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23751.1"
                     /translation="MTTAVSIDSVTKRFDATTAVDDVTIDIAKGEFFTLLGSSGCGKT
                     TLLRIIAGFLRQTAGTIRFDDKPIDDVPAHRRNTGMVFQNYAIFPHLSVFDNIAYGL"
     assembly_gap    11856..11955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11956..12794
                     /locus_tag="P5558_05280"
     CDS             <11956..12794
                     /locus_tag="P5558_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23752.1"
                     /translation="NYAIFPHLSVFDNIAYGLRARRCAKAEVEARVGQMLELIQLAAL
                     RHRRPRELSGGQQQRVVLARAMVIQPDVLLMDEPLSNLDTAMRVHLRAEIRALQRQVG
                     ITTIYVTHDQEEALLMSDRVAVMSHGRVEQIATPLEIYNAPETRFVAGFVGECNMLPG
                     KVAEVLDGTATVALGEGGLLIVDGVSGVASGEARTVAFRPQDVELQPVPDDDMRGLCG
                     RLADIQFLGGTVRFVVDIGAGAIVHVAAQRRTGAAIPVLGGRYRLDIPAHTVRLFEPT
                     PA"
     gene            12805..14472
                     /locus_tag="P5558_05285"
     CDS             12805..14472
                     /locus_tag="P5558_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY23753.1"
                     /translation="MTGAMVAARRLLGFWPSVTAGMLVVTAALLLLPVFNIVGLSFSG
                     KEDAAGVLQNYRMFFSEPYYYRTLVNSFIVSISATVLALLVGVPAAYFTSRFHIWMRS
                     LIRAAVVLIFVSPPFIGSYSWIILLGRNGLVTTTLAEYGISLPSIYGAQGIIFVFTLH
                     YFPFVFLLVSSGLKSIDQSIEDAARNLGSSELATFRKVIAPLLAPSISTGALLVFIAC
                     FTDLGTPIILGERFRVLPVLIYGEFVNDFGGRPVLASTLSVLLLLITTSALLLQRWYA
                     SRNAFGTAAVRPLTLQEQPFKKRLLAALFVYTLVGVALLPLANIIVSSFLKANGPVLL
                     AEFTLDNYRRILGTIGRPLWNTIFFTTTATLLCAVFGTMIGYIIVRRPGRISGFLDTI
                     IMFSFAVPGIVLGVGLVVTYHHQPFAMTGTALILILAYFIRRLPFSVRSSVGMLQQLS
                     RDTEEASVNLGAGPAMTFLKITVPLLSVAILSGVLLTWSNTIRELSATLILQSGRTVT
                     VSLEIFNEVVNGNFGIASALGTVLILLTFLPLSVLFTAMGKREDALV"
     gene            14537..15502
                     /locus_tag="P5558_05290"
     CDS             14537..15502
                     /locus_tag="P5558_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HRY23754.1"
                     /translation="MASRQRIGSGRGSALNDDIVLEAAWLYYYEGRSQHEIAGTLGVS
                     RSSIVGYLQQARETGMVEIRLDAGVFSRHRLSHALSQRYGLAETYVVPGDTFSLERVA
                     VGAGRRLPDLIDPGDVLGVAWGQTIFNLSQNIVPRRIDRLTVIQMVGSMSSPYGFDAE
                     ICSSHIALSLGGRCINLHAPAVLSTSAMAEALRQEPIIRAQLEQVSRIGKAVFAVGSC
                     NETSHIVQAGIASVDDLARYRRHGAVGLVCGRFIDAAGNHIHGHLDERLIGVTPDCLR
                     GLRMGILVSSGADKIEGIHAALRGGYATHLVTDIPTAEGILTLSR"
     gene            complement(15530..16024)
                     /locus_tag="P5558_05295"
     CDS             complement(15530..16024)
                     /locus_tag="P5558_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY23755.1"
                     /translation="MAEVMRELLDRRQIEDLLVDYCRHLDRMELDALAALFTTDCRVT
                     YGAEPRLAAVGREALARSLARMWRWRRTAHHLANVRVWLDSADTARAESCVHAWHERP
                     DGRTATVYGRYLDSLRRTPEGWRIAERRMDMNGADANFHVPIPQAPRQPPPPGWQPPE
                     GLDG"
     gene            complement(16017..16901)
                     /locus_tag="P5558_05300"
     CDS             complement(16017..16901)
                     /locus_tag="P5558_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRY23756.1"
                     /translation="MAEFPQPWQMPESEWRRRVGRVRAGRALAPRQWPGGAICAVALS
                     FDCDHETFELGRGGSAIGRLAWGEFGRRVGVPRILDVLARLEVRASFFVPAVAGLIDP
                     DEPRRIVGAGHEVAMHGWIHENTSLLGEAEERELMLRARDTLEGLTGMPPVGFRAANW
                     DLSPSTIPLVAELGLAYDSSLMADEACYELVCDGEPTGVVEVPVEWLRDDAVYFLFNR
                     EPATRPWTPPDDVLAIFRRELDAAMAEGGVFQLVMHPFVIGYRSRIWILEALIEQAKG
                     RAWFATHAELAQWVREHG"
     gene            17057..18088
                     /gene="dctP"
                     /locus_tag="P5558_05305"
     CDS             17057..18088
                     /gene="dctP"
                     /locus_tag="P5558_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY23757.1"
                     /translation="MARIDLLARASAIVAATGLMVGAFGATAQESITWRVQTGWLPGN
                     TLYDSVVELAERIDLQSEGRLKIEVLPAGAVVALNQTIDSVKAGIIDGHISYAAVYAG
                     LDPGFAPLGDIPGAYDDRFQTIEFMYYEGGLELLREAYAQFGLHTIGVGTGSWEAVPS
                     RVPLESWDDFEGVKLRTPPGIGSLIWERLGAVPIVMPQTEVFSALEKGVIDAADDGSL
                     SYNFQVGDYDIVDFTLVNSPHSNGVWDFTVNADRWAELPNSIKSMLEINMREIMLRNV
                     MNALRQDQEVLAKADEIGLTLFELTPEDRAKYRAIAVEVWDEWATKSELSKKAVDAHK
                     AFLTKKGLL"
     gene            18162..18638
                     /locus_tag="P5558_05310"
     CDS             18162..18638
                     /locus_tag="P5558_05310"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY23758.1"
                     /translation="MITRFNEQLGLALSYVYALVAVLTFGEVCARYFFNSPTQWTIEV
                     VVLLAAMHYLIAGPQAYASEGHIRITVLYDRLPLPARRALTVLERLVVATTAGLVGWW
                     ALGQAQSALAIGERTGSNLNTYSPTILKVVLAIALGLLALQALAHLVRDRGRAHGG"
     gene            18628..19941
                     /locus_tag="P5558_05315"
     CDS             18628..19941
                     /locus_tag="P5558_05315"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY23759.1"
                     /translation="MAAEVATYLVLGLLFFFIAIGTPLAIATMATAIIVGWSLFGFNS
                     ILLIANRIYDASTNYVLIAVPLFILMGVLMERGGIARRLFTVLHIWSARLPGGMAVGC
                     VFAGAIMAAMVGIVGAEIITLGLVALPAMLARGYDKQLTLGVICASGSLGAMIPPSLV
                     LVFYGLIAGESIARLFAAALVPGLVLALSYAIYIVVRAKTRPHLAPPPSQDELEMPIA
                     EKLALGRALFLPALIITGVLGSIYFGIAAPTEAAAIGVAGALLAVIVSGEFRLADLHH
                     TVVQTGKAIGPVIWVFFGANSLISIYSLSGGVKYLAGLIAAVPLPPLGVLLLIVAILF
                     LLGMVMDWIGIAILTMPVFLPVVGALGYDPIWFGVIFCISMQIAYLTPPIGASAFYLK
                     SVAPPDVTLGEIFMSIWPFVGLQLVVLALVIAFPALALWLPAQMG"
     gene            complement(19945..20730)
                     /locus_tag="P5558_05320"
     CDS             complement(19945..20730)
                     /locus_tag="P5558_05320"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23760.1"
                     /translation="MAEGALAGRTAIVTGAASGIGRGIAERFALEGASVLCADLDGAA
                     AERVAMALGEAGHAAKASAVDITDEASVAAMVDAALAWTGRLDSVAANAGIMVEGDIL
                     ALSLAQWNRALAVNATGSFLTARAALPHLARSGAGTLVFTASTVGLVGMKGTAAYSAS
                     KGAVVALTRQLAADFAGRGVRVNAVAPGAVRTPLSESQFRARATDEAHFEALLAQVIG
                     RYPLPRWGSPAEIAEVVLFLASERSAWMTGQIIPVDGGLLEIR"
     gene            complement(20723..21511)
                     /locus_tag="P5558_05325"
     CDS             complement(20723..21511)
                     /locus_tag="P5558_05325"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY23761.1"
                     /translation="MVVQNVIELSCWERLAEATAGIGEARLFPPEPPLDAQGRMPDGF
                     GAQFRASLARLEARLRAAGGSLDHVLRAEIRVDRSGPEAYGALQAAFLAGFAAMPRPA
                     RCVSAATGLAHGGLVEVGAVAATGAPPGSGPDAAIIAADGPAPCAPFPHARAHEGGVW
                     VTAQVGSDPASGSLAPVPGGSAVQLAQAMSNLEAILRASGSSLERVVHLRLQYTAASE
                     RDLLARLIKERPLPFCAVELAEVPFIPTAQAGVTVKVDAVARLG"
     gene            complement(21522..22220)
                     /locus_tag="P5558_05330"
     CDS             complement(21522..22220)
                     /locus_tag="P5558_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRY23762.1"
                     /translation="MLKFYYNTAPNPIKVALFLEEAGLDYEAVPIDTRRGDQHRPEYL
                     AINPNAKVPAIVDGEVVVFDSGAILLYLAEKTGQFLPAQTPVARGELLSWLLFVASGV
                     GPYSGQAVHFRHFAPVSDPYPRERYAFEARRHWKIVDDRLADRPYMLGDSYTIVDMSV
                     WGWATRIPFMLGDEAMAEFPHLGRLVDEIGARPAAKRALAIGERHEFKAELDEDAVRN
                     LYPHLHATRAKAGS"
     gene            22429..22866
                     /locus_tag="P5558_05335"
     CDS             22429..22866
                     /locus_tag="P5558_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRY23763.1"
                     /translation="MRDERFEVEPFTGSLGAAIRGIDLVADDHAMIDEARRFGPFGGN
                     PVHTPVEGYEDIMRFAREPDDSGKVIGEEGHMDLAWLARPPGIVMLVGEEVPPVGGDT
                     CFTSLEEACQSLSSRMVAMGAELAGIHSGRGVFAINAASHASA"
     gene            22930..23034
                     /locus_tag="P5558_05340"
     CDS             22930..23034
                     /locus_tag="P5558_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23764.1"
                     /translation="MAERRNIAEATALFLVSEEASYVTGATLYVNGGF"
     gene            complement(23018..>24048)
                     /locus_tag="P5558_05345"
     CDS             complement(23018..>24048)
                     /locus_tag="P5558_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23765.1"
                     /translation="SPLHARHVADGAVLEAVAGHERPLWFTGNGSAAAELAAARGAVV
                     RIERSADAKLLLAGPDAGAMVRRLSGAVGVDRDDAAVLAPMLDARGGVVACPWAVRLD
                     AASWLLLADPDGLGWLRRAVQPLPTERVAVVDAGAQWAALDLVGPAAEALLARVADDA
                     PLPMPGRARGAGLALGQGVIAHLAEAHWRLLVPADMAVHVDEALRREGARPAGHLVDE
                     TLRILAGRPRAGIDATPVHGALAAGLVGSLELGRDFVGRAAVAGEGDSAPRARLVAAR
                     VEAAAIEPLEPVLRRGENVGWISSAGLAVDGRVPVLALVEGPLDGLETLAAGEKVGRL
                     VGPSRSRH"
BASE COUNT         3616 a         8630 c         7976 g         3726 t
ORIGIN      
        1 ctggtcgaat ccgtctggac gcccggctgg gatccgtcga aggcccagca gcagatggag
       61 cagatcctga ccaggacgaa caacggggtg gatgcggtcc tgtccgccaa tgacggcatg
      121 gcgggtggcg tcatcgcggc gctccaggcc cagcaaatgg ctggtgagat ccccgtgacc
      181 gggctggatg gcacgctgaa cggcctgcag ctcattctgc tgggcaggca gtcgatgacg
      241 gtatggcggt cgctcaagga ccagtcgggg aaggccgccg cgatcaccgc ggcgctcctg
      301 aagggcgaga agccggccga cagcatgttc gagggcgcca cgatgaacaa cggcgcaacc
      361 gagatcccgt gggccaaggt gacgcccatc gtcatcacgc tcgacaatat ggagctcgag
      421 atcgaggacg gcgccgtcac cagggaagcc ctttgccggg gcatgccggc cgtcgggcct
      481 tgcaagccgt agcgacagcc cgaggtgaca agcggcaggg ctgacggcgc tggatcggcc
      541 gttctgcagc tcgagggtat ctcgaagcgg ttcggcgccg tcgaggcctt gtcggaggtc
      601 gatttcgagg tccgcgcggc cgaggtcgtg gcgctggccg gcgacaacgg cgccggcaag
      661 tcgaccctgg tgaaggtgat cgccggcacc catgcgccgg atggcggcgt catccgcttc
      721 gatggcgtgg aggtgagcat ccgctcaccc cgccacgccg cgtctctcgg catcgagacg
      781 gtctaccagg acctcgccct gtgcgacaat ctggacgtcg tcgccaacat gtatctcggc
      841 cgcgagacgg tcggccgacc ccgcttcctg caaaggctgt cagagctcga catggagcgg
      901 cacgctctcg acgtcctgcg ccgcctcaac gtcacgacga tccgcagtgt ccgcgccgcg
      961 gtcgccactc tttcgggcgg ccagagacag tcggtggcca tttcccgagc ggtgatgtgg
     1021 aactccaaga tcgtgctcct ggacgagccg accgcagcac tcggcgtggc gcagacgcac
     1081 gaggtcctgg agctgatcaa gcggttgcgg gcgcaaggtc tgggtgtggt cctgatctcg
     1141 cacaacctgc acgacatgtt cgaggtggcg gaccggatcg tcgtcctgcg cctcggccgc
     1201 aacgcggcgg cgttcaggtg ccgcgaaacg acccccgagg aggtcgtggc ggcgatcacc
     1261 ggagcgcgcg aggcagccgc gcgcgaggcc gtgtgctgaa gcaggctcag gacgcgcgcg
     1321 accgcatgag cggcagccgg gccggtggcc gagctcgcac cggcgcatcc ctgaccacgc
     1381 tcgctcgcgg cctgcgattg cgctcggatt ccagcgagct cggggttctc cccatcgtgc
     1441 tcgggctcgc gatcatctgg acgtttttcc aactgcagga cgcgcacttc ctgaccccgc
     1501 gcaacctttc caatctcgtc ctgcagatct ccgtctcggg caccatcgcc atcggcatcg
     1561 ttctcgtcct gctgctcggc gagatcgacc tgtccgtcgg ctcggtcgct ggcgccaccg
     1621 cggccgtttt gggcgtgctc gtcgtcgaca tcggctggcc ctggtgggcg gcgatcgtgg
     1681 cgatgctcgc ggtcggcacc ttcatcggcg ccatccaggg aatctggatc gcggttctgc
     1741 gcgtcccgtc cttcgtcgtg acgctggccg ggctgctcat ctggctcggc gtccagctcc
     1801 acgtcctggg ggatgccggg acgttgaatg tcttcgatcc gcgaatcacg gccatcgcct
     1861 cgaccttctt gccgcacgcc tggggctggg ccctcgcgct ggtcgccgtc atcgggtacg
     1921 ccggcacgca tctcgtcaac gacgcggccc gtcgccgggc cggcatgcag gcgccgtcgc
     1981 tgccgctcct ggccatgcag acggtcgcca tcgccgtcct gctgctcggg gcggttgctg
     2041 tcctcaacca ggccaatggt gtgccgacgg ccggtgtcct gctcttcgcg ctcgtcgcct
     2101 tcttcgactg gctgacccgc cgcacgacgt tcggtcgcca catttatgcg gtcggcggcc
     2161 accccgaggc ggcccgccgg gccggcatca acatcgcgcg cgtccgcatc tgcgtcttcg
     2221 cgctgtcggg ggcgttgacc gccgtcggcg gcctgatcct gacctcgcgc ctgcaggcgg
     2281 cgagcacgca gtcgggcggc ggcacgctgc tgctcgaggt catcgccgcc gcggtgatcg
     2341 gcggcacgag cctctttggt ggccggggca atgtctgggg cgccctgctc ggtgccttgg
     2401 tgatcggcag cgtctcgaac ggcctcgacc tcgtcggcca gcccgccgac gtgaaataca
     2461 tggtcgaggg cgccatcctg ctgctcgccg tcacggtcga tgccttctcc cgccgccggc
     2521 tcacctccgc cgggcgataa cgctcgcgcg gcatccatct cgtttgcatc cgcacccacc
     2581 cgccagggtg cctcgaagcg gatagcgacg cccggctcgg cgggcaagag acgggtctcg
     2641 ggaatccagc ctgtcccacc gtcgacgcgg atcgccggat ggagtcaccc cgcctcggcg
     2701 aagccatgcc cgtgcagctc cgcccggaag ggccgggccg agggcggcag ctcgcgcttg
     2761 acgaaatggc ccgggtccct gagctggcgc ttcagcgtgg cgatgatctc gcgataggcc
     2821 tcgaatgtgc tccggtaggg cttcggcatg tcggccttca gctcctcgtg cagggccggc
     2881 aggttgtggt acggcaccat ggggaacatg tggtgctcca cgtggtagtt catgttgagg
     2941 tagaggaagc gcagcagcgg gttcatgtag atggtgcgcg cattcagccg gtggtcgagc
     3001 acgtcctcgg cgagcccgac atgctgggtc agcgccagat acatgatgag ccagccgccg
     3061 aggagcaccg gcaggccgac atagagcagc ggcagccagg tctggaaggc cagcgccagg
     3121 aggatcacgg aggcgtagat ggcgaggtag atgcgcgccc tgaggtagac cctggggcgc
     3181 tcggcctcgg ggatgaaggt cgcctggtcg ggggtcaacc ggcccatgct gtggatgaac
     3241 atctccttga agccgtagta gaggttcggc agggccagga agttcaagag caccttccag
     3301 acatgcggcg ggcgcatggc gacgatctcg gggtcgcgcc cgacgatgat ggtgtcggtg
     3361 tggtggcggg catggctcca gcgccagacc gtcgcattgc ggaacaccat gaaggacgcg
     3421 acctcgtaga cgagatcgtt gatccaggcg gtcctgaacg ccgtgccgtg gccgcactcg
     3481 tgccaacggg cgtccgaggt ggagccgtag agcacccaat agacgaggaa gaacggcagc
     3541 gcccaccagc ttccctggag atagagccag ccggcaatgc caccggagag cgccagggcg
     3601 gcgagccaga gggcgaggtc gcgaagtgcc ggggcgtcgc ggcgacgcat cagctccttc
     3661 atccgctggc gcgggacccg gctgtgatac cactcggcgg cgacgaggcc gcgctcgacc
     3721 gccttgtcgg cctcggggcc ggtgaggctg tagtcgcgtc gaagaacggc ggtggccaag
     3781 atctcctcct ttgcaaagag ccggccgcga tgcagcatag ggcgacaagg cccagaattc
     3841 aaagcaggac agggcttgcg agcggcgcgg tcggccgcta ccgcaggctg ccaggaatcg
     3901 agagggcgcc atgccggact ttccgatcat cgacagccac gtccacctct acgatgtcga
     3961 gaagctgtcc tatccgtggc tcgccaacgt gccgcggatc aatcgcagct acgggctggc
     4021 ggacttcgac gcggcgcgcg ggccggtcga ggtcgaggcc ctggtcttcg ccgaggtctg
     4081 ggtcgggccg gggctgcacc tggacgaggc ggcctgggtc cagggcctgg cagaccggga
     4141 tccgcgcctg caggggatga tcgcccacct cccgctggag ctcggtccgg acgtggtcag
     4201 gggcgacctc gaacagctcc tgcagcaccc gaccctgcgc ggcatccgcc ggctcatcga
     4261 gatcgagctc gacccgcgct tctgcctgga gcccggcttc ctcgccgccc tgaagctgct
     4321 gccgcggcac gacctcgtct tcgacatctg cgtcaagcac tggtgcctgc ccttcgccgt
     4381 cgagctggcg cagcgctgcc ccgacgtcac cttcgtgctc gaccacctgg gcaagcccgg
     4441 catcaagcac ggcatgtggc agccctgggc cgatctgatg aaggaactgg gccggctgcc
     4501 caacgtgatc tgcaaggtct cgggcgtgat caccgaggcc gaccatgccc actggcagaa
     4561 ggcggaggtg gtcccctatc tccgccacgc catcgaatgc ttcggcttcg accgcctgat
     4621 gtacggcagc gactggaccg tctcggagct cacccacccc tacccggaat gggtccgcat
     4681 cctcgacgag gtgatggacg gcacgagcga ggcggagcgg cgccagctct accgcgagac
     4741 ggcaaggcgg gtctaccggc tccagggctg agctgccgcc ggccgccgcg ggttgccttg
     4801 ccggccactt cccggccatc ctgtccccga tgcggttgat gcggaggtga cagggtgacg
     4861 acgaggtcga cgatagcggc tcatgggggc cgggcagccc ggttgctggt gctcctgctg
     4921 gccatcgcca cgggctggcc gtccggagcg cgggccgccg acgccaccgc cagcctcgcc
     4981 gatcccctcg caccgcccga tctctccagc ccgggggcga cgctgaacac cttcatcgct
     5041 gagatggaag ccgccgtccg cagctactac gagggtaatc tcggcgccat gcacgcccac
     5101 gccggacgcg cctttgcgac cctcgagcgg acgattgccg gcggcgaggc gagcttcgtc
     5161 gagatggccg acaagtccct gatgctgctc gaggtgctgg cgcgggtgac gctcccgccg
     5221 ccggacgaca tgcccgacgc cgcgcaagtc gcggagaacg ggctgcaggc ctggaccatt
     5281 cccggcaccg agctgcgtat cgccagccag tcggacgaca ccggccttgc caccggcttt
     5341 ctgttcaccg aggacacggt gcagaggatt ccggcgttct accgggtgat ccagcacctc
     5401 ccggccaaag gcgagctccg ccgctatcag ggtatcgtcg agcgcttccg ccggggcccc
     5461 ggcttcgcgg cgccggcggt ggtgaccagc ctcgtccaga gcctgccggg ccaatggttc
     5521 atcctgatcg gcgagctgcc gatctggaaa tggacggcgc tggcgacgac gctgctgctc
     5581 gcgatcgcga cctatgttct ggggttccgg ctcgcggtgc ggctcgagcg ccggccggcg
     5641 gagacgggcc accgcgcccg ctgggcgggt gtcgcgcgca gcgtgctggc cctggtgctg
     5701 gccgtcgtcg ctcacttcct cctcctctat atcatcggga tcaccggtct gtccgcggcc
     5761 gtggttgggg ttctcctgac gatcgtcggt catctcgctg tcgcctggct catcttccag
     5821 ctcctgaaca tcgccgccga cttcgtgatc cgggtgcgcg agctcggcgt ggccagcctc
     5881 gacacgcagc tcgtgcggct ggtcttccac ctggcgagcg tgctgctcgg cctctacgtc
     5941 ctggtgcaca tggcggaccg gctcggcgtg ccggtgacgc cgatgctcgc cgggctcggc
     6001 gtcggcggcc tcgccgtggc gctggccgtg cggccgacgc tcgagaacgt cgtcgccggg
     6061 ttcgtcctct tcgccgacaa gccggtcaag gtcggcgaat tctgcctgtt cggcgacaag
     6121 atgggcacgg tggaggcggt cggcctgcgc tccgtccggg tgcgcggcat cgaccggacc
     6181 ttggtctccg tgccgaacgc cgagttctgc cagatgcaga tcgtcaactt cacccgccgc
     6241 gacagcatcc tcctgcagag cagcatcggc ctgcgctacg agacgtcggc ggaccagctc
     6301 cgcctcgtgc tggtccggct gcgcgagctc ctgatccgtc atcccaagat cgccatcgag
     6361 ccggcgcggg tgcgcttcgt cggctacggg gcgttctcgc tcgatctcga gatcttcgcc
     6421 tatgtcacga cccgcgactt caacgagttc ctggcgatcc aggaggacat caacctgcgc
     6481 atcaaggaca tcgtcgaagg cattggcgcc ggcttcgcct tcccgtcgca gacgctctac
     6541 ctggagcgct cggccggccc cgaccccagg cagtccgagg ccgccggcca gacggtcgag
     6601 cgctggcgcc aggagaaccg cctgcccttc ccgaatcacg aggagcagtt ccgctacgag
     6661 ctcgccgaca cgctcgacta cccgccgcgg ggctctgcgg cagcaaaggc cgcctcatag
     6721 gccccgggag tggaggcgct aaccgggcgt taaccacgta gccgccatca tgccgcccca
     6781 actgtcatga tgggcaaccg gctgcatgct ttcaccgcca tccttttcct ggctcgcgac
     6841 cgaggagccg ggctacagct tcatcggcac ccggcagcaa ccgctcgccc gagaccctgc
     6901 cgccggcagc ctcctcggat cggccgtgat gcgtggcggc accggcgcgg acgtcctgcg
     6961 cagcgaggcc cgaacgtcgc tcgtcctgat cggcggcatg ggcgacgacg tcctgcacgg
     7021 ctcggccacc gcccagcgct tgctgatcgg cggcgccggc gacgacacct acgtgctccg
     7081 gtcgacaggc gacatcctca gcgacatggc cggcaacgac acggcgatcc tggtgaccga
     7141 gctcgcccat gcgctggcag ccggggcaaa cgcggtcgtg gctcttgccg ggggcatgcc
     7201 gccggccgag ccatggctcg cgccgatcac cctggagctg gcgccgggca tcgagaacct
     7261 gaccgccatc gtctgggagc ggagctggtc catgagcttc gatctcgtcg gcaacgaggg
     7321 cgccaaccgg ctgatcggcg acagcttcgc caacgtcctc gtcggcaatg gcggcgacga
     7381 cacgatcttc ggccatggcg gcgacgaccg gctcgacggc ggcacgggcg acgatgccct
     7441 gaatggcggc gatggccggg accacctcac gggcggtgcc ggccatgacg tcctctcggg
     7501 cggggccggt gacgacctgc tcgagggtgg ggccggcaac gaccggctcg acggcgggcc
     7561 tggcgatgat caactgctcg gcggcgacgg caatgacacg ctggtcgccc gcctgggcag
     7621 cgacctcctc gacggcgggg ccggcgacga cctcttcttc atcggccatc gggccggagc
     7681 cgtgacgctg attggtggcc ccggggccga ccgcttcgtt gtcgaggccc ggcccggcga
     7741 tctcatgctg catttcgccg atttccttgc cggcgaggac gagctcgacc ttgccgcctg
     7801 gctcggcacc ggccggatgc aggtcaccga gaccgccatc gacggcgccg cctcggcttg
     7861 gctggtcacc gcgccggagc gctcgatcgc cctctccttc gccagcgaca ccctgatcgc
     7921 cgccgaggac aatgcgctgg ccatcgcctg aacgagccga acgaatagct cgcccgctga
     7981 ctccggggac cggtgcgaca gtacgtgctc caccacaccc gcggctgcct gctatgctcg
     8041 gtgcgcccgt ccgtcccggc gggcggggcg gcgccggaac ccggcgcgcc gatcgactga
     8101 aggaagccat gcgatgaatc gtgcgacgat cggcggcctg gccctggcgc tggtgctcca
     8161 cctctcgggc ggtgtcgcca tcgcccaggc gccggcaacg gtcacgcccc agcagggcca
     8221 gacggccgag caggtcgcgg ctgatcaggc agcctgcgag agccaggcaa tggcgagctc
     8281 gggctacaat ccggcggcca cggccgctgc gccacaagcc gcgcccgtca tgggtcagcg
     8341 ggcgcgaggt gcggcgcgcg gtgctgcggc cggcgcggtg cgcgagcagc gaaccgattc
     8401 gagcgagcgc gaggtggacg acctgaccga gagcgccgcc agggccggtg ccgtggccgg
     8461 cggcatgcgc cagcgcggcg agcgccgcga gcagcgtcgc cacacccagc aggccgaggc
     8521 cgccaccgcc tcgcaacagt ccgcctactt ccaggccttc ggggcctgca tgacggcgaa
     8581 aggctactcg gtccggtagg gtcgaagagc gaaccggcga atcggcccgc tgccttcagc
     8641 ctgccgattg ctcccggctc gcgactcgtc ggcatgggct tcagaagtgc atgcgcgccg
     8701 cggtggtggt ggctgccgca cgagtgcagg cgatccttga cgctgccggg cttggatggt
     8761 cgacgtggtg cgcgccgtcc gggatgccgt cggctccgag atcatggtcg ccatggacgc
     8821 ccactggaaa tacgccaacg gcgcgatcaa gctcgcccag gcgcccgagc ctttcgacct
     8881 gctctggctc gaggacccgg tgccgcccga gaacatcgag gcccagcgcc atgtcaccca
     8941 cagcacccga acgccgatct gcaccggcga gaacctctac cgcaagcacg gctaccgcga
     9001 gctgatcgag aagcaggcgg cccggatcat cgccccggac atccccaaga tgggcggcct
     9061 cgccgaggcc aagaaggtcg ccgaccacgc cgacctctat tgcatcccga tcgccccgcg
     9121 caacgtggcg agcccgatcg ccaccgtcgc cggcagccat gtctgcgccg gcatcaacaa
     9181 cttcctcgtc atcgagtacc acgcccacga cgtcgaatgg tggagcgacc tcgtcaagga
     9241 gaagccgccg atcgacaagg gattcatcag gatcgaggac cggccgggcc ccggcccgag
     9301 gctgaacgag gaggcggccc gcgcccatct gaagccgggc tcgagctttt tcggcgacaa
     9361 gccccatggc tgaacggcgg gtgaagcagc gaggatcacc gcgcccgagc ccttccccgc
     9421 ctgggtcggc atccgcaatc agctcctcgt caaggtcgag accgacgagg gcctctacgg
     9481 ctggggcgaa tccggcctct ccggccgcga gaaggcggtc gccggggcca tcgaccactt
     9541 tgccgggttc gtggtcggga aggacccgat gcgcatcggc gccctttggc aggagctcta
     9601 ccggagccag catttcgagg gcgggcgggt gctcaccgcc gccatgtcgg ccatcgacat
     9661 cgcgcactac gacaacaagg gcaaggcgct gggcatgccc gtctacgacc tcctcggcgg
     9721 ccggcagcgc gaccgcatgc cgaccttcgc cacgacccat gcggaaccgg gcctggcgat
     9781 gatcgagcag gcgagagccc tcgtcgagcg cggctggtcc gccatgcggc tctcgccctc
     9841 gggccaccag tcgaaggacg tccacgcgcc cctggagcac atcgccgcca cggccgaatg
     9901 gtgcacaagg gccagggaag cgctcgacca cgaggtcgag ctcggcatcg acgaccgcca
     9961 ccgtctctcg gtcgccgagg ccgcgaggtt ctgccagaag atgccgcgtg gcactctcga
    10021 cttcctcgag gagcccagcc gcgacgagac gcccgaggcc tacgaggcac tccgccgcat
    10081 gaccgacatc ccctttgcga tcggcgacga gctcgcctcg aaatggcagt tcctgcatgg
    10141 acgtcgagct cgatcccgag cggacgaagc cacacctgaa ggaggatgac gcgggcttct
    10201 tcgactgaag ctcccgccgc cgatcacacg gaccccatcg gtcgtcagat gacaagcgcg
    10261 atccacgacg cggcccttgc gcaagggtgc caaatgtgga gccgccgaca attgtcaata
    10321 ccgattgaca ggtgtcgaaa ccgcctccta ggctgggcca tggatcatcg atcggccaca
    10381 gccgtggccc cacaacggga ggcgcaaccc tcatgcccag cttcatctcg agactgctcg
    10441 gatcggcgat ggtcgttgcc atggcgacga ccggtgccat gacggtcacc agtgccagtg
    10501 cggcaagcgg cgatcatgag gacgagctgg tcgtctatgc ttcccatccg tcggagatgg
    10561 tcgatttctt tgcccaggcg ttcggcgaga aatacggcgt cagcgtgacc acggtgaagg
    10621 ccggaaccgg cgagcttttg aaccgcatcc gggccgagaa ggaccggccg agcgccgaca
    10681 tcatgtgggg cggcttcgcc gacaccggcg cctcggcgcc cgacctgttc gatccgtacg
    10741 aatcgcccga gctcgccaat atcgagcccg acatgatcga ccccaagggc tacaacagcc
    10801 cgttcggcgc cagcctcatg gtgatcatgt acaattcgga gctcctgccg gccgaggagg
    10861 cgccccagcg ctgggaggac ctcgccgatc ccaaatggaa gggcaaggtg gtccacgccg
    10921 acccctcgaa gtcgagctcg gcgctcgccg cgctcagcac ctggctgatg atctacggcc
    10981 gcgacgacgc cgcctgggac ctggtcgagc gcatgaccgg gaacatgaac atcgtgctca
    11041 agtccagcct cgtcttccag caggtcgggc gcggcgagta tcccgtcggc gtcacctacg
    11101 aggaggccgc cttcaactac gtgatggccg gcacgggcgg catcgtctac ccggcggacg
    11161 gcacgctggc ccagcccgag ggaacgttca tcgtcaagtc ggccccccat ccgaaagcgg
    11221 cccgcctgtt cgccgactac ctcatgtccg aggaggccca gcaggcgctg gtggccaact
    11281 tccccggccg gcggccgacc cggatcggcg tggacacgca cccgcagatg ctgccgctca
    11341 gcgagatcaa cgtgatcgac tacgacagcg agtgggcgag cgccaaccgg gacgagatcc
    11401 tggaacggat gcagcagatc atcatcaaga cgcagcagta agaccgccat tgcagccgat
    11461 cgacccccgg ccgcgaaccg ctgccgccgg gggtgctgcc gtcaccttgt ctttccgcca
    11521 tcccgcccat cgacccgcgg cctgcatgcg ctcatgacga cagctgtctc catcgattcc
    11581 gtcaccaagc gcttcgacgc gaccaccgcc gtcgacgatg tcaccatcga catcgccaag
    11641 ggcgagttct tcacgctgct cggctcgagc ggctgcggca agaccacgct gctccgcatc
    11701 atcgccggct tcctgcgcca gaccgccggc accatccgct tcgacgacaa gccgatcgac
    11761 gacgtgccag cacaccggcg caacaccggc atggtcttcc agaactacgc gatcttcccg
    11821 cacctctcgg ttttcgacaa tatcgcctac ggcctnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnagaac tacgcgatct tcccgcacct ctcggttttc gacaatatcg
    12001 cctacggcct cagggcgcgg cgctgcgcca aggccgaggt cgaggcgcgg gtcggccaga
    12061 tgctcgagct gatccagctc gcggcgctga ggcaccggcg cccgcgcgag ctctccggcg
    12121 gccagcagca gcgcgtggtt ctcgcccgcg ccatggtcat ccagcccgac gtcctgctca
    12181 tggacgagcc tttgtcgaac ctcgacacgg cgatgcgcgt gcatctgcgc gccgagatcc
    12241 gcgccctgca gcgccaggtc gggatcacca cgatctacgt gacccatgac caggaggaag
    12301 cgctcctgat gtccgaccgc gtcgccgtga tgagccacgg ccgggtcgag cagatcgcca
    12361 cgccgctcga gatctacaac gcccccgaga cgcgcttcgt cgccggcttc gtcggcgagt
    12421 gcaacatgct gcccggcaag gtggccgaag tgctcgacgg gacggccacg gtggcgctcg
    12481 gcgaaggggg gctgctcatc gtggatggcg tttccggcgt cgcctcgggc gaggcccgca
    12541 cggtggcctt ccgcccccag gacgtagagc tgcagcccgt gcccgacgat gacatgcgcg
    12601 gcctatgcgg ccggctggcc gatatccagt tcctcggcgg aacggtgcgt ttcgtcgtcg
    12661 atatcggcgc cggcgccatc gtccacgtcg ccgcgcagcg ccgcaccggt gccgccatcc
    12721 cggtgctcgg cgggcgctac cgcctcgaca ttccggccca cacggtccgg ctcttcgagc
    12781 cgaccccggc atgacccgga cgccatgacc ggagcaatgg tggcggcccg gcggctgctc
    12841 ggcttctggc cgtcggtcac cgccggcatg ctggtcgtca cggcagccct gctgctgctg
    12901 ccggtcttca acatcgtcgg cctgagcttc tcgggcaagg aggacgccgc cggcgtgctc
    12961 cagaactacc ggatgttctt ctcggagccg tattactacc gcaccctggt caacagcttc
    13021 atcgtgagca tcagcgccac cgtgctggcg ctcctggtcg gcgtgcccgc cgcctacttc
    13081 accagccgct tccacatctg gatgcgcagc ctgatccgtg ccgccgtggt gctgatcttc
    13141 gtctcgccgc ccttcatcgg ctcctactcc tggatcatcc tgctcggccg caacggcctc
    13201 gtcaccacga ccctggcgga atacggcatc tcgctgccgt cgatctacgg tgctcagggc
    13261 atcatcttcg tcttcaccct gcattatttc cccttcgtct ttctgctggt gtcgtccggt
    13321 ctcaagtcga tcgaccagag catcgaggat gccgcccgca atctcggatc gagcgagctc
    13381 gcgaccttca ggaaggtgat cgccccgctg ctggcgccat cgatctcgac cggagcgctc
    13441 ctggtcttca tcgcctgctt caccgatctc ggcacgccga tcatcctcgg cgagcgcttt
    13501 cgcgtgctgc cggtcctgat ctatggcgag ttcgtcaacg atttcggcgg ccggccggtg
    13561 ctcgccagca ccctctccgt gctcctgctg ctgatcacga cctcggcgct tctgctgcag
    13621 cgctggtacg cctcgcgcaa cgccttcggc accgccgcgg tgcgcccgct gaccctgcag
    13681 gaacagccgt tcaaaaaacg cctgctggcg gcgctcttcg tctacacgct ggtcggcgtg
    13741 gccctgctgc cgctcgccaa catcatcgtc tcgtccttcc tgaaggccaa cggaccggtc
    13801 ctgctggcgg aattcaccct cgacaactac cgccgcatcc tcggcaccat cggcaggccg
    13861 ctctggaaca ccatcttctt caccaccacg gcaaccctgc tgtgcgctgt cttcggcacc
    13921 atgatcggct acatcatcgt ccgccggccg ggccgcatca gcggcttcct cgacaccatc
    13981 atcatgttct cgttcgcggt gccgggcatt gtcctggggg tcgggctggt ggtcacctat
    14041 caccaccagc ccttcgccat gaccggcacg gcgctgatcc tcatcctggc ctacttcatc
    14101 cgccgcctcc ccttctcggt ccgctcgtcc gtaggcatgc tgcagcagct ctcccgcgac
    14161 accgaggagg cgtcggtcaa tctcggcgcc gggccggcca tgaccttcct caagatcacg
    14221 gtgccgctcc tgtccgtcgc gatcctctcg ggcgtcctgc tcacctggtc gaacaccatc
    14281 cgcgagctga gcgcgaccct gatcctgcag tcggggcgga cggtgacggt cagcctcgag
    14341 atcttcaacg aggtggtcaa cggaaacttc ggcatcgcct cggcgctcgg caccgtcctc
    14401 atcctcctga ccttcctgcc tctctccgtg ctgttcacgg cgatgggcaa gcgagaggat
    14461 gcgctcgtgt gagggacggc cggaggcgct cgactggcac ccggcagcca tcgacgaccc
    14521 gtggcgagga gcacgattgg ccagcaggca acgcatcgga tcgggtcgtg gcagcgcgct
    14581 gaacgacgat atcgttctgg aggcggcctg gctctactac tacgagggca ggagccagca
    14641 cgagatcgcc ggcacgctcg gcgtctcgcg ctcgtcgatc gtcggctatc tgcagcaggc
    14701 acgcgagacc ggcatggtcg agatccggct cgatgccggg gtcttcagcc gccatcgcct
    14761 gtcgcacgct ttgtcgcaac gctacgggct ggccgagacc tatgtcgtgc cgggcgacac
    14821 gttctccctc gagcgggtcg ccgtcggcgc gggccggcgc ctgccggacc tgatcgatcc
    14881 cggcgacgtg ctcggcgtcg cctgggggca gacgatcttc aacctgtcac agaacatcgt
    14941 gccgcggcgg atcgaccggc tgacggtcat ccagatggtc ggctcgatgt cgtcacccta
    15001 cggcttcgat gcggagatct gctcgtcgca catcgccctg tcgctgggcg gccgctgcat
    15061 caacctgcac gccccggccg tgctctcgac cagtgcgatg gcggaggcgc tgcgccagga
    15121 gcccatcatc agggcgcagc tcgagcaggt ctcgcgcatc ggcaaggcgg tcttcgccgt
    15181 cggctcgtgc aacgagacca gccatatcgt ccaggcgggc atcgcctccg tcgacgacct
    15241 cgcccgctat cggcgccatg gcgccgtcgg cctggtctgc ggccgcttca tcgacgccgc
    15301 cggcaatcat atccatggcc acctcgacga gcggctgatc ggcgtgacgc ccgattgcct
    15361 gcgcggcttg cggatgggca tcctcgtctc atccggcgcc gacaagatcg aaggcatcca
    15421 cgcagccctg cgcggcggct acgcgaccca tctcgtcacc gacattccca ccgccgaagg
    15481 catcctcacc ctgagccgct gaggctggtt cacccgggcc gggaccgcct caaccgtcga
    15541 gtccctcggg cggttgccag cccgggggtg gcggctggcg gggggcctgg gggatgggga
    15601 cgtggaagtt ggcgtcggcg ccgttcatgt ccatgcgccg ctcggcgatg cgccagcctt
    15661 cgggcgtgcg ccgcagggaa tcgagatagc ggccgtagac ggtggcggtc cgaccgtccg
    15721 gccgctcgtg ccaggcgtgg acgcagctct ccgcccgcgc ggtgtcggcc gagtcgagcc
    15781 agacccgcac attcgccagg tgatgcgccg tgcggcgcca gcgccacatg cgcgccagcg
    15841 agcgcgccag cgcctcgcga ccgaccgcgg cgagcctggg ttcggcgccg taggtgacgc
    15901 ggcagtcggt cgtgaagagg gcggcgagcg cgtccagctc catccggtcc agatggcggc
    15961 aatagtcgac aagcaggtcc tcgatctggc ggcggtcgag cagctcccgc atcacctcag
    16021 ccatgttccc gcacccactg ggccagctcg gcgtgggtgg cgaaccaggc ccggcccttc
    16081 gcctgctcga tcagcgcctc gaggatccag atgcgcgagc ggtagccgat gacgaagggg
    16141 tgcatgacga gctggaagac gccgccctcg gccatcgccg cgtccagctc ccggcggaag
    16201 atcgcgagaa cgtcgtcggg tggcgtccag ggccgggtcg cgggctcgcg gttgaagagg
    16261 aagtagacgg catcgtcgcg cagccattcg accggcacct cgacgacccc ggtgggctcg
    16321 ccgtcgcaga ccagctcgta gcaggcctcg tcggccatga gcgagctgtc gtaggcgagg
    16381 cccagctcgg cgacgagcgg gatggtcgag gggctgagat cccagttggc ggcgcgaaac
    16441 ccgacggggg gcattccggt cagcccttcc agggtgtccc gggcgcgcag catcagctcg
    16501 cgctcctcgg cttccccgag cagcgaggtg ttctcgtgga tccagccatg catggcgacc
    16561 tcgtgcccgg cgccgacgat gcgccggggc tcgtccgggt cgatgaggcc ggcgaccgcc
    16621 ggcacgaaga acgaggccct gacctcgagc cgggcgagca cgtccaggat gcgcggcacg
    16681 cccacgcggc ggccgaactc gccccaggcg agccggccga tggcgctccc gccccggccg
    16741 agctcgaagg tctcgtgatc gcagtcgaag gacagcgcca cggcgcagat ggcgccgccc
    16801 ggccattgcc gcggcgccag cgcccggccg gcacgcacgc gcccgacgcg gcggcgccat
    16861 tcgctctcgg gcatctgcca gggctgcggg aattccgcca agggtgtcgc ccgtcctgct
    16921 gttcgtcggg tccgccgagc ccgtcgcggc gccgcggtta tgtatttgac agccccgtgc
    16981 cgagccttgc aaatgccaaa acagcccgga ccggcgcatg ccgcacaaat gttcgcaaaa
    17041 aagggagacc gctcagatgg ctcgaatcga cctcctcgca cgcgcatccg ccatcgtcgc
    17101 cgccaccggc ctcatggtcg gcgccttcgg cgccacggcc caggaatcga tcacttggcg
    17161 ggtgcagacc ggctggctgc ccggcaacac cctctacgac agcgtggtcg agctggccga
    17221 gcggatcgac ctgcagagcg aagggcggct caagatcgag gtcctgccgg ccggtgcggt
    17281 ggtcgccctc aaccagacga tcgattcggt caaggccggc atcatcgacg gccacatctc
    17341 ctatgccgcg gtctatgccg ggctcgaccc gggcttcgcg cccctgggcg acatcccggg
    17401 cgcctatgac gaccggttcc agaccatcga gttcatgtac tacgaaggcg ggctcgagct
    17461 cttgcgcgag gcctatgccc agttcggcct ccacacgatc ggtgtcggca ccggctcctg
    17521 ggaggccgtg ccttcgcggg tgcccctcga gagctgggac gacttcgagg gcgtgaagct
    17581 gcgcaccccg cccggcatcg gctcgctcat ctgggagcgg ctgggtgccg tgccgatcgt
    17641 catgccgcag accgaggtct tctcggcgct cgagaaaggc gtcatcgacg ccgccgatga
    17701 cggctcgctc tcctacaact tccaggtcgg cgactacgac atcgtcgact tcaccctggt
    17761 caacagcccg cattccaacg gtgtctggga cttcacggtc aatgccgacc gctgggcgga
    17821 gctgccgaac agcatcaagt cgatgctcga gatcaacatg cgcgagatca tgctgcgcaa
    17881 cgtcatgaac gcgctccgcc aggaccagga ggtgctggcc aaggccgacg agatcggcct
    17941 gacgctcttc gagctgacgc cggaggatcg cgccaagtat cgcgccatcg cggtcgaggt
    18001 ctgggacgag tgggcaacga agagcgagct ctcgaagaag gccgtcgacg cgcacaaggc
    18061 cttcttgacc aagaagggcc tgctctaggc ccgccaccct ccgcagccgc cgggacatcc
    18121 tcccggcggc tgcgcttcgc tttcgcgagc ggtgcccgcc gatgatcaca cgcttcaacg
    18181 agcagctcgg cctcgccctc tcctacgtct acgccctggt cgccgtcctg accttcggcg
    18241 aggtctgcgc ccgctacttc ttcaactccc cgacccagtg gaccatcgag gtcgtcgtcc
    18301 tcctggcggc gatgcactac ctgatcgccg ggccgcaggc ctatgcgagc gaggggcata
    18361 tccgcatcac ggtgctctac gaccgcctgc ccttgccggc gcgacgggcg ctgaccgtgc
    18421 tcgagcggct ggtcgtcgcc acgaccgcag gcctcgtcgg ctggtgggcg ctcggccagg
    18481 cgcagtccgc gctggccatc ggcgagcgca ccggcagcaa cctcaacacc tactcgccga
    18541 ccatcctcaa ggtcgtgctc gccattgccc tcggcctcct cgccctgcag gccctggccc
    18601 acctcgtgcg ggaccgggga cgggcgcatg gcggctgagg tcgcgaccta tctcgtcctc
    18661 gggctgctct tcttcttcat agccatcggc accccgctgg cgatcgccac catggcgacg
    18721 gcgatcatcg tcggctggtc gctcttcggc ttcaactcga tcctgctgat cgccaaccgc
    18781 atctacgacg cctcgaccaa ctacgtgctg atcgcggtgc cgctcttcat cctcatgggc
    18841 gtgctgatgg agcggggcgg gatcgcgcgg cggctcttca ccgtcctgca catctggtcc
    18901 gcccggctgc cgggcggcat ggccgtgggc tgcgtcttcg ccggtgcgat catggccgcc
    18961 atggtcggca tcgtcggcgc cgagatcatc acgctcggcc tcgtcgccct cccggccatg
    19021 ctggcccgcg gctacgacaa gcagctcacg ctcggcgtca tctgcgccag cggctcgctc
    19081 ggtgccatga tcccgccgag cctcgtgctc gtcttctacg gcctgatcgc cggcgaatcg
    19141 atcgcgcgcc tgttcgcggc agccctcgtg ccgggtctgg tcctggcgct gagctatgcg
    19201 atctacatcg tggtcagggc caagacccgg cctcacctcg ccccgccgcc gtcgcaggac
    19261 gagctcgaga tgccgatcgc cgagaagctg gcgcttggcc gggccctctt cctgccggcg
    19321 ctgatcatca ccggcgtgct gggctcgatc tatttcggca tcgccgcccc gaccgaggcc
    19381 gcggccatcg gcgtcgccgg tgccttgctg gcggtgatcg tcagcggcga attccgcctg
    19441 gccgacctgc accacaccgt cgtccagacc ggcaaggcga tcggcccggt gatctgggtc
    19501 ttcttcggcg ccaactcgct gatcagcatc tactcgctct cgggcggcgt gaagtacctg
    19561 gccggcctga tcgccgccgt gcccctgccg ccgctcggcg tcctgctgct gatcgtcgcc
    19621 atcctcttcc tcctcggcat ggtcatggac tggatcggca tcgccatcct gaccatgccg
    19681 gtcttcctgc cggtcgtcgg agcactcggc tacgacccga tctggttcgg cgtcatcttc
    19741 tgcatcagca tgcagatcgc ctacctgacc ccgcccatcg gcgcctcggc cttctacctg
    19801 aagagcgtgg ccccgcccga cgtgacgctc ggcgagatct tcatgtcgat ctggcccttc
    19861 gtcgggctgc agctcgtcgt gctcgccctg gtcatcgcct ttcccgcgct ggcgctctgg
    19921 ctgccggccc agatgggctg agccctatct gatctcgagc aggccgccgt ccaccgggat
    19981 gatctggccg gtcatccagg cggagcgctc ggaggcgaga aacagcacca cctcggcgat
    20041 ctcggcgggg ctgccccagc gcggcagcgg gtagcggccg atcacctgcg cgagcagcgc
    20101 ctcgaaatgc gcctcgtccg tggccctggc gcggaactgc gattccgaga gcggggtgcg
    20161 caccgcgccc ggcgccacgg cgttgacccg gacaccccgg ccggcgaagt cggcggcgag
    20221 ctggcgggtc agcgccacga cggccccctt gctcgccgag taggcggccg tgcccttcat
    20281 gccgacgagg cccacggtcg aggccgtgaa gaccagtgtt cccgccccgc tccgggccag
    20341 atgcggcagg gcggcccgcg cggtgaggaa gctgcccgtg gcgttcacgg cgagcgccct
    20401 gttccattgg gcgagcgaga gggcgagaat gtcgccctcc accatgatcc cggcattggc
    20461 ggcgacgctg tcgagccgcc cggtccaggc cagcgccgca tccaccatgg cggcgaccga
    20521 ggcctcgtcg gtgatgtcga ccgcagaggc cttcgccgca tggccagcct cgccgagcgc
    20581 catcgccacc cgctcggccg cagcaccatc gaggtcggcg caaaggaccg aagcaccttc
    20641 gagcgcgaac cgctcggcaa tgccccggcc gatgccggaa gcggcgccgg tgacgatcgc
    20701 ggtccgcccg gcaagcgctc cctcagccaa ggcgggccac cgcgtcgacc ttcaccgtga
    20761 ccccggcctg ggcggtcggg atgaacggga cctcggccag ctcgaccgca caaaacggca
    20821 gaggcctctc cttgatgagc cgcgccagca ggtcccgctc gctggctgcg gtatattgca
    20881 gccggagatg aacgacccgc tcgaggctcg agcccgacgc gcggaggatc gcctcgagat
    20941 tgctcatggc ctgggcgagc tggacagcgg agccgccggg cacgggtgcc aggctgccgg
    21001 aggccggatc gctgcccacc tgtgcggtga cccagacccc gccctcgtgg gctcgcgcat
    21061 gtggaaaggg cgcgcagggc gccgggccat cggcggcgat gatggcggca tccggcccgc
    21121 tgccgggtgg tgctccggtc gccgccaccg ccccgacctc gaccagcccg ccatgcgcga
    21181 ggccggtggc agccgagacg cagcgggccg gccggggcat ggcggcgaag cccgcgagaa
    21241 acgccgcctg cagcgcgcca taggcctcgg gcccgctccg gtcgacgcgg atctcggcgc
    21301 gcaggacatg gtccagcgag ccgcccgccg cccgaagcct cgcctcgagc cgcgccaggc
    21361 tcgcccggaa ctgggcgccg aagccgtcgg gcatgcggcc ctgcgcgtcg agtggcggct
    21421 ccggcgggaa gaggcgagct tcaccgatgc cggcggttgc ctcggcgagc ctctcccagc
    21481 aagacaactc gatcacgttc tgcacgacca cccgcccttc ctcagcttcc cgccttcgcc
    21541 cgggtcgcat ggaggtgcgg gtagagattg cgcaccgcgt cctcgtcgag ctccgccttg
    21601 aactcgtgcc gctcaccgat cgccagcgcc cgcttcgccg ccgggcgagc accgatctcg
    21661 tccaccagcc ggccgagatg cgggaactcg gccatcgcct cgtcgccgag catgaagggg
    21721 atgcgcgtgg cccagcccca gaccgacatg tcgacgatgg tgtagctgtc gcccagcatg
    21781 tagggccgat cggccagccg gtcgtcgacg atcttccagt gccgccgcgc ctcgaaggcg
    21841 tagcgctccc gcggataggg atcggaaacc ggggcgaaat ggcggaaatg cacagcctgc
    21901 cccgaatagg ggccgacgcc gctggcgacg aacagcagcc aggacaggag ctcgcctcgg
    21961 gccaccggcg tctgcgccgg caggaactga ccggtcttct cggcgaggta gaggaggatg
    22021 gcgccgctgt cgaacaccac gacctcgccg tcgacgatgg ccggcacctt ggcattggga
    22081 ttgatcgcca gatactcggg ccggtgctgg tcgccccgcc gcgtgtcgat cggcacggcc
    22141 tcgtagtcga gcccggcctc ttccaggaag agcgccacct tgatgggatt cggagccgta
    22201 ttgtagtaga atttcagcat cgccgcgggc cttcctcgtt gctgcttcgg ctgtgggggg
    22261 aggtttggca cagaatgcgc ccgtcgacac gcgccgcgtg tagcgcggtc tgtcgcttgg
    22321 actggcctgc cgccatttcc gcattgacag aggcacggcg cggcgacctg ccgaaagggt
    22381 gagtggggcc ggccgtgatg gcgccctcat caggggaggg agcaggtgtt gcgtgacgag
    22441 cggttcgagg ttgagccttt caccggatca ttgggcgccg ccattcgcgg catcgacctc
    22501 gtggccgacg accacgccat gatcgacgag gcgcggcgct tcgggccgtt cggcggcaac
    22561 ccggtccaca cgccggtcga gggctacgag gacatcatgc gcttcgcccg cgagcccgac
    22621 gacagcggca aggtgatcgg cgaggaaggg cacatggacc tcgcctggct ggctcggccg
    22681 cccggcatcg tcatgctcgt cggcgaggag gtcccgcccg tcggcggcga cacctgcttc
    22741 accagcctcg aggaggcctg ccagtccctc tcgtcccgca tggtggcgat gggggccgag
    22801 cttgccggca tccacagcgg ccgcggcgtc ttcgccatca acgccgcctc gcacgcctcg
    22861 gcctgaagga cgagcacgag gccgtggaga acatgcgtcc gcatcgaggg tgccatcccg
    22921 gtcggcgcat tggccgaacg caggaacatc gccgaggcaa cggccctctt cctcgtctcc
    22981 gaggaagcct cctacgtcac cggcgccacg ctctacgtca atggcggctt ctagatgggc
    23041 cgaccaggcg cccgaccttt tcgccggccg ccagcgtctc cagcccgtcg agcggcccct
    23101 ccaccagggc gaggacgggc acgcgcccat cgaccgcgag acccgccgag ctgatccagc
    23161 cgacattctc cccccggcgc agcacgggct cgagcggctc gatcgccgcg gcctcgacac
    23221 gcgccgcaac cagccgcgca cgcggtgcgc tatcgccttc gcccgccacg gccgcccggc
    23281 cgacgaagtc gcgacccagc tccagcgacc cgaccagccc cgccgccaag gcaccgtgaa
    23341 caggagtcgc gtcgatcccg gcgcgcggcc ggccggccag gatgcgcagc gtctcgtcga
    23401 ccaggtgccc ggccggcctg gcaccttcgc gccgcagcgc ctcgtcgaca tgcaccgcca
    23461 tgtcggcggg gaccagaaga cgccaatgcg cctccgcgag gtgggcaatc acgccctggc
    23521 ccaacgcgag cccggcgccg cgtgcccgac ccggcatcgg caacggcgca tcgtcggcga
    23581 cccgcgccag cagcgcctcg gcggccgggc ccacgagatc gagcgccgcc cactgcgccc
    23641 cggcatcgac cacggccacc cgctccgtcg gcagtggctg caccgcccgg cgcagccagc
    23701 cgagcccgtc cggatcggcg agcagcagcc agctcgccgc gtcgagccgc accgcccagg
    23761 ggcaggccac gacgccgccc cgcgcgtcga gcatcggagc cagcactgcc gcatcgtcgc
    23821 gatcgacgcc cacggccccg ctgagccgcc ggaccatcgc cccggcgtcc ggcccggcga
    23881 gcagcagctt cgcgtcggcc gagcgctcga tgcggaccac ggccccgcgc gcggcggcga
    23941 gctcggccgc cgcgctgcca ttgccagtga accacagagg ccgctcgtgc cccgccacgg
    24001 cctcgaggac cgccccgtcc gcgacatgcc gggcgtgcag cggcgagc
//
LOCUS       DAOTFI010000036        23896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2485_length_23896_cov_7.066482, whole genome shotgun sequence.
ACCESSION   DAOTFI010000036 DAOTFI010000000
VERSION     DAOTFI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 23896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23896
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2485_length_23896_cov_7.066482"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="P5558_05350"
     CDS             complement(<1..787)
                     /locus_tag="P5558_05350"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRY23766.1"
                     /translation="MSETSIQAFARADGGKGIRNLVVVAYLVECAHHVARAVTVPFEG
                     DGVHLIGFAGCYPNGYAEEMMEALTTHPNVGAVLLVSLGCEQFNRQKLMKRVEASGRP
                     VELLVIQKAGGTRSTVEAGRAWVSEQVAALAKAPRVPMGLDELIVGTICGGSDGTSGL
                     TANPAIGKAADILVDAGARVMFEELGELFGCEAHMAARAATPELGRAIMDAMDKSRRV
                     YGMLDQSSFGGGNITGGLSTIEEKSIGAYTKSGTRPIVGMLKPG"
     gene            complement(784..1086)
                     /locus_tag="P5558_05355"
     CDS             complement(784..1086)
                     /locus_tag="P5558_05355"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRY23767.1"
                     /translation="MTGDRALLHLAPDDNVAVALRALVPGETVEIDGLAVTVRDAIPF
                     AHKIALAELPQGATVTKYGVAIGSTTAPIAAGAHVHVHNLKSDYIVNREDFFEAAS"
     gene            complement(1148..2821)
                     /locus_tag="P5558_05360"
     CDS             complement(1148..2821)
                     /locus_tag="P5558_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRY23768.1"
                     /translation="MTVELDHFALWTGLFGGMALFLYGMDVLTVSLKRAAGEHLKDLL
                     GRVTRNRFLGAGVGALVTGIVNSSTVTTVILVGFISAGLMSMAQSVAVIMGANIGSTV
                     TAQILAFNVSRFALPILTLGFLVAFVPKGERTREFGRMLLGAGLVFYGIAVMSEAMAP
                     LRAYPPFIDFIKTLSHPLPAALAGMTFTALVHSSAATTGVVIVLAGQGVMSLETAIAV
                     ALGANVGTCATAGLAAIGKPREAVRAALVHVLFNVAGVAIWIGFIPELAEIVRAVSPA
                     ATNLTGSARAAAEAPRQVANAHTIFNIVNTLLFIGFTTQIARLVEWLVPDRPLGADEP
                     LAPRFLDDNLLSTPAMALDSARREIGRMGDYVGAMLERALPAALSGSRQALYAIEAMD
                     KPVDGLHRALIAYLSRISATPLTPDQAPVLMHLVAMTNDLEHLADRIATDMTTSARKR
                     IDERVVIAPDAASRITAYHAAVVKALRDAVTAVMTDDHELATTVRNMKHGVTDMASQI
                     SRERFGHVPKGAQNAIRGYVREVELLQILDGIFKIARRIARTELVPEPA"
     gene            complement(2818..3312)
                     /locus_tag="P5558_05365"
     CDS             complement(2818..3312)
                     /locus_tag="P5558_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY23769.1"
                     /translation="MTAPVLCAVDFSPDAEAALLWSCAQAELAQAPLLLLHVIHDPAA
                     SPGFYQRPDDGWLRPLADVAEQMLGEFLDRLRQEHPDQGRLKTLETRLVPGLPAGRIV
                     EVASEAGAVLVVVGSRGRTGLPHILLGSVAERVVQIAPMPVVVVKRAATVAADADQAD
                     GGRK"
     gene            complement(3309..4883)
                     /locus_tag="P5558_05370"
     CDS             complement(3309..4883)
                     /locus_tag="P5558_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRY23770.1"
                     /translation="MAAGVPNIRKLESALLVNRAELFRFGTAVLFVIVIMIFAVAQGA
                     SGPHGLLLVAAAMIGGYMALNIGANDVANNVGPAVGSHAITLTGAIVIAASFEAGGAL
                     IAGGDVVGTIKSGIIDPALIPDATTFIWLMTAALLAAALWLNLATALGAPVSTTHSIV
                     GGVLGAGIAAGGFGIADWAEVGRIVASWVISPVLGGLIAAGFLYIIKRRITYQSDLMA
                     AAERTVPMLIALMAWSFTTYLLLKGLKQLWSVSFLTAALIGLVLAGVAWFVMRRHVAS
                     SAPRVPNTKGGVNELFTVPLIFAAALLSFAHGANDVANAVGPLAAINDAVSSGGVASK
                     AHIPIWVMLVGAIGIALGLALFGPKLIRTVGSEITELDRARAFCVALSAAITVIVASQ
                     LGLPVSSTHIAVGGVFGVGFLREYLKVSYARMIEEIREHHADADAAAVEAFLARFEAA
                     KVSERQLMLRQMKGQSGGATLRKKERKGLRNINRIELVKRSLLLRIAAAWLITVPATA
                     IVGAMIFFMLRGAMLP"
     gene            5091..6794
                     /locus_tag="P5558_05375"
     CDS             5091..6794
                     /locus_tag="P5558_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRY23771.1"
                     /translation="MGDDGRTRGLANGLTNYGDPAFSAYLRRSFASSMGYSRDLLARP
                     VVGIAHSASGFNNCHRLVPELIEAVKRGVLAAGALPLDFPTISLGEGFLTPTSLKFRN
                     LMSMDVEEMIRAQPMDAVVLIGGCDKTVPAQVMGAISAGRPAIQLVTGPMATAMHRGE
                     RLGACTDCRRFWARFRAGEIDEAEIDRVERRLASTAGTCAVMGTASTMASLVEAMGLM
                     LPMGASIPAVDADRLRLAEATGAAAVALIGSGRPMAELVTQESLENALRVLLAIGGST
                     NAVLHMAAIAGRAGLELDLRRFNELSETTPVIVDLKPTGTHYMQDLHAAGGLPAVMRE
                     LGDLLHLGVPTVTGETLGERLAAADPWVDRTVIRPIDDPIHPKGGLVALFGSLAPKGA
                     VLKRSAADPRLFEHEGRAVVFDGLEDLAARIDDPDLDVTADALLVLKNAGPTSPAAMP
                     EAGYLPIPKKLARAGVKDMLRLSDARMSGTAYGTIVLHIAPDAAAGGPLALVETGDRI
                     SLSVERCRLDLLVEPDELARRRQALPPPPPLPARGHDRLYQEQILQADEGCDFGFLKG
                     S"
     gene            6980..7165
                     /locus_tag="P5558_05380"
     CDS             6980..7165
                     /locus_tag="P5558_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HRY23772.1"
                     /translation="MGIEVGGLIGLLVLIADVWAIVNVFSSGASVGSKVVWTVVILVL
                     PVVGLLAWLLFGPRAAG"
     gene            7347..8771
                     /locus_tag="P5558_05385"
     CDS             7347..8771
                     /locus_tag="P5558_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRY23773.1"
                     /translation="MLDGFSAIDLARFQFAFTISAHIVFPAFTIGLASFLAVLNGLWL
                     WRRDEAYLRVFDYWKTIFAVVFGMGVVSGIVMSYQFGTNWSVFADRTGPILGPLMGYE
                     VLSAFFLEAGFLGIMLFGRERVGDRLHMAATVIVALGTLFSAFWILSVNSWMQTPQGY
                     SINEVGQFVPEDWWLIVFNPSFPYRLVHMVLAAYLTTAFVVGGVGAWHLLRDRENRAA
                     SIMFSMAMWMALIVTPIQILAGDQHGLNTLAHQPAKIAAMEGHFESFPEGRAPLVLLG
                     LPDDAAGETRYKLEIPLLGSLILTHDLDTPIQGLDAFPADERPPAALIFWTFRIMVAV
                     GFAMLGIGLWSAWARWQGRLDDARWLHRAAVLMAPSGFLAVLCGWITTEVGRQPYTVY
                     GLLRTVESAAPVQAAAVSASLTAFIVVYFLVFGSGTFYVMRLMGREPRSSIGIEQIGP
                     TRAAGITPVAARPTQWHASTGGQP"
     gene            8768..9775
                     /gene="cydB"
                     /locus_tag="P5558_05390"
     CDS             8768..9775
                     /gene="cydB"
                     /locus_tag="P5558_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRY23774.1"
                     /translation="MSIDLPFIWVGLIAFAVLAYVLLDGFDLGIGILFPFLADREERH
                     LAMNTIAPVWDGNETWLVLGGGGLFAVFPLAYAIVMPALYPLIIAMLLGLIFRGVAFE
                     FVHRTRRMQGFWELGFWGGSLVAAIAQGMALGALVQGIAVEGRAYAGGSWDWLTPFSA
                     LTALALVVGYALLGATWLILKTEGATLARARRFAMPLGVALIALIGAVSLATPFLRPE
                     YLERWFGWPSGAWSAVVPILLALAVWQLWTGLRHQEDHRRPFLATVAIFILSYAGLGV
                     SFYPNLVPPALTIAEAAAPTSSLLFLLVGAALLIPMILAYTAYSYWVFRGKVGPADGY
                     H"
     gene            complement(9864..10229)
                     /locus_tag="P5558_05395"
     CDS             complement(9864..10229)
                     /locus_tag="P5558_05395"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23775.1"
                     /translation="MLAALQPIAAHLRQMRRQLVFWGAAALLGGLAIIFFAMAFYMSL
                     ARQLGEPLAAGVLGTVLALGAIILFLVGRRRSVRRHYPSGSPVPPLTTPTPPRPMEAL
                     MVAFVLGVIDEQFRRRPRR"
     gene            complement(10238..10558)
                     /locus_tag="P5558_05400"
     CDS             complement(10238..10558)
                     /locus_tag="P5558_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23776.1"
                     /translation="MAARDTDGDKDLAALREDLARLREDIASIKSTIAGIGKRSAQEA
                     KEAGSAKLGELRDELEQLGDNMYARGHDALAGVERTVHDRPLTSLLAAFGVGLILSKF
                     LDRR"
     gene            10830..11009
                     /locus_tag="P5558_05405"
     CDS             10830..11009
                     /locus_tag="P5558_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HRY23777.1"
                     /translation="MLYWALVFFVVAIIAAVFGFGGIASASAGIAQLLFFIFLALLVI
                     SLVVYVIRGRSPPVT"
     gene            11246..12292
                     /locus_tag="P5558_05410"
     CDS             11246..12292
                     /locus_tag="P5558_05410"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRY23778.1"
                     /translation="MRKILMSSVASMALLGVAAGFGGPLLAQTDAPAATAQDQNAQNG
                     DTQEQGNVPASSQGDATQSAETQGGDAVGAAGATGADQPLASAPPEEMRYRSIQDAEA
                     GSITLPNEMTTEEIVGANVYDANDESIGEVVDLLVTEDGQVSQVLVDVGGFLGMGEHR
                     VALDLDEIEFVDADGVLRASVDVTEEELEAMPAFERAEDNYWVGSAGPVGSPVMPDTT
                     AEQTRTAATEAPAVDDTEYRSWDQAGEADMMMGDMSAKDVLGATVYGLDGDGIGEVAD
                     LLIGPDNNIDRLIVDVGGFLGMGVHTVALPADEMQIAAVDGEQRITVSATKEELEAMP
                     SYERDADGKWLVNE"
     gene            12359..12565
                     /locus_tag="P5558_05415"
     CDS             12359..12565
                     /locus_tag="P5558_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRY23779.1"
                     /translation="MNWDIIKGNWKIFRGKAQEQWGKLTDDDLDVAEGNYDQFVGRIQ
                     KTYGISRDEAEKQVKDWQSRQKPL"
     gene            complement(12669..13304)
                     /locus_tag="P5558_05420"
     CDS             complement(12669..13304)
                     /locus_tag="P5558_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY23780.1"
                     /translation="MTVFRDDVVALVPHLRAFARSLAAGDAALADDLVQDTMVNALQA
                     QHQFQPGTNLRAWLFRILRNRFLSIKARKYQTSEVYDEHLETRWSVPAYQESMIEIDA
                     LKRAFGALSATHREVLIYAVVEELPYEEVAEICGCAVGTVKSRVNRARAILKKMLLED
                     ELPVDEARLARNLPDRMLERLVPDADEPGAHLHRDDGAVDASWARRPMGRA"
     gene            complement(13337..15133)
                     /locus_tag="P5558_05425"
     CDS             complement(13337..15133)
                     /locus_tag="P5558_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY23781.1"
                     /translation="MIAGDLDGDAPTGRGRWRQPASLKGRMALLLGLVLIPPLLYSVY
                     QTGSAYLAEAHEQERKAASLLDIVVAYQREVLDGTRNLLDTLVRDSSLQNDEASCNAA
                     LAAVAKQNPIYLSLAILDATGEIRCSSRPESVGVSLADRAYFRELAGGRDFVVSERLI
                     SRFNQRETIVVAEAVRNGVGGALESVVAVGLDLAVFTRAMRGIDLPDGTAVHIFDAEG
                     MIVGGELADAGEKPDLAPAMADLRALATSPREVVELTSRDGVQRLFVSAPVAEGALFA
                     VVGFAAPPRWDWLDSKLLIGILSPTVMLALAVVAIWIASDYLVNRHIRTLVAATRAYG
                     RGRGAIRPDTKGAPEELKELAGSFAEMAARIEHREQELRSSLEQKDMMLREIHHRVKN
                     NLQIVTSLLRLRARSAASVEARRVMDEAQVRIRALALVHRHLYEQPAAESLRVDTFVE
                     DLLDFLAALGTGDDRGIAFRTNLAPVEIASDQAIALALFVTEAVGNAQLHGFPGAHHA
                     PEVRIDFTVEGDCATLVVADNGVGAGQGREDRAQALEGMGLRLMRMLAGQLGGECVIE
                     HQAGTTVRLTFPLHDFDVFESSSEGTFEPADG"
     gene            complement(15130..15951)
                     /locus_tag="P5558_05430"
     CDS             complement(15130..15951)
                     /locus_tag="P5558_05430"
                     /inference="COORDINATES: protein motif:HMM:NF006623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY23782.1"
                     /translation="MTGGQPGQEIARVLPFLRRYARAVTGDQARGDRDVAAALTGLAD
                     RLAEERLHDPRLALYRALHDTWNGSRLPAAVAEEPSDSDLLEGTLALETRLHELAPPF
                     RQALLLATLEEFDRSEVAQIMAVGEAEVDALLDEAKAELRRQPPSRVLVVEDEPVIAL
                     DIATSLEGNGHTVVGIATTHRQAVALSRQHRPDLILADIRLADESSGLEAVHEILREA
                     ELPVVFVTAYPERLLTGTRPEPTFLVTKPFDADTLDITISQALASARRRRREQPA"
     gene            complement(15953..16243)
                     /locus_tag="P5558_05435"
     CDS             complement(15953..16243)
                     /locus_tag="P5558_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23783.1"
                     /translation="MGKGAKEPDGAAGDQARGVARKRRSAQDAGFDRWLGKQLHAIYD
                     PVLDEELPERLRLLIEQFEPRDESASAAEDREDEEKMNGNRPQKRGGPVGGA"
     gene            complement(16368..17120)
                     /locus_tag="P5558_05440"
     CDS             complement(16368..17120)
                     /locus_tag="P5558_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY23784.1"
                     /translation="MARLEPLSTTTPCQNCALRALPIFRCLSGAELAFVEKFKIGELE
                     VQAGSDILLAGTHSAHLFTIFGGWCFRYDLMEDGRRQIHSLLLPGDFIGLQTEMLGTM
                     QHSVQSLTDVRLCVFSRERFWTIYESFPELAFDLTWAAACGERTVEVALSNVGQRPSF
                     ERLAALVVHLYRQLDGLRLVKDGTILWPLTQQHVADLLGLSTVHVSRVLRRLVDSGLV
                     RLDGRRLTVVDLPALAEVAAVDPEKPLRRPLI"
     gene            17273..18010
                     /locus_tag="P5558_05445"
     CDS             17273..18010
                     /locus_tag="P5558_05445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY23785.1"
                     /translation="MRKPPRARHARVATRHRTADDRSPASLELSELRHRTQNLLQTIQ
                     GLLGLEARRAEHAETQERLHDLALRVDLLAGLHQRLNALGPGGDVDAGALIRESAEAL
                     ILPNERVIALECEVEKLPLDGQRAQSLALLVHEALTNVTRHAFQEAQTGRVVVRARQT
                     RDGGFLVEVADNGRGMRPPPGSGGLGLRLMRTLALRLGGELRLEDGAPGLKLTVLVPP
                     PEPVAADTAGPSAARVAAPPRKCAAAV"
     gene            complement(18076..18804)
                     /locus_tag="P5558_05450"
     CDS             complement(18076..18804)
                     /locus_tag="P5558_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23786.1"
                     /translation="MPEPVRDGLRLDGQRVLVTGAAAGIGEATARLLASLGATVLLAD
                     RDDTSAIANELRGSAFRVDLGSAEALDELVAAAGPVDALVANAAICPFDDWQEAGWDA
                     VFDEVIRVDLLAPIRLARALLPGMAARGGGRMVFVGSLAGKTGGLIASPHYVAAKGGL
                     HAFVRWLARRAAPRGVIVNGVAPASIRTPLMDGRPVSTSAIPVGRLGEPLEVAWPIAF
                     LSSPAASYVCGTVLDVNGGVRMDG"
     gene            complement(18797..19834)
                     /locus_tag="P5558_05455"
     CDS             complement(18797..19834)
                     /locus_tag="P5558_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23787.1"
                     /translation="MKQQSGLLRFLSVWHLLVLLVALVVLFTVIKPDTFPTAFTFKSM
                     ASSRSINAMMALAVMIPLACNQFDLSAASIVGIGQVLANGLQTQQGLSWPAACAIVLA
                     IGALVGLVNGLLVTRARINSFIATLGTGTLLLGVNQWYTGGRQVVGPLPDGFTGLAAV
                     VPGMAVPLPLIYALAIAFLLWIVFDHRPLGRFLYVIGDNPRAAVLSGIPADRYVVLAF
                     VASGVIAAIAGIVLQAQLRVGQSTVGQELLLPAFTGALLGATAIRPGRPNPWGTILAV
                     AVLAVAVAGLSQLGAPFYVEPIFNGAMLILAVGLAAWMTRRREQRLATAEAERRRATG
                     PPPEAGEAGRA"
     gene            complement(19831..21324)
                     /locus_tag="P5558_05460"
     CDS             complement(19831..21324)
                     /locus_tag="P5558_05460"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY23788.1"
                     /translation="MQPPLLAAEALTRRFGGTLALDAVDLALEAGEIRALLGENGAGK
                     STLIKILAGVHARDAGRITMDGVAVEPMAGKLPIAFVHQELGLVDGLSVAENIALYRG
                     FPLRRGLIDWPRVQREAREVLELMAADLDPDMKVGELPAAERALIGIARGLASKARLL
                     VLDEPTAALPASDVERLHAALRRLKREGLGILYVTHRLDEVAALADSVTVLRDGRHVA
                     TRPVAGTTSAMLAELIVGRSLDELFQPPPGSGDAAPLLELDRLELDGVGPVSMTLHEG
                     EILALVGLRGAGHHELGRFVFGAVRGRGGVARLAGRPWRPASPAAATAGGVGFVSSRR
                     VEESLAPGLAVRENLFMNPPLTAGRARINDRSERSRAASAMQRLAVRPAEPEQPVLNL
                     SGGNQQKVVIARWVETGMRLLVLEEPTFGVDVGAKAEIYRILDRLVGEGRAVLLISSD
                     FEEVAGIAHRALVLDRGRITAEVPRERLSVPLLTRLAGGEALTGAAA"
     gene            complement(21350..22441)
                     /locus_tag="P5558_05465"
     CDS             complement(21350..22441)
                     /locus_tag="P5558_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY23789.1"
                     /translation="MKLPIAALGAVLVLGTSSFALGQSVLDEAKKTVAERSGPQTVWD
                     GPTEGPKPEPGKKIAYLSTDEQNDASREWGQAVKEAGELVGWEVTIIDGRGSPKTWIE
                     GFNQAIALQVDGVVTTADIASLQEPVQAAKEQGIVVLGIHGLAFPGPDEAMGVFYNIQ
                     QDPRDIGRAQADWIIADSDGKGRVVVTTHCEYAIACAKAEATRDRILECEECEMLEFS
                     NTPIAEAAQRQPQLVAAWVQQYGTPLYITSVADYTADFQVPALRAGGVDPADVILVSA
                     DGNRSAYERIRAGGEYQRVTASEPYELQGFQAIDEFNRAFHGEPPSGWVQKPYLVTAD
                     NIDAEGGDQNGFRPSMDYKGAYKKIWGIE"
     gene            22598..23323
                     /gene="nanR"
                     /locus_tag="P5558_05470"
     CDS             22598..23323
                     /gene="nanR"
                     /locus_tag="P5558_05470"
                     /inference="COORDINATES: protein motif:HMM:NF003011.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HRY23790.1"
                     /translation="MPQAPIRRRKVHEEVAERIEHMIREGDLQPGDFLPSEREFMAAF
                     GVGRNAVREAMLALQRMGLVAVSSGERSRVVRPTAEAMVAELAGAARLLLADDDGMRQ
                     FQDARSLFEGAIARRAARLATPADLARLEEILDRNRKAIGDHRAFMAIDVEFHMALAD
                     ICRNPLFTAVHGAVVEWLIDQRAISGKAATAGEAAYAAHVRILEAIAAHDGAAAQRAM
                     EDHLAAVAELYWQIRATNDTRGA"
     gene            23327..>23896
                     /locus_tag="P5558_05475"
     CDS             23327..>23896
                     /locus_tag="P5558_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRY23791.1"
                     /translation="MAEYPGSEHERRVPAADLADGVARIFEAAGMGPADAALLADSLV
                     HADLRGIHSHGTLRVPDYVKKLTTDGVDPRAVPVVERRAGAAIVIDGANAMGQIAMDL
                     AMRTVIEAAGEHGIAMAAVGRSNHCGAMDYWAMRALPHDMIGIAGTNALPTMAPWGGA
                     EKLVGMNPLAIAVPAAEEPAIVLDAAFGMT"
BASE COUNT         3627 a         8391 c         8290 g         3588 t
ORIGIN      
        1 cgcccggctt gagcatgccg acgatcggcc gggtgccgct cttggtgtag gcgccgatcg
       61 acttctcctc gatcgtgctc agcccgcccg tgatgttgcc gccgccgaag ctcgactggt
      121 ccagcatgcc gtagacccgg cggctcttgt ccatggcgtc catgatcgcc cggccgagct
      181 cgggtgtcgc ggcacgggcc gccatgtgcg cctcgcagcc gaagagctcg cccagctcct
      241 cgaacatcac ccgcgccccc gcgtccacca ggatgtccgc cgccttgccg atggccgggt
      301 tggcggtgag cccgctggtg ccgtccgagc cgccgcagat cgtgccgacg atcagctcgt
      361 cgagccccat gggcaccctg ggcgccttcg ccagcgccgc cacctgctcg ctgacccagg
      421 cccgtcccgc ctcgaccgtg ctgcgcgtgc cgccggcctt ctggatcacc agcagctcca
      481 ccggccggcc gctcgcctcg acccgcttca tcagcttctg ccgattgaac tgctcgcagc
      541 cgagcgagac cagcagcacc gcccccacgt tggggtgggt ggtcagcgcc tccatcatct
      601 cctcggcata gccgttcggg tagcagccgg cgaaaccgat cagatgcacg ccgtccccct
      661 cgaacggcac cgtcacggcc cgggccacat gatgcgcgca ctcgaccaga taggcgacga
      721 ccacgagatt gcggatgccc ttgccgccat cggcgcgggc gaaagcctgg atgctcgtct
      781 cgctcatgat gcggcctcga agaagtcctc gcgattgacg atgtagtcgc tcttcaggtt
      841 gtgcacgtgc acatgcgccc cggcggcgat cggcgcggtc gtgctgccga tggcgacgcc
      901 gtacttggtg acggtggccc cctgcggcag ctcggcgagg gcgatcttgt gcgcgaacgg
      961 gatcgcatcc cgcaccgtga ccgccaaccc gtcgatctcc accgtctcgc ccggcaccag
     1021 cgcccgcagc gccaccgcca cgttgtcgtc cggcgccaga tgcagcaatg cccgatcgcc
     1081 tgtcatgtca ctcctcccga aaccgcgccc gccactattg gcacggatcg attgccgagg
     1141 cacgccctca ggccggctcc ggcaccagct ccgtccgggc gatgcgccgg gcgatcttga
     1201 agatgccgtc gaggatctgg agcagctcca cctcgcgcac gtagccccgg atcgcgttct
     1261 gcgcgccttt tggaacgtgg ccaaagcgct cgcggctgat ctggctcgcc atgtcggtca
     1321 ccccgtgctt catgttgcgc acggtggtgg cgagctcgtg gtcgtcggtc atcaccgccg
     1381 tgacggcatc gcgcagcgcc ttgaccaccg ccgcgtgata ggcggtgatc cggctcgccg
     1441 cgtcgggggc gatcaccacc cgctcgtcga tccgcttgcg cgccgaggtg gtcatgtcgg
     1501 tcgcgatccg gtccgcgaga tgctcgaggt cgttggtcat ggcgaccaga tgcatcagca
     1561 ccggcgcctg gtccggggtc aagggcgtcg ccgagatgcg gctcagatag gcgatcagcg
     1621 cccggtgcag cccgtcgacg ggcttgtcca tcgcctcgat cgcatagagc gcctggcgcg
     1681 agcccgagag ggcggccggc agggcccgct cgagcatggc gccgacatag tcgcccatcc
     1741 ggccgatctc ccgccgggcg ctgtcgagcg ccatggccgg ggtcgagagc agattgtcgt
     1801 cgaggaacct cggcgccagc ggctcgtcgg cgccgagcgg ccggtcgggc accagccatt
     1861 cgaccagccg ggcgatctgc gtcgtgaagc cgatgaagag gagcgtgttg acgatgttga
     1921 agatggtgtg cgcgttcgcc acctgccgcg gcgcctccgc cgccgccctg gccgagccgg
     1981 tcagattcgt cgccgccggg gagacggcgc gcacgatctc ggcgagctcg ggaatgaagc
     2041 cgatccagat ggcgacgccg gcaacgttga agagcacatg cacgagggcg gcgcggaccg
     2101 cctcgcgcgg cttgccgatg gccgcgagcc cggcggtggc gcacgtgccg acattcgcgc
     2161 cgagggccac cgcgatcgcc gtctcgagcg acatgacacc ctggcccgcc agcacgatca
     2221 cgacaccggt ggtcgccgcc gaggaatgga cgagggcggt gaaggtcatc ccggccagcg
     2281 ccgcgggcag gggatgcgag agcgtcttga tgaagtcgat gaacggcggg taggcgcgca
     2341 acggcgccat cgcctcgctc atcacggcga tgccgtagaa gacgaggccg gcgccgagca
     2401 gcatccggcc gaattcccgc gtgcgttcgc ccttgggcac gaaggcgacg aggaagccga
     2461 gcgtcaggat cggcaaggcg aagcggctga cgttgaaggc gaggatctgg gcggtgacgg
     2521 tgctgccgat attcgcgccc atgatcacgg ccacgctttg cgccatcgac atgagcccgg
     2581 cggagatgaa gccgaccagg atcaccgtgg tcaccgtcga cgagttgacg attccggtga
     2641 ccagggcgcc gaccccggcg ccgagaaagc ggttgcgggt cacccggccg agcaggtcct
     2701 tgagatgctc gcccgcggca cgcttgagcg acacggtcag gacgtccatg ccgtagagaa
     2761 agagcgccat gccgccgaag agcccggtcc agagcgcgaa atggtcgagc tcgacagtca
     2821 cttccggcca ccatcggcct ggtcggcgtc ggctgccaca gtggctgccc gcttgaccac
     2881 gacgaccggc atgggggcga tctgcaccac ccgctcggcc accgagccga gcaggatgtg
     2941 cggcagcccg gtccggccgc ggctgccgac cacgacgagc accgcgccgg cctcgcttgc
     3001 cacctcgacg atgcgaccgg ccggcaggcc cggcacgagc cgggtctcga gcgtcttcaa
     3061 ccgtccctgg tccggatgct cctgacggag ccggtcgagg aactcgccca gcatctgctc
     3121 cgccacgtcg gccagcgggc gcagccagcc atcgtcgggc cgctggtaga agccggggct
     3181 ggcggccggg tcgtggatca cgtgcagcag caggagaggt gcttgggcaa gctcggcctg
     3241 ggcgcagctc cagagcagcg ccgcctcggc gtcgggcgag aagtcgacgg cgcagagaac
     3301 aggtgcggtc acggcagcat cgccccgcgc agcatgaaga agatcatggc accgacgatc
     3361 gccgtcgccg gcacggtgat cagccaggcg gcggcgatcc gcaagagcag cgagcgcttg
     3421 accagctcga tgcggttgat gttgcgcagg cccttgcgct ccttcttgcg cagcgtggcg
     3481 ccccccgact ggcccttcat ctgcctcagc atcagctggc gctcgctcac cttggcggct
     3541 tcgaagcggg cgaggaaggc ctcgaccgcc gccgcgtccg cgtcggcgtg gtgctccctg
     3601 atctcctcga tcatgcgtgc atagctgacc ttgaggtact cgcggaggaa gccgacgccg
     3661 aagacgccac ctacggcgat atgggtggag ctgaccggca ggccgagctg cgaggcgacg
     3721 atgacggtga tcgcggccga gagcgccacg cagaaggcgc gggcccggtc gagctcggtg
     3781 atctccgagc ccaccgtgcg gatcagcttc ggcccgaaga gcgccaggcc gagcgcgatg
     3841 ccgatggcgc cgaccagcat cacccagatc gggatgtgcg ccttgctggc gacgccgccg
     3901 gaggagaccg cgtcgttgat ggcagccagc gggcccacgg cattcgccac gtcgttggcc
     3961 ccgtgcgcga agctcaggag ggccgcggcg aagatcaggg gcacggtgaa gagctcgttg
     4021 accccgccct tggtgttcgg gaccctgggt gccgagctcg cgacatggcg gcgcatgacg
     4081 aaccaggcga cgccggcgag cacgaggccg atgagggcgg cggtgaggaa gctcacgctc
     4141 cagagctgct tcagcccctt gagcaggaga taggtcgtga acgaccaggc catcagcgcg
     4201 atcagcatcg gcacggtccg ctcggccgct gccatcaggt cgctctggta ggtgatgcgg
     4261 cgcttgatga tgtagaggaa gccggcggcg atgaggccgc cgagcacggg cgagatgacc
     4321 cacgacgcga caatccggcc gacctcggcc cagtcggcga tgccgaagcc gccggcggcg
     4381 atgccggcgc ccaggacccc gccgacaatg gagtgagtgg tcgagacggg cgcccccagc
     4441 gccgtcgcca ggttgagcca gagggcggcc gccagcaagg cggcggtcat cagccagatg
     4501 aaggtggtgg catccgggat cagcgccgga tcgatgatgc cgctcttgat cgtgccgacc
     4561 acgtcgccgc cggcgatcag cgccccgccg gcctcgaagg aggcggcgat gacgatggcc
     4621 cccgtcagcg tgatggcatg cgagccgacc gccgggccga cattgttggc gacgtcgttg
     4681 gccccgatat tgagcgccat gtagccgccg atcatcgccg ccgcgaccag cagcagccca
     4741 tgcggccccg aggcgccctg cgccaccgcg aagatcatga tgacgatgac gaacagcacc
     4801 gccgtgccga agcggaagag ctcggcgcgg ttgaccagca gcgcgctctc cagcttgcga
     4861 atgttcggaa cgccagccgc catgatcccg cccccgatag ttgctgcgcg acgtgccacc
     4921 gcggcccctg cgtcacagga ttgtcacgag aatgcaagac gttccgtggc gcgccggctt
     4981 gcgcgagatc aagtgaacgg cgaattggcg ggggcgtggc tggtgggccg ccccttgcgg
     5041 tccgggcgcg aatcggctgg aactcaccga gcagcagagg gcgggcgacg atgggcgacg
     5101 acggcaggac ccgggggctg gcgaacggcc tcaccaatta cggcgacccg gcgttcagcg
     5161 cctatctccg ccgctcgttc gcgagctcca tgggctattc gcgcgacctt ctggccaggc
     5221 cggtggtcgg catcgcccat tcggcgagcg gcttcaacaa ctgccatcgt ctcgtgcccg
     5281 agctgatcga ggcggtgaag cgcggcgtgc tcgccgccgg cgccctgccg ctcgacttcc
     5341 ccaccatctc gctcggcgaa ggcttcctca cgcccaccag cctcaagttc cgcaacctca
     5401 tgtcgatgga cgtcgaggag atgatccgcg cccagccgat ggacgcggtg gtgctgatcg
     5461 gcggctgcga caagaccgtg ccggcccagg tcatgggtgc catctccgcc ggccgcccgg
     5521 ccatccagct cgtcaccggg cccatggcga ccgccatgca ccgcggcgag cgcctcggcg
     5581 cctgcaccga ctgccgccgc ttctgggccc gctttcgggc cggcgagatc gacgaggcgg
     5641 agatcgaccg ggtcgagcgg cggctcgcct ccaccgccgg cacctgtgcc gtgatgggca
     5701 cggccagcac catggcgtcg ctggtcgagg ccatgggcct gatgctgccc atgggcgcct
     5761 cgatcccggc cgtcgatgcc gaccggctgc gcctcgccga ggcgaccggg gccgccgccg
     5821 tcgccctgat cggcagcggc cggcccatgg ccgagctggt gacgcaggaa agcctcgaga
     5881 acgcgctccg cgtgctgctc gccatcggcg ggtcgaccaa tgccgtgctg cacatggcgg
     5941 cgatcgccgg ccgcgccggg ctcgagctcg atctgcgccg cttcaacgag ctgagcgaga
     6001 ccacgcccgt catcgtcgac ctgaagccga cgggtacgca ctacatgcag gacctgcacg
     6061 ccgccggcgg tcttccggcg gtgatgcgcg agctcggcga tctcctgcac ctgggcgtcc
     6121 ccaccgtcac cggcgagacc ctgggcgagc ggctggccgc cgccgatccc tgggtcgatc
     6181 gcaccgtcat ccgcccgatc gacgacccca tccacccgaa aggcgggctc gtcgccctct
     6241 tcggctcgct cgcccccaag ggggcggtgc tcaagcgctc ggcggccgat cccaggctct
     6301 tcgagcacga gggccgggcc gtggtcttcg acggcctcga ggacctggca gcccgcatcg
     6361 acgatccgga cctcgacgtg accgccgacg ctctcctggt cctcaagaac gcagggccca
     6421 ccagcccggc ggcgatgccc gaggccggct acctgccgat cccgaaaaag ctcgcccggg
     6481 ccggcgtgaa ggacatgctg cgcctctcgg acgcgcggat gagcggcacc gcctacggca
     6541 ccatcgtcct ccacatcgcc cccgatgcgg cggccggcgg ccccctggcg ctggtcgaaa
     6601 cgggcgaccg catcagcctt tccgtcgagc ggtgccgtct cgatctcctg gtcgagccgg
     6661 acgagctggc gcgccgccga caggcgctgc cacccccgcc gccgctgccg gcgcgcggcc
     6721 acgaccgcct ctaccaggag cagatcctgc aggccgacga gggctgcgac ttcggctttc
     6781 tcaagggcag ctagactcgg cgccgcgcct gccggccctg catcgcggtg cagggcaaac
     6841 ccctgccgag cagggccatc atgagcaggc ccgggcgcct ctcgggaact ccctgccgca
     6901 ccgccggttc tctcgatgtg gcatgagcgc aagcgggggc gggcgagggt catcggttgg
     6961 caggaactgg gagcaagaga tgggtatcga ggtcgggggc ctgatcggcc tcctcgtcct
     7021 gatcgccgat gtctgggcga tcgtgaacgt gttctcgagc ggcgcctcgg tcgggtcgaa
     7081 ggtggtctgg accgtggtga tcctggtgct gccggtggtc ggccttctgg cctggctgct
     7141 gttcgggccg cgcgcagccg gctgaccctg cccacccgca actccgcccg gcgcgaccga
     7201 ttgccccgag ccgatgcata agcgctcatg tcgggggtgg cggcgttgtc ggcggtcaat
     7261 ggcgacatag ctgtcgatca ggctgatgca aagtacggtc gggccggcca cgcaccggcg
     7321 ccgccggacg aggttcgggc aaggctatgt tggacggctt ctccgcaatc gatctggcgc
     7381 ggttccagtt cgccttcacg atctccgccc atatcgtctt tccggccttc accatcgggc
     7441 tggccagctt tctggcggtg ctgaacgggc tctggctctg gcgccgggac gaggcctatc
     7501 tgcgggtgtt cgactactgg aagacgatct tcgccgtggt cttcggcatg ggcgtcgtct
     7561 ccggcatcgt gatgtcctat cagttcggca ccaactggtc ggtgttcgcc gacaggaccg
     7621 gcccgatcct cggcccgttg atgggctacg aggtgttgtc ggccttcttc ctcgaggccg
     7681 gcttcctcgg catcatgctg ttcggccggg aacgggtggg cgaccggctg cacatggcgg
     7741 ccaccgtcat cgtcgccctc ggcacgctct tctcggcgtt ctggatcctc agcgtgaaca
     7801 gctggatgca gaccccgcag ggctactcga tcaacgaggt gggccagttc gtgcccgagg
     7861 actggtggct gatcgtcttc aacccgtcct tcccctaccg gctggtgcac atggtgctgg
     7921 cggcgtatct gaccaccgcc ttcgtggtcg gcggggtcgg cgcctggcac ctgctccgcg
     7981 atcgtgagaa ccgcgccgcg tcgatcatgt tctcgatggc catgtggatg gccctgatcg
     8041 tcacgccgat ccagattctg gcgggcgatc agcacggcct caacacgctg gcgcaccagc
     8101 ccgccaagat cgcggccatg gaaggccatt tcgagagctt ccccgagggc cgcgccccgc
     8161 tggtcctcct cgggctgccc gacgatgcgg ctggcgagac ccgctacaag ctggagatac
     8221 cgctgctggg gtcgctgatc ctcacgcacg acctcgatac cccgatccag gggctggatg
     8281 cctttccggc ggacgagcgc ccgccagcgg cgctcatctt ctggaccttc cgcatcatgg
     8341 tggccgtcgg cttcgccatg ctggggatcg gcctctggtc ggcatgggca cgctggcagg
     8401 ggcggctcga cgatgcccga tggctgcacc gcgccgccgt gctgatggcg ccatcgggct
     8461 tcctcgccgt gctctgcggc tggatcacca ccgaggtcgg ccgccagccc tacacggtct
     8521 atggcctgct gcgcacagtg gagagcgctg ctcccgtgca ggccgccgcc gtcagcgcct
     8581 cgctgaccgc cttcatcgtg gtctatttcc tcgtcttcgg ctcgggcacc ttctacgtga
     8641 tgcggctgat gggccgggag ccacggagca gcatcggcat cgagcagatc ggcccgacgc
     8701 gcgccgccgg gatcaccccc gtcgccgccc gcccgaccca gtggcatgca agcacagggg
     8761 ggcagccatg agcatcgacc tgcccttcat ctgggtcggg ctgatcgcct tcgcggtgct
     8821 ggcctacgtt ctgctcgacg ggttcgatct gggcatcggc atcctcttcc cgttccttgc
     8881 cgaccgcgag gagcgccatc tggcgatgaa cacgatcgcc cccgtctggg acggcaacga
     8941 gacctggctc gtgctcggtg gcggcggtct cttcgccgtg ttcccgctgg cctacgccat
     9001 cgtgatgccg gcgctctacc cgctgatcat cgccatgctg ctgggcctga tctttcgtgg
     9061 cgtcgcgttc gagttcgtgc atcggaccag gcggatgcag ggcttctggg agctcgggtt
     9121 ctggggcggc tcgctcgtcg cggcgatcgc ccagggcatg gcgctcggcg cgctggtgca
     9181 gggcatcgcg gtcgagggcc gggcctatgc cggcgggagc tgggactggc tgacgccctt
     9241 ctcggcgctg accgccctgg cgctcgtcgt gggctatgcc ttgctcggtg cgacctggct
     9301 gatcctcaag accgaaggcg cgacgctggc ccgcgcccgg cgcttcgcca tgccgctcgg
     9361 cgtcgccctg atcgccctga tcggggccgt cagcctggcc acgcccttcc tgcgacccga
     9421 gtatctcgag cgatggttcg gctggccgtc gggcgcctgg agcgccgtcg tgccgatcct
     9481 gctggcactc gccgtctggc agttgtggac tgggctccgt caccaggagg atcatcggcg
     9541 cccgttcctc gcgaccgtgg cgatcttcat cctgtcctat gccggtctcg gggtcagctt
     9601 ctatcccaac ctcgtgccgc ccgccctgac gattgccgag gccgccgcac ccacgagctc
     9661 gctgctgttc ctcttggtgg gcgccgccct cctgatcccg atgatcctgg cctacacggc
     9721 ctattcgtac tgggtgttcc gcggcaaggt gggtcccgcc gacggctacc attgatgcgc
     9781 cgctggctgt ggttcgcggg gctctacatc ctcggcatcg cggtcatcgg ggcgatcgcc
     9841 gccgcgcttc gagccgcgct gctctaacgg cgcggccggc ggcggaattg ctcgtcgatc
     9901 accccgagca cgaaggccac catcagcgct tccattggcc gcggcggggt cggggtcgtc
     9961 aacggcggga cgggtgagcc agacgggtag tgccgccgca ccgaccgtcg cctgccgacc
    10021 aggaagagga tgatggcgcc gagcgccagg acggtgccga gcacccccgc cgccagcggc
    10081 tcgcccagtt gcctcgccag gctcatgtag aaggccatgg cgaagaagat gatcgccagc
    10141 ccgccgagca aggccgcagc gccccagaaa acgagctggc ggcgcatctg ccgcagatgc
    10201 gccgctatcg gctggagtgc tgccagcatg cgttccctca gcgtcgatcc aggaacttgc
    10261 tcaggatcag cccgacgccg aaggcggcga gcaggctggt cagcggccgg tcatggaccg
    10321 tgcgctcgac cccggcgagc gcgtcgtggc cccgcgcata catgttgtcg cccagttgct
    10381 ccagctcgtc gcgcagctcg ccgagcttgg cgctgccggc ctccttcgcc tcctgggcgc
    10441 tccgcttgcc gatgccggcg atcgtgctct tgatgctggc gatgtcctcg cgcaggcggg
    10501 ccagatcttc cctgagtgcc gccagatcct tgtcgccgtc ggtgtcacgt gcggccatgg
    10561 ccgaaatccc tttcaaaagt catttcacgg acaagaactg ccaattgcgg ccgaggttcc
    10621 gggggcctcg ccgaacacca ctagcggcac tgcgggcagg acgccagcga tcccccgcac
    10681 cgcagcccgc tctcccccgt gcttcggcga agcgtcgccc gccgatggaa ccgggtgccg
    10741 cgaccggcgt tgatgcccca gaccgccgtc gtgccggttg ttgatgccga gccggcgacg
    10801 acggcggaac ccaccggaag gagactggca tgctgtactg ggctttggtg ttctttgtcg
    10861 ttgccatcat cgccgcggtg ttcggcttcg gtggcatcgc ctccgcttcg gctggcatcg
    10921 cgcagctgct gttcttcatc tttctggcgc tgctggtcat cagcctcgtc gtctacgtga
    10981 ttcgtgggcg gtcgccgccg gtgacctgag cgtctttccg gtgttaaagg gcagattgag
    11041 gccggctttg ccggcctctt tttttgccct ctgcttggcc aggtacgcaa ggtcaactat
    11101 ccccgccacg ttccggccat gactattgat gcttcggctt cgtatccggc ggaacccgag
    11161 gggcgctgct cgattattct atccagttgg gggcacagcg ccgcaggggc ggggtgatgt
    11221 cccggaccga gaaggagacc gagtgatgag aaagatcctg atgagctctg tggcctccat
    11281 ggcgctcttg ggcgttgcgg ccggcttcgg cggaccgctg ctggcccaga ccgacgcgcc
    11341 ggcggcgacc gctcaggacc agaacgccca gaatggcgat acgcaggagc agggcaacgt
    11401 gccggccagc tcgcagggcg acgccacgca gtcggccgag acccagggcg gcgacgccgt
    11461 cggggctgct ggcgcgaccg gcgcggatca gccgctcgcc agcgcaccgc cagaggagat
    11521 gcgttaccgc tccatccagg atgccgaggc gggcagcatc accttgccca acgagatgac
    11581 caccgaggag atcgtcggcg ccaatgtcta cgacgccaac gacgagtcga tcggcgaggt
    11641 ggtggatctt ctggtcaccg aggatggcca ggtgagccag gtgctggtgg atgtcggcgg
    11701 cttcctcggc atgggcgagc atcgcgtggc gctcgacctg gacgagatcg agttcgtcga
    11761 cgccgacggc gtgctgcgtg ccagcgtcga tgtgacggag gaagagctcg aggcgatgcc
    11821 ggcgttcgag cgggccgagg acaactattg ggtcggtagc gccggcccgg tgggctcgcc
    11881 ggtgatgccc gacacgaccg ccgagcagac ccggaccgcg gccaccgagg cgccggccgt
    11941 ggacgacacc gaataccgct cgtgggatca ggccggcgaa gccgacatga tgatgggcga
    12001 catgtcggcc aaggatgtcc ttggcgcgac ggtctatggt ctcgatggcg acggtatcgg
    12061 cgaggtcgcc gatctgctga tcggccccga caacaacatc gaccggttga tcgtcgatgt
    12121 cggcgggttc ctcggcatgg gcgtccatac ggtggcgctg ccggccgacg agatgcagat
    12181 cgctgccgtg gacggcgagc agcgcatcac cgtctcggca accaaggagg agctcgaggc
    12241 catgccgtcc tacgagcggg atgccgacgg caagtggctc gtgaacgagt gacctcgcga
    12301 ccggggcgcg gcattcgtcg cgccccgcca tctctcccta cctcagaaag gaacgaccat
    12361 gaactgggat atcatcaagg gcaattggaa gatctttcgc ggcaaggccc aggagcagtg
    12421 gggcaagctc acggacgacg acctcgatgt cgcggaaggc aactacgatc agttcgtcgg
    12481 ccgcatccag aaaacctacg ggatcagccg ggacgaagcc gagaagcagg tgaaggactg
    12541 gcagagccgc cagaagccgc tctgacctga gccatcgcgg gcgcccccgg cgcccgcgat
    12601 gcagccaaag gaaaaccccc ggaccgcgag gtccgggggt tttgccgttt caggatgcag
    12661 tgccccggtc aggcacggcc catcgggcgc ctcgcccagc tcgcatccac ggcgccatcg
    12721 tccctgtgca gatgagcgcc cggctcatcg gcgtcgggca cgaggcgctc gagcatgcgg
    12781 tccggcaggt tgcgcgccag cctcgcctcg tccaccggca gctcgtcctc gagcagcatc
    12841 ttcttgagga tcgccctggc ccggttgacc cggctcttga ccgtgcccac ggcacaaccg
    12901 cagatctcgg caacctcctc gtaaggcagc tcctcgacca cggcgtagat caggacctcg
    12961 cgatgggtcg ccgaaagcgc gccgaaagcg cgcttgagcg cgtcgatctc gatcatcgac
    13021 tcctgatagg ccggcaccga ccagcgcgtc tcgagatgct cgtcgtagac ctcgctcgtc
    13081 tggtacttgc gcgccttgat gctcaagaaa cggttgcgca ggatacgaaa aagccaggcc
    13141 ctcaggttgg tgcccggctg aaactgatgc tgcgcctgga gcgcattgac catcgtgtcc
    13201 tgcacgaggt cgtcggccaa tgccgcgtcg ccagccgcga ggctgcgggc gaaggcccgc
    13261 agatgcggca ccagggccac gacgtcgtcg cgaaaaacag tcatgtcgtt gctccctaca
    13321 cgttcttcga caagcttcaa ccgtcggcgg gctcgaaggt tccctcgctc gacgattcga
    13381 atacgtcgaa atcgtgcaag ggaaaggtca gtcgcaccgt cgtccccgcc tggtgctcga
    13441 tcacgcactc gccgccgagc tgacccgcca gcatccgcat cagccgcagg cccatgccct
    13501 cgagagcctg cgcgcgatcc tcccggccct gcccggcgcc gacgccattg tccgcgacga
    13561 cgagggtagc gcagtcgccc tcgacggtaa aatcgatccg cacctcgggt gcgtgatggg
    13621 cgccggggaa gccgtgcagc tgagcgttgc cgacggcctc ggtgacgaac agggcgagcg
    13681 cgatcgcctg gtcggaggcg atctcgaccg gcgccaggtt ggtgcgaaac gcgatgccgc
    13741 gatcgtcgcc ggtgccgagc gcggcgagga agtcgagcag gtcctcgacg aaggtgtcga
    13801 ccctgaggct ttcggcggca ggctgctcgt agagatggcg atgcaccagc gccaatgcac
    13861 gaatgcgcac ctgcgcctcg tccatgaccc ggcgcgcctc gaccgaggcg gcgctgcggg
    13921 cgcgcagccg caggaggctg gtgacgatct gcaggttgtt cttgacccgg tgatggatct
    13981 cccgaagcat catgtccttc tgctcgagcg agctgcgcag ctcctgctcg cgatgctcga
    14041 ttcgggccgc catctcggcg aacgagccgg ccagttcctt cagctcctcg ggcgcccctt
    14101 tggtgtccgg ccggatcgcc ccgcggccgc ggccataggc tcgcgtggcg gcgacgagcg
    14161 tgcggatgtg gcggttcacg aggtagtcgc tggcgatcca gatcgcgacc acggccaggg
    14221 ccagcatcac cgtcggcgag aggatgccga tcagcaactt gctgtcgagc cagtcccagc
    14281 gcggtggcgc cgcgaagccg acgaccgcga agagggcgcc ctcggcgacc ggcgccgaga
    14341 cgaagagccg ctgcacgccg tcccgggagg tgagctccac gacctcgcgc gggctcgtcg
    14401 ccagcgcgcg cagatcggcc atcgccggcg ccagatcagg cttttctccg gcgtccgcga
    14461 gctcgccgcc gacgatcatt ccctcggcat cgaagatgtg aaccgccgtc ccgtcgggca
    14521 gatcgatgcc acgcatcgcc ctggtgaaga ctgcgagatc gaggcccacg gcgacgacgg
    14581 attcgagcgc accgccgacc ccgttgcgaa ccgcctcggc caccacgatg gtttcgcgct
    14641 gattgaaacg agagatgagc cgctcgctga cgacgaagtc ccggcccccc gccagctccc
    14701 ggaagtaggc gcgatcggcg aggctgacac cgacgctctc gggccggctg ctgcagcgga
    14761 tttcgcccgt ggcgtcgaga atggccagac tgaggtagat cggattctgc ttggccacgg
    14821 cggccagggc cgcgttgcag gatgcttcgt cgttttgcag gctggaatcg cggacgagcg
    14881 tgtccagcag gttgcgcgtg ccgtccagca cctcgcgctg ataggccacg acgatgtcga
    14941 gcagactcgc cgccttcctc tcctgctcgt gcgcctcggc cagataggcg ctcccggtct
    15001 ggtagaccga gtagagcagc ggcggaatga gcacgagccc gagcagcagg gccatgcgcc
    15061 ccttcaggct ggcgggctgg cgccagcgtc ctcttccggt cggtgcgtcg ccgtcgaggt
    15121 cgccggcaat caagccggct gctcccgtcg cctccgccgg gcgctggcca gggcctggga
    15181 gatcgtgatg tcgagcgtgt cggcatcgaa gggcttggtc acgagaaagg tcggctcggg
    15241 ccgcgttccg gtgagcaggc gctcgggata ggcggtgacg aagacgaccg gcagctccgc
    15301 ctcgcgcagg atctcgtgca ccgcctccag acccgagctc tcgtcggcga gccggatgtc
    15361 ggcgaggatc aggtccggcc ggtgctggcg cgacagcgcc accgcctggc gatgggtcgt
    15421 ggcgatgccg acgacggtat gcccgttgcc ctcgagcgag gtggcgatat cgagggcgat
    15481 caccggctcg tcctcgacca cgagcacgcg gctcggcggc tgccggcgca gctcggcctt
    15541 ggcctcgtcg agcagcgcat cgacctccgc ctcgcccacg gccatgatct gcgccacctc
    15601 tgaccgatcg aactcctcga gggtggcgag cagcagggcc tggcgaaagg gcggcgcgag
    15661 ctcgtgcagc ctcgtctcga gggccagggt cccctcgagc aggtcggaat cgctcggctc
    15721 ttccgcgacg gcggccggca gccgactgcc gttccaggta tcgtgcagcg cccgatagag
    15781 cgccagccgg gggtcatgaa ggcgctcctc ggcgagcctg tctgcgagcc ccgtcaatgc
    15841 cgccgcaacg tcccggtcgc cacgcgcctg gtcaccagtc accgcccgcg cgtaacggcg
    15901 caggaacggc aggacgcgcg caatctcctg gcccggctgc ccccccgtca tctcaggccc
    15961 ctcccaccgg gccgccacgc ttctgcgggc ggttgccgtt catcttttcc tcgtcttcgc
    16021 gatcctcggc cgcgctggcc gactcgtcgc gcggctcgaa ctgctcgatc agcaagcgaa
    16081 gccgctccgg cagctcctcg tcgagtaccg ggtcgtagat cgcatgcagc tgcttgccga
    16141 gccagcgatc gaagccggcg tcctgggccg agcggcgctt gcgcgcgacc cctcgggcct
    16201 ggtctccggc cgcaccgtcc ggctcttttg cccccttgcc catcacgtct cgcccgccgc
    16261 tccgtcactg gtccagtagg ttgaaaccgc tggcgcggcc aaaggttcca gctggcacgc
    16321 cttacttata gcagcagccg cccgcacggg cgagtggccc gctcgtttca gatcagcggc
    16381 cggcgcagcg gcttctccgg gtcgacagcg gcgacctcgg ccagagccgg aagatcgacc
    16441 accgtgaggc ggcgaccgtc gaggcgcacc aacccggaat cgaccagccg ccggagcacg
    16501 cgactgacgt gaacggtcga gaggcccagc agatcggcga catgctgctg ggtcagcggc
    16561 cagaggatgg tgccgtcctt gaccagacgc agcccgtcga gctggcgata gagatgcacc
    16621 accagggcgg ccaatcgttc gaaggagggg cgctgcccga cattgctcag cgccacctcg
    16681 acggtccgct cgccgcaggc cgcggcccag gtgaggtcga aggcgagctc gggaaagctc
    16741 tcgtagatcg tccagaagcg ctcgcgcgag aagacgcaga gacggacatc ggtcagggac
    16801 tgcaccgaat gctgcatggt gcccagcatc tcggtctgca ggccgatgaa atcgccgggc
    16861 agcagcaggc tgtggatctg ccggcgacca tcctccatca gatcgtagcg aaagcaccag
    16921 ccgccgaaga tggtgaagag atgggcgctg tgcgtcccgg cgagcaggat atcgctgccg
    16981 gcctgcacct ccaactcgcc gatcttgaac ttctcgacga aggcgagctc cgcgccgctc
    17041 aggcagcgga agatcggcag ggcacgcaag gcgcaattct ggcatggggt ggtggtgctc
    17101 aacggctcga gtcgcgccaa cctcgtctcc catgggcgat ccgctgcaga agagacgagc
    17161 gaccgaccaa catatgttaa cgaccagggg ttccacctgt gtcacccaat cttgcgtaca
    17221 cgccgtccgc cgtccgctgc aagccgcagc gtcggtccga cctggaggaa gcatgcgcaa
    17281 gccgcccagg gcgcgacacg ctcgcgtcgc cacccgtcac cgcaccgccg acgacaggag
    17341 cccggcatcg ctcgagctga gcgagctgcg gcaccgcacg cagaatctgc tgcagaccat
    17401 ccagggcctg ctcggcctcg aggcgcggcg cgccgagcac gcggagacgc aggagcggct
    17461 tcatgatcta gcgctgcgcg tcgacctgct cgccggtctc caccagcgcc tgaacgctct
    17521 cggtcccggc ggcgacgttg atgccggggc gctgatccgc gagtcggccg aggcgctcat
    17581 cctgccgaat gagagggtca tcgccctgga atgcgaggtc gagaagctgc cgctcgacgg
    17641 ccagcgggcg cagtccctgg ccctcctcgt ccacgaggcg ttgaccaatg tgacccgcca
    17701 cgcctttcag gaggcgcaga cgggcagggt ggtggtccgc gcccgccaga caagggacgg
    17761 cggctttctc gtcgaggtcg cggacaacgg ccgcggcatg cgcccgccac cgggctccgg
    17821 cgggctcggt ctgcggctga tgcgaacgct ggcgctccgc ctcggcggcg agctgcggct
    17881 cgaggacggc gccccgggtc tgaagctcac cgtgctcgtg ccgccaccgg agccggtcgc
    17941 cgccgacacc gccggcccgt ctgccgcccg cgtcgccgca ccgccccgaa agtgcgcggc
    18001 cgccgtctag gggcggcgcc gcccatttga gaagatacga ggacgaattt cacgcccgtg
    18061 gccgcggatc ccgcttcagc cgtccatccg cacgccgccg ttcacgtcga gcacggtgcc
    18121 gcagacatag ctcgccgccg gcgaggagag aaaggcgatc ggccaggcga cctccagggg
    18181 ctcgccgagc cgcccgaccg ggatggcgct cgtgctcacc ggccggccgt ccatgagagg
    18241 tgttctgatc gaggcggggg cgacgccgtt gacgatcacg ccccgcggcg ccgcccgccg
    18301 tgccagccag cgcacgaagg catgcagccc gcccttggcc gccacgtagt gcggcgaggc
    18361 gatgagcccg ccggtcttgc cggcgagcga gccgacgaag accatccgcc cgccgccgcg
    18421 cgccgccatg ccgggcaaga gcgcccgggc gaggcggatc ggggccagca ggtcgacccg
    18481 gatcacctcg tcgaagaccg cgtcccagcc cgcctcctgc cagtcgtcga aggggcagat
    18541 cgcggcattg gcgaccagcg cgtcgaccgg gccggcagcg gcgacgagct cgtccagcgc
    18601 ctcggccgag cccagatcga cacggaaggc gctgcctcgg agctcgttcg cgatcgccga
    18661 ggtgtcgtcg cggtcggcca gcagcaccgt cgctcccagg gacgccagca ggcgggccgt
    18721 cgcctcgccg atgccggcgg cggccccggt gaccagcacc cgttgcccgt ccagccgcag
    18781 cccgtcccgg accggctcag gcacgcccgg cctcgcccgc ttcgggcggc gggccggtcg
    18841 cccggcgccg ctccgcctcg gcggtggcga gacgctgctc gcgccgccgc gtcatccagg
    18901 cggcgagccc gaccgccagg atcagcatcg caccattgaa gatcggctcg acatagaagg
    18961 gtgccccgag ctggctcagg ccggcgacgg cgacggcgag gaccgcgacg gcgaggatgg
    19021 tgccccaggg gttgggccgg ccggggcgga tcgcggtggc gccgaggagg gctccggtga
    19081 aggccggcag cagcagctcc tggcccaccg tgctctggcc gaccctgagc tgcgcctgca
    19141 gcacgatgcc ggcgatggcg gcgatgacac ccgaggcgac gaaggcgagc acgacatagc
    19201 ggtcggccgg gatgcccgag agcacggcgg cgcgcgggtt gtcgccgatc acgtagagga
    19261 agcggccgag cgggcgatgg tcgaagacga tccagagcaa aaacgcgatc gccagcgcat
    19321 agatgagcgg caggggcaca gccatgccgg gcacgaccgc ggcgaggccc gtgaacccat
    19381 cgggcaaagg ccccaccacc tgccggccgc ccgtgtacca ctggttcacg cccaaaagca
    19441 gcgtgccggt gccgagcgtg gcgatgaagg agttgatcct ggcccgggtg acgagcaggc
    19501 cgttgacgag cccgaccagg gcgccgatgg ccagcacgat ggcgcaggcc gcgggccagg
    19561 agaggccctg ctgggtctgc agaccgttgg ccagcacctg gccgatgccg acgatcgagg
    19621 cggccgagag gtcgaactgg ttgcaggcga gcgggatcat gacggccaag gccatcatgg
    19681 cgttgatcga ccggctgctg gccatcgact tgaaggtgaa ggcggtcggg aaggtgtcgg
    19741 gcttgatcac cgtgaagagc acgacgaggg ccaccagcag caccagcagg tgccagaccg
    19801 agagaaagcg caggagtccg gactgctgct tcatgcggcg gccccggtga gcgcctcgcc
    19861 gccggcgagg cgggtgagca gtggcacgga aagccgttcc cgcggcacct ccgcggtgat
    19921 ccggccgcgg tcgaggacga gggcgcgatg cgcgatgccg gccacctcct cgaagtccga
    19981 ggatatcagc agcacggccc ggccctcgcc gaccagccgg tcgaggatgc ggtagatctc
    20041 cgccttggcc cccacgtcga cgccgaaggt cggctcctcg aggacgagca ggcgcatccc
    20101 ggtctcgacc cagcgggcga tgaccacctt ctgctggttg ccgcccgaga ggttgagcac
    20161 cggctgctcg ggctcggccg gccgcaccgc gaggcgctgc atggccgatg ccgcccgcga
    20221 ccgctcgctc cggtcgttga tgcgtgcccg gccggcggtc aagggcgggt tcatgaagag
    20281 gttctcgcgc acggcgaggc cgggggcgag gctctcctcg acccgccggc tcgagacgaa
    20341 gccgacgccg cccgccgtcg ccgcggcggg cgaggcgggc cgccagggcc ggccggcgag
    20401 acgggccacg ccgccgcggc cccgaacggc gccgaagacg aagcggccga gctcgtggtg
    20461 gcccgcaccg cgcaggccga cgagggccag gatctcgccc tcgtgcaggg tcatggagac
    20521 cgggccgacc ccgtccagct cgagccggtc gagctcgagc agcggcgccg cgtcgccaga
    20581 gccgggcggc ggctggaaga gctcgtccag gctgcggccg acgatcagct cggccagcat
    20641 cgccgaggtc gtgccggcga ccggccgcgt cgccacatgg cggccgtcgc gcagcacggt
    20701 gacgctgtcg gcgagggccg ccacctcgtc gaggcgatgg gtgacataga gaatgccgag
    20761 gccctcccgc ttgagccggc gcagggccgc gtgcaggcgc tcgacgtcgc tcgccggcag
    20821 ggccgcggtc ggctcgtcga gcaccaggag gcgggccttg ctggcgagcc cgcgggcgat
    20881 gccgatgagc gcccgctcgg ccgccggcag ctcgccgacc ttcatgtcgg gatcgaggtc
    20941 ggccgccatc agctcgagca cctcgcgtgc ctcgcgctgc acgcgcggcc agtcgatcag
    21001 tccgcggcgc aaggggaagc cgcgatagag cgcgatgttc tcggcgacgc tcaggccgtc
    21061 gaccagcccc agctcctggt gcacgaaggc gatcggcagc ttcccggcca tgggctcgac
    21121 ggcgacacca tccatcgtga tccggcccgc gtccctggca tggaccccgg ccaggatctt
    21181 gatcagcgtc gacttgccgg caccgttctc gccgagcagc gcccggatct cgccggcctc
    21241 gagggcgagg tcgacggcgt cgagggccag cgtgccgccg aagcgcctgg tcagcgcctc
    21301 ggcggcgagc agcggcggct gcaaggtgcg gctcgtccgg gccgaacggc tactcgatgc
    21361 cccagatctt cttgtaggcg cccttgtagt ccatgctcgg cctgaagccg ttctggtcgc
    21421 cgccctcggc gtcgatattg tccgccgtca cgagataggg cttctgcacc cagcccgacg
    21481 gcggctcgcc gtggaaggcg cggttgaact cgtcgatcgc ctggaagccc tgcagctcgt
    21541 agggctcgct ggcggtgacc cgctggtact cgccgccggc acggatgcgc tcataggccg
    21601 agcggttgcc gtcggccgag accaggatca cgtcggccgg gtcgacgccg ccggcgcgga
    21661 gcgccggcac ctggaagtcg gcggtgtagt ccgccaccga ggtgatgtag agcggcgtgc
    21721 cgtattgctg aacccaggcc gcgacgagct gcggctggcg ctgcgccgcc tcggcgatcg
    21781 gcgtgttgga gaactccagc atctcgcact cctcgcattc gaggatgcgg tcgcgcgtcg
    21841 cctcggcctt ggcgcaggcg atggcgtact cgcagtgcgt ggtgacgacc acccggccct
    21901 tgccgtcgga atcggcgatg atccagtcgg cctgggcgcg gccgatgtcc ctggggtcct
    21961 gctggatgtt gtagaagacg cccatcgcct cgtccggccc cggaaaggcc aaaccgtgaa
    22021 tgccgaggac gacgatgccc tgctccttgg ccgcctgcac cggctcctgc aggctggcga
    22081 tgtcggccgt ggtgacgacg ccgtcgacct gcagggctat cgcctggttg aagccctcga
    22141 tccaggtctt cgggctgccg cggccgtcga tgatcgtgac ctcccagccg accagctcgc
    22201 ccgcctcctt gaccgcctgg ccccactcgc gcgacgcgtc gttctgctcg tcggtggaga
    22261 gataggcgat cttcttgccg ggctccggct tcggcccctc ggtcgggccg tcccagaccg
    22321 tctgcggccc gctccgctcg gcgaccgtct tcttcgcctc gtcgagcacc gattgcccga
    22381 gggcgaacga ggatgtgccc agcaccagca cggcacccaa ggcagcgatt ggcagcttca
    22441 tgagggctcc tccgcgagcg atctttgatc ctagcctaca ggggacctgt ctgataggtc
    22501 aatagctcga cagatgagga agcctgcagg tcgaggagcg gtggagacga agcggacggc
    22561 agccggggac gggcgccccg aaaggacggt gcaatcgttg ccgcaagcac cgatcaggcg
    22621 ccgcaaggtg cacgaggagg tggccgagcg gatcgagcac atgatccgcg aaggcgacct
    22681 ccagcccggc gatttcctgc catcggagcg cgagttcatg gccgccttcg gcgtcggccg
    22741 gaacgccgtg cgcgaggcga tgctcgcgct gcagcgcatg gggctggtcg ccgtcagcag
    22801 cggcgagcgc tcgcgcgtgg tgcgcccgac cgccgaggcg atggtcgccg agctcgccgg
    22861 cgccgcccgg ctgctgctcg ccgacgatga cggcatgcgc cagttccagg acgcccggtc
    22921 gctcttcgaa ggtgcgatcg cccgccgggc ggcaaggctc gccacaccgg cggacctcgc
    22981 ccggctggag gagatcctcg accgcaaccg gaaggcgatc ggcgaccatc gggccttcat
    23041 ggcgatcgac gtcgagttcc acatggcgct ggccgacatc tgccggaacc cgcttttcac
    23101 cgccgtccac ggcgccgtcg tcgaatggct gatcgaccag cgtgccatct ccggcaaggc
    23161 ggcgaccgcc ggcgaggcgg cctacgccgc ccatgtgcgg atcctggagg ccatagccgc
    23221 gcacgacggc gccgcggcac agcgcgccat ggaggatcat ctggccgccg tggccgagct
    23281 ctactggcaa atccgcgcca ccaacgacac gaggggagcc tgaagcatgg ccgagtatcc
    23341 gggcagcgag cacgagcggc gggtgccggc ggcggacctc gccgatggcg tcgcccgcat
    23401 cttcgaggct gccgggatgg gtccggcgga tgccgcgctc ctcgccgaca gcctcgtcca
    23461 tgccgacctt cggggcatcc acagccacgg cacgctccgg gtgcccgact acgtgaagaa
    23521 gctcaccacc gacggcgtcg atccaagggc ggtgccggtg gtcgagcggc gggcgggtgc
    23581 cgccatcgtc atcgacgggg ccaacgccat gggccagatc gccatggacc tcgccatgcg
    23641 cacggtgatc gaggcggccg gcgagcacgg catcgccatg gccgcggtcg gccgcagcaa
    23701 ccattgcggg gccatggact actgggcgat gcgcgcgctg ccgcacgaca tgatcggcat
    23761 cgccggcacc aacgccctgc cgaccatggc gccctggggc ggggccgaga agctcgtcgg
    23821 catgaacccg ctcgccatcg ccgtcccggc cgcggaggag cccgccatcg tgctcgacgc
    23881 cgccttcggc atgacc
//
LOCUS       DAOTFI010000037        23671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2513_length_23671_cov_6.364033, whole genome shotgun sequence.
ACCESSION   DAOTFI010000037 DAOTFI010000000
VERSION     DAOTFI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 23671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23671
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2513_length_23671_cov_6.364033"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5558_05480"
     CDS             <1..478
                     /locus_tag="P5558_05480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRY23792.1"
                     /translation="GASFLYLGRNYRLRFVSDTSRPLVLRNGWFELDERVTAEGGAPA
                     ARKVFRDFYTTRAASLLEERIALFARRVDVRHGPVAVRELGYHWASCGPSGSLNFHWK
                     IMMAPSTVIDYVVAHELCHLKHRDHSVAFWNEVDKVLPSWRDKKDWLRRCGAALDL"
     gene            complement(831..920)
                     /locus_tag="P5558_05485"
     tRNA            complement(831..920)
                     /locus_tag="P5558_05485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(884..886),aa:Ser,seq:cga)
     gene            1050..1667
                     /locus_tag="P5558_05490"
     CDS             1050..1667
                     /locus_tag="P5558_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23793.1"
                     /translation="MRPCTPALPFALLSLLVATAPLAAADEIREQLDLAIELYEEGDL
                     AGSITELQYAIGEIQGRLGSAYAETFPPAPAGWTAEAPSQEASPAFLGGGTMVSRAYR
                     EDGGSGTMNAQLMIDNPMIQGMAALMSNPAIISANPNMKRIRLGRENAVLDFDQAAGR
                     GEITFLMGGGRAMLKVEGSGLASGDLLVETLEAWDRQGLEDVAGL"
     gene            1677..2423
                     /locus_tag="P5558_05495"
     CDS             1677..2423
                     /locus_tag="P5558_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY23794.1"
                     /translation="MIDDPAFYLAAIPAVLIFGISKGGFGGGLGIAAVPLMAIVVSPA
                     RAAGILLPLLVLMDLIGLFAYRRHWDRRVVKVMLPGALLGILIGGLAFGYLDDDVVRV
                     VLGLIAVGFTADYFLRDKSRIAARPQRRVAGAFWGCVAGFTSTIAHAGGPPANIYLLP
                     LKLDKSIFVGTMVVLFSAVNAAKIVPYASLGLFDAANLTTSLVLAPVAAIGMLAGIWL
                     HGKVDQLLFYRLCYVFVLLTGLKLVYDGLT"
     gene            2926..4308
                     /locus_tag="P5558_05500"
     CDS             2926..4308
                     /locus_tag="P5558_05500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY23795.1"
                     /translation="METIVGEDFSLVQSLFLSAQARPPVVVWTPRPVVLRSPLLERLL
                     DRWSVLAVGSHPPSPDKAAAFIGCELADWTMQLDLEQRADDDPGPGAFVYRHYGAGIA
                     RQYGRDMTGLRPDAFAGHIGRFFSAVYDAVMRRPEPVFTEHEPPAAVLVRSWRRLVMP
                     FCDPRGQVVRFVVGNIPESPVGTLLDVMHDAALVTDGQGRVRLANIAASTLLGTPSGR
                     IVGCSIDELVPELRPLIAGASGEIEGVSSFGRREIGFVRDGRPLVLDVSIGGTRVAGE
                     PLFVLVLRDQTERVARERSLALEVTRDPLTGILNRRGFHERLAPLLLPRRRRSDGFAL
                     LVIDIDGFKLLNDRHGHAAGDKVLQATAERISQALRGDDLFARWGGDEFVLALNGVTE
                     PAALMAAADKIRRALDPELTAERPVGLSMGGAFHPLHGRDFESVFAAADKALYAAKAR
                     GGKCLEIAVG"
     gene            complement(4327..4506)
                     /locus_tag="P5558_05505"
     CDS             complement(4327..4506)
                     /locus_tag="P5558_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23796.1"
                     /translation="MSQENHRHEQADHVREAVAEHLEDLEDRFTAEAALLAHRQSNEP
                     ALTLDDLDAERDLLR"
     gene            complement(4532..4894)
                     /locus_tag="P5558_05510"
     CDS             complement(4532..4894)
                     /locus_tag="P5558_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY23797.1"
                     /translation="MTHWAIHICGSGGGGTCRRAESGMEKEKMTTFGEDLIEAMGEAL
                     AHAKGTGPAITHEPVDPREIRTRAKLTQAQMAPLMGMSLSGYRKWEQGQRRVSGPAAT
                     LLRVIEKEPAAVQRALSA"
     gene            complement(4909..5757)
                     /locus_tag="P5558_05515"
     CDS             complement(4909..5757)
                     /locus_tag="P5558_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY23798.1"
                     /translation="MPDPASDCLLELTDVRRLYRLPRQRLLGPAPRRVALDGVTLRLW
                     AGETLGIVGESGSGKSTLGRLAVGLERPDSGTVRFQDADLWATGRPGKRRLRRDAQMV
                     FQDPYGSLDPRQKIAQIVAEPLGLLHLGRAAAAERCREALAEVGLGAEALDRYPHEFS
                     GGQRQRIAIARALITRPALLVADEPVSALDVSVQAQVLNLLAELQRRHGLALLFISHD
                     LAVVAHLADRIAVMQAGKVVEEGPRDQVLDAPSHPYTRNLLAAVLPPDPAKARARLGL
                     VRKRRA"
     gene            complement(5750..6736)
                     /locus_tag="P5558_05520"
     CDS             complement(5750..6736)
                     /locus_tag="P5558_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23799.1"
                     /translation="MTLLEIEGLTLDLPTPAGTLRLLDGIDLALNAGDWHGIVGESGC
                     GKSLTALSIMRLQPDRTRLGGRLVFDGRDLLGLDEPALGRLRGAEIGMIFQEPMTALN
                     PVRTIGWQIAEGLRLDGRLGRADIAARTRDAMRRVGLDPEQFSPKLYPHQLSGGQRQR
                     AMIAMVLARRPKLLIADEPTTALDVTVQAEILKLIAEIADETGMALLFITHDLGVVAA
                     IAEAVHVMYAGQIVETGPTDLVFDELAHPYLRGLFDALPTALGEAGRGRLAAIPGQVP
                     EPGGAAQACSFAPRCHRADALCHSVRPVLAPLEAPAHRAACFHPLARGRDRA"
     gene            complement(6733..7551)
                     /locus_tag="P5558_05525"
     CDS             complement(6733..7551)
                     /locus_tag="P5558_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23800.1"
                     /translation="MTRHHHLWIGGALSLLLLAAALLSLVWTPYPVDVIDVANRLAGA
                     SPDHWLGTDPYGRDILSLLLAGARNSILVAVVAVTIGVGLGVPLGALASAAGGWLDEL
                     VMRLSDFAFAFPALLTAVMITAIWGPGAVNAILAIGIFNVPVFARLTRGAALGLWRRD
                     FVAAARLAGRGELAIATSHIIPNLSSLVIVQVTVQFALAILAEAGLSYLGFGAQPPDP
                     SWGRMLNEAQTFLFIAPQLAILPGLCIALSVLGLNLLGDGLRDLLDPRLRRLRQ"
     gene            complement(7544..8488)
                     /locus_tag="P5558_05530"
     CDS             complement(7544..8488)
                     /locus_tag="P5558_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23801.1"
                     /translation="MAPWILRRLLTLLLTLLAATVVVFVVLELLPGDPAAVILGTGAR
                     EDTLAALRAQLGLDRPPLERYLAWLGGLVQGRLGQSYTYGVPVAELIAERATVSVPLA
                     LASILLSTAIAIPVGVHAASRRGRLADTLLMSVTQLGVAIPNFWFAILLILLFAVGLG
                     WLPAGGFPGWEAGFWQAMPSLLMPALALALPQAAILARLTRSAVLDALAEDYVRTARA
                     KGLSRGRAIWQHAVPNALIPVVTIMGLQFSFLLAGTIIIENVFYLPGIGRLTFQAIAQ
                     RDLTTVRDLVVVLAAVVILVNFVVDVLYVLIDPRLRHD"
     gene            complement(8511..10499)
                     /locus_tag="P5558_05535"
     CDS             complement(8511..10499)
                     /locus_tag="P5558_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY23802.1"
                     /translation="MTTVTPVGADPATIEALGKAFGKLGKVRPVGELASMRQKLTGAD
                     RVLIVDQALIIIEQLYTHLHMKRVRHAIDPAQALRVLRDQAAGLGDLRFHSEMLRIFK
                     SLRDIHTAYTAPPPFAEQTAFLPFLLEPCTDAHGTTRYLVTRVLAHFDHPTFRPGVEI
                     THWHGAPIARAIAINAAREEGSNPESQAALGCLFMTVRWLGACLVPDEDWVILGYRHP
                     SGEAEIRLPWSMLELNDPGLRFAIQTVWLFEKDTSDGEPQPPHSLNHATNIRTQVEQV
                     TRRHLFAGEQKKAPARRRAPDDLASHLPDLMTAERLADPKGGEPYGYLRLRAFEHDDV
                     AEFKDEFRRLLGLLPRRGLVIDIRGNPGGIVDCAEGILQFLTHRRITPLPFQFLGSEL
                     IARISHPPPMNELPFGAALERLTIRSQWGSGIQVARETGASYSGGAALTSAMEANEIG
                     QTYFGPVVLVVDAVTYSAGDIFAAGFQDHAIGPVLGTNGQTGGGGANVWPHDFLVRIL
                     PDVPEIKPLPGGAKLRVAARRCVRTGSFEGLPIEELGVRADELHRITEADVLEDNRDL
                     KAAAIALIADQPARWLEAELVTDKNGRAVRVTATGLDRVDIYVDDRPLASLDVEGGGP
                     AGTAVPKAWPAERVEVRGFVQNELKARRRLDLTPPPTS"
     gene            complement(10496..11206)
                     /locus_tag="P5558_05540"
     CDS             complement(10496..11206)
                     /locus_tag="P5558_05540"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HRY23803.1"
                     /translation="MDTVRSSNSRVLLAKIELTCGSLGCALDDLWSHPELPDRFPRFL
                     VLLHQIMRASVPLMELARDAALERRGSDPSAEGLAAYFAHHIDEERNHDVWTLEDLVA
                     AGFDGEAVLAQTPPPSLAAMVGSQYYWILHHHPAALLGYIAILEGNPPNQAHIDRLQA
                     QTGLPVAAFRTYRMHGRLDPHHRDELYRVIDELPLTPALAGLIGTSATHTAQMLAWSL
                     DGLDCIAHPPRTDERRAA"
     gene            11417..11608
                     /locus_tag="P5558_05545"
     CDS             11417..11608
                     /locus_tag="P5558_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23804.1"
                     /translation="MTKKSNLEQLVEAGVLDPANMGDEAKKIVNNEMTAEEVKHLIAA
                     HKRFPNNPPYKPDARGAGF"
     gene            11709..12836
                     /locus_tag="P5558_05550"
     CDS             11709..12836
                     /locus_tag="P5558_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRY23805.1"
                     /translation="MGAVPITRLADLTPLDRLGLPVWSATTPLARDLTTHLGKGTDAV
                     AARVSALMEAVERASAERVSLPLLRASWRELDGSGVAALDPRLCDLPPDSAFAPDAVL
                     GWIEADCLVGNRKLQLPLDLAVSPPGEGVLRHVDTNGLAAGNSRLEAIVHALAEVIER
                     DAVSQHLFAELYLDASDSLPQRLRLDPRTLPAPAGAIVETAREAGIDVVVDWLVTDIA
                     VATFRALMIDHAYPTPHGPATRCFVGHGTAPDAMLAVMRALTEAVQSRLAVIQGARDA
                     FNRAPAADHAARPFAWLADFTAQQLLPFDAVPTFESTDLAADLDFLLRQLARVGLDRV
                     IVADLTRPDLDIPVVRVRVPGLACFAVNQQRVGWRCRRLLL"
     gene            12844..14019
                     /locus_tag="P5558_05555"
     CDS             12844..14019
                     /locus_tag="P5558_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HRY23806.1"
                     /translation="MQRIAPAVVYLGPTLPREVVAAALPGADIRPPVRRGDLHRDRML
                     RYSLFILIDGVFFQDEAVSLREVVDILEDGAVVLGASSMGALRAAELWPAGMQGVGSI
                     YRLYRRSALVADDEVAVVFDPERPHPPMTVALVDVRWALRRAVRRGRLELHAAEALVA
                     RAERLPYAERTWVAIANGGLPPGLLGEIAGDSLKSRDALALAGRVRDLVQRDPTVLRR
                     PRRNTIPFESMDVRREALHDPAAWLDQGNRTHRFRRWLLASGRAGARAEPPIRTEKPD
                     DPLGENAEPVATPERPSTGDRLGATVVRFAALSDAIDVARAMNLKPEARHFLLAEAEL
                     VRAHGATDWWSLLAAPPVETDLLLEHRAELALAKSWRERLFRSSSAVGPGPGSGDAP"
     gene            14016..16679
                     /locus_tag="P5558_05560"
     CDS             14016..16679
                     /locus_tag="P5558_05560"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY23807.1"
                     /translation="MIALPIILPDGVVTMLFADIEGSTLLLQELGDAYAGVLGLYRSI
                     LRDAVDRHGGVEVDTSGDGYFASFPDAGNALEAAFDIQAALAEGAWPTGVAVRARIGL
                     HTGSVRVIDRTYLGLDVHRAARIAAAGHGGQIVLSKETLDACGPERFTGRFEFRDLGA
                     HRLKDLRYPELLLDAVRAGSNPRFAPLRTIDSRPTNLPQPATALIGRTDELDEIIDLL
                     GRGSVRLLTLTGPGGTGKTRLALEAAGALIDRFPAGVLHVPLAGIEDAELVVPAIAQT
                     LNIREQGETDLLVAIVNRLHRARMLLFLDNFEQVMGAAGAIVRLLESCPELQLLVTSR
                     ETLGSSFERVYPIEPLPLPTGRRANSFIMLAESPAVRLFVERVQDHDPGFELDATNVA
                     EIGEICRRLDGLPLAIELAASRLRLLSPSMLLGRLSQRLDILKGGRDKNARHRTLRAT
                     IDWSYRLLDENEQRLLQAVAVCAGGFSFQTAEAICQDLDWIDVDILDGITSLVGKSLV
                     ALSMVSGERRLRLLDTIREFAFEKLGESGRVVEMQDRHARHFVDLAEAVAPSLLSADQ
                     RARVGQLLDEQDNLRAALAWALQRPDAELSSRGITALLWFWISRGLFTEGGSWVAKAL
                     EHAEKAGAPHHRAAIVDAAGWLRMFSGDYAGALGHCRVAHELFAEHGTPTDVARTKMT
                     LGITLAVTGDEANGPPLIVEALEMFRAQGDDHGTVLALIALGEGLRAGGDNETAEHLY
                     NEALEKLQAQGNVYWPGQLLQNLGHMRLAAGDPAAAHAFFARALEYGQAYDYPIVTSL
                     CLAGFGGVALARGEPGIAARLLGKAQHRLDEMGAHFEPMDQAAFERYLADARERLGEA
                     ACAAAALEGRKWTLEEAFDAGRSLGDQDRDA"
     gene            16754..17638
                     /locus_tag="P5558_05565"
     CDS             16754..17638
                     /locus_tag="P5558_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRY23808.1"
                     /translation="MAKEIFCAFSIHCDAVAGWLGSYKGEDSPGDISRGVFAAEVGMP
                     RILDLFAHFDLKQSWFIPGHTIESFPDECAMVAKAGHEIGLHGYSHENPLAMSYEQES
                     AVFDRSIALIEELTGKRPTGYVAPWWEVSTNSVDILLKNGIKYDHSLMHRDFEPYYVR
                     VGDEWTKIDYAKPAETWMKPFTRGKATKLVEIPASWYLDDLPPMMFIKGFPNSHGYVS
                     PRHLGETWQETFDWVYRKHDYAVFAMTIHPDVSGRPQVLNMLEDLIAHMLKHPGVRFT
                     TLDTVADDFMRRTPPPKG"
     gene            17809..21009
                     /locus_tag="P5558_05570"
     CDS             17809..21009
                     /locus_tag="P5558_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23809.1"
                     /translation="MTGSDANTTTASVINLSERGWPRIGPNFPQPDNQIPVSLAGNDN
                     QPYNIDIKAGYGIEVFTVYYYIWDGSNAGQNDTLYTQPTGAFQMGAGDDTVTSDADGV
                     GEFPNPYFLLGEGNDHFDGSPGHLGSSTYGGGGDDVIIGGQNDDSLWGDEADSFLVDF
                     SYQPPYPSPYQFQLEPYDTANDGDDTISGGAGNDTIVGGNGNNKLDGGDGADTLTAGS
                     GSDYLYGGPRGDGFLDILTGGGGSDTFILSYGSDATGSSGDFWSGFFDAQTSAIGGNV
                     VSNLLASAFAKGVGDIAGAIAGPIGGAVLSDFVDFLFGLDSTPPPKPQTTDVMMVTDF
                     DPSMDVLILPLITNDQDGKDLTIEADFFAEFADGSTNAWGLKFSEDSTEFAEIRLADD
                     YVAALGYAPTDSEAPLQDILDALKVSAALIEPDGTFSTLASAEVLALLPSGGFTPPAN
                     AGIPSGATVDVFGAIGGQLTATGGVYTTIAGTQYSDALTVNAAIVDPATIRVSDLASQ
                     NSTVFGLGGGDVLYGGGASDTLVGGDGDDTLYSFLDALGSGESLSGGDGDDMLYGGAS
                     GGVFDGGAGTDTFGVVYGNLLKNALDQGPDGMQLVVDLTQGYAAERPASDDPDPGTAP
                     VGATPPFDDSVPNAYKLVDIENVIGGPLNDWIRAAQGSVIEGGAGPDYLDFGAGNVVL
                     SYASSNAGVNVNTSVQSPYAQGGDAAGDYYANLGSTTQSSLSALIGSANADSLYVLAS
                     VVNEAEALWINVTGLGGADVFGAFSNANPDNSEVTITDFSLADGDRIDLRPQGVTSFS
                     EGVTIAYAADTVIDINEASSSGLQIVLQGFDSTLSAGDFIFSQAVSGTAIGNHTDEGF
                     AGGPGDDVIEGRGGNDILYGNGGDDTLKGQAGQDWLYGGDGHDRLFGGAGNDAIDGGS
                     GDDLVRGGAGDDHIAGGAGDDTIRGDAGADTIDAGEGDDFVLGGLGDDILVGGLGDDV
                     ILGGAGVDTAVFTGAFSDHLVTGFGSLARTVEDLAGGGSRDRLADVELLQFDDGILDL
                     RSGAFDAGGVSSPAVEAPVAGPGFAADLEPLLTPVV"
     gene            21076..21522
                     /locus_tag="P5558_05575"
     CDS             21076..21522
                     /locus_tag="P5558_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY23810.1"
                     /translation="MIRQAIDADESAIRACAECAYARYVAAIGRKPAPMVADFAAQIR
                     AGQVHVAVDDAGLAQGFIVFFPKDGQMFLENVAVRPEAAGQGTGGALIRFCEDEARRA
                     GLAAVRLYTNARMTENLSLYPHLGYVEVERREEDGFDRVFFEKGLG"
     gene            complement(21533..22042)
                     /locus_tag="P5558_05580"
     CDS             complement(21533..22042)
                     /locus_tag="P5558_05580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY23811.1"
                     /translation="MTSRPFAVKPLEKEDRTSFTCGRDSLDHYFRTRIGQDVRRRIAT
                     AFIVLHKPTATVAGFYTLSAAQVPFTDLDEDWRRSLPRYPTLPAVLVGRLAVDRRFQG
                     QGLGGALLTDAASRAIRSDIGAHFLIVDALDEDAARFYQHHGMRHIADTDNRFFIPLS
                     VLAKAPSGH"
     gene            complement(22039..22311)
                     /locus_tag="P5558_05585"
     CDS             complement(22039..22311)
                     /locus_tag="P5558_05585"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRY23812.1"
                     /translation="MQKDARLEARVTPEVHALLKRAAEIEGRSMTDFVVAAASAAARQ
                     TIEQVEVIRLSRQGQQALAAALLDPPGIAPAMERAIASHRRLIATE"
     gene            complement(22367..23578)
                     /locus_tag="P5558_05590"
     CDS             complement(22367..23578)
                     /locus_tag="P5558_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23813.1"
                     /translation="MLETTFSAFTLGTQRLKNRVVMAPLTRQCAEPDGTPTAEMAAYY
                     ARRARGGVSMIITEGTWQNDELGCVAYLGQPGCLTKRHIEGWRRVTDAVHAHGIPIVL
                     QLMHGGRVSDPRCLHEGEQPVSASAARSPGWVLYTDTDQEKHDRLIEGAWPKVGFPPA
                     RALTEAEMERIADGFAEGAARGVEAGFDGVELHGANGYLLYQFIDPKLNRRTDAWGGS
                     AENNVRLAKLACERTRAAIGPDKLITLRLSQDGVDDFTGAWQGGVAYAREVGAALADC
                     AADALHWSSFAWSDNRDPSDPTPMPRALREASGKPVIVNGGIAEGSDAEEVLTSGAGD
                     LCAIGRPIFAHPDWPHIIRSGEPYDWTPFDRKYVIDPPRDHALGYPCNLEAPDWDPDL
                     SKRRREGWMPS"
BASE COUNT         3528 a         7910 c         8590 g         3643 t
ORIGIN      
        1 aggcgcgagc ttcctctatc tcgggcggaa ctaccgcctg cgcttcgtct ctgacacgag
       61 tcggccgctg gtgctgcgga acggctggtt cgagctcgac gagcgcgtca cggccgaagg
      121 cggagcgcct gcggcgcgca aagtcttccg ggacttctac acgacgcgag ccgcgtccct
      181 gctcgaggaa aggatcgctc tcttcgcgcg ccgcgtcgac gtccggcacg ggcccgttgc
      241 ggtccgcgag ctcggctacc actgggcctc atgcggaccg agcggcagcc tgaacttcca
      301 ttggaagatc atgatggcac cctcgacggt catcgactac gtcgtcgcgc acgagctctg
      361 ccacttgaaa cacagggacc actcggtggc cttttggaat gaggtcgaca aggtgctccc
      421 gagctggcgg gacaagaagg actggttgcg ccgatgcggt gccgcgctcg acttgtagct
      481 gggctaaagc tgcaggccgc cgctcgaatg aaacggccca aagcactgag ggcattgtgt
      541 aggatcaatc tgaagcccgg tgacttttcg acgccagttc cctgcggaaa gcgaacgaaa
      601 tcaggaaccc gcgagctttg gcacctcgaa ctatccaaac tatctcagat agatagagct
      661 gttgaccgaa atccccgtgg tcaacagctt cggagaaacc gggctctcag agaacgcctt
      721 cgggtctgcc aagcctccgg ccagtgaaca gccccgcccg tataaccctc gaaaacaacg
      781 gaaaaatccg gctgcggctc agatggggag acgcatttcg cgggggcgac tggcggaggg
      841 ggtgggattc gaacccacgg tacgctcgcg cgcacaacgg ttttcgagac cgccccgatc
      901 gaccactccg gcacccctcc agcgcgggcg catgtttagg gaaacctcgg ggccgatgca
      961 aggtgcagcg atggcatggt gcttgacagt gcatgcgcgg ggcggaactg tccgcgcctg
     1021 ccatcgagca acccgatcgg agcccgacca tgcgtccctg caccccggcc ttgcccttcg
     1081 ccctgctctc cctgctggtg gccacggcgc cgctggcggc ggctgacgag atccgcgagc
     1141 agctcgacct cgccatcgag ctctacgagg aaggcgacct cgccgggagc atcaccgagc
     1201 tgcaatatgc catcggcgag atccagggtc ggctcggcag cgcctatgcc gagaccttcc
     1261 cgccggcgcc ggcgggctgg acggccgagg cgccgagcca ggaggcttcg cccgccttcc
     1321 tcggcggcgg cacgatggtc agccgcgcct atcgcgagga cggcggctcg ggcacgatga
     1381 atgcccagct catgatcgac aacccgatga tccagggcat ggcggcgctc atgagcaacc
     1441 cggcgatcat ctcggccaat ccgaacatga agcgcatccg gctcggccgc gagaatgccg
     1501 tcctcgattt cgaccaggct gccgggcgcg gcgagatcac gttcctgatg ggcggcggcc
     1561 gggccatgct gaaggtcgag ggctccggcc tcgcctcggg cgatctcctc gtcgagaccc
     1621 tcgaggcctg ggaccggcag gggctcgagg atgtggccgg cctctgaccc ggggcgatga
     1681 tcgacgatcc ggccttctat ctggccgcca tcccggcggt cctgatcttc ggcatctcca
     1741 agggcggctt cggcggcggc ctcggcatcg ccgccgtgcc gctgatggcc atcgtcgtct
     1801 cgcccgcccg tgccgccggc atccttttgc cgctgctcgt gctcatggac ctgatcgggc
     1861 tcttcgccta tcgccgccac tgggaccggc gggtcgtcaa ggtcatgctg ccgggagcgc
     1921 tgctcggcat cctcatcggc ggcctcgcct tcggctatct cgacgacgac gtggtccggg
     1981 tcgtcctcgg cctgatcgcg gtcggcttca cggccgacta tttcctccgc gacaagagcc
     2041 ggatcgcggc ccggccgcag cgccgggtgg ccggcgcctt ctggggttgc gtcgccggct
     2101 tcaccagcac catcgcccat gccggcggtc cgccggcgaa catctacctc ctgccgctca
     2161 agctcgacaa atccatcttc gtcggcacga tggtcgtgct cttctccgcc gtcaacgcgg
     2221 cgaagatcgt gccctacgcg tcgctcggcc tgttcgatgc cgccaacctc accacctcgc
     2281 tcgtgctcgc gccggtggcc gccatcggca tgctcgccgg catctggctg cacggcaagg
     2341 tcgaccagct cctcttctac cggctctgct acgtgttcgt cctgctgacc ggcctcaagc
     2401 tggtctatga cggcctcacc tgatggaagt gtaggaatta tagccggata tggcgcggaa
     2461 cgcaacccgc tggcgggggt ttggggctcg ttcggcatat tcggcttttc agccggcccg
     2521 cggcgggaac aatgtcggcg gcagggcaaa aaaagcaatc ccgacagcac gttggctggc
     2581 ctcgggtttg gtcgtgaaat gccgggttca ttcgttgatt cctcgggctc gttcgtcggt
     2641 gcgtgtcgcg gattctgcga cagtcgcggc gttcatgttg cacgagaggc gacaacccga
     2701 gccgagctgc cgcagccgat gcgacagccg ggccaaagcg gccggcaggg ctcccgtgac
     2761 gacgccgacc ggcgtccgcg ccgcgtcggg catgagcatt catgagcatt gatgatgtca
     2821 cgtcggtcac cgagtggcga ggcgggcacg aagccactag gctcgccaga gcctaccggg
     2881 ttggtcgcag ggttcgaggt ggcagcaaaa ggttaccaga gcttggtgga gacgatcgtc
     2941 ggcgaggatt tctcgctggt acaaagtctc ttcctgtcgg cccaggcgcg gccgccggtc
     3001 gtggtgtgga cgccgcgccc ggtggtgctg cgttcgcccc tgctcgagcg cctgctcgac
     3061 agatggtcgg tgctggccgt ggggtcgcac ccgccatcac ccgacaaggc ggcggccttc
     3121 atcgggtgcg agctcgcgga ctggacgatg cagctcgacc tcgagcagcg ggcggacgat
     3181 gatcccgggc ccggggcctt cgtctatcga cactatggcg ccggcatcgc ccggcagtat
     3241 ggccgggaca tgaccggctt gcggccggat gccttcgccg gccatatcgg caggttcttc
     3301 tcggcggtct atgacgcggt catgcgccgt cccgagcccg tcttcaccga gcacgagccg
     3361 ccggccgccg tgctggtgcg cagctggcgc cggctggtca tgcccttctg cgatccgcgc
     3421 gggcaggtcg ttcgcttcgt cgtcggcaac attcccgaaa gcccggtcgg cacgctgctc
     3481 gacgtcatgc acgatgcagc cctcgtgacc gacgggcagg gccgtgtccg gctcgccaac
     3541 atcgcggcga gcacgctcct cgggacaccg tccgggcgaa tcgtcggctg ctcgatcgac
     3601 gagctcgtgc ccgagctccg gccgctgatc gccggggcca gtggtgagat cgagggggtc
     3661 agctctttcg gtcgccggga gatcggcttc gtccgggacg gccggccgct ggtgctcgac
     3721 gtctcgatcg gcgggacgcg cgtcgccggc gagccgctct tcgtcctggt gctgcgcgac
     3781 cagaccgagc gggtggcgcg cgagcgctcg ctcgccctgg aggtgacccg cgacccgctc
     3841 accggcatcc tcaaccgccg cggctttcac gagcgcctgg cgccgctcct gctgccgcgg
     3901 cggcgccggt cggacggctt cgccctcttg gtcatcgaca tcgacgggtt caagctcctg
     3961 aacgaccgcc atggccatgc ggcgggcgac aaggtgctgc aggccacggc cgagcggatc
     4021 tcgcaggcgc tgcgcggcga cgatctgttc gcccgctggg gcggggacga gttcgtgctg
     4081 gcgctcaatg gcgtcaccga gccggcggcg ctgatggccg ctgccgacaa gatccgccgg
     4141 gcgctcgatc ccgagctgac ggcggagcgg ccggtcgggc tgagcatggg cggcgccttc
     4201 catccgctgc acggccggga cttcgagagc gtcttcgcgg cggccgacaa ggcgctctac
     4261 gcggcgaagg cgcgcggcgg gaaatgcctg gagattgcgg taggctgagt tgggccaggc
     4321 gaggcgtcaa cgcagcaggt cgcgctccgc gtccagatca tcgagggtga gggcaggttc
     4381 attcgattgg cggtgggcca gcagcgccgc ctcggcggtg aagcgatcct cgaggtcctc
     4441 gaggtgctcc gccaccgcct cgcggacgtg atctgcctgc tcatggcgat ggttctcttg
     4501 ggacatgggc attttggacg gcgctaccgc gtcacgccga aagagctcgc tgcacggcgg
     4561 cgggttcctt ctcgatcacc cgcaggaggg tcgccgccgg gccgctgacg cgtcgctggc
     4621 cctgctccca tttgcgatag cccgagaggc tcatgcccat cagcggggcc atctgtgcct
     4681 gggtcagctt cgcccgggtc ctgatctcgc gcggatcgac cggctcatgc gtgatcgcgg
     4741 ggccggttcc cttggcatga gcgagagctt cgcccatcgc ctcgatcagg tcttcaccga
     4801 aggtggtcat tttctctttt tccatgccgc tttcagcgcg gcggcaagtc ccgccaccac
     4861 ccgagccgca gatgtgtata gcccagtggg tcatctttcg aaatctcgtc aggcgcgccg
     4921 tttgcggacc aggccgaggc gggcgcgggc ctttgcgggg tcggggggga ggacggcggc
     4981 gaggaggttc ctcgtgtagg ggtggctcgg ggcgtcgagg acctggtcgc gggggccctc
     5041 ctcgacgacc ttgccggcct gcatgacggc gatgcggtcg gcgaggtggg cgacgacggc
     5101 gaggtcgtgg ctgatgaaga ggagggcgag gccatggcgg cgctgcagct cggccaggag
     5161 gttgagcacc tgggcctgga cggagacgtc gagggcggag accggctcgt cggcgaccag
     5221 gagggccggg cgggtgatga gggcgcgggc gatggcgatg cgctggcgct ggccgccgga
     5281 gaactcgtgc gggtagcggt cgagcgcctc ggcgccgagg cccacctcgg ccagcgcctc
     5341 acggcaccgc tcggcggccg cggcgcgacc gaggtggagc aggccgagcg gctcggcgac
     5401 gatttgggcg atcttctggc gcgggtcgag cgagccgtag gggtcctgga agaccatctg
     5461 ggcgtcccgt cgcagccgcc gcttccccgg tcggccggtg gcccagagat cggcgtcctg
     5521 gaaccggacc gtgccgctgt cgggccgctc gaggcccacg gcgaggcggc cgagggtcga
     5581 cttgccggag ccggattcgc cgacgatgcc gagggtctcg ccggcccaaa ggcggagcgt
     5641 gacgccgtcc agggcgacgc gcctcggtgc cggcccgagg agccgctggc ggggcaggcg
     5701 gtagagccgg cggacatcgg tgagctcgag gaggcaatcg gaggcggggt caggcacggt
     5761 cccgcccccg tgccaggggg tggaagcagg ccgcccggtg cgccggtgct tcgagggggg
     5821 cgaggaccgg ccggaccgaa tggcagaggg cgtcggcgcg gtggcagcgc ggcgcgaagc
     5881 tgcaggcctg cgcggcaccg ccgggctccg gcacctgccc gggaatggcc gcgagccggc
     5941 cccgcccggc ctcgccgagc gctgttggca gggcatcgaa gagaccgcgc agataggggt
     6001 gggcgagttc gtcgaagacc aggtcggtcg ggccggtctc gacgatctgc ccggcataca
     6061 tgacgtgcac ggcctcggcg atggccgcca ccacgccgag gtcgtgggtg atgaagagga
     6121 gcgccatgcc ggtctcgtcg gcgatctcgg cgatgagctt caggatctcc gcctgcacgg
     6181 tgacgtcgag ggccgtggtc ggctcgtcgg cgatgagcag cttggggcgg cgggccagca
     6241 ccatggcgat catcgcccgc tggcgctgcc cgcccgagag ctggtgcggg tagagcttgg
     6301 gcgagaactg ttccgggtcg agcccgaccc ggcgcatggc gtcgcgcgtg cgcgccgcga
     6361 tgtcggcccg gccgagccgg ccgtcgaggc gaaggccctc ggcgatctgc cagccgatgg
     6421 tgcggaccgg gttgagggcg gtcatcggct cctggaagat catgccgatc tcggcaccgc
     6481 gcagccggcc gagcgccggc tcgtcgagcc cgaggagatc ccggccgtcg aagacgaggc
     6541 ggccgccgag ccgggtgcgg tccggctgga ggcgcatgat cgagagggcg gtcagcgact
     6601 tgccgcagcc gctctcgccg acgatgccgt gccagtcgcc cgcgttcagg gcgaggtcga
     6661 tgccgtcgag caggcggagc gtgcccgccg gcgtcggcag gtcgagggtc aggccttcga
     6721 tctcgaggag ggtcactggc gcagccgcct gagcctgggg tcgaggaggt cgcgcaggcc
     6781 gtcgccgagg aggttgaggc cgagcaccga aagcgcgatg cagagacccg gcaggatggc
     6841 gagctgcggc gcgatgaaga ggaaagtctg cgcctcgttc agcatccggc cccaggaggg
     6901 gtctggcggc tgggcgccga atcccagata ggagagcccg gcctcggcga ggatggcgag
     6961 ggcgaactgc accgtcacct ggacgatgac gaggctcgag aggttcggga tgatgtgcga
     7021 ggtggcgatg gcgagctcgc cgcggccggc gaggcgggcg gcggcgacga agtcgcgacg
     7081 ccagaggccg agggcggcgc ctcgggtcag ccgggcgaag accggcacgt tgaagatgcc
     7141 gatggcgagg atggcgttga cggcacccgg gccccagatc gcggtgatca tcacggccgt
     7201 tagcagggca gggaaggcga aggcgaagtc ggagaggcgc atgaccagct cgtccagcca
     7261 gccgccggcg gcgctcgcca gcgcgccgag cggcacgccg aggccgacgc cgatcgtgac
     7321 cgccaccacg gcgacgagga tggagtttcg ggcgccggcg aggagcagcg agaggatgtc
     7381 gcggccatag ggatcggtgc cgagccagtg gtccggcgag gcgccggcga ggcggttggc
     7441 gacgtcgatc acgtcgaccg ggtagggggt ccagacgagc gacaagaggg cggcggcgag
     7501 gagcaggagg gagagcgcgc cgccgatcca gaggtgatgg tgcctagtca tggcggagcc
     7561 gcggatcgat cagcacgtag aggacgtcga ccacgaagtt gacgaggatg acaacggcgg
     7621 cgagcacgac gacgaggtcg cggacggtgg tgaggtcgcg ctgggcgatc gcctggaagg
     7681 tcagtcggcc gatgcccggg aggtagaaga cgttctcgat gatgatggtg ccggcgagca
     7741 ggaaggagaa ctgcaggccc atgatggtga cgaccgggat gagcgcgttg ggcaccgcat
     7801 gctgccagat cgcccggccg cggctcaggc ccttggcccg ggcggtccgg acgtagtcct
     7861 cggccagcgc gtccagcacg gccgagcggg tgaggcgggc gaggatcgcc gcctgcggca
     7921 gggcgagggc cagggccggc atgagcagcg agggcatcgc ctgccagaag cccgcctccc
     7981 agcccgggaa gccgccggcc ggcagccagc cgaggccgac ggcgaagagc aggatgagca
     8041 ggatggcgaa ccagaaattg ggaatggcga cgccgagctg ggtgacgctc atgagcagcg
     8101 tgtcggcgag gcggccgcgc cggctcgcgg catgcacgcc cacggggatg gcgatggcgg
     8161 tcgagagcag gatcgaggcg agggcgagcg gcacgctgac ggtggcccgc tcggcgatca
     8221 gctcggccac cggcacgccg taggtgtagc tctggccgag ccggccctgg acgagcccgc
     8281 cgagccaggc gaggtagcgc tccaacggag gacgatcgag cccgagctgg gcgcggagtg
     8341 ccgccagcgt gtcctcgcgc gcgccggtgc cgaggatgac ggcggccggg tcgcccggca
     8401 gcagctccag cacgacgaaa accaccaccg tcgccgcgag gagggtcagc agcagcgtca
     8461 ggaggcggcg gaggatccag ggcgccaagg gcgcatcccg tcgaaggtcc tcatgacgtc
     8521 ggtggcggcg tgaggtcgag ccggcggcgc gccttgagct cgttctggac gaagccccgg
     8581 acctccactc tctccgccgg ccacgccttc ggcaccgcgg tgccggccgg cccgccgccc
     8641 tcgacgtcga gcgaggccag cggccggtcg tcgacgtaga tgtcgacgcg gtcgaggccg
     8701 gtcgccgtga ccctgacggc gcgcccgttc ttgtcggtca cgagctcggc ctcgagccag
     8761 cgggcgggct ggtcggcgat cagcgcgatg gcggcggcct tgaggtcgcg gttgtcctcc
     8821 agcacgtcgg cctcggtgat ccggtgcagc tcgtcggcac gaacaccgag ctcctcgatg
     8881 ggcaggccct cgaagctccc cgtgcggacg cagcggcgcg cggcgacgcg cagcttggca
     8941 ccgccgggca ggggcttgat ctccggcacg tcgggcagga tccgcacgag gaagtcgtgc
     9001 ggccagacat tggcaccgcc accgccggtc tggccgttcg tgccgagcac cgggccgatg
     9061 gcgtggtcct ggaaaccggc ggcgaagatg tcgccggcgc tgtaggtgac cgcatcgacg
     9121 accagcacga ccgggccgaa ataggtctgg ccgatttcat tggcttccat ggcggaggtc
     9181 agcgcggcac cgcccgagta ggaggccccg gtctcgcgcg cgacctggat gccggagccc
     9241 cactggctcc tgatcgtcag gcgctcgagg gcggcaccga acggcagctc gttcatgggt
     9301 ggcgggtggc tgatcctggc gatcagctcg gagccgagga actggaaggg caaaggcgtg
     9361 atccgccggt gcgtcaggaa ctgcaggatg ccttcggcgc aatcgacgat gccgccggga
     9421 ttgccgcgga tgtcgatcac cagcccgcgc ctgggcagga gcccgagcag gcggcggaac
     9481 tcgtccttga actcggccac gtcgtcatgc tcgaaggcgc gcagccgcag atagccgtaa
     9541 ggctcgccgc ccttgggatc ggccaggcgc tcggcggtca tcaggtcggg caggtgcgag
     9601 gcgaggtcgt ccggggctcg ccgcctcgcc ggcgccttct tctgctcacc ggcgaagagg
     9661 tgccggcgcg tcacctgctc gacctgggtg cggatgttcg tggcgtggtt gagcgagtgc
     9721 ggcggctgcg gctcgccgtc gctcgtgtcc ttctcgaaca gccagaccgt ctggatggcg
     9781 aagcgcaggc ccgggtcgtt gagctcgagc atcgaccagg ggaggcggat ctcggcctcg
     9841 ccggacggat ggcgatagcc gaggatcacc cagtcctcgt cgggcacgag gcaggcgccg
     9901 agccagcgca cggtcatgaa gaggcaaccc agggcggcct gcgattccgg gttggagccc
     9961 tcctcgcggg cggcgttgat ggcgatcgcc cgggcgatcg gggcgccgtg ccagtgggtg
    10021 atctcgacgc ccggccggaa cgtcggatgg tcgaagtggg cgaggacgcg ggtgacgagg
    10081 tagcgggtgg tgccgtgggc gtccgtgcag ggctcgagga ggaagggcag gaaggcggtc
    10141 tgctcggcga agggtggcgg ggcggtgtag gccgtgtgga tgtcgcgcag gctcttgaag
    10201 atgcgcagca tctcgctgtg gaaccgcaga tcgccgagcc cggccgcctg gtcgcgcagc
    10261 acgcggagcg cctgcgccgg atcgatggca tggcgcaccc gcttcatgtg caggtgggtg
    10321 tagagctgct cgatgatgat cagcgcctgg tcgacgatca gcacccggtc ggcgccggtg
    10381 agcttctgcc gcatggaggc gagctcgccg accggcctga ccttgccgag cttcccgaac
    10441 gccttgccga gcgcctcgat tgtggccggg tcggcgccga cgggcgtcac cgtggtcatg
    10501 cggcccgccg ctcgtcggtg cggggcggat gggcgatgca gtcgagcccg tcgaggctcc
    10561 aggccagcat ctgcgccgtg tgggtggcgc tggtgccgat cagcccggcg agcgctggcg
    10621 tcagtggcag ctcgtcgatg acccggtaga gctcgtcgcg atggtgcgga tcgagccgtc
    10681 cgtgcatgcg gtaggtgcga aaggccgcca cgggcaggcc ggtctgggcc tgcagccggt
    10741 cgatatgcgc ctggttcggc gggttgccct cgaggatggc gatgtagccg agcagggctg
    10801 ccggatgatg atgcaggatc cagtagtatt gcgagccgac catggcggcg agcgagggcg
    10861 gcggcgtctg ggcgagcacc gcctcgccgt cgaagccggc ggccaccagg tcctcgagcg
    10921 tccagacgtc gtgatttcgc tcctcgtcga tgtggtgggc gaagtaggcg gcaagccctt
    10981 cggccgaggg gtcgctgccg cggcgttcca gggcggcgtc cctggcgagc tccatcagcg
    11041 gcacgctggc ccgcatgatc tggtggagga ggacgagaaa gcgcgggaag cggtcgggca
    11101 gctcggggtg cgaccagaga tcgtcgagcg cgcagccgag gctgccgcag gtgagctcga
    11161 tcttggcgag gagcaccctg ctgttgctcg accgaaccgt gtccatgccg ccttctcccc
    11221 agctcgtgcg ttcttgtccg ggcattgcca ggcccgcgaa tcagagcacg gcggccggtc
    11281 gcgcgcaacc tgcagcacac tggtcagagc gctcgccgcg caatcgacca tggcctcgac
    11341 caacgcggga gtgtataact gcgcgcgctt gcggtgacat cgacttgcag gcgaaaccaa
    11401 gagggaacgc gaccagatga ccaagaagtc gaacctcgag cagttggtgg aagcgggtgt
    11461 gctcgacccg gccaacatgg gtgacgaagc caagaagatc gtgaacaacg agatgaccgc
    11521 cgaggaggtg aagcatctca ttgccgcgca caagcgcttc ccgaacaacc cgccctacaa
    11581 gccggacgcc cgcggcgccg gattctgatg cgccagccgg aaattcgact ccggcgacgc
    11641 gcaagcacga cggcctcacc ccgcgggtcc ggcctcccgc ctggacggag gggcggctgc
    11701 gcgccctgat gggtgccgtg ccgatcaccc ggctcgccga cctgacccca ctcgaccggc
    11761 tgggcctgcc ggtctggtcg gcgacaacgc ccctggcgcg cgacctgacg acccacctgg
    11821 gcaaggggac cgatgcggtc gccgcccggg tgagcgcgtt gatggaggcg gtcgagcggg
    11881 cctccgcgga gcgcgtctcc ttgcccctgc tgcgggctag ctggcgcgag ctcgacggat
    11941 cgggcgtcgc ggccctcgac ccgcggctct gcgacctgcc gcccgacagc gcctttgcgc
    12001 ccgatgcggt gctcggctgg atcgaagccg actgcctcgt cggcaaccgg aagctccagt
    12061 tgccgctcga tctcgcggtc tcgccgcccg gcgagggcgt tctccggcac gtcgacacca
    12121 acggcctcgc cgccggcaac agccggctgg aggcgatcgt gcatgcgctg gcggaggtga
    12181 tcgagcgcga tgcggtgagc cagcatctct tcgccgagct ctatctcgat gcgtcggaca
    12241 gcctgccgca gcggctgcgc ctcgaccccc ggaccctgcc ggcgccggcc ggtgcgatcg
    12301 tcgagacggc gcgcgaggcg ggcatcgacg tcgtggtcga ctggctggtg accgacatcg
    12361 ccgtcgccac cttcagggca ctcatgatcg accacgccta tccgacgccg cacgggcccg
    12421 ccacgcgctg cttcgtcggc cacggcacgg cgccggacgc catgctggcg gtgatgcggg
    12481 cgctgaccga ggcggtccag tcgcggctcg ccgtcatcca gggcgcccgc gacgccttca
    12541 accgggcgcc cgcggcggac catgccgccc gacccttcgc ctggctcgcg gatttcaccg
    12601 cccagcagct cctgccgttc gacgctgtcc ccaccttcga gtcgaccgat ctcgcggccg
    12661 atctcgattt cctgctgcgg cagctcgccc gggtcgggct cgatcgcgtg atcgtcgccg
    12721 atctcacccg ccccgatctc gacattccgg tggtgcgggt gcgcgttccc ggcctcgcct
    12781 gcttcgccgt caaccagcag cgcgtcggct ggcgctgccg gcggctcctg ctttgaggcg
    12841 ctgatgcagc gcatcgcccc cgccgtcgtc tatctgggcc cgaccctgcc gcgcgaggtg
    12901 gtggccgcgg cgttgcccgg tgccgacatc cgcccgccgg tgcggcgcgg cgacctgcac
    12961 cgcgaccgga tgctccgcta ctcgctcttc atcctgatcg acggggtttt tttccaggac
    13021 gaggccgtgt cgctgcgcga ggtcgtcgac atcctcgagg atggcgccgt cgtgctgggt
    13081 gcctcgagca tgggtgcgct tcgggcggcc gagctctggc ccgccggcat gcagggggtg
    13141 ggcagcatct accgcctcta ccgccggagt gccctggtgg ccgatgacga ggtcgccgtc
    13201 gtcttcgatc cggaacggcc gcatccgccc atgaccgtgg ccctggtcga cgtgcgctgg
    13261 gcgctccggc gggcggtgcg ccgcggccgg ctcgagcttc atgcggccga ggcactcgtc
    13321 gcccgggccg agcgcctgcc ctatgcagaa cgtacctggg tggcgatcgc caatggcggg
    13381 ctgccgccgg gcctgctcgg cgagatcgcc ggcgacagcc tcaagtcgcg cgatgcgctg
    13441 gcgctcgcgg gccgggtgcg cgacctcgtg caacgggacc cgacggtcct ccggcggccg
    13501 cggcgcaaca cgataccgtt cgaaagtatg gacgtgaggc gcgaagcgtt gcacgatccg
    13561 gcggcgtggc tcgatcaggg caatcggacg catcggtttc gccgctggct cctggcgtcg
    13621 ggccgtgcgg gcgctcgggc cgagccgcca ataagaacgg aaaagcccga tgaccccctt
    13681 ggcgagaatg cggaaccggt ggccacgccg gagcgcccga gcaccggcga tcggctgggt
    13741 gcgacagtcg tgcggttcgc ggcgctcagc gacgccatag acgtcgcccg cgccatgaac
    13801 ctgaagcccg aggcccggca cttccttctg gcggaggccg agctcgtccg cgcgcatggt
    13861 gctaccgact ggtggtcgct gctcgcggcg ccgccggtcg agaccgacct gctcctcgag
    13921 catcgagcgg agctggcgct cgccaagtcc tggcgcgagc gcctgtttcg ctcgtcatcg
    13981 gcagtcgggc ccggcccggg ctcgggcgac gcgccatgat cgccctgccg atcatcctgc
    14041 cggatggcgt cgtgaccatg ctgttcgccg acatcgaggg gtcgaccctg ctcctccagg
    14101 agctgggcga cgcctatgcc ggcgtgctcg ggctctatcg ctcgatcctg cgcgacgccg
    14161 tcgaccggca cggcggggtc gaggtcgaca cgtccggcga cggctacttc gcctccttcc
    14221 cggatgccgg caacgccctc gaggcggcgt tcgatatcca ggccgccctc gccgagggtg
    14281 cgtggccgac gggggtggcc gtgcgcgccc ggatcggcct ccacaccggc agtgtgcggg
    14341 tcatcgaccg gacctatctc ggcctcgacg tccatcgcgc ggcgcggatc gccgccgccg
    14401 ggcatggcgg ccagatcgtg ctgtcgaagg aaacgctcga tgcctgcggc cccgagcggt
    14461 tcacgggacg cttcgaattc cgcgacctcg gtgcccacag gctcaaggat ctccgctatc
    14521 ccgagctgct gctcgacgcg gtgagggccg gcagcaaccc gcgcttcgcg cccctgcgca
    14581 ccatcgacag ccggccgacc aacctgccgc agccggccac ggcgctcatc ggccggaccg
    14641 acgagctcga cgagatcatc gacctgctcg gtcgcggcag cgtgcgcctc ctgacgctca
    14701 ccggccccgg tggcaccggc aagacgcggc tggcgctgga ggcggcgggt gccttgatcg
    14761 accggttccc ggcgggcgtg ctgcacgtgc cgctcgccgg catcgaggat gcggagctgg
    14821 tcgtcccggc catcgcccag accctgaaca ttcgcgagca gggcgagacc gatctcctgg
    14881 tggcgatcgt caaccgtctg catcgcgcca gaatgctgtt gttcctcgat aacttcgagc
    14941 aggtcatggg tgccgccggg gccatcgtcc ggcttctcga gagctgtccg gagctgcagc
    15001 tcctggtgac cagccgcgag accctgggct cgtcgttcga gcgggtctat ccgatcgagc
    15061 ccttgccgct gccgaccggg cggcgggcca actcgttcat catgctggcc gagagcccgg
    15121 ccgtgcggct cttcgtcgag cgggtccagg accacgatcc gggcttcgag ctcgatgcga
    15181 cgaacgtggc ggagatcggc gagatctgtc gccggctcga cgggctgccg ctggcgatcg
    15241 aactcgcggc gtcgcggctc aggctcttgt cgccgagcat gctgctcggc cggctgtcgc
    15301 agcgcctcga catcctcaag ggtggccggg acaagaacgc ccggcaccgg acgctgcggg
    15361 cgaccatcga ctggagctat cgcctgctcg acgagaacga gcagcggctg ctgcaggcgg
    15421 tggcggtatg cgccgggggc ttctccttcc agacggccga ggccatctgc caggatctcg
    15481 actggatcga tgtcgacatc ctcgatggca tcacctcgct cgtcggcaag agcctcgtcg
    15541 ccctttccat ggtgagcggc gagcgtcgcc tgcgcctcct cgacacgatc cgcgagttcg
    15601 cgttcgagaa gctgggcgag agcgggcgtg tcgtcgagat gcaggatcgg catgcgcggc
    15661 acttcgtcga tctggcggag gccgtggccc cctcgctcct cagcgccgac cagcgcgccc
    15721 gggtcggcca gttgctcgac gagcaggaca acctgcgcgc ggcgctcgcc tgggcgctgc
    15781 agcggccgga tgcggagctg agcagccgcg gcatcaccgc gctcttgtgg ttctggatct
    15841 cgcgcggcct cttcaccgaa ggcggaagct gggtggcgaa ggccctggag cacgccgaga
    15901 aggctggcgc gccgcaccac agggcggcaa tcgtcgacgc cgccggttgg ctgcgcatgt
    15961 tttccggcga ctatgccggg gcgctcgggc actgccgggt cgcgcatgag ctctttgccg
    16021 agcacggcac gccgaccgac gtcgcccgca ccaagatgac gctcggcatc acgctcgccg
    16081 tcaccggcga cgaggcgaac gggccgccct tgatcgtcga ggcgctggag atgttccgcg
    16141 cccagggcga cgatcatggc acggtgctgg cgctgatcgc gctcggcgag ggcctgcgtg
    16201 ccggcggcga caacgagacc gcggagcatc tctacaacga ggcgctcgag aagctgcagg
    16261 cacagggcaa tgtctactgg ccgggccagc tcctgcagaa tctcgggcac atgcggctcg
    16321 cggcaggcga tccggcggcg gcgcatgcgt tcttcgcccg agccctggaa tacggtcagg
    16381 cctacgacta cccgatcgtc acgagcctct gcctcgccgg gttcggtggc gtggcgctgg
    16441 cgcgtggcga gcccgggatc gcggccaggc tgctcggcaa ggcgcagcac cggctcgacg
    16501 agatgggcgc gcatttcgag cccatggacc aggccgcctt cgagcgctac ctggccgatg
    16561 ccagggaaag gctcggcgag gcggcctgcg ccgccgccgc gctcgagggt cgcaagtgga
    16621 cgctggagga ggcgttcgat gccggccggt ccctcggcga ccaggatcga gacgcataac
    16681 gttcgacgcc gctcttgcgc gaagccctcg ctcatggaac gctgcccggc gagcaacgat
    16741 ggggagcgcc gccatggcca aggaaatctt ctgcgccttc agcattcatt gtgacgccgt
    16801 cgccggctgg ctcggctcgt acaaggggga ggacagcccg ggcgacatct cgcgcggcgt
    16861 cttcgccgcc gaggtcggca tgccgcgcat cctcgatctc ttcgcgcatt tcgacctgaa
    16921 gcagtcctgg ttcatccccg gccacacgat cgaatccttc cccgacgagt gcgccatggt
    16981 ggcgaaggcg gggcacgaga tcggcctgca cggctacagc cacgagaatc cgctggccat
    17041 gagctacgag caggaatcgg ccgtcttcga ccgctccatc gcgctcatcg aagagctcac
    17101 cggcaagcgg ccgacgggct atgtcgcacc ctggtgggag gtctcgacca actcggtcga
    17161 tatcctcctg aagaacggca tcaagtacga ccacagcctg atgcaccggg acttcgagcc
    17221 gtactacgtg cgggtcggcg acgagtggac caagatcgac tacgcgaagc ccgccgagac
    17281 ctggatgaag ccgttcaccc gcggcaaggc gacgaagctc gtcgagatcc cggcctcctg
    17341 gtatctcgac gacctgccgc cgatgatgtt catcaagggc tttcccaaca gccacggcta
    17401 tgtcagcccg cgccatctgg gcgagacctg gcaggagacg ttcgactggg tctacaggaa
    17461 gcacgactac gccgtcttcg cgatgaccat ccaccccgac gtctccggcc ggccgcaggt
    17521 cctgaacatg ctggaggacc tgatcgccca catgctgaag catccgggcg tgcgcttcac
    17581 gacgctcgac acggtcgccg acgacttcat gcgaaggacg ccgccgccca aggggtgagg
    17641 gggccgccgg gccgtcgcgt cgcccgtcgt gcatgggcgg cctaggaacg gccgaggcgt
    17701 gggcagtggc ccggcgggtc gccgcaacgc actgcattga caacaggaat caggttcgta
    17761 tgcaatccgg tggctcttct ccatgccacc gacccgaagg ccacagagat gactggctcg
    17821 gacgcgaaca ccaccaccgc ctccgtcatc aacctctcgg agcgcggctg gccgaggatc
    17881 ggtcccaatt ttccgcagcc ggacaatcag ataccggtct cgctggccgg caacgacaac
    17941 cagccgtaca atatcgacat caaagccggc tacggcatag aggtcttcac cgtctactac
    18001 tatatctggg acggttcgaa cgccgggcag aacgacacgc tgtacacgca gccgacgggc
    18061 gccttccaga tgggggcggg cgacgacacg gtcacgagtg atgccgacgg agtcggcgag
    18121 ttccccaatc cttattttct cctgggcgaa ggcaacgatc acttcgacgg ttcgccgggt
    18181 catctgggat ccagcaccta tggcggcggg ggtgacgacg tcatcatcgg cggccagaac
    18241 gacgacagcc tctggggcga cgaggccgat agcttcctgg tcgatttcag ctaccagcca
    18301 ccctatccct cgccttatca gtttcagctc gagccttatg acaccgccaa tgacggcgac
    18361 gacacgatca gcggtggcgc cggcaacgac accatcgtgg gcggcaacgg caacaacaag
    18421 ctggacggcg gcgacggcgc cgacacgctc accgcgggca gcggctcgga ctacctatat
    18481 ggcggcccgc gcggcgacgg ttttctcgac atcctgaccg gcggcggcgg cagcgacacc
    18541 ttcatcctca gctacggcag cgacgccacc ggctcaagcg gcgacttctg gtcgggcttc
    18601 ttcgacgcgc agacctcggc catcggcggc aacgtcgtca gcaacctgct ggccagcgcg
    18661 ttcgcgaaag gcgtgggcga catcgccggc gcgatcgccg ggccgatcgg cggcgccgtc
    18721 ctctccgact tcgtcgattt cctcttcggg ctcgattcga cgccaccgcc gaagccgcag
    18781 accacggacg tcatgatggt caccgacttc gacccgagca tggacgtcct gatcctgccg
    18841 ctgatcacca acgaccagga cggcaaggac ctgaccatcg aggccgactt cttcgccgag
    18901 ttcgcggacg gcagcaccaa tgcctggggc ctgaagttca gcgaggacag cacggagttc
    18961 gccgagatcc gcctcgcgga cgactatgtc gccgcactcg gctatgcgcc taccgacagc
    19021 gaggcgccgc tccaggacat cctggacgcg ctcaaggtct ccgcagcgct catcgagccc
    19081 gacggaacgt tctcgacgct ggcgagcgcc gaagttctgg ccctgctgcc gtcgggcggc
    19141 ttcacgccac cggcgaatgc cggcatcccg agcggtgcca ccgtcgacgt cttcggcgcg
    19201 atcggcggcc agctcaccgc gaccggcgga gtctacacga cgattgccgg cacgcagtac
    19261 tcggacgcac tgacggtcaa cgcggccatc gtcgatccgg cgacgatcag ggtgagcgat
    19321 ctggccagcc agaattccac cgtgttcggc ctcggcggcg gcgacgtgct ctatggcggg
    19381 ggtgcttccg acacgctcgt cggcggggac ggcgacgaca cgctctacag ctttctcgac
    19441 gcgctgggtt ccggcgagag cctgtctggc ggtgacggcg acgacatgct ctacggcggt
    19501 gcttcgggcg gggtgttcga tggtggcgcc ggcaccgaca ccttcggtgt cgtctacggc
    19561 aacctcctaa agaacgctct cgaccagggt cccgacggca tgcagctcgt ggtcgatctg
    19621 acccagggct acgccgccga gcgaccggcg agcgacgatc ccgatccggg caccgccccg
    19681 gtcggcgcca ccccgccctt cgacgacagc gtgccgaacg cctacaagct cgtggacatc
    19741 gagaacgtca tcggcggccc gctcaacgac tggatcaggg cggcccaggg ctcggtgatc
    19801 gagggcggcg ccgggccgga ctatctcgac ttcggtgcgg gcaatgtcgt tctgtcctat
    19861 gcgtcgtcga acgcaggggt gaacgtcaac accagcgtgc agagcccgta cgctcaaggc
    19921 ggcgacgccg cgggcgacta ctatgccaat ctcggcagca cgacccagtc gtcgctgtcg
    19981 gcgttgatcg gctcggcgaa cgccgattcc ctctacgtcc tggcatccgt cgtgaacgag
    20041 gccgaggctc tctggatcaa tgtcaccggc ctcggcgggg ccgatgtgtt cggcgccttc
    20101 agcaacgcca atcccgacaa cagcgaggtc accatcaccg atttctcgct ggccgatggc
    20161 gacaggatcg atctgcggcc gcagggcgtc acctcgttct ccgagggcgt caccattgct
    20221 tatgcggctg ataccgtcat cgatatcaac gaggcttcct cgtcgggact ccagatcgtc
    20281 ctgcagggct tcgacagcac gctatccgca ggcgatttca tcttctcgca ggcggtcagc
    20341 ggcacggcca tcggcaatca cacggacgaa ggcttcgccg gcggcccggg tgacgacgtg
    20401 atcgaggggc ggggcgggaa tgacatcctc tatggcaatg gcggcgacga caccctgaag
    20461 gggcaggccg ggcaggactg gttgtatggc ggcgacggcc acgaccggct tttcggtggg
    20521 gcgggcaacg atgcgatcga cggcggcagc ggcgacgacc tcgtccgtgg cggcgccggg
    20581 gacgaccaca tcgccggcgg cgctggcgac gacacgatcc gcggcgacgc cggggcggac
    20641 acgatcgacg ccggggaggg cgacgatttc gtgctcggcg ggctgggcga cgacatcctc
    20701 gtgggcgggc tcggcgacga cgtgatcctg ggcggcgccg gcgtggacac ggccgtcttc
    20761 acgggcgctt tttccgacca cctggtcacc ggtttcggca gcctcgcccg gaccgtcgag
    20821 gacctggccg gcggcggtag ccgcgatcgc ctcgccgatg tcgagctgct gcagttcgac
    20881 gacggcatcc tcgatctgcg cagcggcgcg ttcgacgcgg gcggcgtctc ctcacccgcc
    20941 gtcgaggccc cggtcgccgg ccccggcttc gctgccgatc tcgagccact gctgacgccg
    21001 gtggtgtagc gggcgcgcca tgaccaacgg gcggtcgccc tcgaaggcgc gccggcgatt
    21061 ccaggggggc gggagatgat caggcaagcc atcgatgcag atgaatcggc catccgcgcc
    21121 tgcgccgagt gcgcctacgc ccgctatgtc gccgcgatcg gccgcaagcc ggcccccatg
    21181 gtcgccgatt tcgccgcgca gatcagggcc ggccaggtcc atgtggcggt ggacgacgcg
    21241 gggcttgcgc agggattcat cgtgttcttc ccgaaggacg ggcagatgtt cctcgagaac
    21301 gtcgctgtgc ggccggaagc ggcagggcag gggacaggcg gggcgctgat ccggttctgc
    21361 gaggacgagg cgcggcgcgc gggcctcgca gccgtgcgcc tctacacgaa cgcgaggatg
    21421 acggagaacc tctcgctcta tccgcacctt ggctatgtcg aggtggagcg gcgggaggag
    21481 gacggcttcg atcgggtgtt cttcgagaag gggctcgggt gagccgagga tctcagtgcc
    21541 cgctcggggc cttcgcgagc acggacagcg gaatgaagaa gcggttgtcg gtgtcggcga
    21601 tgtggcgcat gccgtggtgc tggtagaagc gggcggcgtc ttcgtcgagc gcgtcgacga
    21661 tcaggaagtg agcgccgatg tcggagcgga tggcgcggct cgcggcgtcc gtcaggagcg
    21721 cgccgccgag cccctggccc tggaagcgcc ggtcgacggc gaggcggccg acgaggacgg
    21781 cgggcagggt cgggtagcgc ggcaggctgc ggcgccagtc ctcgtcgaga tcggtgaagg
    21841 gcacctgtgc tgccgagagc gtgtagaagc ccgcaaccgt ggcggttggc ttgtgtagca
    21901 cgatgaacgc cgtggcgatc cgccggcgga catcctggcc gatccgggtg cggaagtagt
    21961 gatcgaggct gtccctgccg caggtgaagg acgtgcggtc ttccttctcg aggggcttta
    22021 cggcgaaggg ccgcgaggtc attccgtggc gatcaggcgc cgatggctgg cgatggcccg
    22081 ctccatggcc ggggcgatcc cgggcgggtc gagcagcgcc gcggcgagtg cttgctgccc
    22141 ctgccggctg agccggatca cctcgacctg ctcgatcgtc tgccgtgccg cggcgctggc
    22201 ggcggcgacg acgaaatccg tcatcgagcg cccctcgatc tcggcggcgc gcttgagcag
    22261 ggcgtgcacc tcgggcgtga cccgcgcttc gaggcgtgcg tccttctgca tggtgggctc
    22321 cgcggttctc ctcattcgag catgatacgg ctctctgccg tacaattcaa gatggcatcc
    22381 acccctccct gcggcgcttg gacaggtccg ggtcccagtc gggggcctcg aggttgcagg
    22441 ggtagccgag ggcgtggtcg cggggcgggt cgatgacgta tttgcggtcg aagggggtcc
    22501 agtcgtaggg ctcgccggag cggatgatgt ggggccagtc ggggtgggcg aagatcgggc
    22561 ggccgatggc gcagaggtcg ccggcgccgc tggtcagcac ctcctcggcg tcgctgccct
    22621 cggcgatgcc gccattgacg atcacgggct tgccggaggc ttccctgagc gctcggggca
    22681 tgggcgtggg gtcgctcggg tcgcggttgt cggaccaggc gaaggacgac cagtgcagcg
    22741 cgtcggcggc gcagtcggcg agggcggcac ccacttcccg ggcataggcg acgccgccct
    22801 gccaggcgcc ggtgaaatcg tcgacgccgt cctggctgag gcggagcgtg atcagcttgt
    22861 cggggccgat ggcggcgcgg gtccgctcgc aggcgagctt cgcgaggcgc acgttgttct
    22921 cggccgagcc gccccaggcg tcggtgcgcc ggttcagctt cgggtcgatg aactggtaga
    22981 gcaggtagcc gttggcacca tggagctcga cgccgtcgaa gccggcctcg acgccgcgtg
    23041 ccgccccttc ggcgaagccg tcggcgatcc gctccatctc ggcttcggtc agggcgcggg
    23101 ccggggggaa gccgaccttc ggccaggcgc cctcgatcag ccggtcgtgc ttctcctggt
    23161 cggtgtcggt gtagagcacc cagcccgggc tccgtgcggc gctggcgctg acgggttgct
    23221 cgccctcgtg caggcagcgc gggtcggaga cccggccgcc atgcatgagc tgcaggacga
    23281 tggggatgcc gtgcgcgtgc acggcgtcgg tcacgcgccg ccagccctcg atgtggcgct
    23341 tcgtcaggca gccgggctgg cccagatagg cgacgcagcc gagctcgtcg ttctgccagg
    23401 tgccctcggt gatgatcatg gaaacgccgc ctctcgcccg gcgggcgtag taggcggcca
    23461 tctcggccgt gggcgtgccg tcaggctcgg cgcattgccg ggtcagcggc gccatgacga
    23521 cgcggttctt caagcgctgc gtgccgaggg tgaaggcgga gaatgtcgtt tcgagcatgc
    23581 ggccgtttcc ttgtcttgtt gcttggctgt ggttcaggcc caggcgacct cgccccgcgt
    23641 ggcgtagccg ccgtcggcgc gccgcaccca g
//
LOCUS       DAOTFI010000038        23584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2528_length_23584_cov_6.724510, whole genome shotgun sequence.
ACCESSION   DAOTFI010000038 DAOTFI010000000
VERSION     DAOTFI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 23584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23584
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_23584_cov_6.724510"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="P5558_05595"
     CDS             complement(<1..440)
                     /locus_tag="P5558_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY23814.1"
                     /translation="MKHQAGLATVLAVGLALVAGPAPANAQDADKPNILVIWGDDIGQ
                     SNISAYTRGLMGYETPNIDRIANEGMLFTDYYGEQSCTAGRSSFIMGQSVFRTGLSKV
                     GLPGADIGMREEDPTIAGLLKAQGYATGQFGKNHLGDKDEMLPT"
     gene            591..1595
                     /locus_tag="P5558_05600"
     CDS             591..1595
                     /locus_tag="P5558_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HRY23815.1"
                     /translation="MPSRFITTAVRGFGPLPRIVREAVGERAANLLFTDAGVPLALLD
                     RPDARLPLVDMIAIFERAALLLDDPLLGLDVGRAMSPETYGDWVRYALAGRTLRHGIA
                     RLCRAMRYYQAAGNIRAEVHGNLVRLAYHQSVVDAARARQHADHALLPLLAFVRRFLG
                     PAWQPHWVELPYRPAAGTDELATRLGVEVRFGRPGVGMVFERDFLDAPGNTAPPAFAA
                     LRRMVEGRPDGEVTAALIDVVDLRLTDGEVALDGAASRLGLQRRALQRHLQAEGRSYR
                     QIVDAARRAQAEKALATTARPIAEIAQSLGYGEPAHFSRAFRRWHGCSPNSWRRERAG
                     "
     gene            complement(1610..2437)
                     /locus_tag="P5558_05605"
     CDS             complement(1610..2437)
                     /locus_tag="P5558_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY23816.1"
                     /translation="MPATGKAERTGWARSGARGEDEAGGLLGLLAGRAPDRRKGPLYR
                     QIADIIREPMLAGRLRPGSILPREADLAKLFGVSLITVRHALRELEAEGRVRKQSAKP
                     SIVTSPPPKPGASFSFDTLASIIASTRGREIEILDYRRRRSARAQEVFGLGPGDHTFC
                     LQAILLQDSRPIAFNTFHFPPAVGDHLRREHFDDVVVFRSVQRHLGIRLRGAKVTVWA
                     EVADTELARRLDCDEGAPIMAMEMIYLDEHGAPVELTINRNRADEFSLTFEAESEPG"
     gene            complement(2421..3200)
                     /locus_tag="P5558_05610"
     CDS             complement(2421..3200)
                     /locus_tag="P5558_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY23817.1"
                     /translation="MNVDLEGKIALVTGAATGIGKASAESLLANGATVIFTDRDAARL
                     RETVAGRGDAVVIDVADRSSIADGLAAIMGRHGRIDILVNNAGIGVNAADRKTIDEFP
                     IPVWDQILAIDLTGVFLVSRVVAAAMKARGSGAIVNIASTTGLAPLRLQSPYIAAKAA
                     VINLTRSMAIELAGDGVRVNAVAPGSTATEAWERWMQDPASRAQDLHAGQMAAIPMKR
                     PATTAEIANGVLFLASPAASYVTGHTLVIDGGWTAGYARDW"
     gene            complement(3224..4498)
                     /locus_tag="P5558_05615"
     CDS             complement(3224..4498)
                     /locus_tag="P5558_05615"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY23818.1"
                     /translation="MILLFLALAILIIGGLPIAFAFGLSAMAGLYVEDLAFLNVASRM
                     FAGLDSFVLLAAPFYILVGEIMVRARLADRLIMLSTIIVGRVRGGTAYANVVASILFA
                     GISGTAIADIASLGRVFINAMPKEGYSRAFAAALTVASAIIGPIIPPSVIMVIYAAVA
                     QVSVLTLFLAAIVPGLLLGLSCAVVVFVKSLRGELPQSAMTVPAGQRLPALRDGLVVM
                     TLPAFILFGTVSGVFTPTEAGGIAVVLAILLGTVVFRELAWRELLMSFRAAARLTAAL
                     LLVLATVLIVNYVLILGGVQGITRDLLAHLSDYPFLFLLVCAAFFVVVGLVLDAGPAL
                     LLLAPLLLPITRQMGIDDNQFSMVMLIAVTMGLITPPVGVCLFVTCRIGNLTMGAIWQ
                     ELRWFFLAEIAVLVLLCLFPILSTGLPTLVGR"
     gene            complement(4495..5016)
                     /locus_tag="P5558_05620"
     CDS             complement(4495..5016)
                     /locus_tag="P5558_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY23819.1"
                     /translation="MTYRALRTTVRVLVDGADLLAGLVLIAVTILNLAAVFMRYVMLD
                     SISWSEEMMRYGAVWVTFLAAAGVSLREEHLSLELFRHVGGPACRKLHSALIHLLAAC
                     FAGVVLYQGVIYCLKNGMQTSPTLGLPMVYAYGALVIGGGLLLLAELAKAVDVFLPLD
                     PTDAADPTGAAGA"
     gene            complement(5086..6090)
                     /locus_tag="P5558_05625"
     CDS             complement(5086..6090)
                     /locus_tag="P5558_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY23820.1"
                     /translation="MATLAIGVVLGSATAAEVKLRFSVGANESPTDGMSVGLRAMRDY
                     VAYKSGGEMEIEIFWNTLGGSLQVTEQVKNGTLDMALTDDSVLGSFFPPMQAFQIPYL
                     FGSSAAAWEFANTEIVEDLTKEMLAATGIRTLGFSQNGFRNLTNNEREIKTPDDLAGL
                     KMRTMQSQVYVEFMGAMGASATPIAWPELIPALRTGVVDGQENAAITVLDAGIYEVQK
                     YMSVNEHIYGFHLIIINNDVYEGLTADQQKILHEAVALHSAAANAIKTMADITAVDAL
                     RDKGMTIHVNSPSEKQLFREQAQQAVIDFIAGQIGQETVDRVLAAAAEAEARLYGQAE
                     "
     gene            6294..8702
                     /locus_tag="P5558_05630"
     CDS             6294..8702
                     /locus_tag="P5558_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY23821.1"
                     /translation="MSMSATKRPRPQRQGANLPDTGLLLELFEQMVLLRRFELAARAA
                     CRGGETPGFLHLYIGEEATAVGVCAHLTPRDWVTSTHRGHGHALAKGSNPGAVMAELF
                     GKAGGICGGRGGTMHLYDRTVGLFGTNGVVGAGIGHAAGIGMGARLLGQDAIGVAFFG
                     DGASNHGAFHEGLNFAAVQRAPAVFVCENNLYATATPLASITLNPEIARRADAYGMPG
                     VAVDGNDVFAVWLAMQEATERAHSGGGPTLIEAKTYRTVGHHEGDPVLGTYRTQEELD
                     AWLQRDPIDMFRRRLIADFGVADEATLAAIEAKIDRVVEEALAFARESPEPDPRTARD
                     HVFAEPLNPAVALRRRPAGATRVQGWLEAVRDGIAEEMRANPTIQYLGEGTGERGGSF
                     AHTKDLWQEFGPARMVDTPISEQGFTAAAIGASAIGARSVSDLMFADFAFETAGQIFL
                     QAARLRYMSNGAINAPVVVRASAGALRSSGPHHSGSYHAVFAHMPGLIVCLPSTPADA
                     KGLMKTALRAADPVIMLEPKALFASKGPVPEGEHFVPFGVARIARQGGDITVVATGQL
                     VHTAIEAAERLSVAGIEVEIVDPRTIMPLDVETIVLSVRKTHRLLVVDEDWAMCGLGG
                     EIAQTINELAFDELDAPPGRLHTAPTSHPFAPALERAMLVSADRIVAAVRDVIEGRPP
                     VPDHWRSTTGLAAPVAAPPATTRAPEPRPAARPKAASGEDEPILMPFGDLTVSEGTLV
                     GWRKAEGDRVAEGELVAEIETDKSVMEIEAPCAGTLSRIEKPVGAVVAMGVRIGSIRR
                     ANRS"
     gene            8947..10197
                     /locus_tag="P5558_05635"
     CDS             8947..10197
                     /locus_tag="P5558_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23822.1"
                     /translation="MVHSIVHPSRRRLLAGIGGATLLGGIGAPHIVRAQADTVRFGHL
                     TPLTGFLGPLGAYAQMGVTLAVEEINAAGGVMGRPIELLLEDSVNPATASTKAERYIT
                     RDNVVCIIGEISSASGLAIAQVVERHQKLFLNTGCNSDALRGSDCNRYMFHVEACNTQ
                     YVKAVGTALLQRGLIDGKKIYFLTADYAYGHDLSRVGKRFVEGNGGEVAWEDLVPTDS
                     TDFSAYILKIRQAQPDLIVSNLAGNQITNFVKQYAEYGLDIPYAGFGFDTALAWGAGK
                     GTFGGEWPVVWHHDLDVPASLAFVDAFTTKFGKPPENQAWGDYVAAKVLAAAMNEVES
                     TDTAALLEHFGKEPKLDILKGREGYFRDWDHQLMQEMYTMTARPVDEVQNEWDLMVLG
                     PPVPPADEALESIAPTPEENACEL"
     gene            10279..11160
                     /locus_tag="P5558_05640"
     CDS             10279..11160
                     /locus_tag="P5558_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY23823.1"
                     /translation="MPLELVFILEQVLNGLLVGVYYLLIALGLSLIFSLGGIVNLAHG
                     GFYAIGAYLIVILTGEIGFFGAFVVSPILVAFLGMATERVFFRRFYRLDPMLSLLLTF
                     GLAMIIEQGLRMIFGAAPIPYAIPGFLHGQVLLGKLIFSSYRLAILIVAILAVAGLWF
                     LLQRTPFGRVVRAGVQNPDMVGALGISLSPYFTGVAALAMGLAGLAGVLMAPITGVHP
                     AMGLEILIPAFVVVVIGGLGSFWGVVAAAALVGIIRGLTVYYYPPASEASMYLLMLAV
                     LLLRPRGMFGERIARFE"
     gene            11157..13682
                     /locus_tag="P5558_05645"
     CDS             11157..13682
                     /locus_tag="P5558_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HRY23824.1"
                     /translation="MMLARHVNLLVLALALVALPFALFALGLTYGSATDVVVLGLAAM
                     ALNLLVGYTGLVSFGHGAWLGLGAYAAALLQLHFFPGQMLLPAVLGVIIVGLIALLLG
                     PLILRRRGVYFSLITLALAALAFAIAYRWTALTGGESGLGGIVRSDFLGIDLDRSDAA
                     YYAFVAIVAFLVATMLQRLVRSPIGTVLVAIRENEERTRFLGYSTTAYKVVCFTVSAM
                     VTGLAGVLFVFQHRFASADPLAVFFSGDLLAMVIIGGMRSFLGPALGALFYVLFREFL
                     SMWTPDWLLYFGFLFVAFIMFSPTGLMGVAGRLLAPFRHQPVSAAAMAGRATALERPL
                     PKALAGHDSGSGPILVVERIAKSFGAIQAMRGASFSVARRTLHALIGPNGAGKTTAFN
                     VVSGMFPPDRGKVLLDGQPIEGLDPNVVCRRGLARSFQITNLFNGLSVEENLRLGVQA
                     THAARFSPWRDAHGIASINQETAEIMAFLGVEGMEQAVAGTLSYGGQRLVDMGLALTS
                     KPRLLLLDEPLAGLAATERERIGRLIKRISADIPVLLVEHDIDRVFELADAVTVMNNG
                     EVLIGGTVEEVRSDPRVQEVYIGSGTAALAGREMVSVAEPEVLLGARGINTYYGKSHV
                     LTDVDLDVHKGEIVALLGRNGAGKSTFLKTLIGIAPPASGTLVMQGEDLAGLPSAEIA
                     RRGIGYVPQERALFQGMSVRHNLELGRLKRRTGAGTHWADDDILAFFPRLAVRLDTAA
                     DRLSGGEQQMAAVARALVGDTKLLLLDEPFEGLSPAVTEELFDVFDRLRREVAILIVD
                     HNLDLVLNLADRAYVLERGQVIHEGPSRPLAHDYDLRREILWL"
     gene            complement(13830..14822)
                     /locus_tag="P5558_05650"
     CDS             complement(13830..14822)
                     /locus_tag="P5558_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY23825.1"
                     /translation="MTKHGRLVAAGSVVGLALMVAAPALAQEVNIYTSNPVQSIEAIT
                     EVARTAMPDVQLNAITGGSGVLLRRIESEADKVQADIFWSSSANTLGAFESLFESYDS
                     PALAAIPEGLHYPGNRFLPTNVHVSVIMVNEDLLGDLPMPETWSDLADPVYQGKITVP
                     DPANSSTGYTILLGARELLDEAAFQALAGNVVVTGSSSAVPRGVAMGEYPIGLTFEAT
                     AYAYIDGGQDELKLVYPSEGTFVTPEYLGLVAGAPAGDVACQAVDMLLSAEAQTALLV
                     NGFRRPSRSDIEVSKYLALPELADVEVFAVDEAAAANEREPFLEMIRSIAGVAQ"
     gene            complement(14896..16488)
                     /locus_tag="P5558_05655"
     CDS             complement(14896..16488)
                     /locus_tag="P5558_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY23826.1"
                     /translation="MFVFIVYPIINIVTSSFSNGGGWSQILSEQRYGEAIANSLMLAA
                     IVTVACTLVGVPLAYVTARFRFPGKSLIVFLPLITLVIPEVIAAQTWLMMLGNNGLIT
                     RFFREYGIILPSFYGWFGLVTSMTFIYYTYVYIGTVAAISGFDAHLEEAAQSLGSSPA
                     RARLKVMVPVIAPAILSGALLVFTMVIGNFAISIILGHQVQLLSVMTYRAAVSEGGVN
                     PVMQSTLASVSILIVMVVLFINRWIVARGRYEVVQGRGAPPSALRGLEGVLIALAAGT
                     VVLISMLPMISVILGAFTRARGPVMQWGTWTLENMERVFVTAPQPLVNSLVYAGLATC
                     IGISFSTVVSYMIVKKRNLITPLLDYISVVPLALSGTVLGIGLIISFHTGWLPLTGTA
                     SIIVLAYVVRRLPFGMRNASSTLYNVPNSIEEASISLGSPPFRTFVRVVLPMMAPAIA
                     AAAVLTWTTSVAELSASILVYSGGRETLPIQIFRLLDSALMAYASACGLTLIGVILLP
                     IMIAIKVFRMDLFGVGKPRSES"
     gene            complement(16548..17618)
                     /locus_tag="P5558_05660"
     CDS             complement(16548..17618)
                     /locus_tag="P5558_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23827.1"
                     /translation="MSISISGLAVSYGQADVIADLDLRIDEGNFFTLLGPSGCGKTTL
                     LRTIAGFVPAARGRILFGDVDVTRLPAHKRDIGMVFQDYALFPDKTVFENVAYGLRAR
                     GERGAVLEGRVMKALERVDLGPFRSRKPAALSGGQRQRVALARALVIEPQVLLMDEPL
                     SNLDARLRLQVRETIMELQREARITTVFVTHDQEESLSMSDRIGVMNKGRIEQIGSPK
                     EIYATPATTFVADFVGAANRIPVTLGQGADATRPTVRFGDVVLRARCAGPVPNGEGVL
                     VLRPEDLGMDDSPTTDGAASSLPGSVILRQYLGARTAYKVRLGDGSVLAVDLHGEDHD
                     RFSAGQHVSVSVNPEKALVLAR"
     gene            complement(17615..18439)
                     /locus_tag="P5558_05665"
     CDS             complement(17615..18439)
                     /locus_tag="P5558_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRY23828.1"
                     /translation="MASTANLVTCLRMAIELAETSREMIGRSLVDGAFRHGDATLKED
                     RSFVTATDAAVEARLREIISRRFPDHGVIGEEHGEHGRGCSHVWVLDPIDGTAAFMAG
                     IPVFGSLIACCVDGDPVIGVMDFPALGARFVGVAGEPTRLNGNVLKTRPCAELAGAIL
                     SASSPDFFPPGDIQAFERLRSRTAWRIYGAAALAYGRLAQGRIDLSIDSGLKIWDIAA
                     FAPIVSGAGGRITDWAGAPLTIDSGPRVLAAGDPAMHAVALELLGREDAAKRRAGT"
     gene            complement(18917..20176)
                     /locus_tag="P5558_05670"
     CDS             complement(18917..20176)
                     /locus_tag="P5558_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY23829.1"
                     /translation="MRAGLTVLAGAAVLTGTAAGLAQEAGFSDIERGRYLARVGDCVA
                     CHTAEGGEPFAGNRPIPTPFGTLYSANITPDRETGIGGWSDAEFERAMTQGMAPGGKR
                     LYPAFPYPFFTKVTRDDIRAIKAWLDTLEPVRQEVDPPELPWPLSWRALLAGWNALFF
                     EEGVFTPVPGKSAEWNRGAYLVEGLGHCAACHSPKNLAGAVELDEAFEGGFGEGWFAP
                     DLTGDLREGLGEWSVEDIVEYLKTGSNRFSSAGGPMAEVVERSTQHLTREDLRAMAVY
                     LEDLPAAEEVAEPGDVRDLDELLMAEGEALFVDNCTGCHLMSGEGQEGVFPALAGSSV
                     LQQEEPHSAIQIVLGGAEKPSTEAKPTPLAMPALGWKLDDDEVAALLTYLRNAFGNSA
                     SPVEHSTVTDVRSIIEEREGTAALASE"
     gene            complement(20178..21941)
                     /locus_tag="P5558_05675"
     CDS             complement(20178..21941)
                     /locus_tag="P5558_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRY23830.1"
                     /translation="MPTKLPPVDVLLVGFGWTAAILGEELAQAGLEVLALERGQFRDT
                     VPDFAMPQIQDELRYAVRHEMFQEPARQTITFRNNHAQTALPMRRLGSFNPGTGVGGA
                     GVHWNGQHWRFLPSDFRQRSHVEERYGADFIPEGMTIQDWGVTYEELEPHFDRFEYLC
                     GTSGVAGNLNGEITAQGNPFEGPRSRDYPNPPLERSLGTLLFDKAAREAGFHPFPAPA
                     SNTSRPYRNPLGVQMGKCTYCGYCEWFGCANYSKSTPQTTILPVLLNRSNFSLRTNCN
                     VTRIKLDRSGKRATGAIYVDSSGEEHEQPADLVILSAYATHNVHLLLLSGIGEVYDPK
                     TRSGTLGRNYAYQMVSGVDVFVEENLNPFMGAGALGQAIDDFNGDNFDHGPHGFIGGA
                     YVALWTTGGRPILQHQLPQGTPEWGSAWKQAMAENYLRSASLSVHGSVMSYNDAHLDL
                     DPTYRDAYGDPLMRLTFDFHDNERRMSRFVTARAEEIARAMRPRSYTVNQRQGPYSIV
                     PYQTTHNTGGAVMGDDPSTSVVNRYLQHWDVPNLFVMGACVFPQNAGYNPTGTVGALT
                     YWAAEAIKTRYLKNPGPLVDA"
     gene            complement(21956..22564)
                     /locus_tag="P5558_05680"
     CDS             complement(21956..22564)
                     /locus_tag="P5558_05680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HRY23831.1"
                     /translation="MVPALTRRPGADGHFTDAERAFVDAATARIIPTDDTGAGAREAG
                     VTQFIESQLAGDFGDAARWYMQGPFKDGTDEQGYQLPLTPAQLYSAAIRAVDQHCRDF
                     FGGRVFADLDAEEQDEVLRGLEAGEPELGDVDSRSFFKMLRANTVEGFLSDPMYGGNQ
                     GFVGWRLIGFPGPRYDYTRFIGRHGEPFPLPPVGILGRDGFI"
     gene            23069..>23584
                     /locus_tag="P5558_05685"
     CDS             23069..>23584
                     /locus_tag="P5558_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY23832.1"
                     /translation="MNELDAIVAIGAGAVLLLGVTSGCIRNRLWISEPAVCLAVGVAL
                     SPLQFEFLGVAPGSDEYLTLVQQVARLTLAISVMGAALRLPQDYVRRHWRELTLILGL
                     GLPMMCLSGAFLAYLMLSVPLLTALLIGSVLAPTDPVLSDSIVAGRIADGHIPARMRN
                     SLTAESGANDGL"
BASE COUNT         3712 a         7927 c         8078 g         3867 t
ORIGIN      
        1 ttggtcggca gcatctcgtc cttgtcgccg agatggttct tgccgaactg gccggtggcg
       61 tagccctggg ccttgagcag gccggcgatg gtcgggtcct cctcgcgcat gccgatatcg
      121 gcgcccggca gcccgacctt cgagagcccg gtgcggaaca cgctctggcc catgatgaac
      181 gaggagcggc cggctgtgca ggactgctcg ccgtagtagt ccgtgaagag catgccctca
      241 ttggcgatgc gatcgatgtt cggcgtctcg tagcccatca ggccccgcgt ataggcggag
      301 atgttcgact gaccgatgtc gtcgccccag atgacgagga tgttcggctt gtcggcgtcc
      361 tgcgcgttgg ccggagccgg cccggcaacc aacgccaggc ccacggcgag cacagtcgcc
      421 aagcctgctt gatgtttcat gcagcgtctc ctcccgggag ttgttctagc gtcggcccaa
      481 aatgcctcgg catgcgtgtc cttggcacgg acggacatag cgctagttct tggacggcgc
      541 ttaccatttt gcgtctgcga ttcattggag cacgaggagc tcggcgagcc atgccaagcc
      601 ggttcatcac caccgccgtg cgtggcttcg gcccgctgcc gaggatcgtg agggaggcgg
      661 ttggcgagcg cgcggccaac ctgctcttca ccgatgccgg cgtgccgctg gctctgctcg
      721 accggcccga tgcgcggctg ccgctcgtcg acatgatcgc gatcttcgag cgggcggcgc
      781 tcctcctgga cgatccgttg ctgggcctcg acgtgggccg cgccatgtcg ccggagacct
      841 acggcgactg ggtgcgctac gcgctcgccg ggcggacgtt gcgccatggc attgcccggc
      901 tctgccgggc catgcgctat tatcaggcag cgggcaacat ccgcgcggag gtccacggca
      961 atctcgttcg actcgcctat caccagtctg tcgtcgacgc ggcccgtgcg cgccagcatg
     1021 cggaccacgc cctgctaccg ctgttggcgt tcgtgcggcg gttcctcggg ccggcctggc
     1081 aaccacactg ggtcgagctt ccctaccggc cggccgccgg aaccgacgag ctggccaccc
     1141 ggctcggtgt cgaggtgcgc ttcgggcgac ccggcgtggg catggtcttc gagcgcgact
     1201 tcctcgatgc gccgggcaac accgcgccgc cggcgttcgc cgcgttgcgt cggatggtcg
     1261 aaggccgccc cgacggcgag gtcaccgcgg ccttgatcga tgtcgtcgac ctgcgcctca
     1321 ccgatggcga ggtggcactc gacggggcag cgagcaggct cggcctgcag cggcgggccc
     1381 tgcagcggca cctgcaggcc gagggccgat cctaccgcca gatcgtcgat gccgcccgcc
     1441 gcgcccaggc cgagaaggcg ctcgcgacca ccgcccggcc gatagcggag atcgcccaga
     1501 gcctgggcta tggcgagccg gcccatttca gccgtgcctt tcgccgctgg cacggctgca
     1561 gcccgaacag ctggcggcgc gagcgggcgg gctgagcggc gagtcgcctt cagccgggct
     1621 cgctctccgc ctcgaaggtc aggctgaact cgtccgcccg attgcggttg atggtcagct
     1681 cgaccggcgc gccgtgctcg tcgaggtaga tcatctccat ggccatgatc ggcgcaccct
     1741 cgtcgcagtc gagccggcgg gcgagctccg tgtcggccac ctcggcccag acggtgacct
     1801 tcgccccccg gagccggatg cccagatggc gctgaacgga gcggaagacg accacgtcgt
     1861 cgaagtgctc gcgcctcaga tggtcgccga ccgccggcgg gaaatgaaag gtgttgaagg
     1921 cgatggggcg ggaatcctgg agcaggatcg cctgcaggca gaaggtgtgg tcgcccggcc
     1981 cgaggccaaa gacttcctgc gccctggccg agcggcgacg gcggtagtcg aggatttcga
     2041 tctcccggcc cctggtcgag gcgatgatcg aggccagggt gtcgaagctg aagctcgcgc
     2101 cgggcttcgg cggcgggctg gtgacgatgc tgggcttggc cgactgcttg cgcacccgcc
     2161 cttccgcctc gagctcgcgg agcgcgtgcc gcaccgtgat gaggctcacg ccgaagagct
     2221 tcgcgagatc ggcctcgcgc ggcaggatgc ttcccggccg caggcggccg gcgagcatcg
     2281 gctcgcggat gatgtcggcg atctgccggt agagcggacc cttgcggcgg tccggcgcac
     2341 gtccggcgag gaggccgagc agcccgcccg cctcgtcctc tccccgtgcc ccggatcgag
     2401 cccagccggt gcgctctgcc ttaccagtcg cgggcatagc cggcggtcca gccaccatcg
     2461 atcaccaggg tgtggcccgt cacgtaggag gcggccggcg acgccaggaa gaggacgcca
     2521 ttggcgatct cggcggtggt ggccggccgc ttcatcggaa tcgccgccat ctgcccggcg
     2581 tgcaggtcct gagcccggct cgccgggtcc tgcatccagc gttcccaggc ctcggtcgcg
     2641 gtcgagccag gcgccacggc gttgacgcgc actccatcgc cggcgagctc gatggccatc
     2701 gagcgcgtca ggttgatgac cgcggccttg gcggcgatgt aggggctctg caggcgcagc
     2761 ggcgccaggc cggtggtgga cgcgatattg acgatggcac cgctgccccg ggccttcatt
     2821 gcggccgcca ccacccggct gaccaggaag acgccggtca ggtcgatggc gaggatctgg
     2881 tcccagaccg gaatcgggaa ctcgtcgatg gtcttgcggt cggcggcgtt cacgccgatg
     2941 ccggcgttgt tcacgaggat gtcgatccgc ccgtggcggc ccatgatcgc ggcgagaccg
     3001 tcggcgatcg acgaacggtc ggcgacatcg atgacgacgg cgtcgccccg gccggccacg
     3061 gtctcgcgca gccgcgccgc gtcgcggtcc gtgaagatca cggtggcgcc gttggccagg
     3121 agggactcgg ccgaggcctt gccgatgccg gtcgcggcac cggtgaccag agcgatcttg
     3181 ccttcgaggt ccacgttcac ttcatccctc cgggttcatg gggtcagcgc ccgacgagcg
     3241 tgggcaggcc ggtcgacagg atcggaaaca ggcacaggag gacgagcacg gcgatctcgg
     3301 ccaggaagaa ccagcgcagc tcctgccaga tggcgcccat ggtgaggttg ccgatccggc
     3361 aggtgacgaa gaggcagacg ccgaccggcg gcgtgatcag ccccatggtg acggcgatca
     3421 gcatgaccat cgagaactgg ttgtcgtcga tgcccatctg ccgggtgatc ggcagcagca
     3481 gcggcgcgag cagcaagagg gccggcccgg cgtcgaggac caggccgacg acgacgaaga
     3541 aggcggcgca gaccagcagg aagaggaacg gatagtcgct gagatgggcg agaagatcgc
     3601 gggtgatgcc ctgcacgccg ccgagaatca gcacgtagtt gacgatcagc accgtcgcca
     3661 ggaccagcag cagcgccgca gtcagccgtg ccgcggcacg aaacgacatg agcagctcgc
     3721 gccaggcgag ctcgcggaag acgaccgtgc cgaggaggat ggcgagcacc accgcgatgc
     3781 cgcccgcctc ggtcggggtg aagacgccgg agaccgtgcc gaacagaatg aaggccggca
     3841 gggtcatgac gacgaggccg tcgcgcagcg ccggcagtcg ctgcccggcc ggcaccgtca
     3901 tggcgctttg cggcagctcg ccgcgcaacg acttgacgaa gaccacgacg gcgcaggaga
     3961 ggccgagcag cagccccggg acgatggccg cgaggaagag ggtgaggacg gagacctggg
     4021 ccactgccgc gtagatgacc atgatgacgg agggtgggat gatcgggccg atgatggccg
     4081 atgccacggt cagcgctgcg gcgaaggcgc ggctgtagcc ctccttgggc atggcgttga
     4141 tgaagacgcg gccgagcgag gcgatgtccg cgatggcggt gccggagatg cccgcgaaca
     4201 gaatggacgc gacgacgttg gcataggcgg tgccgccgcg cacccgcccg acgatgatgg
     4261 tggagagcat gatcaggcgg tcggcgagac gggcacggac catgatctcg ccgacgagga
     4321 tgtagaaggg tgccgccagg agcacgaacg agtcgagccc ggcgaacatc cggctggcga
     4381 cgttcaggaa ggcgaggtcc tcgacataga gcccggccat ggccgagagg ccgaaggcga
     4441 aggcgatcgg caggccgccg atgatgagga tcgcgagggc caggaagagc aggatcatgc
     4501 gcccgcagcc ccggtcgggt cggcggcgtc ggtcgggtcg agcggcagga agacgtcgac
     4561 ggccttcgcc agctcggcga gcagcaggag cccgccgccg atcacgagcg cgccataggc
     4621 gtagaccatc ggcaggccga gcgtgggcga ggtctgcatg ccgttcttga ggcagtagat
     4681 cacgccctga tagagcacga cgccggcgaa gcaggcggca agcaggtgga tcagggcgct
     4741 gtgcagcttg cggcaagccg ggccgccgac atgccggaag agctcgaggc tgagatgctc
     4801 ctcccgcaag ctcacgcccg cggccgcgag gaaggtcacc cagacggcgc catagcgcat
     4861 catctcctcg ctccaggaaa tcgagtcgag catgacgtag cgcatgaaca cggcggcgag
     4921 gttgaggatg gtgacggcga tgagcacgag gccggcgagc aggtcggcgc cgtccaccag
     4981 cacccgcacc gtcgtccgca gggcccggta cgtcatctat gacctcgtcg cggaggaagt
     5041 cggcgacggc gccgggagcg ccgccgtcgc ccgtccatca gggtgtcact cggcctggcc
     5101 gtaaaggcgc gcctcggcct cggcggcggc ggcgagcacc cggtcgacgg tttcctggcc
     5161 gatctggccg gcgatgaagt cgatcaccgc ctgctgcgcc tgctcgcgga agagctgctt
     5221 ctcgctcggc gagttgacat ggatggtcat gcccttgtcg cgcagggcgt cgaccgcggt
     5281 gatgtcggcc atcgtcttga tcgcgttggc agcagcgctg tgcagcgcca cggcctcgtg
     5341 gaggatcttc tgctggtccg ccgtgaggcc ctcgtagacg tcgttgttga tgatgatgag
     5401 gtggaagccg tagatgtgct cgttcacgct catgtatttc tggacttcgt agatgccggc
     5461 gtcgagcacg gtgatcgccg cgttctcctg gccgtcgacg acgccggtgc gcagcgccgg
     5521 gatcagctcg ggccaggcga tcggcgtggc cgaggcgccc atggcgccca tgaactcgac
     5581 atagacctgg ctctgcatgg tgcgcatctt gaggccggcc aggtcgtccg gcgtcttgat
     5641 ctcgcgctcg ttgttggtga ggttgcgaaa cccgttctgc gagaagccga gcgtgcggat
     5701 gccggtcgcc gcaagcatct ccttggtcag gtcctcgacg atctcggtgt tggcgaactc
     5761 ccaggccgcc gccgacgagc cgaagagata ggggatctgg aacgcctgca tcggcgggaa
     5821 gaaggatccc agcaccgaat cgtcggtcag ggccatgtcg agcgtgccgt tcttgacctg
     5881 ctcggtcacc tgcagcgacc cgcccagcgt gttccagaag atctcgatct ccatctcgcc
     5941 gccggacttg taggcgacgt agtcgcgcat cgcccgcaga ccgaccgaca tgccgtcggt
     6001 cggcgattcg ttggcgccga cggagaagcg gagcttgact tccgccgccg tcgcgctgcc
     6061 gagaaccacg ccgatagcga gggtcgccac cgcgcaagcc gacagcacgc ggcgcgtcgc
     6121 gctcgcaaga ccatgaccac gcatccttgc ctccactgat ctgttgctga tcgcgggcta
     6181 tcctgcccgc ctcgcctgcc gaccggcggg cttgtgggac tcgccggtaa cgattatggt
     6241 attataaact tataagactg gcaagcaagc ggcgccggac agggagtggt ggcatgtcga
     6301 tgtctgcaac gaagcgaccg aggccgcagc ggcaaggtgc caacctgccg gacaccgggc
     6361 tcctgctcga gctgttcgag cagatggtgc tgctgcgccg cttcgagctg gccgcccggg
     6421 ccgcctgccg cggcggcgag acacccggct tcctccatct ctacatcggc gaggaggcga
     6481 ccgcggtcgg cgtctgcgcg catctgacgc cgcgggactg ggtgacctcg acccatcgcg
     6541 ggcatggcca tgccctggcc aagggctcca atcccggtgc ggtcatggcc gagctcttcg
     6601 gcaaggccgg cggcatctgt ggcggccgcg gcggcacgat gcatctctac gaccggacgg
     6661 tcggtctctt cggcaccaac ggtgtcgtcg gcgccggcat cggccatgcg gcgggcatcg
     6721 gcatgggcgc ccggctgctc ggtcaggatg cgatcggcgt agcgttcttc ggcgacggcg
     6781 cctcgaatca tggcgccttc cacgaggggc tcaacttcgc cgcggtgcag cgggcaccgg
     6841 cggtcttcgt ctgcgagaac aatctctacg ccacggcgac ccccctcgcc tcgatcacgc
     6901 tcaatcccga gatcgcgcgc cgtgccgacg cctacggcat gcccggcgtg gcggtggacg
     6961 gcaacgatgt cttcgccgtc tggctcgcca tgcaggaggc cacagagcgc gcccactcgg
     7021 gcggcgggcc gacgctgatc gaggccaaga cctaccgcac cgtcggccat cacgagggcg
     7081 acccggtgct cggcacctac cggacccagg aggagctcga cgcctggctg cagcgcgatc
     7141 ccatcgacat gttccgccgg cggctgatcg ccgatttcgg cgtcgccgac gaggcgacgc
     7201 tggcggcgat cgaggcgaag atcgaccggg tggtggagga ggccctcgcc ttcgcgcggg
     7261 aatcccccga gccggacccg cgcacggcgc gcgaccatgt cttcgccgag ccgctcaacc
     7321 cggcggtcgc cctgcggcgc cggccggccg gcgccacccg ggtccagggc tggctcgagg
     7381 cggtccgcga cggcattgcg gaggagatgc gcgccaatcc gaccatccag tatctcggcg
     7441 agggcacggg cgagcgcggc ggcagcttcg cccataccaa ggatctgtgg caggagttcg
     7501 ggccggcgcg gatggtcgac acgccgatct ccgagcaggg cttcaccgcc gccgccatcg
     7561 gtgcttcggc catcggtgcc cgctcggtgt ccgacctgat gttcgccgac ttcgccttcg
     7621 agacggccgg gcaaatcttc ctgcaggccg ccaggctccg ctacatgagc aatggcgcca
     7681 tcaatgcgcc cgtggtggtg cgcgccagcg ccggtgccct gcgcagctcg gggccgcacc
     7741 acagcggcag ctatcatgcc gtcttcgccc acatgccggg cctgatcgtc tgcctgccgt
     7801 cgacgccggc cgacgccaag gggctgatga agacggcgct cagggctgcc gatcccgtca
     7861 tcatgctcga gccgaaggcg ctgttcgcct cgaaagggcc cgttccggag ggcgagcatt
     7921 tcgtcccctt cggcgtggcc cggatcgcgc ggcagggtgg cgacatcacc gtcgtcgcca
     7981 ccggccagct cgtgcatacg gcgatcgagg cagccgagcg cttgagcgtc gcgggcatcg
     8041 aggtcgagat cgtcgatccg cgcacgatca tgccgctcga cgtcgagacc atcgtcctga
     8101 gcgtgcgaaa gacgcatcgc ctgctggtcg tcgacgagga ctgggcgatg tgcgggctcg
     8161 gtggcgagat cgcgcagacg atcaacgagc tcgccttcga cgagctggac gcgccgcccg
     8221 gccggctgca taccgccccc acctcgcacc ccttcgcccc ggcgctcgag cgggccatgc
     8281 tggtgagcgc cgaccggatc gtggcggccg tgcgcgacgt gatcgagggt cggccgcccg
     8341 tgccggacca ttggcggagc acgaccgggc tcgcggcgcc cgtggcggct ccgccggcga
     8401 ccacacgggc gcccgagccg aggcccgcag cgaggccgaa ggccgcgagc ggcgaggacg
     8461 agccgatcct gatgccgttc ggggacctga ccgtgagcga gggcacgctc gtcggctggc
     8521 gcaaggccga gggcgacagg gttgccgagg gcgagctggt cgccgagatc gagaccgaca
     8581 agagcgtgat ggagatcgag gcaccctgcg ccggaacgct gagccggatc gaaaagcccg
     8641 tcggcgccgt ggtggcgatg ggcgtccgga tcggcagcat ccgcagggcg aaccgctcct
     8701 aggcgacgac cccggatgct cttggcatgc cggagccgcc gttgcgggac cggaccgggc
     8761 tcgtgtcggc cggcgaggct cggagagagc ggcggatgcg gaaatattgg acgatccccg
     8821 tatcgctgga cacgcgggtt gaacggcgct ctactgattc acacaaccgt tgacgtgaat
     8881 gcggatcaat gacgctgcgg cccctcgact gcgaggagcc gcgatagcgc cctggggaga
     8941 gaaaacatgg tgcattcgat tgtccatccc agtcgtcgtc gactgctcgc cggcatcggt
     9001 ggcgccaccc tgctcggcgg catcggtgcg ccgcatatcg tcagggcaca ggccgacacg
     9061 gtccgtttcg gtcatctcac gccgctcacg ggattcctcg gtccgctcgg cgcgtacgcg
     9121 cagatgggcg tgacactcgc cgtcgaggag atcaacgcgg ccggcggcgt catgggccgc
     9181 ccgatcgagc tcttgctcga ggacagcgtc aacccggcga cggcctccac gaaggccgag
     9241 cgctacatca cccgcgacaa tgtcgtctgc atcatcggcg agatcagctc ggcctcgggc
     9301 ctcgccatcg cccaggtggt cgagcgccac cagaagctgt tcctcaacac cggctgcaac
     9361 tcggatgccc tgcgcggcag cgactgcaac cgctacatgt tccacgtcga ggcctgcaac
     9421 acgcaatacg tcaaggcggt gggcaccgcg ctgctgcagc ggggcctgat cgacggcaag
     9481 aagatctact tcctcacggc agactacgcc tacggccatg atctgtcccg cgtcggcaag
     9541 cggttcgtcg agggcaatgg cggcgaagtg gcctgggagg atctggtgcc gaccgattcc
     9601 accgacttca gcgcctacat cctgaagatc cgccaggccc agccggatct gatcgtctcc
     9661 aacctcgccg gcaatcagat caccaacttc gtcaagcaat atgccgagta cgggctggac
     9721 atcccctatg ccggcttcgg cttcgacacc gccctggcct ggggcgccgg caagggcact
     9781 ttcggcggcg aatggccggt cgtctggcat cacgacctcg acgtgcctgc ctcgcttgcc
     9841 ttcgtcgacg ccttcacgac gaaattcggc aagccgccgg agaaccaggc ctggggcgac
     9901 tacgttgccg ccaaggtcct cgccgccgcc atgaacgagg tggaatcgac cgacacggcg
     9961 gccctcctcg agcatttcgg caaggagccg aagctcgaca tcctgaaggg gcgcgaaggt
    10021 tatttccgcg actgggacca ccagctcatg caggaaatgt acacgatgac ggcgcggccg
    10081 gtcgacgagg tgcagaacga gtgggatctg atggtgctcg gcccgcccgt cccgccggcg
    10141 gacgaggcgc tggagtcgat cgcaccgacc cccgaggaga atgcctgcga gctctaggcg
    10201 caggcgcccg ctccgctggc cgggagcggg cgcgccggcc ggtcgttcat tccccctcct
    10261 ttgacgcgag ggctgccctt gccgcttgag ctcgtcttca tcctcgagca ggtgctgaac
    10321 ggcctcctgg tcggcgtcta ctacctgctg atagcgctcg ggctctcgct catcttcagc
    10381 ctgggcggca tcgtcaacct cgcccatggc ggcttctatg ccatcggcgc ctatctgatc
    10441 gtcatcctga ccggcgagat cggcttcttc ggggccttcg tcgtctcgcc gatcctggta
    10501 gcatttctcg gcatggcgac cgagcgcgtc ttctttcgcc gcttctatcg gctcgatccg
    10561 atgctcagcc tgctcttgac cttcggtctc gccatgatca tcgagcaggg gctgcgcatg
    10621 atcttcgggg cggcgccgat cccgtacgcg atcccgggct tcctgcacgg gcaggtgctg
    10681 ctcggcaagc tgatcttctc gagctatcgc ctcgcgattc tcatcgtcgc gatcctcgcc
    10741 gtggcagggc tctggttcct gctgcagcgc acgccctttg gccgcgtggt ccgggccggc
    10801 gtgcagaatc cggacatggt gggtgctctc ggcatctcgc tctcccccta cttcaccggt
    10861 gtggcggcgc tcgccatggg gctcgccggg ctcgccggcg tgctgatggc gccgatcacg
    10921 ggagtccacc cggcgatggg cctggagatc ctgattccgg ctttcgtcgt cgtcgtgatc
    10981 ggcgggctcg gctcattctg gggcgtggtc gcggcggcgg cactggtcgg catcatccgt
    11041 ggcctcacgg tctattacta tccgccggcc tccgaggcct cgatgtatct cctcatgctc
    11101 gccgtgctcc tgctccgtcc gcgcggcatg ttcggcgagc ggatcgcgcg gttcgaatga
    11161 tgctcgcccg acacgtcaac ctcctcgtcc tggcactggc gctcgtggcc ttgcccttcg
    11221 cgctcttcgc gctcgggctc acctatggct cggcgaccga tgtggtggtg ctcggcctgg
    11281 ctgccatggc gctcaatctg ctcgtcggct acacggggct ggtctcgttc ggacatggtg
    11341 cctggctggg cctcggcgcc tatgcggcag ccctgctgca gctgcatttc ttccccggcc
    11401 agatgctgtt gccggccgtg ctcggcgtca tcatcgtcgg cctgatagcg ctgctgctcg
    11461 gaccgctcat cctgcgtcgc cgaggggtgt atttctccct gatcaccctg gccctcgccg
    11521 ccctcgcctt cgccatcgcc tatcgctgga cggcgctcac cggcggcgag agcgggctcg
    11581 gcggcatcgt ccgctcggac ttcctcggga tcgatctcga ccgcagcgat gccgcctatt
    11641 acgcgttcgt ggcgatcgtc gcctttctcg tcgccaccat gctccagcgc ctggtgcgct
    11701 cacccatcgg cacggtgctc gtcgccatcc gcgagaacga ggagcggacc cgctttctgg
    11761 gctattcgac gaccgcctac aaggtggtct gcttcaccgt ctcggccatg gtcaccggtc
    11821 tcgccggcgt gctcttcgtc ttccagcatc gcttcgcctc ggctgacccc ctggccgtct
    11881 tcttctccgg cgacctgctc gccatggtca tcatcggtgg catgcgcagc ttcctcggcc
    11941 ccgccctcgg cgccttgttc tacgtcctgt tccgtgagtt cctgtcgatg tggacgccgg
    12001 actggctgct ctatttcggc ttcctgttcg tcgccttcat catgttctcg ccgaccgggc
    12061 tgatgggtgt cgccggccgg ctcctcgcac ccttcaggca ccagcccgtc tccgccgccg
    12121 ccatggccgg ccgggcgacg gcgctcgagc ggccgctgcc caaggccttg gccgggcacg
    12181 acagcggctc ggggccgatc ctcgtggtcg agcggatcgc caagagcttc ggggccatcc
    12241 aggccatgcg cggggcgagc ttcagcgtcg cccgtcggac cctgcacgcc ctgatcggac
    12301 cgaacggcgc cggcaagacg acggccttca acgtcgtctc gggcatgttt ccgccggatc
    12361 gcggcaaggt ccttctggat ggccagccga tcgagggcct cgaccccaat gtcgtgtgcc
    12421 gacgtggcct cgcccgctcg ttccagatca ccaacctgtt caacggcctg agcgtcgagg
    12481 agaacctgcg cctcggcgtg caggcgacgc atgccgcccg cttcagcccc tggcgcgatg
    12541 cgcatggcat cgcctcgatc aaccaggaaa ccgccgagat catggccttt ctcggtgtcg
    12601 aggggatgga gcaggcggtg gcggggacgc tctcctatgg tggccagcgc ctggtcgaca
    12661 tgggtctcgc cctcacctcg aagccgcggc ttcttctgct cgacgagccg cttgccgggc
    12721 tcgccgcgac cgagcgcgag cgcatcggcc ggttgatcaa gcgcatctcc gccgacatcc
    12781 ccgtccttct ggtcgagcac gacatcgacc gggtgttcga gctcgccgac gcggtgacgg
    12841 tgatgaacaa cggcgaggtg ctgatcggcg gcacggtcga ggaggtgcgc tcggacccgc
    12901 gcgtgcagga ggtctatatc ggttccggca cggcggccct ggccggacgc gagatggtct
    12961 ccgtcgccga gccggaggtc ctcttgggtg cgcgcggcat caacacctat tacggcaaga
    13021 gccacgtgct cacggacgtc gatctcgacg tgcacaaggg cgagatcgtg gccctcttgg
    13081 gccgcaacgg ggcgggcaag tcgaccttcc tgaagacgct gatcggcatc gccccgccgg
    13141 cttccggcac gctggtcatg cagggcgagg atctggcggg gctgccgtcg gccgagatcg
    13201 cccggcgcgg catcggctac gtgccgcagg agcgggcact cttccagggc atgagcgtgc
    13261 gccacaacct cgagctcggc cggctcaagc gacgcaccgg tgccgggacc cactgggcgg
    13321 atgacgacat cctggccttc ttcccgcgcc tcgccgtgcg gctcgacacc gccgccgatc
    13381 gcctctcggg gggcgagcag cagatggcgg cggtggcccg tgcgctggtc ggcgacacga
    13441 agctcctgct cctcgacgag cccttcgagg gcctgtcgcc ggcggtcacc gaggagctgt
    13501 tcgacgtgtt cgaccggctc cgccgcgagg tcgccatcct gatcgtcgac cacaatctcg
    13561 atctggtcct gaacctcgcc gaccgggcct atgtgctcga gcgcggccag gtcatccacg
    13621 aaggcccgtc gcgcccgctc gcccacgact acgacctgcg ccgcgagatc ctctggcttt
    13681 gaggttcccg gcatcgatcg gggcggtgcg ggagccgggg cgccaaccgc gtgctcgatg
    13741 aatggtcagg caacccgtcc gcgatcgaac aagatggctc caggtcgagg gtcatccgag
    13801 cccggacgac cctcgatccc ggggatcgcc tattgggcca cgccggcgat ggagcggatc
    13861 atctcgagga atggctcgcg ttcattcgcc gcggcggctt cgtccaccgc gaagacctcg
    13921 acatccgcga gctcgggcag cgccagatac ttcgagacct cgatgtcgct ccggctgggc
    13981 cggcggaagc cgttgaccag cagggccgtc tgcgcctcgg cggagagcag catgtcgacg
    14041 gcctggcacg cgacatcccc ggccggcgcg cctgcgacca gacccagata ctccggggtg
    14101 acgaaggtgc cttcggacgg atagacgagc ttcagctcgt cctggccacc gtcgatatag
    14161 gcgtaggcgg tggcctcgaa tgtcaggccg atcgggtatt cgcccatggc cacgccgcgc
    14221 ggcaccgccg aggaggagcc ggtgacgacg acgttgccgg ccaatgcctg gaaagcggcc
    14281 tcgtcgagca gctccctggc acccaagagg atggtgtatc cggtggagct gttggccggg
    14341 tccggcacgg tgatcttccc ctggtagacc ggatcggcga ggtcgctcca ggtttcgggc
    14401 atcggcaggt cgccgaggag atcctcgttg accatgatca cggagacgtg gacgttggtc
    14461 ggcaaaaagc ggttgccggg gtagtgcagc ccctccggaa tggctgccag cgctggcgaa
    14521 tcgtagctct cgaacaggct ctcgaaagcg cccagcgtat tggcgctgga tgaccagaag
    14581 atgtcggcct gcaccttgtc cgcctcggat tcgatccgcc gcaggagaac gccgctgccg
    14641 ccggtgatcg cattgagctg gacatccggc atggcggtgc gtgccacctc ggtgatcgcc
    14701 tcgatcgact ggaccgggtt cgacgtgtag atattgactt cctgagccag cgccggtgct
    14761 gccaccatga gagcgaggcc taccacgctc cctgccgcca ccagccgtcc gtgcttcgtc
    14821 atctgaaacc tcccattgag ctgccacggt gaaagaacgc tctgcccggc agcacggcag
    14881 atgccatcgg ctcgatcacg attccgaacg gggcttgccg accccgaaga gatccattcg
    14941 gaagaccttg atcgctatca tgatcggcag aagaatgacg ccgatgagcg tcagtccaca
    15001 cgccgacgcg taggccatca gggcactgtc gagcaggcgg aagatctgga tcggcaaggt
    15061 ctcgcgcccg ccggagtaga cgaggatcga agcgctcagc tcggccaccg aggtggtcca
    15121 tgtcaggacc gccgccgcgg cgatcgccgg cgccatcatc ggcagcacca cacgcacgaa
    15181 ggtccgaaac ggtggcgagc cgaggctgat cgacgcttcc tcgatcgagt tgggcacatt
    15241 gtagagcgtc gaggaggcat tgcgcatgcc gaacggcagg cgtcgcacga cataggcgag
    15301 gacgatgatc gacgcggtcc cggtgagtgg cagccagccg gtgtggaacg agatgatgag
    15361 gccgatgccg agaacggtcc cggagagcgc caggggcacg acggagatgt agtcgaggag
    15421 cggtgtgatc aggttgcgct tcttgacgat catgtagctc acgaccgtgc tgaagctgat
    15481 gccgatgcag gtggcgagcc ccgcatagac aagcgagttc accagcggtt gcggcgccgt
    15541 gacgaagacg cgctccatgt tctcgagcgt ccaggtgccc cattgcatca ccggcccgcg
    15601 cgccctggtg aaggcgccca ggatgacgga gatcatgggc agcatggaga tcagcaccac
    15661 ggtgccggcg gcgagcgcga tcaacacgcc ctcgaggccg cgcagcgcgc tcggcggggc
    15721 accccgcccc tgcacgacct cgtagcggcc gcgcgcgacg atccagcgat tgatgaacag
    15781 gaccaccatg acgatgagga tcgagacgct cgccagcgtg ctctgcatca ccgggttgac
    15841 gccgccctcg gacacggcgg cccgataggt catcaccgac aggagctgca cctggtgccc
    15901 gagaatgatg gagatcgcga agttgccgat caccatcgtg aagaccagca aggcgcccga
    15961 gaggatggcc ggggcgatga ccggcaccat caccttcagc cgcgcgcgcg ctggcgagct
    16021 gccgaggctt tgcgccgcct cctccaggtg ggcgtcgaag ccggaaatgg ctgccacggt
    16081 gccgatgtag acataggtat agtagatgaa ggtcatcgac gtgacgaggc cgaaccagcc
    16141 gtagaagctg ggcaggatga tgccgtattc gcgaaagaac cgggtgatca ggccgttgtt
    16201 gcccagcatc atcagccagg tctgggcagc gattacctcg ggtatgacga gggtgatgag
    16261 cggcaggaac acgatcagcg actttccggg gaaccggaat cgcgcggtga cgtaggcgag
    16321 cggcacgccg accagcgtgc acgccaccgt cacgatcgct gccagcatca acgagttggc
    16381 gatcgcctcg ccgtagcgct gctcggacag gatctgcgac cagccgccgc cattgctgaa
    16441 gctcgacgtg acgatgttga tgatgggata gacgatgaaa acgaacatta tgacgagcgc
    16501 agcgacggac agccagaacc agaccgatcg aacatcgatc atcctgttca tcgcgcgagc
    16561 accagcgcct tctcgggatt gaccgatacc gacacatgct gaccggcgga aaatctatcg
    16621 tggtcttcgc cgtgcaggtc gaccgcgagc acgctgccgt cgccgagcct gaccttgtac
    16681 gccgtcctgg ccccgagata ctgccgaagg atgacgctgc ccggcagcga cgatgccgcc
    16741 ccgtccgtcg tcgggctgtc gtccattccc agatcctcgg gccgcaggac cagcacgccc
    16801 tcgccattcg gcaccggccc ggcgcagcgc gcccgcagga cgacgtcgcc gaacctgacg
    16861 gtcgggcggg tcgcatccgc accctgaccg agcgtgacag ggatccggtt cgccgccccg
    16921 acgaaatccg ccacgaaggt cgtggcgggc gtcgcgtaga tctccttcgg ggagccgatc
    16981 tgctcgatcc gccccttgtt catcacgccg atccggtcgg acatcgacag ggattcttcc
    17041 tggtcgtggg tcacgaagac cgtcgtgatc cgggcttcgc gctgcaactc catgatcgtc
    17101 tcgcggacct gcaggcgcag tcgcgcatcg agattggaga gcggctcgtc catcagcagc
    17161 acttgcggct cgatcaccag ggcccgcgcc agagccacgc gctggcgctg gccacccgac
    17221 agcgccgcgg gcttgcggga gcgaaagggg ccgagatcga cccgctcgag ggccttcatc
    17281 acccggccct cgagcacggc cccccgttcg ccgcgggcgc gcaggccata ggccacgttc
    17341 tcgaagacgg tcttgtccgg aaaaagcgcg tagtcctgga agaccatgcc gatgtcgcgc
    17401 ttgtgggcag gcagacgggt gacgtccacg tcgccgaaga ggatgcggcc gcgcgcggcc
    17461 ggaacaaagc cggcgatcgt tcgaagcagc gtggtcttgc cgcagccgga gggcccgagc
    17521 agggtgaaga agttgccttc gtcgatgcgc aggtcgagat cggcaatgac atccgcctgt
    17581 ccgtagctca ccgccagccc ggagatgctg atgctcatgt gccggccctc ctcttcgccg
    17641 cgtcctcgcg ccccagcagc tccagtgcca ccgcatgcat ggcggggtcg cccgccgcca
    17701 gcacgcgagg gccgctgtcg atcgtcaatg gcgcgccggc ccaatcggtg atgcgcccac
    17761 ccgcccccga aacgatggga gcgaatgcgg cgatatccca gatcttcagc cccgaatcga
    17821 tcgacaggtc gatgcgtcct tgcgccagac gcccgtaagc cagcgccgcc gccccgtaga
    17881 tccgccacgc cgtgcgtgat cgcaaccgct cgaaggcctg gatatcgccc ggcggaaaga
    17941 aatccgggct gctcgccgac aggatggcgc cggcgagctc ggcgcagggg cgggttttga
    18001 ggacattccc gttgaggcgt gtcggctccc cggcgacacc gacgaagcgc gcgcccaggg
    18061 cggggaagtc catgacgccg atcaccggat cgccatcgac gcagcatgcg atcagcgagc
    18121 cgaagaccgg aatgcccgcc atgaaggcgg cggtgccgtc gatcgggtcg aggacccaga
    18181 catggctgca accgcgaccg tgctcgccat gctcctcgcc gatgacgccg tggtcgggaa
    18241 agcgcctgga gatgatctcc cgcagacgcg cctcgacggc ggcatcggtc gcggtgacga
    18301 agctccgatc ttccttgagc gtggcgtcgc catgccggaa agcgccgtcg acgaggctgc
    18361 ggcctatcat ctcgcggctc gtctcggcga gctcgattgc catgcgcaag caggtgacga
    18421 gattggccgt ggatgccaaa ttacagtcct ttgccgagta ttccgttcat gcactatcgg
    18481 ctccggttta tctgtcaagg cggtacagcg gcatccgtcc gacccggaac gggctgcatg
    18541 acaggcaatc gccacccgcg atcgagcttt gcgcgccttc atgtgcgcaa aacgaggttg
    18601 ccgatgcagc agcgatggac tcattgcgcg gatccggcgt ctggtcacgg aatgcgaacc
    18661 ggagcaggag gcggagcgcc ggcaattgca gcaatctcag caggagctcg agggcctgcg
    18721 cgccggcccg ccccgaaccg ccgcagatgc tcctcctcga ggacatctcg cccgaggtcc
    18781 ctgccgtcga cctcggtcgg ggctgcccct tcgcctcggc ctgctcgaac caagccggtc
    18841 tcgtcccagg ctcgcgcggc attctcgccc gattccggat tgccgcgccg cgaggcaggc
    18901 agcgcacccc cgggtctcat tcagacgcga gcgcggcggt tccttctctt tcctcgatga
    18961 tcgatcggac gtcggtcacc gtcgagtgct cgacggggga ggcgctgttg ccgaaggcgt
    19021 tgcgcagata ggtcaggagc gccgccactt cgtcatcgtc cagcttccag cctaaggccg
    19081 gcatcgccag cggcgtgggc tttgcctccg tcgacggctt ctccgcaccg ccgagcacga
    19141 tctggatggc gctgtgcggc tcctcctgct gcaggacgct gctgccggcg agggccggga
    19201 agacgccttc ctggccctcc ccgctcatca ggtgacagcc ggtgcagttg tcgacgaaga
    19261 gcgcctcacc ctcggccatg aggagttcgt cgaggtcgcg gacgtcgccc ggctcggcca
    19321 cctcctccgc tgccggcagg tcctcgaggt agaccgccat ggcccgcagg tcctcccggg
    19381 tcaggtgctg ggtcgagcgt tcgacgacct ccgccatcgg gccgccggcg gaggagaagc
    19441 ggttgctgcc cgtcttcaga tattcgacga tgtcctcgac cgaccactcg cccagcccct
    19501 cgcgcaggtc gcccgtcagg tccggcgcga accagccctc gccgaagccg ccctcgaagg
    19561 cctcgtccag ctcgaccgcg cccgccaggt tcttgggcga gtggcaggca gcgcaatggc
    19621 cgagcccctc cacgaggtag gcgcctctgt tccactccgc ggacttcccg ggaaccggcg
    19681 tgaagacgcc ttcctcgaag aagagcgcgt tccagcccgc gagcaaggcg cgccagctca
    19741 acggccaggg cagctccggc gggtcgacct cctggcgcac cggctccagc gtgtcgagcc
    19801 aagccttgat ggcgcgaatg tcgtcgcgcg tcacctttgt gaagaagggg tatggaaagg
    19861 ccggatagag ccgcttgcca cccggcgcca tgccctgggt catggcgcgc tcgaactcgg
    19921 catccgacca gccgccgatg cccgtttccc gatcgggggt gatgttcgcc gagtagagcg
    19981 tgccgaacgg tgtcgggatc gggcggttgc cggcgaaagg ctcgccgcct tcggccgtat
    20041 ggcaggcgac gcagtcgccg acccgcgcga gatagcggcc tctctcgatg tccgagaagc
    20101 ccgcctcctg cgccaggccg gcggccgtgc ccgtcagcac ggcggcgccc gccagaacgg
    20161 tgagcccggc cctcatctca cgcatccacc agcgggccgg ggtttttcag gtagcgggtc
    20221 ttgatcgcct cggccgccca gtaggtgagc gcgcccaccg tgccggtcgg gttgtagccc
    20281 gcgttctggg gaaacacgca ggcccccatc acgaagaggt tggggacgtc ccagtgctgg
    20341 aggtagcggt tgacgacgct ggtggacggg tcgtcgccca tgaccgcacc gcccgtgttg
    20401 tgcgtcgtct ggtaggggac gatcgaatag ggcccctggc gctggttgac ggtgtaggag
    20461 cgcggccgca tggccctggc gatctcctcc gcccgggcgg tcacgaagcg ggacatgcgc
    20521 cgctcgttgt cgtggaagtc gaaggtgagg cgcatgagcg gatcgccgta ggcgtctctg
    20581 taggtcggat cgagatcgag atgcgcgtcg ttgtagctca tcacgctgcc gtgcacgctg
    20641 agcgaggcgg agcgcaggta gttctcggcc atcgcctgct tccacgcaga gccccattcg
    20701 ggcgtgccct gggggagctg gtgctggagg atcggccggc cgccggtggt ccagagggcg
    20761 acataggcgc cgccgatgaa gccgtgcggc ccgtggtcga aattgtcgcc gttgaagtcg
    20821 tcgatcgcct gcccgagggc gccggcgccc atgaacgggt tgaggttctc ctcgacgaag
    20881 acgtccaccc cggacaccat ctggtaggcg tagttgcgac cgagggtccc ggaccgcgtt
    20941 ttcgggtcgt acacctcgcc gatgccggag agcagcagga ggtggacgtt gtgggtggcg
    21001 taggcggaga ggatcaccag gtccgccggc tgctcgtgct cctcgccgga gctgtcgacg
    21061 tagatcgcgc ccgtggcgcg cttgccgctg cggtcgagct tgatgcgcgt gacgttgcag
    21121 ttggttctca agctgaaatt gctgcggttc aggaggaccg gcaggatcgt cgtctgcggc
    21181 gtcgacttgg agtagttggc gcaaccgaac cattcgcaat agccgcagta ggtacatttg
    21241 cccatctgca cgccgagcgg gtttcggtag ggccgcgagg tgttggaggc cggcgcgggg
    21301 aacgggtgga agcccgcctc gcgtgccgcc ttgtcgaaca gcagcgtgcc gaggctgcgc
    21361 tcgagcggcg ggttggggta gtcgcgcgag cgcggcccct cgaaggggtt gccctgcgcc
    21421 gtgatctcgc cgttcaggtt gccggcgacg cccgacgtgc cgcagagata ctcgaagcgg
    21481 tcgaaatgcg gctcgagctc ctcgtaggtc acgccccagt cctggatcgt catcccctcc
    21541 ggaatgaagt cggccccgta gcgctcctcg acatggctgc gctgccggaa gtcgctcggc
    21601 aggaagcgcc agtgctggcc attccagtgc acgccggcgc cgccgacgcc ggtgcccggg
    21661 ttgaacgagc ccagccggcg catcggcaga gcggtctgcg cgtggttgtt gcggaaggtg
    21721 atggtctgcc gcgccggctc ctggaacatc tcgtggcgga cggcgtaacg cagctcgtcc
    21781 tggatctgcg gcatggcgaa gtcggggacg gtgtcgcgga actgaccgcg ctcgagcgcc
    21841 aggacctcga gtccggcctg ggccagctcc tcgcccagga ttgcggcggt ccagccgaac
    21901 ccgaccagga gcacgtcgac gggtgggagc ttggtcggca tcggtccctc cgccctcaga
    21961 tgaagccgtc gcggccgagg atgccgactg gcggcagcgg gaaaggctcg ccgtgcctgc
    22021 cgatgaaccg ggtgtagtcg tagcgtgggc cgggaaagcc gatgagccgc cagccgacga
    22081 agccttgatt gccgccatac atggggtcgc tgaggaagcc ctcgacagtg ttggctcgca
    22141 gcatcttgaa gaaactcctg ctgtcgacat cgccgagctc gggctcaccg gcctccagtc
    22201 cgcggagcac ctcgtcctgt tcctccgcgt ccagatcagc gaacacgcgg ccgccgaaaa
    22261 agtcccgaca gtgctgatcg accgcccgga tggcggcgct gtagagctgg gcgggtgtca
    22321 gtggcagctg gtagccctgc tcgtctgtcc cgtctttgaa gggtccttgc atgtaccagc
    22381 gggcggcatc gccgaaatcg cccgcgagct gggattcgat gaactgggtc acgcccgctt
    22441 cccgtgcacc ggcccccgtg tcgtccgtcg ggatgatccg ggcggtggcc gcgtcgacga
    22501 aagctcgttc ggcgtccgtg aaatgcccgt cggcgccggg ccgtcgggtc agtgccggaa
    22561 ccatgctcgc ggaggccgcg gagagaagcg cgccaatcgg cgtcagctag ctggaggtga
    22621 cgcagaggac tttcaacacg cggcgtctgt tcatcgaaca ctctgaccag tttgcccggg
    22681 ccagacaaaa accgcgccca ttcccctcgg ttccgttcgg cggctcgttg ggggtcgttt
    22741 ttcgatccaa ggtcacggag ccgacgtcgt cgagcgcgaa gtgacgaccg ataggcacca
    22801 cgggctaaac caaacgtgcg cttgccgatt gcatcaagct ggatgtctgt tctcgtcctg
    22861 gaaccttcgg cgtagctgtc gattcaaggg ttccttggcg tggaacagag acgtcgatcg
    22921 accacgcgat accgcgtctg cacgtccagc cgaaccggac cagtagttgg gctcgtcact
    22981 gtgccaaccc gacggcctca cccgcgtcga tcaagtgctg tcaggggtaa atgcccaccg
    23041 agatgaccac cttcgagctt cggctgccat gaacgagctc gatgccattg tcgccatcgg
    23101 cgcgggcgcc gtgttgctgc tcggcgtgac atccggttgc attcgcaatc ggctctggat
    23161 ctccgagccc gccgtctgcc tcgcggtggg cgtggcgctc agtccgctcc agttcgagtt
    23221 cctcggggta gcgccgggct cggatgaata tctgacgctc gtccagcagg tcgctcgcct
    23281 caccctggcg atctcggtga tgggcgccgc cttgcgcctg ccgcaggact acgtccgtcg
    23341 ccattggcgc gagctgacgc tgattctcgg cttggggctg ccgatgatgt gcctgagcgg
    23401 cgcttttctc gcctacctca tgctctcggt gcccctgctc acggccttgc tgatcggcag
    23461 cgtgctcgcc cctaccgacc ccgtcctgtc ggattcgatc gtcgccggca ggatcgcgga
    23521 tggccacatt cccgcgcgca tgcgcaacag cctcactgcg gagagcgggg ccaatgacgg
    23581 gctt
//
LOCUS       DAOTFI010000039        23254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2593_length_23254_cov_6.101685, whole genome shotgun sequence.
ACCESSION   DAOTFI010000039 DAOTFI010000000
VERSION     DAOTFI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 23254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23254
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2593_length_23254_cov_6.101685"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="P5558_05690"
     CDS             complement(<1..227)
                     /locus_tag="P5558_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23833.1"
                     /translation="MQPATSFWPTALSAHAHEVDLLLIGLAGLCALLTLPVFVALGWF
                     AIRYRRGSAASRTPRRRRNWKLEFAWALIPFG"
     gene            complement(239..1018)
                     /locus_tag="P5558_05695"
     CDS             complement(239..1018)
                     /locus_tag="P5558_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRY23834.1"
                     /translation="MIGLLIAPALALAALLGATPRAVAAFDPFASAAIDDRLGARLPM
                     RLALWDEAGREVRLGDYLADKPLLLAPVDLDCQNICGVTLGGLLDALAGLDRGPGEDF
                     ELLLVSIDQQEGPAQGAAAAATYREQFAPAARAHFLTGDARVLLDAIGFRYAHDPATG
                     QFAHPAAVAVVTPEGRLARWLYGYPFAAFDLRLALSEAAGGAIGSLADRLWLLCFRYD
                     PTTGAYTARVLGLLQAGGILTTLLLGGGIALALRRERRRRR"
     gene            complement(1015..1443)
                     /locus_tag="P5558_05700"
     CDS             complement(1015..1443)
                     /locus_tag="P5558_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23835.1"
                     /translation="MAERPGQRVQGLGHEPSDVSVRGVLLTAFGGLLALAVAGVAIRG
                     LEALYGLHDAPPPSALDQRSLEPPPHRLQTDPAEDLAAYVAREEAILDSYAWIDRKAG
                     IARIPIDRAIEMLAERGWPEPAEGEGRPPQPVTGRGKADR"
     gene            complement(1436..2560)
                     /locus_tag="P5558_05705"
     CDS             complement(1436..2560)
                     /locus_tag="P5558_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23836.1"
                     /translation="MTPERSLPLFRRVAALSASFGMVVCLFFALADLAAFWRAYLVAF
                     VAWHTVPLGCLALLLTYHVAGGPWGRMLGEALEAGARTLPLVALLGLPLLFDLAALFD
                     WAQPAYGAHEELLRNKSLYLNPPFFVARSLFYLGFWAVLAWLITDRRVPLADRPRRQG
                     AAALGAIVLALTASFASIDWVMSLDARFNSSTFGLIVIGGHLNAGLSFATLITLASAR
                     VDRRAQVLAEAPMVGLGGLLQSAILLWAYLVFMEYLVVWSGDLPQNAAWFLQRASGGW
                     AALLAIIACGHFVLPFALLLSSRLRGDWRVVSGLAVLILATQLLYLVWQTAPAWGAVA
                     PPPLAIAGALLALAGLWLLMVTANLAARRELLVPREKIGG"
     gene            complement(2557..3096)
                     /locus_tag="P5558_05710"
     CDS             complement(2557..3096)
                     /locus_tag="P5558_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY23837.1"
                     /translation="MSRRFPCLLSLVGVLLGGCDQSMDDQAKYEAFEAADLFPDGKVS
                     QPPVPGTIARGELAWRAALTERPPLDRPLIERGRERFTVFCSPCHGHSGDGDGMIVQR
                     GFPRPPDLHAERLRSAPTEHFVDVITNGYGVMFSYAARVPPADRWAIAAYIRALQLSG
                     HAEVASLPPADAARLEQAR"
     gene            complement(3093..3641)
                     /locus_tag="P5558_05715"
     CDS             complement(3093..3641)
                     /locus_tag="P5558_05715"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRY23838.1"
                     /translation="MSEREQPDRLYGLLAVFTDPDALLCATRQMRAAGYRELEGHTPF
                     PVEGLDDALGFEESRLPFIALAGGILGGSGGLLMQWYLNAYDYPINVGGRPLDAWPAF
                     AVPAFETSVLGAVLAVVGAMLWLNGLPRLHHPLFDAPSYRRVSLDRFCLSVRREDPCF
                     DREATARLLEGLGASVVEEVPA"
     gene            complement(3634..5004)
                     /gene="nrfD"
                     /locus_tag="P5558_05720"
     CDS             complement(3634..5004)
                     /gene="nrfD"
                     /locus_tag="P5558_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRY23839.1"
                     /translation="MDTTAQDVRRRRSDILPRHLSYRAVGRQVALIPLDYPHPRQWRI
                     GFAVCSVFVVVWAVSVLWLFWGGVGVWGIQMPVPWGLAIANYVWWIGMGHAGTLISAL
                     LLLMNQGWRNSLNRFAEAMTVFAVLMAGMYPILHLGRPWLFYWLIPYPDTMTVWPQFK
                     SPLEWDVFAVGTYLVVSVTFWYVGLIPDLGSMRDRATRRAPQVLYGLLALGWRGSARH
                     WKRWQVSYWICAGIGVPLVVSVHSGVSLLFAVGLEPGWHSTIFPVYFVLGAVFEGFAV
                     VLLISITLRATFAFYDLVTPRHLDILAKLVLATGLMTSYGYVFETFHAWYSGDPFERQ
                     TMLDRLWGDYAWAYWATIACNVLAIQPLWFPAVRRRVWLLAVIAGLVTFGMWTERFML
                     LTTALYRDFLPSAWQVYWPSFWDWSLFAGTIGLFFFLFFLFVRLLPMISMFEVQEVLS
                     EEGTHE"
     gene            complement(5008..7779)
                     /locus_tag="P5558_05725"
     CDS             complement(5008..7779)
                     /locus_tag="P5558_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY23840.1"
                     /translation="MAASLALAGLGGCDPVQPEERALPHVGMPEFGAAGEPRLYAAAS
                     LLNGYAIPLLVETVDGRPIKVEGNPEHPLTRGGTDLFAQAAVLDLYDPDRSRTTAYLG
                     EVRPWPEAQRDLLVKAQELERRGGEGLAILSGTITSPTTVRQLRRLQDRFPALRQFRH
                     EPVGDQRRQAASLLAFGRPLDQRYRLERARAVLALEADPLGPGPAQIANARGFSDARR
                     AALEEGRAMRLLAVESTPTLTGAKATERWPADSALVEAVVVALARQLGIGPAEAVDLP
                     LGLRSWLLAASAELEQAGKAALVALGHHLPPRLQALGYRINERLGALHHTIELTEPAA
                     WQPDGVGDLEDLAEALRGGSVDTLLMLDTNPLYTAPDDLDLAALLRQVPLTVHLGQYQ
                     DETAAASRWHIPMCHAFEGWRDARAVDGTATILQPTIRPLHASHTEAELLATLAGDPD
                     VTPYDAVRTTWRDMLGEEGFEARWAKLLSDGFAAETAAARVTDAPRPIEVAPPPATAE
                     AGTLEAVFRPDPSIWDGRFANNPWLQELPKPLSKLTWDNVAAISPALAAAQRLANGDR
                     VRISAGERLLEAPVWILPGQAERTVTLYLGYGRKLAGRIGNGVGYDAYRLRREADPWR
                     LDDVTLQAVGAAAALATTQLHRTMAGHDLIRQTTPEAVRAGTPVAPPFEEPSLYPDWP
                     YAEESWGMVIDLDTCIGCNACVVACMAENNVPVVGKEQVAMGREMHWLRVDTYYEGAP
                     GNPETHFQPVPCMHCEQAPCEVGCPVNATVHGPEGLNQMVYNRCVGTRTCASYCPYKV
                     RHFNFFDYAGGESEGRVPQRNPEVTVRARGVMEKCTYCVQRISRARIAAKTDERAIRD
                     GEVVTACQGACPTRAIVFGDLNDPDSAIRRAKASPRNYALLEELGVRPRTTYLAKVAP
                     SGRRGSEPA"
     gene            complement(7959..8573)
                     /locus_tag="P5558_05730"
     CDS             complement(7959..8573)
                     /locus_tag="P5558_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY23841.1"
                     /translation="MGLIIISGLVAGGLLLASGLVRSDYLTGVGVAMEQPVPFSHKHH
                     TGELGIDCRYCHDQVETAASAGYPPSHTCMTCHSQLWSNAAALAPVRQSFTDDRPLRW
                     NRIHDLPDYVYFNHSIHVARGVGCAECHGPVDRMTRIYQARPFYMRFCLDCHRDPAPH
                     LRPPEQVFNLDWQPPPDRAAFGRRRMAELDIQPRHLDSCYICHR"
     gene            complement(8775..10289)
                     /locus_tag="P5558_05735"
     CDS             complement(8775..10289)
                     /locus_tag="P5558_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRY23842.1"
                     /translation="MTLPESEPARRLPSAGVLKAARTHMKQRGGGCSIRPHAGPILRV
                     GRNCGTIAAARRAAVIVDAARYFAALHSVLRKAERSVLILGWDFDAGIALHPDSRESE
                     QLGNFLRALVEERPTLEVRVLVWNLATVHSPGATAPLLFGASWHEHPRIRVALDSLHP
                     IYAAQHQKIVCVDSIAFVGGIDLTVERWDTTRHAPNDGGRTSPDGGAYRPVHDLQWMI
                     DGAAAQAVCRIARERWRVVTGETITSSTSSLDPWPSDVEPDFENVPMAVARTAPAYNG
                     APEVREVETLVLDALVAAKSRIYIETQYFAEPRIADVLARHLAREGGPEIVVVVSRAA
                     RGVIEKWVMGNNRDRLVRRLRRADRHGRFRAYCPVVRGSSQVEILVHSKLLVVDDQFL
                     KIGSSNLNRRSMGLDAECDMALEAGEQRTAAGIRAVRNRVIAEHLGKTAGAFAEAARS
                     TGSIIAAIERLNDGPRRLEGLGIRDRGPTHVMPGTRILDPLTPFRLSSLLWRRH"
     gene            complement(10293..12392)
                     /locus_tag="P5558_05740"
     CDS             complement(10293..12392)
                     /locus_tag="P5558_05740"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRY23843.1"
                     /translation="MSEKRDAIEVADQKLQRGDGGEYHQIAGGDGEVLTTNQGVPVSD
                     DQNSLRIGPRGPLAMEDFHFREKLFHFDHERIPERVVHARGMGAHGYFETYESLADVT
                     RADLFQRKGEKTEVFARFSTVAGNKGSADIARDVRGFAVKFYTREGNWDLVGNNIPVF
                     FIQDALKFPDLVHAAKQEPDRGFPQAQTAHDTFWDFISLTPESINMALWIMSDRTIPR
                     SLRFMEGFGVHTFRLVDAEGKSTFVKFHWKPKSGMQSVVWNEALKINGADPDYHRRDL
                     WDAIQMGDYPEWEMGLQLFDEAFAEQFDFDVLDPTKIIPEEVLPIRRVGRMVLDRVVD
                     NFFAETEQVAFCTQNVVPGIDFTDDPLLQGRNFSYLDTQLKRLGSPNFHKIPVNSPRG
                     CPFANFQQDGHMRMQVPKGRALYQPNSFGGPREAPRTGFRTYPAEVSGQKLRMRPESF
                     ADHYSQARQFYRSQTEVEQNHIVSAFTFELSKVETVAIRERMVAHLLTVDEDLGTKVA
                     AGLRLKSLPNAAPPAVEPRTDLARSPALSILLNGPRSFAGRKVGALVTDGVDAEILAA
                     LKTALEKEGAMLKLVAPMVGGVEASDGTWLEADEKIDGGPSVLFDAVAILPSEAGCAQ
                     LVDEATARDFVADAFAHLKFIAYSPAAEPLLRKAGILEALDEACIALDSSSDAQPFVE
                     ACRKLRHWPRETAVKQV"
     gene            complement(12432..14597)
                     /locus_tag="P5558_05745"
     CDS             complement(12432..14597)
                     /locus_tag="P5558_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY23844.1"
                     /translation="MGAIGKPVARVDGPAKVTGTATYAAEFHPENMAYAALVHGTIPR
                     GWIKSIATDAAESVPGVIAVVTHLNAPRLPWKPVDAAPVDPGEGEQLRPLHSDEILFA
                     GQPIALVIAESQETARAAAALVSFTCVPSDGAGLVFDRARARPPSDGTRESYPAEWER
                     GDAEGAFASAPRRIEGSYLQPRLFHNAMEPHATIAAWDDEGKLTLWDKSQWVDNVRDQ
                     ITRNFGLAEDEVRVISPFVGGAFGSALRCWPHVTMAAMAARIARRPVRLELTRRELYT
                     AVGYRPYTLQDVKLAADEDGRLRSVIQTAYGLTSAYEDYGEEVLDPVGMLYDTMSART
                     VYRLVELDTNTPTPMRGPGAVTGVFALEMAIDEMADAMDMDPLEFRLKNYAERDLKKD
                     LPFSSKALRSCYETAALRFGWEKRDPRRRAMHDGNMLVGYGMATAVWPAMQAPASVRI
                     RLFANGTAVVRTAASDMGPGTYTSMTQVAADRLDLPVERVTFELGDSSLPMAPVHGGS
                     MTMASVGNAVAAGCKKLRDDIARLDGAEDGEHPVETLMRLRRDSFEVDGAAEPGAEAG
                     THSSYAFGAVFAEVRVDPDFGTVRVPRLVGAYDGGVIVNPRTARSQAIGGMVQGIGMA
                     LMEAGEWDDRLGRVMNANLAEYLVPVHADVEELEAVFVESEDRIFNPLGVKGIAEIAL
                     CGVAPAIANAVWHATGLRIRELPLTPDRLLMAEQAARTS"
     gene            complement(14597..15586)
                     /locus_tag="P5558_05750"
     CDS             complement(14597..15586)
                     /locus_tag="P5558_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRY23845.1"
                     /translation="MSGLAYARAASLESATEVIGSHRAAEVIAGGTDLLQRLEEHVLP
                     PPLVVDINGVEGLRAIEVDDHEIRLGALVRLNQVIESAPVRDAFPAIVEALIVTASPQ
                     VRNLATVGGNPLQKTRCLYFRDQSTPCNKREPGSGCGALEGRNRINAILGGSEHCIAT
                     HPSDFAVAMAALDATVLVVGARGERRLPFTELHRLPGDTPHLEHNLAPDEVIEGYRLT
                     RTPLARHSHYLKVRDRGEFEWAICSAAVALELDHGGRIADARVAVGGVATKPWRLGQV
                     ETALQGRQATAEAFAEAAALSVQNAVAHGDNAYKLALLPRTIARALTELRERL"
     gene            complement(15583..16050)
                     /locus_tag="P5558_05755"
     CDS             complement(15583..16050)
                     /locus_tag="P5558_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY23846.1"
                     /translation="MRVTLEVNGREHALDVQARTTLLDALRDRLHLNGTKKGCALGQC
                     GACTVLMDGERVNACLVLAVMAQGRHITTIEGLRGGDGLLHPLQRAFLDHDGFQCGYC
                     TPGQIMSGVGCIEEGHAGSRAEIRQYMSGNICRCGAYVNIVEAIFETAVGTRP"
     gene            complement(16335..17144)
                     /locus_tag="P5558_05760"
     CDS             complement(16335..17144)
                     /locus_tag="P5558_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRY23847.1"
                     /translation="MAPRTFWKGYLKLSLVSCRVAMVPASSDREKIRFRTLNRETGNT
                     ITSRYVDAETGTPVTDDALAKAYEVAEDRLVILEDEELESVALESTRTIDIDCFVERD
                     SIGWIWLDTPHYLFPDDRVGEEAFAVIREAMERTGTVAISRVVLYRRERALMLEPRER
                     GIVAWTLRYGDEVRSAEAAFAKVEEQGPDEKLMGLLGELIEARTRPWAPTLVTDPVEG
                     RLAGLVAAKQRKRKPAPKRQRTAAPVAASGDNVVSIFDALRRSLAEEKKRR"
     gene            complement(17318..17407)
                     /locus_tag="P5558_05765"
     tRNA            complement(17318..17407)
                     /locus_tag="P5558_05765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17371..17373),aa:Ser,seq:tga)
     gene            17593..18501
                     /locus_tag="P5558_05770"
     CDS             17593..18501
                     /locus_tag="P5558_05770"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY23848.1"
                     /translation="MSQVRDRLFGIVPPTTTAFDAAGEIDEAGIRAQVRFLLGAGVHG
                     LAAGGSTGEGHTLDHEEFVRLMETTVDEVAGRVPVVAGIIVDSTRDAIRRARSIAHLD
                     VVALQVTPVHYLFKPSDDAMVEHFRRLAGETGKPILIYNVVPWVYLSPQLLYRIFREV
                     PGVVGVKQSAGDLKRVADLLFITEPEDLVFTAVDALLYPAFMLGAHGTIAALPTAAPH
                     ACVALWDACQAGDHARAKELHLQLLRVWNAIEADNLPACTKYALTLQGCAAGLPRPPM
                     HAPSETQKKNIREALQAMGVSLQTAA"
     gene            18534..19514
                     /locus_tag="P5558_05775"
     CDS             18534..19514
                     /locus_tag="P5558_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY23849.1"
                     /translation="MNERAGIRLAAAGLVAAVTLATTSAALAAWPERPVTLIVPWSAG
                     GGTDATGRIVASMLEERLGQPFNVVNRTGGGGVVGHTDIANAAPDGYTLGVITTELSM
                     FHWIGTSPLTFEDYSLFGLYNTDPTAIHVRADFEAADLGALLEMVDAAPGQHKASGAN
                     QGGAAHLGLAGLLQASGRAPDAIPWIPAEGAAPSLQLLVSDAIDVVSTTMPEVRSLVD
                     AGEVRTLAVMASERDPAFPDIPTVAEAIDVDWTTGAWRGIAGPAGMPEEVAEVLVPAL
                     EEIVASAEFKEFMAGRGFGVTWAAPDDFAAYLATADANFGAAMQAVGLAK"
     gene            19518..19979
                     /locus_tag="P5558_05780"
     CDS             19518..19979
                     /locus_tag="P5558_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY23850.1"
                     /translation="MRLADGLSGGVVVAGAALLLWTASGLPDMPGQRFGPATFPTLIG
                     IAMAAAGLVVLWRGRPERPLFSIGTLGHDRRVQVAVLWLLGGLVAMLVLWQPVGFPLL
                     AAVYSGGLMLILGVRPLPAVFWACAVAVVVHLVFTRLLLVPLPAGPLGFLL"
     gene            19989..21485
                     /locus_tag="P5558_05785"
     CDS             19989..21485
                     /locus_tag="P5558_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY23851.1"
                     /translation="MADLLAAWALVLDPEVLLVILAAAFFGLFVGAVPGLTATMATAL
                     LVPVTFFMAPLPAVAAIVACTAMAITAGDIPGALLRIPGTPASAAYTDEAYALTRAGQ
                     PELALGAGLVCAALGGLFGALVLVVAAPLLARIALGFSSFEYFWLAALGLTSAAFVSA
                     ADRAKGVVSLTLGLFIATIGLDITSGLPRFTFGSVELMGGVSFIPAMIGMFAVSEVLR
                     NITDGTSLGTVPKVNVGNPFRGLGAVLWRYRLNVLRGSALGTTVGALPGAGGDIAAWI
                     AYANAKRFSKTPEKFGQGHVEGIVEAGSANNSGLSSAWVPAMVFGIPGDAITAIAIGV
                     LFMKGLNPGPRIFVEQPAMLYAIFITFFLANLLLIPLGWLAIRAARPILAVPRNLLLP
                     LILIFCVVGAFAINNTVFGIAVMLVLGVVAWLMESSGYPIAPVILGIVIGPLLEQNFM
                     ISMMIGDGDLLAFFARPIAAVLGVITVAVWLAPLALRLARGWRPGPHP"
     gene            21512..22903
                     /locus_tag="P5558_05790"
     CDS             21512..22903
                     /locus_tag="P5558_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRY23852.1"
                     /translation="MDTPSTLENPAIDGLRALFGERCSTRPAEREQHGRGESYHPCRA
                     PDAVCWPGSTDEVVAAVEICRRHRFPIVPFGVGTSLEGHVAALEGGVALDLSRMNRIL
                     MVRPEDMDVTVEAGVTRKQLDQELRDSGLFFPIDPGADATLGGMAATRASGTNAVRYG
                     TMRENVLALTVVLADGRVVKTANRARKSSAGYDLTRLFVGSEGTLGVITEVTLRLHGR
                     PEAVASAVCSFPSVDQAVATAMETVQAGIPVARMELLDGNVMEAVNRFSKLDYPATAT
                     LFLEFHGSAASVEEQAAMVGELAAGNDGSAFRWALRPEERNALWQARHDLHYATLAMR
                     PGAKVWGTDVCVPISALGECIREARRAAAEAPFFVTTLGHVGDGNFHMGYLIDMADPA
                     ELQAAERLNERLVRHAISLGGTCTGEHGVGYGKAKFMTLEHGDAAVSLMRQIKHMLDP
                     ENLFNPGKILPPA"
     gene            complement(22914..>23254)
                     /locus_tag="P5558_05795"
     CDS             complement(22914..>23254)
                     /locus_tag="P5558_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23853.1"
                     /translation="LAEGDADRRFAELYLDPTTGLVFDLLVIAETPEALRSDALIVTF
                     RDSTGSNLPGTFLEHDVALEPALGGALHAARARVMVVLPDGHDWATAEAMTFELQALD
                     QDFALTWTFP"
BASE COUNT         3689 a         7935 c         8018 g         3612 t
ORIGIN      
        1 ccgaagggga tcaacgccca ggcgaactcg agcttccagt tgcggcgccg ccggggcgtg
       61 cggctcgctg ccgagccgcg gcgatatctg atggcgaacc atcccagggc aacgaacacg
      121 ggcagcgtca gcagcgcgca cagcccggcc aggccgatca gcagcagatc gacctcgtgg
      181 gcgtgcgccg aaagcgccgt tggccaaaac gatgtggccg gttgcatgcc ggcgccgcct
      241 atcgccgccg gcgccgctcg cggcgcagcg cgagcgcgat gccgccgccc agcagcagcg
      301 tcgtcaggat cccgccggcc tgcaggagcc ccagcaccct ggcggtgtag gcgcccgtcg
      361 tcgggtcgta gcggaagcac agcagccaca ggcgatcggc gaggctgccg atggcgccgc
      421 cggccgcctc gctcagcgcc aggcgcagat cgaacgccgc gaagggatag ccatagagcc
      481 agcgggcgag acgcccttcg ggagtcacta cggcaaccgc cgccggatgg gcgaattgtc
      541 cggttgcggg atcatgggcg tagcggaagc cgattgcatc gagcagcacg cgcgcgtcgc
      601 cggtgaggaa gtgggcgcga gccgccggcg cgaactgctc acgataggtg gccgcggccg
      661 cggccccttg cgccggcccc tcctgctggt cgatgctcac caacagcagc tcgaaatcct
      721 cgcccggccc gcgatcgagc ccggccagag cgtcgaggag gccgccgagc gtcaccccgc
      781 agatgttctg gcaatcgagg tcgaccggcg ccaggagaag cggtttgtcg gccaggtagt
      841 cgccgagccg cacctctcgc cccgcctcgt cccataacgc cagacgcatg ggcaaacgcg
      901 cgccgagccg gtcgtcgatc gcggcgctgg cgaatggatc gaaagcggcg actgccctcg
      961 gcgtcgcgcc gagtagcgcg gccagggcca aggcgggggc gatcaacagg ccgatcatcg
     1021 gtccgccttg ccgcgtcccg tcaccggctg cgggggtcgc ccttcgcctt cggcgggctc
     1081 cggccagcca cgctcggcga gcatctcgat cgcccgatcg atcggaatgc gcgcgattcc
     1141 ggctttccgg tcgatccacg catagctgtc gaggatcgct tcttcgcggg cgacgtaggc
     1201 cgcaaggtcc tcggccggat cggtctggag tcggtggggc ggcggctcga gcgagcgctg
     1261 atcgagcgcg ctcggcggcg gcgcatcgtg caggccgtag agcgcctcca ggccgcggat
     1321 cgcgacgccg gcgacggcga gtgccagcag accgccgaac gccgtcagca ggaccccgcg
     1381 gacgctgacg tcgctgggct catggcccag cccttggacg cgctggccgg gccgctcagc
     1441 caccgatctt ctcccgcggt accagcagct cgcgacgggc cgcgagattg gccgtcacca
     1501 tgagcagcca gaggccggcg agcgcgagca acgcgccggc gatagcgagt ggcggcggcg
     1561 cgaccgctcc ccatgctggc gcggtctgcc agaccagata gagcagctga gtcgccaaga
     1621 tgagaacggc gagacccgac accacccgcc agtcgccgcg caggcgcgac gacagcagca
     1681 gggcgaacgg caggacgaag tggccgcacg cgatgatcgc gagcagcgcg gcccagccac
     1741 cgctggcccg ctgcagaaac caggcggcgt tctgcggcag atcgcccgac cacaccacca
     1801 gatactccat gaacacgagg taggcccaga gcaggatcgc cgactgcaac agccccccga
     1861 gcccgaccat aggtgcctcg gccaggacct gggcgcggcg gtcgacacgg gcggaggcga
     1921 gcgtgatcag cgtcgcgaag gacaagccgg cattcaggtg gccaccgatg acgatgaggc
     1981 cgaaggtcga ggaattgaag cgcgcgtcga gcgacatcac ccaatcgatc gaggcgaagg
     2041 acgcggtgag cgcgagcacg atggcgccga gcgcggcagc cccctggcgg cgcgggcggt
     2101 cggccagggg cacccggcga tctgttatca gccacgccag cacggcccag aagccgaggt
     2161 agaacaggct ccgcgcgacg aagaacggcg gattgagata gagggacttg ttgcgcagca
     2221 gctcctcgtg ggcaccgtag gcgggttgcg cccagtcgaa cagggcagcg aggtcgaaca
     2281 gcagtggcag gccgagcagc gccaccagcg gcagcgtgcg cgcgccggcc tccagcgcct
     2341 cgccgagcat ccggccccaa gggccgccgg cgacgtggta ggtcaggagc agcgcgaggc
     2401 agccgagcgg cacggtgtgc cacgccacaa acgccaccag gtaggcgcgc cagaaggcgg
     2461 cgagatcagc gagcgcgaag aagaggcata cgaccatgcc gaacgatgcc gagagcgcgg
     2521 ccacgcgcct gaacaacggc agcgatcgtt ccggcgtcac cgtgcctgct ccaggcgggc
     2581 cgcgtcggcc ggcggcaggc tggccacttc ggcgtgtccg ctcagttgca gcgcacgtat
     2641 atatgccgcg atcgcccagc ggtcggctgg tggcacgcga gccgcatacg agaacatcac
     2701 cccatagccg ttggtgatga cgtcgacgaa gtgttcggtc ggggccgagc gcaggcgctc
     2761 ggcgtgaagg tcgggcggcc gcggaaatcc acgctgcacg atcatgccgt cgccgtcacc
     2821 gctatggccg tggcaaggcg agcagaagac ggtgaagcgc tcgcggcccc gctcgatcag
     2881 cggccgatcg agtggcggcc gctcggtcag cgccgcacgc catgcgagct cgccgcgggc
     2941 gatcgtgccc ggcacgggag gctggctgac cttgccgtcc gggaacagat cggcggcctc
     3001 gaaggcttcg tacttggcct ggtcgtccat cgactgatcg cagccaccga gcagcacccc
     3061 gaccaacgac agcaggcaag ggaacctgcg gctcatgccg gcacctcctc gaccacgctg
     3121 gcaccgagac cctccagcag ccgcgccgtc gcctcacgat cgaagcacgg atcttcccgg
     3181 cgcaccgaca agcagaagcg atcgaggctg acgcggcggt aggagggcgc atcgaaaagc
     3241 ggatggtgaa gccgcggcag gccgttcagc cacagcatgg cgcccaccac ggccagcacc
     3301 gcgccgagca ccgaggtttc gaacgctggc acggcgaagg ccggccaggc gtcgagcggc
     3361 cggccaccga cgttgatcgg gtagtcgtag gcattgagat accattgcat cagcagcccg
     3421 ccgctgccgc cgaggatgcc accggcaagt gcgatgaacg gcagtctgct ctcctcgaag
     3481 cccagggcgt catcgagccc ctcgaccggg aaaggcgtgt ggccctcgag ctcgcgatag
     3541 ccggccgccc gcatttgacg ggtcgcgcaa agcagagcgt cgggatcggt gaagaccgcg
     3601 agcaggccat agaggcgatc cggctgctcg cgctcactca tgggtcccct cctcgctcag
     3661 cacttcctgg acctcgaaca tcgagatcat cggaagcagg cgcacgaaca ggaagaacag
     3721 aaagaagaac agcccgatgg tgccggcgaa cagcgaccag tcccagaagg atggccagta
     3781 gacctgccag gcggacggca ggaagtcgcg gtagagcgcg gtggtcaaca gcatgaatcg
     3841 ctccgtccac atgccgaagg tcacgagccc ggcgatcacg gcgaggagcc agacccgccg
     3901 ccgcacggcc gggaaccaga gcggctggat cgcgagcacg ttgcatgcga tggtcgccca
     3961 ataggcccag gcatagtcgc cccagagacg atcgagcatc gtctggcgct cgaacggatc
     4021 gcccgagtac caggcgtgga aggtctcgaa aacgtagccg tagctggtca tcaagccggt
     4081 cgcgagaacc agcttggcga ggatgtcgag gtggcggggg gtcaccagat cgtaaaaggc
     4141 gaaggtggca cgcagcgtga tcgagatcag cagcaccacc gcgaaaccct cgaagaccgc
     4201 gccgaggacg aagtagacgg gaaagatcgt gctgtgccag ccgggctcga ggccgacggc
     4261 gaacagcagg cttacaccac tgtgcaccga gaccacgagc ggcacgccga tgccggcaca
     4321 gatccagtag ctcacctgcc agcgcttcca gtgccgcgcc gaacctcgcc agccgagcgc
     4381 cagcaggccg tagagcacct ggggtgcgcg ccttgtcgcg cggtcgcgca tcgagccgag
     4441 atcggggatt aggccaacat accagaaggt caccgagacg acgagatacg tgcccacggc
     4501 gaagacgtcc cactcgagcg ggctcttgaa ctgcggccac acggtcatcg tgtcgggata
     4561 cggtatcaac cagtagaaca gccacggccg accgaggtgg aggatcgggt acatgccggc
     4621 catcagcacc gcgaacacgg tcatcgcctc ggcgaagcga ttgagagagt tgcgccagcc
     4681 ctggttcatc agcagcagca gcgccgagat cagcgtgccg gcatggccca tgccgatcca
     4741 ccacacgtag ttggcgatcg cgagccccca tggcaccggc atctggatcc cccagacgcc
     4801 gaccccgccc cagaacagcc agagcacgga aaccgcccac acgacgacga agacgctgca
     4861 gacggcaaag ccgatgcgcc actgccgggg atgcgggtag tcgagcggaa tcagcgcgac
     4921 ttgccggccg acggcacgat agctcagatg gcgcggcagg atatcgctac gtcgacgtct
     4981 cacatcctgt gcggtcgtgt ccattgctca cgccggctcg gagccgcgcc ggccggacgg
     5041 agccaccttg gcgaggtacg tcgtgcgcgg ccgcacgcca agctcctcga gaagcgcgta
     5101 gttgcgtgga ctggccttgg cgcggcggat cgcgctgtcc gggtcgttga ggtcgccgaa
     5161 gacgatcgca cgcgtcggac aggcaccctg gcaggcggtg acgacctcgc catcgcggat
     5221 cgcgcgttcg tccgtcttgg ccgcgatccg cgcccgactg attcgctgca cgcaataggt
     5281 gcacttctcc atgacgccgc gggcgcgcac ggtgacctcc ggattgcgtt gcggcacgcg
     5341 gccctcgctc tcgccgccgg catagtcgaa gaagttgaaa tggcgcacct tgtaggggca
     5401 gtagctggcg caggtccggg tgccgacgca ccgattgtag accatttgat tgagcccctc
     5461 gggtccgtgc accgttgcat tgaccgggca gccgacctcg cagggagctt gctcgcaatg
     5521 catgcacggc accggctgga agtgggtctc ggggttgccg ggagcgccct cgtaataggt
     5581 gtcgacgcgc agccagtgca tctcccggcc catcgccacc tgctccttgc cgaccaccgg
     5641 cacgttgttc tcggccatgc acgcgacgac gcaggcattg cagccgatgc aagtgtcgag
     5701 gtcgatcacc atgccccagc tttcctcggc atagggccag tccgggtaga gcgagggctc
     5761 ctcgaacggt ggcgccacgg gcgtgccggc gcgcacggcc tcgggcgtgg tctggcggat
     5821 cagatcatga ccggccatgg tgcggtggag ctgcgtcgtg gccagcgcag ccgccgcgcc
     5881 caccgcctgc aaggtgacgt catcgaggcg ccacgggtcg gcctcgcggc gcagccggta
     5941 ggcgtcgtag ccaacgccgt tgcctatccg cccggcgagc tttcggccgt agcccagata
     6001 cagggtgacc gtgcgctcgg cctgaccggg caggatccag accggcgcct cgagcaggcg
     6061 ctcgcccgcc gaaatccgca cccgatcgcc attggcgagg cgctgcgcgg cggcgagcgc
     6121 cggactgatc gcggcgacgt tgtcccaggt gagcttggac aagggtttgg gcagctcctg
     6181 caaccacggg ttgttggcga agcggccgtc ccagatcgac ggatcggggc ggaacaccgc
     6241 ctcgagcgtg cccgcctccg ctgtggcggg tggcggcgcg acctcgattg ggcgcggcgc
     6301 atcggtcacc cgcgcggcgg cggtctcggc ggcgaagccg tcgctcagca gcttggccca
     6361 tcgcgcctcg aagccttcct cgccgagcat atcgcgccag gttgtccgca ccgcgtcata
     6421 gggtgtgacg tcaggatcgc ccgccaacgt tgccagcagt tcggcctcgg tgtggctcgc
     6481 gtggaggggc cggatcgtcg gttgaaggat ggtggcggtg ccgtcgaccg ctcgggcgtc
     6541 gcgccagccc tcgaacgcgt gacacatcgg gatgtgccag cggctcgcgg ccgcggtctc
     6601 atcctgatac tggccgagat gcacggtgag cggcacttgc cgcaggagcg ccgcaagatc
     6661 caggtcgtcg ggtgcggtgt agagcgggtt tgtatcgagc atcagcaggg tgtcgacgga
     6721 cccgccccgg agcgcctcgg ccagatcctc gaggtcgccg accccgtcgg gttgccaggc
     6781 cgcaggctcg gtcagctcga tggtatgatg cagcgcaccg aggcgctcgt tgatccggta
     6841 gccgagcgcc tgcagccgcg gcggcaggtg gtggccaagc gccaccagtg cggccttgcc
     6901 ggcctgctcg agttccgcgg atgccgcgag cagccaggac cgcagcccga gcggcagatc
     6961 gaccgcctcg gcgggtccga tgcccaactg ccgggcgagc gccacgacca cggcttccac
     7021 cagcgccgaa tccgccggcc agcgctcggt ggccttggcg ccggtcaggg tcggggtgct
     7081 ctcgaccgcc agcaaccgca tggcacgccc ctcctcgagc gcggcccgcc gggcatctga
     7141 aaacccccgg gcgttggcga tctgcgccgg gccgggcccg agtggatcgg cttccagcgc
     7201 gagcactgcc cgcgcccgct cgagccgata gcgctgatcc aatgggcggc cgaaggcgag
     7261 gaggctcgct gcctgacgcc tttggtcgcc gaccggttcg tggcgaaact gccgcagcgc
     7321 cggaaaacgg tcctgcagcc ggcgcaattg gcgcaccgtg gtcggcgagg tgatcgtgcc
     7381 gctgaggatt gccaggcctt ctccgccgcg acgctccagc tcctgcgcct tgaccaggag
     7441 atcgcgctgc gcctctggcc acggccggac ttcgcccaga taggcggtgg ttcgggaacg
     7501 gtctgggtcg tagaggtcga gcaccgcggc ctgcgcgaac aggtcggtgc cgccgcgggt
     7561 cagcggatgc tccgggttgc cttccacctt gatcgggcga ccgtccacgg tctcgaccag
     7621 gagcggaatg gcatagccat tcagcaggct cgcggctgca tagaggcgcg gctctcccgc
     7681 tgccccgaat tcgggcatgc cgacatgggg caatgcccgc tcctctggct ggaccggatc
     7741 gcagccgccg aggccggcca gcgccagcga cgccgccatg acacgcagca cctcgcgccg
     7801 gctggcttgg ccgggcagcg ggccgaggtc tggaagctcg cgctccagcg cctcttcgag
     7861 cctcgccggc tcgtcgaggg cgcgccagag gcgccggccc ttctcctggt cgagttgccg
     7921 cgtcaggggt cggtggatcg cgaggtgcct attcatggtc agcggtgaca gatgtagcag
     7981 ctgtcgaggt ggcggggctg gatgtcgagc tccgccatgc gccttcgacc aaaggcggcc
     8041 cggtccggcg gcggctgcca gtcgaggttg aaaacctgct ccggcggtcg cagatgcggc
     8101 gccggatcgc ggtgacagtc gagacagaag cgcatgtaga agggccgggc ctgatagatg
     8161 cgggtcattc gatcgacggg cccgtggcac tcggcgcagc cgacacctcg ggcgacgtgg
     8221 atcgaatggt tgaagtagac gtagtccggc aggtcgtgga tgcggttcca gcgcagcggc
     8281 cggtcgtcgg tgaagctctg gcgcaccggg gccagcgctg ccgcgttgct ccacagctgc
     8341 gagtggcagg tcatgcaggt gtggctcggc gggtagcccg cgctggcggc ggtctcgacc
     8401 tggtcgtggc aatagcggca gtcgatgccg agctcgcccg tatggtgctt atggctgaaa
     8461 ggcaccggct gctccatcgc cacccctacg ccggtgaggt agtccgatcg aacgagacca
     8521 ctcgccagca gcagcccgcc ggcgacgagc cccgatatga tgatgagccc caacctgaga
     8581 taggaatccg ccgcccggga aaaaacttga gccatcgacc accgagccct cccgtgtaga
     8641 cacacaacgg ctcgcccctc ggcaagttcc tctctcgcgc cttgtcgatc agccggcgcg
     8701 gcgggtgacg caggcgcggg gagcgccgaa agcaggccga gagcacggcc gggacctttc
     8761 cttcgccgac gcgctcaatg cctgcgccac agtagcgagg aaagccggaa gggcgtcagc
     8821 ggatcgagga tgcgggtgcc gggcatgaca tgcgtcggtc cccggtctcg aatacccagc
     8881 ccctcgagcc tgcgaggacc gtcattcagt cgttcgatcg cggcgatgat ggagcctgtg
     8941 gatcgggctg cttcggcgaa cgccccggca gtcttgccga ggtgctccgc aatcacgcgg
     9001 ttgcggacgg cacgaatgcc ggcggccgtg cgctgctcgc cggcctcgag ggccatgtcg
     9061 cattcggcat cgagccccat cgagcggcgg ttgaggttgg acgagccgat cttcaagaac
     9121 tggtcgtcga cgacgagcag cttcgagtga acgagaattt cgacctggct ggagccacga
     9181 acgacggggc agtatgcgcg aaagcggccg tgcctgtcgg cgcggcggag gcgcctgacc
     9241 agtcggtcgc ggttgttccc catgacccat ttctcgatga cgccgcgtgc agcccggctg
     9301 acgaccacga cgatctcggg cccgccttct cgcgccagat gtctcgccag aacgtctgca
     9361 atccggggtt cggcgaagta ctgcgtttcg atgtatatcc gactcttcgc cgcgacgaga
     9421 gcgtcgagaa cgagcgtctc cacctcgcgg acctcgggcg caccgttata ggccggcgcc
     9481 gttcgcgcca cagccatggg aacattctcg aaatcgggct ccacgtcaga cggccaaggg
     9541 tcgagcgaac tcgtcgagga tgtgatggtc tcgccggtca ccactcgcca gcgctcccga
     9601 gcgatccgac agacagcttg cgcggccgca ccatcgatca tccactgcag gtcatgaacc
     9661 ggtcggtagg cgccgccgtc ggggctggtg cggccgccgt cgttcggggc gtggcgggtc
     9721 gtgtcccagc gttcgaccgt caggtcgatc ccgccgacga aggcgatgga atcgacgcag
     9781 acgatcttct gatgctgcgc cgcgtagatc gggtgcaggc tgtccagcgc gacccggatg
     9841 cggggatgct catgccagga ggcgccgaac agcaagggag cggtcgcgcc aggcgaatgc
     9901 acggtcgcca ggttccagac cagcacgcgc acctcgaggg tcggccgctc ctccaccaac
     9961 gcgcgcagga aattgccgag ttgttccgac tcccgcgagt ccgggtggag ggcgatgccg
    10021 gcgtcgaaat cccacccgag gatcagcacc gagcgttcgg ccttcctgag gacgctgtgc
    10081 agggcagcaa agtaccgggc agcgtccacg ataacagcgg cacgccgcgc ggccgcgatc
    10141 gtgccgcagt tgcgtccgac acggaggatg ggtcccgcgt gtggacggat cgaacagccg
    10201 ccaccgcgtt gcttcatgtg agttctcgcc gctttcagca cgccggccga cgggagccgg
    10261 cgagccggct cgctttcggg caacgtcatg acctagacct gcttgaccgc ggtttcccgc
    10321 ggccaatggc ggagcttgcg gcaggcctcg acaaagggct gggcgtcgct gctcgagtcg
    10381 agcgcgatgc acgcttcgtc cagcgcctcg aggatgccgg ccttccgcag cagcggctcg
    10441 gcggcgggcg aataggcgat gaacttcagg tgggcgaagg catcggctac gaagtcgcgg
    10501 gccgtcgcct cgtcgacgag ctgggcgcag ccggcctcgg acgggaggat cgccactgcg
    10561 tcgaagagga ccgaggggcc gccgtcgatc ttctcgtccg cctcgagcca ggttccgtcg
    10621 ctggcttcga cgccgcccac catgggggcg acgagcttca gcatggcgcc ttccttctcc
    10681 agggcggtct tgagcgcggc gaggatctcc gcatcgacgc catcggtgac gagcgcgccg
    10741 accttgcgcc cggcgaacga ccgcgggccg ttgagcagga tgctgagggc tggtgacctt
    10801 gccagatcgg tgcgcggctc gacggcgggt ggggcggcgt tgggcaggct cttcaggcgc
    10861 agacccgcgg ccaccttcgt gcccagatct tcgtcgacgg tgagcaggtg ggccaccatg
    10921 cgctcgcgga tcgccacggt ctcgaccttc gagagctcga aggtgaacgc cgacacgata
    10981 tggttttgct ccacctcggt ctggctgcga tagaactggc gtgcctggct gtagtggtcg
    11041 gcgaagcttt ccgggcgcat ccgcagcttc tggccgctca cctcggccgg gtaggtgcga
    11101 aaacccgttc tcggcgcctc gcgcggcccg ccgaacgagt tcggctgata cagggcgcgg
    11161 cccttcggca cctgcatgcg catgtggccg tcctgctgga aattggcgaa cgggcagccg
    11221 cgcggactgt tgacggggat cttgtggaag ttcgggctgc cgagccgctt gagctgggtg
    11281 tcgagatagg agaagttgcg accctgcagc aaaggatcgt ccgtgaagtc gatgccgggc
    11341 acgacgttct gcgtgcagaa ggcgacctgc tcggtctcgg cgaagaagtt gtcgacgacc
    11401 cggtcgagca ccatccggcc gacccgccgg atcggcagca cctcctcggg gatgatcttg
    11461 gtcgggtcga ggacatcgaa gtcgaattgc tccgcgaagg cctcgtcgaa gagctgcagc
    11521 cccatctccc attccggata gtcgcccatc tggatcgcgt cccagagatc gcgacgatgg
    11581 tagtccggat ctgcgccgtt gatcttcagc gcctcgttcc agaccaccga ctgcatgccg
    11641 gatttgggct tccagtggaa cttgacgaag gtcgacttgc cctcggcgtc gaccagccgg
    11701 aaggtgtgga cgccgaagcc ctccatgaag cggagcgagc gcgggatcgt ccggtccgac
    11761 atgatccaga gcgccatgtt gatgctctcg ggcgtcagcg agatgaagtc ccagaacgtg
    11821 tcatgcgccg tctgcgcctg cgggaagccg cgatcgggct cctgcttcgc cgcgtggacg
    11881 aggtccggga acttgagcgc gtcctggatg aagaagaccg ggatgttgtt gccgacgagg
    11941 tcccagttgc cctccctcgt gtagaacttg acggcgaagc cgcgcacgtc gcgggcgatg
    12001 tcggccgagc ccttgttgcc cgccacggtc gagaagcggg cgaaaacctc ggtcttctcg
    12061 cccttgcgct ggaagaggtc cgcgcgagtc acgtcggcga gcgactcgta ggtttcgaaa
    12121 tagccgtggg cacccatgcc gcgcgcgtgc acgacccgct ccgggatgcg ctcgtggtcg
    12181 aaatggaaga gcttctcgcg aaaatggaaa tcctccatcg ccagggggcc gcgcggcccg
    12241 atccgcagcg agttctggtc gtccgaaacc ggcaccccct ggttggtggt gaggacctcg
    12301 ccatcgccgc cagctatctg gtggtactcg ccaccgtcgc cgcgctgcag cttctggtcc
    12361 gcgacctcga tcgcgtcacg cttctcggac atggatttcc tcctcgacag ggttgaagga
    12421 atggagtgaa ttcaggacgt tcgtgccgcc tgctccgcca tgagcagccg gtccggcgtc
    12481 agcggcagct ccctgatccg caaccctgtg gcgtgccaca cggcgttggc gatggcgggt
    12541 gcgacgccgc agagcgcgat ctcggcgatg cccttgacgc cgagggggtt gaagatccga
    12601 tcctcgctct cgacgaagac ggcctccagc tcctccacat cggcgtggac aggcaccagg
    12661 tactcggcga gattggcgtt catgacccgg cccaggcggt cgtcccattc gccggcttcc
    12721 atgagcgcca tgccgatgcc ctggaccatg ccgccgatcg cctggctgcg ggcggtgcgg
    12781 gggttgacga tgacaccgcc gtcataggcg ccgaccagcc gtggcacgcg gacggtgccg
    12841 aaatccggat cgaccctcac ttcggcaaag acggcgccga aagcgtagct ggaatgcgtc
    12901 ccggcttccg cgcccggctc ggccgcgcca tcgacctcga agctgtcacg gcgaaggcgc
    12961 atgagcgtct ccaccggatg ctcgccgtcc tcggcgccgt cgagcctggc gatgtcgtcg
    13021 cgaagcttct tgcagccggc cgcgacggcg ttgccgacgc tcgccatcgt catcgagccg
    13081 ccatggacgg gcgccatggg caggctgctg tcgccgagct cgaaggtcac ccgctcgacg
    13141 ggcaggtcca gacggtccgc cgcgacctgg gtcatcgagg tataagtgcc gggacccatg
    13201 tcgctggccg ccgtccgcac gaccgccgtc ccattggcga agagcctgat gcgcaccgag
    13261 gcgggtgcct gcatcgcggg ccagaccgcg gtcgccatgc cgtagccaac gagcatgttg
    13321 ccatcgtgca tggcccggcg ccgcggatcg cgcttctccc agccgaagcg aagggccgcg
    13381 gtctcgtagc acgatcgcag cgccttgctc gagaagggga ggtccttctt cagatcacgc
    13441 tcggcgtagt tcttgagccg gaactccagc ggatccatgt ccatggcgtc cgccatctcg
    13501 tcgatcgcca tttccagcgc gaagacgcca gtgacggcac ccgggccgcg catcggcgtc
    13561 ggcgtattgg tgtcgagctc gaccagccgg tagacggtcc tcgcgctcat cgtatcgtag
    13621 agcatgccga ctggatcgag gacttcctcg ccgtaatcct cataggccga cgtgaggccg
    13681 taagccgtct ggatgacgga gcgcagcctg ccgtcctcgt ccgcggccag cttgacgtcc
    13741 tgcagcgtgt aggggcggta gccgaccgcg gtgtagagtt cccgccgcgt gagctcgagg
    13801 cgaaccggcc ggcgggcgat cctcgctgcc atggcggcca tggtgacgtg cggccagcag
    13861 cgcagcgccg aaccgaacgc gccgccgacg aaaggcgaga tgacccggac ctcgtcctcg
    13921 gccagcccga aattgcgcgt gatctgatcg cggacgttgt cgacccattg cgacttgtcc
    13981 cagagcgtca gcttgccttc gtcgtcccag gcggcaatgg tcgcatgcgg ctccatcgca
    14041 ttgtggaaga gccgcggctg gagatagctg ccctcgatgc gccggggggc cgaggcgaaa
    14101 gcgccctcgg cgtcgccccg ctcccattcg gccgggtagc tctctcgagt cccgtcgctg
    14161 ggcgggcgag ccctggcgcg gtcgaagacg agccctgcac cgtcactcgg gacgcaggtg
    14221 aaggagacaa gggccgcggc cgcgcgcgcc gtttcctgtg attcggcaat cacgagagcg
    14281 atgggctgac cggcgaagag gatctcgtcg gagtgcaaag gcctgagctg ctcgccctcg
    14341 cctggatcga ccggagccgc atcgaccggc ttccaaggca gccgtggggc attcagatgg
    14401 gtcacgaccg cgatgacacc cggcacgctc tcggccgcgt cggtcgcgat cgatttgatc
    14461 cagccccgag ggatcgtgcc gtggacgagc gccgcatagg ccatgttctc cgggtggaac
    14521 tcggcggcat aggtggctgt accggtcacc ttggccggac cgtcgacgcg agctaccggc
    14581 ttgccgatgg cgcccatcag agcctctccc tcaactcggt cagggcgcgg gcgatcgtgc
    14641 gcggcagcag cgccagcttg tacgcgttgt ccccatgggc cacggcgttc tgcacggaaa
    14701 gagcggcggc ctccgcgaag gcttcggccg tggcttggcg cccctgcagg gcagtctcga
    14761 cctgccccag ccgccaaggc ttggtggcga cgccaccgac ggccacacgc gcgtccgcga
    14821 tcctgccgcc gtggtccagc tcgagggcga cggccgccga gcagatcgcc cattcgaact
    14881 cgccccggtc gcggaccttg agatagtgcg aatggcgggc gagcggtgtg cgggtgagcc
    14941 ggtagccttc gatgacctcg tccggggcta gattatgctc cagatgcggg gtgtcgcccg
    15001 gcagtcggtg aagctccgtg aagggcagcc ttcgctcgcc ccgcgcaccg acgaccagca
    15061 ccgtcgcatc gagggccgcc atggcgacgg cgaagtccga cgggtgagtt gcgatgcagt
    15121 gctcgctgcc gcccaggatc gcgttgatgc gattgcgccc ttcgagcgca ccgcaccccg
    15181 acccgggctc ccgcttgttg cagggtgtgg actggtcgcg gaagtacagg catcgggtct
    15241 tctgcaacgg attgccgccg accgtcgcga gattgcgcac ctgcggcgag gcggtgacga
    15301 tcaaggcctc gacgatggcg ggaaaggcgt cgcgcaccgg ggcactctcg atgacctggt
    15361 tcagacgcac gagcgcaccg agacggatct cgtggtcgtc gacctcgatc gcacgcaacc
    15421 cctcgacgcc gttgatgtcg acgaccaggg gcggcggcag cacgtgctcc tccagacgct
    15481 ggagcagatc ggtgccgcca gcgatgacct cggcagcgcg atgcgagccg atcacctccg
    15541 tcgcgctctc gagcgacgcg gcacgggcgt aggcaagccc gctcatggcc tcgtccccac
    15601 ggcggtttcg aagatcgcct cgacgatgtt cacgtaggcg ccgcagcggc agatattgcc
    15661 gctcatgtat tgtctgatct cggctctcga accggcatgg ccttcctcga tgcagcccac
    15721 gcccgacatg atctgtccgg gcgtgcagta gccgcactgg aagccgtcat ggtcgaggaa
    15781 ggcccgttgc agcgggtgaa gcagaccatc gcctccgcgc aggccctcga tcgtggtgat
    15841 gtggcgcccc tgggccatga ccgcgagcac cagacaggca ttgacccgct cgccgtccat
    15901 gaggacggtg cacgcgccac actgacccag ggcgcaaccc ttcttggtcc cgttcaggtg
    15961 aaggcgatcg cgcagcgcat cgagcagggt ggtgcgcgcc tgaacgtcga gggcgtgctc
    16021 cctgccattg acctcgaggg tcacgcgcat cgtcgagcgg gcctggacag cgccaccctc
    16081 ggcaccagtc gaagcgttct ccatcgtccg tggcctcctc ggcggaagta cgaatacacg
    16141 gggacgacaa gctctcgctt ggcctgcctg cgacccgcgc cgtcctcgga cgctcaaccc
    16201 gtcatgtggt cggcggttcc ggcagagctc gggcaggcca tccctccgag ggcgagctgg
    16261 ctctgtgcca cgtttgagga agcttgctcg cgaaccctgt ggcgagttcc gcaaggctgc
    16321 gttggcaccg agcctcagcg gcgctttttt tcctcggcca ggctgcgccg gagcgcatcg
    16381 aagatggaaa ccacgttatc gccgctggcc gccaccgggg cggcggtccg ctggcgcttc
    16441 ggcgcgggct tccgcttgcg ctgcttcgcg gcgacgagcc cggccagtcg accttcgacc
    16501 ggatcggtga cgagcgtcgg ggcccaggga cgggtgcggg cctcgatcag ctcgcccagg
    16561 agccccatca gcttctcgtc gggcccctgc tcctcgacct tcgcgaaggc cgcctcggcc
    16621 gagcgcacct cgtcgccgta gcgcagggtc caggcgacga tgcccctctc gcgcggctcc
    16681 agcatcaggg cgcgctcccg ccgatacagc accactcgcg agatggcgac agtgccggtc
    16741 cgctccatgg cctcgcggat gacggcgaac gcctcctcgc ccaccctgtc gtcggggaag
    16801 aggtaatgcg gcgtgtcgag ccagatccag ccgatcgaat cgcgctcgac gaagcagtcg
    16861 atgtcgatcg ttcgcgtgct ctcgagcgcc acgctctcca gctcctcgtc ctcgaggatc
    16921 acgaggcgat cttcggccac ctcgtaggcc ttcgccaagg cgtcgtcggt gacgggcgtg
    16981 ccggtttcgg cgtcgacata gcgtgacgtg atcgtgttgc cggtctcccg gttgagcgtg
    17041 cggaagcgga tcttttcccg gtccgagctg gcgggcacca tggccacgcg acagctcacg
    17101 agagagagct tcaaatatcc tttccagaac gttctcggcg ccatcgcacc acctttcgat
    17161 tgccgtccca accagggcca gcggcgaaag gttcccttga agatcccgag tggccgcact
    17221 tccggaatcg ccaccacgtc gcggtctctt tggaaggacc cgaaagagcg gccacggccg
    17281 cgtgtgggcc atttgcggga tcgacgacgt gagagcttgg cggatggggt gggattcgaa
    17341 cccacggtgg ggatgaaccc acgccggttt tcaagaccgg tgctttcaac cgctcagcca
    17401 cccatccgat gactcgagat tagtgcatcg ggcgggcgaa cggcaatcgt tgcgcggcgg
    17461 gcaatggggc cgctgcgacg gtcgtcggga ttgacggtct cgtggcgcgg gtctatggcg
    17521 atcgatcatc tggaacgacg ttccgccaaa tggaacacga cggcaactcg acagggaagg
    17581 ccaagcattg ccatgagcca ggttcgtgat cgtcttttcg gcatcgtgcc accgacgacc
    17641 accgcgttcg atgcggcggg cgagatcgac gaggccggga ttcgcgccca ggtgcgcttt
    17701 ctgctgggcg ccggggtgca tgggctggcg gctggcggca gcaccggcga ggggcatacg
    17761 ctcgaccacg aggagttcgt gcgcctcatg gagacgaccg tcgacgaggt ggcggggcgc
    17821 gtgccggtcg ttgccggcat catcgtcgac agcacgcgcg acgccatccg ccgcgccagg
    17881 tcgatcgcgc acctggatgt ggtggccctg caggtcacgc cggtgcacta cctgttcaag
    17941 ccgagcgacg acgccatggt ggagcatttc cgccggctgg cgggcgagac cggcaagccg
    18001 atcctgatct acaatgtcgt gccctgggtc tatctctcgc cgcagctcct ctatcgcatc
    18061 ttccgcgagg tgccgggcgt ggtcggggtc aagcagagtg ccggggacct caagcgcgtc
    18121 gccgacctcc tgttcatcac cgagcccgag gatctcgtct tcacggccgt cgacgccctc
    18181 ctctacccgg ccttcatgct gggggcgcac ggcaccatcg ccgccctgcc gaccgcggca
    18241 ccgcatgcct gcgtggcgct ctgggacgcc tgccaggcgg gagaccatgc ccgggcgaag
    18301 gagctgcatc tgcagctcct gcgggtctgg aacgcgatcg aggcggacaa cctgccggcc
    18361 tgcaccaagt atgcgctgac cctgcagggc tgtgccgccg gcctgccacg gccgccgatg
    18421 cacgcgccga gcgagacgca gaagaagaac atccgcgaag ccttgcaggc gatgggggta
    18481 tcgctacaga ctgccgcctg aagctgcacg acaaaacgcc cggggaggca acaatgaacg
    18541 aaagagcagg aatccgtctg gcggcagcgg ggctggtcgc cgccgtcacc ctcgccacga
    18601 cctcggcggc gctggccgcc tggccggagc ggccggtcac gttgatcgtg ccctggtcgg
    18661 ccggcggcgg caccgatgcc accgggcgga tcgtggcatc gatgctggag gagcggctcg
    18721 gccagccctt caacgtcgtc aaccgcaccg gcggcggcgg cgtggtcggc cataccgaca
    18781 tcgccaacgc ggcgcccgac ggctacacgc tcggcgtcat caccaccgag ctctcgatgt
    18841 tccactggat cggcacctcg ccgctgacct tcgaggacta cagcctcttc gggctctaca
    18901 acaccgatcc gacggcgatc cacgtgcggg ccgacttcga ggcggccgat ctcggcgcgc
    18961 tgctcgagat ggtcgacgct gcgcccgggc agcacaaggc ttccggcgcc aaccagggcg
    19021 gtgcggcgca tctcgggctc gccggcctgc tgcaggcctc gggccgcgca ccggacgcca
    19081 ttccctggat cccggccgag ggggcggcac cctcgctgca gctcctggtc tcggacgcca
    19141 tcgacgtcgt ctccaccacc atgcccgagg tgcgctcgct ggtcgatgcg ggcgaggtca
    19201 ggacgctggc cgtcatggcg agcgagcgtg acccggcctt cccggacatc ccgaccgtcg
    19261 ccgaggccat cgacgtcgac tggacgaccg gcgcctggcg cggcatcgcc gggcccgccg
    19321 gcatgccgga ggaggtcgcg gaggtgctgg tcccggcgct cgaggagatc gtcgccagcg
    19381 ccgagttcaa ggagttcatg gccggccgcg gcttcggcgt cacctgggcg gcacccgacg
    19441 acttcgcggc ctatctcgcc acggccgacg cgaatttcgg tgcggcgatg caggcggtgg
    19501 gtctcgccaa ataggtcatg cggcttgccg atggcctgtc gggtggcgtc gtcgtcgccg
    19561 gcgccgccct cctcctctgg acggcgtccg gcctccccga catgcccggc cagcgtttcg
    19621 gcccggcgac ctttccgacc ctgatcggca tcgccatggc tgccgccggc ctggtcgtgc
    19681 tctggcgcgg ccggcccgag cgaccgctct tctcgatcgg cacgctcggc catgaccgcc
    19741 gggtgcaggt ggccgtgctc tggctgctcg gtggtctggt ggcgatgctc gtcctctggc
    19801 agccggtggg ctttccgctg ctcgccgcgg tctacagtgg cgggttgatg ctgatcctcg
    19861 gcgtgcgccc ccttccggca gtcttctggg cttgcgccgt ggccgtcgtt gtccatctcg
    19921 tcttcacccg cctgctcctc gtgccgctgc cggccgggcc gctcggcttc ctgctctagg
    19981 catcgcccat ggccgacctg ctcgccgcct gggcactggt gctcgatccg gaggtgctgc
    20041 tcgtcatcct ggcggcggcg ttcttcgggc tcttcgtcgg cgcagtgccc ggcctcacgg
    20101 cgaccatggc gacggcgctc ctcgtgccgg tcaccttctt catggcgccg ctgcccgcgg
    20161 tcgccgccat cgtcgcctgc accgccatgg cgatcaccgc cggcgacata ccgggtgcgc
    20221 tcctgcgcat tcccggcacc ccggcctcgg ccgcctacac ggacgaggcc tatgcgctga
    20281 cccgcgccgg ccagccggag ctggcactcg gcgccggcct cgtctgcgcc gccctcggtg
    20341 gcctgttcgg tgcgctggtg ctggtcgtcg ccgccccgct gctcgcccgc atcgccctcg
    20401 gcttctccag cttcgagtat ttctggctcg cggccctcgg cctcacctcg gctgccttcg
    20461 tcagtgccgc ggaccgggcg aagggcgtgg tctcgctcac gctcggcctc ttcatcgcga
    20521 cgatcggact cgacatcacc tcgggcctgc cgcgcttcac gtttggctcg gtcgagctca
    20581 tgggcggggt cagcttcatt cccgcgatga tcggcatgtt cgcggtctcc gaggtgctgc
    20641 gcaacatcac cgatggcacg tccctgggca cggtgccgaa ggtcaatgtc ggcaacccgt
    20701 tcagggggct cggggccgtg ctctggcgct atcggctgaa cgtgctgcgc ggcagtgcgc
    20761 tcggcacgac ggtgggtgcc ctgcccggcg ccggcggcga catcgccgcc tggatcgcct
    20821 acgccaacgc caagcgcttc tcgaagacgc ccgagaagtt cggccagggc catgtcgagg
    20881 gcatcgtcga ggcgggctcg gccaacaata gcgggctcag ctcggcctgg gtcccggcca
    20941 tggtcttcgg catcccgggc gacgccatca cggcgatcgc catcggcgtg ctcttcatga
    21001 aggggctcaa tcccggcccc aggatcttcg tcgagcagcc ggcgatgctc tatgccatct
    21061 tcatcacctt cttcctcgcc aacctgctgc tcattccctt gggctggctg gcgatccgtg
    21121 ctgcccggcc gatcctcgcc gtgccgcgca acctgctcct gccgctcatc ctgatcttct
    21181 gcgtggtcgg cgccttcgcc atcaacaaca ccgtcttcgg catcgccgtg atgctggtcc
    21241 tcggcgtcgt cgcctggctc atggaatcga gcggctatcc gatcgccccg gtgatcctcg
    21301 ggatcgtcat cggccccctg ctcgagcaga acttcatgat ctcgatgatg atcggcgacg
    21361 gtgatctcct ggcctttttc gcgcgcccga tcgccgccgt gctaggtgtc attacggtcg
    21421 cggtctggtt ggcccccctg gctctccggc tcgcccgtgg atggcggccg ggcccacatc
    21481 cttgaagccg atacccttga aggagctccg cttggatacg ccatcgacgc ttgagaaccc
    21541 ggccatcgat gggcttcggg ccctcttcgg cgagcgctgc tcgacccggc ccgccgagcg
    21601 cgagcagcac ggtcgcggcg aatcctacca tccctgccgg gcgcccgacg ccgtctgctg
    21661 gccgggctcg acggacgagg tggtcgccgc ggtggagatc tgccggcggc acaggtttcc
    21721 cattgtgcct ttcggcgtcg gcacgtcgct cgaggggcat gtggccgcac tggaaggcgg
    21781 tgtggccctc gacctttcgc gcatgaaccg catcctgatg gtgcggccgg aggacatgga
    21841 cgtcacggtc gaggccggcg tgacccgcaa gcagctcgac caggagctgc gcgacagcgg
    21901 gctcttcttt ccgatcgacc cgggtgcgga tgccacgcta ggcggcatgg cggcgacccg
    21961 cgcctcgggc accaatgcgg tgcgctacgg cacgatgcgc gagaatgtcc tggccctgac
    22021 cgtggtcctg gccgacggcc gcgtcgtcaa gaccgccaac cgggcgagga agtcgtcggc
    22081 cggctacgat ctcacccggc tgttcgtcgg ctcggagggc acgctcggcg tcatcaccga
    22141 ggtcacgctc cggctccacg ggcgacccga ggcggtggct tcggcggtct gctcgttccc
    22201 gtccgtcgac caagcggtcg ccaccgccat ggagaccgtc caggccggca ttcccgtcgc
    22261 ccggatggag ctcttggacg gcaatgtcat ggaggcggtc aaccgcttct cgaagctcga
    22321 ctacccggcg acggcgaccc tcttcctcga gtttcacggc agcgccgcca gcgtcgagga
    22381 gcaggcggcc atggtcggcg agctggcagc cggcaatgac ggctcggcct tccgctgggc
    22441 gcttcgcccc gaggagcgca atgccctctg gcaggcgcgc cacgacctcc actacgcgac
    22501 gctggcgatg cgaccgggtg ccaaggtctg gggcaccgat gtctgcgtac cgatctcggc
    22561 gctcggcgaa tgcatccggg aagcccgccg cgccgccgcc gaggcgccct tcttcgtcac
    22621 tacgctgggt catgtgggcg acggcaattt ccacatgggc tatctgatcg acatggccga
    22681 cccggcggag ctgcaggcgg ccgaacggct caacgagcgc ctggtccgcc atgccatcag
    22741 cctcggcggc acctgcaccg gcgagcacgg cgtcggctat ggcaaggcga agttcatgac
    22801 gctcgagcat ggcgacgcgg cggtctcgct gatgcgccag atcaagcaca tgctcgaccc
    22861 ggagaacctc ttcaaccccg gcaagatcct gccgccggcc taggggcggg aggttaggga
    22921 aatgtccagg tcagcgcgaa atcctggtcg agggcctgca gctcgaacgt catcgcttcg
    22981 gccgtcgccc agtcatggcc gtccggcaga acgaccatga ccctggcccg ggcggcgtgg
    23041 agggcaccac cgagggcggg ctcgagcgcc acatcgtgtt ccaggaaagt gccgggcaga
    23101 ttggagcccg tgctgtcccg aaaggtgacg atcagggcgt cggagcgcaa ggcttcgggc
    23161 gtttcggcga tcacgaggag gtcgaagacg aggccggtgg tcgggtcgag gtagagctcg
    23221 gcgaagcggc ggtcggcatc gccctccgcc agga
//
LOCUS       DAOTFI010000040        22618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2702_length_22618_cov_7.407526, whole genome shotgun sequence.
ACCESSION   DAOTFI010000040 DAOTFI010000000
VERSION     DAOTFI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22618
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2702_length_22618_cov_7.407526"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4091
                     /locus_tag="P5558_05800"
     CDS             <1..4091
                     /locus_tag="P5558_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY23854.1"
                     /translation="VRLRVSGFKSFCDATELDIGHGLTGIVGPNGCGKSNIVEALRWV
                     MGESSARGLRGGEMDDVIFGGSAMRAPFDLAEVALGLKQAPRAAGDAAIHVLPSSEDL
                     EISRRIGRGTGSLFRLNGREVRARDIQLLFADAGAGAKSPAIVGQGQVGFIVEAKPEE
                     RRRLLEEAAGIGGMHSRRREAELKLAATESNLERITDQLGLLAERRTALEKQAREAER
                     YKKLQGQIRALDAWLVLARITAGRTALAAAKEALALARGRLGEERTRAAAAEAAHDEV
                     VRRLPDLRDRASVALALRARLAERVEQVERIAAQEQAERARLQADRDDTQGRIAALGE
                     AIDAGNARHDTILATDRKLASTIETLTAERAAIGPSSDAAAQRLQALGQEVQEASARR
                     AELERRLEGARARHEERHRRLRSVEAELLRLADVADDALVEAALRKRDEAAGLLAVAD
                     AELAAAQAERDAAAAPLDRLKAEVEAASEAVRQAAARVASLDAEIGRRRTALAGLAER
                     RQWHERALGQATRAAAEADEALAALAPEQPDEDGELAAKLGALEARAAALETELADAV
                     PEVARAREALATAECGLAAHDAERLKLAAEAEALALIDAPDAEAPVLDLLRVTAGCET
                     ALAAALGDDLMAGLDEAAATHWQEVPGEIAAPDLPPGAVPLAERVEAPAALMRRLRQV
                     GLVADRATALRLLPALAQGQRLVTADGGLWRWDGFVRGAEADDTMRRRLAQRQRRQML
                     QARLAGLDGDRAALARVVEVATGELASAQDGEVALAKERAELATAIDRAAQARETAAV
                     ARRHRQAERQRLTETQARSRAERAEHEKALAELEAAGEAHAIEAVSGEAATARAALAE
                     AEEQAKTLVAALGAATRAHQAAASRAGEAEASQRRAAQDALAARLLHERRQGEAELRK
                     SHRADAVTGLDTEARQLRGELEGLDEEIEAGTTGLAAATATSERLAASHAEAGIEAER
                     LRTRRAAVDAEERSAQDQRRALADELAHLIADLDRRGADLVATRQRLQTVEQELARAV
                     AASPGDDPGLALRRELEAAECQASEAASALAAAEAELQACEAQRRTADADLARAHEAS
                     ALALADVERLGEAQRELERETTRRLGQPIEAALAAAEESGVERSLEGEPPDVLEARLE
                     KLKTSRERLGAVNLRAALECEELAAEIAGLEHEAGELRQAVERLQRAISTLNREARGR
                     LEAVFDDVDRHFRQLFQRLFGGGKAHLRLTNMDDPLNAGLELEAMPPGKKLQNIRLLS
                     GGEKSLTALALVFAFFLTQPSPLCVLDEVDAALDDANVERFADLLEEMAKETKTRFLV
                     VTHHPLTMARMDRLFGVTMAERGVSKLVSVAFDEAERLRATA"
     gene            4247..4579
                     /locus_tag="P5558_05805"
     CDS             4247..4579
                     /locus_tag="P5558_05805"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRY23855.1"
                     /translation="MPEDGKPPGFSDFDARLAATRQARSDKRTSDDAAERPHFDWGRG
                     MQAGIEVVAGVAGGSLLGWALDAWLGTGPFLFIGGFMLGAAAGMLNAFRNLRRWMGGD
                     GNGPAQGQ"
     gene            4630..5352
                     /locus_tag="P5558_05810"
     CDS             4630..5352
                     /locus_tag="P5558_05810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRY23856.1"
                     /translation="MAGPLEQFEIKPIVRILIEGVDLSFTNSALWMVIAVAAVYYFVV
                     LGTRQHALVPGRLQSLVELSYEFVAGLVRENAGKEGMRFFPLIFTLFMFIFMGNMLGM
                     IPGSFTFTSHIIVTFFMALCVWVGVTVYGFYLHGARFLTFFAPSGAPVYMLPLLVPIE
                     ILSYCVRPISLSVRLFVNMMAGHTMMKVFAGFIIALGVFGVAPLAIAVALTGFEFAVA
                     FLQAYVFTVLTCLYLHDAIHMH"
     gene            5426..5650
                     /locus_tag="P5558_05815"
     CDS             5426..5650
                     /locus_tag="P5558_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015087984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C family protein"
                     /protein_id="HRY23857.1"
                     /translation="MELEAAKMIGAGLATIALFGVGIGIGNVFSTLISTVGRNPSVQQ
                     RVFPFAIIGFALVEAVALFALLIAFLILFT"
     gene            5727..6359
                     /locus_tag="P5558_05820"
     CDS             5727..6359
                     /locus_tag="P5558_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23858.1"
                     /translation="MSKRSAVAGWLLGLGPLVLAAGQALAASEVGQSTADIVHEAEHE
                     SGGMPQLDASTFATQILWLIISFVVLYYLLKRKALPRVADILEARQERISADLDRAAA
                     LRVDAEAAFEQYEAVVAEAQAKAQAEIKATREAIAADVAGRTATLDRDLAARIAAAER
                     TVAAARDKALGELEGVAVEVAQAATERLIGVQVGDEEARAALARARQEVA"
     gene            6359..6832
                     /locus_tag="P5558_05825"
     CDS             6359..6832
                     /locus_tag="P5558_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRY23859.1"
                     /translation="MIEFWLLVALAILIALVWKPFRQHVLGGLDARSDKIRAELEEAK
                     RLHEEAKQLLARHQRQLHEGESLAADIRSRAAAETKRLETQLRADFEQLVQRRTKLAE
                     ERIAQEEARAVADVRARAAELAVATTRRLLTEKLGGEAAQRVMQNSIREVTQKLG"
     gene            6844..7359
                     /locus_tag="P5558_05830"
     CDS             6844..7359
                     /locus_tag="P5558_05830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY23860.1"
                     /translation="MSGDRAGLVIRPYDAADWPAVWRIVEPVIRAGETYPEPADLDEA
                     AARSWWIEAHRAVFVAVDRADGTLLGTYYLTDNKPGQGAHVANAGFMVGPGARGRGVG
                     RAMGAHSLEAARALGYLAMQFNLVAVTNTASLRIWDALGFTRVGLLPHAFRHPSQGLV
                     DAWVLYRWLGD"
     regulatory      7380..7597
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7696..8376
                     /locus_tag="P5558_05835"
     CDS             7696..8376
                     /locus_tag="P5558_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRY23861.1"
                     /translation="MHMEPGVVTGAQSLLGVATATGALGYAGRRALEALRIENPAALA
                     LRSVIATGLVLTFFEVLPHHPVGVSEVHLILGSTLFLLFGLVPTAIGLALGLLTQGLL
                     FAPFDLPQYGMNVTTLLVPLFAVAALARRIIPAGTPYVDLGYGDVLKLSVTYQGGICT
                     WVAFWSLYGGGFGAANLQAVGVFFAAYMLVVLLEPLVDLAVLALAKAGRNTAGGPLFH
                     RRLLQGAG"
     gene            8407..8814
                     /locus_tag="P5558_05840"
     CDS             8407..8814
                     /locus_tag="P5558_05840"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRY23862.1"
                     /translation="MAADASIGSARPPSIDELAAIGERILNELPEGIRELLGPVPVMV
                     QDWPDDALLDALEIGDPLELTGLYQGVPISQRHAALPPSEPEMIFLFRLPILVEWCER
                     GCPLEEVVFDVLTHEIGHHLDMDEAWVLRMEGR"
     gene            9171..9446
                     /locus_tag="P5558_05845"
     CDS             9171..9446
                     /locus_tag="P5558_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRY23863.1"
                     /translation="MTLDDLNQAVANATGTTKAGAAKSVQAVLASIQEALTKKEKVSI
                     AGFGVFETVDRPARDGRNPQTGQTIKIAASTAIKFKPGKTLRDKVNA"
     gene            complement(9504..10220)
                     /locus_tag="P5558_05850"
     CDS             complement(9504..10220)
                     /locus_tag="P5558_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY23864.1"
                     /translation="MGERSGIGGILFDKDGTLIDFRATWLAAYRGAAAELCLAAGLAS
                     DFATTLLDRSGYDSRTDSFAATSPLLWATNTAIAESWAAEPEVRGRPGVVELVLAHFD
                     DAERYPAVAVGDLVALFGRLRGRGLRLGVATMDSTAKAEAAVARLGLGPSLDFVTGYD
                     GGHGEKPGPGMVRGFCAATGLEPGEIVVVGDTAADLQMARAAGAGVAIAVLTGATPRD
                     LLDALADHVLESVMQIEEVI"
     gene            10396..10878
                     /locus_tag="P5558_05855"
     CDS             10396..10878
                     /locus_tag="P5558_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRY23865.1"
                     /translation="MLDDPLQPADAGLIPAEAAGRADVFLYGTLAHEEVLAAVLDRPI
                     VAGELVPAVLAGYRREAVIGASYPLLVAHAAAEVAGRLLRRPTLRELRRINHFEADEY
                     LAEELPVSVAGRQQSAWVFLALEAMVPSGRAWELEQWAAMHLPAFRQRVVAWMKEAPR
                     "
     gene            10962..11921
                     /locus_tag="P5558_05860"
     CDS             10962..11921
                     /locus_tag="P5558_05860"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRY23866.1"
                     /translation="MRLLILSLLLAGSLAAAAPAAEPLVSADWLEGQLGGAGVRIVDI
                     RNGGADETFDQGHVPGAVHGEYGTAGWRTDVAGVPGMTPEVASLEALIGGLGIDNESH
                     VVIVYSGADASDFGAAARVYWTFNYLGHDDVSILDGGWRGWTEGGDRPVATGPASVTP
                     ATFTADVRPDFLIETAEVEATLDDPAVLRLDARPRSQYIGETKAGPARAPGRLPDALG
                     LEQAVFFTDDGRLKERGEIAALVPAEVTDGGVETVVSYCNTGHWAATNWFVLSELLGQ
                     ENVRLYDASMTGWSADPARPLEIGGESLLAQVSRWVGAPCATC"
     gene            11908..12705
                     /locus_tag="P5558_05865"
     CDS             11908..12705
                     /locus_tag="P5558_05865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY23867.1"
                     /translation="MQPAEPSARDAQAALAAAYAKLFEGLLLLGPGSVETRRLVLGLV
                     RAALPPNPVIADMGAGAGAASRFLADALPAAKILAVDRASVILGALGEADPGRVIAVN
                     GDMAAPPLAAASLDLVWCESAIYAVGRGTALAAWRRLLRPKGLVVFSDVAWREPPAGR
                     PPEAVAFWSETYPEMQDADGIRAEIEAAGYAVTARHWAPASDWADYYAPLRQRLDDLA
                     PGATPPLAGVLDEMRREVALFDAHGASYGVVFFVVYPALNRPFTDRR"
     gene            12742..13695
                     /locus_tag="P5558_05870"
     CDS             12742..13695
                     /locus_tag="P5558_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HRY23868.1"
                     /translation="MRLAYSISGYKLPGQLAWLMTAIRHPADLFVLHVDARTPASVLD
                     EMRGAAGEGPNVVFIERQAITWMGISLVEAELRAVRAALAADPPFDYLISLSMQDYPL
                     KSRAEIVAALGAAPGLDHVTRERLDGLPFHIRRRPWLVAFERRGRLVKTPIPRPVPKG
                     LELRWKGSWWRVLSRQTCEWLVQADLTRRYLDFLRHVQAPDELFFQNLLMQGPHRDRL
                     AERNRHFVAWSGIGGSPKTLTMAEAEELLASPLWFARKFDEKVDRRVLELLAQRIGAD
                     LPPHVARPCHRAMDAAGVRADRPGVTGTDPPALAGIAVAAR"
     gene            complement(14100..14801)
                     /locus_tag="P5558_05875"
     CDS             complement(14100..14801)
                     /locus_tag="P5558_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23869.1"
                     /translation="MVAAATQDATPIDRHARVVHLTMPHTGRPHAPAATSVDTKRTPI
                     SIQMIEIARFFYYSQAKIKNGFDLRLFLVFILSQKGKIMSKLEKATFSQRWSALQPSK
                     TVLVWACLGSVAATMVLGFTWGGWVTGGTAREMAEDAGASSRYELASVICVEKFLNAD
                     DARAELTTFQAIKSSYKQREFIEAGGWAVMPSKDKAARQSADLCAKVLANLDPAELSV
                     PAVEAAVTEPAAVVQ"
     gene            14880..15128
                     /locus_tag="P5558_05880"
     CDS             14880..15128
                     /locus_tag="P5558_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23870.1"
                     /translation="MEEVLRWLGMGAAVIAAIIVSARLGQKITGIGFVVFLVSSTAWV
                     AVGLMAGEHALLIQNGVLTIVNVVGIWRWLVRPALEGG"
     gene            15214..15477
                     /locus_tag="P5558_05885"
     CDS             15214..15477
                     /locus_tag="P5558_05885"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HRY23871.1"
                     /translation="MARDHGPTIKDDATYEALRDEGASKEKAARIANARANPKQHPSR
                     KGGKSPPYEEWTKQQLVRRARELDVEGRSSMNKAELIAALRDR"
     gene            15601..16491
                     /locus_tag="P5558_05890"
     CDS             15601..16491
                     /locus_tag="P5558_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23872.1"
                     /translation="MSGRKAASRLVAILWLLALGLLANPAAAQHRSPDFRNSPARTAQ
                     AFKRDVFIENLRRGNRAVVLGWFRHGPGFTFNIKKTAKAAGYSSTAPAYADILGDPEA
                     NFLKCEGGPFALCFYSGPEGPLPCDTSKYDTISECTCTVIDHGLYFVDILGILNLDVY
                     EATVAACGPDGSGCPDIDDAPVCDVVNEKLDPKKKMFPADMISVFSLDGAAGVIGQSN
                     CESGKYAGCMTAPCYYPNGDEESDTVQCLCPNYSGPFQIGQDDKEDMCSLGNHHVWSA
                     AYTLPGGNIIPDGPPLTHRR"
     gene            16496..17266
                     /locus_tag="P5558_05895"
     CDS             16496..17266
                     /locus_tag="P5558_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY23873.1"
                     /translation="MTNEATRRLAVLIDADNTSPRIAEALFAEVAKIGEASVRRIYGD
                     FSGTRLKAWADILSRHAIIPHQQFAYTKGKNASDIALVIDAMDLLLSGRFDGFCLVSS
                     DSDFTRLAARIREQGTDVFGFGEKKTPESFVQACRRFIYTENLLAAAADAPADDAAAP
                     PPAESSDAALAILDRVIADLDQDDGWVFLSLVGSRLANLAPDFDPRTYGHKRLGDLVK
                     ATDAFEMATETGRGTRIRRKPAKKKTGSRRRKSNPEPV"
     gene            complement(17358..17690)
                     /locus_tag="P5558_05900"
     CDS             complement(17358..17690)
                     /locus_tag="P5558_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRY23874.1"
                     /translation="MATEDEALSGELVDETARLDLDELCALCGVQADHLVEMVEAGVI
                     RPEGDRQTVWRFSAVAVMRSRKALRLRRDLDINLPGLAVTLDLLDEVDRLQNELKSLR
                     RRLGQLQD"
     gene            complement(17694..18656)
                     /locus_tag="P5558_05905"
     CDS             complement(17694..18656)
                     /locus_tag="P5558_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRY23875.1"
                     /translation="MQLKDYYKIIGVAEDATADDIKKAYRKLAREHHPDLNKGDGSAD
                     RFKEVGEAYEVLKDPAKRKEYDEIRKYGRRQGEAFRPPPGQGAGPAGGGFDEADLEDF
                     LQSIFRQRPAGNGAGGRAGGRAGPGGARFAIPGEDIHYRIGVTLEESYKGATRSLALQ
                     THRFDEAGHVLPDTRTLNVRIPKGVVEGQRIRLKGQGGAGFGGAPNGDLYLEVELLPH
                     PRFTVDGRDVSLALPVAPWEAGLGATVKVPTLGGPVSLAIKPGAQSGQRLRLAGRGLP
                     GKPPGDQYVVLKIVMPKVAGDADRALLEQMQEQMSFDPRATLGV"
     gene            18857..19345
                     /locus_tag="P5558_05910"
     CDS             18857..19345
                     /locus_tag="P5558_05910"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRY23876.1"
                     /translation="MQVRTHPLILALPVLLAASPAPAIEVQRHIDVSAPAEEVWSLVG
                     DFCSIEQWHPEVTGCELEEDDATTFRTLRTADGSTIREQLLDHDAAIRFYSYSLVESP
                     WPVTGLVSEFQLAPGAVGENVIVWQAQYSTLAVDKDAAEKSLGAFYRNGLEGIAGMFT
                     DE"
     gene            19426..20202
                     /locus_tag="P5558_05915"
     CDS             19426..20202
                     /locus_tag="P5558_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HRY23877.1"
                     /translation="MANLAGILGMLLATRMAGRGRYGSALGTAALLRGGLARKAGLAG
                     LGYLAYRAYQDGKADRAAAGGADGPAGRDAARESSGPGAAAGGGIAGTLGNLLDSLTG
                     GQVASRKGEPGLGDKLGNYFDPGSAEPAGETAAPPEAARQPMDDGRALLLIRAMIAAA
                     NADGRISDEERRRVLAAVDEADGDAGDHRLIEHELLDPKPLDSLLAEVGDHDTARQLY
                     AVSRAAMDGETLTQIAYLDYLRNRLGLPPDETAEIDEAMQ"
     gene            20281..21273
                     /locus_tag="P5558_05920"
     CDS             20281..21273
                     /locus_tag="P5558_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRY23878.1"
                     /translation="MDDRRFGTVDSRGHWAPNEPVSYGPLFTWPPQARALLKWFFGFP
                     GFLLPWNLLYAVAAVLIWAFLTPSLETMRTAAPGWMLLVLARNLVLAIAVYGAWHLWL
                     YVWRKQDTSFKYNRQWPKAGVSTFMLGSQNRENMFWTLASGVPIWTAYEVLLLWAYAS
                     GTITILSPTEHPVAFVALFFLVPFIHEVGFYFCHRLLHWPPLYRVAHALHHRNTNPGP
                     WSGLSMHPIEHVIYFSTILLFFLFPAHPIHMINLASRLGLAPAQGHTGFDRVVVGDDK
                     TMNTSYFAHYLHHKYFEVNYADGMVPLDKWFGSFHDGTPEAHALMKARRARRGV"
     gene            21413..22438
                     /locus_tag="P5558_05925"
     CDS             21413..22438
                     /locus_tag="P5558_05925"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY23879.1"
                     /translation="MALMLCLTSGAAALAQDVNDAPLTESWAPTEWGADDMAGSVNRI
                     TPELVLDAVGLVKQGKVATLGKVYAGDAPAFGSRGWRMTMPGLPTGGPFGSHQLVYND
                     EYLATEIGQIGTQFDGPGHIGVITSDGLFFYNGRLLDKDPAIGAYGLGPLGVEHVAEK
                     GFVCRGVLLDAVAYRGGQLPIPTGAEGDPGIITDADIEAMVQAQGIEPIGEGDCVFLY
                     TGHGDIWHPSDWDKFDAAEKTARTAEFNSGEPGFGISACEYLAERKIILTGADTWAVE
                     AVPGENADKPFECHMQMQTRRGIWNLENLDLSQLVADGAHEFLFVWAPLKMKGATGSP
                     GNPMALY"
BASE COUNT         3359 a         7860 c         7996 g         3403 t
ORIGIN      
        1 tcgtgcgtct ccgcgtcagc ggcttcaagt cgttctgcga tgcgaccgag ctcgatatcg
       61 ggcacggcct gacgggcatc gtcgggccga atggctgtgg caagtcgaac atcgtcgagg
      121 cgctgcgctg ggtcatgggc gagagctcgg ccaggggcct gcgcggcggc gagatggacg
      181 atgtcatctt cggcggctcc gccatgcggg cccccttcga cctcgccgag gtggcgctcg
      241 ggctgaagca ggcgccgcgg gccgcgggcg acgccgccat ccacgtcctg ccgagctccg
      301 aggatctgga gatcagccgg cgcatcggcc ggggcaccgg ctcgctcttc cgcctgaacg
      361 gccgcgaggt gcgggcccgc gacatccagc tcctcttcgc cgatgcgggt gccggggcca
      421 agagcccggc catcgtcggc cagggccagg tcggcttcat cgtcgaggcc aagccggagg
      481 agcgccgccg cctgctcgag gaggcggccg gcatcggcgg catgcacagc cgccggcgcg
      541 aggccgagct caagctcgcg gcgacggaga gcaatctcga gcggatcacc gaccagctcg
      601 gcctcctggc ggagcggcgc acggcgctgg agaagcaggc gcgcgaggcc gagcgctaca
      661 agaagctgca gggccagatc cgggcgctcg atgcctggct ggtcctggcg cgcatcaccg
      721 ccgggcgcac ggctctcgcg gcggcgaagg aggccctggc gctggcccgc ggccggctgg
      781 gcgaggagcg gacccgggcg gccgccgccg aggcggcgca tgacgaagtg gtgcgtcggc
      841 tgccggattt gcgcgaccgg gccagcgtgg cgctggcgct gcgcgcacgg ctcgcggagc
      901 gggtcgagca ggtcgagcgg atcgccgccc aggagcaggc cgagcgggcg cggctccagg
      961 ccgatcgcga cgacacgcag ggccggatcg ccgcgctggg cgaggcgatc gacgccggca
     1021 atgcgcggca cgacaccatc ctcgcgaccg accgcaagct ggcctcgacc atcgagaccc
     1081 tgacggccga gcgggccgcc atcggcccct cgagcgatgc cgccgcccag cggctgcagg
     1141 cgctgggcca ggaggtgcag gaggcgagtg cccgccgggc cgagctggag cggcggctcg
     1201 agggggcccg ggcccggcac gaggagcgcc accggcggct gcggtcggtc gaagccgagc
     1261 tcctgcggct cgccgatgtc gccgacgacg cgctggtcga ggcggccttg cgcaagcgcg
     1321 acgaggcggc aggcctgctc gccgtggccg atgccgagct cgcggcggca caggcggagc
     1381 gggacgcggc ggccgcaccg ctcgaccggc tgaaggcgga ggtggaggcg gcgagcgagg
     1441 cggtccggca ggcggcggcg agggtcgcct cgctcgatgc cgagatcggc cgccggcgca
     1501 cggcgctcgc cggcctcgcc gagcgccggc agtggcacga gcgggccctc gggcaggcga
     1561 cgcgcgccgc ggcggaagcc gacgaggcgc tggccgccct cgccccggag cagccggacg
     1621 aggacgggga gctggcggcg aagctgggag cgctcgaggc gcgcgcggca gcgctggaga
     1681 cggagctcgc cgacgcggtg ccagaggtcg cccgggcgcg cgaggccctg gccacggccg
     1741 agtgcggcct cgccgcgcat gatgcggagc ggctgaagct ggcggccgag gccgaggcgc
     1801 tggcgctgat cgatgcgccg gacgccgagg caccggtgct cgatctcctg cgcgtcaccg
     1861 ccggttgcga gacggcgctg gcagccgccc tcggcgacga cctgatggcc gggctggacg
     1921 aggcggcggc gacacactgg caggaggtgc cgggcgagat agccgccccc gacctgccgc
     1981 ccggggccgt gccgctcgcc gagcgggtcg aggcgccggc ggcgctgatg cgccggctgc
     2041 ggcaggtcgg cctcgtcgcc gaccgggcca cggcccttcg cctgctgccc gccctggccc
     2101 agggccagcg gctggtcacg gcggatggcg ggctctggcg ctgggacggc ttcgtgcgcg
     2161 gtgcggaggc cgacgacacc atgcgccgtc ggctggccca gcggcagcgg cgccagatgc
     2221 tgcaggcccg cctggcgggg ctggatggcg accgcgcggc gctggcccgg gtggtcgagg
     2281 tcgccaccgg agagctggca tcggcgcagg atggcgaggt ggcactcgcg aaggagcggg
     2341 cggagctggc gacggcgatc gaccgggccg cccaggcgcg cgagacggcg gccgtggcgc
     2401 gacggcaccg gcaggcggag cgccagcggc tgaccgagac gcaggcgcgc agccgggcag
     2461 agcgggcgga gcacgaaaag gcgctggccg agctcgaggc ggcgggcgag gcgcatgcga
     2521 tcgaggcggt ttccggcgag gcggcgacgg cccgggcggc gctggccgag gccgaggagc
     2581 aggcgaagac gctcgtggcc gcgctcggcg ccgcgaccag ggcgcatcag gcggccgcca
     2641 gccgcgccgg cgaggccgag gcgagccagc ggcgggctgc ccaggacgcc ctcgccgccc
     2701 gcctgctgca cgagcggcgg cagggcgagg cggagctgag gaagagccat cgggccgacg
     2761 cggtgaccgg cctcgacacc gaggcgcggc aacttcgcgg cgagctcgag gggctggacg
     2821 aagagatcga ggcgggcacc accgggctcg cggcggccac cgccaccagc gagcggctcg
     2881 ccgccagcca tgccgaggcc ggaatcgagg ccgagcggct gcgcacgcgc cgggcggcgg
     2941 tcgatgccga ggagcggagt gcccaggacc agcgccgggc gctggccgac gagctggcgc
     3001 acctgatcgc ggatctcgac cggcgggggg cggacctcgt ggctacccgg cagcggctgc
     3061 agactgtcga gcaggagctg gcccgggcgg tggcggccag ccccggcgac gatccgggtc
     3121 tggccctgcg gcgggagctg gaggccgccg agtgccaggc gagcgaagcc gcctcggcac
     3181 tcgccgcggc cgaggccgag ctgcaggctt gcgaggcgca gcggcgcacc gccgatgcgg
     3241 acctcgcgag ggcgcacgag gcctcggccc tggcgctcgc cgatgtggag cggctgggcg
     3301 aggcgcagcg cgagctcgag cgggagacca cgcgccggct cggccagccg atcgaggcgg
     3361 ccctcgccgc cgccgaggag agcggggtcg agcgctcgct cgagggcgag ccgccggacg
     3421 tgctggaggc gcggctcgag aagctgaaga cgtcgcgcga gcggctgggt gccgtcaatc
     3481 tccgggcggc gctggaatgc gaggagctgg ccgccgagat cgccgggctc gagcacgagg
     3541 ccggcgagct gcggcaggcg gtcgagcggc tgcagcgggc gatctcgacg ctcaatcgcg
     3601 aggcacgcgg ccggctcgag gcggtgttcg acgatgtcga ccggcatttc cggcagctct
     3661 tccagcggct cttcggcggc ggcaaggcgc atcttcgcct gaccaacatg gacgacccgc
     3721 tcaatgccgg cctcgagctc gaggcgatgc cgcccggcaa gaagctgcag aacatccgcc
     3781 tgctctccgg tggcgagaag agcctgacgg cgctggcgct ggtcttcgcc ttcttcctca
     3841 cccagccctc gccgctctgc gtgctggacg aggtcgatgc cgccctcgac gacgccaatg
     3901 tcgagcgctt cgccgacctg ctggaggaga tggcgaagga gacgaagacc cgcttcctcg
     3961 tggtcaccca tcatcccctg accatggccc ggatggatcg cctcttcggc gtcaccatgg
     4021 ccgagcgcgg cgtgtccaag ctggtctcgg tcgccttcga cgaggccgag cgcctgcgcg
     4081 ccaccgcctg agtgtgcgcc tgccgcactg cggcaacctg acggtgccgg cgggtgcttg
     4141 aaggattgac acccctgcga ggcatttcta ggttcgcggc gcctctgccg gggcgtttgc
     4201 tgtcgcgtgc gcgcggtgct ggcgctgttg tggaggtgag ggacggatgc cggaggacgg
     4261 gaaaccaccc gggttcagcg atttcgatgc caggctggcg gccacgcggc aggcgcgttc
     4321 ggacaagcgg acgagcgacg acgcagcgga gcggccgcat ttcgactggg gtcgggggat
     4381 gcaggccggg atcgaggtcg tcgccggtgt cgccggtggc agcctgctcg gttgggcgct
     4441 cgatgcctgg ctgggcacgg gtccgtttct gttcatcggc gggttcatgc tcggtgctgc
     4501 ggccggaatg ctcaatgcat tccgcaatct gcgccggtgg atgggcgggg acggcaacgg
     4561 tccggcccag gggcagtgat gacaggcgac cagggcagtg gcccggtcca gggcggagtg
     4621 gggtaaccgg tggcaggtcc tctcgagcag ttcgagatca agccgatcgt ccgcatcctg
     4681 atcgagggtg tcgatctctc gttcaccaat tcggcactct ggatggtgat cgcggtggcg
     4741 gctgtctact acttcgtggt gctgggcacc cggcagcacg cgctggtgcc ggggcggctg
     4801 caatcgctcg tcgaactgtc ctacgagttc gtcgccgggc tggtccgcga gaacgcgggc
     4861 aaggagggca tgcggttctt cccgctgatc ttcacgctct tcatgttcat cttcatgggc
     4921 aacatgctgg gcatgatccc cggcagcttc accttcacca gccacatcat cgtcaccttc
     4981 ttcatggcgc tctgcgtgtg ggtcggtgtc acggtctacg gcttctacct gcacggcgcc
     5041 cggttcctca ccttcttcgc gccgagcggt gcgccggtct acatgctgcc gctgctcgtg
     5101 ccgatcgaga tcctgtccta ctgcgtccgg cccatcagcc tctcggtccg gctcttcgtc
     5161 aacatgatgg ccggccacac catgatgaag gtgttcgccg ggttcatcat cgctctcggc
     5221 gtgttcggcg tggcgccctt ggcgatcgcc gtggcgctga ccggcttcga gttcgccgtg
     5281 gcgttcctcc aggcctacgt cttcaccgtg ctcacctgcc tctatctgca cgacgccatc
     5341 cacatgcact gaatcgacca gcgcccgttg ggggcgctgc ccaggaccac atttccaaac
     5401 caacgaaccc gagtggagtt gatcgatgga actcgaagct gcgaagatga tcggcgccgg
     5461 tcttgcgacc attgcgctct tcggtgtcgg tatcggcatc ggcaatgtgt tctcgaccct
     5521 gatctcgacg gtcggccgca acccctcggt gcagcagcgg gtcttcccgt tcgccatcat
     5581 cggcttcgcg ctggtcgagg cggtggcgct ctttgcgctc ctgatcgcct tcctcatcct
     5641 gttcacctga agctctgggc gatcagcggc ggcgccggcg agcggtggcc gtcgccatgg
     5701 ccgtcgcgtg aacgagggtg gtgatgatga gcaagagatc tgcggtcgcc ggctggctcc
     5761 tcggcctcgg gccgctggtg ctggccgccg gccaggcgct ggccgccagc gaggtcgggc
     5821 agtcgacggc cgatatcgtg cacgaggcgg agcacgagag cggtggcatg ccacagctcg
     5881 acgcttcgac cttcgctacc cagattctct ggctgatcat cagcttcgtc gtcctctact
     5941 acctgctcaa gagaaaggcg ctgccgcgcg tcgccgacat cctcgaggca cggcaggagc
     6001 ggatctccgc cgatctcgac agggcggcag cactcagggt cgacgccgag gcggccttcg
     6061 agcagtacga ggcggtggtg gccgaggccc aggccaaggc gcaggccgag atcaaggcga
     6121 cgcgtgaggc gatcgccgcg gacgtggccg gccgcaccgc cacgctcgac cgtgacctcg
     6181 ccgcccggat cgccgccgcc gagcggaccg tggccgcggc ccgtgacaag gcgctcggcg
     6241 agctcgaggg ggtcgccgtc gaggtggcgc aggcggcgac cgagcgcctg atcggtgtgc
     6301 aggtcggcga cgaagaggcc agggcggcgc tcgcccgggc acggcaggag gtcgcctgat
     6361 gatcgagttc tggctgctcg tcgccctcgc catcctgatc gccctggtct ggaagccctt
     6421 ccgccagcat gtgctgggcg gtctcgatgc ccgctcggac aagatccgcg ccgagctgga
     6481 ggaggccaag cgcctgcacg aggaggccaa gcagctcctc gccaggcacc agcggcagct
     6541 ccacgagggc gaaagcctgg cggccgacat ccgctcccgc gctgccgccg agaccaagcg
     6601 gctggagacc cagcttcgcg ccgacttcga gcagctcgtg cagcgtcgca ccaagctcgc
     6661 cgaggaacgc atcgcccagg aggaggcccg cgccgtcgcc gacgtgcgcg cccgggcggc
     6721 cgagctggcc gtggccacca cgcgccggct gctcaccgag aagctcggcg gcgaggccgc
     6781 ccagcgggtg atgcagaatt cgatccgcga ggtcacgcag aagctcggct gaccggccct
     6841 gccatgagcg gggatcgggc cgggctggtc atccgcccgt acgacgccgc ggactggccg
     6901 gccgtctggc gcatcgtcga gccggtgatt cgcgccggcg agacctatcc cgagcctgcg
     6961 gacctcgacg aggcggcggc gcgaagctgg tggatcgagg cgcaccgggc cgtcttcgtc
     7021 gccgtcgacc gagcagacgg cacgctgctc ggcacctact acctgaccga caacaagccg
     7081 ggccagggtg cccatgtcgc caatgccggc ttcatggtcg ggccaggagc gcgcggccgc
     7141 ggggtcggcc gggcgatggg cgcccacagc ctcgaggccg cgcgcgcgct cggctatctc
     7201 gccatgcagt tcaacctggt ggcggtgacc aacacggcct cgctgcgcat ctgggatgcc
     7261 ctgggcttca ccagggtcgg tctgctgccg cacgccttcc gccatccctc gcagggcctc
     7321 gtcgacgcct gggtgctgta tcgctggctg ggtgattgac tcgcctgacg atttctgtgc
     7381 cgattcgcgc tgttgttggt gtcacccagg cgggcgcgtg cccggctggg tgatgaaacg
     7441 ggaagccggt tcgcgcaatc tcaagccggc gctgcccccg caacggtagg tgagtgaaga
     7501 cgggccacga tgccactgcg cgttgtcgcg ggaaggcggc ccgtccggtg atgtccgctc
     7561 acgagcccgg agaccggcca gcaacgagag cgcggcattg cccggggcgt gggcagagat
     7621 tcccgtcccg gtgatgcctt cttcggcaac ctccgcacgg ggtgcaactt tgcggacaga
     7681 ggagaggacc acgacatgca tatggaaccc ggcgtcgtca ccggcgcgca atccctgctc
     7741 ggcgtcgcca cggcgacggg tgccctcggc tatgccggca ggcgcgcgct cgaggccctg
     7801 cggatcgaga acccggcggc gctggcgttg cgctcggtca tcgccaccgg cctcgtgctc
     7861 accttcttcg aggtgctgcc gcaccatccg gtcggcgtct ccgaggtgca tctcatcctc
     7921 ggctccaccc tcttcctgct cttcggcctg gtgccgacgg caatcggcct ggcgctcggc
     7981 ctcctgaccc agggcctcct cttcgccccc ttcgacctgc cgcaatacgg catgaacgtg
     8041 accacgctgc tggtgccgct cttcgccgtc gccgccctgg cgcggcgcat catcccggcc
     8101 ggcacgccct atgtcgacct gggctacggc gacgtcctca agctctcggt cacctatcag
     8161 ggcggcatct gcacctgggt ggcgttctgg tcgctctatg gcggcggctt cggtgccgcg
     8221 aacctgcagg ccgtgggcgt cttcttcgcc gcctacatgc tggtcgtgct gctcgagccg
     8281 ctggtcgatc tcgccgtgct ggcgctcgcc aaggccggcc gcaacacggc tggcggcccg
     8341 ctcttccacc ggcggctgct gcaaggcgcc ggctgagccg cagcaccggc cgccacggga
     8401 gggaacatgg cagcggatgc gtcgatcggc agcgcccggc cgccgtcgat cgacgagctc
     8461 gcggcgatcg gcgagcggat cctgaacgag ctgccggagg gcatccgcga gcttctcggt
     8521 ccggttccgg tgatggtgca ggactggccc gacgatgccc tgctcgatgc gctcgagatc
     8581 ggtgatccgc tcgagctcac cgggctctat caaggcgtgc cgatcagcca gcgtcatgcc
     8641 gcgctgccgc cgagcgagcc cgagatgatc ttcctcttcc gcctgcccat cctggtggag
     8701 tggtgcgagc gcggctgccc gctcgaggag gtcgtgttcg acgtgctgac ccacgagatc
     8761 ggccatcacc tcgacatgga cgaggcgtgg gtgctgcgca tggagggccg atgagcgaag
     8821 ggccggaatg ccgtagtggt gccgaggggc cgctgctctc gctctctatc cgctttgcgg
     8881 cggacactcc cgtcgaggtc gcccggcccc tggagtcggg agaggcgagc gccggcgaac
     8941 cggcaagcgc ttgaaagcgc ttggtttcga cattcgggcg cgggttgccg gagccgcccc
     9001 cgcggtggcc gggcggcaat acccgaagag gggttatcgc cttatggttt tgccacaaca
     9061 gccggtttca aaggctccag gcctctcaac tcgacatttt tcgggtattc gcaaccaaaa
     9121 ctattcatga tacagcgccg tgcagacttt atgaacaagg gagtgtgttc atgaccctcg
     9181 acgatttgaa tcaggccgtc gccaacgcca ccggcacgac caaggccggc gccgcgaagt
     9241 cggtccaggc cgtgctcgcc agcattcagg aagcgctgac gaagaaggag aaggtctcga
     9301 tcgccggctt cggcgtgttc gagacggtcg accgcccggc ccgtgatggc cgcaaccccc
     9361 agaccggcca gaccatcaag atcgccgcca gcaccgcgat caagttcaag ccgggcaaga
     9421 cgctgcgcga caaggtcaac gcctgatcga agtcgatggg ctgcagcgcc gtcgggcccc
     9481 ggccgggcgg cgcagcgctt tcttcagatc acctcctcga tctgcatcac gctctcgagc
     9541 acatgatcgg ccagggcatc gagcagatcg cgcggggtag cgccggtcag cacggcgatc
     9601 gcgacccccg cccccgccgc tctcgccatc tgcagatcgg ccgccgtatc gccgaccacc
     9661 acgatctctc ccggttcgag gccggtcgcg gcacagaagc ccctgaccat gcccggcccg
     9721 ggcttctcgc catggccgcc gtcatagccg gtgacgaaat cgaggctggg tccgaggccg
     9781 agcctcgcca ccgccgcctc cgccttggcg gtgctgtcca tggtggcgac gccgagcctg
     9841 aggccacgcc cccgcagccg cccgaagagg gccacgagat cgcccaccgc gacggcagga
     9901 tagcgctcgg catcgtcgaa atgcgccagc accagctcga ccacccccgg ccgaccgcgc
     9961 acctccggct cggccgccca gctttcggcg atcgccgtgt tggtcgccca gagcagcggg
    10021 ctggtggcgg cgaagctgtc ggtccggctg tcgtagccgc tgcggtcgag cagcgtggtc
    10081 gcgaagtcgg aggcgaggcc ggcggcgagg catagctcgg cggcagcacc ccgataggcg
    10141 gcgagccagg tggcgcggaa gtcgatcagc gtgccgtcct tgtcgaagag gatgccgccg
    10201 atccccgacc gctcgcccat ccgcatcgct ccctctgccc gcctgggcat gaacagctcg
    10261 tcttcctgcc cggggatagc agatttggga tgcaagcggg atggcttcgc gcaggtcggg
    10321 catcttgcgc atggcgggca gcggcgcgat atcgggcgac tggcagaggt gaaggggaac
    10381 gggggctctc cggtcatgct cgacgatccg ctgcagccgg ccgatgccgg cctcattccg
    10441 gcagaggccg ccggccgtgc cgatgtcttc ctctacggca cgctggcgca cgaggaggtg
    10501 ctggccgccg tgctcgatcg gccgattgtc gcgggcgagc tggtgccggc cgtgctcgcc
    10561 ggctaccgcc gcgaggccgt gatcggcgcc agctatccgc tcctggtcgc ccatgccgcg
    10621 gccgaggtgg cggggcgcct gctgcgccgg cccaccttgc gcgagctccg gcgcatcaac
    10681 catttcgagg ccgacgaata cctggccgag gagctgccgg tcagcgtcgc cggccggcag
    10741 cagtcggcct gggtgttcct ggcgcttgag gcgatggtgc cgagcggccg agcatgggag
    10801 ctcgagcagt gggcggcgat gcacctcccc gcctttcgcc agcgcgtcgt ggcctggatg
    10861 aaggaagcgc cgcgctgaaa ttattacaat aatattttgt attgaatttc aattaatctc
    10921 gattaaatgg gaaattgata attatccagt gaggtttcgc catgcgcctg ctcatccttt
    10981 ccctgctgct cgccggctca ctcgccgccg cggccccggc cgccgagccc ctggtttccg
    11041 ccgactggct cgagggccag ctcggcggtg caggcgtgcg catcgtcgat atccgcaatg
    11101 gcggtgccga cgagaccttc gaccaggggc atgtcccggg cgccgtccac ggcgagtacg
    11161 gcacggccgg ctggcgtacc gatgtggccg gcgtgcccgg catgacgccc gaggtggcga
    11221 gcctcgaagc cctcatcggc gggctcggca tcgacaacga gagccacgtg gtgatcgtct
    11281 acagcggcgc cgatgccagc gatttcggcg ccgccgcccg ggtctactgg accttcaact
    11341 atctcggcca tgacgacgtc agcatcctcg acggcggctg gcgcggctgg acggagggcg
    11401 gcgaccggcc ggtggcgacc gggccggcga gtgtgacgcc cgcgaccttc acggccgatg
    11461 tccgccccga cttcctgatc gagaccgccg aagtcgaggc cacgctcgac gatccggccg
    11521 tgctgcgtct cgatgcgcgg ccgagatcgc agtacatcgg cgagaccaag gccgggccgg
    11581 cgagggcgcc gggccgcctg ccggatgcgc tcgggctcga gcaggccgtc ttcttcaccg
    11641 atgacggccg gctgaaggag cggggcgaga tcgccgcact ggtgccggcg gaagtgaccg
    11701 atggcggggt cgagaccgtg gtcagctact gcaataccgg ccactgggcg gcgaccaact
    11761 ggttcgtcct ctcggagctg ctgggccagg agaacgtgcg cctctacgac gcctcgatga
    11821 ccggctggag cgccgatccc gcgcggccgc tcgagatcgg cggcgagagc ctgctggcgc
    11881 aggtcagccg ctgggtcggg gccccttgtg caacctgctg agccatccgc tcgcgacgcg
    11941 caggctgccc tggcggcggc ctacgcgaag ctgttcgagg ggcttctcct cctcggcccc
    12001 ggctcggtcg agacgcggcg gctggtgctc gggctcgtcc gggccgccct gccgccgaac
    12061 ccggtcatcg ccgacatggg ggcgggagcc ggagccgcga gccggtttct cgccgacgcc
    12121 ctgccggcgg cgaagatcct cgccgtggat cgggcatcgg tgatcctcgg agcactcggc
    12181 gaggcggatc cggggcgggt gatcgccgtc aacggtgaca tggcggcacc gcccctggcc
    12241 gccgccagcc tcgatctcgt ctggtgcgag tcggccatct acgccgtcgg ccgcggcacg
    12301 gccctcgccg cctggcggcg cctgctccgc cccaagggct tggtcgtttt cagcgatgtc
    12361 gcctggcggg agccgccggc cgggcggccg cccgaggccg tggccttctg gtccgagacc
    12421 tacccggaga tgcaggacgc ggacgggatc cgggccgaga tcgaggcggc gggctatgct
    12481 gtaaccgccc ggcactgggc accggcctcg gactgggccg actactacgc gccactgcgc
    12541 cagcggctgg acgatctggc acccggggcc acgccaccac tagccggcgt gctcgacgag
    12601 atgcgccgcg aggtggcgct tttcgatgcg cacggggcga gctatggcgt cgtctttttc
    12661 gtcgtctatc cggcgcttaa ccggcccttt accgatcggc gctagagttc cccgaatcca
    12721 tagcgcggat caccggagcc catgcgcctc gcctactcga tcagcggcta caagctgccg
    12781 ggccagctcg cctggctgat gacggcgatc cggcacccgg ccgacctttt cgtcctgcat
    12841 gtcgacgcgc gtacgcccgc aagcgtgctg gacgaaatgc gcggggcggc gggcgaaggg
    12901 ccgaatgtcg tcttcatcga gcggcaggcg atcacctgga tgggcatctc gctggtcgag
    12961 gcggagctgc gggcggtccg tgccgcgctc gccgccgacc cgcccttcga ctacctgatc
    13021 agcctgtcga tgcaggacta tccgctgaaa agccgggccg agatcgtggc ggcgctcggg
    13081 gccgcacccg ggctcgacca cgtcacccgc gaacggctcg acggcctgcc cttccacatc
    13141 cgccggcggc cctggctcgt ggccttcgag cgccggggac ggctggtcaa gacaccgatt
    13201 ccgcggcctg tcccgaaggg cctcgagctg cggtggaagg gctcctggtg gcgcgtgctc
    13261 agccggcaga cctgcgagtg gctggtccag gcggacctga cccggcgcta tctcgacttt
    13321 ctccgccacg tccaggcgcc ggacgagctc ttcttccaga acctgctcat gcaggggccg
    13381 caccgcgacc ggctcgccga gcgcaaccgg catttcgtcg cctggtccgg catcggcggc
    13441 agcccgaaaa cgctgaccat ggccgaggcg gaggagcttt tggcatcgcc cctgtggttc
    13501 gcccgcaagt tcgacgagaa ggtggaccgg cgggtgctcg agctcctggc ccagcggatc
    13561 ggcgccgacc tgccgccgca tgtggcccgg ccgtgccacc gggcaatgga cgccgccggg
    13621 gtgcgggccg accggccggg tgtgaccggc acggacccgc ccgcgttggc aggaatcgcg
    13681 gtggccgcac ggtgacggcc cgctatagcg gaagaggtgc tggcgagccc gcacgggggc
    13741 tcgatccggg acggtggcgc cgtgccttgg ttgggccgga gcgggtcgcc gggtgccggc
    13801 ggcctcccgg cggcaggcga ccccggcagg ttgccgtcca agagcctgtc cgagatgcgg
    13861 ccggcatgat cgggaggcgg aacgtgccag ggctgaccgc gttgcgcacc ggcggccacc
    13921 ttcctcgctc gaccaggctc aacccgaccg ggccgacgcc cggcaatccc gttgggtcgg
    13981 cggggtcgtc gccgggcatt cggctcggcg ccacgcgaaa acgctccgtg gcaggggcca
    14041 tggagcgtca tcgaagagtg gccgccgggt gccggcaccc ggtggcgcta tggaacgacc
    14101 tattgaacga ccgcggcagg ctcggtcacg gcggcctcga ccgcagggac cgacagctcg
    14161 gccgggtcga ggttggccag gaccttggcg cagagatcgg cggattgccg ggctgccttg
    14221 tccttgctcg gcatgaccgc ccagccgccg gcctcgatga actcgcgctg cttgtaggag
    14281 ctcttgatcg cctggaaggt cgtgagctcg gcccgggcgt catcggcatt caggaacttc
    14341 tcgacgcaga tgaccgaggc gagctcgtag cgggacgaag cgccggcatc ctcggccatc
    14401 tccctggccg tgccgccggt gacccagccg ccccaggtga agccgaggac catggtcgct
    14461 gccaccgagc cgaggcaggc ccagaccagc accgtcttcg acggctgcaa cgcgctccag
    14521 cgctgtgaaa aggtagcctt ctcgagtttt gacatgattt ttcctttctg agataaaata
    14581 aatactaaaa acaacctcaa gtcaaatcca ttcttaattt tggcttggga ataataaaag
    14641 aaacgagcaa tttcaatcat ctggatagat attggggttc tttttgtatc gacggaggtg
    14701 gcggctggcg cgtgcgggcg tccggtatgc ggcatggtca ggtgcacgac ccgggcatgg
    14761 cggtcgatcg gtgttgcatc ttgggtagcg gcagccacca tgggcaccac gggcgatgcc
    14821 cggccgttca gttgtcggcc gggcagaggc tcgtcgggcg ggcatgggga ggcatggcaa
    14881 tggaggaggt cctgcgctgg ttggggatgg gggccgcggt gatcgccgcg atcatcgtct
    14941 cggcgaggct cggccagaag atcaccggga tcggcttcgt cgtcttcctg gtctcgtcca
    15001 ccgcctgggt cgcggtcggg ctgatggccg gcgagcacgc gctgctgatc cagaacggcg
    15061 tgctcacgat cgtcaacgtc gtcggcatct ggcgctggct ggtgcggccg gcgctggagg
    15121 gtggctgaac cccgcgcctg gaaccgagcg gcctgtcggt cgttcgagcg tcgtgtggca
    15181 ttcggcacca gcaatcgcgg aggcgagaag gcgatggcga gggaccacgg accgacgatc
    15241 aaggatgacg cgacctacga ggcgttgcgt gacgagggtg ccagcaagga gaaggcggca
    15301 cgtatcgcca atgcgcgggc gaacccgaag cagcacccga gccgcaaggg cggcaagagc
    15361 ccgccttacg aggaatggac caagcagcag ctcgtgcggc gcgcccgcga gctcgatgtc
    15421 gaggggcggt cgagcatgaa caaggccgag ctgatcgccg ctcttcgtga tcgctgagag
    15481 cctgctcgag agctccccgc cggcacggca tgcctgagtg ccgcgacaac cgactggatt
    15541 tggggtcaac tttcccggga aaagcgcgta tgcctcgacg aaagatcggg gagggcaggc
    15601 atgtcggggc gcaaggcagc gagtcggctg gtggcgatcc tctggctcct ggcactcggc
    15661 ctgctcgcca acccggccgc ggcccagcac cggagcccgg atttcaggaa cagcccggcg
    15721 cgcacggcgc aggccttcaa gcgcgacgtc ttcatcgaga atctccgccg gggcaatcgg
    15781 gcggtcgtgc tcggctggtt ccgccacggc ccgggcttca ccttcaacat caagaagacg
    15841 gcgaaggccg cgggctactc atccaccgca ccggcctatg ccgacattct cggcgacccg
    15901 gaggccaatt tcctgaaatg cgagggcggg cccttcgcgc tctgcttcta ctcgggccct
    15961 gaagggccgc tgccctgcga taccagcaag tacgacacca tctccgaatg cacctgcacg
    16021 gtgatcgacc acggcctcta cttcgtcgac atcctcggca tcctgaacct ggacgtctac
    16081 gaggcgaccg tcgcggcctg tggccccgac ggcagtggct gcccggacat cgacgatgcg
    16141 ccggtctgcg acgtggtcaa cgagaagctc gatccgaaga agaagatgtt cccggccgac
    16201 atgatctccg tcttcagcct cgacggtgcc gcgggtgtga tcggccagtc gaattgcgag
    16261 tccggcaagt atgccggctg catgaccgcc ccttgctatt atccgaatgg cgacgaggag
    16321 agcgacaccg tgcagtgcct ctgccccaac tattccggtc ccttccagat cggccaggac
    16381 gacaaggagg acatgtgctc gctcggcaat caccacgtct ggtcggcagc ctacacgctc
    16441 cctggcggca acatcattcc ggacggcccg cccctgacgc accggcgcta gcggcatgac
    16501 caacgaagcc acccgccgcc tcgccgtgct catcgacgcg gacaacacct cgccgcgcat
    16561 cgccgaggcg ctcttcgccg aggtcgccaa gatcggcgag gcgagcgtgc gccgcatcta
    16621 tggcgacttc tcgggcaccc gcctcaaggc ctgggccgac atcctctcgc gccacgccat
    16681 catcccgcac cagcagttcg cctacaccaa gggcaagaac gcctcggaca tcgcgctcgt
    16741 catcgacgcc atggacctgc tgctgagcgg ccgcttcgac ggcttctgcc tcgtctcctc
    16801 cgacagcgac ttcacccgcc tcgccgcccg catccgcgag cagggcaccg acgtcttcgg
    16861 cttcggcgag aagaagacgc ccgaaagctt cgtccaggcc tgccgccgct tcatctacac
    16921 cgagaacctc ctggccgccg cagccgacgc gccagccgac gatgccgccg ccccgccgcc
    16981 cgccgaatcg tccgacgcgg cgctggccat cctcgaccgg gtcatcgccg acctcgacca
    17041 ggacgacggc tgggtcttcc tctcgctcgt cggcagccgc ctcgccaacc tcgcccccga
    17101 cttcgacccg cgcacctatg gccacaagcg cctcggcgac ctcgtcaagg ccaccgacgc
    17161 cttcgagatg gcgaccgaga ccggccgcgg cacgcggatc agacgcaagc cggcgaagaa
    17221 gaagactggg tcgcgcaggc ggaaatccaa ccccgagccg gtctgaccga gtcgaggcga
    17281 cgaccatgga aagcgaggag gatgatcctc ctcgcgctcc accggtgttt ggacctgcag
    17341 ccggaccggc gggcgggcta gtcctgcagc tgcccgaggc gccggcggag cgacttcagc
    17401 tcgttctgca gccggtcgac ctcgtcgagc aggtcgaggg tcacggcgag accgggaagg
    17461 ttgatgtcga gatcgcggcg cagcctgagc gccttgcgcg agcgcatgac cgcgaccgcg
    17521 gagaagcgcc agacggtctg ccggtcgccc tccggccgga tgacgcccgc ctcgaccatc
    17581 tcgaccaggt ggtcggcctg cacgccgcag agcgcgcaga gctcgtcgag gtcgagccgg
    17641 gcggtctcgt ccacgagctc gccgctgagg gcctcgtctt cggttgccat ctctcagact
    17701 ccgagggttg cgcggggatc gaagctcatc tgctcctgca tctgctcgag gagcgcgcgg
    17761 tccgcgtcgc ccgcgacctt cggcatgacg atcttcagga cgacgtactg gtcgcccggc
    17821 ggcttgccgg gcaggccgcg cccggcgagc ctgagccgct ggccggactg ggcgccgggc
    17881 ttgatggcga gactgaccgg gccgcccagc gtgggcacct tgacggtggc tccgaggccg
    17941 gcttcccaag gcgccaccgg cagggcgagg ctcacgtccc ggccatccac ggtgaagcgc
    18001 ggatgcggca ggagctcgac ctccaggtag agatcgccgt tcggcgcccc gccgaagccg
    18061 gccccgccct gccctttcag gcggatcctc tggccctcga cgacgccctt gggaatcctg
    18121 acgttcaggg tgcgcgtgtc cggcagcaca tggcccgcct cgtcgaaacg gtgcgtctgc
    18181 agtgccaggc tgcgggtggc gcccttgtag gattcctcga gggtcacgcc gatgcggtag
    18241 tggatgtcct cgcccggtat cgcgaagcgt gcgccacccg gcccggcccg gccaccggcc
    18301 cttccacccg ccccgttgcc ggcagggcgc tggcggaaga tcgactggag gaagtcctcg
    18361 agatcggcct cgtcgaagcc gccgccggct ggcccggcac cctggcccgg cggcggccgg
    18421 aacgcctcgc cctgacgcct gccatacttg cggatctcgt cgtattcctt gcgcttcgcc
    18481 ggatccttga ggacctcgta ggcctcgccc acctccttga accggtcggc ggagccgtca
    18541 cccttgttga gatccggatg atgctcgcgg gcgagcttgc gataggcctt cttgatgtcg
    18601 tcggccgtgg cgtcctcggc gacgccgatg atcttgtaat agtccttgag ctgcatcgcc
    18661 tgtcccggtc ggctcctgca cccccactga aggggtgccg ctcctgatac gccgccgggc
    18721 cgcggccctc aaggccgggg tcggcgacga ccgccgggaa cgccccgcgc gctgcgccgt
    18781 tcagtcgttg tcggccgccg gcgcttcggg gcgccgggga gccgcattcc agcgagcgat
    18841 gtgaggagag acgatcatgc aagtccgcac gcacccgttg atcctggccc tgcccgtcct
    18901 gctggcagca agccccgcgc cggcgatcga ggtccagcgc cacatcgacg tttcggcgcc
    18961 tgccgaggag gtctggagcc tggtcggcga cttctgctcc atcgagcaat ggcatccgga
    19021 ggtgaccggc tgcgagctcg aggaggacga cgccaccacg ttccgcaccc tccgaaccgc
    19081 cgatggcagc accatccgcg agcaactgct cgaccacgac gccgccatcc ggttctacag
    19141 ctacagtctc gtcgagagcc cctggccggt gacgggcctg gtcagcgagt tccagctcgc
    19201 ccccggagcg gtcggcgaaa acgtcattgt ctggcaggcc cagtactcga cgctcgcggt
    19261 cgacaaggac gcagccgaga agtcgctcgg cgccttctac cggaatggcc tcgaaggcat
    19321 cgccgggatg ttcaccgacg agtgacccct ggcgcgcggt gctggaacat ccacgatcct
    19381 ggcgcgttct tcctgccaca cacatcgcac ggggaagggg cgaacatggc caatctcgca
    19441 ggaatcctcg gcatgctgct ggccacccgc atggccgggc gcggccgcta tggctcggcg
    19501 ctcggcacgg cggcattgct gcgcggcggg ctcgcccgca aggccggcct cgccgggctc
    19561 ggctacttgg cctaccgggc ctaccaggac ggcaaggccg atcgcgcggc agccgggggc
    19621 gccgacggtc ccgccgggcg agacgccgcg cgggaatcga gcgggccggg tgccgccgct
    19681 ggcggcggca tcgccggcac cctgggcaac cttctcgaca gcctgaccgg cggccaggtc
    19741 gccagccgca agggcgagcc cggcctcggc gacaagttgg gcaactactt cgacccgggc
    19801 tcggccgagc cggccggcga gacggcggca ccgccggagg ccgcgcggca gccgatggac
    19861 gacggacggg cgctcctcct catccgggcg atgatcgccg ccgccaacgc cgatggccgc
    19921 atctccgacg aggagcgccg gcgtgtcctg gcggcggtgg acgaggccga cggcgacgcc
    19981 ggggaccacc gcctgatcga gcacgagctt ctcgacccga agcccctgga cagcctgctg
    20041 gccgaggtcg gggatcacga caccgcccgg cagctctacg ccgtctcgcg ggccgccatg
    20101 gacggcgaga ccctgaccca gatcgcctat ctcgactatc tcaggaaccg cctcggcctg
    20161 ccgcccgacg agaccgccga gatcgacgag gcgatgcagt aggggcagcc ccttcttgca
    20221 cgcagccgtg ttcccgatag gttgtggagc gggaagagaa acggcgctcg gggacgggac
    20281 atggacgaca ggcgattcgg gacggtggac agccgcggcc actgggcgcc caacgagccg
    20341 gtcagttacg gcccgctctt cacctggccg ccacaggccc gcgctctcct gaaatggttc
    20401 ttcggctttc cgggctttct tctgccctgg aacctgctct acgcggtggc cgccgtgctg
    20461 atctgggcgt tcctcacgcc ctcgctcgag accatgcgca ccgccgcgcc cggctggatg
    20521 ctcctcgtgc tggcgcgcaa cctcgtgctg gccatcgccg tctacggcgc ctggcatctg
    20581 tggctctatg tctggcgcaa gcaggacacg agcttcaagt acaaccgcca gtggccgaag
    20641 gccggtgtct cgaccttcat gctcggcagc cagaaccgcg agaacatgtt ctggacgctg
    20701 gccagcgggg tgccgatctg gacagcctac gaggtgctgc tgctctgggc ctatgccagc
    20761 ggcaccatca ccatcctgag ccccaccgag cacccggtcg ccttcgtcgc cctcttcttc
    20821 ctggtgccct tcatccacga ggtcggcttc tacttctgcc accgcttgct gcactggccg
    20881 ccgctctacc gcgtcgccca cgcgctgcac caccgcaaca ccaatcccgg cccctggtcg
    20941 ggcctctcca tgcaccccat cgagcacgtg atctacttct cgaccatcct gctcttcttc
    21001 cttttcccgg cccacccgat ccacatgatc aacctggcat cccgcctcgg cttggcgccg
    21061 gcccagggcc ataccggctt cgaccgcgtc gtggtcggtg acgacaagac gatgaacacg
    21121 tcctatttcg cacactacct gcatcacaag tatttcgagg tgaactacgc cgacggcatg
    21181 gtcccgctcg acaagtggtt cggctccttc cacgacggca cgcccgaggc gcacgcgctg
    21241 atgaaggcaa ggcgggcgag gcggggggtg taggcggcac cggcaattcc gtgtttaccg
    21301 ggcagcggca ttgcgtgcat gctcgtggcc gctgccgcac cggaaagggg ccggcaggaa
    21361 cgatgaaccc gagggaggac gagacatgcg actgtctgat cgaagcatcg gcatggcact
    21421 catgctctgc ctgacttccg gcgctgcggc tctggcccag gacgtcaacg acgcgccctt
    21481 gaccgagagc tgggcaccca ccgaatgggg tgccgacgac atggccggct ccgtcaaccg
    21541 catcacgccg gagctggtgc tggatgccgt gggcctggtg aagcagggca aggtcgcgac
    21601 cctgggcaag gtctacgcgg gcgacgcgcc ggccttcggc agccgtggct ggcgcatgac
    21661 catgccgggc ctgccgaccg gcggcccgtt cggctcgcac cagctcgtct acaacgacga
    21721 gtacctggcc accgagatcg gccagatcgg cacgcagttc gatggcccgg gccatatcgg
    21781 cgtgatcacc tccgacggcc tcttcttcta caatggccgc ctgctcgaca aggacccggc
    21841 gatcggcgca tacggcctcg gcccgctcgg ggtcgagcat gtcgccgaga agggcttcgt
    21901 ctgccgcggc gtgctcttgg atgccgtggc ctatcgcggc ggccagcttc ccatcccgac
    21961 cggtgccgag ggcgaccccg gcatcatcac cgacgcggat atcgaggcca tggtccaggc
    22021 ccagggcatc gagccgatcg gcgagggcga ctgcgtcttc ctctacaccg gccacggcga
    22081 catctggcac cccagcgact gggacaagtt cgacgcggcc gagaagacgg cgcgcaccgc
    22141 cgagttcaac tcgggcgagc ccggcttcgg catctcggcc tgcgaatacc tggccgagcg
    22201 caagatcatc ctgaccggcg ccgacacctg ggcggtcgag gccgtgcccg gcgagaacgc
    22261 cgacaagccc ttcgaatgcc acatgcagat gcagacccgc cgcggcatct ggaacctcga
    22321 gaacctcgac ctctcccaac tggtcgcgga cggcgcccac gaattcctct tcgtctgggc
    22381 cccgctcaag atgaagggtg cgaccggctc gcccggcaat ccgatggcgc tttattgata
    22441 ggattacgag gaatggcggg gaggatgatc ctccccgccg gcccctccgg ttcttagggt
    22501 ggatgaaggt tggggtggtc gggaaaacgt ctgggaagcc tggacggcgg agtggggttg
    22561 gcacggagag ggttgaagtc ggttcgtgtc cctacttctc cactccaagc aatctttg
//
LOCUS       DAOTFI010000041        22543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2715_length_22543_cov_6.280683, whole genome shotgun sequence.
ACCESSION   DAOTFI010000041 DAOTFI010000000
VERSION     DAOTFI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22543
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2715_length_22543_cov_6.280683"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="P5558_05930"
     CDS             <1..497
                     /locus_tag="P5558_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY23880.1"
                     /translation="LLDHPARPALVAADTVSAAKAMRLADHLARLDLLFTNAAEAAAL
                     VGPGAPAELARRLVAKGVGAVVLGQGAAGLALADHNGVHALDAHTVPVVDVTGAGDAL
                     AAAVLAARLAGLDLAAAGRIGRLASALAVASDEAVPKDFGIPALRDLALRLDSEAHVQ
                     LARL"
     gene            475..1446
                     /locus_tag="P5558_05935"
     CDS             475..1446
                     /locus_tag="P5558_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRY23881.1"
                     /translation="MSSLLAFSPEVAAARKAGQPIVALESTIIAHGLPRPRNLETARL
                     LEAEVREAGAVPATIAVADGRLRIGLDAAQLEELAVADGVLKVSRRDLAGCIATGRTG
                     ATTVAATMVAAQLAGIRAFATGGIGGVHRGAERDFDVSADLEELARTPVAVVCAGAKS
                     ILDLPKTLEVLETKGVAVVGYRTDEFPAFFCRASGLAVPLRLDSVAEIANLLDVQRRL
                     GLTSGVLVVNPIPKAAALPEELVEGAIAEALAVATAAGIGGKQITPFLLARIVELTGG
                     QSVEANVALALSNVRLAAEITVALHALGEPILPTPRIRPRSRQMDAM"
     gene            complement(1428..2204)
                     /locus_tag="P5558_05940"
     CDS             complement(1428..2204)
                     /locus_tag="P5558_05940"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRY23882.1"
                     /translation="MIDAVDASFDLKGRRALVTGASRGIGEALAIGLARFGADVAVTA
                     RDVAGLADVVAAIEEQGRKAVPVAMEARSVDGIRRGVAEAAAALGGLDLVVVNAGTEE
                     VCASLEVEEGLWDRIHGTNLKGAFFTAQAAARLMGAAEGGTILNICSLTSAVGVPTAA
                     PYGASKAGLLGLTRTLATEWAPLGIRVNAIGPGYFRTALTEMFYADEAWQRAMLAKIP
                     AGRFGDLRDLVGSAVFLASDAARYVTGQILYVDGGYMASI"
     gene            2383..3447
                     /locus_tag="P5558_05945"
     CDS             2383..3447
                     /locus_tag="P5558_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23883.1"
                     /translation="MVRLSGVGKTFGGTVAVDAVSLDLAAGRFLTILGPSGCGKTTLL
                     RMIAGFIAPTTGRIEVNGAAVESVPPWRRSIGMVFQRLALFPHLSAFDNVAYPLRMRR
                     FPAAGIAERVERYLELVRLPGMGGRRPHELSGGQQQRVAIARALVFEPDILLLDEPLA
                     ALDRKLREDMQLEFRRIQQELGVTTINVTHDQREALVMSDEVMVMHQGRVQQSAPPAE
                     IYRQPANRFVAGFLGLTNFLDAEVLRAEGERRVVRIGGAELSGTGNVATGARVTAALR
                     AEKLRLLAPGAVAETQFPATVTEVVFEGDRILYEVRVDGLGNARLQLFDHDPEGHGRH
                     SPGDIVTIGWSSAHLLLFTD"
     gene            3476..4576
                     /locus_tag="P5558_05950"
     CDS             3476..4576
                     /locus_tag="P5558_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PotD/PotF family extracellular solute-binding
                     protein"
                     /protein_id="HRY23884.1"
                     /translation="MTKPLTTIGRRRLLQAAGLGTTALATPWLWRRPAMAQEKPAELI
                     VRAWGGVWVEALDKGVSQPFTEATGIAIRHDLTEDNEIRPRIWAAVDQGRVPPIHVNW
                     DTSTNATKSSLRGVTEDLGDLPNLADMLPAATPAGFDGVPMINMYSYVYVLAYRDEAF
                     PDGPPVSWREMLEPRFKGRVALYNDGIGFHPAAQIAGGGTLEGIPDDMEPCWAFIREL
                     SAQEPLLGEDPDFTAWFQNGEIDIACTILSNARAAKQNGIAVSWTVPEEGAKVDTDGM
                     WIPKGLPGNELHWAKEYVNFAMSQAAQQEWTSLLGLPGLRPGLTAPADLAGDPAYPQT
                     PEDFARLISVPTRILVENESDWFQKFQEIMQG"
     gene            4682..4936
                     /locus_tag="P5558_05955"
     CDS             4682..4936
                     /locus_tag="P5558_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRY23885.1"
                     /translation="MHTVNMLEAKTQLSKLVEAVESGAEAEIVIARNGKPAAKLVPIG
                     VEKTKRRPIGFLDGKYPSTSQEEFDSTNDEIYKMFFGEEE"
     gene            4933..5322
                     /locus_tag="P5558_05960"
     CDS             4933..5322
                     /locus_tag="P5558_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY23886.1"
                     /translation="MKLLLDTQIAIWSISGSERLSGQMREMLAAGSNDIFVSSVSIWE
                     IAIKFPLRKPSAPPFSASEAVRHCRTIGYTLLSITPEHAAAVGTLPHHHGDPFDRLLI
                     AQALTEPLRLVTADRRLAAYSDTVIPC"
     gene            complement(5319..5915)
                     /locus_tag="P5558_05965"
     CDS             complement(5319..5915)
                     /locus_tag="P5558_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRY23887.1"
                     /translation="MWLDDEDEKKKPDDGAKAGGMLEERLFKSRTILIAGQVEHKMAK
                     LVAAQLLALADDGDDWINVFVSSPGGHVESGDMIYDMLKFVGPRVRTIGTGWVASAGA
                     LIFVGAARENRFCLPNTRFLLHQPSGGMMGSASDIGIQAEQIIKMRERLNRIFANETG
                     QPIERIAKDTDRDHWMFPEEAMAYGLLHKVIRTKGELG"
     gene            6065..6946
                     /locus_tag="P5558_05970"
     CDS             6065..6946
                     /locus_tag="P5558_05970"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23888.1"
                     /translation="MRATLFPRGAGGLTPWLFLLPALLLTFLLVAGIVPLLDTSLRQL
                     DRATFRPGEDWTLANFHTLWERPVYTWLAARTMGAALVVTVLSLLLALPYAWLMVRTP
                     RPWLKKLLLVSLFLPFFIGQVVRAYGWLVVLGNQGLANAVLGWLGLPPVTILYTLPGV
                     VLGLVQYMLPFAVLMLAPAFTAVPEEIELASGSLGASPARTFRHIVLPLVRPGLVAAA
                     VVVFTLTLTDFAMPAIMGGGRNDFIANAIYDAFFSLSDPGLGSALAILLTLAGTTIAG
                     IMLALFGLGTLGYVRRG"
     gene            6946..7749
                     /locus_tag="P5558_05975"
     CDS             6946..7749
                     /locus_tag="P5558_05975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23889.1"
                     /translation="MQRSPFTLLLGLVVAFDLVFLAVPTLVVVVISFTSGNMVAFPPE
                     GFSLRWYGVLLTRPEFLAALGRSLQVGLVCTALAIPAGTLAALALARWRIRYPTGLQL
                     YLLLPFTVPLVVSGIALMLLFGRMGMLGQVWPVGLAACVINLPFMIWAVAASVNHLDP
                     DLENAAMNCGAPPTHTFFTVTLPAVAPGILTGALVMFILAVNEFLVSLMLVDARIVTL
                     PVLVYNAIRNIITPDLAAVSVVYLLIAVIAIWLLDRLVGLGILLESKRS"
     gene            7758..9071
                     /gene="hisD"
                     /locus_tag="P5558_05980"
     CDS             7758..9071
                     /gene="hisD"
                     /locus_tag="P5558_05980"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRY23890.1"
                     /translation="MINLFELDQLSTAEREALLLRTEADLSAFMQPVARIVDRVRDGG
                     DAAIAELAREIDGVTLDPAGLEATEAEFAAADAALDQDVKDAIAYATDNIRRFHERQK
                     PEDMWLMEIQKGAFAGERWSPIRSVACYVPRGKGAFPSVMMMTTIPAVVAGVAEIIVL
                     TPPTPEGGIDAASLHAARMAGVRRVFRVGGAVAVAAAAYGTATIPKVAKIVGPGSPWV
                     VAAKRHLADRIDTGLPAGPSESIVLADATANPDIAALDLLIEAEHGADSSAYLVTDSR
                     EVAERARARLPELIANLGAERRGYIEAVLGGKCGGIVLAPSWDEAVRFTNDYAPEHLE
                     ILSKEPMQHLGAITDAGEILLGENTPMTIANFCLGPDCVLPTGGWARTFSALGVQDFM
                     KRTSLGHVTRAGFAEPARHAAVLARYEGFEAHAQALGPIRDTFRP"
     gene            9158..9541
                     /locus_tag="P5558_05985"
     CDS             9158..9541
                     /locus_tag="P5558_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23891.1"
                     /translation="MRQARRYGGRWAGRAALVALLLQLAQPWLGLQPVRAEVGPTGPV
                     LVICTGSGLLRIYADGTPVQETDRQGLHCTACVARLHGPALLPTAPALRLPRVATPVA
                     TRVSAPGTAARATAPPLPARGPPTV"
     gene            9635..10123
                     /locus_tag="P5558_05990"
     CDS             9635..10123
                     /locus_tag="P5558_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRY23892.1"
                     /translation="MLRWILSGTLALAAIGTVQANELTVGEITIDHPWARATAAQQAN
                     GAAYLVVDNGGEADRILGAASPVAGRVELHNHIDDNGVMRMRRVEAIDLPAGETTALA
                     PGGLHIMLFELGAPLVVGETFPLTLTLEQAGAVEVEVTVEPVSYGIGGDAKGHQGHGS
                     GG"
     gene            complement(10170..10727)
                     /locus_tag="P5558_05995"
     CDS             complement(10170..10727)
                     /locus_tag="P5558_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRY23893.1"
                     /translation="MAAAAPAGADADVRLVRVWDPFVRLFHWALVVFFTAAWLTGEEI
                     DWLHELLGYGVLALVGMRVVWGFIGSPHARFRDFAFGRATTLAYARDALAMRAKRYLG
                     HNPLGAAMVLALLAGLVVTGGSGWLLTTDLLGHEPRWLEETHEVAANLMLVLIATHIA
                     GVIFSSLSHGENLVRAMVTGMKRAE"
     gene            complement(10727..11002)
                     /locus_tag="P5558_06000"
     CDS             complement(10727..11002)
                     /locus_tag="P5558_06000"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRY23894.1"
                     /translation="MRKLLMLSTALVPMALALPALASGPDCADVPRAQWMTEDALRAK
                     LQGMGYEVREIDREGNCYEVEGRDANGFEVEAYVDPVSGAVVPGEDD"
     gene            11129..11434
                     /locus_tag="P5558_06005"
     CDS             11129..11434
                     /locus_tag="P5558_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRY23895.1"
                     /translation="MTRTRLALLASLLASLPLAKAAADSDHERARQALERGEVLPLAE
                     ILERVRSQAPGEVIETEFDREGGRWVYELKLIDPQGRVVELEVDAVDGRILRSELDD"
     gene            11431..12102
                     /locus_tag="P5558_06010"
     CDS             11431..12102
                     /locus_tag="P5558_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY23896.1"
                     /translation="MRLLLVEDDRRIARDVIALLEAQGHLVDHATDGEDAWFKGDVED
                     YAAVVLDLGLPGMDGLAVLKRWRENGRTMPVLVLSARGTWKERVEGIDAGADDYLPKP
                     FEPEELAARLRALIRRSAGQGRPVLEIGDVALDPRAMRVSVKGVPRALSPQEFRLLDY
                     LMLQAGRVVPPSELLEQLYGDEDSRETNTIEVLVGRLRKKLGVELISTRRGFGYTIER
                     DDPAP"
     gene            12099..13484
                     /locus_tag="P5558_06015"
     CDS             12099..13484
                     /locus_tag="P5558_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY23897.1"
                     /translation="MSSQSLRLRLLLAQAASVVVALVLAGIGLTVLFERHLERRVDQE
                     LATYVRQLAAHLVIDADGALNLEAPLADPRFAVPLSGLYWQIEDQGTGTVLRSRSLWD
                     DVLPLPVDEPEPGQAHRHPLPGPGGSLLVVHERLVLLGNGPGERRIRIAAAVDRATLD
                     AAGTDYARQLAIALAGLALVLVAAAAVQVWYGLRPFEAVRRAVTAVREGRAKRLDGRF
                     PDEIRPLVQEVDALLEAQEGSLARARQRAADLAHGLKTPLTVLGSEARGLAARGETKA
                     AAELDRLARDMRRHIERELARTRLAGATSRGQATALAPAIDRLVRTLRRTPAGEARDW
                     RLDVPAHLLVAVEADDLGELLGNLLDNAAKWARQSVTVQAVADAGDVRLAIADDGPGV
                     DAAELDQLGTRGHRLDTGKPGHGIGLAISRDIALAYGGTIAFANHPDGGLRVSLTLPA
                     AQLPTGQASMR"
     gene            complement(13451..14119)
                     /gene="tenA"
                     /locus_tag="P5558_06020"
     CDS             complement(13451..14119)
                     /gene="tenA"
                     /locus_tag="P5558_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HRY23898.1"
                     /translation="MAFTDDIWTRTGELRRAIDALPFNRELAAGTLSRERFRFYMLQD
                     KLYLDQYARALTLAAGKAPDLAATEKFAGSAMGALKVERALHGTFLERFGVSAAELAA
                     ALPSPSCLAYTNFLLATAHQESWPVLVAAILPCFWIYHDVGTRIAQGSAAGNFYQPWI
                     DTYASPDFARSVEEVIAITDMAAERAGADERERMAAAFIRSAQYEWLFWDSAYRMEAW
                     PVGS"
     gene            14190..14690
                     /locus_tag="P5558_06025"
     CDS             14190..14690
                     /locus_tag="P5558_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HRY23899.1"
                     /translation="MTFFGATLRGTLALGLFLAAGSAAAETIGDVSTKFHWISPDHKI
                     VVEAFDDPKIEGISCYLSRAKTGGFKGAFGVAEDTSDASITCLQVGPITVKDELDEGE
                     EVFKRRTSLIFKSMQVVRFLDEKRSTFVYLVYSDRVIEGSPKNSISAVPAMPWGNVQP
                     DLAKAR"
     gene            complement(14700..16055)
                     /locus_tag="P5558_06030"
     CDS             complement(14700..16055)
                     /locus_tag="P5558_06030"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HRY23900.1"
                     /translation="MIKLFFAQALLAEGWARDVAVEVAPDGTFHSVEPNAVPAPDAAR
                     AAIAVPGMANVHSHAFQRGMAGLAERAGPAEDSFWTWREVMYGFLDRLTPDDVQAIAG
                     QLYAEMLEAGFTAVGEFHYLHNDTDGRPYADPAELAERIVAAAAATGMALTHLPVLYH
                     QGSFGAAPPAPGQRRFVMDVEDLLELVRTLRARHPDLVVGAAPHSLRAVTPESLRRLV
                     EACTAGPLHIHAAEQEKEVLDCLAWSGRRPVRWLLDEVGLDGRWCLIHCTHLDDGEVR
                     DLAASGAVAGLCPVTEANLGDGIFRGVDYLGRGGRFGVGSDSHIRIDLADELRSLEYS
                     QRLRDRRRNRLAAPGHSVGRTLLEKALAGGAQALAQNTGAIAVGRRADIVTLDAGHPA
                     LVGRSGDALLDGWIFAARTAPVDTVRVGGRLVVEGGRHVAGEAVARRFERTMRGLLAD
                     A"
     gene            16129..17328
                     /gene="hutI"
                     /locus_tag="P5558_06035"
     CDS             16129..17328
                     /gene="hutI"
                     /locus_tag="P5558_06035"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRY23901.1"
                     /translation="MLLTNARFATMQSGGAPYGALDDAALLIEAGRIAWLGPLSAAPR
                     AAETADLGGRWVLPGLVDCHTHIVFGGNRAREFELRLEGATYEEVARAGGGILSTVKA
                     TRAASVDELVAQALPRLDHLLAEGVTTIEIKSGYGLELESEIRMLEAARRLADERDVE
                     VQTSLLAAHAVPPEFKGRADAYVDIVVSQIIPAVRKAGLADAVDGFCEGIAFSPAQMR
                     RVFEKARAVDLPVKLHAEQLSDLKGAVLAAGFGALSADHLEYVQEDGVRAMAAAGTVA
                     VLLPGAFYTLREKQAPPVDLLRRHGVPIAIATDCNPGSSPVTSLLLVLNMAATLFRLT
                     PEEALAGVTRNAARALGLEAEIGTLEVGKRADLAIFDIDHPAELAYRIGYNPLFASLR
                     EGRWRPC"
     gene            17322..18848
                     /gene="hutH"
                     /locus_tag="P5558_06040"
     CDS             17322..18848
                     /gene="hutH"
                     /locus_tag="P5558_06040"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRY23902.1"
                     /translation="MLTLRPGAVTLAEWRAVLAGTPVTLDPACRSAVRASAEAIRTIV
                     AENRTVYGVNTGFGKLASTRIAKGDLELLQKNLVLSHSTGTGPPLPDPLVRLIMAMKA
                     ASLARGYSGISEGTLDQLLALLAKGVLPVIPEKGSVGASGDLAPLAHLTAALLGVGEA
                     RLHGTTLPAGKALAEAGLAPVTLGPKEGLALLNGTQVSTALALAALLRIETLFRTALV
                     TGAMSVDAARGSDAPFDARIHAIRGHPGQIAVAAAFRAFLHESPIRASHVDCGKVQDP
                     YSLRCQPQVMGAVLDQLRMAAGVLTIEANAVSDNPLVFAESGDVLSGGNFHAEPVALA
                     ADQIAIAMAEIAALAERRIAMLTDGSISGLPPFLVADPGLNSGFMIAHVTAAALASEI
                     KQRTHPASVDSLPTSANQEDHVSMATHAARRLHAMADDLEGIIAIELLAAGQGLDVHL
                     PLTTSSRLLAPHRTLRAHVATWDKDRFFAPDIEAARRLVAEGAVIKDSLDILPGLETS
                     "
     gene            18862..19656
                     /gene="hutG"
                     /locus_tag="P5558_06045"
     CDS             18862..19656
                     /gene="hutG"
                     /locus_tag="P5558_06045"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HRY23903.1"
                     /translation="MITVDVEERGTPLLLSLPHTGTGLPKGLAERLTEAGRALVDTDW
                     HVDRLYAFADATGATTVKAGFSRYVIDVNRDPSGQSLYPGQATTGLCPTTTFDGEPLY
                     LSGEEPDAAEIEDRRQRWFEAYHQALAAQIARLKERFGTVRLYDCHSIRSVVPRLFPG
                     TLPVLNLGTNEGRSCSPALRAQLVAVMAESGLTHVADGRFKGGWITRHYGDPSNGIEA
                     VQMELAQSAYMDEAPPWPYRADLAARLEPTLRRLLETLLAAKGEKT"
     gene            19653..21320
                     /locus_tag="P5558_06050"
     CDS             19653..21320
                     /locus_tag="P5558_06050"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRY23904.1"
                     /translation="MNPRLDNARKIKAPHGTTLRAKSWLTEAPLRMLMNNLDAEVAEH
                     PESLVVYGGIGRAARDWQAFDRIVASLETLEDDETLLVQSGKPVGVFRTHPDAPRVLI
                     ANSNLVPHWATWDHFNALDRQGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEVGRRHY
                     GGKLDGKWILTGGLGGMGGAQPLAATMAGASMLAVECRPSRIEMRLNTGYLDTATESL
                     DEALSILDRSCRQKEPISVGLLGNAAEIFPELVRRGVRPDVVTDQTSAHDPLHGYLPA
                     GWSLEEWEERQESDPQGVTAAAKESMVLQVQAMLDFHHQGIPTLDYGNNIRQMAKEEG
                     LAGAFEFPGFVPAYIRPLFCRGIGPFRWAALSGDPEDIYKTDAKVKALIPNDRHLHHW
                     LDMARERIRFQGLPARICWVGLGLRHKLGLAFNDMVKTGELKAPIVIGRDHLDSGSVA
                     SPNRETEAMQDGTDAVSDWPLLNALLNCASGATWVSIHHGGGVGMGFSQHAGMVIVAD
                     GTDAAARRLERTLWNDPATGVMRHADAGYEDAIKCAKEQGLNLPSLS"
     gene            21456..21941
                     /locus_tag="P5558_06055"
     CDS             21456..21941
                     /locus_tag="P5558_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HRY23905.1"
                     /translation="MSADLDDLYERDFYAWTRRQAAALRHAGVERINTQEPIDWANLA
                     EEVEDLGNEQRRAIASQLERLIQHLLKLEFAEDRTPRRKWLLSIDNARAEIERRWTAS
                     IAREIEPTLPRAHARARRAAALELEDHGEPKTAQALPEGPHYTLEQLLDESWYPTAEE
                     R"
     gene            complement(21978..22478)
                     /locus_tag="P5558_06060"
                     /pseudo
     CDS             complement(21978..22478)
                     /locus_tag="P5558_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3320 a         8856 c         7074 g         3293 t
ORIGIN      
        1 tgctcctcga ccatccggca cggccggccc tcgtcgccgc cgataccgtc tccgccgcca
       61 aggcaatgcg gctcgccgat catctggccc ggctcgacct gctcttcacc aatgccgccg
      121 aggcggccgc cctcgtcggc cccggggcgc cggcggagct ggcccggcgc ctcgtggcga
      181 aaggcgtggg cgcggtcgtc ctggggcagg gggccgccgg cctcgccctc gccgaccaca
      241 acggcgtcca cgcgctcgat gcccacaccg tgcccgtcgt cgatgtcacc ggggccggcg
      301 acgctttggc cgcggccgtg ctcgccgccc gcctcgccgg gctcgacctc gccgccgccg
      361 gccgcatcgg ccgcctcgcc agcgccctcg cggttgcctc cgacgaggcc gtgccgaagg
      421 atttcggcat cccggccttg cgcgacctcg cccttcggct cgatagtgag gcccatgtcc
      481 agcttgctcg cctttagccc cgaggtcgcc gccgcccgca aggccggcca gccgatcgtc
      541 gccctcgaga gcacgatcat cgcgcacggc ctgccgcggc cccgcaacct cgagaccgcc
      601 cgcctgctcg aggccgaggt gcgcgaagcg ggtgccgtcc ccgccaccat cgccgtcgcc
      661 gacgggcgcc tcaggatcgg cctcgacgca gcccagctcg aggagctcgc cgttgccgac
      721 ggcgtgctca aggtctcgag gcgggacctc gccggctgca tcgccaccgg ccgcaccggg
      781 gccaccaccg tcgccgccac catggtcgcc gcccagctcg ccgggatccg ggccttcgcc
      841 acgggcggca tcggcggcgt gcatcgcggt gccgagcgcg acttcgacgt ctccgccgac
      901 ctcgaggagc tggcccgcac cccggtcgcc gtggtctgcg ccggggcgaa gtccatcctc
      961 gacctgccga agacgctgga ggtgctggag accaagggtg tggccgtggt cggctaccgc
     1021 accgacgagt tcccggcctt cttctgccgg gccagcggcc tcgccgtgcc cctgcgcctg
     1081 gattcggtcg ccgagatcgc caacctcctg gacgtgcagc ggcggctcgg cctgaccagc
     1141 ggcgtgctcg tcgtcaaccc gatcccgaag gccgccgccc tgcccgagga gctggtcgag
     1201 ggggccatcg ccgaggcctt ggccgtcgcc accgccgccg gcatcggcgg caagcagatc
     1261 acgcccttcc tcctcgcccg gatcgtcgag ctcaccggcg gccagagcgt cgaggccaac
     1321 gtggccctcg ccctgagcaa cgtgcgcctc gccgccgaga tcaccgtggc cctccacgca
     1381 ttgggcgagc cgatcctgcc gaccccccgc atccgcccgc gcagccgtca gatggacgcc
     1441 atgtagccgc cgtcgacata gaggatctgg ccggtgacgt agcgtgccgc gtccgaggcg
     1501 aggaagacgg cactgccgac gagatcgcgc agatcgccga accgccccgc cgggatcttc
     1561 gccagcatgg cccgctgcca ggcctcgtcc gcatagaaca tctcggtcag cgctgtgcgg
     1621 aaatagccgg ggccgatggc gttcaccctg atcccgagcg gcgcccactc cgtcgccagc
     1681 gtccgggtca ggccgagcag cccggccttc gaggcgccgt agggtgcggc cgtcggcacg
     1741 cccaccgccg aggtgagcga gcagatgttg aggatggtgc cgccctccgc cgcgcccatc
     1801 agccgggccg ccgcctgggc ggtgaagaag gcgcccttca ggttggtccc gtggatccgg
     1861 tcccagagac cctcctcgac ctcgagcgag gcgcagacct cctcggtgcc ggcgttgacg
     1921 accacgaggt cgagcccacc cagcgctgcc gccgcctcgg ccacgccgcg ccttatgccg
     1981 tcgaccgaac gtgcctccat ggcgaccggc accgcctttc gcccctgctc ctcgatcgcc
     2041 gccaccacgt cggcgagccc ggccacgtcg cgcgccgtca ccgccacgtc cgccccgaac
     2101 cgggcgagcc cgatggccag cgcctcgccg atcccccggc tggccccggt gacgagggca
     2161 cggcggccct tcaggtcgaa actggcgtcg acggcgtcga tcatggcggc actccggtcc
     2221 ggcagggagc tggggtcagg cggcagaggc tgcacgcacg ccggcccgcc cgcaaggtgc
     2281 ggcgtgtcgc ctgaggcgat ctcgccccct tgtcgggcgg cctctctccc ggcaccctgc
     2341 cgacggtttt cccactccgg cagaggtgac cgcgtgcacc ccatggtgcg gctttcaggc
     2401 gtcggcaaga cgttcggcgg gacggtggcg gtcgacgccg tgtcgctcga cctcgcggcc
     2461 ggccgcttcc tcaccatcct cggcccctcg ggctgcggca agaccacgct gctgcgcatg
     2521 atcgcgggct tcatcgcccc caccaccggg cgcatcgagg tcaacggcgc cgccgtcgag
     2581 agtgtcccgc cctggcgccg ctcgatcggc atggtgttcc agaggctcgc cctcttcccg
     2641 cacctctccg ccttcgacaa cgtcgcctac ccgctccgca tgcggcgctt tccggccgcc
     2701 ggcatcgccg agcgggtcga gcgctatctc gagctggtcc gcctgcccgg catgggcggg
     2761 cggcggccgc acgagctttc cggcggccag cagcagcgcg tcgccatcgc ccgcgccctc
     2821 gtcttcgagc cggacatcct cctcctcgac gagccgctcg cggccctcga ccgcaagctc
     2881 cgggaggaca tgcagctcga gttccgccgc atccagcagg agctgggtgt gaccaccatc
     2941 aacgtcaccc acgaccagcg cgaggcgctc gtcatgtcgg acgaggtgat ggtcatgcat
     3001 cagggccggg tccagcagtc ggcgccgccg gccgagatct accgccagcc ggccaaccgc
     3061 ttcgtcgccg gctttctcgg cctcaccaac ttcctcgacg ccgaggtcct gcgcgccgag
     3121 ggcgagcgcc gggtcgtgcg gataggagga gcggagctga gcggcaccgg caatgtcgcc
     3181 accggtgccc gggtcacggc ggccctccgc gccgagaagc tccgcctgct cgcccccggt
     3241 gccgtcgccg agacgcagtt cccggccacc gtcaccgagg tcgtgttcga gggcgaccgc
     3301 atcctctatg aggtccgggt cgacgggctc ggcaatgccc ggctccagct cttcgaccac
     3361 gaccccgaag gccacggccg gcacagcccg ggtgatatcg tgacgatcgg ctggtcgtcc
     3421 gcccatcttc tgcttttcac cgactgaacg acacaacgac ggaagggaac cctcgatgac
     3481 gaagccgctc accaccatcg gccgccgccg cctgctccag gcggccggcc tcggcaccac
     3541 cgccctcgcc acgccctggc tctggcgccg gccggccatg gcccaggaga agcccgccga
     3601 gctcatcgtc cgtgcctggg gcggcgtctg ggtcgaggcg ctcgacaagg gtgtctcgca
     3661 gcccttcacc gaggccaccg gcatcgccat ccgccacgac ctcaccgagg acaacgagat
     3721 ccgcccgcgc atctgggccg ccgtcgacca gggccgcgtg ccgccgatcc acgtcaactg
     3781 ggacacctcg accaacgcca ccaagtcgtc gctccggggc gtcaccgagg atctgggcga
     3841 tcttccgaac ctcgccgaca tgctgcccgc ggccacgccc gccggcttcg acggcgtgcc
     3901 gatgatcaac atgtactcct atgtctacgt cctcgcctat cgcgacgagg ccttccccga
     3961 tggcccgccc gtgagctggc gggaaatgct ggagccgcgc ttcaagggcc gggtcgccct
     4021 ctacaacgac ggcatcggct tccacccggc ggcgcagatc gccggcggcg gcacgctcga
     4081 gggcattccc gacgacatgg agccctgctg ggcgttcatc cgcgagctca gtgcgcagga
     4141 gccgctgctc ggcgaggacc cggacttcac cgcctggttc cagaacggcg agatcgacat
     4201 cgcctgcacc atcctctcca acgcccgggc cgccaagcag aacggcatcg ccgtctcctg
     4261 gaccgtgccg gaggagggcg ccaaggtcga caccgacggc atgtggatcc ccaagggcct
     4321 gcccgggaac gagctccatt gggcgaagga atacgtcaac ttcgccatga gccaggccgc
     4381 ccagcaggaa tggacgagcc tgctcggcct gcccggcctc cgccccggcc tcaccgcccc
     4441 cgccgacctc gccggcgatc ccgcctaccc gcaaaccccc gaggacttcg cccgcctcat
     4501 ctccgtcccg acccggatcc tggtcgagaa cgagagcgac tggttccaga agttccagga
     4561 gatcatgcag ggataggccg gatcatgggg gaggcgtggc ctcccccatg cgccccctcg
     4621 ggacttgcga ttggccacct cggcgactag attagccaag gttagccaag gagcccccgc
     4681 cgtgcacacc gtgaacatgt tggaagcgaa gacgcagctc tcgaagctcg tcgaggccgt
     4741 cgagagcggc gccgaagcgg agatcgtcat cgcccgtaac ggcaagccgg cggcgaagct
     4801 ggtgccgatc ggcgtggaga agacgaaaag gcggccgatc ggcttcctcg acggcaagta
     4861 tccctccacg agtcaggaag agttcgattc caccaacgat gaaatctata aaatgttctt
     4921 cggcgaagag gagtgaagct tctccttgat actcaaatag cgatctggtc gatctctggc
     4981 tcagagaggc tgagcgggca gatgcgtgag atgctggcgg ccgggagcaa cgatatcttc
     5041 gtctcgtccg tcagcatttg ggaaatagcg atcaaattcc cactacgaaa gccatcagct
     5101 ccgcccttct cggccagtga ggcagtccgg cactgccgca ccatcggtta caccctcctc
     5161 tcaatcacac ccgagcacgc cgcggccgtc ggcacgctgc cgcatcatca cggcgatccc
     5221 ttcgaccgcc tcctgatcgc ccaggcgctg accgagccgc tgcggctcgt caccgccgac
     5281 cgcaggctcg ccgcctacag cgacaccgtc atcccgtgct agcccagctc gcccttcgtc
     5341 cggatcacct tgtgcaagag gccgtaggcc atggcctcct cggggaacat ccagtggtcg
     5401 cggtcggtgt ccttggcgat ccgctcgatc ggctggccgg tctcgttggc gaagatgcgg
     5461 ttcagccttt cgcgcatctt gatgatctgc tcggcctgga tgccgatatc cgacgccgac
     5521 cccatcatcc cgcccgacgg ctggtgcagc aaaaagcgcg tgttcggcag gcagaagcgg
     5581 ttctccctcg ccgcgccgac gaagatcagc gcacccgccg aggcgaccca gcccgtgccg
     5641 atcgtccgga ccctggggcc cacgaacttc agcatgtcgt agatcatgtc gccggattcg
     5701 acgtgcccgc cgggcgagct gacgaagacg ttgatccagt cgtcgccgtc gtcggccagc
     5761 gccaggagct gggccgccac cagcttcgcc atcttgtgct cgacctggcc ggcgatcagg
     5821 atggtgcggc tcttgaacag ccgctcctcg agcatgccgc ccgccttggc gccatcgtcg
     5881 ggcttcttct tctcgtcctc gtcgtccagc cacatcgaat ggaatctccc ttggcggcca
     5941 gccacccgca gttgagcgcc ggcctttgaa gcctcgtggc accgtaccta gcgccccgcc
     6001 ggccgaagaa caatccgcgc ctgcccgcac gctcggcctg cgcctgctcg acggcctgga
     6061 aggggtgcgt gcgaccctct tccccagggg cgccggcggc ctcaccccct ggctcttcct
     6121 cctcccggcg ctgctcctca ccttcctcct cgtcgccggc atcgtgccct tgctcgacac
     6181 ctcgctgcgc cagctcgacc gcgccacctt ccgtcccggc gaggactgga cgctcgccaa
     6241 ttttcacacc ctctgggaac gtcccgtcta cacttggctc gcggcccgga cgatgggggc
     6301 cgccctcgtc gtcaccgtcc tcagcctcct gctcgccctg ccttacgcct ggctcatggt
     6361 ccgcacgccc cggccctggc tgaagaagct cctgctcgtc tcgctcttcc tgcccttctt
     6421 catcggccag gtggtgcgcg cctatggctg gctcgtggtg ctgggcaacc agggcctcgc
     6481 caatgccgtg ctcggctggc tcggcctgcc accggtgacg atcctctaca ccctgccggg
     6541 cgtcgtgctc ggcctcgtcc agtacatgct gccgtttgcc gtgctgatgc tggccccggc
     6601 cttcaccgcg gtgcccgagg agatcgagct cgcctccggc agcctcggcg ccagcccggc
     6661 gcgcaccttc cgccacatcg tcctgcccct ggtccgcccg ggcctcgtcg ccgccgcggt
     6721 cgtcgtcttc accctcacgc tcaccgactt cgccatgccg gcgatcatgg gcggcggccg
     6781 caacgacttc atcgccaacg ccatctacga tgccttcttc tcgctcagcg atccgggcct
     6841 cggcagcgcc ctcgccatcc tcctgaccct cgccggcacc accatcgccg gcatcatgct
     6901 cgccctcttc gggctcggca cgctcggcta tgtgcggcgc ggctgatgca gcgctcgccc
     6961 ttcaccctgc tgctcggcct ggtcgtcgcc ttcgacctcg tcttcctcgc cgtgccgacg
     7021 ctggtcgtcg tcgtcatctc gttcaccagc ggcaacatgg tggccttccc gcccgagggt
     7081 ttctcgctcc gctggtacgg tgtgctgctc accaggcccg agttcctcgc cgccctcggc
     7141 cgctcgctgc aggtcggcct cgtctgcaca gcactggcga tcccggccgg cacgctcgcc
     7201 gcgctcgccc tcgcgcgctg gcgcatccgc tatcccacgg gccttcagct ctatctcctg
     7261 ctgccgttca ccgtgccatt ggtcgtctcc ggcatcgcgc tcatgctgct cttcggtcgc
     7321 atgggcatgc tggggcaggt ctggccggtc gggctcgccg cctgcgtcat caacctgccc
     7381 ttcatgatct gggcggtggc ggcgtcggtg aaccacctcg accccgacct cgagaacgcc
     7441 gccatgaact gcggcgcacc gcccacccac accttcttca ccgtcaccct gccggccgtg
     7501 gcacccggca tcctgaccgg ggcgctggtc atgttcatcc tcgccgtcaa cgagttcctg
     7561 gtcagcctga tgctggtcga cgcgcgcatc gtcaccttgc cggtcctcgt ctacaacgcc
     7621 atccgcaaca tcatcacgcc cgacctcgcc gccgtctcgg tcgtctacct gctgatcgcc
     7681 gtgatcgcga tctggctgct cgaccgcctc gtcggcctcg gcatcctcct cgagtccaag
     7741 cggagctgac gcagcccatg atcaatctct tcgagctcga ccagctctcc accgccgagc
     7801 gcgaggccct cctgctgcgc accgaggccg acctctccgc cttcatgcag cccgtggccc
     7861 ggatcgtcga ccgcgtgcgc gacggcggcg acgctgccat cgccgagctc gcccgcgaga
     7921 tcgacggtgt caccctcgac cccgccgggc tcgaggcgac cgaggccgag ttcgccgccg
     7981 ccgacgcggc gctcgaccag gacgtcaagg acgccatcgc ctacgccacg gacaacatcc
     8041 gccgcttcca cgagcgccag aagcccgagg acatgtggct gatggagatc cagaagggcg
     8101 ctttcgccgg cgagcgctgg tcgccgatcc gctcggtcgc ctgctacgtg ccgcgcggca
     8161 agggcgcctt cccctcggtc atgatgatga ccaccatccc ggccgtcgtc gcgggcgtgg
     8221 ccgagatcat cgtcctcacc ccgccgaccc cggagggcgg catcgacgcc gcctcgctcc
     8281 acgccgcccg catggccggc gtgcgccgcg tctttcgcgt cggcggggcc gtcgccgtgg
     8341 cggctgccgc ctacggcacg gccaccatcc cgaaggtggc gaagatcgtc ggtcccggca
     8401 gcccctgggt ggtcgccgcc aagcggcatc tggcggaccg gatcgatacc ggcctgccgg
     8461 ccggcccgtc cgaatccatc gtcctcgccg acgccacggc caaccccgac atcgccgccc
     8521 tcgacctcct gatcgaggcc gagcacggcg ccgacagcag cgcctatctc gtcaccgaca
     8581 gccgtgaggt cgccgagcgc gcccgcgccc gcctgcccga gctgatcgcc aatctgggcg
     8641 cggagcgccg gggctacatc gaggccgtcc tcggcggcaa gtgcggcggc atcgtcctcg
     8701 ccccctcatg ggacgaagcg gtccgcttca ccaacgacta cgcgcccgag cacctcgaga
     8761 tcctctcgaa ggagcccatg cagcacctcg gtgcgatcac cgatgccggc gagatcctgc
     8821 tcggcgagaa cacgcccatg accatcgcca atttctgcct cggccccgac tgcgtcctgc
     8881 ccaccggcgg ctgggcccgc accttctccg cgctcggcgt gcaggacttc atgaagcgca
     8941 ccagcctcgg ccacgtcacc cgcgccggct tcgccgagcc ggcccgtcac gcggcggtgc
     9001 tggcccgcta cgagggcttc gaggcgcacg cccaggcgct gggtcccatc cgcgacacgt
     9061 tcaggccgta ggaatcctcg accccggacg tttcgtcact tccgccagac cccgccaccg
     9121 tccagactgg agcggttgac taggctcccg ggaagcgatg cggcaggcgc ggcgatatgg
     9181 cgggcgatgg gcgggcaggg cggcgctcgt ggcgctgctg ctgcagctcg cccagccgtg
     9241 gctcggcctg cagccggtca gggcggaggt cgggcccacg gggcctgtcc tcgtcatctg
     9301 caccggcagc ggcctgctcc gcatctacgc cgacggcacg ccggtgcagg agaccgaccg
     9361 gcagggcctg cattgcacgg cctgtgtcgc ccggctgcac ggtccggcgc tgctgccgac
     9421 cgccccggcc ctgcgcctgc cgagggtcgc aacgcccgtc gccacccgcg tgtcggcgcc
     9481 cggaaccgct gccagagcca cggccccgcc actgccggcg cgcggcccgc caacggtctg
     9541 agccacgctc aagaattcct ttcccgtcaa cgacacgggg cgtctcgcgt ggcctttggc
     9601 ccgccgcccc tgcatctcac tctggagacc atccatgctg cgctggattc tttcgggcac
     9661 ccttgccctt gccgccatcg gtaccgttca ggcgaacgag ctcacggtcg gcgagatcac
     9721 catcgatcac ccctgggccc gggccacggc ggcccaacag gcgaacggcg cggcctatct
     9781 cgtagtcgac aacggcggcg aggcggaccg catcctgggt gctgcgagcc cggtcgcagg
     9841 ccgggtcgag ctgcacaacc acatcgacga caacggtgtg atgcgcatgc gccgggtcga
     9901 ggccatcgac ctccccgccg gcgagaccac cgcgctcgcc ccgggcggcc tgcacatcat
     9961 gctgttcgag ctcggcgcgc ccctggtcgt gggcgagacc ttcccgctca cgctgaccct
    10021 cgagcaggcc ggcgcggtcg aggtcgaggt cacggtcgag ccggtgagct acggcatcgg
    10081 gggcgacgcc aagggccatc agggccacgg cagcggcggc tgagacaagg ccgggcagca
    10141 cctgcttcag gcaggtgctg cccgcaagct cactcggccc gcttcatgcc ggtgaccatg
    10201 gcgcgcacca ggttctcgcc atgcgagagg ctcgagaaga tcacccccgc gatgtgcgtg
    10261 gcgatcagca ccagcatgag gttggccgcc acctcgtggg tctcctcgag ccatctgggc
    10321 tcgtggccga ggaggtcggt cgtgagcagc cagcccgagc cgccggtcac cacgagcccg
    10381 gcgagcagcg cgaggaccat ggccgcaccc aggggattgt ggccgagata gcgcttcgcg
    10441 cgcatcgcca gcgcgtcccg cgcataggcg agggtggtcg cccggccgaa ggcgaagtcc
    10501 cgaaaccgcg catgcggcga gccgatgaag ccccagacga cgcgcatgcc gaccagcgcc
    10561 aggacgccgt agcccaagag ctcgtgcagc cagtcgatct cctcgcccgt cagccacgcg
    10621 gcggtgaaga agaccacgag cgcccagtgg aagagccgga cgaacggatc ccagacccgc
    10681 accagccgga cgtccgcgtc cgctccggcc ggtgcggcgg cggccatcag tcgtcctcgc
    10741 ccggcacgac cgcgcccgag accggatcga cataggcctc gacctcgaag ccgttggcat
    10801 cgcggccctc gacctcgtag caattgccct cgcggtcgat ctcgcgcacc tcgtagccca
    10861 tgccctggag cttggcgcgg agcgcatcct cggtcatcca ctgggcacgc gggacgtcgg
    10921 cgcagtcggg gccggaggcc agggccggca gggccagcgc catcggcacg agggcggtgg
    10981 aaagcatcag gagcttgcgc atgttcgagt tctccaattc gtcagccggc aggatcgccg
    11041 acgccgcgga ccataggctc gccctgctgt cgccggcctg tcggtccttg tcagccttct
    11101 gtcagcgctc gctgcctatg ctcgccgcat gacccgcacc cgcctcgccc ttctcgcttc
    11161 gctcctcgct tcgctgccgt tggccaaggc tgctgccgac agcgaccacg agcgcgcgcg
    11221 acaggcgctc gagcggggcg aggtgctgcc cttggccgag atcctggagc gcgtgcgcag
    11281 ccaggcgccg ggcgaggtga tcgagaccga gttcgaccgt gagggcgggc gctgggtcta
    11341 cgagctcaag ctgatcgatc cgcagggccg cgtggtcgag ctcgaggtgg acgccgtgga
    11401 cggccgcatc ctgcgctcgg agctggacga ttgaggctgc tcctggtcga ggacgaccgc
    11461 cgcatcgccc gggacgtcat cgccctgctc gaggcccagg gccacctcgt cgaccacgcc
    11521 accgacggcg aggatgcctg gttcaagggc gacgtcgagg actacgccgc cgtcgtcctc
    11581 gatctcggcc tgcccggcat ggacgggctc gccgtgctca agcgctggcg cgagaacggc
    11641 cggaccatgc cggtcctcgt gctctcggcc cggggcacct ggaaggagcg cgttgaaggc
    11701 atcgacgcag gcgccgacga ctacctgccc aagcccttcg agccggagga gctggccgcc
    11761 cgcctccgcg ccctgatccg ccgttccgcc gggcagggcc ggccggtgct cgagatcggc
    11821 gatgtcgccc tcgacccgcg cgccatgcgg gtcagcgtca agggcgtgcc cagggcgctc
    11881 tcgccgcagg aattccgcct gctcgactac ctgatgctgc aggccggccg ggtggtgccg
    11941 ccgagcgagc tgctcgagca gctctatggc gacgaggaca gccgcgagac caacacgatc
    12001 gaggtgctgg tcggccgcct gcgcaaaaag ctcggcgtcg agctgatctc gacccggcgc
    12061 ggcttcggct acacgatcga gcgggacgac ccggcacctt gagcagccag tccctgcgcc
    12121 tgcgtctgct gctggcccag gccgcgtcgg tggtcgtggc cctcgtcctc gccggcatcg
    12181 gcctcaccgt gctcttcgag cgccacctcg agcgccgcgt cgaccaggag ctcgcgacct
    12241 atgtgcgcca gctcgcggca cacctcgtca tcgatgcgga tggtgctctc aacctcgagg
    12301 caccgctcgc cgacccgcgc ttcgccgtcc cgctcagtgg cctctactgg cagatcgagg
    12361 atcaggggac cggcaccgtg ctccgctcgc gctcgctctg ggacgacgtg ctgccgctgc
    12421 ctgtggacga gcccgagccc ggccaggccc accgtcaccc gctgcccggc cccggcggca
    12481 gcttgctcgt cgtgcacgag cgcctggtgc tgctcggcaa tggtccaggc gagcggcgca
    12541 tccggattgc cgcggccgtg gaccgcgcca cgctcgatgc cgcaggcacc gactatgccc
    12601 gccagctcgc catcgccctt gccggcctcg ctctggtgct ggtcgctgcc gccgccgtgc
    12661 aggtctggta cggcctgcgc cccttcgagg cggtgcgccg ggcggtgacg gcggtgcgcg
    12721 agggccgggc gaagcgcctc gacggccgct tcccggacga gatccgccca ctcgtccagg
    12781 aggtcgacgc cctgctcgaa gcgcaggagg gatcgctggc ccgggctcgc cagcgcgccg
    12841 ccgacctggc gcacggcctg aagacgccgc tcaccgtgct cggcagcgag gcccgcggtc
    12901 tcgccgccag gggcgagacc aaggccgccg ccgagctcga ccggctggcc cgggacatgc
    12961 ggcgtcacat cgagcgcgag ctcgcccgca cccgcctcgc cggcgccacc agccgggggc
    13021 aggcgacggc gctcgccccg gccatcgacc ggctggtgcg aaccctccgc cgcacgcccg
    13081 caggggaggc gcgggactgg cggctcgatg tgccggcgca cctcctggtc gcggtcgagg
    13141 ccgacgatct gggcgagctt ctgggcaacc tgctcgacaa cgccgccaaa tgggcaaggc
    13201 agtcggtgac cgtccaggcc gtggcggacg ccggcgacgt gcggctcgcc atcgccgatg
    13261 acgggccggg cgtcgatgcg gccgagctcg accagctcgg cacgcgcggc catcggctcg
    13321 acacgggaaa gcccggccac ggcatcggcc tcgcgatctc ccgggacatc gcgctcgcct
    13381 atggcggaac catcgccttc gccaaccatc cggatggcgg gcttcgggtc agcctgaccc
    13441 tgccggccgc tcagctcccc actggccagg cctccatgcg ataggcgctg tcccagaaga
    13501 gccactcgta ttgcgccgag cggatgaagg ccgccgccat ccgttcacgc tcgtcggccc
    13561 cggcccgctc ggcggccata tccgtgatag cgatcacctc ctccaccgac cgggcgaagt
    13621 ccggcgaggc ataggtgtcg atccagggct ggtagaagtt gcccgccgcc gacccctgcg
    13681 cgatccgcgt gccgacatcg tgatagatcc aaaagcacgg caaaatcgcc gccaccagca
    13741 ccggccagga ttcctggtgc gccgtggcca ggaggaagtt cgtgtaggcg aggcaggaag
    13801 gcgagggcag ggcagccgcc agctcggcag cgctcacccc gaaccgctcc aggaacgtcc
    13861 cgtgcagcgc ccgctcgacc ttgagcgccc ccatggccga gcccgcgaac ttctccgtcg
    13921 ccgccagatc gggcgccttg ccggccgcca gcgtcagggc ccgcgcgtac tggtcgagat
    13981 agagcttgtc ctgcagcatg tagaacctga accgctcgcg gctcagggtc cccgccgcca
    14041 gctcgcggtt gaacggcaac gcatcgatcg cccgccgcag ctcccccgtg cgtgtccaga
    14101 tgtcgtcggt aaacgccatc gcctcctcct tcccgaaatt cgatctactt ctgctgccag
    14161 gcccacgtcc tcgcacagga ccagacccca tgaccttctt cggcgccacg ctacgcggca
    14221 cgctcgccct cggccttttc ctcgccgccg gatcggccgc ggccgagacc atcggcgacg
    14281 tctcgaccaa gttccactgg atctcgcccg accacaagat cgtcgtcgag gccttcgacg
    14341 acccgaagat cgagggcatc agctgctacc tgagccgggc caagacaggc ggcttcaagg
    14401 gtgccttcgg ggtcgcggag gacacgtcgg atgcgtccat cacctgcctg caggtcgggc
    14461 cgatcaccgt caaggacgag ctggacgagg gcgaggaggt cttcaagcga cgcacctcgc
    14521 tgatcttcaa gtccatgcag gtggtgcgct tcctcgacga gaagcgatcg accttcgtct
    14581 acctcgtcta ttccgaccgg gtgatcgagg gcagccccaa gaactccatc tcggccgtcc
    14641 cggccatgcc ctggggcaat gtgcagcccg atctggcgaa ggcccgctga ccggcgcgtt
    14701 caggcatcgg ccagcaggcc ccgcatcgtc cgttcgaacc gccgggccac ggcctcgccc
    14761 gccacatgcc ggcccccctc gaccacgagc cggccgccga cccggacggt atcgaccggc
    14821 gccgtcctcg cggcgaagat ccagccgtcg agcagcgcgt cgccgctgcg cccgaccagc
    14881 gccggatggc cggcgtccag cgtcacgatg tcggcccgcc gcccgaccgc gatcgccccc
    14941 gtgttctgcg ccagcgcctg agccccgccg gcgagcgcct tctcgaggag cgtccgtccg
    15001 accgaatggc cgggggccgc cagccggttc cgccgccggt cgcgcaggcg ctggctgtat
    15061 tcgaggctcc gaagctcgtc cgcgagatcg atgcggatat ggctgtcgga gcccacaccg
    15121 aagcgcccgc cccgaccgag atagtcgaca ccgcgaaaga taccgtcgcc gagattggcc
    15181 tcggtcaccg ggcacaagcc cgccaccgcc cccgaggccg cgaggtcccg cacctcgccg
    15241 tcgtccagat gcgtgcaatg aatgaggcac cagcgcccgt cgagccccac ctcgtcgagc
    15301 agccagcgca ccggccgccg ccccgaccag gcgaggcagt ccagaacctc cttctcctgc
    15361 tccgccgcgt ggatgtgcaa cggcccagcc gtgcaggcct cgaccaggcg cctgaggctc
    15421 tccggcgtca ccgcgcgcag gctgtgcggc gccgcaccca cgaccagatc cggatgacgc
    15481 gcgcgcagcg tccggaccag ctcgagcaga tcctcgacat ccatgacgaa gcgccgctgg
    15541 cccggcgccg gcggtgccgc gccgaagctg ccctggtggt agagcaccgg caaatgggtc
    15601 agcgccatgc ccgtcgccgc cgccgccgcc acgatccgct cggccagctc ggccggatcg
    15661 gcgtaaggcc ggccatccgt gtcgttgtgc agatagtgga actcgccgac cgcagtgaag
    15721 cctgcctcca gcatctcggc atagagctgg ccggcgatcg cctgcacgtc gtccggggtc
    15781 agcctgtcga ggaagccgta catcacctcg cgccaggtcc agaagctgtc ctccgccggc
    15841 ccggcccgct cggcgagccc cgccatgccg cgctggaagg cgtggctgtg gacgttcgcc
    15901 atgcccggca cggcgatggc ggcgcgcgcc gcatccggcg ccggcaccgc atttggctcg
    15961 accgaatgga atgtcccgtc cggtgcgacc tcgaccgcca cgtcccgggc ccagccttcg
    16021 gccaagagcg cctgcgcaaa aaagagcttg atcatcctgc cctcccgtcc aaccctgctt
    16081 ctgaccaaat cccggaaagg cggcaagcgc ttggcgccaa catccagaat gctcctgacc
    16141 aacgctcgct tcgccacgat gcaatcgggc ggtgctccct atggcgccct cgatgatgcg
    16201 gccctcctga tcgaggccgg ccgcatcgcc tggctcgggc cgctgagcgc ggcgccgagg
    16261 gcggccgaga cggccgatct cggcggccgc tgggtgctgc ccggcctcgt cgactgccac
    16321 acccacatcg tcttcggcgg caaccgggcg cgcgagttcg agctccggct cgaaggtgcg
    16381 acctacgagg aggtggcccg ggccggcggc ggcatcctct cgaccgtcaa ggccacccgc
    16441 gccgccagcg tcgacgagct cgtcgcccag gctttgccgc gcctcgacca tctcctggcc
    16501 gaaggtgtga ccacgatcga gatcaagtcc ggctacggcc tcgagctcga gagcgagatc
    16561 cgcatgctcg aggcggcccg ccggctcgcc gacgagcgcg acgtcgaggt ccagacctcg
    16621 ctcctcgccg cccacgccgt cccgcccgag ttcaagggcc gcgccgatgc ctatgtcgac
    16681 atcgtcgtgt cccagatcat ccccgccgtg cgcaaggccg gcctcgccga cgcggtggac
    16741 ggcttctgcg agggcatcgc cttctcgccc gcccagatgc gccgcgtctt cgagaaggcg
    16801 agggcggtcg acctcccggt caagctgcac gccgagcagc tctccgacct caagggtgcg
    16861 gtgctcgccg ccgggttcgg cgccctctcc gctgaccacc tcgaatatgt ccaggaggac
    16921 ggggtgcgcg ccatggcggc cgccggcacg gtcgccgtgc tcctgcccgg cgccttctac
    16981 acgctccggg agaagcaggc gccgcccgtc gacctcttgc gccgccacgg tgtccccatc
    17041 gccatcgcca ccgactgcaa ccccggctcc agccccgtga cctcgctcct cctcgtgctc
    17101 aacatggccg ccacgctctt tcgcctgacc cccgaggagg ccctcgccgg cgtcaccagg
    17161 aacgccgccc gcgccctcgg cctggaggcc gagatcggca cgctcgaggt cggcaagcgc
    17221 gccgatctcg ccatcttcga catcgaccac ccggccgagc tcgcctaccg catcggctac
    17281 aacccgctct tcgccagcct gcgcgagggg cgctggcgtc catgctgacg ctccgcccgg
    17341 gtgccgtcac cctcgccgaa tggcgcgccg tgctggcagg cacccccgtc accctcgacc
    17401 cggcctgccg ctccgccgtc cgcgcttccg ccgaagccat ccgcaccatc gtcgccgaga
    17461 accgcaccgt ctatggcgtc aataccggct tcggcaagct cgcctcgacc cgcatcgcca
    17521 agggcgacct cgagctcctg cagaagaacc tcgtcctctc gcactcgacc ggcaccggcc
    17581 cgccactgcc cgacccgctc gtccgcctga tcatggcgat gaaggcggcg agcctggcgc
    17641 gcggctattc cggcatcagc gaaggcaccc tcgaccagct cctggccctg ctcgccaagg
    17701 gtgtcctgcc cgtcatcccc gagaagggct ccgtcggcgc ctcgggcgac ctcgccccgc
    17761 tcgcccacct gaccgccgcc ctcctcggcg tcggcgaggc ccgcctccat ggcaccaccc
    17821 tgccggccgg caaggcgctc gccgaggccg gcctcgcccc ggtcaccctc ggccccaagg
    17881 agggcctggc gctgctcaac ggcacccagg tctcgacggc gctcgccctc gcggccctcc
    17941 tgcgcatcga gaccctcttc aggaccgccc tcgtcaccgg ggcgatgagc gtcgacgccg
    18001 cccgcggctc ggacgccccc ttcgacgccc gcatccacgc cattcgcggc cacccgggcc
    18061 agatcgccgt cgccgccgcc ttccgcgcct tcctgcacga gagcccgatc cgcgccagcc
    18121 acgtcgactg cggcaaggtc caggacccgt actcgctccg ctgccagccg caggtcatgg
    18181 gtgccgtgct cgaccagctc cgcatggcgg ccggcgtcct cacgatcgag gccaacgccg
    18241 tgagcgacaa cccgctggtc ttcgccgaga gcggcgacgt cctctccggc ggcaacttcc
    18301 acgccgagcc cgtggccctc gccgccgacc agatcgccat cgccatggcc gagatcgccg
    18361 ccctcgccga gcgccgcatc gccatgctca ccgacggctc gatctccggc ctgccgccct
    18421 tcctcgtcgc cgaccccggc ctcaactcgg gcttcatgat cgcccacgtc accgccgccg
    18481 ccctggcatc cgagatcaag cagcgcaccc acccggcatc cgtcgacagc ctgcccacct
    18541 ccgccaacca ggaggaccac gtctccatgg cgacccacgc cgcccgccgc ctccacgcca
    18601 tggccgacga cctggaaggc atcatcgcca tcgagctcct ggcggccggg caggggctcg
    18661 acgtccatct gccgctcacc acctcgagcc gcctcctcgc cccgcaccgc acgctccgcg
    18721 cccatgtcgc cacctgggac aaggaccgct tcttcgcccc cgacatcgaa gccgcccgcc
    18781 gcctcgtcgc cgagggtgcg gtcatcaagg acagcctcga catcctgccc gggctcgaaa
    18841 cctcatgata acggtcgacc catgatcaca gtcgacgtcg aggagcgcgg cacccccctg
    18901 ttgctcagcc tgccgcacac cggaaccggc ctgcccaagg gcctcgccga gcgcctgacc
    18961 gaggccggcc gcgccctcgt cgacacggac tggcatgtcg accgcctcta cgccttcgcc
    19021 gacgccacag gcgccaccac cgtcaaggcc ggcttctccc gctacgtcat cgacgtcaac
    19081 cgcgacccct ccggccaatc gctctacccg ggccaggcca cgaccgggct ctgccccacc
    19141 accaccttcg acggcgagcc cctctacctt tcgggcgagg agcccgacgc cgccgagatc
    19201 gaggaccgcc gccagcgctg gttcgaagcc taccaccagg ccctcgccgc ccagatcgcc
    19261 cggctgaagg agcgcttcgg caccgtccgc ctctacgact gccactcgat ccgctcggtc
    19321 gtcccccgcc tcttcccggg caccctcccg gtcctcaatc tcggcaccaa cgaaggccgc
    19381 tcctgctcgc ccgccctgcg cgcgcagctc gtcgccgtca tggccgaaag cggcctcacc
    19441 cacgtcgccg acggccgctt caagggcggc tggatcaccc gccactacgg cgatccctcg
    19501 aacggcatcg aggccgtgca gatggagctc gcccagtccg cctacatgga cgaggcgccc
    19561 ccctggccgt atcgcgccga cctcgcggca agactcgagc cgaccctccg ccgcctgctc
    19621 gagacgctgc tcgcggccaa aggagaaaaa acatgaaccc gcgcctcgac aatgcccgca
    19681 agatcaaggc cccgcacggc accacgctcc gcgccaaatc ctggctcacc gaggcgccgc
    19741 tccgcatgct catgaacaat ctcgacgccg aggtcgccga gcacccggaa tccctcgtcg
    19801 tctatggcgg catcggccgc gccgcccgtg actggcaggc cttcgaccgc atcgtcgcca
    19861 gcctcgaaac cctggaggac gacgaaaccc tcctcgtcca gtccggcaag cccgtcggcg
    19921 tcttcaggac ccaccccgac gcaccccgcg tcctcatcgc caactccaac ctcgtccccc
    19981 actgggccac ctgggaccac ttcaacgccc tcgatcgcca gggcctcatg atgtacggcc
    20041 agatgacggc aggatcctgg atctatatcg gcagccaggg catcgtgcag ggcacctacg
    20101 aaaccttcgt cgaggtcggc cgccgccact atggcggcaa gctcgacggc aaatggatcc
    20161 tgaccggcgg gctcggcggc atgggtggtg cccagccctt ggccgccacc atggccggtg
    20221 cttccatgct cgccgtcgaa tgccgcccgt cgcgcatcga gatgcgcctg aacacgggct
    20281 atctcgacac cgccaccgaa agcctcgacg aggccctctc catcctcgac cgctcctgca
    20341 ggcagaagga gcccatctcc gtcggcctcc tcggcaacgc cgccgagatc ttccccgagc
    20401 tcgtccgccg cggcgtccgc cccgacgtcg tcaccgacca gacctccgcc cacgacccgc
    20461 tccacggcta cctccccgcc ggctggagcc tcgaggaatg ggaggagcgc caggagagcg
    20521 acccccaggg cgtcaccgcc gccgcgaagg aaagcatggt cctgcaggtc caggccatgc
    20581 tcgacttcca ccaccagggc atccccaccc tcgactacgg caacaacatc cgccagatgg
    20641 cgaaggagga aggcctcgcc ggcgccttcg agttccccgg tttcgtcccc gcctatatcc
    20701 gcccgctctt ctgccgcggc atcggcccct tccgctgggc cgccctctcc ggcgaccccg
    20761 aggacatcta caagaccgac gcgaaggtga aagcgctcat ccccaacgac aggcacctcc
    20821 accactggct cgacatggcc agggaacgca tccgcttcca gggcctcccc gcccgcatct
    20881 gctgggtcgg cctcgggctt cgccacaagc tcggcctcgc cttcaacgac atggtcaaaa
    20941 ccggtgagct gaaagccccc atcgtcatcg gccgcgacca tctcgattcc ggctccgtcg
    21001 ccagccccaa ccgcgaaacc gaagccatgc aggacggcac cgacgccgtc tccgactggc
    21061 ccctcctcaa tgcgctgctc aactgcgcct ccggcgccac ctgggtctca atccaccacg
    21121 gcggcggcgt cggcatgggc ttctcccagc acgccggcat ggtcatcgtc gccgacggca
    21181 ccgacgcagc agcccgccgc ctcgaacgaa ccctgtggaa cgaccccgcc accggcgtca
    21241 tgcgccacgc cgacgccggc tacgaggacg ccatcaaatg cgcgaaggaa caggggctga
    21301 atctgccttc gttgtcatga acgaaaaaga gcgggggaac tgagttcccc cgtgcccctt
    21361 ccggactttg gggcggaggt tctcggtatc ggcgtgcgat ggcgcatggc gtggctcgct
    21421 ggcgcaacgc cctgtcatcg attatctttc acgacatgag cgccgacctc gacgacctct
    21481 acgagcgtga cttctacgcc tggacccgcc ggcaggcggc ggcgctgcgt catgccgggg
    21541 tcgagcggat caacacgcag gagcccatcg actgggccaa tctggccgag gaggtggagg
    21601 acttgggcaa cgagcagcgc cgcgccatcg ccagccagct cgaacgcctg atccagcacc
    21661 tcctcaagct ggaattcgcc gaggaccgaa cgccgcgccg aaagtggctc ctctccatcg
    21721 acaatgcccg cgcggaaatc gaacggcgct ggacagcctc catcgcccgt gagatcgagc
    21781 ccaccttgcc cagggcgcac gcccgggcac gccgggccgc cgccctcgag ctcgaggacc
    21841 acggcgagcc caaaacggcg caagccctgc ccgaaggccc gcactacacc ctggagcagc
    21901 tcctcgacga gagctggtac ccgaccgcgg aagaacgata ggaaattcgg actgtttttt
    21961 gggtccacga gactcaatta accattggcc gtttccgcca ccttgggttt gcggccgcgc
    22021 ttggccggcg cgagagtgcg acccaggcgc cgctcgacgt cggcgacgaa gtccgtcgca
    22081 cccagcggcc ggccggtccg cacatgctcg cgcagcacgt cgagccgcca gccaggcggc
    22141 acgtcgtcga gaaagctgcg ccagtcggcg accatatggt cgaggccgcg agactcgacg
    22201 aggccatcct ccgcgatgcc gagatgggca cgagcgctcg accaaggcca gtcctccggc
    22261 cgcgcgacca gccgcgcccg ctccgggttg cgctcgacat accgcagggc cgaaagcgca
    22321 tgcggctcgt ccatggcgca cgagccgaac cggccctgcc acagattgcc acgccacccc
    22381 tggcgccggt tgatggcgaa gctgtggcca cgatgggtgt cggcaatcgc cttcgtcatg
    22441 cccccgctgg tcgtcggcac gaggatcaga tgaacgtggt tggacgtgtg aaccgccccg
    22501 ggtttgccgg aggctggttg gtttgagtct cacgctgcca gaa
//
LOCUS       DAOTFI010000042        22491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2725_length_22491_cov_7.346051, whole genome shotgun sequence.
ACCESSION   DAOTFI010000042 DAOTFI010000000
VERSION     DAOTFI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22491
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2725_length_22491_cov_7.346051"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="P5558_06065"
     CDS             <1..330
                     /locus_tag="P5558_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23906.1"
                     /translation="NAKDFMSLLLAPENMGPMLKGALGRFFPVMPALLDDPFYNDPAD
                     PHRYALHEQFTTVENQPFLQTRNYRYAKVMAEQLWAKATGRILLDGWSTEDAIDELER
                     MHVLLDQ"
     gene            779..1246
                     /locus_tag="P5558_06070"
     CDS             779..1246
                     /locus_tag="P5558_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY23907.1"
                     /translation="MPGGRIGDTYLAARERGVIFDVGHGSGSFGFDTARRMVDQGFWP
                     DVIGSGVHVDCIDGSAHDVLVTMSKLLCLGMPLPEVVRAATATPARVMNHPELGTLAP
                     GSVGDATLLVIDRGEFCYLDSIGAVMAGDQRLRQRGIVLRGGWWDDGRPPDAA"
     gene            complement(1270..2694)
                     /locus_tag="P5558_06075"
     CDS             complement(1270..2694)
                     /locus_tag="P5558_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY23908.1"
                     /translation="MTRLQLATLDNGSTAIDAAAVRALATGLEGGTALPGEPGYDEAR
                     TIWNAMIDRRPGLVVRCRRAEDARRGLRFAAEHGLLLAIRSGGHNIAGNAVCDGGLLV
                     DLSQMRDVTVDAGARRVRVEAGARLADVDAATQAHGLAVPLGINSTTGIAGLTLGGGF
                     GWLSRKHGLTIDSLLAAEIVTPDGRLRRIDARSDPDLFWAIRGGGGNFGVVTAFELKL
                     HPVGPEVLSGLIVHPMSEAPALMREYRRLAADMPDELTVWVVMRKAPPLPFLPEEWHG
                     REVLVFAACYAGDMAAGEKALAPLRALGKPIADVISPHQYVGWQQAFDPLLTDGARNY
                     WKSHNLAGLDDGLIDLLVEAVGRLPSPECEIFVAQLGGAASGVDPAATAYAHRDARFL
                     MNVHTRWREAGDDTRCIAWARELFDRAARFATGGVYVNFMPDDEGGRVKGAFGSSLTR
                     LGRIKAEVDPRNLLRMNMNVRPVV"
     gene            2858..6163
                     /locus_tag="P5558_06080"
     CDS             2858..6163
                     /locus_tag="P5558_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY23909.1"
                     /translation="MAAGEVGAELSIREDQIARLYVDGLSYKEIARDLEISPATVRTH
                     LNTIYRKLEVSSRIELLHRLGRNGNVEIPPAPVPESAPAAGALAERRQLTVMFVDLVD
                     STAIAVGLDAEDMHELLQTYRAAVRREVEAFGGHVAGFPGDGVIACFGWPRALEDAAE
                     RSIRAGLAVIAAVGRLQAPGNTPLAGRIGIATGMVVVDGQRGDADSLAGGTPNLAARL
                     QGIAQPGHVVIAADTCELVGDTFDLEPLGEQLVKGLPKPVPVFRVRGEQLQLNRFEAQ
                     HARKLSPMVGRELELALLVERWERAKEGEGQAVLLIGEPGIGKSRIAAALIERAVADG
                     ARILRYQTSPQQGDSPLWPVRRQLVAEADLERLPDHTARLHRLGELLGDADRELGDAL
                     PLLAELLDIDGTAPPARTSAARRRELVIEILVRRLEQLARRSPVLMLFEDLHWIDPTS
                     LELVERILGRIDEARVLLLLTSRPEDQPRLTGHPHLTAVTLNRLGKAAALKVVERLAG
                     SDRLPDDMVATILARTDGVPLYIEEMTRATLERGGDVASVPASLQDSLMARLERLDAV
                     KEVAQIAACIGREFDHDLLEAAAGEARAGLDEALYRLVGAELVFRRGDRFLFKHALVQ
                     DVAYQSLLRGPRALLHERIAEALLGRFPERAQAEPQTVAHHLEQAGRTEAAIDYYMRA
                     ADLANDKAANREARRYLERSLRLIETLPAGASRDRREVESLTMLGKVQAAIEGHASLS
                     TKEVYSRALERCRAAGLRLAEFPIVLGLTIQAAVSGSADEALALARRLAELASGLDDS
                     TLAVEADYALGITHSWRGELPLARHHLEAARRRYTIDQHPRHLALYGQDPGVVCGCRG
                     ALTLWYMGYADQARHLLDESLALAHRLGHSFSINYALNWNAMLALEAGEPEAAERAVA
                     LTLDHAGEQGFGTWLAFGAVCRARLVLDHGTPASAIEAIEKALAKVTESGSGVFKAYT
                     LSLLGDALRRQGQHDAARDRLAEAVRLLEAGGARWMEAEVLRRRALCERDRGDTAAAL
                     ATLEAALEVARRQEAQMLELRAAMDLTRLRAKLGEPERDATLLAACLAGFTEGFDTRD
                     LREATTLLAELDA"
     gene            complement(6173..7168)
                     /locus_tag="P5558_06085"
     CDS             complement(6173..7168)
                     /locus_tag="P5558_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23910.1"
                     /translation="MRIEGFTEGKNLDDPGANEDQFLILPGRGYAVIDGVTDRTGERY
                     DGMLAGRLAGLVVRDAAAAFLLDPAERACVPERLVAHITAAIQAAYAQHGIGEIARER
                     PARRFGATLALAAHAGEVLRIILVGDSGVRFNGDELVTVDSGLDLVTAGIRQAAWQHL
                     VAAGADHAECARVSRACVFNGLARLHPGMRPWLNEAALADLRQRYLAGAGQRFAHVPQ
                     DDIQLLVDGGIIEGQGLFQNNTKSPLSYCVLDGFPVPMDLVRVIDRPLTGLATLELFT
                     DGYFSAGAEPTVAAWEAAFLEVERTDPDKLDVYPSVKGTVGRVRADDRTVLIVGF"
     gene            complement(7175..8833)
                     /locus_tag="P5558_06090"
     CDS             complement(7175..8833)
                     /locus_tag="P5558_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY23911.1"
                     /translation="MASAGLRLPDLWTVLTLAALAVLAIFVLWPLANVFTASFYGEFS
                     GSLGLENYATFFGSGYFRRALWHSLFTSAMGTLLALALGLPLAVIAARYDFVGKAALR
                     VLVLLSLISPPFIGAYAWIMLLGRQGLVTQALAGIGIGLPTIYGAPGIILVTALNLYP
                     LVFLLVHGALLRIDRSLEEASLGLGRTRFQTFRSITLPLTLPAMATGALLVFLGVLSD
                     FGTPALLGENYPVLATLAFTLFLSEIGGNPAMASTTSVVLVTVALVLILLQRWVASRR
                     NYATDAINRPEILRMAGWQRFAFPAFAWGLMAIANLPLVIIVYTSFLETSGPVFLQSF
                     SLDSYRQVFHLVRGAIVNSFAFSGMALALIVVTGSVVGYIISRRDTMLTRVLDTALMI
                     PYVIPGTVLGIGFITAFNQPPIWITGTVAIIVAAYFIRRLPYMVRACASYVYQIDRSV
                     EEASINLGAPPGKTFMKIMLPLMAPGILAGAVLAWVEVINELSATVVLYTGTTSTMPI
                     ATYSQVLAGSYGPAAAVASLLILATTLSLVLFTVIGGSDRELTV"
     gene            complement(8855..9949)
                     /locus_tag="P5558_06095"
     CDS             complement(8855..9949)
                     /locus_tag="P5558_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23912.1"
                     /translation="MAAIGIVGLVKRFGAVTAIEALDLEVGAGEFFTLLGPSGCGKTT
                     LLRIIAGFERQNAGDLQVGGRLANDIPAHQRDMGMVFQNYAIFPHMSVFDNVAYGLRQ
                     RRFNRGEIGAKVSRALDLVRLGGYERRFPDQLSGGQKQRVVLARALVIEPQVLLMDEP
                     LSALDAKVRVGLRRDIRLVQQELGITTLYVTHDQEEALVVSDRIMVMEAGEIRQLGSP
                     REVYEDPANLFVATFIGTSNLLDGRVDRSGGGSVALALGARLDLPDLQAADGTELHLA
                     LRPEHLTVLDDGPVPEGLVALEGIVELVSYLGSQSRIAVRIPGVAEVVEVEAPSAAPA
                     SALPSGRPVRLGFRPESAFLFAAASGERVR"
     gene            complement(9987..10976)
                     /locus_tag="P5558_06100"
     CDS             complement(9987..10976)
                     /locus_tag="P5558_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23913.1"
                     /translation="MTKSLALGLAVALLAGTALTPGNGAAQGGTVSVYTAHAPEIYNE
                     IVPKFEEATGITVSLVQAGSGDIVQRARAEAASPLADVIWSIGGEALEANSDLLEPYT
                     SSHDAELDPLYKVGTNWLPYTGIPMVLVVNTDMVAADDMPVSWADLTDPKWNQKVAYA
                     GADSSGSAFMQLATILDVYGEEQGWELFGEMMKNFIVTGSSGRVPRGVADGEFAVGLT
                     LEEAAQRYVAGGANVAIVYPEPATSALPDAVALVKGGPNPDAGKQFIDFVLSKEIQEF
                     LVEQMQRRSVRTDVAQPEGLPGLDEIAIQNYDMGWAGAERENIIKRYLGILRQ"
     gene            complement(11082..12293)
                     /locus_tag="P5558_06105"
     CDS             complement(11082..12293)
                     /locus_tag="P5558_06105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY23914.1"
                     /translation="MRIALVAGCPFPWPRGTPARILGMAQALHQAGHEVHVIAYPIGD
                     LDQQLPFPVHRSLGLPFYRRTAPGADPLRLAWFLPALAATVARVVAWQRIEIVHAHHY
                     EGLLAAWPAKRLHGVPVIYDAHTTLAGELPYYRLGVPRRCTTAVGRSLDKRLPKLADH
                     IVATSQYIADELARDGMPTDRVTVVGNGIEDALVAGPAPESRPDGTGERIVYAGNLAG
                     YQGIDTLLEAFTLVAKARPGARLTLLTDSSFDRFAPLAERLGIQDRIDIEPPPPPQAL
                     GRRLAAASLLVNPRAECPGYPLKLLNYMAAGRPIVSFRDSARGLTQDQDAWLVDEPTP
                     AALARGMLAVLDRPDRGASLGRSARERVVAELTWSRVVANLEGLYRRMVAHEPPLADH
                     RSSSVGVTSGD"
     gene            13170..13739
                     /gene="bchJ"
                     /locus_tag="P5558_06110"
     CDS             13170..13739
                     /gene="bchJ"
                     /locus_tag="P5558_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriochlorophyll 4-vinyl reductase"
                     /protein_id="HRY23915.1"
                     /translation="MCPSAVSQVAAVLDERVGEAPVRRLFEAAGLSRYLAGAPTTPVD
                     EREVAALHRTLRRQLGPDCARAVARAAGRRTADRLLARHLPEPLRRLLAHLPRPLVAR
                     LLLLSFERDHWRLAGSGAFVAVYGRPIRLGLTNNPLCPDEHSKAPACDFVAGTVEQLC
                     RSLITPRSSVSEIACKACGAPACIFEIEL"
     gene            13823..14389
                     /gene="idi"
                     /locus_tag="P5558_06115"
     CDS             13823..14389
                     /gene="idi"
                     /locus_tag="P5558_06115"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRY23916.1"
                     /translation="MPGMPDAPAIRPNARIDTELVMLVDVEDRVIGTAEKHAAHRSGA
                     LHRAFSVLIHNPAGNWLLQRRSPGKYHSGGLWTNAACGHPRPGEATEAAAGRRLEEEL
                     GFVCPLAYVTRVHYRSPLDHGMTEHELVSIYTGRHEGPVEPDPVEADAVRWIEPQELR
                     RDMDAHPDRYTVWFRKYVTELWATLHAA"
     gene            complement(14400..14786)
                     /locus_tag="P5558_06120"
     CDS             complement(14400..14786)
                     /locus_tag="P5558_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRY23917.1"
                     /translation="MTSAKFSVALLLAGVVLAISTPVKANDGDPEAGKKVFRQCQACH
                     VADKEQNRVGPHLVGIIGRPAGAVEDFKYSDAMANSGIVWDETTITEYLANPKEYIPG
                     NKMVFAGLRKPEQIQDVIAYLESLDD"
     gene            15032..15667
                     /locus_tag="P5558_06125"
     CDS             15032..15667
                     /locus_tag="P5558_06125"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRY23918.1"
                     /translation="MDLRLADLAARDRYKLLTSLVVPRPIAFVTSQDADGLVNAAPYS
                     FFNVFSDEPPLVVIGIGRRAGGALKDTARNIEAKGEFVINLVDEELAAAMNVAAVDFP
                     PETSEPGPAGLGLAASIVVRPPRIAEAPAALECRNHTTLVIGTDHRLVVGEVLAVHVR
                     DGLVDPGTLRIDFDAYRPVGRLFANLYCSTRDAFELVRENYAQWRRRTGGE"
     gene            15743..17185
                     /gene="hydA"
                     /locus_tag="P5558_06130"
     CDS             15743..17185
                     /gene="hydA"
                     /locus_tag="P5558_06130"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRY23919.1"
                     /translation="MVHCDLVIKGGRVATATDVLEADIGITGETVVAIAKGLPTGRLE
                     IDARGKIVTPGGVDSHCHIEQLAASGLMNADTFETATVSAAMGGTTTVVPFAAQHVGM
                     SLAEVVADYHERAARGAVVDYAFHMILADPRPEILRDELPGLVRDGHSSLKVFLTYDR
                     LRVEDEQFLDVLWAAREHGCMVCVHAENHGMISWMGKRLVAQGLTAPKFHVPSHPRLA
                     EAEAINRAIAMAAFIDQPLMIFHVSTREGAATVRRWQGEGTKVFGETCTQYLTLGATD
                     LDRPGIEGGMWICSPPLREPDDREALWQALGQGTLATVSSDHAPYRMDESGKLARGPN
                     PSFKEMANGMAGIELRLPVLFDAMVSQGRMSLTDFVRLTATEPAKLYNLHPKKGTIAV
                     GSDADLVIWDPEATTTITHDTTHDRSGYSAYVGREIKGWPSHVLRRGAVIVEAGELRA
                     APGSGQFLPRTGGAAATPSGRPAAELAHLG"
     gene            complement(17173..17574)
                     /locus_tag="P5558_06135"
     CDS             complement(17173..17574)
                     /locus_tag="P5558_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23920.1"
                     /translation="MSGRPATSVGLPRRLLLGGLLAIGGAFLAFVAGTAFAARFLVPP
                     GTGLAGPAEAVGYGVLGAAGAVLLAVLIATFAGRRLLLAATLIVVLLALASAAGLAFL
                     AQQSAAPEEELPMPARATTAPGAATGAAQPR"
     gene            complement(17571..19445)
                     /locus_tag="P5558_06140"
     CDS             complement(17571..19445)
                     /locus_tag="P5558_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRY23921.1"
                     /translation="MQLSARSTRWSITVVAVAMSVFHLYVAFVGPPDAYVMRGLHIAF
                     ALALTFLVLPGWNGRAERPGLVDLTLLVIAVAAALYPTWNLGYIVSRMYYVDDPTLWD
                     FVFGIALVLLIMEATRRATGWALPATAAVFLLYGLTWGNQSVGIMLDQLYLTTEGIFG
                     IPLYVSATYVMLFILFGAFVERSGAGQLFMDFALALAGHTSGGPAKVAIITSSLFGTV
                     SGSAVANVMTTGTFTIPLMRRSGYRPAFAGAVEAVASTGGQLMPPIMGAAAFVMAEFL
                     GTSYLTVAAFALLPALLYYVAVFMAVHFEARRVGLVGLPRADLPRLKEVLVERGHLFL
                     PLVVIIGVLLIGYSAAYAAICGIASVVPTTWLRAGTRRTFTPTVIIEALEAGARNTIV
                     VALACACAGIVIGTITLTGLGLSFTNIVLALSQNMLLPALVLTMAAGIVLGMGLPTTP
                     AYIVQVALLVPALVRLGVQVEAAHLFVLYFAVLSAITPPVAMAVYAANAISRAGLMES
                     SWAAIKLGLTGYIIPFMFVFGPSLLMIGEWHQIVLTAITATIGVTCLAAALHQHFFFG
                     PARFWERALLLAAALVLIKPGLWTDALGAALVALTIASQILVRPALVAAPPVERNAVE
                     "
     gene            complement(19525..20469)
                     /locus_tag="P5558_06145"
     CDS             complement(19525..20469)
                     /locus_tag="P5558_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY23922.1"
                     /translation="MKTRHSLAILALGLGLATTAATTAEAQQVKLMTGPQGGSWYPLG
                     GAIANIAGKSGINVQVLPGAGIANVLAVSQGKADIGFANSISTVDGVNGRPPFEAVTE
                     NVCNIGTLYPQYFQIVVNADSGIETVDDAKGQPIAIQPKGNTAEAISAQLFEVHGMTY
                     DDMSQVSFVSYTDAVSLMKDNNAQMFTLGTTVPASAVMDLASARDIRLLEISDEAFAA
                     MRELNPGYSKLTIPAGSYPGQDEDVDVIGYATHVIARCDLDPDVVYKILDGMVTDKAD
                     LVSIVKVMDGTTLEVMASDTGVPMHEGATRFYQEKGAL"
     gene            complement(20552..21397)
                     /gene="fghA"
                     /locus_tag="P5558_06150"
     CDS             complement(20552..21397)
                     /gene="fghA"
                     /locus_tag="P5558_06150"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HRY23923.1"
                     /translation="MALRPTAAHRCHGGVQAVYRHASTTTGTDMEFSVYLPPAAEEGR
                     RCPVLWWLSGLTCTWENATTKAGFQGPAAAAGLIVVCPDTSPRGLDLPGEHDTYDFGS
                     GAGFYLDATVEPWARNYRMESYLTRELQDLVAAELPIDPARQGIFGHSMGGHGALTLA
                     LKNPALYRSVSAFAPIASPMRCPWGEKALAGYLGLDRTKWRAYDATALIEDGAKAAGI
                     LVDQGLGDNFLESQLKPELLEAACATAGIPLELRRHAEYDHSYYFMASFMADHIAWHG
                     RRLGN"
     gene            complement(21397..>22491)
                     /locus_tag="P5558_06155"
     CDS             complement(21397..>22491)
                     /locus_tag="P5558_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HRY23924.1"
                     /translation="VAFAAGKPLEVTTVELEGPRAGEVLVEIMATGLCHTDEFTRSGG
                     DPEGIFPSILGHEGAGIVREVGAGVTSVVPGDHVIPLYTPECRECEYCLNPKTNLCQA
                     IRTTQGQGLMPDGTSRFRLGKDVIHHYMGCSTFSNFTVLPEIAVAKVRKDAPFDKICY
                     IGCGVTTGIGAVINTAKVEPGSNVVVFGLGGIGLNVIQGARLVGADMIVGVDLNPARE
                     ATARKFGMTHFVNPKDLGDKDVVAHLVELTRGGADYSFECIGNVKTMRQALECCHKGW
                     GESVIIGVAGAGQEISTRPFQLVTGRVWRGTAFGGARGRTDVPRIVDWYMERKINIDD
                     LITHTMPLGDINKGFDLMHEGKSIRGVVVY"
BASE COUNT         3648 a         7761 c         7817 g         3265 t
ORIGIN      
        1 aacgccaagg acttcatgag cctcctgctc gcgcccgaga acatggggcc gatgctgaag
       61 ggcgcccttg gccgcttctt cccggtgatg ccggcgctcc tggacgatcc cttctacaac
      121 gacccggcag atccgcatcg ctacgccttg cacgagcagt tcaccacggt cgagaaccaa
      181 ccctttctgc agacgcggaa ctatcgctac gccaaggtca tggcggagca gctctgggcc
      241 aaggccactg gtcgaattct cctcgacggc tggagcaccg aggacgcgat cgacgagctc
      301 gagcggatgc acgtgctgct cgaccagtag tgcaagccgc gctccgagat gacatcacgg
      361 gtgcaagcta cgccgtcgtc gatggtggcg aggccgggcg cctccatgga tcgggcctgg
      421 ggcttgatcc tgttgatccc ctacgccgcc gtcttcgtcc tgttcgtcgt ctatcccatc
      481 gcctacggca tctggctcgg cagcgatatc gccggctacg agcggctgct gaacgatccg
      541 atcgacgggc ggaccgtcag cgacaccgtg gtcttcctga tcgtcggcgt gaatctcaag
      601 atgctggtgg cccgggctct ccagcttcgg tggcgatgtt caccacgccc gacgccgagg
      661 ttccggtcat ggtgcatatc ggcgccgccc gccgccgcgc ctcgaggaca tcgtcgaccc
      721 gctccgcccc ggcgacatcg tcacccactg ctacacgtcc aagatgaact agccctggat
      781 gcccgggggc cggataggcg acacctatct cgcagcccgc gagcgcggcg tgattttcga
      841 cgtcggccac ggctccggct cgttcggctt cgacaccgcg cggcgaatgg tcgaccaggg
      901 tttctggccg gacgtcatcg gcagcggcgt gcatgtcgac tgcatcgacg gctccgccca
      961 cgacgtgctg gtgaccatgt cgaagctcct ctgcctcggc atgccgctgc ccgaggtcgt
     1021 ccgcgccgcc accgccacgc cggcccgagt catgaaccat cccgagctcg gcacgctggc
     1081 gcccggctcg gtcggcgatg ccactctcct cgtcatcgac aggggcgagt tctgctacct
     1141 cgattcgatc ggcgccgtga tggcgggcga ccagcgcctg cgccagcgcg gcatcgtgct
     1201 gcgcggcggc tggtgggacg acgggaggcc gccggatgcg gcctgacgcc ccctcgcccc
     1261 cagacatcgt cagacgacgg gacgaacgtt catgttcatc cgcagcagat tgcgcgggtc
     1321 cacctctgcc ttgatccggc cgagccgcgt caggctcgag ccaaaggcac ccttgacccg
     1381 accgccctcg tcgtccggca tgaaattgac gtagacgcca cccgtagcaa agcgggccgc
     1441 ccggtcgaaa agctcgcgcg cccaggcgat gcagcgcgtg tcgtcgccgg cttcccgcca
     1501 gcgggtgtgc acgttcatga ggaagcgggc atcgcgatgc gcgtaggcgg tggctgccgg
     1561 atcgacgccg cttgctgccc cgccgagctg ggccacgaag atctcgcatt ccggtgacgg
     1621 caaccggccg accgcctcca ccagcaggtc gatcaggcca tcgtccagac cggccaggtt
     1681 gtgggacttc cagtagttgc gcgcaccgtc ggtgagcaac gggtcgaagg cctgctgcca
     1741 gccgacatat tggtgcgggc tgatgacatc ggcaatcggc ttgccgaggg cgcgcaacgg
     1801 cgccagggcc ttctcgcccg ctgccatgtc gccggcgtag caggcggcga agaccagcac
     1861 ctcgcggccg tgccattctt ccggcaggaa cggcagcggc ggcgccttgc gcatcaccac
     1921 ccagacggtg agctcgtccg gcatgtcggc ggccagccgg cgatactccc gcatgagggc
     1981 cggcgcctcg ctcatcggat ggacaatgag gcccgaaagc acctccggcc cgaccgggtg
     2041 cagcttcagc tcgaacgcgg tgaccacacc gaagttcccg ccaccgcccc ggatcgccca
     2101 gaagagatcc ggatcgctcc tggcatcgat tcgccggagg cgtccgtccg gggtgacgat
     2161 ctccgcggct aacaggctgt cgatggtcaa cccgtgcttg cgcgacagcc agccgaagcc
     2221 accgccgagc gtcagcccgg caatgcccgt cgtcgagttg atgccgagcg gcacggccag
     2281 accatgcgcc tgggtcgcgg catcgacatc ggcgagacgg gcacccgcct cgacccgcac
     2341 gcggcgggct cctgcgtcga ccgtcacgtc gcgcatctgc gagagatcga ccagcagccc
     2401 gccatcgcag acggcattgc cggcgatgtt gtggccgcca ctgcggatcg cgagcaggag
     2461 gccgtgctcg gcggcgaagc ggagtccccg tcgggcgtcc tccgccctcc ggcagcggac
     2521 cacgagaccg ggccttcggt cgatcatggc gttccagatg gtccgcgcct cgtcgtagcc
     2581 cggctcgccg ggcagcgccg tgccgccctc gaggcccgta gcgagcgccc tcaccgccgc
     2641 cgcatcgatg gcggtcgagc cgttgtccag ggtcgcgagc tgcagtcggg tcatggtatt
     2701 ctcccttgcc atgcgtgtga ggcccaagct ggcatcgccg gccggcgcgt ggtatcggtc
     2761 aggtagcgta gaaagcggcg cctcgatgac gtaggtcgcc tgtgcgccga atagtcgggt
     2821 atggccgtca ggtctcgaca atccggggga atcgcgattg gcagccggtg aagtcggcgc
     2881 cgaattgagc attcgcgagg accagatcgc gcggctttat gtcgacggtc tgagctacaa
     2941 ggagatcgcg cgcgatctcg agatctcgcc ggcgaccgtc cgcacccacc tcaataccat
     3001 ctaccgcaag ctggaagtgt cgagccggat cgagctactg caccgcctcg gtcgcaacgg
     3061 caacgtcgag atcccgccgg cgcctgttcc ggaatccgcc cccgcggccg gagccctagc
     3121 cgaacggcga caactcactg tgatgttcgt ggatctggtc gactcgaccg cgatcgccgt
     3181 cggactcgac gccgaggaca tgcacgagct gctgcagact tatcgggccg ccgtacggcg
     3241 ggaggtcgag gccttcggcg ggcatgtcgc cggctttccg ggtgatgggg tgatcgcctg
     3301 cttcggctgg ccgcgcgcac tcgaggacgc agccgaacgg tcgattcgtg ccggcctcgc
     3361 cgtcatcgcc gccgtcggcc gcctccaggc acccggcaac accccgctcg ccggccggat
     3421 cggcatcgcc accggcatgg tcgtcgtcga cggacagcgc ggcgacgccg acagcctcgc
     3481 cggcggcaca cccaatctcg cggcccggct tcagggcatc gcccagcccg gccatgtcgt
     3541 cattgccgcc gacacgtgcg agttggtcgg cgataccttc gatctcgagc ccctgggcga
     3601 gcagctcgtc aaggggctgc cgaagccggt gccggtcttc agggtcaggg gcgaacagct
     3661 gcagctcaac cgcttcgagg cgcagcatgc ccgcaagctg tcgccgatgg tcggccgcga
     3721 actggagctg gcgctgctcg tcgagcggtg ggagcgcgcc aaggaaggcg aaggccaggc
     3781 cgtgctgctg atcggcgagc cgggcatcgg caagtccaga atcgccgcgg cattgatcga
     3841 gcgagcagtg gcggatggcg cgcggattct gcgctaccag acgtcgccgc agcagggcga
     3901 tagcccactc tggccggtgc gtcgccagct cgtcgccgag gccgacctcg aacggctgcc
     3961 cgaccacacg gcacggctgc accggctcgg cgagcttctc ggtgacgccg atcgcgagct
     4021 cggcgatgcg ctgccgctgc tggccgagct gctcgacatc gacgggaccg cgcctcctgc
     4081 ccggacgagc gcagccaggc ggcgcgagct cgtgatcgag atcctggtga ggcgcctcga
     4141 gcagctcgcc cggcgctcgc cggtgctgat gctgttcgag gatctgcact ggatcgaccc
     4201 gacctcgctc gagctggtcg agcgcatcct cggccggatc gacgaggcaa gggtgctgct
     4261 cctcttgacc tcgcggccgg aggaccagcc gcgcctcacg ggccatccgc acctcacggc
     4321 cgtaaccctg aaccggctcg gcaaggctgc cgccctgaag gtcgtcgagc ggctggccgg
     4381 cagcgaccgg ctgcccgacg acatggtcgc gacgatcctc gcccgcaccg acggcgtgcc
     4441 cctctatatc gaggagatga ccagggccac cctcgagcgg ggcggcgatg tcgccagcgt
     4501 tccggcctcg ctgcaggatt cgctgatggc ccgcctcgag cgcctcgacg ccgtcaagga
     4561 ggtggcgcaa atagccgcct gcatcggccg cgagttcgat cacgaccttc tcgaggccgc
     4621 cgctggcgag gccagggccg gcctcgacga ggcgctctac cgcctggtcg gcgccgagct
     4681 cgtctttcgc cgcggcgacc gcttcctctt caagcacgcg ctggtccagg acgtcgccta
     4741 ccagtcgctc ctgcgcggcc ctcgcgcgct cctgcacgag cggatcgccg aagccctgct
     4801 cggccgcttc ccggagcgtg cccaggccga gccgcagacg gtggcgcacc atctggagca
     4861 ggccgggcgc acggaggcgg cgatcgacta ctacatgcgg gccgccgatc tcgccaacga
     4921 caaggccgcc aatcgcgagg cccgccgcta tctcgagcgc tcgctgcggc tgatcgagac
     4981 gctgccggcg ggcgcctcgc gcgaccggcg cgaggtcgag tccctgacca tgctgggcaa
     5041 ggtgcaggcc gccatcgaag gccatgcgag cctctcgacg aaggaggtct acagccgggc
     5101 gctcgagcgc tgccgcgcgg ccgggctccg cctcgccgag ttcccgatcg tcctcgggct
     5161 gaccatccag gcggccgtgt cgggcagcgc ggacgaggcg ctcgccctgg cccgccggct
     5221 ggccgagctg gcgagcgggc tggacgactc gacgctcgcc gtcgaggccg actacgcgct
     5281 cggcatcacc cattcctggc gcggcgagct gccgctcgcc cgccatcatc tcgaggccgc
     5341 ccgccggcgc tacacgatcg accagcaccc gcgccatctg gcgctctacg gccaggaccc
     5401 gggcgtggtc tgcggctgcc gcggcgcctt gaccctctgg tacatgggct atgccgacca
     5461 ggcccggcac ctgctcgacg aaagcctcgc cctcgcccat cggctcggcc attccttcag
     5521 catcaactac gccctcaact ggaacgccat gctggcgctg gaggcgggcg agcccgaggc
     5581 ggccgaacgg gcggtggccc tgacgctcga ccacgccggc gagcagggct tcggcacctg
     5641 gctggcgttc ggcgcggtct gtcgcgcccg tctcgtgctc gatcacggca ctccggcatc
     5701 ggccatcgag gcgatcgaga aggcgcttgc caaggtcacc gagtccggca gcggcgtttt
     5761 caaggcctac acgttgagcc tcctcggcga cgcgctgcgc cgccagggcc agcacgacgc
     5821 ggcgcgggac cggctggccg aggccgtgcg tctcctcgag gcgggcgggg cccgctggat
     5881 ggaggccgag gtgctgcgcc ggcgcgcgct ctgcgagcgc gaccggggcg acacggcggc
     5941 ggccctggcg accctcgagg cagcgctcga agtcgcccgc cgacaggagg cgcagatgct
     6001 cgagctgcgc gccgcgatgg acctcacccg tctccgggcg aagctgggcg agcccgaaag
     6061 ggacgccacc ttgctcgctg cctgcctcgc cggcttcacc gagggattcg acacccggga
     6121 cctgcgcgag gccacgacgc tgctcgccga gctggatgct tgaacggccg cctcaaaagc
     6181 cgacgatgag caccgtcctg tcgtcggcgc gaacgcggcc gacagtgccc ttgaccgaag
     6241 gatagacgtc gagcttgtcc gggtcggtgc gctcgacctc caggaacgcc gcctcccagg
     6301 cggcaaccgt gggctcggcg ccggcgctga aatagccatc cgtgaagagc tcgagcgtgg
     6361 cgaggccggt cagcggccga tcgatcaccc gcacgaggtc catcggcacg ggaaagccgt
     6421 cgagcacgca gtagctcaag gggctcttcg tgttgttctg gaagagcccc tgcccctcga
     6481 tgatgccgcc atcgaccaag agctggatgt cgtcttgcgg gacatgggcg aagcgctgcc
     6541 cggccccggc gaggtagcgt tggcgcaggt ccgcgagcgc ggcctcgttg agccagggcc
     6601 gcatccccgg gtgcagtcgg gcgagaccgt tgaagacgca ggcgcgcgag acgcgggcgc
     6661 attcggcgtg gtcggcgccc gcggccacga ggtgctgcca ggcggcttgg cggatgccgg
     6721 ccgtcaccag gtcgagaccg gaatcgaccg tgaccagctc gtcgccgttg aagcgcacgc
     6781 cgctgtcccc gacgaggatg atgcgcagga cttcgccagc atgtgccgcc aatgccaggg
     6841 tggcgccgaa gcgccgcgcc ggccgctctc tggcgatctc gccgatgccg tgctgggcat
     6901 aggccgcctg gatcgccgcg gtgatgtgcg ccaccagccg ctcgggcaca catgcccgct
     6961 cggccgggtc gagcaggaag gcggcggccg catcacgcac gacgagcccg gcgaggcggc
     7021 ccgccagcat gccgtcatag cgctcgccgg tgcggtcggt gaccccgtcg atcaccgcgt
     7081 agccacgtcc cggcaggatc aggaactggt cctcgttggc gcccggatcg tcgagattct
     7141 tgccctcggt gaaaccttcg atgcgcatga ccggtcagac cgtgagctcg cggtcgctgc
     7201 caccgatcac ggtgaagagc accagcgaga gagtggtcgc gagaatcagc aggctggcga
     7261 cggcggcggc cggaccatag gagccggcca ggacctggct gtaggtcgcg atcggcatgg
     7321 tcgaggtggt gccggtgtag agcacgaccg tggccgagag ttcgttgatg acctcgaccc
     7381 aggcgagcac ggccccggcg aggatgcccg gcgccatgag gggcagcatg atcttcatga
     7441 aggtcttgcc cggcggggcg cccagattga tcgacgcttc ctcgaccgac cggtcgatct
     7501 ggtagacgta ggacgcgcag gcgcggacca tgtagggcag ccggcggatg aagtaggcgg
     7561 cgacgatgat ggccaccgtg ccggtgatcc agatcggtgg ctggttgaag gcggtgatga
     7621 agccgatgcc gagcacggtg cccggaatga cgtaggggat catcagggct gtatcgagca
     7681 cgcgggtgag catcgtgtcg cgacggctga tgatgtagcc gaccacgctg ccggtcacca
     7741 cgatcagcgc cagcgccatg ccggagaagg cgaagctgtt gacgatcgcc ccgcgcacca
     7801 gatggaagac ctgccggtag ctgtcgagcg agaaggactg gaggaaaacc gggcccgagg
     7861 tctcgaggaa cgaggtgtag acgatgatca cgagcggcag gttggcgatc gccatcaggc
     7921 cccaggcgaa ggcgggaaag gcgaaccgct gccagcccgc catgcgcagg atctcgggcc
     7981 ggttgatggc gtcggtggca taattgcgcc gcgacgcgac ccagcgctgc agcaggatga
     8041 gcacgagggc aaccgtgacc agcaccacgc tcgtggtcga cgccatggcc gggttgccgc
     8101 cgatctcgga gagaaagagc gtgaaggcca gggtcgccag caccggatag ttctcgccga
     8161 gcagcgccgg cgtgccgaaa tccgagagca cgccgagaaa gaccagcaag gcacccgtcg
     8221 ccatggccgg caaggtgagc ggcagggtga tgctgcggaa ggtctggaaa cgcgtgcgac
     8281 cgaggccgag cgacgcctcc tcgagcgagc ggtcgatccg cagcagggca ccgtgaacca
     8341 ggaggaagac gagcgggtag aggttgagcg ccgtgacgag gatgatcccg ggggcgccat
     8401 agatggtcgg caggccgatg ccgatcccgg ccagggcctg ggtcaccagc ccctgccggc
     8461 cgagcagcat gatccaggca taggcgccga tgaagggtgg cgagatgagc gaaaggagga
     8521 cgagcacgcg caacgccgcc ttgcccacga aatcatagcg ggcggcgatg accgcgagcg
     8581 gcaggcccag tgccagagcc agcagcgtgc ccatggccga ggtgaagagg ctgtgccaga
     8641 gagcacgacg gaaatagccg gagccgaaga aggtggcgta gttctcgagc ccgaggctgc
     8701 cgctgaactc gccgtagaag ctggcggtga agacattggc gagcggccag agcacgaaga
     8761 tcgcgagcac ggcgagtgct gccagtgtca gcaccgtcca gagatcgggg agacgcagcc
     8821 cggccgacgc caccttcctc cgggcggcca tagctcagcg gacccgctcg ccactggccg
     8881 cagcgaagag gaacgccgac tcggggcgaa accccaggcg caccggccgt cccgacggca
     8941 gggcgctggc gggtgcagcg ctcggcgcct cgacctccac cacctcggcg acgccgggga
     9001 tccggacggc gatccgcgac tgcgagccca ggtagctgac gagctcgact atgccctcca
     9061 gagccaccag cccttccggc accggcccgt catcgagaac cgtgaggtgc tcgggacgca
     9121 gcgccaggtg aagctcggtg ccgtcggcag cctgcagatc cggcaggtcg agccgtgcgc
     9181 cgagggccag cgcgacactt ccgccacccg accgatcgac ccggccgtcg agcaggttgc
     9241 tcgtgccgat gaaggtggcg acgaagagat tggccgggtc ctcgtagacc tcgcgcgggc
     9301 tgccgagctg gcggatctca ccagcctcca tcaccatgat ccggtcggag acgacgagcg
     9361 cctcctcctg gtcgtgggtc acatagagcg tggtgatgcc gagctcctgc tggaccaggc
     9421 ggatgtcccg ccggagcccg accctgacct tggcgtcgag cgccgaaagg ggctcgtcca
     9481 tgagcaggac ctggggctcg atgacgaggg cgcgggccag gaccacgcgc tgcttctggc
     9541 cgcccgagag ctggtccgga aagcggcgct cgtagccccc gagccgaacc agatcgagag
     9601 cccggctcac cttcgccccg atctcgccgc gattgaaacg gcgctgcctc aggccgtagg
     9661 cgacgttgtc gaagaccgac atgtgcggaa agatggcgta gttctggaag accatcccca
     9721 tgtcgcgctg gtgcgccggg atgtcgttcg cgagccggcc gccgacctgg agatcgcccg
     9781 cgttctgccg ctcgaagccg gcgatgatgc gcaggagcgt ggtcttgccg cagccggacg
     9841 ggccgaggag cgtgaagaac tccccggcgc cgacctccag atcgagcgcc tcgatggcgg
     9901 tgaccgcacc aaagcgcttg accagcccga caatgccgat cgccgccatc agggcgaggc
     9961 gggcaaccgg ctcgaatccg ccaggctcac tggcgcagga tgccgagata ccgcttgatg
    10021 atgttctcgc gctcggcacc tgcccaaccc atgtcgtagt tctggatggc gatctcatcc
    10081 aggccgggca ggccttccgg ctgcgccacg tcggtccgga ccgagcggcg ctgcatctgc
    10141 tccaccagga actcctggat ctccttcgac agcacgaagt cgatgaactg cttgccggca
    10201 tccgggttgg ggccaccctt gaccaaggcc accgcgtccg gcagcgccga ggtcgcaggc
    10261 tccgggtaga cgatggcgac attggcgccg cccgcgacat agcgctgagc cgcctcctcg
    10321 agcgtcaggc cgaccgcgaa ctcgccgtca gcgacaccgc gcggcacccg ccccgagctg
    10381 ccggtgacga tgaagttctt catcatctcg ccgaacagct cccagccctg ctcctcgcca
    10441 tagacgtcga gaatcgtggc gagctgcatg aaggccgagc ccgagctgtc ggcaccggca
    10501 taggcgacct tctggttcca cttgggatcg gtcagatcgg cccagctcac cggcatgtcg
    10561 tccgcggcga ccatgtcggt gttgaccacg agcaccatgg ggatgccggt gtagggcagc
    10621 cagttggtgc cgaccttgta gagcgggtcg agctcggcgt cgtgcgagct cgtatagggc
    10681 tcgagcagat cgctgttcgc ctcgagagcc tcgccgccga tcgaccagat gacgtcggcg
    10741 agcgggctcg ccgcctcggc ccgtgcccgc tgcacgatgt cgcccgagcc ggcctggacc
    10801 agcgacacgg tgatgccggt cgcctcctcg aacttcggca cgatctcgtt gtagatctcg
    10861 ggcgcgtgcg ccgtgtagac cgagacggtg ccgccctgcg ccgcaccgtt ccccggtgtc
    10921 agcgccgtcc ccgccagcag cgcaacagca agaccgagtg ccaggctctt cgtcattcgc
    10981 tctctccccg tgtggcgcgc cagcccgcca ctctccgttt gtggccgttc aatgactgac
    11041 ttctagggca tccgccgccg ggacgatagc atcgacgctc cttagtcgcc gctggtgacg
    11101 cctaccgatg atgacctgtg atcggccagc ggcggctcgt gcgcgaccat gcggcggtag
    11161 agcccctcga gattggccac cacccgcgac caggtcagct ccgccaccac ccgctccctg
    11221 gcagaccgac cgagcgacgc tccgcgatcc ggccggtcca gcaccgcgag catgccccgc
    11281 gccagcgccg ccggcgtggg ctcgtcgacg agccaggcat cctgatcctg ggtcagcccc
    11341 ctggcggagt cccgaaacga gacgatcggc cggcccgccg ccatgtagtt gagcagcttc
    11401 agcgggtagc ccgggcactc ggcccgggga ttgacgagca gcgaagcggc ggcgagccga
    11461 cggccgagcg cctgcggcgg cggcggcggc tcgatatcga tccggtcctg gatgcccagc
    11521 cgctctgcca gcggagcgaa gcggtcgaag gacgaatcgg tcaagagggt gagcctggcg
    11581 ccgggtcggg ccttcgccac cagggtgaac gcctcgagca gcgtgtcgat cccctgatag
    11641 ccggcgagat tgccggcgta gacaatccgc tcgccggttc catcgggccg gctctccggc
    11701 gccggaccgg cgacgagagc atcctcgata ccgttgccca ccaccgtgac ccggtcggtc
    11761 ggcatgccgt cccgggcgag ctcgtcggcg atgtactggc tcgtcgccac gatgtgatcg
    11821 gcgagcttcg gcaggcgctt atcgagggac cgaccgactg ccgtcgtgca gcggcgcggc
    11881 acgccgagac ggtaataggg cagctcgcca gccagggtcg tgtgcgcatc gtagatcacc
    11941 ggcacgccgt gcagccgctt ggcgggccag gcggcgagca atccctcgta gtggtgggca
    12001 tgcacgatct cgatccgctg ccaggcgacc acccgcgcca cggtggccgc cagcgccggc
    12061 agaaaccagg cgagccgcag gggatcggca ccaggtgcgg tgcgtcgata gaacggcagg
    12121 cccagactgc gatgcaccgg gaacggcaat tgctggtcca gatcgccgat cgggtaggcg
    12181 atcacatgga cctcgtgccc ggcctgatgc agcgcctgcg ccatgccgag aatgcgcgcc
    12241 ggggtgcccc gcggccatgg aaacgggcaa cccgcgacca gtgcgatgcg caagggcgac
    12301 gcgcctccca tcagcggcaa tcgaccagga cgggcggcga cccggcccgg cgcaatccgc
    12361 actccaccgc cggcccgctg cccggagcat tgaaatatgc ccgacccgcc atcatcgtgg
    12421 gtccgtcagc ggccgccgag ccggcgcgcc ctgccgattt ttcgattgaa ccatgcgagg
    12481 aagctctgtt gcgcggatct gaccgcattg actggacccc gtaccaactc actctggtcc
    12541 gggcaagcca agcggtcttc cccccatcca gcaccgaacc gaggcatcca atcacgctgc
    12601 gcccataaag aacacgcctc gcaagaaagg cgatcacatc ccgaaccaaa gcattcccga
    12661 ctgccctatc atgctcgacc acacggttac tggcacaacg tcccgtcgat ggaaaggccg
    12721 gcgcgccgcc agatccggcg ccgtcgccag ccaatgggga gggccaagct cgccgttgcc
    12781 ccggcagcgc gccgccttca agacccagcg gtttctcgca ggcgaaagca ccgatcctgg
    12841 cccttgttgc gacggacccg atccacgcgg aaagatcggg ccggccgatt gacctcgatc
    12901 aaagtagagc tagctttcgg cccctagacc aatcgtcaac cagttccatc gggcgacctt
    12961 ccctcgggtc cgccgtcgtc gggtgcttcc ggcgcagcag gattgccgtg cggcccgctg
    13021 caaccgaaag ccgagccgcg tgcagacgtg acaatgaccg agaacgctcg aacggccgag
    13081 actgcggctt gaggagttcc atgcacacgc cggacctcgc caccgttgcc tcaccgccga
    13141 tcggggccgt caccggacag gttggtcgca tgtgcccaag cgcggtttcc caggtggcgg
    13201 ccgtgctgga cgaacgggtc ggcgaggcgc cggtgcgtcg cctgttcgag gcagccggcc
    13261 tcagccgcta cctcgccggg gcccccacga ctccggtcga cgagcgtgag gtggcggccc
    13321 tgcatcggac gctgcgccgc cagctcggcc cggactgtgc cagggccgtg gctcgggcag
    13381 ccggacgtcg tacggccgac cgcctgctgg cacggcacct gcccgagccc cttcgccgcc
    13441 tgctcgcgca tctgccacgc ccgctcgtcg cccggctgct gcttctcagc ttcgagcgcg
    13501 atcactggcg gctcgccggc agcggcgcct tcgtcgccgt ctacgggcga ccgatccgcc
    13561 tcggcctcac caacaaccca ctctgccccg acgagcacag caaggcacct gcctgcgact
    13621 tcgtggccgg caccgtcgag cagctctgcc gcagcctgat cactccccga agctccgtca
    13681 gcgagatcgc ctgcaaggcc tgcggggcac ccgcctgcat cttcgagatc gagctctagc
    13741 gatcaggcgg cgccaagcgc cgccgacccg gcttgacttc gcgccaaggc gagtgtcgac
    13801 tccgaggtac aacggaggaa acatgccggg aatgcccgac gcgccggcga tccggcccaa
    13861 tgctcggatc gacaccgagc tggtcatgct cgtcgatgtc gaggaccggg tgatcggcac
    13921 cgcggaaaag cacgccgcgc accgctcggg ggccctgcac cgggccttct cggtcctgat
    13981 ccataacccc gccggcaact ggctgctgca gcggcgcagc cccggcaagt accattcggg
    14041 cgggctatgg accaacgccg cctgcggcca cccgcgcccc ggcgaggcga ccgaggccgc
    14101 cgcgggccgt cggctcgagg aggagctcgg cttcgtctgc ccgctcgcct acgtcaccag
    14161 ggtccattac cgctcgccgc tcgatcacgg catgaccgag cacgaattgg tcagcatcta
    14221 taccggccgg catgaagggc cggtagagcc cgaccccgtc gaggccgacg cggtgcgctg
    14281 gatcgagcct caggagctgc gccgcgatat ggacgcgcat cccgaccgct acacggtctg
    14341 gttccgcaag tacgtgacgg agctctgggc gacgttgcac gccgcctgag cggctgtcgc
    14401 tagtcgtcca gactctcgag ataggcgatc acgtcctgga tctgctcggg cttgcgcagg
    14461 cccgcgaaca ccatcttgtt gcccgggata tattccttcg ggttggcgag gtactccgtg
    14521 atcgtcgtct cgtcccagac gatgccgctg ttcgccatgg cgtccgagta cttgaagtcc
    14581 tccaccgcgc cggccggccg gccgatgatg ccgacgaggt gcggccctac ccggttctgc
    14641 tccttgtcgg ccacatggca cgcctggcac tgcctgaaca ccttcttgcc ggcctcggga
    14701 tcgccgtcgt tcgccttcac cggcgtggag atcgccagaa ccacgcctgc cagcaacagg
    14761 gccacactga atttcgcgct cgtcaacaga tcatcctccc aaagacagcg acaccggaca
    14821 cctgccgata tacacctggc acagaagtcg gcaagcagga tacgcgcgag accaaaggtc
    14881 gccggcaccg gcccttgccg gcgaccctcg ggagggtcgc cctcggctgc cacccgggct
    14941 agcatgccgc catcgccccg tcggagccac ccatgcccaa tcggcaaaac cgttttatcc
    15001 gcatcgccga caccgggtcg ggcgcagcct gatggacctc cgcctggcgg atctcgccgc
    15061 ccgcgaccgc tacaagctgc tcacctcgct cgtggtgccg cggccgatcg ccttcgtcac
    15121 gtcgcaggat gcggatggcc tggtcaatgc agcgccctat tcgttcttca acgtcttcag
    15181 cgacgagccg ccgctcgtcg tcatcggcat cggccggcgc gccggtgggg cgctgaagga
    15241 cactgcgcgc aatatcgagg ccaaaggcga gttcgtgatc aacctggtcg acgaagagct
    15301 cgcggccgcc atgaacgtcg ccgccgtcga cttcccgccg gagacgagcg agcccgggcc
    15361 tgcgggcctc ggcctagccg cctcgatcgt cgtccgcccg ccgcgcatcg ccgaggcccc
    15421 cgcagcgctg gaatgcagga accacacgac gctggtcatc ggcaccgacc accgcctcgt
    15481 cgtcggcgag gtcctcgccg tccacgtccg ggacggtctc gtcgatcctg gcaccctgcg
    15541 catcgacttc gacgcctacc gcccggtcgg ccggctcttc gccaacctct actgcagcac
    15601 ccgcgacgcc ttcgagctgg tgcgcgagaa ctacgcccag tggcgtcgcc gcacgggcgg
    15661 ggagtaggcg acagacccgc catggccgcc cgcgcccgcc catgctaccg aagggtcgga
    15721 gccaacgggg gagacggtcg gcatggtgca ttgcgatctg gtcatcaagg gcggcagggt
    15781 cgccaccgcc acggacgtgc tcgaggccga tatcggcatc accggcgaga ccgtggtggc
    15841 catcgcgaag gggctgccga ccgggcggct ggagatcgac gcgcgcggca agatcgtgac
    15901 gccgggcggc gtcgacagcc actgccacat cgagcagctc gccgcctccg ggctgatgaa
    15961 cgccgacacc ttcgagaccg cgaccgtctc ggccgccatg ggcggcacga ccacagtcgt
    16021 ccccttcgcc gcccagcatg tcggcatgag cctcgccgag gtcgtcgccg attaccacga
    16081 gcgcgcggcc agaggtgcgg tggtcgacta cgccttccac atgatcctcg ccgatccgcg
    16141 gccggagatc ctgcgggacg agctgccggg cctcgtgcgc gacggccact cgtcgctcaa
    16201 ggtcttcctc acctacgacc gcctcagggt cgaggacgag cagttcctcg acgtgctctg
    16261 ggcggcgcgc gagcatggct gcatggtctg cgtccatgcc gagaaccacg ggatgatctc
    16321 ctggatgggc aagcggctgg tggcccaggg cctgacggcg ccgaagttcc acgtcccgtc
    16381 gcacccgcgc ctcgccgagg ccgaggcgat caaccgggcg atcgccatgg cggccttcat
    16441 cgaccagccc ctgatgatct tccacgtcag cacgcgcgag ggtgcggcga ccgtccgccg
    16501 ctggcagggt gaagggacca aggtcttcgg cgagacctgc acgcagtacc taacgctcgg
    16561 cgccaccgat ctggatcggc ccgggatcga ggggggcatg tggatctgca gcccgccctt
    16621 gcgtgagccc gacgaccgcg aagccctctg gcaggcgctc ggccagggca cgctcgccac
    16681 cgtctcctcg gaccacgccc cctatcgcat ggacgagagc ggcaagctcg ccaggggccc
    16741 gaacccgagc ttcaaggaga tggccaatgg catggccggc atcgagcttc gcctgccggt
    16801 gctgttcgac gccatggtca gccagggccg catgagcctc accgacttcg tccgcctgac
    16861 ggcaaccgag ccggcgaagc tctacaacct gcatccgaag aagggcacga tcgccgtcgg
    16921 cagcgacgcc gacctcgtga tctgggaccc cgaggcgacc accacgatca cccacgacac
    16981 cacccatgac cgctccggct acagcgccta tgtcgggcgc gagatcaagg gctggcccag
    17041 ccatgtgctg cgccgcgggg cggtgatcgt cgaggctggc gagctgcggg cagcccccgg
    17101 ctcggggcag ttcctgccgc gcaccggcgg cgctgcggcg acaccttccg gccggccggc
    17161 cgcggagctc gctcacctcg gctgagcggc gccggtggct gccccgggtg cggtggtcgc
    17221 ccgcgctggc atgggcaatt cctcctccgg tgctgccgac tgctgtgcaa ggaaggcgag
    17281 gccagcggcc gaggccagcg ccaggagcac gacgatcagg gtggcggcga gcagcaggcg
    17341 acgcccggcg aaggtggcaa tgagcacggc gagcagcacg gcgccggcgg caccgagcac
    17401 gccgtagccc accgcctcgg ccggcccggc gagaccggtg cccggcggca cgaggaagcg
    17461 ggcggcaaat gccgtgccgg cgacgaaagc gagaaaggcg ccgccaatgg ccaggaggcc
    17521 gccgagcagg aggcgccgcg gcaacccgac cgacgtggcg ggccgtccgc tcattcgacc
    17581 gcgttgcgtt cgaccggtgg cgccgcgacc agggccggcc tgaccaggat ctggctggcg
    17641 atggtgagcg ccacgagggc ggcacccagc gcgtccgtcc agaggccggg cttgatcagc
    17701 acgagggccg cggcgagcag gagggcgcgc tcccagaagc gcgccggccc gaagaagaag
    17761 tgctggtgca gggcggcggc gaggcaggtg acgccgatgg tggcggtgat cgcggtcagc
    17821 acgatctggt gccactcgcc gatcatgagg agcgacgggc cgaagacgaa catgaagggg
    17881 atgatgtagc cggtgaggcc gagcttgatc gcggcccagg agctttccat gagcccggcg
    17941 cggctgatgg cgttggcggc atagacggcc atggcgaccg gcggcgtgat ggccgacagc
    18001 acggcgaaat agaggacgaa gagatgcgcc gcctcgacct ggacgccgag ccggaccagc
    18061 gccggcacga gcagcgccac ctggacgatg taggcgggcg tcgtcggcag ccccatgccg
    18121 agcacgatgc cggcggccat ggtgaggacc agggccggca gcagcatatt ctgggacagc
    18181 gccaggacga tgttggtgaa cgagaggccg aggccggtca gcgtgatggt gccgatgacg
    18241 atgccggcgc aggcgcaggc cagcgccacg acgatggtgt tgcgtgcccc ggcctcgagc
    18301 gcctcgatga tcaccgtggg cgtgaaggtg cggcgcgtgc cggccctgag ccaggtggtc
    18361 ggcacgaccg aggcgatgcc gcagatggcg gcataggcgg cgctgtagcc gatgagcagc
    18421 acgccgatga tgacgacgag gggcaggaag agatggccgc gctcgaccag cacctccttg
    18481 agccgcggca ggtcggcacg cggcaggccg acgaggccga cgcggcgggc ctcgaagtgc
    18541 accgccatga agacggcgac gtagtagagc agcgccggca gcagggcgaa ggcggccacc
    18601 gtgaggtagc tcgtgccgag gaactcggcc atgacgaagg cggccgcccc catgatcggc
    18661 ggcatgagct ggccgccggt ggaggccacg gcctcgacgg cgccggcgaa ggccggccga
    18721 tagccggagc ggcgcatcag cgggatggtg aaggtgcccg tggtcatgac gttggcgacg
    18781 gcgctgccgg agaccgtgcc gaagagcgag ctcgtgatga tcgccacctt ggccgggccg
    18841 ccggaggtgt ggccggcaag cgcgagggcg aagtccatga agagctgccc cgcgcccgag
    18901 cgctcgacga aggcgccgaa caggatgaag agcatcacgt aggtggcgga cacgtagagc
    18961 gggatgccga agatgccttc ggtcgtcagg tagagctggt cgagcatgat gccgaccgac
    19021 tggttgcccc aggtgagccc gtagagcagg aagacggccg ccgttgccgg cagcgcccag
    19081 ccggtggcgc gcctggtcgc ctccatgatg agcaggacga gggcgatgcc gaagacgaag
    19141 tcccagaggg tcgggtcatc gacatagtac atccggctga cgatgtagcc gaggttccag
    19201 gtgggataga gggccgccgc cacggcgatg acgagcagcg tcaggtcgac gaggcccggt
    19261 cgctcagccc ggccgttcca gccgggcagg acgaggaagg tgagcgccag ggcgaaggcg
    19321 atgtgcaggc cgcgcatgac ataggcgtcg ggcggcccga cgaaggcgac gtagaggtgg
    19381 aagaccgaca tcgccacggc gacgacggtg atcgaccagc gggtgctgcg tgccgagagc
    19441 tgcatacggc ctcttctccc gctcgttgac cgatgccggc gcccgcctca caggggcgcg
    19501 gcgccggcgt ccaggcctag ggcgtcagag cgcacccttc tcctggtaga agcgggtggc
    19561 accctcgtgc atcggcacgc cggtgtcgga ggccatcacc tcgagcgtgg tgccgtccat
    19621 gaccttcacg atcgagacca ggtccgcctt gtcggtcacc atgccgtcga ggatcttgta
    19681 gacgacgtcc gggtcgagat cgcagcgcgc gatgacgtgc gtggcatagc cgatgacgtc
    19741 gacgtcctcg tcctggcccg ggtagctgcc ggccgggatg gtcagcttcg agtagccggg
    19801 gttgagctcg cgcatggcgg cgaaggcttc gtcggagatc tcgaggagcc tgatgtcgcg
    19861 ggccgaggcc aggtccatga ccgccgaggc gggcacggtc gtgccgaggg tgaacatctg
    19921 ggcgttgttg tccttcatca gcgacacggc gtccgtgtac gacacgaagc tcacctggct
    19981 catgtcgtca taggtcatgc cgtgaacctc gaagagctgc gcgctgatcg cctcggccgt
    20041 gttgcccttc ggctggatgg cgatcggctg gcccttggcg tcgtcgaccg tctcgatgcc
    20101 ggaatcggca ttgacgacga tctggaagta ctgcgggtag agggtgccga tattgcagac
    20161 gttctcggtc acggcctcga agggcggccg cccgttgacg ccgtcgacgg tcgagatcga
    20221 gttggcgaag ccgatgtcgg ccttgccctg cgagaccgcc agaacattgg cgatgccggc
    20281 gcccggcagg acctggacgt tgatcccgct cttgccggcg atgttggcga tggcgccgcc
    20341 cagcggatac caggagccgc cctgcggccc ggtcatcagc ttgacctgct gtgcctcggc
    20401 cgtggtcgcg gcggtggtgg caaggcccag cccgagggcg aggatcgcca gcgaatgtcg
    20461 tgtcttcatg ctctgctccc tgatcgactg ggcgccgatg gcgcctttcc gttcgtcccc
    20521 aatgtgccgg acgagacggc tggtgcaagc ttcaattgcc gaggcgccgg ccgtgccagg
    20581 cgatgtggtc ggccatgaag ctcgccatga agtagtagga atggtcgtac tcggcgtgcc
    20641 ggcgcagctc gagcgggatg ccggccgtgg cgcaggcggc ttccaagagc tcgggcttga
    20701 gctggctctc gaggaagttg tcaccgagcc cctggtcgac caggatcccg gctgccttcg
    20761 caccgtcctc gatcagcgcg gtggcatcgt aggcgcgcca cttggtgcga tccaggccga
    20821 gatagccggc gagcgccttc tcgccccagg ggcagcgcat gggcgaggcg atgggggcga
    20881 aggcggagac cgagcgatag agcgccgggt tcttcagggc cagggtgagg gcaccatgac
    20941 cgcccatcga gtggccaaag atgccctgcc gggccgggtc gatcggcagc tcggcggcga
    21001 cgagatcctg cagctcccgg gtgaggtagc tctccatgcg gtagttccgg gcccagggct
    21061 cgaccgtcgc atcgaggtag aagccggcgc ccgagccgaa gtcatacgtg tcgtgctcgc
    21121 ccggcaggtc gaggccgcgg gggctggtgt cggggcagac gacgatcagc ccggcggcgg
    21181 cggcagggcc ctggaagccg gccttggtgg tggcgttctc ccaggtgcag gtgaggcccg
    21241 agagccacca gaggacgggg cagcgccgcc cctcctcggc cgctggcggc aggtagacgg
    21301 agaactccat gtccgtgccg gtggtggtgg aggcatggcg atagaccgcc tggacgccgc
    21361 catggcagcg gtgcgcggcg gtggggcgga gcgccatcag tagacgacca cgccgcggat
    21421 cgacttgccc tcgtgcatga ggtcgaagcc cttgttgatg tcgccgagcg gcatggtgtg
    21481 ggtgatcagg tcgtcgatgt tgatcttcct ctccatgtac cagtcgacga tcctcggcac
    21541 gtcggtgcgg cccctggcgc caccgaaggc ggtgccgcgc cagacccggc cggtgacgag
    21601 ctggaagggc cgggtcgaga tctcctggcc ggcgccggcg acgccgatga tgacgctctc
    21661 gccccatccc ttgtggcagc attcgagcgc ctggcgcatg gtcttgacgt tgccgatgca
    21721 ctcgaagctg tagtcggcgc cgcctctggt cagctcgacc aggtgagcga ccacgtcctt
    21781 gtcgcccagg tccttcgggt tgacgaaatg ggtcatgccg aacttgcggg cggtcgcctc
    21841 gcgggccggg ttgagatcga cgccgacgat catgtcggcc ccgacgaggc gggcaccctg
    21901 gatgacgttg aggccgatgc cgccgaggcc gaagacgacg acattggagc cgggctcgac
    21961 cttggcggtg ttgatcaccg ccccgatgcc cgtggtgacg ccgcagccga tgtagcagat
    22021 cttgtcgaag ggcgcgtcct tgcggacctt ggccaccgcg atctcgggca ggaccgtgaa
    22081 gttgctgaag gtcgagcagc ccatgtagtg gtggatgacg tccttgccga ggcgaaagcg
    22141 cgaggtgccg tccggcatca ggccctggcc ctgggtggtg cggatcgcct ggcagagatt
    22201 ggtcttggga ttcaggcagt actcgcattc ccggcattcc ggggtgtaga gcgggatgac
    22261 gtggtcgccg ggcacgaccg aggtcacgcc cgccccgacc tcgcgcacga tgccggcccc
    22321 ctcgtggccg aggatgctcg ggaagatgcc ttccgggtcg ccgccggagc gggtgaactc
    22381 gtcggtgtgg cagagcccgg tcgccatgat ctcgaccagc acctcgccgg ccctggggcc
    22441 ctcgagctcg accgtcgtca cctcgagcgg cttgcccgcc gcgaacgcca c
//
LOCUS       DAOTFI010000043        22474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2726_length_22474_cov_7.134083, whole genome shotgun sequence.
ACCESSION   DAOTFI010000043 DAOTFI010000000
VERSION     DAOTFI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22474
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2726_length_22474_cov_7.134083"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="P5558_06160"
     CDS             <1..1401
                     /locus_tag="P5558_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY23925.1"
                     /translation="AMPEQPDLDDLATLAAALDAPEDGEAGICLAGAPGPGENLAVIT
                     LFASALGDAKPTPEALDDPSGSWHAALLRYTDMLTAYGPPDAGAFGNAELAGRFAAGG
                     CAIWVGPSGALASLARDPGPGPTDWLGVALAPGGPRDGSIGWRRSTSFAVPVEAPNPA
                     EARDFIAWATSAAVLEKALPTAPPEPFAATLATALGRAVPAGFETLHGELARDPLPVV
                     DEIGQVLAAPLAGELAVDAALAVAAGLLDRLVPEPAMANGGGPAREVPSLVRASRLFA
                     GPGQYPPEAFAAYGILAFPSRATSADLERHQMLCEAYVANLPRTDELGLPVEQQMVTV
                     WPLTEDKIADELNRAPDSSPCETAVRNYGLPVALQAIRDVGRARLRPLGRGPLLLAWS
                     PATGKGDPEAIVLIADLSDVTTAEQAATLFALWRQDIEANPALWNRGWDIEALRTGIR
                     LWVDRVGSQIFAVLGA"
     gene            1405..1674
                     /locus_tag="P5558_06165"
     CDS             1405..1674
                     /locus_tag="P5558_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23926.1"
                     /translation="MGKWVAFVIQLGPAGIVAMLLALAAVWWVAPTTGGGTLLLLLIV
                     FAVAIVLLEALRKLARRLRRPATPAPEAEPEPEPAGDPADSPPHS"
     gene            complement(1763..2194)
                     /locus_tag="P5558_06170"
     CDS             complement(1763..2194)
                     /locus_tag="P5558_06170"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRY23927.1"
                     /translation="MQLALEAAGRAAALGEVPVGAVLVDPTGRVLATAHNLVERDHDP
                     TAHAEILAIRAAARSLGSPRLVDCDLYVTLEPCPMCAQAIAFARLRRLYFGAWDPKGG
                     GVENGPRIFQSSSCHHRPEVYGGISEAAAADLLRGFFQDRR"
     gene            complement(2224..2898)
                     /locus_tag="P5558_06175"
     CDS             complement(2224..2898)
                     /locus_tag="P5558_06175"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRY23928.1"
                     /translation="MIITCPSCATRYTIPPESLGADGRMVRCTACGHRWFVGHEPPAP
                     AEPEAPPPPLDEAPALPPAGEARPGQKPGSSTGATVGWLGVLLLLLLLTGLVLGRNEV
                     VALVPEAAPIYQRMGLPVTREIGLELRGVVSERLDDTESGATGEGDTLRIAGDVVNVS
                     GAERSVPPVRIALLDAARDEVAVHLVEVPQDVLADGASARFELLLDDPPEAASNFSVT
                     FAVPEK"
     gene            3018..3731
                     /locus_tag="P5558_06180"
     CDS             3018..3731
                     /locus_tag="P5558_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY23929.1"
                     /translation="MTGAAATILQLDGVDLRYGDTPPVLHGLDLTLQAGSFHFLSGPS
                     GAGKTSLLRLISLSHPPSRGTVRVFGREIDKINREELATLRQRIGVVFQDFRLLDHLS
                     AFDNVALPLRIGGADEEQTARFVADMLGWLGLGDRLEARPAELSMGQRQLVCIARAVV
                     AKPRLLLADEPTSNIDAKNAERLMNLFIQMHRLGTAVVIATHSAELLRRYPYAVLHIA
                     QGRLTGPRPPAGAASSGTG"
     gene            3735..4637
                     /locus_tag="P5558_06185"
     CDS             3735..4637
                     /locus_tag="P5558_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23930.1"
                     /translation="MRNPDLDLPLETTAFGRFLLWLTAGLVFLCVLAFAVAAIADAQL
                     TALRQEPRIVTVALPPNPEPAAARREVDDVLAHVRALPGVAYASLIDEAAFGELVDEL
                     LVDDAADRATGGLFGARMPLPRLIDIAFNPGAAIDLPALDEDISTLVPGATLGDSGQI
                     ERSRERLAVLFRGVAGSFGLLLLAVIVVVVVWLTRAGLEAHRQTIDLLRQMGAGDRYL
                     ARQFEQHALAKALRGALLGFALAISVVIVVVYVPTLLGEAPLAAHELAPVHWVLLAMV
                     PVVAALLATLVARLTAHHGLARLR"
     gene            4686..5408
                     /locus_tag="P5558_06190"
     CDS             4686..5408
                     /locus_tag="P5558_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY23931.1"
                     /translation="MPVVRSLLFNIWFWAWTVLASLAILPFAAFVSAGQMRRYAAFWM
                     KGIHLGMRLLIGLGHRVQGLEHLPAGPCIIASKHQSAFETLAYHPIRPDLVIGLKEEL
                     TRIPVFGWYLRIARNISIDRGGAAKAMRSLVRGAEAAVADGLSILLFPEGHRQPPGAP
                     PDYKPGIAAVYKAANVPVVPVALDSGRFWPRRSFVKRPGVITLRFLEPIPPGLDRQTF
                     MRLLEERTEAACDELLAEPLQA"
     gene            complement(5399..5953)
                     /locus_tag="P5558_06195"
     CDS             complement(5399..5953)
                     /locus_tag="P5558_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRY23932.1"
                     /translation="MTAAPLPARVWFFAYGSLMWNPGFEPVASAPALLHGYHRSLCVY
                     SWVWRGTPEKPGLVLGLAPGRACFGRALAVEAAQEAETLGYLDGRELVTDVYERRRLE
                     LVLGDGRKVDAWCYLARPDHVQFAGDLPLAEVLRFVRQGRGRGGANPDYVLATVAHLR
                     KMAVRDPALEAVAEALEAETAGQA"
     gene            complement(5971..6888)
                     /locus_tag="P5558_06200"
     CDS             complement(5971..6888)
                     /locus_tag="P5558_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HRY23933.1"
                     /translation="MKPFSRVALIGIGLINGSLARVLQKEGLAGEIVAASRRQETLDR
                     ALELGLCDSATTDPAAAVRDADLVVLGTPPGSMGALAAAIGGSLGTDAIVTDVGSVKA
                     QIVQDVRPHLPAPQHFVPGHPVAGTEHSGPDAAFASLFRQRRCILTPLADADPASVER
                     VRELWEAAGSLVDVMTPEHHDKVLAITSHLPHLIAYTIVGTVADLEDQARTEVFKYAA
                     GGFTDFTRIAGSDPTMWRDVFLNNREAVLEMLGRFTEDLVALQRAIRWGEGEALFDLF
                     TRTRAIRRGVVEAGQAYVRQPEDPLATKP"
     gene            complement(6885..7991)
                     /gene="hisC"
                     /locus_tag="P5558_06205"
     CDS             complement(6885..7991)
                     /gene="hisC"
                     /locus_tag="P5558_06205"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRY23934.1"
                     /translation="MASTAPRPRPGILEIQAYQGGRAETGSGRPAAKLSANESAIGPS
                     PLAVEAFRGTAERLHRYPEGGSEALRAALARRHNLDPARLVCGAGSDELIALLARAYA
                     GPGEEVLFTEHGFLMYRLSALGVGATPVAAPEQRLRADVDALLARLTSRTRIVFIANP
                     NNPTGSYLSGDELRRLHAGLPADVLLVVDAAYAEFVEAADYDSGQELAMSEPNVVMTR
                     TFSKLYGLAALRLGWLYGPPEVVDVINRLRGPFNVALPAQEAGIAALEDVAHEQRARA
                     HNAKWLPWLEAELEGLGLHVHPSVGNFLLVEFPAEPGRNAVAANAHLEADGLIPRQMA
                     AYGLPLCLRITVGREAENRALVASLGRFTKGEPR"
     gene            complement(8038..8922)
                     /locus_tag="P5558_06210"
     CDS             complement(8038..8922)
                     /locus_tag="P5558_06210"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRY23935.1"
                     /translation="MAEPAPDELSRLRAEIDRLDDAVVDLLLERIAVVRRIAEVKTNA
                     TGLALRPSREADLIRRLTARAAPALPPAALTRMWRELLAATTRLQTPFKVAVLDDPAQ
                     PHVTTLARDQFGTTTPLTMVETAYQGFRLIANGEAELLVVPAPTEDSYWWRQVTPTLI
                     DSPLRVVGRLPFCPADTDDAAPVPGALVLGTLLAEPSSADLTLLAIETDLNLSRARLR
                     DLIAAAGVDLVSLVALRDLQPESAFFLAEVAGMGEVVQAGLGQAFAPLRERLLRLDVL
                     GSYPRPLGVIADSSDSDA"
     regulatory      9039..9117
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            9185..10138
                     /gene="metA"
                     /locus_tag="P5558_06215"
     CDS             9185..10138
                     /gene="metA"
                     /locus_tag="P5558_06215"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRY23936.1"
                     /translation="MPIKIPNDLPAAAMLATEGVRLIGENEALRQDVRPLQIALLNLM
                     PEKPKTETQLARLLGATALQVELTLLTTSTYRPQNVPLSHLQSFYRQWDEVRDRKFDG
                     LIVTGAPVELLPFEEVKYWPELAAIFDWSRTNVHSCFHICWGAQAALFHFHGVPKYTL
                     PVKRFGIYTHYVIERRDAARPGLLRGFDDYFLVPVSRHTEVRRADIEAVPGLEVLAVS
                     NAAGVCLVHDPRHREVFMFNHLEYDTFTLADEYRRDCAGRGDVSLPLNYYPDDDPTKA
                     PRNCWRAYAHLLFGNWINEIYQTTPFDSSLIGLSEAAVRAA"
     gene            complement(10110..10943)
                     /locus_tag="P5558_06220"
     CDS             complement(10110..10943)
                     /locus_tag="P5558_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY23937.1"
                     /translation="MRPDIVELTEFYASRDGEYARRLINHQLRQIWPDLGGMELLGIG
                     FATPFLATFEEAGRRVALMPATQGVAAWPKPDRNLAVLGREDELPFDDRTFDRLILAH
                     ALECSPHGNRLMREAWRVLRDGGRMVLIVPNRRGLWCWSEKTPFGFGQPFSKGQLERS
                     LKNHLFATGELRGALFMPPLARVLPRGRRAVRRLSVSVERLGLRLLPHFSGVLIGEAV
                     KDVYAPTPEFAAIRPSSRRYVPVPEAVTAREQPCQRTAAGRPAARVVGPGLTPPARPL
                     R"
     gene            11033..11758
                     /gene="gloB"
                     /locus_tag="P5558_06225"
     CDS             11033..11758
                     /gene="gloB"
                     /locus_tag="P5558_06225"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010669194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRY23938.1"
                     /translation="MPLEIELLPILSDNYCYLLHEPASGLSGVVDPAVEEPVAARLAE
                     LGRGLDWILTTHHHADHTGGNLALKERYGCRIAGPAAEAAKVPGIDLQLAHGDCFAFG
                     EETATIIATPGHTAGHISYYFAASDALFCGDTLFALGCGRLFEGSAETMWKSLGRLMA
                     LPDRTRVYCGHEYTQSNARFALHVDHENAALVARVAKIDELRQAGEPTIPSTIGLERA
                     TNPFVRAGDAAEFGRLRALKDQS"
     gene            complement(11742..12461)
                     /locus_tag="P5558_06230"
     CDS             complement(11742..12461)
                     /locus_tag="P5558_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRY23939.1"
                     /translation="MMLTRENLQNGWLDRMTRNARAAGDTTRIRTEAELAACRASALA
                     GHAPGQDLWLFAYGSLIWNPAFHFAERRQGCIWGYHRRFCLWTNLGRGTVQRPGLMLG
                     LDRGGSCRGVLYRIAADLVEEELTLVWRREMITAAYRPTWVQGRTSSGPVQALTFTIN
                     RAHPRYAGKLGEERVVETLATAAGPLGPCCDYLFNTHRHLAELAIHDPQLGRLCAAVR
                     DRQAAAPAQLANSMAPATTGP"
     gene            12566..13192
                     /gene="plsY"
                     /locus_tag="P5558_06235"
     CDS             12566..13192
                     /gene="plsY"
                     /locus_tag="P5558_06235"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRY23940.1"
                     /translation="MIDPLGGPAYWLPFLVALVAGYLAGSIPFGLLFARASGAGDIRK
                     IGSGNIGATNVLRTGRKGLAAATLVADIAKGWLPTFAAWHWLGPDVAVATGLGTVLGH
                     CFPIWLRFRGGKGVATAAGVILALTPVVLPIVLAVFIVLVWATRYVSLGSCIAALAAP
                     VTAWLIGHVQAAELYALLALVIVVLHRANIVRLLKGEESKLSFAAKSS"
     gene            complement(13167..13811)
                     /locus_tag="P5558_06240"
     CDS             complement(13167..13811)
                     /locus_tag="P5558_06240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY23941.1"
                     /translation="MAEKSIEDRNWLQFYRATAGRPPRPTLLRALSARTASRRPVGRR
                     AIDLGCGTGRDTLPLLATGWHVLAIDPEPVALRTLEERTPLAWRPRLTTRLARLEATS
                     LPAAELVNASFSLIFAARPPLLAAHLRCIHAALVPGGRFAGQLLGPRDSWALAGQAVG
                     VDRCRLAHLLAGFELEHLVVEETDAVTPKGEAKHWHIWHVNARKAVVRSSSRRS"
     gene            13851..15041
                     /gene="dprA"
                     /locus_tag="P5558_06245"
     CDS             13851..15041
                     /gene="dprA"
                     /locus_tag="P5558_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRY23942.1"
                     /translation="MAAAPILAERTEIPASGWSDERRAWLRLARSRGVGATLFCRLIE
                     RFGKAEAALAALPRLRAKGELAGVDLLAEAAVEEEIEALEGLGGELLLGCDPAYPAAL
                     AAIADPPPVLALQGRMELLAARPLAIVGARNASGNGRFLARALAREASQRDLVIVSGL
                     ARGIDTAAHEGGLEGPGSTIAVLGSGIDIAYPPENAELLARIATEGLVMSERPIGTPP
                     LARHFPRRNRIIAGLASAVLVVEAAARSGSLMTARLAAAEGREVMAVPGSPLDPRHAG
                     TNQLLRDGAALVTSIDDVLEALPAWSRPQAPVGPPSPVAPRPQRNAAKPRPAPQPECP
                     APQAPPLPDRIAMLVGVEPMPVDEVIRQCHAKPAEVQQALLDLELDGRVRRHPGNRIS
                     RALA"
     gene            15176..17824
                     /gene="topA"
                     /locus_tag="P5558_06250"
     CDS             15176..17824
                     /gene="topA"
                     /locus_tag="P5558_06250"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRY23943.1"
                     /translation="MDVVVVESPTKAKTIRKYLADAPDVEASYGHVRDLPSQDGAVLP
                     DDDFRMVWEVLPGRKKNVDEIVRAVKKARRLFLATDPDREGEAISWHLLEVLKERGAL
                     KGIEVKRVVFHEITKRAVTEAMAHPRDLDQSLIDAYLARRALDYLVGFTLSPVLWRKL
                     QGSRSAGRVQSVALRLICEREAEIEAFTAREYWTIEALLETPDQTRFKARLTHLAGKK
                     LDKFALGDKVAAEAALAKVQAATLAIGPVEKKQVSRNPSPPFSTSTLQQEAARKLGFS
                     ADRTMKTAQRLFEGTEIGGETVGLITYMRTDSVQLSRDAISAARKRIAQSFGDAYLPE
                     QPRIFKTKTKNAQEAHEAIRPTDVGREPKTVARFLDNDQSRLYELIWKRTMASQMAAA
                     RLDRVTVDVLDEKGAVQLRATGQTIAFDGFLKLYQEGRDDPLGDDEDGEGVLLPALVT
                     GTKVAERGVTPEQHFTEPPPRFSEASLVKRLEELGVGRPSTYASIISVLQDRDYVRLE
                     QKRFVPEDRGRLVTAFLTSFFDRYVQPTFTAALEDQLDRVAAGELGWKDVLRAFWEPF
                     VGSIGEVSELRVAQVIDALNEALSAKLFPPRTDGGDPRICPLCGNGQLSLKLGRYGAF
                     IGCSNHPECRYTRKLDDDPNGDPAARNDEKLLGVDPLTEEEVWLKNGRFGFYLQSGSG
                     DALRRSSLPPNLTPTEIDLQTALALLALPRIVGTHPETGAEITAGINRYGPFVQHQGK
                     FVRLEADDDVLSLGMNRALTLIAEGGSRGRQRAAAAPLRELGAHPDTGEKVVILDGRF
                     GPYVKHGKTNASLPKGRPVEELTMDEAVALLAAREAKGGSKGKGRTTSRKAPAKAKPA
                     KATAVKKKPAARKPAARKKSAAGGGS"
     gene            17828..19954
                     /locus_tag="P5558_06255"
     CDS             17828..19954
                     /locus_tag="P5558_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HRY23944.1"
                     /translation="MRRGRGHGDRGPRDRSTGRRNRFERTGTPPGMAAVEVTAIDDAG
                     ELVGESPAVPGAAIRVLDADGGEAPLEVGDRALMRLSTDAEGAIEGRLFRKLGRVARD
                     IVGVVEASGGTLFLRSSRRSADRDQPTRLKLEPGDLTLADGDMVRAELDRGRPLAAPR
                     ARAVERLGSSTDPAAIALSIAAELELPTVFGPEALAVAERATRPALGERTDLRDLSLV
                     TIDGADARDFDDAVWADPEPLADGGFRAVVAIADVAHYVRPGDPLDREALERGNSVYF
                     PDRVIPMLPEALSNGLCSLKPDEDRASIAVELFFDSGGVLKRWNFTRALIRSRARLTY
                     EQVQDAADGRPDALTAPLLESVIRPLYDVYEKLAAARRKRGTIELELPERRVLLDGEG
                     KVTDIVQRQRLASHMLVEELMIAANVAAARALADRKLPCLFRVHDKPDPVRLEALAQY
                     LERLSIGWSRTAKKPGDFTAMLERIVEPSLREQVSGFVLRAQAQALYQPDNLGHFGLN
                     LRHYAHFTSPIRRYSDLVVHRLLIHHEKLAGTGDGRPYDLEQLDRFGRLVSATERKAV
                     QAERQALARYTALYMADKEGAQFTGRVIGVQRFGLFVVLDGTGAEGLVPVSTLGDDAF
                     HFDEQHQALVGVRYGDTFGLGDAVTVTLADVDLVLGQLAFRIDSHERAHAAELARTAW
                     AKAGPMRRGRGSPNRPHRASGRRGKR"
     gene            19992..21125
                     /locus_tag="P5558_06260"
     CDS             19992..21125
                     /locus_tag="P5558_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY23945.1"
                     /translation="MVSAAVVGTGVALSFPLVALVLEAEGATATAIGLNAAAHGLGVL
                     LAAPFFGRTVRKLGAARGMRVGLTLAAILLLGFPLATDLGYWFILRLGFGAATALVFV
                     ISEAGINALAEDGRRGRVLAMYGTMFGVGYALGPLILGLLGSDGALPFLVGAAVLAAG
                     ILPTFAVPSIDGALDGRPGAYAARRLMRLGRAIPLVLLVALVVGWTETSQFSLLPVWA
                     IRHGLDETTVAWLLGLWVAGDILFQYPIGWLADRLPRRRLLLAAALGATLLALTLTTP
                     LVAATPWLLIPALIAHGGLIGGSYVLALTLMGERFRGAALVGANAAFVLAFEIGAMIG
                     PPLTGLAFGAIGTAGLPLALALVTATIVAAGLHARRASALSRA"
     gene            21315..21845
                     /locus_tag="P5558_06265"
     CDS             21315..21845
                     /locus_tag="P5558_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23946.1"
                     /translation="MTRVLRTRRALLLAGAASAGAAFLGAGASGLLAPVRAQGLAATP
                     TMRGGANNYRPGAPLVERIGHGGFWMTGTVRRAGDGAPLPGVRIQIWAHTTEGHERDP
                     HSHGATLTDADGVFRMDMPQIVPAFGQPHGHLAYDPEFDGREHDGRGFETVFLRPVMA
                     SASETSLSADFVLQPA"
     gene            21850..22461
                     /locus_tag="P5558_06270"
     CDS             21850..22461
                     /locus_tag="P5558_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRY23947.1"
                     /translation="MVPVRAILIWAALAAALVVPIAVAATSPLLAWRQPIYIAAGFAG
                     IVAMALLLLQPLLAGGYLPGLPLRRSRRLHAWIGAGLVAAVVVHVAGLWLTSPPDVID
                     ALLFRSPTAFSAWGVIAMWAVFANALLTASRRRLRLRSTVWRFWHTALAAVIVPTSVV
                     HALLIEGTMGTLSKAAICALVLAAAVKVIVDRRSWTLLSRQKV"
BASE COUNT         3160 a         8479 c         7638 g         3197 t
ORIGIN      
        1 gccatgcccg agcagcccga cctcgacgat ctggcgacac tggcggcggc gctcgatgcg
       61 ccggaagacg gcgaggccgg catttgcctc gccggagcgc ccggcccggg cgagaatctc
      121 gccgtcatca cgctcttcgc cagcgcactc ggcgacgcca agcccacgcc agaggccctg
      181 gacgacccga gcggcagctg gcacgccgcc cttctccgct ataccgacat gctcaccgcc
      241 tacggcccac cggatgccgg cgcctttggc aacgccgaac tcgccggtcg cttcgccgcc
      301 ggcggctgcg ccatctgggt cggcccgagc ggggcgctgg catcgctggc ccgggatccc
      361 ggccccggtc cgacggactg gctcggggtc gccctcgcac ccggcggccc gcgcgacggc
      421 agcatcggct ggcgccgctc aaccagcttc gccgtgccgg tcgaggcccc gaatccggcg
      481 gaagcccggg atttcatcgc ctgggcgacg tccgccgccg tgctcgaaaa ggccctgccg
      541 acggcacccc ccgagccctt cgccgccacg ctggctacgg ccctcggccg ggccgtgccc
      601 gccggcttcg agacgctgca tggcgagctc gcccgggatc ccctgccggt ggtcgacgag
      661 atcggccagg tgctcgccgc cccgctcgcc ggcgagcttg ctgtcgacgc ggcccttgcg
      721 gtggcggccg gcctgctcga ccgcctcgtg cccgagccgg ccatggccaa tggcggcggg
      781 cccgcgcgcg aggtgccctc cctggtccgc gcctcccggc tcttcgccgg ccccggccag
      841 tatccgcccg aggcgttcgc ggcctacggc atcctggcgt tcccgtcccg tgccacgagc
      901 gccgacctcg agcgccacca gatgctgtgc gaggcctatg tcgccaacct gccgcggacg
      961 gacgagctcg gcctgccggt cgagcagcag atggttaccg tctggccgct gaccgaggac
     1021 aagatcgcgg acgagctcaa tcgtgccccg gacagctcgc cctgcgagac ggcggtccgc
     1081 aattacggcc tgccggtggc gctgcaggcg atccgcgatg tcggccgggc gcggctccgc
     1141 ccgctcgggc gcggccccct cctgctcgcc tggtcgcccg ccacgggcaa gggcgacccc
     1201 gaggcgatcg tcctgatcgc cgacctctcg gacgtcacca cggccgagca ggccgccacc
     1261 ctcttcgccc tctggcggca ggacatcgag gccaatcccg cgctctggaa ccggggctgg
     1321 gacatcgagg cgctgcgcac cggcatccgc ctctgggtcg accgcgtcgg ctcgcagatc
     1381 ttcgcggtcc tcggggcctg aggcatgggc aagtgggtcg ccttcgtcat ccagctcggc
     1441 cccgccggca tcgtcgccat gctgctggcg ctggccgccg tctggtgggt ggcgccgacc
     1501 accggcggcg gcacgctcct gctcctgctc atcgtcttcg ccgtggccat cgtgctgctc
     1561 gaggccctgc gcaagctggc ccgccgcctc cggcggcctg cgacaccggc gcccgaggca
     1621 gagccggaac ccgaaccggc cggcgacccg gcggattcac cgccccactc ctgagctcgg
     1681 gccgggtggc cgaagtcgat ggagccgatg cagcggcccg ggtcggcagc cgcaaggcca
     1741 ccgacagccg ggatgccggc cttcagcgcc ggtcctggaa gaaacccctg agcaggtcgg
     1801 ccgcggccgc ctcggagatc ccgccataga cctcgggacg atggtggcag gacgaactct
     1861 ggaagatgcg cggcccgttc tcgaccccgc cgcccttcgg atcccaggcg ccgaaataga
     1921 gccggcggag tcgggcgaag gcgatcgcct gggcgcacat cgggcagggc tcgagcgtga
     1981 catagagatc gcagtcgacg agccgcgggc tgcccaggct ccgggccgcg gcgcgaatcg
     2041 ccaggatctc ggcatgggcg gtcggatcgt ggtcacgctc gacgagattg tgggcggtcg
     2101 ccagcacgcg ccccgtcggg tccaccagca cagcacccac cggcacctcg cccagcgccg
     2161 cggcccggcc ggcggcctcg agcgcgagct gcatgggccg acctgccgcc ggaccggggt
     2221 cggtcatttc tccggcacgg cgaaggtcac gctgaaattg ctcgccgcct cgggcggatc
     2281 gtcgagcagg agctcgaagc gggccgaggc gccatccgcc agcacatcct gcggcacctc
     2341 gaccagatgg acggcgacct cgtcccgcgc ggcgtcgagg agcgcgatgc gcaccggcgg
     2401 gacgctgcgc tcggcacccg agacattgac cacgtcgccg gcgatccgca gcgtgtcgcc
     2461 ctcgccggtg gcgccggatt ccgtgtcgtc gagccgctcg gagacgacgc cgcgcagctc
     2521 gaggccgatc tcgcgggtga cgggcaggcc cattcgctgg tagatgggag cggcctccgg
     2581 cacgagggcg acgacctcgt tgcggccgag gacgagaccg gtgagaagca ggagaagcag
     2641 cagcacgccg agccagccca cggtcgcccc ggtcgagctg ccgggcttct gcccgggccg
     2701 cgcctcgcct gccggcggca gcgccggtgc ctcgtcgagc ggcggtggcg gcgcctccgg
     2761 ctcggccggg gccggcggct cgtgaccgac gaaccagcga tgcccgcaag ccgtgcaacg
     2821 gaccatgcgg ccgtcggcgc cgaggctttc gggcgggatg gtgtagcgcg tggcgcagga
     2881 aggacaggtg atgatcatcg ctcagcgctt ggcagcccgg gatgtccgcc atataacgca
     2941 tgagttgagg cggttgtgaa gctattaaac aaatcatggt tggctgccac gcccgcgaac
     3001 ctgctgacgg agtggacttg accggcgctg ctgccaccat cctgcaactc gacggcgtcg
     3061 acttgcgcta tggcgatacg cctcccgtcc tgcatggtct cgacctcacg ctccaggctg
     3121 gcagcttcca cttcctcagc ggcccctccg gtgccggcaa gacctcgctt ctgcgcctga
     3181 tcagcctgtc gcatccgccg tcccggggca cggtccgtgt tttcggccgg gagatcgaca
     3241 agatcaaccg cgaggagctc gccacactgc gccagcgcat cggcgtggtg ttccaggatt
     3301 tccgcctgct cgaccatctc tccgccttcg acaacgtcgc cctgccgctc aggatcggcg
     3361 gcgccgacga ggagcagacg gcgcgtttcg tcgccgacat gctcggctgg ctcgggctcg
     3421 gcgaccggct ggaggcgagg ccggccgagc tctcgatggg ccagcgccag ctcgtctgca
     3481 tcgcccgcgc cgtggtcgcc aagccccgcc tgctgctcgc cgacgagccg acgagcaata
     3541 tcgacgcgaa aaatgccgag cggctcatga acctcttcat ccagatgcac cggctcggca
     3601 ccgccgtggt gatcgccacg cacagcgccg agctcctgcg ccgctatccc tatgcggtgc
     3661 tgcacatcgc ccagggccga ctgaccggcc cccgcccgcc ggccggcgca gcttcgagcg
     3721 gcaccggctg agcgatgcgc aaccccgatc tcgacctgcc gctcgagacc acggccttcg
     3781 gtcgcttcct gctctggctg acggccgggc tggtcttcct ctgcgtcctc gccttcgccg
     3841 tcgccgccat cgccgatgcc cagctcacgg cccttcgcca agagccgcgc atcgtcaccg
     3901 tggccctgcc gccgaacccc gagcccgcgg cggcccgccg cgaggtggac gacgtgctgg
     3961 cgcatgtccg ggccctgccg ggcgtcgcct atgccagcct gatcgacgag gcggccttcg
     4021 gcgagctggt cgacgagctc ctcgtcgacg acgctgccga tcgggcgacc ggcggcctct
     4081 tcggcgcccg catgccgctg ccgcggctca tcgacatcgc cttcaacccc ggtgccgcga
     4141 tcgacctccc tgccctcgac gaggacatct ccacgctcgt gcccggcgcc acgctcggcg
     4201 attccgggca gatcgagcgc agccgggaac ggctcgccgt gctcttccgc ggcgtggcgg
     4261 gctccttcgg cctcctgctg ctcgccgtca tcgtcgttgt cgtcgtctgg ctcacccggg
     4321 ccgggctcga ggcgcaccgg cagaccatcg acctcctgcg gcagatgggg gcgggcgacc
     4381 gctacctcgc ccgccagttc gagcagcatg ccctggccaa ggcgctccgg ggcgcgcttc
     4441 tcggcttcgc cctcgccatc tcggtcgtca tcgtcgtggt ctacgtgccg accctcctcg
     4501 gcgaagcacc gctcgccgcc cacgagctgg ccccggtcca ctgggtgctg ctcgccatgg
     4561 tgccggtggt ggcggctctc ctcgccacgc tcgtcgcccg gctcaccgcc caccacgggc
     4621 tggcgcggct ccgctgagcg tgcgatgggg gacctgcgcc ccacgagact gctggagcca
     4681 gccccttgcc ggtcgtccgc tcgctgctct tcaatatctg gttctgggcc tggaccgtcc
     4741 tcgccagcct ggcgatcctg cccttcgccg ccttcgtctc ggccggccag atgcggcgct
     4801 acgcggcctt ctggatgaag ggcatccatc tgggcatgcg gctgctgatc gggctcggcc
     4861 atcgcgtcca gggtctcgag catctgccgg cgggcccctg catcatcgcc agcaagcacc
     4921 agtcggcctt cgagacgctg gcctatcacc ccatccggcc ggatctggtg atcggcctca
     4981 aggaggagct gacccgcatc ccggtcttcg gctggtatct caggatcgcg aggaacatct
     5041 cgatcgaccg gggcggcgcc gccaaggcca tgcgctcgct ggtgcgcggt gcggaggcgg
     5101 ccgtcgccga cggcctgtcg atcctgctct ttcccgaagg ccaccgccag ccgccgggcg
     5161 ccccacccga ctacaagccc gggatcgccg cggtctacaa ggcggccaac gtgccggtgg
     5221 tgccggtagc cctggattcg ggccgcttct ggccgcggcg cagcttcgtc aagcggcccg
     5281 gcgtgatcac gctgcgcttc ctcgagccga tcccgccggg cctcgaccgg cagaccttca
     5341 tgcgcctcct cgaggagcgc accgaggcgg cctgcgacga gctcctggcg gagccccttc
     5401 aggcctgacc ggcggtctcc gcctcgagcg cttccgcgac cgcctcgagc gccggatccc
     5461 tgaccgccat cttgcgcaga tgggcaacgg tggcgagcac gtagtcgggg ttcgccccgc
     5521 ctctgccccg gccctgccgg acgaagcgca gcacctcggc gagcggcagg tcgcccgcga
     5581 actgcacgtg gtccggccgg gccagatagc accaggcatc gaccttgcgc ccgtcgccga
     5641 gcacgagctc gaggcgtcgc cgctcgtaga catcggtgac cagctcacgg ccgtcgagat
     5701 agcccagcgt ctcggcctcc tgggccgcct cgaccgccag cgcccggccg aagcaggccc
     5761 ggcccggggc gaggccgagc acgaggcccg gcttctccgg cgtgccgcgc cagacccaag
     5821 agtagacgca gaggctgcga tggtagccgt gcagcagcgc cggggccgac gcgacaggct
     5881 cgaagcccgg gttccacatc agcgagccat aggcgaagaa ccacacccgc gccggcaagg
     5941 gggccgcggt catctcagcg ccttcccatc tcagggcttg gtcgcgagcg ggtcctcggg
     6001 ctggcggaca taggcctggc ccgcctcgac cacgccgcgg cggatcgccc gggtgcgggt
     6061 gaagagatcg aacagcgcct cgccctcgcc ccagcggatc gcccgctgca gggccaccag
     6121 atcctcggtg aagcggccga gcatctcgag caccgcctcc cggttgttga ggaagacgtc
     6181 gcgccacatg gtgggatcgg agccggcgat gcgggtgaag tcggtgaagc cgccggcggc
     6241 gtacttgaag acctcggtgc gcgcctgatc ctcgaggtcg gccaccgtgc cgacgatggt
     6301 gtaggcgatc aggtgcggca aatgcgaggt gatggcgagc accttgtcgt ggtgctcggg
     6361 cgtcatcacg tcgaccagcg agccggccgc ctcccagagt tccctgaccc gctcgaccga
     6421 ggccgggtcg gcatcggcga gcggtgtgag gatgcagcgg cgctgccgga agagcgaggc
     6481 gaaggcggca tccgggccgg aatgctcggt gccggcgacc ggatggccgg ggacgaaatg
     6541 ctgcggcgcc ggcagatgcg ggcggacatc ctggacgatc tgcgccttga ccgagccgac
     6601 atccgtgacg atggcgtccg tccccaggct gccgccgatc gccgccgcga gcgcgcccat
     6661 gctgccgggc ggcgtgccca gcacaacgag atcggcatct cgcaccgccg ccgccgggtc
     6721 ggtcgtggcc gaatcgcaga gcccgagctc gagggcgcga tccagcgtct cctggcgccg
     6781 cgacgccgcc acgatctcgc cggccagccc ctccttctgc agcacccggg cgagcgagcc
     6841 gttgatcagg ccgatgccga tcagcgccac acgcgagaag ggcttcatct cggctcaccc
     6901 ttggtgaacc gcccgagcga ggcgaccagc gcccggttct cggcctcgcg cccgaccgtg
     6961 atccgcaggc agagcggcag gccataggcc gccatctggc gcggaatcag gccgtccgcc
     7021 tcgagatggg catttgcggc cacggcattg cggccgggct cggccgggaa ctcgacgagc
     7081 aggaaattgc cgacgctcgg atggacatgg aggccgagcc cctcgagctc ggcctcgagc
     7141 cagggcagcc acttcgcatt gtgggcgcgg gcccgctgct cgtgggcgac gtcctcgagc
     7201 gccgcgatcc ccgcctcctg cgccggcagc gccacgttga aggggccgcg cagccggttg
     7261 atcacatcga ccacttcggg cgggccatag agccagccga ggcgcagggc ggcgaggccg
     7321 tagagcttgg agaaggtccg ggtcatgacg acgttgggct cgctcatggc gagctcctgg
     7381 ccgctgtcgt aatccgccgc ctcgacgaac tcggcatacg ccgcgtcgac gacgagcaga
     7441 acgtccgccg gcaggccggc atgcaggcgg cgaagctcgt cgccagagag atagctgccg
     7501 gtcgggttgt tggggttggc gatgaagacg atgcgcgtgc ggctggtgag ccgggcgagc
     7561 agcgcgtcga catcggccct gagccgctgc tcgggggccg ccaccggcgt ggcgccgacg
     7621 ccgagggccg agagccggta catgagaaag ccgtgctcgg tgaacagcac ctcctcgccc
     7681 ggcccggcat aggcgcgggc caggagggcg atcagctcgt cggagcctgc cccgcacacc
     7741 agccgcgccg gatcgaggtt gtggcgccgg gccagggccg cgcgcagcgc ctccgagccg
     7801 ccctcgggat agcgatgcag ccgctcggcc gtgccgcgaa aggcctcgac cgcgagcggg
     7861 ctcgggccga tggcgctctc gttggccgaa agcttggccg ccggccggcc gctgccggtc
     7921 tcggccctgc cgccctggta ggcctggatc tcgagaatgc cgggccgcgg ccgcggcgct
     7981 gtgcttgcca tcacgcaata acccgcgatt ggaggaggta ggctgacgtc cagccgctca
     8041 ggcgtcgctg tcggaagaat cggcgatgac gccgaggggg cgtggatagc tgccgagcac
     8101 atcgaggcgc aagagcctct cccgcaacgg cgcgaaggcc tggccgaggc cggcctgcac
     8161 cacctcgccc atgcccgcca cctcggcgag gaagaaggcg ctctccggct gcagatcgcg
     8221 caaggccacg agcgagacga ggtcgacgcc agccgcggcg atcaggtcgc gcagccgggc
     8281 ccggctgaga ttgaggtcgg tctcgatggc cagaagcgtc aggtcggcgc tcgacggctc
     8341 ggccaggagc gtgcccagca ccagcgcacc cggcacgggc gcggcatcat ccgtgtcggc
     8401 cgggcagaac ggcaggcggc cgaccacgcg cagggggctg tcgatcaggg tcggcgtgac
     8461 ctgccgccac cagtagctgt cctcggtcgg tgccggcacg acgagcagct cggcctcgcc
     8521 gttggcgatc aggcgaaagc cctgatacgc cgtctctacc atggtgagcg gcgtcgtcgt
     8581 gccgaactgg tcgcgcgcca gggtggtgac gtgcggctgg gccggatcgt ccagcacggc
     8641 caccttgaag ggggtctgca gccgcgtcgt ggcggcgagc agctcgcgcc acatccgggt
     8701 gagcgccgcc ggcggcaggg ccggggcggc ccgcgcggtc agccggcgga tgaggtcggc
     8761 ctcgcgcgac gggcgcagcg ccaggcccgt ggcgttggtc ttgacctcgg cgatgcgccg
     8821 cacgacggcg atgcgctcga ggaggagatc gaccaccgcg tcgtcgaggc ggtcgatctc
     8881 ggcccgaagc cgactgagct cgtctggtgc aggttcggcc aagggtcgct tctcgcaggc
     8941 tcgcgctgga tttcaaggcg ctcacttacg ccgcggcgtc ggacgcgtaa agccacagcc
     9001 gcagccggca cccttgacag ccggtccgtg ccggccaaga tggcgtccgt ggtgatttga
     9061 gctgaccggc ttgcggccac gttaaacaag cagctaaaag gtcgtgcttg gcaggtctcc
     9121 tggccgcgcg gtcccgctag ccgacgttga cgtgggcgta ccggcggtgt gctggagaca
     9181 tctcgtgccg atcaagattc ccaatgatct gcccgccgcg gcgatgctgg cgaccgaggg
     9241 ggtgcgactg atcggcgaga acgaggcgct gcgccaggac gtgcggccgc tgcagatcgc
     9301 gctgctcaac ctgatgccgg agaagcccaa gaccgagacg cagctcgccc ggctgctcgg
     9361 cgccaccgcc ctgcaggtgg agctgaccct gctcaccacc tcgacctacc ggccgcagaa
     9421 cgtgccgctc tcgcacctgc agagcttcta tcgccagtgg gacgaggtca gggaccgaaa
     9481 gttcgatggg ctgatcgtca ccggtgcccc ggtcgagctc ctgccgttcg aggaggtcaa
     9541 gtactggccc gagctcgccg ccatcttcga ctggtcaagg accaacgtgc attcctgctt
     9601 ccacatctgc tggggggcgc aggcggcact gttccacttc catggcgtgc ccaagtacac
     9661 cctgccggtg aagcgcttcg gcatctacac gcactatgtc atcgagcgac gggacgcggc
     9721 ccgcccgggc ctcctgcgcg gcttcgacga ctatttcctg gtgccggtct cgcgccatac
     9781 cgaggtgcgc cgcgccgaca tcgaggccgt gcccggcctc gaggtcctgg ccgtctccaa
     9841 cgcggccggc gtctgtctcg tccacgaccc gcgccaccgc gaggtcttca tgttcaacca
     9901 cctcgaatac gacaccttca ccctcgccga cgagtaccgg cgcgactgcg ccggccgtgg
     9961 cgacgtctcc ctgccgctca actactaccc ggacgacgac ccgaccaagg cgccacgcaa
    10021 ttgctggcgg gcctacgccc acctcctgtt cggcaactgg atcaacgaga tctaccagac
    10081 cacgccgttc gacagctcgc tgatcggcct cagcgaagcg gccgtgcggg cggcgtgaga
    10141 ccgggcccga ccacgcgcgc cgccggccgg ccggcggcgg tgcgctggca aggctgctcc
    10201 cgcgcggtca ccgcctccgg caccggcacg tagcgccggc tgctcggccg gatggcggcg
    10261 aattccggcg tcggcgcgta gacgtccttg accgcctcgc cgatcagcac acctgagaaa
    10321 tgcggcagga gcctgaggcc gagccgctcg accgaaacgc tcagccgccg cacggcgcgg
    10381 cggcccctgg gcaggacgcg cgccaagggc ggcatgaaaa gagcgccgcg cagctcgccc
    10441 gtggcaaaca ggtggttctt caggctgcgc tcgagctggc ctttcgagaa gggctggccg
    10501 aagccgaacg gtgtcttctc gctccagcac cagaggccgc gccggttggg cacgatcagc
    10561 accatgcggc caccgtcccg cagcacccgc caggcctcgc gcatcagccg gttgccatgc
    10621 gggctgcatt ccagcgcatg cgccaggatc aaccggtcga aggtgcgatc gtcgaacggc
    10681 agctcgtcct cgcgccccag cacggcgagg ttgcggtcgg gcttcggcca ggcggccacc
    10741 ccctgggtcg ccggcatcag cgccacccgc cggccggcct cctcgaaggt ggccaggaac
    10801 ggcgtggcga agccgatgcc gagcagctcc atgccgccga gatccggcca gatctggcgc
    10861 agctgatgat tgatgagacg gcgcgcatac tcgccgtccc ggctcgcata gaactctgtc
    10921 agctcgacaa tatcgggccg catcgcctgg ccagctccct tgtcatgact tgcactatag
    10981 gccatccgac ccgtaattga agcgcaacgc agggaaatca gaggaaaccg ccatgcccct
    11041 cgagatcgag ctcctgccga tcctcagcga caactactgc tacctgctgc acgagccggc
    11101 gagcggcctc tccggcgtcg tcgacccggc ggtcgaggaa ccggtcgccg cacggctggc
    11161 ggagctgggc cgcgggctcg actggatcct caccacccat caccacgccg accacaccgg
    11221 cggcaacctg gcgctgaagg agcgctacgg ctgccggatc gcggggccgg ccgccgaggc
    11281 ggccaaggtg ccgggcatcg atctccagct ggcgcacggc gactgcttcg cgttcggcga
    11341 ggagacggcg acgatcatcg ccacgccggg gcacacggcc ggccacatca gctactactt
    11401 cgcggccagc gacgcgctct tctgcggcga cacgctcttc gccctcggct gcggccgcct
    11461 cttcgagggg tcggccgaga cgatgtggaa atcgctcggc cggctgatgg cgctgcccga
    11521 ccggacccgg gtttactgcg gccacgaata cacccagagc aatgcccgct tcgccctcca
    11581 cgtcgatcac gagaacgcag cgctcgtcgc ccgggtggcg aagatcgacg agctgcgcca
    11641 ggcgggcgag cccacgatcc cgagcacgat cggcctggag cgcgccacca accccttcgt
    11701 ccgggccggc gacgccgccg agttcggtcg gctccgggcc ctcaaggacc agtcgtagcc
    11761 ggcgccatgg aattcgcgag ctgggccggt gccgccgcct gccggtcgcg caccgcggcg
    11821 cacaggcggc cgagctgcgg gtcgtgaatg gcgagctcgg ccagatgccg gtgcgtgttg
    11881 aagagatagt cgcagcaggg gccgagcggc ccggcggcgg tcgccagcgt ctcgaccaca
    11941 cgctcctcgc cgagcttgcc ggcatagcgc ggatgggcgc ggttgatggt gaaggtgagc
    12001 gcctgaaccg gcccggacga ggtccggccc tgcacccagg tcggccggta ggcagcggtg
    12061 atcatctcgc gccgccagac cagggtcagc tcctcctcga cgaggtcggc ggcgatccgg
    12121 tagagcacgc cccggcagct gccgccccgg tcgagcccga gcatcaggcc cggccgctgg
    12181 acggtgccgc ggccgagatt cgtccagagg cagaagcgcc ggtgatagcc ccagatgcag
    12241 ccctgccgcc gctcggcgaa gtggaaggcc gggttccaga tcagcgagcc ataggcgaag
    12301 agccagagat cctggcccgg cgcgtggccg gccagggcgc tcgcccggca ggccgcgagc
    12361 tcggcctcgg tgcggatccg cgtcgtgtcg ccggccgccc gggcgttccg ggtcatccgg
    12421 tcgagccagc cgttctgcag gttttctcgg gtcagcatca gccagacggt ttcccacgca
    12481 cgcgcaatga agatagactt tgcttcgaac ggcgcaagct tgcggacccc ggtccgcatg
    12541 gccggcgccc ggttgcgggg gcaccatgat cgatccgctc ggcggcccgg cctactggct
    12601 gccgttcctc gtcgcactcg tcgccggcta tctcgcgggc tccatcccgt tcgggctgct
    12661 cttcgccagg gccagcggcg cgggcgatat ccgcaagatc ggctccggca atatcggcgc
    12721 caccaacgtg ctccgcaccg gccgcaaggg cctcgcggca gcgacgctcg tcgccgacat
    12781 cgccaagggc tggctgccca ccttcgccgc ctggcactgg ctcggccccg acgtcgcggt
    12841 ggcgaccggg ctcggcaccg tcctcggcca ctgctttccc atctggctcc gctttcgcgg
    12901 cggcaagggg gtcgccaccg cggcgggcgt catcctggcc ctgacaccgg tggtcctgcc
    12961 gatcgtcctc gccgtcttca tcgtcctcgt ctgggccacc cgctacgtct cgctcggctc
    13021 ctgcatcgcc gcgctcgcgg ccccggtcac cgcctggctg atcggccacg tccaggcggc
    13081 cgagctctat gcgctgctgg cgctcgtcat cgtcgtcctc caccgcgcca acatcgtccg
    13141 tctcctgaag ggcgaggaga gcaagctcag cttcgccgcg aagagctcct gaccaccgcc
    13201 ttgcgggcgt tgacgtgcca gatgtgccag tgcttggcct cgcccttcgg cgtcaccgcg
    13261 tccgtctcct ccacgaccag gtgctccagc tcgaagccgg cgaggaggtg cgcgagccgg
    13321 caccggtcga cgccgaccgc ctgacccgcc agggcccagc tgtcgcgcgg gccgagaagc
    13381 tggccggcga aacgcccgcc cggcaccagg gcagcgtgga tgcagcggag atgagcggcg
    13441 aggagcggcg ggcgagcggc aaagatcagg ctgaagctgg cattgacgag ttccgccgcc
    13501 ggcagggacg tggcctcgag ccttgccagt cgcgtcgtca gccgtgggcg ccaggccaag
    13561 ggcgtgcgct cctcgagggt ccggagggcc accggctccg gatcgatcgc caggacgtgc
    13621 cagccggtgg cgagcagcgg cagggtatcg cgcccggtgc cgcagccgag atcgatcgcc
    13681 cgccgcccga ccggccgccg gctcgccgtc cgggcggaca gggcgcgcag cagggtcggc
    13741 cgtggcggcc ggccggcggt cgcccgatag aattgcagcc aatttctatc ttcgattgat
    13801 ttttccgcca tctcctgcct caattcgcga tcatcgaagt gccggagccc atggccgccg
    13861 cccccatcct cgccgaacga acggaaatcc ccgcctccgg ctggagcgac gagcgtcgcg
    13921 cctggctcag gctggcgcgg tcgcgcggcg tcggcgccac cctgttctgc cggctgatcg
    13981 agcgcttcgg caaggccgaa gcggcccttg ccgcgctgcc gcgtctccgg gcgaaaggcg
    14041 agctcgccgg tgtcgatctc ctggccgagg cggcggtcga ggaggagatc gaggcgctcg
    14101 aaggcctggg cggcgagctg ctgctcggct gcgatccggc ctaccccgcg gcgctcgcgg
    14161 ccatcgccga cccgccgccg gtgctggccc tgcaggggcg gatggagctg ctggccgcgc
    14221 gccccctcgc catcgtcggc gcccgcaacg ccagcggcaa cggccggttc ctcgcccgcg
    14281 ccctcgcccg cgaggccagc cagcgggatc tcgtgatcgt ctccggcctc gcccgcggca
    14341 tcgacaccgc cgcccacgag ggcgggctcg aaggcccggg ctcgaccatc gccgtgctcg
    14401 gcagcggcat cgacatcgcc tatccgccgg agaacgccga gcttctggcc cggatcgcga
    14461 ccgagggcct cgtcatgagc gagcggccga tcggcacgcc acccctcgcc cggcatttcc
    14521 cgcgccgcaa ccggatcatc gccggcctcg cctcggccgt cctcgtggtc gaggccgccg
    14581 cccgctcggg ctctctgatg accgcccgcc tggctgctgc cgagggccgg gaggtcatgg
    14641 cggtgccggg ctcgccgctc gacccgcgcc atgccggcac caaccagctc ctgcgcgacg
    14701 gtgcggcgct cgtcacctcg atcgacgacg tgctggaggc cctgccggcc tggagccggc
    14761 cgcaggcacc ggtcgggccg ccatcccctg tggcgccgag gccacagcgc aacgcagcaa
    14821 aaccgcgacc cgccccgcaa ccggaatgcc cggcgccgca ggcgccgccg ctccccgacc
    14881 ggatcgccat gctggtcggg gtcgagccga tgccggttga cgaagtgata cgccaatgcc
    14941 atgcaaagcc ggcagaggtg cagcaggcgc tgctcgacct ggagctcgac ggccgcgtgc
    15001 ggcgccatcc cggcaaccgg atttcaaggg cgctggcctg atcttgacgg acaggagagc
    15061 tgccggccgg gcttgaaggt caggaatatc gaccgacctc tggcctcgac cgccgccgcg
    15121 gtccacggac gaaccaggcc ccgatttcat ggccgaggat ggattgaacc cgcgcatgga
    15181 tgtcgtcgtc gtcgaatcac cgaccaaggc caagaccatc cgcaaatacc tggcggatgc
    15241 gcccgacgtc gaggccagct acggccatgt ccgcgacctc ccgagccagg atggcgcggt
    15301 tcttccggac gacgacttcc gcatggtctg ggaagtcctc cccggcagga agaagaacgt
    15361 cgacgagatc gtcagggcgg tgaagaaagc ccgccggctc ttcctcgcca ccgaccccga
    15421 ccgcgagggc gaggcgatct cctggcacct cctcgaggtg ctcaaggagc ggggcgcgct
    15481 caagggcatc gaggtgaagc gggtggtctt ccacgagatc acgaagcgcg ccgtgaccga
    15541 ggccatggcc catccgcgcg acctcgacca gagcctgatc gacgcctacc tggcgcgccg
    15601 cgccctcgac tacctcgtcg gcttcaccct ctcgccggtg ctctggcgca agctccaggg
    15661 ctcgaggtcc gccggccgcg tgcagtcggt agcactccgc ctcatctgcg agcgcgaggc
    15721 cgagatcgag gccttcaccg cccgcgagta ctggaccatc gaggcgctgc tcgagacccc
    15781 ggaccagacc cgcttcaagg ccaggctgac ccacctcgcc ggcaagaagc tcgacaagtt
    15841 cgcgctcggt gacaaggtcg ccgccgaggc ggccctggcc aaggtgcaag ccgccacgct
    15901 cgcgatcggg ccggtcgaga agaagcaggt gagccgcaac ccgtcacccc ccttctccac
    15961 ctcgaccctg cagcaggaag cggcgaggaa gctcggcttc tccgccgacc ggacgatgaa
    16021 gacggcccag cgcctgttcg agggcacgga gatcggcggc gagacggtgg gcctcatcac
    16081 ctacatgcgg accgattcgg tgcagctctc gcgggacgcc atcagcgccg cccgcaagcg
    16141 catcgcccag tccttcggcg acgcctatct ccccgagcag ccgcgcatct tcaagacgaa
    16201 gaccaagaac gcccaggaag cgcacgaggc gatccgcccg accgatgtcg gccgcgagcc
    16261 gaagacggtc gcgcgttttc tcgacaacga ccagagtcgg ctctacgagc tcatctggaa
    16321 gcggactatg gcgagccaga tggcggcggc caggctcgac cgcgtgaccg tcgacgtgct
    16381 cgacgagaag ggtgcggtgc agctccgcgc caccggccag accatcgcct tcgacggctt
    16441 cctcaagctc taccaggaag gccgggacga tcccttgggc gacgacgagg acggcgaggg
    16501 cgtgctcctg ccggcgctcg tcaccggcac gaaggtcgcg gagcgcggcg tcaccccgga
    16561 gcagcacttc accgagccgc caccgcgctt cagcgaggcg agcctcgtca agcgcctcga
    16621 ggagctcggc gtcggccgcc cctcgaccta tgccagcatc atctcggtgc tgcaggaccg
    16681 ggactatgtc cgcctcgagc agaagcgctt cgtgcccgag gaccggggcc ggctggtcac
    16741 cgcgttcctc accagcttct tcgaccgcta cgtccagccg accttcaccg cggccctcga
    16801 ggaccagctc gaccgcgtcg ccgccggcga gctcggctgg aaggacgtgc tgcgcgcctt
    16861 ctgggagccc ttcgtcggca gcatcggcga ggtcagcgag ctgcgcgtcg cccaggtcat
    16921 cgacgcgctc aacgaggcgc tctcggccaa gctcttcccg ccgcgcaccg atggcggcga
    16981 tccccgcatt tgcccgctct gcggcaacgg ccagctcagc ctgaagctcg gcaggtacgg
    17041 cgccttcatc ggctgctcca accatcccga atgccgctac acccgcaagc tcgacgacga
    17101 ccccaatggc gatccggcgg cccgcaacga cgagaagctg ctcggtgtcg atcccctgac
    17161 cgaggaggag gtctggctga agaacggccg cttcggcttc tacctccagt ccggcagcgg
    17221 cgacgcgctc cgccgcagct cgctgccgcc caacctgacc ccgacggaga tcgacctcca
    17281 gaccgccctc gcccttctgg ccctgccgcg catcgtgggc acgcaccccg aaaccggcgc
    17341 cgagatcacc gccgggatca accgctacgg ccctttcgtc cagcaccagg gcaagttcgt
    17401 ccggctcgag gcggacgacg acgtgctcag cctggggatg aaccgggccc tgacgctgat
    17461 cgccgagggc ggctcacgtg gccggcagcg cgccgccgcc gccccgctgc gcgagctcgg
    17521 tgcgcatccc gacacgggcg agaaggtggt gatcctggac ggccgcttcg gcccctacgt
    17581 caagcacggc aaaaccaatg ccagcctgcc caagggccgg ccggtggaag agctcaccat
    17641 ggacgaggcg gtggccctcc tcgcggcccg cgaggccaag ggtggcagca agggcaaggg
    17701 gcgcacgacc tcgcggaagg cgccagcgaa agccaagccg gccaaggcca ccgcggtgaa
    17761 gaagaagccc gctgccagga agcccgccgc caggaagaag tcggccgccg gcggcggctc
    17821 ctgagccatg cgccgggggc gcggccatgg cgaccggggg ccgcgcgatc gcagcaccgg
    17881 gcggcgcaac cgcttcgagc gcaccggcac gccgccgggc atggccgccg tcgaggtgac
    17941 cgccatcgac gacgcgggcg agctggtcgg cgagagcccg gccgtccccg gtgctgccat
    18001 ccgggtgctc gacgcggatg gcggcgaggc cccgctcgag gtcggcgacc gggcgctgat
    18061 gcgcctttcg accgatgccg agggtgcgat cgagggccgc ctctttcgca agctcggccg
    18121 ggtcgcccgc gatatcgtgg gtgtcgtcga ggcgagcggc gggacgctct tcctccggtc
    18181 gagccgccgg tccgccgacc gcgaccagcc gacccgcctg aagctcgagc cgggcgacct
    18241 cacgctggcg gatggcgaca tggtgcgcgc cgagctcgac cgcggccggc cgctcgccgc
    18301 accgcgtgcc cgcgccgtcg agcgcctcgg cagcagcacc gaccccgccg ccatcgcgct
    18361 ttcgatcgcc gccgagctcg agctgccgac cgtcttcggc cccgaggcgc tggccgtcgc
    18421 cgagcgcgcc acccggccgg cgctcggcga gcgcacggac ctgcgcgatc tttcgctcgt
    18481 caccatcgac ggcgccgacg cgcgcgactt cgacgacgcg gtctgggccg accccgagcc
    18541 gctcgccgat ggcgggtttc gcgccgtggt cgccatcgcc gacgtcgccc actatgtccg
    18601 gccgggcgat cccctcgacc gcgaggcgct ggagcgcggc aactcggtct atttccccga
    18661 ccgggtgatc ccgatgctgc cggaagccct ctccaacggc ctctgctcgc tcaagcccga
    18721 cgaggaccgc gccagcatcg ccgtcgagct cttcttcgat tcgggcggcg tcctgaagcg
    18781 ctggaatttc acgcgcgccc tgatcaggag ccgtgccagg ctcacctacg agcaggtcca
    18841 ggacgccgcc gacggccggc ccgacgccct gaccgccccg ctcctcgaat ccgtcatccg
    18901 gcccctctac gacgtttacg agaagctcgc cgccgcccgc cgcaagcgcg gcacgatcga
    18961 gctcgagctg cccgagcggc gcgtcctcct cgacggcgag ggcaaggtca ccgacatcgt
    19021 ccagcgccag cgcctcgcca gccacatgct ggtcgaggag ctgatgatcg ccgccaacgt
    19081 cgcggccgcc cgcgccctcg ccgaccggaa gctgccctgc ctctttcgcg tccacgacaa
    19141 gcccgacccc gtccggctcg aggccctggc gcagtatctg gagcgcctct cgatcggctg
    19201 gagccggacc gccaagaagc ccggcgactt cacggccatg ctcgagcgga tcgtcgagcc
    19261 ctccctgcgc gagcaggtct cgggcttcgt cctgcgcgcc caggcccagg ccctctacca
    19321 gcccgacaat ctcggccatt tcggcctcaa tctccgccac tacgcgcact tcacctcgcc
    19381 gatccgccgc tacagcgacc tcgtcgtgca ccgcctcttg atccaccacg agaagctcgc
    19441 cggcaccggg gacggccgac cttatgacct cgagcagctc gaccgcttcg gccggctggt
    19501 ctcggccacc gagcgcaagg cggtccaggc cgagcgccag gcgctcgccc gctacaccgc
    19561 cctctacatg gccgacaagg agggggcgca gttcaccggc cgggtcatcg gcgtgcagcg
    19621 cttcggcctc ttcgtcgtgc tcgacggcac cggggccgag ggtctcgtcc cggtcagcac
    19681 gctcggcgac gacgccttcc atttcgacga gcagcaccag gccctggtcg gcgtccgcta
    19741 cggcgacacc ttcggcctcg gcgatgccgt cacggtgacc ctcgccgatg tcgacctggt
    19801 gctcggccag ctcgcattcc gcatcgacag ccacgagcgc gcccacgccg ccgagctcgc
    19861 ccgcaccgcc tgggccaagg ccggcccgat gcgccggggc cgcggttcgc cgaaccggcc
    19921 gcaccgtgcc tccggacgcc gcggcaagcg ctgaccccgc ctcgagccgg aggctcgagc
    19981 tcgcggcgct catggtgagc gctgccgtgg tcggcacggg cgtggcgttg agctttcccc
    20041 tcgtggccct ggtgctcgag gccgaggggg cgacagccac ggcgatcggc ctgaacgccg
    20101 cagcgcatgg cctcggcgtg ctgctcgccg cccctttctt cggccgcacc gtgcgcaagc
    20161 tgggtgctgc ccgcggcatg cgcgtcggcc tgacgctggc cgccatcctg ctcctcggtt
    20221 ttccgctcgc cacggacctc ggctactggt tcatcctccg tctcggcttc ggcgccgcca
    20281 ccgccctcgt cttcgtcatc agcgaggccg gcatcaacgc gctcgccgag gacggccggc
    20341 gcggccgggt cctcgccatg tacggcacga tgttcggcgt cggctatgcc ctcggcccct
    20401 tgatcctggg cctcctcggc agcgatggcg ccttgccttt cctggtcggt gctgccgtgc
    20461 tcgccgccgg catcctgccc accttcgccg tgccctcgat cgacggcgcc ctcgacgggc
    20521 gacccggcgc ctacgccgcg cggcgcctca tgcgcctcgg ccgggccatc ccgctcgtcc
    20581 tcctcgtcgc cctggtcgtc ggctggaccg agacgagcca gttctcgctg ctcccggtct
    20641 gggcgatccg gcacggcctc gacgagacga cggtggcctg gctgctcggc ctctgggtcg
    20701 ccggcgacat cctcttccag tacccgatcg gctggctcgc cgaccggctg ccacgccgcc
    20761 ggctgctgct cgccgcggct ctcggcgcga ccctcctcgc cctgacgctg accacgcctc
    20821 tggtcgccgc cacaccctgg ctgctgattc ccgccctcat tgcccacggc gggctgatcg
    20881 gcggcagcta cgtcctggcg ctcaccctca tgggcgagcg cttccgcggt gctgctctgg
    20941 tcggcgccaa cgccgccttc gtcctcgcct tcgagatcgg tgcgatgatc ggcccacccc
    21001 tcaccggcct cgccttcggc gccatcggca ccgccggcct gccgctggct ctcgccctcg
    21061 tcacggcaac gatcgtcgcg gccgggctcc acgcacgccg cgccagcgcc ttgtcgaggg
    21121 cctagatgtc ccgctcggcg cccggagcag cggcagatga ctggttcgtg agcccggaca
    21181 cgctttaccg cgcggtttga cggattatct ttctggtgcg gtcacgcgac gacatcgcgc
    21241 gttgcaacgt cacattccca cgcgcgtggt tcggcagcga cctccgctcg ccacgcagac
    21301 gaggagagac caggatgacc agggttcttc gaacccgccg cgccctgctt ctggccggtg
    21361 ccgccagcgc cggagccgcc tttcttggtg ctggcgcctc gggcctgctt gcgcccgtgc
    21421 gcgcgcaggg cctggcggcc acgccgacga tgcggggcgg cgccaacaac tatcggccgg
    21481 gcgcgcccct cgtggagcgc atcggccatg gcggcttctg gatgaccggc acggtgcgac
    21541 gagcgggcga cggcgcgccg ctgccgggcg tgcgcatcca gatctgggcg cacacgaccg
    21601 agggccacga gcgcgacccg cacagccacg gcgccacgct gaccgatgcc gacggcgtgt
    21661 tccggatgga catgccgcag atcgttcccg ccttcggcca gccgcacggt cacctcgcct
    21721 acgaccccga gttcgacggc cgcgagcatg acggcagggg cttcgagacc gtgttcctcc
    21781 gccccgtgat ggcgagcgcc agcgagacga gcctgagcgc cgacttcgtg ctgcagcccg
    21841 cctgagcatt tggtcccggt ccgcgccatc ctgatctggg cggccctcgc cgccgccctc
    21901 gtcgtcccca tcgccgtcgc ggcgacgagc cccctcctcg cctggcggca gccgatctac
    21961 atcgccgccg gcttcgccgg catcgtcgcc atggcgctcc tcctcctcca gcccctcctg
    22021 gccggcgggt acctccccgg cctgccgctc cgccgcagcc gccgcctgca cgcctggatc
    22081 ggcgccggcc tcgtggccgc ggtggtcgtc cacgtcgcgg gcctctggct gaccagcccg
    22141 ccggacgtca tcgacgccct cctcttccgc tcgcccaccg ccttctccgc ctggggcgtg
    22201 atcgccatgt gggccgtctt cgccaacgcc ctcctcaccg catcccgccg tcggctgcgc
    22261 ctgaggtcga ctgtctggcg cttctggcac accgcgctcg cagccgtcat cgtcccgacc
    22321 agcgtggtcc atgcgctgct gatcgagggc actatgggca cgctgtcgaa ggcggcgatc
    22381 tgcgcgctgg tcctggcggc agcggtgaag gtcatcgtcg atcggcggtc gtggaccctt
    22441 ctgtcgcggc agaaggtcta ggccggagcc catt
//
LOCUS       DAOTFI010000044        22372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2742_length_22372_cov_8.982928, whole genome shotgun sequence.
ACCESSION   DAOTFI010000044 DAOTFI010000000
VERSION     DAOTFI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22372
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_22372_cov_8.982928"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..690
                     /locus_tag="P5558_06275"
     CDS             259..690
                     /locus_tag="P5558_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23948.1"
                     /translation="MFQIKAAISKGPSGDTKMVVDESRDVWLQRVGEVLFTRGRVFRL
                     VWKGNVIPFETTTEPRRDERGKDYISVRFRTFGSSVHAERHGVGPYHFSGEEERRQAM
                     ILAVEALRTFGGNYSGPDNPEGFHRIEHDGRIYTKRDFGMA"
     gene            1071..2021
                     /locus_tag="P5558_06280"
     CDS             1071..2021
                     /locus_tag="P5558_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRY23949.1"
                     /translation="MLGRLLAVLAYLSLSTDLAQVQPTRAGSDGSSGNPFAYDDANES
                     VAFESDHDVPITPLESTDGRRRLRVVIITEDDPLYVIEFFRVFFDEFPCGEIEVCGMT
                     IDRAFHEPLQHTLLRMMRFYGWFGTFFQGVRFVKAKVSRSTIASLADEHGVTVLETES
                     VNDRAYLDRVRSLRPDLIASVAGPEIFSSDLLELAPLGCINVHSGRLPTYRGMLPCFW
                     QMVRGEPEVTITVHRMVEKLDAGDILATQAFPIRASDSLDRVIRGTKRAGARLFIEVL
                     RDLRTGVAQGTPLDWSKAGYFRFPNSTDVRKFRRRGHKLL"
     gene            2278..3060
                     /locus_tag="P5558_06285"
     CDS             2278..3060
                     /locus_tag="P5558_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRY23950.1"
                     /translation="MLVDDSPYGVPPVARAFTLLRYVAAGNRCRNTSQAAKATGINRT
                     TLIRLLATLEEEGMIEAIPDDGGYRLGTGLITLAAEALHGRDIAQVSRPHLVELVREL
                     NLSAHLGILEGREIVYLARVTPNSHLASNVREGTRLPAHATSIGRILLADLAPEELRK
                     RYAGVRLDAYSDKTRTSLADLEKQLKEDKALGVAWSMANFEPEIGSGAALVRDHRGQA
                     AGAINVTGHASAFSPGNPRLPEIEAALKKAATGISEALGFRG"
     gene            complement(3165..3650)
                     /gene="bfr"
                     /locus_tag="P5558_06290"
     CDS             complement(3165..3650)
                     /gene="bfr"
                     /locus_tag="P5558_06290"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRY23951.1"
                     /translation="MKGNPKVIELLNDALRHELTAVSQFWLHYRLLQNWGYGEMAQKW
                     RDESIEEMEHADQLIARIVFLEGFPNLQKLDPLMIGDTIREVLDADLKAEYRARALYR
                     EARDVAEEAEDYVSKGLFETLMDDEEGHIDFLETQIDLFDRIGAEEYGLLNATPADEA
                     K"
     gene            complement(3647..3889)
                     /locus_tag="P5558_06295"
     CDS             complement(3647..3889)
                     /locus_tag="P5558_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23952.1"
                     /translation="MIICSCAVIRQEELREVTRKLYRSNPGAPITPNRVYRALGKSPT
                     CMDCAPLLTLRIYATAQEVIVAEGLLRGDAHPRRIK"
     gene            complement(3919..4704)
                     /locus_tag="P5558_06300"
     CDS             complement(3919..4704)
                     /locus_tag="P5558_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY23953.1"
                     /translation="MASQAVIVDGSVYLVDCGYGTILRMVEAGLRPADVKAIFVTHHH
                     SDHNADYPNVVHLAWIQGIDGQIVSVGPPPFQSIHTAALAFHHEDIRIRIAGTGRQPI
                     EGFFEVREVAEPGLVYQDDKVRVLAGIADHPPFSTALGFRFETPERTIVFSGDTAASE
                     SIAVLAEGADVLVHEAMFPAGIDAMLAKRPYVPPKLRQFLMEGHTSAEDAGRIAAKAQ
                     VGTLVLSHLLPGDQPEVTDEIWRAEAARHFDGPIIVGRDLMLV"
     gene            complement(4940..6073)
                     /locus_tag="P5558_06305"
     CDS             complement(4940..6073)
                     /locus_tag="P5558_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRY23954.1"
                     /translation="MGLEIGICGGGVGGLTAAIALAKDGHAVTVHEQARAFARIGADV
                     NLTPNAVHALDRLGVGETLRETAARPRFRISRTWDSGEETSRLPMGDEAERKYGAPQL
                     TIHRGDLLRALEARVPQSSIRLGHRVTAVSDGTVTFADGSSERFDVVIGADGIHSAVR
                     ASLFGEDHPRFTGLVSYRAVVPRDAVAAENLDSFTKWWGPRPDVQVVVFPLTRGEEIF
                     IFATTPQDDWREESWTLPGDVAELRAVYADFHPEVGALLGAVASVTKSALYVRDPMPR
                     WSSGHATLLGDAAHPMVPFMAQGACMAIEDAVVLSRALAGATRADVPDALRRYEDARR
                     DRTARVQLGSLANDWMKGQGNADWVYGYDAWAAPLGAPAETAG"
     gene            complement(6088..6687)
                     /locus_tag="P5558_06310"
     CDS             complement(6088..6687)
                     /locus_tag="P5558_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRY23955.1"
                     /translation="MRAAEILERLSVAWKHGEHVDLAGVTCEGRLDLSGREIPGVDFS
                     GATFPEGIDATGTRFLGLAWFRGVAFEGPSPFARAQFLTDARFEDASFDAPADFSKVE
                     FRGIARFDHARFAQGASFAGTVSYGNVSLAHADFGAPADFRGAEYLGGIWCDSTAFAE
                     GTDFSDIQVHGRLWIRRARLGTAPLAADRFTLSFGYTYA"
     gene            complement(6687..7154)
                     /locus_tag="P5558_06315"
     CDS             complement(6687..7154)
                     /locus_tag="P5558_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRY23956.1"
                     /translation="MNITVLYGTETGNAEMLAEDVTAHLEGDHEVTCTNLSDFAPSDF
                     APEQFYLVICSTYGDGELPSSAKPFAEAMETAQPDLAGIHFAIFGLGDTEYDETHNFG
                     SQIIADMLKGRGAAQVGDRVIHDASGSDMADDLAMEWADAVVELARTRVGEAA"
     gene            complement(7158..8378)
                     /locus_tag="P5558_06320"
     CDS             complement(7158..8378)
                     /locus_tag="P5558_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY23957.1"
                     /translation="MQKSVAIVGSGPSGCYLAQALLKAKPDLKLDLIDRLPVPYGLVR
                     YGVAADHQGTKAIIRQFERLFERQGAGFIGNLDIGEDVSLDELRAAYDAVVLAAGLAT
                     DRRLGIPGEDLAGIAGAGTVTRALNEHPDADPLPVLGRHVVVMGNGNVAIDIVRLIAK
                     SADELAGTDLGPVPTAWLTAGHIETIDIVGRSAASHAKFDPVMVKELHHLAHATIKVE
                     GAGTAGEPAGQKKLDALAAIDGIAHGPRRLTFRFGLTPTAFEGAEGRVTAVRFHDAAG
                     AETVLPCDTFISAIGFDTGANLSRGALLEHARDIDAGVLDDGLYATGWFRRGPRGTIP
                     ENRAEAQALAARVVADLDAATGRPGLPATGRVDYAGWKRIDAAELAAATADRCRRKIA
                     DRAAMLALALNNEG"
     gene            complement(8378..9859)
                     /locus_tag="P5558_06325"
     CDS             complement(8378..9859)
                     /locus_tag="P5558_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRY23958.1"
                     /translation="MTDLPDGKLYVAGEWTTGSGAEITSIFPADGSVNRVLKGASEAD
                     GLAAIERAKGAQADPAWRNLRPHERARILTRIADGIEANVDRISRIQTMDTGKTLKET
                     RALAASAAGTFRYFAAALETADEALTTQRGDALTMSVHEPLGLVAAITPWNSPIASDA
                     QKVAPALAAGNAVLLKPASWSPLVSLELARIVEEAGLPKGLFSVLPGAGREIGNLLVE
                     HPDIAKVSFTGGTSTGRALARKAAEKLMPVSLELGGKSPTIVFADADIDQAIAGVLYG
                     IFSSSGQSCIAGARLFVERPIYDSFVERLTAATERLRVGHPFDPATQVAPLVHFDHRA
                     SVAKMVDAARAEGAEVLAGGRAPAGETYDKGAFYLPTILTGVDNSATICREEVFGPVL
                     VVLPFEDEADVIAQGNDSDYGLASGIWTRDFPRAWRIGRALRTGTVWINTYKQFSIST
                     PFGGEKESGVGREKGRDGIRAYMAQKSIYTDLTGKPLAWAGAA"
     gene            complement(9888..10706)
                     /locus_tag="P5558_06330"
     CDS             complement(9888..10706)
                     /locus_tag="P5558_06330"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HRY23959.1"
                     /translation="MRLGLIGFGNIGTALLGLIAEGAPLDHLIVLVRPGAADAARSRL
                     DGELKGAARSHALVSDAAGLLADRPDLVVECAGHSAAEAHAPAVLRAGTDVVLVSTGA
                     LADPALEAALKAAAAEGGARLILPAGAVGAIDLLSALGAGGGLEVRYRGIKPPAAWAG
                     TPAAENLDLASLAEPAIFFTGNAREAATAYPKNANVAATLALAGAGFEATRVELMADP
                     SAPDNVHEYSVVSPIANYTIRIENKPSSGNAKTSATTIYSVLREIRNRLGPVAI"
     gene            complement(10711..12414)
                     /locus_tag="P5558_06335"
     CDS             complement(10711..12414)
                     /locus_tag="P5558_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY23960.1"
                     /translation="MLLATIDDTETPGDTAAGIETVGDFIARFLAEIGVTTIFGVISI
                     HNMPILDAVARQGKIRFVPARGEAGAMNMADAYARVTGGLGVCMTSTGTAAGNAAGAQ
                     AEALTAGSPVLHITTQVDREFADRDRAAIHDVPRQPEMLRGVSKAVFRMWDANGAIGA
                     LTAAVSAALSAPTGPVSLEIPVDVQRSTAHGTTRIHAPRATRPIASDAVLDEIAELVK
                     KARRPLLWLGGGARGAGAPATELLKRGFGVVTSTNGRATVSEEEPGNLGAFNMTPEAA
                     ELYASSDLMIVVGSRLRGNETRNNQMPLPRPLIQIDADAGQGGRNYPVEIFAHGDATD
                     TLRRLLNRLPAKLDTDPNLPFDIARTRVQSEGRMRNVLGPYAGVAEALTERVAAGRHP
                     WVRDVTISNSTFGNRYVRIANPRHGVHALGGGIGQGVAMGVGAALGSSGPKAITLLGD
                     GGTMLGLAEMITAVDENAPLVYVLMNDQAYGVIQNIQDAQYDSRRHFSKVAVPEFPTF
                     CRAIGMPHTIVRSVEDFPAALDAALAADGPQVVEVDMVAIGPFAESFSGPPAGAAGNK
                     V"
     gene            complement(12426..13214)
                     /locus_tag="P5558_06340"
     CDS             complement(12426..13214)
                     /locus_tag="P5558_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23961.1"
                     /translation="MDYGYQGKVAVVTGGSSGIGLATVRLLLEHGAQVAFCARNEGRL
                     HDVACVLTRDHGQERVLARALSVLERASVDAFAAEVRDRFGRCDLLVNNAGQGRVSSF
                     AETEDDDWRTEYELKLFSQIHPTRAFMPLLRESRGAIVAVNSLLAYQPEPHMVCTSSA
                     RAGVQNLLKSLSVELAPEIRVNSVLLGLVESGQWTRRFAEREDQSQSRAEWYGNLAAR
                     KGIPLGRLGDPSEAAAAIAFLGSRAASYITGAQLDVSGGLARHI"
     gene            complement(13214..14128)
                     /locus_tag="P5558_06345"
     CDS             complement(13214..14128)
                     /locus_tag="P5558_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY23962.1"
                     /translation="MTEPVRVGSLRGIMMRGPGVSATLPFYEDMWGLSLSHKEDGVAL
                     LRGTGTEPFLYGLKDGPVFGIEYVHFAMPDRASMDALHAQIIARGHTPISAPGAFDDW
                     AGGYGFEMLDPDNRRLRFRTEARVLDDQKDWAKPKKVSHVVLNTPDMDGTQKFYEEVL
                     GFRASDYSADQMVFLRCNSDHHSIALVRGNYASVNHVAFEMPTLDEFMRGIGRMKQKG
                     HVPSWGPGRHGPGNNPFAYFVSPSGFVIEFTSELQQIDEATHEAKVWSRTDPEAMDRW
                     MTAGPPTPAQRAVMQGRPDPGFPELRTR"
     gene            complement(14156..14962)
                     /locus_tag="P5558_06350"
     CDS             complement(14156..14962)
                     /locus_tag="P5558_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY23963.1"
                     /translation="MFTAQTSAGIDYRERPGSGPSLVLLHGIGSNADSFAELLPLLPP
                     DRQVVAWNAPGYGASAPLGPDWPVAADYADALEAFLDRLGLDRVTLVGHSLGTLMGAA
                     FAVARPARVERLLLASPALGHGVAPGGALSPAAQARIDDLARLGPEEFARVRAARLVY
                     RPEENRKLVAAVEAGMSKVRSPGYDQASRMLASGRLLEDAARLAVPTDVIVGEEDIVT
                     PPEGARALHAAIPALVRGRLTEVPAAGHALYTQAPLAFARAVAPQATPVA"
     gene            complement(14974..15711)
                     /locus_tag="P5558_06355"
     CDS             complement(14974..15711)
                     /locus_tag="P5558_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY23964.1"
                     /translation="MLAGRSILVTGAGRGLGAEIARGLAGHGARLVLADIAEDEGRAA
                     AEALGATFAPVDLADPDSIAALAATVGEATGGRLHGLVNNGAIATGIGGIPFEEIEIE
                     SWDRVQRVNVRGTWLMTRAAAPLLRASGSGRVVNIASDTAIWGAPRLLSYTASKGAVI
                     SMTRSLARELGPDRVGVTAVAPGILTTSSTDYVPEARHQLYSDGRAVPGPQAPGEIVD
                     TIAFLLSEGALTLTGQLLPVNNGFVFA"
     gene            complement(15711..16754)
                     /locus_tag="P5558_06360"
     CDS             complement(15711..16754)
                     /locus_tag="P5558_06360"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRY23965.1"
                     /translation="MTAIDKKTLIEERVNTGLLGQWYPVAKSVEVKGTRPHGVKALGQ
                     KLVLWRDASGKINCIEDFCPHRGAPLSYGEVHEGNIGCRYHGVIVNGEGVVVRVPAMP
                     ECALEGRKALKSFATTESNDAVFVYFPSVEQPEPPALVLPKELVSDAWTGFLCTSVWE
                     CNYRYALDNLADPMHGCYLHSESFTLAYGSKQDLMKLDKTENGFHISRVGQVGENFDW
                     SEFEIHAGDMFCFLDIPYPPAAGPGGFMRIIGFTLPVDEHTCKVFFWRMREVSGVARD
                     SWRFLYRAVLEPNHWAVLEQDRVMLEGMPDDARKREMLYQHDLGVSRIRQILTRAAKS
                     QVEAELAQAQAAE"
     gene            complement(16811..17365)
                     /locus_tag="P5558_06365"
     CDS             complement(16811..17365)
                     /locus_tag="P5558_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY23966.1"
                     /translation="MSAVPKKFPSIEPEDVMIPEGKSLGDWIESRTARYATRTLDWDA
                     LKFQADYDPIYRRAQMRYIGTGATGVTDDENVIPAENFTFSTMVLPAGCEGPLHIHRD
                     AEEVFFILKGHKVRFFIEHKGELVETVLSERDMVSVPPGLYRGLRNEGIEEALMCVLI
                     GNPKPVTPTYPPDHPVSQIKRDKK"
     gene            complement(17413..18234)
                     /locus_tag="P5558_06370"
     CDS             complement(17413..18234)
                     /locus_tag="P5558_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY23967.1"
                     /translation="MSSATATAPAVNAAATTRPRESRAALYASLGLLALLLVAWQILP
                     TALAVPKYIIPTLSDCLVEFGRMWTSESLLANTASTALYTILGFAIGSALGAALGYLL
                     GMSEFWEKVLSPYILALQIAPKVAFAPLFIMWFGYNAMPKLLVTVLIVFFPVLVNVLQ
                     AMRTVDRDLVNLARAYNLSRFQIFLKVEMPSTLPNLMAGLRIASTLAVIGVTVGELVG
                     GNTGLGFLISYGGGQANAAMVFNAIILLTVIGFLLYSALVRAETRLLHYLPKAQF"
     gene            complement(18231..19202)
                     /locus_tag="P5558_06375"
     CDS             complement(18231..19202)
                     /locus_tag="P5558_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY23968.1"
                     /translation="MKRLFLTTVAAAAISFAAAADAAEEVTYLFPAPDFLPAFAPFQL
                     AKAKGYFDEAGLDVTFQVGKGGADVATQVAAGNADMGGGIGDTPIIVRANGLDIRGVA
                     LLGGRGLTQLAWRKDSGITGPADFKGKNVGVLSFQDTTYYNLLGVLASAGLDKSEANI
                     QGVGPGGIIQLMIAGNLDAMSGVPEWIAAVEGGGVEVEQMPVDEVFPAMAQAIIASDA
                     TIAERPEVVKGFVNAVLHAVRDIEADPAQAAQDFVAAVPQHAGKEAEVQAILEAYAAK
                     VYPQAEGQPLGTFDPERIKAVQDFYMDAGVVQTAIPVEDLYTNEFVQ"
     gene            complement(19234..20004)
                     /locus_tag="P5558_06380"
     CDS             complement(19234..20004)
                     /locus_tag="P5558_06380"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY23969.1"
                     /translation="MALEFRGVTKQFTTNSGAVLTAIRDVTFKVNEGEFVSVVGPSGC
                     GKSTLLSMTAGLYQPTTGEVLVSDQPVTGPNDHVGFMLQKDLLLPWRNIVKNIEFGLE
                     ARGAAPGPRRERAMAELKRCHLAGFENHYPYQLSGGMRQRAALARTLAIDPEIILLDE
                     PFSALDAQTKLLLQDSFAATIAESGKTTLLITHDLSEAVLMSDRILVLSERPGTVLTE
                     IKVELPHRAHPLKRRVMPEVGEYAGQIFRHLKLEEKAI"
     gene            complement(19994..20296)
                     /locus_tag="P5558_06385"
     CDS             complement(19994..20296)
                     /locus_tag="P5558_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23970.1"
                     /translation="MDAFKDRLSQRARDTSRPALAHQCRGREITPAEAALAEALMAIY
                     GEGTHDYAAVAEALAAKGVTAPMSGRTDWTEALLTAELAGINGELDAAYAEHGYGA"
     gene            complement(20818..21885)
                     /locus_tag="P5558_06390"
     CDS             complement(20818..21885)
                     /locus_tag="P5558_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRY23971.1"
                     /translation="MLRTAPVRIGATLLLAGGGVVVFGALDLPLPWLLGPMCAGLLAS
                     LGGMPVTAPPGIGDAMRTVLGVAVGASITPELITRLPDMALTAAMVPLMVVLIGLVGV
                     PYFRRLCGFDAPTAYYSAMPGGFQDMVLLGEEAGADPRTLSLVHATRVLAIVAAMPLL
                     VSSIWGRTLDAPPGLPAAGFPARELAIMVFCAAAGWWLARRARLFGAAILGPMILAAA
                     ASLAGLIHARPPAEAILAAQFFIGLGIGAKYRGITLAELRTVVLAALGFCAILTGISA
                     LFAEAAVLLGLAEPVEALLSFAPGGQGEMVVLAIVSGADMAFVVTHHVVRLVVVILGA
                     PVFGRGVPRLPCPGRRAPPRP"
     gene            complement(21897..>22372)
                     /locus_tag="P5558_06395"
     CDS             complement(21897..>22372)
                     /locus_tag="P5558_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY23972.1"
                     /translation="DVVWGLIAALFIANFMLLAMNIPMVGLFARVLMIPPKFLMPIVA
                     MISFVGIYGISGSTFDLLVMVIFGVLGWVLRKLDVPLVPVILGILLGNLMEANLRRAM
                     TISDGDWTVLFTSPLAIILWTIAVVGFVLPILVGKRIKRRMHMAEEEPGGATSVD"
BASE COUNT         3470 a         7084 c         8326 g         3492 t
ORIGIN      
        1 gatcattcca gtcaggagca ccgacggcga agccctcacc ctgccgcgca gcggcctcgt
       61 tcaatcccct cacgattcac aacgctcggg aaccgcttta actcattgct aaccggcttc
      121 tttgagtcct gagtgtggtg tgctctgtgg acgttggccg taccgcagag ggaagcgata
      181 tgggatgccg gggcatcata ggcccggatg gaagtattga acagaggagg ctggaagcga
      241 tctgagggca gtgcgctgat gttccaaata aaagctgcga tctcaaaggg accaagcggc
      301 gacaccaaga tggttgtcga tgagtctcgt gacgtctggc tgcagagagt tggcgaagta
      361 cttttcacta gagggcgcgt tttccgacta gtgtggaagg gaaacgtcat ccccttcgaa
      421 acgacaacgg aaccccgccg agatgagcgt ggcaaggact acatttcggt gcggttccgc
      481 acgttcggca gcagtgtcca tgccgagcgc catggagtcg gtccgtacca tttcagcgga
      541 gaggaggagc gacgacaggc aatgatcctg gcggtggaag cccttcgcac atttggaggc
      601 aattacagcg gccccgacaa ccctgaggga tttcaccgca tcgagcatga cgggcgtatt
      661 tacaccaagc gcgattttgg aatggcctag gcagttcgtg cgccaatccc gatctgcagt
      721 gtacaatacg caacgcccgc actcgagtct cggccacaag ccgccggcgc cggaagcact
      781 gctctggccg ggcgagccgt caaacttggc cgcaaagccc ataatgcatt agatttgaac
      841 cgggccacca aatggggcag accagtagcg ggacaccggg ttcgctccaa ttcatctggg
      901 gttgccttcc gcgtgtggcc gtagcgacaa gtcaacccat tgcgagcaaa ttctctgtgg
      961 cacgccaatc tgaaatccgg cctcttccat cttgatgctg aacgatgaat aacggaatgt
     1021 gctaactgca ttcggttaac ttgacccagg cgagcaacag gaggtccgct atgctgggaa
     1081 gactattggc ggtgcttgcc tatttgtcac tgtcaacgga tcttgcccag gttcagccta
     1141 ctcgggccgg cagcgacggc tcgtcaggca atccgttcgc ctacgacgac gccaacgaat
     1201 cggtcgcctt cgaaagcgac catgacgttc cgatcactcc gcttgaatct accgacggaa
     1261 ggcgcaggct ccgcgtcgtc atcatcaccg aagatgaccc gctctacgtg atcgagttct
     1321 ttagagtctt cttcgacgaa ttcccttgcg gggagatcga ggtatgcgga atgacgatcg
     1381 accgggcatt ccacgaacca ctacagcaca cattgcttcg catgatgcga ttctacggct
     1441 ggttcgggac gtttttccag ggggtacgtt tcgtcaaggc gaaggtgagc cgctccacaa
     1501 tcgcgtccct ggcggacgag cacggcgtga cggtgcttga gacggaatcg gtcaacgaca
     1561 gggcctatct tgatcgcgtt cgcagccttc gtccggacct gatcgcatcc gtcgcaggcc
     1621 cggagatctt ttcgagcgat ctcctggaac tggcgccctt aggttgcata aacgtccact
     1681 ccggccgctt gccgacgtat cgcggcatgc ttccgtgctt ctggcagatg gttcgtggcg
     1741 aacctgaggt gaccatcacc gtacaccgca tggtcgagaa gctcgatgcc ggggacattc
     1801 tggcgacgca ggcgtttcca attcgggcat cggactcgct tgatcgggtg atccgaggaa
     1861 cgaagcgtgc cggcgctcgg ctcttcatcg aggttcttcg cgacctccgg accggcgtcg
     1921 ctcagggaac cccgctcgac tggtcgaagg ctggctactt ccgatttccg aattcgacag
     1981 atgtccgcaa gttccggcgc cgcggtcaca aactgctgtg aggggacacc ggagtacttt
     2041 tccggaccat tcggcgtatc tcgcccgggt actgctccag agcgtttccg gctcgtggca
     2101 acgacctatg accggaaaag acgcatggac ccgggaaatc cggttcttgc gttgagcgga
     2161 tagccaatgc gtgcaatgcg agatcctcaa aggagttgca ttgcacactg gctttgacca
     2221 atagtgacct gttcatatga gtcatcaggc gttcacaaaa tggcagaagc atcagccttg
     2281 ctggtcgacg actcccccta cggggtgccg ccagtggcgc gcgcgttcac gctgctgcga
     2341 tatgtcgccg ccgggaacag gtgccgcaat accagccagg cagccaaggc cacggggata
     2401 aaccgcacga cgctcattcg gcttctggcg acgctggagg aagaggggat gatcgaggcg
     2461 atacccgacg acggggggta tcgtctgggg acggggctga tcacgctcgc ggccgaggcg
     2521 ctgcatgggc gggacatcgc gcaggtgtcg cggccgcatc tcgtggagct ggtgcgggaa
     2581 ctcaatctgt cggcgcatct cggcattctc gaagggcggg agatcgtcta tctggcgcgg
     2641 gtgaccccaa attcgcatct ggcgagcaac gtgcgggagg ggacgcggtt gccggcgcat
     2701 gcgacctcga tcgggcggat cctgctcgcg gatctggcgc cggaggagct ccggaagcgc
     2761 tatgccggcg tgcggctcga cgcctacagc gacaagacgc ggacctcgct cgcggatctc
     2821 gagaagcagc tcaaggagga caaggcgctc ggggtcgcct ggagcatggc gaatttcgag
     2881 cccgagatcg gctcgggggc ggccctggtg cgggaccacc gcgggcaggc cgccggggcg
     2941 atcaacgtga cggggcatgc cagtgccttc tcgcccggaa acccgcggct tcccgagatc
     3001 gaggcggcgc tcaagaaggc ggcgacgggg atctccgaag cactcggatt ccgaggctga
     3061 accctcgcct gagcgcgcgc gcacaagcgg tcaagtctct gatatagctg aagaacgcct
     3121 tcggcgttaa ccgttttgca acgggtgtgc gcgagggagc gggatcactt cgcctcgtcg
     3181 gcgggggtgg cgttcaggag gccgtattcc tccgcgccga tccggtcgaa gagatcgatc
     3241 tgggtctcga ggaagtcgat gtggccctcc tcgtcgtcca tcagggtctc gaagagaccc
     3301 ttgctgacgt agtcctcggc ttcctcggcg acgtcgcggg cctcgcgata gagcgcacga
     3361 gcgcggtatt cggccttgag gtcggcatcg aggacctccc ggatcgtgtc gccgatcatc
     3421 agcgggtcga gcttctgaag gttggggaag ccctcgagga acacgatgcg ggcgatgagc
     3481 tggtcggcgt gctccatctc ctcaatgctc tcgtctctcc atttctgcgc catctcgccg
     3541 taaccccagt tctggagcaa tcggtagtgc agccagaact ggctgaccgc ggtgagctcg
     3601 tggcgcagcg catcgttgag gagctcgatg accttgggat tgcccttcac ttgatcctcc
     3661 gcggatgtgc atcgccgcgc agcagcccct cggcgacgat gacctcctgc gcggtggcat
     3721 agattcgcag ggttagaagc ggagcgcaat ccatgcaggt cggagatttg ccgagcgcgc
     3781 ggtagacccg gttgggggtg atcggcgcgc ccggattgct ccgatacaat ttgcgggtta
     3841 cctcgcgcag ctcttcctgg cggatgacgg cgcaggagca aatgatcatc gccgcttctc
     3901 tccttctccg cgtatctttc aaaccagcat gaggtcgcga ccgacgatga tcggtccgtc
     3961 gaaatgccgc gcggcctcgg ccctccagat ctcgtccgta acctccggct ggtcgccagg
     4021 aagcagatgc gacagcacga gcgtgccgac ctgcgccttg gcggcgatgc gtccggcgtc
     4081 ctcggccgag gtgtggcctt ccattaggaa ctgccggagc ttgggcggca cataggggcg
     4141 cttcgccagc atggcgtcga tcccggccgg aaacatcgct tcgtggacca gcacgtccgc
     4201 cccttcggcc agtaccgcga tcgactcgct cgcggcggtg tcgccggaga agacgatggt
     4261 gcgctcgggg gtctcgaaac ggaagccgag cgcggtcgag aaaggcggat ggtcggcgat
     4321 gccggcgagg acccggacct tgtcatcctg atagaccagg cccggctcgg cgacttcgcg
     4381 cacctcgaag aagccttcga tcggctggcg gcccgtcccg gcgatccgga tccggatgtc
     4441 ctcgtgatga aaggcgagtg cggcggtgtg gatcgactgg aagggcggtg gaccgacgct
     4501 gacgatctgg ccgtcgatgc cctgaatcca ggcgaggtgg accacgttcg gatagtcggc
     4561 gttgtggtcc gaatggtggt gggtgacgaa gatggctttc acatccgccg gtcgcagccc
     4621 ggcctcgacc atgcgaagga tggtgccgta gccgcagtcc acgagataga ccgagccgtc
     4681 gacgatgacc gcctggctcg ccatcatccg ccgtggatcg aggaccggcc cggccatcgt
     4741 gccgagcaga aggaggtggg ttccggtctc gggcaccaca agggcttcgg tgccgcacgc
     4801 cggcgcctcc tctgcccggg cagccccacc aaggagaagg gccccgccgg tcaaccccag
     4861 gccggccgcc agcaactcgc ggcgatccat cggcatcttc cctccattcc ggtgtttcgt
     4921 cgtcaccgcc gcgaccccgc tacccggcgg tctcggctgg cgcgccgagc ggggcggccc
     4981 aggcgtcgta gccgtagacc cagtcggcgt tgccctggcc cttcatccag tcgttggcga
     5041 gcgagccgag ctggacgcgg gcggtgcggt cgcggcgggc gtcctcgtag cggcgcagcg
     5101 cgtcggggac gtcggcgcgc gtcgcgccgg cgagcgcgcg ggagaggacg acggcgtcct
     5161 cgatcgccat gcaggcgccc tgcgccatga agggcaccat cggatgcgcc gcgtcgccga
     5221 ggagcgtggc atggcccgaa gaccagcgcg gcatggggtc gcggacgtag agcgcggact
     5281 tggtgaccga ggcgacggcg ccgagaagcg cgccgacctc gggatggaag tcggcgtaga
     5341 ccgcccgcag ctcggcgaca tcgccgggaa gcgtccagct ctcctcgcgc cagtcgtctt
     5401 ggggcgtggt ggcgaagatg aagatctcct cgccgcgggt cagcgggaag acgacgacct
     5461 gcacgtcggg gcgcggaccc caccatttgg tgaagctgtc gaggttctcc gccgcgaccg
     5521 cgtcgcgcgg gacgacggcg cggtaggaga cgaggccggt gaagcgcgga tgatcctcgc
     5581 cgaagagcga tgcgcgcacc gccgagtgga tgccgtcggc gccgatgacg acgtcgaagc
     5641 gctcgctcga tccgtcggcg aaggtgacgg tgccgtcgga gacggccgtg accctgtggc
     5701 cgagccggat cgagctttgc gggacccgcg cctcgagggc acgcagcagg tcgccgcggt
     5761 ggatggtgag ctgcggcgca ccgtatttgc gctcggcctc gtcgcccatt ggcaggcgcg
     5821 aggtctcctc gccgctgtcc caggttcggc tgatgcggaa gcgcggccgg gcggcggtct
     5881 cgcgcagggt ctcgccgacg ccgagccggt cgagcgcgtg caccgcgttc ggggtcaggt
     5941 tcacgtccgc gccgatgcgc gcgaaggcgc gggcctgctc gtggacggtg acggcgtggc
     6001 cgtccttcgc gagggcgatg gcggccgtca ggccgccgac gccgccgccg cagatgccga
     6061 tctcaaggcc catgcgtatt ccccttgtca ggcgtaggtg tagccgaatg agagcgtgaa
     6121 gcggtcggcg gcgagcggcg ccgtgccgag ccgggcgcgc cggatccaga ggcggccgtg
     6181 gacctggatg tcgctgaagt ccgtgccctc ggcgaaggcg gtcgaatcgc accagatgcc
     6241 gccgaggtat tccgccccgc ggaagtcggc cggcgccccg aagtcggcat gggcgagcga
     6301 gacgttgccg taggagacgg tgccggcgaa gctcgcgcct tgggcgaagc gggcatggtc
     6361 gaagcgggcg atgccgcgga actcgacctt cgagaagtcg gccggcgcgt cgaagctcgc
     6421 gtcctcgaag cgggcgtcgg tcaggaactg cgcgcgggcg aaggggctcg ggccctcgaa
     6481 ggcgacgccg cggaaccagg cgaggccgag gaagcgggtg cccgtggcgt cgatgccctc
     6541 ggggaaggtc gcgccggaga aatccacgcc gggaatctcg cgaccggaga ggtcgagccg
     6601 cccctcgcag gtgacgccgg cgaggtcgac atgctcgccg tgcttccagg cgaccgacag
     6661 gcgttcgagg atctcggcgg cgcgcatcag gcggcctccc cgacgcgggt gcgggcgagc
     6721 tcgacgaccg catcggccca ttccatggcg aggtcgtcgg ccatgtcgga gccggaggcg
     6781 tcgtggatca cgcggtcgcc gacctgggcg gcgccgcggc ccttcagcat gtcggcgatg
     6841 atctggctgc cgaaattgtg ggtctcgtcg tattcggtgt cgccgaggcc gaagatcgcg
     6901 aaatggatgc cggcgagatc cggctgcgcc gtttccatcg cctcggcgaa gggcttggcc
     6961 gaggagggca actcgccgtc gccataggtc gagcagatca cgaggtagaa ctgctcgggg
     7021 gcgaagtcgg aaggcgcgaa gtccgagagg ttggtgcagg tgacctcgtg gtcgccctcg
     7081 aggtgcgcgg tgacatcttc ggccagcatc tccgcattgc cggtctcggt tccgtaaagg
     7141 acggtgatgt tcatggctta tccctcgttg ttgagtgcca gcgcgagcat ggccgcgcgg
     7201 tctgctatct ttctgcggca gcggtccgcg gtggcggcgg cgagctcggc cgcgtcgatg
     7261 cgcttccagc cggcgtagtc gacgcggccg gtggcgggga ggccgggcct gccggtcgcg
     7321 gcgtcgaggt cggcgacgac ccgggcggcc agcgcctgcg cctcggcgcg gttctccggg
     7381 atggtgccgc gcgggccgcg gcggaaccag ccggtggcgt agaggccgtc gtcgagcacg
     7441 ccggcgtcga tgtcgcgcgc atgctcgagc agcgcgccgc gcgagaggtt ggcgccggtg
     7501 tcgaagccga tggcgctgat gaaggtgtcg cagggcagca ccgtctcggc acccgcggcg
     7561 tcgtggaagc gcacggcggt gacccggccc tccgcgccct cgaaggcggt gggggtgagg
     7621 ccgaagcgga aggtgagcct gcggggcccg tgggcgatgc cgtcgatggc cgcgagcgcg
     7681 tcgagcttct tctggcccgc gggctccccg gcggtgccgg cgccctcgac cttgatcgtc
     7741 gcatgggcga ggtggtgcag ttccttgacc atgaccggat cgaacttggc atgcgacgcg
     7801 gccgagcggc cgacgatgtc gatggtctcg atatggccgg cggtgagcca ggcggtcggg
     7861 accggcccga ggtcggtgcc ggcgagctcg tcggcgctct tggcgatgag ccgcactatg
     7921 tcgatggcga cattgccgtt gcccatgacc acgacgtgcc ggccgaggac gggcaggggg
     7981 tccgcgtccg ggtgctcgtt gagcgcgcgg gtaacggtgc cggcgccggc gatgccggcg
     8041 agatcctcgc cggggatgcc caggcggcgg tcggtggcga ggccggcggc gagcacgacc
     8101 gcgtcatagg cggcgcggag ctcgtcgagg ctgacgtcct cgccgatgtc gaggttgccg
     8161 atgaagccgg cgccctggcg ctcgaagagg cgctcgaact ggcggatgat cgccttggtg
     8221 ccctggtggt cggcggcgac gccgtagcgg acgaggccgt agggcacggg caggcggtcg
     8281 atgaggtcga gcttgaggtc gggcttcgcc ttgagcagcg cctgggcgag gtagcagcca
     8341 gacgggccgg agccgacgat ggcgacggat ttctgcatca ggcggctccc gcccaggcga
     8401 ggggctttcc ggtgaggtcg gtatagatcg acttctgcgc catataggcg cggatgccgt
     8461 cgcggccctt ctcgcggcca acgccgctct ccttctcgcc gccgaaaggg gtggagatgg
     8521 agaactgctt gtaggtgttg atccagacgg tgcccgtcct gagcgcccgg ccgatgcgcc
     8581 aggccctggg gaagtcgcgg gtccagatgc cggaggcgag gccgtagtcg ctgtcgttgc
     8641 cttgcgcgat cacgtcggcc tcgtcctcga aggggaggac gacgaggacg gggccgaaga
     8701 cctcctcgcg gcagatggtg gcggaattgt cgacgccggt gaggatcgtc ggcaggtaga
     8761 aggcgccctt gtcgtaggtc tcgcccgccg gggcgcggcc gccggccagc acctcggcgc
     8821 cctcggcgcg ggcggcgtcc accatctttg cgaccgaagc gcggtggtcg aaatggacga
     8881 gcggcgcgac ctgggtcgcg gggtcgaagg ggtggccgac gcgcaggcgt tcggtcgcgg
     8941 cggtgaggcg ctcgacgaag ctgtcgtaga tcgggcgttc gacgaagagg cgggcgccgg
     9001 cgatgcagct ctgccccgag gaggagaaga tgccgtagag gacgccggca atcgcctggt
     9061 cgatgtcggc gtcggcgaag acgatggtgg gggacttgcc gccgagttcg agcgagaccg
     9121 gcatcagctt ctcggccgcc ttgcgggcga gcgcccggcc ggtggaggtg ccgccggtga
     9181 agctgacctt ggcgatgtcg gggtgctcga cgagcaggtt gccgatctcg cgcccggcgc
     9241 ccggcagcac ggagaagagg cccttcggca ggccggcctc ctcgacgatg cgggcgagct
     9301 cgaggctgac gagcggcgac caggaggcgg gcttgagcag caccgcgttg ccggcggcga
     9361 gcgccggcgc gaccttctgg gcgtccgagg cgatcggcga gttccagggc gtgatcgcgg
     9421 cgacgaggcc gagcggctcg tggaccgaca tggtcagcgc gtcgccgcgc tgggtggtca
     9481 gcgcctcgtc cgcggtctcg agcgcggcgg cgaaatagcg gaaggtgccg gcggcggagg
     9541 cggcgagcgc gcgggtctcc ttcagcgtct tgccggtgtc catggtctgg atccgcgaga
     9601 tccggtcgac gttcgcctcg atgccgtcgg cgatccgggt gaggatgcgc gcccgctcgt
     9661 gcgggcggag gttgcgccag gccgggtcgg cctgggcgcc cttggcgcgc tcgatggccg
     9721 cgagcccgtc ggcctcggag gcgcccttca gcacccggtt gaccgagccg tcggcgggaa
     9781 agatcgaggt gatctccgcg ccggagccgg tggtccattc gccggcgacg tagagcttgc
     9841 cgtccggaag gtcagtcatc gcttctcccc acatgaaact tcctccctca gatggcgacc
     9901 gggccgaggc ggttccggat ttcccgcagg acgctgtaga tcgtcgtcgc cgaggtcttg
     9961 gcattgccgg acgacggctt gttctcgatg cggatcgtgt agttcgcgat cggcgagacg
    10021 acggaatatt cgtggacgtt gtcgggggcg gaaggatccg ccatcagctc gacccgggtc
    10081 gcctcgaagc ccgccccggc gagcgccagc gtcgcggcga cattggcgtt cttcggatag
    10141 gcggtcgccg cctcgcgcgc gttgccggtg aaaaagatcg ccggctcggc gagcgacgcg
    10201 aggtcgaggt tctcggcggc cggcgtgccg gcccaggcgg cgggcggctt gatgccgcga
    10261 tagcggacct cgagcccgcc cccggcgccg agcgccgaga ggaggtcgat cgcgcccacc
    10321 gcgccggccg gcaggatcag gcgtgcgccg ccctctgccg ccgcggcctt gagcgcggcc
    10381 tcgagcgccg ggtcggcgag tgcgccggtc gagacgagga cgacgtcggt gccggcgcgg
    10441 agcacggccg gcgcatgcgc ctcggccgcg gaatggcccg cgcattcgac gacgaggtcg
    10501 ggccggtcgg cgagcaggcc ggcggcgtcg gagaccagcg cgtggctccg cgccgccccc
    10561 ttgagctcgc cgtcgaggcg gctgcgggcg gcatccgccg cgcccggccg cacgagcacg
    10621 atcaggtgat cgagcggggc gccctcggcg atcaggccga gcagcgccgt cccgatgttg
    10681 ccgaagccga tgagaccgag gcgcatggcg tcagaccttg tttccggcgg cgccggcggg
    10741 cgggccggag aaggattcgg cgaaggggcc gatcgcgacc atgtcgacct ccacgacctg
    10801 cgggccgtcg gcggcgagcg ccgcgtcgag cgccgccggg aagtcctcca ccgagcggac
    10861 gatggtgtgc ggcatgccga tggcgcggca gaaggtcggg aattccggca ccgcgacctt
    10921 ggagaaatgc cgccggctgt cgtactgcgc gtcctggatg ttctggatca cgccataggc
    10981 ctgatcgttc atcagcacgt agaccagcgg cgcgttctcg tcgacggcgg tgatcatctc
    11041 cgcgaggccg agcatggtgc cgccgtcccc gagcagcgtg atcgccttgg gtcccgacga
    11101 gccgagcgcg gccccgacgc ccatggcgac gccctggccg atgccgccgc cgagcgcatg
    11161 cacgccgtgg cgcgggttgg cgatgcgcac gtagcggttg ccgaaggtcg agttcgagat
    11221 cgtcacgtcg cgcacccagg ggtggcggcc ggcggcgacg cgctcggtca gggcctcggc
    11281 gacgccggca taggggccga gcacgttgcg catccggccc tccgactgca cgcgggtacg
    11341 ggcgatgtcg aagggcaggt tcggatcggt gtcgagcttg gccggaagcc ggttgagcaa
    11401 ccgccgcagc gtgtcggtgg cgtcgccatg ggcgaagatc tccaccgggt agttgcggcc
    11461 tccctggcct gcgtcggcat cgatctggat cagcggccgc ggcagcggca tctggttgtt
    11521 gcgcgtctcg ttgccgcgca gccgcgagcc gacgacgatc atcaggtccg agctcgcata
    11581 gagctcggcg gcttcgggcg tcatgttgaa ggcgccgaga ttgcccggct cctcctcgga
    11641 gaccgtggcg cggccgttcg tggaggtgac cacgccgaag ccgcgcttga gcagttcggt
    11701 cgccggcgcc ccggcgccgc gggcgccgcc gccgagccag agcagcggcc ggcgcgcctt
    11761 cttcaccagt tcggcgatct cgtcgaggac cgcgtccgag gcgatcggcc gggtcgcgcg
    11821 gggggcatgg atgcgggtgg tgccgtgggc ggtcgagcgc tgcacgtcga ccgggatctc
    11881 gaggctgacc gggccggtcg gcgccgagag ggcggccgag accgcggcgg tcagcgcgcc
    11941 gatggcgccg ttcgcgtccc acatgcggaa gaccgccttg gagacgccgc gcagcatttc
    12001 gggctggcgc ggcacgtcgt ggatcgcggc gcggtcgcgg tcggcgaatt cgcggtcgac
    12061 ctgggtggtg atgtgcagga ccggcgagcc ggcggtcagc gcctcggcct gggcgccggc
    12121 ggcgttgccg gcggcggtgc cggtcgaggt catgcagacc ccgaggccgc cggtgacgcg
    12181 ggcataggcg tcggccatgt tcatggcgcc ggcctcgccg cgggcgggga cgaagcggat
    12241 cttgccctgg cgcgccacgg cgtcgaggat cggcatgttg tggatcgaga tcacgccgaa
    12301 gatggtcgtg acgccgatct cggcgaggaa gcgggcaatg aagtcgccga cggtctcgat
    12361 gccggcggcg gtgtcgccgg gggtttcggt gtcatcgatg gtcgcgagaa gcatggtctg
    12421 gtccctcaga tatgacgcgc gaggccgccc gagacgtcga gctgggcgcc ggtgatgtag
    12481 ctggcggccc tggagccgag gaaggcgatg gcggcggcgg cctcgctcgg gtcgccgaga
    12541 cggccgagcg ggatgccctt gcgggcggcg agattgccgt accattcggc gcggctctgg
    12601 ctctggtcct cgcgctcggc gaagcggcgg gtccattggc cggattcgac caggccgagc
    12661 agcaccgagt tgacccggat ctccggggcg agctccacgc tcagcgattt cagcaggttc
    12721 tgcaccccgg cgcgggccga ggaggtgcag accatatgcg gctccggctg gtaggcgagc
    12781 agcgagttga ccgcgacgat ggcgccgcgg ctttcgcgca gcagcggcat gaaggcgcgg
    12841 gtcggatgga tctggctgaa gagcttcagc tcgtattccg tgcgccagtc gtcgtcctcg
    12901 gtctcggcga aggaggagac gcggccctgg ccggcgttat tgacgaggag gtcgcagcgg
    12961 ccgaaccggt cgcggacctc ggcggcgaag gcatcgaccg acgcgcgctc gagcaccgag
    13021 agggcgcggg cgaggacgcg ctcctggccg tggtcccggg tgaggacgca ggcgacgtcg
    13081 tgcagccgcc cctcgttgcg ggcgcagaag gcgacctgcg ccccgtgctc gagcaggagc
    13141 ctgacggtgg cgaggccgat gccggaggat ccgccggtga cgaccgcaac ctttccctga
    13201 tagccgtagt ccatcacctt gtcctcagtt cggggaagcc gggatcgggc cggccctgca
    13261 tcacggcgcg ctgtgccggg gtcggggggc cggcggtcat ccagcggtcc atcgcctccg
    13321 gatcggtgcg cgaccagacc ttggcctcgt gggtcgcctc gtcgatctgc tgcagctcgg
    13381 aggtgaactc gatgacgaag cccgagggcg agacgaaata ggcgaagggg ttgttgcccg
    13441 gcccgtggcg gccgggaccc caggagggga cgtggccctt ctgcttcatc cggccgatgc
    13501 cgcgcatgaa ttcgtcgagc gtcggcatct cgaaggcgac gtggttgacg gaggcatagt
    13561 tgccgcggac cagcgcgatc gaatggtggt cggagttgca gcgcaggaag accatctggt
    13621 cggcggaata atccgaggcg cggaagccga gcacctcctc gtagaatttc tgcgttccgt
    13681 ccatgtcggg ggtgttgagc acgacatggc tgaccttctt cggcttcgcc cagtccttct
    13741 ggtcgtcgag cacgcgcgcc tcggtgcgga agcgcaggcg ccggttgtcg gggtcgagca
    13801 tctcgaagcc gtagccgccg gcccagtcgt cgaacgcgcc gggggcagag atcggcgtgt
    13861 ggcccctggc gatgatctgg gcgtgcagcg cgtccatgga ggcacggtcg ggcatggcga
    13921 aatggacgta ttcgatgccg aagaccggcc cgtccttcag gccgtagagg aagggctcgg
    13981 tgccggtgcc gcgcaggagc gcgacgccat cttccttgtg ggagaggctg aggccccaca
    14041 tgtcctcgta gaaggggagc gtcgcggaga cgcccgggcc gcgcatcatg atgccgcgca
    14101 gcgatccgac tcggacgggt tcggtcatgg ttcgcgtcct cctgttcgtc ccggctcagg
    14161 cgaccggcgt tgcttggggg gccacggctc gcgcgaaggc gaggggggcc tgggtgtaga
    14221 gcgcgtggcc ggcggcgggg acctcggtga ggcggccgcg gaccagggcg gggatggcgg
    14281 cgtggagggc gcgggcgccc tcgggcgggg tcacgatgtc ctcctcgccg acgatcacgt
    14341 cggtgggaac ggcgaggcgg gcggcatcct cgagcagccg gccggaggcg agcatgcgcg
    14401 aggcctgatc gtagccgggg ctccggacct tcgacatgcc ggcctcgacg gcggcgacga
    14461 gcttgcggtt ctcctcgggc cggtagacca gccgcgcggc gcggacacgg gcgaactcct
    14521 ccgggccgag ccgggcgagg tcgtcgatgc gcgcctgggc cgcggggctc agcgccccgc
    14581 cgggggcgac gccgtggccg agcgccggcg aggcgagcag caggcgctcg acgcgcgccg
    14641 ggcgggcgac ggcgaaggcg gcgcccatca gcgtgccgag cgagtggccg acgagggtca
    14701 cgcggtcgag cccgagccgg tcgaggaagg cttcgagcgc gtcggcgtag tcggcggcga
    14761 ccggccagtc ggggccgagc ggcgcggagg caccgtagcc gggggcgttc caggcgacca
    14821 cctgccggtc gggcggcagc agcggcagga gctccgcgaa ggaatcggcg ttcgagccga
    14881 tgccgtgcag cagcaccagc gacgggccgc tccccggccg ctcgcggtag tcgatgcctg
    14941 cggaggtctg ggcggtgaac atgcgcgggg tcctcaggcg aagacgaagc cgttgttgac
    15001 cggcaggagc tgcccggtga gcgtcagcgc cccctcggag aggaggaagg cgatggtgtc
    15061 gacgatctcg cccggcgcct gcggtccggg gacggcgcgc ccgtcggaat agagctggtg
    15121 ccgggcctct ggcacgtagt cggtcgagga ggtcgtgagg atgccgggtg cgacggccgt
    15181 gacgccgacc cggtcggggc cgagctcgcg ggcgagcgac cgggtcatgg agatcaccgc
    15241 gcccttcgac gcggtgtagg acaggagtcg gggtgcgccc cagatcgcgg tgtcggaggc
    15301 gatgttgacc acgcggcccg agcccgaggc gcgcaggagc ggtgccgccg cgcgggtcat
    15361 cagccaggtg ccgcgcacgt tcacccgctg cacccggtcc cagctctcga tctcgatctc
    15421 ctcgaagggg atgccgccga tgcccgtggc gatcgcgccg ttgttgacga ggccgtgcag
    15481 cctgccgccg gtcgcttccc cgaccgtcgc cgcgagcgcg gcgatcgagt ccgggtcggc
    15541 gaggtcgacc ggcgcgaagg tcgcgccgag cgcctcggcc gcggcgcggc cctcgtcctc
    15601 ggcgatgtcg gcgaggacga ggcgcgcgcc gtggccggcg agcccgcggg cgatctccgc
    15661 cccgagcccg cggccggcgc cggtgacgag gatgctgcgg cccgcgagca tcactcggcc
    15721 gcctgggcct gggcgagttc cgcctcgacc tgcgacttgg cggcacgggt caggatctgg
    15781 cggatgcggc tgacgccgag gtcgtgctgg tagagcatct cccgcttgcg ggcgtcgtcc
    15841 ggcatgccct cgagcatgac ccggtcctgc tcgagcaccg cccagtggtt cggctcgagc
    15901 accgcccggt agaggaagcg ccagctgtcg cgggcgacgc ccgagacctc gcgcatgcgc
    15961 cagaagaaga ccttgcaggt atgctcgtcc accgggagcg tgaagccgat gatccgcatg
    16021 aagccgcccg ggcccgcggc cggcggatag gggatgtcga ggaagcagaa catgtcgccg
    16081 gcgtggatct cgaactcgga ccagtcgaag ttctcgccca cctggccgac gcggctgatg
    16141 tggaagccgt tttcggtctt gtcgagcttc atcaggtcct gcttggagcc ataggccagc
    16201 gtgaagctct ccgaatgcag gtagcagccg tgcatcgggt cggcgaggtt gtcgagcgcg
    16261 tagcggtagt tgcactccca gaccgaggtg cagaggaagc cggtccaggc gtcgctgacc
    16321 agctccttcg gcagcacgag cgcgggcggc tcgggctgct cgaccgaggg gaagtagacg
    16381 aagacggcgt cgttcgactc ggtcgtcgcg aaggacttga gcgccttgcg gccctcgagc
    16441 gcgcattccg gcatcgccgg gacccggacc accacgcctt cgccgttgac gatgacgccg
    16501 tggtagcggc agccgatgtt gccctcgtgg acctcgccgt aggagagcgg cgcgccgcgg
    16561 tgcgggcaga agtcctcgat gcagttgatc ttgccgctgg cgtcgcgcca gagcaccagc
    16621 ttctggccga gggccttcac gccatgcgga cgggtgccct tcacctcgac cgacttggcg
    16681 accggatacc actggccgag gaggccggta ttcacccgct cttcgatgag cgtcttcttg
    16741 tcgatggctg tcatggctgt ctcctgcggc ctcgggtctg cggggggcac cgggcgcccc
    16801 ccggcgcaag tcacttcttg tcgcgcttga tctgcgagac ggggtggtcg ggcggatagg
    16861 tcggcgtgac cggcttcggg ttgccgatga gcacgcacat cagcgcctcc tcgatgccct
    16921 cgttgcgcag gccgcggtag aggcccggcg gcaccgagac catgtcgcgc tcggagagca
    16981 cggtctcgac cagctcgccc ttgtgctcga tgaagaagcg gaccttgtgg cccttgagga
    17041 tgaagaacac ctcctcggcg tcgcggtgga tgtgcagcgg gccctcgcag ccggccggca
    17101 gcaccatggt cgagaaggtg aagttctccg ccgggatcac gttctcgtcg tcggtgacgc
    17161 cggtggcgcc ggtgccgatg tagcgcatct gggcgcggcg gtagatcggg tcgtagtccg
    17221 cctggaactt gagcgcgtcc cagtccagcg tgcgggtcgc gtagcgggcg gtccggctct
    17281 cgatccagtc gccgaggctc ttgccctcgg ggatcatcac gtcttcgggc tcgatcgacg
    17341 ggaacttctt cggaacagcg gacatggtcg tctcctgtgg aaccaagtgg ttgggtggtt
    17401 gccggcccta ggtcagaact gtgccttggg caggtagtgg agcaggcggg tctcggcgcg
    17461 gacgagcgcg gagtagagca ggaagccgat gacggtcagc aggatgatcg cgttgaagac
    17521 catcgccgca ttcgcctggc cgccgccgta ggagatcagg aagccgaggc cggtgttgcc
    17581 accgacgagt tcgcccaccg tgacgccgat gaccgcgagg gtcgaggcga tgcgcaggcc
    17641 cgccatcagg ttcggcagcg tcgagggcat ctccactttc agaaagatct ggaaccgcga
    17701 gaggttgtag gcccgcgcca ggttcaccag gtcgcggtcg acggtccgca tcgcctgcag
    17761 cacgttgacg agcaccggga agaacacgat cagcaccgtg accagcagct tcggcatggc
    17821 gttgtagccg aaccacatga tgaagagcgg cgcgaaggcg accttgggcg cgatctgcag
    17881 cgccaggata tagggcgaga gcaccttctc ccagaactcg gacatgccga gcagatagcc
    17941 gagcgccgcg ccgagggcgc tgccgatggc gaagccgagg atggtgtaga gcgcggtcga
    18001 ggcggtattg gcgagcaggc tctcgctcgt ccacatccgg ccgaactcga cgaggcagtc
    18061 cgagagcgtg gggatgatgt acttcggcac cgccagggcc gtgggcagga tctgccaggc
    18121 gacgagcagc agcgcgagca acccgaggct cgcatagagc gcggcgcggg actcgcgggg
    18181 gcgggtcgtt gcggcggcgt tcacggccgg ggcggttgcg gtcgcgctgc tcattgcacg
    18241 aactcgttgg tgtagaggtc ctcgaccggg atggcggtct gcacgacgcc cgcgtccatg
    18301 tagaaatcct gcaccgcctt gatccgctcg ggatcgaagg tgccgagcgg ctggccttcg
    18361 gcctggggat agaccttcgc ggcataggct tcgaggatcg cctgcacctc ggcctccttg
    18421 ccggcgtgct gcggcaccgc ggcgacgaag tcctgcgcgg cctgggccgg gtcggcctcg
    18481 atgtcgcgaa ccgcgtgcag gacggcgttg acgaagccct tcaccacctc gggccgctcg
    18541 gcgatcgtcg cgtcggaggc gatgatcgcc tgggccatcg ccgggaagac ctcgtcgacc
    18601 ggcatctgct cgacctcgac gccgccgccc tcgaccgcgg cgatccattc cggcacgccc
    18661 gacatcgcgt cgagattgcc ggcgatcatc agctggatga tgccgcccgg gccgacgccc
    18721 tggatgttcg cctcgctctt gtcgaggccg gcggaggcga gcacgccgag caggttgtaa
    18781 taggtggtgt cctggaagga gagcacgccg acgttcttgc ccttgaagtc ggcggggccg
    18841 gtgatgccgc tgtccttgcg ccaggccagc tgggtcaggc cgcgaccgcc gagcagcgcc
    18901 acgccacgga tgtcgaggcc gttggcgcgg acgatgatcg gcgtgtcgcc gatgccgccg
    18961 cccatgtcgg cgttgccggc ggcgacctgg gtcgcgacgt cggcgccgcc cttgccgacc
    19021 tggaaggtca cgtcgagccc ggcctcgtcg aagtagccct tggccttggc gagttggaag
    19081 ggcgcgaagg cgggcaggaa gtccggggcc gggaagaggt aggtcacctc ctcggcggca
    19141 tccgccgcgg cggcgaaaga gatcgcggcg gccgcgacgg tggtcaggaa caggcgcttc
    19201 atgtcgtcgt ccccctgttg ggaatggctg tcgtcagatg gctttttctt cgagcttcag
    19261 gtgccggaag atctggccgg cgtattcgcc gacctccggc atcacccgcc gcttcaaggg
    19321 gtgggcgcgg tgcggcagct cgaccttgat ctcggtgagc accgtgcccg gccgctcgga
    19381 gaggacgagg atgcggtccg acatcagcac cgcctcggag aggtcgtggg tgatgaggag
    19441 ggtggtcttg ccgctctcgg cgatggtcgc ggcgaagctg tcctgcagga ggagcttggt
    19501 ctgggcgtcg agcgccgaga agggctcgtc gagcaggatg atctcggggt cgatggcaag
    19561 cgtgcgtgcc agcgccgcgc gctgccgcat gccgccggag agctggtagg gatagtggtt
    19621 ctcgaagccg gcgaggtggc agcgcttgag ctcggccatc gcccgctcgc gccgcggtcc
    19681 gggggcggcg ccgcgcgcct cgagcccgaa ctcgatgttc ttcacgatgt tgcgccaggg
    19741 cagcagcaga tccttctgca gcatgaagcc gacgtggtcg ttcggcccgg tcaccggctg
    19801 gtcgctgacc agcacctcgc cggtcgtcgg ctggtagagg cccgcggtca tcgacagcag
    19861 ggtcgacttg ccgcagccgg acgggccgac gacggagacg aactcgccct cgttcacctt
    19921 gaaggtcacg tcccggatcg cggtcagcac ggcgcccgag ttcgtggtga actgcttggt
    19981 gacgccgcgg aactcaagcg ccatagccat gctccgcata ggccgcatcg agctcgccgt
    20041 tgattcccgc cagttcggcg gtgagcagcg cctcggtcca gtcggtgcgc cccgacatcg
    20101 gcgccgtgac ccccttcgcg gcgagcgcct cggccacggc cgcatagtca tgcgtgccct
    20161 cgccgtagat cgccatcagc gcctctgcca gcgcagcctc ggcgggcgtt atctctcggc
    20221 cgcggcactg gtgcgccagg gcgggacggc tggtgtcgcg agcccgctgc gaaagccgat
    20281 ccttgaaagc gtccacctcg ctgacctcct tcagagcatg tgttcacaaa tgaactatgt
    20341 gttcacatct tatcgcccac ggggcggaga cttgtcaatc gttaattcga accttggacg
    20401 gagcgccggg cgccgcgcga aagccgcgcg ccgcaaacgc ttgcatattc tcggcaatag
    20461 ccgggagctg tggtgttcaa gtcgttcggt tcgggtgcgg cgcctcaccg gcgcacggcc
    20521 tttgtcgtgc ccggcgccgc cagggggggc gttgcgagct agaccctacc cgaatcaagc
    20581 agcgaagccc atgtgtctgc ctcggcccgg gacccatgcg accgcgcggg tgttggaggg
    20641 agtggcattg gtcgggtgcg cgttcatggg agcgagcccg gacgcagccg acgatcgatg
    20701 gctgcgcgaa gcgtgcgatg gtcgggtgtc gcccgcgtgt ttcgccgccc tgaaggccca
    20761 gcggatggcc aggccgtgac gatagaggtc tgggccgggc acggcagcat gccgcaatca
    20821 tgggcgcggc ggcgcccggc gccccgggca aggaaggcgc gggacccctc gtccgaaaac
    20881 cggagcgccg aggatgacga cgacgaggcg aacgacgtgg tgggtgacga cgaaggccat
    20941 gtcggcaccg gacacgatcg caaggaccac catctcgccc tggccgccgg gtgcgaagga
    21001 gagcagggcc tcgaccggct cggcgaggcc gaggagaacc gccgcctcgg cgaagagcgc
    21061 ggagatcccg gtcaggatgg cgcaaaagcc cagcgcagcg agcacgaccg tgcggagttc
    21121 ggcgagcgtg atgccgcgat acttcgcacc gattccgagg ccgatgaaga actgggccgc
    21181 aaggatcgct tccgcgggag ggcgggcgtg gatcaggccg gcgaggctgg cggcggcggc
    21241 gaggatcatc gggccgagga tcgcggctcc gaacaggcgg gcgcgacgcg ccagccacca
    21301 acctgcggcg gcgcagaaaa ccatgatcgc gagctcgcgg gccggaaagc cggccgccgg
    21361 gagaccggga ggggcgtcga gggttcggcc ccagatcgac gaaacgagca gcggcatggc
    21421 tgcgacgatg gcgaggacgc gcgtggcgtg cacgaggctg agcgttcgcg ggtccgcccc
    21481 tgcctcctcg ccgagcagca ccatgtcctg gaaaccgccg ggcatggccg aataataggc
    21541 ggtcggcgcg tcgaagccgc acagccgccg gaaatacggc acgccgacga ggccgatcag
    21601 cacgaccatc aggggcacca tggcggcggt gagcgccatg tccggaagcc gcgtgatgag
    21661 ctcgggggtg atcgaggcac ccacggcgac gccgagcacg gtgcgcatgg cgtcgccaat
    21721 tccgggcggg gcggtaaccg gcatcccgcc caaagatgcc aggagcccgg cgcacatcgg
    21781 cccgagcagc cagggcagcg gaaggtcgag ggcgccgaag acgacaacgc ccccgcccgc
    21841 gaggagcagg gtcgctccga tgcggacggg ggcggtgcga agcatcggcc cggggatcaa
    21901 tcgacggagg tggcgccgcc gggctcctcc tcggccatgt gcatgcggcg cttgatgcgc
    21961 ttgccgacca ggatcggcag cacgaagccg acgacggcga tggtccagag gatgatggcc
    22021 aggggcgagg taaagagcac ggtccagtcg ccatcggaga tggtcatggc ccggcgcagg
    22081 ttcgcctcca tgaggttgcc gagcaggatg ccgaggatga ccggcaccag cggcacgtcg
    22141 agcttcctca gcacccagcc gagcacgccg aagatcacca tcaccaggag gtcgaaggtc
    22201 gagccggaga tgccgtagat gcccacgaag gagatcatcg cgacgatcgg catcaggaac
    22261 ttcggcggga tcatcagcac ccgtgcgaag agcccgacca tggggatgtt catggcgagc
    22321 agcatgaagt tggcgatgaa gagcgccgcg atcaggcccc agaccacgtc cg
//
LOCUS       DAOTFI010000045        22242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2771_length_22242_cov_7.312210, whole genome shotgun sequence.
ACCESSION   DAOTFI010000045 DAOTFI010000000
VERSION     DAOTFI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22242
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_22242_cov_7.312210"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..685
                     /locus_tag="P5558_06400"
     CDS             362..685
                     /locus_tag="P5558_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY23973.1"
                     /translation="MSASSARRPARETGAFCKLSGLIERALFEWRPADVEPYVLHFLD
                     CFGPERLMFASNWPVSNLHGTYARWWEALQEILDRQGLGVDERRHLFETTAVRAYRLP
                     PARPA"
     gene            complement(717..1562)
                     /locus_tag="P5558_06405"
     CDS             complement(717..1562)
                     /locus_tag="P5558_06405"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY23974.1"
                     /translation="MSTTAAFDDHRPGRARLSAAEMQAYRLDGLVKPAHGLGAAELAG
                     LRGLLEETLAATPGQRPESLVCPHIEGMGNLPAAITDRWLAVCTRADFLDLVEQVLGP
                     DVILWGSQIFCKPAGTGLRVPWHQDGHFWPIRPLATCTLWLALDDVDAENSAMLYVPG
                     SQRTRTLFPHADQPSEESALNAEMLPGDVDLDRVEVDALPAGACSLHDVYLVHGSEPN
                     RSARRRAGFVIRYMPATSHFDRELAISTSPQAATKFAERPIYLLRGEDWTGKTGLVDL
                     RDRPA"
     gene            complement(1668..2144)
                     /gene="nikR"
                     /locus_tag="P5558_06410"
     CDS             complement(1668..2144)
                     /gene="nikR"
                     /locus_tag="P5558_06410"
                     /inference="COORDINATES: protein motif:HMM:NF002815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HRY23975.1"
                     /translation="MRRVTMSFDDELLAELDAFLQKSGAASRSEALRDLVRRGLGQVA
                     LDGKATGDCVGVVSYACHQSMRGLARRLAESRHDLHDRTIATTTAPLDHDTAIEVTIM
                     RGDARELHGHAESLFLERGVMHGNVSLIPVEPQRDTHAHGPGRPRAHLHLKVRESF"
     gene            complement(2234..3121)
                     /locus_tag="P5558_06415"
     CDS             complement(2234..3121)
                     /locus_tag="P5558_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY23976.1"
                     /translation="MATLNFHHLRYFWAVAHEGNLTRAAERLNLSQSAVSIQIRKLED
                     ELGHALFERRGRQLVPTEAGRIALDHADAIFAAGDELLDTLAESGRGRRVLRVGALAT
                     LSRNFQIAFLRPLLGRDDVEIVLRSGGLGDLLASLEAHRLDVVLANATPPGDAATPWR
                     AQPIAEQPVSLIGTPRRLGEDRSLEVLLQREPLVLPTAQSGIRTGFDALADRLGIRPR
                     IAAEVDDMALLRLLAREDIGLAVVPPIVVRDELASGRLREAVQLPRLSETFFAVTLRR
                     RFPNPLLRELLGAAAAEQG"
     gene            3249..4850
                     /locus_tag="P5558_06420"
     CDS             3249..4850
                     /locus_tag="P5558_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRY23977.1"
                     /translation="MPSVLALVLFLAPLSLVAVAMLARREPGSRPRRVLRAAELATLL
                     TLGMAVLAGLAVALAGPGTSPVLGLGGIGLAIRLDALSVLIFGLVAFVGAVVVRFSRN
                     YLDGDGRQGAFIGGLCLTLAAVSLLVLAGNLGQLVLGWVLTSLALHRLLVFYPDRRGA
                     VLAARKKFVAARIGDACLVAAVVLLLRAFGTADIGTMLEAARLGGATLPPTGVATAAV
                     LLAFAALLKSAQFPTHGWLIEVMETPTPVSALLHAGIVNAGGFLVIRFADVMLLAPGA
                     LHLLAVVGCCTALVGATVMLTQTSIKLSLAWSTVAQMGFMMLQCGLGAFPLAVLHIVA
                     HSLYKAHAFLASGSVIGAAPAPTAEGPRPSRALASLGTALGLYLGLAWLFGITPAAEP
                     VRLALGAILAMGLSVLVMPAVTGPFDARLLGRTLGAAAGVTVAYLAVQAGAHLLLAST
                     LPPRPGPSTATHVIIGLALVAFAAVTAMQAIAPSLALRPGWQALRVHLANGLYVNAAV
                     NRLAGALRRPATVAPPSILQGNHSS"
     gene            4847..7345
                     /locus_tag="P5558_06425"
     CDS             4847..7345
                     /locus_tag="P5558_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HRY23978.1"
                     /translation="MISQPPIEDPSRAEILAAALRATRRIAPLWPLQGFVAVNPFLGL
                     ADHRFAEAARAMAASAGARLTMPRAFYAKAIRGGRITALDLADALAEAEDGGDLPADG
                     EALRAAALAPDPDVAPRPLPTVADVAGAATGRDWAGFVTDRLSAWAAAFFDAGQAAWR
                     SPWRDLGPYAAWRAEALVDRTPETMGLAGFRRAVEGLPGDASDALEVATRRIGMPAAA
                     LEAYGHRLLMTIGGWSGHARQRVWESELHGRPDASLLELLAIRLAWEAALLDCLADAP
                     AVEMAWHAALGAYATSPASHPALAVDCLLQLAYERAWQRELAARFRARPEAAPAERPA
                     VQAVFCIDVRSEVFRRALERAAPEVETIGFAGFFGLAIDYVPFGEMAGRAQCPVLLRP
                     GVTIRETVRDAGPAEALGLLACHRLRRRAAGAWSAFRQAAVSSFAFVEAMGLTFLGRL
                     ARDGLGPALPASVAAGGGDPVLAGRLEPSLEPGSLAGRATGIDIEGRVRMAAAVLEAM
                     GLRDGLARLVLLAGHGSTTTNNPYGSGLDCGACGGHAGEANARVAARLLNDPSVRAGL
                     ALRGIAVPADTVFVGGLHDTTTDALRILDPRDVPASHAADLARLVAWLAAAGELARGE
                     RAPALRLSTDRPIDAQMRARSRDWSEVRPEWGLAGCAAFVAAPRHRTRDIDLGGRAFL
                     HSYDWRRDEGFRTLELVMTAPMVVASWISLQYYGSTVDNRVFGAGNKVLHNVVGRLGV
                     IEGNGGDLRAGLPWQAVHDGERLVHEPLRLTVVIEAPITAINEVIARHGTVRQLADHG
                     WLHLLALDDDGLIGQRYVGGLEWAPMRAAMAEAA"
     gene            complement(7364..7795)
                     /gene="cueR"
                     /locus_tag="P5558_06430"
     CDS             complement(7364..7795)
                     /gene="cueR"
                     /locus_tag="P5558_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HRY23979.1"
                     /translation="MNIGEVASASGVSAKMIRYYESIGLLPPAARSESGYRVYGPNDV
                     HRLAFIRRARRLGFSVAQIGRLEALWRDTNRASAEVKRIALEHVAELEEKIAELEAMR
                     RTLRHLADHCHGDQRPDCPIIDDLAGAEPADRPANRSQTRA"
     gene            complement(7805..8329)
                     /locus_tag="P5558_06435"
     CDS             complement(7805..8329)
                     /locus_tag="P5558_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY23980.1"
                     /translation="MSLAGAGRHDPGMRIEPGDLAEPAVRSLLGALDAYLATLYPPAS
                     NHGLTMDALRQPAVRFVVARLGDAPVGCGAVVLEADDAEIKRMYVAPAARGRGVGRRL
                     LDHLESLAMASGCRTSRLETGIAQPEALALYRRAGYMDIGPFADYRPDPQSRFMGKPL
                     HGIEAVLGRAGSFP"
     gene            complement(8326..10686)
                     /gene="clpA"
                     /locus_tag="P5558_06440"
     CDS             complement(8326..10686)
                     /gene="clpA"
                     /locus_tag="P5558_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HRY23981.1"
                     /translation="MLSRTLEKTLRRALALANARQHEYATLEHLLMALTEDQDAVAVM
                     KACAVEIDKLRLGLTDFLDHELSGLVVDGVVDAKATAGFQRVIQRAAIHVQSSGRSEV
                     TGANVLVALFSERESHAVWFLQEQNMSRLDAVNYIAHGIAKKPSLSEERRVKGAEEAS
                     QGEEKREAASEALSTYCVNLNDKAAAGKIDILVGRQHEIERTIQILCRRSKNNPLFVG
                     DPGVGKTAIAEGLALRIIRGEVPDVLKNAIIFALDMGALLAGTRYRGDFEERLKAVIG
                     ELEANERAVLFIDEIHTVIGAGATSGGSLDASNILKPALAGGALRCIGSTTYKEFRNY
                     FEKDRALVRRFQKIDVPEPSEDEAVKILQGLRPSFEEHHRIKYSNEALKAAVKLANRY
                     IHDRKLPDKAIDVIDEVGAAQMLKPESQRRKTVGPKDIEEIVAKIARVPIKAVNARDK
                     DALRTLESDLEGVVFGQGRAIQALASAIKLSRAGLRDPQKPIGCYLFSGPTGVGKTEV
                     ARQLAAIMGIEIVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTDAIDQHPHAVLL
                     LDEIEKAHPDVFNILLQVMDYGKLTDNNGKTVDFRNVILIMTTNAGASDMSRPVMGFG
                     GIAREGEDTEAIKRLFTPEFRNRLDATIPFAGLTPSIIRSVVDKFVQQLAAQLADKRV
                     AIELDEPAKSWLAERGYDPLYGARPLARVIEEHLKKPLADELLFGRLAKGGKVLVTIV
                     DDKPSFEVLATTKGAPLAVLNDEDESPEASPSGLDTPDAELPETVG"
     gene            complement(10860..11183)
                     /gene="clpS"
                     /locus_tag="P5558_06445"
     CDS             complement(10860..11183)
                     /gene="clpS"
                     /locus_tag="P5558_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRY23982.1"
                     /translation="MSDGNNEERIGKGDVATKTRTRTQRPSLYKVLLLNDDYTPMEFV
                     VHVLERFFNKNREEATRIMWHVHQKGVGVCGVFTFEVAETKVTQVTEFARQNQHPLQC
                     TMEKE"
     gene            complement(11389..12108)
                     /locus_tag="P5558_06450"
     CDS             complement(11389..12108)
                     /locus_tag="P5558_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY23983.1"
                     /translation="MNSNNRNSMFPFRRGGGKKRGKPGGGGNAGGRRQGGQGGGRRIP
                     NSAQELLGVLQPTTKSLAQVLAGNTRASGQIVHARNVLGQAERLVDERAIDRLPPAQR
                     EEFFEQVARLKLTLADAEREAEVEAETEVAEPVAPMSMDRLRALALSIATGGPAPGEP
                     ARDEPPSDPTTEATPAEPGAAEAAAAEPVGANADDDAEATPAEPADKPTRPKRANTLR
                     LKTLRHDPAEADELRRPEPAR"
     gene            complement(12760..13383)
                     /locus_tag="P5558_06455"
     CDS             complement(12760..13383)
                     /locus_tag="P5558_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRY23984.1"
                     /translation="MSGSTQPPATGPSSLSLAPEPRWFAAHELRTPLQAIKGGVELLL
                     EARGAGLRPELLDAVTLIATAAGDLERQLDLMAELAALAGGLRPEPRAVALHDLLARP
                     DVAQAIVPPPGLDHLPPTVRVLVAPEPFGRALTLLAGARQGPPGPLSCALHITGSADI
                     HLELDFIACGSGVGSVALRLSRELLGLSAVALDQPAKPGLVLAMRRC"
     gene            complement(13475..14260)
                     /locus_tag="P5558_06460"
     CDS             complement(13475..14260)
                     /locus_tag="P5558_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY23985.1"
                     /translation="MPTDRATRRLPPMAQVLLAGLLLAGCSGGNYTWGWYVVSPLNPQ
                     GQGNIAFLLSGMWQTISVSLLAITVSIPLGLLVALPGFGRNRVGQGFNRAYVEFIRSI
                     PILPLMLWVYYGLPVLTGINFDPFTAGVITLVISDSAFEAEIFRGGLQSIERGQHEAA
                     DSLGLSALDKLRFVILPQAIRRILPPLGNQFIYVLKMSSLLSVIGVFELTKKANELVV
                     TLYRPLEVYSFLILEYFLLVVVISLFVRRLERRMGADQRQRGR"
     gene            complement(14250..14990)
                     /locus_tag="P5558_06465"
     CDS             complement(14250..14990)
                     /locus_tag="P5558_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRY23986.1"
                     /translation="MAETIISLEGVTKRFGTFEALKKVDLTVALGERMVICGPSGSGK
                     STLIRCINRLEEHDEGRIVVDGRELDQDTRNVAAIRREVGMVFQQFNLFPHLTVLDNL
                     TLAPMRARGMSRKDAEEVARLYLDRVHIPEQADKYPAQLSGGQQQRVAIARSLCMRPR
                     IMLFDEPTSALDPEMISEVLDVMVELAREGMTMIVVTHEMGFAREVADTMVFMDGGEI
                     VEKATPAEFFVQPKSERTSLFLSRILAH"
     gene            15123..15917
                     /locus_tag="P5558_06470"
     CDS             15123..15917
                     /locus_tag="P5558_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY23987.1"
                     /translation="MPGQLGLRGVAALVLGILIVAGGASAQEQSRLPKILESGVLRVG
                     TTGDFNPMSFRDPGSNELKGYDIEAVEKLAADMGVTIEYVPTDWKTLVAGIVADKYDI
                     TTSASLSMDRAAVAGFSDPYVEFSTVPMTLAANVENYASWADLDTADVTIAVTLGTVF
                     EQQARSYFQNAQIKTVEAPAREWQEVISGRATASVTSNVDAAQLVTSYPELAVVKVEE
                     ARSRRPGAFLLPQDDQIWINYVNHWINLNHTSGWFAAQQQKWNLAN"
     gene            15936..18248
                     /locus_tag="P5558_06475"
     CDS             15936..18248
                     /locus_tag="P5558_06475"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HRY23988.1"
                     /translation="MRAVWRVDPAHRFVPQRPKTAGHLAGPAEPALPLGGIGTGAVSL
                     DRTGAFTRFTLKTGAVRLFHEPAAGFALRVAGPAGAPTTALQKRPADGRLAAWSFAEA
                     PAAAEFLFPWARLTWPAEATGGARAVLEASGPFVPGDLGASSLPLALFTWTLANPGPT
                     PLAVTLLAHWPNMNGWFSQFGRGRPHRCGVGNLNEVIDHPDLAAVRMDRVRTDDVIDE
                     EGVGEMVLALRRQPGLDVESLAAFDGGGDGAALWAAFAGAGRLPSGAPWLVEAPFDDR
                     DTAIPTAAIAAGCTLQPGEERVIEAVVVWDFPLVRFGLGRRHWRRYTAEWGRTGRKAL
                     AIAERALDAAPARAESVAGWQREVAASLGDAPEAGGLALQMLSFLVDGCTVATAPDAE
                     REEPAHFALIECPDYALYATMDLWIYASEAVLRTFPELEAQVLEDYARQLPRADARLR
                     LHALSGVPMPVKLAGMAPHDLGAPEEDPFHLASGYTWRDATGWKDLNAQLVIAIARAG
                     QVLGPGWQRRMLPTVGLALDALARFDRDGDGLIENDGVPDQTFDNIPMLGPSAYCGGL
                     WLAALLGGAAVADAADRPDLAAAWRATAAQGRQAFAAALWDGSRLRLDRDGPLKDALL
                     LGCVFGPFLARRYGFGDAVDPAMVRATLATLFAINFRKAGAGRAARLIVTAAGGPVAH
                     APGDVDASFQTSEALVGINLAFAAELQAFGLTSEAAEVRRALFAEVVERRGLLYRLPA
                     AIDLEAEVFRAPMNLRPLAAWLAQPWPFPG"
     gene            18260..18922
                     /locus_tag="P5558_06480"
     CDS             18260..18922
                     /locus_tag="P5558_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY23989.1"
                     /translation="MRLTFYTLPISNYAAKVELALRLKGLPYEALPPPDGYGSPAYKA
                     IVPAGTIPALVVTGLEAGELVLTESEVINEYLDEAWPDPPLLPGGPRARARTRQLARF
                     HDSRLEPVIRALFAQMAPAGRDAALVERQCALFATRLADLASMTTPQPWLAGPSLTLA
                     DLPYPPTLLMAGLMLEHLGRKLDLPPGLVAWHERALAHPSFAPVLEAQAAATRAWLAT
                     KV"
     gene            complement(18927..19724)
                     /gene="dapB"
                     /locus_tag="P5558_06485"
     CDS             complement(18927..19724)
                     /gene="dapB"
                     /locus_tag="P5558_06485"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRY23990.1"
                     /translation="MRIGVIGCAGRMGRANLAQLLGTEGVELAGGTERPGHEAIGTDL
                     GTLIGRDPVGILCTDDPSGLMTRAHAVIEFSTPAATTANAALAATHGCAHVIGTTALE
                     PADRAALAEAARRTAIVWAPNMSLGTNLLIGLAEKVAATLGPEFDIEIVEMHHRHKVD
                     APSGTALAIAEAAARGRGRPLDELVQRGRDGLTGPRPAGAIGLAALRGGDVVGDHTII
                     FAADGERLELSHKASSRAIYSAGAVRAALWAGRQPPGLYGMKDVLGL"
     gene            19815..20561
                     /locus_tag="P5558_06490"
     CDS             19815..20561
                     /locus_tag="P5558_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRY23991.1"
                     /translation="MSTSNLITALAEAMPLALRSYAVDAQPTGLVIVDEVNGFCTVGA
                     GNLAPPAPDAQVSAMVERTDTLARAFTAQRWPILAFLDTHEPGKPEPPYPPHCERGTG
                     EENLVPELAWLEGDAHALLIRKDCINGFVGGIEATFKGHGTSHNKVVDWVNVHRLEAV
                     LTTGICTDICVMDFVLTMLSARNHGLMPTLKDIVVLEPACATYDLPLGAARALGLPDT
                     AAHPKAETHHMGLYFMASRGAILASEVTGV"
     gene            21118..22125
                     /locus_tag="P5558_06495"
     CDS             21118..22125
                     /locus_tag="P5558_06495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY23992.1"
                     /translation="MGWSRRKILTTGGALAAAACIARPRCAAAAIDPAALVERAKALP
                     RLHTLIVGRGSEIVLAERFRGPAADRPVNIKSLAKTIIATLVGCAIDRGVLEGPDQRI
                     TTVLGPLVPADADPRLEEVTIGNLLSMQAGLERTSGRNYGAWVTSPDWVAHALSRPFV
                     AEPGGRMLYSTGSSHLLSAILTRATGESTLGLARAWLGEPLDIEIPPWDRDPQGIYLG
                     GNNLLLSPRAVFRFGRMINAGGTLDGRRIVAESWIATSWRPRTASRWTGHRYGYGWFI
                     TETGGHPHYYGWGYGGQMLHVVPSLNLVIAITSDPTVRSREAGHMAGLRALVAAVVGE
                     N"
BASE COUNT         3061 a         7662 c         7997 g         3522 t
ORIGIN      
        1 ctaagatgca ccgctcaccc atgctcgcaa gtgcgaaagg cgacgcaccg tggatttgtc
       61 ccgcttcgca caaggcgtcg catggacagc gcggcattcc cgttggctat cgtctgtgtt
      121 ccagatagct cttgccttga tctgaaaatc gtcgaggcgg ggaacgctag ccgggagggc
      181 ttcggcctcg ctggcggctg gcgttgcgat cagcgtcggc ctgccgcgcc gggcgcgtgg
      241 cgccgatccg gtcatggtgc ggctgacggt tgatgccaat atcggctacg cggaccttca
      301 tgtcgccgag gccgagggca tatcaccaag cacggcatcg acggctcggt gctgctcttc
      361 catgtcggct tcctcggcac gcaggccggc gcgcgagacg ggagctttct gcaagctctc
      421 cggcctgatc gagcgagcac tcttcgagtg gcgaccggcg gacgtcgagc cctatgtcct
      481 gcacttcctc gactgcttcg gcccggagcg gctgatgttc gcgagcaact ggccggtctc
      541 gaacctgcac ggcacctacg cgcgctggtg ggaggcgctg caggagatcc tcgatcggca
      601 ggggctcggc gtggacgagc gccggcactt gttcgagacg accgccgtcc gggcctatcg
      661 cctgccgcct gcccggcccg cctgaccggg cttctgccgt tcgcgtaccc tgccgctcag
      721 gcggggcggt cgcgcaggtc gacgaggccg gtcttgccgg tccagtcctc gccgcgcagc
      781 agatatatcg gccgctcggc gaacttagtc gccgcctggg gcgacgtcga gatggccagc
      841 tcacggtcga aatgcgaggt cgccggcatg tagcggatga cgaagcccgc ccggcggcgg
      901 gccgagcggt tgggctcgga gccgtgcacc agatagacgt cgtgaagcga gcaggcgccg
      961 gccggcaggg cgtccacctc gacccggtcg agatcgacgt cgcccggcag catctccgca
     1021 ttgagggcgg attcctcgct cggctggtcg gcatgcggga agagcgtccg ggtgcgttgc
     1081 gagcccggca cgtagagcat ggcgctgttc tcggcatcga cgtcgtcgag ggcgagccag
     1141 agcgtgcagg tggccaaggg ccggatcggc cagaaatgcc catcctggtg ccagggcacc
     1201 cggagcccgg tgccggccgg cttgcagaag atctggctgc cccagaggat cacgtccggg
     1261 ccgagcacct gctcgacgag gtcgaggaaa tccgcccggg tgcagaccgc gagccagcgg
     1321 tcggtgatgg cggccggcag gttgcccatg ccctcgatat gcgggcagac caggctctcg
     1381 gggcgctggc cgggcgtggc ggcgagcgtc tcctcgagga gaccccgcaa gccggcgagc
     1441 tcggcagcac cgagcccatg ggccggcttg accaggccgt ccaggcgata ggcctgcatc
     1501 tccgccgccg agaggcgggc gcggccgggg cggtggtcgt cgaaggctgc tgtcgtgctc
     1561 atgccggtgc tgtcctctcg tcgtccacgc atctgcgtgc ccgttgccgg cggggctcga
     1621 ccataggcga ggggcgtggc cggcggaagg cggtcggcac ctcgccgtca gaagctctcg
     1681 cgcaccttca ggtgcagatg ggcgcggggg cggcccgggc cgtgggcgtg ggtgtcgcgc
     1741 tgcggctcga cggggatcag cgagacattg ccgtgcatca cgccgcgctc gagaaagagg
     1801 ctctcggcat ggccgtgcag ctcccgggcg tcgccgcgca tgatggtgac ctcgatggcg
     1861 gtgtcgtggt cgagcggagc cgtggtcgtg gcgatggtgc ggtcgtgcag gtcgtgccgg
     1921 ctctcggcga ggcgccgggc gaggccgcgc atcgactggt ggcaggcata gctgacgacg
     1981 ccgacgcagt cgcctgtggc cttcccgtcg agggcgacct ggccgaggcc gcggcggacc
     2041 aggtcgcgga gcgcctcgga ccggctcgcc gcaccggact tctgcaggaa cgcgtcgagc
     2101 tcggcgagga gctcgtcatc gaacgacatc gtcacgcggc gcatgaccct ctccccgtcc
     2161 ccatgcccgg ccgtctcggc ctgagccatc cttgccgaaa aggccctcca caggaagggg
     2221 cggcagccgg gggtcaaccc tgctcggcgg cggccgcgcc cagaagctcg cgcagcaggg
     2281 gattgggaaa gcggcggcgc agcgtgacgg cgaagaaggt ctcgctcagt cgcggcaact
     2341 gcacagcctc cctgagccgg ccgctggcca gctcgtcccg gacgacgatc ggcgggacca
     2401 cggcgaggcc gatatcctcg cgcgccagca gacgcagcag cgccatgtcg tcgacctccg
     2461 cggcgatgcg cggacggatg ccgagccggt cggccagcgc gtcgaagccg gtgcggatgc
     2521 cactttgcgc cgtcggcagg accagtggct cgcgctgcag cagcacttcg agggagcggt
     2581 cttcgcccag gcgtcgcggc gtgccgatca ggctcaccgg ctgctcggcg atcggctgcg
     2641 cccgccaggg ggtcgcggcg tcgccgggcg gggtggcatt cgccagcacg acatcgaggc
     2701 ggtgcgcctc gaggctcgcc aggagatccc cgaggccgcc cgagcgcagc acgatctcga
     2761 catcgtcgcg cccgagcagc ggccgcagga aggcgatctg aaagttgcgc gatagcgtcg
     2821 ccaaggcgcc gacccgcagc acgcgtcgtc cccggccgct ttcggccagc gtgtccagga
     2881 gctcgtcgcc ggcagcgaag atggcgtccg cgtgatcgag ggcgatccgg ccggcctcgg
     2941 tcggcacgag ctgcctgcca cggcgctcga acagggcatg gccgagctcg tcctcgagct
     3001 tgcggatctg gatcgagacg gcggattgcg agaggttcag gcgctcggcc gcccgcgtga
     3061 gattgccctc gtgcgcgacc gcccagaaat agcgcagatg gtggaagttc agcgtcgcca
     3121 tgggtccgtt ctactgagga gaacaaagcg tttcaaacaa tgaatttttt ttgtcgatga
     3181 tggtcggcta gaccctcccc cgatcacctc ccctcgctgc cgccccttcg agctggtcgg
     3241 agaattcgat gccgtcggtc ctcgccctgg tactctttct ggcccctttg agcctcgttg
     3301 ccgtggcgat gctcgcccga cgcgagcccg gctcgcggcc gcgccgggtg ctccgcgcgg
     3361 cggagctggc gacgctcctg acgctgggca tggccgttct ggcgggactc gccgtcgctc
     3421 tcgccgggcc cgggacgagc ccggttctgg ggttgggcgg gattggcctc gcgatccgct
     3481 tggacgcgct gagcgttctg atcttcggcc tcgtcgcctt cgtcggtgcc gtcgtcgtcc
     3541 ggttcagtcg caattatctc gacggcgacg gccgccaggg cgccttcatc ggcgggctct
     3601 gcctgaccct ggccgcggta tcgctcctcg tcctcgccgg caatctcggc cagctcgtgc
     3661 tgggctgggt gctcaccagc ctcgctctgc atcgcctcct ggtcttctac ccggatcgtc
     3721 ggggggcggt gctggccgcc cggaagaaat tcgtggcagc acggatcggc gatgcctgtc
     3781 tcgttgccgc ggtcgtgctg ctgctgcggg cgttcggcac agccgatatc ggcaccatgc
     3841 tcgaggcggc acgcctcggc ggcgcgacgc tgccgccgac aggcgtggcg accgccgccg
     3901 tgctcctggc gttcgcggcg ctgctcaagt cggcgcagtt tccgacccat ggctggctga
     3961 tcgaggtgat ggagacaccg acgccggtct cggccctgct gcatgccggc atcgtcaatg
     4021 ccggcgggtt tctggtcatc cgcttcgccg atgtcatgct cctggcaccg ggggcgctgc
     4081 atctcctggc cgtggtcggc tgctgcacgg cgctcgtcgg cgccaccgtc atgctgacgc
     4141 agaccagcat caagctgtcg ctggcctggt cgacggtggc ccagatgggc ttcatgatgc
     4201 tgcaatgcgg gctcggcgcg ttcccgctcg ccgtcctgca catcgtcgcc cactcgctct
     4261 acaaggcgca tgcctttctc gcctcgggca gcgtgatcgg cgccgcaccg gctccgacag
     4321 ccgaagggcc gcgaccgtcc cgggcgctgg cgagcctcgg cacggcgctc gggctctatc
     4381 tcggccttgc ctggcttttc gggatcaccc cggctgcgga gccggtccgg ctggcgctcg
     4441 gtgccatcct ggcgatgggc ctgagcgtgc tcgtcatgcc ggcggtgacg ggtccgttcg
     4501 acgcccggct cctcggccgc acgctcgggg cggcggccgg tgtcacggtt gcctatctgg
     4561 cggtgcaggc cggcgcccat ctgctgctcg cgtcgaccct gccgccgcga ccggggccga
     4621 gcacggccac gcacgtgatc atcggcctcg ccctggtcgc cttcgccgcg gtgacagcta
     4681 tgcaggcgat cgccccgtcc ttggctcttc gcccgggctg gcaggccctg cgcgtccacc
     4741 tcgccaatgg cctctacgtc aatgccgccg tcaatcgcct ggccggtgcc ctccggcgcc
     4801 ccgccacggt cgccccccct tcgatcctcc aagggaacca ctcgtcatga tcagccaacc
     4861 tccgatcgag gacccgagca gggcggagat cctcgccgcc gcccttcggg cgacccgccg
     4921 gatagcgccg ctctggccgc tgcagggctt cgtcgcggtg aatccgtttc tcggtcttgc
     4981 cgatcaccgc ttcgccgagg ccgcgcgggc gatggctgcg tccgcgggtg cgcggttgac
     5041 gatgccgcgg gcgttctatg cgaaggcgat ccggggcggg cggatcacgg cccttgatct
     5101 cgccgatgcc ctggccgagg ccgaggacgg cggcgacctg ccggcggatg gcgaagctct
     5161 gcgcgccgcc gccctggcac cggacccgga cgtggcgccg aggcccttgc cgacggtcgc
     5221 cgatgtcgcg ggtgccgcga cgggccgtga ctgggcgggc ttcgtcaccg atcggctgtc
     5281 cgcctgggcc gccgccttct tcgatgcggg gcaggcggcc tggcgctcgc cctggcggga
     5341 tctcggtccc tacgccgcat ggcgggccga ggcgctggtc gaccgcacgc cggaaaccat
     5401 ggggctcgcc ggcttccggc gggcggtcga gggcctgccg ggcgatgcca gcgacgccct
     5461 cgaggtggcc acgcggcgga tcggcatgcc ggcagcggcg ctcgaggctt acggccatcg
     5521 gctcctgatg acgatcggtg gctggtccgg gcacgcccgg cagcgggtct gggagagcga
     5581 gctgcacggc cgtccggacg cgagcctgct cgagcttctg gcgatccggc tcgcctggga
     5641 ggcagcgctg ctcgattgcc tcgcggacgc gccggccgtc gagatggcct ggcatgccgc
     5701 gctcggcgcc tacgcgacct cgccggcgag ccatccggcg cttgctgtcg attgcctcct
     5761 gcagctcgcc tacgagcggg cgtggcagcg ggagctggca gcccgcttcc gggcccgccc
     5821 cgaggccgcg ccggccgagc ggccggcggt gcaggcggtc ttctgcatcg acgtgcgctc
     5881 ggaggtgttc cgccgggcgc tcgagcgggc ggcaccggag gtcgagacga tcggcttcgc
     5941 cggcttcttc ggcctcgcca tcgactacgt gccgttcggc gagatggccg gccgggcgca
     6001 gtgccccgtc ctgctgcggc ccggtgtcac cattcgcgag acagtgcgcg acgccggccc
     6061 ggccgaagct ctggggctgc tcgcctgcca tcggttgcgg cgccgcgccg ccggcgcctg
     6121 gagcgctttt cgccaggcgg ccgtctcgtc cttcgccttc gtcgaggcga tggggctcac
     6181 ttttctcggc aggctcgccc gggacgggct cgggccggcc ttgcctgcct cggtcgcggc
     6241 cggcggcggc gatccggtgc tcgccggacg cctcgagccg agcctcgagc ccggctcgct
     6301 cgccgggcgg gccacgggca tcgacatcga gggcagggtc aggatggccg ccgcggtgct
     6361 cgaggccatg gggttgcgtg acggcctggc gcggctcgtg ctgctcgccg gtcacggctc
     6421 gaccaccacc aacaaccctt atggcagcgg tctcgattgc ggcgcctgcg gcggtcatgc
     6481 gggcgaggcc aatgcgcggg tcgcggcgag gctgctcaac gacccctcgg tccgtgccgg
     6541 cctcgccctg cggggcatcg cggtccccgc cgacacggtc ttcgtcggcg gcctgcacga
     6601 caccaccacg gatgcgcttc gcatcctcga cccgcgcgat gtcccggcga gccacgcggc
     6661 ggacctcgcc cggctggtcg cctggctcgc ggcggccggc gagctggcgc gcggcgagcg
     6721 ggccccggcc ttgcggttga gcacggatcg gccgatcgat gcgcagatgc gcgcccgcag
     6781 ccgcgactgg agcgaggtgc ggccggagtg gggcctcgcc ggctgtgccg ccttcgtggc
     6841 ggcaccccgc caccgcacgc gggatatcga cctcggcggc cgggccttcc tgcattccta
     6901 cgactggcgc cgggacgagg gctttcgcac gctcgagctg gtgatgacgg cgcccatggt
     6961 ggtcgcgagc tggatcagcc tgcaatatta cggctcgacc gtcgacaacc gcgtcttcgg
     7021 cgccggcaac aaggtgctgc acaatgtcgt cggcaggctc ggcgtgatcg agggcaatgg
     7081 cggcgacctg cgcgccgggc tgccctggca ggcggtgcat gacggcgagc ggctggtgca
     7141 cgagccgctc cgcctcacgg tggtgatcga ggcgccgatc acggctatca acgaggtgat
     7201 cgcccggcac gggacggtgc ggcaactcgc cgaccatggc tggctgcacc tcctggccct
     7261 cgatgacgac ggcctgatcg gccagcgcta tgtgggcggg ctggagtggg caccgatgcg
     7321 cgcggccatg gcggaagcgg cctgagcgtc gcggcccggg cgctcaggcg cgggtctggg
     7381 agcggttcgc ggggcgatcc gccggctcgg cgccggcgag atcgtcgatg atcgggcagt
     7441 cggggcgctg gtcgccgtgg caatgatcgg ccagatggcg gagcgtgcgg cgcatcgcct
     7501 cgagctcggc gatcttctcc tcgagctccg ccacgtgctc gagggcaatt cgcttgacct
     7561 cggcgctggc ccggttggtg tcccgccaga gcgcctcgag ccggccgatc tgcgccacgg
     7621 agaagcccag gcggcgggcc cggcggatga aggcgagccg gtggacgtcg ttcgggccgt
     7681 agacgcgata gccgctctcg ctgcgggccg ccggtggcag aagcccgatg ctctcgtagt
     7741 agcggatcat cttggcggag accccggagg cgctggcgac ctcaccgatg ttcatgggcc
     7801 atgctcaggg aaacgagccg gcgcggccga ggacggcctc gatgccgtgg agcggcttgc
     7861 ccatgaagcg gctctgcggg tccggccggt agtcggcgaa ggggccgata tccatgtagc
     7921 cggcccgcct gtagagggcg agcgcctcgg gctgggcgat gccggtttcc agcctggagg
     7981 tccggcatcc cgaagccatc gccagggatt cgaggtggtc gagcaaccgc cggccgacgc
     8041 ctctgccgcg ggctgccggc gccacataca tgcgtttgat ctcggcgtcg tcggcttcga
     8101 gcacgaccgc accgcagccg accggcgcgt cgcccagccg ggccacgacg aagcgcaccg
     8161 ctggttgtcg cagcgcgtcc atcgtcaggc catgattgct ggccggcggg tagagggtcg
     8221 cgaggtaggc gtcgagggcc ccgagcaggg accgcaccgc cggctccgcc aaatcgccgg
     8281 gctcgatccg catgccgggg tcgtgccggc ccgcgccggc caggctcacc cgacggtctc
     8341 gggcagctcg gcgtccggcg tgtccaggcc cgacggactt gcctcgggcg attcgtcctc
     8401 gtcgttcagg acggcgagcg gcgcgccctt cgtcgtggcc aggacctcga aggacggctt
     8461 gtcgtcgacg atggtgacga gcaccttgcc gcccttggcg aggcggccga acagcagctc
     8521 gtcggcgagc ggcttcttca aatgctcctc gatcacccgg gcgagcggcc gcgcaccata
     8581 gagcggatcg tagccgcgct cggcgagcca ggacttggcc ggctcgtcga gctcgatcgc
     8641 cacccgcttg tcggcgagct gggcggcgag ctgctggacg aacttgtcga cgaccgagcg
     8701 gatgatcgag ggcgtgaggc cggcgaacgg gatggtggcg tcgagccggt tgcggaactc
     8761 gggcgtgaac agccgcttga tcgcctcggt gtcctcgccc tcgcgggcga tgccgccgaa
     8821 gcccatcacc ggccggctca tgtcggaggc gccggcattg gtcgtcatga tcaggatgac
     8881 attgcggaaa tccaccgtct tgccgttgtt gtcggtgagc ttgccgtaat ccatgacctg
     8941 caggaggatg ttgaagacgt cggggtgcgc cttctcgatc tcgtcgagca gcaggacggc
     9001 atgcgggtgc tggtcgatgg cgtcggtcaa aaggccgccc tggtcgaagc cgacatagcc
     9061 gggcggcgca ccgatcagcc gcgatacggc atgacgctcc atgtattccg acatgtcgaa
     9121 gcggacgatc tcgatcccca tgatggcggc gagctggcgc gcgacctcgg tcttgccgac
     9181 gccggtgggg ccggagaaga ggtagcagcc gatcggcttt tgcgggtcgc gaaggccggc
     9241 acgactgagc ttgatcgccg aggcgagcgc ctggatggcc cggccctggc cgaaaaccac
     9301 gccctcgaga tcgctctcga gcgtcctgag cgcatccttg tcccgggcat tcaccgcctt
     9361 gatcggcacg cgcgcgatct tggccacgat ctcctcgatg tccttgggcc ccacggtctt
     9421 gcgccgctgg ctctcgggct tgagcatctg ggcggcgccg acctcgtcga tcacgtcgat
     9481 cgccttgtcg ggcagcttgc ggtcgtggat gtagcggttg gcgagcttga ccgcggcctt
     9541 cagcgcctcg ttcgagtact tgatccggtg atgctcctcg aagctcggcc ggagcccctg
     9601 caggatcttc accgcctcgt cctcggaagg ctcggggacg tcgatcttct ggaaccggcg
     9661 caccagcgct cggtccttct cgaaatagtt gcggaattcc ttgtaggtcg tcgagccgat
     9721 gcagcgcagc gcaccgccgg ccagggcagg cttgaggatg ttcgaggcat cgagcgagcc
     9781 gccgctggtg gcaccggcgc cgatcacggt atggatctcg tcgatgaaca ggaccgcgcg
     9841 ctcgttggcc tcgagctcgc cgatcaccgc cttcagacgc tcctcgaaat cgccgcgata
     9901 gcgcgtgccg gcgaggagcg cacccatgtc gagggcgaag atgatggcat tcttgaggac
     9961 gtccggcacc tcgccgcgga tgatccgcag cgccaggccc tcggcgatcg cggtcttgcc
    10021 gacgccggga tcgccgacga agagcgggtt gttcttggag cgtcgacaga ggatctggat
    10081 ggtccgctcg atctcgtgct gccggccgac caggatgtcg atcttgccgg ccgccgcctt
    10141 gtcgttgagg ttgacgcaat aggtcgacag cgcctcgctg gcggcctcgc gcttctcctc
    10201 gccctggctc gcttcctccg cacccttcac gcgacgctcc tcgctgaggc ttggcttctt
    10261 ggcgatacca tgcgcgatat aattcaccgc atccagccgc gacatgttct gctcctgcag
    10321 aaaccacacg gcatgggatt cgcgttcgga gaagagggcg accaggacgt tcgcacccgt
    10381 cacctcgctg cgtcccgagg actgcacgtg gatggcggcg cgctggatga cgcgctggaa
    10441 gccggcggtc gccttggcat cgacgacgcc gtcgacgacg aggccggaga gctcgtgatc
    10501 gaggaagtcg gtgaggccga ggcgcagctt gtcgatctcg acggcgcagg ccttcatgac
    10561 cgccaccgcg tcctgatcct cggtcagcgc catcaggagg tgctcgagcg tcgcgtattc
    10621 gtgctgtcgc gcattggcca gcgccagcgc ccggcggagg gtcttctcga gagtgcgcga
    10681 gagcatcgtc ttctcctagc cgacccggag gcgacttcgg ccggccgcac cgaccgtcgg
    10741 caggccaaca tccttgatga tacgcgcgac ggcgggccga gtgccagccg gaaagcgcga
    10801 gcggctcgag ccgggccgca gcgatgcggc cggttgcgac cgcccgcttg ccggaccggt
    10861 cattccttct ccatcgtgca ctgcaacgga tgctggttct gccgggcgaa ctcggtgacc
    10921 tgggtgacct tggtctcggc cacctcgaag gtgaagacgc cgcagacccc cacacccttc
    10981 tggtggacgt gccacatgat ccgcgtcgct tcctcgcggt tcttgttgaa gaaccgctcg
    11041 agcacgtgca cgacgaactc catcggcgtg tagtcgtcgt tcagcagcag gaccttgtag
    11101 agcgacgggc gttgggtgcg ggtgcgcgtc ttcgtcgcga cgtcgccctt gccgatccgc
    11161 tcctcgttgt tgccgtcgct catcgtcccc catcggcttc gttgccggcc gaccgtggcc
    11221 gacgccatcc gtcggaaaga tagttcaatc ggcggtgcgc acaaatgcct ttccgcgtcg
    11281 gctcggtgct gacgcccata ttggcgcata cccgacaaga ttcagttaag gcgcctgcac
    11341 ggcggccgcg acggcccttc gccctgaatc aacggtgctg cccggcgatc agcgggccgg
    11401 ctctggccgg cgcagctcgt ccgcttcggc gggatcgtgg cgcagggtct tgagccgcag
    11461 cgtattggcg cgcttgggcc gcgtcggctt gtcggcgggc tcggccgggg tcgcctcggc
    11521 gtcgtcgtcg gcattggccc cgacgggttc ggcagcagcg gcttcggcag cgccgggctc
    11581 ggccggggtc gcctcggtcg tcgggtcgga cggtggttcg tcccgagccg gctcgcccgg
    11641 ggccgggccg cctgtcgcga tgctgagggc gagcgcccgc agccggtcca tggacatcgg
    11701 cgccactggc tccgcgacct cggtctcggc ttcgacctcc gcttcgcgct cggcgtcggc
    11761 cagcgtcagc ttgagtcggg cgacctgctc gaagaactcc tcgcgctgcg ccggtggcag
    11821 gcgatcgatg gcccgctcgt cgaccagccg ctcggcctgg ccgaggacgt tgcgtgcatg
    11881 cacgatctgg cccgaggcac gggtgttgcc ggccagcacc tgggccagcg acttggtcgt
    11941 gggctgcagg acaccgagca gctcctgggc ggaattggga atccgccgac cgccgccctg
    12001 acctccctgg cgccgtccgc cggcattgcc gccgccgcct ggcttgccgc gcttcttccc
    12061 gccgccgcga cggaacggga acatcgagtt ccgattgttg ctgttcaccc gtatcctttc
    12121 cagagcttgc cgatcgtctc ttggctatcg tccacgcaca gatcgcgcgg ctgccgtgtc
    12181 agcggccgag catcggcaaa tgccgccccg accatccgca ggtcgccgca gcctcacctg
    12241 ccagaggcgc tgcccggcta acctgctttc cggctcgccc atgcgtcatg gaacattcgt
    12301 tcccgattgg cccacccgat ccggtcgccg ccaccagcgg ccgactcacg ccggctgtgc
    12361 cctcgacaac gccggaacgt accgcttttg ccaaaccgat cggcgcacgg gcttgcttgt
    12421 ccgacccatg tgagcccaag ccgatcgtgg atctcgatcg ccgtgccgca tcgagccgat
    12481 ccgatatcgc ttcagtccag tcccgacgcc cggcagccac ttccgggtcc ggatacggtc
    12541 gaccgcccct gcagtctccc gcttctgtgc cgaatgtgcg cggggatcga atgcttggtt
    12601 catatgcccc ttcgaccttt accctgcaag accggaaaat caagtgaaag ctaggtgatg
    12661 caccctagcg gcagccggta ggtcggttcg cgttgcgttc gaggcgcagc ccgggccact
    12721 caaccgaccg aggcaggctg gtcgcgccgc caggcgacct cagcagcgac gcatcgcgag
    12781 caccagtccg ggcttcgcag gctgatcgag tgcaacggcc gacagtccga gcagttcccg
    12841 ggagagccgc agcgccaccg agccgacgcc gctgccgcag gcgatgaagt cgagctcgag
    12901 gtggatatcg gcggacccgg tgatatggag cgcgcaggac aggggtcctg gcgggccctg
    12961 ccgggcgccg gccagcagcg tcagggcgcg gccaaagggt tctggtgcca ccagcacccg
    13021 cacggtcggg ggcaaatggt cgagccccgg gggcggcacg atcgcctgtg cgacgtccgg
    13081 ccgggcgaga agatcgtgca gggccaccgc gcgtggctcc gggcggaggc cgccggcgag
    13141 ggcggcgagc tcggccatga ggtcgagctg gcgctcgaga tcgccggcgg cggtggcgat
    13201 cagggtcacg gcatcgagca gctccggccg caacccggcg ccccgcgcct cgagcaggag
    13261 ctcgaccccg cccttgatcg cctgcagggg cgttctgagc tcgtgcgccg cgaaccagcg
    13321 gggctcgggc gccagcgaga ggctcgacgg cccggtggca ggaggttgcg tggagccgga
    13381 caaggcgaaa tccttctagg acggcgtcgc tgccggccga ggaaagcgag cctagccgga
    13441 caggcttaag atatcgttgc gcgcagccgg atgatcagcg tccgcgctgg cgctggtcgg
    13501 cacccatgcg tcgctcgagc cggcgcacga agagcgagat gaccaccacg aggaggaagt
    13561 attcgaggat gaggaagctg tagacctcga gcggccggta gagggtcacc accagctcgt
    13621 tggccttctt ggtcagctcg aagaccccga tcacggagag cagcgaggac atcttcagga
    13681 cgtagatgaa ctggttgccg agcggcggca ggatgcggcg gatcgcctgg ggcaggatga
    13741 cgaagcgcag cttgtcgaga gcggagaggc cgagcgaatc cgcggcctcg tgctggccgc
    13801 gctcgatcga ctgcagcccg cccctgaaga tctccgcctc gaaggcgctg tcggagatga
    13861 ccagcgtgat cacgccggcg gtgaacgggt cgaagttgat cccggtgagc accggcaggc
    13921 cgtaatacac ccagagcatc aggggcagga tcgggatcga gcggatgaac tcgacatagg
    13981 cgcggttgaa gccctgaccg acccggttgc ggccgaagcc cggcagggcg acgaggaggc
    14041 cgagcgggat cgagacggtg atggcgagca aggagacgct gatcgtttgc cacatgccgc
    14101 tcaagaggaa ggcgatgttg ccctggccct gggggttgag cggcgagacg acataccagc
    14161 cccacgtgta gttgccgccg ctgcagccgg cgagcaggag gccggccagc agcacctgcg
    14221 ccatcggcgg gaggcgccgg gtggcccggt cagtgggcaa ggatgcggct caggaagagg
    14281 ctggtgcgct cgctcttcgg ctggacgaag aactcggccg gcgtcgcctt ctccacgatc
    14341 tcgccgccgt ccatgaagac catggtgtcg gccacctcgc gggcgaagcc catctcgtgg
    14401 gtgacgacga tcattgtcat gccttcccgg gcgagctcga ccatgacgtc gagcacctcg
    14461 ctgatcatct cggggtcgag cgccgaggtc ggctcgtcga acagcatgat ccggggacgc
    14521 atgcatagcg agcgggcgat ggcgacgcgc tgctgctgcc cgcccgaaag ctgggccggg
    14581 tacttgtcgg cctgctcggg gatgtgcacg cggtcgagat agagccgcgc cacctcctcc
    14641 gcgtccttgc ggctcatgcc gcgggcgcgc atcggggcga gggtcaggtt gtcgagcacc
    14701 gtgaggtgcg ggaagaggtt gaactgctgg aacaccatgc cgacctcgcg gcggatggcg
    14761 gcgacgttgc gcgtgtcctg gtcgagctcg cggccgtcga cgacgatgcg gccctcgtcg
    14821 tgctcctcga gccggttgat gcagcggatg agggtggact tgccggagcc ggacgggccg
    14881 cagatcacca tgcgctcgcc gagcgccacg gtcagatcga ccttcttcag cgcctcgaac
    14941 gtgccgaagc gcttggtcac gccctcgagg ctgatgatcg tctcggccat gtcctgcccg
    15001 tttgctgccg tgccgcaaag ctagcggtct gcggtcgcac ggcaagcccc ggtggacaag
    15061 ggccgtggcc cgctgcatat ccgggggcca ggggagagaa cccacctcca gggagaaacg
    15121 caatgcccgg tcagctcggt ctgcgcggcg tcgccgcgct cgttctcggc atcttgatcg
    15181 tggccggcgg ggcctccgcg caggagcagt cgcgcctgcc gaagatcctc gagagtggcg
    15241 tgctcagggt cggcacgacc ggcgatttca acccgatgag ctttcgcgac ccgggctcga
    15301 acgagctcaa gggctatgac atcgaggcgg tcgagaagct cgccgccgac atgggcgtga
    15361 cgatcgagta cgtgccgacc gactggaaga cgctggtcgc cggcatcgtc gccgacaagt
    15421 acgacatcac gacgagcgca tcgctcagca tggaccgggc cgccgtcgcc gggttcagcg
    15481 atccctacgt cgagttctcg accgtgccga tgacgctggc cgccaatgtc gagaactatg
    15541 cgagctgggc cgatctggac acggccgacg tgaccatcgc tgtgaccctc ggcacggtct
    15601 tcgagcagca ggcccgcagc tacttccaga acgcccagat caagacggtc gaggcgccgg
    15661 cgcgcgagtg gcaggaggtg atctccgggc gggcgacggc cagcgtcacc agcaatgtcg
    15721 acgcggcgca gctcgtcacc agctacccgg agctggctgt ggtgaaggtc gaggaggcgc
    15781 gctcgcgccg gcccggcgcc ttcctcctgc cgcaggacga ccagatctgg atcaactacg
    15841 tcaatcactg gatcaacctg aatcacacga gcggctggtt cgcggcccag cagcagaagt
    15901 ggaacctcgc caactgagcg actgaaccga cgctcatgag ggccgtctgg cgggtcgatc
    15961 cggcgcaccg cttcgtgccg cagcggccga agacggcggg ccatctggca ggccccgccg
    16021 agccggccct gcccttgggc ggcatcggca ccggggcggt cagcctcgat cgcaccgggg
    16081 ccttcacccg cttcactctg aagaccggtg ccgtccgcct cttccacgag ccggccgccg
    16141 gcttcgcctt gcgggtggcg gggcctgcag gcgcgcctac gactgcgctg cagaagcggc
    16201 cggcggacgg caggctcgcc gcctggtcgt tcgccgaggc gccggccgcg gccgagtttc
    16261 tcttcccctg ggcccggctg acctggccgg cggaagcgac cggtggtgcg cgggcggtgc
    16321 tcgaggcaag cggtcccttc gtgccgggcg atctcggggc gagcagcctg ccgctggcgc
    16381 tcttcacctg gaccctggcc aatccggggc cgacgccgct ggcggtgacg ctgctcgcgc
    16441 actggccgaa catgaacggc tggttcagcc agttcggccg cggccggccg caccgctgcg
    16501 gggttggcaa cctcaacgag gtgatcgacc atcccgatct ggcagccgtg cggatggacc
    16561 gggtacggac tgatgacgtc atcgacgagg agggtgtcgg tgagatggtg ctggcgctcc
    16621 gccggcagcc gggcctcgac gtggagagcc tcgccgcctt cgatggcggc ggcgacgggg
    16681 cggcactctg ggcagcgttc gcgggcgcgg gccgcctgcc gtcgggcgcg ccctggctgg
    16741 tcgaggcgcc gttcgacgat cgcgacacgg cgatcccgac cgccgccatc gccgccggct
    16801 gcacgcttca gccgggcgag gagcgggtga tcgaggccgt ggtcgtctgg gatttcccgc
    16861 ttgtccgctt cggcctcggc cggcggcatt ggcggcgcta cacggccgaa tggggccgga
    16921 ccgggcgcaa ggccctggcg atcgccgaac gggcgctcga tgcggcaccg gcccgggccg
    16981 agagcgtggc cggctggcag cgggaagtgg cggcgagcct gggcgatgcg cccgaggccg
    17041 gggggctcgc cttgcagatg ctgtccttcc tggtcgacgg ctgcacggtc gcgacggcgc
    17101 ccgatgcgga gcgggaggag cctgcgcatt tcgccctgat cgaatgtccg gactatgccc
    17161 tctatgcgac catggacttg tggatctacg cctccgaggc ggtgctccgg acgtttcccg
    17221 agctcgaggc gcaggtgctc gaggactatg cgcgccagct accgcgcgcc gatgcccggc
    17281 tgcggcttca tgcgctctcg ggcgtgccca tgccggtcaa gctcgccggc atggcgccgc
    17341 acgacctggg ggcgcccgag gaggacccgt tccacctcgc cagcggctac acgtggcggg
    17401 acgccaccgg ctggaaggac ctgaacgcgc agctggtgat cgccatcgcc cgggccgggc
    17461 aggtgctggg gccgggctgg cagcggcgca tgctgccgac ggtcgggctg gcgctcgacg
    17521 cgctcgcccg cttcgatcgc gacggcgacg ggctgatcga gaacgacggc gtgccggacc
    17581 agaccttcga caacatcccc atgctgggcc cgtcggccta ttgcggcggg ctctggctgg
    17641 cggcgctcct gggcggggcg gcggtcgccg atgcggccga ccggccggac ctcgcggcgg
    17701 cgtggcgggc gacggcagcg cagggccggc aggctttcgc ggcggcgctc tgggacggca
    17761 gccggctcag gctggatcgc gacggcccgc tgaaggacgc cctcctgctg ggctgcgtct
    17821 tcggcccctt cctcgcccgg cgctacggct tcggcgacgc ggtcgatcct gccatggtcc
    17881 gggccacgct ggcgacgctg ttcgcgatca atttccggaa ggccggggcc ggtcgcgccg
    17941 cccggctaat tgtgacggcg gccggcgggc cggtggcgca tgcgccgggc gatgtcgacg
    18001 cctccttcca gacgagcgag gccctggtcg gcatcaacct ggcgttcgcc gccgagctgc
    18061 aggccttcgg cctgacttcc gaggcggcgg aagtgcggcg ggcgctcttc gccgaggtgg
    18121 tcgagcggcg cggcctgctc tatcgcctgc cggcggcgat cgacctcgag gccgaggtct
    18181 tccgggcacc gatgaacctt cgcccgctcg ccgcctggct cgcccagccc tggccatttc
    18241 ccggataggc gccgaagcca tgcgactgac cttctacacc ctgccgatca gcaactacgc
    18301 cgccaaggtc gagctggcgc tccgcctcaa gggcctgccc tacgaggcgc tgccgccccc
    18361 cgacggctac ggctcgccag cctacaaggc gatcgtgccg gccggcacga taccggccct
    18421 cgtcgtcacc gggctcgagg cgggcgagct ggtgctgacc gagagcgagg tgatcaacga
    18481 gtacctggac gaggcctggc cggacccgcc gctcctgcct ggtggtcctc gggccagggc
    18541 cagaacacgc cagctcgccc ggtttcacga cagccggctc gagccggtga tccgcgccct
    18601 ctttgcgcag atggcgccgg ccggccgcga cgcggcgctg gtcgagcgcc agtgcgcgct
    18661 cttcgcgaca cggctcgccg acctcgcttc gatgaccacg ccgcaaccct ggctcgccgg
    18721 gccgagcctg accctggccg acctgcccta tccgccgacc ttgctcatgg ccgggctgat
    18781 gctggagcat ctggggcgaa agctcgacct gccgcccggg ctcgtcgcct ggcacgagcg
    18841 ggccctggcc catcccagct tcgcgccggt gctggaggcc caggcggcgg cgaccagggc
    18901 gtggctggcg accaaggtct gaggcgtcag agcccgagca cgtccttcat gccgtagagg
    18961 ccgggcggct gccggccggc ccagagtgct gcgcggacgg cgccggcgct gtagatggcg
    19021 cggctcgacg ccttgtggga gagctcgagg cgctcgccat cggcggcgaa gatgatggtg
    19081 tggtcgccga cgacgtcgcc accgcgcagg gccgcgaggc cgatcgcacc cgccgggcgc
    19141 gggccggtga ggccgtcgcg gccgcgctgg accagctcgt cgagcggccg gccgcggccc
    19201 ctggcggcgg cctcggcgat ggccagcgcc gtgccggagg gggcatcgac cttgtggcgg
    19261 tggtgcatct cgacgatctc gatgtcgaac tccggcccga gcgtcgccgc caccttctcg
    19321 gcgaggccga tcaggagatt ggtgccgagg ctcatgttgg gcgcccagac gatggcggtg
    19381 cgcctggcgg cttcggccag cgccgcccgg tcggccggct caagggcggt cgtgccaatc
    19441 acgtgggcgc agccatgggt ggcggcgagg gcggcattgg cggtggtggc ggccggcgtc
    19501 gagaactcga tcaccgcatg ggcacgggtc atgagcccgg acgggtcgtc ggtgcagagg
    19561 atgccgaccg ggtcgcggcc gatgagcgtg ccgaggtcgg tgccgatcgc ctcgtgaccc
    19621 ggccgctcgg tgccgccggc gagctcgacg ccctcggtgc ccagaagctg ggccaggttg
    19681 gcgcggccca tccgcccggc acagccgatc acgccgattc gcaagggctc tctcccgtca
    19741 gacgttgcat gcgcgtcgct tgccctgttt agtccaccgt cgagtcgagc cggtcaagtg
    19801 gaggagccct gcccatgtcg acatccaatc tgataacggc cttggccgaa gccatgccgc
    19861 tggcgctgcg gtcctacgcc gtcgacgccc agccgaccgg gctcgtcatc gtcgacgagg
    19921 tcaacggctt ctgcacggtg ggcgccggca acctggcgcc gccggcgccg gatgcgcagg
    19981 tcagcgccat ggtcgagcgc accgacacgc tcgcccgggc cttcaccgcc cagcgctggc
    20041 cgatcctcgc cttcctcgac acccacgagc cgggcaagcc cgagccgccc tacccgccgc
    20101 attgcgagcg cggcacgggc gaggagaacc tcgtgccgga gctcgcctgg ctcgagggcg
    20161 acgcccatgc gctcctgatc cgcaaggact gcatcaacgg cttcgtcggc ggcatcgagg
    20221 cgaccttcaa ggggcacggc accagccaca acaaggtggt cgactgggtc aacgtgcacc
    20281 ggctggaagc ggtgctgacc accggcatct gcaccgacat ctgcgtcatg gacttcgtgc
    20341 tcaccatgct ctcggcccgc aatcacggcc tgatgccgac gctcaaggac atcgtcgtgc
    20401 tggagcccgc ctgcgccacc tacgacctgc cgctgggggc ggcgcgtgcg ctcggcctgc
    20461 ccgacacggc ggcccacccc aaggcggaga cccaccacat ggggctctat ttcatggctt
    20521 cacgcggtgc catcctggcg agcgaggtga cgggcgtctg agcgatgaag gaataatagc
    20581 ctatttcggg caggccgtca agatctaccc cggttcgttc ggcaaaatcg gcttctgcct
    20641 acgcggagca cgcccgggag ccgccacgga tccaggaaaa acgttttgga gcaagggttt
    20701 gtcggccccc gggttcgttg gtcgcggttc gggttcgttc gtcgaaaatc cgggttcgtt
    20761 cgtcgaaaat ccggggttcg ctcgtccgaa tcccgggttc gctcgtcgat ccggtgggtt
    20821 cgttcgtcgt cgactgtcgc agattccgcg acaattccgg tcgactgtcg cggaatctgc
    20881 gacatcacga atccttatgt ggcggaatcc gcgacagttt ggcggacgct gtcgcagaat
    20941 atgcgacaag cagagcccgc atgtcgcgga ttccgcgacg aacctggcgg cgtggtggcc
    21001 tgcaggcggc tggaagcggc gcccgggcgg ctggcccggg ccgaggcata agcgttcgtg
    21061 atggaccttt ggcggcggtc ggttgacccc gggccggcgg cggccatctt ttccgccatg
    21121 gggtggagcc ggcggaagat cctgacgact ggcggtgcgc tggccgcggc cgcgtgcatc
    21181 gcgcgaccgc gctgcgccgc ggccgcgatc gacccggcgg cgctcgtcga acgcgccaaa
    21241 gcgctgccgc ggctgcatac gctgatcgtc gggcggggca gcgagatcgt tctcgcggag
    21301 aggttccgcg gcccggccgc cgaccggccg gtcaacatca agtcgctggc caagacgatc
    21361 atcgcgacgc tcgtcggctg cgccatcgat cgcggcgtgc tcgaggggcc ggaccagagg
    21421 atcacgaccg tgctcggccc gctggtcccg gccgatgccg atccccgcct cgaagaggtc
    21481 acgatcggca acctgctctc gatgcaggcc ggcctcgagc gcacctccgg gcggaactac
    21541 ggcgcctggg tgacgagccc ggactgggtc gcccatgcgc tgagccgccc gttcgtcgcc
    21601 gagcccggcg gccgcatgct ctattcgacg ggcagctcgc acctgctctc ggcgattctg
    21661 accagggcga ccggcgagag cacgctcggc ctcgcccgcg cctggctggg cgagccgctc
    21721 gacatcgaga tcccgccctg ggacagggac ccgcagggca tctatctcgg cggcaacaac
    21781 ttgctgctct cgccccgggc cgtcttccgc ttcggccgga tgatcaacgc cggcggcacg
    21841 ctcgacgggc ggcgcatcgt cgccgagagc tggatcgcca cctcctggcg gccgcgcacc
    21901 gcgtcccgct ggaccggcca ccgctacggc tatggctggt tcatcaccga gaccggcggt
    21961 cacccccact attacggctg gggctatggc ggccagatgc tgcacgtcgt cccgagcctg
    22021 aacctcgtga tcgccatcac ctccgatccg acggtgagat cacgcgaggc ggggcacatg
    22081 gcggggttgc gggcgctggt cgcggcggtg gtcggggaga actagacggg gcgcggtgcc
    22141 cttctcgacg agggcagccg tattcggcgg cgaatggagg tcgtatcgag tcctcgtatc
    22201 ttctcaaatg ggcggaatgc ggtgactgtc tggacgagat gg
//
LOCUS       DAOTFI010000046        22135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2793_length_22135_cov_6.533288, whole genome shotgun sequence.
ACCESSION   DAOTFI010000046 DAOTFI010000000
VERSION     DAOTFI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22135
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2793_length_22135_cov_6.533288"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="P5558_06500"
     CDS             <1..742
                     /locus_tag="P5558_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRY23993.1"
                     /translation="DEAWRLQRVLLESLETAWKRPDEGIWEVRGPPRHFTHSKVMAWV
                     AFDRAVEAVEAFGREGPVEHWRQLRAAIHDDVCANGFDAALNTFVQHYGSTQVDAALL
                     MIPLVGFLAADDPRMVGTVDRIMQELTADGLLRRYAPAESVDGLAGDEGVFLACSFWL
                     ADVLCLMGRLDESRALFHRLLALRNDVGLLAEEYEWRSRRQLGNFPQAFSHIGLIATA
                     HNLGPACGPAHRRAGSRAPVKPRPPGDS"
     gene            840..1565
                     /gene="pyrF"
                     /locus_tag="P5558_06505"
     CDS             840..1565
                     /gene="pyrF"
                     /locus_tag="P5558_06505"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRY23994.1"
                     /translation="MSETRPTFANPVFCAVDRPDLDGAIDLVRVLQPHLGGIKLGLEF
                     FTAQGPDGIRRMGGLGLPIFLDAKLHDIPNTVAGAMRGVVGLPVAMVTLHAAGGSAMM
                     RAAVEIADASARRPLVLAVTVLTSLDEGDLVATGQRGTATEQALRLAELALAAGCHGI
                     VCSPMEVAVMRREFGGVPRLVVPGVRPNSRQDDQKRTLDPAEAVGAGADIVVIGRPIT
                     AEPDPAAAALAIRASLDGVAGAA"
     gene            1567..2229
                     /locus_tag="P5558_06510"
     CDS             1567..2229
                     /locus_tag="P5558_06510"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRY23995.1"
                     /translation="MRGPAVKICGLTRAADRDAAIRAGASWLGFIFYPPSPRSLTPAA
                     AGALVTNRPGAAEAVGVFVDPDDAWLDTVLASAQLDILQLHGKETPERVAAIRARTGR
                     RVMKALAVADAADLDRHRAFVEVADLLLFDAKPPRTPGAIPGGNGLAFDWRLLAGQTI
                     ALPWLLAGGLDETNLSQAVGRTGATAVDVSSGVETAPGIKDPEAIARLLAVARDLQPI
                     TA"
     gene            2244..3476
                     /gene="trpB"
                     /locus_tag="P5558_06515"
     CDS             2244..3476
                     /gene="trpB"
                     /locus_tag="P5558_06515"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRY23996.1"
                     /translation="MATALDPQQRPNSYRTGPDEHGHFGNHGGRFVAETLMPNILELE
                     AAYNEAKTDPAFQAELESWFKDYVGRPSPLYLAQRLTDHLGGARVYFKRDELNHTGAH
                     KINNCVGQILLATRMGKKRIIAETGAGQHGVATATVAARFGLECVIYMGERDIERQQP
                     NVFRMRLLGAEVRPVKSGSRTLKDAMNEALRDWVTNVRDTFYIIGSVAGPHPYPAMVR
                     DFQSIIGTEARAQIQAKEGRLPDLLVAAVGGGSNAMGLFHPFLDDPEVGIVAVEAAGD
                     GVETGRHAAAMTAGQPGVLHGMRSYLMQDNDGQIIEPHSISAGLDYPGIGPEHSWLKD
                     LGRIEVAVATDAEALEAFALCSKLEGILPALEPCHALAEVEKRAPTMRPDQIIIMNLC
                     GRGDKDIYTVAEHMGVRL"
     gene            3490..4341
                     /gene="trpA"
                     /locus_tag="P5558_06520"
     CDS             3490..4341
                     /gene="trpA"
                     /locus_tag="P5558_06520"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRY23997.1"
                     /translation="MSPTRIERRFADLQKEGRAGLITFICGGDPNPQAFQAILAGLPA
                     AGADLIEIGMPFSDPMADGPTIQAGNLRALAAGMTLARLLTQVTAFREKDRTTPLVLM
                     GYYNPIHRYGPARFLDDALSAGVDGLIIVDLPPEEDEELCLPALEKGLNFIRLATPTT
                     DAARLPAVLGNTSGFIYYVSITGITGAASPVADAVGDAVAHLRRHTDLPVAVGFGIRT
                     PEQAAGIARIADAAVVGSALVEEIASRVETSKADSAELIGAVHARVRGLAAAVRAARK
                     DPVEPGP"
     gene            4338..5360
                     /gene="accD"
                     /locus_tag="P5558_06525"
     CDS             4338..5360
                     /gene="accD"
                     /locus_tag="P5558_06525"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRY23998.1"
                     /translation="MNWLTNYVRPRFRALVRSGTKPEVPENLWHQCSSCERMIFHRDL
                     EANQRVCPHCNHHMRVGPEYRFQSLLDEGSWSRIELPKVPLDPLKFRDSKRYADRLKE
                     AQGKTRTQDALEAAHGRIDGLPAVVAVMNFEFMAGSMGAAVGEAFVTAARLAVLQEAA
                     FIIVTSSGGARMQEGAVSLMQMARTTLAVEEVKEAGLPYIVLLTDPTTGGVTASFAML
                     GDIHIAEPGAVIGFAGARVIEQTIRETLPEGFQKAEYLLEHGMVDMVVHRFEMRATMA
                     RLLGLLFRRQHPPSEPAGKPAIVRPAEAVLAPAPAVAPARSGDKPAESATDEAEAAAP
                     APKSGD"
     gene            5389..6669
                     /locus_tag="P5558_06530"
     CDS             5389..6669
                     /locus_tag="P5558_06530"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRY23999.1"
                     /translation="MTAFDSDLILARLQGLHPKSIDLSLARIERLLACLDHPERRVPP
                     VVHIAGTNGKGSTLAMLHAMLEAAGQRVHRYISPHLVRFNERILLHGQPIDESLLADV
                     LDRCERANRGEPITFFEITTAAAFLAFAECPADWLLLETGLGGRLDATNVVDRPALTL
                     ISPVSMDHEAYLGTTLEAIAGEKAGILKPGVPVLAGPQAPAALAVLDARAEVLAAPLR
                     AAGRDWHFAAGDHGFRLDSGMEVLDLPRPGLVGAHQIENAALAAMAGLALGLDADALC
                     QGIRGAIWPARLQRLNGDLAARLPAGAELWLDGGHNPAAGQALALSLDGIAHGRPVDL
                     VLGMLETKDVAAFLAPMRGRIRRLLTVPVPDEHLGRDPAEVAVLARAQGFEAEPAADI
                     EGALGRLAEAGGEPAVVLICGSLYLAGHVLRGRA"
     gene            complement(6674..7255)
                     /locus_tag="P5558_06535"
     CDS             complement(6674..7255)
                     /locus_tag="P5558_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY24000.1"
                     /translation="MMVNEVMRTQPALVDPGSTIAEAARTVRDRDLGCLLVGRDGHLL
                     GLVTDRDLVVRGLANGADPTRERVCDVMSSEVLSCFDDQPVESVRAIMAEQGVRRLPV
                     VDRWGRLTGVVSLSDVQGGGVRNIKPHAVTFYKHLVDGSGTVHEVPLKTVYVAKVDDE
                     DDATAVATAIFERYWGPARWKGTADGCRIDRGS"
     gene            complement(7392..7895)
                     /locus_tag="P5558_06540"
     CDS             complement(7392..7895)
                     /locus_tag="P5558_06540"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRY24001.1"
                     /translation="MSARLEVQGNVVESGHAERVGGHDHFVRDLEARRRQAEAIVDSV
                     HWTWTSLRRAMQRLAGRNLPERGPAANDNRAQGGVGAFGLGLWPVRRIGRSILDSYAK
                     WRRRRIAIDELRTLDDRVLADIGLRRGQIELAVDGLLRRRGESWERPVGRDRAPNGCR
                     EDLPLAA"
     gene            complement(8286..9236)
                     /locus_tag="P5558_06545"
     CDS             complement(8286..9236)
                     /locus_tag="P5558_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HRY24002.1"
                     /translation="MVRREAAADGRIAYAVVTTGIYCRPSCPSPTPLRLNVRIFPNNL
                     AAEVAGFLPCKRCRPTMASPLGWHVTAVGKACAILGTSERAPALAAVAGAVGISRFHF
                     HRVFKEVLGTTPGKYFEAVRSRRLARGLASDRPVTETIHNAGYDSVSRAYGHARERLG
                     MTPATRHAGGVGARVGFTIVEQVTGQVLVAITNEGVCAIEFGDDAAELEAWLRRDLPA
                     AVIGRLATDTIARIAAAVRRAELPSLARDLPSEIREVAFRARLGNLLAPGFGRRHQSR
                     VERSRAARPAGLAAMSGAAPIFAGAGKLRYASGRRGRPTF"
     gene            complement(9381..10436)
                     /gene="dctP"
                     /locus_tag="P5558_06550"
     CDS             complement(9381..10436)
                     /gene="dctP"
                     /locus_tag="P5558_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY24003.1"
                     /translation="MTKKLACGLLAAALLAGTALPTNAAEYVLKYGHPGPVGPDSDDH
                     VAGEFLKYFLETQSNGRIEVQIFPGSQLGSFREMVEQVNANTLEVAHTALGGLVGFMP
                     ELQVIELPYVVRDDAVAECMIQGPFFEDMRETFMAKSGNVNLIGVGNTGRFRSFFTAN
                     KLVKSAEDLKGIKMRVINSQLPVEMMQFYGANPTPIAWGELYTSLATGVVEGTKNAVT
                     DIIPNKMEEVVRYATMDQHAYLWGFMAVSQDFLDSLPDDLRAMVVSGVRQMADVQLEF
                     NKGAEARQSKVFTDGGGEIYVPNEEEMATFRAVKEPMGEWYKAQFGDEWLNKFTSAAT
                     ECEEEVDLQLAKWGSRE"
     gene            complement(10487..11776)
                     /locus_tag="P5558_06555"
     CDS             complement(10487..11776)
                     /locus_tag="P5558_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY24004.1"
                     /translation="MALVFFWLLLALMALGVPIVFALVMAPVFGILIQDKLVFLSMLP
                     QRIFSGINQFPLLAIPMFILAGEVMNHGGITLRLVTFAKTLVGHFRGGLAQVNILSSM
                     LFAGLSGSAVADTSALGSMLIPAMEKDGYTRRFAAAVTAASSVIGPVIPPSIIMVVYA
                     YIMGVSVAALFAAGFVPGIMMGGGMMIMTSFLARRRNYPKSERRAPPKEVATAFGHAF
                     LPLLTPVIILGGILSGVFTPTEAAAAAVAYALILSLLVTRTLEIARIPDMLYRAGIAS
                     SSILFVIGAATLFGWVTSLSGVPTAVSGILLGVSDNPWVVLFLVNILLLIVGMFFDAG
                     PAILILGPLLGPTMQQLGIDPLHFAIVMCVNLTVGLATPPMGLVLFVASTLTRLSIVE
                     IAKDMWPFLIVHIMVIFLIAYFPFLTMTLPRLFGFGV"
     gene            complement(11783..12337)
                     /locus_tag="P5558_06560"
     CDS             complement(11783..12337)
                     /locus_tag="P5558_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY24005.1"
                     /translation="MTGLAELTNGLGRFNATLLVVCRWLVILIVAALAVILSVAVFRR
                     YALNNAIPWSEEAAKYLMVWLAFMGSPIALRHGGHVNVDLAVAWMRGRARQVVFLLAH
                     LIVIGAAAMLVWQGFLFAKQGVRQVASSFQMSMVWVYAAVPLGAGLMLLVSVEQALRA
                     LNGIFDPLRGYVEDHESAIDEIRE"
     gene            12561..13820
                     /locus_tag="P5558_06565"
     CDS             12561..13820
                     /locus_tag="P5558_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY24006.1"
                     /translation="MLSTFDRRHALKALGVTAAGAATGFPAPFVRAQGATIQMVSHRY
                     PALEHYAAKMQSALPGVEVNTQLMPFDKANELATIALSSGASTFDIVYANDSTVLKYA
                     KNGWLRPIDDLFEKYKDEFNFADYPESVLDSYRYKGELFVLPHTANVMLFFYRKDLFE
                     EAGAEVPTTMEEYEALAAQFNSPARAGTISCQKPVDASLNEIHWYMSTIGDGWFDENL
                     QPVFNSPNGVAAIETLKRITANAQRGFTSAANDECTIALQQDLAVMGLQWATRCASMD
                     DPAKSRVVGLIDWAAAPGGHTRLGGDGYGISAYTSQDPDQLFRILAISSSYESMSEAA
                     SMMVPPRSSILNDPAVAEKYRHFPGVLAALETAQPFPSLPEFYELGETITRRVHQAIT
                     DEMEVQAALDAAAAETIGLLRDRGYDL"
     gene            13922..14737
                     /locus_tag="P5558_06570"
     CDS             13922..14737
                     /locus_tag="P5558_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY24007.1"
                     /translation="MTLVLLYPLGYAFYLSLFNYYLGAEPIFIGLGNYIALLQDERFW
                     SSLRTTLLLVGASVSLQFVLGLAVAFGLYKLTFATKALNVLLFLPHVVTPVVAALFLR
                     WIFMGRFGLIDAILIGLNIFPPDWLGSPNWARLVVVLAESWQFTPFMIIVLYAGLNTV
                     DQSQIESARMDGAGNWMLLTRVMLPAIRPLILFVLAIRIMDSFRFFDTIYVLTAGGPG
                     TATETVTIYTYALGFRLLEVGKASALGVLTLLITATAIALVIAFLYRREKGAF"
     gene            14737..15561
                     /locus_tag="P5558_06575"
     CDS             14737..15561
                     /locus_tag="P5558_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY24008.1"
                     /translation="MKPSLHTQMLLFLALCAFTAINLTPIFWAFMVSIKQPVDAFAAP
                     PMLIFTPTFEFHYSVWVERGFWRFLLNSVIIAVAVVCISVPIGTMAAYALSRMEGAKA
                     RGILFGLLAMRMFPHILLAIPFFVIASAIDLIDTYLAMVLALVAINQPFTVWLMRSFF
                     LDVPRELDEAATIDGASLWQTFTRVALPVVRPGLWVTSLFSLLLAYNEFLFALVLTGS
                     STKTLPVAIAEYGAEDINYWSLSAAAAIGIMLPIVLFMAFMQRHLVRGLAMGAVKG"
     gene            15569..16654
                     /locus_tag="P5558_06580"
     CDS             15569..16654
                     /locus_tag="P5558_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY24009.1"
                     /translation="MTEAILPAGAIDCDVHPVVPGIAALVPYLDAYWQEMIDVRGIEG
                     FESRAYPPMAPMSARADWRGPDGRAAESVEPVRRQLFDQWGEGGAILNCLYGIQTIHD
                     LSLAAAMARAVNDWLAAEWLAIEPRFRASIVLPLQSPELAVEEIERRAADKRFVQVLA
                     LVMGEQPLGQRAYWPVWAAAERHGLPLAIHTGSAYRRAITALGWPSSWLEDYVDQAQA
                     FQAQVGSLVSHGVFNRFPGLKVVLSESGITWLPAFLWRFSKNWRGLRIEIPWVERPPI
                     EIVRDHFRLTLQPIDAPDGATFERIIDQLGSDEMLLFSTDYPHWQLDGDQPMPDWLPA
                     GTRRKIAVDNPRATYPRLGEVPSGGDR"
     gene            16651..17787
                     /locus_tag="P5558_06585"
     CDS             16651..17787
                     /locus_tag="P5558_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY24010.1"
                     /translation="MSLDTVDDVLVESRGEARAERTRPVRSTIVDCDVHPTVRTAKDL
                     EPYLARRWWDHFTTYGLRPKHGNATADPYPKAQPRAARRDAWAPNGDQPGSNLAFMRE
                     HYLDPYGVECGILSPLNPTGQGDQNIDFSIAMTHAVNDWQLDVFTRLEPRFKASIVIP
                     YEDGEASAREIELRAGTADFAQVLFLTRTSEPIGKRRYWPIFEAAAHHGVPVALHVFG
                     YSGHPVSGTGWPSYYIEEMTGHSAACQAAVASLVLEGVFERFPELKVVIIEGGFGWLP
                     SLTWRLDRHWQKLGAEVPGCRRPPSEYIREHIWITTQPIEEPEDTADLIQTMEWIGWD
                     RLLFATDYPHWDFDDPMRALPRGLDPANRRKIEAENARALFKLG"
     gene            17787..18200
                     /locus_tag="P5558_06590"
     CDS             17787..18200
                     /locus_tag="P5558_06590"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRY24011.1"
                     /translation="MARHIVATAREIPEGGRKRVVINSRPIAVFHSKGEYFALYDTCP
                     HEGGSLSAGKLTGLVQSDGPGDYRLCRKGEIVRCPWHGWEFDLRTGQSYCDPRRMRVK
                     SYEAGTTRPSEAVEGPYKATTFPVTVDDEYVFVEV"
     gene            complement(18207..19112)
                     /locus_tag="P5558_06595"
     CDS             complement(18207..19112)
                     /locus_tag="P5558_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY24012.1"
                     /translation="MQRLLRESLDGFAADLGGTKIAAARVARGIVEERLQTATDGKAG
                     PDAQLATIAGLLQKLGHAPGAPLGVAVAGRIDGEESWHAVNRGILEAVQGFPLASALE
                     RIVGAVTCCNDAAAAAFAEAHFGAGRGCRHFAYLTVSTGVGGGIVLGGQLIQSQNGLA
                     GHVGFVSSPFGRNACGSGRYGTVESTAAGRAIAAEAAAQGHAEADARAVFAAAAQGQA
                     WAEAIIEGSAKAVATLIADLATILGLDRVAIGGSVGLADGYLDRVRTALAEEPELFRV
                     ELARAELGHDSALLGALALQRLQAG"
     gene            complement(19112..19795)
                     /locus_tag="P5558_06600"
     CDS             complement(19112..19795)
                     /locus_tag="P5558_06600"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF002231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HRY24013.1"
                     /translation="MSLLDTLRGGLVVSCQPVDDGPMDRPEIVAAMALAAVAGGAAGL
                     RIEGIDNLRAVRPHVTVPIIGIVKRDLEVSPVRITPLIADARGLVAAGADIVAYDATI
                     RPRPDAAEAILEAIRDGRALAMADCATAEDGRRALAAGAAVLGTTLSGYTAETAGRGD
                     GVDLELIRAFRELGGFVMAEGRVNTPELAASAIRAGADAVTVGTALTRLELVTGWFEA
                     AVAKAAGRA"
     gene            complement(19822..21096)
                     /locus_tag="P5558_06605"
     CDS             complement(19822..21096)
                     /locus_tag="P5558_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY24014.1"
                     /translation="MILATLGALWLVFVLVGVPIGVAMIFVSMGYFYWTGMGISFAVQ
                     RMVDGLNSFPLLAVPLFILAAGILNSAGITNHLFGFARALVGHVTGGLGHVNVLASLF
                     FSGMSGSALADAGGLGKMEIEAMREAGYDDDFAGSVTAASSMIGPLVPPSITMVLYGV
                     IANVSIGKLFLGGIIPGVLCAGALMVMVYVLARRRDYPRDPRQSVGQIGSLFIRSLPA
                     LLTPVVIVGGIFSGLFSPTEAAAVTVLYAIAIDVIFYRELTFRRLWDAIYETTVTSAA
                     IALIVAGVALLSFILAREQAPQQIASLFLGIADGPLTFLIAVNLMIFVLGCFIEALVI
                     LLIIVPILVPIAAGFGIDPVHFGIIVVLNLMISILTPPMGMALFVVAQVGNIPYQRLA
                     RAILPWLIPPFVVLILVCAFPILSTGLPSLMD"
     gene            complement(21093..21572)
                     /locus_tag="P5558_06610"
     CDS             complement(21093..21572)
                     /locus_tag="P5558_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY24015.1"
                     /translation="MSEPRKYSLEGSIASVLFIALIGVVLIQVFGRTPLFRGPVWTEE
                     AARWLWVWMAFIAIGEVERQDGQLKMAFLADALPATVRRALYTVIDLVYLGIMAHLCW
                     IGFRTIQRTWSNISVTLPTSDALLYASAFVASFFIIHRILRRLLGRNGRAADRSPAL"
     gene            complement(21647..>22135)
                     /gene="dctP"
                     /locus_tag="P5558_06615"
     CDS             complement(21647..>22135)
                     /gene="dctP"
                     /locus_tag="P5558_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY24016.1"
                     /translation="DMKGLRLRTPNVPFLIAYAENTGATPAPVAFQEVYLALQTNQVD
                     AEENPLPTIDAMKFYEVQSHIALTNHFVASAAVQMSADTWADLSEEEQGWVRAAVEAG
                     GELNDSLTMQAEKDLLATFEERGLTITRPDLEPFRLAMQPYYDTLEEQFGEGSIAAVT
                     GD"
BASE COUNT         3618 a         7690 c         7313 g         3514 t
ORIGIN      
        1 ggacgaagcg tggcggctcc agcgcgtgct gctcgagtcg ctggagacgg cctggaagag
       61 gccggacgag ggcatctggg aggtgcgcgg cccgccccgc cacttcaccc attccaaggt
      121 catggcctgg gtcgccttcg atcgcgcggt cgaggcggtc gaggccttcg gccgggaagg
      181 gccggtcgaa cactggcgcc agttgcgtgc ggcgatccac gacgacgtct gcgccaacgg
      241 gttcgatgcc gcactgaaca cgtttgtcca gcactatggc agcacgcagg tcgatgccgc
      301 cctgctgatg ataccgctcg tcggctttct tgccgccgac gatccgcgca tggtcggcac
      361 ggtcgaccgc atcatgcagg agctcacagc cgacggcctg ctccgccgct atgcgccggc
      421 cgagtcggtc gacggtcttg ccggcgacga gggcgtcttt ctcgcctgct ccttctggct
      481 cgccgacgtg ctctgcctga tgggccggct cgacgagtcc cgggctctct ttcatcgtct
      541 gctggcgctg cgcaacgatg tcggcctgct ggccgaggag tacgagtggc ggagccggcg
      601 ccagctcggc aatttcccgc aggccttctc gcatatcggc ctgattgcca cggcccacaa
      661 tctcggcccg gcgtgcggcc ccgcgcatcg ccgcgccgga tcgcgcgcgc ccgtcaagcc
      721 gcgtccgccc ggggattcct gagcgccaac cgtcatgccg ttgttcgtgg cttggtcaag
      781 cgtcatcgcc tctgctatag ctccgctgct ctcggccgaa cgccaggaaa cgcacgccct
      841 tgtccgaaac acgcccgacc ttcgccaatc ccgtcttttg cgccgtcgac cgccccgacc
      901 tcgacggtgc catcgatctc gtgcgggtct tgcagccgca tctgggcggc atcaagctcg
      961 gcctcgagtt tttcacggcg caggggccgg acggcatacg ccggatgggc ggcctgggcc
     1021 tgccgatctt cctcgacgcc aagctgcacg acatcccgaa cacggtggcc ggcgccatgc
     1081 gcggcgtggt cggactgccg gtcgccatgg tgacgctgca cgccgccggc ggctcggcca
     1141 tgatgcgcgc cgccgtggag atcgccgatg ccagcgcccg ccggcctctg gtgctggcgg
     1201 tgacggtgct caccagcctc gacgagggtg atctcgtggc gacaggccag cgcggcacgg
     1261 ccaccgagca ggcgcttagg ctggccgagc tggcgctcgc tgccggttgc catggcatcg
     1321 tctgctcgcc catggaggtg gccgtcatgc gccgcgagtt cggcggcgtg ccccggctgg
     1381 tggtgccggg cgtgcggccg aattcgcgtc aggacgacca gaagcgcacg ctggatccgg
     1441 ccgaggcggt cggtgccggc gccgacatcg tggtcatcgg ccggccgatc accgccgagc
     1501 cggaccccgc ggccgcggcc ctggccattc gcgccagcct ggatggcgtg gccggagcgg
     1561 cctgacgtgc gcggcccagc tgtcaagatc tgtggcctga cccgggccgc cgaccgggat
     1621 gccgcgatcc gggccggtgc gagctggctc ggcttcatct tctatccgcc ctcgccccgc
     1681 tcgctgacgc cggcggcggc cggagccctg gtcacgaatc ggccgggtgc tgccgaggcg
     1741 gtcggcgtct tcgtcgatcc cgacgacgcc tggctcgata cggtgcttgc ctcggcccag
     1801 ctcgacatcc tgcagctgca tggcaaggag acgcccgagc gcgtggcggc gatccgcgcc
     1861 cgcacgggcc gacgcgtgat gaaggcgctg gccgtggccg atgcagccga tctcgatcgc
     1921 caccgggcgt tcgtcgaggt tgccgacctc ctgctcttcg acgcgaagcc gccccgcaca
     1981 ccgggcgcca tcccgggcgg caatggcctc gccttcgact ggcgcctgct cgccgggcag
     2041 acgatcgctc tgccatggct cctggccggc ggcctggacg agaccaatct ttcccaagcc
     2101 gtggggcgca ccggcgccac ggcggtcgac gtctcctcgg gcgtcgagac cgcacccggc
     2161 atcaaggatc cagaggcgat cgcccggctc ctcgccgtgg cgcgcgacct gcaacccatc
     2221 accgcttgat tagggcagca ttcatggcca ccgcgctcga cccacagcag cgcccgaaca
     2281 gctaccgcac cggccccgac gagcatggcc atttcggcaa tcacggcggc cggttcgtcg
     2341 ccgagacgct gatgccgaac atcctcgagc tcgaggccgc ctacaacgag gcgaagacgg
     2401 acccggcttt ccaggcggag ctcgagagct ggttcaagga ctatgtcggc cgcccgtcgc
     2461 cgctctatct ggcgcagcgc ctgaccgacc atctcggtgg cgccagggtc tacttcaagc
     2521 gtgacgagct gaaccacacg ggtgcccaca agatcaacaa ctgcgtcggc cagatcctgc
     2581 tcgcgacacg catgggcaag aagcggatca tcgccgaaac cggcgccggg cagcacggcg
     2641 tcgccaccgc cacggtggcc gcccgcttcg gcctcgaatg cgtgatctac atgggcgagc
     2701 gcgacatcga gcggcagcag cccaacgtct ttcgcatgcg tctcctcggg gccgaggtgc
     2761 gaccggtcaa gtccggctcg cgcaccctca aggatgccat gaacgaagcc ctgcgggact
     2821 gggtcaccaa cgtccgtgac accttctaca tcatcggctc ggtcgccggc ccgcaccctt
     2881 atccggccat ggtccgggac ttccagagca tcatcggcac cgaggcaagg gcgcagatcc
     2941 aggcgaagga ggggcgcctg ccggacctcc tggtcgccgc ggtcggcggc ggctccaatg
     3001 ccatggggct gttccatccc ttcctcgacg accccgaggt cggcatcgtc gcggtcgagg
     3061 ccgccggcga cggggtcgag acgggccgcc atgccgccgc catgaccgcc ggccagcccg
     3121 gcgtgctgca cggcatgcgc agctacctga tgcaggacaa tgacggccag atcatcgagc
     3181 cgcactcgat ctcggccggg ctcgactatc ccggcatcgg tcccgagcac tcctggctca
     3241 aggacctggg gcggatcgag gtggcggtcg ccaccgacgc cgaggcgctc gaggccttcg
     3301 ccctctgctc gaagctcgag ggcatcctgc cggcgctcga gccttgtcat gcgctggccg
     3361 aggtcgagaa acgcgcgccg acgatgcgcc ccgaccagat catcatcatg aatctctgtg
     3421 gccgcggcga caaggatatc tacaccgtgg cagagcacat gggcgtgcgg ttgtgagcga
     3481 gggactgcga tgagcccgac ccgcatcgag cgacgcttcg ccgatctcca gaaagagggc
     3541 agggcggggc tgatcacgtt catctgcggc ggcgatccca acccccaggc cttccaggcg
     3601 atcctcgccg gcctgccggc ggccggtgcg gatctcatcg agatcggcat gccgttctcc
     3661 gatccgatgg ccgacggccc gaccatccag gcgggcaatc tccgggcact cgccgccggc
     3721 atgacgctcg cccgcctcct gacccaggtc acggcctttc gcgagaagga caggacgacc
     3781 ccgctcgtgc tgatgggcta ctacaacccg atccaccgct atggcccggc acggttcctc
     3841 gacgacgcgc tgtccgccgg cgtcgacggg ctgatcattg tcgacctgcc gcccgaggag
     3901 gacgaggagc tttgcctgcc ggcgctcgag aaggggctga acttcatccg cctcgccacg
     3961 ccgacgaccg acgcggcccg actgccggcg gtgctcggca atacctcggg cttcatctac
     4021 tacgtctcga tcaccggcat caccggcgcc gcctcgcccg tggccgatgc ggtcggcgat
     4081 gcggtggccc atctccggcg ccacaccgac ctgccggtcg ccgtcggctt cggcattcgc
     4141 acccccgagc aggctgccgg catcgcccgg atcgcggatg ccgccgtggt tggctcggcc
     4201 ctggtggagg agatcgcgag ccgggtcgag accagcaagg cggatagcgc cgagctgatc
     4261 ggcgccgtcc acgcgcgggt gcgcggcctc gccgccgcgg tgcgcgcggc gcgcaaggac
     4321 ccggtcgagc ccggcccatg aactggctca ccaactatgt gcggccgcgt tttcgcgccc
     4381 tcgtgcgcag cggcaccaag cccgaggtgc cggagaacct ctggcaccag tgctcgtcct
     4441 gcgagcggat gatcttccac cgtgatctcg aggccaacca gcgggtctgc ccgcactgca
     4501 atcatcacat gcgggtcggc cccgagtatc gtttccagag tctcctcgac gagggcagct
     4561 ggagccggat cgagctcccc aaggtgccgc tcgacccgct caagttccgg gattccaagc
     4621 gctacgccga ccggttgaag gaggcgcagg gcaagacccg cacccaggac gcgctcgagg
     4681 cggcgcacgg ccggatcgac ggcctgccgg cggtcgtcgc ggtcatgaac ttcgagttca
     4741 tggcgggctc gatgggggcc gccgtgggcg aggctttcgt caccgcggcc cggctcgccg
     4801 tcctgcagga ggcggccttc atcatcgtca cctcctcggg cggcgctcga atgcaggaag
     4861 gcgccgtctc gctgatgcag atggcgcgca ccacgctggc cgtcgaggag gtgaaggagg
     4921 ccggcctgcc ctatatcgtc ctgctcaccg acccgacgac cggcggtgtt acggcctcct
     4981 tcgccatgct cggcgacatc cacatcgccg agcccggtgc ggtgatcggc ttcgccggcg
     5041 cccgggtgat cgagcagacc atccgcgaga ccctgcccga gggcttccag aaggccgaat
     5101 acctgctcga gcacggcatg gtcgacatgg tcgtccaccg tttcgagatg cgcgccacca
     5161 tggcacggct gctcggcctc ctcttccgcc ggcagcaccc gccaagcgag ccggcgggaa
     5221 agcccgcgat agtccggccg gccgaggctg tcctggcccc ggcacccgcc gtggctccgg
     5281 ccaggagcgg cgacaagccg gcggagtcgg ccacggacga ggcggaagcg gccgctccgg
     5341 cgcccaagag cggcgactag gcgctcgata tctcagggga gtcggtccat gaccgccttc
     5401 gacagcgacc tcatccttgc ccggctgcag ggcctccacc cgaagagcat cgacctctcg
     5461 ctcgcccgca tcgagcgcct gctcgcctgt ctcgaccatc ccgagcggcg ggtgccgccg
     5521 gtggtgcaca tcgccggcac caacggcaag ggctcgaccc tggccatgct gcacgccatg
     5581 ctcgaggccg ctggccagcg cgtgcatcgc tacatctcgc cgcatctcgt ccgcttcaac
     5641 gagcgcatcc tgctccatgg gcagccgatc gacgagagcc tgctcgcgga tgtgctggac
     5701 cgctgcgagc gcgccaaccg gggcgagccg atcactttct tcgagatcac cacggccgcg
     5761 gccttcctcg ccttcgccga atgcccggct gactggctgc tgctcgagac cgggctcggc
     5821 ggccggctgg atgcgaccaa tgtggtggat cggccggcgc tcaccctgat ctcgccggtc
     5881 tcgatggacc acgaggccta tctgggcacc acgctcgaag ccatcgccgg cgagaaggcc
     5941 ggcatcctca agccaggggt gccggtgctg gcgggaccgc aggcgccggc ggcgctcgcc
     6001 gtgctggacg cccgggccga agtgctcgcc gcacccctgc gcgctgccgg tcgcgactgg
     6061 cacttcgccg ccggcgacca tggctttcgc ctcgacagcg gcatggaggt gctcgacctg
     6121 ccgcggcccg gcctggtcgg cgcgcaccag atcgagaacg cggcgcttgc cgccatggcg
     6181 gggcttgcgc tcggactgga cgcggacgcc ctctgccaag gcattcgggg tgccatctgg
     6241 ccggcccggc tgcagcggct gaatggcgat ctcgccgcac gcctgccggc gggggccgag
     6301 ctatggctgg acggcggtca caaccctgcc gccggacagg cgctggcgct gagtctcgac
     6361 gggatcgccc atggccggcc ggtcgatctc gtgctcggca tgctcgagac caaggacgtg
     6421 gcggccttcc tcgcccccat gcgggggcgc attcggcgac tcctgacggt gccggtgccg
     6481 gacgagcacc tcggccgcga tccggccgag gtggcagtgc tcgcccgggc acagggcttc
     6541 gaggccgagc cggcggcgga catcgaaggg gccctcggcc ggttggccga ggcgggcggc
     6601 gagccggccg tcgtcctgat ctgcggctcg ctctacctcg ccggccacgt gctccgcggc
     6661 cgcgcgtagc ccgtcaactg ccgcggtcga tccggcagcc gtcagccgtc cccttccatc
     6721 gggcgggacc ccagtaccgc tcgaaaattg cggtggcgac ggcggtggcg tcgtcctcgt
     6781 catccacctt ggcaacgtag actgtcttga gcggcacctc atggaccgtt ccgctgccgt
     6841 cgacgaggtg cttgtagaag gtcaccgcgt gcggtttgat gttcctcaca ccgccgccct
     6901 gcacgtcgct cagggacacg actccggtca gccgccccca ccggtcgacc acgggcagcc
     6961 ggcggacccc ctgctccgcc atgatcgccc tcacgctctc gaccggctgg tcgtcgaaac
     7021 aggacagcac ctcgctcgac atcacgtcgc acacccgctc gcgggtcggg tccgcgccat
     7081 tggccagacc gcgcacgacg aggtcgcgat cggtgacgag ccccagcaaa tgcccgtccc
     7141 gtccaacgag caggcagccg aggtcgcggt cgcgcacggt ccgcgccgct tcggcgatcg
     7201 tcgagcccgg atcgaccagg gcgggttgcg ttctcatgac ctcattgacc atcatggcga
     7261 tcttctcctc ccgaggcgtg tcgtgatttt tacgataccg cccaggccac atgcttcttt
     7321 ggccgcgacc ctgccttccg atcgaggcgg cgacaccaat cagccgaggt gtcgccgccg
     7381 gacctcgccg gctacgctgc cagcggcagg tcctcccggc acccgttcgg tgctcggtct
     7441 cgtccgaccg gccgttccca ggactcgccg cgacgccgca gcaggccatc gacggccagc
     7501 tcgatctggc cgcgccgcaa tccgatatcg gccaggacgc ggtcgtccag cgtgcggagc
     7561 tcgtcgatgg caatcctgcg ccggcgccat ttcgcgtagg aatcgaggat cgagcggccg
     7621 atccgccgca ccggccacag ccccaacccg aaggcgccga cgccaccctg tgctcgattg
     7681 tcgttggccg cgggcccgcg ttccggcagg ttccgccctg caaggcgctg catggcacgc
     7741 cgcaagctcg tccaagtcca gtggacgctg tcgacaatcg cttccgcctg ccgtcggcgc
     7801 gcctccaggt cacgcacaaa atggtcgtgc ccgccgaccc gctcggcatg accgctctcg
     7861 accacgttcc cttgcacctc taatcgcgcc gacatcttca cactctcctt gtttatgaaa
     7921 ctcggtatta catgtagtag caggtttatt tattgcaata gagcttttat tcttcttggt
     7981 atgaagaaaa acttatactc tgttgcgatt gacaatgcct tttgttattt gaaatgacaa
     8041 gcgtcggtct agcatggtcg cgtgaattag ttgtagagat cattcggtct ttggagatcg
     8101 cgtcggttgc ggtccagcgt ccaactcggc ggatcactcg ccgggaacga atccatcagt
     8161 gtcttgtcga tgaggtcgtc ccgacgtccg ctccttcccg atcatggtcg cttcctgttt
     8221 ccgccccgca gacgaaaccg aaccatcctc gaatggccgc atcgggggct ctccttattc
     8281 cttgctcaaa aggtgggccg accccgccgc cccgacgcgt agcgaagctt gccggcgccc
     8341 gcgaaaatcg gcgccgctcc cgacatggcg gccagccccg ctggccgcgc cgccctggac
     8401 cgctcaaccc ggctctggtg gcgccggcca aagcctggcg cgaggagatt cccgagccgt
     8461 gcccgaaatg ccacctcgcg tatctcggat ggcaagtccc gggccagcga cggtagttcg
     8521 gcgcggcgca cagcggcagc gatcctggct atcgtgtctg tggcgagacg cccgatcacc
     8581 gccgccggca gatcgcggcg caaccacgcc tccagctccg cggcgtcatc gccgaactcg
     8641 atggcgcaga caccttcatt ggtgatggcc accagcacct gcccggtcac ctgctcgaca
     8701 atggtgaacc cgacccgggc gccaacgcca ccggcgtgcc gtgtcgcggg cgtcatgccg
     8761 agcctctcgc gagcgtgccc gtaggcccgc gagacggagt cgtagccggc attgtggatc
     8821 gtctcggtca ccggtcgatc agacgccaga ccccgggcga ggcgccgcga acgcacggcc
     8881 tcgaaatact tgccgggagt ggtgcccagc acctccttga agacccgatg gaaatgaaag
     8941 cggctgatgc ccacggcacc ggcgacagcc gccagggccg gcgctcgctc actcgtgccg
     9001 aggatggcgc aggccttgcc aacggcggtg acgtgccagc cgagcggcga ggccatcgtc
     9061 ggccggcagc gcttgcacgg caagaacccc gcgacctcgg ccgcgagatt gttggggaag
     9121 atgcgaacgt tgagcctgag cggtgtcggc gacggacatg acggtcggca atagatgccg
     9181 gtcgtgacca ccgcgtaggc gatgcgcccg tccgcggccg cttcacgacg caccaccgcg
     9241 tcccagcacc tgtccccgtc agcgatcacg gcctgccccc cacccttcaa gacatccctc
     9301 gagtgtccga tagggaggag ggcagtgcaa aggccgaaat ctactcgttt attgctttct
     9361 cactcgtcct ggcatccgga tcactcgcgg ctgccccact tcgccagctg gagatccacc
     9421 tcctcctcgc actcggtggc ggcggaggtg aacttgttca gccattcgtc gccgaactgg
     9481 gccttgtacc actcgcccat cggctccttg acggcgcgga aggtcgccat ctcctcttcg
     9541 ttcggcacgt agatctcgcc gccgccgtcg gtgaagacct tgctctgccg cgcctcggcg
     9601 cccttgttga actcgagctg cacgtcggcc atctgccgga cgccgctgac caccatggcg
     9661 cggagatcgt cgggcaggct gtcgaggaag tcctgactca ccgccatgaa gccccagaga
     9721 taggcgtgct ggtccatggt ggcgtagcgg acaacctcct ccatcttgtt ggggatgatg
     9781 tcggtcaccg cgttcttggt gccctcgacc acacccgtcg ccagcgaggt gtagagctcg
     9841 ccccaggcga tcggcgtcgg gttggcgccg tagaactgca tcatctcgac cggcaattgc
     9901 gagttgatca cccgcatctt gatgcccttg aggtcctcgg cactcttcac cagcttgttg
     9961 gcggtgaaga agctcctgaa gcgcccggta ttgccgacgc cgatcagatt gacgttgccc
    10021 gacttcgcca tgaaggtctc gcgcatatcc tcgaagaacg gcccctggat catgcattcg
    10081 gcgaccgcgt cgtcgcgcac cacatagggc agctcgatca cctgcagctc gggcatgaag
    10141 ccgacgagac cacccagcgc cgtgtgcgcc acctcgagcg tgttggcgtt gacctgctcg
    10201 accatctcgc ggaacgagcc gagctgcgag cccgggaaga tctgcacctc gatgcgcccg
    10261 ttggactgcg tctcgaggaa gtatttcagg aattctccgg cgacatggtc gtcgctgtcg
    10321 gggccgaccg gccccgggtg accgtatttg agcacgtact cggcggcatt ggtcggcaag
    10381 gccgtgccgg cgagcagggc tgccgcgagc aggccacaag cgagtttctt ggtcatgtgc
    10441 aagtctccct ggatattggc gcccagccgg gcgctcggtg acgccttcag accccgaagc
    10501 cgaaaagacg tggtaaggtc atggtaagga aggggaagta ggcgatcaag aagatcacca
    10561 tgatgtgtac gatcaggaac ggccacatgt ccttggcgat ctcgacgatc gagagccgtg
    10621 tcagcgtcga ggcgacgaag aggacgaggc ccatgggcgg cgtggcgagg ccgaccgtca
    10681 ggttgacgca catgacgatg gcgaagtgca ggggatcgat gccgagctgt tgcatggtcg
    10741 gcccgagcag cggcccgaga atgaggatcg cgggaccggc gtcgaagaac atgccgacga
    10801 tcagaagaag aatgttgacg aggaagagca cgacccacgg gttgtcgctc accccgagca
    10861 ggatgccgct caccgccgtt ggcacgcccg agagcgaggt gacccagccg aagagcgtgg
    10921 cggcaccgat gacgaaaaga atgctcgagg aggcgatccc ggcccggtag agcatgtccg
    10981 ggatacgggc gatctcgagc gtgcgggtga cgaggaggct gaggatcagg gcataggcga
    11041 cggcggcggc cgcggcctcg gtcggcgtga agacaccgga gaggatgccg cccaggatga
    11101 tgaccggggt caggagcggc aggaaggcgt ggccgaaggc ggttgccacc tccttcggcg
    11161 gtgcccgccg ttcgctcttc ggataattgc gccggcgggc gaggaaggag gtcatgatca
    11221 tcatgccgcc gcccatcatg atgccgggga cgaagccggc ggcgaagagc gccgcgaccg
    11281 agacacccat gatgtaggcg tagacgacca tgatgatcga gggcgggatc accgggccga
    11341 tcaccgatga cgcggcggtg acggcggccg cgaaccggcg ggtgtagccg tccttctcca
    11401 tggccgggat gagcatcgag cccagggccg aggtgtccgc cacggccgag cccgagagcc
    11461 cggcgaagag catgctggag aggatgttga cctgggcgag gccgccccgg aaatggccga
    11521 ccagcgtctt ggcgaaggtg acgagccgaa gcgtgatgcc gccgtggttc atcacctcgc
    11581 cggcgaggat gaacatcggg atggcgagca gcgggaactg gttgatgccg gagaagatcc
    11641 gctgcggcag catcgagagg aagaccagct tgtcctgaat caggatgccg aagaccggcg
    11701 ccatgaccag cgcgaagacg atcggcacgc cgagcgccat gagggcgagg aggagccaga
    11761 agaagaccag ggccatggcc ggctactcgc gaatttcgtc gatggcggat tcgtggtctt
    11821 cgacatagcc gcggagcgga tcgaagatgc cgttcagggc gcggagcgcc tgctcgacag
    11881 agaccagcag catcaacccg gcgccgagcg gcaccgccgc gtagacccag accatcgaca
    11941 tctggaagct cgacgccacc tggcgcaccc cctgcttggc gaagaggaag ccctgccaga
    12001 ccagcatggc cgccgccccg atgacgatca ggtgcgccag caggaagacc acctgcctgg
    12061 cccggccccg catccaggcg acggccaggt cgacattgac gtggccgcca tggcgaaggg
    12121 cgatgggcga gcccatgaag gcgagccaca ccatcaggta cttggcggcc tcctcggacc
    12181 agggaatggc gttgttgagc gcatagcgcc gaaagaccgc gacactcagg atcacggcga
    12241 gggcggcgac gatcaggatc accagccagc ggcagacgac gagcagcgtg gcattgaaac
    12301 gccccaggcc gttcgtcagc tccgccagac ccgtcacccg ccggccactc ccgcgcgaat
    12361 cgttgttgtg gccgaacgcg tcggtcgccg gctccccggc tccactcata gcggcacacc
    12421 cgcctgcaac gcaacggagg cgacggcgga cgaccgcagt tgacgctggc cccggggacg
    12481 gactaaccag atggttctgc gacgaggctc gaagcaacgg tgccgacaag gcatccagaa
    12541 tggcaaggga ggctgcaacg atgctcagca cattcgaccg gcgacacgca ctcaaggccc
    12601 tcggtgtcac cgcggcgggc gcggcgaccg gtttcccggc gccattcgtg cgcgcgcaag
    12661 gtgccaccat ccagatggtc agccaccgct atcccgcgct cgagcactat gcagcgaaga
    12721 tgcagtcggc gctgcccggg gtcgaggtaa acacccagct catgccgttc gacaaggcca
    12781 acgagctcgc gaccatcgcc ctctcgtccg gggcgagcac gttcgatatc gtctatgcca
    12841 acgacagcac agtgctgaaa tacgcgaaga atggctggct ccgcccgatc gacgacctgt
    12901 tcgagaaata caaggatgag ttcaattttg ccgactatcc ggagtcggtc ctcgacagct
    12961 atcgctacaa gggcgagctc ttcgtcctgc cgcacacagc caatgtcatg ctctttttct
    13021 atcgaaagga tctgttcgag gaggccggtg cggaggttcc gacgacgatg gaggaatacg
    13081 aggcgttggc cgcgcagttc aacagtccgg ctcgcgccgg caccatcagt tgccagaaac
    13141 cggtcgatgc gagtctcaac gaaatacact ggtacatgag cacgataggt gacgggtggt
    13201 tcgacgagaa cctccagccg gtgttcaaca gtccgaacgg tgtcgctgcg atcgagacgt
    13261 tgaagcggat caccgcaaat gcgcaacgcg gtttcacttc ggccgccaac gacgaatgca
    13321 cgatcgcgct gcagcaggat ctagccgtga tgggtctgca atgggcgacg cgctgcgcat
    13381 cgatggacga tcccgcgaaa tcacgtgttg tcggcctgat cgactgggcc gcggcacccg
    13441 gtggccacac ccggcttggc ggcgatggct acggcatttc tgcttatacc agccaggatc
    13501 cggatcagct cttccgcatc ctcgccattt cctccagcta tgagagcatg tcggaggctg
    13561 cgtcgatgat ggtgccaccg cgcagctcga ttctcaacga tccggcggta gccgagaagt
    13621 atcgtcattt tccgggcgtg ctcgccgcgc tggaaacggc tcagccgttt ccgtccctgc
    13681 cggaattcta tgagttgggt gagacgatca cacgccgcgt ccaccaggcg attaccgacg
    13741 agatggaggt gcaggcggca ctggatgccg cggctgcgga gacgataggg ctgttgcgcg
    13801 atcgaggtta cgacctctga gccgatcgag cgtcgtcatt cagccctgag cgactagaga
    13861 gccgtagtcg atgcatgccc agcggggttt gtggctgttc ctggtgccga gcggctccgt
    13921 tttgaccctg gttctgctct acccgctggg ctatgcgttc tatctcagcc tcttcaacta
    13981 ctatctgggc gccgagccca tcttcatcgg gctcggcaac tacatcgccc tgctccagga
    14041 cgaacggttc tggagctcgt tgcgcaccac cctcctcctc gtcggggcct cggtgtcgct
    14101 gcagttcgtg ctcggccttg ctgtcgcctt cggcctctac aagctgacct tcgccacgaa
    14161 ggccctgaac gtgctcctct tcctgccgca cgtcgtgacc ccggtggtcg ccgccctctt
    14221 cctgcgctgg atcttcatgg gccgcttcgg gctgatcgac gcgatcctca tcggcctgaa
    14281 catcttcccg cccgactggc tcggcagccc caactgggcg cggctggtcg tggtccttgc
    14341 cgagagctgg cagttcacgc ccttcatgat catcgtgctc tatgccgggc tcaacacggt
    14401 cgaccagagc caaatcgaat cggcgcgcat ggacggcgcc ggcaactgga tgctgctcac
    14461 ccgcgtcatg ctgccggcga tccgtccgct gatcctcttc gtgctcgcca tccggatcat
    14521 ggattccttc cgtttcttcg acacgatcta cgtgctcacc gcgggcggcc ccggcaccgc
    14581 gaccgagacc gtcaccatct acacctacgc cctgggcttc cgcctgctcg aggtcggcaa
    14641 ggcctcggcc ctcggtgtcc tcacgctgct gatcaccgcg accgccatcg cgctcgtcat
    14701 cgcctttctc taccgccgcg aaaagggtgc cttctgatga agcccagcct gcacacgcag
    14761 atgctgctct tccttgcgct ctgcgccttc accgcgatca acctgacgcc catcttctgg
    14821 gctttcatgg tctcgatcaa acagccggtc gacgccttcg ccgcaccgcc gatgctgatc
    14881 ttcacgccga ccttcgagtt ccactattcg gtatgggtcg agcgcgggtt ctggcgcttc
    14941 ctgctcaaca gcgtaatcat cgcggtggct gtcgtctgca tctccgtgcc gatcggcacc
    15001 atggcggcct atgccctttc gcgcatggaa ggggccaagg cccggggcat cctcttcggc
    15061 ctgctcgcca tgcggatgtt cccccacatc ctgctcgcca tccccttctt cgtcatcgcc
    15121 tcggcgatcg acctcatcga cacctatctg gccatggtgc tcgcgctcgt ggcgatcaac
    15181 cagcccttca ccgtctggct gatgcgcagc ttcttcctcg acgtgccgcg cgagctcgac
    15241 gaggcggcga ccatcgacgg cgccagcctc tggcagacct tcacccgcgt ggcgcttccc
    15301 gtcgtccggc cggggctctg ggtgacctcg ctcttcagcc tgctcctcgc ctacaacgag
    15361 ttcctcttcg ccctggtgct gaccggctcc agcaccaaga ccctgccggt ggcgatcgcc
    15421 gagtacgggg cggaggacat caactactgg tcgctctcgg ccgccgccgc gatcggcatc
    15481 atgctgccga tcgtgctctt catggcgttc atgcagcgcc acctcgtccg cggcctggcc
    15541 atgggtgccg tcaagggatg agcccgccat gaccgaagcc atcctcccgg caggcgccat
    15601 cgactgcgac gtccatcccg tcgtgccggg tatcgccgcc ctcgtcccct atctcgatgc
    15661 gtactggcag gagatgatcg acgtgcgcgg catcgagggc ttcgagagcc gcgcatatcc
    15721 gcccatggcg ccgatgagtg cccgcgcgga ctggcgcggg ccggacggtc gtgccgccga
    15781 gagcgtggag ccggtgcgcc gccagctctt cgatcagtgg ggcgagggcg gtgccattct
    15841 caactgcctc tatggcatcc agacgatcca cgatctctcg ctcgcggcag cgatggcccg
    15901 ggccgtcaac gactggctcg ccgccgaatg gctggccatc gagcccaggt tccgtgcgtc
    15961 catcgtcctg cctttgcaga gccccgagct cgcggtcgag gagatcgagc ggcgcgccgc
    16021 cgacaagcgc ttcgtccagg tcctcgccct cgtcatgggc gagcagccgc tcggccagcg
    16081 cgcgtactgg ccggtctggg cggccgccga gcgtcacggc ctgccgctcg cgatccacac
    16141 cggcagcgcc tatcgccggg cgatcaccgc cctcggctgg cccagctcct ggctcgagga
    16201 ttatgtcgac caggcccagg ccttccaggc gcaggtcgga agcctcgtca gccacggcgt
    16261 cttcaacagg ttcccgggcc tcaaggtggt cctgagcgaa tccggcatca cctggctgcc
    16321 ggccttcctc tggcgcttct ccaagaactg gcggggcctg cggatcgaga tcccctgggt
    16381 cgagcggccg ccgatcgaga tcgtgcgcga ccatttccgc ctgacgctgc agcccatcga
    16441 tgcacccgac ggggcgacct tcgagcgcat catcgatcag ctcggctcgg acgagatgct
    16501 gctcttctcc accgactacc cgcactggca gctcgacggc gaccagccca tgccggactg
    16561 gcttcctgcg ggcactcggc gtaaaatcgc tgtcgacaac ccgcgcgcga cctatccgcg
    16621 cctcggcgag gtgccatccg gaggcgatcg atgagcctgg acacagtgga cgacgttctg
    16681 gtcgagagca gaggcgaggc gcgcgccgag cggacgcggc cggtccgcag cacgatcgtc
    16741 gactgcgacg tccacccgac ggtgcgcacg gcgaaggatc tcgagcccta tctggcgcgg
    16801 cgctggtggg accacttcac cacctacggc ctgcggccga agcacggcaa cgccaccgcc
    16861 gatccctatc ccaaggcgca gccgcgcgcc gcgcgccgcg atgcctgggc gcccaacggc
    16921 gatcagccgg gcagcaatct cgccttcatg cgcgagcact atctcgaccc ctatggcgtc
    16981 gaatgcggca tcctgagccc gctcaacccg acgggccagg gcgaccagaa catcgatttc
    17041 tcgatcgcca tgacgcatgc cgtcaacgac tggcagctcg acgtcttcac ccggctggag
    17101 ccgcgtttca aggcctcgat cgtgatcccc tacgaggacg gcgaggcctc ggcgcgcgag
    17161 atcgagctgc gcgccggcac cgcagacttc gcccaggtgc tgttcctcac ccgcaccagc
    17221 gagccgatcg gcaagcgccg ctactggccg atcttcgagg cggcagcaca tcatggcgtg
    17281 cccgtggcac tccacgtctt cggctacagc ggccacccgg tcagcggcac cggctggccc
    17341 tcctactata tcgaggagat gaccggccat tcggccgcct gccaggctgc ggtcgccagc
    17401 ctcgtgctcg agggcgtgtt cgagcgcttt ccggagctga aggtggtgat catcgagggc
    17461 ggcttcggct ggctgccgtc cttgacctgg cgcctcgacc ggcactggca gaagctcggc
    17521 gccgaggtgc ccggttgccg gcgcccgcca tccgagtaca tccgtgagca tatctggatc
    17581 acgacgcagc cgatcgagga gccggaggac acggcggacc tgatccagac catggaatgg
    17641 atcggctggg accgcctgct cttcgccacc gactacccgc actgggactt cgacgacccg
    17701 atgcgcgccc tgccgcgcgg gctggatccg gcaaaccggc gcaagatcga ggccgagaac
    17761 gcccgagcgc tcttcaagct cggctgatgg cccggcacat cgtggcgacc gcccgggaga
    17821 ttcccgaagg cggcaggaag cgcgtcgtca tcaacagccg gccgatcgcc gtcttccact
    17881 cgaagggcga gtatttcgcg ctctacgaca cctgtccgca cgagggcggc agcctcagcg
    17941 ccggcaagct cacgggcctc gtccagtccg acggcccggg cgactaccgg ctctgccgca
    18001 agggcgagat cgtccgctgc ccctggcacg gctgggagtt cgatctgcgc accggccagt
    18061 cctactgcga cccgaggcgc atgcgggtga aaagctacga ggccggcacc acccgtccca
    18121 gcgaggccgt ggagggtccc tacaaggcca ccaccttccc ggtcacggtc gacgacgagt
    18181 acgtcttcgt cgaggtgtga gcgaattcag cccgcctgga ggcgctgcag ggcgagggcg
    18241 ccgaggaggg cgctgtcgtg gccgagttcg gctcgcgcca gctccacccg aaagagctcc
    18301 ggctcctcgg ccagcgctgt ccgcacccgg tcgagatagc cgtccgccaa gccgacgctg
    18361 ccgccgatgg ccacgcggtc gagacccagg atcgtggcga gatcggcgat cagggtggcg
    18421 acggccttgg ccgagccttc gatgatcgcc tcggcccagg cttgtccctg ggcggccgcg
    18481 gcgaagacgg cgcgcgcatc ggcctccgca tgaccctgcg ccgcggcctc ggccgcgatt
    18541 gcccggccgg cggcggtgct ttccacggta ccgtaccggc cgctgccgca tgcgtttcgc
    18601 ccgaacgggc tcgagacgaa gccgacatgg ccggccaggc cattctggct ttggatcaac
    18661 tgcccgccga gcacgatccc gccgccgact ccggtcgaga cggtgaggta agcgaaatgc
    18721 cggcagcccc ggccggcgcc gaaatgcgcc tcggcgaagg cggcggccgc ggcgtcattg
    18781 cagcaggtca cggcaccgac gatcctttcg agcgccgagg ccaggggaaa tccctggacg
    18841 gcctcgagga tgccgcgatt gacggcgtgc cagctttctt ctccgtcgat ccgcccggcg
    18901 acggcgacgc cgagcggtgc tcccggcgca tggccgagct tttgaagcag gccggcgatc
    18961 gtggcgagct gcgcatcggg ccccgccttg ccgtcggtcg cggtctgcag ccgctcctcg
    19021 acgatgcccc gcgcgacgcg agccgcggcg atcttggtgc cgccgaggtc ggcggcgaac
    19081 ccgtcgaggg attcgcgcag gagtctctgc atcaggcgcg ccccgccgcc ttcgcgacgg
    19141 cggcctcgaa ccagccagtg accagctcga gccgggtcag ggcggtgccg accgtgacgg
    19201 cgtcggcgcc ggcccggatg gccgaagccg cgagctccgg cgtgttcacc cggccttccg
    19261 ccatgacgaa gccgccgagc tcgcgaaagg cccggatcag ctcgagatcg acgccgtcgc
    19321 cccgtcccgc cgtttcggcg gtgtagcccg agagtgtggt gccgagcacc gcggcaccgg
    19381 cggcgagcgc ccgtcggccg tcttccgccg tggcgcagtc cgccatggcg agcgcgcgcc
    19441 cgtcccggat cgcctcgagg atagcttcgg ccgcatcggg gcgcggccgg atggtcgcgt
    19501 cataggcgac gatatcggcg ccggcggcga ccaggccccg ggcatccgcg atgagaggcg
    19561 tgatgcggac cgggctcacc tcgaggtcgc gcttgacgat gccgatgatc ggcacggtca
    19621 cgtgcggccg caccgcccgg aggttgtcga ttccctcgat tcggagaccc gcagccccgc
    19681 ccgccacggc ggccagcgcc atggcggcga cgatctccgg ccggtccatc ggcccatcgt
    19741 ccacaggctg gcaggagacg acgaggccgc cacgcagcgt gtcgagcaag ctcatggtca
    19801 ctccatcaag ccatcagccc gtcagtccat caggctgggc aggccggtcg agaggatcgg
    19861 gaaggcgcag accaggatga gcacgacgaa gggcgggatg agccagggca agatcgcccg
    19921 ggccagccgc tggtagggga tgttgccgac ctgggccacg acgaagagcg ccatgcccat
    19981 gggcggggtg aggatcgaga tcatcaggtt caagaccacg atgatgccga aatgcaccgg
    20041 atcgatgccg aagccggcgg cgatgggcac gaggatcggc acgatgatca agaggatcac
    20101 caaagcctcg atgaagcagc cgagcacgaa gatcatcaga ttgacggcga tcagaaaggt
    20161 gagcgggccg tcggcgatac cgaggaagag gctcgcgatc tgttgcggcg cctgctcgcg
    20221 cgccaggatg aagctgagga gggccacgcc ggcgacgatg agggcgatgg cggccgaggt
    20281 cacggtcgtc tcgtagatcg cgtcccacaa gcggcggaag gttagctcgc ggtagaagat
    20341 gacgtcgatg gcgatggcgt agagcacggt caccgccgcc gcctcggtcg gcgagaagag
    20401 gccggagaag atgccgccga cgatgaccac aggcgtcagg agcgccggca gcgaccggat
    20461 gaacaggctg ccaatctggc cgacgctctg gcgcggatcg cgcgggtagt cgcggcggcg
    20521 ggcgaggaca tagaccatca ccatgagcgc accggcgcag agcacgcccg ggatgatgcc
    20581 gccgaggaag agcttgccga tcgagacgtt ggcgatgacc ccgtagagca ccatggtgat
    20641 cgagggcggc accaaaggcc cgatcatcga cgaggcggcg gtgaccgagc ccgcgaagtc
    20701 gtcgtcgtag ccggcctcgc gcatcgcctc gatctccatc ttgccgagcc cgccggcatc
    20761 ggccaaagcc gagccggaca tgccggagaa gaagagcgag gccagcacgt tcacatggcc
    20821 gagcccgccg gtgacatgcc cgacgagtgc ccgggcgaag ccgaagagat ggttggtgat
    20881 gccggccgag ttgaggatgc cggccgccag gatgaagagc gggacggcga gcaggggaaa
    20941 gctgttcagc ccgtccacca tgcgctgcac ggcgaagctg atgcccatgc cggtccagta
    21001 gaagtagccc atggacacga agatcatcgc cacgccgatc ggcacgccga ccaggacgaa
    21061 gacgagccag agcgcaccga gagtggcgag tatcatagcg cagggctcct gtcggcggca
    21121 cggccattgc ggccgagcag gcgccgcagg atccgatgga tgatgaagaa ggacgcgacg
    21181 aaggcagaag cgtagagcaa ggcatcggac gtcggcaaag tgaccgagat gttgctccag
    21241 gtgcgctgga tggtgcgaaa gccgatccag cacaggtgcg ccatgatgcc cagatagacg
    21301 aggtcgatca ccgtgtagag ggcgcgccgg acggtggccg gcagggcatc ggcgaggaag
    21361 gccatcttca gttgcccatc ctggcgctcc acctcgccga tggcgatgaa agccatccag
    21421 acccagagcc accgcgccgc ctcctcggtc cagacggggc cgcgaaagag cggcgttcgg
    21481 ccgaagacct ggatcagcac gacgccgatc agggcgatga acagcacgga ggcgatgctg
    21541 ccctcgaggc tgtatttgcg tggctcggac aagacgctct cccctgcttc gacccggctg
    21601 cctggcggtg ggctggccgc gggcccggcg cccgcggccg cctcgatcag tcgccggtca
    21661 cggcggcgat cgagccttcg ccgaactgct cctcgagcgt gtcgtaatag ggctgcatcg
    21721 ccaggcggaa cggctccaga tccggccggg tgatggtcag gccacgctcc tcgaaggtgg
    21781 cgagcagatc cttctcggcc tgcatcgtca ggctgtcgtt gagctcgccg ccggcctcga
    21841 ccgccgccct gacccagccc tgctcctcct cgctcagatc ggcccaggtg tcggccgaca
    21901 tctgcaccgc cgccgaggcg acgaagtggt tggtcagggc gatgtgcgac tgcacctcgt
    21961 agaacttcat ggcgtcgatg gtcggcagcg ggttctcctc ggcgtcgacc tggttggtct
    22021 gcagcgccag atagacctcc tgaaaggcga ccggggccgg ggtggcgccg gtgttctcgg
    22081 cataggcgat caggaagggc acgttggggg tgcgcaggcg caggcccttc atgtc
//
LOCUS       DAOTFI010000047        22128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2794_length_22128_cov_7.677706, whole genome shotgun sequence.
ACCESSION   DAOTFI010000047 DAOTFI010000000
VERSION     DAOTFI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22128
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2794_length_22128_cov_7.677706"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /gene="sucB"
                     /locus_tag="P5558_06620"
     CDS             <1..699
                     /gene="sucB"
                     /locus_tag="P5558_06620"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRY24017.1"
                     /translation="REERVRMTRLRKRIAERLKEAQNTAAMLTTFNEVDMSAVIELRK
                     TYQDAFTKKHGIKLGFMSFFVKACIDALRVYPAANSEIDGDEVVYKHYYDIGVAVSSD
                     AGLVVPVVRGADAMNLAEIEAAIADLGSRAKSGKLAMEELTGGTFTITNGGIFGSMLS
                     TPILNPPQVGILGMHNIVQRPVVLPDGRIEARPIMYVALSYDHRIIDGREAVSFLVRV
                     KQCIEDPTRMLLDA"
     gene            complement(718..1071)
                     /locus_tag="P5558_06625"
     CDS             complement(718..1071)
                     /locus_tag="P5558_06625"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRY24018.1"
                     /translation="MSKRDPFVDHVLEMLEPLGMVRARAMFGGFGIFHVDRMIALIAN
                     ERLYFKTDQRTRPMFEAAGAEAFVYEGKRGKPSVMSYHEAPPECLDDAEAMLQWARLA
                     SEAALRGPVPKRTRS"
     gene            1432..2841
                     /gene="lpdA"
                     /locus_tag="P5558_06630"
     CDS             1432..2841
                     /gene="lpdA"
                     /locus_tag="P5558_06630"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRY24019.1"
                     /translation="MAEESFDVVFIGGGPGGYVGAIKAAQLGMKVACVEKRGALGGTC
                     LNIGCIPSKALLHSSHLYAEAKGGMEKHGIKIGSVELDLDAMMDNKDEVVETLTKGIE
                     GLFKKNKVTYVVGHGRIAGPGKVVVELNDGGERVLEAGAIVIATGSESTPLPGVEVDE
                     ERIVTSTGALDLDEVPAHLVVIGAGYIGLEMGTVWQRLGSKVTVVEFLDRVTPGMDGE
                     VSKQFQRILQKQGMSFKLGTKVTGVDASGDRLKVSVEPAKGGEGEVIDCDVVLVSIGR
                     RPYTEGLGLETVGLEPDRRGMIEVDDRFATKVAGIHAIGDVIRGPMLAHKAEEEGVAL
                     AEILAGQKPHIDYDRIPGVVYTDPEVAAIGKTEEQLKEAGIKYTVGKFPFMANSRARA
                     TGQTDGFVKMLADAETNRILGCHIIGADAGVMIGEIAVAMEFGASAEDVARTSHAHPT
                     LEEAIKEAALATYTKTLNL"
     gene            2921..3514
                     /locus_tag="P5558_06635"
     CDS             2921..3514
                     /locus_tag="P5558_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24020.1"
                     /translation="MSSLLRFLENGTAELARAFARSSDIQRLLAGACGADDYRRLLER
                     LRRGFAVIELQAFAGLPEPVHARLKTHRMTWRLRHDIGILGGTAPLPARAEDLENAGR
                     FSTASAKLGAQFALTLVLDSIARGQQALTACLGPALVSCLLFNGRRAAGEPQFAAVVR
                     SVIEDRADEIDWSEAMAAARRTLRTLRHALAETPRPA"
     gene            3651..4043
                     /locus_tag="P5558_06640"
     CDS             3651..4043
                     /locus_tag="P5558_06640"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRY24021.1"
                     /translation="MATPKTTPKFEMPKFEAPKFNFDAFVAMHKANVDTMVEAQGIYL
                     RTVEAMAKLQYGWVEETFKNGETLLKGDVTKRKPEDFLADAKSAAEKVMAVAKEQADL
                     GMKAQQEVADLVTKRVTANIEDAKSLAA"
     gene            complement(4142..5455)
                     /gene="deoA"
                     /locus_tag="P5558_06645"
     CDS             complement(4142..5455)
                     /gene="deoA"
                     /locus_tag="P5558_06645"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRY24022.1"
                     /translation="MSLPQEIIRKKRDREALGAEEIGTLVQGIVDGSLVDAQAGAFAM
                     AVVLNGMNRAETAALTRAMAGSGRRLTWPDLPGPAVDKHSSGGIGDKVSLVLAPMLAA
                     AGCFVPMVSGRGLGHTGGTLDKLESFRGYDTRPGLDRFARVVREVGCAIVGQTDDLAP
                     ADRRLYAIRDVTATVESEPLIVASILAKKLAVGTAGLLMDVKVGNGAFMTDLATARGL
                     ATAIAEVAAAAGLPTRSLLTDMNECLGRTAGNALEVGEAVAFLKGDARDPRLAEVTLA
                     LGAELLVLVDLAPDKKAARVVLEGCLDSGAAADRFARMVRALGGSGDPLHPSALAHAR
                     APVQLPVPSPAAGHVREIRTRELGLAVIGLGGGRTRPDQAIDPAVGLSGVLGTGRKVD
                     RGEPLALVHARDEAAGEAAVARIAEAYRIGDAPPALRPVVVESSP"
     gene            complement(5452..6222)
                     /gene="deoC"
                     /locus_tag="P5558_06650"
     CDS             complement(5452..6222)
                     /gene="deoC"
                     /locus_tag="P5558_06650"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRY24023.1"
                     /translation="MRLDLEQTLAAADARRLDPALARRLIGLIDLTSLTGDETPDAIT
                     DLARDAVDHGVAALCILPAALATAAPILAGSPVRLATVANFPHGGDDLARAADEVAAA
                     VALGADEVDVVAPLEAALAGDVGLAGELVELCRAAAGPETTLKLILETGRLERPELIT
                     AVGRSAVMAGIDFLKTSTGKTAVGATPEAVALLLRVAEEAGGRVGVKVSGGVRTAGEA
                     LRYLHLAETVMGPDWPDPAHFRLGASRLLADLAGRAAS"
     gene            complement(6212..7039)
                     /locus_tag="P5558_06655"
     CDS             complement(6212..7039)
                     /locus_tag="P5558_06655"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRY24024.1"
                     /translation="MPPTPTAAGYLKDRFGDGFPKIAVVLGSGLGGFADRLEAPTAVG
                     YDELEGFPRPSVEGHVGRLVVGTCGGVPVATLQGRVHVYEGQGTGTLATLVRALREVG
                     VRVLILTNASGAIAAAIRPGTIALITDHINMLGTNPLIGPNDQRHGPRFPDMSAVYDA
                     GLRGIVAASASSLRLEIASGVYLATTGPSFETPAEIRAFRALGADLVGMSTVPEAILA
                     RHAGLKVLGLSVVTNIAAGLRDAPLSHAETLAMGEAAAGDLERLLTASLPEIATHAA"
     gene            complement(7049..7432)
                     /gene="cdd"
                     /locus_tag="P5558_06660"
                     /pseudo
     CDS             complement(7049..7432)
                     /gene="cdd"
                     /locus_tag="P5558_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            complement(7429..8403)
                     /locus_tag="P5558_06665"
     CDS             complement(7429..8403)
                     /locus_tag="P5558_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24025.1"
                     /translation="MEELATQVLLLTASTVRLATPLVLCAMAGLFAERSGVVDIGLEG
                     KMLGAAFAAAAAAAVSGSAWVGLVAAIAVSWSLAMLHGFASITHKGDQVVSGMAINIA
                     VAGLGPVLADAWFRQAGRVPVSGEDARFLPIELPFAEAARSIPLIGPFYAEVVSDHNL
                     LVYVAAVSIPIVAWVVYRTRFGLRLRAVGENPSAVDTAGISVDGMRYRALMVTGLLAG
                     IAGAYLSIAQGAGFTRDMSAGRGYLALAALIFGKWRPVPAVLACLLFAFADAVQARLQ
                     GVELPLIGEIPTQAIQALPYLLTILLLAGFVGRAVAPRAIGQPYVKER"
     gene            complement(8407..9513)
                     /locus_tag="P5558_06670"
     CDS             complement(8407..9513)
                     /locus_tag="P5558_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24026.1"
                     /translation="MPTKLPPWVNFGLLPLLNLGMAFLVAGLIVVVLGENPFAAMRLL
                     VFGAFGYGEAVGYTFFYATNFIFTGLAVALAFQCGLFNIGGEGQAYIGGLGVGLAGIW
                     LGHWPAPIGLVVGIMAAAAFGGAWGFIPGWLQARRGSHTVITTIMLNFIAASLMTYLL
                     VNPLRRPGQQIPESMTFDERMWLPQLSTVLRDLGYDIGRSPLNAALLLALVCCVLYWL
                     FIWKTRAGYEMRVVGRSEPAAVYGGISPARQIMIAMTLSGALAGLMSLNEVLGAQHRL
                     TLGFTGGYGFVGIAVALMGRNHPIGIVLAALLFGALYQGGSELAFDMPKINKDMVVVI
                     QGLVILFAGALEHMFRPWLEARFARPALPAAAVA"
     gene            complement(9513..11060)
                     /locus_tag="P5558_06675"
     CDS             complement(9513..11060)
                     /locus_tag="P5558_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24027.1"
                     /translation="MSGAGGDGRTSGAPPAIELLGIDKSFGPVHANDDIHLTVEAGTI
                     HGIVGENGAGKSTLMNIVYGFYQADSGIIRVDGEERAIRSPEDAIEAGIGMVHQHFML
                     VEPFTVLENILLGAEGGWTLKAGKARAREALLRLEQDYALEVDIDARIADLPVGIQQR
                     VEILKALFRGARVLILDEPTGVLTPQEADHLFRILRNLKEQGRTVILITHKLREIMAA
                     TDTVSVMRQGAMVAHRRTADTTTEELAELMVGRKVLLRVDKQPARPGSPVLEVEHLEV
                     VDEQGVRRVKDVSFTVRAGEIVGIAGVAGNGQSELLEALSGIRRPSGGRVLLHGEPLA
                     DAADCRLKKLGHVPEDRLRMGLVRGFPAYENAILGYQLLPAYAEHGFLKKRSIIARCL
                     DWMQGYDVRPAAPHLQATSFSGGNQQKLVLAREMEHDPDLLLVGQPTRGVDIGAIEFI
                     HKRLVAMRDAGKAILLVSVELDEIMSLADRIIVMFDGTITGEVDQAEATARGLGLLMA
                     GISNEAA"
     gene            complement(11075..12073)
                     /locus_tag="P5558_06680"
     CDS             complement(11075..12073)
                     /locus_tag="P5558_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY24028.1"
                     /translation="MGRTGKSGFGLAMLALALMAGTAAAQDFKPAVIFDMGGKFDKSF
                     NEAAYNGAERFKEETGIEYLEFEVTQEAQREQAMRRMVRNGATIVVVVGFQNATPLET
                     VAPDNPDIEFTIIDAVVDQPNVQSVVFKEHEGSFLVGMAAAMASKTGKVGFVGGMDIP
                     LIRNFAHGYEQGVHYVNPDAEVLVNMTGTTPAAWNDPGKGGELAIGQFDQGVDVVYAA
                     AGATGLGVLQAAADRDKLSIGVDSNQNHLHPGSVLTSMLKRVDVAVYNAFKDSMDGTW
                     QPGAQSLGLAEDGVGFAIDENNRALITPEMEAAIEDAKAKIVAGEIVVEDYYSTQN"
     gene            complement(12164..13081)
                     /locus_tag="P5558_06685"
     CDS             complement(12164..13081)
                     /locus_tag="P5558_06685"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY24029.1"
                     /translation="MQDCRAAIALFCCTIVAACGSTLHPLPPAPRPAEVWATTEEPPR
                     AVILALHGFNDYRHAFADFATFAAARGYRVESFDQQGFGANPNRGLWPGSEALARDLA
                     RRVAELHRDWPGTPLYVLGESMGAAVAVVALGQGDLPPVDGVILVSPAVWGDTSFNGF
                     YRLVLEVAAGMAPGWTLTGRGLGVRASDNDEALIAMGRDPMLIKETRLDAIAGLVRLM
                     DEARQDAGKLDVPVLILLGERDEIIPPEAIRSFVGLVDPAVCHAIAYENGWHLLLRDL
                     QAETVWRDILAWIDGDTVAPAEWRCPKAG"
     gene            complement(13090..13959)
                     /gene="gluQRS"
                     /locus_tag="P5558_06690"
     CDS             complement(13090..13959)
                     /gene="gluQRS"
                     /locus_tag="P5558_06690"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRY24030.1"
                     /translation="MPGPGEQPLITRFAPSPTGYLHIGHAYSALFACEAARQSGGRFL
                     LRIEDIDSQRCRPEFERATYEDLDWVGIHWHGEPLRQSEHMSRYEGALLHLLEEGLLY
                     PCFCSRKQIRMEVEQSGRAPHGPSHELVYPGICRDIDPAERERRIVKGEPFAWRLDVA
                     RAMTRTGPLHWHDCRAGWVEARPEALGDPILARKDTPTSYHLAVVLDDHLQGVTLVSR
                     GEDLFHATHLHRLLQALFGITPPRYYHHNLIADSRGERLAKRNRAITLRHLRDIGRTP
                     ADIWKMLGLQQRV"
     gene            complement(14150..14785)
                     /locus_tag="P5558_06695"
     CDS             complement(14150..14785)
                     /locus_tag="P5558_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HRY24031.1"
                     /translation="MMSERITVDDLLLAHAAGRLPEPVGLVVATHLALAPAQRRVFRE
                     YEALGGALLEDLPPAELGEGALERLLDQLDDSIELAEPPPDGELPAPLRAYVKGSLAD
                     LPWRHFGGVSEARLPLDDSTYRTSLIRLKAGRAVPRHTHGGQELTVVLDGAFHDGIGA
                     YTVGDIAIADPHVDHQPQALGDEDCLCLAVTDAPLRLTGRFSRFLNPFLRF"
     gene            complement(14782..15561)
                     /locus_tag="P5558_06700"
     CDS             complement(14782..15561)
                     /locus_tag="P5558_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY24032.1"
                     /translation="MPPENSGRRLIRKPMRRVFPVQRMVRGTMASNFSGRLAAGPSGG
                     RDGLRLAWLASLRFKGAMRGSSSDADPSDAEPGGPDRELLADIRLVANDRDRTAFARL
                     HMYYAPRVKSYLRRIGSPDDVAEDLVQEVMIAVWRRAHQFDPARAALSTWVYTIARNK
                     RIDAFRRESHPDVDLTDPSMEPPPAPRGDVQVEAAVVKEGIRRAIEHLPPEQADLLRI
                     FYFEDKTHSAIADELGLPLGTVKSRLRLAVARLRGLLEGVA"
     gene            15629..16336
                     /locus_tag="P5558_06705"
     CDS             15629..16336
                     /locus_tag="P5558_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008854638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRY24033.1"
                     /translation="MSKTDHSELRQAIIATCLKMSELGINHGTSGNVSARIPEGLLIT
                     PSGMAYDSIRPADIVLIDHEGSYEGRRVPSTEWRFHYDIMRERPDINAIVHNHALHCT
                     TLACLRMEIPAFHYMIAVAGGDSVRVADYARFGTQELSDNALRALEGRTACLLANHGM
                     IALGADLGKALGLAVEIENLASCYWRCLQVTRPAILTTAQIDEVLVRIASYGKQPEEM
                     APGQMPATEAPPRRLAS"
     gene            complement(16356..17009)
                     /locus_tag="P5558_06710"
     CDS             complement(16356..17009)
                     /locus_tag="P5558_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY24034.1"
                     /translation="MPETVLRVLVLEDDPTDAFAMRRELAGRFEVTLAATLADALVRL
                     TTPGLKPDVIVTDLNLPDSNGPRTLEILRGASPDIPIVVSTGHLSEGVRRQLDLLGVA
                     HLHDKNDGFALLRAVLQHTESVHHSIAVHKAHISTEIERVARLAAEEAAGRAIGELVQ
                     RLGLHDEEGVRMAVRLARGWDAAKSRFAAAIAAGLASALLLAVSAGIVAILKNQGSK"
     gene            complement(17094..18338)
                     /locus_tag="P5558_06715"
     CDS             complement(17094..18338)
                     /locus_tag="P5558_06715"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRY24035.1"
                     /translation="MSEGPPPVRIDTIEAAAALLEGQVERTPLRHSRTLSDLTGAELW
                     LKFENLQYTASFKERGALNKLASLDPAERARGVVAMSAGNHAQGVAYHAARLGIRATI
                     VMPETTPFNKIRNTEQFGARVELRGETVDEAARHARELERDQGLVFIHPFDDPLIIAG
                     QGTIGLEIAADAPELDALVVPIGGGGLIAGIATALADRRPETEIVGVQSAAYPAHYNH
                     RLGKSPPLAAGRTIADGISVKEPGRLGRSIIDRLVSNIVLVDEPHLEQAILQLLEIEK
                     TVVEGAGAAGLAAVLADPERFRGRRIGVVLSGGNIDSRLLSAVIMRGLVRSERLIRLA
                     VMVPDSPGSLARLTRLIADAQGNVVEVTHKRAFSQGSVRDAIVDFVIETRDARHATQI
                     EAALAAAGFRVELAERAGPGFA"
     gene            complement(18340..19380)
                     /gene="argC"
                     /locus_tag="P5558_06720"
     CDS             complement(18340..19380)
                     /gene="argC"
                     /locus_tag="P5558_06720"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRY24036.1"
                     /translation="MSEIKVGILGASGYTGAELLRLLNDHPTAEARWLTADRNAGQPV
                     GSLFPHLAHLGLPDLQRLDDVAVDDVDVVFCCLPHGLTQKVVRELPETLRVIDLSADF
                     RLRDTALYAEVYGKPHEAVELQERAVYGLTEHYGEAIGKARLVANPGCYTTTAQLPLI
                     PLLKARLIDPTRIIIDAKSGVSGAGRAARESSLFTEISEGFHAYGVATHRHGPEIDQC
                     LSDFTERPVTVTFTPHLVPMNRGILATIYVRLQPDATVDGLQAALADAYRDAPFVHVL
                     PPRSLPATRHVRGSNNCLIAVHPSRVAGEAILLSVTDNLVKGASGQALQNMNLMLGLD
                     ETAGLRGAPLFP"
     gene            19460..20734
                     /gene="purD"
                     /locus_tag="P5558_06725"
     CDS             19460..20734
                     /gene="purD"
                     /locus_tag="P5558_06725"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRY24037.1"
                     /translation="MRFLVVGGGGREHALCWSLAASPLADAVLCAPGNAGIARDAECV
                     PVAVDDIDGLVALARAREIDLVVVGPELPLVLGLVDRLEALGIRAFGPSAAAARLEGS
                     KAFMKAFCARHGIPTAAHAVFTSDQAEAALAHVEAAPLPIVVKADGLAAGKGVVIAET
                     RAAARDAVEAAFAGAFGAAGATLVIEEFMAGEEASLFAICDGRTALEIGTAQDHKRAH
                     DGDQGPNTGGMGAYSPAPVLTPELIEEVLATIVRPTLDGLAAEGILYRGFLYAGLMLT
                     AAGPKLVEYNVRYGDPECQAVLPRLMTDLGQLVVGACDGILEHMDLRWYPDHALTVVL
                     AARGYPGPYARGSVIGGLEALEESDDVLLFHAATRREGERWLADGGRVLGITGLGATL
                     QEARDRAYAGVARLDWPEGFCRGDIGWRALPG"
     gene            20731..21705
                     /locus_tag="P5558_06730"
     CDS             20731..21705
                     /locus_tag="P5558_06730"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007282809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRY24038.1"
                     /translation="MTACRPADPAGIAHAARLLRQGGLVAFPTETVYGLGALATDDAA
                     IARVFEAKGRPAVNPLILHVATTAASREFAAFDDRAEALAAAFWPGPLTLILPLRQAA
                     AISPRAVAGLDTVAVRVPGHAVAQSLLAQVGGPVAAPSANRSGRVSPTTAAHVLADLD
                     GRIDLVLDGGPVQIGVESTVLDLSRPGRVGLLRPGGLTRAAIAAVTRPLDEPSSSPGE
                     PVRSPGLLLRHYAPRTALRLEATDVAPDEALLAFGPMPPTGAVATRNLSAKADLAEAA
                     HNLYAMLRELDAVGARRIAVMELPATGLGEALVDRLRRAAETPPDTAP"
     gene            complement(21713..>22128)
                     /locus_tag="P5558_06735"
     CDS             complement(21713..>22128)
                     /locus_tag="P5558_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRY24039.1"
                     /translation="GFAENQVAAAALLVEAALSDGDFGAAERQVIAGILGRHFDLPPN
                     LADRLLEKAEAAARDAVEWHGFTRAVKEATSHAERVGLIELLWEVALADEELHDYEAS
                     LIRRLCGLLYVSGRESAEARNRARERLDLPPPAAQ"
BASE COUNT         3419 a         7601 c         7885 g         3223 t
ORIGIN      
        1 cgcgaggagc gggtccgcat gacccggctc aggaagcgca tcgccgagcg gctgaaggag
       61 gcgcagaaca cggccgccat gctcaccacc ttcaacgagg tggacatgtc ggcggtcatc
      121 gagctgcgca agacctacca ggacgcgttc accaagaagc acggcatcaa gctcggcttc
      181 atgtcgttct tcgtgaaggc ctgcatcgac gcgctgcggg tctatccggc ggccaattcg
      241 gagatcgacg gcgacgaggt cgtctacaag cactactacg acatcggcgt ggcggtgagc
      301 tcggatgccg gcctcgtcgt gccggtggtg cgcggcgccg acgccatgaa cctcgccgag
      361 atcgaggccg cgatcgccga tctcggcagc cgggcgaaga gcggcaagct cgcgatggag
      421 gagctgaccg gcggcacctt cacgatcacc aatggcggca tcttcggctc gatgctgtcg
      481 acgccgatcc tcaacccgcc gcaggtcggc atcctcggca tgcacaacat cgtccagcgc
      541 ccggtggtcc tgccggacgg ccgcatcgaa gcccggccga tcatgtatgt cgccctctcc
      601 tacgaccacc ggatcatcga cggccgcgag gcggtgagct tcctcgtgcg cgtcaagcag
      661 tgcatcgagg accccacccg gatgctgctc gacgcttagc cggtcagccg ccggtcccta
      721 gctccgggtc cgcttgggca ccgggccgcg cagtgccgcc tcgctggcga ggcgggccca
      781 ttgcagcatg gcctcggcgt cgtcgaggca ttcgggcggg gcctcgtggt agctcatgac
      841 cgagggcttg ccgcgcttgc cttcgtagac gaacgcctcc gcaccggccg cctcgaacat
      901 cggacgggtg cgctggtcgg tcttgaagta gaggcgttcg ttggcgatca gcgcgatcat
      961 gcggtcgacg tgaaagatgc cgaagccgcc gaacatggcg cgggcgcgca ccatgccgag
     1021 cggctccagc atctccagca catggtcgac gaacgggtcg cgcttcgaca tcgattggaa
     1081 cctgggaacg ctttggagcc tgaaagaaga aaggccgccc ggaggcggcc tttccgtgag
     1141 cacggtggtc atccggccct ggaaatcaat ccgtggtggt caaaccagaa gcagaggctg
     1201 ctccgctgcc gatgccgacc acgtgggccc gaacgacgta tccgtcatct cactcgacat
     1261 tggatcacct cctttccgtt tgttgcgcct gggacgactt cgccaccgat ccgccgctgc
     1321 gtcaagtccc cacggaaggg tggggtttgt tcaaatgcct cgctccacta tgaagggcgc
     1381 ggttgcgagg tgctgcaggc gcctgtcgga ccggggcgaa gggggaaagc gatggcggaa
     1441 gagagcttcg acgtggtgtt catcggcggt ggcccgggcg gctatgtcgg cgccatcaag
     1501 gcggcccagc tcggcatgaa ggtcgcctgc gtcgagaagc ggggtgccct cggcggcacc
     1561 tgcctcaata tcggctgcat tccctcgaag gccctgctcc actcgtcgca tctctatgcc
     1621 gaggcgaagg gcgggatgga gaagcacggc atcaagatcg gctccgtcga gctcgatctc
     1681 gatgccatga tggacaacaa ggacgaggtg gtcgagaccc tgaccaaggg gatcgagggc
     1741 ctcttcaaga agaacaaggt cacgtatgtc gtcgggcatg gccggatcgc cgggcccggc
     1801 aaggtggtgg tcgagctgaa cgacggtggc gagcgggtgc tcgaggccgg ggccatcgtc
     1861 atcgccacgg gctccgagag cacgcccttg cccggcgtcg aggtcgacga ggagcggatc
     1921 gtcacctcca ccggtgccct ggatctcgac gaggtgccgg cgcacctcgt cgtcatcggc
     1981 gccggctata tcgggctcga gatgggcacg gtctggcagc gcctcggcag caaggtcacg
     2041 gtcgtcgagt ttctcgatcg ggtgacgccc ggcatggacg gcgaggtcag caagcagttc
     2101 cagcgcatcc tgcaaaagca gggcatgagc ttcaagctcg gcaccaaggt cacgggcgtc
     2161 gacgcctctg gcgaccgcct caaggtcagt gtcgagcccg ccaagggcgg cgagggcgag
     2221 gtcatcgatt gcgacgtggt gctcgtctcg atcggccgcc ggccctatac cgaaggcctc
     2281 ggcctggaga cggtcggcct cgagcccgac aggcgcggca tgatcgaggt ggacgaccgg
     2341 ttcgcgacga aggtcgcggg catccatgcg atcggcgacg tcatccgcgg gccgatgctg
     2401 gcgcacaagg ccgaggagga gggcgtcgcg ctcgccgaga tcctcgcggg gcagaagccg
     2461 catatcgact acgaccgcat tccgggcgtg gtctacacgg accccgaggt ggcggcgatc
     2521 ggcaagaccg aggagcagct caaggaggcc gggatcaagt acacggtcgg caagttcccg
     2581 ttcatggcca acagccgggc gcgcgccacc gggcagaccg acggcttcgt caagatgctg
     2641 gccgatgccg agaccaaccg gatcctcggc tgccacatca tcggcgccga tgccggggtg
     2701 atgatcggcg agatcgcggt ggccatggag ttcggcgcct cggccgagga cgtggcccgc
     2761 accagccacg cccatccgac gctcgaggag gcgatcaagg aggcggcgct ggcgacctac
     2821 accaagacgc tgaacctgta gcgggcagcg gtaaaggaat tattgcctat taaataggat
     2881 tattgtacct tccgggccga tcagaacccg ggagcctcca atgtcctcct tgctgcgttt
     2941 cctcgagaac ggcaccgccg agctggcgcg cgccttcgcc aggtcgtcgg acatccagcg
     3001 cctgctcgcc ggggcctgcg gggcggacga ttatcgccgc ctgctcgagc ggctgcgccg
     3061 gggcttcgcc gtcatcgagc tgcaggcctt cgcaggtctg cccgagcccg ttcatgcccg
     3121 cctgaagacc catcgcatga cctggcgcct gcgccacgat atcggcatcc tcggcggcac
     3181 ggcacccctg cccgcccggg ccgaggatct cgagaatgcc ggcaggttct ccaccgcctc
     3241 ggccaagctc ggcgcccagt tcgccctgac cctggtgctc gacagcatcg ccagggggca
     3301 gcaggcgctg accgcctgcc tggggccggc gctcgtctcc tgcctcctct tcaacgggcg
     3361 gcgcgcggcg ggcgagccgc agttcgccgc cgtggtccgt tcggtgatcg aggaccgggc
     3421 ggacgagatc gactggagcg aggccatggc cgccgcccgg cgcacgctgc gcacgttgcg
     3481 ccacgcgctg gccgaaacgc cgcgacccgc ctgaccgtcc gtttcctcgg accaggtgcg
     3541 acactttgcg ccaggtgttt atgctgcact gcacaaaagc gggcttgcga tttggcccct
     3601 cttgccccag ttattgtgca gcgcagcaaa ccgagagagg aaacagcgtg atggctactc
     3661 ccaagaccac cccgaagttc gagatgccca agttcgaggc tcccaagttc aatttcgacg
     3721 cgttcgtcgc gatgcacaag gccaatgtcg acaccatggt cgaggcccag ggcatctacc
     3781 tgcggaccgt cgaggcgatg gccaagctgc agtacggctg ggtcgaggag accttcaaga
     3841 acggcgagac cctgctcaag ggcgacgtga ccaagcgcaa gcccgaggat ttcctcgccg
     3901 acgccaagtc cgccgccgag aaggtgatgg ccgtggccaa ggagcaggcc gacctcggca
     3961 tgaaggcgca gcaggaggtg gccgacctgg tcaccaagcg cgtgaccgcc aacatcgagg
     4021 acgcgaagtc gctcgccgcc taagtcgcgg ctcgagcgtt ccgaggaagc cctccgcctc
     4081 cctggggtga actcggatct gcgtggaccc cgggcagcac gctgcccggg gtttcgcgcg
     4141 ttcagggact gctttcgacg acgacgggac gcaacgcggg cggcgcgtcg ccgatgcggt
     4201 aggcctcagc gatgcgcgcc accgccgctt cgccggccgc ctcgtcccgc gcgtgcacca
     4261 gcgccagggg ttcgccccga tcgacctttc ggccggtgcc cagcacgccg gagaggccga
     4321 cggccgggtc gatcgcctga tccggccgcg tgcgcccgcc accgagcccg atgaccgcga
     4381 ggccgagctc gcgggtgcgg atctcgcgca catggccggc tgccggcgac ggcaccggca
     4441 actgcaccgg tgcccgcgca tgggcgagcg cggacgggtg cagcgggtcg ccgctgccgc
     4501 cgagggcgcg gaccatccgg gcgaaccgat cggccgccgc cccgctgtcg aggcaaccct
     4561 ccagcaccac ccgcgccgcc ttcttatccg gcgccaggtc gacgaggacg agaagctcgg
     4621 caccgagcgc cagcgtcacc tcggcaagcc gggggtcgcg ggcgtcgccc ttcaggaagg
     4681 ccacggcttc ccccacctcg agcgcgttgc cggccgtccg gcccaggcac tcgttcatgt
     4741 ccgtcaggag gcttcgggtc ggcaggcccg ccgctgccgc cacctcggcg atggccgtgg
     4801 cgaggcccct tgcggtggcg aggtcggtca tgaaggcgcc attgccgacc ttgacgtcca
     4861 tgagcagccc ggccgtgccg acggcgagct tcttcgcgag gatggaggcg acgatcaagg
     4921 gctcgctctc gaccgtcgcc gtgacgtcgc ggatggcata gaggcggcgg tcggcggggg
     4981 cgagatcatc ggtctggccg acgatggcgc agccgacctc gcgcaccacg cgggcaaacc
     5041 ggtcgaggcc ggggcgggtg tcgtagcccc gaaagctctc gagcttgtcg agcgtgccgc
     5101 cggtgtgacc gaggccccgg ccggagacca tcggcacgaa gcagccggcg gcggccagca
     5161 tgggggccag gacgaggctg accttgtcgc cgatgccgcc cgaggaatgc ttgtcgacgg
     5221 cggggccggg cagatcgggc caggtgaggc gtcgccccga accggccatg gcccgggtca
     5281 gggccgccgt ctcggcgcgg ttcatgccat tcagcacgac tgccatggcg aaggcgccgg
     5341 cctgcgcgtc gaccagcgag ccgtcgacga tcccctgcac cagcgtcccg atctcctcgg
     5401 ccccgagcgc ctcgcggtcg cgtttcttcc tgatgatctc ctgcggcagg ctcaagaggc
     5461 ggcacgcccg gcgaggtcgg cgagaaggcg ggaggcgccg aggcggaaat gtgcaggatc
     5521 gggccagtcg gggcccatca ccgtctccgc gagatggagg tagcgaagcg cctccccggc
     5581 cgtgcgcacg ccgcccgaga ccttgacccc cacccgaccg ccggcttcct cggccaccct
     5641 gagcaggagg gccaccgcct ccggcgtggc gccgacggcg gtcttgccgg tcgaggtctt
     5701 gaggaagtcg atgccggcca tgacggcgga gcggccgacg gcggtgatca gctcgggacg
     5761 ctcgagccga cctgtctcga ggatcagctt gagcgtggtc tctggcccgg cagcggcccg
     5821 gcagagctcg accagctcgc ccgcgagtcc gacatcgccg gcgagcgccg cttcgagcgg
     5881 cgccaccacg tcgacctcgt cggcgccgag cgcgacggcc gccgccacct cgtccgccgc
     5941 ccgggcgaga tcgtcgccgc catgggggaa attggcgacc gtggcgagtc ggacggggct
     6001 gccggcgaga atgggtgccg ccgtcgccag cgccgccggc aggatgcaga gcgcggcgac
     6061 accgtggtcc acggcatccc gggccaggtc ggtgatggcg tccggcgtct cgtcgccggt
     6121 gaggctggtg aggtcaatga ggccgatgag ccggcgggcc agggccggat cgaggcggcg
     6181 ggcgtcggcc gcggcgaggg tctgttcgag gtcaagccgc atgggtggcg atctccggga
     6241 ggctggccgt gagcaggcgc tcgaggtcgc cggcggcagc ttcacccatg gccagcgtct
     6301 cggcatggct caagggcgca tcgcgcagac cggccgcgat gttggtgacg accgaaaggc
     6361 cgagcacctt caggcccgca tggcgggcga ggatggcctc gggcactgtc gacatgccga
     6421 ccagatcggc accgagcgcc cgaaacgcgc ggatctcggc cggcgtctcg aaggaggggc
     6481 cggtggtcgc gagatagacg cccgaggcga tctccagcct cagggagctg gcgctggcgg
     6541 cgacaatccc gcgaaggccc gcgtcgtaga ccgccgacat gtcgggaaag cgcggcccgt
     6601 gccgctggtc attggggccg atcagcggat tggtgccgag catgttgatg tggtcggtga
     6661 tgagcgcgat cgtgcccggc cggatcgcgg cggcgatggc gccgctggca ttggtgagga
     6721 tcagcacccg cacgccgacc tcccgcagcg cgcggaccag cgtggcgagc gtgccggtgc
     6781 cctggccttc gtagacatgg acgcgaccct gcagcgtggc gacgggcacg ccgccgcagg
     6841 tgccgacgac gagacggccg acgtggccct cgaccgaggg gcgggggaag ccctcgagct
     6901 cgtcatagcc gacggcggtc ggggcctcga gccggtcggc gaagccgccg aggccggagc
     6961 cgaggacgac ggcgatcttc gggaagccgt cgccgaagcg atccttcaga tagccggcgg
     7021 cggtgggcgt cggcgggatg ccagcgccga gggcgagcgg gccgaaggca taaggcagga
     7081 gctcccccat ggtggcggtc cgcctgccgc cgtcgctgcc cagcatgtgc accgccgtgt
     7141 cgccgggcgc gaactcgctc aggagctggc gacagccgcc gcagggggcg cagggcgcag
     7201 ctccattgcc gaccacgacg acctcggcga tgcgctgctc gcccgcggcc accatggcgg
     7261 ccacggccga ccgctcggcg cagaggccct gcgggtaggc ggcgttctcg acgttggcgc
     7321 cgacgaagac ggcgccgctg gccgtgcgga tggcgcagcc gacgaggaag cgcgaatagg
     7381 gggcataggc gcgctcgcgg acggcgcggg cggcggtcac gagatcggtc atcgttcctt
     7441 cacataaggc tggccgatcg ccctgggcgc cacggcgcgg ccgacgaagc cggcgagcag
     7501 caggatggtc aggagatagg ggagcgcctg gatcgcctgg gtcgggatct cgccgatcag
     7561 gggcagctcg acgccctgca gccgggcctg gacggcatcg gcgaaggcga agagcaggca
     7621 ggcgaggacg gcgggcacgg gccgccactt gccgaagatg agggcggcca gcgccagata
     7681 gccgcggccg gcgctcatgt cgcgggtgaa gccggcaccc tgggcgatcg agagataggc
     7741 gccggcgatg ccggcgagca ggccggtcac catcagggcg cggtagcgca tgccgtcgac
     7801 cgagatgccg gcggtgtcga cagcgctcgg gttctcgccg acggcccgca ggcgcaggcc
     7861 gaagcgcgtc ctgtagacca cccaggcgac gatcgggatg gagacggcgg cgacatagac
     7921 gaggaggtta tggtcgctca ccacctccgc atagaacggg ccgatcaacg ggatgcttcg
     7981 tgcggcctcg gcgaagggca gctcgatcgg caggaagcgg gcatcctcgc cgctcaccgg
     8041 cacccgcccc gcctggcgga accaggcatc ggcgagcacc ggcccgagcc cggcgacggc
     8101 gatgttgatg gccatgccgg agaccacctg gtcgcccttg tgggtgatgc tggcgaagcc
     8161 gtgcagcatg gcgagcgacc aggagacggc gatcgccgcc acgagcccga cccaggccga
     8221 gccgctgaca gcggccgccg ccgcggcggc gaaggcggcg cccagcatct tgccctcgag
     8281 gccgatgtcg accacgccgc tgcgctcggc gaagagcccg gccatcgcac agagcacgag
     8341 cggcgtggcc agccggacgg tgctggcggt caggagcagg acctgggtgg ccagctcctc
     8401 catcgctcag gcgaccgccg cggcgggcag ggccggccgg gcgaagcggg cctcgagcca
     8461 gggacggaac atgtgctcga gggcgccggc gaaaaggatg accagcccct ggatcacgac
     8521 caccatgtcc ttgttgatct tcggcatgtc gaaggcgagc tccgagccgc cctggtagag
     8581 ggcgccgaag agcagggcgg cgaggacgat gccgatcggg tggttgcgtc ccatcaaggc
     8641 gaccgcgatg ccgacgaagc cgtagccgcc ggtgaagccg agcgtcaggc gatgctgggc
     8701 gcccagcacc tcgttcagcg acatgaggcc ggcgagagcg cccgagagcg tcatggcgat
     8761 catgatctgc cgggccggcg agatgccgcc atagacggcg gccggctcgc tccggccgac
     8821 cacgcgcatc tcgtagccgg cgcgcgtctt ccagatgaag agccagtaga ggacgcagca
     8881 gacgagcgcc aggaggagcg ccgcgttcag cggcgagcgg ccgatatcgt agccgaggtc
     8941 gcggagcacg gtggagagct gcggcagcca catgcgctcg tcgaaggtca tgctctccgg
     9001 gatctgctgg cccgggcggc gcagcgggtt gaccaggaga taggtcatca gcgaggcggc
     9061 gatgaagttg agcatgatgg tcgtgatgac cgtgtggctg ccgcgccggg cctgcagcca
     9121 gcccgggatg aagccccagg cgccaccgaa cgccgccgcc gccatgatgc cgaccacgag
     9181 gccgatcggc gccggccagt ggccgagcca gatgccggcg aggccgaccc cgaggccgcc
     9241 gatataggcc tggccctcgc cgccgatatt gaagagcccg cactggaagg cgagggcgac
     9301 ggcgagcccg gtgaagatga agttggtcgc gtagaagaag gtgtagccga ccgcctcgcc
     9361 atagccgaag gcgccgaaga ccaggagccg catggccgcg aacggattct cgccgaggac
     9421 gaccacgatc agcccggcga cgaggaaggc catgccgagg ttcagcagcg gcaggagccc
     9481 gaaattgacc cagggtggca gcttcgtcgg catcaggcgg cctcgttgct gatgccggcc
     9541 atcagcaggc cgaggccgcg ggcggtggcc tcggcctggt cgacctcgcc ggtgatcgtg
     9601 ccgtcgaaca tgacgatgat ccggtccgcc agcgacatga tctcgtcgag ctcgaccgag
     9661 accaggagga tggccttgcc ggcgtcgcgc atggcgacca gccgcttgtg gatgaactcg
     9721 atggcgccga tgtcgacgcc ccgggtcggc tggccgacga gcaggaggtc cgggtcgtgc
     9781 tccatctcgc gggcgagcac gagcttctgc tggttgccgc cggagaacga ggtcgcctgg
     9841 agatgcgggg ctgccggccg gacgtcgtag ccctgcatcc agtccaggca tctggcgatg
     9901 atgctgcgct tcttgagaaa gccgtgctcg gcataggccg ggagaagctg atagcccagg
     9961 atcgcgttct cgtaggccgg gaagccgcgc acgaggccca tcctgagccg gtcctcgggc
    10021 acgtggccga gcttcttcag gcggcagtcg gcggcatcgg ccaggggctc gccgtgcagc
    10081 agcacgcgac caccactcgg ccgcctgatg ccggagagcg cttcgagcag ctccgactgg
    10141 ccgttgccgg cgaccccggc gatgcccacg atctcgccgg cgcggacggt gaagctcacg
    10201 tccttgaccc ggcgcacgcc ctgctcgtcg accacctcga gatgctccac ctcgagcacc
    10261 ggcgagccgg gccgggccgg ttgcttgtcg acccgaagga ggaccttgcg gccgaccatg
    10321 agctcggcca gctcctcggt ggtcgtgtcg gcggtgcgcc ggtgggccac catcgccccc
    10381 tggcgcatga cgctgaccgt gtcggtcgca gccatgatct cgcggagctt gtgggtgatc
    10441 aggatgacgg tccggccctg ctccttcagg ttgcgcagga tgcggaagag gtggtcggcc
    10501 tcctgcgggg tcagcacgcc ggtcggctcg tcgaggatca ggacacgggc gccgcggaag
    10561 agggccttga ggatctcgac ccgctgctgg atgccgaccg gcaggtcggc gatccgcgca
    10621 tcgatgtcga cctcgagcgc atagtcctgc tcgagccgga gcagcgcctc gcgcgccctg
    10681 gccttgccgg ccttgagcgt ccagccgcct tcggcgccga gcaggatgtt ctcgagcacg
    10741 gtgaacggct cgaccagcat gaagtgctgg tggaccatgc cgatgccggc ctcgatcgca
    10801 tcctcgggcg agcggatggc ccgctcctcg ccgtccaccc ggatgatgcc ggaatcggcc
    10861 tggtagaagc cgtagacgat gttcatcagc gtcgacttgc cggcgccgtt ctcgccgacg
    10921 atgccgtgga tggtgccggc ctcgaccgtc agatggatgt cgtcgttggc gtggaccggg
    10981 ccgaacgact tgtcgatgcc caagagctcg atggccggcg gcgccccgct cgtgcgcccg
    11041 tcgccgccgg ccccggacat gctgcctgct gccgtcagtt ctgggtcgag tagtagtcct
    11101 cgacgacgat ctcgccggcc acgatcttgg ccttggcgtc ctcgatcgcg gcttccatct
    11161 cgggcgtgat cagtgctcgg ttgttttcgt cgatggcgaa gccgacaccg tcctcggcga
    11221 gcccgagcga ctgggcaccc ggctgccagg tgccgtccat cgaatccttg aaggcattgt
    11281 agacggcaac gtcgacccgc ttcagcatcg aggtcagcac gctgcccgga tgcaggtggt
    11341 tctggttgct gtccacgccg atcgagagct tgtcgcggtc ggccgcggcc tgcagcacgc
    11401 cgagcccggt ggcacccgcg gcggcgtaga ccacgtccac gccctggtcg aactggccga
    11461 tcgccagctc gccgcccttg cccgggtcgt tccaggcggc cggcgtcgtg ccggtcatgt
    11521 tgacgagcac ctcggcatcg ggattcacgt agtggacgcc ctgctcgtag ccgtgggcga
    11581 agttgcggat cagcgggatg tccatgccgc cgacgaagcc caccttgccg gtctttgagg
    11641 ccatggcggc ggccatgccg acgaggaacg agccctcgtg ctccttgaag acgaccgact
    11701 ggacgttcgg ctgatcgacc accgcatcga tgatggtgaa ctcgatatcg gggttgtcgg
    11761 gggccaccgt ctcgagcggg gtcgcgttct ggaagccgac gaccacgacg atcgtggcgc
    11821 cgttgcggac catgcggcgc atcgcctgct cgcgctgcgc ctcctgggtg acctcgaact
    11881 cgagatactc gatgccggtc tcttccttga accgctcggc accattgtag gcggcctcgt
    11941 tgaacgactt gtcgaacttg ccgcccatgt cgaagatgac cgccggcttg aagtcctgcg
    12001 ccgcagccgt gccggccatc aatgccaaag ccagcattgc gagaccgaag ccgctcttcc
    12061 ccgtcctgcc catggtcatt gcctcaccct tcgggagatc ccctatttcg ggaaaaaccc
    12121 taggcgaggt gccggcaaat gcaagcaggt tcgctttggc ccctcagccg gctttcgggc
    12181 accgccattc cgccggggcg acggtatcgc cgtcgatcca ggcgaggatg tcccgccaga
    12241 cggtctcggc ctgcaggtcg cgcaggagga ggtgccagcc attctcgtag gcgatggcat
    12301 ggcagacggc cggatcgacg agcccgacga agctgcggat ggcttccggc ggaatgatct
    12361 cgtcgcgctc gcccagcagg atcagcacgg gcacgtcgag cttgccggca tcctgtcgcg
    12421 cctcgtccat cagccgcacc aggccggcga tggcgtcgag ccgcgtttcc ttgatgagca
    12481 tgggatcgcg acccatggcg atgagtgctt cgtcattgtc gctcgcccgg accccgagcc
    12541 cgcgcccggt cagggtccag cccggcgcca tgccggccgc gacctccagc accaggcgat
    12601 agaagccatt gaagctggtg tcgccccaga ccgccgggct caccaggatg acgccgtcga
    12661 ccggcggcag gtcaccctgc ccgagcgcca ccacggcgac cgccgccccc atgctctcgc
    12721 cgaggacgta gagcggcgtg cccggccagt cgcggtgcag ctcggcgacg cgccgggcca
    12781 ggtcccgggc cagcgcctcg ctgcccggcc agagcccgcg gttcgggttg gcgccgaagc
    12841 cctgctggtc gaaggactcg acgcggtagc ctcgggcggc ggcaaaggtg gcgaaatcgg
    12901 cgaaggcgtg gcgatagtcg ttgaagccgt gcagcgccag gatgacggcg cggggcggtt
    12961 cctccgtcgt ggcccagacc tcggcggggc ggggcgccgg cggcaacggg tgcagcgtgc
    13021 tgccgcaggc agcgacgatg gtgcagcaga agagggcgat cgcggcccgg cagtcctgca
    13081 aggtcggcgt tagacgcgct gctgcaggcc gagcatcttc cagatgtccg caggcgtgcg
    13141 gccgatatcc cgcagatggc gaagcgtgat ggcgcggttg cgcttggcga gacgctcgcc
    13201 ccggctgtcg gcgatcaggt tgtggtggta gtagcgcggc ggcgtgatgc cgaagagcgc
    13261 ctgcagcagc cgatgcagat gagtggcgtg gaagaggtcc tcgccgcggc tgaccagggt
    13321 gaccccctgc aggtgatcgt ccaggacgac ggccaggtga tagctggtcg gcgtgtcctt
    13381 gcgggcgagg atcgggtcgc ccagcgcttc cggccgcgcc tcgacccagc cggcccggca
    13441 gtcgtgccag tggagcgggc cggtgcgcgt catggcgcgg gcgacgtcga gacgccaggc
    13501 gaacggctcg cccttgacga ttcgccgctc gcgctcggcc ggatcgatgt cgcggcagat
    13561 gcccgggtag acgagctcgt gcgaagggcc atgcggcgct cgccccgact gctcgacctc
    13621 catgcggatc tgcttgcgtg agcagaagca cggatagagc agcccctcct ccagcagatg
    13681 cagcagggcg ccctcgtagc gggacatgtg ctccgactgc cgcaggggct cgccgtgcca
    13741 gtggatgccg acccagtcga ggtcctcgta ggtcgcccgc tcgaactccg gccggcagcg
    13801 ctggctgtcg atgtcctcga tgcgcaacag gaagcggcca ccgctctgcc gggcggcctc
    13861 gcaggcgaag agcgccgagt acgcatggcc gatatgcaga tagccggtcg ggctcggcgc
    13921 gaaccgggtg atcagcggct gctcgcccgg cccgggcatg ggcacggtgt cgatgccacg
    13981 caagacccgc tcctcgaggc atccgttcgc ggtcaaggtc acgcttcgcc catccgtcca
    14041 agctcggtcc cgatcacgcg catgttgcgg ataccggggc agcagtgcaa ggacgggaaa
    14101 cggcccttcc ggctcaaccg gaccgaacgt agccccggcg ccacaaacgt cagaaccgca
    14161 ggaacgggtt gaggaagcgc gagaagcggc cggtcagcct gagcggggca tcggtgacgg
    14221 cgaggcagag gcagtcctcg tcgccgagcg cctgcggctg atggtcgaca tgcgggtcgg
    14281 cgatcgcgat atcgcccacg gtataggcgc cgatcccgtc gtggaaggcg ccgtccagca
    14341 cgaccgtcag ctcctggccg ccatgcgtgt gcctcggcac cgcgcggcca gccttgagcc
    14401 ggatcaggct ggtgcggtag gtcgagtcgt cgaggggcag ccgggcctcg gacacgcccc
    14461 cgaaatgccg ccaggggagg tcggccagag atcccttgac ataggcgcgc agcggcgccg
    14521 gcagctcgcc atcgggcggc ggctcggcga gctcgatcga atcgtcgagc tggtccagga
    14581 ggcgctcgag ggcgccctcg ccgagctcgg ccggcggcag gtcctcgagc agcgccccgc
    14641 cgagcgcctc gtactcgcga aagacccggc gctgcgccgg cgccagcgcc agatgcgtgg
    14701 cgacgaccag cccgacgggc tccggcaggc ggcccgcggc atgcgccagc agcaggtcgt
    14761 cgacggtgat gcgctcgctc atcatgcgac accctcgagc agaccgcgca gcctggccac
    14821 ggccagccgc agtcgcgact tgacggttcc cagaggcagg cccagctcgt cggcgatggc
    14881 gctgtgggtc ttgtcctcga aatagaagat ccgcaggaga tcggcctgct cgggcggcag
    14941 atgctcgatg gcgcggcgaa tgccctcctt caccaccgcg gcctcgacct ggacatcgcc
    15001 gcgcggtgcg ggaggcggct ccatcgacgg atcggtcagg tcgacgtccg ggtgcgactc
    15061 gcgccggaat gcgtcgatcc gcttgttccg ggcgatggtg tagacccagg tgctgagcgc
    15121 cgcccgcgcc ggatcgaact gatgggcccg gcgccagacc gcgatcatca cctcctggac
    15181 gagatcctcc gccacatcgt caggggaccc gatgcgccgc agatagctct tcaccctggg
    15241 cgcgtagtac atgtggagcc gggcgaacgc cgtgcggtcg cgatcgtttg ccaccaggcg
    15301 gatatcagcg agaagctccc tgtcgggacc gccaggttcg gcatcgctcg gatcggcatc
    15361 gctcgaagag cctcgcatcg cacctttgaa ccgcagcgac gccagccatg caaggcgcag
    15421 accgtcgcgc ccgccgctcg gccctgccgc aagtctgccc gaaaaattgc tcgccatcgt
    15481 tccccgcacc atcctctgca caggaaatac gcggcgcatc ggtttgcgga tcagtcgccg
    15541 cccgctattc tccggcggca agatgcatcc ccaacggccc ggcgaacgcg ccccgaccct
    15601 gtcgggccta cgtgagtagc gatcaacgat gtccaagacc gaccacagcg agctccgcca
    15661 ggcgatcatc gccacctgcc ttaagatgtc cgagctcggc atcaatcacg gcacctccgg
    15721 caatgtgagt gcgcgcatac ccgagggcct gcttatcacc ccctcgggca tggcctacga
    15781 ttcgatccgg cccgcggaca tcgtcctgat cgaccatgag ggcagctacg aggggcggcg
    15841 cgtgccgtcg accgaatggc gcttccacta cgacatcatg cgcgagcggc cggacatcaa
    15901 cgccatcgtc cacaatcacg ccctgcactg caccaccctc gcctgcctgc ggatggagat
    15961 cccggccttc cactacatga tcgcggtcgc gggcggcgat tcggtccggg tggccgacta
    16021 tgcccgcttc ggcacgcagg agctctcgga caatgcgctg cgggcgctcg agggccggac
    16081 ggcctgcctg ctcgccaatc acgggatgat cgcgctcggc gccgatctcg gcaaggcgct
    16141 cggcctcgcc gtggagatcg agaatctcgc ctcgtgctac tggcgctgcc tgcaggtcac
    16201 ccggccggcg atcctcacca cggcacagat cgacgaggtg ctggtccgca tcgcttccta
    16261 tggcaagcag cccgaggaga tggcgccggg ccagatgccg gccaccgagg cgccaccgcg
    16321 ccggctcgcc tcctagatgc gggcggcagg ggctcttact tgctgccctg gttcttgagg
    16381 atcgccacga ttccggcact gaccgcgagc aggagggcgc tcgccaggcc cgcggcgatg
    16441 gcggccgcga agcgactctt cgcggcgtcc cagccgcggg cgaggcggac cgccatgcgc
    16501 acgccctcct cgtcgtgcag cccgagccgc tgcaccagct cgccgatcgc ccggccggcc
    16561 gcctcctcgg cggcgagccg ggccacccgc tcgatctcgg tcgagatgtg cgccttgtgc
    16621 acggcgatgg agtgatgcac ggattcggtg tgctggagca cggcgcgcag cagggcgaag
    16681 ccgtcgttct tgtcgtgcag gtgggcgacg cccaggagat cgagctggcg tcgcacgccc
    16741 tcgctcaggt ggcccgtgct gaccacgatc gggatgtcgg gggaggcccc gcgcaggatc
    16801 tccagggtgc ggggaccgtt cgaatcgggc aggttcagat cggtgacgat cacgtcgggc
    16861 ttgagcccgg gcgtcgtcag ccgcaccagg gcatcggcga gcgtggcggc cagcgtgacc
    16921 tcgaaacggc cggcaagctc gcggcgcatc gcgaatgcgt ccgtcggatc atcttcgaga
    16981 acgaggacgc gcaagaccgt ctccggcaca aaatcagcca ctcgaaatag gattaatacc
    17041 ctattatcgc ggcgcagtaa agccttgcgt gctctcccgc cgcagctctt cgatcaggcg
    17101 aagcccgggc cggcgcgctc cgccagctcc acccgaaagc cggccgcggc cagggcggcc
    17161 tcgatctggg tggcatggcg cgcgtcacgg gtctcgatga cgaagtcgac gatcgcatcg
    17221 cgcaccgagc cctgggagaa ggcgcgcttg tgggtcacct cgacgacatt gccctgggca
    17281 tcggcgatca gccgggtcag ccgggcgagg ctgcccgggc tgtccggcac catgacggcg
    17341 agccggatca gccgctcgct cctgacgagg ccacgcatga tcaccgcgct gagcagccgg
    17401 ctgtcgatat tgccgcccga gagcacgacg ccgatccggc ggccgcggaa ccgctccggg
    17461 tcggcgagca ccgcggcaag gccggcggca ccagccccct cgaccaccgt cttctcgatc
    17521 tcgaggagct ggaggatcgc ctgctcgaga tgcggctcgt cgaccagcac gatgttcgaa
    17581 acgagcctgt cgatgatgct ccggcccagc cggcccggct ccttgaccga gatgccgtcg
    17641 gcgatggtcc ggcccgccgc cagcggcggg ctcttcccca agcggtgatt gtaatgggcg
    17701 ggataggccg ctgactggac cccgacgatc tccgtttccg gccgacggtc ggccagcgcc
    17761 gtggcgatgc cggcgatcag gccgccgcca ccgatcggca cgaccagggc atcgagctct
    17821 ggcgcgtccg ccgcgatctc gaggccgatc gtgccttgcc ccgcaatgat cagaggatcg
    17881 tcgaagggat ggatgaagac gagcccctgg tcacgctcga gctcgcgcgc atggcgcgct
    17941 gcctcgtcga ccgtctcgcc ccgcagctcg accctggcgc cgaactgctc ggtgttgcgg
    18001 atcttgttga agggggtcgt ctccggcatg acgatggtgg cccggatgcc gagccgtgcg
    18061 gcatggtagg ccacgccctg cgcatggttg ccggccgaca tggccaccac gccccgcgcc
    18121 cgctcggcgg gatcgaggct ggcgagcttg ttgagggcac cccgttcctt gaacgaggcc
    18181 gtgtattgca ggttctcgaa cttcagccag agctcggcgc cggtgaggtc ggagagcgtg
    18241 cgcgagtggc gcagcggcgt gcgctcgacc tggccctcga gcagggccgc cgccgcctcg
    18301 atggtgtcga tccggaccgg cggcgggcct tcgctcatct cacgggaaga gcggcgcgcc
    18361 gcgcagcccg gccgtctcgt ccaggcccag catcaggttc atgttctgca gggcctggcc
    18421 cgaggcgccc ttgaccaggt tgtcggtgac gctgagcagg atggcctcgc ccgccacccg
    18481 gctcgggtga acggcgatca ggcagttgtt gctgccgcgc acatggcgcg tcgccggcag
    18541 ggacctcggc ggcaggacat gcacgaacgg cgcatcgcga taggcatccg ccagcgccgc
    18601 ctgcaggccg tccaccgtgg catccggctg cagccgcaca tagatcgtgg ccaggatccc
    18661 ccggttcatc ggcaccagat gcggcgtgaa ggtgaccgtg accggccgct cggtgaagtc
    18721 cgagaggcac tggtcgatct ccggcccgtg ccggtgcgtg gcgacgccat aggcgtggaa
    18781 gccctcgctg atctcggtga agaggctgga ctccctggcc gcccgcccgg caccgctgac
    18841 gccggacttg gcatcgatga tgatcctggt cgggtcgatc agccgcgcct tcaggagcgg
    18901 gatcagcggc agttgcgccg tcgtcgtgta gcagcccgga ttggcgacga ggcgggcctt
    18961 gccgatcgcc tcgccgtaat gctcggtgag cccgtagacg gcccgttcct gcagctcgac
    19021 cgcctcgtgt ggcttgccgt agacttcagc gtagagggcc gtgtcacgca ggcggaaatc
    19081 ggccgagaga tcgatgacgc gcagcgtctc gggcagctcg cgcaccactt tctgggtgag
    19141 gccgtgcggc aggcagcaga agaccacgtc gacgtcgtcg acggcgacgt catcgagccg
    19201 ctgcagatca ggcagcccca gatgggccag atgcggaaag agggagccga ccggctggcc
    19261 ggcattgcga tcggccgtga gccagcgcgc ctcggccgtc gggtggtcgt tgagcaggcg
    19321 caggagctcc gcccccgtat agccgctggc acccaggata ccgaccttga tctcgctcat
    19381 cgtgctcgcc atcaacactc gatctcacgt agcgaacggt ctaagcgatc gtgcctggac
    19441 gtgcaaagag tgagaggaca tgcgatttct cgttgtcggc ggcggcggcc gcgaacatgc
    19501 gctctgctgg tcgctcgccg cctcgcccct ggccgacgcg gtgctctgcg cgcctggcaa
    19561 tgccggcatc gcgcgggacg cggaatgcgt gccggtggcg gtggacgata tcgacggcct
    19621 cgtggcgctg gcccgggccc gcgagatcga cctcgtcgtg gtcgggccgg agctgccgct
    19681 ggtgctgggt ctcgtcgacc ggctcgaggc cctgggcatc cgtgccttcg gcccgagcgc
    19741 tgccgcggcc cggctcgaag gctcgaaggc gttcatgaag gccttctgcg cgcgccatgg
    19801 catcccgacg gccgcccatg cggtcttcac cagcgaccag gcggaagcgg cgctggcgca
    19861 tgtcgaggcg gcgccgctgc cgatcgtggt caaggcggac gggctcgccg ccggcaaggg
    19921 cgtggtcatc gccgagaccc gcgccgctgc ccgcgacgcc gtcgaggcgg ccttcgccgg
    19981 tgccttcggc gcggccgggg cgacccttgt catcgaggag ttcatggccg gcgaggaggc
    20041 cagcctgttc gccatctgcg acggcaggac ggccctcgag atcggcacgg cccaggacca
    20101 caagcgtgcc catgacggcg accaggggcc caataccggc ggcatgggcg cctattcgcc
    20161 ggccccggtg ctgacgcccg agctgatcga ggaggtgctg gcgaccatcg tccggccgac
    20221 gctcgatggc ctcgccgccg agggcatcct ctatcgtggc ttcctctatg ccgggctgat
    20281 gctgacagcc gccggtccca agctggtcga gtacaatgtc cgctatggcg atcccgaatg
    20341 ccaggccgtg ctgccgcggc tgatgaccga tctcggccag ctcgtcgtcg gcgcctgcga
    20401 cggcattctc gagcacatgg atctgcgctg gtaccccgat cacgccctca ccgtggtgct
    20461 ggcggcgcgc ggctatcccg gtccttacgc caggggctcg gtgatcggcg gcctcgaggc
    20521 gctcgaggag agcgacgatg tcctcctctt ccatgccgcc acgcgccggg aaggcgagcg
    20581 ctggctcgcc gatggcggcc gggtgctcgg catcaccggg ctcggcgcca cgctccagga
    20641 ggctcgcgac cgggcctatg ccggcgtcgc ccggctcgac tggccggaag ggttctgccg
    20701 gggcgatatc ggctggcggg cgctacccgg atgaccgcgt gccgccccgc cgacccggcc
    20761 ggcatcgccc acgcggcccg gctgctgcgc cagggcgggc tcgtcgcctt tccgaccgag
    20821 acggtctacg ggctcggcgc gctggcgacg gatgacgcgg ccatcgcccg cgtcttcgag
    20881 gcgaagggcc ggccggccgt caacccgctc atcctgcatg tcgcgaccac cgcggcgagc
    20941 cgggagttcg ctgccttcga cgaccgggcc gaagcgctcg ccgccgcctt ctggccggga
    21001 ccgctgacgc tgatcctgcc gcttcgccag gctgccgcga tctcgccacg ggcggtcgcc
    21061 gggctcgaca cggtggccgt tcgcgtgccg ggccatgctg tcgcgcaatc gctgctggcg
    21121 caggtcggcg gcccggtggc ggcacccagc gccaatcgct ccgggcgggt cagcccgacc
    21181 accgcggcgc atgtgctggc ggacctggac ggccgtatcg atctggtcct cgacggcgga
    21241 ccggtgcaga tcggtgtcga gagcacggtg ctcgacctga gccgcccggg ccgtgtcggc
    21301 ctcctgcggc cgggcgggct gacgcgggcg gcgatcgcgg ccgtgacccg gccgctcgat
    21361 gagccgtcct cctcgccggg cgagccggtg cgctcccccg gcctgctgct gcgccactac
    21421 gcgccgcgca cagccttgcg gctcgaagcg acggatgtgg caccggacga ggcgctgctc
    21481 gccttcggcc ccatgccgcc gaccggggcg gtggcgaccc gcaacctcag tgccaaggcc
    21541 gatctcgccg aggcggcgca caatctctat gccatgctgc gcgagctcga cgcggtcggc
    21601 gcccggcgga tcgcggtcat ggagctgccc gcgaccgggc tcggcgaggc gctcgtcgac
    21661 cggctgcggc gggccgccga gacaccgccg gacaccgcgc cctgaggcca gactattgtg
    21721 cggccggcgg tggcaggtcc aggcgctcgc gcgcccggtt gcgggcctcg gcgctctcgc
    21781 gaccgctgac atagagcagg ccgcagaggc ggcggatcaa cgacgcctcg tagtcgtgca
    21841 gctcctcgtc ggcgagcgcc acctcccaga gcagctcgat caggccgacc cgctccgcgt
    21901 ggctggtcgc ctccttcacc gcccgggtga agccgtgcca ttcgaccgca tcgcgggcgg
    21961 ccgcttcggc tttctcgagc aaccgatcgg ccagatttgg cggcaagtcg aaatgccggc
    22021 cgagaatgcc ggcgatgacc tgccgctcgg ccgcaccgaa atcgccgtcg ctcagcgccg
    22081 cctccacgag gagcgctgcg gccgccacct ggttctcggc gaagcccg
//
LOCUS       DAOTFI010000048        22017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2815_length_22017_cov_7.482834, whole genome shotgun sequence.
ACCESSION   DAOTFI010000048 DAOTFI010000000
VERSION     DAOTFI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 22017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22017
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2815_length_22017_cov_7.482834"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="P5558_06740"
     CDS             complement(<1..325)
                     /locus_tag="P5558_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRY24040.1"
                     /translation="MRFRKRQRLVLIGLALLLLGGATALTLTALGDKIAFFVTPTQIV
                     GGEVAVDRNFRVGGLVVDGSIRRESDGSVAFRLTDTANEIEVHYQGILPDLFREGQGI
                     VAQGTL"
     gene            complement(332..562)
                     /gene="ccmD"
                     /locus_tag="P5558_06745"
     CDS             complement(332..562)
                     /gene="ccmD"
                     /locus_tag="P5558_06745"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HRY24041.1"
                     /translation="MEFIEMGGYGGYVWSAFGFTLVLTLGLAWQSWRQQRQRGTELME
                     LRRRVRSGDAGRPVRRLVAVRADGQPGPSSGS"
     gene            736..3510
                     /locus_tag="P5558_06750"
     CDS             736..3510
                     /locus_tag="P5558_06750"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF003467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRY24042.1"
                     /translation="MSLLAPDLQPRPELTCRQQIGAPLPEDEQAESATAVLARMRELL
                     GESERRLEAGFAEEGDGERVVRERARLLDGLLAGALDFAESQLFRVANPTRGEEMALL
                     AVGGYGRGELAPHSDVDVLFLYPYKPTPHLEQMTEFLLYRLWDLGLKVGQATRSLNEC
                     IKLAKSDLTIRTTLLETRLLWGSQRLTRELEGRFRAEVCKGHEPEFIEAKLAERDARH
                     RRTGDSRYLLEPNIKDGKGGLRDLHTLIWLARFIYDIADWDDLVRHGVLSRAELNGFL
                     RSRRFLWTVRCHLHYLTGRAEERLTFDLQPEIARRMGYRDRPRTLGVERFMKSYYLVA
                     KEVGSLTRLVCAALEEQHKRRPRFALPRFGPSQRRIGGFVVQGSRLALADPKLFQTRP
                     IAMLELFHTAQARELDIHPMALTALAQNLRRVDRQLCQSPEANRLFIEMLTSRKDPAQ
                     TLTRLNEAGVLGRLVPDFGRVVAQMQHNLYHVFTVDEHTIRVIDNLHRIEEGTLEADH
                     PLSTRILPQLTSRTELYVAAFLHDVGKGRGGDHSEVGARIAERLGPRLGLGQAGTETV
                     AWLVRHHLVLSNFAFKRDSEDPKTLQDLVAIVQSPERLRLLLVLTVADIRAVGPGVWN
                     GWKGQLLRELYHEAEAAMASGDLHGRRRERIERARASLAEALLAGGDGDRWTSAEVET
                     YTALHDPRYWLGVAEDAHLRHARLVRRAQTQKLPLAVDFHVDEFRARTEITVYAADHP
                     GLFMKVAGALALSGVSIVDAHIFTTADGMALDTLGVQDAERQAAVDDEARFGRIERNI
                     RKVLQGEVWLEKELAGRRSLPPRTEVFTVEPRVLVNNQASSTHSVIEVNGRDRPGLLF
                     ALAKVLKERGLVIHSAHISTYGERVVDVFYVKDVFGLKVASPAKLKRLRNELEAALAE
                     PADRASGKSG"
     gene            3518..5068
                     /gene="murJ"
                     /locus_tag="P5558_06755"
     CDS             3518..5068
                     /gene="murJ"
                     /locus_tag="P5558_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRY24043.1"
                     /translation="MNIVKATLTVGSFTAISRVLGFVRDLLIAAVLGAGAAADAFFVS
                     FKLANLLRRLFAEGAFNAAFVPLFARALEGEGEEEARRFAEEALAVMTAVLLVVVLVA
                     EIAMPWLVRGLAPGFEPAGERYGLAVELSRITFPYLMLISLAALFSGVLNSLGRFAAA
                     AIAPIILNVVLITTLLLASLHPATPAHALAWGVSVAGVLQLAWVAVAARRNGMGLKLR
                     RPRMTPRLKRLFQLMLPGAIGSGVYQINLIVDTWFASLLPVGAVSYLFYADRLNQLPL
                     GIIGVAIGTALLPMLSRQLRVSDFANASRTQNRAIEIGLLFTLPAAAGLIVLAEPIIA
                     VLFERGAFGPAETAATAGALVAFSIGLPAYVLIKVLAPGFFAREDTATPVKVAALCLV
                     ANVLLILLLIGPLRHVGIALATALSNCLNVACLAWILQRRGHLHIDERLRRIVPRIVV
                     ATVAMALILVAARLGLDFLPAALRLMAAIASGGLCFILACHWTGAADFAELRRMVSRR
                     RPAAEPGA"
     gene            5131..6132
                     /gene="trpS"
                     /locus_tag="P5558_06760"
     CDS             5131..6132
                     /gene="trpS"
                     /locus_tag="P5558_06760"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRY24044.1"
                     /translation="MPSTSRNRIFSGIQPTHGIQLGNYLGAIRNWVKQQDQFECIYCI
                     VDQHALTTVQNADEMRRNTREMGAALIASGIDPARSVLFVQSRVEAHSRLAWVLNCVT
                     PLGWVNRMTQFKEKAGKQRENAPVGLFVYPVLQAADILAYRATHVPVGEDQKQHLELS
                     RDIAASFNRRFAEDFFPLPEPMIQAEAARVMSLRDGTKKMSKSDPSDMSRINLTDDAD
                     TIASKIRKAKSDSILGISYDPENRPEASNLLTIFAALSDRERADVAAEYESASFASFK
                     EHLAELCVEKLAPINAEMRGLLANPDHIDAVLGDGSARADALARPIVDAAYEAVGFR"
     gene            6259..6816
                     /locus_tag="P5558_06765"
     CDS             6259..6816
                     /locus_tag="P5558_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRY24045.1"
                     /translation="MFIQTEVTPNPATLKFLPGRPVMTEGVVDFTEAAKAEAVSPLAA
                     RLFAVDGVAGVYLGADFISVTKNSADDWYMMKPAVLGAIMEHFMSDDPVLAAGADIDD
                     DVKVDEADATVKQIKELIDTRVRPAVAQDGGDIVFQGFERGIVYLHMRGACSGCPSSV
                     VTLKNGIENLLKYYVPEVVEVRSVS"
     gene            6833..7510
                     /gene="tsaB"
                     /locus_tag="P5558_06770"
     CDS             6833..7510
                     /gene="tsaB"
                     /locus_tag="P5558_06770"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRY24046.1"
                     /translation="MRLLAFDTAGDGCSAGCFAGAEPVAFARHAGASGQAERLVPMLA
                     AVLADAGWGMEDLEALAVTIGPGSFTGIRTGLAAARGLALVRRLPAWPVTTLAALAAV
                     ADGADGLPLAVVLDARRGQVFLQRFDSSGAALDEPLMLAPELAAARLREPHHLVGSGA
                     VALLRHLDATAMTLGDAVLDAVAVARAAAAARGRGEAPVHGTALAPLYLREPDARPSA
                     GRSLLQA"
     gene            7514..8044
                     /locus_tag="P5558_06775"
     CDS             7514..8044
                     /locus_tag="P5558_06775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY24047.1"
                     /translation="MLSFHQEPRLTIKPVGAFDLGRLARLHRHCFEEGWSRSDLAHLL
                     ALPGGFGLIARRGGTFVGLDALRGVGFALLRVVRDECELLSLGVLPAHRRRGVAGELL
                     RTAMQSCLDGGAQSMFLEVAIDNLSAQALYDRFGFVRVGTRPDYYARPDGTRMHAYTM
                     RCDLVAALGSRAPQPR"
     gene            complement(8060..8293)
                     /locus_tag="P5558_06780"
     CDS             complement(8060..8293)
                     /locus_tag="P5558_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRY24048.1"
                     /translation="MKADAELDISGETCPMTFVRTKIRLEAMRPGELLRVRLRGGEPL
                     ANVPRAVLDHGHSIIVEEAIADDLWELVIRVAE"
     gene            8431..8862
                     /locus_tag="P5558_06785"
     CDS             8431..8862
                     /locus_tag="P5558_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HRY24049.1"
                     /translation="MSDQLSQNELLSLTTEIVASHVSNNTVAVSDLPQLIRLVYTGLA
                     EQGQTKAEEEPEKLVPAVPIKKSIQPDHLICLEDGKKLKMLKRHLKTRYDMTPDEYRR
                     RWGLTDDYPMVAPEYAEQRSSLAKKIGLGTKPRARGRKAAS"
     gene            9222..10655
                     /gene="miaB"
                     /locus_tag="P5558_06790"
     CDS             9222..10655
                     /gene="miaB"
                     /locus_tag="P5558_06790"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRY24050.1"
                     /translation="MTFGQTAPDTGKAAPKKLFIKTYGCQMNVYDSDRLAALLARHGY
                     ATVPEAADADLVVLNTCHIREKAAEKVYSELGRLRVMRDQRRSETGRPMALVVAGCVA
                     QAEGEAMLGRAPFVDIVVGPQAYHRLPELLARSTREAGGQIDTDFPLESKFDLLPQGP
                     EESPVSAFLTVQEGCDKFCTFCVVPYTRGAESSRPAAAIVAEARRLVEGGARELTLLG
                     QNVNAYHGDGPDGRSWSLARLLHELAGIERLERLRYTTSHPRDMTADLIEAHGALPQL
                     MPFLHLPVQSGADRVLQAMNRGYGRDDYLRIVDALRDVRPDIALSSDFITGFPGETEE
                     DFEATLALVERVGFAQAFSFKYSPRPGTPGAAMTKQVKEAVRGERLNRLQALLDAQAT
                     AFNEGTLGRTLPVLLERPGRHPGQLVGRSPYLQAVHVAAPDGLLGRVARVTITGCHRH
                     SLAGRMEPHGPITASNGIPAQPEEVVA"
     gene            10730..11635
                     /locus_tag="P5558_06795"
     CDS             10730..11635
                     /locus_tag="P5558_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRY24051.1"
                     /translation="MLFGRHHMNIARLEQRLPVSIVTRGNEIFIQGESDADVRTAATV
                     LERLYEQLQGGHSIDREDVDAALRMVQGDVVDAGFVREVRTDRRSIMPRSPNQAAYLR
                     LLEQRDLVFALGPAGTGKTYLAVAAAVSFMRQRRVERIILSRPAVEAGERLGFLPGDL
                     KEKVDPYLRPLYDALQDMLPDGKLQTRIEQGEIEIAPLAFMRGRTLSHAFVILDEAQN
                     TTPAQMKMFLTRLGEGSRMVVTGDPSQIDLPAGAPSGLADAAARLQGIQEIGMVVFDK
                     SDVVRHPLVTRIVEAYEDEQPAAAG"
     gene            11735..12295
                     /gene="ybeY"
                     /locus_tag="P5558_06800"
     CDS             11735..12295
                     /gene="ybeY"
                     /locus_tag="P5558_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRY24052.1"
                     /translation="MDSDSSPSIEVAVGNGAWRSAVTDPEVICHQAATAALRAAAETY
                     FSTGVPDRLEVSIRLTDDAEVQALNRTWRGQDKPTNVLSFEGDDPGLVDPRTPSDAPW
                     LLGDVVLACETVTREAEADGRPVAAHLAHLVVHGVLHLLGHDHEDEVDALTMEGLESR
                     IVEGLGYADPHGEPGLQPAPLAEARP"
     gene            12292..13224
                     /locus_tag="P5558_06805"
     CDS             12292..13224
                     /locus_tag="P5558_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRY24053.1"
                     /translation="MRQLARRQDTSPSMLEGLLRRLRELASGESSTGGLRETLEDLLE
                     EEDSGPGAEQFTPEERELVLNALSFGELRVDDVMVPRADIRAIEINTGLDDVVAAMRE
                     AGHSRLLVFRDRLDDVVGLVHIKDLLPFWGDGGDFALSAVMRQVPVVPPSMRVLDLLL
                     EMRKSHAHMVAVVDEFGGTDGLVTVEDLVVEILGEIHDEHAPDEPPQLVHRADGSIEA
                     DGRVDLEDLEEQLGQTLLDGDERDEVDTLGGLIFTLVDRVPDIGESVHHPVGLTFHIL
                     DADPRRIKRVRIEIVDPEERQPRRLAGELLGPGD"
     gene            13239..14819
                     /gene="lnt"
                     /locus_tag="P5558_06810"
     CDS             13239..14819
                     /gene="lnt"
                     /locus_tag="P5558_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRY24054.1"
                     /translation="MAGRVPAAAAERPARLWLTPIAGAALALALPPLGFLPAALALGL
                     LFVQLQGARGYWPAFARAWLFGFGFHLAGLYWVGIAFLAEADRFGALAVPGVVALAAI
                     LAVIGAMPLALLGPLRLRQPIAAAAVFAGLWCLGELARGQHGVQFPWNPLALALAATD
                     TSLQVVALVGTTAGGFLVAWLAALVGVALTVRAARRTALALCLAMVAGLAAFGLWRLA
                     SHPPGLMAEPPVTVRIVQANIAQHHKWDPALRRNWFDRHLDLARQPIRPDAPDRPDIV
                     VWPESSVPYSLDAEPEVRGLIGDVVRPGGVVILGSNHFDGSVEPPILHNSVYALAGDG
                     ELLGRYDKVDLVPFGEFLPMRPLLGAVGLEALAVGSMDFQAGPGRTTMALPGLPPASP
                     LVCFEAAFPGRGTDGSGRARWIVNVTNDAWFGISSGPYQHAAMARMRAVETGLPLVRA
                     ANTGISMLTDPMGRVLDSLPLGTTGTIDASLPAALPTSPPITRWPWLSSLLMGLALLA
                     AGAAELAGRRSSPRDRDH"
     gene            14970..16136
                     /gene="metK"
                     /locus_tag="P5558_06815"
     CDS             14970..16136
                     /gene="metK"
                     /locus_tag="P5558_06815"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRY24055.1"
                     /translation="MVANDYLFTSESVSEGHPDKVCDRISDEVVDAYLAAEPEARVAC
                     ETLTTTNRIVIAGEVRGPAGLTHDHVRCIAREAVRAIGYEQDGFHWRDAAVDVYLHEQ
                     SSDIAMGVDAGADKEEGAGDQGIMFGFACDETPDLMPAPILYAHQILRLMAEARHAGQ
                     TPELAPDAKSQVTLRYAGGRPIACTSLVVSTQHKASAGREQVREIVRGFAKQVLPEGW
                     MPAEDEFYVNPTGQFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRS
                     AAYAARYLAKNVVAGGLARRCLIQLSYAIGIAKPLSIYVDTQGTGEVDEARLADLMMD
                     LDLSPRGIRRRLQLNRPIYARTSAYGHFGRQPTADGGFSWEQTDLADELKRAFN"
     gene            16075..16866
                     /gene="trmB"
                     /locus_tag="P5558_06820"
     CDS             16075..16866
                     /gene="trmB"
                     /locus_tag="P5558_06820"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRY24056.1"
                     /translation="MAASPGSRPISPTSSSAPSTDPTAEPPRRQRVLYGRRSGPGLRP
                     GQRERLDRLLPRLAIDLPAAGALDPASLFTPPRQRVWLEIGFGGGEHLAAQAAANPTV
                     GLIGVEPFVDGVARLLAQVEERQLHNVRLLVDDARLLLDRLPEASIERLFVLFADPWP
                     KKRHWKRRIVNAETVARFADLLVDDGELRVATDDPGYRRWMLACLLGEQRLDWCVRGP
                     EDWRDRPPDWPPTRYEKKALAAGRRPVFLRFRRKSRTAELATSAE"
     gene            17008..17535
                     /gene="rimP"
                     /locus_tag="P5558_06825"
     CDS             17008..17535
                     /gene="rimP"
                     /locus_tag="P5558_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRY24057.1"
                     /translation="MTTAHEGSRIEPLAALLAPTLHDLGFDLVQVRLIGGQRRTLQVM
                     AEPLDRGVTMTVEHCADISQAVSAVLDVADPIAGAYVLEVSSPGLDRPLVRLDDFARF
                     AGDEVRLEVEPPLDGRKRWRGRLEGVAGEDVLLTVDGLTRRVPYPTIRKAKLVLTDAL
                     VAAALKGGAGQEARE"
     gene            17539..19125
                     /gene="nusA"
                     /locus_tag="P5558_06830"
     CDS             17539..19125
                     /gene="nusA"
                     /locus_tag="P5558_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRY24058.1"
                     /translation="MELSTALGRAELLRVAETVAQEKGIDTAEVIDALEQAVQTAARR
                     KYGLEHDIIAEIDRKTGEIRLFRQLEVAEVVEDYAKQLSLDEARDRNPAAQPGDLILD
                     ALPPIDLGRIAAQSAKQVIVQKVRDAERERQFDEFKDRIGEIVIGEVKRTEHGNVLVD
                     LGRAEAIVRRDDLIPRESFRNKDRIRAYLYDVRHETRGPQIFLSRTHPQFMAKLFEQE
                     VPEIYDSIIEIKSVARDPGSRAKIAVISKDSSIDPVGACVGMRGSRVQAVVQELAGEK
                     VDIIPWSPDPATFVVNALAPAEVSKVVLDPEANRIEVIVPDHMQHIAIGRRGQNVRLA
                     SQLTGWDIDIVSEGEDSERRNREFREATELFIESLNVEEVIAQLLATEGFANVEDVAY
                     SETDELAAIEGFDEEIAEALKERAAAFLEEREQEMNAEADELGIGEDLRAFTLIDLKT
                     VVALGRQGVKSLEDFADLATDELVEMLAAEAPIDEVTAADLILQARLVLGWIEPEPEP
                     EELLEAADATPDDGTAETTH"
     gene            19131..19811
                     /locus_tag="P5558_06835"
     CDS             19131..19811
                     /locus_tag="P5558_06835"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY24059.1"
                     /translation="MLATAPTDSPTGGALPAPPRRRDIVSRESGDRSRMLRFVVDPER
                     RLVPDLAARLPGRGIWVIADRESLAQAVKKRLFDRQAGGPVVVPEDLGGLVERLLVLR
                     ACDTLGLGRRAGEVVQGFDQVAAVLAGKDQGVLVEASDAAVHGARKLRARQGDGPLVA
                     CLTRAEIGRALGRDAVVHAWMRQGTLATRLLEEATRLEGFRHRQQAGPSAAGPADADD
                     QQGTSRAR"
     gene            19808..>22017
                     /gene="infB"
                     /locus_tag="P5558_06840"
     CDS             19808..>22017
                     /gene="infB"
                     /locus_tag="P5558_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRY24060.1"
                     /translation="MSDAKGQDRNTRIELGKGSAGGRLELKKAGEQGTVRQSFSHGRS
                     KAVAVEVKRRRGATAPAATPRPAATAPAEGQSTAAPAPRPVAAPTRPPAEALRQQRAV
                     TEARGSRSGRGPMVLPTLTEDEKRSRLRALAESKKIEEEARARAAENARRAADEAARR
                     KADEDAAAQRKAEEESRRKVEEEARKRAEEQAARLVADEEKRRAAAEAQATNQAAAPA
                     VLDADGELRAPEESKRGAKAARQLPVGEDEEGRGKGGKGDRRNTLRRTERDTRRNDKR
                     LILTTDEEAEEIERTRSLAALKRQRERERRQQSSGGTEPAVREVVVPEMITVQELASR
                     MAVRGAEVVKVLMRSGIMVTINQTIDADTAELVLSEFGQAARRVSESDVEIGLEGAED
                     DATKLQPRAPVVTVMGHVDHGKTSLLDALRQTDVVAGEAGGITQHIGAYQVTVGNGAR
                     VTFIDTPGHAAFTAMRARGASITDIVVLVVAADDGVMPQTIEAIRHAKAAKVPIVVAV
                     NKIDKPEANPDRVKQELLSHDVVVEDFGGDVLCVPVSALQKQGLDKLVEQLQLQAELL
                     ELKANPDREARGAVVEARLDRGRGVVATVLVQNGTLNQGDIVVAGASWGRVRSMSDDR
                     GETIKSAGPSVPVEIQGLDDVPEAGDQFVVVENEKRARDIADYRSRKQREQQQLKASP
                     ARSSLEEMFSQLKAGEIQSLPVVVKSDVHGSLEAIQAGLEKIGNEEANVRVLYGG"
BASE COUNT         3359 a         7853 c         7589 g         3216 t
ORIGIN      
        1 cgagcgtgcc ctgggcgacg atgccctgcc cctcgcggaa gaggtccggc aggatgccct
       61 gatagtggac ctcgatctcg ttcgccgtgt cggtcagccg aaaggcgacg ctgccgtcgc
      121 tctcgcgcct gatgctgccg tccaccacga ggccgccgac gcggaaattg cggtccacgg
      181 ccacctcacc accgacgatc tgggtcggcg tgacgaagaa cgcgatcttg tccccgagcg
      241 ctgtcagggt gagcgccgtg gcgccgccga gcaaaaggag ggcaaggccg atcagcacga
      301 ggcgctggcg tttgcgaaac cgcatctgcc ctcacgagcc ggacgaaggg ccgggctgcc
      361 cgtcggcgcg gacagcgacc agccgtcgca ccggacgccc ggcatcgccc gaccggaccc
      421 ggcggcgcag ctccatgagc tcggtgccgc gctgccgctg ctgccgccag ctctgccagg
      481 ccagcccgag cgtcaggacg agggtgaagc cgaacgccga ccagacatag ccgccgtagc
      541 cccccatttc gatgaactcc aagcgcgacc tcccaagaag ctcgtccgcc agccggatgc
      601 cgttcacgca ttcgccgcaa cacactatag atggaatctc gtgtaaaggc gtccaggcgg
      661 cggcgcgaga cgatcggacg cagcgcttgc cgcccctccc cccgtcctac ggttgaaggt
      721 gccgccaccc ctgtcatgtc gctgctcgcc cccgatctcc agccgcgccc cgagctgacc
      781 tgccgtcagc agatcggggc ccccctgccc gaggacgagc aggcggaatc cgcgacggcc
      841 gtgctggcgc ggatgcgcga gctgctgggt gagagcgagc ggcggctgga ggccgggttc
      901 gccgaggagg gcgatggcga gcgcgtcgtc cgcgagcggg cacggctgct cgacggtctc
      961 ctcgccggtg ccctcgattt cgccgagagc cagctctttc gggtcgccaa cccgacccgc
     1021 ggcgaggaga tggcgctgct cgccgtcggc ggctacggcc gtggcgagct ggcgccgcac
     1081 tccgacgtcg acgtgctctt tctctatccc tacaagccga cgccgcatct cgagcagatg
     1141 accgagttcc tgctctaccg gctctgggac ctggggctca aggtcggtca ggcgacgcgc
     1201 tctttgaacg aatgcatcaa gctcgccaag agcgatctca ccatccgcac caccctgctc
     1261 gagacccggc tgctctgggg ctcgcagcgg ctcacccgcg agctggaggg gcggtttcgc
     1321 gccgaggtct gcaagggcca cgagcccgag ttcatcgagg ccaagctcgc cgagcgcgat
     1381 gcccgccacc ggcgcaccgg cgacagccgc tacctgctcg agccgaacat caaggacggc
     1441 aagggcggcc tgcgcgacct gcacaccctg atctggctcg cccgcttcat ctacgacatc
     1501 gccgactggg acgacctcgt ccggcacggt gtcctgagcc gcgccgagct caacggcttc
     1561 ctccgctcgc ggcggttcct ctggaccgtc cgctgccacc tccactacct caccggccgc
     1621 gccgaggagc ggctgacctt cgacctgcag ccggagatcg cgcgccgcat ggggtatcgt
     1681 gaccggccgc gcacgctcgg cgtcgagcgc ttcatgaaga gctactacct cgtcgccaag
     1741 gaggtgggct cgctgacgcg gctggtctgt gccgcgctcg aggagcagca caagcggcgc
     1801 ccgcgcttcg ccctgccgcg cttcggcccg agccagcggc ggatcggcgg cttcgtcgtc
     1861 cagggcagcc gcctggcgct cgccgatccc aagctcttcc agacccggcc catcgccatg
     1921 ctcgagctct tccacacggc gcaggcccgc gagctcgaca tccatcccat ggcgctcacc
     1981 gccctggcgc agaacctgcg ccgcgtcgac cgccagctgt gccagagccc cgaggcgaac
     2041 cgcctcttca tcgagatgct gaccagccgc aaggacccgg cgcagacgct cacacggctc
     2101 aacgaggcgg gtgtcctcgg ccggctggtg ccggatttcg gccgggtcgt cgcccagatg
     2161 cagcacaatc tctaccacgt cttcacggtg gacgagcaca ccatccgggt gatcgacaac
     2221 ctgcacagga tcgaggaagg cacgctcgag gccgatcacc cgctgtcgac gcgcatcctg
     2281 ccgcagctga cgtcgcgcac cgagctctac gtcgccgcct tcctgcacga tgtcggcaag
     2341 ggccgcggcg gcgaccacag cgaggttggc gcgcgcatcg cagagaggct cggcccccgt
     2401 ctcggcctcg gccaggcggg cacggagacc gtcgcgtggc tcgtccgcca ccacctcgtg
     2461 ctcagcaatt tcgccttcaa gcgcgacagc gaggacccga agaccctgca ggatctggtc
     2521 gccatcgtgc agtccccgga gcgcctgcgc ctgctcctgg tcctgacggt ggccgacatc
     2581 cgcgctgtcg gccccggtgt ctggaacggc tggaagggcc agctcctgcg cgagctctac
     2641 cacgaggccg aggcggcgat ggcctcgggc gacctgcacg gccggcgccg ggagcggatc
     2701 gagcgggccc gcgccagcct ggccgaggcg ctcctcgccg gcggcgacgg cgaccggtgg
     2761 acgtcggccg aggtcgagac ctacaccgcc ctgcacgatc cgcgctactg gctcggcgtg
     2821 gcagaggacg cgcatctgcg gcatgcgagg ctggtgcgcc gggcgcaaac acagaagctg
     2881 ccgctggccg tcgatttcca tgtggacgag tttagggccc gcaccgagat caccgtctac
     2941 gcggccgacc atcccggcct cttcatgaag gtggcgggtg cgctggcgct gagcggtgtc
     3001 agcatcgtcg acgcgcacat cttcacgacg gcggacggca tggcgctgga cacgctcggc
     3061 gtccaggacg cggagcgcca ggcggcggtc gacgacgagg cgcgcttcgg ccgcatcgag
     3121 cgcaacatcc gcaaggtcct gcagggcgag gtctggctcg agaaggagct cgccggccgc
     3181 cgctccctgc caccccggac cgaggtcttc acggtggagc cccgggtgct cgtcaacaac
     3241 caggcgagca gcacgcacag cgtcatcgag gtcaatggcc gcgaccgccc gggcctcctc
     3301 ttcgccctgg ccaaggtcct gaaggagcgc ggcctcgtca tccacagcgc ccatatcagc
     3361 acctatggcg agcgggtcgt cgacgtcttc tacgtcaagg acgtgttcgg cctgaaggtg
     3421 gcgagcccgg ccaagctgaa gcggctccgg aacgagctcg aggcggcgct ggcggagccg
     3481 gccgacaggg cttccggcaa atcgggatga accacgcttg aacatcgtca aggcgacgct
     3541 gaccgtgggc tcgttcacgg cgatctcgcg cgtgctgggc ttcgtgcgcg atctcctgat
     3601 cgccgccgtg ctcggcgccg gagccgcggc ggacgccttc ttcgtcagct tcaagctcgc
     3661 caacctgctc cgccggctgt tcgccgaggg cgccttcaac gccgccttcg tcccgctctt
     3721 cgcccgggcg ctggagggcg agggcgagga agaggcccgg cgcttcgccg aggaggcgct
     3781 ggcggtgatg accgccgtcc tgctcgtcgt cgtcctggtc gccgagatcg ccatgccctg
     3841 gctggtgcgg ggcctggcac ccggcttcga gcccgctggc gagcgctacg gcctcgccgt
     3901 cgagctcagc cgcatcacct tcccctatct catgctcatc tcgctcgccg cgctcttcag
     3961 cggtgttctc aactcgctcg gccgcttcgc cgccgccgcc atcgccccca tcatcctcaa
     4021 cgtggtgctg atcaccacac tgctgctggc gagcctgcac ccggcgaccc cggcccatgc
     4081 cctggcctgg ggggtttccg tcgccggcgt gctgcagctc gcctgggtgg ccgttgcggc
     4141 gcgccgcaac ggcatgggtc ttaagcttcg ccggccacgg atgacccctc gcctcaagcg
     4201 gctcttccag ctcatgctgc cgggggcgat cggctcgggc gtctaccaga tcaacctgat
     4261 cgtcgacaca tggttcgcct cgctcctgcc ggtgggggcc gtctcctacc tcttctatgc
     4321 cgaccgcctg aaccagctgc cgctcggcat catcggcgtc gccatcggca cggcgctgct
     4381 gccgatgctc agccgccagc tccgcgtctc ggacttcgcc aatgcatcgc gcacgcagaa
     4441 ccgggcgatc gagatcggcc tcctcttcac cctgccggcg gcggccgggc tgatcgtcct
     4501 ggccgagccg atcatcgccg tgctcttcga gcggggtgcg ttcggcccgg ccgagacggc
     4561 ggccacggcc ggggcgctcg tcgccttctc gatcggcctg cccgcctatg tgctgatcaa
     4621 ggtcctggcg cccggcttct tcgcccgcga ggacacggcg acaccggtca aggtggcggc
     4681 cctttgcctc gtcgccaacg tgctcctcat cctcctcctg atcggcccgc tccgccatgt
     4741 cggcatcgcc ctggcgacgg ccctctcgaa ctgcctcaac gtcgcctgcc tcgcctggat
     4801 cctgcagcgc aggggccatc tgcacatcga cgagcggctg cgccggatcg tgccgcggat
     4861 cgtcgtcgcc acggtcgcga tggcgctgat cctcgtcgcc gcccgcctcg ggctcgactt
     4921 cctgcccgcg gccctgcgcc tgatggcggc catcgcgtcc ggcggcctct gcttcatcct
     4981 cgcctgccat tggacaggcg ccgcggattt cgccgagctc aggcgcatgg tctcgcgccg
     5041 gcggccggca gccgagcccg gcgcttgaca ctgcctcact gccgtggctc caagcggctc
     5101 catgctcgct cgcaagaggt tgccgtttcg atgcccagca cgtccaggaa ccgcattttc
     5161 tccggcatcc agccgacgca cggcattcag ctcggcaact atctcggcgc catccgcaac
     5221 tgggtgaagc agcaggacca gttcgagtgc atctactgca tcgtcgacca gcacgcgctg
     5281 acgacggtgc agaacgccga cgagatgcgc cgcaacacgc gcgagatggg ggcggcgctg
     5341 atcgcctccg gcatcgaccc ggcccgttcg gtgctcttcg tgcagagccg ggtcgaggcg
     5401 cacagccggc tcgcctgggt gctgaattgc gtgacaccct tgggctgggt caaccggatg
     5461 acgcagttca aggagaaggc cggcaagcag cgcgagaacg cgccggtcgg gcttttcgtc
     5521 tacccggtgc tgcaggcggc cgacatcctg gcctaccggg cgacgcacgt gccggtcggc
     5581 gaggaccaga agcagcacct cgagctcagc cgcgacatcg cggcctcgtt caaccgccgc
     5641 ttcgccgagg atttcttccc gctgccggag ccgatgatcc aggccgaggc ggccagggtc
     5701 atgtcgctca gggacgggac gaagaagatg agcaagtcgg atccctcgga catgagccgg
     5761 atcaatctca ccgacgatgc cgacaccatc gcgagcaaga tcaggaaggc caagtccgac
     5821 agcatcctcg gcatcagcta cgatccggag aaccggcccg aggcgagcaa cctgctcacc
     5881 atcttcgcgg ctctctccga tcgcgagcgc gccgacgtgg cggccgagta cgaatcggcg
     5941 agcttcgcca gcttcaagga gcatctggcc gagctctgcg tcgagaagct ggcgccgatc
     6001 aatgccgaga tgcgtggcct gctcgccaat ccggaccata tcgacgccgt gctcggcgac
     6061 ggctcggccc gggccgacgc cctggcccgc ccgattgtcg acgcggccta tgaagcggtg
     6121 ggctttcgct gaccggcccg cttgcgatgc ggcgccggtg acgccaagtc ttggcatggc
     6181 gagaccggcc gccgccgtgc cgggaccgcc agtcgacgac gccgcgcacg cgcccccagg
     6241 gccccgagga gacgacgcat gttcatccag accgaagtga cccccaaccc ggcgaccctc
     6301 aagttcctgc ccggccgtcc cgtcatgacc gaaggcgtgg tcgatttcac cgaggcggcc
     6361 aaggccgagg cggtctcgcc tttggcggcc aggctcttcg ccgtcgacgg cgtggccggc
     6421 gtctatctcg gcgccgactt catctcggtc accaagaatt ccgccgacga ctggtacatg
     6481 atgaagccgg cggtgctcgg cgccatcatg gagcacttca tgtcggatga tccggtgctg
     6541 gccgccggcg ccgacatcga cgacgacgtc aaggtcgacg aggccgacgc cacggtgaag
     6601 cagatcaagg agctgatcga cacgcgcgtc cggcccgccg tggcccagga cggcggcgac
     6661 atcgtcttcc agggcttcga gcggggcatc gtctacctgc acatgcgcgg cgcctgctcg
     6721 ggctgcccga gctcggtcgt gaccctgaag aacgggatcg agaacctcct caagtactac
     6781 gtgcccgagg tggtcgaggt gcgctccgtc tcctgacgac gaaagcgggt cgttgcggct
     6841 cctggccttc gataccgccg gcgacggctg ctcggccggc tgcttcgcgg gcgccgagcc
     6901 cgtggcgttc gcgcggcatg ccggcgccag cggccaggcc gagcggctcg tgccgatgct
     6961 ggcggcggtg ctggccgatg ccggctgggg catggaagac ctcgaggcgc tggcggtcac
     7021 gatcgggccc ggcagcttca ccggcatcag gacgggcctc gcagccgccc gcggcctggc
     7081 gctggtccgc cgcctgccgg cctggccggt cacaacgctg gcggcgttgg cggcagtggc
     7141 cgacggtgcc gatggcctgc cgctcgccgt ggtgctggac gccaggcgcg gccaggtctt
     7201 cctgcagcgt ttcgacagca gcggcgctgc cctcgacgag cccctgatgc tggcgcccga
     7261 gctggcggcg gcgcggctgc gcgagcccca ccatctggtc ggcagtggtg ccgtggccct
     7321 gctgcggcat ttggacgcga ccgccatgac cctgggcgac gccgtgctcg acgcggtcgc
     7381 ggtggcccgc gccgcggcgg cggcccgcgg gagaggcgag gcaccggtcc acgggacggc
     7441 gctggcgccg ctctatctgc gcgagcccga tgcccggccg tccgccggcc ggtcgctgct
     7501 gcaggcctga gccatgctgt cgttccatca ggagccgcgg ctgaccatca agccggtggg
     7561 tgccttcgat ctcggccggc tggcgcggct ccaccgccat tgcttcgagg aaggctggag
     7621 ccggtccgac ctcgcccatc tcctggccct gcccggcggc ttcgggctga tcgcccggcg
     7681 cggcgggacc tttgtcgggc tcgatgcgct caggggcgtc ggcttcgccc tcctgcgcgt
     7741 ggtccgcgac gagtgcgagc tgctgtcgct gggcgtcctg ccggcgcatc ggcggcgcgg
     7801 tgtcgcgggc gagctgctgc ggaccgccat gcagagctgc ctcgatggcg gcgcgcagtc
     7861 gatgtttctc gaggtggcga tcgacaatct ctcggcgcag gccctctacg accggttcgg
     7921 cttcgtccgg gtcggcacgc gacctgacta ttacgcccgg cccgacggca cgcggatgca
     7981 tgcctacacc atgcgctgcg atctcgtcgc agcactcggc agccgcgcgc cgcaaccacg
     8041 ctgagccggg cgcatttcct cattcggcca cgcgaatcac gagctcccag aggtcgtcgg
     8101 caatcgcctc ctcgacgatg atgctgtggc cgtgatcgag caccgcgcgc ggcacgttgg
     8161 ccagcggctc gccgccgcgc agccgcaccc gcaagagctc gcccggccgc attgcttcga
     8221 gcctgatctt ggtgcggaca aacgtcatcg ggcaggtctc gcccgaaata tccaactctg
     8281 cgtctgcctt cattccgcta cttgttccct tactgcgcgg cttcgacgga aacttccact
     8341 ttttcgcgaa tccctcttgc atccccggcg gcagaaaata tattgcggac accgaaagaa
     8401 tctatgcttg ggcgaaaggg ttaacgaggt atgtcggacc aattgagtca aaatgagctg
     8461 ctgtcgctga ctacggagat tgtggcgtcg cacgtctcga acaacaccgt cgccgtcagc
     8521 gacctgccgc agctcatccg gctcgtctac accggcctgg ccgagcaggg ccagacgaaa
     8581 gccgaggagg aaccggaaaa gctcgttccg gcagtgccga tcaagaagtc gatacagccg
     8641 gatcatctga tctgcctgga ggatggcaag aagctgaaga tgctcaagcg gcatctcaag
     8701 acgcgctacg acatgacgcc cgacgagtat cgtcggcgct ggggcctgac cgacgactat
     8761 ccgatggtcg caccggaata tgccgagcaa cgcagcagcc tcgccaagaa gatcgggctc
     8821 ggcaccaagc cgcgggcgcg gggacggaag gcggcctcct gagcccgggt ggccgtctgc
     8881 tctcggacgg aaggttgcgc ggatcgttca gggtccggtg cttttcgcgg gatcgacgtg
     8941 ggaaatggcg gacgggcggc ggggagcgcc gcccgttttg ccgttccggc gccgccatgg
     9001 taatgaattg tcaacgaact gtcgcgacag tatgggaagg aaggcgggtc ctgctggcag
     9061 ggcccgctgt cgaacaaacg ccgacgccga ggtgcattgc tgagaagtag ccaggatacc
     9121 gtgagactgc cggcgacgag ttccgccgga cgatcggccg gttcgagcag cggcccgacg
     9181 accagcgggc cggcggtcgg catgtggtgc cagcgacgcc catgacattc gggcaaaccg
     9241 cacccgacac cggcaaggcc gccccgaaga agctgttcat caagacctat ggctgtcaga
     9301 tgaacgtcta cgacagcgac cgcttggctg cattgctggc gcgacacggc tacgcgaccg
     9361 tcccggaggc cgccgacgcc gatctcgtcg tgctgaatac ctgccacatt cgcgagaagg
     9421 cggccgagaa ggtgtactcg gagctgggtc gtctgcgcgt gatgcgcgac cagcgccggt
     9481 ccgagaccgg ccgtcccatg gccctcgtcg tcgccggctg cgtcgcccag gccgaaggcg
     9541 aggcgatgct cgggcgggcg ccgttcgtcg atatcgtcgt cggcccgcag gcctatcacc
     9601 gcctgccgga gctcctggct cgcagcaccc gcgaagccgg cggccagatc gacacggatt
     9661 tcccgctcga gagcaagttc gaccttttgc cgcaaggtcc tgaggaatcc ccggtttcgg
     9721 ccttcctcac cgttcaggag ggctgcgaca agttctgcac gttctgcgtc gtgccctaca
     9781 cgcggggggc ggagagcagc cggcccgccg ccgccatcgt cgccgaggcg cggcgactgg
     9841 tcgagggcgg ggcgcgcgag ctgaccctgc tcggccagaa cgtcaatgcc tatcacggcg
     9901 acggtcccga cgggcgaagc tggagcctcg cccggctgct ccacgagctc gccgggatcg
     9961 agcgcctgga gcggctccgc tacacgacct cgcatccccg cgacatgacg gcggacctga
    10021 tcgaggcgca cggcgcgctg ccgcagctca tgccgttcct ccatctgccg gtccagtcgg
    10081 gcgccgatcg ggtgctgcag gcgatgaacc gcggctatgg ccgcgacgac tatctccgga
    10141 tcgtcgacgc cctgcgcgat gtccggccgg acatcgccct ctcctccgat ttcatcaccg
    10201 gcttcccggg cgagacagag gaggatttcg aggcgaccct ggcgctcgtc gagcgggtcg
    10261 gcttcgccca ggccttctcg ttcaagtaca gcccgcggcc gggcacgccc ggcgccgcga
    10321 tgacgaagca ggtgaaggag gccgtgcgcg gcgagcgcct gaaccggctg caggcgctgc
    10381 tcgacgccca ggccacggcc ttcaacgagg gaaccctcgg ccggacgctg ccggtgctgc
    10441 tcgagcggcc cggccgccat cccggccagc tcgtcggccg cagcccgtat ctgcaggccg
    10501 tgcatgtcgc cgcaccggac gggctgctcg gccgggtggc ccgggtcacc attaccggtt
    10561 gccatcggca cagccttgcc ggccgcatgg aaccacacgg gccgatcaca gcttcaaacg
    10621 ggatacccgc gcagccagag gaggtcgtcg cctgaccacc atagccccgc tcgccgactt
    10681 ccagaactcg agccatcggg tcagcttcga cgacaatgcc acctgcgcca tgctcttcgg
    10741 ccggcaccac atgaacatcg cccgcctcga gcagcggttg ccggtcagca tcgtcacgcg
    10801 cggcaacgag atcttcatcc agggcgagag cgacgccgac gtgcggacgg cggccactgt
    10861 cctagagcgg ctctatgagc agctgcaggg cggccattcg atcgatcgcg aggatgtcga
    10921 cgcggcgctg aggatggtcc agggcgacgt ggtggatgcc ggcttcgtcc gcgaggtgcg
    10981 gaccgatcgc cgctcgatca tgccgcgctc gcccaaccag gcggcctatc tgcggctgct
    11041 cgagcagcgc gatctcgtct tcgccctcgg ccccgccggc accggcaaga cctatctggc
    11101 ggtggccgcg gcggtgtcct tcatgcgcca gcgccgggtc gagcggatca ttctctcgcg
    11161 gccggcggtc gaggccggcg agcggctcgg gttcctgccg ggcgacctga aggagaaggt
    11221 cgacccctat ctccgcccgc tctacgacgc gctgcaggac atgctgccag acggcaagct
    11281 gcagacgcgc atcgagcagg gcgagatcga gatcgcgccg ctcgccttca tgcgcggccg
    11341 gacgctgagc cacgccttcg tcatcctcga cgaggcgcag aacacgacgc cggcgcagat
    11401 gaagatgttc ctgacccgtc tcggggaagg ctcgcgcatg gtcgtgaccg gcgacccgtc
    11461 gcagatcgat ctgccggccg gcgccccctc cggcctcgcc gacgcggccg cccgcctgca
    11521 gggtatccag gagatcggca tggtcgtctt cgacaagtcc gacgtcgtcc gccatccgct
    11581 ggtcacccgg atcgtcgagg cctatgagga cgagcagccg gctgccgccg gatgaccggc
    11641 cgccaaccga ccggtcagcg atcgacccat catcaatcga acccacgaaa gagcaggagc
    11701 cgacaggcgc ctgggaaggc gccgaagaac cgaaatggac agcgacagta gcccatctat
    11761 cgaggtggcg gtcggcaatg gcgcatggcg ctcggccgtc accgatcccg aggtcatctg
    11821 ccaccaggcg gccaccgccg ccttgcgagc cgcggccgag acctacttct caaccggcgt
    11881 gcccgaccgc ctcgaggtca gcatcaggct gaccgacgac gccgaggtgc aggcgctcaa
    11941 ccggacttgg cgaggtcagg acaagccgac caacgtcctg tccttcgagg gcgatgaccc
    12001 cggcctcgtc gacccccgca ccccttcgga cgcgccctgg ctgctcggcg acgtggtgct
    12061 cgcctgcgag accgtgacgc gcgaggccga ggcggacggc cggccggtcg cggcgcatct
    12121 cgcccatctc gtggtgcatg gcgtcctcca tctgctcggc cacgatcacg aggacgaggt
    12181 cgacgccctg acgatggagg ggctggagag ccgaatcgtc gaagggctcg gctatgccga
    12241 tccgcatggc gagcccgggc tgcagccggc gcccctcgcg gaggctcggc catgaggcag
    12301 ctcgcccgac gccaggacac cagcccgtcc atgctcgagg ggctgctgcg ccggctgcgc
    12361 gagctggcca gcggcgagag cagcacgggc ggcctgcgcg agaccctcga ggacctgctg
    12421 gaggaggagg attcgggccc cggtgcggag cagttcacgc ccgaggagcg ggagctggtg
    12481 ctcaacgcct tgagcttcgg cgagcttcgg gtcgacgacg tcatggtgcc gcgcgccgat
    12541 atccgcgcca tcgagatcaa caccggtctc gacgacgtcg tggcggcgat gcgcgaggcc
    12601 ggccactcgc ggctgctggt ctttcgcgac cgcctcgacg acgtggtcgg gctcgtccac
    12661 atcaaggatc tcctgccctt ctggggcgac ggcggcgatt tcgcgctgag cgcggtcatg
    12721 cgccaggtgc cggtcgtccc gccatcgatg cgcgtgctcg acctgcttct ggagatgcgc
    12781 aagagccacg cccacatggt ggcggtggtg gacgagttcg gcggcaccga cgggctggtg
    12841 accgtcgagg atctggtggt cgagatcctc ggcgagatcc atgacgagca cgcgccggac
    12901 gagccgccgc agctggtcca tcgggccgat ggcagcatcg aggccgatgg ccgggtggat
    12961 ctcgaggacc tcgaggagca gctcggccag acattgctcg atggcgacga gcgcgacgag
    13021 gtcgacaccc tgggcggcct gatcttcacc ctggtcgacc gggtgcccga tatcggcgaa
    13081 tcggtgcacc atccggtcgg gctcactttc cacatcctcg acgccgatcc gcggcgcatc
    13141 aagcgcgtgc gcatcgagat cgtggatccg gaggaacgcc agccgaggcg cctcgccggc
    13201 gagctgctcg ggccgggcga ttgagctcgg gcacggccgt ggcgggtcgc gtcccggccg
    13261 cggcggcgga gcggccggcc cggctctggc tgacgccgat tgccggcgcc gcactggcgc
    13321 tcgccctgcc gcctctgggc ttcctgccgg cggccctggc gcttggcctg ctcttcgtcc
    13381 agctacaggg cgcacgcggc tactggccgg ccttcgcgcg ggcctggctc ttcggcttcg
    13441 gcttccatct ggccgggctc tactgggtcg gcattgcctt cctcgccgag gccgaccggt
    13501 tcggcgccct ggcggtgccg ggcgtcgtcg ccctggccgc catcctcgcc gtgatcggcg
    13561 ccatgccact cgccctcctc ggccccctgc ggctgcggca gccgatcgcc gccgccgcgg
    13621 tgtttgccgg gctctggtgc ctgggcgagc tcgcgcgtgg ccagcatggg gtgcagttcc
    13681 cctggaaccc tctggcgctg gcgctggcgg cgaccgacac ctcgctgcag gtcgtggcgc
    13741 tcgtcggcac caccgccggc ggcttcctcg tcgcctggct cgccgcactc gtcggcgtcg
    13801 ccctgacggt gcgggcggcg cgccggacgg cgctcgccct ctgcctcgcc atggtcgcgg
    13861 gcctcgccgc cttcggcctc tggcgcctgg ccagccaccc gccgggcctt atggccgaac
    13921 cgccggtcac ggtgcggatc gtccaggcca acatcgccca gcaccacaaa tgggatccgg
    13981 cgctgcgccg aaactggttc gatcggcatc tcgacctcgc ccggcaaccc atccggccgg
    14041 atgcgccgga tcggccggac atcgtcgtct ggccggagag ctcggtgccc tattcgctgg
    14101 acgccgagcc ggaggtccgc ggcctcatcg gcgacgtcgt ccggcccggg ggagtcgtca
    14161 ttctcggctc caaccatttc gacggctccg tcgagccgcc gatcctgcac aacagcgtct
    14221 acgcgctcgc cggcgacggc gagctgctcg gccgctacga caaggtggac ctcgtgccgt
    14281 tcggcgagtt cctgcccatg cggccgctcc tcggcgcagt cgggctcgag gcgctcgccg
    14341 tcggcagcat ggacttccag gcaggcccgg ggcgcaccac catggccctg ccgggcctgc
    14401 cgccggcgag cccgctcgtc tgcttcgagg ccgcctttcc cggccgcggc accgacggct
    14461 ccggccgtgc ccggtggatc gtcaacgtca ccaacgacgc ctggttcggc atctcgagcg
    14521 ggccctacca gcacgctgcc atggcgcgga tgcgagcggt ggagaccggc ctgccgctgg
    14581 tgcgggccgc caatacgggc atctcgatgc tcaccgaccc catgggccgc gtcctggaca
    14641 gcctgccgct cggcaccacc ggcaccatcg acgccagcct ccccgccgcc ctgcccacct
    14701 ccccgccgat cacccggtgg ccctggctct cgtcgctgct gatgggcctc gccttgctgg
    14761 cggccggcgc cgccgagctt gccggccgca ggtcatcccc tcgagacagg gaccattgat
    14821 cgctgcagcg ccccgacaac gggtgccggc aatcgcgtcg cccggccatt ttggcgcctc
    14881 ccgtggcagc acgtcgcctc tcggggcttg aaccacagcc acttcatcgg cagaacaccg
    14941 ccaacccagc cagacggaca tcaccccaca tggtcgccaa cgattacctc ttcaccagcg
    15001 aatcggtctc cgaaggtcat ccggacaagg tctgcgaccg gatctccgac gaggtggtgg
    15061 acgcctatct cgctgccgag ccggaagccc gcgtcgcctg cgagaccctg accacgacca
    15121 accgcatcgt gatcgccggc gaggtgcgcg ggcccgccgg gctgacccac gaccacgtcc
    15181 gctgcatcgc ccgcgaggcg gtgcgggcga tcggctacga gcaggacggg ttccactggc
    15241 gcgatgccgc cgtggatgtc tatctgcacg agcagtccag cgacatcgcc atgggcgtcg
    15301 atgccggcgc cgacaaggaa gagggtgccg gcgaccaggg catcatgttc ggcttcgcct
    15361 gcgacgagac gccggacctg atgccggcgc cgatcctcta cgcccaccag atcctgcgac
    15421 tgatggcgga ggcgcgccat gcgggccaga cgcccgagct cgcccccgac gccaagagcc
    15481 aggtgacgct gcgctatgcc ggcgggcggc cgatcgcctg cacctcgctc gtcgtctcga
    15541 cccagcacaa ggcctcggcc ggccgcgagc aggtgcgcga gatcgtccgc ggcttcgcca
    15601 agcaggtgct gcccgagggc tggatgccgg ccgaggacga gttctacgtc aatcccacgg
    15661 gccagttcgt catcggcggg ccggacggcg acgccggcct cacgggccgc aagatcatcg
    15721 tcgacaccta tggcggggcg gcgccgcatg gcggcggcgc cttctccggc aaggacccga
    15781 ccaaggtcga ccggtcggcc gcctacgcgg cccgctacct cgcgaagaac gtggtggcag
    15841 gcgggctcgc caggcgctgc ctgatccagc tctcctatgc catcggcatc gccaagccgc
    15901 tctcgatcta tgtggacacg cagggcacgg gcgaggtcga cgaggcgcgc ctcgccgacc
    15961 tgatgatgga tctcgacctc agcccgcgcg gcatccgtcg ccggctgcag ctcaaccggc
    16021 cgatctacgc gcgcacctcg gcctatggcc atttcggccg gcagcccacc gccgatggcg
    16081 gcttctcctg ggagcagacc gatctcgccg acgagctcaa gcgcgccttc aactgatccg
    16141 acggccgagc cgccaaggcg gcagcgggtt ctctatgggc ggcggagcgg cccgggcctg
    16201 cgtccgggcc agcgcgagcg gctggaccgg ctgctgccgc ggctcgccat cgacctgccg
    16261 gccgccggcg cgctcgatcc ggcgagcctg ttcacgccgc cgcgccaaag ggtctggctg
    16321 gagatcggct tcggcggcgg cgagcacctc gcggcacagg cggccgccaa cccgaccgtc
    16381 ggcctgatcg gcgtcgagcc tttcgtcgac ggcgtggcgc gcctcctggc ccaggtcgag
    16441 gagcggcagc tccacaatgt tcgcctgctc gtggacgacg cgcggctgct gctcgaccgt
    16501 ctgccggagg cgtcgatcga gcggctcttc gtcctcttcg ccgatccctg gccgaagaag
    16561 cggcactgga agcggcggat cgtgaatgcc gagacggtgg cgcgcttcgc cgaccttctg
    16621 gtcgacgatg gcgagctccg ggtcgccacg gacgatccgg gctaccgccg ctggatgctc
    16681 gcctgcctgc tcggcgagca gcggctcgac tggtgcgtgc gcggccccga ggactggcgc
    16741 gaccgcccgc cggactggcc gccgacgcgc tacgagaaga aggccctggc ggccggccgc
    16801 cggccggtct ttctgcggtt ccgccgcaag tcgcgcaccg cagagcttgc aacatcggcc
    16861 gaatgatccc ataacccgga tagatctcgg cttgggaaag cagagtgggt ttcggcccgc
    16921 tcttttttgt tttggcgcca gattcccgca ccgcaggcgt cgagccgagg tcttgcgaca
    16981 agttgacagg gtttttgcac ggaccgaatg acgacggcac acgaaggatc aaggatcgag
    17041 cccctcgccg cgctgctggc gccgacgctg cacgatctgg gcttcgatct ggtgcaggtg
    17101 cggctgatcg gcgggcagcg gcggacgttg caggtgatgg ccgagcccct ggaccggggc
    17161 gtcacgatga cggtcgagca ttgcgccgac atcagccagg ccgtgtcggc ggtgctcgac
    17221 gtggccgacc cgatcgccgg cgcctatgtg ctcgaggtgt cgtcgccggg cctggatcga
    17281 ccgctggtcc ggctcgacga cttcgcgcgc tttgccggtg acgaggtgcg gctcgaggtc
    17341 gagccgcctc tggacggccg caagcggtgg cgcggccggc tcgagggtgt cgccggcgag
    17401 gacgtgctct tgactgtcga tgggctgacc cggcgggtgc cgtacccgac catcaggaag
    17461 gccaagctgg tattgaccga cgcgctcgtg gcggccgccc tcaagggtgg cgccgggcag
    17521 gaggcgaggg agtaggcgat ggaactcagc accgcattgg gacgggccga gctgttgcgg
    17581 gtcgccgaga cggtggccca ggagaagggg atcgacaccg ccgaggtgat cgacgccctg
    17641 gagcaggccg tgcagaccgc cgcccgccgc aagtacgggc tcgagcacga catcatcgcc
    17701 gagatcgatc gcaagaccgg cgagatcagg ctgttccggc agctcgaggt ggccgaggtc
    17761 gtcgaggact acgccaagca gctcagcctc gacgaggcgc gcgaccgcaa cccggcggcc
    17821 cagcccggcg acctcattct cgatgccttg ccgccgatcg atctcggccg catcgcagca
    17881 cagagcgcca agcaggtcat cgtccagaag gtgcgcgacg ccgagcgcga gcgccagttc
    17941 gacgagttca aggaccggat cggcgagatc gtcatcggcg aggtcaagcg caccgagcac
    18001 ggcaacgtcc tggtcgatct cggccgggcc gaggcgatcg tgcgccgcga cgacctgatc
    18061 ccgcgcgaga gcttcaggaa caaggaccgg atcagggcct atctctacga cgtgcggcac
    18121 gagacccgcg gcccgcagat cttcctgtcc cggacgcacc cgcagttcat ggcgaagctc
    18181 ttcgagcagg aagtgcccga gatctacgac agcatcatcg agatcaagtc ggtcgcccgc
    18241 gatcccggga gccgggccaa gatcgcggtg atctcgaagg acagcagcat cgacccggtc
    18301 ggcgcctgcg tcggcatgcg cggcagccgc gtccaggcgg tcgtgcagga gctggcgggc
    18361 gagaaggtcg acatcattcc ctggtcgccc gaccccgcga ccttcgtcgt caatgcgctg
    18421 gccccggccg aggtgagcaa ggtcgtgctc gatccggaag ccaatcgcat cgaggtcatc
    18481 gttcccgacc acatgcagca catcgccatc ggccgccgcg gccagaacgt gcggctcgcc
    18541 tcgcagctca ccggctggga catcgacatc gtctccgagg gcgaggattc cgagcggcgc
    18601 aaccgcgagt tccgcgaggc caccgagctg ttcatcgaga gcctgaacgt cgaggaggtg
    18661 atcgcccagc tgctcgccac cgaaggcttc gccaatgtcg aggacgtggc ctacagcgag
    18721 acggacgagc tggccgccat cgagggcttc gacgaggaga tcgccgaggc gctcaaggag
    18781 cgggccgccg cgttcctcga ggagcgcgag caggaaatga acgccgaggc cgacgagctc
    18841 ggcatcggcg aggacctgcg cgccttcacc ctgatcgatc tcaagacggt cgtggccctc
    18901 ggtcgccagg gggtgaagag cctcgaggac ttcgccgatc tcgccaccga cgagctggtc
    18961 gagatgctcg ccgccgaggc gccgatcgac gaggtgacgg ccgccgatct catcctccag
    19021 gcccgcctgg tgctgggctg gatcgagccg gagccggagc ccgaagagct gctcgaggcg
    19081 gcggacgcga ccccggacga tggcacggcg gagaccacac actgaccgtg atgctcgcca
    19141 ccgccccgac cgacagcccg acgggcggcg cccttccggc cccgccccgg cggcgcgaca
    19201 tcgtcagccg cgaaagcggc gaccggtcgc gcatgctgcg cttcgtcgtc gatcccgagc
    19261 ggcgcctcgt gcccgatctg gcggcccgtc tgccgggccg cggcatctgg gtcatcgccg
    19321 accgggaaag cctcgcccag gcggtgaaga agcggctgtt cgacaggcag gccggcggcc
    19381 cggtggtcgt gccggaggat ctgggcgggc tggtcgagcg tctcctggtg ctgcgggcct
    19441 gcgacacgct cgggctcggc cggcgagccg gcgaggtcgt gcagggcttc gaccaggtgg
    19501 cggcggtcct tgccggcaag gaccagggtg tgctggtcga ggccagcgac gcagccgtgc
    19561 acggtgcccg caagctgcgc gcgcggcagg gcgacgggcc gctcgtcgcc tgcctcacgc
    19621 gggccgagat cggtcgagcg ctcggtcgcg atgcggtcgt gcatgcctgg atgagacaag
    19681 gtacactagc gacgcgtctg ctcgaggagg cgacgaggct cgagggcttc cgtcaccggc
    19741 agcaggccgg gcccagcgct gcgggccccg ccgacgcgga cgatcagcaa gggacatcga
    19801 gagcaagatg agcgacgcca aagggcagga tcgaaacacg cggatcgagc tcggcaaggg
    19861 ctcggccggc ggtcgcctcg agctcaagaa agccggtgag cagggaacgg ttcgccagag
    19921 cttcagccat ggccgcagca aggcggtcgc cgtcgaggtg aagcggcggc gcggtgccac
    19981 ggcacccgcc gccacgcctc gtccagccgc gacagcgccg gcggaaggtc agtcgaccgc
    20041 agcgccggcc cccaggccgg tcgccgcccc gacgcggccg ccggccgaag cgctccgcca
    20101 gcagcgcgcg gtgaccgagg cgcgcggctc acgcagcggg cgcggcccga tggtgctgcc
    20161 gacgctgacc gaggacgaga agcgctcgcg cctgcgggcc ctcgccgaat cgaagaagat
    20221 cgaggaggaa gcccgcgccc gcgccgccga gaacgcgcgc cgcgccgccg acgaggcggc
    20281 ccggcgcaag gcggacgagg acgccgcggc ccagcgcaag gccgaggaag aatccaggcg
    20341 gaaggtcgag gaggaggcgc gcaagcgcgc cgaggagcag gccgcacgcc tcgtggccga
    20401 cgaggagaag cggcgggcgg ccgcggaagc gcaggcgacc aaccaggctg cggcaccggc
    20461 ggtgctggac gcggatggcg agctgcgggc acccgaggaa agcaagcggg gggccaaggc
    20521 tgcccgtcag ctgccggtcg gcgaggacga ggaaggtcgc ggcaagggcg gcaagggcga
    20581 tcgccgcaac acgctccggc ggaccgagcg cgacacccgg cgcaacgaca agcggctcat
    20641 cctcaccacc gacgaggagg ccgaggagat cgaacggacc cgcagcctcg cggcgctcaa
    20701 gcgccagcgc gagcgcgagc ggcgtcagca atcgtccggc ggcaccgagc cggcagtccg
    20761 ggaggtcgtg gtgccggaaa tgattaccgt ccaggagctc gcctcgcgca tggcggtgcg
    20821 cggtgccgag gtggtcaagg tcctgatgcg cagtggcatc atggtgacca tcaaccagac
    20881 gatcgacgcc gacaccgccg agctggtgct ctccgagttc ggccaggcgg cacggcgggt
    20941 cagcgagagc gacgtcgaga tcggcctcga gggtgccgag gacgacgcca ccaagctgca
    21001 gccgcgggcc ccggtggtca cggtcatggg ccatgtcgac cacggcaaga cctcgctgct
    21061 cgacgcgctg cgccagaccg atgtggtggc cggcgaggcc ggcggcatca cccagcatat
    21121 cggcgcctat caggtgacgg tgggcaacgg tgccagggtg accttcatcg acacgccggg
    21181 tcacgccgcc ttcacggcaa tgcgcgcgcg cggcgcctcg atcaccgaca tcgtcgtcct
    21241 ggtcgtggcc gccgatgacg gcgtcatgcc gcagacgatc gaggccatcc gccacgccaa
    21301 ggcggcgaag gtgccgatcg tcgttgccgt caacaagatc gacaagcccg aggccaatcc
    21361 cgatcgcgtc aagcaggagc tcttgagcca cgacgtcgtg gtcgaggatt tcggcggcga
    21421 cgtgctgtgc gtgccggtga gcgccttgca gaagcagggt ctcgacaagc tggtcgagca
    21481 gctccagctg caggccgagc tgctcgagct caaggccaat cccgaccgcg aggccagggg
    21541 tgcggtcgtc gaggcgcggc tcgatcgggg tcgcggcgtg gtggccacgg tgctcgtcca
    21601 gaacggcacg cttaaccagg gcgacatcgt cgtcgccggt gcctcctggg gtcgtgtccg
    21661 ctcgatgagc gacgaccgcg gcgagacgat caagtcggcc gggccgtccg tgcccgtcga
    21721 gatccagggt ctcgacgacg tgcccgaggc cggcgaccag ttcgtcgtcg tcgagaacga
    21781 gaagcgagcc cgcgatatcg ccgactaccg cagccgcaag cagcgcgagc agcagcagct
    21841 caaggcatcg ccggcgcgca gctcgctcga ggagatgttc tcgcagctca aggctggcga
    21901 gatccagagc ctgcccgtcg tggtcaagtc ggacgtgcac ggctcgctcg aggcgatcca
    21961 ggccggcctc gagaagatcg gcaacgagga ggccaatgtc cgcgtgctct atggcgg
//
LOCUS       DAOTFI010000049        21961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2825_length_21961_cov_6.064822, whole genome shotgun sequence.
ACCESSION   DAOTFI010000049 DAOTFI010000000
VERSION     DAOTFI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21961
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2825_length_21961_cov_6.064822"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="P5558_06845"
     CDS             <1..831
                     /locus_tag="P5558_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HRY24061.1"
                     /translation="YRDATARANLLRALDLGVEVVGGIPHFERTMEDGRRSVVDLCEI
                     AAERGLLVDLHCDETDDPHSRHIETLAAETARLGLGGRVTGSHLTSMHSMDNYYVSKL
                     LPLIAEAGVAAIANPLINIVIQGRHDTYPKRRGLTRIAELQAAGVPVAFGHDCVMDPW
                     YSLGSGDMLEVAHMAVHAAQLTSREAMRAAFAAVTSVPAGILGLDGYGLEPGCHADLV
                     LLDAEDPIEAIRLKATRLVVVRRGRIVAETPAAVSRLHLPDRPPSVSARRSAPESSAH
                     "
     gene            886..1218
                     /locus_tag="P5558_06850"
     CDS             886..1218
                     /locus_tag="P5558_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24062.1"
                     /translation="MLSSRPLDEMSLAMVNRRALNRFAWILGCGLALALSLPSILFAA
                     TFGSFTGIGAGIVATVALLAREEPLAPHLTRWDIAAALYAASLFAGLFVDVEGVRHYL
                     LMQQHGFP"
     gene            1661..4990
                     /locus_tag="P5558_06855"
     CDS             1661..4990
                     /locus_tag="P5558_06855"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY24063.1"
                     /translation="MLAFNRTDLERLFSEAIWLKAEHLVGDGAVVDVSVERDGKSITG
                     QIKGGRRTPFLTRIKIANGRGGRVRLSSTCSCFYPECEHAAATLLGLLDQSTTLDPED
                     GSVVVEAELESWVAAVNAATRNGHADAADVVLYLLEPAQRSWRDGSLAQPVLINTARA
                     KRLRSNAYGRENPIAIANLLADPAPGYVGLQDQVIGRLLGGGTGQSRRLGSRADGEAL
                     IRVLETGRCHWRNAAGPPLRFVAEPRRGGFFWRFDSEGQQHLVCEVDDRHDDLVVVAL
                     GEPWYIDLATSEVGRVETGVDAALAGHLVNAPAIPAAAATIVRQRLIPSGERLPLPEP
                     LKKRERLETKPQPILLLHCPSLVVHRGTGWRREEQEVDCPLARVAFDYAGAEIGWQDG
                     RPEINHVQNNRLLVLPRDTIAEVQAIERLNTLGLQPLGPTGLGRFAPEPLRQDFTFEE
                     DEDDDVSVRWVEFNHIDVPKLRGEGWRVVYDETYPFQVIDKPVSWQIDLNDSGIDWFE
                     LDVGIELDGERIPLLPVLLDLFQRSPDDMTPAALAAYGEEPVYGTLPDGRLLPIPAAR
                     LMAMLEALYEQFVNNRVDPQGRLRLAPAEASRLTVIDRTMTEDEVVWTGGERILRLAR
                     QLGDNDRIPTVEPPRALQAKLRPYQQQGLSWLQFLASVGLSGVLADDMGLGKTLQTLA
                     HILAEKEGGRLTRPALIVAPTSLIPTWRSEARRFAPSLNMLTLHGNNRGDLFRRIERS
                     DIVLTSYALMLRDRDELVRRTYRMVVLDEAQAIKNPATKLAKVSCELQADHKIALSGT
                     PMENHLGELWSVFHFLMPGFLGDREAFRRIFRQRIEKDGDADRQALLVQRVRPFLLRR
                     TKEEVASELPPKSEFIREIELSDDQRDLYESVRLAMHARVREEIEQRGLARSNIAILE
                     ALLKLRQICCDPRLLKKRDGPAPASAKYEMLMSMLEGMVETGRRIIVFSQFVEMLELI
                     VEGLEEQGIGYTILTGRTRDREAPVKKFQNGEVPVFLISLKAGGTGLTLTRADTVIHY
                     DPWWNPAVEAQATDRAHRIGQDKTVFVYKLVASGTVEEKMVELQERKRSLVEGVLGGV
                     KGGGVGLSETDIEALFAPLPD"
     gene            complement(5000..5542)
                     /locus_tag="P5558_06860"
     CDS             complement(5000..5542)
                     /locus_tag="P5558_06860"
                     /inference="COORDINATES: protein motif:HMM:NF016180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HRY24064.1"
                     /translation="MADHEKAAGIELRPRPELGKLTLRATDEVAKLAADVLGRPWPAA
                     INRAAGEVDDRTLRLGPDEFLMLVARTTVAGLVDRLRPALADHHHALVDVSARLTALE
                     LSGPMVRQALAAACPLDLHDSSFAVDAATRTMLGKAEIVLDRLAADRWRLAVNRSLAP
                     YVEALLHEAGREFGVRTRSP"
     gene            complement(5535..8438)
                     /locus_tag="P5558_06865"
     CDS             complement(5535..8438)
                     /locus_tag="P5558_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY24065.1"
                     /translation="MAENFRLAEGGRIDRERPLRFRFNGMEMQGFAGDTLASALLANG
                     IHLVGRSFKYHRPRGIMTAGPEEPNALLQLDRGGGRSDPNMRATVVELIDGLDARSQN
                     HWPSLGFDIGAINDLLSPLLPAGFYYKTFKWPASFWKSVYEPRIRAAAGLGVAPTEPD
                     PDRYLHRHAHCDVLVVGGGPAGLDAALMAGRAGKRVILADEQSELGGGWLARPDVPLA
                     LAGRLTELATLPEVTLLTRTTVTGYYDHGYVVALERVTDHLADPDPDLPRQRLWRIRA
                     REVVLATGAIERPIVFTGNDRPGVMLASAARAYLNRFAVRPGTRAVVFTNNDTAYVAA
                     LDLAARGVGIACIVDARANPEGAHVAAARAAGIRVVTNAVVAGTEGRRRITQAAVARL
                     AGDGGLAGHLGEPMACDLLLVSGGWNPNVSLFSQSRGRLRFDERLQAFVPGEAVQATS
                     CVGACAGDFSGFDGDPAPLRPLWAVPADRPSVRAKAFVDFQNDVTAKDLGLALTEGFR
                     SIEHVKRYTTTGMGTDQGKTSNVNALALVAEKLAKPVADVGTTTFRPPYVPLAFGAIV
                     GHSRGALFDVVRRTPMHEIAAAKGAVFEDVGTWKRAWYFPKPGEDMHAAVARETAAVR
                     RTAGLLDASTLGKIDVQGKDARTFLDRVYTNAWSKLAPGMCRYGLMLGDDGFVMDDGV
                     TACLDDRHFHMTTTTGGAARVLAWLEDLLQTEWPGLEVYLTSVTEEWAAIAINGPKAR
                     DVLAPLVEGCDLAADAFPHMAWRPARIAGIAGRIFRISFTGELAFEVNVPADRGAEIL
                     EALLEAGRAHDVELYGTEAMHVLRAEKGFIIVGQETDGTVTPLDLGMDWIIAKKKGDF
                     IGKRGLTREDLQHPDRRQLVGLLTAEPERVLEEGAQLTEAANPAVPARMVGHVTSAYH
                     SPNLGRSIALALVEGGHGRHGQTLHVPMPSGQSIAALVTKPLFIDPEGSRLHG"
     gene            complement(8438..8722)
                     /locus_tag="P5558_06870"
     CDS             complement(8438..8722)
                     /locus_tag="P5558_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HRY24066.1"
                     /translation="MLLIDCPHCGPRAEIEFRCGGEAHIARPASPEETSDAAWRDYLY
                     LRRSPKGLHLERWNHIHGCQRWFNVARDTVSDEILAVYPMGSPPPDLGRT"
     gene            complement(8733..9983)
                     /locus_tag="P5558_06875"
     CDS             complement(8733..9983)
                     /locus_tag="P5558_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HRY24067.1"
                     /translation="MQRYSIVSLARNALTGHRNWPQAWRSPEPRPEYDIVIIGAGGHG
                     LGTAYYLAKEHGITNVAVIEKGWLGGGNTGRNTTIIRSNYLYDESAAIYDHSVRLWEG
                     LSAELNYNVMFSQRGVMMLAHNTHDVQVLKRHIHANRLNGVDNEWLTPEAAQAFCPPL
                     SIDRHARYPVVGAALQRRGGTARHDAVAWGYARGADARGVDIIQNCPVTGIRREGDRV
                     VGVETAKGFIAARRVGVVTAGSTPVVMGMAGVRMPLESYPLQALVSEPVKPVFPCVVM
                     SNSVHAYISQSDKGELVIGAGTDQYVSYSQRGGIQILEHTLEAILELFPMFSRMAMLR
                     QWGGIVDVTPDRSPIIGKTPVEGLFVNCGWGTGGFKATTGSAHVFAHTLATGEPHKIN
                     APFTLDRFRTGRLIDEAAAAAVAH"
     gene            10263..10685
                     /locus_tag="P5558_06880"
     CDS             10263..10685
                     /locus_tag="P5558_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY24068.1"
                     /translation="MEPTLYLFFKGNCLEAMTHYAETLGGKVGDVFRNGDAPDPESRM
                     PGGDDMVMNMSMQLGNATVMASDNSDGMYEKPRGFAVSIAPPSLAEFERIHAALAEDA
                     QSVAMAPGETFWAERFSMFTDRYGTPWMLNYEGDKAQS"
     gene            10698..11180
                     /locus_tag="P5558_06885"
     CDS             10698..11180
                     /locus_tag="P5558_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY24069.1"
                     /translation="MTELVTCLWFDHGEARKAAEFYAATFPDSQVGRVNAAPGDFPGG
                     QEGSELTVEFTVLGRKFVGLNGGPSFTPDEAISFMVVTDDQEETDRYWNAIVGNGGAE
                     SACGWCKDRWGFSWQITPRLLLELTTSADRAKARRAFDAMMRMRKIDLAALEAAVAKS
                     "
     gene            11218..11583
                     /locus_tag="P5558_06890"
     CDS             11218..11583
                     /locus_tag="P5558_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HRY24070.1"
                     /translation="MAYVDGFVIAVPTANRRRFIDHANKANTVFMELGATRVLECWGD
                     DVPKGETTDFRMAVKAKEDETVVFSWVEWPDKATRDAAMGRMRELMKTDDRCNPEKNP
                     MPFDGKRMIFGGFEPVVEL"
     gene            11699..12691
                     /locus_tag="P5558_06895"
     CDS             11699..12691
                     /locus_tag="P5558_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HRY24071.1"
                     /translation="MQVVTAAGAAVDTETVAFFLVPDFSMISFASAVEPLRIANRLSG
                     RSLYRWLLLSKDGGPVRGSNGISVNVDMAMDRIQASFGQSLPTICLASGLHGERYADK
                     DVFAWLRRMDRHGAVIGALCTGAHILAKAGLITDHRCTIHWENLPGFVEAFPDIDVAA
                     DLYEIDRNRFTCSGGTAPLDLMLSRIAAAHGEELATKVSEQCLLDRIRGPHDRQRMPI
                     RIRLGVHHPKLINAIELMEANIEEPLPQEQLAHYIGLSRRQLERLFRRHMGRTPAQYY
                     LELRLERARHLLYQTDLPIMSIACACGFVSASHFSTCYRQMFGRTPRAERNVAA"
     gene            complement(12961..14013)
                     /gene="tsaD"
                     /locus_tag="P5558_06900"
     CDS             complement(12961..14013)
                     /gene="tsaD"
                     /locus_tag="P5558_06900"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRY24072.1"
                     /translation="MAIVLGIETSCDETGVAIVDGERRVLAQSLFSQEDEHKLYGGVV
                     PEIAARAHLLRLDRLIERTMAEAGLDYAGLDAVAVTAGPGLIGGLMVGIVTAKAICLA
                     TGLPLVAVNHLEAHALTARLTHGLAFPYTLLLVSGGHCQLQLVEGVGRYTRLGSTIDD
                     AVGEAFDKVAKMLGLGHPGGPLVEAAAQGGDPHRFRLPRPLYAQPHCNFSFSGLKTAV
                     RQVILAADAVDDAFVADLCASFEAAVADCLADRTARALQRQAGTAAARQLVVAGGVAA
                     NRHIRARLQELAVAHGAGLVIPPPALCGDNGVMVAWAGIERLALGLTDGLDVAARPRW
                     PLDPEAEPLGGAGVKA"
     gene            14076..15029
                     /gene="hemC"
                     /locus_tag="P5558_06905"
     CDS             14076..15029
                     /gene="hemC"
                     /locus_tag="P5558_06905"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRY24073.1"
                     /translation="MDKGRKLKIGTRGSPLALAQAELTRQALADARPAALAVPLEATE
                     IRVIRTTGDHILDRPLAEVGGKGLFTKEIEEALLAGEIDLAVHSMKDMPTRLPEGLVI
                     GAMLPREDPRDALILGPELAGRCHGIAGLPQGARIGTASLRRMAQLKAARPDLVVETL
                     RGSVGTRLGRIAEGRFAGTLLALAGLQRLGMADRASTVLGIREMLPAVAQGAIGIECR
                     ADDAWLVERLAAIGCRATAACVDAERAALAALDGSCRTPIAGLAGIDGTELLLQVLIA
                     RPDGSTVHRETGKAHVMDGVALGEALGRKLRERGGPDFFRD"
     gene            15039..15743
                     /locus_tag="P5558_06910"
     CDS             15039..15743
                     /locus_tag="P5558_06910"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRY24074.1"
                     /translation="MHVLVTRPEPQATRTAELLAERGHRTHAAPLAVIEPLEAELPAA
                     VAVDAVLLTSANAVPALQAYLHLPVYTVGDATAAAARAAGAGQVVSADGDWVRLAALL
                     RGPAGPPAGSRLLHLSGTVIAGDLAGAVRAAGFACDRRTVYTIRPATTLPPELLRLLA
                     VGTLDAALFFSPLHARIWCRLVAKATMEAELAGIRAVCLSEAVAQPLRELGWRSVEVA
                     AAPTLPALIDCLEGPG"
     gene            16468..17622
                     /locus_tag="P5558_06915"
     CDS             16468..17622
                     /locus_tag="P5558_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24075.1"
                     /translation="MQGPPPLAPEAAQRVAALESSATTLDEQLAAATEQLSTANEQIT
                     NLNRLSAELESTVESAVAAAVERIPSRDGAAIATVGERLDEVSSKVDELVASAAAATD
                     PAPLLAAQAQRLEALQAALTSLDNRLASEVLRLEAARTETATGLQASIDETAAAVETA
                     RQAAKDELEAARTTLAEGIAAFETDLEAVRADIASLEATVADMQLARNRAAAAALLVR
                     DIDHSITAGEPFAEPLDRLQAMGTGNAELEATLASLAPAAASGVPTIDELRQGLVELE
                     ARQAAPPIGGSELLGGTVGNILGLVEVRDRDTDGMAATGLLGEADAALRRGDLAAAMS
                     HVEATRDMPDGPDPAAIDAWLEQARARATAVAALAALDAQIRDLLTATVN"
     gene            17652..19151
                     /locus_tag="P5558_06920"
     CDS             17652..19151
                     /locus_tag="P5558_06920"
                     /inference="COORDINATES: protein motif:HMM:NF018875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HRY24076.1"
                     /translation="MLRLIVFLIVAAALAWVAVWLANSPGEVVLRWEATDTVLPVGIA
                     VAIVAAISAAAIVVYEVWRWLAQLPRRVRSNRQQKRQLRGYQELSHGLIAAAAGDVTG
                     ARAHTRQAEKLLQANAATLLLSAQTAQLEGKEDVAQLKFNQMLKRPQTEFLGIRGLLA
                     DAVKRGDQKEALALARRAYERSPNTSWVLTTYFDLLTRSGKWADALKLVNDLAREKLV
                     SGPEATRRRALMKHMLAVEAVDEKRYAEALAQARKATGLAPTFAPAAVTAAQAANGQG
                     KLRLARRLLEDCWRLEPHPELASAYAALVPDETAAERLRRLGRLESLVPNHLTTQLLM
                     AELAMNARQWDTAKRHLDKALGLEPTAGAYRLYAEYERASGGGDAKARDWLAKAADAP
                     ADKVWVCEDTGEVLAEWQLFGPSGRFDSVHWDSPPKVAPMIRDHRPPVVLVHDQRAVG
                     EPAEPVTEVDDVKKEKDKAPGTSKTGATEQGLKAPPPIEATVKAAGSVG"
     gene            19218..19293
                     /locus_tag="P5558_06925"
     tRNA            19218..19293
                     /locus_tag="P5558_06925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19251..19253,aa:Thr,seq:cgt)
     gene            complement(19495..19743)
                     /locus_tag="P5558_06930"
     CDS             complement(19495..19743)
                     /locus_tag="P5558_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24077.1"
                     /translation="MTEEERNELITARGTGLAAMACIQQLCRVLQERGIMTTEQLHDA
                     ISEALAMVQTLEPKRPIEAEINRVAAVTIRNAFEDRRG"
     gene            complement(19875..20198)
                     /locus_tag="P5558_06935"
     CDS             complement(19875..20198)
                     /locus_tag="P5558_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24078.1"
                     /translation="MRQTDYRELALTGRARPGSNMVVVTRPRLEDGDPAREAAFKLAQ
                     DALDEWAALPDDIDPKALAAARDALREALGILGAVTGRAAAPSPYVGRRPVVAPSAGV
                     AALLR"
     gene            complement(20195..21280)
                     /locus_tag="P5558_06940"
     CDS             complement(20195..21280)
                     /locus_tag="P5558_06940"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRY24079.1"
                     /translation="MRWWPFRRQKIERRASGSGFTAEIMAARESYISGRRGLGELTAT
                     VQGAISLWEGALALADVTGTDMLPRSLMALLARSVALRGEAVFLIRDDRLVPCSDWDL
                     RTRDGVPTAYRISLSEAGGGSTQTALAAEVIHIRIGSDPAAPWLGTAPLRRASLTGEL
                     LHAVEAALAEVFAQSPIGSQIVPFPEAPETDLQALGRSFRGQRGKVVLRESVNVSAAG
                     GPAPAADWRPSSISPDLSRSMTAETLAAARDSILSAFGVLPALLNPAATGPLIREAQR
                     HLAGWTLQPIAELIAEEAGAKLGGPVKIDVMRPLQAWDAGGKARAFSGMVEAMASAKA
                     AGLEPAEVAKLLSLVGWADDIDRGPLQ"
     gene            complement(21277..21885)
                     /locus_tag="P5558_06945"
     CDS             complement(21277..21885)
                     /locus_tag="P5558_06945"
                     /inference="COORDINATES: protein motif:HMM:NF022876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3199 family protein"
                     /protein_id="HRY24080.1"
                     /translation="MLDARTAGRAEPAGGDAAVAAMTRATVLSITEGQPASWPAIPGF
                     TGTVADAAATWQRLEAWISHRWPARPVVMIVEGPGTFLPPLAPLSIATVEGWAGGGTW
                     ASASLAEMAAPLGYALDADTSFRFTGTVGDDTDPPAVVLEAARRLAEFFASIAAEPST
                     MAGYTSETLGDYSHTGRPSAWAARAMVLSGAADLLRPWRALR"
BASE COUNT         3327 a         7664 c         7751 g         3219 t
ORIGIN      
        1 tatcgggacg cgaccgcccg ggccaacctg ctccgggccc tcgatctcgg ggtcgaggtg
       61 gtgggcggca tcccgcattt cgagcgcacc atggaggacg gtcggcggtc ggtcgtcgat
      121 ctgtgcgaga tcgcggccga acgtggcctt ctggtcgacc tgcactgcga cgagaccgac
      181 gacccgcatt cccgccacat cgagacgctc gcggcggaaa ccgcaaggct cggcctcggc
      241 ggccgggtga cgggctcgca tctgacctcg atgcacagca tggacaacta ctacgtctcg
      301 aagctcctgc cgctcatcgc cgaggcgggt gtggcggcga tcgccaatcc cttgatcaac
      361 atcgtcatcc agggccgcca cgacacctac cccaagcgcc gcggcctcac ccgaatcgcc
      421 gagctgcagg ccgccggcgt gccggtcgcg ttcggccacg actgcgtcat ggacccctgg
      481 tattcgctcg gctcgggcga catgctcgag gtggcgcaca tggcggtgca tgccgcccag
      541 ctcaccagcc gcgaggcgat gcgggccgcg ttcgccgcgg tgacgagcgt gccggccggc
      601 atcctcgggc tcgacggcta cggcctcgag cccggctgcc acgccgacct cgtcctgctc
      661 gacgccgagg acccgatcga ggcgatccgc ctgaaggcga cacggctcgt cgtcgtccgt
      721 cgcggccgga tcgtcgccga gacgccggcc gcggtcagcc ggcttcatct ccccgaccgg
      781 ccgccgagcg tctcggcgcg ccgctcggcg cctgaatcga gcgcccacta accgacgctt
      841 aactctcttg tggcagcttc gaggctgtct ctcgggagcg gttggatgtt gagctcgagg
      901 cctctagacg aaatgtccct ggcgatggtc aaccggcggg ccctcaatcg gttcgcctgg
      961 atccttggct gcggactcgc tctggccctt tccctgccga gcattctctt cgccgcgacc
     1021 ttcggctcgt tcaccgggat cggcgccggg atcgttgcaa cggtggcact cctcgcccgg
     1081 gaagagcccc tagcacccca tctcacgcgc tgggacatcg ctgccgccct ctatgcggcg
     1141 agcctgttcg ccgggctctt cgtcgatgtc gaaggcgtgc ggcactacct cctgatgcag
     1201 cagcacgggt ttccctgaac gaagcagcga ggcgatgtcg atgcacgacc tcgcctcgcc
     1261 gaatgctcac cgtgctgtgc ggcagcggca aggtgtcgag ggcagattcc atttggcgat
     1321 ttcgacgaac gcgggcgcaa gctttgcgcg cagcgaatga agcctgcctc cgacaagggc
     1381 aaatctccca aggatcattg gttcctcgtg agttcccgct gagcgcgatg cttgccgata
     1441 ctcgcagtca cgccggcgcg tccaagcgcc gtcggatcgg tcgtagtttt cgcttccgca
     1501 agcccgactc cgccggtttt cgcgacattg cccgcggcag cgcgcatcgc tttttcatat
     1561 ttgcgcttgg cgcttcttcc aaactctgcc atctacgccg atcagtgcgc cgctcgcccc
     1621 ggcaatgaca atcaataaga cgaagagcaa cggcagactg atgctcgcct tcaaccgaac
     1681 cgatctcgaa cgcctgttct ccgaagcgat ctggctcaag gccgagcatc tcgtcgggga
     1741 cggggcggtg gtcgatgtga gcgtcgagcg ggacggcaag tcgatcaccg gccagatcaa
     1801 gggcggtcgg cggacgcctt ttctcacgcg gatcaagatc gccaatggcc ggggcggccg
     1861 ggttcgactg tcgagcacct gctcctgctt ctatccagaa tgcgagcacg ccgccgccac
     1921 cctgctcggc ctgctcgacc agtcgaccac gctcgacccc gaggacggct cggtggtcgt
     1981 cgaagccgag ctcgagagct gggttgccgc ggtcaacgcc gcgacccgca atggccatgc
     2041 cgatgcggcg gacgtcgtcc tctatctgct cgagccggcc cagcgctcct ggcgtgacgg
     2101 cagcctcgcg cagccggtgc tgatcaacac cgcccgggcc aagcggttgc gctcgaacgc
     2161 ctatggccgc gagaacccga ttgccatcgc caatctcctc gccgatcctg cccccggcta
     2221 tgtcggcctg caggaccagg tgatcgggcg cctgctcggc ggcggcaccg gccagagccg
     2281 ccggctcggc agccgggccg atggcgaggc cctgatccgc gtgctggaga ccggccgctg
     2341 ccactggcgc aacgccgcgg gcccgccgct ccgcttcgtc gccgagccgc ggcgcggcgg
     2401 cttcttctgg cggttcgaca gcgagggtca gcagcatctg gtctgcgagg tcgacgaccg
     2461 gcacgacgac ctcgtggtcg tggcgctcgg cgagccctgg tacatcgatc tcgccacgag
     2521 cgaggtcggc cgggtcgaga ccggtgtcga tgccgcgctt gccggccatc tcgtcaacgc
     2581 gccggcgatc ccggcggcgg ccgccaccat cgtccgccag cggctgatcc cgagcggcga
     2641 gcgcctgccg ctgcccgagc ctttgaagaa gcgcgagcgg ctcgagacca agccgcagcc
     2701 gatcctgctg ctgcactgcc ccagcctcgt cgtgcatcgc ggcacgggct ggcggcgcga
     2761 ggagcaggag gtcgactgcc cgctcgcgcg ggtcgccttc gactatgccg gagccgagat
     2821 cggctggcag gatggccggc ccgagatcaa ccacgtccag aacaaccgtc tcctggtcct
     2881 gccgcgcgac acgattgccg aggtgcaggc gatcgagcgg ctgaacacgc tgggcctgca
     2941 gccgctgggt cccaccgggc tcggccgttt cgcgccggag ccgctgcgcc aggatttcac
     3001 cttcgaggag gacgaggacg acgacgtctc ggtccgctgg gtcgagttca accatatcga
     3061 cgtgccgaag ctgcgcggcg agggctggcg cgtcgtctat gacgagacct atcccttcca
     3121 ggtcatcgac aagccggttt cctggcagat cgatctcaac gattcgggca tcgactggtt
     3181 cgagctcgat gtcggaatcg agctcgacgg cgagcggatt ccgctgctgc cggtcctgct
     3241 cgatctcttc cagcgctcgc ccgacgacat gacgccggcg gcgctcgccg cctatggcga
     3301 ggagcccgtc tacggcacgc tgccggatgg ccgcctcctg ccgatcccgg cggctcgcct
     3361 gatggccatg ctggaggcgc tctacgagca gttcgtcaac aaccgggtcg atccgcaggg
     3421 gcggctgcgc ctggcgccgg ccgaggcgtc caggctcacg gtcatcgacc ggacgatgac
     3481 cgaggacgag gtcgtctgga ccggcggcga gcgcatcctg cgcctcgccc gccagctcgg
     3541 cgacaacgac cgcattccga cggtggagcc gcccagggcc ctgcaggcca agcttcgccc
     3601 ctaccagcag cagggcctga gctggctgca gttcctggcg tcggtcggcc tctcgggcgt
     3661 gctcgccgac gacatgggcc tcggcaagac cctgcagacc ctggcccaca tcctggccga
     3721 gaaggaaggc ggcaggctga ccaggccggc gctgatcgtc gcgcccacct cgctcattcc
     3781 cacctggcgg agcgaggcaa ggcgcttcgc gccgtcgctc aacatgctga ccctgcacgg
     3841 caacaaccgc ggcgatctct tccgccgcat cgagcgcagc gacatcgtgc tgaccagcta
     3901 cgccctgatg ctgcgcgatc gcgacgagct ggtgcgccgc acctaccgga tggtcgtgct
     3961 ggacgaggcg caggccatca agaacccggc gaccaagctc gccaaagtgt cgtgcgagct
     4021 ccaggcggac cacaagatcg cgctctccgg cacgcccatg gagaaccatc tgggcgagct
     4081 ctggtcggtc ttccatttcc tgatgccggg gttcctcggc gaccgcgagg ccttccggcg
     4141 gatcttccgc cagcgcatcg agaaggacgg cgacgccgac cgccaggcgc tgctggtgca
     4201 gcgcgtccgc cccttccttt tgcgccgcac caaggaggag gtggcgtccg agctgccgcc
     4261 gaagagcgag ttcatccgcg agatcgagct ctcggacgac cagcgcgatc tctacgagag
     4321 cgtgcgcctc gccatgcatg cccgggtgcg cgaggagatc gagcagcggg gcctggcgcg
     4381 ctccaacatc gccattctcg aggccctgct gaagctccgg cagatctgct gcgatccccg
     4441 gctcctgaag aagcgcgacg ggccagcgcc agccagcgcc aagtacgaga tgctgatgag
     4501 catgctcgag ggcatggtcg agaccgggcg gcggatcatc gtcttctcgc agttcgtcga
     4561 gatgctggag ctgatcgtcg aggggctcga ggagcagggg atcggctaca ccatcctcac
     4621 cggccggacc cgcgatcgcg aggcgccggt gaagaagttc cagaacggcg aggtgccggt
     4681 cttcctgatc agcctcaagg ccggcggcac cgggctgacg ctgacccggg ccgacaccgt
     4741 catccactac gacccctggt ggaaccccgc ggtcgaggcg caggccaccg accgggcgca
     4801 tcgcatcggc caggacaaga ccgtcttcgt ctacaagctc gtcgccagcg gcaccgtcga
     4861 ggagaagatg gtggagctgc aggagcgcaa gcgcagcctc gtcgagggcg tgctcggcgg
     4921 ggtcaagggc ggcggcgtcg gcctctccga gaccgacatc gaggcgctct tcgcgcccct
     4981 gcccgactga gccacgctct cagggcgacc gcgtccggac gccgaactcc cgcccggctt
     5041 cgtgcagcaa tgcttcgaca tagggtgcca gggagcggtt gacggcgagg cgccagcggt
     5101 cggcggccag ccggtccagc acgatctcgg ccttgcccag catggtgcgc gtggcggcat
     5161 cgacggcgaa gctgctgtcg tgcaggtcga gcgggcaggc agcggccagc gcctggcgga
     5221 ccatcggccc ggagagctcc agcgcggtga ggcgggccga gacatcgacc agggcgtggt
     5281 ggtggtcggc gagcgccggc cgaaggcggt cgacgaggcc ggccaccgtg gtacgcgcca
     5341 cgagcatgag aaattcgtcg gggccgaggc gcagggtccg gtcgtcgacc tcgcctgccg
     5401 cccggttgat cgcggcgggc cagggccggc cgagcacgtc ggctgccagc ttcgccacct
     5461 cgtcggtcgc cctgagcgtc agcttgccga gctcggggcg gggccgcagc tcgatgcctg
     5521 ccgctttctc gtggtcagcc atggaggcgg ctcccctccg gatcgatgaa gaggggcttc
     5581 gtcaccaggg cggcgatcga ctggcctgac ggcatcggga cgtggagcgt ctgcccgtgg
     5641 cggccgtggc caccttcgac cagggccagg gcgatcgagc ggccgagatt ggggctgtgg
     5701 taggccgagg tgacgtggcc gaccatccgc gccggcacgg cggggttcgc agcctcggtg
     5761 agctgggcgc cctcctcgag gacgcgctcg ggctccgcgg tgagcaggcc cacgagctgg
     5821 cggcgatcgg ggtgctggag atcctcccgg gtgaggccgc gcttgccgat gaagtcgccc
     5881 ttcttcttcg cgatgatcca gtccatgccg aggtccagcg gcgtcaccgt gccgtcggtc
     5941 tcctggccca cgatgatgaa gcccttctcg gcgcgcagga catgcatggc ctccgttccg
     6001 tagagctcga catcgtgtgc gcggcccgcc tcgagcaggg cttcgaggat ctcggctccc
     6061 cggtcggccg ggacgttgac ctcgaaggcg agctcgcccg tgaagctgat gcggaagatg
     6121 cggcccgcga tgccggcgat gcgggcggga cgccaggcca tgtgggggaa ggcatcggcg
     6181 gcgagatcgc atccctcgac caggggggcc agcacgtcgc gcgcctttgg gccgttgatg
     6241 gcgatggcgg cccattcctc ggtgacagag gtgagataga cctcgaggcc gggccactcg
     6301 gtctgcagca ggtcctcgag ccaggcgagc acgcgggcgg cgccgcccgt ggtcgtcgtc
     6361 atgtggaagt gccggtcgtc gaggcaggcg gtgacgccgt cgtccatgac gaagccgtcg
     6421 tcgccgagca tcaggccgta gcggcacatg ccgggggcga gcttcgacca ggcgttggtg
     6481 tagacgcggt cgaggaaggt tcgggcgtct ttgccctgca cgtcgatctt gccgagcgtg
     6541 gaggcatcga gcaggccggc ggtgcgccgc accgccgcgg tctcgcgggc gacggcggca
     6601 tgcatgtcct cgcccggctt cgggaagtac caggcgcgct tccaggtgcc gacatcctcg
     6661 aagaccgccc ccttcgccgc ggcgatctcg tgcatcggag tccggcggac gacgtcgaag
     6721 agcgcacccc ggctgtggcc gacgatggcg ccgaaggcga gcggcacgta gggcgggcgg
     6781 aaagtcgtcg tgccgacgtc ggcgaccggc ttcgccagct tctccgcgac cagcgccagg
     6841 gcgttgacgt tcgaggtctt gccctggtcg gtgcccatgc cggtcgtggt gtagcgcttg
     6901 acgtgctcga tcgagcggaa gccttcggtc agcgccaggc ccagatcctt cgccgtcacg
     6961 tcgttctgga agtcgacgaa ggccttggcc ctgacggacg gccggtcggc cggcaccgcc
     7021 cagagcgggc ggagcggggc aggatcgccg tcgaagccgc tgaagtcgcc ggcgcaggcg
     7081 ccgacgcagc tcgtcgcctg cacggcctcg cccggcacga aggcctgcag gcgctcgtcg
     7141 aagcggagcc ggccgcggct ctgcgagaaa agcgagacat tggggttcca gccgcccgag
     7201 acgagcagga gatcgcaggc catgggctcg cccagatggc ctgcgaggcc gccatcgcca
     7261 gcgagccgcg ccacggccgc ctgcgtgatg cgcctgcgtc cctccgtgcc ggcgacgacg
     7321 gcattggtga cgacacggat gcccgcggcc cgggccgcgg cgacatgggc gccctcgggg
     7381 ttggcccggg cgtcgacgat gcaggcgatg ccgacacctc tggcggcgag atcgagggcc
     7441 gcgacatagg ccgtgtcgtt gttggtgaag acgacggccc gcgtgccggg gcggacggcg
     7501 aagcggttga gataggcgcg cgcggccgag gccagcatga cgcccggacg gtcgttgccg
     7561 gtaaagacga tgggccgctc gatggcgccg gtggcgagca ccacctcacg ggcgcgaatc
     7621 cgccagaggc gctggcgcgg caggtccggg tcgggatcgg cgagatggtc ggtgacccgc
     7681 tcgagggcca cgacatagcc gtggtcgtag tagccggtga ccgtcgtccg ggtcaggagc
     7741 gtcacctcgg gcaatgtcgc gagctcggtg agccgcccgg cgagcgccaa cggcacgtcc
     7801 gggcgtgcca gccagccgcc gccgagctcg ctctgctcgt cggcgaggat gacgcgcttg
     7861 ccggcacgcc cggccatgag ggccgcatcc agcccggccg ggccgccgcc gacgacgagc
     7921 acgtcgcaat gcgcgtggcg gtggaggtag cggtcgggat ctggttcggt cggggccaca
     7981 cccagcccgg ccgcggcccg aatgcgcggc tcgtagacgc tcttccagaa ggaggccggc
     8041 cacttgaagg tcttgtagta gaagccggcg ggcaggagcg gcgagaggag gtcgttgatg
     8101 gcgccgatgt cgaaaccgag ggaaggccag tggttctggc tgcgcgcatc cagcccgtcg
     8161 atgagctcga ccacggtggc ccgcatgttg gggtcgcttc gcccgccgcc ccgatcgagc
     8221 tggaggagcg cgttgggctc ctcggggccc gcggtcatga tgccgcgcgg gcgatgatac
     8281 ttgaagctgc ggccgacgag atggatgccg ttggcgagga gcgccgaggc gagcgtgtcg
     8341 cccgcgaaac cctgcatttc catcccgttg aagcggaacc gcagcggccg ctcacggtcg
     8401 atccgcccac cttcggcgag gcgaaagttc tctgccatca cgtccggccc aggtcgggcg
     8461 gcgggctgcc catgggatag accgccagga tctcgtcgct caccgtgtcg cgcgccacgt
     8521 tgaaccagcg ctggcagccg tggatgtggt tccagcgctc gagatgcagg cctttcgggc
     8581 tccggcgcag atagagatag tcgcgccagg ccgcgtcgct cgtctcctcg ggcgaggccg
     8641 gccgggcgat atgggcctcg ccgccgcagc ggaactcgat ctcggcccgg gggccgcaat
     8701 gggggcagtc gatcagcagc atcgggctcg tcctagtgcg ccacggcggc ggcggcggcc
     8761 tcgtcgatca ggcggccggt cctgaagcga tcgagggtga agggggcgtt gatcttgtgc
     8821 ggctcgccgg tggccagcgt gtgggcgaag acgtgggccg agccggtcgt cgccttgaag
     8881 ccgcccgtgc cccagccgca attgacgaag aggccctcca ccggggtctt gccgatgatc
     8941 gggctgcggt cgggggtgac gtcgacgatg ccgccccact gccggagcat ggccatgcgc
     9001 gagaacatcg ggaagagctc gaggatggct tcgagcgtgt gctcgaggat ctggatgccg
     9061 ccgcgctggc tgtagctcac atactggtcc gtgccggcgc cgatgacgag ctcgcccttg
     9121 tcggactggc tgatataggc gtgcacgctg ttggacatga ccacgcaggg aaagacgggc
     9181 ttcaccggct ccgagacgag cgcctgcagc gggtaggatt cgagcggcat gcgcacgccc
     9241 gccatgccca tgacgaccgg cgtgctgccg gccgtgacca cgccgacccg cctggcggcg
     9301 atgaagcctt tcgcggtctc gaccccgacc acgcggtcgc cctcgcggcg gatgccggtg
     9361 accgggcagt tctggatgat gtcgacgccc ctggcatccg caccgcgcgc atagccccag
     9421 gcgaccgcgt cgtgccgtgc cgtgccgccg cgccgttgca gggcggcgcc caccacgggg
     9481 tagcgggcat ggcggtcgat cgacaagggc gggcagaagg cttgcgccgc ctcgggtgtg
     9541 agccactcgt tgtcgacgcc gttcaggcga ttggcgtgga tgtgccgctt cagcacctgc
     9601 acgtcgtggg tgttgtgcgc cagcatcatg acgccgcgct ggctgaacat gacgttgtag
     9661 ttcagctcgg ccgagagccc ttcccacaag cggaccgagt ggtcgtagat ggcggcgctc
     9721 tcgtcgtaga gatagttgga gcggatgatc gtggtgttgc ggccggtatt gccgccgccg
     9781 agccagccct tctcgatcac ggcgacattg gtgatgccgt gctccttggc gagatagtag
     9841 gccgtgccca gcccgtggcc gccggcaccg atgatgacga tgtcgtattc ggggcgcggc
     9901 tcggggctcc gccaggcttg gggccagttg cggtgaccgg tgagcgcgtt ccgcgccagc
     9961 gagacgatcg agtagcgttg catcggcccc tgccggctcc cctctgcccg tcgacgacct
    10021 agcccatgac gcggaccggg ctttgcccgg cgcgacccct tcatgcgccg gcgcggcggg
    10081 cggcggcggc acgccgctca ttaaccacga ttccggcggg tccgccaccg agggacggcg
    10141 ctcgccaggg ccgaacaccg ttcgcctgcg gcaagagcac gcggaacgcg ctcgccagct
    10201 tagcagttga tttccgttgg gccgacttcg cggcttcact ctgcacacgg agaggaaaca
    10261 ccatggaacc gacgctctat ctcttcttca agggcaactg cctggaagcg atgacccact
    10321 atgccgagac gctcggcggc aaggtcggcg acgtcttccg caacggcgac gcgcccgatc
    10381 cggagagccg catgcccggc ggcgacgaca tggtcatgaa catgagcatg cagctcggca
    10441 acgcgaccgt catggcgtcg gacaacagcg acgggatgta cgagaagccg cggggcttcg
    10501 ccgtgtccat cgccccgcca tcgctcgccg agttcgagcg catccatgcg gcgctggcgg
    10561 aggatgcgca gtccgtggcg atggcgcccg gcgagacctt ctgggcggag cggttctcca
    10621 tgttcaccga ccgctacggc acgccctgga tgctcaatta cgagggcgac aaagcgcaaa
    10681 gctgagggag actgccgatg accgagctcg tcacctgcct ttggttcgac cacggtgaag
    10741 cgaggaaagc ggcggaattc tatgccgcca cctttcccga cagccaggtc gggcgcgtga
    10801 atgccgcgcc cggcgacttt cccggcgggc aggagggcag cgagctgacc gtcgagttca
    10861 cggtgctcgg ccgcaagttc gtcggcctca atggcgggcc gagcttcacg ccggacgagg
    10921 cgatcagctt catggtcgtc accgacgacc aggaggagac cgaccgctac tggaacgcga
    10981 tcgtcggcaa tggcggggcg gagagcgcct gcggctggtg caaggaccgc tggggcttct
    11041 cctggcagat cacgccgcgg ctgctgctcg agctgacgac gagtgccgat cgggcgaagg
    11101 ccaggcgcgc tttcgacgcg atgatgagga tgagaaagat cgacctcgcg gcgctcgagg
    11161 ccgcggtggc gaagagctga ggcgacagca gcacccaagc gaaagggagc gaccatcatg
    11221 gcctacgtcg acggcttcgt gatcgcggtg ccgaccgcca accggcggag gttcatcgat
    11281 cacgccaaca aggcgaacac cgtgttcatg gagctcggcg ccacgcgcgt cctcgaatgc
    11341 tggggcgacg acgtgccgaa gggcgagacc acggatttcc gcatggcggt caaggcgaag
    11401 gaggacgaga cggtggtctt ctcctgggtc gaatggccgg acaaggcgac ccgcgacgcc
    11461 gccatgggcc ggatgcggga gctgatgaag accgacgacc ggtgcaatcc ggagaagaac
    11521 ccgatgccgt tcgacggcaa gcggatgatc ttcggcggct tcgagcccgt ggtggagctc
    11581 tgaggcaggg cggctgcgac cgccaagggt cgcagcgggg gtcggcgcgc gcctgcgttg
    11641 tggttggctg gaaaaaactg tgttaggccg tgccgatgtg agcgaatggt gacggtcgat
    11701 gcaggtggtg acggcggcgg gcgcggcggt cgataccgag acggttgcgt tctttctcgt
    11761 ccccgatttc tccatgatct cgttcgcgag cgccgtcgag ccgctgcgca tcgccaatcg
    11821 gctgtccggc cgcagcctgt accgctggct gctgctgtcc aaggacggtg gcccggtgcg
    11881 cggcagcaac ggcatcagcg tcaatgtcga catggcgatg gaccggatac aggcgagctt
    11941 cggccagtcc ctgccgacga tctgcctcgc ctccggcctg cacggtgagc gctacgccga
    12001 caaggacgtc ttcgcctggc tcaggcgcat ggaccggcac ggggcagtga tcggggcact
    12061 ctgcacgggt gcccacatcc tcgccaaggc cgggctgatc acggaccacc gctgcaccat
    12121 tcactgggag aacctgcccg ggttcgtcga ggccttcccg gatatcgacg tcgccgcgga
    12181 cctctacgag atcgaccgca accgcttcac ctgctcgggc ggcaccgcac cgctcgacct
    12241 gatgctgagc cggatcgccg cggcccatgg cgaggagctc gccaccaagg tcagcgagca
    12301 gtgcctgctc gaccgcatcc gcggcccgca tgaccgccag cgcatgccga tccgcatccg
    12361 gctcggcgtg caccacccca agctgatcaa cgccatcgag ctgatggagg cgaacatcga
    12421 ggagccgctg ccgcaggagc agctcgccca ctatatcggc ctctcccgcc gccagctcga
    12481 gcggctgttc cgccgccaca tgggccgcac gccagcccag tactatctcg agctcaggct
    12541 cgagcgggcg aggcacctgc tctaccagac cgacctgccg atcatgagca tcgcctgcgc
    12601 ctgcggcttc gtctcggcct cgcacttctc cacctgctac cggcagatgt tcggtcgcac
    12661 gccccgggcc gagcgcaacg tcgcggcatg agtcctcgag gttgggctca ccgaagattt
    12721 cctgacatct tcgattgatt gtagccggcg ctacagccgc ccgggccctt cgtaacgcat
    12781 gatcggctca catcgaggca gtcaggcacg aggccgatca tgcgttgcaa tgaaccacct
    12841 cccgaataca ccgattccct gcacccgttg gtgttcgact cgctgctgcc cgcggcgggg
    12901 tccagcgttg tcctggtgat cattgcgttc ctggggctcg cttttctctg agtgaccgcg
    12961 tcaggccttg acgccggcac cgccgagcgg ctcggcctcc gggtcgagcg gccagcgcgg
    13021 gcgggcggcc acgtcgaggc cgtcggtcag gccgagcgcc agccgctcga tccctgccca
    13081 ggccaccatc accccattgt cgccgcagag ggccggtggc gggatgacga gcccggcacc
    13141 atgcgccaca gccagctcct ggagccgggc gcggatgtgg cggttggcgg cgacaccacc
    13201 ggcgaccacg agttggcggg cggcggcggt tcctgcctgc cgctgcaagg ctctcgccgt
    13261 tcgatcggca aggcagtcgg cgacggccgc ctcgaagctg gcgcagagat cggcgacgaa
    13321 ggcgtcgtcg acagcgtcgg ctgcgaggat cacctggcga accgccgtct tcaggcccga
    13381 gaacgagaag ttgcagtgcg gctgagcgta gagcggccgc ggcaggcgaa agcggtgggg
    13441 gtcgccgccc tgcgcggccg cttccacgag cgggccgccc ggatggccga gcccgagcat
    13501 cttcgccacc ttgtcgaagg cctcgcccac cgcatcgtcg atggtgctgc cgagccgcgt
    13561 gtagcggccg acaccctcga cgagctgaag ctggcaatgc ccgcccgaga cgaggaggag
    13621 cgtgtagggg aaggcgaggc cgtgggtcag ccgggcggtc agggcatgcg cttccaggtg
    13681 attgacggcg accagcggca ggccggtggc gaggcagatc gccttggcgg tgacgatccc
    13741 caccatcagg ccgccgatca ggccggggcc ggcggtcacc gcgacggcgt cgagcccggc
    13801 gtagtcgagg cccgcctcgg ccatcgttcg ctcgatcagc cggtcgaggc gcaggagatg
    13861 ggcgcgggcc gcgatctccg gcaccacgcc gccatagagc ttgtgctcgt cctcctggct
    13921 gaacagcgac tgggcgagca cccggcgctc gccatcgacg atggcgacgc cggtttcgtc
    13981 gcaggatgtc tcgatgccga ggacgatcgc cactggcggc agggctccgg tctgctaggt
    14041 caggtctgga gatgatcggt taggtgatgg atggaatgga caagggccgc aagctgaaga
    14101 tcgggacccg aggctcgccc ctggcgctgg cccaggcgga gctgacccgc caggcgctgg
    14161 ccgacgcgcg gccggcagcg ctcgcggtgc cgctcgaggc gacggagatc cgggtcatca
    14221 ggacgaccgg cgaccacatc ctcgatcggc cgctcgccga ggtgggcggc aaggggctct
    14281 tcaccaagga gatcgaggag gcgttgctgg cgggcgagat cgatctcgcg gtgcattcca
    14341 tgaaggacat gccgacccgc ctgccggagg gcctggtcat cggcgccatg ctgccgcgcg
    14401 aggaccccag ggatgcgctg atcctgggcc cggagctggc cggccgttgc cacggcatcg
    14461 ccgggctgcc gcaaggggcg cggatcggca cggcgtcgct gcggcgcatg gcgcagctca
    14521 aggcggcgcg gcccgacctc gtcgtcgaga cgctcagggg ctcggtcggc accaggctcg
    14581 gccggatcgc ggagggccgc ttcgccggca cgctcctcgc ccttgccggg ctgcagcgcc
    14641 tcggcatggc cgaccgggcg agcaccgtcc tcggcatccg ggagatgctg ccggccgtgg
    14701 cgcagggggc catcggcatc gagtgccggg cggacgacgc gtggctcgtg gagcgcctcg
    14761 ctgcgatcgg ctgccgggcg acggccgcct gcgtcgatgc cgagcgcgcg gcgctggccg
    14821 ccctcgacgg ctcctgccgc acgcccatcg ccggccttgc gggaatcgac ggcacggagc
    14881 tgctgctcca ggtgctgatc gcccggccgg atggcagcac ggtgcatcgc gagacgggca
    14941 aggcccatgt catggacggt gtcgccctcg gcgaggccct cggccgcaag ctccgcgagc
    15001 gcggcggccc cgacttcttt cgggattgag cccgggccat gcacgtcctg gtgacgcgac
    15061 cggagccgca ggccacgcgg acggccgagt tgctggccga gcgcggccat cggacgcacg
    15121 ccgcaccact ggccgtgatc gagcccctcg aggccgagct gccagcggca gtagcggtgg
    15181 acgccgtgct cctgaccagc gccaatgcgg tgccggcgct gcaagcctat ctccacctgc
    15241 cggtctacac ggtcggcgat gcgacggcgg cggcggcacg ggcagccggc gccgggcagg
    15301 tggtgtccgc cgatggcgac tgggtgcgcc tcgccgccct gctccggggt ccagcgggcc
    15361 caccggccgg gtcgcggctc ctccacctct ccggcaccgt catcgccggc gatctcgcgg
    15421 gcgccgtgcg ggccgcgggc ttcgcctgcg accgccgcac cgtctacacc attcgacccg
    15481 caacgacgct accgccggaa ctgctgcgcc tgctcgccgt tggcacactg gacgcggcgc
    15541 tcttcttctc gccgctgcat gcgcggatct ggtgccggct ggtggcaaag gcaaccatgg
    15601 aagcggaact ggccgggatt cgggcggttt gcctgagcga ggcggtggca caaccattga
    15661 gggaactggg gtggcgaagt gtcgaagtgg cggctgcgcc gaccctgcct gcgctgatcg
    15721 actgccttga aggccccgga tgaagatggt agcccatgct cgggactagg acgtcggcga
    15781 gcctgcgcct ctcgggatgg cagccggccc attcgacgca cgcgcgtcgc tgtcggacaa
    15841 ctggggagag catcgccgat gtcgcgagga cgatcgcgca acccgtcgca gggacagccg
    15901 aaggctcagg actcgcggcc ggaccacgca gcaaagccgg ctgacccgca gggcgcgtcg
    15961 gcaacgcccg ccggccagac ggagccgagc aaaccgcccc aggccgctgc gccgccgaag
    16021 gatttctccg gcccggagtc agcgaagccg gtcgacgctg cacgcccgac gacgccgccg
    16081 agccccccct cgcctgccgc ttcatccgcc agcccgggtg acaggcccac gggcggcccg
    16141 attgccgaca agggtccgac cccgggcgat ccggccgcgc cggctgcttc cgccaggccc
    16201 ggcgaagcgc cgaggagccc ggcgccttcg tcacctgcgc cggcgtcggg caagcccggc
    16261 gaaggggtcg aacggccagc gagtgccgct gccgcgccgg caggcggcgc gccccggacg
    16321 ccgcccaccg gcgtcggcac gacgaaatcg gcgccggcgg caccaccgcc cccggctcca
    16381 gcgccggcga agggcggcgg gggctatgtc gccggaatcg tcggcggcat catcggcgcc
    16441 gttctcgtcg gtgtgggcct gccgctggtg cagggcccgc cgccgctggc gcccgaggcc
    16501 gcgcagcgcg tcgcggcgct ggagtcgagc gctacgaccc tggacgagca gcttgccgcg
    16561 gctaccgagc aattgagcac cgccaacgag cagatcacca atctgaaccg gctgtcggcg
    16621 gagctggaga gcacggtcga aagcgccgtg gccgcggctg tcgagcgcat tccgtcacgc
    16681 gatggtgccg ccatcgccac ggtcggagag cgactggacg aggtttcgtc gaaggtcgac
    16741 gagctggtcg cttcggccgc cgccgcgacc gacccggcac ctctgctggc cgcccaggcg
    16801 caacggctcg aggcgctgca ggcagcgctg accagcctgg acaaccgcct ggccagcgag
    16861 gtgctgcggc tcgaggcggc gcggaccgag acggcaacgg gcctccaggc gtcgatcgac
    16921 gagaccgctg cagcggtgga gacggcgcgc caggcagcta aggacgagct cgaagccgct
    16981 cgcacgaccc tcgccgaggg catcgccgcc ttcgagaccg atctcgaagc ggtccgtgcc
    17041 gacatcgcct cgctcgaggc gacggttgcg gacatgcagc tcgcccgcaa ccgggccgca
    17101 gccgcggcgc tcctggtgcg cgacatcgac cattcgatca ccgccggcga gccctttgcc
    17161 gagcccctgg accgcctcca ggcgatgggc accggcaatg ccgagctcga ggccaccctc
    17221 gccagcctcg cgccggcggc ggcttccggc gtgccgacga tcgacgagct gcggcagggc
    17281 ctcgtcgagc tcgaggcgcg gcaagcggca ccgccgatcg gtgggtccga gctgctcggc
    17341 ggcacggtcg gcaacatcct cgggctggtc gaggtccgcg accgcgacac cgacggcatg
    17401 gcggccaccg gcctgctcgg cgaggcggac gccgccctgc gccggggtga tctcgctgcg
    17461 gccatgagcc atgtcgaggc gaccagggac atgccggacg gccccgatcc ggccgcgatc
    17521 gacgcctggc tggaacaggc tcgcgcccgt gcgaccgcgg tcgccgcgct ggcggcgctc
    17581 gacgcgcaga ttcgcgactt gctcaccgca acggtcaact gacggcgacg cggcaccgag
    17641 gtcattcgat catgctcaga ctcatcgtgt ttctcatcgt tgccgcagcg ctcgcctggg
    17701 tcgccgtctg gctcgccaac agcccgggcg aggtggtgct gcgctgggag gcgaccgaca
    17761 ccgtcctgcc ggtcggcatc gcggtcgcca tcgtcgccgc catctcggcc gccgcgatcg
    17821 tcgtctacga ggtctggcgc tggctcgccc agctgccgcg tcgcgtgcgc tccaaccgcc
    17881 agcagaagcg ccagcttcgg ggctaccagg agctcagcca cggcctgatc gccgccgccg
    17941 ccggcgacgt cacgggggcc cgtgcgcata cgcggcaggc cgagaagctg ctgcaggcca
    18001 atgcggcgac cctgctgctc tcggcccaga ccgcccagct cgagggcaag gaggacgtgg
    18061 cccagctcaa gttcaaccag atgctcaagc gcccccagac ggagttcctg ggaatccgcg
    18121 gcctgctcgc ggatgcggtc aagcgcggcg accagaagga ggcgctggcg ctggcgcggc
    18181 gggcctacga gcgcagcccc aacacgtcat gggtgctgac cacctatttc gacctcctga
    18241 cgcgctccgg caaatgggcc gacgcgctca agctggtcaa cgacctcgcc cgcgagaagc
    18301 tggtgagcgg ccccgaagcg acgcgccgcc gggcgctgat gaagcacatg ctggcggtcg
    18361 aggcggtcga cgagaagcgc tacgccgagg ccctggcgca ggcgcgcaag gcgaccggcc
    18421 tcgcccccac cttcgcgcct gccgccgtga ccgcagccca ggcggccaat ggccagggca
    18481 agctcaggct cgcccgacgg ctgctcgagg actgctggcg gctggagccg catccggagc
    18541 tcgccagcgc ctatgccgca ctcgtcccgg acgagaccgc cgccgagcgg ctgcggcgcc
    18601 tcggccggct ggagagcctc gtgccgaacc atctgaccac ccagctcctc atggccgagc
    18661 tcgccatgaa cgcccgccag tgggacacgg ccaagcgcca tctcgacaag gcgctcggcc
    18721 tggagcccac ggccggcgcc taccggctct atgccgagta cgagcgggcc tcgggcgggg
    18781 gcgacgccaa ggcgcgcgac tggctggcca aggccgccga cgcgccggcc gacaaggtct
    18841 gggtctgcga ggacacgggc gaggtcctgg ccgaatggca gctcttcggc ccgtccggcc
    18901 ggttcgactc ggtgcactgg gacagcccgc ccaaggtggc gccaatgatc cgcgaccacc
    18961 gcccgccggt cgtcctggtc cacgaccagc gcgccgtcgg cgagcccgcc gagcccgtga
    19021 ccgaggtcga cgatgtgaag aaagagaagg acaaggcacc gggcacgtcg aagaccggtg
    19081 ccaccgagca gggcctgaag gcgccgccac cgatcgaggc cacggtgaag gccgccggca
    19141 gcgtcggctg accgcccttc gcctgccgcc ttttcccttg ccccgcaccg acccgccggg
    19201 ctatctcccc accgcacgcc gcagtagctc agctggtaga gcacatcatt cgtaatgatg
    19261 gggtcggggg ttcgagtccc tcctgcggca ccaaatttcc ccgaaaattc aataacttat
    19321 agttgaccac caaagactcg gacaccgcgc ttgaaaacgt gtccgcacag tgtccgcaat
    19381 tcgggatggg cggccggact gttccacgaa ccgccagcgg ccagttcaag tccgcacgat
    19441 cacatcgcca tcggcagcgc cgacatacct ggcgcgacgc caacggacga cggatcaccc
    19501 tcggcgatct tcaaaagcat tcctgatggt gaccgctgca acgcgattga tctccgcttc
    19561 gatcggacgt tttggctcta gcgtttgcac catcgcgagc gcttctgaga ttgcgtcatg
    19621 caactgttcg gtcgtcatga ttccgcgctc ttggagcact cgacaaagct gttgaatgca
    19681 cgccatcgcg gccaaccccg tgccgcgcgc ggtgatcaat tcgtttcgtt cttcttcggt
    19741 catgaacgcg tccccccaca tctcggcatt ttgccaagat tgcggcggac agcggcgccc
    19801 gtctagtcgg aaatggccag tcgactaagc tcggccgccg cggcgttgac cgcgacccgg
    19861 ccgtgctcgg cgggctaccg gaggagcgcg gccacgccgg cgctcggcgc gacgacgggc
    19921 cgcctgccca cgtagggcga cggtgccgcg gcccggccgg tgaccgcccc caggatgccc
    19981 agggcctccc tgagggcgtc gcgggcggcg gcgagagcct tcgggtcaat gtcgtcgggt
    20041 agggccgccc attcgtccag cgcgtcctga gcgagcttga aggccgcttc ccgggccgga
    20101 tcgccgtcct cgagccgagg gcgggtgacg acaaccatgt tgctgcccgg ccgcgccctg
    20161 ccggtgagcg ccagctcgcg atagtcggtc tgcctcactg cagcggcccg cgatcgatgt
    20221 cgtcggccca gccgaccagg ctgagcagct tggccacctc ggccggctcg aggcccgctg
    20281 ccttggcgct cgccatggct tcgaccatgc cgctgaaggc tcgcgccttg ccgccggcat
    20341 cccaggcttg cagcggcctc atgacgtcga tcttgaccgg gccgccgagc ttggccccgg
    20401 cttcctcggc gatcagctcg gcgatgggct ggagggtcca cccggccagg tggcgctgcg
    20461 cctcgcgaat gagcggcccc gtcgccgccg ggttgagcag cgccggcaac accccgaagg
    20521 cgctgaggat gctgtcgcgg gcggcggcaa gggtctctgc cgtcatgctc cggctcaggt
    20581 ccggcgagat gctggaaggc cgccagtcgg cggccggtgc cgggccgccg gccgcactga
    20641 cgttcacgct ctcgcgcagc accaccttgc cgcgctgtcc cctgaagctc cggccgagcg
    20701 cctgcaagtc ggtctccggc gcctctggaa acgggacaat ctggctgccg atcgggctct
    20761 gcgcgaacac ctcggccagg gcggcctcga cggcgtgcaa cagctcgccc gtcaggctgg
    20821 cccgccggag cggcgctgtc ccgagccatg gcgcagccgg gtccgagccg atgcggatgt
    20881 ggatcacctc ggcggccaga gcggtctgcg tgctgccgcc gccggcttcg ctcaggctga
    20941 tcctgtacgc cgtcggcacg ccgtccctgg tgcgcagatc ccagtcgctg cacggcacca
    21001 ggcgatcatc gcggatgaga aagacggcct cgccccgcag ggccacggat cgcgccagga
    21061 gcgccatgag cgagcgcggc agcatatcgg tgccggtcac gtcggccagg gccagagcgc
    21121 cttcccagag cgatatggcg ccctgcaccg tggcggtgag ctcgccgagc ccgcgccggc
    21181 cgctgatgta gctctcgcgc gccgccatga tctcggccgt gaagccagag ccgctggcac
    21241 ggcgctcgat cttctgccgg cggaatggcc accacctcat cgcagcgccc gccatggccg
    21301 tagaagatca gcggcccccg agagcaccat cgcccgggct gcccatgcgc tcggtcgacc
    21361 cgtgtggctg tagtcgccaa gcgtctcgct cgtgtagccg gccatcgtgg aaggctcggc
    21421 agcgatcgag gcgaagaact cggccagccg tcgcgccgcc tcgagcacca ccgccggcgg
    21481 atcggtatcg tcgccgacag tgccggtgaa gcgaaaggac gtgtccgcat cgagcgcgta
    21541 gcccagcggt gccgccatct cggcgaggct ggccgatgcc caggtgccgc cacctgccca
    21601 gccctcgacg gtggcgatcg agagcggtgc cagcggcggc aggaacgtgc ccgggccctc
    21661 gacgatcatc accaccggac gggcgggcca gcgatggctg atccaggcct cgaggcgctg
    21721 ccaggtggcc gcggcatcgg caacggtgcc ggtgaagccc ggaatggcgg gccagctcgc
    21781 cggttgaccc tcggtgatcg agagcacggt cgcccgggtc atcgccgcca ccgccgcatc
    21841 gccgccagcg ggctcggcac ggccggccgt ccgggcgtcc agcaccgggc ctcgacctga
    21901 gccgcgtcgt atgccggccg ggtcacgatg ctcagctcga agagcaaggc ctgcaagatc
    21961 g
//
LOCUS       DAOTFI010000050        21781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2868_length_21781_cov_6.909003, whole genome shotgun sequence.
ACCESSION   DAOTFI010000050 DAOTFI010000000
VERSION     DAOTFI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21781
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_21781_cov_6.909003"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="P5558_06950"
     CDS             complement(<1..509)
                     /locus_tag="P5558_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24081.1"
                     /translation="MRQRVSPGLLAMLPVMALILVGFALPILLVLLYSLMPPRTFSLA
                     GEPTLANYWAAVTEGYLLPLGWSLLLAGLTTLLCLVLAWPTAKALVRFAGRFATLATI
                     LIALPIFISESVRLFGTALFMMPGGGIFAGSLNALFGLKIGSILYTRTATLLGMVYIH
                     FPFMLFPMVL"
     gene            complement(515..1522)
                     /locus_tag="P5558_06955"
     CDS             complement(515..1522)
                     /locus_tag="P5558_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24082.1"
                     /translation="MTTSGPILSLVGLTRRFGEIAAVDDIDLEILDGEFFTIVGPSGS
                     GKSTLIRILAGLERVDSGAVLLRGRAITDVPANKRPTAMVFQSLALFNHKTVGENIEF
                     APKMRGVDPAARRARALELMDLVRLPEAYYARPVTRCSGGERQRVALARALASDPEIL
                     FFDEPLSAIDYRLRKMLEVELKDLHRETGKTFIYITHSLEEAMVMSDRIGIMRAGRLV
                     QIGPPLEIYQQPRSRFVAEFMGEVNVLRASEFGVAGEGWLVVRPEQLRPLAPGEAADV
                     VFEARIVNDYLLGSRKQFHARADERMLIGEIAVAAASVPGPGEPVRLGFDLGDAVRVA
                     E"
     gene            complement(1532..2734)
                     /locus_tag="P5558_06960"
     CDS             complement(1532..2734)
                     /locus_tag="P5558_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PotD/PotF family extracellular solute-binding
                     protein"
                     /protein_id="HRY24083.1"
                     /translation="MRDIRRLQQLARRNPVRDMGLRSMAMTRRRLMAGIGAAGTLAAA
                     GPRLALAQDRPLALLTWDAYADPRLLELWREKTGETVKTEIHISDPTSVNRLRAGETS
                     VWDFININNPWARTQLWPEGLIRDLPQDRFQPLYEAMMPKFAPPYHWAMSQDGEHLLG
                     VVQRYETFDFVVNSDVISPELAKNEGWDLFNNPDFSQRYGILAYEDWNVMDICMGAGV
                     HPFKPKSDEDIAKFEATAKRWIDNAKLITTDFVQLNLGIINGEIDLYFTGGTYTTTSA
                     RLEGVSNLHAVTPSSGPADGKGGINWIELNSAVANPNEHPHALDFLAWIAEVEPAWII
                     AHGNGNLQPVAQMADPELFAKFTSEELEALQWDEFDERIANAVEFDIVPDYDRLYDIY
                     SAAMRTRQ"
     gene            2965..3714
                     /locus_tag="P5558_06965"
     CDS             2965..3714
                     /locus_tag="P5558_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRY24084.1"
                     /translation="MLDPDEPAPFVLENTGGGSPWLLTCEHGGRRLPRALGDLGLPAA
                     AFERHIAWDIGALGVARSLARALDAELVHQPYSRLVVDCNRPPTRPSFIPEISEATVI
                     PGNLGLAPEARLAREKAIWRPFQDAVAEAVGGRAGLLAIHSFTPVYKEQVRPWQLGFL
                     HARAERLALDLQRWFAAEAQIEAALNEPYCVDADDYTIPVHGDARGRPAVLVEIRQDL
                     IADARGQARWADLLRRAVLNLEPPAQPAQSE"
     gene            3725..5041
                     /locus_tag="P5558_06970"
     CDS             3725..5041
                     /locus_tag="P5558_06970"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRY24085.1"
                     /translation="MHDTHEGIVFFVTSDFSGLVRGRGVPARNLPAFLETGCGWVPAD
                     QAINAFDVIGEGNPFGSSGDLRLMPDPATEVRIAGNAGRPPWHFFLADIQSLDGAPWS
                     CCARGFLKAALADLEQRFGLGLSVAFEHEFTLLGDPAPAPGFSMRAFRRQETLVTAIA
                     SALRAAGAAPEMILPEFGPGQFEITLPPAPALTAADRAVVFREVVRDVAERQGSRATF
                     APKIRPEGVGNGVHIHLSLNDSKGRPVTYDADGPGELSAPAAAFAAGVLRHADALVAL
                     TAPSVVSYLRLKPHHWATAFACLGRQNREATIRICPRARAGGDPAAGHHLEYRAADAT
                     ASPYLALGALVRAGLAGLAENLPAPPLVDTDPAELDAAECERLGVRPLPASLAAALDA
                     LAADKVAMTWAAPDFWACYLAMKRSEWAGLADYDHVALCDAYSRVY"
     gene            5046..5660
                     /locus_tag="P5558_06975"
     CDS             5046..5660
                     /locus_tag="P5558_06975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRY24086.1"
                     /translation="MDNDRLPRDRRLGPERTAVLLVDVQLSEIVPEHEQEKPDYWRMI
                     HERALPAQQRLLAAARERRCEVLYTVIEALTADGRDRSLDHKLSGILVPKGSPLARVI
                     PAVAPLADEIVLPKTSSGVFNSTAIDYVLRNLGIDNLIVCGFLTDQCVDMAVRDAADK
                     GYYVVCAEDACATHTAERHARALEAFGGYCRVQTVDEVIDTFSH"
     gene            5767..7323
                     /locus_tag="P5558_06980"
     CDS             5767..7323
                     /locus_tag="P5558_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRY24087.1"
                     /translation="MADTTGAITSTHGRKTFLDRFLDGVERVGNKVPHPAVIFLVLIA
                     IVILLSHLLYLLDISVTYPVVDYLTDEVVERTTAVRSLLTADGLRFMLTSPVPNFMSF
                     GPVAVILVAMIGVGLAEESGLIKALVRQIVVVAPRSAITFIIVLLGVVSSIAADAGYL
                     VLVPLGAAAFHSLGRHPLAGLAAAFAGVAGGFLTNLFVTPTDAILTEITNDAIRLLNP
                     TLSTGLTGNLFFMIASTLLVAIVCTVLTERFIEPHLGPYQGGVPIDTGEGVTDAERRG
                     LRFTLWTLAGFVVVIGLLSAPPGAVLRHQETGAILGGSPFMDGLIVLIMLLFLALGIA
                     YGKGAGTINTVTEGVNAVVKTFNSLAGLVFLLLVIAQFIAFFNFTNIATVTAVGLADL
                     LGDVQIGTVGLLVGFVVVIGIVDLLITGAIAKWAIFAPVFVPLFMQLGVEPDFVLAAY
                     RVGDSPANVITPLNVYLAMIVAFAQKYQPESGVGTVVALMLPYTVVLFIVWTLMLVGW
                     YLLGIPLGFG"
     gene            7361..8743
                     /locus_tag="P5558_06985"
     CDS             7361..8743
                     /locus_tag="P5558_06985"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRY24088.1"
                     /translation="MSELDAVLARIDEDFDQSLERLFTLLRIKSISTDSAYAGDCRAC
                     ADWLVEDLRSIGFEASARTTPGHPIVVAHDRNAGGTSALFYGHYDVQPVDPIELWEHD
                     PFEPFIQTRTDGSRAIRARGASDDKGQVMTFVEACRAWKHVTGRLPIALSVLIEGEEE
                     SGGVNLPPFLETHKDELLADIGLICDTSMWDRETPAINTMLRGLCGEEIVITAADRDL
                     HSGFYGSAAANPNHVLARIIADLRDASGGVTIPGFYEGVPELPEALRRDWESLGFDAA
                     AFLGAIGLSIRAGEKGRSVLEMVWSRPTCEVNGMGGGYQGEGFKTVIPSRASAKISFR
                     LVFDQDPHKIRDAFRAFVRERVPADCKVEFKEHGSGRAIRFPIDAPAFAQTRDALGEE
                     WGRPAVFIGGGGSIPVTAELKGKLGMDVVLAGFALDDDRIHSPNEKYELSSFKKGMRS
                     WARILHALAG"
     gene            8784..10616
                     /locus_tag="P5558_06990"
     CDS             8784..10616
                     /locus_tag="P5558_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRY24089.1"
                     /translation="MTIDLALVLALLAAAILMFVLNRPRMDVVALIMIAALPLTGVLT
                     VGEAIAGFADSNIVLIAVLFVLGESLVRTGVARRLGDWINARAGGSETRLLVLLMVSV
                     AGLGSMMSSTAVVAIFIPVVMRICKNTGTAPGKLMMPMSAAALVSGMLTLVATAPNLV
                     VNAELVRQGFEGFNFFSVTVFGLPVLALAVGYMLLTRGWLPATIRESTSQRRRPTFRE
                     FAEKYQLGGREHRVRVRPDSPLAGKRLDEVELRATGINLIALERPGRRRLDIVRPDAR
                     TELRAGDILLLDVRSSDVETAKLKDQYGVDTLILSGAGVYFSDRAQDLGMVEAIIPAE
                     SRLLGLTVLQARMRTGSGLTVIGLRRGQAVIAEHLLEEKLEVGDTLLLTGFWKDIRRF
                     QQQSHDVVMLDMPAELDEILPAADKAPQALAVLALVVALMVFEVVPNVHAVLLGCLLL
                     GLLRCVDMASAYRSINWPSLVLIVGMMPFSLALQRTGGVDLAANGLLALVGEGSPRIV
                     LGAIFLITAVLGMFISNTATAILMAPVALAVAIDLGASPYPFAMTVALAASTAFMTPV
                     SSPVNTLVVGPGNYVFADFVKIGVPLSILVMIACVLLVPIVLPL"
     gene            complement(10613..11047)
                     /locus_tag="P5558_06995"
     CDS             complement(10613..11047)
                     /locus_tag="P5558_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HRY24090.1"
                     /translation="MTDKVDVKYRTNATATGGREGRSKTADGKVDVQLSTPKELGGGG
                     GAGTNPEQLFAAGYSACFLGALRFVAGNEKVTIPDDATVAAEVGIGPREDGTGFGLEV
                     ALTLKVPGMDRAKAEELMKKADVVCPYSHATKGNIKKTLSVG"
     gene            complement(11073..11819)
                     /locus_tag="P5558_07000"
     CDS             complement(11073..11819)
                     /locus_tag="P5558_07000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24091.1"
                     /translation="MSGPLAGRVVLLTGAAGGFGQAIAEAFRKEGAELGLVVRRRGQL
                     PPEAGLVIVADLADPAAPGGIVAAVIERFGRLDVLVNNAGVAPRLGLDNATPEAWRAA
                     FAVNLDAAMFLTQAAAPHLARTGGSIVNIASEMGRRASPASIAYSVSKAALIQLTRCA
                     ALALAGDGVRVNAIAPGPVDTPMLRHSIAQRGIDLEEGLAGYRSRVPSRRLGSVDEVA
                     RAVLYVASPDAAYMTGAVLAIDGGTTAGEA"
     gene            complement(11816..12307)
                     /locus_tag="P5558_07005"
     CDS             complement(11816..12307)
                     /locus_tag="P5558_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY24092.1"
                     /translation="MQVEAVLQEILDRQAIAGLIHAYCRHFDRNEPKALARLFTADAR
                     VDYGPEFPDLVGRPAIEQGVARGLETLFAATSHHVSNIEIAFDGPDAARSDCYLYAWQ
                     RYCDGRPDGELWASYHHRLVRTAEGWRIALLVLSAAGARSFHRATMHGIGRRPAGEPE
                     GEA"
     gene            12389..13147
                     /locus_tag="P5558_07010"
     CDS             12389..13147
                     /locus_tag="P5558_07010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24093.1"
                     /translation="MGMLDGKVVLLTGASKGIGAATAGVLLREGAALVAQYGGDRAGA
                     EAATAGGDALLAQADFRDPLAVDRLWREALAWRGRVDVLVNNAALMLTHAGFDAGQAE
                     WDAVWAETLAVNVTAPARLTRHAVNHFREAGGGVLVTVSSWAAQRGSGNPDLVAYSAS
                     KAAVKAMTQTIARGFARESILAYIVAPGVVRTRMSEDFAAMQGGEDKVTATLAMGEWV
                     PPADVAECTAFLASGRARHLSGATLDVNGASYIR"
     gene            13225..13353
                     /locus_tag="P5558_07015"
     CDS             13225..13353
                     /locus_tag="P5558_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24094.1"
                     /translation="MPEIMDKTEARQAETTGHVRYILVISTIAAIVVLGALVLYFA"
     gene            complement(13360..14481)
                     /locus_tag="P5558_07020"
     CDS             complement(13360..14481)
                     /locus_tag="P5558_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HRY24095.1"
                     /translation="MTVDRTPHLFRPFTQRGLTFKNRVCVSPMCQYQAIDGRMTDWHY
                     DHHVRFAMGGVAVGMVEATGVTPEGRITHGCTGLWEDGQIAPMARVTAAYKRYGCVPA
                     IQIGHAGRKASTARPWEGAHPLGADEDPWQTIAPSAIPVREGWHTPKAMDEADLARIR
                     HAFRDAAKRALAAGFEVVELHGAHGYLLHSFLSPLTNRRNDAYGGDLEGRMKLPLEVA
                     RIVRESWPENLPVWYRVSAQDGVEGGLELADTIELVKRLKAHGIDLVDCSSGGIAGSP
                     SLATVHPGPGFQVPFADAIRKGADIATMAVGFITEAEQAEAILAEGKADMIAIAREHL
                     ADPVWAYRAALALGAPEAHGLLPHLYHWYLERRARLQRS"
     gene            complement(14478..15413)
                     /locus_tag="P5558_07025"
     CDS             complement(14478..15413)
                     /locus_tag="P5558_07025"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY24096.1"
                     /translation="MHGHEFRGSYTVQVTPFTADGSDIDEAAQRRFVDWQIAEGVPGL
                     IILGTSGEFLAVSDAERTRLIEVTVDQAAGRIPVLVGTMNAHTPTAVRHSKEAERLGA
                     KGLMIVPPYYYTPTEDEIFNYYKAISEAVTIPIMLYNNPFTSNVDMKPALVAKLTRAF
                     DNIRYIKEASMDVGRVFDIVEATEGVMNVFAGERIVESYLLGAVGYVNPFGNYIPGPS
                     SRICDLLAEGKVEEAKAIQRHISVIEHTIAEGHPTWGHQCYSKALAALVGYPMGDVRP
                     PLTSFASLGQEGRDRLAKLRRVMQDLGVLPAEAAE"
     gene            complement(15420..16271)
                     /locus_tag="P5558_07030"
     CDS             complement(15420..16271)
                     /locus_tag="P5558_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY24097.1"
                     /translation="MADLKLSASTFSYIYTENGLEASLHLADMGFEAIEMIVFAPHCW
                     PPLLTATERKAYQEQLAARGLSLSSFCYPLLDNNPNAPDALMRRYTLDRYREHIDLAA
                     EWGCPYVVAIPGPVNSLIDPPYAWMLNWFVEGVKELVAHAKGTGVEILIENVPFTFLP
                     TVDDMLKVLDLVGEPSVGVNFDVCNSAFIKEDPAAAIRKLGPRCRNVHISDSGFDVFK
                     HHGLGKPGGIVDPAPVAAALRDIGYEGYTVLEIIANKILPDSTPDEDFLASHAILRQH
                     GWADLRK"
     gene            16384..17556
                     /locus_tag="P5558_07035"
     CDS             16384..17556
                     /locus_tag="P5558_07035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY24098.1"
                     /translation="MPPLPLKALPDRIDTAVIGGGIAGCAVAFELARRGVEVAVLEAG
                     DLAAMASGANSGSLHAQIPMEPFLLEGEAWVRGFASTTRLLAASIELWRGLEALLETP
                     LEVSLKGGLLVAGSEAQLAAMARKVALERSLGLDSHLLGQSELGEVAPYLAPGLAGAA
                     FCAQEGKASPLLAGPAFAAAARRKGAQVHPFTPVRTISEAGSGYRLETTRGVLLARRV
                     VIATGADAGRTASLLGLDLPLWGEPIQASITEPVAAIIPHLVYFAGDKLTLKQTASGG
                     LLIGGGWDARLDGRGRPTVDGGNLAQNLAVAARVVPAVARARLLRSWPGLVNANADWR
                     PVLGPVPGRPNLHFLCFPWMGFTAAPICARLVAQAIVGEPPDLPIEEFSIARYGAG"
     gene            complement(17668..18411)
                     /locus_tag="P5558_07040"
     CDS             complement(17668..18411)
                     /locus_tag="P5558_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRY24099.1"
                     /translation="MPHLAVADLTATYPGTDTPVLRDVSLEVQKGEIVSLIGPSGSGK
                     STLLRVLVGLLPPTSGAVRIDGEAIDYTSRRSLKLARDRLAIVFQQYNLFQNMTAMGN
                     VTIAPIRIKGWKRPEVEADAARLLAKVGLAEKHESYPDQLSGGQQQRVAIARALALKP
                     EILLLDEVTSALDPELVGEVLDTIRVLAADGMTMLIVSHEMGFVREVSSKVVMMDQGS
                     IVETGPPAQVFDAPRTDRCREFVSRILRH"
     gene            complement(18389..19078)
                     /locus_tag="P5558_07045"
     CDS             complement(18389..19078)
                     /locus_tag="P5558_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY24100.1"
                     /translation="MLDRIISEAPRFFTYYNMVFLLEAMGRTLLMALIGVSLGFVLGF
                     LLDFARRTPGRAWLPLRVAATLYVETFRRIPFLVILFIVLFAIQGFRFPVSLFGVAMI
                     AICVISSAFCAEIIRAGFDSIHQTQWDTAAVMNFGRLQTLRHVVLPQAWRVILPPAFA
                     FMVMFIKDTALASQLGVVELTFAGKVLSTRGFSAMLVFGTVLVLYFALSYPLSRLGGW
                     LERRLAPSRGR"
     gene            complement(19078..19752)
                     /locus_tag="P5558_07050"
     CDS             complement(19078..19752)
                     /locus_tag="P5558_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY24101.1"
                     /translation="MTYTFQWGEALKHLPYLLEGAWLSLALAFLGFWGGALIGLFGAV
                     GKVYGGRLVYRIVDIYVVFFTNTPALVQIFFLFYALPEVGILLAPFTAVLIGLVLNSG
                     AYLTEIQRAGFVSVRQTEIEAAEVLGMSLAQIVRYVIVPHIAKTLFAPLSSFYIWLVL
                     GTSMASIFGVEELTGRAINISTTNFRTIEVFSVTALIYIAITILASAVLALIGRYAFR
                     VRAKIF"
     gene            complement(19809..20618)
                     /locus_tag="P5558_07055"
     CDS             complement(19809..20618)
                     /locus_tag="P5558_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY24102.1"
                     /translation="MLAHVRTCFVAITALAMLPLAAADARSLDEIISSGTIKIGVNPN
                     FPPMSAYGATNEFEGFDIDVGNRIAEALGVEAEFVPTETPQRVPFITAGTIDISLGAL
                     TRTAERAKLIDYTVPLHTEAMAALTTDKVTAESWKDLNSKDITLVNIRGTWPVDFNKE
                     NLPETEMLLVDSIADTVRSLAQGRADALVENIDFFMAQTENYPDVNWRVLPDTIFVGY
                     CGIGVEKGNAGLVNFLNILLYDLHSSDFINETWRKWYGADMLVKVEPNPFF"
     gene            complement(20765..>21781)
                     /locus_tag="P5558_07060"
     CDS             complement(20765..>21781)
                     /locus_tag="P5558_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY24103.1"
                     /translation="GAMTTGACQTLLRAYRVAPGSRVLVAGNGPLNLQVAAELLRAGV
                     DVAALVETAPSPWRRPAAMLAMARTAPDLVRNGFSDLWLLRRRGVPLLHGHQLVRIEG
                     EGSVEAAAVARIDEAGRPVAGSERRFAVDTVASGYGFLPQGELSRALGCDHEHDPGFD
                     QLRVARDADCRSSVPEVFVADDAGGLGGARVAQAQGAIAGARAAADLGFGPDAAVIGA
                     ARAALARGRRFQKALWRLYAAPLPGHALATPETLVCRCEEVGLATLQAAIAGGAASIG
                     ALKRRTRAGMGRCQGRYCAPVMAAMIAEATGRAVDEFALMAPRAPIRPVPIAAIAGID
                     ESAG"
BASE COUNT         3260 a         7852 c         7281 g         3388 t
ORIGIN      
        1 agcaccatgg gaaagagcat gaaggggaag tggatataga ccatgccgag gagggtggcg
       61 gtccgcgtgt agaggatgct gccgatcttc agcccgaaga gcgcgttcag gctgccggcg
      121 aagatgccgc cgcccggcat catgaagagg gccgtgccga agaggcggac actctcggag
      181 atgaagatcg gcagggcgat caggatggtc gcgagcgtgg cgaagcggcc ggcgaatcgc
      241 accagcgcct tggcggtcgg ccaggcgagg acgaggcaaa ggagcgtggt gagcccggcg
      301 agcaagagcg accagccgag cggcaaaaga tagccctcgg tcacggcagc ccagtagttg
      361 gcgagcgtcg gctcgccggc gagcgagaag gtccggggcg gcatcaggct gtagaggagg
      421 acgagcagga tgggcagggc gaagcccacg aggatgagcg ccatgaccgg cagcatggcg
      481 agaaggcccg ggctgacccg ctgccgcacc ggggctactc cgccacccgc acggcgtcgc
      541 cgagatcgaa gccgagccgc acgggctcgc ccggccccgg cacgctcgcc gccgcgacgg
      601 cgatctcgcc gatcagcatg cgctcgtcgg cccgggcgtg gaactgcttc ctcgatccca
      661 gcaggtagtc gttgacgatc cgggcctcga agaccacgtc ggccgcctca cccggcgcca
      721 gcggccggag ctgctcgggc cgcaccacga gccagccctc gccagcgacc ccgaactcgc
      781 tagccctcag cacgttcacc tcgcccatga actcggcgac gaagcgcgag cgcggctgct
      841 ggtagatctc gagcggcggc ccgatctgga ccagccggcc ggcccgcatg atgccgattc
      901 ggtccgacat caccatcgcc tcctcgaggc tgtgggtgat gtagatgaag gtcttgccgg
      961 tctcgcgatg caggtccttg agctcgacct cgagcatctt ccgcagccgg tagtcgatcg
     1021 ccgagagcgg ctcgtcgaag aagaggatct cggggtcgga cgccagtgcg cgggccagcg
     1081 ccacacgctg ccgctcgcca ccggagcagc gcgtcaccgg ccgcgcgtag taggcctcgg
     1141 gcagccgcac caggtccatc agctcgagcg cccgggcccg acgtgccgcc ggatcgacgc
     1201 cccgcatctt cggcgcgaac tcgatgttct cgccaaccgt cttgtgattg aagagcgcca
     1261 gcgactggaa gaccatcgcg gtcggccgct tgttggccgg cacgtcggtg atcgcccggc
     1321 cgcgcagcag caccgcacca ctgtcgaccc gctcgagccc ggcgaggatg cggatcaggg
     1381 tcgacttgcc gctgccggac gggccgacga tggtgaagaa ctcgccgtcc aggatctcga
     1441 ggtcgatgtc gtcgaccgcg gcgatctcgc cgaaccggcg cgtcaggccg accaacgaga
     1501 ggatcggccc gctcgtcgtc acagccgcgg cctactggcg ggtccgcatg gcagccgagt
     1561 agatgtcgta gagccggtcg tagtcgggca cgatgtcgaa ctcgacggca ttggcgatgc
     1621 gctcgtcgaa ctcgtcccac tgcagcgcct cgagctcctc gctggtgaac ttggcgaaga
     1681 gctcgggatc ggccatctgc gccaccggct gcaggttgcc gttgccgtgg gcgatgatcc
     1741 aggcgggctc gacctccgcg atccacgcga ggaagtcgag ggcgtgcggg tgctcgttcg
     1801 ggttggccac cgccgagttg agctcgatcc agttgatgcc gcccttgccg tcggccgggc
     1861 cgctcgaggg cgtgacggcg tgcaggttcg agactccctc gagcctggcg ctcgtcgtcg
     1921 tgtaggtgcc gccggtgaag tagaggtcga tctcgccgtt gatgatgccg agattgagct
     1981 ggacgaaatc ggtggtgatc agcttcgcgt tgtcgatcca gcgcttcgcc gtcgcctcga
     2041 acttcgcgat gtcctcgtcc gacttcggct tgaagggatg aacacccgcc cccatgcaga
     2101 tgtccatcac gttccagtcc tcgtaggcga ggatgccgta gcgctggctg aaatcggggt
     2161 tgttgaagag gtcccagccc tcgttcttgg cgagctcggg cgagatgaca tccgagttca
     2221 cgacgaagtc gaaggtctcg tagcgctgca cgacgcccag cagatgctcg ccatcctggc
     2281 tcatcgccca gtgatagggc ggggcgaact tcggcatcat ggcttcgtag agcggctgga
     2341 agcggtcctg cggcaggtcg cggatcagcc cctcgggcca gagctgggtg cgcgcccatg
     2401 ggttgttgat gttgatgaag tcccagaccg aggtctcgcc ggcgcgcagc cggttgaccg
     2461 aggtcgggtc ggagatgtgg atctcggtct tgacggtctc gcccgtcttc tcgcgccaga
     2521 gctcgagcag ccgcgggtcg gcataggcat cccaggtgag cagcgccaga ggccggtcct
     2581 gcgccagggc cagcctcggc cccgccgcag ccagcgtgcc ggcggcgccg atgcccgcca
     2641 tgaggcggcg gcgggtcatc gccatggaac gcagccccat gtcgcgaacc gggttcctcc
     2701 gggctagctg ctgcaggcgg cggatgtcgc gcatcgttcc ctctccctgg ttgcgccgcc
     2761 gggcgcccgc ggcgaagagc agcgctcggg ccccggttgt gcggcatgcg gtcggttgtg
     2821 cggatagtgc aagtgccggg aagcctgtca aggcgcctcc cccctcttgt catgggcatc
     2881 ccgtctcccg ccgacctctg atagacaata aggtgtggac cggcccaccg tgcttccctc
     2941 ctcccacaac cgaggcgccg ccccttgctc gatcccgacg aaccggcccc cttcgtgctc
     3001 gagaacaccg gcggcggcag cccgtggctg ctcacctgcg agcacggcgg ccggcggctg
     3061 ccccgtgcgc tgggcgatct cggcctgccg gccgccgcct tcgagcgcca catcgcctgg
     3121 gacatcggcg ccctcggcgt cgcgcgaagc ctcgcccggg cgctcgacgc cgagctggtg
     3181 caccagccct attcgcgcct cgtcgtcgac tgcaaccgcc cgccgacccg ccccagcttc
     3241 atccccgaga tcagcgaggc cacggttatt ccgggcaatc tgggcctcgc ccccgaggcc
     3301 cgcctcgccc gcgagaaggc gatctggcgg cctttccagg atgcggtggc cgaagccgtc
     3361 ggcggccggg cgggcctgct cgccatccac agcttcacgc cggtctacaa ggagcaggtg
     3421 cgtccctggc agctcggctt cctccacgcc cgggccgagc gtctggcgct cgacctccag
     3481 cgctggttcg ccgccgaggc gcaaatcgag gcggccctga acgagcccta ttgcgtcgac
     3541 gccgatgact atacgatccc ggtgcacggc gacgcgcgcg gccggcccgc cgtgctcgtc
     3601 gagatccgcc aggacctgat cgccgatgcc cgtggccagg cccgctgggc cgacctcctg
     3661 cgccgcgcgg tcctgaacct cgagccacca gcccaaccag cccagagcga gtaggcgcgc
     3721 caccatgcac gacacccacg agggcatcgt cttcttcgtc accagcgact tctccgggct
     3781 ggtccgcggg cgcggggtgc cggcccggaa cctgccggcc ttcctcgaga ccggctgcgg
     3841 ctgggtcccg gccgatcagg cgatcaatgc cttcgacgtc atcggcgagg gcaacccgtt
     3901 cggctcctcg ggcgacctcc ggctgatgcc cgacccggcg accgaggtgc ggatcgccgg
     3961 gaatgccggg cgacccccct ggcacttctt cctcgccgac atccagagcc tcgacggcgc
     4021 cccctggtcc tgctgcgcgc gtggcttcct caaggccgcg ctcgccgatc tcgagcagcg
     4081 cttcgggctc ggcctcagcg tcgccttcga gcacgagttc accctgctcg gcgatcccgc
     4141 cccggcgccg ggcttctcga tgcgcgcctt ccgccggcag gagacgctgg tgacggccat
     4201 cgcctcggcc ctgcgcgccg ccggtgccgc acccgagatg atcctgccgg agttcggccc
     4261 cggccagttc gagatcacgc tgcccccggc cccggcgctc accgccgccg accgcgctgt
     4321 cgtgttccgc gaggtggtcc gcgacgtcgc cgagcggcag ggcagccgtg ccaccttcgc
     4381 ccccaagatc cggcccgagg gcgtgggcaa cggtgtccac atccatctca gcctcaacga
     4441 cagcaagggc cgaccggtca cctacgacgc cgatggcccg ggcgagctca gcgcgccggc
     4501 cgccgccttc gccgccggcg tgctccgcca tgccgatgcg ctggtcgccc tgacagcacc
     4561 ctccgtcgtc tcctatctgc gcctgaagcc gcatcactgg gccacggcct tcgcctgcct
     4621 cggccggcag aaccgcgagg ccaccatccg catctgcccc agggcgcgcg ccggcggcga
     4681 cccggccgcg ggccatcacc tcgagtaccg cgccgcggac gccacggcct cgccctatct
     4741 ggcgctcggt gccctcgtcc gggccggcct cgccgggctg gcggagaacc tgccggcgcc
     4801 gccactggtc gacaccgacc cggccgaact cgacgcggcc gaatgcgagc ggctgggcgt
     4861 gcggccgctg ccggccagcc tggcggccgc cctcgatgcg ctcgcggccg acaaggtggc
     4921 catgacctgg gccgcccccg atttctgggc ctgctacctc gccatgaagc ggagcgagtg
     4981 ggccggcctc gcggactacg accatgtcgc cctctgcgac gcctacagcc gggtctactg
     5041 acgccatgga caacgaccgc ctgccgcgcg accgacggct cggacccgaa cgaaccgccg
     5101 tcctcctggt cgacgtccag ctctccgaga tcgtgccgga gcacgagcag gagaagcccg
     5161 actactggcg catgatccac gagcgcgccc tccccgccca gcaacgcctg ctggccgcgg
     5221 cgcgcgagcg ccgctgcgag gtcctctaca ccgtcatcga ggcgctgacg gcggacggcc
     5281 gcgatcgctc gctcgaccac aagctctcgg gcatcctggt gcccaaaggc tcgccgctcg
     5341 cccgggtcat cccggcggtg gcaccactcg ccgacgagat cgtcctgccc aagacgtcgt
     5401 ccggcgtctt caactcgacc gccatcgact acgtgctgcg caatctcggc atcgacaacc
     5461 ttatcgtctg tggcttcctt accgaccagt gtgtcgacat ggcggtgcgc gatgccgccg
     5521 acaagggcta ctacgtggtc tgcgccgagg acgcctgcgc cacgcacacg gccgagcgcc
     5581 acgcccgggc gctcgaggcc ttcggcggct actgccgggt gcagacggtc gacgaggtga
     5641 tcgacacgtt ttcgcactag gcaaggccca ccggaagagc tagcccattc gccgtcgaca
     5701 agcagaagcc atgttcaggg cgccgcccga gcgcgaccgc ggcgaatgat ccgggagtca
     5761 tatggagtgg ccgacacaac gggcgccatc acgtccacgc acgggcgcaa gaccttcctc
     5821 gaccgcttcc tcgacggggt cgagcgggtc ggcaacaagg tgccgcatcc cgccgtcatc
     5881 ttcctcgtcc tgatcgccat cgtcatcctt ctctcgcacc tgctctatct cctcgacatc
     5941 agcgtcacct acccggtcgt cgattacctg accgacgagg tggtcgagcg gacgacggcc
     6001 gtgcgcagcc tgctcaccgc cgacgggctc cgcttcatgc tgacctcgcc agtgcccaac
     6061 ttcatgagct tcggcccggt ggcggtgatc ctggtcgcca tgatcggcgt cggcctcgcc
     6121 gaggaatccg gcctcatcaa ggcgctggtc cgccagatcg tcgtcgtcgc gccgcgctcg
     6181 gccatcacct tcatcatcgt gctcttgggc gtcgtctcga gcatcgccgc cgatgccggc
     6241 tatctggtgc tcgtgccgtt gggtgccgcc gccttccaca gcctcggccg gcacccgctc
     6301 gccggcctcg ccgcggcctt cgccggcgtc gccggcggct tcctcaccaa cctcttcgtc
     6361 acgccgaccg acgccatcct gaccgagatc accaacgacg ccatccggct gctcaacccg
     6421 accttgtcca ccggcctgac gggcaacctc ttcttcatga tcgcctccac ccttctcgtc
     6481 gcgatcgtct gcacggtgct caccgagcgc ttcatcgagc cgcatctcgg cccctaccag
     6541 ggcggcgtgc cgatcgacac cggcgagggc gtcaccgacg ccgagcgccg gggcctgcgc
     6601 ttcacgctct ggacgctggc cggcttcgtc gtcgtcatcg gcctcctgtc ggccccgccc
     6661 ggcgccgtgc tgcgccacca ggagaccggt gcgatcctcg gcggctcgcc cttcatggat
     6721 gggctgatcg tcctgatcat gctgctcttc ctcgccctcg gcatcgccta cggcaagggt
     6781 gcgggcacga tcaacacggt gaccgagggc gtcaacgcgg tggtgaagac cttcaacagc
     6841 ctggccgggc tggtcttcct cctgctggtc atcgcccagt tcatcgcctt cttcaacttc
     6901 accaacatcg ccacggtcac cgccgtcggc ctcgccgacc tgctcgggga cgtgcagatc
     6961 ggcacggtcg gcctgctcgt cggcttcgtc gtggtgatcg gcatcgtcga tctcctgatc
     7021 accggggcga tcgccaaatg ggcgatcttc gcgccggtct tcgtgccgct tttcatgcag
     7081 ctcggcgtcg agccggactt cgtgctcgcc gcctaccgcg tcggcgattc cccggccaac
     7141 gtcatcacgc cgctcaacgt ctatctcgcg atgatcgtcg ccttcgccca gaaatatcag
     7201 ccggagtccg gcgtcggcac ggtcgtggcg ctgatgctgc cctacaccgt cgtcctcttc
     7261 atcgtctgga cgctgatgct cgtcggctgg tacctgctcg gcatacccct gggcttcggc
     7321 tgacgccggc ccgaccacga cactcaacgg gaggtcaccc atgtccgagc tcgacgccgt
     7381 cctcgcgcgg atcgacgagg atttcgatca gtcgctcgag cgcctcttca ccctcctgcg
     7441 catcaagtcg atctcgaccg attcggccta tgccggcgac tgccgggcct gcgccgactg
     7501 gctggtcgag gatctccgct ccatcggctt cgaggcgagt gccagaacca cgcccggcca
     7561 ccccatcgtc gtcgcccatg accggaacgc cggcggcaca tccgccctct tctacggcca
     7621 ttacgacgtc cagcccgtcg acccgatcga gctctgggag cacgatccct tcgagccctt
     7681 catccagacc cggaccgatg gcagccgcgc catccgcgcc cgcggcgcct cggacgacaa
     7741 gggccaggtg atgaccttcg tcgaggcctg ccgggcctgg aagcacgtca ccggccggct
     7801 gccgatcgct ctttcggtgc tgatcgaggg cgaggaggaa tccggcgggg tcaaccttcc
     7861 ccccttcctc gagacgcaca aggacgagct tctggccgat atcgggctga tctgcgacac
     7921 cagcatgtgg gaccgcgaga cgccggccat caacaccatg ctcaggggcc tctgcggcga
     7981 ggagatcgtc atcaccgccg ccgaccgcga cctgcattcc ggcttctacg gctcggcggc
     8041 ggccaacccc aaccacgtgc tcgcccggat catcgccgat ctccgcgacg cctcgggcgg
     8101 cgtcaccatc ccgggcttct acgagggcgt gccggagctg ccggaagcgc tccgccgcga
     8161 ctgggagagc ctgggcttcg atgccgccgc cttcctcggc gccatcggcc tgtcgatccg
     8221 ggcgggcgaa aagggccgct cggtgctcga gatggtctgg tcgcggccga cctgcgaggt
     8281 caacggcatg ggcggcggct accagggcga aggcttcaag acggtgatcc cgtccagggc
     8341 ctcggccaag atctcgttcc gcctcgtctt cgaccaggac ccgcacaaga tccgggacgc
     8401 ctttcgcgcc ttcgtgcgtg agcgcgtgcc ggccgactgc aaggtcgagt tcaaggagca
     8461 cggctcgggc cgcgccatcc gtttcccgat cgacgcgccg gccttcgccc agacgcgcga
     8521 cgcgctcggc gaggaatggg gcaggcctgc cgtcttcatc ggcggtggcg gctcgatccc
     8581 ggtcacggcc gagctcaagg ggaagctcgg catggacgtg gtgctcgccg gcttcgccct
     8641 cgacgacgac cggatccaca gcccgaacga gaagtacgag ctctcgagct tcaagaaggg
     8701 catgcgctcc tgggcgcgca tcctgcacgc cctcgccggc tgagcgcggc acccccttct
     8761 accaatcgac ctggctggag ccgatgacta tcgacctggc gctcgtcctc gcattgctcg
     8821 ccgccgccat cctcatgttc gtgctcaacc ggccgcgcat ggacgtggtg gcgctgatca
     8881 tgatcgcggc cctgcccctg accggggtcc tgaccgtcgg cgaggccatc gccggcttcg
     8941 ccgacagcaa catcgtgctc atcgccgtgc tcttcgtgct gggcgaaagc ctggtgcgga
     9001 ccggcgtcgc ccgccggctc ggcgactgga tcaatgccag ggccggcggc agcgagaccc
     9061 gccttctggt gctcctgatg gtgtccgtcg ccgggctcgg ctcgatgatg agctcgaccg
     9121 cggtggtcgc catcttcatt cccgtggtca tgcgcatctg caagaacacg ggcacggcgc
     9181 ccggcaagct gatgatgccg atgagcgccg ccgccctggt cagcggcatg ctgaccctgg
     9241 tcgccacggc cccgaacctc gtcgtcaacg ccgagctggt ccggcagggc ttcgagggct
     9301 tcaacttctt cagcgtcacg gtcttcggcc tgccggtgct ggcccttgcc gtcggctaca
     9361 tgctcctgac ccggggctgg ctgccggcca cgatcaggga atcgacgagc cagcgtcgcc
     9421 ggccgacctt tcgcgagttc gccgagaagt accagctcgg cggccgcgag caccgcgtcc
     9481 gcgtcagacc cgattcgccg ctcgccggca agcgcctcga cgaggtcgag ctgcgggcga
     9541 ccggcatcaa cctcatcgcc ctcgagcgac cgggccggcg gcggctcgac atcgtccgtc
     9601 ccgacgcccg caccgagctg cgcgccggcg acatcctcct cctcgacgtc cgctcctccg
     9661 acgtcgagac ggcgaagctg aaggaccagt acggcgtcga cacgctgatc ctgagcggtg
     9721 ccggcgtcta cttcagcgac cgtgcccagg atctcggcat ggtcgaggcg atcatccccg
     9781 ccgaatcgcg actgctcggg ctcaccgtgc tgcaggcccg gatgcgcacc gggtccggcc
     9841 tcaccgtgat cggcctgcgt cgcggccagg cggtgatcgc cgagcacctg ctcgaggaga
     9901 agctggaggt cggcgacacc ctcctcctca ccggcttctg gaaggacatc cgccgcttcc
     9961 agcagcagag ccacgacgtc gtcatgctcg acatgccggc cgagctcgac gagatcctgc
    10021 cggccgcgga caaggcgccg caggcgctcg ccgtcctggc gctggtcgtg gccctcatgg
    10081 tcttcgaggt cgtgcccaac gtccacgccg tgctgctcgg ctgcctgctg ctcggcctct
    10141 tgcgctgtgt cgacatggcc agcgcctacc gctcgatcaa ctggccgagc ctggtgctca
    10201 tcgtcggcat gatgccgttc tccctggccc tgcagcgcac cggcggcgtc gacctcgccg
    10261 ccaacggcct cctggcgctg gtcggcgagg gctcgccccg catcgtgctc ggggcgatct
    10321 tcctgatcac ggccgtgctc ggcatgttca tctcgaacac cgccacggcc atcctcatgg
    10381 cgccggtggc gctcgccgtc gccatcgatc tcggcgcctc gccctacccc ttcgccatga
    10441 ccgtggcgct ggcggcgtcg acggccttca tgaccccggt ctcctcgccg gtgaacacgc
    10501 tggtcgtcgg ccccggcaac tacgtcttcg ccgatttcgt gaagatcggc gtgccgctgt
    10561 cgatcctcgt gatgatcgcc tgcgtgctgc tcgtgccgat cgtcctgccg ctctagccca
    10621 ccgacaacgt cttcttgata ttgcctttgg tcgcatggct gtaggggcag accacgtccg
    10681 ccttcttcat gagctcctcg gccttggccc ggtccatgcc gggcaccttc agggtcagcg
    10741 ccacctcgag gccgaagccg gtgccgtcct cgcgcgggcc gatgccgacc tcggccgcga
    10801 ccgtggcatc gtccgggatc gtcaccttct cgttgccggc gacgaagcgg agcgccccga
    10861 ggaagcaggc cgagtagccg gcggcgaaga gctgctcggg attggtgccc gcgccaccac
    10921 cgccgcccag ctccttcggc gtgctgagct gcacgtccac cttgccgtcg gcggtcttcg
    10981 aacgaccctc gcggccgccc gtggcggtgg cattggtccg gtacttcaca tcgaccttgt
    11041 ccgtcatggc gtctctccct cgtgctgtcc cgtcaggcct cgccggccgt ggtgccgcca
    11101 tcgatggcga gcaccgcccc agtcatatag gccgcatccg gcgaagcgac atagagaacg
    11161 gctcgcgcca cctcgtcgac cgagcccagc cgccggctcg gcacgcgcga cctgtagccc
    11221 gccagcccct cctcgaggtc gatcccgcgc tgggcgatcg aatggcgcag catcggcgtg
    11281 tccaccggac ccggcgcgat cgcgttgacc cgaaccccgt ccccggccag cgccagcgcg
    11341 gcgcagcggg tgagctggat caacgccgcc ttggagacgc tgtaggcgat cgatgccggg
    11401 ctcgcgcgcc ggcccatctc cgaggcgatg ttgacgatcg agccccccgt ccgcgccagg
    11461 tgcggcgccg ccgcctgggt caggaacatc gcggcgtcga ggttgacggc gaaggccgcc
    11521 cgccaggcct cgggcgtcgc gttgtcgagg ccgagccgcg gcgccacgcc ggcattgttg
    11581 accagcacgt cgagccggcc gaagcgctcg atcaccgccg cgacgatgcc gccgggcgct
    11641 gccggatcgg cgaggtcggc gacgatcacg aggccggcct cgggcggcaa ctgcccccgg
    11701 cggcgcacga ccagccccag ctccgcgcct tccttacgga acgcctcggc gatcgcctgc
    11761 ccgaagccgc cggcggcacc ggtcaggagc acgacgcgcc cggcgagcgg cccgctcaag
    11821 cctcgccttc cggctcgccg gccgggcgcc ggccgatccc gtgcatggtg gcgcgatgga
    11881 agctccgggc accggcggcg ctcagcacca gcagcgctat tcgccagccc tccgcggtcc
    11941 ggacgagacg atgatggtag ctggcccaga gctccccgtc cggacggccg tcgcagtagc
    12001 gctgccaagc gtagagatag cagtcgctcc gcgccgcgtc cggcccgtcg aacgcgatct
    12061 cgatattcga gacgtgatgg ctggtcgcgg cgaacagcgt ctcgaggccg cgcgccaccc
    12121 cctgctcgat cgccggccgg ccgacgaggt cggggaactc gggaccgtag tcgacgcgcg
    12181 catcggcggt gaagagccgc gccagcgcct tcggctcgtt gcggtcgaaa tgccggcaat
    12241 aggcgtggat cagcccggcg atcgcctggc gatccaggat ctcctgcaac acggcctcga
    12301 cctgcatgcg ctcccctcca cgacggtcgt ctcgacacgg gccgccagtt tgcgcacaac
    12361 cgtgcgcgaa acggaaggga gggcgaccat gggcatgctc gacggcaagg tggtgctgct
    12421 gaccggcgcc tcgaagggca tcggggcggc gacggcgggc gtgctgctgc gcgagggggc
    12481 cgcgctcgtc gcccagtacg gcggcgaccg cgccggtgcg gaggcagcga cagcgggcgg
    12541 cgatgcgctc ctggcccagg ccgatttcag ggacccgctc gccgtcgacc ggctctggcg
    12601 cgaggccctc gcctggcggg gccgggtcga cgtgctggtc aacaacgccg ccctcatgct
    12661 gacccatgcc ggcttcgacg ccggccaggc cgaatgggat gcagtctggg ccgagacgct
    12721 cgccgtcaat gtcacggccc ccgcccggct cacccgccac gcggtcaacc actttcgcga
    12781 ggccggcggc ggcgtgctgg tgaccgtctc gtcctgggcg gcccagcgcg gcagcggcaa
    12841 tcccgacctc gtcgcctact cggcgtcgaa ggccgcggtc aaggcgatga cccagaccat
    12901 cgcccgcggc ttcgccaggg agagcattct cgcctacatc gtcgccccgg gcgtcgtccg
    12961 cacccgcatg tcggaggact tcgccgccat gcagggcggc gaggacaagg tcacggcgac
    13021 cctcgccatg ggcgaatggg tgccacccgc cgacgtcgcc gaatgcacgg ccttcctcgc
    13081 cagcggccgc gcccgccacc tcagcggcgc gacgctcgac gtcaacgggg ccagctacat
    13141 ccgctgagcg aaatgggaca ccagggaacc aaacggagcc ctcggccgta gacagggttg
    13201 gagcccacga cagaaggaca tcccatgccc gagatcatgg acaagaccga agcccggcag
    13261 gccgagacca cgggccacgt ccgctacatc ctcgtcatct cgacaatcgc cgcgatcgtc
    13321 gtcctcggcg ccctcgtcct ctatttcgcc tgaccggcct cagcttcgct gcagccgcgc
    13381 ccgccgctcg agataccagt gatagagatg cggcagcagg ccgtgcgcct ccggtgcccc
    13441 gagcgccagc gctgcgcggt aggcccagac ggggtctgcc agatgctcgc gggcgatggc
    13501 gatcatgtcg gccttgcctt cggcgaggat cgcctcggcc tgctccgcct cggtgatgaa
    13561 gcccacggcc atggttgcga tgtcggcacc cttgcggatg gcatcggcga acggcacctg
    13621 gaagcccggg ccgggatgaa ccgtggcaag gctcgggctg ccggcgatgc cgcccgagga
    13681 gcagtcgacg agatcgatgc cgtgcgcctt cagccgcttc acgagctcga tggtgtcggc
    13741 gagctcgagc ccgccctcga ccccgtcctg cgccgatacc cggtaccaga ccggcaggtt
    13801 ctcgggccag gattcccgaa cgattcttgc cacttccagc ggcagcttca tgcggccttc
    13861 gagatcgccc ccataggcgt cgttgcgccg gttggtgagc ggcgagagga agctgtgcag
    13921 caggtagcca tgggcgccat gcagctcgac cacctcgaag ccggcggcca acgcccgctt
    13981 cgccgcgtcg cggaaggcgt gcctgatccg ggcgagatcg gcctcgtcca tggccttcgg
    14041 cgtgtgccag ccctcgcgca cggggatggc gctgggggca atggtctgcc acgggtcctc
    14101 gtcggccccg agcgggtgcg ccccttccca gggtctggcc gtgctcgcct tgcgcccggc
    14161 atggccgatc tggatcgccg gcacgcagcc gtagcgcttg taggccgccg tcacccgcgc
    14221 catcggggcg atctggccat cctcccagag cccggtgcag ccatgggtga tgcggccctc
    14281 cggcgtgacg ccggtggcct cgaccatgcc gaccgccacc ccgcccatgg cgaagcggac
    14341 atggtggtcg tagtgccagt cggtcatccg gccgtcgatc gcctggtact ggcacatcgg
    14401 cgagacgcag acccggttct tgaaggtcag gccgcgctgg gtgaaggggc ggaagagatg
    14461 gggtgtgcgg tccaccgtca ttccgctgcc tcggccggca ggacgcccag gtcctgcatc
    14521 acccgccgca gcttcgcgag ccggtcccgc ccctcctggc cgagcgaggc gaagctggtg
    14581 agcggcggcc gcacgtcgcc catggggtag ccgacgagcg ctgccagcgc cttcgagtag
    14641 cactggtggc cccaggtcgg atggccctcc gcgatggtgt gctcgatcac cgagatgtgg
    14701 cgctggatgg ccttcgcctc ctcgaccttg ccttcggcga ggagatcgca gatgcgcgac
    14761 gacggccccg ggatgtagtt gccgaacggg ttgacgtagc cgacggcacc caggaggtag
    14821 ctctcgacga tccgctcgcc cgcgaagacg ttcatcaccc cttccgtcgc ctcgacgatg
    14881 tcgaagaccc ggccgacatc catgctcgct tccttgatgt agcggatgtt gtcgaaggcc
    14941 cgggtgagct tcgccacgag cgccggcttc atgtcgacat tcgaggtgaa ggggttgttg
    15001 tagagcatga tcgggatggt gaccgcttcc gatatcgcct tgtaatagtt gaaaatctcg
    15061 tcctcggttg gtgtatagta atagggcggg acgatcatca gccccttcgc accgaggcgc
    15121 tccgcctcct tcgagtggcg cacggccgtc ggcgtgtgcg cgttcatcgt gccgaccagg
    15181 acggggatcc gcccggccgc ctggtcgacc gtcacctcga tcagccgcgt ccgctcggca
    15241 tcgctgaccg cgaggaactc gcccgacgtg ccgaggatga tgaggcccgg cacgccttcg
    15301 gcgatctgcc agtcgacgaa ccggcgctgc gccgcctcgt cgatgtcgct gccgtccgcc
    15361 gtgaagggcg tcacctgaac cgtgtagctg ccgcgaaact cgtgcccgtg catgtccgtc
    15421 tactttcgca ggtccgccca gccgtgctgg cggagaatgg cgtggctcgc caggaagtcc
    15481 tcgtccggcg tgctgtcggg caggatcttg ttggcgatga tctcgagcac cgtatagccc
    15541 tcgtagccga tgtcgcgaag cgctgcggcc accggcgcgg ggtcgacgat gccgcccggc
    15601 ttgccgagcc cgtgatgctt gaagacgtcg aagccgctgt cggagatgtg gacgttcctg
    15661 caccggggtc cgagtttcct gatcgccgcg gccgggtcct ccttgatgaa ggccgagttg
    15721 cagacgtcga agttcacgcc gaccgagggc tcgcccacga ggtccagcac cttcagcatg
    15781 tcgtcgacgg tcggcaggaa ggtgaagggc acgttctcga tcaggatctc gacgccggtc
    15841 cctttggcat gggccaccag ctccttcacg ccctcgacga accagttcag catccacgcg
    15901 tagggcgggt cgatcaggct gttcaccggc cccggaatgg cgaccacata ggggcagccc
    15961 cactcggccg cgaggtcgat atgctcgcgg taccggtcga gcgtgtagcg ccgcatcagc
    16021 gcgtcgggcg cgttcgggtt gttgtcgagc aaggggtagc agaaggagga gaggctgagc
    16081 ccgcgcgccg cgagctgctc ctggtacgcc ttccgctcgg tggcggtgag cagcggcggc
    16141 cagcagtgcg gggcgaagac gatcatctcg atcgcctcga agcccatgtc cgcgagatgc
    16201 agcgacgcct cgagcccatt ctccgtgtag atgtaggaga acgtgctcgc cgatagcttg
    16261 agatcggcca ttccttccct cctccagcag caccccgcaa gcggggcgcc ggcccgtaaa
    16321 caaccgttcg tatggcgcac aaacgatagt atgaccacag ccggactgca aagcgaaccg
    16381 cccttgcccc cgctccccct gaaggcactt cccgatcgga tcgacacggc cgtcatcggc
    16441 ggcggcatcg ccggctgcgc cgtggccttc gagctggcgc ggcgcggcgt cgaggtggcg
    16501 gtgctggagg ccggcgacct cgccgccatg gcctccggcg ccaactcggg cagcctgcac
    16561 gcccagattc cgatggagcc cttcctcctg gagggtgagg cctgggtccg gggcttcgcg
    16621 tccaccaccc gcctgctcgc cgcctcgatc gagctctggc gcggcctcga ggccctgctc
    16681 gaaacaccgc tcgaggtcag cctgaagggc ggcctgctcg tcgccgggag cgaggcgcag
    16741 ctcgcggcca tggcccgcaa ggtcgcgctc gagcgcagcc tcggcctcga ttcgcacctt
    16801 ctcggccaaa gcgagctggg cgaggtcgcc ccctatctcg ccccggggct cgcgggtgct
    16861 gccttctgcg cccaggaggg caaggcgagc ccgctcctcg ccggccccgc cttcgccgcc
    16921 gccgcgcggc ggaagggtgc ccaagtccac cccttcaccc cggttcgcac catctccgag
    16981 gccggctcgg gctaccgact cgaaacaacc aggggagtcc tcctcgcccg ccgcgtcgtc
    17041 atcgccaccg gcgcggatgc cggccgcacc gcctcgctgc tcggcctcga cctgccgctc
    17101 tggggcgagc cgatccaggc gagcatcacc gagcccgtcg ccgccatcat cccgcatctc
    17161 gtctatttcg ccggcgacaa gctgaccctc aagcagacgg cctcgggcgg cctgctcatc
    17221 ggcggcggct gggacgcccg gctcgacggc cggggccggc ccacggtcga cggcggcaat
    17281 ctcgcccaga acctcgccgt cgccgcccgg gtggtcccgg ccgtcgccag agcgaggctg
    17341 cttcggagct ggcccggcct cgtcaacgcc aatgccgact ggcggcccgt cctcggcccc
    17401 gtgcccggcc ggcccaatct gcatttcctc tgcttcccct ggatgggctt caccgccgcc
    17461 ccgatctgcg cccgcttggt tgcgcaggcc atcgttggcg agccgcccga cctgcccatc
    17521 gaggagttct ccatcgcccg ctacggcgcc gggtagccgt tcacccttct tgtcgatcgg
    17581 aaacacgccg ccgcatgcac cgcatcgtcg tcctcggcgg caagcccttt ttaggggccc
    17641 gaaatcctga aacccgacgg gccggcttca gtgccgcagg atgcgcgaga cgaactcgcg
    17701 gcagcggtcg gtccggggtg catcgaagac ctgcgcgggc gggccggttt cgacgatgct
    17761 gccctggtcc atcatcacca ccttggagga gacctcgcgc acgaagccca tctcgtggct
    17821 gacgatgagc atggtcatgc cgtcggcggc gagcacgcgg atggtgtcca gtacctcgcc
    17881 gaccagctcc gggtcgaggg ccgaggtgac ctcgtcgagc agcaggatct cgggcttgag
    17941 cgccagggcg cgggcgatgg cgacgcgctg ctgctggccg cccgagagct ggtccggata
    18001 gctctcgtgc ttctcggcga gccccacctt ggcgagcagg cgggccgcgt ccgcctcgac
    18061 ctcggggcgc ttccagccct tgatcctgat gggtgcaatc gtgacgttgc ccatggccgt
    18121 catgttctgg aagaggttgt attgctggaa gacgatggcg agccggtcac gggcgagctt
    18181 gaggctgcgg cggctggtgt agtcgatcgc ctcgccgtcg atccgcacgg caccgctggt
    18241 cggcggcagg aggcccacca ggaccctgag cagcgtgctc ttgccggacc ccgaggggcc
    18301 gatgaggctg acgatctcgc ccttctgcac ctcgagcgag acgtccctca gcaccggcgt
    18361 gtcggtgccc ggataggtgg cggtgaggtc agcgaccgcg agatggggca aggcgtctct
    18421 ccagccagcc gccgaggcgg ctcagcgggt acgagagggc gaagtacagc acgagcaccg
    18481 tgccgaacac cagcatggcc gagaagcccc gggtcgagag caccttgccg gcgaaggtca
    18541 gctcgaccac gccgagctgc gaggcgagcg ccgtgtcctt gatgaacatc accatgaagg
    18601 cgaaggcggg cggcaggatc acccgccagg cctgcggcag gacgacgtgg cgaagcgtct
    18661 gcagccggcc gaagttcatc accgcggccg tgtcccactg ggtctggtgg atcgaatcga
    18721 agccggcccg gatgatctcg gcgcagaagg ccgaggagat gacgcagatg gcgatcatcg
    18781 ccacgccgaa gagcgagacc gggaagcgaa agccctggat ggcgaagagc acgatgaaga
    18841 ggatgacgag gaaggggatc cgccggaagg tctcgacata gagcgtggcg gcgacacgca
    18901 gcggcagcca ggcgcggccc ggcgtccgcc tggcgaagtc gagcaggaag ccgagcacga
    18961 agcccaggct gacgccgatc agcgccatca gcagcgtgcg gcccatggcc tccaggagga
    19021 agaccatgtt gtagtaggtg aagaagcgcg gcgcctcgct gatgatgcga tccagcatca
    19081 gaagatcttc gctctgacgc gaaaggcgta gcggccgatc agcgccagca ccgccgaggc
    19141 gagaatcgtg atggcgatgt agatgagggc ggtgaccgag aagacctcga tggtgcggaa
    19201 attggtcgtg ctgatgttga tcgcccggcc ggtcaattcc tccacaccga agatcgaggc
    19261 catcgaggtg ccgagcacga gccagatgta gaagctcgag agcggggcga agagcgtctt
    19321 cgcgatatgc gggacgatga cgtagcggac gatctgggcg agcgacatgc ccagcacctc
    19381 ggccgcctcg atctcggtct gccggaccga gacgaagccg gcccgctgga tctcggtgag
    19441 ataggcgccg gaattgagga cgaggccgat gagcacggcg gtgaagggcg ccagcaggat
    19501 gccgacctcc ggcagggcgt agaagaggaa gaagatctgc acgagcgccg gcgtgttggt
    19561 gaagaagacg acgtagatat cgacgatccg atagaccagc cggccgccat agaccttgcc
    19621 gacggcaccg aagaggccga tcagcgcccc gccccagaat cccaggaagg ccagggccag
    19681 gctgagccag gccccctcga gcagataggg caggtgcttg agcgcctcgc cccattggaa
    19741 ggtgtaggtc attcgaggcc agcgcctgtc aggggccggc ggccgcagcc gccggcatca
    19801 atgaccgatc agaagaacgg gttcggctcg accttgacga gcatgtcggc accataccac
    19861 ttgcgccacg tctcgttgat gaagtcgctc gaatgcaggt cgtagagcag gatgttcagg
    19921 aagttgacga ggccggcatt gcccttctcg acaccgatgc cgcaatagcc gacgaagatc
    19981 gtgtccggca gcacccgcca gttcacgtcc ggatagttct cggtctgcgc catgaagaag
    20041 tcgatgttct cgaccagcgc gtcggcccgg ccctgggcca gcgagcgaac cgtgtcggcg
    20101 atgctgtcga ccagcagcat ctcggtctcg ggcaggttct ccttgttgaa gtcgaccggc
    20161 caggtgccgc ggatgttgac cagcgtgatg tccttggagt tgagatcctt ccagctctcg
    20221 gccgtcacct tgtcggtcgt cagcgccgcc atcgcctcgg tgtggagcgg cacggtgtag
    20281 tcgatcagct tggcgcgctc cgccgtccgg gtcagggccc cgagcgagat gtcgatggtg
    20341 ccggcggtga tgaagggcac gcgctgcggc gtctcggtcg gcacgaactc ggcctcgacg
    20401 ccgagcgcct cggcgatccg gttgccgacg tcgatgtcga agccctcgaa ctcgttggtg
    20461 gcgccatagg cgctcatcgg cgggaagttc gggttgacgc cgatcttgat ggtgccggac
    20521 gagatgatct cgtcgaggct cctggcgtcg gccgccgcga gcggcagcat ggccagtgcg
    20581 gtgatcgcaa cgaagcaggt tctgacatgc gcgagcatga ttgctggtcc tcccttgacc
    20641 ccggcacctt tgccggtgga tggctgtcga tcggttgccg aaggcgacaa catcaagcgt
    20701 gccgcccagc gtcaacggaa aagttcgcga gccgcacagc cgtgcgcaac tttcggcaag
    20761 gcccctaccc ggcgctctcg tcgatccccg cgatcgccgc gatcggcacc ggccggatcg
    20821 gcgcccgtgg cgccatcagg gcgaactcgt cgaccgcccg gccggtcgcc tcggcgatca
    20881 tggcggccat gaccggcgcg cagtagcggc cctggcagcg ccccatcccg gcccgcgtgc
    20941 gccgcttcag cgccccgatc gacgccgcac cgccggcgat cgcggcttgc agggtggcga
    21001 ggccgacctc ctcgcagcgg cagaccagcg tctcgggcgt ggccagggcg tggcccggca
    21061 agggggccgc gtaaaggcgc cagagcgctt tttggaagcg ccggccccgg gccagcgccg
    21121 cgcgcgcggc accgatcacc gccgcgtccg gaccgaagcc cagatcggcc gcggcccggg
    21181 cccccgcgat cgccccctgg gcctgcgcca cccgcgcccc gccgaggccg ccggcgtcgt
    21241 cggcgacgaa gacctccggc accgagcttc gacaatccgc gtcgcgcgcc acgcggagct
    21301 ggtcgaagcc cggatcgtgc tcgtggtcgc agcccagcgc ccgcgacagc tcgccctgcg
    21361 gcaggaagcc gtagccgctg gcgaccgtgt cgacagcgaa gcgccgctcg ctgcccgcaa
    21421 ccggtcggcc cgcctcgtcg atccgcgcga ccgccgcggc ctcgacactg ccctcgccct
    21481 cgatccgcac gagctggtga ccgtggagca aaggcacgcc gcgtcgcctc aggagccaga
    21541 gatcagaaaa gccgttgcgc acgaggtcgg gtgcggtgcg cgccatggcg agcatggccg
    21601 ccgggcgccg ccagggcgat ggcgccgtct cgaccagggc cgccacgtcg accccggcgc
    21661 gcagcagctc cgcggcgacc tgcaggttga gaggcccgtt gcccgcaacc aggacgcggc
    21721 tgccgggggc cacccgatag gcacgcagca gggtctggca ggcgccggtg gtcatcgccc
    21781 c
//
LOCUS       DAOTFI010000051        21726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2879_length_21726_cov_6.359743, whole genome shotgun sequence.
ACCESSION   DAOTFI010000051 DAOTFI010000000
VERSION     DAOTFI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21726
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2879_length_21726_cov_6.359743"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..723)
                     /locus_tag="P5558_07065"
     CDS             complement(223..723)
                     /locus_tag="P5558_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRY24104.1"
                     /translation="MMMAEMDKTDAAGRALPEAADVLATRFGPVPLDPDRQFRFRGGL
                     PGFPEAEHFQLDPIPDLDSDFMMLQAVGVPDIGFIVVVLPDDVPVIRRRDIEDVRAML
                     DIACGELMVLAVVTLVPTEKGVEKVVNLRAPLFVDARRKLGAQVVLSNPAYPLRHPLV
                     WSEAGN"
     gene            complement(848..1870)
                     /locus_tag="P5558_07070"
     CDS             complement(848..1870)
                     /locus_tag="P5558_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24105.1"
                     /translation="MSTETPAAAPANFDLFSFLAKFAPLIFLLVLMGVFALLEPRFLS
                     PLNLFNVMRQVSIYGLLAIGMTFVILTRGIDLSVGSLLAFAGLVAAAVAKGGFESRFA
                     VGTAAEAAGYGWFLAMLAALAVGVVCGGLQGLAITKLKVPPFVVTLGGMSAFRGAALL
                     FAGGGPVSGFDDAYRWWGQGYIGPIPVPVVIFAIAAIIAAIALRYTRFGRNVYAVGGN
                     PEAARLSGLDVDRITLSVYVIIGFMAGLGGFVLSARLNSAEAVAGLTYELTVIAGVVI
                     GGTSLFGGIGTIFGTVIGTLLIGVLLNGLVLNNVSSYVQQIIIGVIIVLAVAFDTFAK
                     SRRKRA"
     gene            complement(1875..3383)
                     /locus_tag="P5558_07075"
     CDS             complement(1875..3383)
                     /locus_tag="P5558_07075"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY24106.1"
                     /translation="MTGIRKSFPGVLAVDGVDLEVAPGEIHALLGENGAGKSTLLKIL
                     AGAQLPDEGSIELDGRPIEILSPHDAQEQGIVTIYQEFNLVPTLTVAENVFIGREPTR
                     GGWIDWGRMRRETRAIMDRVGLALSPDRLVSDLSVAEQQMVEIAKALAIQSRLIIMDE
                     PTSALAEGEVARLFAICRDLQAAGIAVIFVSHRLDEVKQLCDRLTVLRDGQLVGSAAV
                     ADVSIEDIIRMMVGRDVERLFKQRNVHRAGEPALRVEGLHASGDPRDTFATQLRGINL
                     EVRHGEIVGLAGLVGAGRTELARAVFGADRIDAGRVLVEGVPVQPGSPRRAIAAGIGL
                     VPEDRKQQALFLALAVRLNLSIAALDRLCGRLGFIDRGAETAMVEEYRERLNIRMPSA
                     DVAVGNLSGGNQQKVVLARWLALQPKVLIVDEPTRGIDVAAKAEVHQLLYELADSGIA
                     VLAISSELPEIMTISDRILTMREGRLTGELPIADATEEKLMQLMTMGRERAA"
     gene            complement(3449..4402)
                     /locus_tag="P5558_07080"
     CDS             complement(3449..4402)
                     /locus_tag="P5558_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY24107.1"
                     /translation="MPRSSRRLLLASAFALGLCLPVTGLHAQEIRVLTSVPSLGFPFF
                     VHMMKELQAEGAALGVATVESDGQNSTPKQTADVEAAVIQGLDGIVISPSDVNAMAPA
                     IQTAVENGVPVVTIDRRVDGVPGILAHVGADNVKGGEAQGEWIKANYPDGARIVNLQG
                     QPGASPAIDRNQGVHNALDGDAKYEFIVEQTANFARDQGLAVTESILAGLDSPPDVIV
                     AANDDMALGAVEAIKARGMGDQIAVIGFDALPEALAAVRDGVLAGTVEQFPGGQSRKA
                     MQIMVEHLKDGKAPEAEVVLLTPIVITADNIDEAERLGELN"
     gene            4571..4945
                     /gene="crcB"
                     /locus_tag="P5558_07085"
     CDS             4571..4945
                     /gene="crcB"
                     /locus_tag="P5558_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRY24108.1"
                     /translation="MALELLLVAFGGMLGGFGRALVSTLVAGLLGERLPWGTFAVNLS
                     GSLALGFLAGGLAGDPGPAPPLALFLATGLLGSYTTVSTFSLQTLFLLRERAFGRALL
                     NVAGSAGLCIAAASLGFVLAGP"
     gene            4942..5361
                     /locus_tag="P5558_07090"
     CDS             4942..5361
                     /locus_tag="P5558_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HRY24109.1"
                     /translation="MTRLLGLALATALGSGLGAVARFLLALALTGDGLPWATLLANVA
                     GSFLIGLAAEQSTRPGHVMADPFVRQFVMVGFCGGFTTFSLFSLEALLLVRAGVFELA
                     ALYVALSLALWLAGAWAGMTLGRRLVPGPLPEDRPAR"
     gene            5376..6581
                     /locus_tag="P5558_07095"
     CDS             5376..6581
                     /locus_tag="P5558_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY24110.1"
                     /translation="MMGDAVNETLYGRLGVPVIVNAKGAATRLSGGVLHPEVTAAMAE
                     ANAACVDMIDLHAAAAAEIAAATGAENGLATSGAAASLLLATAACMAGLDPAAMARLP
                     DARGLKREIVTIRSQRNAYDHALRGAGARLVEVGLADRFAGAGIRDAEAWELEAAIGP
                     KTAAVLWVANAKARPPLAEVVATAHRHDVPVIVDAAAELPPAAKLRAYIAAGADLVAF
                     SGGKGLGGPQASGILCGRHELVMSAALQMLDLDLRWGDFDPPPSFIDKALLPGLPRNG
                     IGRPCKADKGAIAGLLTALRRFLAESDAERTARWQAPLDHIRAELADLPLRLVEGGIP
                     RLELQLADAAAADRLYRLLLAHRPVIHVDHGELDAGRLHIAPNCLAPGDAERIVEALA
                     THWPRERRP"
     gene            6578..7330
                     /locus_tag="P5558_07100"
     CDS             6578..7330
                     /locus_tag="P5558_07100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24111.1"
                     /translation="MTGRVQDKIALVLGGARGIGAGIAERLAEDGARVVVGDRRAELG
                     EATARRIGGSFVTCDVGEPASIRAAVDDTVARFGRLDILAQNAGIFPMNDIVDIPVEE
                     WDQVLAVNLRGCFVATQAALRHMRKARYGRIILTSSITGPRVVPPGHAHYAASKAGIN
                     GFIKAAALENASFGITVNGVEPGNILTEGVQTERSPEYIASMVAAIPMGELGTPRDIG
                     NTVLFLASDEARYITGTTIIVDGGQLLPEGKG"
     gene            7327..8079
                     /locus_tag="P5558_07105"
     CDS             7327..8079
                     /locus_tag="P5558_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24112.1"
                     /translation="MSTGQPYLRVEHVSKHFGAVQALDDVSFEVHPGEVVALLGDNGA
                     GKSTLIKIISGTFGPSAGTLVFDGRPTRLESPADAKALGVETVYQDLSLCPNVDTVAN
                     FFMGREKTRKVLGVNVLDEPGMQAETEQALRDLGTHIPDVRTTVEHLSGGQRQAIELC
                     RFVHWGGKLVLLDEPFAALGVEQTRRGLEMIQRIKEQGVAVIVITHNVLHAFHVADRA
                     IVMRHGRVTGERRLGNATAEEIVALITGELAA"
     gene            8127..9971
                     /gene="ilvD"
                     /locus_tag="P5558_07110"
     CDS             8127..9971
                     /gene="ilvD"
                     /locus_tag="P5558_07110"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRY24113.1"
                     /translation="MPAYRSRTTTHGRNMAGARGLWRATGMKDGDFGKPIIAISNSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIAD
                     AVEYMVNAHCADALVCISNCDKITPGMLMAAMRLDIPTVFVSGGPMEAGKVVLEGKER
                     AIDLVDAMVAAADSSVSETDIATIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSLLATHADRRELFLEAGRVIVDLARRHYEGGEANLTPRQIAGFAAFENAMTLDIAMG
                     GSTNTVLHLLAAAHEAELDFTMADIDRLSRRVPVLCKVSPSVANVHMEDIHRAGGIMA
                     ILGELDRAGLIDRTVGCVHSTNLGAAIDRWDIARNGDDAVRTLYRAAPGGVPTQTAFS
                     QERRYKEVDLDREKGVIRQAANAFSKDGGLAVLYGNLATDGSIVKTTGVDESILTFAG
                     PAVVFESQDTAVQGILGGKVKPGDVVVIRYEGPRGGPGMQEMLYPTSYLKSKGLGKAC
                     ALVTDGRFSGGSSGLSIGHVSPEAAEGGWIALVENGDTIEIDIPNRRLHLAVDPAELD
                     RRQDAMQARGADAWKPAEHRPRKVTTALRAYAALTTSAARGAVRHVPD"
     gene            complement(9978..10565)
                     /locus_tag="P5558_07115"
     CDS             complement(9978..10565)
                     /locus_tag="P5558_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRY24114.1"
                     /translation="MTKTLPTLLAEIRACTVCGSSLPLGPRPVLQAGVSARLCIVGQA
                     PGRKVHETGIPWDDPSGGRLRAWLGLTETQFHDPTRVAIVPMGFCYPGRGASGDNPPR
                     PECAPLWHAPLMAHMPDIALTLLIGGYAQAWYLRGRRKASMGETIKAWREYLPIGYLP
                     LAHPSPRNQPWLVKNPWFEAELVPELRCLIAALEL"
     gene            complement(10594..11166)
                     /locus_tag="P5558_07120"
     CDS             complement(10594..11166)
                     /locus_tag="P5558_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRY24115.1"
                     /translation="MSDLQPPQRPDWPEGAVFTVGHSTLPIERFIAVLRAHGIELVVD
                     VRTVPRSRRNPQFNGDALAESLATVRVDYVQMRELGGLRRARADSPNTGWRNAGFRGY
                     ADHMQTEAFAAALDRLVDMSREKRLAMMCAEAVPWRCHRSLVADALLARGLPVVEILS
                     ESSHRMHELTPFARVDGTRLSYPPEQGSLL"
     gene            complement(11181..12710)
                     /locus_tag="P5558_07125"
     CDS             complement(11181..12710)
                     /locus_tag="P5558_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY24116.1"
                     /translation="MADETETTGPDLTRGIPEADLQDGRMLVGHVGDEAVLVARRGEA
                     VFAIGAACTHYGAPLADGLLVGETVRCPWHHACFSLRTGEALAAPAFRPASCWRVERQ
                     DGRIVVREKAPKISPAAARPGAESDARMVIIGGGAAGYAAAEMLRREGFAGAVVVVSA
                     DSSPPYDRPNLSKEFLSGKVPEKWLPLAGSRSYERKGIELRLDTRATRIDVPNHRVTL
                     DDGSALDYTKLLLATGAEPVRLDVRGAELPHVLTLRSLADCQALVDRATEGRRAVVVG
                     ASFIGLETAAALRQRGVDVSVVAPERRPMERLFGAAISDVVRDVHEEHGVVFHLGRKV
                     AGIDEKRVHLDNGAAIEADVVVFGIGVRPRLALAEQAGLAMDDGVLVDEHLETSVQGI
                     FAAGDIARWPDPYSRERLRVEHWVVAERQGQTAARNMLGAREAYTAVPFFWTMQHDLV
                     LHYHGHASTWDRIEQDGELAPRDATLRYIKGGRTLAVLTMGRDMASLEAELAMEQGRS
                     P"
     gene            12820..13872
                     /locus_tag="P5558_07130"
     CDS             12820..13872
                     /locus_tag="P5558_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY24117.1"
                     /translation="MPTTRRRLGLLAATAATVALLGASGARAEGETIYYMIPTLLDEF
                     QTESQAAIENVFGGMGYEVISLDAQNRADLQLNQLEDVINLQPDAIIMNAVDFDAIVP
                     GIEKARAAGIKVVNYDRQIRTTEFELTSVAGTVEIGRTAAAEAIRLLTERNGGPTGTV
                     LQILGDPGDNYTLDIQQGFEEVMAAEAPDVEIVTKAAMQWEATNAGRIFEDQLLVNDK
                     IDLVFAHAAHLTVPIVAIMEAQGRKPGEIMLMASNGAPVGLDNIRNGWQQVEVEQPLY
                     AQIYGLAMFMPKILRGEPLEPGMYDVTGLSAELTIEDWGPDLKVPGAAITPENVDDTR
                     FWGNLTPPTDPVTVVE"
     gene            13948..14949
                     /locus_tag="P5558_07135"
     CDS             13948..14949
                     /locus_tag="P5558_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24118.1"
                     /translation="MQARTTTPAGIPPRLIELVLDHLVWFILAVILLVCSLSIDRFFQ
                     VGIFLNIMQHATFVGILAIGLAFCIIAGHMDLSVESVMAFSAMLGAWLAADSASAAGF
                     ALPTIVVLATCLAFGALAGAFNAFLVVRLGINAFIVTLATYISIRGLGLILTGGRSIY
                     GLPDGFKGVAATSVLGVPLLVVILILAYVAFGLLLTRTRFGRYIYLVGGNPVAPFRAG
                     IDVQRMLFAAFILSGVLAAFAGFLLASRTNGATPNLGLGMLFEVFAAVVIGGVSLKGG
                     IGRLSGVFAGVLLLSTIDTAINVMGLDASYMQVIRGGLMLLAVLLDSLKSRIRKRYL"
     gene            14946..16631
                     /locus_tag="P5558_07140"
     CDS             14946..16631
                     /locus_tag="P5558_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24119.1"
                     /translation="MTTLAGFEPLSPAERSLLKSLESGQFDRLGDGSLPAPDAADRTI
                     RATLLRFLLVGGPDAPRPHEKGIRVSGALITGTLDLEGCRIPRDIGLVDCRFTAAPIM
                     RSAIVDTVFFDGSTLPGLVADLLEARGSIFLRSAAVSGSVVMPGARLGGELVCDGAAL
                     TQRDGLALDLERLEARGGVRLRGTTARGGIRLGGARLGGDLDAVGATLERPDALALDA
                     DGLIADGDVVLRLARVTGHARFIGARIDGDLDCSGADLRRPGGAVLTLDRAVIEGAFF
                     LRQQAAVDGTLSLVGASLGAVVDAPDSWPGPGDLLLNRCNYAALLDCPVDAATRLDWL
                     GRQEPTRWGDDFWPQPYEQLARVLAEMGHDTDAREVLIQKERLQRRARRARSSSGAVR
                     AILALNDLLLGITVRYGHQSLLAFAWLGLFWLAGAGLLATLAAQDALRPNVPIVLRSP
                     EWALCSTPTTKRLYLPSLDVTRPGLAAPGQSQLDCYLAQPEGQSYPKFNFWIYSLDAL
                     TPGLETGQQDYWAPDTRSTWGRIGRAYLYVHVLVGWALSLLAIAGFSGLVRSR"
     gene            16723..18255
                     /locus_tag="P5558_07145"
     CDS             16723..18255
                     /locus_tag="P5558_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HRY24120.1"
                     /translation="MPQTIDALLAPHAASAPAIGAPDRTWLDFAGLRELVAETRSSLA
                     ANGIGRNDRVAIVLPNGPEMAAAFVAVAASATTAPLNPAYRAEELDFYLSDLGAKALI
                     VGREEAGPAVAAAERHGCRILRLESDAGAPAGRFRLASDGAKVKAASPAAAGADDVAL
                     VLHTSGTTSRPKIVPLLQRNLAASARHIGQTLALGPADRCLNIMPLFHIHGLIAAVTS
                     SLAAGASVACSPGFNALRFFAWLEDVDPTWYTAVPTMHQAILTRADRNKDIIARIRLR
                     FIRSSSASLPPQVMAALEETFGCPVIESYGMTEATHQMASNPLPPAPRKPGSVGIAAG
                     PEVRIANAANGFVAEGGIGEVVISGPNVTPGYEANEAANAANFFQAEGKRWFRTGDQG
                     LMDEAGYLRLTGRLKEIINRGGEKVSPLEVDEVLMDHPAVQQVVTFAMPHAKLGEDVA
                     AAVVLREGKSADERSIRDFAATRLADFKVPRRVVILDEIPKGATGKLQRIGLAQKLGL
                     GT"
     gene            18252..19229
                     /locus_tag="P5558_07150"
     CDS             18252..19229
                     /locus_tag="P5558_07150"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRY24121.1"
                     /translation="MKICIYGAGAIGGLIGARLARTGIETSLVARGPHLAAMQANGLT
                     LVDGDERFTAAVTATDEPAKLGPQDYVIVTLKAHQVPGVVPLLQSLLGPETAVVMATN
                     GVPWWYFHGLEGPHEGHRLASVDPGDVQWSGIGPERVIGCVVYPAAEVEEPGVIRLVE
                     GDRFSLGEPSGERTPRIEALSKVLIETGLKAPIRPKIRDEIWIKLWGNLSFNPISALT
                     GATLDAICADPGTRAVARAMMLEAQATAEKLGVRFAIDVDRRIAGAAAVGAHKTSMLQ
                     DLERGRPMEIDALVTAVQEMARLTETASPTLDAVLALVQLRATVAGCYG"
     gene            19512..19721
                     /locus_tag="P5558_07155"
     CDS             19512..19721
                     /locus_tag="P5558_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY24122.1"
                     /translation="MSDIAKVFRSGRFQAIRLPEKYRMDTDEVEITREGEILILRPRK
                     RPGWSGLLAALDTVDAERFAECFAP"
     gene            complement(19932..20588)
                     /locus_tag="P5558_07160"
     CDS             complement(19932..20588)
                     /locus_tag="P5558_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24123.1"
                     /translation="MQFSMKLAMVFALAMSCAANVRAADIFILDDLDLDSVVAGRALT
                     GIQVTARTGVDDLNFSDAYLRLFLDGSAEGATSATCQPPCQATGTLLWVGAVPGSTLR
                     QEAERLGEFPQPDGVSYGAYGLGDEGINQIDAGAFGPTGSADARVKFFPVVQSDVVSA
                     TVGIGRGQTSGDGQVAVTGVVGEGRGIGQGYAIQTPFGDQLLFGIAIAVDPGAIVAAR
                     "
     gene            complement(20824..>21726)
                     /locus_tag="P5558_07165"
     CDS             complement(20824..>21726)
                     /locus_tag="P5558_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY24124.1"
                     /translation="LMTGSLTGLGLFHRKFDLPLDRVLHTTAPYFYRRPDPAMSEADF
                     TGYCVAELEALIAREGPDTIAAFIGEPALGTGGLVPPPAGYWAAIQEVLARHDILLVA
                     DEVVTGFGRLGSMFGSDHYGMRPDIITIAKGLTSAYAPLSGSIIGERMWDVLLQGTDE
                     NGPIGHGWTYSAHPIGAAAGIANLELIDELGLVQNAGETGAYLLAAMRDRLGGHPRVG
                     EIRGEGLLMAVEFVADRAERRFFDADEKVGARVVAALLAEGVVSRAMPEGDIAGFAPP
                     LCLTRGEADHIVDRMGKAVTTVLG"
BASE COUNT         3325 a         7767 c         7370 g         3264 t
ORIGIN      
        1 taccggagaa ccgccgatgg ccgccgtcag ctgcaccacc accagggaca cgaccgcggg
       61 tcaggcgcgc ggcggactat cgagcggcag atacggggcc gagacctgcc gggcctaatc
      121 gcgtgcggcg tggtgtgatc ggccgcttcg ggcgacctgc tcgtcgagac cggcaccggc
      181 gggtgtggct ctcttggccc gtgcctgcgg acgcggtgcg cgtcagttgc ccgcttcgct
      241 ccagaccagg gggtggcgca gggggtaggc ggggttggag aggacgacct gggcgccgag
      301 cttgcggcgg gcgtcgacga agaggggggc gcggagattc acgaccttct cgacgccctt
      361 ttcggtcggc accagggtga cgacggccag gaccatgagc tcgccgcagg cgatgtccag
      421 catggcgcgg acgtcctcga tgtcccggcg gcggatcacc ggcacgtcgt ccggcaggac
      481 gaccacgatg aagccgatat ccggcacgcc gaccgcctgc agcatcatga agtcgctgtc
      541 gaggtcgggg atcggatcga gctggaaatg ctccgcctcc gggaagccgg gcagcccgcc
      601 gcggaaccgg aactgccggt cggggtcgag cggcaccggg ccgaagcggg tggccaggac
      661 atcggcagcc tctggcagcg ccctcccggc agcgtccgtc ttgtccatct cggccatcat
      721 catgggcttc tcccgtgcac agagcccgtt gcacctccga gcgatcctag acgatcagcg
      781 ttaacgggcg gttaacagcc ggcgcaggtc cttgccctcc cgggcagccc tggcgatttc
      841 cggcgcgtca ggcgcgcttg cgccgggact tggcgaaagt gtcgaaggcg acggcgagca
      901 cgatgatgac gccgatgatg atctgctgga cgtaggagct gacattgttg agcacgaggc
      961 cgttgagcag cacgccgatc aggagggtgc cgatgacggt gccgaagatc gtgccgatgc
     1021 cgccgaagag cgaggtgccg ccgatcacca ccccggcgat caccgtgagc tcgtaggtga
     1081 gcccggctac ggcctcggcg gagttgagcc gggcggagag gacgaagccg ccgagcccgg
     1141 ccatgaagcc gatgatgacg tagaccgaga gcgtgatccg atcgacgtcg aggccggaga
     1201 ggcgggccgc ctcggggttg ccgccgaccg cgtagacatt gcggccgaag cgcgtgtagc
     1261 gcagcgcaat ggcggcgatg atggcggcga tggcgaagat gacgaccggc acggggatgg
     1321 ggccgatata gccctgcccc caccagcgat aggcgtcgtc gaagccgctg accgggccgc
     1381 cgccggcgaa gagcagggcg gcaccgcgaa aggccgacat gccgccgagc gtcacgacga
     1441 agggcggcac cttgagcttg gtgatggcga gcccctgcag gccgccgcag acgacgccga
     1501 cggcgagggc ggcgagcatc gcgaggaacc agccatagcc cgcggcctcg gcggcggtgc
     1561 cgacggcgaa gcggctctcg aagccgccct tggcgacggc ggcggcgacg aggccggcga
     1621 aggcgaggag cgagccgacc gagaggtcga tgccgcgggt caggatgacg aaggtcatgc
     1681 cgatggcgag caggccgtag atcgagacct ggcgcatgac gttgaagagg ttgaggggcg
     1741 agaggaagcg cggctcgagc agggcgaaga cgcccatcag cacgagcagg aagatcagcg
     1801 gcgcgaattt cgcgaggaag ctgaagaggt cgaaattggc gggggccgcg gccggcgttt
     1861 cggtcgacat cggctcaggc ggccctttcc cggcccatgg tcatgagctg catgagcttc
     1921 tcctcggtgg cgtcggcgat gggcagctcg ccggtcagcc ggccctcgcg catggtcagg
     1981 atgcggtcgc tgatggtcat gatctcgggc agctccgacg agatggcgag cacggcgatg
     2041 ccgctgtcgg cgagctcgta gaggagctgg tgcacctcgg ccttggcggc gacgtcgatg
     2101 ccgcgggtcg gctcgtcgac gatcaggacc ttgggctgca aggcgagcca gcgggccagc
     2161 accaccttct gctggttgcc gccggagagg ttgccgacgg cgacgtccgc gctcggcatc
     2221 ctgatgttga gccgctcgcg gtactcctcg accatcgccg tctcggcccc tcggtcgatg
     2281 aagccgagcc ggccgcagag gcgatcgagg gcggcgatcg agaggttgag ccggacggcg
     2341 agggcgagga agagcgcctg ctgcttcctg tcctccggca cgaggccgat gccggcggcg
     2401 atggcccggc gcggcgagcc cggctgcacc ggcacgccct cgacgaggac ccggcccgca
     2461 tcgatgcggt cggcgccgaa gacggcgcgg gcgagctcgg tgcggccggc gccgacgaga
     2521 ccggcgaggc cgacgatctc gccgtgccgg acctcgagat tgatgccgcg gagctgcgtc
     2581 gcgaaggtat cacgcgggtc gccactggca tggaggccct cgacgcgcaa agccggctcg
     2641 ccggcgcgat gcacgttgcg ctgcttgaac agccgctcga cgtcgcggcc gaccatcatc
     2701 cggatgatgt cctcgatcga gacgtcggcg acggcggcgc tgccgacgag ctggccgtcg
     2761 cggaggacgg ttagccggtc gcaaagctgc ttcacctcgt cgaggcggtg gctgacgaag
     2821 atcacggcga tgccggcggc ctgcaaatcc cggcagatgg cgaagagccg cgccacctcg
     2881 ccctcggcga gcgcggaggt cggctcgtcc atgatgatca ggcgggattg gatcgccagc
     2941 gccttggcga tctcgaccat ctgctgctcg gccaccgaga ggtcgctgac caggcggtcc
     3001 gggctcaatg ccaggccgac ccgatccatg atggcgcgcg tctcgcgccg catgcggccc
     3061 cagtcgatcc agccgccgcg agtcggctcg cggccgatga agacgttctc cgccacggtc
     3121 agcgtcggga ccaggttgaa ctcctggtag atcgtgacga taccctgctc ctgggcgtcg
     3181 tgcgggctca ggatctcgat cggccggccg tccagctcga tcgagccctc gtccgggagc
     3241 tgcgcgccgg ccaggatctt gagaagggtc gacttgccgg cgccgttctc gccgagcagc
     3301 gcatggatct cgcccggcgc cacctcgaga tcgacgccgt ccacggcgag gacgcccggg
     3361 aacgacttcc tgatgccggt catgcgcagg agcggcggac cgctcccgcg ctcgaccccg
     3421 gaggccaccg ctatatcaga cgggccgctc agttcagctc gccgagccgc tccgcctcgt
     3481 cgatattgtc ggccgtgatg acgatgggcg tgagcaggac cacctccgcc tcgggcgcct
     3541 ttccgtcctt caggtgctcg accatgatct gcatcgcctt gcggctctgc ccgcccggga
     3601 actgctcgac cgtgccggcg agcacaccgt cgcgcacggc ggcgagcgcc tcgggcaggg
     3661 cgtcgaagcc gatgaccgcg atctggtcgc ccatgccgcg ggccttgatc gcctccacgg
     3721 caccgagcgc catgtcgtca ttggcggcga cgatcacgtc gggcgggctg tcgaggccgg
     3781 cgaggatgct ctcggtcacg gcgaggccct ggtcgcgggc gaagttcgcg gtctgctcga
     3841 cgatgaactc gtacttggcg tcgccgtcga gggcgttgtg gacgccctgg ttgcggtcga
     3901 tcgccggcga ggcgccgggc tggccctgca ggttcacgat ccgggcgccg tccgggtagt
     3961 tcgccttgat ccactcgccc tgcgcctcgc cgcccttgac gttgtcggcg ccgacatggg
     4021 cgaggatgcc cggcacgccg tcgacccggc gatcgatggt cacgaccggc acgccgttct
     4081 cgaccgcggt ctggatcgcc ggcgccatgg cgttgacatc ggagggactg atgacgatgc
     4141 cgtcgagccc ctggatcacc gccgcctcga cgtcggcggt ctgcttgggg gtgctgttct
     4201 gcccgtccga ttcgaccgtg gcgacgccga gcgccgcccc ctcggcctgc agctccttca
     4261 tcatgtggac gaagaacgga aagccgaggc tcggcaccga ggtcagcacc cggatctcct
     4321 gggcgtgcag gccagtcacc ggcaggcaga gcccgagggc aaaagcgctg gcgagcagca
     4381 ggcgacggct ggaacgcggc atgtttcgac tccctgaatg gcgtgccctc gttcgggcac
     4441 ctgtcgtttg tgcgtgcgga caatcgggcg cggcgaaaag cctgtcaagc cgggccggcg
     4501 ttgcgcgatc cgggcttcgc cggcaagaac gaccggcaga tgcacaaggc ggatgggaga
     4561 acgcgcagcc gtggctctgg agctcctcct cgtggcattt ggcggcatgc tcggcggttt
     4621 cggccgggcg ctggtctcga ccctggtggc cgggctgctc ggcgagcggc tgccctgggg
     4681 caccttcgcc gtcaatctct cgggctcgct ggcgctgggc tttctcgccg gcggcctcgc
     4741 cggcgatccc ggccctgccc cgccccttgc gctcttcctc gcgaccgggc tgctcggcag
     4801 ctacacgacc gtctcgacct tcagcctgca aacgctcttt ctcctgcgcg agcgtgcctt
     4861 cggccgcgcc ctcctcaatg tcgcgggctc ggccgggctc tgcatcgccg ccgcctccct
     4921 cggcttcgtt ctggccggcc cgtgacccgg ctcctcggcc tcgcgcttgc caccgcgctc
     4981 ggcagcggcc tcggtgcggt cgcccggttc ctccttgccc tcgccctgac cggcgacggc
     5041 ctgccctggg cgacgctgct ggccaatgtc gccggctcct tcctcatcgg cctcgccgcc
     5101 gagcagagca caaggcccgg gcatgtcatg gccgacccgt tcgttcgcca gttcgtcatg
     5161 gtcggcttct gcggcggctt caccaccttc tccctcttca gcctcgaagc actcctgctc
     5221 gtccgggcgg gcgtcttcga gctggcggcg ctctacgtgg cgctttcact cgccctctgg
     5281 ctcgccggcg cctgggccgg catgaccctc ggccggaggc tcgtccccgg accccttccg
     5341 gaggaccgcc cggcccgata gaacggttgc ggcagatgat gggggacgca gtgaacgaaa
     5401 cgctctacgg ccggctcggc gtgccggtga tcgtcaatgc caagggtgcc gcgacgcgcc
     5461 tctcgggcgg cgtgctgcac cccgaggtga cggccgccat ggcggaagcc aacgctgcct
     5521 gcgtcgacat gatcgacctg cacgcggcgg cagcggccga aatcgccgcc gccaccgggg
     5581 ccgagaacgg cctcgccaca tcgggtgccg ccgccagcct gctgctcgcc accgccgcct
     5641 gcatggcggg gctcgatccc gcagccatgg cgcggctgcc cgacgcgcgc ggactgaagc
     5701 gcgagatcgt cacgattcgg agccagcgca acgcctacga ccacgcgctg cgcggcgccg
     5761 gggcgcggct ggtcgaggtc ggcctggccg accgcttcgc cggggccggc attcgcgacg
     5821 ccgaggcctg ggagctggag gccgccatcg gcccgaagac cgcggccgtg ctctgggtcg
     5881 ccaatgccaa agcccgcccg ccgctcgccg aggtggtggc caccgcacac cgccacgacg
     5941 tgccggtgat cgtcgatgcc gcggccgagc tgccgccggc ggcgaagctg cgggcctata
     6001 tcgcggccgg cgccgacctc gtcgccttca gcggcggcaa ggggctgggc gggccgcagg
     6061 cgtccggcat cctctgcggc cggcacgagc tcgtcatgtc ggcggcgctg cagatgctcg
     6121 acctcgacct gcgctggggc gacttcgacc cgccgccttc cttcatcgac aaggcgctgc
     6181 tgccgggcct gccgcgcaat ggcatcggcc ggccgtgcaa ggcggacaag ggggccatcg
     6241 ccggcctgct cacggccctt cgccgtttcc ttgccgagag cgacgccgag cgcaccgccc
     6301 gctggcaggc ccccctcgac cacatccggg ccgagctcgc cgacctgccg ctgcggctgg
     6361 tcgagggcgg catcccgagg ctcgagctgc agctcgctga tgcggccgcc gccgaccgcc
     6421 tctaccggct gctcctcgcc caccgaccgg tgatccatgt cgaccacggc gagctggacg
     6481 ccggccgcct ccacatcgcc cccaattgcc tcgcaccggg cgatgcggag cggatcgtcg
     6541 aggccctcgc gacacactgg cccagggaga gacgcccatg accggccgcg tccaggacaa
     6601 gatcgccctc gttctcggcg gtgcccgcgg catcggcgcc ggcatcgccg aacggctggc
     6661 cgaggacggg gccagggtcg tcgtcggcga ccgccgggcc gagctcggcg aagccacggc
     6721 aaggcgcatc ggcggcagct tcgtcacctg cgacgtcggc gagcccgcgt cgatccgggc
     6781 cgcggtcgac gacacggtgg cgcgcttcgg ccggctcgac atcctggcgc agaacgccgg
     6841 catcttcccg atgaacgaca tcgtcgacat cccggtcgag gaatgggacc aggtgctcgc
     6901 cgtcaatttg cgcggctgct tcgtcgcgac gcaggcagca ctccgccaca tgcgcaaggc
     6961 gcgctacggc cggatcatcc tcacctcgtc gatcacgggt ccccgagtcg tgccgccggg
     7021 ccatgcgcac tacgcggcca gcaaggccgg catcaacggt ttcatcaagg cggcggcgct
     7081 ggagaacgcc agcttcggca tcaccgtcaa cggcgtcgag cccggcaaca tcctgaccga
     7141 gggcgtgcag accgaacgct cgcccgagta catcgccagc atggtcgccg cgatccccat
     7201 gggcgagctc ggcacgccgc gcgacatcgg caacaccgtc ctcttcctcg cctccgacga
     7261 ggcccgctac atcaccggca ccaccatcat cgtcgatggc ggccagctgc tgccggaggg
     7321 caaggggtga gcaccggcca gccctatctc cgcgtcgagc atgtcagcaa gcattttggg
     7381 gcggtgcagg cgctcgacga tgtgagcttc gaggtccatc cgggcgaggt ggtggcgctg
     7441 ctcggtgaca acggggccgg caagtcgacg ctgatcaaga tcatctctgg cacgttcggg
     7501 ccgagtgcgg ggacactggt cttcgacggc cgaccgaccc ggctcgaaag cccggcggat
     7561 gccaaggcgc tgggggtgga gacggtctac caggacctct cgctctgccc caacgtcgac
     7621 acggtcgcca atttcttcat gggccgcgag aagaccagga aggtgctcgg cgtcaatgtg
     7681 ctggacgagc cggggatgca ggcggagacc gagcaggcgc tcagggatct cggcacgcat
     7741 atcccggacg tgcggaccac ggtcgagcat ctttcgggcg gccagcgcca ggcgatcgag
     7801 ctctgccgct tcgtccactg gggcggcaag ctggtgctgc tcgacgagcc cttcgcggcg
     7861 ctcggcgtcg agcagacgcg gcgcgggctc gagatgatcc agcggatcaa ggagcagggt
     7921 gtggcggtga tcgtcatcac ccacaacgtg ctgcacgcct tccacgtcgc cgaccgtgcc
     7981 atcgtcatgc gccacggccg ggttaccggc gagcgccggc tcggcaatgc gactgcggag
     8041 gagatcgtgg ccttgattac cggtgagctc gccgcttaag tctcgctcct ctccaacccg
     8101 aaccgccctc ccgactggaa cacgagatgc ccgcctatcg ctcccgcacg accacccacg
     8161 gccgcaacat ggccggcgcc cgcggcctct ggcgggccac cgggatgaag gacggcgatt
     8221 tcggcaagcc gatcatcgcc atctccaact ccttcaccca gttcgtgccc ggccatgtgc
     8281 atctcaagga cctgggccag ctcgtggcgc gcgagatcga ggcggcgggc ggggtcgcca
     8341 aggagttcaa caccatcgcc gtcgacgacg gcatcgccat gggccatgac ggcatgcttt
     8401 attcgctgcc gtcgcgcgag ctgatcgcgg atgcggtcga gtacatggtc aacgcgcatt
     8461 gcgccgatgc gctggtctgc atctccaatt gcgacaagat cacgcccggc atgctgatgg
     8521 cggccatgcg cctcgacatt ccgacggtct tcgtctctgg cgggccgatg gaggccggca
     8581 aggtggtgct cgagggcaag gagcgcgcca tcgacctggt cgacgccatg gtcgccgcgg
     8641 ccgacagctc ggtctcggag accgacatcg ccaccatcga gcgctccgcc tgcccgacct
     8701 gcggctcctg ctccggcatg ttcacggcca actcgatgaa ctgcctgacc gaagccctgg
     8761 gtctctcgct gccgggcaac ggctcgctgc tcgccaccca cgccgatcgg cgcgagctct
     8821 tcctcgaggc cgggcgggtc atcgtcgacc tcgcgcggcg ccactacgag ggtggtgagg
     8881 cgaacctgac gccgcggcag atcgccggct tcgccgcctt cgagaacgcc atgaccctcg
     8941 acatcgccat gggcgggtcg accaacaccg tgcttcatct gctggccgcg gcgcacgagg
     9001 cggagctcga cttcaccatg gccgacatcg accggctgtc gcgccgcgtg ccggtgctct
     9061 gcaaggtgtc gccttcggtg gccaatgtgc acatggagga catccaccgc gccggcggca
     9121 tcatggcgat cctgggcgag ctcgaccggg ccgggctcat cgaccgcacg gtcggctgcg
     9181 tgcacagcac caacctcggc gccgccatcg accgctggga catcgcccgc aacggcgacg
     9241 acgccgtccg cacgctctat cgcgcggcac cggggggcgt gccgacccag acagccttca
     9301 gccaggagcg ccgctacaag gaggtcgatc tcgaccgcga gaagggggtc atccgccagg
     9361 ccgccaacgc cttctcgaag gatggcggcc tcgccgtgct ctatggcaac ctcgccaccg
     9421 acggctcgat cgtgaagacc accggcgtcg acgaatcgat cctcaccttc gcgggcccgg
     9481 ccgtcgtctt cgagagccag gacaccgcgg tccagggcat cctcggcggc aaggtgaagc
     9541 cgggcgacgt ggtggtcatc cgctacgagg ggccgcgcgg cgggccgggc atgcaggaga
     9601 tgctgtatcc gacgagctat ctgaagtcga agggcctggg caaggcctgc gccctggtca
     9661 ccgacggccg cttctcgggc ggcagctcgg gcctctcgat cggccacgtc tcccccgaag
     9721 cggcggaagg cggctggatc gcgctggtcg agaacggtga caccatcgag atcgacatcc
     9781 cgaatcgccg gctccacctc gccgtcgacc cggccgagct ggaccgtcgc caggacgcca
     9841 tgcaggcccg tggcgccgac gcctggaaac ccgccgagca ccgcccgaga aaggtcacga
     9901 ccgcccttcg cgcctacgcc gccctgacca ccagcgccgc ccgcggtgcg gtccgccacg
     9961 tccccgacta ggagcaccta cagctcaagc gccgcgatca ggcaccgcag ctcgggcacc
    10021 agctccgcct cgaaccaggg gttcttcacc agccacggct ggttgcgcgg cgaggggtgg
    10081 gccagcggca ggtagccgat cggcaaatac tcccgccacg ccttgatggt ctcgcccatc
    10141 gaggccttgc ggcgaccgcg cagataccaa gcctgcgcgt agccaccgat cagcagggtc
    10201 agggcaatgt ccggcatatg cgccatgagc ggtgcgtgcc agagcggcgc gcattccggc
    10261 cgtggcgggt tgtcgccgga ggcacccctg cccggatagc agaagcccat cgggacgatg
    10321 gcgactcggg tcgggtcgtg gaactgcgtt tccgtcaggc cgagccaggc gcgcagccgg
    10381 ccgcccgacg ggtcgtccca ggggatgccg gtctcgtgca ccttgcggcc gggcgcctgg
    10441 ccgacgatgc agagccgcgc cgagaccccc gcctgcagga ccgggcgggg accgaggggc
    10501 aaggacgagc cgcagaccgt gcaggcccgg atttccgcca gcagcgtcgg cagtgttttg
    10561 gtcattcccg acccgactgg cgaacgggcc cggctacagc aatgacccct gctcgggcgg
    10621 gtagctgagc cgcgtgccgt ccacgcgtgc gaacggtgtc agctcgtgca tccggtggct
    10681 gctctccgac aggatctcga ccacgggcag gccgcgcgcc aagagcgcgt cggcgaccag
    10741 cgagcgatgg cagcgccacg gcacggcttc cgcgcacatc atggcgaggc gcttctcgcg
    10801 gctcatgtcg acgagccggt cgagggctgc cgcgaacgcc tctgtctgca tgtggtcggc
    10861 gtagccgcgg aagcccgcgt tgcgccagcc ggtgttgggc gaatccgccc tcgcccgccg
    10921 gaggccgccc agctcgcgca tttgcacgta gtcgacgcgc accgttgcga ggctctcggc
    10981 cagcgcatcg ccgttgaact gcgggttgcg gcgcgaacgc gggacggtac ggacgtcgac
    11041 gacgagctcg atgccgtggg cgcgcagcac ggcgatgaag cgctcgatcg gcaaggtcga
    11101 gtggccgacg gtgaagacgg ctccctcggg ccaatcgggt cgctgcggcg gctgaagatc
    11161 gctcattgaa tgctccggcg ctacggcgag cgcccctgtt ccatcgcgag ctcggcctcg
    11221 aggctcgcca tgtcccgccc catggtcagc acggccaggg tccgaccgcc cttgatgtag
    11281 cgcagcgtgg cgtcgcgcgg ggcgagctcg ccgtcctgct cgatcctatc ccaggtgctc
    11341 gcatggccgt gatagtggag cacgaggtca tgctgcatgg tccagaagaa cggcacggcc
    11401 gtgtacgctt cccgagcgcc cagcatgttg cgggcggcgg tctgcccctg ccgctccgcc
    11461 acgacccagt gctcgacacg caggcgctcg cggctgtagg ggtccggcca gcgcgcgatg
    11521 tcgcccgcgg cgaagatgcc ctgaacgctg gtttccagat gctcgtcgac gagcacgcca
    11581 tcgtccatgg cgaggccggc ctgctctgcg agagcgagcc gtggccgcac gccgatgcca
    11641 aagacgacga cgtccgcctc gatggcggcg ccgttgtcga gatgcacgcg cttttcgtcg
    11701 atcccggcaa ccttccggcc gaggtggaag acaacgccgt gctcctcgtg gacatccctg
    11761 accacatcac tgatggcggc accgaagagc cgctccatgg gccggcgctc cggtgccacg
    11821 acgctcacgt cgacgccgcg ctggcggagc gccgccgccg tctcaaggcc gatgaagctc
    11881 gccccgacga cgaccgcacg gcgcccttcc gtggcacggt cgacaagggc ctggcagtcg
    11941 gcgagcgacc gcagggtcag cacgtgcggc agctcggccc cgcgcacgtc gaggcggacc
    12001 ggctctgcgc ctgtcgcgag cagcagcttc gtgtagtcga gggcactgcc gtcatcgagg
    12061 gtgacgcgat ggttcggcac gtcgatccgg gtggcccgcg tgtcgagccg cagctcgatt
    12121 cccttccgtt cgtagctcct gctccccgcc agcggcaacc atttctccgg caccttgccc
    12181 gacaggaact ccttggatag gttgggtcga tcatagggcg gcgagctgtc cgcgctgacg
    12241 acgaccacag cacctgcgaa gccctcacgg cgcagcatct ccgccgcggc atagcccgcg
    12301 gcgccaccgc cgatgatgac catgcgcgcg tcggactcgg ctcctggccg cgcggcggca
    12361 gggctgatct ttggcgcctt ctcacgcacg acgatgcgac catcctgcct ctcgacgcgc
    12421 cagcaagagg cgggccggaa ggcgggtgcg gccagggctt cacccgtgcg caggctgaag
    12481 cacgcgtggt gccaggggca gcgcaccgtc tcccccacca ggagcccgtc ggccagggga
    12541 gccccgtagt gggtgcacgc ggcgccgatt gcgaagacgg cctcgccacg gcgtgccacc
    12601 aggacggctt cgtcgccgac atgcccgacc agcatccggc cgtcctgaag gtcggcctcg
    12661 ggaattcccc gggtcagatc gggacccgtc gtctcggtct cgtcggccac ggcgtctttc
    12721 ctccgggcag ttcgtcgcct tcggaccaac cgttgccagc accgctcgtt cccctctagt
    12781 ctctgtgccg gagaaggctg aaaacagagg ggaacccaca tgcccacgac acgccgccgc
    12841 ctcggactgc tcgccgccac cgccgccacg gtcgccctgc tgggcgcttc gggcgccagg
    12901 gccgagggcg agaccatcta ctacatgatc ccgaccctgc tcgacgagtt ccagaccgag
    12961 agccaggcgg cgatcgagaa cgtcttcggc ggcatgggct acgaggtgat ctcgctcgac
    13021 gcccagaatc gcgccgacct gcagctcaac cagctcgagg acgtgatcaa cctccagccc
    13081 gacgccatca tcatgaacgc ggtcgatttc gacgccatcg tgccgggcat cgagaaggcg
    13141 cgggccgccg gcatcaaggt ggtcaactac gaccggcaga tccgcacgac cgagttcgag
    13201 ctgacctcgg tcgcgggcac ggtcgagatc ggccgcacgg cagccgccga ggccattcgc
    13261 ctgctcaccg agcgcaatgg cgggccgacc ggcacggtcc tgcagatcct gggcgatccc
    13321 ggcgacaact acaccctcga catccagcag ggcttcgagg aggtgatggc ggccgaggcg
    13381 ccggacgtgg agatcgtcac caaggcggcg atgcagtggg aggcgaccaa tgccggccgc
    13441 atcttcgagg accagctcct ggtcaacgac aagatcgacc tcgtcttcgc ccacgccgcc
    13501 cacctgaccg tgccgatcgt cgccatcatg gaggcccagg gcaggaagcc cggcgagatc
    13561 atgctgatgg cctcgaacgg tgctccggtc ggcctcgaca acatcaggaa cggctggcag
    13621 caggtcgagg tcgagcagcc gctctacgcg cagatctacg gcctcgccat gttcatgccg
    13681 aagatcctgc ggggcgagcc gctcgagccc ggcatgtatg acgtcaccgg cctttcggcc
    13741 gagctgacga tcgaggactg gggtcccgac ctcaaggtgc cgggggcggc gatcacgcca
    13801 gagaatgtcg acgacacccg cttctggggc aacctcaccc cgcccacgga cccggtcacg
    13861 gtcgtcgaat aggccagaag ctcacaacca cgcgcacccg ggcggcagcc ctgccgtccg
    13921 ggtgcgccgc gttcgccggg gatcatcatg caggccagga caacgacgcc ggcgggcatc
    13981 ccgccccgcc tgatcgagct cgttctcgat cacctcgtct ggttcatcct ggcggtcatc
    14041 ctgctcgtct gctcgctctc gatcgaccgc ttcttccagg tcggcatctt cctcaacatc
    14101 atgcagcatg cgaccttcgt cggcatcctc gccatcggtc tcgcgttctg catcatcgcc
    14161 gggcacatgg acctctcggt cgaatcggtc atggcgttct cggccatgct cggcgcctgg
    14221 ctcgccgccg acagcgcctc ggccgccggc ttcgcgttgc cgaccatcgt cgtgctcgcc
    14281 acctgcctcg ccttcggggc gctggccggc gccttcaacg cctttctcgt cgtcaggctc
    14341 ggcatcaacg ccttcatcgt gacactcgcc acctacatct cgatccgcgg cctcggcctg
    14401 atcctgacgg gcggccggtc gatctacggc ctgcctgacg gcttcaaggg ggtggcggcc
    14461 accagcgtcc tcggcgtgcc gctcctcgtc gtcatcctta tcctggccta tgtcgccttc
    14521 ggcctgctgc tcacgaggac gcgtttcggc cgctacatct acctcgtcgg cggcaacccg
    14581 gtggcaccct tccgcgccgg catcgacgtg cagaggatgc tgttcgcggc cttcatcctc
    14641 tccggcgtgc tggcggcctt cgccggcttc ctgctggcgt cgcgcaccaa tggcgccacg
    14701 cccaatctcg gcctcggcat gctcttcgag gtcttcgccg cggtggtcat cggcggcgtc
    14761 tcgctcaagg gcggcatcgg ccgcctctcc ggcgtcttcg ccggcgtgct gctgctttcg
    14821 accatcgaca ccgccatcaa cgtgatgggc ctcgatgcct cctacatgca ggtgatccgg
    14881 ggcgggctga tgctgctcgc ggtgctgctc gacagcctca agtcgcggat caggaagcgc
    14941 tacctttgac gacgctcgcc ggcttcgaac ccctgtcgcc ggccgagcgg tcgctgctga
    15001 agagcctgga aagcggccag ttcgaccggc tcggcgatgg cagcctgccg gcgccggacg
    15061 cggccgaccg cacgatccgc gccaccctgc tccgcttcct cctggttggc ggccccgacg
    15121 cgccgcggcc gcacgagaag ggcatccggg tcagcggcgc gctgatcacc ggaacgctcg
    15181 atctcgaagg ctgccgcatc ccgcgcgaca tcggcctcgt agactgtcgc ttcaccgcgg
    15241 caccgatcat gcgatccgcc atcgtcgaca ccgtcttctt cgacggctcg acgctgcccg
    15301 gcctcgtcgc cgacctgctc gaggcgcgcg gctcgatctt cctgcgcagc gccgcggtca
    15361 gcggcagcgt ggtcatgccc ggcgccaggc tcggcggcga gctggtctgc gacggggccg
    15421 cgctgacgca gcgggacggg ctggcgctcg acctcgagcg gctcgaggcg cgcggcggcg
    15481 tgcgcctgcg cggcaccacg gcgcggggcg gcatccgcct gggcggcgcg cggctgggcg
    15541 gcgacctcga cgccgtcggc gccacgctcg agcggcccga cgccctggcc ctcgacgcgg
    15601 acgggctcat cgccgacggc gacgtcgtgc tgcggctggc acgggtgacg ggccacgccc
    15661 ggttcatcgg cgcgcggatc gacggcgacc tcgactgcag cggcgccgac ctccggcggc
    15721 ccggcggcgc cgtcctcacg ctcgatcgcg cggtgatcga gggcgccttc ttccttcgcc
    15781 agcaggcggc ggtcgacggc acgctcagcc tcgtcggcgc ctcgctgggc gccgtggtcg
    15841 acgcgcccga cagctggccc gggccgggcg acctcctgct caaccgctgc aactacgccg
    15901 ccctcctcga ctgcccggtc gacgccgcga cccgcctcga ctggctcggc cgccaggagc
    15961 ccacccgctg gggcgacgac ttctggccgc agccgtatga gcagctcgcc agggttctgg
    16021 ccgagatggg ccacgacacc gacgcccgcg aggtgctgat ccagaaggag cggctgcagc
    16081 gccgcgcccg ccgcgcccgc agctccagcg gcgcggtccg ggccatcctc gccctcaacg
    16141 accttctgct gggcatcacg gtgcgctacg ggcaccagtc cctgctcgcc ttcgcctggc
    16201 tcggcctctt ctggctcgcc ggcgccggcc tcctcgccac gctcgcggcg caggacgcgc
    16261 tgcgtcccaa cgtcccgatc gtgctccgct cacccgaatg ggccctctgc tcgacgccga
    16321 ccacaaagcg gctctatttg ccatcgctcg acgtgacccg accggggctg gcggcgccgg
    16381 gccagtcgca gctcgactgc tacctcgccc agcccgaggg tcagagctac ccgaaattca
    16441 acttctggat ctactcgctc gatgccctga cccccggcct cgagaccggc cagcaggact
    16501 attgggcgcc cgacacgcgc agcacctggg gccgcatcgg tcgcgcgtat ctctacgtcc
    16561 acgtcctcgt cggctgggcg ttgagcctgc tcgcgatcgc cggcttctcg ggcctcgtcc
    16621 gctcacgctg aaagcctgcc tcgggcgagg caccctttcc atcgcggggc cttcggggct
    16681 atctggccag aagcgcacgc tccaccaccg ccggaacgcc ccatgcccca gaccatcgac
    16741 gccctcctcg ccccccatgc cgcaagcgcc ccggccatcg gcgcgccgga ccggacgtgg
    16801 ctcgacttcg ccgggctgcg cgagctcgtg gccgagaccc gctccagcct cgccgccaac
    16861 ggcatcggcc gcaacgaccg ggtcgccatc gtcctgccga acggcccgga gatggccgcg
    16921 gccttcgtcg cggtcgccgc cagcgccacc acggccccgc tcaacccggc ctaccgggcc
    16981 gaggagctcg atttctatct ctcggacctc ggggcgaagg cgctgatcgt cggccgggag
    17041 gaggccggcc cggcggtcgc cgccgccgag cgccatggct gccgcatcct gcggctggag
    17101 agcgatgccg gggcgccggc cggtcgcttc cgcctcgcga gcgacggggc caaggtgaag
    17161 gcggcgagcc cggccgccgc cggggccgac gacgtggcgc tggtgctgca cacgtccggc
    17221 acgacgtcgc ggccgaagat cgttccgctc ctgcagcgca acctcgcggc ctcggcccgg
    17281 catatcggcc agacgctggc gctcggcccc gccgatcgct gcctcaacat catgccgctc
    17341 ttccacatcc acggcctgat cgcggcggtc accagctcgc tcgccgccgg cgcctcggtc
    17401 gcctgctcgc ccggcttcaa cgcccttcgc ttcttcgcct ggctcgagga cgtcgacccc
    17461 acctggtaca cggccgtgcc gaccatgcac caggcgatcc tgacgcgcgc cgaccgcaac
    17521 aaggacatca tcgccaggat aaggctccgc ttcatccgct cctcctcggc ctcgctgccg
    17581 ccccaggtca tggcggcgct cgaggagacc ttcggctgcc cggtgatcga gagctacggc
    17641 atgaccgagg cgacccacca gatggcctcg aacccgctgc ccccggcacc gcgcaagccg
    17701 ggctcggtcg gcatcgccgc cgggccggag gtgcggatcg ccaacgcggc gaacggcttc
    17761 gtcgcagagg gcggcatcgg cgaggtggtt atctccggcc ccaacgtcac gcccggctac
    17821 gaggccaacg aggcggccaa cgccgcgaac ttcttccagg cggaaggcaa gcgctggttt
    17881 cgcacgggcg accaggggct catggacgag gccggctatc tgcgcctcac cggccggctc
    17941 aaggagatca tcaaccgggg cggcgagaag gtgagcccgc tcgaggtgga cgaggtgctg
    18001 atggaccacc cggccgtcca gcaggtggtc accttcgcca tgccgcacgc gaagctcggc
    18061 gaggacgtcg ccgccgccgt ggtcctgcgg gaaggcaaga gcgccgacga gcgctcgatc
    18121 cgcgatttcg ccgccacgcg cctcgccgac ttcaaggtgc cgcgccgggt cgtcatcctc
    18181 gacgagatcc ccaagggtgc caccggcaag ctgcagcgca tcggcctcgc ccagaagctc
    18241 ggcctcggca cgtgaagatc tgcatttatg gcgcgggggc gatcggcggc ctcatcggcg
    18301 cccgcctcgc ccgaacggga atcgagacga gcctcgtcgc ccgcggcccg catctcgccg
    18361 ccatgcaggc gaacggcctg acgctggtcg atggcgacga gcgcttcacc gcggccgtca
    18421 ccgccaccga cgagccggcg aagctcgggc cgcaggacta cgtgatcgtc accctcaagg
    18481 cgcaccaggt gccgggcgtg gtgcctttgc tgcagtcgct gctcggcccc gagaccgccg
    18541 tggtcatggc aaccaacggc gtgccctggt ggtatttcca cgggctggaa ggcccgcatg
    18601 aaggccaccg cctagccagc gtcgatccgg gcgacgtgca atggtccggc atcgggccgg
    18661 agcgcgtcat cggctgtgtc gtctacccgg cggccgaggt ggaggagccc ggtgtcatcc
    18721 gcctcgtcga gggcgaccgg ttctcgctcg gcgagccttc gggcgagcgc acgccccgca
    18781 tcgaggcgct ctcgaaggtt ctgatcgaga ccggcctcaa ggcgccgatc cggccgaaga
    18841 tccgggacga gatctggata aagctctggg gcaacctctc cttcaacccg atcagcgcgc
    18901 ttaccggtgc cacgctcgac gccatctgcg ccgatcccgg cacccgtgcc gtcgcccgtg
    18961 cgatgatgct cgaggcccag gccacggccg agaagctggg cgtccgcttc gccatcgatg
    19021 tcgacaggcg catcgccggc gccgccgccg tcggcgccca caagacatcg atgctgcagg
    19081 acctcgagcg cggccgcccg atggagatcg acgccctggt caccgccgtc caggagatgg
    19141 cgcggctgac cgagaccgcc tcgcccaccc tggatgcggt tctggcgctg gtccagctac
    19201 gggcgacggt ggcggggtgc tatggatgac caaacgggcg gaaactccat ccgccgatgg
    19261 atgaggatga cacgaatggc gaaaggcgat gaccagggaa tcgacccggc gcccgccaag
    19321 ggacttgtcg gagctgcctc ccaaagtgcg tgaggagtcc gcgctcgccg cattgcgcgc
    19381 cgatcttcag ctcgctatcg accaactcga ccggggcgaa ggcattccag ccgaggaggt
    19441 ctttcgcagc ctccgggcat gcggcgccac gcgcgacgag caacactttg tcgcgagggg
    19501 caggaggatc gatgagcgac attgccaagg tcttcagatc aggtcgcttc caggctattc
    19561 gcctgcccga gaaatatcgc atggacaccg acgaggtcga gatcacccgc gagggtgaaa
    19621 tcctgatcct gcggccgcgc aagcggccgg gctggtcagg gctgctggcg gcgctcgaca
    19681 ccgtcgacgc ggagcgcttc gccgagtgct ttgcaccatg acacgcaaaa cccggggcgg
    19741 ttcggcgcga cctcgagaag gtgcgccgga accttcaccc ccggcgtcgt cgtctaaaat
    19801 ctggtgcggt ggtccaagct gctgacgggg aggtgaccga cggcatgaac tgtcagacct
    19861 gccttcgtca agtgacgaca atactggttt ggaatgtctg gcagcctttt ttggcacttc
    19921 aatgctgccg atcagcgcgc cgccacaatg gcgcccgggt ccacggctat cgcgatgccg
    19981 aaaagaagct gatccccgaa tggcgtctga atggcatatc cctgaccgat gcccctgcct
    20041 tcgcccacga cgccggttac cgcgacctgg ccgtcaccgc tcgtttgccc tcgaccgata
    20101 ccgacagtgg cggaaacgac atcgctctgc accacgggaa agaacttgac gcgggcgtct
    20161 gcgctgcccg taggaccaaa tgccccagcg tcgatctgat tgatcccttc gtcacccaag
    20221 ccgtacgcgc catatgacac accatcaggc tgcggaaact ctcccaaccg ctcggcctcc
    20281 tgacgcaatg tggaacccgg caccgcgcct acccatagca gtgtcccggt agcttgacac
    20341 ggaggctggc aggttgccga cgtggcacct tcagcggatc cgtccaggaa aagacgtaga
    20401 taggcatcgc tgaaattgag gtcatcaact ccagtacgtg ctgtcacttg gatgccggtg
    20461 agggcacggc cagcgacaac cgaatcgaga tctaaatcat caagtataaa tatatccgca
    20521 gctcggacat ttgcagcgca tgacattgcc aaagcaaaaa ccatcgcgag cttcatcgag
    20581 aactgcattt ctacctccca ataccatcaa aagttgccgc atcttaacta cacgcgcaat
    20641 ggcacaatcg atgagagcgc acattttttc ccacgcccgt cagcatgaat tgcgaatttt
    20701 cctttgaagg tcaagagtga attcgccatt cgcgctcgtc gatgacgatg ttggcatcga
    20761 gggcttgcat tgggtcgatt ggctgacctg aagtggtcgg ccaaccgcca caactccgaa
    20821 cccctacccc aataccgtcg tcaccgcctt ccccatccga tcgacgatat ggtccgcctc
    20881 cccccgcgtc aggcagagcg gcggggcgaa gccggcgatg tcgccttcgg gcatggcccg
    20941 cgagacgacg ccttcggcga ggagggcggc caccacccgg gcacccacct tctcgtcggc
    21001 atcgaagaag cgccgctcgg cgcggtcggc gacgaattcg acggccatga gcaggccctc
    21061 gccccggatc tcgccgacac gggggtggcc gcccagccgg tcgcgcatgg cggcgaggag
    21121 ataggcgccc gtctcgccgg cgttctgcac gaggccgagc tcgtcgatca gctcgagatt
    21181 ggcgatgccg gcggcggcac cgatgggatg ggccgagtag gtccagccat ggccaatggg
    21241 gccgttctcg tcagtgccct gcagcaggac atcccacatc cgctcgccga tgatcgagcc
    21301 ggagagcggt gcgtaggccg aggtcaggcc cttggcgatg gtgatgatgt cgggccgcat
    21361 gccgtagtgg tccgagccga acatcgagcc cagccgcccg aagccggtca ccacctcatc
    21421 ggcgacgagg aggatgtcgt ggcgggccag gacctcctgg atggcggccc agtagcccgc
    21481 gggcggcggc acgaggccac cggtgccgag cgccggctcg ccgatgaagg cggcgatggt
    21541 gtccggccct tccctggcga tcagcgcctc gagctcggcc acgcaatatc ctgtgaaatc
    21601 ggcctcggac atggcggggt cgggccggcg ataaaaatag ggggcggtgg tatgcaggac
    21661 ccggtcgagc ggcaggtcga acttccggtg gaagaggccg agcccggtga gcgagccggt
    21721 catcag
//
LOCUS       DAOTFI010000052        21640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2894_length_21640_cov_6.365207, whole genome shotgun sequence.
ACCESSION   DAOTFI010000052 DAOTFI010000000
VERSION     DAOTFI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21640
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2894_length_21640_cov_6.365207"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..2288
                     /locus_tag="P5558_07170"
     CDS             81..2288
                     /locus_tag="P5558_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRY24125.1"
                     /translation="MAASQVLSGIVDRVVVQNPTTGFAVLRVKVPGRSESITVVGAVA
                     NSRPGLVVRAEGDWQDDPAWGHQFAATRIILLAPASVDGIRAYLASGAIKGVGEALAQ
                     RIVEHFGADLRHVLDEAPERLREVPGIGARTLQRITEVWRNEGENRDILLFLESQGIR
                     PARARRILEAYSGRAIEQLLADPYALARDIRGIGFDTADLLADQLGITADAPVRRRAA
                     LLEVLRGNAERGHSAMHLERALNEAATLSGQSPTAIAEVADGLRHERRIVERTTEAGT
                     FLLLPELDAAEATIVRRLAILSAGPVPWDRTALGDWEAVVRVRLALELSPSQARALAV
                     ILTSRVAILTGGPGTGKTSLVRACVACLERQQVRLALAAPTGRAARRLFESTGHEATT
                     IHRLLEADIQRGFARNAERPLDLDLLILDEVSMVDLPLMEGVVAALPDRAALLLVGDR
                     DQLPPVGPGQVLADLIDSERFPVVALTEIFRQAEASGIVRGAHRVIRGELPRFGTDEA
                     GDCFGIRVRDAADARAKLIELVHHRMPERFDLDPVADIQVLVPVNRGPVGTRDLSSTL
                     QKVLNPSPEMAFERFGVRFGIGDKVMQIENDHSREVYNGDLGIIQKIDSGARLMEVRF
                     DDRLVVYGFDSVDQLVSAYAITVHKAQGSEYPAVVLLLLHEHGRMLRRQLLYTAMTRA
                     RRLLVVLAQGSALERAVATPEPARLSLLGHRLATEWATGPAGERTSPIQRTNP"
     gene            2285..3085
                     /locus_tag="P5558_07175"
     CDS             2285..3085
                     /locus_tag="P5558_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HRY24126.1"
                     /translation="MTDEDLDRRLDAGVPILHAAGRLALDFFQRRGELVIEHKGLQDL
                     VSIADQTVERAIRDELGRLFPGDAIIGEEDGAGAGYAAAPGVWVIDPIDGTSNFLRGM
                     PYWCCVMAYVVDGTTEIGLTYDPIHDEMFACRRGAEATRNRQPIEVSSRPVAEACVGL
                     SFNFKQDAREYAEMVERLNARSFDHRRSGSSALHLCHVADGRLDGAINRHCMSWDVLA
                     GLALVEAAGGVATHYTRGASLIEPRAVLATTPALRAVVEEAAGFPLDS"
     gene            complement(3110..4333)
                     /locus_tag="P5558_07180"
     CDS             complement(3110..4333)
                     /locus_tag="P5558_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRY24127.1"
                     /translation="MADPGPKTESGRPTEARPVFDWADPFRLGDQLDESERLIRDAAA
                     DYCQNGLMPRVLEANRHEVFDQEIMTELGALGFLGSTLPEAYGGADASYVAYGLIARE
                     VERVDSGYRSAMSVQSSLVMHPIHAYGDEAQRRKFLPKLAKGELVGCFGLTEPDHGSD
                     PGGMTTKARKAPGGFRLSGAKTWITNSPIADLAIVWAKTDDGVIRGFIVERDMQGFTT
                     PKIEGKFSLRASITGMIQLDDVLVPEANLLPNVKGLKGPFGCLNRARYGIAWGTMGAA
                     EFCWHAARGYTLERQQFGRPLAANQLIQKKLADMQTEITLGLQAALRVGRLMDEGAMA
                     PEMISLIKRNNCGKALDIARTARDMHGGNGISDEYHVIRHVMNLEAVNTYEGTHDIHA
                     LILGRAQTGLQAFSG"
     gene            complement(4638..5189)
                     /locus_tag="P5558_07185"
     CDS             complement(4638..5189)
                     /locus_tag="P5558_07185"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRY24128.1"
                     /translation="MAPTHSLQKSTRRWFITGAAAAVGLAASPLEAATQPRRLLLHNL
                     HTKEDLDVVYRQGQEFDRRALTSLNYFLRDWRREEVLPIDPRLYDIMAAIADRLGQAP
                     RFEIISGYRSPQTNAMLRRAGGGAAKRSLHMFGRAIDLRLEGARLRDVREVARQLERG
                     GVGYYPKSQFVHIDTGKVRTWNG"
     gene            complement(5256..6104)
                     /locus_tag="P5558_07190"
     CDS             complement(5256..6104)
                     /locus_tag="P5558_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRY24129.1"
                     /translation="MELARSLDLDAYKPAEIARLIERSGVAKARLGTLQTLTLGLLAG
                     AFIAMGSMFYTLVMTGDGLGFGPARLLGGLVFSLGLILVVIAGAELFTGNNLIVMAWV
                     DRRITLLDLARNWTLVYVANFIGAVGTAVLLTLSGTLALGDGAVRETAFAIALAKTGL
                     VWHEALFRGLLCNALVCLAVWLTFAARDVTGKILAILLPIAAFVALGFEHSVANMYFI
                     PLAWMNGAPGVGIDGLLANLVPVTVGNIIGGGGFVGLTYWLCYRPSPASTSHPDEGIR
                     GTTTED"
     gene            complement(6134..7960)
                     /locus_tag="P5558_07195"
     CDS             complement(6134..7960)
                     /locus_tag="P5558_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24130.1"
                     /translation="MAAATLLSLRRASLSLGGKRLFDDLELDIAAGAAIGLVGRNGSG
                     KSTLLKVLAGQIELDNGVRLGQQHCLVRYLEQEPELDPALTLAATVMRGLAADVEPTA
                     AAHRATDLLAELELDPDRLATGLSGGELRKVALARALVGRPDVLLLDEPTNHLDLPTI
                     IWLEATLARSRAARVIISHDRSFLAGVSDRTWWLDRGRLLVNNAGCGSFEAWRDQVLA
                     EEEKTLARLDKHLEAETHWLHRGVTARRRRNQGRMRKLGELRAQRAGFLRPEGRAKLE
                     SHLAKGGGSLVIEAIGITKTLGDRLIVDRFSTRVLKGDRVGVIGANGSGKTTLLNLLT
                     GTLAPDSGEVRLGTGLAIARFEQDRGSLDPQATPWSTLCPDGGDQVEAGGRPRHVVGY
                     LRDFLFSEPQARQPIAALSGGERNRLLLARNLARPSNLLVLDEPTNDLDLETLDLLED
                     LLADYRGTVLLVSHDRDFLDRVATSTIVLEGNGRAVEAAGGYSDALRQGCRIGGVERP
                     QPASSAAGSGRDPAMFERRREAGKVNARLARELDRLPDRIDAATSAVAALEERLGDPR
                     LHTEEPQKAQRLAEDLAAARTELAILEDRWLELETQRETGAL"
     gene            8086..9570
                     /locus_tag="P5558_07200"
     CDS             8086..9570
                     /locus_tag="P5558_07200"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRY24131.1"
                     /translation="MVEALVEAFERNGIREIAVLDEPDAAVAQVAQHYDAAVGRLASA
                     FAAYVGQGTLPGELDACYPFVGVRVAADAVNLDARIAYGALHDPGLYGTTLTRPSLFG
                     DYYRGQFAILLANHGAPLVVGVSRRPIPLPFVVDVAASGLDAERVHRLTHLFPMPDLA
                     ATDDSIANGTFHSQPGEPGALALFTAERVDYSLQRLSHYTGTSTQYFQRFVLLTNYQR
                     YVEHFVDYARRELAAGDAYDRFIEPGNVVTVRSGAPAPERNGTAPQHLPQMPAYHLTR
                     PDGRGVTLVNIGVGPSNARTITDHIAVLRPHCWLMLGHCAGLRRTQRLGDYVLAHGYV
                     REDHVLDQEVPPFVPVPPIAEVQVALQQALANVTGLADLDLKSRMRTGTVASTANRNW
                     ELRYEEFFQRLNQSRAIALDMESATIATNGLRFRVPYGTLLCVSDKPLHGEIKLKGMA
                     NRFYEERVNQHLEIGLEAIRLLRLQGPERLHSRKLRSFDEPAFR"
     gene            complement(9688..10860)
                     /locus_tag="P5558_07205"
     CDS             complement(9688..10860)
                     /locus_tag="P5558_07205"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRY24132.1"
                     /translation="MRLARFCTTCLLSAAVSMFASCGSWHMSSSLGAGELPSNVASEE
                     ASFSVERVTGGLEHPWGMAFLPDGRILVTERPGRLRLIEADGTLDPTPLEAVPTAFAR
                     GQGGLLDVALHPGFNANRLVYLSFSQLGGRGAVTAVARGTLEENGLAGTEVIFTSNGQ
                     TSHTRHFGSRLAFDTDGRLWVTHGDRGERDLAQDLKSHAGSVLRLTDDGGAPEDNPFV
                     GQRGAMPEIYSMGHRNPQGLAIHPATGAIWIHEHGPRGGDEINILEAGGNYGWPVVSQ
                     GREYASGRAVGASEAAGMTPAIYIWDPSIAPSGMAFYDGEAFPEWRGDLFVGALAYQL
                     LARLELDGDRVVHEERLLEDELGRIRDVAVGPNGFLYLLTDEADGGLFRLVPALQP"
     gene            complement(10908..11591)
                     /locus_tag="P5558_07210"
     CDS             complement(10908..11591)
                     /locus_tag="P5558_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY24133.1"
                     /translation="MADRAPVSLRQCKLFEELDEPVLESVAAVLRLQTYVAGSTIFAQ
                     YDTSHDVDFILNGRVRVTLYSRSGKEVTFRDLAAGDMFGELAALDREPRSAHVIALTE
                     VTLASMSGEDFHRLLRQHPDFAMLVLRKLSHLVRLLSERVYQFSTPVPTRVCAELLRR
                     AEEAPLAANVARIVPAPKHAEIANCVNTHREAVSRTMSDLMRQGILRRGSGELIVLQV
                     DRLRDLADH"
     gene            11890..13455
                     /locus_tag="P5558_07215"
     CDS             11890..13455
                     /locus_tag="P5558_07215"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HRY24134.1"
                     /translation="MSMNGAELLIGAARDLGLEVCFANPGTTEMPLVNALDAADVGAN
                     RGIRGVLGLHENVCTGAADGYARMAGKPALTLLHLGPGFANGLANLHNARRARSPVVN
                     LIGEHASWHLSADAPLHSEIERLTGWAGTWTRRSARAADLGRDLADALGAATSGRGHV
                     ASLILPHDLQLEEVAAATPWSLHARGMPAVDAERVRAAVRCLEAARRPAIMLGGSALF
                     GAGLEAAGRLAERTGAALIGEVGYARMAAGRGLPRVRRLPYFPELAAELLAGFDAVIV
                     AGTKLPVSFFGYHDKPARYLEGREDVVHLADGDEDAETALEALLAELPAGPAAAVLNV
                     AAPSDPGGEALDAAVIGAVLAAEQPEDAIVVVTAVSSAAPYGQFALDAAPHTQLALTG
                     GAIGEGLAMALGAAVAAPDQRVIAFEADGSGAYIMQALWSQARLGAKVTTVICANRRY
                     RILEMEQERAGIEAGSIARDLTSLDRPELDWVGLARAMGVPGEAVLDRTALALALRRG
                     LAEAGPYLIEARF"
     gene            13585..16530
                     /locus_tag="P5558_07220"
     CDS             13585..16530
                     /locus_tag="P5558_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HRY24135.1"
                     /translation="MEADLQSTEGSATNDDPLDAAPTSCPEPQSAPEAIVAIGASAGG
                     LEAIEQFFGEVEAPSRLAFVIVQHLSPDLKSLMRELLSRRTTLALPPVVDGMRPAANA
                     IYLIPPGKRMTIEGGVLLLADRDARDFAYHPIDEFFLSLAADQGAAAIGVVLSGSGSD
                     GSRGARAILRAGGRVLVQAPATAKFSSMPKAALEAGCTNDVMPPEAMPRALLEVEARP
                     RPEHGKLADNGLPTLLVDTLDATFGIDFSSYRAGTVQRRVRRRMMLRGFEQIQDYAEL
                     VADDGDEQEALYQDLLIGGTAFFRDAEAFEALERFVMPALAAELDAGREVRIWVPAVA
                     TGEEAYSIAILLLAQCREQAGEPPLRIFATDVHRRSLAVAAAGHYSEEAVRNLRPEWL
                     ERYFSRRGSGYQAIPALRRCIVFSPHDLLKDPPFTRLQLVSCRNLLIYLKKAAQEEVL
                     QNFSAALQADGHLLLGSSESPGTLDADFEAMAGALSLYRRRPGSTSLARPALVPSVLA
                     AGGQAGGRNPPVPSGGAARPERRDQRLTMAHELLLDRYVPPSLLVDAAGLVVHCFGDA
                     GAYLRQPRGRPSLALLDMIVEPLRGPLGSAMSRVRRTGHPITLRHVAAGDDHVLQRLV
                     VEMLPAPGDGRRCLLVSFDDRQGPVETGESEPAIEPDARAAERIRELERDLGFAEESL
                     RATVEELGSANQELLAANEELRAANRELQSAMNELHSVNDELQAVGTEHQRRIAALVQ
                     SAQDTESLLASAELAAVFVDADLRVRRFTPALGRFFNLIAQDIGRPLAHLTHGFGDAD
                     IVGRLRDVTATGDILSADLLAADGAVVRLRAVPHRGSGEQVTGVTVTFVDVTDSLRQA
                     EALARARIGVAVLLEALPQPAALANGTGRLTAANVPMEEVAAAAGLMPDLVGRELGWL
                     LGPFLDATALEELSRRLATGADWRHRMVTEGGGALSVELRSSDGPTVVLVLLEPPRGS
                     AVAPPATDC"
     gene            16561..17139
                     /locus_tag="P5558_07225"
     CDS             16561..17139
                     /locus_tag="P5558_07225"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRY24136.1"
                     /translation="MRRLIVGISGASGVILGERLLRALAPLEIETHLVLTRSAALTAT
                     YELETGVEALKALASVVHPIGDVGASIASGSFPTEGMIIVPCSMRTLAAIATGVTDNL
                     LTRAADVVLKERRRLVLMARETPLHLGHLRNMVAVTEMGAIVAPPVPGFYAKPASIEA
                     MVDHTVGRALDLFGIETEGVTRWRAGAAPSAD"
     gene            complement(17164..17249)
                     /locus_tag="P5558_07230"
     tRNA            complement(17164..17249)
                     /locus_tag="P5558_07230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17213..17215),aa:Leu,seq:gag)
     gene            17500..18231
                     /locus_tag="P5558_07235"
     CDS             17500..18231
                     /locus_tag="P5558_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRY24137.1"
                     /translation="MDERRAAVIAVGNLKGGTGKSTIAVNLACALAAAGEPTIVIDAD
                     PQLTATRWLRGRKVPASVFEAPLGDFAATPAWIAELQEIRRHNAFVIIDLPAVLGAAL
                     ASALLLADLMLIPTSPSAIDLSATTRTLQRVARAAAERRMGVLHSLVVPTRIDAGWFD
                     DGGLRRLKIRVPSPLAPPIRRSELFAQAFDAGNWIGGFAPRSPAHRDIRALMAAARKA
                     LKSPPPLPKPTAVRTAAVIAAGLTA"
     gene            complement(18253..19818)
                     /locus_tag="P5558_07240"
     CDS             complement(18253..19818)
                     /locus_tag="P5558_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRY24138.1"
                     /translation="MESDPALLIGKGETPQRLLLRLANRHGLITGATGTGKTITLQIL
                     AEAFSRAGVPVFCADVKGDLSGIAMAGEPNPKLQARATELGDADYVCAATPAVFWDLF
                     GEQGHPVRATISEMGPLLLARLMELNEVQEGVLTIAFRVADDEGLLLLDLKDLRSILV
                     FLADNADAVAKTYGNVSKSSIGAIQRRLLVLEEEGADRFFGEPALTLDDLMRTDDAGR
                     GIVNLLMADRLMRSPRLYGTFLLWLLSELFETLPELGDPEKPVLVFFFDEAHLLFDNS
                     PKALLEKVEQVVRLIRSKGVGVYFVTQNPIDVPPIVLGQLGNRIQHALRAFTPRDQKA
                     VKVAAETFRPNPAFVTEEVITALGVGEALVSTLMSKGEPSMVERTKILPPRSRMGPLT
                     SDERRELIDRSPLFGRYEDLVDRESASEMLQQRAAEQAREAAAAELDAALARQQELGE
                     ELGSSRRIGSKGRQSAPEPSSGGILADIFTGGGGRRQSPAEAMAKSMARSVGSQLGRT
                     LVRGILGSLMKGR"
     gene            19947..20903
                     /locus_tag="P5558_07245"
     CDS             19947..20903
                     /locus_tag="P5558_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY24139.1"
                     /translation="MDVKSMALRALRPKRPADRVYPFRGTAQVTETRLRLPDGRHVGV
                     ARAGPVCAEPLVYLHGILGSRLEAFVGGEPRHGVIGIDRPGYGLSDPLPRPSLAQYGR
                     DVEAVIDGLGIERCCLFGVSAGAPYTLAAALRLGRRVRELFLVAGVAHAGLVRTAGLP
                     MRLLVDFAEQPVLREKVVPNLRELMSRPLVANGWLRMTLTAERELLPSTAVMQFLARR
                     LARSMVAGNAHGPEGVQTDLRLLTTPWDFDHTALDCPVRVVHGLNDGVVAPNHGRWYA
                     RHLPAARLKLLPRYRHVSTILFTGPKIVAMARCHERRGQTDA"
     gene            complement(20912..21214)
                     /locus_tag="P5558_07250"
     CDS             complement(20912..21214)
                     /locus_tag="P5558_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24140.1"
                     /translation="MSIRHPVLPILLAMALAGTPGTPLLAQSSWEEDLTAEIQIMEDC
                     TVSFLSQIVERQIDGRSMIMAKVHCEDGRAFDATRDDSFAAFEFRACEPEDKPAAC"
     gene            21453..>21640
                     /locus_tag="P5558_07255"
     CDS             21453..>21640
                     /locus_tag="P5558_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24141.1"
                     /translation="MAGIQRTRRMIYVMAAFGLVLLATTGPARAAGGDGLREALAAVD
                     GLGNEAVAILRDAGLEPTA"
BASE COUNT         3184 a         7287 c         7752 g         3417 t
ORIGIN      
        1 caccacctag cgaccaaagc gaggcgcggc tagtctcgcg tgcaggggcg ggcacgggcc
       61 agccgaagcg aggttgccgc gtggccgcga gccaggtcct ttccggcatt gtcgaccggg
      121 tcgtcgtcca gaatccgacg accggcttcg cggtgctcag ggtgaaggtc ccgggccgga
      181 gcgagtcgat cactgtcgtc ggcgcggtgg cgaacagccg ccccggcctg gtcgtacggg
      241 ccgagggcga ctggcaggac gacccggctt gggggcacca gttcgcggcg acccgtatca
      301 ttcttctggc accggcaagc gtggatggga tcagggccta tctggcatcg ggtgccatca
      361 agggtgtcgg cgaggctttg gcgcaacgga tcgtcgagca tttcggggcg gacctgcgcc
      421 acgtgctcga cgaggcaccg gagcgcctcc gggaggtgcc cggcatcggc gcccggactt
      481 tgcagcggat cacggaggtc tggcgcaacg agggcgagaa ccgcgacatc ctgctgttcc
      541 tcgagagcca ggggattcgc ccggcgcggg cgcgccgcat cctggaggcc tatagcggcc
      601 gggcgatcga gcagctcctg gccgacccct acgcgctggc ccgcgacatc cggggcatcg
      661 gtttcgatac ggccgatctt ctggccgacc agctcgggat cactgccgat gcaccggtcc
      721 ggcggcgggc cgcgctgctc gaggtgctgc gcggcaatgc cgagcgcggt cacagcgcca
      781 tgcacctcga gcgagcgttg aacgaagcgg ccactctgtc gggccagtct ccgacggcca
      841 tcgccgaggt ggccgatggt ttgcgccacg agcggcgcat cgtcgaacgg accaccgagg
      901 ccggcacctt cctcctgctg cccgaactcg atgcggcgga ggcgacgatc gtccgccgac
      961 tggccattct ctcggccggg ccggtgccct gggaccgcac ggccctgggc gattgggagg
     1021 ccgtcgtgcg cgtccgcctc gccttggagc tctcaccgag ccaggccagg gcgctcgccg
     1081 tgatcctgac ctccagggtc gcgatcctca ccggcgggcc gggaaccggc aagacatcgc
     1141 tggtccgggc atgcgtggcc tgcctggaac gtcagcaggt gcgcctggcg ctggccgcgc
     1201 ccaccggtcg ggcggcccgg cgtcttttcg agagcaccgg gcacgaggcc acgaccattc
     1261 accgactgct cgaggccgat atccagcgcg gctttgcccg caacgccgag cggccgctcg
     1321 acctcgatct cctgatcctc gacgaggtct cgatggtcga cctcccgctg atggagggtg
     1381 tcgtcgccgc cttgccggac cgggcagcac tcctgctcgt cggcgaccgc gaccagttgc
     1441 cgccggttgg gccgggccag gtgctggcgg acctgatcga cagcgagcga tttcccgtgg
     1501 tggcgctcac cgaaatcttc cgtcaggccg aggcgagcgg catcgtgcgg ggtgcccatc
     1561 gggtcattcg aggagagctg ccacgcttcg gcacggacga ggccggcgac tgctttggca
     1621 ttcgcgtgcg tgatgccgcc gacgcgcgcg ccaagctgat cgagctggtc caccaccgca
     1681 tgccggagcg tttcgacctg gacccggtcg ccgatatcca ggttctggtg ccggtcaatc
     1741 gcgggccggt tggcacgcgt gacttgagca gcaccctgca gaaggtgctc aacccgtcgc
     1801 ccgagatggc gttcgagcgt ttcggggtgc gtttcggaat cggcgacaag gtcatgcaga
     1861 tcgagaacga tcacagccgc gaggtctata acggcgatct cgggatcatt cagaagatcg
     1921 acagcggcgc ccggctcatg gaggtccgct tcgacgatcg cctggtcgtg tacgggttcg
     1981 attcggtcga ccagctggtc tccgcctatg cgatcaccgt gcacaaggcg cagggctcgg
     2041 aatatcccgc cgtcgtgctc ttgctcttgc acgagcacgg ccgcatgttg cgccggcaat
     2101 tgctctacac ggccatgacg cgggcccgcc gcctgctggt cgtgctcgcc cagggcagcg
     2161 cgctcgagcg ggccgttgcc acccccgagc cggcacggct gtcgctgctc gggcatcgcc
     2221 tcgcgaccga atgggccacc ggcccggccg gtgaacggac tagccccatc caacggacga
     2281 acccatgacc gatgaagacc tcgatcgccg cctcgatgcc ggcgtgccga ttctccatgc
     2341 cgccggccgg ctggcgctcg atttcttcca gcgccgcggt gagctggtga tcgagcacaa
     2401 gggcctacag gacctcgtca gcatcgccga ccagacggtc gagcgggcga tccgtgacga
     2461 gctcggccgg ctctttcccg gcgacgccat catcggtgag gaggatggtg ccggggcggg
     2521 ctacgccgcg gcgcccggtg tctgggtgat cgatccgatc gacggcacca gcaacttcct
     2581 gcgcggcatg ccctactggt gctgcgtgat ggcctatgtg gtcgacggga cgaccgagat
     2641 cggcctgacc tacgatccca tccacgacga gatgttcgcc tgccggcggg gtgcggaggc
     2701 cacgcgcaac agacagccga tcgaggtctc gagccgcccg gtggcggagg cctgcgtcgg
     2761 gctcagcttc aacttcaagc aggatgcccg ggaatatgcc gagatggtcg agcgcctgaa
     2821 cgcgcggtcg ttcgaccacc gccgctcggg ctcgagcgcg ctccatctct gccacgtcgc
     2881 ggacggccgc ctcgacggcg ccatcaaccg gcactgcatg agctgggacg tgctcgccgg
     2941 cctggcgctg gtcgaggcgg ccggcggcgt cgccacccat tacaccaggg gggctagcct
     3001 gatcgagcca cgagccgtgc tcgccacgac cccggcgctc agggcggtgg tcgaggaagc
     3061 ggcgggtttt ccgctcgatt cctgaacccc ggagaggcga ctacctcgct cacccggaga
     3121 aggcctggag cccggtctgc gcccggccga gaatgagggc gtggatgtcg tgcgtgccct
     3181 cgtaggtgtt gaccgcctcg agattcatca cgtggcggat cacatggtac tcgtcggaga
     3241 tgccgttgcc gccgtgcatg tcgcgggccg ttcgggcgat gtccagcgcc ttgccgcaat
     3301 tgttgcgctt gatgagcgag atcatctccg gcgccatggc gccctcgtcc atgagccggc
     3361 cgacgcggag cgccgcctga aggcccaggg tgatctccgt ctgcatgtcg gcgagcttct
     3421 tctggatgag ctggttggcg gcgagcggcc ggccgaactg ctggcgctcc agggtgtagc
     3481 cgcgggccgc atgccagcag aactcggcgg cgcccatggt gccccaggcg atgccgtagc
     3541 gcgcccggtt gaggcagccg aagggtccct tgaggccctt gacgttgggc aggagattgg
     3601 cctccggcac gagcacgtcg tcgagctgga tcatcccggt gatcgaggcc ctgaggctga
     3661 atttgccttc gatcttggga gtggtgaagc cctgcatgtc gcgctcgacg atgaagcccc
     3721 ggatcacgcc gtcgtcggtc ttggcccaga cgatggcgag gtcggcgatc gggctgttgg
     3781 tgatccaggt cttcgccccg ctcagccgaa agccgcccgg cgccttgcgc gccttcgtgg
     3841 tcatgccacc cggatcggag ccatggtcgg gctcggtcag gccgaagcag ccgaccagct
     3901 cgcccttggc cagcttgggc aggaacttcc ggcgctgggc ttcgtcgcca taggcatgga
     3961 tgggatgcat gacgagcgac gactgcacgc tcatcgccga gcgatagccg gaatcgacac
     4021 gctccacctc acgggcgatc aggccgtagg cgacatagga agcgtccgcc ccgccatagg
     4081 cttccggcag cgtgctgccg agaaagccga gcgcgccgag ctcggtcatg atctcctggt
     4141 cgaacacctc gtgccggttg gcctcgagca cccgcggcat caggccgttc tgacaatagt
     4201 cggcggcggc gtcgcggatc agccgctcgc tttcgtcgag ctggtcgccg agccgaaagg
     4261 ggtccgccca atcgaacacg gggcgagctt cggtgggccg gccgctctca gtctttgggc
     4321 ccggatcggc catggctgtc atgctgcatc tccatcgacg ttcccgatgg ggactataga
     4381 cccggtgcgg ccgcttggcc tatccctctg ctggcgaccg ggtcaccgca agcaagtaca
     4441 tggccggggt gaccctgctg gcggcgttca ggccggaagg tctgttcgat cggctgcgct
     4501 gtttctcccg actacggggc ggggcacacc ccgagcagtg gcggcgcgat cctgagatgt
     4561 gttcagacac gtgcgctgga gccgcttgaa gggcgtcgtg accgttcacc agccaaggac
     4621 cgggtcgacc gaactcgtca gccgttccag gtgcggacct tgccggtgtc gatatggacg
     4681 aactgcgact tcgggtagta gcctacgcca ccgcgctcga gctgccgggc aacctccctg
     4741 acgtcgcgca gccgggcccc ctcgaggcgc aggtcgatgg cccgtccgaa catgtgcagg
     4801 cttcgtttgg ccgcacctcc gcccgcccgg cgcagcatgg cgttggtttg cggcgagcga
     4861 tagcccgaga tgatctcgaa gcggggcgcc tggccgaggc ggtcggcgat ggcggccatg
     4921 atatcgtaga gccgtggatc gatcgggagg acttcctcgc gccgccagtc acgcaggaag
     4981 tagttcaggc tcgtgagcgc gcgccggtcg aactcctggc cctgccgata gacgacgtcg
     5041 agatcttcct tggtgtgcag gttgtgcagg agcagccgac gcggctgggt cgctgcctcc
     5101 agcggcgagg cggcaaggcc gacggcggcg gccgcaccgg tgatgaacca gcgacgggta
     5161 gatttctgca gactgtgcgt cggtgccatc gacgtcgatc tcctccggtt ccaggccggt
     5221 agggtgatag cgtgtccatg catgatggaa aaccgttaat cttctgtggt cgttccgcga
     5281 attccttcgt ccggatgact tgtgctcgcg gggctcggac gatagcagag ccagtaggtg
     5341 aggccgacga agcctccacc gccgatgatg ttgccgaccg tcaccggcac gagattggcg
     5401 agcagcccgt cgatcccgac gcccggcgcg ccattcatcc aggcgagcgg gatgaagtac
     5461 atgttcgcca ccgaatgctc gaagccgagc gccacgaagg cggcgatcgg caggagaatg
     5521 gcgaggatct tgccggtgac gtcgcgggcg gcgaaggtga gccagaccgc gaggcagacc
     5581 agggcattgc agagcaggcc gcgaaacagc gcctcgtgcc agacgaggcc ggtcttcgcc
     5641 agggcgatcg cgaaggccgt ctcgcgcacg gcgccgtcgc cgagcgcgag cgtgcccgag
     5701 agggtcagca gcacggcggt gccgacggca ccgatgaagt tcgcgacata gaccagcgtc
     5761 cagttgcggg cgagatcgag gagcgtgatc cggcgatcga cccaagccat gacgatgagg
     5821 ttgttgccgg tgaagagctc ggcaccggca atcacgacga ggatgagccc gagtgagaag
     5881 accagcccgc cgagcaggcg ggccgggccg aaaccgagcc cgtcaccggt catcaccagg
     5941 gtgtagaaca tcgagcccat ggcgatgaac gcaccggcga gcagaccgag cgtcagggtc
     6001 tgcagggtgc cgagacgggc cttcgccacg ccggaccgct cgatgagccg ggcgatctcg
     6061 gccggcttgt aggcatccag atcaaggctc ctggccagtt ccatggcgat gctcccgtcc
     6121 gggttccgac gcttcagaga gcgccggtct cgcgctgggt ctcgagctcg agccagcgat
     6181 cctcgaggat ggcgagctcg gtcctggccg ccgccagatc ctcggcgagg cgctgcgcct
     6241 tttgcggctc ctcggtgtgc aggcgagggt cgccgagccg ctcctcgagc gcggccacgg
     6301 cgctggtcgc ggcatcgatc cggtcgggca ggcgatcgag ctcgcgagcc agtcgcgcgt
     6361 tcaccttgcc ggcctcccgg cgtcgctcga acatggcggg atcgcggccg ctgccggccg
     6421 ccgaagacgc cggctgcggc ctttcgaccc cgccgatccg gcaaccctga cgaagagcat
     6481 cgctgtagcc gccggcagcc tcgaccgcgc gtccgttgcc ctcgagcacg atggtgctgg
     6541 tcgccacccg gtcgagaaaa tcgcggtcgt ggctgaccag gagcaccgtg ccgcgatagt
     6601 cggcgaggag gtcctcgagc agatccagcg tttccaggtc gaggtcattg gtcggctcgt
     6661 cgaggaccag gagattgctc ggccgggcca ggttgcgggc aaggaggagg cgattgcgct
     6721 caccgcccga gagtgcggcg atcggctgcc gcgcctgcgg ttccgagaag aggaagtcgc
     6781 gcagatagcc gaccacatga cggggcctgc caccggcttc gacctggtcg ccgccatccg
     6841 ggcaaagcgt cgaccagggt gtcgcttgcg gatcgaggct gccccgatcc tgctcgaagc
     6901 gggcgatggc gaggcccgtg cccagtcgca cctcaccgct gtcgggtgcg agcgtgccgg
     6961 tcaggaggtt gagcagggtc gtcttgcccg agccattggc gccgatgacg ccgacccggt
     7021 cgcccttgag gacgcgggtc gagaagcgat caacgatcag ccggtcaccg agggtcttgg
     7081 tgatgcctat tgcctcgatc acgaggctgc cgccgccctt ggcgaggtga gattcgagct
     7141 tggcgcggcc ctccggccgg aggaatccgg cgcgttgcgc gcgcagctcg ccgagcttgc
     7201 gcatgcgacc ctggttgcgc cgcctccgtg cggtgacacc gcgatgcagc cagtgcgtct
     7261 cggcttcgag atgcttgtcg aggcgcgcga gggtcttctc ctcctcggca agcacttggt
     7321 cgcgccaggc ctcgaagctg ccgcacccgg cattgttgac gagcagccgg ccgcggtcga
     7381 gccaccaggt ccggtcgctg accccggcga ggaacgaccg gtcgtggctg atgatgacgc
     7441 gcgcggcgcg gctccgggcg agagtcgcct cgagccagat aatggtcggc agatcgagat
     7501 ggttggtcgg ctcgtcgagc aggaggacgt ccggccgacc aaccagtgcc ctggcgagcg
     7561 ccaccttgcg cagctcgcca cccgaaagcc cggtcgccaa ccggtcgggg tcgagctcga
     7621 gctcggcaag cagatcggtc gcccgatgag cagctgccgt cggctcgacg tcggcggcga
     7681 gaccgcgcat gacggtggcc gccagagtga gggccgggtc gagctcgggc tcctgctcga
     7741 gatagcggac gaggcagtgc tgctggccga gccggacgcc gttgtcgagc tcgatctgac
     7801 ccgccagcac cttgagcagg gtcgacttgc cgctgccgtt gcggccgacg aggccgatcg
     7861 cggcgccggc cgcaatgtcc agctccaggt cgtcgaaaag ccgcttgccg cccaggctga
     7921 gcgacgcgcg gcgcaaggaa agaagtgtgg ctgcggccat tgagggcggc tctcgaaccg
     7981 gtcggggtcg gctggtggtt gtcgaaaaaa gcggctagag cgacgactgg tcggcagccg
     8041 gcgccaccct cgggtggcgg tgggttctta gcggagcgag aaggtatggt cgaggcgctg
     8101 gtcgaagcct tcgagcgcaa tggcattcgc gagatcgccg tcctcgacga accggacgcc
     8161 gccgtggcgc aggtggccca acactacgat gccgccgtcg ggcgtctggc ttcggccttc
     8221 gccgcctatg tggggcaagg cacgctgccc ggcgagctgg atgcctgcta tccgttcgtc
     8281 ggcgttcgcg tggcggcaga tgccgtcaac ctcgacgcgc ggatcgccta tggagcgctg
     8341 catgatcccg gcctctacgg cacgacgctg acgcgaccgt ccctcttcgg cgactactat
     8401 cgcggccagt tcgcgatcct gctcgccaat cacggcgcgc cgctggtggt cggtgtcagc
     8461 cgccggccga ttcccttgcc cttcgtcgtc gacgtggcgg cgagcgggct cgacgccgag
     8521 cgcgtgcacc ggctcaccca cctcttcccg atgcctgatc tcgccgcaac cgatgacagc
     8581 atcgccaacg ggacgttcca ctcgcagccg ggggagcccg gcgccctcgc actgttcacg
     8641 gccgagcgcg tcgactacag cctgcagcgg ctgagccatt acaccggcac gtccacccag
     8701 tatttccagc gcttcgtcct gctgacgaac taccagcgct atgtcgagca cttcgtcgac
     8761 tatgccaggc gagagctggc ggccggcgat gcgtatgatc ggttcatcga gcccggcaat
     8821 gttgtcacag tcaggtccgg ggcacccgca cccgagcgaa acgggacggc gccacagcat
     8881 ctgccgcaga tgccggccta ccacctgacc cggccggatg gtcgcggggt cacgctcgtg
     8941 aatatcggcg tcggcccctc caacgcacgg accatcaccg atcacatcgc cgtgctgcgc
     9001 ccgcattgct ggctaatgct cggccattgc gccggcctac ggcgcaccca gcggctcggc
     9061 gactatgtgc tggcccacgg ctatgtccgt gaggatcacg tgctcgacca ggaggtgccg
     9121 ccgttcgtgc cggtgccgcc gatcgccgag gtgcaggtgg cgctgcagca ggcgctggcc
     9181 aatgtcaccg ggctcgccga tctcgacctg aaaagccgga tgcgcaccgg caccgtggca
     9241 tcgaccgcca atcggaactg ggagctgcgc tacgaggagt tcttccagcg gctgaaccag
     9301 tcgcgcgcca tcgccctcga catggagagt gcgacgatcg ccaccaacgg cctgcggttt
     9361 cgggtacctt atggcactct gctctgcgtc tccgacaaac cgctgcacgg cgagatcaag
     9421 ctgaagggca tggccaaccg tttttacgag gagcgcgtca atcagcatct cgagatcggc
     9481 ctcgaagcca tccggctgct gcgcctgcag ggtcccgagc gcctccattc ccgcaagttg
     9541 cgcagcttcg acgaaccggc gttccgctag agtatctttg ttgatcaaga agtgcgttcg
     9601 agcgtccagt ttctgttgct gcggagcagg cgcgaagaac ctgccgattg aagatacttg
     9661 aagggattcg gcattcggaa attcaatcta gggctgtaag gctggcacca gccggaagag
     9721 accgccatcg gcctcgtcgg tgagcagata gaggaaaccg tttgggccga ccgccacgtc
     9781 gcggatgcgg ccgagctcgt cctcgagcag gcgctcctcg tgcacgaccc ggtcgccgtc
     9841 cagctcgagc cgggcgagga gctggtaggc gagagcgccg acgaagagat ctccccgcca
     9901 ttcggggaag gcctcgccgt cgtagaaggc catgccggaa ggggcgatcg agggatccca
     9961 gatgtagatc gccggggtca tgccggcggc ctcgctggcg cccaccgccc ggccggaggc
    10021 gtattcccga ccctggctga ccaccggcca gccgtaattg ccgcccgcct cgaggatgtt
    10081 gatctcgtcg ccgccgcgcg gcccgtgctc gtggatccaa atcgcaccgg tggccggatg
    10141 gatggcgagg ccctgcgggt tgcggtggcc catggagtag atctcgggca tggcgcctct
    10201 ctggccgaca aatgggttgt cttcgggggc gccgccgtca tccgtcaggc ggagcacgct
    10261 gcccgcatgg ctctttaggt cctgcgcgag gtcgcgctcg ccacggtcgc cgtgggtcac
    10321 ccagaggcgg ccgtccgtat cgaaggcgag gcgcgagccg aaatgccggg tgtggctggt
    10381 ctggccgttg gaggtgaaga tcacctcggt gccggcgagc ccgttctcct cgagcgtacc
    10441 tctcgccacc gctgtgaccg caccccgtcc gccgagctgg ctgaagctca aatagacgag
    10501 ccggttggcg ttgaagccgg ggtgcagggc cacgtcgagc aacccgccct ggccgcgcgc
    10561 gaaagcggtc gggacggctt cgagcggcgt cgggtcgagc gtgccgtcgg cttcgatcag
    10621 ccggaggcgg cccgggcgtt ccgtcaccag gatccgcccg tccggcagga aggccatgcc
    10681 ccagggatgc tcgagtcccc ccgtgacccg ctcgaccgag aagctggcct cctcgctggc
    10741 gacgttgctc ggcagctcgc cggccccgag gctgctgctc atgtgccagc tgccgcaaga
    10801 ggcgaacatg ctcacggccg cggagagcag gcaggtggtg cagaagcggg cgagccgcat
    10861 gtcgatctcc cgtcgttccg gaacccgggg aggagatagg gtactggtca gtgatcggcg
    10921 aggtcgcgca gccgatccac ctgcaggacg atcagctcgc cgctgccgcg gcggaggatg
    10981 ccctggcgca tcaggtcgct catggtccgc gacaccgcct cacggtgggt gttgacgcag
    11041 ttcgcgatct cggcgtgctt gggtgccggg acgatgcgcg ccacgttggc ggcaagcggc
    11101 gcctcctcgg cgcggcgcag gagctcggcg cagacgcgcg tcggcacggg cgtgctgaac
    11161 tggtagacgc gctcgctcag cagccggacg agatggctga gcttgcgcag gacgagcatg
    11221 gcgaaatcgg gatgctggcg cagcaggcga tggaagtcct cgccgctcat cgaggccaac
    11281 gtcacttcgg tcagggcgat gacgtgggcc gagcggggct cgcggtcgag cgcggcgagc
    11341 tcaccgaaca tgtcgccggc ggcgagatcg cggaaagtca cctccttgcc cgagcgcgag
    11401 tagagcgtga cgcgcacgcg gccgttgagg atgaagtcga cgtcgtggct ggtgtcgtac
    11461 tgggcgaaga tcgtgctgcc ggcaacgtag gtctgcaggc gcagaactgc ggcgacgctt
    11521 tccaggaccg gctcgtcgag ctcctcgaag agcttgcatt ggcgcaggct gactggcgcg
    11581 cggtccgcca ttgttctttt ccgggctgtt gctccgcgtc gaagcgggcg cccaagtgag
    11641 cgcaacaggg cgcggctggc aagcctgcct gttgccgggg cgcactaggc gcgaaaaaat
    11701 tgctgctcag tgacgtaggc cacagttttt cgctcatcaa acgataatgt tctggcgtgc
    11761 ctccctgaac caaccttagg cgcggccgat gcgcagatcg cgggtcggcc gttttttttg
    11821 tcttgtcagc gacccgcccg acgggtcagg actgggcggg tcggaaggaa tccgcaagcg
    11881 ggatggggaa tgtccatgaa cggggccgag ctcctgatcg gtgctgcacg ggatctcgga
    11941 ctcgaggttt gcttcgccaa tccgggcacc accgagatgc cactcgtcaa cgcgctcgac
    12001 gccgccgatg tgggcgccaa tcgcggcatc aggggcgttc tcggcctgca cgagaacgtc
    12061 tgcaccgggg ccgctgacgg atatgcccgg atggccggaa agccggcgct gaccctgctg
    12121 catctgggtc cgggcttcgc caacggcctc gccaacctgc acaatgcgcg tcgcgcccgg
    12181 tcccccgtcg tcaacctgat cggcgagcat gcgagctggc acctctccgc cgacgcgccc
    12241 ctgcacagcg agatcgagcg cctcaccggc tgggccggca cgtggacgcg acgctcggca
    12301 cgggccgccg atctcggccg cgacctcgcc gatgcgctag gtgccgccac cagcgggcgc
    12361 ggccatgtcg ccagcctcat cctgccacac gacctgcagc tcgaggaggt ggcggcggcg
    12421 acgccctggt ccctgcatgc tcgcggcatg cccgcggtcg atgcggagcg ggtgcgggcg
    12481 gccgtgcggt gcctcgaggc ggcgcggcgg ccggctatca tgctgggtgg ttcggcactc
    12541 ttcggcgccg ggctcgaagc ggcggggcgg ctggcggagc ggaccggggc ggcgctgatc
    12601 ggcgaggtcg gctacgcccg gatggcggcg ggccggggcc tgccgcgggt gcgacgcctg
    12661 ccctatttcc cggagctggc agccgagctc ttggcgggct tcgacgcggt gatcgtcgcc
    12721 ggcaccaagc tgccggtctc gttcttcggc tatcacgaca agccggcgcg ctatctcgag
    12781 gggcgggaag acgtcgtgca tctggcagat ggcgacgagg atgccgaaac ggctctcgag
    12841 gcactcctcg ccgagctccc ggccgggcca gccgccgcag tcctgaacgt ggcagcgccg
    12901 tcggatcccg gcggcgaggc gctcgatgcg gcggtgatcg gtgccgtgct cgcggccgag
    12961 cagcccgagg atgcgatcgt cgtcgtgacg gcagtgagca gtgctgcacc ctacggccag
    13021 ttcgcgctgg atgcggcgcc gcacacgcag ctcgcgctga ccggaggggc gatcggcgag
    13081 ggtctcgcca tggcgctcgg ggcggccgtg gcggcaccgg accagcgggt gatcgccttc
    13141 gaggcggacg gcagcggtgc ctacatcatg caggcgctct ggtcgcaggc gcggctgggt
    13201 gccaaggtca ccaccgtcat ctgcgccaac cggcgctacc gcatcctcga gatggagcag
    13261 gaacgggccg gcatcgaggc cgggtcgatc gcccgcgacc tgaccagcct cgatcggccg
    13321 gagctcgact gggtcggcct ggcgcgggcc atgggggtgc cgggcgaagc cgtgctcgac
    13381 cggactgcgc tggcccttgc cctgcgccgg ggcctggcgg aggccggtcc ctatctgatc
    13441 gaggcgcgct tctgacggcg acggacgtgt aaaccataac gcaagttcca gaagcgcgta
    13501 ttcacgcttg gagttctcaa cgcgcgtcga atgctctagc tttgcagcag cggtgtgacg
    13561 aacccggggg tgggtgtgtc gacgatggaa gcggacctgc agtcgaccga gggctcggcg
    13621 accaacgacg acccgctcga cgccgccccg accagttgtc cggagccgca gtcggcgccc
    13681 gaagcgatcg tggcgatcgg cgcttcggcc ggcgggctcg aggcgatcga gcagttcttc
    13741 ggtgaggtgg aggcgccgag tcggctggcc ttcgtcatcg tccagcatct gtcgccggac
    13801 ctcaagagcc tgatgcgcga gctcctgagc cggcgcacga cgctggccct gccgcccgtg
    13861 gtcgacggga tgcgcccggc ggcgaatgcc atctacctga tccccccggg caagaggatg
    13921 accatcgagg gcggcgtcct gctgctcgcc gatcgggacg cgcgggactt cgcctaccat
    13981 ccgatcgacg agttcttcct ctcgctcgcc gccgaccagg gggcggcggc catcggcgtg
    14041 gtgctctcgg gcagcggcag cgacggcagc cgcggtgcgc gcgccatcct tcgcgccggt
    14101 ggccgggtgc tggtgcaggc gccggccacc gccaagttct cgagcatgcc gaaggcggcg
    14161 ctggaggcgg gctgcaccaa cgacgtgatg ccaccggagg cgatgccccg ggccctgctc
    14221 gaggtcgagg ctcggccgcg gcccgagcat ggtaagcttg ccgacaatgg gttgccgacg
    14281 ctcctggtcg acacgctcga tgcgaccttc ggcatcgatt tctcgagcta ccgtgccggg
    14341 accgtgcagc gccgggtgcg gcggcggatg atgctgcgcg gcttcgaaca gatccaggac
    14401 tatgccgagc tggtcgccga cgacggcgac gagcaggagg cgctctatca ggacctcctg
    14461 atcgggggga cggcgttctt tcgcgacgcc gaggctttcg aggccctgga gcggttcgtc
    14521 atgccggcgc tcgcggccga gctcgacgcg gggcgcgagg tgcggatctg ggtcccggcg
    14581 gtggcgactg gcgaggaggc ctactcgatc gccattctgc tcctggcgca atgccgggag
    14641 caggccggcg agccgccgct gcgcatcttc gcgaccgacg tgcatcgccg ctcgctggcg
    14701 gtggcggcgg cgggacacta cagcgaggag gcagtgcgca acctccgccc ggagtggctg
    14761 gagcgctact tcagccggcg cggcagcggc tatcaggcga tccccgccct ccgccgctgc
    14821 atcgtcttca gcccgcacga cctcctgaag gacccgccgt tcacccggct gcagctcgtc
    14881 agttgccgca acctgctgat ctatctcaag aaggcggcgc aggaggaggt cctgcagaac
    14941 ttttccgcag ctctgcaggc ggatggtcac ctgctgctcg gctcgagcga gagcccgggc
    15001 acgctcgacg ccgacttcga ggcgatggcg ggtgcccttt cgctttatcg ccggcggccc
    15061 ggcagcacct cgctcgcccg gccggcgctg gtgccgtcgg tgctggcagc cggcgggcag
    15121 gcgggggggc ggaacccgcc agtgccatcg ggcggcgcgg cgcggcccga gcgccgcgac
    15181 cagcggctga ccatggcgca cgagcttctg ctcgatcgct acgtgccgcc gagcctgctg
    15241 gtcgacgcgg ctggcctcgt ggtgcactgc ttcggcgatg ccggcgccta cctgcgccag
    15301 ccgcgcggcc ggccgagcct cgccttgctc gacatgatcg tcgagccgct gcgcgggccg
    15361 ctcggctcgg ccatgagccg ggtgcggcgc accggccacc cgatcaccct caggcacgtc
    15421 gctgccggcg acgatcacgt gctgcagcgg ctggtggtcg agatgctgcc ggcaccgggc
    15481 gatggccggc gatgcctgct ggtcagcttc gacgaccggc aggggccggt cgagacgggg
    15541 gagagcgagc cggcgatcga gcccgatgcg cgcgccgccg aacggatccg ggagctcgag
    15601 cgcgatctgg gcttcgcgga ggagagcctg cgggcgacgg tcgaggagct cggcagcgcc
    15661 aaccaggagc tgctcgcggc caacgaggaa ctgcgcgccg ccaaccggga attgcagtcg
    15721 gccatgaacg agctgcactc ggtcaacgac gagctgcagg ccgtcggcac cgagcatcag
    15781 cgccggatcg cggcgctcgt gcagagcgcc caggacacgg agagcctgct cgcctccgcc
    15841 gagctggcgg cggttttcgt cgacgccgac cttcgcgtgc gtcggttcac gcctgcactc
    15901 ggccgcttct tcaacctgat tgcccaggat atcggtcggc cgctcgccca tctgacgcac
    15961 ggcttcggcg acgcggacat cgtcggccgg ctgcgtgacg tgacggcgac cggcgacatc
    16021 ctgagcgccg acctcctggc cgcggatggc gccgttgtcc ggctgcgagc ggtgccgcat
    16081 cggggcagcg gcgagcaggt caccggggtg acggtgacct tcgtcgacgt gaccgatagc
    16141 ctgcgtcagg ccgaagcgct ggcccgggcg cggatcggag tcgcggtgct gctcgaggcc
    16201 ctgccgcagc cggcggctct cgccaatggc acaggccggc tgacggcggc caacgtcccg
    16261 atggaggagg tggccgcggc ggccgggctg atgccagatc tggtcggccg cgagctcggc
    16321 tggctcctcg ggccgttcct cgacgccacc gccctcgagg aactcagccg gcgactggcc
    16381 acgggcgcgg actggcggca tcggatggtg acggaggggg gtggggcgct gtcggtcgag
    16441 ctgcgcagct cggacgggcc gaccgtggtg ctcgtgcttc tggagccgcc gcggggcagc
    16501 gccgtcgcgc cgcctgcgac ggactgctag cctgcaattc ggcgaatggg cgaggggatc
    16561 atgcggcggc tgattgtcgg catttcgggg gcttcggggg tgatcctggg cgaacggctg
    16621 ttgcgcgccc tggccccgct cgagatcgag acgcatctgg tgctgacccg ctcggcggcg
    16681 ctgaccgcaa cctatgagct tgagaccggc gtcgaggcgc tcaaggcgct ggcgtccgtc
    16741 gtgcatccga ttggcgatgt cggggcctcg atcgcctcgg gctcgttccc gaccgagggg
    16801 atgatcatcg ttccctgctc gatgcgcacg ctcgccgcca tcgccacggg cgtcacggac
    16861 aacctcctga cccgggcggc cgacgtggtg ctcaaggagc gacgccggct ggtgctgatg
    16921 gcgcgcgaga cgccgctgca tctcggccat ctgcgcaaca tggtggcggt caccgagatg
    16981 ggggccatcg tcgccccgcc cgtgccgggt ttctacgcga aaccggccag catcgaggcc
    17041 atggtcgacc acacggtcgg ccgcgctctc gatctcttcg gcatcgaaac agagggcgtc
    17101 acgcgctggc gcgccggggc agcgccctcg gccgactgag ccggcatttc atgcaagggg
    17161 aggtggtgcg gtcgagagga ctcgaacctc cacgggctag gcccacagcg ccctcaacgc
    17221 tgcgcgtcta ccagttccgc cacgaccgca acgggaccgg gttctaacga aaagcgtgag
    17281 aagcgcaagg ggttgtgacc acccgcgcgc catccagcgt ggcagcccct ggttaacgtt
    17341 ccgttgacgc tgatgcatag gccggtgcgc aggcgggcct gccggggttg agccggatgg
    17401 ccatgatccg gatgctctgg ccgatgctcg cccgaggacg ggtgaggcgg catggaccgt
    17461 gccgacggcg ggtgccgtgc ggggcaggag gcctggacga tggacgagcg acgtgccgcg
    17521 gtgatcgcgg tcggcaatct caagggcggc accggcaaga gcacgatcgc cgtcaatctg
    17581 gcctgcgcgc tggcggcggc cggcgagccg acgatcgtga tcgacgccga tccgcagctc
    17641 accgcgaccc gctggctgcg tggccgcaag gtgccggcca gcgtgttcga ggcgccgctc
    17701 ggcgatttcg ccgcgactcc ggcctggatc gccgagctgc aggagatccg gcggcacaat
    17761 gccttcgtga tcatcgacct gccggccgtg ctcggcgctg ccctggcctc ggccctgctg
    17821 ctcgccgatc tcatgctgat cccgacgagc ccctccgcca tcgatctctc ggccaccacc
    17881 cgcaccctgc agcgggtcgc ccgcgccgca gccgagcggc gcatgggtgt cctgcacagc
    17941 ctggtcgtgc cgacccgcat cgatgccgga tggttcgacg atggcgggct ccggcgcctc
    18001 aagatccgcg ttccctcgcc gctggcgccg ccgatccgcc gctcggagct gttcgcccag
    18061 gccttcgatg cgggcaactg gatcggcggg ttcgcgccgc gctcgccggc gcatcgcgac
    18121 atccgggcgc tgatggcggc agcgcgcaag gcgctcaagt caccaccgcc gctgccgaag
    18181 ccgactgctg tccgaaccgc cgcggtgatc gccgccgggc tcaccgcctg atcgccggag
    18241 ggctcgggtc gctcagcgac ccttcatcag gctgcccagg atgccgcgca ccagcgtccg
    18301 gccgagctgg ctgccgaccg agcgggccat cgacttcgcc attgcctcgg ccgggctctg
    18361 ccgccgcccg ccgccgcccg tgaagatgtc ggcgagaatg ccgccgctcg acggctcggg
    18421 cgccgattgc cggcccttgc tgccgatccg gcgcgaggag ccgagctcct cccctagctc
    18481 ctgctggcgg gcgagtgccg cgtcgagctc ggcggcggcc gcctcgcgcg cctgctccgc
    18541 ggcccgctgc tgcagcatct cggatgcgga ctcgcggtcc acgaggtcct cgtagcggcc
    18601 gaagagcggg ctccggtcga tcagctcccg ccgctcgtcg ctggtcagcg ggcccatgcg
    18661 cgagcgcggc ggcaggatct tcgtccgctc gaccatcgag ggctcgccct tgctcatcag
    18721 ggtcgagacc agggcctcgc cgacgccgag cgcggtgatg acctcctcgg ttacgaaggc
    18781 cgggttgggg cggaaggtct cggccgccac cttgaccgcc ttctggtccc ggggcgtgaa
    18841 ggcgcggagc gcgtgctgga tgcggttgcc gagctgaccg aggacgatcg gcggcacgtc
    18901 gatcgggttc tgggtgacga agtagacgcc gacccccttc gagcggatca ggcggaccac
    18961 ctgctcgacc ttctcgagca gggccttcgg cgaattgtcg aagagcaggt gggcttcgtc
    19021 gaaaaagaag acgagcaccg gcttctcggg atcgcccagc tccggcagcg tctcgaagag
    19081 ctcggagagc agccagagga ggaacgtgcc gtagagccgg ggcgagcgca tcagccggtc
    19141 ggccatcagc aggttgacga tgccccggcc cgcatcgtcg gtgcgcatga ggtcgtcgag
    19201 cgtcagggcc ggctcgccga agaacctgtc cgcaccttcc tcctcgagca ccaggaggcg
    19261 gcgctggatg gcgccgatcg aggatttgct gacattgcca taggtcttgg ccacggcatc
    19321 ggcgttgtcg gcgaggaaga ccaggatgct gcgcaggtcc ttgaggtcga gcagcagcag
    19381 gccctcgtcg tcggcgaccc gaaaggcgat ggtcagcaca ccttcttgga cctcgttcag
    19441 ctccatcagc cgggcgagaa gcagcggacc catctcgctg atggtggcgc gcaccgggtg
    19501 gccctgctcg ccgaagaggt cccagaagac ggcgggggtg gcggcgcaga catagtccgc
    19561 atcgccgagc tcggtggcgc gcgcctggag cttcgggttc ggctcgccgg ccatggcgat
    19621 gcctgagagg tcgcccttca cgtcggcgca gaagaccggc acgccggcgc ggctgaaggc
    19681 ctcggccagg atctgcaggg tgatggtctt gccggtgccg gtggcgcccg tgatcagccc
    19741 gtggcgattg gcaaggcgga gcagcaggcg ctgcggtgtc tcgcccttgc cgatcagcag
    19801 cgccggatcg ctttccatgg gtcgagcctc cccgcctctt gccattttcg caggcatctt
    19861 ctttctagcc tccggccgac ctgtgacaag gggcggccgg cgaggccctg gcggcggcgc
    19921 catggctgcg gcgtggggag tggcggttgg acgtcaagag catggcgctc agggctctgc
    19981 ggccgaagcg cccggccgat cgggtctacc cgttccgggg cacggcacag gtcaccgaga
    20041 cgcggttgcg gctgccggac gggcgtcatg tgggggtggc ccgggccggc ccggtgtgcg
    20101 ccgagcccct ggtctatctg cacggcattc tcggcagccg gctcgaggcc ttcgtcgggg
    20161 gggagccgcg gcacggcgtc atcggcatcg accggccggg ctacgggctg agcgatcccc
    20221 tgcccagacc atcgctggcg cagtacggcc gcgacgtcga ggcggtcatc gacggcctcg
    20281 gcatcgagcg ctgctgcctc ttcggcgtct ccgccggcgc gccctacacg ctggcggcgg
    20341 ccctgcggct cggccggcgc gtgcgcgagc tcttcctggt ggccggtgtg gcgcatgccg
    20401 gcctcgtccg cacggccggg ctgccgatgc ggctcctcgt cgatttcgcc gagcagccgg
    20461 tgctgcgcga gaaggtggtg ccgaacctgc gcgagctcat gagccggccg ctggtggcca
    20521 atggttggct gcgcatgacg ctgaccgccg agcgcgagct cctgccgtcg acggcagtga
    20581 tgcagttcct cgcccggcgc ctcgcgcggt cgatggtggc cggcaatgcc cacgggccgg
    20641 agggcgtgca gaccgacctg cgcctgctga cgacaccctg ggacttcgac cacacggcgc
    20701 tcgactgccc ggtgcgcgtc gtccacgggc tcaatgacgg cgtggtggcg cccaatcacg
    20761 ggcgctggta cgcccgccat ctgcccgccg ctcgcctcaa gctgctgccg cgctaccggc
    20821 acgtctcgac catcctcttc accggcccca agatcgtcgc catggcgcgc tgccacgagc
    20881 ggcggggcca gacggacgcc tgaaggcggg atcagcaggc ggctggcttg tcctcgggct
    20941 cgcaggcgcg gaactcgaag gcggcgaagc tgtcgtcgcg tgtcgcatcg aacgcgcggc
    21001 cgtcctcgca atgcaccttg gccatgatca tcgaccgccc gtcgatctgg cgctcgacga
    21061 tctggctgag gaagctcacc gtgcagtcct ccatgatctg gatctcggcg gtcagatcct
    21121 cctcccagct cgactgggcc aggagcggcg tgccgggcgt gccggcgagc gccatggcga
    21181 ggaggatcgg caagacgggg tgacggatcg acatggccgc ggctcctgct gtttgggctt
    21241 tctattgaac cattatcatt cggtgcggac ggcgtcaccg ttgccgggat tgtgatcgtg
    21301 cgtgcgggtc cttggtcgag aacggcgcca cggtatgccc gcgagtgtga catcggccac
    21361 agtccccggc cgccttaaga ccctattcag caggtcggtg cagagatgcc gctgcttcaa
    21421 tcgggtgcgc gtacagcgcc aggaggaaac ccatggctgg gatccagaga acccggcgga
    21481 tgatctatgt gatggccgcc ttcgggctcg tgcttctggc taccaccggc ccggcgcggg
    21541 ccgccggcgg cgacgggctg cgcgaggcgc tggctgcggt cgacgggctc ggcaacgagg
    21601 cggtcgccat cctgcgcgat gccgggctgg agccgacggc
//
LOCUS       DAOTFI010000053        21577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2908_length_21577_cov_7.519933, whole genome shotgun sequence.
ACCESSION   DAOTFI010000053 DAOTFI010000000
VERSION     DAOTFI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21577
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_21577_cov_7.519933"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="P5558_07260"
     CDS             complement(<1..622)
                     /locus_tag="P5558_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY24142.1"
                     /translation="MKRDPGRLAGPELDVLVIGGGIYGACIARDAAQRGLRTALVERG
                     DFGGLTSHNSFKLIHGGLRYLQQLDIQRLRQSAREQRIWLGIAPHLVRPLAFVMPTFG
                     HGSRGPEALRLALLAYELLSRGGAPAAPRVPSGRVVGRAEAMALLPGLPRSDLNGAAI
                     WSDVQMLNADRLLLECVMDAAAEGAVVANHVEALGPHTRAGHGCEGP"
     gene            complement(619..1446)
                     /locus_tag="P5558_07265"
     CDS             complement(619..1446)
                     /locus_tag="P5558_07265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY24143.1"
                     /translation="MALDIRRPEPAAGPGDVESAAKERLRAYWNEHVTDWPIATAEPG
                     SRAFFAETEAYRFEKLDYLERLIDYGGQAGRDVLDVGCGLGNDTARFAQGGARVTGID
                     IAPRAIELSERNFGQRGLAGRFLVMDGEAMTFPEASFDFVYCHTILHFTPDPAAMIRE
                     IHRVLRPGGTALLMSVNRYSWMRLMHRLARVEIDHLQAPVFHWFSPDELMAMAAPFAE
                     RRLVHERFPVATKVHKGLKSRLFNTCFVGLFDLLPRALVRGSAHHLLVFARKAEIAP"
     gene            complement(1451..2677)
                     /locus_tag="P5558_07270"
     CDS             complement(1451..2677)
                     /locus_tag="P5558_07270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY24144.1"
                     /translation="MRTRVLVVAPQPFFTPRGTPFSVYYRTLVMAELGMDIDVLTYGR
                     GEDVAIPGVRIIRIPAFHWLGPVKIGPSMLKLWLDFFVVLWSIGCLIRHRYAIVHAHE
                     EAVFFLRFLKPIFQFKLIYDMHSSLPEQLRNYRFTTSRLLIGMFEMLERSCLEGADAV
                     ITICPELARYAEPLMPDPERHFLIENSIFEPVKLARPAASGEAATRPVELPAGRPCIF
                     YAGTFEVYQGLDTLVAAFALARETCPRAFLVLIGGTPAQVEAMRALADRSGIGEHTLV
                     LGRMPQALAARYAARASVLVSPRSHGINTPLKIYEQLASGIPLVATNILSHTQVLDDK
                     VALLVDPDAPSMAAGLVRALTDDALRARLSANARQLYRTAYSRPVYEGKMRAMLAALG
                     AKAWAGQGSVVRQGQT"
     gene            complement(2674..3345)
                     /locus_tag="P5558_07275"
     CDS             complement(2674..3345)
                     /locus_tag="P5558_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24145.1"
                     /translation="MLLFSEMPEAGVFAGIGACERVGDWAIRPTGEAVSLIAERPGAL
                     PLVLVAGRQIVTREGLEILILGAPGPFAESVDLATGVAMGQAAGGLAVLPWGFGKWWG
                     RRGRILDRFLATATPGTVFLGDNGGRFAGAGAPAQFALARRRCIPILPGSDPLPLPGD
                     AHRAGSYGFVLDGPLDSTRPAAALKARLLAMRDQPRGFGRLQPLWRFLANQVGMQWRK
                     RMPGS"
     gene            complement(3648..5273)
                     /locus_tag="P5558_07280"
     CDS             complement(3648..5273)
                     /locus_tag="P5558_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24146.1"
                     /translation="MPSRTFDLTVTEPMLEAFAALSGDRNALHMDAGFARRSRYRQRV
                     AHGMLAFALLGRLAEAHPEKEIRFRRLEGKFILPIALGNELRLTVDTRPGHERAADME
                     VGPEVPWEATWRNRADGLTVITATGSYVAGASPTRVPPKGSASLLAKPVAEADRGAEA
                     LADLVETLHCVVSADALASLARLIGLDPPLLPCPNLAATLLLSTLVGMRLPGRRATFT
                     SFRLDFVADLEPGRPAELEGRVTKVAVASETLQVAVRLTDAGRELATGEVRAVVNPAP
                     RVMLSASEIKGRHLGLGLEGRTVVVVGASRGIGETAAKLFAMHGARVVVHYFRGRADA
                     EAIVAEIGEAGGEAVALHCDIRDEAAVALFFAAVDEACGGLDVLVNNAVLDFRPRAWG
                     ELGWADYLGELDVSLRGLHACCGEAVSRFRAQGGGKIVNVSSIATSQPVRGQSRYITA
                     KSAIEGYTRSLAVELLKENIQVNMVVPAMTETDLIAAIPSDFVRRMAGARGMGRHVRP
                     IEVAQAMVYLASPWADALSGQAIVLNLGEPPFA"
     gene            5637..6668
                     /locus_tag="P5558_07285"
     CDS             5637..6668
                     /locus_tag="P5558_07285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY24147.1"
                     /translation="MPALVVGVVTCNSEEIIERTLAAIHAQDYPQVTVLVADNASTDR
                     TRELVTEQFPAVELIALAENNGPNPARNRIVERAGDGLALILDDDCIPTPGCFQRLVE
                     AALARPEAAAFGARVVYLSDPQRIQFDGALVHYLGEAVQLNGGRRVGDVADATHEVTG
                     LGAGCMLIRGAAWREVGGFDEYLVFGREDGELVQRLLIAGHRAFVVPSAVVLHDYTPR
                     ALSAGFYQVRNRWLVMLGLYRLRTLLLLAPALLVHELAMAAFLLAQGEFGTYLRANLA
                     VLRGLPQVRAKRRQVAAFRRVPDSAFLGCGAITVRAELLERHGWRARVKRALDIFYAG
                     YWRLVRRFV"
     gene            complement(6807..8000)
                     /locus_tag="P5558_07290"
     CDS             complement(6807..8000)
                     /locus_tag="P5558_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY24148.1"
                     /translation="MPGRLRRAISGYLHEWQALRGCYPDFVLRDVDALPKGDVPVFTF
                     HTIEPEPFEAQLRHLRQNGYRTIDGAALLRHLAGLAPAPERSVMLTIDDGRKSVWTHG
                     LPLLRRYGFRATVFLIPGYVQDGQGVAASLDDVWAGRIAAADLAIADPALMNWNEIRA
                     LQASGVIDCQSHTRFHHRVPIDTAPGGFIGPTTRDAFFDLPLPSGEEWRVAGPGTAGA
                     YGMPLYPSASLMLARPRYHPPTAVVAACVDHVRRQGGAAYFARRDAAAQLRRVHDAAK
                     AAHGRGRMESPAEALAAIAEDLAGSRALIETALGGKPCTHLCFPYTEGTAAAVELARE
                     AGFEAAYWGVLPDRRINRPGDDPLRIVRLKNDYIHRLPGAGRRSLGRIMLAKVHRRLT
                     GGPIH"
     gene            8051..9100
                     /locus_tag="P5558_07295"
     CDS             8051..9100
                     /locus_tag="P5558_07295"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRY24149.1"
                     /translation="MTSTTPGADPQTGLRPIFVVGAARSGTTLLQSMIDAHPDIALVG
                     ELHFFDQILRLRETIPEPVDRRAIEPLRQGILTCHASRFVPDIDRALDLALPRLATAP
                     RPTYGLLFRLLLEAFAELRGAGRVGEKTPTNVRYLAEIVELFPDARVVHILRDPRDSI
                     SSRVRYPFSSPSVIFNTLLWKIEMIYAFDFRATEKVGDGRYLEVHYEALVQDTEQQLR
                     RICRFIDVHYAAKMLAGHRRADRVVRGEPWKAGVGEPVNRRSVDAWQQRLTPAQVGLI
                     EALGGACIEQSGYQRIATAGRPRLVVEALHDVLRYVPYKLRTSWRARREERAGTTIGS
                     ESSKITSMLWRALRS"
     gene            complement(9097..10095)
                     /locus_tag="P5558_07300"
     CDS             complement(9097..10095)
                     /locus_tag="P5558_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRY24150.1"
                     /translation="MNRSISFALKLGLGLGILALIVYFVDLGDTLRALHGADPGWLLL
                     SLACYVSTRVLMGVKWWVLLGGRGETVSYATVQRALLLSDYHGLLFPNTLAIDALRAV
                     LLRHHPRGWTHTAASIVADRVINIAVAALVALLALAVSTLLVAHPFTPRVTLAVLAIS
                     GSIVAAALAVASQRLFDLVITVLHAIAAHGPLKGVVGRVLRKLGELHGAMRTMLTNPQ
                     VVVQAVLLAVLLVLMRCLSAYFVFRAIGTPVGFGWILTLMPVITTIALLPLTLFGLGL
                     KDGAFVFFFSGIGVAASAALAVSFVTYALIIVTSVVLGLLASVIGPPLPVAAKGEG"
     gene            10303..10830
                     /locus_tag="P5558_07305"
     CDS             10303..10830
                     /locus_tag="P5558_07305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRY24151.1"
                     /translation="MNLATALRRFLVPAPVVSLYYFAKFGCKISTRAEVEVSPNVTIG
                     RGTQISSFVKIKASNGPLQIGERVTIATGVFIDAHKGGLSIGDHTMIGPNSVLVGVNY
                     RYDRLDAPIQTQGLVSKGIRIGKDVWLGANVVVTDGAVIGDHTIVGPGSVVTGSLPDR
                     VIASGAPAKVVFRRR"
     gene            10917..11663
                     /locus_tag="P5558_07310"
     CDS             10917..11663
                     /locus_tag="P5558_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY24152.1"
                     /translation="MTVGRSVAGSGRRGGWRDAAALGGSLLPAAIAALTLARVGGLPW
                     LPGVLLVGLVTSGVLATLHANGHRRPFGLANGVTLLRLDLAACLLAAVWVGPEPLPLG
                     ARPAWAVFALAALALALDGIDGWLARRRGEASAFGARFDMASDTFLAIVLALCAWRFV
                     GLAPWVLAIGLLRPAFVTAGLAWPRLAAPLPPSRWRRAACGTALLLLVMTLAPPLARV
                     APWLAAGSLLILLHSFGRDIRRLLDRRQTA"
     gene            11983..12243
                     /locus_tag="P5558_07315"
     CDS             11983..12243
                     /locus_tag="P5558_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRY24153.1"
                     /translation="MHQGSSSDDAYWKANLSLIRNILIIWALVSFGFGILLRPLLSGI
                     PVGGTDLGFWFAQQGSIIIFIILIFYYAIRMNAIDRKFGAEE"
     gene            12272..14047
                     /locus_tag="P5558_07320"
     CDS             12272..14047
                     /locus_tag="P5558_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRY24154.1"
                     /translation="MTTQTLIYLIVGATFALYIGIAIWARASTTGEFYAAGRGVHPVM
                     NGMATAADWMSAASFISMAGLIAFVGYDNSTYLMGWTGGYVLLACLLAPYLRKFGKYT
                     VPEFIGDRFYSQSARIIAVICLIVASVTYVIGQMTGVGVTFARFLQVEASTGLYIGAA
                     IVFAYAVLGGMKGITYTQVAQYIVLITAYTIPAIFISLQLTGTVLPPLGLFSDSVSSG
                     TPLLVELDGLITDLGFASYTGHHTNTLNMVLFTLSLMIGTAGLPHVIIRFFTVPKVAD
                     ARWSAGWALVFIALLYLTAPAVGAMARFNLVNTIQTGEVGTPEGNLAYADVPTWFTTW
                     EQTGLIKWEDKNEDGRIQFYNDKNTAFETQAEAWGWRGNELTVNADILVLANPEIAAL
                     PGWVIALVAAGGLAAALSTAAGLLLAISSAISHDLIKGTINPNISEGGELMAARVSMA
                     AAIAVATYLGLNPPGFAAQTVALAFGLAAASIFPALMMGIFFKRINNVGASLGMLAGL
                     LFTVIYIFVYKGWFFLPGTSQFPDTPEYWVFGISPLSIGAIGAVVNFAVAYFASQATQ
                     EPPQEIQVLVESIRVPRGAGAAIDH"
     gene            14124..15974
                     /locus_tag="P5558_07325"
     CDS             14124..15974
                     /locus_tag="P5558_07325"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRY24155.1"
                     /translation="MTGTVDIGQGLGSFLAQHHPFDLLPPEALDGLVAGGEIVTFGQG
                     DVLYEKGEPVTRFTIVLEGAIDVLSPDGELLNRFSPGEGIGARGMLRDGSGPNRAVAG
                     AETRALALPKAFFLELLATYPDFAHFFERLRDTADRKPIFAADTADSLITNQLGDIMT
                     RSPVSVAPTATVQAAAALMRDRGISCLPVADAKGLVGLLTTSDITGRVVARGLSAETP
                     VAEVMTAEPLALPPEALVFDALLLMGERHIGHLPIVDKGELVGILTRTNLIRRQSISV
                     AFLIGDINEATEIARLATVIADVPVFLAQLVGAGVEAFKVGQLVTSITDALTRRLLVL
                     AEARLGPAPVPWLWLACGSQGRREQTGFSDQDNCLILDDAYASEAHEAYFAELARFVS
                     DGLDACGYYYCPGDMMATNPRWRVPLRRWREYFRGWIAQPDPMAQMLSSVMFDLRPIA
                     GETALFAGLQRETLEAARKNSIFRAHMIANSLKHAPPLSLFQGFALIRSGEHRNTLDL
                     KLSGVVPIVDLARVYALDGGIEEVNTRERLIAARAAGSLSEAGAGDLVDAYDLISRIR
                     LEHQARQVRDGNRPDNFMAPASLSALERRYLKDAFGVVKSMQAALRSRSA"
     gene            15987..16520
                     /locus_tag="P5558_07330"
     CDS             15987..16520
                     /locus_tag="P5558_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24156.1"
                     /translation="MIWNLIGTLVLGVAAASVVLVLFRVLGRPAPRWLLPAAAGIAML
                     GFHIWTDYSWFRRTTAELPGHVVVAQGYPSSNPLQPWTLVVPRIDRFSAVDTNRIRHN
                     PEVPDLAMAVVYLVARWNPTVEANQLYDCRTPRRADVSADMEVDAEGRPVDPVWVALP
                     PDDPVRLAVCRASPGQS"
     gene            16552..16938
                     /locus_tag="P5558_07335"
     CDS             16552..16938
                     /locus_tag="P5558_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY24157.1"
                     /translation="MAANTILVVDDEPGIRLYLKRLLGTDGFTVQTAADGDEALASIE
                     AAPPDLVLLDVMVGGRDGYEICEAIKRDPEKRAIKVCMLSAKSRAIEIEKGMAMGADA
                     YLTKPFSMDELRATVRDLLGRGKGEP"
     gene            16935..19118
                     /locus_tag="P5558_07340"
     CDS             16935..19118
                     /locus_tag="P5558_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRY24158.1"
                     /translation="MSERPGSERPRSESTGLRLRFFLFFALIGIGGALAVIGAMVLLA
                     RQGEALRLDHFVLFGGAAAAVVLGLATWVWLKFDERVARPLLAVANHLRAAAHARAER
                     AMPAGTARDLGPLGPAVGELTAAMAEARREVDRTIAEATARTEEQRQQLATILRDLQQ
                     GVVICNLEHQVLLYNQRALEILHVAGDIGLGRSFFGVVTAQPFRHALVRLANRFSSGR
                     HTDHPDGLACLVIGATKLGNYTLKGRVSLTVGRDPAGPEGERAERPVGYVVIFEDVTD
                     ELAAGIWRDRMLHDVTSDMRQRIANLGMIGDLLTLGEPIDADELAAAREAFLADGRIL
                     AERLDRLDEISSDLLSSAWPMTQVHSKTLLDLIVDRRSEGRNLAVETGGDLVWLHCDS
                     ASVVDLVDRLLNRTAVHAGITTFSLAVTRNERRAYLDLAWVGAPVPVRELEAWLEERL
                     DEGLGAITGRDVLNRHKTDLWCEPAGDGGARLRLPLTTVAIDTNRSRGAVRLESRPEF
                     YDFDLFGRSVPADLGEAPLRALTFVVFDTETTGLEPSGGDKIVQLAGVRIVNGRLLRG
                     EVFNSLVHPGRPIPSVAAKVHGITDAMVATADPIGAVLPRFHRFVGDAVLVAHNAAFD
                     MKFLTLEQDATGVVFTNPVLDTVLLGAAVFGAEESLTLDTLAERFDIRIASEERHTAL
                     GDALATGHTLLKLLDLLEAAGVRTLKQAFEASEQQAAIRRAQGKY"
     gene            19301..20158
                     /locus_tag="P5558_07345"
     CDS             19301..20158
                     /locus_tag="P5558_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY24159.1"
                     /translation="MKVFATRVWGFDPANWPVINFGLAGNRSKLLNESAAGDRIVFVA
                     TQSPPTAESDRGRLLGMAEIGREPVDTLDVLRPDVPRPEDYDDAGRFRWPKGLLMIRA
                     WAFDPSPLLRDVLAAQLPYNATPQAVMLSERDAAAVLSLTRKEIAIPESPAQERARLL
                     DAALRANRPTTGPRPVAWQGETNRTTDQPAVTYALRFGDSDIWKIGHAVDLEKRVKEI
                     NWHIPNEIIADAWGPALQQKWPNQESAFAMEQRVLGLLSERRTRAERLRCPKSELERA
                     WTRAIGIGI"
     gene            complement(20207..21331)
                     /gene="modC"
                     /locus_tag="P5558_07350"
     CDS             complement(20207..21331)
                     /gene="modC"
                     /locus_tag="P5558_07350"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HRY24160.1"
                     /translation="MSIDVAINHRLGGFTIDATFRSGAGLTALFGRSGAGKTTVVNAV
                     AGLLRPEQGRVTVDGQVLLDTQRGIVVPPHRRRIGYVFQEGRLFPHLSVRQNLGFGRW
                     FQRHQHRFGSFADTVELLGIGHLLERRPGSLSGGEKQRVAIGRALLASPRLLLMDEPL
                     ASLDEARKAEILPYIERLRDAFGLPILYVSHSVPEIARLATTVVVLADGRVERCGPPA
                     ALMADPDLFPLMGRHEGGALLAARILAHDPIDELTELEVTGGRLWVPLVEAPIGTQLR
                     VRIRARDVILAAAPPVGLSALNCLPATVVEIGALDQPIVDVKLVSGDDFILARITRRS
                     LRVLALEPGRPVHAVLKSIAVGRRDIGRDEHLAEGGSGKD"
     gene            complement(21345..>21577)
                     /locus_tag="P5558_07355"
     CDS             complement(21345..>21577)
                     /locus_tag="P5558_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRY24161.1"
                     /translation="LAFAKAMGEFGATITFVSSIPGETQTIPSAIYALIQVPGGESGA
                     LRLTAVSIAVAMAALLASEAVARLVARRIADR"
BASE COUNT         3312 a         7583 c         7416 g         3266 t
ORIGIN      
        1 ccgggccctc gcacccgtga cccgctcggg tgtgagggcc cagggcctcg acatggttgg
       61 cgaccacggc accctccgcc gccgcgtcca tgacgcattc gagcagcagc cggtcggcgt
      121 tcagcatctg cacgtcggac cagatggcgg caccgttcag atcgctgcgc ggcaggcccg
      181 gcaggagcgc catcgcctcg gcccggccga ccacccggcc gctcggcacg cgcggggcgg
      241 cgggtgctcc gccgcgcgac aggagctcgt aggcgaggag cgccaggcgc agcgcctcgg
      301 ggccgcgcga gccgtggccg aaggtcggca tgacgaaggc gagcggccgg accagatgcg
      361 gggcgatgcc gagccagatc cgctgctcgc gcgcggactg ccgcaaacgc tggatgtcga
      421 gctgctgcag gtagcgcagc ccgccatgga tcagcttgaa ggagttgtgg gaggtcaggc
      481 cgccgaagtc gccgcgctcg accagcgccg tccgcagccc gcgctgcgcc gcgtcgcggg
      541 cgatgcaggc accatagatg ccgccgccga tgaccagcac gtcgagctcg ggcccggcca
      601 ggcggcccgg atcgcgcttc acggtgcgat ctccgccttc ctcgcgaaga ccaggagatg
      661 atgcgcagag ccccggacca gcgcccgcgg gaggaggtcg aagaggccga cgaagcaggt
      721 gttgaagagc ctgctcttga ggcccttgtg caccttcgtc gccaccggga accgctcgtg
      781 gacgaggcgc cgctcggcga agggtgcggc catggccatc agctcgtccg ggctgaacca
      841 gtggaacacc ggtgcctgca ggtggtcgat ctcgaccctg gcgagccgat gcatcagccg
      901 catccaggaa taccggttga ccgacatgag gagtgcggtg ccgccgggcc gcagcacccg
      961 atggatctcc cggatcatcg ccgcgggatc gggcgtgaaa tgcaggatgg tgtggcagta
     1021 gacgaagtcg aagctcgcct cgggaaaggt catggcctcg ccgtccatga cgaggaaccg
     1081 gccggcgagc ccgcgctgcc cgaaattgcg ctcgctgagc tcgatggcac ggggggcgat
     1141 gtcgatgccg gtgaccctgg caccgccctg ggcgaagcgc gccgtgtcgt tgccgagccc
     1201 gcagccgacg tcgagcacgt ccctgcccgc ctggccgcca tagtcgatca gccgctcgag
     1261 atagtcgagc ttctcgaagc gataggcctc ggtctcggcg aagaacgccc ggctgcccgg
     1321 ctcggccgtg gcgatcggcc agtcggtgac gtgctcgttc caataggcgc gcagccgctc
     1381 cttcgccgcg ctctcgacgt cgccgggccc ggcggccggc tccggtcgac ggatgtcgag
     1441 ggccatcggc tcaggtctga ccttgccgga cgaccgagcc ttgcccggcc caggccttgg
     1501 cccctagagc ggcgagcatc gcgcgcatct tgccttcgta gaccggtcgc gaataggcgg
     1561 tccgatagag ctgccgggcg ttcgccgaga gccgtgcgcg gagcgcgtcg tcggtgagag
     1621 cccgaaccag ccccgccgcc atcgacgggg cgtcggggtc gaccaggagg gcgaccttgt
     1681 cgtcgagcac ctgggtgtgc gagaggatgt tggtcgcgac caggggaatg ccgctcgcca
     1741 gctgctcgta gatcttgagc ggcgtgttga tcccgtggct gcgtggcgag accagcacgc
     1801 tcgcccgggc cgcgtagcgg gccgccagcg cctgtggcat ccgcccgagg accagggtat
     1861 gctcgccgat tccgctgcgg tcggccaggg cacgcatggc ctcgacctgt gccggggtcc
     1921 cgccgatcag gacgaggaag gctcttgggc aggtctcgcg cgccagggcg aaggccgcga
     1981 ccagcgtgtc cagcccctga tagacctcga aggtgccggc atagaagatg caggggcgac
     2041 cggccggcag ctcgaccggg cgcgtggccg cctcgccgct cgccgccggc cgggccagct
     2101 tcaccggctc gaagatgctg ttctcgatca ggaaatggcg ctcgggatcg ggcatcagcg
     2161 gctcggcgta gcgggcgagc tcggggcaga tggtgatgac ggcatccgca ccctcgaggc
     2221 agctccgctc cagcatctcg aacatcccga tcagcagccg tgacgtggtg aagcggtagt
     2281 tgcgcagctg ctccggcagg ctcgagtgca tgtcgtagat cagcttgaac tggaagatcg
     2341 gcttcaggaa gcgcaggaag aagaccgcct cctcgtgcgc gtgcacgatg gcgtagcgat
     2401 ggcggatcag gcagccgatg gaccagagca cgacgaagaa atcgagccag agcttcagca
     2461 tcgacgggcc gatcttgacc ggcccgagcc aatggaaggc gggaatgcga atgatccgca
     2521 cgcccggaat ggcgacatcc tcgccccggc cataggtcag cacgtcgatg tccatgccga
     2581 gctcggccat gaccagcgtg cggtagtaga cgctgaacgg cgtgccgcgc ggcgtgaaga
     2641 aaggctgcgg cgccacgacg aggacgcggg tcctcacgag cccggcatcc gcttgcgcca
     2701 ctgcatgccg acctggttgg cgagaaagcg ccagaggggc tgcagccgcc cgaagccccg
     2761 cggctggtcg cgcatcgcga gcagccgcgc cttgagcgcc gcggcgggcc gggtcgaatc
     2821 gagcgggccg tcgagcacga acccgtagct gccggcgcga tgggcgtcgc cggggagtgg
     2881 cagaggatcg gagcccggca ggatcggtat gcagcgtcgg cgcgccaggg cgaattgggc
     2941 gggtgccccg gcaccggcga agcggccgcc attgtcgccg aggaagaccg tgcccggggt
     3001 ggcggtcgcg aggaaccggt cgaggatccg gccgcgccgg ccccaccatt tgccgaagcc
     3061 ccagggcagc acggcgaggc cgccggccgc ctggcccatg gcgaccccgg tcgccagatc
     3121 gacggattcg gcaaagggac cgggcgcacc caggatcagg atctcgagcc cttcccgcgt
     3181 cacgatctgg cgacccgcga cgaggacgag cggcaaggcg cccggccgct cggcgatgag
     3241 cgagacggcc tcgccggtcg gccggatcgc ccagtcaccg acccgctcgc aggcaccgat
     3301 cccggcgaac acgcccgcct cgggcatctc gctgaagagc agcaccccga ccgccggccg
     3361 cagcccgcaa gcggttgcgc ccgcgcagaa atttcgcgcg gcgtggtcga ggaacgccac
     3421 cggcccgagg ttgcgatggc aatgcacatg tgcatcgaca aggagtattc cgtcccaact
     3481 caacggccga ggccccgtcc ctaatcgagg gtaataccga ccccagtccc ccggtcaacc
     3541 agcatccgtg gattcggcag cgcagccaca gcgaccttcc gccagcgctg cacgatgtta
     3601 catactcgga ggaaaagtgc cacgctcaca ggtgtgtcga tgctgcatca ggcgaacggc
     3661 ggctcaccca gattgagcac gatcgcctgg cccgacaagg cgtccgccca gggcgaagcg
     3721 aggtagacca tcgcctgggc gacctcgatc ggccgcacat gccgccccat gccccgggcc
     3781 cccgccatgc ggcgaacgaa atcgctcggg atggcggcga tgagatctgt ttcggtcatg
     3841 gcgggcacca ccatgttgac ctggatattc tccttcagga gctcgacggc gaggctcctc
     3901 gtatagcctt cgatggcgct cttcgccgtg atgtaccggc tctggccacg caccggctgg
     3961 ctcgtcgcga tgctcgagac gttgacgatc ttgccgccgc cctgcgcccg gaaccggctg
     4021 accgcctcgc cgcagcaggc atgcaggccc ctgagcgaga cgtcgagctc gcccagatag
     4081 tcggcccagc cgagctcgcc ccacgcccgg gggcggaaat ccaggaccgc gttgttgacc
     4141 agcacgtcga gcccgccgca ggcctcgtcg accgcggcga agaacagcgc caccgccgcc
     4201 tcgtcgcgaa tatcgcaatg gagcgccacg gcctcgccac cagcctcgcc gatctcggcg
     4261 acgatcgcct cggcgtcggc cctgccgcga aaataatgca cgacgacccg ggcaccgtgc
     4321 atggcgaaga gcttggcggc ggtctcgccg attccccggc tggcgccgac cacgacgacg
     4381 gtccggccct cgagcccgag cccgagatgg cggcccttga tctcgctcgc cgacagcatg
     4441 acacgcggcg ccgggttgac cacggcgcgg acctcgccgg tcgcgagctc gcgccccgca
     4501 tcggtcaggc ggacggccac ctgcagcgtc tcgctcgcga ccgcgacctt cgtcacgcgc
     4561 ccctccagct cggccgggcg gccgggctcg aggtcggcca cgaagtcgag gcgaaagctc
     4621 gtgaaggtgg cacgccggcc gggcaggcgc atgccgacca gggtcgagag gaggagcgtc
     4681 gccgcgaggt tcgggcaggg cagcagcggc gggtcgagcc cgatcagccg ggcgaggctg
     4741 gccagggcat cggccgaaac gacgcagtgc agcgtctcga ccaggtcggc gagcgcctcg
     4801 gcgccccgat cggcttcggc cacgggcttt gccaggagcg aggcggaacc cttcggcggg
     4861 acgcgcgttg gcgaagcacc ggcgacgtag ctgcccgtgg cggtgatcac ggtcaggcca
     4921 tcggcgcgat tgcgccacgt cgcctcccag ggcacctcgg ggccgacctc catgtcggcc
     4981 gcacgttcat ggcccggccg ggtgtcgacc gtgaggcgca gctcgttgcc gagcgcgatc
     5041 gggaggatga acttgccttc gagccggcgg aacctaattt ccttttctgg atgcgcttcg
     5101 gccagccggc cgagcagggc gaaggcgagc atgccgtgcg cgactcgctg ccgatatcgg
     5161 ctgcgacggg cgaaccctgc atccatgtgc agcgcgttgc ggtcgccgct cagcgcggcg
     5221 aaagcctcga gcatcggctc ggtgacggtc agatcgaacg tgcgagaggg caaacgtggc
     5281 ctccggtgga ggacggccgc aggcggtggc tgtcccggct gcctagcggg ttccgcggtc
     5341 gccgccaagc ccgcgggcgg cggggccggc gtgcggtggc ccgaccaggc agcgttgcaa
     5401 cagggacgca agtcccggtc attcggcggg aagtgtaccc aatggtagtc gtgaagggat
     5461 cgaggatcgc gtccgcgtgg gtgcgtgacc ccggccctca tgacaggatt caatcgcggg
     5521 caaaatgcga tgtgacgctc gatattgctt acgaccggga gggaaatgtg gccagggcgg
     5581 atgcgcgcgg gcagtcatcc gcacaccagc cgacaggtgc tgatgaaggc cggggattgc
     5641 cggcgctcgt cgtcggggtg gtcacgtgca acagcgagga gatcatcgag cggacactgg
     5701 ccgcaatcca cgcccaggat tacccgcagg tcacggtgct ggtcgccgac aatgcctcga
     5761 ccgatcggac ccgggagctg gtcaccgagc agttcccggc ggtcgagctc atcgcgctgg
     5821 ccgagaacaa cggccccaat cccgcccgca accgcatcgt cgagcgcgcc ggcgacggtc
     5881 tggcgctgat tctcgacgac gactgcatcc ccacgcccgg ctgctttcag cggctggtcg
     5941 aggcagcgct ggcccggccc gaggccgcgg ccttcggcgc ccgcgtggtc tacctgagcg
     6001 atccgcagcg catccagttc gacggcgcgc tcgttcacta cctgggcgag gccgtgcagc
     6061 tcaatggcgg ccggcgggtc ggcgacgtgg ccgacgcgac gcacgaggtc accggcctcg
     6121 gtgccggttg catgctgatc cgtggtgcgg catggcgcga ggtcggcggg ttcgacgaat
     6181 atctggtctt cggccgcgag gacggcgagc tggtgcagcg cctgctgatt gccgggcacc
     6241 gggcatttgt cgtgccgagc gccgtggtgc tccacgacta cacgccccgc gcgctctccg
     6301 ccggcttcta tcaggtccgc aaccgctggc tggtcatgct cgggctctac cggctgcgca
     6361 cactgctcct gctggccccg gcgctgctcg tccacgagct cgcgatggct gcctttctcc
     6421 tggcgcaggg cgagttcggc acctatctgc gcgccaatct ggccgtgctg cgcggcctcc
     6481 cgcaggtgcg cgcgaaacgt cggcaggtcg ccgccttccg ccgtgtcccc gacagcgcct
     6541 ttctcggctg cggggcgatc accgtccgtg ccgagctgct cgagcgccat ggctggaggg
     6601 cccgggtcaa gcgggcgctc gacatcttct atgccggcta ctggcgcctg gtgcgccgat
     6661 tcgtctagag cgctttcacc atgaccggat acttcgtcag gctgcggacg atcggtgctc
     6721 acgcaggtag accacgctgc gcgccgatcg ccccggtcag tccaatggcc cttcctcgtc
     6781 cacggtcacc gtgaaagcgc gccaggtcag tggatggggc cacccgtgag gcgtcggtgg
     6841 acctttgcca gcatgatccg gccgagcgag cggcggccgg cgcccggcag ccggtggatg
     6901 tagtcgttct tcagccggac gatccgcagc gggtcgtcgc cgggccggtt gatccggcga
     6961 tccggcagca cgccccaata ggccgcctcg aagccggcct cacgggccag ctcgacggct
     7021 gcggccgtgc cttcggtata ggggaagcag agatgggtgc agggcttgcc gccgagcgcc
     7081 gtttcgatca gggcgcgcga gcccgccaga tcctcggcga tggcggccag cgcctcggcc
     7141 gggctctcca tccgcccccg gccgtgcgcc gccttcgccg cgtcgtggac ccggcgcagt
     7201 tgcgccgcgg cgtcccgcct ggcgaaatag gccgccccac cctggcgccg cacgtgatcg
     7261 acgcaggccg ccacgaccgc ggtcggcgga tgatagcgcg gccgggcgag catgagcgac
     7321 gccgagggat agagcggcat cccgtaggca ccggccgtcc cgggcccggc gacccgccat
     7381 tcctccccgc tcggcaaggg caggtcgaag aaggcgtccc gcgtcgtcgg gccgatgaag
     7441 ccgccgggtg ccgtgtcgat cggcacgcga tggtgaaagc gcgtgtgcga ctggcagtcg
     7501 ataacgccgg atgcctgcag cgcgcggatc tcgttccagt tcatcagcgc cggatcggcg
     7561 atcgcgaggt cggccgcggc gatcctgccg gcccagacgt cgtcgaggct ggcggcgacg
     7621 ccctgcccat cctggacata gcccgggatg aggaacacgg tggcccggaa gccgtagcgc
     7681 cgcaacagcg ggagcccgtg cgtccagaca ctcttgcgac cgtcgtcgat ggtcagcatg
     7741 accgagcgct ccggcgccgg agcgaggccg gcgagatgcc gcaacagggc ggcgccgtcg
     7801 atggtccggt agccgttctg ccggagatgg cgcagctgcg cctcgaacgg ctcgggctcg
     7861 atcgtatgga acgtgaagac gggaacgtcg cccttgggca gcgcatcgac atcgcgcagc
     7921 acgaagtcgg gatagcagcc gcgcagcgcc tgccattcgt gcagatagcc gctgatcgcc
     7981 cgcctcagtc gccccggcat cacccttccc cgacaccctt cctgccgact gcctgccgga
     8041 gggcgtctcg atgaccagca ccacccctgg cgccgacccg caaacggggc ttcgaccgat
     8101 cttcgtcgtc ggcgcggccc gctccgggac gacgctcctg cagtcgatga tcgatgccca
     8161 tccggatata gcactggtcg gcgagctgca cttcttcgat cagatcctgc gcctgcgcga
     8221 gaccatcccc gagcccgtcg accgccgggc gatcgagcct ctgcgccagg gcattctcac
     8281 ctgccatgcc agccggttcg tgccggacat cgacagggcg ctcgatctcg ccctgccccg
     8341 cctcgccaca gccccgaggc cgacctacgg cctgctcttc aggctgctgc tcgaggcgtt
     8401 cgcggagctg cgcggcgccg ggcgcgtcgg cgagaagaca ccgaccaacg tccgctatct
     8461 cgccgagatc gtcgagctct ttccggatgc gcgggtcgtc cacatcctgc gcgatcccag
     8521 ggattccatc tcctcccggg tccgctaccc gttctcctcg ccgtcggtga tcttcaacac
     8581 gctgctctgg aagatcgaga tgatctacgc gttcgacttc agggcgaccg agaaggtggg
     8641 cgatggacgg tacctggagg tccactacga ggcgctcgtc caggataccg agcagcagtt
     8701 gcggcggatc tgccggttca tcgacgtcca ctacgcggcg aagatgctcg cgggccatcg
     8761 gcgggccgac cgggtggtca ggggcgagcc ctggaaggcc ggggtcgggg agccggtcaa
     8821 tcgccggtcg gtcgacgcct ggcagcagcg tctgacgccg gcccaggtcg gattgatcga
     8881 ggccctgggt ggtgcctgca tcgagcagag cggctaccag cgcatcgcca ccgctggccg
     8941 cccgaggctg gtcgtcgagg cgctgcacga tgtgctgcgc tacgtcccct acaagctccg
     9001 cacgagctgg cgggcccggc gcgaggagcg ggccggcacg accatcggca gcgaatcgtc
     9061 gaagatcacc tcgatgctgt ggcgggccct gcgttcctag ccttcgccct tggccgcgac
     9121 cggcaagggc gggccgatga cgctcgccag caggccgagc acgacgctcg tgacgatgat
     9181 cagcgcatag gtcacgaagg acacggcgag ggcggcgctc gccgcgaccc cgatgccgct
     9241 gaagaagaag acgaaggcgc cgtccttcag gccgaggccg aagagcgtca gcggcaggag
     9301 cgcgatggtc gtgatgaccg gcatcagcgt gaggatccag ccgaacccga ccggcgtgcc
     9361 gatcgcgcga aagacgaaat aggccgagag gcagcgcatc agcacgagca ggacggccag
     9421 cagcacggcc tgcaccacga cctgcgggtt ggtgagcatc gtgcgcatgg cgccgtgcag
     9481 ctcgccgagc ttgcgcagga cgcggccgac cacccccttc agcggtccgt gcgcggcgat
     9541 ggcatggagc acggtgatga ccaggtcgaa gaggcgctgc gaggcgaccg cgagcgccgc
     9601 cgccacgatc gagccggaga tcgcgagcac cgccagggtg acgcgcggcg tgaacgggtg
     9661 cgccaccagg agggtggaga ccgccagcgc caggagagcc accagggcgg cgacggcgat
     9721 gttgatcacc cgatcggcga cgatcgaggc ggcggtgtgg gtccagccgc gcgggtggtg
     9781 gcgcagcagc accgcgcgca gggcatcgat cgccagcgtg ttggggaaga ggagcccgtg
     9841 atagtcggaa agcaggagcg cccgctgcac ggtggcatag gagacggtct cgccgcgccc
     9901 gccgagcagg acccaccatt tcacccccat cagcacgcgc gtcgagacgt agcaggcaag
     9961 cgagagcagc agccagccgg gatcggcgcc gtgcagggcg cgcagggtgt cgccgagatc
    10021 cacgaaatac acgatcaggg cgaggatgcc caatcccagg ccgagcttca gcgcgaagga
    10081 gatcgaccgg ttcacgagcg cgcctcgtgg gcccggcgtt cccggggccc gtcgacggct
    10141 acgcgagcga cccgcttcag ggggacggtc acgccggcat cttcatgcgg atgtgctccc
    10201 tttagggccg gggcgggtcg gtgctgccgg cggcgcccga tcttgtcggc tcgttgtggc
    10261 cccactatcg atgggccacc cggcaatggc aagagtccgc ccatgaacct cgcaactgcg
    10321 ctccgccgct tcctcgtccc cgcgccggtg gtcagtctct actacttcgc caagttcgga
    10381 tgcaagatca gcactcgcgc cgaggttgag gtttctccga acgtgacgat cgggcgcggc
    10441 acccagatca gctccttcgt caagatcaag gcgagcaacg gcccgctgca gatcggcgag
    10501 cgggtgacga tcgccacggg cgtcttcatc gatgcgcaca agggcgggct cagcatcggc
    10561 gatcacacga tgatcggccc caacagcgtg ctggtcggcg tcaactaccg ctacgaccgg
    10621 ctggacgcgc cgatccagac gcaggggctg gtctccaagg gcatccggat cggcaaggat
    10681 gtgtggctcg gcgccaatgt ggtggtcacc gatggcgccg tgatcggcga tcacacgatc
    10741 gtcggccccg gctcggtcgt caccggcagc ctgccggacc gggtgattgc cagcggcgcg
    10801 ccggcgaagg tcgtttttcg ccgccgctga atgggagccc ggcaccgcct tgccggtttc
    10861 ccctgatggc tgcaccctcg ccgccggcga gggcgggcgg ccggtggacg cggggcatga
    10921 ctgtcggaag atcggtggcc gggtcgggac ggcgcggcgg ctggcgggat gcggcggcgc
    10981 tcggcggctc gctgctgccg gcagcgatcg ccgccctgac gctggcccgg gtcggcggcc
    11041 tgccgtggtt gcccggcgtg ctgctggtcg ggctggtcac ctcgggcgtg ctggccacgt
    11101 tgcacgccaa cggccaccgc cggcccttcg gcctcgccaa cggcgtcacg cttctgcgtc
    11161 tcgacctcgc cgcgtgcctt ctcgctgccg tgtgggtggg gccggaaccc ctgccgctcg
    11221 gcgcacggcc ggcatgggcg gtgttcgcgc tcgcggcgct ggccctggcg ctcgacggga
    11281 tcgacggctg gctcgcccgg cggcggggcg aggccagcgc gttcggcgcg cgcttcgaca
    11341 tggccagcga caccttcctc gccattgtcc ttgcgctctg cgcctggcgg ttcgtcgggc
    11401 tcgccccctg ggtgctggcc atcggcctcc tccgcccggc cttcgtgacc gccggtctgg
    11461 cctggccgcg gctcgccgcg cccctgcccc cgagccgatg gcgtcgggcg gcctgcggca
    11521 cggccctgct gctgctggtc atgaccctgg cgccgccact ggcccgggtc gcgccctggc
    11581 tcgccgccgg cagcctcctg atcctgctgc actccttcgg ccgcgatatc cgccgcctgc
    11641 tcgaccggcg gcaaaccgcc tgagccacca atggcgccga gtccgcggcg gaaatcgtcc
    11701 agcctgcgtt tttttgccgc gttgcaggct gcccgccgtc cgaggcgggc cggaatccgc
    11761 ccggtttcac ggcattcgcg gggcgctggg gccttcccgc ccgccccgct tggcccgtgg
    11821 ggcgcggcca gccggggcgt accggcagcc ccctgcgcgc tttagaccag tttccgccgt
    11881 catcgactgc cgcgacactt gcctgcagac gccgatgggc cgatcgaaac ctgcacggcg
    11941 caggtcacca tctgccgcag gcaaagacga ctaggaggca tcatgcatca gggatcatca
    12001 tccgacgacg cctattggaa ggccaacctc agcctgatcc gcaatatcct gatcatctgg
    12061 gcgctggtct cgttcggctt cggcattctc ttgcggccgc tgctctcggg cattcccgtg
    12121 ggcggcaccg acctgggctt ctggttcgcc cagcagggct cgatcatcat cttcatcatc
    12181 ctgatcttct attacgccat caggatgaat gcgatcgacc gcaagttcgg cgctgaagag
    12241 tgatcgtggc ggaacgaggg aaaagcagaa catgaccacc cagacactca tctacctcat
    12301 agtcggtgcc acattcgctc tctatatcgg catcgccatc tgggcccgcg ccagcacgac
    12361 cggcgagttc tacgccgccg gccggggcgt ccatcccgtc atgaacggca tggcgacggc
    12421 cgcggactgg atgtccgccg cctccttcat ctcgatggcc ggcctgatcg ccttcgtcgg
    12481 ctacgacaac tcgacctacc tgatgggctg gaccggcggc tacgtgctgc tcgcctgcct
    12541 gctcgcgccg tatttgcgca agttcggcaa gtacaccgtg cccgagttca tcggtgaccg
    12601 gttctactcg cagagcgcgc gcatcatcgc cgtgatctgc ctgatcgtcg cctcggtcac
    12661 ctacgtcatc ggccagatga ccggcgtcgg cgtcaccttc gcccgcttcc tccaggtcga
    12721 agcctcgacc ggcctctaca tcggtgcggc gatcgtcttc gcctatgccg tgctcggcgg
    12781 catgaagggc atcacctaca cgcaggtggc gcagtacatc gtgctgatca ccgcctacac
    12841 catcccggcg atcttcatct cgctgcagct cacgggcacg gtcctgccgc cgctcggcct
    12901 cttctccgac agcgtctcgt cgggcacgcc gctgctcgtc gagctcgacg ggctgatcac
    12961 cgatctcggc ttcgcctcct acaccgggca tcacaccaac acgctcaaca tggtgctctt
    13021 caccctgtcg ctgatgatcg gcacggccgg cctgccgcat gtcatcatcc gcttcttcac
    13081 cgtgccgaag gtggcggacg cccgctggtc ggccggctgg gcgctggtct tcatcgccct
    13141 cctctacctg acggccccgg ccgtcggtgc gatggcccgc ttcaacctgg tcaacaccat
    13201 ccagaccggc gaggtcggca ccccggaagg caacctcgcc tatgccgacg tgccgacctg
    13261 gttcacgacc tgggaacaga ccggcctgat caagtgggag gacaagaacg aggacggccg
    13321 catccagttc tacaacgaca agaacacggc cttcgagacc caggccgagg catggggctg
    13381 gcgcggcaac gagctgaccg tcaacgccga cattctcgtg ctcgccaacc cggagatcgc
    13441 ggccctgccg ggctgggtca tcgccctcgt cgccgccggc ggtctcgccg ccgccctctc
    13501 gaccgcggcc gggctcctgc tcgccatctc ctcggcgatc agccacgacc tgatcaaggg
    13561 gaccatcaac cccaacatct cggagggtgg cgaattgatg gcggcgcgcg tctcgatggc
    13621 cgcggccatc gcggtggcga cctatctcgg cctcaacccc ccgggcttcg cggcccagac
    13681 ggtggcgctg gccttcggtc tcgccgcggc atcgatcttc cccgccttga tgatgggcat
    13741 cttcttcaag cgcatcaaca atgtgggcgc cagcctcggc atgctcgccg gcctcttgtt
    13801 caccgtcatc tacatcttcg tctacaaggg ctggttcttc ctgccgggca cgtcccagtt
    13861 cccggacacg cccgaatact gggtcttcgg catctcgccg ttgtcgatcg gcgccatcgg
    13921 tgccgtggtc aacttcgcgg tcgcctactt cgcctcccag gcgacccagg agccgccgca
    13981 ggagatccag gtgctcgtcg agagcatccg ggtgccgcgt ggcgccggtg cggcgatcga
    14041 tcactgatcg gcagccactt cggttgacgt gatccgcgga ccccgcccat ctggacgggg
    14101 tccgcatctt tttcggagac agcatgaccg gcacggtcga tatagggcag ggtctcgggt
    14161 cgttcctggc ccagcaccat cccttcgacc tcttgccgcc cgaggcgctc gacgggctgg
    14221 tggcgggcgg cgagatcgtg accttcggcc agggcgacgt gctctacgag aagggtgagc
    14281 cggtcacaag gttcacgatc gtcctcgagg gtgcgatcga cgtcctctcg cccgacggcg
    14341 agctgctcaa ccgcttctcg cccggcgagg gcatcggcgc gcgcggcatg ctgagggacg
    14401 gcagcggccc gaaccgcgcc gtcgcgggcg ccgaaacgcg ggccctcgcc ttgccgaagg
    14461 cgttctttct cgagctgctc gcgacctatc ccgatttcgc ccatttcttc gagcggttgc
    14521 gcgacactgc cgaccgcaag ccgatctttg ccgccgacac cgcggattcg ctgatcacca
    14581 accagctcgg cgacatcatg acgaggtcgc cggtcagcgt ggcgccgacc gccaccgtcc
    14641 aggcagcggc ggcgctgatg cgcgaccgtg gcatctcctg cctgccggtg gccgacgcca
    14701 aaggtctcgt cggcctcctg acgaccagcg acatcaccgg ccgcgtggtt gcgaggggcc
    14761 tttcggccga gacgccggtg gccgaggtga tgaccgccga gcccctggcc ctgccgcccg
    14821 aggcgctcgt cttcgatgcg ctgctcctca tgggcgagcg gcatatcggg cacctgccga
    14881 tcgtcgacaa gggcgagctc gtcggcatcc tgacccgcac caacctgatc cgccgccagt
    14941 cgatctcggt ggccttcctc atcggcgaca tcaacgaggc gaccgagatc gcgcggctcg
    15001 ccaccgtcat cgccgacgtg ccggtgttcc tggcccagct cgtcggtgcc ggggtcgagg
    15061 ccttcaaggt gggccagctc gtcaccagca tcaccgatgc gctgaccagg cggctcctcg
    15121 tcctcgccga ggcgaggctc gggccggcgc ccgtgccctg gctctggctc gcctgcggca
    15181 gccagggccg gcgcgagcag accggcttct ccgaccagga caactgcctg atcctcgacg
    15241 acgcctatgc gagcgaggcg cacgaggcct atttcgccga gctcgcccgc ttcgtctccg
    15301 acgggctcga tgcctgcggc tactattatt gcccgggcga catgatggcg accaacccgc
    15361 gctggcgcgt gccgctccgg cgctggcgcg aatacttccg cggctggatc gcccagccgg
    15421 acccgatggc gcagatgctg tcctcggtga tgttcgatct gaggccgatc gccggcgaga
    15481 cggcgctctt cgccgggctg cagcgcgaga cgctggaggc ggcccgcaag aacagcatct
    15541 tccgcgcgca catgatcgcc aactcgctca agcacgcgcc gcccttgagc ctttttcagg
    15601 gcttcgccct gatccgctcg ggcgagcatc gcaacaccct cgacctcaag ctcagcggcg
    15661 tcgtgccgat cgtcgatctc gcccgggtct acgcgctcga tggcggcatc gaggaggtca
    15721 acacgcgcga gcggctgatc gcggcgcgcg cggcgggctc gctcagcgag gccggcgccg
    15781 gcgatctggt cgacgcctac gacctcatct cgcgcatccg cctcgagcac caggcccgcc
    15841 aggtccgcga cggcaacagg cccgacaact tcatggcgcc ggcctcgctc tcggcgctcg
    15901 agcggcgcta cctcaaggac gccttcggcg tggtcaagtc gatgcaggcg gcgctccgct
    15961 cgaggagcgc ataaggtgcc tgtccgatga tctggaattt gatcggcacc ctcgtcctcg
    16021 gcgttgccgc ggccagcgtg gtcctcgtcc tgttccgcgt gctcgggcgg ccggcaccgc
    16081 gctggctgct gccggcagca gcaggcatcg ccatgctcgg cttccacatc tggacggact
    16141 attcgtggtt caggcgcacc acggccgagc tgccgggcca cgtggtcgtg gcgcagggct
    16201 atccctcgag caacccgctg cagccctgga ccctcgttgt cccgcgcatc gaccggttca
    16261 gcgccgtcga caccaaccgc atccgccaca acccggaggt gccggacctg gccatggcgg
    16321 tggtctatct cgtcgcccgc tggaacccga ccgtcgaggc gaaccagctc tacgattgca
    16381 ggacgccccg ccgggcggat gtcagcgccg acatggaggt cgatgccgag ggccggccgg
    16441 tcgacccggt ctgggtggcg ctgccaccgg acgacccggt ccggctcgcg gtctgccggg
    16501 cgagcccggg ccaatcatga aaaaaccacc tccgatcagg gaacgtccat catggcggcc
    16561 aacacgatcc tcgtggtcga cgacgagccg ggcatccgcc tctatctgaa gcgcctgctc
    16621 ggcaccgacg gcttcaccgt ccagaccgcc gccgacggcg acgaagcgct cgcctcgatc
    16681 gaggcagcac cgccggacct cgtcctgctc gacgtcatgg tcggcggccg cgacggctac
    16741 gagatctgcg aggcgatcaa gcgcgatccg gagaagcgcg cgatcaaggt ctgcatgctt
    16801 tccgccaaga gccgtgcgat cgagatcgag aagggcatgg ccatgggtgc cgatgcctat
    16861 ctcaccaagc ccttctcgat ggacgagctc cgggcgaccg tgcgcgacct gctgggccgg
    16921 ggaaagggcg agccttgagc gagcggccgg ggagcgagag gccccggagc gagagcaccg
    16981 gtctccggct ccgcttcttc ctcttcttcg cgctgatcgg catcggcggt gcgctggcgg
    17041 tgatcggcgc catggtgctg ctcgcccgtc agggagaagc gctcaggctc gatcatttcg
    17101 tgctctttgg cggcgccgcc gcggcggtcg tgctcggcct cgccacctgg gtctggctca
    17161 agttcgacga gcgcgtggca cggccgctgc tggccgtcgc caatcacctg agggcggccg
    17221 cccacgccag ggccgagcgg gcgatgcccg cgggcacggc ccgcgatctc ggcccgctcg
    17281 gcccggcggt cggcgagctg acggcggcca tggccgaggc tcgccgcgag gtcgaccgga
    17341 ccatcgcgga ggcgaccgcg cggacggagg agcagcggca gcagctcgcc accatcctgc
    17401 gcgacctgca gcagggtgtc gtcatctgca acctcgagca tcaggtcctg ctctacaacc
    17461 agcgggcgct ggagatcctc cacgtggcgg gcgatatcgg cctcggccgc tcgttcttcg
    17521 gcgtggtcac cgcccagccc ttccgtcacg cgctggtgcg tctcgccaat cgtttcagca
    17581 gcggccggca caccgatcac ccggacggcc tcgcctgcct cgtcatcggc gccacgaagc
    17641 tcggcaacta cacgctcaag ggccgtgtct cgctcactgt cggccgcgac ccggccgggc
    17701 cggagggcga gcgcgcggag cggccggtcg gctatgtcgt catcttcgag gacgtcaccg
    17761 acgagctcgc cgccggcatc tggcgcgacc gcatgctgca cgacgtgacc agcgacatgc
    17821 gccagcggat cgccaatctc ggcatgatcg gcgatctgct caccctgggc gagccgatcg
    17881 atgcggacga gctggcagcg gcgcgcgagg ccttcctggc ggacggcagg atcctggcgg
    17941 agcggctcga ccggctcgac gagatctcct ccgatctcct ctcctccgcc tggccgatga
    18001 cccaggtgca ttccaagacc ctgctcgacc tgatcgtcga ccgccgctcg gaaggccgca
    18061 acctcgcggt cgagaccggt ggcgacctcg tctggctgca ctgcgacagc gcatcggtgg
    18121 tcgatctcgt cgaccgcctg ctcaaccgga cagcggtgca cgcgggaatc accaccttct
    18181 cgctcgccgt gacgcgcaac gagcgccggg cctatctaga cctcgcctgg gtcggggcac
    18241 ccgtgccggt gcgcgagctc gaggcctggc tggaggagcg gctcgacgag ggccttggtg
    18301 ccatcaccgg ccgcgacgtg ctcaaccgtc acaagaccga cctctggtgc gagcccgcgg
    18361 gcgacggcgg cgcccggctc cgcctgccgc tgacgacggt ggcgatcgac accaaccgtt
    18421 cgcgtggcgc cgtccgcctc gaatcgcggc ccgagttcta cgatttcgac ctcttcggcc
    18481 gcagcgtgcc ggccgatctc ggcgaggcgc cgctccgcgc cctcaccttc gtcgtcttcg
    18541 acaccgagac cacgggcctc gagccctcgg gcggcgacaa gatcgtgcag ctcgccgggg
    18601 tgcgcatcgt caacggccgg ctgctcaggg gcgaggtctt caacagcctc gtccatccgg
    18661 gccggccgat ccccagcgtc gccgccaagg tgcacggcat caccgacgcc atggtcgcca
    18721 cggccgaccc gataggagcc gtgctgcccc gcttccatcg cttcgtcggc gatgcggtcc
    18781 tggtcgcgca caacgccgcc ttcgacatga agtttctcac gctcgagcag gacgccaccg
    18841 gtgtcgtctt caccaacccc gtgctcgaca ccgtgctcct gggtgccgcc gtcttcggcg
    18901 ccgaggagag cctgaccctc gacaccctcg ccgagcggtt cgacatccgc atcgccagcg
    18961 aggagcgcca cacggccctc ggcgacgcgc tggccaccgg ccacaccctg ctcaagctcc
    19021 tcgacctcct cgaggcggcc ggcgtgcgca ccctgaagca ggccttcgag gcgtcggagc
    19081 agcaggccgc gatcagacgg gcgcagggga agtattgagg gggtagggcg gcgtcgcggg
    19141 cgttgccgcg tctgcggtcg gtgcaaccga atcgaccaag cgcattccgc tggaattgtc
    19201 gataaccgac gaactataat tcaacgataa gttgatattc agcgtgacga actgcccgtt
    19261 gccgtcgctg gcagcgctcg aagtgtcgag agggtcgacc atgaaggttt tcgcgacgcg
    19321 ggtctggggt ttcgacccgg ccaactggcc ggtcatcaac ttcggcctgg caggcaaccg
    19381 atccaagctg ttgaacgaat ccgcggctgg cgatcgtatc gtcttcgtcg caacgcaaag
    19441 tccaccaaca gccgaatccg atcgcggccg cctcttgggc atggccgaaa tcgggcggga
    19501 gcccgtcgat accctcgacg tgttgaggcc cgatgtgcct cgacccgagg actacgacga
    19561 tgccggcagg tttcgctggc cgaagggcct cttgatgatt cgcgcatggg ccttcgatcc
    19621 ttcgcctctg ctgcgcgacg tgctggcggc acagcttccg tacaacgcca cgccccaggc
    19681 cgtcatgctc tcggagaggg acgcggcggc cgtgctctcg ttgaccagga aggagatcgc
    19741 aataccagaa agtccggcac aggaacgagc ccggttgctg gatgcagcac tccgggcgaa
    19801 ccgtccgacg accggaccgc ggcctgtcgc ctggcaaggc gagacaaaca gaaccaccga
    19861 tcaacctgcc gtcacttacg ccctgcgttt cggcgacagc gatatctgga agatcggcca
    19921 cgcggtcgac ctcgaaaagc gggtcaagga gatcaactgg catatcccca atgagatcat
    19981 tgctgatgcc tggggtcccg cccttcagca gaaatggccg aaccaggaat ccgcttttgc
    20041 catggagcaa cgggttctcg gtcttctgtc cgaaagaaga acgagagccg agcgcctgcg
    20101 ctgcccgaag tccgaactgg agcgagcctg gacaagagcc atcggcatcg gcatctgact
    20161 ggcgcggtcg cagtccttgg acggcatgat cttccgcctg ccctgatcag tccttgccgg
    20221 agccaccttc ggccagatgc tcgtcgcggc cgatgtcgcg gcggccgacg gcgatcgact
    20281 tgagcacggc atggacgggc cggccgggct cgagcgccag gacgcgcagc gagcggcggg
    20341 tgatccgggc gaggatgaaa tcgtcgccag agaccagctt gacgtcgacg atcggctggt
    20401 cgagcgcgcc gatctcgacg accgtggccg gcaggcagtt gagggcgctg agcccgaccg
    20461 gcggtgccgc ggccaggatg acgtcgcggg cgcggatgcg cacccgaagc tgcgtgccga
    20521 tcggtgcctc gaccagcggc acccagagcc ggccgccagt gacctcgagc tcggtcagct
    20581 cgtcgatcgg gtcgtgcgcc aggatgcggg cggcgaggag ggcgccgccc tcgtgccggc
    20641 ccatgagcgg gaagaggtcg gggtcggcca tcagcgcggc cggtgggccg caccgctcga
    20701 cgcggccatc ggcgaggacg acgaccgtgg tggcgaggcg cgcgatctcc ggcaccgagt
    20761 ggctgacata gaggatcggc aggccgaacg cgtccctgag ccgctcgata tagggcagga
    20821 tctcggcctt gcgcgcctcg tcgagcgagg cgagcggctc gtccatgagg aggagcctgg
    20881 ggctcgcgag gagggcccgg ccgatcgcca cgcgctgctt ctcgccgccc gagagactgc
    20941 ccggacggcg ctccaggagg tgaccgatgc cgagcagctc gaccgtgtcg gcgaagctgc
    21001 cgaagcggtg ttggtgacgc tggaaccagc ggccgaagcc gagattctgg cgcacgctga
    21061 gatgcgggaa gagccggcct tcctggaaga catagccgat gcgccggcgg tgcggcggca
    21121 ccacgatgcc ccgctgggta tcgagcagca cctggccgtc gacggtgacc cggccctgct
    21181 cggggcgcag cagcccggcc acggcgttga ccaccgtcgt cttgccggcg ccggagcggc
    21241 cgaagagcgc ggtgaggccg gcgccggacc tgaaggtggc gtcgatcgtg aagccgccga
    21301 ggcggtggtt gatggcgacg tcgatgctca tcgctgggtc tcgctcatcg gtcggcgatc
    21361 cgccgggcca cgagccgggc gacggcctcc gaggcgagca gcgccgccat ggccacggcg
    21421 atcgagacgg cggtcagccg cagggcgccg gactcgccgc ccggcacctg gatcagcgcg
    21481 tagatcgccg acgggatcgt ctgggtctcg cccgggatgc tcgagacgaa ggtgatggtg
    21541 gcgccgaact cgcccatcgc cttggcgaag gcgagga
//
LOCUS       DAOTFI010000054        21560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2910_length_21560_cov_6.828645, whole genome shotgun sequence.
ACCESSION   DAOTFI010000054 DAOTFI010000000
VERSION     DAOTFI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21560
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2910_length_21560_cov_6.828645"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1633
                     /locus_tag="P5558_07360"
     CDS             299..1633
                     /locus_tag="P5558_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY24162.1"
                     /translation="MHDDFQAKQRRLRSYLMRRRQVLHGGLAGAAAVALPGLSRRALA
                     QADPPLSFVGWQYNPQIVEENVNIFRELYGENVNYELVPGEYHAVVETKLIGGQKIDM
                     MYAEEDRLVRWWRANWVRDIEDLPGVPEIKAGMFDINVHNMTLPDGKLGGLPYYTGFN
                     SFVCNQRHLDAAKLEPPATWPELLEQCRKLKADGVAEYPYMSAWQRAWASLSWSLFSI
                     WYSEGAPVFDANFDPVFDDAFRGVLEMHRTMYDEELVPADIFTLDQEGVPAFATGNHS
                     FMVVHEYDQKVFNDPALSQFAGDCINAVMPGQTRSTFIWTALYLMGKNPVDLERVWNL
                     QQFFGGKAKDGKYHVATRWALEFGLGTPHKEVIESPEVQAAFGKWKNMEVAAIQQETA
                     TTRDVAKTMWFPEWDWYMMGEVQDYIRGAQETDELVDKLHDKAVELKQLYPQ"
     gene            1715..3649
                     /locus_tag="P5558_07365"
     CDS             1715..3649
                     /locus_tag="P5558_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY24163.1"
                     /translation="MSSATIEKAADQRHPTRRSSVESLRLPVLMTLPTLVIVFVVIGI
                     PLVYSLALSLHRINMLTQQWIFVGLQNYLEILPQPDFLYAMGRTLYFAVVTVSGGLAL
                     GMAMALVLNQRFWGRGVLRSFVLIPWAMSPVAVGILWGWMLNGNYGTFNGILTDLGLV
                     EKPIAWLANGWAALNLVALVHIWNQAPLASLLILAGLQSMPENLHRAARIDGAGPLVR
                     FFRITLPWLRPMLLLVLILTTINSIMAFDLFWTITKGGPGSATTVFSWMGYAYAFQFF
                     RFGEGAAILYVLTILCLGLAWLYLRLFFPRTRRPPAGSDVEAKPRGLGHELVLRVESR
                     VQAARARLTGRVLGAGWPGARLRRLLFRLGLGTGVLLIFAWSAGPFLWLVIMSLSPSV
                     DLVRTPPTVIPETLTLENFRYVLFPGGAVDGQSSVQATRVPLSIWNSFVVASFVTAIN
                     VVIGSLAGYAYARHGRGKFMTGSLWALMMTRMTPSLALILPFFIIFRMADLIDTRTAL
                     VIAYCSLILPLSTWIMKGYFEGLPPNLERAALVDGCTRLEAIIKIILPVAKPGLVAAG
                     IFCFLVSWNEFIFALILTSTPQSQTIPVIISGFLVQLRFYDYGPMFAASVLAILPPVL
                     LALVFQRHLVSGMLSGSLKG"
     gene            3659..4753
                     /locus_tag="P5558_07370"
     CDS             3659..4753
                     /locus_tag="P5558_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24164.1"
                     /translation="MARVTIDRMSKDYGAVRAVNGLALEIADGELLTLLGPSGCGKST
                     ALSCIAGLERLSEGTIRFDDRDVTELEPHQRNVAMVFQDYALYPHMDVRDNISFGLKQ
                     QKVDPAAITQQVEKAAGMLDLGSYLDRRPAELSGGQRQRVAVGRAVVRDPAVLLMDEP
                     LSNLDAGLRLRTRTEIKRLQRELGVTTVFVTHDQEEAMVLSDRMAVMRDGLLQQIGPP
                     MEVYREPVNLFVASFIGSPAMNFLDVELVREGNTLLCRFAERTLRLALGSVSEASLKS
                     LDRPKKALLGIRPSDLLVGGSAREQTIEGRVFLVEPVGPVTFLDVDVEGRVVKAACDP
                     DTAPVVGEVVPLGFADSRVRLFDQATEQRL"
     gene            4793..5812
                     /locus_tag="P5558_07375"
     CDS             4793..5812
                     /locus_tag="P5558_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY24165.1"
                     /translation="MRRVKMAVIGAGTWGENHAMAFSTYPIVDLQYICDLDGDRARRL
                     AEKVGCGWTDRIEEIASSDVEAVSIATPDHAHKGPCLAMIEAGKHILVEKPFTIDLAE
                     GHEIIAAAEAKGVKLMVDFQLRWHPHYMGAKHFMESGELGEGVMGYARLSDTIHVPTE
                     MLSWGSASGPEWFLFPHTMDVTRWILDEEPVEVFARGSRRVLKGMGIDAYDAIQALFQ
                     FERSFVTFETAWIIPDSYPTVVDNKFTLHGSKGRIELDSTPGIAISSDRYKYPFSSHA
                     ITRYGKPFAHFYESIRHFADCVLEDKEPAATGHDGLMATAMIQATLRSLEAGRPVRMD
                     EVLAA"
     gene            5809..6294
                     /locus_tag="P5558_07380"
     CDS             5809..6294
                     /locus_tag="P5558_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24166.1"
                     /translation="MSGPRERARLVEALVAVLLPGDGDFPSGAEAGVQGKLAERLLET
                     SGAAGLERVITGLAGLAEAGEAGPDMVRAFERAEPALFGAVRKIAYLTYYESPFVQEA
                     IRALGFAYNAAPLPKGYGVGRFDEATDRPTHGRGRYVKTNEVRRVDLSGLDLEELRHG
                     H"
     gene            6284..7918
                     /locus_tag="P5558_07385"
     CDS             6284..7918
                     /locus_tag="P5558_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRY24167.1"
                     /translation="MATEAKGGHSDVLVIGAGATGAITSLILAGAGLDVVCLEQGGWT
                     AAEDHPHFSRDWEWQKQGRWNPDTNVRQAASDYPVETNSSNVLMWNAVGGSTNVYGAL
                     WPRYRPSDFRKGVEHGLAPDWPITYEDLADPYDRVDRLIGVSGLAGNPALPPHGPYPT
                     GPLPLRKSGRALASAFDRLGWHWWTLPAGVIAEDYDGRPACNGCGACTAGCARNSMNM
                     FSLSIWPKALAAGVELRIQAAVERVEKGQDGRATGAVYIDRASGRRVRQTADVVVLAC
                     NGVGTPWLLQASDNLANSSDQVGRNLLHHTLTSVDIWGEDIVDTHMGYTGSLISMEFA
                     ETDTSRGFVNGFNFNCAATGHAGSQSLGMLARSPAPWGAAHHEWFRSRFDRGFGVFAI
                     GDDLPSPDNRITLSASLKDELGRPAPKLRYVPGDNDERMMRYQLERLEEIAKAAGAVD
                     YRLNDYKDADGVYRTPAWHLLGTCRMGADPESSVVNKWHQSWDVPNLFIVDGSVLATG
                     GVVNPTPTVTALALRAAEHLRDNFAELRRATKPLEA"
     gene            8440..9459
                     /locus_tag="P5558_07390"
     CDS             8440..9459
                     /locus_tag="P5558_07390"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRY24168.1"
                     /translation="MTRDELLARLDAIPRVPLAHLPTPLEPAPRLTAALGGPEIWLKR
                     DDLTGLAFGGNKTRQLEYVFADILAKGADTVIGSAYTQSNWCRQMTAAARKLGLDIHL
                     VLLHGEKGPALQGNFLLYRLMGAEIEVVDLTSLEQVQPLLEAKAAKLREAGRKPYIVG
                     PMQTENLVTGALGYVRAALEIDEQLERQGFAPSHLYLSGANMTPAGLALGFELIGRKL
                     ALHSIAPIKWETDRAWDIARIATATARRLGLDATIDPAAILSSDDYIGPRYGVVSDAG
                     REALALVARTEGVILDPVYSSKAMAGLIDHVRKGRLTAQDKVVFVHTGGTPALFAYAE
                     DVIVD"
     gene            complement(9463..11262)
                     /locus_tag="P5558_07395"
     CDS             complement(9463..11262)
                     /locus_tag="P5558_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRY24169.1"
                     /translation="MTRAAAPLPDTVERMKSRDFRQLRRLAAYLAPYRWRVAGALVAL
                     VVAAAAVLSLGVGLRYLIDSGFGAGRPQALDHALRASLLVILALAVATYLRSYLVTWL
                     GERVVAELRNQVYRHIIRMPPGFFETTRTGEVLSRLTTDTTVVQTVIGSTVTQALRNI
                     LLTVGGIGLLVYSNPKLSGLVLLVVPLVVVPIVVFGRKVRRLSRTAQDRLADVSGRAE
                     ETVNAVRTVQAFAQEEREATTFAAASEAAFQAAAARAKARSMLAAVVITLVFGAIVAV
                     LWMGGQDVLAGRITAGELSAFVFFATVVASAVGGLSDIVGDLQRAAGATERLFELLDS
                     EPAIRAPAAPLALPERTAGEVRFEAVRFAYPGHPERIILDGLDLAVRPGESVALVGPS
                     GAGKTSIFQLLMRFYDPQAGRIRLDGQPIDQLDPARYRSRLGLVPQEPVIFSADAWTN
                     IRYGRPEASDAEVLEAAETAAARGFIEALPDGFATFLGEKGVRLSGGQRQRIAIARAI
                     LRDPAVLLLDEATSSLDAESEFAVQQALDYLMRNRTSIVIAHRLATVLKADRILVMED
                     GQIVDAGTHRELIGRGGLYARLADLQFRQGEAA"
     gene            11408..11632
                     /gene="rpmE"
                     /locus_tag="P5558_07400"
     CDS             11408..11632
                     /gene="rpmE"
                     /locus_tag="P5558_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRY24170.1"
                     /translation="MKKDIHPAYHEINVVMTDGSTFKTRSTWGAEGDTLTLDVDPKSH
                     PAWTGGSMQLRERGQVEKFNRRFQSFRRKA"
     gene            11663..11788
                     /gene="ykgO"
                     /locus_tag="P5558_07405"
     CDS             11663..11788
                     /gene="ykgO"
                     /locus_tag="P5558_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRY24171.1"
                     /translation="MKVRNSLKTARTRHKDCRLVRRKGRVYIINKKDPRFKARQG"
     gene            11860..13125
                     /locus_tag="P5558_07410"
     CDS             11860..13125
                     /locus_tag="P5558_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRY24172.1"
                     /translation="MGRAGLDKLLRAMLDAAVAVAMPDRAVPAHLPMPPKGRTIVIGA
                     GKASAAMARAVEQHWPGPLEGLVVTRYGHAVPCEHIEIVEAAHPVPDAAGEAAAARIL
                     AMVEGLTEDDLVLALISGGGSSLLALPAPGLGLEDKQAVNRALLRSGASIDEMNCVRK
                     HLSAIKGGRLAAAAHPARLVALMISDVPGDDPAVIASGPTVPDPTTRADALALVEKYG
                     IELAPHVRRHLEDPACESVKPGDPRLERAETVMIATPQASLEAAAEVARAAGITPLIL
                     GDSIEGESRDVAKVMAAIAKQVIRHGQPCPRPCVLLSGGETTVTVRGDGSGGRNVEFL
                     LALAVALDGLAGVSAIAADTDGVDGAAEVAGAWIDDATLAKAASLGLVAKASLARNDG
                     HTFFGTLGQQVVTGPTLTNVNDFRAVLID"
     gene            13266..14375
                     /locus_tag="P5558_07415"
     CDS             13266..14375
                     /locus_tag="P5558_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY24173.1"
                     /translation="MSTVRQILLSLLLLGGAVGGYYFYHHLTAASVIPGDGKGGGRTI
                     SVEVARAVRETVRDRAEAVGTTLANQAIEVVSLASGRITELLFEPGQAVEAGAVLARL
                     DDAIEEADLDEARAMAKEAQSALDRARRLRASNNIAEATVVELEAAAAAAQARVDRAA
                     QRLEDRLVRAPFDGVVGIRRVDLGARVDNDTVITTLDDRSRMIVEFMLPEFFYGRIQP
                     GLEVQASSTSFPDRSFAGTLTSIDTRVDVTSRAFRVRASLPNPDLLLPAGLFVLVDIT
                     LGERDAVIVPEEAVVSEADRSFVYVAGPERAERRLVELGARSLGVVEIENGLEAGELV
                     VTSGLQRLRDGVAISYEAPKAIDPERIALPSGDIG"
     gene            14372..17488
                     /locus_tag="P5558_07420"
     CDS             14372..17488
                     /locus_tag="P5558_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY24174.1"
                     /translation="MTLSELSIRRPVLATVVSLLIVVFGIAALSRLPVRELPDVDRAV
                     VSITTSYTGAAPEIVDTDITEVIEGAVAGVSGVREITSESRRGRGRTTITFNGDRDID
                     EAANDVRDAVARVRARLPDDADEPQIVKSDSDADPVMRLGIVSDRMSPAEITDYVERF
                     LVDRLATLDGVANVEINGQRRFAIRIWLDRRALAARDLTTADVEAALRRSNLELPAGE
                     VESTARELTVRLDSRLRDVESFRRLVIATAGGYPIRLADVARVELGAEDDSTFVRSDG
                     RPAVGINVLRQSQANTIAISDAVRAEIELLRPILPAGMDIIVGSDDAIFIAASIREVL
                     TALGISIILVVLVIFVFLLSLRATLVPAVTIPVAVVGSFILIQALGFSINVLTLLALI
                     LAIGLVVDDAIVMLENIQRRIEAGEKPITASVLGARQVTFAIIATSLTLIAVFVPLSF
                     MQGQVGRLFIEFGLVLAAAVLISTFVALTLCPALCSRVLKGRSAGAAGTTGGGRAARA
                     VGNVYRRLLLRALAAPLPVLVMGLLLAATSYGLYVALPQELTPIEDRGVFFVPVIAPQ
                     GSTVTYTDERVAEVEGVIRPLLEDGEVERLFAIAGSFNSNNRGFVVGRLADWGLRDRS
                     QQAIINELAPRISGITGARAFPVSPAGLGLGGSSSPLRVVIGGPDFQSAKEWADAIVE
                     RAEANPQLLNLDVDFEENQPQLTLRVDRDRADDLGISLETIAQTLQTLLASREATEFI
                     DRGREYPVILQARAEDRRSPTDLENIFVRGTRSGTLVPLTALVLAEEGASAPALRRYD
                     RLPAVAITAALADGYDLGSAIAYIEAIALETLPEEARLGFAGQSREFIEARGGMLFTF
                     AMALLIVFLVLAAQFESWIHPLIIMLTVPLAVAGALLALYVTGLSLNVYSQIGIVLLV
                     GLMAKNGILIVEFANQLRDEGHGVREAVVEAAVLRLRPIVMTVVSTVLGAVPLVIATG
                     AGAESRIAIGTVIIGGLGLASVLTLVLTPVLYDLLARFTRPASAIGRELEAELSAGLN
                     GRRTTRAG"
     gene            complement(17457..17786)
                     /locus_tag="P5558_07425"
     CDS             complement(17457..17786)
                     /locus_tag="P5558_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRY24175.1"
                     /translation="MVYLQLVVAIACEVAGTTALQASQQFTRFWPSVAVVVCYGLAFF
                     FLALVLRTMPVGIAYAIWSGLGIVLISLVGLVVFGQRLDLWAIVGLGLIVAGVLIVNL
                     LSSSVGH"
     gene            17838..18434
                     /locus_tag="P5558_07430"
     CDS             17838..18434
                     /locus_tag="P5558_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY24176.1"
                     /translation="MTVIYYLSHPEVTIDPAIPVPGWGLSATGRARVLAVRDAAWLRA
                     CRRIVTSAERKAVETAELVAETSAAEIVVRPAMHENDRSATGFLVPAEFEQVASEFFA
                     RPEESVRGWERASDAQARIAGELEAVLAGHDPTLPLLLVGHGGVGTLLYCRHAGLAID
                     RRHDQPRSGSVFALDARSLAPLFTWRPMEEVERVLGPC"
     gene            18489..19691
                     /locus_tag="P5558_07435"
     CDS             18489..19691
                     /locus_tag="P5558_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HRY24177.1"
                     /translation="MARAYVCGTLDSKATELGFIQACLEAAGVATCLVDLGTSGEAAP
                     ADVAAREVAAHHPKGEAAVFTGDRGSAVGAMAEAFARFVLTRDDIGGMIGAGGSGNTA
                     LVTPAMRALPVGVPKVMVSTVASGNVAPYVGPADILMLHAVADVQGLNAITRRVLGNA
                     AHALAGMMLHPIPAPAAATKPAIGLTMFGVTTSCVQQVTRALEADYDCLVFHATGTGG
                     QALEKLVASGLVGAMLDITTTEIADLLVGGVLPATEDRLEAVIQTGIPYVGSLGALDM
                     VNFGAMETVPERFRDRRLYVHNPQVTLMRTTVEENARFGAWLAARLNRMEGPVRFLVP
                     EGGVSAIDAPGQPFFDPDADAALFAALEADFRPAPNRRLARVAHNINDAAFAAACVAA
                     FHEITRTS"
     gene            19692..20528
                     /locus_tag="P5558_07440"
     CDS             19692..20528
                     /locus_tag="P5558_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HRY24178.1"
                     /translation="MPRFERQALVEKFQDMVRRRVPIIGGGAGTGLSAKCEEAGGIDL
                     IVIYNSGRYRMAGRGSLAGLMAYGNANDIVVEMAREVLPVVKKTPVLAGVNGTDPFML
                     RDRFLRDLKDMGFAGIQNFPTVGLFDGVMRTNLEETGMGYGLEVDLVRAAHELDMLTT
                     PYVFSADEAVAMTEAGADLIVPHMGLTTGGTIGAETAVKLEDCPRIIDEWAAAARRVR
                     SDVIVICHGGPIATPEDAAFVLARTEHCHGFYGASSMERLPTEKALTAQVAAFKEIGF
                     GA"
     gene            20525..20911
                     /gene="dhaM"
                     /locus_tag="P5558_07445"
     CDS             20525..20911
                     /gene="dhaM"
                     /locus_tag="P5558_07445"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HRY24179.1"
                     /translation="MSATVGILVVSHSPKVAEGAVDMVRQMVGDEVACTGVGGDPAGG
                     LGTDAAKILAGIESVWSEAGVALLVDLGGAEMNSEMAILMLDEARRGRVRIAAAPVVE
                     GAVIAATEASAGSTLDEVVAAAEEWR"
     gene            20911..21192
                     /locus_tag="P5558_07450"
     CDS             20911..21192
                     /locus_tag="P5558_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRY24180.1"
                     /translation="MAAERARVRVNHPTGLHARPAVKLTRLAKGFEASIRLRGLPDGA
                     WIDAKSIVKVMALKLKTGTLIELEAEGADAGAALEGLTGLVERNFDEHG"
     gene            21185..>21560
                     /locus_tag="P5558_07455"
     CDS             21185..>21560
                     /locus_tag="P5558_07455"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRY24181.1"
                     /translation="MAEKHYKGRVAAPGLALGPLFHLPDLAVAADRPAGTPEAEMARL
                     TAAIAAAREQLALLLAEGEEMAAEILGFQLEMLDDPALVETAAALVAEGAPAVRAWQE
                     GLAPQVEVFAGDDDLYFRARAAD"
BASE COUNT         3306 a         7438 c         7490 g         3326 t
ORIGIN      
        1 ccggaatgtc gtcgtcgagg gcggcgaccc gccccttggc gaaggcgaca tcggcgcgcc
       61 gatcgacgtt ctggcccgga tcgaggacgt ggccaccttt gaggatcaga tcgaacaagg
      121 acgaggctcc cgcgggcgcc cactcaccgc gtggccgcgc acatttttct gtttccaata
      181 agcatgacct gtttacgata cgaaacagca agagaaaaca ggtttcgcag cggtcgcagc
      241 catcgcgatc gctgtgccag caggcgtgaa acgcaagaaa ccggggaagg aaagcggcat
      301 gcacgacgat ttccaggcga agcagcgacg gctgaggtcc tatctgatgc ggcgccgcca
      361 ggtgctccac ggcggtctcg ccggagccgc ggcagtggcg ttgcccggcc tctcgcgccg
      421 ggcgctggcc caggccgatc cacccctgag ctttgtcggc tggcagtaca acccgcagat
      481 cgtcgaggag aacgtcaaca tcttccgtga gctctatggc gagaacgtca actacgagct
      541 cgtgcccggc gagtatcacg cggtcgtcga gaccaagctg atcggcggcc agaagatcga
      601 catgatgtat gccgaggagg accggctggt gcgctggtgg cgcgccaact gggtgcgcga
      661 catcgaggac ctgccgggcg tgcctgagat caaggccggc atgttcgaca tcaatgtcca
      721 caatatgacc ctgccggacg gcaagctcgg tggcttgccc tactacaccg gctttaattc
      781 cttcgtctgc aaccagcggc acctcgacgc cgcgaagctt gagccgccgg cgacttggcc
      841 cgagctcctc gagcaatgcc gcaagctcaa ggcggatggt gtcgccgaat atccctacat
      901 gagcgcctgg caacgggcct gggcgagcct gagctggagt ctcttctcga tctggtactc
      961 cgagggcgcg ccggtcttcg acgccaactt cgatccggtg ttcgacgatg ccttccgcgg
     1021 cgtcctggag atgcatcgga cgatgtacga tgaggagctg gtgccggccg acattttcac
     1081 cctcgatcag gagggtgtgc cggccttcgc caccggcaac cacagcttca tggtagtgca
     1141 cgagtacgac cagaaggtgt tcaacgaccc ggcgctgtcg cagttcgccg gcgactgcat
     1201 caacgccgtc atgccgggcc agacccgctc gaccttcatc tggacggcgc tctacctgat
     1261 gggcaagaac ccggtcgatc tcgagcgggt ctggaacctc cagcagttct tcggcggcaa
     1321 ggcgaaggac ggcaagtacc atgttgccac ccgctgggcc ctcgagttcg gcctcggcac
     1381 gccgcacaag gaggtgatcg aatcgcccga ggtgcaggcc gccttcggca agtggaagaa
     1441 catggaggtg gcggccatcc agcaggagac ggcgacgact cgcgatgtcg ccaagaccat
     1501 gtggttcccg gagtgggact ggtacatgat gggcgaggtg caggactaca tccggggtgc
     1561 ccaggagacc gacgagctgg tcgacaagct gcatgacaag gcagtcgagc tgaagcagct
     1621 ctacccgcaa tagtcgccga ccgcggcccc gtccgaagcc cgggcggggc cggcgagctc
     1681 tcagccagtt cgaagccctc ttccgcgagc gcccatgagc tcagccacga tcgagaaggc
     1741 cgccgaccag cggcacccaa cgcggcggag cagcgtcgaa tcgctgcgcc tgccggtcct
     1801 gatgacgctg ccaacgctgg tcatcgtctt cgtcgtcatc ggcattccgc tggtctacag
     1861 cttggcgctc agcctgcatc gcatcaacat gctgacccag caatggatct tcgtcgggct
     1921 gcagaactac ctcgagatcc tgccccagcc ggatttcctc tacgccatgg ggcgcacgct
     1981 ctatttcgcg gtggtcaccg tcagcggcgg cctggcgctc ggcatggcca tggcgctggt
     2041 gctcaaccag cgcttctggg gccgcggcgt cctgcgcagc ttcgtgctga ttccctgggc
     2101 catgtcgccg gtcgccgtcg gcatcctgtg gggctggatg ctgaacggca attacggcac
     2161 gttcaacggc atcctcaccg acctcggcct cgtcgaaaag ccgatcgcct ggctagccaa
     2221 cggctgggcg gcgctcaatc tcgtggccct cgtccacatc tggaaccagg cgcccctggc
     2281 ctcgctgctg atactcgctg gcctgcagtc gatgccggag aacctccacc gtgccgcccg
     2341 gatcgatggt gccggaccct tggtgcgctt tttcaggatc actctgccct ggctgcggcc
     2401 gatgctgctt ttggttctga tcctgacgac catcaactcg atcatggcgt tcgatctctt
     2461 ctggaccatc accaagggcg ggccgggcag cgccaccacg gtgttctcgt ggatgggcta
     2521 cgcctacgcc ttccagttct tccgcttcgg cgagggggcg gccatcctct acgtgctgac
     2581 catcctctgc ctggggctcg cctggcttta tctcaggctc ttctttccga ggacccggcg
     2641 tccgcctgca gggtcagatg tcgaggcgaa gccgcggggg ctcggccacg agctggtcct
     2701 gcgggtcgaa agccgggtcc aggcggcgcg ggcgcggctc accgggcgtg tgctgggcgc
     2761 cggctggccc ggcgcccggc tgcgcaggct gctcttccgg ctcgggctcg gcacgggcgt
     2821 cctcctgatc ttcgcctggt cggccggacc gtttctctgg ctcgtcatca tgagcctgtc
     2881 gccctccgtc gacctggtgc gcacgccgcc gaccgtgatt ccagagacgc tgaccctcga
     2941 gaacttccgc tacgtgctct tccccggcgg cgccgtcgat ggccagtcga gcgtgcaggc
     3001 gacacgcgtg ccgctctcga tctggaacag cttcgtcgtc gcctcattcg tgaccgccat
     3061 caatgtcgtg attggctcgc tcgccggcta tgcctatgcg cggcacggcc ggggcaagtt
     3121 catgaccggc tcgctctggg ccttgatgat gacgcggatg acgccgagcc tggcgcttat
     3181 cctgcccttc ttcatcatct ttcgcatggc cgacctgatc gacacccgaa ccgcgctggt
     3241 gatcgcctat tgcagcctga tcctgccgct ctcgacctgg atcatgaagg gctacttcga
     3301 gggcctgccg cccaatctcg agcgggccgc cctggtcgac ggctgcacgc gccttgaggc
     3361 catcatcaag atcatcctgc cggtggcgaa gccgggcctg gtggcggccg gcatcttctg
     3421 cttcctggtg agctggaacg agttcatctt cgcgctgatc ctgaccagca cgccgcaatc
     3481 gcagaccatc ccggtgatca tctcgggctt cctcgtccag cttcgcttct acgactacgg
     3541 gccgatgttc gcggccagcg tgctggcgat cctgccaccc gtgctgctgg ccctcgtctt
     3601 ccagcgccat ctggtgagcg gcatgctctc cggctcactc aaaggttagg agtatgccgt
     3661 ggccagggtg accatcgacc gcatgagcaa ggactacggc gcggtccgtg ccgtgaacgg
     3721 gctggcgctc gagatcgccg atggcgagct tctgaccctg ctcggaccgt cgggctgcgg
     3781 caagagcacc gcactctcct gcatcgccgg gctcgagcga ctgtccgagg ggaccatccg
     3841 cttcgacgat cgggacgtca ccgagctcga gccgcaccag cgcaatgtgg ccatggtctt
     3901 ccaggactac gcgctctacc cgcacatgga cgtgcgcgac aatatcagct tcggcctcaa
     3961 gcagcagaag gtcgatccgg ccgcgatcac gcagcaggtc gagaaggcgg ccggcatgct
     4021 cgatctcggc tcctacctcg accgccggcc ggccgagctc agcggcggcc agcgccagcg
     4081 cgtggccgtg gggcgggcgg tcgttcgcga tccggcggtg ctcttgatgg acgagccgct
     4141 ctcgaatctg gacgccgggc tgcgcctgcg cacccggacc gagatcaagc ggctgcagcg
     4201 cgagctcggt gtcaccacgg tcttcgtcac ccatgaccag gaggaggcga tggtgctctc
     4261 cgaccggatg gcggtgatgc gcgacgggct cctgcagcag atcggcccgc cgatggaggt
     4321 ctatcgcgag ccggtcaatc tcttcgtcgc ctccttcatt ggctcgccgg cgatgaactt
     4381 cctcgatgtc gagctggtgc gcgaaggcaa cacactcctc tgccgcttcg ccgagcggac
     4441 gctgcggctg gcgctgggga gcgtaagcga ggcgtcgctg aagagcctcg accggccgaa
     4501 aaaggcgctc ttgggcatcc gcccgagcga tcttctggtc gggggcagcg cgcgcgagca
     4561 gacgatcgag ggacgggtct tcctggtgga gccggtgggg cctgtcacgt tcctcgacgt
     4621 cgacgtcgag ggccgggtgg tcaaggcggc ctgcgacccg gacacggcac cggtggtcgg
     4681 cgaggtggtg cccctcggct tcgccgacag ccgcgttcgc ctcttcgatc aggcgacgga
     4741 gcagcgactc tagacgaggg tggcggacgc cacatcagcg aaggaaaaga cgatgcgcag
     4801 ggtcaagatg gcggttatcg gcgccggcac atggggcgag aaccatgcca tggccttctc
     4861 gacctatccg atcgtggatc ttcaatacat ctgcgatctc gacggggatc gggcgaggcg
     4921 gctggcggaa aaggtcggct gcggctggac cgaccggatc gaggagatcg cgtcgagcga
     4981 tgtcgaggcg gtgagcatcg ccacgccgga ccatgcccac aagggcccct gcctcgccat
     5041 gatcgaggcc ggcaagcaca tcctggtcga gaagccgttc accatcgatc tcgccgaggg
     5101 ccacgagatc atcgccgccg ccgaggccaa gggtgtcaag ctcatggtcg atttccagct
     5161 acgctggcac ccgcattaca tgggtgcgaa gcacttcatg gagtcgggcg agctcggcga
     5221 gggtgtcatg ggctacgccc gcctctccga cacgatccat gtgccgaccg agatgctgag
     5281 ctggggcagc gcgtccgggc ccgaatggtt cctctttccc cataccatgg acgtcacccg
     5341 ctggatcctc gacgaggagc cggtcgaggt cttcgcccgc ggcagcaggc gcgtgctcaa
     5401 gggcatgggc atcgacgcct atgacgcgat ccaggcattg ttccagttcg agcgcagctt
     5461 cgtcaccttc gagaccgcct ggatcatccc ggatagctat cccaccgtgg tcgacaacaa
     5521 gttcacgctg cacggcagca agggccggat cgagctcgac agcacgcccg ggatcgccat
     5581 ctccagcgac cgctacaaat atcccttctc gagccatgcc atcacccgct acggcaagcc
     5641 cttcgcccat ttctacgaat cgatccgcca cttcgccgac tgcgtgctgg aggacaagga
     5701 gcccgcggcc accggccatg acgggctgat ggcgaccgcg atgatccagg cgaccctgcg
     5761 ctcgctcgag gcagggcggc cggtgcgcat ggacgaggtc ctggctgcat gagcgggcca
     5821 cgcgaacgcg ccaggctcgt cgaggcgctg gtggctgtgc tcctgccggg cgacggcgat
     5881 tttcccagtg gtgccgaggc cggcgtgcag ggcaagctcg cggagcggct actcgagacc
     5941 tcgggtgccg ccgggctgga gcgggtgatc accggtctcg ccgggctggc cgaggcgggc
     6001 gaggccggcc ccgatatggt gcgagccttc gagcgggcgg agcccgcgct cttcggtgcc
     6061 gtgcgcaaga tcgcctacct cacctactac gagagcccgt tcgtccagga ggcgatccgg
     6121 gctctcggct tcgcctataa tgcggcacct ctgcccaagg gctacggggt gggtcgcttc
     6181 gacgaagcaa ccgaccggcc gacccatggc cggggtcgtt atgtgaagac aaatgaggtg
     6241 cggcgcgtcg atttgagcgg tctcgatctc gaggagctgc ggcatggcca ctgaggcaaa
     6301 aggcgggcac agcgacgtcc tcgtcatcgg cgccggtgcc accggtgcga tcaccagcct
     6361 gatcctcgcc ggggccgggc tcgacgtcgt ctgcctcgag cagggcggct ggacggcggc
     6421 cgaggaccat cctcatttca gccgcgactg ggaatggcag aagcaggggc gctggaaccc
     6481 cgacaccaat gtccgccaag cggcgagcga ctacccggtc gagaccaact cctccaacgt
     6541 cctcatgtgg aacgcggtcg gcggctcgac caatgtctac ggtgccctct ggccgcgcta
     6601 ccggccgtcg gacttcagga agggcgtcga gcacgggctc gccccggact ggccgatcac
     6661 ctacgaggac ctggccgacc cctatgaccg ggtggatcgg ctgatcgggg tgagcgggct
     6721 cgccggcaac ccggccttgc cgccgcatgg cccctacccc accgggcccc tgccgctgcg
     6781 caagtcgggg cgggcgctgg ccagcgcctt cgaccggctc ggctggcact ggtggacgtt
     6841 gccggccggc gtgatcgccg aggactatga cggccgcccg gcctgcaacg gctgcggcgc
     6901 ctgcaccgcc ggctgcgccc gcaactcgat gaacatgttc tccctttcca tctggcccaa
     6961 ggcgctggcc gccggggtcg agctgcgcat ccaggcggcg gtcgagcggg tcgagaaagg
     7021 ccaggacggg cgggccacgg gggcggtcta tatcgaccgg gcatccggcc ggcgggtgcg
     7081 ccagacggcc gatgtcgtgg tcctcgcctg caacggcgtc ggcacgccct ggctgctgca
     7141 ggcctccgac aatctggcca acagctcgga ccaggtgggg cgcaacctcc tgcaccacac
     7201 gctgacctcg gtcgacatct ggggcgagga catcgtcgac acgcatatgg gctataccgg
     7261 ctccctgatc agcatggagt tcgccgaaac cgacacgagc cgcggcttcg tcaacggctt
     7321 caacttcaac tgcgccgcca ccggccatgc cggcagccag tcgctcggca tgctggcccg
     7381 gagcccggca ccctggggtg ccgcgcatca cgagtggttc agaagccgct tcgaccgggg
     7441 cttcggcgtc ttcgccatcg gcgacgacct gccctcgccc gacaaccgga tcacgctctc
     7501 ggcatcgctg aaggatgagc tcggccggcc ggcgccgaag ctgcgctacg tgcccggcga
     7561 caacgacgag cggatgatgc gctaccagct cgagcggctc gaggagatcg ccaaggccgc
     7621 cggtgcggtc gactaccggc tcaacgacta caaggacgcc gacggcgtct accgcacccc
     7681 ggcctggcac ctgctcggca cctgccgcat gggtgccgat cccgagagct cggtcgtcaa
     7741 caagtggcac cagagctggg acgtgcccaa tctcttcatc gtcgacggca gcgtcctcgc
     7801 gaccggcggg gtggtcaacc cgacgccgac cgtgaccgcg ctggcgctgc gcgccgccga
     7861 gcatctgcgc gacaacttcg ccgagctgcg ccgggcgacg aagccgctgg aggcgtgaca
     7921 taggttttat agcctatttc gatccgggcc gcaagcccgt gcggggttcg atcggcaaat
     7981 catggccctc gggttcgttg ctgcggcggg cggcgccagg atgcacggaa tcgctgtccc
     8041 tgacctcggg ttcgttcgtc gaatccccgg gttccctcgt cgaatccccg ggttcgctcg
     8101 tcgaatcggt gggttcgctc gtcgaatcgg tgggttcgat cgtcggttcc gcgggttcgt
     8161 tcgccggcac ctgtcgcgga atccgcgaca tttcgcgccc gatgtcgcag attctgcgac
     8221 atcacgatcg cttatgtcgc agctttcgcg acatccttgg gttcgctgtc gcggattctg
     8281 cgacaacccg agccgaattg ctgcatgaaa tgcgacgatc cgggctgccg agcgccgatt
     8341 cgccgccgcc acgccccgcc tgccgcgact gccataagct ttcgtgatac tctggcggat
     8401 tcgaagcatc atgccacccg ccccctggag gcccgaaacg tgacccgcga cgaactgctt
     8461 gcccgcctcg acgccattcc gcgcgtgcca ctggcgcatt tgcccacacc gctcgagccg
     8521 gcgccacggc tgaccgcagc gctgggcggg ccggagatct ggctgaagcg ggacgacctg
     8581 accggcctcg ccttcggcgg caacaagacc cgccagctcg agtacgtctt cgccgacatc
     8641 ctggccaagg gtgccgacac ggtgatcggc agcgcctaca cgcagtcgaa ctggtgccgg
     8701 cagatgacgg cggcggcgag gaagctcggc ctcgacatcc atctcgtgct gctgcacggc
     8761 gagaaggggc cggcgttgca gggcaacttc ctgctctacc ggctgatggg ggccgagatc
     8821 gaggtggtcg atctgaccag cctcgagcag gtccagccgc tgctcgaggc gaaggcggcg
     8881 aagctgcgcg aggccggccg caagccctac atcgtggggc cgatgcagac cgagaacctc
     8941 gtcacgggtg cgctgggata tgtgcgggcc gctctcgaga tcgacgagca gctcgaacgg
     9001 cagggcttcg cgccgagcca tctctatctc tcgggtgcga acatgacgcc ggccgggctc
     9061 gccctcggct tcgagctcat cggccgcaag ctggcgctcc acagcatcgc tcccatcaag
     9121 tgggaaacgg accgcgcctg ggacatcgcc cggatcgcca cggcgacggc ccgccgcctc
     9181 gggctcgatg ccacgatcga tccggccgcg atcctgagct cggacgacta tatcgggccg
     9241 cgctatggcg tggtgtcgga cgcgggacgg gaggcgctgg cgctggtcgc ccggacggag
     9301 ggcgtcatcc tcgacccggt ctacagctcg aaggcgatgg ccgggctgat cgaccacgtc
     9361 cgcaagggcc ggctcaccgc gcaggacaag gtcgtcttcg tccacacggg cggcacgccg
     9421 gcgctcttcg cctatgcgga ggacgtgatc gtcgactgag gctcaggccg cctcgccctg
     9481 gcggaactgc aggtcggcga ggcgggcgta gaggccgccc cggccgatca gctcgcgatg
     9541 cgtgccggcg tcgacgatct gcccgtcctc catgacgagg atccggtcgg ccttgagcac
     9601 ggtggcgagg cggtgggcga tgacgatgct ggtgcggttg cgcatcaggt agtcgagcgc
     9661 ctgctggacg gcgaactcgc tctcggcatc gagcgagctg gtcgcctcgt cgagcaggag
     9721 cacggcggga tcgcgcagga tggcccgggc gatggcgatg cgctggcgct gcccgcccga
     9781 gaggcggaca cccttctcgc cgaggaaggt ggcgaagcca tcgggcagcg cctcgatgaa
     9841 gcccctggcc gccgccgtct cggcggcttc caggacctcc gcgtcgctgg cctcgggccg
     9901 gccgtagcgg atattggtcc aggcatcggc ggagaagatc accggctcct gcggcacgag
     9961 gccgaggcgc gagcggtagc gggccgggtc gagctggtcg atgggctggc cgtcgaggcg
    10021 gatgcgcccc gcctgcggat cgtagaagcg catgaggagc tggaagatgc tcgtcttgcc
    10081 ggcgcccgac gggccgacca gggcgacgct ctcgccgggt cggaccgcca ggtcgaggcc
    10141 gtcgaggatg atgcgctcgg ggtggcccgg ataggcgaag cggaccgctt cgaagcgcac
    10201 ctcgccggcg gttcgctccg gcagcgccag gggtgctgcc ggcgccctga tggccggctc
    10261 gctgtcgagc agctcgaaga gccgctcggt ggcgccggca gcgcgttgca gatcaccgac
    10321 gatgtccgag agcccgccca ccgcgctcgc cacaacggtg gcgaagaaga cgaaggccga
    10381 gagctcgccc gccgtgatcc ggccggcgag cacgtcctgg ccgcccatcc agagcaccgc
    10441 gacgatagcc ccgaagacga gcgtgatgac caccgccgcc agcatcgagc gggccttggc
    10501 gcgggccgcg gcggcctgga acgcggcctc gctggcggcg gcgaacgtcg tcgcctcgcg
    10561 ctcctcctgg gcgaaggcct gcacggtgcg cacggcgttg accgtctcct cggcgcggcc
    10621 gctgacatcg gcgagccggt cctgggcggt gcgcgacagg cggcggacct tgcgaccgaa
    10681 gacgacgatc ggcacgacga ccagcggcac gaccaggagg acgaggccgg agagcttcgg
    10741 gttgctgtag acgagcaggc cgatgccgcc gaccgtgagc aggatgttgc gcagcgcctg
    10801 ggtgacggtc gagccgatca ccgtctgcac gaccgtcgtg tcggtggtga ggcgcgacag
    10861 cacctcgccg gtccgcgtgg tctcgaagaa gccgggcggc atgcggatga tgtggcgata
    10921 gacctggttg cgaagctcgg ccacgacccg ctcgccgagc caggtcacga ggtaggagcg
    10981 cagataggtg gcgacggcca gcgccaggat gacgagcagc gaggcgcgga gcgcatggtc
    11041 cagcgcctgc ggccggccgg cgccgaagcc gctgtcgatc aggtagcgca ggccgacacc
    11101 gagcgagagc accgcggccg cggcgacgac gagggccacg agggcaccgg caacccgcca
    11161 gcgatacggg gcgagatagg cggcgaggcg gcggagctgc ctgaagtcgc ggctcttcat
    11221 gcgttcgaca gtgtcgggaa gcggggcggc tgcccgggtc atgcggtgct cccagggaaa
    11281 cgggcgtggc gggccggcag ccgccagggg tgtgacagca accccgcgcg aagggatgcc
    11341 ggcgaaggct tgagttcgtc ggcctgcccc gctatatacc ggcggcttgg cttagaggaa
    11401 agttcccgtg aaaaaggata tccacccggc ttaccacgag atcaacgtgg tgatgaccga
    11461 cggctcgacc ttcaagacgc gctcgacctg gggtgccgaa ggcgacacgc tgacgctcga
    11521 cgtcgatccg aagtcccatc ccgcctggac cggcggctcg atgcagctgc gcgagcgtgg
    11581 ccaggtggag aagttcaacc ggcgcttcca gagctttcgc cgcaaggcct gatcgcgcgc
    11641 tacgcatcat tcggagacga cgatgaaggt tcgcaattcg ctgaagacgg cgaggacccg
    11701 ccacaaggac tgccgtctcg tgcggcgcaa ggggcgcgtc tacatcatca acaagaagga
    11761 cccgcgcttc aaggcccgcc agggctgaag ccggcgccct cgacgtggac aaaagccggg
    11821 cctggtcccg gctttgttgt ttcgaggtga agggcaggca tgggcagagc cgggctggat
    11881 aagctcttgc gggcgatgct ggatgcggcg gttgcggtcg ccatgccgga tcgcgccgtg
    11941 ccggcacatc tgccgatgcc tccaaagggt cgcacgatcg tgatcggcgc cggcaaggcg
    12001 agcgcggcca tggcccgggc cgtcgagcag cactggccgg gcccgctcga gggtctcgtg
    12061 gtgacccgct acggccacgc cgtgccgtgc gagcacatcg agatcgtcga ggcggcgcat
    12121 ccggtgccgg acgcggcggg cgaggccgcg gcggcacgca tcctcgccat ggtcgagggt
    12181 ctcacggagg acgatctcgt cctggcgctg atctcgggcg gcggctcgtc gctcctggcc
    12241 ctgccggcgc cgggcctcgg cctcgaggac aagcaggccg tcaaccgcgc cttgctgcgc
    12301 tcgggcgcct cgatcgacga gatgaactgc gtgcgcaagc acctctcggc gatcaagggc
    12361 ggccggctgg ccgccgcggc gcacccggcc cggctggtgg cgctcatgat ctcggacgtg
    12421 ccgggcgacg acccggccgt gatcgcgtcc ggccccaccg tgccggaccc gaccacccgc
    12481 gcggatgcgc tggccctggt cgagaaatac gggatcgagc tcgccccgca tgtgcggcgc
    12541 catctcgagg atcccgcctg cgaaagcgtc aagccgggcg acccgcggct cgagcgggcg
    12601 gagacggtca tgatcgcgac gccgcaggcc tcgctcgagg cggcggcgga agtggcgcgc
    12661 gccgccggca tcaccccctt gatcctcggc gacagcatcg agggcgagag ccgcgacgtc
    12721 gccaaggtga tggccgccat cgccaagcag gtgatccgcc acggccagcc ctgcccgcgg
    12781 ccctgcgtgc tgctctcggg cggcgagacg acggtcaccg tccggggcga cggcagcggc
    12841 gggcggaacg tcgagttcct cctggccctc gccgtggcgc tcgacggcct ggcgggggtg
    12901 agcgcgatcg ccgccgacac ggacggcgtc gacggggccg ccgaggtggc cggcgcctgg
    12961 atcgacgacg cgaccctggc gaaggcggcg tcgctcggcc tcgtcgccaa ggccagcctc
    13021 gcgcgcaacg acggccacac gttcttcggg accctgggcc agcaggtcgt caccgggccg
    13081 acgctcacca acgtcaatga tttccgggcc gtgctgatcg actgagtgcc ggacagcacc
    13141 tcgccgccgg cgcaacacat cgccgcgaaa attcgataca atcgcgcgac atgtattggc
    13201 ttgctcgatg ctgaaccccc gctaactcta gcgcatccac ccgagcccgc gtcggagacg
    13261 gattcttgtc gaccgttcgt cagatcctgc tgtcgctgct gctcctgggc ggggcggtcg
    13321 gcggctatta cttctaccat catctgacgg cggcgagcgt catccccggc gacggcaaag
    13381 gcggcgggcg gacgatctcc gtcgaggtcg cgcgagcggt gcgcgagacg gtgcgggacc
    13441 gggccgaggc ggtcggcacg acgctggcca accaggcgat cgaggtggtg tccctggcct
    13501 cggggcggat caccgagctg ctgttcgagc cgggccaggc ggtcgaggcc ggcgccgtgc
    13561 tggcgcgcct cgacgatgcc atcgaagagg cggatctcga cgaggcgcgg gcgatggcga
    13621 aggaagcgca gtcggcgctg gaccgtgccc ggcggctgcg ggccagcaac aacatcgccg
    13681 aggcgaccgt ggtcgagctg gaggccgccg ccgccgccgc ccaggcgcgg gtcgaccggg
    13741 cggcccagcg gctcgaggac cggctggtcc gcgccccctt cgacggggtc gtcggcattc
    13801 gccgggtcga tctgggagcc agggtcgaca acgacacggt gatcaccacg ctcgacgatc
    13861 gaagccggat gatcgtcgag ttcatgctgc cggaattctt ctacggccgc atccagccgg
    13921 gcctcgaggt ccaggccagc agcaccagct tccccgaccg cagcttcgcc ggcacgctca
    13981 ccagcatcga cacgcgcgtc gacgtcacga gtcgcgcctt tcgcgtgcgg gcctcgctcc
    14041 ccaaccccga tctcctcctg ccggcgggcc tcttcgtgct ggtcgacatc acgctcggcg
    14101 agcgcgacgc ggtcatcgtg ccggaggagg ccgtggtcag cgaggccgat cgcagcttcg
    14161 tctatgtggc agggccggag cgggccgagc ggcggctcgt cgagctcggc gcgcgcagcc
    14221 tcggcgtggt cgagatcgag aacggcctcg aggccggcga gctggtcgtc accagcggtc
    14281 tgcagcgcct gcgcgacggt gtcgccatca gctacgaggc gccgaaggcc atcgatcccg
    14341 agcggatcgc cctgccgtcc ggagacatcg ggtgacactc tcggagctca gcatccgtcg
    14401 ccccgtcctc gcgaccgtgg tcagcctgct catcgtcgtc ttcggcatcg ccgcgctgag
    14461 ccgcctgccg gtgcgcgagc tgcccgacgt cgaccgcgcc gtggtgagca tcacgacgag
    14521 ctacaccggt gccgcgccag agatcgtcga caccgacatc accgaggtga tcgagggggc
    14581 ggtggccggg gtttccggcg tgcgcgagat cacctcggag agccgccgcg ggcggggccg
    14641 cacgaccatc accttcaacg gcgaccgcga tatcgacgag gcggccaacg acgtgcggga
    14701 cgccgtcgcc cgggtgcgcg cccgcctgcc cgacgacgcg gacgagccgc agatcgtcaa
    14761 gagcgacagc gacgccgacc cggtgatgcg cctcggcatc gtctccgacc ggatgagccc
    14821 ggcggagatc accgactatg tcgagcggtt cctggtcgac cggctggcga ccctggatgg
    14881 cgtcgccaat gtcgagatca acggccagcg gcgcttcgcc atccgcatct ggctcgaccg
    14941 ccgggcgctc gccgcccgcg acctgacgac cgccgacgtc gaggcggcgc tcaggcgaag
    15001 caatctcgag ctgccggcgg gcgaggtcga gtccaccgcg cgcgagctga ccgtgcgtct
    15061 cgacagccgg ctgcgcgatg tcgagagctt tcgccggctg gtgatcgcga cggcgggcgg
    15121 ctacccgatc cgcctcgccg acgtcgcccg ggtggagctc ggcgcggagg acgattcgac
    15181 cttcgtccgc tcggacgggc ggcccgccgt cggcatcaac gtgctgcgcc agtcgcaggc
    15241 gaacaccatc gccatctccg acgcggtgcg ggcggagatc gagctcctgc ggccgatcct
    15301 gccagccggc atggacatca tcgtcggctc ggacgacgcg atcttcatcg ccgcctcgat
    15361 ccgcgaggtg ctgacggcgc tcggcatctc gatcatcctc gtggtgctgg tcatctttgt
    15421 cttcctcctg tcgctgaggg cgacgctggt cccggccgtc accattcccg tcgcggtcgt
    15481 cggcagcttc atcctgatcc aggcgctggg cttctcgatc aacgtgctga cgctgctggc
    15541 gctgatcctc gccatcggcc tcgtcgtcga cgacgccatc gtcatgctgg agaacatcca
    15601 gcggcggatc gaggcgggcg agaagccgat cacggcctcg gtcctggggg cgcggcaggt
    15661 gaccttcgcg atcatcgcca cctcgctcac gctgatcgcc gtcttcgtgc cgctctcctt
    15721 catgcagggc caggtgggcc ggctcttcat cgagttcggc ctggtgctcg ccgcggccgt
    15781 cctgatctcg accttcgtgg cgctgacgct ctgccccgcc ctctgctcgc gcgtgctgaa
    15841 ggggaggagc gccggcgccg ccgggacgac cggcggcggc cgggcggcgc gggcggtcgg
    15901 caatgtctac cgaaggctcc tgctgcgggc gctcgcggcg ccgctgccgg tcctggtcat
    15961 gggcctcctg ctcgccgcca ccagctatgg gctctacgtc gcactgccgc aggagctgac
    16021 gcccatcgag gatcgcggcg ttttcttcgt gcccgtgatc gcgccccagg gcagcaccgt
    16081 cacctacacc gacgagcggg tggccgaggt cgagggcgtg attcgcccgc tgctcgagga
    16141 cggcgaggtc gagcgcctgt tcgccatcgc cgggtcgttc aattccaaca accgcggctt
    16201 cgtcgtcggc cggctcgccg actggggcct gcgcgatcga tcccagcagg ccatcatcaa
    16261 cgagctggcg ccgaggatct cgggcatcac cggtgcccgg gccttcccgg tgagcccggc
    16321 cgggctcggt ctcggcggct cgagctcgcc cttgcgcgtg gtcatcggcg gccccgactt
    16381 ccaaagcgcg aaggagtggg ccgatgccat cgtcgagcgc gccgaggcga acccgcagct
    16441 cctcaatctc gacgtcgatt tcgaggagaa ccagccgcag ctgacgctgc gggtcgaccg
    16501 cgaccgggcc gacgacctgg gcatctcgct cgagaccatc gcccagacgc tgcagaccct
    16561 gctggcgtcg cgcgaggcca ccgagttcat cgaccgcggc cgcgagtacc cggtgatcct
    16621 ccaggcccgg gcggaggacc ggcgcagccc gaccgacctc gagaacatct tcgtgcgcgg
    16681 cacgaggagc ggcacgctgg tgcccctgac cgcgctggtc cttgccgagg agggggcgtc
    16741 cgccccggcg ctgcgccgct acgaccgcct gccggcggtc gccatcaccg ccgcgctcgc
    16801 cgacggctac gatctcggct cggccatcgc ctatatcgag gcgatcgcgc tggagacgct
    16861 gcccgaggag gcgcgcctcg gctttgccgg ccagagccgc gagttcatcg aggccagggg
    16921 cggcatgctc ttcaccttcg ccatggcgct gctcatcgtc ttcctcgtgc tcgccgccca
    16981 gttcgagagc tggatccatc cgctcatcat catgctcacc gtgccgctcg ccgtggcggg
    17041 cgccctcctc gccctctacg tcaccggcct ctcgctcaac gtctacagcc agatcggcat
    17101 cgtgctgctg gtcggcctga tggcgaagaa cggcatcctc atcgtcgagt tcgccaacca
    17161 gctccgcgac gagggccatg gggtgcgcga ggcggtggtc gaggccgccg tgctgcgcct
    17221 gcggccgatc gtcatgacgg tggtctcgac cgtgctgggt gccgtccccc tcgtcatcgc
    17281 caccggtgcg ggggcggaga gccggatcgc catcggcacg gtgatcatcg gcggcctcgg
    17341 cctcgcctcg gtgctgacgc tggtcctgac acccgtgctc tacgacctgc tcgcccgctt
    17401 cacgcggccg gcgagcgcca tcggccgcga gctcgaggcc gagctgtcgg ccggcctcaa
    17461 tggccgacgg acgacgagag caggttgacg atcagcaccc cggcgacgat caggccgagg
    17521 ccgacgatgg cccagagatc gagccgctgg ccgaagacga cgagcccgac gagcgagatc
    17581 agcacgatgc cgaggccgga ccagatcgcg taggcgatgc cgacaggcat ggtgcggagc
    17641 acgagggcga ggaagaagaa ggcgagcccg tagcacacca cgaccgcgac cgacggccag
    17701 aagcgcgtga actgctgcga cgcctgcagg gccgtggtgc ccgcgacctc gcaggcgatg
    17761 gcgacgacga gctgcagata gaccatggcg acatcatcca gccggccggc gcggccggcc
    17821 atgttgcgag cccgagcatg acggtgatct actacctctc ccatcccgag gtgaccatcg
    17881 atccggcgat cccggtgccg ggctgggggc tctccgccac gggtcgggca cgggtgctgg
    17941 cggtccgcga cgccgcctgg ctgcgggcct gccggcggat cgtcaccagc gccgagcgca
    18001 aggccgtgga gacggccgag ctcgtcgccg agacatcggc ggcggagatc gtggtgcgcc
    18061 ccgccatgca cgagaacgac cggtcggcca cgggcttcct cgtgcccgcg gagttcgagc
    18121 aggtggccag cgaattcttc gcccggccgg aggagtcggt gcgcggctgg gagcgcgcaa
    18181 gcgacgccca ggcgcggatc gcgggcgagc tcgaggcggt gctggcgggc cacgacccaa
    18241 cccttcccct cctcctcgtc ggccatggcg gggtcggcac tttgctctac tgccggcacg
    18301 cggggcttgc gatcgaccgc cggcacgacc agccgaggtc cggctccgtc ttcgccctcg
    18361 atgcccggag cctggcaccc ctcttcacct ggcggccgat ggaggaggtc gagcgggtgc
    18421 tcgggccttg ctaagccgcg cctttcgcgg ccatgttgcg gcggggagac aacgcaggag
    18481 atcgaggcgt ggccagggcc tatgtctgcg gcacgctgga cagcaaggcg acggagctag
    18541 gcttcatcca ggcctgcctc gaggccgcgg gcgttgccac ctgcctcgtc gatctcggca
    18601 ccagcggcga ggccgctccg gccgacgttg ccgcccgcga ggtcgccgcc catcacccga
    18661 agggcgaggc agccgtcttc accggcgacc ggggcagcgc cgtcggcgcc atggccgagg
    18721 ccttcgcccg cttcgtcctg acccgcgacg atatcggcgg catgatcggc gccggcgggt
    18781 cgggcaacac ggccctcgtc acgccggcca tgcgcgccct gccggtgggc gtccccaagg
    18841 tcatggtctc gaccgtggcc tcgggcaatg tggcacccta tgtcggcccc gccgacatct
    18901 tgatgctgca cgccgtggcc gacgtgcagg gcctgaacgc catcacccgc cgggtgctcg
    18961 gcaacgccgc ccatgcgctg gccggcatga tgctgcaccc gattccggcg cctgcggcgg
    19021 ccacgaagcc ggccatcggg ctcaccatgt tcggcgtcac cacgtcctgc gtgcagcagg
    19081 tgacgcgagc gctcgaggcc gactacgact gcctcgtctt ccacgccacc ggcacagggg
    19141 gacaggcgct cgagaagctg gtagccagcg ggctggtcgg cgccatgctc gacatcacca
    19201 cgacggagat cgccgacctc ctggtgggcg gtgtcctgcc ggcgaccgag gatcgcctcg
    19261 aggcggtgat ccagaccggc atcccctatg tcggctcgct gggtgctctc gacatggtca
    19321 atttcggtgc catggagacc gtgccggagc gctttcggga ccgccgcctc tacgtgcaca
    19381 acccgcaggt cacgctgatg cgcacgacgg tcgaggagaa cgcccgcttc ggtgcctggc
    19441 tcgcggcccg gctcaaccgc atggaaggcc ccgtccgctt cctcgtcccc gaaggcgggg
    19501 tctcggcgat cgacgccccg ggccagccct ttttcgatcc ggacgcggat gcggcgctct
    19561 ttgccgcgct cgaggcggat ttccgccccg cgccgaaccg gcgcctcgcc cgcgtcgccc
    19621 acaacatcaa cgatgcggcc ttcgccgccg cctgcgtcgc cgccttccac gagatcacga
    19681 ggacaagctg aatgccccgt ttcgagcgcc aggcgctggt cgagaagttc caggacatgg
    19741 tgcgccggcg cgtgccgatc atcggcggcg gtgccggcac gggcctcagc gccaagtgcg
    19801 aggaggcggg cggcatcgac ctcatcgtca tctacaattc cggccgctac cgcatggccg
    19861 gcaggggctc gctcgccggg ctcatggcct atggcaacgc caacgacatc gtcgtcgaga
    19921 tggccaggga ggtgctgccg gtggtgaaga agacgccggt gctcgccggc gtcaacggca
    19981 ccgacccgtt catgctcagg gacaggttcc tgcgcgacct caaggacatg ggcttcgccg
    20041 gcatccagaa cttcccgacg gtcgggctct tcgacggtgt gatgcgcacc aatctcgagg
    20101 agaccggcat gggctacggg ctcgaggtcg atctcgtccg ggccgcccat gagctggaca
    20161 tgctcacgac accttatgtc ttctcggccg acgaggccgt cgccatgacc gaggccgggg
    20221 ccgacctcat cgtcccgcac atggggctga cgaccggcgg cacgatcggg gcggagacgg
    20281 cggtgaagct cgaggattgc ccccgcatca tcgacgagtg ggcagcggcg gcgcggcggg
    20341 tccggtcgga cgtcatcgtc atctgccatg gcgggccgat cgccacgccc gaggatgcgg
    20401 ccttcgtcct cgcccgcacc gagcactgcc acggcttcta cggcgcctcg tcgatggagc
    20461 ggctgccgac ggagaaagcg ctcaccgccc aggtcgcggc cttcaaagag atcggcttcg
    20521 gcgcatgagc gccacggtcg gcatcctcgt cgtctcgcat tcgcccaagg tcgccgaggg
    20581 tgcggtcgac atggtgcggc agatggtggg cgacgaggtc gcctgcaccg gcgtcggcgg
    20641 cgatccggcg ggcgggctcg gcacggacgc ggcgaagatc ctggccggca tcgaatccgt
    20701 ctggtcggag gccggggtcg cccttctggt cgatctcggc ggggcggaga tgaacagcga
    20761 gatggcgatc ctgatgctcg acgaggcacg ccgcggccgg gttcgcattg ccgccgcacc
    20821 ggtggtggag ggggcggtga tcgccgcgac cgaggcctcg gccggcagca cgctggacga
    20881 ggtcgtggcg gcggcggagg agtggcgctg atggcggccg agcgggcgcg cgtgagggtt
    20941 aaccacccga ccggcctgca tgcccgcccg gcggtcaagc tcaccaggct ggcgaagggc
    21001 ttcgaggcat ccatccgcct gcgcggcctg ccggacggcg cctggatcga cgcgaagagc
    21061 atcgtcaagg tcatggcgct caagctcaag accggcaccc tgatcgagct cgaggccgag
    21121 ggcgcggacg ccggagccgc gctggagggg ctcacgggcc tcgtcgagcg caatttcgac
    21181 gagcatggct gaaaagcact acaagggccg cgtggccgca ccgggtctcg ccctcgggcc
    21241 gctcttccac ctgcccgatc tcgcggtggc ggcagaccga ccggccggca cgccagaggc
    21301 ggagatggcg cggctgacgg cggccatcgc cgccgccagg gagcagcttg cgctgctgct
    21361 cgcggaaggc gaggagatgg cagccgagat cctggggttc cagctcgaga tgctggacga
    21421 tccggccctc gtcgagacgg cggcggcact ggtggccgag ggagccccgg ccgtccgggc
    21481 gtggcaagag gggctcgccc cgcaggtcga ggtcttcgcc ggcgacgacg acctctattt
    21541 ccgcgcccgg gccgccgatc
//
LOCUS       DAOTFI010000055        21540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2917_length_21540_cov_6.611869, whole genome shotgun sequence.
ACCESSION   DAOTFI010000055 DAOTFI010000000
VERSION     DAOTFI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21540
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2917_length_21540_cov_6.611869"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="P5558_07460"
     CDS             complement(<1..882)
                     /locus_tag="P5558_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HRY24182.1"
                     /translation="MTSKQIHELLPVATLTAEDRIVVSTASGNLTRHATIASIAARMP
                     GAGAVDRRISDKLSEAVSVRDFGAVGDGVANDAPAFEAALGAHLAVHVPAGRYRLAST
                     VMVKPGRTITGDGRQATVLVAEGPLALAFQRNAGAYAIDSSSADDWCRSSLRGLTIRM
                     SAGGIQAIGHEFRIDDVNFFGGAAAGWCIDMVDANECVVNRINAGYGGGTRHQMNANG
                     VRWRALKAGVNYGDSLVSEVSIKLGATNTTGVLIDGHSADPARLINNMTLKRIQVNAP
                     TGGGGSESLSGTVGIKLW"
     gene            complement(1054..1380)
                     /locus_tag="P5558_07465"
     CDS             complement(1054..1380)
                     /locus_tag="P5558_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24183.1"
                     /translation="MSLLLPVDDNGNSLPVLGFDPLGTQKVTVSSSSERIPAALDDDI
                     QLVTLIATGPCRFEIGDNAVTADRNASPFLYPGIYVDVPLRRSEQHVAFIAEADDCIA
                     YVIGRR"
     gene            1562..2761
                     /locus_tag="P5558_07470"
     CDS             1562..2761
                     /locus_tag="P5558_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY24184.1"
                     /translation="MHASRRLLIYSHDSFGLGHLRRCRAIAHDLVDRYPQLSVLILSG
                     SPIIGSFDFKSRVDFVRVPGVIKLKNGDYTSLKLNINLSKTLEIRESIIAHTASIFDP
                     DMFLVDKEPLGLRGEVEETLDLLTRRGRRCVLGLRDVMDDPHALYQEWERKGVYPALD
                     RYYHDIWVYGVPEMFDPISEIPGMRRVADRVSFTGYIERQATETDRLAPGLQLPDKPY
                     ILVTTGGGGDGAGLIDWVLRAYESDMTIAYPALLVFGPFMHLEQRRQFQERVDRLSNV
                     FATTFQSNLEALYDRAVGVVAMGGYNTFCEILSFNKPALLVPRTEPRQEQVIRAERAA
                     KLGLVRVLLQDDRLPPVAMATSLHAICNWPRPGAALRQSLLGGLDRIAELCAPFIDPQ
                     RRRARSA"
     gene            2767..4017
                     /locus_tag="P5558_07475"
     CDS             2767..4017
                     /locus_tag="P5558_07475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY24185.1"
                     /translation="MAASIAVVVKGYPRLSETFIAQELLALEQAGLRLLIVSLRHPTD
                     PARHPIHDEIRAPVTYLPEYLHQEPARVAAGLLYAIRHRRFGRVAGLFFRDLRRDPTA
                     NRLRRLGQALVLARELPPSVTCIYAHYLHTPTSVARYAARLLGLRFAISAHAKDIWTT
                     PAWEKREKLVDAAWVTTCTEMGFGHLHSLAPTARLLHLPHGVDLARLPRPPARDGATG
                     VSPGQPIELVTVARAVEKKGLDTLLEALAMLPPTIHWRWRHIGAGPLQPCLRRQAEEL
                     GIDDRIEWLGATPHDAVVEALARADIFCLALRTAADGDRDGIPNVVAEAMSQGLPVVC
                     TTGGAVAELVEDGRTGLLVPAGDAVALGAAIARLVTDAGLRHALGATGRVVVETRFAA
                     GAAQARLARELEALAEKNEVARCA"
     gene            4074..5165
                     /locus_tag="P5558_07480"
     CDS             4074..5165
                     /locus_tag="P5558_07480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY24186.1"
                     /translation="MARAFVALLHALGHEVEIASSLRSYDREGDPERQMAIRAAAEMK
                     AARLIADWRSQAKPAPDLWFTYHSYHKAPDWLGPAVANELRMPYVVAEASVAGKQANG
                     AWALGHRATIEGINSAAKVLAMTRVDAVDLAKWLSETRNLVLFPPFLAQRLKPSAEPT
                     AARRGLSSALGLAMAPVWLVTVAMMRRDIKTESYRLLARALTAMHRDNWRLLVIGDGE
                     NAVEVRAHLTSAAGDRVRFLGQLDSEALATTLCAADVFVWPALREAYGMAILEALSLG
                     LPVVACDGGGVSDLVEHEGNGLLAKERSATGLATCLDRLVDDPALRAGLAARARAGFE
                     ERHSPGAAAARMASILASVAGAEGRSPCA"
     gene            5156..5746
                     /locus_tag="P5558_07485"
     CDS             5156..5746
                     /locus_tag="P5558_07485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY24187.1"
                     /translation="MRLIVLRHGRTAWNVQGRLQGRVDIDLDPVGRREVSDWRCPEGW
                     RDLPCWVSPLRRARQTADLLGRDDVRVAPELIEMDWGSFEGHRLVDLRVEHGADLATN
                     EARGLDFRPPGGESPREVMARLVPWLRSRADGREGALVVTHKGVRRALLALASGWDMR
                     GPPPFKVGNDAALELEVDVSGRPTVVGVVALRGCHA"
     gene            5739..6488
                     /locus_tag="P5558_07490"
     CDS             5739..6488
                     /locus_tag="P5558_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24188.1"
                     /translation="MPEAPQPSKRDALLWWRDDDAGCIAPTLAPLLDLSFAGTRPIGL
                     AVVPAWLDDATMRLIRGEPNAQVLQHGWTHASHALGDEKSIELGGDATTADCLDLLRA
                     GAARLDSAFGDAFLPILVPPWNRIAGRVLERLGEVGYAGLSTFADDLRGAVFGLVHIN
                     AHVDIIDWRKDRRMKPVATLLAEVDDLLAQGAPVIGILSHHLVMSVDDVARLGHFFME
                     LEARYRCRWVAPRALFADPGKLSDEPADRRS"
     gene            6463..8310
                     /locus_tag="P5558_07495"
     CDS             6463..8310
                     /locus_tag="P5558_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24189.1"
                     /translation="MNPLIAVRDLHVSFAADEGTVEAVRGVSFDIAANRTTAIVGESG
                     SGKTVVAHAILGILPKVARIGGGSILFDDPLQPGGPRDLARMDPDGRERRQIRGGRIA
                     IIFQEPMSSLSPLHTIGDQIAEAYRLHVEDNEEAARRATIEILEKVGFPRADRAFSTY
                     TFELSGGLRQRAMIAMALICRPALLIADEPTTALDVTIQAQILKLIEDLKAELGMAVL
                     FITHDLGVVANIADEVVVMYRGRVMEKGPSRELIRSPRHPYLQALLHAVPTIGMDPHV
                     RLQSISDVEADGRGRAVQHAPHLPTTDWLLRVEGLCKTFVGRRTGLLGAERQIVRAVD
                     DVSFTVHRQECLALVGESGCGKTTVSKLIMRGLTPDEGRILFHEGAAEIDVLALDDKA
                     LLQLRTKIQYIFQDPFGSLNPRMTVEEIVAEPLLIHGIGDAASRAARVAELLDAVGLN
                     KRHSRRYPHSFSGGQRQRIGIARALALEPSLLLCDEPVSALDVSVQAQILNLLKDLQA
                     ELGLTYLFVSHNLAVVRYLAHRVAVMCAGRIVELANTDSLFDDPQHPYTQALLAAVPE
                     PDLDRPLDFAALLAGRASEPAAWPEPYRLDGHSGTLIACGQGHFVRVGE"
     gene            8307..10142
                     /locus_tag="P5558_07500"
     CDS             8307..10142
                     /locus_tag="P5558_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24190.1"
                     /translation="MNSSGALLAGSVGALLALAAMPQLATAGPPLQPLVAEMDEIGKP
                     GGDLRMLVSRTRDTRLLYVYGFARLVGFTPELELKADILEEFTVEDGRIFTFKLREGH
                     LWSDGHPFTTEDLRFYWEDIALNEVLSPVGPPIQMVVDGELPVVEVIDELTIRYSWSE
                     PNPFFLPEISAASPLFIYAPAHYLKPFHGSYADPEELARLVSEDNARDWAQLFQRRER
                     MSQFDNPDLPTLQPWRLVTPGPAERFIAERNPNYHRVDANGVQLPYIDRIVLDVVDGR
                     LIPIKTGAGDSDLQSRGLAFRDYTFLKESEPRSGLEVRLWPEARGAHLALYPNLNAAD
                     PVWRELFRDVRFRKALSLAIDREAMSQYLYYGLATPANNTVLAESPLYSPEIAAACLG
                     HEPAEANALLDELGLDQRDSRGIRLLPDGRPLELVVETAGEDSEQADVLELVRADFAE
                     LGFLIHTRPAEREVLRNRIFSGEALMTIWYGIENGIPTADMSPREYAPTNQYDQPQWP
                     MWGQYFETKGEAGEPPDDPAAVRLLELFHDWRRATDSAGRATVWGEMLNLYAEQCYTV
                     GLIANVQQPVAVRKTLRNVPVTATYNWQPHGQIGIYRPDTFWYAD"
     gene            complement(10139..10927)
                     /locus_tag="P5558_07505"
     CDS             complement(10139..10927)
                     /locus_tag="P5558_07505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY24191.1"
                     /translation="MSGETGYFIPYRYAAATMPCGYPAIAPIFDAAGDNFHRMLECID
                     SHAADFARFGGEPPAPRFEQDWFPRLDACAAYAMIRQRRPGLIVEVGCGHSTRIMARA
                     TEDSGAATRHICIDPAPRARLQGLAVEHVPATVDRLDHGQLDTIAAADLLFIDSSHIA
                     VAGSDVDRLVNGILPRLRPGAAIHFHDIFLPEAYPEAWAWRGYNEQSLVAALLSGGGY
                     TVEFASAFVIRRMARALASTCVANLPLMAGAVESSLWLRKRQPR"
     gene            complement(10924..11760)
                     /locus_tag="P5558_07510"
     CDS             complement(10924..11760)
                     /locus_tag="P5558_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY24192.1"
                     /translation="MTSPDFTMRFWGVRGTVPCPQEPMLRYGGNTSCIEVKCGDNRLI
                     FDAGTGLRLLGNHLAATGDSSAAHLFFTHTHIDHIAGLPFFRPAYSRNNQFEFWAGHL
                     RAHGSTLQQVLVQLMQPPLFPVPLGIMHACVCFHDFTPGEVFTPYTGVMLHTLGLNHP
                     GGATGYRIEFAGHVMCIVTDVEHRIGIRDEQIVEFIRGSDIVVYDCTYTDEEYPRYVG
                     WGHSTWQEGIRLCDAADAGRLVAFHHDPEHDDAFLDDMARKMARQRPGSLVAAEGLTL
                     RP"
     gene            complement(11848..12336)
                     /locus_tag="P5558_07515"
     CDS             complement(11848..12336)
                     /locus_tag="P5558_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY24193.1"
                     /translation="MSRLDSFIRRLEAQRACIDAAVRLVAEIPGLVFELGLGNGRTYD
                     HMRQALPDRRIVVFERDPQPHPGCVPATGDLIVGDLETVLPEVADRWAGRVALMHSDI
                     GTGDSARNRRMAASLSLLLPQFLVPGGIIVSDQALASPMLGSLTAPPGVPPDRYFLYE
                     RR"
     gene            complement(12471..14681)
                     /locus_tag="P5558_07520"
     CDS             complement(12471..14681)
                     /locus_tag="P5558_07520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRY24194.1"
                     /translation="MVPAQPPVVPPPVPGTNAAETAANYGIAQIGADSAYSAGARGNG
                     VKVAVIDSGISLGHSEFAGRIDTANSIDIVTGNRSTLEDRSGHGSHVAGIIAANADGK
                     GMRGVAPSASLMAIRADLRDSDVCSTPGCGYFDDDVAAALNHARNRGADIVNLSIGKD
                     TPVNGAYRAALEAAVRSGVLVVVAAGNLRQDEPLHPGALADEAGLRGGMLVVGAVDKD
                     GAIFTQTNRAGDVESRYLVAPGVNIYSTYSDGGYRRLTGTSMAAPHVAGAAAALKSAF
                     PSLSMREVAEILLSTADDLGARGRDQVYGNGLVNLDRALAPVGRQQVAGGDTVSGQMF
                     NVATSSLQLGAAFGDALSGNSALANGMVLDAYDRPYRADFRQSIQGRDGTVDFAGRMQ
                     RVNLTHDLPLDALAPSGVMAGLRLSEQQDRPVPGSMRAALTEPADQQSQELDRLMLQA
                     SDLPAGDLRFGVGLSPSSVSSGATTVSASDLFLDAHTLLAPVDGILAKGTGGAWHLTV
                     GDSTELTFGVLDSEALSAPGTETYEHEGRLLSFGADQRLGEQISVRFDYAYVDEATAL
                     LGSSASGAFAFDDGAQSHLGTARFAYRPITTVELFAQATLGVSMLDDEGGMLSDWSTV
                     TSDAFAFGVIADSVFEESDRLGVVVGQPLRVSSASVMIDVPTGRTLDGGIERSQERVE
                     ATPNGREVRLELAYQRMLQAKRAIAGWLVFQHEPGHAEDAEPALGVGIRFTERF"
     gene            complement(15004..15984)
                     /locus_tag="P5558_07525"
     CDS             complement(15004..15984)
                     /locus_tag="P5558_07525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HRY24195.1"
                     /translation="MRAVITRELKGPAGLELAELPAPETGACQVRIAVDAAGVNFADA
                     LMLEGRYQERPALPFVPGLEVAGRIDKVGVGVKGLECGEHVLALVDHGGFAEQAIARA
                     DDVVSLPEGFDPIIAAGFAIAYGTAFGALVWRAGLAKGEVLLVHGAAGGVGSTAVECG
                     KAMGATVIATARGSDRLAIATAHGADHVIDSDAPDLVEQIRALTNGAGVDVVFDPVGG
                     ALFAASLRAIAWEGRIVVIGFASGTIPQIPANILLVKNAAALGFYWGSYRKRDPERLR
                     AGFRRLFEWHEAGRIHPQVSEVLPLARTAEAIERLRRRQAAGKLVIDVRH"
     gene            16094..16612
                     /locus_tag="P5558_07530"
     CDS             16094..16612
                     /locus_tag="P5558_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRY24196.1"
                     /translation="MRNDLARGYFAIGADGISKPMNLGNLVRSANAFGASFVFLVGVR
                     QSVRQMKSDTSHAELQVPVYDVPDVAALSLPRGCRLVGVELVDHAVALPSFRHPLQAA
                     YVFGPERGSLSEGMLARCDHVVRIPTRFCVNVAVAGAIVMYDRMVSLGRFAERPVRSG
                     GPPAGPGTPESR"
     gene            16690..17202
                     /locus_tag="P5558_07535"
     CDS             16690..17202
                     /locus_tag="P5558_07535"
                     /inference="COORDINATES: protein motif:HMM:NF018482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HRY24197.1"
                     /translation="MSRQFVIVAGLLALLSLGGEAFAQSPQFLGNFRDWYLYAYDDSG
                     GKTCYIASKPTEEEGNYRRRGAAAVLVARLPIAGAGEQVSVQPGYSFKPGSTVEIRIG
                     GKRWDFFTQGEHAWANTDDEDRQVIAAMKDGSDMTVRGTSTLDTFSLDTYSLLGFTAA
                     LEALRDACPG"
     gene            17186..18340
                     /gene="rlmN"
                     /locus_tag="P5558_07540"
     CDS             17186..18340
                     /gene="rlmN"
                     /locus_tag="P5558_07540"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRY24198.1"
                     /translation="MPAPADPLPVAGTTAAPAPKPLAADGRPSLIGLDLASLTEVIGA
                     LQVVPERQAALRARQLFHWLYHRGACDFASMSTLPKPLRQALDDGWSVARPDIATAQT
                     AADGTRKWLLRFADGQEVETVFIPEDDRGALCISTQVGCTLTCSFCHTGTMPLVRNLG
                     VGEITAQLLVARDALGEWPTAKEGRLFTNIVVMGMGEPLFNYDNIAGALRLAMDETGL
                     AISRRRITLSTSGVVPRIAQCGRELEVGLAVSLHAVRDELRDELVPLNRKWNIAELLE
                     ACRQYPARRPITFEYVMLHGVNDSDADARELVRLLAGLSAKVNLIPFNPWPGAPYACS
                     SNNRIRAFAAIVEAAGYASPVRTPRGRDILAACGQLKTASERQRRQRVAA"
     gene            18369..20087
                     /locus_tag="P5558_07545"
     CDS             18369..20087
                     /locus_tag="P5558_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRY24199.1"
                     /translation="MKRLIVGFLAVVGALALVVLVAAIAGGIWFTRQFTAGPELPERI
                     VLTLDLGEPLVETVPGDPLATIGLEQPLELGEIVLALERAAADPRVRGLVARLDATDH
                     GMAVAQELRATIDRFRAGGRFARAWADSFGELGPGNEGYYIATAFDEIHLQPGGMVGL
                     TGLLAEIPFVRPLLDRLGIQAEVIRRSDYKTAFDSLVEDGLTAAHAEMMNDLLDQIYA
                     ALVDGVAASRGLAPAEVERLIDAGPFTGEAALAAGLVDGLAFRDEVLDRALAEAGPGS
                     AAVGLAAYARAEPESDGEPPATVALIVAQGTIQRGTAGFDAFIGADDLAAQLAEARDD
                     DAIDAVVLRLDTGGGSAVASETIGREVELLRQAGKPIVVSMGNAAASGGYWIAMGASH
                     IVAEPATLTGSIGVIAGKPVLAGLWEQLGVNWATLQRGENAGYLSLNRPFDALARERL
                     ERAVGALYERFKAGVAEGRGLPPNSVEALAQGRVWLGSEAVGLGLVDELGGILEARAA
                     AARLLELPAGSIPHLQRYPEPPSTFERLRELFDQPSLPGLASLIALWHGALLPGTAEI
                     TLPRFR"
     gene            complement(20793..>21540)
                     /locus_tag="P5558_07550"
     CDS             complement(20793..>21540)
                     /locus_tag="P5558_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY24200.1"
                     /translation="ALVEERLGGCLIGHVSRDGTAIAARERPPLAAAPKQPEKRRGRG
                     RPRKGEIVEKPVRRLATQPGMSLEARLADLPRACSIGNRKNAKGYKEFWRGYRLHIDV
                     ADGDIPVSCLLTGAHVHDSQAAIPLAVMTARRVTNLYDLMDSAYDVREIHDHSRSLGH
                     VPVIDPVPRSKAAKDAWANEKKAREQIAWKPAEARRLAQRTSCERVNSRLKDQFGGNH
                     VRVRGPTKAMCHLMFGILALTVDQLIRLVT"
BASE COUNT         3422 a         7205 c         7239 g         3674 t
ORIGIN      
        1 ccagagcttt atgcccaccg ttcccgacag cgactccgaa ccgccgccgc cggtaggtgc
       61 gttcacctga atccgcttca gggtcatgtt gttgataagg cgggcagggt cggccgaatg
      121 accgtcgatc agcacgcccg tggtgttggt ggcgccgagc ttgatggaaa cctcggagac
      181 caggctgtcg ccgtagttga cgcccgcctt caatgcccgc cagcgtacgc cattggcatt
      241 catctgatgc ctggtgccgc cgccgtaacc ggcattgatg cggttcacga cacattcatt
      301 ggcatcgacc atgtcgatgc accagcctgc ggcagcgccc ccgaaaaaat tcacgtcgtc
      361 gatgcggaac tcgtggccga tggcctggat gccgcctgcg ctcatccgga tcgtgaggcc
      421 gcgcagcgag gagcggcacc agtcgtcggc gctgctcgaa tcgatcgcgt aggcgcctgc
      481 attgcgctga aacgccagcg ccagggggcc ttccgcgacg agcaccgtgg cttggcgccc
      541 gtcgccggtg atcgtgcgcc ccggcttcac catgaccgtc gaggccaggc gataacgacc
      601 cgccgggacg tgcacggcga gatgggcgcc gagcgcagcc tcgaaggccg gtgcgtcgtt
      661 cgcaacgccg tcgccaacgg ccccgaagtc cctgaccgag accgcttccg agagcttgtc
      721 cgagatccgg cgatcgaccg ccccggcgcc gggcatcctg gccgcgatgc tggcgatcgt
      781 ggcgtggcgg gtgaggttgc cgctggcggt cgagacgacg atccggtctt ccgcggtcaa
      841 tgtcgctacc ggcagcaact cgtggatctg cttgctcgtc atgacatgct ccctgttgca
      901 agctgagcgc tgatttcggt gacgatcggg tcgttggtgt cagtgaccag aagcgccgcc
      961 tggttggtcg atggcggcaa cacgatcacc gggctgcgag tggccctgac caaccgtagc
     1021 ccgcgcgctg tcggcgctag cgacatggtc atctcagcgg cggccgatga cgtaagcgat
     1081 gcaatcgtcg gcttcggcaa tgaacgccac atgctgctca ctgcggcgca gaggcacgtc
     1141 gacatagatg ccaggataga ggaacgggct ggcgtttcga tcggccgtta cggcattgtc
     1201 accgatctcg aaccggcagg gtccggtcgc gatcagcgtg accagctgaa tgtcgtcgtc
     1261 cagggcagcc gggatccgct cggagctgct cgatacggtg accttctggg tgccaagggg
     1321 gtcgaacccc agaacgggca gcgaattgcc attgtcgtcg acgggcagaa gcaggctcac
     1381 ggaagctctc cattgcgcag atgggccgat ggcggcgcgg gaactgaacg cattcgacta
     1441 tactaggaac aaaaacctat gtcaatgtgt gggccgctgt ggccggctcc gaaaaacaaa
     1501 tcgctttcgc tgcgccccct gcctctgaca tattccgacg gagcagaacg cctcggatct
     1561 tatgcacgcc agtcgccgac tactgatcta cagtcacgac agcttcggcc tcggccatct
     1621 tcgccgttgc cgggcgatcg cgcacgacct cgtcgatcga tacccacagc tttccgtcct
     1681 gattctctcg gggtcgccca tcatcggcag cttcgatttt aaatctcggg ttgattttgt
     1741 tcgagtgccg ggagtgatca agctcaagaa cggtgactac acgtccttga aactgaacat
     1801 caatctgagc aaaacgctgg agatacggga gtcgatcatc gcccacacgg cgagcatctt
     1861 cgatcctgac atgttcctgg tcgacaagga gccgcttggt ttgcgtggcg aggtcgagga
     1921 aacactcgat ctcctgacgc ggcgtggtcg ccgatgtgtg ctcggcctcc gcgacgtcat
     1981 ggacgatcca catgcgctct atcaggagtg ggagcggaag ggggtctacc cggcgctcga
     2041 tcgctactac cacgacatct gggtctatgg cgtgcccgag atgttcgatc ctatcagcga
     2101 aattcccggt atgcggcgtg tggccgaccg ggtcagcttc accggctaca tcgaacggca
     2161 ggcgaccgag accgaccgtc tcgcacctgg cctccagttg cccgacaagc cgtatatcct
     2221 ggtcacaact ggcggcgggg gcgatggggc agggctgatc gactgggtct tgcgggccta
     2281 cgagtccgat atgaccattg cgtatccggc cctcctggtg ttcggaccct tcatgcatct
     2341 cgagcaacgg cgccagttcc aggagcgggt cgaccggttg tcgaacgtgt tcgccacgac
     2401 cttccagtcg aacctcgaag ccctctacga tcgagcggtt ggtgtcgtcg ccatgggggg
     2461 ctacaacact ttctgcgaga tcctgtcgtt caataaaccg gcacttctcg taccccgaac
     2521 cgagccgcgc caggagcagg tgatccgtgc cgagcgggcg gccaagctcg gcctcgttcg
     2581 agtgctgctc caggacgatc gcctgccacc cgtagcaatg gcgacttcat tgcacgccat
     2641 ctgcaactgg ccgcgccctg gtgcggccct gcgccagagc ctgctcggcg gcctcgaccg
     2701 gatcgctgag ctctgcgcgc cgtttatcga tccgcaacgg cgccgggcgc gctctgcctg
     2761 aacaatatgg ctgccagcat cgccgtcgtc gtcaagggct acccgaggct ctcggaaacc
     2821 ttcattgccc aggaattgct ggcgctggaa caagccggct tgaggcttct catcgtctcg
     2881 ctgcgccacc cgaccgaccc ggcgcggcat cccattcatg acgagattcg ggcacccgtc
     2941 acctacctgc cggagtacct gcatcaggaa ccggctcgcg ttgccgccgg cctgctgtac
     3001 gcgattcggc atcggcgctt cggccgggtc gccggtctct tcttccgcga tctgcggcgg
     3061 gacccgacgg ccaatcgtct gcgccggctg ggccaggccc tcgtgctggc acgcgagctg
     3121 ccgcctagcg tcacctgcat ctatgcgcat tacctccaca ccccgacctc ggtggcacgt
     3181 tacgccgcgc gtctgctcgg cctgcgattt gccatctccg cccatgccaa ggatatctgg
     3241 acgacaccgg catgggaaaa acgcgagaag cttgtcgacg ccgcatgggt gacgacctgc
     3301 accgagatgg gctttggcca tctccattcc ttggcgccga cggcgcgact gctccatttg
     3361 cctcacggcg tggacctcgc ccggctgcct cggccgccgg cgcgtgacgg ggccacgggc
     3421 gttagcccag ggcagccgat cgagctcgtc accgtcgccc gcgccgtcga gaagaagggc
     3481 ctggatacgc tgctggaagc actggctatg ctgcccccca caattcactg gcgatggcgc
     3541 cacatcggtg cgggtccttt gcagccgtgc ctgcgacggc aggccgagga actcggtatc
     3601 gacgatcgca tcgaatggct cggcgccaca ccccacgacg ccgtggtcga agcgttggcc
     3661 cgggcggata tcttctgcct cgcactccgg accgctgccg atggcgatcg cgatggcatt
     3721 cccaacgttg tggccgaggc catgtcgcag ggcctgccgg tcgtctgcac cactggcggg
     3781 gccgtcgccg agctcgtcga ggacggtcgg acgggacttc ttgttcctgc cggggatgcg
     3841 gtagccttgg gtgccgccat cgcgcggctc gtgacggatg cagggctgcg acatgcgctg
     3901 ggcgcaacag gccgcgtggt ggtggagact cgctttgccg ccggagccgc ccaggcacgc
     3961 ctcgctcgcg agctcgaggc actggcggag aagaacgagg tcgctcgatg cgcatagcct
     4021 tttatgcgcc gatgaagccc gttgagcacc cgaccccttc gggagatcgg cgtatggccc
     4081 gcgctttcgt cgcactgctc cacgctcttg gtcacgaagt cgaaatcgct tcgtccttgc
     4141 gcagctatga ccgggagggc gatcccgagc ggcagatggc gatccgggct gccgccgaga
     4201 tgaaggccgc acggctgata gccgactgga gatcccaagc caagccggct cccgaccttt
     4261 ggttcaccta ccattcctac cacaaggcgc ctgattggct cgggccggcc gtggcgaatg
     4321 agctgcgaat gccctacgtt gtcgccgagg cgtccgtggc cggcaagcag gcgaatggcg
     4381 cctgggcgct cggtcatcgc gcaacgatcg agggcatcaa ttccgccgcc aaggttctgg
     4441 ccatgacccg cgtcgacgcg gtcgacctcg ccaagtggct gtcggagacc cgaaatctgg
     4501 ttctcttccc gcccttcctg gcgcaacgcc tgaagccgtc agcggaaccg acagccgccc
     4561 gccgaggctt gtccagcgcg ctcgggctgg cgatggcgcc ggtctggctg gtgacggtcg
     4621 ctatgatgcg ccgggacatc aagaccgaat cctatcggct gctcgctcgt gccctcaccg
     4681 ccatgcaccg cgacaattgg cgcctgctcg tcatcggtga cggtgaaaat gcggtcgagg
     4741 tgcgagccca tttgacgagc gccgctggtg atcgggtgcg attcctcggc cagctcgaca
     4801 gcgaggcgct ggcgacgacc ctgtgcgccg ccgatgtctt cgtctggccg gcgctcaggg
     4861 aagcctacgg catggcgatc ctcgaggccc tgagcctcgg tctgccggtg gtggcctgtg
     4921 acggaggggg cgtctcggat ctcgtcgagc acgagggcaa cgggctgctc gcgaaggagc
     4981 gttcggcgac cgggctggcg acctgtctgg atcgactggt cgatgatccg gcgctgcgcg
     5041 ctgggttggc cgccagagcg cgggcagggt tcgaggagcg gcacagtccg ggtgcggcgg
     5101 cggcgagaat ggcgagtatc ctcgcaagtg tggcgggagc cgaagggcga tcgccatgcg
     5161 cctgatcgtc cttcgtcatg gccgcacggc gtggaacgtg caaggccgct tgcaggggcg
     5221 ggtcgacatt gacctcgatc ctgtcggcag gcgcgaggtc agcgactggc gctgccccga
     5281 gggctggcgc gatctgccct gctgggtcag cccgctgcgc cgggccaggc agaccgccga
     5341 cctgctcggc cgtgacgacg ttcgggtcgc acccgagttg atagagatgg actggggcag
     5401 cttcgaggga caccgtcttg tcgaccttcg ggtcgagcac ggtgccgatc tggcaaccaa
     5461 cgaagcacgt ggcctggatt ttcgtccgcc gggcggcgag agcccgcgcg aggtcatggc
     5521 ccggctggtt ccgtggctgc gttctcgtgc cgatggccga gagggcgctc tggtggtcac
     5581 ccacaagggt gtgcggcgag cactgctggc cctcgccagc ggctgggaca tgcgagggcc
     5641 accgccgttt aaggtcggca acgatgccgc cctcgagctc gaggtggacg tctcggggcg
     5701 gccgacagtg gtcggcgtcg tagcgctccg cgggtgccat gcctgaggcg ccgcagccct
     5761 cgaagcgcga cgcccttctc tggtggcggg acgacgatgc tggctgcatt gcaccgacac
     5821 tggcgcccct gctcgatctg tcgttcgccg gcacgcggcc gatcggtctc gcagtcgtgc
     5881 cggcctggct cgatgacgcc acgatgcggc tgattcgcgg cgaacccaat gcacaggtgc
     5941 tccagcatgg ctggacccat gcgagccacg ccttggggga tgagaaatcc atcgagcttg
     6001 ggggcgatgc gacgactgcc gactgcctcg acctgctgcg ggcgggagcc gcgcggctgg
     6061 attcggcatt cggcgacgcc ttcctgccga tcctggtgcc gccttggaac cggatcgccg
     6121 gccgggtgct ggagcgactc ggcgaagtcg gctacgcggg cctctcgacc ttcgccgatg
     6181 atctccgggg tgccgtgttc gggctcgttc acatcaacgc gcatgtcgac atcatcgact
     6241 ggcgcaagga tcgacgcatg aagccggttg ccacgcttct cgccgaggtc gacgatctcc
     6301 tcgcgcaggg tgcgcccgtt ataggcattc tgtcgcatca tctcgtcatg tcggtcgacg
     6361 acgtggcccg gctcgggcac ttcttcatgg agctggaagc caggtaccgc tgccgatggg
     6421 tagcgccccg cgcgctgttc gctgatcccg gaaagctgtc cgatgaaccc gctgatcgcc
     6481 gttcgtgacc tgcatgtgag cttcgccgcc gacgagggga cggtggaagc ggtccgcggc
     6541 gtctcgttcg atattgccgc caatcgcaca acggcgatcg tcggcgaaag cggctcgggc
     6601 aagaccgtcg tggcacacgc cattctcggc atcctgccga aagtggcgcg cattggcggc
     6661 ggctccatcc tgttcgatga tccgctgcaa ccgggcgggc cgcgtgacct cgccaggatg
     6721 gaccccgatg gccgcgaacg gcggcagata cgtggtggcc ggatagcgat catcttccag
     6781 gagccgatga gctcgctctc ccctttgcac acgatcgggg accagatcgc cgaggcctac
     6841 cggctccacg tcgaggacaa cgaggaagcc gcccggcgcg ccacgatcga gatcctcgag
     6901 aaggtcggct tcccgcgcgc cgatcgggcc ttcagcacct acacgttcga gctctcgggc
     6961 ggcctgcggc agcgggcgat gatcgccatg gccttgatct gccgcccggc gctgctgatc
     7021 gccgacgagc cgacgacggc gctcgacgtc acgatacagg cccagatcct caagctcatc
     7081 gaggatctca aggccgagct cggcatggcg gtcctgttca tcacccatga cctaggtgtg
     7141 gtggcaaaca tcgccgacga ggtcgtcgtc atgtatcgcg gccgtgtcat ggagaagggg
     7201 ccgagccggg agctgatccg gtcgccgcgc catccgtacc tgcaggctct tctccatgcg
     7261 gtgccgacga tcggcatgga tccacatgtg cggctccagt cgatcagcga tgtcgaggcg
     7321 gatggccgcg gtagggcggt gcagcatgca ccgcacctgc cgacgaccga ctggctgctg
     7381 cgggtcgaag gcctgtgcaa gaccttcgtc ggccgacgca ccgggctgct cggcgcggag
     7441 cgccagatcg tcagggcggt cgacgatgtc tcgttcaccg ttcatcggca ggaatgcctg
     7501 gccctcgttg gcgagagcgg ctgcggcaag acgacggtca gcaagctgat catgcgtggc
     7561 ctgacaccgg acgaagggcg tatcctcttt catgaaggcg ccgccgagat cgatgtcctg
     7621 gcgctcgacg acaaggcgct gctgcagctc agaaccaaga tccagtatat cttccaggat
     7681 ccgttcggct cgctcaaccc gcgcatgacg gtcgaggaga tcgtggcgga gccgctcctg
     7741 atccacggca tcggtgacgc ggcgagccgc gctgcccgag tcgccgagtt gctcgacgct
     7801 gtcggtctga acaagcgtca ttcccggcgc tatccgcaca gcttctccgg cgggcaacgg
     7861 caacgaatcg gcatcgcccg ggccctggcg ctcgagccga gcctgttgct ctgcgacgag
     7921 cccgtctcgg cgctcgacgt ctcggtccag gcccagatcc tgaacctgct caaggacctc
     7981 caggcggagc tcgggctcac ctatctcttc gtctcccaca atcttgccgt ggttcgttat
     8041 ctcgctcatc gtgtcgcggt gatgtgcgcc ggccggatcg tcgagctggc caataccgac
     8101 agcctgttcg atgatcccca gcatccctac acccaggcac tgctcgccgc ggtgccggag
     8161 cccgatctcg accggcccct cgatttcgcg gccctgctcg caggtcgggc ctctgaaccg
     8221 gccgcgtggc cggagcccta tcgactggac ggccactccg gaacactgat agcttgtggc
     8281 cagggtcatt tcgtacgggt gggcgaatga acagctcagg cgctttgctg gcgggttctg
     8341 tcggagcact gctggccctg gcggcaatgc cgcagttggc aacggcaggc ccacccctgc
     8401 agccgctggt cgccgagatg gacgagatcg gcaagccggg gggcgacctt cggatgctgg
     8461 tcagccggac gcgcgacacg cgtctgctct atgtttacgg gttcgcccgc ttggtcggct
     8521 tcacgccgga actcgagctc aaggcggata tcctcgaaga attcaccgtg gaagacggcc
     8581 gcatcttcac cttcaagctg cgtgaaggac acctctggtc cgacggacat ccgttcacca
     8641 ccgaggatct gcgcttctat tgggaggaca tcgcgctcaa cgaggtgctg tctccggtag
     8701 gaccacccat ccagatggtc gtggacggcg agctgcctgt ggtggaggtt atcgacgagc
     8761 tgaccattcg ctattcctgg tcagaaccca atccgttctt cctgccggag atctccgccg
     8821 catcgccgct cttcatctac gcgccggcgc actacctgaa gccgttccac gggagctatg
     8881 cggatcccga ggagctcgcg cgtctggtga gcgaggacaa tgcccgggac tgggcgcagt
     8941 tgttccagcg ccgtgagcgg atgagccagt tcgacaatcc cgacttgccg acactgcagc
     9001 cttggcgact cgtgacccca ggtccggcgg agcggttcat cgccgagcga aacccgaatt
     9061 accatcgggt cgacgccaac ggcgtgcagc ttccctacat cgaccgtatc gttctcgatg
     9121 tggtcgatgg ccgcctcatt ccgatcaaga ccggtgccgg cgattccgat ctgcagtcgc
     9181 gcgggctggc ctttcgcgac tatacgttcc tcaaggagag cgagccacgc agcggtctag
     9241 aggtcaggct ctggccggaa gcgcggggcg cgcatctcgc cctctatccc aatctcaatg
     9301 ccgccgatcc cgtctggcgc gagctttttc gagatgttcg tttccgcaag gcactctcgc
     9361 tcgcgatcga tcgcgaggcg atgtcccagt atctctacta cgggctggca acgccggcta
     9421 acaacacggt tcttgcggag agccctctct attcccccga gatcgccgca gcctgtctcg
     9481 gccacgagcc ggccgaggcg aatgcgttgc tcgacgagct agggctcgac cagcgcgatt
     9541 cccgcggcat tcgcctgctg cccgacggcc ggccgctcga gctggtcgtc gagacggcgg
     9601 gcgaggacag cgagcaagcc gacgtcctgg agctggtccg ggccgatttc gccgagctcg
     9661 gctttctcat tcatacgcgg ccggccgaac gcgaggtgct gcgcaaccgg atcttctccg
     9721 gcgaggcgct gatgacgatc tggtacggga tcgagaacgg catcccgacg gccgacatga
     9781 gcccccggga atacgcgccg accaatcagt acgaccagcc gcaatggccg atgtgggggc
     9841 agtatttcga gaccaagggc gaggccggcg agccgccgga cgatccggcg gcggtgaggt
     9901 tgctcgagtt gttccacgat tggcggcggg ccaccgacag cgcgggtcgt gcgacggtgt
     9961 ggggtgagat gctcaacctc tatgcggagc aatgctatac ggttggcctc attgccaatg
    10021 tccagcagcc ggtcgcggtt cggaagacat tgcgcaacgt gccggtgacg gcgacgtata
    10081 actggcagcc gcatggccag atcggcatct atcggcccga tacgttctgg tatgccgact
    10141 agcgcggttg gcgcttgcgc agccaaaggc tgctttcgac ggcgcccgcc atgagcggca
    10201 ggttcgcgac gcaggtcgag gcgagcgccc tggccatccg tcgaatgacg aacgcactgg
    10261 cgaattccac tgtgtagccg ccgccggaga ggagtgctgc gaccagtgat tgctcgttgt
    10321 agccccgcca ggcccacgcc tccggatagg cctctggcag gaagatgtca tggaaatgga
    10381 tggccgcacc cggccgcagc cgtggcagga tgccattcac caaccggtcg acatcgctgc
    10441 ccgccaccgc gatgtggctc gaatcgatga acagcaggtc cgccgcggca atggtgtcga
    10501 gttggccatg gtcgaggcgg tcgacggtgg ccggaacatg ctccaccgct aggccctgca
    10561 accgcgcgcg gggggccgga tcgatgcaga tgtgccgcgt cgcagcaccc gaatcctctg
    10621 tggcacgagc catgatgcgg gtcgaatgcc cgcagcccac ctcgacgatc aggccgggcc
    10681 gccgctggcg gatcatcgcg taggcggcgc aggcgtccag ccgaggaaac cagtcctgct
    10741 cgaagcgcgg cgctggcggc tcgccgccga accgggcgaa atccgcggca tggctgtcga
    10801 tgcattccag catgcgatgg aagttatcgc cggccgcatc gaaaatcggc gcgatcgccg
    10861 gatagccgca cggcatcgtc gctgccgcgt agcgataggg aatgaaatag cccgtctcgc
    10921 cgctcacggt cgcagcgtca gcccttcggc ggccaccagc gaccccggtc gttgtcgtgc
    10981 catcttgcgc gccatgtcgt cgagaaaggc gtcgtcgtgc tcggggtcat gatggaacgc
    11041 cacgaggcga cctgcgtcgg ccgcgtcgca cagcctgatg ccctcctgcc acgtggagtg
    11101 cccccacccc acatatcgcg ggtactcctc gtccgtgtag gtgcagtcgt agacgacgat
    11161 gtcgctgcca cggatgaact cgacgatctg ctcgtcacgg atcccgatac gatgctccac
    11221 atcggtcacg atgcacatca cgtggccggc gaattcgatt cgatagccgg tggcaccgcc
    11281 ggggtgattc agaccgagcg tgtgcagcat cacgccggta tagggggtga acacctcgcc
    11341 cggcgtgaaa tcatgaaagc acacgcaggc gtgcatgatg cccagcggca cgggaaagag
    11401 cggcggctgc atgagctgca cgaggacctg ctgcagcgtc gagccgtgcg cgcgcaaatg
    11461 ccccgcccag aactcgaact ggttgttgcg gctgtaggcc ggccggaaga atggcaagcc
    11521 ggctatatga tcgatgtggg tgtgcgtgaa gaacaggtgg gcggcgctgc tgtcgccggt
    11581 tgccgcgagg tgattgccga gcaggcgcaa tcccgtgccg gcatcgaaga tcagccggtt
    11641 gtcgccgcat ttgacctcga tgcaggacgt gttgccaccg tagcggagca ttggctcctg
    11701 cgggcatggg accgtgccgc gcacgcccca gaaacgcatc gtgaagtcgg gcgatgtcac
    11761 cgccttcgcc cggcgggcaa tggtgtcggt cggatgcggg ctgtacggtt ggccacggcg
    11821 cgccggcaag ggcggaccgt ggcccgatca acggcgttca tagaggaaat accggtcggg
    11881 aggcacaccc ggcggtgcag taagcgaacc gagcatcggt gaggctagag cctgatcgct
    11941 gacgatgatc ccgcccggga ccaggaactg cggcagcaac aacgagagtg acgcagccat
    12001 tcgacgattt cgagcggagt cgccggtgcc tatgtcactg tgcatgagag cgacgcggcc
    12061 ggcccatcga tcggcgactt ccggcaggac tgtttccaga tcgccgacaa tcagatcacc
    12121 ggtcgccggc acacagcctg ggtgcggttg tggatcgcgc tcgaagacga cgatccgccg
    12181 gtcgggcaat gcttgcctca tatggtcgta cgttcgtccg ttgccgaggc cgagctcgaa
    12241 caccaaaccg ggaatctcgg caaccagccg aaccgcggcg tcgatgcagg cgcgttgggc
    12301 ttcgaggcgt cgtatgaagc tgtcgagacg gctcatcgca cgcgccgagc ccgccccctc
    12361 atacaatcac ccttcctacc gaccacaact gatttcacga tccaccacgg cgggctgtga
    12421 cgttcgtcac attgggcacc gaatagagat gtcctgcccg cgataccgcg tcaaaacctc
    12481 tccgtaaagc gaatgcccac gcccagcgcc ggttcggcat cctccgcgtg acccggctcg
    12541 tgctggaaca cgagccatcc cgcgatggcc cgcttcgcct gaagcatccg ttgataggcg
    12601 agctcgagcc tgacctcgcg accattcggt gtcgcctcga cgcgctcctg gctgcgctcg
    12661 atcccaccat cgagcgtgcg gccggtcgga acgtcgatca tcaccgatgc cgagctgacc
    12721 cgcagcggct ggccgaccac cacgcccagc cgatcggatt cctcgaacac gctgtcggcg
    12781 atcacaccga acgcgaaggc atcggaggtc actgtgctcc agtcgctcag catgcctccc
    12841 tcatcgtcga gcatggagac gcccagggtc gcttgcgcga aaagctcgac ggtggtgatg
    12901 ggccggtagg cgaagcgggc cgtgccgaga tgcgactgcg cgccgtcgtc gaaggcgaag
    12961 gcgccgcttg ccgagctgcc gagcagtgcc gtcgcttcgt cgacataggc gtagtcgaaa
    13021 cgcacggaga tctgctcgcc gagccgctgg tcggcaccga aagacagcag ccgtccttca
    13081 tgctcgtatg tctcggtgcc gggcgcggac agggcttcgc tgtcgagaac gccgaacgtc
    13141 agctcggtcg agtcgcctac cgtgaggtgc catgccccgc ctgtgccctt ggcgaggatg
    13201 ccgtcgaccg gtgccaggag ggtatgggcg tcgaggaaga ggtcgctcgc cgacacggtc
    13261 gtggcacccg agctgacgct cgagggcgag aggccgacgc cgaagcgcag atcgcctgct
    13321 ggcaggtcgc tggcctggag catgagacga tcgagctcct ggctctgctg gtcggccggc
    13381 tcggtgagcg cggcacgcat cgagccgggc accggtcgat cctgctgctc gctcaggcgg
    13441 agcccggcca tgacgccgct cggcgccagc gcatcgagcg gcaaatcgtg ggtcaggttg
    13501 acccgttgca ttcgcccggc aaagtcgacc gtgccgtcgc ggccttggat cgactgtcgg
    13561 aagtccgccc gatatggccg gtcataggcg tccagaacca tgccgttggc gagggcgctg
    13621 ttgccgctca gcgcatcgcc gaacgccgcg cccagctgca ggctggaggt cgcgacattg
    13681 aacatctgcc cggaaaccgt gtcgccaccc gccacctgct gccgtccgac gggtgccagc
    13741 gcccgatcca ggttgaccag accattgccg tacacctggt cgcggccacg ggcacccaga
    13801 tcgtcggcgg tggagagaag tatctcggcc acctcgcgca tcgacaatga aggaaaggcg
    13861 ctcttgagcg ctgccgcggc tccggcgacg tgcggggcgg ccatcgacgt cccggtgagc
    13921 cgacgatagc caccgtcgct ataggtgctg tagatattga cgccgggggc gacgagatag
    13981 cgcgattcga cgtcgccggc ccgattggtc tgggtgaaga tggcaccgtc cttgtcgacg
    14041 gcgccgacca cgagcatgcc gccccggagg ccggcttcat cggccagcgc acccggatgc
    14101 agcggctcgt cctggcgcag gttgccggcg gcgacgacga cgagaacgcc gctgcggacg
    14161 gcagcttcca gggcggcgcg ataggcgccg ttgaccggcg tatccttgcc gatgctgagg
    14221 ttgacgatat cggcgccgcg gttgcgcgcg tggttcagcg cggcagccac atcgtcgtcg
    14281 aagtagccgc agccgggcgt cgagcagaca tcgctgtcac gcaggtccgc ccggatggcc
    14341 atgagcgagg ctgatggtgc caccccccgc atccccttgc cgtcggcatt ggcggcgatg
    14401 attcccgcga catggctgcc atggccgctc cggtcttcca gcgtgctgcg attcccggtc
    14461 acgatgtcga tgctgttcgc ggtgtcgata cgccctgcaa actcggagtg accgagggaa
    14521 attcccgaat cgataacggc gaccttgacc ccgttgccgc gggcgccggc gctgtacgcg
    14581 gaatctgcgc cgatctgcgc aatgccgtag ttggccgcgg tctcggcggc gttggtgccg
    14641 ggcaccgggg gcgggacgac aggcggctgg gccggcacca cgggtggtgt cgagacgacc
    14701 gggccggcac tggatccgcc gcccgcaccg ccaccgccgc cgctacaagc ggtgagaagc
    14761 agaacggcac cggtcagggc cttgcagcga agcgaaggtc gatgccgcgc tccctctgga
    14821 gcaacctggc ttgtccggcc tcctggttga cgtgtcccgc tccccatgtc gtttccctgt
    14881 gaggcttcct cgaggatgat agttaacggg aaacccgtta cagtaaagcg ccacgtgggg
    14941 ttgcggcgca gtatgttatt cacactggga cccgtcggga ccggtccgat cggtgcccga
    15001 ggctcagtgg cgaacgtcga tgacgagctt gcctgccgcc tgacggcggc gcagtcgctc
    15061 gatggcctcg gccgtacgtg ccaggggcag aacttccgag acctgcggat gaatgcggcc
    15121 ggcttcatgc cactcgaaca accggcgaaa gccggcgcgc agccgctccg ggtcgcgctt
    15181 cctgtagctg ccccaataga aaccgagggc tgctgcgttc ttgaccagga ggatattggc
    15241 cgggatctgg ggaatagtgc ccgaggcgaa gccgatcacc acgatccggc cttcccaggc
    15301 gatggcgcgc agcgaggccg cgaagagcgc gccgccgacc ggatcgaaaa ccacgtcgac
    15361 accggcaccg ttggtcaagg cgcggatctg ctcgacgaga tccggggcgt cgctgtcgat
    15421 gacatggtcg gcaccatgtg cggtggcgat cgccagccgg tccgacccgc gagcggtagc
    15481 aattaccgtg gcacccatcg ccttgccgca ctcgacggcg gtcgagccca cgccgcctgc
    15541 ggcgccatgc accagcagca cctcgccctt ggccagcccg gcacgccaga cgagtgcgcc
    15601 gaaggccgtg ccataggcga tggcgaagcc ggcggcgatg atcggatcga agccttcggg
    15661 cagcgagacc acgtcgtccg cccgcgctat ggcctgctcg gcgaagccgc catggtcgac
    15721 gagggccagg acatgctcgc cgcattccag gcccttgacc ccgacgccga ccttgtcgat
    15781 gcggccggcg acctcgagac ccggcacgaa gggcagggct ggccgctcct ggtagcgacc
    15841 ctcgagcatg agggcatcag cgaagttcac gccggcggcg tcgacggcga tccggacctg
    15901 gcaggcacca gtctccggtg ccggcagctc tgccagctcc agaccggcag ggcccttgag
    15961 ctcccgagtg atgacggcgc gcatatcggc tctcgatcgg cttctcggtg tggatcggct
    16021 ggacggccgc ggccgggcac cgccaggcac gcgcggctgg cacaaacgcc attcctcgcc
    16081 tatctgcaag cccatgcgga acgatctcgc tcgcggctat ttcgccatcg gcgccgatgg
    16141 catctccaag cccatgaatc tcggcaacct cgtccgttcc gcgaacgcgt tcggtgccag
    16201 cttcgtgttt ctcgtcggcg tccgacagtc cgtgcggcaa atgaagtcgg acacctcgca
    16261 tgccgaactg caggtgccgg tctatgacgt cccggacgtc gccgcgttga gcctgccacg
    16321 gggctgccgg ctggtcggcg tcgagctggt cgaccacgcc gtggccctgc cgagcttccg
    16381 ccatccgctg caggccgcgt acgtcttcgg acccgagcgc ggcagcctga gcgagggcat
    16441 gctggcacgc tgcgatcacg tggtccgcat cccgacccgg ttctgcgtca atgtcgcggt
    16501 cgccggcgcc atcgtcatgt atgaccggat ggtgtcgctc ggccgcttcg ccgagcggcc
    16561 cgtgcgttcc ggcgggccac cggcagggcc cgggacaccg gaaagccgat agacgctatg
    16621 cacttccgtg aacgggctgc gcgagctatg tagcatgcgg agttctgctc aacgaccgca
    16681 agggagccca tgtccaggca attcgtgatc gtcgccggtc tcctggcgct gctttcgctc
    16741 ggtggcgagg cctttgccca atccccgcaa ttcctgggca attttcgcga ctggtacctc
    16801 tatgcctacg acgattccgg cggcaagacc tgctacatcg cgagcaagcc gaccgaggag
    16861 gagggcaact accgccgccg gggggccgcc gccgtgctgg tcgcccgcct gccgatcgcc
    16921 ggtgccggag agcaggtgag cgtccagccc ggctacagct tcaagccggg cagcacggtc
    16981 gagatccgca tcggcggcaa gcgctgggac ttcttcaccc agggcgagca tgcctgggcg
    17041 aacaccgacg acgaggacag gcaggtgatc gcggccatga aggacggcag cgacatgacg
    17101 gtgcgcggga cgtccacgct cgacaccttc tcgctcgaca cctactcgct gctcggcttc
    17161 accgccgccc tcgaggccct gcgcgatgcc tgccccggct gatcccctgc cagtcgccgg
    17221 caccaccgct gcaccggcac cgaagccgct ggcggcggac ggccggccga gcctgatcgg
    17281 cctcgacctc gcctcgctga ccgaggtgat cggggcgttg caggtggtgc ccgagcggca
    17341 ggcggcgctg cgggcacgcc agctcttcca ttggctctac catcggggtg cttgcgactt
    17401 cgcctcgatg tccaccctgc ccaagccatt gcggcaggcg ctggacgatg gctggtcggt
    17461 cgcccggccc gacatcgcga ccgcgcagac ggcggccgat ggcacccgca agtggctcct
    17521 gcgcttcgcc gacggccagg aggtcgagac cgtcttcatt cccgaggacg accggggtgc
    17581 gctctgcatc tcgacccagg tcggctgcac cctgacctgc tcgttctgcc ataccggcac
    17641 catgccgctg gtgcgcaatc tcggcgtcgg cgagatcacc gcgcagctcc tcgtggcacg
    17701 cgacgcgctg ggcgagtggc cgacggcgaa ggagggacgg ctcttcacca acatcgtcgt
    17761 gatgggcatg ggcgagccgc tcttcaacta cgacaacatc gccggtgccc tgcgcctcgc
    17821 catggacgag acgggcctcg cgatctcccg ccgccgcatc acgctctcca cctcaggcgt
    17881 cgtgccccgc atcgcccagt gcggccggga gctcgaggtc gggcttgcgg tgtcgctgca
    17941 tgcggtgcgc gacgagctgc gcgacgagct ggtacccctg aaccggaaat ggaacatcgc
    18001 cgagctcctg gaagcctgtc ggcagtaccc ggcgcggcgg ccgatcactt tcgagtatgt
    18061 catgctgcac ggcgtcaacg acagcgacgc cgatgcccgg gagctggtcc gcctgctcgc
    18121 cggcctctcc gccaaggtca acctgatccc cttcaacccc tggcccgggg caccctatgc
    18181 gtgctcctcg aacaaccgga tccgggcctt cgccgccatc gtcgaggcgg ccggctatgc
    18241 ctcgccggtg cgcacgccgc gcggccgcga catcctcgcc gcctgcgggc agctgaagac
    18301 ggcgagcgag cggcagcgcc ggcagcgggt cgccgcttga tcgtcgcgcg ctccccgcgg
    18361 caccatccgt gaagcgcctg atcgtcggat tcctggcggt tgtcggggct ctggcgctcg
    18421 tcgtgctggt ggccgccata gcaggcggca tctggttcac ccggcagttc acggccgggc
    18481 ccgagctgcc cgagcgcatc gtcctgacgc tcgacctggg cgagcctctg gtcgagacgg
    18541 tgccaggcga cccgctggcg acgatcggct tggagcagcc gctcgagctc ggcgagatcg
    18601 tgctcgccct cgagcgtgct gccgccgacc cccgggtgcg cggcctcgtc gcccggctcg
    18661 acgccaccga ccacggcatg gccgtcgccc aggagctgcg ggcgacgatc gaccggttcc
    18721 gcgccggggg gcgcttcgcc cgggcctggg ccgacagctt cggcgagctc gggccaggca
    18781 acgagggcta ctacatcgcc acggccttcg acgagatcca cttgcagccg ggcggcatgg
    18841 tcggcctgac cgggctcctg gcggagattc cgttcgtccg cccgctgctc gaccggctcg
    18901 gcatccaggc cgaggtgatc cgccggtccg actacaagac cgccttcgac agcctggtgg
    18961 aggacggcct caccgccgcc catgccgaga tgatgaacga cctgctcgac cagatctatg
    19021 ccgcgctggt cgacggcgtt gccgcctcgc gcggccttgc gcctgccgag gtcgagcggc
    19081 tgatcgatgc cgggcccttc accggcgagg cggcgctcgc ggccgggctc gtcgacggcc
    19141 tcgccttccg tgacgaggtg ctcgatcggg ccctcgcgga ggccggcccg ggcagcgcgg
    19201 cggtcggcct cgccgcctat gccagggccg agccggagag cgacggcgag ccgcccgcca
    19261 cggtggcgct catcgtggcg caggggacga tccagcgcgg cacggccggc ttcgacgcct
    19321 tcatcggcgc ggacgacctg gcggcacagc tcgccgaggc gcgcgacgac gacgccatcg
    19381 acgcggtggt gctgcgtctc gacaccggcg gcggctcggc ggtcgcgtcg gagaccatcg
    19441 gccgcgaggt cgagctcctg cgccaggccg gcaagccgat cgtcgtctcg atgggcaatg
    19501 ccgcggcctc gggcggctac tggatcgcga tgggggcctc gcacatcgtg gcggagccgg
    19561 cgacgctcac cggctccatc ggcgtgatcg ccggcaagcc cgtgctggcc gggctctggg
    19621 agcagctcgg cgtcaactgg gcgaccctgc agcgcggcga gaacgccggc tacctgtcgc
    19681 tgaaccggcc cttcgacgcg ctggcgcgcg agcgcctgga gcgcgcggtg ggcgccctct
    19741 acgagcgctt caaggccggc gtcgccgagg ggcgcggcct cccgccgaat tcggtcgagg
    19801 cgctggccca gggccgcgtc tggctcggat ccgaggccgt cgggctcggg ctggtcgacg
    19861 agctgggcgg catcctcgag gcgcgcgcgg ccgcggcacg cctgctcgag ctgccggccg
    19921 gcagcatccc gcatctccag cgctaccccg agccaccctc gaccttcgag cggctgcgcg
    19981 agctcttcga ccagccatcc ctcccgggcc tcgcgtccct gatcgccctc tggcacgggg
    20041 cgctcctgcc cggcaccgcc gagatcacgc tgccgcgctt ccgctgattc ctttcataat
    20101 aggatttata gcctatgagc gccgcggggt caagcgcgcc tgcgggtttg tccggcaaaa
    20161 cagggtcggc cggctcggat tcgagcggaa aggcagattt ccggcggccc gggggtcgcg
    20221 aacgaacccg cgagaccgac gaacgaaccc gggaaacgcc actttcggcg ggttcgttcg
    20281 tcgattcctc cggttcgctc gtcgagtgct gtcgcggaat ctgcgacatc tggcccgagc
    20341 tgtcgcggaa tctgcgacat cacgaatgct tatgtcgcag aatttgcgac agtcgggcct
    20401 cgcctgtcgc ggattccgcg acaacccggg cggtggtgtc gcagattccg cgacagtttg
    20461 ccgggcggtg ggatcgtttg cgggcctggc ggcggccctc gactgttcgg gcgtcgccgg
    20521 tcataacgat tcgtgatagt gcccggggcg acgggaaggg gcgttgcggg ctacgccctg
    20581 aggttaaagt ggtctcgtga ggcagttgca aaactccgga tgaactttgc tttgccgtca
    20641 cggaatattc tttggtttga gcaccaattg acgccggcct gtcggcatgg cggtgggaat
    20701 tcaggcggga gtacggtttt cgcgaatttc gggcgagcgg aagccggcat cgagttggtc
    20761 gaggaccatg gctttgactt gtgtgcgggc ggtcaggtga ccagtcggat gagctggtcg
    20821 acggtcagtg ccaggatgcc gaacatcagg tggcacatgg ccttggtcgg accgcggacg
    20881 cggacatggt tgccgccgaa ctgatccttg agccggctgt tgacgcgctc gcaggaggtt
    20941 cgctgcgcca gccgcctggc ttcggcgggc ttccaggcga tctgctcacg ggcttttttc
    21001 tcgttggccc aggcgtcttt ggccgccttg ctgcggggga ccgggtcgat gaccgggaca
    21061 tggccgaggc tgcggctgtg gtcgtggatc tcccgcacat cgtaggcgct gtccatcagg
    21121 tcgtagaggt tggtgacgcg ccgcgccgtc atcaccgcca gcgggatggc ggcctgactg
    21181 tcgtggacat gcgcgccggt gagcaggcag ctgaccggga tgtcgccgtc ggccacatcg
    21241 atatgcaggc ggtagccgcg ccagaattcc ttatagccct tggcgttctt gcggttgccg
    21301 atggagcagg cgcgcggcag gtcggcgagc ctcgcctcga ggctcatgcc gggctgcgtc
    21361 gccagccggc gcacgggttt ctcgacgatc tcgcccttgc gtggccggcc gcgtccccga
    21421 cgcttttccg gctgcttcgg ggcagcggcc aggggcggcc gctcgcgcgc cgcgatcgcc
    21481 gtgccgtcgc gtgagacgtg gccgatcagg cagccaccca gccgctcctc gaccagcgcc
//
LOCUS       DAOTFI010000056        21355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2954_length_21355_cov_6.639531, whole genome shotgun sequence.
ACCESSION   DAOTFI010000056 DAOTFI010000000
VERSION     DAOTFI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21355
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2954_length_21355_cov_6.639531"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            826..1368
                     /locus_tag="P5558_07555"
     CDS             826..1368
                     /locus_tag="P5558_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24201.1"
                     /translation="MMGALWPTLRALQIFAVASEMGWQSKILLFLLITCVSTSALGDP
                     GRFSEGYRHEFERFAQVPPRPLIDEVRSRLGYVLAGHGFMQLDDTEAVLAMESRLWRS
                     IIDRCCQVAKFSACLADLPRIDHMLAEEALGYASEIAQSPDPLLLGSATGLLALFAAR
                     AGPECACRYGPGEALSACGF"
     gene            1356..3008
                     /locus_tag="P5558_07560"
     CDS             1356..3008
                     /locus_tag="P5558_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRY24202.1"
                     /translation="MRVLSAAVFLILGLFGAISAGTEDASEPLTPEAAAAQLATSGVL
                     HNVSVAGTLDLSDLTPEPGIRRLRLEGVRIVGYLQGPAPLPLEIVGGKACGLGIARDA
                     QAWPAPISIQDALIGSLHLSKIRLGKFTCAGCRIMNGRFKGTRFAGPIRADLKIKQHD
                     GLAGLCPELRTGGGLDVVDLDVSGGAVFEGDVDFRGLSAEAAVTFRAARFEGDTSFVG
                     VRLAGRADFSGAQFRKSAEFRNCDIGTLEIDRGQSALLATAFDGPADFRNCNITETRL
                     DFVTLAKSADFRLSRLARVEVGTISGPQRSLDLRTLQDVEYLRLTPAAVPIVDFYWHE
                     IEGALTLDDTSGALERAIVDAVRANGTPLDARRASYALKAAAWQRLVDGLTGDGPMPE
                     DREREIARTLEWGLWSWPTKEGTSLVRLLWLGLVLWALASLALLVHRRSLVRLPARGE
                     PGLDAIRPWHEIVVADLPEGALCPLGGLRQASLTLAYAFQLCFLWSPGNLRHAKTTGD
                     LHQSDVTYLRLLLWLCGKYLVLLAALTLAALTPALQGLLPMG"
     gene            3019..4128
                     /locus_tag="P5558_07565"
     CDS             3019..4128
                     /locus_tag="P5558_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24203.1"
                     /translation="MAALASLAGPAAAADGILARLAALGALDDTYVHVIQDVAADLAP
                     LMGGVPRFTVNTEPHGAEIGAVVVDTALAVRDAAISGAVWLKAAERGAYTIEEERLIV
                     IGGDVLRRFAAASQLWMDGREGIDIGLILQAWSLAELEDLWSPQRNTALARDDGYLGT
                     YEIFAGMLAFLLAHETYHILTPPQRPEQLPQDAPLWGRAKQLAWGCSALVGGIANAQR
                     AYESRADAFAVSLLARIPDSAQGATARLKYEYGTYAAGICFMGQEMVDFAARTSDPKI
                     GTIVATMISADLRPLARRFETGTIPLEELVPTIFAATHPSVVDRLIVVTKDLRSQPGS
                     AFYGEAGMEGASDLMLWNMIRESICEEARKAGALE"
     gene            complement(5143..6366)
                     /locus_tag="P5558_07570"
     CDS             complement(5143..6366)
                     /locus_tag="P5558_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRY24204.1"
                     /translation="MATQTKALIAQGGGPTAVINQSLVGAVLECRKFPQIDRIYGALH
                     GVRGIVDEDLLDLTQETTNNLELVAGTPSSALGSTRDKPDRAYCKAIFETLQAHEISY
                     FLYIGGNDSSDTVRIVAEEAKAAGYPLRSIHIPKTIDNDIVLNDHTPGFPSAARFIAQ
                     AFMGINLDNKALPGVYLAVVMGRNAGFLTAASALGKKFPDDGPHLIHMPERAFDPNAF
                     LAGVKATFDRHGRCIVAVSEGIHDTEGKPILARLAGELQRDAHGNVNLSGTGALADGL
                     ATLVKDRLAIKRVRADTFGYMQRSFVGCVSDVDQREAREVGEKAVKFALWGDRDGSVT
                     IQRVGNYQVDYELVDLDQVAAKTRTMPDAFVDAAGTGVTDAFHMYLRPLLGSGMTNEY
                     RLQTSNRVAKLRRPG"
     gene            complement(6532..7044)
                     /locus_tag="P5558_07575"
     CDS             complement(6532..7044)
                     /locus_tag="P5558_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24205.1"
                     /translation="MAATLLLGLLLALPAVAAPAVFGTLDRLALDIRGAMAHVARLRD
                     RLDEGGFGDASSLSPAAEVAGIADGRFGSTVASLRWASRGLDRRLALLRATVGTPDSP
                     EKAEILLVMRVELSSLLWTLEELGGSAAETPQAPTATERALALDQLNASLEQLAIATS
                     AMAGFDWGSF"
     gene            7281..7565
                     /gene="rpmB"
                     /locus_tag="P5558_07580"
     CDS             7281..7565
                     /gene="rpmB"
                     /locus_tag="P5558_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRY24206.1"
                     /translation="MARRCSLTGKGVQAGNNVSHANNKTRRRFLPNIQERRLFSEALG
                     RSVRLRIATNTLRTIEKRGGLDAFLLSSSQTDLPSDLGRIRKLIAASNAG"
     gene            7600..8121
                     /locus_tag="P5558_07585"
     CDS             7600..8121
                     /locus_tag="P5558_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HRY24207.1"
                     /translation="MIAAAGLAMTVIVVASNILVQFPVNDWLTWGAFTYPVAFLVTDL
                     ANRRLGPGPARRVVYLGFALAVLSSIVLATPRIALASGTAFLMAQLLDVSLFHRLRRA
                     TWWRAPLVSSAVASTLDTALFFTLAFAGTGLPWIGWGIGDLGVKLAVALAMLVPFRVL
                     MGRQDALPKHLPA"
     gene            complement(8126..9184)
                     /locus_tag="P5558_07590"
     CDS             complement(8126..9184)
                     /locus_tag="P5558_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRY24208.1"
                     /translation="MGHRSAWFDGPETGADAWAKGPAARPRRRRLAGLLALGWVLVAL
                     VGCVAESAIPSATPSVTISRLEVEPAGVRTAPGVELLLAFELTGDDPRFGGFSGIATD
                     GRSLWLLSDRAVLWQAGLGLEELPRNLRLDGWRAGSIHRDTADRAALDSEALALDADG
                     GLVVGFEQDGSLRRLEADGAGGWTTRRLHDGSLTGDSPANRGLEAATGLGDGSFLVLS
                     EGGRQAADIARGSRLGPDGSTPVTYGTARGFSPVGAAIAGDRLLVLERSVGVLTGWQT
                     RVTVIGRQEWASAGPMAGPMVGEELLRIGAGPLAENYEGITSLDLGDGSRLLLLVADD
                     NQSPLQRTLLLVFSWRGS"
     gene            9176..9817
                     /locus_tag="P5558_07595"
     CDS             9176..9817
                     /locus_tag="P5558_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24209.1"
                     /translation="MSHALDLALVYALVVQSAHLLLAAGGGWATLRGLVLERSAQGWI
                     ALAAAIGMALATGAVTGALAPAALTWLKLAGGAALLATVAWVELTPRGPFWRAMAGNA
                     PGLVRAGIAPRPGLPLALGLCLAALAGVAAPAPPTAANWLPLACLALLWLAGTDHRPG
                     LMVRRAVLAFLAGALVGGLALLLPPQPHALVVGFLTLAVVGLALWRPSTAVAN"
     gene            9826..10764
                     /locus_tag="P5558_07600"
     CDS             9826..10764
                     /locus_tag="P5558_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24210.1"
                     /translation="MGAQAIALAPYLVLPAVLLALGLPQATAFTGFTAGLVLLVLAAH
                     IAVGVLRLPFLCLGGLAAAGAAFVQALSGLAGLEPWAALAMVPALGLALGGAFGLVLR
                     WLPGDGEETGTALLSLALLLPLAALPLAAGGTRGDGVAVEDAIVLVPLLSLLAVLARL
                     FAGSRLAELAAAAAAGGLRAEAAGLDLAGWRITGLCLAAAIATTAGAVMLIGPAPAAS
                     ASMEAWAALAVALFAIGRLGGARFGGCLLAALPLALLPRLTVTLSPAFGDLTLAMALA
                     ALLLQAVVRRDGSLALLARPARPRSAAPFAAARQEP"
     gene            10772..11548
                     /locus_tag="P5558_07605"
     CDS             10772..11548
                     /locus_tag="P5558_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24211.1"
                     /translation="MSDLALDQPLHPLLELVGVAVHRPGLPPLEPLTCAFDAARTTLV
                     VGIEGSGRATLCDLLAGRLLPDRGVMRLDGQRIDRLPAAARVRLGIVTCGPERLVQHG
                     SVRAMMLTARLAVTRPVWSLAFGLGRPSRQDREDIEELLGFVGLDHLAARGVPTLGGL
                     EARLADLARCLAMRPRVLVVGHGLAGLEAPDRKAFAALLQRVAAAGPAIVAIDDDLAT
                     LGAYADLCLVLHRGRLLANATPRAIGEDSRVFEALTGAEL"
     gene            11545..12270
                     /locus_tag="P5558_07610"
     CDS             11545..12270
                     /locus_tag="P5558_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24212.1"
                     /translation="MSAALLRLEALDIVAGGAWLLEGISLEVVEGSTVALVGGPGAGK
                     SLIVDLVLGLASPRAGTVRLDGRNITALPPLARQRSGIRAAFAEPPVFPGLTIREHLA
                     LAVAGGRLRARALSRLAEYLPELEGRLDQPVGSQGAAVLRLVDLARAMLGMPRILLID
                     DLFPALGAERAGELVRSLARDGYTLLLADRYGEAVLDHADHGYVLAQGTIVAEGAPAT
                     LRADPRLLATCAGDCSAWAEIPA"
     gene            complement(12346..13047)
                     /locus_tag="P5558_07615"
     CDS             complement(12346..13047)
                     /locus_tag="P5558_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRY24213.1"
                     /translation="MDQRLAHGAVVDTSIDAGLRSHMLSVYNYMGLALVLTGLTAFVT
                     ASSPALLQVIFGTPLKWVVMLAPLGFIFFLGAKIRTMSVSAAQITFWSFAVVMGLSLA
                     SIFLVFTGASIARVFFITAATFGAMSLYGYTTKRDLSKYGSFLFMGLIGIVIAMLVNI
                     FIGSTALQFAISVIGVLVFTGLTAYDSQRIKEEYYSFNSAEIATKGAIMGALSLYLNF
                     INLFMMLLQLFGNRN"
     gene            complement(13231..15768)
                     /locus_tag="P5558_07620"
     CDS             complement(13231..15768)
                     /locus_tag="P5558_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24214.1"
                     /translation="MRAGAGGAWNLGALWRIAWRDLRGSSTAALIILGCLTLGVATIT
                     GIGSLRASLTDTLERDARALLGGDLVIESTYRELPPEELRAIVPAGATVAHTVTTNAI
                     AFADNGRQVPVALKSIDGAYPLYGVVGIEPLMTLEDALADGGALVEAALLQRLAIAVG
                     DSLALGNGRVTVRGVLTAEPDQIGGFVGLGPRVMIDRATLDALDILAPGALVRHKYGL
                     ALADRSEAGAYVATLRRDFPEAAWRAQTFNDVQPRIARNADRLAGYLSLAGIAALLVG
                     GLGVGIVVDAHLQSRRRSIATFRCVGMTTRDVFLVCGLQVLLLCCGGLVLGAALGTAL
                     PFGVHLLPAGLIPIDLAIGVQPGAILAAALFASATAALFTVLPLLKAVSVTPAALFRD
                     DALAAGPRPLRRQAWPWLLGLGLVLVLTALLAVPAPHVAVIFLGIVLVAALAMAVLAR
                     LGLEGAGWLARHTTGMWRLALGQLRRAERMAVASVVALAAGTAVLTTVLLVENGLSRE
                     LLENRPMNAPSTIFIDIQGTQRAEFEAVVGAFPDASVLQMEPVLRARVVKIAGVPVDE
                     AVIAEGARWTVRRDRGLSYRAAPPEHTTLVAGEWWPADYAGPPLVSMEDDIALGYGIG
                     VGDTVTFNVLGRLIEAEVANLRPEIDWSEGRIDFVFVLSPGVIDQAPHTLVATVDVPK
                     GEQAALLDRMARELPNVTPFAIDELIAKVADILDKIGLAVRVMAAVTLVTGLAVLTSA
                     LVAARREQLRQAVLLKVVGAERRQIGGIFLGQYAAIGVVASLLGALLGLVASFAVLRF
                     AFGLPWHPSALPVTVVPVVAVLATLLLGALGLRHILAVPAALILRER"
     gene            complement(15765..16463)
                     /locus_tag="P5558_07625"
     CDS             complement(15765..16463)
                     /locus_tag="P5558_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24215.1"
                     /translation="MICLRDVHLTLRSSAGPVDILNGIDLDVAAGTSLSVVGPSGSGK
                     SSLLAIIGGIERPTRGRVEVAGTDLGTLDEDGLALFRRKRVGVLFQSFHLIPTMTAQE
                     NVALPLELAGEPDGFERARAGLAEMGLGQRLDHYPGQLSGGEQQRVALARALANEPEL
                     LLADEPTGNLDRSTGRQIAELIFAARERHGATLFLITHDPTLASLCDETRAMRDGRLD
                     AGDVAADAFARASS"
     gene            16462..17190
                     /locus_tag="P5558_07630"
     CDS             16462..17190
                     /locus_tag="P5558_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRY24216.1"
                     /translation="MSTGPSTNYRAATYRAIDGQRPLQLGTRRRILKRAAALCLIAAS
                     PLATRRAGAAGPEPCRIAILGDSLTAGYGVALEDAFPVRLEAALREAGHACEIIDAGV
                     SGDTSAGGRARLDWVLGDDPTHLLVELGGNDALRALPVDAMEANLAAIIEGAQARGVS
                     VMLLGMLAPPNLGSRYTEAFAAVFPRLAERYAVPLYPFFLDGVAADPAYLTEDGIHPN
                     AAGVARIVERLLPSVEIWLDESAD"
     gene            17550..19481
                     /gene="acs"
                     /locus_tag="P5558_07635"
     CDS             17550..19481
                     /gene="acs"
                     /locus_tag="P5558_07635"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRY24217.1"
                     /translation="MSDLYEVSAEWAASAHCDAPTYRRMYEESVNDPETFWGREAGRL
                     SWAKPPTRIKRTTYSGDVSIRWFEDGELNVCYNCVDRHLPARANQVAIIWEGDNPDVD
                     LKITYRDLHRNVCKVANILKDMGVKKGDRVTIYLPMIPEAAYTMLACARLGAIHSVVF
                     GGFSPDSLADRINDCGSKLVVTADGGLRGGRSVPLKGNADKALAKCADDVKLLMFRHT
                     GIDVPMKAGRDVDGMLAMKEASDECGYTVMNAEDPLFILYTSGSTGKPKGVLHTSGGY
                     LLFAAMTHQYVFDYQEGDVYWCTADVGWVTGHSYILYGPLANGATTLMFEGVPNYPDM
                     SRFWNVVDKHAVSIFYTAPTAIRALMQKGDDPVKATSRKSLRLLGSVGEPINPEAWRW
                     YHTVVGEGRCPIVDTWWQTETGGIMITPLPGAIAQKPGSATLPFFGVQPALVDNDGKP
                     IEGAGEGNLVIKDSWPGQMRTVYGDHERFIQTYFTTFKGVYVTGDGARRDEDGYYWIT
                     GRVDDVINVSGHRMGTAEVESSLVAHPAVAEAAVVGFPHDIKGQGIYCYVTLQNGIEP
                     SEALEKDLVRHVRNDIGPIASPDHIQWAPGLPKTRSGKIMRRILRKIAANEHDALGDT
                     STLADPAVVDDLVANRKGA"
     gene            19551..20048
                     /locus_tag="P5558_07640"
     CDS             19551..20048
                     /locus_tag="P5558_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24218.1"
                     /translation="MKERLEYAGLAFQTTSLCVGILYVTGFYINSLFTSNFGIFRTEL
                     LRLDYIKVGFVFNLITLGIVFVPIGIVLLSFSVRSSSNLPNFYLGIIGNSLNTILFFG
                     VPIFFAFFATSVEWNLAFVEPLYIFTTVESAVITFLVITFFGMAVIPALERLLYGTNF
                     FAALL"
     gene            20081..20638
                     /locus_tag="P5558_07645"
     CDS             20081..20638
                     /locus_tag="P5558_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24219.1"
                     /translation="MSFGLALAIAIKIFSAIEWIPALLRGGLYYLLTAIVFVLGMVAA
                     GFWIRKIRHVKFAGLVYVLVAFGLAILFYLSVTSYVFGVYKFIPKNRGGGLPVTEIYF
                     DSSNHKMLLSELASFSPIIGFGPFYIVEENSDFLFLASAEMEKWTIDYVPIFVMRKSH
                     LDHIFIRRIPSGFPRTISSASSSRP"
     gene            complement(20807..21175)
                     /gene="tnpA"
                     /locus_tag="P5558_07650"
     CDS             complement(20807..21175)
                     /gene="tnpA"
                     /locus_tag="P5558_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRY24220.1"
                     /translation="MRWAAVPAEVKKSYPSKSQAKKSPDPFSAPRRTADGHRQLHHVQ
                     GHDLFLAIPPRHSVSDVMRRIKGRSSRKIQQEFPSIRKRYWGCHFWARGYFSTTAGNI
                     TQYTILQYLADHIEPTGVSR"
BASE COUNT         3333 a         7436 c         6946 g         3640 t
ORIGIN      
        1 tcactgtccc gctctcgtta tagctgtttt gacatgattc ggcaacggga gaaagtcgca
       61 acgttcggcc gcaggccgac ccaaagtccg gcttcggccc ggcgcctcga tcggttacgt
      121 gtccacttaa ccttcttgag tgacgaatcg ctgaagcgtt tccgacggcc ttcggccctg
      181 gttacggtag gcgcgggcat ctcgggggtc acggcgcgtc gttcctaatt tgatctcgat
      241 caagactccc aagcgtaatt atggtaggat tctgtgtccg aatggtaagg gggtagattg
      301 accttcgtct catgaaggca aaggaggatg ttatgcgcgt cgtatccaca agtctagcgc
      361 ttgttctcct catcgctgga gctccggagg tcgggcgcgc tcagagtcaa acgtgcaacc
      421 ccgatgaagt cggccagatc aggggttttc ttgagcgatg gccatatgaa aaacaatata
      481 tgataggttt cacgttagat ttccaaagtc tgcaaatgtt aatggatgaa ctaaattccc
      541 tagccgcttc gctttctccg gattgtcaat tgttcttgat gcggttgaat caaacaggcc
      601 agacacgccc acagacagcc ccaaccttag gcggtggcgg cagcgtgttg tacgacgagg
      661 gctccgacac cttttatggc cctggtgtcg cttgcccgcc ctccggctgt attccttata
      721 actgactaag gatggccagt aggcgactat tttggcgaat caagaatctg ggaatgcttc
      781 ttgcgaacac gcaatctagg aagacgttgt cgccggacac ggaagttgat gggggcgctg
      841 tggccgactc tgcgggcttt gcagattttc gcggttgcaa gtgaaatggg atggcaatcc
      901 aaaatcttgc tttttctgct gatcacctgc gtcagcacat cggcgctcgg cgatcccggt
      961 cgcttctccg aaggttatcg gcacgaattt gaacgcttcg cgcaagtgcc tccgcgaccg
     1021 ctcatcgacg aggtgcgctc gcggctgggc tatgtccttg ccgggcatgg atttatgcag
     1081 ctcgacgata ccgaggctgt tttggcgatg gaatcaagac tctggcgttc aatcatcgat
     1141 cgctgctgcc aagtggccaa gttcagtgcc tgcctcgctg atctaccccg gatcgatcac
     1201 atgttggccg aagaggcgct cggctatgcc tccgagatcg cccagtcgcc ggacccactc
     1261 ctccttggtt cggcgaccgg attactcgcg ctgttcgccg cacgggccgg acccgaatgc
     1321 gcctgtcgct acgggcccgg cgaggcgctt tccgcatgcg ggttctaagc gcagccgtgt
     1381 ttcttatcct cggcctcttc ggagcaatat ccgcgggcac cgaggacgcc tccgagccgt
     1441 tgacccccga ggcagccgcc gcgcagctcg ccacctccgg cgtgctgcac aacgtgtccg
     1501 ttgccggcac gctcgacctc tccgacctca cgcccgagcc cgggatcaga cggctgcggc
     1561 tcgaaggggt ccgtattgtc ggctatctgc aaggccctgc gccgctgccg cttgagatcg
     1621 ttggcgggaa ggcctgcggg ctcgggatcg cgagggacgc acaggcttgg ccggcgccca
     1681 tctcgatcca ggatgccctg atcggcagtc tacatctctc gaaaatcagg ctcggaaaat
     1741 tcacctgtgc ggggtgccgg atcatgaacg gccgcttcaa gggcacgcgc ttcgccgggc
     1801 caatccgggc ggatctcaag atcaagcagc atgacggcct cgcgggcctt tgccccgagt
     1861 tgcgcactgg cggcggactc gatgtcgtcg atctagatgt atctggcggc gcagtcttcg
     1921 aaggcgacgt cgattttcga gggctctcgg cggaggctgc agtcaccttc cgcgccgctc
     1981 ggttcgaggg tgataccagt ttcgtcggcg tccgactcgc cggccgcgcc gacttctccg
     2041 gcgcccagtt ccgcaagtcg gccgagttcc ggaactgtga tatcgggacg cttgagatcg
     2101 accgcgggca gagcgcgctg ctcgcgaccg cattcgatgg gccggcggat ttccggaact
     2161 gtaacattac cgaaactcgg ctggattttg tcaccctcgc caagtcagct gatttccggc
     2221 tgtctcgcct agcgcgggtg gaggtcggca cgatttcggg tccgcagcga agcctcgacc
     2281 tccgcaccct tcaggacgtg gaatacctga ggctcacgcc cgccgcggtg ccgatcgtgg
     2341 acttctactg gcatgagatc gagggcgcgc tgacgttgga cgacacatcc ggagcgctcg
     2401 aacgcgcgat cgtcgatgcg gtccgagcca atggcacccc gctcgacgcg cgacgggcca
     2461 gctatgcgct gaaagccgcg gcttggcaac gcctcgtcga cggcctcacg ggcgacggac
     2521 ctatgcccga agaccgggaa cgggagatcg cgcgcacgct cgaatggggg ctgtggtcgt
     2581 ggccgaccaa ggaagggacg agcctcgtcc gcctgctttg gctcggcttg gtgctctggg
     2641 ctctggccag ccttgcgctg ctcgttcacc ggcggagcct cgtgcggctg cctgcccggg
     2701 gcgaacccgg gctggacgcg atccggccct ggcacgagat cgtcgtggcc gacctgcccg
     2761 agggcgcgct ctgtccactc ggcgggctgc gccaggcaag tctgacgctc gcttacgcat
     2821 tccagctgtg ctttctctgg tcaccgggaa acctccgcca tgctaagaca acaggcgatc
     2881 tgcaccaatc cgatgtcacc tacctgcgac tgctgctgtg gctctgcggg aaatatctcg
     2941 tactcctcgc cgcgctcacg ttggccgcgc tcacacccgc gctgcagggc cttctgccga
     3001 tgggctagga cgctggccat ggcggctctg gccagtctcg ccggtccagc tgccgcggcg
     3061 gacgggatct tggcgcggct ggccgcgctc ggcgctctgg acgacaccta tgtacatgtt
     3121 atccaggatg tagcggccga tctcgccccg ctcatgggcg gcgtgccacg ctttaccgtc
     3181 aacaccgagc cccacggagc cgaaattggt gcggtcgtcg tcgacaccgc gctagctgtg
     3241 cgggacgcgg cgatatccgg ggccgtttgg ctaaaggcgg ccgagcgcgg ggcctacacg
     3301 atcgaggaag agcggctgat tgtgatcggc ggcgatgttc tgcgccgctt cgctgcagcc
     3361 agccagttgt ggatggatgg ccgcgagggc attgatatcg gcttgatcct gcaggcttgg
     3421 tcgctggccg agctagaaga tctgtggtcg ccccaacgca acactgcgct ggcgcgtgat
     3481 gacggctatc tcggcaccta cgaaatcttc gcaggtatgc tcgctttcct gctcgcgcac
     3541 gaaacctatc atatcttgac cccgccgcag cgtcccgagc agctgccgca ggacgcgccg
     3601 ctgtgggggc gggcgaagca gctcgcctgg ggctgttcgg cgctcgtagg cgggattgcg
     3661 aacgcccagc gcgcctacga gtcgcgcgcc gacgccttcg ctgttagcct tctggcgcgc
     3721 atccctgatt ccgcgcaggg cgcgaccgcc cgcctcaagt atgagtatgg aacttatgcc
     3781 gctggtattt gtttcatggg gcaggaaatg gtggacttcg ccgcgcgcac cagcgatccg
     3841 aagatcggca ctatcgtcgc caccatgatc tcggcggacc tgcgaccgct cgcccgccgg
     3901 ttcgagactg ggacgatccc gctggaggaa ctggtaccca cgatcttcgc cgcgacccac
     3961 ccctccgtcg tcgaccggct aatcgtggtc accaaggacc tgcgcagcca gccgggcagc
     4021 gcattctacg gggaggcagg gatggagggg gcatcagacc tcatgctgtg gaacatgata
     4081 cgcgagtcga tttgcgagga ggcacgcaag gcgggtgcgc tggaataggc gctcgtttgc
     4141 tcaacaacgg cgacccggga cacctttctt ttcttgtaaa aaggcgggac acgaaccgac
     4201 tttaacaacc gtcgccgttc atcgagcggg gcttcgggcg acccgggagg ccgaccttct
     4261 cgacccgttt cgcgagcgcg gaggcaaggc atcgtcgatc gggctgcatc cccttgatca
     4321 tgccagtcag cccgcttcgc cttttccgag ccggctctcc agccgcagct caccgattcg
     4381 cacgaactcc cgcgtgctgg cggtgaccag gatgcggtcg agggtcaggt catgccggca
     4441 cgggacacga gatttcaata cgtcatggtg cggaccgcga tgggcacgac gtcgtccact
     4501 tcgccttcat cacgaacgct tatacctgcg tcccggagac ccgtctggcg gtgtcgctcg
     4561 gcgctctgcc gcccctgaat cgctcgggtt ggtgcatacg acgcgacact tcgccttggg
     4621 ttgtcgcgtc tcccgcaaca aagcctgccg ggatgttgca aattccgcga cataacgatt
     4681 cgtgatggcg catctgacgc gacattcgcc cggaaatgtt gcggattctg caacatgccg
     4741 ctgcgagcga acccgccgaa tcgacgagca aacccaagga atcgacgagc gaacccgggc
     4801 cttcgacgaa cgaacccgtg cgcccggtcc agcgattgcg ggcattcggc cgccgtttcg
     4861 ccgacgaggg aacccgaaaa gcgacccttg ccgatcggac ccgcgccctt gactcggcac
     4921 ccggaatcgg ctaatattcc tatatcggtt ggaagctctc cccccggctt taccgcgcca
     4981 cagagagcct cgggaatgtc gtttccaggg agctggtgtc gggcggccaa gccttcctgg
     5041 caaacgggat caaggccagc gagatcacgg gtgacatgct cgatcaagcc tatgccggcg
     5101 gcggcgtggg ccgggccgga ccggccgcga tggaccgccg gctcagcccg gccggcgcag
     5161 cttcgccacc cggttgctgg tttgcaggcg atactcgttg gtcatgccgg agccgagcag
     5221 cgggcgcagg tacatgtgga aggcgtcggt gacgccggtg ccggcggcgt cgacgaaggc
     5281 gtcgggcatg gtccgggtct tggccgccac ctggtcgagg tcgaccagct cgtagtcgac
     5341 ctggtagttg ccgacccgct ggatggtgac cgagccgtcg cggtcgcccc agagcgcgaa
     5401 cttcaccgcc ttctcgccca cctcgcgggc ctcgcgctgg tcgacatcgg agacgcagcc
     5461 gacgaaggag cgctgcatgt agccgaaggt gtcggcgcgc acccgcttga tggcgaggcg
     5521 gtccttgacc agggtggcga ggccgtcggc cagcgccccc gtgccggaga ggttgacgtt
     5581 gccgtgcgcg tcgcgctgca gctcgccggc cagccgggcg aggatcggct tgccttcggt
     5641 gtcgtgaatg ccttccgaga cggcgacgat gcagcgcccg tgccgatcga acgtggcctt
     5701 gacgccggcg aggaaggcgt tgggatcgaa ggcgcgctcg ggcatgtgga tgaggtgcgg
     5761 cccgtcgtcc gggaacttct tgccgagcgc cgaggcggcg gtgaggaagc ccgcgttgcg
     5821 gcccatgacg acggcgaggt agacgccggg cagcgccttg ttgtcgagat tgatgcccat
     5881 gaaggcctgg gcgatgaaac gcgccgccga ggggaagccc ggcgtgtggt cattcaggac
     5941 gatgtcgttg tcgatggtct tggggatgtg gatcgagcgc agcggatagc ccgccgcctt
     6001 cgcctcctcg gcgacgatcc gcaccgtgtc cgaggaatcg ttgccgccga tatagaggaa
     6061 atagctgatc tcgtgcgcct gcagcgtctc gaagatcgcc ttgcagtagg cgcggtccgg
     6121 cttgtcgcgg gtcgagccga gcgccgatga cggggtcccg gccaccagct ccaggttgtt
     6181 cgtggtctcc tgggtgagat cgaggagatc ctcgtcgaca atgccgcgca ccccgtgcag
     6241 cgccccgtag atccggtcga tctgcgggaa cttgcggcat tcgagcaccg cccccaccag
     6301 gctctggttg atgaccgccg tcgggccgcc gccctgggcg atgagggcct tcgtctgggt
     6361 tgccatgcgc gtcttccctg atcgacccgg cctccctgac ctgccggatc cgggcctgcc
     6421 tcgcgatacg ggcatggggc cacctgcggc acggcttgtc aatcggcagg acccgatcgg
     6481 tcaccgggac aggcacgagg cccggtcgac accggccgct cggcgtctct ttcaaaagga
     6541 accccagtcg aagccggcca tggcggaggt ggcgatggcg agctgctcga gcgacgcgtt
     6601 cagctggtcc agcgccagcg cccgctcggt ggcggtcggc gcttgcggcg tctcggcggc
     6661 gctgcccccc agctcctcga gcgtccagag caggctgctg agctcgaccc gcatgaccag
     6721 caggatctcc gccttttccg ggctgtccgg cgtgccgacc gtggcgcgca gcaaggcgag
     6781 gcgccggtcg agcccgcggc tggcccagcg cagcgaggcg accgtcgagc cgaaccggcc
     6841 gtcggcgatg cccgccacct cggctgccgg gctgaggctc gatgcatcac cgaagccgcc
     6901 ttcgtcgagg cggtcgcgca gccgggcgac atgcgccatg gcacccctta tgtcgagggc
     6961 cagccggtcc agcgtgccga agaccgccgg tgccgcgacc gccggcaggg ccaggaggag
     7021 gccgagcagc agcgtcgcgg ccaaagctgc cccggcccgc agccccgtcg tccggcatct
     7081 tgcttccctg cctcgtcgca cccgtctctc gccaccgtca tcgatccggg ccatactacc
     7141 ccgcccgagg tgaacagccg gtaaagacgc gcgtggcgcc gcaccggcgc ttgacggcgg
     7201 ccgccgtcgg cggtagcaag ccgcagtgcg cgacccgttt cggtcgcctt ttttgtgctt
     7261 cccttgaatg aggacgggtc atggccagac gctgcagtct gacgggcaag ggcgtgcagg
     7321 ccggcaacaa tgtcagccac gccaacaaca agacgcgccg gcgcttcctg ccgaacatcc
     7381 aggagcggcg cctcttcagc gaggccctcg gccgcagcgt gcggctgcgc atcgccacca
     7441 acacgctcag gaccatcgag aagcgcggcg ggctcgacgc cttcctgctc tccagcagcc
     7501 agaccgacct gccgtcggat ctcgggcgca tccgcaagct gatcgcggcc agcaacgccg
     7561 gctgagcgac tgcccggcca gccaccgggg acacggccca tgatcgccgc cgccggcctg
     7621 gcgatgacgg tgatcgtcgt cgcctcgaac atcctcgtcc agtttccggt caatgactgg
     7681 ctgacctggg gcgccttcac ctaccccgtg gcgttcctgg tcaccgatct cgccaatcgc
     7741 cggctcgggc ccgggccggc ccggcgcgtc gtctatctcg gcttcgcgct ggccgtgctc
     7801 tcctcgatcg tgctggcgac gccgcgcatc gccctcgcct ccggcacggc cttcctcatg
     7861 gcccagctcc tcgacgtgag ccttttccat cgcctccgtc gggcgacctg gtggcgggcg
     7921 cccctcgtct cctcggccgt cgcctcgacc ctcgataccg ccctcttctt caccctcgcc
     7981 ttcgccggca cgggcctgcc ctggatcggc tggggcatcg gcgatctcgg cgtcaagctc
     8041 gccgtggcgc tggccatgct cgtgccgttc cgggtcctga tgggccgcca ggacgcgctg
     8101 ccaaagcacc tgcccgccta gcaggctagg agccacgcca actgaagacc agcagcaaag
     8161 tgcgctgcag cggcgactgg ttgtcgtcgg cgaccagcag caggaggcgg ctgccatccc
     8221 cgaggtcgag gctggtgatg ccctcgtagt tctcggcgag cggcccggcg ccgatgcgca
     8281 ggagctcctc gccgaccatc gggccggcca tgggcccggc cgatgcccac tcctgccgcc
     8341 caatgaccgt cacccgggtc tgccagccgg tcaagacgcc gaccgagcgc tccagcacca
     8401 ggagccggtc gccggcgatg gcggccccga ccgggctgaa gccgcgtgcc gtcccatagg
     8461 tgaccggtgt cgatccgtcg ggcccgagcc gggagccccg ggcgatgtcg gccgcctgcc
     8521 gcccgccttc ggagagcacc aggaacgagc cgtcgccgag cccggtcgcc gcctccagcc
     8581 cgcgattggc cgggctgtcc ccggtcagcg atccgtcatg cagccgccgg gtggtccagc
     8641 cgccggcccc atcggcctcg agccgacgca ggctgccgtc ctgctcgaag ccgacgacga
     8701 ggccgccatc cgcgtcgagg gcgagggcct cgctgtcgag cgcggcccgg tcggccgtgt
     8761 cccggtggat ggaccccgcc cgccagccgt cgagccgcag gttgcggggc agctcctcga
     8821 gcccgagccc ggcctgccag agcaccgccc ggtcgctgag cagccacagg ctccggccat
     8881 cggtggcgat gcccgagaag ccgccgaagc gcgggtcgtc gccggtgagc tcgaaggcca
     8941 ggaggagctc gaccccgggt gccgtccgca cgcccgcggg ctcgacctcg aggcgagaga
     9001 tcgtgacaga cggcgtggca gaaggtatgg cgctctcggc gacacagccg acgagtgcga
     9061 cgagcaccca gccgagcgcc aggaggccgg cgagccggcg tcggcgcggt cgtgccgccg
     9121 gtcctttcgc ccacgcgtcg gcgcccgttt ccggcccgtc gaaccaggct gatcgatgtc
     9181 ccacgctctt gacctggccc tggtctacgc tctggtcgtg caatcggccc acctcctgct
     9241 cgccgccggc ggcggctggg cgaccctgcg cggcctcgtg ctcgagcggt cggcccaggg
     9301 ctggatcgcc ctcgcggccg ccatcggtat ggcactcgcg acgggcgccg tcaccggcgc
     9361 gctggcgccg gccgccctca cctggctgaa gctcgccggc ggcgctgccc tgctcgccac
     9421 cgtcgcctgg gtcgagctca cgccgcgtgg ccccttctgg cgggcaatgg ccggcaatgc
     9481 gccggggctc gtcagggccg ggatcgcccc gcgcccgggg ctgcccctgg ctctcggcct
     9541 ctgcctcgca gcgctcgccg gcgtcgcggc cccggcaccg ccgacggctg ccaactggct
     9601 gccgctcgcc tgcctcgccc tcctctggct cgccgggacc gatcatcgcc ccggcctgat
     9661 ggtccgtcgc gcggtcctgg cgttcctcgc cggtgcgctc gtcggcgggc tcgccttgct
     9721 gctgccgccc cagccgcatg ccctggttgt cggcttcctg accctggcgg tcgtcgggct
     9781 cgccctctgg cgaccgtcga cggccgtcgc gaattgagga tcgacatggg cgcgcaggcg
     9841 atcgccctcg ccccctatct ggtgctcccg gccgtgctgc tggcgctcgg cctgcctcag
     9901 gccaccgcct tcaccggctt caccgccggc ctcgtgctgc tcgtcctcgc cgcccacatc
     9961 gccgtcggcg tgctgcgcct gccgtttctc tgcctcggcg gcctggccgc cgcaggcgcc
    10021 gccttcgtcc aggcgctgag cggcctcgcc gggctcgagc cctgggcagc gctggccatg
    10081 gtcccggccc tcgggctcgc cctcggcggt gctttcggcc tcgtgctccg ctggctgccg
    10141 ggggacggcg aggagaccgg aaccgccctc ttgagcctcg ccctcctcct gccgctcgcc
    10201 gccctgccgc tcgccgccgg cggcacccgg ggcgacgggg tcgccgtcga ggacgccatc
    10261 gtgctcgtcc cgctcctgag cctcctcgcg gttctcgccc gcctcttcgc cggctcgcgc
    10321 ctggcggagc tcgccgcagc cgcggcggcc ggcgggcttc gagccgaggc tgctggcctg
    10381 gatctggccg gctggcggat caccgggctt tgcctcgcgg ccgccatcgc cacgaccgcc
    10441 ggtgccgtca tgctcatagg accggcgccg gccgcgtccg cgtcgatgga agcctgggcg
    10501 gcgctcgccg tcgccctctt cgccatcggc cggctcggcg gcgcgcgctt cggcggttgc
    10561 ctcctcgccg ccctgccgct ggccctcctg ccgcggctga ccgtgaccct ctcgccggcc
    10621 ttcggcgatc tcactctggc gatggcgctg gccgcgctcc tgctccaggc ggtggtccgg
    10681 cgcgacggct ccctcgcctt gctcgcccgg ccggcgaggc cgcgctccgc cgcacccttc
    10741 gccgcggcgc gccaggagcc ctgaccggga gatgagcgat ctcgccctcg accagccgct
    10801 gcaccctttg ctcgagctcg tcggcgtcgc cgtgcaccgg ccggggctgc caccgctcga
    10861 gcctttgacc tgcgccttcg acgccgcccg gaccaccctc gtggtcggca tcgagggctc
    10921 gggtcgggcc accctctgcg acctcctcgc cggccggctc ctgccggacc ggggcgtgat
    10981 gcgcctcgac ggccagcgca tcgaccggct gcccgctgct gcccgggtcc ggctcggcat
    11041 cgtcacctgc gggccggagc ggctggtgca gcacggcagc gtgcgggcga tgatgctcac
    11101 cgcccggctc gcggtgaccc ggccggtctg gtcgctcgcc ttcggcctcg gccggccgag
    11161 ccgccaggac cgcgaggata tcgaggagct gctcggcttc gtcgggctcg accacctggc
    11221 ggcgcgtggc gtgccgacgc tcggcggcct cgaggcccgc ctcgccgacc tcgcccgctg
    11281 cctcgccatg cggccgcgcg tcctcgtcgt cgggcacggg ctcgccgggc tcgaggcacc
    11341 cgaccgcaag gccttcgccg cgctcctgca gcgcgtggcg gcggccggcc ccgcgatcgt
    11401 cgccatcgac gacgacctcg ccacgctcgg cgcgtatgcc gacctttgcc tggtgctgca
    11461 tcgcgggcgg ctcctcgcca acgcgacgcc acgggcgatc ggcgaggatt cgcgggtgtt
    11521 cgaggccctg acgggggccg agctgtgagc gccgccctgc tccgcctcga ggcgctcgac
    11581 atcgtcgccg gcggtgcctg gctgctcgaa ggcatctcgc tcgaggtggt cgagggcagc
    11641 accgtggccc tggtcggcgg gcccggcgcc ggcaagtcgc tcatcgtcga tctggtcctc
    11701 ggcctcgcca gcccgcgggc cgggaccgtg cgcctcgacg gccggaacat caccgccctg
    11761 ccgccgctcg cccggcagcg atccggcatc cgcgccgcct tcgccgagcc gccggtcttt
    11821 cccggcctca ccatccgcga gcatctggcg ctggcggtgg ccggcggccg gctccgggcc
    11881 agggcgctga gccgtctcgc cgagtacctg ccggagctcg agggccgcct cgatcagccg
    11941 gtcgggtcgc agggtgccgc cgtcctcagg ctggtcgacc tcgcccgggc gatgctcggc
    12001 atgcctcgca tcctgctgat cgacgatctc ttccccgccc tgggcgccga gcgggccggc
    12061 gagctggtcc gctccctcgc ccgcgacggc tacaccctgc tgctcgccga ccgctacggc
    12121 gaggcggtgc tcgatcatgc ggatcacggc tacgtgctgg cgcaggggac gatcgtcgcc
    12181 gagggtgccc cggcgacgct tcgggccgat ccccgcctgc tcgccacctg cgccggcgac
    12241 tgcagcgcct gggccgagat acctgcctga gccaggctcc cggcccccga acgcaaaacg
    12301 gcccgccgag gcgggccgtt cgctaccgat ggatgtacgc cgggctcagt tgcggttgcc
    12361 gaagagctgc agcagcatca tgaagaggtt gatgaagttc aggtacaggc tgagcgcacc
    12421 catgatggca cccttggtgg cgatctcggc gctgttgaac gagtagtact cctccttgat
    12481 ccgctgcgag tcgtaggcgg tgaggccggt gaacacgagc acgccgatca cgctgatcgc
    12541 gaactgcagc gccgtgctgc cgatgaagat gttgaccagc atggcgatga cgatgccgat
    12601 caggcccatg aagaggaacg agccgtattt cgagagatcg cgcttggtcg tgtagccgta
    12661 gaggctcatc gccccgaagg tcgcggccgt gatgaagaac acacgcgcga tgctggcccc
    12721 ggtgaacacg aggaagatgc tggccagcga caggcccatc accaccgcga acgaccagaa
    12781 ggtgatctgc gcggcgctga cgctcatggt gcggatcttg gcaccgagga agaagatgaa
    12841 gcccaagggc gccagcatca ccacccactt gagcggcgtg ccgaagatca cctgcaggag
    12901 ggccggcgac gacgccgtga cgaaggccgt gaggcccgtc aggaccagcg cgagccccat
    12961 gtagttgtag accgagagca tgtggctgcg caggccggcg tcgatcgacg tgtcgacgac
    13021 cgcaccgtgc gccaaccgct gatccatgcg aggttccata ttttctgacc cttttccagc
    13081 gagcggatgt gccccgttac gtccctgcca atatggccaa cggccgccgg cgatcaagcg
    13141 gcaccggcag tcgcccgatg ccgccacggt cgcttcggac cgaacattgc ggaagtttca
    13201 tgcgtttttc ggcattcggc aaggctctgg ctaccgttcg cgcaggatga gggcggccgg
    13261 cacggcgaga atgtggcgga gcccgagggc gccgagcagc agcgtggcga gcaccgccac
    13321 caccggcacg acggtcaccg gcaaggccga ggggtgccac ggaagcccga aggcgaagcg
    13381 cagcacggcg aagctcgcga ccagcccgag caaggcgccg agcagcgatg cgacgacgcc
    13441 gatcgccgcg tactggccga gaaagatccc gccgatctgc cggcgctccg cgcccaccac
    13501 cttgagcagc acggcctggc gaagctgctc gcgccgggcg gcgacgagcg cgctggtcag
    13561 caccgcgagg ccggtgacca gggtgaccgc cgccatcacg cgcacggcga ggccgatctt
    13621 gtcgaggatg tcggcgacct tcgcgatcag ctcgtcgatg gcgaagggcg tcacgttcgg
    13681 cagctcgcgg gccatccggt cgagcagggc cgcctgctcg cctttcggca cgtcgaccgt
    13741 ggcgacgagc gtgtgcggcg cctggtcgat gacgcccggg ctcagcacga agacgaagtc
    13801 gatccgcccc tccgaccagt cgatctcggg gcggagattg gcgacctcgg cctcgatcag
    13861 ccggccgagc acgttgaagg tgaccgtgtc gccgacgccg atgccgtagc ccagggcgat
    13921 gtcgtcctcc atcgagacca gaggcgggcc ggcatagtcg gccggccacc actcgccggc
    13981 gaccagcgtc gtgtgctcgg gcggggccgc gcgataggag agcccgcggt cgcggcgcac
    14041 cgtccagcgg gcgccctcgg cgatcaccgc ctcgtcgacc ggcacgcccg cgatcttcac
    14101 cacgcgggcg cgcagcacgg gctccatctg caggacgctg gcatccggaa acgcgccgac
    14161 caccgcctcg aactccgccc gctgcgtgcc ctggatgtcg atgaagatgg tgctcggcgc
    14221 gttcatcggc cggttctcga ggagctcgcg cgagagcccg ttctcgacca gcagcaccgt
    14281 cgtcaacacc gccgtgccgg ccgccaatgc gaccaccgag gccaccgcca tccgctccgc
    14341 ccggcggagc tggccgagcg ccaggcgcca catcccggtc gtgtgccgcg ccagccaccc
    14401 cgcaccctcg agcccgagcc gggcgagcac ggccatggcc agggccgcca ccagcacgat
    14461 gccgaggaag atcacggcca catggggtgc gggcaccgcg aggagcgccg tcagcaccag
    14521 cacgaggccg agcccgagca gccacggcca ggcttggcgg cgcaggggcc gagggccggc
    14581 agccagcgca tcgtcgcgga agagggcggc cggcgtcacc gagaccgcct tcaaaagcgg
    14641 cagcacggtg aagagggccg ccgtcgccga ggcgaagagg gcggccgcca ggatcgcccc
    14701 cggctggacg ccgatcgcca ggtcgatcgg gatcagcccg gccggcagca ggtgcacgcc
    14761 gaacggcagc gccgtgccga gcgccgcccc gagcacgagc ccgccgcagc agagcagcag
    14821 cacctgcagg ccgcagacga gaaagacgtc ccgggtcgtc atgccgacgc agcgaaaggt
    14881 ggcgatgctg cgccgacggc tctgcagatg ggcgtcgacg acgatgccga ccccgagccc
    14941 gccgacgagg agggcggcga tcccggcgag cgagagatag cccgccagcc ggtcggcgtt
    15001 gcgggcgatg cgcggctgca cgtcgttgaa ggtctgcgcc cgccacgctg cctcggggaa
    15061 gtcgcggcgc aaggtggcga cgtaggcccc ggcctcgctc cggtcggcca gcgccaggcc
    15121 gtatttgtgg cggaccagcg cacccggggc caggatgtcg agggcgtcga gcgtcgcccg
    15181 gtcgatcatc acccttggac cgagcccgac gaagccgccg atctggtccg gctcggcggt
    15241 cagcacgccg cgcaccgtga cccggccatt gccgagggcg aggctgtcgc cgacggcgat
    15301 agcgagccgc tgcagcagcg ccgcctcgac caaggcgcct ccgtcggcca gcgcgtcctc
    15361 cagcgtcatc agcggctcga tgcccaccac gccatagagc ggataggccc cgtcgatcga
    15421 cttcagcgcc accggcacct gccggccgtt gtcggcaaaa gcgatggcgt tggtggtgac
    15481 cgtatgcgcg accgtggcac ccgccgggac gatcgcccgg agctcctcgg gcggcagctc
    15541 gcgataggtc gattcgatca ccagatcgcc gccgagcagg gcccgcgcat cgcgctcgag
    15601 cgtgtcggtg aggctggcgc ggagcgagcc gatgccggtg atcgtcgcca cgcccagggt
    15661 gaggcagccg aggatgatga gggccgccgt gctgctgccg cgcaggtcgc gccaggcgat
    15721 gcgccagagg gcgccgaggt tccaggcgcc gcccgcgcca gccctcaaga cgacgccctc
    15781 gcgaacgcat cggcggcgac gtcgcccgcg tccagccggc cgtcccgcat cgcccgggtc
    15841 tcgtcgcaga gcgaggccag ggtggggtcg tgggtgatga ggaagagcgt ggcgccgtgc
    15901 cgctcgcgcg cggcgaagat cagctcggcg atctgccgcc cggtgctccg gtcgagattg
    15961 ccggtcggct cgtcggcgag cagcagctcc ggctcgttcg ccagggcccg cgccagggcg
    16021 acgcgctgct gctccccgcc cgagagctgg cccgggtaat gatcgagccg ctgcccgagc
    16081 cccatctcgg cgagcccggc ccgcgcccgc tcgaagccgt ccggctcgcc ggccagctcg
    16141 agcggcaggg ccacgttctc ctgcgccgtc atggtcggga tcaggtggaa ggactggaag
    16201 agcacgccga cgcgcttcct gcggaacagc gccagcccgt cctcgtccag cgtgccgaga
    16261 tccgtgcccg ccacctcgac cctgccccgc gtcggccgct cgatgccgcc gatgatcgcg
    16321 agcaggctgg acttgcccga gccggacgga ccgacgacac tgaggctcgt gccggcagcc
    16381 acgtccaggt cgatcccgtt caggatgtcg accgggccgg cgctgctccg cagggtcaga
    16441 tggacgtcac gcaagcaaat catgagcacc ggaccttcta ccaattatcg agctgccacc
    16501 tatcgggcga tcgacggaca acgtcccctg caacttggga cccggcgccg catcctcaag
    16561 cgggctgccg cgctttgcct catcgccgcc tcgcccctgg ccacccgcag ggccggcgcc
    16621 gcgggcccgg aaccctgccg catcgccatc ctcggcgact cgctcaccgc cggctacggc
    16681 gtcgccctcg aggatgcctt cccggtgcgc ctcgaggcgg ccctccggga agcgggccac
    16741 gcctgcgaga tcatcgacgc cggcgtctcg ggcgacacct cggccggcgg ccgcgcccgg
    16801 ctcgactggg tgctcggcga cgaccccacc cacctcctgg tcgagctcgg cggcaacgac
    16861 gcgctgcgcg ctttgcccgt cgacgccatg gaagccaacc tcgccgccat catcgagggt
    16921 gcgcaggcca ggggcgtctc cgtcatgctg ctcggcatgc tggccccgcc caacctggga
    16981 tcccgctaca ccgaggcctt cgccgccgtc ttccccaggc tcgccgaacg ctacgccgtg
    17041 ccgctctatc ccttcttcct cgacggcgtc gccgccgacc cggcctacct gaccgaggac
    17101 ggcattcacc ccaacgccgc cggcgtcgcc cggatcgtcg agcgactcct accttcggtc
    17161 gaaatctggc tcgacgagtc tgccgattga gcctctgtca agcgctcttt gcgcttgacg
    17221 aaagcccgct gctggcctcg attccccgcc cgcgggaccc gcaccgcagc cgtccggaat
    17281 cagtgactta cccacatctt gttgataatg gtggggatag tcgtggtcat gtgcgtcagc
    17341 ttgtggataa gtggcgggac agacgctgaa cgaaccgtgc gcttccgaga tgcgccccgt
    17401 tgcggcaaaa cgtccccgtg gcatagcggg gaatcgcccg ccgcggccgc ctggcgcacc
    17461 tgtggggcaa cttgtcgggg cgctgcacgg agctcgggcc attgcccggc aagcggcccg
    17521 cagaggtcag ctcgagaagg gagaacggca tgtcggactt gtacgaggtc agcgcggaat
    17581 gggcggcatc ggcgcattgc gacgcaccca cctatcgccg gatgtacgag gaatcggtca
    17641 acgatccgga gaccttctgg ggccgtgaag ccggccggct gagctgggcc aagccgccga
    17701 ccaggatcaa gcgcacgacc tacagtggcg acgtctcgat tcggtggttc gaggatggcg
    17761 agctcaacgt ctgctacaac tgcgtcgacc ggcacctccc cgcccgcgcc aaccaggtgg
    17821 cgatcatctg ggaaggcgac aatcccgacg tcgacctcaa gatcacctat cgcgacctgc
    17881 atcgcaatgt ctgcaaggtc gccaacatcc tgaaggacat gggcgtcaaa aagggcgacc
    17941 gggtcaccat ctacctgccg atgatccccg aggccgccta caccatgctg gcctgtgcac
    18001 gcctcggcgc catccactcc gtcgtcttcg gcggcttctc gcccgacagc ctcgccgacc
    18061 gcatcaacga ctgcggctcg aagctcgtcg tgaccgccga tggcgggctc cgcggcggcc
    18121 gcagcgtgcc cttgaagggc aatgccgaca aggcgctcgc caagtgcgcc gatgacgtca
    18181 agctcctcat gttccgccac accggcatcg acgtgccgat gaaggcaggc cgcgacgtcg
    18241 acggcatgct ggccatgaag gaagccagcg acgaatgcgg ctacaccgtg atgaacgccg
    18301 aggaccccct cttcatcctc tacacctccg gctccaccgg caagccgaag ggcgtgctcc
    18361 acacctccgg cggctacctc ctcttcgccg ccatgaccca ccagtacgtc ttcgactacc
    18421 aggaaggcga cgtctactgg tgcaccgccg atgtcggctg ggtcaccggc cacagctaca
    18481 tcctctatgg cccgctcgcc aacggcgcca ccaccctgat gttcgagggc gtgcccaact
    18541 accccgacat gtcccgcttc tggaacgtcg tcgacaagca cgccgtctcc atcttctaca
    18601 cggcccccac cgccatccgt gccttgatgc agaagggcga cgacccggtg aaggcgacct
    18661 cccgcaagag cctcaggctc ctgggctcgg tcggcgagcc catcaacccc gaggcctggc
    18721 gctggtacca caccgtcgtc ggcgaaggcc gctgcccgat cgtcgacacc tggtggcaga
    18781 ccgagaccgg cggcatcatg atcaccccgc tccccggcgc catcgcccag aagcccggct
    18841 ccgccaccct gcccttcttc ggcgtccagc ccgccctcgt cgacaacgac ggcaagccga
    18901 tcgaaggtgc cggcgagggc aacctcgtca tcaaggacag ctggcccggc cagatgcgca
    18961 ccgtctacgg cgaccacgag cgcttcatcc agacctactt caccaccttc aagggcgtct
    19021 acgtcaccgg cgacggcgcg cgccgcgacg aggacggcta ctactggatc accggccgcg
    19081 tcgacgacgt catcaacgtc tccggccacc gcatgggcac cgccgaggtc gagagctccc
    19141 tcgtcgccca ccccgccgtc gccgaagccg ccgtcgtcgg cttcccccac gacatcaagg
    19201 gccagggcat ctattgctac gtcaccctgc agaacggcat cgagccctcc gaagccctgg
    19261 aaaaggacct cgtcaggcac gtccgcaacg acatcggccc catcgcctcg cccgaccaca
    19321 tccagtgggc ccccggcctg cccaagacca gaagcggcaa gatcatgcgc cgaatcctcc
    19381 gcaagatcgc cgccaacgag cacgacgccc tcggcgacac cagcaccctc gccgaccccg
    19441 ccgtggtcga cgacctcgtc gccaaccgca agggggcgtg acgagaaggg aagacatgcg
    19501 gggagtatga tactccccgc accccaccgg tttatcctgt ggaggaagag atgaaagaac
    19561 ggctggaata tgccggcttg gcttttcaaa ccacgtcgtt atgtgtagga atattatacg
    19621 tcacaggatt ctatataaat tctcttttta ctagcaattt tggcatcttt cgtactgaat
    19681 tgttaagact ggattacatc aaagtcggat ttgtatttaa tcttattact cttggcattg
    19741 tctttgtgcc aattggaatt gtacttttgt ctttttctgt gcgatcttcg tcgaatctgc
    19801 cgaactttta tcttggcatt atcggaaatt cactgaacac catcctgttt tttggtgtac
    19861 cgatattttt tgcgttcttc gcaactagcg ttgaatggaa tttggctttc gtagagcctt
    19921 tgtatatctt tactactgtt gaatcagctg taataacatt cttggtaatt acattcttcg
    19981 ggatggcggt aattccagcc ttagaaagat tactctatgg aacaaatttt ttcgccgctt
    20041 tgttgtaatt cattgcgttt tagttgaatc tcttagattt ttgtcctttg gccttgctct
    20101 cgctattgct atcaagattt ttagcgccat tgaatggata ccggcgttgt tacgcggtgg
    20161 tttatactat ctattgactg cgattgtctt cgtcttaggt atggttgctg ccggattctg
    20221 gataaggaag attcgacacg tcaaatttgc cggactggtt tatgtactgg tagcattcgg
    20281 gctagcaatt ctattttacc tctcggtcac atcctatgtt ttcggtgtat acaagttcat
    20341 tccaaagaat cgtggcggcg ggctgccggt aacagagatt tatttcgatt catctaatca
    20401 taaaatgttg ctttcagagc tagctagctt ttcaccgatt attggctttg gtccatttta
    20461 tattgttgaa gagaattctg actttttgtt cctggcttct gctgagatgg aaaaatggac
    20521 gatcgattat gttcctattt ttgtcatgcg caagagccat ttggatcata tcttcattcg
    20581 aaggatacct agcggttttc cacgaacaat tagctctgcc agtagcagtc ggccttagac
    20641 atcaccctgt accttcaggc cgtcctcgaa gagaatgacc cgacccaatt ctgccacgcg
    20701 ctgggccaag tcgcccacga cattggcctc gcactcaaac cctctgccga gcaaaaataa
    20761 agggttcgcc cctaagatgc actctattta gatgaagact gaacgactac cggctgacgc
    20821 cggtaggttc gatgtgatct gcgagatact gaagtatcgt gtactgggtg atgttgccgg
    20881 cggtggtgga gaaataaccg cgtgcccaga aatggcagcc ccagtagcgc ttgcgaatgc
    20941 tcgggaattc ctgctggatc ttgcgcgatg agcgtccttt gatgcgccgc atcacgtcgc
    21001 tgacgctgtg ccgcggcgga atggccagga acaggtcgtg tccctggacg tggtgtagct
    21061 ggcggtggcc atcggcggtt cttcgtggag ccgaaaaggg gtctggactc tttttagctt
    21121 gggactttga gggatagctt ttttttacct ctgcgggcac ggcagcccag cgcacgtccg
    21181 gggacacaac ccgcaaaccc aaatccctga acttcggccc cgtgcagacg ccggcgcagg
    21241 atcgtcgcaa taccgacgcc accactgccg ggcgaccacc aacaaggacc ggtggggcca
    21301 ggggaggctg tatagaccgg agacaaggtt tacatgcgtt cgaggacatg ggtga
//
LOCUS       DAOTFI010000057        21222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2979_length_21222_cov_6.953135, whole genome shotgun sequence.
ACCESSION   DAOTFI010000057 DAOTFI010000000
VERSION     DAOTFI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21222
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2979_length_21222_cov_6.953135"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="P5558_07655"
     CDS             complement(<1..187)
                     /locus_tag="P5558_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24221.1"
                     /translation="MAAGGKAGENLLDIVDVYKTFGGVRAVQGCTFGLVRGSITGLIG
                     PNGAGKTTLFNMIAGAFP"
     gene            complement(282..509)
                     /locus_tag="P5558_07660"
     CDS             complement(282..509)
                     /locus_tag="P5558_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24222.1"
                     /translation="MRPAPDEILLEITRLGSVQRVSAIDPSSLVEVVFQAPLTADHRA
                     IELLARQKLAYRLSRGAAPAPTPRHGGGTIV"
     gene            728..1855
                     /locus_tag="P5558_07665"
     CDS             728..1855
                     /locus_tag="P5558_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY24223.1"
                     /translation="MLGLAGGTASAQQGQQGQQAAMVQVDAVITEPLVQTVPVLGRIV
                     TRQEGPVASRVEGPVAEIGVAVGDRLATGDLIAVLDRKRLQLDSALAAAELAATEADL
                     AAARREVELLEQERDRLVALQGSAAFSRARLDDKIKEVHVAQSRIEAAAARLEQARVT
                     LDYRNADLADAEIRAPYPSVVVQKHVSAGAHVRVGDPVVTLVDDSALEVEADVPAERL
                     FELEPGTPIAFSIAGAGHAGGRHDAVLRAIVPMENPLTRTRAVRFTSVNGGLEAARAI
                     GQSVTVDIPVGAPRDVVSVHKDAVTINLGTRQVYVVGDDDVVQPRAVVLGAAIGNRFE
                     VLEGLAPGDLVVTRGNERLRPGQTVAYGSPANGDAGKDQKG"
     gene            1861..>4711
                     /locus_tag="P5558_07670"
     CDS             1861..>4711
                     /locus_tag="P5558_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY24224.1"
                     /translation="MNLIRLAIDRPIAVIAAVLMAVMFGYVALTTIPIQLAPDVVKPV
                     ISITTSWPGAAPAEVEREIVNEQEEVFKGLEGLERIESKSEDGQATITLEFSLDTDMD
                     RALLLVANRLQRVPSYPAEADEPSISTSSDDDSAIAWFVMTRVEGNDRPIESFGPYAE
                     DTFQDRLQRVAGVARVDVFGGAERELQVIVRPENLARYGLTVPDVVDTLRSAGISLSA
                     GDVAEGKRRYVVRTEGELTTPEAVETVVLRSIADPLTGRLARITVADIAEVRFGYSET
                     VATIRVLGEEALVGRAIRETGSNVIETMKGIHAAVEELNAGAMPNAGLQLRQVYDETV
                     YINSSIDLVTQNIYVGGTLAALVLLLFLRSFGATLIVSVAIPVSVIASFVAMAAMGRS
                     INVVSLAGIAFAVGMVVDAAIVVLENIYRLRQEGKSPAEAAYQGAAQVWGAILVSALT
                     TVMVFIPILTMKLEVGQLFRDIAVAISVAVLLSLLVAITLIPAMSKRLLGGADGSLVM
                     RRIPVVDDLAGLFVRAVLWTTRRVCASVPLSLAVVASVTLVTGYLAWANLPKLEYLPQ
                     GNQNLVFGIVLPPPGYNLETMTDIATDIEGALRGLWASETGPEPTADGIPKLEEFFFV
                     AFRGNMFVGGSSADMERAGALIGPLTAPVFREPGTFGFFSQPSIFGGGIGGGRTIDFD
                     ISGPDLETVLAIAQRATGMIAGLLPREQGHQFRPDPGLELGAPEVRLIPDRLRLADAG
                     VSARNFALSVDAFNDGLRVDEISVDGDQIDLTLKGPEGNAERTQGIDALPIVTAEGHI
                     LPAGSLARIEVTAGPTEIRHVERQRTVTLKVSPAPSLPLEVALDILQNEVVAPLEQQG
                     LPPGVQLRLSGTADQLTQTFDAMVLDLALAVVIVYLVMAVLFESFIYPLVILFSVPLA
                     AAGGVAGLFVLNLYTFQPLDMLTLLGFVILVGIVVN"
     assembly_gap    4712..4811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4812..5204
                     /locus_tag="P5558_07675"
     CDS             <4812..5204
                     /locus_tag="P5558_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY24225.1"
                     /translation="LDMLTLLGFVILVGIVVNNAILLVHQTLFELREHGRAPLDAIAE
                     AVRNRLRPIFMSTMTSVMGMLPLVLFPGAGSELYRGLGSVVVGGLSLSAVLTLLLIPP
                     LMALVLVRQQRRAATPAMVKGAAAAPAE"
     gene            5338..6861
                     /locus_tag="P5558_07680"
     CDS             5338..6861
                     /locus_tag="P5558_07680"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY24226.1"
                     /translation="MVAAAQPAPAPVPSPIDIAAPVARPEGDDRTRLAAASGEAGTAA
                     AANPLPLAVAEAMVQEEAAAEATPRGADAPPEAEAAPEAAAAGIETTSPAEAVEPGAG
                     PANGGTRPPAGGGAGFTTYDQGDIGDGLDPTGALGPTALDHGLVTNVPFSGHYGNDAS
                     GRGAGEPGGDSGGDGGGGPGDPQANRAPVACDDSFTAGENLVRKIALGDLLANDHDPD
                     GDPLSLVSVQGAENGTVRLGADGFLYFTPTHDYSGEAGFSYTISDGKGGLSTAEVRLD
                     VRANTDPHATDDNVNTTMNTLKVIEPSNLVNDDFDADGDALRLVSVQNAQHGEVWIGQ
                     GGKLYFQPDQGFVGEAGFSYTITDDRGGIDTARVDVHVHEKGWQKGEPGNHAPVATDD
                     NLTVRMDTLKVVQQSNLTGDDFDADGDALRIVSVQRAENGTVWLGQDGELYFRPDEGF
                     VGEAGFSYTIADDRGGLDTARVTVQVAAPEGGMPGPEGCGCGGEQVANLVPPAEHVV"
     gene            6961..7956
                     /locus_tag="P5558_07685"
     CDS             6961..7956
                     /locus_tag="P5558_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HRY24227.1"
                     /translation="MIETPYLLFLGDVPDQLAAKTAQGVVHWRPEACAGQLRLPGCKA
                     DLGLPELSIDAGAKAGVRTFLVGVANAGGVLPPHWVANIVEAIEAGMDVASGLHVRLG
                     DVPEIADAARRKGARLHDVRHPTRTFATGKGTKRAGKRILAVGTDCSCGKMYTTLALE
                     REMRERGMQADFRATGQTGILITGEGVSIDAVVADFISGAVEWLCPANDPDHWDLVEG
                     QGSLFHPSFAGVSLGLLHGAQPDLLVLCHEPTRTHMRGLPHQKLPGLKECLERNLEAA
                     RLTNPDVQALGVSINTAALNEAAARDYMAEVEQQLGLPTVDAFRTGVARLVDALS"
     gene            7953..8936
                     /locus_tag="P5558_07690"
     CDS             7953..8936
                     /locus_tag="P5558_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRY24228.1"
                     /translation="MTKRRLDIAVERFPLVKPFTISRSSVTELELVTATITEGEHAGR
                     GECRPYPRYGETTDGVVEDIRARAGLIEAGEGCAALLEAMPAGAARNALDCALWDLEA
                     KLAGRPVHDLLGLPAPAPVLVTYTIGLDSPAAMAAEAKAVSRPLLKLKLGGSDDLERV
                     RAVRAAVPETRLIVDANEAWSQEMVVAWMPELAAMGVELIEQPLHAERDRALEGLARP
                     VPLCADESGHGIEDLDALAPLYDFINIKLDKAGGLTAALALADAAEARGFRLMLGCMM
                     ATSLAMAPAFVLAGRARFVDLDAPLLLARDREHAITYDGAMMQPPPQALWG"
     gene            complement(8958..9644)
                     /locus_tag="P5558_07695"
     CDS             complement(8958..9644)
                     /locus_tag="P5558_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HRY24229.1"
                     /translation="MKRSRVNAIIVEADAMIRHHGFVLPPFANWTPDELKARRETARH
                     VIDARCGWDITDFGTGRFDEVGLAVFTLRNGRLADLQRGRGMCYAEKLLVLRQGQRCP
                     SHTHFIKAEDIINRGGATLVVELFGSDEQGGFAADRGGTVHRDGIRHDYAPGERLELA
                     PGQSVTLAPGDWHALWTKGGDVLIGEVSTVNDDETDNIFREPVGRFAEIEEDEPPARL
                     LVSDYRTWLA"
     gene            complement(9641..10942)
                     /locus_tag="P5558_07700"
     CDS             complement(9641..10942)
                     /locus_tag="P5558_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24230.1"
                     /translation="MDPADRATLVENAQAILRENDRGGYTVPTRGLYPFQWNWDSCLT
                     ALGLGHQDEDRAWREIETLLAHQWPDGMVPHIVFHVPDEGYFPGPEIWRTNRPVPTSG
                     ITQPAVAGFAVRRLFERARDPAMAAERARPLLAAIDRWHQWFFAHRDPAGEGLVAIIH
                     PWESGRDNSIDWDEALARVPTDGIEPYTRRDLQHADAAHRPTNAEYDRFVWLLERFRG
                     LGWDNARLHDDSPFQVVDPGFNAILIRSCRDLAVLAERLGDAKLAARNHAFAEHGLVA
                     MAGLWSVTDGQYLARDRTTGRLVGSRSVGGLLAAFAPIPQDRARALGHRIETLAGRSR
                     HLVPSHDPADPRFDAKRYWRGPVWLIVNYMIADGLAAAGEAAIAARIRADSIDLITRS
                     GFAEYYDPLTGEPCGGDRFTWTAAMVLEFLETSGEQAGEPG"
     gene            complement(10956..11888)
                     /locus_tag="P5558_07705"
     CDS             complement(10956..11888)
                     /locus_tag="P5558_07705"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY24231.1"
                     /translation="MPAKGIYAAVVTPVEPGGAISVERYCRHARWLLRQGCTGLGVFG
                     TTSETQAFSVAERQAGLEAYVAAGLPVERMILGIGCCARSDTLALARHALGLGVTNLL
                     MLPPFFYKNNSDEGLYRAFAEVIDALAEPRLALLLYHFPQMSGVPVTHPVIERLLKRY
                     PDTLEGIKDSSGDWEHTASLISNFPELTIYSGADGHLVRNLEAGGAGTFSAAANLAAG
                     PSARVFKAFNDGDRSAAEAGMKLVSDVRNLLTSYPLVPGIKHVIADGQHDEVWRIVRP
                     PMVELDGAKGEALVAALDEAGYVYDPELYSVAGA"
     gene            complement(11977..12537)
                     /locus_tag="P5558_07710"
     CDS             complement(11977..12537)
                     /locus_tag="P5558_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRY24232.1"
                     /translation="MRFEVDAGSENPCPKWCVALFSPFARRLLAALLLTLALAAGAGP
                     AAAQSTGLPLPRFVSLAASETNVRTGPAETFPIRWVFTQRGIPVQIVEESFDWRRIVD
                     HEGETGWIHASLLSSRRTVLVTGDVRPLRRTPRSDARVVLLAEPMVIGDLLSCEGAWC
                     LVEIDGRRGWLERGQFYGVAETETVD"
     gene            12725..13711
                     /locus_tag="P5558_07715"
     CDS             12725..13711
                     /locus_tag="P5558_07715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRY24233.1"
                     /translation="MPRKRPLVVVTRKLPDVVETRMMELFDARLNDSDTAMTRAELVE
                     AVKACEVLVPTVTDRIDAALLAHAGEQLKLIANYGTGVDNIDVEAAYKRGITVTNTPG
                     VLTEDTADMTMALILAVSRRLVEGERLARSGEWGGWSPTSMLGHRIHGKRIGILGMGR
                     IGSAVARRAQGFGLAVHYHNRRRVHESVESELEATYWESLDQMLARVDIVSVNCPHTP
                     ATFHLLNARRLKLLRREAIVVNTSRGEVIDEAALTRMLRDGDIAGAGLDVFEHEPAIN
                     PRLRQLENVVLLPHMGSATLEGRRDMGEKVIINIKTFADGHRPPDRVLPSML"
     gene            13822..15117
                     /gene="sbmA"
                     /locus_tag="P5558_07720"
     CDS             13822..15117
                     /gene="sbmA"
                     /locus_tag="P5558_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HRY24234.1"
                     /translation="MFTSFFPQPKLFFLSVVLWSAVATALWYGGADGWGALIGLGTTP
                     EGTETVLGLGYFVTPEFLWFYIYYAIVVALFAAFWFRFDPHPWQRWSILGSALILFIT
                     YFGVQISVAVNNWRRPFFDDVQQALGENSTVTQADLFALLGIFAQIAFLSIFIFVLSR
                     FFVSHWIFRWRTAMNDYYMARWQRLRHIEGASQRVQEDTMRFAGIMEGLGVSAIDAVM
                     TLIAFLPVLWELSKYVEELPLVGAIPAPLFVAALVWSLFGTGLLALVGIRLPGLEFRN
                     QRVEAAYRKELVHGEDQADRAQPMTVHELFGYVRQNYFRLYFHYMYFNVFRSLYLQAD
                     NIFVYILLIPTIVAGAAVGFTFGVLQQILTAFNQVSNSFQYLVNSWTTIVELLSIHKR
                     LMAFEAAIEDKELPDIDKEWLEIHGAGDDGGVRTVGSGI"
     gene            complement(15218..16993)
                     /locus_tag="P5558_07725"
     CDS             complement(15218..16993)
                     /locus_tag="P5558_07725"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRY24235.1"
                     /translation="MPPQPDLSARTGSAGGPPPEIGFRDAFRQLRVLFGALRRSPYRR
                     ACFLLTFGLVAVICLNMLGQIRLNAWQGDFFDALEQRLPGALLDQTLIFFAIVLVLLL
                     FVVAETWFRELLEVRLREWLTRDLLDQWVTGRRPWLLGFQGDIARNPDQRLQADARQL
                     TELTITLGIGLLRSTLLLAGFLGVLWTLSAGAVFRLGESELHIPGYLVWCALAYAVSG
                     SWLTWRVGRPMIGLNAERYAREAELRFALVRISENAEAIALHDGAAGERRLLDQPVDA
                     VVAITRRLAYALARLTWITSGYGWLGLIVPVLAAAPGYFQGQLSFGGLMMTVGAFNQV
                     QAALRWFVDYFPAIADWRATLFRVVALREILPGLDTLAEGEGRITYVEHTEPRITLAD
                     LRVALPGSEVVLAEPLVELAPGERLLLTGPKGVGKSAVFHALAGLWPWGSGTVHLPPR
                     DRLAFLPGRPYLPLGSLRAVLAYPALPDSPASTDTDPGAALERTGLARLVPLLDTERR
                     WDQELGTDEQQRLVAARLLLHRPAAVVMDEPMSALDPDRRELLADLFAHELEATTVLA
                     LGPPVQDPFYHRRITIERRPAGSGT"
     gene            17164..17967
                     /locus_tag="P5558_07730"
     CDS             17164..17967
                     /locus_tag="P5558_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRY24236.1"
                     /translation="MTEERYEVFAVRYGTVAARTRRENFMGLDPHETAPMPLDYYVWV
                     VRNEARTIVVDTGFDHAEAAHRGRRLERLPREGLALVGVDTLTVEDVIVTHLHHDHAG
                     TLGDFPAARFHLQEAEMAYVTGACMCYPALRMPFSVEHVCEMVRNVYSGRVVFHDGDA
                     EVAPGVTVHRLGGHSKGIQCVRVQTERGPVVLASDTAHFYENVEQGKPFIITHDVEAT
                     LRGYDRLRKLAASPAHIVPGHDPLVLQRYPAPDERLAGIVCRLDVMPQG"
     gene            17969..20548
                     /gene="mprF"
                     /locus_tag="P5558_07735"
     CDS             17969..20548
                     /gene="mprF"
                     /locus_tag="P5558_07735"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HRY24237.1"
                     /translation="MATAAGEAGAKPGSRWHLPHLAPLVGVAVVALAAYVIHRVSAEV
                     HLADIEAALAATPLRSILLGLLLTATSFLAMSLYDVLGAEQVAPGRVPKAIAAFAGAA
                     SFAVANAVGFHVLTGVPIRYRLYAGAGLDMAQVGHIVGNALVAFWLGVATLLGAAMLI
                     DPVGVPLLDRLDPRLDRVLGAVILAVIAGMVLWLGRNPRTLALFGWRLVLPGAAGMLA
                     RIGLGVVDVGCAAAALYVLLPADVQPGYAVFLLVFLTAIVAGAASHVPGGLGVLEATV
                     LVGLGAGTNPDVIAALVVFRVLYYLLPLALAGLTLALFEGRRGLAPVAGKALRVGRPL
                     VPPLAAGLVFMGGIVLLLTGNLPAEGSRLDRLGHLLPLPFAEASHMASSLVGLLLIVL
                     ARGLLARMALARTAAIALLLAGAVFALLRGLDWEEALLLAVIAGLLVLYRDAFYRGGD
                     WRRFRPTPGWLALVLVTLVALTLVGFLAFRHVEYREDLWWDFAWDGDAPRFLRVSLLL
                     AVAAAALALDALLRRPAPLPAASEPIPDAVRRLLDACPDTQPQLALLGDKRFLVSDDG
                     DAFLMYAVAGRSWVAFGDPVGNPEAGRELVWRLAELADRAAGRPVFYALSPDLLPLYL
                     DLGLAILKTGEVAAVNLRNFSLAGKRRQDFRQALRRAERERITFDIVPKADVPELLPE
                     LKTVSDAWLAIKAGHEKGFSLGRFDERYLAEFDCAVLRQGGRVVAFANLLHGADRHEL
                     SVDLMRYRPDVTKGLMDMLFARIMLHGQEAGYQWFSLGAAPLAGLADHPLASTWNRVG
                     TLVYRRGDEFYNFAGLRAFKQKFDPTWTPQYLACHGGLGLPSVLMDIATLIAGSRAGL
                     IRR"
     gene            20747..20953
                     /locus_tag="P5558_07740"
     CDS             20747..20953
                     /locus_tag="P5558_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRY24238.1"
                     /translation="MYAVGLRELKNRLSEYVRLAASGERVLVTDRDRVVAELTAPRGG
                     TAETVPDVVIADLVRKGLATPRHP"
     gene            complement(21007..21171)
                     /locus_tag="P5558_07745"
                     /pseudo
     CDS             complement(21007..21171)
                     /locus_tag="P5558_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3034 a         7632 c         7297 g         3159 t
ORIGIN      
        1 gcgggaaggc gccggcgatc atgttgaaga gcgtggtctt gccggcgccg ttcggcccga
       61 tcagcccggt gatgctgcca cggacgaggc cgaaggtgca gccctggacg gcgcgcacgc
      121 cgccgaacgt cttgtagacg tcgacgatgt cgagaaggtt ctcgccggcc ttgccgcctg
      181 ccgccatgcc cgcccaccat tgatgccccg cccgaaaagc gacgggatac cggcgtttag
      241 cggcgatcca cccctgtcgc aagccggggc gggcgatcgg cctacacgat ggtgccgccg
      301 ccgtggcgtg gcgtgggtgc cggagccgcc ccgcgggaca gccggtaagc cagcttctgg
      361 cgcgccagaa gctcgatggc gcgatggtcg gcggtcagcg gcgcctggaa cacgacctcg
      421 acgaggctcg aaggatcgat cgcggaaacg cgctggaccg agcccagcct ggtgatctcg
      481 agcagaatct cgtcgggagc cggccgcatc ggtcgaatat accgcatcgg gtccgctgcg
      541 cgataggccc gctgcgcgat tgcccatcga cagttgcccc ctgcagcact aagtgatccg
      601 agggacatat atcggatcga tagaggtggc cgcgccggca tggtcggcga ggggggtgcg
      661 ggtgatgcag gatgtcgggt cgggacttgt cggcagggtg cttcgtcggg cgctggcgct
      721 tgggctgatg ctcggcctcg ccggcggcac ggcctcggcc cagcagggcc agcagggcca
      781 gcaggcggcg atggtgcagg tcgacgcggt gatcaccgag ccactggtgc agaccgtgcc
      841 ggtgctgggg cggatcgtca cccgccagga aggcccggtc gcatcccggg tcgaggggcc
      901 ggtggccgag atcggggtcg cggtcggcga ccgcctcgcc acgggtgatc tcatcgccgt
      961 gctcgacagg aagcggctgc agctcgattc ggccctcgcc gccgccgagc tcgccgccac
     1021 cgaggccgac ctcgcggccg cccgccgcga ggtggagctg ctcgagcagg agcgcgaccg
     1081 gctggtggcg ctgcagggct cggccgcctt ctcgcgtgcc cggctcgacg acaagatcaa
     1141 ggaggtccat gtcgcccaga gccggatcga ggccgccgcg gcgcgcctcg agcaggcccg
     1201 ggtcacgctc gactaccgca atgccgatct ggcggatgcc gagatccgcg caccctatcc
     1261 gagcgtggtg gtccagaagc acgtcagcgc cggtgcgcat gtgcgggtcg gcgacccggt
     1321 ggtgaccctg gtcgacgaca gcgccctcga ggtcgaggcc gacgtgccgg cggagcggct
     1381 cttcgagctg gagcccggca cgcccatcgc cttctcgatc gctggcgccg gccacgccgg
     1441 cgggcggcac gacgcggtgc tccgggcgat cgtgcccatg gagaacccgc tcacccgcac
     1501 ccgggccgtg cgcttcacct cggtgaacgg cgggctcgag gccgcccggg ccatcggcca
     1561 gagcgtcacc gtggacatcc cggtgggggc gccccgcgat gtggtcagcg tccacaagga
     1621 cgccgtgacg atcaatctcg gcacacggca ggtctacgtg gtcggcgacg acgacgtggt
     1681 acagccgcga gcggtagtgc tcggtgccgc catcggcaac cgcttcgagg tgctggaagg
     1741 cctggccccg ggtgacctgg tcgtcacgcg cggcaacgag cgcctgcgcc ccggccagac
     1801 ggtcgcgtac gggtcgccgg cgaacggcga tgccggcaag gaccagaagg gctagccggg
     1861 ttgaacctga tccgcctcgc catcgaccgc ccgatcgcgg tcatcgcggc cgtgctcatg
     1921 gccgtgatgt tcggctacgt ggccctcacc accatcccga tccagctcgc cccggacgtg
     1981 gtgaagccgg tgatctcgat caccacgagc tggccgggcg cggcgccggc cgaggtcgag
     2041 cgcgagatcg tcaacgagca ggaggaggtc ttcaagggcc tcgaggggct ggagcggatc
     2101 gagagcaagt ccgaggacgg ccaggcgacc atcaccctcg agttcagcct cgacaccgac
     2161 atggaccgcg ccctgctgct cgtcgccaac cggctgcagc gggtgccgag ctacccggcc
     2221 gaggccgacg agccttcgat ctcgacctcg agcgacgacg acagcgccat cgcctggttc
     2281 gtgatgaccc gggtcgaggg caacgaccgg ccgatcgaga gcttcggccc ctacgccgag
     2341 gacaccttcc aggatcgcct gcagcgggtg gcgggcgtcg cccgggtgga cgtcttcggc
     2401 ggggcggagc gcgagctgca ggtcatcgtc cggccggaga acctcgcccg ctacgggctc
     2461 accgtgcccg acgtcgtcga cacgctgaga agcgccggca tctcgctcag cgccggcgac
     2521 gtggccgagg gcaagcgccg ctacgtggtc cgcaccgagg gcgagctcac cacgccggag
     2581 gcggtcgaga cggtggtgct gcggtcgatc gcggacccgc tgaccggccg cctcgcgcgt
     2641 atcaccgtgg ccgacatcgc ggaggtccgc ttcggctaca gcgagaccgt ggcgaccatc
     2701 cgtgtcctcg gcgaggaggc gctggtcggc cgggcgatcc gcgagaccgg ctccaacgtc
     2761 atcgagacca tgaagggcat ccacgccgcg gtcgaggagc tcaacgccgg agccatgccc
     2821 aatgccggcc tgcaactgcg gcaggtctac gacgagaccg tctacatcaa cagctcgatc
     2881 gacctcgtca cccagaacat ctatgtcggc ggcacgctcg ccgccctcgt gctcctgctc
     2941 ttcctgcgct cgttcggcgc cacgctgatc gtctcggtcg ccattcccgt ctcggtcatc
     3001 gccagcttcg tcgccatggc cgccatgggg cgctcgatca acgtcgtctc gctcgccggc
     3061 atcgcctttg ccgtcggcat ggtggtcgac gcagcgatcg tcgtgctcga gaacatctat
     3121 cgcctgcgcc aggagggcaa gtcgccggcc gaggctgcct atcagggtgc cgcccaggtc
     3181 tggggtgcga tcctcgtctc ggcgctcacc accgtcatgg tgttcatccc gatcctcacc
     3241 atgaagctcg aggtcggcca gctcttccgc gacatcgcgg ttgccatctc ggtcgccgtg
     3301 ctgctctcgc tgctggtcgc catcaccctc atcccggcca tgtcgaagcg cctgctcggc
     3361 ggcgccgacg gcagcctcgt catgcgccgc atcccggtgg tcgacgacct cgccggtctc
     3421 ttcgtccggg ctgtcctctg gacgacccgg cgcgtctgcg cctcggtgcc gctctcgctc
     3481 gccgtcgtcg ccagcgtgac cctggtcacc ggctacctcg cctgggccaa cctgccgaag
     3541 ctcgaatatc tgccgcaggg caaccagaac ctcgtcttcg gcatcgtcct gccgccgccc
     3601 ggctacaatc tcgagacgat gacggacatc gccaccgaca tcgagggcgc gctgcgcggc
     3661 ctctgggcca gcgagaccgg ccccgagccc accgccgacg gcatccccaa gctcgaggag
     3721 ttcttcttcg tcgccttccg cggcaacatg ttcgtcggcg gcagctcggc cgacatggag
     3781 cgcgccgggg cgctgatcgg accgctgaca gcacccgtct tccgcgagcc gggcaccttc
     3841 ggcttcttca gccagccctc gatcttcggc ggcggcatcg gcggcgggcg gaccatcgac
     3901 ttcgacatct ccggccccga cctcgagacc gtgctcgcca tcgcccagcg cgccaccggg
     3961 atgatcgccg gcctcctgcc gcgcgagcag ggccaccagt tccgccccga ccccggcctc
     4021 gagctgggtg cccccgaggt ccgcctgatc cccgaccgcc tcaggctcgc cgatgccggc
     4081 gtctcggcgc gcaacttcgc gctcagcgtc gacgccttca acgacggcct cagggtcgac
     4141 gagatctcgg tcgacggcga ccagatcgac ctgacgctca agggccccga gggcaatgcc
     4201 gagcgcaccc agggcatcga cgcgctgccg atcgtcaccg ccgagggcca tatcctgccg
     4261 gccggctcgc tcgcccggat cgaggtgacg gcggggccga ccgagatccg ccatgtcgag
     4321 cggcagcgca cggtgaccct gaaggtcagc cccgccccct ccctgccgct cgaggtggcg
     4381 ctcgacatcc tgcagaacga ggtggtggcg ccgctcgagc agcagggcct gccgcccggc
     4441 gtgcagctcc gcctctccgg caccgccgac cagctcaccc agaccttcga cgccatggtg
     4501 ctcgacctgg cgctcgccgt ggtcatcgtc tacctggtca tggcggtgct cttcgagagc
     4561 ttcatctacc cgctcgtgat cctcttctcc gtgccgctcg ccgccgccgg tggcgttgcc
     4621 gggctcttcg tcctcaacct ctacaccttc cagccgctcg acatgctgac gctgctcggc
     4681 ttcgtcatcc tcgtcggcat cgtcgtcaac annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nctcgacatg ctgacgctgc tcggcttcgt catcctcgtc ggcatcgtcg
     4861 tcaacaacgc catcctcctg gtccaccaga ccctcttcga gctgcgcgag cacggccgcg
     4921 caccgctcga cgcgattgcc gaggcggtgc gcaaccggct ccgcccgatc ttcatgtcca
     4981 ccatgacctc ggtcatgggc atgctgccgc tcgtcctctt ccccggcgcc ggctccgagc
     5041 tctatcgcgg cctcggctcc gtcgtcgtcg gcggcctctc gctctcggcc gtgctgaccc
     5101 tcctgctcat cccacccttg atggcgctgg tcctggtgcg ccagcagcgc cgggcggcga
     5161 cgcctgcgat ggtgaagggc gctgctgcgg caccggccga gtaggcagcg ttaatggccc
     5221 ggtgcgactc cggtatgctg aagcttcggt tagccagccg cgttaacccc gggccttccc
     5281 gtctgccgta atcttcgagc gcaggtcgcc agcctgatgc ctcgaggaga gcagatcatg
     5341 gtcgccgccg cccagccggc tcccgccccg gtgccctcgc cgatcgacat tgccgcgccc
     5401 gtcgccaggc ccgaaggcga cgaccgcaca cggctcgccg cagcttccgg cgaggcggga
     5461 acggccgccg cggccaaccc gctgccgctg gccgtggccg aagcgatggt gcaggaggag
     5521 gccgcggccg aggcgacgcc acgcggtgcc gacgccccgc ccgaggcaga ggcggcaccc
     5581 gaagcggcgg cagccggcat cgagacgaca tcgccggccg aggcggtcga gcccggcgcc
     5641 ggtccggcca atggcggcac gcggccaccc gctggcggtg gcgccggctt cacgacctac
     5701 gaccagggcg atatcggcga cgggctggac ccgacgggcg ccctggggcc gacggcgctc
     5761 gaccatgggc tggtcaccaa cgttccgttc tcgggacact acggcaacga cgcatccggc
     5821 cggggtgcgg gcgagcctgg cggcgattcc ggtggcgacg ggggcggggg cccgggcgat
     5881 ccgcaggcca accgcgcacc cgtggcctgc gacgacagct tcacggccgg cgagaacctg
     5941 gttcgcaaga tcgcgctcgg cgacctcctc gccaacgacc acgacccgga tggcgacccg
     6001 ctcagcctcg tctcggtgca gggcgccgaa aacggcacgg tgcggctggg cgccgacggc
     6061 tttctctatt tcacgccgac ccacgactac agcggcgagg ccggcttcag ctacaccatc
     6121 agcgacggca agggcggcct ctcgaccgcc gaggtccggc tcgacgtgcg cgccaatacg
     6181 gacccgcatg ccaccgacga caacgtcaac accaccatga acacgctcaa ggtcatcgag
     6241 ccctcgaacc tggtcaacga cgatttcgac gccgatggcg acgcgctccg cctcgtctcg
     6301 gtgcagaacg cgcagcacgg cgaggtctgg atcggccagg gcggcaagct ctatttccag
     6361 cccgaccagg gcttcgtcgg cgaggccggc ttcagctaca cgatcaccga tgatcggggc
     6421 ggcatcgaca cggcacgcgt cgatgtccac gtccacgaaa agggctggca gaagggcgag
     6481 cccggcaacc atgcgccggt cgccaccgac gacaacctca cggtccggat ggacacgctc
     6541 aaggtcgtgc agcagtccaa cctcaccggc gacgacttcg atgccgacgg cgacgcgctc
     6601 cgcatcgtct cggtccagcg ggccgagaac ggcacggtct ggctcggcca ggacggcgag
     6661 ctctatttcc gccccgacga gggcttcgtc ggcgaggcgg gcttcagcta cacgatcgcc
     6721 gacgaccggg gcgggctgga cacggcgcgg gtgaccgtcc aggtcgcggc acccgagggt
     6781 ggcatgccgg ggccggaggg gtgcggctgc ggcggcgagc aggtggccaa tctggtcccc
     6841 ccggcggagc atgtcgtctg agacattggc gcgcgggacg gctcgcacgg gccgtctcgc
     6901 ctcgcttccg atcggctgct accttcctcg ccatgcactc ctgcggcgcg aggccagccc
     6961 gtgatcgaaa ccccctatct cctcttcctc ggcgacgtcc ccgaccagct cgccgccaag
     7021 accgcccagg gcgtcgtgca ctggcgcccg gaagcctgcg ccggccagct ccgcctgccg
     7081 ggctgcaagg ccgatctcgg cctgcccgag ctctcgatcg acgccggtgc caaggctggc
     7141 gtgcgcacct tcctcgtcgg cgtcgccaat gccggcggcg tgctgccgcc gcactgggtc
     7201 gccaacatcg tcgaggcgat cgaggccggc atggacgtcg cctccggcct gcacgtgcgc
     7261 ctcggcgacg tgccggagat cgctgacgcc gccaggcgga agggcgcacg cctgcacgat
     7321 gtccgccacc cgacccgcac cttcgcgacg ggcaagggca cgaagcgggc cggcaagcgc
     7381 atcctcgccg tcggcaccga ctgctcctgc ggcaagatgt acacgacgct cgccctcgag
     7441 cgggagatgc gggagcgcgg catgcaggcc gacttccgcg ccaccggcca gaccggcatc
     7501 ctgatcaccg gcgagggggt ctcgatcgac gccgtcgtcg ccgacttcat ctcgggtgcc
     7561 gtcgagtggc tctgcccggc caacgacccc gatcactggg acctcgtcga gggccagggc
     7621 tccctcttcc acccttcctt cgccggcgtc agcctcggcc tgctgcacgg tgcgcagccc
     7681 gatctcctgg tcctctgcca cgagccgacc cgcacccaca tgcgcggcct gccgcaccag
     7741 aagctgccgg gcctgaagga atgcctcgag cgcaacctcg aggccgcccg gctgaccaat
     7801 cccgatgtcc aggccctcgg cgtcagcatc aacacggcag cgctcaacga ggccgccgcg
     7861 cgcgactaca tggccgaggt cgagcagcag ctcggcctgc ccacggtcga cgccttccgc
     7921 accggcgtcg cccggctggt ggacgccctg tcgtgaccaa gaggcggctg gacatcgcgg
     7981 tggagcgctt tcccctggtc aagcccttca ccatctcgcg cagcagcgtc accgagctcg
     8041 agctggtcac ggcgaccatc accgagggcg agcatgcggg gcgcggcgag tgccggccct
     8101 atccccgcta cggcgagacc accgacggtg tcgtcgagga catccgcgcc cgggccgggc
     8161 tgatcgaggc gggcgagggg tgcgcggccc tgctcgaggc gatgccggcc ggcgcggcca
     8221 ggaacgccct cgactgcgcc ttgtgggacc tcgaggcgaa gctcgctggc cgcccggtgc
     8281 atgacctcct cggcctgccg gcgccggcgc ctgtgctcgt cacctacacg atcggcctcg
     8341 actcgccggc cgccatggcg gccgaggcca aggccgtcag ccggccgctg ctcaagctca
     8401 agctcggcgg ctcggacgat ctcgagcggg tgcgggcggt gcgtgctgcc gtgccggaga
     8461 cgcggctgat cgtcgatgcc aacgaggcct ggtcgcagga gatggtggtg gcctggatgc
     8521 ctgagctcgc cgccatgggc gtcgagctga tcgagcagcc gctgcacgcc gagcgcgacc
     8581 gggcgctcga gggcctcgcc cggccggtgc cgctctgcgc cgacgagagc gggcacggca
     8641 tcgaggatct cgacgcgctg gcccccctct acgacttcat caacatcaag ctcgacaagg
     8701 ccggcgggct gaccgcggcc ctggccctcg ccgacgcggc cgaggcgcgc ggcttccgcc
     8761 tgatgctcgg ctgcatgatg gcgacctcgc tcgccatggc gccggctttc gtgctcgccg
     8821 gccgcgcccg cttcgtcgac ctcgacgcgc ccctgctgct cgcccgcgat cgcgagcacg
     8881 cgatcaccta cgacggggcg atgatgcagc cgccgccgca ggcgctttgg ggttgaaggg
     8941 ggcggggctg agcaggctca ggccagccag gtccggtagt cgctgaccag cagccgggcc
     9001 ggcggctcgt cctcctcgat ctcggcgaag cggccgacgg gctcgcggaa gatgttgtcg
     9061 gtctcgtcgt cgttgacggt cgagacctcg ccgatcagga cgtcgccgcc cttcgtccag
     9121 agcgcgtgcc agtcgccggg tgccagcgtc acgctctggc cgggcgccag ctcgaggcgc
     9181 tcgcccggcg catagtcgtg gcggatgccg tcgcggtgca cggtgccgcc ccggtccgcg
     9241 gcgaagccgc cctgctcgtc cgagccgaag agctcgacca cgagcgtcgc accgccgcgg
     9301 ttgatgatgt cctcggcctt gatgaaatgg gtgtggctcg ggcagcgctg cccttgcctc
     9361 agaacgagaa gcttctcggc atagcacatg ccacgaccgc gctggaggtc ggcgagccgg
     9421 ccgttgcgca gggtgaagac ggccaggccc acctcgtcga accggcccgt gccgaaatcg
     9481 gtgatgtccc agccgcagcg ggcgtcgatg acatggcgtg ccgtctcccg ccgggctttc
     9541 agctcatccg gtgtccagtt ggcgaagggc ggcaggacga agccgtggtg gcggatcatg
     9601 gcgtccgcct cgacgatgat ggcgttgacg cgcgagcgct tcatcctggt tctcctgcct
     9661 gctcgccgct cgtctcgagg aattccagca ccatggcggc ggtccaggtg aaccggtcgc
     9721 cgccgcaggg ctcgccggtg agcgggtcgt aatattcggc gaagccgctt cgcgtgatga
     9781 gatcgatgct gtcggcccgg atgcgcgcgg cgatggcggc ttcgcccgca gcggccagcc
     9841 cgtcggcgat catgtagttg acgatcagcc agacggggcc gcgccagtag cgcttggcat
     9901 cgaagcgggg atcggccgga tcgtggctcg gcacgagatg gcggctgcgg ccggccagcg
     9961 tctcgatgcg gtgcccgagc gcccgggcgc gatcctgcgg gatcggggcg aaggccgcga
    10021 gcaggccgcc gacggagcgg ctcccgacca gccggcccgt cgtccggtcg cgcgccaggt
    10081 actggccatc ggtcacgctc cagaggcccg ccatggccac gaggccgtgc tcggcgaaag
    10141 cgtggttgcg ggcggccagc ttggcatcgc cgagccgctc cgccaggacc gcgagatcgc
    10201 ggcaggagcg gatcaggatg gcgttgaagc cgggatcgac cacctggaag ggcgagtcgt
    10261 cgtgcagccg cgcattgtcc cagccgaggc cgcgaaagcg ctcgaggagc cagacgaagc
    10321 ggtcgtactc cgcgttggtc ggccgatgcg cggcatccgc atgctgcagg tcgcggcggg
    10381 tgtagggctc gatgccgtcg gtcggcaccc gcgccagggc ctcgtcccag tcgatcgagt
    10441 tgtcgcggcc ggattcccag gggtggatga tggcgaccag cccctcgccg gccgggtcgc
    10501 gatgggcgaa gaaccactga tgccagcggt cgatcgcggc cagcagcggc cgggcccgtt
    10561 ccgccgccat ggccggatcg cgcgcccgct cgaagaggcg gcgcacggcg aagccggcga
    10621 cggcgggctg ggtgatgccc gaggtcggca ccggacgatt ggtgcgccag atctccgggc
    10681 cggggaaata gccctcgtcc ggcacgtgga agacgatgtg cggcaccatc ccgtccggcc
    10741 actggtgggc gaggagcgtc tcgatctcgc gccaggcgcg gtcctcgtcc tgatggccga
    10801 ggccgagcgc cgtcaggcac gaatcccagt tccactggaa cggatagaga ccccgggtcg
    10861 gcacggtgta gccgccgcgg tcgttctccc gcaggatcgc ttgcgcgttc tcgaccaggg
    10921 tcgcgcgatc ggcggggtcc atgggcgctc ccggatcagg ccccggcgac gctgtagagc
    10981 tcggggtcgt agacatagcc ggcctcgtcg agggccgcga ccagggcctc gcccttggcg
    11041 ccgtcgagct cgaccatggg cggccgcacg atccgccaca cctcgtcgtg ctggccatcg
    11101 gcgatgacat gcttgatgcc cggcaccagc gggtagctgg tgagcaggtt gcggacgtcg
    11161 ctcaccagct tcatgccggc ctcggcggcc gaccggtcac cgtcattgaa cgccttgaag
    11221 accctggcgc tgggcccggc cgcgagattg gctgccgccg agaaggtgcc ggccccgccg
    11281 gcctcgagat tgcgcacgag atggccgtcg gcgcccgagt agatggtgag ctcggggaag
    11341 ttgctgatca ggctcgccgt gtgctcccag tcgccggagc tgtccttgat cccctcgagc
    11401 gtgtcgggat agcgtttcag cagccgctcg atcaccggat gggtcaccgg cacgcccgac
    11461 atctgcggga aatgatagag cagcaaggcg aggcgcggct cggccagggc gtcgatcacc
    11521 tcggcgaacg cccgatagag accctcgtcg ctgttgttct tgtagaagaa gggcggcagc
    11581 atcaggaggt tggtgacgcc gagaccgagc gcatgcctgg ccagcgccag cgtgtcgctc
    11641 ctggcgcagc agccgatgcc gaggatcatc cgctcgaccg gcaggccggc cgcgacatag
    11701 gcctcgagcc cggcctgccg ctcggcgacc gagaaggcct gcgtctccga cgtggtgccg
    11761 aacaccccga gcccggtgca gccctggcgc agcagccagc gggcatgccg gcaatagcgc
    11821 tcgacgctga tggcgccgcc cggctcgacc ggagtcacga cggcggcgta gattcccttg
    11881 gcaggcatgc tccgaactcc cctcgaggcg aatttccagc ccttgctacg gcagccgggc
    11941 gggcaacggc aaggccggtg ccgtggcagc cctggatcag tcgacggttt cggtctccgc
    12001 cacgccgtag aactggccgc gctcgagcca accgcgccgg ccgtcgatct cgaccaggca
    12061 ccaggcgccc tcgcagctca ggaggtcgcc gatcaccatg ggctcggcca ggagcacgac
    12121 ccgcgcatcg ctgcgcggcg tgcggcggag cggccgcacg tcgccggtga ccagcaccgt
    12181 gcgccggctg gagagcaggc tcgcatggat ccagccggtc tcgccctcgt ggtcgacgat
    12241 ccgccgccag tcgaacgatt cctccacgat ctgcaccgga atgccgcgct gcgtgaagac
    12301 ccagcgaatg gggaaggtct cggccggccc ggtacggacg ttggtctcgc tggcggcgag
    12361 ggagacgaag cgcggcaggg gcaggccggt cgactgggcc gctgccggcc ccgcgccggc
    12421 ggcgagggcg agggtcagga gcagggcggc gagcagacga cgggcgaagg gggagaacaa
    12481 ggcaacgcac cacttcgggc agggattctc ggagccggcg tcgacctcga agcgcatgcg
    12541 cacgccggct gcccgccctc tatgagccca ctctatagga aggccgggcc cgccgatcca
    12601 agcgcaaagt gaaaagtggc cacggagcag gcgtttgtga gcggtcgggc tttctgctag
    12661 gagccggttc gagcggccgt gcgccgggcc gtttcgggcc ggcgccagga caggagatcg
    12721 aggaatgccc cgcaagcggc ccctggtggt ggtcacccgc aagctgccgg acgtcgtcga
    12781 gacacggatg atggagctct tcgacgcccg cctcaatgac agcgacacag ccatgacccg
    12841 ggcagagctg gtcgaggcgg tcaaggcctg cgaggtgctg gtgccgaccg tcaccgaccg
    12901 gatcgacgcg gccctcctcg cgcatgccgg cgagcagctc aagctgatcg ccaattacgg
    12961 caccggcgtc gacaatatcg acgtcgaggc cgcctacaag cgcggcatca ccgtgaccaa
    13021 cacgcccggc gtgctgaccg aggacaccgc cgacatgacc atggcgctga tcctcgccgt
    13081 gtcgcgccgg ctggtcgagg gcgagcgcct cgcccgctcc ggcgaatggg gcggctggtc
    13141 gcccacctcg atgctcggcc accgcatcca cggcaagcgc atcggcatcc tcggcatggg
    13201 ccggatcggc tcggccgtcg cccggcgggc gcaaggcttc ggcctcgccg tccactacca
    13261 caaccgccgc cgtgtccacg aaagcgtcga atccgagctc gaggccacct actgggaaag
    13321 cctcgaccag atgctggcca gggtcgacat cgtctcggtc aactgcccgc acaccccggc
    13381 caccttccat ctgctcaacg cccgccggct caagctgctc cgccgggaag ccatcgtcgt
    13441 caacaccagc cgcggcgagg tgatcgacga ggcggcgctc acccgcatgc tgcgcgacgg
    13501 cgacattgcc ggcgccgggc tcgacgtctt cgagcacgag ccggcgatca acccgcggct
    13561 ccgccagctc gagaacgtcg tgctgctgcc gcacatgggc tcggccacgc tcgagggccg
    13621 gcgcgacatg ggcgagaagg tcatcatcaa catcaagacc ttcgccgacg gccaccgccc
    13681 ccccgaccgc gtcctgccga gcatgctctg atcatcggcg atcaagctgg acaggcgatg
    13741 acagcactgt cacttgccgc ggtccggcct cccggctgaa aagagcctcc cgcgcgaacc
    13801 agcctgaaag gcggttgccc cttgttcacg tctttctttc cccagccgaa gctgttcttc
    13861 ctctccgtcg tcctctggtc ggcggtggca acggcgctgt ggtacggtgg tgctgacggc
    13921 tggggcgccc tgatcggcct cgggaccacg cccgagggga ccgagacggt gctcgggctc
    13981 ggctatttcg tcacgcccga gttcctctgg ttctacatct actatgccat cgtcgtggcc
    14041 ctcttcgccg ccttctggtt ccgcttcgac ccgcatccct ggcagcgctg gtcgatcctg
    14101 ggctcggcgc tgatcctctt catcacctat ttcggcgtgc agatctccgt cgccgtcaac
    14161 aactggcgcc ggccgttctt cgacgacgtc cagcaggcgc tgggcgagaa ttcgaccgtc
    14221 acccaggccg acctcttcgc gctgctcggc atcttcgccc agatcgcctt tctctccatc
    14281 ttcatcttcg ttctctcgcg cttcttcgtc agccactgga tcttccgctg gcggaccgcg
    14341 atgaacgact actacatggc ccgctggcag cgcctgcggc acatcgaggg tgcctcgcag
    14401 cgcgtccagg aggacacgat gcgcttcgcc ggcatcatgg agggcctggg cgtctcggcg
    14461 atcgacgcgg tgatgacgct catcgccttc ctgccggtct tgtgggagct ctcgaaatac
    14521 gtcgaggagc tgccgctggt cggcgccatc ccggcgccgc tcttcgtcgc cgccctcgtc
    14581 tggtcgctct tcggcaccgg cctgctcgcc cttgtcggca tccgcctgcc gggcctcgag
    14641 ttccgcaacc agcgggtcga ggcggcctac cgcaaggagc tggtgcacgg cgaggaccag
    14701 gccgaccgcg cccagccgat gaccgtgcac gagctcttcg gctacgtgcg gcagaactat
    14761 ttccgcctct acttccacta catgtacttc aacgtcttcc gctcgctcta cctgcaggcc
    14821 gacaacatct tcgtctacat cctgctcatc ccgaccatcg tcgccggcgc cgccgtcggc
    14881 ttcaccttcg gcgtgctgca gcagatcctg accgccttca accaggtctc caactcgttc
    14941 cagtatctcg tcaattcctg gacgacgatc gtcgagctgc tctccatcca caagcgcctg
    15001 atggccttcg aggccgcaat cgaggacaag gagctgcccg acatcgacaa ggaatggctg
    15061 gaaatccacg gcgccggcga cgacggcggg gtgcgcaccg tcggctcggg catctgagcg
    15121 gccgccgggc cgagaaaccg gcagcgccgc cttcccgccg accgggaacg gctggtcgag
    15181 gcgctgcagg cggcggggct cggccgaggc tcgccgatca ggtccccgac ccggccggcc
    15241 ggcgctcgat ggtgatgcgt cggtggtaga acgggtcctg cacaggtggg ccgagggcga
    15301 ggacggtggt ggcttcgagc tcgtgggcga agaggtcggc caggagctcg cggcggtcgg
    15361 ggtcgagggc gctcatcggc tcgtccatga cgacggcggc gggccggtgc aggaggaggc
    15421 gggccgccac caggcgctgc tgctcgtcgg tgccgagctc ctggtcccag cgacgctcgg
    15481 tgtcgagaag cggcacgagg cgggcgaggc cggtgcgctc gagggcggcc ccgggatcgg
    15541 tgtccgtcga cgcgggcgaa tcgggcaggg ccgggtaggc gagcacggcg cggagcgagc
    15601 ccagcggcag ataggggcgg cccgggagga aggcgagccg gtcgcgcggc ggcaggtgga
    15661 ccgtgccgga gccccagggc cagaggccgg cgagcgcgtg gaagacagcg ctcttgccca
    15721 cgcccttggg gccggtgagc agcaggcgct cgccgggtgc cagctcgacc aggggctccg
    15781 ccagcaccac ctcgctgccg ggcagggcga cgcggaggtc ggcgagggtg atgcggggct
    15841 cggtgtgctc gacataggtg atgcggcctt cgccctcggc gagcgtgtcg aggccgggca
    15901 ggatctcgcg cagggcgacg acgcgaaaga gggtggcgcg ccagtcggcg atcgccggga
    15961 aatagtccac gaaccagcgg agcgccgcct gcacctggtt gaaggcgccg acggtcatca
    16021 tcagcccgcc gaaggagagc tggccctgga aatagcccgg tgccgccgcg agaaccggga
    16081 cgatgaggcc gagccagcca tagcccgagg tgatccaggt gaggcgcgcc agcgcatagg
    16141 cgaggcggcg ggtgatggcc accaccgcgt cgaccggctg gtcgagcagg cggcgctcgc
    16201 ctgccgcacc gtcgtggagt gctatcgcct cggcgttctc gctgatgcgg accagggcga
    16261 aacggagctc ggcctcgcgg gcgtagcgct cggcgttgag gccgatcatc ggccggccga
    16321 cgcgccaggt gagccacgag ccgctgacgg cataggcgag ggcgcaccag acgaggtagc
    16381 cgggaatgtg cagctcgctt tcgcccagcc ggaagacggc gccggcggag agcgtccaga
    16441 gcacaccgag gaagcccgcg agcaggaggg tcgagcggag caggccgatg ccgagcgtga
    16501 tggtgagctc ggtgagctgg cgggcatcgg cctgcaggcg ctggtcgggg ttcctcgcga
    16561 tgtcgccctg gaagccgagg agccaggggc ggcggccggt gacccactgg tcgagcaggt
    16621 cgcgggtcag ccattcgcgc aggcgcacct cgagcagctc gcggaaccag gtctcggcga
    16681 cgacgaagag gagcagcacc aggacgatgg cgaagaagat cagtgtctgg tcgaggagtg
    16741 ctcccggcag gcgctgctcg agggcgtcga agaaatcgcc ctgccaggcg ttcaggcgga
    16801 tctggcccag catgttgagg cagatgacgg cgacgaggcc gaacgtcagg aggaagcagg
    16861 cacgccgata gggcgagcgg cggagcgcgc cgaaaaggac gcggagctgg cggaaggcat
    16921 cgcgaaagcc gatctcgggc ggcgggccgc ctgcggatcc ggtccgggcc gagagatcgg
    16981 gctgcggcgg caagcgggct cctcccgcgg gcacccctgg cagcttgggg ggcgggctcg
    17041 ggcagtggtc gcggctgcat agccggcggg tccgcggctg tcgatggcgg gtggaggctg
    17101 cgcggacggc gcgccggtga tatcaactcc tggcgcgtga gcatcggcag tcgggaggca
    17161 gggatgacgg aggaacgcta cgaggtcttc gcggtgcgct acgggaccgt cgcggcgcgc
    17221 acccggcgcg agaacttcat ggggctggac ccgcacgaga cggcacccat gccgctcgac
    17281 tactatgtct gggtggtcag gaacgaggct cggacgatcg tcgtcgacac cggcttcgac
    17341 cacgcggagg ccgcgcatcg cggtcgccgg ttggaacgcc tgccgcgcga agggctggcg
    17401 ctggtgggcg tcgacacgct caccgtcgag gacgtgatcg tcacccacct gcaccacgac
    17461 catgccggca cgctcggcga cttcccggcc gcccgcttcc acctgcagga ggcggagatg
    17521 gcctacgtga cgggggcctg catgtgctac ccggcgctgc ggatgccgtt cagcgtcgag
    17581 catgtctgcg agatggtgcg caacgtctat tccggccggg tggtcttcca cgacggcgac
    17641 gccgaggtgg cgccgggggt gacggtgcat cgcctgggcg ggcactccaa gggcatccag
    17701 tgcgtgcgcg tccagaccga gcggggtccc gtggtgctcg cctcggacac cgcgcatttc
    17761 tacgagaacg tcgagcaggg caagcccttc atcatcaccc acgacgtcga ggcgacgctg
    17821 cgcggctatg atcgcctgcg gaagctggca gccagcccgg cgcatatcgt gccgggccac
    17881 gaccccctcg tgctgcagcg ctatcccgcc ccggacgagc ggctcgccgg catcgtctgc
    17941 cggctcgacg tgatgcccca aggctagcat ggccaccgct gccggcgagg ctggcgcgaa
    18001 gccgggatcg cgctggcatt tgccgcacct cgcccccctg gtcggcgtgg cggtcgtggc
    18061 cctggccgcc tatgtcatcc atcgggtctc ggccgaggtc catctggccg acatcgaggc
    18121 ggcgctcgcc gccacgccgc tccgctcgat cctgctcggc ctgctcctca ccgccacgag
    18181 cttcctcgcc atgtcgctct acgacgtgct gggcgccgag caggtggccc ccggccgggt
    18241 gcccaaggcg atcgccgcgt tcgccggggc ggcgagcttc gccgtcgcca acgcggtcgg
    18301 cttccacgtc ctgaccggcg tgccgatccg ctaccggctc tatgccgggg ccgggctcga
    18361 catggcccag gtcgggcaca tcgtcggcaa cgcgctggtc gccttctggc tcggcgtcgc
    18421 caccctgctg ggtgccgcca tgctgatcga tccggtcggc gtgccgcttc tcgacaggct
    18481 cgacccccgg ctcgaccggg tgctcggtgc ggtgatcctc gccgtgatcg ccggcatggt
    18541 cctgtggctc ggccgcaacc cccgcacgct tgccctcttc ggctggcgcc tggtcctgcc
    18601 gggtgcggcc ggcatgctgg cgcggatcgg gctcggcgtg gtcgatgtcg gctgcgccgc
    18661 cgccgccctc tacgtgctgc tgccggccga cgtccagccc ggctacgcgg tcttcctcct
    18721 cgtcttcctc accgccatcg tcgccggggc ggcgagccat gtgccgggcg ggctcggcgt
    18781 gctggaggcc acggtgctgg tcggcctcgg cgccggcacc aaccccgacg tcatcgccgc
    18841 cctcgtcgtc ttccgcgtcc tctactacct cctgccgctc gccctggccg ggctgacgct
    18901 cgccctcttc gagggacggc gcgggctcgc cccggtcgcc ggcaaggcgc tgcgcgtcgg
    18961 ccggccgctg gtcccgccct tggccgcggg cctcgtcttc atgggcggaa tcgtgctgct
    19021 gctgaccggc aacctgccgg cggaaggcag ccggctcgac cggctcggcc acctcctgcc
    19081 tttgcccttc gccgaggcct cgcacatggc gtcgagcctc gtgggcctcc tcctgatcgt
    19141 cctggcgcgc ggcctcctcg cccgcatggc gctggcgcgg acggccgcca tcgccctgct
    19201 gctcgccggt gcggtcttcg cgctgctgcg cgggctcgac tgggaggagg ccctgctgct
    19261 cgccgtcatc gccggcctgc tcgtcctcta ccgcgacgcc ttctatcgcg gtggtgactg
    19321 gcgccgcttc cggccgaccc ccggctggct ggcgctcgtg ctggtgaccc tcgtggccct
    19381 gacgctggtc ggcttcctcg cctttcgtca cgtcgagtac cgcgaggatc tgtggtggga
    19441 tttcgcctgg gacggcgacg ccccccgttt cctccgcgtg agcctgctgc tcgccgtggc
    19501 ggcggcggcc ctcgccctcg acgccctgct ccgccgccct gccccgctcc cggcggcatc
    19561 tgagcccatt ccggacgccg ttcgccgcct gctcgacgcc tgcccagaca cgcagccgca
    19621 gctcgcgctg ctcggcgaca agcgcttcct cgtctcggac gacggcgatg ccttcttgat
    19681 gtacgccgtc gccgggcgga gctgggtggc gttcggtgat cccgtgggca atcccgaagc
    19741 cgggcgcgag ctggtctggc gcctcgctga gctcgccgac cgtgcggccg gccgccccgt
    19801 cttctatgcc ctctcgccgg acctcctgcc gctctatctc gacctcggcc tcgccatcct
    19861 caagaccggc gaggtggcgg ccgtgaatct tcgcaacttc tccctcgccg gcaagaggcg
    19921 ccaggacttc cgccaggcgc tccgccgcgc cgaacgcgag cgcatcacct tcgacatcgt
    19981 gccgaaagcc gacgtgccgg aactcctgcc cgagctgaag accgtctccg acgcctggct
    20041 cgccatcaag gccggccacg agaagggctt ctccctcggc cgcttcgacg aacgctacct
    20101 ggccgagttc gactgcgccg tgctgcggca agggggcagg gtcgtcgcct tcgccaacct
    20161 cctccacggc gccgacaggc acgagctctc ggtcgacctc atgcgctacc gcccggacgt
    20221 caccaagggc ctgatggaca tgcttttcgc ccggatcatg ctccacggcc aggaggccgg
    20281 ctaccagtgg ttcagcctcg gcgcagcacc tttggcaggc ctcgccgacc acccgctcgc
    20341 ctccacctgg aaccgcgtcg gcaccctcgt ctaccggcgc ggcgacgagt tctacaactt
    20401 cgccggcctc agagccttca agcagaagtt cgaccccacc tggacccccc agtacctcgc
    20461 ctgccacggc ggcctcggcc tcccctccgt cctcatggac atcgccaccc tcatcgccgg
    20521 cagccgcgcc gggctcatcc ggcggtgagg gggcagggac agcaaagaaa aagcggcaag
    20581 gcgcggcctc accgccggcc cctctgttct ttttgggata aaggggtcag cgactgtctt
    20641 ggtctgtcaa gctgcttacg ctgccccatg gcgttccgtc cctgagcatg gcgttgatga
    20701 tgacgaggag cttgctcctg cagccacacg cggccacatt gaaacgatgt acgccgtggg
    20761 cctccgcgag ctgaagaacc gtctctccga gtatgtccgg ctcgcggcat cgggcgagcg
    20821 cgtcctggtc accgaccgcg accgcgtggt ggccgagctt accgccccgc gcggcggcac
    20881 cgccgagacg gtccccgacg tcgtgatcgc cgatctcgtc cgcaagggcc tcgctacgcc
    20941 acgccacccg tgacgcgcga gcgcgcgcta ccgccatctc gtggcgaccg gaagctcgcg
    21001 gacatccagc ccggcctcgc ccgctgcatc gaaccgggcc agccacttgt agccggtctt
    21061 ccggctcacc ccgaaccgac ggcagagcgc cgcccggttg gcatccccgt gcctcgccaa
    21121 tgtcacgaat tccatacgca gagacatctc gtccaccgat cgccaagcca tgcgaaacct
    21181 cccgaccgcg tcaggtcgaa agtgtcaccc atgtcctcgt ac
//
LOCUS       DAOTFI010000058        21190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2986_length_21190_cov_6.111947, whole genome shotgun sequence.
ACCESSION   DAOTFI010000058 DAOTFI010000000
VERSION     DAOTFI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21190
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_21190_cov_6.111947"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1206)
                     /locus_tag="P5558_07750"
     CDS             complement(46..1206)
                     /locus_tag="P5558_07750"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY24239.1"
                     /translation="MGAIRKEIEDLELSGISKVALGALGDPEVIPLWFGESDIVTPAF
                     IRDAAKAALDSGQTFYSYSRGTPALRAGLKTYLDRLYGIDLHPDRITVAGSTMLSVVV
                     AAECLLDRDDEIVLLGPYWPNIRVAARLMGARSVDVRLRAGERRWELDLGEVARAITP
                     RTKAVYVNTPSNPTGWIMSAEEGSALLDLCRHNGLGLIADEVYHRNVFHGRDPAPSFL
                     ELAGPEEPVFVLNGFSKAWAMTGWRLGWMVAPQHLVEPMAVLAECNNTGATVFAQAGG
                     LAALEQGEEFVRSFVARTRINRDLVMELIGSHPKVTLPEPEGAFYAFPRIEGLTDSLA
                     FAREVLQRCKVGIAAGYTFGAGNESHVRLCFAIRTERLRLALERLRGLLDEL"
     gene            complement(1249..2709)
                     /locus_tag="P5558_07755"
     CDS             complement(1249..2709)
                     /locus_tag="P5558_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY24240.1"
                     /translation="MRLRTKLLVVMLPVVLAGLTLQWLVSSYEQGRQWAGQHEELLTH
                     LLDNAALRIVQDRHRVLETYALENVPAYRERYETAALAELAELASRSGTELFVLDGQG
                     DVLLPAGLVLDEASPLPESSAAAFVQAIGGTSYLLAARHFEPWDWTLVGGYPADARNL
                     AIRRVNLAVTSTLLLTGFLMTVSLFVGLDRLVVRPIRLLEENARALARRRRPLDLQLA
                     GKDELSELAAEMEAMATAIATHTAELERSNAELDHFAAAVSHDLRAPLRAIVNIAGWL
                     EADAGPLPQAALDHLDRLRDSAGRMDAMLLDLLRYARASQVAGKADQCAVRELIEAQL
                     ALLAPAKPVTLEIDAPVATLITPEAPLALVVRNLMDNAIKHHDRGAVQLKVDVCRGDR
                     RAIFRVTDDGPGISPAEHDRIFDIFRIRDGQSSGGSGIGLALVRRIVQRLGGTMRVES
                     DGTTRGTTVVFDWPLDCTLLTNAPAANPAPRLAAVA"
     gene            complement(2714..3718)
                     /locus_tag="P5558_07760"
     CDS             complement(2714..3718)
                     /locus_tag="P5558_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRY24241.1"
                     /translation="MRIALLLLALPLALSLVALAQQPIPSTRRVVLVDSLPAIIAERV
                     VPALEAELTSLLAQEGQRVLLSTLRGSLGLELASQQLASSDLVISLGRVAAGSAARAL
                     AGTATPHLFAFVPPSTAARLVSSDGSPARARATGIAGRLSRAVALAMLRQLLAGREGV
                     PQRVGVVHLAELHATDDAARLLARADASLELVPIRVALPPDATTAAIVEAVREAIADA
                     SEGRGFAGFWLTIEPPVDVPALVRAINAGTEGQVLYAAGLEAVAAGALMSLVPEPESM
                     ARDVAAMAKRLLDGREPGELPVQAPSQVDIALNLKTADLLGIVPSPELLELTRGRLYR
                     "
     gene            complement(3754..4563)
                     /locus_tag="P5558_07765"
     CDS             complement(3754..4563)
                     /locus_tag="P5558_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY24242.1"
                     /translation="MADRLALALPPWAEGVLVIGDVHGQARLFQAAVDLATAERRYVL
                     SLGDLVDRGPDNDGCIRLMLDLLGRKTGTFIRGNHDDKLHRTLKGNPTSVDSDLAVTL
                     EQLDAAPDSKALKRGFCSAFRAAPFVATLGNSVMVHGAFARAMLASRTFHPRLKALAL
                     YGETASGGGGSKPVRTYRWAEKVRAGMTVVIGHHPISDRVVLVRANKLGGKVVHLDCG
                     AGKGRGLGVLRLASDGRIENASRIAYAGHGPELEPAPLVMIEELDGKEEIG"
     gene            complement(4556..4777)
                     /locus_tag="P5558_07770"
     CDS             complement(4556..4777)
                     /locus_tag="P5558_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRY24243.1"
                     /translation="MVELDTRGLNCPLPVLKAQKALRSLAAGERLRVLATDPKAPADF
                     DQLCEVSGHRLVESVELDGHLVLVIEKRG"
     gene            4906..5409
                     /gene="mobB"
                     /locus_tag="P5558_07775"
     CDS             4906..5409
                     /gene="mobB"
                     /locus_tag="P5558_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRY24244.1"
                     /translation="MRVISVVGWKNNGKTTLVVRLIEHLTERGLRVSTVKHAHHSVDL
                     DQPGKDTWRHRQAGAGEVILATGRRWILMHELRDEPEPPLMPLLAKLAPADLVIVEGF
                     KGTSLPKIEVHRGERGTGLIARDDPDVVAVAADIPIDDLPVPVLHLDDIPAIARLALD
                     RAREIET"
     gene            5472..6656
                     /locus_tag="P5558_07780"
     CDS             5472..6656
                     /locus_tag="P5558_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRY24245.1"
                     /translation="MERLRQRVGPIVGTETVPLAEAAGRVLAAPLVSPRAVPAFDNAA
                     VDGFAFAATSQASTGPSRLTLVSDRAAAGHPATAAVPPGHAMRVLTGAMMPAGTDTVA
                     MQEEVVEDGTSLTLARPMRPSSNRRRAGEDIAAGATLFATGLLLRPHHVAVAAEIGLA
                     RLPVFERLRLALLSSGDELREPGGSLPPGALFDANRYLLRSLLRNLPIEVDDLGILAD
                     EPDAVAAAMLAAAGTHHVILSSGGASRGDEDHVVRTVERHGRLHFWQIAMKPGRPLAF
                     GHLGEALTIGLPGNPVAAAVCFMRLARPVLARLAGADWLEPRAFTLPAAFELTKKPGR
                     TELLRARLVGSTVEIIRRQGSGILTSLTDADGLVEVAESTTRIVPGDSVKFFSFNELG
                     AV"
     gene            6653..7792
                     /locus_tag="P5558_07785"
     CDS             6653..7792
                     /locus_tag="P5558_07785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY24246.1"
                     /translation="MSDSETDGDTFDIIVIGAGIAGAGIAWNLAADARVAIVEREDQP
                     GYHTTGRSAAVYLKGYGTEVIRDLTVLSEAFLAGPPADFSEVPLIHPRGALTLVRPDQ
                     MDRLDRYLARQRRHLPHVERLDPKAILAMVPALDPAYAAAAVYDGDARDLDVDAIFQG
                     FLRGFRQRGGKLLTSAEVRTVDHGNGLWHVGTARGSLHAPVVVNAAGAWADELAALAG
                     LGPLGMVPKRRTALIVRPPDGLDVSGWPCVDDIDEQFYFRPEAGKLLCSPADTTPSEP
                     CDAQPEELDIAIAIDRIERALPLGIRRVERSWAGLRTFVADGSPVVGFDPRAEGFFWL
                     AGQGGYGIQTSPALSQLAARLATRLELNGKELEIAAALGPDRFLG"
     gene            complement(7789..8019)
                     /locus_tag="P5558_07790"
     CDS             complement(7789..8019)
                     /locus_tag="P5558_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HRY24247.1"
                     /translation="MTRQLLHLVFGGELKDPQGTEFVDTEKLDIVGIYPSYAAAERAW
                     RAKAQATVDDAHTRYFVVHLHRFLDPDEPTKS"
     gene            8109..9308
                     /locus_tag="P5558_07795"
     CDS             8109..9308
                     /locus_tag="P5558_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY24248.1"
                     /translation="MTAPLPLTLLLCAVQMLAMTGSLTFQALIPVFIREWTLTNTEAA
                     WIGGASYVGYAIAAPILVAATDRIDARSIVLSACLVGGIGGIGFALFAEGFWSAAGWR
                     VLTGVAIAGSYMPGLKALTDRLPEGRPGGRFPALYAATFSLASATSLFLAGLLTSTFD
                     WHAAFALTGLTAFTGAALVSLLPKRSPRPASTGGHALTRVGAVLRDRQTMRFILAYAG
                     HSWEMFAFRTWIVAFLTFVAARSGDGNLVLVGLAATALVVAGLPAGLLGNELAERSGR
                     RRAVATLMLAAAAMAALMAFATDQGFVLVACLALVYGALVMADNSAILVGTIQSTPPE
                     RRGAAIAVQTFLAATMALLSPLAIGGVLDAFGADATAWGLAFGVMGLGAATGALAMLW
                     PGERGTA"
     gene            complement(9305..10318)
                     /locus_tag="P5558_07800"
     CDS             complement(9305..10318)
                     /locus_tag="P5558_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24249.1"
                     /translation="MAVIDLAPGVTRGLVVPAALAPVDAMLADRLGTEAATVVHGFAG
                     GILVAVAACLTGERPGAIDRLPDGGGRIVLQKAHCIDLGCRLTQILAAAGAVPVEIGA
                     VNGTALYQLEEALAEGAAGALFVVGAGVDGGALLELPSFLFAAHSRQVPVVVVAPDRV
                     DWTGLLDAGADLVVLDATTAFGGPGAGIVAGRTALVRAARLQRLGIGRLVQPTPALLA
                     ELAGALAVDRAALVEDRRDRVAARFSGIAGIAVEPGDGGLVLVVDAALAGFSARDLAF
                     ALQGGEPAVLLDDRKASTGRLVLDLARPDDATLEQALLAIGACIEVDVAPAPWPGRRL
                     DGS"
     gene            10414..11574
                     /locus_tag="P5558_07805"
     CDS             10414..11574
                     /locus_tag="P5558_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY24250.1"
                     /translation="MLLHSLRALSATPTSRAIALVFIANGFVLANWFTRIPATKEALG
                     LGDGALGLALMGMPLGSALILPFSGRLVGRFGAGTLAAITGLATATAAALPAFAVDIW
                     SLAFAMFLLGFANGAMDVSMNAKGAAIERALGFNIMSALHGMYGVGVLLGSTAGSLFA
                     GLAIPVAVHLPLTSALAIAALLAIRAALADRPAEASGPGEKMFAFPHGPLWPLACIAV
                     AAFLAEGAAADWSAVYVRTVLDAPPGIAALALAGFSLGLTLTRFIADSLGERFGELTL
                     LRGGSALAGIGMTAGLLQSGTAIAILGFVALGAGLAAVVPIAFRRASNTPGIPPGTGV
                     AAVATVGYTGFLAGPPLIGLLAEAIGLRAALFIIPILAIGIATLGVASIRRS"
     gene            complement(11571..12044)
                     /locus_tag="P5558_07810"
     CDS             complement(11571..12044)
                     /locus_tag="P5558_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRY24251.1"
                     /translation="MRLKILLAALLLPAASAVADPLPDAIDGWAVHRSAYDYPTLVDR
                     LDAAIGESSLNKLSAASATVGAKNLGLEIPGNMVVHAFAPQFAVRMLEASIAAGYEAP
                     LRFYLTEDADGTATLSYKTASFVFAPYADGGEELKLLAAELEEIQAAIAAAAVGS"
     gene            complement(12140..13747)
                     /locus_tag="P5558_07815"
     CDS             complement(12140..13747)
                     /locus_tag="P5558_07815"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HRY24252.1"
                     /translation="MSESFDYVIVGAGSAGCVLANRLSADPTTSVLLLEAGPEDKNPW
                     IHVPIGYYRTIFNPAVAGNFVTEPEPELGGRSINWPRGRVLGGSSSINGLAYVRGQAE
                     DYDHWRQLGNPGWAWDDVLPAFKKLEDSELGDPALRGKGGPLRVSGPIYRSPLMDAFI
                     EAAEATGIPRNPDYNGTRQDGVAYFQLTIKGGKRCSAAVAYLKDIRGRSNLKIATGAL
                     AAGVRMQDGRAVGVDYLQDGAQRTAEARGEVVLAAGAIQSPQLLLLSGIGPDGALKAH
                     GIARQHELPGVGQNLQDHFQARAVYRAPFPATLNDVGNSLWRRGLAGLQWGLFKTGPL
                     TMGAGCVALFWKTREELATPDVQFHVIPFSADKPGEPLHPFSGYTVSVCQLRPESRGE
                     LTLRSADPTDPPVIHANYLATETDRQTMVDGMKLIRRVMGQAAMRPYIAEEVVPGASC
                     ADDAALLGFVRSTGGTIFHPSGTCKMGPADDRMAVVDARLRVHGVAGLRVADASIMPT
                     VISGNTNAPTIMIGEKAAEMILADAKA"
     gene            complement(13744..14331)
                     /locus_tag="P5558_07820"
     CDS             complement(13744..14331)
                     /locus_tag="P5558_07820"
                     /inference="COORDINATES: protein motif:HMM:NF016180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HRY24253.1"
                     /translation="MVELRERPVILRPATTAEGSRCRVAERLGLCLTQVIARPGQERE
                     LARILGQKLPERPGTAMTIGDGTVMALRPRDWLLVRHDPVGRRGGLAVDLRRRLDGVA
                     LVIDQSHGRVVLRLEGAASRSVLQKGIDIDLHPDQFGPGAAAQTGIAGMAVLVHAVAD
                     EAFDLYGNRSFAESLLDWLLEAGREWGIVLEASGT"
     gene            complement(14324..17287)
                     /locus_tag="P5558_07825"
     CDS             complement(14324..17287)
                     /locus_tag="P5558_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HRY24254.1"
                     /translation="MSPDAFRLRTGGRIDRTSRLAFTFDGRRYKGLAGDTLASALLAN
                     GVKLFGRSFKHHRRRGIMGFGVEEPNALVTIGNGAAAEPNTRATMVELAEGLEATSQN
                     RWPTLSLDLQAVNGLAGRFLGAGFYYKTFKWPPAAWTGLYEKVIRHAAGLGRASHAAD
                     PDRYDKSFAFCDVLVVGGGPAGLMAALVAGRAGARVTLADEMPALGGRLRHEDRRIDG
                     RGAMLWLGEIERELAALANVTLLRRTTVFGAYDHGTFGAVEQLASDDGRLPRQRFWQI
                     SAGAAVLATGAIERSIAFAGNDLPGVMLASAAQGYLFEHAVVAGWRPVVFTTNSSGYA
                     TALDLVRAGIAVQAVIDNGPVVDSATARALHEAGVPLFRHAVVERAVGGRAVTGVVMR
                     DELGGRRELECDQLLVSGGWNPTLHLAAHRGGRPRWDDQLATFLPPPADGELLPVGAA
                     AGRFALEDCLADGAMAAVDLCRRLGHAARTPTLPGTDEAEAYRITPFFAVEGTETTAF
                     VDLQNDVTVADLRQAAAEGYRSIEHAKRYTTLGMATDQGKTANVTGLAILAEALARPV
                     AEVGTTGFRPPYTPVAIGALAHHHRGPHFAPVRRTPLHAAQVVLGAVFFETGLWLRAR
                     YVPRPGEDMAAASRREVQAVRAGVGVVDVSPLGKIELAGSDARAFLERLYCNGMATLR
                     PGRARYGLMLREDGMVLDDGTIWCFGSGHFMVTTTTANAAAVLSHMELHHQHFWPELD
                     VQFASVSEQWAGLAVAGPRSREVLQRVVDEPALDDASFPFMAVAEGRIGGVVCRIARI
                     SFSGERAFEVFVPAGHAEALLDCLLEAGATDGITLYGLEAMGTMRIEKGHVAGAELDG
                     RTTPRDLGLERMQIRKKPHIGAALRDRPGLTDPARPMLVGLVPVDRSERLRAGALLVP
                     EGAEVVQANDLGRVTSIAWSPGLGHPIALGFLAHGTERLGQRVDAVFPLYRERVTVEV
                     RQSCFVDPEGERLRG"
     gene            complement(17284..17562)
                     /locus_tag="P5558_07830"
     CDS             complement(17284..17562)
                     /locus_tag="P5558_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HRY24255.1"
                     /translation="MRITCPHCGSRGLTEFSQQGDASPLRPDDGAGEAAWVDFVHFRD
                     NPRGEHRELWQHVLGCRTWLVVTRDTATHAILRVEPAADVRGRPRGRT"
     gene            complement(17583..18833)
                     /locus_tag="P5558_07835"
     CDS             complement(17583..18833)
                     /locus_tag="P5558_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HRY24256.1"
                     /translation="MRYSVWSLIRQGLVGNRGWPEAWRDAAPKDRYDVIVIGGGGHGL
                     ATAYYLAALHGITKVAVLEKGWIGGGNTGRNTTIVRSNYLLPGNEPFYEHSLRLWEGL
                     SAELDFNVMFSQRGVLNLCHSDAQRDAFARRGNAMLMMGADAELLDREAVRAMVPGLD
                     FANARFPITGGLLQRRAGTARHDAVVWGYARAASALGVDIVQNCEVTGMVRDGDRISA
                     VETTRGTIRVDKVGLAVAGNSSRLAAMAGLRLPIESHVLQAFVSEGLKPWLDTVVTFG
                     AGHFYVSQSDKGGLVFGGDIDGYNSYAQRGNLPTVEHVAAGAVALFPALGRVRILRSW
                     GGLVDMTMDGSPIIDRLPLANLYLNGGWCYGGFKATPASGWCFAYTLARDEPHELNAA
                     YRLDRFATGHLIDERGAGPQPGWH"
     gene            complement(18951..19667)
                     /locus_tag="P5558_07840"
     CDS             complement(18951..19667)
                     /locus_tag="P5558_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24257.1"
                     /translation="MFVSSLCFRPQIVAAAVLFLLTACSTTRPVVAPGVTEPVARGST
                     VLLLRPDIECLEVAVGGTQARPEWTAKAEASVTRALADFMREHETRLVVYDGSTFPPD
                     RRAGQEAAIAALTAVRLPASGPAPTAPVQVELPAPELLAPLREDFKADYALSVFLRES
                     FQSAGHVAMRYSLGLLLGPIWAPMPGTGQYGYATLVDLRTGEVAWSNTLSTVGEVPTD
                     IRDPEKARDTVDELLEACPV"
     gene            complement(19691..20296)
                     /locus_tag="P5558_07845"
     CDS             complement(19691..20296)
                     /locus_tag="P5558_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24258.1"
                     /translation="MPGRPVSVADDGVLEVATPQTRFSAVIPAALRDEGVLLPDGSYV
                     FLRSASPLHTVSVLVNGTVVDPGRWPDFRSQMAGSTEAVVLADYLTAVEPLERASPGR
                     PTNTLLSFERVGGARPPQAARNLACSERALVALDHEVPGQAGRPFVYHARYYTCVDPH
                     TRAPVELAWSERLPEDPDGTAPGFMAEADAFFDSLRFLEPR"
     gene            20999..>21190
                     /locus_tag="P5558_07850"
     CDS             20999..>21190
                     /locus_tag="P5558_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY24259.1"
                     /translation="MRELTNGLLFPEGPVALPDGSVALVEIARKTVTVVEADGTKRVL
                     AEPGGGPNGLAMGSDGAFYV"
BASE COUNT         3165 a         7807 c         7159 g         3059 t
ORIGIN      
        1 ccaagccgaa atacagcatg gccctgcatt ccttcacacg ctctctcaga gctcgtcgag
       61 gagcccccgc aggcgctcga gcgcgagcct tagccgctcc gtccgaatcg cgaagcagag
      121 ccgcacatgg ctctcgttgc cggccccgaa cgtgtagccg gcggcgatcc cgaccttgca
      181 gcgctgcagc acctcgcgcg cgaaggcgag gctgtcggtc agcccctcga tccgcggaaa
      241 ggcatagaag gcaccttccg gctcgggcag cgtcaccttc gggtggctgc cgatcagctc
      301 catcaccagg tcgcgattga tcctcgtccg tgcgacgaac gagcggacga attcctcgcc
      361 ctgctccagc gccgcgaggc cgcctgcctg ggcgaagacc gtggcgcctg tgttgttgca
      421 ttcggccagc accgccatgg gctcgaccag gtgctggggt gccaccatcc agccgagccg
      481 ccagccggtc atcgcccagg ccttggagaa gccgttgagc acgaagaccg gctcctcggg
      541 cccggcgagc tcgaggaagg agggggccgg atcgcggccg tgaaagacat tgcgatggta
      601 gacctcgtcg gcgatcagcc cgaggccgtt gtggcggcag aggtcgagca gggcgctgcc
      661 ttcctcggcg gacatgatcc agccggtggg gttcgacggc gtgttgacat agaccgcctt
      721 cgttcgaggc gtgatcgccc gcgccacctc gcccaggtcg agctcccagc gccgctcccc
      781 ggccctgagc cggacatcga ccgaccgcgc ccccatcagc cgcgccgcaa cgcggatgtt
      841 cggccagtag gggccgagca ggacgatctc gtcgtcccgg tcgagcaggc attccgcggc
      901 gacgaccacc gaaagcatgg tcgagcccgc gaccgtgatc cggtcggggt gcaggtcgat
      961 gccatagagc cggtcgagat aggtcttgag cccggcacgc agcgccggcg tgccgcgcga
     1021 gtaggagtag aacgtctggc cggaatcgag cgctgccttc gccgcgtcgc gaatgaaggc
     1081 cggcgtgacg atgtcgctct cgccgaacca gagcgggatc acctccggat cgcccagggc
     1141 acccagcgcc accttgctga tgccggatag ctcgaggtcc tcgatctctt tgcgaatggc
     1201 gcccatggag ctcccgatca ctgcgcgaca gcgggtctct tagcgccgtc aggcgaccgc
     1261 ggccagcctc ggggcggggt tggcggcagg cgcgttggtc aggagtgtgc agtcgagcgg
     1321 ccagtcgaag acgacggtcg tgccgcgggt cgtgccgtcg ctctcgacgc gcatcgtgcc
     1381 gccgaggcgc tggacgatcc ggcgcaccag cgccaggccg atgccgctgc cgccgctgct
     1441 ttggccatcg cgaatccgga agatgtcgaa gatgcgatcg tgctcggccg gggaaatgcc
     1501 cgggccatcg tcggtgacgc gaaagatggc gcggcggtcg ccccggcaga cgtcgacctt
     1561 cagctgcacg gcaccacggt cgtggtgctt gatggcattg tccatgaggt tgcgcacgac
     1621 cagcgccaag ggtgcttcgg gcgtgatcag cgtggcgacc ggcgcgtcga tctcgagcgt
     1681 cacgggcttg gcgggtgcga ggagcgccag ctgcgcctcg atcagctcgc gcaccgcgca
     1741 ctgatcggcc ttgccggcga cctggctggc ccgggcataa cgcaggagat ccagcagcat
     1801 cgcatccatg cggccggccg aatcccgcag ccggtcgagg tgatccaggg ccgcctgcgg
     1861 cagggggccg gcatcggcct cgagccagcc ggcgatgttg acgatcgccc gcagcggtgc
     1921 ccggagatcg tggctgaccg ccgccgcgaa atgatcgagc tcggcgttcg agcgctccag
     1981 ctcggcggtg tgcgtcgcga tcgcggtcgc catggcctcc atctcggcgg cgagctcgct
     2041 gagctcgtcc ttgccggcga gctgcaggtc gagcggccgg cggcgccgcg ccagcgcccg
     2101 ggcgttctcc tccagcagac ggatcggccg cacgaccagt cgatccagcc cgacgaagag
     2161 cgagaccgtc atcaggaacc cggtgagcag cagggtcgat gtcaccgcca ggttgacccg
     2221 gcggatggcg aggttccggg catcggctgg atagccaccc acgagcgtcc agtcccaggg
     2281 ctcgaagtgg cgtgcagcga ggagatagga ggttccgcca atcgcctgca cgaaggccgc
     2341 ggcggacgat tcgggcagcg ggctcgcctc gtcgagcacg agcccggccg gcagcaggac
     2401 atcgccctgg ccatccagca cgaagagctc cgtccccgac cgactggcca gctccgccag
     2461 ctcggccagc gccgccgtct cgtagcgctc ccgataggcg ggcacgttct ccagggcata
     2521 ggtctcgagc acccggtggc ggtcctgcac gatcctgagc gccgcattgt cgagcagatg
     2581 cgtcaggagc tcttcgtgct ggccggccca ttgccgaccc tgctcatagc tgctgaccag
     2641 ccattgcagg gtcaagccgg cgagcacgac cggcagcatg acgacgagaa gcttggttcg
     2701 caggcgcatg atctcaccga taaagccgac cgcgcgtgag ctcgagaagc tcgggcgacg
     2761 gcacgatgcc gagcaggtcg gcagtcttga ggttgagcgc gatatcgacc tggctcggcg
     2821 cctggaccgg cagctcgccc ggctcccggc cgtcgagcag gcgcttggcc atggctgcca
     2881 cgtcgcgggc catgctctcc ggctccggca cgagcgacat cagggcaccc gccgccaccg
     2941 cctccaggcc cgctgcgtag agcacctgcc cttcggttcc ggcattgatg gcgcgaacca
     3001 gcgccggcac gtcgaccggc ggctcgatcg tcagccagaa gccggcgaac ccccggccct
     3061 ccgatgcgtc ggcgatcgcc tcgcgcaccg cctcgacgat cgcggcggtc gtggcgtcgg
     3121 gcggcagcgc cacgcggatc ggcaccagct ccagcgaggc atcggcgcgc gccagcagcc
     3181 gcgctgcatc gtccgtggcg tgcagctcgg cgaggtgaac gacaccgaca cgctggggca
     3241 cgccctcccg gccggcaagg agctggcgca gcatggcaag cgccacggcg cgcgacagcc
     3301 gcccggcgat ccccgtggct cgggcgcgag ccgggctgcc atcgctcgag accagccggg
     3361 ccgccgtgct cggcggcacg aacgcgaaga gatgtggcgt tgccgtccct gccagcgccc
     3421 gggcggcgct gccggcagcg acacgcccga ggctgatgac gaggtcggac gatgcgagct
     3481 gctgcgacgc cagctcgagg ccgagcgacc cccgcagcgt cgacaacagc actctttggc
     3541 cttcttgggc gagcaggctg gtcagctcgg cttcgagcgc cgggacgacg cgctcggcaa
     3601 tgattgccgg aagactgtcg accagcacga cacgacgcgt cgaggggatg ggttgctggg
     3661 ccagggcgac gagcgacaga gccaggggaa gagcaagcaa aaggagggcg atacgcacga
     3721 tggacccgct ggccagtcga tgatttagga ttattagcct atttcttcct ttccatcaag
     3781 ttcctcgatc atcacgagtg gcgccggctc gagctctggc ccgtggccgg cgtaggcgat
     3841 ccgcgacgca ttctcgatcc ggccgtcgct ggccagccgc aacaccccga gaccccgccc
     3901 ctttccggca ccgcagtcga gatggacgac cttgcccccg agcttgttcg cccgaaccag
     3961 gaccacccgg tcgctgatcg gatgatggcc gatgacgacc gtcatgccgg cccgcacctt
     4021 ctcggcccag cgataggtac ggaccggctt gctgccgccc ccgcccgacg ccgtctcgcc
     4081 gtagagcgcc agcgccttca gccgaggatg gaaggttcga ctcgccagca tggcgcgagc
     4141 gaaggcgcca tgcaccatga ccgagttgcc gagggtcgcg acgaagggtg ctgcccgaaa
     4201 ggcgctgcag aagccacgct tgagcgcttt cgaatccggt gccgcgtcga gctgctccag
     4261 ggtcacggca agatcgctgt cgacgctcgt ggggttgccc ttcagcgtgc gatggagctt
     4321 gtcgtcgtga ttgccgcgaa tgaaggtgcc ggtcttgcgg cccagcagat ccagcatgag
     4381 ccggatgcag ccgtcgttgt cggggccgcg atcgaccagg tcgccgagcg acaggacgta
     4441 gcgccgctcc gccgtcgcca gatcgaccgc cgcctggaac agccgggcct ggccatgcac
     4501 gtcgccgatg accaggacac cttcggccca gggcggcagg gccagggcca gtcgatcagc
     4561 cacgcttctc gatcacgagg accagatgac catccagctc gacgctctcg acgagccgat
     4621 gcccggagac ctcgcagagc tggtcgaagt cggccggcgc cttggggtcg gtcgccagca
     4681 cccggagccg ctcgccggcg gcgagggacc ggagcgcctt ctgcgccttg agcaccggca
     4741 gcggacaatt caatccgcgc gtatccagct ccaccatacg tgctttgtcc ggacctgcca
     4801 ccgcccccga tcgcgctgcg ttccccgtgt ggccgaacca ccatccgcat ggtaagcgga
     4861 gtgcggcggg atgagccgcc acagcaggtg tgcagcccaa ttaccatgcg cgtcataagt
     4921 gttgtcggtt ggaagaacaa tggcaagacg accctggtcg tccgcctgat cgagcatctg
     4981 accgagcgcg ggctgcgggt ttcgaccgtc aagcacgccc atcacagcgt cgatctggat
     5041 cagcccggca aggacacgtg gcgccaccgg caggccgggg ccggcgaggt gatcctcgcc
     5101 acgggccgtc gctggatcct gatgcacgaa ttgcgggacg agccggagcc cccgctcatg
     5161 ccgctcctcg cgaagctggc accggccgac ctggtgatcg tcgagggctt caaggggacc
     5221 tcgctgccca agatcgaggt ccacaggggc gagcgcggca ccgggctcat cgcccgcgac
     5281 gacccggatg tcgtcgccgt cgccgccgac atcccgatcg acgatctgcc ggtgccggtc
     5341 ctccacctcg acgacatccc ggccattgcc cgcttggcgc tcgaccgtgc ccgggagatc
     5401 gagacctgac tgaggcacca cccttcgact gcttcgattc cggccgcggg catatcggcc
     5461 tcgcggcggc gctggaacgg ctgcgccagc gggtcggccc catcgtcggc acggagaccg
     5521 tgccgttggc cgaggcggcc ggccgcgtgc tcgcggcccc gctggtcagc ccgcgcgccg
     5581 tgccggcctt cgacaacgcg gcggtcgacg gcttcgcctt cgccgccacc agccaggcat
     5641 cgacgggacc gtcgcgcctc actctcgttt ccgaccgcgc cgcggccggt catccggcca
     5701 cggccgccgt gccgcccggc catgccatgc gcgtgctgac cggtgccatg atgccggcgg
     5761 ggaccgacac ggtcgccatg caggaggagg tggtcgagga cgggaccagc ctgacactcg
     5821 ccaggccgat gcgcccctcc agcaaccgcc gccgcgccgg cgaggatatc gcggcaggtg
     5881 cgacgctctt cgccacgggc ctgctgctcc gcccccacca tgtcgccgtc gccgccgaga
     5941 tcgggctggc acgcctgccg gtcttcgagc ggctccggct ggcgctgctg tcgagcggcg
     6001 acgagctgcg cgagccgggc ggcagcctgc cgccgggagc actcttcgac gccaaccgct
     6061 atctcctccg aagcctgttg cggaacctgc cgatcgaggt cgacgacctc ggcattctcg
     6121 cggacgagcc cgatgcggtg gcggcggcga tgctggcggc cgccggcacg caccacgtca
     6181 tcctttcctc gggcggggct tcccggggtg acgaggacca tgtcgtccgt acggtcgagc
     6241 ggcatggccg cctgcatttc tggcagatcg cgatgaagcc gggccgcccg ctcgccttcg
     6301 gccatctcgg cgaggcgctg accatcggcc tgcccggcaa tccggtggcc gccgcggtct
     6361 gcttcatgcg cctggcacgc cccgtcctcg cccgcctggc cggcgccgac tggctcgagc
     6421 ccagggcctt caccctgccc gccgccttcg agctgaccaa gaagcccggc cgcacggagc
     6481 tgctgcgcgc ccgtctggtg gggagcacgg tggagatcat ccgtcgccag ggctccggca
     6541 tcctgacctc gctgaccgac gccgacggct tggtcgaggt ggccgagagc acgaccagaa
     6601 tcgtcccggg cgattcagtg aaatttttca gcttcaacga attgggggca gtttgagcga
     6661 cagcgagacc gacggggaca cgttcgacat catcgtgatc ggggccggca tcgccggtgc
     6721 cgggatcgcc tggaatctcg cagccgatgc acgggtcgcc atcgtcgagc gcgaggatca
     6781 gcccggctac catacgaccg gccgctcggc cgccgtctat ctcaagggct acggcaccga
     6841 ggtcatccgc gacctgaccg tgctgagcga ggccttcctc gccggcccgc cggccgattt
     6901 cagcgaggtg cccctgatcc acccgcgcgg cgcgctgacc ctcgtccgcc ccgaccagat
     6961 ggacaggctc gaccgctacc tggcacgcca gcgccgccac ctcccccacg tcgagcggct
     7021 ggaccccaag gccattctcg ccatggtccc ggccctcgat ccggcctacg cggccgccgc
     7081 ggtctatgac ggcgatgccc gggatctcga tgtcgacgcg atcttccagg gcttcctgcg
     7141 cggctttcgc cagcgcggcg gcaagctgct cacctcggcc gaagtccgga ccgtggacca
     7201 tggcaacggc ctttggcacg tcggcacggc acgcggcagc ctgcatgcac ccgtcgtcgt
     7261 caatgcggcg ggtgcctggg ccgacgagct ggcggcgctg gccggcctgg gtccgctcgg
     7321 catggtgccg aagcgccgca ccgccctcat cgtgcgcccg ccggacgggc tggacgtcag
     7381 cggctggccg tgcgtcgacg acatcgacga gcagttctac ttccggccgg aggccggcaa
     7441 gctgctctgc tcgccggcgg acacgacgcc atccgagccc tgcgatgccc agcccgagga
     7501 gctcgacatc gcgattgcca tcgaccgcat cgagcgtgcc ctgccgctcg gcattcgacg
     7561 cgtcgagcgg agctgggcgg gcctgcgcac cttcgtcgcg gacggctcgc cggtcgtcgg
     7621 cttcgatccg cgcgccgaag gcttcttctg gctcgccggc cagggcggct acggcatcca
     7681 gacctcgccg gccctgtcgc aactggccgc ccggctcgcc acgcggctgg agctcaacgg
     7741 gaaagaactg gagatcgccg ccgccctcgg gcccgaccgg ttcctcggct agctcttcgt
     7801 cggctcgtcc ggatccagga accggtgcag gtggaccacg aagtagcgcg tgtgcgcgtc
     7861 gtcgaccgtc gcctgcgcct tggcccgcca cgcccgctcg gcggccgcgt agctcggata
     7921 gataccgacg atatcgagct tttcggtatc gacgaactcg gtgccctgcg ggtccttgag
     7981 ctcgccgccg aagacgaggt gcagcaactg cctggtcatg cgcgtcccct tgtgcgaccg
     8041 ggatggccgg ccattcgcag cggatcgact ccgggcaaac ctatgccacc cggtggacac
     8101 acgatgcaat gaccgcgcca ctgccactga cgcttctgct ctgcgccgtg cagatgctcg
     8161 ccatgaccgg ctcgctgacg ttccaggcgc tgatcccggt cttcatccgg gaatggacgt
     8221 tgaccaacac cgaggcggcc tggatcggcg gcgcctcgta tgtgggctat gcgatcgccg
     8281 ccccgatcct ggtcgccgcc accgaccgca tcgatgcccg cagcatcgtg ctctctgcct
     8341 gcctcgtcgg cggcatcggc ggcatcggct tcgccctctt cgccgaaggc ttctggtcgg
     8401 ccgccggctg gcgcgtgctc accggcgtcg ccatcgccgg cagctacatg ccgggcctga
     8461 aggcgctcac cgaccggctg cccgagggcc ggcccggcgg ccgcttccct gccctctacg
     8521 ccgccacctt ctcgctcgcc tcggccacct cgctcttcct cgccggcctg ctcaccagca
     8581 ccttcgactg gcatgccgcc ttcgccctga ccggcctcac cgcgttcacc ggagcggctc
     8641 tcgtctccct tctgccgaag cgttcgccca ggcctgcctc caccggcggc cacgccctga
     8701 cccgcgtcgg cgccgtcctg cgcgatcgcc agaccatgcg gttcatcctg gcttatgccg
     8761 gccattcctg ggaaatgttc gccttccgca cctggatcgt ggccttcctg accttcgtgg
     8821 cggcacgctc cggcgacggc aatctcgtgc tggtcggcct tgccgccacc gccctggtcg
     8881 tcgccggcct gccggctggg ctgctcggca acgagctcgc cgaacgttcc ggccgccgtc
     8941 gcgccgtggc caccctgatg ctggccgccg ccgccatggc ggcgctgatg gcgttcgcca
     9001 ccgaccaggg ctttgtcctc gtcgcctgcc tcgcgctggt ctatggcgcc ctcgtgatgg
     9061 ccgacaactc ggcgatcctg gtcggcacca tccagagcac gccacccgaa cgtcgcggtg
     9121 cggccatcgc cgtgcagacc ttcctcgccg ccacgatggc cctcctgagc cccctcgcca
     9181 taggcggcgt gctcgacgca ttcggcgccg atgccacggc ctggggcctc gccttcggcg
     9241 tcatgggact tggtgcggcc acgggtgcgc tcgccatgct ctggccgggc gagcgcggca
     9301 cagcctagga gccgtccagc ctgcggccgg gccagggcgc cggtgccacg tcgacttcga
     9361 tgcaggcacc gatcgcgagc agcgcctgct cgagcgtggc gtcgtccggt cgggcgagat
     9421 ccagcacgag ccgcccggtc gacgccttgc gatcgtcgag cagcacggca ggctcgcctc
     9481 cctgtagggc aaatgcgaga tcccgagccg agaagccggc gagcgccgcg tcgacgacca
     9541 gcacgagacc gccatcgccc ggctcgacgg cgatcccggc gatgcccgaa aaccgggccg
     9601 ccacgcggtc gcgccggtcc tcgaccagcg ccgcccgatc cacggccagg gcccctgcga
     9661 gctcggctag cagcgccggc gtcggctgca ccagccggcc gatgccgagc cgctgcaggc
     9721 gggccgcccg caccagcgcc gtccgcccgg cgacgatgcc ggccccgggc ccgccgaacg
     9781 cggttgtcgc atcgagcacc acgagatcgg ccccggcatc gaggagaccg gtccagtcca
     9841 ctcgatcggg tgccacgacg accaccggca cctgccggct gtgcgcggcg aagaggaagc
     9901 tcggcagctc gagcagcgct ccgccatcca cgccggcacc gaccacgaaa agcgcgccag
     9961 ccgcgccctc ggccagcgcc tcctcgagct ggtagagcgc cgtgccattc acggccccga
    10021 tctcgaccgg caccgccccg gccgcggcaa ggatctgggt gagccggcaa ccgagatcga
    10081 tgcaatgcgc cttctgcagg acgatccgcc cgccaccatc gggcagacgg tcgatggcgc
    10141 ccgggcgctc gccggtcagg catgccgcca ccgccaccag gatgccaccg gcgaagccgt
    10201 gcacgacggt cgccgcttcc gtccccaggc ggtcggcgag catggcgtcc acgggcgcca
    10261 acgccgccgg cacgacgagc ccccgcgtca cccccggtgc cagatcgatc accgccatcg
    10321 ccacccgccc ttcctgccgg cgttgccaga gccggacccg cagtctaagc ccctcgcttc
    10381 atcgaacaaa gaggcccgga gtcgctaccg accatgttgc tgcacagcct ccgagcgctc
    10441 tcggcgacgc ccacctcgcg cgccatagca ctcgtcttca tcgccaatgg cttcgtgctg
    10501 gccaactggt ttacccggat cccggcaacc aaggaggcgc tcgggctcgg tgacggtgcc
    10561 ctcggcctcg ccctcatggg catgccgctc ggctcggcgc tcatcctgcc attcagcggc
    10621 cggctggtcg gccgcttcgg cgccggcacg ctcgccgcca tcaccggcct cgcgacggcc
    10681 accgccgcgg ccctgccggc cttcgcggtc gatatctgga gtctggcgtt cgccatgttc
    10741 ctgctcggct tcgccaacgg ggcgatggat gtgtcgatga acgccaaggg cgccgcgatc
    10801 gagcgcgccc tcggcttcaa catcatgtcc gccctgcacg gcatgtacgg cgtcggggtg
    10861 ctcctcggca gcacggccgg cagcctcttc gccgggctgg cgatcccggt cgccgtgcat
    10921 ctgccgttga ccagcgcgct cgccatcgcg gcgctgctgg ccatccgcgc cgcactggcc
    10981 gaccggcccg ccgaggcatc ggggccgggc gagaagatgt tcgccttccc gcacggcccc
    11041 ctctggccgc tcgcctgcat cgctgtcgcc gccttcctcg ccgagggtgc ggcggcggac
    11101 tggagtgccg tctatgtccg caccgttctc gacgcaccgc ccggcatcgc cgccctggcc
    11161 ctcgccggct tctcgctcgg cctgaccctc acccgattta ttgcggattc gctcggcgaa
    11221 cggttcggcg agctgacgct gctccgcggc ggctccgccc tggccggcat cggcatgacc
    11281 gcgggcctgc tccagtccgg caccgcgatc gccatcctcg gcttcgtcgc cctcggcgcc
    11341 ggcctcgccg ccgtcgtgcc catcgccttc cgccgcgcct cgaacacgcc gggcatcccc
    11401 cccggcaccg gcgtcgccgc cgtggccacg gtcggctaca cgggcttcct cgccggcccg
    11461 ccgctcatcg gcctgctggc ggaagcaatc gggctcaggg ccgccctctt catcatcccg
    11521 atcctggcca tcggcatcgc cacgctgggc gtcgcctcga tcaggcggag ctaggagccc
    11581 accgcagccg ccgcgatcgc cgcctggatc tcctcgagct cggccgccag cagcttcagc
    11641 tcctcgccac catccgcata gggcgcgaag acgaagctcg ccgtcttgta ggacagggtg
    11701 gccgtgccat ccgcgtcctc ggtcaggtag aagcgcaagg gcgcctcgta gccggcggcg
    11761 atgctcgcct ccagcatgcg cacggcgaac tgaggcgcga aggcgtgcac caccatgttg
    11821 ccgggaatct cgaggccgag gttcttcgca cccaccgtgg cgctcgccgc ggagagcttg
    11881 ttcaaggagc tctccccgat cgccgcatcc agccgatcga ccagggtcgg gtagtcgtag
    11941 gcgctccggt gcaccgccca cccgtcgatc gcatcgggca acgggtcggc cacagccgaa
    12001 gcggccggca ggagaagggc ggcaagcagg atcttcaggc gcatttccat gactcccatg
    12061 gtcgatgatg cgccgttcat gccggggtcg gcatcttcac gccaatgacg tcggttcaca
    12121 ggccggtgac gatcgcccgt caggccttgg cgtccgccaa gatcatctcg gccgccttct
    12181 cgccgatcat gatcgtcggg gcgttggtgt tgccggagat cacggtcggc atgatcgagg
    12241 cgtcggcgac gcgcagcccc gccacgccgt gcacccgcag gcgcgcatcg accaccgcca
    12301 tccggtcgtc agccgggccc atcttgcagg tgccggaagg atggaagatc gttccgccgg
    12361 tcgaccgcac gaagccgagc agcgccgcat cgtccgcgca agacgcaccc gggacgacct
    12421 cctcggcgat atagggccgc atcgccgcct gccccatcac ccggcggatc agcttcatgc
    12481 cgtccaccat ggtctgccgg tcggtctcgg tcgcgagata gttggcgtgg atcaccggcg
    12541 ggtcggtcgg gtcggccgag cgcagcgtca gctcgccgcg gctctcgggc cgaagctggc
    12601 agaccgacac cgtgtagccc gagaaggggt gcagcggctc gccgggcttg tcggcgctga
    12661 acgggatgac gtggaactgc acgtccggcg tcgccagctc ctcgcgcgtc ttccagaaga
    12721 gcgccacgca accggcaccc atggtcagcg gccccgtctt gaacagcccc cattgcagcc
    12781 cggcgaggcc acgccgccag aggctgttgc cgacatcgtt cagggttgcc gggaagggag
    12841 cccggtagac ggcgcgcgcc tggaaatggt cctgcaggtt ctgcccgacg cccggcagct
    12901 cgtgctgccg ggcgatgccg tgcgccttga gcgccccatc cggcccgatg cccgagagca
    12961 gcaggagctg cggcgactgg atggcgccgg cggcgagcac gacctcgccc cttgcctcgg
    13021 cggtccgctg agcgccgtcc tgaaggtagt ccacccccac cgcccggcca tcctgcatcc
    13081 tgacaccggc ggcgagcgcc cctgttgcga tcttcaggtt cgaccggccc ctgatgtcct
    13141 tcagataggc cacggcggca ctgcacctct tgccgccttt gatggtgagc tggaaatagg
    13201 cgacgccgtc ctgccgcgtg ccgttgtagt cgggattgcg cgggatgccc gtcgcttccg
    13261 ccgcctcgat gaaggcatcc atcagcggcg aacggtagat cggtccggaa acccgcagcg
    13321 gcccgccctt gccgcgcagc gccggatcgc cgagctcgct gtcctcgagc ttcttgaagg
    13381 ccggcagcac gtcgtcccag gcccagcccg gattgccgag ctgccgccag tggtcgtagt
    13441 cctcggcctg gccccgaaca taggcgaggc cattgatcga ggacgagccg ccgagcaccc
    13501 gaccgcgcgg ccagttgatg ctgcggccgc cgagctcggg ctccggctcg gtcacgaaat
    13561 tgccggcgac ggccgggttg aagatcgtcc ggtagtagcc gatcggcacg tggatccagg
    13621 ggttcttgtc ctccggcccc gcttccagca gcagcacgct ggtcgtcggg tccgcgctca
    13681 gccgattggc gaggacgcag ccggcggagc cggcgcccac gatcacatag tcgaagctct
    13741 cgctcacgtc cccgatgcct ccagcacgat gccccattca cgccccgcct cgagcagcca
    13801 gtcgagcagg ctctcggcga acgagcgatt gccatacaga tcgaatgcct cgtccgccac
    13861 cgcatggacg agcaccgcca tcccggcaat gccggtttgc gcagcggcac ccggtccgaa
    13921 ctggtcgggg tgcaggtcga tgtcgatgcc cttctgcagg accgaccggc tcgccgcgcc
    13981 ctctagacgc agcaccaccc gcccgtggct ctggtcgatc acgagcgcta cgccatcgag
    14041 ccgccggcga aggtcgaccg caagaccgcc ccgccggccg accggatcat gacggacgag
    14101 gagccaatct cgtggtctca gcgccatgac cgtcccgtcg ccgatcgtca tggccgtgcc
    14161 cggccgctcg ggcagcttct ggccgaggat gcgggccagc tcacgctcct gtccagggcg
    14221 ggcgatcacc tgcgtcaggc agagcccgag ccgctcggca acccgacacc gagagccctc
    14281 ggccgtcgtt gccggtcgga ggatgacagg ccgctcccgc agctcaacca cgcagccgct
    14341 ccccttccgg gtcgacgaag caggactggc gcacctcgac ggtcaccctt tcgcgataga
    14401 gtgggaagac cgcatcgacc cgttgaccga gccgctcggt gccgtgggcc agaaagccca
    14461 gggcgatcgg atgcccgaga cccggactcc aggcaatcga tgtgacacgg cccaggtcgt
    14521 tcgcctgcac cacttcggcc ccctcgggca ccagcagtgc gccggcccgc agccgctcgc
    14581 tgcggtcgac cggcacgagc ccgaccagca ttggccgcgc cgggtcggtc aatcccggcc
    14641 ggtcgcgaag cgccgcgccg atatgcggct tcttgcggat ctgcatccgc tcgagcccga
    14701 gatcgcgggg tgtcgtcctg ccatccagct cggcccctgc cacatgcccc ttctcgatcc
    14761 gcatcgtgcc catggcctcg agcccataga gggtgatgcc gtccgtcgcc ccggcctcga
    14821 gcaggcagtc cagcaacgcc tcggcatggc ccgccggcac gaagacctcg aaagcccgct
    14881 cgcccgagaa gctgatccgg gcaatgcggc agaccacgcc gccgatccgc ccctcggcca
    14941 ccgccatgaa gggaaacgac gcatcgtcga gcgccggctc gtccacgacc ctttgcagca
    15001 cctcgcgcga cctcggcccc gccaccgcca gccccgccca ctgctcgctc accgaggcga
    15061 actggacatc gagctccggc cagaaatgct ggtgatggag ctccatgtgg ctcagcacgg
    15121 cggcggcatt tgcggtcgtc gtcgtcacca tgaaatgccc ggagccgaag caccagatcg
    15181 tgccgtcgtc cagcaccatg ccgtcctcgc gcagcatcag cccgtagcgc gcccggcccg
    15241 gccgcagcgt cgccatgccg ttgcagtaga gccgctcgag aaaggcgcgc gcatctgaac
    15301 ccgcgagctc gatcttgccg agcgggctga cgtcgaccac accgacgccg gcgcgcaccg
    15361 cctgaacctc gcgccgcgaa gccgccgcca tgtcctcgcc cggtcgcggc acataccgcg
    15421 cccgcagcca caaccccgtc tcgaagaaga ccgcccctag caccacctgc gccgcgtgca
    15481 gcggcgtcct gcgcaccggg gcgaaatggg ggccacgatg atgatgcgcc agggctccga
    15541 tcgcgacggg ggtgtagggc ggacgaaacc cggtcgtccc gacctcggcc actggccggg
    15601 ccagcgcctc ggcgaggatg gcgagcccgg tcacgttcgc cgtcttgccc tggtccgtcg
    15661 ccatgccgag cgtggtgtag cgcttggcgt gctcgatgct ccgatagccc tcggcggcgg
    15721 cctgacgcag atcggcgacc gtgacgtcgt tctgcaggtc gacgaaggct gtcgtctccg
    15781 ttccctcaac cgcgaagaac ggcgttatgc ggtaggcctc ggcctcgtcg gtcccgggca
    15841 gggtcggcgt ccgtgccgca tgcccgagcc ggcggcagag atcgaccgcc gccatggccc
    15901 cgtccgccag gcagtcctcg agcgcgaacc ggccggcggc agcgccgacc ggcagcagct
    15961 cgccgtcggc gggtggtggc aggaaggtgg cgagctggtc gtcccagcgc ggccgcccgc
    16021 cccggtgcgc cgccaggtgc agggtcggat tccagccgcc gctgaccagg agctggtcgc
    16081 attcgagctc gcgccggccg cccagctcgt cgcgcatcac gacgcccgtg accgcgcgcc
    16141 cgccgaccgc ccgctcgacc accgcatggc gaaagagcgg caccccggcc tcgtgcagcg
    16201 cccgcgcggt ggccgaatcg acgaccgggc cattgtcgat caccgcctgc accgcgatcc
    16261 cggcccgcac gaggtcgagc gccgtggcgt agcccgagct gttggtggtg aagacgaccg
    16321 gtcgccagcc ggccaccacc gcatgctcga agagatagcc ctgcgccgcc gaggccagca
    16381 tcacgccggg caggtcgttg cccgcaaagg cgatgctccg ctcgatagca cccgtcgcca
    16441 aaaccgccgc cccggccgag atctgccaga accgctggcg tggcagccgc ccgtcatcgc
    16501 tggcgagctg ctccactgcc ccgaacgtgc cgtggtcata ggcgccgaag accgtcgtgc
    16561 gccgcaagag cgtgacattg gccagcgccg ccagctcccg ctcgatctcg ccgagccaca
    16621 gcatggcacc gcgcccgtcg atgcgccggt cctcgtgccg cagccggccg ccgagcgcag
    16681 gcatctcgtc ggcgagggtc accctggccc ccgccctgcc cgccaccagc gccgccatca
    16741 gcccggccgg cccgccgccg accaccagca cgtcgcagaa ggcgaagctc ttgtcgtagc
    16801 gatccgggtc cgccgcatgg ctcgcccggc cgagcccggc ggcatgccgg atcaccttct
    16861 cgtagagacc ggtccaggcg gcgggcggcc acttgaaggt cttgtagtag aagccggcgc
    16921 cgagaaagcg gcccgccagc ccgttcaccg cctggagatc cagcgaaagc gtcggccagc
    16981 ggttctgcga ggtggcttcg agcccctcgg cgagctcgac catcgtggcc cgcgtgttgg
    17041 gctcggccgc tgccccgttc ccgatcgtga cgagcgcatt gggctcctcg acgccgaagc
    17101 ccatgatgcc gcgtcggcga tggtgcttga agctccggcc gaagagcttg acgccattgg
    17161 cgagcagtgc ggaagccagc gtgtcgccgg cgaggccctt gtagcggcgc ccgtcgaagg
    17221 tgaaggcgag ccggctcgtg cggtcgatcc gcccgccggt ccgcaggcga aaggcgtcgg
    17281 ggctcatgtc cgcccccggg gccgcccccg aacatcggcg gccggctcga cgcggaggat
    17341 ggcgtgggtc gccgtgtcgc gggtcaccac cagccaggtc cgacaaccca gcacatgctg
    17401 ccagagctcc ctgtgctcgc cccgcggatt gtcgcgaaaa tggacgaagt cgacccaggc
    17461 tgcctcgccc gcgccatcgt ccggccggag cggcgaggca tcgccttgct ggctgaactc
    17521 tgtgagcccg cgcgagccgc aatggggaca ggtgatgcgc atcgtcgctg cctcgctccc
    17581 gctcaatgcc agcccggctg cgggccggcg ccgcgctcgt cgatcaggtg ccccgtggcg
    17641 aagcggtcga ggcggtaggc ggcattgagc tcgtgcggct cgtcccgggc cagcgtgtag
    17701 gcgaagcacc agcccgaggc cggcgtcgcc ttgaagccgc catagcacca gccgccattg
    17761 aggtagaggt tggccagcgg cagccgatcg atgatcgggc tgccgtccat ggtcatgtcg
    17821 accagcccgc cccaggagcg caggatgcgg acccgcccga gcgccgggaa gagggccacc
    17881 gcgccggccg ccacatgctc gaccgtcggc aggttgccgc gctgcgcgta cgagttgtag
    17941 ccgtcgatgt cgccgccgaa gacgaggccg cccttgtccg actggctgac atagaaatgc
    18001 cccgccccga aggtcacgac cgtgtccagc cagggcttca gcccctcgct gacgaaggcc
    18061 tggagcacgt ggctctcgat cggcagccgc aggcccgcca tggcggcgag cctggagctg
    18121 ttgccggcga cggcgaggcc caccttgtcc accctgatcg tgccgcgggt ggtctcgaca
    18181 gcactgatgc ggtcaccgtc gcgcaccatg ccggtgacct cgcagttctg gacgatgtcg
    18241 acacccagcg ccgaggccgc gcgggcgtac ccccagacca cggcatcgtg ccgggcggtg
    18301 ccggcgcggc gctgcaggag gccgccggtg atgggaaagc gggcgttggc gaagtcgagg
    18361 cccggcacca tggcccggac cgcttcgcga tccagcagct cggcatccgc gcccatcatg
    18421 agcatggcgt tcccgcgccg ggcgaaggcg tcgcgctggg cgtcggaatg gcacagattg
    18481 agcacgccgc gctggctgaa catgacgttg aagtcgagct cggccgagag cccctcccag
    18541 agcctcaggc tgtgctcgta gaagggctcg ttgcccggca gcaggtagtt cgagcggacg
    18601 atcgtggtgt tgcggccggt attgccgccg ccgatccagc ccttctcgag caccgccacc
    18661 ttcgtgatgc cgtgcagggc ggcgaggtag taggccgtgg cgaggccgtg cccgccgccg
    18721 ccgatgacga tcacatcgta gcggtccttc ggcgccgcat cccgccacgc ctccggccag
    18781 ccgcgattgc ccacgagccc ctgccggatc aggctccaga ccgaatagcg caagcgcacc
    18841 tcccacttcc gcccctcggt agcgcagggc ggggcaaggg gaaagggcgt gcgcgagcgt
    18901 cgaccggagc gccggcgtcc aatccggcag gctggctggc cgcaacctgc tcagaccggg
    18961 caggcctcca acagctcgtc gaccgtgtcg cgggccttct cggggtcgcg aatatcggtc
    19021 ggcacctcgc caaccgtcga gagcgtgttc gaccaggcca cctcgccggt ccgcagatcg
    19081 acgagggtgg catagccgta ttggccggtc cccggcatcg gcgcccagat cgggccgagc
    19141 aacagcccga ggctgtagcg catggcgaca tgaccggcgg actggaagct ctccctcagg
    19201 aaaacgctga gcgcataatc ggccttgaag tcctcgcgca gcggcgccag gagctcgggt
    19261 gccggcagct cgacctgcac cggcgcggtc ggcgccggac cgctagccgg caagcggacc
    19321 gcggtcagcg cggcgatggc cgcttcctgc ccggcccggc gatcgggggg gaatgtcgag
    19381 ccgtcgtaga cgacgagccg cgtctcatgc tcgcgcatga agtccgcgag cgcgcgggtg
    19441 acgctggcct cggccttcgc cgtccattcg ggccgcgcct gggtgccgcc gaccgccacc
    19501 tcgaggcatt cgatgtcggg cctgagcaga agcacggtgg agccgcgcgc aacaggctcg
    19561 gtgacccccg gcgccaccac cggacgcgtg gtggagcagg cggtgagaag gaagagcacc
    19621 gcagcggcaa ctatttgagg acggaagcag agcgacgaca cgaacatgag cacaactcca
    19681 gcaatcaagt ttaccggggc tccaggaacc tcaggctgtc gaagaaggcg tccgcctcgg
    19741 ccatgaagcc gggggcggtt ccgtccgggt cctccggcag gcgctctgac caggcgagct
    19801 cgacgggcgc ccgggtatgg ggatcgacgc aagtgtagta ccgcgcatgg tagacgaatg
    19861 gccggcctgc ttggccgggc acctcgtggt ccagggcaac cagcgcccgt tcgctgcatg
    19921 cgaggttgcg tgcggcctgc ggcggtcgcg cgccgccaac ccgctcgaag ctcagcaggg
    19981 tattggtcgg ccgtccgggt gaggcgcgct cgagcggctc cacggccgtc agataatccg
    20041 ccagaacgac cgcctcggtc gatccggcca tctgcgatcg aaagtccggc caccggccgg
    20101 gatcgacaac cgtcccgttg accaggacgg aaaccgtgtg gagggggctc gccgatcgga
    20161 ggaagacgta cgagccatcg ggcagcagca cgccctcgtc gcgcagggcg gcggggatga
    20221 ccgccgaaaa gcgggtctgc ggcgtcgcta cctcgagcac cccgtcatcg gccacgctga
    20281 ccggccggcc cggcatgcag gcggccacga gcaggagacc gccgacaagc cacccgcgcc
    20341 gtgcgcagaa ccagccagct gaccgacttg ttgggttcat tcgaccccct ccggccgcgc
    20401 gacgcgagct cgaagtggca gactatgcgc cgccgcacgg attcgccttg atccagcgca
    20461 aacgcatcct gggcgcgaca gcgggcggcg cgcgacaagg cgtagcggtg atcggggaag
    20521 gccggcaacc ttgcggctct tgacgatgtc gacgccgggt gccgaggccg cccggccttg
    20581 gctccaagct accgcatcgc gccgggtggg gccgatccgg cgaaccaggg gccgccggtg
    20641 gttggaaagc gggcgctggc gaagtccggc ggcggtggtc gacctatgga caaccccctc
    20701 ggaaatgaca gcgggcgccg aggcccggtg cgagcatcga tgtcgcgatc gaggctgcag
    20761 caggcgagac gcacgccgag gagcaggaag ccggcgctcc ccatcgtggc gatcgcctcg
    20821 ctcatcgagg tgacgccctc tcccaagggc atgctcgaag gtcgcccggc agggcgcctc
    20881 gtgccttgcg gcggccgcga cgctggcaag cgcgccgggg cgcgtgcaca atcgcggcca
    20941 ctgatccgat cgggaggaac ggcgatgaac gagaaccgta tcgaacgagg ggttgcccgt
    21001 gcgtgagctg acgaatggcc tgctctttcc cgagggtccg gtggcgctgc cggacggctc
    21061 ggtcgccctg gtcgagatcg cccgaaaaac cgtcacggtg gtcgaagccg atggcacgaa
    21121 gcgcgtgctg gccgagccgg gcggcgggcc gaacgggctc gccatgggat ccgacggggc
    21181 cttctacgtc
//
LOCUS       DAOTFI010000059        21150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_2996_length_21150_cov_7.023133, whole genome shotgun sequence.
ACCESSION   DAOTFI010000059 DAOTFI010000000
VERSION     DAOTFI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21150
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_21150_cov_7.023133"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="P5558_07855"
     CDS             complement(<1..367)
                     /locus_tag="P5558_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRY24260.1"
                     /translation="MAKRLLVLVATGKGAFILESNQGRQDWTVHGPLCDAQPTNHLVA
                     DPETGTIWAGGGDAWRGAAVWRSTDQGATFEKFTDGLAYPEAHDKIKAVWSLAHAHGR
                     LLTGAEPAGLFESRDDGETW"
     gene            complement(393..1103)
                     /locus_tag="P5558_07860"
     CDS             complement(393..1103)
                     /locus_tag="P5558_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY24261.1"
                     /translation="MPDTAVAHPVVSHAEWLEARKALLAEEKALTRAHDRVNEARRAL
                     PWERVEKDYRFEGPDGPLGLAELFGRNSQLVLYHFMFAPGWSEGCKSCSLLADHFDGI
                     EQHLRHHDVSLAAVSMAPLAELLPFKARMGWRFPWVSSAGSAFNFDFQVSATDADKAC
                     GTQPYNYTTLPGVWGEMPGVSVFTKDDEGTVFHTWSTYARGLDILLGVHNFLDLTPKG
                     RNETTAMDWVRHHDRYTD"
     gene            complement(1114..1569)
                     /locus_tag="P5558_07865"
     CDS             complement(1114..1569)
                     /locus_tag="P5558_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRY24262.1"
                     /translation="MATATATEAPIELRITRTLDAPRDRVFAAWTDPAQLARWWGPKS
                     FTLPAMEADIRPGGQWRACIRSPEGEDHWMGGTWQEIDPVERLVFSFAWESPDALLTL
                     ITVTLKPEGDKTRLTFHQTPFRDALSRDSHMGGWTSCLERLDDHLASLS"
     gene            complement(1690..2073)
                     /locus_tag="P5558_07870"
     CDS             complement(1690..2073)
                     /locus_tag="P5558_07870"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY24263.1"
                     /translation="MPWSHGAFHWNELLTWDVERAKKFYADTIGWTFESMPMPDGTYW
                     VARMGEAYAGGMFQLRKPEHEGLPEFWMAYLAVDDVDARVAKAVSAGAELKRPIFDVP
                     GVGRIAILQQPGGAGIGWMTPASQD"
     gene            complement(2103..2543)
                     /locus_tag="P5558_07875"
     CDS             complement(2103..2543)
                     /locus_tag="P5558_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRY24264.1"
                     /translation="MSATDTRPSLTLRRHFNAPRETVFAAWTDPQKLSRWFGPARVSV
                     LEAEVAAFEGGSYRIVMLDDEGGRHEVGGTYREVVPPERLVFTWAWKSMPERQSVVTL
                     RLAEVQGGTLLTLVHEQFADDATRGRHAQGWTGSLDKLDAFLSA"
     gene            complement(2444..2881)
                     /locus_tag="P5558_07880"
     CDS             complement(2444..2881)
                     /locus_tag="P5558_07880"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY24265.1"
                     /translation="MVNFQSPDLDRTFAALADPTRRALLARLGEAESLSVSELARPFA
                     ISLPGVMKHLAVLSDAGLVERRKVGRTVTCRLNAAPMREAIDWLERYQRFWSESLDRL
                     AAFLEEESEAPCPPPTPDRASPSGDTSTPPGKPSSPPGPTRKS"
     gene            complement(2992..3276)
                     /locus_tag="P5558_07885"
     CDS             complement(2992..3276)
                     /locus_tag="P5558_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24266.1"
                     /translation="MSATTTIRISLSEKLAERLDEVARRTERHRDDVVAEAIQAFLDL
                     DAWHAQAIAEAVIEADAGGPFAHHKDVEAYLDALARGERPKPPQTFFLPR"
     gene            complement(3364..4845)
                     /locus_tag="P5558_07890"
     CDS             complement(3364..4845)
                     /locus_tag="P5558_07890"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRY24267.1"
                     /translation="MAVALKDIKNALSDKSLFIERCYIDGQWVAADSGKTFDVTDPAT
                     GERLGSVPDMGVDETRRAIAAAEAALPAWRAKTGKERAAILEKWFDLIMASKEDLGYL
                     MTREQGKPLAEATGEVVYGAAFVEWFGEEAKRVYGDVIPQTVASRRILAIKQPVGVCA
                     AITPWNFPNAMITRKCAPGLAVGCTFVVKPASMTPYSALALAELAERAGMPKGVFNIV
                     TGSARVIGGELTSNKTVRKVSFTGSTDVGKLLLKQCADTVKRVSMELGGHAPFIVFDD
                     ADIDAAVKGAILCKFRNCGQTCVCTNRIYVQAGVYDEFVEKFKKAASELKVGHGTEKG
                     VEVGPLIEQAALDKSSEHVKDAVAKGAVLTLGGKPHELGGLFFEPTVLANCNADMKIA
                     HEETFGPVAPIFKFETEEEVIKLANDSDVGLAGYFYARDIGRVFRVAEAMEVGLIGVN
                     DGVIAVEVAPFGGVKESGLGREGSKYGVDEYLEIKYVSLGGIS"
     gene            complement(4893..6788)
                     /gene="htpG"
                     /locus_tag="P5558_07895"
     CDS             complement(4893..6788)
                     /gene="htpG"
                     /locus_tag="P5558_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRY24268.1"
                     /translation="MSDTVQPFEAEVGRVLDLVINSLYKEREIFLRELVSNASDACDK
                     LRYASLSQPELLGTDTDLKIEIFADPDAALLTIRDNGIGMDRDELVANLGTIARSGTA
                     RFLEAAEQQKNGDVKLIGQFGVGFYAAFMVADRVVVQSRKAGEETSWLWVSDGRQGFT
                     VSEAPETGPRGVTVTLHLKEDAREFLGEWRLREIIRRYSDHIAFPILLQVKPKADDKE
                     AEAEPKAPEKVNEGGALWTRPRGEITDEQYKEFYHHVAHAFDDPFARVHFTAEGQLSY
                     TALLFVPSMRPFDLYDPKRRHGVKLYVKRVFITDDVEALMPRYLRFVAGVLDSEDLQL
                     NVSRETLQHGAIVQKMRKVLVRRVLDELAKKAKEDGYEAFWADFGPVLKEGLFEDHEN
                     RERLLEIARFRSTEGKGWTTLDGYVERMKEGQADIFYITGTSIEALRSSPQLEMARAK
                     GVEVLLFDDPVDEFWLPEVGEYKGKKLHSLTRSDVDLSSIKGADSPETPRADQAGLDK
                     LIARLKLALGEQVKDVRASKRLKESAVCLVADDGGMDLRLGRMLKGHEQFAGGTKPIL
                     EINAEHALVGRLRQMVDDPTATPDLDELAFLLLDQARIIEGEPVPDPGAFSRRMSRYL
                     ARGIEAA"
     gene            7094..9622
                     /locus_tag="P5558_07900"
     CDS             7094..9622
                     /locus_tag="P5558_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRY24269.1"
                     /translation="MVADPGRIAAVLGPTNTGKTHYAVERMLAHGSGMMGFPLRLLAR
                     EIYDRVVQLRGPSAVALLTGEEQIGSDTARYVIATVEAMPIQRRFAFLAVDEIQLCAD
                     RERGHVFTDRLLHARGEEETLFLGSDTIRPLLKQLVPEAEIVTRPRFSILRHVGPGKL
                     NRLPRRSAAVAFTAADVYTLAEVMRRQKGGAAVVLGALSPRTRNAQVAMYQSGEVDHL
                     VATDAIGMGLNMDLVHVAFAQLHKFDGRHDRRLTAAEIGQIAGRAGRHMADGTFGTTN
                     GSGPLDDRIVEAVESHNFLPLKRLRWRNSDLDFASLEALNESLDADPPAPGLVKVRDA
                     LDDRSLGMLARRDDIRARARGPAGVRLLWQVCQIPDFRRTLTDAHLQLLGIVFRHLTD
                     GPAVLPADWVGSMIARLDRMDGDIDTLVGRIAHVRTWTYLSHRAGWFDDPLHWQGVAR
                     AVEDRLSDALHERLTQKFVDRRTAALLKSLRERGELAAEIEPGGEVVVDGHSVGTLEG
                     FVFTLGASGLETEHRLFAGAARRAVARHLDRLAAELVKEPDGAFTIIGGELVWRDAVV
                     ATIRPGPVRLRPTVAVTATDELTTKARLAIERRLEARLRAWLEDRLGPLFALEAASRS
                     EALSAAARGIAYRLVEAFGMLDAAEAAGLVRQLDSADRKAVARFGVRLGVHHIFLPAL
                     LKPAAQEAGALLVALASGTAAWQPTAGRVALRGDELPADGMALAALGFRRIGRLALRV
                     DMVERVAAQLRRLARGEAQFSLPAELVSATGLGRDEFMELLPGLGFRAMAPAADDVPA
                     GAINIRRAKPRRRPSAAHRKERAQAGPSQSPFAVLARLKVAAGA"
     gene            9638..9901
                     /locus_tag="P5558_07905"
     CDS             9638..9901
                     /locus_tag="P5558_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRY24270.1"
                     /translation="MTEGGLRLDKWLFHARFIKQRVLAVELVRRRRIRLNDRLVEKVH
                     STVRPGDVITLVAPSGVAVLKVLALGERRGPAPEARTLYEELS"
     gene            complement(9898..10590)
                     /locus_tag="P5558_07910"
     CDS             complement(9898..10590)
                     /locus_tag="P5558_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRY24271.1"
                     /translation="MPEALDHTVDVAANLAAVRARILEAAAEAGRDPAAVTLIAVGKA
                     QPLERLEAALEAGQRVFGENYVQEAQGRWPALRARYPEVELHLIGALQTNKAKDAVAL
                     FDVIQTVDRPRLAAALEKEMTKQGKALRCFVQVNTGKEPQKAGIMPEETEAFVRLCRE
                     SHGLDVRGLMAIPPADEDIALHTAFLVKLAERSGLEQVSIGMSDDYADAVRFGATHVR
                     VGSAIFGARPPR"
     gene            10670..11857
                     /locus_tag="P5558_07915"
     CDS             10670..11857
                     /locus_tag="P5558_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY24272.1"
                     /translation="MSMGFDQILAPLGGETFLRDYLGQRPVHIEGGAEKFRELMSWSV
                     LNRLLGMSTIWDAERLVMVLDKEEVPPAAYLGRDPATSGRPAADPSRVKQYIKQGATL
                     VLNDIDQLTPELSAFARTMETALGGKVQGNLYQSSKRRQGFKVHYDTHDVFAVHCEGE
                     KVWPVFQGRAEDPIAHPIFKNVPQEAHEKAKGELWKEVRMKPGDLLYLPRGQYHYALA
                     DDGGCIHIAFGVTYPIGIDVMSYLFERMITDPLCRRNLPRGDAGALAAHLGTLGERLG
                     EVLAEPRALADMQAFQAGYRYQRGSYDFPELLDPASLRYTVNGKGVRLIEQQGRFGLV
                     KEGSRQAVGVPGEVSGMVAWVLKRPGFSRAELDEAFPAADKATLDKFIVDMERMLLIA
                     VVA"
     gene            11871..13019
                     /locus_tag="P5558_07920"
     CDS             11871..13019
                     /locus_tag="P5558_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY24273.1"
                     /translation="MVDVFFWVQHLLGIGHLARAATLVRAMRRHGLEVTLASGGLPVP
                     SLDLEGGELLQLAPVQCGTGDFSTLLDAAGEPVDEAFKQRRARALVEAFEVRRPRLLL
                     LEMFPFGRRQMRFELEPLLEAARALVPRPPIVSSVRDILVRKSRPEREAEMLARALTF
                     DAVLAHGDPAFAPFSATFPASARLGDRLIETGYVVDPLPPDDGRSTDGQGEIIVSAGG
                     GRVGERLLETAIEASAFARGGPAWRVLVGWQVPEADFAALARRARPGLVIERARPDFR
                     TLLGRCALSVSQGGYNTVVEVLAAGCRAVVVPYAEDGETEQAERCRRLAERGLLHPLA
                     ARELDPPGLARLVAQVLASPARTGPPPRLDGADVTARLLRRLSRPTGR"
     gene            13245..14312
                     /locus_tag="P5558_07925"
     CDS             13245..14312
                     /locus_tag="P5558_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRY24274.1"
                     /translation="MNLRNALLGTTAMIGAAALVVPSTASAFDVNINGFLNFNAVGGD
                     TSEALGQSASSFDFVTDTEIHVQGVQTDDETGIRYGMTVEFEADPQTSNNNNIDEAWI
                     FIDGSFGGIRLGNEDGIVDNSKLGAYTIAAGTGGIDGQGVVGAVKFAPVNSGDSTKAR
                     YYSPTIAGFSVGVSYTPNGGVEGNQVADTQSVQYTDMFEGGLVYSNSFGSLDLLASAV
                     GIYATGMDSNTGGNNFDRDDYTGYNFGAVVGFAGFEVAGSYWGEQDAPAGNNANNSVN
                     RNGFTAGAAASFGPADLSVTYAKVTNGGGGVGEGDNVVLSASMGLLPGMALQGDVSWF
                     DRDAGGDDDGVTGVARVRVAF"
     gene            14615..15205
                     /locus_tag="P5558_07930"
     CDS             14615..15205
                     /locus_tag="P5558_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24275.1"
                     /translation="MDLVPGGYGVADPDAMQAGQGNEARVGTTGSVAGLTFDFTPRAI
                     GSLINPSMLDPSTEAAGQQPLSLTLGVQNRYAEGLRLGSVGLVEPSTEATHGLALGGA
                     FDINDWQLTGSYGRTNLMGEAADVFAAGIGYGPMSARLVYGHVPRTTGPSGDVLMFST
                     DLAAWSWLTLEGDVAVSDSLAEEPLTVGRVGLRLNF"
     gene            15297..15884
                     /locus_tag="P5558_07935"
     CDS             15297..15884
                     /locus_tag="P5558_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HRY24276.1"
                     /translation="MLDETMEIPKSMTQKSLAARGRWLILLAVLAVTACEGVEAEPPP
                     QTTTGTARDQRDRERFGTITGEGPLISFSTDRARAPNEGVGGGIGVNAFLWEATLDTI
                     NFMPLASADPFGGLIITDWFQPATTPGERVKLHVQIRDAALRADSVTVSVFRQQRDPA
                     GDWLDAPADATTAREIEDHILTRARELRVASAAAG"
     gene            15941..18505
                     /gene="leuS"
                     /locus_tag="P5558_07940"
     CDS             15941..18505
                     /gene="leuS"
                     /locus_tag="P5558_07940"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRY24277.1"
                     /translation="MSRYPFRATEQKWQQVWDERRSFEVGIDRTKPKYYVLEMFPYPS
                     GRIHMGHVRNYTMGDVVARARRAQGYNVLHPMGWDAFGLPAENAAMQKGVHPGVWTYA
                     NIAEMRSQFKQMGLALDWSRELATCDVEYYRHEQAMFLDMLERGLVYRKESWVNWDPV
                     DQTVLANEQVIDGRGWRSGAEIERRKLNQWVFRITAFADELLAALDDLERWPEKVRLM
                     QTNWLGRSEGALVHFPVQGREEPLTVFTTRPDTLWGASFMAIAPDHPLAQELAEADPA
                     LAAFIEECLKLGTSEEAMERAEKKGFDTGLRCRHPLGATPDLPVYVANFVLMGYGTGA
                     IFGCPAHDQRDLEFARKYGRPVTPVVLPEGADPASFAIGAEAYVGDGTIFNSGMLDGL
                     AVAEAKRRIIEHLATEGLGERQVTWRLRDWGVSRQRYWGCPIPVLYTEDGEILPVPRD
                     ALPVRLPDDVDFSAPGNPLDRHPTWKHVTLPDGRRATRETDTFDTFVESSWYFARFCS
                     PRTEDRPFDRAAVDYWLPVDQYIGGVEHAVLHLLYSRFFTRAMSACGHLDLAEPFAGL
                     FTQGMVTHQTYRDPHGRWLQPDEVTRGADGELVTARGARPVSVGRVEKMSKSKRNTVD
                     PAVIIEAYGADTARLFMLSDSPPERDLEWTDQGIDGAWRYLNRLWRLVDERRAELPAA
                     GGAASAPASEAAQELKRLVHRSIAAVTTEIDRLHFNKAVALVRELSNAIEGYEAAGEN
                     DRQVLREALETLVQLIGPMLPHLAEELWERLGHRTILADTPWPVADPAWLAAASVTIA
                     VQVNGKRRAAIDVPVGSDKTSVEAAALALEAVKRAIDGQPVRRVIVVPDRIVNVVV"
     gene            18492..19061
                     /gene="lptE"
                     /locus_tag="P5558_07945"
     CDS             18492..19061
                     /gene="lptE"
                     /locus_tag="P5558_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRY24278.1"
                     /translation="MSWSRPLARRGCWRLGAAALLPLAAGACGFRPLYGGAVGEEVAA
                     ELASIELVAPATRVGQLLKNRLLDDLNPAGATVARRYRLIVELSRTQTALAIQLDETI
                     TRYDLTLAAFFSLHDLEVDGAGMPAAEGEAGPEPAYRSALRRVASYNVVTDPFATLMA
                     EQDAELRAATELSRGIRTLLTLHFQGGPA"
     gene            19058..20080
                     /gene="holA"
                     /locus_tag="P5558_07950"
     CDS             19058..20080
                     /gene="holA"
                     /locus_tag="P5558_07950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRY24279.1"
                     /translation="MKVQTGSADGFCRRPDPAIGVVLLYGPDQGLVADRRRQLVEAVL
                     GAADDPFRLTELEAERVTAAPGLLLEEAQALSLVGGRRIVLLRQATDTMAKPLAGLLG
                     QGGHAALVIVEAGELGAGSALRRLAEKDRAAAAIACYRIDGAGLASQIRDHLRGLGLS
                     AEPEAMAYLVEHLGGNRELTRAELEKLALYKGEDRAARITLDDVAAVVGNSSALYVDA
                     LVWACLVGRPGTAEPILDRLLGEGQAAVRLIRAISSALLRLLPLAARAARGEAPAAVV
                     QGARPPVHFSQRASMQRALESWRAAGLEAALALALEAERRCKRARAPERLLLRRLVHE
                     IALLPR"
     gene            20150..21148
                     /locus_tag="P5558_07955"
     CDS             20150..21148
                     /locus_tag="P5558_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24280.1"
                     /translation="MLRYVLHRLIVMIPTVIAISVVTFVIIQLPPGDFLSAQISELQA
                     QGEGVQTERIAYLRELYGLERPLWQQYLQWAWGLLHGDLGWSFEYDRPVTEVIGDKIF
                     LTVVISLATVIFTWIVAFPIALYSATHKYSWGDHGLTFLGFLGLATPNFLLALVMLYF
                     ANVWFGTSIGGLMDPVYLDQPMSWGKFVSVLQHLWIPVIIIGTSGTAGMIRRLRANLM
                     DELQKPYVVTAKAKGLPPGRALVKYPLRMSLNPFIADIGNLLPDLISGSALVAVVLSL
                     PTTGPVLVRALQSQDMYLAGSFLMLEAFLVVIGVLISDLLLAWLDPRIRFSGGQAR"
BASE COUNT         2998 a         7117 c         7573 g         3462 t
ORIGIN      
        1 tccaggtttc gccgtcgtcc cggctctcga agaggccggc gggctcggcg ccggtgagga
       61 ggcggccgtg ggcgtgggcc agggaccaga cggcctttat tttgtcgtgt gcctcggggt
      121 aggcgaggcc gtcggtgaat ttctcgaagg tggcgccctg gtcggtggag cgccagacgg
      181 cagcaccgcg ccaggcgtcg ccgccgccgg cccagatcgt gccggtctcg gggtcggcca
      241 ctaggtggtt cgtgggctgg gcgtcgcaga gggggccgtg gacggtccag tcttggcggc
      301 cctggttgct ctcgaggatg aaggcgccct tgccggtggc gacgaggacg aggagtcttt
      361 ttgccattcg agcctccttg atccctttcg gttcagtcgg tgtagcggtc gtggtggcgg
      421 acccagtcca tcgcggtggt ctcgttgcgg cccttgggtg tcaggtcgag gaagttgtgg
      481 acgcccagga ggatgtcgag gccgcgggcg taggtcgacc aggtgtggaa gacggtgccc
      541 tcatcgtcct tcgtgaagac gctgacgccg ggcatctcgc cccagacgcc ggggagggtc
      601 gtgtagttgt agggctgcgt gccgcacgcc ttgtcggcgt cggtggcgga gacctggaag
      661 tcgaagttga aggcgctgcc ggccgaggag acccagggga agcgccagcc catgcgggcc
      721 ttgaagggca ggagctccgc gagcggggcc atggagacgg cggcgaggct gacgtcgtgg
      781 tggcggaggt gctgctcgat gccgtcgaaa tggtcggcga ggagggagca gctcttgcag
      841 ccttcgctcc agccgggggc gaacatgaag tggtagagga cgagctggct gtttcggccg
      901 aagagttcgg cgaggccgag ggggccgtcg ggaccctcga agcgatagtc cttctcgacg
      961 cgctcccagg ggagggcgcg gcgggcttcg ttgacgcggt cgtgggcgcg ggtgagcgcc
     1021 ttctcctcgg cgaggagggc cttgcgggct tcgagccatt cggcatggga gacgactggg
     1081 tgggcaactg cggtatcggg catggtggtc gtcctaggac aggctggcga ggtgatcgtc
     1141 gaggcgctcg aggcagctgg tccagccgcc catgtggctg tcgcggctca gggcgtcgcg
     1201 gaacggtgtc tggtggaagg tgaggcgggt cttgtcgccc tccggcttca gggtgacggt
     1261 gatcagggtc aagagcgcgt ccggggattc ccaggcgaag ctgaagacga ggcgctcgac
     1321 cgggtcgatc tcctgccagg tgccgcccat ccagtggtcc tcgccctcgg gcgagcggat
     1381 gcaggcgcgc cattggccgc ccgggcggat gtcggcctcc atcgcgggca gggtgaagct
     1441 cttcggaccc caccagcggg cgagctgggc ggggtccgtc caggcggcga agacgcgatc
     1501 tcggggggcg tcgagggtgc gggtgatgcg cagctcgatg ggggcttcgg tagccgtggc
     1561 ggtggccatg ggtgggtgtc cttgcggaag cgactggcgg tggcgcggcc ggccggagag
     1621 tcgcggccgc acgctgcggc ccgccggtgt cgacgggcgg gccgccggcg ccggccggcg
     1681 gcgcctcgat cagtcctgcg aggccggggt catccagccg atgccggcgc cgccgggctg
     1741 ctgcaggatg gcgatgcgcc cgacccccgg cacgtcgaag atcgggcgct tgagctcggc
     1801 accggcgctg acggccttcg ccacgcgggc gtcgacgtcg tcgacggcga gataggccat
     1861 ccagaattcc ggcaggccct cgtgctccgg ctttctgagc tggaacatgc cgccggcata
     1921 ggcttctccc attctggcga cccagtaggt cccgtcgggc atcggcatgg attcgaaggt
     1981 ccagccgatc gtgtcggcgt agaacttctt ggcgcgctcc acgtcccagg tcagaagctc
     2041 gttccagtgg aaggcaccgt gcgaccaggg catcgtcgtt cctcctttgg tgatccgggg
     2101 tttcaagccg aaagaaaggc gtcgagcttg tcgagggagc cggtccagcc ctgcgcgtgg
     2161 cggccacggg tcgcgtcatc ggcgaactgc tcgtggacca gcgtcaggag cgtgccgccc
     2221 tgcacctccg cgaggcgcag cgtgacgacg gactggcgct cgggcatgct cttccaggcc
     2281 caggtgaaga ccagcctctc cggcggcacc acctcgcggt aggtgccgcc gacctcgtgc
     2341 cgcccgccct cgtcgtcgag catgacgatc cggtagctgc cgccctcgaa ggcggcgacc
     2401 tccgcctcca ggaccgagac ccgggccggg ccgaaccagc ggctcagctt ttgcgggtcg
     2461 gtccaggcgg cgaagacggt ttcccggggg gcgttgaagt gtcgcctgag ggtgaggctc
     2521 ggtctggtgt cggtggcgga catggggctt cgctttcttc ctcgaggaag gcggccaggc
     2581 gatcgaggct ctcggaccag aatcgctggt agcgctcgag ccagtcgatc gcctcgcgca
     2641 tgggtgccgc attcagccgg caggtgacgg tgcggccgac cttgcggcgc tcgaccaggc
     2701 cggcatcgga gagcacggcc aggtgcttca tgacgccggg cagcgagatc gcgaacggcc
     2761 gggcgagctc gctgacgctc agcgattccg cctcgccgag ccgggcgagc agggcgcgcc
     2821 gggtcggatc ggcgagggcc gcgaaggtgc gatcgaggtc gggcgactga aagttaacca
     2881 tgaggttaag tgtatggtca tgcgggatgg acgtcaagcg tccttcgttc agtcgggatg
     2941 gattttcccg ggccagcgct gacggcccgg attcgtcggc tcggtcgaaa ttcagcgagg
     3001 gagaaagaag gtctgcgggg gctttggccg ttcgccacgt gccagggcgt ctagatacgc
     3061 ttcgacgtcc ttgtggtggg cgaatggtcc gccagcgtcg gcttcgatca cggcttcggc
     3121 aatcgcttgc gcatgccaag cgtcgagatc gagaaaagcc tggatggcct ctgcaacgac
     3181 atcgtcacga tggcgctcgg tgcggcgggc gacttcgtcg aggcgctcgg cgagcttctc
     3241 ggaaaggctg atccggatcg tcgtggtggc gctcatggcc gggtccgcgg gcggttgtgc
     3301 aagcatgatg ataacacgcc gctggccgcc ttgcgaaccc ggccgagccg tcagcccgtc
     3361 agctcagctg atgccgccga ggctgacata cttgatctcc aggtactcgt cgacgccgta
     3421 cttggagccc tcgcggccga ggccgctttc cttgacgccg ccgaaggggg cgacctcgac
     3481 ggcgatgacg ccgtcattca cgccgatgag gccgacctcc atcgcctcgg cgacgcggaa
     3541 gacgcggccg atgtcacggg cgtagaagta gccggcgagg ccgacgtcgc tgtcgttggc
     3601 gagcttgatg acctcctcct cggtctcgaa cttgaagatc ggggccaccg ggccgaaggt
     3661 ctcctcgtgg gcgatcttca tgtcggcgtt gcagttggcg agcacggtgg gctcgaagaa
     3721 gaggccgccg agctcgtgcg gcttgccgcc gagggtgagg acggcgccct tggcgaccgc
     3781 gtccttgacg tgctcgctcg acttgtcgag ggccgcctgc tcgatcagcg ggccgacctc
     3841 cacacccttc tcggtgccgt ggccgacctt gagctcggac gcggccttct tgaacttctc
     3901 gacgaactcg tcgtagacgc cggcctggac gtagatgcgg ttggtgcaga cgcaggtctg
     3961 gccgcagttc ctgaacttgc agaggatggc acccttgacc gccgcgtcga tgtcggcgtc
     4021 gtcgaagacg atgaaggggg cgtggccgcc gagctccatc gagacccgct tcaccgtgtc
     4081 ggcgcactgc ttgagcagga gcttgccgac gtcggtcgag ccggtgaacg agaccttgcg
     4141 cacggtcttg ttcgaggtca gctcgccgcc gatgacgcgg gcactgccgg tgacgatgtt
     4201 gaacacgccc ttgggcatgc cggcccgctc ggcgagctcg gccagggcca gggccgaata
     4261 gggcgtcatc gaggcgggct tgacgacgaa ggtgcagccc acggcgaggc ccggcgcgca
     4321 tttgcgcgtg atcatcgcgt tggggaagtt ccagggcgtg atggcggcgc agacgccgac
     4381 cggctgcttg atggcgagga tgcggcggct cgccacggtc tgcgggatga cgtcgccgta
     4441 gacgcgcttg gcctcctcgc cgaaccactc gacgaaggcg gcgccgtaga ccacctcgcc
     4501 ggtcgcctcg gcgagcggct tgccctgctc gcgggtcatc aggtagccga ggtcctcctt
     4561 gctcgccatg atcaggtcga accacttctc gaggatcgcg gcccgctcct tgccggtctt
     4621 ggcgcgccag gccggcagcg cggcctcggc ggcggcgatg gcgcggcggg tctcgtcgac
     4681 gcccatgtcg gggacgctgc cgaggcgctc gccggtcgcg gggtcggtga cgtcgaaggt
     4741 cttgccgctg tcggcggcga cccattggcc gtcgatgtag cagcgctcga tgaacaggct
     4801 cttgtcgctg agcgcgtttt tgatgtcctt cagtgcgacc gccatggtcg tcctccctct
     4861 cgtgtgatga acaatggtcg atgacccgcg gctcaggccg cctcgatgcc tcttgccaga
     4921 taccggctca tgcgtctgga gaaggcgccc ggatcgggca ccggctcgcc ctcgatgata
     4981 cgcgcctgat cgagcaacag aaaggccagc tcgtcgagat cgggtgtcgc cgtcgggtcg
     5041 tcgaccatct ggcgcagccg gccgaccagc gcgtgctcgg cattgatctc gaggatgggc
     5101 ttcgtgccgc cggcgaactg ctcgtggccc ttgagcatcc gaccgagcct gagatccatg
     5161 ccgccgtcgt cggcgacgag gcagacggcg ctctccttca ggcgcttcga cgcgcgcacg
     5221 tccttgacct gctcgccgag cgccagcttg agccgggcga tcagcttgtc gagcccggcc
     5281 tggtcggctc ttggcgtctc gggcgagtcc gcacccttga tcgacgacag atcgacatcg
     5341 ctcctggtca gcgagtggag cttcttgccc ttgtactcgc cgacctcggg cagccagaac
     5401 tcgtcgaccg gatcgtcgaa gagcagcacc tcgacaccct tggcgcgggc catctcgagc
     5461 tgcgggctcg agcggagcgc ctcgatgctg gtgccggtga tgtagaagat gtcggcctgg
     5521 ccttccttca tccgctcgac atagccgtcg agggtcgtcc agcccttgcc ttcggtcgag
     5581 cggaagcggg cgatctcgag gagcctctcg cggttctcat ggtcctcgaa gagaccctcc
     5641 ttgagcaccg gcccgaaatc ggcccagaag gcctcgtagc catcctcctt cgccttcttc
     5701 gccagctcgt cgaggacacg cctgaccagc accttacgca tcttctgcac gatggcgccg
     5761 tgctgcagag tctcgcggct gacgttgagc tggaggtcct cgctgtcgag cacgccggcg
     5821 acgaagcgca ggtaacgcgg catcagggcc tcgacgtcat cggtgatgaa gacccgcttg
     5881 acgtagagct tcacgccatg ccgccgcttg gggtcgtaga ggtcgaaggg gcgcatcgac
     5941 gggacgaaga ggagggcggt gtagctgagc tggccctcgg cggtgaaatg cacgcgcgcg
     6001 aagggatcgt cgaaggcgtg ggcgacgtgg tggtagaatt ccttgtactg ctcgtcggtg
     6061 atctcgccgc gcggccgggt ccagagcgca ccaccctcgt tgaccttctc cggcgccttc
     6121 ggctcggcct cggcctcctt gtcatcggcc ttgggcttga cctggaggag gatggggaaa
     6181 gcgatgtggt cgctgtagcg gcggatgatc tcgcgcaggc gccattcgcc gagaaactcc
     6241 ctggcgtcct ccttgagatg cagggtgacc gtcacgccgc gcgggcccgt ttccggcgcc
     6301 tcgctgacgg tgaagccctg gcggccgtcg gagacccaga gccagctcgt ctcctcgccg
     6361 gccttgcggc tctgcacgac cacccggtcg gcgaccatga aggcggcgta gaagccgacg
     6421 ccgaactggc cgatcagctt gacgtcgccg ttcttctgct gctcggccgc ctcgaggaag
     6481 cgggcggtgc cggagcgggc gatggtgccg agattggcga ccagctcgtc ccggtccatg
     6541 ccgatgccgt tgtcgcggat ggtcaggagc gccgcgtccg gatcggcgaa gatctcgatc
     6601 ttcaggtcgg tatcggtgcc cagcagctcc ggctgggaga gcgaggcgta gcgcagcttg
     6661 tcacaggcgt ccgaggcgtt cgagacgagc tcgcgcagga agatttcccg ctccttgtag
     6721 agcgagttga tgaccagatc gagaacgcgg ccgacctcgg cctcgaaggg ttgaaccgta
     6781 tcgctcatgg gcacctgaac ctgcctgggg tcgaaagacg aggaatgact tgcccggaac
     6841 gtgggcaagc ccggggccgg cttcaagtcg ccgcactgcc tggcggctag tcgaggcgca
     6901 gggccggcgc catccggccc gacgcgaccc accatatcta gtggttgttg cggtgcaacc
     6961 gccataatcc tgacatagca tgtcgtgcac ggggcttcga aaccacttgc caaacgcggc
     7021 gcccgcgcga cattctcaac tcatgatgcg cgagccgctt tcctctctcc gcatggcggc
     7081 aggcataggc cgcatggtgg cggatccagg ccgcattgcg gcggtcctcg gtccgaccaa
     7141 caccggcaag acgcattatg ccgtggagcg catgctggct catggcagcg gcatgatggg
     7201 gttcccgctc aggctcctcg cccgcgagat ctacgatcgg gtcgtccagt tgcgcgggcc
     7261 atcggcggtg gcgctcctga ccggcgagga gcagatcggc agcgacacgg cccgctacgt
     7321 catcgccacg gtcgaggcga tgccgatcca gcgccgcttc gccttcctcg ccgtcgacga
     7381 gatccagctc tgcgccgatc gcgagcgcgg ccatgtcttc accgaccggc tcctgcatgc
     7441 gcgcggcgag gaggagacgc tcttcctcgg ctccgacacg atccggccgc tcctgaagca
     7501 gctcgtgccg gaggccgaga tcgtcacccg gccgcgcttc tcgatcctcc gccatgtcgg
     7561 ccccggcaag ctcaaccggc tgccgcggcg gagtgctgcg gtcgccttca ccgctgccga
     7621 cgtctacacc ctggccgagg tcatgcgtcg ccagaagggc ggggcggccg tcgtcctcgg
     7681 tgccctgagc ccgcgcacca ggaacgccca ggtggccatg taccagtcgg gcgaggtcga
     7741 ccacctggtc gccaccgacg ccatcggcat gggcctcaac atggacctcg tccatgtcgc
     7801 cttcgcccag ctccacaagt tcgacgggcg ccacgaccgc cggctcacgg ccgccgagat
     7861 cggccagatc gccggccggg ccgggcggca catggcggac ggcacgttcg gcacgaccaa
     7921 tggcagcggc ccgctggacg accgtatcgt cgaggcggtg gaatcccaca atttcctgcc
     7981 gctcaagcgg ctgcgctggc gcaacagcga cctcgatttc gccagcctgg aggccctgaa
     8041 cgagagcctc gacgccgacc cgccggcacc gggcctggtc aaggtcaggg acgcgctcga
     8101 cgaccgcagc ctcggcatgc tggcccggcg cgacgacatc cgcgcccggg cacgcgggcc
     8161 ggccggggtg cgcctgctct ggcaggtctg ccagatcccg gatttccgcc ggacgttgac
     8221 ggacgcgcat ctgcagctcc tgggtatcgt cttccgccat ctgaccgacg gtcccgcggt
     8281 gctgccggcc gactgggtcg gcagcatgat cgcccggctg gatcgcatgg acggcgacat
     8341 cgacacgctg gtcgggcgga tcgcccatgt ccgcacctgg acctacctct cgcaccgggc
     8401 cggctggttc gacgatccct tgcactggca gggggtggcg cgggcggtcg aggaccggct
     8461 gtcggatgcg ctgcacgagc ggctgacgca gaagttcgtc gaccggcgga cggcggcgct
     8521 gctgaagagc ctgcgcgagc ggggcgagct cgcggccgag atcgagcccg gcggcgaggt
     8581 cgtggtcgac ggccattcgg tcggcacgct cgagggcttc gtcttcacgc tcggggcgag
     8641 cggtctcgag accgagcacc ggctctttgc cggtgcggcg cggcgggcgg tcgcccgtca
     8701 tctcgaccgg ctggcggccg agctggtgaa ggagccggac ggcgccttca ccatcatcgg
     8761 cggcgagctc gtctggcgcg acgcggtcgt cgccaccatc cggccgggcc cggtgcggct
     8821 gcggccgacg gtggcggtga ccgccacgga cgagctgacg acgaaggcgc ggctcgccat
     8881 cgagcgccgg ctcgaggcgc ggctcagggc ctggctcgag gatcgcctgg ggccgctctt
     8941 cgccctcgag gccgcgtcac ggagcgaggc gctctcggcc gccgcgcgcg gcatcgccta
     9001 ccggctggtc gaggccttcg gcatgctcga tgcggccgag gcggccggcc tcgtccgcca
     9061 gctcgacagt gccgaccgaa aggcagtggc gcggttcggg gtgcggctcg gcgtgcatca
     9121 catcttcctt ccggccctgc tgaagccggc tgcacaggag gccggcgccc tcctcgtggc
     9181 gctggcgagc ggcaccgccg cctggcagcc gaccgccggc cgggtggcgt tgcgcggcga
     9241 cgagctgccg gcggacggca tggcgctggc ggccctcggc tttcgccgga tcggacggct
     9301 ggcgctgcgc gtcgacatgg tcgagcgggt cgcggcgcag ctccgccggc tcgcccgggg
     9361 cgaggcgcag ttctccctgc cggccgagct ggtgagcgct accggtctcg gccgcgacga
     9421 gttcatggag cttctgcccg gcctcggctt tcgggccatg gcaccggcgg ccgacgacgt
     9481 cccggcgggt gccatcaaca tccgccgggc caagccgcgg cgccggccga gcgccgcgca
     9541 ccgcaaggag cgcgcccagg cggggccgag ccagtcgccg tttgccgtgc tcgcccggct
     9601 caaggtcgcc gccggtgcct gaggcgcggc ccgggcgatg acggaaggcg ggctgcgcct
     9661 cgacaagtgg ctgtttcacg cgcgcttcat caagcagcgg gtgctggcgg tggagctggt
     9721 gcgtcgccgg cggatccggc tcaacgaccg gctggtggag aaggtgcaca gcacggtgcg
     9781 gccgggcgac gtgatcacgc tggtggcccc gtccggggtc gcggtgctga aggtcctggc
     9841 cctgggcgag cggcgcggcc cggcgccgga ggcgcggacg ctctacgagg agctttccta
     9901 gcgcggcggg cgggcgccga agatggcgct gccgacgcgg acatgggtgg cgccgaagcg
     9961 gaccgcgtcg gcgtagtcgt cgctcatgcc gatgctgacc tgctcgaggc cgctgcgttc
    10021 cgcgagcttg accagaaagg ccgtgtgcag ggcgatgtcc tcgtccgccg gcgggatcgc
    10081 catgaggccc cggacgtcga ggccgtggct ctcgcgacag agccgcacga aggcctccgt
    10141 ctcctccggc atgatcccgg ccttttgcgg ctctttcccc gtgttgacct gaacgaagca
    10201 gcgcaacgcc ttgccctgct tcgtcatttc cttctcgagg gcggcggcga gacggggccg
    10261 gtcgacggtc tggatgacgt cgaagagggc cacggcgtcc tttgccttgt tggtctgcaa
    10321 ggcaccgatg agatgcagct cgacctcggg gtagcgggca cggagcgccg gccagcggcc
    10381 ctgcgcctcc tgcacgtagt tctcgccgaa gacgcgctgg cccgcctcga gcgccgcctc
    10441 gagccgctcc agcggctgcg ccttgccgac cgcgatcagc gtcaccgccg ccggatcgcg
    10501 cccggcttcg gcggccgcct cgaggatccg ggcgcgcacg gcggcgaggt tggcggccac
    10561 gtccaccgtg tggtccaggg cttctggcat ggctttgggg ccccttggtg ctgtcgctgc
    10621 atcgctgcta cacgtgccgg ccgatgacga ccagtcaaga ggtgtcccga tgagcatggg
    10681 cttcgaccag atcctcgcac cgctcggcgg ggagaccttc ctgcgcgact atctcggcca
    10741 gcgcccggtg catatcgagg gcggggcgga gaagttccgg gagctgatga gctggtcggt
    10801 gctcaatcgc ctgctcggca tgagcacgat ctgggacgcc gagcggctgg tcatggtgct
    10861 cgacaaggag gaggtgccgc cggccgccta tctcggccgc gacccggcga cgagcggtcg
    10921 gccggccgcc gatccgtcca gggtcaagca atacatcaag cagggtgcga cgctggtcct
    10981 caacgacatc gaccagctca ccccggagct cagcgccttc gcccggacga tggagacggc
    11041 gctgggcggc aaggtgcagg gcaacctgta ccagtcctcg aagcggcgcc aggggttcaa
    11101 ggtccactac gacacccatg acgtcttcgc cgtgcattgc gagggcgaga aggtctggcc
    11161 ggtgttccag gggcgggccg aggacccgat cgcccacccg atcttcaaga acgtgccgca
    11221 ggaggcgcac gagaaggcca agggcgagct ctggaaggag gtccggatga agcccggcga
    11281 cctcctctat ctgccgcgcg gccagtacca ctatgccctg gcggatgacg gcggctgcat
    11341 ccacatcgcc ttcggggtga cctacccgat cggcatcgac gtgatgagct acctcttcga
    11401 gcggatgatc acggacccgc tctgtcgccg caacctgccg cgcggcgatg ccggcgcact
    11461 ggcggcgcat ctggggacgc tcggcgagcg gctcggcgag gtgctggcgg agccgcgcgc
    11521 actggccgac atgcaggcct tccaggcggg ctatcgctac cagcgcggga gctacgactt
    11581 ccccgagctg ctcgacccgg cgtccttgcg ctacaccgtc aacggcaagg gcgtccggct
    11641 gatcgagcag cagggccgct tcggcctggt caaggagggc agccgacagg ccgtgggggt
    11701 gccgggcgag gtgagcggca tggttgcctg ggtgctgaag cgaccgggtt tcagccgcgc
    11761 cgagctcgac gaggccttcc cggccgccga caaggccacg ctcgacaagt tcatcgtcga
    11821 catggagcgc atgctgctga tcgcggtcgt ggcctgaggc tgctgccgcc ttggtcgatg
    11881 tcttcttctg ggtccagcat ctcctgggca tcggccatct cgcccgggcc gcgaccctgg
    11941 tccgggcgat gcgccggcat ggcctcgagg tcacgctcgc cagcggcggg ctgccggtcc
    12001 cttcactcga cctcgagggc ggcgagcttc tgcagctcgc gcccgtgcag tgcggcaccg
    12061 gcgatttctc caccttgctg gatgccgccg gcgagccggt cgacgaggca ttcaagcagc
    12121 gccgggcccg ggcgctggtc gaggccttcg aggtgcggcg gccgcgtctc ctgctgctcg
    12181 agatgttccc cttcggccgg cggcagatgc gcttcgagct ggagccgctg ctcgaggcgg
    12241 cacgggcgct cgtgccccga ccgccgatcg tgagctcggt gcgggacatc ctggtgcgga
    12301 agagcaggcc cgagcgggag gccgagatgc tggcccgggc gctgaccttc gacgccgtgc
    12361 tggcgcatgg cgatccggct ttcgcgccgt tcagcgccac cttcccggcg agcgccaggc
    12421 tcggcgaccg gctgatcgag accggctacg tggtggaccc gctgccgccg gacgacggca
    12481 ggagcacgga tgggcagggc gagatcatcg tctcggccgg cggcgggcgg gtcggcgagc
    12541 gcctgctcga gaccgccatc gaggcgagcg ccttcgcccg gggcggtccg gcctggcgtg
    12601 tgctggtcgg ctggcaggtg ccggaggccg acttcgcggc gctggcccgg cgggcccgcc
    12661 cggggctggt gatcgagcgg gcccgccctg attttcgcac cctgctcggc cgctgcgcgc
    12721 tctcggtcag ccagggcggc tacaacacgg tggtcgaggt gctggccgcc ggctgccgtg
    12781 ccgtggtcgt gccttatgcg gaagacggcg agaccgagca ggcggagcgc tgccgccggc
    12841 tcgccgagcg cggcctgttg catccgctcg ccgcccggga actcgacccg ccggggctcg
    12901 cccggctggt cgcccaggtg ctggcgagtc cggcccggac cggaccgccg ccccgattgg
    12961 acggtgcgga cgtcacggcc cggttgcttc ggcggctgag ccgtcctacg ggcaggtgag
    13021 aacgggctaa acgcctgcaa tcccggcttg ttgcaggccc gacacagtga aaccccaatg
    13081 gcggatgccg ctatggcttc ggcttgcgcc gtgctcgacc caatgaataa cacggagacc
    13141 ggatgacggg gcgccttcag cgtcccatcc gcggagccga tcggcggcgc cgcccgatga
    13201 acccgaaggc gatcccactt tggggtgacg aggagacaaa aaagatgaac ttgcgtaatg
    13261 ctttgttggg cacgaccgcc atgatcggcg cggctgccct cgtcgtgccg tcgaccgcct
    13321 ccgcgttcga tgtcaacatc aacggcttcc tgaacttcaa cgccgtcggc ggtgacacct
    13381 ccgaagccct tggccagagc gcgtccagct tcgatttcgt caccgacacg gaaatccacg
    13441 tccagggcgt gcagaccgac gacgagaccg gcatccgcta cggcatgacc gtcgagttcg
    13501 aggcggaccc gcagaccagc aacaacaaca acatcgacga ggcctggatc ttcatcgacg
    13561 gcagcttcgg cggcatccgg ctcggcaacg aggacggcat cgtcgacaac tcgaagctcg
    13621 gtgcctacac gatcgcggcc ggcacgggcg gtatcgacgg tcagggcgtc gtcggtgcag
    13681 tgaagttcgc gccggtcaac tccggcgaca gcaccaaggc ccgctactac tccccgacca
    13741 tcgccggctt ctcggtgggt gtcagctaca cgcctaacgg cggcgtcgag ggcaaccagg
    13801 tcgccgacac gcagagtgtc cagtacaccg acatgttcga aggtggcctg gtctactcga
    13861 acagctttgg cagcctcgat ctgctcgcct ccgcggtcgg catctacgcc accggcatgg
    13921 acagcaacac gggcggcaac aacttcgacc gcgacgacta taccggctac aacttcggtg
    13981 cggtggtcgg cttcgccggc ttcgaggttg ccggcagcta ctggggtgag caggacgcgc
    14041 ccgcgggcaa caacgccaac aacagcgtca atcggaacgg cttcaccgcc ggcgccgcgg
    14101 cgtccttcgg cccggccgat ctctcggtca cctatgccaa ggtcaccaac ggcggcggcg
    14161 gtgtcggcga gggcgacaat gtggtgctca gcgccagcat gggtctcctg cccggcatgg
    14221 cgctgcaggg tgacgtttcg tggttcgacc gcgacgccgg tggcgacgac gacggtgtga
    14281 ccggcgtcgc tcgtgtccgg gtggccttct gatcggcccg atcgatctga cgtagcggaa
    14341 cggcgggctt cggcccgccg ttttgccgtt ccttgcgcgg ccgggccggt gtcgcggcca
    14401 tgcgtcggca gaccgggcgc gcctgcaaca gatgcgtcca atcgggcgtc ccgtttcgcg
    14461 aaacggttgc gccgcgaacc ggtaaagccg tatacgatct tggttgctgg tgcgaccgga
    14521 gttgatgatg cgaccttgtc gtcctgcccc cgttctgccg atcctggctg tcaccgtgct
    14581 cgtcggactc gttgccggcg ggccggctgt tgccatggac ctggtgccgg gcggctacgg
    14641 cgtcgccgat ccggatgcca tgcaggccgg gcagggcaat gaagcgcggg tcggcacgac
    14701 ggggtcggtc gccggcctga ccttcgattt cacgccgcgc gccatcggtt cgctgatcaa
    14761 tccttccatg ctcgatccct ctacggaggc tgccgggcag cagcccttgt cgctcacgct
    14821 cggcgtgcag aatcgctatg cggagggttt gcgtctgggt tccgtcgggc tggtcgagcc
    14881 gtcgaccgaa gccacccatg gcctggcgct gggcggtgcc ttcgacatca acgactggca
    14941 gctcaccggc agctacgggc gcaccaatct catgggcgag gcggcggacg tgttcgccgc
    15001 cggcatcggc tacggcccga tgagcgcccg tctcgtctac gggcatgtgc cgcgcaccac
    15061 cggcccgtcg ggagatgtgc tgatgttcag caccgatctg gccgcgtggt catggctgac
    15121 cctcgagggc gatgtggcgg tcagcgacag cctggccgag gagccactga cggtcggccg
    15181 ggttggtctc cgcctgaact tctgattccg ccggccgctc gcgcggccgt gtcatctccg
    15241 ccatgcgggt ctggctttgt cgttcgggcg tgctatgacg tttcgacacg gcgtcgatgc
    15301 tcgatgagac gatggaaatc cccaagtcca tgacccagaa atccttggcc gcgcgaggcc
    15361 gttggctgat tctcctggcg gtgctcgccg tgaccgcctg cgagggcgtc gaggcggagc
    15421 cgccgccgca aaccacgacc ggcacggcac gggatcagcg tgaccgggag cgtttcggca
    15481 ccatcacggg cgaaggcccg ctcatatcgt tcagcaccga tcgggctcgg gcgccgaacg
    15541 aaggcgtggg tggcgggatc ggggtcaacg ccttcctctg ggaagcgacg ctggatacca
    15601 tcaacttcat gccgctggcc tcggccgatc ccttcggcgg cttgatcatc accgactggt
    15661 tccagccggc cacgacgccg ggcgagcggg tcaagctgca cgtgcagatc cgcgatgcgg
    15721 cgctccgggc cgattcggtg accgtgtcgg tgtttcgcca gcagcgcgac ccggccggcg
    15781 actggctcga cgcgccggcc gatgcgacga ccgcgcgcga gatcgaggac cacatcctca
    15841 cccgggcgcg cgagcttcgc gtcgcttccg ccgcggcggg ctgacgacgt tcaccagcgc
    15901 cctgccgggc gttctttctt ccccgaccac gaggccattg atgtcgcgct acccgtttcg
    15961 tgccaccgag cagaagtggc agcaggtctg ggacgagcgg cgctcgttcg aggtcgggat
    16021 cgaccggacg aagccgaagt actacgtgct cgagatgttc ccctatccct cggggcgcat
    16081 ccacatgggg cacgtccgca actacaccat gggtgacgtc gtggcgcgcg cccggcgggc
    16141 ccagggctac aacgtgctgc atccgatggg gtgggacgcc ttcggtctgc cggccgagaa
    16201 cgcggccatg cagaagggcg tgcatccggg tgtctggacc tatgccaaca tcgccgagat
    16261 gcgcagccag ttcaagcaga tggggctggc gctcgactgg tcgcgcgagc tcgcgacctg
    16321 cgatgtcgag tactaccggc acgagcaggc gatgttcctc gacatgctcg agcgcggcct
    16381 cgtctaccgc aaggagagct gggtcaactg ggatccggtc gaccagacgg tgctggccaa
    16441 cgagcaggtg atcgatggcc ggggctggcg ctccggtgcc gagatcgagc gccgcaagct
    16501 caaccagtgg gtctttcgga tcaccgcctt cgccgacgag ctgctcgccg ccctcgacga
    16561 cctggagcgc tggccggaga aggtccggct gatgcagacc aactggctcg gccgctcgga
    16621 gggtgccctc gtgcatttcc cggtccaggg tcgggaggag ccgctcaccg tcttcaccac
    16681 gcggccggac acgctctggg gtgcctcctt catggcgatc gccccggatc acccgctggc
    16741 ccaggagctg gccgaggccg accctgcgct cgccgccttc atcgaggagt gcctcaagct
    16801 cggcacctcc gaggaggcga tggagcgggc cgagaagaag ggcttcgaca ccggcctgcg
    16861 ctgtcgccat ccgctcggtg cgacgcctga tcttccggtc tatgtcgcga atttcgtgct
    16921 gatgggatat ggcacgggcg ccatcttcgg ctgtccggcg cacgaccagc gcgatctcga
    16981 gttcgcccgc aaatacggcc ggccggtcac cccggtggtg ctgccggagg gagccgatcc
    17041 cgccagcttc gcgatcggcg cggaggccta tgtcggcgac ggcaccatct tcaactccgg
    17101 catgctcgac gggctcgccg tcgctgaagc caagcgccgc atcatcgagc acctggcgac
    17161 cgaagggctc ggcgagcggc aggtgacctg gcggctgcgg gactggggcg tctcgcgcca
    17221 gcgctactgg ggctgcccca tcccggtcct ctacacggag gatggcgaga tcctgccggt
    17281 gccgcgcgac gccctgccgg tgcggctgcc cgacgacgtc gatttcagcg cccccggcaa
    17341 cccgctcgac cggcacccga cctggaagca cgtcaccctg ccggacggtc ggcgggcgac
    17401 gcgcgagacc gacacgttcg acaccttcgt cgagagctcc tggtactttg cccggttctg
    17461 ctcgccccgg acggaggacc ggccgttcga tcgggcggcg gtcgactact ggctgccggt
    17521 cgaccagtat atcggcggcg tcgagcatgc ggtgctgcat ctgctctatt cccgcttctt
    17581 cacccgcgcc atgtcggcct gcggccatct cgacctcgcc gagccgttcg ccggcctttt
    17641 cacgcagggc atggtcaccc accagaccta ccgcgacccg cacggccgct ggctgcagcc
    17701 cgacgaggtg accaggggcg ccgacggcga gctggtgacg gcccggggtg cccgcccggt
    17761 cagcgtcggc cgggtcgaga agatgagcaa gtccaagcgc aacacggtcg accccgcggt
    17821 catcatcgag gcctacggag cggacaccgc tcggctcttc atgctctccg acagcccgcc
    17881 cgagcgcgac ctcgaatgga ccgaccaggg gatcgacggc gcctggcgct atctcaaccg
    17941 actctggcgg ctggtcgacg agcgccgtgc cgagctgccg gcggccggcg gtgccgcgag
    18001 cgcgccggcg agcgaggccg cccaggagct caagcggctg gtgcatcgca gcattgccgc
    18061 cgtgaccacc gagatcgacc ggctgcactt caacaaggcg gtggccctgg tgcgcgagct
    18121 ctcgaacgcc atcgagggct acgaggcggc gggcgagaac gaccggcagg tgctgcgcga
    18181 ggcgctcgag acgttggtgc agttgatcgg cccgatgctg ccgcacctcg ccgaggagct
    18241 ctgggaacgg ctcggccacc ggacgatcct cgccgacacg ccctggccgg tggccgatcc
    18301 ggcgtggctc gcagcggcga gcgtcaccat cgcggtccag gtcaacggca agcggcgggc
    18361 cgcgatcgac gtgccggtcg gcagcgacaa gacctcggtg gaggcagcgg cgctcgccct
    18421 ggaggccgtc aagcgggcga tcgacggcca gccggtgcgt cgggtcatcg tggttcccga
    18481 ccggatcgtg aatgtcgtgg tctaggccgc tcgcgcgccg gggctgctgg cgcctgggtg
    18541 ctgcggcgct gctgccgctc gcggccggcg cctgcggctt tcgcccgctc tatggcggtg
    18601 cggtcggcga ggaggtcgcg gccgagctcg cctcgatcga gttggtggca ccggccacgc
    18661 gggtcgggca gctcctcaag aaccgtctgc tcgacgacct gaatccggcc ggcgcgacgg
    18721 tcgcccgccg ttatcggctg atcgtcgagc tgagccgcac gcagacggcg ctggcgatcc
    18781 agctcgacga gaccatcacg cgctacgatc tgacgctcgc cgcgttcttc tcgctgcacg
    18841 acctcgaggt ggacggcgcc gggatgcctg ccgcggaagg cgaggccggc cccgagcccg
    18901 cctaccgatc cgccctgcgc cgggtcgcca gctacaacgt cgtgaccgat cccttcgcca
    18961 cgctgatggc cgagcaggac gccgaattgc gagccgcgac cgagctcagc cgcggcatcc
    19021 gcaccctttt gaccctgcac ttccagggcg gtccggcgtg aaggtgcaga ccgggtctgc
    19081 cgacggtttc tgccggcggc ccgacccggc gatcggcgtg gtcctgctct acggtcccga
    19141 ccagggcctc gtcgccgatc gtcggcgcca gttggtcgag gcggtgctgg gggccgcgga
    19201 cgaccccttc cggttgaccg agctcgaggc cgagcgggtc acggcagccc ccgggctcct
    19261 gctcgaggag gcgcaggcgc tctcgctggt cggcggccgg cgcatcgtgc tgctccgcca
    19321 ggcgaccgac accatggcca agccgctcgc cggcctgctc gggcagggcg gccacgcggc
    19381 gctggtcatc gtcgaggccg gcgagctggg cgccggctcc gccctgcgcc ggctcgccga
    19441 gaaggatcgg gcggccgccg cgatcgcctg ctaccggatc gatggcgccg ggctggcgag
    19501 ccagatccgc gaccatctgc gcgggctcgg cctgagcgcc gagccggagg ccatggccta
    19561 cctggtcgag catctgggcg gcaatcggga gctgacccgg gccgagctcg agaagctggc
    19621 gctctacaag ggcgaggacc gagcggcgcg gatcaccctc gacgatgtgg cggcggtggt
    19681 cggcaacagc tctgccctct atgtcgatgc gctggtctgg gcctgtctcg tcggtcggcc
    19741 gggcacggcc gagccaattc tcgatcgcct gctcggcgag ggccaggcgg cggtgcggct
    19801 gattcgcgcg atctcgtcgg ccctgctgcg tctcctgccg ctggcggcgc gggcggccag
    19861 gggcgaggcg ccggcggccg tggtccaggg cgcgcggccg ccggtgcatt tcagccagag
    19921 ggcgtcgatg caacgggcgc tggaaagctg gcgggcggcg ggcctcgagg cggcgctggc
    19981 gcttgccctc gaggccgagc ggcgctgcaa gcgggcgcgc gcgcccgaac ggctgctgct
    20041 gcgtcgcctg gtccacgaga ttgcgctgct gccgcgttga tgccggtcgg tttacggctg
    20101 gccgacgcgg cagcaggcgc taaactccgg ccatcggggg ggagagggcg tgcttcgtta
    20161 cgtgctgcat cggttgatcg tgatgatccc gacggtgatc gccatcagcg tggtcacctt
    20221 cgtcatcatc cagttgccgc cgggcgattt cctctcggcg cagatcagcg agctgcaggc
    20281 gcagggcgag ggggtccaga ccgagcgcat cgcgtatctg cgcgagctct acgggctcga
    20341 gcggccgctc tggcagcaat atctccagtg ggcctggggg ctcctgcacg gcgatctcgg
    20401 ctggtccttc gagtatgacc ggccggtgac ggaggtcatc ggcgacaaga tcttcctcac
    20461 cgtggtcatt tcgctcgcga cagtgatctt cacctggatc gtcgccttcc cgatcgccct
    20521 ctactcggcc acccacaagt acagttgggg cgaccacggg ctgaccttcc tgggctttct
    20581 cggcctcgcc acgccgaact tcctcctcgc actggtcatg ctctatttcg ccaatgtctg
    20641 gttcggcacg tcgatcggcg ggctcatgga tccggtctat ctcgatcagc cgatgagctg
    20701 gggcaagttc gtctccgtcc tgcagcatct gtggatcccg gtgatcatca tcggcaccag
    20761 cggcaccgcc ggcatgatcc gccgcctgcg cgccaatctc atggacgagc tgcagaagcc
    20821 ctatgtggtc accgccaagg ccaagggcct gccgccgggg cgcgcgctgg tcaagtatcc
    20881 cctgcgaatg tcgctcaacc ccttcatcgc cgacatcggc aacctcctgc cggacctgat
    20941 ctcgggctcg gcgctggtcg ccgtcgtgct cagcctgccg accaccgggc cggtcctggt
    21001 ccgtgccctg cagagccagg acatgtacct cgccggcagc tttttgatgc tcgaggcctt
    21061 cctcgtggtg atcggtgtcc tgatctccga tctcctgctc gcctggctcg acccgcgcat
    21121 ccgcttcagc ggcgggcagg cccgctgatg
//
LOCUS       DAOTFI010000060        21086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3005_length_21086_cov_7.428843, whole genome shotgun sequence.
ACCESSION   DAOTFI010000060 DAOTFI010000000
VERSION     DAOTFI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21086
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3005_length_21086_cov_7.428843"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1084
                     /locus_tag="P5558_07960"
     CDS             146..1084
                     /locus_tag="P5558_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRY24281.1"
                     /translation="MTLATALLAGTFAVGPAAAQDKPVLAFVTNAAADFWTIGRAGVM
                     QAGEELDGYTTEMHIVSEATAAEQRRIIDDLLTRGVAGITVSVIDPANSRDLLNRAAS
                     QAVLFTSDSDAADTDRMLYVGTDNVAAGEQAGELIKQALPDGGKIMLFVGTMGAANAQ
                     ERVEGIKKVLEGGNIEIVDIRTDEVDFARAKRNVEDTLTTYPDIDGLVGLWSYNTPQI
                     VEAVKAAGKDGQVKIIGFDEDPVTLRGIADGIVEATVVQQPYEFGYQSMMLMARVLDG
                     DTSFIPEDGLMIIPTKVIDKSNVDEFRQEMQKLLAQ"
     gene            1093..2631
                     /locus_tag="P5558_07965"
     CDS             1093..2631
                     /locus_tag="P5558_07965"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY24282.1"
                     /translation="MTSERGGPPAAPFLRLVRIGKTYPGVTALADVDLEVARGEVVGL
                     IGENGAGKSTLMKVLGGVVAPDSGHIEIDGTVHRALTVPQSLAAGIAFVHQELNLFEN
                     LDVAANIFIGREPRWGGPLHLIDRQLLYRRTRPLLERLKVDFDARTPVATLSIAQRQM
                     VEIAKALSLDARLIIMDEPTSSLTVAETERLLDVIAELQAQGVAVIYITHRLNEVKDC
                     ADRVVCLRDGVVAGALAHDEIAHAAMIRLMIGRDLKALYTPPGRQPATGLEVQGLRTV
                     LFPDRAVDLEIRRGEILGLAGLIGSGRTELARALFGIDPPLAGAIRLDGEPITVRTPH
                     HAIMRGIYLVPEDRKRSGVIVEFPVRCNVTLADLHDHSRAWLISGGRECATTERQKER
                     LHIRTPSIESEVITLSGGNQQKVVLAKWLAMRPSVVIFDEPTRGIDVGAKSEIYAILR
                     QLADAGVAILMISSDMEEVIGVSDRIAVMHEGRISGILENRDSFTETNVLELAVNSPA
                     RTAA"
     gene            2650..3651
                     /locus_tag="P5558_07970"
     CDS             2650..3651
                     /locus_tag="P5558_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24283.1"
                     /translation="MQKKDLGLLVLILVVGAITTALNPVFLSPVNLGNMANLVGLFGI
                     FSIGIGVVIITGGIDLSVGSMFALIGVVFIDLMTNKGMAWELVLPLAIGLGICFGVIH
                     GVLVTRFNMQPFVVTLCGLLIYRGAARYYMADATIGFGYAGGHETLEWLASGRSLGVP
                     HTFIAFVAVAIVMGIVLHRSVFGRYLFAVGKNEEAARYSGIPTNLVIASAYVISGALA
                     GLAAVFLAFYTYSIQPSAHGNFYELYGIAAAVLGGCSLRGGEGSIMGIALGTVLLQVL
                     QNLVNLLGIPTSLNFAVMGTVILLGVLTDQQLQRRRARLLAVKAHQAKTLGAQPARA"
     gene            3818..5374
                     /locus_tag="P5558_07975"
     CDS             3818..5374
                     /locus_tag="P5558_07975"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRY24284.1"
                     /translation="MQLLQVTIPVLALLVAGILAVWAWRAERDTAEQIVLRNARLAAA
                     YSERVLTSLDQLLVQAEKLASPERLRLDRKGLAAELATLVETGEHAISVSIVTPDGQL
                     LLTDRWAGPTLDVSDRPYFQALRDGDETLYIDRLTVQPAGEDAIVVARRRAGEGFAGL
                     VVTGARVAAFADFFRTMASPDDAAVSLIRRDGRLLVRQDTAYPPLTIESPAAVLDAIA
                     GTAPPLYEATAQTDGITRLYATVPVQGFPLFASFGLPTRAIWVAWGWRMLVIVASLAA
                     AVAIALLVLGELSRRLQHALDAAEVARSRAAADRQAMLYQELSHRVKNNLQLVESLLR
                     LRGRGQTGDSRDSLDEVALRVNAIAEVHRQLDSASGDGTVDIARLLRRLTTNPGIVPP
                     ERDIEVSCSTDALELAPDRAIPLALITVEALTNAVKHAFPGDRSGTDSRIDVTLRTDG
                     ITAALSISDNGVGSNGSPRTDQGLGLRLLAALASQLGGALDQRFDGGTTLSVTFPVQP
                     GPQAPEAPAA"
     gene            complement(5395..5877)
                     /locus_tag="P5558_07980"
     CDS             complement(5395..5877)
                     /locus_tag="P5558_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRY24285.1"
                     /translation="MKDLSQLFQHMLMDVYYAEKKILKSLPKMAEKAGDKALVEAFNA
                     HFTETEGQIDRLERVFEMLDRKPEGETCEALDGLVAEAEEIMKEAGSDAVRDAGLLAA
                     AQAVEHYEISRYGTLVAWAQELGLAEVVGLLQETLDQEYAADELLDRLASEKINKLAA
                     "
     gene            complement(6394..6948)
                     /locus_tag="P5558_07985"
     CDS             complement(6394..6948)
                     /locus_tag="P5558_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY24286.1"
                     /translation="MSSLYRTALDELGGVFDRLDDARVDRAVEEIAAARRVVVFGGGR
                     EKLQIMGFAMRLFHMGLAVAVEGDMTTPPVGAGDLFLVTCGPGEISTALALIDVARAA
                     GARVMLITAQPEGRAAARADFVLHLPAQTMADDQGPQRSSVLPMGSLYEGALFVLFEV
                     MVLKLIERLEVTPEAMRANHTNLE"
     gene            complement(6995..8503)
                     /locus_tag="P5558_07990"
     CDS             complement(6995..8503)
                     /locus_tag="P5558_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY24287.1"
                     /translation="MEALLAAFAAALAPQVLLVVVAASVYGLFIGAVPGLTATMGTAL
                     LVPLTFFLEPLAAIGAIVAASTMAIFAGDIPGALLRIPGTPASAAYTDDAYRLTQKGQ
                     AGLALGTNLVCSVLGGLIGVAILVLAAPRIARFSLGFSSYEYFWLALLGLSCATAVSR
                     GSAVKGGLSICAGLLISMIGIDIVTGTPRFTFGDTPLIAGVSIIPVLIGMFAITEILR
                     KCATPAPASRPEALRLGALFAGVGGTVWRYKGGVARSSVLGAFIGALPGAGADIAAWI
                     SYAVAKRFSREPEKFGTGHAEGIVSASSSNNAALSGAYVPALVFGIPGDTITAILIGV
                     LMMKGVTPGPMVFVMDQGLVGAIFAIFVVANLLLLPLGTVAILLSQRLLAVPANVLFP
                     IILLFCAIGAFATNNAYFDIWIMLAVGLLAFALEENGYPLAPMILALILGPQIEESFM
                     QSIIKADGHLLAFFSRPIAGTLGVLTLAIWLAVALLALRSGRRAAGAARRAG"
     gene            complement(8538..9005)
                     /locus_tag="P5558_07995"
     CDS             complement(8538..9005)
                     /locus_tag="P5558_07995"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY24288.1"
                     /translation="MRWNDAVLGAIFVIIALVVALDARGFPDLPGQAYGSGTLPTLVA
                     AVMFCLGAAQIVTGVRSRAPAIRLDAWMRAPGAAWRMAAVPLFVAAFVLLLRPLGFPL
                     VTPPLLFALLLVMTVRPATALVVAVAATAGIWFVFARLLGVPLPLGLLTEIVY"
     gene            complement(9049..10008)
                     /locus_tag="P5558_08000"
     CDS             complement(9049..10008)
                     /locus_tag="P5558_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY24289.1"
                     /translation="MSRLLAAAAIMAAFAGSANAAYPERPLTLIVPWAAGGGTDAIGR
                     MIAQGLQEELGQPVNVVNRTGAGGIVGHKEMVDAEPDGYTIGFATAELGTYRAMGTSE
                     ISPADVTPIALMNFDAAAFSVRADSEWATLADALAAIKAAPAGTYKASGFPVGAGYHL
                     ALSGLLTDNDIDPKAIAVVPSQGAAPGFQELVSGGVEIVPSSLPEAAPMLESNLVRTL
                     AVLAPERLPAYPDVPTAIEAVGTQSVGGSWRGVVGPADLPPEIAERLEAALQAVAESE
                     AYISFMDGRSFGRQWLPAAEFAEFMATAEESATASIEKLGLGQ"
     gene            complement(10150..11391)
                     /locus_tag="P5558_08005"
     CDS             complement(10150..11391)
                     /locus_tag="P5558_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY24290.1"
                     /translation="MDDDAFFGLSRYHFLLAGLGAAIILAHWLPRFFSKREPAAAPLM
                     ILLGMAIALAVPELALLPDPRSSPGPWELISEMAVIVALFASGMRIDRLRPWRQWSPT
                     LRLLALAMPLTILAVALLGVGLMGMTVAGAILLGAVLAPTDPVLAADVQVGPPHEGRE
                     HPVRFALTTEAALNDGLAFPFVHLGLVVAALGANPAIWLWEWLLVDVAYRIAAGVVVG
                     IACGWALGQVLFVLPRAAVLADTASGVVALAGVMLCYGVAELIEGYGFIAVAVMGLTL
                     RRVQVEHFFHRRLHDFGEAIEHALTAILLVALGSVMPLLLGDLTWLHLVVALTLLFVA
                     RPLVTWLALLHTSLTHGSRVRVAVYGVRGIGSIYYLCYAGSHMEFVNEAAIWSLVALV
                     ILLSTMLHGFTVGWAMEGIRD"
     gene            complement(11504..12406)
                     /locus_tag="P5558_08010"
     CDS             complement(11504..12406)
                     /locus_tag="P5558_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY24291.1"
                     /translation="MLDARTNLPPKGLVERAAPTLFLIFWSSGFVAAKAGLEGAAPLT
                     FLSLRFSIVTALMLAIALVLRAPWPSTRAELRHLVVLGLVIQAIYFGCSYLAFGAGIT
                     AGGLALIVGMQPVLTAVVAVPLLGERFRPVQLLGLALGVAGTALVLHEKLAAGVGTVA
                     GVVISLMALVAITGGTLYQKRFCPRFDLWTGGTIQFATAAIVVSLAALLFEDNTVTWT
                     PAFAAGLGYLVLVSSIVSIALLNLMLRRGEASRVATLFFLVPPGAALVAWLLLGESFG
                     PLALVGMAVATTGVALVMRAPRRR"
     gene            12603..14540
                     /locus_tag="P5558_08015"
     CDS             12603..14540
                     /locus_tag="P5558_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24292.1"
                     /translation="MRRLQDFWGLLAAYWVSDRWIEAWALTAAVLALTTALSKASVWV
                     ATASGDFIGALAGYHSPDIGVDPAALLLTSTLMFFALHVGRAGGVACRHLVSTTLHRR
                     ARAWLVGRFDAAILADERIAFDLMSDRADDGTVPARLPDAIDQRLDDCSAGLYAGLIG
                     LAMGFWGAVTSVWFVAAALIERSVSVPLLDDWLASLGLPVDLAPGAYGSALLAGLLIL
                     LYVPAVTMAAWLIGRVLERITLERQKRDGAWRGELGAMLHRVTQLATSRGERAQRAIN
                     TRLYAGVDRAWARQNAWGAGMMLFNDVHGFLARRLIAYLPALPAFTSDAIGFRDFVAS
                     SELTAELIQDVSWFINVMPAIATLRANAGRLTDLARAVERVRDRDAFYAETGISRFRR
                     VHPSAEAGLRLEGIALRHRGHLAQPFLSVPLLRLEPGQWACLRGSNGCGKSSLLKAVA
                     GLWPYGEGEIATGPGQHLLFAGQEPDLPDRLTLKALVAYPEAAETFDDLAVAAALGEA
                     GLGAFIRALHDELHEGHPWRHVLSGGQKQRLVLARILLHRPDVLLLDEATSALDAAAA
                     IGFHESLRDALPRTMVLAVLHGEGLPTDPAGQPFYDTIVRIEAGLARVEPVRRPVPRQ
                     ARVAVLPQAPVASRAAARALS"
     gene            complement(14495..15244)
                     /locus_tag="P5558_08020"
     CDS             complement(14495..15244)
                     /locus_tag="P5558_08020"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY24293.1"
                     /translation="MTAAAPSPTLHHAPARRGDRPIPCHVLAPAGAVTGLVLLLHGRD
                     GAGRSAHIVPIAEAYLARGWRVVAPDLPHSRAVPGSGPVELYTMSGHLEDAAAVLQWA
                     RDAWPEDAERLALAGHSVGAWAAARLAAGTEALDHLLGVSPVFSGPALLAARRAMGAP
                     AVQALRREAPAMLAELPTIQAATALRRVEAPVAVVTGALDGITPPHVARAWFRAAAGA
                     CFFAALPGQHHCLEGPLYDRALAAALDATGA"
     gene            complement(15252..16205)
                     /gene="thiL"
                     /locus_tag="P5558_08025"
     CDS             complement(15252..16205)
                     /gene="thiL"
                     /locus_tag="P5558_08025"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRY24294.1"
                     /translation="MGEFDVIDRLLKPLAKGFPGALGLADDAALVDVPDGRSLVIAKD
                     AIVEGVHFLADDPPEAVAAKLLRVNLSDLAAMGATPLAYLTVIARPKSLDDAWLEGFV
                     AGLAADQKNFGLHLIGGDLVSTPGPLLLSCTILGLVPEGRALTRKGARAGDHIWVSGT
                     LGDAALGLRVLKGLAATEDEAFYLVERYRRPQPRTALGPRLVGLAHAAIDVSDGLVAD
                     LTHILDESAAAATVDADALPLSPVAHGMPGCHDAALVGGDDYELLFTAPDAATPAIEA
                     LAGELGVALTRIGRIAAGQGLTVTDGEGRKIALDGLGWRHF"
     gene            complement(16223..16729)
                     /gene="nusB"
                     /locus_tag="P5558_08030"
     CDS             complement(16223..16729)
                     /gene="nusB"
                     /locus_tag="P5558_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRY24295.1"
                     /translation="MSEAPLRPSAVKPVLNKRRAARFAAVQALYQADAGDVAIDRVVA
                     EFQAHRLAEIFEPLELDEPSPAVDGKWFAILAGGAWDARERLDPAIESCLASGWTLAR
                     CGFLLRACLRAGAFELAERTDVPVRVVLNEYVELAHLFFSGDEPAFINAVLDRLAPIL
                     RPQQSPVT"
     gene            complement(16726..17199)
                     /gene="ribH"
                     /locus_tag="P5558_08035"
     CDS             complement(16726..17199)
                     /gene="ribH"
                     /locus_tag="P5558_08035"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRY24296.1"
                     /translation="MAGISATSAVPRLPGARILVVEARYHAAINDFLLEGARAVLEAA
                     GARFEELTVPGALEIPPAIRIADIHGRGFAGYVALGCVIRGETTHYELVAGESARGIT
                     DLGTAHGLAIGNGILTVENEAQAIERADPARQDKGGHAALAALTLVAFRRHMLQR"
     gene            complement(17199..18320)
                     /gene="ribB"
                     /locus_tag="P5558_08040"
     CDS             complement(17199..18320)
                     /gene="ribB"
                     /locus_tag="P5558_08040"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRY24297.1"
                     /translation="MSDADFLSPIEDVLEDARRGKMFILVDDENRENEGDLVIPAEFC
                     DAAAINFMAKHGRGLICLALSRQRCEKLGLPLMAQGNEPRLSTAFTISIEARDGVTTG
                     ISAADRAHTVKVAIDPESRRDDLVAPGHIFPLMARDGGVLVRTGHTEAAVDLSRLAGL
                     DPSGVICEIMNDDGTMARLPDLVAFAQLHGLKVGKIADLIAYRLKHDRLVERDIATTF
                     TSRFGGDFRLQVYVNVLDGIEHVALVKGDVTAPEPVLVRVHAVNMLQDLLGEGESGRG
                     DQLHQAMRMIGEAGRGVVVLLREPSPTSISERVKVALGHHPQPPADLRVYGIGAQILG
                     DLGVTDMILLSNTGRTIVGLEGYNLRVVETRPIPAEGAR"
     gene            complement(18317..18928)
                     /locus_tag="P5558_08045"
     CDS             complement(18317..18928)
                     /locus_tag="P5558_08045"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRY24298.1"
                     /translation="MFTGIITHQGMILEVENKDGGRRLVVRPDGLDLGRIAIGASVAH
                     SGVCLTVAGKTVESYSVYASPETLARTTLGDWQQGDTINLEASLRLGDELGGHLVFGH
                     VDGLGRIAAIDPSGESFRLAFDMPEDLRPLVAVKGSIAVDGVSLTVNEVTDTGFAVMV
                     IPHTWDVTTLRHRRVGDRVNLEADMLARYVARQLEFVPGGDRS"
     gene            complement(18944..19978)
                     /gene="ribD"
                     /locus_tag="P5558_08050"
     CDS             complement(18944..19978)
                     /gene="ribD"
                     /locus_tag="P5558_08050"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRY24299.1"
                     /translation="MTDPIDARFMGLALALGERHLGLTAPNPSVGCVIVRDGLVVGRG
                     WTQPGGRPHAEAEALARAGDAARGATAYVTLEPCSNWGKTPPCTKGLIEAGIARVVVG
                     CVDPDPRVDGKGIAGLREAGLEVVVGVREAEALAQNLGLYRRIRAGRPMVGLKLAMSL
                     DGRIATRTGDSQWITGEAARAFGHRLRASHDAIMIGSGTALADDPLLTCRLPGLEGRS
                     PVRVVMDRRGRLPATSQLRASADRLPLVHVTAADGDTSPGPVLTRLADQGITRLLVEG
                     GASLATSLLEAGLVDRLYVFSAPALIGGDGLAAIQGLGVDRMADVGRWRVVDTRLLGR
                     DRFSVLEPEG"
     gene            complement(19989..20456)
                     /gene="nrdR"
                     /locus_tag="P5558_08055"
     CDS             complement(19989..20456)
                     /gene="nrdR"
                     /locus_tag="P5558_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRY24300.1"
                     /translation="MRCPFCGSEDTQVKDSRPVEDATSIRRRRQCTACGARFTTFERV
                     QLRDLVVLKKDNKRVPFDRDKLARSIAIAVRKRGVDQEQIERIVNGIVRRIETSGEHE
                     VPTTRIGELAMESLAKLDEVAYVRFASVYRDFANARDFEEFISRLPEADEAEP"
     gene            complement(20559..20849)
                     /locus_tag="P5558_08060"
     CDS             complement(20559..20849)
                     /locus_tag="P5558_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24301.1"
                     /translation="MDTWPDDDYLDLVQPAHLTFKGASNGEIAFGALEGQLDVRYGAR
                     DGAACAEFTWDGQDEGDPVCGRGWVVLGSAGRLVGHIYIHRGEESGFVCEPD"
BASE COUNT         3312 a         7477 c         7361 g         2936 t
ORIGIN      
        1 ctgaggcgcc cgggcgcgcg acgggtgcgc cccagaagcc gattgacctc gcccggccgc
       61 cgaagcacca tccatcgcgt cgctccagcg gggcgaccga acgatcagga attcaggggg
      121 aaacgagatg ctacgacgtc acgccttgac gctcgcgacc gcgctgctcg ccggcacgtt
      181 cgccgtcggc ccggcagccg cccaggacaa gccggtcctc gccttcgtca ccaacgccgc
      241 cgccgacttc tggaccatcg gccgcgccgg cgtcatgcag gccggcgagg agctcgacgg
      301 ctacaccacc gagatgcaca tcgtctccga ggccaccgcc gccgagcagc gccgcatcat
      361 cgacgacctc ctcacccgcg gcgtcgccgg catcaccgtc agcgtgatcg acccggccaa
      421 cagccgcgac ctcctcaacc gcgccgcctc gcaggccgtg ctcttcacct cggacagcga
      481 cgccgccgac accgaccgca tgctctatgt cggcaccgac aacgtcgccg cgggcgagca
      541 ggcgggcgag ctgatcaagc aggcgctgcc ggacggcggc aagatcatgc tcttcgtcgg
      601 caccatgggt gccgccaacg cccaggagcg cgtcgagggc atcaagaagg tgctcgaggg
      661 cggcaatatc gagatcgtcg acatccgcac cgacgaggtc gacttcgccc gcgccaagcg
      721 caatgtcgag gacacgctga cgacctaccc ggacatcgac ggcctcgtcg gcctctggtc
      781 ctacaacacg ccgcagatcg tcgaggcggt gaaggccgcc ggcaaggacg gccaggtcaa
      841 gatcatcggc ttcgacgagg atccggtgac cctgcgcggc atcgccgacg gcatcgtcga
      901 ggcgaccgtc gtgcagcagc cctacgagtt cggctaccag tcgatgatgc tgatggccag
      961 ggtgctcgac ggcgacacct cgttcatccc cgaggacggg ctgatgatca tcccgaccaa
     1021 ggtgatcgac aagtcgaacg tcgacgagtt ccgccaggag atgcagaagc tcctggccca
     1081 gtgagcgatg cggtgaccag cgagcggggc ggccctccgg ccgccccctt cctccggctc
     1141 gtccggatcg gcaagaccta tccgggcgtg accgccctcg ccgatgtcga tctcgaggtc
     1201 gccaggggcg aggtggtcgg gctgatcggc gagaacggcg ccggcaagtc gaccctgatg
     1261 aaggtgcttg gtggcgtcgt cgcccccgac agcgggcata tcgagatcga cggcaccgtt
     1321 caccgggcgc tgaccgtccc gcagtcgctg gcggcgggca tcgccttcgt ccaccaggag
     1381 ctgaacctct tcgagaatct cgacgtcgcc gccaacatct tcatcggccg cgagccgcgc
     1441 tggggcgggc cgctgcacct gatcgaccgc cagctcctct accggcggac gcgccccctg
     1501 ctcgagcgcc tcaaggtcga tttcgatgcc cgcacacccg tggccacgct ttccatcgcc
     1561 cagcggcaga tggtggagat cgccaaggcg ctctcgctcg acgcccggct gatcatcatg
     1621 gacgagccga cctcgagcct cacggtcgcc gagacggagc ggctgctcga cgtcatcgcc
     1681 gagctccagg cgcagggtgt cgccgtcatc tacatcaccc accggctcaa cgaggtgaag
     1741 gactgcgccg accgcgtcgt ctgcctgcgc gacggcgtcg tcgccggcgc gctcgcccat
     1801 gacgagatcg cccatgcggc gatgatccgc ctgatgatcg gccgcgacct caaggccctc
     1861 tacacgccgc cgggccgcca gccggccacc gggctcgaag tgcagggcct gcgcaccgtc
     1921 ctctttccgg accgtgcggt cgacctcgag atccgccggg gcgagatcct gggcctcgcc
     1981 gggctgatcg gctcgggccg caccgagctc gcccgggccc tcttcggcat cgacccgccc
     2041 ttggccggcg ccatccggct cgacggcgag cccatcaccg tgcgcacacc gcaccacgcg
     2101 atcatgcggg gcatctatct cgtgcccgag gaccgcaagc gctcgggcgt gatcgtcgag
     2161 ttcccggtgc gctgcaacgt caccctcgcc gatctccatg accacagccg ggcctggctg
     2221 atcagcggcg gccgggagtg tgccaccacc gagcggcaga aggagcggct ccatatcagg
     2281 acaccctcca tcgagagcga ggtgatcacc ctctcgggcg gcaaccagca gaaggtggtg
     2341 ctcgccaaat ggctggcgat gcgcccctcg gtcgtcatct tcgacgagcc gacccgcggc
     2401 atcgacgtcg gcgcgaagag cgagatctac gccatcctgc gccagctcgc cgatgccggc
     2461 gtcgccattc tgatgatctc cagcgacatg gaggaggtga tcggcgtcag cgatcgcatc
     2521 gccgtcatgc acgagggccg gatcagcggc atcctcgaga accgcgacag cttcaccgaa
     2581 accaacgtgc tcgagctcgc cgtcaactcg ccggcccgca ctgccgcata acccggggcg
     2641 accgccgcca tgcagaagaa agacctgggc ctcctcgtgc tgatcctcgt cgtgggcgcc
     2701 atcaccacgg cgctcaaccc cgtcttcctg tcgccggtca atctcggcaa catggcgaac
     2761 ctggtcggcc tcttcggcat cttctcgatc ggcatcggcg tcgtcatcat caccggcggc
     2821 atcgacctct ccgtcggctc gatgttcgcg ctgatcggcg tcgtcttcat cgacctcatg
     2881 accaacaagg gcatggcctg ggagctggtc ctgccgctcg ccatcgggct cggcatctgc
     2941 ttcggcgtca tccacggtgt gctggtgacc cgcttcaaca tgcagccctt cgtcgtcacc
     3001 ctctgcggcc tcctgatcta ccgcggcgcc gcccgctact acatggccga cgccaccatc
     3061 ggcttcggct acgccggtgg ccacgaaacg ctggaatggc tggcctcggg ccgcagcctc
     3121 ggcgtgccgc acaccttcat cgccttcgtc gccgtcgcca tcgtcatggg catcgtgctg
     3181 caccgctcgg tcttcggccg ctacctcttc gcggtcggca agaacgagga ggcggcacgc
     3241 tactccggca tcccgaccaa cctcgtcatc gcctcggcct atgtgatcag cggcgccctc
     3301 gccggcctcg cggcggtctt cctcgccttc tacacctact ccatccagcc ctcggcgcac
     3361 ggcaatttct acgagctcta cggcatcgcc gcggcggtgc tcggcggctg ctcgctcagg
     3421 ggcggcgagg gctcgatcat gggcatcgcc ctcggcacgg tgctgctgca ggtcctgcag
     3481 aacctcgtca acctcctggg catcccgacc tcgctcaatt tcgcggtcat gggcaccgtc
     3541 atcctgctcg gcgtgctcac cgaccagcag ctccagcggc gccgcgcccg gctgctcgcc
     3601 gtcaaggcgc atcaggcgaa gaccctgggc gcccagccgg cccgcgcctg acccggaccc
     3661 cgcggagggg cgagcccatc ccaccccaac aattgttaat gacaaattcc cggcggcatg
     3721 gcgaactccg gccggcgtat tccggcgcag cgatcggcag gggcaggaaa ccggcaggat
     3781 gtggcgcagt ttgtcgtcga ggttgcgcac accccgcttg cagcttctgc aggtcaccat
     3841 cccggtcctg gcgctgctgg tcgccggcat cctggcggtc tgggcctggc gggccgagcg
     3901 cgacaccgcc gagcagatcg tgctgcgcaa cgccaggctg gcggcggcat acagcgagcg
     3961 cgtgctgacc agcctcgatc agctcctcgt ccaggccgaa aagctggcct cgcccgagcg
     4021 cctgcgcctc gatcgcaagg gtctggcggc ggagctcgcc accctggtcg agaccggcga
     4081 gcacgccatc agcgtgagca tcgtcacgcc ggatggccag ctcctcctga ccgaccgatg
     4141 ggccgggccg accctcgacg tcagcgaccg gccctatttc caggcgctcc gcgacggcga
     4201 cgagacactg tacatcgacc ggctgaccgt gcagccggcc ggcgaggacg cgatcgtcgt
     4261 cgcccgccgc cgcgccggcg aggggttcgc cggcctcgtc gtgaccggcg cccgcgtcgc
     4321 cgccttcgcc gatttcttca ggaccatggc gtcgccggac gacgccgcgg tgtcgctgat
     4381 ccgtcgcgac gggcggctgc tcgtccgtca ggacacggcc tatccgccgc tcacgatcga
     4441 gtcgccggcc gcggtcctgg acgcgatcgc cggtaccgcc ccgccgctct acgaggcgac
     4501 ggcgcaaacc gacggcatca cccgcctcta tgcgaccgtg ccggtccagg gctttccgct
     4561 cttcgccagc ttcggcctgc cgacccgcgc gatctgggtc gcctggggct ggcggatgct
     4621 ggtgatcgtc gccagcctcg cggcagccgt ggcgatcgcc ctcctggtcc tgggcgagct
     4681 gagccggcgc ttgcagcacg cgctagacgc cgccgaggtc gcgcggagcc gcgcggcggc
     4741 cgaccggcag gccatgctct accaggagct cagccaccgg gtgaagaaca acctgcagct
     4801 cgtcgaatcg ctgctgcgcc tgcgtggccg cggccagacc ggcgattcgc gcgacagcct
     4861 cgacgaggtg gcgctgcgcg tcaacgccat cgccgaggtc caccgccagc tcgacagcgc
     4921 gtccggcgac gggacggtcg atatcgcccg cctgctgcgg cgcctgacga cgaacccggg
     4981 catcgtgccg cccgagaggg acatcgaggt ttcgtgcagc accgatgcgc tggagctggc
     5041 accggatcgc gcgataccgc tggccctgat cacggtcgaa gccctcacca acgcggtcaa
     5101 gcatgccttt ccgggcgatc ggtcgggcac cgattcccgg atcgacgtca cgctgcgcac
     5161 cgatggcatc accgccgccc tctcgatctc cgacaacggc gtcggctcga atggcagccc
     5221 ccggaccgac cagggcctcg gcctgcgcct cctcgcggcc ctcgccagcc agctcggagg
     5281 cgctctcgat cagcgcttcg acggcggcac cacgctcagc gtcaccttcc ccgtgcagcc
     5341 cggccctcag gcgcccgagg caccggcggc ctgagccgcc ggcgccgccc gcgctcaggc
     5401 agcgagcttg ttgatcttct cggacgccag ccggtcgagc agctcgtcgg ccgcatactc
     5461 ctggtcgagg gtctcctgca gcagccccac cacctcggcg agcccgagct cctgggccca
     5521 ggcgacgagc gtgccgtagc gcgagatctc gtagtgctcg accgcctgcg ccgcagccag
     5581 caggccggca tcgcgcaccg cgtccgaccc ggcctccttc atgatctcct cggcctcggc
     5641 gaccagcccg tcgagcgcct cgcaggtttc cccctcgggc ttcctgtcga gcatctcgaa
     5701 cacccgctcg agtcggtcga tctgcccctc ggtctcggtg aaatgcgcgt tgaacgcctc
     5761 gacgagcgcc ttgtccccgg ccttctccgc catcttcggc aggctcttga ggatcttctt
     5821 ctcggcgtaa tagacgtcca tcagcatgtg ctggaaaagc tggctcaggt ccttcacggt
     5881 cgttcctttc ccgggggttg cgcgggccgc gctcagcccg cagcaccgcc cgccaagaac
     5941 cggcgagccg ccaccgagtt ccgcgccgtc gggcgtcggc gcgccggccc gggacccggc
     6001 cggccactgt cgcaaaatcc gcgacaccac cgctcgggtt gtcgcggatt ccgcgacaga
     6061 attgcttggg ttgtcgcgga ttccgcgaca ggcgaacccg ggatgtcgca gattccgcga
     6121 cagttttccc gggatgtcgc ggattccgcg acatcccgcg acgaacgaac ccgccgaacc
     6181 gacgagcgaa cccacggaat cgacgatcga acccggcgat tcgacgatcg aacccgggct
     6241 ttcgacgaac gaacccaaag gcccgacccg gcgattgcgg gcatccgcct gccttcaggc
     6301 cggcgaagga acccgaaagg caggatttgc cgaaggaacc cgcgcccctt gactcggccc
     6361 acgagaaagg ctattattcc tattgtgggc ggcctattcc aggttggtgt ggttggcccg
     6421 catggcctcc ggcgtgacct cgagccgctc gatcagcttc aggaccatca cctcgaagag
     6481 cacgaagagt gcgccctcgt agagcgagcc catcggcagg accgagctgc gctgcggccc
     6541 ctggtcgtcg gccatggtct gggccggcag atgcagcacg aaatcggccc gggccgcggc
     6601 ccgcccttcg ggctgggccg tgatcagcat caccctggcc cccgccgccc tcgccacgtc
     6661 gatcagcgcc agggcggtcg agatctcgcc gggcccgcag gtgacgagga agaggtcgcc
     6721 cgcccccacc ggcggcgtgg tcatgtcgcc ctcgaccgcc acggcgagcc ccatgtggaa
     6781 gagccgcatg gcgaagccca tgatctgcag cttctcccgg ccgccgccga agacgacgac
     6841 ccgccgggcg gcggcgatct cctcgaccgc ccggtcgacc cgggcgtcgt ccaggcgatc
     6901 gaagacgccg ccgagctcgt ccagggcggt tcggtacagg ctgctcatgt ggcatgctcc
     6961 tcgatggtgg tccgtgggcc tgcggccggc atcgtcaccc ggctcgccgg gctgctccgg
     7021 ccgcccggcg cccggagcgc agggcgagga gcgccacggc cagccagatc gccagcgtca
     7081 gcacgccgag cgtgccggcg atcggccggc tgaagaacgc gagcaggtgc ccgtccgcct
     7141 tgatgatcga ctgcatgaag ctctcctcga tctgcgggcc gaggatgagc gccaggatca
     7201 tcggcgccag cgggtagccg ttctcctcca gggcgaaggc gaggaggccc accgccagca
     7261 tgatccagat gtcgaaatag gcgttgttgg tggcgaaggc gccgatggcg cagaagagca
     7321 ggatgatcgg gaagagcacg ttcgcgggca cggcgagcag ccgctgcgac aggaggatgg
     7381 caaccgtgcc gagcggcagc agcaggaggt tggcgacgac gaagatggcg aagatggcgc
     7441 cgaccagccc ctggtccatg acgaagacca tgggccccgg cgtcaccccc ttcatcatca
     7501 gcaccccgat caggatggcc gtgatggtgt cgcccgggat gccgaagacg agggccggga
     7561 cataggcgcc ggagagcgcg gcgttgttcg agctcgaggc gctgacgatg ccttccgcgt
     7621 ggcccgtgcc gaacttttcg ggctcgcgcg agaagcgctt ggcgacggca tagctgatcc
     7681 aggcggcgat gtcggcgccg gccccgggca gggccccgat gaaggcgccg aggacggacg
     7741 agcgggcgac gccgcccttg tagcgccaga ccgtgccgcc gacgcccgcg aagagggcac
     7801 cgagccgcaa tgcctcgggc ctcgaggccg gcgccggcgt ggcgcatttc ctcaggatct
     7861 cggtgatcgc gaacatcccg atgaggaccg ggatgatcga caccccggcg atcagcggcg
     7921 tgtcgccgaa ggtgaagcgc ggggtgcccg tgacgatgtc gatgccgatc atcgagatca
     7981 ggaggccggc gcagatcgag agcccgccct tgaccgccga gccccggctc accgcggtgg
     8041 cgcaggagag cccgagcagg gccagccaga aatactcgta gctcgagaag ccgagcgaga
     8101 agcgggcgat gcgcggggcg gcgagcacca ggatggccac gccgatcagc ccgcccagga
     8161 ccgagcagac gaggttggtg ccgagcgcca gcccggcctg gcccttctgc gtcagccggt
     8221 aggcgtcatc ggtgtaggcg gccgaggccg gcgtgccggg gatgcgcagg agggcgcccg
     8281 ggatgtcgcc ggcgaagatc gccatggtcg aggcggcgac gatggcgccg atggcggcga
     8341 gcggctcgag gaagaaagtg agcggcacca ggagcgccgt gcccatggtg gcggtcaggc
     8401 ccggcacggc gccgatgaag aggccgtaga cggatgccgc gaccacgacc agcagcacct
     8461 gcggcgccag ggcggcggcg aaggccgcga gcagcgcttc catcaggaaa cgacctcgac
     8521 ccgctcgccg cacaccatca gtagacgatc tcggtcagga gcccgagcgg cagcggcacg
     8581 ccgagcagcc gcgcgaagac gaaccagatg cccgccgtcg cggcgacggc gacgacgagg
     8641 gcggtcgccg gccggaccgt catcacgagg aggagcgcga agagcaaagg cggcgtgacc
     8701 agcgggaagc ccagcgggcg caggagcagg acgaaagcgg cgacgaaaag cggcacggcc
     8761 gccatgcgcc aggccgcccc cggtgcccgc atccaggcgt cgagccggat cgccggggcg
     8821 cggctcctga cgcctgtgac gatctgcgct gcccccaggc agaacatcac cgccgccacc
     8881 agcgtcggca gggtgccgga gccgtaggcc tgccccggca gatcgggaaa gccgcgcgcg
     8941 tcgagggcga cgacgagggc gatgatcacg aagatcgcac ccagcaccgc gtcgttccat
     9001 ctcatgcgcg cggctccacc gccgccgacg ggcggttgcc cgtttcgcct actggccgag
     9061 cccgagcttc tcgatcgaag ccgtggcgct ctcctcggcg gtcgccatga actcggcgaa
     9121 ctcggccgcc ggcagccact gccggccgaa gctgcggccg tccatgaagg aaatgtaggc
     9181 ctcgctctcg gccacggcct ggagcgctgc ctcgagccgc tcggcaatct ccggcggcag
     9241 gtcggccggg ccgaccacgc cgcgccagga accgccgacc gactgcgtgc cgaccgcctc
     9301 gatcgccgtc ggcacgtcgg gataggcggg caggcgctcg ggcgccagca cggccagcgt
     9361 gcggaccagg ttgctctcca gcatcggcgc cgcttccggc agggacgagg ggacgatctc
     9421 gacgccgccc gagaccagct cctggaagcc cggcgccgca ccctggctcg gcaccacggc
     9481 aatggccttc ggatcgatgt cgttgtcggt caggaggccg gagagagcga ggtgatagcc
     9541 ggcgccgacc gggaagcccg atgccttgta ggtgccggcc ggggccgcct tgatcgccgc
     9601 cagcgcatcg gccagcgtcg cccactcgct gtcggcgcgg acgctgaagg ccgccgcgtc
     9661 gaagttcatg agggcgatcg gcgtgacgtc ggccggcgag atctccgacg tgcccatggc
     9721 gcggtaggtg ccgagctcgg ccgtggcgaa gccgatggtg tagccgtcgg gctccgcatc
     9781 gaccatctcc ttgtggccga caatgccgcc cgccccggtg cggttcacca cgttgaccgg
     9841 ctgccccagc tcctcctgca ggccctgggc gatcatccgc ccgatcgcat ccgtcccgcc
     9901 gccggcggcc cagggcacga tcagcgtgag ggggcgctcc ggataggccg cgttagcgct
     9961 gccggcaaag gccgccatga tcgccgccgc ggcgagcaac ctgctcatgt tcctcattct
    10021 ttcctccctc aggcggttgc cctgtccgcg ttcgcggcag cggctctgat tcgatgcgcc
    10081 tgaaaatgcc cgcgcggcag cccgtcttgc aagctctttg accacggccg gcgcccgcgg
    10141 ctattcttcc tagtctcgga ttccctccat ggcccagccg acggtgaagc cgtgcagcat
    10201 cgtcgagagg aggatgacca gcgccaccaa cgaccagatc gccgcctcgt tcacgaactc
    10261 catgtggctg cccgcgtagc agagatagta gatcgacccg atgccgcgca cgccgtagac
    10321 cgcgacccgc acccgcgaac cgtgggtcag ggaggtgtgc agcagggcga gccaggtgac
    10381 gagcggccgg gcgacgaaga gcagcgtcag tgccacgacg agatgcagcc aggtcaggtc
    10441 gcccagcagc agcggcatga cgctgcccag cgccaccagc aggatcgccg tcagcgcatg
    10501 ctcgatggcc tcgccgaaat cgtgaagccg ccggtggaag aagtgctcga cctggacccg
    10561 gcgaagcgtc agccccatca ccgcgaccgc gatgaacccg tatccctcga tgagctcggc
    10621 aacgccgtag cagagcatca cgccggccag ggcgacgacg ccggaagccg tatcggcgag
    10681 caccgcggcc ctcggcagca cgaagagcac ctggcccagc gcccagccgc aagctatgcc
    10741 gacaacgacg ccggcggcga tgcggtaggc gacgtcgacc aggagccatt cccagagcca
    10801 gatggccgga ttcgcgccca gcgccgcgac gaccagcccg agatggacga agggaaaggc
    10861 gaggccgtcg ttcagcgccg cttcagtcgt cagcgcgaag cgcaccgggt gctccctgcc
    10921 ctcatggggc gggccgacct gaacgtcggc ggcgagcacc gggtcggtcg gggccaggac
    10981 cgcgccgagc aggatggccc ccgccaccgt catgcccatc aggccgaccc ccagcagcgc
    11041 cacggcgagg atggtgagcg gcatggccag cgccagcagc cgcagcgtcg ggctccattg
    11101 ccgccacggc cgaagacgat cgatccgcat gccgctcgcg aacagtgcca cgatgaccgc
    11161 catctcgctg atcagctccc agggcccggg cgatgaacgt gggtccggca gcagggccag
    11221 ctcgggcact gccaaggcga tggccatccc cagcaggatc atcagcggtg ccgccgccgg
    11281 ctcgcgcttc gagaagaagc ggggcagcca gtgagccagg atgatggcag cacccaaccc
    11341 ggccagcagg aaatgatacc gcgagagacc gaagaatgcg tcgtcgtcca tgctctccac
    11401 caatctggcg ccatgcacgt caacggatgg aaccgccaaa cgttcggggc caaggccctg
    11461 catccgcgcc ggcccactgg aaagcggcag ccgccgggcg agctcaccga cgccgcggcg
    11521 cccgcatgac cagcgccacg ccggtcgtgg cgacggccat gccaaccagc gccagcgggc
    11581 cgaagctctc gcccagcagg agccaggcga cgagggcggc ccccggcggg acgaggaaga
    11641 agagggtggc gacgcgactg gcctcgccgc ggcgcagcat gaggttgagc agggcgatgg
    11701 agacgatcga ggagacgagc acgagatagc cgagcccggc ggcgaaggct ggggtccagg
    11761 tgaccgtgtt gtcctcgaag aggagggcgg cgaggctgac gacgatggcg gcggtggcga
    11821 actggatggt gccgccggtc cagaggtcga agcgcgggca gaagcgcttc tggtagagcg
    11881 tgccgccggt gatggcgacc agcgccatga gcgagatgac gacgccggcg acggtgccga
    11941 cgccggcagc gagcttctcg tgcagcacga gggcggtgcc ggcgacgccc agagcgaggc
    12001 cgaggagctg caccgggcgg aaacgctcgc cgagcagcgg gacggcgacg acggcggtga
    12061 gcacgggctg catgccgaca atgagcgcga gaccgcccgc ggtgatgccg gcgccgaagg
    12121 cgaggtagga gcagccgaaa tagatcgcct ggatgaccag gccgaggacc acgaggtggc
    12181 ggagctcggc ccgggtgctg ggccagggcg ccctgagcac gagggcgatc gccagcatca
    12241 gcgcggtgac gatggaaaaa cgcaagctga ggaaggtgag cggggcggcc ccctcgagcc
    12301 cggccttggc agcgacgaag ccggacgacc agaagatcag gaagagcgtc ggggcggcgc
    12361 gctccacgag gcccttgggc gggaggttgg tgcgggcatc gagcacggct gcgtctgctt
    12421 tttggatgcg gacggccatc ctcgcccgtg gccattccct accacgcccg ggagcgggcc
    12481 ggatggccac aatgcgacag aatcgaggcc aaagttcggg ttttcgtgga cagcgcgcaa
    12541 tccctcgatt tatacaacta atggtttaat taagctgcgt tcgacagcgt tgtgggcggc
    12601 tgatgcgacg gttgcaggac ttctggggcc tgctcgcggc ttattgggtc tcggaccgat
    12661 ggatcgaggc ctgggcgttg acggcggcgg tgctggcgtt gaccacggcc ttgagcaagg
    12721 cttccgtctg ggtggcgacg gcgtcaggcg acttcatcgg cgcgctcgcc ggctaccaca
    12781 gccccgatat cggcgtcgat cctgccgccc tgctcctgac cagcaccctc atgttcttcg
    12841 cgctccatgt cgggcgcgcc ggcggtgtgg cctgccgcca cctggtctcg acgacgctgc
    12901 accggcgggc gcgggcttgg ctggtcggcc gcttcgacgc cgcgatcctg gcggacgagc
    12961 ggatcgcctt cgacctgatg agcgaccggg ccgatgacgg gacggtgccg gcgcgcctgc
    13021 cggatgccat cgaccagcgc ctcgacgact gctccgccgg cctctatgcg gggctgatcg
    13081 gcctggccat gggcttctgg ggtgccgtca cctcggtctg gttcgtcgcg gcggccctca
    13141 tcgagcgcag cgtcagcgtg ccgctgctcg acgactggct cgcatcgctc ggcctgccgg
    13201 tcgacctcgc ccccggcgcc tacggctccg ccctgctggc cggtctgctc atcctcctct
    13261 acgtcccggc ggtcaccatg gccgcctggc tgatcggccg ggtgctggag cggatcaccc
    13321 tggagcggca gaagcgcgac ggcgcctggc gcggcgagct cggcgccatg ctgcaccggg
    13381 tgacccagct cgccacgtcc aggggcgagc gggcgcagcg ggcgatcaac acccggctct
    13441 atgccggcgt cgaccgggcc tgggcccggc agaacgcctg gggggccggg atgatgctct
    13501 tcaacgacgt ccacggcttc ctggcgcggc ggctcatcgc ctacctgccc gccctgcccg
    13561 ccttcacctc cgacgccatc ggctttcgcg acttcgtcgc cagctccgag ctgacggccg
    13621 agctgatcca ggacgtctcc tggttcatca acgtcatgcc cgcgatcgcc acgctgcgcg
    13681 ccaatgccgg gcggctgacc gacctcgccc gggccgtcga gcgggtgcgg gaccgggacg
    13741 cgttctatgc cgagaccggg atcagccgct ttcgccgggt gcacccatcg gccgaagccg
    13801 gcctccggct cgaggggatc gcgctgcgtc accggggcca tctggcccaa cccttcctca
    13861 gcgttccgct gctccgactc gaacccgggc agtgggcctg cctgcgcggc tcgaacggct
    13921 gcggcaagag ctcgctcctg aaggccgtcg ccgggctctg gccctatggc gagggcgaga
    13981 tcgccacagg cccgggacag cacctcctct tcgccggcca ggagccggac ctgccggacc
    14041 ggctgacgct gaaggcgctg gtcgcctatc cggaggcggc ggagacgttc gacgacctcg
    14101 ccgtcgccgc ggccctcggc gaggccgggc tcggggcgtt catcagagcg ctgcacgacg
    14161 agctgcacga ggggcatccc tggcgccatg tgctctcggg cgggcagaag cagcgcctgg
    14221 tgctggcgcg gatcctcctg caccggccgg acgtgctgct gctggacgag gcgacctcgg
    14281 ccctcgacgc ggcggcggca atcgggttcc acgaatccct gcgcgacgcc ctcccccgga
    14341 cgatggtcct cgccgtgctg catggcgagg gactgccgac cgacccggcc ggtcagccct
    14401 tctatgacac catcgtcagg atcgaggccg gcctcgcccg ggtcgagcca gtccggcgtc
    14461 cggtcccccg acaggcgcgc gtggccgtcc tgcctcaggc gcccgtggcg tcgagggcgg
    14521 cggcgagggc cctgtcgtag agcggtcctt cgaggcagtg atgctggccc ggcagcgccg
    14581 cgaagaagca ggcgccggcg gcggcgcgga accaggcgcg cgcgacgtgc ggcggggtga
    14641 tgccgtcgag cgcaccggtg acgacggcga cgggggcttc gacgcgacgg agtgcagtgg
    14701 ccgcctggat cgtcggaagc tcggccagca tggccggcgc ctcccggcgc agcgcctgca
    14761 ccgccggcgc acccatggcc cgccgggcgg cgaggagggc ggggccggag aagaccggcg
    14821 agacgccgag gagatggtcc agcgcttccg tcccggccgc cagcctcgcg gccgcccagg
    14881 cgccgacgct gtgcccggcg agcgccagcc gttccgcatc ctcgggccag gcatcgcggg
    14941 cccattggag caccgccgcg gcatcctcca gatggccgct catggtgtag agctcgaccg
    15001 gcccggaccc cggcaccgcc cgcgagtgcg gcaggtcggg ggcgacgacc cgccagcccc
    15061 gggcgagata ggcttcggcg atgggcacga tatgcgccga gcgaccggcc ccgtcccgcc
    15121 cgtgcagcag gaggacgagg ccggtcacgg caccggccgg ggcgagcacg tgacagggga
    15181 tcggccggtc gccgcgccgc gccggggcgt ggtgcagcgt cgggctcggg gcggcggcgg
    15241 tcatgaccgg ctcagaaatg ccgccagccg aggccgtcga gggcgatctt ccggccctcg
    15301 ccgtcggtga cggtgaggcc ctggccggcg gcgatccggc cgatccgcgt gagggccacg
    15361 ccgagctcgc cggccaacgc ctcgatggcc ggggttgcag catccggggc ggtgaagagc
    15421 agctcgtagt cgtcgccgcc caccagcgcc gcatcgtggc agcccggcat gccatgggcg
    15481 acgggcgaga gcggcagggc atcggcgtcg acggtggcgg ctgccgccga ttcgtcgagg
    15541 atgtgggtca ggtcggcgac cagcccgtcc gagacgtcga tcgcggcatg cgccagaccg
    15601 acgaggcggg ggccgagggc ggtccggggc tgcgggcgac gatagcgctc gaccagatag
    15661 aaggcctcgt cctcggtggc ggcgaggccc ttgagcacgc gcagcccgag ggcggcgtca
    15721 ccgagcgtgc cggaaaccca gatgtggtcg ccggccctgg cacccttgcg ggtcagggcc
    15781 cgcccctcgg gcacgagacc caggatggtg caggaaagaa gcagcggccc cggtgtcgag
    15841 acgagatcgc cgccgatgag atgcaggcca aagttcttct ggtccgcggc gaggccggca
    15901 acgaagccct cgagccaggc atcgtccagc gatttgggcc gggcgatgac ggtgagatag
    15961 gcgagcggcg tcgcccccat ggcggcgagg tcggaaagat tgacccggag cagcttggcc
    16021 gcgaccgctt ccggcggatc gtcggcgagg aaatgcacgc cctcgacgat ggcatccttg
    16081 gcgatgacga gcgagcggcc gtccggcacg tcgacgaggg cggcatcgtc ggcgaggccg
    16141 agggcgcccg gaaagccttt ggccagcggt ttcagcaacc ggtcgatgac atcgaactcg
    16201 cccattcccg caggcgtcac catcaggtca ccgggctctg ctgggggcga aggatcggtg
    16261 cgaggcgatc gagcaccgcg ttgatgaagg ccggctcgtc gccgctgaag aagagatgcg
    16321 ccagctcgac atactcgttg agcacgaccc tgaccggaac gtcggtgcgc tcggcgagct
    16381 cgaaggcgcc ggcccgaagg caggcgcgca ggagaaagcc gcagcgggcg agggtccagc
    16441 ccgatgccag gcaggactcg atcgccggat cgagccgctc gcgcgcgtcc caggcaccgc
    16501 cggcgaggat ggcgaaccat ttgccgtcga ccgcgggcga gggctcgtcc agctcgagcg
    16561 gctcgaagat ttcggccagc cgatgcgcct ggaactcggc gacgacacgg tcgatggcga
    16621 catcgccggc atcggcctgg tagagcgcct ggacggcggc gaagcgggcg gcgcgccgct
    16681 tgttgaggac gggcttgacg gcggacggcc ggagcggggc ttcactcatc tctgcagcat
    16741 gtggcgccgg aaggcgacga gggtcaacgc cgcaagtgcc gcgtggccgc ccttgtcctg
    16801 gcgcgccgga tcggcgcgct cgatcgcctg cgcctcgttc tcgacggtga ggatgccgtt
    16861 gccgatggcg aggccatggg cagtgccgag atccgtgatg cctcgggcac tctcgccggc
    16921 gaccagctcg taatgcgtgg tctcgccacg aatgacgcag cccagcgcga cgtagccggc
    16981 aaagccccgg ccatggatat ccgcgatgcg aatggcgggc gggatctcga gcgcccccgg
    17041 cacggtcagc tcctcgaacc tggccccggc ggcctcgagc acggcgcgcg caccttcgag
    17101 caggaagtcg ttgatggccg catggtagcg ggcctcgacg acgaggatgc gcgcacccgg
    17161 cagcctcggg acagcactcg tggccgagat gccggccatc aacgcgctcc ctctgcgggg
    17221 atcggccggg tctcgacaac gcgcaaattg tagccctcga ggccgacgat ggtgcggccc
    17281 gtgttggaga ggaggatcat gtcggtgacg ccgagatcgc ccaggatctg cgccccgatg
    17341 ccgtagaccc gcaggtccgc cggcggctgt gggtggtggc cgagcgccac cttgacgcgc
    17401 tcggagatgc tggtcggcga cggctcgcgc agcagcacga ccacacccct gcccgcctcg
    17461 ccgatcatcc gcatcgcctg gtggagctgg tcgccgcggc cgctctcgcc ttcgccgagc
    17521 aggtcctgca gcatgttgac ggcgtggacg cgcaccagga caggctccgg cgccgtgacg
    17581 tcgcccttga ccagggccac gtgctcgatg ccgtccagca cattgacata gacctggagg
    17641 cgaaaatcgc cgccgaagcg gctggtgaag gtggtggcga tgtcgcgctc gaccagccgg
    17701 tcgtgcttga gccgataggc gatgagatcg gcgatcttgc cgaccttgag gccgtggagc
    17761 tgggcgaagg cgacgaggtc gggcagccgc gccatggtgc cgtcgtcgtt catgatctcg
    17821 cagatcacgc cggacggatc gagcccggcg aggcgcgaga gatcgaccgc ggcctcggtg
    17881 tggccggtgc gcacgagcac ccccccatcc cgcgccatga gcgggaagat gtggccgggg
    17941 gcgacgaggt cgtcacgccg gctctcgggg tcgatggcca ccttgaccgt gtgcgcccgg
    18001 tcggccgccg agatgccggt ggtcaccccg tccctggcct cgatgctgat ggtgaaggcg
    18061 gtcgagaggc gcggctcgtt cccctgggcc atgagcggca ggcccagctt ctcgcagcgc
    18121 tgacggctca gcgccaggca gatcaggccg cggccgtgct tggccatgaa gttgatggcg
    18181 gcggcgtcgc agaactcggc cgggatgacc aggtcgccct cgttctcgcg attctcgtcg
    18241 tcgacgagga tgaacatctt cccgcgccgc gcgtcctcga gcacgtcctc gatcggcgaa
    18301 aggaagtcgg cgtcgctcaa gagcgatctc caccgggcac gaactcgagc tggcgggcga
    18361 cgtagcgggc gagcatgtcg gcctcgaggt tgacccgatc gccgacccgg cggtggcgca
    18421 aggtggtgac gtcccaggta tgcggaatga ccatcacggc aaagcccgtg tctgtcacct
    18481 cgttgacggt gagcgagacg ccgtcgaccg cgatggagcc cttgaccgcg accagcggcc
    18541 ggaggtcctc cggcatgtcg aaagcgaggc ggaagctctc gcccgaggga tcgatggcgg
    18601 cgatccggcc gagcccgtcg acatggccga agaccagatg gccgccgagc tcgtcgccga
    18661 ggcggagcga ggcttcgaga ttgatcgtat cgccctgctg ccaatcgccg agcgtggtgc
    18721 gcgccagggt ttcgggcgag gcgtagacgc tgtaggactc gaccgtcttc cccgcgacgg
    18781 tcaggcagac gccggaatgg gcgaccgagg cacctattgc aatacgcccg agatcgagtc
    18841 catcgggccg caccacgagc cggcggccgc cgtccttgtt ctcgacctcc aggatcatgc
    18901 cctggtgcgt gatgatgccg gtgaacattc ttggctttct ggcctaacct tcgggctcga
    18961 gcacgctgaa ccggtcgcgg ccgagcagcc gcgtgtcgac cacgcgccac cggccgacat
    19021 cggccatccg gtcgacgccg aggccctgga tggcagccag gccatcgccg ccgatcagcg
    19081 caggcgcgga gaagacgtag agccgatcga cgaggccggc ctcgagcagg ctcgtggcga
    19141 gcgacgctcc gccctcgacc agaagccgtg tgatgccttg gtcggcgagg cgggtcaaga
    19201 cggggccggg cgaggtgtcg ccatcggcgg ccgtgacatg gacgagcggc agccggtcgg
    19261 cgctggcgcg aagctggctc gtggcaggca gccggccgcg gcggtccatg acgacgcgga
    19321 ccggcgagcg gccctcgagg ccgggcaggc ggcaggtgag caggggatcg tcggcgagcg
    19381 ccgtgccgct gccgatcatg atggcgtcgt ggctggctcg caggcgatgg ccgaaggcgc
    19441 gggccgcctc gccggtgatc cactggctgt cgcccgtgcg ggtggcgatg cggccatcga
    19501 ggctcatcgc cagcttcaac ccgaccatcg gccggccggc ccggatgcgg cggtagaggc
    19561 ccagattctg cgccagcgcc tcggcctcgc gcacgccgac cacgacctcg agcccggctt
    19621 ccctgagccc ggcgatgccc ttgccgtcga cgcgcgggtc cgggtcgacg cagccgacga
    19681 cgacgcgggc gatgcccgcc tcgatcagcc ccttggtgca gggcggggtc ttgccccagt
    19741 tcgagcaggg ctcgagcgtg acataggcag tcgcacccct ggccgcgtcg ccggcacggg
    19801 ccagcgcctc ggcctcggca tgcggccggc cgcccggctg ggtccagccg cggccgacca
    19861 ccagcccatc cctgacgatg acgcagccga ccgatgggtt gggagcggtc aggcccagat
    19921 ggcgctcgcc cagggcaagg gccaggccca tgaagcgggc gtcgatcgga tcggtcatca
    19981 aaggacggtc acggctcggc ttcgtcggcc tcggggaggc gactgatgaa ctcctcgaaa
    20041 tcgcgcgcgt tggcgaaatc gcgatagacc gaggcgaagc ggacataggc gacctcgtcc
    20101 agcttggcca gggattccat ggccagctcg ccgatccgcg tggtcggcac ctcgtgctcg
    20161 ccgctggtct cgatgcggcg gacgatgccg ttgacgatgc gctcgatctg ctcctggtcg
    20221 acgccgcgct tgcgcacggc gatggcgatc gaacgcgcca gcttgtcgcg gtcgaagggg
    20281 acacgcttgt tgtccttctt cagcaccacg agatcgcgga gctgcacccg ctcgaacgtg
    20341 gtgaaccgcg ccccgcaggc cgtgcactgc cgccgccgcc tgatcgacgt cgcgtcctcg
    20401 accggccgcg aatccttcac ctgcgtgtcc tcggaaccgc aaaacgggca ccgcatcggc
    20461 tctgtcctgt gctcaccgcc ccagcatcgc cgcgttatag gcgcgctcgg ggccgagagc
    20521 cacccggggc tgccggggcc gaatgtctcg tgaagccgtc agtccggctc gcacacgaag
    20581 cccgactcct cacctcgatg gatgtagata tggccgacaa ggcgtccggc gctgccgagc
    20641 acaacccatc cccggccgca gaccggatcg ccctcgtcct ggccatccca ggtgaattcg
    20701 gcgcaggcgg cgccatcgcg cgcgccatag cggacgtcga gctggccctc gagcgcgccg
    20761 aaggcgattt cgccattgga cgcgcccttg aaggtgaggt gggcgggctg gacgagatcg
    20821 agatagtcgt cgtcgggcca ggtatccatc tcgacgatgc gccaccggcg cttgaatgcc
    20881 ttggcgaagg caggttgacg cttcatgcct tggcttcccc catcagcggc tcggacaatt
    20941 ccacgaaact ctcacctata cttcttcctc cgcctgctgg cgctgccaga gcgtggcgta
    21001 gagcccgtcc ttggccacca gctcggcgtg ggtgccgcgc tcgacgacgc ggccctcgtc
    21061 catgacgatg atctggtcgg cgtcgg
//
LOCUS       DAOTFI010000061        21056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3010_length_21056_cov_6.038332, whole genome shotgun sequence.
ACCESSION   DAOTFI010000061 DAOTFI010000000
VERSION     DAOTFI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3010_length_21056_cov_6.038332"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..3256
                     /locus_tag="P5558_08065"
     CDS             29..3256
                     /locus_tag="P5558_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRY24302.1"
                     /translation="MTAYAELQVTTNFCFLRGASHPEELVLRAHELGLAALAVTDRNS
                     FAGLVRAHVKAKELGLRFLPGVRLDLVDGPSLLAWPMDKPAYSRLSRLLTLGRRRAAK
                     GSCLLHLADVMAHDEGQALAVIDPDQATLEMLAGRWRDRLYLVATHRLDGGDRDRLAG
                     LASMARAAGVRLLATNDVHYHVPGRRPLQDVVTCIREHCTIAGAGGRLFANAERHLKP
                     AAEMARLFADWPEALASGLEIMRRCTFSMDELAYDYPVEESYDGRTPQQELERLTWEG
                     AVDCYGDPVPEKVRRILAHELALIAELAYAPYFLTVHEIVSFARSRAILCQGRGSAAN
                     SAVCFVLGITAVDPARIDLLFERFISAERNEPPDIDVDFEHERREEVIQHIYERYGRD
                     HAALTATVIRYRAKSAMREVGKVMGLSADVLDALSKSVWGYGRDGLRLDYVSEAGLDP
                     QDPLILKTMRLAEELIDTPRHLSQHVGGFVIARSPLHDLVPIENAAMANRTVIEWDKD
                     DIDALGMLKIDVLALGMLTCIRKAFDLLRQSYGIRVDLPAVPPEDPAVYEMLQDADSV
                     GVFQVESRAQMSMLPRLRPRTFYDLVIEVAIVRPGPIQGDMVHPYLRRREGREPVEFP
                     SGELEAVLGKTMGVPLFQEQAMRIAIVAAGFTPSEADGLRRAMATFKRRGQLERYRAK
                     LIEGMTAKGYAADFAERCFRQIEGFGYYGFPESHAASFALLVYVSAWLKCHYPDVFCA
                     AILNSQPMGFYAPAQLVRDAVDHGVEIRPVDVNRSFWDSTLEPLARRRGPLKTAVRLG
                     FRQIKGLSEAAALRLVTARLKPYSSPAELKRRAGLDHATLTRLAEADAFRSMAMDRRQ
                     ALWAVQALPDGELPLFAAAEAGAGFPGSNLAPLEHADEPWSELPEMGLGEHVIEDYTS
                     LRLSLKAHPLALLRDHLRPRRLATARQLWSIPPGRRVAIAGLVIVRQRPGSAKGVIFA
                     TLEDETGFANIIVWPKVFERYRAIVMRTRLLEASGRLQREGRVIHLVADRLTDLTPEL
                     HVLTRDSIDPAAFTDSLDRAMSPADEFKRAVPDPRDAMPKGRNFH"
     gene            complement(3264..4349)
                     /locus_tag="P5558_08070"
     CDS             complement(3264..4349)
                     /locus_tag="P5558_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY24303.1"
                     /translation="MKRRQLLPGLGKTLGLAALPPFLTACASVGSSDNPYYRGPVSDH
                     FDGRTFFNPGGAAPKGLGDFLKWKLGGDRAEWPAAYPSPFPAAVPAERLPSEALRLTM
                     VGHATLLIQTGGLNILTDPVWSERASPWRFIGPKRVNTPGVAFDHLPPIDIVLVTHNH
                     YDHLDVATLALLQQAHDPLVLTPLGNDTIIEAAVPAMRVEARDWDQSLSHGALTFHLE
                     PCHHWSARGLGDRRMALWAAFVIEGPAGRLYHVGDTGFDDGRNYRSAEARHGQFRAAI
                     LPVGAYEPRWFMAPQHQNPEEAVAGLLDCGAAHAAGIHWGTFQLTDEPIEAPLRALEA
                     ARAAQGLPPERFRPLRPGEVWDVPAVT"
     gene            4416..5054
                     /locus_tag="P5558_08075"
     CDS             4416..5054
                     /locus_tag="P5558_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY24304.1"
                     /translation="MVEKATALLNLYDRLILPWLIHRGMADERFRPLREELVAGTFGR
                     VLEIGIGSGLNLPFYGRAVEEVVGVDPSAALLERAGRQSPWLGFRLRLLRQSAEHLPF
                     DAATFDGAVVSWALCSISDPLVALAEVRRVLKPGGRLHFVEHGLAAAPGTARWQTRLT
                     PLWRRCAGGCRLDGRPDALLAAAGFRPGTLETGDLVPGPRLLTWHYRGTAVA"
     gene            5140..6015
                     /gene="aguB"
                     /locus_tag="P5558_08080"
     CDS             5140..6015
                     /gene="aguB"
                     /locus_tag="P5558_08080"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HRY24305.1"
                     /translation="MRQVTVAATQMPCGPDRASNLAGAESLVRRAAAAGAQIVLLQEL
                     FESVYFCKDQSPAHFSLAYSFEGHPLLARMSRLAAELGVVLPVSFFERANAAYFNSLA
                     MIDADGKVMGVYRKSHIPDGPGYQEKFYFSPGDTGFRIWQTKFGCIGVGICWDQWFPE
                     AARAMAVMGAELLFYPTAIGSEPQDPRLDSREHWRRAMQGHAASNCMPVIASNRIGRE
                     VGESCAINFYGSSFITDATGGITAELGQEPGLVTASFDLDELARTRAAWGLFRDRRPD
                     LYLPLLTLDGRDAGI"
     gene            complement(6019..6867)
                     /locus_tag="P5558_08085"
     CDS             complement(6019..6867)
                     /locus_tag="P5558_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24306.1"
                     /translation="MSDHPAIAPGRVAVVTGAASGIGLAAAGRFAERGMKLVLADVDG
                     AALTAAAEGLRQRTGRPADILAVPTDVGDRAAVEALKDRAYGAFGEVAVLVNNAGREG
                     GGQLLGDPARWQTILQTNLWGVVNGIQVFAPAMIAQGTTGAIISTGSKQGITCPPGDT
                     AYNVSKAGVKVATEALAHELRNTEGCKVTAHLLIPGFTFTGFTKVRHKEKPAGAWLPG
                     QVVDELLAGMARNDFYILCPDNDVSRALDEKRILWAAGDIVHNRPPLSRWHPDYKAAF
                     ERFLEE"
     gene            complement(7090..7896)
                     /locus_tag="P5558_08090"
     CDS             complement(7090..7896)
                     /locus_tag="P5558_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24307.1"
                     /translation="MACIRARFAAPGLAALLCAAGPVAPRAADALVSEGWQVQLTPYF
                     WALAMEGNTRVRGIKGEPDVSFEDIWDNLDYAVMLEGEVRKGRFGMFANVIYADLGDT
                     QNVDGFDVKGEATVSWAGFGGFYRLGPWTVLPEAGTLAPKVVVDPYAGVRYTYADVTL
                     DVRNGGPQGSDDQDWVDPIVGVRTIWQIGSKWSVTALGDIGGFGVGSDFTWQAAGMIG
                     YSFDLFGDDNARLLAGYRALYQDYSSGSGDNEFEWDMTLHGPVLGLAIAF"
     gene            complement(7905..8933)
                     /locus_tag="P5558_08095"
     CDS             complement(7905..8933)
                     /locus_tag="P5558_08095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY24308.1"
                     /translation="MIARRAKHIAAAAVLTLLAACSTTESLIQPADPLPSWNDGATKE
                     AITTFVEAVTTEGGPDYVAPADRIATFDNDGTLWPSHPMYTQLVFALDRIKVLAPQHP
                     EWKTTQPFKDVLDGNLEGLAGSGEKGIAELVMATHAGMSTADFEAIVSDWFTTAKHPH
                     FDRLYTDLAYQPMLELLDYLRANEFTTYIVSGGGVEFMRPMTEEVYGIPAQQVIGSSI
                     VTKFEMQDGRPVLMREAKVDFIDDKDGKPVGINKFIGKRPIAAFGNSDGDREMLEWTA
                     GGDGARLMMLVFHDDAEREFAYGPANGQPDTKFGTFSQALMDEAKSSGWNVISMKDDW
                     GTIFAASE"
     gene            complement(8956..9924)
                     /locus_tag="P5558_08100"
     CDS             complement(8956..9924)
                     /locus_tag="P5558_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24309.1"
                     /translation="MSRSRRMIARLASGLLVVALGACAGTTRDDVQPPPPASSQPASP
                     PAADKDAAPGGAAGAGSPHGNLAAVGQKLADPTSDVWALFTEFDWNFVQGEATSEYRH
                     AQTMLFQPVLPVPLTDDLKMITRPVVPVASVPVPDGTGGVDRKTGLADIQLPLLLVPK
                     QGVSLGGNSLTYGAGPTFVFPTATSDDLGSDRWEAGPALVGVLKNQHFTGGALAQYWW
                     SYAGGSPSTSHGSLLYFFFYSLPDAWQIGFNPTITYDHEASSGNKWSVPIGLTVAKTT
                     RIGGTPVKFQLGGEYFVVNQDDYGPRFLLKLNIIPVIPALIKEPLL"
     gene            complement(10036..11688)
                     /locus_tag="P5558_08105"
     CDS             complement(10036..11688)
                     /locus_tag="P5558_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRY24310.1"
                     /translation="MNGSRLLGAAIPIALAAMLAGPMPAAAQDAERPNILLIVSDDTG
                     WGDLGPYLGGEGRGMPTPNFDRLADEGMVFTNFYGQPSCTPGRAAIQTGRIPNRSGMT
                     TVAFQGQGGGLPAAEWTLGSVLKEAGYKTYFTGKWHLGEADYALPNAQGYDVMKYAFL
                     YHLNAYTYPDPKWHLAMSPELRQMFEKITTGALSGNAGEKAKEVWKVNGEYVDTPDKG
                     VVGIPYLDAYVEEAALEFLDDAAKDPDTPFFINVNFMKNHQPNIPHPDFEQKSISKSK
                     YADSVVELDARVGNVLKKLDELGLADNTVVFYTVDNGAWQDVYPDAGYTPFRGTKGTV
                     REGGNRVPSMVRWPNHVAGGSVSHDILGGLDLMATFASLAGSELPKNDRENKPIIFDS
                     YDMTPVITGAGPSDRKDWFYFTEDELSPGAVRVGPFKYVFNLRGDDGAETGGLSVDTN
                     LGWKGAESYVATVPQVFDLLDDPQERYDIFMTTFTESTWAVVPANESVQKLMKTYVEY
                     PPRKLQSETYAGPITLTQYQRFKYVRDALAKDGFNLSLPSGN"
     gene            12203..14047
                     /locus_tag="P5558_08110"
     CDS             12203..14047
                     /locus_tag="P5558_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24311.1"
                     /translation="MAAYTSHATIDEAIDAAQPPRDVVREQLRRILAEPTFQASGPRR
                     AFLRFVVEETLAGRADRLKGFTIAQAVFDRDDDFDPQTDPVVRVEARRLRRDLDGYYA
                     GPGRCDQLIISIPKGGYLPRFDTREIEADGLAGTTGSSTAMSGMSRTAFLGELTRSGQ
                     LHVRFGWATAGLLALLLLVAIVMGIHQPRQSGEEAAGSPRLPSVLVRPFLAADRSVST
                     GILADGITNELVSNLMRFPDFRLYLAPLGHDERDAADLDRRVAEDVSYLVSGEMVLDG
                     DQFRLSAHLVDVASGRVIWSETYARDLTTMNLVELQADLAGEIASTLGQPYGVVRNDI
                     LARVGTYVPTELESYYCVLRAYQHRRSLDERERGGVRACIEAAVRRDPAYAEAWAMLA
                     WHHLDEARFPAAKSPDAAERAYTLAFSAASKALALDPENVQALKSLSAIHHYRGNYEE
                     GWRLARLALERNPHDPDTLAQLGWRLAIRGRFEEGEALLRRAIDRTISPPGWYYHLIT
                     IEHMMRGEYAEMLATAQLGAASGGSMAHSFVAVAEANLGRPEAARAALLRMAEASPAW
                     AADPATNFRIHGATDEIVEALMSGLRRAGWHGAKAAAMPEGVVAPVVH"
     gene            14117..15166
                     /locus_tag="P5558_08115"
     CDS             14117..15166
                     /locus_tag="P5558_08115"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HRY24312.1"
                     /translation="MISRLRSAAGKLLRLLAKPVRRTDSQGPIVIDTFRGYASKDALF
                     MMGRVMHQPEGDGPGDFSDSASLWNVIRRMRRRGVSGARLIATVDSQRQGLITDRDGY
                     FSLRMRFRQPLLPHRATARVHLQLIKPAIAEAVGEVLVAPPSARFMVISDFDDTVVAT
                     GVANKIRMISRMLMRSAESRVAFAGVAELYRQLGDGAGGHEQNPLLYVSRGPWVIYET
                     LEAFFKLHRFPERPVLFLREWGLRFRPPFVRSDHDHKIEIINELMAVSPDLPVVLIGD
                     SGQHDPEMYDDVIRSWPGRVRLVLIRDVTSGARDEAVAELAQHPDVPFVLAETSAEMA
                     AAAAAAGLIETGRRG"
     gene            15375..18830
                     /locus_tag="P5558_08120"
     CDS             15375..18830
                     /locus_tag="P5558_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY24313.1"
                     /translation="MKFAKVLARVTWLLVTENTVSYQRLRRDFGLDDGALEDLRRQLV
                     LIHKVATDQGGESLAWAHAASSGTASTGLAPIRPSAPVAAAVATSQPAATVSDAERRP
                     LTVMFCDLADSTALSARLDPEDLQDVIRAYQETCTGLIQAYGGFVAKYMGDGILVYFG
                     YPKSLEKNAERAVRTGLAIVEAMGGLNAGPGRAKGIEIAVRIGIATGLVMVGEVVGEG
                     LAQERTVIGEAPNVAARLQSLAGRNGIVIGALTKEIAGDAFLYEDLGARELKGITGLV
                     VAWGVAGLRDAGDERADEAETESGAPPPTLVGRDEEVGLLRRAWQGTKDERRGQVVLI
                     SGEAGIGKSVLIDGLRAEVLAEGLPRVTFRCSPYHTNSALYPVIEHFKRLAGWQPEDA
                     PAARVAKLEAMIEHYDQPLGETLPLLASLLSLPLPEERYPPLTLTPQQQKQQTQDAII
                     AMTLEAAEQQPLLELWEDLHWADPSTLELLELLIEQAPTASLLMVLTARPEFVPPWPA
                     RSHITPITLNRLERQHSEALVNRLAGTRPLPAEVVEHIVAKTDGVPLYVEELTKTILA
                     SDILRDTGGRYELTGPLASLAIPDTLQESLMARLDRLPQVRELAQLGSVLGREFAYEM
                     ITGLATVGETALQAGLGQLVASELLYQRGRPPRARYIFKHALVQDAAYQSLLRRTRQQ
                     HHQQVAELLESRFPEVVRVQPELLARHYSEAGVTERAVTYWLEAGRRAAGRSAYPEAI
                     AHLHQGLAMLADLAETPERARQELAFQSTLGPVVMATQGYGSSAAAVIYHRARELCRL
                     VGDTEVIYPVLFGVYLLDLVSARYRESIEVANELLELARQAGNSGARAAGHLALGVSL
                     VQAGQPSLACEHFEQSIALLDQEERRPIAFRYGLEVGATANAYWAWSLWLLGYPDRAR
                     ELGGQTLALLGEVRHPYTESRGLYWNSIVHAFRREWAIVDERAAEAMRSATEHGFALV
                     LAAGRIMQGAALAAGGQGAAGLQEMQDGLDAYRASGAVFQRSFHLTLLAEVLGAAGSP
                     EAGLEALQAAEELVGTTGERFWEPEIFRLKGELLLAAPGGTPAVAEACFHQALDLARR
                     QEVKSLELRAATSLARLWAGQGERGKAQDLLAPVHGWFTEGFDTADLVEARALLDELA
                     AAAPT"
     gene            complement(18840..19328)
                     /locus_tag="P5558_08125"
     CDS             complement(18840..19328)
                     /locus_tag="P5558_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24314.1"
                     /translation="MSRTGPRTRGLGRLVALGFALLLAACAESRPVPEPPAGRTPAGR
                     APAGTVELREVQVARPGNAGGGRGTLAYRGRTYPFAIAGLGVDGGDAVDAEGDVYNLH
                     DLADFSGTYTAGQYGAVVGDAGTGDIWLENERKVILHLKAEHEGLRLAVDGAAVDIRL
                     KN"
     gene            complement(19343..19696)
                     /locus_tag="P5558_08130"
     CDS             complement(19343..19696)
                     /locus_tag="P5558_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRY24315.1"
                     /translation="MHGTMSTAAIAALMLLAVAATRPALAHNDWGLPLIGGLVGGYAL
                     KSFMGDGGGGHEHERSAPAAAPPAPAAAPAAAPAADSASSIEQKLNTLDQLAAKGYIT
                     KDEYNARRQALLNQL"
     gene            19854..20300
                     /locus_tag="P5558_08135"
     CDS             19854..20300
                     /locus_tag="P5558_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24316.1"
                     /translation="MTTRGRTIAVLGMHIGAVAIAGGVLADEVPEGYLPGSWAVGSAE
                     ACAAGTGERLTFGADGAFSGTLHGSPTAAGFWQRDEDLLMLHMVASPAFFDDPATDAD
                     DALAAHAGQYSYYFARGLMFDIEPDAFRVVATMGDQMQGANLFRCP"
     gene            20291..>21056
                     /locus_tag="P5558_08140"
     CDS             20291..>21056
                     /locus_tag="P5558_08140"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24317.1"
                     /translation="MSLSTLPDGRGTDRPGAGRWLARLVVLAVLVAATLPWTAAAGEV
                     EQAIGARVAALGATGHGEAGGVAIEAVPFVQDFYGARQDRPAWLDTVAGEELLAAIAA
                     SGRHGLRPVDFHGDRLPALLDAARSGDPAAIAAFELVATDAAALLLHHLYRGKVDATR
                     LDPDWNFHRPVVTGESAGTISVLVDQLGFAAIVGRVALQHPQYLALQDALERYRTIEA
                     AGGWPRVPPGETLRPGMADPRVPALRARLVVTGDLAP"
BASE COUNT         3105 a         7133 c         7437 g         3381 t
ORIGIN      
        1 gaccgcctgg ctgccgggcg ctgagccctt gaccgcctac gccgagctcc aggtgaccac
       61 gaacttctgc ttcctgcggg gcgcctcgca cccggaggag ctggtgctgc gcgcccacga
      121 gctggggctc gccgcccttg ccgtcaccga tcgcaacagc ttcgccgggc tggtgcgggc
      181 gcatgtcaag gcgaaggagc tgggcctgcg cttcctgccg ggcgtgcggc tcgacctcgt
      241 ggacggcccg tcgctgctcg cctggcccat ggacaagccg gcctacagcc gcctctcgcg
      301 gctcctgacg ctcggccggc ggcgggcggc caagggcagc tgcctgctcc acctcgccga
      361 cgtcatggcg catgacgagg gccaggccct cgccgtcatc gaccccgacc aagccacgct
      421 cgaaatgctg gccgggcgct ggcgcgaccg gctctacctc gtcgccacgc accgcctcga
      481 cggcggtgac cgggatcgcc tcgccggtct cgcttccatg gcccgggcgg cgggcgtgcg
      541 cctgctcgcc accaacgacg tgcactatca tgtgccaggg cggcgcccgc tccaggacgt
      601 cgtcacctgc atccgcgagc actgcaccat cgccggggcg ggtggccggc tcttcgccaa
      661 tgccgagcgg cacctcaagc ccgcggccga gatggcccgg ctcttcgccg actggcccga
      721 ggcgctggcc agcggcctgg agatcatgcg gcgctgcacc ttctccatgg acgagctggc
      781 ctacgactac ccggtcgagg agagctatga cggccgcacc ccgcagcagg agctcgagcg
      841 cctgacctgg gagggtgccg tcgactgcta tggcgacccg gtgcccgaga aggtccggcg
      901 catcctggcc cacgagctgg cgctgatcgc cgagctcgcc tatgcgccct atttcctcac
      961 tgtccacgag atcgtcagct tcgcccgctc gcgcgccatc ctctgccagg gcaggggctc
     1021 ggcggcgaac tcggccgtct gcttcgtcct cggcatcacc gccgtcgacc cggcccggat
     1081 cgacctcctc ttcgagcgct tcatctcggc cgagcgcaac gagccgcccg acatcgacgt
     1141 cgatttcgag cacgagcgcc gcgaggaggt gatccagcac atctacgagc gctacggccg
     1201 cgaccacgcg gcgctgaccg ccaccgtcat ccgctaccgg gcgaagagtg cgatgcgcga
     1261 ggtcggcaag gtgatggggc tctcggccga cgttctggac gccctttcga aatcggtctg
     1321 ggggtatggc cgggacgggc tccggctcga ttacgtcagc gaggccgggc tggatccgca
     1381 ggaccccctg attctcaaga ccatgcggct cgccgaggag ctgatcgaca cgccccggca
     1441 tctctcgcag catgtcggcg gcttcgtcat cgcgagaagc ccgctccacg atctggtgcc
     1501 gatcgagaac gccgccatgg cgaaccgcac cgtgatcgaa tgggacaagg acgacatcga
     1561 cgctctcggc atgctcaaga tcgacgtgct cgccctcggc atgctgacct gcatcaggaa
     1621 ggccttcgat ctcctgcgcc agtcctatgg catcagggtc gacctcccgg ccgtgccgcc
     1681 ggaggacccg gccgtctacg agatgctgca ggatgccgat tcggtgggcg tcttccaggt
     1741 cgaatcgcga gcgcagatgt cgatgctgcc gaggctcagg ccgcgcacct tctacgatct
     1801 cgtcatcgag gtggcgatcg tccggccggg gcccatccag ggcgacatgg tccaccccta
     1861 tctccgccgg cgcgagggca gggagccggt cgagttccct tcgggggagc tcgaggcggt
     1921 cctgggcaag accatggggg tgccgctctt ccaggagcag gcgatgcgga tcgccatcgt
     1981 cgccgccggc ttcacgccat cagaggccga cgggctcagg cgggccatgg cgaccttcaa
     2041 gcggcgcggc cagctcgagc gctaccgggc caagctgatc gagggcatga ccgcgaaggg
     2101 ctacgccgcc gacttcgccg agcgctgctt caggcagatc gagggcttcg gctattacgg
     2161 cttccccgag agccacgccg ccagcttcgc gctcctcgtc tacgtctcgg cctggctcaa
     2221 gtgccactac ccggacgtct tctgtgctgc catcctcaac agccagccca tgggcttcta
     2281 cgccccggcg cagctggtgc gtgatgccgt cgaccatggc gtcgagatcc ggccggtcga
     2341 cgtcaaccgg agcttctggg acagcacgct cgagccgctg gcccgccggc gggggccctt
     2401 gaagaccgcc gtccggctcg ggtttcgcca gatcaagggg ctaagcgagg cggcggccct
     2461 gcgtctcgtc accgcccggc tcaagcccta tagcagcccg gccgagctca agcgccgcgc
     2521 cgggctcgac cacgccacgc tgacccgcct cgccgaggcc gacgccttcc gctccatggc
     2581 catggaccgc cgccaggcgc tctgggcggt gcaggcgctg ccggacggcg agctgccgct
     2641 cttcgccgca gcggaggccg gcgccggctt ccccggctcc aacctggcgc cgctggagca
     2701 tgccgacgag ccttggtccg agctgccgga gatgggcctc ggcgagcatg tcatcgagga
     2761 ctacacgagc ctccgcctgt cgcttaaggc gcaccccctg gcgctcctgc gcgaccatct
     2821 ccggccgcgc cggctggcga ccgcccgcca gctctggtcg atcccgcccg gccgccgcgt
     2881 cgccatcgcc ggcctcgtca tcgtccgcca gcgcccgggc agtgcgaagg gcgtcatctt
     2941 cgccacgctc gaggacgaga cgggcttcgc caacatcatc gtctggccca aggtcttcga
     3001 gcgctaccgg gcgatcgtca tgcgcacccg cctgctcgag gcgagcggcc ggctgcagcg
     3061 cgagggccgg gtcatccacc tcgtcgccga ccggctcacc gacctcacgc ccgagctcca
     3121 cgtcctgacc cgcgacagca tcgatccggc ggccttcacc gactcactcg atcgcgccat
     3181 gagccccgcc gacgagttca agcgggcggt gccggatccc cgggacgcca tgcccaaggg
     3241 gcggaacttc cattgaccgg aggtcaggta accgcaggca cgtcccagac ctcccccggg
     3301 cgcagcgggc ggaagcgctc gggtggcagg ccctgtgccg cgcgggcggc ttcgagggcg
     3361 cgcagcgggg cctcgatggg ctcgtcggtg agctggaacg tgccccagtg gatgccggcg
     3421 gcgtgggcgg cgccgcaatc gaggaggccg gcaacggcct cctccgggtt ctggtgctgc
     3481 ggcgccatga accagcgcgg ctcgtaggcg ccgaccggca ggatcgccgc ccggaactgc
     3541 ccgtggcgcg cctctgccga gcggtagttg cggccatcgt cgaagcccgt gtcgcccaca
     3601 tggtagagcc ggccggccgg gccctcgatg acgaaggcgg cccagagcgc catgcgccgg
     3661 tcgccgaggc cgcgggccga ccagtggtgg cagggctcga ggtgaaaagt cagcgcgcca
     3721 tggctgaggc tctggtccca gtcccgggcc tcgacccgca tcgccggcac ggccgcctcg
     3781 atgatcgtgt cgttgccgag cggagtcagc accaagggat cgtgcgcctg ctgcagcagg
     3841 gccagcgtcg ccacgtcgag atgatcgtag tgattgtggg tgaccagcac gatgtcgatg
     3901 ggcggcaggt ggtcgaaggc gacgccgggc gtattgaccc gcttcgggcc gatgaagcgc
     3961 caggggctgg cgcgctcgga ccagaccggg tcggtcagga tgttgagccc gccggtctgg
     4021 atcaagagcg tcgcgtggcc gaccatggtc agccgcagcg cctcggacgg caggcgttct
     4081 gccgggacgg cggcggggaa tgggctcgga tacgctgccg gccactcggc ccggtcgccg
     4141 ccgagcttcc atttgaggaa gtcgccgagc cccttggggg cggcgccacc cggattgaag
     4201 aaggtccggc cgtcgaaatg gtcgctgacc ggcccccggt agtagggatt gtcgctcgag
     4261 ccgaccgagg cgcaggcggt caggaagggc ggcagggcgg cgagcccgag ggtcttcccg
     4321 agtcctggaa ggagctggcg tcttttcatc gggccgcaga taaggcattg cgactgccgg
     4381 tcaagccggc gccggctcga ccggccgatg cgcgaatggt ggaaaaggcg acggccctgc
     4441 tcaatctcta cgaccgactt attctgccct ggctgatcca tcgtggcatg gccgacgagc
     4501 gttttcgacc gttgcgcgag gagctggtcg ccggcacgtt cggccgggtt ctggagatcg
     4561 gcattggctc ggggctgaac ctgccgttct atgggcgcgc ggtcgaggaa gtcgtcggtg
     4621 tcgatccctc ggcggccctg ctcgagcggg ccggccggca gagtccgtgg ctcggctttc
     4681 gtctccgcct gctgcggcag agcgccgagc acctgccctt cgacgccgcc accttcgatg
     4741 gcgccgtggt gagctgggcg ctctgctcga tctccgatcc gctggtggcg ctggccgagg
     4801 tccgtcgggt gctgaaaccc ggcgggcggc tgcacttcgt cgagcacggg ctggcggcgg
     4861 cgccgggcac tgcccgctgg cagacgcggc tgacgccgct ctggcggcgc tgtgcgggcg
     4921 gctgccggct cgacgggcgg ccggacgccc tcctggccgc agccggtttc cggcccggca
     4981 cgctggagac cggcgatctc gtcccaggcc cgcggctcct tacctggcat tatcggggca
     5041 cggccgtggc ctgagccggg agtccgactg ccgcggtttt cctctttgcc aagcccgagc
     5101 cgaggcggca tagtccgcgg cttccgacgg ggagatagca tgcgccaggt gaccgtcgcc
     5161 gccacccaga tgccctgtgg gccggaccgc gcgagcaatc tcgcgggtgc ggagagcctg
     5221 gtgcgccggg ccgccgccgc gggtgcgcag atcgtgctgc tgcaggagct cttcgagtcg
     5281 gtctatttct gcaaggacca gtcgccggcg catttctcgc tggcctattc gttcgagggc
     5341 catccgctgc tcgcccgcat gagccggctc gccgccgagc tcggcgtggt cctcccggtc
     5401 agcttcttcg agcgggccaa tgccgcctat ttcaactctc tggcgatgat cgacgccgac
     5461 ggcaaggtga tgggggtcta ccgcaagagc cacatcccgg acgggccggg ctaccaggag
     5521 aaattctact tctcgcctgg cgacaccgga tttcgtatct ggcagaccaa attcggctgc
     5581 atcggcgtcg gcatctgctg ggaccagtgg tttcccgagg ccgcgcgggc catggccgtg
     5641 atgggggccg agctgctctt ctacccgacc gcgatcggca gcgagccgca ggacccgcgg
     5701 ctcgacagtc gcgagcactg gcgccgggcg atgcagggcc atgcggcgag caactgcatg
     5761 ccggtgatcg cctcgaaccg gatcggccgc gaggtcggcg agagctgtgc catcaacttc
     5821 tatggcagct cgttcatcac ggatgccacg ggcgggatca ccgccgagct cggccaggag
     5881 ccgggcctcg tcaccgccag cttcgatctc gacgagctgg cccgcacccg cgccgcctgg
     5941 ggcctcttcc gcgaccgtcg cccggacctc tatctgccct tgctcaccct cgacggccgc
     6001 gacgccggca tctgaggctc attcctcgag gaagcgctcg aacgccgcct tgtagtcggg
     6061 gtgccagcgc gagagcggcg ggcggttatg gacgatgtcg ccggccgccc agaggatcct
     6121 cttctcgtcg agcgcgcggc tgacgtcgtt gtcggggcag aggatgtaga agtcgttcct
     6181 cgccatgccg gccaggagct cgtcgacgac ctgccccggc agccaggcgc cggccggctt
     6241 ctccttgtgc cggaccttcg tgaagccggt gaaggtgaag cccgggatga gcaggtgagc
     6301 ggtaaccttg cagccctcgg tgttgcgcag ctcgtgcgcc agcgcctcgg tcgcgacctt
     6361 cacaccggcc ttgctcacat tgtaggcggt gtcgccgggc gggcaggtga tgccctgctt
     6421 ggagccggtc gagatgatgg cgccggtggt gccctgcgcg atcatggccg gggcgaagac
     6481 ctggatgccg ttgaccacgc cccagagatt ggtctggagg atcgtctgcc agcgcgccgg
     6541 atcgccgaga agctggccgc cgccctcgcg gccggcattg ttcacgagca ccgccacctc
     6601 gccgaaagcc ccgtacgccc ggtccttcag cgcctcgacg gcggcacggt cgccgacatc
     6661 ggtcggcacg gcgagaatgt cggccggccg gccggtccgt tgccgcaggc cctcggcagc
     6721 ggcggtcaag gccgcgccat cgacatccgc cagcacgagc ttcatgcccc gttcggcgaa
     6781 ccgcccggcg gctgccagcc cgatcccgct cgccgccccg gtgacgacgg cgacccggcc
     6841 gggagcgatg gctggatggt cggacatggc ggtattcctc gaggttgctg tggtgacgag
     6901 ggtatgaagc gggaggggac gaagggcaac cggagcgggg cgaaagggag catggtgcgc
     6961 gatcagggat cggctccgaa gaaggactgc caatctgtta tcgccgtctc gtcaacagcg
     7021 cgagatcgtt cgcccatcgg tttttctcgc catccgaccg tgtcggggcg gtgaaagagc
     7081 tcctgccgct cagaacgcga ttgcgaggcc gaggaccggt ccgtgcaggg tcatgtccca
     7141 ctcgaactcg ttgtcgccgc tgcccgacga atagtcctgg tagagcgccc ggtagccggc
     7201 caggagtctg gcgttgtcgt cgccgaagag gtcgaagctg tagccgatca tgccggcggc
     7261 ctgccaggtg aagtcggagc cgacgccgaa cccgccgatg tcgccgagtg cggtgacgct
     7321 ccacttcgag ccgatctgcc agatcgtccg cacaccgacg atcggatcca cccagtcctg
     7381 gtcgtcgctg ccctgcggcc cgccgtttcg gacatccaat gtgacgtccg cgtaggtgta
     7441 gcggacaccg gcatagggat cgacgaccac cttcggggca agggtcccgg cttcgggcag
     7501 cacggtccag ggacccagac ggtagaagcc gccgaacccg gcccaggaga ccgtggcttc
     7561 acccttgacg tcgaagccgt cgacgttctg cgtatcgccg agatcggcgt agatcacgtt
     7621 ggcgaacatg ccgaagcgac ccttgcgcac ctcgccctcg agcatcacgg cgtagtcgag
     7681 attgtcccag atgtcctcga aggacacatc gggctcgccc ttgatgccgc ggaccctggt
     7741 gttgccctcc atcgccagcg cccagaaata gggtgtcagc tggacctgcc acccctcgga
     7801 gacgagggca tcggctgcgc gcggcgccac cggcccggct gcgcagagga gtgctgcgag
     7861 gccgggcgcg gcgaagcggg ctctgatgca ggccaaggcg ggactcactc ggatgcggcg
     7921 aagatggtgc cccagtcgtc cttcatgctg atcacgttcc agccgctgct cttcgcctcg
     7981 tccatcagcg cctgcgagaa cgtgccgaac ttggtgtcgg gctggccgtt cgccgggcca
     8041 taggcgaact cgcgctcggc gtcgtcgtgg aagaccagca tcatcaggcg cgcaccgtcg
     8101 ccgccggcgg tccattccag catctcgcgg tcgccgtccg agttgccgaa ggcggcgatc
     8161 gggcgcttgc cgatgaactt gttgatgccg acgggcttgc cgtccttgtc gtcgatgaag
     8221 tcgaccttgg cctcgcgcat caggacgggc ctgccgtcct gcatctcgaa cttcgtgacg
     8281 atgctggagc cgatcacctg ctgggcgggg atgccgtaga cctcctcggt catcggccgc
     8341 atgaactcca cgccgccgcc cgagacgatg taggtcgtga actcgttggc gcgcaggtag
     8401 tcgagcagct cgagcatcgg ctggtaggcg aggtccgtgt agagacggtc gaaatgcggg
     8461 tgcttcgccg tggtgaacca gtcgctgacg atcgcctcga agtcggcggt gctcatgccc
     8521 gcgtgggtcg ccatgaccag ctcggcgatg cccttctcgc cggatccggc gagcccttcc
     8581 aggttgccgt cgaggacgtc cttgaagggc tgggtcgtct tccactccgg atgctgcggt
     8641 gccagtactt tgatgcggtc gagcgcgaag acgagctgcg tgtacatcgg atgggacggc
     8701 cagagcgtgc cgtcattgtc gaaggtggcg atccggtcgg cgggggcgac atagtcgggg
     8761 ccgccctcgg tggtcaccgc ctcgacgaag gtcgtgatcg cctccttggt ggcgccgtcg
     8821 ttccaggacg gcagcgggtc cgccggctga atgagcgact cggtggtcga gcacgcagcc
     8881 agcagcgtca ggacggcggc agcggcaata tgtttcgctc ttcgggctat catcattgtt
     8941 tccttccttc ggggatcaca ggagcggctc cttgatcagg gccgggatca ccgggatgat
     9001 gttgagcttc agcaggaatc gggggccgta gtcgtcctgg ttgacgacga aatactcgcc
     9061 gccgagctgg aacttgaccg gcgtgccgcc gatccgggtg gtcttggcga cggtgaggcc
     9121 gatcggcacg ctccacttgt tgcccgagct ggcttcatga tcgtaggtga tggtcgggtt
     9181 gaagccaatc tgccaggcgt cgggcaggct gtagaagaaa aagtagagga gggagccgtg
     9241 gctggtggac gggctgcctc cggcgtacga ccaccaatac tgcgccagcg cgcctcccgt
     9301 gaaatgctgg ttcttgagga cgccgacgag cgccggcccc gcctcccacc tgtcgctgcc
     9361 cagatcgtcg ctcgttgcgg tgggaaagac gaaggtcggc ccggcaccgt aggtcaggct
     9421 gttgccgccg agcgagacgc cctgcttcgg cacgaggagc aggggcagct ggatgtcggc
     9481 gaggccggtc ttgcgatcga cgccgccggt cccatccggc accggcacgc tcgccacagg
     9541 caccaccggc cgggtgatca tcttcagatc gtcagtaagc gggaccggca ggaccggctg
     9601 gaacagcatg gtttgcgcat ggcgatactc ggaggtcgcc tccccctgga cgaagttcca
     9661 gtcgaattcg gtgaacagcg cccagacatc cgaagtcgga tcggcgagct tctggccgac
     9721 cgcggcgagg ttgccgtgcg gcgaaccggc gcccgcggcc ccgcccggcg ccgcatcctt
     9781 gtcggcggcg ggtggcgagg ccggctggct gcttgcgggc gggggtggtt gaacgtcatc
     9841 gcgggtggtg ccggcgcaag cgcctagggc aaccaccagc agacccgacg ctagccgggc
     9901 gatcatgcgt ctcgatcggg acatctacgc tcccaagtgg ccgcggtcct gtcgggttac
     9961 cgaggccgtg ggcacccgcg gtgccggaaa cccattttcg gggttcccga ggccgcaggt
    10021 gccgctaaag caacgtcagt tgccggaagg cagcgacagg ttgaagccgt ccttggccag
    10081 cgcatcgcgg acatacttga accgctggta ctgggtgagc gtgatcggtc ccgcgtaggt
    10141 ctcgctctgc agcttgcgcg gcgggtactc gacataggtc ttcatgagct tctgcaccga
    10201 ctcgttggcc gggaccacgg cccaggtgct ctcggtgaag gtcgtcatga agatgtcgta
    10261 gcgctcctgc gggtcgtcca ggagatcgaa cacctgcggc accgtggcga cgtagctctc
    10321 ggcacccttc cagcccagat tggtgtcgac cgagaggccg cccgtctccg ccccgtcgtc
    10381 gccccggaga ttgaagacgt acttgaaggg accgacgcgc accgcgcccg ggctcagctc
    10441 gtcctcggtg aagtagaacc agtccttgcg gtcggacggg ccggcgccgg tgatgaccgg
    10501 ggtcatgtcg tagctgtcga agatgatcgg cttgttctcg cggtcgttct tcggcagctc
    10561 gctgccggcg agcgaggcga aggtggccat cagatcgagg ccgcccagga tgtcgtggct
    10621 gacgctgccg ccggcgacat ggttcggcca gcggaccatc gacggcacgc ggttgccgcc
    10681 ctcgcgtacg gtgcccttgg tgccgcggaa gggcgtgtag ccggcatccg ggtagacgtc
    10741 ctgccaggcg ccgttgtcga ccgtgtagaa gacgacggtg ttgtcggcga ggcccagctc
    10801 gtcgagcttc ttcagcacgt tgccgacccg cgcgtcgagc tcgaccacgg agtccgcgta
    10861 cttggacttc gagatggatt tctgctcgaa atccggatgc gggatgttcg gctggtggtt
    10921 cttcatgaag ttgacgttga tgaagaaggg cgtgtcaggg tccttcgcgg cgtcatcgag
    10981 gaactcgagc gcggcctcct cgacataggc gtcgaggtag ggaatgccga ccacaccctt
    11041 gtccggtgtg tcgacatact cgccattgac cttccacacc tccttggcct tctcgccggc
    11101 attgcccgag agggcgccgg tggtgatctt ctcgaacatc tggcgcagct ccgggctcat
    11161 cgccaggtgc catttcggat ccgggtaggt gtaggcgttg aggtgataga ggaaggcgta
    11221 cttcatcacg tcgtagccct gggcgttggg cagcgcgtag tccgcctcgc ccagatgcca
    11281 cttgccggtg aagtaggtct tgtagccggc ctccttgagg accgagccga gcgtccactc
    11341 cgccgccggc aggccgccgc cctggccctg gaaggccacg gtggtcatgc cgctgcggtt
    11401 ggggatgcgg ccggtctgga tggcggcgcg gccgggcgtg cagctcggct ggccgtagaa
    11461 attggtgaag accatgccct cgtcggcgag gcgatcgaag ttcggcgtcg gcatgccgcg
    11521 cccctcaccg ccgagatagg gcccgagatc gccccagccc gtatcgtcgg agacgatcag
    11581 gaggatgttg ggcctttcgg catcctgcgc cgcggcgggc atgggacccg ccagcatggc
    11641 tgcgagcgcg atcggaatcg ccgcccccag cagtcgacta ccgttcatgc gatcttcctc
    11701 cgttcgattg agaatgcgtc tgtcaacagc aacgaccgga ccgatcgttg cgtggctccc
    11761 ggctttcgcc cgggttcagg aagtcgaggg cctcggcctc gagctccgcc agcaatcttt
    11821 ggcactccgc gatcctcccg ggcaagggag ggtcgaccgt ccgccagtgc tcgagcgccc
    11881 gccggactgc cttgaggtcg tcgcagacgg cgcggaactc cgtctcgcaa agatggaatc
    11941 gccgcatggc cagctcgtgc tccggatggc gttgcgacaa gcgatggacg ctcgccactg
    12001 tacgacctcc ccgagccaag tgacggggcc agtggacaga gcgtcccatc cgagggcgca
    12061 ggcatgaagg tgcgaacagg ttcgcacctg acgcgtggat cggtgcaaac ctcgatcgaa
    12121 caagggtttc gatcgtcggg gcgcactgct agtgtggaat aagacttgga atcgccgacc
    12181 ggcctttttt aggtgagctt ctttggccgc atacacgagc cacgccacaa tcgacgaggc
    12241 gatcgacgcc gcacagccgc cgcgcgacgt ggtgcgggag cagctgcggc gcatcctggc
    12301 agagccgacc tttcaggcga gcggaccgcg gcgcgcgttc ctgcgcttcg tcgtagaaga
    12361 gacgcttgcc ggtcgcgccg accgactcaa ggggttcacg attgcgcaag ccgtgttcga
    12421 ccgcgacgac gatttcgacc cgcagaccga cccagtcgtc cgtgtcgagg cgcgtcgcct
    12481 gcgccgcgat ctggacggct actacgccgg acccggccgt tgcgatcagc tcattatatc
    12541 cattccgaaa ggaggatatc ttccccgctt cgatacacgg gagatcgagg ccgacggcct
    12601 agcaggcacc acagggtcct ccactgcgat gtccgggatg tcgcggacag cttttttggg
    12661 cgagttgacg cgctccggtc agctccatgt gcgttttgga tgggcgaccg ccggcctcct
    12721 cgcactgctg ctcttggtcg cgatcgtcat gggcattcac cagccgcggc agtccggcga
    12781 agaagcagcc ggtagtccga gactcccctc ggtgctggtc aggccgttcc tcgcggcgga
    12841 ccggtcggtt agcaccggca tcctcgctga tgggatcacg aacgagctcg tcagcaacct
    12901 gatgcgcttc cctgatttcc ggctctactt ggctccgctt ggtcacgacg agcgcgacgc
    12961 agcggatctc gatcgccgcg tggccgagga cgtatcgtat ctggtgagcg gcgagatggt
    13021 cctcgacggt gaccagttcc gtctttccgc gcatctcgtc gatgttgcga gcggacgagt
    13081 gatctggagc gagacttatg cgcgggacct gaccaccatg aacctcgtcg aattgcaggc
    13141 ggatctggcg ggcgagatcg cctcgacact cgggcaacct tacggcgtgg tccgcaacga
    13201 tatccttgcc cgcgtgggca cgtacgtacc cactgaactg gagagctact attgcgtgct
    13261 cagagcctac cagcaccgac gaagccttga cgagcgcgag cgtggcggtg tgcgcgcctg
    13321 catcgaggcg gccgtccggc gtgatccggc ctatgccgag gcctgggcga tgctcgcctg
    13381 gcaccatctc gacgaagccc gctttccggc cgcgaagtca ccggacgcgg ccgagcgcgc
    13441 ctacacgctt gccttctccg ctgcgtcgaa agctctcgcc ctcgatccgg agaatgtgca
    13501 ggccctgaaa tcgctctccg cgatccatca ctatcgcggc aactacgaag agggctggcg
    13561 gctcgctcgc ctggcgctcg aacggaaccc gcacgatccc gacactctcg cgcaactcgg
    13621 ctggcgcctc gccatccgag gccggttcga ggagggcgag gccttactac gccgggccat
    13681 cgaccgcaca atcagcccgc cgggctggta ctaccatctg atcactatcg agcacatgat
    13741 gcgtggcgag tatgccgaga tgctggcgac cgcacagctg ggcgctgcca gcggaggatc
    13801 catggcgcac tcttttgtgg cggtagcgga ggcaaatctc ggcaggccgg aagcggcgcg
    13861 cgcggcgctc ctgcgaatgg ccgaggcctc gccggcctgg gcagccgacc ccgccaccaa
    13921 cttccggata catggggcga cggacgagat cgtcgaggcg ctgatgtcgg ggttgcggcg
    13981 cgccggctgg cacggtgcga aggcggcggc catgccggag ggagtcgtgg cgccggtcgt
    14041 tcattgagca ggcttgcccc cggcggcggc caatgaccac cttcccttcg ggctcgactg
    14101 gtgccattgc ggcggcatga tctcgagact tcgctccgcc gccggcaagc tgctgcggct
    14161 cctggccaag cccgtgcgcc gcacggacag ccagggccct atcgtcatcg acacctttcg
    14221 cggctacgcg tccaaggacg cgctcttcat gatgggccgc gtcatgcacc agccggaggg
    14281 cgacgggccg ggcgacttct cggattccgc cagcctgtgg aacgtgatcc ggcgaatgcg
    14341 gcggcggggg gtgagcggtg cccggctgat cgcgacggtc gacagccagc ggcagggcct
    14401 gatcaccgac cgcgacggct atttcagcct gcgcatgcgg ttcaggcagc cgctcctgcc
    14461 gcatcgcgcc accgccaggg tgcatctcca gctcatcaag ccggccatcg ccgaagcggt
    14521 cggcgaggtg ctggtggcac cgccctcggc ccgcttcatg gtcatcagcg acttcgacga
    14581 caccgtcgtg gcgaccggtg tcgccaacaa gatccggatg atctcgcgca tgctgatgag
    14641 gagtgccgag agccgggtcg ccttcgccgg cgtcgccgag ctctaccggc agctcggcga
    14701 cggcgccggc ggccacgagc agaacccgct gctctacgtc tcgcgcggcc catgggtcat
    14761 ctacgagacg ctcgaggcgt tcttcaagct gcaccgcttt cccgagcgcc ctgtgctgtt
    14821 cctgcgcgaa tggggcctgc gctttcgacc gcccttcgtg cgcagcgacc acgaccacaa
    14881 gatcgagatc atcaacgagc tcatggccgt ctcgcccgac ctgccggtcg tgctgatcgg
    14941 cgacagcggg cagcacgacc cggagatgta cgacgacgtc atccggagct ggcccggccg
    15001 ggtgcgcctc gtcctgatcc gcgacgtcac gagcggcgcc cgggacgagg cggtggccga
    15061 gctggcgcag catccggacg tgcccttcgt gctggccgag acctcggccg agatggccgc
    15121 cgccgccgcc gccgcggggc tgatcgagac gggccgccgc ggctgatcgg ccggcccggc
    15181 accgatggtt caccgcgacg ctgcggccat tgtcagcgat gtcgggcagt gggcagcgcc
    15241 cgagccgggt gacgcgccct gcatgtggcc cacaaatccc aatagtgaaa tgctggtccg
    15301 cggcgcataa gcggatgagc aaacaaaagc tgtccgatac gccgggcaag gtgcccggca
    15361 tggcgtgagg catcatgaag ttcgccaagg ttctggcacg agtgacgtgg cttctcgtta
    15421 ccgagaacac ggtctcttat cagcgcctca ggcgggattt cgggctggac gatggtgcgc
    15481 tggaggatct tcgccggcag ctcgtcctga tccacaaggt ggcgacggac cagggcgggg
    15541 aatcgctggc ctgggcccat gcggcgagct cgggcacggc atcaacgggc ctggcgccga
    15601 tccggccatc ggcgccggtt gcggccgccg tggcgacgtc gcagccggcg gccacggtca
    15661 gtgacgccga gcggcggccg ttgacggtga tgttctgcga tctggcggat tcgacggcgc
    15721 tgtcggcgag gctggacccc gaggacctgc aggacgtgat ccgcgcctat caggagacct
    15781 gcaccgggct gatccaggcg tatgggggct tcgtcgccaa atacatgggt gacgggatcc
    15841 tggtctattt cggctatccg aaatcgctgg agaagaacgc cgagcgggcg gtgcggaccg
    15901 gcctcgcgat cgtcgaggcg atgggcgggc tgaacgccgg gccggggcgg gccaagggga
    15961 tcgagatcgc ggtccggatc ggcatcgcca cgggcctcgt gatggtcggc gaggtggtcg
    16021 gcgaggggct ggcgcaggag cggacggtga tcggcgaggc gccgaacgtc gccgcacggc
    16081 tgcagagctt ggccggccgc aacggcatcg tgatcggtgc cctgaccaag gagatcgcgg
    16141 gcgacgcgtt tctttacgag gacctgggtg cgcgggagct gaagggcatc accgggctgg
    16201 tcgtcgcctg gggggttgcc ggcctgcgcg atgccggtga cgagcgggcg gacgaggccg
    16261 agaccgagag cggggcgcca ccgccgacgc tggtggggcg ggacgaggag gtcgggctgc
    16321 tgcgccgggc ctggcagggc accaaggacg agcgccgcgg ccaggtggtg ctgatcagcg
    16381 gcgaggccgg gatcggcaag agcgtgctga tcgacgggct gcgcgccgag gtgctcgccg
    16441 agggactgcc ccgggtcacc tttcgctgct cgccctatca caccaacagc gcgctctatc
    16501 cggtgatcga gcatttcaag cggctggccg gctggcagcc ggaggacgcc ccggcggcgc
    16561 gggtcgccaa gctcgaggcc atgatcgaac actatgacca gccgctcggc gagacgttgc
    16621 cactgcttgc ctcgctgctg tcgctgccgc tgccggagga gcgctacccg ccgctgacgc
    16681 tcacgccgca gcaacagaag cagcagaccc aggatgcgat catcgcgatg accctggagg
    16741 ccgccgagca gcagccgctg ctcgagctct gggaggacct gcactgggcc gacccctcga
    16801 cgctggagct gctcgaactc ctgatcgagc aggcgccgac cgcgtcgctc ctgatggtgc
    16861 tgaccgcccg gcccgagttc gtgccgccct ggccggcgcg ctcgcacatc accccgatca
    16921 ccctcaaccg gctggagcgc cagcacagcg aggccctggt gaaccggctc gccggcacga
    16981 ggccgctgcc ggccgaggtg gtcgagcaca tcgtcgccaa gaccgacggt gtgccgctct
    17041 atgtcgagga gctgaccaag acgatcctcg cctcggacat cctgcgcgac accggcgggc
    17101 gctacgagct caccgggccg ctggcgtccc tggcgattcc ggacacgctg caggaatcgc
    17161 tgatggcgcg cctggaccgc ctgccgcagg tgcgcgagct ggcgcagctc ggctcggtgc
    17221 tggggcgcga gttcgcctac gagatgatta ccggcctcgc cacggtcggc gagacggcgc
    17281 tgcaggccgg gttggggcaa ctggtggcgt cggagctgct ctaccagcgt ggccgtccgc
    17341 cacgcgccag gtacatcttc aagcatgcgc tcgtccagga cgccgcctac cagtcgctcc
    17401 tgcggcgcac ccgccagcag caccaccagc aggttgccga gctgctggag agcaggttcc
    17461 ccgaggtggt cagggtgcag cccgagctct tggcccgcca ctacagcgag gcgggcgtca
    17521 ccgagcgggc cgtgacgtac tggctcgagg cgggccgccg cgcggccggg cgatcggcgt
    17581 atcccgaggc gatcgcccac ctgcaccagg ggctcgccat gctggcggac ctggcggaga
    17641 cgccggagag ggcgaggcag gagctcgcct tccagagcac gctcggcccg gtggtgatgg
    17701 cgacccaggg ctatggctca tcggcggcgg cggtgatcta tcatcgcgcg cgcgagctgt
    17761 gccggctggt gggcgacacc gaggtcatct atccggtgct gttcggcgtc tacctgctcg
    17821 acctggtgag cgcgcgctac cgggagtcga tcgaagtcgc caacgagctc ctggagctgg
    17881 cgcggcaggc cgggaattcg ggcgcccgcg cggcgggcca cctggcgttg ggcgtcagcc
    17941 tggtgcaggc ggggcaaccg tcgctggcgt gcgagcattt cgagcaatcg atcgccctgc
    18001 tcgatcagga agagcgtcgg cccatcgcct ttcgctatgg cctggaagtc ggggccacgg
    18061 ccaatgccta ctgggcctgg tccctctggt tgctcggcta tccggatcgg gcccgcgaac
    18121 tcggtgggca gacattggcg ctgctcgggg aggtcaggca tccctatacc gagtcgcgcg
    18181 gcctgtactg gaactccatc gttcacgcct tccggcggga atgggcaatc gtcgacgagc
    18241 gcgcggctga ggcgatgcgg tcggcgaccg agcacggctt cgcgctggtg ctcgcggccg
    18301 gtcggatcat gcagggtgcc gccttggccg ccggcggcca aggggccgcc ggactgcaag
    18361 agatgcagga cggtctcgac gcctatcggg cctcgggcgc cgtctttcag aggtcgttcc
    18421 acctgacgct gctggcggag gtgctcggtg ccgcggggtc gccagaggcc gggctcgagg
    18481 ccttgcaggc ggccgaagag ctggtcggga cgaccggcga gcgcttttgg gagccggaga
    18541 tatttcgctt gaaaggggag ctgctgctgg ccgcaccagg cgggaccccg gccgtcgccg
    18601 aggcatgctt ccatcaagcg ctcgacctcg ctcgccgtca ggaggtgaaa tcgctggagc
    18661 tccgtgccgc aaccagcctc gcccgtctct gggccgggca gggcgagcga gggaaggccc
    18721 aggacctgct ggcgccggtc cacggctggt tcaccgaagg cttcgacacg gccgatctcg
    18781 tcgaggccag ggcactgctc gacgagctgg ctgccgccgc accgacctga gccgccggct
    18841 cagttcttca gccggatgtc gacggcggcg ccgtcgacgg cgagcctcag gccctcgtgc
    18901 tcggccttca aatggaggat gaccttcctc tcgttctcga gccaaatgtc gccggtgccg
    18961 gcgtcgccga caaccgcacc gtactggccg gcggtgtagg tgccggagaa gtcggcgagg
    19021 tcgtggagat tgtagacgtc gccctcggca tcgacggcat cgccaccgtc gacgccgagc
    19081 ccggcgatgg cgaaggggta ggtccggccg cggtaggcga gggtgccgcg gccgccgccg
    19141 gcattgccgg gacgggcgac ctggacctcg cgcagctcga ccgtgccggc gggggcgcgg
    19201 cccgcgggag tcctcccggc cggcggctcc ggcactggcc tgctctcggc gcaggcggcc
    19261 agcagcaggg cgaagcccag ggccacgagg cggccgaggc cgcgcgtgcg ggggccggtg
    19321 cgcgacatgc ggcggtgccc gatcagagct ggttcaggag cgcctggcga cgggcgttgt
    19381 actcgtcctt ggtgatgtag cccttggcgg cgagctgatc gagcgtgttc agcttctgct
    19441 cgatcgagga tgccgagtcg gcggccgggg ccgcggcggg cgctgccgcc ggggccggcg
    19501 gggccgcagc gggcgcgctg cgctcgtgct cgtgcccgcc gccgccgtcg cccatgaagc
    19561 tcttcagcgc atagccgccg accaggccgc cgatgagcgg cagcccccag tcgttgtgcg
    19621 ccagggccgg ccgtgtcgca gcgaccgcca gcagcatcag ggcggcgatg gccgccgtcg
    19681 acatcgtgcc gtgcaccttc gttcctcccg caccggttct cgaccgccag tcgtcgatgg
    19741 ccgccgcggc ggccgccggc tggcgccgat tccatatagc gttggtcggc gaaatgaacc
    19801 atgattccgt gcatcggagg aagcgagcag gccacagtgt gggtagcaag ggaatgacga
    19861 cacggggtcg cacgatcgcg gttctcggca tgcatatcgg tgccgtggcc atcgccggcg
    19921 gggtcctcgc ggacgaggtg ccggaaggct acctgcccgg aagctgggcc gtcgggtcgg
    19981 ccgaggcctg cgccgccggg acgggcgaac ggctcacctt cggcgccgac ggcgcgttct
    20041 cgggcacgct gcacggcagc ccgacggcgg ccggcttctg gcagcgcgac gaggacctgc
    20101 tcatgctgca catggtggcc tcgcccgcct tcttcgacga cccggcgacc gacgcggacg
    20161 acgcgctcgc cgcccatgcc gggcagtatt cctactattt cgccaggggc ctgatgttcg
    20221 acatcgagcc cgatgccttc cgcgtggtcg ccaccatggg cgaccagatg cagggcgcca
    20281 acctcttccg ttgtccctga gtacgttgcc ggacggccgg ggcacggacc ggccaggggc
    20341 ggggaggtgg ctcgcccgcc tcgtcgtgct ggcggtgctg gtggcggcga ccctgccctg
    20401 gacggcagcg gcgggcgagg tcgagcaggc gatcggggcc cgggtcgcgg ccctcggggc
    20461 gacgggccac ggtgaggccg gcggcgtcgc gatcgaggcc gtgcccttcg tccaggactt
    20521 ctacggcgcc cggcaggacc ggccggcctg gctcgacacg gtggccggcg aggagctcct
    20581 ggccgcgatc gccgcgagcg ggcggcacgg gctccggccc gtggatttcc acggcgaccg
    20641 cctgccggcg ctgctcgacg ccgcccggag cggcgatccc gccgccatcg ccgccttcga
    20701 gctggtcgct accgatgctg ccgccctgct gctgcaccac ctctatcgcg gcaaggtgga
    20761 cgccacccgg ctcgatccgg actggaactt ccaccggccg gtggtgaccg gggagtccgc
    20821 ggggacgatc agcgtgctgg tcgaccagct gggcttcgcc gcgatcgtcg ggcgggtggc
    20881 gctgcagcat ccgcaatacc tcgccctgca ggacgcgctc gagcgctacc gcaccatcga
    20941 ggctgccggc ggctggcccc gggtgccgcc gggcgagacg ctgcggccgg gcatggccga
    21001 cccgcgcgtg ccggcgctgc gtgccaggct cgtggtcacg ggcgacctcg cgcccg
//
LOCUS       DAOTFI010000062        20695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3095_length_20695_cov_6.796463, whole genome shotgun sequence.
ACCESSION   DAOTFI010000062 DAOTFI010000000
VERSION     DAOTFI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 20695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20695
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3095_length_20695_cov_6.796463"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="P5558_08145"
     CDS             complement(<1..909)
                     /locus_tag="P5558_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24318.1"
                     /translation="MPVEPTYPGAHIEEIPSGVRTITGVATSVAAFVDRFARGPLGKP
                     VRLVGVADLERELGGLSPTSEASYAIQQFFINGGSECWAVRVADTTSSTAPATAASAT
                     LRAQPGAGATVFTVSAGRRVDEASVADPGIWGNDLFLEIDHDTADPTTTFNLTVSEIV
                     TRNGRRVTKRSESHRNLVLKTSGPGNAVETLNAASSMIQLTPAAGFPGHLPAATGTMG
                     VAIAAGARIPATPVALTVDAGSGARIVSVAHSLGATSEDHAAFRPLLEAAIRAAASAA
                     TTDDERSLIEGAVVRLIGEGTAASPHR"
     gene            complement(1015..1455)
                     /locus_tag="P5558_08150"
     CDS             complement(1015..1455)
                     /locus_tag="P5558_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY24319.1"
                     /translation="MLVEKLLPRTRKRMQCIELGAALLQAARLLDDGQDMLLVRDEEG
                     RLAGILTKTDVVRQIGRCAGASCTAPVAQAMTRDVVSVRPDGWLQEAWEAMKERGFKN
                     LPITDDAGRPLGVLNAREALQALLDETRHEEELLRDYVMNVGFH"
     gene            complement(1502..1789)
                     /locus_tag="P5558_08155"
     CDS             complement(1502..1789)
                     /locus_tag="P5558_08155"
                     /inference="COORDINATES: protein motif:HMM:NF040453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6522 family protein"
                     /protein_id="HRY24320.1"
                     /translation="MTEIEYDDETIQIDAEVLAGALRIDTDELLRRMREGTVTSRLER
                     GEGEDAGRIRLTFFSGSRRVRMVADESGKILTCGAVDYGERPLPARLRRAG"
     gene            complement(1812..2249)
                     /locus_tag="P5558_08160"
     CDS             complement(1812..2249)
                     /locus_tag="P5558_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY24321.1"
                     /translation="MRLATSTDFGLRVLMRLAAAPGAALSTGRIADELQVSAHHLAKV
                     VRALGRGSFVGSQRGRHGGLQLARPAEAITVGEVVRHLEQRFAMVECFRADGGHCVLA
                     PSCRLKPQLALAREAFMQALDQTTIADCAWPGAGLPIGAASAH"
     gene            2338..3006
                     /gene="ric"
                     /locus_tag="P5558_08165"
     CDS             2338..3006
                     /gene="ric"
                     /locus_tag="P5558_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRY24322.1"
                     /translation="MTPTTQAPHDRPVGEIAARLPGASGVFRQYGIDFCCHGDVPLQQ
                     AATSRGLDPRVVLEALNALDPAAAPLAPEETGALIDHIQTRYHDVHRRQIPELIELSS
                     KVELVHLTHPDVPTGLADALERILGELEVHMKKEELILFPAMRRQAAARLATPIGEMR
                     HDHDSHGRFLEEMARLTDDCVPPQGACRSWQALYAGAAQLRADLMEHIHLENNVLFPR
                     FEAA"
     gene            3118..3357
                     /locus_tag="P5558_08170"
     CDS             3118..3357
                     /locus_tag="P5558_08170"
                     /inference="COORDINATES: protein motif:HMM:NF022969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HRY24323.1"
                     /translation="MSEQWLPSLVTPTPQEGFDLAVKLSRMAVKLTQPSAEVREALRG
                     GYAHDASDLIAVSHVVATHFQTIAAANGYWRTDRG"
     gene            3487..4515
                     /gene="rhaS"
                     /locus_tag="P5558_08175"
     CDS             3487..4515
                     /gene="rhaS"
                     /locus_tag="P5558_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY24324.1"
                     /translation="MTRILHPTRRQLTGAMALGAAGLTLPLGFGRRARAADRRIAMIV
                     KNLGNSYFDACASGGAEAAAELGGTELIYTASTKPTAEDQIAVIDSLIAQRVDGIIVS
                     ANDANALVPVGKKAMQRGIKVMSFDSAIAPEGRLVHLSASSTDLIGAKQVQMIAKTLN
                     NEGEVAILSAASTMTNQNAWIEAMKVEWAKPEYANMPLVATVYGDDQDDKSYREMQAL
                     VKAHPNLKGVISPTTIGIRAGAKAIMDEGLVGQVYITGLGLPSEMEAAVLAGACDSFA
                     IWNPVDYGYAATMIMGAILDGAGTAEGDTLPMGRIGETVVGAEGEAVMGEPFVFDKTN
                     IEEYAQIF"
     gene            4591..6090
                     /locus_tag="P5558_08180"
     CDS             4591..6090
                     /locus_tag="P5558_08180"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY24325.1"
                     /translation="MAPVLSLSHISKGFPGVQALDDVSLTLERGSVTALIGENGAGKS
                     TIVKILTGIYTPDAGTVNVEGRDRRFATPKDAWAAGIAAIHQETVMFDELSVQENIFM
                     GHMPGGRLIDRTAIRDRTKELLARIDADFAPETPLKRLSVAQKHMVEIARALSHEAAV
                     IIMDEPTASLSRHEIGELFSLVDQLRDEGRAILFISHKFDEIFRVSDRWVCLRDGRKV
                     GEGLTAEVTEPELVRLMVGRPIDQVFPKRDVVIGEVVLEVEGLSNATEFADISFELRR
                     GEILGLYGLVGAGRSEAMQCVFGLSQPTRGRIVMEGRPLAPRAPGDAIDAGISYVPED
                     RQEQGVVLPLGIRANITLASLARHARGWFLSSSSELDATRALGDRLAVKAASWEQPVM
                     ELSGGNQQKVVIAKWLATQPRVIILDEPTKGIDIGSKAAVHAFIGELVADGLAVVLIS
                     SELPEVMGLADRILVMHEGRIVARFARDAWSAEAIVAAATGAVAADRAA"
     gene            6087..7082
                     /locus_tag="P5558_08185"
     CDS             6087..7082
                     /locus_tag="P5558_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24326.1"
                     /translation="MSWLAAHREVVLALVIALMVAAIGAYAPAFVEGTSLLGVLDDTA
                     LLFMLALGQMVVILTRGIDLSMAANLALTGMLVALVGQHGPDLPALAYIVLAILIGLG
                     FGLGNGVLIGGLGIPPIVVTLGTLSIFRGMIVVIGGGDQVNASEMGRTFQAFPHKALL
                     GLSSVFWIALAVAILMQLFLSRTRTGRGIYAVGGNPTAARYCGLVLERYQVLVYAIAG
                     GIAGLSGYLWVARYGVAYSEIASGFELTIIASCVIGGVSIGGGVGTVPGTLLGALFIG
                     VIVNALPTMQVSPFWQMAISGAAILAAVIINARAEAHPDKLILPEARRAAAGARG"
     gene            7088..8095
                     /locus_tag="P5558_08190"
     CDS             7088..8095
                     /locus_tag="P5558_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24327.1"
                     /translation="MPDSPRYTVADRPPFRLADLLLRWESILVVLLALVFAVNAWATP
                     WFLDIYNLADATYNFSEKAIIALAMALLILTREIDLSVAAIVALAALGVGAAGALGQP
                     TPVLVLTGLGIGLLCGLCNGVLVTRFGVHSIVITIGTMSLYRGIAQVSLGDQALTAFP
                     QPFLDLQQTYFLADPPTPLSFVLFLALAVVFGIVLHLTATGRRLFAIGANPVAARFSG
                     IRVDRIRFLLFTVSGLMAGLAGVLLTARIGVVRPNIALGWELDVVTMVILGGVAISGG
                     IGSIPGVVLAVLVLGFATFGLTLLNVPGIVINVLLGCLLIGSIALPILIRRAMRRRRS
                     T"
     gene            complement(8135..8614)
                     /locus_tag="P5558_08195"
     CDS             complement(8135..8614)
                     /locus_tag="P5558_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRY24328.1"
                     /translation="MSKNTKALENLQQALSMELTAVHQYLMHAHVLDDWGLDRLAAKM
                     REEMQEELGHAGAFIDRILFLKGDPRIEAGKTPRRATSLEEMFKADLAEEQGAIAFYT
                     EAADAAAEESDIGTRTLFEQTLLDEEGHSAWLELQLDLLERMGQGAYIAKHMSAPGD"
     gene            8773..10344
                     /locus_tag="P5558_08200"
     CDS             8773..10344
                     /locus_tag="P5558_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRY24329.1"
                     /translation="MSNPELGRGGPLLVGVDAGTTNTKAIIIDLAGCVVAEASEPTRI
                     EHPRPEWAEYAAETLWEVAASAIRRAVNQLDDPDRIQGVAVASMAETGVPLDGSGAAT
                     GPAIAWFDKRARAELALIEERVGPDRLFEISGLAPEPIFGLCKLLWHKHHRPDSFART
                     VQWLNAADYLAWRLSGEMATDYSLASRTFALDLGTQRWSTEVLEAVGVDASLMAPLVR
                     SGQRVGKVQAEAARATGLPRHCVVAAGGHDHMVGSVAADAMRPGVMLLSTGTTEAILM
                     GRDTPGKDPALGRVGYAQGLMWVDEPVYYVIGGVFTAGGAIEWFRRTLGAGAGAEGAD
                     YATLVAEAAAAPPGSLGALFLPQLRLGTPPHIDGHARGAFLGLSTDVTHGCLFRAVLE
                     GIAADSRLCVEGMLRLLPADPPREVRIIGGLTRNPLYMSIRSAVAGRPLSVVDLPDGV
                     AAGAALLAGLGAGLYPTLEASLEAMRPPEREVTPDAAMAGLYDRLVREVYSKAYPTVR
                     ALHEAAQKAISAPPR"
     gene            complement(10322..11314)
                     /locus_tag="P5558_08205"
     CDS             complement(10322..11314)
                     /locus_tag="P5558_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24330.1"
                     /translation="MPRTRLEKKLARIRKDPSGAKDFLICDAKDGDMGGGIPMPGVAR
                     DAKGRSTGRFKSRFDHLAQVEALVEQDILDMVLLSVGNLDHLKRRGVFEKSRVGTAIR
                     ANDTTDIWGARHSRYKQQPSLPHRTASVAHAKALCDLGLYSITFMNDAAIDAAAGAEY
                     RAFRHECEEHGFKHFLEVFNPNVGMDKATAVEIGEFVNDNIVRVFAAVPDAQRPEFLK
                     IVYNGPKALEELVNYDPTLIVGILGGGAGTTRDTFELLKASQKGGARVVLFGRKINLA
                     EHPLALVALMRRLVEGEVGPEEAVRAYHGELARAKIKPVRALEQDLEVTEAVLK"
     gene            complement(11808..12728)
                     /gene="ppk2"
                     /locus_tag="P5558_08210"
     CDS             complement(11808..12728)
                     /gene="ppk2"
                     /locus_tag="P5558_08210"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRY24331.1"
                     /translation="MTDERQSGREKSWVEAELMDSFDEELEMEIDDERIARELRSITD
                     HEHKSSVERRTYFLELFRLQAELIRLQDWVAHTGYKLVVLFEGRDSAGKGGVIKRITQ
                     RLNPRVCRVVALPAPTERERTQWYFQRYVPHLPAGGEIVLFDRSWYNRAGVERVMGFA
                     SEAQVQEFFTDVPEFERMLVRSGIKLVKYWFSITDQEQQLRFLMRIHDPMKQWKLSPM
                     DLESRVRWEAYTKAKEEMLERTNIPEAPWSIVAGNDKKQARLNCIHHLLRQVPYGDVP
                     HDPINLPDRVFNPDYERKVLPDALYVPEVY"
     gene            complement(13244..14437)
                     /locus_tag="P5558_08215"
     CDS             complement(13244..14437)
                     /locus_tag="P5558_08215"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRY24332.1"
                     /translation="MTERTGLVLVINAGSSSLKFRVFEPATAASGAAVLASGQIDGIG
                     VRPRFVAEDGSGEELARVTDMAVLDHDGAIRLLIEWLGERFGGRGIAAVGHRVVHGGT
                     EHAAPVLVDDRVMAELRKLVPLAPLHQPHNLGAIEAVAAAYPGVPQVACFDTAFHRGH
                     PWVADTFALPRHFYDEGLRRYGFHGLSYEYIARELARTAPALAAGRVVVAHLGNGASC
                     CAMLAGRSIDSSMGFTALDGLPMGTRTGQIDPGVLLHLIDERGFDTARLQTLLYHESG
                     LKGLSGISSDMRDLEASDRPEAEEAIAYFAYRARCQIAAQAAALEGLDGLVFTAGIGE
                     HGHGVRARICRGLAWLGIELDEAANAEGATIVSTAKSAVEVRVVPTDEERMIALHALG
                     VLERS"
     gene            complement(14459..15259)
                     /gene="fabI"
                     /locus_tag="P5558_08220"
     CDS             complement(14459..15259)
                     /gene="fabI"
                     /locus_tag="P5558_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRY24333.1"
                     /translation="MSDSEILTFDPQARTLEGKRGVVLGVANRQSIAYGCAAAFRFLG
                     AELALTYLNEKARPHVQPIAEELEAPILMPCNVEREGELDAVFEAAGKRWGSIDFALH
                     SIAFAPKEDLQGGLLDSSGPGFGRAMDISCHSFVRMARCAAPLMTNGGTLFAMSYYGA
                     EKVVPHYNVMGPVKAALECSVRYLAHELGPRRIRVHAISPGPIRTRAASGINEFEELL
                     NAAETRAPSNRLVSIRDVGAATAALATPAGQLITGNTIYVDGGYHVMG"
     gene            complement(15712..17832)
                     /locus_tag="P5558_08225"
     CDS             complement(15712..17832)
                     /locus_tag="P5558_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY24334.1"
                     /translation="MSQPSTEPASIAPDIRATPPRLWRFLVISAVLVAGTLGLGLGLV
                     ELLVRSRATVLQGMVEARLAALASSRAESLDTWLAGHVGRADRLAASPTIRLYLAEQV
                     HAADDPRLAAALLEQRPYMRQVLDSFVDQQGLEAAQLLGADGSTVVADRNGRQAATSD
                     EPRAIPVAGAGYRQLRQQGESLLLDLVQPIAPPLDLAEEATAAPVGALAVTIDATARL
                     AALLEHRPTDLEGEESRLVVELQSGSTMLARSDAGERWTAIPFGGSLPTPGWSMDLVA
                     TASVPGTDWIVVQSIDKASAFATLAEFQRTARLIGVAISLAVALACCALWWLREARHR
                     RLLAEQYRALAERLAAANRILTTITDHAAELVGLKDAQGHYRFVNPTFARHLAMPAAA
                     VIGRTDEELFPAAVAEALRANAEDAAAGGAAPSLELETGDPARPLLLEVTQAPVPGAS
                     VGIEGTVLIGRDVTERALERRLREELAGQTCHAFMRAIGTVDPYLVGHAEAVREVAQA
                     IGTVLDLGADERATLGRAAELSQLGKVFLPRSLLAKPERHTEADMALMRTHIDHTLRI
                     IGSIDFALPVADVLAEMHERLDGTGYPHGRFAAEISLPGRILAAADVFCARIRPRSYR
                     EAESAETVVRHLLEQHDRYDPVVCRTLAGLLPRLAHLATPATGAHEPSVEPAATPLGP
                     VEARPAPRRTPGAKATGELAMPVG"
     gene            complement(17885..18577)
                     /locus_tag="P5558_08230"
     CDS             complement(17885..18577)
                     /locus_tag="P5558_08230"
                     /inference="COORDINATES: protein motif:HMM:NF017815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HRY24335.1"
                     /translation="MPRWLLAIVMTLLPIAAAEATVPVALFGTAEFRAESLAALPKWR
                     QVLERSALEQPTYDACRLDPTACPNRGVMAWQALLQGLRGAPLPEQVSKVNRFVNQWR
                     YRSDAANHGKSDYWASPLEFLARSGDCEDYAIAKYVSLRQLGVPAARLRLVVVQDELR
                     QLAHAVLAVYLDDTAVILDNLTNAVLPHERVAHYAPYYSVNETARWAHVESSRLMLSA
                     VDRSSTTAPASR"
     gene            complement(18683..19774)
                     /gene="flhB"
                     /locus_tag="P5558_08235"
     CDS             complement(18683..19774)
                     /gene="flhB"
                     /locus_tag="P5558_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRY24336.1"
                     /translation="MAEEQDDSQKTEDPSQKRLDDAREKGQTASSREVSNLLLIGSLT
                     LVLAIALPGAMRDLALALSHYLRHAGDPTLGAAALGGSLRRALAAMGLALLVPLLVTI
                     VASVAAPALQGALVWTAQSMMPKAERISPIAGFKRLFSLKSLVEFIKGLVKVGLMTVI
                     GVVVVWPDRQRLVASGQLEIAAALVVLWEMAIRVSGAIAACLVLVAGLDYAYQRFEFM
                     KQMRMSRRDLQDEHKQSDGDPVVKQRLRSLRMEKARRRMMAEVPTSTVVITNPTHFAV
                     ALRYEPGETPAPKLVAKGVDSLALRIRELAQEHDVPIIESPPLARALHRSVELGALIP
                     QQHYQAVAEIIGYVMRLRQAGGGVRGSVR"
     gene            complement(19779..20543)
                     /locus_tag="P5558_08240"
     CDS             complement(19779..20543)
                     /locus_tag="P5558_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRY24337.1"
                     /translation="MNPLDLLLAREITVIGLVFCRVGAALMVLPAFGEPYVVSRVRLG
                     IALAIALLVGLPMSGRLVLPTDVAGLTLLVIAEVLTGLLIGAIIRLVMFAIHFGGSLV
                     AMQSGLATAAFFDPQEATQGTVTGNFLAILALVVIVVSDAHLLVLQALTTSFSVLETT
                     GSLDLAGLVEVLVRLGGEAIETGLRMAAPVVLFSLLLYLLLGVLNRLMPSFQVLFVAM
                     PLQIVLAFGLFMLSLGTVVGLATTLLRTTMTWLEPV"
BASE COUNT         3065 a         7409 c         7025 g         3196 t
ORIGIN      
        1 gcggtggggg ctggccgccg tgccctcgcc gatcagccgg accacggccc cctcgatcag
       61 cgatcgctcg tcgtcggtgg tggcggcgct ggcggccgca cggatcgcgg cctccagcag
      121 cgggcggaag gcggcgtggt cctcggacgt ggcgcccagt gaatgcgcca ccgagacgat
      181 ccgcgcaccg ctgccggcat cgaccgtgag cgccaccggc gtggccggaa tcctggcccc
      241 ggccgcgatc gccacaccca tggtccccgt ggcggccggc aggtgtcccg ggaagccggc
      301 cgcgggggtc agctggatca tcgacgaggc ggcgttcagg gtctcgacgg cattgccggg
      361 gccgctcgtc ttcaggacga ggttgcgatg gctctcgctg cgcttcgtga cgcgacggcc
      421 gttccgggtg acgatctcgc tcacggtcag gttgaaggtc gtcgtcgggt cggcggtgtc
      481 gtggtcgatc tcgaggaaga gatcgttgcc ccagatgccc ggatcggcga cggaggcctc
      541 gtcgacccgg cgcccggcgc tgaccgtgaa gacggtggcg ccggcgccgg gctgggcccg
      601 gagcgtggcg ctggcggcgg tcgccggggc ggtcgacgag gtggtgtcgg cgacccgcac
      661 ggcccagcat tcgctgccgc cattgatgaa gaactgctgg atcgcgtagc tggcctcgct
      721 ggtcgggctc agcccgccga gctcgcgttc caggtcggcc acgccgacga gccgcacggg
      781 cttgcccaga ggcccgcgcg cgaagcggtc gacgaaggcc gcgaccgagg tggcgacgcc
      841 ggtgatggtc cgcacgccgc tcggaatctc ctcgatatgg gcgcctggat aggtcggctc
      901 gaccggcatc ggggggaggc tcccgttctg tgcggtggtg ctaagggggg cgggcgaagg
      961 gcccggtgtt ggtgggaacg cgggccgctc acggtcgtta ccctcggccg aagctcaatg
     1021 gaagcccacg ttcatgacat agtcccgcag cagctcctcc tcgtgccggg tctcgtccag
     1081 gagggcctgc agcgcctcgc gcgcgttcag cacgcccagc gggcggcccg cgtcgtcggt
     1141 gatcgggagg ttcttgaagc cccgttcctt catggcctcc caggcctcct gcagccagcc
     1201 gtccggccgc acgctgacga catcccgcgt catcgcctgg gcgaccgggg cggtgcagct
     1261 cgccccggcg cagcgtccga tctgtcgcac cacgtccgtc ttggtcagaa taccggcgag
     1321 ccggccctcc tcatcgcgga ccagaagcat gtcctgaccg tcgtccagga gacgcgccgc
     1381 ctgcagcagg gcggcgccca actcgatgca ctgcatccgc ttgcgcgtcc ttggcagcag
     1441 cttctcgacc agcatgccgg gcgtcctccg tcgattcacg taatgccggg accggcccgc
     1501 atcagccggc cctgcgcaac ctcgccggca gcggccgctc gccatagtcg accgcgccgc
     1561 atgtgaggat cttcccgctt tcgtcggcga ccatgcgcac ccggcggctg cccgagaaga
     1621 aggtcagcct gatccgcccc gcgtcctcgc cctcgccgcg ctcgagccgg ctcgtgaccg
     1681 tgccctcgcg catccgccgc aggagctcgt ccgtgtcgat tcgcaaggcg ccggccagca
     1741 cctccgcgtc gatctggatc gtttcgtcgt catattcgat ttcagtcatc tttccactcc
     1801 acttgtcctg gtcaatgcgc cgaggcggca ccgatcggca atccggcgcc cggccaggcg
     1861 cagtcggcga tggtcgtctg atccagcgct tgcatgaagg cctcgcgggc cagggcgagc
     1921 tgcggcttca gacggcagct cggcgcaagc acgcaatggc cgccatcggc gcgaaagcac
     1981 tcgaccatcg cgaagcgctg ctcgagatgg cgcaccacct cgccaaccgt gatcgcctcc
     2041 gccggccgcg ccagctgcag cccgccatgg cggccgcgct ggctgccgac gaagctgccg
     2101 cggccgagag cccgcaccac cttggccaga tgatgcgccg agacctggag ctcatcggcg
     2161 atccgcccgg tcgaaagggc ggcacccggc gcggcggcga ggcgcatcag gacccgcaag
     2221 ccgaaatcgg tcgaagtcgc gaggcgcatc gctgatcctt atttggtatt gacgataccg
     2281 attatatcga acgataatca gcattgtaaa taccgattat gtgaggagga gcccgccatg
     2341 accccgacga cccaggcccc gcacgaccgg cccgttggcg agatcgccgc ccgactgccg
     2401 ggtgcgagcg gcgtctttcg ccagtacggc atcgatttct gctgccatgg cgacgtgccg
     2461 ctccagcagg cggcgacgag ccgtggcctc gacccgcgtg tcgtcctgga ggcgttgaac
     2521 gcgctcgacc ccgcggccgc gccgctggcg ccggaggaga ccggcgccct gatcgaccat
     2581 atccagaccc gctaccatga cgttcaccgg cggcagattc cggagctgat cgagctctcg
     2641 agcaaggtcg agctggtcca tctcacccat cccgacgtgc cgaccggcct cgccgacgct
     2701 ctcgagcgga tccttggcga gctcgaggtg cacatgaaga aggaggagct gatcctcttt
     2761 ccggccatgc gccggcaggc ggcggcgcgg ctcgccacgc cgatcggcga gatgcgccac
     2821 gatcacgaca gccacggccg cttcctcgag gagatggcgc ggctgaccga cgactgcgtc
     2881 ccgccccagg gcgcctgccg ttcctggcag gccctctacg ccggcgctgc gcagctccgc
     2941 gccgacctga tggagcacat ccatctggag aacaacgtcc tcttcccgcg cttcgaggct
     3001 gcctgagcgg gccgcggaag aaaacttcat gaaagctgcc tgaaaaggct ctagggatcg
     3061 aggcgtcggc ggaatcggtc cgtcggcgcc acattcgtgc caagggagag aatcgacatg
     3121 tccgagcaat ggcttccgag cctcgtcacg cccaccccgc aggaagggtt cgatctggcc
     3181 gtcaagctct cgcgcatggc ggtcaagctg acccagccgt cggccgaggt cagggaagcc
     3241 ctgcggggtg gctatgccca cgatgcgagc gacctgatcg ccgtctccca tgtcgtcgcc
     3301 actcacttcc agacgatcgc cgccgccaac ggctactggc ggaccgaccg cggctgacgc
     3361 gtcacccggc cgccccgacc cgggtggccg gcctcccttt ggccggctgt tcatcgcgcc
     3421 ggccacgggc tatggagggg caaggtcgcg atcgctcgaa tgtcgagccg aacagggaga
     3481 aaagccatga cccgcatcct ccaccccacc cgtcgccagc tcaccggggc catggccctg
     3541 ggtgccgccg gcctgaccct gccgctgggc ttcggccgcc gcgccagggc cgccgaccgg
     3601 cgcatcgcca tgatcgtcaa gaacctcggc aacagctatt tcgacgcctg cgccagtggt
     3661 ggcgccgagg cggcggccga gctcggcggc accgagctga tctacacggc ctcgaccaag
     3721 ccgacggccg aggaccagat cgcggtgatc gattcgctga tcgcccagcg ggtcgacggc
     3781 atcatcgtct cggccaacga cgcgaacgcc ctggtgccgg tcggcaagaa ggcgatgcag
     3841 cgtggcatca aggtcatgtc cttcgattcg gcgatcgccc ccgaaggccg gctcgtccac
     3901 ctctccgcct cctcgaccga cctgatcggc gccaagcagg tgcagatgat cgccaagacc
     3961 ctgaacaacg agggcgaggt ggcgatcctc tcggcggcct ccaccatgac caaccagaac
     4021 gcctggatcg aggcgatgaa ggtcgagtgg gcgaagccgg aatatgccaa catgccgctg
     4081 gtcgcgaccg tctacggcga tgaccaggac gacaagagct atcgcgagat gcaggccctg
     4141 gtgaaggcgc acccgaacct caagggcgtc atctcgccga ccacgatcgg catccgcgcc
     4201 ggggccaagg cgatcatgga cgagggtctc gtgggccagg tctacatcac cggcctcggc
     4261 ctgccgtcgg agatggaggc cgcggtcctg gccggcgcct gcgacagctt cgccatctgg
     4321 aacccggtcg actacggcta tgcggcgacc atgatcatgg gggccatcct cgacggcgcc
     4381 ggcaccgccg agggcgacac cctgcccatg ggccggatcg gcgagaccgt cgtcggtgcc
     4441 gaaggcgagg cggtgatggg cgagcccttc gtcttcgaca agaccaacat cgaggaatac
     4501 gctcagatct tctgagcctg catcgaacgg ccggcggcgc tcgcgtgccg ccggcccccc
     4561 tccgctcgcc cggcactgca gtcgagaccc atggcgcccg tcctctcgct cagccacatc
     4621 tccaagggct tccctggcgt ccaggcgctg gacgacgtct cgctcacgct ggagcgcggc
     4681 tccgtcacgg cgctgatcgg cgagaacggc gccggcaagt cgaccatcgt caagatcctg
     4741 accggcatct acacgcccga tgccggcacc gtgaacgtcg aggggaggga ccgccgcttc
     4801 gccaccccca aggacgcctg ggccgccggc atcgccgcca tccatcagga gacggtgatg
     4861 ttcgacgagc tgtcggtcca ggagaacatc ttcatgggcc acatgccggg cggccggctg
     4921 atcgaccgga cggccatccg cgaccggacc aaagagctgc tcgcccgcat cgacgcggat
     4981 ttcgcccccg agaccccgct gaagcggctc tccgtggcgc aaaagcacat ggtcgagatc
     5041 gcccgtgccc tgtcgcacga ggcggccgtg atcatcatgg acgagcccac cgcctcgctc
     5101 tcccggcacg agatcggcga gctcttctcc ctcgtcgacc agctccggga cgagggcagg
     5161 gcgatcctct tcatcagcca caagttcgac gagatcttcc gcgtttcaga ccgctgggtc
     5221 tgcctgcgcg atggccggaa ggtcggcgag ggcctgaccg ccgaggtgac cgagcccgag
     5281 ctcgtccgcc tcatggtcgg ccggccgatc gaccaggtct tccccaagcg cgatgtcgtc
     5341 atcggcgagg tggtgctcga ggtcgaaggc ctcagcaacg ccaccgagtt cgccgatatc
     5401 tccttcgagc tccgccgggg cgagatcctc ggcctctacg gcctcgtcgg cgccggccgc
     5461 tccgaggcca tgcagtgcgt cttcggcctc tcgcagccga cccgcgggcg gatcgtcatg
     5521 gagggccgcc ccctggcacc gagggcgccc ggggatgcca tcgacgctgg catctcctac
     5581 gtgcccgagg atcgccagga gcagggtgtg gtgctgccgc tcggcatccg cgccaacatc
     5641 acgctcgcct cgctcgcccg ccacgcccgg ggctggttcc tctcctcgag ctcggagctg
     5701 gacgcgaccc gggcactcgg cgaccggctc gcggtcaagg cggcgagctg ggagcagccg
     5761 gtgatggagc tctccggcgg caaccagcag aaggtggtga tcgccaagtg gctcgccacg
     5821 caaccccgcg tcatcatcct cgacgagccg accaagggca tcgacatcgg ctccaaggcc
     5881 gccgtccacg ccttcatcgg cgagctggtc gccgacggcc tcgcggtcgt gctgatcagc
     5941 tccgagctgc ccgaggtgat gggcctcgcc gaccgcatcc tcgtcatgca cgagggccgc
     6001 atcgtcgccc gcttcgcccg cgacgcctgg tcggccgagg cgatcgtcgc cgccgccacc
     6061 ggcgccgtgg cagcggaccg cgccgcatga gctggctcgc cgcccatcgg gaagtggtgc
     6121 tggccctggt catcgccctc atggtggcgg cgatcggcgc ctacgccccg gccttcgtcg
     6181 agggcacgag cctcttgggc gtgctcgacg acacggccct cctcttcatg ctcgcgctgg
     6241 gccagatggt ggtcatcctg acccgcggca tcgacctctc gatggccgcc aacctggcgc
     6301 tgaccggcat gctcgtggcc ctcgtcggcc agcacggccc tgacctcccg gcgctcgcct
     6361 acatcgtcct ggcgatcctg atcgggctcg gcttcggcct cggcaacggt gtcctgatcg
     6421 gcgggctcgg cattccgccc atcgtcgtca cgctcggcac gctctcgatc ttccggggca
     6481 tgatcgtcgt catcggcggc ggcgaccagg tcaatgccag cgagatgggc cggaccttcc
     6541 aggccttccc gcacaaggcg ctgctcgggc tctccagcgt cttctggatc gcgcttgctg
     6601 tcgccatcct gatgcagcta ttcctttccc gcacgcgcac cggccgcggc atctacgccg
     6661 tcggcggcaa cccgacggcc gcccgctatt gcggcctcgt gctcgagcgc taccaggtcc
     6721 tggtctacgc catcgccggc ggcatcgccg ggctctcggg ctatctctgg gtcgcccgct
     6781 acggcgtcgc ctattccgag atcgcgagcg gcttcgagct caccatcatc gcctcttgcg
     6841 tcatcggcgg cgtgtcgatc ggcggcggcg tcggcaccgt ccccggcacc ctgctcggcg
     6901 ccctcttcat cggcgtcatc gtcaatgcgc tgccgaccat gcaggtctcg cccttctggc
     6961 agatggccat ctccggcgcg gctatcctcg ccgccgtcat catcaacgcc cgcgccgagg
     7021 cccatcccga caagctgatc ctgcccgagg cgcgccgggc cgcggccggc gcacggggct
     7081 gaagccgttg cccgacagcc cgcgctacac cgtcgccgac cggccgccct tccgcctcgc
     7141 cgatctcctg ctccgctggg agagcatcct cgtcgtcctg ctggcgctgg tcttcgccgt
     7201 caatgcctgg gcgaccccct ggtttctcga catctacaac ctcgccgacg cgacctacaa
     7261 tttttccgag aaggcgatca tcgcgcttgc catggcgctg ctcatcctga cccgcgagat
     7321 cgacctctcg gtcgccgcca tcgtggcgct ggcggccctc ggcgtcggtg cggcgggagc
     7381 gctcggccag ccgacccccg tcctggttct gaccgggctc ggcatcggcc tcctctgcgg
     7441 cctctgcaac ggcgtgctgg tgacccgctt cggcgtgcat tcgatcgtca tcaccatcgg
     7501 cacgatgtcg ctctatcgcg gcatcgccca ggtttcgctc ggcgatcagg cgctcaccgc
     7561 cttcccgcag cccttcctcg acctgcagca gacctatttc ctggccgacc cgccgacccc
     7621 gctctccttc gtgctcttcc tcgctttggc ggtcgtcttc ggcatcgtcc tgcatctgac
     7681 cgccaccggg cgccggctct tcgccatcgg cgccaatccc gtggccgccc ggttctccgg
     7741 catccgggtc gatcgcatcc gcttcttgct cttcaccgtc tccggcctca tggccggcct
     7801 cgccggcgtg ctgctcaccg cccgcatcgg cgtcgtccgg cccaatatcg ccctgggctg
     7861 ggagctcgac gtggtcacca tggtgatcct cggcggggtg gccatctccg gcggcatcgg
     7921 ctcgatcccg ggcgtcgtgc tcgccgtgct ggtcctcggc ttcgccacct tcggcctgac
     7981 cctcctcaac gtcccgggca tcgtcatcaa cgtgctgctg ggctgcctcc tgatcggctc
     8041 gatcgccctg ccgatcctga tccgccgggc catgcgccgt cgccgcagca cctgagcccc
     8101 gatgccgcca gcggcaccgg ggccggcgac gaggtcaatc gccgggtgcc gacatgtgct
     8161 tggcgatata ggcgccctgg cccatccgct cgagcaggtc gagctgcagc tcgagccagg
     8221 ccgaatggcc ctcctcgtcg agcagggtct gctcgaagag cgtgcgtgtg ccgatatcgc
     8281 tctcctcggc cgccgcgtcg gccgcctcgg tgtagaaggc gatagcaccc tgctcctcgg
     8341 cgaggtcggc cttgaacatc tcctcgagcg acgtggcacg gcgcggcgtc ttgcccgcct
     8401 cgatcctggg gtcgcccttg aggaacagga tccggtcgat gaaggccccg gcatggccga
     8461 gctcctcctg catctcctcg cgcatcttcg ccgccagccg gtcgaggccc caatcgtcca
     8521 gcacatgcgc gtgcatgagg tactgatgca ccgcggtcaa ctccatcgag agcgcctgct
     8581 ggaggttctc gagcgccttc gtgttcttgc tcatggcctt gcctttcccg gtcgcttgaa
     8641 tctgttcgcc agtcgggcgg gccggcggcc cggaaacgac accctggcca cctgctcgcc
     8701 cccctgtatg gtgggcaagc ccgtggccat ccgctaccct ttgcgacggc gtgtcacgca
     8761 tcgggggaac acttgagcaa tccagaattg ggccggggcg ggccgctgct cgtcggcgtc
     8821 gatgccggca ccaccaacac caaggccatc atcatcgacc tcgccggctg cgtcgtcgcc
     8881 gaggcgagcg agcccacgcg aatcgagcat ccgcggccgg aatgggccga gtacgcggcc
     8941 gaaacgctct gggaggtggc ggccagcgcc attcgccggg ccgtcaacca gctcgacgac
     9001 cccgaccgca tccagggtgt cgccgtcgcc agcatggccg agaccggcgt gccgctcgac
     9061 ggctccggcg cagccaccgg ccccgccatc gcctggttcg acaagcgcgc ccgcgccgag
     9121 ctggcgctga tcgaggagcg cgtcggcccg gatcgcctct tcgagatctc cggcctcgcc
     9181 cccgagccca tcttcggcct ctgcaagctc ctctggcaca agcaccaccg cccggacagc
     9241 ttcgcccgca cggtccagtg gctgaacgcc gccgactatc tcgcctggcg cctctcgggc
     9301 gagatggcga ccgactacag cctcgccagc cgcaccttcg ccctcgatct cggcacgcag
     9361 cgatggtcga cggaggtgct ggaggcggtc ggcgtcgacg catccctgat ggcgcccctg
     9421 gtgcgcagcg gccaaagggt cggcaaggtg caggcggaag cggcccgcgc caccggcctg
     9481 ccccgccatt gcgtcgtggc ggccggcggc cacgaccaca tggtcggctc ggtcgccgcc
     9541 gacgccatgc ggcccggcgt catgctgctc tcgaccggca ccaccgaggc gatcctgatg
     9601 ggccgcgaca caccgggaaa ggaccccgcc ctcggccggg tcggctatgc ccagggtctg
     9661 atgtgggtcg acgagcccgt ctactatgtc atcggcgggg tcttcaccgc cggcggcgcg
     9721 atcgagtggt tccgccgcac gctgggtgcc ggggcggggg ccgagggtgc cgactacgcc
     9781 accctcgtcg ccgaggccgc cgccgccccg cccggcagcc tcggcgccct cttcctgccg
     9841 cagctccgcc tcggcacccc gccgcatatc gacggccacg cgcggggcgc ctttctcggc
     9901 ctcagcaccg acgtcaccca cggctgcctc tttcgcgccg tgctcgaggg catcgccgcg
     9961 gattcgcgcc tctgcgtcga aggcatgctg cggctcctgc ctgccgaccc gccgcgcgag
    10021 gtccggatca tcggcgggct gacccgcaac cccctctaca tgtcgatcag gagcgccgtc
    10081 gccggtcgcc cgctcagcgt cgtcgacctg cccgacggtg tcgccgccgg tgcagcactc
    10141 ctcgccggcc tcggagccgg gctctacccg accctcgagg cgagcctgga ggcgatgcgc
    10201 ccgcccgagc gcgaggtcac gccggacgcg gcgatggccg ggctctacga ccgcctcgtg
    10261 cgcgaggtct acagcaaggc ctacccgacc gtgcgcgccc tgcacgaggc ggcgcagaag
    10321 gctatttcag caccgcctcg gtgacctcga ggtcctgctc gagcgcccgg accggcttga
    10381 tcttcgccct ggccagctcg ccgtgatagg cgcgcaccgc ctcctcgggg ccgacctcgc
    10441 cctcgaccag ccggcgcatc agggccacca gcgccagggg atgctcggcc aggttgatct
    10501 tgcggccgaa gagcacgacc cgcgccccgc ccttctgcga ggccttcagg agctcgaacg
    10561 tgtcgcgcgt cgtccccgcc ccgccgccga gaatgccgac gatcagggtc gggtcgtaat
    10621 tgaccagctc ctcgagcgcc ttcggcccgt tgtagacgat cttcaggaac tcgggccgct
    10681 gggcgtcggg caccgccgcg aagacgcgga cgatgttgtc gttgacgaac tcgccgatct
    10741 cgaccgccgt cgccttgtcc atgccgacat tcgggttgaa gacctcgagg aagtgcttga
    10801 agccgtgctc ctcgcactcg tgccggaaag cccggtactc cgcacccgcg gcggcgtcga
    10861 tcgccgcgtc gttcataaag gtgatcgagt agaggccgag atcgcagagt gctttggcat
    10921 gcgccaccga agccgtccga tgcggcagcg acggctgctg cttgtagcgg ctgtgccggg
    10981 caccccagat gtcggtcgtg tcgttggccc ggatagcggt gccgacccgg ctcttctcga
    11041 acacgcccct gcgcttcaga tggtcgagat tgccgaccga gagcagcacc atgtcgagaa
    11101 tatcctgctc gaccagcgcc tccacctgcg ccagatgatc gaagcgcgac ttgaatcgcc
    11161 ccgtcgacct gcccttcgca tcccgcgcca cgcccggcat cggaatgccg ccacccatgt
    11221 cgccgtcctt ggcgtcacag atgagaaaat ccttcgcacc cgaggggtcc ttccggattc
    11281 gagccagttt cttctcgagc cgcgtcctcg gcatcgcgtt ccccaggtca tgtggtgcca
    11341 tggcgcaaaa acgcccggca agccgccggg cgccgcctgc ttaggcgttg cgcggcgggc
    11401 tgacaagatg gacaggcccg gcagctgccg agccaaggcc atctcgtcca gacagtcacc
    11461 gtattcagcc catttgagaa gatacgagga cactttacga ccgcatgaag cgcgtcggcg
    11521 gcggcgcggc accccggctt catcagcgat ccacgctgct gtatgacagc cgcctacccg
    11581 cgcccgccgc ctcgacccag cccaccgctg ccgcccagac cactaccgaa cccgccgcga
    11641 ctgccgcctg ctccgcggcc acgggccggc aggtgcggcg gccgcagcgg cggtcgggca
    11701 tcgggacggt tgtccgagtt gagtcctaag ggtgtcagga tgcccgacga ccgcagatca
    11761 cgacgcagct ctggaatgag ggtcccgaag cctcgcggcg accggcctca gtacacctcc
    11821 ggcacgtaga gcgcgtccgg caggaccttg cgttcgtagt ccgggttgaa gacccggtcg
    11881 ggcaggttga tcgggtcgtg cggcacgtcg ccgtagggca cctgccggag gaggtggtgg
    11941 atgcagttga gccgggcctg cttcttgtcg ttgccggcga cgatcgacca gggcgcctcc
    12001 ggtatgttgg tgcgctccag catctcctcc ttggccttgg tgtaggcctc ccagcggact
    12061 cgtgattcga ggtccatcgg gctcagcttc cactgcttca tggggtcgtg gatgcgcatg
    12121 aggaagcgga gctgctgctc ctggtcggtg atcgagaacc agtacttgac cagcttgatg
    12181 cccgagcgga ccagcatgcg ctcgaactcg ggcacgtcgg tgaagaattc ctggacctgc
    12241 gcctcgctgg cgaagcccat cacccgctcg accccggcgc ggttgtacca ggaacggtcg
    12301 aagaggacga tctcgccgcc ggccggcaga tgcggcacgt agcgctggaa ataccactgc
    12361 gtccgctcgc gctcggtcgg cgccggcagg gcgacgacac ggcagacccg ggggttgagc
    12421 cgctgcgtga tgcgcttgat caccccgccc ttgccggccg aatcgcgccc ctcgaacagc
    12481 accacgagct tgtagccggt atgcgccacc cagtcctgca gcctgatcag ctccgcctgc
    12541 agccggaaga gctcgaggaa ataggtgcgc cgctcgacgc tcgacttgtg ctcgtggtcg
    12601 gtgatcgagc gcagctcgcg ggcgatccgc tcgtcgtcga tctccatctc gagctcttcg
    12661 tcgaagctgt ccatgagctc cgcctcgacc cagcttttct ctcgcccgct ctggcgctcg
    12721 tcggtcatgg atcctctccg tcagctgcaa tggtccggtc ggcccgaggg gtggccgctg
    12781 tcgcgtcggc gtttttccgc gaggctcgcg gcgccgcctc gagccggacg ccgctgatgc
    12841 gacccatgca acagacggct ccccggggcc cggatcgtcg cgtatggtgc atcattgctg
    12901 tgacgactgg tgcatcgggc gcgacattcg ccgtcggcat gttgcatatc ctgcagcatc
    12961 ccgtgacggg cgaatccgcc gggctggcga tcgaacccgg gcgcgagcca acgagcccgc
    13021 ggccccgcca gcctttggga atgaaggtga tcttgcctct cgccgaggcc gcgccgccgt
    13081 aagaaccaac agaagatcga gaaatgccga acgagccggc gccggccgcg ctcggcagct
    13141 cgcctgtgct gggaacatcg ccagaaaacg ctccctgacg cccggtcggc tgtcggcagg
    13201 ggcgttgatc cgcgcgccga tgcaccattc ggccccgttt ccgtcaggag cgctcgagca
    13261 cgccgagcgc gtggagcgcg atcatgcgtt cctcgtcggt cggcacgacg cgcacctcga
    13321 ccgcgctctt cgcggtggag acgatcgtgg ctccctcggc atttgccgcc tcgtcgagct
    13381 cgatgccgag ccaggcgagg ccccggcaga tcctggcccg gacgccatga ccgtgctcgc
    13441 cgatgccggc cgtgaagacg aggccgtcga gcccttcgag cgctgccgct tgggcagcga
    13501 tctggcacct ggcccggtag gcgaaatagg cgatcgcctc ctcggcttcc ggccgatcgc
    13561 tggcttcgag gtcgcgcatg tcgctgctga tgcccgagag ccccttgagg ccggactcgt
    13621 ggtagagcag ggtctggagc cgagcggtgt cgaagccccg ctcgtcgatc agatggagca
    13681 ggacgccggg gtcgatctgg cccgtgcggg tgcccatcgg cagcccgtcg agggcggtga
    13741 agcccatgga gctgtcgatg cttcttccgg ccagcatggc gcagcagctc gccccgttgc
    13801 cgagatgcgc cacgaccacc cgcccggcgg cgagggccgg tgccgtccgc gccagctcgc
    13861 gggcgatgta ttcgtaggac aggccgtgga agccgtagcg gcgcaggccc tcgtcgtaga
    13921 agtgccgcgg cagcgcgaag gtatcggcga cccaggggtg gccgcgatgg aaggccgtgt
    13981 cgaagcaggc gacctggggc acgcccggat aggccgccgc cacggcctcg atggcgccga
    14041 gattgtgcgg ctggtgcagg ggtgccagcg gcaccagctt ccgcagctcc gccatcaccc
    14101 ggtcgtccac cagcaccggt gccgcatgct cggtgccgcc atgcacgacc cgatggccga
    14161 cggcggcgat gccgcggccg ccgaaccgct cgccgagcca ctcgatcagc agtcggatgg
    14221 cgccgtcgtg atccaggacg gccatgtcgg tgacccgggc gagctcttcg cccgatccgt
    14281 cctcggcgac gaagcgcggc ctgacgccga tgccatcgat ctgcccgctg gcgaggaccg
    14341 cggccccgct ggcggcggtg gcaggctcga agaccctgaa cttcaggctc gaggagcccg
    14401 cgttgatcac caggacgaga cccgtgcgct cggtcattgc cgccttctcc atcatggctc
    14461 agcccatcac gtgatagccg ccgtcgacat agatcgtgtt gccggtgatc agctggcctg
    14521 ccggcgtggc cagggctgcc gtcgccgcac ccacgtccct gatgctgacg agccggttgc
    14581 tcggcgcccg cgtctcggcc gcattcagca gctcctcgaa ctcgttgatc cccgaggccg
    14641 cgcgcgtgcg gatggggccg ggcgagatgg cgtggacgcg gatccgccgc gggcccagct
    14701 cgtgcgccag atagcgcacg ctgcattcga gcgccgcctt gaccggcccc atcacgttgt
    14761 agtgcggcac caccttctcc gccccgtaat agctcatggc gaaaagcgtg ccgccattcg
    14821 tcatcagcgg ggcggcgcag cgcgccatgc ggacgaagga atggcacgag atgtccatcg
    14881 cccggccgaa gccggggccg gaggagtcga gcaggccgcc ctgcagatcc tccttcggcg
    14941 cgaaggcgat cgagtggagc gcgaagtcga tgctgcccca gcgcttcccg gccgcctcga
    15001 acaccgcgtc gagctcgccc tcgcgctcga cattgcaggg catgaggatc ggcgcctcga
    15061 gctcctcggc gattggctgg acatgcggcc tggccttctc gttgagatag gtcagcgcca
    15121 gctcggcgcc gaggaagcgg aaggcagccg cgcagccata ggcgatcgac tgccggttgg
    15181 cgacaccgag caccacgccc cgcttgccct cgagggtccg cgcctgtggg tcgaaggtca
    15241 ggatctcgct gtcgctcatg ctcgctacct ctcggattcg atgggcctgc accttgaaat
    15301 gacgggcgcg gaccggaaga tcgccggttc ttgcgcgact gccgtgacag tgtcgatggc
    15361 gcgaattggc tcagcccctg ccgccgcagc cggcgtcgtg accggctccg gcaaggcgaa
    15421 gcgattgtcg gccggatcgg gcggcagcgt gcgcgagccg aacgacggca gccgatagtc
    15481 cctcggcgcc gccctgcggt cgcgcgcggc gcaccgccgg acaagcaggc acccacgacg
    15541 cgggcatcgg ccatcgcctg cccggccagg gagcgacggc cggtttgcag cgccgaccat
    15601 ggacgacccg gggacgccat gcgccgctca ccggccgaca cggcgggctg ccggcggctg
    15661 gcgagggtcg cgatcgagag tgcccgccag tgctcggtgc cggccggcaa atcaaccgac
    15721 cggcatggcc aattcgccgg ttgccttggc accaggggtt cgccgtgggg ccggccgagc
    15781 ttcaacgggc ccgagcggcg ttgcggctgg ctcgacagat ggctcatggg cgccggtcgc
    15841 cggcgtcgcg agatgcgcga gccggggcag gagcccggcc agcgtccggc agaccaccgg
    15901 gtcataacgg tcgtgctgtt ccagcagatg gcggaccacc gtctccgccg attcggcctc
    15961 tcgataggag cgtgggcgga tgcgcgcgca gaagacgtcc gcggccgcca ggatgcgacc
    16021 cggcagggaa atttcggccg cgaagcggcc atgcggatag cccgtgccgt cgagccgctc
    16081 gtgcatctcg gcaagcacgt cagcgaccgg cagggcgaag tcgatgctgc cgatgatcct
    16141 gagcgtgtgg tcgatatggg tgcgcatcag ggccatgtcg gcctccgtgt ggcgctcggg
    16201 cttggccagg agcgagcgcg gcaggaagac cttgccgagc tggctgagct cggcggcacg
    16261 gccgagcgtc gcgcgctcgt cggccccgag gtcgagcacc gtcccgatgg cctgcgccac
    16321 ctcgcgcacc gcttccgcgt ggccgacgag ataggggtcg accgtgccga tcgcccgcat
    16381 gaaggcgtgg caggtctggc cggccagctc ctcgcgcaac cgtcgctcca gggcgcgctc
    16441 ggtgacgtcg cggccgatga gcaccgtgcc ctcgatgccc acgctcgcgc cgggcaccgg
    16501 cgcctgggtc acctcgagca gcaacggccg ggccggatcg ccggtctcga gctcgagcga
    16561 cggcgcggca ccaccggccg ccgcgtcctc ggcattggcc ctgagcgcct cggccaccgc
    16621 ggccggaaaa agctcctcgt cggtccggcc gatcaccgcc gccgccggca tcgccagatg
    16681 gcgggcgaag gtgggattga cgaaccggta atgtccctgc gcgtccttga ggccgacgag
    16741 ctcggcggcg tgatccgtga tggtggtcag gatgcggttc gcggcggcca ggcgttcggc
    16801 cagcgcccgg tactgctcgg ccaggagccg acggtggcgg gcttcgcgca gccaccagag
    16861 ggcgcagcag gcaagtgcca cggcgagcga gatggcaacc ccgatcagcc gcgcggtgcg
    16921 ctggaactcg gccagggtgg cgaaggcgct cgccttgtcg atcgattgca cgacgatcca
    16981 gtcggtgccg ggtactgaag cggtggcgac gaggtccatc gaccatcccg gtgtgggcag
    17041 gctgccgccg aacggaatcg cggtccaacg ttccccggca tcgcttcggg cgagcatggt
    17101 gctgcctgac tgcagctcga cgaccagacg gctttcctca ccctcgagat cggtgggccg
    17161 atgctcgagg agggccgcca ggcgagcggt ggcgtcgatg gtgaccgcga gggcgccgac
    17221 cggcgctgct gttgcctctt cggcgaggtc cagcggtggt gcgatcggct gcacgagatc
    17281 gagcaggagg ctttcgccct gctggcgcag ctggcgatag ccggcgccgg caaccggaat
    17341 ggcgcgaggc tcgtcgctcg tggccgcttg ccggccgttg cggtcggcga ccacggtgct
    17401 gccatccgcg ccgagcagtt gcgccgcctc cagtccctgc tgatcgacga acgagtcgag
    17461 cacctgccgc atgtaggggc gctgctcgag caaggcggcg gcgagccgcg ggtcgtcggc
    17521 ggcgtggacc tgctcggcga ggtagagccg gatggtcggc gaggcggcga gacggtcggc
    17581 gcggccgaca tggccagcga gccaggtgtc gaggctttcg gcacggctgg aagccagggc
    17641 cgcgagccgt gcctcgacca tgccctgcag caccgtcgcc cggctccgca ccagaagctc
    17701 gacgaggccg aggcccaggc cgagcgtgcc ggcgacgagc acggccgaga tgaccaggaa
    17761 gcgccataga cgaggcgggg tcgcgcgaat gtcgggagcg atggatgcgg gctcggtgga
    17821 aggctgggac atggtgtcga tcggccgatc ctgtcgggtt gatggctggc ggtgggccgg
    17881 agcctcagcg gctggcggga gcggtggtgc tgctccggtc cacggcggag agcatcaggc
    17941 ggctgctctc cacgtgcgcc catcgggccg tctcgttgac cgagtagtag ggcgcgtagt
    18001 gggccacgcg ctcatgcggc agcacggcgt tggtgaggtt gtcgagaatg acggcggtgt
    18061 cgtcgaggta gaccgcgaga accgcgtggg cgagctggcg gagctcgtcc tggacgacca
    18121 cgaggcgcag ccgcgccgcc ggcacgccca gctgccgcag cgacacgtac ttggcgatcg
    18181 cgtagtcctc gcagtcgccg gagcgcgcca ggaactcgag cggcgacgcc cagtagtcgg
    18241 acttgccgtg attggcggca tcggaacggt agcgccactg gttgacgaag cggttgacct
    18301 tgctgacctg ctccggcagc ggggcaccac gcagcccctg cagcaatgcc tgccaggcca
    18361 tcacgccgcg attgggacag gccgtcggat ccagcctgca ggcatcgtag gtcggctgct
    18421 ccagggccga gcgctccagc acctggcgcc acttgggcag cgcggcgagc gattcggcgc
    18481 ggaactcggc cgttccgaac agtgctaccg gcaccgttgc ctcggccgcg gcgatcggta
    18541 gcagtgtcat cacgatggcc aggagccagc gcggcatggc atttcctccg acagggacgg
    18601 gtgcctttca ggcccgccgt ttctgctccg agattgccat gccttggtga atactccctt
    18661 gaagccggcg gatttccgcc gctcatcgga cgctcccgcg tacgccaccg cctgcttgcc
    18721 gaagccgcat cacgtagccg atgatctcgg ccaccgcctg gtagtgctgc tgcgggatca
    18781 gcgccccgag ctcgacggac cgatgcagtg cccgggcgag gggcgggctc tcgatgatcg
    18841 gcacgtcgtg ctcctgcgcc agctcgcgga tgcgcagggc caggctgtcg acgcccttgg
    18901 cgaccagctt gggcgccggc gtctcgcccg gctcgtagcg gagcgcgacg gcgaaatggg
    18961 tcgggttggt gatcaccacc gtgctggtcg gcacctcggc catcatccgc cgccgcgcct
    19021 tctccatgcg cagcgatcgc agccgctgct tgacgaccgg gtcgccgtcg ctctgcttgt
    19081 gctcgtcctg cagatcgcgc cgcgacatgc gcatctgctt catgaactcg aagcgctgat
    19141 aggcgtagtc gaggccggcc acgagcacga ggcaggcggc gatggccccg ctgacccgga
    19201 tcgccatctc ccagagcacg accagcgccg cagcgatctc gagctggccc gaagccacga
    19261 gccgctgccg gtccggccag accacgacga cgccgatgac cgtcatcagg ccgaccttga
    19321 cgagaccctt gatgaactcg accagcgact tgagcgagaa gagacgcttg aacccggcga
    19381 tcggcgagat ccgctcggcc ttgggcatca tgctctgggc ggtccagact agggcaccct
    19441 gcagggccgg tgccgccacc gacgcgacga tcgtgaccag gagcggcacc agcagggcca
    19501 ggcccatcgc tgccagcgcc cggcgcaggc tgccgccgag cgccgcagcg cccagggtcg
    19561 ggtcgccggc gtggcgcagg tagtggctca gcgccagggc cagatcgcgc atggcacccg
    19621 gcagggcgat ggccagcacg agggtcaggg agccgatgag caggaggttc gatacctcgc
    19681 gcgagctcgc ggtctggccc ttctcccggg catcgtcgag ccgcttctgc gacgggtcct
    19741 cggttttctg ggagtcgtcc tgctcctcgg ccatgctcct agaccggctc gagccaggtc
    19801 atcgtcgtgc gcagcagggt cgtcgccagc ccgaccacgg tgccgagcga cagcatgaag
    19861 agcccgaagg ccaggacgat ctgcagcggc atggcgacga acagcacctg gaaggacggc
    19921 atcaggcgat tgagcacgcc gagcaggaga tagagcagca ggctgaagag cacgacgggt
    19981 gccgccatcc gaaggccggt ctcgatcgcc tcgccgccga gccggacgag cacctcgacg
    20041 aggccggcga gatcgaggct gccggtcgtc tcgagcacgc tgaagctcgt ggtcagcgcc
    20101 tgcaggacga gcaggtgcgc gtcgctgacg acgatgacga cgagggccag gatggccagg
    20161 aaattgccgg tcacggttcc ctgggtcgcc tcctgcgggt cgaagaacgc ggccgtggcg
    20221 aggcccgact gcatggcgac gagggagccg ccgaaatgga tggcgaacat gacgagccgg
    20281 atgatggcgc cgatcaggag gccggtgagc acctcggcga tcaccagcag cgtcagaccc
    20341 gccacatccg tcggcaggac cagccggccg ctcatcggca gcccgacaag cagggcgatg
    20401 gcaagcgcga tgcccagccg gacgcgcgag acgacatagg gctcgccgaa ggccggcagg
    20461 accatgaggg cggcaccgac ccggcagaag acgaggccga tgaccgtgat ctcgcgggcg
    20521 aggagcaggt cgagcgggtt catgggacgt gccgccgccc ggcctgccgc ccgccggctg
    20581 cctgctgctc agccggcatc gacgccggcg attcgcagga acagcgtctg ggtgaactcc
    20641 accagggtcg ccagcatgaa gggtgtcgcg accatcaggg tcatgaacat ggcga
//
LOCUS       DAOTFI010000063        20507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3139_length_20507_cov_6.977557, whole genome shotgun sequence.
ACCESSION   DAOTFI010000063 DAOTFI010000000
VERSION     DAOTFI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 20507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20507
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3139_length_20507_cov_6.977557"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="P5558_08245"
     CDS             complement(<1..762)
                     /locus_tag="P5558_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRY24338.1"
                     /translation="MMADRRILLAGIQHETNGFSNLRTTLADFRSYQYAEGSAIIERY
                     AGSGTEIGGMIRGAAAAGLELVPTLFTFAMPAGLVEAGAFEKLLGVIEHHARPLAPFD
                     GALVVLHGAMDVAGREDADAGVVRFVRGFLAPNAPVVATLDYHANISQGLVDAADVLV
                     GYDTFPHSDFFERGEEAASILGRLLDGMERPAKAFRKLPLLPATVKQATTAPPMARIL
                     AAAHAAEGRPGVLTCTVAAGYTYGDVPHLGAAVLAY"
     gene            832..969
                     /locus_tag="P5558_08250"
     CDS             832..969
                     /locus_tag="P5558_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24339.1"
                     /translation="MTGLSSGSGRVSGAEVSFRPPLIAGSVIVTYVPMVVLWLPRVHG
                     F"
     gene            1004..1771
                     /locus_tag="P5558_08255"
     CDS             1004..1771
                     /locus_tag="P5558_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY24340.1"
                     /translation="MMGKIRPNRAKEKLAAGGIVVSLGGLASSDLIDQIGPMGLDAVW
                     LEAEHGPVDYGHIGDLTRACDLWGMTSIVRVNSLDYGTIYRVLDLGAQAICVPHVDTA
                     NDAKGFVEAAKFAPIGRRGLFTGRQGFGVPDYLEVANDQTMLIALIEDAKAVENLDAI
                     LAVDHIDVFFVAPSDLAASMGHIGKIQHPEVQGTIDRTLQRIAAAGRTPGTLLLGHQN
                     VARYTALGVRCFMTPVLPLIAEGVGSVLEAAAASGRR"
     gene            complement(1781..2548)
                     /locus_tag="P5558_08260"
     CDS             complement(1781..2548)
                     /locus_tag="P5558_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY24341.1"
                     /translation="MTTNALKRRVLAGEPVYGAWLGLADAAVAELMAHAGYDFLILDQ
                     EHGPGSLETAADVMRAAEAAGCPLVVRVPWNDQVYLKRILDLGATSVMIPLLEGAEAA
                     KAAVAACRYPPHGTRGYAAPSHRCTRWGKDANYLAEWNDRLMILGQLESARAVDEAAA
                     IAAVDGIDMPFIGINDMAGSIGRLGQLDHPEVRELVERCERNLRPSGKPLGTVPSAMR
                     TVPELFAAGYTMVAGAVDSMLLRKAALADLEAHRPGA"
     gene            complement(2634..2993)
                     /locus_tag="P5558_08265"
     CDS             complement(2634..2993)
                     /locus_tag="P5558_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24342.1"
                     /translation="MRSLGFGLALLLAAHAAKAGEPVVEGVEARPEAAGTWRFDVTVR
                     HGDEGWDHYADKWVVLTPDGQVLGERVLLHPHEDEQPFTRSLGGVAIPPGTDEVLVQA
                     EDSVHGPGPTLAVRLGP"
     gene            complement(2993..4939)
                     /locus_tag="P5558_08270"
     CDS             complement(2993..4939)
                     /locus_tag="P5558_08270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRY24343.1"
                     /translation="MQERPFGGWPSPLSAAKATGAQNRLGQIVLDGERVLWLEGRPAE
                     GGRVSLMALDRPGGGRQATELTPAPLNVRTRLHEYGGSCVAARDGLVVLVNDRDQRLY
                     RRDADGSIRPISPDTGGSLRFAEPLIDWQRQRVVAVAEDHRTGGEPANLLVALPLAGG
                     EPVILAESHDFFACPRLSSDGRRLAWLVWDHPDMPWDATRLMTARLASDGTPTDVSCV
                     AGAGGGEAVLQPEFLPDGRLLYLSDRTGFWNLWQEGGEAPLLERDSDFGGPLWQVGNR
                     WWTPIDARRCLVAWGEDGLGRLGILHLEDGRLATMDLPFADFTEIHAAGGRAVFRGSA
                     LDRTAEIAALDLESRKLEILCAAGPLELDPAWISAPRPISFPSRNGRTAHAFHYPPTN
                     PDFRAPEGEKPPLIVRSHGGPTSATSPALRLGYQFWTSRGFAIVDVDYAGSTGYGRAY
                     RDSLRGQWGIADVEDCIAAAEHLVAEGLADPDRLVISGGSAGGFTTLAALTFHDTFKA
                     GASHYGIGDLEALMRTTHKFEARYLDRLIGPWPESAEVYRERSPIHHTDRLSCPLILF
                     QGLEDKVVTPDQAWAMRDALRAKKLPVACLEFEGEGHGFRSADVQRTVLLAEYAFYCR
                     IFGIVPAEPLPPIEIENLDCRGNQ"
     gene            complement(4957..5793)
                     /gene="sseA"
                     /locus_tag="P5558_08275"
     CDS             complement(4957..5793)
                     /gene="sseA"
                     /locus_tag="P5558_08275"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HRY24344.1"
                     /translation="MTDPLVSTDWLAERLEAPDIRLVDCTWFMPAEGRDPKAEHTAAH
                     LPGAVFFDIDEISESDTDLPHMLPSPAKFSARVRKLGLGDGVRIVVYDNNRFYASARV
                     WWMFRVMGHEDIAVLDGGLAKWRAEGRPVDDVPVRPTERHFTPRLNNFLVRDLEQMRA
                     NLTSRREQVVDTRSAARFTGSVAEPREGLRAGHIPGSRNLPASDLVTAEGTLKDAQAL
                     TALFAAAGVDLDQKLTTSCGSGVSAAVASLALARLGRPDVALYDGSWTEWGSRPDTPV
                     AR"
     gene            complement(5817..6827)
                     /locus_tag="P5558_08280"
     CDS             complement(5817..6827)
                     /locus_tag="P5558_08280"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRY24345.1"
                     /translation="MDIRRGTLALIGNTPLVRLNHLSDETGCEILGKCEFMNPGGSIK
                     DRAALAIVEDAEARGLLRPGGVIVEGTAGNTGIGLAVVGNAKGYRTVIVIPETQSQEK
                     KDMLLAQGVELIQVPAVPYRDPNNYVKYSGRLAAELAEKEPNGAIWANQFDNTANRTG
                     HYRQLGPEIWRQTDGEVDAFTCAAGTGGTIAGVGQYLKEQNPKVRIQLADPMGAALYH
                     FYKHGELKAEGSSITEGIGQGRITANLDGAPIDDAYQIPDEEALPVVFDLIRREGLVL
                     GGSSGINVASAVRLARELGPDHTIVTVLCDSGSRYTSRLFNPTFLAGKHLPIPPWMKP
                     GA"
     gene            6765..8381
                     /locus_tag="P5558_08285"
     CDS             6765..8381
                     /locus_tag="P5558_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY24346.1"
                     /translation="MVEAHQWCVTDEGEGAAANVHGSLGKQEVSLTSHVETGGGHDKA
                     GVDAIAALLPGPVERIETHAAIILLTPDRAYKLKKPVAFSFLDFTTLEARQAALEAEL
                     RLNRRTAPEIYERVLPVVASASGLALDEPGEPVEWLLEMRRFPEAGRLDHVAERGELD
                     AALVEALARTIAAFHERLEPLVEAGGAAAMREVADGNAEDLRRSVPAVFASEPVEALV
                     ARTAAELDRQAGLLDARRAAGLVRHCHGDLHLANIVLLDGHPVLFDCIEFNDAFARVD
                     LLYDLAFLVMDLVDRGYRPAAQGLLQAWNERCEDDAGMALLPLFLSIRAAVRAKVEGF
                     AGHGAPACGYLDLASRALEPVPPRLIAIGGRSGTGKSTVARLLAPGMGAMPGAFVLRS
                     DVIRKRLFGAEPEATLPAEAYAEAVSTEVFERLAARAEVLLEAGRTVIADGVFGQGWQ
                     RQRIAAAAAARGAPFAGFWLEAAQAILEDRVAGRTGDASDADVEVVRAQAAMDTSRID
                     WRRIDAAGGAAGVAATIRQALGQPLPAGDR"
     gene            8520..9419
                     /locus_tag="P5558_08290"
     CDS             8520..9419
                     /locus_tag="P5558_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24347.1"
                     /translation="MTTLYLNRHLPDDERRARVFDGDFFLYTEVRGSRALCALAQEMA
                     EEAFAGLDPERAQFDMGVEEFIQRVGPLKSGFTNSARTKELCQIYLTELGCDPLRTYF
                     DLPRLRVVPSDGYLTSGVSYAYKAHRDTWYAHPRQLVNFWIPAWPVVGDNTMSMFVRY
                     FDEQVANGSAGFDYDDWVANSRFAAAGQVKGEARPHPLPRQSIDTRGEIRIAGGAGDA
                     MLFSTCHLHATAPNCSGVTRFSVDLRTVHLDDLMDGRGPALDDSEATGTTLGDFLRVA
                     DLEPLDVGVLPEGIRRQRLAVPA"
     gene            complement(9498..10163)
                     /locus_tag="P5558_08295"
     CDS             complement(9498..10163)
                     /locus_tag="P5558_08295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY24348.1"
                     /translation="MSPRNLALDGALQTYLDELGGREHPELARLRAATDALADGGMRS
                     STVQVQLLALLIELIDARRVLEIGCFTGYGSLGMALALPPEGRLTTLDVNDDWPALGQ
                     RHWQEAGVARRIDFRQGLAMDSIDDLIAEGASGSFDLVYVDAEKKGYPQYYEAALGLL
                     RAGGLVALDNMLWKGAVADPDDRSRQTLTLRALTREIHGDERVTPVLLPIGDGLLLAR
                     KRG"
     gene            complement(10181..10654)
                     /locus_tag="P5558_08300"
     CDS             complement(10181..10654)
                     /locus_tag="P5558_08300"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRY24349.1"
                     /translation="MSELKEGDMMPAFELPTDGGGTVSSESLKGKPYVLYLYPRDDTP
                     GCTKEAQGFTEHKAAFAALGVPVVGLSKDDVASHDKFKTKYDLDLVLASDESGKLVEG
                     LGAWVEKSNYGKTYMGIDRSTFLVDGDGRIVRAWRKVKVPGHVEEVLDAARALGN"
     gene            complement(10651..13662)
                     /locus_tag="P5558_08305"
     CDS             complement(10651..13662)
                     /locus_tag="P5558_08305"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HRY24350.1"
                     /translation="MSLPLPTVASPQPPQSSDGRDEPWLARLRAAAVRHADPSVNDRL
                     ETCLGQRENRDLLAAVFGFSPFLADCLTTDPAIVADFAERGAAAIVEEELARIDALPF
                     DRNRLLPGLRQARRRIAAAVGLADLAGRFDLDQVTRSLSRLADHAAEAVLATFLAESA
                     KRGEIELADTEQPTRDCGIFIVGMGKLGAHELNYSSDIDLIVLFDPDRIDYRGRETAM
                     ALAVRAARAIVYGLEHKSRDGYVFRTDLRLRPHPPGHPLALSVDAAEIYYERHGQNWE
                     RAAFIKARIIAGDRPAGETFLRRLAPFVWRRHLDYAAIRDIHSIKRQINAHRGFGTIG
                     VRGHDLKVGRGGIREIEFFVQTQQLILGGRHPELRDPRTLTTLEKLVEGRWVDGAAGD
                     DLDQAYRFLRALEHRLQMVADKQTQTLPTRDNELEQFARFAGFADTDTLSAAIMAVLQ
                     SVERHYAQLFEAEPDLGAGRQLVFTGTEDDPGTLETLREMGFKDPAAAAGRIRQWHHG
                     HIRATRSTRAREILTELTPGILNALQRQADVDEAFRLFDAFLAGLPAGVQFFSLVRAN
                     PNLLTLIGDLMGAAPRLAQVLAGNVALFDAMLAPDFFEPLPDEAALEAEFAGLRRDAR
                     HLEGLLDLCRRWAQGRQFQAGLHVLLGLADAATASRALTSIAELVIRTLLPEAESWLE
                     EQHGRIAGGSFCVLGLGKLGSRELSLGSDLDLVFLFEGPDDAVSDGAKPLPATTYYAR
                     LGQRLVSAISARTAEGRLFEIDTRLRPSGNVGPIACSLGNFRRYQETTAQVWERQSLT
                     RARAVAGDPGLVADATAAIDEALARPAEPELLAREVRAMRLRIFKEHGSDSPWNLKHC
                     RGGLVDLEFAAQYLVLRHGPDHPSLRRTDTGGILEAAIEAGLIEAAVGRQLLQALALL
                     QALQAVLRLSLSDRFEPRQAPMGLREALVRAANRQLGLGVPVAHFAAVEELLVECETA
                     ARQFFDALCPPEEDGEPTITNPSEPTRGKTP"
     gene            complement(13770..15011)
                     /gene="tyrS"
                     /locus_tag="P5558_08310"
     CDS             complement(13770..15011)
                     /gene="tyrS"
                     /locus_tag="P5558_08310"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRY24351.1"
                     /translation="MSRYRSELLRTLEERGYIHQCTDAEALDELAVDGRVVAYVGYDC
                     TADSLHIGHLVSIMMLRVLQRAGHTPIALVGGGTTKVGDPSFRDESRPLLDDAAIEAN
                     KAGIERSLGAYLDFGGSAIMVDNAEWLDELRYIPFLRDFGTHFTINRMLTFDSVKLRL
                     EREQPLTLLEFNYMVMQAYDFLELGRRHGCQLQMGGSDQWGNIVNGVELGRRIDGRQL
                     FGLTTPLITTASGAKMGKTAAGAVWLNDDRLVPFDYWQYWRNTEDADVGRFLRLFTEL
                     PLDEIARLERLAGAEINEAKKILAFEATALRHGAEAAEAAAAAARAQFEEGGPATGLP
                     SVTVDAARLAAGMAIIELFQLAGLATSNAEARRLVRGGGARLNDERVDDELLVVDGSA
                     LTDGRLKLSAGRKRHVLVEAG"
     gene            complement(15085..15753)
                     /locus_tag="P5558_08315"
     CDS             complement(15085..15753)
                     /locus_tag="P5558_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY24352.1"
                     /translation="MPEVIFNGSDGRLEGRYVHGEGPTPPLAIILHPHPQYGGTMNNR
                     LVHLMFHMFARRGFSTLRFNFRGVGRSQGVFDHGQGELRDAAAALDWMQTHNPNSPIA
                     WCGGFSFGAWIAMQLLMRRPEIQGFMCAGLPANMYDFSFLAPCPSSGLIIHGERDMVT
                     PPEAVEKLVNKLSQQRGITIDFHQIDSADHVFTNDRDEFEAICEAYLDKRLKPTAEPM
                     ALAR"
     gene            16043..16759
                     /gene="cysE"
                     /locus_tag="P5558_08320"
     CDS             16043..16759
                     /gene="cysE"
                     /locus_tag="P5558_08320"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRY24353.1"
                     /translation="MFKRIRDDIEAVFARDPAARSRAEVLLCYPGLHALLLHRLAHRL
                     WTSGWLLLARWISQLGRFLTGIEIHPGARIGERFFIDHGMGVVIGETAEIGDDVTLYQ
                     GVTLGGVSGDPGKRHPTLEDGVVVGAGAAVLGPFTVGRGARIGSNAVVLQAVEPHSTV
                     VGVPGHAVARRLATAGKSCFPAYGNEPGATADPVLVAIDQLCSRVEQLENQLAAVHGQ
                     GADQQADAETPRVLVANLRR"
     gene            16756..17850
                     /locus_tag="P5558_08325"
     CDS             16756..17850
                     /locus_tag="P5558_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY24354.1"
                     /translation="MTARAYLDHAASSPLRPAARAAIAQALDLAGNASSVHEEGRAAR
                     AAVESARRSLATAIGGAAGRMVFTSGATEANAAALAATGRVLASAVEHPSVLAQPGVE
                     TVPVDRSGRLDLAALATALESGNVACVALMLANNETGVLQPVAEAAALAHRAGARLHV
                     DAVQAFGRLPFDVAALGADSVALSAHKLGGPKGAGALWLRDPDAFQPLLRGGGQEHRR
                     RAGTENVAGIVGFAAAAGAIEPEEAGRLAALRRALETEARLSTGDAAVIAAAVPRLPH
                     ITALALPGMAAATQVMALDLAGVAVSAGAACSSGKVATSHVLAAMGVEPALAESTIRV
                     SLGWSTTAGDIDRFLDAWGSLAGRRGRVAL"
     gene            17934..18266
                     /locus_tag="P5558_08330"
     CDS             17934..18266
                     /locus_tag="P5558_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRY24355.1"
                     /translation="MPDALVDLTPAAVARVRDLLSSEETAAGLRLGVKSTGCSGMSYK
                     LDFAREIGADDTVIEKDGVKVVVDPGAVMFVLGTEIDWVEDKLGAMFVFRNPNEKARC
                     GCGESFSV"
     gene            18286..18603
                     /locus_tag="P5558_08335"
     CDS             18286..18603
                     /locus_tag="P5558_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY24356.1"
                     /translation="MTFVLPDGREISADAMAGASLLEVARDLDIAIEGACGGAMACAT
                     CHVIVEPDQFARLPAPSEEEEEMLDLAESLAATSRLGCQIRVETRVDGLVVRVPRTSL
                     LDG"
     gene            18596..19744
                     /gene="mnmA"
                     /locus_tag="P5558_08340"
     CDS             18596..19744
                     /gene="mnmA"
                     /locus_tag="P5558_08340"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRY24357.1"
                     /translation="MAEIDLAKLALSPGERVVVAMSGGVDSAVTAALLHEAGCDVVGI
                     TLQLYDHGVATGRKGACCAGQDIVDARNVADAIGIPHYVLDFEARFKAAVIDDFAQSY
                     AAGRTPIPCVRCNQRIKFRDLLEVARDLGAVALATGHYARRVGGRAGAGLWRARDAQK
                     DQSYFLFATTQDQLDFVRFPLGGLDKVATRIAAARLALPVARKPESQDICFVPTGHYS
                     NIVGRLAPRAMRKGAILHVDGRQLGEHAGIGRFTVGQRRGLKVAEGEPLYVVGLDAEE
                     AVVHVGPRAAGLTGGLTITDCNWLGRPAVGERLAIKHRYNEPAVTGRLAEPTGGDTAR
                     LAFDEPQAGVAPGQAAVLYAGERLLGGGWIERTSKPEAEEAPAVALGA"
     gene            19781..19857
                     /locus_tag="P5558_08345"
     tRNA            19781..19857
                     /locus_tag="P5558_08345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19815..19817,aa:Met,seq:cat)
     gene            complement(19951..20151)
                     /gene="rpmF"
                     /locus_tag="P5558_08350"
     CDS             complement(19951..20151)
                     /gene="rpmF"
                     /locus_tag="P5558_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRY24358.1"
                     /translation="MAVPRKKTSPQKRNQRRAHDALTLPSVIEDKDSGEFRRPHHIDL
                     KTGMYRGRQVIVQKAEVADDDD"
BASE COUNT         2932 a         7064 c         7425 g         3086 t
ORIGIN      
        1 gtaagccagc accgcggcac ccagatgagg cacgtcgccg taggtgtagc cggcggcgac
       61 ggtgcaggtc aggactcctg gccggccctc cgccgcgtgc gccgccgcga gaatccgcgc
      121 catgggtggt gcggtcgtcg cctgcttcac cgtcgccggc aaaagcggca gcttgcggaa
      181 agccttggcc ggtcgctcca tcccgtcgag cagccggccc aggatcgagg ccgcttcctc
      241 gccccgctcg aagaaatcgc tgtgcgggaa cgtgtcgtag ccgacgagga cgtcggccgc
      301 gtccaccagc ccttgcgaga tgttcgcgtg atagtcgagc gtggcaacga cgggcgcgtt
      361 gggtgcgaga aacccccgca caaaacgcac gaccccggca tcggcatcct cccggcccgc
      421 cacatccatg gccccgtgca ggacgacgag cgcgccgtcg aagggggcga gcggtcgcgc
      481 atggtgctcg atgacgccga gaagtttctc gaacgccccg gcctcgacga ggccggcggg
      541 catggcgaag gtgaagagtg tggggacgag ctcgagcccg gcggcggcag cgcccctgat
      601 catcccgccg atctcggtcc cgctcccggc atagcgctcg atgatcgccg aaccttcggc
      661 atactgatag ctgcgaaagt cggcgagggt cgtccgtagg ttcgagaagc cgttggtctc
      721 gtgctggatg ccggccagga gaatgcgtcg gtcagccatc attcacctta ttttgccctg
      781 ctcgccgcag gatcgtgttc ccgcaaggcc tggggctcag ggggccgaac gatgaccggc
      841 ctgtcaagcg gatcgggccg cgtctccggg gccgaggtgt cgttccggcc gcccctgatt
      901 gcggggtcgg tgatcgtcac gtatgtcccg atggtcgtcc tctggctgcc gcgggtgcac
      961 ggcttctgag gatgcaggga caggatttcc ggagaaggag cgaatgatgg gcaaaattcg
     1021 gcctaaccgc gccaaggaga agctcgcggc cggggggatc gtcgtttcgc tcggcgggct
     1081 cgcctcgagc gacctcatcg accagatcgg cccgatgggg ctcgacgcgg tctggctcga
     1141 ggccgagcac ggtccggtcg actacggcca tatcggcgac ctcacccggg cctgcgacct
     1201 ctggggtatg acctcgatcg tccgcgtcaa ttcgctggac tacggcacga tctaccgtgt
     1261 cctcgatctc ggcgcccagg ccatctgcgt gccgcatgtc gacaccgcca acgatgccaa
     1321 gggcttcgtc gaggccgcca agttcgcgcc gatcgggcgg cgcgggctct tcacgggccg
     1381 gcagggcttc ggcgtgcccg actacctcga ggtcgccaac gaccagacca tgctgatcgc
     1441 cctcatcgag gatgcgaagg cggtcgagaa cctcgatgcc atcctcgccg tcgaccatat
     1501 cgacgtcttc ttcgtggccc cctcggacct cgccgcctcc atgggccaca tcggcaagat
     1561 ccagcacccg gaggtgcagg gcaccatcga ccggaccctg cagcgcatcg ccgccgccgg
     1621 tcgcaccccc ggcacgctgc tgctcggcca ccagaatgtc gcgcgttaca ccgctctcgg
     1681 cgtgcgctgc ttcatgaccc cggtgctgcc cctgatcgcc gaaggtgtcg gcagtgtgct
     1741 cgaagccgcg gcggcgtccg gccggcgctg aggcggggcg tcaggcgccc ggtcggtgcg
     1801 cctcgaggtc ggcgagcgcc gccttgcgca gcagcatcga gtcgacggca cccgcgacca
     1861 tggtgtagcc ggcggcgaag agctcgggca cggtgcgcat cgccgaagga acggtgccga
     1921 gcggcttgcc ggaggggcgc aggttcctct cgcagcgctc gaccagctcc ctgacctccg
     1981 ggtggtcgag ctggccgagg cggccgatcg agcccgccat gtcgttgatg ccgatgaacg
     2041 gcatgtcgat gccgtcgacg gcggcgatcg ccgccgcctc gtcgacggcc ctggcactct
     2101 cgagctggcc caggatcatg agccggtcgt tccattcggc gagataattg gcgtccttgc
     2161 cccagcgggt gcagcgatgg gacggcgccg cgtagccgcg cgtgccgtgc ggcgggtagc
     2221 ggcaggcggc caccgcggcc ttggccgcct ccgcaccctc gagcagcgga atcatcaccg
     2281 aggtggcgcc tagatcgagg atgcgcttga gatagacctg atcgttccag ggcacgcgga
     2341 cgaccagggg gcagccggcg gcctcggccg cacgcatgac gtcggcggcc gtctccaggc
     2401 tgccggggcc gtgctcctgg tcgaggatca ggaaatcgta gccggcatgc gccatgagct
     2461 cggcgacggc agcatcggcg aggccgagcc aggcgccata aacgggctcg ccggccagca
     2521 cgcggcgttt cagagcattc gtggtcatct gcgtcccctt ccgagcggcc ggtgcgccgc
     2581 cttcggcccg ggtctatcgg tcagcggccc cggtcggcaa gggtgctcgt catctacggg
     2641 ccgagacgca cggcgagggt cgggcccggg ccgtgcacgc tgtcctcggc ctggaccagg
     2701 acctcgtcgg tgccgggcgg gatcgccacg ccgccgagcg agcgggtgaa gggctgctcg
     2761 tcctcgtggg ggtgcaggag cacgcgctcg ccgagcacct gcccgtcggg cgtcagcacc
     2821 acccacttgt cggcatagtg atcccagccc tcgtcgccgt gccggacggt gacgtcgaac
     2881 cgccaggtgc cggcggcctc cgggcgcgcc tcgaccccct cgaccaccgg ctcgccggcc
     2941 ttcgctgcat gggccgccag cagcagcgcc agtccgaagc caaggctcct catcactgat
     3001 tcccccgaca gtccaggttc tcgatctcga tcggtggcaa aggctccgcc gggacgatgc
     3061 cgaaaatccg gcaataaaag gcatattcgg cgagcagcac cgtcctctgc acgtccgccg
     3121 agcggaagcc gtggccctcg ccctcgaact cgaggcaggc gacgggcagc ttcttggcgc
     3181 gcagcgcgtc gcgcatggcc caggcctggt cgggcgtcac caccttgtcc tcgaggccct
     3241 ggaagaggat gagcgggcag ctcagccggt cggtgtggtg aatgggcgag cgctcgcggt
     3301 acacctcggc gctttcgggc caggggccga tcagccggtc gagatagcgc gcctcgaact
     3361 tgtgggtggt ccgcatcaac gcttcgagat cgccgatgcc gtaatgcgag gcgccggcct
     3421 tgaaggtgtc gtggaaggtg agggcggcga gcgtggtgaa gccgccggcg ctgccgccgc
     3481 tgatgaccag gcgatcgggg tcggccagcc cctcggcgac gaggtgctcg gcggcggcga
     3541 tgcagtcctc gacatcggcg atgccccact ggccgcgcag gctgtcgcga taggcgcgcc
     3601 cgtagccggt gctgccggca tagtcgacgt cgacgatggc gaagccgcgg ctcgtccaga
     3661 actggtagcc gaggcggagc gccggcgagg tggcactggt cggaccgcca tggctgcgga
     3721 cgatcaaggg cggcttctcg ccctcgggtg cgcgaaagtc cgggttggtc ggcgggtagt
     3781 ggaaagcgtg cgcggtgcgg ccgttgcggc tcggaaagct gatcggtcga ggtgccgaga
     3841 tccaggccgg atcgagctcg agcggcccgg cggcacagag aatctccagc ttgcggctct
     3901 cgaggtccag ggccgcgatc tcggccgtgc ggtccagcgc cgagccgcga aagacggccc
     3961 ggccgccggc ggcgtgaatc tcggtgaagt cggcgaaggg cagatccatg gtcgcgagcc
     4021 ggccgtcctc gaggtggagg atgccgaggc ggccgagacc gtcctcgccc caggcgacga
     4081 ggcagcgcct cgcgtcgatg ggcgtccacc agcggttgcc gacctgccag agggggccgc
     4141 cgaaatcgct gtcgcgctcc aagaggggtg cctcgccgcc ctcctgccag aggttccaga
     4201 agcccgtgcg gtcgctgagg tagaggagcc gcccgtcggg caggaactcc ggctgcagca
     4261 ccgcctcacc gccacccgcc ccggcgacac aactcacgtc ggtcggcgtt ccgtccgaag
     4321 ccagccgtgc tgtcatcagc cgcgtcgcgt cccagggcat gtccggatgg tcccagacca
     4381 gccaggcgag ccggcggcca tcggaagaca ggcgcggaca ggcgaagaaa tcgtggctct
     4441 cggccagaat caccggctcg ccgccggcga gcggcagggc gaccagaagg ttggccggct
     4501 cgccgccggt gcggtggtcc tcggccacgg ccacgacgcg ctggcgctgc cagtcgatca
     4561 ggggctcggc gaaccgcaat gacccgccgg tgtcggggct gatcggccgg atcgagccgt
     4621 cggcgtcccg gcgatacaga cgctggtcgc ggtcattcac caggacgacg aggccatccc
     4681 gcgcggcgac acacgaccca ccgtattcgt gcagccgcgt gcgaacgttg agcggcgccg
     4741 gggtcagctc ggtggcctgc ctgccgccgc ccggccggtc cagggccatg agggaaaccc
     4801 tgccgccttc ggccggccgc ccctcgagcc agagaacgcg ctcgccatcg agcacgatct
     4861 ggcccagccg gttctgggca ccggtggctt tcgccgcact gagcggcgag ggccagccgc
     4921 cgaaaggtcg ctcctgcacc ccgaatgccc gccgggtcag cgggccaccg gcgtgtcggg
     4981 gcggctcccc cattcggtcc atgagccgtc gtagagcgcc acgtcgggcc ggccgagccg
     5041 ggcgagcgcc aggctggcga cggcggccga gaccccggag ccgcagctcg tcgtgagctt
     5101 ctggtcgaga tcgaccccgg cggctgcaaa gagggcggtg agcgcctgcg cgtccttgag
     5161 cgtgccctcg gcggtgacga ggtcgctggc cggcaggttg cgcgagcccg ggatgtggcc
     5221 ggcccggagc ccctcgcgcg gctcggcgac gctgcccgtg aaccgggcgg ccgagcgggt
     5281 gtcgaccacc tgctcgcgcc ggctggtcag gttggcgcgc atctgctcga gatcgcgcac
     5341 gaggaagttg ttcagccgcg gggtgaaatg gcgctcggtc gggcgaacgg gcacatcgtc
     5401 gacgggacgg ccctcggcgc gccatttggc gagcccgcca tcgagcacgg cgatgtcctc
     5461 gtggcccatc acccggaaca tccaccagac gcgggcggag gcgtagaagc ggttgttgtc
     5521 gtagacgacg atgcgcacgc cgtcgccgag cccgagcttg cgcacccggg ccgagaactt
     5581 cgccgggctc ggcagcatgt gcggcaggtc ggtgtcgctc tccgagatct catcgatgtc
     5641 gaagaagacg gcgccgggca gatgcgccgc cgtgtgctcc gccttcgggt cgcgaccttc
     5701 ggcgggcatg aaccaggtgc agtcgacgag gcggatgtcg ggcgcctcca ggcgctcggc
     5761 gagccagtcg gtcgagacga gagggtcggt caaggtgctg ctccctggcg ggccgctcac
     5821 gcgcccggct tcatccaggg cgggatcggc agatgcttcc cggcaaggaa ggtagggttg
     5881 aagagcctcg atgtgtagcg gctgccggag tcgcagagca cggtgacgat ggtgtggtcc
     5941 gggccgagct cgcgggcgag gcggacggcc gaggcgacgt tgatgccgga cgagccgccg
     6001 agaaccaggc cctcgcgccg gatcaggtcg aagaccaccg gcagcgcctc ctcgtcgggg
     6061 atctggtacg cgtcgtcgat cggcgcgcca tcgaggttgg cggtgatccg gccctggccg
     6121 atgccctcgg tgatcgagct gccctcggcc ttgagctcgc catgcttgta gaaatggtag
     6181 agggcggcac ccatcgggtc ggcgagctgg atgcggacct tggggttctg ctccttcagg
     6241 tattggccga cgccggcgat cgtcccgccc gtcccggcag cgcaggtgaa agcatccacc
     6301 tcgccatccg tctggcgcca gatctcgggg ccgagctggc ggtagtggcc cgtccggttg
     6361 gcggtattgt cgaactggtt cgcccagatg gcgccgttgg gctccttctc ggcgagctcg
     6421 gcggcgaggc ggccggaata tttcacgtag ttgttgggat cccggtacgg cacggccggc
     6481 acctggatga gctcgacacc ctgggccagg agcatgtcct tcttctcctg gctctgcgtc
     6541 tcggggatga cgatcaccgt ccggtagccc ttggcgttgc cgaccacggc gaggccgatg
     6601 ccggtgttgc ccgccgtgcc ctcgacgatc acgccgcccg gccgcagcag gcccctggcc
     6661 tcggcatcct cgacgatggc gagcgccgcc cggtccttga tgctgccgcc cgggttcatg
     6721 aactcgcact tgccgagaat ctcgcagccg gtctcgtccg acaggtggtt gaggcgcacc
     6781 agtggtgtgt taccgatgag ggcgagggtg ccgcggcgaa tgtccatgga tctctcggaa
     6841 aacaggaggt ttcactgacc agtcatgtag agacgggcgg ggggcacgac aaggcggggg
     6901 tcgacgcgat cgcggccctg ctgcccgggc cggtcgagcg gatcgagacg catgccgcca
     6961 tcattctgct gaccccggac cgcgcctaca agctgaagaa gcccgtggcc ttcagctttc
     7021 tcgacttcac cacgctcgaa gcccgccagg cggcgctcga ggccgagctg cggctgaacc
     7081 ggcgcacggc acccgaaatc tatgagcgcg tcctgccggt ggtcgcgtcc gcgtccggcc
     7141 tcgctctgga cgagcctggc gagcccgtgg aatggctcct ggagatgcgc cgctttccgg
     7201 aggccggccg gctcgaccat gtggccgaac gtggcgagct ggacgcggcc ctggtcgagg
     7261 cgctggcccg gaccattgcc gcgtttcacg agcgcctgga acccctggtc gaggcgggcg
     7321 gcgccgctgc catgcgcgag gtggcggacg gcaatgccga ggatctgagg cgctcggtgc
     7381 cggcggtttt cgccagcgag ccggtcgagg cgctcgtggc acgaacggcc gccgagctcg
     7441 accggcaggc cgggctcctc gacgcgcgcc gcgccgccgg cctcgtccgc cactgccacg
     7501 gcgacctgca cctcgccaac atcgtgctgc tcgacggcca cccggtgctc ttcgactgca
     7561 tcgagttcaa cgatgccttc gcccgggtcg acctgctcta cgacctcgcc ttcctcgtca
     7621 tggacctcgt cgatcgcggc taccggcccg cggcgcaggg cctgctgcag gcctggaacg
     7681 agcggtgcga ggacgatgcc ggcatggcgc tcctgccgct cttcctctcc atccgggcgg
     7741 ccgtgcgcgc caaggtcgag ggattcgccg gtcatggcgc accggcctgc ggctatctcg
     7801 acctcgccag ccgcgcgctc gagccggtgc caccccggct gatcgccatc ggcggccgtt
     7861 ccggcaccgg caagtcgacc gtggcccggc tgctggcgcc ggggatgggg gccatgccgg
     7921 gtgcgttcgt gctgcgctcg gacgtgatcc gcaaacggct cttcggcgcc gagccggagg
     7981 cgacgctgcc ggctgaggcc tatgccgagg ccgtctcgac cgaggtcttc gagcgcctcg
     8041 cggcccgggc ggaggtgctg ctcgaggcgg ggcggacggt gatcgcggac ggggtcttcg
     8101 gccagggctg gcagcggcag cggatcgccg ccgccgcggc ggcccggggc gcacccttcg
     8161 ccgggttctg gctcgaggcc gctcaggcca tcctggagga ccgggtggca gggcggaccg
     8221 gcgatgcgtc ggatgccgat gtcgaggtcg tgcgtgccca ggcggcgatg gacacgagcc
     8281 gcatcgactg gcggcggatc gacgccgccg gcggcgcggc aggcgtcgcg gcgaccatcc
     8341 ggcaagcgct cggccagccc ttgccggcgg gggatcgata gggcggcagc cccgccgtat
     8401 ggcgcccctc gcaaaagggg gatgacaggc cctgcggttg tcctataatt ttcctagaaa
     8461 ttgcctaggt cagcctcggt cggtccagac cgggcgccgc ggcacgcggg gtgacgccga
     8521 tgaccacgct ctacctcaat cgccatctgc cggacgacga acgccgcgcc cgggtcttcg
     8581 acggcgactt cttcctctac acggaggtgc ggggctcgcg ggccctctgc gcgctcgcgc
     8641 aggagatggc ggaggaagcc ttcgccgggc tcgatccgga gcgcgcccag ttcgacatgg
     8701 gtgtcgagga gttcatccag cgggtgggcc cgctcaagtc cggcttcacc aacagcgcgc
     8761 gaaccaagga gctctgccag atctatctga ccgagctcgg ctgcgatccg ctgcgcacct
     8821 atttcgacct gccgcggctc agggtcgtgc cgtccgacgg ctacctgacc tcgggcgtca
     8881 gctatgccta caaggcgcat cgggacacct ggtacgcgca cccgcgccag ctcgtgaatt
     8941 tctggatccc ggcctggccg gtcgtcggcg acaacacgat gtcgatgttc gtgcgctatt
     9001 tcgacgagca ggtggcgaac ggctccgccg ggttcgacta cgacgactgg gtcgccaaca
     9061 gccggttcgc cgcggcgggc caggtcaagg gcgaggcgcg gccgcacccc ctgccgcgcc
     9121 agtcgatcga cacgcggggc gagatccgta tcgccggcgg cgccggcgac gccatgctgt
     9181 tctcgacctg ccacctgcat gcgacggccc cgaactgctc gggcgtcacg cggttcagcg
     9241 tcgacctccg gacggtgcac ctcgacgacc tcatggacgg tcgcggcccg gcgctcgacg
     9301 acagcgaggc caccggcacg acgctcggcg atttcctgcg ggtcgccgac ctcgagccgc
     9361 tcgatgtcgg cgtgctgccg gaggggattc gacggcagcg cctggccgtg ccggcctgag
     9421 gccgggcctg ccgacctcca aacgaggacg ggcgagacca ggccgaaagg gtccagcccg
     9481 ctcagcgcct ccccggatca gccgcgcttg cgcgccagca gcaacccgtc gccgatcggc
     9541 agcagcaccg gcgtcacccg ctcatcgccg tggatctcgc gggtcagcgc gcgcagcgtc
     9601 agggtctgcc gcgaccggtc gtcgggatcg gccacagcac ccttccagag catgttgtcg
     9661 agggcgacga ggccgcccgc ccgcaagagg ccgagcgccg cctcgtagta ttgcgggtag
     9721 cccttcttct cggcatcgac atagacgagg tcgaagctgc ccgacgcacc ttcggcgatc
     9781 aggtcgtcga tgctgtccat ggcgaggccc tggcgaaagt cgatgcgtcg ggcgacgccg
     9841 gcctcctgcc aatgccgctg gccgagcgcc ggccagtcgt cgttgacgtc gagcgtggtg
     9901 agccggcctt ccggcggcaa agcgagggcc atgccgaggc tgccgtagcc cgtgaagcag
     9961 ccgatctcga ggacgcggcg cgcgtcgatg agctcgatca gcagggccag gagctggacc
    10021 tggacggtcg agctgcgcat gccgccatcg gccagcgcat cggtcgccgc gcgcaggcgg
    10081 gccagctccg ggtgctcacg cccgccgagc tcgtcgagat aggtctgcag cgcgccatcc
    10141 agcgccagat tgcgagggct caagatccgg tctccccggc tcagttgccg agcgcgcgcg
    10201 cggcgtcgag cacttcctcg acatggcccg gcaccttgac cttgcgccag gcccggacga
    10261 tccggccgtc gccgtcgacg aggaaggtgg agcggtcgat gcccatgtag gtcttgccgt
    10321 aattgctctt ttcgacccag gcgccgagcc cctcgaccag cttgccgctc tcgtcggagg
    10381 cgagcaccag atcgaggtcg tacttcgtct tgaacttgtc gtggctcgcg acgtcgtcct
    10441 tggagaggcc gacgaccggg acgcccagcg ccgcgaaggc ggccttgtgc tcggtgaagc
    10501 cctgcgcctc cttggtgcag cccggcgtgt cgtccctggg atagagatag agcacgtagg
    10561 gcttgccctt caggctctcg ctcgagaccg tgccgccccc gtcggtcggc agctcgaacg
    10621 ccggcatcat gtcgccttcc ttgagctcgc tcatggcgtc tttcccctcg ttggttctga
    10681 tgggtttgtg atggtgggct ccccgtcctc ttcgggcggg cagagcgcgt cgaagaactg
    10741 gcgtgccgcc gtctcgcatt cgacgagcag ctcctcgacc gccgcgaaat gagccacggg
    10801 cacgccgagg ccgagctggc ggttggcggc ccgcaccagg gcctcgcgca ggcccatggg
    10861 cgcctgccgc ggctcgaacc tgtcgctcag ggagaggcgg aggacggcct gcagcgcctg
    10921 caggagggcc agcgcctgca ggagctggcg gccgaccgcc gcctcgatca ggccggcctc
    10981 gatggctgcc tccaggatgc cgcccgtgtc ggtgcgccgc aggctcggat ggtcggggcc
    11041 gtgacgcagc acgagatact gtgccgcgaa ctcgagatcg acgaggccgc cccggcaatg
    11101 cttgaggttc caggggctgt cgctgccgtg ctccttgaag atccgcagcc gcatggcgcg
    11161 gacctcgcgt gctaggagct ccggctcggc cggtcgcgcc agggcctcgt cgatggccgc
    11221 ggtcgcatcg gcgacgaggc cgggatcgcc ggcgacggcc cgggcacggg tcagcgactg
    11281 gcgctcccag acctgcgccg tggtctcctg gtagcggcgg aaattgccga ggctgcaggc
    11341 gatcggtccg acattgccgg agggccgcag acgcgtgtcg atctcgaaga gccgaccctc
    11401 ggccgtccgg gcgctgatgg cgctgacgag gcgctggccg agccgggcat agtaggtcgt
    11461 ggcgggcaag ggcttggcgc cgtccgacac cgcgtcgtcg gggccctcga agaggaagac
    11521 gaggtcgagg tcggagccga ggctgagctc gcgcgagccg agcttgccga ggccgagcac
    11581 gcagaagctg ccgcccgcta tccggccgtg ctgctcctcc agccagctct cggcctccgg
    11641 caggagcgtg cggatgacga gctcggcgat gctggtgagc gcccggctgg cggtggccgc
    11701 atcggcgagc ccgagcagca cgtgcagccc cgcctggaac tggcggccct gggcccagcg
    11761 ccggcagaga tcgagcagcc cctcgagatg ccgcgcatcg cgccggaggc cggcgaactc
    11821 ggcctcgagc gccgcctcgt cgggcaaggg ctcgaagaaa tcgggggcca gcatggcgtc
    11881 gaagagcgcc acgttgccgg ccagcacctg cgccagccgg ggggcggctc ccatcaggtc
    11941 gccgatcagg gtgagcaggt tcgggttggc gcggaccagc gagaagaact gcaccccggc
    12001 gggcaggccg gcgagaaagg cgtcgaacag ccggaaggcc tcgtcgacat ccgcctgccg
    12061 ctgcagggcg ttgaggatgc cgggggtcag ctcggtcagg atctcgcgcg cccgggtgct
    12121 gcgcgtggcg cggatgtggc cgtgatgcca ttgcctgatc cggccggcgg cggcggccgg
    12181 gtccttgaag cccatctcgc gcagcgtctc gagcgtgccc ggatcgtcct cggtgccggt
    12241 gaagacgagc tggcgcccgg cgccgagatc cggctcggcc tcgaagagct gggcataatg
    12301 gcgctcgacc gactgcagca cggccatgat cgcggcgctg agggtgtcgg tgtcggcgaa
    12361 gccggcgaag cgggcgaact gctcgagctc gttgtcgcgg gtcggcaggg tctgggtctg
    12421 cttgtcggcg accatctgca ggcggtgctc gagcgcccgc aggaagcggt acgcctggtc
    12481 gagatcatcg ccggcggcac catcgaccca gcgcccttcg accagcttct ccagcgtcgt
    12541 gagggtccgg ggatcgcgca gctcggggtg gcggccgccg aggatgagct gctgggtctg
    12601 gacgaagaac tcgatctcgc gaatgccgcc ccgcccgacc ttgagatcgt ggccgcgcac
    12661 gccgatcgtg ccgaagccgc gatgggcgtt gatctgccgc ttgatcgaat gaatgtcgcg
    12721 gatggccgcg tagtcgagat gccgccgcca gacgaagggc gcgaggcgcc tgaggaaggt
    12781 ctcgcccgcc ggccggtcgc cggcgatgat ccgggccttg atgaaggcgg cgcgctccca
    12841 gttctgcccg tgccgctcgt agtagatctc ggcggcatcg accgagagcg ccaggggatg
    12901 gcccggtggg tgggggcgca ggcgcaggtc ggtgcggaag acgtagccgt cgcggctctt
    12961 gtgctcgagc ccgtagacga tggcgcgggc ggcacgcacg gcgagcgcca tggcggtctc
    13021 ccgcccgcga tagtcgatcc tgtccgggtc gaagaggacg atgaggtcga tgtccgagga
    13081 gtagttgagc tcgtgggcgc cgagcttgcc catgccgacg atgaagatgc cgcagtcgcg
    13141 ggtcggctgc tcggtgtcgg cgagctcgat ctcgccccgc ttcgccgatt cggcgagaaa
    13201 cgtcgcgagc accgcctcgg ccgcatggtc ggcgaggcgg ctgaggcttc gggtcacctg
    13261 gtcgaggtcg aagcggccgg cgagatcggc gaggccgacc gccgccgcga tccggcggcg
    13321 ggcctggcgc aggcccggca ggagccggtt gcggtcgaag ggcagggcgt cgatccgggc
    13381 gagctcctcc tcgacgatcg cggcggcacc acgctcggcg aagtcggcga cgatggccgg
    13441 gtcggtcgtc aggcaatcgg cgaggaacgg gctgaagccg aagacggcgg cgagcagatc
    13501 gcggttctcg cgctgcccga ggcaggtctc gagccggtcg ttcaccgatg gatcggcatg
    13561 gcgcaccgcc gccgcgcgca accgggcgag ccatggctcg tcccgcccgt cgctcgactg
    13621 cggcggctgg ggcgaggcaa cggtcggcaa cggcagggac acggcgatcg accagaataa
    13681 gctgcaggtg tcggctcggg ccacaacgca aggggccgca agttctccct tccgtccggc
    13741 ccggtcaagc gcggatccgc cggccctgat cagcccgctt cgacgaggac gtggcgtttc
    13801 ctgccggcgg agagcttgag ccgaccgtcg gtgagcgcac ttccgtcgac gacgagcagc
    13861 tcgtcgtcga cccgctcgtc gttcagccgg gcaccgccac cccggacgag ccggcgggcc
    13921 tccgcgttcg acgtggcgag gccggcgagc tggaagagct cgatgatcgc catgcccgcc
    13981 gccagccgcg ccgcatcgac cgtgaccgac ggcaggccgg tagcgggccc gccctcctcg
    14041 aactgggcac gggccgccgc ggccgccgcc tcggcggctt cggcgccgtg ccgcagcgcc
    14101 gtcgcctcga aggccaggat cttcttggcc tcgttgatct ccgcccctgc gagccgctcc
    14161 agccgggcga tctcgtcgag cggcagctcg gtgaagagcc gaaggaagcg gccgacatcg
    14221 gcatcctcgg tgttgcgcca gtactgccag tagtcgaagg gcaccagccg gtcgtcgttg
    14281 agccagacgg caccggccgc cgtcttgccc atcttggcac cggaggccgt ggtgatcagc
    14341 ggggtcgtca ggccgaagag ctggcggccg tcgatccgcc ggccgagctc gaccccgttg
    14401 acgatgttgc cccactggtc ggagccgccc atctgcagct ggcagccgtg gcgtcggccg
    14461 agctcgagga aatcataggc ctgcatgacc atgtagttga actcgagcag ggtcagcggc
    14521 tgctcgcgct cgagccgcag cttgacgctg tcgaaggtca gcattcggtt gatggtgaaa
    14581 tgggtgccga agtccctcag gaacgggatg tagcgcagct cgtcgagcca ttcggcattg
    14641 tcgaccatga tcgccgagcc gccgaagtcg agataggcgc cgagcgagcg ctcgatcccg
    14701 gccttgttgg cctcgatcgc ggcgtcgtcg agcagcggcc ggctctcgtc gcggaacgac
    14761 gggtcgccga ccttggtggt gccgccgccg acgagggcga tcggcgtgtg cccggcacgc
    14821 tgcagcacgc gcagcatcat gatgctgacc agatggccga tatgcaggct gtcggcggtg
    14881 cagtcgtagc cgacataggc gaccacgcgg ccgtcgaccg cgagctcgtc cagcgcttcg
    14941 gcgtcggtgc attgatggat gtagccgcgc tcctcgagcg tgcgcagcag ttccgagcgg
    15001 tagcggctca tgggtgtccg atcggtggtc cggctggagg ccaccggcgc cccgagcggc
    15061 gccggccggg cttcgataga ggcttcagcg cgccagcgcc atgggctcgg cggtcggctt
    15121 cagccgcttg tccagatagg cctcgcagat cgcctcgaac tcgtcccggt cgttggtgaa
    15181 gacgtggtcg gcgctgtcga tctggtggaa gtcgatggtg atgccgcgct gctggctgag
    15241 cttgttgacc agcttctcga ccgcctcggg cggcgtcacc atgtcgcgct cgccatggat
    15301 gatcaggccg gaggaagggc agggggcaag gaagctgaaa tcgtacatgt tggccggcag
    15361 gccggcgcac atgaagccct ggatctcggg ccgccgcatg agcagctgca tggcgatcca
    15421 ggccccgaag gagaacccgc cgcaccaggc gatggggctg ttggggttgt gcgtctgcat
    15481 ccagtcgagc gccgccgccg cgtcgcgcag ctcgccctgg ccgtgatcga acacgccctg
    15541 gctgcgcccg acgccacgga agttgaagcg cagcgtcgag aagccgcgcc gcgcgaacat
    15601 gtggaacatc agatggacga gacggttgtt catcgtcccg ccatattgcg gatgcgggtg
    15661 cagaatgatg gccagaggcg gcgtgggtcc ctcgccgtgc acatatcgcc cctcgaggcg
    15721 cccgtccgag ccgttgaaga tgacttccgg catatccctc acactcctgc gccgacaaaa
    15781 ccgacggcga tcgtcccggg tccgtcccgg cggcatggca tctcctggcg gcttcggtga
    15841 accgaccgcg cagaagatca ccccgaatgc agggtgcttc ggccttgatt gaccccgacc
    15901 aacgccaacc tatactccga gcgtgccgac aggcgttctt cgttcgagcc gcgcatcaaa
    15961 gcgtacgtcc cgggaagggg ccgaagccga tgtgcgtggc tggcgttggg cagactgcgg
    16021 cctataccgc atgcgagcgc cgatgttcaa gagaatccgc gacgacatcg aggccgtgtt
    16081 cgcccgcgat ccggcggccc gatcccgggc cgaggtgctg ctgtgctacc cgggcctgca
    16141 cgccctgctc ctgcaccggc tggcccatcg gctctggacg tccggctggc ttctgctcgc
    16201 ccgctggata tcgcagctcg gccggttcct gaccggcatc gagatccatc cgggtgcccg
    16261 gatcggcgag cgcttcttca tcgatcacgg catgggcgtg gtgatcggcg agaccgccga
    16321 gatcggcgac gacgtcacgc tctaccaggg ggtcacgctc ggcggcgtga gcggcgatcc
    16381 gggcaagcgc catccgaccc tggaggacgg cgtggtggtc ggcgcgggtg ccgccgtgct
    16441 cgggccgttc accgtcggcc ggggtgccag gatcggctcg aacgccgtcg tcctgcaggc
    16501 ggtcgagccc cattcgacgg tcgtcggcgt gcccggccat gcggtggcgc gccgcctcgc
    16561 gaccgccggc aagagctgct ttcccgccta cggcaacgag cccggcgcca ccgccgatcc
    16621 ggtgctggtg gccatcgatc agctctgctc acgggtcgag cagctcgaga accagctcgc
    16681 cgcggtgcac ggccagggcg ccgaccagca ggcggacgcc gagacccctc gggtgctggt
    16741 ggccaatctg cgccggtgac ggcgcgcgcc tatctcgacc acgccgcctc gtccccgctc
    16801 cgcccggccg cccgggcggc aatcgcccag gccctcgatc tggcgggcaa cgcctcgtcc
    16861 gtgcatgaag agggccgggc ggcgcgcgcc gccgtcgagt cggcccggcg ctcgctggcg
    16921 acggcgatcg gcggtgccgc gggcaggatg gtgttcacca gtggcgccac ggaagcgaat
    16981 gccgcagcgc tcgcggcgac cggccgggtg ctggcttccg ccgtcgagca cccttccgtg
    17041 ctggcccagc ccggcgtcga gaccgtgccg gtcgaccggt ccggccggct cgacctcgcg
    17101 gcactcgcga ccgcgcttga gtccggtaat gtcgcctgcg tcgcgctcat gctcgccaac
    17161 aacgaaaccg gcgtgctgca gcccgtggcc gaagcggccg cgctggcgca tcgggccggt
    17221 gcccgcctgc atgtcgatgc cgtgcaagcg ttcggccggc ttccgttcga tgtcgctgcc
    17281 ctgggcgccg acagtgtggc gctctccgcc cacaagctgg gcgggccgaa gggcgcaggc
    17341 gccctctggc tgcgcgaccc cgacgcgttc cagccccttc tccggggtgg cggccaggag
    17401 caccgccggc gcgccgggac cgagaatgtc gccggcatcg tcggcttcgc cgcggcagcc
    17461 ggggccatcg agcccgagga ggcgggccgg ctggcggcgc tgcgtcgggc gctcgagacg
    17521 gaagcccgcc ttagcaccgg ggatgccgcg gtcatcgcgg cggcggtgcc gcggctgccc
    17581 cacatcaccg cgctggcgct gcccggcatg gccgccgcga cgcaggtcat ggccctcgac
    17641 ctcgccggcg tggccgtctc ggccggtgcc gcctgctcct ccggcaaggt cgccaccagc
    17701 cacgtgctcg ccgccatggg cgtcgagccg gcgctggctg aatcgaccat tcgcgtgagc
    17761 ctcggctgga gcaccacggc cggcgacatc gaccggttcc tcgacgcctg gggcagcctc
    17821 gccggacgcc ggggcagggt cgcactctag agccgatccg gggcgcccgg ccttgtcccg
    17881 gcggggatcc ggacgacata agggtctgaa gcttgtcgaa cgggattctg cccatgcctg
    17941 acgccttggt cgatctcacg cccgccgcgg tggcgcgggt gcgtgatctc ttgagcagcg
    18001 aggagaccgc tgccggcctc cggctcgggg tgaagtccac gggctgctcg ggcatgagct
    18061 acaagctcga cttcgcccgc gagatcggcg ccgacgacac ggtgatcgag aaggacggtg
    18121 tcaaggtggt ggtcgatccg ggcgccgtga tgttcgtcct cggcaccgag atcgactggg
    18181 tcgaggacaa gctcggcgcc atgttcgtct tccgcaaccc gaacgagaag gcgcgctgcg
    18241 gttgcggaga gagcttctcc gtctgagccc ggggcaaaag tgcgggtgac gttcgtcctg
    18301 ccggatggcc gcgagatcag cgccgacgcc atggccggcg ccagcctgct cgaggtggcg
    18361 cgcgacctcg acatcgccat cgagggggcc tgcggcgggg ccatggcctg cgccacctgt
    18421 cacgtgatcg tcgagccgga ccagttcgcc cgcctgccgg cgccgtccga ggaggaggag
    18481 gagatgctgg acctggccga gagcctggcg gcgacctcca ggctcggctg ccagatcagg
    18541 gtcgagacgc gggtggacgg cctcgtcgtc cgggtgccgc ggaccagcct gctcgatggc
    18601 tgagatcgac ctcgcgaagc tcgccctctc gcccggcgag cgggtggtcg tcgccatgtc
    18661 gggcggggtc gacagcgccg tcaccgcggc cctgctgcat gaagccggtt gcgacgtcgt
    18721 cggcatcacc ctgcagctct acgaccacgg ggtagcgacc ggcaggaagg gcgcctgctg
    18781 cgccggccag gacatcgtgg acgcccgcaa cgtcgctgat gccatcggca tcccgcatta
    18841 cgtcctcgac ttcgaggccc gcttcaaggc ggcggtcatc gacgacttcg cccagagcta
    18901 tgcggcgggt cgcacgccga tcccctgcgt tcgctgcaac cagcggatca agttccgcga
    18961 tttgctcgag gtggcccgcg atctcggtgc cgtggccctc gccaccggcc actacgcgag
    19021 gcgggtcggc ggcagggccg gggccgggct ctggcgcgcc cgggacgcgc agaaggacca
    19081 gagctacttc ctcttcgcca cgacccagga ccagctcgac ttcgtccgct tcccgctcgg
    19141 cgggctggac aaggtcgcga cccgcattgc ggctgcccgc ctcgccctgc cggtggcgcg
    19201 gaagcccgag agccaggaca tctgcttcgt gccgaccggg cactacagca acatcgtcgg
    19261 ccggctggcg ccgcgggcca tgcgcaaggg cgccatcctc cacgtcgacg gccgccagct
    19321 cggcgagcat gccggcatcg gccgcttcac cgtcggccag cggcgcgggc tcaaggtcgc
    19381 cgagggcgag ccgctctatg tggtggggct cgatgcggaa gaggccgtcg tccatgtcgg
    19441 cccgcgcgcg gcggggctga cgggcgggct caccatcacc gactgcaact ggctcggccg
    19501 accggccgtc ggcgagcggc tggccatcaa gcatcgctac aacgagccgg cggtcacggg
    19561 ccgcctcgcg gagccgaccg gcggcgacac cgcgcggctc gccttcgacg agccgcaggc
    19621 cggggtggcg cccggccagg ccgccgtgct ctacgccggc gagcggctgc tcggcggcgg
    19681 gtggatcgag cggacgtcca agcccgaggc cgaggaagcg ccggcggtgg ctttgggcgc
    19741 ttgacgggag gcaccgtcgg tcatatctcc agcgcggctc ggcggagtag ctcagttggt
    19801 tagagcagcg gaatcataat ccgcgtgtcg ggggttcgag tccctcctcc gctaccaccc
    19861 ttcctccgct cggcgggcgt gcccgcaacg aaaaggccgg ccaccagagg tgaccggccc
    19921 ttggtcgttc cgcggctcgc gccggtcgcg tcaatcgtcg tcgtcggcga cctcggcctt
    19981 ctgcacgatc acctgccggc cgcgatacat gccggtcttg agatcgatgt ggtgcggccg
    20041 gcggaactcg ccggaatcct tgtcctcgat caccgagggc agggtgagcg cgtcgtgagc
    20101 ccggcgctgg ttgcgcttct gcgggctggt cttcttgcgt ggcacagcca tggacgtcga
    20161 tcctcaaagg tcgggtgtcg cacgcctccc ggcggcatgg ccgcgggccg aacgggcgat
    20221 ctctcgggaa agctggcgtg cgcttacgcg attggcgcct ccgggtcaac gcggcatccc
    20281 gcgccaaccc tcctccatcg ctccccgcca tggcccgccg gtcgccgaat cgatcatcga
    20341 aaaaaaagtg cttgatcacc ggcgaaggca agcgtagaca tcacctccgc cgcggcgatc
    20401 gaccgtggcg atccctgacg ggcctgttcc tgcaaaccgc gcgagcggcc cgatgttctt
    20461 ttgacaagtt ggaatgatgg agaggggatg tgcaggcggc gtgtgtc
//
LOCUS       DAOTFI010000064        20386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3172_length_20386_cov_6.735429, whole genome shotgun sequence.
ACCESSION   DAOTFI010000064 DAOTFI010000000
VERSION     DAOTFI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 20386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20386
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3172_length_20386_cov_6.735429"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="P5558_08355"
     CDS             complement(<1..185)
                     /locus_tag="P5558_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY24359.1"
                     /translation="MSPTEPMDGQAAGGRFALNVVEFARALRAAGLPVGPGRVLEALR
                     AIEVAGIGSREDLYWTL"
     gene            complement(182..1105)
                     /locus_tag="P5558_08360"
     CDS             complement(182..1105)
                     /locus_tag="P5558_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY24360.1"
                     /translation="MIAHELPKDIDATVELLRAADYVPDRALATATFLSLKLGRPLFL
                     EGEAGVGKTEIAKVLSQALGRDLVRLQCYEGLDLASAVYEWNYQRQMLEIRMAEATGE
                     ASRETLGQDIFDRRFLISRPLLQALEPHAGGAPVLLIDELDRTDEPFEAFLLEVLSDY
                     QISIPEVGTIKAETPPITIITSNRTREIHDALKRRCFYHWVDYPSPEREHEIIRRRLP
                     GVDERLSRSIVDFVQELRKRDLFKLPGVAETLDWTRALTTLDVLVLTPEVINDTLGTL
                     LKYQDDIAKIQGSEAASILREITLKATAARP"
     gene            1164..2369
                     /locus_tag="P5558_08365"
     CDS             1164..2369
                     /locus_tag="P5558_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24361.1"
                     /translation="MNDGIFTFRQAAALYLLCLDHRPSRSLSLDLSIVWRGTTRPPLP
                     RAAAHCVLALAILLIALNLVTFVWLPRYDLVGGQLLVDPGFATLGESRSAWQPVGNSD
                     PPEPVSGGVRLTNDDGQRAVLIEQIVALPAGVEGLRLAGTIELDAVARGPERWQKARL
                     FVLGVKPDGRSDYSRSHRFLHATGTTLPRRWSDVFMLEPARDRARVLIGLPRATGKMV
                     VTDIELQGVALKPAFRLMRNITLAGWVAGALVLAGILWRRSRNRLAATLALGSLAAVI
                     AITAIPYDVRAPVQALFGELGGKGDVGLFKLAMHVGVLALLAIAAGRLLPTLQWSRLW
                     CALVVAGLALELGEWFRGTLDRGDAVDFAANVAGTTLGLSLAFLGERWPARRQRSRRS
                     SGDAAHLSG"
     gene            complement(2447..3241)
                     /locus_tag="P5558_08370"
     CDS             complement(2447..3241)
                     /locus_tag="P5558_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRY24362.1"
                     /translation="MYAFEYKRPASLAEAAQLLSGDDEAKLVAGGQTFIPTLKQRLAQ
                     PSTVIDLGAIGELAGIREEAGGVTIGAMTRHGQVASNDVVRRVIPSLAGMAEVIGDPQ
                     VRNLGTLGGSIANNDPAADYPGALLALQATIRTNKREIAADDFLTGMFDTALEADEII
                     KEVHFKKPDVGHYEKFRNPASRYAIVGAYVTRYGGTVRVAITGAGACAHRWSAAESAL
                     GGNFAADAVAGLTVDAGDLNSDIHASADYRAHLVKVMTQRAVKACA"
     gene            complement(3318..5681)
                     /locus_tag="P5558_08375"
     CDS             complement(3318..5681)
                     /locus_tag="P5558_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY24363.1"
                     /translation="MVANGIGARALRKEDQRFITGKGRYTDDINQHGQAFAVFVRSPH
                     AHAKLVAVDKASVAGMPGVLAVLDGTDVAADGLGNLICGWLVKSKDGSPMKMGAHPLL
                     AQGKVRYVGDRVAVVIAETYAQARDAAEALAVDYAELPATVALADAHGSSTLVHDEVE
                     NNLIFDWELGDKAAVDAAMASAAHVTRIDLVNNRLVPNAMEPRAAIGSYDSGLDQFTC
                     YVTSQNPHVHRLVMSAFIQIAPEHKLRVIGPDVGGGFGSKIFIYGEECVVTWASKKVG
                     GRPVKWVAERSESFLSDAHGRDHISHAELAIDAEGNTTALRVNTKANIGAYMSTFSSC
                     VPTYLYGTLLAGQYKTPAIYCDVQAYYTNTAPVDAYRGAGRPEASFLLETLYEQAAKE
                     IGKDPAEFRRQNFIPKDAFPYQTPVALIYDVGDYATTLDRALELADYAGFKARKAEAE
                     KRGMRRGIGISCYIEACGIAPSAVVGSLGAGVGLWESAKVRFSHTGKVQVMTGTHSHG
                     QGHETTFSQLVADKLGIPYEDIEVIHGDTEKTPVGMGTYGSRSLAVGGEAIAKSCDKL
                     IAKGKKIAAHLLEASEADIEFKDGQFTVAGTDRAKAMGEVVFAAYVPHNYPADLEPGM
                     EEGAFFDPPNFTFPAGTYVCEVEVDPETGVVRIERLVAVDDFGNVINPNIVEGQVHGG
                     LTQGIGQALLESAIYDGAGQLLTGSYMDYAMPRADDVPSFEVETAAGTACTSNSMGVK
                     GCGEAGAIGSPPAVINAIIDAIGVRVEMPATPQRVWAAMQGLALAAE"
     gene            complement(5732..6223)
                     /locus_tag="P5558_08380"
     CDS             complement(5732..6223)
                     /locus_tag="P5558_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY24364.1"
                     /translation="MTKVSMTINGKEMSGEVEGRTLLVDFLRQNLGLTGTHVGCDTSQ
                     CGCCVVHVDGRSVKACTMLAVQANGSKVTTIEGLAAADGALHPVQAAFREHHGLQCGF
                     CTPGMIMSAVDLLQHNANPTEHEVREWLEGNICRCTGYHNIVKAILAAAESMRGGVAH
                     AAE"
     gene            complement(6287..6994)
                     /locus_tag="P5558_08385"
     CDS             complement(6287..6994)
                     /locus_tag="P5558_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HRY24365.1"
                     /translation="MDMTGEYRIPAPREQVWAALNDPEILRQAIPGCDELIKHSDTEL
                     EAKVTAKVGPVKAKFGGNVRLENINPPVGYSIVGEGKGGAAGFAKGGADVSLAEDGDG
                     TILTYSAKAEVGGKLAQIGSRLIQGTAKKMADEFFGKFSELVTAGQAPGAPAAAVGEP
                     PAAAAAPVPEPVGATEAIKMPPPPTAASGPAPRPTPSTAPKPAAGGLLGGLGMPAYAG
                     IAVVVLVLLYLIFGMGG"
     gene            complement(7242..9563)
                     /locus_tag="P5558_08390"
     CDS             complement(7242..9563)
                     /locus_tag="P5558_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY24366.1"
                     /translation="MGAVQSEPGSRVTAGRRELLAAVEQKLLWLASWMIHNANHLRPS
                     RDHLKVGGHQASCASVATIMTALYFDVLRPGDRLAVKPHASPIMHAAHYLLGEQSRAA
                     LENFRSLGGAQSYPSRTKDTAPVDFSTGSVGLGAAMTLFASLTQDYVRLHGLVPEDRP
                     AGRMISIVGDAELDEGNIFEALLEGWKYDVRNLWWVIDYNRQSLDRVVPEQLFAKIEQ
                     FFAAVGWRVVTIKYGKLLQAVFAEAGGAVLRQWIDDCPNDLYAALTFKGGAAWRAHLE
                     SGLGGNRFIRELLRRHDDAGLARLMTNLGGHDIEAVLEAFHSVTDDRPHCFIAYTVKG
                     WGLPFAGHKDNHAGLMTPDQMVEFRSRNRIGDGEEWSPHAGLDIDTAELESFLAAVAY
                     RRRTHRHAPPAVPVPASLPVGRVAATASTQEAFGRIMAELARSDTPLAERIVTTSPDV
                     TVSTNLGGWVTRRGVFNRREHEDVFKSEAVASPTVWRESGRGQHLELGIAENNLFLNL
                     ASLGLAHDLFGARLLPVGTLYDPFINRGLDALIYACYQGARFLLVATPSGITLAPEGG
                     AHQSIGTPLVGMSQPGLTFFEPAYADEVAAIMAWAFRHLQEPDGGSVYLRLSTRQVQQ
                     PRRRLPAGVLDGGYWLAPPGPRTELALVYSGAVVPEVLEAAALLAEDLPELAVLAVTS
                     ADRLHADWRARGKASRAADLLGALPPDVPLVTVLDGHPLALSWLGSVRGQMVTPLGVE
                     RFGQSADIPDLYAAHRLDAAAIVDAAAAALLGA"
     gene            9663..9836
                     /locus_tag="P5558_08395"
     CDS             9663..9836
                     /locus_tag="P5558_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24367.1"
                     /translation="MMTEATVGDAPEEAWRRQVRAIVEELPFDDDLSCFVVALETLAE
                     AFALDDESPEDVA"
     gene            9833..11272
                     /locus_tag="P5558_08400"
     CDS             9833..11272
                     /locus_tag="P5558_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY24368.1"
                     /translation="MSAAELLKGELWTAAAAVRAGEVRARELTEAAIDALTSVGPTLN
                     AVAGLDAEQALAAAERIDRSRAAGTDLPPLAGVPMAHKDMFYRAGRVCASGTRIRAGF
                     VPGVTATALERLDAAGAIDLGRLNMVEFALGLTGHNDITGHPRNPWSTDHITGGSSSG
                     PVAAVAARLVFAALGSDTGGSIRVPAACTGILGLKPTYGRVSRAGALPLSHTLDHVGP
                     LGRSARDLALLMEAVAGVDRRDQTTSTTPVPAYSRLLERSPTRLRLGVAESPFELPLH
                     AEVAGLYDAAVDRMRQAGLRASGLALPALDSLNVLRRVVMIAEAAARHEDFLEERRDA
                     YNAYTLARLDPGFELAATTYLRAVGWRAEAVRRFVGEVFADADVLVLPAMAEPVPRLD
                     ATDTADMAGYVRRVNALGHFICPFNYLGLPALTMPMGRTANGLPMAIQLVAPPFGEAR
                     LLRLAHHLETNAGFRLGEPPIVTGRSGIG"
     gene            11265..12296
                     /locus_tag="P5558_08405"
     CDS             11265..12296
                     /locus_tag="P5558_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRY24369.1"
                     /translation="MADRRQRELRRASDGRLPSRGKVLCASSFLAKRPELLQRLDDEG
                     YEVVFNESGRTLDEAGLGALLPGVVATVAGTEPYNERILAAAPDLRIVARLGVGFDQI
                     DVAAATRYGVAVAMAFGTNHEAVADHTFALMAALASSITSYDRLVRDGSWGSLAHGSL
                     HGTTVGIVGLGRIGRAVAKRCQGFAMRVLAHDPLIDATILGHLGCTQVGLDELLAQAD
                     FVSLHAPLLPATRHLIDAAALSRMKSSAYLINTARGPLVDEAALVAALEAGEIAGAGL
                     DVFESEPLPAGSRLRALESVVLSPHAAGMSEGAVRAMTDRCIDSILAHLDGRPLEPSL
                     ILNPEAAGV"
     gene            12334..13170
                     /gene="map"
                     /locus_tag="P5558_08410"
     CDS             12334..13170
                     /gene="map"
                     /locus_tag="P5558_08410"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRY24370.1"
                     /translation="MKDERTPHLDKGGTGRLVPIHGADDFARMRAAGQLAAMTLDHVT
                     PLVRAGVSTGELDRSIEAFMREHGGVPATLGYRGYTKSSCISVNHVVNHGIPSDDKKL
                     ADGDILNIDVTVILAGFYGDTSRMFAIGERVPVKGQRLIDVTFDAMWAGIDAVRPGAT
                     LGDVGHAIQTVAEAARFSVVRDFVGHGIGRVFHDAPEVRHYGRPGEGLVLAPGMLFTI
                     EPMINAGRHEVKILKDGWTTVTRDRSLSAQFEHTVGVTDDGCEVFTHSPMGFDRPPYA
                     SA"
     gene            complement(13155..14288)
                     /locus_tag="P5558_08415"
     CDS             complement(13155..14288)
                     /locus_tag="P5558_08415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY24371.1"
                     /translation="MHDFGLEEAATPGGFVWQAEHRLIHALDRLPCGVLLADAADRLL
                     HVNSACRKFLPALRDSLTPGTAVSELFRRAVLLGDVRTDAATVDRWLEDRAALDAPSL
                     TLRLRDDRWLKLESCGTFDSGAVLTLIDVTEFKRREVWLTELHDRLAGEGEDLKVFAR
                     HLATAKAEATEALRKAELANQALAREIAERKLLEERLWLLANTDPLTGALNRRRFIEL
                     LEGEARQPDGEPATLAVLMLDLDYFKMINDRFGHATGDAALRHFTEVAAAHLRAPDHF
                     ARLGGEEFAALLPATSIETAVDVAEAIRAAVAAASFEDTGVPVPLSVSIGVTELPPSD
                     GRPLAVLNRADKALYMAKQGGRNRVARVSVGRPPELVEAQAEA"
     gene            complement(14432..16063)
                     /locus_tag="P5558_08420"
     CDS             complement(14432..16063)
                     /locus_tag="P5558_08420"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HRY24372.1"
                     /translation="MEREAMEFDVVIVGAGPSGLAAAIRLAQLAQQHDREVSICVVEK
                     GAEVGAHILSGACFEPRALDELIPDWKDKGAPLHVEATDDQFLYLTETKAYRLPTPPQ
                     MRNHGNYIISLGDLCRWLAEQAEALGVEIYPGFAAAEVLYDESGAVRGVATGDMGVGR
                     DGEPGPNFQPGMELVGRLTLFAEGCRGSLTKTLFERFDLRRDAQPQTFGLGIKELWEI
                     DPAKHEAGRILHTIGWPLDQRTYGGSWLYHFEKNQISIGFVVGLDYTNPHLSPFDEMQ
                     RLKTHPSIRPLLEGGRRIGYGARALSEGGLQSLPGLVFPGGALVGDAAGFLNVPKIKG
                     SHTAMKSGMLAAEAAFEALATDEQRPLLAAYPEKFRASWLHEELHGVRNVRPAFRWGL
                     WPALALNAIDTFVLRGKAPWTLAHHGADHDTLVKAADAPKIAYPKPDGKITFDKLSSV
                     FLSGTNHEEDQPCHLTLADPTVPIRVNLAEYDAPEQRYCPAGVYEIVRDGDEPRLQIN
                     AQNCVHCKTCDIKDPTQNINWVTPEGGGGPMYGSM"
     gene            complement(16139..17260)
                     /locus_tag="P5558_08425"
     CDS             complement(16139..17260)
                     /locus_tag="P5558_08425"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HRY24373.1"
                     /translation="MAQRARPGRKRQAPALAFGLILIVAGSLALFWNEWRSASAVATL
                     VAGERHVVDVPLERVPAGDEGGLLHLAGPARPDGPAVDPLFGIGDLALRLDRKVEMHQ
                     WREIAEKGSANDWTYRYETVWAEGRIPSERFRERLGHVNPPAPTVTSERFYPEGATLG
                     AYGLGRELLDRLPASRPVPIDASALVYLADRELRSTGGALRTGTPGSDRIGDLKVTFS
                     AVPPGEISVLAGLDGSNLRPWQAANGGTVAVARPGLLTAEAMFREVYGMNSRTTWLVR
                     AGATFALVVGYFAAIRPLARLLPPLGVLAHKAGKRIALTLALGHMLVVVALAWVVFRP
                     MAGLALLAVGGGLVTALRRWRALPDDERLGRPPEPPPPG"
     gene            17331..17987
                     /locus_tag="P5558_08430"
     CDS             17331..17987
                     /locus_tag="P5558_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRY24374.1"
                     /translation="MSTLATIDVQMAADRLRRALECLAENDATLRLAIERVGYPAPRI
                     QPAGFATLLRIIVAQQVSTRAAATIQRRVELAMGERVDAQMLLDLPVETLRGCGLSGR
                     KVVYARELAAAIVDGRLDLERLAAAPDEEVVSALTALPGFGRWSAEIYLLFALQRQDV
                     LPADDLALQIAAQRLKNLEVRPNARDLRALAVAWSPWRGAASVFLWHYYGAATLDSVA
                     "
     gene            18162..>20386
                     /gene="gyrA"
                     /locus_tag="P5558_08435"
     CDS             18162..>20386
                     /gene="gyrA"
                     /locus_tag="P5558_08435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRY24375.1"
                     /translation="MKRSYLDYAMSVIVSRALPDVRDGLKPVQRRILYAMRVAGYDIG
                     RPHRKSALIVGDVMGKYHPHGDGAIYDAMVRMAQHWSMRLMLIDGQGNFGSMDNDPPA
                     AMRYTEARLARPAAALLDDIDKDTVGFRPNYDESISEPEVLPAQYPNILVNGAGGIAV
                     GMATNIPPHNLGEIIDATIALIDDPELDIADLMEIVQGPDFPTGGIILGRAGIQQAYM
                     TGRGSLTVRSRCTIEEIRKDRQAILVHEVPYLVNKARLVERIAEVVREKRVEGISDLR
                     DESDRHGVRVVIEIKRDADAEVVLNQLYKFTPLQTGFGINMVALNNGRPMQMTLVEVL
                     QAFIAFREDVILRRTAYDLAKARERAHILVGLAVAVANIDEVIALIRAAPDPQTAKAG
                     LLERAWPAEAILPLLALVEDGTAAATEYRLSERQAQAILDLRLQRLTGLEREKITAEL
                     AEIAGAIKELLLILQSRERLLEVMKAELTDVRSRFADERRTFIDLDAELDIDIEDLIP
                     REDMVVTVTHKGYIKRVPLSTFRAQRRGGKGRAGMRTHEDDFVARLFVASTHTPVLFF
                     SSLGRVYKLKVYKLPLGNPQARGKAMINLFPHLVEGEWITAILPLPEDEATWVELSAV
                     FATARGKVRRNDLADFTRVPVNGKIAMGLDDGDRLIGVDVCDDSHDVLLVTGGGKAIR
                     FAVESLRVFRSRTSEGVRGIDLAGEDAVISMSILDHITLSTEERDELVRIANARRRAA
                     AL"
BASE COUNT         3102 a         6885 c         7098 g         3301 t
ORIGIN      
        1 tgcagggtcc agtagagatc ctcgcggctg ccgatgccgg ccacctcgat cgccctgagc
       61 gcctcgagca cccgcccggg accgaccggc aggccggcgg cgcgcagcgc ccgggcgaac
      121 tcgacgacgt tgagcgcaaa acgtccgcct gccgcctgac catccatcgg ctcggtcggg
      181 ctcatggccg ggcggcagtt gccttgaggg tgatctcgcg gaggatgctg gcggcttcgc
      241 tgccctggat cttggcgatg tcgtcctgat acttgaggag cgtgccgagc gtgtcgttga
      301 tgacctcggg cgtcagcacc aggacgtcga gtgtggtcag ggctcgcgtc cagtcgagcg
      361 tctcggccac gcccggcagc ttgaagaggt cgcgcttcct gagctcctgc acgaagtcga
      421 cgatcgagcg cgacagccgc tcgtcgacac cgggcagccg ccgcctgatg atctcgtgct
      481 cgcgctcggg tgaggggtag tcgacccaat gatagaagca gcgacgcttc agcgcgtcgt
      541 ggatctcgcg ggtccggttc gaggtgatga tggtgatcgg cggtgtctcg gccttgatcg
      601 tgccgacctc gggaatgctg atctggtagt ccgagagcac ctcgagcagg aacgcttcga
      661 acggctcgtc ggtgcggtcg agctcgtcga tcagcagcac gggtgccccg cccgcatgcg
      721 gctccagcgc ctgcaggagc gggcggctga tcaaaaagcg ccggtcgaag atgtcctggc
      781 ccagcgtctc gcgagaagcc tcgccggtcg cctccgccat gcggatctcg agcatctgcc
      841 gctggtagtt ccattcatag acggcggatg ccaggtcgag gccctcgtag cattgcaggc
      901 ggacgagatc ccgccccagc gcctggctca gcaccttggc gatctcggtc ttgccgacgc
      961 ccgcctcgcc ctcgaggaag agtggccggc cgagcttgag actgagaaag gtcgccgtgg
     1021 cgagcgctcg atcggggaca tagtcggcgg cacgcagcag ctcgaccgtg gcgtcgatat
     1081 ccttcgggag ttcgtgtgcg atcatttccg tttctcgagg ctggacggtc gaccgtttac
     1141 ccgctgggcc ggccgatggc tacgtgaacg atgggatctt cacctttcgg caagcagcag
     1201 cgctgtatct cctctgcctg gatcatcggc cgtcccggag cctgtcgctg gacctgtcga
     1261 tcgtttggag gggaacaacg cgcccgccgc tgccgcgagc agccgcgcat tgcgttctgg
     1321 cgctcgcgat cctgttgatc gcgttgaatc tcgtgacctt cgtctggctg ccgcgctacg
     1381 atctggtcgg tggccagttg ctcgtggatc cgggtttcgc gacgctgggc gagagccggt
     1441 cggcctggca gcccgtgggc aactccgacc caccggaacc ggtttccggc ggcgtgcggc
     1501 tgacgaacga cgacggccaa agggccgtcc tcatcgagca gattgttgca ctcccggcgg
     1561 gtgtcgaggg gcttcgtctt gccggcacca tcgagctcga cgctgtcgcc cgcggccccg
     1621 aacgctggca gaaggcccgg ctcttcgtgc tcggcgtgaa acccgatggg cgttccgact
     1681 attcccgctc gcatcgtttc ctccatgcga ccggaacgac cctccccaga cgctggagcg
     1741 acgtcttcat gctcgagccg gcccgcgacc gggcacgggt gctgatcggg ctgccccggg
     1801 ccactggtaa gatggtcgtc accgatatcg agctgcaggg cgttgccctg aaaccggctt
     1861 tcaggctgat gcgcaacatc actctcgccg gctgggtggc cggtgcgctg gtgcttgccg
     1921 gcattctctg gcggcgcagt cgcaacaggc ttgccgccac gctggcgctg ggctcgctgg
     1981 cggccgtcat cgccatcacc gcgatccctt acgacgttcg ggcacctgtt caagcgctct
     2041 tcggcgagct gggcggcaaa ggcgacgtcg ggctcttcaa gctcgcgatg catgtcggcg
     2101 tgctcgccct ccttgccatc gccgcagggc ggctgttgcc cacgctgcaa tggagccggc
     2161 tctggtgcgc tctggtcgtt gccgggctgg ccctggagct cggcgagtgg ttcaggggca
     2221 cgctcgatcg cggtgatgcc gtggatttcg ccgccaatgt cgctggcacg acgctgggtc
     2281 tgagcctcgc atttctgggc gagcgctggc cggcgcgccg ccaacggtca aggcggagct
     2341 ccggcgacgc tgcgcacttg tccggatagc ggggctgcgg cggcaacgat gccgacctgg
     2401 tcgcggcagt cccgccgcga ccagatgtgc catcgctcgt tcagtctcag gcgcaggcct
     2461 tgaccgcgcg ctgcgtcatc accttgacga ggtgggcgcg atagtcggcc gaggcatgga
     2521 tgtccgaatt gaggtccccg gcatccacgg tcaggccggc cacggcatcc gccgcgaaat
     2581 tgcccccgag cgcactctcg gcggccgacc agcgatgcgc gcaggcgccg gcaccggtga
     2641 tggcgacccg cacggtccca ccgtacctgg tcacataggc accgacgatg gcatagcgcg
     2701 aggcggggtt ccggaacttc tcgtaatgcc cgacatccgg cttcttgaaa tgcacttcct
     2761 tgatgatctc gtccgcctcg agggccgtgt cgaacatgcc tgtgaggaag tcgtcggccg
     2821 cgatctcgcg cttgttcgtg cggatcgtcg cctgcagcgc caggagcgcg cccgggtagt
     2881 cggcggcggg gtcgttgttg gcgatcgagc cacccaacgt gcccaggttg cgcacctgcg
     2941 gatcaccgat cacttcagcc atgcccgcaa gcgatggaat cacccgccgg acaacatcgt
     3001 tcgatgcgac ctggccgtgg cgggtcatgg cgccaatggt caccccgccg gcttcctcgc
     3061 ggatgccggc gagctcgccg atggcgccca gatcgatgac ggtactgggc tgggcgagac
     3121 gctgcttgag ggtcgggatg aaggtctggc ccccggccac cagcttcgcc tcgtcatcgc
     3181 cgctcagcaa ctgcgcggcc tcggcgaggg aagcgggccg cttgtactcg aaagcataca
     3241 tcgttcggtt tctcccttgc gggcgagggg ttggcccgtg gcccgacccc gcccggtttg
     3301 ctgtgtcctg aaatcgccta ctcggccgcc agcgccagcc cctgcatggc cgcccagacc
     3361 cgttgcggcg tcgccggcat ctcgacgcgc acgccgatcg cgtcgatgat ggcgttgatc
     3421 accgccggcg gcgagccgat ggcgccggcc tcgccgcagc ccttgacccc catcgagttg
     3481 gaggtgcagg cggtgccggc ggcggtctcc acctcgaagc tggggacgtc gtcggcccgc
     3541 ggcatggcgt agtccatgta ggagccggtg aggagctggc cggcaccgtc gtagatggca
     3601 ctttcgagca aggcctggcc gatcccctgg gtcaggccgc catggacctg gccttcgacg
     3661 atgttcgggt tgatcacatt gccgaaatcg tcgaccgcca cgagccgctc gatgcggacg
     3721 acgccggtct cgggatcgac ctcgacctcg cacacatagg tgccggccgg gaaggtgaag
     3781 ttcggcgggt cgaagaaggc cccttcctcc atccccggct ccaggtcggc cgggtagttg
     3841 tgcggcacgt aggcggcgaa gaccacctcg cccatcgcct tggcccggtc ggtgccggcg
     3901 acggtgaact gcccgtcctt gaactcgatg tccgcttccg aggcctcgag cagatgggct
     3961 gcgatcttct tgcccttggc gatcagcttg tcgcacgact tggcgatcgc ctcgccgccg
     4021 acggcgagcg agcgcgagcc ataggtgccc atgccgaccg gcgtcttctc ggtatcgccg
     4081 tggatgacct cgatatcctc gtaaggaatg ccgagcttgt cggcgacgag ctggctgaag
     4141 gtcgtctcgt ggccctggcc gtggctgtgg gtgccggtca tcacctggac cttgccggta
     4201 tggctgaagc ggactttcgc actctcccag agcccgacgc ccgcaccgag cgagccgacc
     4261 actgccgagg gtgctatgcc gcaggcctcg atgtagcagg agatgccgat gccacgtcgc
     4321 atgccgcgct tctcggcctc ggccttgcgt gccttgaacc cggcatagtc ggcgagctcc
     4381 agtgcgcgat ccagggtcgt cgcatagtcg ccgacatcgt agatcagggc gaccggcgtc
     4441 tggtagggga aggcatcctt gggaatgaag ttctgccgcc ggaactcggc ggggtccttg
     4501 ccgatctcct ttgccgcctg ctcgtagagc gtctccagca ggaaggaggc ctcgggccgg
     4561 ccggcaccgc gataggcgtc cacgggcgcc gtgttcgtgt agtaggcctg cacgtcgcaa
     4621 tagatcgccg gcgtcttgta ctgaccggcg aggagcgtgc cgtagagata ggtcggcacg
     4681 cacgagctga aggtcgacat gtaggcgccg atattcgcct tggtgttgac cctgagtgcc
     4741 gtagtgttgc cctcggcgtc gatcgccagc tcggcatggc tgatgtgatc gcggccatgg
     4801 gcatcggaaa ggaagctttc gctgcgttcg gcgacccatt tgaccggccg gccgccgacc
     4861 ttcttcgatg cccaggtgac gacgcattcc tcgccgtaga tgaagatctt ggagccgaag
     4921 ccgccgccga cgtccgggcc gatcacccgg agcttgtgct cgggtgcgat ctggatgaag
     4981 gcggacatga ccagccggtg gacatgcggg ttctggctgg tgacgtagca ggtgaactga
     5041 tcgaggccgc tgtcatagga gccgatcgcc gcgcggggct ccatggcgtt gggcaccagg
     5101 cggttgttga ccagatcgat gcgggtgaca tgcgcggccg aggccatggc cgcgtcgacc
     5161 gccgccttgt cgccgagctc ccagtcgaag atcaggttgt tctcgacctc gtcatggacc
     5221 agcgtcgacg agccgtgcgc gtcggcgagc gccacggtcg ccggcagctc ggcgtagtcg
     5281 accgcgagcg cttcggccgc gtccctggcc tgggcatacg tctcggcgat gaccacggcg
     5341 acccggtcgc cgacataacg gacctttccc tgtgccagga gcgggtgggc gcccatcttc
     5401 atgggcgagc cgtccttcga tttgaccagc cagccgcaga tcaggttgcc gaggccgtca
     5461 gccgcgacat ccgtcccgtc gagcacggcc agcacgccgg gcatgccggc gacgctggcc
     5521 ttgtcgacag ccaccagctt ggcgtgggca tgcgggctgc gcacgaacac ggcaaaagcc
     5581 tggccgtgct ggttgatgtc gtcggtatac cggcctttgc cggtgatgaa gcgctggtct
     5641 tccttgcgga gtgcccgagc accgatgccg ttagccacca tcttttgctt ccctgttgac
     5701 gaagcggccc ccgtgacccg tcctgttctc gttattccgc ggcgtgcgcg acgccgcccc
     5761 gcatggactc ggcagccgcg aggatcgcct tcacgatgtt gtggtagccg gtgcagcggc
     5821 agatattgcc ctcgagccac tcccgaacct cgtgctcggt gggattggcg ttgtgctgca
     5881 ggaggtcgac ggccgacatg atcatgccgg gcgtgcagaa accgcactgc aggccgtgat
     5941 gctcgcggaa ggcggcctgg accgggtgca gggcgccatc cgcggcggcc agcccctcga
     6001 tggtcgtcac cttgctgcca ttcgcctgaa cagccagcat ggtgcaggcc ttcacgctgc
     6061 ggccgtcgac atggacgacg cagcagccgc actgcgaggt gtcgcagccg acatgcgtgc
     6121 ccgtcaggcc gagattctgc cgcaggaaat cgacgagcag cgtgcgcccc tcgacctcgc
     6181 cggacatctc cttgccgttg atcgtcatcg aaaccttggt cattctctca atccctccca
     6241 gcggcgacca cctggtcgct gcaccgttgc gaactcgagc ggctcgtcag ccgcccatac
     6301 cgaagatcag ataaagcagg accagcacga ccacggcgat acccgcatag gccggcatgc
     6361 cgaggccgcc gagcaaaccg ccagcggcag gcttcggggc ggtgctcggt gtcggacgcg
     6421 gggcgggtcc gctcgcggca gtcggcggtg gcggcatctt gattgcttcc gtggcaccca
     6481 ccggctcggg cacaggtgca gccgctgcgg ccgggggttc gccaacggcc gctgccggcg
     6541 cgccgggcgc ctgacccgcg gtcacgagct cgctgaactt gccgaaaaac tcgtcggcca
     6601 tcttcttggc cgtgccctgg atcagccgcg agccgatctg cgccagcttg ccaccgacct
     6661 cggccttggc gctgtaggtc aggatcgtgc cgtcgccatc ctcggcgagg ctcacgtcgg
     6721 cgccaccctt ggcgaaaccg gcggcaccgc ccttgccctc accgacgatg ctgtagccca
     6781 ccggcgggtt gatgttctcg aggcgcacat tgccaccgaa cttcgccttc accggaccga
     6841 ccttggcggt caccttggcc tcgagctcgg tatccgagtg cttgatcagc tcgtcgcagc
     6901 cggggatcgc ctggcggagg atctcgggat cgttgagcgc cgcccagacc tgctcacgcg
     6961 gcgccggtat ccgatattcg cccgtcatat ccatgcgcgc actccccgtt ctcttcggcc
     7021 cggtcggatc gggtgggcac caatgcggcc cagccgcgaa gctcgatcgg gcaatggctt
     7081 gcttcgccgt ccaagggtta tggcgaaacc gcgccgagcc gacaaggccg gccgcatgga
     7141 tggcgccccc gtccaccacc atcgcccgcg agccggccgc cgcagccgac cagcagggcg
     7201 agaaaatcgt cggtggccag atgtcgacgc gtggccactg cctaggctcc aagcagcgcc
     7261 gcagcggcgg catcgacgat tgccgcggcg tcgagacggt gcgcggcgta caaatccgga
     7321 atgtcggccg actggccgaa ccgctcgacg ccgagcggcg tcaccatctg gcccctgacc
     7381 gagccgagcc aggaaagggc taggggatgg ccgtcgagca cggtgaccag cggcacgtcc
     7441 ggcggcaggg cgccgagcag gtcggcggcg cgactggcct tgccccgtgc ccgccagtcg
     7501 gcatgcagtc gatcggccga ggtgacggcg agcacggcca gctccggcag atcctccgcg
     7561 agcagtgccg ctgcttccag cacttcgggc acgaccgcac ccgaatagac gagcgccagc
     7621 tcggtccgcg gcccgggtgg tgcgagccag tagccgccgt cgaggacccc tgccggcagc
     7681 ctgcgccggg gctgctggac ttgccgggtc gagagccgga gatagaccga gccgccatcg
     7741 ggctcctgca ggtgccgaaa agcccaggcc atgatcgccg ccacctcgtc ggcgtaggcc
     7801 ggctcgaaga aggtcaggcc cggctgcgac atgccgacca gcggcgtgcc gatcgactgg
     7861 tgcgcaccac cctccggcgc cagcgtgatg cccgagggcg tcgccacgag gaggaagcgg
     7921 gcgccctggt agcaggcgta gatcagggca tcgagaccgc ggttgatgaa gggatcgtag
     7981 agtgtgccga ccggcaggag ccgggcgccg aagagatcgt gggcgaggcc caggctggcg
     8041 aggttcagga agaggttgtt ctcggcgatg ccgagctcga ggtgctggcc acgcccgctt
     8101 tcccgccaca cggtgggcga ggccacggcc tcgctcttga agacgtcctc gtgctcgcgg
     8161 cgattgaaga cgccgcgccg cgtgacccag ccgccgagat tggtggagac ggtcacgtcg
     8221 ggcgaggtcg tgacgatgcg ctcggcgagc ggcgtgtcgg agcgcgcgag ctcggccatg
     8281 atccgcccga aggcctcctg ggtcgaggcc gtggcggcga cccggccgac cggcagggat
     8341 gccggcacgg gcacggctgg cggcgcgtgg cggtgggtcc gccggcggta ggcgacggcg
     8401 gcgaggaagc tctcgagctc ggccgtgtcg atgtcgagcc cggcatgggg cgaccattcc
     8461 tcgccgtcgc cgatccggtt gcgcgagcgg aactcgacca tctggtcggg cgtcatcagc
     8521 ccggcatggt tgtccttgtg cccggcgaag ggcaggcccc agcccttgac cgtgtaggcg
     8581 atgaagcagt gcgggcggtc atcggtgacc gaatggaacg cctcgagcac ggcctcgatg
     8641 tcgtggccgc cgagattggt catgagacgg gccagcccgg cgtcgtcatg acggcgcagg
     8701 agctcgcgga tgaagcggtt gccgccgagg ccgctctcca ggtgcgcccg ccaggcggcg
     8761 ccacccttga aggtgagggc ggcgtagaga tcattggggc aatcgtcgat ccactggcgc
     8821 agcaccgcgc cgcccgcctc ggcgaagacg gcctggagca gcttgccgta cttgatcgtg
     8881 acgacccgcc agccgaccgc cgcgaagaac tgctcgatct tggcgaagag ctgctccggg
     8941 accacgcggt ccaggctctg gcgattgtag tcgatcaccc accagagatt gcggacatca
     9001 tatttccagc cctcgaggag ggcctcgaag atattgccct catcgagctc ggcatcgccg
     9061 acgatcgaga tcatgcggcc ggccggccga tcctccggca cgagcccgtg caggcgaacg
     9121 tagtcctggg tgagcgacgc gaacagggtc atcgcggcgc cgaggccgac cgagcccgtc
     9181 gagaagtcga ccggcgcagt gtccttggtc cgcgagggat aggactgggc accgccgagc
     9241 gagcggaagt tctcgagcgc ggcccggctc tgctcgccga gcaggtagtg ggcggcgtgc
     9301 atgatggggc tggcatgcgg cttgacggcg agtcgatcgc cgggtcgcag cacgtcgaaa
     9361 tagagcgccg tcatgatcgt cgccacggat gcgcaggatg cctgatgacc acctaccttc
     9421 aggtgatcgc ggctcggccg gaggtggttg gcgttgtgaa tcatccagct cgccagccac
     9481 aggagcttct gctccaccgc ggcgagcagc tcccgccggc cggcggtgac gcgcgagccc
     9541 ggctcggatt ggacagcccc catgacattc tcctgcgcaa gcggcggcca gtcttgcgct
     9601 atcgggatgc atcggcaagc accgccagct aagcagccgc cttatcgtgg cacgaggggg
     9661 acatgatgac cgaagcgaca gtcggggacg cgccggagga agcctggcgc aggcaggttc
     9721 gcgccattgt cgaggagctg cccttcgacg acgacctgtc ctgtttcgtc gtcgccctcg
     9781 agacgctggc cgaggctttt gccctggacg acgagtctcc ggaagacgtg gcatgagtgc
     9841 ggcggagctg ctcaagggtg agctctggac ggcagccgcg gcggtccggg cgggcgaggt
     9901 ccgggcccgc gagctgaccg aggccgcgat cgacgcgctg acgagcgtgg gccccacgct
     9961 caacgcggtc gccgggctcg acgccgagca ggcgctggcg gcggccgaac gcatcgatcg
    10021 gtcccgcgct gccgggaccg acctgccgcc attggctggc gtcccgatgg cgcacaagga
    10081 catgttctac cgtgccggcc gcgtttgcgc ctctggaacc cggatccgtg cgggctttgt
    10141 gcccggggtc accgccaccg cgctcgagcg gctggacgcc gccggtgcga tcgacctcgg
    10201 tcggctcaat atggtcgagt tcgccctggg gctcacgggc cacaacgaca tcaccggcca
    10261 tccgcggaac ccatggagca ccgaccacat caccggcggc tcgtcgagcg ggcccgtggc
    10321 ggccgtggcg gcgcggctgg tcttcgcggc cctaggctcc gacaccggcg ggtcgatccg
    10381 ggtgcccgcc gcgtgcaccg gcattctcgg gctcaagccg acctacgggc gagtcagccg
    10441 ggcgggtgcc ctgccgctca gccatacgct cgaccatgtc ggcccgctcg gccgctcggc
    10501 gcgcgacctg gcgctgctga tggaagcggt tgccggcgtg gatcgccgtg atcagacgac
    10561 cagcacgacg ccggtgccag cctacagcag actcctcgag cgctctccga cgcgcctgcg
    10621 cctcggcgtc gccgaatccc ccttcgagct gccgttgcac gcggaggtgg ccgggctcta
    10681 cgacgcggcg gtcgatcgca tgcggcaggc ggggctgcgc gccagtgggc tggccctgcc
    10741 cgcgctcgat tccctgaacg tgctgcggcg agtcgtcatg atcgcggagg ctgcggcaag
    10801 gcacgaggat tttctggagg agcggcgtga cgcctacaac gcctacaccc tggcgcgcct
    10861 agatccgggg ttcgagctcg ccgcgacgac ctatctcagg gcggtaggct ggcgggccga
    10921 ggcggtgcgg cggttcgtcg gagaggtctt cgccgatgcc gatgtgctgg tgctgccggc
    10981 catggccgag ccggtgcccc ggctggatgc aaccgatacg gcggacatgg ccggctatgt
    11041 ccgaagggtc aacgcgctcg gccacttcat ctgcccgttc aactatttgg ggctgcccgc
    11101 cctgacgatg cccatgggtc gcacggcgaa cggcctgccg atggcgatcc agctcgtggc
    11161 cccgccgttc ggcgaggcgc gcctgctgcg gctggctcat catctggaga cgaatgccgg
    11221 cttccgcctc ggcgagccac ccatcgtcac cgggcgttcc ggcattggct gaccgtcggc
    11281 agcgcgagct gcgccgcgcc agcgacgggc ggctgcctag ccggggcaag gtgctctgtg
    11341 cgtcgtcgtt tctcgcgaag cgcccggagc tcctgcaacg gctggacgac gagggctacg
    11401 aggtcgtctt caacgagtcc gggcggacgc tcgacgaggc ggggcttggc gcactcctgc
    11461 ccggtgtcgt cgccaccgtc gccggcaccg agccctacaa cgagcggatt ctcgccgcgg
    11521 cacccgatct gcggatcgtg gcgcggctgg gggtcggctt cgaccagatc gatgtggcgg
    11581 ccgccacccg gtacggggtg gcggtggcga tggcgttcgg caccaatcac gaggcggtgg
    11641 ccgatcacac cttcgccctg atggcggcgc tcgcctcgtc gatcacgagc tacgatcgac
    11701 tcgtgcggga cggctcgtgg ggcagcctgg cgcacggcag cctgcacggc acaacggtcg
    11761 gcattgtcgg ccttggccgc attggccggg cggtggccaa gcgctgccag ggcttcgcca
    11821 tgcgggttct ggcgcacgat ccgctgatcg atgcgaccat cctcggccat ctcggctgca
    11881 cgcaggtggg cctcgacgag cttctggccc aggccgattt cgtctcgctg cacgccccgc
    11941 tcctgccggc gacgcgccac ctgatcgacg cggcggctct ttcgcgaatg aagagcagcg
    12001 cctatctgat caacacggca cgcggtcccc tggtcgatga ggcggcgctg gttgccgcgc
    12061 tcgaggctgg cgagatcgcg ggcgccggac tcgatgtctt cgagagcgag ccgctgccgg
    12121 cgggctcgcg gctgcgtgcg ctcgaatcgg tggtattgtc gccgcacgcg gccggcatga
    12181 gcgagggggc ggtccgagcc atgaccgaca ggtgcatcga cagcatcctg gcgcacctcg
    12241 atggccggcc gctggagccg agcctgatcc tcaatccgga ggcggcaggc gtgtgaggca
    12301 agacgagggt caagcgatgc aggtggacga gcgatgaagg acgaacgaac gccgcacctg
    12361 gacaagggcg gcaccggccg gctcgtgccg atccacggtg ccgacgactt cgcccggatg
    12421 cgtgcggccg gccagctcgc ggcaatgacg ctcgatcacg tgacgccgct ggtccgggcc
    12481 ggcgtgagca ccggcgagct cgaccgttcg atcgaggcct tcatgcgcga gcatggcggc
    12541 gtgccggcga cgctgggcta tcgcggctac accaagagct cgtgcatctc ggtcaaccac
    12601 gtcgtcaatc acggcatccc gagcgacgac aagaagctcg cggatggcga tatcctgaat
    12661 atcgacgtca ccgtgatcct cgccgggttc tatggcgaca cgagccgcat gttcgcgatc
    12721 ggcgagcggg tgccggtcaa ggggcagcgg ttgatcgacg tcaccttcga tgcgatgtgg
    12781 gccggcatcg acgcggtgcg gccgggagcc accctgggcg atgtcggcca tgccatccag
    12841 acggtggcgg aggctgcgcg cttctcggtg gtccgggact tcgtcgggca cgggatcggg
    12901 cgtgtcttcc acgatgcgcc ggaggttcgc cattatggtc ggccgggcga ggggctggtc
    12961 ctggcgccgg gcatgctctt cacgatcgag ccgatgatca atgccgggcg ccacgaggtg
    13021 aagatcctca aggacggctg gacgacggtc acccgggatc gctcgctctc ggcccagttc
    13081 gagcacacag tcggcgtcac ggatgatggc tgcgaggtct tcacccactc gccgatgggc
    13141 ttcgaccgcc caccctacgc ctcggcctga gcttcgacca gctccggcgg tcggccgacg
    13201 ctgaccctgg cgacccggtt gcggccgccc tgcttcgcca tatagagcgc cttgtcggca
    13261 cggttgagca ccgcgagcgg ccggccgtcc gacggcggca gctccgtgac gccgatgctg
    13321 acgctcaaag gcacgggaac accggtgtcc tcgaagctcg ccgctgcgac cgcggcgcgt
    13381 atcgcctcgg cgacgtcgac cgccgtctcg atcgatgtcg ccggcagcag ggccgcgaac
    13441 tcctcgccgc ccagccgggc gaagtggtcc ggcgcgcgca ggtgcgccgc cgccacctcg
    13501 gtgaagtgac gcaacgccgc gtccccggtc gcgtgaccga agcggtcgtt gatcatcttg
    13561 aagtagtcga gatcgagcat cagcacggcc agcgttgccg gttcgccatc cggctgccgt
    13621 gcctcgcctt ccagcaactc gatgaagcgc cggcgattga gcgccccggt cagtggatcg
    13681 gtgttggcga gaagccagag ccgctcctcc aggagcttgc gctcggcgat ctcgcgtgcc
    13741 agggcctggt tcgccagctc cgccttcctg agcgcctcgg tcgcttccgc cttggcggtc
    13801 gccagatgac gggcgaagac cttgagatcc tcgccctcgc cggcgagccg gtcgtgcagc
    13861 tcggtgagcc agacctcgcg acgcttgaac tcggtgacat cgatcagcgt caggacggca
    13921 ccactgtcga acgtgccgca cgattccagc ttgagccagc gatcgtcgcg cagccggagc
    13981 gtcaggctcg gcgcgtcgag cgcggcgcga tcctcgagcc agcgatcgac ggtcgccgca
    14041 tcggtccgca cgtcaccgag cagcacggcc cggcgaaaga gctcggacac ggcagtgccc
    14101 ggggtgaggg aatcgcgcag ggccggaagg aatttgcgac acgccgagtt gacgtgcagg
    14161 agccggtcgg cggcatcggc caggagcacg ccgcagggca accggtcgag cgcatggatg
    14221 agccgatgct cggcttgcca gacgaagccg cccggcgtcg cggcctcctc cagaccgaag
    14281 tcatgcatct tggcagtgta cctctacggg acccgggccg atcagggctc gggaggacag
    14341 tcttgacctg tttaccacaa gcctacttgc ggttgaagta tattgccgcc caccatcgca
    14401 tgtgagactg ccggatgcag gccccgaaac ctcacatgct gccgtacatc ggaccgccgc
    14461 cgccctccgg tgtcacccag ttgatgttct gcgtcgggtc cttgatgtcg caggtcttgc
    14521 agtgcacgca gttctgggcg ttgatctgga ggcgcggctc gtcgccgtca cggacgatct
    14581 cgtagacgcc cgccgggcag tagcgctgct ccggtgcgtc gtactcggcg aggttgaccc
    14641 ggatcggcac tgtcggatcg gccagggtga gatggcaggg ctggtcctcc tcgtggttgg
    14701 tgccggagag gaagaccgag ctcagcttgt cgaaggtgat cttcccgtcc ggcttcgggt
    14761 aggcgatctt cggcgcgtcc gcggctttca ccagggtgtc gtggtcggca ccgtgatgcg
    14821 ccagcgtcca gggcgccttg ccgcgcaaga cgaaggtgtc gatggcattg agcgccaaag
    14881 cgggccagag gccccacctg aacgccggcc gcacattgcg cactccgtgc agttcctcat
    14941 ggagccagct cgcacggaac ttctcgggat aggcggcgag cagcggccgc tgctcgtccg
    15001 tcgccagcgc ctcgaaggcg gcctcggccg cgagcatgcc ggatttcatc gccgtatggc
    15061 tgcccttgat cttcggcacg ttgaggaagc cggccgcatc gccgacgagg gcaccgcccg
    15121 ggaagacgag ccccggcagg gactgcagcc cgccctcgga gagggcccgg gcgccgtagc
    15181 cgatccgccg tccgccctcg agcaacggcc ggatcgacgg gtgcgtcttc agccgctgca
    15241 tctcgtcgaa cggggaaaga tgcgggttgg tgtagtcgag gccgacgacg aagccgatgg
    15301 agatctggtt cttctcgaaa tggtagagcc aggagccgcc ataggtgcgc tggtcgagcg
    15361 gccagccgat ggtgtgcagg atccggcccg cctcgtgctt cgcgggatcg atctcccaga
    15421 gctccttgat gccgagcccg aaagtctgcg gctgggcgtc ccggcggaga tcgaagcgct
    15481 cgaacaatgt cttggtcagc gacccgcggc agccttcggc gaagagcgtg aggcgaccga
    15541 ccagctccat gccgggctgg aagttcggcc cgggctcgcc gtcgcggccg acgcccatgt
    15601 cgcccgtcgc cacgccccgc acggcgccgc tctcgtcgta gagcacctcg gcggcggcga
    15661 agcccggata gatctcgacg ccgagcgcct cggcctgctc ggcgagccag cggcagagat
    15721 cgccgaggct gatgatgtag ttgccgtgat tgcgcatctg cggcggcgtc ggcaggcggt
    15781 aggctttggt ctcggtgagg tagaggaact ggtcgtccgt tgcctcgacg tggagtggcg
    15841 cacccttgtc cttccagtcg gggatcagct cgtccagtgc gcgcggctcg aagcaggcgc
    15901 ccgagagaat atgggccccg acctccgcac ccttctcgac gacacagatg gagacctcgc
    15961 ggtcgtgctg ctgcgccagc tgcgccaggc ggatggcggc ggcgaggccg ctcggaccgg
    16021 ccccgacgat caccacgtcg aactccatcg cctcgcgttc caagactctc actcctctgc
    16081 ggcaaacaag cggtcggcac aggttatcta gaggccgtgc ggccataagg ccatggggtc
    16141 agccgggcgg cggcggctcg ggggggcggc cgaggcgctc gtcatctggc agggcgcgcc
    16201 aacggcgaag ggccgtcacg agcccgccgc cgaccgcgag cagcgccagc ccggccattg
    16261 gacgaaagac gacccaggcc agggcaacga ccaccagcat gtggccgagc gcgagcgtca
    16321 gggcgatccg cttcccggcc ttgtgcgcga gcacacccag aggcggcagc aatcgggcca
    16381 gcgggcggat cgcagcgaaa tagccgacaa cgagcgcgaa ggtggcgccg gcccgaacga
    16441 gccaggtcgt ccggctgttc atgccgtaca cctcgcgaaa catggcctcg gcggtcagca
    16501 ggcccgggcg ggccacggcg acggtgccgc cgttggctgc ctgccaggga cgcaggttgg
    16561 agccgtcgag accggccagc acgctgatct cgccgggcgg gacggcgctg aaggtgacct
    16621 tcaggtcgcc gatccggtcc gagcccggcg tcccggtccg cagcgccccg ccggtcgagc
    16681 gcagctcccg atcggcgagg tacaccaggg cactggcgtc gatcggaacc ggccggctcg
    16741 ccgggagccg gtcgaggagc tcccggccga gcccgtaggc gccgagcgtc gcaccctccg
    16801 gatagaagcg ctcgctcgtg acggtcgggg ccggcgggtt gacgtggccg agtcgctcgc
    16861 gaaagcgctc ggacgggatt cgcccctcgg cccagaccgt ctcgtagcgg taggtccagt
    16921 cattggccga acccttctcg gcgatctccc gccactgatg catctcgacc ttgcgatcga
    16981 gacgcagggc gaggtcgccg atgccgaaga gcggatcgac tgccggcccg tccggccggg
    17041 ccgggccggc gagatgcagg agcccgccct cgtcgccggc ggggacccgc tcgagcggga
    17101 cgtcgacgac gtgccgctcg ccggcgacca gcgtcgccac ggcggaggcc gaacgccact
    17161 cgttccagaa gagtgctaag ctgccggcga cgatcaggat caggccgaag gccagtgccg
    17221 gcgcctggcg ctttcttcct ggccttgccc gttgcgccac ctgtggctct ccctcgacga
    17281 gaatgatggc aagcttgccg agaccggacc gatcgggcaa ggcatcgcgg atgtcgaccc
    17341 tggcgacgat cgatgtgcag atggcggccg accgcctgcg gcgtgcgctc gaatgccttg
    17401 ccgagaacga tgcgacgctc cggctcgcca tcgagcgggt cggctatccg gcacctcgga
    17461 tccagcctgc cggctttgcc accttgctgc ggatcatcgt cgcccagcag gtgtccacgc
    17521 gggccgcggc caccatccag cgccgcgtcg agttggcaat gggcgagcgg gtggatgcgc
    17581 agatgctgct ggatctgccg gtcgagacgc tgcgcggctg cgggctcagc gggcgcaagg
    17641 tggtgtatgc gcgagaactg gcggcggcga tcgtcgatgg tcggctggac ctcgagcgtc
    17701 tcgcggcggc cccggacgag gaggtcgtgt cggcgctcac cgccttgccg gggttcggcc
    17761 gctggagtgc ggagatctac ctgctcttcg cgctgcagcg gcaggacgtc ctgccggccg
    17821 acgacctggc cctgcagatt gccgcccagc gcctgaagaa cctcgaagtg cgaccgaacg
    17881 cccgggattt gcgggcactt gccgtggcct ggtcgccatg gcgcggcgcc gcctcggtct
    17941 ttctctggca ttactacggt gccgcgaccc tggacagcgt tgcatagcct tggtgccggt
    18001 gccgaacttg tggccagccg ggccgtctgc tagatcaccc tttacgcgaa tttcctcgcc
    18061 agcctgacct tcaggcccgc cgttccagcg ctcgggaagt acaccgcttg gccgcctcac
    18121 tgccccccaa gggattcgag atcgtcccca tcgaggagga gatgaagcgc tcctacctcg
    18181 actacgcgat gagcgtgatc gtcagccggg cgctgcccga cgttcgggac ggcctgaagc
    18241 ccgtccagcg ccgcatcctc tacgccatgc gcgtcgccgg ctacgatatc gggcgaccgc
    18301 accgcaagtc cgcgctcatc gtcggcgacg tgatgggcaa gtaccatccg catggcgacg
    18361 gcgccatcta tgacgccatg gtgcgcatgg cgcagcactg gtcgatgcgc ctcatgctga
    18421 tcgatggcca gggcaatttc ggctcgatgg acaacgaccc gccggcggcg atgcgttaca
    18481 ccgaggcgcg cctggcccgg ccggcggcag cactgctcga cgacatcgac aaggacacgg
    18541 tgggcttccg tcccaattac gacgagagca tcagcgagcc ggaggtcctg ccggcgcagt
    18601 acccgaacat tctggtcaat ggcgccggcg gcatcgcggt cgggatggcg accaacatcc
    18661 cgccgcacaa cctgggcgag atcatcgacg cgaccatcgc cctgatcgac gatcccgagc
    18721 tcgacatcgc cgacctcatg gagatcgtcc agggcccgga cttccccacc ggcggcatca
    18781 ttctcggccg ggccggcatc cagcaggcct acatgaccgg tcgcggcagc ctcaccgtcc
    18841 gcagccgttg cacgatcgaa gagatccgca aggaccgcca ggcgatcctc gtgcacgagg
    18901 ttccctatct ggtcaacaag gcgcggctgg tcgagcggat cgccgaggtg gtgcgcgaga
    18961 agcgggtcga gggcatctcc gatctgcgtg acgaatccga ccggcatggc gtgcgcgtgg
    19021 tcatcgagat caagcgcgac gccgatgccg aggtggtgct caaccagctc tacaagttca
    19081 cgccgctgca gaccggcttc ggcatcaaca tggtggcgct gaacaacggc cgcccgatgc
    19141 agatgacgct ggtcgaggtg ctgcaggcgt tcatcgcgtt ccgcgaggat gtgatcctca
    19201 ggcgtaccgc ctacgatctg gccaaggccc gcgagcgggc gcacatcctg gtcggcctcg
    19261 ccgtcgcggt cgccaatatc gacgaggtca tcgcgctgat ccgcgccgca cccgacccgc
    19321 agacggccaa ggccggcctg ctcgagcggg cctggccggc ggaggcgatc ctgccgctgc
    19381 tcgcgctcgt cgaggacggc acggcggcgg ccaccgagta ccgcctcagc gagcggcagg
    19441 cccaggccat cctcgacctg cggctgcagc ggctgaccgg gctcgagcgc gagaagatca
    19501 ccgccgagct ggccgagatc gccggggcga tcaaggagct gctcttgatc ctgcagtcgc
    19561 gcgagcggct gctcgaggtg atgaaggcgg agctgaccga tgtccgcagc cgtttcgccg
    19621 atgagcggcg caccttcatc gacctcgacg ccgagctcga catcgacatc gaggacctga
    19681 ttccgcgcga ggacatggtc gtcaccgtga cccacaaggg ctacatcaag cgggtgccgc
    19741 tttcgacctt ccgcgcccag cgccgtggcg gcaagggccg ggccggcatg cgcacgcacg
    19801 aggacgattt cgtcgcccgg ctgttcgtgg cgagcaccca tacgcccgtg ctcttcttct
    19861 cgagcctcgg gcgggtctac aagctcaagg tctacaagct gccattgggc aacccgcagg
    19921 cccgtggcaa ggcgatgatc aacctcttcc cgcatctcgt ggaaggcgaa tggatcaccg
    19981 ccatcctgcc tttgccggag gacgaggcga cctgggtgga gctttccgcc gtgttcgcga
    20041 cggctcgtgg caaggtgcgg cgcaacgacc tcgccgattt cacgcgggtg ccggtcaacg
    20101 gcaagatcgc catgggcctc gacgatgggg accggctgat cggtgtcgat gtctgcgacg
    20161 acagccacga cgtgctcctc gtgacgggcg gcggcaaggc gatccgcttc gccgtcgagt
    20221 cgctgcgcgt gttccgctcg cgcacatcgg aaggggtcag gggcatcgac ctcgccggcg
    20281 aggatgcggt gatctccatg tcgatcctcg accacatcac gctctcgacc gaggagcgcg
    20341 acgagctggt gcgaatcgcc aacgcccggc gccgggcggc ggccct
//
LOCUS       DAOTFI010000065        20354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3179_length_20354_cov_6.228780, whole genome shotgun sequence.
ACCESSION   DAOTFI010000065 DAOTFI010000000
VERSION     DAOTFI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 20354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20354
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3179_length_20354_cov_6.228780"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="P5558_08440"
     CDS             <1..541
                     /locus_tag="P5558_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24376.1"
                     /translation="ERTIKAFGGLEIVVNNAGVSHRNQPMLDVDEAEFDRVFAVNVKS
                     IFHMAHAVIPHFRGAGGGVILNVGSTAGLRPRPGLTWYNASKAAANLLSKSMAVELAP
                     DHIRVCALAPVIGETALLETFMGVPDTPENRARFVATIPLGRMSQPQDIANAALWLAS
                     DDASFITGVVLEVDGGRCV"
     gene            522..2156
                     /locus_tag="P5558_08445"
     CDS             522..2156
                     /locus_tag="P5558_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HRY24377.1"
                     /translation="MAGAASDRAWQPLFQTGPDQTGYRRLDLPPPGLHELGGRRFLEV
                     TPETLQGLAEAAFFEVQHLFRSAHLEGLRRVVLDPESSPADRFVSLELLKNAVIAAGR
                     VFPSCQDTGTAIVIGRKGQAVLTTGDDREPLSAGIRSAYARHNLRFSQLAPLTMSEEV
                     NTGTNLPAQIGIEAVPGEVFEFLMIAKGGGSANKTFLFQETRRLLEPERLLAWLEAKV
                     GEIGTTACPPYHLAIVIGGLSAEQCLKTVKLASTRELDGLPETGDAFGRAFRDRGLEE
                     RVLDMTRGIGIGAQFGGRHFCHDVRIVRLPRHNGSLPVGIGVSCSADRQIRARITADG
                     VFLEQLEEDPARFLPDAQIDIGEATPLDLDRPMAAIRADLARLEVGAPVLLSGTLVVA
                     RDLVHAALAGRLARGEALPGWIQDHPVYYAGPAKTPEGHASGSFGPTTAARMDAYMAG
                     FQAAGGALVTLAKGNRSAEVAASCKAHGGFYLATIGGVAARLGRDMIKAVEVIDFAEF
                     GMEAVWRIEVVDVPAFLVIDDKGNDFYRRVRRRSAA"
     gene            2191..3012
                     /locus_tag="P5558_08450"
     CDS             2191..3012
                     /locus_tag="P5558_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY24378.1"
                     /translation="MTDPVATHYARGDDLASAIGRRLTEAGKDLARLTTADLAPVDEF
                     HIRGRDATLELSRGMGLTPESHVLDLGSGVGGPARTVAEAHGCRVTGIDLTPAFCDAA
                     GVLTDWVGLADRVRFEVGDVTALPFADASFDAAMTLHVAMNIKAKDRLYAEARRVLRP
                     GGVLAIYDVLQGEGGAVVYPVPWAADAAISHLATPDEMTALLEGAGFTIVEREDSTGR
                     GEVFLGAVAERLAAQGAPPVSLHALLGDDFTAMAANLARNLAERRVRALRYLCRL"
     gene            3086..3856
                     /locus_tag="P5558_08455"
     CDS             3086..3856
                     /locus_tag="P5558_08455"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRY24379.1"
                     /translation="MSDHTNRFSVAGKRALVTGATKGIGLEICRVLASAGADIAAVGR
                     DAAGLEEVRLAVESAERRCVTIRADLASAAETERAAAEALEAFGTIDILVNNAGIAVV
                     GPALTTSVEDWDLVMAVNLRAPFIMAKALAPKMIEQGSGKIINISSQTGVVGLDDHSA
                     YSASKGGLNSLTMTLTSEWAKHNIQVNAVCPTVILTPMGEQVWGDPAKGDPMKAKIPA
                     ARFGKPIEVADLVLFLASPASDLINGQAILIDGGFTAI"
     gene            complement(3866..5245)
                     /locus_tag="P5558_08460"
     CDS             complement(3866..5245)
                     /locus_tag="P5558_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRY24380.1"
                     /translation="MRERPRDHRQAPSGFRRLAGLLPAVVLLVASHAGMAQQAADAPD
                     PGPPIAVGAEAISDADIAARLRGILGALDSYGDVRATVESGVVTLEGSVAEANEVAPL
                     GRLVGRIDGVVTVANRVEASGDVAERLNPVVTRLETRLRGLVGALPLLLVALLVLGAF
                     VGLGALAARPQRLYGRISPNAFIADIYRQLVRIAFIFAGVVAALEIMGATALLGTLLG
                     AAGIIGLAVGFGVRDTIENFVASLMLSVRQPFEPNDLVELEGEVGHVIRLTSRATVLL
                     SPDGNHIRLPNSVVFKSKIVNYSRNPDRRFSFDLGVDPAEDLALALRTGIDAVRTLPF
                     VLDDPPAAAWVEEVGESTVALRFTGWIDQRRTEFSMARGEAIRMAKNALETGGFTLPE
                     PLYRLRLESGGQFPPAPAAEAPRRPAPASVAEVMDPAKAGVIDQKVEAERRSAGGAED
                     LLSPSAPLE"
     gene            5421..6179
                     /locus_tag="P5558_08465"
     CDS             5421..6179
                     /locus_tag="P5558_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRY24381.1"
                     /translation="MAARLPFKIEICVENTDGLIAAQNAGADRAELCASLMEGGLTPS
                     QGMIECALRVATIPFHVIVRPRGGDFLYSAIELEAMRADVRHLRQLGVAGVVIGCLTP
                     DGAIDEARMKTLVEDAGPLAVTCHRAFDMAADPIEAIEALVRAGVHRVLTSGQRETAE
                     EGIPTLKRIMAAAAGRIGIMACGELNAANIARVRQAVDPDEMHFAAPRKEPSAMTFRN
                     TRIGMGGTDFDREYTNTVTDEALVRAMIRAARGG"
     gene            6311..6433
                     /locus_tag="P5558_08470"
                     /pseudo
     CDS             6311..6433
                     /locus_tag="P5558_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612205.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6510..8552)
                     /locus_tag="P5558_08475"
     CDS             complement(6510..8552)
                     /locus_tag="P5558_08475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRY24382.1"
                     /translation="MTDATPLLDTWTAPFGLPPYGRFEPVDFRAAFEAALAGHRAELE
                     AIATDPATPDFANTVAALERSGRNLRRVSALFFNLCGTDTDATLQAIERELAPVLARH
                     WNDLHQDPRLFARLDALRKAADPTLGTEEARVLERYTTAFVRSGAHLDEAARKRLAAI
                     AERLATLGTSFAQNLLADEQAYTLVLDGEADLAGLPDFVRAAAASAAAARGLEGKHVV
                     TLARSSIEPFLVFSTRRDLRETAFRAWLARGEKGGETDNRAIIKETIALRAERARLLG
                     FATFAHYRLADSMAKTPEAALDLLHTVWPRARRKAEADAAALQALIAAEGGNFDLAAW
                     DWRHYAEKRRQAELAIPPGAVEPYLQLDRLIEAAFSVAKRLFGITFEEQHGLELYHPD
                     VRAWDVRDRDGRHLGLFLGDYFARPAKRSGAWMSGLRGQEKLAGEIRPIIVNVMNFSK
                     PAPGAPALLGFDDARTLFHEFGHALHGLLSDVTYPLLAGTHVGRDFVEFPSQLLEHWL
                     EEPAILAEFARHHATGEPMPEDLAGRLAAARHFDQGFATLEYAACAILDLELHLLAEA
                     EGLDIGAFERERLAALGMPAAIAMRHRPPQFAHVFSGGGYASGYYSYLWSEVLDADGF
                     DAFREAGDPFDPSVAQRLREFVLAAGNRMDPVEAYRAFRGRDPEAQALLVKRGLAA"
     gene            8674..10158
                     /locus_tag="P5558_08480"
     CDS             8674..10158
                     /locus_tag="P5558_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY24383.1"
                     /translation="MLRRLRSILSWLLIRGLLILVLVLAVFLGTRIWEAETGAPLQAW
                     HKFVPEELDAEAIDRADWETWLAAEDRVMAEVRTQVTDKLEPSDRIAANRYFEGSPVY
                     PPSLARDWNRSYVLLPDGPPRGAAVFLHGLTDSPYSLRHIARRYRELGWATVAIRLPG
                     HGTVPGGLYDVEWPDWSAAVRLAVREAIHLAGEAVPLHIVGFSNGGALALKYTLEALD
                     DPALARPDRLILVSPMIGVTEFARFAGLAGLPAFFPRFARAAWIAVLPEFNPFKYNSF
                     PVNGARQSHLVTMALQAELARHERNGGLEALPPILAFQSVIDFTVSTPAVIRTLYDRL
                     PANGSELVLFDLNRSARFGPLLRPAAATAIDRLVPAPPRRYGLTAVVDPAPLGDGMVA
                     VTVPAGTDEAEEQPLGIAYPTDVFSLSHVALPFPLDDPLYGLEPAGNEDYGVNLGATA
                     VRGERGALIVSIDSVSRMSSNPFFPYILERIEAAIATPAPLAVR"
     gene            10166..11083
                     /locus_tag="P5558_08485"
     CDS             10166..11083
                     /locus_tag="P5558_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRY24384.1"
                     /translation="MIQLYRAVWRVSGRQQVLLIVLSLATAALAAAPLKLQQLVINLL
                     VEGGTVERLVWLCGAFLGAVLLSAGLKFLLNLRISVLGERVILMARERLYGNAVHDTR
                     AGTTEPIRRGTLVNMLTAEAEVVGGFAGSAIAGPLLQLGTLVSVLTFVFVQQPRLGLI
                     VAAVVIPQAVIVVALQKRINREVRARVTALRDASDRISQSDLLETDEAVAEDFRLTFT
                     IRRRIYAFKLSIKLALSVITALGAVAILFIGGWLVLQGRSDVGTVVASLSGLARIDGP
                     WRTLIQFFRQASTVRVNYEMLTRALAPRG"
     gene            11172..11621
                     /locus_tag="P5558_08490"
     CDS             11172..11621
                     /locus_tag="P5558_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRY24385.1"
                     /translation="MQQISKTRRALTGGAVPALALGLLLATGPAALAQQSGLSSALQQ
                     ERMAFEVADTDGDMRISPGEFARDAAAGFSSLDTDGSLTLTPAELGEHDPALFGPIDG
                     DGDGALTFDEVMTYKMKAFEAADTDGDGYLSFDEMVGAAEDQLGAAQ"
     gene            11630..12040
                     /locus_tag="P5558_08495"
     CDS             11630..12040
                     /locus_tag="P5558_08495"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRY24386.1"
                     /translation="MSHRRTPVAMAALAGILALSLAACEGGGGGTGSAGVGTVGGAAV
                     GAGAGRLLFGNSTTGMLIGAGVGGLAGNMTLDRQAEERRAQEQQAAQDAQMRRQLDFE
                     RQQALQAEETRRQIEEQRLFEEWRRTQNTSAPAG"
     gene            complement(12156..12413)
                     /locus_tag="P5558_08500"
                     /pseudo
     CDS             complement(12156..12413)
                     /locus_tag="P5558_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase domain-containing
                     protein"
     gene            complement(12513..12728)
                     /locus_tag="P5558_08505"
     CDS             complement(12513..12728)
                     /locus_tag="P5558_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRY24387.1"
                     /translation="MSLALYGHPFSSYTQKVLIAIYENGTPFEFRCIGPDRPAHVAAW
                     RRRWPLRKFPLLVDGERQVVETSIIVE"
     gene            12865..13122
                     /locus_tag="P5558_08510"
     CDS             12865..13122
                     /locus_tag="P5558_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRY24388.1"
                     /translation="MSTRIDLPPELEAFARDCVTSGRYSDTSDVIRAALRRLQDAEAR
                     RSSFNAMLAETRAEAERDGTFDLDTVLAEADAIIESRSGAQ"
     gene            13126..13428
                     /locus_tag="P5558_08515"
     CDS             13126..13428
                     /locus_tag="P5558_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY24389.1"
                     /translation="MGKAQLSPRARRDLLAAVAWIARDNPPAARALRDAVARASERLS
                     LLPRTGTERPDIADPPVRFLVLTGFPYLLVYDAESDPPLILRIIHGARDLPGTLGE"
     gene            complement(13482..13631)
                     /locus_tag="P5558_08520"
     CDS             complement(13482..13631)
                     /locus_tag="P5558_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY24390.1"
                     /translation="MVLDSSAILAILLDEPERAGFTRRIEQDQTRLVSAATLVKVTIV
                     MGQRP"
     gene            complement(13631..13888)
                     /locus_tag="P5558_08525"
     CDS             complement(13631..13888)
                     /locus_tag="P5558_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRY24391.1"
                     /translation="MALSLKDAETDRLAREVARLTGESLTEAVRRALAERLARERRKR
                     GEAVGLAGRLDALAREGADLPDLDRRSAEEILGYDACGLPG"
     gene            14576..15244
                     /locus_tag="P5558_08530"
     CDS             14576..15244
                     /locus_tag="P5558_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24392.1"
                     /translation="MRLIITDVTEMHGGNYCVAGWDPQARRMVRPLPSGANWTAGLLQ
                     QHRVVPGVSIDVTPTGQHHQNALPHRTEDLPVDRATIQHVNSGPINWFAAGAPLTHAT
                     VSDAFSGHIAHNSIWNNVRQGVYVKVGTQTGSLAAVRLGRNAVQFLEEFNKLRAKLND
                     GGTNYKLPVSSLALKSAWQQGGLVAVQQALPTTQQFHVRLGLARAFGNPPEKCYLMVN
                     GIHG"
     gene            15237..15866
                     /locus_tag="P5558_08535"
     CDS             15237..15866
                     /locus_tag="P5558_08535"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRY24393.1"
                     /translation="MVSDMSQPTIFSIGHSTHNYEYFLELLRNAGVTAIADVRTSPYS
                     RHFPQFSRVLLKDKLQVDGIAYSFLGKELGGRPTGDKYYCNGVADYERMAQSPDFARG
                     LQRVLDGARKYNIALMCSEHEPLDCHRCLLVGRALTACEVDLKHILTDGTIEDHRKIE
                     ERLLKSEGKHGDDLFLSPVERLAEAYRKRARKVAYAEQPDGSATHSTME"
     gene            15870..16316
                     /locus_tag="P5558_08540"
     CDS             15870..16316
                     /locus_tag="P5558_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRY24394.1"
                     /translation="MTRFKVNTIGFTKSTAKNFFDRLLKSGVRKVVDVRLHNTSQLSG
                     FAKADDLAYFLMKIGGIEYVHQPLLAPTDPMLKAYKKEKGDWGTYEERFLGLMAEREV
                     QKRFKPEMFDNACLLCSEDKPHYCHRRLVCEYLKDEWNGVLDVHHL"
     gene            17310..>17905
                     /locus_tag="P5558_08545"
     CDS             17310..>17905
                     /locus_tag="P5558_08545"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRY24395.1"
                     /translation="MAPGRGENPLPDPPRGVTFDPQGRGIDGGMMRAVTTYVRWVDAL
                     NRVVGRLTMLLIFVMMGVLLYSSISRSAFNLPLIWVVEMSQFMLVAYFLLGGGYSMQQ
                     GAHVRMDLFYAKWAPRKRAFTDTITAFCLIFYLVVLMMGGLSSTLYALEYGQKNYSSW
                     APPLAPIKIIMCIGIALMLLQAVAFLVRDIARLRGIELP"
     assembly_gap    17906..18005
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18006..19145
                     /locus_tag="P5558_08550"
     CDS             <18006..19145
                     /locus_tag="P5558_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY24396.1"
                     /translation="LTLPLFVYMGYVLAESGIANDLYDMLHVWSGGLKGGLAIGTVVL
                     MVIVSAMNGLSVAGLAIGATIALPEMLRRGYDKIMVTGVIQAGSSLGILVPPSVVLIL
                     YGMIARQPVTQLWLAGVLPGLMMAGFFILYIAVRCRLDPSLAPPLPAAERDIPLRQKL
                     GLLRAGILPLLIFFSMTGLFVAGFTSLVESAAYGAAAATVAAAVKRRLTIKVLEETVR
                     KTLAISCMFLWIIMAALCFGAVFDGLGAGRALRGLFLDEWHLSPWTVLIMMQLSFIIL
                     GMFLDDTAMLVIVAPLYVPLAIALDFDPIWYGVLYTVTCQIAYITPPFGYNLFLMRAM
                     APPEISLGDIYRSIVPFVGIMGLTLVILMLFPEIALWLPSWYAGR"
     gene            19195..20268
                     /locus_tag="P5558_08555"
     CDS             19195..20268
                     /locus_tag="P5558_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY24397.1"
                     /translation="MTIKNPRRRDFLGKAGATTLGAGAVAAASTFPAPYVKAQGPIRW
                     RLQTYAGPALAEHVIKPSIDQFNKVANGEMEIELYFADQLVPTPELFRAVQQGTIDAV
                     QSDDDSIGAPVDVSVFGGYFPFASRYSLDVPTLFNHWGLKEIWQEAYGEIDGVTWLSA
                     GSWDPCNFVTRDPIRSVADLEGKRVFTFPTAGRFLTQFGVVPVTLPWEDIEVALQTGE
                     LDGVAWCGITEAYTVGWADVTSYYLTNNISGAWVGSYFANTERWNELPEHLKGLFTLA
                     MDSSHYYRQHWYWWGEAYYRTTGDKLELTTIPEEEWSAIEGAAEVFYEEIAAESERSA
                     RIIQIIKNYRAAMHKAGPPYRFS"
BASE COUNT         3262 a         7038 c         6731 g         3223 t
ORIGIN      
        1 cgagcggacg atcaaggctt tcggcggcct cgagatcgtc gtcaacaacg cgggcgtgag
       61 ccaccggaac cagccgatgc tcgacgtcga cgaggccgag ttcgaccggg tcttcgccgt
      121 caacgtcaag agcatcttcc acatggcgca tgccgtgatt ccgcatttcc gcggggcggg
      181 cggcggggtg atcctcaatg tcggctcgac cgccgggctc aggccgcggc cgggcctcac
      241 ctggtacaat gcctcgaagg cggcggcgaa cctgctcagc aagtcgatgg cggtggagct
      301 cgcacccgac cacatccggg tctgcgcgct cgcgccggtg atcggcgaga ccgccctgct
      361 cgagaccttc atgggcgtgc ccgacacgcc ggagaaccgc gccaggttcg tcgccaccat
      421 cccgctcggc cggatgagcc agccgcagga catcgccaac gccgccctct ggctcgcctc
      481 ggacgatgcg agcttcatca cgggcgtggt gctcgaggtc gatggcgggc gctgcgtctg
      541 atcgggcctg gcagccactc ttccagaccg ggccggacca gaccgggtat cgccggctcg
      601 acctgccgcc gcccgggctg cacgagctgg gcggcaggcg ctttctcgag gtaacgccgg
      661 aaacacttca gggcctcgcc gaagccgcct tcttcgaggt gcagcatctc ttcaggagcg
      721 ctcatctcga gggccttcgg cgggtcgtcc tggacccgga aagctcgccg gccgaccggt
      781 tcgtcagcct cgagcttttg aagaacgcgg tgatcgccgc gggccgcgtc tttccctcct
      841 gtcaggacac cggcacggcc atcgtcatcg gcaggaaggg ccaggccgtg ctgaccacgg
      901 gcgacgaccg cgagccgctc tcggccggca tccggagcgc ctatgcccgc cacaacctgc
      961 gcttcagcca gctcgcgccg ctcacgatga gcgaggaggt gaacaccggc accaacctgc
     1021 cggcgcagat cgggatcgag gcggtgccgg gcgaggtgtt cgagttcctg atgatcgcca
     1081 agggcggcgg gtcggcgaac aagacgttcc tcttccagga gacgcggcgg ctgctggagc
     1141 cggagcggct gctcgcctgg ctcgaggcga aggtgggcga gatcggcacc accgcctgcc
     1201 cgccctacca cctcgccatc gtcatcggcg ggctctcggc ggagcagtgc ctgaagacgg
     1261 tcaagctcgc ctcgacccgc gagctggacg gcctgcccga gacgggcgat gccttcgggc
     1321 gggcgttccg cgaccgcggg ctggaagagc gggtgctcga catgacgcgc ggcatcggca
     1381 tcggcgcgca gttcggtggc cggcatttct gtcacgacgt gcggatcgtc cgcctgcccc
     1441 ggcacaatgg ctcgctgccg gtcggcatcg gcgtctcctg ctcggccgac cggcagatca
     1501 gggcgcggat caccgccgac ggggtctttc tcgagcagct cgaggaggac ccggcgcgtt
     1561 tcctcccgga cgcccagatc gacatcggcg aggcgacgcc ccttgacctc gaccggccga
     1621 tggcggcgat ccgcgccgat ctggcgaggc tcgaggtcgg ggcgccggtg ctgctttcgg
     1681 gcacgctggt ggtcgcccgc gacctggtcc acgcggcgct cgccgggcgg ctggcgcggg
     1741 gcgaggcgct gccggggtgg attcaggatc acccggtcta ctatgccggc ccggcaaaga
     1801 cgccggaagg acatgccagc ggcagcttcg ggccgaccac ggcggcgcgc atggacgcct
     1861 acatggcggg cttccaggcg gccgggggtg ccctggtgac gctcgccaag ggcaaccgct
     1921 cggccgaggt ggcggcgagc tgcaaggcgc atggcgggtt ctatctcgcc accatcggcg
     1981 gggtggcggc ccggctcggc cgggacatga tcaaggcggt cgaggtcatc gacttcgccg
     2041 aattcggcat ggaggcggtc tggcgcatcg aggtcgtgga cgtgccggcg ttcctcgtga
     2101 tcgacgacaa gggcaacgac ttctatcgcc gggtgcggcg ccggtctgcg gcctgagcac
     2161 ccggctccct cgaagggacg ggaggtctgc gtgaccgatc cagtggcgac ccattatgcc
     2221 cgcggcgacg acctcgcctc tgccatcggc cggcggctga ccgaggcggg gaaggacctc
     2281 gcgaggctca ccacggccga cctggcgccg gtcgacgagt tccacatccg cggccgcgac
     2341 gcgacgctgg agctgtcccg gggcatgggc ctgacgcccg agagccatgt gctcgacctc
     2401 ggcagcggcg tcggcgggcc ggcgcgcacc gttgccgagg cgcatggctg ccgcgtcacc
     2461 ggcatcgacc tgaccccggc cttctgcgac gccgccggag tgctcaccga ctgggtcggc
     2521 ctcgccgacc gcgtgcggtt tgaggtgggc gacgtcaccg ccctgccctt cgccgatgcc
     2581 agcttcgatg ccgccatgac cctgcacgtg gcgatgaaca tcaaggccaa ggaccggctc
     2641 tatgcggaag cccggcgcgt gctgcggccg ggcggcgtgc tggcgatcta cgacgtgctg
     2701 cagggcgagg gcggcgctgt cgtctatccc gtcccctggg cagccgacgc cgcgatcagc
     2761 cacctcgcca cgccggacga gatgaccgcc ctgctcgagg gtgcgggctt caccatcgtc
     2821 gagcgcgagg attcgaccgg gcggggggag gtcttccttg gggccgtggc ggagcggctc
     2881 gcggcccaag gtgcgccacc ggtcagcctc cacgctttgc tcggcgacga cttcacggcg
     2941 atggcggcca atctggctcg caacctcgcc gagcggcggg tgcgcgccct tcgctatctc
     3001 tgccggctct gagcctgccc tgttcgaatc ggcaccgatc ggctagcacg acccgagcag
     3061 tcgggtgaag caagggagag cggcgatgag cgaccatacc aaccggttca gcgtggccgg
     3121 caagcgggcg ctggtgaccg gggcgaccaa ggggatcggg ctggagatct gccgggtgct
     3181 ggcatccgcc ggcgccgaca tcgccgctgt cgggcgggat gccgccgggc ttgaggaggt
     3241 ccgactggcg gtcgaatcgg cggagcggcg ctgtgtcacg atccgggccg acctcgcctc
     3301 ggcggcggaa accgagcggg ccgcggccga ggcgctcgaa gccttcggga cgatcgacat
     3361 cctggtcaac aatgccggca tcgccgtggt cggcccagcg ctcaccacct cggtcgagga
     3421 ctgggacctg gtgatggcgg tcaatctccg cgcccccttc atcatggcga aggcgctggc
     3481 cccgaagatg atcgagcagg gttcgggcaa gatcatcaac atcagcagcc agacgggtgt
     3541 cgtcgggctc gacgaccact cggcctattc ggcgtccaag ggcgggctca actcgctgac
     3601 catgacgctg acctccgagt gggcgaagca caacatccag gtcaacgccg tctgcccgac
     3661 cgtcatcctg acaccgatgg gcgagcaggt ctggggcgat ccggccaagg gcgaccccat
     3721 gaaggcgaag atcccggccg cccgcttcgg caagccgatc gaggtggccg acctcgtcct
     3781 tttcctcgcc agcccggcct cggacctgat caacggccag gcgatcctga tcgacggcgg
     3841 cttcaccgcg atctgaggcg cgtcgctatt ccagcggggc cgagggcgag agcagatcct
     3901 cggccccgcc ggcagagcga cgctccgcct cgaccttctg gtcgatcacc ccggccttcg
     3961 ccgggtccat gacctcggcc acgctcgccg gtgcggggcg cctgggggcc tccgccgccg
     4021 gggccggcgg gaactgccca ccgctctcca agcgcagccg gtagagcggc tccggcaggg
     4081 tgaagccgcc ggtttcgagg gcgttcttgg ccatccttat ggcctccccc ctcgccatgg
     4141 agaattccgt ccggcgctgg tcgatccagc cggtgaagcg cagcgccacg gtggattccc
     4201 cgacctcctc gacccaggcg gcggcaggcg gatcgtcgag cacgaagggc agcgtgcgca
     4261 ccgcgtcgat cccggtcctc agggcgaggg ccaggtcctc ggccggatcg acgccgagat
     4321 cgaagctgaa gcgccggtcg gggttgcggc tgtagttgac gatcttgctc ttgaagacga
     4381 ccgagttggg cagccggata tggttgccgt cgggcgagag cagcacggtg gcgcgcgagg
     4441 tgaggcggat gacgtggccg acctcgccct ccagctcgac caggtcgttc ggctcgaacg
     4501 gctggcgcac gctgagcatc aggctggcga cgaagttctc gatggtgtcg cgcacgccga
     4561 agccgacggc gaggccgatg atgccggcag cgccgagcag cgtgccgagc aaggccgtgg
     4621 cgcccatgat ctcgagggcg gcgaccacgc cggcgaagat gaaggcgatg cgcacgagct
     4681 ggcggtagat gtcggcgatg aaggcattgg gggagatgcg gccgtagagc cgctgcggcc
     4741 ttgcggccag ggccccgagg ccgacgaagg cgccgagcac gagaagcgcc acgagcagca
     4801 gcggcaaggc gccgacgagg ccgcgcaagc gggtttcgag ccgggtgacg accgggttca
     4861 ggcgttcggc gacatcgccc gaggcctcga cgcgattggc gaccgtcacc acgccgtcga
     4921 tgcgaccgac gagccggccc agcggcgcga cctcgttggc ctcggcgacg gagccttcga
     4981 gcgtcaccac acccgattcg accgtggcgc gcacgtcgcc atagctgtcc agggcaccga
     5041 ggatgccgcg caggcgggcg gcgatgtcgg cgtcgctgat cgcttcggca ccgacggcga
     5101 tgggtggccc cgggtcgggc gcatcggctg cctgctgggc catgccggca tggctggcca
     5161 ccaacaggac cacggcgggc aggagcccgg cgagccggcg aaagccggac ggcgcctgcc
     5221 ggtggtctcg tggtcgctcg cgcatggcct gcggtctccg gtagcctcgc tcggcagact
     5281 gcgccagcga cggcggtcgg cgcaatgccg agaggaaccg atcccggcca ttccggtttc
     5341 gtcgcggtcg gctacaaggg ggtggtcccc tgcccgttcc tgacggccgc gcgccggccg
     5401 attccccgga gaaccgcccg atggccgcac gcctgccctt caagatcgag atctgcgtcg
     5461 agaacaccga cggcctgatc gccgcccaga atgccggcgc cgaccgggcc gagctctgcg
     5521 cctccctcat ggaagggggg ctgacgccga gccagggcat gatcgagtgc gcgctgcggg
     5581 tcgccaccat cccgttccac gtcatcgtcc gcccgcgcgg cggcgatttc ctctactcgg
     5641 cgatcgagct cgaggcgatg cgggccgacg tccgccatct ccgccagctc ggcgtcgccg
     5701 gggtggtgat cggctgcctg acgccggatg gggccatcga cgaagcgcgc atgaagaccc
     5761 tcgtcgagga cgccggcccg ctcgccgtca cctgccaccg cgccttcgac atggcggccg
     5821 accccatcga ggcgatcgag gcgctggtcc gcgccggcgt ccaccgggtg ctgaccagcg
     5881 gccagcgcga aacggcggag gaaggcatcc cgacgctcaa gcggatcatg gccgccgccg
     5941 ccggccgcat cggcatcatg gcctgcggcg agctgaatgc cgcgaacatc gcccgcgtgc
     6001 gccaggccgt cgacccggac gagatgcact tcgccgcccc ccgcaaggag ccgagtgcga
     6061 tgaccttccg caatacgcgg atcggcatgg gcggcacgga tttcgaccgg gagtacacga
     6121 acacggtgac cgacgaggcg ctggtgcggg cgatgatcag ggcggcgcgg ggtgggtgag
     6181 ctatgagatg gtagctgcac cgaacgtgac gggggtttct cgcagttcta ggagcctgtc
     6241 cgaacagttg ctgagtggaa tcgattgctc atgacgtcct tgttctgcca ggagcaagga
     6301 ggacatggag atctggaccg cttccgctcg ccgtcagtat cagcggttgg ggcgctgcta
     6361 cgcaagcgat ctgacggacg aggagttcgc ggtgatcgag ccgatgctgc cgccggcgaa
     6421 tccgggcggg cgaggcacga acatatcatg ccatatgaag cagcgtggcc gcttctaggt
     6481 tcggggcgat acggtcggcg tcggtgcctc tacgccgcca gcccccgctt caccagcagc
     6541 gcctgcgcct ccggatcacg cccacgaaac gcccgatagg cctctaccgg gtccatccgg
     6601 ttgccggcgg cgagcacgaa ctcccggagc cgctgcgcca ctgacgggtc gaaagggtcg
     6661 ccggcctcgc ggaaggcgtc gaagccgtcg gcgtcgagga cctcggacca gagatagctg
     6721 tagtagcccg aggcgtagcc gccgccggag aagacgtggg cgaactgggg cgggcggtgg
     6781 cgcatggcga tggcggcggg catgcccagc gcggcgaggc gctcccgctc gaaggcgccg
     6841 atgtcgagcc cttccgcctc cgccagcagg tgcagctcga ggtcgagaat ggcgcaggcg
     6901 gcgtattcga gcgtggcgaa gccctggtcg aaatggcgcg cggcggcgag gcgccctgcc
     6961 aggtcctcgg gcatgggctc gccggtcgcg tgatggcggg cgaactcggc caggatcgcc
     7021 ggctcctcca gccagtgctc gaggagctgc gacgggaact cgacgaagtc gcggccgaca
     7081 tgcgtgccgg cgaggagcgg gtaggtgacg tccgagagga ggccgtgcag cgcgtggccg
     7141 aactcgtgga agagcgtgcg cgcgtcgtcg aagccgagca gcgccggcgc acccggggcc
     7201 ggcttcgaga agttcatgac gttgacgatg atcgggcgga tctcgccggc cagcttctcc
     7261 tgcccgcgca gcccgctcat ccaggcgccg ctccgcttcg ccggccgcgc gaaatagtcg
     7321 ccgaggaaga gcccgagatg ccggccgtcc cggtcgcgca cgtcccaggc gcggacgtcc
     7381 gggtggtaga gctcgagccc gtgctgctcc tcgaacgtga tgccgaagag gcgcttcgcc
     7441 acgctgaagg ccgcctcgat cagccggtcg agctggaggt agggctcgac ggcaccgggc
     7501 gggatggcga gctcggcctg ccggcgcttc tcggcatagt ggcgccagtc ccaggcggcg
     7561 agatcgaaat tgccgccctc ggcggcgatc agcgcctgca gcgccgccgc gtccgcttcc
     7621 gccttgcgcc gcgcccgggg ccagacggtg tgcagcaggt cgagggccgc ctccggcgtc
     7681 ttcgccatgc tgtcggcgag gcggtagtgg gcgaaggtgg caaagcccag gagccgggcc
     7741 cgctcggccc ggagtgctat ggtttccttg atgatcgccc ggttgtcggt ctcgccgccc
     7801 ttctcgcccc gggcgagcca ggcccgaaag gccgtctcgc gcaagtccct gcgcgtcgag
     7861 aacacgagga agggctcgat gctcgagcgt gccagggtga cgacgtgctt gccctcgagc
     7921 ccgcgcgccg cggcggcgct ggcggcggcg gcgcggacga agtccggcag gcccgccagg
     7981 tccgcctcgc cgtcgaggac gagcgtgtag gcctgctcgt cggcgagcag gttctgggcg
     8041 aagctggtgc cgagcgtggc cagccgctcg gcgatggcgg cgagccgctt tctcgccgcc
     8101 tcgtccagat gagcaccgct gcgcacgaag gcggtcgtgt agcgttccag cacgcgcgcc
     8161 tcctcggttc cgagcgtcgg gtcggcggcc ttccgcaggg cgtcgaggcg ggcgaagagg
     8221 cgggggtcct ggtggaggtc gttccagtgg cgggcgagca ccggcgccag ctcgcgctcg
     8281 atcgcctgga gcgtggcatc ggtgtcggtg ccgcagagat tgaagaaaag ggccgaaacc
     8341 cgccgcagat tacgcccgct ccgctcgagg gcggcgacgg tgttggcgaa gtcgggtgtc
     8401 gccggatcgg tggcgatcgc ctcgagctcg gcccggtgac cggccagtgc tgcctcgaag
     8461 gcggcgcgga aatccaccgg ctcgaagcgc ccatagggcg gcaggccgaa gggtgctgtc
     8521 caggtgtcga gcagcggcgt ggcgtcggtc atgggtcggg ttcccgaggg caatggcggc
     8581 agcgggcata gcgcggccgg ggccgcctgt cagccacgct gcccggccca cccggccatt
     8641 gccaacggcc ggtgccacgc tatgcaggga gccatgctgc gccgcctccg gtcgatcctc
     8701 tcctggctcc tgattcgcgg gctcctgatc ctcgtgctcg tgctcgcggt ctttctcggc
     8761 acgcggatct gggaggccga gaccggggca ccgctccagg cctggcacaa attcgtgccc
     8821 gaggagctcg atgccgaggc catcgacagg gcggactggg agacctggct cgccgccgag
     8881 gaccgggtca tggccgaggt tcgaacgcag gtcacggaca agctcgagcc ttcggaccgg
     8941 atcgccgcca atcgctactt cgagggcagc ccggtctatc cgccgagcct cgcccgggac
     9001 tggaaccgct cctacgtgct cctgcccgat ggcccgccca ggggcgccgc cgtcttcctc
     9061 cacgggctca ccgattcgcc ctacagcctg cgccacatcg ccaggcgcta ccgggagctc
     9121 ggctgggcca ccgtcgccat ccgcctgccg ggccacggca cggtgccggg cgggctctac
     9181 gacgtcgagt ggccggactg gtcggcggcg gtgcggctcg cggtgcgcga ggccatccat
     9241 ctggccggcg aggcggtgcc gctgcatatc gtgggcttct ccaatggcgg ggcgctggcg
     9301 ctcaaatata cgctggaagc gctcgacgat cccgccctcg cccggcccga ccggctgatc
     9361 ctggtctcgc ccatgatcgg cgtcaccgag ttcgcccgct tcgccgggct cgccgggctg
     9421 ccggccttct tcccgcgctt cgcccgggcc gcctggatcg ccgtcctgcc cgagttcaac
     9481 cccttcaagt acaactcctt cccggtcaac ggggcgcgcc agtcgcatct cgtcaccatg
     9541 gccctgcagg ccgagctcgc ccgccatgaa cgcaacggcg gcctggaggc gctgccgccg
     9601 atcctcgcct tccagtcggt gatcgacttc accgtcagca cgccggccgt gatccgcacc
     9661 ctctacgacc ggctgccggc gaacggcagc gagctcgtgc tcttcgacct caaccgcagc
     9721 gcccgcttcg gcccgctgct ccggccggcc gcggccacgg cgatcgaccg gctcgtgccg
     9781 gcgccgccgc gccgctacgg cctgaccgcg gtggtcgacc ccgccccgct cggcgacggc
     9841 atggtggcgg tcaccgtgcc ggccggcacc gacgaggcgg aggagcagcc gctcggcatc
     9901 gcctacccga ccgacgtgtt ctcgctctcg cacgtcgccc tgccctttcc cctggacgac
     9961 ccgctctacg ggctggagcc ggcgggcaac gaggattacg gcgtcaatct cggcgccacg
    10021 gcggtgcgcg gcgagcgcgg tgctttgatc gtcagcatcg attcggtctc gcgcatgtcg
    10081 tccaacccct tcttccccta catcctcgag cggatcgagg cggcgatcgc gacccccgcc
    10141 ccgctggccg tccgatgagg cgtcgatgat ccagctctac cgtgccgtct ggcgcgtcag
    10201 cggccgccag caggtcctgc tgatcgtcct ctccctggcg accgcggccc tggccgcggc
    10261 accgctcaag ctccagcagc tcgtcatcaa cctgctggtc gagggcggca cggtcgagcg
    10321 gctcgtctgg ctctgcggcg ccttcctggg cgccgtgctg ctcagcgccg ggctcaagtt
    10381 cctgctcaac ctgcgcatct ccgtgctggg cgagcgcgtc atcctgatgg cgcgcgagcg
    10441 gctctacggc aatgcggtgc acgacacccg agccggcacg acggagccga tcaggcgcgg
    10501 cacgctggtc aacatgctga ccgccgaggc ggaggtcgtc ggcggcttcg cgggctcggc
    10561 gatcgcgggc ccgctcctgc agctcggcac gctggtcagc gtgctcacct tcgtcttcgt
    10621 ccagcagccg cggctcggcc tgatcgtggc cgccgtggtg atcccgcagg cggtgatcgt
    10681 ggtggcgctg cagaagcgca tcaaccgcga ggtgcgcgcc cgggtgaccg ccctcaggga
    10741 cgcctcggac cggatctcgc agagcgatct tctggagacg gacgaggcgg tggccgagga
    10801 cttccggctg accttcacga tccgccggcg gatctacgcc ttcaagctct cgatcaagct
    10861 ggcgctcagc gtcatcaccg ccctcggtgc ggtggccatc ctcttcatcg gcggctggct
    10921 ggtcctccag ggccggagcg acgtcggcac ggtggtggcg agcctcagcg gcctcgcacg
    10981 catcgacggg ccctggcgca cgctgatcca gttcttccgc caggccagca ccgtccgggt
    11041 caactacgag atgctgaccc gggcgctggc gccgcgcggc tgagcgcgtt cctgtcggct
    11101 cttggcgccc gccgccgcct cgcctataga cgatccgtcg agcgcaacgt cgagcgaaag
    11161 gctgcgaagc catgcaacag atatcgaaga cgcggcgagc cctgacgggc ggtgccgtgc
    11221 cggccctggc cctcggcctg ctgctggcca ccggcccggc ggcgctggcg cagcagagcg
    11281 gcctctcctc ggccctgcag caggaacgga tggccttcga ggtcgccgac accgacggcg
    11341 acatgcggat cagcccgggc gagttcgccc gcgacgccgc cgccggcttc agctcgctcg
    11401 acaccgatgg cagcctgacc ctgaccccgg ccgagctcgg cgagcacgac ccggccctct
    11461 tcggcccgat cgacggcgac ggcgacggtg cgctcacctt cgacgaggtc atgacctaca
    11521 agatgaaggc cttcgaggcg gccgacacgg acggcgacgg ctatctgtcg ttcgacgaga
    11581 tggtcggggc agccgaggac cagctgggag cggcgcaatg agcgggacca tgagccatcg
    11641 tcggacacct gtcgccatgg cggccctcgc cggcatcctc gccctctcgc tggccgcctg
    11701 cgagggcggc ggcggcggca cgggctcggc cggtgtgggc accgtcggcg gggccgccgt
    11761 cggcgccggc gccggccggc tgctcttcgg caacagcacc accggcatgc tgatcggcgc
    11821 cggcgtcggc ggcctcgccg gcaacatgac cctcgaccgc caggccgagg agcgccgggc
    11881 gcaggagcag caggcggcgc aggacgccca gatgcggcgc cagctcgact tcgagcggca
    11941 gcaggcgctg caggccgagg agacccgccg ccagatcgag gagcagcggc tcttcgagga
    12001 gtggcggcgc acccagaaca cctccgcccc ggccggctga ccggggcagg cgcgccggcg
    12061 gcgcgggctc ggtcccgttc cggcgcgccc agcgggaagg gcggccggaa cggccgcgcc
    12121 tcctccattg cccgggcgaa ggacggccgg ccgagcagcc tggcgcgata ggcccgaagc
    12181 acgggaaacg ccggggcgtt tgggtgcgtc cagtccgcat ggaagagcga cggcgccgcc
    12241 gcgcagtccg ccagcctgaa accgtcaccg gcggcccggc tccgcccggc cagctccccc
    12301 tcgagccagg cacaggcgag ctccagcttc tccaccgcat gcgccagccc ctcccggcgc
    12361 ttgaccgaat cgcccgtcaa cgccccgccc accgcgtgct gcaccgggtc catgacgtgc
    12421 aggtcgaaga accggtccag aaaacgcacg ccctgcgccg ccagcgaatc ggcaggcagc
    12481 aggcgaaccg gccccggatg cacgagctgc agctactcga cgatgatgct cgtctcgacg
    12541 acctggcgct cgccatccac caggagcgga aacttgcgca acggccagcg ccgccgccac
    12601 gccgccacat gcgccggcct atccgggccg atgcagcgaa actcgaaggg cgtgccgttc
    12661 tcgtagatcg cgatgagcac cttctgcgtg taggacgaga agggatgtcc atagagcgcg
    12721 agcgacatcg ctggacctcc cgatccggcc gctggtgcct tgccgacgac cccaccttat
    12781 cggtatggtt gaatagggtt gagaaaggat gtggggagac acgtctcccc acgccccact
    12841 ggtttttttg ggagtggtgt cgacatgtcc acgaggatcg acctgccacc ggagctcgaa
    12901 gccttcgccc gcgactgcgt gaccagcggt cgctacagcg acaccagcga cgtcatccgc
    12961 gccgccctcc gccggctgca ggacgccgag gcgcgccgaa gcagcttcaa tgccatgctc
    13021 gccgaaaccc gcgccgaagc cgagcgcgac ggaaccttcg atctcgacac cgtgctcgcg
    13081 gaagctgacg cgatcatcga gtcccgatcg ggtgctcaat agagtttggg gaaggcgcag
    13141 ctcagcccgc gcgcccgtcg cgacctgctc gctgcagtcg cctggatcgc tcgcgacaat
    13201 ccacccgcgg caagggcatt gcgagatgcc gtggcccgcg cttcggaacg actgtcgctg
    13261 cttccgagaa ccggcaccga acgcccggac atcgccgatc cgcccgtccg cttcctcgtc
    13321 ctgacgggct ttccttatct tctcgtctat gatgcggaaa gcgacccgcc gctgattctc
    13381 cgcataatcc acggcgcgcg tgatctgcct ggaactcttg gggaatgaag agtgccggaa
    13441 gatgcggcga ggttgatctg ccctgcgccc cccggttgtt gtcagggtcg ttggcccatc
    13501 acgatcgtga ccttcaccag cgtggcggcg gaaacgaggc gtgtctgatc ctgctcgatc
    13561 cggcgcgtga aaccagcccg ctcgggctcg tcgagcagaa tggccagaat ggccgagctg
    13621 tcgagcacca tcagccgggc aggccgcacg catcgtagcc gaggatctcc tcggcgctgc
    13681 gacggtccag atcgggcaga tcggcacctt cccgggcaag cgcatcgagc cggccggcga
    13741 gcccgaccgc ctcgccacgc ttgcgccgct cgcgcgccag ccgctcggcc agggcccgcc
    13801 gcacggcctc ggtcaggctc tcgcccgtga gccgcgccac ttcccgcgcc agccgatcgg
    13861 tctccgcatc cttcagactg agtgccatgc agccgacctt ctagaaaacc ggacgttttt
    13921 atagactgac caccgcagcg tgtcgaccgc cgccaccgag cggcgacagg tgtcggcgcc
    13981 ggcccaggcg gctcgacgag ccaagaaata acaatgaaga agggagctgt cccacttggc
    14041 tccatcgccg gacctcccga tccggccgca tgtgccttgc cgacgacccc atcctatcgc
    14101 tatggctgaa caagaataag aaaggatgtg gggatacacg tctccccacg ccccaccggt
    14161 tgttgggggt cgaacgatga gatgccctgt ctgcaagaac ggcgagagcc gtcccggcac
    14221 cgttactctc accttggtac gcgtcccgct gaccatggtc gtccgccacg ttcccgccca
    14281 gatctgcgac gactgcggcg aggaatacat cgacgagacg gcaacgcaac aggcgctgga
    14341 ccagaccgag gcctccgcgc gacaaggcgt tcaccgtcga ggttcgcgag tttaccgcat
    14401 ggtagcgtat tggcggcaac tgaaagccgt ttctcgaacc tcttcactgg tccaacgtct
    14461 acacggcgat cctcgcaggt tgacaaccac ggaaccgacc cgggtgtacc gcgccaaaag
    14521 cggtgtgaaa tcgttaagac attaattgcc ttccaaagtc gacggggacg agccaatgag
    14581 gttgataatc accgacgtca cggaaatgca cggcggaaac tattgtgtcg ccggctggga
    14641 cccccaggct cgacgaatgg tgcgaccgct gccaagcggc gcgaactgga cggcgggact
    14701 tctgcagcaa caccgcgtcg ttccaggtgt ttcaatcgat gtaactccta cgggccagca
    14761 ccaccaaaat gcgttaccac accgtaccga agatttgcct gtggatcgag caactatcca
    14821 gcatgtgaat tccggcccaa taaactggtt cgccgcaggc gctcctttga cacatgcaac
    14881 tgtgtcagac gcattctccg gccacatcgc ccacaacagc atttggaaca atgttaggca
    14941 gggcgtctat gtgaaagtag gaacccaaac aggatcgctc gcagcagttc ggctcggaag
    15001 gaatgccgtg cagttcctcg aggagttcaa taaactccga gctaagctga acgacggtgg
    15061 gacgaattac aaactgcctg tatcgagcct tgcgctcaaa tctgcgtggc agcagggcgg
    15121 ccttgtggca gtacaacagg cgttgccgac gacccaacag ttccacgtac gtctcggcct
    15181 agcgcgtgcg ttcggtaatc cgccggaaaa gtgttatttg atggtgaacg gaattcatgg
    15241 ttagcgatat gtctcagccg accatattta gcatcgggca ttcgacgcat aattacgagt
    15301 attttttgga acttcttcgg aatgccggtg tgaccgcgat cgccgatgtg cggacatcgc
    15361 catattcacg acatttcccc caatttagcc gggttttact caaggacaag ttgcaggtgg
    15421 acggcattgc gtattcgttc cttggaaagg agctgggtgg gcggccgacg ggagataagt
    15481 actattgcaa tggggtcgcg gactatgagc gcatggccca atcgcctgac ttcgcacgag
    15541 gactgcaacg agtgctggat ggcgcccgaa aatacaatat cgcgctcatg tgctctgaac
    15601 acgagccgtt ggattgtcac aggtgtctgc tcgtaggcag ggcgttgacc gcatgtgagg
    15661 tggacctaaa acatatcctc actgacggta ccattgagga ccatcgcaag atcgaagagc
    15721 ggctcctcaa gtccgaaggt aagcatggcg acgatctgtt tctgtcaccc gtcgagcggc
    15781 tcgctgaggc ttaccggaaa cgtgcgcgca aggtcgcgta cgctgagcag cccgatggat
    15841 ccgcaaccca tagtacaatg gaatagatag tgacccgttt caaagtaaat actattggat
    15901 ttaccaaatc aacggccaag aatttctttg ataggctctt aaaatctggg gttagaaaag
    15961 ttgttgacgt gagactgcat aacacgtccc agctttctgg ctttgcgaag gctgacgacc
    16021 ttgcttactt tctaatgaaa ataggaggca ttgagtacgt tcatcaacct ctgctggcgc
    16081 cgaccgatcc gatgcttaag gcgtacaaga aagaaaaggg cgactggggc acatacgaag
    16141 agcgctttct cggccttatg gcggaacgcg aggttcagaa gcgcttcaag ccggagatgt
    16201 ttgataacgc atgccttctt tgctctgagg acaagccgca ttattgccac cggcgcttgg
    16261 tctgcgagta cctgaaggac gaatggaacg gcgtgctgga cgtacatcac ctgtgaattc
    16321 caagctgtgg gaaatttcgg ggcacgggac aattttgatg cctccgggcg cgggccgcca
    16381 tggacacggc ctcgccgcat cgcctccatc accaatgctt gtacctgcct ccggaagagt
    16441 ggcttgcgga cctcgtctag gactcctggg gactcctggg gacacctccc cttttaacgt
    16501 ttgcgccatc gcctcgacat tcacccgtcc caaaaaccgg aggggccggc ggggaggcga
    16561 gccttcccgc catcttcgta ccggccaatc ccgagcgcgt cggtcgcccc gtgggtatca
    16621 cgaatgctta tggctgctcc ccggagaccc acccgagagc gtcgtttgac gtccggtgag
    16681 gccctgggcg gcttgggttg gcgcgcccga tgcgacattc cggctcgggt tgtcgcggat
    16741 tctgcaacag cgcctgccga agtgttgcag attccgcgac ataacgattc gtgatggcgc
    16801 atctggcgcg acattcgccc ggaaatgtcg cggattccgc gacacgccgc cgcgaacgaa
    16861 cccggggaat cgacgatcga acccgggatt cggacgttcg aacccaagat ttcgacgaac
    16921 gaacccgggc cacggccaac gacccgggga ccagcaagtc tttgcggatg aagagttttt
    16981 cgccccattc cgatcaccga tgcgtggtgc cgtggcgaga aaagcgaaaa ctgccgatcg
    17041 aacccgcacc cgccccgccg cggccttgca ttcgacaccc aaataggcta taaatcctag
    17101 gtacgccgcc cctcacacgc cccccgcgcc cgccttgcct ttatcggcgc gcgcctcggg
    17161 cctgtcgtcg tggaaggagc cgaaccgttt gtcgagcggg accatgccgt cggcgcaggt
    17221 cacctcgaaa tatgtgtggt ggaggtaacg ggcgaaacag gaggtgctga cgccttcccg
    17281 tcggccacca ccacgcggtc gaacccggca tggccccggg ccgcggcgaa aacccgttgc
    17341 ccgacccgcc gcgcggtgtc accttcgacc cgcaaggccg gggaatcgat ggcggcatga
    17401 tgcgggcggt gacaacctat gtgcgctggg tcgacgcgct caaccgcgtc gtcggccgcc
    17461 tcacgatgct gctcatcttc gtcatgatgg gtgtgctgct ctactcgtcg atctcgagga
    17521 gcgccttcaa cctgccgctg atctgggtcg tcgagatgtc ccagttcatg ctggtcgcct
    17581 acttcctgct gggcggcggc tactcgatgc agcagggcgc ccatgtccgc atggacctct
    17641 tctatgccaa atgggcgccg aggaagcgcg ccttcaccga taccatcacc gccttctgcc
    17701 tgatcttcta cctcgtcgtc ctcatgatgg gcggcctctc ctcgacccta tacgcgctgg
    17761 aatacgggca gaagaactac tcgtcctggg cgcccccctt ggcccccatc aagatcatca
    17821 tgtgcatcgg catcgccctg atgctgctcc aggccgtggc cttcctcgtg cgcgacatcg
    17881 cccggctgcg cggcatcgag ctgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnctgac cctgccgctc ttcgtctaca tgggctacgt gctggccgag agcggcatcg
    18061 ccaacgacct ctacgacatg ctgcacgtct ggtccggcgg cctcaagggc gggctcgcca
    18121 tcggcaccgt cgtgctcatg gtcatcgtct cggcgatgaa cggcctctcc gtcgccggcc
    18181 tcgccatcgg cgccaccata gccctgcccg agatgctgcg ccgcggctac gacaagatca
    18241 tggtgaccgg cgtcatccag gccggcagct ccctcggcat cctcgtcccg ccctcggtcg
    18301 tgctcatcct ctacggcatg atcgcccgcc agcccgtcac ccagctctgg ctcgccggcg
    18361 tcctcccggg cctgatgatg gccggattct tcatcctcta catcgccgtg cgctgccgcc
    18421 tcgacccgtc cctcgccccg cccttgccgg cggccgagcg cgacattccg ctgcgccaaa
    18481 agctcggcct gctgcgcgcc ggcatcctgc cgctcctcat cttcttctcg atgaccgggc
    18541 tcttcgtcgc cggcttcacc agcctcgtcg agagcgccgc ctatggtgct gccgccgcca
    18601 ccgtggccgc cgccgtcaag cgccggctca cgatcaaggt gctcgaggag acggtcagga
    18661 agaccctcgc catctcctgc atgttcctct ggatcatcat ggccgccttg tgcttcggcg
    18721 ccgtcttcga cgggctcggg gcagggcggg cgctgcgggg cctcttcctc gacgaatggc
    18781 acctctcgcc ctggaccgtg ctgatcatga tgcagctcag cttcatcatc ctcggcatgt
    18841 tcctcgacga caccgccatg ctggtcatcg tcgccccgct ctacgtgccg ctcgccatcg
    18901 ccctcgattt cgacccgatc tggtacggcg tgctctacac cgtcacctgc cagatcgcct
    18961 acataacccc gcccttcggc tacaacctct tcctgatgcg cgccatggcc ccgccggaga
    19021 tcagcctcgg cgacatctat cgctccattg tgcccttcgt cggcatcatg gggctcaccc
    19081 tcgtcatcct catgcttttc cccgagatcg ccctctggct gccatcgtgg tatgccggac
    19141 gctgagccgg cggcgaaaag atcccggccg cccgacagtc aaggaggacc agaaatgacg
    19201 atcaagaatc cccggcgtcg cgacttcctg ggcaaggccg gcgccaccac gctcggcgcg
    19261 ggcgcggttg ccgccgcctc gaccttcccc gccccctacg tcaaggcgca aggcccgatc
    19321 cgctggcgcc tgcagaccta tgccggcccg gccctcgccg aacacgtcat caagccctcc
    19381 atcgaccagt tcaacaaggt cgccaatggc gagatggaga tcgagctcta cttcgccgac
    19441 cagctcgtcc ccacgcccga gctcttccgc gccgtgcagc agggcaccat cgacgccgtg
    19501 cagagcgatg acgacagcat cggtgctccc gtcgacgtct cggtcttcgg cggctacttc
    19561 cccttcgcgt cccgctactc gctcgacgtg cccaccctct tcaaccactg gggcctgaag
    19621 gagatctggc aggaagccta cggcgagatc gacggcgtca cctggctctc cgccggctcc
    19681 tgggacccct gcaacttcgt cacccgcgac cccatccgca gcgtcgccga cctcgaaggc
    19741 aagcgcgtct tcaccttccc gacggccggc cgcttcctca cccagttcgg cgtcgtcccc
    19801 gtcaccctcc cctgggagga catcgaggtg gcgctccaga ccggcgagct cgacggcgtc
    19861 gcctggtgcg gcatcaccga ggcctacacc gtcggctggg ccgacgtcac cagctactac
    19921 ctcaccaaca acatctccgg cgcctgggtc ggctcctact tcgccaacac cgaacgctgg
    19981 aacgagctgc ccgagcacct caagggcctc ttcaccctgg ccatggacag ctcccactac
    20041 taccgccagc actggtactg gtggggcgaa gcctactacc gcaccaccgg cgacaagctc
    20101 gagctcacca ccatccccga ggaggaatgg agcgccatcg aaggtgccgc cgaagtcttc
    20161 tacgaggaaa tcgccgccga atccgaacgc agcgcccgca tcatccagat catcaagaac
    20221 taccgagctg cgatgcacaa agccggcccg ccctaccggt tcagctgatc gggaaaggga
    20281 agacccgtgg ggaggcacgc ctctgtatgg accggagaca tcattaacag ctgtacggag
    20341 acatgggtga cact
//
LOCUS       DAOTFI010000066        20219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3206_length_20219_cov_5.994644, whole genome shotgun sequence.
ACCESSION   DAOTFI010000066 DAOTFI010000000
VERSION     DAOTFI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 20219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20219
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_20219_cov_5.994644"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..899
                     /locus_tag="P5558_08560"
     CDS             132..899
                     /locus_tag="P5558_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HRY24398.1"
                     /translation="MKTDDVRASAFAMPLTSPSCPKGPCSFVITCRTDPEALRALVRE
                     PLAVGEPIVRHEFIAMPVSTGFGSCGDSGQVIPVRFQGQEGAWVHAMHLKDDAPIAGG
                     REIRGFPRKPAEPALRVEQDTLVGTLDLGSVRLATGTMGRKHKALDPAGVLASFEAPS
                     FLLKIIPHVDCTPRVCELVRYFQQDIRIKGAWSGPAGLELHDHALTPVAALPVPATLS
                     AVHVLADLTRRLGEVVHDYLAAAWTGPRQPITWGNTT"
     gene            896..1678
                     /locus_tag="P5558_08565"
     CDS             896..1678
                     /locus_tag="P5558_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HRY24399.1"
                     /translation="MNLKDKVCIITGAASGIGKGIAERFVADGARVAIADLEADAADR
                     AAAELTGKGPGSAIGVTMNVTDEAQVNAGVARVVEEWGRIDVLVSNAGVQIVHRLENF
                     PFADWKKMLAIHLDGAFLTSKACLPHMYKEDGGSIIFMGSVHSWEASPLKSAYVTAKH
                     GLLGLSRVIAKEGAAHGVRANVICPGFVRTPLVDKQIPEQARDLGISEEEVVKKVMLG
                     ETVDGEFTTIADVAEIAWLFAAFPTNALTGQSLKVSHGWSMT"
     gene            1726..2619
                     /gene="folD"
                     /locus_tag="P5558_08570"
     CDS             1726..2619
                     /gene="folD"
                     /locus_tag="P5558_08570"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRY24400.1"
                     /translation="MARLIDGKAAALALRTEVARAVEGFVAERGLRPGLAVVIVGDHP
                     ASRAYVRTKTRMAEAAGIAARLIELPADIQTAALLERIGELDADPAVHGILVQLPLPE
                     TIDEAQVLLAVSPKKDVDGFHPANVGALFSATRPDPDRLLVPCTPLGCLHLLRQTLGA
                     EGLRGQEAVIVGRSNIVGKPLAALLLAADCTVTVAHSRTRDLADVCRRADILVAAVGR
                     PGLVRGSWVKPGAAVIDVGINRIEAEDGGSRLVGDVAFAEAEQVAGAITPVPGGVGPM
                     TVACLMRNTLTAARLQSGAGG"
     gene            complement(2692..3426)
                     /locus_tag="P5558_08575"
     CDS             complement(2692..3426)
                     /locus_tag="P5558_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY24401.1"
                     /translation="MRALVIEDDPVSAKLIETALRTENIVFEPTDRGEEGIELAKLYD
                     FDIIILDLRLPDIDGYEVVRRLRASRVQTPVLILSGRSDPTDKVRGLTNGADDYLTKP
                     FNKAEMLARIHAIVRRAKGHSESVIRTGKLLVNMDSRSVEVDGQRLHVTGKEYNILEL
                     LSLRKGTTLTKEMFLDHLYGGIDEPELKIIDVFICKLRKKIATGTGGQHYIETVWGRG
                     YVLKDPEPRSETVIPPETTTATAAVA"
     gene            3594..4988
                     /gene="fliI"
                     /locus_tag="P5558_08580"
     CDS             3594..4988
                     /gene="fliI"
                     /locus_tag="P5558_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HRY24402.1"
                     /translation="MSEAEDAAGGGPSTAPLLGEDEIRAALGAVRGLETWRCFGRVAA
                     CRGQVVRAAGLGGLVGIGDGCVVERPGGAAVATEVVGFDGPATLLAPYGEIAGITHGA
                     RVRVERRLEGLRPDASWRGRVVDAFGHAQDGGPPLRPGRRIYAVQAPPPPAGQRAALG
                     GRLALGVRALDLFAPVCAGQRLGIFAGSGVGKSSLLSMLAKAATADVLVIGLIGERGR
                     ELKEFLDHTLGAEGRARSVVVVATSDQPAMTRRRAAYATLAIAEHFRDQGANVLCLMD
                     SVTRFAMALREIHLAAGEPPTSKGYPPSVFTELPRLLERAGPGREGSGTITGLFTVLV
                     DGDDTSEPISDAVRGILDGHVVLDRRIAEAGRFPAMDVLKSLSRTAPGCYAPAERKLV
                     AAARRLMQSYSEMAELIELGAYRPGSSPLVDEAVARRPALEAVLAQGIEEAQGAGDPF
                     ARLAEALDVDWPAG"
     gene            4991..5416
                     /locus_tag="P5558_08585"
     CDS             4991..5416
                     /locus_tag="P5558_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24403.1"
                     /translation="MKRDPLAILARLERQTLDARRGAMVEAQGRQDRLRGRAARDKAA
                     WSEAVEQAVTAEAELDLWGALSRGTRHVLDRALAERDAQATELAEARAALQASLVELK
                     RLDVLTERRRQRDHAARALRERIELDDLAILRHRREKGG"
     gene            complement(5450..5764)
                     /locus_tag="P5558_08590"
     CDS             complement(5450..5764)
                     /locus_tag="P5558_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY24404.1"
                     /translation="MAKLKVTDRDGVQHELEGEIGWSVMEIIREADLPIEAACGGCCA
                     CATCHVYVDAGTLAKLPPADADENMMLDEGFEVRDNSRLSCQIKFSEELDGIEVELAP
                     EY"
     gene            complement(5765..6808)
                     /locus_tag="P5558_08595"
     CDS             complement(5765..6808)
                     /locus_tag="P5558_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015088973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY24405.1"
                     /translation="MTGSPSTQEPIQTDMVIIGAGPIGLFAVFEAGLLGLKCHLVDNL
                     DKIGGQCAELYPEKPIFDIPAVPRCTGQELIDRLLEQAKPFEPVFHLDQQASALQRLE
                     DGKWRLETTAGTVFETPNVVIAAGAGSFVPRRLPLPGAEDFEGKSLYYAVRRMEDFRG
                     RHLLIAGGGDSALDWLLNLEPLTASIGILHRRDEFRAAPDSVQRMRELVESGRARLLL
                     GQVAGLEGTGGQLEAVRISTPDGEIRHECDALLALFGLKMELGPIAEWGLNIERNLIH
                     VDTETFETNQPGLFAIGDIITYPGKLKLILSGFHEAALMCQKAFKNAHPDQRLVFRYT
                     TSSSDLHKKLGVA"
     gene            complement(6922..7947)
                     /locus_tag="P5558_08600"
     CDS             complement(6922..7947)
                     /locus_tag="P5558_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HRY24406.1"
                     /translation="MDEPRTKKLMNEAGDIVREMVEGVAAVHPGIVRLHPEDWRVVLR
                     AAGEPAAKVGIVVGGGSGHEPAFLGYVGRGLATAAVIGDVFTSPTPEPILTAARAAAG
                     PEGVLFLYGNYAGDVLNFDMAAELAEAEGIAVRTVLGADDVAAAPVERAEERRGIAGG
                     VFLFKIAGAAAAAGWPLVEVERVAKKAVGAMRSMGVALAPCSLPASLRPNFAIAPDEM
                     EIGLGIHGEKGIMSGPLEPADRVTGQLFERLQGELALQPGERCAVLVNGLGATSFMEC
                     HVVARRLLQLLSGAGVLAHHVRVGEYVTSLEMAGLSLTLMRLDDELAGLLEAPAEGIA
                     WSTRPQG"
     gene            8163..9182
                     /locus_tag="P5558_08605"
     CDS             8163..9182
                     /locus_tag="P5558_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRY24407.1"
                     /translation="MRAVFIHAAYDIRLGDLPVPNAGPGQVLVDIEAVGLCGSDLHYY
                     KDGGIGGTQVIVEPFVPGHEAAGRVREAVPERGIAEGAMVAIEPAFPCGACEWCHSGR
                     HNICPHTLMMGGPPNHGALTRTVALAPGQLYAVPEGFSASTTALLEPLGVCIHAVDLA
                     RPQWFETVAVLGAGTIGLGILQLLRLAGIAEPIVVDPVGYRREKALELGAATAVASVA
                     EIDGLTAGRGADLVLEATNSPAGFADATAAAAIGGRVVIVGIPDGNAYTFEAATPRRK
                     QLQVTFSRRMNHVLPRAIRLVAEGRLDPETWVSHRIGLDGVPEALDMLANYRDRALKV
                     VVQPD"
     gene            9401..10936
                     /locus_tag="P5558_08610"
     CDS             9401..10936
                     /locus_tag="P5558_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRY24408.1"
                     /translation="MDQDLVIGIDSSTTATKATAWDRSGNLVAEGRAAIPLANPAPGF
                     YEQDAQDWWRSLCAALAELGRRVDTHRIAALAIANQRETFVPVDAEGEPLRPAITWLD
                     ERGRDDVVLLSKKLGRERLRQITGKTPDPTPCLYALHWMLRKEPGLYRRTVRFLDVHG
                     FLVHRLTGLYRTSWASADPTGLYDLAAKAYSEPILDALQLEEKQLATALQPGTVLGEV
                     TGEAAEETGLQPGTLVIAGGGDGQAGGLGTGTLQPGRAYLNLGTAAVSGVYSATYATD
                     PAFRTLTSVSGEGYILELCVRTGTFLTDWFVKNVFAVDPVAEPGIYDALEAAAGKLPV
                     GADGLLLQPYWSGVMNPWWDQDARGLMLGLSSAHGQAHLYRAMMEGLALDEAMGIAEI
                     ERVTGERVDELVTIGGGARSELWCRIIADATGKVVARPATVEATALGAAIAAAKACGW
                     FADAAAAAAAMTGRIEDRIEPEPANLALYGELLEIYRTLYPATREAQAALAAFRHRTA
                     AAT"
     gene            10933..12243
                     /locus_tag="P5558_08615"
     CDS             10933..12243
                     /locus_tag="P5558_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRY24409.1"
                     /translation="MSSGDALIQDFVAGRYRAIETGLPVQCPIRAIVTAPSLAGREAE
                     LLRGIGLAKNGSGGTIGVVSDENTRDVLGDRVERALPGCRSVVLDHPHADEATAADLR
                     ERTRHCDALVAVGSGTLNDLCKHVTHGDGRPCAVFATAASMNGYVTGTASITRGDFKH
                     SLPSHAPRGVFLDLQVLAAAPPRLTRAGLGDALCRGTAQIDWLLSHLLLDTAYAESPF
                     AMQRVDEPKLLAAAPRLLDGDLAAMATLVHVLTLGGLGVVVTGTSHSGSMGEHGISHY
                     IDMFARPHPGSLHGAQVGVATLTMARLQAQLLAEEAPPVLRASRIDDAALAERYGAQA
                     AACRKALLAKALTGERLAAANARLAESWADWRHRLAALAIPVDRLEAALLSIDAARTG
                     ADLGLEPAAYRAAVAHAHEIRDRFGFLDLAAHAGRLEAFAAGEG"
     gene            12251..13303
                     /locus_tag="P5558_08620"
     CDS             12251..13303
                     /locus_tag="P5558_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY24410.1"
                     /translation="MGTVLVLGAGVMGSAFTLPLADAGTTVRLVGTHLDTALIDGLRA
                     TGVHQRLKAPLPAAVETFHHHELALALDDSVELVVLGVSSAGIAWAIDRLRPLLSAKR
                     PVLLLTKGLAADGRRIGILPEPVEAALGCPTGAVGGPCIAGELAVRRATSVVMSFADP
                     ALIRQVLALTAAPYYHARASADLVGTEVCAAFKNFMALGIGAARGMADVAGPADNGAA
                     MHNPAAGLFTQAVLELGRLNALLGGTPASVHGLAGTGDLYVTVQGGRNSRMGRLLGAG
                     WVYSRAKAERMAEDTVEGAELALAVGPTLRAMLDRDDLRADDLPLTLAILDAIGEDRP
                     LVLPWAAFHQRGPGAA"
     gene            13293..14270
                     /locus_tag="P5558_08625"
     CDS             13293..14270
                     /locus_tag="P5558_08625"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HRY24411.1"
                     /translation="MPPDRPDPLPESASFDRVEQLRTRIAWLYYVEGLTQSDIADRVG
                     LSRVRVNRELAVCRERGIVQIRINSRLATCVALERRLIRHFGLKDAVVVPTPEDPALL
                     PGIIGMAAGQYVSDRLAAGMSIGVGWGRTLLGSLKAIERRNVPGLSVVSLMGGLTRAS
                     AINTYETASRFADLFGADCYYLAAPTFTSSPESRATFIEQAAIKDVLDRARKVDLALV
                     SVGGLGPDATIRRLDMLSADELDSLVEAGALGDSLGHFIDAAGRLVDHPINHRVIGLA
                     PAELSDVPTAILASGGPDKDRVIGAVLGARFLNVAIIDEATAERLLARA"
     gene            14323..>15386
                     /locus_tag="P5558_08630"
     CDS             14323..>15386
                     /locus_tag="P5558_08630"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY24412.1"
                     /translation="MPSDVERELVDWLIGAALDLTDEQTLFAGLCDRLSLAGVGITRA
                     YLASEVLHPLIGARGFMWQGEGAVREDYGHDGIGVVDPNWRRSPFFHMLDRSLDRLHV
                     RLDASLEPRRFPLLDRLAAQGCTDYLALGRRMVSTAALGGQDGILTSWTTMRPGGFEA
                     GETALIERCMAPLALAFKGFRAVDTARTLACTYLGQDAGSRVLAGEIRRGEPVTIEAV
                     LWYSDLEGFTRIADTTPPDRLMALLNAYAEVLVSVVDGRGGDVLKFMGDGILALFRGD
                     DAAARALDAAIAAQRAVGELNATRRAAGEPVTEFYLGLHRGDVLYGNIGSRERLDFTV
                     IGPAVNEVARIEAMCRSLDRR"
     assembly_gap    15387..15486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15487..16908
                     /locus_tag="P5558_08635"
     CDS             <15487..16908
                     /locus_tag="P5558_08635"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRY24413.1"
                     /translation="VFFLLRVGGITIRDRVRSTLEVESGSNDPMAIFLVVVLLDLILS
                     GAGLDTLGPSLLHAFALQMGLGLAAGLAGGWLIVKGINLIELEPGLYPIAVLGGALML
                     FALTGLLGGSGFLAVYVAGLVAGNMRIRARPHLHRFQDGLTWLAQIVMFLLLGLFATP
                     STFGEIALPVIVLAILLVVLFRPLAVTLLLLPFGFRRNEVAFISVVGLRGAVSILLAI
                     MPIMARLEHGQTLFNAAFLIVLVSLLTQGWSIRPLARWLGVVVPPRQGPVDKVELELP
                     GTAHHELVVYHITEGSPVARGHRLPRWARPSLVVRGGRSLSVHQAGHLQPDDYVYIFT
                     APSFIHLLDRLFASRAALDPADIDFYGELPIVPDAPVAALAATYGLAIETDPTTPRIA
                     DYLEQRLGGHPEPGDRLTLGAVDLIVRSVDDAGRIAEIGLSFRPIEEATRQGGTLARM
                     RRLLVGRPQAAPIAEPEEAPKAP"
     assembly_gap    17009..17108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17109..17524)
                     /locus_tag="P5558_08640"
     CDS             complement(<17109..17524)
                     /locus_tag="P5558_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24414.1"
                     /translation="MSGGFEGKVAIVTGGTQGLGEATARLFVERGARGVVICGRNAEK
                     GERVRADLAAAGAEAVFVAADLGDLDQARRVTKAADDAFGRVDCLVNVAAITDRGTIL
                     DTSPELYERMFAINVRAPFFLMQDTATIMRREKIAGT"
     gene            complement(17597..17776)
                     /locus_tag="P5558_08645"
     CDS             complement(17597..17776)
                     /locus_tag="P5558_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24415.1"
                     /translation="MTTDRQLAGAYDPVHVSAIMTEARRMRNAMILGFLRRLFLRHGE
                     RRATPAAGGLAASGT"
     gene            complement(17903..18742)
                     /locus_tag="P5558_08650"
     CDS             complement(17903..18742)
                     /locus_tag="P5558_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HRY24416.1"
                     /translation="MSRAGERIAALPMYDLPELRPATDRLWQAVALRLRTAGVPGVPA
                     GLWRGDDLIGLWTDPGLLLAQACGLPFTTLLAGRVRFVATPAYAISGCEGGAYRSWIV
                     VREAEPHDSLAELEGSRAAVNAPHSQSGANALAAATVPFAAGRPFFSGIVETGAHAAS
                     LAAVREGRADCAAIDCVTFALLARHRPAAVAGLRVLAATPPAPALPLVTAAGTGRRTV
                     AALRRALAAAIADPALAATCDALGLAGIAVGDGRAYGRIRAMHRAARTRGCARLASFM
                     IKG"
     gene            18872..19141
                     /locus_tag="P5558_08655"
     CDS             18872..19141
                     /locus_tag="P5558_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24417.1"
                     /translation="MTIDSGTVLEIGENLVEEIDGFEMAFPAGAYRALPVPSAALDSD
                     QGGATNADADRYLLLPVGRERVHPISAEGLGQLVELDSVTVARAD"
     gene            complement(19158..20057)
                     /gene="iolE"
                     /locus_tag="P5558_08660"
     CDS             complement(19158..20057)
                     /gene="iolE"
                     /locus_tag="P5558_08660"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HRY24418.1"
                     /translation="MAIRLGIAPIGWSNDDLPELGGDITLEQCLKEARQAGYSGVEKG
                     GKFPMDPKLLKPIMADHQLELVSGWFSGRLLEVTVEEEKSRIKEQLALYQAMGCPVMV
                     YAETVGTVQGLIDTPVSQRPKLTPDQIRRYGEKLTKFAEFLQAEHCPMTFHHHMGTVI
                     ETEEEIDLLMESTDAPVGLLLDTGHLTFAGGDVLATTERWGRRINHVHTKDIRKDVLK
                     KLKAEDWSFLKGVLEGVFTVPGDGSIDFGAFMKSLKTIGYSGWVVIEAEQDPKKANPL
                     KMAEIGMAALSEAAAAAGLSIAS"
BASE COUNT         2700 a         7343 c         6989 g         2987 t
ORIGIN      
        1 cgccgtggag cgcacgctcg gccaccggtc gtggaagtcc cggcgcccgc ccaccgacgg
       61 catccggatc ctcgacctcg gcagcgatct cgaaccccat cgccgaaggg cccgctcatg
      121 aagagcgcgc catgaagacc gacgacgtcc gcgccagcgc tttcgccatg ccgctgacca
      181 gcccctcctg cccgaagggg ccctgcagct tcgtcatcac ctgccggaca gaccccgaag
      241 cgcttcgggc actcgtgcgg gagccgctcg cggtgggcga gccgatcgtc aggcacgagt
      301 tcatcgccat gcccgtttcg accggcttcg gctcctgcgg cgattccggc caggtgatcc
      361 cggttcgctt ccagggccag gagggcgcct gggtgcatgc gatgcatctg aaagacgacg
      421 cacccatcgc cggcgggcgc gagatccggg gctttccgag gaagccggcc gagccggccc
      481 ttcgggtcga gcaggacacg ctggtcggca cgctcgactt gggctcggtg cgcctcgcca
      541 cgggcaccat gggccgcaag cacaaggcgc tcgatccggc cggggtgctg gccagcttcg
      601 aggcgccgag cttcctgctc aagatcatcc cgcatgtcga ctgcacgccg cgcgtctgcg
      661 agctggtccg ctacttccag caggacatca ggatcaaggg cgcctggtcc ggcccggccg
      721 ggctcgagct gcatgaccac gccctgaccc cggtggcggc cctgccggtg cccgcgacgc
      781 tttcggccgt gcatgtcctg gccgatctca cccgccgcct cggcgaggtc gtgcacgact
      841 atctggcggc cgcctggacc gggccgcgac agccgatcac atggggaaac acgacatgaa
      901 cctgaaggac aaggtctgca tcatcaccgg tgccgcgagc ggcatcggca aggggatcgc
      961 cgagcgcttc gtcgccgatg gcgccagggt ggcgatcgcc gacctcgagg ccgacgccgc
     1021 cgatcgggcg gccgccgagc tcaccggcaa ggggccggga tcggccatcg gtgtcaccat
     1081 gaacgtcacc gacgaggctc aggtgaacgc cggtgtcgcc cgggtcgtcg aggaatgggg
     1141 ccggatcgac gtgctggtct cgaatgccgg ggtgcagatc gtccacaggc tcgagaactt
     1201 cccgttcgcc gactggaaga agatgctggc gatccatctg gacggcgcct tcctcacctc
     1261 gaaggcctgc ctgccgcaca tgtacaagga ggacggcggc tcgatcatct tcatgggctc
     1321 ggtgcattcc tgggaggcct cgccgctcaa gtcggcctat gtcaccgcca agcacggcct
     1381 gctcggtctc tcccgggtca tagccaagga gggcgccgcg cacggtgtgc gcgccaatgt
     1441 catctgcccg ggcttcgtcc gcacgcccct ggtcgacaag cagatccccg agcaggccag
     1501 ggacctcggc atctccgagg aggaggtggt caagaaggtc atgctcggcg agaccgtcga
     1561 cggcgagttc accacgatcg cggacgtggc cgagatcgcc tggctcttcg ccgcctttcc
     1621 gacgaacgcg ctgacgggcc agtcgctgaa ggtcagccat ggctggtcca tgacttgagc
     1681 atccgacggc aaacggcgag aagcacggga aaaaagggga gtggcatggc gaggctcatc
     1741 gacggcaagg cggcggcgct ggccctgcgg accgaggtcg cccgggcggt cgagggcttc
     1801 gtggcggagc ggggcctccg gcccggcctc gccgtcgtca tcgtcggcga tcatccggcg
     1861 agccgcgcct atgtccggac caagacgcgc atggccgagg cggcgggcat cgccgcgcgg
     1921 ctgatcgagc tgccggccga catccagacc gctgccctgc tcgagcggat cggcgagctc
     1981 gacgccgatc ccgccgtgca cggcatcctg gtccagctgc ccttgccgga aacgatcgac
     2041 gaggcgcagg tgctcctcgc cgtctcgccc aagaaggacg tcgacggctt ccacccggcc
     2101 aatgtcggcg ccctcttcag cgccacccgg ccggaccccg accggctgct cgtgccctgc
     2161 acgccgctcg gctgcctgca tctcctgcgc cagaccctgg gggcggaagg gctcaggggc
     2221 caggaggcgg tcatcgtcgg ccgctccaac atcgtcggca agccgctggc cgccctcctg
     2281 ctcgccgccg actgcaccgt cacggtggcg cactcgcgca cccgggatct cgccgacgtc
     2341 tgccgccgcg ccgatatcct ggtcgccgcc gtcggccggc cgggcctcgt ccgtggcagt
     2401 tgggtgaagc ccggtgctgc cgtcatcgat gtcggcatca accgcatcga ggccgaggat
     2461 ggcggcagcc gtctcgtcgg cgacgtcgcc ttcgccgagg cggagcaggt cgccggcgcc
     2521 atcaccccgg tgccgggcgg ggtaggtccc atgaccgtcg cctgcctgat gcgcaacacg
     2581 ctgaccgccg cccgcctgca gtccggcgcc ggcgggtagc gggaggtcgc ccgtcgcgcg
     2641 tccatcgggg tcgcgcggcc gctgtccggc gccgtagctc gggcgacggg ctcaggcgac
     2701 cgcggcggtg gccgtcgtcg tctcgggcgg tatcaccgtc tcgctgcgcg gctcggggtc
     2761 cttcagcacg tagccccggc cccagaccgt ctcgatatag tgctggccgc cggtgccggt
     2821 cgcgatcttc ttccggagct tgcagatgaa gacgtcgatg atcttgagct ccggctcgtc
     2881 gatgccgcca tagagatggt cgaggaacat ctccttggtg agcgtcgtgc ccttgcgcag
     2941 cgagagcagc tcgaggatgt tgtattcctt gccggtgacg tgcagccgct ggccgtccac
     3001 ctcgaccgag cggctgtcca tgttgacgag cagcttgccg gtgcggatca ccgattccga
     3061 atgacccttc gcccgccgca cgatggcgtg gatgcgggcc agcatctcgg ccttgttgaa
     3121 gggcttggtc aggtagtcgt cggcgccgtt ggtcaggccc cgcaccttgt cggtggggtc
     3181 gctgcggccg gagaggatca ggaccggcgt ctgcacccgg ctcgccctga ggcggcggac
     3241 cacctcgtag ccgtcgatgt ccggcaggcg cagatcgaga atgatgatgt cgaaatcgta
     3301 gagcttggcg agctcgatgc cttcctcgcc gcgatccgtg ggctcgaaca cgatgttctc
     3361 ggtgcgcagg gccgtctcga tcagcttggc cgataccgga tcgtcctcga ttaccagtgc
     3421 tcgcatggtg tctccatctg tcccgctcgt ccgtacgttg ccgtaacctt aagactggtt
     3481 tccttaaggc tctgttaatg cggcacgcct aaatgtcctt gcgacacggc cggatgcgca
     3541 tgccggcgtg cgacgctgga ggcggggacc ggaacgatgg cgcgaagggc ggaatgagcg
     3601 aggccgagga cgccgcaggc ggcgggccgt caacggcacc gctgctcggc gaggacgaga
     3661 tcagggctgc cctgggagcc gtgcgcgggc tcgagacctg gcgctgcttc ggccgcgtcg
     3721 ccgcctgccg cggccaggtc gtccgggccg cgggcctcgg cggtctggtc ggcatcggcg
     3781 acggctgcgt ggtcgagcgg ccgggcggtg ccgcggtggc gaccgaggtg gtcggcttcg
     3841 acgggccggc gacgctgctc gccccctatg gcgagatagc cgggatcacg catggcgccc
     3901 gggtccgggt cgagcggcgt ctcgaggggc tgcggcccga tgcgagctgg cgcggccgcg
     3961 tggtcgacgc cttcggccat gcccaggacg gcgggccgcc tctgcggccg gggcgccgga
     4021 tctatgccgt ccaggcgccg ccgccgccgg ccggccagcg ggccgcgctc ggcggccgtc
     4081 tggcgctcgg cgtgcgcgcc ctcgacctct tcgcgccggt ctgcgccggc cagcggctcg
     4141 gcatcttcgc gggctcgggc gtcggcaagt cgagcctgct ctccatgctg gccaaggcgg
     4201 cgaccgccga tgtcctggtg atcgggctga tcggcgagcg cggccgcgag ctcaaggagt
     4261 tcctcgatca tacgctcggc gccgagggcc gggcgcgcag cgtggtggtc gtcgccacct
     4321 ccgaccagcc ggccatgacc cgccgccgcg cggcctacgc cacgctcgcc atcgccgagc
     4381 atttccgcga ccagggcgcg aacgtgctct gcctcatgga cagcgtcacc cgcttcgcca
     4441 tggcgctgcg cgagatccac ctcgccgccg gcgagccgcc gaccagcaag ggctacccgc
     4501 ccagcgtctt caccgagctg ccccgcctgc tcgagcgggc cgggcccggg cgcgagggca
     4561 gcggcacgat caccggcctc ttcaccgtcc tggtcgatgg cgacgacacc agcgagccga
     4621 tcagcgacgc ggtgcgcggc atcctcgacg gtcacgtcgt gctcgaccgg cggatcgccg
     4681 aggccggccg cttcccggcc atggacgtgc tgaagagcct gtcccgcacc gcgccgggct
     4741 gctacgcgcc ggccgagcgg aagctggtcg ccgccgcccg ccggctgatg cagagctaca
     4801 gcgagatggc cgagctgatc gagctgggcg cctaccggcc gggcagcagc cccctggtcg
     4861 acgaggcggt cgcccgccgc cccgccctcg aggcggtgct ggcgcagggc atcgaggaag
     4921 cgcagggggc cggcgatccc ttcgcccggc tggccgaggc cctcgacgtc gactggccgg
     4981 caggctgacc gtgaagcgcg atccgctcgc cattctcgcc cgactcgaac ggcagacgct
     5041 cgatgcccgg cggggggcga tggtcgaggc ccagggccgg caggatcgcc tgcggggccg
     5101 ggcggcgcgc gacaaggcgg cgtggtccga ggcggtggag caggcggtga ccgcggaggc
     5161 cgagctcgac ctctggggtg ccctctcgcg cggcacccgt cacgtgctcg accgggccct
     5221 ggcggagcgg gacgcgcagg cgacggagct cgccgaggcc cgggcggccc tgcaggcgag
     5281 cctcgtcgag ctcaagcggc tcgatgtgct gaccgagcgt cgccgccagc gcgaccacgc
     5341 cgccagggcg ctgcgcgagc gcatcgagct cgacgacctc gcgatcctgc ggcaccggcg
     5401 ggaaaaaggc ggctgagggc cggctgcgac cggcctttga agagccggct cagtattccg
     5461 gcgccagctc cacctcgatg ccgtcgagct cctcggagaa cttgatctgg cagctcaggc
     5521 gcgagttgtc gcgaacctcg aagccctcgt cgagcatcat gttctcgtcg gcatcggccg
     5581 gcggcagctt ggcgagcgtc ccggcgtcca catagacgtg gcaggtggcg caggcgcagc
     5641 agccgccgca ggccgcctcg atcggcaggt cggcctcgcg gatgatctcc atcaccgacc
     5701 agccgatctc gccctcgagc tcgtgctgga cgccgtcgcg gtcggtgacc ttcagcttcg
     5761 ccattcaggc aacacccagt ttcttgtgca gatcggagct ggaggtggtg tagcggaaca
     5821 ccagccgctg gtccggatgc gcgttcttga acgccttctg gcacatcagc gcggcctcgt
     5881 ggaagcccga gaggatcagc ttgagcttgc ccggataggt gatgatgtcg ccgatcgcga
     5941 aaagacccgg ctggttggtc tcgaacgtct cggtatcgac atggatcagg ttgcgctcga
     6001 tgttcaggcc ccactcggcg atcggcccga gctccatctt cagcccgaag agggccagga
     6061 gcgcgtcgca ttcgtgccgg atctcgccgt cgggcgtgct gatccgcacc gcttccagtt
     6121 ggccgcccgt gccctccagc ccggccacct ggccgagcag gagccgcgcc cggccgctct
     6181 cgaccagctc gcgcatgcgc tgcacgctgt cgggggcggc gcggaactcg tcgcgccgat
     6241 gcaggatgcc gatcgacgcg gtcaggggct cgagattcag gagccagtcg agagcactgt
     6301 cgccgccgcc ggcgatcagg agatggcgac cgcggaaatc ctccatccgc cggaccgcgt
     6361 agtagagcga cttgccctcg aagtcctcgg cacccggcag cggcaggcgg cgcggcacga
     6421 agctgccggc acccgcggcg atcaccacgt tgggggtctc gaagacggtg ccggcggtgg
     6481 tctcgagccg ccacttgccg tcctccagcc gctgcagcgc cgaggcctgc tggtcgagat
     6541 ggaagaccgg ctcgaacggc ttcgcctgct cgagcaggcg gtcgatcagc tcctggccgg
     6601 tgcagcgcgg caccgccggg atgtcgaaga tgggcttctc gggatagagc tcggcgcact
     6661 gcccgccgat cttgtcgaga ttgtcgacca gatggcactt gaggccgagc agcccggcct
     6721 cgaacacggc gaagagcccg atcggcccgg cgccgatgat caccatgtcg gtctgtattg
     6781 gttcctgggt gctcggtgat cccgtcatcg ctggcccgcc ggactttcgg gtgatgggtg
     6841 ccgccggcac ccggtgggtt cggctagcat cacctttccc cttcggcaag cccgctggtc
     6901 cgccaggggg aggcggcggg ctcagccctg tggccgtgtg ctccaggcga tgccctcggc
     6961 cggtgcctcg agcagcccgg ccagctcgtc gtcgagccgc atcagcgtca gcgacaggcc
     7021 ggccatctcg agcgaggtca catactcgcc gacgcgaacg tggtgcgcca gcacgccggc
     7081 cccggaaagg agctgcagga ggcggcgggc gacgacatgg cactccatga aggaggtggc
     7141 accgagcccg ttgaccagga cggcgcagcg ctcgccgggc tgcagcgcca gctcgccctg
     7201 cagccgctcg aagagctgcc cggtgacccg gtcggccggt tcgagcggcc cgctcatgat
     7261 gcccttctcg ccatggatgc cgaggccgat ctccatttca tcgggtgcga tcgcgaaatt
     7321 cggccggagg ctcgccggca gcgagcaggg ggccagcgcc acgcccatgc tgcgcatggc
     7381 gccgaccgcc tttttcgcca cccgctcgac ctcgaccagg ggccagccgg cggcggccgc
     7441 ggcaccggcg atcttgaaga ggaagacgcc gccggcgatg ccgcggcgct cctcggcccg
     7501 ctcgaccggg gcggcggcca cgtcgtcggc gccgagcacg gtgcggacgg cgatgccctc
     7561 ggcttcggcc agctcggcgg ccatgtcgaa gttgagcacg tcgccggcat agttgccgta
     7621 gaggaagagc acgccctcgg ggccggcggc ggcgcgggcg gcggtcagga tgggctcggg
     7681 cgtcggcgag gtgaagacgt cgccgatcac ggcagccgtg gcgaggcccc ggccgacata
     7741 gccgaggaag gccggctcgt ggccggagcc gccgccgacg acgatgccga cctttgctgc
     7801 cggctcgccg gccgcgcgca ggaccacgcg ccagtcctcg gggtgcaggc ggacgatccc
     7861 tgggtggacg gcggccaccc cttcgaccat ctcgcggacg atgtcgcccg cctcgttcat
     7921 cagcttcttg gtgcgtggct cgtccatggt cgccttttcc ccaaccgcct gatccggccc
     7981 cgaggatcgg ggggaagcgg ccgggacgca aggctcggct cgcccgccct ctggggcgat
     8041 ccgctcctcg cttccaccga agcgcccggc agagaccgct ttgcctcggc gccgatcggc
     8101 gctatcgctc tggcgcggcg cacctgctga tctcgccgcc ttcgagcccc gggtgaccct
     8161 tcatgcgcgc cgtcttcatc catgccgcct acgacatccg cctcggcgac ctgccggtgc
     8221 ccaatgccgg gcccggccag gtgctggtcg acatcgaggc ggtgggcctc tgcggcagcg
     8281 acctgcacta ctacaaggac ggcgggatcg gcgggacgca ggtcatcgtc gagcccttcg
     8341 tgccgggcca cgaggcggcc ggtcgggtgc gcgaggccgt tccggagcgc ggcatcgccg
     8401 agggggcgat ggtcgccatc gagccggcct tcccctgcgg cgcttgcgaa tggtgccatt
     8461 cgggccgcca caacatctgc ccgcatacgc tgatgatggg cgggccgccc aatcacggcg
     8521 cgctcacccg caccgtggcg ctggccccgg ggcagctcta tgccgtgccg gagggcttct
     8581 cggcctcgac cacggccctg ctcgagccgc tgggcgtctg catccacgcc gtcgacctcg
     8641 cccggccgca atggttcgag accgtggcgg tgctgggggc cgggacgatc gggctcggca
     8701 tcctccagct cctgcggctc gccggcatcg ccgagccgat cgtcgtcgac ccggtgggct
     8761 atcgccgcga gaaggcgctc gagctgggtg ccgccacggc ggtggcgagc gtcgccgaga
     8821 tcgatgggct gaccgccggg cggggggcgg acctcgtgct cgaggcgacc aacagccccg
     8881 ccggcttcgc cgatgcgacg gcggcggcgg cgatcggtgg ccgggtcgtg atcgtcggca
     8941 ttccggacgg caacgcctat acgttcgagg cggccacgcc gcgccgcaag caactgcagg
     9001 tgaccttctc caggcgcatg aaccacgtgc tgccacgcgc catccgcctc gtcgccgagg
     9061 gccggctcga ccccgagacg tgggtgagcc accggatcgg cctcgatggg gtgcccgagg
     9121 cgctcgacat gctcgccaat taccgcgacc gggcgctcaa ggtcgtcgtc cagccggact
     9181 gagagcccgt gcggccggcc acatctgcgc aggcccttga caggtggcgg cgctcttccg
     9241 gatatggacg aagattgtgc ggccgaacga acaaaagttc gccgtggagg cgaccatgat
     9301 ccggcaatcc gcgcctgtgg cccgatcagg aaacgcacga cgcccggcag gccgcgccgg
     9361 cggcggctga cggggcaccg ttcggcggca gcgagggacc atggaccagg atctcgtcat
     9421 cggcatcgac agcagcacca ccgccaccaa ggccacggcc tgggaccggt cgggcaacct
     9481 cgtggccgag gggcgtgccg ccatcccgct cgccaacccg gcacccggct tctacgagca
     9541 ggatgcgcag gactggtggc gctccctctg cgccgcgctc gccgagctcg gccgccgggt
     9601 cgacacgcac cggatcgctg ccctcgcgat cgccaaccag cgcgagacct tcgttccggt
     9661 cgacgccgag ggtgagccgc tgcgcccggc catcacctgg ctcgacgagc gcggccgcga
     9721 cgatgtcgtg ctgctctcga agaagctcgg ccgcgagcgc ctgcgccaga tcaccggcaa
     9781 gacgccggac ccgacgccgt gcctctatgc gctgcactgg atgctccgca aggagcccgg
     9841 gctctaccgc cgcacggtcc gtttcctcga cgtgcacggc tttctcgtcc accggctgac
     9901 cgggctctat cgcacgagct gggcgagcgc cgacccgacc ggtctctacg acctcgccgc
     9961 caaggcctat agcgagccca tcctcgatgc cctgcagctc gaggagaagc agctcgccac
    10021 ggctctccag cccggcaccg tgctcggcga ggtgaccggt gaggcggccg aggagaccgg
    10081 tctccagccc ggcacgctgg tgatcgccgg cggcggggac ggccaggccg gcgggctcgg
    10141 caccggcacg ctccagccgg gccgggccta cctcaatctc ggcacggccg cggtctccgg
    10201 cgtctacagc gcgacctacg ccacggaccc ggcgttccgc acgctgacca gcgtctccgg
    10261 cgagggctac atcctcgagc tctgcgtgcg caccggcacg ttcctcaccg actggttcgt
    10321 caagaacgtg ttcgccgtcg acccggtggc cgagcccggc atctacgacg cgctcgaggc
    10381 cgcggccggc aagctgcccg tcggtgccga cgggctcctc ctgcagccct actggagcgg
    10441 cgtcatgaac ccctggtggg accaggacgc gcgcgggctg atgctcggcc tgtcgtcggc
    10501 gcacggccag gcgcacctct accgggcgat gatggaaggc ctcgccctcg acgaggccat
    10561 gggcatcgcc gagatcgagc gggtcaccgg cgagcgggtc gacgagctcg tgaccatcgg
    10621 cggcggtgcc aggagcgagc tctggtgccg gatcatcgcc gacgcgaccg gcaaggtggt
    10681 ggcccggccg gcgacggtcg aggcgaccgc gctcggtgcc gcgatcgcgg ccgcgaaggc
    10741 ctgcggctgg tttgccgacg ctgccgccgc tgccgctgcc atgaccggcc ggatcgagga
    10801 ccggatcgag ccggagccgg cgaacctggc gctctacggc gagctcttgg agatctaccg
    10861 cacgctctat ccggcgaccc gcgaggcgca ggccgccctc gccgccttcc gccatcgaac
    10921 ggctgcagcg acatgagcag cggcgacgcg ctcatccagg atttcgtcgc cggccgctac
    10981 cgggcaatcg agacgggcct gccggtccaa tgcccgatcc gcgccatcgt caccgccccc
    11041 agcctcgccg gccgggaggc ggagctgctg cggggcatcg ggctcgccaa aaacgggtcg
    11101 ggcggcacga tcggcgtggt cagcgacgag aacacgaggg acgtgctcgg cgaccgggtc
    11161 gagcgggcac tccccggctg ccgttccgtc gtgctcgacc acccccacgc ggacgaggcg
    11221 acggccgccg acctgcgcga gcgcacccgg cattgcgatg ccctcgtggc cgtgggctcg
    11281 ggcacgctca acgacctctg caagcacgtc acccatggcg acggccgccc ctgcgccgtc
    11341 ttcgccaccg ctgcctcgat gaacggctac gtcaccggca ccgcctccat cacccggggc
    11401 gacttcaagc acagcctgcc gtcccatgcg ccgcgcggcg tcttcctcga cctccaggtc
    11461 ctggcggccg ccccgccgcg gctcacccgc gccgggctcg gcgacgcgct ctgccgcggc
    11521 accgcccaga tcgactggct cctctcgcat ctcctgctcg acaccgccta tgcggaaagc
    11581 ccgttcgcca tgcagcgcgt cgacgagccg aagctcctgg ccgccgcacc ccgcctgctc
    11641 gacggcgacc tggcggcgat ggccacgctg gttcatgtcc tcaccctcgg cgggctcggc
    11701 gtcgtggtca ccggcaccag ccactcgggc tcgatggggg agcatggcat cagccactac
    11761 atcgacatgt tcgcccgccc gcacccgggc tccctgcacg gtgcccaggt cggcgtcgcc
    11821 acgctcacca tggcccggct gcaggcgcag ttgctggcgg aggaggcgcc gccggtgctg
    11881 cgggcgagcc ggatcgacga cgcggcgctg gccgagcgct acggcgccca ggccgcggcc
    11941 tgccgcaagg cgctcctggc caaggcattg accggcgaaa ggctcgccgc cgccaatgcg
    12001 cggctggcgg aatcctgggc cgactggcgc cataggctgg ccgccctcgc catcccggtc
    12061 gaccggctcg aggcggcgct cttgtccatc gatgctgccc ggaccggggc cgatctcggg
    12121 ctcgagcccg ccgcgtaccg ggccgccgtc gcccatgcgc acgagatccg cgaccgcttc
    12181 ggctttctcg acctcgccgc ccatgcgggg cggctagagg cgttcgccgc cggcgaaggg
    12241 taggggaatc gtgggcaccg tcctcgtgct cggcgccggg gtcatgggca gcgccttcac
    12301 cctgccgctg gccgatgccg gcacgaccgt ccggctggtc ggcacgcatc tcgacacggc
    12361 gctgatcgac ggcctgcgcg ccacgggcgt ccaccagcgg ctgaaggcgc cactgccggc
    12421 ggcggtcgag acctttcacc accacgagct ggccctggcc ctcgacgaca gcgtcgagct
    12481 ggtggtgctc ggggtcagct cggccggcat tgcctgggcg atcgaccggc tgcgcccgct
    12541 gctgtccgcg aagcggccgg tcctgctgct gaccaagggg ctcgctgccg acggaaggcg
    12601 gatcggcatc ctgccggagc cggtcgaggc ggcgctgggc tgcccgaccg gggcggtggg
    12661 cgggccctgc atcgccggcg agctggcggt tcgccgggcg acgagcgtcg tcatgagctt
    12721 cgccgacccg gcgctgatcc ggcaggtgct ggcgctgacc gccgccccct actaccacgc
    12781 ccgcgccagc gccgacctcg tcggcaccga ggtctgcgcc gccttcaaga acttcatggc
    12841 gctcggcatc ggcgccgccc ggggcatggc cgacgtggcc gggcccgctg acaacggcgc
    12901 cgccatgcac aacccggcgg ccgggctctt cacccaggcg gtgctcgagc tcggccggct
    12961 gaacgcgctc ctcggcggca cgcccgcgag cgtccacggc ctcgccggca cgggcgatct
    13021 ctacgtcacg gtccagggcg gccggaacag ccgcatgggc cggctcctcg gcgccggctg
    13081 ggtctattcc agggccaagg ccgagcgcat ggcggaggac acggtcgagg gcgccgagct
    13141 ggcgctcgcg gtcggcccga cgctcagggc gatgctcgac cgagatgacc tgcgagccga
    13201 cgacctgccc ttgaccctcg ccatcctcga tgccatcggc gaggatcggc ccctggtgct
    13261 gccctgggcc gccttccacc agcgcggccc gggtgccgcc tgaccggccc gaccccttgc
    13321 ccgagagcgc ttccttcgac cgcgtcgagc agctccgcac ccggatcgcc tggctctact
    13381 atgtcgaggg cctgacccag agcgacatcg ccgaccgcgt cggcctcagc cgggtccggg
    13441 tcaaccgcga gctcgcggtc tgccgcgagc gcggcatcgt gcagatccgc atcaattcca
    13501 ggctcgccac ctgcgtcgcc ctcgagcgcc ggctgatccg gcatttcggg ctgaaggacg
    13561 cggtggtcgt gccgacgccg gaggatccgg ccctcctgcc gggcatcatc ggcatggcgg
    13621 ccggccagta tgtctccgac cgtctggccg ccgggatgag catcggcgtc ggctgggggc
    13681 ggaccctgct cgggtcgctc aaggccatcg agcgacgcaa cgtccccggc ctctcggtcg
    13741 tctcgctgat gggcgggctg acccgggcat cggcgatcaa cacctacgag acggcgtccc
    13801 gcttcgccga tctcttcggc gccgactgct attatctcgc cgcgcccacc ttcacctcct
    13861 cgcccgagag ccgcgccacc ttcatcgagc aggcggcgat caaggacgtg ctcgaccggg
    13921 cccgcaaggt cgacctggcg ctggtcagcg tcggcgggct cggccctgat gccaccatcc
    13981 gccgcctcga catgctgagc gcggatgagc tggattcgct cgtcgaggcg ggtgcgctcg
    14041 gcgacagcct cggccatttc atcgacgccg ccggtcggct ggtggaccac ccgatcaacc
    14101 accgggtcat cggcctcgcc ccggccgagc tctcggacgt gccgaccgcc atcctcgcct
    14161 cgggcgggcc ggacaaggac cgggtcatcg gcgccgtgct cggggcgcgc ttcctcaacg
    14221 tcgcgatcat cgacgaggcg accgccgaac ggctgctggc ccgcgcctga gtccggcgct
    14281 atctccaggg tgatcgcccg agcggaaccg gagagccgcc ccatgccgtc cgatgtcgaa
    14341 cgcgagctcg tcgattggct gatcggcgcc gcgctggacc tcaccgacga gcagacgctg
    14401 ttcgccgggc tgtgcgaccg gctgagcctg gcgggggtcg ggatcacccg ggcctatctc
    14461 gcctccgagg tgctgcaccc gctgatcggg gcgcgcggct tcatgtggca gggcgaaggg
    14521 gccgtgcgcg aggattacgg ccacgacggc attggcgtgg tcgatccgaa ctggcggcgg
    14581 agccccttct tccacatgct ggaccggagc ctcgaccggc tgcacgtgcg gctcgacgcg
    14641 agcctcgagc cccggcgctt tccgctgctc gaccggctcg cggcgcaagg ctgcaccgac
    14701 tatctggcgc tcggccggcg catggtctcg accgctgcgc tcggcggcca ggacggcatc
    14761 ctcacctcgt ggacgaccat gcggccgggc ggcttcgagg ccggcgagac ggcgctcatc
    14821 gaacgctgca tggcgcccct ggccctcgcc ttcaagggct ttcgcgccgt cgacaccgcc
    14881 cgcaccctgg cctgcaccta tctcggccag gatgccggca gccgggtgct ggccggcgag
    14941 atccggcgcg gcgagccggt caccatcgag gcggtgctct ggtattcgga tctcgagggc
    15001 ttcacgcgga tcgccgacac cacgccgccc gaccggctga tggcgctgct caacgcctat
    15061 gcggaggtgc tggtcagcgt cgtcgatggc cgtggcggcg acgtgctcaa gttcatgggc
    15121 gacggcatcc tcgccctttt ccggggggac gacgcggccg cccgcgccct cgatgcggcg
    15181 atcgccgccc agcgcgcggt cggcgagctg aacgcgacgc gccgggcggc cggcgagccg
    15241 gtcacggagt tctatctcgg cctgcatcgc ggcgacgtgc tctacggcaa tatcggcagc
    15301 cgcgagcggc tcgacttcac ggtgatcggc ccggccgtca acgaggtggc gcggatcgag
    15361 gcgatgtgcc gctcgctcga ccggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnngtct tcttcctgct gcgggtcggc ggcatcacca tccgtgaccg ggtgcgctcg
    15541 acgctggagg tggaatccgg ctccaacgat ccgatggcga tcttcctcgt cgtcgtcctg
    15601 ctcgacctca tcctctcggg cgccggcctc gacacgctcg ggccgagcct gctccacgcc
    15661 ttcgccctgc agatgggcct cggcctcgcg gccggcctcg ccggcggctg gctcatcgtc
    15721 aagggcatca acctgatcga gctcgagccc ggcctctacc cgatcgccgt gctcggcggc
    15781 gccctcatgc tgttcgcgct caccgggctc ctcggcggca gcggcttcct cgcggtctat
    15841 gtggccggcc tcgtcgccgg caacatgcgg atccgcgccc ggccgcatct gcaccgcttc
    15901 caggacggcc tcacctggct cgcccagatc gtgatgttcc tgctgctcgg cctcttcgcc
    15961 acgccgtcga ccttcggcga gatagcgctc ccggtcatcg tcctcgccat cctgctggtg
    16021 gtcctgttcc ggccgctcgc cgtcaccctc ttgctgctgc cgttcggctt caggcgcaac
    16081 gaggtggcct tcatcagcgt ggtcggcctg cgcggcgccg tctcgatcct gctcgccatc
    16141 atgccgatca tggcccggct ggagcacggc cagacgctct tcaacgcggc cttcctcatc
    16201 gtcctcgtct cgctcctcac ccagggctgg agcatccgcc cgctggcccg ctggctcggt
    16261 gtcgtcgtgc cgccgcgcca gggcccggtc gacaaggtcg agctggagct gcccggcacg
    16321 gcgcaccacg agctcgtggt ctaccacatc accgagggca gcccggtcgc ccgcggccat
    16381 cgcctgccgc gctgggcccg gccgtcgctg gtggtgcggg gcggccgctc gctcagcgtg
    16441 catcaggcgg ggcacctcca gcccgacgac tatgtctaca tcttcacggc gccgagcttc
    16501 attcatctgc tcgaccgcct gttcgccagc cgcgccgcgc tcgaccccgc cgacatcgac
    16561 ttctacggcg agctgccgat cgtgccggac gcgccggtcg cggccctggc cgccacctat
    16621 ggcctggcga tcgagacgga tcccacgaca ccacgcatcg ccgactacct ggagcagcgc
    16681 ctcggcggcc atccggagcc cggcgaccgg ctgacgctcg gtgccgtcga cctcatcgtg
    16741 cgcagcgtcg acgacgcggg ccggatcgcc gagatcggcc tctcgttccg gcccatcgag
    16801 gaggcgacac gacagggcgg gacactggcc cgcatgcggc gtctcctggt cggccgcccg
    16861 caggccgccc cgatcgccga gccggaggaa gccccgaagg cgccctagcc tgctagagcg
    16921 gcgccaccgg ttgcggggcg gcgtcgtagc agccgagcac cgactggtcc atgtcgacca
    16981 gcgagcccgt catcatgccg ctctcgtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnngt gccggcgatc ttctcgcgcc gcatgatggt cgccgtgtcc tgcatcagga
    17161 agaagggtgc ccgcacattg atcgcgaaca tccgctcata gagctcgggg ctggtgtcga
    17221 ggatggtgcc gcgatcggtg atggcagcga cgttgacgag gcagtcgacg cggccgaagg
    17281 catcgtccgc cgccttcgtc accctgcgcg cctggtcgag gtcgccgaga tcggctgcga
    17341 cgaagacggc ttcggcgccg gccgcggcga ggtcggcccg cacccgctcg cccttttcgg
    17401 cgttgcggcc gcagatcacg acgcccctgg ccccgcgctc gacgaaaagg cgggccgtcg
    17461 cctcgcccag cccctgcgtg ccgccggtga cgatcgccac cttgccttcg aacccaccgc
    17521 tcatgcctgc ctccccgacc tgttcccgtc ctggccgcaa cctaccaagc ggcggtcgcc
    17581 caggccagcg ccgccgtcag gtgccggagg cggcgagccc gccggccgcc ggcgtcgccc
    17641 gccgctcgcc gtgccggagg aagaggcggc gcaggaagcc caggatcatc gcattgcgca
    17701 tgcgccgggc ctcggtcatg atcgcgctga catggacggg gtcgtaggct ccggcgagct
    17761 gccggtcggt ggtcatcgtc ttggctccag tcgcggcgct gtgcaggcgc ctcattgtcc
    17821 cttgttgcac tgcagcatac ttggccatcc aacgccccgg gggcaaccgg ccgttcgtgc
    17881 atggctcggt tgcgcggccg tttcaccctt tgatcatgaa gctggccagt cgggcacacc
    17941 cccgggtccg cgcggcccgg tgcatcgccc ggatccggcc ataggcacgg ccgtcgccca
    18001 cagcgatgcc ggcgaggccg agggcgtcgc acgtcgcggc gagcgccgga tcggcaatgg
    18061 ctgcggcgag ggcgcgtcgc agcgccgcca ccgtgcgccg gccggtcccg gcggcggtca
    18121 ccaagggcag ggcgggggcg ggcggcgtgg cggccagcac gcgcagcccg gccacggcgg
    18181 ccgggcgatg gcgggcgagc agggcgaagg tgacgcagtc gatcgccgcg cagtcggcgc
    18241 ggccctcgcg cacggccgcg agggacgccg cgtgcgcccc ggtctcgacg atgcccgaga
    18301 agaagggccg gccggcggcg aagggcacgg tcgccgccgc cagcgcgttg gccccggact
    18361 gcgaatgcgg cgcgttgacc gcggcccgcg agccctcgag ttcggcgagg ctgtcgtggg
    18421 gctcggcctc gcgcaccacg atccaggagc ggtaggcgcc gccttcgcag cccgaaatcg
    18481 cgtaggccgg tgtcgccacg aaccgcacgc ggccggccag cagcgtcgtg aagggcaggc
    18541 cgcaggcctg cgcgaggagg agcccgggat cggtccagag cccgatcagg tcgtcaccgc
    18601 gccacagccc tgccggcacg cccggcactc cggccgtccg gaggcgcagg gccaccgcct
    18661 gccagagccg gtcggtcgcc ggccgcagct ccggcaggtc gtacatcggc agcgcagcaa
    18721 tgcgctcccc ggcacggctc aattcgccac ctgctccaag ccggcgcggg cgcggcattt
    18781 cgtctgttca actaggggtt gtggacgccg tcgcatgtcg cgtgctagat aatcccgtcg
    18841 atttttcgac attctatttt gaggagcgtg gatgacgatc gatagcggga cggtcctgga
    18901 gatcggagag aacctcgtcg aggaaatcga cggcttcgag atggcgttcc cggcgggtgc
    18961 ctaccgggcc ctgccggtgc cgagcgccgc cctcgacagc gatcagggcg gggcgacgaa
    19021 cgccgacgcc gaccgctatc tgctcctgcc cgttggccgc gagcgcgtcc atccgatctc
    19081 cgccgagggc ctgggtcagc tcgtcgagct cgacagcgtg acggtcgccc gggccgattg
    19141 aggagcgccg gccagcttca gctcgcgatg gagagccccg ccgctgccgc cgcctcgctg
    19201 agcgccgcca tgccgatctc ggccatcttc aagggattgg ccttcttcgg gtcctgctcg
    19261 gcctcgatca ccacccagcc cgaatagccg atggtcttga gcgacttcat gaaggcgccg
    19321 aagtcgatcg agccgtcgcc gggcacggtg aagacgccct cgagcacgcc cttgaggaag
    19381 ctccagtcct ccgccttcag cttcttcagc acgtccttgc ggatgtcctt ggtgtggacg
    19441 tggttgatcc gcctgcccca gcgctcggtg gtggcgagca cgtcgccgcc ggcgaaggtc
    19501 aggtgcccgg tgtccaaaag cagccccacc ggcgcgtcgg tgctctccat gagcaggtcg
    19561 atctcctcct cggtctcgat caccgtgccc atgtggtggt ggaaggtcat cgggcagtgc
    19621 tcggcctgca gaaactcggc gaatttcgtc agcttctcgc cgtagcgcct gatctggtcc
    19681 ggcgtcagct tcggccgctg cgacaccggc gtgtcgatca ggccctgcac cgtgcccacc
    19741 gtctcggcat agaccatgac cgggcagccc atcgcctggt agagggcgag ctgctccttg
    19801 atccggctct tctcctcctc gaccgtcacc tcgagcagcc ggccggagaa ccagcccgag
    19861 accagctcga gctggtggtc cgccatgatc ggcttcagca gcttggggtc catcgggaac
    19921 ttgccgccct tctcgacccc gctgtagccg gcctgccgcg cctccttcag gcactgctcg
    19981 agggtgatgt cgccgccgag ctccggcagg tcgtcattgc tccagccgat gggggcgatg
    20041 ccgaggcgga tggccatccc tcagactccc acccgctgct tcttcttgcc gtccaggtgc
    20101 gctttcctgg cctcctgcac ggccggccgc tcgctcacct cgggcgtgcc gcactcccac
    20161 caggaattgt cgttgccggt ccagtcggtc gggtgcacgt cgatgtggat caccgtggt
//
LOCUS       DAOTFI010000067        20145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3224_length_20145_cov_6.375012, whole genome shotgun sequence.
ACCESSION   DAOTFI010000067 DAOTFI010000000
VERSION     DAOTFI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 20145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20145
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_20145_cov_6.375012"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="P5558_08665"
     CDS             <1..164
                     /locus_tag="P5558_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRY24419.1"
                     /translation="DPIQAKVMGLLPFIFVFLFATFPAGLVIYWTWNNLLSISQQWLI
                     MKRMGVPAS"
     gene            191..733
                     /gene="hpt"
                     /locus_tag="P5558_08670"
     CDS             191..733
                     /gene="hpt"
                     /locus_tag="P5558_08670"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRY24420.1"
                     /translation="MTTDERVVPLFTAAAIAARIDQLAASIAAEVPKDVTVVGLLKGS
                     FMFAADLIRALDRHGLSPYVEFLQVSSYGLERQSSGRVKVIGGMPDTVSGKDVLLIDD
                     IQDTGRTLTFTADLLRENGAGRIWSAALLDKPSRRVVPFEVDFIGFEIEDVFVVGYGI
                     DYAERYRHLPFIGRVERAGA"
     gene            726..1400
                     /gene="yihA"
                     /locus_tag="P5558_08675"
     CDS             726..1400
                     /gene="yihA"
                     /locus_tag="P5558_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRY24421.1"
                     /translation="MPEAPLVEDAAAREARLDEVARLLFARPPAFLRGVVALDQLPEV
                     AGPEVAIAGRSNVGKSSLINALTGRSDLARTSNTPGRTQELNYFELDGRLRLVDLPGY
                     GYAKAPKEKVAAWTELVFAFLRGRAPLRLVVLLIDARHGLKANDEEAMTLLGKAAVPF
                     VVVLTKGDLVGAAELAERRATILAALAKKPAAWPEVLITSARKREGLDALRRLLADLL
                     QDPARG"
     gene            1505..2437
                     /gene="argB"
                     /locus_tag="P5558_08680"
     CDS             1505..2437
                     /gene="argB"
                     /locus_tag="P5558_08680"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRY24422.1"
                     /translation="MPGTTDKTAIASTLIEALPYMRRHAGATFVIKYGGHAMGEEHLA
                     RDFARDVVLLKQVGINPIVVHGGGPQIKAMLDRLAIKSEFVDGLRVTDAATVEVVEMV
                     LAGRINKEIATAIQAAGGKAIGICGKDARLITAERLSRRRRDPGSAIEEVVDLGFVGE
                     PVAIDRTILDLCAQSEIIPVVAPIGAGPAGETYNINADTAAGAIAGALGARRLLMLTD
                     VKGVLDGDRNLLPWLTLHQVRALIADGTISGGMIPKVETCMHAVEAGVGAATILDGRV
                     SHALLLELFTEHGIGTQIRREPPRGAVGADPGNG"
     gene            2448..2864
                     /locus_tag="P5558_08685"
     CDS             2448..2864
                     /locus_tag="P5558_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24423.1"
                     /translation="MVAFLTLLLTGMLLGFMLTSAFVLPHLAMRYLAPATAGPFVREI
                     QPTCQRGAAAGALVAGLLVIGQLAGVLLVMVGATLLIQLFWLGPAIERYGRRGLAGER
                     SAMATAIHLHRANLAIGLFQFLTGVIAFALILREAG"
     gene            complement(2893..3543)
                     /locus_tag="P5558_08690"
     CDS             complement(2893..3543)
                     /locus_tag="P5558_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY24424.1"
                     /translation="MPERLLVIGNRNYSSWSLRPWLLLRQLGLPFTEERIALDQPDTK
                     ARILARSPAGRVPVLIEDELGVWESLAIIEHVADANNAVWPTEPATRAMARSISAEMH
                     GGFTALRNELPMNVRAAGRKVRTSPAADADIARIKAIWSSAREAHGAAGPWLLGAFSA
                     ADAMFAPVASRFRTYGIDLPDALAQWQRTVLECAAMQEWATAARAEAEVIEHEEVG"
     gene            3752..4294
                     /locus_tag="P5558_08695"
     CDS             3752..4294
                     /locus_tag="P5558_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HRY24425.1"
                     /translation="MFRRSALATVVLLASASMAMAGPQYVDETGYALSGYDPVAYFTL
                     EQPPLGEAQPHAVPGRADITADWNGATWAFATAENRDKFLADPEAYAPAYDGHCAYGV
                     AKGGKVPANPNLWRIVEGQLYVNITPTVQGFWTADVDGNIEAAETNWPGIEGDAASGK
                     SWTSMDDNAGTYETLAPIGG"
     gene            4320..4646
                     /locus_tag="P5558_08700"
     CDS             4320..4646
                     /locus_tag="P5558_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HRY24426.1"
                     /translation="MNDSTRTSTAMLAGALAAAIAGAGGFASPAHAANEKCYGISLAG
                     QNDCAAGPGTTCAGTSTVDYQGNAWKLVPEGTCQLYGTSTEEAKYELPGDREGSLEPL
                     ARDVPA"
     gene            4711..5553
                     /locus_tag="P5558_08705"
     CDS             4711..5553
                     /locus_tag="P5558_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HRY24427.1"
                     /translation="MIAATIPRASGLGLKSRHCAEILASRPRVGWFEVHPENYMGAGG
                     PPHRRLAAIRRDYPLSLHGVGLSIGGEGPLDAAHLARLRTLVERYEPGLVSEHLAWSS
                     HEGVYFNDLLPLPYTDGTLARVVEHVDRLQEGLGRPILLENPSSYLTFEESTLAEPAF
                     LGEIARRSGCGLLLDLNNVFVSSQNLGFTPESYIDSFPLDEVGEIHLGGHATEPDGLL
                     VDAHDRAVADPVWALLALVLQRTGVLPVLVEWDNDVPAWPVLHREAVLAQRLLDNCRH
                     ARAG"
     gene            5537..6295
                     /locus_tag="P5558_08710"
     CDS             5537..6295
                     /locus_tag="P5558_08710"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HRY24428.1"
                     /translation="MPAQAELAGALLAADRPAPAGLLPAGRFAVHRNNVVAGLVEALG
                     AAYPAVRSLVGERFFRAAATDFVRREPPRSPVLIDYGGGFAGFLGSFRPAAGLPYLAP
                     VARLERAWLLAYHAAEAAPLPIAALAQMPEAVLGRCRLVPHPSLRLVVSSFPVVALWA
                     ANTGRGPHEAVDLDRAETALVVRPEELVVVESLTPGMAAFVATLADDQPLGAAAEAAM
                     RADSGFELGPALGRLFALGAVVGLRQSSFEGDSR"
     gene            6292..6885
                     /locus_tag="P5558_08715"
     CDS             6292..6885
                     /locus_tag="P5558_08715"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRY24429.1"
                     /translation="MIDEVVELHQRFFRGLERLLDGWFLGLFARFVFAGVLLVYFLSS
                     AMTKIGAGFFGFLQLTPGAYIQIVPGAMEAAGYDPSAVAFWPYGLIVHLGTYAEFVLP
                     VLVVLGLFTRLAALGMIGFVVVQSYVDIAFHGADEATIGALFDRVSGSVIVDQRTLWL
                     MPLVYLMVKGAGVLSLDGLLARFFDEPLLGEPYAEWG"
     gene            complement(6895..7638)
                     /locus_tag="P5558_08720"
     CDS             complement(6895..7638)
                     /locus_tag="P5558_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24430.1"
                     /translation="MGLLSFHEPIRAAVIGASGGIGRAFTENLVADPAVRAVLATSRQ
                     GAVPEDERIQALRLDLEDEASIEAAAASANDRLGGLDLVVVATGILHGQGISPEKSYR
                     QLDPAAMARVLALNTIGPALVAKHFLPLLPREGRCILAALSARVGSIGDNALGGWHSY
                     RASKAALNMLIRNFAIEQARRNDQSIIVGLHPGTVASDLSAPFQGGVPAGRLFTPEHA
                     ASRLLATLDALTPEDSGGIFAYDGERLPY"
     gene            complement(7696..8610)
                     /locus_tag="P5558_08725"
     CDS             complement(7696..8610)
                     /locus_tag="P5558_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY24431.1"
                     /translation="MAFGRFLAAAAALVVAAGGSFWYLTIPERYDPLDLPAHEPDLAN
                     GELLFWAGGCTSCHAAPGAKGEARLLLVGGVALETPFGRFNVPNITPDPDSGIGGWTM
                     AEFVTAMRYGTSPAGRHYYPAFPYTSYARMTVTDVMDLKAWLDTLPAEPSIVPDHELA
                     FPYNFRRGLGLWKLLNLDPAPVVDVGADPELERGRYLTEGPGHCGECHTPRDVTGGLE
                     LKHWLAGAPAPDGKGRIPNITSGPDGLADWSTGDIAYALESGFKPDYDTLGSTMAEVQ
                     ANLAKLPASDREAIAAYLKATPPLLEGS"
     gene            complement(8610..9116)
                     /locus_tag="P5558_08730"
     CDS             complement(8610..9116)
                     /locus_tag="P5558_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY24432.1"
                     /translation="MSRHPLSANVFSSSGIREGYDMSPRLIAAIAALGITAIAGFASA
                     DPIETRQTIMKSMGAAGKVSGEMIKGNLPFNQDVANLALATFAAASLSYREFFPEGSQ
                     AGHDTRAKDTIWTDRAGFIAANEKFAEAATAAVAAKPADLDAFKAAFAPIGENCQSCH
                     ETYRTPSS"
     gene            complement(9136..11196)
                     /locus_tag="P5558_08735"
     CDS             complement(9136..11196)
                     /locus_tag="P5558_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24433.1"
                     /translation="MTAASLVLQPLIPLWLWATLAIVALLLFAFGLWRRARGTLWRGL
                     LAAFLLLALAGPELQREERQALDDIVMLVEDRSPSQRIANRPEATSEAADGLAERLAA
                     LPGIDLRRVTLEGSGRRGTDLFATLRERLGEVDQQRLAGIAVVTDGRVHDAPPTPDWG
                     VDAPFHVLLTGRPDEFDRRIVVEQAPTFGVVGEPVPVTLRIATTPEAAASAEPLPLTV
                     ERNGEPFTELVVPPGEPVSLDLQLENAGATVFEFQIPVADGELTALNNRQAITVNGIR
                     DRLRVLLVSGQPYPGLRVWRNLLKSDPAVDLVHFTILRPPEKQDGTPIRELALIAFPS
                     RELFEVKLGEFDLIIFDRYSRRGLLPLVYLDNVARYVEEGGALLDAAGPEFADPQSLY
                     RTPLSRVLPGRPSGEVYERAFRPEVTELGGLHPVTSGLDAQNDTEEWGRWFRQIDVET
                     DDAQVLMTGVADRPLLVLERVGQGRVAQLLSDHAWLWARGFDGGGPQATLLRRLVHWL
                     MREPELEEEALRARAVGDGLLIERQSLHAEPVEVEVTAPSGATTTVLLEPTSEGLATA
                     TVEADEDGLYRVGDGKLLAFATPRPIAPVELADMTATEEVLAPFAAATGGAVHWLEER
                     GQPDIRRVEAERRASGAGWIGFERNRAYAVLSLRQTPLVPAWLAALVLLAAAAAAWWR
                     EGRG"
     gene            complement(11193..14003)
                     /locus_tag="P5558_08740"
     CDS             complement(11193..14003)
                     /locus_tag="P5558_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRY24434.1"
                     /translation="MLALGPIAFLQPWILAGLLALPLIWLLLRLRPPAPRRVAFPPLA
                     LLLRLVAPEETPARTPWWLLLLRLAIAALVLLALAGPVLDPAPVIGGRGPVLLVVDDG
                     WAAAPNWQRRVGLARGLLEQAERDSRPVVVLKTAPDGRSTPLEPQPARQVLEGLAGWQ
                     PHPWPVDHAAALARLEASALSEARPVWLSDGLIDRDGEAEAARALATALGAIGELSIH
                     ADPPAERALLLSPDDLARDLQSVSVLRPAADAPLPPELFAIGPAGEVLGRFPITAPGG
                     DDADPLRATVELDLPLDVRNRIARLTLEGNHGIGGVLLFDERWRRRTVGIAGPPGAAA
                     AQPLLAEPYFVERALAPFAELRTGRITELVEEPLSLLVITDRGHLDPAEAAALEAWVE
                     TGGVVLRFAGPRLAGLDEASQPVGEVEHDPLLPVRLRSGDRQLGGALSWSEPLPLAPF
                     PADSLFAGLDVSEEALVYRQVLAEPGPDLAEATLASLADGTPIVTGRKAGDGYILLVH
                     TTANTSWTSLPLSGLFVEMLRRVVDLGVGAGERLDGLVRADRVLAADGSLVEPPADLQ
                     PIEAAALADLPVGPASPAGLYAPVDAGTTPGDMAEDERGRVARNLQDSLDRLESLDVA
                     TLGEGALPYAQGTERDLLPPLLTLATILALVDLALSFALRGLLPRLRPAASAAALLAI
                     CLLPGLANAQESIDEARIAELAAITRLGYVETGIADLDERSRAGLFGLGRVLEQRTSI
                     ETGDPVAVDPAVDELAVFPLLYWPVPPEHPALDTAAIDRIEDYLRQGGMVLFDTGDAA
                     RLLPGQNIVGPGEQRLAELLRDLDLPPLEPIPDDHVLTRSFYLMQDLPGRFAGRPVWV
                     DRMPQSVNDGVSSVVIGAHDWAGAWAEDEYGRTLYPVVPGGERQREMARRFGVNIVMY
                     ALTGNYKTDQVHIPALLERLGQ"
     gene            complement(14006..14920)
                     /locus_tag="P5558_08745"
     CDS             complement(14006..14920)
                     /locus_tag="P5558_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY24435.1"
                     /translation="MVSEPTAALEAGHVAALRGEAERLGGRLPGLLVEAERVATTVSQ
                     GVHGRRRPGLGETFWQFRQYQPGDAAGSIDWRQSARSRHLFVRQQEWEAAESVWLWAD
                     QSPSMRYRSKPALATKRERALVLLLALAGLLVRGGERIALLGTGERPQGGRYGFNLVV
                     ERLLRGAAGAGFRLPPQPLPRHARLVLVSDFLEPADHLAERVRTLGALGAEVSLMMLA
                     DPAEETLDFQGRVLFEGLEGEGRALIGNVEAIRSDYRDLYRAHHEHLADLARRHAWQF
                     ARHRTDRPTETGLLTLYQMLAPRAAGRH"
     gene            complement(14929..15855)
                     /locus_tag="P5558_08750"
     CDS             complement(14929..15855)
                     /locus_tag="P5558_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY24436.1"
                     /translation="MKAEIGRVIFGQENVVEEVLIALLAGGHALLVGVPGLAKTRLAE
                     TLGTVLGLTEKRIQFTPDLMPADIIGSEVLEEDSGGRQHFRFIEGPIFTQLLMADEIN
                     RASPRTQAALLQAMQERKVTVAGHDHRLPAPFHVLATQNPIEQEGTYPLPEAQLDRFI
                     MQIDVDYPSVAAERQMMIATTSGNEARPQAVIDGPELMAMQALVRRLPVGESLVEAIL
                     QLVRAGRPEASPVDGIAEQVAWGPGPRASQALMLVTRARALLGGRLAPSLEDLQALAG
                     PVLRHRMAVTFAARADGIGVEDVIERLVRHVG"
     gene            16202..16666
                     /locus_tag="P5558_08755"
     CDS             16202..16666
                     /locus_tag="P5558_08755"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HRY24437.1"
                     /translation="MAIDAEGHWHHEGRRIARPGMARLFSTALRRAADGSYWLVTPFE
                     AGRVEVEDVPFVVVELKVEGEGKGRTIHLRTGLDEWLRLDADHPLVMRPPPGGGVPAP
                     YVTVRPGLEARLARAVYYHLVELAEPAADDEDLLGVWSAGRFVPLGSVESGA"
     gene            16669..17280
                     /locus_tag="P5558_08760"
     CDS             16669..17280
                     /locus_tag="P5558_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRY24438.1"
                     /translation="MSASSWTPYRLAERLRDALAEEGPRHIGGDEVARPLEPARLRPA
                     AVLIALVERPGGPSLVLTRRAAHLKAHAGQTSLPGGRMEPEDVSAVATALRESEEEIG
                     LPPDRVAVLGGLRSYDTITGFRIHPVVGWVADPPAWRADPAEVAEIFEVPLAFVLDPA
                     NHTRESYLRDGQRRYFYVLPHADHYIWGATAGILVNFARLLRA"
     gene            17303..17620
                     /locus_tag="P5558_08765"
     CDS             17303..17620
                     /locus_tag="P5558_08765"
                     /inference="COORDINATES: protein motif:HMM:NF040327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HRY24439.1"
                     /translation="MLRIVISYLLPFILPFAAFFLYRLLMTSGQPLLRRTPWFVLSVI
                     GMALVAGTLVSFALFGGEAQEGVYVPPSFEDGRVVPGHVRPPLAEVPDEGAPADGDAS
                     EPQ"
     gene            17617..18834
                     /locus_tag="P5558_08770"
     CDS             17617..18834
                     /locus_tag="P5558_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRY24440.1"
                     /translation="MIEERPLAHAAWLRARASRRVLDALEGDGTEVRFVGGCVRDTLL
                     DPTLDIVDLDLATVATPERNLELLADAGIKTFPTGLAHGTITAHTGSRSFEITTLRRD
                     VATDGRRAVVTYTDCFTADAARRDLTINTLSCDRFGRVFDTFGGRADLAAGIVRFVGE
                     PTARIREDYLRILRFFRFQARFGRVPPDTATLAALAAERAGLDLLSGERVRSEVLRLL
                     MAPGVLAALELMRSCGVWRQVFAGEPALEDLRRLLDLAPDATAILRLACLLRDRSDPA
                     AVAARLRLSNAERERLAQAMSGELPGIDAGPVTLRRLAYRLGTEGADDRLVVAAARTA
                     VAPDTVAEARRIVRDFGRRSLPVGGKDLLARGMAPGPGLGARLRELEDAWIASDFKLD
                     REMLLKQLGGGDH"
     gene            18831..19790
                     /locus_tag="P5558_08775"
     CDS             18831..19790
                     /locus_tag="P5558_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY24441.1"
                     /translation="MSGRFAVVGAGVAGVACARALADAGWRVAIHEREPRIGGRLAQC
                     HQEGAVFDYGGQFVRPRDPVLKALLEAGVAAGVAARWPAADRDGVAAYVGRPTMAAPL
                     EALLHGIETRTGCQIVRLSRDGGSWWLEDEGGARHGPFDRVGLALPPVIAGQLLGTVE
                     APPEALVAATAAAALAPCWALLLAFDPPLDLHGFDARVIDEGPLAWIARNTTKRGRQG
                     RDSWTAHATQDWSAAHLELSPEAVLEQLVPAFRAVTGTADVPFHAGAHCWRQAQVTRA
                     LGVAAVHDPGAGLGLCGDWCLSRNVEAAFLSGRALAGLLGEGG"
     gene            19957..>20145
                     /gene="rpsB"
                     /locus_tag="P5558_08780"
     CDS             19957..>20145
                     /gene="rpsB"
                     /locus_tag="P5558_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRY24442.1"
                     /translation="MAFPTFTMRQLLEAGVHFGHQTRRWNPRMEPYIFGVRNGVHILD
                     LTQTVPLMHRALQAVRDVV"
BASE COUNT         2839 a         6681 c         7555 g         3070 t
ORIGIN      
        1 ccgatccgat ccaggccaag gtgatggggc tcctgccgtt catcttcgtc ttcctcttcg
       61 cgaccttccc ggccggcctc gtcatctact ggacctggaa caatcttctc tcgatcagcc
      121 agcagtggct gatcatgaag cggatgggcg tcccggcgag ctgacggcgc cggcaggagg
      181 aggtgttccg atgaccacgg acgaacgggt ggtgccgctg ttcacggccg cggcgatcgc
      241 ggcgcggatc gaccagctcg cggcgtcgat cgcggcggag gtgccgaagg acgtcaccgt
      301 ggtcggcctg ctcaagggca gcttcatgtt cgccgccgac ctgatccgtg ctctcgatcg
      361 gcacgggctc agcccctatg tcgagttcct gcaggtcagc tcctacgggc tcgagcggca
      421 gagcagcggg cgggtcaagg tgatcggcgg catgccggac acggtgagcg gcaaggacgt
      481 gctgctgatc gacgacattc aggataccgg ccgcacgctg accttcaccg ccgatcttct
      541 gcgcgagaac ggtgccgggc ggatctggag cgcggcgctg ctcgacaagc cgtcgcgtcg
      601 ggtcgtgcct ttcgaggtgg acttcatcgg cttcgagatc gaggatgtct tcgtcgtcgg
      661 ctacggcatc gactatgcgg agcgctaccg gcatctgccg ttcatcggcc gggtggagcg
      721 tgccggtgcc tgaggcgcct ttggtcgagg acgcggcggc gagggaggcc cggctcgacg
      781 aggtggcgcg gctgctcttc gcccggccgc cggccttcct gcgcggtgtc gtggcgctcg
      841 accagctgcc cgaggtggcc ggacccgagg tcgccatcgc cggccgctcc aatgtcggca
      901 agtccagcct catcaatgcg ctcaccggcc gctccgatct ggcccgcacc tccaacaccc
      961 ccgggcgcac ccaggagctc aactatttcg agctcgacgg tcggctccgg ctcgtcgacc
     1021 tgccgggcta cggctatgcc aaggcgccga aggagaaggt ggccgcctgg accgagctcg
     1081 tcttcgcctt cctgcgtggc cgggccccgc ttcgcctcgt cgtcctcttg atcgacgccc
     1141 ggcacgggct gaaggccaat gacgaggagg ccatgaccct gctcggcaag gccgcggtgc
     1201 cgttcgtcgt cgtgctcacc aagggcgatc tggtcggcgc cgccgagctc gccgagcgtc
     1261 gggcaactat tctcgcggcc ctcgcgaaga agccggcggc ctggcccgag gtgctgatca
     1321 ccagtgccag gaagcgggaa gggctcgacg cgctccgccg cctgctcgcc gatttgctgc
     1381 aggacccggc caggggctga gcgcccaccc ggccgtcccg gccgagggcc gacgtggcgt
     1441 gctgaccccg ggccgggcgc ctgctagaac cgggccgaca gcgttcccga ggaagcgaac
     1501 cgccatgccc ggcacgaccg acaagaccgc catcgccagc acgctcatcg aggcgctgcc
     1561 ctacatgcgg cgccatgccg gcgccacctt cgtcatcaag tatggcggcc atgccatggg
     1621 cgaggagcat ctggcccgcg acttcgcccg cgacgtggtg ctgctcaagc aggtcggcat
     1681 caacccgatc gtggtgcatg gcggggggcc gcagatcaag gcgatgctgg accgcctcgc
     1741 catcaagagc gagttcgtcg acgggctcag ggtgacggat gccgccacgg tcgaggtggt
     1801 cgagatggtc ctcgccgggc ggatcaacaa ggagatcgcg acggccatcc aggcggccgg
     1861 cggcaaggcc atcggcatct gcggcaagga tgcgcggctg atcaccgccg agcggctgag
     1921 ccgcaggcgg cgcgacccgg gaagcgccat cgaggaggtc gtcgatctcg gcttcgtcgg
     1981 cgagccggtg gccatcgacc ggacgatcct cgatctctgc gcgcaatccg agatcattcc
     2041 ggtggtggcc ccgatcggcg cggggccagc gggcgagacc tacaacatca atgccgacac
     2101 ggcggcgggt gcgatcgccg gggccctggg tgcgcgccgc ctcctgatgc tcacggacgt
     2161 caagggtgtg ctcgatggcg accgcaatct cctgccgtgg ctgacactcc accaggtgcg
     2221 ggcgctgatt gccgacggca cgatctctgg cggcatgatt cccaaggtcg agacctgcat
     2281 gcatgcagtc gaggcgggcg taggtgccgc caccattctc gacggcaggg tgtcgcacgc
     2341 gctcctgctc gagcttttca ccgagcacgg catcggcacc cagatccgcc gcgagccgcc
     2401 caggggtgcc gtgggcgccg atccggggaa cgggtgacgg gaggctgatg gtcgcttttc
     2461 tgacgctcct gctcaccggc atgctgctcg gcttcatgct gacctcggcg ttcgtcctgc
     2521 cgcacctcgc gatgcgctat ctggcgccgg ccacggctgg acctttcgtg cgcgagatcc
     2581 agccgacctg ccagcgcggc gccgcggcag gagcgctggt cgccgggctg ctggtcatcg
     2641 gccagctcgc cggcgtgctg ctggtgatgg tcggtgccac cctgctcatc cagctcttct
     2701 ggctcggccc cgccatcgag cgttacggcc gccgcggcct cgccggcgag cgatcggcca
     2761 tggcgaccgc gatccacctg catcgggcca atctcgcgat cgggctcttc cagttcctga
     2821 ccggcgtcat cgccttcgcg ctcatcctgc gggaagccgg ctgagcgtcg gtccggcggg
     2881 agtcggcccc gctcagccga cctcctcgtg ctcgatgacc tcggcctcgg cccttgccgc
     2941 tgtggcccat tcctgcatgg cggcgcattc cagcacggtc cgctgccatt gggcgagggc
     3001 gtccggcagg tcgatgccgt aggtgcgaaa gcgtgaggcg acgggcgcga acatggcatc
     3061 cgccgcagag aaggcgccga ggagccaggg gccggcggcg ccgtgcgctt cacgggcgct
     3121 ggaccagatg gccttgatgc gggcgatgtc ggcatcggcg gccgggctgg tgcggacctt
     3181 gcggcccgcc gcacggacat tcatcggcag ctcgttgcgg agtgcggtga agccaccgtg
     3241 catctcggcc gagatcgagc gggccatggc gcgggtggcg ggctcggtgg gccagacagc
     3301 gttgttggca tcggccacgt gctcgatgat ggcgagtgat tcccatacgc cgagctcgtc
     3361 ctcgatcagc acgggcaccc ggccggccgg ggagcgggcc aggatgcggg ctttggtgtc
     3421 gggctggtcg agggcgatcc gctcctcggt gaagggcagg ccgagctggc gaagcaggag
     3481 ccaggggcgg agcgaccagg aggagtagtt cctgttgccg atgacgagca gcctctcggg
     3541 catggcggac ggtcccggtt gttggacggc catcgagccc agcgatgccg ggtttggcaa
     3601 acgctctttc gttatcgggc gcaacagcga tgcttatcgc cccggacttc tcacgagcgg
     3661 cccacgcgtt cgttacgaga cgcgatttgc cgttcccgca ttcggccgcc taggttccgg
     3721 catcggacca gcagcgaagg gggatgtgat catgttccgc aggagtgcgc tcgccacggt
     3781 cgtccttctg gccagcgcca gcatggccat ggccgggccc caatacgtgg acgagaccgg
     3841 ctatgcgttg agcggctacg atcccgtcgc ctacttcacg ctggagcagc cgccgctcgg
     3901 cgaggcccag ccgcatgccg tgcccgggcg cgccgacatc acggccgact ggaacggtgc
     3961 tacctgggcc tttgccacgg ccgagaaccg ggacaagttc cttgccgacc ccgaagccta
     4021 cgcgccggcc tatgacgggc actgcgccta tggcgtggcg aaaggtggca aggtgccggc
     4081 caatccgaac ctctggcgca tcgtcgaggg ccagctctac gtaaacatca cgcccacggt
     4141 gcagggcttc tggacggccg atgtggacgg caacatcgag gccgccgaaa ccaactggcc
     4201 agggatcgag ggcgatgccg cctcgggcaa gtcctggacc agcatggacg acaacgccgg
     4261 cacctacgag acgctcgccc cgatcggcgg gtgatcggga acgccaagga gtattgcaga
     4321 tgaacgacag caccaggact tcaaccgcca tgctggccgg cgccctcgcg gcggcaatcg
     4381 ccggggcggg cggcttcgcc agcccggcgc acgcggcgaa cgagaagtgt tacggcattt
     4441 cgctggccgg gcagaacgac tgcgccgccg gtccgggcac gacctgtgcc ggaacgtcga
     4501 cggtcgacta ccagggcaat gcctggaagc tggtgccgga gggcacctgc cagctctacg
     4561 gcacgtcgac ggaagaggcc aagtacgagc tgccgggcga ccgcgagggt tctctcgagc
     4621 cgctggcccg cgacgttccg gcctgaacgg gatcggacct gcagcgggcg cggtgcttcg
     4681 gcaccgcccc gctcgggtcc atgtgctgtg atgattgccg caaccattcc aagggcttcc
     4741 ggcctcggcc tgaagtcccg acattgtgcc gagatcctgg cgagccggcc ccgggtcggc
     4801 tggttcgagg tgcatcccga gaactacatg ggcgccggtg gaccgccgca tcgccggctc
     4861 gctgccatcc gccgggacta tcccctgtcg ctgcacggtg tcggcctctc gatcggcggc
     4921 gaggggccgc tggacgcggc gcatctggcg cgtcttcgga cgctggtgga gcgctacgag
     4981 ccggggctgg tctccgagca tctggcctgg tcgtcgcacg agggcgtcta tttcaacgac
     5041 ctcctgccct tgccctacac ggatggaacg ctggcacggg tggtcgagca tgtcgaccgt
     5101 ctgcaggaag ggctcggccg accgattctc ctcgagaacc cgtcctccta tctcaccttc
     5161 gaggagagca cgctggcgga gcctgcattt ctcggcgaga tcgcccggcg gagcggctgc
     5221 ggcctcctgc tcgacctcaa caatgtcttt gtttcatccc agaatctggg gtttacaccc
     5281 gaatcctaca tcgattcctt ccccctcgac gaggtcggcg agatccatct gggcgggcat
     5341 gccacagagc cggatgggct gctcgtcgac gcccatgacc gggccgtggc ggatccggtc
     5401 tgggcgcttc tggcgctcgt gctgcaacgg accggcgttt tgccggtgct ggtcgaatgg
     5461 gacaacgacg tgccggcgtg gccggtgctg caccgggagg cggtgctggc ccagcgactc
     5521 ctcgacaatt gtcgtcatgc ccgcgcaggc tgagctggcc ggcgcactcc tcgccgccga
     5581 ccggccggcg ccggcgggtc tgcttccggc cggacggttc gctgtccatc gcaacaacgt
     5641 ggtggccggc ctggtcgagg cgctcggtgc cgcctatccg gcggtgagga gcctcgtcgg
     5701 cgagaggttc ttcagggcgg ccgccaccga tttcgtgcgg cgggagccgc cgcgctcgcc
     5761 tgtgctgatc gactacggcg gcggctttgc gggctttctg gggagtttcc gaccggcggc
     5821 agggcttcct tacctggcac ccgtggcacg gctcgagcgg gcctggcttt tggcctatca
     5881 cgcagccgag gcggcgcccc tgccgatcgc ggcgctggca cagatgccgg aggcggtgct
     5941 cgggcgttgc cggctcgtgc cgcacccgag cctgcggctc gtcgtctcgt cctttccggt
     6001 cgtcgccctc tgggccgcca acaccggtcg cgggccgcac gaggcggtcg atctcgaccg
     6061 ggccgagacg gcgctggtgg tgcggcccga ggagcttgtc gtggtggagt cgctgacacc
     6121 gggcatggcg gcgttcgttg cgaccctggc cgatgaccag ccgctcggag cggccgccga
     6181 ggcggcgatg cgggccgatt ccgggttcga gctcgggccg gcactcggcc ggctctttgc
     6241 cctgggtgcc gtcgtcgggc tgcgccaatc ctcttttgaa ggagactctc gatgatcgac
     6301 gaggtggtcg agctgcacca gcgcttcttc cgcggcctgg agcggctgct cgatggttgg
     6361 ttcctcgggc ttttcgcccg cttcgtcttc gccggggtgc tcctggtcta tttcctgagc
     6421 tcggccatga ccaagatcgg ggcaggtttc ttcggcttct tgcagcttac accgggcgcc
     6481 tacatccaga tcgtgccggg ggcgatggag gcggcgggct acgatccgtc ggccgtggcc
     6541 ttctggccat acgggctcat cgtccatctc ggcacctatg cggagttcgt cctgccggtg
     6601 ctggtcgtgc tcggcctctt cacccggctc gcagcactcg gcatgatcgg cttcgtcgtt
     6661 gtccagagct atgtggacat tgccttccac ggcgccgacg aggcgaccat cggcgccctc
     6721 ttcgaccgtg tctcgggctc ggtgatcgtc gaccagcgca cgctctggtt gatgccgctg
     6781 gtctatctca tggtgaaggg ggccggggtc ctgtcgctcg acgggctgct ggcgcggttc
     6841 ttcgatgagc ctttgctcgg ggagccttat gccgagtggg gctgagcgcg gcggtcaata
     6901 gggcaggcgc tcgccatcat aggcgaagat gccgcccgag tcctccggtg tgagcgcgtc
     6961 gagcgtggcg aggaggcggc tcgcggcatg ctcgggcgtg aagagccggc ccgcgggaac
     7021 gccgccctgg aaaggggcgg agaggtcgct ggcaaccgtg ccgggatgca ggccgacgat
     7081 gatgctctgg tcgttgcggc gggcctgctc gatggcgaag ttcctgatca gcatgttgag
     7141 ggcggccttg gacgcccggt agctgtgcca gccgccgagt gcgttgtcgc cgatcgagcc
     7201 cacgcgggcg gagagggcgg ccaggatgca gcgtccctcg cgcggcagca gcggcaggaa
     7261 atgcttggcg acgagcgccg ggccgatggt gttgagcgcc aggacccggg ccatggcggc
     7321 ggggtcgagc tggcgatagg atttctccgg gctgatgccc tggccgtgca ggatgccggt
     7381 ggcgacgacg acgaggtcga ggccgccgag ccggtcgttc gcgctggcgg ccgccgcctc
     7441 gatgctcgcc tcgtcctcga ggtcgaggcg aagcgcctgg atgcgctcgt cctcaggcac
     7501 cgcaccttgc cgcgaggtgg cgaggacagc ccgcaccgcg gggtcggcca cgaggttctc
     7561 ggtgaaggcg cggccgatgc cgccggaggc gccgatcacg gcggcgcgga ttggctcgtg
     7621 gaagctcagt agacccaagg gaatgccttt cggaggttct ctcctcgata cgtccgaaag
     7681 ggccgaatgg ttcgctcagc tgccctcgag caaaggtggc gtggccttca ggtacgcggc
     7741 gatggcttcg cggtcgctgg ccggcagttt ggcgagattg gcctggacct cggccatggt
     7801 gctgccgagc gtatcgtagt cgggcttgaa gccgctctcg agggcgtagg cgatatcgcc
     7861 ggtcgaccag tcggcgaggc cgtcaggacc gctggtgatg ttgggaatgc ggcccttgcc
     7921 gtccggggcg ggcgcaccgg cgagccagtg cttcagctcg agcccgccgg tgacgtcgcg
     7981 gggtgtgtgg cattcgccgc aatggcctgg accttcggtt agatagcggc cgcgttcgag
     8041 ctcggggtcg gcgccgacgt cgacgactgg ggccgggtcg aggttgagca gcttccacaa
     8101 gccgaggccg cgacggaaat tgtagggaaa ggcgagctcg tgatcaggaa cgatgctcgg
     8161 ttcggccggc agcgtgtcga gccaggcctt gaggtccatc acgtcggtga ccgtcatccg
     8221 ggcgtaggag gtgtagggaa aggccggata gtagtgtcgg cctgcgggcg aagtgccgta
     8281 gcgcatggcg gtgacgaact ccgccatggt ccagccgccg atgccgctat cggggtcggg
     8341 ggtgatgttc ggcacgttga agcggccgaa aggtgtctcg agggcgacgc cgccgaccag
     8401 gaggaggcgg gcctcaccct tggcccccgg ggcggcgtgg caggaggtgc agccgccggc
     8461 ccagaagagg agctcgccat tggcgaggtc gggctcgtgg gccggcaggt cgagcggatc
     8521 gtagcgctcg ggtatcgtga gataccagaa gctgccgccg gccgcgacga ccagcgccgc
     8581 ggcggcggca aggaaacggc caaaggccat cagctgctgg gcgtgcggta ggtctcgtgg
     8641 caggactggc agttctcgcc gatcggggcg aaggcggcct tgaacgcgtc gaggtcggct
     8701 ggcttggcgg cgacggcggc ggtcgccgcc tcggcgaact tctcgttggc ggcgatgaag
     8761 cccgcacggt cggtccagat ggtgtccttg gcgcgggtgt catggccggc ctggctgccc
     8821 tcgggaaaaa actcacgata gctcagggag gcggcggcga aggtggcgag cgccaggttg
     8881 gcgacgtcct gattaaaggg caggttgccc ttgatcatct cacccgaaac cttgccggcg
     8941 gcgcccatcg atttcatgat cgtctggcga gtctcgatag ggtcggcgct ggcgaagccg
     9001 gcgatggcgg tgatgccgag cgccgcgatg gcggcgataa gcctgggcga catgtcgtat
     9061 ccctcccgta tgcctgaaga cgaaaataca ttggccgaca ggggatggcg agacaatcag
     9121 gtcacgcaca gttgatcagc cgcgaccctc gcgccaccag gcggcggctg cggcggcgag
     9181 cagcacgagg gcggcgagcc aggcggggac cagcggtgtc tggcgcaggc tgagcacggc
     9241 gtaggcgcgg ttgcgctcga agccgatcca gcccgcacca ctggcgcgcc gctcggcttc
     9301 gacccggcgg atgtcgggct ggccccgttc ctcgagccag tgcacggccc cgccagtggc
     9361 ggcggcgaag ggggcgagga cctcctcggt cgcggtcatg tcggcgagtt cgaccggggc
     9421 gatcgggcgg ggtgtggcga aggccaggag cttgccgtcg ccgacccggt agagcccgtc
     9481 ctcgtcggcc tcgaccgtgg ccgtggcgag gccctccgag gtcggctcga gcagcacggt
     9541 ggtggtggcg ccggaggggg cggtgacctc gacctcgacc ggctcggcgt gcagcgactg
     9601 gcgctcgatc aggaggccgt cgccgaccgc ccgggcgcgc agcgcctcct cctcgagctc
     9661 cggctcgcgc atcagccagt gaacgaggcg gcggagcagg gtggcctgcg ggccgccgcc
     9721 gtcgaagccg cgtgcccaga gccaggcatg gtcggagagg agctgcgcca cgcggccctg
     9781 gccgacccgc tcgagcacca ggagcggacg gtcggcgacg ccggtcatca gcacctgggc
     9841 gtcgtccgtc tcgacgtcga tctgacgaaa ccagcggccc cattcctcgg tgtcgttctg
     9901 ggcatcgagg ccggaggtca ccggatggag gccgccgagc tcggtcactt ccgggcggaa
     9961 ggcgcgctcg tagacctcgc ccgacgggcg gcccggcagg acgcgcgaca ggggtgtccg
    10021 gtagaggctt tgcgggtcgg cgaactcggg accagcggca tcgagcaggg cgccgccctc
    10081 ctcgacatag cgggcgacgt tgtcgagata gacgagcggc agcaggccgc gcctcgaata
    10141 gcggtcgaag atgatgaggt cgaactcgcc gagcttgacc tcgaagagct cgcgggacgg
    10201 gaaggcgatc agggccagct cgcggatcgg cgtgccgtcc tgcttctcgg gcgggcgcag
    10261 gatggtgaag tggacgaggt cgacagccgg atcggacttg agcaggttgc gccagacgcg
    10321 caggcccgga taaggctggc cggagaccag gagcacacgc agccgatcgc ggatgccgtt
    10381 gacggtgatc gcctggcggt tgttgagggc ggtgagctcg ccgtcggcga cgggtatctg
    10441 gaactcgaag accgtggccc cggcattctc gagctgcagg tcgagggaga ccggctcgcc
    10501 tggcggcacc acgagctcgg tgaagggctc gccattgcgc tcgacggtga gcggcaaagg
    10561 ctcggccgag gcggcggcct ccggggtcgt ggcgatgcgc agcgtgaccg gcacgggctc
    10621 gccgaccacg ccgaaggtcg gcgcctgctc caccacgatc cgccggtcga actcgtccgg
    10681 ccggccggtc aggagcacgt ggaagggggc gtcgacgccc cagtccgggg tgggcggggc
    10741 gtcgtggacg cgaccgtcgg tgacgaccgc gatgccggcg aggcgctgct ggtcgacctc
    10801 gcccagacgc tcgcgcagcg tggcgaagag gtcggtgccg cgccggccgc tgccttcgag
    10861 cgtaacgcgc ctgaggtcga tgccgggcag tgctgcgagc cgctcggcca ggccgtccgc
    10921 cgcctcgctc gtcgcttccg ggcggttcgc gatgcgctgg ctcgggctcc ggtcctcgac
    10981 cagcatgacg atgtcgtcga gcgcctggcg ttcctcgcgc tgcagctccg gcccggcgag
    11041 ggccagcagg agaaaggcgg cgaggaggcc gcgccagagc gtgccgcgag cccgccgcca
    11101 gaggccgaag gcgaagagca gcagcgcgac aatggcgagg gtcgcccaga gccagagcgg
    11161 gatcaaaggc tggaggacga ggctggcggc ggtcactggc cgagcctttc gagcagcgcc
    11221 ggaatgtgca cctggtccgt cttgtagttg cccgtcagcg cgtacatcac gatgttgacg
    11281 ccgaagcgcc tggccatctc gcgctggcgc tcgccgcccg gcacgacggg atagagggtg
    11341 cgcccgtact cgtcctcggc ccaggcgccg gcccagtcat gggcgccgat caccacgctc
    11401 gacacaccgt cgttgacgct ttgcggcatg cggtcgaccc agaccggccg accggcgaag
    11461 cgaccgggca ggtcctgcat gaggtagaac gagcgggtca gcacgtgatc gtcggggatc
    11521 ggctccaaag gcggcagatc gaggtcgcgg agcagctcgg ccaggcgctg ctctcccggg
    11581 ccgacgatgt tctggccggg caggaggcgg gcggcgtcgc cggtgtcgaa gaggaccatg
    11641 ccgccctggc gaagatagtc ctcgatgcgg tcgatggctg cggtgtccag ggcgggatgc
    11701 tcgggtggca ccggccagta gaggagcggg aagacggcga gctcgtcgac ggcaggatcg
    11761 accgccacgg ggtcgccggt ctcgatcgag gtccgctgtt ccagcacgcg gccgaggccg
    11821 aagagcccgg cgcggctgcg ctcgtcgagg tcggcgatgc cagtctcgac atagccgagc
    11881 cgggtgatgg cggcgagctc ggcgatgcgt gcctcgtcga tcgattcctg ggcattcgcc
    11941 agcccgggga gcaggcagat ggccaggagt gcggcggcac tcgccgccgg ccgcagccgc
    12001 ggcaggaggc cgcgcagcgc gaaggagagg gcgaggtcga cgagggcgag gatggtcgcg
    12061 agcgtcagga gcggcggcag gagatcgcgc tcggtgcctt gggcataggg cagggcaccc
    12121 tcgccgaggg tggcgacgtc gaggctctcc agccggtcga gggagtcctg caggttgcgg
    12181 gcgacgcggc cgcgctcgtc ctcggccata tcccccgggg tggtgccggc atcgacgggc
    12241 gcatagaggc cggcggggct agcggggcct accggcaggt cggccagcgc tgccgcctcg
    12301 atcggctgga ggtcggccgg cggctccacc aggctgccgt cggcggcgag cacccggtcg
    12361 gcgcggacga ggccgtcgag tcgctcgccg gcgccgacgc cgagatcgac gacgcggcgc
    12421 agcatctcca cgaagagacc ggagagcggc aggctggtcc agctcgtgtt ggcggtggtg
    12481 tggacgagca agatgtagcc gtcgcccgcc ttgcggccgg tgacgatcgg cgtgccgtcg
    12541 gcgaggctcg ccagtgtcgc ctcggcgagg tccgggcccg gctcggccag cacctggcga
    12601 tagaccagcg cctcctcgct gacgtcgagc ccggcgaaga ggctgtcggc ggggaaggga
    12661 gccagcggca ggggctccga ccaggacagc gcaccgccga gctggcggtc gccggagcgc
    12721 agccggacgg gcaggagcgg atcgtgctcg acctcgccga ccggctggct cgcctcgtcc
    12781 agcccggcga gcctggggcc ggcgaaacgc agcaccacgc cgcccgtctc gacccatgcc
    12841 tcgagcgctg ccgcctcggc cggatcgaga tggccgcgat cggtgatgac gagcagcgag
    12901 agcggctcct cgaccagctc ggtgatccgc ccggtgcgca gctcggcgaa aggggcgagc
    12961 gcgcgctcga cgaaataggg ttcggcgaga agcggctgcg cggccgccgc cccgggcggg
    13021 ccggcgatgc cgaccgtgcg ccgccgccag cgctcgtcga agagcagcac gccgccgatg
    13081 ccgtgattgc cttcgagggt gaggcgggcg atgcggttgc gcacatccag cggcaggtcg
    13141 agctcgacgg tggcacgcag gggatcggcg tcgtcgccgc cgggtgccgt gatggggaag
    13201 cggccgagca cctcgccggc ggggccgatg gcgaaaagct ccggcggcag gggagcgtcg
    13261 gcggccgggc gcagcacact caccgactgg aggtcgcggg ccaggtcgtc cgggctgagc
    13321 aggagggcac gctcggccgg ggggtcggcg tggatcgaga gctcgccgat ggcaccgagc
    13381 gccgtcgcca gcgcccgggc cgcctcggcc tcgccatcgc ggtcgatgag tccgtcgctc
    13441 agccagacgg gcctggcttc gctgagggcc gatgcttcga ggcgcgccag cgccgccgca
    13501 tggtcgacgg gccagggatg gggctgccag ccggcgagcc cctccagcac ctggcgcgcc
    13561 ggctgcggct cgagcggggt ggacctgccg tccggggcgg tcttcagcac aacgaccggg
    13621 cggctgtcgc gctccgcctg ctccagcagg ccgcgggcga gaccgacccg ccgctgccag
    13681 ttcggggccg cggcccagcc atcgtcgacc accagcagca cgggaccccg gccgccgatc
    13741 accggtgccg ggtcgagcac cggcccggcg agggcgagga ggacgagggc ggcgatggcg
    13801 aggcgcagca gcaggagcca ccacggtgtc ctcgccgggg tctcctccgg tgccacgagc
    13861 ctgagcagca gggcgagtgg cgggaaggcg acccggcggg gtgcgggtgg gcggaggcgc
    13921 agcagcagcc agatcagcgg caaggcgagg agcccggcca ggatccatgg ctggaggaag
    13981 gcgatggggc ccaacgctag catcgtcaat gacggccggc ggcgcgcgga gcgagcatct
    14041 ggtagagcgt tagcaggccg gtctcggttg gtcggtcggt gcggtggcgg gcgaactgcc
    14101 aggcatggcg gcgggcgagg tcggccaggt gctcgtgatg ggcgcggtag aggtcgcggt
    14161 agtcgctgcg gatggcttcg acattgccga tcagcgcccg cccttcgccc tccagcccct
    14221 cgaagaggac gcggccttgg aagtcgagcg tctcctcggc cgggtcggcg agcatcatga
    14281 gcgacacctc ggccccgagc gcacccagcg tgcggacccg ctcggcgagg tggtccgcgg
    14341 gctcgaggaa gtcggagacg agcacgaggc gggcatggcg tggcaagggc tggggcggca
    14401 ggcgaaagcc ggcgccggcg gcaccgcgca gcagccgctc gaccaccaga ttgaagccgt
    14461 agcgaccgcc ctgcggccgc tcgccggtgc cgagcagcgc gattcgctcg ccgccgcgca
    14521 ccaggagccc agccaaggcg agcagcagca cgagggcccg ctcgcgcttg gtcgccaagg
    14581 ccggcttcga gcggtagcgc atcgacggcg actggtcggc ccagagccag acgctttcgg
    14641 ccgcctccca ttcctgctgg cggacgaaga ggtggcgcga gcgggccgac tggcgccagt
    14701 cgatggagcc cgcggcatcg cccggctggt actggcggaa ctgccagaag gtctcgccca
    14761 accccggccg gcggcggccg tgcacgccct ggctgacggt ggtggcgacc cgctcggcct
    14821 cgaccaggag gccgggcagg cggccgccga ggcgttcggc ctcgccgcgc agggccgcga
    14881 catgaccggc ctcgagtgct gccgtcggtt cagacaccat gcgccggttc aaccgacatg
    14941 ccggaccagc cgctcgatga cgtcttcgac accgatcccg tcggcgcggg cggcgaaggt
    15001 gacggccatg cggtggcgca gcaccggccc ggcgagcgcc tgcaggtcct cgagcgaggg
    15061 ggcgaggcgg ccgccgagaa gggcgcgcgc ccgggtgacc agcatcaagg cctggctggc
    15121 gcgtggcccc gggccccagg cgacctgctc ggcgatgccg tcgaccgggc tcgcctcggg
    15181 tcggccggcg cggaccagct ggagaatcgc ctcgaccagg ctctcgccga ccggcaggcg
    15241 acggacgagg gcctgcatgg ccatgagctc gggaccgtcg atcaccgcct gcggccgggc
    15301 ctcgttgccg ctggtcgtgg cgatcatcat ctgccgctcg gccgcgaccg aggggtagtc
    15361 cacatcgatc tgcatgatga agcggtcgag ctgcgcctcg ggcaggggat aggtcccttc
    15421 ctgctcgatg gggttctgcg tggcgagcac atggaagggg gccggcaggc ggtggtcgtg
    15481 gccggcgacg gtgaccttgc gctcctgcat cgcctggagc agcgccgcct gggtacgcgg
    15541 gctcgcccgg ttgatctcgt cggccatcag gagctgggtg aagatcggcc cctcgatgaa
    15601 ccggaaatgc tggcgaccgc cggaatcctc ctcgagcacc tcggagccga tgatgtcggc
    15661 gggcatgagg tcgggggtga actggatgcg cttctccgtg agcccgagca ccgtgccgag
    15721 cgtctcggcg agcctcgtct tggcgaggcc cggcacgccg acaagcaggg catggccgcc
    15781 cgcgagcagg gcgatgagca cctcctcgac cacgttctcc tggccgaaga tgacgcggcc
    15841 gatctccgct ttcatgccgg ccaaacgctc gatcgcgcga tcggcgtcct gttcgatctc
    15901 gctggcggcg gcagcggcgg tcgtgtctct ttgcaagcgc ctgttccctc gcccggcagg
    15961 ggcggtgctc gagtggcggc tcgacctttc gatctcaact ggaagagcgc acaagtcgcg
    16021 tataggtaac gaatatctgc atgacggtcg agcgatggca agggctgatg ctgagaactc
    16081 acacggctgt ggtgcggccc aaccggaggc ggctcgggaa gccttcgaga tgaggcggcc
    16141 cttcgatgtc gtggcacggc tcgagtcact gctggccaaa ggcggtgagg gagaattccg
    16201 gatggccatc gacgccgaag ggcactggca ccacgaggga cggcgaatcg ctcgacccgg
    16261 catggcgcga ctcttctcca ccgccctgcg gcgggccgcc gatggcagct actggctggt
    16321 gaccccgttc gaggccggcc gtgtcgaggt cgaggatgtg cccttcgtcg tggtcgagct
    16381 caaggtcgaa ggcgagggga aggggcggac cattcacctg cgcaccgggc tcgacgagtg
    16441 gctgcggctc gacgccgacc accctttggt catgcggcca ccgcccgggg gcggggttcc
    16501 ggcaccctac gtcaccgtgc ggccgggtct cgaggcccgc ctggcccggg cggtctacta
    16561 tcatctggtg gagctggccg agccggcggc ggatgacgaa gatctgctcg gcgtctggag
    16621 cgccgggcgg ttcgtgccgc tcgggtccgt ggaaagcggc gcctgagagt gtcggcctcg
    16681 tcctggacgc cctaccggct ggccgagcgg ctgcgcgacg cgctggccga ggaagggccc
    16741 cggcatatcg gcggggacga ggtggcgagg ccattggagc cggcgcggct gcgaccggcg
    16801 gcggtgctga tcgccctcgt cgagcggccg ggcgggccgt ccctcgtgct gacccgccgg
    16861 gccgcccatc tcaaggcgca tgccggccag accagccttc cgggcggccg gatggagccc
    16921 gaggacgtca gcgccgtggc gaccgcgctg cgcgagagcg aggaggagat cggcctgccg
    16981 ccggaccggg tcgccgtgct cggtgggctg cgctcctatg acacgatcac cggctttcgc
    17041 atccatccgg tggtcggctg ggtcgccgat ccgccggcct ggcgggccga cccggccgag
    17101 gtcgcggaaa tcttcgaggt gccgctcgcc ttcgtcctcg accctgccaa tcacacccgc
    17161 gagtcctatc tgagagacgg ccagcgtcgg tatttctatg tcctgcccca tgccgatcac
    17221 tatatctggg gtgccacggc cggtattctc gtcaacttcg cgcggctgct ccgagcctga
    17281 gagcccgcag caggggcccg acatgctgcg aattgtcatc tcatatctgc tgccgttcat
    17341 tctgccgttc gccgccttct tcctctaccg gctgctgatg acgagcggcc agccgctcct
    17401 gcggcgaaca ccctggttcg tgctctcggt gatcggcatg gcgctggtcg ccggcacgct
    17461 ggtcagcttc gcgctcttcg ggggcgaggc gcaggagggc gtctatgtgc cgccgagctt
    17521 cgaggatggc cgggtcgtgc cgggccacgt ccggccgccc ctggccgagg tgccggacga
    17581 gggcgcgccg gcggatggcg atgccagcga gccgcaatga tcgaggagcg gccgctcgcc
    17641 catgccgcat ggctccgggc cagggccagc cggcgcgtgc tcgatgcgct ggagggggac
    17701 ggaaccgagg tgcgcttcgt cggcggctgc gtgcgcgaca cgttgctcga tcccacactc
    17761 gacatcgtcg acctcgacct agccaccgtg gcgacgccgg agcgcaatct cgagcttctg
    17821 gccgacgccg ggatcaagac cttcccgacc ggccttgcac acggcacgat cacggcccac
    17881 acgggttcgc ggagcttcga gatcaccacg ctccgccggg acgtggcgac cgacgggcgg
    17941 cgcgcggtcg tcacctatac agattgcttc acggccgacg cggcaaggcg cgacctgacc
    18001 atcaacacgc tctcctgcga ccgcttcggg cgcgtcttcg atacttttgg cggccgcgcc
    18061 gacctcgctg ccgggatcgt ccgtttcgtc ggcgagccga cggcccggat ccgcgaggac
    18121 tatttgcgga tcctgcgctt ctttcgcttc caggcccgtt tcgggcgggt gccgccggat
    18181 acggcgacgc tggccgccct ggcggctgaa cgggccgggc tcgacctcct ttcgggcgag
    18241 cgggtgcgga gcgaggtgct gcggctgctg atggcgccgg gcgtgctcgc ggcgctggag
    18301 ctgatgcggt cctgcggcgt ctggcgccag gttttcgccg gcgagccggc cttggaagat
    18361 ctccggcgac tgctcgacct cgcaccggac gcgacggcga tcctgcggct cgcctgcctg
    18421 ctgcgggacc ggagcgaccc cgctgccgtg gcggcccggc tcaggctctc gaacgccgag
    18481 cgggagcggc tggctcaggc gatgtcgggc gagcttcctg ggatcgatgc aggaccggtg
    18541 acgctgcgcc ggctggccta ccggctcggc accgaaggcg ccgacgaccg gctggtggtt
    18601 gcggccgccc gcactgctgt ggcacccgac acggttgcgg aggcccggcg cattgtccgg
    18661 gatttcggtc ggcgatcgct gccggtcggt ggcaaggacc tgctcgcccg cggcatggcg
    18721 ccggggccgg ggctgggggc caggctgcgg gagctggagg acgcctggat cgccagcgat
    18781 ttcaagttgg accgggagat gctgttgaag caattgggtg gaggggacca ttgagcgggc
    18841 gcttcgcggt ggtcggcgcc ggagtggccg gggtcgcctg cgcccgggca ctggccgatg
    18901 cgggctggcg agtagcgatc cacgagcgcg agccccggat cggtggccgc ttggcacagt
    18961 gccaccaaga gggcgccgtc ttcgattacg gcggccagtt cgtccggccg cgcgacccgg
    19021 ttctgaaggc gctgctcgag gccggcgtcg ccgccggcgt cgccgctcgc tggccggcgg
    19081 cggaccgcga cggggtggcg gcctatgtcg gccgcccgac aatggcagcg cctctggagg
    19141 cgctgctgca tgggatcgag accaggaccg gctgtcagat tgtcaggctc tcgcgagatg
    19201 gcgggtcctg gtggctcgag gacgagggcg gtgcccggca cgggccgttc gaccgcgtag
    19261 ggctggccct gccgccggtc atcgcgggcc agctcctggg caccgtcgag gcgccgcctg
    19321 aggcgctcgt tgccgcaaca gcagcggcgg ctttggcgcc ctgctgggcg ctgctgctgg
    19381 ccttcgaccc gccactcgac ctccacggat tcgatgcgcg ggtgatcgat gaggggccgc
    19441 tcgcctggat cgcgcggaac accaccaagc gaggccggca agggcgcgac tcctggacgg
    19501 cgcatgcgac gcaggattgg tccgccgcgc atctcgagct gtcgccggag gcggtcctcg
    19561 agcagctcgt gccggcattt cgggcggtga cggggacggc ggatgttcca ttccatgcgg
    19621 gagcgcattg ctggcggcag gcccaggtga cccgggcact cggcgtggcc gcggtgcatg
    19681 atccgggggc cgggctcggc ctttgcggcg actggtgcct cagccgcaac gtggaggcag
    19741 ccttcctttc cggccgagcg ctggccgggc tcctcggcga aggcggctga tgagtgcgat
    19801 ttgacgtccc cggccggctt gcttttgtcg gtcggcgcgc gtagagagcg caccatctct
    19861 cacgcggatc gctagcgggc cacgtgcctg caccggtgtc cggcgccagc cggatcgaga
    19921 tccgcggcgg ctcaaccgga cacagaaaga cgaccaatgg ctttcccgac cttcacgatg
    19981 cgccagctgc tcgaggctgg cgtgcatttc gggcatcaaa cgcggcgctg gaacccgcgg
    20041 atggagccct acatcttcgg cgtgcgcaac ggggtgcata tcctcgattt gacgcagacc
    20101 gtgcccctga tgcatcgggc gctgcaggcg gtgcgcgacg tcgtc
//
LOCUS       DAOTFI010000068        20066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3243_length_20066_cov_6.264005, whole genome shotgun sequence.
ACCESSION   DAOTFI010000068 DAOTFI010000000
VERSION     DAOTFI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 20066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20066
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3243_length_20066_cov_6.264005"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="P5558_08785"
     CDS             complement(<1..244)
                     /locus_tag="P5558_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY24443.1"
                     /translation="MKDHYLVTIRLIERLHRRFLDVIKAELDRLGIEDINNVQTLILS
                     NINADQLTVGELTVRGYYLGSNVSYNVKKLVENGYLI"
     gene            complement(311..1204)
                     /gene="mmsB"
                     /locus_tag="P5558_08790"
     CDS             complement(311..1204)
                     /gene="mmsB"
                     /locus_tag="P5558_08790"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HRY24444.1"
                     /translation="MARIGFLGLGNMGLPMASNLRRAGHAVTGFDPGDPARRAAGEAG
                     LRLADDLAGAVVGAEVVISMLPAGPHVRDAYEGPAGVLTHAMPQALLIDCSTIDVETA
                     RAVARAAAARGHDMVDAPVSGGVTGAAAASLTFMVGGTVEAFGRAAPFLEAMGQTIVH
                     AGDAGTGQAAKICNNLMLGIQMISVCEAMVLAEKLGLAHQKLFDISSRSSGQCWSLTS
                     YCPVPGPVPTTPANRDYRPGFTAAMMLKDLKLAMGAAEMAGAATPMGAAATQLYAKFA
                     REENEGLDFSAIIKMIRGTPG"
     gene            complement(1204..2259)
                     /locus_tag="P5558_08795"
     CDS             complement(1204..2259)
                     /locus_tag="P5558_08795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRY24445.1"
                     /translation="MSGTVHVEVQGGLGILTLDRPRALNALDRGMVRSVSKALRRWRD
                     DDGIRAVLIRAVPGRAFCAGGDIVGVVETAKTEGAAAAAPFFFDEYRMNWRIGNLGKP
                     FIALLDGITMGGGVGISVHGSHRIATENTLLAMPETGIGFFPDVGGSHFLPRLPAHAG
                     LYLGLTGERVDGATATRLGIATHFVPAASLPALEAALVDGEGIDAALARFADEPVPGG
                     LDAVAIDRLFAAASLDELLAGLARDGGAFATGTLETLRQKSPLSVAVTFEELRRGAGL
                     DLAGCLAMEYRLVHRFLEGHDFPEGVRALLVDRDKQPRWQHATIEAVNEAEIAACFAP
                     LVGGDLEFDWQPRGEDD"
     gene            complement(2256..2462)
                     /locus_tag="P5558_08800"
                     /pseudo
     CDS             complement(2256..2462)
                     /locus_tag="P5558_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643854.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            2563..3579
                     /gene="hemB"
                     /locus_tag="P5558_08805"
     CDS             2563..3579
                     /gene="hemB"
                     /locus_tag="P5558_08805"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRY24446.1"
                     /translation="MSQPCHLPPLPPFPHSRPRRLRRAAWSRRLVAEHCLRPADLILP
                     VFVMDGSDCEVSIGAMPGVVRHSPDRVLRVAERALEAGIPALALFPATPLEAKTEDGR
                     EAWNPENLVCRTLRLLKRQLPELGLIADVALDPYTTHGQDGLVDGGVVLNDETLEALC
                     RQALCQAEAGCDVIAPSDMMDGRIRAVREALDAAGHQETLILSYAAKFASAFYGPFRA
                     AVGSATALGAADKKTYQMDPANSSEAIREASLDIAESADMLMVKPGMPYLDVIRTLKD
                     AFHLPTLAYQVSGEYAMLKAAGQNGWLDGDAALLESLLCFKRAGADAILTYAALEVAG
                     RLGR"
     gene            3703..4836
                     /locus_tag="P5558_08810"
     CDS             3703..4836
                     /locus_tag="P5558_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24447.1"
                     /translation="MPEPTPDEPMRRAIVLVPGIQRVERFVRRDRLVDNLATVERHPL
                     AAGGEVELAGEIGRRLLPGSLRGVAARPVETDVFEAYWGDMVDDPVETGPWGKLWSGF
                     ELVAYWVFSRGTWRAFALSRFITIGLLFSGLLLILWYVSIALLVASTLRGMQPPEVLA
                     LPVLAQLYEGFLWLAAGIESFAYWALLAFVLSAVNADGLAQIGRFTKDYLENRPNEDE
                     VGLRDRLRCRVRSTLDAVLAEPYDEVVVAAHSLGSVIALDALADWPHEADWPRLRLVT
                     LGSPVAVLACRSSWLARERQKLLDRPPVTWLDFHSNADWLCTSVIAGLGGTVPAGVQS
                     FELPFDAGLLGRLSGSSHQDYYRNQRVLETLATPGLLPAAPQI"
     gene            4865..6631
                     /locus_tag="P5558_08815"
     CDS             4865..6631
                     /locus_tag="P5558_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HRY24448.1"
                     /translation="METHAHIPYLRELVVFLIAAGVIVPVLHRLRISPVLGYLVIGAL
                     IGPFGLGRLAGAWPWLGHVVITDLDGVKALAELGVVFLLFMIGLELSIERLWSMRRLV
                     FGLGSLQILASGLVIGLIAWGFGNTPRASIVLGACLALSSTAIVMQLLIEGRRIGTMV
                     GRSSFSILLMQDLAVVPILFLVGVLGARTGGNVGLDLVLAIGKAVLVILAIYLVGRMV
                     LRPLMRLVAQAHSPEMFMAAILLTVIGTAAITGAAGLSMALGAFLAGLLLAETEFRHE
                     VEIDIAPFKGLMLGLFFMSVGMGIDYRMIAAEPLWIIGSVFGLYLVKSLITAGLCLAF
                     GLPRHTALETGLLLGEGGEFAFVVVGLAMSLGLIGGDVGQFMLIVAGLSMVATPLVAR
                     FATRLANAVEKRAAAEAVPATQRQIEGIEGHVVLAGFGRVGRTLGDMLDEQSIPYLAI
                     DGDPGTVHRAREAKKRVFYGDASRVEMLNRVRADTAAAIVVTTDAPDAIERIVHAAHS
                     LFPDVPIYARARDTLQARRLMAAGATLAVPETVEASLQLAGRVLQGFGLPDESVNRLI
                     EQQRRLLEPTNPNHPPGFAVLR"
     gene            complement(6633..7478)
                     /locus_tag="P5558_08820"
     CDS             complement(6633..7478)
                     /locus_tag="P5558_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY24449.1"
                     /translation="MMREAALTIDDAGAPLALGYVEWGDPAAPRTVLCLHGLTRNARD
                     FDFLAQALAGTARVLALDVAGRGRSGWLADPARYAVPTYVGHVAAVLDGLGVAAVELV
                     GTSMGGIIGMGLAAHQPARVAKLVLNDIGGRVPVAALVGIADYLRDQPPLFADMAELE
                     AHLRRVHAPFGPLADAQWAHLARHSARPVPGGLALHYDPAIAAPFRETATADLDLWPL
                     YDAVTCPTLLLHGAESLLLPAEVAAEMTGRGPKARLVTFAGIGHAPALMAADQIAVIR
                     NFLED"
     gene            complement(7492..8433)
                     /locus_tag="P5558_08825"
     CDS             complement(7492..8433)
                     /locus_tag="P5558_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY24450.1"
                     /translation="MSTLAIVAVEPSRVARGIGMSLLAYASFSTADALLKAFSGAFSV
                     FQIAFTMAFFAMLPVLATTIGRGGLRSLVPRNKPLVAARGILAAICGCLAWSAFALLP
                     MAEVYALLFAAPMLVTGFSAILLRESVGWRRWSATLLGFAGVLVMIDPEFSSLGLGHA
                     LAGLAALAGALSFIILKKIGAAEKSGAMLMSVMLWLMAVALPPALLAWVWPSPAELGS
                     MAMAGLLMGAGQAGLVFATREAPAVVVAPFQYSQMIWAVVFGMLLFGDVPGTNLFVGL
                     GIVVACGLFIVYRETVRSRPVTVGGGRGEVPARIARV"
     gene            complement(8585..9031)
                     /locus_tag="P5558_08830"
     CDS             complement(8585..9031)
                     /locus_tag="P5558_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY24451.1"
                     /translation="MIKPSKKVALAVEAVVDIAYHGTSEPVQSQDIGRRLNLPRRYLE
                     QVMQQLVKAGILRGVRGPRGGYRLARERRRITVADIVNVVESGEAEPDTLSASDLGQR
                     VMAPFWLEHEQAMLARLDAITIEDLCHAAEQSAVRREIKDGIDFAI"
     gene            complement(9028..9549)
                     /gene="dut"
                     /locus_tag="P5558_08835"
     CDS             complement(9028..9549)
                     /gene="dut"
                     /locus_tag="P5558_08835"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRY24452.1"
                     /translation="MRIDVTIERLAHGEGLPLPAYATALAAGADLHAAVPADGPVALD
                     PGQRALVPTGLRIALPEGCEAQVRPRSGLALKHGVTVLNAPGTIDADYRGEIGVVLVN
                     LGQETFRVRRGERIAQLVLAPVARARWLEATGTLSVSALSVSDRAAGGFGSTGSRPLH
                     LGTEPATKGSQTR"
     gene            complement(9546..10751)
                     /gene="coaBC"
                     /locus_tag="P5558_08840"
     CDS             complement(9546..10751)
                     /gene="coaBC"
                     /locus_tag="P5558_08840"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRY24453.1"
                     /translation="MNAKRILLVIGGGIAAYKTLELIRRGRERGLAFRTILTRAGAEF
                     VTPLAVSSLSESKVYSDLFSLTDEAEIGHIRLSREADLVVVAPATADLMAKMAQGHAD
                     DLASTTLLATDKPVLVAPAMNPMMWQHPATQRNVARLRADGIHFVGPAAGDMACGEVG
                     SGRMTEPAEILAAIHAILVPPGERPLAGRRAIVTSGPTHEAIDPVRYIANRSSGRQGH
                     AIAAALAAAGAEVTLVSGPVSLPDPPGVAVRRVESARAMRDAVEAALPADIAVCAAAV
                     ADWRPAQAAEAKLKKGRNQPAALELVENPDILRGLGHHPQMRPELVVGFAAETDDLLA
                     NAAAKLERKGCDWILANDVSTGSGVFGGSRNRVHLLGSEAPVESWPEMDKDELATRLV
                     ARIAERLGR"
     gene            complement(10833..12332)
                     /gene="ubiB"
                     /locus_tag="P5558_08845"
     CDS             complement(10833..12332)
                     /gene="ubiB"
                     /locus_tag="P5558_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HRY24454.1"
                     /translation="MLESPRYLWRLVAIGRTLARHNALFPFELLPQARPLLRLARPFV
                     NENAAGRPGQRLAAALEELGPSFIKFGQSLATRTDLLGEAIARDLATLQDALPSFPAA
                     AARATIATDLEQPVEALFSDFQDRPVAAASIAQVHLATTTDGDEVAVKVLRPGIEKAI
                     ERDLGFFAWLARLVERTQPHLRHYKPVEAVAIFAQVTRRELDLRLEAAAAVELAENAE
                     GYEGFRVPAVDWRRTGRRVVTFERIVGQGITDRAALIAAGHDPDRIMENAAKAFFSQV
                     FKDGFFHGDMHPGNILIDREGTIVALDFGIMGRVDMADRRHMAGILVGFLERDYVKVA
                     DVFFAAGFLPDDADRMAFVQAVRSIGEPILGLPLEEISFGRLMGQLLAMAEQFRMQTQ
                     PQLLLLQKTMVVAEGVGRLLNPKVNMWQLAQPLVEDWIIAHLGPKARLQQALDDGLQM
                     AGRLPHIVRRLDEALERERTRHVAVDRVKLFQGWFWPLVVGVLIGLAVG"
     gene            complement(12355..13131)
                     /gene="ubiE"
                     /locus_tag="P5558_08850"
     CDS             complement(12355..13131)
                     /gene="ubiE"
                     /locus_tag="P5558_08850"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRY24455.1"
                     /translation="MEKTDRPAAAAAELASSFGFEAVAPAVKAGRVRQVFDSVAGRYD
                     LMNDLMSFGVHRLWKEAVLDWLAPRPGIHLLDLAGGTGDVAERVLRRVGGDGRAVLAD
                     INAAMLGQGRDRALGRGWFDRLAFVCADAMALPFEAGRFDAVTMAFGIRNVTHVDRAL
                     AEGFRVLRPGGRFMCLEFSKVVLPVLDRVYDTYSFQVLPALGRIVAGDAASYRYLAES
                     IRRFPDQPTFADLMRDAGFEQVRWRNLSGGIAAIHSGWRL"
     gene            13234..14049
                     /gene="mutM"
                     /locus_tag="P5558_08855"
     CDS             13234..14049
                     /gene="mutM"
                     /locus_tag="P5558_08855"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRY24456.1"
                     /translation="MPELPEVETTLRALAHRLDARRITGVEQRRADLRFGLPAGLPAR
                     LAGRRLTGFARRAKYLQLMLDDGMAMLLHLGMSGRLLFDGPPAGPHEHLTFHFDDGTI
                     LRFVDPRRFGMLDLSPQATLGEHKWLAHLGLEPLGNAFDGAALRDRLAGRQVALKIAI
                     MDQRHVVGVGNIYASESLHRAGLSPRRLACTLGPARAQRLAASIREVLLEAIDAGGSS
                     LRDYVQSNGELGNFQRQFRVYDRAGLPCTGCGRPVARLVQGGRATFFCGRCQR"
     gene            14217..14990
                     /locus_tag="P5558_08860"
     CDS             14217..14990
                     /locus_tag="P5558_08860"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRY24457.1"
                     /translation="MAFETILVDKRDTIAVITLNRPKQLNALNGQLIDELRRALASLD
                     ADPAIAAIVLTGSDKAFAAGADIKEMAELDFAQAYGSDFIGAWHRIAEHRKPVIAAVA
                     GYALGGGCELALMCDIILAAENAKFGQPEINLGTIPGAGGTQRLTRAIGKSKAMEMIL
                     TGRNMDAVEAERANLVARVVPLGDLLDEAVALAQAIATKSQPIVAMAKEAVDVAYETT
                     LAEGIRFEKRSFYATFATADRREGMLAFAEKRSPNFAQS"
     gene            15080..15358
                     /gene="rpsT"
                     /locus_tag="P5558_08865"
     CDS             15080..15358
                     /gene="rpsT"
                     /locus_tag="P5558_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRY24458.1"
                     /translation="MAHSRTARKRIRQTAKRTELNKSRISRIRTFVRKLDDALARGDA
                     PAATAAFAAAAPELQRGVTKGVLHRNTVARKISRLSQRVRLLATSQPG"
     gene            15858..17339
                     /gene="dnaA"
                     /locus_tag="P5558_08870"
     CDS             15858..17339
                     /gene="dnaA"
                     /locus_tag="P5558_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRY24459.1"
                     /translation="MAGPLGAELTQQWSRVRGLLRAEFGDTAYSTWLRPLEAIALDGD
                     RVLIVVPTQFMRDWVVNNYADRIRAHWTSVNPTVRSVALDVRPARLDPTRLDPARLDD
                     SGRLAGRPATKPRIVADNVREAHAEGAPPAVESREPGKAADRQAGPAELAEFGAGLDP
                     RFTFDTFIVGKPNEFAVAAAERMALSATPPFNPLFLYGGVGLGKTHLMHAIGWRAKQN
                     DPDRRVVYLSAEKFMYQFVRAIRQRDTMGFKEMFRSVDLLMVDDVQFISGKDSTQEEF
                     FHTFNALAERGSSIVISADRSPSDLAGLEERIRSRLGWGLVADIHPTTYELRLGILQA
                     KAEQLGVEVEPEVTEFLAHRITSNVRELEGALNRIVHQATLIRRPVSIEMAQDVLRDL
                     LRSNERRVTIEEIQRAVVEHFQLKMADMSSTRRARAVARPRQVAMYLAKQLTPRSLPE
                     IGKKFGNRDHTTVMHAVKQIEKLCEEDRSLADDVAALKRRLAG"
     gene            17491..18615
                     /gene="dnaN"
                     /locus_tag="P5558_08875"
     CDS             17491..18615
                     /gene="dnaN"
                     /locus_tag="P5558_08875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRY24460.1"
                     /translation="MKLAIERAALLRSLSHVQAVVERRTTIPILSNVKLAAGGDGLHL
                     TATDMDLSMASSETARVQDDGVTTVSAHTLFDIVRKLPDGSEVLIEQEPNASEISVRA
                     GRAVFTLPCIKADEFPAMDGDAEAYDVTFDMPAADLRKLIDKTRFAISTEETRYYLNG
                     IHVHASGAGATAVLRGVATDGHRLAKIEIALPDGAEGIPSIIIPRKTVTELRKLIDAI
                     DGEIRVKVSTNRIQFVLDQAVLVSRLIDGTFPDYERVIPVGNEKVAAIDTKLFAETVD
                     RVSTISTERARAIKLRFDDGRITASAVSTEAGRAVEELDADYAGEPLEIGFNSRYILD
                     MLAEVAGDQVRLEMANPAAPTLVRDPADGSVVYVLMPMRV"
     gene            18673..19842
                     /gene="recF"
                     /locus_tag="P5558_08880"
     CDS             18673..19842
                     /gene="recF"
                     /locus_tag="P5558_08880"
                     /inference="COORDINATES: protein motif:HMM:NF000820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRY24461.1"
                     /translation="MSPLREAARARPTRETDPARPALERLRLRDFRNYAGLELELAPG
                     PIVLFGPNGAGKTNLLEAISLLAPGRGLRRARLEALDRDGAAAPFRLEAGLRTPDGPV
                     DVASGREPESDRRWLEIAGQPVRGLAALADLVAITWLTPAMDRLFVDAAAERRRFLDR
                     LVLGVYPDHARRLAIFERAMRERSLVLRQGPRDATWLDILEQRMAESGVAIAAGRREL
                     AAGLGTLLAEPDPALPRLRLELDGSVETWLDDMAALDAEQRLADALAAARAQDTEAGG
                     AAIGPHRSDLVAIDLATNEAAPRVSTGRQKAMLLAIVLAEVRLRELVAGDLPILLLDE
                     TAAHLDPTRRAGLAERLLALGAQVLLTGTERHLFSGLDGHATFIHVDRGLIRADE"
     gene            19835..>20066
                     /locus_tag="P5558_08885"
     CDS             19835..>20066
                     /locus_tag="P5558_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24462.1"
                     /translation="MSDTPELPTEPQTGRTGAATASATGGEPIYDSTSIRVLEGLEAV
                     RLRPGMYIGDTDDGSGLHHMVYEVVDNAIDEAL"
BASE COUNT         3039 a         7299 c         6775 g         2953 t
ORIGIN      
        1 ggatcaggta gccgttctcg accagcttct tcacgttgta ggagacgttg gagccgagat
       61 agtacccgcg gacggtgagc tcgcccacgg tgagctggtc ggcgttgatg ttcgagagga
      121 tcaacgtctg cacgttgttg atatcctcga tgccgagccg atcgagctcg gccttgatga
      181 cgtcgaggaa gcgccggtgc agacgctcga tcaggcggat ggtgacgaga tagtggtctt
      241 tcactcagcc ctcggcgcgc gattgcatca tgcgtcccct tttccccgca gccggaccac
      301 cccgcctagg tcaaccggga gttcctctaa tcatcttgat aattgctgaa aaatctagtc
      361 cctcgttctc ctcgcgggcg aacttggcgt agagttgcgt cgcggcggca cccatcggcg
      421 tcgccgcccc ggccatctcc gccgcgccca tcgcgagctt caggtccttg agcatcatcg
      481 ccgcggtgaa gccgggccgg tagtcgcgat tggcgggtgt ggtcggcacc ggacccggca
      541 ccgggcaata ggaggtgagc gaccagcact ggccggacga ccggctgctg atgtcgaaga
      601 gcttctggtg cgcgagcccg agcttctcgg ccagcaccat ggcctcgcag accgagatca
      661 tctggatgcc gagcatcagg ttgttgcaga tcttggccgc ctggccggtg ccggcgtcac
      721 cggcatggac gatcgtctgg cccattgctt cgaggaaggg ggcggcgcgg ccgaacgcct
      781 ccaccgtgcc gccgaccatg aaggtgagcg acgcggcagc ggccccggtc accccgccgg
      841 agacgggtgc gtcgaccatg tcgtggccgc gggcagccgc ggccctggcc accgcccgcg
      901 cggtctcgac gtcgatggtc gagcagtcga tgaggagcgc ctgcggcatc gcatgggtga
      961 ggacgccggc gggcccctcg taggcgtcac ggacatgcgg cccggccggc agcatgctga
     1021 tcacgacctc ggccccgacc accgcaccgg cgaggtcgtc cgccaggcgc agccccgctt
     1081 ccccggccgc ccggcgggct gggtctccgg gatcgaaacc cgtcaccgcg tgcccggccc
     1141 gacgcaggtt cgaggccatt ggcaggccca tgttgccgag cccgagaaag ccgatccgtg
     1201 ccatcagtcg tcctcccctc gcggctgcca gtcgaactcg agatcacccc cgacgagcgg
     1261 tgcgaagcag gccgcgatct ccgcctcgtt caccgcctcg atcgtcgcgt gctgccagcg
     1321 cggctgcttg tcccgatcga ccaggagggc gcggacacct tcgggaaagt cgtggccctc
     1381 gaggaagcgg tgcaccaggc gatactccat ggcgaggcag ccggcgagat cgagcccggc
     1441 gccgcgccgc agctcctcga aggtcacggc cacgctgagc ggcgattttt gccggagcgt
     1501 ctcgagcgtc ccggtggcga aggcaccgcc gtcgcgggcg aggccggcga gcaactcgtc
     1561 gaggctggcc gcggcgaaga gccggtcgat ggccacggca tccagcccgc cgggcaccgg
     1621 ctcgtcggcg aagcgggcca gggccgcatc gatgccctcg ccgtcgacca gcgccgcctc
     1681 gagggccggc agggacgcgg ccggcacgaa atgcgtggcg atgccgagcc gcgtcgcggt
     1741 cgccccgtcc acccgctcgc cggtgagccc gagatagagg ccggcatggg ccggcaggcg
     1801 cgggagaaag tggctgccgc cgacgtccgg gaagaagccg atgccggtct cgggcatggc
     1861 gaggagcgtg ttctccgtcg cgatccggtg cgagccgtgc accgagatgc cgaccccgcc
     1921 gcccatggtg atgccgtcca gaagggcgat gaagggcttg cccagattgc cgatccgcca
     1981 gttcatccgg tattcgtcga agaagaaggg tgcggcggcc gcagctccct ccgtcttcgc
     2041 cgtctcgacc acgccgacga tgtccccgcc ggcgcagaag gcccgccccg gcacggccct
     2101 gatcagcacc gcccggatcc cgtcgtcgtc gcgccagcgc cgaagtgctt tggagacgct
     2161 ccgcaccatg ccgcggtcca gcgcgttgag cgcccgcggc cggtccagcg tcaggatgcc
     2221 gagcccgccc tgcacctcga catgcacggt gccgctcatg gccggagcag ccggcgggcg
     2281 atgatcagcc gcatgatctg gttcgttccc tcgaggatct ggtggaccca cgcgtcccga
     2341 aagaagcgct cgatgccgtg gtcgcgcaga tagccgtagc cgccgaaaag ctgcaaggcc
     2401 cggtcgatca ccgcgaagcc cgtttcggtg tcgagccgct tggccaaggc gatgagggac
     2461 cggagtccat cgctgccccg catccccgtc caggccgttc cgattgcccg gcggccccga
     2521 gagatataaa gattccagct tcacgaccag tggacgaaca ttttgagcca gccctgccat
     2581 ctgccgccgc tcccgccctt cccgcattcg cgcccgcgcc gcctgcgccg cgccgcctgg
     2641 agccggcggc tggtcgccga gcactgcctc cggcccgcgg acctgatcct gccggtcttc
     2701 gtcatggacg gcagcgattg cgaggtgagc atcggcgcca tgccgggggt ggtgcgccac
     2761 agccccgacc gggtgctgcg cgtcgccgag cgggcgctcg aggccggcat cccggccctg
     2821 gccctcttcc cggcgacccc gctcgaggcc aagaccgagg atgggaggga ggcctggaac
     2881 ccggagaacc tcgtctgccg cacgctccgg ctactcaagc gccagctccc ggagctcggc
     2941 ctgatcgcgg acgtggcgct cgatccctac accacgcacg gccaggacgg cctggtcgac
     3001 ggtggtgtcg tgctcaacga cgagacgctc gaggccctct gtcgccaggc gctctgtcag
     3061 gccgaggcgg gctgcgacgt catcgccccc tccgacatga tggatggccg catccgcgcc
     3121 gtgcgcgagg cgctcgatgc ggccgggcac caggagacgc tgatcctctc ctacgccgcc
     3181 aagttcgcct cggccttcta cggcccgttc cgcgccgcgg tcggctcggc cacggcgctg
     3241 ggtgctgccg acaagaagac ctaccagatg gacccggcca acagcagcga ggcgatccgc
     3301 gaggcctcgc tcgacatcgc cgagagcgcc gacatgctga tggtcaagcc cggcatgccc
     3361 tatctggacg tcatccgcac gctgaaggac gccttccatt tgccgacgct cgcctatcag
     3421 gtctccggcg agtacgcgat gctcaaggcc gccggccaga acggctggct cgacggcgac
     3481 gcggcgctcc tcgaaagcct cctctgcttc aagcgcgccg gcgccgacgc catcctcacc
     3541 tacgccgcgc tggaggtggc gggccggctg gggcggtagg cgcggcctgt ctcgaacgag
     3601 cgcagggatg agcctgatcg acaccaccca ccgccgttga catcgtccct gcaatccccg
     3661 gttatggcat cagcgatccc cgcttcccgg aggaagtgat cgatgcccga gccgacgccc
     3721 gacgagccca tgcgccgcgc catcgtgctc gtgcccggca tccagcgggt cgagcgcttc
     3781 gtgcggcgcg atcggctggt cgacaacctc gccaccgtcg agcgccaccc cctggcggct
     3841 ggcggcgagg tggagctcgc gggcgagatc ggccggcggc tcttgccggg cagcttgcgc
     3901 ggcgttgcgg cacgaccggt ggaaaccgac gtcttcgagg cctactgggg cgacatggtc
     3961 gacgacccgg tggagaccgg tccctggggc aagctctgga gcggcttcga gctggtcgcc
     4021 tactgggtat tcagccgtgg cacctggcgg gccttcgctc tttcccgctt catcaccatc
     4081 ggcctgctgt tcagcggcct gcttttgatc ctctggtacg tgtcgatcgc gctcctggtg
     4141 gccagcacgc tgcgcggcat gcagccaccc gaggttctcg ccctgccggt cctggcgcag
     4201 ctctacgagg gtttcctctg gctcgccgcc ggcatcgaga gcttcgccta ctgggcgctc
     4261 ctcgccttcg tcctctcggc cgtcaatgcg gacgggctgg cgcagatcgg ccgattcacc
     4321 aaggactatc tcgagaaccg ccccaacgag gacgaggtgg gcctgcgcga ccggctgcgc
     4381 tgccgggtgc ggagcacgct cgatgcggtg ctggccgagc cctatgacga ggtcgtcgtc
     4441 gccgcccaca gcctgggctc ggtcatcgcg ctcgatgccc tggccgactg gccgcacgag
     4501 gccgactggc cgcggcttcg cctcgtcacc ctgggctcgc ccgtggccgt cctcgcctgc
     4561 cgctctagct ggctggcccg cgagcggcag aagctgctcg accggccacc ggtgacctgg
     4621 ctcgacttcc acagcaatgc cgattggctc tgcaccagcg tcatcgccgg cctcggcggc
     4681 accgtaccgg ccggcgtcca gagcttcgag ctgccgttcg atgcgggcct gctcggccgg
     4741 ctcagcggca gcagccacca ggactactac cgcaaccagc gagtgctcga gaccctggcc
     4801 acaccgggcc tgctgccggc agctccgcag atctgaccac cggacgaacc cttcaggaga
     4861 cgacgtggag acccacgccc atatccctta cctgcgcgag ctcgtcgtct tcctgatcgc
     4921 cgctggcgtg atcgtgccgg tcctgcaccg gctgcggatc agcccggttc tcggctatct
     4981 ggtcatcggc gccctgatcg gccccttcgg cctcggccgc ctggcgggcg cctggccctg
     5041 gctcggccat gtcgtgatca ccgacctcga cggggtgaag gcgctggccg agctcggcgt
     5101 cgtcttcctc ctcttcatga tcggcctgga gctctcgatc gagcgcctgt ggagcatgcg
     5161 ccgcctggtc ttcggcctcg gcagcctgca gatcctggcg agcggcctcg tcatcggcct
     5221 gatcgcctgg gggttcggca acacgccccg cgcctcgatc gtcctcggcg cctgcctcgc
     5281 cctctcctcg accgccatcg tcatgcagct cttgatcgaa ggccggcgca tcggcaccat
     5341 ggtcggccgc tcgagcttct cgatcctgct catgcaggac ctggccgtcg tgccgatcct
     5401 cttcctcgtc ggcgtgctcg gtgcccgtac cggcggcaat gtcggtctcg acctcgttct
     5461 cgccatcggc aaggcggtgc tggtcatcct cgccatctat ctcgtcggcc gcatggtcct
     5521 gcgcccgctg atgcggctgg ttgcccaggc ccacagcccc gagatgttca tggcggcgat
     5581 cctgctcacg gtcatcggca ccgccgccat caccggtgct gccggcctct ccatggcgct
     5641 cggcgccttt ctcgccggtc tgctgctcgc cgagaccgag ttccgccacg aggtcgagat
     5701 cgacatcgca cccttcaagg gcctgatgct cggcctcttc ttcatgtcgg tcggcatggg
     5761 catcgactac cggatgatcg cggccgagcc tctctggatc atcggctcgg tgttcggcct
     5821 ctacctcgtc aagtcgctga tcacggccgg tctctgcctg gccttcggcc tgccccggca
     5881 cacggctctg gagaccgggc tgctcctcgg agagggcgga gaattcgcct tcgtcgtcgt
     5941 cggtctcgcc atgagcctcg gcctcatcgg cggcgatgtc ggccagttca tgctgatcgt
     6001 cgcggggctc agcatggtcg cgacgccgct cgtagcgcgt ttcgcgacgc gcctcgcgaa
     6061 cgccgtcgag aagcgcgccg ccgccgaggc ggtgcccgcg acgcagcgtc agatcgaagg
     6121 catcgagggc catgtcgtgc tcgccggctt cggccgcgtc ggccgcaccc tcggcgacat
     6181 gctggacgag cagtcgattc cctatctcgc catcgacggc gaccccggca ccgtgcaccg
     6241 cgcccgcgag gccaagaagc gcgtcttcta cggtgacgca tcgcgcgtgg agatgctcaa
     6301 ccgcgtccgt gccgatacgg ccgccgccat cgtcgtcacc acggatgcgc ctgacgcgat
     6361 cgagaggatc gtgcacgccg cccacagcct ctttccggac gtgccgatct atgcccgggc
     6421 gcgcgacacc ctgcaggcgc ggcgcctcat ggcggccggc gccacgctcg ccgtgcccga
     6481 gaccgtggaa gccagcctgc agctcgccgg ccgcgtgctc cagggcttcg gcctgccgga
     6541 cgaatcggtc aaccggttga tcgagcagca gcgccggctc ctcgagccga ccaaccccaa
     6601 tcacccgccg ggcttcgcgg tgctccgctg attcagtctt cgaggaagtt ccggatgacc
     6661 gcgatctgat ccgcggccat gagcgccggt gcatggccga tgccggcgaa ggtcaccagc
     6721 cgcgccttcg gtccgcgccc ggtcatctcg gccgccacct ccgccggcag gagcaggctc
     6781 tccgccccgt gcagcagcag cgtcgggcag gtgacggcgt cgtagagcgg ccagaggtcg
     6841 aggtcggccg tggccgtctc gcgaaacggc gcggcgatcg ccgggtcgta gtggagcgcc
     6901 agcccgcccg gcaccggccg ggcgctgtgg cgggcgagat gcgcccactg ggcatcggcc
     6961 agggggccga agggcgcgtg cacccggcgg agatgggctt cgagctcggc catgtcggca
     7021 aagagcggcg gctggtcgcg cagatagtcg gcgattccga ccagcgccgc gaccggcacg
     7081 cgcccgccga tgtcgttgag caccagcttt gcgacccggg ccggctggtg ggcggcgagc
     7141 cccatcccga tgatcccgcc catcgacgtg ccgaccagct cgaccgcggc gacgccgagc
     7201 ccgtccagga ccgctgccac atgcccgaca taggtcggca ccgcatagcg tgccggatcg
     7261 gcgagccagc cgctccggcc acgcccggcc acatcgagcg ccagcacccg cgccgtgccg
     7321 gcgagcgcct gcgccagaaa gtcgaagtcg cgcgcattcc tggtcaggcc gtgcaggcag
     7381 agcacggtgc gcggggccgc cggatcgccc cactcgacat agccgagcgc caggggtgcc
     7441 ccggcatcgt cgatcgtcag cgccgcctcg cgcatcatcc ggagctcccg ttcagacgcg
     7501 ggcgatccgc gccggcacct cgccacgacc gccacccacc gtcaccggcc gcgagcgcac
     7561 cgtctcgcga tagacgatga agaggccgca ggccacgacg atgccgaggc cgacgaagag
     7621 gttggtgccc ggcacgtcgc cgaagagcag catgccgaag accacggccc agatcatctg
     7681 gctgtactgg aagggtgcca cgaccacagc cggcgcctcg cgcgtggcga agaccagccc
     7741 agcctgcccg gcgcccatca ggagacccgc catcgccatg gagccgagct cggccgggct
     7801 cggccagacc caggcgagga gcgccggcgg cagggccacc gccatcagcc agagcatcac
     7861 cgacatgagc atggcgccgc tcttctcggc cgccccgatc ttcttgagga tgatgaagct
     7921 gagcgcgccg gcgagcgccg cgagcccggc cagcgcatgg ccgaggccga gcgacgagaa
     7981 ttccgggtcg atcatgacga gcacgccggc gaagcccagc agcgtcgccg accagcggcg
     8041 ccagccgacc gattcgcgca ggagaatggc cgagaagccg gtgaccagca tcggcgccgc
     8101 gaagagcagc gcatagacct ccgccatcgg caggagggcg aaggcgctcc aggcgaggca
     8161 gccgcagatc gcggcgagaa tgccgcgcgc ggcgaccagc ggcttgttgc gcgggacgag
     8221 cgaccgcagg ccaccccggc cgatggtcgt ggccagcacc ggcagcatgg cgaagaaggc
     8281 catggtgaag gcgatctgga agaccgagaa ggcgcccgag aatgccttga gcagggcatc
     8341 ggccgtggag aacgaggcat aggccagaag cgacatgccg atgccccgcg cgacgcggct
     8401 cggctcgacg gcgacaatgg cgagcgtgct catggaggct gcaggtccgt ggttgaattg
     8461 cccggacctt atgccgaatt gccgggacac agagccagca gatcacacgc tccggacctg
     8521 ccgtttgcgc atggctcccc tgccggatcg gcgctgatgg cggggatgaa ttctcaactt
     8581 tttctcagat ggcgaaatcg atgccgtcct tgatctcgcg gcgcacggcc gactgctcgg
     8641 cggcatggca gagatcctcg atcgtgatgg catcgagccg ggcgagcatc gcctgctcgt
     8701 gctccagcca gaagggcgcc atgacccgct ggccgaggtc gctcgcggag agcgtgtcgg
     8761 gctcggcctc gccggactcg accacgttga cgatgtcggc gacggtgatg cggcgccgct
     8821 cgcgggccag ccggtagccg ccgcgcgggc cgcgcacgcc gcgcaggatg ccggccttca
     8881 ccagctgctg catcacctgc tcgagatagc ggcgcggcag gttcagccga cgcccgatat
     8941 cctggctctg caccggctcc gacgtgccgt gataggcgat gtcgacgacc gcctcgacgg
     9001 ccaacgccac cttcttcgac ggcttgatca tcgcgtctgg ctccccttcg tggctggttc
     9061 ggtcccgagg tggagcggcc ggctgccggt cgagccgaag ccgccggccg cccgatcgct
     9121 caccgagagg gcgctgacgg acagggtgcc ggtcgcctcg agccagcgtg cccgggccac
     9181 cggtgccagc acgagctgcg caatacgctc gccgcgccgc accctgaatg tctcctgacc
     9241 cagattgacg agcacgaccc cgatctcgcc ccgataatcg gcgtcgatcg tgcccggtgc
     9301 gttgaggacg gtcacgccgt gcttcagggc gaggccggag cgcggccgga cctgggcctc
     9361 gcagccttcc ggcagggcga tccggagccc cgtcggcacc agcgcacgct gccctggatc
     9421 cagggcaacg ggcccgtccg ccggcacggc ggcatggagg tcggccccgg cggcgagagc
     9481 tgtcgcatag gccggcagag gcagtccctc gccgtgcgcc aggcgctcga tcgtgacgtc
     9541 gatcctcatc ggccgagccg ctcggcgatg cgtgccacca gccgggtggc gagctcgtcc
     9601 ttgtccattt ccggccagga ctcgaccggt gcttcggaac cgagcagatg cacgcggttg
     9661 cgcgagccgc cgaagacccc actgccggtg ctgacgtcat tcgccaggat ccagtcgcag
     9721 cccttgcgct cgagcttggc cgccgcgttg gcgagaaggt cgtcggtctc ggcggcgaag
     9781 ccgaccacca gctcgggtcg catctgagga tgatgcccca accctctcag aatatccgga
     9841 ttctcgacga gctcgagggc ggcgggctgg tttcgcccct tcttcagctt cgcctcggca
     9901 gcctgggccg ggcgccagtc ggcgacggct gcggcgcaga ccgcgatgtc cgccggcagg
     9961 gcggcctcga ccgcatcgcg catcgctcgc gcactctcga cccgacgcac cgccacgccc
    10021 ggcgggtcgg gcaggctcac cggcccgctc accagcgtca cctcggcgcc cgcggcggcc
    10081 agggcggcgg cgatcgcgtg gccctggcgg cccgaggagc gattggcgat gtagcggacc
    10141 ggatcgatcg cctcgtgcgt cggcccgctg gtgacgatcg cccggcgacc ggccagcggc
    10201 cgctcgccgg gcggtaccag gatggcgtgg atcgccgcca ggatctcggc cggctccgtc
    10261 atgcgcccgc tgccgacctc gccgcaggcc atgtcgccgg ccgcgggccc gacgaagtgg
    10321 atgccgtcgg cccggagccg cgcgacgttc ctttgggtcg ccgggtgctg ccacatcatc
    10381 gggttcatgg cgggcgccac cagcacgggc ttgtcggtgg cgagcagggt ggtggaggcg
    10441 aggtcgtcgg cgtggccttg cgccatcttc gccatgaggt ccgcggtggc gggggccacg
    10501 accacgagat cggcctcgcg ggagagccgg atatggccga tctcggcctc gtcggtgagc
    10561 gagaagagat cgctgtagac ctttgattcc gaaaggctgg agacggccag aggggtgacg
    10621 aactcggccc cggcgcgggt caggatcgtg cggaaggcga ggccgcgctc gcgcccgcgc
    10681 cggatcagct ccagcgtctt gtaggcggcg atgccaccgc cgatgaccag gagaatgcgt
    10741 ttcgcattca tcggctttcc tgccctgcga ccgggtgctg tgggcgagcc tgcccgcgca
    10801 ccctctattt acgtgaatga agtgggatag cttcagccaa cggcaagccc gatgaggacg
    10861 ccgacgacga gcggccagaa ccagccctgg aacagcttca cccggtcgac cgcgacatgc
    10921 cgggtgcgtt cccgctcgag cgcctcgtcg agccgccgca cgatgtgcgg caggcggccc
    10981 gccatctgca ggccgtcgtc gagcgcctgc tgcaggcggg ccttcgggcc gagatgggcg
    11041 atgatccagt cctccaccag aggctgggcg agctgccaca tgttgacctt gggattgagc
    11101 agccggccga ccccctcggc caccaccatg gtcttctgca ggaggagaag ctgcggctgg
    11161 gtctgcatgc ggaactgctc cgccatcgcc aggagctggc ccatcaggcg gccgaacgag
    11221 atctcctcga gcggcagtcc caggatcggc tcgccgatcg agcgcaccgc ctggacgaag
    11281 gccatccggt cggcgtcatc gggcaagaag ccggcagcga agaagacgtc cgccaccttg
    11341 acgtagtcgc gctccaaaaa gcccacgagg atcccggcca tgtgccggcg gtcggccatg
    11401 tcgacccgcc ccatgatgcc gaaatcgagg gcgacgatgg tcccctcccg gtcgatcagg
    11461 atgttgccgg ggtgcatgtc gccgtggaag aagccgtcct tgaagacctg gctgaagaag
    11521 gccttcgccg cgttctccat gatccggtcg gggtcgtgcc cggcggcgat cagggcggca
    11581 cggtcggtga tgccctggcc gacgatccgc tcgaaggtga ccacccggcg gccggtccgc
    11641 cgccagtcca ccgccggcac gcgaaagccc tcgtagccct cggcgttctc ggcgagctcg
    11701 accgcggctg ccgcctcgag ccgcagatcg agctcgcgcc tggtcacctg ggcgaagatg
    11761 gcgaccgcct cgaccggctt gtaatggcgg agatgcggct gggtccgctc gaccagccgg
    11821 gccagccagg cgaagaaccc gagatcgcgc tcgatcgcct tctcgatgcc cggccgcagg
    11881 accttgaccg ccacctcgtc gccatcggtc gtcgtcgcca ggtgcacctg ggcgatcgag
    11941 gcggcggcca ccggccggtc ctggaaatcg ctgaagagcg cttcgaccgg ctgctcgaga
    12001 tcggtcgcga tggtcgcccg cgcggccgcg gccggaaagg acggcagcgc gtcctgcaag
    12061 gtggcgaggt cgcgggcaat ggcctcgccc aggaggtcgg tgcgggtggc gaggctctgg
    12121 ccgaacttga tgaaggacgg cccaagctcc tcgagcgctg cggcgagccg ttgcccgggc
    12181 cgcccggcgg cgttctcgtt gacgaacggt cgggcgagcc gcagcagggg ccgcgcctgc
    12241 ggcagcagct cgaagggaaa gagcgcgttg tgccgggcca gggtccggcc gatggcgacc
    12301 agccgccaga gatagcgcgg gctctcgagc atggcggggg ccggccgctt gcgcctagag
    12361 ccgccagccg gaatggatgg cggcgatgcc gcccgacagg ttgcgccagc gcacctgctc
    12421 gaagccggcg tcgcgcatga ggtcggcgaa ggtcggctgg tcgggaaagc ggcggatgct
    12481 ctcggcgaga tagcgatagc tcgccgcatc gccggcgacg atgcggccga gggcggggag
    12541 cacctggaag gaataggtgt cgtagacccg atcgaggacc ggtagcacca ccttcgagaa
    12601 ctcgaggcac atgaaccggc cgcccggacg gagcacgcgg aagccctcgg cgagggcccg
    12661 gtcgacatgg gtgacgttgc ggatgccgaa ggccatggtc acggcgtcga agcggccggc
    12721 ctcgaagggc agcgccatgg cgtccgcgca gacgaaggcg agacggtcga accagccgcg
    12781 cccgagcgcc cggtcccgcc cctggccgag catcgcggca ttgatgtcgg cgagcaccgc
    12841 ccggccgtcg ccgccgaccc gccgcagcac ccgctcggcc acgtcgccgg tgccgccggc
    12901 cagatcgagc aggtggatgc cggggcgcgg cgccagccag tcgagcaccg cctccttcca
    12961 cagccggtgc acgccgaagc tcatcagatc gttcatcagg tcgtagcggc cggcgacgct
    13021 gtcgaacacc tggcgcaccc ggccggcctt gactgccggt gccaccgcct cgaagccgaa
    13081 gctcgaggcg agctcggccg cggcagccgc cggcctgtcc gtcttctcca tcgatctcac
    13141 ttctcgtgcg gtgaacagcg gtgttgtagg aaagcccacc cggcgcggca accatccccg
    13201 cgatcaccac cgcaacagcg acggaatccc ttcttgcccg agctccccga ggtcgagacc
    13261 acgctcaggg ccctcgccca ccgcctcgac gcccggcgca tcaccggcgt cgagcagcgc
    13321 cgcgccgacc ttcgcttcgg cctgccggcc gggctgccgg cccgactcgc cgggcgaagg
    13381 ctcaccgggt tcgcccgccg ggccaagtac ctccagctca tgctcgacga cggcatggcg
    13441 atgctcctgc atctcggcat gtccgggcgg ctgctcttcg acgggccgcc cgccgggccg
    13501 cacgagcatc tcaccttcca tttcgacgac ggcacgatcc tcagattcgt cgacccccgc
    13561 cgtttcggca tgctcgacct cagcccccag gcgacgctcg gcgagcacaa gtggctggcg
    13621 catctcggcc tcgagccgct cggcaacgcc ttcgacggcg ccgcgctccg ggaccgcctc
    13681 gccggccggc aggtggcgct caagatcgcc atcatggacc agcgccacgt ggtcggcgtc
    13741 ggcaacatct acgccagcga gagcctgcat cgcgccggcc tctcgccccg ccgcctcgcc
    13801 tgcacgctcg gcccggcgcg cgcccagcgc ctcgccgcgt cgatccgcga ggtgctgctc
    13861 gaggccatcg acgccggcgg ctcgtccttg cgggactacg tccagtcgaa cggcgagctc
    13921 ggcaatttcc agcgccagtt ccgcgtctac gatcgcgccg gcctgccctg cacgggctgc
    13981 ggccgaccgg tcgcccggct ggtgcagggc gggcgcgcca ccttcttctg cggccgctgc
    14041 cagcgctgaa cggcttgatt cgccgccgcg agaatgcgtc ggtttaactg gacggacccg
    14101 gcaggcttgg tgttaaaggc ggcgcctgag atacgccccg cgggtgacgc cccggcccca
    14161 aaccctgtct tcagccaagc agctccccgt tcagaggcga gccacggaga actttcatgg
    14221 ctttcgagac gatccttgtc gacaagcgcg acacgatcgc cgtcatcacc ctcaaccggc
    14281 ccaagcagct caatgcgctg aacggccagc tcatcgacga gctgcgccgc gccctcgcca
    14341 gcctcgacgc cgacccggcc atcgccgcca tcgtgctcac cggctccgac aaggccttcg
    14401 ccgccggtgc cgacatcaag gagatggccg agctcgattt cgcccaggcc tatggcagcg
    14461 acttcatcgg cgcctggcac aggatcgccg agcaccggaa gccggtgatc gcggcggtcg
    14521 ccggctatgc gctgggcggc ggctgcgagc tcgccctgat gtgcgacatc atcctcgccg
    14581 ccgagaacgc caagttcggc cagcccgaga tcaatctcgg gacgatcccc ggcgcgggcg
    14641 gcacgcagcg cctgacccgc gccatcggca agtcgaaggc catggagatg atcctgaccg
    14701 gtcgcaacat ggacgccgtg gaggcggagc gggccaacct ggtggcgcgg gtggtgccgc
    14761 tcggcgacct gctcgacgag gcggtggccc tcgcccaggc catcgccacg aagtcgcagc
    14821 cgatcgtcgc catggcgaag gaggcggtgg acgtcgccta cgagacgacg ctcgccgagg
    14881 gcatccgctt cgagaagcgc agcttctacg ccaccttcgc caccgccgat cggcgcgagg
    14941 gcatgctcgc cttcgccgag aagcggagcc ccaacttcgc ccagagctga gccaccgccc
    15001 ccgacgagcg ggcagcgccg attgacgggc gcccgcttca tgggtatagg gacgggcctt
    15061 tttacagatg agggttatcg tggcgcatag tcgtacagcc aggaagcgta tccgtcagac
    15121 cgcgaagcgg acggagctca acaagagccg gatcagccgg attcggacat tcgtccgcaa
    15181 gctggacgac gcgctcgcac gcggcgacgc gccggccgcc acggccgcct tcgccgccgc
    15241 ggcgccggag ctgcaacgcg gcgtgaccaa gggcgtgctc catcgcaaca ccgtggcgcg
    15301 gaagatctcg cggctgtcgc agcgtgtccg cctgctggcg accagccagc ccggttgaat
    15361 catcgggcgg gtcgtcccgc cccctccatc accgacgacg tggagcaccg agcaagcgtg
    15421 gccctagagc tatggggcga cgctccctcg gtgttgacca acacgcccga ttcatcaact
    15481 aaaacgcgaa tttctgcgtt ctcttttcga tcctcttgcc agcgcgttca tgcacggtta
    15541 ctgtccggag atgcgctgcg gcttcccgcc tgcgtcgatc atcatcgaac tacagtaacg
    15601 cgtacagtca agcagagtat gtgggcgcac tctattgatg ctaaaccgac aggcgagcaa
    15661 cgacagggcg agcaggcgcc aagaaccgtc atgcgcttgt cgtggtctcg tcatcatggg
    15721 ctcggcgcgt tgctggacgg ggcacgacag ggcaagtaac caaggcgaac ggcagtcccg
    15781 ccggttcgct ccgcactaga acggatcgca cgctcatggg tcgagggagc aagtccgagg
    15841 cggttgtcga ggcagatatg gccggtccgc ttggcgctga actgacgcag caatggtcgc
    15901 gcgtgcgcgg cctgctccgg gccgagttcg gcgacaccgc ctactcgacc tggctgcgcc
    15961 cgctcgaggc catcgccctc gacggcgacc gcgtgctgat agtcgtgccg acccagttca
    16021 tgcgcgactg ggtggtcaac aactacgccg accggatccg cgcccactgg accagcgtca
    16081 acccgacggt ccgcagcgtg gcgctcgacg tgcggccggc gcgactcgac ccgacgcgac
    16141 tcgaccctgc gcggctcgac gacagcggcc gcctggccgg acggcctgcg accaagccgc
    16201 gcatcgtcgc cgacaatgtc cgcgaggccc atgctgaagg cgcgcccccg gcagtggaat
    16261 cccgcgagcc gggcaaggca gccgaccggc aggccggacc ggcggagctc gccgagttcg
    16321 gcgccgggct cgacccgcgc ttcaccttcg acaccttcat cgtcggcaag cccaacgagt
    16381 tcgcggtggc cgccgccgag cgcatggcgc tgagcgccac gccgcccttc aacccgcttt
    16441 tcctctatgg cggggtgggg ctcggcaaga cccatctcat gcacgcgatc ggctggcggg
    16501 cgaagcagaa cgatcccgac cgccgggttg tctacctttc ggccgagaag ttcatgtacc
    16561 agttcgtccg cgccatccgg cagcgcgaca ccatgggctt caaggagatg ttccgctcgg
    16621 tcgacctcct catggtcgac gacgtgcagt tcatcagcgg caaggacagc acgcaggagg
    16681 agttcttcca caccttcaac gcgctggccg aacgtggcag ctccattgtg atttcagccg
    16741 atcgcagccc ctcggacctc gccgggctgg aggagcgcat ccgctcgcgc ctcggctggg
    16801 ggctggtcgc cgacatccac cccaccacct acgagctccg cctcggcatc ctccaggcca
    16861 aggccgagca gctcggcgtc gaggtcgagc cggaggtcac cgagttcctg gcgcaccgca
    16921 tcacctccaa cgtccgcgag ctcgagggtg cgctcaaccg catcgtccac caggcgacgc
    16981 tgatccgccg gccggtctcg atcgagatgg cccaggacgt gctccgcgac ctcttgcgct
    17041 cgaacgagcg gcgggtgacg atcgaggaga tccagcgggc cgtggtcgag catttccagc
    17101 tcaagatggc cgacatgagc tcgacccgcc gggcgcgggc cgtcgcccgg ccgcgccagg
    17161 tggcgatgta cctcgccaag cagctcaccc cccgctcgct gcccgagatc ggcaagaaat
    17221 tcggcaatcg cgaccacacc acggtgatgc acgcggtcaa gcagatcgag aagctctgcg
    17281 aggaggaccg ctcccttgcc gacgacgtgg cggcgctgaa gcgccggctc gccggctgag
    17341 ccggcccggc cggggccgcc ccggcccccg gccccgctga tgacgaaaaa gatcaacgcg
    17401 cgcgcggaat tctgctagct tctccggccc ataggacggg tgcggagacg cctgcttctc
    17461 ttcgcccaaa tcgagagaat tcccgttcca atgaagctag cgattgagcg tgctgccctt
    17521 ctgcgatcgc tgtcgcacgt ccaggccgtc gtcgagcgcc gcaccaccat cccgatcctg
    17581 tcgaacgtca agctcgcggc aggtggcgat ggcctgcatc tgacggcgac cgacatggac
    17641 ctgtccatgg cgtcgagcga gacggcgcgg gtgcaggacg atggcgtcac caccgtctcg
    17701 gcgcacacgc tcttcgacat cgtccggaag ctgcccgatg gcagcgaggt gctgatcgag
    17761 caggagccga acgcgagcga gatttcggtc cgcgccggcc gggcggtctt caccctgccc
    17821 tgcatcaagg ccgacgagtt cccggcgatg gacggcgacg ccgaggccta cgacgttacc
    17881 ttcgacatgc cggccgccga cctgcgcaag ctgatcgaca agacccgctt cgccatctcg
    17941 accgaggaaa cccgctacta cctgaacggc atccacgtcc atgcctcggg cgccggcgcc
    18001 acggcggtgc tgcgcggcgt cgccaccgac gggcaccgcc tcgccaagat cgagatcgcc
    18061 ctgccggacg gggccgaggg catcccctcg atcatcatcc cgcgcaagac cgtgaccgag
    18121 cttcgaaagc tgatcgacgc catcgacggc gagatccggg tcaaggtctc gaccaaccgc
    18181 atccagttcg tcctcgacca ggcggtgctc gtctcgcggc tgatcgacgg cacgttcccg
    18241 gactacgagc gggtcatccc ggtcggcaac gagaaggtgg cggcgatcga cacgaagctc
    18301 ttcgccgaga ccgtcgatcg cgtctcgacc atctcgaccg agcgggcccg cgccatcaag
    18361 ctgcgcttcg acgacggccg gatcaccgcc tcggcggtca gcaccgaggc cgggcgggcg
    18421 gtcgaggagc tcgatgccga ctatgcgggc gagcctttgg agatcggctt caacagccgc
    18481 tacatcctcg acatgctggc cgaggtggcg ggtgaccagg tgcgcctcga gatggccaac
    18541 cccgccgccc ccacgctggt gcgcgacccg gccgacggca gtgtcgtcta cgtcctgatg
    18601 ccgatgcgtg tctgaccggc ggacccgacc gatggcggtc gcaggcgatg gcgagcgcga
    18661 ggcgaggttc agttgagccc gcttcgcgag gccgcgcgcg cgaggccgac ccgcgagacc
    18721 gatccggcga ggccggcgct ggagcgcctg cggctccggg atttccgcaa ctacgctggg
    18781 ctcgagctcg agctggcacc cggtccgatc gtgctcttcg gcccgaacgg cgccggcaag
    18841 acgaatctgc tcgaagccat ctcgctcctg gccccgggcc gcggcctgcg gcgcgcccgg
    18901 ctcgaggcgc tcgaccgcga tggtgccgcg gcccccttcc gcctcgaggc cgggctgcgc
    18961 acgcccgatg gccccgtcga cgtcgccagc ggccgcgagc cggagagcga ccgccgctgg
    19021 ctcgagatcg ccggccagcc ggtccgcggc ctcgccgccc tcgccgacct cgtcgccatc
    19081 acctggctca ccccggccat ggaccgcctc ttcgtcgacg ccgcggccga gcggcgccgt
    19141 ttcctcgacc ggctggtgct cggcgtctac cccgaccatg cgcgtcgcct cgccatcttc
    19201 gagcgggcga tgcgcgagcg ctcgctggtg ctgcgccagg gcccccgcga tgcgacctgg
    19261 ctcgacatcc tggaacagcg catggccgaa tcgggcgtgg ccatcgcggc gggccggcgc
    19321 gagctggccg ccggcctcgg cacgctcctg gccgagccgg acccggcttt gccccgtctg
    19381 cgcctcgagc tcgacggcag cgtcgagacc tggctcgacg acatggccgc cctcgacgcc
    19441 gagcagcgcc tcgccgacgc cctggccgcg gcccgcgccc aggatacgga ggccggtggc
    19501 gccgccatcg gcccgcatcg cagcgacctc gtcgccatcg atctcgccac caacgaggcg
    19561 gcgccgcgcg tctcgaccgg ccggcagaag gccatgctgc tcgccatcgt gctcgccgag
    19621 gtccgcctgc gcgagctggt cgcgggcgac ctgcccattc tcctgctcga cgagaccgcc
    19681 gcgcatctcg acccgacccg ccgtgccggc ctcgccgagc ggctcctcgc cctcggtgcc
    19741 caggtgctcc ttaccggcac cgagcgccat ctcttcagtg gcctcgacgg ccacgccacc
    19801 ttcatccatg tcgaccgagg actgatccga gccgatgagt gacacccccg agctgccgac
    19861 cgagccccag accggccgga ccggcgccgc caccgcctct gccactggcg gcgagccgat
    19921 ctacgattcc acctcgatcc gcgtgctcga aggcctcgag gcggtgcggc tgcgacccgg
    19981 catgtatatc ggcgacaccg atgacggctc cggcctgcac cacatggtct acgaggtggt
    20041 cgacaacgcc atcgacgagg cgctgg
//
LOCUS       DAOTFI010000069        19940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3267_length_19940_cov_6.565703, whole genome shotgun sequence.
ACCESSION   DAOTFI010000069 DAOTFI010000000
VERSION     DAOTFI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19940
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3267_length_19940_cov_6.565703"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="P5558_08890"
     CDS             complement(<1..930)
                     /locus_tag="P5558_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24463.1"
                     /translation="MKRLFARRPELRLAGGLFIVLLLLNLILNPVRFAPVNLGVTLGL
                     AAPLIIAAIASMPPILGGRGGIDISVAPLMGLVNVVIVRVLVERAGMTSPFEIVPIVL
                     LIGLASGVLNGLLATILRIQPIVATLGTYLLYAGLTLTISPSPTGTAPAWLKSLAGTG
                     SILPILAVVAVWLLIKRLPIHEHLMSVGSDDRAAFTAGIPVTRVRFLSYVVAGLFGAV
                     GGLSLTGLIGSADPNVANSMTLLAISAVALGGVSLAGGKGGLVAAALGAVDIFLLQSV
                     LTYFNVSTFLLQMAYGAALTVAVRFRGEAERLVD"
     gene            complement(927..1682)
                     /locus_tag="P5558_08895"
     CDS             complement(927..1682)
                     /locus_tag="P5558_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24464.1"
                     /translation="MAEGIMLEARGVTLEEAARPIDLAVARGEIVGLAGLEGHGQEAF
                     IETLCGLHRPVDGTVTIRTEVGREAPVTSLAEAVRLGVAYLPRDRRGTGIFPGQSVFD
                     NFAIAALPRHATLGVVRRRGLLQRLRDFRERLSMVYDRPDAPIGTLSGGNQQKVLLAR
                     WLACEPRVMLLNDPTRGVDLGTRMKLYDTFRGLARNEGMPLVVLSTEIEEILQLCDRV
                     LVFRERAVSRELGPDELAMDRVIAAMFGQGEAA"
     gene            complement(1675..2373)
                     /locus_tag="P5558_08900"
     CDS             complement(1675..2373)
                     /locus_tag="P5558_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24465.1"
                     /translation="MALDDVSVAFTAGQVHTILGENGSGKSTLVKLLTGVVEPNTGTV
                     EVDGQPVRLRNPAQARGHGIATVFQEVLLAPERSVLDNIFLGYDGLWRRALPLARRAG
                     IARATLARITRTALDLDRRGADLPLAQQQLVVLARALVRDPRILILDEATAALDFADR
                     DVLFAAIRELVAGGRLVLFISHRMDEVQALSDRVTVLRSGRAIETLEGAEITPARLLA
                     LMIPEAHLKVHAHG"
     gene            2561..3736
                     /locus_tag="P5558_08905"
     CDS             2561..3736
                     /locus_tag="P5558_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY24466.1"
                     /translation="MNIALRTLLCGLTLPALAALPAAAQSIDVLPDELKALYTDNIPV
                     GPSAYDGFEMPAQPWKWCHSESYMGNPWRVSMTNELERLVNGAIEAGVVESFELSDSN
                     GDVTQQIAQIRAFIDKGCSVITTIAGSSTALNDVIEDAFNAGIPVVTTASAVTSPYAF
                     NIQHNHNVWGYDMGIGVADYLGGPGNVLMVEGIPGSPIVEHENSGGYRAFEEHPEITI
                     LGKVSGMWTANVTKNVVLQFLATNASPIDAVWTTGSETRVIAEAFTEAGRDQPLITGS
                     MSGDALGYWKENKDTFKYYGGAILPTVAAHNTFRAALRLLEGQEPLVNTILVPVPEIR
                     GEDLGKWAAECMTVESGSIFPVAPVEPLSEESMNGYFRNGAATPLYDYATTPDPCAG"
     gene            3756..4874
                     /locus_tag="P5558_08910"
     CDS             3756..4874
                     /locus_tag="P5558_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRY24467.1"
                     /translation="MDQRLERTVQALREVGADWAVLASVDGVAYATGHFAPVEAGPSP
                     FAGGPTIALIDRNGTAGIACANVEAAAAGASRVTEVELYEGYAFDHGVDYIDSYRTAV
                     GSLARRLGVGGRLAGERGHVPQILGDVLPVDTALDLTPPLRRLRATKTAEELAALRRS
                     AEAVAIGQRRFLEALRPGRSELAVFAEIRCAIEEFAGCRVPITGDFISGAARSSAFTG
                     WPIHRVIEPGDPVMADLAPRIDGYWGDSCATTSAGPASKGFMKLFEASKGALTHALEI
                     IRPGMTAQELDAQLRAYVGRHGFTYPHHSGHSIGTAVHEFPRLVPYETAVLEPDMVLL
                     VEPGCYDPEIGGVRSEYMIRVTDSGCEILHPFDHVPSA"
     gene            4965..6344
                     /locus_tag="P5558_08915"
     CDS             4965..6344
                     /locus_tag="P5558_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRY24468.1"
                     /translation="MSARSLPPEGPLVAWYGDDFTGSAAVMEVLTFAGLPSVLFLEPP
                     TPAQLARFEGLRGIGIASTARAQGPRWMDANLPALFLTLRGLGAPINHYKVCSTLDSA
                     PEVGSIGRAIDLAIPVFGSDWVPILVAAPPLRRYQAFGQLFASGPGGVFRLDRHPVMA
                     RHPVTPMDEADVARHLARQTDRQLGLIGLEDLATADRALEARRRQVAEGAALLTLDCI
                     DEATLGMCGRLIWEARGPHQLVVGSQGIEYALIRHWQDCGLLPRHAPPAGIGPAKRMA
                     VVSGSVSPVTAAQIDWSLANGFAGIAFDARVVVDKAALAREEEAAVAAALDAIDRSLD
                     PLVFTARGPDDPAVPALREALGRSGLAAETANERIGTALGRIIDRIIRQAGLRRAVIS
                     GGDTSGHASRQLGIHALTALAPTLPGAALLRAHSDGAHDGLELALKGGQMGSPDYFGW
                     IRDGGGARG"
     gene            complement(6856..8103)
                     /locus_tag="P5558_08920"
     CDS             complement(6856..8103)
                     /locus_tag="P5558_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HRY24469.1"
                     /translation="MPAAEIRVTYRLETAGDPEATAAKIASDQSTGTFTELPGETAAV
                     RARCAARVVGLTPLEPLATPSIPDVGGRGPYKRADAVIAYPLEAIGTDVAALMTIAIG
                     GVYATKGLTGIRVMDIDLPDAWRCHPGPQFGIAGSRRLTGVESGPIIASIIKPSLGLL
                     PEETAEVVRTLCEAGVDFIKDDEKLMSPGYSPLEARVAAIMPVILDHEQKTGRKVMYA
                     FGISSADPDLMLRNHDLVLDAGGNAAVVNINSIGHGGMTFLRRRSALCLHAHRNGWDI
                     LTRHPGLGMEFRPYQKIWRLLGIDQLQINGIRAKYWEPDESFVRSFHDCMTPIFSEAD
                     RPLPVVCSGQWGGQAVETFERTGRTLDLMYLGGGGIHGHPMGPAAGVAATRQAWDAAA
                     AGISLEQQAQAHPELAAAIARFG"
     gene            complement(8126..8581)
                     /locus_tag="P5558_08925"
     CDS             complement(8126..8581)
                     /locus_tag="P5558_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRY24470.1"
                     /translation="MRIAVAGDSVGAELAHVLAEHLQGRHEVAELSTPQNDSEKLYAH
                     LVDRVASQVRDGLYDRGILVCGTGIGVCISANKVAGIRAALTHDTYSAERAALSNNAH
                     IITMGARVIGPELAKAIADAFLAQTFDEAGRSAPNVRAIDEVEAKYAKA"
     gene            complement(8691..9299)
                     /locus_tag="P5558_08930"
     CDS             complement(8691..9299)
                     /locus_tag="P5558_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRY24471.1"
                     /translation="MADWISGLVESGGYLAIVALVFLENVFPPIPSELILPLAGSVAG
                     RGGISLVGAVVAATIGSVAGALGWYWVGWWLGLERLKSWAAKHGRWITLSPEEIERAD
                     AWFDRHSGKSVLIGRLIPTIRTLISVPAGISGMTLRRFLLFSTLGTAAWSALLIGAGY
                     LLGEQYESVGNWLNPVSNLVAAGLGLWYAYRVLTFDRRLRSS"
     gene            complement(9321..9521)
                     /locus_tag="P5558_08935"
     CDS             complement(9321..9521)
                     /locus_tag="P5558_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HRY24472.1"
                     /translation="MERIMPAKSKAQQKAAGAALSAERGETKKSELQGASRSMVESMS
                     EEELERLASTRRKGKPEHVSKG"
     gene            9906..10778
                     /locus_tag="P5558_08940"
     CDS             9906..10778
                     /locus_tag="P5558_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRY24473.1"
                     /translation="MGLLRVMVVGGAVIAALVGGVAVHAARSNRENQEKLGRALIIGG
                     GVVVPAFALAALLVWSLPMMTAFRAPGDGLRIEVSGEQWWWRVRYWPEGAETPIETAN
                     LIVLPAGERTEIVLDSPDVIHSFWIPALAGKVDMIPGRRTRLVVEPQEPAELRGQCAE
                     FCGTSHALMSFPVEVVPAAAFAGWLEREAAHAAAAEPGPAEAGAELFSALGCGGCHNV
                     RGTGADGVIGPDLTHFASRPSIGAGTLPNEPEALRLWLATTGKVKPDVMMPSFGMLGA
                     PELDALIAYLAGLR"
     gene            10775..13297
                     /gene="ctaD"
                     /locus_tag="P5558_08945"
     CDS             10775..13297
                     /gene="ctaD"
                     /locus_tag="P5558_08945"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRY24474.1"
                     /translation="MSGEHDAADRAHREAQAERLLAAWRTPTGWRYWSAVNNSEVGAW
                     YTVAAFVFFLFAGVLALLVRVQLAVPSNDFLSADVYNQAFTLHGTVMMFLFAVPIFEA
                     FSILILPQILASRDLPFPRLSAYGFWSFVIGGVFVCGSIFFGAAPDAGWFMYPPLTTD
                     LEQSGIGTDIWLLGLSFIEVASIAAAIELIVGVLKCRPPGMRINFMPLYAWYVLVVGG
                     MILFAFPPLIAGDLLFEMERAFDWPFFDPERGGDPLLWQHLFWIFGHPEVYIIFLPAI
                     ALVAMIVPTFAQRPIFGYSWVVLAAVGTGFLSFGLWVHHMYATGLPAISIGFFSAASE
                     AVVIPTGVQIFVFIATLLTGRVIFSTPLLFIAGALAIFLIGGLTGVMVALAPFDWQAH
                     DTYFVVAHLHYVLIGGMLFPIVAGFYYYFPLVTGKKLSDRLGRTAFWLMFSGFNIAFL
                     PMHLTGLRGMPRRVFTYPEGLGWDTLNMVSTVGAFIFAAGILVVLLDVIRPKRRQPYA
                     ERNPWNAGTLEWLCEMPGKPWGVRSIPLVTSRYPLWEQANFVRDVDEGRFFLPDAEEG
                     ERETLVTSVLDAEGVQCLRVPGPTFLTMAAAVFTGGVFIFATYHWYAATAVSGVLALA
                     SIIAWLWTGTAVIPEKDEKHIGHGRTLPLYMSGPRSVGWWAMCITMIGDMTAFFSLVF
                     TYFFFWTVNPSFPPEAAPGPGVLWPAVALAMLIGAWALTTMARRWNARDRVLSLRAGL
                     ALAIVLAAASMGALVFGVRGTGLDPTASSYGATVWVLILWLAAHVAVGSVMQAYCIAR
                     SMAGKLTGKWDIEIRNVSLYWHFLAFTAAATTAVIALFPLVD"
     gene            13344..13682
                     /locus_tag="P5558_08950"
     CDS             13344..13682
                     /locus_tag="P5558_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24475.1"
                     /translation="MTGPVVWAVHFLVCYIGTAIFCARAGDPEADLAPVRLGIGAITL
                     VALAVIAFVGIEADRRSGPGLVFECPHDEDTVRERRQFLGYATLLLAGVSFIATLLVA
                     LPALVIPSCR"
     gene            13679..14326
                     /locus_tag="P5558_08955"
     CDS             13679..14326
                     /locus_tag="P5558_08955"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRY24476.1"
                     /translation="MSRLGAAAVGGLLLVAAWAGPLAAHAASSFTAHMAIHVTVIAIA
                     APLLAFAMGPRFALARRCPLLFAPLVASLLDIVAIWGWHAPAPHVLARTSALGFALEQ
                     LSFLAVALLVWTSALGAGQSESRAGQAAGVGALLFTSMHMTLLGALFALAPRELFPRE
                     ICGSAFAGLTALQDQQLGGTLMLALGGASYLAGALYLLARLLSDGALGARDARHG"
     gene            14310..15386
                     /locus_tag="P5558_08960"
     CDS             14310..15386
                     /locus_tag="P5558_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRY24477.1"
                     /translation="MRVTARGLALGLAGLLAAGALFAWSGLLNVSASSGHWAITSWFL
                     HAVMRQSVETHAGGIEAPDLDDPAMVLRGAGHFATGCAYCHGAPGKPPSPAVRNMTPA
                     PPRLGGTLREWEPAELFWIVRHGIKFTGMPAWPAPERADEVWSVVAFLRALPGMAPET
                     YRRLAHGPASAEGRADAGAALTSCARCHGFDGSGRGAGAFPVLSGQNEAYLLASLRAF
                     ASGSRASGIMETAVNALDDEALSDLARHYARQPRPERPAAPPPAAALAAEGERIFNEG
                     LPRQRVPACASCHGRRGDAPRYPSYPRLAGQPRHYLETQLELFAAGSRGGTSFSHVMT
                     MVAGRLEPAQIEAVAAWLASLPPQ"
     gene            15452..16276
                     /locus_tag="P5558_08965"
     CDS             15452..16276
                     /locus_tag="P5558_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRY24478.1"
                     /translation="MAPRAIWKGQLQISELVCPVSLHAAASTAGRVSFHLVNRATGNR
                     VRREYVDAETGEQVEREQQVKGYATDDRRYIPIEPEEYAAALPENHKTLQVEAFLDCA
                     DIDTVYLDRPYFLLPAEPTAEEAFTVIREGLRARRKAAVARAMLFRRVRSLLIRAQGR
                     GLIASTLRFDYEVRPAEEAFAGIHEQAIQGEMLDLARHIIASKRGEFDPAAYDDRYDA
                     ALAGLVKAKAAGRKIRPRGRRPEGRVIDLMDALRKSAEGAGPASRREAASRNRKAG"
     gene            16279..18786
                     /gene="ligD"
                     /locus_tag="P5558_08970"
     CDS             16279..18786
                     /gene="ligD"
                     /locus_tag="P5558_08970"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HRY24479.1"
                     /translation="MALDTYRERRDFRKSPEPRGRPGRRRTARKGRAFVVQMHAARRL
                     HYDLRLEMDGVLKSWAVTRGPSLVPGEKRLAVHVEDHPLEYGDFEGTIPAGSYGAGTV
                     IVWDSGSWQPIGDAAKGYAKGHLDFELAGEKLEGRWHLVRMAAKPREKRENWLLIKGD
                     DEHARGADDPDILEERPESTKTGRRLEEVAESAPKKEKRARPAGKRSSRPLGDARQAP
                     FPGFVAPALATLRPEAPRGERWVHEIKLDGYRLQAHVQGGEARLLARSGADWTERFGE
                     AIAAALAALPARDAVIDGEVVVEARNGSSDFAALQQDLSEERTDRFVYYAFDLLYLDG
                     YDLRPGALEDRKARLEHLLDGSPPALRYNDHFDEEGELVLRHACRLSLEGIVSKERQA
                     RYASGRSTSWIKSKCRERQELVVAGYVPSSTLPKSVGSLVLACHEGGKLVPAGRVGTG
                     FSRRMAAELYRRLAPLERAKPPFSGSPSAIDAADRRQVRHVEPELVVEVEFQSWTAGG
                     LLRHASFRGIREDKAPGEIVREGEPKRGAGDRAPRAVRLTHPDRLYWPEAGVTKEGLA
                     DYYAEVWPWMAAFVVGRPLALLRCPQGATRACFFQKHAWKGQSREILKALDPLDESGD
                     ASMVAIDGLDGLIGLVQGGALELHGWQSSLDALEQPDQIVMDLDPGDDVAWDAVIAAA
                     GEVRDRLAEAGLQSFVKTSGGKGLHVTAPLRPKAGWDEVKAFARSIADAMAADHPDRY
                     VATVAKAKRRGRILIDYLRNGRGATAVVPCSTRARAGAPVAMPLGWDELGPAIGPAHF
                     TVLDAAGRLRNLARDPWGEFRKAAATLPPARSPRRRR"
     gene            18683..19366
                     /locus_tag="P5558_08975"
     CDS             18683..19366
                     /locus_tag="P5558_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRY24480.1"
                     /translation="MPPGGCETSLATPGASSARPRRRCRRQGRQGVAAEPQRRIFPTW
                     PCNSSTDGPRHRRVGASRDALAARALAFTILTAARSGEVRGLRWGEIDTAQAVWTVPG
                     ERMKAGLEHRVPLVPEAVACLPPRDDDPAALVFPGAIDAERPLSDMTLLAVLKRMGRS
                     DLTVHGFRSTFRDWAGESTTHAREVIEAALAHRLKDKAEAAYARGDLFAKRRLLMQDW
                     AHFALSAAA"
     gene            19420..>19940
                     /locus_tag="P5558_08980"
     CDS             19420..>19940
                     /locus_tag="P5558_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24481.1"
                     /translation="MIDRSSPADAHQPSSNPLTCTGALKIVRLERPEDLHEQLAKAIQ
                     AELTEAQVDALLKVLEDYVNRGLRAARVPEVGGLRRRLNKLARLTDQLARLLIEIEQK
                     GEGDWYALTRVIGHQELSTYCVGTGLIKQVAYKTESDLVAVDRRAWASFEGLVHQLAD
                     IFEQTGRRATAQCT"
BASE COUNT         2887 a         7007 c         6967 g         3079 t
ORIGIN      
        1 atcgaccagc cgctccgcct cgccccgaaa ccgcacggcg acggtgagcg ccgcgccata
       61 ggccatctgc aggaggaagg tcgagacgtt gaagtaggta agcacggact gcagcaggaa
      121 gatgtcgacc gcacccagcg ccgcggcgac gaggccgccc ttgccgccgg cgaggctgac
      181 gccgccgagg gccacggccg agatggcgag gagcgtcatg ctgttcgcga cgttcgggtc
      241 ggccgagccg atcaggccgg tgagcgagag cccgccgacg gcgccgaaga ggccggccac
      301 gacataggag aggaagcgaa ccctggtgac cgggatgccg gcggtgaagg cggcgcgatc
      361 gtcgctgccg accgacatca aatgctcgtg gatcggcagg cgcttgatca gcagccagac
      421 cgccacgacg gcgaggatcg gcaggatcga gcccgtgccg gcgagcgact tcagccaggc
      481 cggtgccgtg ccggtcggcg agggcgagat cgtcagcgtc aggcccgcat agaggagata
      541 ggtgccgagc gtcgccacga tcggctggat tcgcaggatg gtggcgagaa ggccgttgag
      601 caccccggat gcgagcccga tcagcaggac gatgggcacg atctcgaagg gcgaggtcat
      661 gcctgcccgc tcgaccagca cgcggacgat cacgacgttg acgaggccca tgagcggcgc
      721 caccgagatg tcgataccgc cgcgcccgcc gaggatgggc ggcatcgagg cgatggcggc
      781 gatgatcaaa ggtgccgcca ggccgagcgt cacaccgaga ttgaccggcg cgaagcgcac
      841 cgggttgagg atcaggttca agagcaggag cacgatgaac aggccgccgg cgaggcgaag
      901 ctcggggcgg cgggcgaaga ggcgcttcat gcggcctccc cttggccgaa catggcggcg
      961 atcacccgat ccatcgccag ctcgtcaggg ccgagctcgc gcgagaccgc gcgctcgcgg
     1021 aacaccagca cgcgatcgca gagctgcagg atctcctcga tctcggtcga gagcaccacg
     1081 agcggcatgc cctcgttgcg ggcaagcccg cgaaacgtgt cgtagagctt catcctcgtg
     1141 ccgagatcga ccccgcgcgt cgggtcgttc agcagcatca cccggggctc gcaggcgagc
     1201 cagcgcgcca gcagcacctt ctgctggttg ccgcccgaga gcgtgccgat cggcgcatcg
     1261 ggccggtcgt agaccatgga gagccgctcg cggaagtccc gcagccgctg gagcaggccg
     1321 cgccggcgga ccacgcccag cgtggcgtgg cgcggcaggg cggcgatggc gaaattgtcg
     1381 aacaccgact ggccgggaaa gattccggtg ccgcgccggt cgcggggcag gtaggcgacg
     1441 cccagccgga ccgcctcggc gaggctggtg accggcgcct cgcggccgac ctcggtgcgg
     1501 atcgtcacgg tcccgtcgac cggccggtgc aggccgcaga gcgtctcgat gaacgcttcc
     1561 tggccgtgcc cctcgagccc ggcgagcccg acgatctcgc cgcgcgccac ggcgagatcg
     1621 atgggccggg cggcctcctc cagcgtgacg ccgcgcgcct cgagcatgat gccctcagcc
     1681 atgcgcgtgc accttcagat gggcctctgg gatcatcagg gcgagcagcc gggcgggcgt
     1741 gatctccgcg ccctcgaggg tctcgatggc ccggccgctg cgcagcacgg tgacccggtc
     1801 cgagagcgcc tgcacctcgt ccatgcggtg cgagatgaag aggacgagcc gcccgcccgc
     1861 caccagctcg cggatggcgg cgaaaagcac gtcgcgatcg gcgaagtcga gggcggccgt
     1921 cgcctcgtcg aggatcagga tgcgcgggtc gcgcaccagc gcacgggcga gcacgacgag
     1981 ctgctgctgg gcgagcggca gatcggcgcc gcgccggtcg agatcgaggg cggtgcgggt
     2041 gatccgcgcc agcgtcgccc gggcgatccc ggcgcgccgc gccaacggca gagcccgccg
     2101 ccagagcccg tcatagccga ggaagatgtt gtcgagcacg ctgcgctcgg gggcgagcag
     2161 cacctcctgg aacaccgtgg cgatgccgtg gccgcgcgcc tgcgccgggt tgcgcagccg
     2221 caccggctgg ccatcgacct cgacggtgcc cgtattgggc tccaccaccc cggtcagcag
     2281 cttgaccagc gtgctcttgc cgctgccgtt ctcgcctagg atggtgtgga cctgcccggc
     2341 ggtgaaggcg accgaaacgt cgtccagcgc caccgtctcg ccgtagcgtt tcgagacccc
     2401 gttgagcgcc agaatggtca gccgactttc cccgattgcc gaacccggag cctgcccgat
     2461 cggtcggctt tccgggcgaa atcgctagac ggtccgggac attcatgcaa gacacggcgg
     2521 cggcgaattc gcctgattgc tcggggagga gctgcaagaa atgaacatcg ccctcaggac
     2581 gctcttgtgt gggctgacgc tgccggcgct ggcggcgctg cccgccgcgg cccagtcgat
     2641 cgacgtcctg ccggacgagc tcaaggcgct ctacacggac aatatcccgg tcggcccctc
     2701 ggcctatgac ggcttcgaga tgccggccca gccctggaag tggtgccact ccgaatccta
     2761 catgggcaat ccctggcgcg tctcgatgac caacgagctc gagcggctgg tcaatggcgc
     2821 catcgaggcg ggtgtcgtcg agagcttcga gctctccgac agcaatggcg acgtcaccca
     2881 gcagatcgcc cagatccgcg ccttcatcga caagggctgc agcgtcatca ccaccatcgc
     2941 cggctcgtcg acggcgctca acgacgtgat cgaggatgcc ttcaacgccg gcatcccggt
     3001 ggtcaccacc gccagtgcgg tgaccagccc ctatgccttc aacatccagc acaaccacaa
     3061 tgtctggggc tacgacatgg ggatcggcgt cgccgactat ctcggcggcc cgggcaacgt
     3121 gctgatggtc gagggcatcc ccggcagccc gatcgtcgag cacgagaact ccggcggcta
     3181 ccgcgccttc gaggagcatc ccgagatcac gatcctcggc aaggtcagcg gcatgtggac
     3241 cgcgaacgtg accaagaacg tcgtgctgca gttcctcgcg accaacgcct cgccgatcga
     3301 cgccgtctgg acgaccggca gcgagacccg ggtgatcgcc gaggccttca ccgaggccgg
     3361 ccgcgaccag ccgctgatca ccggctccat gtccggcgac gcgctcggct actggaagga
     3421 gaacaaggac accttcaagt actatggtgg cgccatcctg ccgacagtcg ccgcgcacaa
     3481 caccttccgc gccgcgcttc gcctgctcga gggccaggag cccctggtca acaccatcct
     3541 ggtgccggtg cccgagatca ggggcgagga cctggggaag tgggcggccg agtgcatgac
     3601 cgtcgagtcc ggctccatct tcccggtggc gccggtcgag ccattgagcg aggagtcgat
     3661 gaacggctac ttccgcaatg gtgccgcgac accgctctac gactacgcca ccacccccga
     3721 tccctgcgcc ggctgaacgg cgcgaggatc gcagcatgga ccagcgtctc gagcgcaccg
     3781 tgcaggcgct gcgcgaggtc ggcgccgact gggccgtcct cgccagcgtc gacggcgtgg
     3841 cctacgcgac cgggcacttc gccccggtcg aggccggccc ctcccccttc gccggcgggc
     3901 cgaccatcgc cctgatcgac aggaacggca cggccggcat cgcctgcgcc aacgtcgaag
     3961 cggcggcggc cggggcgtcc cgggtcaccg aggtggagct ctacgagggc tacgccttcg
     4021 accatggcgt cgactacatc gacagctacc ggaccgccgt cggctcgctc gcgcgacgcc
     4081 tcggcgtcgg cggcaggctc gccggcgagc ggggtcatgt cccgcagatc ctgggcgacg
     4141 tcctgccggt cgacacggcg ctcgacctca cgccgccgct ccgccggctc agggcgacca
     4201 agaccgcgga agagctggcg gcgctgcgcc ggtccgccga ggccgtcgcc atcggccagc
     4261 ggcgcttcct cgaggcgctg cgtcccggcc ggagcgagct tgcggtcttc gccgagatcc
     4321 gctgcgccat cgaggagttc gccggctgcc gcgtgccgat caccggcgac ttcatctccg
     4381 gggcggcccg cagctcggcc ttcaccggct ggccgatcca ccgggtcatc gagccgggcg
     4441 atccggtgat ggccgacctc gcgccgcgca tcgacggcta ctggggcgat tcctgcgcca
     4501 ccaccagcgc cgggccggca agcaagggct tcatgaagct tttcgaggcc tccaagggcg
     4561 ccctcaccca cgcgctggaa atcatccgcc cgggcatgac cgcccaagag ctggacgcgc
     4621 agcttcgcgc ctatgtcggc cgccacggct tcacctaccc gcaccacagc ggccattcga
     4681 tcggcaccgc cgtgcacgag ttcccgcgcc tcgtgcccta cgagacggcc gtgctcgagc
     4741 cggacatggt cctcctcgtc gagcccggct gctacgaccc ggagatcggc ggcgtgcgca
     4801 gcgagtacat gatccgggtg accgacagcg ggtgcgagat cctccacccc ttcgaccacg
     4861 tgccttcggc ctagacgact ctcgtcggtc ggcgggttct cgcgcgcgtc gcgcctgccg
     4921 gccgacctat tggctctcga catgacatga gctggaaagc accggtgagc gcgcgctccc
     4981 tgcctccgga aggccccctt gtcgcctggt acggcgacga tttcaccggc tcggcggcgg
     5041 tgatggaggt gctgaccttc gccggcctgc cttccgtcct cttcctcgag ccgccgacgc
     5101 cggcgcagct cgcccggttc gaggggctgc gggggatcgg catcgccagc accgcccgcg
     5161 cccaggggcc gcgctggatg gacgccaacc tgccggcgct gttcctgacg ctgcgcgggc
     5221 tgggtgcgcc gatcaaccac tacaaggtct gctccaccct cgattcggcg cccgaggtcg
     5281 gctcaatcgg ccgggcgatc gatctcgcca tccccgtctt cggcagcgac tgggtgccga
     5341 tcctggtggc ggcacctcct ttgcgccgct accaggcctt cggccagctc ttcgcctcgg
     5401 gacccggcgg cgtctttcgc ctcgaccgcc acccggtcat ggcccgccat ccggtgacgc
     5461 ccatggacga ggccgatgtc gcccggcatc tcgcccgcca gacggatcgc cagctcggcc
     5521 tgatcggcct cgaggatctg gcaaccgccg accgggcgct cgaggcacgc cgccgccagg
     5581 ttgccgaggg cgccgccctc ctgacgctcg actgcatcga cgaggcgacc ctcggcatgt
     5641 gcggtcgcct gatctgggag gcgcgcgggc cccatcagct cgtcgtcggc tcgcagggca
     5701 tcgagtacgc gctcatccgc cactggcagg attgcggcct gcttcccagg cacgcgccgc
     5761 cggcgggcat cggcccggcc aagcggatgg cggtggtgtc gggctcggtc tcgcccgtga
     5821 cggcggcgca gatcgactgg tcgctggcca acggcttcgc cggcatcgcc ttcgacgcaa
     5881 gggtggtggt ggacaaggcc gccctggcgc gggaggagga agccgccgtc gcggctgccc
     5941 tcgatgcgat cgaccggagc ctggacccgc tggtcttcac ggcccgcggc ccggacgatc
     6001 cggccgtgcc ggcgctgcgc gaggccctgg gccggagcgg gctcgccgcc gagacggcca
     6061 acgagcggat cggcacggcg ctcgggcgga tcatcgaccg catcatccgc caggcgggcc
     6121 tgcgccgggc cgtcatctcg ggcggcgaca cctcgggcca tgccagccgc cagctcggca
     6181 tccatgcgct gaccgccctg gcgcccaccc tgcccggtgc ggccctgctc cgcgcccaca
     6241 gcgatggcgc ccatgacggg ctcgagctgg cgctcaaggg cgggcagatg ggcagccccg
     6301 actatttcgg ctggatccgc gacgggggtg gggcaagggg ctgaccggct ggttccggca
     6361 acgtcgccga cctaggattt ataccctaac tcggcgtcga atgcaagccg gcccgggtcg
     6421 gttcggcaat tttggcattc gccggcccgg ggctcgcggc gaagtcgcca agaaccacaa
     6481 aaattttctt ttttacaagg agttatggga gctcgggttc gttggccctg aaccgggttc
     6541 gttcgtcgaa aaggcgggtt cgatcgttcg aatctcgggt tcgatcgtcg attccgcggg
     6601 ttcgttcgcg gtggcgtgtt gcagaatccg cgacatttcc gggcgaatgt cgcgccggat
     6661 gcgccatcac gaatcgttat gtcgcggaat ccgcgacatt ccggcgggcc ttgtcgcgga
     6721 aaacgcgaca acccgagccc ggatgtcgcg tcggatgcac caacccgagc gattcggggc
     6781 gccggacgac aggcccgggc gtccgccggg acagagccat aagcattcgt gatgccttcc
     6841 ggcgggcctg cagcgtcagc cgaacctggc gattgccgcg gcgagctcgg ggtgggcctg
     6901 cgcctgctgc tcgagcgaga tgccggcggc cgcggcgtcc caggcctggc gggtggcggc
     6961 cacaccggcg gccgggccca tcgggtggcc gtgaatgccg ccgccaccca gatacatgag
     7021 gtccagcgtg cggccggtgc gctcgaaggt ctcgaccgcc tgcccgcccc actggccgga
     7081 gcacaccacc ggcagcggcc ggtcggcctc gctgaagatc ggcgtcatgc agtcgtggaa
     7141 ggagcgcacg aagctctcgt ccggctccca gtacttggcg cggatgccgt tgatctggag
     7201 ctggtcgata ccgagcaggc gccagatctt ctggtagggg cggaactcca tgccgagccc
     7261 ggggtggcgg gtgaggatgt cccagccatt gcggtgcgcg tgcaggcaca gggccgagcg
     7321 gcggcgcagg aaggtcatgc cgccatggcc gatcgagttg atgttgacga cggcggcgtt
     7381 gccgccggcg tcgagcacga ggtcgtggtt gcgcagcatc aggtcggggt cggccgacga
     7441 gatgccgaag gcgtacatga ccttcctgcc ggtcttctgc tcgtggtcga ggatgaccgg
     7501 catgatcgcg gcgacgcgcg cctcgagcgg cgagtagccg ggactcatca gcttctcgtc
     7561 gtccttgatg aagtcgaccc cggcctcgca gagcgtgcgg acgacctcgg cggtctcctc
     7621 gggcagcagg ccgagcgacg gcttgatgat cgaggcgatg atcggcccgc tctcgacgcc
     7681 ggtcagccgg cgcgagccgg cgatgccgaa ctgcggccct ggatggcagc gccacgcgtc
     7741 cggcaggtcg atatccatga cgcggatgcc ggtcagcccc ttcgtcgcgt agacgccgcc
     7801 gatggcgatg gtcatcagcg ccgcgacatc cgtgccgatg gcctcgagcg gataggcgat
     7861 gaccgcatcg gcgcgcttgt aggggccccg gcccccgaca tcagggatcg agggtgtggc
     7921 gagcggctcg agcggcgtca ggccgaccac ccgggcggcg catctggcgc ggacggccgc
     7981 ggtttcgccg ggcagctcgg tgaaggtccc ggtggactgg tcggaggcga tcttcgccgc
     8041 cgtcgcttcc gggtcgcccg ccgtctcgag gcgataggtg acccggatct cggccgctgg
     8101 catggcctcc acgccgagcg gcccgtcagg ccttggcgta tttcgcctcg acctcgtcga
     8161 tcgccctgac attgggcgcc gagcggccgg cctcgtcgaa ggtctgggcg aggaaggcgt
     8221 cggcgatcgc cttggccagc tccgggccga tgacgcgcgc acccatggtg atgatgtgcg
     8281 cgttgttgga gagcgccgcc cgctcggccg agtaggtgtc gtgggtcagg gcggcgcgga
     8341 tgccggccac cttgttggcc gagatgcaca caccgatgcc ggtgccgcag accaggatgc
     8401 cccggtcgta aaggccgtcc ctgacctggc tcgccacccg atcaacgaga tgggcataga
     8461 gcttctcact gtcgttctgc ggcgtgctga gctccgccac ctcgtggcga ccctgcagat
     8521 gctcggccag cacatgcgcg agctcggcac cgacgctgtc acccgcgacc gcgatcctca
     8581 tcgtcgtcct cccggttcct ctgtatctgg gcccggcgca ttcgtgcagc acgatcggcc
     8641 ggacttcaag gacggcgctg gctcgaccac cgccagcggc tcgaagcggg tcacgacgac
     8701 cggagccgcc gatcgaaggt caggacccgg taggcatacc aaagaccgag cccggcggcg
     8761 acgaggttgg aaaccgggtt cagccagttg ccgacgctct cgtattgctc gcccaggagg
     8821 tagccggcgc cgatcagcag ggccgaccag gcagcggtcc ccagggtcga gaagagcagg
     8881 aaacgccgca gcgtcatgcc cgagatgccg gcgggaacgg agatcagcgt gcgaatcgtc
     8941 gggatgaggc ggccgatcag gacggacttg cccgaatggc ggtcgaacca cgcgtcggcc
     9001 cgctcgatct cctcgggcga gagggtgatc caccggccgt gcttcgccgc ccagctcttc
     9061 agacgctcca gccccagcca ccagccgacc cagtaccagc ccagcgcccc cgcgacggaa
     9121 ccgatcgtcg ccgcgacgac cgcgccgacg aggctgatcc cgccccttcc cgccaccgat
     9181 cccgccagcg gcaggatcag ctcggagggg atcggcggaa agacattctc gaggaagacg
     9241 agcgccacga tggccagata gccaccgctc tcgacgaggc ccgagatcca gtctgccacg
     9301 gtgcgcgggc cgcttcgcga tcagcccttc gacacgtgct cgggcttccc ctttcgccgc
     9361 gtcgaggcca atcgctcgag ctcctcctcg ctcatcgatt cgaccatcga tctggaggcg
     9421 ccctgaagct cgctcttctt cgtctcgccg cgctcggcag acagcgccgc accggcggcc
     9481 ttctgctgcg ccttcgattt cgccggcatg atccgctcca tgatgttcct cgctgccagc
     9541 aacgcatcgc gcgcccggcg gttcccggca cctcacggcg accatcgacc acccctcgga
     9601 gccggtgcgg caaatccggc ccgggtgccc gagcggacgc acagccggcg ggatcaccgg
     9661 cggcgacagc gcgatcttcg tggcctgcgc catgggcacg accgggcaag gcgccggcaa
     9721 cgatcggccg cgtcggacgt tagggcagca atccatttgg gccgagaatg ctgaacagga
     9781 cccgaaatcg cgcgctgcac cgcaccggat gctgggccgg atactgctgg accctgctcc
     9841 tctgtgccgg gtgtgccggg ccgcagtcgg cgctggcgcc gggcggcgcc gaatcggagc
     9901 aggtcttggg tctgcttcgg gtgatggtcg tcggcggggc cgtgatcgcc gcgctggtgg
     9961 ggggcgttgc cgtccacgcc gcgcgcagca atcgggagaa ccaggagaag ctggggcgtg
    10021 ccctcatcat cggcggcggg gtggtcgtcc ccgctttcgc cctcgcggcg ctgctggtgt
    10081 ggtcgctgcc catgatgacg gcgttccggg cgcccggcga cggcctgcgg atcgaggtca
    10141 gcggcgagca atggtggtgg cgggtgcgct actggcccga gggcgcagaa acgccgatcg
    10201 agacggcgaa cctcatcgtg ctgccggccg gcgagcgcac cgagatcgtc ctcgacagtc
    10261 ccgacgtgat ccactcgttc tggatccccg cgctcgccgg caaggtggac atgataccgg
    10321 gccgccggac gcgcctggtc gtcgagccgc aagagccggc cgagctgcgc ggccagtgcg
    10381 cggagttctg cgggacgtcg cacgcgctga tgtccttccc ggtcgaggtg gtgccggcgg
    10441 cggcgttcgc cggctggctg gagcgcgagg cagcgcatgc ggcggcggcc gagccggggc
    10501 cggcggaagc gggtgcggag ctcttctcgg ctctcggctg tggcggctgc cacaatgttc
    10561 ggggcaccgg ggccgacggc gtgatcggcc ccgatctcac ccatttcgcc agtcggccga
    10621 gcatcggcgc cggcaccctg cccaacgagc ccgaggcgct ccggctctgg ctcgcgacga
    10681 ccggcaaggt gaagcccgat gtcatgatgc cctccttcgg catgctcggc gcgccggagc
    10741 tggatgccct gatcgcctat ctggcgggct tgcgatgagc ggcgagcacg atgcggcgga
    10801 tcgtgcccac cgcgaggccc aggccgagcg gctgctcgcc gcctggagga caccaacggg
    10861 ctggcgctac tggtcggcgg tcaacaattc cgaggtgggc gcatggtaca cggtagccgc
    10921 cttcgtcttc ttccttttcg cgggcgtgct ggcgctcctc gtgcgggtcc agctcgcggt
    10981 gccgagcaac gattttcttt cggctgacgt ctacaaccag gcgttcaccc tgcacggcac
    11041 ggtgatgatg ttcctcttcg ccgtgccgat cttcgaggcg ttctccatcc tgatcctgcc
    11101 gcagatcctg gccagccggg atctgccctt ccctcgcctc tccgcctacg ggttctggtc
    11161 gttcgtcatt ggcggcgtct tcgtctgcgg ctcgatcttc ttcggcgcag cgccggacgc
    11221 cggctggttc atgtatccgc cactgaccac ggacctcgag cagagcggca tcggcaccga
    11281 catctggctg ctcgggctca gcttcatcga ggtggcctcg atcgcggccg cgatcgagct
    11341 gatcgtcggc gtgctcaagt gccgcccacc cggcatgcgg atcaatttca tgccgctcta
    11401 tgcgtggtac gtgctggtcg tcggcggcat gatcctcttc gccttcccgc cgctcatcgc
    11461 gggtgacctg ctcttcgaga tggagcgggc gttcgactgg ccgttcttcg atcccgagcg
    11521 cggcggcgac cccctcctct ggcagcacct cttctggatc ttcggccatc ccgaggtcta
    11581 catcatcttc ctgcctgcga tcgcgctcgt ggcgatgatc gtgccgacct tcgcccagcg
    11641 cccgatcttc ggctactcgt gggtcgtgct ggcggcggtg ggcaccggct tcctcagctt
    11701 tggcctctgg gtgcatcaca tgtacgccac gggcctgccg gcgatctcga tcggcttctt
    11761 ctcggccgcg tccgaggcgg tcgtcatccc gaccggcgtg cagatcttcg tcttcatcgc
    11821 cacgctgctc accggccgcg tgatcttctc gactcccctt ctcttcattg ccggcgccct
    11881 tgccatcttc ctgatcggcg ggctgacggg cgtcatggtg gcgctggcgc cgttcgactg
    11941 gcaggcgcac gacacctatt tcgtcgtcgc gcatctgcac tacgtgctga tcggcggcat
    12001 gctgtttccg atcgtcgccg gcttctacta ctacttcccg ctcgtgaccg ggaagaagct
    12061 ctccgatcga cttggccgga cggcgttctg gctcatgttc agcggcttca acatcgcctt
    12121 tttgcccatg cacctgacgg ggctgcgcgg catgccgcgc cgggtcttca cctatcccga
    12181 ggggctgggc tgggacacgc tgaacatggt ctccacggtc ggcgccttca tcttcgccgc
    12241 cggcattctg gtcgtccttc tggacgtgat ccggccgaag cgccgccagc cctatgcgga
    12301 gcgcaatccc tggaacgccg gcacgctgga atggctctgc gagatgccgg gcaagccctg
    12361 gggtgtccgc agcattcccc tggtcaccag ccgctatccg ctgtgggagc aggcgaattt
    12421 cgtccgcgac gtcgacgagg gccgattctt cctgccggac gcggaggaag gcgagcgcga
    12481 gaccctggtc acgagcgtcc tcgatgcgga aggggtccag tgcctgcgcg tgcccggccc
    12541 caccttcctg acgatggcgg ccgccgtctt caccggcggc gtcttcatct tcgcgaccta
    12601 ccactggtat gcggcgaccg cggtcagcgg cgtcctggcg ctcgcctcga tcatcgcatg
    12661 gctctggacc ggcacggcgg tcatcccgga gaaggacgag aagcacatcg ggcacggccg
    12721 gacgctgccc ctctacatgt cggggccgcg ctcggtcggc tggtgggcca tgtgcatcac
    12781 catgatcggc gacatgacgg ccttcttcag cctcgtcttc acctatttct tcttctggac
    12841 ggtcaacccg agcttcccgc cggaagccgc tcccgggccg ggcgtcctgt ggccggccgt
    12901 tgccctcgcc atgctgatcg gcgcctgggc tctcaccacc atggcccggc gctggaacgc
    12961 gagggatcgc gtgctgtcgc tgcgggcggg tctcgctctg gccatcgtcc tggcggccgc
    13021 gagcatgggc gcgctggtct tcggcgtccg gggcaccggc ctggatccga cggccagcag
    13081 ctacggtgcg acggtctggg tcctgatcct gtggctggct gcgcatgtcg cggtgggcag
    13141 tgtgatgcag gcctactgca tcgcccggag catggcgggc aagctcaccg gcaaatggga
    13201 tatcgagatc cggaacgtca gcctgtactg gcacttcctc gcattcacgg ccgccgccac
    13261 gaccgccgtg atcgccctgt tcccgctggt ggattgagcg aatggcggcg aacaggcaag
    13321 gcagtcgcgg cctctggacc ctgttgacgg ggcctgtcgt ctgggccgtc cactttctcg
    13381 tctgctacat cggcaccgcc atcttctgcg ccagggcagg cgatccggag gccgatctgg
    13441 cgcccgtccg gctcggcatc ggcgccatca cgctggtcgc cctcgccgtc atcgccttcg
    13501 tcggcatcga ggccgaccgg cggagcgggc cggggctggt gttcgagtgc ccgcacgacg
    13561 aggacaccgt ccgcgagcgc cgccagttcc tcggctatgc gacgctcctg ctcgccgggg
    13621 tcagcttcat cgcgacgctc ctcgtcgcgc tgccagccct cgtcatcccg agctgccgat
    13681 gagccggctc ggcgccgcgg ccgtgggcgg actgctgctc gtggcggcat gggccgggcc
    13741 gctcgccgcc cacgcggcga gcagcttcac ggcccacatg gccattcatg tcaccgtgat
    13801 cgcgatcgcg gcaccgttgc tcgccttcgc gatgggcccg cgcttcgccc tggcccggcg
    13861 ctgcccgctg ctcttcgcgc cgctcgtggc ctcgctgctc gacatcgtcg ccatctgggg
    13921 ctggcatgca cccgccccgc acgtcctcgc acggacctcg gcgctcggct tcgcgctcga
    13981 gcagctctcg ttcctggcgg tggcgctgct ggtctggacc agcgcgctcg gcgccgggca
    14041 gtcggagagc cgggcgggcc aggccgcggg tgtcggcgcg ctgcttttca cctcgatgca
    14101 catgacgctg ctgggcgccc tcttcgccct ggcgccccgg gagctcttcc cccgggagat
    14161 ctgcggctcg gcctttgccg gcctcacggc gctccaggac cagcaactgg ggggcacgct
    14221 gatgctcgcg ctcggcggtg ccagctatct ggccggcgct ctctatctgc tcgcgcgcct
    14281 cctctccgac ggcgcgctcg gggctcgtga tgcgcgtcac ggctagaggg ctggctctgg
    14341 ggctggccgg cctgctcgcc gcgggggccc ttttcgcctg gtcggggctc ctcaacgtat
    14401 ccgccagctc cggccactgg gcgatcacca gctggttcct gcacgcggtc atgcgccagt
    14461 cggtcgagac ccatgcgggc gggatcgagg cgccggacct cgatgacccg gccatggtcc
    14521 tgcgcggcgc cggtcacttc gccacgggct gcgcctattg ccatggcgca ccgggcaaac
    14581 cgccgagccc cgctgtccgg aacatgacac cggcgccccc gcgcctcggc gggacgttgc
    14641 gcgaatggga gcccgccgag ctcttctgga tcgtccggca cgggatcaag ttcaccggca
    14701 tgcccgcctg gcccgccccg gagcgggcgg acgaggtctg gtcggtcgtg gccttcctgc
    14761 gggcgttgcc cgggatggcg ccggagacct atcggcggct cgcgcacggc cccgcaagcg
    14821 cggaagggcg agcggatgcg ggcgccgcgc tcacgagttg cgcccgctgc catggcttcg
    14881 acggcagcgg tcggggggcg ggcgcgtttc cggtcctgtc cgggcagaac gaagcctacc
    14941 ttctcgccag cctcagggcc ttcgccagcg gctcgcgcgc cagcgggatc atggagacgg
    15001 cggtgaatgc tctcgatgac gaggcgctca gcgatctcgc ccggcattac gcccgccagc
    15061 cccggccgga acggccggcc gcgccgccgc ctgccgccgc cctggcagcg gagggcgagc
    15121 gcatcttcaa cgaagggctg ccacgccagc gggtcccggc ctgcgccagt tgccacggtc
    15181 ggcgaggcga cgcgcctcgc tatccctcct acccccggct cgccggccag ccgcgccact
    15241 acctcgagac ccagctcgag ctgttcgccg ccggcagccg gggcggcacg tcgttctccc
    15301 atgtcatgac gatggtggcc gggcgcctcg agcccgccca gatcgaggcc gtcgccgcct
    15361 ggctggcgag cctgccgccg caatgacgcc gcccggcgga acgtccggcc gtgcccgcag
    15421 gttgtcacgt tcggatcaaa ggagacgtcg cttggcaccc cgcgccatct ggaaaggaca
    15481 gctccagatc agcgagctgg tctgccccgt ctcccttcat gcggctgcgt cgaccgccgg
    15541 aagggtgtcc ttccacctgg tcaatcgcgc gaccggcaat cgggtccgcc gcgaatacgt
    15601 ggacgcggaa accggcgagc aggtcgagcg cgagcagcag gtcaagggct acgcaaccga
    15661 cgacaggcgc tacatcccga tcgagccgga ggaatatgcg gcggcgctgc cggagaacca
    15721 caagacgctg caggtcgagg cgttcctcga ttgcgccgat atcgacaccg tctatctcga
    15781 tcgcccgtat ttcctcctgc cggccgagcc gacggccgag gaggccttca ccgtcatcag
    15841 ggagggcctg cgcgccaggc ggaaggccgc ggtggcacgg gcgatgctct ttcgccgggt
    15901 acggtcgctg ctcatccggg cgcagggtcg agggctgatc gcgagcaccc tgaggttcga
    15961 ttacgaggtc cggcccgcgg aggaggcttt cgccggcatc catgagcagg cgatccaggg
    16021 cgagatgctc gacctggccc ggcacatcat cgcgagcaag cgtggcgagt tcgaccctgc
    16081 cgcgtatgac gatcgctacg atgcggccct ggccgggctg gtgaaggcga aggcggcggg
    16141 ccggaagatc cgcccgcgcg ggcgacgccc ggaaggccgg gtcatcgacc tcatggatgc
    16201 cctgcgcaag agtgccgagg gcgccgggcc cgccagccgc cgagaggctg cctcgcgcaa
    16261 ccgaaaggca ggttgaccat ggcgctcgac acctatcgtg aacgacggga cttcaggaaa
    16321 tcgcccgagc ccagggggcg acccggccgg aggaggaccg cccgcaaggg aagggcgttc
    16381 gtcgtgcaga tgcatgcggc gcgccggctc cactacgatc tgcggctgga aatggatggc
    16441 gtgctcaaga gctgggccgt gacccgcggc ccgagcctgg tgccgggaga gaagcggctc
    16501 gccgtgcatg tcgaggacca cccgctcgaa tacggggact tcgaagggac cattcccgcg
    16561 ggctcgtacg gcgccgggac ggtgatcgtc tgggacagcg gctcctggca gccgatcggc
    16621 gatgccgcca agggctacgc caaggggcat ctcgacttcg agctcgccgg cgagaagctc
    16681 gagggtcgct ggcacctcgt gcgcatggcg gcgaagccgc gcgagaagcg cgagaactgg
    16741 ctcctgatca agggggacga cgagcacgcc cggggcgcgg atgaccccga catcctcgag
    16801 gagcgccccg agtcgacgaa gaccgggcgt cggctggagg aggtggccga atcggccccg
    16861 aagaaggaga aacgggcgcg acccgccggc aagcgctcga gccgcccgct cggggacgcc
    16921 cgccaggccc ccttcccggg cttcgtggca ccggctctcg ccacgcttcg tcccgaggcg
    16981 ccgcgcggcg agcgctgggt gcacgagatc aagctcgatg gctatcgcct ccaggcccac
    17041 gtccagggcg gcgaggcgag gctgctggcc cggagcggcg ccgactggac cgagcggttc
    17101 ggcgaggcga tcgccgccgc cctggccgcg ttgccggcac gcgacgcggt gatcgacggc
    17161 gaggtcgtcg tcgaggcccg caacgggtcg tcggatttcg ccgcgctgca gcaggacctg
    17221 agcgaggaac ggaccgacag gttcgtctac tacgccttcg atctcctcta cctcgacggc
    17281 tacgacctgc ggccgggcgc gctcgaggat cgcaaggcgc ggctcgagca cctgctcgat
    17341 ggctcgccgc cggcgctgcg ctacaacgat catttcgacg aggagggcga gctggtcctg
    17401 cgccatgcct gccgtctgag cctcgaaggc atcgtctcga aggagcggca ggcgcggtat
    17461 gcgtccgggc gcagcacgag ctggatcaag tcgaaatgcc gggagcggca ggagctcgtg
    17521 gtggcgggct acgtgccctc ctcgacgctg ccgaagtccg tcgggtcgct cgtcctcgcc
    17581 tgtcacgagg gtgggaagct cgttcccgcg ggccgggtcg ggaccggctt cagccgcagg
    17641 atggcagccg agctctatcg ccgcctcgcg cccctggagc gggcgaagcc acccttctcg
    17701 gggtcgccgt ccgccatcga cgcggcggac cgccggcagg tccgccatgt cgagcccgag
    17761 ctggtggtcg aggtcgagtt ccagagctgg acggcgggtg ggctgcttcg tcatgcctcg
    17821 tttcgtggca tccgcgagga caaggcgccc ggcgagatcg tgcgcgaggg cgagccgaag
    17881 agaggtgccg gcgatcgggc gccgcgcgcc gtcaggctca cccatcccga ccggctctac
    17941 tggcccgagg ccggcgtcac caaggagggg ctggccgatt actatgccga ggtctggccc
    18001 tggatggcgg cgttcgtcgt cggccggccg ctggccctcc tgcgttgccc gcagggcgcc
    18061 acccgggcct gcttcttcca gaagcatgcg tggaaggggc agagccggga aatattgaag
    18121 gccctggacc cgctcgacga atcgggcgat gccagcatgg tggcgatcga cggtctcgac
    18181 ggtctgatcg ggctggtcca gggcggtgcg ctggagctgc atggctggca gtcgtcgctc
    18241 gatgcgctcg agcagccgga ccagatcgtg atggatctcg atcccggcga cgacgtggcc
    18301 tgggacgcgg tcatcgcggc ggccggggaa gttcgcgatc gtttggccga ggcgggcctg
    18361 cagagcttcg tcaagacgtc gggcggcaag gggctgcacg tcacggcccc gctgcggccg
    18421 aaagccggct gggacgaggt gaaggcgttc gccaggtcga tcgccgacgc catggcggcg
    18481 gaccatcccg accggtacgt cgcgacggtc gccaaggcga agcggcgcgg ccggatcctg
    18541 atcgactacc tgcgcaacgg gcgcggtgcg acggctgtcg tgccctgctc gaccagggcg
    18601 cgcgcggggg cgccggtcgc catgccgctg ggctgggacg agctgggccc ggcaatcggg
    18661 ccggcccatt tcaccgtgct cgatgccgcc gggcggctgc gaaacctcgc tcgcgacccc
    18721 tggggcgagt tccgcaaggc cgcggcgacg ctgccgccgg caaggtcgcc aaggcgtcgc
    18781 cgctgagcct cagcggcgca tttttcccac ctggccctgc aattcctcga ccgacgggcc
    18841 tcgccatcgg cgggtcgggg cttcacgcga tgcactggcg gcccgggcac ttgcgttcac
    18901 catcctcacc gcggcgcgct ccggcgaggt gcgcggcctg cgctggggcg agatcgacac
    18961 ggcgcaggcg gtctggacag tgccgggcga acggatgaag gccgggctgg agcatcgcgt
    19021 gccactggtg cccgaggccg tcgcctgtct gccgccgcgc gatgacgatc cggcggcgct
    19081 ggtctttccg ggtgccatcg acgccgaacg gccgctctcc gacatgacgc tgctcgccgt
    19141 tctcaagcgc atggggcgca gcgacctcac ggtgcacggg tttcgctcga cgtttcgcga
    19201 ctgggccggc gagagcacga cgcatgcccg cgaggtgatc gaggcagccc tcgcccaccg
    19261 cctcaaggat aaggccgagg ccgcctatgc tcggggcgat ctcttcgcca agcggcgcct
    19321 cctgatgcag gattgggccc acttcgcgct gtccgcggcc gcctgatcgc cggaactcaa
    19381 tcgaggattt cccgaactcc ctacctgagg agcctgctgt tgatcgatcg ctcatccccc
    19441 gccgatgcgc accagccgtc gtcgaacccg ctgacctgca ccggagccct gaagatcgtc
    19501 cgcctggagc ggccggaaga tctgcatgaa caacttgcca aggccatcca ggccgagttg
    19561 accgaagcgc aggtcgacgc cctcttgaaa gtgctggagg actatgtcaa tcgcgggctc
    19621 cgcgccgccc gcgtcccgga ggtcggaggt ctccgacggc ggctgaacaa gctcgcccgc
    19681 ctgaccgacc agctggcgcg gctgctgatc gagatcgaac agaagggcga gggcgactgg
    19741 tacgccctga cgcgggtcat cggccaccag gaattgtcga cttactgcgt cggcacgggg
    19801 ctgatcaagc aggtggcgta caagaccgag agcgatctgg tcgccgtgga ccggcgcgcc
    19861 tgggccagct tcgagggcct cgtccatcag cttgccgaca tcttcgagca gaccggacgg
    19921 cgggccaccg cccagtgcac
//
LOCUS       DAOTFI010000070        19813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3296_length_19813_cov_6.982437, whole genome shotgun sequence.
ACCESSION   DAOTFI010000070 DAOTFI010000000
VERSION     DAOTFI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19813
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_19813_cov_6.982437"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="P5558_08985"
     CDS             <1..254
                     /locus_tag="P5558_08985"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24482.1"
                     /translation="LVVSVMGGHGCLFGRGNQQIAPSVIARVGRENIQVVASLAKLAA
                     LGTAGLFVDTGDPAVDAMLEGFIKVRIGPATTVMMRVRG"
     gene            complement(292..747)
                     /locus_tag="P5558_08990"
     CDS             complement(292..747)
                     /locus_tag="P5558_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY24483.1"
                     /translation="MPNNRSAYVFDTNVLSEVMKERPDAAVVDWLRHCPAEAMLTTSI
                     SQAEILFGVRRLPEGARRARLLAAAVALFTTTLAGRILAFDEQAASIYADIRIAGERA
                     GRPVAEQDGMIAAITRAHNVRVLTRDERGFADCGVAVVNPWKSTETLGG"
     gene            complement(747..992)
                     /locus_tag="P5558_08995"
     CDS             complement(747..992)
                     /locus_tag="P5558_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HRY24484.1"
                     /translation="MSNITIRKLDPTVKERLRVRAAHHGHSMEEEVRRILKQAVSDSA
                     DEPRSAYDAIRRHFAGLGGVELELPARRPGRAPPAFD"
     gene            complement(1075..1596)
                     /locus_tag="P5558_09000"
     CDS             complement(1075..1596)
                     /locus_tag="P5558_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY24485.1"
                     /translation="MTNEELRRLNAKRRIHQIAFVTRDLERSMQAWIDNLGVGPWMVL
                     TFTERSLTYLKVEDRTVSEPFEFLLALAWMGDTQIELIQPVRGATIYEDHLRRKGEGL
                     HHIKEKIADADMDRVVADYREKGIGVMQTGLFEGDVHYYLNTEPRLDFIHELGNCLDL
                     KLTPEQYRMYPSE"
     gene            1751..3127
                     /gene="gcvPA"
                     /locus_tag="P5558_09005"
     CDS             1751..3127
                     /gene="gcvPA"
                     /locus_tag="P5558_09005"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRY24486.1"
                     /translation="MANSVAGIKQAMLDAIGAGSIEELFAQIPASHRMKGKLGLPPGM
                     KSEAELRRHLVGLLARNQTCEANLSFLGAGCWQHHVPAACEEVVRRRELLTSVFGSPA
                     SDHGRNQAWFEFTSQLGELVDCDLVGLPVYSWGAAAGHAIRMAERLTGRRRVLVPRNI
                     DPERLGVMRTMAEPVEMPHHITISELDWDPATGGIDLAALDAALSDEIAAVYFDVPAY
                     LGGIEPNAAAIARKTKARGAMVVVGVDPISLGIVAPPGAYGADIVVGTLQTLGIPMNC
                     GGGVGGFIASRDEERIAREYPTLFASIAETVQPGERGFALSLFHQSSYGSREKGRDWT
                     GNSVYLWGIANAVYMGLMGPAGFREVGELIIARAHYAAKRLARVPGAAIRWRSGFFKE
                     FVVDFTGTGKTVHEINAALRAHGIFGGKDLSADFPELGQAALYCVTEIHNQADIDRLA
                     ETLEEVVR"
     gene            3124..4692
                     /gene="gcvPB"
                     /locus_tag="P5558_09010"
     CDS             3124..4692
                     /gene="gcvPB"
                     /locus_tag="P5558_09010"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRY24487.1"
                     /translation="MSGNDTKLRRYQAPVWDEPVIMEMSSPGRRGQVFTAPEPAVADA
                     VGDIAALLPAGMARTAPPALPEMSEHDVLRHFLHLSQETLGMMGISLFGTCTMKYNPA
                     LGEALAWRPEIAALHPLQDEDTLQGVLAIVHGLDRQLRELSGMDQFVFQAGGGAHAGY
                     TNCCVTRAYHASRGELAQRNQIITSIQSHPCNPATAAAAGFEVITLMIEENGYPSLDA
                     LKAAVSDRTAALMIGNPDDMGIYNPEIVEWIEIVHAAGGLCFWDHANFNGVMGRVRAA
                     ELGFDACMFMLHKTFGAPKGGNGPSVGAYGCTKELARFLPRPIVVEEAGRFRLDHDRP
                     DSVGKVREFWGNVPQLMKAYAWTCAMGSDGIAEAADLSVLANNYMDAKLGRIRGLSKS
                     HPQLTAPRMEMTRWSLGPLKEETGVGTLDVQNRMVDFGVDAFWLSHEPWLVPEPFTPE
                     AGELWSKEDLDYWVAVIERISEEAYSTPELVKSAPHNQTIHQVKYDGLEDPERWAMTW
                     RAYVKKRERRGEAA"
     gene            4908..5561
                     /locus_tag="P5558_09015"
     CDS             4908..5561
                     /locus_tag="P5558_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24488.1"
                     /translation="MPLLEACVLGQGVFMPGGEAEVVFVESRPVLAEIGGRPVLSLKE
                     FLAADGRDRLFNVAIAESRIREELALACLDAGGLAQTLVAGDAEIFPSVTIGDGAIIC
                     GRTMITDNVTIGRFFHCNIYSYVAHDCVIGDFVTFGPRVSCNGNVRIDDHAYVGAGAV
                     IRQGAPGRPLVIGKGAVVGMGAVVVRDVAPCTTVVGNPARPLDPAILDGPEVLSATG"
     gene            complement(5545..5988)
                     /locus_tag="P5558_09020"
     CDS             complement(5545..5988)
                     /locus_tag="P5558_09020"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY24489.1"
                     /translation="MTTLWLGLVLLGGILLGIQPTLNAGLARHLGHPLSASLVSFGTA
                     TVLLLAVAMALRPPVPALADLRLVPPWLWLGGAMGAAFVTIALMATPRLGAAPVVALV
                     ICGQLAAALVIDSFGLMGLTPRPFDWTKLLGALCLVAGAILIRWR"
     gene            6057..7283
                     /locus_tag="P5558_09025"
     CDS             6057..7283
                     /locus_tag="P5558_09025"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY24490.1"
                     /translation="MDGNEETGSAGRAAAGLTRAELAKLASCPAAELDWLVGLGLIRP
                     DPGGRFAHGDISRVRLVMALLGSGLGRAQLEAAARAGQISVDFAGDMIADAPALTGLT
                     HGEALAAIGLDADEASRIGRALGLPAAAPGDPIREDDRELLALVARARQEGIGEAAIL
                     RMLRVFALGIRQIVEAQRDLFRENVEEPLLASGMQRRELLAKTAPLRPRLQRLGYRAV
                     FLILRRLLEQAVFDNAMARLDESLAEQGIVRPAAADRTIVFIDLSGFTALTEAEGDAR
                     AAMLGSRFLELAQEAALHHAGRLVKLLGDGAMLHFRRPEDAVAAALAIIAGAPGHGLP
                     PARAGIATGPVIGRDGDCFGRTVNIAARLADAVPAGGIWLDAATAAHAGRPLVPAGRR
                     SLKGLVEPVEVFGLDP"
     gene            complement(7351..7941)
                     /locus_tag="P5558_09030"
     CDS             complement(7351..7941)
                     /locus_tag="P5558_09030"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HRY24491.1"
                     /translation="MDPYPLLKFGHVLGFILLGGGLLAVFVSELRAYRTSDLTVFTEA
                     SHYTALFYDSLAVPGAVLVGVSGFFLVYMLGLGFFEEPWLVGMWGLFLFELIEGNTVT
                     RIQFTRTRRRSRAALAEGRPLTDDLRDEARSTAGQVAHFLDLPLFTVIVYCGTVRPGA
                     WSEVGVAIMLALLAATALTFAVPRLARRATGAHQRE"
     gene            complement(7951..8433)
                     /locus_tag="P5558_09035"
     CDS             complement(7951..8433)
                     /locus_tag="P5558_09035"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HRY24492.1"
                     /translation="MLKLVKALHLLGAMLFFGSILAHVTTGLIPGAEDDPQTALVIRQ
                     AIAIATSYLTMPGLILLLVTGTSMIVKARLPIFRLRWLTLHALFGLLIALNAALVLYP
                     TGREALEVATQVAAGLLPIDQLHALGAREATFGPVNVLLCLATVLVAVIRPRLGKARA
                     "
     gene            complement(8438..8896)
                     /locus_tag="P5558_09040"
     CDS             complement(8438..8896)
                     /locus_tag="P5558_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY24493.1"
                     /translation="MSTHTPEAAVLTALILEAFRLNGRLLADGDRLTGDLGLSSARWQ
                     VMGAIEAVPLPVAQIARDMGLTRQAVQRLANALADEGLVTFELNPNHRRAKLVHLTDR
                     GRLALNEVNRRQVEWSNRLAAGLAIETIEAALALMHTLRQRLEADHTEEA"
     gene            complement(8998..9711)
                     /locus_tag="P5558_09045"
     CDS             complement(8998..9711)
                     /locus_tag="P5558_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24494.1"
                     /translation="MPALLPADGRVVMISGASRGIGAAIADRLLTDGYSLSLGMRTPT
                     PPTDPERILTHRFDALDPPTAGAWVEATLQRFGRLDALVNNAGIFRTVTFEEGSEDDL
                     DELWAVNVKGPFRLTRAALPHLRAAANGRVVNICSTDGKRVRDRSASLGYVMSKHALV
                     AMTHATRFAGWDDQLRATALCPGAVETELVQNVPGAVPGPGRIEPETIAETVSLLLSL
                     PDNASVAELVVNTRLESML"
     gene            complement(9713..10495)
                     /locus_tag="P5558_09050"
     CDS             complement(9713..10495)
                     /locus_tag="P5558_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24495.1"
                     /translation="MDLGIKGRKAIVSAASKGLGRACALSLAEAGCDLWLMARTKEAL
                     EEAAHAIAEGTGVQAQAIAGDFTAEDDRAKLLAACPAPDILVNNPGVRQTPTPFADIT
                     KDEWRHWFEAHCFSSLDLIQRCAPGMAERGFGRIVNMSVSFIKFPQVNFAHSHAARLA
                     LSGAVASMTRDLIGKNVTINTVCPGLFDTDALHTNLHGHAKRGNTTYEAIVQDRLEGC
                     PAGRFADPKECGDLVAFLCSAQAGFMTGQNIVNDGGVYQGLF"
     gene            complement(10499..11416)
                     /locus_tag="P5558_09055"
     CDS             complement(10499..11416)
                     /locus_tag="P5558_09055"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY24496.1"
                     /translation="MDRNSVAWSGAMPAVITPFDAKGAIDTKAFTANLERQFDHGATG
                     AVVAGCTGEFWTLTLAERATLFRLAVEVAQGRTILAGTGAILAADAIASARAAADAGC
                     DGVLVLPPYFVKLTDDEIFGHIETVAAASPLPVCLYNIPGNAVNAIPPALAARLADVD
                     KVVAIKESSGDWNNFYGTHLAVADRLRVFCGPSSVFGVPAVELGADGVIDCFPNVWLP
                     GGIALFEAAKDPARRAEAAALQATGRRLTDLFTTGGRTLYPATKAAMEMLGWQAGEPR
                     APLRRLEGEPLAGLERGLVELGLLNKKAA"
     gene            complement(11421..12482)
                     /locus_tag="P5558_09060"
     CDS             complement(11421..12482)
                     /locus_tag="P5558_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24497.1"
                     /translation="MSEVVFEGVRKAFGAAVAVESLDLRIESGEFVSLLGPSGCGKTT
                     SLRMLAGLEVPTSGTIRIGDRVVNDIAPGKRDIAMVFQSYALYPHMTVAQNIEYPLKK
                     RRIPKPERGPKVAEVAKLLHLEDYLARKPRQLSGGQQQRVALGRALVRDPAVFLLDEP
                     LSNLDAKLRAHMRAELIELHQRIGRTMVYVTHDQLEAMTMSDRIAVLANGRLQQLDTP
                     AMIYRQPANRFVAGFIGTPSMNLVEGRLEHGAFVAGEWRLSVEGLALDRPTGAVTLGI
                     RPEDILIDPAGQPARVKLVEPTGHEAIVQLEIAGHQLTGRVGAEIALRAGEPLGLSFR
                     TANLHLFAGEDGRRLNKES"
     gene            complement(12487..13323)
                     /locus_tag="P5558_09065"
     CDS             complement(12487..13323)
                     /locus_tag="P5558_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY24498.1"
                     /translation="MRRTSLPGRIAFNLLAWTLVLLVAFPLIWMILTSVKPQTELFRI
                     PPSFWPTEITFEHYRRLLVETPFLTYMRNSIFLALGTTLLVITLATLGAHSLVRFRYP
                     GRERLAQLVLFTYLLPSVVLIIPLYLLMVKIGISNTLWSLILAYTTFALPYALWLLRS
                     FMAGIPDDLEAAALVDGASRMEAFIDVILPQALPGIISTALFTAILAWNEYLYALVLV
                     NTDSARPLTTGVMTMLISAFNIEWSLLMAASVMMSLPLIVAFAFLQKYLTRGFGAGGV
                     KG"
     gene            complement(13323..14234)
                     /locus_tag="P5558_09070"
     CDS             complement(13323..14234)
                     /locus_tag="P5558_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY24499.1"
                     /translation="MASATPARRLSLDTQYSILGLLLIAPTVLVFTAVIVYPLLSAIY
                     LSLFSIYTPTLQGEWVGLANYQRLLAKGEFWISLRNTLVWTAGTLTLQIVLGVAMALV
                     LNQSLLFRPLARSLVLFPYFVSTVVAVLVWRWVFNDLYGILNHTLMRVGVLDMPLDWL
                     GSMPNAMLSIILVGAWKYFPFVVIAVLARLQSIPDALYEAAEIDGAGPIARFLDITLP
                     QLRDVLVVIVMLRAIWDFKEFDLIYLLTGGGPVISTQTLSLMVYKEAFALNQMGGASA
                     VAVLMMLVMLVFMILYLRRTARGEGNS"
     gene            complement(14246..15085)
                     /locus_tag="P5558_09075"
     CDS             complement(14246..15085)
                     /locus_tag="P5558_09075"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY24500.1"
                     /translation="MKIIDFRMRPPLGSFLQSRIYAAADNRNRYTRSLGFEPPASAER
                     QSIDLLLEEMGAAGIARGVVVGRNSPTLGVIPNDDVVAMCARYPDRFVPVGSIDPTNR
                     KAAMQAIDEAVALGVKAINIEPGVAAPPLHSDDRRLYPIYAHCEDQGIVLIVMSGGAA
                     GPDISYTAPEHIDRVLGDFPTLKIVASHGNWPWVQEIIHVAFRRANLYLSPDMYLHGL
                     PGTADYVTAINGFLADRFLFGTAYPFCPLDDYLARFLALPIRESVLERVLHDNAAALL
                     GLD"
     gene            complement(15135..16463)
                     /locus_tag="P5558_09080"
     CDS             complement(15135..16463)
                     /locus_tag="P5558_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24501.1"
                     /translation="MTIVEASTTRRRMLAGSSALGLAAALPFGRARAQDTTVRWWAAQ
                     ASPEQVAAYELQIGAFEAAHPGVKVVFEKTSDEGYAPQLAAAFASGELPDIVTHLPSF
                     AVSNYWRNGLLEPFNEVIEAIGPEKFYPGQNRIYEIDKGQYAGTGIGNAAANMLWVRT
                     DLMEQAGIADIPVTWDELRAACQAMQGGGVYGAPLPFARNGMTSLTIIGFIHLAGGQV
                     FSPDLEVALESDETLNALEFYKSMRELCPPGATGYSWGESLGAFVSGASATAIYAGRV
                     LQNVVTQNPAIADAITCATYPRVSTDVEPWTFNGFPSVFIPKDAPNKAEAKLFAAFLF
                     EPEGYIRQLHGAPGHLLPVLRTIGQDPRYLDNPIIQRYPEEVELMSEAAAAGYNLGWE
                     SPAHQPNAKAGEVVNSLVLAEMVQRVCVNGEDARSVVGETAQRIDEIMKG"
     gene            complement(16770..18098)
                     /locus_tag="P5558_09085"
     CDS             complement(16770..18098)
                     /locus_tag="P5558_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24502.1"
                     /translation="MTIVGTPFSRRRMLAGTSAMGLAAALPLRPAHAQDVTIRWWSPQ
                     ASPEQLAAYEFQIAAFEALHPGVKVVFEKTSDEGYAPQLAAAFASGEVPNVVTHLPSF
                     AVSNYWVNGLLEPFNEVIEAIGTEKFYPDANRIYEVDPGQYAGTGIGNSAANMLWVRK
                     DLMEKAGIADIPVTWDELRAACQAMQGGGIYGAPLPFGANSMTSLTIIGFIHLGGGQI
                     FTPDLEVALDSDETRSTLEFYKSMRELCPPGATGYSWGESLTAFVSGATATGIYTGRV
                     LQNVTTQNPGIADAITCATYPRISTDVPPWTFNDFPSVFIPKAAPNMAESKAFAAFLF
                     EPEGYIKQLLAAPGHVLPVLRTIAPMPQYQENPIIQKYSAEVELMAEAAAAGNNLGWE
                     SADHKPNPKAGEIVNSNVLAEMVQRVCLNDEDIATVVGETASRIDDIMKA"
     gene            18180..18989
                     /locus_tag="P5558_09090"
     CDS             18180..18989
                     /locus_tag="P5558_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRY24503.1"
                     /translation="MRNGKDVEGSARGMAENGDDRDFVNSLARGLEVIRSFTRARPRM
                     TLSEVAAETDLPRAAVRRFLLTLVREGYAATDGKLFQLRPKVLELGYSVIASLSIVEV
                     AQPVMADVSARLNESCSVAVLDGDEIVYVARSSAKRVVNIAATIGGRLPAYVTSMGRV
                     LLAAQDDAALDAYLARISPEKLNARTVTSRVKLRDAILEARLQGWSLVDQELEPGLCS
                     IAVPLRDRAGRTVAALAIGAPSGRVDAATMRERFLPVLLEAAQQMAMAMPD"
     gene            complement(18997..>19813)
                     /locus_tag="P5558_09095"
     CDS             complement(18997..>19813)
                     /locus_tag="P5558_09095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY24504.1"
                     /translation="RAKAARERALGLEVEILDRRALDRIAPYLGPAVVGAERCASEGK
                     VDPLAANTAIAAATRAAGVALQRGTRVLGITPDGAGYALRTTAGMVRAGRLVIAAGAG
                     TGALLAPLGVAVPTAAEPLHMNVTEPAAPLIGHLVQHAERMITMKQLATGQLVIGGGW
                     PARAGKGGRPPEVLRTSLAGNLGLAVHIVPEVARARLLRSWAGINTTTDGRSVLGPVP
                     GRPGLYVALPGDAGYTLGPLVARLVADHMLGRRPELDLEAYTADRFRTKAPAF"
BASE COUNT         3173 a         6198 c         7391 g         3051 t
ORIGIN      
        1 cgctcgtcgt ctcggtgatg ggcggccacg gctgcctctt cggccgcggc aaccagcaga
       61 tcgccccgtc agtcatcgcc cgggtcgggc gggagaatat ccaggtggtg gcatcgctgg
      121 cgaagctcgc cgcgctgggc acggccgggc tcttcgtcga cacgggcgac ccggcggtcg
      181 acgcgatgct ggaaggcttc atcaaggtgc ggatcggacc ggcgacgacg gtgatgatgc
      241 gggtgcgggg gtgaggagac gcaggttccg cgccaacatc agcgatctga atcagccgcc
      301 cagcgtctcg gtcgacttcc aggggttcac gaccgctacg ccacaatctg caaatccccg
      361 ttcgtcccgc gtcagcactc tcacattgtg ggcgcgggtg atggcggcga tcatgccgtc
      421 ctgctcggcg acgggacggc cagcgcgttc gccagcgatc cggatatccg catagatcga
      481 tgccgcctgc tcatcgaacg ccaggatgcg gccggcaaga gtcgtagtga agagcgcgac
      541 cgctgcagca agaagccgcg cacgccgcgc accttcgggc agtcggcgca cgccaaagag
      601 gatttccgcc tgcgagatcg atgtggtgag catggcctca gccgggcaat gcctgagcca
      661 atcgacgacg gccgcgtcgg gcctctcctt catgacctcg gagagaacgt tcgtgtcgaa
      721 cacgtaggcc gatctattgt tgggcatcag tcgaacgccg gcggcgcgcg cccgggccgg
      781 cgtgcaggca gctcgagctc gacgccgccg agccctgcga aatggcgtcg gatcgcgtcg
      841 taggcgctgc gcggctcgtc tgcggaatcc gacactgcct gtttgaggat gcgccgaacc
      901 tcctcctcca tggaatggcc atgatgcgcc gcgcgcacgc gcagccgctc cttcacggtc
      961 gggtcgagct tgcggatggt gatgttggac atggtcgttt cagacaagaa agggctatgc
     1021 acgcaatgac tctatcattt gcatgcaacg ccagcaagcc cgtgtccagc ccctctactc
     1081 agacggatac atcctgtact gctccggcgt cagcttcaaa tccaggcaat tgccaagctc
     1141 gtggatgaag tcgaggcggg gctcggtgtt caggtagtag tggacgtcgc cctcgaagag
     1201 cccggtctgc atgacgccga tgcctttttc gcggtagtcg gcgacgacgc ggtccatgtc
     1261 ggcatcggcg atcttctcct tgatatggtg gaggccctcg cccttgcggc ggagatggtc
     1321 ctcgtagatc gtcgcgccgc ggacgggctg gatcagctcg atctgcgtgt cgcccatcca
     1381 ggcgagggcc aggaggaact cgaaaggctc gctgacggtg cggtcctcga ccttcagata
     1441 ggtgagcgac cgctcggtga aggtgaggac catccagggg ccgacgccga gattgtcgat
     1501 ccaggcctgc atgctccgct cgagatcgcg ggtgacgaag gcgatctggt ggatccggcg
     1561 cttcgcgttg agccggcgca actcctcatt ggtcaagtgg catcctccct ggctggcgcc
     1621 ttcgcccttg agcgggcgcc ggtgatcgtc gatagtcgca caaacgttcg cccagcgccc
     1681 gggtcacgcc aactgccggg cttacggagg ttgcgtgcca gagaaccagg tttccacagc
     1741 ccatccctac atggcgaatt ccgtcgccgg cataaagcag gcgatgttgg atgccatcgg
     1801 cgccggcagc atcgaggagc tgttcgccca gatcccggcc tcacaccgga tgaagggcaa
     1861 gctgggcctg ccaccgggca tgaagtccga ggccgagctc aggcggcatc tggtcgggct
     1921 gctggcgagg aaccagacct gcgaggccaa cctgagcttt ctcggtgccg gctgctggca
     1981 gcatcacgtg ccggcggcgt gcgaggaggt ggtgcgccgg cgcgagctcc tgacctcggt
     2041 cttcggctcg ccggcctcgg atcatggccg caaccaggcg tggttcgagt tcacgagcca
     2101 gctcggcgag ctggtcgact gcgacctcgt tggcctgccg gtctatagct ggggggccgc
     2161 agcgggccac gcgatccgca tggcggagcg gctgaccggg cggcgccggg tgctggtgcc
     2221 gcggaacatc gacccggagc gcctcggcgt catgcgcacc atggccgagc cggtcgagat
     2281 gccccatcat atcaccatca gcgagctcga ctgggacccg gcgacgggcg gcatcgacct
     2341 ggcggctctg gatgcggccc tctcggacga gatcgccgct gtctatttcg acgtgccggc
     2401 ctatctcggc ggcatcgagc ccaacgcggc ggcgatcgcg aggaagacca aggccagggg
     2461 ggccatggtc gtcgtcggcg tcgatccgat ctccttgggc atcgtcgccc cgcccggcgc
     2521 ctatggggcc gacatcgtcg tcggcacgct gcagacgctg ggcattccga tgaactgcgg
     2581 cggcggggtc ggcggcttca tcgccagccg cgacgaggag cggatcgccc gggagtatcc
     2641 cactctcttc gccagcatcg ccgagaccgt gcagccgggc gagcgcggct tcgccctctc
     2701 gctcttccac cagagctcct acggcagccg cgagaagggc cgggactgga ccgggaactc
     2761 ggtctatctc tggggcatcg ccaacgcggt ctacatgggc ctgatgggcc ccgcaggctt
     2821 ccgcgaggtg ggtgagctga tcatcgcccg ggcgcactac gcagccaagc ggctggcgcg
     2881 ggtgccgggt gcggcgatca gatggcggag cggcttcttc aaggagttcg tggtcgactt
     2941 cacgggcacc ggcaagaccg tccacgagat aaatgcggcg ctgcgcgccc acggcatctt
     3001 cggcggcaag gatctctcgg ccgatttccc ggagctcggc caggctgccc tctactgcgt
     3061 caccgagatc cacaaccagg ccgatatcga ccggctcgcc gaaacgctag aggaggtggt
     3121 gcgatgagcg ggaacgacac gaagctgcgt cgctaccagg ccccggtctg ggacgagccg
     3181 gtgatcatgg agatgagcag ccccggccgg cggggccagg tcttcacagc gcccgagccg
     3241 gcggtggccg acgcggtggg cgacatcgcg gcccttctgc cggccggcat ggcgcggaca
     3301 gcaccgccgg cgctgccgga gatgagcgag cacgacgtgc tccggcattt cctccatctg
     3361 agccaggaaa cgctcggcat gatggggatc agcctcttcg gcacctgcac gatgaagtac
     3421 aacccggcgc tgggcgaggc gctggcctgg cggccggaga tcgcggccct gcacccgctg
     3481 caggacgagg acacgctgca gggggtgctc gccatcgtcc acggcctcga ccggcagctc
     3541 cgcgagctct cgggcatgga ccagttcgtc ttccaggcgg gcggcggggc gcatgccggc
     3601 tacaccaatt gctgcgtcac ccgcgcctac catgcgtcgc ggggcgagct ggcgcagcgc
     3661 aaccagatca tcaccagcat ccagtcgcat ccctgcaacc cggcgaccgc ggcggcggcc
     3721 gggttcgagg tgatcacgct gatgatcgag gagaatggct acccctccct cgacgcgctc
     3781 aaggccgcgg tctccgaccg cacggcggcg ctgatgatcg gcaatcccga cgacatgggc
     3841 atctacaacc ccgagatcgt cgaatggata gagatcgtcc acgcagccgg cggcctgtgc
     3901 ttttgggacc acgccaactt caacggggtg atgggccggg tccgggcggc cgagctcggc
     3961 ttcgatgcct gcatgttcat gctgcacaag accttcggcg cacccaaggg cggcaacggg
     4021 ccgtcggtcg gcgcctatgg ctgcacgaag gagctcgcga ggttcctgcc gaggccgatc
     4081 gtcgtcgagg aggcggggcg ctttcggctc gaccacgacc ggcccgacag tgtcggcaag
     4141 gtgcgcgagt tctggggcaa cgtgccgcag ctcatgaagg cctatgcctg gacctgtgcg
     4201 atgggcagcg acggcatcgc cgaggccgcg gatctctcgg tgctcgccaa caactacatg
     4261 gacgcgaagc tcggcaggat cagggggctg agcaagtcgc acccgcagct caccgcaccg
     4321 cggatggaga tgacccgctg gagcctcggg ccactgaagg aggagacggg cgtcggcacg
     4381 ctggacgtgc agaaccgcat ggtcgatttc ggcgtcgatg ccttctggct cagccacgag
     4441 ccctggctgg tgcccgagcc cttcaccccc gaggcgggcg agctctggtc gaaggaggat
     4501 ctcgactact gggtcgcggt gatcgagcgg atcagcgagg aggcctacag cacgccggag
     4561 ctggtcaagt cggcgccaca caaccagacg atccaccagg tcaaatacga cgggctggag
     4621 gatcccgagc gctgggcgat gacgtggcgg gcgtacgtca agaagcggga gcggcggggc
     4681 gaggccgcct gagtcgccgg acacggttcg ccggtcgagc gatcgttaca tttcgcgacc
     4741 aatgctgcct tgccagccaa ccggccggtg cgtaccgagg tggccatacc gggtggcggc
     4801 aggcaatacg accatccggc agccctcgca aaaatggctg gtggatgcgc ccaaccaaag
     4861 gaaggctctg cggcctgatc ggcgccggcg gattcgggcg agaggtcatg ccgctgctcg
     4921 aggcctgcgt cctcgggcag ggggtgttca tgcccggtgg cgaggcggag gtcgtgttcg
     4981 tggaatcgcg gcccgtgctg gccgagatcg gcgggcggcc cgtgctctcc ttgaaggagt
     5041 tcctcgccgc ggatggcagg gacaggctct tcaacgtggc gatcgcggag tcgcgcattc
     5101 gcgaggagct ggcgctggcc tgcctcgacg caggtggtct ggcgcagacg ctggtcgccg
     5161 gcgatgcgga gatctttcca tccgtgacca tcggcgacgg ggcgatcatc tgtggccgga
     5221 cgatgatcac cgacaacgtg acgatcggcc ggttctttca ctgcaacatc tattcctatg
     5281 tcgcgcatga ctgcgtcatc ggcgacttcg tcacgttcgg gccgcgagtg agctgcaacg
     5341 gcaatgtgcg gatcgacgat cacgcctatg tcggcgccgg tgcggtcatc cgccagggtg
     5401 cgcccgggcg gccgctggtc atcggcaagg gcgccgtggt cggcatgggg gcggtggtgg
     5461 ttcgcgacgt ggcgccctgc accacggtcg tcggcaaccc ggcccgaccg ctcgaccccg
     5521 caatcctgga cggaccggag gtcctcagcg ccaccggatg aggatggcgc cggcgacgag
     5581 gcaaagagca cccaggagct tggtccagtc gaacgggcgg ggcgtgaggc ccatcaggcc
     5641 gaagctgtcg atcaccaggg cggccgcgag ctggccgcag atgacgagcg ccaccaccgg
     5701 ggcggcaccc aggcggggcg tggccatgag ggcgatggtg acgaaggccg cacccatggc
     5761 gccgccgagc cagagccagg gcggcaccag gcgaagatcg gcgagcgccg gcacgggcgg
     5821 gcggagcgcc atggcgacgg cgagcagcag cacggtggcg gtgccgaagg agacgagcga
     5881 ggcgctgagc ggatggccga gatgacgcgc cagaccggca ttgagcgtcg gctggatgcc
     5941 gagcagaatg ccgccgagga ggacgaggcc gagccagagg gtcgtcatgc gctcggcata
     6001 gcgggcggcg gagccggagg gaacgcctgc cgtgcgccgc tgcgggagat tcctcgatgg
     6061 atggcaatga agagacgggc agcgccgggc gggcagcggc aggactgaca cgcgccgagc
     6121 tcgcgaagct ggcgtcctgc ccggcggccg agctcgactg gctggtgggg ctcggcctga
     6181 tccgcccgga tccgggcggg cgcttcgccc atggtgacat ctcgcgcgtg cgcctggtca
     6241 tggcgctcct ggggtccggc ctcggccggg cgcagctcga ggcagcggca cgggcggggc
     6301 agatctcggt cgacttcgcc ggcgacatga tcgccgacgc gccggcgctg accggcctga
     6361 cccatggcga ggcgctggct gcgatcggcc tcgatgcgga cgaggcaagt cggatcgggc
     6421 gggcgctggg cctgccggcg gcggcacccg gcgatccgat ccgcgaggac gaccgcgagc
     6481 ttttggccct cgtcgccagg gcgcgacagg aaggcattgg cgaggcggcg atcctgcgca
     6541 tgctccgtgt cttcgcgctc ggcatcaggc agatcgtcga ggcgcagcgc gatctcttcc
     6601 gggagaacgt cgaggagccg ctgctcgcct cgggcatgca gcggcgcgag ctgctggcga
     6661 agacggcacc cctgcgcccg cggctgcaac ggctcggcta tcgcgcggtc ttcctgattc
     6721 tgcggcggct gctcgagcag gcggtcttcg acaatgccat ggcacggctc gacgagagcc
     6781 tggccgagca gggcatcgtg cggccggcag cggccgaccg gaccatcgtc ttcatcgatc
     6841 tttccggctt cacggccctg accgaggccg aaggcgacgc ccgggcggcg atgctcggca
     6901 gccgcttcct cgagctcgcc caggaggcag cgctccatca cgcggggcgg ctggtgaagc
     6961 tgctcggcga tggtgcgatg ctgcatttcc gccggcccga ggacgcggtc gcggcggcgc
     7021 tcgccatcat cgccggggca cccggccacg gcctgccgcc ggcccgcgcc ggcatcgcca
     7081 ccggcccggt gatcggccgc gacggcgact gcttcggccg cacggtaaac atcgccgccc
     7141 gcctcgccga cgccgtgccc gccggcggca tctggctcga tgccgcgacg gcggcccatg
     7201 ccgggagacc actcgtgccg gccggccggc ggtcgctcaa ggggctggtc gagccggtcg
     7261 aggtgttcgg gctcgaccct tgacggcacc ggcagtggcc acgtccatcc tgtccgcagg
     7321 tccagacctg tcgacttgcg aacagggcgg tcactcccgt tgatgtgcgc cggtggcgcg
     7381 ccgggcgaga cggggcacgg cgaaggtgag ggctgtggcg gcgaggaggg cgagcatgat
     7441 ggcgacgccg acctcgctcc aggcgccggg gcggacagtg ccgcaataga cgatcacggt
     7501 gaacagcggg aggtcgagga agtgcgcgac ctggcccgcc gtgctccgcg cctcgtcgcg
     7561 cagatcatcg gtgagcgggc gaccctcggc cagtgccgcg cgcgagcggc gccgtgtcct
     7621 cgtgaactgg atccgggtca cagtgtttcc ctcgatgagc tcgaacagaa agagacccca
     7681 catgccgacg agccagggct cctcgaaaaa gccgaggccg agcatgtaca cgaggaagaa
     7741 gcccgagacg ccgacgagca cggcgcccgg gacggcgagg ctgtcgtaga agagggcggt
     7801 gtagtgcgag gcctccgtga agaccgtgag gtcggatgtc cggtaggcgc gaagctcgga
     7861 gacgaagacc gctagcagcc cgccgccgag caggatgaag cccaggacat ggccgaattt
     7921 gaggagcggg taggggtcca tggcgggttc tcaggctctt gccttgccga gacggggccg
     7981 gatcacggcg acgagcaccg tggcgaggca gagcagcacg ttcaccggcc caaaggtggc
     8041 ttcgcgggct ccgagcgcat ggagctggtc gattggcagg aggccagcgg ccacttgcgt
     8101 tgcgacctcg agggcttctc gacccgtcgg gtagagcacg agggcggcgt tcagcgcgat
     8161 caggagacca aagagcgcat gcagcgtcag ccagcgtagc ctgaagatcg gcagcctggc
     8221 cttgacgatc atggaagtgc cggtgacgag cagcaggatg aggcccggca tggtcaggta
     8281 gctcgtcgcg atggcgatcg cctggcggat gacgagcgcc gtttgcgggt cgtcctcggc
     8341 gcccgggatg agtccggtcg tgacgtgggc gagaatgctg ccgaagaaga gcatggcacc
     8401 gagcagatgc agcgccttga cgagcttgag catggcttca ggcctcctcg gtgtgatccg
     8461 cctcgagtcg ctggcgaagg gtgtgcatca gggcgagcgc cgcctcgatt gtttcgatgg
     8521 ccaagcctgc agcgagcctg ttcgaccatt cgacctgccg gcggttgacc tcgttcaacg
     8581 ccaggcggcc gcggtcggtc aggtgcacga gcttggcgcg ccggtgattg ggattcagct
     8641 cgaacgtgac gagaccctcg tcggcgaggg cgttggccag gcgctgcacg gcctgccgcg
     8701 tcagccccat gtcccgcgca atctgggcga ccgggagagg tacggcctcg atggcaccca
     8761 tgacctgcca tctggcgctc gacaggccga gatcgccggt cagccggtca ccgtccgcca
     8821 gaagccggcc gttcagacgg aaggcctcga ggatcagcgc ggtcaggacg gccgcttcgg
     8881 gggtgtgcgt gctcatgaga caacatgtgg tcataatgac aatatgttgt caatatatga
     8941 tcaaggctgc gtttggcgcc tcgcatgtgg cgtgcgccgg ctctggccgg actcccctca
     9001 gagcatcgat tcgagcctcg tgttgaccac cagctcggcg acgctggcgt tgtcggggag
     9061 gctgaggagg aggctgacgg tttcggcgat ggtttcgggc tcgatgcggc cggggccggg
     9121 gacggcgccc gggacgttct ggacgagttc ggtctcgacg gcgcctgggc acagcgcggt
     9181 ggcgcggagc tggtcgtccc agccggcgaa gcgggtggcg tgggtcatgg cgaccagcgc
     9241 gtgcttgctc atgacgtagc cgagcgaggc ggagcggtcg cgcacgcgct tgccgtcggt
     9301 cgagcagatg ttgacgacgc ggccgttggc ggcggccctg aggtggggga gggcggccct
     9361 ggtcaggcgg aaggggccct tgacgttgac cgcccagagc tcgtcgaggt cgtcctcgct
     9421 gccttcctcg aaggtcacgg tcctgaagat gccggcattg ttgacgaggg cgtcgagccg
     9481 gccgaagcgc tgcagcgtgg cctcgaccca ggctcctgct gtgggtgggt cgagcgcgtc
     9541 gaagcggtgg gtcaggatgc gctcggggtc ggtcggcggc gtaggggtgc gcatgccgag
     9601 cgagaggctg tagccgtcgg tcagcaggcg gtcggcgatg gcggccccga tgccgcgcga
     9661 ggcacccgag atcatgacga cccggccatc ggcgggcagg agggccggca tctcagaaga
     9721 ggccctgata gacgccgccg tcgttgacga tgttctggcc ggtcatgaag ccggcctggg
     9781 cgctgcagag gaaggcgacg aggtcgccgc attccttcgg gtcggcgaag cggccggcgg
     9841 ggcagccctc gaggcgatcc tggacgatgg cctcgtaggt ggtgttgccg cgcttggcgt
     9901 ggccgtggag gttggtgtgc agcgcgtcgg tgtcgaagag gccggggcag accgtgttga
     9961 tcgtgacgtt cttgccgatc aggtcgcggg tcatcgaggc cacggcaccc gagagggcga
    10021 ggcgtgccgc gtggctgtgg gcgaaattga cctgcgggaa cttgatgaag ctcaccgaca
    10081 tgttgacgat ccggccgaag ccgcgctcgg ccattccggg cgcgcagcgc tggatgaggt
    10141 cgagcgagga aaagcagtgc gcctcgaacc agtggcgcca ctcgtccttg gtaatgtccg
    10201 caaacggtgt tggtgtctgg cgcacgcccg ggttgttgac gaggatgtcg ggggcgggac
    10261 aggcggccag cagcttcgcc cggtcgtcct cggcagtgaa gtcgccggcg atggcctgtg
    10321 cctgcacgcc ggtcccttcg gcgatggcgt gggccgcctc ctccagcgcc tccttggttc
    10381 gggccatcag ccagaggtcg cagccggcct cggcaagcga gagggcgcag gcgcggccga
    10441 ggcccttgct ggcggcgctg acgatggcct tgcggccctt gatgccgaga tccatggctc
    10501 aggctgcctt cttgttcagg aggccgagct cgacgaggcc gcgctcgagg ccggccaagg
    10561 gctcgccttc gagacggcgg agcggtgcgc ggggctcgcc ggcctgccag ccgagcatct
    10621 ccatggcggc cttggtggcg gggtagagcg tgcggccgcc ggtggtgaag aggtcggtga
    10681 ggcggcggcc cgtcgcctgg agggcagcgg cctcggcgcg gcgggcgggg tcctttgcgg
    10741 cttcgaagag ggcgatcccg cccggcagcc agacattggg aaagcagtcg atcaccccgt
    10801 cggcccccag ctcgacggcg ggcacgccga agacggagga ggggccgcag aagacccgca
    10861 ggcgatcggc gacggcgaga tgggtgccgt agaaattgtt ccagtcgccc gagctctcct
    10921 tgatcgccac caccttgtcg acgtcggcta ggcgtgcggc cagggccggg gggatggcgt
    10981 tcacggcatt gcccgggatg ttgtagaggc agaccggcag cgggctggcg gcggcgacgg
    11041 tctcgatgtg gccgaagatc tcgtcgtcgg tgagcttcac gaaatagggc ggcaggacga
    11101 gcacgccgtc gcagccggca tcggcagcgg cccgcgccga ggcgatggcg tctgcggcga
    11161 ggatggcacc cgtgccggcg aggatggtcc ggccctgggc cacctcgacc gcgagcctga
    11221 agagggtggc gcgttcggcc agcgtcagcg tccagaactc gccggtgcag ccggcgacga
    11281 ccgcacccgt ggcgccgtgg tcgaactgcc gctcgagatt ggcggtgaag gctttcgtgt
    11341 cgatggcacc cttcgcatcg aacggggtga tgacggccgg catggcaccg gaccaggcaa
    11401 cgctgttgcg atccatgggt tcagctttcc ttgttgaggc gccggccgtc ttcaccggcg
    11461 aagagatgca gattggcggt gcggaacgag aggccgagcg gctcgccggc ccgaagcgcg
    11521 atctcggcgc cgacccggcc ggtgagctgg tggccggcga tctcgagctg gacgatcgcc
    11581 tcgtggccgg tgggctcgac cagcttgacg cgggccggct ggcccgccgg gtcgatgagg
    11641 atgtcctcgg ggcggatgcc gagcgtgacg gccccggtcg ggcggtcgag cgcgagccct
    11701 tcgaccgaca gccgccactc gccggcgacg aaggcgccat gctcgaggcg gccctcgacc
    11761 aggttcatcg acggggtgcc gatgaagccg gcgacgaagc ggttggccgg ctggcggtag
    11821 atcatggccg gcgtgtcgag ctgctgcagc cggccattcg ccaggacggc gatgcggtcg
    11881 gacatggtca tcgcctcgag ctggtcgtgg gtgacgtaga ccatggtccg gccgatgcgc
    11941 tggtgcagct cgatgagctc ggcgcgcatg tgcgcccgga gcttggcgtc gaggttggaa
    12001 agcggctcgt cgaggaggaa gaccgcagga tcgcgcacga gggcccggcc cagcgccacg
    12061 cgctgctgct ggccgccgga gagctgccgg ggcttcctcg ccaggtagtc ctcgagatgc
    12121 aggagcttcg ccacctccgc caccttgggg ccgcgctcgg gcttcgggat gcggcgcttc
    12181 ttcaggggat attcaatgtt ctgcgccaca gtcatgtgcg ggtagagcgc ataggactgg
    12241 aagaccatgg cgatgtcgcg cttgcccggg gcgatgtcgt tgaccacgcg atcgccgata
    12301 cggatcgtgc cggaggtcgg cacctcgagc ccggcgagca tgcgcaagga cgtggtcttg
    12361 ccgcagccgg aggggccgag caggctgacg aactcgccgg attcgattcg caggtcgagg
    12421 ctctcgacgg cgaccgcggc accgaacgcc tttcgtacac cttcgaagac gacctcggac
    12481 agggctctag cctttcacgc cgcccgcgcc gaagccgcgg gtcaggtatt tctgcaggaa
    12541 ggcgaaggcg acgatcaagg gcaggctcat catgacggaa gcggccatca aaagggacca
    12601 ttcgatgttg aaggcggaga tcagcatggt catgacgccg gtcgtgagcg gcctggcgct
    12661 gtcggtgttc accagcacca gcgcgtagag atactcgttc caggcgagga tggcggtgaa
    12721 gagggcggtc gagatgatgc cggggagcgc ctgggggagg atgacgtcga tgaaggcttc
    12781 catgcggctg gcgccgtcga ccagcgccgc ggcctcgagg tcgtcgggaa tgccggccat
    12841 gaaggagcgg agcagccaga gggcgtaggg cagggcgaag gtggtgtagg cgaggatcag
    12901 gctccagagc gtgttggaga tgccgatctt caccatcagc agatagagcg ggatgatcag
    12961 cacgaccgat ggcaggaggt aggtgaagag gacgagctgg gccagccgct cgcggcccgg
    13021 gtagcgaaag cggacgaggc tgtgggcgcc gagcgtggcg agcgtgatga cgaggagcgt
    13081 ggtgccgagg gcgaggaaga tcgagttgcg catgtaggtg aggaagggcg tctcgaccag
    13141 gagccggcgg tagtgctcga aggtgatctc ggtcggccag aagctcggcg gaatgcggaa
    13201 gagctcggtc tgcggcttga ccgaggtcag gatcatccag atgagcggga aggcgaccag
    13261 gaggacgagc gtccatgcca ggagattgaa ggcgatccgg ccgggaagcg aggtccggcg
    13321 catcaggaat tgccctcgcc tctggccgtg cggcgcagat agaggatcat gaagaccagc
    13381 atgaccagca tcatgaggac ggcgacggcc gacgcgccgc ccatctggtt gagcgcgaag
    13441 gcctccttgt agaccatgag cgacagggtc tgggtcgaga tgaccgggcc gccgccggtc
    13501 aaaagataga tcaggtcgaa ctccttgaag tcccagatgg cgcgcagcat gacgatgacg
    13561 acgagcacgt cgcggagctg cggcagggtg atgtcgagga agcgggcgat gggcccggcg
    13621 ccgtcgatct cggccgcctc gtagagcgcg tcggggatcg actggagcct cgcgagcaca
    13681 gcgatcacga cgaacgggaa gtacttccag gcgccgacga ggatgatgct gagcatggcg
    13741 ttcggcatcg agcccagcca gtcgagcggc atgtcgagca cgccgacacg catcagcgtg
    13801 tggttgagga tgccgtagag gtcgttgaag acccagcgcc agacgaggac ggcgaccacg
    13861 gtcgagacga agtaggggaa gagcacgagg ctgcgggcga gcgggcggaa gaggaggctc
    13921 tggttcagga ccagcgccat ggcgacgccg aggacgatct gcagcgtgag cgtgccggcc
    13981 gtccagacca gggtgttcct gagcgagatc cagaactcgc ctttcgccag gaggcgctgg
    14041 tagttggcaa ggcccaccca ttcgccctgc agggtcggcg tgtagatcga gaagaggctg
    14101 agatagatgg cggagagcaa gggatagacg atgaccgccg tgaagacgag gacggtgggc
    14161 gcgatcagca gcaagcccag gatgctgtac tgggtatcga gggagagacg tcgcgcaggc
    14221 gtggcgctgg ccatcgtccg gttgctcagt cgaggccgag cagggctgcg gcgttgtcgt
    14281 ggagcacgcg ctcgagcacc gattccctga tgggcagggc caaaaagcgg gccagatagt
    14341 cgtcgagcgg gcagaacgga taggcggtgc cgaaaaggaa gcggtcggcg aggaagccgt
    14401 tgatggcggt cacgtagtcc gccgtgccgg gcaggccgtg caggtacatg tccggcgaga
    14461 ggtagagatt ggcgcggcgg aaggccacgt ggatgatctc ctgcacccag ggccagttgc
    14521 cgtgcgaggc gacgatcttc agcgtcggga aatcgcccag cacgcggtcg atgtgctcgg
    14581 gcgcggtgta gctgatatcg ggtccggcag cgccgccgct catgacgatg agcacgatgc
    14641 cctggtcctc gcagtgggcg tagatcgggt acaggcggcg gtcgtccgag tgcaaaggcg
    14701 gtgccgcgac gcccggctcg atgttgatgg ccttcacacc gagcgccacc gcctcgtcga
    14761 tcgcctgcat cgccgccttg cggttggtcg ggtcgatcga gcccaccggc acgaagcgat
    14821 cggggtaacg ggcgcacatg gcgacgacgt cgtcgttcgg gatgacgccg agcgtcgggg
    14881 agttgcggcc cacgaccacg cccctggcga tgcccgcggc gcccatctcc tcgagcagga
    14941 gatcgatcga ctgccgctcc gccgaggccg gcggctcgaa gccgagcgag cgtgtgtagc
    15001 ggttgcggtt gtcggcagcc gcatagatgc gcgattgcag gaagctcccg agcggcgggc
    15061 gcatccgaaa gtcgatgatc ttcaccgcag tctccaagca gctcggccga aggatattgc
    15121 cagggtgacc ggcgtcagcc cttcatgatc tcgtcgatgc gctgcgccgt ctcgccgacg
    15181 accgagcgcg catcctcgcc gttgacgcag acgcgctgca ccatctcggc gagcacgagg
    15241 ctgttgacga cctcgccggc cttggcgttc ggttgatggg cgggcgattc ccaaccgaga
    15301 ttgtaccccg cggcggccgc ctccgacatc agctccacct cctcgggata gcgctggatg
    15361 atcgggttat ccagatagcg cggatcctgg ccgatcgtcc gcaggaccgg cagcagatga
    15421 ccgggcgcgc catgcagttg cctgatgtag ccttcgggct cgaagaggaa ggcggcgaac
    15481 agcttcgctt cggccttgtt gggcgcgtcc ttgggaatga agacgctcgg gaagccgttg
    15541 aacgtccagg gctcgacgtc ggtcgagacc cgcgggtagg tggcgcaggt gatggcatcg
    15601 gcgatggcgg ggttctgggt caccacgttc tgcaggacac gtcccgcata gatggcggtg
    15661 gcactggcgc cgctgacgaa ggcacccagg ctctcgcccc agctgtagcc cgtggcaccg
    15721 ggcgggcaga gctcgcgcat cgacttgtag aactcgagcg cgttcagcgt ctcgtcgctc
    15781 tccagcgcca cctcgagatc gggcgagaag acctgcccgc cggcgagatg gatgaagccg
    15841 atgatcgtca gactcgtcat gccgttgcgg gcgaacggca gcggtgcccc gtaaacgccg
    15901 ccgccctgca tcgcctggca cgcggccctg agctcgtccc aggtcaccgg gatgtcggcg
    15961 atgccggcct gctccatgag atcggtgcgg acccagagca tgttggcggc agcgttgccg
    16021 atgccggtgc cggcatactg gcccttgtcg atctcgtaga tgcggttctg gccgggatag
    16081 aacttctccg gcccgatggc ctcgatgacc tcgttgaagg gctccagcag accattgcgc
    16141 cagtagttgg aaacggcgaa ggacggtaga tgggtgacga tgtcgggcag ctcgccgctg
    16201 gcgaaggcgg cggcgagctg cggcgcgtag ccctcgtcgg aggtcttctc gaagaccacc
    16261 ttgacgcccg gatgggcggc ctcgaacgcg ccgatctgga gctcgtaggc ggccacctgc
    16321 tcgggcgagg cctgcgccgc ccaccagcga acggtcgtgt cctgggcgcg agcccggccg
    16381 aatggaaggg cggcagcgag gccaagggca gagctgccgg cgagcatccg gcggcgggtg
    16441 gtcgacgcct cgacgatggt cattgcctgt ctcctcccgg gctactcgat cgcggcagcc
    16501 tcgtttgcga acaactgttc gcagttatcg gagtgacgtt ccgcgtgtgc aacagcaaag
    16561 agccggagcg gaccgatcga cgcccgacga tagcccgagg cagcatgccg tcgaggctgg
    16621 gctcggtacg agcaaggtca ggcaggagga cgagctggat gaccggaagg ccccgagcat
    16681 gccccggcgg gcgtccagcg agggtcggct gagaagggaa cgaccctccc ggacgggcgc
    16741 ctcgggcatg ctcggtacgg acgtacgtat caggctttca tgatgtcgtc gatacggctg
    16801 gcggtttccc cgaccacagt cgcaatatct tcgtcattga ggcaaacgcg ctgcaccatc
    16861 tcggccagca cgttgctgtt gacgatctcg ccggccttgg ggttcggctt gtggtcggcg
    16921 ctctcccagc cgagattgtt gccggcagcg gcggcctcgg ccatgagctc gacctcggcc
    16981 gagtatttct ggatgatcgg gttctcctgg tattgcggca tgggggcgat ggtcctcagc
    17041 accggcagga cgtggccggg ggccgccagg agctgcttga tgtagccttc gggctcgaag
    17101 aggaaggcgg cgaaggcctt gctctcggcc atgtttggcg cggccttggg gatgaagacg
    17161 ctcgggaagt cgttgaaggt ccagggtggc acgtcggtgc tgatgcgcgg gtaggtggcg
    17221 caggtgatgg catcggcgat gcccgggttc tgcgtggtga cgttctgcag cacgcggccg
    17281 gtgtagatgc cggtcgccgt ggcgccgctg acgaaggcgg tcaggctctc gccccagctg
    17341 tagccggtgg cgccgggcgg gcagagctcg cgcatcgact tgtagaactc gagcgtgctc
    17401 ctcgtctcgt cgctgtcgag cgccacctcg agatcgggtg tgaagatctg cccgccgccg
    17461 aggtggatga agccgatgat ggtcaaggag gtcatgctgt tggcgccaaa gggcaggggc
    17521 gcgccgtaga tgccgccgcc ctgcatggcc tggcaggcgg cccgcagctc gtcccaggtc
    17581 accgggatgt cggcgatgcc ggccttctcc atcaggtcct tgcgcaccca gagcatgttg
    17641 gcggcgctgt tgccgatgcc ggtgccggcg tactggccgg ggtcgacctc gtagatgcgg
    17701 ttggcgtcgg gatagaattt ctccgtcccg atcgcctcga tgacctcgtt gaaaggctcg
    17761 agcaggccgt tgacccagta gttcgaaacc gcgaaggagg gcaggtgggt gacgacgttg
    17821 ggcacctcgc cgctggcgaa ggcggcggcg agctgtggcg cgtagccctc gtcggaggtc
    17881 ttctcgaaga cgaccttgac gccgggatgg agggcctcga aggcggcgat ctggaactcg
    17941 taggcggcga gctgctcggg cgaggcctgc ggcgaccacc agcggatcgt cacgtcctgg
    18001 gcgtgcgccg ggcgcagcgg cagggcagcg gcgaggccca tggccgacgt gccggcgagc
    18061 atgcggcggc gactgaacgg cgttccgacg attgtcatcc tgtggctcct ccctggattg
    18121 gcgctcgcag cgatttgctt tgcgaacaag cgttcgcaat tatcgggggg aggatggccg
    18181 tgcgcaacgg caaagacgtg gaaggatcgg cgaggggcat ggcggagaac ggcgacgatc
    18241 gggatttcgt caactcgctg gcgcgcgggc tcgaggtgat ccgctccttc acccgtgcca
    18301 ggccccggat gacgctgagc gaggtggcgg ccgagaccga cctgccgcgc gccgccgtgc
    18361 ggcggttcct cctgaccctg gtgcgcgagg gctatgcggc gaccgacggc aagctcttcc
    18421 agctccgccc gaaggtgctc gagctcggct attcggtgat cgcgtccttg agcatcgtcg
    18481 aggtcgcgca accggtgatg gccgatgtct cggccaggct caacgagagc tgctcggtgg
    18541 cggtgctcga cggcgacgag atcgtctatg tggcgcgcag cagcgccaag cgcgtggtga
    18601 acatcgccgc caccatcggc ggccgcctgc cggcctatgt cacctcgatg gggcgggtgc
    18661 tgctggcggc ccaggacgat gcggcgctcg acgcctatct cgcccggatc tcgcccgaga
    18721 agctgaatgc ccggacggtg acgagccggg tgaagctgcg cgacgccatt ctcgaggcgc
    18781 ggctccaggg ctggtcgctg gtcgaccagg agctggagcc cgggctctgc tcgatcgcgg
    18841 tgccgctcag ggaccgcgcc gggcggacgg tcgcggcgct cgccatcggc gcaccctccg
    18901 ggcgggtgga cgccgcgacc atgcgcgagc gcttcctccc cgtcctcctg gaggcggccc
    18961 agcagatggc catggccatg cccgattagc ggatggtcag aaggctggtg ccttggtgcg
    19021 gaaccggtcg gcggtgtagg cctcgaggtc gagctccggc cgccggccga gcatgtggtc
    19081 ggcgacgagg cgggcgacga gcgggccgag cgtgtagccg gcgtcgcccg gcagcgccac
    19141 gtagaggccg ggtcgcccgg ggaccgggcc gagcaccgag cggccgtcgg tcgtcgtgtt
    19201 gatcccggcc caggagcgga gcagccgggc gcgcgccacc tccggcacga tatgcaccgc
    19261 gaggccgaga ttgccggcga ggctggtgcg cagcacctcg ggcggccggc cgcccttgcc
    19321 ggcccgcgcc ggccagccgc cgccgatcac gagctggccc gtggcgagct gcttcatcgt
    19381 gatcatccgc tcggcatgct ggaccaggtg gccgatcagc ggcgccgcgg gctcggtgac
    19441 gttcatgtgc aaaggctcgg ccgcggtcgg cactgccacg ccgagcggtg ccagcagggc
    19501 gcccgtgccg gcaccggcgg cgatcaccag ccgccccgcc cggaccatgc cggcggtggt
    19561 ccgaagggca tagccggcgc catccggcgt gatgccgagg acgcgggtgc cgcgctgcag
    19621 ggcgacgccg gcggcccggg tcgcggccgc gatggcggtg ttggcggcga gcggatcgac
    19681 cttgccctcg ctggcgcagc gctcggcgcc caccacggcg gggcccaaat agggggcgat
    19741 ccggtcgagg gcccggcggt cgaggatctc gacctcgagg ccgagggcgc gctcgcgcgc
    19801 cgccttggcg cgc
//
LOCUS       DAOTFI010000071        19687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3326_length_19687_cov_7.025570, whole genome shotgun sequence.
ACCESSION   DAOTFI010000071 DAOTFI010000000
VERSION     DAOTFI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19687
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3326_length_19687_cov_7.025570"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1104)
                     /locus_tag="P5558_09100"
     CDS             complement(178..1104)
                     /locus_tag="P5558_09100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY24505.1"
                     /translation="MMADRGRDVAVPVPRVQRMDAGEPLGRLMAALFGLVERLGWQRA
                     TLLCTLTFTIAAAVMTVALKSLLGVPPVALSLDLLVVAAVTVLLAGPGLAVALRLVEH
                     LGRARARLLVEIDRRMVAEQQLRRLATTDDLTGLSNRRHFVERAREAMAIAQRYDQWV
                     TFAVVDVDRFKHLNDRFGHAAGDRALVVLAAVLRANLRATDLAARFGGDEFVLLMPLT
                     DPKAGRVAAERIRRAVREDCGRPPFRVSVGIASAKGPVAALEALMARADQALYDAKRT
                     GRNRIGICAGPTVGPDDATMDGLDRIALPLKG"
     gene            complement(1274..1855)
                     /locus_tag="P5558_09105"
     CDS             complement(1274..1855)
                     /locus_tag="P5558_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRY24506.1"
                     /translation="MSQPNSVTENEVFFRHWLRSPKSMGSIIPSSRVLARALAGAAAW
                     QPGDHVVELGGGTGAITQGLLERGIPPANIVVVELETNLHQYLAGRYPEVNVIQGDAT
                     RLAEITARLDIQRISTVISGLPMVGMPPAFKKAIVDQAFTVMPAGHFMLQYTYSPVPP
                     VPVDELGLEAELARYVLWNVPPAAIWRYRRKSR"
     gene            complement(1991..6931)
                     /locus_tag="P5558_09110"
     CDS             complement(1991..6931)
                     /locus_tag="P5558_09110"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRY24507.1"
                     /translation="MVLRGEEQKQELILAVIAEIEAKLTGEEAAQAAAFVRTYYRDVA
                     PADVLSREVQDLYGAALAHLRQAGQRPAGTLKVRVFNPGIAQHGWQSSHTVVEIVNDD
                     MPFLVDSVAMELSRHGLGMHLVIHPIFAVERDAAGRLEWIGDREGAPESARLESFMHI
                     EIDRQSDADVLEALEGDLVRILGDVRKTVVDWRSMRERVGDMLAELEPAAQHLPADDL
                     AEIKAFLEWLADDHFTFLGYSAYALEGEGTSLLLRRVKGSGLGILRAHDDGAPSKSFM
                     EMPLAARLRATEPLPAIAITKANTKSTVHRATYLDFIGVKRYAPDGRVIGEHRLLGLF
                     TSVAYNRSPRFIPLLRRKFDAIVERAGHVRAGHAGKALVNILETYPRDELFQADEDTL
                     FEITSQILYLQDRQQIRLFVRQDTFGRFVSCLLYIPRERYNTTLRERLQSLLLEAFDG
                     SDSEFQAQVTEAVLARIMVIVRTPEGVPDDLDVEALERQIQELSQSWTDRLHAALIEA
                     EGEEEGNRLYRTFSRAFPASYQERNAARAAVPDILVMDRLEQAAGDRLGMTLYRRLED
                     SPEMVRFKLVRRELPLHLSDALPILENMGLKVLHEEAHPVRNARGKLFSMHDFGMRPV
                     SGAVVDVDAVRDAFQEAFERIWSGAVENDGFNRLILAAGLDAAAVVVLRAYCKYMQQL
                     GTPFSQAYIENTCTTNPALAADLVALFNVRFDPDFAGDRAARTVEIEARIKTGLEAVA
                     ILDEDRILRRYLGLIHATTRTNAFQRTAEGGPKAYLSLKLDPARVPEMPKPRPAFEIF
                     VYGPAVEGVHLRGGKVARGGLRWSDRREDFRTEVLGLMKAQMVKNGVIVPVGAKGGFY
                     VKRPPETTERQVIVDEAIRCYKLFLSGLLDITDNQVKGAVVPPPRVVRYDDDDPYLVV
                     AADKGTASFSDVANAVSRDYGFWLDDAFASGGSAGYDHKGMGITARGAWESVKRHFRE
                     MGLDPAVDLFTVIGIGDMSGDVFGNGMLLSDRIRLLAAFDHRHIFLDPDPDPASSFAE
                     RRRLFALPRSSWDDYDRSLLSAGGEIVSRRAKSVTVSPQVQAALGLDRDTWAPTELIS
                     AILEAPVDLWWNGGIGTYVKASSESHHDAQDRANDGLRIDGRELRCKVLVEGGNLGMT
                     QKGRIEAASSGVRLNTDFIDNSAGVDCSDHEVNIKILLGEVVAAGDLTMKQRDALLAR
                     MTDEVAALCLRDNTLQNLALSLTEARAADFAEPQARLMRKLERAGRLDRAIEFLPDDA
                     ELAERRRAGRGLTRPESAVLLAYAKMTLFTDLLATELPDRPYFAGDLAKYFPRPLRRG
                     FAGPIAEHRLRREIIATWLANSLVNRGLDVFCTELEDETGASLADICLGYVITRDAFA
                     LLPLWAAIEDLPREVPAALQIEMLGEARETLVRGTRWFLAHTPRPMRIGNTVATFAAP
                     IATLAGALESTLAASQSGELASTIALHVEAGVAPELARTVAALPYLLAGCDIVTVTRE
                     LAGSATGPGVENGDPAGDAPVAGGVVDDPLPVARLYFALDEGLSLSRLRQRMRQAPIR
                     TRWDRMALSGLEDELSMALRALTLAAWHSGIRAETPEAAASRIAAWLDATLDGLGRYR
                     ELLAEIDSARDPDLAMLSVVVNSAGKLLRNVVLAAA"
     gene            complement(7029..7841)
                     /locus_tag="P5558_09115"
     CDS             complement(7029..7841)
                     /locus_tag="P5558_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24508.1"
                     /translation="MAERLLEGQVALVTGAGRGLGRAFAERLAALGCAIGVHGRREHG
                     PAEYGEGTTLTATAEAIAAGFGVPTLAVLGDLTDGAVVERVVDTVAERLGPLDILVHN
                     AGGDIAAAGGKPDPNDAVWVKEADVRAVLDSNLLSTILVCRAVAQRMIPRNRGRILTI
                     SSIAAFKGREQGAIYATAKAGVVHYTRCLAQQLRPHDIAVNSLAPGDTRTGRYLGTRA
                     VDQGRLVEAGTLRRIATVDEVARVVELFAGPLGAFVSGQVLRVDGGEQSWPG"
     gene            8010..9596
                     /locus_tag="P5558_09120"
     CDS             8010..9596
                     /locus_tag="P5558_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY24509.1"
                     /translation="MKFAAARTGLLALALLSGTSTLASADVVLRLGNDTDPATLDQHK
                     TSTVAESRLLRDLYEGLVAEDAAGKIIPGSALSWEISDDGLTYTFHMREDARWSNGDP
                     VTADDFLFTFRRIMNPATAAGYASVLFPILNAEAVAKGERQAGDLGVAAPDPQTLVIT
                     LNAPTPYFLQLLTHQTGLPLHPATVESFGDAFTKPGNLVSNGAFVLESFVPNDKIVMR
                     KNPQFHDAANVQVDEVQWIPFEDRSACLRRFEAGEVHTCSDVPAEQMKYMKENLPEAL
                     RIAPYLGTYYLPVKVAKPEFADPRVRQAISMLIDRDFIADEVWQGTMLPAYALVPPGI
                     DNYVENPPELDYAKADLLDREDAAIALLQEAGVDPATLDIQLSYNSSENHRNTMAAIS
                     DMLSNVGIESSLNEMEGTGYFNYLREGGDFDIVRAGWIGDYSDPQNFLYLNETGVSFN
                     YSKWSNEAYDAKMRAASQETDLDERAKLLAEAEAIYLAEVPTIPILFYSSRALVSDQI
                     SGYEDNLLDHHATRWLSLNQ"
     gene            9655..10578
                     /locus_tag="P5558_09125"
     CDS             9655..10578
                     /locus_tag="P5558_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY24510.1"
                     /translation="MLTYTLRRLASAVPTVLVIITLTFFMIRLAPGGPFDLERPLDPL
                     IMANLEKAYNLDAPLWSQYLTYLGNLLHGDLGPSFTRRDFSVNELFAVGMPVSILLGG
                     LALVTAVVLGTLLGAWAALRQNTGVDYAVVGLATFGITTPTFVVAPLLSLTFGVVLGI
                     LPAGGWSNANPAYWVLPIVTLALPQVAVIARLVRGATIEALRSNHVRTARAYGLPTRI
                     VVGVHALRAAMLPAVSYLGPTAAGLLTGSVVVETIFGIPGIGRYFVQGALGRDYTLVM
                     GMIVVIALFIIVFNLLVDLAYAWLDPRVRFD"
     gene            10583..11710
                     /locus_tag="P5558_09130"
     CDS             10583..11710
                     /locus_tag="P5558_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY24511.1"
                     /translation="MAVQPDLIEDIPAVAGRSLWQDAWARLRRNRAAVASMIVLAALC
                     LVGIFGPMVWPHPYDQVYPKFVRVPASLEAYPGTDSILPGFEGLLQRARLEHGEPVLE
                     GGEVSVAVAADEPIDPRILRYFERSDLFSNPRLELADDGLSGTLAVDVERLYFYFGTD
                     AIGRDLAARIMIGVRISLAIGILASIMALVLGVTYGAVSGYLGGRVDNVMMRVVDILY
                     SLPFIFFVILMVVFFGRSLIIIFIAIGATEWLDMARIVRGQTLSLKRQEFVQAAEALG
                     ATKTGILMRHIIPNTLGPVVVFVTLLVPKAILLESLLSFLGLGVQDPLTSLGLLISEG
                     AANMRGALWLLIWPALTLTLILFALNFLGDGLRDSLDPKDR"
     gene            11712..13325
                     /locus_tag="P5558_09135"
     CDS             11712..13325
                     /locus_tag="P5558_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24512.1"
                     /translation="MEPVLSVRDLHVEFETNDGMVQAVRGIDLDVMPNESVAIVGESG
                     SGKSQTTMAIMGLLASNGRAMGSARYRGTEMLGLRESEINRIRGDKITMIFQEPMTSL
                     DPLYRIGAQLAEPLRFHRGLTRSQARPRIIELLRLVGIPEPERRIDAYPHELSGGQRQ
                     RVMIAMALACDPDVLIADEPTTALDVTVQAQILELLAGLQARLGMAIVFITHDLGIVQ
                     RFADRVYVMRAGEVVETGETARLFARPSHPYTKMLLAAEPEGTKAPPPAEAPDLMTGQ
                     DVRVVFRSGGGWFSKAHELRAVDGVSVRLRQGQTIGIVGESGSGKSTLGRALLRLLPS
                     QGRIEYLGRPLDPDPATMRPMRRELQLVFQDPFGSLSPRMTVGRIITEGLLIHEPGLA
                     ARERDRRAGEALTEVGLDPAMRNRYPHEFSGGQRQRIAIARAMILKPRVIVLDEPTSA
                     LDRSVQKQIVDLLRDLQAAHGLSYLFISHDLAVVRAVADYIIVMKGGRIVEEGPTAAI
                     FDHPEMDYTRTLMAAALDSSRFRESAVDG"
     gene            complement(13354..14292)
                     /locus_tag="P5558_09140"
     CDS             complement(13354..14292)
                     /locus_tag="P5558_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24513.1"
                     /translation="MRALLPAVGGRPLAELPRGLYAYVMAASRRQQVPLVLLTLAIFP
                     LTLAPIDLQRRLIDDAIGGRNPRLLLILAGLYLAAVLLQAGLKLVRNIYQERVAQGIT
                     RRLRRRIIEDGDGLADGTAQSILVAEAEKLGGFVGEAVAFPLLQAGIVVSVAGYMLAV
                     DPAVAIVALGLFVPSIVAVALMQPRLDRLAGRKTEATRELGDAALDDDRSEAAETRLD
                     RLVETIYGLRIRFAVVKFALKSFNNLLGHLGPLSILAIGGWLVLEGSTTVGTIVAFAS
                     GYQRMTDPARDLLNFYRRLSLMRVQYRMVREAAEGL"
     gene            complement(14424..15077)
                     /locus_tag="P5558_09145"
     CDS             complement(14424..15077)
                     /locus_tag="P5558_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24514.1"
                     /translation="MRIILLAAAAFTFTNVAGAAEPTRLDAAQLDWITASTAAVRALQ
                     EARPSGGDVVDGHATVEAARHELETLVGEGAAATAQPKPAGGTTVATLEDSRTVTSSG
                     GGGTIAVISGTGPGTAGAFASQTITSSSDGNGAPVVTTRTGGFATGGLTGRTFTFAPT
                     TAPTAAPAGPPVTAPTTTVSLTPLSALDGFSSRAAMPAMPTMPTMPTIPSFSVGSGG"
     gene            15238..15897
                     /gene="rpe"
                     /locus_tag="P5558_09150"
     CDS             15238..15897
                     /gene="rpe"
                     /locus_tag="P5558_09150"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRY24515.1"
                     /translation="MSPDVLIAPSILSADFARLGAEVAAVDRAGADWIHLDVMDGHYV
                     PNITFGPAIVRAIRKSSAKLFDCHLMIEPADPFIAAFAEAGADLVTVHAEATTHLDRS
                     LQLIKSLGKQAGVALNPATPATAIEHVLDRLDLVLVMTVNPGFGGQAFIPAMLEKIRR
                     VRALTAGRGIRIEVDGGINAETAPEVIAAGADVLVAGSAIFGQPDHAAAIAALRPTAV
                     A"
     gene            complement(15905..16039)
                     /locus_tag="P5558_09155"
     CDS             complement(15905..16039)
                     /locus_tag="P5558_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24516.1"
                     /translation="MTERARRAGLWLLGLAGVVALALAWRGYGDASLILMLQSLPFCG
                     "
     gene            complement(16036..17250)
                     /locus_tag="P5558_09160"
     CDS             complement(16036..17250)
                     /locus_tag="P5558_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY24517.1"
                     /translation="MTRGSARDSVAALLVCGAVIVALSLGIRFTFGLFLQPVSMANGW
                     GREVFGFAMAAQNLVWGLAQPFAGMAADRLGARRVILTGAVLYVLGVALMAQPLGAPA
                     FLVAAGLLVGLGLAGTTFPIVFGAVSRALPPERRSMAFGLTMAVGSAGQFVMLPLALG
                     LLDGLGWAAALLALAALSALMLPLAAMLGADRGNAGQAPGPSAGAAFRTAMAHRGFWL
                     LALGFGVCGFQVTFIATHLPAFLGDRGIDASVAATVLALIGLVNVVGTLAAGWLGGRF
                     AKPGLLAWVYLGRAAAIALFVYLPITAGSAILFGVLMGLFWLSTVPLTNGVVVAMFGA
                     RNMSMLGGVVFLSHQVGAFLGGWLGGVAYDRLGSYDLAWGMAIALSLIAALLNLPIRE
                     RPVTAEAARAGA"
     gene            complement(17500..18708)
                     /locus_tag="P5558_09165"
     CDS             complement(17500..18708)
                     /locus_tag="P5558_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRY24518.1"
                     /translation="MLQRTSWAWVSGAFLVGLAAGGPAEAGGLYISEFATPSMGTASA
                     GAQAWADNASTALHNPAGMTRLEGSALMVGAGFGLTEVKFDPDRSAIQGNDGGDAGTL
                     APLATLAGVLELDERWAVGLSAGGITGAALEYDDDWVGRFQVTDVSLVVAGITPSVAY
                     AVTDRLSLGVGATLWYANLNETIRPSAVGGNARVRVEDADDTDVTFNLSALFELTDDT
                     RFGVIYNSEAKLNLSGDVDVRPLGQQASAGLDFTFPQYVKASVYHDLSDSVALLGSVR
                     WEDWSAFGDIPVSADRGSTAIPANWRDTYGFSLGVHYRPVDDLLLQAGFGYDTSPVSD
                     RNRRADLPVDRQYRYSVGAQYAVSERVTVGGSLVYADLGDADIDADLFGGSYKENRAL
                     FFALNLGYRF"
     gene            18930..>19687
                     /locus_tag="P5558_09170"
     CDS             18930..>19687
                     /locus_tag="P5558_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24519.1"
                     /translation="MSLALLRRSLLASALAGLLVAPLPAALVAPTATAQVPPDVLVVG
                     QIAEPASMDPHVVTAVNDFRILVNVYDGLVRYRPGTLEVEPALAESWEISEDGTTYTF
                     KLRDGVTFHDGTPFDAAAVKFNFDRMLDESHPQHGTGPFPLSFFFSAIQETEVVDPLT
                     VRFHLNEPYAPFLSNLAYPTGLIVSPTAVETHGPDYARNPAGTGPFAFRVWESNRQVA
                     LERNEDYWDGAPPLRGVVFRPITDANARVSEMLSG"
BASE COUNT         2993 a         7124 c         6724 g         2846 t
ORIGIN      
        1 cgccggattg atcgggctct cacaactctg ttgcggcgcg cttctcgttg tggtcgacac
       61 ggcacgacgc aacgggtcgc atcggctcgc cgacccaatc cgcccatctc gcaggtaaag
      121 cgtcgaaccg tcaagtgacc gcggcccgtt tggccccggc cccaggttgg cgatcggtca
      181 gcccttgagc ggcagggcga tgcggtcgag cccgtccatg gtcgcatcgt cgggcccgac
      241 ggtggggccg gcgcagatgc cgatccggtt gcggccggtg cgcttggcgt cgtagagcgc
      301 ctggtcggcc cgcgccatca gcgcctcgag ggcggcgacc ggccccttgg ccgaggcgat
      361 gccgacgctc acccggaatg gcggcctgcc gcagtcctcg cgcaccgccc ggcgaatccg
      421 ctctgccgcc acccgcccgg ccttgggatc ggtgagcggc atgagcagga cgaactcgtc
      481 gccgccgaaa cgggcggcga gatccgtggc gcgcaggttg gcgcgcagca cggctgccag
      541 cacgaccagc gcccgatcac cggcggcatg gccgaaccgg tcgttgagat gcttgaaacg
      601 atcgacatcg acgaccgcga aggtgaccca ctggtcgtag cgctgcgcga tggccatggc
      661 ctcgcgggcg cgctcgacga aatggcgccg gttgctgagc cccgtcaggt cgtccgtggt
      721 ggccagccgc cggagctgct gctcggcgac catccgccgg tcgatctcga cgagcagccg
      781 cgcgcgcgcc cgccccagat gctcgaccag gcgcaacgcc accgccaggc cgggcccggc
      841 gagcagcacc gtgaccgccg ccaccaccag gaggtccagc gacagagcta ccggcgggac
      901 accgagcagc gacttgagcg ccacggtcat caccgccgcc gcaatcgtga acgtcagggt
      961 gcagaggagc gtcgcccgct gccaaccgag gcgttcgacc agaccgaaca gcgccgccat
     1021 gagcctgccg agcggctcgc ccgcatccat gcgctgaacg cgcggcaccg gaacggcaac
     1081 gtcgcgcccg cgatctgcca tcatgggtcc ctcgtctccg tcccgcgccg ccacgggcga
     1141 tcaggccacc ccggccccag gtcgccctga caactattgc gcgggagttt gacgcatcac
     1201 gcgcaaagcc gcaatccaca tcgcgcagat ttgaagccgg cgcgcgctgt cggccaggcg
     1261 acgaagagcg ctgctagcgg ctcttgcgac gataccgcca gatcgccgcg ggcgggacgt
     1321 tccagagcac gtagcgggcg agctcggcct cgaggccgag ctcgtccacc ggcaccggcg
     1381 gcaccggcga ataggtgtac tggagcatga aatgccccgc cggcatcacc gtgaaggcct
     1441 ggtcgacgat cgccttcttg aaggccggcg gcatgcccac catcggcagg cccgagatca
     1501 ccgtcgagat ccgctggatg tcgaggcgtg ccgtgatctc ggcgagccgg gtggcatccc
     1561 cctggatgac gttgacctcg gggtagcggc cagcgagata ctggtggaga ttggtctcga
     1621 gctcgacgac cacgatgttc gccggcggga tgccacgctc gaggaggccc tgggtgatcg
     1681 cccccgtgcc accgccgagc tcgaccacat gatcgcccgg ctgccaggcg gccgccccgg
     1741 cgagggctcg cgccagcacc cgcgaggacg ggatgatcga tcccatcgac ttcggcgagc
     1801 gcaaccagtg ccggaaaaag acctcgttct cggtgaccga gttcggctgc gacatttcag
     1861 ttccttcttc ccgcacgctg cgggggcaat ttcccagaca ccgtcgcgag ctgcgaatgg
     1921 ctggcgccag cggttcccca tgcgatcagc ggtcgtggac cacgacgtgg acgtgtaggt
     1981 aggcaaactg tcaagcggcg gcaagcacga cgttccgcag cagcttgccg gcgctgttga
     2041 ccacgacgct gagcatggcg agatcgggat cccgggccga gtcgatctcg gccaacagct
     2101 cccggtagcg gccgagcccg tcgagggtgg cgtcgagcca ggccgcgatc cggcttgctg
     2161 ccgcctccgg cgtctcggcg cggatgccgg aatgccaggc cgccagggtg agggcgcgca
     2221 gcgccatcga gagctcgtcc tcgagcccgc tcagggccat gcgatcccag cgggtgcgga
     2281 tgggcgcctg ccgcatgcgc tggcgcaggc gcgacaggct aaggccttca tcgagggcga
     2341 aatacagtcg ggcgaccggc aggggatcat cgaccacccc ccccgccacc ggcgcatcgc
     2401 cggccgggtc gccgttctcg acacccggcc cggtggccga gccggcgagc tcacgggtca
     2461 ccgtgacgat gtcgcagccg gccagcagat agggcagggc agcgacagtg cgggcgagct
     2521 ccggcgccac cccggcctcg acatgcagcg ctatcgtcga ggcgagctca cccgactggc
     2581 tcgccgcgag cgtgctctcc agggcacccg ccagcgtcgc gatcggcgcg gcgaatgtgg
     2641 cgacggtgtt gccgatccgc atcggccgcg gcgtatgggc gaggaaccag cgcgtgccgc
     2701 gcaccagggt ctcgcgcgcc tcgccgagca tctcgatctg cagcgcggcc ggcacctccc
     2761 tgggcaggtc ctcgatcgcc gcccagagcg gcaggagcgc gaaggcgtcc cgggtgatga
     2821 cgtagccgag gcagatatcc gcgaggctgg cgccggtctc gtcctcgagc tcggtgcaga
     2881 agacgtcgag gccgcgattg acgaggctgt tggcgagcca ggtggcgatg atctcgcgcc
     2941 tgaggcggtg ctcggcgatc ggcccggcga agccgcgccg cagcggccgc gggaagtact
     3001 tggcgaggtc gccggcgaaa tagggccggt ccggcagctc ggtcgccagg agatcggtga
     3061 acagcgtcat cttggcgtac gccaggagca ccgccgattc cggccgggtc aggccgcgcc
     3121 cggcccggcg ccgctcggcc agctcggcgt cgtccggcag gaactcgatc gcccggtcga
     3181 gccggccggc ccgctccagc ttgcgcatca gccgggcctg cggctcggcg aagtcggcgg
     3241 cgcgcgcctc ggtcaggctc agggccaggt tctgcagcgt gttgtcgcgc aggcagagtg
     3301 ccgccacctc gtcggtcatc cgggcgagca gcgcatcgcg ctgcttcatg gtcagatcgc
     3361 cggcggccac gacctcgccc agcaggatct tgatgttgac ctcgtggtcg gagcagtcga
     3421 cgccggcgga gttgtcgatg aagtccgtgt tgagccgcac cccgctcgag gctgcctcga
     3481 tgcggccctt ctgggtcatg ccgagattgc cgccctcgac cagcaccttg cagcgcagct
     3541 cccggccgtc gatccgcagg ccgtcattgg cgcggtcctg ggcgtcgtgg tgcgactcgc
     3601 tgctcgcctt gacataggtg ccgatgccgc cgttccacca gaggtcgacc ggggcctcga
     3661 ggatggcgct gatcagctcg gtcggtgccc aggtgtcgcg gtcgaggccg agcgccgcct
     3721 ggacctgtgg cgacaccgtg accgacttcg cgcggcgcga gacgatctcg ccgccggcgc
     3781 tcaagaggct ccggtcgtag tcgtcccagg acgagcgcgg cagcgcgaac aagcgccggc
     3841 gctcggcgaa ggagctcgcc gggtcggggt ccgggtcgag gaagatgtgg cggtggtcga
     3901 aggcggcgag caggcggatc cggtccgaga gcagcatgcc gttgccgaac acgtcgcccg
     3961 acatgtcgcc gatgccgatc accgtgaaga ggtcgacggc cgggtcgagc cccatctcgc
     4021 gaaagtggcg cttgaccgat tcccaggcgc ccctcgcggt gatgcccatg cccttgtggt
     4081 cgtagccggc cgagccgccg gaggcgaagg cgtcgtcgag ccagaagccg taatcgcggc
     4141 tgaccgcgtt ggcgacgtcg gagaagctgg ccgtgccctt gtcggcggcg acgacgagat
     4201 aggggtcgtc gtcgtcgtag cggacgaccc gcggcggcgg caccacggca cccttcacct
     4261 ggttgtcggt gatgtcgagc aggccggaaa ggaagagctt gtagcagcgg atcgcctcgt
     4321 cgacgatcac ctgccgctcg gtcgtctcgg gcggccgctt gacgtagaac ccgcccttgg
     4381 cgccgaccgg cacgatgacg ccgttcttga ccatctgcgc cttcatcagg cccagcacct
     4441 cggtgcggaa atcctcgcgc cggtcggacc agcgcagccc cccgcgcgcc accttgccgc
     4501 cgcgcagatg cacgccctcg acggccgggc cgtagacgaa gatctcgaag gcgggccggg
     4561 gcttgggcat ctccggcacc cgcgccgggt cgagcttgag cgagagatag gccttgggcc
     4621 cgccctcggc ggtccgctgg aaggcgttgg tccgggtggt ggcgtggatg agacccagat
     4681 agcggcgcag gatccggtcc tcgtcgagga tcgccacggc ctccagcccg gtcttgatgc
     4741 gcgcctcgat ctcgaccgtc cgggccgcgc gatcgccggc gaaatccggg tcgaagcgca
     4801 cgttgaagag ggcgacgagg tccgcggcca gagccgggtt cgtcgtgcag gtgttctcga
     4861 tataggcctg gctgaagggc gtgccgagct gctgcatgta cttgcagtag gcgcgcagca
     4921 cgacgacggc ggcggcatcc aggccggcgg cgaggatcag gcgattgaag ccgtcgttct
     4981 cgacggcacc cgaccagatc cgctcgaacg cctcctggaa ggcgtcgcgc acggcgtcga
     5041 catcgacgac ggcgccgctg accggccgca tgccgaaatc gtgcatcgag aagagcttgc
     5101 ccctggcgtt gcgcaccggg tgcgcctcct cgtgcaacac cttgaggccc atgttctcga
     5161 ggatcggcag cgcatcggag agatgcagcg gcagctcgcg gcggaccagc ttgaagcgga
     5221 ccatctcggg gctgtcctcg agccgccggt agagggtcat gccgaggcgg tcgccggccg
     5281 cctgctcgag ccggtccatc acgagaatgt ccggcacggc ggcgcgcgcg gcattgcgct
     5341 cctggtagct cgccgggaag gcgcggctga aggtgcggta gagccggttg ccctcctcct
     5401 cgccctcggc ctcgatcagg gccgcatgca ggcggtcggt ccagctctgc gagagctcct
     5461 ggatctggcg ctccagggcc tcgacgtcga gatcgtccgg caccccctcg ggcgtgcgga
     5521 cgatcaccat gatgcgggcg aggacggcct ccgtgacctg cgcctggaac tcgctgtcgc
     5581 tgccgtcgaa ggcctcgagc aggaggctct gcagccgctc gcgcagcgtc gtgttgtagc
     5641 gctcgcgcgg gatgtagagg aggcacgaga cgaagcggcc gaaggtgtcc tggcggacga
     5701 acaggcggat ctgctggcgg tcctgcagat agaggatctg cgaggtgatc tcgaagagcg
     5761 tgtcctcgtc ggcctggaac agctcgtcgc gcggataggt ctcgaggatg ttgaccagcg
     5821 ccttgccggc atggccggcg cgcacatggc cggcgcgctc gacgatggcg tcgaacttgc
     5881 ggcggagcag cgggatgaag cgcgggctgc ggttgtaggc gaccgaggtg aagaggccga
     5941 ggaggcgatg ctcgccgatc acccggccgt ccggggcgta gcgcttgacg ccgatgaaat
     6001 cgagatatgt cgcccggtgc accgtgctct tggtgttggc cttggtgatg gcgatggcgg
     6061 gcaagggctc ggtcgcccga aggcgggccg cgagcggcat ctccatgaag ctcttcgagg
     6121 gcgccccgtc gtcgtgcgcg cgcaggatgc cgaggccgct gcccttcacc cggcgcagca
     6181 ggagcgaggt gccctcgcct tccagcgcat aggccgagta gccgagaaag gtgaagtggt
     6241 cgtcggccag ccactcgagg aacgccttga tctcggcgag gtcgtcggcc ggcaaatgct
     6301 gcgccgccgg ctccagctcg gccagcatgt cgcccacccg ctcgcgcatc gaccgccagt
     6361 ccacgaccgt cttgcggacg tcgccgagga tgcggacgag atcgccctcg agcgcttcga
     6421 gcacgtcggc gtcgctctgg cggtcgatct cgatatgcat gaagctctcg aggcgggcgc
     6481 tctccggggc accctcgcgg tcgccgatcc attccagccg gccggccgcg tcgcgctcca
     6541 ccgcgaagat cgggtggatg acgaggtgca tgccgaggcc gtgccggctc agctccatgg
     6601 cgacgctgtc gaccaggaag ggcatgtcgt cgttgacgat ctcgacgacc gtatggctcg
     6661 actgccagcc atgctgggcg atgccgggat tgaagacccg caccttcaag gtcccggccg
     6721 ggcgctgccc ggcctggcgc agatgggcga gcgccgcgcc gtagaggtcc tggacctcac
     6781 gcgacaggac gtcggccggg gccacgtcgc gataataggt gcggacgaag gctgcagcct
     6841 gggcggcttc ctcgcccgtc agcttcgcct cgatctcggc gatcaccgcc aggatcagct
     6901 cctgcttctg ctcctcgccc cgcagcacca tgtccacgcc cccctgttcc ttctggcatc
     6961 aaaccacagc cggatgccaa gtagaagcgc agcggcgtgc atgcgcggcg ccggcgacag
     7021 cggcgggctc agccgggcca gctctgctcg ccgccgtcga ccctgagcac ctggcccgag
     7081 acgaaggcgc cgagcggccc ggcgaagagc tcgaccacgc gggccacctc gtcgacggtg
     7141 gcgatccgcc gcagcgtgcc cgcctcgacc agccggccct ggtcgaccgc ccgggtgccg
     7201 agatagcggc cggtccgcgt gtcgcccggg gccagcgagt tgacggcgat gtcgtgcggg
     7261 cggagctgct gggcgaggca gcgggtgtaa tgcacgacgc cggccttcgc cgtggcatag
     7321 atggccccct gctcgcgccc cttgaaggcg gcgatcgagc tgatggtcag gatccggccc
     7381 cggttgcgcg ggatcatccg ctgcgccacc gcccggcaga ccaggatggt cgagaggagg
     7441 ttgctgtcga ggacggcgcg gacatccgcc tccttgaccc agacggcgtc gttcgggtcg
     7501 ggcttgccgc cggcggcggc gatgtcgccc ccggcattgt ggacgaggat gtcgagcgga
     7561 ccgagccgct cggccaccgt atccaccacc cgctcgacga ccgccccgtc ggtcaggtcg
     7621 ccgagcacgg ccagggtcgg cacgccgaat ccggcggcga tcgcctcggc ggtcgcggtc
     7681 agcgtcgtgc cctcgccata ctcggccggg ccgtgctcgc gccttccatg cacgccgatg
     7741 gcgcagccca gggccgccag ccgctcggcg aaggcccgcc cgagccccct gcccgcaccc
     7801 gtcaccagcg ccacctgtcc ctcgagcagc cgctcggcca tcccgcccgt cctccgttcg
     7861 gtgaaatcgt ccgcactgcc gctatggcag ccccgagcct gtcgcaccag ccccggattc
     7921 ggtgagccgt cagggtgatt tgatttgtgc accggccggt gcgggcgcag acttcgacaa
     7981 agctcgttca cgatccggga ggacaagtca tgaaattcgc cgccgcgcgc accggtctcc
     8041 tcgccctggc gctgctgtcc ggcacgagca cgctggcgtc cgccgatgtc gtgctgaggc
     8101 tcggcaacga cacggacccc gccaccctcg accagcacaa gacctcgacc gtggccgaat
     8161 cgcgcctgct gcgcgacctt tacgaagggc tggtcgccga ggacgccgca ggcaagatca
     8221 ttcccggcag tgctctgagc tgggagatct ccgacgacgg gctgacctac acctttcaca
     8281 tgcgcgagga tgccaggtgg tcgaacggcg acccggtgac cgcggacgac ttcctgttca
     8341 cgttccgccg catcatgaat ccagccaccg ccgcgggcta cgccagcgtg ctctttccga
     8401 tcctgaacgc cgaagccgtc gccaagggcg agcggcaggc cggggatctc ggcgtcgcgg
     8461 cacccgaccc gcaaacgctg gtgatcacgc tgaacgcgcc cacaccctac ttcctccagc
     8521 tcctcaccca ccagaccggc ctgccgctgc atcccgcgac ggtcgagagc ttcggcgacg
     8581 cgttcaccaa gcccggcaac ctcgtgtcca acggcgcctt cgtgctcgag agcttcgtgc
     8641 cgaacgacaa gatcgtcatg aggaagaacc cgcaattcca cgacgccgcc aacgtgcagg
     8701 tcgacgaggt ccagtggatc ccgttcgagg accgctcggc ctgcctcagg cgcttcgagg
     8761 ccggtgaggt gcacacctgc tccgacgtgc ccgccgagca gatgaagtac atgaaggaga
     8821 acctgcccga ggcgctcagg atcgccccct atctcggcac ctactacctg ccggtgaagg
     8881 tcgccaagcc cgagttcgcc gacccgcgcg tgcgccaggc gatctcgatg ctgatcgacc
     8941 gcgacttcat cgccgacgag gtctggcagg gcacgatgct ccccgcctat gcgctggtgc
     9001 cgcccgggat cgacaactat gtcgagaacc cgcccgagct cgactacgcc aaggccgacc
     9061 tgctcgaccg cgaggacgcg gcgatagcgc tcctgcagga ggccggggtc gaccccgcga
     9121 ccctcgacat ccagctcagc tacaactcgt ccgagaacca ccgcaacacc atggcggcga
     9181 tctcggacat gctctccaac gtcggcatcg agtcgtcgct caacgagatg gagggcaccg
     9241 gctacttcaa ctacctgcgc gagggcggcg acttcgacat cgtgcgcgcc ggctggatcg
     9301 gcgactattc cgacccgcag aacttcctct atctcaacga gaccggcgtc tcgttcaact
     9361 attccaagtg gtcgaacgag gcgtacgatg ccaagatgcg cgccgcgtcg caagagaccg
     9421 atctcgacga gcgcgcgaag ctcctggccg aggccgaggc gatctatctc gccgaggtcc
     9481 cgacgatccc catcctgttc tactcctcca gggcgctcgt ctccgaccag atctccggct
     9541 acgaggacaa cctgctcgac catcacgcga cccgctggct cagcctgaac cagtgagcgc
     9601 ccgtccggcg ggcccgtgcg cgggcccgcc gctcatcgag ccggcggtgg cgcgatgctg
     9661 acctacaccc tgcgccgact ggcgagcgcc gtcccgacgg tgctcgtcat catcacgctg
     9721 acgttcttca tgatccgcct cgcacccggc gggccgttcg atctcgagcg gccgctcgac
     9781 ccgctgatca tggccaacct cgagaaggcc tacaatctcg atgcgccgct ctggtcgcag
     9841 tacctgacct atctcggcaa cctgctgcac ggcgacctcg gcccgagctt cacccgccgc
     9901 gacttctcgg tgaacgagct cttcgccgtc ggcatgccgg tctcgatcct gctcggcggc
     9961 ctcgcgctcg tcaccgccgt cgtcctcggc accctgctcg gcgcatgggc ggcgctccgc
    10021 cagaacaccg gcgtcgacta cgccgtggtc gggctcgcca ccttcggcat caccacgccc
    10081 accttcgtgg tggcgccgct cctgagcctc accttcggcg tcgtgctggg catcctgccc
    10141 gcgggcggct ggtcgaacgc caacccggcc tactgggtcc tgcccatcgt caccctggcg
    10201 ctgccgcagg tggcggtgat cgcgcggctg gtgcgcggcg ccaccatcga ggcgctgcgc
    10261 tcgaaccacg tgcgcaccgc gcgcgcctac ggcctgccga cccgcatcgt cgtcggcgtc
    10321 cacgcgctgc gcgcggcgat gctgccggcg gtcagctatc tggggccgac cgcggccggg
    10381 ctcctgaccg gctcggtggt ggtcgagacg atcttcggta tccccggcat cggccgctac
    10441 ttcgtccagg gcgctttggg ccgcgactac acgctggtga tgggcatgat cgtggtgatc
    10501 gcgctcttca tcatcgtctt caacctcctg gtcgacctcg cctacgcctg gctcgatccg
    10561 cgcgtccggt tcgactgagc ccatggcggt ccagcccgac ctcatcgagg acatcccggc
    10621 ggtcgcgggc cgctcgctct ggcaggacgc gtgggcgcgg ctgcggcgca accgggccgc
    10681 cgtggcgagc atgatcgtcc tggcggcgct gtgcctggtc ggcatcttcg ggccgatggt
    10741 ctggccgcac ccctacgacc aggtctaccc gaagttcgtg cgcgtgcccg cgagcctcga
    10801 ggcctatccc ggcaccgaca gcatcctgcc gggcttcgag ggcctcctgc aacgcgcccg
    10861 gctcgagcac ggcgagccgg tgctcgaggg cggcgaggtc agcgtcgcgg tggccgccga
    10921 cgagccgatc gacccgcgca tcctgcgcta cttcgagcgc tccgatctct tcagcaatcc
    10981 tcgcctcgag ctcgccgacg acgggctttc cggcaccctc gccgtcgacg tcgagcggct
    11041 gtatttctac ttcggcaccg acgccatcgg ccgcgatctg gcggcacgca tcatgatcgg
    11101 cgtgcggatc tcgctcgcca tcggcatcct cgcctcgatc atggcgctcg tgctcggcgt
    11161 cacctacggc gcggtctcgg gctatctcgg cggccgcgtc gacaacgtga tgatgcgcgt
    11221 cgtcgacatt ctctactcgc tgccgttcat cttcttcgtg atcctgatgg tggtgttctt
    11281 cggccgctcg ctgatcatca tcttcatcgc catcggcgcc accgaatggc tcgacatggc
    11341 ccgcatcgtg cgcggccaga ccctcagcct caagcggcag gagttcgtcc aggccgccga
    11401 ggcgctcggc gccaccaaaa ccggcatcct catgcgccac atcatcccca acacgctcgg
    11461 gcccgtcgtg gtgttcgtca ccctcctggt acccaaggcg atcctgctcg aatcgctcct
    11521 ctccttcctc ggcctcggcg tgcaggaccc cttgacctcg ctcggcctcc tgatctccga
    11581 gggtgccgcc aacatgcgcg gtgccctctg gctgctgatc tggccggccc tgaccctgac
    11641 gctgatcctc ttcgcgctca acttcctcgg cgacggcctg cgcgacagcc tcgacccgaa
    11701 ggatcgctga gatggagccc gtcctctctg tccgcgacct ccacgtcgag ttcgagacca
    11761 acgacggcat ggtgcaggcg gtcaggggca tcgacctcga cgtcatgccc aacgaaagcg
    11821 tcgccatcgt cggcgaatcc ggctcgggca agagccagac caccatggcc atcatggggc
    11881 tgctcgcctc gaacggccgt gcgatgggct cggccaggta ccgcggcacc gagatgctcg
    11941 gcttgcgcga gagcgagatc aacaggatcc gcggcgacaa gatcaccatg atcttccagg
    12001 agccgatgac ctcgctcgat ccgctctacc gcatcggcgc ccagctcgcc gagccgctgc
    12061 gcttccaccg cggcctcacc cggtcccagg cccgcccgcg catcatcgag ctgcttcgcc
    12121 tggtcggcat ccccgagccc gagcgccgca tcgacgccta cccgcacgag ctctccggcg
    12181 gccagcgcca gcgcgtcatg atcgccatgg cgctcgcctg cgaccccgac gtgctgatcg
    12241 ccgacgagcc gaccaccgcc ctcgacgtca ccgtgcaggc gcagatcctc gagctgctcg
    12301 ccgggctgca ggccaggctc ggcatggcga tcgtcttcat cacccacgac ctcggcatcg
    12361 tccagcgctt cgccgaccgg gtctacgtga tgcgcgccgg cgaggtggtc gagaccggcg
    12421 agaccgcccg gctcttcgcc cggccatcgc atccctacac gaagatgctg ctcgccgccg
    12481 agcccgaggg caccaaggcg ccgccgccgg ccgaggcacc cgacctgatg accggccagg
    12541 acgtgcgcgt cgtcttccgc tcgggcggcg gctggttctc gaaggcccac gagctgcgcg
    12601 ccgtcgacgg tgtcagcgtg cggctgcgcc agggccagac catcggcatc gtcggcgagt
    12661 cgggctccgg caagtcgacg ctcggccgcg ccctgctgcg cctcctgccg agccagggca
    12721 ggatcgagta tctcggccgt ccgctcgacc ccgaccccgc caccatgcgg ccgatgcgcc
    12781 gcgagctgca gctcgtcttc caggacccct tcggctcgct cagcccgcgc atgaccgtcg
    12841 gccggatcat caccgagggc ctgctcatcc acgagcccgg cctcgcggcg cgcgagcgcg
    12901 accggcgcgc cggcgaggcg ctgaccgagg tcgggctcga ccccgccatg cgcaaccgct
    12961 acccgcacga gttctccggc ggccagcgcc agcgcatcgc catcgcccgg gccatgatcc
    13021 tcaagccccg ggtgatcgtc ctcgacgagc cgaccagcgc cctcgaccgc tcggtgcaga
    13081 agcagatcgt cgacctcctg cgcgacctgc aggcggcgca cgggctctcc tacctcttca
    13141 tcagccacga cctcgcggtg gtgcgcgcgg tggcggacta catcatcgtg atgaagggtg
    13201 gccggatcgt cgaggagggc ccgacggcgg cgatcttcga ccatccggag atggactaca
    13261 cccgcacgct gatggccgcg gcgctcgact cgagccggtt ccgcgagtcc gccgtcgacg
    13321 gctgatgccg aaagagccct gccgccagcg gtctcagagc ccctcggcgg cctcgcgcac
    13381 catgcggtac tggacccgca tcagggacag gcggcggtag aagttgagca ggtcgcgggc
    13441 cgggtcggtc atgcgctggt agcccgaggc gaaggcgacg atggtgccga ccgtggtcga
    13501 gccttcgagc accagccagc cgccgatggc gaggatgctg agcggcccca gatggccgag
    13561 caggttgttg aacgacttga gcgcgaactt gacgacggcg aagcggatcc tgaggccgta
    13621 gatggtctcg accagccgat cgagcctggt ctcggcggcc tcgctccggt cgtcgtcgag
    13681 cgccgcgtcg ccgagctcgc gcgtggcctc ggtcttgcgc ccggcgagcc ggtcgagccg
    13741 cggctgcatc agagccacgg cgacgatcga gggcacgaag aggccgagcg cgacgatggc
    13801 gaccgccggg tcgacggcca gcatgtagcc ggcgacgctg acgacgatgc cggcctgcag
    13861 cagcgggaag gcgacggcct cgccgacgaa gccgccgagc ttctccgcct cggcgacgag
    13921 gatcgactgg gccgtgccgt cggcgagccc gtcgccatcc tcgatgatgc gccggcgcag
    13981 ccgccgggtg atgccctgcg ccacgcgctc ctggtagatg ttgcgaacca gcttcagccc
    14041 ggcctggagc agcacggcag cgaggtagag gccggcgagg atcagcagca gccgggggtt
    14101 gcggccgccg atggcatcgt cgatcagccg tcgctgcagg tcgatcggcg ccagggtcag
    14161 ggggaagatg gcgagcgtca gcagcacgag cggcacctgc tggcgccggc tcgcggccat
    14221 cacataggca tagaggccgc gcggcagctc cgccagcggc cggccgccga cggccggcag
    14281 caaggcccgc aagggcgccg ttcccgaccc gcccgcggtc acctgtcgct ttcgccgtcg
    14341 agcaccatca gccgaccgat agacggtcgg cgcccttcac gccagcggca cgccaaagcc
    14401 ggttcggcac ctgccggccc ggctcagccc ccgctgccca cgctgaagct gggaatggtc
    14461 ggcatcgtgg gcatggtggg catggccggc atggccgccc ggctggagaa gccgtcgagg
    14521 gccgacaagg gcgtcaacga caccgtcgtg gtcggggcgg tcaccggcgg gccggcgggc
    14581 gcggccgtgg gcgccgtcgt cggggcaaag gtgaaggtcc ggcccgtcaa cccgccggtg
    14641 gcgaacccgc cggtcctggt cgtgacgacg ggcgccccgt tgccgtcgga gctgctggtg
    14701 atcgtctgcg aggcgaaagc gccggccgtg ccggggcccg tgccgctgat gaccgcgatc
    14761 gtgccgccgc cgccactgga cgtgaccgtg cggctgtcct ccagggtggc aaccgtcgtg
    14821 ccgccggccg gcttcggctg ggcggtggcg gccgccccct cgccgacgag cgtctccagc
    14881 tcatgcctgg cggcctcgac agtcgcatgg ccgtcgacca cgtcgccgcc gctcggccgt
    14941 gcctcctgga gggcacggac cgcagccgtg ctcgcggtga tccagtcgag ctgcgccgcg
    15001 tcgagacggg tcggctcggc agcgcccgcg acattggtga aggtgaacgc ggctgctgcg
    15061 agcaggatga tgcgcatgat cgtttccctt cccccctctc ggtcgaaggc tacaattgaa
    15121 gcccgaacgc tctcgctgac aaggtgaaaa acgactaacc atgccagggc tgcacccggc
    15181 tattgtcgcc tggcagagca ggtgaccggc agcccaatca gcgatggaga cccgaccttg
    15241 agcccggacg tgctgatcgc cccctcgatc ctctccgccg acttcgcccg gctcggcgcc
    15301 gaggtggcgg cggtcgaccg cgccggcgcg gactggatcc atctcgacgt catggacggg
    15361 cactacgtcc ccaacatcac cttcggcccc gccatcgtcc gggcgatccg caagagctcg
    15421 gcgaagctct tcgactgcca tttgatgatc gagccggccg accccttcat cgccgccttc
    15481 gccgaggcgg gcgccgatct cgtcaccgtc cacgccgagg ccaccaccca cctcgaccgc
    15541 tcgctgcagc tcatcaagag cctcggcaag caggccggcg tcgccctcaa ccccgccacc
    15601 ccggccacgg ccatcgagca cgtgctcgac cggctcgacc tcgtcctggt gatgaccgtc
    15661 aaccccggct tcggcggcca ggccttcatc ccggccatgc tcgagaagat ccgaagggtc
    15721 agggcgctca ccgccggccg cggcatccgc atcgaggtcg atggcggcat caacgccgag
    15781 acggcgccag aggtcatcgc cgccggcgcc gacgtgctgg tggcgggctc cgcgatcttc
    15841 ggccagccgg accacgcggc cgcgatcgcc gcgctcaggc ccaccgccgt cgcctgaccc
    15901 ttggtcagcc gcagaagggc aggctctgca gcatcaggat gaggctcgcg tcgccgtagc
    15961 cccgccaggc gagcgccagg gcgacgacgc cggcgaggcc gaggagccag aggccggccc
    16021 ggcgggcgcg ctccgtcatg cgccggctcg cgcggcctcg gctgtcaccg ggcgctcgcg
    16081 gatcggcagg ttgagcaagg ccgcgatgag gctcagggcg atcgccatgc cccaggcgag
    16141 gtcgtaggag ccgagccggt cataggcgac accgcccagc cagccgccga ggaaggcgcc
    16201 gacctggtgg ctgaggaaga ccacgccacc cagcatcgac atgttgcgcg ccccgaacat
    16261 ggcgacgacg acgccgttgg tgagcggcac ggtcgagagc cagaaaaggc ccatgagcac
    16321 gccgaagagg atggccgagc ccgcggtgat cggcaggtag acgaagagcg ctatggcggc
    16381 ggcgcggccg agatagaccc aggcgagcag gccgggcttg gcgaagcggc cgccgagcca
    16441 gccggccgcc agcgtgccca ccacgttgac gagcccgatg agcgccagca cggtggcggc
    16501 gacggaagcg tcgatgcccc gatcgccgag gaaggccggc aggtgggtgg cgatgaaggt
    16561 cacctggaac ccgcagacgc cgaagccgag ggccagcagc cagaagccgc gatgcgccat
    16621 ggcggtccgg aaggcggcgc cggccgaggg gccgggcgcc tggccggcat tgccgcggtc
    16681 ggcgccgagc atcgcggcga gcggcagcat gagggcggaa agcgccgcga gggccagcag
    16741 ggccgcggcc cagcccaggc cgtcgagcag gcccagcgcc aggggcagca tcacgaactg
    16801 gccggccgag ccgaccgcca tggtgaggcc gaaggccatg ctgcgccgct ccggcggcag
    16861 cgcccggctg accgcgccga agacgatcgg gaaggtcgtg ccggcgagcc cgagcccgac
    16921 caggagcccg gcggcgacga ggaaggccgg cgcacccaag ggctgcgcca tgagcgccac
    16981 gcccagcacg tagaggaccg caccggtgag gatcacccgc cgcgccccga gccggtcggc
    17041 ggccatgccg gcaaagggct gcgcgaggcc ccagacgagg ttctgcgccg ccatggcgaa
    17101 gccgaacacc tccctgcccc agccattggc catcgagacc ggctgcagga agaggccgaa
    17161 ggtgaaccgg atgccgagcg acagggcgac gatgacagca ccgcagacga ggagcgccgc
    17221 gaccgagtcc ctggcgctgc cccgggtcat gaccggagcc ctcccgaagc cggcgcccgc
    17281 aggcgtcgca gcgcgcggcc gaacgcctcg agctcggaga tggtccgctg cccgagcccc
    17341 gagggtctca gcgccgcgtc gtgattcccg gtcggcgacc gggcggcgcg gcgggcattg
    17401 gtgcaatggc gggcgttctc gggcatggcg ccggtataga cccgcatcct gcccggcctg
    17461 caagagcgac ggcggcgccc cgcggccccg gattggctcc tagaacctgt accccaggtt
    17521 gagcgcgaag aagagcgccc ggttctcctt gtagctgccg ccgaagaggt cggcgtcgat
    17581 gtcggcgtcg ccgagatcgg cgtagaccag cgagccgccg acggtgacgc gttccgagac
    17641 cgcgtactgc gcccccaccg agtagcggta ctggcggtcg accggcaggt ccgcgcgccg
    17701 gttcctgtcg gagaccgggc tcgtgtcgta gccgaatccg gcctggagca ggaggtcgtc
    17761 cacgggcctg tagtgcacgc cgagcgagaa cccgtatgta tcgcgccagt tggcgggaat
    17821 cgcggtcgag ccgcgatcgg ccgagaccgg aatgtcgccg aaggcgctcc agtcctccca
    17881 gcgcaccgag ccgagcagcg ccacgctgtc gctcaggtcg tgatagacgc tcgccttgac
    17941 gtattgcggg aaggtgaagt cgagcccggc gctggcctgc tgcccgagcg ggcggacatc
    18001 gacgtcgccg gagaggttca gcttcgcctc cgagttgtag atcacgccga atcgcgtgtc
    18061 gtcggtcagc tcgaaaaggg cgctgaggtt gaaggtgacg tcggtgtcgt cggcatcctc
    18121 gaccctgacc cgcgcattgc cgccgaccgc cgatggccgg atcgtctcgt tcaggttggc
    18181 ataccagagc gtcgccccga cgccgagcga cagccggtcc gtcaccgcgt aggcgaccga
    18241 cggggtgatc ccggcgacga ccagcgacac gtcggtcacc tggaaccgcc cgacccagtc
    18301 gtcgtcgtac tccagtgctg caccagtgat gccgccggcc gagaggccca ccgcccacct
    18361 ctcgtcgagc tcgagaacgc cggcgagggt ggcgagcggc gccagggttc ccgcatcgcc
    18421 gccgtcattg ccctggatgg ccgaccgatc ggggtcgaac ttgacctcgg tcagcccgaa
    18481 ccccgccccg accatgagcg cactgccctc gagccgggtc atgccggccg ggttgtgcag
    18541 ggccgtcgac gcattgtcgg cccaggcctg ggcgccggcc gaagccgtgc ccatgcttgg
    18601 cgtcgcgaac tccgagatgt agagcccgcc ggcctcggcg ggcccaccgg ccgcgaggcc
    18661 gacgaggaag gccccgctga cccaggccca cgacgtccgc tgcaacatcg gcaaatccca
    18721 tcatgcacgt ctcgacgtcc atggaccgct cgggcccggc ggccatcggc catcgccatc
    18781 aaactcgacg gtcccgtcga tttgacaagg gaaaaagcct ggccgaccct tgggcgaacc
    18841 ccacggcgcg gtgtcgcggc ggcccttcgc gatgacgatg gcccgtccgg ctcgtgtcca
    18901 cgatccacca gaccgtcggg agatctccca tgtcactggc tcttctgcgc cgttcgctcc
    18961 tggcctcggc cctggccggc ctcctcgtcg ccccgctccc cgcagcactc gtggccccga
    19021 ccgccacggc ccaggtgccg cccgacgtgc tggtcgtggg ccagatcgcc gagcccgcgt
    19081 cgatggaccc gcatgtggtc accgcggtca acgacttccg catcctggtc aatgtctatg
    19141 atggcctggt ccgctatcgc cccggcacgc tggaggtcga gccggcgctc gccgagagct
    19201 gggagatcag cgaggacggc acgacctaca ccttcaagct ccgcgacggc gtcaccttcc
    19261 acgacggcac gcccttcgac gccgcggcgg tgaagttcaa cttcgaccgc atgctcgacg
    19321 agagccatcc gcagcatggc accggccctt tcccgctctc cttcttcttc agcgccatcc
    19381 aggagaccga ggtggtcgac ccgctgaccg tgcggttcca cctgaacgag ccctacgcgc
    19441 ccttcctctc gaacctcgcc tatcccaccg gcctcatcgt ctcgccgacc gcggtcgaga
    19501 cgcacggacc cgactacgcc cgcaaccccg ccggcaccgg gcccttcgcg ttcagggtct
    19561 gggagtccaa ccggcaggtg gcgctcgagc gcaacgagga ctactgggac ggcgccccac
    19621 ccctgcgagg cgtggtcttc cgcccgatca ccgatgccaa tgcgcgcgtc tccgagatgc
    19681 tctcggg
//
LOCUS       DAOTFI010000072        19545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3353_length_19545_cov_7.738276, whole genome shotgun sequence.
ACCESSION   DAOTFI010000072 DAOTFI010000000
VERSION     DAOTFI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19545
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3353_length_19545_cov_7.738276"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..382
                     /gene="rpsJ"
                     /locus_tag="P5558_09175"
     CDS             74..382
                     /gene="rpsJ"
                     /locus_tag="P5558_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRY24520.1"
                     /translation="METQNIRIRLKAFDHRVLDQSVREIVGTAKRTGARVRGPIPLPN
                     RIERFTVNRGPHVDKKSREQFEIRTHKRLLDIVDPTPQTVDALMKLDLASGVDVEIKL
                     "
     gene            393..1112
                     /gene="rplC"
                     /locus_tag="P5558_09180"
     CDS             393..1112
                     /gene="rplC"
                     /locus_tag="P5558_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRY24521.1"
                     /translation="MRTGMIARKMGMTRLFGVDGSHIPVTVLKVDDCEVVAVRTVEKD
                     GYTAVQLGAGKAKPKNVTKPMRGHFAKAKVEPKAEVAEFRVAEDALLGVGERVHVGHF
                     VAGQFVDVAGLSIGKGFAGAMKRHNFSGLRASHGVSVSHRSHGSTGQRQDPGRVFKGK
                     KMAGHMGHRNVTVQNLEVFGVDPDQGLILIRGGVPGAKGSWLRVTDAVKKALPKDAPF
                     PAAVVRAGEAMEAIVAEEGGE"
     gene            1115..1735
                     /gene="rplD"
                     /locus_tag="P5558_09185"
     CDS             1115..1735
                     /gene="rplD"
                     /locus_tag="P5558_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRY24522.1"
                     /translation="MQVDVKNLAGEVVGSTELNDAVFAVPVRRDILHRVVTWQLAKRR
                     AGTHKIKTRGEVSGSTAKIYKQKGTGRARHGSKRANIFRTGGRAFGPTPRDHSTDLTK
                     KLRKLGLRCALSAKAGAGQLIVLDEAALTEPKTRLMGGVLDKLGLGSALFVVAGEPER
                     NFALASRNLPTIDLLPQEGANVYDILRRDVLVLTRDAAKLLEERLA"
     gene            1732..2025
                     /locus_tag="P5558_09190"
     CDS             1732..2025
                     /locus_tag="P5558_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRY24523.1"
                     /translation="MTTARNYDVIVAPVITEKATRVSENGQVVFKVRLDADKGEIKEA
                     VESIFGVKVTAVNTLVQKGKTKRFRGRPGKRNDIKKAFVTLADGQAIDVTTGI"
     gene            2036..2878
                     /gene="rplB"
                     /locus_tag="P5558_09195"
     CDS             2036..2878
                     /gene="rplB"
                     /locus_tag="P5558_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019297986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRY24524.1"
                     /translation="MALKQYNPTSPSRRQLVTVDRSALWKGKPVKKLTEGLTKSGGRN
                     NHGRTTARYIGGGHKRAYRMVDFKRRKFGVNGTVERLEYDPNRTAFIALIGYEDGEQA
                     YILAPQRLQAGDKVIAGERVDVKPGNAAPVRAIPIGTVIHNVELKPGKGGQIARSAGS
                     YAQIVGRDGDYTQVRLGSGEIRRVHSTCMASIGAVSNPDHQNINYGKAGRKRWLGKRP
                     HVRGVAMNPVDHPHGGGEGRTSGGRHPVTPWGKDTKGTRTRSNKKTQGMIIRSRHKAK
                     KRGG"
     gene            2881..3159
                     /gene="rpsS"
                     /locus_tag="P5558_09200"
     CDS             2881..3159
                     /gene="rpsS"
                     /locus_tag="P5558_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRY24525.1"
                     /translation="MPRSVWKGPFVDGYLFDKVEKARSSGRNEIIKTWSRRSTIMPDF
                     VGLTFAVYNGKKFVPVYVTENMVGHKLGEFSPTRTYTGHGADKKARRK"
     gene            3162..3551
                     /gene="rplV"
                     /locus_tag="P5558_09205"
     CDS             3162..3551
                     /gene="rplV"
                     /locus_tag="P5558_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRY24526.1"
                     /translation="MGKPKQERRLADNEAMAVATLVRSSPQKLGLLAGMINGMKVDKA
                     LVALEFNRKRVSEVVFKALQSAVANAENNHALDVDRLYVAEASVGKRLVMKRFKPRAR
                     GRAGRVVKHFSQLRIVVQERAEQEEAA"
     gene            3551..4231
                     /gene="rpsC"
                     /locus_tag="P5558_09210"
     CDS             3551..4231
                     /gene="rpsC"
                     /locus_tag="P5558_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRY24527.1"
                     /translation="MGQKVNPVGLRVGINRTWDSRWYADRDYAQLLADDVEIRRFLMK
                     RLAQAGVSRIVIERPAKKARVTIYSARPGVVIGKKGQEIENLRRDLAKLCGGEVHLNI
                     VEIRKPELDARLVAENIAQQLARRVTFRRAMKRAVQSAMRLGAQGIRVTCGGRLGGAE
                     IARTEWYREGRVPLHTLRANIDFGKATSHTPYGTCGVKVWVFRGEIMEHDPMAFDQRF
                     NEQNPGRA"
     gene            4250..4672
                     /gene="rplP"
                     /locus_tag="P5558_09215"
     CDS             4250..4672
                     /gene="rplP"
                     /locus_tag="P5558_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRY24528.1"
                     /translation="MLQPKRTKFRKAHKGRVRGMTKGGAELNFGAFGMKATEGGRITA
                     RQIEAARRAITRHLKRAGRVWIRIFPAKPVSKKPAEVRMGKGKGSVEFWVAPVQRGRI
                     MFEIDGVPHQLAEEALQRASAKLPIGTRFVHRVQEDAA"
     gene            4669..4872
                     /gene="rpmC"
                     /locus_tag="P5558_09220"
     CDS             4669..4872
                     /gene="rpmC"
                     /locus_tag="P5558_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRY24529.1"
                     /translation="MNASDMRAETVDQLSDRLLQLKKEQFNLRFQRATGQLEGTARMS
                     VVRRDIARIKTVLHERRAAGGAG"
     gene            4882..5145
                     /gene="rpsQ"
                     /locus_tag="P5558_09225"
     CDS             4882..5145
                     /gene="rpsQ"
                     /locus_tag="P5558_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRY24530.1"
                     /translation="MPKRVLQGVVVSDKGEKTVIVKVERRFMHPLYKKYIRRSKRYAA
                     HDESNAVPIGAKVRIEECRPLSKTKRWLVIGDGSATEAAPASE"
     gene            5155..5523
                     /gene="rplN"
                     /locus_tag="P5558_09230"
     CDS             5155..5523
                     /gene="rplN"
                     /locus_tag="P5558_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRY24531.1"
                     /translation="MIQVETNLEVADNSGARRVQCIRVLGGSHRRWGSVGDIIVVAVK
                     EAIPRGKVKKGDVHHAVIVRTAKEIHRADGSAIRFDKNAAVLINKQGEPIGTRIFGPV
                     TRELRAKRFMKIVSLAPEVL"
     gene            5520..5840
                     /gene="rplX"
                     /locus_tag="P5558_09235"
     CDS             5520..5840
                     /gene="rplX"
                     /locus_tag="P5558_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRY24532.1"
                     /translation="MMAARIRKGDRVVVIAGKSKGVEGEVLKVLPAENRVVVQGAQQV
                     KKHTKPSARGAGGIESREAPIQVSNVAHIDPTDGKPTRIGFKFLDDGRKVRFAKRSGE
                     IIDR"
     gene            5851..6393
                     /gene="rplE"
                     /locus_tag="P5558_09240"
     CDS             5851..6393
                     /gene="rplE"
                     /locus_tag="P5558_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRY24533.1"
                     /translation="MTARFKQRYDEVVRGQLQEQYGYKNVMQVPRLEKIVINMAVGKA
                     VTDSKKMTAAVGDLTKISGQKPAVCRAKKSVANFKLREGMPIGTKVTLRSQRMYEFLD
                     RLVTIALPRVRDFRGISPKSFDGRGNYAFGLKEQIVFPEINYDEVDEIRGMDIIICTT
                     ARTDAEALSLLKGFDMPFRT"
     gene            6410..6715
                     /gene="rpsN"
                     /locus_tag="P5558_09245"
     CDS             6410..6715
                     /gene="rpsN"
                     /locus_tag="P5558_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRY24534.1"
                     /translation="MAKKSAIEKNKRRREMVAQQAAKRAALKAVIANREGDPEVRFEA
                     VLKLAALPRNGARVRIRNRCTLTGRPRAYYRKFGMSRIALRDLASEGQVPGVVKSSW"
     gene            6731..7129
                     /gene="rpsH"
                     /locus_tag="P5558_09250"
     CDS             6731..7129
                     /gene="rpsH"
                     /locus_tag="P5558_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRY24535.1"
                     /translation="MAMSDPLGDMLTRIRNGQMAQKASVRSPASNLRRNVLEVLQREG
                     YIRGYVADEVIDGKSEISIELKYHNGEPVIRELRRVSKPGRRIYAGVRELPEIYNGLG
                     IAILSTPRGVLSDSEAREAKVGGEVLCTVF"
     gene            7132..7665
                     /gene="rplF"
                     /locus_tag="P5558_09255"
     CDS             7132..7665
                     /gene="rplF"
                     /locus_tag="P5558_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRY24536.1"
                     /translation="MSRIGKHPVAIPGGVDIAVDGQTVKVKGKLGSLEETLPGEVVVA
                     LEDGSLAVRPRDESKRAAQMWGLARTLVQNMVTGVSEGFTRRLDISGVGYRAAVDGNI
                     LNLQLGFSHDIKFAVPPEVKIACETPTAIVISGFERQRVGQIAAEIRSFRPPEPYKGK
                     GVRYSDEVVRRKEGKKK"
     gene            7668..8030
                     /gene="rplR"
                     /locus_tag="P5558_09260"
     CDS             7668..8030
                     /gene="rplR"
                     /locus_tag="P5558_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRY24537.1"
                     /translation="MLKASERFSRRQRRTRFALRQAANGRPRLSVFRSGKHIYGQIID
                     DEAGRTLVAASTLEQPLRGELKTGADMAAAQRVGKLLAEKARAAGVESVVFDRGGYKY
                     HGRVKALAEAAREGGLTF"
     gene            8051..8602
                     /gene="rpsE"
                     /locus_tag="P5558_09265"
     CDS             8051..8602
                     /gene="rpsE"
                     /locus_tag="P5558_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRY24538.1"
                     /translation="MARPQRGEDRQDSELVDRLVNVNRVAKVVKGGRRFGFSALVVVG
                     DGKGRVGYGSGKAREVPEAVRKATEQAKRGMIRVPLRQGRTLHHDIVGHFGAGRVLLR
                     AAPPGTGIIAGGPMRAVLEALGVKDVVSKSLGTSNPHNMVKATFEALQLVNSPRTVAA
                     KRGKNVRDIIGKREPGAEATAEA"
     gene            8607..8813
                     /gene="rpmD"
                     /locus_tag="P5558_09270"
     CDS             8607..8813
                     /gene="rpmD"
                     /locus_tag="P5558_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRY24539.1"
                     /translation="MAKTSKTAKTIKVTQTGSPIGRPADQERTLISLGLNKLWRSREV
                     TDTPANRGRIRKVEHLLRVESDGA"
     gene            8830..9330
                     /gene="rplO"
                     /locus_tag="P5558_09275"
     CDS             8830..9330
                     /gene="rplO"
                     /locus_tag="P5558_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRY24540.1"
                     /translation="MRLNELRDNPGARYKAKRLGRGIGSGKGKTAGKGHKGQKARSGG
                     AVRVFEGGQMPIYRRLPKRGFKNVNRQSFVVVNLGTLQAALDAGKLDAATPIDAGRLV
                     ETGVLRRTLDGVRLLAKGELKAAVTVEVTGASKAAIAAVEKAGGKVVLLGGAPVEAAA
                     DTGSEA"
     gene            9341..10684
                     /gene="secY"
                     /locus_tag="P5558_09280"
     CDS             9341..10684
                     /gene="secY"
                     /locus_tag="P5558_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRY24541.1"
                     /translation="MASVADQLASNINFGAFAKATELKRRIWFAVGCLIVYRIGTYIP
                     LPGIDPQVMAQIFAQQAGGIMGMFNMFAGGALERMSIFALGILPYISASIIMQLMTAI
                     SPSVAQLKKEGEAGRKKINQYTRYLTVLLAAVQAYGLSVGLESMGGPGGSAVIEPGYF
                     FRTSTVITIVGGTMFLMWLGEQITARGIGNGISLIIFTGIIATLPSALASMLELGRTG
                     ALSPLFIIAILIGGLAVIAFIVFMERAQRRIIVQYPKRQQGNKMFGGESSHLPLKLNT
                     SGVIPAIFASSLLLMPATLGSFSGPGAPEWLLVVSALLGRGQPLFILIMVGLIVFFAF
                     FYTSIVFNPQETADNLKKHGGFIPGIRPGQRTAEYLDYVLTRLTTVGALYLAAVCVMP
                     EILISQYSVPFYFGGTSLLIVVAVTMDTVAQIQSHLIAHQYEGLIKKSRLRGRRG"
     gene            10684..11271
                     /locus_tag="P5558_09285"
     CDS             10684..11271
                     /locus_tag="P5558_09285"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRY24542.1"
                     /translation="MNLVLFGPPGAGKGTQAARLRDGHGLAHLSTGDMLRAAVEAGTP
                     IGREAESVMASGRLVSDELICGVVAERIDAPDCANGFILDGFPRTLAQAEALDAMLRG
                     KGKAIDAVIEIRVDEAVLVERITNRIQETGGARADDNVETLKKRLEVYRAQTAPILPF
                     YAEKGILHTVDGMQPMDRVAAEIEAALAPGRASGT"
     gene            11421..11798
                     /gene="rpsM"
                     /locus_tag="P5558_09290"
     CDS             11421..11798
                     /gene="rpsM"
                     /locus_tag="P5558_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRY24543.1"
                     /translation="MARIAGVNVPAQKPVGIALTYIYGVGRTTSEKILQQCEVPFERR
                     VNTLSDAELARIREAIDRDLKVEGDLRREMAMSIKRLMDLGCYRGLRHRKGLPVRGQR
                     THTNARTRKGPAKAIAGKKKVKK"
     gene            11802..12188
                     /gene="rpsK"
                     /locus_tag="P5558_09295"
     CDS             11802..12188
                     /gene="rpsK"
                     /locus_tag="P5558_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRY24544.1"
                     /translation="MAQDTKLRRRERKNISSGIAHVNATFNNTMITIADAQGNAISWS
                     SAGSQGFKGSRKSTPFAAQMAAESAGKKAMEHGMRTIEVLVKGPGSGRESALRALQAV
                     GFTVTNIRDVTPIPHNGCRPRKRRRV"
     gene            12328..13344
                     /locus_tag="P5558_09300"
     CDS             12328..13344
                     /locus_tag="P5558_09300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRY24545.1"
                     /translation="MIQRNWQELIKPQKLNIQPGRRPDREATIIVEPLERGFGITIGN
                     ALRRVLLSSLQGAAVSAIQIDGVLHEFSSIPGVREDVTDIVLNLKSMGVRLHSDVPKR
                     VYLKAEGPKVVTAGMIETTHDVEIMDPDQVICHVDEGGKVSMELTISGGKGYVPAHLN
                     KSEDAPIGLIAIDAIYSPVRKVMYRVDNARVGQQTDYDRLVMQVETDGSVSSDDAVAL
                     AARILQDQLGPFINFDEPHVGQAAERRPELPFNPNLLRKVDELELSVRSANCLKNDNI
                     VYIGDLVQKTEGEMLRTPNFGRKSLNEIKEVLAHMGLHLGMDVAGWPPENVEELARKV
                     EEPF"
     gene            13383..13811
                     /gene="rplQ"
                     /locus_tag="P5558_09305"
     CDS             13383..13811
                     /gene="rplQ"
                     /locus_tag="P5558_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRY24546.1"
                     /translation="MRHGNSGRKLNRTASHRQAMFQNMVQALVKHEQITTTLPKAKEL
                     RPIVERLITLGRGGDLHSRRLLIARLRCETTAGKIMDVLAPRYKERPGGYVRIMKAGF
                     RYGDNAPMAVIELVDRDVDAKGQDSGPVQHDDDEGEAVAA"
     gene            13780..15279
                     /locus_tag="P5558_09310"
     CDS             13780..15279
                     /locus_tag="P5558_09310"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRY24547.1"
                     /translation="MTTRARQSLPDCGRAPLARALLVVLCLVAGFGCAAPPAHTSFAA
                     SPADPAIRRVVPEDAEAVRLSFAPLVRAAAPAVVSVTARRTAVQSEADTFYDDPIFRH
                     FFGDLGRFGRGRPRQQREQASLGSGVIVRADGLIVTNRHVIEGADAITVVLGDRREFP
                     AEVVLSDELSDLGFLRIEPGQDLPVLPLGDSDRIEVGDLVLAIGNPFGVGQTVTSGIV
                     SAAARATPGGRSDVSFIQTDAAINPGNSGGALVSMDGELIGVNTAIFTRGGGGSIGIG
                     FAIPANLVAARLAGIEQGTGTERPWLGAEYAPIDNSTADALGLARPQGVLIERVHPKA
                     AGAAAGLRPGDVVTAVGGVEINDAPGLNFRLALEPLGQTVPLTVLRRGERLTLDLPLR
                     PAPAEPPADRRTLDGNQPLSGTTVANLSPALSQGLGLDPFDSGVVVLGIERGSFATRL
                     GLRPGDAIHAVNGEVVASTAELERMLDENARRWVVTVRRDGRLFDLTIS"
     gene            15332..16636
                     /locus_tag="P5558_09315"
     CDS             15332..16636
                     /locus_tag="P5558_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRY24548.1"
                     /translation="MPAKRRATAHAPLAERLRPQTLEDVVGQDAALAADSPLGRMLAH
                     KNLGSLILWGPPGCGKTTLARLLAREVGQELLPLSAVMSGVAELRKAFESARRLAAAG
                     RRPVLFIDEIHRYNRAQQDGLLHEVEAGTVSLIGATTENPSFALNPALISRCLVVVLD
                     RLDEAALLQLLDRAERAIGHALPLEPAARARLAELADGDGRYLLNMAESLADLPAEPA
                     LDVEAMGHVLSRRLPIYDKQQEAHYNLISALHKSVRGSDPDAALYWLCRMLEGGEDPR
                     YIARRMIRMANEDIGLAQPEAVVQAVAAAEVYERLGSPEGELALAQVVVHLALAPKSN
                     AIYRAFGAARRSAKEHGSLMPPAHILNAPTKLMKALGYGQGYAYDHDAPDHFSGQGYF
                     PEGMERPSFYHPTEEGLEKRLKEKLQHLAGLRRARGSGEGGA"
     gene            16633..17613
                     /locus_tag="P5558_09320"
     CDS             16633..17613
                     /locus_tag="P5558_09320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY24549.1"
                     /translation="MSGVTHRVVAAGEADLRFDRWCKRHFPTLGHVQLQKLLRTGQFR
                     LDGKRVEAGTRVQPGQTIRVPPMELAAAAERRGPSEADARFIKSLVIHDDDRLVVLAK
                     PEGLAVQGGTKTTRHVDGMLDALARKGERPKLVHRLDRDTSGLLVLARDATVARELMH
                     AFQQHRVRKLYWAIVQGSPDRNEGLIDLGLGKAGAKGFERMTPDALDARRAQTDFRVI
                     ARTGKVAAWVGLMPRTGRTHQLRAHMAAIGTPIVGDRKYAPVAGREQPATAPEGLMLH
                     AREIELPRGKGPPLRLTAEAPRHFREGLAWLGLDAEALKFSRLQDWPEEG"
     gene            complement(17589..18203)
                     /locus_tag="P5558_09325"
     CDS             complement(17589..18203)
                     /locus_tag="P5558_09325"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRY24550.1"
                     /translation="MGDLAERRMTLAEFLVWDDGTDTRYEFLRGQPRAMAPASGRHGV
                     IQRNAGRAIEARIGLQGPCRVVDQAGILLTVRGDERFYVPDLALTCRPVTASPYIDEP
                     KLIVEVASPSTGRTDKDVKVPDYCALLSVDEVWLIQSQRPLMQVWQRLDGQWIGSLPL
                     RLGQGFASRLLGGEVRVDDIFAGTGLELPEEEPADADQPSSGQS"
     gene            18272..18919
                     /locus_tag="P5558_09330"
     CDS             18272..18919
                     /locus_tag="P5558_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY24551.1"
                     /translation="MTQLIVFDCDGTLVDSQAVIVACAQDAFAAVGLVAPSVERIRHI
                     VGVSLEPAMRMLLGRDDPEMGRRIAEAYREAFFARRAMPDHDEPLFEGVLETLDALAA
                     RGAVMGVATGKTMRGLVQILERHALERYFVTLQTADLHPSKPHPSMLEAAMRETGAAA
                     DATWIIGDTTWDIEMGRLAGCRSIGVSYGNHAPEVLRTAGAAHVIDRLNDLLDLV"
     gene            18916..>19545
                     /locus_tag="P5558_09335"
     CDS             18916..>19545
                     /locus_tag="P5558_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HRY24552.1"
                     /translation="MKRFYKACTTGSGEGGHTVLLDDRPIRTPGRRLLALPTSALAEA
                     VASEWAAQGETIRPESMGVTRLASTVTDQLPRRRDDAIAEIQGYAEADLLCYRTGAPS
                     DLAERQAARWQPWLDWAARRLDAPLLVTTAIDPLPQPSASLEALRRATTRLDDWHLAG
                     LHATTRLTGSIVLGQAMLMGELDPATAFDLALLEELFEIERWGLEAEQAR"
BASE COUNT         3310 a         6628 c         6772 g         2835 t
ORIGIN      
        1 gcaccgtcgg cgccggcgtc gtcgccgaaa tcatcagctg aaaccgatag cgggttagcg
       61 ggacaggtcc gtcatggaaa cgcagaacat cagaattcgc ctgaaggcgt tcgatcatcg
      121 cgtgctcgac cagtcggtgc gcgagatcgt cggcacggcg aagcggaccg gtgcgcgggt
      181 gcgcggcccg atcccgctgc ccaaccgaat cgagcggttc accgtgaacc gtggaccgca
      241 cgtcgacaag aagagccgtg agcagttcga gatccggacc cacaagcggc tcctcgacat
      301 cgtcgacccg accccccaga ccgtggacgc gctgatgaag ctcgacctcg cctcgggtgt
      361 cgacgtcgag atcaagctct gaggcgaatt cgatgcgaac ggggatgatc gcccggaaga
      421 tgggcatgac gaggctcttc ggggtcgacg gcagccacat tccggtcacc gtgctcaagg
      481 tcgacgactg tgaggtcgtg gccgtgcgga cggtcgagaa ggatggctac accgccgtcc
      541 agctcggtgc cggcaaggcg aagccgaaga acgtcaccaa gccgatgcgc ggccatttcg
      601 ccaaggcgaa ggtcgagccc aaggccgagg tcgccgagtt ccgggtggcg gaggatgcct
      661 tgctcggggt cggcgagagg gttcatgtcg gccacttcgt cgccgggcag ttcgtcgacg
      721 tcgccgggct gagcatcggc aagggcttcg cgggtgcgat gaagcggcac aatttctccg
      781 gtctcagggc cagccacggc gtgtcggtgt cgcatcgcag ccacggctcg accggccagc
      841 ggcaggatcc gggccgggtg ttcaagggca agaagatggc cggccacatg ggccatcgca
      901 atgtgacggt gcagaacctc gaggtcttcg gcgtcgatcc ggaccagggc ctgatcctca
      961 tccggggcgg tgtcccgggt gccaagggca gctggcttcg ggtcaccgac gccgtgaaga
     1021 aggccctgcc gaaggatgcg ccgttcccgg cggccgtggt cagggccggc gaggcgatgg
     1081 aagccatcgt cgccgaagag ggcggggaat agtcatgcag gtcgacgtca agaacctggc
     1141 gggcgaggtg gtcggctcta ccgagctgaa cgacgccgtc ttcgccgtgc cggtgcgccg
     1201 cgacatcctg caccgggtgg tgacctggca gctcgccaag cgccgcgccg ggacccacaa
     1261 gatcaagacc cggggcgagg tcagtggctc gacggccaag atctacaagc agaaggggac
     1321 gggccgcgcg cgccacggca gcaagcgggc caacatcttc cgcaccggtg gtcgcgcctt
     1381 cgggccgacg ccgcgcgatc acagcaccga cctgaccaag aagctgcgga agctcggcct
     1441 gcgctgcgcg ctctcggcca aggccggggc cgggcagctc atcgtcctgg acgaggcggc
     1501 gctgaccgag cccaagacca ggctgatggg cggcgtgctc gacaagctgg gcctcggctc
     1561 cgctctcttc gtggtggccg gcgagcccga gcgcaacttc gcgctcgcct cgcgcaatct
     1621 gccgaccatc gacctgctgc cgcaggaggg tgccaacgtc tacgacatcc tgcggcgcga
     1681 cgtgctggtc ctgacccggg acgcggcgaa gctgctcgag gagcgcctag catgacgacg
     1741 gcgcgcaatt acgacgtgat cgtggccccg gtgatcaccg agaaggcgac ccgggtctcc
     1801 gagaacggcc aggtggtctt caaggtccgg ctcgacgccg acaagggcga gatcaaggaa
     1861 gccgtggaga gcatcttcgg cgtcaaggtg acggcggtga acacgctcgt gcagaagggc
     1921 aagaccaagc ggttccgcgg ccgaccgggc aagcggaacg acatcaagaa ggcgttcgtc
     1981 acgctggccg atggccaggc gatcgacgtg acgaccggca tctgagggac cggcgatggc
     2041 gctcaagcag tacaacccga ccagtccttc gcggcgacag ctcgtcaccg tggaccgctc
     2101 tgccctgtgg aagggcaagc cggtcaagaa gctgaccgaa gggctgacca agtccggcgg
     2161 ccgcaacaat cacggccgca cgacggcgcg ctatatcggt ggcgggcaca agcgcgccta
     2221 tcgcatggtc gacttcaagc ggcgcaagtt cggcgtcaac gggacggtcg agcggctcga
     2281 gtacgatccc aaccgcacgg cgttcatcgc gctgatcggc tacgaggacg gcgagcaggc
     2341 ctacatcctg gcaccgcagc ggctgcaggc cggcgacaag gtgatcgccg gcgagcgggt
     2401 cgacgtgaag ccgggcaacg cggccccggt ccgcgccatc ccgatcggca cggtgatcca
     2461 caatgtcgag ctgaagccgg gcaagggcgg ccagatcgcc cgttcggccg gctcctacgc
     2521 gcagatcgtc ggccgcgacg gcgactacac ccaggttcgc ctgggctcgg gcgagatccg
     2581 ccgcgtgcat tcgacctgca tggcgagcat tggcgcggtg tcgaaccccg atcaccagaa
     2641 catcaattac ggcaaggctg gtcgcaagcg ctggctgggc aagcggccgc atgtccgtgg
     2701 tgtcgccatg aacccggtcg atcacccgca tggcggcggc gagggccgca cctcgggcgg
     2761 ccggcatccg gtgacgccct ggggcaagga caccaagggc acgcgcaccc ggagcaacaa
     2821 gaagacgcag ggcatgatca ttcgcagccg ccacaaagcc aagaagagag gaggctgagc
     2881 atgccccgtt ccgtctggaa gggcccgttc gtcgacggct atctcttcga caaggtcgag
     2941 aaggcgcggt cctcgggccg caacgagatc atcaagacct ggtcgcgacg ctcgacgatc
     3001 atgccggatt tcgtcggcct gaccttcgcc gtctacaacg gcaagaaatt cgtgccggtc
     3061 tacgtcaccg agaacatggt cgggcacaag ctcggcgagt tctcgccgac gcgcacctat
     3121 acggggcacg gcgccgacaa gaaggcgaga aggaaatagc catgggcaag ccgaagcagg
     3181 agcggcgcct cgccgacaac gaggccatgg cggtcgccac cctcgtccgc tcgagcccgc
     3241 agaagctcgg cctcctggcc gggatgatca acgggatgaa ggtggacaag gcgctggtgg
     3301 cgctcgagtt caatcgcaag cgcgtgtcgg aggtggtgtt caaggcactg cagtcggccg
     3361 tggccaatgc cgagaacaac cacgcgctcg acgtcgaccg gctctacgtc gccgaggcga
     3421 gtgtcggcaa gcggctcgtc atgaagcgct tcaagccgcg tgcccgtggc cgggccggtc
     3481 gcgtcgtcaa gcatttcagc cagctgcgca tcgtcgtgca ggagcgggcc gagcaagagg
     3541 aggccgcctg atggggcaga aggtcaatcc ggtcggcctg cgcgtcggca tcaaccgcac
     3601 ctgggattcg cgctggtacg ccgatcgcga ctacgcccag ctgctcgccg acgacgtcga
     3661 gatccgccgc ttcctgatga agcggctggc ccaggcgggt gtcagccgca tcgtcatcga
     3721 gcggccggcg aagaaggccc gggtcaccat ctactcggcg aggcccggcg tggtcatcgg
     3781 caagaagggc caggagatcg agaatctccg gcgcgacctg gcgaagctct gcggtggcga
     3841 ggtgcatctc aacatcgtcg agatcaggaa gccggagctc gacgcgcggc tggtcgccga
     3901 gaacatcgcc cagcagctcg cccgccgggt cacgttccgg cgcgccatga agcgggccgt
     3961 gcagtcggcc atgcggctcg gcgcccaggg catccgggtg acctgcggcg gtcgtctcgg
     4021 cggtgccgag attgcgcgga cggagtggta tcgcgagggc cgggtgccgc tgcacacgct
     4081 gcgcgccaac atcgatttcg gcaaggcgac gtcgcacacg ccctatggca cgtgcggcgt
     4141 gaaggtctgg gtgttccgcg gcgagatcat ggagcacgac ccgatggcct tcgaccaacg
     4201 cttcaacgag cagaatccgg gacgcgcctg agggcttggg aattcagaga tgttgcagcc
     4261 gaaacgaacc aaattccgca aggcccacaa gggccgcgtg cgcggcatga ccaaaggcgg
     4321 ggccgagctg aacttcggcg ccttcggcat gaaggcgacg gaaggcgggc ggatcacggc
     4381 gcgccagatc gaggcggcgc ggcgggcgat cacccgccat ctgaagcgtg ccggccgggt
     4441 ctggatccgg atcttcccgg ccaagccggt gtcgaagaag ccggccgagg tgcgcatggg
     4501 caagggcaag ggctcggtcg agttctgggt ggcccccgtg cagcgcggcc gcatcatgtt
     4561 cgagatcgac ggcgtgccgc atcagctcgc cgaggaggcg cttcagcgcg cctcggccaa
     4621 gctgccgatc ggcacccgct tcgtgcaccg agtgcaggag gacgcggcat gaacgcgagt
     4681 gacatgcgcg ccgagacagt cgaccagctc agcgatcggt tgctgcagct gaagaaggaa
     4741 cagttcaacc tgcgcttcca gcgcgccacc ggccagctcg agggcacggc gcggatgagc
     4801 gtggtgcgac gcgacattgc ccgcatcaag acggtgctgc acgaacggcg tgccgccggc
     4861 ggcgcgggct aggagaggac catgccgaag cgcgtgttgc agggtgtcgt ggtcagcgac
     4921 aagggtgaga agacggtgat cgtcaaggtc gagcgccgtt tcatgcaccc gctctacaag
     4981 aagtatatcc ggcgctcgaa gcgctacgcg gcgcacgatg agagcaacgc ggtgccgatc
     5041 ggcgccaagg tgcgcatcga ggaatgccgc ccgctttcca agaccaagcg ctggttggtg
     5101 atcggtgacg gttccgccac cgaagcggct ccagcgagcg aataggggcg ggggatgatc
     5161 caggtcgaga ccaatctcga ggtcgccgac aattcgggtg cgcgccgcgt gcagtgcatc
     5221 cgggtgctcg gtggctcgca tcgccgctgg gggtcggtcg gcgacatcat cgtcgtcgcc
     5281 gtcaaggagg cgatcccgcg cggcaaggtg aagaagggtg acgtgcatca tgccgtcatc
     5341 gttcgcaccg ccaaggagat ccaccgggcc gacggctcgg cgatccgttt cgacaagaac
     5401 gcggcggtgc tcatcaacaa gcagggcgag ccgatcggca cgcgcatctt cggccccgtg
     5461 acgcgcgagc tgcgtgccaa gcggttcatg aagatcgtca gcctcgcccc ggaggttttg
     5521 tgatggccgc gcgtattcgc aagggcgacc gggtggtggt gatcgccggc aagtcgaagg
     5581 gggtggaagg cgaggtcctg aaggtcctgc cggccgagaa ccgggtggtg gtccagggtg
     5641 ctcagcaggt gaagaagcac accaagccga gtgctcgtgg ggccggcggg atcgagagcc
     5701 gcgaggcgcc gatccaagtc tccaatgtag cgcacatcga tcccacggac ggcaagccga
     5761 cccggatcgg gttcaagttc ctcgatgacg gccgcaaggt ccgcttcgcg aagcggtccg
     5821 gcgagatcat cgatcgctga ggatgaggtg atgacggctc gtttcaagca gcgctacgac
     5881 gaggtggtgc gtggccaact gcaggagcag tacggctaca agaacgtcat gcaggtgccg
     5941 cggctggaga agatcgtcat caacatggcg gtcggcaagg ccgtcacgga cagcaagaag
     6001 atgaccgccg cggtgggcga tctcaccaag atctccgggc agaagccggc ggtctgccgg
     6061 gcgaagaagt cggtcgccaa cttcaagctg cgcgaaggca tgccgatcgg caccaaggtc
     6121 acgctgcgca gccagcggat gtacgagttc ctcgaccggc tggtgacgat cgccctgccg
     6181 cgggtccggg acttccgcgg catctcgccc aagagcttcg acggccgggg caactatgcc
     6241 ttcggcctga aggagcagat cgtcttcccc gagatcaatt acgacgaggt cgacgagatc
     6301 cggggcatgg atatcatcat ctgcaccacg gccaggaccg atgcggaagc gctgtcgctt
     6361 ctcaaggggt tcgacatgcc gtttcggacc tgacggagag gatcgcccaa tggccaagaa
     6421 gagtgccatc gagaagaaca agcgccgtcg ggagatggtc gctcagcagg ccgccaagcg
     6481 ggcggcgctg aaggccgtga tcgccaaccg cgagggcgac cccgaggtgc gtttcgaggc
     6541 cgtgctgaag ctcgccgccc tgccgcggaa cggcgccagg gtgcgcatcc gcaaccgctg
     6601 cacgctgact gggcggccgc gggcctatta ccgcaagttc ggcatgtcgc gcatcgcgct
     6661 tcgggacctc gcctctgaag gccaggtgcc cggcgtggtg aagtcgagct ggtgagccgg
     6721 gagtactgaa atggcgatgt ccgacccact cggcgatatg ctgacccgga tccgcaacgg
     6781 gcagatggcg cagaaggcga gcgttcgctc gcctgcgtcg aacctgcggc ggaacgtgct
     6841 cgaggtgctg cagcgcgagg gctatattcg cggctacgtc gccgacgagg tgatcgacgg
     6901 caagtccgag atctcgatcg agctgaaata tcacaatggc gagccggtga tccgggagct
     6961 tcgccgggtg tccaagcccg gccggcggat ctatgccggt gtgcgcgagc tgccggagat
     7021 ctacaacggt ctcggcatcg ccatcctgtc gaccccacgg ggcgtgctgt ccgattccga
     7081 ggcccgcgag gccaaggtcg gcggcgaagt cctctgcacg gtattctgat catgtctcgt
     7141 atcggcaagc atccagtcgc gattccgggg ggcgtcgaca tcgcggtcga cggccagacg
     7201 gtcaaggtca agggcaagct cggctcgctc gaggaaaccc tgcccggcga ggtggtggtg
     7261 gcgctcgagg acggctcgct cgcggtccgg ccgcgcgacg agagcaaacg cgccgcgcag
     7321 atgtggggcc tggcgcgcac gctcgtgcag aacatggtca ccggcgtcag cgagggcttc
     7381 acccgtcgtc tggatatctc cggtgtcggc taccgcgctg ccgtggacgg caacattctg
     7441 aacctgcagc tcgggttcag ccacgacatc aagttcgcgg tgccgccgga ggtgaagatc
     7501 gcctgcgaga cgccgacggc gatcgtgatc tccggcttcg agcggcagcg ggtcgggcag
     7561 atcgcggcgg agatccgctc gttccgcccg ccggagccct acaagggcaa gggtgtccgg
     7621 tattccgacg aggtcgtccg tcgcaaggaa ggcaagaaga agtagccatg ctgaaagcca
     7681 gtgaacgatt ctcgcgtcgg cagcggcgca cgcgcttcgc gcttcggcag gccgccaacg
     7741 gccggccgcg gctctccgtt ttccgctcgg gcaagcacat ctacggccag atcatcgacg
     7801 atgaggccgg ccgcacgctg gtcgccgcgt cgactctcga gcagccgctg cgtggcgagc
     7861 tgaagaccgg cgccgacatg gcggcggcgc agcgcgtcgg caagctgctc gccgagaagg
     7921 ccagggcggc cggggtcgaa tcggtggtgt tcgatcgtgg cggctacaag tatcatggtc
     7981 gggtcaaggc gctcgccgag gcggcgcgcg aaggcggcct gacattttga gcgcggtata
     8041 gggaattcca atggcacgtc cacagcgcgg tgaagatcgc caggattcag agctcgtcga
     8101 ccggctggtc aacgtcaacc gcgtcgccaa ggtcgtgaag ggcggcaggc gcttcggctt
     8161 ctcggcgctg gtggtggtcg gcgacggcaa gggccgggtc ggctacggca gcggcaaggc
     8221 gcgcgaggtg ccggaagccg tcaggaaggc gaccgagcag gcgaagcgcg gcatgatccg
     8281 cgtgccgctg cgccagggcc gcaccctgca tcacgacatc gtcgggcatt tcggtgccgg
     8341 ccgcgtgctg ctgcgcgccg cgccgcccgg caccggcatc atcgccggcg gcccgatgcg
     8401 cgccgtgctc gaggccctgg gcgtcaagga cgtcgtctcc aagtcgctcg gcacctcgaa
     8461 tccgcacaac atggtgaagg cgaccttcga ggcgttgcag ctcgtcaact cgccgcgcac
     8521 cgtcgcggcc aagcgcggca agaacgtgcg cgacatcatc ggcaagcgcg agcccggcgc
     8581 cgaagcgacg gcggaggcct gaggcgatgg cgaagaccag caagaccgcc aagacgatca
     8641 aggtgaccca gaccggcagc cccatcggcc ggccggcgga tcaggagcga accctgatct
     8701 cgctcggcct gaacaagctg tggcggagcc gcgaggtcac cgacaccccg gccaatcgcg
     8761 gccggatccg caaggtcgaa cacctcctgc gcgtcgaatc cgacggcgcc tgaggtcgga
     8821 gcagaactga tgcgtttgaa cgaactgcgc gacaatcccg gcgcccgcta caaggcgaag
     8881 cggctcggcc gtggcatcgg ctccggcaag ggcaagaccg cgggcaaggg tcacaagggc
     8941 cagaaggctc gcagcggcgg cgccgtgcgg gtgttcgagg gcggccagat gccgatctat
     9001 cggcgcctgc cgaagcgcgg cttcaagaat gtgaaccgcc agagcttcgt cgtcgtcaat
     9061 ctcggcacgc tgcaggcagc gctcgatgcc ggcaagctcg acgcggcgac gccgatcgac
     9121 gcgggccggc tggtcgagac cggggtgctc cggcgcacgc tcgacggggt gcggctgctc
     9181 gccaagggcg agctcaaggc ggcggtgacc gtcgaggtca cgggggccag caaggccgcg
     9241 atcgccgccg tcgagaaggc gggcggcaag gtggtgctgc tcggcggggc gccggtcgaa
     9301 gcggctgccg acaccggcag cgaggcctga ggggacgcgc atggcgtcgg tcgccgacca
     9361 actcgcgtcg aacatcaatt tcggcgcctt cgccaaggcc accgagctca agcggcggat
     9421 ctggttcgcg gtcggctgcc tgatcgtcta tcggatcggc acctacatcc cgctgccggg
     9481 catcgacccg caggtgatgg cgcagatctt cgcgcagcag gccggcggca tcatgggcat
     9541 gttcaacatg ttcgccggcg gcgccctcga gcgcatgtcg atcttcgccc tcggcatcct
     9601 gccctacatc tcggcctcga tcatcatgca gctcatgacg gcgatctcgc cgtcggtggc
     9661 ccagctcaag aaagagggcg aggccggtcg caagaagatc aaccaataca cgcgctacct
     9721 caccgtcctg ctcgccgccg tccaggccta cggcctctcg gtcgggctcg agtcgatggg
     9781 cgggccgggc ggcagcgccg tgatcgagcc gggctacttc ttccgcacct cgacggtgat
     9841 caccatcgtc ggcggcacga tgttcctgat gtggctcggt gagcagatca ccgcgcgcgg
     9901 catcggcaac ggcatctcgc tgatcatctt caccggcatc atcgcgaccc tgccgagcgc
     9961 gctcgccagc atgctggagc tcggccgcac cggtgcgctg tcgccgctct tcatcatcgc
    10021 catcctcatc ggcggcctcg ccgtcatcgc cttcatcgtc ttcatggagc gcgcccagcg
    10081 acggatcatc gtccagtatc cgaagcgcca gcagggcaac aagatgttcg gcggcgagag
    10141 ctcgcatctg cccctgaagc tcaacacctc gggagtcatt ccggcgatct tcgccagctc
    10201 cctgctgctc atgccggcga cgctcggcag cttctcgggt ccgggcgcgc cggaatggct
    10261 gctggtcgtc agtgccctgc tcggccgcgg tcagccgctc ttcatcctga tcatggtcgg
    10321 gttgatcgtc ttcttcgcct tcttctacac ctcgatcgtg ttcaacccgc aggagacggc
    10381 cgacaacctg aagaagcatg gcggcttcat ccccggcatc cggccgggcc agcgcacggc
    10441 cgagtatctc gactacgtgc tgacccgcct gaccaccgtg ggtgcgctct atctcgccgc
    10501 ggtctgcgtg atgccggaga tcctgatctc gcagtactcg gtcccgttct acttcggcgg
    10561 caccagcctg ctgatcgtgg tcgccgtcac catggacacg gtggcgcaga tccagtcgca
    10621 cctgatcgcg caccagtacg agggcctcat caagaaatcc cggttgcgcg ggaggcgtgg
    10681 ctgatgaacc tcgtgctgtt cggcccgcca ggtgccggca aggggaccca ggcagcgcgg
    10741 ctgcgtgatg gccacggctt ggcgcacctc tcgaccggcg acatgctgcg ggccgcggtc
    10801 gaggccggca ctcccatcgg ccgcgaggcc gagagcgtga tggcctcggg caggctcgtc
    10861 tcggacgagc tgatctgcgg cgtcgtcgcc gagcggatcg atgcgccgga ctgcgccaac
    10921 ggcttcatcc tcgacggctt cccgcgcacc ctggcccagg ccgaggcgct ggatgcgatg
    10981 ctgcggggca agggcaaggc gatcgacgcg gtgatcgaga tccgcgtgga cgaggcggtg
    11041 ctggtcgagc ggatcaccaa ccgcatccag gaaaccggcg gggcccgcgc cgacgacaat
    11101 gtcgagacgc tgaagaagcg gctcgaggtc taccgggcgc agaccgcgcc cattctgccc
    11161 ttctatgccg aaaagggcat cctccacacg gtcgacggca tgcagccgat ggaccgggtg
    11221 gcggccgaga tcgaggcggc gctcgccccc gggcgcgcct ccggcacctg agccgaggcc
    11281 tggcgtcggt gcctgcgaca ggacgtcgcg aagcgccgat tgacaggccg aggtcgggaa
    11341 ctatactccg gcggctctgg gctggaactc cagcccggca ccgcttgcac gttgtcttcg
    11401 caagcttggg agaacctctg gtggcgcgta ttgccggtgt caacgttccg gcccagaagc
    11461 cggtcgggat cgccttgacc tatatctatg gcgtcggccg cacgacgtcc gagaagatcc
    11521 tccagcagtg cgaggtgccg ttcgagcggc gcgtcaacac gctgagcgat gccgagctcg
    11581 cgcgcatccg cgaggcgatc gaccgcgacc tgaaggtcga gggcgatctc cgccgcgaga
    11641 tggcgatgag catcaagcgt ctcatggacc tcggctgcta tcgcggcctc cggcaccgca
    11701 agggcctgcc ggtccgtggc cagcgcaccc ataccaatgc ccgcacacgc aaggggccgg
    11761 ccaaggcgat cgccggcaag aagaaggtga agaagtagac catggcgcag gacaccaagc
    11821 ttcgacgccg cgagcgcaag aacatcagct ccggcatcgc ccacgtcaac gccacgttca
    11881 acaacaccat gatcaccatc gccgacgcgc agggcaacgc gatctcctgg tcgagcgccg
    11941 gtagccaggg cttcaagggc tcgcgcaagt cgacgccgtt cgccgcgcag atggccgccg
    12001 aatcggccgg caagaaggcg atggagcacg gcatgcggac catcgaggtg ctggtcaagg
    12061 ggccgggctc gggccgcgag tcggctctcc gggcactgca ggccgtgggc ttcaccgtga
    12121 ccaacatccg cgacgtcacg ccgatcccgc acaatggctg ccggccgcgc aagcgccgcc
    12181 gcgtctgaca ctttcgttcg cgaccccaaa cggtcgccga ccgccaccag ctggcgtggc
    12241 gaccttcgtc atcatggcct cgatatcatg gcctcgtcct cacggcccgg tcgtcacagg
    12301 gcgtcacacc gacttgcgag gtagctcgtg atccaaagaa actggcagga gctgatcaag
    12361 ccgcagaagc tcaacatcca gcccggacgg cggccggaca gggaagcgac gatcatcgtc
    12421 gagcccctgg agcggggttt cggcatcacc atcggcaacg cgctccggcg cgtgctgctg
    12481 tcgtcgctgc agggtgccgc cgtctcggcc atccagatcg acggcgtgct gcacgagttc
    12541 tcgtcgatcc ccggcgtgcg ggaggacgtc accgacatcg tcctgaacct gaagtcgatg
    12601 ggcgtccgtc tgcacagcga cgtgccgaag cgggtctacc tcaaggccga gggcccgaag
    12661 gtggtcaccg ccggcatgat cgagaccacc cacgacgtcg agatcatgga tcccgaccag
    12721 gtgatctgcc acgtcgacga gggtggcaag gtctcgatgg agctgaccat ctccggcggc
    12781 aagggctacg tcccggcgca tctcaacaag tccgaggatg cgccgatcgg gctgatcgcg
    12841 atcgacgcca tctacagccc ggtgcgcaag gtgatgtacc gggtcgacaa tgcccgcgtc
    12901 ggccagcaga cggactatga ccggctggtc atgcaggtcg agaccgacgg ctcggtgagc
    12961 tcggacgacg cggtggcgct ggccgcgcgc atcctccagg accagctcgg gccgttcatc
    13021 aacttcgacg agccgcatgt cggccaagcg gcggagcggc gccccgagct gccgttcaac
    13081 ccgaacctcc tgcgcaaggt ggacgagctg gagctgtcgg tgcgctccgc caactgcctg
    13141 aagaacgaca acatcgtcta tatcggcgat ctcgtgcaga agaccgaggg cgagatgctc
    13201 cggaccccca acttcggccg caagtccttg aacgagatca aggaggtgct ggcgcatatg
    13261 gggctccatc tgggcatgga cgtcgccggc tggccgcccg agaacgtcga ggagctggct
    13321 cgcaaggtcg aagaaccgtt ttgatgagga ggccggcgcg gcaacgcgcc gggtaacgag
    13381 cgatgcgtca cgggaacagc ggccgcaagc tgaacaggac ggcgagccac cgccaggcca
    13441 tgttccagaa catggtccag gcgctggtca agcacgagca gatcacgacg accctgccga
    13501 aggcgaagga gctgcggccg atcgtcgagc ggttgatcac gctcggcagg ggcggcgacc
    13561 tgcacagccg ccggctgctg atcgcccggc tgcgctgcga aacgacggcg ggcaagatca
    13621 tggacgtgct cgccccgcgc tacaaggagc gcccgggcgg ctatgtgcgg atcatgaagg
    13681 ccggcttccg ctacggcgac aatgcgccga tggcggtgat cgagctggtc gatcgcgacg
    13741 tcgacgccaa gggccaggac tcggggccgg tgcagcacga tgacgacgag ggcgaggcag
    13801 tcgctgcctg attgcggccg ggcgccgctc gcgcgcgccc tgctcgtcgt tctctgcctc
    13861 gtcgccgggt tcggctgcgc ggcaccaccc gcccatacga gctttgccgc gtcgcccgcc
    13921 gatccggcga tccggcgggt cgtgccggag gacgccgagg ccgtccgcct ctccttcgcg
    13981 cccctggtgc gcgctgcggc accggccgtg gtcagcgtca cggcccggcg gacggcggtc
    14041 cagagcgaag ccgacacgtt ctacgacgac ccgatcttcc gccacttctt cggcgatctc
    14101 ggccggttcg gccggggccg gccgcgccag cagcgcgagc aggcttcact gggctcgggc
    14161 gtcatcgttc gggccgatgg gctgatcgtc accaatcgcc acgtcatcga gggcgcggat
    14221 gcgatcaccg tggtgctcgg cgaccggcgc gagttcccgg ccgaggtcgt gctctccgac
    14281 gagctgagcg atctgggatt cctgcgcatc gagcccggcc aggacctgcc ggtcctgccg
    14341 ctcggcgatt ccgacaggat cgaggtcggc gatctcgtgc tggccatcgg caacccgttc
    14401 ggcgtcggcc agaccgtgac ctccggcatc gtctcggccg cggcccgggc cacgcccggc
    14461 gggcgcagcg acgtctcctt catccagacc gatgccgcga tcaaccccgg caactccggc
    14521 ggtgcgctcg tctccatgga cggcgagctg atcggcgtga acaccgccat cttcacccgc
    14581 ggcggcggcg gctcgatcgg catcggcttc gccatcccgg ccaacctggt cgcggcgcgg
    14641 ctggccggga tcgagcaggg caccggcacc gagcgaccgt ggctcggggc ggagtatgcg
    14701 ccgatcgaca acagcacggc ggacgcgctc ggcctcgccc ggccgcaggg ggtgctgatc
    14761 gaacgagtcc atcccaaggc cgccggcgcc gccgcgggcc tgcgacccgg cgacgtcgtg
    14821 acggccgtcg gcggcgtgga aatcaacgac gcgcccggcc tcaatttccg ccttgcgctg
    14881 gagccgctag gccagaccgt gccgctcacc gtgctgcgtc gtggcgagcg cctgacgctg
    14941 gacctgccgc tccggccggc cccggccgag ccgcccgccg atcggcgcac gctcgacggc
    15001 aaccagccgc tcagcggcac caccgtcgcc aacctctcgc cggccctgag ccagggcctc
    15061 gggctcgatc cgttcgattc cggggtcgtt gtgctcggga tcgagcgcgg ttccttcgcg
    15121 acccggctgg gcttgcggcc gggcgacgcg atccatgccg tcaacggcga ggtggtggcg
    15181 agcacggccg agctcgagcg gatgctcgac gagaacgcgc gtcgctgggt cgtgaccgtc
    15241 cgccgcgacg gacggctctt cgatctgacg atcagttgag cggcggccga tcgccatgac
    15301 cgaggccgat ctcttcgcga ccgagccgcc gatgccggcg aagcgcaggg ccacggccca
    15361 tgccccgctc gccgagcgcc tgcggccgca gaccctggaa gatgtggtcg ggcaggacgc
    15421 ggcgctcgcc gccgattcgc cgctcggccg gatgctcgcc cacaagaacc tcggctcgct
    15481 catcctctgg ggtccgcccg gctgcggcaa gaccacgctc gcccgcctgc tggcccggga
    15541 ggtcggccag gagctgctgc cgctctccgc cgtcatgtcc ggcgtcgccg agctcaggaa
    15601 ggcgttcgag agtgcccgtc gactggccgc cgccggtcgc cgcccggtgc tcttcatcga
    15661 cgagatccac cgctacaatc gggcccagca ggacgggctc ctgcacgagg tcgaggccgg
    15721 cacggtgagc ctgatcgggg cgaccaccga gaacccgtcc ttcgccctca acccggcgct
    15781 catctcccgc tgcctcgtgg tcgtgctcga ccgcctcgac gaggcggcgc tgctgcagct
    15841 cctggacagg gcggagaggg cgatcggcca tgccctgccg ctcgagccgg cggccagggc
    15901 acggctcgcc gagctcgccg atggcgacgg ccgctatctt ttgaacatgg ccgagagcct
    15961 ggccgacctg ccggccgagc cggccctcga cgtcgaggcc atgggccatg tgctcagccg
    16021 ccgcctgccg atctacgaca agcagcagga ggcgcactac aacctcatct cggccctgca
    16081 caagtcggtg cgcggctccg atcccgacgc ggcgctctac tggctctgcc ggatgctcga
    16141 gggcggcgag gacccgcgct acatcgcgcg gcggatgatc cgcatggcca acgaggatat
    16201 cggcctggcc cagcccgagg cggtcgtcca ggcggtcgcc gcggccgagg tctatgagcg
    16261 gctcggcagc cccgagggcg agctggccct ggcccaggtg gtcgtgcacc tggcgctcgc
    16321 ccccaagtcg aacgccattt accgcgcctt cggcgccgcc cgccgcagtg cgaaggagca
    16381 cggctcgctg atgccacccg cccacatcct caacgcgccg accaagctga tgaaggcgct
    16441 cggctacggc cagggctacg cctacgacca cgacgccccg gaccatttct ctggccaggg
    16501 ctacttcccc gaaggcatgg aacggccgtc cttctaccac ccgacggaag agggcctgga
    16561 gaagcgcctg aaggagaagc tccagcatct ggcgggcctg cgccgcgcgc gcggctcggg
    16621 ggagggcggg gcgtgagcgg cgtcacccat cgcgtggtgg ccgccggcga ggccgatctc
    16681 cgcttcgatc gctggtgcaa gcggcatttc cccaccctcg gccacgtcca gctgcagaag
    16741 ctcctgcgca ccggccagtt tcgcctcgac ggcaagcggg tcgaggccgg cacgcgcgtc
    16801 cagcccggcc agacgatccg cgtgccgccc atggagctcg ccgcggcagc cgagcgtcgt
    16861 ggcccgagcg aggccgacgc gcgcttcatt aagagtctcg tcatccacga tgacgatcgg
    16921 ctggtggtgc tggccaagcc cgagggtctc gccgtgcagg gcggcaccaa gaccacccgc
    16981 catgtcgacg gcatgctcga tgccctggcg cgcaagggcg agcggccgaa gctggttcat
    17041 cgcctcgacc gggacacgag cggcctgctc gtcctcgccc gcgacgcaac cgtggcgcgc
    17101 gagctgatgc acgccttcca gcagcaccgg gtgaggaagc tctattgggc catcgtccag
    17161 ggcagccccg accgcaacga ggggctgatc gatctcggcc tcggcaaggc cggggccaag
    17221 ggcttcgagc ggatgacgcc ggatgcgctc gacgccaggc gcgcccagac cgatttccgc
    17281 gtcatcgcgc gcaccggcaa ggtggcggcc tgggtcggcc tcatgccacg caccggccgc
    17341 acccaccagc tccgcgccca catggcggcg atcggtacgc cgatcgtggg cgatcgcaaa
    17401 tatgcccccg tggccggcag ggagcagccg gcgacggcgc ccgaggggct gatgctgcat
    17461 gcccgcgaga tcgagctgcc acgcggcaag ggcccaccgc tccgcctcac cgccgaggcg
    17521 ccccggcatt tcagggaggg tctcgcctgg ctcggcctcg acgccgaggc gctgaagttc
    17581 agccggcttc aggactggcc ggaggagggc tgatccgcgt ctgccggctc ttcctccggc
    17641 agctccagcc cggtgccggc gaagatgtcg tccaccctga cctcgccacc gagcagccgg
    17701 ctcgcgaagc cctggccgag ccggagcggc aggctgccga tccactgccc gtccagccgc
    17761 tgccagacct gcatcagcgg tcgctggctt tggatcagcc agacctcgtc gacgctcaaa
    17821 agcgcgcagt agtccggcac cttgacgtcc ttgtcggtcc ggccggtcga tggcgaggcc
    17881 acctcgacga tcagcttcgg ctcgtcgatg taaggcgagg ccgtcaccgg tcggcaggtc
    17941 agggccaggt ccggcacgta gaaccgctcg tcaccgcgca cggtgagcag gatgccagcc
    18001 tggtcgacca cgcggcatgg tccctgcagt ccgattcggg cctcgatggc ccggccggca
    18061 tttcgctgaa tgaccccgtg tcgtccgctc gcgggcgcca tggcgcgcgg ctggccgcgc
    18121 aggaactcgt agcgcgtgtc ggtgccgtcg tcccagacga ggaactcggc cagcgtcatc
    18181 cggcgttcgg cgaggtcgcc catcggcatc gtccccactt gacccgcgag aactatagcc
    18241 cgacctagca ggcgcaacca aacggtggat catgacccag ctcatcgtct tcgactgcga
    18301 cggcacgctc gtcgacagcc aggcggttat cgtcgcctgc gcgcaggacg ccttcgccgc
    18361 ggtcggcctc gtcgccccct cggtcgagcg catccggcac atcgtcggcg tctcgctcga
    18421 gcccgccatg cgcatgctgc tcggccgcga cgatcccgag atggggcggc gcatcgccga
    18481 agcctaccgc gaggcgttct tcgcccgtcg cgccatgccg gaccacgacg agcccctgtt
    18541 cgagggtgtt ctcgagacgc tcgatgccct ggccgcgcgc ggtgcggtga tgggtgtcgc
    18601 caccggcaag acgatgcgcg gcctcgtcca gattctcgag cggcatgcgc tggaacgcta
    18661 cttcgtcacc ctgcagacgg cggacctcca tccctcgaag ccgcacccct cgatgctgga
    18721 agcggcgatg cgcgagaccg gtgccgccgc cgacgccacc tggatcatcg gcgacaccac
    18781 ctgggacatc gagatgggcc ggctcgccgg ttgtcgctcg atcggcgtgt cctacggcaa
    18841 ccatgcgccg gaggtgctcc gcacggccgg tgcggcgcac gtcatcgacc ggctgaacga
    18901 cctgctcgac ctcgtatgaa gcgcttctac aaggcctgca caacgggcag tggcgagggc
    18961 ggccacaccg tgctgctcga cgaccggccg atccgcaccc ccggccgccg cctcctggcc
    19021 ctgccgacgt cggcattggc tgaggcggtt gccagtgaat gggcggcgca gggcgagacc
    19081 atccggccgg agagcatggg cgtcacccgg ctcgccagca ccgtgaccga ccagctaccc
    19141 aggcgccgcg acgacgccat cgccgagatc cagggctatg ccgaggccga tctcctctgc
    19201 taccgcaccg gcgctccatc cgacctcgcc gagcgccagg cggcccgctg gcagccctgg
    19261 ctcgactggg ccgcccggcg tctcgacgcc ccgctcctgg tgacgacggc catcgatccc
    19321 ctgccgcaac cgtcggcgtc gctcgaggcg ttgcggcgag ccaccacccg cctcgacgac
    19381 tggcacctcg ccggcctgca cgcgaccacg cgcctgaccg gctcgatcgt cctcggccag
    19441 gccatgctga tgggcgagct cgacccggcc accgccttcg accttgccct cctcgaggag
    19501 ctcttcgaga tcgagcgctg gggtctcgag gccgagcagg cccgc
//
LOCUS       DAOTFI010000073        19494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3364_length_19494_cov_7.768661, whole genome shotgun sequence.
ACCESSION   DAOTFI010000073 DAOTFI010000000
VERSION     DAOTFI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19494
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3364_length_19494_cov_7.768661"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="P5558_09340"
     CDS             complement(<1..727)
                     /locus_tag="P5558_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24553.1"
                     /translation="MSALSRPLARLLEKPWLWAFVGAALVWLATIPFTPGRGAGDVLT
                     AAFTFATFFVIVGIGQMFVITLGPGNVDLSIPATITLAGSVATKLMDGQDALIALGFV
                     AAMGCGLAVGVFNYALIRALRIPPIIATLSSSFIIQSTAIAYGRGLRIKPPPALAEFT
                     TARVLGMPVLAICVIVLAALMSVVLHRTIYGRSVIAIGQNQRAARLAGIAVDRTRLAT
                     YVMSALFASLCGYLLSAFSGGASL"
     gene            complement(724..1665)
                     /locus_tag="P5558_09345"
     CDS             complement(724..1665)
                     /locus_tag="P5558_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24554.1"
                     /translation="MTSTLLAPRTLRAILPAVSLALLLAAIFWLQPRTMSYTGLNLML
                     NLAIPIALATIAQMFVLCVNELDLSIGAYVGYVACVTATWLVESPPLGIAALMAGILV
                     YAGLGALIHLRNLPSIVVTLGMSFVWLGVAVLILPRPGGTSPEWIRWLMKLHTPVVPF
                     PIIAATVIAIVVHVALMRTAYGSILRGAGGNPRAIERAGWSLLKIKVVMFALAGVFGM
                     LAGMALIGLTTSGDANIAARYTLLSIAGAILGGCEFVGGRVSPAGAVIGAITLTLAGS
                     FLSFLRISPDWQIGATGAILILVLALRALINRAEERQ"
     gene            complement(1662..3101)
                     /locus_tag="P5558_09350"
     CDS             complement(1662..3101)
                     /locus_tag="P5558_09350"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY24555.1"
                     /translation="MTAPIVELDGVAKSFGAVRALAGVGLVVDAGTCLGLVGHNGAGK
                     STLMQILAGTLAPDRGRIGVAGQDLSRGYDVVTAQRHGIRCVFQELSLCPNLSVAENA
                     RVFHPSIRGMGWRRRAAELVVAGLDRIFPRHGIEPGEVVGDLSIARRQMVEIARAFSV
                     TDVPVRLVILDEPTSSLDALVAEQLLAFVRRFVAEGGSAILISHLLGEILETSDRVVV
                     MRDGLVVEARAAADFSRESLVAAMGSVAEAASDRAAGFVSRRQGEPACRASPADQGQG
                     PELRAWPGEVVGLAGLAGHGQTDMLVALFEQLGPKRSALVAGDRQTDGIFPLWSIAAN
                     ISVGSLRDMVHGLLIDPGAEVELAEEWRRRIGIRTPDMANNILSLSGGNQQKALFARA
                     LGSTADIVLMDDPMRGVDVGTKQEVYAILRAEAARGRTFVWYTTEMDELAHCDHVYVF
                     RDGRITAELAADALTEEKVLHASFADEAA"
     gene            complement(3115..4134)
                     /locus_tag="P5558_09355"
     CDS             complement(3115..4134)
                     /locus_tag="P5558_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24556.1"
                     /translation="MKRTLQGASLAVLALALAGGTAIADTSGKRIALSNNYAGNSWRQ
                     AMLRSWDKVTSQAVADGVVAAADAFTTAENQVTEQAAMIQNLVLQGYDAIVLNAASAD
                     ALNGAVREACDAGVVVVSFDGTVTEPCAWRVTYDYHSMGTAQIDFLAEHMPQGGKLLE
                     IRGLAGVIVDDDISRGIHDGVAANPQFEIVGTVHGDWDQAVAQRAVAGILPSLPAIDA
                     VVTQGGDGYGAAQAFAAAERPTPLIIMGNRQDELVWWKEQKDKNGYTTMSISSAPGVG
                     TLAFWVAQQILDGQDVPKDIIVPLLSISQDELESFLETTEPGGVANVEYTLEDTQGVI
                     AKAAP"
     gene            complement(4268..4750)
                     /locus_tag="P5558_09360"
     CDS             complement(4268..4750)
                     /locus_tag="P5558_09360"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HRY24557.1"
                     /translation="MSEAKLREELYGSFRNRGMIYYNMYKTLSAELGKEKAADLMQDA
                     IYNRGVEQSKVLAGLDNKNVKALMEKFLSIIPDGGKMFGPEILNVEDDAVDIKFHRCP
                     LKECWLEAGIPEDEVAELCRIAARVDNGLFEGAGFKFHAETWEPGRDGCCHLHLRPGD
                     "
     gene            complement(4787..5605)
                     /locus_tag="P5558_09365"
     CDS             complement(4787..5605)
                     /locus_tag="P5558_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24558.1"
                     /translation="MSSTAMHQIDEWAVTDIEPDRRDANETPRADDVVVIEGVDIYYG
                     AVQALFAVDLRVGAGETVAVLGGNASGKSTMIKTALGMVQPRTGAVRVLGRPIAGLEP
                     YRVIGLGVGSIPEGRRVFAEMSVRENLLMGAFVRRQEAKATLAREIDEAIELFPVLQG
                     RMSQLAGTLSGGEQQMLAMARAWMRKPRLLCIDEPSMGLSPLYVDRVYEAIAALKARG
                     ATILMVEQSANRALEVADRAYVLRSGHVVVRGRAAALAGDPAIRRAYLGERAIA"
     gene            complement(5602..>5902)
                     /locus_tag="P5558_09370"
     CDS             complement(5602..>5902)
                     /locus_tag="P5558_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24559.1"
                     /translation="GEPSLLLLDEPTAGMNPTETTELAELIGRITEERPSMSTILVEH
                     KLDVVRNLARRVVVMDNGQVLVEGPPDEVLADLRVVEAYLGKRGAEEARAMGLAP"
     assembly_gap    5903..6002
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6003..7822)
                     /locus_tag="P5558_09375"
     CDS             complement(<6003..7822)
                     /locus_tag="P5558_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24560.1"
                     /translation="MTDLLSQILNGLAIGNVYAMLAIGFVLVFGVANLINFAQGSLFV
                     VGGYLAYTFAVLLGLPLALAASLAVALTALLGLGIDRAFLRPLERGPRIAPLLATLAI
                     AVILDETVQLVWSPDTKPFPSPLASTTWQLGNAYISAVDLLILGTGIATIATLTLFLS
                     RTWIGRAVRATAMDPEAAQQMGVNVAAMRSLTFGIAGLVGGIGGVLVGFYYQSIWPTM
                     GIPLGIKGFTAALLGGIGSIPGAIVGGLLLGVFEALASGYLGSIYRNLIAYVLLLLVL
                     FLRPQGLFGSRGLDALGGAQAAGGAVPTTSLMASPFQTAEPPAITTPWWLAALLLAAA
                     ATFPLWAPDSTWVHILGLALVYAILAVSLTVVAGTAGQVSVGHAGIWGVGAYVAAILT
                     TRYWPGMPIELTLLLAGSAGALAGVLAGFFAARLSGHVIVMATLAAGLILYSVMLVWI
                     PVTRGPMGVFAIPPPVLGILGGVELWRTEQLYWLALAVLALVLLVASRVIGSDVGRLW
                     RAVREDRLAAVASGVPAIRYVLMAFAFSGFLAGVSGALYAHFVTAISPGSFLVLVSFQ
                     ILAIVVLGGLGNLTGAVLGAIVLIAVTEVLRGFGDYRMVT"
     gene            complement(7890..9017)
                     /locus_tag="P5558_09380"
     CDS             complement(7890..9017)
                     /locus_tag="P5558_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24561.1"
                     /translation="MRMKTLALALAAGLAAVASTSVAQEPIVIGLSAPLTGDYAEYGN
                     NFKRAIDLHVEQINAAGGVAGRPLEIVESDDRSDSAQTTAIAQRYVNDPEILAVIGSF
                     ASTPSMAAAPIYQRAGVVQLCPSCSHPDYTKIGEFMFRNTTTQQIEAPAIADFVVEDL
                     GAKTIMVVHRQDDWGLSASGNFIARAEELGAEIVANEAFLETERDFRPVITQLRSLDP
                     DVLHVAMFFTEAAILAQQLQAAGIDIPVVTTTSMFNPQYIVLGGEAVEGHIVPATFFP
                     GNPDPNVQAFVEEYEGRYDIAADSFAAIAYDSVGILAAAIEHVAGQGQEVTRAAIQKA
                     MFEMEPYAGVSGVAAFNEVGDVVKEITWLTIENGEFTVLER"
     gene            complement(9159..9884)
                     /locus_tag="P5558_09385"
     CDS             complement(9159..9884)
                     /locus_tag="P5558_09385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24562.1"
                     /translation="MVELAGQVAIVTGATSGIGMALARALDARGVRLVLTGRRADRLD
                     ALVAELGAARAVAGDITDPVLPGRLVEAALEAHGRLDIACNNAGLLAFGTIEEIDLER
                     MATMVRVNVEATYRFAYTVLRHFKAQNHGHLVNTSSVAGTKVRPTIGAYAGTKHAIEA
                     LAHGLRMELATTDVRISNVQPGLVATDIFAHMPADEHPGRQQGIDQPLRPEDVARAVL
                     FMLEQPAHVSIPTLMLKPTHQPM"
     gene            10047..11513
                     /locus_tag="P5558_09390"
     CDS             10047..11513
                     /locus_tag="P5558_09390"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY24563.1"
                     /translation="MSTTTAEADDDTEPPVVRRSRSSRREQRRRRSSTNGLPRPLLYA
                     TVALLALVPLPIGGQPDWAASLAACLVAGLLLGWCIIAPGRSLQPHVPAVLVVAALLI
                     AGVSVWSLLQVTPGFLPEAWSHPIWARAAAAGLDVEGVVSLNRSASGEALMRLLAACG
                     MFLLAFVLARREADARLLLACILAIITGYAAFGLVDQLLAWRLAEGVGFRSTVSSTFI
                     NRNHFATYANLGLVTALGFILEPLLRGRSEAAEEKLGRRIAAAITLVLEERRYPLMAA
                     VILLLASVGSNSRGGFLSLLGAATFILLLVLLVSRVRPATKLWVILAGLAALGLVIWL
                     TGANLLERFGALELEGGGSRPAAWQMTLDAIRERPLLGYGHGAYVEIFFLRHDVAMGI
                     GVFDHAHNDWLQTAAELGLPAASALWLAQLLLFGLCVHGALARRRRRLYPLVGAGAAC
                     LVALHAVTDFSLLIPAVALTFAALFGIGCAQSTAKSRG"
     gene            complement(11526..12263)
                     /locus_tag="P5558_09395"
     CDS             complement(11526..12263)
                     /locus_tag="P5558_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRY24564.1"
                     /translation="MCNAYSLTHPREALVDLGEALARQGGWRLHADLLPDPLAPRYRV
                     SPRQQAPILRLDRSGRLVWSLGLWGFLVAGGKPGFAPTNARDDRLTSGWPWRQSAETQ
                     RCLIPADGFFEPEKPAGAKGTVPWSYYTLGNRQLFLMAGLWNPAEHPSTGAKVASYTV
                     VTTDASEAIRLHDRMPAILDGDAAALDWLRPGEVPIDRLGPWPAARMRGWRVADAARN
                     SRLPDTPTMIQPVEDAAATSSDQLPHD"
     gene            complement(12315..14414)
                     /locus_tag="P5558_09400"
     CDS             complement(12315..14414)
                     /locus_tag="P5558_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY24565.1"
                     /translation="MAAEDSALSPGRQGAGFFGGARLASLAGRRLALLALLVMAPFTA
                     GLAGTANGLDPFGQAALMRALDATDRGEWSVAREALVELRSPLLFTYVDWRELAESSE
                     PIPFGRYRAFIEGHADWPRLGTIRAQAERRLDAGIDYAGRRAFFADQPPRTREGRIRL
                     AEAELAIGSPERGGELVRRAWVEDGFSASEEAFFLQQFGHLLTRNDHAARLRRLLWDK
                     REDEARRMFRRVDAGLVSLAEARLALQDMEPGVDRRLAAVPRQLARDPGLLYDRLRWR
                     RLKGRDADAREILLDPPDELERPEAWWDERAYQIREALADRDFAGAYRLAKRHGQSPA
                     EAAAFAEAEWLLGWLALRHANEPAQALGRFAQMYDAVRTPISRARAAYWAGRAAAALG
                     DRRATSRWYRLAAEHPTTYYGQLALGELGEPLALAVLAARFATTPVVDPQFDNLPQVQ
                     LVRLFCALEIADRAMPFLQRLVLDQADKSRVMQLAADCGRPDLVVELGKHGVAAGMVD
                     PLVSFPIPAHNGLLYPEDGSVEPALLLAIARQESHFDPKATSSAGAVGLMQVMPGTGK
                     AVAERNAMPWSQRLLQRDPHYNAQIGARYLAMLAERYDGRIELMAAAYNAGPMRVSAW
                     LAANGDPRAADGDAMVDWIELIPFRETRNYVQRVVEGRNVYRDLLTSAAVRPVSFIVD
                     RGPLLPPPVPAAKPRDF"
     gene            14561..15442
                     /gene="dapA"
                     /locus_tag="P5558_09405"
     CDS             14561..15442
                     /gene="dapA"
                     /locus_tag="P5558_09405"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRY24566.1"
                     /translation="MFRGSFVALITPMREGAIDEKAFAGLIEWHIESGTHGFVPVGTT
                     GESPTLDHAEHARVVELCVEIVAGRRPVIAGTGSNATAEAISLTRHAREAGADAALIV
                     TPYYNKPTQAGLFAHYKAIHDAVELPIIIYNIPGRSVVDMTPQTMARLAELPNIIGVK
                     DASNDITRPLATRELCGPDFCQLSGEDGNALAFLAHGGHGCISVTANVAPALCAAMQD
                     AWAAGRSAEALAIQQRLFPLHRDLFLETSPAPAKWALERLGRSGAELRLPLVPIGEAT
                     ARAVEAAMRHAGLLDTA"
     gene            15459..15938
                     /gene="smpB"
                     /locus_tag="P5558_09410"
     CDS             15459..15938
                     /gene="smpB"
                     /locus_tag="P5558_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRY24567.1"
                     /translation="MNERLKARRSVARNRRAFHDYSIEDRIEAGLVLAGTEVKSLREG
                     RATINEAHAGEMGGELWLFNAYIPEYKAGNRFNHETKRPRKLLLRRREIDKLLGAVKR
                     KGMTLVPLSLYFSPRGFAKVELGLARGKKEYDKRHAERDRDWQRDKERLMRHSARDS"
     gene            16041..16919
                     /gene="motA"
                     /locus_tag="P5558_09415"
     CDS             16041..16919
                     /gene="motA"
                     /locus_tag="P5558_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HRY24568.1"
                     /translation="MLAIIGIVLVIGLVFGGYSLAGGKFGIIVKALPFEFMMIGGCAA
                     GTFLVANGMGVAKGALGDLKRVFAGPHWKKDDYRDVLSLMYVIVKLLKTRGVLALEPH
                     VDDPHESSLFSKYPRIQHDHFALDFITDTLRMMTMSMDDPYQVEDCMQRQLKKHHKEL
                     HGRAAALQNVADSLPAIGIVAAVLGIIKTMASIDQPVDVLGGMIGGALVGTFLGVYLA
                     YGFVGPMTARLNQTYDQDGQFYAIIRDCLVAYLQGHSAPIAVELARGNVPTELQPSFQ
                     EIDEVLQALPADPLAA"
     gene            16944..17900
                     /locus_tag="P5558_09420"
     CDS             16944..17900
                     /locus_tag="P5558_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRY24569.1"
                     /translation="MADNSPIVIRKIIEEEGHGHHGGAWKVAYADFVTAMMAFFLMLW
                     LLSTADEQQLKGLAEYFSDAAENKSQPGGMGGVLDGVSFVTPDMAVQAPVSPFAPSSL
                     PLTATPEENDPDDFALDLALAFGLDEAADAAERRPLDDEAFAEEQRRREQAAFDEAKA
                     EMLNALSQRPELARFTDSLIIDDTPEGLRIQIVDREQNAMFPTASAEMLDNARALIGV
                     VAEAIGDLPNRLSIRGHTDARPFASGARYDNWRLSSDRANATREALVEAGLPASRIAD
                     VIGKGPSEPLFPDDPADARNRRISLVLLRDATAPAAGAQGGG"
     gene            complement(17922..19082)
                     /locus_tag="P5558_09425"
     CDS             complement(17922..19082)
                     /locus_tag="P5558_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY24570.1"
                     /translation="MGQTGIGRTAPDASRGIGCGVIGTGYMGACHAQALARVAHTFEP
                     ALRPELVAVADAAIAGAAAARDRFGFARATADWRELLADPKIELVSIATPNVLHKEMA
                     LAALAAGKHVWCEKPLALTAADARELAEAAASAGTTTLVGYNYLRSPAVAFARKLVEA
                     GELGAVTQFRAIFDEDFMGPASFPFSWRCETALAGTGALGDMGSHLVNLAHFLVGPIA
                     EVSAGLATAITERRDAQGNLREVENDDVSETLVRFANGALGHLGCSRIAWGRKNGLDF
                     ELYGRDGGLRFTQERFNEIQLFKPADRADDNGFRTILTGPPHPPFARFTPGWGHGLGF
                     NDLKVIEAAHLLQAIAEETPAWPDFAEGWRVEAVVEAIARSAATRRWEPVPV"
     gene            19315..>19494
                     /locus_tag="P5558_09430"
     CDS             19315..>19494
                     /locus_tag="P5558_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24571.1"
                     /translation="MTTTLSKAARRAADMAGDRATLATAALVVAVAVIGLFAGRWDMA
                     VYTLSFWHYLVYALAF"
BASE COUNT         2942 a         6652 c         7029 g         2771 t
ORIGIN      
        1 tgagcgaggc gccgcccgag aaggccgaga ggagatagcc gcagagcgag gcgaaaagcg
       61 cgctcatcac gtaggtggcg aggcgggtgc ggtcgacggc gatgccggcg agccgggctg
      121 cccgctggtt ctggccgatg gcgatgaccg agcggccgta gatggtgcga tggagcacga
      181 ccgacatgag ggccgccagg acgatgacgc agatggcgag caccggcatg ccgaggaccc
      241 gggcggtggt gaactcggcg agcgccggcg gcggcttgat gcgcaggccg cggccatagg
      301 cgatggcggt cgactggatg atgaagctcg acgagagggt ggcgatgatg ggcgggatgc
      361 gcagggcccg gatcagcgcg tagttgaaga cgccgacggc gaggccgcag cccatggcgg
      421 cgacgaagcc gagcgcgatc agcgcatcct ggccgtccat cagcttggtc gcgacggagc
      481 cggcgagcgt gatggtcgcc gggatcgaga ggtcgacatt gccggggccg agggtgatga
      541 cgaacatctg gccgatgccg acgatgacga agaaggtggc gaaggtgaag gcggcggtca
      601 gcacgtcgcc ggcgccgcgc ccgggcgtga aggggatggt cgcgagccag accagggcgg
      661 ccccgacgaa ggcccagagc cagggcttct cgagcagccg ggcgaggggg cgggacaggg
      721 cgctcactgc cgctcctccg cccggttgat cagggcccga agcgccagga cgaggatgag
      781 gatcgccccg gtggcgccga tctgccagtc cggcgagatc ctgaggaagg agaggaagga
      841 gccggcgagg gtgagcgtga tggcgccgat gacggcaccc gcgggcgaga cccggccgcc
      901 gacgaactcg cagccgccga ggatcgcgcc ggcgatggag aggagggtgt agcgggcggc
      961 gatattggcg tcgcccgagg tggtgaggcc gatcagcgcc atgccggcga gcatgccgaa
     1021 gaccccggcg agggcgaaca tcacgacctt gatcttgagg agcgaccagc cggcccgctc
     1081 gatggcgcgc gggttgccgc cggcgccgcg caggatcgag ccgtaggccg tgcgcatgag
     1141 cgccacatgg acgacgatgg cgatgacggt ggcggcgatg atggggaagg ggacgaccgg
     1201 cgtgtgcagc ttcatcagcc agcggatcca ttcgggcgag gtgccgcccg gccggggcag
     1261 gatgaggacg gcgacgccga gccagacgaa gctcatgccg agggtgacga cgatggatgg
     1321 caggttgcgc agatggatga gcgcaccgag cccggcatag acgaggatgc cggccatgag
     1381 ggccgcgatg ccgaggggcg ggctctcgac cagccaggtg gcggtgacgc aggcgacgta
     1441 gccgacatag gcgccgatcg agaggtcgag ctcgttgacg cagagcacga acatctgggc
     1501 gatggtggcg agcgctatcg ggatcgccag gttgagcatc aaattgaggc cggtgtagct
     1561 catggtcctg ggctgcagcc agaagatggc ggcgagcagc aaggcgagcg acacggccgg
     1621 caggatggcg cggagcgtgc ggggggcgag gagggtcgag gtcacgccgc ctcgtccgcg
     1681 aacgaggcgt gcaggacctt ctcctcggtg agcgcgtcgg cggcgagctc ggcggtgatc
     1741 cggccgtcgc ggaagacata gacgtgatcg cagtgggcga gctcgtccat ctcggtcgtg
     1801 taccagacga aggtcctgcc cctggccgcc tcggcgcgga ggatggcgta gacctcctgc
     1861 ttggtgccga catcgacgcc acgcatcggg tcgtccatca ggacgatgtc ggcggtcgag
     1921 ccgagggcgc gggcgaagag cgccttctgc tggttgccgc ccgagagcga gaggatgttg
     1981 ttggccatgt cgggggtgcg gatgccgatc cgccgccgcc attcctcggc gagctccacc
     2041 tccgcacccg gatcgatcag gaggccatgg accatgtcgc ggagcgagcc gaccgagatg
     2101 ttggcggcga tcgaccagag cgggaagatg ccgtctgtct gccggtcgcc ggcgaccagg
     2161 gccgagcgct tcgggccgag ctgctcgaag agggcgacca gcatgtcggt ctggccgtgc
     2221 ccggcgagcc cggcgaggcc gaccacctcc cccggccagg cccggagctc ggggccctgg
     2281 ccctgatccg ccgggctcgc ccggcaggcg ggctcgccct gccgcctgga gacgaagccg
     2341 gcggcacggt ccgaggccgc ctcggcgacg ctgcccatgg cggcgaccag ggactcccgg
     2401 ctgaaatcgg cggcggcgcg cgcctcgacg accaggccgt cgcgcatcac cacgacgcgg
     2461 tcggacgtct cgaggatctc gccgagcaga tgcgagatca ggatggcgct gccgccctcc
     2521 gcgacgaagc gacggacgaa ggcgaggagc tgctcggcca cgagcgcgtc gagcgaggag
     2581 gtcggctcgt ccaggatgac gaggcgcacc ggcacgtcgg tgaccgagaa ggcgcgggcg
     2641 atctcgacca tctgccggcg ggcgatcgag aggtcgccga ccacctcgcc gggctcgatg
     2701 ccgtgccgcg ggaagatccg gtcgagcccg gccacgacca gctcggcggc acgccgccgc
     2761 cagcccatgc cccggatcga ggggtggaag acccgcgcgt tctcggccac gctcaggttc
     2821 gggcagagcg acagctcctg gaagacgcag cgtatgccgt ggcgctgcgc cgtcaccacg
     2881 tcgtagcccc ggctcagatc ctggccggcg accccgatcc gcccccggtc gggggcgagg
     2941 gtgccggcca ggatctgcat cagcgtcgac ttgccggcgc cgttgtggcc gaccaggccg
     3001 aggcaggtgc cggcgtcgac cacgaggccg acgccggcga gggcacggac ggcgccgaag
     3061 cttttcgcga cgccgtccag ctcgacgatc ggcgccgtca caggccgggg aagctcaagg
     3121 ggcagccttg gcgatcacgc cctgcgtgtc ctcgagggtg tactcgacat tggcgacgcc
     3181 gccgggctcc gttgtctcga ggaaggattc gagctcgtcc tgcgagatgc tcaggagcgg
     3241 cacgatgatg tccttgggca cgtcctggcc gtccaggatc tgctgggcca cccagaaggc
     3301 cagcgtgccg acgccgggcg cgctcgagat ggacatggtc gtgtagccgt tcttgtcctt
     3361 ctgctccttc caccagacga gctcgtcctg ccggttgccc atgatgatca ggggcgtggg
     3421 ccgctcggcc gcggcgaagg cctgggcggc gccgtagccg tctcccccct gggtgacgac
     3481 ggcgtcgatc gccggcaggc tcggcaggat gccggcgacc gcccgctgcg ccacggcctg
     3541 gtcccagtcg ccatgcacgg tgccgacgat ctcgaactgc gggttcgccg ccacgccgtc
     3601 atggatgccg cgcgagatgt cgtcgtcgac gatgacgccg gcgaggccgc ggatctcgag
     3661 gagcttgccg ccttgcggca tgtgctcggc aaggaagtcg atctgcgccg tgcccatgct
     3721 gtgatagtcg tacgtgaccc gccaggcgca gggctcggtg acggtgccgt cgaacgagac
     3781 gacgacgacg ccggcgtcgc aggcctcgcg cacggcaccg ttgagggcgt cggccgaggc
     3841 ggcgttgagc acgatggcgt cgtagccctg gagcaccagg ttctggatca tggccgcctg
     3901 ctcggtgacc tggttctcgg ccgtggtgaa ggcgtccgcg gcggcgacca cgccgtcggc
     3961 caccgcctgg ctcgtcacct tgtcccagct cctgagcatc gcctgccgcc aggaattgcc
     4021 ggcgtagttg ttggagagcg cgatgcgctt gcccgaggtg tcggcgatgg cggtgccacc
     4081 cgccagggcg agggcgagca ccgccagcga ggcgccctgc agcgtgcgtt tcatcgttgt
     4141 tgtcctccca gaggttgttc ttgcgcgaaa ccgggtcgaa aaaccgtccg acgcttcagg
     4201 ctcctagccg gaacgtcgat gccgggaaag ccccgagcgc gccggacggc tggcgttcgt
     4261 gccccgttca gtcgcccggg cgcagatgca ggtggcagca gccgtcgcgg ccgggctccc
     4321 aggtctcggc gtggaacttg aagccggcgc cctcgaagag accgttgtcg acgcgggcgg
     4381 cgatccggca gagctccgcc acctcgtcct cggggatgcc ggcctcgagc cagcattcct
     4441 tcaagggaca gcggtggaac ttgatgtcga ccgcgtcgtc ctcgacgttg aggatctcgg
     4501 ggccgaacat cttgccgcca tcggggatga tggagaggaa cttctccatc agggccttga
     4561 cgttcttgtt gtcgaggccg gccagcacct tcgactgctc gacgccgcgg ttgtagatgg
     4621 cgtcctgcat gaggtccgcc gccttctcct tgccgagctc ggccgagagc gtcttgtaca
     4681 tgttgtagta gatcattccg cgattcctga acgagccgta gagctcctcc ctgagcttcg
     4741 cttccgacat gactggtctc ccttcctctg tgttgactcg atgggctcac gctatcgcgc
     4801 gctcgccgag ataggcgcgg cggatggccg gatcgccggc gagggccgcc gcccggccgc
     4861 gcacgacgac atggccggac cttagcacat aggcgcggtc ggcgacctcc agggcccggt
     4921 tggcgctctg ctcgaccatg aggatcgtgg cgccccgcgc cttcagcgcg gcgatcgcct
     4981 cgtacacccg atcgacatag agcggcgaga gccccatcga cggctcgtcg atgcagagca
     5041 gccggggctt gcgcatccag gcccgggcca tggccagcat ctgctgctcg ccgcccgaga
     5101 gcgtgccggc gagctggctc attcgtccct ggagcaccgg gaagagctcg atcgcctcgt
     5161 cgatctcgcg ggccagcgtc gcctttgcct cctggcggcg gacgaaggcg cccatcagga
     5221 ggttctcgcg cacgctcatc tcggcgaaga cccgccggcc ctcggggatc gagccgacgc
     5281 cgaggccgat cacccgatag ggctcgaggc cggcgatggg ccggccgagc acgcggacgg
     5341 cgcccgtgcg cggctgcacc atgccgagcg ccgtcttgat catcgtcgac ttgcctgacg
     5401 cattgccgcc gaggaccgcc acggtctcgc cggcgccgac gcgaaggtcg acggcgaaga
     5461 gcgcctggac ggccccgtag tagatgtcga cgccctcgat cacgacgacg tcgtcggcgc
     5521 gcggcgtctc gttcgcgtcg cgccggtcgg gctcgatgtc ggtcaccgcc cactcgtcga
     5581 tctggtgcat ggcggtcgag ctcatggcgc gagccccatc gctcgggcct cctccgcacc
     5641 gcgcttgccg agataggcct cgaccacccg aagatcggcc aggacctcgt cgggcgggcc
     5701 ctcgaccagc acctggccat tgtccatcac cacgacccgg cgcgcgaggt tgcggaccac
     5761 atcgagcttg tgctcgacca ggatggtcga catcgagggc cgttcctcgg tgatgcggcc
     5821 gatcagctcg gcgagctcgg tcgtctcggt cgggttcatc ccggccgtcg gctcgtcgag
     5881 cagcaggagc gagggctcgc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nntaggtcac catccgatag tcgccgaagc ccctgagcac ctcggtgacg gcgatcagca
     6061 cgatggcgcc gagcacggcg ccggtgaggt tgccgagccc gcccaggacg acgatcgcga
     6121 ggatctggaa ggagacgagg acgaggaacg agcccggcga gatggcggtg acgaagtgcg
     6181 catagagggc acccgagacg ccggcgagaa agccgctgaa ggcgaaggcc atcaggacgt
     6241 agcggatggc cggcaccccc gaggcgacgg cggcgaggcg atcctcgcgc accgcccgcc
     6301 agagccggcc gacatcggag ccgatcaccc ggctggccac gaggaggacg agagcgagga
     6361 cggccagcgc cagccagtag agctgctccg tccgccagag ctcgacgccg ccgaggatgc
     6421 cgagcacggg tggtggaatg gcgaacaccc ccatgggtcc ccgggtcacc gggatccaga
     6481 ccagcatgac gctgtagagg atgagcccgg ccgccagggt cgccatgacg atcacgtggc
     6541 ccgagagccg ggcggcgaag aagccggcga gcacgccggc cagggccccc gcgctgccgg
     6601 cgagcaggag ggtgagctcg atcggcatgc ccggccagta gcgggtcgtc aggatcgccg
     6661 cgacataggc gccgaccccc cagatcccgg catggccgac cgagacctgg cccgcggtgc
     6721 cggcaacgac ggtgaggctg acggcgagga tggcatagac gagggcgagg ccgaggatgt
     6781 ggacccaggt ggaatccggc gcccagaggg gaaaggtcgc ggcagccgcg aggaggaggg
     6841 cggcgagcca ccagggggtg gtgatcgccg gcggctcggc cgtctggaag gggctcgcca
     6901 tgagcgaggt cgtgggcacg gccccgccgg ccgcctgcgc cccacccagg gcgtcgagcc
     6961 cccggctgcc gaacaggccc tgggggcgca ggaagagcac gagcagcagc agcacgtagg
     7021 cgatgaggtt gcggtagatc gagcccaggt agccgctcgc cagcgcctcg aagacgccga
     7081 gcaggaggcc gccgacgatc gcccccggga tcgagccgat gccgccgagc agcgccgcgg
     7141 tgaagccctt gatgccgagc gggatgccca tggtgggcca gatcgactgg tagtagaagc
     7201 cgaccagcac gccgccgatc ccgcccacca ggccggcgat gccgaaggtc aggctgcgca
     7261 tcgccgcgac gttcacgccc atctgctgcg ccgcctcggg atccatggcg gtggcgcgaa
     7321 cggcccggcc gatccaggtg cggctcaaga agagcgtgag cgtggcgatg gtggcgatgc
     7381 cggtgccgag gatcaggagg tcgacggcgg agatgtaggc gttgccgagc tgccaggtgg
     7441 tcgaggccag ggggctcggg aagggcttgg tatcgggcga ccagacgagc tgcaccgtct
     7501 cgtcgaggat gacggcgatg gcgagcgtgg cgaggagggg ggcgatgcgc ggcccccgct
     7561 cgagcggccg caggaaggcc cggtcgatgc cgaggccgag gagggcggtc agcgccaccg
     7621 ccaggctggc cgccagggcc agcggcaggc cgagcagcac ggcgaaggtg taggccagat
     7681 agccgccgac gacgaagagc gagccttgcg cgaagttgat gaggttggcg accccgaaaa
     7741 ccaggacgaa gccgatcgcc agcatggcgt agacgttgcc gatggcgagg ccgttgagga
     7801 tctggctgag gaggtcggtc atgcgcgccc cggcccggcg cccgcaggtg ccgggccggc
     7861 ccgggaagga tcgcgatcgc ggccggcgat cagcgctcga ggaccgtgaa ctcgccgttc
     7921 tcgatcgtga gccaggtgat ctccttgacg acgtcaccga cctcgttgaa ggcggcgacg
     7981 ccggacacgc ccgcgtaggg ctccatctcg aacatcgcct tctggatcgc cgcccgcgtg
     8041 acctcctggc cctggccggc gacatgctcg atcgcggccg ccaggatgcc gacgctgtcg
     8101 taggcgatgg ccgcgaagct gtcggcggcg atgtcgtagc ggccctcgta ctcctcgacg
     8161 aaggcctgga cgttcgggtc cgggttgccg gggaagaacg tggccggcac gatatgaccc
     8221 tcgaccgcct cgccgcccag gacgatgtat tgcgggttga acatcgaggt ggtggtgacg
     8281 accgggatgt cgatccccgc cgcctggagc tgctgggcca ggatcgccgc ctcggtgaag
     8341 aacatggcga catgcagcac gtccgggtcg agcgagcgga gctgcgtgat caccgggcgg
     8401 aaatcgcgct cggtctcgag gaaggcctcg ttggcgacga tctcggcacc gagctcctcg
     8461 gcgcgcgcga tgaagttgcc ggaagcggaa aggccccagt cgtcctggcg atggacgacc
     8521 atgatggtct tcgcccccaa atcctcgacc acgaagtcgg cgatcgccgg cgcctcgatc
     8581 tgctgggtgg tggtgttgcg gaacatgaac tccccgatct tcgtgtagtc ggggtgcgag
     8641 caggacgggc agagctggac gacgccggcc cgctggtaga tcggggccgc ggccatcgag
     8701 ggcgtgctgg cgaagctgcc gatgaccgcc aggatctccg ggtcgttgac gtagcgctgg
     8761 gcgatcgccg tggtctgcgc cgaatccgag cggtcgtcgc tctcgacgat ctccagcggg
     8821 cgcccggcaa ccccgcccgc cgcgttgatc tgctcgacat gcaggtcgat ggcgcgcttg
     8881 aagttgttgc cgtactcggc gtagtcgccg gtgagcggcg ccgagaggcc gatgacgatc
     8941 ggctcctggg cgacgctggt cgaagcaacg gcagcgaggc ccgcggcgag cgccagggcg
     9001 agggtcttca ttcgcatctg cgtgtcctct cctttgccgc cccgaatgga cgctcatgca
     9061 tggcgtcgct ggccgggagc ggtgacttcc gccggaagag taggcaggtg ctctgcgagc
     9121 ggcaatgacg tttcgcggtc gatcgacggc ggcacccttc acatcggctg gtgggtgggc
     9181 ttcagcatca gggtcgggat gctgacatgg gccggctgct cgagcatgaa gaggacggcg
     9241 cgggcgacgt cctcgggccg gagcggctgg tcgatgccct gctgccggcc cgggtgctcg
     9301 tcggccggca tgtgggcgaa gatgtcggtg gcgacgaggc cgggctggac gttggagatc
     9361 ctgacgtcgg tcgtcgccag ctccatccgg aggccgtgcg ccagggcctc gatggcgtgc
     9421 ttggtgccgg cataggcgcc gatggtcggg cggaccttgg tgccggcgac gctggacgta
     9481 ttgacgagat gcccgtggtt ctgcgccttg aagtgccgga gcacggtgta ggcgaagcgg
     9541 taggtcgcct cgacattgac ccggaccatg gtggccatgc gctcgaggtc gatctcctcg
     9601 atcgtgccga aggcgaggag gccggcattg ttgcaggcga tgtcgagccg gccatgggcc
     9661 tcgagcgccg cctcgaccag gcggccgggg aggaccgggt cggtgatgtc gccggcgacg
     9721 gcgcgtgcgg cacccagctc ggccacgagc gcgtccaggc gatccgcgcg gcggccggtc
     9781 aggaccagcc tgacgccccg cgcgtcgagg gcccgggcga gcgccatgcc gatccccgat
     9841 gtggcccctg tgacgatcgc cacctgtcct gccagctcga ccatgaaggc ctgctccccc
     9901 ctgttggtgc ctgaaccgat ccggccgcca gcgtagtgcc tgaccgccgt ggcggaaagc
     9961 ggcaggccgc cggcgcacgt gcttgcggtc gggttgctgc ttggccagat tggccgggca
    10021 gcggggtggc tttcggggca gaggtcatgt cgacgacgac agcagaggcc gacgacgaca
    10081 cggagccgcc ggtcgtccgc cggtccagga gcagccgacg cgagcaacgg cgccgccgaa
    10141 gcagcacgaa cgggctgccg cggccgctgc tctatgccac cgtggcgctg ctcgccctgg
    10201 tgcccctgcc gatcggcggc cagcccgact gggcggcctc gctcgccgcg tgcctcgtcg
    10261 ccggccttct gctcggctgg tgcatcatcg cccccggccg ctcgctccag ccgcatgtgc
    10321 cagcggttct ggtggtggcg gcgctgttga tcgccggcgt cagcgtctgg agcctgctgc
    10381 aggtgacacc cggtttcctg cccgaggcct ggagccatcc gatctgggca cgggcggcgg
    10441 ccgccggtct cgacgtcgag ggcgttgtca gcctcaaccg ctcggcctcg ggcgaggccc
    10501 tgatgcgcct gctcgccgcc tgcggcatgt tccttctggc cttcgtcctg gcgcgacgcg
    10561 aggcggacgc ccgccttttg ctcgcttgca tcctcgcgat catcacgggc tacgcggcct
    10621 tcggcctcgt cgaccagctc ctcgcctggc ggctcgccga gggcgtcggc tttcgcagca
    10681 ccgtctcctc gaccttcatc aaccgcaacc acttcgcgac ctacgccaat ctcggtctcg
    10741 tcactgccct cgggttcatc ctggagccgc tcctgcgggg ccgctcggag gccgccgaag
    10801 agaagctcgg gcgacgcatc gccgccgcga tcactttggt gctcgaggag cgtcgctatc
    10861 ccctgatggc ggccgtcatc ctgctgctcg cctcggtcgg cagcaattcg cgcggcggtt
    10921 tcctcagcct tctcggcgcc gccacgttca tccttctcct ggtgctgctg gtcagccggg
    10981 tgcggcccgc caccaagctc tgggtcattc tggccggcct cgccgccctg ggcctcgtca
    11041 tctggctgac cggggcgaac ctgctcgagc gcttcggcgc cctggagctc gaagggggtg
    11101 gcagccgccc ggccgcctgg cagatgaccc tcgatgccat acgcgagcgc ccgctcctgg
    11161 ggtacggaca cggtgcctat gtcgagatct tctttctccg gcacgacgtg gcgatgggca
    11221 tcggcgtctt cgaccatgcc cacaacgact ggctgcagac cgctgccgag ctcgggctgc
    11281 ccgctgcctc ggccctctgg ctcgcccagc tcttgctctt cgggctttgc gtccatggcg
    11341 ccctggcgcg ccggcgccgg cggctctacc ccctggtcgg cgccggtgcc gcctgcctcg
    11401 tggcgctgca cgcggtcacc gatttcagcc tcctgatccc ggccgtggcg ctcaccttcg
    11461 ccgccctctt cggcatcggc tgcgcgcaga gcacggcgaa gagccggggc tgacggaccg
    11521 gccggtcagt cgtgcggcag ctgatcggac gaggtggcgg ccgcgtcctc gaccggctgg
    11581 atcatcgtgg gcgtgtcggg caggcgcgag ttgcgcgccg cgtccgccac tcgccagccc
    11641 cgcatgcgtg ccgctggcca tgggccgagc cgatcgatcg gcacctcgcc gggccggagc
    11701 cagtccagtg ccgccgcgtc gccgtcgagg atcgccggca tgcggtcgtg cagccggatc
    11761 gcctcgctcg cgtcggtcgt gaccaccgtg tagctcgcca ccttcgcacc ggtcgacgga
    11821 tgctcggccg gattccacaa gcccgccatg aggaagagct ggcggttgcc cagggtgtag
    11881 tacgaccagg gcaccgtgcc cttggcccct gccggcttct cgggctcgaa gaacccgtcg
    11941 gccgggatca ggcagcgctg cgtctcggcc gactggcgcc agggccagcc gctggtcagc
    12001 ctgtcgtcgc gcgcattggt cggggcgaag cccggcttgc cgccggccac gagaaaaccc
    12061 cacagcccca acgaccagac gaggcggccg gaccggtcga gcctgaggat cggcgcctgc
    12121 tgccgcgggc tgacgcgata gcggggagcg agcgggtcgg gcagcagatc ggcatgcagc
    12181 cgccagccac cctggcgggc gagcgcctcg ccgaggtcga cgagcgcttc tctcggatga
    12241 gtgagcgaat aggcgttgca catgcggcga gcatgccgtc ggcgggcgcc gaggtcgaga
    12301 ccctgcggcc gcgctcagaa gtcccggggc ttggcggccg gcaccggcgg cggcagcagc
    12361 ggcccgcgat cgacgatgaa gctgaccggc cggacggccg ccgaggtcag caagtcgcga
    12421 tagacattgc ggccctcgac gacccgctgc acgtagttgc gcgtctcgcg gaaggggatg
    12481 agctcgatcc agtcgaccat ggcatcgccg tcggccgcgc gcggatcgcc attcgccgcc
    12541 agccaggccg agacccgcat cgggccggcg ttgtaggcgg ccgccatgag ctcgatccgg
    12601 ccgtcgtagc gctcggcaag catcgccagg tagcgggcgc cgatctgcgc gttgtagtgc
    12661 ggatcacgct gcagcagacg ttgcgaccag ggcatggcgt tgcgctcggc gaccgccttg
    12721 ccggtgcccg gcatcacctg catcagcccg acggcgccgg ccgagctcgt cgccttggga
    12781 tcgaaatggc tctcctgccg ggcgatggcg agcaggagcg ccggctcgac gctgccgtcc
    12841 tcgggataga gcaggccgtt atgcgccggg atcggaaagc tcacgagcgg atcgaccatc
    12901 ccggccgcga cgccgtgctt gccgagctcc acgacgaggt ccggccggcc gcagtccgcc
    12961 gccagctgca tgacccgcga cttgtccgcc tggtcgagca cgagccgctg caggaacggc
    13021 atggcccggt cggcgatctc gagcgcgcag aagaggcgca cgagctggac ctgaggcagg
    13081 ttgtcgaact gcggatccac gaccggcgtg gtggcgaagc gggccgccag gacggcgagc
    13141 gccaagggct cgccgagctc gcccagcgcc agctggccat aataggtcgt cggatgctcg
    13201 gcggcgagcc ggtaccagcg ggacgtggct cgtcggtcgc cgagcgccgc cgccgcgcgg
    13261 ccggcccagt aggcggcgcg ggcccggctg atcggcgtgc gcacggcgtc gtacatctgg
    13321 gcgaagcggc cgagcgcctg ggccggctcg ttcgcgtggc gcagcgccag ccagccgagc
    13381 agccactccg cctcggcgaa ggcggcggcc tcggccggag actggccatg ccgtttcgcc
    13441 agccggtagg cgccggcgaa atcacgatcg gccagggcct cgcgaatctg ataggcgcgc
    13501 tcgtcccacc acgcctccgg ccgctccagc tcgtccggcg gatcgagcag gatctccctg
    13561 gcatcggcat cgcggccctt caggcggcgc cagcgcagcc ggtcgtagag caggcccggg
    13621 tcgcgggcga gctgccgcgg cacggccgcg agacgccgat ccacgcccgg ttccatgtcc
    13681 tgcagggcga ggcgtgcctc ggcgaggctc accagccccg catcgacgcg ccggaacatc
    13741 cgccgggcct cgtcctcgcg cttgtcccac agcagccggc gcagccgcgc cgcgtggtcg
    13801 ttcctggtca ggagatgacc gaactgctgc aggaagaagg cctcctcgct ggcgctgaag
    13861 ccgtcctcca cccaggcccg gcggaccagc tcgccaccgc gttccggact gccgatggcc
    13921 agctccgcct cggccagccg gatccggccc tcgcgggtcc gtggcggctg gtcggcgaag
    13981 aaggcacggc gcccggcata gtcgatgccc gcgtcgaggc ggcgctcggc ctgggcgcgg
    14041 atcgtgccga ggcgcggcca gtcggcgtgg ccctcgatga atgcccggta gcggccgaag
    14101 ggaatcggct cgctgctctc ggcgagctcg cgccagtcga cataggtgaa gaggagcggc
    14161 gagcgcagct cgaccaacgc ctcccgggcg acgctccatt ccccccggtc ggtggcatcg
    14221 agcgcccgca tgagggcggc ttgcccgaag ggatcgagcc cgttcgcggt ccccgccaga
    14281 ccagccgtga acggggccat gacgaggagc gccaggaggg cgaggcggcg gccggcgagc
    14341 gacgccagcc gggcaccgcc gaagaagccg gcgccttgcc ggcccggcga cagcgcacta
    14401 tcctctgccg ccacgccgtt ctccacaagg gccggcggcg tcttcatccg ctcgcccccg
    14461 atgtcgggac cgaccccggc aaaccggctt cgccgttcaa ccggcaaacc ggcttcggag
    14521 tcccgatctc ccgaggcccg atctctcgag gaaagccccg atgtttcgtg gatcgttcgt
    14581 cgccctgatc accccgatgc gcgagggggc catcgacgag aaggcgttcg ccgggctgat
    14641 cgagtggcat atcgaaagcg ggacgcacgg cttcgtgccg gtcggcacga ccggcgagag
    14701 cccgaccctc gaccatgccg agcatgcgcg ggtcgtcgag ctctgcgtcg agatcgtggc
    14761 cggacgccgc ccggtgatcg ccggcaccgg ctcgaacgcg acggccgagg cgatcagcct
    14821 gacccggcac gccagggaag ccggcgccga tgccgcgctg atcgtcacgc cctactacaa
    14881 caagccgacc caggccggtc tcttcgcgca ttacaaggcc atccacgacg ccgtcgagct
    14941 gccgatcatc atctacaaca tccccggccg cagcgtcgtc gacatgacac cgcagaccat
    15001 ggcacgcctc gccgaactcc ctaacatcat aggggttaag gatgcgtcaa atgatattac
    15061 acggccgctc gcgacgcgcg agctttgcgg cccggatttc tgccagctct ccggcgagga
    15121 cggcaatgcc ctggcctttc tggcgcatgg cggccatggc tgcatctcgg tgacggccaa
    15181 tgtggcgccg gccctctgcg ccgccatgca ggatgcctgg gccgccggcc ggagcgccga
    15241 ggcgctggcg atccagcagc ggctttttcc cctgcaccgc gacctcttcc tggagaccag
    15301 cccggcgccg gcgaaatggg cgctcgagcg gctcggccgc tcgggcgccg agctgcgcct
    15361 gccgctcgtc cccatcggcg aggcgaccgc cagggccgtc gaggccgcca tgcgccacgc
    15421 cgggctcctc gacaccgcct gaacctgggg cacgacacat gaacgagcgg ctgaaggcgc
    15481 ggcgcagcgt cgcgcggaac cggcgcgcct tccacgacta cagcatcgag gatcgcatcg
    15541 aggccggcct cgtcctcgcc ggcaccgagg tcaagtcgct gcgcgagggc cgggcgacga
    15601 tcaacgaggc ccatgccggg gagatgggcg gcgagctctg gctcttcaac gcctacatcc
    15661 ccgagtacaa ggcggggaac cgcttcaacc acgagaccaa gcggccgcgc aagctgctgc
    15721 tccggcggcg cgagatcgac aagctcctgg gtgccgtcaa gcgcaagggc atgacgctgg
    15781 tcccgctctc actctatttc tcgccacgcg gcttcgccaa ggtcgagctc ggcctggcgc
    15841 gcggcaagaa ggagtacgac aagcgccatg ccgagcgcga ccgcgactgg cagcgcgaca
    15901 aggagcgcct catgcgccac tcggcccggg acagctaggg gcccgctgga agccaggggg
    15961 cacggttacg aaatcttaag gccgctcggc tagaacccct tgggcacggc tcggaccgag
    16021 ccctgcctgc gggagcggca atgctagcga tcatcggcat cgtcctggtc atcgggctgg
    16081 tcttcggcgg ctactcgctg gccggcggca agttcggcat catcgtcaag gcgctgccgt
    16141 tcgagttcat gatgatcggc ggctgcgccg ccggcacctt cctcgtcgcc aacggcatgg
    16201 gtgtcgccaa gggtgccctc ggtgatctca agcgcgtgtt cgccggcccg cactggaaga
    16261 aggacgacta ccgcgacgtg ctctcgctca tgtacgtcat cgtcaagctt ttgaagacgc
    16321 gcggtgttct cgccctcgag ccgcatgtcg acgacccgca cgagagtagc ctcttctcca
    16381 agtacccgcg catccagcac gaccatttcg ccctcgactt catcaccgac acgctgcgga
    16441 tgatgacgat gagcatggac gatccctacc aggtcgagga ctgcatgcag cggcagctca
    16501 agaagcacca caaggagctg cacggccggg ccgcggcgct gcagaacgtc gccgattccc
    16561 tgccggcgat cggcatcgtc gccgccgtgc tcggcatcat caagaccatg gcgtcgatcg
    16621 accagccggt cgacgtgctg ggcgggatga tcggcggcgc gctcgtcggc acgtttctcg
    16681 gcgtctacct cgcctatggc ttcgtcgggc cgatgacggc ccggctcaac cagacctacg
    16741 accaggacgg ccagttctac gccatcatcc gcgactgcct cgtggcctat ctgcagggtc
    16801 attcggcgcc gatcgcggtc gagctcgccc gcggcaacgt gccgaccgag ctgcagccga
    16861 gcttccagga gatcgacgag gtgctgcagg ccctgcccgc cgatccgctc gccgcctgac
    16921 cggcagacgg cagcccctcc gccatggccg acaacagccc gatcgtcatc cgcaagatca
    16981 tcgaggagga ggggcacggc catcacggcg gtgcctggaa ggtcgcctat gccgatttcg
    17041 tgacggcgat gatggccttc ttcctcatgc tctggctgct cagcacggcc gacgagcagc
    17101 agctcaaggg gctcgccgag tatttcagcg atgcggccga gaacaagagc cagccgggcg
    17161 gcatgggtgg cgtgctcgac ggcgtctcct tcgtcacgcc ggacatggcg gtccaggcgc
    17221 cggtctcgcc cttcgccccc tccagcctgc ctttgacggc gacgccggag gagaacgatc
    17281 cggacgattt cgccctcgac ctggcgctcg ccttcggcct cgacgaggcg gcggacgcgg
    17341 ccgaacgccg gccgctcgac gacgaggctt tcgccgagga gcagcggcgc cgcgagcagg
    17401 cggcgttcga cgaggcgaag gcggagatgc tgaacgcgct gagccagcgg ccggagctgg
    17461 cacggttcac cgacagcctg atcatcgacg acacgcccga ggggctgcgc atccagatcg
    17521 tcgaccgcga gcagaacgcc atgttcccga ccgcctcggc cgagatgctc gacaatgccc
    17581 gggccctcat cggcgtcgtc gccgaggcca tcggggatct cccgaaccgg ctctcgatcc
    17641 gcggccacac cgatgcccgg cccttcgcca gcggtgcccg atacgacaac tggcgcctgt
    17701 cgtcggaccg ggcgaacgcc acccgcgagg cgctggtgga agcgggcctg ccggcatcgc
    17761 ggatcgccga cgtcatcggc aagggcccgt ccgagccgct tttcccggac gacccggccg
    17821 atgcgcgcaa ccggcgcatc tcgctcgtcc tgctgcgcga cgcgaccgcc ccagccgccg
    17881 gagcccaggg cggcgggtag gtgccgaagc cgcccggcgg gtcagaccgg cacgggctcc
    17941 cagcgcctgg tggcggcgct tctggcgatc gcctcgacca ccgcctcgac ccgccagccc
    18001 tcggcgaaat cgggccaggc gggtgtttcc tccgcaatgg cctggaggag gtgggccgcc
    18061 tcgatcacct tgaggtcgtt gaagccgagc ccgtggcccc agcccggggt gaagcgggcg
    18121 aagggggggt gcggcggtcc cgtcaggatc gtgcgaaacc cgttgtcgtc ggcgcggtcg
    18181 gccggcttga agagctggat ctcgttgaac cgctcctggg tgaagcggag acccccgtcc
    18241 cggccgtaga gctcgaaatc gaggccgttc ttgcggcccc aggcgatgcg cgagcagccg
    18301 agatggccga gggcgccatt ggcgaagcgg accagggtct cgctgacatc gtcgttctcg
    18361 acctcgcgca ggtttccctg cgcatcgcgg cgctcggtga tcgccgtggc gaggccggcg
    18421 ctgacctcgg cgatcgggcc gaccaggaaa tgggcgagat tgaccagatg gctgcccatg
    18481 tcgccgagcg ccccggtgcc cgcgagcgcc gtctcgcagc gccaggagaa ggggaagctc
    18541 gccggcccca tgaagtcctc gtcgaagatg gcgcgaaact gcgtcacggc accgagctcg
    18601 ccggcctcga ccagcttcct cgcgaaggcc acggcgggcg agcggagata gttgtagccg
    18661 acgagcgtgg tcgtgccggc cgacgctgcc gcctcggcca gctccctggc gtcggccgcg
    18721 gtcagcgcca gcggcttctc acaccagaca tgcttgccgg cggcgagggc ggccagcgcc
    18781 atctccttgt gcagcacgtt gggggtcgcg atggagacca gctcgatttt cgggtcggcc
    18841 aggagctccc gccagtcggc ggtcgcccgg gcgaagccga agcggtcccg ggcggccgcc
    18901 gcgccggcga tggcggcatc ggcgacggcc accagctcgg gccgcagcgc cggctcgaag
    18961 gtatgggcca cccgggccag cgcctgggcg tggcaggcgc ccatgtagcc tgtgccgatg
    19021 acgccgcagc cgatgccgcg cgatgcgtcc ggcgcggtcc tgccgatccc cgtctgtccc
    19081 acgatagcct cctctgccat ctgtcggcag ctgctatgcc ccgccgccgc gacgtgtaca
    19141 aggcgaggcg gtgggcggcg gacttgtgct cctcaccggg gcccgccagg gcccctgcat
    19201 tgacgacacg ctgcttgtcg gttgccaccg gcgatcgtag aggaccgagg ccgagcgact
    19261 ctcgcctcga accagcggag cagagcgacg ggcaccgatt gcgggatgag ccatttgacc
    19321 accaccctgt cgaaagcagc gcggcgggcg gccgatatgg cgggcgatcg ggcgaccctc
    19381 gccacggccg ccctcgtcgt cgcggtcgcc gtgatcggcc tctttgccgg acgctgggac
    19441 atggcggtct acaccctcag cttctggcac tatctcgtct acgcgctggc cttc
//
LOCUS       DAOTFI010000074        19424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3377_length_19424_cov_6.882028, whole genome shotgun sequence.
ACCESSION   DAOTFI010000074 DAOTFI010000000
VERSION     DAOTFI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19424
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3377_length_19424_cov_6.882028"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="P5558_09435"
     CDS             <1..227
                     /locus_tag="P5558_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24572.1"
                     /translation="ISAVALGGVSLAGGKGGLLAAALGAIDIFLLQSVLTHFNVSTFL
                     LQMAYGAALTIAVALNSSRLGALLFRGRRA"
     gene            224..1180
                     /locus_tag="P5558_09440"
     CDS             224..1180
                     /locus_tag="P5558_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24573.1"
                     /translation="MRGHFRISGPLATLLLVLVVHLVGMLLIKGFGTTFGVRAMLVLA
                     ALLAIASIGQTLVILLGGIDLSIPFVIGFANVAAAQLYGQGVPFAVVLPGVILAAMLV
                     GAFNGALSSILAIHPLIVTLGTGTTLQGAVQLWTKGFPTGGAPAWVQKFVSIGGTIGP
                     VPVPWLIPFTGVLALGVVLLLSRTVYGRRLYALGSNPRAARLALVDPVRMWTVTFALS
                     AGFAALAGILLLGFSGSAYAAVGAPYLFQTIAAVVIGGTALVGGRGGYLGTLAGALCL
                     IELTTVLVGLGLQPPLVQATLGVLIVALVAVYGRESHVRELI"
     gene            complement(1185..2309)
                     /locus_tag="P5558_09445"
     CDS             complement(1185..2309)
                     /locus_tag="P5558_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24574.1"
                     /translation="MISGTRILAIMIKELLQLSRDRLTYAMILVMPVMQLMLFGYAIN
                     TDPRHLPTVVLDEDRTTLSRSVLAALGNTGYFDFTAEAASHAAIDAAIESGAAQLAVV
                     IPHDFSRRVVKGDAPRILVEADASDPTAVGSALGSLGGVAEAALDHDLQGPLARDGGG
                     GPFEIVVHRRYNPENITAFNIVPGLLGIVLSMTLVMMTALAVTREHERGTMESLLATP
                     VRPIEVMIGKLAPYVGIGVIQTVLILGLARLLFGVPFEGGWAALALGTALFITGSLAL
                     GFLFSTLARSQLQAMQMSIFYILPSILLSGFMFPFRGMPDWAQVLGTAIPVTHFLRVV
                     RGSLLKGADLAQTWPSLAALGVFVVVVTALAMARYRTTLD"
     gene            complement(2306..3223)
                     /locus_tag="P5558_09450"
     CDS             complement(2306..3223)
                     /locus_tag="P5558_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24575.1"
                     /translation="MPVAIDVKGLNKSFGDKHVVRDVSITVEAGRVTGFLGPNGSGKT
                     TTMRMLCGLLTPDSGEGTCLGLDILKETGEIKRRTGYMTQRFSLYDDLTIEENLLFVA
                     RVYGMDDRRRRVAEALAGLGLEARRKQPAGALSGGWKQRLALAAAVMHEPRLLLLDEP
                     TAGVDPKARREFWDQIHELTAKGMTVMVSTHYMDEAERCHDIAYIAYGALIARGTVAE
                     LIDRSALVTFKGEGPGADRLAPALLREPGVEMAAPFGAALHVSGPDRALLERAISRHR
                     GDGLRWTEGRPTIEDVFIHLMRGAEDNFR"
     gene            complement(3227..4207)
                     /locus_tag="P5558_09455"
     CDS             complement(3227..4207)
                     /locus_tag="P5558_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRY24576.1"
                     /translation="MKRRILRVLLLLVVIALTVAGGHWWLENRVTTPPLLTGYVEGEA
                     LYLAGPMAAELARLDVDRGDRVAAGQSLFVIDQRAAEATLEGARAAAAAAQARVEDAR
                     KGMRAEEMAVIEAERDAAKAQLEEAKLELDRVRPLVERGATSRARLDEALANYRTADA
                     QLAQVMRQLDAAQLGARADLVAAAEAEAERAQAAVDEAEVLLAQLAVAAPAAGLIENV
                     FFRAGEWVPASQPVLSLLPDERVKIRFFVPQAAVATYRTGGEVTFTCDGCGAPRAARI
                     TFVSPRVEYTPPVIYSQESREKLVFLIEAEPVDPAGLAPGQPVDVTPLGP"
     gene            complement(4204..4839)
                     /locus_tag="P5558_09460"
     CDS             complement(4204..4839)
                     /locus_tag="P5558_09460"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY24577.1"
                     /translation="MTTAKFRRRKEARPGEIVEAALALFSEKGFAATRLDEIAARAGV
                     SKGALYLYFATKEELFRAVVTQAVAADLERVGAFARAHQGTFAELLSTVVRLLARTAA
                     TGSIGPMAKAVISESRNFPELARHWHDAVVAPMLSLMAAAIARAQAKGEVRPGDPRLY
                     AMQVASPLIMGMLWRETFVPGGAEPFDLGELAEQHVRTLLHGLAERPDGAP"
     gene            4970..5356
                     /locus_tag="P5558_09465"
     CDS             4970..5356
                     /locus_tag="P5558_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HRY24578.1"
                     /translation="MNDNDAAKAIAAVNGRIVAAFKDRDAAAAAACYTADGRLMAPGA
                     EPHVGPAAIQASIQSGFAAGVGGLRLETVALEVLGDTAWEEGIYEVFDAAGSSRDLGK
                     YIVIWKRVGGAWLIARDIMSTNRAAA"
     gene            5424..6191
                     /locus_tag="P5558_09470"
     CDS             5424..6191
                     /locus_tag="P5558_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24579.1"
                     /translation="MTALLEGRKAVITGAGTGIGRAIALALVREGATVAVTDLDACQA
                     ARVAAEAGARAFALDVTDAAATSRVLDEAAAAMGGFDLLCANAGISTMRAVVDLSEDE
                     WDANMAVNAKGVFLSNQAACRRWLADGTKGVIVNTASLAAKQGAPLLAHYSASKFAVM
                     GFTQALAREVAPQIRVNCVCPGFVRTGMQHREVEWEAELRGMSEEAVRDEYVSLTPLG
                     RLEEPEDVADAIVFLASDLARFITGESLNVTGGVRTD"
     gene            6195..7013
                     /locus_tag="P5558_09475"
     CDS             6195..7013
                     /locus_tag="P5558_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY24580.1"
                     /translation="MLVRRAAATILPHVVLVVFSAAILLPLLWVLRVSLTDKLTAYRI
                     PPEWTEPRLDNYIEIFTAYRFADWFSNSLIVALASTLLALPLAVAMAYAFARHDTGGA
                     PLRLGVLASQMLPPIVLVVPLFALFLGAGLLNTYAALVIAHLTFNIPFLAWMLVAFFE
                     GEVRQLEEAARIEGATRFQAFIRVSVPLAAPGIMAAGLLGFILSWNEFLLALILSGRA
                     TQTLPVGLSSLETHRGVEIALLAAAALLAILPVFVLLPFLRKYLIKGLSLGALK"
     gene            7067..8341
                     /locus_tag="P5558_09480"
     CDS             7067..8341
                     /locus_tag="P5558_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY24581.1"
                     /translation="MRKLLLGTTILGLGFGAAEAQTLNILMESVPDTRFVEELLPEFE
                     AATGIDVELEVVNYAEMHTKLVPQLVSPTGSYDVIVVDFYWVGEFAKAGWLQPLDERI
                     AADGFDTSVYFDSLQDLVGQVDGEQLMLPFYNYAMGLTYRHDLLEDPAEQAAFKEKYG
                     IELRVPETWDEYLQQVEFFTRDTDGDGKNDFFGVVNQGLRPDPIAMEWSNYLFANGGR
                     FHDESWQPMLDSPEAVKAVEDYKRNLEEFGPLGAASFGFDEAFNVAAQGKAYSYLTYN
                     MFRTAYDDPSQSAVVDKMEIVAVPNGGLNGAWGWAIPNSSPDPDAAWTFLKWVESPEI
                     AKKRAMLGGSPTRKDVFADEEVLAKYPYYPALENLLATSHNFPVFTYTPQFVEVLGRE
                     LSLAVTGEKTAEEAMQTANAELEALLKQDGKL"
     gene            8363..9241
                     /locus_tag="P5558_09485"
     CDS             8363..9241
                     /locus_tag="P5558_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY24582.1"
                     /translation="MAATLAARDRRFGQLMAAPGLAALLLVIVFPILFTLYTSAFDFT
                     LINPRHDTFVGFAHYARAFGLAEFGNALLVTLWFVVAVVVLEFAVGFLVALMLNAVER
                     GKAVYYAILLCPLLINPVVVGLIWRMFLHPELGIANYVLGLVGLGPVNWLGDPRNAFW
                     TLVVVDIWHQVSFMIVLLLAGLASLPKEPYEAARMEGAGTLACFWHVTLPLMRPVILV
                     TLLIRLIFAVRTYDLVYIMTRGGPGQATDLVSYFIYRQAFVGLNIGQASALAVLLLLI
                     VLVLTAWLYRHMRSLV"
     gene            9241..10317
                     /gene="ugpC"
                     /locus_tag="P5558_09490"
     CDS             9241..10317
                     /gene="ugpC"
                     /locus_tag="P5558_09490"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY24583.1"
                     /translation="MPDIRLSNVTKVYGGNVVAVDGIDLEIEDGEFLILLGPSGCGKS
                     TTLRMIAGLETITSGDLFVGERRVNEVDPKDRDLAFVFQNYALFPHMTVAGNLAFGMR
                     LRGVPKATIQSRIAEATALLGLDGLLQRKPKELSGGQRQRVALGRALLRDPVAFLLDE
                     PLSNLDAKLRAQMRTELVKLHRRVGRTIIHVTHDQVEAMTMGDRLCIMNGGRIVQVGR
                     PMDVYRNPADTFVAGFLASPPMNLIEGRIENERIVVQGLELPLAGFAPKSADIVLGIR
                     PEDLAAVAMGPTAVPVDLDVVAIEALGPELVVIGGLGAGGAEIAARLSPDFVAAIDAP
                     CRLWLDPARIALFDAATTRAVARS"
     gene            10415..11752
                     /locus_tag="P5558_09495"
     CDS             10415..11752
                     /locus_tag="P5558_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY24584.1"
                     /translation="MSRNRLLASASVVALGLVAAGGAVAQDLKPTSGETVFFRGWQYK
                     TDIVQSNVDRYNSELDGKVDYATVTGDYPAIMETNLIAGAELDILYANPSQAARYYDG
                     GWVLPAESLPNIDEIKADMYPNILDAWTYKGELTGLSYFVSTRGVMHVNLKQYGELGY
                     TDADFPATWDELYDQLYEIRDKGVEQPYLPHWLSEWYGISWGFVFEVMNRGGDFADPE
                     THQPTLDTSEGGPAWATLEDWKRIFNDGLVPEEVLTYNESAYIDAYASGRYVFSPQQL
                     YDLETFNRPDRSQIAGEASLLPYQGQSWGLLDSAVYLMTSRERDEAHTDDVKKFASWY
                     GFKDQNGEIFVGQRWMQESMLFSGYKSVMESEATAERMKGALARPGDVEAVLGVYAET
                     PYPKGIWNVVWSEEFNSWLREELQAFILDDGDIGDMLESINEKITELNAKYGI"
     gene            complement(11781..12539)
                     /locus_tag="P5558_09500"
     CDS             complement(11781..12539)
                     /locus_tag="P5558_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24585.1"
                     /translation="MGYLERFRLDGRRAVVTGAARGIGFASAEALRDAGAALWLVDRE
                     ADLVAAAAGRLDARHRALDLRDAAAVEAMAEAVWAEAGGIDVLVNCVGICRNTPVTEI
                     PDDEWREVIDVNLNAMFWSCRAFGRRMAEAGGGSIVNIGSNSGVIADKPQPQAHYNAS
                     KGAVHMLTKSLAVELASKGVRVNAVAPSYTLTEMTKLGLANSEWARIWAEMTPMGRFA
                     EPEEIASAVLFLASPASSYTTGSILLVDGGYTSW"
     gene            12702..13598
                     /locus_tag="P5558_09505"
     CDS             12702..13598
                     /locus_tag="P5558_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY24586.1"
                     /translation="MTSTGRTSELSNLQFALLMALPVLLFLVLVVAYPLGYALWMSFQ
                     QIVFFGGYRTNFVGLANYATMLGDDKFWWSIWVTVRFTVESVLLAMAIGLALALLLNR
                     PMRRPGLIRTIIMLPWCVSLYGTGIMFSYLATGQTGFATAIAFFFGADRPVSFVNRQW
                     VIEVLAIGNAWNMAPLVAFFLLANLKTIPTRLYDLAAIDRMSPLETFWNVTLPPLRFT
                     LFVFTCITTVLSMKLFDFIFVLSGGGPGTASAVMTYQIYKISFRDLNLGYGAAQSFVL
                     LFLIVGSTMTLYALWGRREAAR"
     gene            13598..14461
                     /locus_tag="P5558_09510"
     CDS             13598..14461
                     /locus_tag="P5558_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY24587.1"
                     /translation="MQRRMTLWAWLGIAVAVVWTLLPIYWFLKMSLLTPDEIARFPPP
                     LVPLDPTPAAYFNIFGFDYQVTPELVRRASGQAGQIINGLINSLIVSIVVTAITMLVV
                     VPLAYVFARLDFRFKQALLITILLSVALPPVSTLIPFYALYVQLGLAGTLFGLIVVTL
                     TITIPFVTWMLIGYLRNLPPVERLARVDGYTRLETLVFIVIPLARSGIAVAAVIAFLF
                     AWNEYTYALVLVNGTPANTLATALSGFLFQHPEPSHLAAALVVSILPPVLVAYFLQRH
                     IAEMNLVDPIR"
     gene            14495..15547
                     /locus_tag="P5558_09515"
     CDS             14495..15547
                     /locus_tag="P5558_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24588.1"
                     /translation="MAGIRLDHLKKSFGAFTAVQDLDLDVTDGEFLVLLGPSGCGKTT
                     TLNCIAGLETPSAGRILFDGRDVTSDPPHSRNIAMVFQSALLYPHMTAAANIEASLRR
                     SDLAPEERRRRIDDAVSMLEIGDLLDKLPSEMSGGQRQRVATAKAIVRQPTAFLLDEP
                     LSALDAALRMTLRAEIVNLQKRLGSTTVFVTHDQVEAMTMGDRIAVMNGGRLEQIGTP
                     SEIYERPRSLFVAGFVGSPPMNLIRGEVADGVFAAGELRVPLAAAPGPVTLGVRPQHL
                     HVRAEGGLPCAIFALERLGRETVLIVEDRARNRLRLLVEAEFEGRVGDPLDVAPDPAR
                     CLLFDDASGLIHAPAN"
     gene            15558..16556
                     /locus_tag="P5558_09520"
     CDS             15558..16556
                     /locus_tag="P5558_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY24589.1"
                     /translation="MAKLRMGVVGLGFMGSLHARLWHELPNTELVAVADLDPATGEPL
                     ARKYGCAFHADPADLFARADIDAVSIVTPDRHHVAPATAAAAAGKHILLEKPMAHTGA
                     AAAEIAAAVEQHGVRLMVAHVLRFDPRYVQLRAAADPETFGEAIHLRAKRNSVRGLAQ
                     RLGANSSVLYYLGVHDVDMLQWVARSPITRVYAQKVQKLSNGNEDALYAVLNFASGAI
                     GTLDYSWAWPDGLPNGYWVHLEAVGTKSAAFLEVRDQGLHLVGETGTVGADTHLWPEI
                     NGKIIGDLRDELLHFAEAVQSGEPFVQDWRDAQDAIRVLDALFASVDQGAPVDVRR"
     gene            complement(16553..17443)
                     /locus_tag="P5558_09525"
     CDS             complement(16553..17443)
                     /locus_tag="P5558_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY24590.1"
                     /translation="MTGRLADRLIVNLAALLVLAFAAGPILLAAFGSVVPDRILFDRD
                     RSVLEGGMTLDNYRYIFTGRLPAAYMAEGANRAMISDAARQVPRSLWNSAQISTSVLA
                     LNLLLGAPAAFAFARFAFPRKKPAFLFLILSPLVPAVALVTPIYLLVDGLDLLGTKLA
                     VVLVHTARSLPFTILILSVFFRKLPDELFEAAVIDGCSRLQSFLRVALPLALPAVCAT
                     GLFAFMLSYTEFLFSMVLSGDARSRPLSVVMAALARNTDVSWSLLNSAIFIAVLPTLL
                     LVVLVWRYVVEGLLAGAVKG"
     gene            complement(17440..18291)
                     /locus_tag="P5558_09530"
     CDS             complement(17440..18291)
                     /locus_tag="P5558_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY24591.1"
                     /translation="MLLVTLFTVVPIGHTLWTSLHSAHFLFPDELSWSGLDNYRKVIA
                     GPFFLTTLKNSLLFTAVTAPVVVGLGIATALLLQRRFTGRAVLRSVVLLPWVLPGAIT
                     AVLWIWVFNPSWGMLNTWLRDLGLITSSIPWLSDPSLAFASVAVAHIWSQVPFSVVLI
                     MAALSTIDPALFEAARIDGANAWQRFRFVVWPAIKAMVVVLLVYNALTAFTTYDVVYA
                     MTGGGPGTATSLLSFQIWRESFSMYDFGAGSAVAFLVVAMAGLMIVAITRALPSDLFA
                     ATEDEAP"
     gene            complement(18383..>19424)
                     /locus_tag="P5558_09535"
     CDS             complement(18383..>19424)
                     /locus_tag="P5558_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY24592.1"
                     /translation="TTTDVIYGGQDWLPAWAAAGYLYPLDEIAADAIESYKKDLAGFT
                     INDMTYDGHLYGLPYYADTISFIYNQRILKDAAIEVPTSWEEVADAARKLKANGMERP
                     IVYEFNQELPNFYDAFVAQAYGRGGDLFDEEGEAIFNRPDNAAFQQLSWLRDVIAEGL
                     VAFENHESTIIPAMNSGKHAFTIVYNYVLAAMNNKADQPLAGEFALARMPGEAQSTLG
                     FTKFYAITSSAAENRERREAAWKFVDFMAGPPYAVAKRWAIEKGLGFGQLPLLEDPEV
                     IEAWSGWVDMPTLQAQIETARGGTWKEWTAVWAASFRPLMAQAMIGEATVEEVMSQGA
                     ERWDALRAQMAR"
BASE COUNT         3010 a         6675 c         6519 g         3220 t
ORIGIN      
        1 ccatctccgc ggtggcgctc ggcggcgtca gcctcgccgg cggcaagggc gggctgctgg
       61 cggcggcgct cggcgccatc gacatcttcc tgctgcaatc ggtgctcacg cacttcaatg
      121 tctcgacctt cctcctgcag atggcctacg gcgcggcgct caccattgcc gtggccttga
      181 actcgtcccg cctcggcgcg ctcctctttc ggggcaggcg ggcatgaggg ggcacttccg
      241 catcagcggt ccgctcgcca cgctgctttt ggtgctggtc gtccatctcg tcggcatgct
      301 cttgatcaag ggcttcggca ccaccttcgg cgtgcgcgcc atgctggtgc tggcggcgct
      361 gctcgccatc gcgtcgatcg gccagacgct ggtcatcctg ctcggcggca tcgatctctc
      421 gatcccgttc gtcatcggct tcgccaatgt cgccgccgcg cagctctacg ggcagggcgt
      481 gcccttcgcc gtcgtcctgc ccggtgtgat cctggccgcc atgctggtgg gtgcgttcaa
      541 cggtgcgctg tcgtcgatcc ttgccatcca cccgctgatc gtgacgctgg gcaccggcac
      601 gacgctgcag ggtgccgtgc agctctggac caagggtttc ccgaccggcg gagcgcccgc
      661 ctgggtgcag aagttcgtct ccataggcgg gaccatcggg ccggtgccgg tgccctggct
      721 gatcccgttc accggagtgc tggcgctggg cgtggtcctc ctcctctcgc gcaccgtcta
      781 tggccggcgg ctctacgcgc tcggcagcaa cccgcgcgcg gcgcgcctgg cgctggtcga
      841 tccggtgcgc atgtggacgg tcaccttcgc gctctccgcc ggcttcgccg ccctcgccgg
      901 catcctgctg ctaggcttct ccggctcggc ctatgcggcg gtgggtgctc cctacctctt
      961 ccagaccatc gccgccgtgg tgatcggcgg cacggccctg gtcggcgggc gcggcggcta
     1021 tctcggcacg ctggccggcg cgctctgcct gatcgagctc accaccgtgc tggtcggcct
     1081 cggcctgcag ccgcctctcg tgcaggcgac gctgggtgtg ctgatcgtcg ccctcgtcgc
     1141 ggtctatggc cgcgagagcc atgtccgcga gctgatctga cgtatcagtc cagcgtggtc
     1201 cggtagcggg ccatggccag cgccgtcacc acgaccacga agaccccgag ggcggcgagg
     1261 ctcggccagg tctgggcgag atcggcgccc ttgaggagcg agccgcggac gacccgcagg
     1321 aagtgggtga cggggatggc ggtgcccagc acctgcgccc agtcgggcat gccgcggaag
     1381 gggaacatga agccggagag caggatcgac ggcaggatgt agaagatcga catttgcatg
     1441 gcctggagct ggcttctcgc cagggtcgag aagaggaagc cgagcgccag gctgccggtg
     1501 atgaagaggg ccgtgccgag cgccagcgct gcccagccac cctcgaaggg cacgccgaag
     1561 aggaggcggg cgaggccgag gatgagcacg gtctggatca cgccgatacc gacatagggg
     1621 gcgagcttgc cgatcatcac ctcgatcggc cggaccggcg tggcgagcag cgattccatc
     1681 gtgccgcgct cgtgctcgcg ggtcacggcg agcgccgtca tcatgacgag cgtcatggag
     1741 aggacgatgc cgagcaggcc cgggacgatg ttgaaggcgg tgatgttctc ggggttgtag
     1801 cggcggtgca cgacgatctc gaaagggcca ccaccgccat cccgtgccag cgggccctgg
     1861 aggtcgtggt cgagggccgc ctcggccacg ccgccgagcg agcccaaggc gctgccgacc
     1921 gcggtcggat cggaggcgtc ggcctcgacc aggatccggg gcgcgtcacc tttcacgacc
     1981 cggcggctga aatcgtgcgg gatgacgacg gcgagctgcg ccgcaccgct ctcgatcgcg
     2041 gcgtcgatag cggcgtgcga ggcagcctcg gcggtgaaat cgaaatagcc cgtgttgccc
     2101 agggccgcga gcaccgagcg ggagagcgtg gtccggtcct cgtcgagcac cacggtcggc
     2161 agatggcggg ggtcggtgtt gatggcgtag ccgaagagca tgagctgcat gaccggcatg
     2221 acgaggatca tcgcgtaggt cagccggtcg cgcgagagct gcaggagctc cttgatcatg
     2281 atggcgagga tgcgggttcc ggagatcaac ggaagttgtc ctcggcaccg cgcatcaggt
     2341 ggataaagac gtcctcgatg gtcgggcgcc cctcggtcca gcgcaggcca tcgccgcgat
     2401 gccgcgagat ggcccgctcg agcaaggcac ggtcggggcc gctgacatgg agcgcggcgc
     2461 cgaagggggc ggccatctcg acgcccggct cgcgcagcag agccggtgcc aggcggtcgg
     2521 cgcccgggcc ttcgcccttg aaggtgacga gggcggagcg gtcgatcagc tcggccaccg
     2581 tgccgcgcgc gatcaaggca ccataggcga tataggcgat gtcgtggcag cgctcggcct
     2641 cgtccatgta gtgggtcgag accatgacgg tcatgccttt cgcggtgagc tcgtggatct
     2701 ggtcccagaa ctcgcggcgc gccttgggat cgacgccggc cgtcggctcg tcgagcagga
     2761 gcaggcgcgg ctcgtgcatc accgcggcgg cgagggcgag acgctgcttc cagccgcccg
     2821 agagggcacc ggccggctgc ttgcggcggg cttcgaggcc gagcccggcg agcgcctcgg
     2881 cgacccgccg acggcggtcg tccatgccgt agacccgggc gacgaagagc agattctcct
     2941 cgatggtcaa atcgtcatag agcgagaacc tctgcgtcat gtagccggtg cggcgcttga
     3001 tctcgccggt ttccttcagg atgtcgaggc cgaggcaggt gccttcgccg ctgtccggcg
     3061 tcaggaggcc gcagagcatg cgcatggtcg tcgtcttgcc ggacccgttg gggccgagaa
     3121 agcccgtgac cctgcccgcc tcgacggtga tcgagacgtc gcggacgaca tgcttgtcgc
     3181 cgaagctctt gttgaggccc ttgacgtcga tggcgaccgg catcggtcag ggcccgagcg
     3241 gcgtcacgtc gacgggttgg ccgggcgcca ggccggcggg gtcgacgggc tcggcctcga
     3301 tcaggaagac gagcttctcg cggctttcct ggctgtagat caccggcggc gtgtactcga
     3361 cgcgcgggct gacgaaggtg atgcgggcgg cgcgtggggc accgcagccg tcgcaggtga
     3421 aggtgacctc gccgcccgtg cggtaggtcg cgaccgccgc ctgggggacg aagaagcgga
     3481 tcttgacccg ctcgtccggc aggagggaga gcacgggctg gctcgccggc acccactcac
     3541 cggcccggaa gaaaacgttc tcgatcagcc ccgcggccgg tgccgcgacc gccagttgcg
     3601 ccaacagcac ttccgcttcg tcgaccgccg cctgcgcccg ctcggcttcc gcctcggcgg
     3661 cggcgaccag gtccgcgcgg gcgccgagct gggcggcgtc gagctggcgc atgacctgcg
     3721 ccagctgcgc atcggcggtg cggtagttgg ccagcgcctc gtcgaggcga gcacgggagg
     3781 tggcaccccg ctcgaccagc ggccggacgc gatcgagctc cagcttcgcc tcctcgagct
     3841 gggccttggc ggcgtcgcgc tcggcctcga tcactgccat ctcctccgcg cgcatgccct
     3901 tgcgcgcatc ctcgacccgg gcctgggcgg cggccgctgc ggccctggca ccttcgagtg
     3961 ttgcctcggc ggcccgctgg tcgatcacga agaggctctg gcctgccgca acccgatcac
     4021 cgcgatcgac gtcgagcctg gcgagctcgg cagccatggg tccggccaga tagagggcct
     4081 cgccctcgac atagccggtc aggagcggtg gggtcgtgac acggttctcg agccaccagt
     4141 ggccgccggc gaccgtgagg gcgatgacga cgaggagcag caggacacgg aggatgcggc
     4201 gtttcatggc gcgccatccg gccgctcggc gagaccgtgg agcagggtgc ggacgtgctg
     4261 ctccgccagc tcgcccagat cgaagggctc ggcaccgccc ggcacgaagg tctcgcgcca
     4321 gagcatgccc atgatgagag ggctcgccac ctgcatggca tagaggcggg gatcgccggg
     4381 gcgcacctcg cctttggcct gggcgcgcgc gatggcggcg gccatgagcg acagcatcgg
     4441 cgctaccacg gcgtcgtgcc agtggcgggc gagctccggg aagttgcggc tttcgctgat
     4501 caccgccttg gccatcgggc cgatgctgcc ggttgccgcg gtccttgcga gcaggcgaac
     4561 gacggtcgac agcagctcgg cgaacgtgcc ctggtgcgct cgggcgaagg cgccgacgcg
     4621 ttccagatcg gcggccacgg cctgcgtcac cactgcccgg aagagctcct ccttggtggc
     4681 gaagtagaga tagagagcac ccttgctcac cccggcgcgc gccgcgatct cgtccagccg
     4741 cgtcgccgcg aagccttttt cgctgaagag ggcgagggcc gcctcgacga tctcgcccgg
     4801 gcgcgcctcc ttgcgccgcc gaaacttcgc cgtggtcacg ccacgtcatc cttaataacc
     4861 gactggtcag ttatataatg gcggtggcct gcactgtcga gccgctcgcc tcgggtgccc
     4921 atggcctgct aggcttcgtc ttgacctcgg gcgggacgga atgatggtga tgaacgacaa
     4981 cgatgccgcg aaggcgatcg cggcagtgaa cggccggatc gtggcagcct tcaaggaccg
     5041 cgacgcggcc gcggcggcgg cctgctacac ggcggacggc aggctgatgg cgcccggcgc
     5101 cgagccgcat gtcgggccgg cggcgatcca ggccagcatc cagtccggtt tcgccgccgg
     5161 tgtcggcggg ctgcggctcg agacggtggc gctcgaggtg ctcggcgaca cggcctggga
     5221 ggaggggatc tacgaggtgt tcgatgcggc cggaagctcc cgcgatctgg gcaagtacat
     5281 cgtcatctgg aaacgggtcg gcggtgcctg gctgatcgcg cgcgacatca tgtcgaccaa
     5341 ccgggcggcc gcctgaacgt cgtcctgccc cttgcgagcc agccggcgct cgctatcgtg
     5401 ccggccaagg aggaaacgac ggcatgacgg ctctgctcga aggacgcaag gcggtgatca
     5461 ccggcgccgg caccggcatc ggtcgggcga ttgccttggc gttagtccgc gagggggcca
     5521 ctgtggcggt gaccgatctc gacgcctgcc aggccgcgcg cgtcgccgcc gaggccgggg
     5581 ctcgggcctt cgccctcgac gtgactgatg ccgccgccac ttcgcgcgtc ctcgacgagg
     5641 cggccgcggc catgggcggc ttcgacctgc tttgcgccaa tgccggcatc tccaccatgc
     5701 gcgccgtggt cgatctctcg gaagacgagt gggacgccaa catggcggtc aacgccaagg
     5761 gcgtgttcct cagcaaccag gctgcctgcc gccgctggct ggccgacggg acgaaagggg
     5821 tgatcgtcaa caccgcctcg ctcgccgcca agcagggcgc accgctgctc gcccactatt
     5881 cggccagcaa gttcgcggtc atgggcttca cccaggcgct cgcccgcgag gtggcgcccc
     5941 agatccgggt aaactgcgtg tgtcccggct tcgtgcgcac cggcatgcag catcgcgagg
     6001 tggaatggga ggcggagctg cggggcatga gcgaggaagc ggtgcgcgac gagtatgtct
     6061 cgctgacccc gctcggccgg ctggaggagc ccgaggacgt ggccgatgcc atcgtcttcc
     6121 tcgcctccga tctcgcccgt ttcattaccg gtgagagcct gaacgtcact ggcggcgtca
     6181 ggaccgactg atcgatgctg gtgcgccggg ccgcagcgac catcctgccg cacgtcgtgc
     6241 tcgtcgtttt ttccgccgcg atcctcctgc cgctgctctg ggtgctcagg gtgagcctca
     6301 cggacaagct caccgcctac cgcattccgc ccgaatggac cgagccccgg ctcgacaact
     6361 acatcgagat cttcacggca tatcgctttg ccgactggtt ctccaacagc ctgatcgtgg
     6421 cgctcgcctc gacactgctg gcgctgccgc tggcggtcgc catggcctat gccttcgccc
     6481 ggcacgacac cggcggagca cccctgcggc tcggcgtcct ggcgagccag atgctgccgc
     6541 cgatcgtgct ggtggtgccg ctcttcgcgc tcttcctggg cgccggcctg ctcaacacct
     6601 atgcggcgct ggtgatcgcg catctgacct tcaacattcc cttcctcgcc tggatgctcg
     6661 tcgccttctt cgagggcgag gtgcggcagc tcgaggaagc ggcacggatc gagggggcca
     6721 ctcgttttca ggccttcatc cgggtctcgg tgccgctcgc cgcacccggc atcatggccg
     6781 cggggctcct gggcttcatc ctctcctgga acgagttcct gctggcgctg atcctttcgg
     6841 gccgcgcgac gcagaccctg cccgtcggcc tctcgtcgct ggagacgcat cgcggcgtcg
     6901 agattgcgct cctcgccgcg gcagcactgc tcgcgatcct gccggtcttc gtcctgctgc
     6961 ctttcctgcg caagtacctg atcaagggcc tctcgctcgg agccctcaag tgatccctcg
     7021 atccaacccg accgcgtctt gcggtccaat caggaggagc cacctcatgc gcaagctgct
     7081 gctcggcacg accattctcg gtctcggctt cggagccgcc gaggcccaga ccctcaacat
     7141 cctcatggag agcgtgcccg acacgcgctt cgtcgaggag ctcctgccgg aattcgaggc
     7201 ggccaccggc atcgacgtcg agctcgaggt cgtcaactac gccgagatgc acaccaagct
     7261 ggtgccgcag ctcgtctcgc cgaccggctc ctacgacgtc atcgtggtcg acttctactg
     7321 ggtcggcgag ttcgccaagg ccggctggct gcagccgctc gacgagcgca tcgccgccga
     7381 cggcttcgac acctcggtct atttcgactc gctgcaggat ctcgtcggcc aggtcgacgg
     7441 cgagcagctc atgctgccct tctacaacta cgccatgggc ctgacctatc gccacgacct
     7501 cctcgaggac ccggccgagc aggcggcgtt caaggagaag tacggcatcg agctgcgcgt
     7561 gccggagacc tgggacgagt atctgcagca ggtggagttc ttcacccgcg ataccgacgg
     7621 cgacggcaag aacgacttct tcggcgtggt caaccagggg ctcaggcccg atccgatcgc
     7681 catggagtgg tcgaactatc tctttgccaa tggcggccgc ttccacgacg agagctggca
     7741 gccgatgctc gacagccccg aggcggtcaa ggcggtcgag gactacaagc ggaacctcga
     7801 ggagttcggg ccgctgggtg ccgcgagctt cggcttcgac gaggcgttca acgttgcggc
     7861 ccagggcaag gcctacagct acctgaccta caacatgttc cgcacggcct atgacgatcc
     7921 ctcgcagtcc gcggtggtcg acaagatgga gatcgtcgcc gtgccgaatg gcgggctcaa
     7981 cggtgcctgg ggctgggcca tccccaactc ctcgcccgat ccggacgccg cctggacctt
     8041 cctcaagtgg gtggagagcc cggagatcgc gaagaagcga gcaatgctcg gcggctcgcc
     8101 cacacgcaag gacgtcttcg ctgacgagga ggtgctggcc aagtatccct actacccggc
     8161 gctcgagaac ctgctcgcca cctcgcacaa cttcccggtc ttcacctaca ccccgcaatt
     8221 cgtcgaggtg ctggggcgcg agctctcgct cgcggtgacc ggcgagaaga ccgccgagga
     8281 ggcgatgcag acggccaatg ccgagctcga ggccctgctg aagcaggacg gcaagctcta
     8341 ggcagcgggc ggggagggcg gcgtggcggc gacgcttgca gcgcgcgacc ggcgcttcgg
     8401 ccagctcatg gcagcgccgg ggctcgccgc cctcctgctc gtcatcgtct ttccgattct
     8461 cttcacgctc tacacgagcg ccttcgactt caccctcatc aacccgcggc acgacacgtt
     8521 cgtcgggttc gcccactatg cccgggcgtt cggcctcgcc gagttcggca acgcgctcct
     8581 ggtcaccttg tggttcgttg tcgcggtcgt cgtgctcgag ttcgccgttg gcttccttgt
     8641 ggcgctcatg ctcaacgccg tcgagcgcgg caaggccgtc tactacgcca tcctgctctg
     8701 tccgctcctc atcaacccgg tcgtggtcgg actgatctgg cggatgttcc tccatccgga
     8761 gctcggcatc gccaactacg tgctcggcct cgtcggcctc gggccggtca actggctggg
     8821 cgatccgcgg aacgccttct ggaccctcgt ggtggtcgac atctggcacc aggtctcgtt
     8881 catgatcgtg ctgctgctcg ccgggctcgc ctccttgcca aaggagccct acgaggcggc
     8941 ccggatggag ggggccggca cgctcgcctg cttctggcac gtcaccctgc cgctcatgcg
     9001 cccggtgatc ctggtgacgc tgctgatccg cctgatcttc gccgttcgca cctatgatct
     9061 cgtctacatc atgacccgcg gcgggcccgg ccaggcaacc gatctggtca gctacttcat
     9121 ctaccggcag gccttcgtcg gcctcaatat cgggcaggcc tcggcgctcg ccgtgctgct
     9181 gctgctcatc gtcctcgttc tcacagcctg gctctaccgc cacatgcgat cgctcgtctg
     9241 atgcccgata tccgcctgag caacgtcacg aaggtctatg gcggcaatgt cgtcgccgtg
     9301 gacggcatcg acctcgagat cgaggatggc gagtttttga tcctgctcgg cccctccggc
     9361 tgcggcaagt cgacgacgct gcggatgatc gccgggctcg agaccatcac ctccggcgac
     9421 ctttttgttg gcgagcggcg ggtcaacgag gtcgacccca aggaccgcga cctcgccttc
     9481 gtcttccaga actacgcgct cttcccgcac atgacggtgg cgggcaatct cgccttcggc
     9541 atgcgtctgc gcggcgtgcc gaaagccacc atccagagcc ggatcgccga agcgacggcg
     9601 ctcctcggcc tcgacggcct gctccagagg aagccgaagg agctctctgg cgggcagcgc
     9661 cagcgcgtgg cgctcggccg ggcgctcttg cgcgaccctg tggccttcct cctcgacgag
     9721 cccctctcca atctcgacgc caagctgcgc gcccagatgc gcaccgagct ggtcaagctg
     9781 catcgccgcg tcggccgcac catcatccac gtcacccacg accaggtcga agcgatgacc
     9841 atgggcgacc ggctctgcat catgaacggg gggcgcatcg ttcaggtcgg gcggcccatg
     9901 gacgtctacc gcaaccctgc cgacacgttc gtcgccggct tcctcgccag cccgcccatg
     9961 aacctcatcg agggccgaat cgagaacgag cggatcgtcg tccagggcct cgaattgccg
    10021 ctcgccggct tcgcgccgaa aagcgccgac atcgtcctcg gcatccgccc cgaggatctg
    10081 gcggcggtcg ccatgggccc cactgccgtg ccggtcgatc tcgacgtcgt ggcgatcgag
    10141 gcgctcggac ccgagctggt cgtgatcggc gggctcggtg ctggtggcgc ggagatcgcg
    10201 gcccgactgt cgcccgattt cgtggcggcg atcgatgctc cctgccggct ctggctggat
    10261 ccggcgcgga tagcactctt cgacgccgcg acgacgcgcg ccgtggcgcg gagctgaagg
    10321 tgtcggccca cggcgctcga cagcggccgg ggaggtggta cggttcggat gtgccgcgat
    10381 gagcggcgcc aactcagcgg ggggaacagg aaggatgtcc aggaaccgac ttttggcctc
    10441 ggccagcgtg gtggcgctag gccttgttgc ggcgggcggt gctgtcgccc aggatctgaa
    10501 gccgaccagt ggcgagaccg tcttcttccg tggctggcag tacaagaccg acatcgtcca
    10561 gagcaatgtc gaccgctaca acagcgagct cgacggcaag gtcgactatg ccacggtgac
    10621 cggcgactac cccgcgatca tggagaccaa cctgatcgcc ggcgccgagc tcgacatcct
    10681 gtacgccaac ccgtcgcagg ccgcgcgcta ctatgacggc ggctgggtgc tgccggccga
    10741 gagcctgccg aacatcgacg agatcaaggc ggacatgtac cccaacatcc tcgacgcctg
    10801 gacctacaag ggcgagctga ccggcctctc ctatttcgtc agcacgcgcg gcgtgatgca
    10861 cgtcaacctg aagcagtatg gcgagctcgg ctacaccgat gccgacttcc ccgccacctg
    10921 ggacgagctc tacgaccagc tctacgagat ccgcgacaag ggcgtggagc agccctattt
    10981 gccgcactgg ctctcggagt ggtacggcat ctcgtggggc ttcgtcttcg aggtcatgaa
    11041 ccgcggtggg gatttcgccg accccgagac gcaccagccg acgctcgaca cctccgaggg
    11101 cggcccggcc tgggcgacgc tcgaggactg gaagcgcatc ttcaacgacg ggctcgtgcc
    11161 cgaggaggtg ctgacctaca acgagagcgc ctatatcgac gcctatgctt ccggccgtta
    11221 cgtcttcagc ccgcagcagc tctacgatct cgagacgttc aaccggcccg atcgctcgca
    11281 gatcgcgggc gaggcgagcc tcctgccgta tcagggccag agctggggcc tgctcgacag
    11341 cgccgtctac ctgatgacgt cgcgcgagcg tgacgaagcg cataccgacg acgtcaagaa
    11401 gttcgcctcc tggtacggct tcaaggatca gaacggcgag attttcgttg gccagcgctg
    11461 gatgcaggaa tcgatgctct tctccggcta caagtcggtg atggagagcg aggccacggc
    11521 cgagcgcatg aagggtgccc tggcccggcc cggcgatgtc gaggcggtgc tcggggtcta
    11581 cgccgagacg ccctacccaa agggcatctg gaatgtcgtc tggtcggagg agttcaactc
    11641 ctggctgcgc gaggagctgc aggcctttat cctcgacgat ggcgacatcg gcgacatgct
    11701 cgaatcgatc aacgagaaga tcaccgagct gaacgccaaa tacgggatct gatcctcaag
    11761 gcctgatctg ggggcggcgg ctaccagctc gtgtagccgc cgtcgaccag gaggatgctg
    11821 ccggtggtgt agctcgaggc tggagaggcg agaaagagca cggcgctcgc gatttcctcc
    11881 ggctcggcga accggcccat cggcgtcatc tcggcccaga tcctggccca ttcgctattg
    11941 gcgagcccga gcttcgtcat ctcggtgagc gtgtagctcg gggccaccgc gttgacccgt
    12001 acccccttgc tcgccagctc caccgccagt gacttggtca gcatgtgcac cgcacccttg
    12061 gacgcgttgt agtgcgcctg gggttgcggc ttgtcggcga tcacgccgga gttcgagccg
    12121 atattgacga tgctgccgcc gccggcctcg gccatccgtc taccgaaggc gcggcaggac
    12181 cagaacatcg cgttgagatt gacgtcgatc acctcgcgcc actcgtcgtc ggggatctcc
    12241 gtcacgggcg tgttgcggca gatgccgacg cagttgacga ggacgtcgat gccgccggcc
    12301 tcggcccaga ccgcttcggc catggcctcg acggcggcgg cgtcgcgcag gtcgagggcc
    12361 ctgtgtcgag catcgagccg gccggcagct gccgccacga gatcggcctc acggtccacc
    12421 agccagagcg ccgcaccggc atcgcggagc gcctcggccg aggcgaagcc gatgccgcgc
    12481 gccgcaccgg tcaccaccgc ccgccggcca tccagcctga accgctcgag ataacccata
    12541 atccattccc tttcgccgca ccgtgtccgg cacgtgcttg acgccggcca accccgcccc
    12601 gaacatggcg ccaatcgagg ggttgcggca accttgcccg aacgtccccg aggaacaggc
    12661 cggcaaaccg gctcgatgag ggggaacatg tggcaggtca ggtgaccagc accggccgga
    12721 cgagcgagct atcgaacctg cagttcgcgc tcttgatggc gctgccggtg ctgctcttcc
    12781 tcgtcctcgt ggtggcctat ccgctcggct acgcgctctg gatgagcttc cagcagatcg
    12841 tcttcttcgg cggctaccgg acgaatttcg tcgggctcgc caactacgcg acgatgctcg
    12901 gcgacgacaa gttctggtgg tcgatctggg tcaccgtccg cttcaccgtc gagtccgtcc
    12961 tgctcgccat ggcgatcggg ctcgctctgg cgctcctttt gaaccggccg atgcgtcggc
    13021 cgggcctgat ccgcacgatc atcatgctgc cctggtgcgt ctcgctctac ggcaccggca
    13081 tcatgttctc ctacctcgcc accgggcaga ccggcttcgc caccgccatc gccttcttct
    13141 tcggcgccga ccggccggtc agcttcgtca accggcagtg ggtgatcgag gtgctggcca
    13201 tcggcaatgc ctggaacatg gcgccgctcg tcgcgttctt cctgctcgcc aacctgaaga
    13261 cgatcccgac ccggctctac gacctggccg ccatcgaccg gatgtcgccg ctcgagacgt
    13321 tctggaacgt caccctgccg ccgctccgct tcacgctctt cgtcttcacc tgcatcacca
    13381 cggtgctgtc gatgaagctc ttcgacttca tcttcgtgct ctccggcggt ggccccggga
    13441 cggcctcggc ggtgatgacc taccagatct acaagatcag ctttcgcgat ctcaatctcg
    13501 gctacggcgc cgcacagtcc ttcgtcctgc tcttcctcat cgtcggctcc accatgacgc
    13561 tctatgcgct ctggggaagg cgcgaggcgg cacgctgatg cagcggcgca tgacgctctg
    13621 ggcgtggctc ggcatcgcgg tcgccgtggt ctggacgctc ttgccgatct actggttctt
    13681 gaagatgtcg ctgctcacgc ccgacgagat cgcgcggttc ccgccgccgc tcgtcccgct
    13741 cgatccgacg ccggcggcct atttcaacat cttcggcttc gactaccagg tgacgcccga
    13801 gctcgtgcgc cgcgcctcgg gtcaggcggg gcagatcatc aacggcctga tcaacagcct
    13861 catcgtctcg atcgtcgtga ccgccatcac catgctcgtc gtggtgccgc tcgcctacgt
    13921 cttcgcccgg ctcgacttcc gcttcaagca ggcgctcctg atcaccatcc tgctctcggt
    13981 ggcgctgccg ccggtctcga cgctcattcc cttctatgcc ctctacgtgc agctcggcct
    14041 cgccggcacg ctcttcgggc tgatcgtcgt cacgctgacg atcaccattc ccttcgtcac
    14101 ctggatgctc atcggctatc tgcgcaacct gccgccggtc gagcggctgg cccgggtcga
    14161 cggctacacg cggctcgaga cgctggtctt catcgtcatc ccgctggcca ggagcggcat
    14221 cgccgtcgcc gccgtcatcg ccttcctctt cgcctggaac gagtacacct acgcgctcgt
    14281 gctggtgaac ggcacgccgg ccaacaccct ggcgacggcg ctctcgggct tcctcttcca
    14341 gcaccccgag ccctcgcatc tggccgcggc cctcgtggtc tccatcctgc cgccggtgct
    14401 cgtcgcctat ttcctgcagc gccacatcgc cgagatgaac ctcgtggatc cgatccgttg
    14461 acggccgatc cgctgaagcc accagggacc tgacatggcc ggcatccgcc tcgaccacct
    14521 gaagaagagc ttcggcgcct tcaccgccgt ccaggatctc gatctcgacg tcaccgacgg
    14581 cgagttcctg gtgctgctcg gcccttcggg ctgcggcaag acgaccacgc tcaactgcat
    14641 cgccgggctc gagacgccat ccgcgggccg catcctcttc gacggccgcg acgtcaccag
    14701 cgacccgccg cacagccgca acatcgccat ggtcttccag tcggcgctgc tctacccgca
    14761 catgacagcg gcggccaata tcgaggccag cctgcgccgc tccgacctcg cccccgaaga
    14821 gcgccgccgg cggatcgacg acgccgtttc gatgctcgag atcggcgacc tgctcgacaa
    14881 gctgccgagc gagatgtccg gcggccagcg ccagcgcgtc gccaccgcca aggcaatcgt
    14941 ccgccagccc acggccttcc tgctcgacga gccgctctcg gccctcgacg cggcgctgcg
    15001 catgacgctg cgcgccgaga tcgtgaacct gcagaagcgg ctcggctcga ccacggtgtt
    15061 cgtcacccac gaccaggtcg aggcgatgac catgggcgac cggatcgccg tcatgaatgg
    15121 cggccggctg gagcagatcg gcacgccgag cgagatctac gagcggccac ggagcctctt
    15181 cgtcgccggc ttcgtcggct cgccgccgat gaacctgatc cggggcgagg tggccgacgg
    15241 cgtgttcgcg gcgggcgagc ttcgcgtgcc gctcgcggcg gcaccaggcc ctgtcaccct
    15301 cggtgtgcgg ccgcagcacc tccacgtccg cgccgagggc ggcctgccct gcgccatctt
    15361 cgcgctcgag cgcctcggcc gggagaccgt gctcatcgtc gaggacaggg cgcgcaaccg
    15421 tctacggctg ctggtcgagg cggagttcga aggccgggtc ggcgatccgt tggacgtcgc
    15481 ccccgatccc gcccgctgcc ttctcttcga cgacgcgagc ggcctgatcc acgcaccagc
    15541 caactgaagg aacggacatg gcaaagctgc gcatgggtgt cgtcggcctc ggcttcatgg
    15601 gctcgctgca cgcgcgcctc tggcacgagc tgccgaacac cgagctcgtc gccgtggcgg
    15661 acctcgaccc ggccacgggc gagccgctcg ccaggaagta tggctgtgcg ttccatgccg
    15721 acccggccga cctttttgcc cgcgccgaca tcgacgccgt cagcatcgtc acgcccgacc
    15781 gccaccatgt ggcacccgcc actgccgccg cggccgccgg caagcacatc ctgctcgaga
    15841 agccgatggc ccacaccggt gccgcggcgg ccgagatcgc ggccgcggtc gagcagcatg
    15901 gcgtgcggct gatggtggcc cacgtgctcc gcttcgatcc gcgctatgtt cagctccggg
    15961 ccgccgccga tccggagacc ttcggcgagg ccatccatct gcgcgccaag cgcaacagcg
    16021 tgcgcggcct cgcgcaaagg ctcggtgcca attcctctgt cctctattat ctcggcgtcc
    16081 acgacgtcga catgctgcaa tgggtggccc gctcgccgat cacgcgggtc tatgcccaga
    16141 aggtgcagaa gctctcgaac ggcaacgagg acgcgctcta cgccgtgctc aacttcgcca
    16201 gtggcgccat tggcacgctg gactacagct gggcctggcc cgacggcctg cccaacggct
    16261 actgggtgca tctcgaggcc gtcggcacga agagcgccgc cttcctcgag gtccgcgacc
    16321 agggcctgca cctcgtcggc gagaccggca cggtgggcgc cgacacccat ctctggccgg
    16381 agatcaacgg caagatcatc ggcgacctgc gcgacgagct gctgcacttc gccgaggcgg
    16441 tgcagagcgg cgagcccttc gtccaggact ggcgggacgc ccaggacgcg atccgcgtgc
    16501 tcgatgcgct cttcgcgtcg gtcgaccagg gtgctcccgt cgacgttcga cgctagccct
    16561 tgacggcacc cgccagcagc ccctcgacga cataacgcca gaccagcacg accaggagca
    16621 gggtcggcag cacggcgatg aagatggcgc tgttgagcag gctccaggag acgtcggtgt
    16681 tgcgtgccag ggccgccatg acgaccgaga gcggccggga gcgggcgtcg cccgagagca
    16741 ccatcgagaa caggaactcc gtgtaggaga gcatgaaggc gaagagcccg gtggcgcaca
    16801 ccgccggcaa agcgaggggc agggccaccc ggaggaagct ctgcaggcgg ctgcagccgt
    16861 cgattaccgc cgcctcgaac agctcgtccg gcagcttccg gaagaagacc gagaggatga
    16921 ggatggtgaa cggcagcgag cgggcggtat gcaccagcac cacagcgagc ttggtgccga
    16981 gcaggtcgag gccgtccacc agcaggtaga tcggcgtgac gagggccacg gccggcacca
    17041 gcgggctgag gatcaaaaag aggaaggccg gcttcttgcg cgggaaggcg aagcgggcga
    17101 aggcgaaggc ggccggtgcg ccgagcagca ggttgagggc gaggacgctg gtcgagatct
    17161 gcgccgagtt ccacaggctc cgcggcacct gtcgggccgc gtcggagatc atggcccggt
    17221 tcgcgccctc ggccatgtag gcggctggca gccggcctgt gaagatgtag cggtagttgt
    17281 cgagcgtcat gccgccttcc aggaccgagc ggtcgcgatc gaagaggatc cggtcgggca
    17341 cgacgctgcc gaaggcggcg agcaggatgg ggccggcggc gaaggcgagg acgagcaatg
    17401 ctgccagatt cacgatcagc cggtcggcca gccggccggt cacggcgcct cgtcctcggt
    17461 cgcggcgaag agatcggagg gcagggcacg ggtgatggcg acgatcatca ggccggccat
    17521 ggcgacgacg agaaaggcga ccgccgagcc ggcgccgaaa tcgtacatcg agaagctctc
    17581 ccgccagatc tggaaggaga ggagggaggt ggcggtgccg ggaccgccgc cggtcatggc
    17641 gtagaccacg tcataggtcg tgaaggcggt gagcgcgttg tagacgagga ggaccacgac
    17701 catcgccttg atcgccggcc agacgacgaa gcgaaagcgt tgccaggcat tggccccgtc
    17761 gatcctggcg gcctcgaaga gggcgggatc gatggtcgag agggctgcca tgatcagcac
    17821 gaccgagaat ggcacctgcg accagatatg ggccacagcg accgaggcga aggcgaggct
    17881 cgggtcgctt agccagggga tcgagctggt gatcaggccg agatcccgca gccaggtgtt
    17941 gagcatgccc cagctcgggt tgaagaccca gatccagagc acggcggtga tggcaccggg
    18001 caggacccat ggcagcagca cgaccgagcg gagcacggcc cggccagtga atcgccgctg
    18061 cagcaggagt gccgtcgcaa tgccgaggcc gaccacgacg ggggcggtga ccgcagtgaa
    18121 caggagcgag ttcttcagcg tcgtcagaaa gaaagggccg gcgatcacct tgcggtaatt
    18181 gtcgagcccg gaccagctca gctcgtccgg aaagaggaaa tgcgccgagt gcaggctggt
    18241 ccagagcgtg tggccgatgg gcacgacggt gaagagcgtg acgaggagca acggcgcgcc
    18301 cacccagagg acggggaagc gcgccgatcg ccacatgcct cgctggtcgc cactcaccgg
    18361 caggcggccg aaacccgacc ggtcagcgcg ccatttgtgc gcgaagcgcg tcccagcgct
    18421 cggccccctg gctcatgacc tcctcgaccg tggcctcgcc gatcatcgcc tgcgccatca
    18481 ggggcctgaa gctcgcggcc cagacggcgg tccattcctt ccaggtgccg ccgcgcgcgg
    18541 tctcgatctg cgcctgcagg gtcggcatgt cgacccagcc gctccacgcc tcgatcacct
    18601 ccggatcctc gagcaatggc aattggccga agcccaggcc cttctcgatc gcccagcgct
    18661 tggccaccgc gtaaggcggc ccggccatga aatcgacaaa cttccaggct gcttcgcgcc
    18721 gctcccggtt ctccgctgct gacgacgtga tcgcgtagaa cttggtgaag ccgagcgtcg
    18781 actgcgcctc gcccggcatc cgcgccaacg cgaactcgcc ggcgagcggc tggtccgcct
    18841 tgttgttcat ggccgccagc acgtagttgt agacgatggt gaaggcgtgc ttgccggagt
    18901 tcatcgccgg gatgatggtc gattcgtggt tctcgaaggc gaccaggccc tcggcaatga
    18961 catcgcgcag ccagctcagc tgctggaagg cggcattgtc tggccggttg aagatggcct
    19021 cgccttcctc gtcgaaaagg tcgccgccgc ggccgtaggc ctgcgcgacg aaggcatcgt
    19081 agaagttcgg caactcttga ttgaactcgt agacgatcgg ccgctccatg ccgttggcct
    19141 tgagcttgcg agcggcgtcc gccacttcct cccaggaggt cggcacctcg atggcggcat
    19201 ccttcaggat tcgctggttg tagatgaacg agatggtgtc ggcgtagtag ggcaggccgt
    19261 agaggtggcc gtcataggtc atgtcgttga tggtgaaccc ggcgaggtcc ttcttgtagg
    19321 actcgatcgc gtcggcagcg atctcgtcga gcgggtagag atagccggcg gccgcccagg
    19381 ccggcagcca gtcctggccg ccatagatca cgtcggtggt ggtg
//
LOCUS       DAOTFI010000075        19391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3384_length_19391_cov_6.870087, whole genome shotgun sequence.
ACCESSION   DAOTFI010000075 DAOTFI010000000
VERSION     DAOTFI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19391
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3384_length_19391_cov_6.870087"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /gene="treZ"
                     /locus_tag="P5558_09540"
     CDS             <1..1654
                     /gene="treZ"
                     /locus_tag="P5558_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HRY24593.1"
                     /translation="MRLVGDGWFTAETPLQPGDAYAFRLSEGSVVPDPASRAQQGGVH
                     GPSLVPGPIATDPAWRGRPWHEAVLYELHLGTFTPEGTLAAAAAKLPYLADLGITAVE
                     LMPLAAFAGSRGWGYDGVLPYAVHESYGTPEDLAAFVARAHAHGLMVFLDVVYNHFGP
                     DGNYLGAYASRFFRDDVSTPWGAAIDFREPAVRAFFIENALFWLETYGIDGLRLDAVH
                     AIVDEGEAHLLAELARAVRQRFGGERQVHLVLENEDNIAGWFDGRYDAQWNDDWHHVA
                     HVIATGETDGYYRDYAEDPVGLLLRSLAGGFVYQGEPSPHRDGAPRGEPSAHLQPTAF
                     VDFLQNHDQIGNRAFGERLTRLAPVEAVAALQAALLLSPHVPLLFMGEEWGATTPFLF
                     FCDFEGPLADAVREGRRSEFGKFAAFADPAVRATIPDPLDLATFESSRLDWSEGEQAD
                     HAERRRLVRDLLAIRREHIVPGLAGMAPGATIVRHGTRGFTLAWQLTNGSVLSLQANL
                     GGEPLSGFPGLPGRCLHATPGTDPSADRRPAWSVAASIDQRP"
     gene            1651..3447
                     /gene="malQ"
                     /locus_tag="P5558_09545"
     CDS             1651..3447
                     /gene="malQ"
                     /locus_tag="P5558_09545"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRY24594.1"
                     /translation="MSGDLDVRARAAGIEPGYYDLEGNWHVASPATKAALLEAMGPLD
                     GAAEPPQGAFTPAVTPAACGLGRIWGVACQSYGLRSPRNAGIGDFADLERLGTGLATR
                     GADLLGLSPLHARFRDQPARACPYAPSSRLWLDPLAIALDAAAADLGLELPALPAPPR
                     GELVDYPAVAALKEQAFAALHERFAKGHPALADFREWRIAKGAPLESFARFEAIALAL
                     RARTGRPVAWPDWPVELRRVDAPGVAAFAIEHANEVERSAFLQWLAERQLTRTQQALM
                     DAGMRIGLYADIAVGVVPDGADVWADPEAVVAGATIGAPPDAFSPKGQNWNVAPLSPV
                     ALRHRDMAPLRAILTAAMQHAGAVRIDHAMGLMRLFWIPAGGTPADGAYVRYPLQHML
                     ATVAEVSRAHACIVIGEDLGTVAPGFREAMLEAGLLSYKVVWFERWESGLFRSSNLYP
                     VASLATVSTHDLATVRGYLQGRDIDWRETTRQLDAAAAAAARDERRADCQKLRDALRV
                     EGLLDEDQPAADEPEAIVLALHRFLARTPSAIAMACLEDLDGAIEQPNLPATIDEHPN
                     WRRRTIHDIAGLLGSAPAQRLIQALAEERPRD"
     gene            3440..5908
                     /gene="treY"
                     /locus_tag="P5558_09550"
     CDS             3440..5908
                     /gene="treY"
                     /locus_tag="P5558_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HRY24595.1"
                     /translation="MIETVRATYRIQLRGGVDFATIASAVPYIARLGISHLYLSPIWQ
                     ARAGSTHGYDVVDPNVIEPALGGEAGFRDLVAEAKSAGLGIILDHVPNHMGVGSDNAW
                     WWDVLLNGRASRFAGHFDIDWAATAGATPGRLLLPVLGASYGEVLASGDLRVEAAAGG
                     FELRYHDQRFPLRADSVPNGQAELHALLERQAYRLAYWRLGGEALNYRRFFDIDGLVG
                     VRVEDETVFADAHRLLLELVAEGAVQGVRLDHIDGLRDPTGYLHRLDTALREATGGAP
                     PPVWIEKILEGGETLRPDWPVAGTTGYEAADRLARLFVAGDGMGTLDALWRQVAEGEA
                     DFAAMLRDCKALVLGASFAGELEWLVASALHLAAGDARFRDTGRASLTRALTALILAF
                     PVYRTYLDGASPDAADQQRLAAVLSEARQRAGLADDLPFALVEHILDETTAKAAALDW
                     AARLQQLTGPIMAKAKEDTAFYRHIRLVALNEVGGEPDHPVLAADDFHAFAAAMPPLC
                     QLAGSTHDSKRGEDLRARLIGLSHHAEAWQEAVAGWLDTKPDSIAASDACLVWQTIAG
                     AWPDDLEPGDAPGRKAFADRLDAYLVKALREAKARSSWTTPDEAYESAVQAFAASCLE
                     GATGRALHDFRGRLLPEGIARSLAQTALRLAMPGIPDIYQGAELWNLGLVDPDNRHPA
                     DFTHLAAQLAEPSPDFGHDWRRGRVKQFLIQRLLADRAARPALYAEGAYVPLEVGGSA
                     VAFARLAADDALLVAVPIRADPALPPGEAPRFGPQFAGTRVALPSALADRKWRCLLTG
                     RETDGPLDLGELLVDWPVVVLASR"
     gene            complement(5905..6831)
                     /locus_tag="P5558_09555"
     CDS             complement(5905..6831)
                     /locus_tag="P5558_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRY24596.1"
                     /translation="MRGAAFLGTFLVAAAAVAQTPPRVVATILPVHGIAAAVMEGVGE
                     PALLLEPGASPHSYSLRPSQAAMLEEADLVLWVGPGFEAFLTGPLETLGAGGHRLALA
                     EVAGVERLGFREGAMFEDHAAAGHGHEEAHEHAHGGFDSHVWLSPANARVMAQAMAAE
                     LGQIDPANAERYAANAAAFGTALDTTEAEVEALVVPVRGRPFVVFHDAYHYFEHAFDI
                     EAAGAIQVNPELQPGAARIAEIQERLGELEVACVFTEPQFEPRLVATLIEGTKARTGV
                     LDPLGVALEPGPRAYHELLLGLARNLAECLAD"
     gene            6950..7402
                     /locus_tag="P5558_09560"
     CDS             6950..7402
                     /locus_tag="P5558_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRY24597.1"
                     /translation="MAAFPADRVEQAAWICNARGQTLTPLRRRVFEIVLAADRPIGAY
                     DVLDMLAAERGRVAPPTVYRSLDFLVGQGLVHKVLSVNGFVACSAAARPHDAQLFICG
                     SCGSSVEVARQLRAGLIEEEAEALDFAIESVVLEVHGRCHACRLATAP"
     gene            7399..8157
                     /locus_tag="P5558_09565"
     CDS             7399..8157
                     /locus_tag="P5558_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRY24598.1"
                     /translation="MSRLLVEVVGASVRLGDHQALENVDLALHGGEIVSLIGPNGAGK
                     STLVKLVLGIIEADSGRVTRAPGLRFAYMPQRLAIDPTLPIDVDRFLDLPHRHPAAER
                     QAALARVGIAGLGRRPLQALSGGELQRVLLARALLRRPDLLVLDEPAAGIDYQGQAAF
                     FGLIDSVRREQGCGILVVSHDLHLVMAATDRVVCLNRHVCCSGTPEAVGDDPGYLALF
                     GPRTAAALARYHHHHDHEHALSGEVAHPTDCGRR"
     gene            8157..8963
                     /locus_tag="P5558_09570"
     CDS             8157..8963
                     /locus_tag="P5558_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRY24599.1"
                     /translation="MLPEFLLRGWLAGLALAAVAGPLGCFVVWRRMAYFGDTLAHGAL
                     LGVTLGLVTGIDLMPAVAVTSALIALLVVLMQRQRTLATDTLLGILAHSGIAIGLVLL
                     GLVGAVRVDLLGYLFGDVLAVRWQDVAVAVIGGAAVLAVLRHYWPQLLFATLHDEMAR
                     AEGTPTTRLRLVLMLLVALVIAAAMKIVGILLITSILIIPPAAARQLSATPEAMAVIA
                     AGLGGLAVTLGLALSWQADTPAGASIVVMALAIFVATQAIGPLVARRLGC"
     gene            8999..9793
                     /locus_tag="P5558_09575"
     CDS             8999..9793
                     /locus_tag="P5558_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24600.1"
                     /translation="MASAEQRIAGIKPGLRVCVTAGAGGIGRAIADGFIAHGARVVVS
                     DVAEGALADFSRAHPDHHAVAADAGSEAATRALFGTVEERLGGLDLLVNNAGIAGPTA
                     PVEEIATEAWQATVDINLNGHFYALRAAVPLLKQTENGSIIMISSVAGRLGYAYRLPY
                     AATKWAIVGMAKSLSLELGSFGIRVNAILPGIVEGPRMDGVIRDRAATTGVSFEEMRE
                     TYLSKTALHRMVTARDIADMALFLASPMGRNVSGQALSVCGDVVSI"
     gene            9805..10749
                     /locus_tag="P5558_09580"
     CDS             9805..10749
                     /locus_tag="P5558_09580"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRY24601.1"
                     /translation="MSETVAIVGTGLVGRAWAIAFARAGWQCRLHDTAPGAAAAARER
                     IGPLVDDLVEAGLLDDAAPVLARITVADDLASALADVAWVQESVPEDVAIKKALWAEL
                     DRLAPADALLVSSTSAIVPSRFTENLAGRHRCLVAHPLNPPYVIPAAELVPAPWTDDA
                     VMQRARAVMESIGMAPIVMKKELDGFVMNRLQGALLEEAFRLVDEGYCSAEDVDRGLK
                     DGLALRWSFMGPFETIDLNAPGGVADYCRRYEQIYQRLQPTMLHRADWNGPVLERIVA
                     ERRAKVPQTALADRQRWRDRRLMALTAHKRNAARDIGD"
     gene            10751..11683
                     /locus_tag="P5558_09585"
     CDS             10751..11683
                     /locus_tag="P5558_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRY24602.1"
                     /translation="MAGPEKVIITCAVTGAIHTPSMSPHLPVTATEIADAAIGAAEAG
                     AAIVHLHARDPETGRPDQTPEAFSPFLQVIKQRSDCVINITTGGGPAMTIEERVKPAA
                     TFAPEVASLNMGSMNFGLFPMLNRFKTFKHDWERQQLESSRSYVFKNTFADIEYILTT
                     CAENNTRFEFECYDISHLYNLAHFLDRGIVKTPLFVQSVFGILGGIGPHPEDVLHMKR
                     TADRLFGTSYRWSVLGAGRNQLPIAAMSAAMGANVRVGLEDSLWLGKGRLAESNAAQV
                     KAARQIIEGLGREVATPDEAREILQLKGGDQVNF"
     gene            11683..12564
                     /locus_tag="P5558_09590"
     CDS             11683..12564
                     /locus_tag="P5558_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY24603.1"
                     /translation="MAVAGPAQARPGVGIFWMCLSAFGSSSMNGIVREATTEMHAFEV
                     AFFRNVFGLVALAPMMLKAGLRATLRTKRLHLHAARGLLNAVAMLSFFYAVTVTPLAT
                     IAALAFTAPLFAALLAIPFLGERPGWRRWAGLIVGFAGALLIIRPGFTDVGIGVLLIL
                     LSSLVWSGALIIIKILARTETSLTITTYAGLFLTPITGAAAWAFWIWPDATGWLMMIA
                     IGILGSITQWSVAQAFHEADATVVLPFDFTKLIWASVIGYVFFGERPDPMTLVGGFVI
                     LASITYVAYREREIRSS"
     gene            complement(12568..14376)
                     /locus_tag="P5558_09595"
     CDS             complement(12568..14376)
                     /locus_tag="P5558_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRY24604.1"
                     /translation="MRKAPKILGEASVGFVRYGRSLLLYHLFFTILTVVALVPTTSLV
                     LTALLTTSGEPAMTNAEILDFLLSPWGLLWALAAATVTVTLITLDEAGMIVIAAAAPR
                     GRYRAATAALWHVVRLAPRLLGLTFVRVAIQAAVTLPFVAGIAWLFVWRLGDYDIYFL
                     WTVRPPVIWPFLAMAAPLALGLVLANAFLYLRWSLAPPAVLLENLGALSALRRSAALT
                     RGRLARTILVVLPVTVAALLLPLLSAFLVDRTAALLLGNLPHRMALVVPATLFVIALA
                     IFGAIAATYLGIAANALVLQALHRRLSGTGRPALAAMPQGGGRLALWAVELALLGFVF
                     VQAAIVLAGFDLTDEVGIAAHRGSSMAAPENTLPAIRRAVDDGADYVEVDVQETVDGV
                     PVLLHDRDLRRVASIDRRIWDLSAAELSAIDVGGWFDASFAGVPAPTLDAAVLAVGGE
                     AKLYIEIKTAPQMPGLVGNVVARLQALDAIEGTIVASFDRAVLDEVGVLEPRLRRALL
                     VYSTIGEPDRRDYDILSIRASALTPEQVVRAHRLGYEVHAWTVNDRREMSRLIDMGVD
                     VIITDLPGELRSLLAERAALRPDERLVVKLRSWLRF"
     gene            complement(14373..15248)
                     /locus_tag="P5558_09600"
     CDS             complement(14373..15248)
                     /locus_tag="P5558_09600"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRY24605.1"
                     /translation="MTISGKARLAGVLGWPVGHSKSPLLHNFWFERHGLDGVYVPLPV
                     APEDLAPVMRALPRMGFLGCNVTLPHKAAMLDLVDAVDPLAAAIGAVNTVLVGDAGSL
                     TGYNTDGLGFLAHLDGCAPDWRAATRRVVLLGAGGAARALAFTFLAAGIHELALVNRT
                     PERAAALVARLGDQNDAAVLALPWAERHRALEGADLLVNTTSLGMVGQPALDLDLAAL
                     PATAIVADIVYTPLSTPLLSEARRRGHRTVDGLGMLLHQAVPGFTHWGGTRPVVDEAT
                     RRLMLAPPVAEPKAG"
     gene            15269..15751
                     /locus_tag="P5558_09605"
     CDS             15269..15751
                     /locus_tag="P5558_09605"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY24606.1"
                     /translation="MSFGGRLADAFWRNAYRHGYPCARLVWRLTNPSTRGVSVAIRHG
                     DRLLCIRESYRPGLSLPGGGIDRGETPLEAAQREAREEVGLDLAAEAFVELGRLDFNA
                     DDRTHLVTFFALDLPSMPAPTVDRREIVWAGWLTRQELADAERQHTIDLYVRHFAGDL
                     "
     gene            complement(15752..16228)
                     /locus_tag="P5558_09610"
     CDS             complement(15752..16228)
                     /locus_tag="P5558_09610"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HRY24607.1"
                     /translation="MKLALQAFDPATFAAFGSVVEPPAPKGNADLLAHLGEGGPGTAI
                     CVKLDTHPAGVLPMQARRMESHPLTEQLFLPIGPARYAIGAALPGPDGAPDLATLKGF
                     LVSGGTGICYRRGVWHLPITILDHASTFLMMMAATGDASVDTAWAELATPLDVGPA"
     gene            complement(16225..17172)
                     /locus_tag="P5558_09615"
     CDS             complement(16225..17172)
                     /locus_tag="P5558_09615"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY24608.1"
                     /translation="MPRLDASARGVFIIAATPFTEDGRLDLAATDRLIEFYLEQGVDG
                     MTILGMMGEAPKLTEEEARTFALHVLGRVDARVPVVVGVSAPGFASMRALGSVVMDAG
                     AAGLMVQPPAVSARGDDAVLGYCRMVAETLGEATPWVLQDFPLVASVPMSALLIQRIV
                     AACPNAVMLKHEDWPGLDKLSDLRGAEAEGARRVSILTGNGGIFLPFELARGADGAMT
                     GFAYPEMLVEVCRLFARGESEAMLDLFDRYLPLVRYEQQPGLGLAVRKYVLWRRGALA
                     CPATRDPAPKLTEASRAELDWLMRRLERSLEHQRKESVA"
     gene            17239..17745
                     /locus_tag="P5558_09620"
     CDS             17239..17745
                     /locus_tag="P5558_09620"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRY24609.1"
                     /translation="MSSRTEIPLITERVPSPIGDVWLAHDGRALHRLDFGDRLGRGWP
                     AAPAVSVGAVTPSPFARAVAAYFAGELAALDGLAVHVEGRDFETTVWRALRQIACGTT
                     WSYARLAASIGRPTAVRAAGAANGRNPISLVLPCHRVIGADGRLVGYGGGLERKAWLL
                     RHEGVLVA"
     gene            complement(17721..18890)
                     /locus_tag="P5558_09625"
     CDS             complement(17721..18890)
                     /locus_tag="P5558_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRY24610.1"
                     /translation="MALIAAILTMSAMTIDINLPAIPATAQAFGTSQTQAQLSVSLFF
                     LGFAAGQAFFGPLADRFGRRPLLIGGILAYLVATLACALAPSIELLLAARLAQGLAAA
                     SGPILGRAVIRDRFEGPEMARVMSFVMAAFVLAPIIAPSIGALILGVASWRWIFGFLA
                     CYGTVLLVLVVTRLDESLAQRDLRALRPQRIVGAYRAVLGDPVSRRFGALSVLGLAML
                     ISYLISAAPIFMTAYGLTPSAFGVIFAAIAVCSAIGSLLNTRLVRRYPLETLIRLAFA
                     GAVIAMALAFGVAQAGLGGAYALVPLFGLFFLCFSVIVPNATALAMRPHARIAGAATS
                     VLGVAQSVIPAIIGTFVATVHDGTARPAILTMLALALTGLWLARRPAIQATSTPS"
     gene            18961..>19391
                     /locus_tag="P5558_09630"
     CDS             18961..>19391
                     /locus_tag="P5558_09630"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY24611.1"
                     /translation="MSAGEAVGGIYPIVYAFFDGRGRIDRALMQRQVEVCIKGGAHGL
                     ACLGIATEVGKLRPEERQEVLELVATTLAGRVPLAVTVFGRTVEEQAAFVREAAAAGA
                     GWVILQPPVIPDIDEAALLRFFGRVADASPVPVAIQNAPAYL"
BASE COUNT         2792 a         7172 c         6599 g         2828 t
ORIGIN      
        1 gatgcgcctc gtgggcgacg gctggttcac cgccgagaca ccgcttcagc ccggcgatgc
       61 ctatgccttc cggctgtccg aagggagtgt cgtgcccgac ccggcctccc gcgcccagca
      121 gggcggcgtg cacggcccgt ccctcgtgcc ggggccgatc gcgaccgacc cggcgtggcg
      181 tggccggccc tggcacgagg ccgtgctcta cgagctgcat ctgggcacat tcacgcccga
      241 gggcacgctg gcggcggcgg cggcgaagct gccgtacctg gccgacctcg gcatcaccgc
      301 ggtcgagctg atgccgcttg ccgccttcgc cggcagccgc ggctggggct atgacggtgt
      361 cctgccctat gccgtgcacg agagctacgg cacgcccgag gatctggccg ccttcgtcgc
      421 ccgggctcac gcgcatggcc tcatggtctt tctcgacgtc gtctacaacc acttcggccc
      481 ggacgggaac tatctcggcg cctatgccag ccgcttcttc cgcgacgacg tgagcacccc
      541 ctggggggcg gcgatcgact tccgcgagcc ggcggtccgc gcgttcttca tcgagaacgc
      601 gctcttctgg ctcgagacct acggcatcga cggcctcagg ctcgatgccg tgcatgccat
      661 cgtcgacgag ggcgaggcgc atctcctggc cgagctcgcc cgggccgtgc gccagcgctt
      721 cggcggcgag cggcaggtgc acctggtgct cgagaacgag gacaacatcg ccggctggtt
      781 cgacggccgg tatgatgccc agtggaacga cgactggcac cacgtcgccc acgtcatcgc
      841 cacgggcgag acggatggct attaccgtga ctatgccgaa gacccggtcg gcctgctctt
      901 gcgcagcctc gccggcggct tcgtctacca gggcgagccc tcgccgcatc gcgacggcgc
      961 cccgcgcggc gagccgagcg cccatctcca gcccaccgcc ttcgtcgact tcctccagaa
     1021 ccacgaccag atcggcaacc gcgccttcgg cgagcggctg acccggctgg ccccggtcga
     1081 ggccgtggcg gcgctccagg cggccctgct gctcagcccg catgtgccgt tgctcttcat
     1141 gggcgaggag tggggcgcca cgaccccttt cctcttcttc tgcgacttcg agggcccgct
     1201 cgccgatgcc gtgcgcgagg gccggcggag cgagttcggc aagttcgcgg cgttcgccga
     1261 tccggccgtg cgggcgacca tccctgaccc gctcgacctc gccaccttcg agtcgagccg
     1321 cctcgactgg tccgaggggg agcaggccga tcatgccgag cggcgccgcc tcgtccgcga
     1381 cctgctggcc atccgccgcg agcatatcgt gccgggcctt gccggcatgg cgcccggcgc
     1441 caccatcgtc cggcacggca ctcgcggctt cacgctcgcc tggcagctta ccaacggcag
     1501 tgtcctgagc ctgcaggcca atctgggggg cgagcccttg tccggtttcc cgggacttcc
     1561 cggccgctgc ctccatgcca ccccgggcac cgacccatcg gccgatcgtc ggccggcctg
     1621 gtcggtcgcc gccagcatcg accaacgccc gtgagtggcg atctcgacgt ccgtgcccgg
     1681 gcggccggca tcgagcccgg ctactatgac ctcgaaggca actggcatgt cgcctcgccg
     1741 gcgaccaagg cggccttgct cgaggccatg ggcccgctcg acggcgccgc cgagcctccg
     1801 caaggcgcct tcacgcccgc ggtgaccccg gcggcgtgcg ggctcggccg aatctggggc
     1861 gtcgcctgcc agtcctacgg cctgcgctcc ccgcgcaatg ccgggatcgg cgacttcgcc
     1921 gatctggagc ggctcggcac aggtctcgcc acgcgcggcg ccgatctcct gggcctcagc
     1981 ccgctccatg cgcgctttcg cgaccagccg gcgcgagcct gcccctacgc gccgtcgagc
     2041 cggctctggc tcgaccccct ggccatcgcc ctcgacgccg ctgccgccga tctcggcctg
     2101 gagctgccgg ccttgcccgc cccgccaagg ggcgagctcg tcgactaccc ggcggtggca
     2161 gcgctcaagg agcaggcctt cgccgcgctg cacgagcgat tcgccaaggg ccatcccgcc
     2221 ctcgccgatt tccgcgagtg gcggatcgcc aaaggtgcgc ccttggagag tttcgcccgc
     2281 ttcgaggcca ttgccctggc gctccgcgcc cggaccggcc gcccggtcgc ctggcccgac
     2341 tggccggtcg agcttcgccg ggtcgatgct cccggtgtcg ctgcctttgc catcgagcat
     2401 gcgaacgagg tagagcgctc cgccttcctg caatggctgg ccgagcgcca gctcacgagg
     2461 acgcagcagg cgctcatgga tgccggcatg cgcatcggcc tctatgccga catcgcggtc
     2521 ggcgtggtgc cggatggcgc cgatgtctgg gccgaccccg aggcggtcgt ggccggggcg
     2581 acgatcggcg ccccgccgga tgcgttcagc ccgaagggcc agaactggaa cgtcgcgccg
     2641 ctctcacccg tggcgctgcg gcatcgcgac atggcgccct tgcgcgccat tctgacagcc
     2701 gccatgcagc atgccggtgc ggtgcgcatc gaccacgcca tggggctgat gcggctcttc
     2761 tggatcccgg ccggcggcac gcctgccgac ggcgcctatg tgcgctaccc gctccagcac
     2821 atgctggcga cggtggccga ggtgtcgcgc gcccacgcct gcatcgtcat cggcgaggat
     2881 ctgggcaccg tcgcccctgg ctttcgcgag gcgatgctgg aggcggggct cctctcctac
     2941 aaggtcgtct ggttcgagcg ctgggagagc ggtctcttcc gctcctcgaa cctctatccg
     3001 gtagcatcgc tcgccaccgt ctcgacccac gacctcgcca ctgtccgggg ttatctgcag
     3061 ggtcgcgaca tcgactggcg cgaaacgacc cgtcagctcg acgcggcggc ggccgctgcc
     3121 gcccgggacg agcgccgcgc cgactgccag aaactgcgcg atgcgctgcg cgtcgagggc
     3181 ctgctcgatg aggaccagcc ggcggccgac gagcccgagg cgatcgtcct ggcgctgcac
     3241 cgcttccttg cccggacacc gtcggccatc gccatggcct gcctcgagga tctcgacggc
     3301 gccatcgagc agccgaacct gccggccacg atcgacgagc accccaactg gcgtcgccgc
     3361 accattcacg acattgccgg cctgctcggc agcgccccgg cccagcgcct gatccaggcc
     3421 cttgccgagg agcgcccgcg tgattgagac tgtccgcgcc acctaccgca tccagctccg
     3481 cggcggcgtc gatttcgcca ccatcgccag cgccgtcccc tacatcgccc gcctcggcat
     3541 cagccacctc tatctctcgc ccatctggca ggcgcgcgcc ggctcgaccc acggctacga
     3601 cgtggtcgac ccgaacgtca tcgagccagc cctcggcggc gaagccggct ttcgcgacct
     3661 cgtcgccgaa gcgaagtcgg ccgggctcgg catcattctc gaccacgtgc cgaaccacat
     3721 gggcgtcggg tccgacaatg cctggtggtg ggacgtgctg ctgaacggcc gcgccagccg
     3781 ctttgccggg catttcgaca tcgactgggc ggccacggcc ggcgccaccc cgggccggct
     3841 gctcctgccg gtgctgggtg cttcctatgg cgaggtgctg gcctcgggcg accttcgggt
     3901 cgaggcggcc gccggcggct tcgagctgcg ctaccacgac cagcgcttcc cgctccgggc
     3961 cgactcggtc cctaacgggc aggcagagct gcacgcgctg ctcgagcgcc aggcctaccg
     4021 gctcgcctac tggcggctcg gcggcgaggc gctcaactac cggcgcttct tcgatatcga
     4081 cgggctggtg ggcgtgcggg tcgaggacga gaccgtcttc gccgacgccc atcgcctgct
     4141 ccttgagctg gtcgccgagg gtgccgtgca gggtgtgcgg ctcgaccata tcgacggcct
     4201 gcgcgacccc acgggctacc tccatcggct cgacacggcg ctgcgcgagg cgaccggcgg
     4261 cgcgccgccg cccgtctgga tcgagaagat cctcgaaggc ggcgagacgc ttcgccccga
     4321 ctggccggtc gccggcacca ccggctacga ggcggccgat cgcctcgccc ggctcttcgt
     4381 ggccggcgac ggcatgggca cgctcgatgc gctctggcgc caggtcgccg agggcgaggc
     4441 cgacttcgcc gccatgctcc gggactgcaa ggcgctggtc ctcggtgcca gcttcgccgg
     4501 cgagctcgaa tggctggtcg cctcggccct ccacctcgcg gcaggcgatg cccgttttcg
     4561 cgataccggc cgggccagcc tgacgcgcgc cctgaccgct ctaatcctgg ccttccccgt
     4621 ctacaggaca tatctcgacg gcgcatcgcc ggatgccgcc gaccaacagc ggctggcggc
     4681 ggtgctctcc gaggcacgcc agcgcgccgg cctcgccgac gacctgccct tcgccctcgt
     4741 cgagcacatc ctggatgaga cgaccgcgaa ggccgccgcc ctcgactggg ccgcacggct
     4801 gcagcagctc accgggccga tcatggccaa ggccaaggag gacacggcct tctaccgcca
     4861 tatccgcctc gtggcgctca acgaggtcgg tggcgagccg gaccacccgg tgctcgctgc
     4921 cgacgacttc cacgcatttg ccgcagccat gccgccgctc tgtcagctcg ccggcagcac
     4981 ccatgacagc aagcgcggcg aggacctgcg cgcccgcctg atcggcctca gccaccatgc
     5041 cgaggcatgg caggaagccg tcgccggctg gctcgacacg aagcccgatt ccatcgccgc
     5101 gagcgatgcc tgcctcgtct ggcagaccat cgccggcgcc tggccggacg acctcgagcc
     5161 cggcgacgcg cccggccgca aggccttcgc cgaccggctc gacgcctacc tggtcaaggc
     5221 gctgcgcgag gccaaggccc gctcgagctg gaccacgccc gacgaggcct acgagagcgc
     5281 cgtccaggct ttcgctgctt cctgcctcga aggcgcgacc ggccgggcac tccacgattt
     5341 ccgcggccgc ctcctgcccg agggcatcgc ccgcagcctc gcccagacgg cgcttcgcct
     5401 cgccatgccg ggcattccgg atatctacca gggggcggag ctctggaacc tcgggctggt
     5461 cgatccggac aaccgccacc cggccgattt cacgcacctt gcagcgcagc tagccgagcc
     5521 atcccccgac ttcggtcacg actggcgccg cggccgcgtg aagcagttcc tgatccagcg
     5581 cctgctcgcc gatcgcgccg cccggccggc gctctatgcg gagggcgcct atgtgccgct
     5641 cgaagtcggt ggttccgccg tggccttcgc ccgcctggcc gccgatgacg ccctgctcgt
     5701 cgccgtgccg atccgcgccg acccggccct gccgcccggc gaggcaccgc gttttggccc
     5761 gcagttcgcc ggcacccgcg tcgccctgcc gtcggctttg gcggacagga aatggcgttg
     5821 cctgctcacc ggcagggaaa cggacgggcc gctcgatctg ggcgagcttc tggtcgactg
     5881 gccggtcgtc gtgctggcca gtcgttagtc cgccagacat tcggcgaggt tccgggcgag
     5941 gccgagcagc agctcgtgat aggcgcgtgg accgggctcg agcgctacgc cgagcgggtc
     6001 gaggacgccc gttcgcgcct tggtgccctc gatcagcgtg gccaccaggc gtggctcgaa
     6061 ttgcggctcg gtgaagacgc aggcaacctc cagctcgccg agccgctcct gtatttcggc
     6121 aatccgtgcg gcacccggct gcagctccgg gttgacctgg atggcaccgg ccgcctcgat
     6181 gtcgaacgcg tgctcgaaat agtggtaggc gtcgtggaag acgacgaacg gccggccgcg
     6241 gacgggcacc accagcgcct cgacctcggc ctcggtcgta tcgagcgccg tcccgaaggc
     6301 cgcggcattg gccgcgtagc gttcggcgtt ggcgggatcg atctggccga gctcggcggc
     6361 catggcctgc gccatcacgc gggcattggc gggcgacagc cagacatggg agtcgaagcc
     6421 gccatgggcg tgctcgtgcg cctcctcgtg tccgtgcccg gccgccgcgt ggtcctcgaa
     6481 catggcccct tcgcgaaagc ccagccgctc gaccccggcg acctcggcca gcgccagccg
     6541 atgcccgccg gcacccagcg tctccagcgg accggtcagg aacgcctcga aacccgggcc
     6601 gacccagagc accagatccg cttcctccag catcgccgcc tggctcggcc ggaggctgta
     6661 gctgtgcggc gaggcgcccg gctcgagcag cagcgccggc tcgccgacgc cttccatgac
     6721 ggccgcggcg atcccgtgca ccggcaggat cgtcgccacc acccgcggcg gcgtctgggc
     6781 gacggcggcg gcagcgacaa ggaaggttcc gaggaaagca gcgccgcgca tgatggactc
     6841 cgggcgggtg ggccacgtcg ctcgaacggc gggcgcgcat tgtgatcggc ggaggaatga
     6901 cataacataa cattcagccg gcacaagcac cgttcgaacg gagaccatga tggcagcctt
     6961 tccggccgac cgggtcgagc aggcggcatg gatctgcaat gcccgcggcc agaccctgac
     7021 acccttgcgc cgccgggtgt tcgagatcgt gctcgccgcc gaccggccga tcggcgccta
     7081 tgacgtgctc gacatgctcg ccgccgagcg tggccgggtc gcaccgccca ccgtctatcg
     7141 cagcctcgac ttcctcgtcg gccagggcct cgtccacaag gtgctgagcg tcaacggctt
     7201 cgtcgcctgc tcggccgccg cccggcccca cgatgcccag ctcttcatct gcggcagttg
     7261 cggcagcagc gtcgaggtgg cgcgccagct tcgcgccggc ctcatcgagg aggaggccga
     7321 ggcgctcgac ttcgccatcg agagcgtcgt cctcgaggtc catggccgct gccacgcctg
     7381 ccggctggca acggccccat gagccggttg ctcgtcgagg tggtgggcgc ctcggtccgg
     7441 ctcggcgacc atcaggcgct ggagaacgtc gatctcgccc tgcacggggg cgagatcgtc
     7501 agcctgatcg gcccgaacgg cgccggcaag tcgaccctgg tcaagctcgt gctcggcatc
     7561 atcgaggccg attccggcag ggtcacgcgc gcccccggcc tccgcttcgc ctacatgccg
     7621 cagcgcctcg ccatcgaccc gaccctgccg atcgacgtcg accgtttcct cgacctgccg
     7681 caccgccatc cggctgccga gcggcaggca gcgctcgccc gggtcggcat cgccgggctc
     7741 ggccggcggc cgctgcaggc gctctcgggc ggcgagctgc agcgcgtcct gctcgcccgc
     7801 gccctgctcc gccgccccga ccttctcgtg ctcgacgagc cggccgccgg catcgactac
     7861 caggggcagg ccgccttctt cgggctgatc gacagcgtcc gccgggagca gggctgcggc
     7921 atcctcgtgg tcagccacga cctgcacctc gtcatggccg ccaccgatcg cgtcgtctgc
     7981 ctcaaccgcc atgtctgctg cagcggcacg cccgaggcgg tgggcgacga tccgggctat
     8041 ctcgccctct tcggcccccg caccgccgct gcgcttgccc gctaccacca ccatcacgac
     8101 cacgagcacg cgctctccgg cgaggtcgcc catcctaccg attgcgggcg ccgctgatgc
     8161 tgcccgaatt cctcctgcgc ggctggctcg ccggcctggc gctcgccgcc gtcgccggcc
     8221 ccttgggctg cttcgtcgtc tggcgccgca tggcctattt cggcgacacc ctggcccatg
     8281 gtgcgctcct cggcgtcacc ctcggcctcg tcaccggcat cgacctgatg cccgcggtgg
     8341 ccgtcacctc ggcgctgatt gcgctgctcg tcgtcctcat gcagcgccag cgcacgctcg
     8401 ccaccgacac gctgctcggc atcctcgccc attccggcat cgccatcggc ctcgttctgc
     8461 tcggcctcgt cggcgcggtc cgcgtcgatc tcctgggcta cctcttcggc gatgtcctgg
     8521 cggtgcgctg gcaggacgtg gccgtggcgg tgatcggcgg tgccgcggtc ctcgccgtgc
     8581 tccgccacta ctggccgcag ctcctcttcg ccacgctgca cgacgagatg gcccgggccg
     8641 agggcacgcc caccacgcgg ctccggctcg tcctgatgct gctcgtggcg ctcgtcatcg
     8701 ccgccgccat gaagatcgtc ggcatcctgc tgatcacctc gattctcatc atcccgccgg
     8761 ccgccgcccg ccagctcagc gccacgcccg aagccatggc ggtcatcgcg gccggcctcg
     8821 gcgggctcgc cgtcaccctc ggcctcgccc tctcctggca ggccgacacg ccggccggcg
     8881 ccagcatcgt cgtcatggct ctcgccatct tcgtcgcaac acaggcgatc ggacccctcg
     8941 tcgcccgtcg gctgggctgc tagaccgatc cggaacgaca tcggggagga cgagaggcat
     9001 ggcgagcgcc gagcagcgca ttgcggggat caaaccggga cttcgggtct gcgtgacggc
     9061 gggtgccggg ggcatcggcc gggccatcgc cgacggcttc atcgcgcatg gcgcgcgcgt
     9121 cgtggtcagc gatgtcgccg aaggggccct cgccgatttc agcagggccc atcccgacca
     9181 tcatgcggtg gccgccgacg ccggcagcga ggccgcgacc agggccctct tcgggaccgt
     9241 cgaggagcgt ctgggcgggc tcgacctcct cgtcaacaac gccggcatcg ccggcccgac
     9301 ggcgccggtc gaggagatcg ccaccgaggc ctggcaggcc acggtcgaca tcaacctgaa
     9361 cggccatttc tacgcgcttc gcgccgcggt gccgctgctc aagcagacgg aaaacgggtc
     9421 gatcatcatg atcagctcgg tcgccggccg gctcggctac gcctaccggc tcccctatgc
     9481 ggcgaccaaa tgggcgatcg tcggcatggc gaagagcctg tccttggagc tcggctcctt
     9541 cggcatccgg gtcaacgcca tcctgcccgg catcgtcgag gggccgcgca tggacggcgt
     9601 catccgcgac cgcgccgcca cgacgggcgt ctcgttcgag gagatgcgcg agacctatct
     9661 ctcgaagacg gcgctgcacc gcatggtgac cgcccgggac atcgccgaca tggcgctctt
     9721 cctcgcttcg cccatgggcc gcaacgtctc cggccaggcg ctttcggtct gtggcgacgt
     9781 ggtgtcgatc taggggagca ggagatgagt gaaaccgtag ctatcgtcgg caccggcctc
     9841 gtcggccgcg cctgggccat cgccttcgcc cgggccggct ggcagtgccg gctccacgac
     9901 accgcaccgg gtgccgcggc agcggcgcgg gagcgcatcg gcccgctcgt cgacgacctc
     9961 gtcgaggccg ggctgctcga cgacgccgca cccgtgctgg cgcgcatcac cgtcgccgac
    10021 gacctggcca gcgccctcgc cgacgtcgcc tgggtccagg agagcgtgcc cgaggacgtc
    10081 gcgatcaaga aggcgctctg ggccgagctc gaccgcctcg cgcccgccga cgccctcttg
    10141 gtcagctcga cctcggccat cgtgccatcg cgcttcaccg agaacctggc tggccggcat
    10201 cgctgcctcg tcgcccatcc cctgaacccg ccctacgtca tcccggcggc cgagctggtg
    10261 cccgcaccct ggaccgatga cgcggtgatg cagcgcgccc gggcggtgat ggaatcgatc
    10321 ggcatggcac ccatcgtcat gaagaaggag ctcgacggct tcgtcatgaa ccggctgcag
    10381 ggcgccctcc tcgaggaggc gttccggctg gtcgacgagg gctactgctc ggccgaggac
    10441 gtcgatcgcg ggctcaagga cgggctggcg ctccgctggt ccttcatggg gccgttcgag
    10501 accatcgacc tcaacgcgcc cggcggcgtc gccgactatt gccggcgcta cgagcagatc
    10561 taccagcgcc tgcagccgac catgctgcat cgcgccgact ggaacgggcc ggtgctggaa
    10621 cggatcgtgg ccgaacgccg ggcgaaggtg ccgcaaacgg cgctcgcaga ccgacagcgc
    10681 tggcgcgacc gccggctgat ggcgctgacc gcccacaaga gaaatgccgc cagggacatt
    10741 ggagactgac atggctggac ccgagaaggt catcatcacc tgcgccgtga cgggcgccat
    10801 ccacaccccg tcgatgtcgc cgcatctgcc ggtgacggcg accgagatcg ccgatgccgc
    10861 gatcggcgcc gccgaggcgg gggccgccat cgttcacctc cacgcccgcg acccggagac
    10921 cggccggccg gaccagacgc cggaggcctt cagccccttc ctccaggtca tcaagcagcg
    10981 ctcggactgc gtgatcaaca tcacgaccgg cggcggcccg gcgatgacca tcgaggagcg
    11041 ggtcaagccc gccgccacct tcgcgcccga ggtcgcgtcc ttgaacatgg gctcgatgaa
    11101 cttcggcctc tttcccatgc tgaaccgctt caagaccttc aagcacgact gggagcgcca
    11161 gcagctcgag agcagccgca gctacgtctt caagaacacc ttcgccgaca tcgagtacat
    11221 cctgacgacc tgcgccgaga acaacacgcg cttcgagttc gagtgctacg acatcagcca
    11281 cctctacaac ctcgcccact tcctggaccg cggcatcgtc aagacgccgc tgttcgtcca
    11341 gtcggtcttc ggcatcctcg gcggcatcgg cccgcacccc gaggatgtgc tgcacatgaa
    11401 gcgcaccgcc gaccggctct tcggcacgag ctatcgctgg tcggtcctcg gcgccggccg
    11461 caaccagctc ccgatcgccg ccatgtccgc cgccatgggt gccaatgtgc gggtcgggct
    11521 cgaggattcg ctctggctcg gcaagggccg gctcgccgag agcaacgccg cccaggtcaa
    11581 ggcggcccgc cagatcatcg agggcctcgg gcgcgaggtg gccacacccg acgaggcgcg
    11641 ggagatcctc cagctcaagg gcggggacca ggtgaacttc tgatggcggt ggccggcccg
    11701 gcccaggcga ggccgggcgt cggcatcttc tggatgtgcc tctcggcctt cggctcgtcg
    11761 tcgatgaacg gcatcgtccg cgaggcgacg actgagatgc acgccttcga ggtggccttc
    11821 ttccgcaacg tcttcgggct cgtggctttg gcgccgatga tgctcaaggc ggggctgcgc
    11881 gccacgctgc gcaccaagcg gctgcatctg cacgcggccc gcggcctgct caacgccgtg
    11941 gccatgctct ccttcttcta cgccgtcacg gtgacgccgc tcgccaccat cgccgccctg
    12001 gctttcacgg ccccgctctt cgcggccctc ctcgccatcc cgttcctcgg cgagcggccg
    12061 ggctggcggc gctgggccgg gctgatcgtc ggcttcgccg gggcgctgct catcatccgc
    12121 cccggcttca ccgatgtcgg catcggcgtg ctcctgatcc tgctctcctc gctggtctgg
    12181 tcgggagcgc tgatcatcat caagatcctg gcgcgcaccg agacgagcct gacgatcacc
    12241 acctatgccg ggctcttcct gacgcccatc accggcgccg ccgcctgggc cttctggatc
    12301 tggcccgatg ccaccggctg gctcatgatg atcgccatcg gcatcctcgg cagcatcacc
    12361 cagtggtcgg tcgcccaggc cttccacgag gccgacgcca cggtcgtcct gcccttcgat
    12421 ttcaccaagc tcatctgggc ctcggtcatc ggctatgtct tctttggcga gaggcccgat
    12481 cccatgacgc tcgtcggcgg cttcgtgatc ctcgcctcga tcacctatgt cgcttaccgc
    12541 gagcgcgaga tccgatcgtc ctgaagctca gaatcgaagc cagctccgca gcttcaccac
    12601 cagccgctcg tccgggcgga gcgccgcccg ctcggcgagg agtgacctga gctcgcccgg
    12661 caggtcggtg atgatcacgt cgacgcccat gtcgatcagc cgcgacatct cgcgccggtc
    12721 gttgaccgtc caggcgtgta cctcgtagcc gagccgatgc gcccggacca cctgctcggg
    12781 cgtgagcgcg ctggcccgga tcgagaggat gtcgtagtcg cgtcgatccg gctcgccgat
    12841 cgtcgagtac acgaggagcg ctcgccgaag tcgcggctcg agcacgccga cctcgtccag
    12901 caccgcccgg tcgaacgagg cgacgatggt cccctcgatc gcgtccaggg cctgcaggcg
    12961 cgccaccacg ttgcccacga ggcccggcat ctggggggcc gtcttgatct cgatatagag
    13021 cttggcctcg ccgcccaccg ccaggaccgc cgcatccagg gtcggggccg gcacgcccgc
    13081 gaagctcgca tcgaaccagc cgccgacgtc gatggcgctc agctccgccg ccgacaggtc
    13141 ccagatgcgc cggtcgatgc tggccacgcg gcgcagatcc cgatcgtgca gcagcaccgg
    13201 cacgccgtcg accgtctcct gcacgtcgac ctcgacatag tcggccccgt cgtcgacggc
    13261 gcggcggatc gccggcagcg tgttctccgg cgcggccatc gagctgccgc gatgcgccgc
    13321 gatgcccacc tcgtcggtga ggtcgaagcc ggccaggacg atcgccgcct ggacgaagac
    13381 gaagccgagc agcgccagct cgaccgccca caaggcgagc cgcccgccgc cctgcggcat
    13441 cgccgcgagc gccggccggc cggtgccgct gagccggcgg tgcagggcct gcaggacgag
    13501 cgcgttggcg gcgatgccga gatatgtcgc ggcgatggcg ccgaagatcg ccagcgcgat
    13561 gacgaagagg gtcgctggga ccaccagcgc catgcggtgt ggcaggttgc cgaggagcag
    13621 agccgccgtc cggtcgacca gaaaagcgga gagcagcggc aagagcaggg cggccaccgt
    13681 caccggcagc acgacgagga tcgtccgtgc gagccggccc cgggtgagcg cggcactccg
    13741 gcgaagggcg gaaagggcgc ccaaattctc cagaagcacc gccggcgggg ccagggacca
    13801 gcgcaggtag aggaacgcgt tcgccaggac gagccccagc gccagcggtg ccgccatggc
    13861 gaggaagggc cagatgaccg gcggccggac ggtccagagg aaatagatgt cgtagtcgcc
    13921 gagccgccag acgaagagcc aggcaatgcc agcgacgaag ggcagggtca cggccgcctg
    13981 gatcgccacc cgcacgaagg tcaggcccag gagccggggt gcgaggcgca ccacatgcca
    14041 gagcgccgcg gttgccgcgc gatagcgccc gcgcggcgcc gccgccgcga tcacgatcat
    14101 gcccgcctcg tcgagcgtga tcagcgtgac cgtcacggtg gcggcggcga gcgcccagag
    14161 cagcccccag ggcgagagca ggaaatcgag gatctcggcg ttggtcatcg ccggctcgcc
    14221 gctcgtcgtc aaaagcgcgg tgagcaccag ggacgtcgtc ggcaccaggg ccacgaccgt
    14281 caggatcgtg aagaaaaggt ggtagaggag gagcgagcgg ccgtagcgca cgaagccgac
    14341 ggacgcctcg ccgaggatct tgggggcctt tctcatcctg ctttcggctc ggccacgggc
    14401 ggggccagca tcaggcggcg ggtcgcctcg tcgaccacgg gccgcgtgcc gccccaatgc
    14461 gtgaagcccg gcaccgcctg gtgcagcagc atgccgagcc cgtccaccgt ccgatggccg
    14521 cggcgccggg cctcggagag cagcggcgtg gagagcggcg tgtagacgat gtcggcgaca
    14581 atggccgtgg ccggcagggc agcgagatcg agatcgagcg ccggctggcc gaccatgccg
    14641 aggctcgtgg tgttgaccag gagatcggcg ccttccaggg cgcggtgccg ctccgcccag
    14701 ggaagtgcca ggacggcggc atcgttctgg tcgcccagcc gggcgaccag cgccgcggcc
    14761 cgctccggtg tgcggttgac cagcgccagc tcatggattc cggcggcgag gaaggtgaag
    14821 gcgagggccc gggcagcgcc cccggcaccg agcagcacca cccgccgcgt ggctgcccgc
    14881 cagtccggcg cgcagccgtc gagatgagcg aggaagccca gcccgtcggt gttgtagccg
    14941 gtgaggctgc ccgcatcccc gaccaggacg gtgttgacgg caccgatcgc ggctgccagt
    15001 ggatcgaccg cgtcgaccag atcgagcatc gccgccttgt gcggcagggt gacgttgcag
    15061 ccgagaaacc ccatcctcgg cagggcgcgc atgaccggcg cgagatcctc gggcgccacc
    15121 ggcaagggca cgtagacgcc atcgagcccg tgccgctcga accagaaatt gtgcaggagc
    15181 ggcgatttcg aatgacccac cggccagccg agcacgccgg cgagcctcgc cttgccgctg
    15241 atcgtcacca acgtccggga tccctctcgt gagcttcggg gggcggctcg cggacgcctt
    15301 ctggcgcaac gcctaccgcc atggctatcc ctgcgcgcgg ctcgtctggc gcctgaccaa
    15361 cccgagcacg cggggcgtca gcgtcgccat ccggcatggc gatcgcctcc tctgcatccg
    15421 cgagtcctac cggcccggcc tcagcttgcc gggcggcggc atcgatcgag gcgagacccc
    15481 gctcgaggcc gcgcagcgcg aggcgcgcga ggaggtcggg ctcgaccttg cggccgaggc
    15541 cttcgtcgag ctcggccgcc tcgacttcaa cgcggacgac cggacccatt tggtcacctt
    15601 cttcgcgctc gatctgccgt cgatgccggc acccaccgtc gaccggcgcg agatcgtctg
    15661 ggcgggctgg ttgaccaggc aggagctcgc cgacgcggaa cgccagcaca ccatcgatct
    15721 ctatgtcagg cactttgccg gggacctctg atcaggcagg cccgacgtcg agcggcgtcg
    15781 ccagctccgc ccaggcggta tcgaccgaag cgtcgcccgt cgccgccatc atcatgagaa
    15841 acgtgctggc gtggtccagg atggtgatcg gcaggtgcca gaccccccgc cggtagcaga
    15901 tgccggtgcc gccgctgacg agaaagccct tgagcgtggc gagatcgggg gccccgtccg
    15961 ggcccggcag ggcggcgccg atggcgtagc gggcggggcc gatcggcagg aagagctgct
    16021 cggtcagggg gtggctctcc atcctgcgcg cctgcatcgg cagcaccccc gccggatggg
    16081 tgtcgagctt gacgcagatg gcggttcccg gcccgccctc gccgagatgc gccaggagat
    16141 cggcattgcc cttcggcgcc ggcggctcga ccacgctgcc gaaagccgcg aaggtcgccg
    16201 ggtcgaacgc ctgcagtgcg agcttcatgc cacgctttcc ttccgttgat gttcaagaga
    16261 gcgctcgagc cgccgcatca gccagtcgag ctcggctctc gaagcttcgg tgagcttcgg
    16321 cgccgggtcg cgggtggccg ggcaggcgag ggcgccgcgt cgccagagca cgtatttgcg
    16381 caccgcgagg ccgaggcccg gctgctgctc gtagcggacc agcggcaggt agcgatcgaa
    16441 gagatcgagc atcgcctcgc tctcgccccg cgcaaagagg cggcagacct cgaccagcat
    16501 ctcgggatag gcgaagccgg tcatcgcccc gtcggccccc cgtgccagct cgaacggcag
    16561 gaagatgccg ccattgccgg tgaggatcga gacccgcctc gccccttccg cctcggcgcc
    16621 gcgaagatcg ctcagcttgt cgagcccggg ccagtcctcg tgcttgagca tgaccgcgtt
    16681 ggggcaggcc gccacgatcc gctggatcaa cagggcgctc atcggcacgg atgccacgag
    16741 cgggaaatcc tgcagcaccc agggtgtcgc ctcgcccagc gtctccgcca ccatccggca
    16801 atagccgagc accgcgtcgt cgcctctggc agaaactgcc ggcggctgca ccatgagacc
    16861 ggcggcaccg gcatccatca ccacgctgcc cagcgcccgc atcgaagcga agcccggggc
    16921 cgagaccccg accactaccg gcacccgagc gtcgacccgg ccgagcacat ggagggcgaa
    16981 cgtccgcgcc tcctcctcgg tcagcttcgg cgcctcgccc atcatgccga ggatggtcat
    17041 gccatccacg ccctgctcga gatagaactc gatcaggcgg tccgtggcgg cgaggtcgag
    17101 ccggccgtcc tcggtgaacg gcgtggcggc gatgatgaag acccccctgg cggaggcgtc
    17161 gaggcgtggc atgaccattc ccgttggctg gtgcgcggct ttctagcgcc tcgcaccggg
    17221 gctggccata atggcggcat gtccagccgc accgaaatcc ctctgatcac cgaacgcgtg
    17281 ccgtcgccga tcggcgacgt ctggctggcc catgacggcc gcgcgctgca tcgcctcgat
    17341 ttcggcgacc ggctgggcag aggctggccc gcagcgccgg ccgtttcggt cggtgccgtg
    17401 acgccgtcgc ccttcgcccg cgccgtcgcc gcctatttcg cgggcgagct cgccgctctg
    17461 gacgggcttg cggtgcatgt cgagggccgc gacttcgaga ccaccgtctg gcgggcgctg
    17521 cggcaaatcg cctgcggcac cacctggtcc tatgcccgcc tcgccgcgtc gatcggccgg
    17581 ccgacggcgg tgcgcgccgc cggtgccgcc aacggtcgca acccgatctc gctggtcctg
    17641 ccctgccacc gggtgatcgg cgccgatggc cggctggtcg gctacggcgg cgggctcgag
    17701 cgcaaggcct ggctcttgcg tcacgaaggc gtgctggtcg cctgaatggc cggccggcgg
    17761 gccagccaga ggccggtcag ggcgagggcc agcatggtca ggatcgccgg ccgcgccgtg
    17821 ccgtcgtgga ccgtcgccac gaaggtgccg atgatcgccg ggatgacgct ctgcgccacg
    17881 ccgaggaccg aggtcgccgc accggcgatg cgggcgtgcg gccgcatcgc gagcgctgtg
    17941 gcattgggca cgatcacgct gaagcagagg aagaaaagac cgaaaagcgg caccagggca
    18001 taggccccgc cgagcccggc ctgcgccacg ccgaaggcca gcgccatggc gatcacagcc
    18061 ccggcgaagg cgaggcggat gagcgtctcg agcgggtagc gccggaccag ccgggtattg
    18121 agcaggctgc cgatcgccga gcagacggcg atcgccgcga agatcacgcc gaaggccgat
    18181 ggcgtcaggc cataggctgt catgaagatg ggtgctgcgc tgatcaggta gctgatcagc
    18241 atggcgaggc cgagcacgga aagggcgccg aagcggcgtg aaacgggatc gccgaggacg
    18301 gcccggtagg cgccgacgat acgctgcggc cggagcgccc tgagatcccg ctgcgccagg
    18361 ctctcgtcga gcctcgtgac cacgagcacc agcagcacgg tgccgtagca ggcgagaaag
    18421 ccgaagatcc agcgccatga ggcgacgccg aggatcaagg caccgatcga gggcgcgatg
    18481 atcggggcca ggacgaacgc cgccatgacg aacgacatga cccgcgccat ctccggtccc
    18541 tcgaagcggt cgcgaatgac cgcccggccg aggatcggcc cggaggctgc cgccaggccc
    18601 tgcgccagcc gtgctgccag cagcagctcg atgctgggcg cgagcgcaca ggcgagcgtc
    18661 gccaccagat aggcgagaat gccaccgatc agcagcggcc ggcggccgaa gcggtcggcc
    18721 agcggcccga agaaggcctg gcccgcggcg aagccgagga agaagagcga gacgctgagc
    18781 tgcgcctggg tctgcgacgt gccgaacgcc tgcgcggtcg ccgggatggc cggaaggttg
    18841 atgtcgatcg tcatcgccga catggtgagg atcgcggcga tcagtgccac aaaggaagcg
    18901 gatcccggca cgagggggat tttcggcatg gctgcccaag gtggcattag gagagagaag
    18961 atgtctgctg gcgaggctgt agggggtatc tatcccatcg tctatgcgtt cttcgatggg
    19021 cgtggccgga tcgaccgggc gctgatgcag cgccaggtcg aggtctgcat caagggcggg
    19081 gcccacggcc tcgcctgcct cggcatcgcc accgaggtcg gcaagctgcg cccggaggag
    19141 cggcaggagg tgctggagct ggtggcgacc acgctcgccg gccgggtccc gctcgccgtc
    19201 accgtcttcg gccgcaccgt ggaggagcag gccgccttcg tgcgcgaggc cgccgccgcc
    19261 ggggccggct gggtcatcct ccagccgccg gtcattccgg acatcgacga ggcggcgctg
    19321 ctgcgcttct tcggccgggt cgccgatgcc tcgccggtgc cggtcgccat ccagaacgcg
    19381 ccggcctatc t
//
LOCUS       DAOTFI010000076        19341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3394_length_19341_cov_6.215960, whole genome shotgun sequence.
ACCESSION   DAOTFI010000076 DAOTFI010000000
VERSION     DAOTFI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19341
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3394_length_19341_cov_6.215960"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..490)
                     /locus_tag="P5558_09635"
     CDS             complement(173..490)
                     /locus_tag="P5558_09635"
                     /inference="COORDINATES: protein motif:HMM:NF017174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HRY24612.1"
                     /translation="MPEYAWVLGVRPGYEDEYKKRHAEIWPEMEAALKAAGITSYAIF
                     RHGLTLFGYFETADIDATIAALKASETNRRWAEYMAPIMDIQIDPNTGFPYLLPKQWT
                     MRR"
     gene            complement(504..1550)
                     /locus_tag="P5558_09640"
     CDS             complement(504..1550)
                     /locus_tag="P5558_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY24613.1"
                     /translation="MTKKARIAVIGAGWWACHNYLPALLAHPDADLVAVNRPDKEALD
                     RVTQHFGIARGYTDTARMLAEERPDAVVVASPHVLHFEHAMAGLEAGAHVLVDKPMTT
                     SAADARTLVAKAQAVGRELVVPYGWNFQPYTTRAHELVREGAIGRIEHVVLQMASPLD
                     DLMAGEPMAETRDHLFRPPASTWADPKRAGGYGWGQLSHALGLLFRVTDLEPRDAYAA
                     CVRSKAGVDYHLAATLNFKNGATAAVSGSSAVPKQCGFQLDLRLFGTEGMLLFDVERE
                     RLEARRRDGRDTVVPIEPGAGAYTCTEPVNRLVQICLGTAVTNEAPGVVGCRAVEALD
                     AIYRSFGSGRSEPV"
     gene            complement(1547..2527)
                     /locus_tag="P5558_09645"
     CDS             complement(1547..2527)
                     /locus_tag="P5558_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24614.1"
                     /translation="MEGGLMRRLVRWEVLLLVILAVTIAVNSAMAPMFLTIGNQINLF
                     QLSIEKIIVALMMTLVIVNGEIDLSVASMMGLAACAFGWLFQMGVPAGVAIPIVLALG
                     LLGGAFNAFWITVIGMPSLVVTLAMLIGFRGMARILVEDRGITGFPDWFNALGQQPIL
                     GPLPLALLVFAVLFVVLLVVLHRSGFGRKVYVIGNSAEVARYSGVAVPRVKALLFVAS
                     GFVAALAGLLYTARVSAVRGDVALGFELDIITMVLLGGVSIFGGKGTLWGVLLSILIV
                     LNLRNGMALANITGHLQTGVIGLLLILSVLVPNLVQSAGRLSFKPGSASR"
     gene            complement(2527..3579)
                     /locus_tag="P5558_09650"
     CDS             complement(2527..3579)
                     /locus_tag="P5558_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24615.1"
                     /translation="MTGWLRSVHPQTLRVTALALVLALVVLFFGSQIDNYYTARMFNR
                     ISTSVAIILLIAIGQALVVITRNIDLSVGSIVGFTAYLVGDLLVAQPDLHPLVVVAAA
                     MALGAALGAVNGVVVAYGRVPAIIVTLGTLAIYRTILVEYSDARSITTANLPKWVVEL
                     PQATLFSIGDLDIRVTVAIALGVALVVHLALTRLRAARRLYAVGSNPDAAAIAGINAE
                     RVVFTAFLLSGALAGLAGFVFLARFGNITVVAGLGFELKSVAAVVVGGVNIFGGSGTV
                     VGVVLGTTLVDVIDNSLTRWALVSEFWRDALLGMLILIAVAADTLMNRYLDRLRRKRH
                     MAELSAGTLPVPREAG"
     gene            complement(3576..5081)
                     /locus_tag="P5558_09655"
     CDS             complement(3576..5081)
                     /locus_tag="P5558_09655"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY24616.1"
                     /translation="MPEPVLALDGIAKSFALTAALADVSLELLPGEIHALVGENGAGK
                     STLIKIMTGVHQPDAGTIRLAGTAVTIADTQAAQRLGIAAIYQEPMVFPDLDVAENIF
                     ISHRDLGLWMDWRRLHEAARREIERLGVTLDVRRAASGLTLAEQQTVEIARAISLDVK
                     VLIMDEPTASLSAHEVARLMAIARTLKGQGVAVLYITHRLEEVFELADRVTVLRDGRH
                     ISTKPIAEVTPDALVRDMVGRAVDHFVPKAAAGPHGERVLRVRGLAREGVFTGVGFDL
                     HAGEILCFSGLVGSRRTDIGLALFGIQPATSGSIELDGEAAQIESPKAAMGHGIAYVS
                     EDRRKLGLAMALPISANISLPSLRRLLGKLGLIDRRAELAMAEKHRERLNIRTHSVAL
                     EVGKLSGGNQQKVMLAKWLETEPRLLIFDEPTRGIDVGAKTEVHAIINDLAARGVAII
                     VISSDLPEVMALADRVLVMREGRQMGIFAIEDATPEGIMSLATGERPAAAA"
     gene            complement(5118..6170)
                     /locus_tag="P5558_09660"
     CDS             complement(5118..6170)
                     /locus_tag="P5558_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY24617.1"
                     /translation="MKFAGSSCLAATALLMGSSLMGPTALAEPVTATKGESVDMVLLP
                     KFLGILPFDQAHRGAQEAHEELENKGELLYVGPTPENSVAGQIEIMTTAATQGQDVVM
                     LSNNSGDQIVPSAQAAQEAGTRVVTWDSPIPSAEGETVFVAQVDFNDIGVVMADMAHN
                     IMGGEGEFAILSATPDAANQNAWIAAMQRALEGEKYANMTLVDTVYGDDQSELSYNRA
                     LALVDKHANLKLIMAPTSVGIVAAAKAMQDEGLCDQIKISGLGVPAEMVSYTMNGCAP
                     EFALWSFVDLGYLTYYTSYLIATGAIAGEVGDEFEAGRMGTFSIEKDPTRDAGNRVLM
                     GPFTVYNADNVEAAAQ"
     gene            6332..7171
                     /locus_tag="P5558_09665"
     CDS             6332..7171
                     /locus_tag="P5558_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY24618.1"
                     /translation="MQQLGALSLTSWSLTHCTLGEAANIGRALGIEGLDLGLLHGPAL
                     AKGRLLADPEALAGEIRRLGIICPNLYWLFGNGLVDRNLADPEARAANEADFRRVLAF
                     ATAASIPTIFVLPGVCGRGIGREAALERSAASLRPLAAMAGDAGVTLTVEPHVGGFLE
                     SPGLTLALLDAVPGLKLTLDYAHFACLGFTQGEIDPLAPHAAHVHLRQARPGALQCKL
                     EHGTLNFPAMLATLAGHGYRGRLAIEYVHQGYMDTLHDDVLSETIKMRDLVRLQTASP
                     AAR"
     gene            7807..8544
                     /locus_tag="P5558_09670"
     CDS             7807..8544
                     /locus_tag="P5558_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRY24619.1"
                     /translation="MPDPAPDLARPRIGITLDSEEPGGYSKFPWYALRQNYGSAVLAA
                     GGLPFALPHVPRAQVDAYLDQLDGLIVTGGAFDIDPALYGAVERHPTVDLKAARTAFE
                     WAITERALARDLPVLGICGGQQLLNVVLGGTLIQHIPDVVPDALAHEQPNPRNEASHV
                     VDIVPGSLLHRICRHDVLAVNSAHHQAADRTGRGVVVSATAPDGVVEAIEHPGYRFCL
                     GVQWHPEFLISEGDLPILQALVRAAAR"
     gene            8541..9392
                     /locus_tag="P5558_09675"
     CDS             8541..9392
                     /locus_tag="P5558_09675"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRY24620.1"
                     /translation="MSEAPDGTRPAGARLPGERIAKRIARAGYCSRRDAEKLIQEGRV
                     SIDGRTVESPALNVTAGQEIRIDGERLKAAEPARLFRYHKPAGVVTTAHDPEGRPTVT
                     DKLRVRTADGSLPRLMPVGRLDFSSEGLLLLTNDGGLKRLLELPATGWLRRYRVRVYG
                     NVDRNALESLAAGIEIDGIRYGPIKAEEESRTGANAWLKVGLREGKNREVRRVLGHLG
                     LEVSRLIRTAYGPFQLGKLGRGDVEEVTPKVLKEQLGGLGAGLALPESKSHSHERRLT
                     RPVAGPA"
     gene            9389..9952
                     /gene="rsmD"
                     /locus_tag="P5558_09680"
     CDS             9389..9952
                     /gene="rsmD"
                     /locus_tag="P5558_09680"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRY24621.1"
                     /translation="MSPPRIIAGRHRGRRLEVPAGDIVRPSAERLREAVFSMLEHGQP
                     RLRDCRFLDLFAGSGAIGLEALSRGADEACLVEADRRAAGAARRNIEKLGETGRARLV
                     VADATRLPPAGEPFAIAYLDPPYGSGLVPACLACLAAGGWLVAGALVVVEIAAREALA
                     PPQGFDSLDERRYGAGRIVRLRFAPGN"
     gene            10033..11295
                     /locus_tag="P5558_09685"
     CDS             10033..11295
                     /locus_tag="P5558_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY24622.1"
                     /translation="MRVLTLLLFMLAIFLINAHFIRVGEEENRIWPPMVLALLSLILL
                     LRTPNLLRVNWKLLVSPVVLLFALNLVWLGIVQFWSVAPAGGRSHIILLWLTLVAALA
                     LSEERPYRSVLAFVGCLCIVYAWSWAGAAAGQDWVVLDRSQWRLKGVMMHPQTLALVA
                     ITGVIVGLVWQLNRQTAGAVPHRGRMTAFFLVSLATLLATQGRSLTAFFVVTLFVVYF
                     FHIKGPKRIWVLLAGIAIGIALYVAVDIILPMISRGQEETLSGRTIIWEMTLREIAKA
                     PFLGFGFGTYQDYFSSFYNNWSPGHAHNLWLQVTFESGLVGTVLYTFFLLALMQRGYR
                     FQRDTGLVSYTLCLTIFCLLGGMTSVFLGEKLSTLYGLLLLLALQEERLRLEALARQR
                     VGTLPLHRPSASPSGTEARPPGAEPQPA"
     gene            11626..13620
                     /locus_tag="P5558_09690"
     CDS             11626..13620
                     /locus_tag="P5558_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY24623.1"
                     /translation="MTGLDDQLAGAAGSLAGMAYLGLALLLGRRPRAPASAPILPLVI
                     AVLAGAFAGFLAGVVGPVVARPAGFVETLCWIAFLAFLGHALADSAGRWLAAAILPLA
                     AAMASMAPFDPWLMATGGAAVIGLFLVLAQGLARAPGEAGRRLLLLGLGVGFTFDLVI
                     AGLALADAPLLGAAEAARPLLRAALVPLIAMATSPVLARPGRIVPSTTLRRLALLVLA
                     TTLALLAVAAGAGWLGTRLGAALALALGLPAGLFVLAAGLAPGAGSRLDNLLRRHLPV
                     HRHDYRQVWPAFVDVLTGADPRPSDDLPERVIRAVAGTVGAAGGGIWLAEPGDRFRRL
                     ASLALPSPAETAGRAPALAAALLAAEGPLSMADPLPGGGQWPAFLPRPAAAWLALPLI
                     HKEALFGLLVLAGPSGRPHLDDEGRELLRLVARQAASYLAEDETARQLADARQFESFT
                     RRFAYVMHDLKNVGSELALTLANARRHRDNPAFYDDLLETLEASVDRLGRLIAQLRNE
                     RQPRLEPVALAPLLRRLVQSRADRRLALAEPLPSLAARVEADALEMALDHLIDNALAA
                     LDAGGRVTLGLVRRDRMATVLVEDDGPGLPPGLVDAAGRGRPFTSCRPGGLGLGIDQA
                     RNTVERVGGRFELVSRPGRGTAVALHLPVLEEAAGEEVAA"
     gene            13617..14966
                     /gene="prsR"
                     /locus_tag="P5558_09695"
     CDS             13617..14966
                     /gene="prsR"
                     /locus_tag="P5558_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HRY24624.1"
                     /translation="MTERRLLVVEDDVALQKQLRWALDAFAVLPATGREQALAMLRAE
                     EPPVVLLDLGLPPDPCGVSEGFATLRQVLDLVPSTKIVVMTGQEGRQHARDAIAAGAH
                     DFHQKPVDPDQLGLLLARAFYVGELEAENERLQARARQSRMPGIVGDSPAMLKLYRAL
                     ERSGPTDVSVALLGESGTGKELVAHALHSLSPRRDRRFVAINCAAIPPTLLEAELFGY
                     ERGAFTGAFRQTPGKIELADRGTLFLDEIGDLPLDLQAKLLRFLQERVVERLGGRQEI
                     AIDVRVVSATHRKLEDLVARGGFREDLYYRLAELSLEIPPLRDRAGDALLLARHFLSR
                     FGPELGKQRIGVSAEALAAIDAYAWPGNVRELQNRLKRAIIMCEGRRIGRADLDLAAE
                     DEADAVLNLRQERERAELGALRRALARAEGNISRAARLLGVSRPTLYDLLRQHGLRD"
     gene            complement(14978..15361)
                     /locus_tag="P5558_09700"
     CDS             complement(14978..15361)
                     /locus_tag="P5558_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24625.1"
                     /translation="MCHGEPPAQAASVPVPGWHRVDAGDAATATATKKRARPDYPGGP
                     LKFGTMRSPRLEADVGSATTIAFATTTGSLAVRVMSGGCKARDAGFNNTIMWCMTVRK
                     ASRKTAGNWQQYQNTVDFIYTIHLI"
     gene            15425..16852
                     /locus_tag="P5558_09705"
     CDS             15425..16852
                     /locus_tag="P5558_09705"
                     /inference="COORDINATES: protein motif:HMM:NF033792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HRY24626.1"
                     /translation="MQDRSASRPAAAPTGGPPPLVVSPGPRPAAGGRPERWWRRLGRL
                     GTTGEIGVRFFTLAVLRWMALVGQTFTILVVHFSLGIELPMLWLVPALLLTLAVNVGV
                     QVRGTPTARLGARQTFFLTLFDVLQLTFLLFVTGGVQNPFTVLLVLPVGLAAATLDRA
                     PTAILTAVALAAIGFMAAIPMELPWLDGGLRLPALYLFAAWLALSLTVVLVAVYVCQL
                     ASEARRHALALSALQLALAREQELSALGGQAAAAAHLLGTPLATINVIAKELVRELPA
                     TSPLLEDASELLLQARRCRETLASLGSGQRDAAHAAFTRAPLSGLLATIAETCRIEPV
                     TIDIVVDVEPGLEEPRVVLAPELRHSIANLVDNAVRFARTRVDITVAVAHAGIAVEIA
                     DDGPGFTPEVLDWLGEPYLSTRRAEGGLGLGVFIAKTLLARTGASMHFANRDKLSHRE
                     EGAVVRIVWPSGALEMLGRESADDG"
     gene            16920..17432
                     /locus_tag="P5558_09710"
     CDS             16920..17432
                     /locus_tag="P5558_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HRY24627.1"
                     /translation="MLVDDDAPLRRSLQRAMERRGFQVYAGESLKEGLNIAHSVKPAY
                     AVIDLRLEDGSGIDLVARIRELHPAIRIVVLTGYGNIATAVAAIKAGAVDYLAKPADA
                     DDVIAALLATGSGLPPPPANPMSADRVRWEHIQRVFEQCNRNVSETARRLNMHRRTLQ
                     RILNKRAPKE"
     gene            17533..18099
                     /locus_tag="P5558_09715"
     CDS             17533..18099
                     /locus_tag="P5558_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY24628.1"
                     /translation="MIAGADSKSAAPAEPTAKKLLLVDDDAPLRRNLVRSLERFGFVV
                     ATAASVREAYEVASEFLPELAVVDLNLEDGHGMELVESLQELRPGLRVVVMTGYDSIA
                     SSIVATKAGAVGYLAKPVKPEELVAVLDGSAEPAVDDSPMSADRVRWEHIQRVFEQCN
                     RNVSETARRLNMHRRTLQRILSKRAPRP"
     gene            18110..>19341
                     /locus_tag="P5558_09720"
     CDS             18110..>19341
                     /locus_tag="P5558_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRY24629.1"
                     /translation="MSRPIEDYALIGDTRTAALVGKDGSIDWLCVPRFDSPACFAALL
                     GDEGNGHWRIAPIDATATVRRHYRDATLVLETEFTTDAGAVAVVDFMPQPRNDGQVDV
                     VRLVQGRRGAVDMCTELVLRFDYGSVVPWVERRGYGLRAVAGPDAVRFESPVALENEN
                     FRSIGRFTVREGETWPFRLTWFPSHRDGPVVGEWQAELERCEAWWHDWAERCTYEGPY
                     RDAVLRSLIVLKALTYGPTGGIVAAVTTSLPERVGGARNWDYRYCWIRDSTLTLDALA
                     SSGFLAEARAWRDWLLRAAAGKPSQLQIMYSVTGERRLQEAELPWLPGYEGSRPVRIG
                     NAAHGQVQLDVYGEIMDSFHIDRVHGMPASDAAWQLQRVLLEALETAWRQPDEGIWEV
                     RGPPRHFTHSKVMAWVAF"
BASE COUNT         2783 a         6930 c         6571 g         3057 t
ORIGIN      
        1 gcgctaccag gaagcctaca acgccgagat ccaggccttc gtcgacgccg tcgaggccgg
       61 cgcacccatg cccgtcggct tcgacgacgg caggaaagcc ctcctcctcg ccaacgccgc
      121 ctaccgctcg ctggcgagcg gccgggcgga gacggtggag gcttaggggc gcctaccgcc
      181 gcatcgtcca ctgcttcggc agcagatacg gaaaccccgt attcggatcg atctggatgt
      241 ccatgatcgg cgccatgtat tcggcccagc ggcggttggt ctcgctcgcc ttgagcgcgg
      301 ctatggtggc gtctatatcg gccgtctcga aatagccgaa gagggtgagg ccgtggcgga
      361 agatggcgta gctggtgatg ccggccgcct tcagcgccgc ctccatctcg ggccagatct
      421 cggcatggcg cttcttgtac tcgtcctcgt agccgggccg gacgcccagg acccaggcat
      481 attcgggcat ttttccttcc ttctcagacg ggctcgctgc ggcccgaccc aaacgagcgg
      541 tagatcgcgt ccagcgcctc gacggcacgg cagccgacaa cgcccggggc ctcgttggtg
      601 acggccgtgc ccaggcagat ctggacgagg cggttgacgg gctccgtgca ggtgtaggcg
      661 ccggcgccgg gctcgatcgg caccaccgtg tcccggccgt cgcggcggcg ggcctcgagg
      721 cgctcgcgct cgacgtcgaa gagcagcatg ccctccgtgc cgaagaggcg aagatcgagc
      781 tggaagccgc actgcttcgg cacggcgctg gagcccgaga cggcggcggt cgcaccgttc
      841 ttgaagttca gcgtggcggc caggtgataa tcgacgcccg ccttcgagcg cacgcaggcg
      901 gcgtaggcat ccctgggctc gaggtcggtg acgcggaaga ggaggcccag cgcatggctg
      961 agctggcccc agccatagcc gcccgcacgc ttgggatcgg cccaggtgga ggccggcggg
     1021 cggaagaggt ggtccctcgt ctcggccatg ggctcgccgg ccatgaggtc gtcgaggggc
     1081 gaagccatct gcagcacgac atgctcgatc cggccgatcg caccttctct gaccagctcg
     1141 tgcgccctgg tcgtgtaggg ctggaagttc cagccatagg gcaccaccag ctcgcggccg
     1201 accgcctgcg ccttcgccac cagggtgcgg gcatccgcag cgctcgtcgt catcggcttg
     1261 tcgaccagca catgcgcacc cgcctcgagc cccgccatgg cgtgctcgaa atgcaggacg
     1321 tgcggcgagg cgacgacgac ggcgtccggg cgttcctcgg cgagcatcct tgccgtgtcg
     1381 gtgtagcccc gggcgatgcc gaaatgctgc gtgacccgat ccagcgcttc cttgtccggc
     1441 cggttgacgg ccacgagatc ggcatcgggg tgcgccagga gcgccggcaa atagttgtgg
     1501 caggcccacc agccggcgcc gatcaccgca atcctggctt tcttcgtcat cgcgaggcgc
     1561 tcccgggctt gaaggagagg cggccggcgc tctggacgag gttgggcacc agcaccgaga
     1621 ggatcagcag caggccgatc accccggtct gcaggtggcc cgtgatgttg gcgagcgcca
     1681 tgccgtttcg aaggttgagg acgatcagga tggagaggag gacaccccag agcgtgccct
     1741 tgccgccgaa gatgctcacc ccgccgagca gcaccatggt gatgatgtcg agctcgaagc
     1801 cgagcgccac gtcgccgcgc acggcactca cccgggccgt gtagagcagg ccggcgaggg
     1861 cggcgacgaa gcccgaggcg acgaagagca gcgccttcac gcgcggcacc gccacgccgg
     1921 agtagcgcgc cacctcggcg ctgttgccga tgacatagac cttccggccg aagcccgagc
     1981 gatgcaggac gaccagcagg acgacgaaga gcacggcgaa gacgagaagc gccagcggca
     2041 ggggacccag gatcggctgc tggcccagcg cgttgaacca gtccggaaag ccggtgatgc
     2101 cgcggtcctc gaccaggatc cgcgccatgc cgcgaaagcc gatgagcatg gcgagcgtga
     2161 ccaccaggga cggcatgccg atgacggtga tccagaaggc gttgaaggcg ccgcccagaa
     2221 ggccgagggc gaggacgatc gggatggcga ccccggccgg cacgcccatc tggaagagcc
     2281 agccgaaggc gcaggcggcg aggcccatca tcgaggcgac cgagaggtcg atctcgccgt
     2341 tgacgatcac cagcgtcatc atcagcgcca cgatgatctt ctcgatcgag agctggaaga
     2401 gattgatctg gttgccgatg gtcaagaaca tcggcgccat ggccgagttg acggcgatgg
     2461 tgaccgccag gatcaccagc agcaggacct cccagcggac cagccggcgc atgagcccgc
     2521 cttccatcac ccggcctccc tgggcacggg cagggtcccg gccgagagct cggccatgtg
     2581 gcgcttgcgg cggagccggt cgagatagcg gttcatcagc gtgtcggcgg cgaccgcgat
     2641 caggatcagc atgccgagca gcgcgtcgcg ccagaactcg ctgaccagcg cccagcgcgt
     2701 caggctgttg tcgatgacgt cgaccagggt cgtgccgagc acgacgccga ccaccgtgcc
     2761 ggagccgccg aagatgttga ccccgccgac caccacggcg gccaccgact tgagctcgaa
     2821 gccgagcccg gcgaccaccg tgatgttgcc gaagcgggcg aggaagacga agccggcgag
     2881 cccggcaagg gcaccgctca ggaggaaggc cgtgaagacc acgcgctcgg cgttgatccc
     2941 ggcgatggcg gcggcgtccg ggttcgagcc caccgcatag agccggcgcg ctgccctgag
     3001 gcgggtgagg gccagatgca cgaccagcgc cacgcctagc gcgatggcga cggtcaccct
     3061 gatgtcgagg tcgccgatgg agaagagcgt cgcctgcggc agctcgacca cccacttggg
     3121 caggttcgcc gtggtgatcg aacgggcatc cgagtactcg accaggatcg tgcggtagat
     3181 ggcgagcgtg ccgagcgtca cgatgatcgc cggcacgcgg ccataggcga cgacgacccc
     3241 gttgactgcc ccgagcgcgg cgccgagggc catggccgcc gccacgacca cgagcgggtg
     3301 gaggtcgggc tgggcgacga ggagatcgcc caccagatag gcggtgaagc cgacgatcga
     3361 gccgaccgag aggtcgatgt tgcgggtgat gacgaccagc gcctggccga tggcgatgag
     3421 caggatgatc gccacgctgg tcgagatccg gttgaacatc cgcgcggtgt agtagttgtc
     3481 gatctgcgag ccgaagaaga ggacgacgag cgccagcacc agggcgagcg ccgtcacgcg
     3541 cagcgtctgc gggtggacgc tccgcagcca gccggtcatg cggcggcggc cggacgctcg
     3601 ccggtggcga gcgacatgat gccttcgggc gtggcgtcct cgatggcgaa gatgcccatc
     3661 tgccgcccct cgcgcatgac gaggacccgg tcggcgagcg ccatgacctc cggcaggtcg
     3721 gaggagatga cgatgatcgc cacaccccgg gcggcgaggt cgttgatgat cgcatggacc
     3781 tcggtcttgg cacccacgtc gatgccgcgc gtcggctcgt cgaagatcag gagccgcggc
     3841 tccgtctcca gccacttcgc cagcatcacc ttctgctggt tgccgcccga gagcttgccc
     3901 acctccagcg ccacgctgtg cgtgcggata ttgagccgct ccctgtgctt ctcggccatc
     3961 gccagctccg cccgtcggtc gatcaggccg agcttgccca ggagccgcct gaggctcggc
     4021 agcgagatgt tggcggagat cggcagcgcc atggcgaggc cgagcttgcg ccggtcctcc
     4081 gagacatagg cgatgccgtg ccccatggcg gccttcgggc tctcgatctg cgcggcctcg
     4141 ccgtcgagct cgatgctgcc ggaagtggcc ggctggatgc cgaagagggc gaggccgatg
     4201 tcggtccgcc tcgagccgac gaggccggaa aaacagagga tctcgccggc atggaggtcg
     4261 aagccgacgc cggtgaagac accctcgcgg gcgagccccc ggacacgcag cacccgttcg
     4321 ccgtgcggcc cggccgccgc cttcggcacg aaatggtcga cggcacggcc gaccatgtcg
     4381 cgcaccagcg catcgggcgt cacctcggcg atgggcttgg tcgagatgtg ccgcccgtcg
     4441 cgcagcaccg tcacccggtc agccagctcg aacacctcct ccagccgatg ggtgatgtag
     4501 agcacggcca caccctggcc cttgagcgtg cgggcgatcg ccatgagcct cgccacctcg
     4561 tgcgccgaga gcgacgccgt cggctcgtcc atgatgagca ccttgacgtc gagcgagatg
     4621 gcccgggcga tctcgaccgt ctgctgctcg gccagcgtca gcccgctcgc cgcccggcgc
     4681 acgtcgaggg tgacgccgag ccgctcgatc tcccggcgcg ccgcctcgtg cagccggcgc
     4741 cagtccatcc aaaggccgag gtcgcggtgg ctgatgaaga tgttctcggc cacgtcgaga
     4801 tcggggaaga ccatcggctc ctggtagatg gcggcgatgc cgagccgctg tgccgcctgg
     4861 gtgtcggcga tcgtgacggc cgtgccggcg aggcggatgg tgcccgcatc cggctggtgc
     4921 acgcccgtca tgatcttgat cagcgtcgac ttgcccgcac cgttctcgcc gaccagcgca
     4981 tggatctcgc cgggcaggag ctcgagcgag acatcggcca gcgccgccgt cagcgcgaag
     5041 ctcttggcga tcccgtccag cgccaggacc ggctcaggca aggagcgctg cccgagccgg
     5101 ccggaccgag cccagggtca ctgggcggcg gcttcgacgt tgtccgcgtt gtagaccgtg
     5161 aaggggccca tcagcacgcg attgcccgca tcgcgggtcg ggtccttctc gatcgagaag
     5221 gtgcccatcc ggccggcctc gaactcgtcg ccgacctcgc ccgcgatggc ccccgtggcg
     5281 atcaggtagc tcgtgtagta ggtgaggtag ccgagatcga cgaagctcca gagcgcgaac
     5341 tcgggcgcac aaccattcat ggtgtagctc accatctcgg ccggcacgcc gagccccgag
     5401 atcttgatct ggtcgcacag gccctcgtcc tgcatggcct tggccgccgc cacgatgccg
     5461 accgaggtcg gcgccatgat cagcttgagg ttggcgtgct tgtcgaccag cgccaaagcc
     5521 cggttgtagg agagctcgga ctggtcgtcg ccatagaccg tgtcgaccag ggtcatgttg
     5581 gcgtatttct cgccctccag cgcccgctgc atggcggcga tccaggcgtt ctggttggcc
     5641 gcgtccgggg tcgccgagag gatcgcgaac tcgccctcgc cgcccatgat gttgtgcgcc
     5701 atgtcggcca tgacgacgcc gatgtcgttg aagtcgacct gggcgacgaa gaccgtctcg
     5761 ccctcggccg aggggatcgg cgaatcccag gtcacgaccc tggtgccggc ttcctgcgcc
     5821 gcctgcgccg agggcacgat ctggtcgccc gagttgttcg agagcatcac cacgtcctgg
     5881 ccctgggtcg cggccgtggt catgatctcg atctggcccg ccacgctgtt ctcgggcgtc
     5941 gggcccacat agagcagctc gcccttgttc tcgagctcct cgtgcgcctc ctgggcgccg
     6001 cgatgcgcct ggtcgaaggg caggattccg aggaacttgg gcaggagcac catgtcgacg
     6061 ctctcgccct tggtcgccgt caccggttcg gcgagcgccg tcgggcccat gagcgacgaa
     6121 cccatgagca gcgctgtggc cgccaggcag cttgaacctg caaatttcat tcgagcctcc
     6181 cctgcttcgt gttctgattg ccatcggccg gagccgggtg gtcgagcggt aggagggcgc
     6241 tgtccccctg tcaagcaccg cccagattgg caagctggcc gctgccggct accgttctgc
     6301 gcgtgccgga aaacggggag gcaagaagaa gttgcaacag ctcggagcac tctcgctcac
     6361 ctcctggtcc ctgacgcact gcacgctcgg ggaggccgcc aacatcggcc gggccctcgg
     6421 catcgagggg ctcgatctcg gcctgctgca cggcccggcc ctggcgaagg gccgcctgct
     6481 cgccgacccc gaggcgctgg ccggcgagat ccgccggctc ggcatcattt gcccgaacct
     6541 ctactggctc ttcgggaatg gcttggtcga ccgcaacctg gccgaccccg aggcccgcgc
     6601 cgccaacgag gccgacttcc gccgggtgct cgccttcgcc accgccgcct cgatcccgac
     6661 gatcttcgtg ctgcccggcg tctgcgggcg gggcatcggc cgcgaggcgg ccttggagcg
     6721 ctccgccgcc tcgctccggc cgctcgccgc catggccggc gatgccgggg tcacgctcac
     6781 cgtcgagccg catgtcggcg gctttctcga atcgcccggc ctgacgctgg cgctcctcga
     6841 tgccgtgccg ggcctgaagc tgacgctgga ctacgcgcat ttcgcctgcc tcggcttcac
     6901 gcaaggcgag atcgacccgc tcgcccctca tgcggcgcac gtccatctcc gccaggcgcg
     6961 gccgggtgcg ctgcaatgca agctcgagca cggcacgctc aacttcccgg ccatgctggc
     7021 gacacttgcc ggccacggct accggggccg gctcgccatc gaatatgtcc accagggcta
     7081 catggacacg ctccacgacg acgtcctgag cgagaccatc aagatgcgcg acctcgtgcg
     7141 gcttcagacg gcgtcgccgg cagcacggta gacgctccct tcgcttggct ggcgacagtg
     7201 cccggcatcg gccgcaagcc tctgcgccgc ccatcatcac gaacgcttat ggccatcgcg
     7261 ccccgggtgt cccggggccg tccatgcagc gccgattcgg cccctcggcg ctcgggttgt
     7321 cgcggattct gcaacatgcc ggctcgggtt gtcgcgtctg gcgcaacagt gcctgcccga
     7381 atgtcgcgga ttccgcgaca taacgattcg tgatggcgcg tccggcgcga cattcgcccg
     7441 caaatgtcgc ggattccgcg acacgccacc gcgaacgaac ccgcggattc gacgagcgaa
     7501 cccggggatt cgacgagcga acccggggaa tcgacgaacg aacccgaccg tcgaccaacg
     7561 aacccgagaa ccatcaagac attgtaacga aaggtgtttt accgctttcc gtcggcccgg
     7621 cgccgggccg tgtaccgagg tttcgtgaaa ttgccgaacg aacccgcatc gggcttgcat
     7681 ttcgtccgga tatgagctaa tattcccata ggtggaacga gcccgatctg cctgctcccg
     7741 cctccggtgg cggcgctgag cgaagcgcag cttgcggcgg caccggccaa cgcctacctc
     7801 tccgccatgc cagaccccgc ccccgacctc gcccgcccgc gcatcggcat caccctggac
     7861 agcgaggagc ccggcggcta ctcgaagttc ccctggtacg cgttgcgtca gaactatggc
     7921 agcgccgtgc tcgccgccgg cggcctgccg ttcgccctgc cgcatgtgcc gcgggcgcag
     7981 gtcgacgcct atctcgacca gctcgacggc ctgatcgtga ccggcggcgc cttcgacatc
     8041 gatcccgccc tctatggtgc cgtcgagcgg cacccgaccg tcgatctgaa ggcggcgcgc
     8101 accgccttcg aatgggcgat caccgagcgc gccctcgccc gcgacctgcc ggtgctcggc
     8161 atctgcggcg gccagcaact gctcaatgtc gtgctcggcg gcacgctgat ccagcacatc
     8221 ccggacgtcg ttcccgacgc cctggcgcac gagcagccca acccgcgcaa cgaggcgagc
     8281 cacgtggtcg acatcgtgcc gggctcgctc ctgcaccgga tctgccgcca tgacgtcctg
     8341 gcggtcaaca gcgcccacca ccaggcggcc gaccgcacgg gccgaggcgt cgtggtctcg
     8401 gccaccgccc cggacggcgt ggtcgaggcg atcgagcacc cgggctaccg tttctgcctc
     8461 ggcgtccaat ggcatcccga gttcctgatc agcgagggcg acctgccgat cctgcaggcg
     8521 ctggtccggg cggccgcgcg atgagcgagg ctccagacgg cacccgaccg gccggcgccc
     8581 gactaccggg tgaaagaatc gccaagcgga tcgcccgtgc cggctactgc tcgaggcgcg
     8641 acgccgagaa gctgatccag gagggtcggg tcagcatcga tggccgcacg gtggagagcc
     8701 cggcgctcaa cgtcacggcc gggcaggaga tccgcatcga tggcgagcgg ctgaaggcgg
     8761 cggagcctgc ccgcctgttc cgctaccaca agcccgctgg cgtggtgacc acggcgcacg
     8821 acccggaggg ccggccgacc gtcaccgaca agctgcgcgt acgcacggcc gacggcagcc
     8881 tgccgcggct gatgccggtc ggccggctcg acttcagctc cgaggggctc ctgctgctca
     8941 ccaacgatgg cgggctcaag cgcctgctcg agctgccggc gaccggctgg ctgcgccgct
     9001 atcgcgttcg ggtctacggc aacgtcgatc ggaacgcgct cgaatcgctg gccgccggca
     9061 tcgagatcga cggcatccgc tacggcccga tcaaggccga ggaggagagc cgcaccggtg
     9121 ccaatgcctg gctcaaggtg ggcctgcgcg agggcaagaa ccgcgaggtg cgtcgcgtgc
     9181 tcggccatct cggcctcgag gtcagtcgcc tcatccgcac cgcctatggt cccttccagc
     9241 tcggcaagct ggggcgcggc gacgtcgagg aggtgacgcc caaggtgctc aaggagcagc
     9301 tcggcggcct cggcgccggc ctcgcgctgc cggaatccaa gagccatagc cacgagcgtc
     9361 gcctgacgcg gccggtcgcc ggcccggctt gagcccgccc cggatcatcg ccgggcggca
     9421 tcgtggccgc cgcctcgagg tgccggctgg tgatatcgtc cggccgagcg ccgagcgact
     9481 gcgcgaggcg gtcttcagca tgctcgagca cgggcagccg cggctgcgcg attgccggtt
     9541 cctcgacctc ttcgcgggct cgggcgccat cggcctcgaa gccctgtcgc gcggcgccga
     9601 cgaggcgtgc ctggtcgagg cggaccgccg ggcggccggc gccgctaggc gcaatatcga
     9661 gaagctcggc gagaccgggc gggcgcggct cgtggtcgcc gatgccacgc ggctgccacc
     9721 ggccggcgag cccttcgcca tcgcctatct ggatccgccc tacggcagcg gcctggtgcc
     9781 cgcctgcctt gcctgcctcg ccgccggtgg ctggctcgtg gcgggggctc tcgtcgtggt
     9841 ggagatcgcc gcgcgcgaag cgctggcgcc gccccagggc ttcgactcgc tcgacgagcg
     9901 ccgctacggc gccgggcgga tcgtccggct gcgctttgcc cctggcaact gaccgttccc
     9961 gccgcggcgg ctaaccaaac ctaaaggaaa gcctgccatt ctcgggccag cccgcaattc
    10021 caacggcagc ccatgcgcgt cctcacgctt cttctcttca tgctcgcgat cttcctcatc
    10081 aacgcgcatt tcatccgcgt cggcgaggag gagaaccgca tctggccgcc catggtgctc
    10141 gcgctcctga gcctgattct gctcctgcgc acgcccaatc tcctgcgcgt caactggaag
    10201 ctgctggtca gcccggtcgt cctgctcttc gcgctcaatc tcgtctggct cggcatcgtc
    10261 cagttctggt cggtggcccc ggccggcggg cgcagccaca tcatcctgct ctggctcacc
    10321 ctggtggccg ccctcgccct ttccgaggag cgtccctatc gcagcgtgct ggccttcgtc
    10381 ggctgcctct gcatcgtcta cgcctggagc tgggccggcg ctgccgccgg acaggactgg
    10441 gtggtgctcg accgctcgca atggcggctc aagggcgtca tgatgcaccc gcagacgctg
    10501 gctctcgtcg cgatcaccgg agtgatcgtg gggctcgtgt ggcagctcaa ccggcagacc
    10561 gccggtgcgg tgccgcatcg cggccggatg acggccttct tcctggtctc gctcgccacc
    10621 ctgctggcga cgcagggccg ctcgctcacc gccttcttcg tggtgacgct cttcgtcgtc
    10681 tacttcttcc acatcaaggg gccgaagcgg atctgggtcc tgctcgccgg gatcgccatt
    10741 ggcatcgccc tctacgtcgc cgtcgacatc atcctgccga tgatcagccg gggtcaggag
    10801 gaaaccctct ccggccgcac gatcatctgg gagatgaccc tgcgggagat cgccaaggca
    10861 ccgttcctcg gcttcggctt cggcacctac caggactatt tctccagctt ctacaacaac
    10921 tggtcgcccg gtcacgcgca caatctctgg ctgcaggtca ccttcgaatc ggggctggtc
    10981 ggaacagttc tctacacctt cttcctgctc gccctgatgc agcgcggcta tcgcttccag
    11041 cgcgacaccg gcctcgtctc ctacacgctc tgcctcacga tcttctgcct cctgggtggc
    11101 atgaccagcg tctttctcgg cgagaagctg tcgacgctct atggcctcct gctgctcctg
    11161 gcgctgcagg aggagcggct gcgactggag gcgctggcgc gtcagcgggt cgggaccctg
    11221 ccgctccatc gaccgtccgc ctcgccttcg gggaccgagg ccagaccgcc aggcgccgag
    11281 ccgcagcctg cctgagcggc tcgcctggcc ggcagccgcg ccgggcagcc atccgatgta
    11341 ttgcgatagc atgatacgaa aagcggcaag ctcgtcgagc gctgacgccc atgacgttct
    11401 atcagccctt tggtgtcgtt gcgggtcgag catggtcgga aacggaaacg aaacgaacct
    11461 tgccgcggcc ggccggtgga cccgggaccg ggtccggctg gccgggctct tgtcggccgg
    11521 cggggcgtcg ttgtgggtcc cggcaggacg cgtctcgacg ggggcggatc cgggcgccac
    11581 gatcgggcga tggcgggcca ccgccgaccg cgccggagtg cgttcgtgac cggcctcgat
    11641 gaccaactcg ccggagcggc cgggagcctc gccggcatgg cctatctcgg cctcgccctg
    11701 ctgctcggcc gccgtccgcg ggcgcccgcc tcggcgccga tcctgcccct ggtcattgcc
    11761 gttctggctg gtgccttcgc gggctttctt gcaggcgtcg tcggcccggt ggtcgcccgc
    11821 ccggcaggct ttgtcgaaac gctttgctgg atcgcttttc tcgccttcct cggccatgcg
    11881 ctggccgatt cggccggccg ctggctcgct gccgcgatcc ttccgctggc cgccgcgatg
    11941 gcgtcgatgg cgcccttcga tccctggctg atggccactg gcggtgctgc cgtgatcggc
    12001 ctctttctgg ttctggcgca gggcctcgcc cgtgcaccgg gcgaggccgg caggcggctc
    12061 ctgctcctcg ggctcggcgt cggcttcacc ttcgatctgg tgatcgccgg cctggctctc
    12121 gccgatgcgc ccttgctcgg ggcggccgag gcagcgcggc cgctgcttcg cgccgcgctc
    12181 gtgccactga tcgccatggc caccagcccg gtgctggccc ggcccggccg catcgtgccc
    12241 tcgaccacgc ttcgccggtt ggccctgctc gtcctcgcga ccacgctcgc ccttctcgcg
    12301 gtcgccgccg gggccggctg gctcggcacc aggctgggtg ccgccctggc gctcgccctc
    12361 ggcctgcccg ccggcctctt cgtgctcgct gccggcctgg cgccaggcgc cgggtcgcgg
    12421 ctggacaacc tgctgcgccg ccatttgccc gtgcatcgcc acgactaccg gcaggtctgg
    12481 cccgcgttcg tcgacgtgct caccggcgcc gatccacggc cgagcgacga tctgccggaa
    12541 cgcgtcattc gcgctgtcgc cggcacggtc ggggccgccg gtggcggcat ctggctggcc
    12601 gagccgggcg accgcttccg ccgcctcgcc agcctcgcct tgccgtcccc cgccgagacc
    12661 gccggccggg caccggccct ggcggccgcg ctcctcgccg ccgaggggcc cctgtcgatg
    12721 gcggacccgc tgcccggtgg cggccagtgg ccggccttcc tgccacgtcc cgccgccgcc
    12781 tggctggcct tgccgctgat ccacaaggag gcgcttttcg gactcctggt gctcgccgga
    12841 ccgtccgggc gacctcacct cgacgacgag ggccgcgagc tgctgaggct cgtggcacgc
    12901 caggcggcct cctatctcgc cgaggacgag accgccaggc agctcgccga cgcccgccag
    12961 ttcgagagct tcactcgccg cttcgcctat gtcatgcacg acctgaagaa cgtcggcagc
    13021 gagctggcgc tgaccctcgc caatgcccgt cgtcatcgcg acaacccggc cttctacgac
    13081 gacctgctgg agacgctgga agcctcggtc gatcgcctcg gccggctgat cgcccagctc
    13141 cgcaacgagc gccagccgcg cctcgagccg gtggccctgg cgccgctgct gcgccgcctc
    13201 gtccagagcc gtgccgatcg tcgcctcgct ttggcggaac cattgccgtc gctcgctgcc
    13261 cgggtcgagg ccgatgcgct ggagatggcg ctcgatcatc tgatcgacaa cgcgctcgcc
    13321 gcactcgatg ccggaggacg cgtcacgctc ggcctcgtgc ggcgcgaccg catggcgacc
    13381 gtgctggtcg aggacgacgg cccgggcttg ccgcccggcc tcgtcgacgc cgccggccgt
    13441 ggccgtccgt tcacgagctg ccggccgggc ggcctcggtc tcggcatcga tcaggcccgc
    13501 aacacggtcg agcgggtcgg cggccgtttc gagctggtga gccggccggg tcgcggcacc
    13561 gcggtggcct tgcatctgcc ggtcctggag gaagctgccg gggaggaagt cgccgcatga
    13621 ccgagcggcg cctgctcgtc gtcgaggacg atgtcgctct gcagaagcag ctgcgctggg
    13681 cgctcgacgc cttcgctgtg ctgccggcca ccggtcgcga gcaggcgctc gccatgctgc
    13741 gcgccgagga gccccccgtg gtgctgctcg acctcggcct gccgccggac ccgtgcggcg
    13801 tcagcgaggg cttcgccacg ctccgccagg ttctcgacct cgtgccgtcg accaagatcg
    13861 tcgtgatgac cggccaggag gggcggcagc acgcgcgcga tgccattgcc gccggtgcgc
    13921 acgacttcca ccagaagccg gtcgaccccg accagctcgg ccttctgctc gctcgcgcct
    13981 tctatgtcgg cgagctcgag gcggagaacg agcgcctgca ggcgcgcgcc cggcagagcc
    14041 ggatgcccgg catcgtcggc gacagcccgg cgatgctcaa gctctaccgg gcgctcgagc
    14101 ggtcggggcc gaccgatgtc agcgtcgccc tgctcggcga gagcggcacc ggcaaggagc
    14161 tcgtggcaca cgcgctgcac tccctcagcc cgcgccgcga ccgccgcttc gtcgcgatca
    14221 attgcgccgc catcccgccg accctgctcg aggccgagct cttcggctac gagcgcggcg
    14281 ccttcaccgg cgcctttcgc cagacccccg gcaagatcga gctcgccgac cgcggcacgc
    14341 tcttcctcga cgagatcggc gatctgccgc tcgacctcca ggccaagctc ctgcgtttcc
    14401 tccaggagcg tgtcgtcgag cggctcggcg gccgccagga gatcgccatc gacgtccggg
    14461 tcgtctcggc gacgcatcgc aagctcgagg atctcgtcgc ccggggcggc ttccgcgagg
    14521 atctctatta ccgcctggcc gagctcagcc tcgagatccc gcctttgcgc gaccgcgccg
    14581 gcgacgctct cctgctcgcc cggcacttcc tttcccgctt cggcccggag ctcggcaagc
    14641 agcgcatcgg cgtctcggcc gaggcactgg ccgcaatcga cgcctatgcc tggcccggca
    14701 atgtgcggga gctgcagaac cggctgaagc gggcgatcat catgtgcgag ggcaggcgca
    14761 tcggccgggc ggatctcgat ctcgccgccg aggacgaggc ggatgcggtc ctcaatttgc
    14821 gtcaggagcg cgagcgggcc gagctcggcg ccttgcggcg ggcgctggcc cgtgcggaag
    14881 gcaatatctc gcgcgccgcc cggctgctcg gtgtcagccg cccgacactc tatgatctcc
    14941 tgcgccagca cggcctgcgt gactgagaga gggcgtgtca gataaggtgt attgtgtaaa
    15001 tgaaatcgac ggtattttga tattgctgcc agttgcccgc tgtttttctt gacgccttgc
    15061 gtaccgtcat gcaccacatg atcgtgttgt tgaagccagc gtcgcgcgcc ttgcagccac
    15121 cggacatgac gcgcacggca agcgatccgg tcgtggtcgc gaatgcaatg gtggtggcgc
    15181 tgccgacatc ggcttcgagg cgaggggacc tcatggtccc gaacttcaag ggcccgcccg
    15241 gataatcggg gcgggccctt tttttggtcg cggtcgcggt cgccgcgtcc ccggcatcga
    15301 cccgatgcca acctggcacc ggcacgctcg ccgcttgcgc cggcggctcg ccgtggcaca
    15361 ttgcgtggcg gcggccccgc ccgaacccgc cccggccgcc tcgagcaggc gaggtcggtg
    15421 acggatgcag gatcgaagcg cctcgcggcc cgcggccgcg ccgaccggtg ggccgccgcc
    15481 tttggtggtg tcgccaggcc cccgcccggc tgccggcgga cggccggagc gctggtggcg
    15541 caggctcggc cggctcggca cgaccggcga gatcggggtg cggttcttca cgctcgcggt
    15601 tcttcgctgg atggcgctgg tcggccagac cttcaccata ctcgtggtcc atttctcgct
    15661 cggcatcgag cttcccatgc tctggctcgt gccggcgttg ctgctcaccc tcgcggtcaa
    15721 tgtcggggtc caggtcaggg gcacgcccac tgcccggctc ggtgccaggc agaccttctt
    15781 cctgaccctt ttcgacgtcc tgcagctcac ctttctgctc tttgtcaccg gcggcgtgca
    15841 gaatcccttc acggtgcttc tcgtgctgcc ggtcggtctc gctgccgcga ctctggaccg
    15901 ggcaccgacc gcgatcctga ccgccgtcgc cctcgccgcc atcggcttca tggccgccat
    15961 tccgatggag ctgccctggc tcgacggcgg gctgcgcctg ccggcgctct atctcttcgc
    16021 cgcctggctg gcgctgtcgc tgacggtggt gctggtcgcc gtctatgtct gccagctcgc
    16081 ctcggaggcc cggcgccatg cccttgctct gtcggccctg cagctcgccc tcgcccgcga
    16141 gcaggagctc tcggccctgg gcggccaggc ggcggccgcc gcccacctcc tcggcacgcc
    16201 gctcgccacc atcaatgtca tcgccaagga gctggtccgc gagctgccgg ccacgagccc
    16261 gctcctcgag gacgcgagcg agctgctcct gcaggcgcgg cgctgccgcg agaccctcgc
    16321 gagcctgggc agtggccagc gcgacgcggc gcatgccgcc ttcaccaggg caccgctctc
    16381 cggcctgctc gccaccatcg ccgaaacctg tcgcatcgag ccggtgacca tcgatattgt
    16441 cgtcgacgtc gagccggggc tcgaggagcc gcgggtcgtg ctcgccccgg agctgcgcca
    16501 ctcgatcgcc aatctcgtcg acaacgccgt ccgcttcgcc cggacacgag tcgacatcac
    16561 ggttgccgtg gcgcatgccg gcattgccgt cgagatcgcc gacgacggcc cgggcttcac
    16621 gcctgaggtg ctcgactggc tcggcgagcc ctatctctcc acccgccggg ccgagggcgg
    16681 actcggcctc ggcgtgttca ttgccaagac gctgcttgcc cgaacaggtg cttcgatgca
    16741 ttttgccaac cgagacaagt tgtctcaccg ggaagaaggc gccgtggtcc gcatcgtttg
    16801 gccatccggc gccctggaaa tgctcggacg ggagtccgcc gatgacggat gaaggcctcg
    16861 atgtggccgt cgaagacaaa cctttgcccc ggttcgtcgg gtcggatgcg ccggtgctca
    16921 tgctggtcga cgacgacgca ccgctgcggc gcagcctgca gcgcgccatg gagcggcgcg
    16981 gctttcaggt ctatgccggc gagagcctga aggaagggct gaacatcgct cattcggtga
    17041 agccggctta cgcggtgatc gacctgagat tggaggacgg ctcgggcatc gacctggtcg
    17101 cccggatccg cgagctgcat ccggcgatcc gcatcgtcgt gctcaccggc tacggcaata
    17161 tcgccacggc ggttgccgcc atcaaggcgg gcgcggtcga ctacctcgcc aagccggccg
    17221 acgccgacga cgtcatcgcg gcgctgctcg ccaccggcag cggcctgccg ccgccaccgg
    17281 cgaacccgat gtcggccgac cgggtgcgct gggagcatat ccagcgggtc ttcgagcagt
    17341 gcaaccggaa cgtctcggag acggcgcggc ggctcaacat gcatcgccgc acgctgcagc
    17401 gcatcctcaa caagcgggca cccaaggaat aagagatcac gccgcatccc tgtcgcctgt
    17461 cagaagcgcc gggcggggtt gcgagaaggg gcggggacgc ggccagtcaa atgcagcacg
    17521 aaggggcacc gaatgatcgc cggagccgat agcaagagcg ccgcaccggc ggaaccgacc
    17581 gcaaagaagc tcctgctggt cgatgacgac gcgccgctac ggcgcaatct cgtccgttcg
    17641 ctcgagcgct tcggcttcgt cgtcgccacc gcggccagcg tgcgcgaggc ctatgaggtg
    17701 gccagcgagt tcctgcccga gctcgcggtg gtcgacctca acctcgagga cggtcacggc
    17761 atggagctgg tcgagagcct gcaggagctg cgcccgggcc tcagggtcgt ggtgatgacc
    17821 ggctatgaca gcatcgcgtc cagcatcgtc gccaccaagg ccggtgcggt cggctacctg
    17881 gcaaaaccgg tgaagcccga ggaactcgtc gccgtgctcg acggctcggc cgagcctgcc
    17941 gtcgacgaca gcccgatgtc ggccgaccgg gtgcgttggg agcacatcca gcgggtcttc
    18001 gagcagtgca accgcaacgt ctcggagacc gcgcggcgcc tcaacatgca ccgccgcacc
    18061 ctgcagcgga tcctgagcaa gcgggcgccc aggccctgag tctcgcgcca tgagtcgacc
    18121 gatcgaggat tacgcgctga tcggcgacac gcgcaccgca gccctggtcg gcaaggacgg
    18181 gtcgatcgac tggctctgcg tcccgcgctt cgattcgccc gcctgcttcg ccgccctgct
    18241 cggcgacgaa ggcaacggtc actggcggat tgcgccgatc gacgccacgg ccaccgtgcg
    18301 ccgccactat cgcgacgcga ccctggtcct cgaaaccgag ttcacgaccg atgcgggcgc
    18361 ggtcgcggtc gtcgatttca tgccgcagcc gcgcaatgac gggcaggtcg atgtggtgcg
    18421 cctcgttcag ggccgccgag gtgctgtcga catgtgcacc gagctcgtgc tgcgcttcga
    18481 ctacggatcg gtcgtcccct gggtcgagcg tcgcggctac ggcctgcgcg cggtagccgg
    18541 gccggacgcc gtccgtttcg aatcgcccgt tgcgctggag aacgagaact tccgctccat
    18601 cgggcggttc accgtgcgcg aaggcgagac ctggcccttc cggctcacct ggttcccgtc
    18661 gcatcgggac gggccggtgg tcggcgagtg gcaggcggag ctcgagcgct gcgaggcctg
    18721 gtggcacgac tgggccgagc gctgcaccta cgagggcccc tatcgcgacg ccgtgctgcg
    18781 ctccctgatc gtgctcaagg cgctgaccta cggcccgacc ggcggcatcg tggcggcagt
    18841 gaccacgtcg ctgcccgagc gggtcggcgg tgcccgcaac tgggactacc gctactgctg
    18901 gattcgcgat tccacgctga cgctcgatgc gctggcctcc tcgggctttc tcgccgaggc
    18961 ccgcgcctgg cgcgactggc tgctccgggc ggccgccggc aagccgtcgc aattgcagat
    19021 catgtacagc gtgaccggcg agcggcggct tcaggaagcc gagctgccgt ggctccccgg
    19081 ctacgaaggg agccggccgg tgcgcatcgg caacgccgcg catggccagg tgcagctcga
    19141 cgtctacggc gagatcatgg actccttcca catcgacagg gtgcatggca tgccggcgag
    19201 cgacgctgcc tggcagctcc agcgcgtgct gctcgaggcg ctcgaaaccg cctggcggca
    19261 gccggacgaa ggcatctggg aggtgcgcgg gccgccccgc cacttcaccc attccaaggt
    19321 catggcctgg gtcgccttcg a
//
LOCUS       DAOTFI010000077        19124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3448_length_19124_cov_7.024910, whole genome shotgun sequence.
ACCESSION   DAOTFI010000077 DAOTFI010000000
VERSION     DAOTFI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19124
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3448_length_19124_cov_7.024910"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="P5558_09725"
     CDS             <1..1302
                     /locus_tag="P5558_09725"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRY24630.1"
                     /translation="AEIETDKATMEVEAVDEGTLGKILIDEGSEGVAVNTPIALLLGD
                     GEDKSALDGYAAGGNDAAEAAPQAEAEEQPAEQAKTEPQPRRQALPTEPVQPEEPEVP
                     AGTEMVKTTVREALRDAMAEEMRRNGDVFLMGEEVAEYQGAYKISQGLLEEFGPRRVI
                     DTPITEYGFTGLGVGAAMAGLRPIVEFMTWNFAMQAIDHIINSAAKTLYMAGGQMGSP
                     IVFRGPNGAAARVAAQHSQEYSSWYSHCPGLKVVCPYSAADAKGLLKAAIRDPNPVVF
                     LENEVLYGQTFDVPKLDDFVLPIGKAKIVRPGSDVTIVSFSLMMTRALEAAEALAEEG
                     ISAEVIDLRTIRPLDRATVVRSVKKTNRLVSLEEGWPQSGIGSELAAVMMEDAFDYLD
                     APVIRVTGKDVPMPYAANLERMALPQTKDVVEAARAVCYKS"
     gene            1325..2674
                     /locus_tag="P5558_09730"
     CDS             1325..2674
                     /locus_tag="P5558_09730"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HRY24631.1"
                     /translation="MPIKILMPALSPTMTEGNLASWLKKEGDSVAPGDVIAEIETDKA
                     TMEVEAVDEGRLGKIVVPAGTEGVAVNAVIGLLLEEGEEESALEGAADGGADGGNGEA
                     KVAPQGAPPADPARAESPRTEGPREGAEKAAAGPAPAGGSGNGSGERIFASPLARRMA
                     EKAGLDLAALSGSGPRGRIVKADIEAALEKGGADAAPAKEKAAEKPAAAPAPAPAAAG
                     APDAPHTEIALSNMRKVIARRLTESKQQIPHFYLTVDCELDALLKLRKDLNERPGADY
                     KLSVNDLLIKASAIAIRQKPTVNSIWGGDKLYQLSDIDVSVAVAIEGGLITPIIRKAD
                     QKGLATISAEMKDLATRAKAGKLKPEEFQGGGFSLSNLGMFGIKEFSAVINPPQASIL
                     AVGAGEQRPVVKNGALAIATVMSVTLSVDHRVVDGALGAEWLQAFKKLVEDPISLML"
     gene            complement(2671..3123)
                     /locus_tag="P5558_09735"
     CDS             complement(2671..3123)
                     /locus_tag="P5558_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRY24632.1"
                     /translation="MLASFDSNVLIYAHLEPESIKGLAAVELLDRAGLAGAVIPAQVL
                     GEFLWVVRRRRPDCYIDALELVETIPSTMLVPSSTGDRMAKAARLTERHKIQFWDALI
                     CICAAVAGATHLLSEDMQNGAVLEGLRVINPFDPTNRPVLERLLPPLA"
     gene            complement(3124..3384)
                     /locus_tag="P5558_09740"
     CDS             complement(3124..3384)
                     /locus_tag="P5558_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRY24633.1"
                     /translation="MRTVSIRDANQQFSRLIKEVEAGETVVITRQGKPVAQLSPRPLN
                     RLDDPEVRTAYERWSALLHARKGDGYRVGNITEEDKYGPIED"
     gene            3503..4903
                     /gene="lpdA"
                     /locus_tag="P5558_09745"
     CDS             3503..4903
                     /gene="lpdA"
                     /locus_tag="P5558_09745"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRY24634.1"
                     /translation="MADTSFDIVIVGGGPGGYVAAIRAAQLGLKTAVVEREHLGGICL
                     NWGCIPTKALLRSAEIYDYFGHAESFGLSVEKIGFDLAKVVKRSRGVASQLSKGVAHL
                     LKKNKVTVFDGAGSLAGKGKLAVKATKGDDVTLDAKHIVLATGARARQLPGLETDGKL
                     VWSYKEAMVPDVMPKSLLVVGSGAIGIEFASFFRSMGAEVTVVEVLDRVLPVEDADIS
                     AFAKKQFEKRGIKCLTKAQVKGLKKGKDNVTATIEAGGKSQELTVDRVIMAVGIVGNV
                     ENLGLDRTAVKVDRAHIVIDEWCRTGEPGVYAIGDVAGPPWLAHKASHEGVMCVERIA
                     GVKGLHPMDVTKIPGCTYCNPQVASVGLTEAAAKEKGHKVKVGRFPFIGNGKAIALGE
                     PEGMVKTVFDAETGELLGAHMIGAEVTELIQGYVIARQVEATELDLMHTIFPHPTLSE
                     MMHESVLDAYGRAIHF"
     gene            4953..5837
                     /gene="lipA"
                     /locus_tag="P5558_09750"
                     /pseudo
     CDS             4953..5837
                     /gene="lipA"
                     /locus_tag="P5558_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            5845..6327
                     /locus_tag="P5558_09755"
     CDS             5845..6327
                     /locus_tag="P5558_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRY24635.1"
                     /translation="MPTHAEQRHLPYRPEQLYELVAGVERYPEFLPWCKASRITRREG
                     DVFWADLVIAFKVFRERFTSKVTLIPKTGVDVEYINGPFRYLNNHWRFLSVPEGGCIV
                     DFYVDFEFRSKMLQSLIGLLFDQAVHRMVAAFETRADQLYGKGKAADDGLETAVHSPS
                     "
     gene            complement(6333..6806)
                     /locus_tag="P5558_09760"
     CDS             complement(6333..6806)
                     /locus_tag="P5558_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRY24636.1"
                     /translation="MKTAARLLARCRERGLTLATAESCTGGLIAACLTEIAGSSDVVD
                     RGFVTYSNAAKTEMLGVAAATLEHHGAVSAETAAEMVRGALARSPADLAIAVTGIAGP
                     GGATATKPVGLVFIGAQRRGGAAEVERHVFPGDRRAVRTAALERALALLERAAIG"
     gene            complement(6830..7984)
                     /locus_tag="P5558_09765"
     CDS             complement(6830..7984)
                     /locus_tag="P5558_09765"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HRY24637.1"
                     /translation="MECVALVMAAGSGLRLGRERPKQYQALAGLPMLRRTIDALRACP
                     ELRELQVVIGAGQDDLYDAATRGLELPPPVIGGATRQASVRRGLEALARRAPTHVLVH
                     DAARPFASPALVGRVRQGLASARAVLPVLPVVDTLKRVDANGDVVAEMPRDGLGRAQT
                     PQGFDFATLLLAHRRHADRDCTDDAGVVALDGVPVLAVAGEEENFKVTTEADMARAEA
                     LLARQPRRWISGSGFDVHAFAPGRPLVLCGITLPHDRGLAGHSDADVALHAVTDALLG
                     GIAEGDIGSHFPPSDPRWRDVASAHFLHHATGLVRARGGRIEHVDLTIIGERPKIGPH
                     RDTLRASLAGLLALDPGRVSVKATTTERLGFCGREEGLAAQATVSLSFPL"
     gene            8155..9285
                     /locus_tag="P5558_09770"
     CDS             8155..9285
                     /locus_tag="P5558_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY24638.1"
                     /translation="MTAMQSGFSPPAPAHRPAVDPLVVLENLPSPVLVLAGRVLVFAN
                     PAAEQLFSLGRSVLLGRSLDDLLAPHSTLAALIGQLEEGAGSISEYGIELALQRGVVV
                     AVDAHITPVPEAQDHILLVLHPCSMAQRLDRIPAGRSNGRSIAGLAETLAHEVKNPLS
                     GIRGAAQLLAPSLGEEDRSLVQLICDETDRICALVDRMDAFSEPGRIDRRPVNIHLVL
                     EHVRRLAQNGFARHLRIVEHYDPSLPDVEGDRDQLIQVFLNLVKNAAEAAPAEGGQIT
                     LSTLYQHGFRMSVGAAGERHELPITVEVRDTGPGVAPDMIDHLFQPFVTSKAKGSGLG
                     LSLVAKIVGEHGGLVSYTRAQPGSIFKVRLPTARTIQGVGPT"
     gene            9282..10718
                     /gene="ntrC"
                     /locus_tag="P5558_09775"
     CDS             9282..10718
                     /gene="ntrC"
                     /locus_tag="P5558_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HRY24639.1"
                     /translation="MSGETVLIAEDDAAIRTVVSRALSRQGFHVQATSVAATLWRWIA
                     AGEGDVVITDVGLPDENSLDLLPRIKQLRPDLPIIVMSARNTLLTAVRATERGAYEYL
                     PKPFDLSNLLATVQRSLDRRASRPQRAEEPGLHEEQLPIIGRAPAMQEIYRVIARLAA
                     TDLTVLIAGESGTGKELVARALHDFGKRRDGPFVPVNMAAIPRELIESELFGHEKGAF
                     TGAGARRAGRFEQAEGGTLFLDEIGDMPIEAQTRLLRVLQQGEFIPVGGSNPIRANVR
                     IVAASHRDFRQMVSQGLFREDLYYRLNVVPLRLPALRERADDVPALVQHFLGKAHEEG
                     LPVKTIDPDAMRRLKEHNWPGNVRELENLVRRLAVLCSEEIIGESVIQSELEDSRRPV
                     EGEPVDETLSGAVARHVDRYFAAHDDELPPPGLYARILEELERPLITRTLQATRGNQL
                     RAADILGLNRNTLRKKIRELDIHLVRSP"
     gene            10838..13066
                     /locus_tag="P5558_09780"
     CDS             10838..13066
                     /locus_tag="P5558_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRY24640.1"
                     /translation="MLHRLRRFDFERKLALLLSILAVVSGIATYVVISTAPPYGRSVQ
                     TVVLLLNVDLILLLLLGVIIGRRLVMLVLQHRQGRAGSKLHGRLVALFAVVAVAPAII
                     VSVFSVLFLSSGLETWFGDRIRVALETSMNVAEAYLEEHKENIRADALAMAADLNREA
                     PYLLGDPVRLQQFVAGQAAVRSLTEAIVFDRLGNVLARTGLSFGLEMEYLPPGALTRA
                     ESGEVVTVTSSADDRVRALVQLFGFGDAYLFVGRFVDAEVLGFMQRTQSVVREYQQLS
                     TERRGIQITSAILFGLVALLLLFAAVWTGMAVADRIVAPIGQLIGAADRVRTGDLLAR
                     VPEGASDDEIAVLSRAFNRMATQLHSQRRELIDANHQLDARRRFTEAVLSGVTAGVIG
                     LDDQRRIVLPNRAASHFLESAAGGEIAGVDIQHVLPETLPLFERAELAAGQTVQQQVT
                     VRRGLAEYTLLVRLAAQREGDAIVGYVLTFDDITVLLSAQRQAAWAEVARRIAHEIKN
                     PLTPIQLSAERMGRKFASQIDEADRSSFHRAVDTIIRQVDVIGRLIREFSAFARMPDA
                     RMRPEPLAALVQHAADLQQSARPELRLTVDLGDSAGLYLDCDGEKISQALTNVLQNAV
                     QAVEESAAGADQEGGGSPAPVPVVEVTVLRGDHELAILVADNGSGFPAGERQRFLEPY
                     VTTRAKGTGLGLAIVHKIMEEHAGRVELDNSELGGALVRLVFPAERIVEGRPRPIGNR
                     AS"
     gene            13130..14509
                     /locus_tag="P5558_09785"
     CDS             13130..14509
                     /locus_tag="P5558_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY24641.1"
                     /translation="MSKEVLIVDDEAAIREVVAAVLEDEGYVPRQAATSDEALREVAR
                     KPPALLLLDIWLEGSRMDGVQILDHVRSSNPSLPVIMFSGHGTIETAVAAIKKGALDF
                     IEKPFKSDRLLVAVERAMELSRLRRENEELRSRAQKVELIGGSPVMGKLRATIQRVGP
                     TGSRILISGPPGSGKEVVARMIHKQSRRADGPFVVLNCAALAPDRVDVELFGCEPGWL
                     APEQPRVIGLLERADGGTLLLDEVADMPLDTQGKVLRALQERSFTRPGGNNRREVDVR
                     VLATTNRDLQAEMTAGRFREDLFYRINVVPVELPSLAEHRTDIPELARHFMTVASAES
                     GLQPHEIADDAMAVLQTADWPGDIRQLRNTIEWMLIMAGEQKGPIGLAGLPPELTDSA
                     ARGMDPRANGEFVSMPLREAREQFERTYLQAQLQRFGGNISRTANFVGMERSALHRKL
                     KTLGLHSDD"
     gene            14537..15913
                     /gene="trkA"
                     /locus_tag="P5558_09790"
     CDS             14537..15913
                     /gene="trkA"
                     /locus_tag="P5558_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRY24642.1"
                     /translation="MKVIVAGAGLVGFNIARYLAAAGNDVVIIDQRPELVRKIGDSLD
                     IQAMVGHASHPSTLEHAGAADADMFIAVTQVDEVNMVACQVAHTLFNVPTKIARVRQQ
                     DYLQSRWQDLFRRDHLPIDVVISPEVEVAHAIALRLEVPGALNVVPFAEDRVRLIALR
                     CTEATPIINTPLRQLTYLFPDLHLVCVGVARGDQFFIPHADDQLLPDDEVHFVVETAH
                     LERALPAFGYEERMSDRIIIVGGGNIGLFLARELEGRYPHLSIKVIENNPERAERIAD
                     ELKRSVVLCGDARDRTLLDEANVRSAQAIVTVTNDDEVNIMAALLGKRLGCERALALV
                     NNSTYNLVVRLIGLDVAINPRETTVSSILQHVRRGRLKSVHTIRDGEAEIYEAEALET
                     SPLVGKPLKDFRQSGGMIVGAIIRDDKVIMPRGDTVVQAHDRVVMVARSDVVRKVEQL
                     FAVRLDYF"
     gene            15954..16814
                     /locus_tag="P5558_09795"
     CDS             15954..16814
                     /locus_tag="P5558_09795"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HRY24643.1"
                     /translation="MARIAYVNGRYLPIGRPAVAIEDRGYQFADGVYEVIKCVFGQPR
                     DLERHLDRLERSLRELRIAMPMSRAALRRVIAETLRRNAIRHAIVYIQVSRGTAPRNH
                     LFPKNAVPSLVVTVRRAPFPKPAEIEDGVDVVTLPDQRWKRCDIKSISLLPNVLAKQQ
                     AAEQGAREAWLVDADGRVTEGSSANAYIVDQSGTIITRPLGGEILGGITRQNLFAAAK
                     AAGVPIVERAFTVAEARAAREAFLTSTSSLVLPVTRIDGQSIGNGKPGSITCRLLDAY
                     LQAQGLAAVI"
     gene            17001..17276
                     /gene="hfq"
                     /locus_tag="P5558_09800"
     CDS             17001..17276
                     /gene="hfq"
                     /locus_tag="P5558_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRY24644.1"
                     /translation="MAAEKQQNLQDVFLNHVRKNKIPVTVFLINGVKLQGVISSFDNF
                     CLLLRRDGHVQLVYKHAVSTVMPSAAVRLYDPAAADEAAREHAAERG"
     gene            17257..18588
                     /gene="hflX"
                     /locus_tag="P5558_09805"
     CDS             17257..18588
                     /gene="hflX"
                     /locus_tag="P5558_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRY24645.1"
                     /translation="MPPSEAETAIHEDSSATAGERPRAIVVHPHVTGRTQRSPAMRLD
                     EAVGLAAAIDLDIVAAEVAPLRDVRPATLFGGGRIEELKARIEELEASLVVVDGSLSP
                     IQQRNLERAWKCKVIDRTGLILEIFGERARTREGVLQVELAALTYQRSRLVRSWTHLE
                     RQRGGAGFMGGPGERQIELDRRRIDERITALKRELAQVTRTRGLHRAARQRVPYPIVA
                     LVGYTNAGKSTLFNRLTGAEVLAEDKLFATLDPTMRVVRLPSGRRVILSDTVGFISDL
                     PTTLVAAFRATLEEVLEADLLVHVRDITHEETESQAQDVHRVLDELGVDEAERRRRLI
                     EGWNKIDLAGEGERNLLLQQAAGRPEVVPFSALKGDGLDELLGCIEERLAAADEIVHL
                     DLDHRQGAIVAWLHQQGRVLQRIDDDSGIHLAVALPKAARGQLDRRLAEGL"
     gene            18727..>19124
                     /locus_tag="P5558_09810"
     CDS             18727..>19124
                     /locus_tag="P5558_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY24646.1"
                     /translation="MTTMRQILAKKGTKVWSITPDDTVLAGLERMAEADVGAMVVLEG
                     RKPVGIFNERRYAREVILKGRSSASTLVREIMQTDFKRVGPDQTVEECMALMTESRQR
                     HLLVMNRGRLLGIVSIGDLVKSIIDEQEHT"
BASE COUNT         3090 a         6503 c         6659 g         2872 t
ORIGIN      
        1 gccgagatcg agaccgacaa ggcgacgatg gaggtcgagg cggtcgacga gggcacgctc
       61 ggcaagatcc ttatcgacga gggcagcgag ggcgtcgccg tcaacacgcc gatcgccctc
      121 ctgctcggcg acggcgagga caagtcggcc ctcgatggct atgcagccgg cggcaatgat
      181 gctgccgagg cagcaccgca ggccgaggcg gaggagcagc ccgccgagca ggcgaagacg
      241 gagccgcagc ccaggcggca ggctctgccg accgagcccg tgcagccgga ggagcccgag
      301 gtgccggcgg gcaccgagat ggtcaagacg acggtgcgcg aggcgctccg ggatgccatg
      361 gccgaggaga tgcgcaggaa cggcgacgtg ttcctgatgg gcgaggaggt cgccgagtac
      421 cagggcgcct acaagatcag ccagggcctg ctcgaggagt tcggcccgag gcgcgtgatc
      481 gacacgccga tcacggaata cggcttcacc gggctcggcg tcggtgcggc catggcaggg
      541 ctccgcccga tcgtcgagtt catgacctgg aacttcgcca tgcaggcgat cgaccacatc
      601 atcaactccg ccgccaagac gctctacatg gccggtggcc agatgggcag cccgatcgtc
      661 tttcgcgggc ccaacggggc cgccgcccgc gtcgccgccc agcacagcca ggaatacagc
      721 tcctggtaca gccactgccc gggcctcaag gtcgtctgcc cctacagcgc ggccgacgcc
      781 aagggtctgc tcaaggcggc gatccgcgat cccaacccgg tggtcttcct cgagaacgag
      841 gtgctctacg gccagacctt cgacgtgccg aagctcgacg acttcgtcct gccgatcggc
      901 aaggccaaga tcgtccggcc gggcagcgac gtcaccatcg tgtcgttctc gctgatgatg
      961 acccgggccc tcgaggccgc cgaggcgctg gccgaggagg gcatctcggc cgaggtgatc
     1021 gatctgcgga ccatccggcc gctcgaccgg gcgaccgtgg tgaggtcggt gaagaagacc
     1081 aaccggctgg tcagcctcga ggaaggctgg ccgcagagcg gcatcggctc cgagctggcc
     1141 gcggtgatga tggaggacgc gttcgactat ctggacgcgc cggtcattcg cgtcaccggc
     1201 aaggacgtgc cgatgcccta tgccgcgaac ctggagcgca tggcgctgcc gcagaccaag
     1261 gacgtcgtcg aggcggccag ggccgtctgc tacaagagct agaagaacgc cggggaggtc
     1321 cacgatgcca atcaagatcc tgatgccggc gctctcgccg accatgaccg agggcaacct
     1381 cgcgagctgg ctgaagaagg agggcgacag cgtcgccccg ggcgacgtca tcgccgagat
     1441 cgagaccgac aaggcgacga tggaggtcga ggccgtcgac gagggccggc tcggcaagat
     1501 cgtcgtcccg gccggcaccg agggggtcgc cgtgaatgcc gtgatcggcc tcctgctcga
     1561 ggagggcgag gaggaatcag cactcgaggg tgcagccgac ggcggcgcgg atggcggcaa
     1621 tggcgaggcc aaggtggcgc cgcaaggggc gccgccggca gatccggcca gggccgaatc
     1681 gccccggacg gaggggccga gggaaggggc cgagaaggcg gccgccgggc cggcgccggc
     1741 tggaggctcc ggcaacggca gcggcgagcg catcttcgcc agcccgctcg cccgacgcat
     1801 ggccgagaag gccggtctcg atctggcggc gctctcgggc agcgggccgc gcggccggat
     1861 cgtcaaggcc gacatcgagg cggccctgga gaagggcggc gccgatgccg ccccggccaa
     1921 ggagaaggcg gcggagaagc cagcggcagc accagcacca gccccggcgg cggccggcgc
     1981 cccggacgcg ccgcatacgg agatcgccct ctccaacatg cgcaaggtga tcgcccgccg
     2041 gctcaccgaa tcgaagcagc agatcccgca tttctacctc acggtcgact gcgagctcga
     2101 tgccctgctc aagctccgga aggacctgaa cgagcggccg ggtgccgact acaagctctc
     2161 ggtcaacgac ctgctcatca aggcctcggc catcgccatc cggcagaagc cgacggtcaa
     2221 ctcgatctgg ggcggcgaca agctctacca gctcagcgat atcgacgttt cggtggcggt
     2281 ggccatcgag ggcggcctga tcacgccgat catcaggaag gcggaccaga aggggctggc
     2341 gacgatctcg gccgagatga aggatctcgc gacccgggcc aaggcgggca agctgaagcc
     2401 cgaggagttc cagggcggcg gcttctcgct ctccaatctc ggcatgttcg gcatcaagga
     2461 attctcggcg gtcatcaacc cgccccaggc cagcatcctc gcggtcggcg ccggggagca
     2521 gcggcccgtg gtcaagaacg gtgccttggc catcgccacc gtgatgagcg tgaccctgtc
     2581 ggtcgatcac cgggtcgtcg acggggcctt gggcgcggaa tggctccagg cgttcaagaa
     2641 gctcgtcgag gatccgatca gcctgatgct ctaggcaagt ggcggcagca ggcgttcgag
     2701 cacggggcga ttcgtcgggt cgaaagggtt gatcacgcgg agaccctcga gcaccgcgcc
     2761 gttctgcatg tcctcgctca ggaggtgcgt ggcgccggcg accgcggcgc aaatacaaat
     2821 cagcgcatcc cagaattgga tcttgtgacg ctcggtcagc cgtgcggcct tggccattct
     2881 gtcgccggtc gacgacggaa cgagcatggt cgaaggtatc gtctcgacga gctccagggc
     2941 gtcgatgtag cagtccggcc ggcggcggcg cacaacccag aggaactcgc cgagcacttg
     3001 cgcggggatc acggcaccgg ccaggccagc ccggtccaag agctcgactg ccgcgagccc
     3061 cttgatgctc tccggctcca gatgggcgta gatcaggaca ttggagtcga aggacgcgag
     3121 cattcagtcc tcgatcgggc cgtatttgtc ttcctcggtg atgttcccga cccggtagcc
     3181 gtcgcccttc cgggcgtgca gtagagccga ccaccgctcg tacgccgttc gcacctcggg
     3241 atcatccagc cggttgagcg gccgcgggct cagttgcgcc acgggcttgc cctggcgggt
     3301 gatcaccacg gtctcgccgg cctcgacctc cttgatcagc cgggagaact gctggttggc
     3361 atcacgaatg gagacggtgc gcatcttcca acctccatgt aggactgttc tacatatgcg
     3421 gcacgggcct ccggttgaca agttgggcga acccgccaca accaatcgac gtgccgggaa
     3481 cgccacttcg gggaccaacg ccatggccga cacctcattc gacatcgtca tcgtcggcgg
     3541 cgggccgggt ggctatgtcg ccgccattcg ggccgcccag ctcggcctga aaacggcggt
     3601 ggtcgagcgc gagcatctgg gcgggatctg cctcaactgg ggctgcatcc cgaccaaggc
     3661 attgctgcgc tcggccgaga tctacgacta tttcggccat gcggaatcgt tcgggctttc
     3721 ggtcgagaag atcggcttcg acctcgccaa ggtcgtcaag cggtcgcgcg gcgtggcgag
     3781 ccagctcagc aagggggtgg cgcatctcct gaagaagaac aaggtcaccg tcttcgacgg
     3841 cgccgggtcg ctcgccggca agggcaagct cgccgtcaag gcgacgaagg gcgacgacgt
     3901 cacgctggac gcgaagcaca tcgtcctcgc caccggtgcg cgggcgcgcc agctcccggg
     3961 gctcgagacc gacggcaagc tggtctggag ctacaaggag gcgatggtgc cggacgtcat
     4021 gccgaagtcg ctgctggtgg tcggcagcgg ggcgatcggc atcgagttcg cgagcttctt
     4081 ccgctcgatg ggggcggagg tgacggtggt cgaggtgctc gaccgggtgc tgccggtcga
     4141 ggatgcggac atctccgcct tcgccaagaa gcagttcgag aagcgcggca tcaagtgcct
     4201 gaccaaggcg caggtgaagg gcctgaagaa gggcaaggac aatgtcacgg ccaccatcga
     4261 ggccggcggc aagagccagg agctgaccgt cgatcgggtc atcatggccg tcggcatcgt
     4321 cggcaatgtc gagaatctcg gcctcgacag gacggcggtg aaggtggatc gggcgcacat
     4381 cgtcatcgac gagtggtgcc ggaccggcga gcccggcgtc tatgcgattg gtgatgtggc
     4441 cggcccgccc tggctggcgc acaaggcgag ccacgagggt gtcatgtgcg tcgagcggat
     4501 cgccggcgtg aagggcctgc acccgatgga tgtgacgaag atcccgggct gcacctactg
     4561 caacccgcag gtcgcctcgg tcggcctgac cgaggcggcg gccaaggaga agggccacaa
     4621 ggtcaaggtc ggcaggttcc cgttcatcgg caacggcaag gccattgcac tcggcgagcc
     4681 cgagggcatg gtcaagaccg tcttcgacgc cgagaccggc gagctcctgg gggcgcacat
     4741 gatcggggcc gaggtgaccg agctgatcca gggctacgtc atcgcccggc aggtcgaggc
     4801 gaccgagctc gacctgatgc acaccatctt cccgcatccg accctctcgg agatgatgca
     4861 cgagtcggtc cttgacgcct atggtcgtgc catccacttc tgaacggtcg aggacaggtc
     4921 aggatatcat ggcgacagtg acgttgacgc ggccgatcac ccgcaagccc gcctggatca
     4981 gggtgaaggc accgacctcg gcgagctacc acgagaccag gaagctgatg cgcgacaagc
     5041 gcctgcacac ggtctgcgag gaggcggcct gcccgaacat cggcgagtgc tggacgcaga
     5101 agcacgcgac ggtgatgatc ctgggcgaca cctgcacgcg cgcctgcgcc ttctgcaacg
     5161 tcaagaccgg cctgccgcgg gcggtcgacc cggaggagcc ggcgcatctg gccgaggtcg
     5221 tcctcaccat ggggctccgc catgtggtca tcacctcggt cgatcgcgac gacctgccgg
     5281 acggcggcgc cttccagttc gtgcgctgca tcgaggccat tcgggcggct tcgcccctga
     5341 ccacgatcga gatcctgacg cccgacttcc tgcgcaagcc cggggcggtc gagaaggtcg
     5401 tggcggcgag gcccgacgtc tacaaccaca atctcgagac ggtgccccgg ctctaccgca
     5461 ccgtgcggcc gggggcgcgc tatttccatt cgctgcgcct gctgcagcag gtcaaggagc
     5521 tggatcccgg catcttcacc aaatccgggg tcatggtcgg gctcggcgag gagaagcgcg
     5581 agatcctcca ggtgatggac gacatgcgct cggccgaggt cgatttcctc accgtcggcc
     5641 agtatttgca gccgacgccg cggcaccacg aggtcgtgcg cttcgtgccg ccggaggaat
     5701 ttgccggcat cgagcgggcg gcacgggcaa agggcttcct catggtctcg gccagcccgc
     5761 tcacgcgctc gtcgcaccat gccggcgccg atttcgagcg tctgcgcgag gcccgccggg
     5821 cacgggcggc cgcctgacgt cgccatgccg acccatgccg agcagcgcca tctgccctat
     5881 cggccggagc agctctacga gctggtcgcg ggcgtcgagc gctatccgga attcctgccc
     5941 tggtgcaagg cgtcgcgcat cacgcggcgg gaaggcgacg tgttctgggc ggacctggtc
     6001 atcgccttca aggtctttcg cgagcgcttc acctcgaagg tgacgctgat cccgaagacc
     6061 ggtgtcgatg tcgagtacat caacgggccg ttccgctatc tcaacaacca ctggcgcttc
     6121 ctgtcggtac ccgagggtgg ctgcatcgtc gacttctatg tcgatttcga gttccgctcg
     6181 aagatgctgc agagcctgat cggcctgctt ttcgaccagg cggtgcatcg catggtggcg
     6241 gccttcgaga cccgggccga tcagctctac ggcaagggca aggcggcgga cgacgggctg
     6301 gaaacggccg ttcactcgcc gagctgacgg ggtcagccga tcgccgcccg ttccagcagg
     6361 gctagcgccc gctcgagggc agcggtgcgg acggcacggc gatcacccgg aaagacatgg
     6421 cgctcgacct cggctgcgcc accccggcgc tgggcgccga tgaaaaccag gccgacgggc
     6481 ttggtcgctg ttgcaccgcc ggggccggcg atgccggtga cggcgatggc gagatccgcc
     6541 ggggagcggg cgagggcgcc tctcaccatc tcggccgcgg tctcggcgga gaccgcgccg
     6601 tggtgctcga gcgtggcggc ggcgacgccg agcatctccg tcttggcggc gttggagtag
     6661 gtgacgaagc cgcggtcgac cacgtcggag gagccggcga tctcggtcag gcaggcggcg
     6721 atgagcccgc cggtgcagct ttccgcggtg gcgagcgtca ggccgcgctc ccggcagcgg
     6781 gcgagcaggc gggcggcggt cttcagcgcc gactcgggca gggccccggt cacaggggaa
     6841 agctcaggct gacggtggct tgggcggcga ggccctcctc gcgaccgcag aagccgaggc
     6901 gctcggtggt cgtcgccttg acgctcaccc gacccgggtc gagcgccagc aggcccgcca
     6961 ggctcgcccg caaggtgtcg cgatgcgggc cgatcttcgg ccgctcgccg atgatggtga
     7021 gatcgacatg ctcgatccgg ccgccccggg cccgaacgag cccggtcgcg tgatgcagga
     7081 aatgcgcgct ggcgacgtcc cgccagcgcg gatcggacgg cgggaaatgg ctgccgatgt
     7141 cgccctcggc gatgccgccg agaagggcgt cggtgaccgc atggagggcg acgtcggcat
     7201 ccgaatggcc ggcgaggccg cggtcgtgcg gcagggtgat gccgcagagc acgagcgggc
     7261 ggcccggggc gaaggcatgc acgtcgaagc cggagccgct gatccagcgg cgcggctgtc
     7321 gggcgaggag ggcttcggct cgtgccatgt cggcctcggt cgtcaccttg aagttctcct
     7381 cctcgcccgc cacggccagc accggcacgc cgtcgagggc gacgacgccg gcatcgtcgg
     7441 tgcagtcccg atcggcatgg cgacgatggg ccaggagcag cgtggcgaaa tcgaagccct
     7501 ggggggtctg cgcccggccg agcccgtcac gcggcatctc ggccaccaca tcgccattcg
     7561 cgtcgacccg cttcagcgtg tcgaccacgg gcagcaccgg cagcaccgcc ctggcgctgg
     7621 cgagaccctg ccgcacccgg cccacgaggg caggcgaggc gaaggggcgg gcggcgtcgt
     7681 ggaccagcac atgggtcggc gcccgtcggg ccagcgcctc gaggccgcgc cgcacgctgg
     7741 cctgccgcgt ggcgccgccg atcaccggcg gcggcagctc gagcccccgc gtcgccgcgt
     7801 catagaggtc gtcctgcccg gccccgatca ccacctgcag ctcgcgcaat tccgggcagg
     7861 cacgcagggc gtcgatggtg cgtcgcagca tcggcagccc ggcaagcgcc tgatactgct
     7921 tgggtcgctc gcgcccgagc cgcagcccgc tacccgcggc catgaccaac gccacacact
     7981 ccatcagcct tcgcccgctc cggcaatgtc agggggaatg cagtttatgt ccgccgacgc
     8041 atgctggcca gcgcgcgggg ccattgtgcg gttgcaccat atcccgtaga tcgtgcactg
     8101 cagataggca gccttgcccg tctgcccaaa aaacggtcat tgcgggaaat gctgatgaca
     8161 gccatgcaga gcggcttttc gccaccggcc ccggcgcatc gccctgcggt cgacccgctg
     8221 gtcgtgctcg agaacctgcc gagcccggtg ctggtgctcg ccgggcgcgt gctggtcttc
     8281 gccaaccctg ccgccgagca gctcttctcg ctcgggcgct cggtgctgct cgggcgatcg
     8341 ctggacgatc tcctcgcacc acacagcacg ctcgccgccc tgatcggcca gctcgaggag
     8401 ggcgccggct cgatatcgga gtatggcata gagcttgctt tgcaacgggg cgtggtggtc
     8461 gcggtcgatg cgcatatcac gcccgttccg gaagcccagg atcacatctt gctcgtgctt
     8521 cacccatgct ccatggcgca gcggctcgat cgcattccgg cgggccgcag caatggccgg
     8581 tcgatcgccg ggctggccga gacgctggcg cacgaggtca agaacccact ctccggcatt
     8641 cgcggcgccg cccagctcct cgcaccgtcg ctcggcgagg aggaccgctc gctggtgcag
     8701 ctcatctgcg acgagaccga ccgcatctgc gccctggtcg accggatgga cgccttcagc
     8761 gagcccggcc gcatcgatcg ccggccggtc aacatccatc tcgtgctcga gcatgtgcgg
     8821 cgcctggcgc agaacggctt cgcccggcat ctgcgcatcg tcgagcacta cgacccgtcc
     8881 ttgccggacg tcgagggtga ccgcgaccag ctgatccagg tctttctcaa cctggtcaag
     8941 aacgccgccg aggcggcacc cgccgagggc ggccagatca ccctttccac cctgtaccag
     9001 cacggctttc ggatgagcgt cggggcggcg ggcgagcggc acgagctgcc gatcacggtc
     9061 gaggtgcgcg acaccgggcc cggcgtcgcg cccgacatga tcgatcatct cttccagcct
     9121 ttcgtcacca gcaaggccaa gggcagcggg ctcgggctct cgctcgtggc gaagatcgtc
     9181 ggcgagcatg gcgggctggt ttcctacacg cgcgcgcagc ccggctcgat cttcaaggtt
     9241 cgactgccga cggcacgcac gatccaaggg gtggggccga cgtgagcggc gagacggtgc
     9301 tgatcgcgga ggacgacgcg gccattcgaa cggtcgtctc ccgggcactc tcgcgccagg
     9361 ggttccacgt ccaggcgacc agcgtcgcgg cgaccctctg gcggtggatc gcggcggggg
     9421 agggcgatgt cgtgatcacc gatgtcggcc tgccggacga gaacagcctc gatctcctgc
     9481 cgcggatcaa gcagttgcgg ccggatctgc cgatcatcgt catgagtgcc cgcaacaccc
     9541 tcctgacggc ggtgagggcg accgagcggg gcgcctacga gtacctgccc aagcctttcg
     9601 acctgagcaa tctcctcgcc accgtgcagc gctctctcga ccggcgtgcg agccgcccgc
     9661 agcgtgccga ggagcccggc ctgcacgagg agcagttgcc gatcatcggt cgggcgccgg
     9721 cgatgcagga gatctatcgc gtcatcgccc gcctcgccgc caccgatctc accgtgctga
     9781 tcgccgggga atcgggcacc ggcaaggagc tcgtcgccag ggccctgcac gatttcggca
     9841 agcggcgcga cgggccgttc gtgccggtga atatggcggc catcccgcgc gagctgatcg
     9901 agagcgagct cttcgggcac gagaagggcg ccttcaccgg cgccggtgcg cgccgggccg
     9961 gccgcttcga gcaggcggaa ggcggcacgc tcttcctcga cgagatcggc gacatgccga
    10021 tcgaggcgca gacccggctc ctgcgggtgc tgcagcaggg cgagttcatc cccgtgggcg
    10081 gcagcaaccc gatccgcgcc aatgtccgga tcgtcgccgc gagccaccgc gacttcaggc
    10141 agatggtgag ccagggcctg tttcgcgagg acctctatta tcgcctcaac gtcgtgccgc
    10201 tgcgcctgcc ggcgctgcgc gagcgggccg acgacgtgcc ggccctggtc cagcatttcc
    10261 tcggcaaggc ccacgaggag gggctgccgg tcaagaccat cgatcccgac gcgatgcgcc
    10321 ggctgaagga gcacaactgg ccgggcaatg tgcgcgagct cgagaacctg gtccgccggc
    10381 tggccgtgct ctgcagcgag gagatcatcg gcgagagcgt gatccagagc gagctcgagg
    10441 acagccgcag gccggtcgag ggcgagccgg tggacgagac cctgagcggc gccgtcgcgc
    10501 gccatgtcga ccgctacttc gccgcccacg acgacgagct gccaccgccc gggctctatg
    10561 cgcgcatcct cgaggagctc gagcggccgc tgatcacccg gaccctgcag gcgacccggg
    10621 gcaaccaatt gcgcgccgcc gacatcctgg gcttgaaccg caacacgctt cgcaagaaga
    10681 taagggaact ggacatccat ctcgtccgca gcccctgacc cggccccctt gtaccggctg
    10741 gcgtccgggc gggcctgcca cccggagcgt cttggacaca ccctccccaa gctcactgag
    10801 cagtctgatc gccggcctgc gcggcgtgga tccctccgtg ctccatcggc tgcgccgctt
    10861 cgatttcgag cgcaagctgg cgctgctgct gtcgatcctc gccgtcgtct cgggcatcgc
    10921 cacctatgtc gtgatctcga cggccccgcc ctatgggcga agcgtgcaga cggtcgtcct
    10981 gctgctcaac gtcgacctca tcctgctcct gctgctcggc gtcatcatcg gccgccggct
    11041 ggtcatgctg gtcctgcagc accggcaggg ccgggccggc tccaagctgc atggccggct
    11101 ggtggcgctc ttcgccgtgg tcgccgtggc gccggcgatc atcgtctcgg tgttctccgt
    11161 gctgttcctg agctccggcc tcgagacctg gttcggcgac cggatcaggg tggcgctcga
    11221 gacctcgatg aacgtcgccg aggcctatct cgaggagcac aaggagaaca tccgcgccga
    11281 cgccctggcg atggccgccg acctcaaccg cgaggcgccc tatctgctcg gcgatcccgt
    11341 ccggctccag cagttcgtcg cgggccaggc ggccgtgcgc tcgctcacgg aagccatcgt
    11401 gttcgaccgc ctcggcaacg tcctcgcccg caccggcctc agcttcggcc tcgagatgga
    11461 gtatctgccg cccggtgcgc tgacccgggc ggagtccggc gaggtcgtca ccgtcacctc
    11521 gtcggccgac gaccgggtcc gggcactggt ccagctcttc ggcttcggcg acgcctatct
    11581 cttcgtcggc cggttcgtcg atgccgaggt gctgggcttc atgcagcgca cccagagcgt
    11641 ggtgcgcgag taccagcagc tctcgaccga gcggcgcggc atccagatca cctcggccat
    11701 cctcttcggc ctcgtcgccc tgctgctgct gttcgccgcc gtctggaccg gcatggcggt
    11761 cgccgaccgg atcgtggcac cgatcggcca gctcatcggc gccgccgacc gggtgcggac
    11821 gggcgacctg ctcgcgcgcg tccccgaggg ggcgagcgac gacgagatcg ccgtgctcag
    11881 ccgggccttc aaccgcatgg cgacgcagct gcacagccag cggcgcgagc tgatcgacgc
    11941 caaccaccag ctcgacgcgc gccgccggtt cacggaggcc gtgctgtccg gcgtcacggc
    12001 cggcgtgatc ggtctcgatg accagcggcg gatcgtcctg ccgaaccgtg ccgccagcca
    12061 tttcctcgag tccgccgccg gcggcgagat cgccggtgtc gacatccagc atgtcctgcc
    12121 cgagacgctg ccgctgttcg agcgggccga gctcgccgcc ggccagacgg tccagcagca
    12181 ggtgacggtg cggcgcggcc tcgccgagta cacgctcctg gtgcgtctcg cggcccagcg
    12241 cgagggtgac gcgattgtcg gctatgtcct gaccttcgat gacatcacgg tgctgctcag
    12301 cgcccagcgg caggcggcct gggcggaggt ggcgcggcgc atagcccacg agatcaagaa
    12361 cccgctgaca ccgatccagc tttcggccga gcgcatgggg cgcaagttcg cgagccagat
    12421 cgacgaggcc gaccggtcgt ccttccatcg cgcggtcgac acgatcatcc gccaggtcga
    12481 cgtcatcggc cggctgatcc gcgagttctc ggccttcgcc cggatgcccg acgcccgcat
    12541 gcgacccgag ccgctcgccg cgctcgtgca gcatgcggcg gacctgcagc agagcgcccg
    12601 tccggagctt cgcctgacgg tcgatctcgg cgattctgcc gggctctatc tcgactgcga
    12661 cggcgagaag atcagccagg cgctgaccaa tgtcctgcag aacgcggtcc aggcggtcga
    12721 ggagtcggcg gccggggccg atcaggaggg aggcggtagc ccggcgcccg tgcccgtggt
    12781 agaggtcacg gtgttgcgcg gcgaccacga gctggccatc ctggtcgccg acaatggcag
    12841 cggtttcccg gctggcgagc ggcagcggtt tctcgagccc tatgtcacca ccagggccaa
    12901 gggcacgggc ttggggcttg cgatcgtgca caagatcatg gaggagcacg ctggccgcgt
    12961 ggagctcgac aacagcgagc tcggtggcgc gctggtcagg ttggtttttc cggcggaacg
    13021 gatcgtcgag ggccggccac gacccatcgg caaccgggca tcttgagcgg gggttggcca
    13081 gcccttggag cccgctcggg cggggcctca gtggagacgg gcgaagcgga tgagcaagga
    13141 agttctgatc gtcgatgacg aggctgcgat ccgcgaggtc gtcgccgccg tcctcgagga
    13201 cgagggttat gtgccgcgcc aggccgcgac cagcgacgag gcgctccgcg aggtggcccg
    13261 caagccgccg gccttgctgc tgctcgacat ctggctcgag ggcagccgca tggacggcgt
    13321 gcagatcctc gaccatgtgc gctccagcaa cccgagcctg ccggtgatca tgttcagcgg
    13381 ccacgggacc atcgagaccg cggtggcggc gatcaagaag ggtgccctcg acttcatcga
    13441 gaagcccttc aagtcggacc ggctgctggt cgccgtcgag cgggccatgg agctgagccg
    13501 gctgcggcgg gagaacgagg agctgcgcag ccgggcccag aaggtcgagc tgatcggcgg
    13561 ctcgcccgtg atgggcaagc tgcgcgccac catccagcgg gtcggcccga ccggcagccg
    13621 catcctgatc tccggcccgc ccggctcggg caaggaggtc gtggcccgga tgatccacaa
    13681 gcagtcgcgc cgcgccgacg ggccgttcgt tgtcctcaat tgcgccgccc tggcgccgga
    13741 ccgcgtcgat gtcgagctct tcggctgcga gcccggctgg ctggcgcccg agcagccgcg
    13801 ggtgatcggc ctcctggagc gggccgatgg cggcacgctg ctgctcgacg aggtcgccga
    13861 catgccgctc gacacccagg gcaaggtgct gcgggcgctg caggaacgca gcttcacccg
    13921 gccgggcggc aacaatcgcc gggaggtcga cgtccgcgtc ctcgccacca ccaaccgcga
    13981 cctccaggcg gagatgacgg ccggccgttt tcgcgaggat ctcttctacc ggatcaacgt
    14041 ggtgcccgtg gagctgccgt ccctggccga gcaccgcacc gacatcccgg agctcgcccg
    14101 ccatttcatg acggtcgcct ccgccgaatc ggggctgcag ccgcacgaga tcgccgacga
    14161 tgccatggcg gtcctgcaga ccgccgactg gccgggcgac atccgccagc tgcgcaacac
    14221 catcgagtgg atgctgatca tggccggcga gcagaagggg ccgatcggcc tcgccgggct
    14281 gccgccggag ctcaccgaca gcgccgccag gggcatggac cccagggcca acggggagtt
    14341 cgtctcgatg ccgctgcgcg aggcgcgcga gcagttcgag cgcacctatc tgcaggcgca
    14401 gctgcagcgg ttcggcggca acatctcgcg caccgccaat ttcgtcggca tggaacggtc
    14461 ggcgctgcac cgcaagctga agacgctcgg ccttcacagc gacgactgag cgaagcagcg
    14521 gaggcgaggg cgcagcatga aggtgatcgt cgccggggcc ggcctcgtcg gcttcaacat
    14581 cgcgcgttac ctggcggcag ccggcaacga tgtcgtgatc atcgaccagc ggccggagct
    14641 ggtccgcaag atcggcgaca gcctcgacat ccaggccatg gtcggtcacg cctcgcatcc
    14701 ctcgaccctg gagcatgccg gtgccgccga tgcggacatg ttcatcgcgg tgacgcaggt
    14761 cgacgaggtc aacatggtgg cctgccaggt cgcgcacacg ctcttcaacg tgccgaccaa
    14821 gatcgcccgc gtccgccagc aggattatct gcagagccgc tggcaggatc tcttccgccg
    14881 cgatcacctg ccgatcgacg tggtcatctc gccggaggtc gaggtggcgc atgccattgc
    14941 cctgcggctc gaggtgccgg gtgcgctgaa tgtcgtgccc ttcgccgagg atcgggtgcg
    15001 gctcatcgcg ctccgctgca ccgaggccac gccgatcatc aacacgccgc tgcgccagct
    15061 cacctatctc ttccccgacc tgcacctcgt ctgcgtcggc gtggcccgcg gcgaccagtt
    15121 cttcattccc catgccgacg accagctcct gcccgacgac gaggtgcatt tcgtcgtcga
    15181 gacggcgcat ctcgagcggg cgctgccggc cttcggctac gaagagcgca tgagcgaccg
    15241 gatcatcatc gtcggtggcg gcaatatcgg cctttttctg gcccgggagc tcgaaggccg
    15301 gtatccgcat ctctcgatca aggtcatcga gaacaacccc gagcgggcgg agcggatcgc
    15361 cgacgagctg aagcgcagcg tcgtgctctg cggcgacgcc agggaccgga ccctgctcga
    15421 cgaggccaat gtccggagcg cccaggccat cgtcacggtg accaatgacg acgaggtcaa
    15481 catcatggcg gccctgctcg gcaagcggct cggctgcgag cgggcgctgg cgctggtcaa
    15541 caacagcacc tacaatctgg tcgtccggct catcggcctc gacgtcgcga tcaatccccg
    15601 cgagacgacg gtctccagca tcctccagca tgtccgccgg ggccggctca agtccgtgca
    15661 cacgatccgc gacggcgagg ccgagatcta cgaggccgag gcgctcgaga cgtcgccgct
    15721 ggtcggcaag ccgctcaagg atttccggca gtcgggcggg atgatcgtcg gtgccatcat
    15781 ccgcgacgac aaggtcatca tgccgcgcgg cgacacggtg gtgcaggcgc acgaccgggt
    15841 cgtcatggtg gcgcgcagcg acgtggtgcg gaaggtcgag cagctcttcg ccgtgcgact
    15901 ggactacttc tgacttcaac cgtgctggca cggtcagcga ggaaaggccc gagatggcgc
    15961 ggatcgccta tgtgaacgga cgctatttgc cgatcggccg cccggccgtg gccatcgagg
    16021 accggggcta ccagttcgcc gatggcgtct acgaggtcat caagtgcgtg ttcggccagc
    16081 cgcgcgacct cgagcggcat ctcgaccggc tcgagcggtc gctccgcgag ctgcgcatcg
    16141 ccatgccgat gtcacgggcc gcgctccgcc gggtcattgc cgagacgctg cggcgaaacg
    16201 ccatccgcca tgccatcgtc tacatccagg tcagccgcgg cacggcgccg cgcaaccacc
    16261 tctttcccaa gaacgcggtg ccgagcctgg tcgtcaccgt ccgccgggca ccctttccga
    16321 agccggccga gatcgaggac ggggtggacg tcgtcacgct cccggaccag cgctggaaac
    16381 gctgcgatat caagtcgata agcctgttgc cgaatgtgct ggcgaagcag caggcggcgg
    16441 agcagggggc ccgcgaggcc tggctggtgg atgccgatgg tcgcgtcacc gagggcagct
    16501 cggccaatgc ctatatcgtc gaccagtccg gcaccatcat cacccggccg ctgggcgggg
    16561 agatcctggg cggcatcacc cggcagaacc tcttcgccgc ggcgaaggcg gccggtgtgc
    16621 cgatcgtgga gcgggcgttc accgtggccg aggcgcgggc cgcgcgcgag gcgttcctga
    16681 cgagcacgtc gagcctcgtc ctgccggtga cccggatcga cggacagagc atcggcaacg
    16741 gcaagcccgg ctcgatcacc tgccgtctgc tcgacgccta tcttcaggcg cagggcctcg
    16801 ccgccgtcat ttgattgtcg catcgggcaa aaaaacactc tggcgattct atctggggct
    16861 agcaagggtc ggcccctgat cctaagcaat tgacgtgtgt gtgtgtgtga cgacggtgcg
    16921 cttgatcatc cgttctgccg accgacattc tggaggcggg gtgctcgtgc aaccaagaaa
    16981 acgacagagg gactcactcg atggcggccg aaaaacagca aaaccttcag gacgttttcc
    17041 tcaaccatgt ccggaagaac aagattcccg tcacggtttt cctgatcaat ggtgtcaagc
    17101 tgcagggcgt gatcagctcg ttcgacaatt tctgcctgtt gctgcggcgc gatggccatg
    17161 tccagctcgt ctacaagcat gcggtgtcga cggtgatgcc gtcggcggcg gtgcgtctct
    17221 acgatcccgc ggcggccgac gaggcggccc gcgagcatgc cgccgagcga ggctgagacg
    17281 gctattcacg aggattcttc ggcgacggcg ggggaacggc cgcgggcgat cgtcgtgcac
    17341 cctcatgtca cggggcgcac ccagcggtcg ccggcgatgc ggctggacga ggcggtcggc
    17401 ctggctgccg cgatcgacct cgacatcgtg gccgcggagg tggcgccgtt gcgcgacgtc
    17461 cgccccgcga ccctgttcgg tggtggccgg atcgaggagc tgaaggcgag gatcgaggag
    17521 ctcgaggcga gcctcgtcgt cgtcgatggc agcctctcgc cgatccagca gcgcaacctc
    17581 gagcgggcct ggaagtgcaa ggtcatcgac cgcaccggcc tgatcctcga gatcttcggc
    17641 gagcgggccc ggaccaggga aggcgttctg caggtcgagc tggccgccct gacctatcag
    17701 cgctcgcggc tggtgcgttc ctggacccac ctcgagcggc agcgtggtgg cgccggcttc
    17761 atgggtggcc cgggcgagag gcagatcgag ctcgatcgcc ggcgcatcga cgagcggatc
    17821 acggcgctca agcgcgagct ggcgcaggtg acgcgcaccc gcggcctgca ccgggccgcc
    17881 cgccagcggg tgccctatcc catcgtcgcc ctcgtcggct acaccaatgc cggcaagtcg
    17941 accctgttca accgcctgac cggtgccgag gtgctggccg aggacaagct cttcgccacg
    18001 ctcgacccga ccatgcgggt ggtgcggctg ccctcgggcc ggcgggtcat cctctccgac
    18061 acggtgggct tcatcagcga cctgccgacc acactggtcg ccgccttccg cgccacgctc
    18121 gaggaggtgc tcgaggccga cctgctcgtc catgtgcgcg acatcaccca cgaggagacc
    18181 gagtcgcagg cgcaggatgt ccatcgcgtg ctggacgagc tcggcgtcga cgaagccgag
    18241 cgccgccggc gtctgatcga gggctggaac aagatcgatc tcgccggcga gggcgaacgc
    18301 aacctcctgc tgcagcaggc cgccggccgg cccgaggtgg tgccgttctc cgccctcaag
    18361 ggtgacggcc tcgacgagct gctcggctgc atcgaggagc gcctcgcggc cgcggacgag
    18421 atcgtccatc tcgacctcga ccatcgccag ggtgccatcg tcgcctggct gcaccagcag
    18481 ggccgggtgc tccaacgcat cgacgacgac agcggcatcc atctcgccgt cgccctgccg
    18541 aaagcggcgc gcggccagct cgaccggcgg ctcgcggaag gtctctgacg ccgccgtcac
    18601 gacggttttt cgcgccggac gccggcttgc ctttccggct gcttcggggc agcatgccac
    18661 atggcagccg tcctcgatgg gcgggccagg cacgatggga tgtcgctgac cccgggggag
    18721 agcggtatga cgacgatgcg gcagatcctg gcaaagaagg gcaccaaggt ctggagcatc
    18781 acgccggacg atacggtgct cgcagggctc gagcggatgg ccgaggccga tgtcggggcc
    18841 atggtcgtgc tcgaggggcg caagcccgtg ggcatcttca acgagcgccg ctacgcgcgc
    18901 gaggtcatcc tcaagggcag gtcgtcggcg agcaccctgg tgcgggagat catgcagacc
    18961 gacttcaagc gggtcggccc cgaccagacg gtcgaggagt gcatggcgct gatgaccgag
    19021 agccgccagc gccatctcct ggtcatgaac cgggggcggc tgctcggcat cgtctcgatc
    19081 ggcgacctcg tcaagtcgat catcgacgag caggagcaca cgat
//
LOCUS       DAOTFI010000078        19007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3484_length_19007_cov_7.376266, whole genome shotgun sequence.
ACCESSION   DAOTFI010000078 DAOTFI010000000
VERSION     DAOTFI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 19007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19007
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3484_length_19007_cov_7.376266"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="P5558_09815"
     CDS             <1..880
                     /locus_tag="P5558_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRY24647.1"
                     /translation="KRDGTLWVKASGFWLADATKSDLFVPVDTARVLDAIDGGDEGDV
                     RAATIADKNPAGLRPSIETSLHALLPHRFVVHTHSVRTIAIAIRKDAEARFAERLQGL
                     SWAFVPYVFPGLPLTRAIRDAIDSRPIDVIVMGNHGLVVAADDLDAAAALLGEVERRL
                     DAPTLKLESAAPPSRAGLRPVRHAAANALAHHARLRERAAAGSYYPDHVIFLGPAAAT
                     PASCRPGQHLLLVPDEGAYLAEDAQPAADELALCLALVLARVPDDAELIRFTAAEEAA
                     LLGWDAEKYRQSLTRL"
     gene            complement(885..1172)
                     /locus_tag="P5558_09820"
     CDS             complement(885..1172)
                     /locus_tag="P5558_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24648.1"
                     /translation="MEMPMQSLLQPFVAAVLGIAGLALSLLLLPIVLALVLAIGLIAA
                     GGMAYARWRLRRELAGMEREAGPAPAGAARQTGQRARGRVIEADYEVVRER"
     gene            1291..2943
                     /locus_tag="P5558_09825"
     CDS             1291..2943
                     /locus_tag="P5558_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY24649.1"
                     /translation="MNGIDYIAEILKQEGVEWLSCFPSNPLISAAARIGIRPVAFRHE
                     RGAVMAADGYSRVSDRQRFGVVALQSQAGAENAMGGIAQANADNIPMLVFLGGNALDR
                     LSVRPNFTAARNYEGWVKRVEAIYTPDQVADVMRRAFHALRNGTPGPVVVELTVDVCA
                     REVPEAAQTYKSPRLVRQVPENAGIEAAADALIGARRGLIWAGAGVLFSGGTAALREL
                     AELTATPVFCTMPGKSAFDERHPLALGAGSGTTTGPAHQWLTESDVLLALGSSLTRTP
                     YGQKVHPGKTLIHNTINPDDLNKDEAADIGLVGDTRLTIEALIACIREKTKGLGFGDR
                     STTEAAIASAREKWLAEWNPILASDEEPISYYRVIQALNDTLDLENSIVTHDAGAPRD
                     VIVPFYTATTPHSYVGWGKTTHLGFGIPLMIGAKMAHPEKFCLNLMGDGAFGMSGTDI
                     ETAARSGAAITTVLLNNSAMATYSGPTQGNIGAEARERYGVSTMHGDYAKIAEAMGAT
                     GISVTRPAELEPALKEAQRLNATGQTVLIDVRANIEARRSRF"
     gene            complement(3059..3397)
                     /locus_tag="P5558_09830"
     CDS             complement(3059..3397)
                     /locus_tag="P5558_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24650.1"
                     /translation="MTVRSSGAPSAFVAADVGEDGACENVSEHIRVLIRRDEERLEQA
                     AFERLKAELGQAFAAPEASCRSQTLLAIRSGKVEGVSRTRIPDSKKVGRHAFPPAHPV
                     FSFRCSPVWW"
     gene            complement(4123..4812)
                     /locus_tag="P5558_09835"
     CDS             complement(4123..4812)
                     /locus_tag="P5558_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24651.1"
                     /translation="MAARDLDVLELYGESARLILAEPGKMLGLSAIGLAPSWIASLMS
                     DSFLLSLVVLVLSLPLVALAHGGLVVLVAQLRGGGPAPGFRGLLSGPNDGYVRITTAL
                     VVISVILGVGFVLLLVPGLIALAFLWVAWPLVVLEDAPAMAAIKESSRLTAGHRLKIT
                     AIVMILIVLNLALGLVQATLTAAFGLLFGLGVGALIGTATTALALTLVTLSYFKLKDP
                     TPAEPPQLSIH"
     gene            complement(4847..5578)
                     /locus_tag="P5558_09840"
     CDS             complement(4847..5578)
                     /locus_tag="P5558_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24652.1"
                     /translation="MTGRLAGKKALITAAGQGIGRASAEQFRDAGAEVIATDRDHGLL
                     ADLAGCRTAALDVTDKAAILALAKELGAVDVLLNCAGFVHNGTILECDDEAWEFSNRL
                     NVTAMFHMCQAFLPAMLDNGGGSIVNVASVAGSIKGVVNRFVYGTTKAAVIGLTKSIA
                     ADYVTRGIRCNAICPGTVETPSLDQRLHAQGDYAKARAAFIARQPMGRIGKPGEIAAL
                     AVYLASDESAYTTGQALIIDGGMVM"
     gene            5771..6631
                     /locus_tag="P5558_09845"
     CDS             5771..6631
                     /locus_tag="P5558_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY24653.1"
                     /translation="MPAFPIVDTHVHLWDPERIPLAWGDQVPTINRRHLIPEYDAARG
                     GVEVEAMVFLECDVAPGRHLDEARMVGEIAREEPRLRAIVAHAPLENGEEVAADLDVL
                     AREPRLRGIRRLLQQEADIEAVLDDRFLAGLGLLPRYNLHFEICVRHHQLEAIVDLVR
                     RLPNVTFMLDHIAKPAIQDARLEPWKTRMAELAALPNLMCKMSGLVTEADHQSWQPAD
                     LRPYIDHVVETFGFDRLVFGSDWPVMTLAADIPGWVAVLDEALAGASDSELRMFWLEN
                     AQAFYRLADG"
     gene            6674..8428
                     /locus_tag="P5558_09850"
     CDS             6674..8428
                     /locus_tag="P5558_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRY24654.1"
                     /translation="MLQLLRPRWGTRKAGAGADPARSRASHPGLSILVERGHLGPEAI
                     ERALVRAAETRQPLPVVLSELELLTGHDWAALMAERYGLEQAGPAELPTAPLLPETFA
                     ARFLRQAWVLPLAMDETTLWLGMADPADEAALKAARLAAERDVVPVVVPVEDLREAYS
                     RLFDSGQRALQEIVDDLALPGLDDASNVEQLIDLAKEAPVVRLVNQLLTDALRMGASD
                     IHIEPYPDRLQVRYRIHGRLKEIGAPPAKLAAAVISRIKILAKLDIAERRLPQDGRSQ
                     LTLLDRRIDLRVATMPTIHGESLVIRLLDQAAGSVELGHVGLAPGNEAALRRQLQSPH
                     GMILVTGPTGSGKTTTLYGALRTLDAASDKIISIEDPIEYQIDGVTQIQVKPEIDLSF
                     ARILRSVVRHDPNVVMVGETRDAETAEIAVHAALTGHLMLTTLHTNSAAGAVARLLDM
                     GVEPYLLASVLRCVIGQRLVGVLCDACKEAYVPSVDERHLFENRGIELPTPLRLCRPV
                     GCRACNEIGFTGRIGIFELLEVDEPVRELIRDRAPSQSLQAHALGRGMTTMFQDGLAK
                     ALAGTTTLEEVCRVTEEW"
     gene            8457..9683
                     /locus_tag="P5558_09855"
     CDS             8457..9683
                     /locus_tag="P5558_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRY24655.1"
                     /translation="MPQYAYRALTEAGERVAGEIEAGDRRGAIQRLQAQGLIPIEAQP
                     AGRGALPGIEAPAGRPARSGGRQAAAVTVATRELATLIGAGETLESALALVAGELADR
                     RLRAVFEGILNRVRTGASLSDAMAAEPRIFSRLYVGMVRAAEATGRLGEVLRELADLR
                     ERQEALRRQLTSALIYPTVLMLTAIGAVLVLLLYVVPQFTPVFAGHEDRLPPLTRFIL
                     DAAAWLRTHGPTVAVVLASLGLLWLLASQIGSLRLLGHRLTLRLWGIGTLARERATAE
                     VCRGLATLLKGGLDLPSALLLMREMVSNLAIAEALGRAASAVRQGRRLSEALATEGIL
                     QPMGQKLLKTAEESGRLEPLSRYIAERFEQILATRMQRFVTLLEPLLVIGLGGMVGGI
                     VIAILTAVLSVNELAF"
     gene            9845..10345
                     /locus_tag="P5558_09860"
     CDS             9845..10345
                     /locus_tag="P5558_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24656.1"
                     /translation="MAVLGVLALAWLWWTGDDFQTGMASDDPAGTVPVAHAEPVAVPE
                     VRPVADGALFARALPSIEAFSGMLERPLFSSTRRPAVVAPELALMDVPSAAVEPAAGL
                     RPPAIRFIGSVEENGAVRAFVGDGLDVQALATGDEIEGWRVVSIEPRRLLLGLDDERY
                     ELKILE"
     gene            10354..10794
                     /gene="gspG"
                     /locus_tag="P5558_09865"
     CDS             10354..10794
                     /gene="gspG"
                     /locus_tag="P5558_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRY24657.1"
                     /translation="MRAKSTGFGHREDGFTLIELLVVLVILGLLAAVAGPRVIGYLGG
                     ARSDTAGVQVAAFRQALDLYRLDVGDYPTTEQGLEALIEAPSSATGWNGPYLDGQTVP
                     LDPWGNEYVYRVPGADDAYDLLSLGADNRQGGSGEAADIGAGTN"
     gene            10760..11164
                     /locus_tag="P5558_09870"
     CDS             10760..11164
                     /locus_tag="P5558_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY24658.1"
                     /translation="MRLPTSARAPTDAGFTLLELLVTLTLLAVVAATILPRFGGERPR
                     TLEQRAGEIARELGRMRLEAVGSGRVLTVPTTDLAAGLPAGMTLAATETQELVFFPNG
                     MTNGAVWRLETEDRAVGLTVDWLTGRVAVDAP"
     gene            11154..11564
                     /locus_tag="P5558_09875"
     CDS             11154..11564
                     /locus_tag="P5558_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRY24659.1"
                     /translation="MRHDEAALRDEGFTLLEVLVALAVLGVVLTTLFRLAGDSLVQYS
                     GREARFRLALTAEAAMEIERLTPGEARRNVWPADIELTVERRRFAAVAGAWSGLAATL
                     PALGEELEWLVVRAEDGAGRSFTLEAAVPSGRAP"
     gene            11561..12226
                     /locus_tag="P5558_09880"
     CDS             11561..12226
                     /locus_tag="P5558_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY24660.1"
                     /translation="MSPHGAGGERGFTLVEMVVALVLLGLVMALIGAGARVLRGTGDR
                     LTERGDSLADLALFSTLVQERLGDAVQLDFGPAGLPVAAFDGNAARVRFLTLAPDFQP
                     GAPLLAMEIGSEPETGIWLGLAPLAADQKDFAVLDDAGLTERRVLVADATSLRISYFG
                     RQADADPAWHEVWQDEERLPRAVRFDLDHARLALPPVIVAVRQQVATLCASAVAELVC
                     PEP"
     gene            12253..13179
                     /locus_tag="P5558_09885"
     CDS             12253..13179
                     /locus_tag="P5558_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRY24661.1"
                     /translation="MALLTVLWFVAAMSALAITLVSISRDTAYVTRNAVRAIEARAAL
                     QGAVEIAGRLLSVNGFPATGVLEWTNDGGNIRITAVPEGGKIDLNAADEALIEGLASA
                     VAEDSGTAQSLADALLDWRDQDDARRLAGAELDDYARDGGTVRPRDGPLPFAAELRSV
                     QGFDLDLYARLADAVTVFPGNTEPGPVPLAPLVREALDYATGLAVEGIDDRESGESAF
                     DPPPESLEDEPNDAPATAFAEDPQGLYTLDIELAYDDGPSFRQKTVIWVEPPLGSDPF
                     LVLQNGAGLVPRAAASTAASETIAGGQPWRSR"
     gene            13164..14279
                     /locus_tag="P5558_09890"
     CDS             13164..14279
                     /locus_tag="P5558_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRY24662.1"
                     /translation="MAVALSGQPGQRGGGFFAWWADELAGLMPANGRRLPTDRFVVAV
                     FDGRTLQFALRHRGRVEQLGTVESMPEDRESDTGAALLADRINRRELPLVLRLVPDLG
                     IQTADRLPVAAKGELRSIIANKLDMLTPWSAETACFDTVRVGTADGDMLAIEIAVAPE
                     KTVARAVDAVERLGLVVDVVDLGEDDPYAPPTHDLLHGNRPRRLPRWLPIGGALLAAA
                     AVTIGIFAYFDIEARNAGLAQRRAYASLLEQRLEDLPEIRGRIDALEAGSRAVLERHL
                     GRPSTLATIEKLSRLLPDTVWLDTLTIDGRRLTVTGYSGDAARLPALIEGNASFEAAR
                     LTAPSERRAVVNEVGDLVEVDQFSLQAEVRPGMEVPR"
     gene            14279..14872
                     /gene="gspM"
                     /locus_tag="P5558_09895"
     CDS             14279..14872
                     /gene="gspM"
                     /locus_tag="P5558_09895"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HRY24663.1"
                     /translation="MATLSPALSRWLAVLLLLVPLAGFAALAIVPARLAERQAVERET
                     LEAHIARLEQRIVTREQVLAELRQLERQTEGDQRLLRAETPSVGGAALAGLLTEFFEQ
                     SGGRLDRTQVLEPVVDDLVMRIGVQLSGSMQMAGLRSLLHRIETHEPLLTVERIAIQG
                     IDLGAGEVLVDTDLTVVGYARVPPSEESEGSSTDGAG"
     gene            14916..15281
                     /locus_tag="P5558_09900"
     CDS             14916..15281
                     /locus_tag="P5558_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HRY24664.1"
                     /translation="MSEVLYHRGEADDRPWGRWEVLDTGPGFCVKRLTVQPGGKLSLQ
                     LHHHRAEHWVIVAGTAHVTRGEEVLELGQDAHVYLPLGIKHRIENRGTVPVEFIEVQT
                     GDRLDENDIVRFEDNYGRP"
     gene            15321..16067
                     /locus_tag="P5558_09905"
     CDS             15321..16067
                     /locus_tag="P5558_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24665.1"
                     /translation="MAQVILVTGGSRGIGAACCRLASRRGYDVAINFQGNARAADAVA
                     DDVRAAGARALTIQADVSDAAAVERMFDTAAAELGPVAAFVSNAGVINKAAPLADIAV
                     EEIRRIVEIDLTAHLICNRAAVRRMARSRGGEGGAIVNISSMASSLFGAGGFIPYGAA
                     KGGIDVLTQGLGREVAGDGVRVNGIRPGLIDTDIQDDTGVENRIARFGSTVPIGRAGT
                     AAEVAEAVLWLLSDQAAYVTGTLFDVSGGR"
     gene            16082..16912
                     /locus_tag="P5558_09910"
     CDS             16082..16912
                     /locus_tag="P5558_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRY24666.1"
                     /translation="MALGPVLLALAILLAAPVSAAEIACGETAPCAVPGGTYHLAAPA
                     GWDGTTPLPAVLFFHGYGSSGAAVVGNSALRDSVTGRGYLLIAPDGREPEPGRRRSWA
                     HQGSPDSGRDENAFIGEVMADVAARVPLRRDGVLVSGFSQGGSMAWHVACSMGGDFAA
                     FAPVSGAFWRPHPEGCTGEPVRLLHLHGLADTVVPLEGRPIGERWHQGDVFEALAQLR
                     AEAGCGSQPDRISIEDGRRCRAWTSCAPGGELELCLHDGGHELPADWLGRALDWFEAG
                     "
     gene            17131..17571
                     /locus_tag="P5558_09915"
     CDS             17131..17571
                     /locus_tag="P5558_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24667.1"
                     /translation="MPGDETSAVEALTHAVLERLQPVDNAERELADGIIAALWRMRRA
                     TQIEREVLGLRPGGSEGDDPNAVARAFVRQSHGPRTLELLLRYRTQAVGELGRLLRLL
                     ADLRGAAVAQPPLVASARVEAAANDNRPAIAPSRCHPLAPGLDD"
     gene            complement(17568..18425)
                     /locus_tag="P5558_09920"
     CDS             complement(17568..18425)
                     /locus_tag="P5558_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY24668.1"
                     /translation="MKLYRDFEDQAALDAEYNPGQSVPDAAARMADWPVRGARALERL
                     RHQLDIRYGPTLAEYLNVFPAGPGSPVHVFVHGGYWRRFSARDFAWVAEPLVATGVTA
                     VIVNYALCPTVSLTEIVRQTRAAIAWCHLEIGRFGGDPARLTLSGHSAGGHLVGMALA
                     TDWQGQYGLPNDLVAGAMAVSGLFDLRPFPYTYLQPALQLTTAEVLALSPIDLEPTSS
                     CPLEIVVGGAESAEFRRQSRSFSDRWQALGADCRHGEIPNANHFTALDALFTDDSPLL
                     QRLLAYADG"
     gene            complement(18478..>19007)
                     /locus_tag="P5558_09925"
     CDS             complement(18478..>19007)
                     /locus_tag="P5558_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRY24669.1"
                     /translation="SLVQTIGRAARNIDGRCILYADTMTRSLKAAIEETDRRRAKQLA
                     YNEANGITPASIKRGIQDIMASAAERDYVTVDLGEDAPATLSGMDLQAYIGELEKKMR
                     DAAADLEFEEAARLRDEIKRLEDGELGLPTTRPARSGDRSDGRKPWQPSATHADKRRA
                     AEAKKRAGARRRSGA"
BASE COUNT         2821 a         6800 c         6530 g         2856 t
ORIGIN      
        1 caaaagggac ggcacgctct gggtcaaggc ctcgggcttc tggctcgccg acgccacgaa
       61 gagcgacctc ttcgtcccgg tcgacaccgc ccgcgtcctc gatgccatcg acggcggtga
      121 cgagggcgac gtgcgcgccg ccaccatcgc ggacaagaac cccgcgggac tccgcccctc
      181 catcgagacc agcctgcacg cgctcctgcc gcaccgcttc gtcgtccata cccactcggt
      241 ccggacgatc gccatcgcca tccgcaagga cgccgaggcc cgcttcgccg aacgcctgca
      301 gggcctgagc tgggccttcg ttccttacgt cttccccggc ctgccgctca cccgggcgat
      361 ccgcgacgcc atcgattcgc gccccatcga cgtcatcgtc atgggcaatc acggcctcgt
      421 cgtcgccgcc gacgatctcg acgcagcggc ggcactcctc ggcgaggtcg agcgccgcct
      481 cgacgcccca accctgaagc tcgaatcagc cgccccgccg tcgcgggcag gcctgcgccc
      541 cgtccgccac gccgcggcga acgccctggc gcaccacgcg cgcctgcgcg agcgggccgc
      601 cgccggctcc tactaccccg accatgtcat cttcctcggc cccgccgctg ccacgccggc
      661 cagctgccgc cccggtcagc acctcctcct cgtgccggac gaaggcgctt accttgccga
      721 ggacgcccag cccgccgccg acgagctggc cctctgcctc gccctcgtcc tggcccgcgt
      781 gcccgacgac gccgagctga tccgcttcac cgccgccgag gaagcggccc tcctcggctg
      841 ggacgccgag aagtatcgcc agtcgctgac gcggctctga ccgctcagcg ctcgcgcaca
      901 acctcgtaat ccgcctcgat cacccgcccg cgcgcccgct ggccagtctg ccgggccgcc
      961 ccggccggag ctggcccggc ctcgcgttcc atgcccgcca gctcgcgccg cagccgccag
     1021 cgcgcatatg ccatgccacc ggccgcgatc agcccgatcg cgagaaccag cgccagcacg
     1081 atcggcagca gcagcaggct caaggccagc cccgcgatcc ccagaacggc agccacgaag
     1141 ggctgcaaca gcgactgcat cggcatctcc atggtttccc cagaatatgg gatgcggatc
     1201 ggccgctgcg agacccggcg tcaccgtggt tgtcgctccg ccccggctgg tgcacgctgg
     1261 ctccatgatc gggcagcggg gggaacaagc atgaacggca tcgactacat cgcagaaatc
     1321 ctgaagcagg aaggcgtcga gtggctctcc tgcttcccga gcaacccctt gatctcggcc
     1381 gcggcccgca tcggcatccg cccggtggcc ttccgccacg agcgcggtgc cgtcatggcg
     1441 gccgacggct acagccgggt cagcgaccgg cagcgcttcg gcgtcgtggc cctgcagtcg
     1501 caggccgggg ccgagaacgc catgggcggc atcgcccagg ccaatgccga caacatcccc
     1561 atgctggtct tcctcggcgg caacgccctc gaccgcctct cggtcaggcc gaacttcacc
     1621 gcagcccgga actatgaggg ctgggtcaag cgcgtcgagg cgatctacac cccggaccag
     1681 gtcgccgacg tcatgcgccg cgccttccat gcgctccgca acggcacgcc cggcccggtc
     1741 gtcgtcgagc tgaccgtcga cgtctgcgcc cgcgaggtgc cggaagcggc acaaacctac
     1801 aagtcaccca ggctggtgcg ccaggtgccg gaaaatgccg ggatcgaagc cgccgccgac
     1861 gccctcatcg gcgccaggcg aggcctgatc tgggccggcg ccggcgtcct cttctccggc
     1921 ggaaccgccg cccttcgcga gctcgccgag ctgaccgcca cccccgtctt ctgcaccatg
     1981 ccgggcaagt ccgccttcga cgagcgccac ccgctggccc tcggcgccgg cagcggcacc
     2041 accaccggcc cggcccacca gtggctgacc gaaagcgacg tgctgctcgc cctcggcagc
     2101 agcctgaccc gcacccccta tggccagaag gtccaccccg gcaagaccct catccacaac
     2161 accatcaacc cggacgacct caacaaggac gaggccgccg atatcggcct cgtcggcgac
     2221 acccgcctca ccatcgaggc cctcatcgcc tgcatcaggg agaagaccaa gggcctgggc
     2281 ttcggcgacc gcagcaccac cgaagccgcc atcgccagcg ccagggagaa atggctcgcc
     2341 gaatggaacc cgatcctcgc ctccgacgag gagcccataa gctactaccg cgtgatccag
     2401 gccctcaacg acacgctcga cctcgagaac agcatcgtca cccacgacgc cggggcaccg
     2461 cgcgacgtca tcgtgccctt ctacaccgcc accacacccc acagctatgt cggctggggc
     2521 aagaccaccc acctgggctt cggcatcccg ctgatgatcg gcgccaagat ggcgcacccg
     2581 gagaagttct gcctcaacct catgggcgac ggcgccttcg gcatgtccgg caccgacatc
     2641 gaaaccgccg cccgctccgg tgccgccatc accaccgtcc tcctcaacaa cagcgccatg
     2701 gccacctact ccggccccac ccagggcaac atcggcgccg aagcccgaga acgctacggc
     2761 gtctcaacca tgcacggcga ctacgcgaaa atcgccgaag ccatgggtgc caccggcatc
     2821 tccgtcacca gaccagccga gctggaaccc gccctgaagg aagcccaacg cctcaacgcc
     2881 accggccaaa ccgtcctcat cgacgtccgc gccaatatcg aagcccggcg gtcgaggttc
     2941 tgaccaacga agcgggggaa atgatttccc ccggcacccc ctcgggactt tgggttgttg
     3001 gtcgagcccg ggtggtgcac gcttgcggaa agtcgggaca cgacactctt ttaacctgtc
     3061 accaccacac tggactgcac cgaaacgaaa agaccggatg ggccggtggg aaggcgtgcc
     3121 ttcccacctt cttggagtcc gggattcggg tcctcgagac gccttcaacc tttcccgatc
     3181 gaatggccaa gagggtctgg gaccgacagg acgcctctgg cgcggcaaag gcctggccga
     3241 gctccgcctt cagccgctcg aacgccgcct gctccagccg ctcctcgtca cgccggatca
     3301 ggacccggat gtgctcgctg acgttctcgc aagccccgtc ctctcccaca tccgccgcca
     3361 cgaacgcgct cggtgcgccg ctgctgcgaa cagtcatctt cattgtcgtg cgtgacacag
     3421 catgccctct tcggccccat ccgtcttcaa gatgaccaat atcgaataca tgacgatccg
     3481 atcgaggccg gagccggtgt cgccgctggc aggcaccaga ccttttcgac attccccggc
     3541 cggacgccgt tcgagccgca ctgatcgctg ccacgatgct tcctcaacat gccgacgctg
     3601 ccggccgcca tcgaagcgag ctcgtccacc gcgccgccat cacggatgct catagccgag
     3661 ccccgacgga cggccggaaa gcgtcgctcg gcgctcttcg gccctctccg gcgctcgggt
     3721 tggcgcatca gaagcgacat tggggctcgg gttgtcgcgg attctgcaac aggacctgcc
     3781 ggaatgtcgc ggatcccgcg acataacgat tcgtgatggc gcgcccaatg cgacattcgc
     3841 ccgggaatgt cgcggattcc gcaacacgag gccgcgaacg aacccgggaa aacgacgttc
     3901 gaacccacgg aatcgacgat cgaacccggc gattcgacga acgaacccag tcggcagcca
     3961 acgaacccac ggccgatcaa catactgatt caaagaagga ttatgccatt cagcgccctt
     4021 tggcgcctcg tcgagcaacc caagaaccga aaactgccga acgaaccgag gcggcgcttg
     4081 cggtcggggg cagttcgtga taggattatt atctgatcgc tctcagtgga tgctgagctg
     4141 tggcggctcg gctggggtcg ggtccttcag cttgaagtag ctgagggtga cgagggtcag
     4201 ggccagggct gtcgtggcgg tgccgatgag ggcgccgacg ccgaggccga agagcaggcc
     4261 gaaggcggcg gtcagcgtcg cctggacgag gccgagggcg agattgagga cgatgaggat
     4321 catgacgatg gccgtgatct tcagccggtg cccggcggtg agccggctgc tctccttgat
     4381 cgccgccatg gccggcgcgt cctcgaggac gacgagcggc caggcgaccc agaggaaggc
     4441 cagcgcgatc agccccggca cgaggagcag gacgaagccg acgccgagga tgacgctgat
     4501 gacgacgagg gcggtggtga tccggacata gccgtcgttc gggccggaga ggagccccct
     4561 gaagcccggc gctggcccgc cgccgcggag ctgcgccacc agcacgacga gcccgccatg
     4621 cgccagggca accagcggca ggctgagcac gaggacgacc agggacagga ggaaggagtc
     4681 gctcatcagg ctggcgatcc aggagggggc gaggccgatc gcgctcagcc ccagcatctt
     4741 gcccggctcg gcgaggatca gcctcgccga ttcgccatag agctcgagaa cgtccagatc
     4801 ccttgccgcc acgtgccgtc ctcccctgat cccggccgct tcgggcctac atcaccatgc
     4861 cgccgtcgat gatcagggcc tggcccgtcg tgtaggcgct ctcgtcgctg gcgaggtaga
     4921 cggcaagggc cgcgatctcc cccggcttgc cgatgcggcc catgggctgg cgggcgatga
     4981 aggcggctcg cgccttggcg tagtcgccct gcgcgtgcag ccgctggtcg agcgacggcg
     5041 tctccaccgt gcccgggcag atggcgttgc agcgaatgcc cctggtcacg tagtcggcgg
     5101 cgatcgactt ggtcagcccg atcacggcgg ccttggtggt gccgtagacg aagcggttga
     5161 cgacaccctt gatgctgccg gcgaccgagg cgacgttgac gatcgacccg ccgccattgt
     5221 ccagcatggc cggcaggaac gcctggcaca tgtggaacat ggcggtgacg ttgagccggt
     5281 tcgagaactc ccaggcctcg tcgtcgcatt ccaggatggt gccgttgtgg acgaagccgg
     5341 cgcagttcaa gagcacgtcg accgcgccca gctccttcgc gagcgccagg atcgccgcct
     5401 tgtcggtgac atccagcgcc gccgtgcggc agccggcgag gtccgcgagc agcccatgat
     5461 cgcggtcggt ggcgatcacc tcggcccccg catcgcggaa ctgctcggcc gaagcccggc
     5521 cgatgccctg tcccgcggcg gtgatcaacg ccttcttgcc ggccagcctt ccagtcacgt
     5581 cccgagccct ccccatcgtg cgacgccttg gcctaggcag cgccggacac gccgtcaagc
     5641 gacgtgacgg ccgagccgca tcgcggccgg gccggccgct ggcgctgcag ggccttcagc
     5701 agaggtagcc aagcgcgcat atccgactat cctgcctacg ttcgtcctca agccaatcga
     5761 gcagcacccc atgccggcat ttccgatcgt cgatacgcac gtccatctct gggaccccga
     5821 gcgcattccg ctcgcctggg gcgaccaggt tccgacgatc aaccggcggc acctgatccc
     5881 cgaatacgac gcggcgcggg gcggggtgga ggtcgaggcc atggtgttcc tcgagtgcga
     5941 cgtggcgccc ggccgccatc tcgacgaggc ccggatggtc ggcgagatcg cccgcgagga
     6001 gccaaggctc cgggccatcg tcgcccacgc gccgctcgag aacggcgagg aggtcgctgc
     6061 cgacctcgac gtgctggccc gcgagccgcg cctgcgcggc atccgccggc tgctgcagca
     6121 ggaggccgac atcgaggcgg tgctcgacga ccggttcctc gccggcctcg gcctcctgcc
     6181 gcgctacaac ctccacttcg agatctgcgt ccgccaccac cagctcgagg ccatcgtcga
     6241 cctggtccgg cggctgccga atgtgacgtt catgctcgac cacatcgcca agccggccat
     6301 ccaggacgcc cggctcgagc cctggaagac gcgcatggcc gagctcgccg ccctgcccaa
     6361 cctgatgtgc aagatgtcgg gcctcgtgac cgaggccgat caccagagct ggcagccggc
     6421 cgacctgcgg ccctatatcg atcacgtggt cgagaccttc ggcttcgatc gcctcgtttt
     6481 cggcagcgac tggccggtca tgacgctggc cgccgacatc cccggctggg tggcggtcct
     6541 cgacgaggcg ctggccggcg cttccgacag cgagctgcgc atgttctggc tggagaacgc
     6601 gcaggctttc taccgtctcg ccgacggctg accctccggt gcacgatccg ccactccatt
     6661 cgcggagaca gtgatgctgc agcttcttcg cccgcgctgg ggaaccagga aagcaggcgc
     6721 cggcgcggat cctgcccgca gccgggccag ccaccccggg ctctccatcc tcgtcgagcg
     6781 cggccatctc gggcccgagg cgatcgagcg cgccctcgtc cgggcggccg agacccgcca
     6841 gccgctgccc gtcgtgctga gcgagctcga gctgctcacc ggccacgact gggcagcgct
     6901 gatggccgag cgctacggtc tcgagcaggc cggcccggcc gagctgccga cagcgcccct
     6961 cttgcccgag acgttcgccg cgcgcttcct gcgccaggcc tgggtgctgc cgctcgccat
     7021 ggacgagacg acgctctggc tcggcatggc cgacccggcc gacgaggcgg cgctcaaggc
     7081 ggcgaggctc gccgccgagc gcgacgtggt gccggtggtc gtcccggtcg aggatctgcg
     7141 ggaggcctat tcacgcctct tcgacagcgg ccagcgggcg ctgcaggaga tcgtcgacga
     7201 tctggcgctc cccggcctcg acgatgcgtc gaacgtggag cagctcatcg acctcgccaa
     7261 ggaggcgccg gtcgtccgcc tggtcaacca gctcctgacc gacgcgctca ggatgggggc
     7321 ctccgacatc cacatcgagc cctatcccga tcgcctgcag gttcgctacc gcatccacgg
     7381 ccggctgaag gagatcggcg ccccgcccgc gaagctggcg gcggcggtga tctcgcgcat
     7441 caagatcctg gccaagctcg acatcgccga gcgccgcctg ccgcaggacg ggcgcagcca
     7501 gctcacgctc ctcgaccgcc ggatcgacct gcgtgtcgcc accatgccga ccatccacgg
     7561 cgagagcctg gtcatccgcc tgctcgacca ggcggcgggc tcggtcgagc tcggccatgt
     7621 cggcctcgcc cccggcaacg aggcggcgct gcgccggcaa ttgcagtcgc cgcacggcat
     7681 gatcctggtc acgggcccga ccggcagcgg caagacgacg acgctctacg gggcgctgcg
     7741 gaccctcgac gcagcgagcg acaagatcat cagcatcgag gacccgatcg agtaccagat
     7801 cgacggtgtc acgcagatcc aggtgaagcc ggagatcgat ctcagcttcg cccggatcct
     7861 gcgctcggtc gtgcgccacg acccgaacgt cgtcatggtc ggcgagacgc gcgatgccga
     7921 gaccgccgag atcgcggtgc acgcggcgct gaccgggcat ctgatgctga ccaccctgca
     7981 caccaacagc gccgccggcg ccgtcgcccg gctgctcgac atgggggtcg agccgtatct
     8041 cctggcctcg gtcctgcgtt gcgtcatcgg ccagcggctg gtcggcgtgc tctgcgacgc
     8101 ctgcaaggag gcctatgtgc cgagcgtgga cgagcggcat ctcttcgaga accgcggcat
     8161 cgagctgccg acgcccttgc gcctgtgccg gccggtcggc tgccgcgcct gcaacgagat
     8221 cggcttcacc ggccggatcg gcatcttcga gctgctcgag gtggacgagc cggtgcgcga
     8281 gctgatccgc gaccgggcgc cgagccagtc cctgcaggcc catgcgctgg gtcggggcat
     8341 gacgaccatg ttccaggacg gcctcgccaa ggcgctcgcc ggcacgacca cgctcgagga
     8401 agtctgccgg gtcaccgagg aatggtaggg cagggtctcg gccagggggt tcgcgcgtgc
     8461 cgcaatacgc ctaccgggcg ctgaccgaag ccggcgaacg tgtcgccggc gagatcgagg
     8521 ccggtgaccg ccggggcgcc atccagcggc tgcaggccca gggcctgatc ccgatcgagg
     8581 cgcagccggc cgggcgcggc gcgcttccgg gcattgaagc accggccggg cggccggcgc
     8641 ggagtggcgg tcggcaggca gcggcggtga ctgtcgccac gcgcgagctc gccaccctga
     8701 tcggtgccgg cgagaccctg gagagtgccc tggccctggt cgccggcgag ctcgccgacc
     8761 gacggctcag ggcggtcttc gaaggcatcc tcaaccgggt caggacgggt gcctcgctga
     8821 gcgacgccat ggcggcggag cccaggatct tctcgcggct ctatgtcggc atggtgcgcg
     8881 ccgccgaggc gaccgggcgg ctcggcgagg tgctgcgcga gctggccgat ctgcgcgagc
     8941 ggcaggaggc gctgcgccgg cagctcacct ccgcgctgat ctatccgacc gtgctgatgc
     9001 tgaccgcaat cggcgccgtc ctcgttctcc tgctctatgt cgtgccgcag ttcacacccg
     9061 tcttcgccgg ccacgaggac cggctgccgc cgctcacccg gttcattctc gatgccgccg
     9121 cctggctgcg gacgcatggc ccgaccgtgg ccgtggtgct ggcctctctc ggtctcctct
     9181 ggctgctcgc ctcgcagatc ggctcgctgc ggctgctcgg ccatcggctg accttgcggc
     9241 tctgggggat cggcaccctc gcccgcgagc gagccacggc cgaggtctgc cgaggtctcg
     9301 cgacgctgct caagggcggt ctcgacctgc cgtcggcgct cctgctcatg cgcgagatgg
     9361 tgagcaacct cgcgatcgcc gaggccctcg gccgtgctgc ctccgcggtc cgccagggac
     9421 ggcggctgag cgaggcgctg gcgaccgagg gcattctcca gccgatgggc cagaagctct
     9481 tgaagaccgc cgaggagagt ggtcggctcg agccgctctc ccgctacatc gcggaacgct
     9541 tcgagcagat cctggcgacg cggatgcaac gtttcgtcac gctgctcgag ccgctgctcg
     9601 tcatcggcct cggcggcatg gtcggcggca tcgtgatcgc catcctgacc gcggtgctga
     9661 gcgtgaacga gctcgccttc tagcctgcta ggtcgatgat cctccatcgg gccgcttggc
     9721 gccggggccg gaatccgcta gacacccgtt ggtcacagct gggcgccttc tcttggcgcc
     9781 acctcttcga cccctcagac agatcaaggc cccggtgcaa gcctggcatc ccatcaccaa
     9841 actcgtggcg gttctcggtg tcctcgccct ggcgtggctc tggtggaccg gcgacgattt
     9901 ccagaccggc atggcgagcg acgacccggc cggcacggtg cccgtcgccc acgccgagcc
     9961 cgtggccgtg cccgaggttc ggcccgtggc cgatggtgcg cttttcgccc gcgccctgcc
    10021 gtcgatcgag gcgttcagcg gcatgctcga gcggccgctg ttcagctcga cgcgtcggcc
    10081 ggcggtggtg gcgcccgagc tcgccttgat ggacgtgccg agtgctgcgg tcgagccggc
    10141 tgcggggctt cggccgcccg ccatccggtt catcggcagc gtcgaggaaa acggagccgt
    10201 cagggcgttc gtcggcgacg gcctcgatgt gcaggcgctc gcgacaggcg acgagatcga
    10261 aggctggcgc gtggtgtcga tcgagccgcg gcgactgctc ctcggcctcg acgacgagcg
    10321 gtacgagctg aagattctgg agtagcggtc aaattgcgag cgaaatccac tggtttcggt
    10381 catcgtgagg acggcttcac gctgatcgag ctgctcgtcg tgctggtcat cctcggcctg
    10441 ctcgctgcgg tcgccgggcc aagggtcatc ggctatctgg gtggtgcgcg gagcgatacg
    10501 gccggcgtgc aggtggcggc cttcaggcag gcgctcgatc tctaccggct cgacgtcggc
    10561 gactatccga cgaccgagca ggggctcgag gcgctgatcg aggcgccatc gagtgctacc
    10621 ggctggaacg gcccgtatct cgacggccag acagtgccgc tggatccttg gggcaatgag
    10681 tatgtctacc gcgtcccggg agcggacgac gcctacgatc tcctgtcgct cggcgccgac
    10741 aaccggcagg gcggcagcgg tgaggctgcc gacatcggcg cgggcaccaa ctgacgccgg
    10801 cttcacgctg ctcgagctgc tggtcacgct gaccctgctc gcggtggtgg ccgccaccat
    10861 cctgccgcgg ttcggcggcg aacggccacg gacgctcgag caacgggccg gggagatcgc
    10921 ccgcgagctc ggccgcatgc gcctcgaggc agtcggcagc ggccgggtgc tgaccgtccc
    10981 gaccaccgac ctggctgccg gcctgccggc cgggatgacg cttgccgcca ccgagacgca
    11041 ggagctcgtc ttctttccca acggcatgac caacggcgcc gtgtggcggc tcgagacgga
    11101 ggatcgcgcc gtgggcttga cggtcgactg gctgaccggc cgggtggcgg tcgatgcgcc
    11161 atgacgaggc cgccctgcgg gacgagggct tcaccctgct cgaggtgctg gtggcgctgg
    11221 ccgtgctcgg cgtcgtgctc accaccctct tccgcctcgc cggcgacagc ctcgtccagt
    11281 actcgggccg ggaggcgcgt tttcgcctgg cgctgaccgc cgaggcggcc atggagatcg
    11341 aacggctgac tccgggcgag gcgcggcgca acgtctggcc ggccgatatc gagctcactg
    11401 tggagcgccg ccgtttcgcc gcggtggccg gggcatggtc cggcctggcc gcgaccctcc
    11461 cggcgctcgg cgaggagctg gaatggctgg tggtccgcgc cgaggatggg gccggccgca
    11521 gcttcacgct cgaggcggcg gtgccgtccg gccgagcgcc atgagcccgc acggcgccgg
    11581 cggcgagcgc ggcttcacgc tggtcgagat ggtcgtggcg ctggtcttgc tcggcctcgt
    11641 catggccctg atcggcgccg gcgcccgggt gctgcggggt accggcgacc ggctgacgga
    11701 gcgcggcgac agtctggccg acctcgccct cttctcgacc ctggtgcagg agcgcctggg
    11761 cgatgcggtc cagctcgact tcggccccgc cgggctgcct gtcgccgcct tcgacggcaa
    11821 cgccgcacgt gtccgcttcc tgacccttgc cccggatttc cagcccggcg cgccgttgtt
    11881 ggcgatggag atcggctcgg agccggagac cggcatctgg ctcgggctgg cacccttggc
    11941 cgccgaccag aaggacttcg cggttctcga cgatgccggg ctgaccgagc ggcgcgtgct
    12001 cgtggctgat gcgacctcgc tcaggatcag ctatttcggg cggcaggcgg acgcggaccc
    12061 ggcctggcac gaggtctggc aggacgagga gcggctgccg cgcgccgtgc gcttcgacct
    12121 cgaccatgcg cggctggcgt tgccgccggt cattgtcgcg gtccggcagc aggtcgccac
    12181 gctctgtgct tccgccgtgg ccgagctcgt ctgtcccgag ccgtgaggcg ccccatcccc
    12241 gaagatgccg gcgtggcgct cctcaccgtg ctctggttcg tggcggcgat gagcgctctc
    12301 gccatcacgc tcgtctcgat cagtcgcgat accgcctatg tcacgcgcaa cgcggtgcgg
    12361 gcgatcgaag cacgcgctgc cctgcaaggt gcggtcgaga tcgccggccg actcctctcg
    12421 gtcaacggtt tcccggcgac cggcgtgctc gaatggacca atgacggtgg caacatccgg
    12481 atcacggcgg tccccgaagg cggcaagatc gacctgaatg ccgccgacga ggcgctgatc
    12541 gagggcctcg cttccgccgt ggccgaggat tccggcaccg cgcagtccct ggcggatgcc
    12601 ctgctcgact ggcgcgacca ggacgatgcc cgtcggctcg ccggtgccga gctggacgac
    12661 tatgcgcgag atggtggaac cgtccggccg cgtgacgggc ccttgccgtt cgccgccgag
    12721 ctgcgctcgg ttcagggctt cgacctcgac ctctatgcac gtctcgccga tgccgtcacc
    12781 gtgttccccg gcaataccga gcccgggccg gtgcccctcg caccgctcgt tcgcgaggcg
    12841 ctggactacg ccaccggtct cgctgtggaa gggatcgacg accgggaatc cggcgaaagc
    12901 gctttcgatc cgccgccgga gtcgctagag gatgagccga atgatgcgcc tgcgacggca
    12961 ttcgccgagg atccgcaagg cctttatacc ctggatatcg agttggccta cgacgatgga
    13021 ccgagcttca ggcagaagac ggtgatctgg gtcgagccgc cgctcggcag cgacccgttc
    13081 ctcgtcctgc agaacggcgc tggcctcgtg ccgcgcgccg cggcgagcac ggccgccagc
    13141 gagacgatcg ctggaggtca accgtggcgg tcgcgctgag cggccagccc gggcagcgcg
    13201 gcggtggctt cttcgcctgg tgggcggatg agctcgccgg gctcatgccg gccaatggcc
    13261 gccgcctgcc gaccgaccgc ttcgtggttg ccgtgttcga cggccgcacg ctgcagttcg
    13321 cgctgcgcca tcgcggccgc gtcgagcaac tgggcaccgt cgagagcatg cccgaggatc
    13381 gcgagtccga tacgggtgct gctctcctgg cggatcgcat caaccgtcgc gagctgccgc
    13441 tggtgctgcg cctcgtgccg gatctcggta tccagacggc ggatcggctg ccggtggcgg
    13501 ccaagggcga gcttcggtcg atcatcgcca acaagctcga catgctgaca ccctggtccg
    13561 ccgagacggc ctgcttcgac acggtccggg tcggcacggc agatggcgac atgctggcga
    13621 tcgagatcgc cgtggcgccg gaaaagacgg tggcccgggc ggtcgacgcc gtcgagcggc
    13681 tgggcctcgt ggtcgacgtg gtcgatctcg gcgaggacga cccctatgcg ccgccgaccc
    13741 acgacctgct gcacggcaac cggccgcgcc ggctgccgcg ctggctgccg atcggtggtg
    13801 ccctgctcgc cgcggcggcg gtgaccatcg gcatcttcgc ctatttcgat atcgaggccc
    13861 gcaacgcggg actggcgcag cgccgggcct atgccagcct gctggagcag cggctggagg
    13921 atctgcccga gatccgcggc cggatcgatg cgctggaggc cggcagccgg gcggtgctgg
    13981 aacggcacct cgggcggccg tcgaccctcg ccaccatcga gaagctgagc cggctcctgc
    14041 cggataccgt ctggctcgat acgctcacga tcgacgggcg gcgcctgacc gtgaccggct
    14101 atagcggcga cgcagccagg ctgccggcgc tgatcgaggg caatgcgtcc ttcgaggcag
    14161 cgcggctgac ggcaccgagc gagcgccgtg cggtggtgaa cgaggtgggt gacctcgtcg
    14221 aggtcgacca gttctcgctg caggccgagg tgcggccggg catggaggtg ccccgctgat
    14281 ggcgacgctc tcgcccgccc tgagccgctg gctcgccgtg ctcttgctgt tggtgccact
    14341 ggccggattc gcggcgctcg cgatcgtccc ggcccggctc gccgagcggc aggcggtgga
    14401 gcgtgagacg ctggaggcgc atatcgcccg gctcgagcag cggatcgtca cccgcgagca
    14461 ggtgctcgcc gagttgcgcc agctcgagcg ccagacggag ggcgatcagc ggctgctccg
    14521 cgccgagacg ccttccgtcg gcggcgcggc gctcgccggt ctgctcaccg agttcttcga
    14581 gcaatccggt ggccggctgg accgcactca ggtgctcgag ccggtcgtcg acgatctcgt
    14641 gatgcgcatc ggcgtgcagc tcagcggcag catgcagatg gccggcctgc gcagcctgct
    14701 gcaccgcatc gagacgcacg agccgctgct gaccgtcgag cggatcgcca tccagggcat
    14761 cgacctcggc gccggggagg tgctggtcga taccgatctg acggtggtgg gctatgcccg
    14821 agtgccgccg agcgaggaaa gcgaaggttc atcgaccgat ggcgccggct gaccggtcgc
    14881 cgccgtcttc atcaccaagg ggggggacac gtaagatgag tgaagtgctc taccatcgcg
    14941 gcgaggccga cgaccgaccc tggggtcgct gggaggtgct cgacaccggg cccggcttct
    15001 gcgtcaagcg cctcaccgtg cagccgggcg ggaagctgtc gctgcaactg caccaccatc
    15061 gcgccgagca ttgggtgatc gtcgccggca cggcccatgt gacgcgcggc gaggaggtgc
    15121 tggagctcgg ccaggacgcc cacgtctatc tgccgctcgg catcaagcac cgcatcgaga
    15181 accggggcac cgtgccggtc gagttcatcg aggtgcagac cggcgaccgg ctcgacgaga
    15241 acgacatcgt gcgtttcgag gacaattacg gtcgcccctg atccggggcg acttgctcca
    15301 ttcgactgag ggaacggata atggcccagg ttatcctcgt taccggcggc agccgcggca
    15361 tcggtgccgc ctgctgcagg ctcgcgtcgc gacgtggcta tgacgtcgcc atcaacttcc
    15421 aaggcaacgc aagggcggct gacgcggtcg ccgacgatgt gcgtgcggcc ggtgcgaggg
    15481 cgctcaccat ccaggccgac gtgagcgatg cggcagcggt cgagcggatg ttcgacaccg
    15541 cggcggccga gctcggcccg gtggctgcct tcgtcagcaa tgccggggtc atcaacaagg
    15601 cggcgccgct cgccgacatc gccgtcgagg agatccgccg gatcgtcgag atcgatctca
    15661 cggcgcacct gatctgcaac cgcgcggcgg tccgtcgcat ggcgaggtcg cggggcggcg
    15721 agggcggtgc catcgtcaac atttcgtcga tggcctcgag cctgttcggc gcgggcggct
    15781 tcatccccta tggcgccgcc aagggtggca tcgacgtgct gacccagggt ctgggcaggg
    15841 aggtggccgg tgacggtgtt cgcgtcaacg gcatccggcc aggcctgatc gacaccgaca
    15901 tccaggacga cacgggcgtc gagaaccgca tcgcacgttt cggctcgacc gtgccgatcg
    15961 gccgcgccgg caccgccgcc gaggtcgccg aggccgtcct ctggctcctc tcggaccaag
    16021 ccgcctacgt caccggcacg ctcttcgacg tatcgggcgg ccgctgacgg gcacttgtcg
    16081 aatggcgctc ggcccggtgc ttctggcgct cgccattctc ttggcggcac cggtgtcggc
    16141 cgccgagatc gcttgcggcg agacggcccc gtgcgcggtg ccgggcggca cctaccatct
    16201 ggcggcgccg gccggctggg acggcacgac gcccttgccg gccgtgctct tctttcacgg
    16261 ctacggctcc tccggggccg ccgtcgtcgg caattccgcc ctgcgcgatt ccgtcacggg
    16321 ccgcggctat ctgctgatcg cgccggacgg ccgcgagccg gagcccggcc ggcgccggag
    16381 ctgggcgcac cagggctcgc cggactcggg acgcgacgag aacgccttca tcggcgaggt
    16441 gatggcggat gttgcggcgc gggtgccgct ccgccgggat ggcgtgctgg tatccggctt
    16501 cagccagggc ggctcgatgg cctggcacgt cgcctgctcc atgggcggcg acttcgctgc
    16561 gttcgccccc gtctccggtg ccttctggcg gccgcatccc gagggctgca ccggcgagcc
    16621 ggtgcggctc ctgcatctgc atgggctcgc cgacacggtc gtgccgctcg agggccggcc
    16681 gatcggcgag cgctggcacc agggggacgt gttcgaggcg ctggcgcagt tgcgcgccga
    16741 ggctggctgc ggctcgcaac cggatcgcat ctccatcgag gacggccgtc gctgccgcgc
    16801 ctggacgagc tgcgcgccgg gtggcgagct cgagctctgc ctgcacgatg gcgggcacga
    16861 gctgcccgcc gattggctcg gccgggcgct cgactggttc gaggcgggct gaagatctcg
    16921 tttatgttga attaattcct acattcgact agattgacgg ctcccgatgc aacgaggtga
    16981 gccatgtccg accgcccgag aacgccggcg caaatcgccg caagccgcgc gaacgggcga
    17041 cggtcgaacg gacccaagac cgaggcgggc aagcggcgct cccgcctgaa cgccgtcaag
    17101 cacggtctcc ggtccaggac catccgcccg ttgccgggtg acgaaacctc cgccgtcgag
    17161 gccctgacgc atgccgttct cgagcgcctg cagccggtcg acaatgccga gcgggagctg
    17221 gccgacggca tcatcgcggc gctctggcgg atgcgtcggg cgacgcagat cgagcgcgag
    17281 gtgctgggcc tgcggcccgg cgggtccgag ggggacgatc ccaatgccgt cgcccgggcc
    17341 ttcgtccggc agagccacgg gccgcggacc ctggagctgc tgctccgcta tcggacccag
    17401 gcggtgggcg agctcggccg actgttgcgc ctcctcgccg acctgcgcgg cgctgcggta
    17461 gcgcagccgc cgttggtcgc ctcggccagg gtcgaggccg cggccaatga caaccggccg
    17521 gcgatcgcgc cctcgagatg ccatccgctc gctccaggct tggacgatta gccgtcggca
    17581 taagcgagca gccgctgtag cagcgggctg tcgtcggtga atagcgcgtc gagcgccgtg
    17641 aaatggttgg cgtttgggat ctcgccatgg cggcaatcgg ccccgagcgc ctgccagcga
    17701 tcgctgaagc tgcggctctg ccggcggaac tcggcactct ccgcgccacc gacgacgatc
    17761 tcgagcgggc aggacgacgt gggctcgagg tcgatcgggc tcaacgccag aacctcggcg
    17821 gtggtgagct gcagggccgg ctgcagatag gtgtagggaa aggggcgcag gtcgaagaga
    17881 ccggagacgg ccatcgcacc ggcgacgagg tcgttcggca ggccgtattg tccctgccag
    17941 tcggtggcga gcgccatgcc gaccagatgg ccgccggccg aatggcccga aagcgtgagg
    18001 cgcgccgggt cgccgccgaa ccggccgatc tcaaggtgac accaggcgat ggcggcacgg
    18061 gtctggcgga cgatctcggt gagcgagacg gtcgggcaca aggcgtagtt gacaatgaca
    18121 gcggtgacac cggtggccac cagcggctcc gccacccagg cgaagtcgcg ggccgagaag
    18181 cgccgccaat agccgccatg cacgaagaca tgcaccggcg agccgggccc ggccggaaac
    18241 acattcaggt attcggcgag agtgggacca tagcggatgt cgagctggtg ccgcagccgc
    18301 tccagcgccc gcgcgccgcg aaccggccag tccgccatgc gcgccgcggc gtcgggcacg
    18361 gactggcccg ggttgtactc ggcgtcgagc gccgcctggt cctcgaagtc gcgatagagc
    18421 ttcatccggc ccccctgctg ccctggggcc gcaacaaagg tggccggggc agcgccgtca
    18481 agcgccgctg cggcggcggg cgccggcgcg ctttttggcc tccgcggcgc gccgcttgtc
    18541 ggcgtgcgtg gccgaaggct gccagggctt gcggccgtcg gaccggtcgc ccgagcgcgc
    18601 cggccgtgtt gtcgggaggc cgagctcgcc gtcctccagc cgcttgatct cgtcgcgcag
    18661 ccgggccgcc tcctcgaact ccaggtcggc ggcggcgtcg cgcatcttct tctcgagctc
    18721 gccgatatag gcctgcaggt ccatgccgga gagggtcgcc ggcgcatcct cgccgagatc
    18781 gaccgtgaca tagtcgcgct cggcagcgct cgccatgatg tcctgaatgc cccgcttgat
    18841 gctcgccggg gtgatgccgt tggcctcgtt gtaggcgagc tgcttcgccc ggcggcggtc
    18901 ggtctcctcg atcgccgcct tgagcgagcg ggtcatggtg tcggcataga gaatgcagcg
    18961 cccgtcgatg ttgcgggcgg cgcggccgat ggtctgcacc agcgagg
//
LOCUS       DAOTFI010000079        18854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3520_length_18854_cov_8.193308, whole genome shotgun sequence.
ACCESSION   DAOTFI010000079 DAOTFI010000000
VERSION     DAOTFI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18854
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3520_length_18854_cov_8.193308"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            629..1255
                     /locus_tag="P5558_09930"
     CDS             629..1255
                     /locus_tag="P5558_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRY24670.1"
                     /translation="MPNAATPPELVLFDLGGVLIDWDPRRLYRKLFADEAAMEQFLST
                     VCTPAWNLELDRGRPFAVAVEELAALYPEERPLIEAYRQRWLEMIGGPIGETVTLLEE
                     LAARRTPLRALTNWSAETFTLVHPHPDYAFLERFAQIYVSGELRMIKPNDDIFEHVLE
                     DTGLAAESILFIDDSAANIRTAERLGFVTHRFAGADGLELHLRDLQLI"
     gene            1261..3690
                     /locus_tag="P5558_09935"
     CDS             1261..3690
                     /locus_tag="P5558_09935"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRY24671.1"
                     /translation="MGRLLATLAAVGFGLGLGAAALAGFLVWASLPMLEGEIRLAGLG
                     APARVSRDELGVPLIEAASERDAAMALGFVHAQDRLWQMEINRRIGAGRLAEVVGERG
                     LAVDRFMRTLGLYRAAEASLDHLSAEALDRLAAYAEGVNGFLAQRAGPLPPEFLLLRH
                     RPEPWRPADSLVLIKLMALDLVSSWRSELTRASLLGRLPPAALADLWPPPRPDEVTTL
                     PGAAQWAGGLPSGAGSTDPAAAIETAALAEALGAALPLADNAGLGSNIWAVAGAHTAS
                     GAALLANDPHLGLATPAHWYLAAMRTPAGTVLGATLPALPAVVIGRNEHIAWGFTNTA
                     GDTDDLFVERLDPADPDRYLTPDGSRPFLRRDEVIEVRGGEAVHLLVRETRHGPVVSD
                     IMTRAAEVAGEGRVLALAWTALLPEDRTIEAGIGLARARDWDGFTRAIALFHSPTQNA
                     FYADAEGRIGMVMAGRLPLRRAGDGSLPAPGWDGSHDWLGLLPAAENPATPGATAAGF
                     LQNANNRLVDDRYPHLVTADWNDDLRARRITAVLDAATAEGAPLDIDAMRRLQNDRLS
                     TLAADFRPRLEAVAPADAETALILAAIAAWDGAAMPDRPEPLVFQAWYRSLVRHVLAD
                     ELGEGFAAFRGIRSEAMRHILEQAPGWCDDVATADRTESCGDMATAALASSLDELRAT
                     YGADWRSWRWGEASRARLAHRPLDAAPGIGRLFSIVTGSGGGDAGTVHVARYAADMPY
                     ATVAAASLRLVADLARSGTLHAILPTGQSGHPLSPHFSDQTGLWRNGELHTLEIAQPV
                     GRGRSELVLRP"
     gene            3771..4397
                     /locus_tag="P5558_09940"
     CDS             3771..4397
                     /locus_tag="P5558_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY24672.1"
                     /translation="MQLSELRSFGQRLRSLRVGLELSQAELARRIGRHQTAIGPYERD
                     EYMPGRDVVEKLAAALETSPEYLQFGRMPYRSSLPQLGRLGASGQLTSPAHAGPLALA
                     DDRLAALTLEDEAMRPVFRAGQTVLVARLATTALEPLIGRHVLAELDDGRQFLRRLAP
                     AADSGNYVLDAYNAAPLGPLRPVAARLVLGHLEVEALSFRAQEPGASG"
     gene            4524..4952
                     /locus_tag="P5558_09945"
     CDS             4524..4952
                     /locus_tag="P5558_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY24673.1"
                     /translation="MNQVASKPVGKGVGGRSRALDVDHYVSLRIRQRRIMLGLTQQQM
                     AELIGVTYQQAHKYETGINRISAGRLYQIAQALGVDIGYFFDDLDPDKQSRPKSTEMM
                     PQQRMLLELARNFAAIGNRKHQDAICNLARVLASGERAES"
     gene            complement(4989..5420)
                     /locus_tag="P5558_09950"
     CDS             complement(4989..5420)
                     /locus_tag="P5558_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY24674.1"
                     /translation="MRRDFPDRPIIGVGVVVWRDDRVLLIRRGKPPRAGQWSLPGGAQ
                     ELGETVAETARREVSEETGLDLASLDLLTVVDLVEHTPDGARVLYHYTLIDFTAEAAP
                     GTAIAGGDAAAVGWFHAGELHGLGLWAETIRIIRLAATRRA"
     gene            complement(5453..6556)
                     /locus_tag="P5558_09955"
     CDS             complement(5453..6556)
                     /locus_tag="P5558_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRY24675.1"
                     /translation="MNGLVYAAGRPWPQLLRSVLDQLDGDAAPAPLGLVFLADPTTEM
                     ADIMLDGLRQRTGIQTWLGAASTAVFAGGRELIGEGAIAALPLPLEGVTLHPFAGSPP
                     TGAEEATCGILHVPDAADAGAAVAALAARTSAVLTGAQGSSSFDHAQIAGSPSAGAAT
                     GVLLSGNVTMVAGITHGCRPLGPAHRVTAGAGSTLSALDGRAATAWLAEEAGDLVLRD
                     QRQLRRQLVLAQVPTEVGLAGSSLAPADALFGIVHADPLRGSIEISGIGLGGGGLPSR
                     IQLFRRDPALALEHLQALAADLLQKLGGRPPRAALFYSSIERGIGFFGPGVDEATVLG
                     RVLGPVPVIGMRSAAEVAGTKLTRYAAALALIG"
     gene            complement(6835..7755)
                     /locus_tag="P5558_09960"
     CDS             complement(6835..7755)
                     /locus_tag="P5558_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY24676.1"
                     /translation="MNGAGWARGWGGAAKGGGRHAEDESYFVSVSDLMVGLLFLFIII
                     LMAFALNFRSAEDAAEETLDALTKERNVLELERDALESDQAALTLERDRLLADRDRLA
                     GIIGQLLARDALRAELLTLIRQALAARGLEVEVDVENGILRLPESLLFATNEAELSET
                     GRAAIGVLAEVLWRYLPCYAAMAEAEGCPTGVHDVLETVLIEGHTDDRPIRSGPFPDN
                     WALSAARSRSTYRAMIAAEPRLDDLRNGSAAALLGVSGYEARRPVADGATDEARRVNR
                     RIDVRFLSRGPDAAALAELLETLETSFVPR"
     gene            complement(7759..9129)
                     /locus_tag="P5558_09965"
     CDS             complement(7759..9129)
                     /locus_tag="P5558_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24677.1"
                     /translation="MRVVDSLFAVLALPWLPWLLATLIVLLAFVLWQGFVLRLAPLRR
                     ALARASELLDRGEGTVAFRQRFPSVFEAIAQDPVLGEAWRSYASTLTPAPGNEQTLGS
                     ARAPHSVFDDTLFARVGINLRFYQAVPNILVGLGLLFTFLGLVAALHFASGGVTADDI
                     REAQAALRELLAAATFKFVTSIAGLGASLVFSWREKVQLHAVQAEIGRFCRLLEARVV
                     PLTPEALLVDQIRETQQIGRLVARMGRSLIIRAPEGIEERLADELTLATRPLKAALER
                     VARRFETLDERVASELVSAGRSLGDLATHAATSGAGTPEPPPDTGSGMDRQISTPPPA
                     RAPDERSPASARGSPSPAMRQLQHRLDQAAVAVQDGLDRLRGRPPLDRAAIETVLLTT
                     HDRVRDARHTLNSVAEAILGEIAEPAAAAAAHELLGDIDAGLKEARQALQEVFEGVST
                     GRRADG"
     gene            9395..11569
                     /locus_tag="P5558_09970"
     CDS             9395..11569
                     /locus_tag="P5558_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY24678.1"
                     /translation="MAKVVVIDDRVTNRNILTRLALSVEEGLQVQSFASPIEALTRLQ
                     ATNPPDLIITDYNMPEMDGATFIETLREQRAFADVPIVVVTVYEDRDFCYRALDAGAT
                     DFLLSPVDHLEFRARARNLLTMRRQQKMLARRAADLEQALHEQQGPAFDNPAARLQAF
                     LDRVPLAISAIDRFGRLAMVNTTHEELFAARRDDLIGKTLIEAFGEAYATRHTVLDEK
                     VLESGQPLGSPRQEVLVTGGRERDLVSVKAPVLDEAGEVDGVITFALDVSELRLDRNT
                     TDLAVRTDQLTGLISASAFEERAEQELARARRQNEMLAILVLDIDRFKTINEAFGNSF
                     GDRLMRAVAGRLAGRLRETDCIARLRSDEFAILQPALKRPDDSAELARRLGDAFAEPF
                     VIEKEEVHVSASVGITLFPADGKSVSALLKNADLAMYRAKASGRDAYRFFAAEMNLAA
                     RRAVTLERELRQALAGEQFLVYYQPQIELASGRIVGMEALVRWNHPHRGIVRPGEFIG
                     LAEDIGLIAPLTAWVLRTACLQHRTWCDKGLLGMQLSVNLSPVQFRERGVELMIERIL
                     KETGVTPSMLDIELTENAVIENSQTATASLKYLNELGVTLSIDDFGTGYSSLSYVKRL
                     PVQRLKIDQSFVQNLEKGSSDEVIVRAIVNLGHSLGLRVIGEGVETEGQHRRLRRLGC
                     DEVQGDFICPALSVAEFERRLALRQLNAAIDGEAADSLAGID"
     gene            11707..14328
                     /locus_tag="P5558_09975"
     CDS             11707..14328
                     /locus_tag="P5558_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY24679.1"
                     /translation="MTLADELLKAWSRLHRRLAGRADSEHEQIVIRIVITTAMFLYII
                     SVDHGAEDAARIVRDSALIYAFAATASVGLLVHLLARPRRSPARRFCGIVTDAIGVNG
                     AMFAGGEAAMLFFPLLLWSILGHGFRYGRLYLAVSAVLSTVLFIGVVVLSPAWRGLGV
                     LDIGLVMSLVMLPGYFAVLLGKLEGAVHKAEDASRAKSRFLATMSHEFRTPLNAVIGM
                     TDLLRTTPLDAEQRDMVATTRSAAHSLLALVNDVLDIAKIEAGRFVIDEEPFDLHRQL
                     ATLRRMLYHEAQAKGLYLRLRLDPRIPPALIGGRGPLHQILVNLTANAIKFTNEGGVL
                     LDVRSLGSEPDRPRLRFEVHDTGIGIAVEAQQRIFESFGQAEGDTPRRFGGTGLGLAI
                     AKELTELMQGTIGVVSAPGQGSSFWVELALRRDPANSEAEDFGSSGGLSRRAEVVVLG
                     GRAATERAMARLQRLAITAIAEPDIAAAVDQAASSSNPTAILVVDADPAVDVETLVEA
                     LDQRRPNEPVDIVTLLERRREHLAASLADLAEADDDSGLERALRAALVGDGQLHGAIG
                     SESLRANTVSNVLLVEDNRTNQHVIGKILEHAGHAVTLADSGEECIELMEANAFDVVL
                     LDLNMPGMSGFNTLKMLRFMLPIPDLPPVVALTADATAETRDAAMALGFAAYLTKPID
                     TLQMLATIDRLAGQQVRHRARALPPSAATVLLLPEAAERATLARAAGRSPIEVAPELS
                     PPAATTARSGGLDTRKIDTLLELDAGDGFFAQVVDDYLLDVTQLEKELHEAVAQGNAR
                     AFRDAAHALKSSSAHIGAQAVFEFCVGLRHLDDHALLMRASGDLELLRQEIERARAGL
                     LQCKERALRAPSRREIG"
     gene            complement(14513..15469)
                     /locus_tag="P5558_09980"
     CDS             complement(14513..15469)
                     /locus_tag="P5558_09980"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRY24680.1"
                     /translation="MALSHQFVRGLQDAVNDNFDLLPSELRRMAAPVVPLRRPSLEVD
                     LPTLATKLEPETGILWARMKHQDRACYTPSLVGDSRSYQIYLQEQCGHLSADEMPFRY
                     LVWTSEAKGAFSLGGDLAFFTECVRSGNERALRDYAQRCIDVLHDNYNALGLPVLTVA
                     LVTGDAIGGGLESMITNDIVVAERGAKFGLPEILFNLFPGMGGYSYLVRKVGERAARA
                     FIEDGKSRSAEEMLELGLVDVLAEPGQGEAALREHLGSHGSRFNMLRTIRRACKRVDP
                     VQKTELLDIVNIWVDLAMQLGADDLRRMDCLARVQEKKRVRT"
     gene            complement(15726..16262)
                     /locus_tag="P5558_09985"
     CDS             complement(15726..16262)
                     /locus_tag="P5558_09985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY24681.1"
                     /translation="MVTGQAVLAPELQLVPAWATFVTSMFLHGGLVHLASNLLFLWVF
                     ANNVEDRLGHFRFAFLYLAAGLAAAGLQILPDPASTVPMIGASGALSGVLGAYFMLFP
                     RARVVVFFPFSFMLLHEMRAAWLLAIWFGFQLLSAFTAPAGAGVAWWAHVGGFVAGAV
                     LVMPLSHRRLLRRRGPWG"
     gene            complement(16427..17032)
                     /locus_tag="P5558_09990"
     CDS             complement(16427..17032)
                     /locus_tag="P5558_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HRY24682.1"
                     /translation="MGRIYEYAGIRPVIDASAFVHPEAVVIGDVIIGAGVYVAPLASL
                     RGDLGRLIIEAGANVQDCCVMHGFPGKDCVIEEDGHVGHGAILHGCRIGRNALVGMNA
                     TVMDGAVVGANAFVAAMAFVKAGFEVPEGHLVAGVPARVVRPLEPSEIAWKGKGTREY
                     QELARMSADTLRPCEPRRAVEPDRPRCPSLTAIRPKLESRS"
     gene            complement(17135..17419)
                     /locus_tag="P5558_09995"
     CDS             complement(17135..17419)
                     /locus_tag="P5558_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24683.1"
                     /translation="MGATIDYQKALDDPAAHFSDPEAVRSAPGLTTEQKVAILRQWEY
                     DQSELAVAQEEGMPGNGAPLLQRIAETLMELDPVGDDRPAPTKHRVPPSS"
     gene            complement(17525..17776)
                     /locus_tag="P5558_10000"
     CDS             complement(17525..17776)
                     /locus_tag="P5558_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRY24684.1"
                     /translation="MNDVPPRATAVLVDARGLNCPLPLLEARRALALLPGGATIVVLA
                     TDPAAPDDFIEFTEATGHELADVVEQDGLFRLTLIKRPA"
     gene            complement(17823..18488)
                     /locus_tag="P5558_10005"
     CDS             complement(17823..18488)
                     /locus_tag="P5558_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY24685.1"
                     /translation="MELVPLDDYRRLPEAEMVARAESFAAELAHRRTVRDFADEPVPE
                     AVVLAAIRAATTAPSGANQQPWHFCLIGKGELRAELRAAAEAEERAFYAERAGPEWLE
                     ALAPLGTDWRKPFLETAPWLIAVFAERHGIAADGARRKHYYVGESVGIATGLLLATLH
                     RAGLVTLTHTPSPMGFLNRVCGRPEREKPFILIVTGYPAADARTPAAARVRKPLDQVL
                     SRR"
     gene            complement(18534..>18854)
                     /locus_tag="P5558_10010"
     CDS             complement(18534..>18854)
                     /locus_tag="P5558_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY24686.1"
                     /translation="LTPVRLDTTHPDAWHAPTPGAEVLPLHRHRDEVVRLERLAPGTV
                     VDREVGPGGIEAFLVDGRIEKVGRLHRAWAWFRQPAGSQFSLRALDASLIYIKEGHLP
                     AGRP"
BASE COUNT         3072 a         6516 c         6374 g         2892 t
ORIGIN      
        1 tcatcgacca cacgatcaag tcagcaaggt ggggtaggcc ggacgctcac gatcatacgc
       61 gacattcggg cttgggttgt cgcgtattcc gcgacagcgc ccgccggaat gtcgcagaac
      121 ccgcgacata acgattcgtg atggcgcgtc tgacgcgaca tgcgcccgga aacgtcgcgg
      181 attctgcaac acaaggccac gaacgaaccc acggaatcga cgatcgaacc cggcgaatca
      241 acgaatgaac ccgcggaatc gacgaacgaa cccagcccgc gaccaacgaa cccgagggcc
      301 tataacctgc tgtaacataa acacttattt cctctcccgg tgaatgctgc gccttcgccg
      361 gaccttcgaa ctcggaaaat gccgaaccaa cccggccgac ctcgccgagc gcttgcgtct
      421 ggcgccggaa tgggctataa atcctaacgt ggatcacgag cggcggtgcg ctcccgtcgg
      481 ctggccggct gccgacggcg atggcggggg actgcgttgg aagggctcga ccgaaagccg
      541 tcatgcgggc attctccgcc cgggccggac ggttcgcctt tacccgaccg cgcaagccct
      601 cctcgaccgc gcaagaaagt gaccgccaat gccgaacgcc gccacaccgc ccgagctcgt
      661 cctcttcgat ctcggcggcg tgctgatcga ctgggacccg cggcggctct accgcaagct
      721 cttcgccgac gaggcggcga tggagcagtt tctctcgacc gtctgcacgc ccgcctggaa
      781 tctcgagctc gaccgcggcc ggcccttcgc cgtggcggtc gaggagctgg ccgccctcta
      841 tcccgaggag cggccgctga tcgaggccta ccggcagcgc tggctcgaga tgatcggcgg
      901 ccccatcggc gagaccgtga ccctgctgga ggagctggcg gcgcgccgga caccgctgcg
      961 cgccctcacc aactggtcgg ccgagacctt caccctcgtc cacccgcatc cggactatgc
     1021 gtttctcgaa cggttcgccc agatctacgt ctcgggggag ctccgcatga tcaagccgaa
     1081 tgacgacatc ttcgagcacg tcctcgagga caccggcctc gctgccgaat cgatcctttt
     1141 catcgacgat tcggccgcga acatccgcac cgccgagcgg ctcggcttcg tcacccatcg
     1201 ctttgccggt gccgacgggc tcgagcttca cctgcgcgac ctccagctca tctgagccgc
     1261 atgggccgcc tgctcgccac gctcgcggcc gtcggcttcg gcctcgggct gggcgcggcg
     1321 gctctggcag gcttcctcgt ctgggcctcg ctgccgatgc tggaaggcga gatccgcctc
     1381 gccgggctgg gtgccccggc ccgggtcagc cgggacgagc tcggcgtgcc gctgatcgag
     1441 gcggcgagcg agcgcgacgc ggccatggcg ctgggcttcg tccatgccca ggaccggctc
     1501 tggcagatgg agatcaaccg ccggatcggg gccgggcggc tcgccgaggt ggtgggcgaa
     1561 cgcggcctgg cggtcgaccg cttcatgcgc accctcggcc tctaccgcgc cgccgaggcg
     1621 agcctcgacc atctctccgc cgaggcgctg gaccggctcg ccgcctatgc cgagggcgtc
     1681 aacggctttc tcgcccagcg tgccggcccg ctgccgcccg agttcctgct cctgcgccat
     1741 cggccggagc cctggcggcc ggccgacagc ctcgtcctga tcaagctgat ggcgctcgat
     1801 ctcgtgtcga gctggcggag cgagctgacc cgggccagcc tgctgggccg gctcccgccc
     1861 gccgccctcg ccgatctctg gccgccgccc cggccggacg aggtgacgac gctgccggga
     1921 gcggcgcagt gggccggtgg actgccgtcc ggggcaggat cgaccgaccc ggctgccgcc
     1981 atcgagacgg ccgcccttgc cgaagcgctc ggcgcggcgc tgccgctcgc cgacaatgcc
     2041 gggctcggct ccaacatctg ggccgtggcc ggcgcccata cggccagcgg cgcagcgctg
     2101 ctcgccaacg acccgcatct cggcctcgcc accccggcgc actggtacct cgcggccatg
     2161 cgcacgcccg ccggcaccgt cttgggcgcc accctgccag cactgccggc cgtggtcatc
     2221 ggccgcaacg agcacatcgc ctggggcttc accaacaccg ccggcgacac cgacgacctc
     2281 ttcgtcgagc ggctcgatcc ggccgatccg gaccgctacc tgacgccgga cggcagccgg
     2341 ccgttcctcc gccgcgacga ggtgatcgag gttcggggcg gcgaggcggt gcacctcctg
     2401 gtgcgcgaga cccgccacgg tccggtggtc tcggacatca tgacccgggc cgccgaggtc
     2461 gccggcgagg ggcgggtcct ggccctcgcc tggacggcgc tcctgcccga ggaccgcacc
     2521 atcgaagccg gcatcgggct cgcccgggcc agggactggg acggtttcac ccgggccatc
     2581 gcgctcttcc acagccccac ccagaacgcc ttctatgccg atgcggaggg gcggatcggc
     2641 atggtcatgg ccggccggct gccgctccgc cgcgccggcg acggcagcct gccggcgccg
     2701 ggctgggacg gcagccacga ctggctgggc ctgctgccgg cggcggagaa cccggcaacg
     2761 cccggggcca ccgcggcggg gttcctgcag aatgccaaca accgcctggt cgacgaccgc
     2821 tatccccatc tcgtcaccgc cgactggaac gacgatctca gggcccggcg catcaccgcc
     2881 gtgctcgacg cggccaccgc cgagggcgcg ccgctcgaca tcgacgccat gcgccggctg
     2941 cagaacgacc ggctctcgac cctggccgcc gatttccggc cccggctcga agcggtggcg
     3001 ccggccgacg ccgagacggc cctgatactc gctgccatcg cggcatggga cggggcggcc
     3061 atgccggatc ggccggagcc gctggtcttc caggcctggt atcgatcact ggtccgccat
     3121 gtcctggcgg acgagctcgg cgagggtttc gccgcctttc ggggcatccg gtccgaggcc
     3181 atgcggcaca tcctggagca ggcgcccggc tggtgcgacg atgtcgccac ggccgaccgg
     3241 accgagagct gcggtgacat ggcaactgcc gcactggcga gttccctcga cgagctgcgg
     3301 gcgacctatg gcgcagactg gcgaagctgg cgctggggcg aggccagccg ggcgcgcctc
     3361 gcacatcgcc cgctggacgc ggcaccgggc atcggccggc tcttctcgat cgtcaccggc
     3421 agcggcggcg gcgacgccgg caccgtccat gtggcccgct atgccgccga catgccctat
     3481 gcgaccgtcg cggcggcgag cctgcgcctg gtcgccgatc tcgcccggtc gggaacgctc
     3541 catgccatcc tgccgacggg tcagtccggc cacccgctgt cgccccattt cagcgaccag
     3601 acaggtttgt ggcgcaacgg tgagttgcac acgctggaaa tcgcgcagcc ggtaggccga
     3661 ggccgctccg aactggtgct caggccatga aatcgccacg aagggctacc gcaaattcct
     3721 gttatcacct atgagactgg ctctccatac cccagatgcg agaaaccgat atgcagttga
     3781 gcgagcttcg cagcttcggg caacgtctcc gctcactgcg cgtcgggctg gaattgtccc
     3841 aggccgaact cgcgcgccgt atcggccggc atcagacggc tatcgggccc tatgagcgcg
     3901 acgagtacat gccgggacgc gacgtcgtcg agaagctcgc ggccgcgctg gaaacgagcc
     3961 cggaatacct gcagttcggg cggatgccgt accgcagcag cctgccgcag ctcggccggc
     4021 tcggtgcttc gggacagctg acgagccccg cccatgcggg cccgctggcg ctggccgacg
     4081 atcgcctggc ggccctgacg ctcgaggacg aggcgatgcg gccggtcttc cgcgccggcc
     4141 agaccgtgct ggtcgcccgg ctggcgacaa ccgccctcga gccgctcatc ggccggcatg
     4201 tgctcgccga gctcgacgac ggccgccagt tcctgcgccg cctcgcacct gctgccgaca
     4261 gcggaaacta cgttctggac gcgtataatg cggcaccatt ggggcccttg cggccggttg
     4321 cggcacggtt ggtcctgggc catctggagg ttgaggccct gtcgtttcgc gcccaagagc
     4381 caggcgcatc aggttgatga gaaattcgcc ttttatctgg aagtttaaaa tatcgcaggt
     4441 attttagcta aatcaggttc agttggcgag cgactcgggg agcacacaac cccagcgtgc
     4501 gccgcagcag gagtcgtctc gtcatgaacc aggtcgccag caagcccgtc gggaagggtg
     4561 tcggagggcg cagtcgcgcc ctggacgtcg atcactatgt cagcctccgc atccggcagc
     4621 gccgcatcat gctcggtctc acgcagcagc agatggccga gctgatcggg gtcacctacc
     4681 agcaggcgca caagtacgag accggcatca accggatctc ggccggccgg ctctatcaga
     4741 tcgcccaggc cctgggcgtc gacatcggct atttcttcga cgacctcgac cccgacaagc
     4801 agagccggcc gaaatcgacg gagatgatgc cgcagcagcg catgctgctc gagctcgccc
     4861 gcaacttcgc cgcgatcggc aaccgcaagc atcaggacgc gatctgcaac ctggcgcgtg
     4921 tgctggcctc gggcgagcgc gccgaaagct gaccggtcta ccgcttttgg ggggcggcga
     4981 cggggcgctc aggcgcggcg cgttgcggcg agccggatga tccggatcgt ttcggcccag
     5041 aggcccaggc cgtggagctc tccggcgtgg aaccagccga cggcggcggc atcgcccccc
     5101 gctatcgccg tgccgggcgc agcctcggcg gtaaagtcga tcagggtgta gtggtagagg
     5161 acccgggccc catcgggtgt gtgctccacc agatcgacga cggtcaggag gtcgaggctg
     5221 gcgaggtcga gcccggtctc ctcgctgacc tcgcgtcgcg cagtctcggc caccgtctcg
     5281 ccgagctcct gagcaccacc gggcagcgac cactggccgg cccgcggcgg cttgccacgg
     5341 cggatcagca gaacccgatc gtcccgccag accacgacac cgacgccgat gatcggccgg
     5401 tccgggaagt cgcgccgcat tgccctctcc ctgggggtgc cgattgccgc cgtcagccga
     5461 tcagcgccag tgccgcagca tagcgtgtga gcttcgtgcc ggcgacctcg gcggcactgc
     5521 gcatgccgat caccggcacg ggcccgagca cgcgcccgag aacggtcgcc tcgtcgacgc
     5581 cgggaccgaa gaagccgatg cctcgctcga tcgagctgta gaagagcgct gccctgggcg
     5641 gtcggccgcc cagcttctgc aggagatccg cagccagcgc ctggagatgc tcgagcgcca
     5701 gtgccggatc ccggcggaag agctggatcc ggctgggcag gcctccacca ccgagcccga
     5761 tgcccgagat ctcgatcgag ccgcgcagcg gatcggcgtg gacgataccg aagagggcat
     5821 ccgccggcgc gagcgacgaa ccggcgagcc ccacctccgt cgggacctgg gcgagcacca
     5881 gctggcgacg aagctgccgc tggtcgcgaa gcacgaggtc ccccgcttcc tcggcgagcc
     5941 aggccgtcgc cgcccggccg tccagtgccg agagcgtgct gccggcaccc gccgtgaccc
     6001 gatgcgccgg accgagcggt cggcagccat gagtgatgcc agccaccatc gtgacattgc
     6061 cggaaagcag cacgccggtc gccgcaccag ccgacggcga accggcaatc tgcgcgtgat
     6121 cgaagctgga tgacccttga gccccggtca gcacggccga ggtgcgcgcg gcgagcgccg
     6181 cgaccgccgc tcccgcatcc gcggcatccg gcacatgcag gatgccacag gtcgcttcct
     6241 cggccccggt cggcggtgaa ccggcgaagg ggtggagggt gacgccttcc agcggcaacg
     6301 gcagcgcggc gatggcaccc tcgccaatca gctcgcgacc accagcgaac accgcggtgc
     6361 tggccgcccc gagccaggtc tggatgccgg tccgctgacg cagcccgtcg agcatgatat
     6421 cggccatctc ggtcgtcggg tcggcgagga agacgaggcc caggggcgct ggtgccgcgt
     6481 cgccgtcgag ctgatccagg acgctgcgca ggagttgcgg ccaagggcga ccagccgcgt
     6541 agacgagacc gttcatgccg cagtcaggcg gcggagcaca cacggcggcg ttgaactgaa
     6601 ttcaaccatc aaggaaatat cttgaccatg tttcaacttt ttggcaatac ccggaagcgt
     6661 tcagtcatcg gttgagcgaa actgccggtg accgacgtgc tcgattgacg agatgggacc
     6721 ctggggggac tgttcgcaag gacagtccct tcccgtcgtc gattcgccag cgcctgggcc
     6781 atagggacct agaccatagg cgcgtctccg gccatgccgt cggcgacatg cgcctcatcg
     6841 gggtacgaag ctggtctcga gcgtctccag aagctcggcc agggccgccg catccggtcc
     6901 gcgactgaga aagcgtacat cgatgcgtct gttcacccgc cgtgcctcat cggtggcgcc
     6961 gtccgcgacg ggccggcggg cctcgtagcc gctcaccccg agcagggccg cggcgctgcc
     7021 gttgcgcagg tcgtcgaggc gcggctcggc cgcgatcatg gcccggtagg tcgagcggct
     7081 gcgggccgcg gacaaggccc agttgtcggg aaagggcccg ctgcggatcg gccgatcgtc
     7141 ggtgtggccc tcgatcagca cggtctccag cacatcgtgc acgccggtcg gacagccctc
     7201 ggcctcggcc atcgcggcgt agcacggcag atagcgccac aagacctcgg cgagcacgcc
     7261 gatcgccgct cgccccgtct cgctcagctc ggcctcgttc gtggcgaaga gcagcgattc
     7321 cggcaggcgc aggatgccgt tctcgacatc gacctcgacc tcgaggccgc gcgcggcgag
     7381 cgcctgtcga atgagggtca ggagctcggc ccgcagcgcg tcacgcgcga gcagttgccc
     7441 aatgatgccg gcgaggcgat cgcggtcggc gagcaggcga tcacgctcga gcgtcagggc
     7501 ggcctgatcg ctctcgagcg cgtcgcgctc cagctccagc acgttgcgtt ccttggtcag
     7561 cgcgtcgagc gtctcctccg ccgcgtcctc ggcactgcgg aagttgagcg cgaaggccat
     7621 gagaatgatg atgaacagga agagcagacc gaccatcagg tcgctcacag agacgaagta
     7681 gctttcgtcc tcggcgtgcc ggccgccgcc cttcgccgca ccgccccagc cgcgtgccca
     7741 gccggccccg ttcacggctc agccatccgc ccgtcggccg gtgctcaccc cttcgaacac
     7801 ctcctgcagc gcctggcgcg cctccttgag gccggcgtcg atgtcgccga ggagctcatg
     7861 ggccgcggcc gcggcggccg gctcggcgat ctcgccgaga atggcctcgg ccacgctgtt
     7921 cagcgtatgg cgggcgtcgc gcacgcgatc gtgtgtcgtc aggaggaccg tctcgattgc
     7981 cgcccggtcg aggggtggcc ggccacgcag ccggtcgagg ccgtcctgca ccgcgacggc
     8041 cgcctggtcg agacgatgct gcagctggcg catcgccggc gacggcgatc cgcgtgcgga
     8101 tgcaggggac ctttcgtcgg gcgctcgagc gggcggcggg gtcgaaatct gtcgatccat
     8161 gcccgagccc gtgtcggggg gcggctccgg cgtcccggca ccactggtcg ccgcgtgcgt
     8221 ggcgaggtcg ccaagggaac gaccggcgga caccagctcg ctggccacgc gttcgtccag
     8281 tgtctcgaag cgccgcgcca cgcgttccag cgccgcctta agcggtcggg tcgcgagggt
     8341 cagctcgtcc gccagccgct cctcgatgcc ctccggtgcg cggatgatca gcgaccggcc
     8401 catgcgggcg acgaggcggc cgatctgctg cgtctcgcgg atctgatcga cgagcagcgc
     8461 ctccggggtc agcggcacga cacgcgcctc gagcagtcga cagaaccggc cgatctcggc
     8521 ctggaccgcg tgcagttgga ccttctcgcg ccaggagaag accagcgacg cgccgagccc
     8581 ggcgatcgac gtcacgaact tgaaggtagc ggccgccagc agctcccgca gcgccgcctg
     8641 cgcctcgcgg atgtcgtcgg cggtcacgcc gccgctggcg aaatggagcg ctgcgacgag
     8701 gccgagaaag gtgaagagca gcccgaggcc gacgaggatg ttcggcactg cctgatagaa
     8761 ccggaggttg atgccgaccc gcgcgaagag cgtgtcgtcg aacacgctgt gcggtgcgcg
     8821 ggccgagccg agcgtctgct cgttgccggg cgccggggtg agggtggacg cataggagcg
     8881 ccaggcctcg ccgagcacgg gatcctgggc tatcgcctcg aacaccgagg gaaagcgttg
     8941 tctgaacgcg accgtcccct cgccgcgatc gagcagctcg ctcgcccgag cgagcgcccg
     9001 acgcagggga gccaggcgca gcacgaaacc ctgccaaagc acgaaggcca ggagcacgat
     9061 cagggtagca agcagccagg gcaaccaggg cagcgccaga accgcgaata ggctgtcgac
     9121 gaccctcatg ccggccccct cgtaccgctt gcaagaccgg gaacgcggag ccggttgtgg
     9181 tcggctcgac tagactgcat gctcgtcgcc cgaccgatga cggcggtgat cgaacaggaa
     9241 acccgcagaa gttgattgat cttgatgtgt gttcacctaa aacttgctgc gctaaccgac
     9301 cttgcctagt ctggccgtgg caaggattgg tcttcggatc gggcggatcc ggcatggtcc
     9361 ccggcgacct atgataagcg acaagtgaca gtccatggca aaagttgtgg tcatcgacga
     9421 ccgggttacc aaccgcaata ttctcacgcg gctcgccctc tcggtcgagg aaggtctgca
     9481 ggtgcagtcg ttcgccagcc cgatcgaggc gctgacgagg ctgcaggcca ccaatccgcc
     9541 ggacctgatc atcaccgact acaacatgcc ggagatggac ggcgccacat tcatcgagac
     9601 gctccgcgag caacgtgcct tcgcggatgt accgatcgtg gtcgtcaccg tctacgagga
     9661 tcgcgacttc tgctaccggg cgctcgatgc cggcgccact gatttcctgc tgagccccgt
     9721 cgatcatctc gagtttcggg cccgcgcccg caatcttctg accatgcgcc gtcaacaaaa
     9781 aatgctggca aggcgcgcgg ccgatctcga gcaggcgctg cacgagcagc agggtccggc
     9841 cttcgacaat ccggccgccc gcctgcaggc cttcctcgat cgggtcccac tggcgatctc
     9901 cgcgatcgac cggttcggcc gcctcgccat ggtcaatacc acgcacgagg agctgtttgc
     9961 ggcccggcgt gatgatctga tcggcaagac cctgatcgag gctttcggcg aagcctacgc
    10021 cacccgtcat accgtgctcg acgagaaggt cctcgagagc ggccagccgc tcggctcgcc
    10081 gcgccaggag gtgctggtca ccggcggtcg cgagcgcgat ctggtcagcg tcaaggcgcc
    10141 cgtgctcgac gaggccggcg aggtggacgg cgtgatcacc ttcgccctcg acgtcagcga
    10201 gctgcggctc gatcggaaca ccacagatct cgctgtccgc acggaccagc tcaccgggct
    10261 gatctcggcc tcggccttcg aggaacgtgc cgagcaggag ctggcccggg cgcggcgcca
    10321 gaacgagatg ctggccatcc tcgtcctcga catcgatcgc ttcaagacca tcaacgaggc
    10381 cttcggcaac agcttcggcg accgcctgat gcgcgccgtc gcgggccggc tcgcaggccg
    10441 cctgcgcgag accgattgca ttgcccggct ccgctccgac gagttcgcga tcctgcagcc
    10501 ggccctcaag cgcccggacg actcggcgga gctcgcccgc cgcctggggg atgccttcgc
    10561 cgaacccttc gtcatcgaga aagaggaggt gcatgtcagc gccagcgtcg gcatcacgct
    10621 ctttccggcc gacggcaaat cggtcagcgc gttgctcaag aacgccgatc tcgcgatgta
    10681 ccgggcaaag gcctcgggcc gcgatgccta ccggttcttc gcggccgaga tgaacctcgc
    10741 ggcgcgccgc gcggtcacgc tggagcgcga gctccgccag gcgctcgccg gcgagcagtt
    10801 cctcgtctac taccagccgc agatcgagct cgccagcggt cgaatcgtcg gcatggaagc
    10861 gctggtccgc tggaatcacc cgcatcgcgg catcgtccgg ccgggcgagt tcatcggtct
    10921 cgccgaggat atcgggctga ttgcgccgct gaccgcctgg gtgctgagga ccgcctgcct
    10981 gcagcatcgc acctggtgcg acaagggcct gctcggcatg cagctctccg tcaatctgtc
    11041 cccggttcag ttccgcgagc gcggggtcga gctgatgatc gagcggatcc tcaaggagac
    11101 cggggtgacg ccatcgatgc tcgatatcga gctcaccgag aacgccgtga tcgagaacag
    11161 ccagaccgcc actgcctcgc tcaagtatct caacgagctc ggggtgacgc tctcgatcga
    11221 tgatttcggc acgggctact cgtcgctctc ctacgtcaag cggctgccgg tgcagcgcct
    11281 gaagatcgat cagagcttcg tccagaacct cgagaagggc tcatcggacg aggtcatcgt
    11341 ccgagccatt gtcaatctcg gccacagcct gggcctcagg gtcatcggcg agggcgtgga
    11401 gacggaaggt cagcaccgcc ggttgcggcg cctcggctgc gacgaggtgc agggcgactt
    11461 catctgcccg gcgttgagcg tcgccgagtt cgagcgccgt ttggcgctgc gccagctcaa
    11521 tgccgcgatc gacggcgagg ccgccgattc gctggccggc atcgattgac gaggtgcggc
    11581 gtcgaggtga tcgtggcggt ggacagcgcg atccggatcg agtgttcctc gacgccacgc
    11641 gatcgaccgt agtcgcggcg acggtcgcgc cggagtgacc gtggatctgc tgcgaatgag
    11701 cgaggcatga ctctggccga cgagttgctc aaagcctgga gccggctgca tcgccggctc
    11761 gctggccgag ccgacagcga gcacgagcag atcgtgatcc gcatcgtcat caccacggcc
    11821 atgttcctct atatcatttc cgtcgatcac ggggccgagg acgccgcccg catcgttcgc
    11881 gacagtgcct tgatctacgc cttcgcggcg acggcctccg tgggcctgct cgtccacctg
    11941 ctggcccgcc cgcgccgcag cccggcacga cgcttctgcg gcatcgtcac cgatgccatc
    12001 ggcgtcaacg gtgccatgtt cgccggtggt gaggcggcca tgctgttctt cccgctgctg
    12061 ctctggtcga ttctcgggca cggcttccgc tacggccggc tctatctagc tgtctccgcg
    12121 gtgctgagca cggtgctgtt catcggcgtg gtcgttctgt cgcctgcctg gcgcggcctc
    12181 ggcgtgctcg atatcggcct tgtcatgtcg ctcgtcatgc tcccgggcta tttcgcggtc
    12241 ctgctcggca agctcgaggg agcggtgcac aaggccgagg atgcgagccg ggcgaagagc
    12301 cgatttctcg ccacgatgag ccacgagttc cgcacgccgc tgaacgcggt catcggcatg
    12361 acggacctcc tgcgaacgac accgctcgac gccgagcagc gcgacatggt ggcaacaacg
    12421 cgaagcgccg cccacagcct cctggcgctg gtcaacgatg tgctcgacat cgccaagatc
    12481 gaggcggggc gcttcgtcat cgacgaggag ccgttcgact tgcatcggca gctcgcgaca
    12541 ttgcgtcgga tgctttatca tgaggcacag gccaaggggc tctatctccg acttcggctc
    12601 gatccgcgaa ttccgccggc gctgatcggt ggccgcggcc cgctgcacca gattctcgtc
    12661 aatctcaccg ccaatgcgat caagttcacg aacgagggcg gcgtgctgct cgatgtccgc
    12721 tcgctcggca gcgagccgga tcgcccgcgc ctgcgcttcg aggttcacga caccggcatc
    12781 gggatcgccg tggaggcgca gcagaggatc ttcgagagct tcggtcaggc cgagggagac
    12841 acgccccggc gcttcggcgg aaccgggctc ggccttgcca ttgccaagga gctgacggag
    12901 ctcatgcagg gcacgatcgg cgtcgtcagc gcgcccggcc agggatcgag cttttgggtc
    12961 gagctggcgc tgcgtcgcga cccggcgaat agtgaggcag aggacttcgg atcgagcggt
    13021 ggcctcagcc gccgggcgga ggtcgtggtc ctgggcggcc gagcggcaac cgagcgggcc
    13081 atggcaaggc tgcagcgact ggccatcacg gccatcgccg agccggacat tgcagccgcg
    13141 gtcgatcagg ccgcgtcgag cagcaatccg accgccatcc tcgtggtcga cgccgatccg
    13201 gccgtcgacg tcgagacgct ggtcgaagcg ctcgaccagc gtcgcccgaa cgagccggtc
    13261 gatatcgtga cgctgctcga gcggcggcgc gagcatctcg ctgccagcct cgccgatctc
    13321 gccgaggcgg acgacgacag cgggctcgaa cgggcgctgc gcgccgccct tgtcggtgac
    13381 gggcaactcc atggtgcaat cggcagcgaa agtcttcggg cgaataccgt ctcgaacgtg
    13441 ctgctggtcg aggacaatcg gaccaaccag catgtgatcg gcaagatcct cgagcatgcc
    13501 ggccatgcgg tcaccttggc cgacagcggc gaggaatgca tcgagctcat ggaggcgaac
    13561 gccttcgacg tcgttctgct cgaccttaac atgcccggca tgagcggctt caacacgctg
    13621 aagatgctgc gcttcatgct gccgatcccg gacctgccac cggtcgtggc gctaacggca
    13681 gatgccacgg ccgagacgcg cgatgcggcg atggcgctcg gctttgcggc ctaccttacc
    13741 aagccgatcg acacgctgca gatgctcgcg accatcgacc ggctggccgg tcaacaagtg
    13801 cggcacaggg ctcgtgccct gccgccctcg gctgcaacgg tcctgctgct gcccgaggcg
    13861 gccgagcggg caaccctggc gcgggctgcc ggacgatcgc ccatcgaggt ggcgccggag
    13921 ctgtcaccac cggctgcgac gacggcaagg tcgggcgggc tcgacacgcg caagatcgat
    13981 acgctgctgg agctcgatgc cggcgatggc ttctttgcgc aggtggtcga tgactacctg
    14041 ctcgatgtga cgcagctgga gaaggagctg cacgaggccg tggctcaggg caatgcacgg
    14101 gcgttccggg atgctgcgca tgccctgaag agcagctcgg cgcatatcgg cgcgcaggcg
    14161 gtgttcgagt tctgtgtcgg tctgcggcac ctcgacgacc atgcgctcct gatgcgtgca
    14221 agcggcgacc tcgagctgct gcgccaagaa atcgagcggg cccgagcagg gctgctgcaa
    14281 tgtaaagagc gagccctgcg agcgccgtcg cgtcgagaaa tcggctagcg cccggcagca
    14341 cggtgttcac cagatgcccg acggcgagct cgagccccag gcagagcagg cgttccgggg
    14401 cgatggtgaa accctgagcc gacatgaccg agctccgtgg ttggtgaggc gaggggcgct
    14461 tgccccggcc cgggaagcgg gtggcgctag accttggaag ggatgccgct cctcaagtcc
    14521 tgacccgctt cttctcctgc acgcgcgcca ggcaatccat gcgccggagg tcgtcggcgc
    14581 cgagctgcat ggccaggtcg acccagatgt tgacgatgtc gaggagctcc gtcttctgca
    14641 cggggtcgac ccgcttgcag gcgcgccgaa tggtgcgcag catgttgaag cggctgccgt
    14701 ggctgccgag atgctcgcgc agcgcggctt cgccctgacc cggctcggcc agcacatcga
    14761 cgaggccgag ctcgagcatc tcctcggcac tcctggactt gccgtcctcg atgaacgcgc
    14821 gtgccgccct ctcgccgacc ttgcgcacga ggtaggagta cccgcccatg cccgggaaca
    14881 ggttgaagag gatctccggc aggccgaact tcgcgccgcg ctcggccacc acgatatcgt
    14941 tggtgatcat cgactcgagg ccgccgccga tcgcgtcgcc cgtgaccagc gccacggtga
    15001 ggaccggcag gcccagcgcg ttgtaattgt cgtgcagcac gtcgatgcag cgctgcgcat
    15061 agtcgcgcag cgcccgctcg ttgccgctgc gcacgcactc ggtgaagaag gcgagatcgc
    15121 caccgaggct gaaggcaccc ttggcctcag aggtccagac gagataacgg aacggcatct
    15181 cgtcggcgct cagatgcccg cactgctcct gcagatagat ctggtagctg cgcgaatcgc
    15241 cgaccagcga cggcgtgtag caggcccggt cctgatgctt catgcgcgcc cagagaatgc
    15301 cggtctcggg ctccagcttc gtcgccagcg tcggcaggtc gacctcgagc gacggacggc
    15361 gcagcggcac cacgggtgct gccatgcggc gcaactcgga gggcaggagg tcgaaattgt
    15421 cgttgaccgc gtcctgcagg ccacgaacga actgatgcga caaagccatt ctctcaccct
    15481 cctggagccg atacttcgcg ctgtacccgc gaaagccccg gcatttgctg ttttgcaact
    15541 gcggatcttg cgtgcggtca gaaatattaa ccttaacgat ccgatgcaag aagtatcgtt
    15601 tcggatttct tgaagttatt ggaaaaacaa ttttattctt ttctgtgatt tttgttttta
    15661 ttagaactac tttttatcaa aaaattgggc aagattttag acgatactgc gtggctcgct
    15721 ggcgctcatc cccaagggcc acgtcgccgc agcagccggc gatggctgag cggcatgacg
    15781 agcacagccc cggcgacgaa gccgccgaca tgcgcccacc aggcgacacc agcgccggct
    15841 ggtgccgtga acgcgctcag gagctgaaag ccgaaccaga tcgccaaaag ccaggcggcc
    15901 cgcatctcgt gcagcagcat gaagctgaag gggaagaaga cgacgacacg cgcccgcggg
    15961 aacagcatga aataggcgcc gagcacgccc gacagggcac ccgaggcgcc gatcatcggc
    16021 acggtcgagg cggggtccgg cagaatctgc agccctgctg cggcgagccc cgcggccaga
    16081 tagagaaagg cgaagcgaaa atgcccgagc cggtcctcga cattgttcgc gaagacccag
    16141 agaaagagca ggttggaggc gagatgcacg aggccgccat gcaggaacat ggaggtgacg
    16201 aaggtcgccc aggcgggcac gagctggagc tcaggcgcca gcacggcttg gccggtgacc
    16261 acggccggga tcagcccgag gctgtagacg gagcggtcca gaccaccatc cctgctgatt
    16321 tgccagaaat aggttgcgac gcacgcgacg atgagcagac aggcgacaac gggcagccgc
    16381 gtcgctcggt tcaggtccct gatcggaaac agcgccgcct gcctcttcag ctgcggcttt
    16441 cgagcttcgg tcgaatagcc gtgaggctgg gacagcgggg ccggtcgggc tcgaccgcgc
    16501 gccgtggctc gcagggcctg agcgtgtccg cggacatgcg cgcgagctcc tggtattccc
    16561 tggtgccctt acccttccag gcgatctcgg atggctcgag cgggcgcacg acgcgcgccg
    16621 gaacgccggc caccagatgc ccctccggca cctcgaaccc ggccttgacg aaggccatgg
    16681 cggcgacgaa ggcattggcg ccgaccaccg cgccgtccat gacggtcgcg ttcatgccga
    16741 cgagcgcgtt ccggccgatc cggcaaccat gtaagatcgc tccgtggccg acatgcccgt
    16801 cctcctcgat gacgcagtcc ttgccgggga agccgtgcat gacgcagcag tcctgcacgt
    16861 tggcgccggc ttcgatgatc agccggccga ggtcgccgcg caatgaggcc agcggcgcca
    16921 cgtagacccc ggcaccgatg atcacgtcgc cgatgacgac ggcctcggga tgcacgaagg
    16981 cgctcgcgtc gatgaccgga cggatgccgg catattcata gatgcggccc acgcctcgtt
    17041 ctcccttgcc gatggccggg ccgcatcagg cggcccgctc ggtgcgagcg caaagcacgg
    17101 cgtgacgcgc gcggcaaccc ggatggccgc cgcgtcagct gctcggcggc acccggtgct
    17161 tggtcggtgc cgggcgatca tcgccgaccg gatcgagctc catcagggtt tcggcgatgc
    17221 gctggagcag cggtgcgcca ttgccgggca tgccctcctc ctgggcaacg gccagctcgc
    17281 tctggtcgta ttcccattgc cggagaatcg cgaccttctg ctcagtcgtg agccccggcg
    17341 ccgacctgac tgcctcggga tcgctgaaat gcgccgccgg atcgtccagc gccttctggt
    17401 agtcgatcgt cgcgcccatt gctcgctcct catcgatacg ctcgccgcac gagctcgctg
    17461 gagatgaagt gccagatctg cagatggttc cccgggcggt cgcttcaatg cgcgcccggc
    17521 cggctcaggc cgggcgcttg atcagcgtca gacggaagag gccgtcctgc tcgaccacgt
    17581 cggccagctc gtgcccggtc gcctcggtga actcgatgaa atcgtcggga gccgccggat
    17641 cggtggcgag caccacgatg gtggcaccgc ccggcaacag ggcgagcgct cgccgtgcct
    17701 cgagcagcgg caggggacag ttgagcccgc gcgcatcgac aaggacagcg gtggctcggg
    17761 gcgggacatc gttcatgccc gaatgaaacc gacaatcggg cgatggggca agcgtccgcc
    17821 gctcagcgcc ggctcagcac ctggtccagc ggcttcctga cccgcgccgc tgccggtgta
    17881 cgggcgtcgg cggcgggata gcccgtgaca atcaggatga acggcttctc gcgctccggg
    17941 cgaccgcaga cacggttgag gaagcccatg ggcgagggcg tgtgggtgag cgtgacgagc
    18001 ccggcccgat gcagcgtggc gagcaggagg ccggtcgcga tgcccaccga ttcgccgaca
    18061 tagtagtgct tgcggcgtgc gccatcggcg gcgatcccgt ggcgctcggc gaagacggcg
    18121 atcagccagg gtgccgtctc gagaaagggc ttgcgccagt cggtgccaag cggcgcgagc
    18181 gcctcgagcc attccgggcc ggcgcgctcg gcatagaagg cccgctcctc cgcttcggcc
    18241 gcggcccgca gctcggcgcg cagctcgccc ttgccgatca ggcagaaatg ccagggctgc
    18301 tggttggcac cggagggtgc ggtggtcgcc gcccggatcg cggccaggac cacggcttcc
    18361 gggaccggct cgtcggcgaa gtcgcgcacc gtgcggcgat gcgcgagctc ggccgcgaag
    18421 ctctcggcgc gcgccaccat ctccgcttcg gggagacgtc ggtagtcgtc gaggggaacg
    18481 agctccatct tcccgccttc ctgcccgaca agccggagat cacgccagcc gctctacggt
    18541 cggccggccg gcagatgacc ttccttgatg tagatgaggc tcgcgtcgag cgcacgcagc
    18601 gagaattgcg agccggccgg ctggcgaaac caggcccagg cccgatgcag ccggcccacc
    18661 ttctcgatgc ggccgtcgac gaggaaggct tcgatgccgc cgggcccgac ctcccgatcg
    18721 accaccgtgc ccggggccag acgctcgagc cggacgacct cgtcccggtg ccggtgcaac
    18781 gggagaacct ccgcccccgg cgtcggagcg tgccatgcgt ccgggtgcgt ggtgtcgaga
    18841 cgcaccggtg ttag
//
LOCUS       DAOTFI010000080        18684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3564_length_18684_cov_6.304472, whole genome shotgun sequence.
ACCESSION   DAOTFI010000080 DAOTFI010000000
VERSION     DAOTFI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18684
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3564_length_18684_cov_6.304472"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="P5558_10015"
     CDS             <1..235
                     /locus_tag="P5558_10015"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRY24687.1"
                     /translation="TDTLFRHFADAEAECQRTLEHKLALPAYDQCLKASHTFNLLDAR
                     GVISVAERQAYIGRVRNLAKACAETWLASREHG"
     gene            228..2312
                     /gene="glyS"
                     /locus_tag="P5558_10020"
     CDS             228..2312
                     /gene="glyS"
                     /locus_tag="P5558_10020"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRY24688.1"
                     /translation="MAELLLELLSEEIPARMQRQAASELSTRLDQALAEGGVRGRLAH
                     CHATPRRLMVAFRDLPIVSADRAIERRGPRVDAPEKARDGFLKSLGVVDYTLGEVEDR
                     KGRFLVASSTEPGRSTVEIVAEAVPLILAGFPWPKSMRWGTGEARWVRPLQSILCLLD
                     GEVVPFEFAGIESGRQTFGHRFMAPDALVVRDVDDYRDHLIAARVMLDAADRRDVIEA
                     KAKELAVAAGYGVRPDPVLLDEIAGLVEWPVPLLGRIDADFMALPAEVLVTTMRANQK
                     YLALEDGAGRLAPAFVTVANIEAPDGGAAIIAGNERVLRARLWDARFFWEQDTRSTLE
                     ALLPKLDAMVFHAELGSMREKVDRLETLAVRLADSIPGADRLLVERAARLAKADLVSG
                     MVGEFPEVQGIMGGYYARAQGEADAVAQAIAEHYRPLGPGDACPTAPTSIAVALADKL
                     DTLTGFFAAGIRPTGSKDPFALRRAALGVIRLVIENGLRLDLGAVVELALGTHGQRFA
                     SVDAGGLARDLVAFLIDRLKVQQREQGVRHDLIEAVVVGRPDGDLVRVVARVAALRAF
                     IATSDGADLLAALRRAGNIVAIEEKKEGRPFTGPVDPTLLQEPAERRLFEELEAASAV
                     IRARLAAEDYAAAMAALARLRPAVDGFFECVMVNAGEPSVRANRLRLLGLLRDDLARI
                     ADFPVIDESAPI"
     gene            2338..5004
                     /gene="ppdK"
                     /locus_tag="P5558_10025"
     CDS             2338..5004
                     /gene="ppdK"
                     /locus_tag="P5558_10025"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRY24689.1"
                     /translation="MTKWVYGFGGGRADGGAKMKALLGGKGANLAEMSSLGLPVPPGF
                     TITTEVCTHFYAHGRSYPAALEAEVEAAIAKVEGELEMRFGDAGNPLLFSVRSGAPVS
                     MPGMMDTVLNLGLNDETVQGLVAASRDERFAYDSYRRFIQMYADVVLEVDHYLFEEAL
                     EAAKAEADCRFDNELDAAALQKLVATYKRIVRKQTGEAFPEEPRVQLWGAIGAVFGSW
                     QTPRAVTYRRLHDIPSAMGTAVNVQAMVFGNMGSDCATGVAFTRNPSTGERSVYGEFL
                     VNAQGEDVVAGIRTPQPLSRAMAESGSFSADESMETLLPDSYAELVSVFDRLEAHYRE
                     MQDVEFTIQRARLYVLQTRTGKRTAPAALRIAVDMAAEGLISEDEAILRIDAASLDQL
                     LHPTLDPDAAAEVIARGLPASPGAASGKIVFLADDATRAAQAGEDVILVRVETSPDDI
                     HGMHAARGILTSRGGMTSHAAVVARGMGKACICGVSELLISATEKTVTVRGRTLNEGD
                     WITIDGAAGIVMLGQVPTVDPVLSGDFATLMGWADKKRHLKVRTNAETPLDARAAVGF
                     GAEGIGLCRTEHMFFQEDRIVAMREMILSPDVEGRRRALAKIEPVQRDDFVELFEIMK
                     GLPVTIRLLDPPLHEFLPHAAAEDEAVAAATGVDIKLVRRRVAELREANPMLGLRGCR
                     LGILYPEIYEMQARAIFEAALVVFEKSGETVTPEVMIPLICSARELELIKARIVAVAE
                     AVEADRGRKLTYEIGTMIELPRAALRAGEIALHASFFSFGTNDLTQMTLGLSRDDAGR
                     FLDTYLHEEIFASDPFQSLDQDGVGELVQLAVERGRAARPDLKLGICGEHGGDPASID
                     FCHRQNLDYVSCSPYRVPIARLAAAQAALRGA"
     gene            5001..5969
                     /gene="meaB"
                     /locus_tag="P5558_10030"
     CDS             5001..5969
                     /gene="meaB"
                     /locus_tag="P5558_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRY24690.1"
                     /translation="MSLPETTLETLRAGGKAPLARCLADLESEAGRTRHRRLLDEAHA
                     APRGRVIGLTGPPGVGKSTLLARLIDRYRGTGATVGVLAVDPSSRRTGGAVLGDRTRF
                     DHDPEDGGVFVRSLAAGDRLGGLADATFPLMVVLRALFDVVLVETVGVGQSEIEIGEV
                     ADSVLLAIQPASGDSLQFIKAGIVEIPDIVVIGKADLGEIAARAQGEIEAALRHATPV
                     DPEWRPPVLRLSAVSGAGVDDLVAALARHAAHQAAAGRLEARRLAQSRSWLRATIRET
                     YGRRGLERLRPALEESFAGPPASPFLLLERLGQALDEVGLPEGGAG"
     gene            complement(5895..6812)
                     /locus_tag="P5558_10035"
     CDS             complement(5895..6812)
                     /locus_tag="P5558_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HRY24691.1"
                     /translation="MPSAALLDNRACLRLSGEDAMALLQGLVSNDLNRLTPDRAIYTT
                     LLTPQGKFLFDFFVHADGAALILETAGDRLADLERRLTLYRLRSDVTIEPVEPAPLVA
                     VLFGDGAAALADLPAEPGATREADGLTWTVDPRLPALGVWLRGAPEDVRARLDAAGLE
                     LVDATDFNRHRITLAVPEAGSDLIVDRSILLEAGLDQLNGVAFDKGCFVGQELTARTR
                     YRGLVKRRLVPVELVGASVEPGFAIMAGEVEAGEIRSTTGDLAMALLRLDRLGGALEA
                     GGGAVLPRPAFWQTDLIERLAETLEQEKG"
     gene            6945..8717
                     /locus_tag="P5558_10040"
     CDS             6945..8717
                     /locus_tag="P5558_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRY24692.1"
                     /translation="MMDYGLIGNCAINALIDASARIVWSCFPRFDSEPIFCRLLDGDR
                     DRGYFGVELEDCVSIEQAYVENTAVLRTEMRDARGAGIRILDFAPRFEQFGRMFHPTT
                     LVRIVEPLGEGPRIKIACRPLYNYGAQLPVITRGSNHIRYVDDIMALRLTTNAPLTYV
                     LDEITFHLDGTVALLLGADESLRSGLVETARDFLEKTTRYWLQVSGRLNIPFEWQEAV
                     IRSAITLKLCSFEETGAIIASPTTSLPETDGEGRNWDYRFCWLRDAFFVVRALNRLGY
                     VETMESHLRYLSDLVARSEDGYLQPVYGIGLEKRLTERVIDSLEGFLGNQPVRVGNQA
                     YEHDQHDGYGSVILAATQAFFDRRLRRPAGRATFERLELLGDKAWALHDVPDAGLWEF
                     RTKARVHTHSSVMCWAACDRLARIAARLELVEREIHWRGRADHIRAVIEERAWNPGLG
                     SYTASFDDDDIDASLLLIHEVGFLQGDDPRFAGTVKAVEERLKVGPYVYRYRSQDDFG
                     EPENAFLICSFWYVDALIALGRRDEARALFERLVACRNRLGLLSEHINRTTGELWGNF
                     PQTYSHVGLINCAMRLSRPWEDVV"
     gene            8836..10239
                     /gene="otsA"
                     /locus_tag="P5558_10045"
     CDS             8836..10239
                     /gene="otsA"
                     /locus_tag="P5558_10045"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HRY24693.1"
                     /translation="MARLVVVSNRVTPISGRQAASAGGLAVGVLAALRDSGGLWFGWS
                     GEIIETARGDDAGVPPARVFRTGKVTYGLIDLPEAEYEGFYAGFANRTLWPLFHYRLD
                     LTSFEHPWYATYRKVNQRFAEALRPLLKPDDVIWIHDYHLIPLATELRRAGCENRMGF
                     FLHIPFPAPEIYTSLPWHRQLAADLCNYDVLGFQTATDVRQFNNYVELEINGRADRKG
                     SVQALGRNLHVDAYPIGIDVDDVTEMAVSPEARRQAARLGSSLANRQLIIGVDRLDYT
                     KGIVERLRAFEMLLKANAVLRRQVTFVQISAPSRELVPEYLELRQQVELLSGHINGLY
                     SEADWVPLRYINRSFTRRTLMGFFRLSRVGLVTPLRDGMNLVAMEYVAAQNADDPGVL
                     VISRFAGCAQQLDGALVVNPYDVHHVADTLQAGLGMTLDERQDRWQRMMQAIRKNDII
                     TWRDRFLARLENRVGVG"
     gene            complement(10340..10669)
                     /locus_tag="P5558_10050"
     CDS             complement(10340..10669)
                     /locus_tag="P5558_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HRY24694.1"
                     /translation="MKQIYFIELGTGVDLRGGNPTKAAVRAVRDAIGRNLIPSVPQLT
                     GGDLDKLQVLVKLAVPVDEPIDEAAIREVLPVGTVRFEVTRGGMATDNGIGDRALIVN
                     AAVVVGI"
     gene            complement(10711..11037)
                     /locus_tag="P5558_10055"
     CDS             complement(10711..11037)
                     /locus_tag="P5558_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24695.1"
                     /translation="MTETGAQATVKGGMTQGQARFIEYAIITLAVVAVLMIFQPFSLT
                     LFSIGCIGVVVAGLAFNLIPFCRPGVPMRTLLRVIVIVLVILGIAALLGIGTANLYVW
                     YLGTLR"
     gene            complement(11034..12125)
                     /locus_tag="P5558_10060"
     CDS             complement(11034..12125)
                     /locus_tag="P5558_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24696.1"
                     /translation="MTIALRKLTKRFGSLTAVDAIDLEIGDGEVLCLLGPSGCGKTTT
                     LRMIAGLEWASSGDVLLAGKRVNDLAPRDRNVAMSFQFYALYPSLTVAENLAYPLYGE
                     GLGAAQIRAKVEQVARVLQLESIMGRKPGELAEGEKQRVAVGRSIIREPNCFLFDEPL
                     SRLDVQLREEMRGEIKEVLQGLRRATVIVTHDQLEALTMADRIAVMRAGVIEQVATPH
                     DIFQKPENLFVAGFIGTPAMNLIPAELTAFDAGNIARFAVEGGQTLAVEVDPAVARLR
                     SGAKVTLGVRPRNLEVVHEDVPDGLSSVIDIIEPMGAETLAHLNDGRHDIRVVTDWRT
                     PLEERDRVHVRFLPGSVHVFDADEKVVRA"
     gene            complement(12122..12988)
                     /locus_tag="P5558_10065"
     CDS             complement(12122..12988)
                     /locus_tag="P5558_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY24697.1"
                     /translation="MARPREIGKAGWGWTLFAGLISLVYFFPVLWIVLTAFKTRTDAL
                     AVPAKLFFTPTMQNFINVFYRESITAGAAQATEFPLYFFNSIFVSGTSVALAIVLGTL
                     AAYAFSRYPLRGNDTYLFIILTTRMMPPIVVIIPIFLMFRLTGLSGSYWGIILLYTAF
                     NIPFSIWLVKSFFDELNPEIEDAARMDGANERRVFFGVCLPQILAGLAATFVFGLILT
                     WNEFLFALLLTGVETRTVPVAMARTMGGEVGVDYGLLAAIVTLFLVPIFIVTYLLQNQ
                     LLRGVTFGTVKK"
     gene            complement(12988..13929)
                     /locus_tag="P5558_10070"
     CDS             complement(12988..13929)
                     /locus_tag="P5558_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY24698.1"
                     /translation="MAAASPHDRMAAPEGAGGFLAWLMAPERRGPLLVAPAVIVLFVM
                     NIFPLLWSFGLSFFNYRANRQDVPDFVGLRFYERVLSDPDVWDRFQTTAVIVGSSVVL
                     QLVIGFLLALMFAREFPLRRILLMLVLAPMMLSFVSVGVFFKLYYEPTFGLLSQFVST
                     FTGEPFVLLGTPTGAIVGIVVADAWMWSPFVMLLVLAGLVSVPKYLYEAAEIDRASWW
                     RRFRTITFPYIKSLLLLAVLFRTIETFKLFDIVYIITEGGPGTATETIAVFTYRVAFQ
                     FFRTSQSSALAYIILFVVIVLTNLYLYAVKRREQEVA"
     gene            complement(14019..15488)
                     /locus_tag="P5558_10075"
     CDS             complement(14019..15488)
                     /locus_tag="P5558_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY24699.1"
                     /translation="MRTIRDSMVLGVSAAAVATALLAGGTARAADITITMAAPDWPPT
                     RFMKEEFDRSYQSPDGHNTVLDIDFIPWPSFYERVAASLASGEQKYQMIVTDSQWLGA
                     FVEGGYYMDLTDLMTADPELTAIMQDLHPALVSAYSTYPHKSKDFIADNPFPAPDTRY
                     FGFPQFPDTYVTFYREDLFCHEGEAAAFQEKYGEALPCTYEDWQNVDWDTFRNVGEFF
                     RRAKGEPLGDGVADDDFYGIAYQAGKGYDFSTMAINAFIWQHGGSIWDETKAPEAQAE
                     GVVNSDVAVEALEHYLSLLQYMPPVAQTGQMDIFVIQDLYMQGKVAAIIDWVGLGEPV
                     LDPSSSVVADKSAFGPAPGLRKEDGSIDRTGNIGGQPFVLTTWNSDEVTNEALNVVKW
                     WLKPEVQKNFVAGGGQSGLVSVMADPEYNSLRPWNRAHVEMLDWQRDVWHVPDFFELL
                     TQQQEEYDRAITGQIDAQTALDSIARFQQQLLEDAGLIQ"
     gene            complement(15539..16645)
                     /locus_tag="P5558_10080"
     CDS             complement(15539..16645)
                     /locus_tag="P5558_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24700.1"
                     /translation="MAHLRVSDVARRFGAVTALDGMSFEVEEGEFYCLLGPSSAGKTT
                     TLRAIAGLEKLSRGRIEFAGRDVTTAPVQGRGMAMIFQTFALYPHLTVRQNFAYPLER
                     ERVDRAEIGRRVGEVSELLRVGHTLDRKPATLSGGEQQRIAIGRALIRRPKLLLLDEP
                     LTNLDAKLRHDTRAEFKRLHRELGMTMLYATPDQLEALTMGQRIGVMRGGRVVQIDTP
                     RALYEAPLDTYVAGLVGAPAMNLLPGRVTGGQGGLAVDFGVAGIDLAPGARDLAAGTE
                     LVVGLRPHDIRLGRDSARGRTVDAVVHLREPLGDVTVLDLRFGAIEARGAIEAKMVVR
                     EEQGALIPEGSAIEVTIDPADLHLFHAETGVRLA"
     gene            complement(16817..17443)
                     /locus_tag="P5558_10085"
     CDS             complement(16817..17443)
                     /locus_tag="P5558_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRY24701.1"
                     /translation="MTIEAHGAGQGNDTMAKKILMLVGEFSEEYEIFVFQQAMEAVGH
                     PVDVVCPDKKAGELIKTSLHDFEGDQTYTEKPGHFFKLNKTFAEVDTGSYDAVYVAGG
                     RGPEYIRIHPRVQAIVREFHEAKKPIFTICHGAQVLIAVDGVVSGKRVAALEYCEPEV
                     RLAGGIYVDVPPEGAISDGNLVSAKGWPGLAAFIRECLKVLGTEIRHA"
     gene            complement(17458..18624)
                     /locus_tag="P5558_10090"
     CDS             complement(17458..18624)
                     /locus_tag="P5558_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY24702.1"
                     /translation="MPRPLRVAVIGYGFMGKAHSNAYANVNHYFPEVPFRIERQVLCG
                     RDATKAQAFADDWGYAGVASDWREVVARDDVDLVDICVPNNLHEAIATAAAEAGKWVV
                     TEKPLAMSVAEGERMVAAVEQAGVPNMVWYNYRRVPAISLAKAMVDEGRLGRAYHYRA
                     QFLQDWTIAEDLPQGGAALWRLDAAAAGSGVTGDLLAHNIDTAMWLNGPITRVMAETE
                     TFVKERLHQETGKVAPVGIDDACLFMCRFANGSLGLFESTRYARGHKALKTLELNGAD
                     GSVRFDLHDPQYLDFFEYRSKETGDKTAGHLNGWRRIHVTNHEHPYMSHWWVPGLTVG
                     YEATFVHALADFLAGAEDKTFKGPTFRDALDTQKVCEAVLRSAETGKWEATGLG"
BASE COUNT         2903 a         6335 c         6521 g         2925 t
ORIGIN      
        1 cacggacacg ctctttcgcc acttcgccga cgccgaggcc gagtgccagc gcacgctcga
       61 gcacaagctg gccctgccgg cctatgacca gtgcctgaag gcgagccaca ccttcaacct
      121 gctcgatgcg cggggtgtga tcagcgtcgc cgagcggcag gcctatatcg gccgggtgcg
      181 caacctggcc aaggcctgcg ccgagacctg gctggcgagc cgcgagcatg gctgagctgc
      241 tgctggagct gctgtcggag gagattccgg cgcggatgca gcggcaggcc gcatcggagc
      301 ttagcacccg tctcgaccag gccctcgccg aggggggcgt gcggggccgg ctggcccatt
      361 gccatgccac cccgcggcgc ctcatggtgg cgttcaggga tctgccgatc gtctcggccg
      421 accgggccat cgagcggcgc ggcccgcgcg tcgatgcgcc ggaaaaggcg cgggacgggt
      481 tcctgaagag cctgggggtg gtcgactaca cgctcggcga ggtcgaggac aggaagggtc
      541 gctttctcgt cgcgagctca accgagccgg ggcggtcgac ggtcgagatc gtcgccgaag
      601 ccgttccgct catcctcgcc ggctttccct ggcccaagtc gatgcgctgg ggcacgggcg
      661 aggcgcgctg ggtgcggccg ctgcagtcga tcctctgcct cctcgacggc gaggtcgtgc
      721 ccttcgagtt tgccgggatc gagagcggcc ggcagacctt cggccatcgc ttcatggcgc
      781 ccgacgccct cgtcgtgcgc gacgtcgacg actaccgcga ccatctgatc gcggcccggg
      841 tgatgctgga cgccgccgat cggcgggacg tcatcgaggc gaaggcgaag gagctggcgg
      901 tggccgccgg ctacggcgtg cggcccgatc cggtcctgct cgacgagatc gcgggcctgg
      961 tcgagtggcc ggtgccgctg ctgggccgga tcgacgcgga tttcatggcg ctgccggccg
     1021 aggtgctggt gaccaccatg cgcgccaacc agaaatacct ggcgctcgag gacggggcag
     1081 ggcggctggc gccggccttc gtcaccgtcg ccaatatcga ggctcccgat ggcggggccg
     1141 cgatcatcgc cggcaacgag cgggtgctgc gcgcacggct ctgggatgcg cgcttcttct
     1201 gggagcagga tacgagatcg accctcgagg cgctcctgcc gaagctcgac gccatggtct
     1261 tccatgccga gctcggctcg atgcgcgaga aggtcgaccg gctcgagacg ctcgcggtgc
     1321 ggctcgccga ttcgatcccc ggcgccgacc gcctgctcgt cgagcgggcg gcacggctcg
     1381 ccaaggccga tctcgtctcc ggcatggtcg gcgagttccc cgaggtgcag ggcatcatgg
     1441 gcggctacta tgcgcgcgcc cagggcgagg ccgacgccgt ggcccaggcc atcgccgagc
     1501 attatcgccc gctcgggccc ggcgatgcct gccccaccgc cccgaccagc atcgccgtgg
     1561 cgctcgccga caagctcgac acgctcaccg gcttcttcgc cgccggcatc cgcccgaccg
     1621 gctcgaagga cccgttcgcg ctgcggcgtg ccgcgctggg cgtgattcgc ctggtcatcg
     1681 agaacgggct gcggctcgat ctgggcgcgg tcgtcgagct ggcgctcggc acccacgggc
     1741 agcgcttcgc cagcgtcgat gcgggtgggt tggcccggga cctggtcgcc ttcctgatcg
     1801 atcggctcaa ggtgcagcag cgcgagcagg gcgtgcggca cgacctgatc gaggcggtcg
     1861 tggtcgggcg gccggacggc gatctcgtgc gcgtggttgc ccgcgtcgca gcacttcggg
     1921 cctttatcgc cacgtcggac ggggcggatc tcctggcagc gctccgccgc gccggcaaca
     1981 tcgtcgcgat cgaggagaag aaggagggcc ggcccttcac cggcccggtc gacccgacgc
     2041 tcctgcagga gcccgccgag cgacggctct tcgaggagct cgaggcggca agcgcggtca
     2101 tccgggcacg gctcgcggcc gaggactatg ctgcggcgat ggcggcactt gcccgattgc
     2161 ggccggcggt cgatggattt ttcgagtgcg tgatggtcaa tgccggggaa ccctcggtgc
     2221 gcgccaaccg cttgcgcttg ctgggcttgt tgcgagacga cctcgcccgg atcgccgact
     2281 tcccggtcat cgacgaatcg gccccgatct gatggtcgcg tcaggagacg aaccaggatg
     2341 acgaagtggg tctacggctt cggcggcggt cgcgccgacg gtggtgcgaa gatgaaggcg
     2401 ctgctcggcg gcaagggcgc caatctcgcg gagatgtcgt cgctcggcct gccggtgccg
     2461 ccgggcttca ccatcacgac cgaggtctgc acccacttct acgcgcatgg ccgaagctac
     2521 ccggcggcgc tcgaggccga ggtggaagcc gccatcgcga aggtcgaggg cgagctcgag
     2581 atgcgcttcg gcgatgccgg gaatccgctc cttttctcgg tccgctcggg tgctcccgtt
     2641 tcgatgcccg ggatgatgga cacggtgctc aatctcgggc tcaacgacga gacggtgcag
     2701 ggcctcgtcg ccgcgagccg ggacgagcgc ttcgcctatg acagctaccg ccggttcatc
     2761 cagatgtatg ccgacgtggt gctggaggtc gaccactacc tcttcgagga ggcgctcgag
     2821 gcggccaagg ccgaggccga ctgccgcttc gacaacgagc tcgacgcggc ggcgctgcag
     2881 aagctggtgg cgacctacaa gcgcatcgtc cgcaagcaga ccggcgaggc gtttccggag
     2941 gagcccaggg ttcagctctg gggtgccatc ggtgccgtct tcggctcctg gcagacgccg
     3001 cgcgccgtca cctatcgccg cctgcacgac atcccgagcg ccatgggcac cgcggtcaac
     3061 gtccaggcga tggtgttcgg caacatgggg agcgactgcg ccacgggcgt cgccttcacc
     3121 cgcaacccgt cgaccggcga gcgcagcgtc tatggcgagt tcctggtcaa cgcccagggc
     3181 gaggacgtgg tcgccggcat ccgcacgccg cagcccttga gccgggccat ggcggagagc
     3241 ggcagcttct ccgccgacga gtcgatggag acgctgctgc cggattccta tgccgagctc
     3301 gtcagtgtgt tcgaccggct cgaggcgcat tatcgcgaga tgcaggacgt cgagttcacg
     3361 atccagcgcg cgaggctcta cgtactgcag acccggaccg gcaagcgcac ggccccggcg
     3421 gcgctcagga tcgccgtcga catggcggcc gaggggctga tcagcgagga cgaggcgatc
     3481 cttcggatcg acgccgcctc gctcgaccag ctcctgcacc cgaccctcga tcccgacgcg
     3541 gcagccgagg tgatcgcccg tggcctgccg gcctcgccgg gtgccgcctc gggcaagatc
     3601 gtcttcctcg ccgatgacgc gaccagggcg gcccaggccg gcgaggacgt catcctcgtg
     3661 cgcgtcgaga cctcgcccga cgacatccac ggcatgcatg cggccagggg catcctcacg
     3721 tcccggggcg gcatgaccag ccacgcggcg gtggtggccc gcggcatggg caaggcctgc
     3781 atctgcggcg tcagtgagct cctcatctcg gcgaccgaga agaccgtgac ggtgcgcggc
     3841 cggacgctca acgagggcga ctggatcacc atcgacgggg ccgccggcat cgtcatgctc
     3901 ggccaggtgc cgacggtcga tcccgtgctc tccggcgact tcgccacgct gatgggctgg
     3961 gccgacaaga agcggcacct caaggtccgc accaatgccg agaccccgct cgatgcccgg
     4021 gcggccgtcg gcttcggcgc cgagggcatc gggctctgcc gcaccgagca catgttcttc
     4081 caggaggacc gcatcgtcgc catgcgcgag atgatcctga gcccggacgt cgaggggcgg
     4141 aggagggcgc tggcgaagat cgagccggtg cagcgggacg acttcgtcga gctcttcgag
     4201 atcatgaagg ggctgccggt caccatccgc ctgctcgacc cgccgctgca cgagttcctg
     4261 ccgcatgccg cggccgagga cgaggccgtg gcggcggcga ccggcgtcga catcaagctg
     4321 gtgcgccggc gggtcgccga gctccgggaa gccaacccga tgctcgggct gcgcggctgc
     4381 cggctcggca tcctctatcc cgagatctac gagatgcagg cccgggcgat cttcgaggcg
     4441 gcgctcgtgg tgttcgagaa gtccggcgag acggtgacgc ccgaggtgat gatccccttg
     4501 atctgcagcg cgcgcgagct cgagctcatc aaggcgcgga tcgtcgccgt ggccgaggcg
     4561 gtcgaggcgg atcgcggccg caagctcacc tacgagatcg gcaccatgat cgagctgccg
     4621 cgggcggcgc tcagggccgg cgagatcgcc ctgcacgcga gcttcttcag cttcggcacc
     4681 aacgacctga cgcagatgac gctcggcctc agccgcgacg atgccggccg cttcctcgac
     4741 acctacctgc acgaggagat cttcgcctcc gacccgttcc agtcgctcga ccaggacggg
     4801 gtgggcgagc tggtgcagct ggccgtcgag cgcggccggg ccgcgcggcc cgatctcaag
     4861 ctcggcatct gcggcgagca tggcggcgat ccggcctcga tcgatttctg ccaccggcag
     4921 aatctcgact acgtctcctg ctcgccctac cgcgtgccga tcgcgcggct cgccgctgcc
     4981 caggcggcgc tgcgcggggc ttgagcctgc cggagacgac gctcgagacg cttcgcgccg
     5041 gcggcaaggc tccgctcgcg cgctgcctcg ccgatctgga gagcgaggcc ggccgcaccc
     5101 gccaccggcg tctgctggat gaggcccatg ccgccccgcg cggccgggtg atcggcctca
     5161 ccgggccgcc cggcgtcggc aagtcgaccc tgctggcccg gctgatcgac cggtatcggg
     5221 gcacgggtgc gacggtgggg gtgctcgcgg tggacccgtc ctccaggcgg accggcggcg
     5281 cggtgctcgg cgatcgcacc cgcttcgacc acgatcccga ggatggcggc gtcttcgtcc
     5341 gctcgctcgc cgccggcgac cggctgggcg gcctcgccga cgcgacattt ccgctgatgg
     5401 tcgtgctgcg cgccctcttc gacgtggtgc tcgtcgagac cgtcggcgtg ggccagtcgg
     5461 agatcgagat cggcgaggtg gccgacagcg tcctgctcgc catccagccg gcctccggcg
     5521 acagcctgca gttcatcaag gcggggatcg tcgagatccc ggacatcgtg gtcatcggca
     5581 aggccgatct cggcgagatc gctgcccggg cgcagggcga gatcgaggcg gcgcttcgcc
     5641 atgcgacgcc cgtcgacccc gagtggcggc cgcccgtcct gcggctctcg gcggtcagcg
     5701 gggcaggggt cgacgacctc gtcgcggcct tggcgcgcca tgctgcccat caggccgccg
     5761 caggcaggct ggaagcccgc cggctcgccc agagccggag ctggcttcgt gcgacaattc
     5821 gcgagaccta tggcaggcgc gggctcgagc ggctgcggcc ggcgctggag gaaagcttcg
     5881 ccggccctcc ggcctcaccc tttctcctgc tcgagcgtct cggccaggcg ctcgatgagg
     5941 tcggtctgcc agaaggcggg gcggggtagg acggcgccgc cgccggcttc gagcgcgcca
     6001 ccgagccggt cgaggcgaag gagcgccatg gcgaggtcgc ccgtggtgct gcggatctcg
     6061 cccgcctcga cctcgccggc catgatggcg aagcccggct cgacggaggc gccaacgagc
     6121 tcgaccggca ccagccggcg cttgacgagg ccccgataac gggtgcgggc ggtgagctcc
     6181 tggccgacga agcagccctt gtcgaaggcg acgccgttga gctggtcgag ccccgcctcg
     6241 agcaggatcg acctgtcgac gatgaggtcg ctgccggcct cggggacggc caaggtgatg
     6301 cggtgccgat tgaagtccgt cgcatccacc agctccagcc cggccgcgtc gagccgggcg
     6361 cgcacgtctt cgggtgcgcc gcgcagccag acgccgagcg ccggcagccg ggggtcgacc
     6421 gtccaggtca gcccgtccgc ctcgcgcgtg gcgccgggct cggcgggcag gtcggccagt
     6481 gccgctgcgc catcgccgaa gagcacggcg acgagcggtg ccggctcgac cggctcgatc
     6541 gtcacgtcgg agcgcagccg atagagggtc aggcggcgct cgagatccgc cagccgatcg
     6601 ccggcggtct cgaggatcag cgcggcaccg tcggcatgca cgaaaaaatc gaaaagaaac
     6661 ttgccctgcg gtgtcagcag cgtcgtgtag atcgcccgat caggcgtcag cctgttcaga
     6721 tcgttgctga cgagaccctg caggagcgcc atcgcatcct cgccggaaag ccgcaggcag
     6781 gcgcgattgt cgagcagggc cgccgacggc atcgatttct tcatcttttg caagcatcat
     6841 ccccgcggtc ggatggccta gagatgggcg ttcggcatgg ctcggtaaag ggtggaccct
     6901 gccgatccgc gtggaaggag accggtgggc gagggctgct gcacatgatg gactacggac
     6961 tgatcggcaa ttgcgcgatc aatgcgctga tcgacgcgag cgccaggatc gtctggagct
     7021 gctttccgcg gttcgacagc gagccgatct tttgccggct gctcgatggc gatcgcgacc
     7081 gcggctattt cggcgtcgaa ctcgaggact gtgtcagcat cgagcaggcc tatgtcgaga
     7141 acaccgccgt gctgcgcacc gagatgcgcg acgcgcgtgg cgccggcatc aggattctcg
     7201 acttcgcgcc gcgcttcgag cagttcggcc ggatgttcca tccgaccacc ctcgtccgca
     7261 tcgtcgagcc gctgggcgag gggccgcgca tcaagatcgc ctgccgaccc ctctacaatt
     7321 atggcgcgca gctgccggtg atcacccggg gcagcaacca catccgttat gtggacgaca
     7381 tcatggcgct caggctcacc accaacgcgc cgctgaccta tgtgctcgac gagatcacct
     7441 ttcatctcga cggcaccgtg gcgctgctgc tcggcgccga cgagagcctg cgcagcggcc
     7501 tcgtcgagac ggcccgggat ttcctcgaga agaccacccg ttactggctg caggtctccg
     7561 gccgcctgaa catcccgttc gagtggcagg aggcggtgat ccgctcggcc atcacgctca
     7621 agctctgcag cttcgaggag accggggcga tcatcgcctc gccgaccacc tcgctgccgg
     7681 agacggacgg ggaggggcgc aactgggatt atcgcttctg ctggctgcgg gacgccttct
     7741 tcgtcgtccg ggcgctcaac cgcctgggct atgtcgagac catggagagc catctgcgct
     7801 atctctccga cctcgtcgcc cggagcgagg acggctacct ccagccggtc tacggcatcg
     7861 gcctcgagaa gcgcctgacc gagcgcgtca tcgacagcct cgaaggcttt ttgggcaacc
     7921 agccggtgcg cgtcggcaac caggcctacg agcacgacca gcatgacggc tatggcagcg
     7981 tcatcctggc ggcgacccag gccttcttcg accgtcgcct gaggcgcccg gcgggccgcg
     8041 ccaccttcga gcggctcgag cttctgggcg acaaggcctg ggcgctgcat gacgtgccgg
     8101 atgccgggct ctgggagttc cgcaccaagg cccgggtgca cacccattcc agcgtcatgt
     8161 gctgggcggc ctgcgaccgg ctcgcccgga tcgccgcccg gctcgagctg gtcgagcgcg
     8221 agatccactg gcgcggccgg gcggaccaca tccgcgccgt catcgaggag cgcgcctgga
     8281 atcccgggct cggcagctac accgccagct tcgacgacga cgacatcgat gcgagcctcc
     8341 tgctcatcca cgaggtcggc ttcctgcagg gtgacgaccc gcgtttcgcc ggcacggtca
     8401 aggcggtgga ggaacggctg aaggtcgggc cttatgtcta ccgctatcgc agccaggacg
     8461 atttcggcga gccggagaac gccttcctca tctgcagctt ctggtatgtc gacgcgctca
     8521 tcgccctcgg ccggcgcgac gaggcgaggg cgctcttcga gcggctggtc gcctgccgca
     8581 accgcctcgg cttgctgtcg gagcacatca accgtacaac cggggagctt tggggcaact
     8641 tcccgcagac ctacagccat gtcgggctga tcaattgcgc catgcggctg agccgtccgt
     8701 gggaggatgt cgtctgatcc aagaggggtc ggcgggcggg cagcgggtcc ggctcctggc
     8761 cgagcggaac gtccgatgcc attcgggcgt tacgccgaga cgggttgtgg caacgacatc
     8821 cagggaggaa gcagcttggc gcggctggtg gtcgtctcga accgggttac cccgatcagt
     8881 ggccggcagg cggcttcggc cggcggcctc gcggtcggcg tgctggcggc gctgcgcgac
     8941 agcggcgggc tctggttcgg ctggagcggc gagatcatcg agaccgcgcg cggcgacgat
     9001 gccggggtgc caccggcccg ggtcttccgc accggcaagg tcacctatgg gctgatcgac
     9061 ctgcccgagg ccgagtacga gggcttctat gccggcttcg ccaaccgcac gctctggccg
     9121 ctcttccact accgcctcga cctgacctcc ttcgagcatc cctggtacgc cacctaccgc
     9181 aaggtcaacc agcgcttcgc cgaggcgctc cggccgctgc tcaagcccga cgacgtcatc
     9241 tggatccacg actaccacct gattccgctc gccaccgagc tgcgccgggc cggctgcgag
     9301 aaccggatgg gcttcttcct ccacatcccg ttcccggcgc cggagatcta caccagcctg
     9361 ccctggcacc ggcagctcgc cgccgatctc tgcaattacg acgtgctggg cttccagacc
     9421 gcgacggatg tccgccagtt caacaattac gtcgagctcg agatcaacgg ccgcgccgac
     9481 aggaagggaa gcgtccaggc gctcggccgc aacctgcatg tcgacgccta tccgatcggc
     9541 atcgacgtcg acgacgttac cgagatggcg gtctcgccgg aggcgaggcg gcaggcggca
     9601 cggctcggct cgagcctcgc caaccgccag ctcatcatcg gcgtcgaccg cctcgactac
     9661 accaagggca tcgtcgagcg gctgcgcgcc ttcgagatgc tcttgaaagc caatgccgtg
     9721 ctgcgccggc aggtgacgtt cgtgcagatc tcggcaccgt cgcgcgagct ggtgcccgaa
     9781 tatctcgagc tccgccagca ggtcgagctg ctctcgggcc acatcaacgg cctctacagc
     9841 gaggccgact gggtgccgct gcgctacatc aaccgcagct tcacccggcg cacgctgatg
     9901 ggcttcttca ggctcagccg ggtcggcctc gtgacgccgt tgcgcgacgg catgaacctc
     9961 gtcgccatgg agtacgtggc ggcgcagaac gccgacgacc ccggcgtgct cgtcatctcg
    10021 cgcttcgccg gctgcgcgca gcagctcgac ggtgccctcg tggtcaatcc ctacgacgtg
    10081 caccatgtgg cggacaccct gcaggcgggg ctcggcatga cgctcgacga gcgccaggac
    10141 cgctggcagc ggatgatgca ggcgatccgc aagaacgaca tcatcacctg gcgcgaccgc
    10201 ttcctcgcac ggctcgagaa ccgcgtcggc gtcggctgag ccgtgccgac cacccgggcg
    10261 acgagacggg ggcggcggtc cgggcgaagc cccgcgggtg gtcggattat cggataagaa
    10321 tgattatgac aaatatcgct cagatgccga cgacgaccgc ggcgttgacg atcagcgccc
    10381 ggtcgccgat gccgttgtcg gtggccatgc cgcctctcgt cacctcgaag cgcaccgtgc
    10441 cgaccggcag cacctcgcgg atggcggcct cgtcgatcgg ctcgtccacc ggcaccgcca
    10501 gcttcaccag cacctgcagc ttgtccaggt cgccgccggt gagctgcggg acggacggga
    10561 tgaggttgcg cccgatggcg tcgcgcaccg cccgcaccgc ggccttggtg ggattgccgc
    10621 cgcgcaggtc gacgccggtg ccgagctcga tgaaatagat ctgcttcatg gtcgcttcgc
    10681 ttccctcgat tctgtcgttc gcccgggcat tcagcgcagg gtgccgagat accagacgta
    10741 gagattggcc gtgccgatgc cgaggagcgc cgcgatcccg agaatgacca gcacgatgac
    10801 gatcacccgc agcagcgtgc gcatcggcac gcccggccgg cagaacggga tcaggttgaa
    10861 ggccaggccc gccaccacca cgccgatgca gccgatcgag aagagcgtca gcgagaacgg
    10921 ctggaagatc atcaagacgg ccacgacggc gagcgtgatg atcgcgtact cgatgaagcg
    10981 cgcctggccc tgggtcatgc cgcccttgac ggttgcctgt gcgccggtct cggtcatgcc
    11041 cgcaccacct tctcgtccgc gtcgaacacg tgcaccgagc ccggcaggaa gcggacatgc
    11101 acccggtcgc gctcctcgag cggtgtgcgc cagtcggtga ccacgcggat gtcgtgccgc
    11161 ccgtcgttca ggtgcgccag ggtctcggcc cccatgggct cgatgatgtc gatcaccgag
    11221 gagagcccgt cgggcacgtc ctcgtgcacc acctcgagat tgcgcggccg cacaccgagc
    11281 gtgaccttcg cgccggaccg caggcgggcg accgccgggt cgacctcgac cgccagcgtc
    11341 tgcccgccct cgaccgcgaa acgggcgata ttgcccgcgt cgaaggcggt gagctcggcc
    11401 gggatcaggt tcatcgccgg ggtgccgatg aagccggcga cgaagaggtt ctcgggcttc
    11461 tggaagatgt cgtgcggtgt cgccacctgc tcgatcaccc cggcccgcat gaccgcgatg
    11521 cggtcggcca tggtcagcgc ctcgagctgg tcgtgggtga cgatcaccgt cgcccggcgc
    11581 aggccctgca gcacctcctt gatctcgccg cgcatctcct cgcggagctg cacgtcgagc
    11641 cgcgacaggg gctcgtcgaa gaggaagcag ttgggctcac ggatgatcga gcggccgacg
    11701 gcgaccctct gcttctcgcc ctcggcgagc tcgccgggct tgcgccccat gatcgattcg
    11761 agctgcagca cccgcgccac ctgctcgacc ttggcgcgga tctgcgctgc cccgagcccc
    11821 tcgccataga gcggataggc gagattctcg gccacggtca ggctcgggta gagggcgtag
    11881 aactgaaagc tcatggcgac gttgcggtcg cgcggggcga ggtcgttgac ccgcttgccg
    11941 gcgagcagca cgtcgcccga gctcgcccat tcgagcccgg cgatcatcct gagcgtcgtc
    12001 gtcttgccgc agcccgaggg gccgagcagg cagagcacct cgccgtcgcc gatctcgaga
    12061 tcgatggcgt cgaccgcggt gagcgagccg aaccgcttgg tgagctttcg gagggcgatc
    12121 gtcatttctt gaccgtgccg aaggtgacgc cgcgcaggag ctggttctgc aggagatagg
    12181 tgacgatgaa gatcggcacg aggaagagtg tgacgatggc ggcgagcagg ccgtagtcga
    12241 cgcccacctc gccgcccatg gtgcgggcca tggcgaccgg caccgtgcgc gtctcgaccc
    12301 cggtcagcag cagcgcgaag aggaactcgt tccaggtcag gatcaggccg aagacgaagg
    12361 tggcggcgag gccggccagg atctgcggca ggcagacgcc gaagaagacc cgccgctcgt
    12421 tggccccgtc catccgcgcc gcgtcctcga tctccgggtt gagctcgtcg aagaagctct
    12481 tcaccagcca gatggagaac gggatgttga aggcggtgta gagcaggatg atgccccagt
    12541 agctgccgga gagcccggtc aggcggaaca tcaggaagat cgggatgatg acgacgatgg
    12601 gcggcatcat ccgggtggtc aggatgatga agagataggt gtcgttgccg cgcaaggggt
    12661 agcgcgagaa ggcgtaggcg gcgagcgtgc cgaggacgat ggcgagcgcc acgctggtgc
    12721 ccgagacgaa gatcgagttg aagaaataga gcgggaactc ggtcgcctgc gccgccccgg
    12781 ccgtgatgct ctcgcggtag aagacgttga tgaagttctg catcgtcggc gtgaagaaga
    12841 gcttggccgg caccgccagc gcgtccgtgc gcgtcttgaa ggcggtcagc acgatccaga
    12901 ggaccgggaa gaagtagacc aggctgatca gccccgcgaa cagggtccag ccccagccgg
    12961 ccttgccgat ctcgcgcggt ctggccatca ggcgacctcc tgctcgcgcc gcttgacggc
    13021 gtagagatag agattggtga gcacgatcac cacgaagagg atgatgtagg cgagtgcgga
    13081 ggactggctg gtgcggaaga actggaaggc cacacgatag gtgaagaccg cgatcgtctc
    13141 ggtcgcggtg ccgggcccgc cctcggtgat gatgtagacg atgtcgaaga gcttgaacgt
    13201 ctcgatcgtc cggaagagca cggcgagcag gagcaggctc ttgatgtagg gaaaggtgat
    13261 ggtgcgaaag cgccgccacc aggaggcgcg atcgatctcg gccgcctcgt agaggtattt
    13321 cggcaccgag acgaggccgg cgagcaccag cagcatgacg aagggcgacc acatccaggc
    13381 atcggccacg acgatgccga cgatcgcccc ggtcggcgtg ccgagcagga cgaagggctc
    13441 gccggtgaag gtcgagacga actggctgag caggccgaag gtcggctcgt agtagagctt
    13501 gaagaagacg ccgaccgaga cgaaggacag catcatcggc gccagcacca gcatcaggag
    13561 aatgcggcgg agcgggaact cgcgggcgaa catcagcgcc aggaggaagc cgatgacgag
    13621 ctgcaggacg acgctcgagc cgacgatgac ggccgtggtc tggaagcggt cccagacgtc
    13681 cgggtcgctc agcacgcgct cgtagaagcg caggccgacg aagtccggca cgtcctgccg
    13741 gttggcgcgg tagttgaaga agctgaggcc gaaggaccag agcagcggga agatgttcat
    13801 gacgaagagc acgatgaccg ccggggccac gagcagcggc ccgcgtcgct cgggcgccat
    13861 cagccaggcg aggaacccgc cggcaccctc gggcgctgcc atgcggtcat gcggcgatgc
    13921 tgctgccatc gtcgtccggt ctcatcggca aggggttgga atgaagaaga aggaaggcgc
    13981 ggcggcccgg gcggaaccgg gccgccgcgc cggccggcct actggatcag gccggcatcc
    14041 tcgaggagtt gctgctggaa gcgggcgatg ctgtcgagcg ccgtctgcgc gtcgatctgg
    14101 ccggtgatcg cccggtcgta ctcctcctgc tgctgggtca ggagctcgaa gaagtccggc
    14161 acgtgccaga cgtcacgctg ccagtcgagc atctcgacat gcgcccggtt ccagggccgc
    14221 aggctgttgt actcgggatc ggccatgacg ctcaccagcc ccgactggcc gccaccggcg
    14281 acgaagttct tctgcacctc gggcttcagc caccacttga cgacgttgag ggcttcgttc
    14341 gtgacctcgt cgctgttcca ggtggtcagc acgaagggct ggccgccgat attgccggtc
    14401 cggtcgatcg agccgtcctc cttgcgcagg cccggcgccg ggccgaaggc cgacttgtcg
    14461 gcgacgaccg agctgctcgg gtcgagcacc ggctcgccga gaccgaccca gtcgatgatc
    14521 gccgccacct tgccctgcat gtacaggtcc tggatgacga agatgtccat ctgcccggtc
    14581 tgggccaccg gcggcatgta ctggagcagc gagaggtagt gctcgagcgc ctcgaccgcg
    14641 acatcggaat tgacgacgcc ttcggcctgc gcctcgggcg ccttggtctc gtcccagatc
    14701 gacccgccat gctgccagat gaaggcattg atcgccatgg tcgagaagtc gtagcccttc
    14761 ccggcctggt aggcgatgcc gtagaaatcg tcgtcggcga cgccatcgcc gagcggctcg
    14821 cccttggccc gccggaagaa ctcgccgacg ttcctgaagg tgtcccagtc gacgttctgc
    14881 cagtcctcgt aggtgcaggg cagcgcctcg ccgtacttct cctggaacgc cgccgcctcg
    14941 ccctcgtggc agaaaaggtc ctcgcggtag aaggtgacgt aggtgtccgg gaactgcggg
    15001 aagccgaaat agcgcgtgtc cggcgccggg aagggattgt ccgcgatgaa gtccttggac
    15061 ttgtgcggat aggtcgaata ggccgagacc agcgccgggt gcaggtcctg catgatggcg
    15121 gtgagctcgg gatcggcggt catgagatcg gtcagatcca tgtagtagcc gccctcgacg
    15181 aacgcaccca gccactggct gtcggtcacg atcatctggt atttctgctc gcccgaggcg
    15241 agcgaggcgg cgacccgctc gtagaagctc ggccacggga tgaagtcgat gtcgagcacc
    15301 gtgttgtgcc cgtcaggcga ctggtaggag cggtcgaact cctccttcat gaaccgggtc
    15361 ggcggccagt cgggtgccgc catggtgatg gtgatgtcgg ctgcccgggc ggtgccgccg
    15421 gcgagcagcg ccgtggccac ggctgccgcc gagaccccca gcaccatgct gtccctgatc
    15481 gttcgcattc ttttcgttct cctcgttgcc ttgggaaggt ggttggtcgt cggtgtcgtc
    15541 aagcgagccg gaccccggtc tcggcgtgaa agaggtggag gtccgccggg tcgatggtca
    15601 cctcgatggc gctgccttcg gggatcaggg ccccctgctc ctcgcgcacc accatcttgg
    15661 cctcgatcgc gcccctggcc tcgatggcgc cgaagcgcag gtcgagcacc gtcacgtcgc
    15721 ccaaaggctc gcgcagatgc acgacggcat cgacggtcct gccgcgcgcg ctgtcccggc
    15781 ccagccggat gtcgtgcggc ctgaggccga ccaccagctc ggtgccggcg gcgagatccc
    15841 ttgcaccggg cgcgaggtcg atgccggcca cgccgaaatc gacggcgagc cccccctgcc
    15901 cgccggtcac ccggcccggc agcaggttca tcgccggcgc acccacgagc cccgccacat
    15961 aggtgtcgag cggcgcctcg tagagggcgc gcggcgtgtc gatctggacc acccggccgc
    16021 cgcgcatgac cccgatccgt tggcccatgg tgagcgcctc gagctggtcc ggcgtcgcat
    16081 agagcatggt catgccgagt tcgcgatgca ggcgcttgaa ctcggcgcgc gtgtcgtggc
    16141 gcagcttggc gtcgagattg gtcaagggtt cgtcgagcag gaggagcttc gggcggcgga
    16201 tcagcgcccg gccgatggcg atccgctgct gctcgccgcc ggagagcgtc gccggcttgc
    16261 gatcgagcgt atggccgacc cgcagcagct cggaaacctc gccgacgcgc cggccgatct
    16321 cggcccggtc gacccgctcg cgctcgagcg ggtaggcgaa gttctggcgg acggtcagat
    16381 gcgggtagag ggcgaaggtc tggaagatca tggccatgcc gcggccctgc accggtgccg
    16441 tggtgacgtc gcggccggcg aactcgatcc ggccccggct cagcttctcg agcccggcga
    16501 tggcgcgcag cgtggtcgtc ttgccggccg agctggggcc gagcaggcaa tagaactcgc
    16561 cttcctcgac ctcgaagctc atgccgtcga gggcggtgac ggccccgaaa cggcgcgcca
    16621 catcactgac ccgaagatgt gccaagattc gcctgcctcc cgcttctgtc gaccgcccgg
    16681 aacactggcc ggccggctct tgcggctgcg ccgaggctta gctgaaggca ccaggcctgc
    16741 caagaactta actgccggag agggccgccg ggcggccgcc cggcatcagc cgggtacggc
    16801 cgctgccggg ccgggctcag gcgtgccgga tctccgtgcc cagcaccttc aggcactccc
    16861 tgatgaaggc ggcgaggccg ggccagccct tggcggagac gaggttgccg tcgctgatgg
    16921 cgccttccgg cggcacgtcg acatagatgc ccccggcgag gcgcacctcg ggctcgcaat
    16981 actcgagggc ggcgacgcgc ttgccgctga ccacgccatc caccgcgatc agcacctggg
    17041 caccgtggca gatggtgaag atcggcttct tcgcctcgtg gaactcgcgc acgatcgcct
    17101 gcacccgggg atggatgcgg atatattccg gcccgcggcc gccggcgacg tagaccgcgt
    17161 cgtagctgcc ggtgtcgacc tcggcgaagg tcttgttcag cttgaagaaa tggcccggct
    17221 tctcggtgta ggtctggtcg ccctcgaaat cgtgcagcga ggtcttgatc agctcgcccg
    17281 ccttcttgtc cgggcagacg acatccaccg gatgccccac cgcctccatc gcctgctgga
    17341 acacgaagat ctcgtattcc tcgctgaact cgccgaccag catcaggatc ttcttcgcca
    17401 tcgtgtcgtt cccctgcccg gccccgtggg cctcgattgt catgaaattc tgccggctca
    17461 gccgaggccc gtggcttccc acttgccggt ttcggccgag cgcagcaccg cctcgcagac
    17521 cttctgggta tcgagcgcgt ccctgaaggt cggccccttg aaggtcttgt cctcggcacc
    17581 ggcgaggaag tccgccagcg catggacgaa ggtcgcctcg tagccgacgg tcaggcccgg
    17641 cacccaccag tggctcatat aggggtgctc gtgattggtg acgtggatcc ggcgccagcc
    17701 attgaggtgg cccgcggtct tgtcgccggt ttccttcgag cggtactcga agaagtcgag
    17761 atattgcggg tcgtggaggt cgaagcgcac cgagccgtcg gcgccgttca gctcgagcgt
    17821 cttcagggcc ttgtggccgc gggcgtagcg cgtgctctcg aacaggccga gcgagccgtt
    17881 ggcgaagcgg cacatgaaga ggcaggcgtc gtcgatgccg accggggcga ccttgccggt
    17941 ctcctggtgc agccgctcct tcacgaaggt ctcggtctcg gccatcaccc tggtgatcgg
    18001 gccgttcagc cacatcgccg tgtcgatgtt gtgggcgagc aggtcgccgg tgacgccgga
    18061 gcccgcggct gccgcgtcga ggcgccagag ggcggcgccg ccctgcggca ggtcctcggc
    18121 gatcgtccag tcctgcagga actgggcgcg gtagtggtag gcgcggccga gccgcccctc
    18181 gtcgaccatc gccttggcga ggctgatggc cggcacgcgg cggtagttgt accagaccat
    18241 gttgggcacg ccggcctgct cgaccgcggc caccatccgc tcgccctcgg cgacgctcat
    18301 cgccaggggc ttctcggtga ccacccactt gccggcctcg gccgccgccg tggcgatcgc
    18361 ctcgtgcagg ttgttgggca cgcagatgtc gacgagatcc acgtcgtcgc gggccacgac
    18421 ctcgcgccag tcgctcgcca ccccggcgta gccccagtcg tcggcgaagg cctgcgcctt
    18481 cgtggcatcc cggccgcaca gcacctgccg ctcgatgcgg aagggcacct ccgggaagta
    18541 gtggttgacg ttggcgtagg cattggaatg ggccttgccc atgaacccgt aaccgatcac
    18601 ggcgacccgc agcggcctcg gcatgttgct ttcctccccg gtggttcgag cacgcttgca
    18661 cctgccctta gcacgaccgc cgcc
//
LOCUS       DAOTFI010000081        18584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3585_length_18584_cov_6.635868, whole genome shotgun sequence.
ACCESSION   DAOTFI010000081 DAOTFI010000000
VERSION     DAOTFI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18584
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3585_length_18584_cov_6.635868"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="P5558_10095"
     CDS             complement(<1..520)
                     /locus_tag="P5558_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY24703.1"
                     /translation="MNSGKRILIVDDDEAFRGELAEQLGLHESCEVLQAGTGKEGLEL
                     AKAHPLDALILDVGLPDTDGRDLCRLIRKAGIKAPVIMLTGHDSDSDTILGLDSGAND
                     YVGKPVRLGVLMARLRAQLRQFELSDDATFQIGPYAFKPAAKMLVTAADKKIHLTEKE
                     TAILKYLFRVGDR"
     gene            772..2301
                     /locus_tag="P5558_10100"
     CDS             772..2301
                     /locus_tag="P5558_10100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY24704.1"
                     /translation="MFLILYLMALAALLLHAPPDLLDPANRPFFVVIGVIGIWRYGWG
                     AVHFLRAQYYKRIEFRGLRRRATALLPAPAADSADETRAGPEVFIVVTSFRIRAETTA
                     TVYRGAIAEACRHDGPAVIVASIVEMADQRFIKQLFYSMAPPEHVRLIFVRTAGLGKR
                     HGLAVALRAVARSRPANDAVVALVDGDTVLIPGTFERCLPFFRLLPEIGALTTDEEPV
                     IQSGALLRQWYMLRFAQRQLLMCSMGLSRRLLTLTGRMSFFRVRIATDPSFVDTLRDD
                     SIEHWRLGRIPLLTGDDKSTWFWLLQRNWDMLYVPDVRILTLEDPPNGRFLIATTRLM
                     RRWFGNMLRASGRAIALGPRRVGLFPWWCLIDQRVSMWTPLLLPLSALALTLATGPTV
                     LYAYAIWVMTTRLLQSLLLLGVRDRIGTLFPLLILYSQVYGAMVKTWVLFHPHRQSWT
                     RQGIVAPAPAGISGRLQAALSHYTHALAMLALILAVAVFTGLIETPSPRTVALTLARL
                     F"
     gene            2327..3385
                     /locus_tag="P5558_10105"
     CDS             2327..3385
                     /locus_tag="P5558_10105"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRY24705.1"
                     /translation="MQPQVVHEAEVHRQHVRLKIPINVEIDGSAFTVDDWSMGGFGIF
                     SEMTSRQPGERFPVKLIFPFEDFEVTLRLDCQMVYILEESTRFGCRFLGLSQGQLALF
                     RYLVDAYLSGEIVSGGDILAIAGRENSAAVRERGLPYNPYAEAEGVGRRVKQIAGYTL
                     LGVASLGLAALIFTGVQERFFTVRAESAVIETPVVRLRAPDAGIVTPATLPNILQPGT
                     PVARIETADGRFVHLESPCDCVLYEWLVMPGQFTQQGEDVAVLVTANQPLLVRAQVTF
                     EQANRLAVGDLALIDVPGRSDGMRGQVESIDFRPSLRRIDETVGTAPSRRLAQVTIRP
                     DQPFEFEDLGSIVGVSFP"
     gene            3464..3925
                     /locus_tag="P5558_10110"
     CDS             3464..3925
                     /locus_tag="P5558_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HRY24706.1"
                     /translation="MLKRLAAFLVAGCLLLGSALADEETLRSLALLLGLEDAYGFAET
                     VLTLRREGELPDHYVTKGEARDLGWRPGDDLCRSAPGRVIGGDRFGNREGRLPDRPGR
                     RWHEADLDFDCGRRGARRLVFSSDGLVFVTLDHYETFYEIPAEATGEEDRQ"
     gene            3922..4197
                     /locus_tag="P5558_10115"
     CDS             3922..4197
                     /locus_tag="P5558_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRY24707.1"
                     /translation="MTTARLDGRQRSKRAVLARLASELAFPPHFRPNLDALYDVLTTD
                     LEGPITIHWRATEHARRALAGDFEAIARTLAAAAAERDDLTLRLTTP"
     gene            4832..5176
                     /locus_tag="P5558_10120"
     CDS             4832..5176
                     /locus_tag="P5558_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY24708.1"
                     /translation="MLQFDAATARLLDDGYLGSDVARRRLANLAALAPAPGERIADIG
                     CGTGLLTRDLARAVGETGCITAIDPSAEMRAACERRCAACSHVKVVEGSAEVLPLDDG
                     SVDGAVALQVFE"
     gene            5313..5630
                     /locus_tag="P5558_10125"
     CDS             5313..5630
                     /locus_tag="P5558_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24709.1"
                     /translation="MGRMLRLWDRHLVERRVPALLPPLLRAAGFEVEAIVPELCQDNI
                     FRADGLARLMTHLIGAYVGENDLAEPRDVGAWIAEQQRMAEEGRFFFAITHYVVAARK
                     NPG"
     gene            complement(5670..6842)
                     /locus_tag="P5558_10130"
     CDS             complement(5670..6842)
                     /locus_tag="P5558_10130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY24710.1"
                     /translation="MAVDARRTETAGGLGFLRQLFAERPAAPATLDDPLGDATLLALR
                     RHGIRPTPESFTLWYRHLAGERPDLSRRLKELEARGEPFDAGLIGVLHERYFGADQAA
                     LLVAEASRNALRLLARLESDLAGAEADAAARAERVGRLGRKLEGAPGEAKDGAASAAS
                     AEVHARYGEIRARVAELARETEGMRVAAGRLQRRVVEGAAEITQLRAALGASEGADEV
                     DPVSGVARQRRLERALERAVAAAEAADRPLVYMIVDLDRFAAFNEACGRRLGDLMLRA
                     VARRIGLALKRQDTIGRLDGAAFGVVLVDTGLDDATALAERLCRDIADLTLESGETGA
                     AIPAVTVAIGVALSHPGEPLQRLVGRADRARRQARDAGGNRALSERSIAVVGRPKA"
     gene            complement(7004..7390)
                     /locus_tag="P5558_10135"
     CDS             complement(7004..7390)
                     /locus_tag="P5558_10135"
                     /inference="COORDINATES: protein motif:HMM:NF006040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HRY24711.1"
                     /translation="MSFLKWLTDNRLGTHLFTRWRGERVGEDALGNVYYRRPGAADWR
                     DERRWVVYAGDGELEASSVPAGWNAWLHKNREKAPSELPLAERFWEKPHAANPSGTVG
                     AYLPPGHEKRGGQRDHATGDYEAWRP"
     gene            8260..11862
                     /locus_tag="P5558_10140"
     CDS             8260..11862
                     /locus_tag="P5558_10140"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRY24712.1"
                     /translation="MKIERAFTKAGASPYAAIAFRSASSEIRNPDGSVVFAATELEVP
                     AGWSQVAVDVLAQKYFRKAGVPARLKPVREKGVPAWLCRRVPDEAALAALPEAERTTG
                     ETSAKQVFDRLAGAWTYWGWKGRYFSTEADARGFYDELRCMLARQYAAPNSPQWFNTG
                     LHWAYGIDGPAQGHFYVDETTGKVHASTSAYERPQPHACFIQSIQDDLVNEGGIMDLW
                     VREARLFKYGSGTGTNFSNLRGEGERLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVIVDVDHPDIEAFIDWKVIEEQKVAALVAGSRLLQRHANAIMEACHGAADVPQD
                     KRCDPLANPFLKRAIRDARRALLPEGSIQQAILLAAQGYTAVEVPVYDADWDSQAYLT
                     VAGQNSNNSVRISDAFYQAVQQDREWPLTARISGKVAKTVRAKALWDKLCFAAWASAD
                     PGVQYDTTINDWHTCPADGRINASNPCSEYMFLDDTACNLASLNLMQFRAADGSFLVD
                     DFEHAVRLWTLVLEISVMMAQFPAARIAELSYRFRTLGLGYANLGGLLMASGIPYDSD
                     EGRAYCGAITALMTGVAYRVSAELAAELGAFPGYAPNRESTLRVLRNHRRAAHGEARG
                     YEDLATLPVPLDAGHCPSEPLALAARRAWDEAVAMAEQHGVRNAQVSVIAPTGTIGLV
                     MDCDTTGIEPDFALVKFKKLAGGGFFKIINRMVPQALATLGYSKAQVEAITRYAVGHG
                     TLQHSPGIDHAALRAKGFTDEVLKKLEAGLPTTFDIRYAFNRFSLGDAFCRDVLGFTD
                     EQLGDVRFDMLQSLGFSRDAIEQANAFCCGTMTIEGAPELRPEHLAVFDCANPCGRIG
                     RRYLSASSHIRMMAAAQPFISGAISKTINMPARATVEDCREAYLEGWRLGVKALALYR
                     DGSKLSQPLSSMLADVIEDEAEDDSAARSQVERIVEVTERVVERFVNGRRKLPSRRKG
                     YTQKAIVGGHKLYLRTGEYADGALGEIFVDMHKEGAAFRSLMNSFAISVSIGLQYGVP
                     LEEFVEAFSFTRFDPSGVVQGNDTIKMATSVLDYIFRELAISYLERTDLANVDPAELR
                     HDAIGAGTKEGDMGENVAQIASAGFVRGNLRLLAGGGAVMEPAASGGGSRQAGGSAAA
                     GLTTLSRDSRRQQAEIARLQGYEGDPCSACGNFTLVRNGTCLKCDTCGSTTGCS"
     gene            12309..13001
                     /locus_tag="P5558_10145"
     CDS             12309..13001
                     /locus_tag="P5558_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY24713.1"
                     /translation="MHRSDEVRPFIEKQLAAGTVIFWAGDPSDAVALIIDGEVEVLAE
                     QVNGLVRIGKHGNGAYVGDKDVLAERPRRFTFRTTRPTRVEMLSGHLFMERMRAARAA
                     AEPRAEALVRLLPASTLSAGLLPEEGLCLTSFPFSVGREGSDGKEDAASDAQARLVIA
                     ESPPYRLSRRHFTILREGDIIRVVDPGSRLGTLVDGQRVGLEPVTLAAGSTHEIIAGG
                     ASSPYRFQLAID"
     gene            13058..13621
                     /locus_tag="P5558_10150"
     CDS             13058..13621
                     /locus_tag="P5558_10150"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HRY24714.1"
                     /translation="MSVVHELSRRTLLLGACLSWALAATPAAEAATGAEIDAKVDLAL
                     AQLLRESDTARALADKAVAILVFPDIVKGGFGIGGQFGEGALRRNGKTEGYYNIAAAS
                     FGLQIGAQSFSQAIFFMTEEALAYLDSSKGFEIGADANVALANEGLGYDVTSSTVQDP
                     IIAFVFGQKGLMAGLTIEGSKITKLAR"
     gene            complement(13729..14550)
                     /locus_tag="P5558_10155"
     CDS             complement(13729..14550)
                     /locus_tag="P5558_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24715.1"
                     /translation="MAAGDRPSRLPRRLERVLRWAMPLGLVLVVLGLWELGVRWHDTP
                     HYILPGPVLVARTLVADWHTLYPSLLVTLRVTFSALAVAVVGGVGLAVLFTLSRWIEL
                     SLFPFAVILQVTPIIAIAPLILIYVENTHLALLICAWIVAFFPILSNTTLGLNSADHN
                     LVDLFRLYKASRWQMLRFLRLPSAMPYFLGGLRIAGGLALIGAIVAEFAAGTAGTGSG
                     LAYRILEASYRLNIPRMFAALFLISMAGILIFLALTLLSHLLLRKWHESAVKRDG"
     gene            complement(14550..15326)
                     /locus_tag="P5558_10160"
     CDS             complement(14550..15326)
                     /locus_tag="P5558_10160"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24716.1"
                     /translation="MRADTPSGATADALLELRGVSKTFPNGTVALDGLDLDVGRHELL
                     CMLGPSGCGKSTALRLIAGLGEASAGRLAWPNGGSAGDIGFVFQEPTLMPWATVFDNV
                     HLPLRLQGRGRRRAGEAVREALALVGLDDFAGAYPRELSGGMRMRVSIARALVTRPRL
                     LLMDEPFAAVDEITRHRLNHDLLDLWQGQGWTVVFVTHSVYEAVFLASRVVVMSARPG
                     RIVAGFEIAEPYPRDDAFRLSESYLHYCRQASAALQQAVG"
     gene            complement(15323..16339)
                     /locus_tag="P5558_10165"
     CDS             complement(15323..16339)
                     /locus_tag="P5558_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24717.1"
                     /translation="MFRPVTLLLSSFSLAALLAAPALALDAVTLGTNWKAQAEHGGFY
                     QALATGLYEAHGLEVTIRPGGPQVNHPQLLAAGRIDFCLAGSMTNAFNYLTADVPMVT
                     VAAIFQKSPLVLMAHPDRGIETPADLAHMPILISTAGRQTFWDWLKITYGLEDGQLRP
                     YTFNAAQFIADPGVAQQGYLTAEPFAVAREGGFAPRVLLLADFGYDSYASMIETSWRL
                     VEENPDLVQRFVDASILGWVSYLHGDPTPGNALIKAANPDMTDAQIAYAIEQMTARGI
                     VESGDALTLGIGAMTDARWRSFLASLAAVGLYPADLDLSRAYTLAFVNRQVGLALGRE
                     LTGG"
     gene            complement(16466..16542)
                     /locus_tag="P5558_10170"
     tRNA            complement(16466..16542)
                     /locus_tag="P5558_10170"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16506..16508),aa:His,seq:gtg)
     gene            16849..17187
                     /locus_tag="P5558_10175"
     CDS             16849..17187
                     /locus_tag="P5558_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRY24718.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEVGLKGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIELVVDDAIVDRAVEAIQQSARTDRIGDGKIFVLPVEDAVRI
                     RTGERGADAV"
     gene            17314..>18584
                     /gene="glnA"
                     /locus_tag="P5558_10180"
     CDS             17314..>18584
                     /gene="glnA"
                     /locus_tag="P5558_10180"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRY24719.1"
                     /translation="MTLGCKSAADVMKYVKDNGVEMIDLRLIDVPGIWQHISYPAAIF
                     DEGNIEAGLGFDGSSVRGFAQIQASDMLLRPDVTTAFLDPFTKHKTLVMICAVADPVT
                     GELYSRDPRGIAIKAEEYLKSTGIGDTAYFGPEAEFFVFSDVRMGQGVNYGTYQVDSP
                     EGHWNTMTDEGPNLGYKVPPKGGYFPCAPTDSLNDLRAEMALVMQSIGLTVECHHHEV
                     ATAGQCEIDMRFDSLLAMADHQMKYKYVVKNVARAAGMTATFMPKPLYGDNGSGMHCH
                     QSIWNGSTPLFADEAGYAGLSDMARHYIGGLLRHAPSILAFAAPTTNSYRRLVPGYEA
                     PVNLAWSLRNRSAACRIPMYSNSPKAKRVEFRCPDPSANPYLAFAAMLMAGIDGIKNK
                     IDPGAPIDKNIYDLPPEEAAEIPKVPGQLADA"
BASE COUNT         2862 a         6497 c         6414 g         2811 t
ORIGIN      
        1 cccggtcgcc gacccggaag agatatttga ggatcgcggt ttccttctcg gtgagatgga
       61 tcttcttgtc ggcggccgtc accagcatct tcgccgcggg cttgaaggca tagggcccga
      121 tctggaaggt cgcgtcgtcg gacagctcga actgacggag ctgggcgcgc aggcgcgcca
      181 tcagcacgcc gagccgcacc ggcttgccga catagtcgtt ggcgccgctg tccagcccga
      241 ggatcgtatc ggaatcgctg tcatggccgg tcagcatgat caccggcgcc ttgatcccgg
      301 ccttgcggat cagccggcag aggtcgcgcc cgtcggtgtc cggcaggccc acgtcgagga
      361 tcagggcgtc gagcggatgg gccttggcga gctcgaggcc ctccttgccg gtgccggcct
      421 gcaacacctc gcagctctcg tgcaggccga gctgctcggc gagctcgccg cggaacgcct
      481 cgtcatcgtc gacgatcagg atgcgcttgc cactgttcat gtcgcttcgc caatcgtgct
      541 gtggagccgc cctcacccgc gagactaccg acgcccgtgc ggaagcgaaa gagcgcccga
      601 tggccgcgcg tccctttggg cgcttgtcgc ccggccgcct tgcggctatc gcaatcacgc
      661 agcacgtgcg gaaagggtta acctctttcg tactatgatc gatccgcgcg gctcgttccc
      721 cgcgtggcgg aatggccgcc gttccggcga tcgaggcgac gaccgaccct gatgttcctc
      781 attctctacc tcatggccct ggcggccctc ctgctgcacg cgcctccgga tctcctcgat
      841 ccggccaacc ggccgttctt cgtggtgatc ggggtgatcg ggatctggcg ctatggctgg
      901 ggtgcggtgc acttccttcg ggcccagtac tacaagcgga tcgagttccg cggcctgcgc
      961 cggcgcgcca cggcgctgct gccggcgcca gccgccgaca gcgccgacga aacgcgggcg
     1021 gggcccgagg tcttcatcgt cgtcaccagc ttccgcattc gcgccgagac gaccgcgacc
     1081 gtctatcgcg gcgccatcgc cgaggcctgc cggcacgacg gcccagcagt gattgtggcc
     1141 tcgatagtcg agatggccga ccagcgcttc atcaagcagc tcttctactc gatggcgccg
     1201 cccgagcatg tccggctgat cttcgtgcgc acagccggtc tgggcaagcg gcacggcctg
     1261 gccgtggcgc tgcgcgccgt cgcccgcagc cggccggcga acgacgcggt cgtggccctg
     1321 gtcgacggcg acaccgtcct gatcccgggc acgttcgagc gctgcctgcc gttctttcgc
     1381 ctgctgcccg agatcggcgc cctgacgaca gacgaggagc ccgtcatcca gagcggcgcg
     1441 ctcctgcgcc aatggtacat gctgcgcttc gcccagcggc agctcctgat gtgctccatg
     1501 ggcctctcgc gcaggctctt gacgctcacc ggccggatgt ccttcttccg cgtgcggatc
     1561 gccaccgatc ccagcttcgt cgacacgctg cgcgacgaca gcatcgagca ctggcgcctc
     1621 gggcgcattc cgctgctcac cggcgacgac aagtcgacct ggttctggct cctgcagcgc
     1681 aactgggaca tgctctatgt cccggatgta cgcatcctca ccctcgagga tccgccgaac
     1741 gggcgctttc tgatcgccac cacccggctg atgcgccgct ggttcggcaa catgctgcgc
     1801 gccagtggcc gggccatagc gctcgggccg cggcgggtcg gcctctttcc ctggtggtgc
     1861 ctgatcgacc agcgcgtctc gatgtggacg ccgctgctgc tgcccctctc ggcgctggcg
     1921 ctcacgctcg ccaccggccc gaccgtgctc tatgcctacg ccatctgggt catgaccacg
     1981 cgcctcttgc agtcgctgct gctgctcggc gtgcgcgacc gcatcggcac gctcttcccg
     2041 ctgctcatcc tctacagcca ggtctacggc gccatggtca agacctgggt tctcttccat
     2101 ccgcaccgcc agagctggac gcgccagggg atcgtcgcac cggcgccggc cgggatcagc
     2161 gggcggttgc aggcggcgct ctcacactat acccatgcgc tcgccatgct cgccctgatc
     2221 ctcgccgtgg cggtcttcac cgggctgatc gagacaccgt cgcccaggac ggtcgctctg
     2281 acccttgccc gcctcttctg aacccgccgg tattctggaa cgctccatgc agccacaggt
     2341 cgtccacgag gccgaagtcc accgccagca cgtccggctg aagatcccga tcaatgtcga
     2401 gatcgacggc tcggccttca ccgtcgacga ctggtcgatg ggcggcttcg gcatcttctc
     2461 cgagatgacc tcgcgccagc cgggcgagcg ttttcccgtc aagctgatct tcccgttcga
     2521 ggacttcgag gtcacgctcc ggctcgactg ccagatggtc tacatcctcg aggagagcac
     2581 gcgcttcggc tgccggttcc tcggcctgag ccagggtcag ctcgccctct tccgctatct
     2641 ggtcgatgcc tatctctccg gcgagatcgt cagtggcggc gacatcctgg ccatcgccgg
     2701 gcgcgagaac tcggccgccg tgcgcgagcg cggcctgccc tacaacccct atgccgaggc
     2761 cgagggcgtc ggccgccggg tcaagcagat cgccggctac acgctcctcg gtgtcgccag
     2821 cctcggcctc gcggcgctca tcttcaccgg ggtccaggaa cgcttcttca cggtgcgggc
     2881 ggagagcgcc gtgatcgaga cccccgtcgt ccgcctcagg gccccggatg ccggcatcgt
     2941 gacgccggcc accttgccca acatcctcca gccgggcacg ccggtcgccc gcatcgagac
     3001 cgccgacggg cgcttcgtgc atctcgagag cccctgcgac tgcgtgctct acgagtggct
     3061 cgtcatgccg ggccagttca cccagcaggg cgaggacgtg gccgtgctgg tcaccgccaa
     3121 ccagccgctc ctggtccgcg cgcaggtgac gttcgagcag gcgaaccggc tcgcggtcgg
     3181 cgatctcgcc ctgatcgacg tgccgggccg gtccgatggc atgcgtggcc aggtcgagag
     3241 catcgacttc cggccgagcc tgcgccgcat cgacgaaacg gtcggaacgg ccccgagccg
     3301 ccgcctcgcc caggtcacca tccgccccga ccagcccttc gagttcgagg acctgggctc
     3361 catcgtcggc gtcagcttcc cgtgaggcgc ggctgcccga gccgggcaag gccggcggcg
     3421 gggcgtctcg accgccggtt cgaccactga ccggaaagtc ctgatgctca agcgcctcgc
     3481 tgccttcctc gtcgccggct gcctcctgct cggcagcgcg ctcgccgacg aggagacgct
     3541 gcgcagcctg gcgctgctgc tcggcctcga ggacgcctac ggcttcgccg agacggtcct
     3601 gaccctgcgg cgcgagggcg agctgccgga ccactacgtg accaagggcg aggcgcgcga
     3661 cctcggctgg cggccgggcg acgatctctg ccgctcggcg cccggccggg tgatcggcgg
     3721 cgaccgcttc ggcaatcgcg aggggcggct gccggaccgg ccgggccggc gctggcacga
     3781 ggccgacctc gacttcgact gcggccggcg cggcgcccgg cggctggtct tctccagtga
     3841 cggcctcgtc ttcgtcaccc tcgaccacta cgagaccttc tacgagatcc cggccgaggc
     3901 tacgggcgag gaggaccggc aatgaccacc gcccgcctcg acggccggca gcggagcaag
     3961 cgggcggtgc tggcgaggct cgccagcgag ctcgccttcc cgccgcattt ccgccccaat
     4021 ctcgacgcgc tctacgacgt gctgaccacc gacctcgagg ggccgatcac catccactgg
     4081 cgggccacgg agcatgcccg gcgtgccctg gccggcgatt tcgaggccat cgcccgcacg
     4141 ctcgccgcgg ccgccgcgga acgggacgac ctgacgctcc gcctcaccac gccctgacga
     4201 agatcgccga aaagccgccg ggcaacggga tcgaacaggt cggcatgtag gacttatagc
     4261 ctatatcggc gccgcgcgca agcctgcgac gggcggccgc gggttggttc ggcaatttgc
     4321 gacttcgacg gcccggcacg tcgggcgcgc ccgtcaaaaa cggccgaaaa ctgttcttga
     4381 tcagaatgat gcaggctcgc gggttcgttt gcccgagctc gggttcgatc gtcgaagaag
     4441 cgggttcgtt cgtccgaatc ccgggttcgc tcgtcgattc ggtgggttcg ctcgtcgccg
     4501 cctgtcgcgg aatccgcgac agacatgggc gagagtcgcc gattctgcga catcacgatt
     4561 ccttatgtcg cggattccgc gacagctcgg ccggcgctgt cgcggaatct gcgacagccc
     4621 gaggccggat gtcgcggaat ctgcaacaac ccgagcgatt caggaggtgg agggccaccc
     4681 ggtgacgggc tcgaccgggt cctcgggccg aaggcataag cgttcgtgat gatgatggcg
     4741 aggtggacgc ggtcgtgccc atgggcgatg ctgccgatcg aggcagccga acggcgagag
     4801 aaacgccacc ttctggcgag ggggagccac catgctgcag ttcgacgccg caaccgcgcg
     4861 acttctcgat gacggctatc tgggctcgga tgtcgcgcgc aggcgcctcg cgaacctcgc
     4921 cgccctggcg ccggcgcccg gcgagcggat cgccgacatc ggctgcggca ccggcctgct
     4981 gacccgcgac ctcgcccgcg cggtcggcga gaccggctgc atcaccgcca tcgatccgag
     5041 cgcggagatg cgcgcggcct gcgagaggcg ctgcgccgcg tgctcccatg tgaaggtggt
     5101 cgagggctct gcggaggtcc tgccactcga cgacggcagt gtcgacggcg cggttgccct
     5161 gcaggtcttc gagtaagtcg acgacattgg cgcggcattg gccgagaccc accgcgtctt
     5221 gcggccgggc gggcggctgg tcatcggcga cctgccccaa gacagccaaa ctgggggaca
     5281 gccacgtctg gcacgccgat gatccggacc gcatgggccg catgctgcgc ctctgggacc
     5341 ggcatctggt cgaacgccgt gtcccggcct tgctgccgcc tttgctccgg gcggccggct
     5401 tcgaggtcga ggcgatcgtg ccggagctct gccaggacaa catctttcgg gccgatggcc
     5461 tggcgcgcct gatgacccac ctgatcggcg catatgttgg agagaacgac ctcgccgagc
     5521 cgcgggacgt cggggcatgg atcgccgagc agcagcgaat ggccgaagag gggcgcttct
     5581 ttttcgccat cacccactat gtcgtggcgg cacgcaagaa tccggggtga gtggcgcgca
     5641 tctttcgatg tggcgcagcg agcgtcccgt caggccttgg ggcggccgac cacggcgatc
     5701 gagcgttcgg aaagagcgcg gttgccgccg gcgtcgcggg cctggcggcg ggcccggtcg
     5761 gcgcggccga cgaggcgctg caggggctcg cccggatgcg agagggcgac gccgatggcg
     5821 acggtgacgg ccgggatcgc ggcgcccgtc tcgccgctct cgagcgtcag gtcggcgatg
     5881 tcgcgacaga ggcgctcggc cagggcggtg gcgtcgtcga gcccggtgtc gacgaggacg
     5941 acgccgaagg cggcaccgtc gagtcggccg atcgtgtcct gccgcttcaa ggcgaggccg
     6001 atgcgccgcg ccacggccct gagcatgagg tcgccgagcc ggcggccgca cgcctcgttg
     6061 aaggcggcga accggtcgag gtcgacgatc atgtagacga gcggccggtc ggccgcttcg
     6121 gccgcggcga cggcgcgctc gagcgcgcgc tcgagccgcc gctgcctggc gacaccgctc
     6181 accggatcga cctcgtcggc gccctcgctc gccccgagcg ccgcgcggag ctgggtgatc
     6241 tcggcggcgc cctcgaccac ccggcgctgc agccggccgg cggccacgcg catgccctcg
     6301 gtctcgcggg cgagctcggc gacgcgcgcg cggatctccc cgtagcgggc atgcacctcg
     6361 gccgaggccg cgcttgcggc cccatccttc gcttcgcccg gcgccccctc cagcttcctg
     6421 ccgagccggc cgacgcgctc cgcccgggcc gcggcatcgg cttcggcacc ggcgagatcc
     6481 gactccagcc gcgccagcag ccgcagcgcg ttgcgactcg cctcggcgac caggagggcc
     6541 gcctggtcgg cgccgaaata gcgctcgtgg agcacgccga tcaggcccgc gtcgaagggc
     6601 tcgccgcgcg cctccagctc cttcaggcgg cgggacagat ccggccgctc gcccgccaga
     6661 tgccggtacc agagcgtgaa cgattccggc gtcggccgga tgccgtgccg gcggagcgcc
     6721 agcagcgtcg cgtcgccgag cggatcgtcg agcgttgccg gtgctgccgg gcgctcggcg
     6781 aagagctgcc gcaggaagcc caatccgccg gccgtctcgg ttcgccgcgc gtccaccgcc
     6841 atccgctctc catgctgcac ggatcggacg acactgtcag atgtggatga agaaagggtg
     6901 aaaccccgcc ttttccgcgg acttatccac aaggaatcag ccgggctgag ggtcgactga
     6961 ggcgcttgag tcgaggctca gcccgtcgcc cggggccgcc cggtcagggc cgccaggcct
     7021 cgtagtcgcc ggtcgcatgg tcgcgctgcc cgccgcgctt ctcgtggccg ggcggcaggt
     7081 aggccccgac cgtgcccgac gggttggcgg cgtgcggctt ctcccagaag cgctccgcca
     7141 gcggcagctc gctcggtgcc ttctcgcggt tcttgtgcag ccaggcgttc cagccggccg
     7201 gcacggagct cgcctcgagc tcgccgtccc cggcatagac cacccagcgg cgctcgtccc
     7261 gccagtccgc cgccccgggc cgccggtagt agacattgcc gagcgcgtcc tcgccgaccc
     7321 gctcgccccg ccaccgcgtg aagaggtggg tgccgagccg gttgtcggtg agccacttga
     7381 ggaaggacac gagcagggtc tccgcgcctg ggatggtcga tcggggaagg cgggcgcggc
     7441 actgccggcc gccgcccccg ggcgccctcc ttatgcgcag catcgccccg gccgtccagc
     7501 cgcgaagcga cgcacggcgg cgacggtccc gaccgtgacg gccgcgcggt ggacagcctg
     7561 tggaagaatc ccgaccagga tgccttgtgg atcattgccc cctgtggaca agccgggaca
     7621 agcacaagct gtggataaga ggggagcgac aggcggattg agcgacactc agcaggctca
     7681 tcgggcatca atcgaacgaa ccgggaacgc gcacggccgg ctctttcgga cttgtccaca
     7741 ggttcctgca aggcgttttc cggtggcgcc ggcgcgctcg ctgagtggca ttgagcccgc
     7801 cccgcagtca gcggggcgct gacccccctc tcgcgggaaa agcgagttcc tgcccgtggc
     7861 ccgcgccggt ccggcgatgg cagggcggca acaccagggg cggcccgggt ggaaagggcg
     7921 cgaagaacac aatatcttgt tgtggcgcac cggcgaattc gtatatctgg tcaggctagg
     7981 ctgtgctggc cgccgctcga gagcggcagg caatggccgg ggcggcgagt tgcaggcgtg
     8041 ggcacggcgg agcggcgaga ttttcgtggg tcctagccgc ccccgccgag gctttctgtt
     8101 gtgctccggc gcgggtggct ctaaactgag cgttgaatga tcctgagatc aatcggcgct
     8161 gatcgacgag ccttcgcgcg gacggcgggc ggctgcaagc gggtttgacg aacgagacga
     8221 gaacatccta gtctcgcagc gacgaggaga tcggtgtcga tgaagatcga gcgggccttt
     8281 accaaagccg gagcatcgcc ctacgcggcg atcgcgttcc gttccgcttc gagcgagatc
     8341 cgcaacccgg acgggtcggt ggtgttcgcg gcgacggagc tcgaggtgcc ggccggctgg
     8401 tcgcaggtcg ccgtcgacgt gctggcgcag aagtatttca ggaaggccgg cgtcccggcg
     8461 cggctgaagc cggtcaggga gaagggtgtc ccggcctggc tctgccgccg ggtgcccgac
     8521 gaggcggcgc tggccgccct gcccgaggcc gagcgcacca ccggcgagac cagcgccaag
     8581 caggtcttcg accggctggc cggggcctgg acatattggg gctggaaggg gcgctacttc
     8641 tcgaccgagg cggatgccag gggcttctac gacgagctgc gctgcatgct ggcgcgccag
     8701 tacgcggccc ccaactcgcc gcagtggttc aacaccggcc tgcactgggc ctatggcatc
     8761 gacggcccgg cgcaggggca cttctatgtc gacgagacga ccggcaaggt gcacgcctcg
     8821 acctcggcct atgagcggcc gcagccgcat gcctgcttca tccagtcgat ccaggacgac
     8881 ctcgtcaacg agggcgggat catggatctc tgggtgcgcg aggccaggct cttcaagtac
     8941 ggctccggca ccggcaccaa tttctcgaac ctgcgcggcg agggcgagcg gctctcgggc
     9001 ggcggccgct cctccggcct gatgagcttc ctcaagatcg gcgaccgggc ggccggggcg
     9061 atcaagtcgg gcggcaccac gcgccgggcg gcgaagatgg tgatcgtcga tgtcgaccat
     9121 ccggacatcg aggccttcat cgactggaag gtcatcgagg agcagaaggt ggcggccctg
     9181 gtcgcgggct cgcggctgct gcagcgccat gccaacgcga tcatggaggc ctgccacggt
     9241 gcggccgacg tgccgcagga caagcgctgc gacccgctcg ccaacccgtt cctcaagcgc
     9301 gccatccgcg atgcgcgccg ggcgctcctg cccgagggct cgatccagca ggccattctt
     9361 ttggccgcgc agggctatac cgcggtcgag gtgccggtct acgacgccga ctgggacagc
     9421 caggcctatc tgaccgtggc cgggcagaac tcgaacaact cggtccggat cagcgacgcc
     9481 ttctaccagg cggtgcagca ggaccgcgaa tggccgctca ccgcgcgcat ctcgggcaag
     9541 gtggcgaaga cggtccgcgc caaggcgctc tgggacaagc tctgcttcgc cgcctgggcc
     9601 agcgccgacc ccggcgtgca gtacgacacc accatcaacg actggcacac ctgcccggcc
     9661 gacggccgca tcaatgcatc gaacccgtgc tccgagtaca tgttcctcga cgacacggcc
     9721 tgcaatctcg cgtcgttgaa cctgatgcag ttcagggcag ccgatggcag cttcctcgtc
     9781 gacgatttcg agcatgcggt ccggctctgg acgctggtgc tcgagatctc ggtgatgatg
     9841 gcccagttcc cggcggcccg gatcgccgag ctgagctacc ggttccgcac gctcggcctc
     9901 ggctacgcca atctcggcgg gctcctgatg gcgtccggca ttccctatga cagcgacgag
     9961 gggcgcgcct attgcggcgc catcaccgcg ctcatgaccg gtgtggccta tcgcgtctcg
    10021 gcggagctgg cggccgagct cggcgccttc ccgggctacg cgccgaaccg cgagtcgacg
    10081 ctgcgcgtgc tcaggaacca tcgccgcgcc gcgcacggcg aggcccgagg ctacgaggac
    10141 ctggccaccc tgcccgtccc gctcgacgcc ggccactgcc cgtccgagcc tctggccctg
    10201 gccgcgcgcc gggcctggga cgaggccgtc gccatggccg agcagcatgg cgtccgcaac
    10261 gcccaggtct cggtgatcgc ccccacgggc acgatcggcc tcgtcatgga ctgcgacacc
    10321 accggcatcg agcctgactt cgcgctcgtc aagttcaaga agctggcggg cggcgggttc
    10381 ttcaagatca tcaaccgcat ggtgccgcag gcgctggcca cgctcggcta cagcaaagcg
    10441 caggtcgagg cgatcacccg ctacgccgtg ggccacggca cgctgcagca cagccccggc
    10501 atcgaccacg cggcactgcg cgccaagggc ttcaccgacg aggtgctgaa gaagctcgag
    10561 gcgggcctgc cgaccacctt cgacatccgc tacgccttca accgcttctc gctcggcgac
    10621 gccttctgcc gggacgtgct cggcttcacc gacgagcagc tcggcgacgt gcgcttcgac
    10681 atgctgcagt cgctcggctt cagccgggac gcgatcgagc aggccaacgc cttctgctgc
    10741 ggcaccatga cgatcgaggg cgcgccggag ctgcggcccg agcatctggc ggtcttcgac
    10801 tgcgccaatc cctgcgggcg gatcggcagg cgctatctct cggcctcgag ccacatccgc
    10861 atgatggcgg cggcccagcc cttcatctcg ggggcgatct ccaagaccat caacatgccg
    10921 gcccgggcca cggtcgagga ttgccgcgag gcctatctcg agggctggcg gctcggcgtg
    10981 aaggcgctcg ccctctaccg cgacggctcc aagctgagcc agccgctctc ctcgatgctg
    11041 gccgacgtca tcgaggacga ggccgaggac gactcggccg cgcgctcgca ggtcgagcgc
    11101 atcgtcgagg tgacggagcg ggtggtcgag cgcttcgtca acggccgcag gaagctgccg
    11161 tccaggcgca agggctacac gcagaaggcc atcgtcggcg gccacaagct ctacttgcgg
    11221 accggcgagt acgcggacgg ggcgctcggc gagatcttcg tcgacatgca caaggaaggt
    11281 gccgccttcc gctcgctgat gaacagcttc gccatctcgg tctcgatcgg cctgcaatac
    11341 ggcgtgccgc tcgaggagtt cgtcgaggcc ttctcgttca cccgcttcga tccctccggc
    11401 gtcgtccagg gcaacgacac gatcaagatg gcgacctcgg tgctcgacta catcttccgc
    11461 gagctggcca tctcctatct ggagcgcacc gacctcgcca atgtcgatcc ggccgaattg
    11521 cgccacgacg ccatcggcgc cggcaccaag gagggcgaca tgggcgagaa cgtggcgcag
    11581 atcgcgtccg ccggcttcgt gcgcggcaat ctccgcctgc tcgccggcgg cggtgcggtg
    11641 atggagcccg cggcatcggg cggcggctcg cgccaggccg ggggcagcgc cgccgcggga
    11701 ttaaccacac tttcacgaga cagccgccgc cagcaggcgg aaatcgcccg ccttcagggc
    11761 tacgagggcg acccctgctc ggcttgcggc aacttcacgc tggtgcgcaa cggcacctgc
    11821 ctgaaatgcg acacctgcgg cagcacgact ggctgcagct aggtccgcat ggcgtacccg
    11881 tgggcagcgg gacgtctctt tccttgtatt ctcctcaact acggccccgg gttcgcccgg
    11941 ggccgctctt ctttggcgcc ggcgcccggg ctttcctgtc catccgcgac catgctcgtc
    12001 ctgatgcctg tcggccttcg tcttcggcaa gacggtcttt cgatgcgaaa ggtgatggcg
    12061 gattcgcgcg acacgcgagt cgaacggagt tggcgccggc ccgcccggac gccgccgcag
    12121 gccgtggcgg gcgggccttg gcccttgcgc cacagtctcc cccgacgcgc acccggcgag
    12181 cggcctttcc ggttgacgtc aactgtcagt tgcccgacat tttcaggcgc gactggcgct
    12241 gtcacgaggg cggaccgaga aacggaaagg gcgaagaagg cgagacaatg ggctggcgtc
    12301 cccgactttt gcacagatcc gacgaggtcc gaccgttcat cgagaagcag ctcgcggccg
    12361 ggaccgtcat cttctgggcc ggcgacccga gcgacgccgt cgccttgatc atcgatggcg
    12421 aggtcgaggt gctggcggag caggtcaacg gcctcgtgcg catcggcaag cacggcaatg
    12481 gcgcctatgt cggcgacaag gacgtcctgg ccgagcgccc gcggcgcttc acctttcgca
    12541 cgacccgccc gacccgcgtc gagatgctgt ccggccacct gttcatggag cggatgcggg
    12601 ccgccagggc cgcagccgag ccgcgcgccg aggcgctggt ccgcctcctg cccgcctcca
    12661 ccctcagcgc cggcctgctg ccggaggagg ggctctgcct caccagcttc ccgttcagcg
    12721 tcggccgcga gggcagcgac gggaaagagg acgcggcgag cgacgcacag gcccgcctgg
    12781 tcatcgccga gagccccccc tatcgcctgt cgcgtcgaca cttcaccatc ctgcgcgagg
    12841 gcgacatcat ccgggtggtc gacccgggca gtcgcctcgg caccctggtc gatggccagc
    12901 gggtcggcct cgagcccgtg acgcttgccg ccggcagcac gcacgagatc atcgcgggcg
    12961 gggcgagctc gccctaccgc ttccagcttg cgatcgactg acacgacgcc gagaatgcat
    13021 gcggcggccc gttcgttgcg gtcagaggag gatttcgatg tcggtcgttc acgagctgtc
    13081 gcggcggacg ttgctgctcg gcgcctgcct ctcctgggcg ctggccgcca cgccggccgc
    13141 cgaggccgcg accggggcgg agatcgacgc caaggtcgat ctcgccctgg cccagctcct
    13201 gcgcgaatcc gacaccgccc gggcgctcgc cgacaaggcg gtcgcgatcc tggtctttcc
    13261 cgacatcgtc aagggcggct tcggcatcgg cgggcagttc ggcgagggtg cgctgcgccg
    13321 caacggcaag accgagggct actacaacat cgccgccgcc tcgttcgggc tgcagatcgg
    13381 cgcccagtcc ttcagccagg cgatcttctt catgaccgag gaagccctgg cttatctcga
    13441 cagctccaag ggcttcgaga tcggcgccga tgccaatgtg gcgctggcca acgaggggct
    13501 gggctacgac gtgaccagca gcaccgtgca ggacccgatc atcgccttcg tcttcggcca
    13561 gaagggcctc atggccggcc tcacgatcga gggttcgaag atcacgaagc tcgccaggta
    13621 gccggcccgg ccagggcgac ggccacggag ccgcgcctgt cgctggtcac gctcgccgac
    13681 gacggctccc tgcagcttcc ctgagaccgt tggcggctgg cctcgggctc agccgtctcg
    13741 cttcacggcg ctctcgtgcc atttgcgcag caggagatgg ctgagcagcg tcagggcgag
    13801 gaagatcagg atgccggcca tggagatcag gaagagggcg gcgaacatgc gcgggatgtt
    13861 cagccggtag ctggcctcga ggatccggta ggcgaggccg gagccggtgc cggccgtgcc
    13921 ggcggcgaac tcggcgacga tggcgccgat cagcgccagc ccgcccgcga tccgcagccc
    13981 gccgaggaag tagggcatgg ccgagggcag gcgcaggaag cgcagcatct gccagcggct
    14041 cgccttgtag aggcggaaga ggtcgacgag gttgtggtcg gcgctgttca gcccgagcgt
    14101 ggtgttggac aggatgggga agaacgcgac gatccaggcg cagatcagaa gggcgaggtg
    14161 cgtgttctcg acatagatca ggatcagcgg ggcgatggcg atgatcggcg tcacctggag
    14221 gatcacggcg aaggggaaga gcgagagctc gatccagcgg gacagcgtga agagcacggc
    14281 gagcccgacc ccgccgacga ccgccacggc cagggcggag aaggtgaccc gcagcgtgac
    14341 caggagcgac gggtagagcg tgtgccagtc ggcgaccagg gtgcgggcca cgaggacggg
    14401 gcccggcagg atgtagtgcg gcgtgtcgtg ccagcggaca ccgagctccc acaggcccag
    14461 gacgacgagg acgaggccga gcggcatggc ccagcgcagc acccgctcga ggcgccgtgg
    14521 caaccgggac ggccggtcgc ccgccgccat cagcccacgg cctgctgcag ggccgccgag
    14581 gcctgcctgc aatagtggag atagctctcg gacaggcgga acgcatcgtc gcgcggatag
    14641 ggctcggcga tctcgaaccc ggcgacgatc cgccccgggc gcgccgacat gacgacgacc
    14701 ctggatgcca ggaagaccgc ctcgtagacg ctgtgggtga cgaagacgac ggtccagccc
    14761 tgcccctgcc agaggtcgag cagatcgtgg ttcagccggt gccgggtgat ctcgtcgacc
    14821 gcggcgaagg gctcgtccat gagcagcagc ctcggccggg tcaccagcgc ccgggcgatg
    14881 gacacgcgca tcctcatgcc gcccgagagc tcgcgcgggt aggccccggc gaagtcgtcg
    14941 aggccgacca gggcgagcgc ctctctgacc gcctcgccgg cccgccgccg cccgcgcccc
    15001 tgcagccgga gcggcaggtg gacattgtcg aagaccgtcg cccagggcat cagcgtcggc
    15061 tcctggaaga cgaagccgat atcccccgca ctgccgccgt tcggccaggc cagccggccg
    15121 gcgctcgcct cgccgagccc ggcgatgagg cgaagcgccg tgctcttgcc gcagccggag
    15181 gggcccagca tgcagaggag ctcgtggcgg ccgacatcca gatcgaggcc atccagcgcg
    15241 acggtgccgt tcgggaaggt cttcgagacg ccgcgcagct cgaggagagc atccgccgtg
    15301 gcaccgctcg gggtatccgc cctcacccgc cggtgagctc ccgcccgagc gccagcccga
    15361 cctgccggtt gacgaaggcg agcgtgtagg cacggctgag gtccaggtcg gccggataga
    15421 ggccgaccgc cgccaggctg gcgaggaagc tccgccagcg cgcatcggtc atggcgccga
    15481 tcccgagggt gagcgcatcg cccgattcga cgatgccacg ggccgtcatc tgctcgatcg
    15541 cgtaggcgat ctgcgcgtcg gtcatgtcgg ggttggcggc cttgatcagc gcgttgccgg
    15601 gcgtggggtc gccgtgcagg tagctcaccc agccgaggat cgaggcatcg acgaagcgct
    15661 gcacgaggtc cgggttctcc tcgaccagcc gccagctcgt ctcgatcatg ctggcatagc
    15721 tgtcatagcc gaagtcggcg agcaggagca ccctgggcgc gaagccgccc tcgcgggcga
    15781 cggcgaaggg ctcggcggtg agatagccct gctgggcgac ccccggatcg gcgatgaact
    15841 gcgccgcatt gaaggtgtag ggacggagct ggccgtcctc cagcccgtag gtgatcttga
    15901 gccagtccca gaaggtctgc cggccggcgg tggagatcag gatcggcatg tgcgcgaggt
    15961 cggccggggt ctcgatcccg cgatcggggt gggccatcag gaccagcggc gacttctgga
    16021 agatcgcggc caccgtcacc atcggcacgt cggccgtgag gtagttgaag gcgttggtca
    16081 tggaccccgc caggcagaaa tcgatccggc cggcggccag gagctgcgga tgattgacct
    16141 gcggcccgcc cggccggatg gtcacctcga ggccgtgcgc ctcgtagagg ccggtggcga
    16201 gcgcctgata gaagccgcca tgctcggcct gcgccttcca gttggtgccc agggtgacgg
    16261 cgtccagggc caaggccggg gcggcgagca gtgccgccaa ggagaacgac gaaaggagca
    16321 gggtcacggg gcggaacatg gacggttcca ggcgcagcgg cgtcgacccg cttcataggc
    16381 catcggcggc gtcgaaagtc gacgcgcgca accgccgcat ggcacgagcc ggaaaggccg
    16441 gccacggcaa ggtggcaaag gctgatgggg cgagcgaccg gacttgaacc ggcggcctcc
    16501 agggccacaa cctggcgctc taaccgactg agctacgccc gccatcagat ggcggcgtat
    16561 gtggcgcaaa ggccgggagg cgtcaagcat ccgccgctcc ggcaggtttg catgtatttt
    16621 gggcagcttg cctgttattt gatctttcgg cgcctgcggg tcgaccgccc ccgcacggcg
    16681 aagcgcattc ccaccggctg gcacgctccg tgcatatctg ttggcaccga tacgagatgc
    16741 aggtcgcgag cctgcccgcc ggtgcccgtg ccgcgcgatc gtcaccggtc ggggcggatg
    16801 gagggcggcg gtcatggctc gcagcaaggc ggaaggagat ccgagggcat gaagaagatc
    16861 gaggcgatca tcaagccgtt caagctcgat gaggtgaagg aggcgctgca ggaggtcggc
    16921 ctcaagggca tgaccgtgac cgaggtcaag ggcttcggcc gccagaaggg tcacaccgag
    16981 ctctatcggg gtgccgagta tgtcgtcgac ttcctgccga aggtgaagat cgagctggtg
    17041 gtcgacgatg cgatcgtcga ccgggcggtc gaggcgatcc agcaatcggc caggaccgac
    17101 cgcatcggcg acggcaagat cttcgtcctg ccggtcgagg atgcggtgcg catccgcacc
    17161 ggcgagcgcg gcgccgacgc cgtctgagcc acgagggggg gccggcgtcg gcgccggtca
    17221 tcgagcgtat gtggccgccg gctcgccggc cggccgccaa ccggggctcc gggccaggaa
    17281 caggcccggg aatgtgcaac aagaggttcg atcatgacac tgggatgcaa gtcggctgcg
    17341 gacgtgatga agtacgtcaa ggacaacggc gtggagatga tcgatctccg gctgatcgac
    17401 gtgcccggga tctggcagca tatctcctat ccggcggcga tcttcgacga gggcaacatc
    17461 gaggccggcc tcggcttcga cggctcgtcg gtgcgcggct tcgcgcagat ccaggcttcc
    17521 gacatgctgc tgcgcccgga tgtcaccacg gccttcctcg accccttcac caagcacaag
    17581 acgctggtga tgatctgcgc ggtcgccgat ccggtcaccg gcgagctcta cagccgcgac
    17641 ccgcgcggca tcgccatcaa ggccgaggag tatctcaagt ccaccggcat cggcgacacg
    17701 gcctatttcg gccccgaggc cgagttcttc gtcttcagcg acgtgcgcat gggccagggg
    17761 gtcaattacg gcacctacca ggtcgacagc cccgagggtc actggaacac gatgaccgac
    17821 gagggcccca acctcggcta caaggtcccc cccaagggcg gctacttccc ctgcgcgccg
    17881 accgactcgc tcaatgacct gcgggccgag atggcgctgg tgatgcaatc gatcggcctc
    17941 accgtcgaat gccaccacca cgaggtggcg accgccgggc agtgcgagat cgacatgagg
    18001 ttcgattcgc tcctggccat ggccgaccac cagatgaagt acaagtacgt ggtcaagaac
    18061 gtcgccaggg cggccggcat gaccgcgacc ttcatgccga agccgctcta tggcgacaac
    18121 ggctccggca tgcactgcca ccagtcgatc tggaacggct cgaccccgct cttcgccgac
    18181 gaggccggct atgccgggct ctcggacatg gcccgccatt acatcggcgg gctcctccgc
    18241 cacgccccct cgatcctcgc cttcgccgcg cccacgacca actcctatcg ccgcctggtg
    18301 ccgggctacg aggcgccggt caacctcgcc tggtcgctca ggaaccgctc ggccgcctgc
    18361 cgcatcccga tgtactccaa cagcccgaag gcgaagcggg tcgagttccg ctgccccgac
    18421 ccctcggcca acccgtatct cgccttcgcg gcgatgctca tggccggcat cgacggcatc
    18481 aagaacaaga tcgacccggg cgcgcccatc gacaagaaca tctacgacct gccgcccgag
    18541 gaagcggccg agatcccgaa ggtcccgggc cagctcgccg acgc
//
LOCUS       DAOTFI010000082        18540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3600_length_18540_cov_7.376846, whole genome shotgun sequence.
ACCESSION   DAOTFI010000082 DAOTFI010000000
VERSION     DAOTFI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18540
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_18540_cov_7.376846"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="P5558_10185"
     CDS             complement(<1..1358)
                     /locus_tag="P5558_10185"
                     /inference="COORDINATES: protein motif:HMM:NF016433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HRY24720.1"
                     /translation="MAKAAVTTKPKDNDSVGASLETLMASAPALRRLVTQAKARGTVT
                     LDQLNAALPTETNPEQIDDLISGLESRGIGVVRDEAGDGNGDGDTAAANENEKPEAEG
                     EEESESETATQSIGDEDLGRTDDPVRMYLREMGTIELLSREGEIEIAKRIESGRNMVL
                     EALCESPLTMRAVIGWRDAIRDGRVLLRDIIDLDATNEAGFNPEAEGKAAAEPAAAGD
                     PAAADAAGEGAEGEAKPEVAESEEEDFDDDTLSLSAMEDKLRDGVLESFDRIADAYAI
                     LRELQDERLAAVQAAESIDAEVDRRYEAARETIVELMKQVQLNNGRVEALVEQLFDMN
                     KLLQGLEGKLMRLAVGSRISREAFIAEYMGNELDPDWMSRVASLKGKGWADFISRNYE
                     AIEGLRADIGAIAEETGLKIAEFRRIVAKVQKGERDAGRAKKEMVEANLRLVISIAKK
                     YT"
     gene            complement(1611..3467)
                     /gene="dnaG"
                     /locus_tag="P5558_10190"
     CDS             complement(1611..3467)
                     /gene="dnaG"
                     /locus_tag="P5558_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRY24721.1"
                     /translation="MSGRAYDGGRSNDGQLEAFKARLPLLEIVGRQVRLIRRGNKHVG
                     LCPFHQEKSPSFTVFPDQGNYHCFGCGAHGTAIDFVMATEGLGFTEALRRLADLTGIP
                     APLREPAAGPGRPPTEPLVAANETAARWFQGRLAAGEGRVARDYLAQRGVPPDLQHRF
                     ALGYAPDSRDGLVRALTAAGIPPAVQAEAGLVGLPEEGGSAYDRFRHRLMFPIRDQRG
                     RLVGFGGRALGEARAKYLNTSETPIFHKGSLLYGFDLAAKPARERREIFIVEGYLDVI
                     AMAGAGFENTTAPLGTAVSEEQLRLLWRHADEPYVCLDGDAAGLAAAMRMIRRALPIM
                     AGGQTLRFIVLPSGEDPDSLLRASGRDALEQLVRAAVPLSRMIWQVELAAAPAETPEQ
                     IAGLRHRLLEYVRLAGDRSLQDGLRSHFDGLVYELRRAVNGRRAPDGRQPGRGRGDAR
                     GAAPGRPGWGGVGAARLASSLASRARDANLELLGAFLEQPALLDSFGEELAAVPFDHP
                     DADTARRELLNWFAATDHLDAVDLRNHLTRYGFAVVVEQALQRFAVLTRTVDEEAGID
                     ELLGRMLAGLRLRAARRDEAEAFAGDIGGGDTDELAGRRFDRLLNDRTDDES"
     gene            complement(3501..3947)
                     /locus_tag="P5558_10195"
     CDS             complement(3501..3947)
                     /locus_tag="P5558_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRY24722.1"
                     /translation="MLRERLASALREAPDADTAATLRLVLAALKERDHCARESGAAEE
                     LDDGAIETMLQDMVMQRQDEILRCEECARLEEAEQEAAEIGVLKQFLPRRMSDSEVDA
                     AVDGMIDEVGAKRLKDTGKVIAALKERYNGEMDFARARRRVCARLA"
     gene            4267..5418
                     /gene="carA"
                     /locus_tag="P5558_10200"
     CDS             4267..5418
                     /gene="carA"
                     /locus_tag="P5558_10200"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRY24723.1"
                     /translation="MQAGATAPSAVLALEDGTILAGRGFGGTAVVTGELVFNTAMTGY
                     QEILTDPSYAGQIVTFTFPHIGNVGTNDEDREALTPVVRGLVTRARVTRPSSWRDRLD
                     LVGWLERQGIPGLAGVDTRRVTAKLRDRGALRACLAHDPAGIADPGALVERARGWPGI
                     VGMDLASEVSARQRYGWQEAAWSPEGGYGASDGSGPHVVVIDYGVKHNILRSLAARNC
                     RVTVVPGTASADEVLALEPDGVVLGNGPGDPAATGIHAVPEIRRLLATGKPLFGICLG
                     HQMLGLALGARTEKMSFGHHGANHPVQDLGTGKVEITSQNHGFAIADGDLPDDVEVTH
                     RSLFDATIQGIRIKDRPVYAVQYHPEASPGPQDSQYLFDRFLDDVRRQN"
     gene            5425..8676
                     /gene="carB"
                     /locus_tag="P5558_10205"
     CDS             5425..8676
                     /gene="carB"
                     /locus_tag="P5558_10205"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRY24724.1"
                     /translation="MPKRTDIRSILLIGAGPIVIGQACEFDYSGTQALKALREEGYRV
                     ILVNSNPATIMTDPELADATYIEPITPAIVAKIIEKERPDALLPTMGGQTALNVARAL
                     DEDGTLARFGCELIGASRAAIDKAEDRELFRRAMLKIGLAMPMSIIAHSLAEAREAVE
                     ATGLPAIIRPSFTLAGTGGGIAYNREEFEQIVASGLEASPITQVLVEESVLGWKEYEM
                     EVVRDRKDNCIIVCSIENVDPMGIHTGDSMTVAPALTLTDKEYQLMRTASIAVLREVG
                     VDTGGSNVQFAIDPRTGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IQNDITGATPASFEPTIDYVVTKIPRFAFEKFPETEPLLTTSMKSVGEVMAIGRTFKA
                     SLQKALRSLENGLTGLDELPIEAGADPHEFLQRELARPTPDRFRVAADAIRAGLELAV
                     IQRITAFDPWFLEQLEELVATEARVRAEGLPKDAAGWLELKADGFGDARLAKLTGTAE
                     AEVRRLRHAAGVHPVFKRVDTCAAEFQARTPYMYSAYEEGLWDGSPAECESRPSDRQK
                     VLILGSGPNRIGQGIEFDYCCVHAAFGLKEAGFETIMVNCNPETVSTDPDTSDRLYFE
                     PLTAENVIEIARVEASRGELLGAIVQLGGQTPLKLAAALQEAGIAILGTSPDAIDLAE
                     DRDRFQRLLNELGLRQPANAICRNEQEAVERAAELGVPLVIRPSYVLGGRAMRIVHSL
                     DDVRQFAAEAAVAAEHGTILLDRYLQDAIEVDVDVLADGETVVVTGIMEHIEEAGIHS
                     GDSACSLPPYSIGAAVLAEIRSSAEALARALDVKGLMNVQMAVQGETVFIIEVNPRAS
                     RTVPFVAKAIGTPIAKIAARIMAGERLADFRVNGGPVGHVAVKEAVFPFARFPGVDLL
                     LGPEMKSTGEVMGLDRDFGTAFAKSQLAAGVALPSGGRVFVSVRDEDKEKAVRLASDL
                     AALGFTLVATHGTAAALEAAGLVVETVNKVHEGRPHIVDSIKDREIAILINTSGGQQA
                     IRDSFSLRRAALMAKLPYYTTVSGARALVEGLKRLQQGELTVAPLQSYVAHLN"
     gene            8752..9246
                     /gene="greA"
                     /locus_tag="P5558_10210"
     CDS             8752..9246
                     /gene="greA"
                     /locus_tag="P5558_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRY24725.1"
                     /translation="MVTKIPMTPEGHTRLGAELKRLKNVERPAVIKAIAEAREHGDLS
                     ENAEYHAARERQGFIEGRVMELEDKLARAEVIDVAAQSGSKVVFGAKVKLVDEETEEK
                     ILYQIVGPEEAEIQQGLLSIASPLAQALLGKEAGDSVEVTTPRGVRYFEILSVGFGEV
                     EAAQ"
     gene            9262..10206
                     /gene="trxB"
                     /locus_tag="P5558_10215"
     CDS             9262..10206
                     /gene="trxB"
                     /locus_tag="P5558_10215"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRY24726.1"
                     /translation="MGSERHTRLLIIGSGPAGYTAAIYAARANLQPILVQGLQPGGQL
                     SITTDVENYPGFAEAIQGPWLMEQMAAQATNCGAELVFDIITEVNLDGPPFRAKGDSG
                     DVIVADSLIIATGAVARWLGLPSEQHFQGHGVSACATCDGFFFRGKKVAVVGGGNTAA
                     EEALYLAQMCEKVTLVHRRDALRAEKILQDRVFAHPKIEILWDHVVEEVLGSATPKSV
                     TGLRVRHVATGATAELAVEGVFIAIGHDPATELFKGRLEMDGEGYLVTAPDSTRTGIP
                     GVFAAGDVQDKIYRQAVTAAGTGCMAALEAERYLASRE"
     gene            10272..11171
                     /locus_tag="P5558_10220"
     CDS             10272..11171
                     /locus_tag="P5558_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY24727.1"
                     /translation="MDWDRVRIFHAVAGAGSFTRAADRLGLSQSAISRQIGALEATIG
                     TPLFHRHARGLILTEQGDVLLEAANEVAKRLAAVETTLGEVRDEAAGHLRVNTTVGLG
                     TVWLTSHLFDFRELYPDITLSLVVTDSDVDLAMRQADVAIRLHRPTQPDLVQRRLMTV
                     HTHIYGGHTYLERHGIPETVGDLDAHALIAYGDQWQPPVPSLNWILAAGRDEDEVHGR
                     APALTVNNVYGMLRACECGLGLASLPDYLGGTNARLRRILPTLEGPSFTAYFVYPEEL
                     RASKRVSVFRDFLLRKVAEQPVW"
     gene            complement(11546..12505)
                     /gene="msrP"
                     /locus_tag="P5558_10225"
     CDS             complement(11546..12505)
                     /gene="msrP"
                     /locus_tag="P5558_10225"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HRY24728.1"
                     /translation="MLTRTRKGWELPESAATPKSVYVNRRQLLAAGGAGAMLAALPRG
                     LRAAEEAPIAAELYPAPRNETYVLDRELTDPELATTYNNFYEFGSHKQISLAAQALRL
                     RPWAVTIDGLVEDEIVLDAEELIRRMPVEERLYRHRCVEAWSMAVPWTGFPMKALVEL
                     ARPLSAAKYVVMTTFEDSDMAPGQKQFWYPWPYVEGLTIEEATNDLTLIGTGIYGEPM
                     PRQNGAPLRLVVPWKYGFKSVKSLVRFTFSDTRPVSFWQALVPEEYGFWANVNPEVPH
                     PRWTQAEERVLGTNEMVPTLPFNGYGEFVAQLYAAMQGEQLYM"
     gene            complement(12606..14471)
                     /locus_tag="P5558_10230"
     CDS             complement(12606..14471)
                     /locus_tag="P5558_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24729.1"
                     /translation="MRSLVSFMFRRFETLIDPFPEAEPTRPPDRLIPFLWHFTRPVAP
                     WIAIMAVVTATLSTLEIVFLGFLGSLVDTLAGADRATFFADHGPTLIGMVLLIVVVFP
                     VFVLLQNLLLHQTIFGNYPMLARWLAHRYALGQSLSFFQDEFAGRVSQKVMQTALAIR
                     ETVITLTEVMVYVVVYFTGAMVLLGQADLWLMLPLVLWLAGYLGILRYFVPRLRRVSM
                     AQADARAEMTGRVVDSYTNIQTVKLFAHSRREQVYAREAMDGFMVTVHQQMRLASMLT
                     FSLHCLNSLLLAGIGGTAVLAWHQELISLGAIAVAVSVVMRIRAMSQWILWEVGKLFE
                     NIGTTQDGMTTLARPHTIVDRPGAPPLRVPQGEIRFDNVHFNYGRPGGIIEGLTLHIR
                     PGEKVGLVGRSGAGKSTLVNLLLRFYDPESGRILIDGQDIATVQQESLRQQIGMVTQD
                     TSLLHRSIRDNIRYGSPDATDEEIERAAERAHADTFIQDLTDLKGRHGLDAMVGERGV
                     KLSGGQRQRIAIARVLLKNAPILILDEATSALDSEVEAAIQEQLQSLMAGKTVIAIAH
                     RLSTIAMLDRLVVMDKGRIVESGSHAELLRRNGLYAGLWHRQSGGMLGLLDTAAE"
     gene            14812..16401
                     /locus_tag="P5558_10235"
     CDS             14812..16401
                     /locus_tag="P5558_10235"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HRY24730.1"
                     /translation="MDRALLDAVNRRSMLRIAAGCGVLAASAGLARTAAVGTADAAAA
                     EPDGPFTFETVIAQARRLAGEEHEAAKIELKGPFADLDYDHYRAIRFRPERRFWQDEK
                     RGFQLDLLPPGFYYQDRIDLALVVDGVVHPLEFDPGLFHYHPDYFPFPDGAAPGDAPR
                     DLAYSGFRVRFPINRPDVMDEFLVFQGASYFRAVARDQLYGLSARGLALGTGSPEGEE
                     FPLFRKFWIHEPQVAATSLTIQALLDSPSVTGAYEFIVRPGAETVMDVRSILFPRADL
                     TAAGIAPLTSMFFFGPKSRARIDDYRDAVHDSSGLQMVTGAGERIWRPLDNPLTLQFS
                     AFSDHDPQGFGLTQRERAFTHYEDSEARYERRPSAWVAPSNAWGRGSVVLVEIPTDTE
                     FNDNVVAFWRPAEPLTRGSEHPFDYRLLWSGEPPDETPLARVVATRGGVKVAVPEDRS
                     FIVDFDLGELPFEGLEPRVTASAGDITHVALVRLPAESRARAIITFHPGDAGLAELRL
                     SLVDAEGRTASETWLARWTPR"
     gene            16398..18497
                     /gene="mdoH"
                     /locus_tag="P5558_10240"
     CDS             16398..18497
                     /gene="mdoH"
                     /locus_tag="P5558_10240"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRY24731.1"
                     /translation="MTRIDQAPVPPPAPMAMPAQDLRRVPERRFRSQGWRVHVARLVA
                     FGGALALTVLGAQQMGRVFDPAHVSALQTTLSVLFTLTFGWIAFSAAAAVAGLLAVRP
                     QRPAAAKAGEGPLGRTAIVMPVYNEDPVATAGALAAMGQGLAELGHGEDFEIFILSDT
                     RDATHWARETAVFAALRQRLEGRMAVWYRRRARNTARKAGNLQDFIERWGGRYDYLLS
                     LDADSLMAPETIVEMARRMAAEPRLGLLQTVPMLAGGETLYARLQQFAGRLHGPAIAR
                     GTAAWQGDDGNYWGHNAMLRTAAFAQSAGLPELRGRAPFGGHVLSHDFVEAALLRRAG
                     WIVRMDPDLDGSFEGAPPSLADAAARDRRWAQGNLQHLKVIGARGLRWPSRVHFLIGV
                     GSYLASPLWLAMILVGLVLTAQAIFIQPEYFPNTYQLFPEWPRFDALRMRWLFILSIG
                     LLLLPKLFGLARALAHRPTRERFGGARRLLPGALAEIVLSALYAPVMMLMQTRQLFEI
                     LAGRDSGWSTQARNGSRITWRDALGRHWRHALAGLVVSAGLLYAAPTLLPWLAPVLVG
                     LVLAPWLCRVSGDPAVGARFARLGLLVTPEETRPHRIFAAARTEAAHLAPAGVVGLGD
                     LARLEGARAAHVASLTEADRAELDPLAVITARAKIQAAATTEQALDWLTEAERRALLA
                     VPDLLDALARQPVLLAA"
BASE COUNT         2804 a         6456 c         6426 g         2854 t
ORIGIN      
        1 ttggtgtatt tcttggcgat ggagatgacg aggcggaggt tggcctcgac catctccttc
       61 ttggcgcgac ccgcgtcgcg ctcgcccttc tgcaccttgg cgacgatgcg gcggaactcg
      121 gcgatcttga gcccggtctc ctcggcgatg gcgccgatat ccgcgcgcag gccctcgatg
      181 gcctcgtagt tgcgcgagat gaaatcggcc cagcccttgc ccttgaggct ggcgacgcgg
      241 ctcatccagt ccgggtcgag ctcgttgccc atgtactcgg cgatgaacgc ctcgcggctg
      301 atccggctgc ccacggcgag gcgcatcagc ttgccctcga ggccctgcag cagcttgttc
      361 atgtcgaaga gctgctcgac cagcgcctcg acccgaccgt tgttgagctg cacctgcttc
      421 atcagctcga cgatggtctc gcgcgccgcc tcgtagcgac ggtcgacctc ggcgtcgatg
      481 gattccgccg cctgcacggc ggcgagccgc tcgtcctgca gctcgcgcag gatggcgtag
      541 gcgtcggcga tccgatcgaa gctctcgagc acgccgtcgc ggagcttgtc ctccatcgcc
      601 gagagcgaca gggtgtcgtc gtcgaagtcc tcctcctcgc tctcggccac ctcgggcttg
      661 gcctcgcctt cggccccctc cccggcggcg tcggccgctg ccgggtcgcc ggccgccgcc
      721 ggctcggccg ccgccttgcc ctcggcctcc gggttgaagc cggcctcgtt ggtggcatcg
      781 agatcgatga tgtcgcgcag gagcacgcgg ccgtcgcgga tggcgtcgcg ccagccgatc
      841 acggctcgca tcgtcaaggg gctctcgcag agcgcctcga ggaccatgtt gcggccggac
      901 tcgatgcgct tggcgatctc gatctcgccc tcgcgggaga gcagctcgat cgtgcccatc
      961 tcgcgcagat acatgcgcac gggatcgtcg gtgcggccga gatcctcgtc gccgatggac
     1021 tgcgtcgcgg tctcgctctc gctttcctcc tcgccttcgg cttccggctt ctcgttctcg
     1081 ttggcggcgg ccgtgtcgcc atcgccgttg ccgtcgcccg cctcgtcgcg gacgacgccg
     1141 atgccgcgcg attcgagccc ggagatcaga tcgtcgatct gctcggggtt ggtctcggtc
     1201 ggcagcgccg cgttgagctg gtcgagcgtg acggtgcccc gcgccttggc ctgggtcacc
     1261 aggcgccgca gcgccggtgc cgacgccatc agtgtctcca gggaggcacc gacgctgtca
     1321 ttgtccttcg gcttggtggt caccgcagct ttcgccatgc accctcgctc cgcctgtcaa
     1381 attccgccgt gccggccggc tcgctgccgc gcccgccaca tccgctcgtc aacgcgcaat
     1441 cctcggggca cggcgaacgg gacggggcag cagcgtgcca caccgtcccc cacctcctgc
     1501 caggcgcccc gaaaaaactc agccccgtgt cgccatttcg tcactaccct cgccgctcga
     1561 tcgcgggctt tcggccccgt cgatcggctc cggtggccgg gccaccgctc tcagctctcg
     1621 tcgtccgtcc tatcgttcaa caggcgatcg aagcgtcgac ccgccagctc atccgtgtct
     1681 ccgccgccaa tatccccggc gaacgcctcg gcctcgtcgc gccgcgccgc ccgcaaacgc
     1741 aagcccgcca acatgcggcc gagcagctcg tcgatgccgg cctcctcgtc gaccgtccgc
     1801 gtcaggacgg cgaaacgctg gagcgcctgc tcgacaacca cagcaaaacc gtagcgggtc
     1861 agatggttgc gcaggtccac cgcgtcaagg tggtcggtgg cggcgaacca gttcagaagc
     1921 tcgcggcgtg ccgtatcggc atcagggtga tcgaacggca cggcggccag ctcctcgccg
     1981 aaggaatcga gcagcgccgg ctgctcgaga aaggcgccga gcagctcgag attggcatcc
     2041 ctggcgcggc tggcgagcga cgaggcgagc ctcgcagcac cgacgccgcc ccagcccggc
     2101 cggccgggtg cggcacccct ggcatcgccg cggccgcgcc cgggctggcg cccgtcgggc
     2161 gcccgccggc cgttgacggc ccggcgcagc tcgtagacga gaccgtcgaa gtgcgaccgc
     2221 aacccgtcct ggaggctgcg gtcgccggcg agccggacgt attcgaggag gcggtggcgg
     2281 agcccggcga tctgctccgg cgtctcggca ggcgcggcgg cgagctcgac ctgccagatc
     2341 atccgcgaca gcggcaccgc cgcccggacg agctgctcca gcgcgtcgcg gccgctggcc
     2401 cgcagcaggc tgtccgggtc ctcgcccgaa ggcagcacga tgaagcggag cgtctggccg
     2461 ccggccatga tcggcagggc acggcggatc atgcgcatgg cggcggcgag cccggcggca
     2521 tcgccgtcga ggcagacata gggctcgtcg gcatggcgcc agagcaggcg aagctgctcc
     2581 tcgctcacgg cagtgcccaa aggcgcggtg gtgttctcga agccggcccc ggccatggcg
     2641 atgacgtcga gatagccctc gacgatgaag atctcgcggc gttcgcgggc gggcttcgcc
     2701 gccagatcga agccgtagag caggctgccc ttgtggaaga tcggggtttc ggacgtattc
     2761 aggtacttgg cccgcgcctc gcccagggca cgcccgccga agccgaccag ccggccgcgc
     2821 tggtcgcgga tcgggaacat cagccgatgg cggaagcggt cataggccga gccgccctcc
     2881 tcgggcaggc cgacgaggcc ggcctcggcc tgcacggccg gcgggatgcc cgcggccgtc
     2941 agcgcccgga ccaggccgtc gcggctgtcc ggcgcgtagc ccagggcgaa gcggtgctgc
     3001 agatccggcg gcacgccgcg ctgggccagg tagtcccggg cgacccggcc ttcgcccgcg
     3061 gcgagccggc cctggaacca gcgcgccgcg gtctcgttgg cggcgaccag cggctcggtc
     3121 ggcggtcgcc cggggccggc ggccggctcg cgcagcggtg ccgggatgcc ggtgaggtcg
     3181 gcaaggcgcc gcaacgcctc ggtgaagccc agcccctccg tcgccatgac gaagtcgatg
     3241 gcggtgccgt gcgcgccgca gccgaagcag tggtagttgc cctggtccgg aaagacggtg
     3301 aacgagggcg acttctcctg gtggaacggg cacaagccga catgcttgtt gccccggcgg
     3361 atcaggcgga cctggcggcc gacgatctcg agcagcggca ggcgcgcctt gaacgcctcg
     3421 agctggccat cattcgaccg gcctccatca tacgcccgtc cgctcaagat cgcggccgcg
     3481 acgatgccgg gcgcgggcgc tcaggcgagc ctggcgcaga cccgtcgcct ggcgcgcgcg
     3541 aaatccatct cgccattgta gcgctccttc aaggccgcga tcaccttgcc ggtatccttg
     3601 agccgcttgg cgccgacctc gtcgatcatc ccgtcgaccg ctgcatcgac ctcgctgtcg
     3661 ctcatccgcc gcggcaggaa ctgcttgagg acgccgatct cggcggcctc ctgctcggcc
     3721 tcctcgagcc gcgcgcattc ctcgcagcgc aggatctcgt cctggcgctg catcaccatg
     3781 tcctggagca tggtctcgat ggccccgtcg tccagctcct cggcggcgcc ggattcgcgg
     3841 gcgcaatggt cgcgctcctt gagtgcggcc aggaccaggc gcagcgtcgc tgcggtatcg
     3901 gcatccggtg cttcccgcaa tgccgaagca agacgttctc tcaacacgac cacgaactca
     3961 cggtcatgat caacgacctc tcgaactggg gcggcggcat ccggggccgc cgctcgattg
     4021 ttggtttccg ccctctttcg accccggctc accgcctcac gcgatcaggg ccagggtatc
     4081 cgcccatcgt caggttgcct cgcttgacgg cactccgcac aatgctacaa accgccccgg
     4141 cgcggaccaa ccggccggac ccacagccac gccgggcagt ttcgaccaac caggcgccgc
     4201 ctttcggcat gcgacccttc gaagcccggc acggcggccc gactctctac ttagattggc
     4261 ccagcgatgc aagcaggtgc gaccgcaccc tccgccgtcc tggctctgga ggacggaacg
     4321 atcttggccg gccgcggctt cggcggcaca gccgtcgtca cgggcgagct ggtcttcaac
     4381 acggccatga ccggctacca ggagatcctc accgatccgt cctacgccgg gcagatcgtc
     4441 acgttcacgt tccctcatat aggcaatgtc ggcaccaatg acgaggaccg ggaggcgctg
     4501 accccggtgg tcaggggcct cgtgacccgt gccagggtga cgcggccctc gagctggcgc
     4561 gatcggctcg atctggtcgg ctggctcgag cgccagggca ttcccggcct cgccggcgtc
     4621 gacacgcggc gcgtcacggc gaagctgcgc gaccggggcg cgctccgcgc ctgcctcgcc
     4681 cacgatccgg ccggcatcgc cgacccgggc gccctggtcg agcgggcccg gggctggccc
     4741 ggaatcgtcg gcatggacct cgcgagcgaa gtgtcggccc ggcagcgcta tggctggcag
     4801 gaggcggcct ggagcccgga gggcggctat ggcgccagcg acggcagcgg tccgcatgtc
     4861 gtggtcatcg actacggcgt gaagcacaac atcctgcgct cgctcgccgc ccgcaactgc
     4921 cgggtgacgg tggtgccggg gactgcctcg gccgacgagg tgctggcgct cgagccggac
     4981 ggcgtggtgc tcggcaacgg gccgggcgac ccggcggcga ccggcatcca cgcggtgccg
     5041 gagatccggc ggctgctcgc caccggcaag ccgctcttcg gcatctgcct cggccaccag
     5101 atgctcggcc tggcgctggg ggcgcggacg gagaagatgt cgttcggcca ccacggcgcc
     5161 aaccatccgg tccaggatct gggcaccggc aaggtcgaga tcaccagcca gaaccacggc
     5221 ttcgccatcg ccgacggcga cctgccggac gatgtcgagg tgacccatcg ctcgctcttc
     5281 gatgcgacga tccagggcat ccgcatcaag gaccgtccgg tctacgccgt gcagtaccat
     5341 cccgaggcgt caccggggcc gcaggacagc cagtatctgt tcgaccgctt cctcgacgac
     5401 gtccgccggc agaattagag aaccatgccc aaacgcaccg acatccgctc gatcctgctg
     5461 atcggggccg gcccgatcgt catcggccag gcctgcgagt tcgattattc cggcacgcag
     5521 gccctgaagg cgctgcgcga ggagggctac cgggtcatcc tggtcaactc caacccggcg
     5581 acgatcatga ccgatccgga gctggcggac gcgacctata tcgagccgat cacgccggcc
     5641 atcgtggcca aaatcatcga aaaggagcgc cccgacgcgc ttctgccgac catgggcggg
     5701 cagacggcgc tcaatgtcgc ccgcgccctc gacgaggacg gcacgctggc ccgcttcggc
     5761 tgcgagctga tcggcgcctc gcgggcggcc atcgacaagg ccgaggatcg cgagctcttc
     5821 cgccgggcga tgctgaagat cggcctcgcc atgcccatga gcatcatcgc ccacagcctc
     5881 gccgaggccc gggaggcggt cgaggcgacc ggcctgccgg cgatcatccg cccgtccttc
     5941 acgctggccg gcaccggcgg cggcatcgcc tacaaccggg aggagttcga gcagatcgtg
     6001 gccagcgggc tcgaggcctc gcccatcacc caggtgctgg tcgaggaatc ggtcctgggg
     6061 tggaaagagt acgagatgga ggtggtgcgg gacaggaagg acaactgcat catcgtctgc
     6121 tcgatcgaga acgtggaccc catgggcatc cacaccggcg acagcatgac cgtggcgccg
     6181 gccctgacgc tgaccgacaa ggaataccag ctcatgcgga cggcctcgat cgccgtcctg
     6241 cgcgaggtcg gggtcgacac gggcggctcg aacgtgcagt tcgccatcga tccgaggacc
     6301 gggcggatgg tggtgatcga gatgaacccc agggtctcgc gctcctcggc cctggcgtcg
     6361 aaggcgacgg gctttccgat cgccaaggtc gcggccaagc tggccgtcgg ctacacgctc
     6421 gacgagatcc agaacgacat caccggcgcc acgccggcct cgttcgagcc gaccatcgac
     6481 tacgtggtca ccaagatccc gcgcttcgcc ttcgagaagt ttcccgagac cgagcctttg
     6541 ctcaccacct cgatgaagtc ggtgggcgag gtcatggcga ttggccggac cttcaaggcg
     6601 tcgctgcaga aggcgctgcg cagcctcgag aacggcctga ccgggctcga cgagctgccg
     6661 atcgaggccg gcgccgaccc gcacgagttc ctgcagcgcg agctcgcccg gccgaccccg
     6721 gaccggttcc gggtggcggc cgacgcgatc cgcgccggcc tcgagcttgc ggtcatccag
     6781 cgcatcaccg ccttcgaccc ctggttcctc gagcagctcg aggagctggt ggcgaccgag
     6841 gcgcgggtcc gtgccgaggg cctgccgaag gacgcggccg gctggctcga gctcaaggcc
     6901 gacggcttcg gcgatgcgcg gctggcgaag ctcaccggca cggccgaggc cgaggtgcgc
     6961 cggctccggc atgcggccgg ggtgcatccc gtcttcaagc gggtcgacac ctgtgcggcc
     7021 gagttccagg cgcgcacccc ctacatgtac agcgcctatg aggagggcct gtgggacggc
     7081 agcccggccg agtgcgagag ccggccgtcc gatcgccaga aggtgctgat cctcggcagc
     7141 ggcccgaacc ggatcggcca gggcatcgag ttcgactact gctgcgtgca tgccgccttc
     7201 ggcctcaagg aggccggctt cgagaccatc atggtcaact gcaacccgga gaccgtctcg
     7261 accgacccgg acacctccga ccggctgtat ttcgagccct tgacggccga gaacgtgatc
     7321 gagatcgccc gcgtcgaggc cagccggggc gagctgctgg gggccatcgt ccagctcggc
     7381 gggcagacgc cgttgaagct cgcggcggcg ctgcaggagg ccgggatcgc cattctcggc
     7441 acctcgcccg acgccatcga cctcgccgag gaccgtgacc gcttccagcg tctcctcaac
     7501 gagctcggcc tgcgccagcc ggccaacgcc atctgccgca acgagcagga ggcggtcgag
     7561 cgggccgccg agctcggcgt gccgctggtc atccggccgt cctacgtgct cggcggacgg
     7621 gcgatgcgca tcgtccactc gctcgacgac gtgcgccagt tcgcggccga ggcggcggtg
     7681 gcggccgagc acggcacgat cctgctcgac cgctacctgc aggacgcgat cgaggtggat
     7741 gtcgacgtgc tcgccgacgg cgagacggtg gtcgtcaccg gcatcatgga gcacatcgag
     7801 gaggccggca tccattcggg cgacagcgcc tgctccctgc cgccctactc catcggcgcg
     7861 gccgtgctcg ccgagatccg cagcagcgcg gaggcgctgg cccgcgccct cgacgtcaag
     7921 ggcctgatga acgtgcagat ggcggtgcag ggcgagaccg tgttcatcat cgaggtgaac
     7981 ccgagggcca gccgcacggt gccgttcgtc gccaaggcga tcggcacgcc gatcgcgaag
     8041 atcgcggccc ggatcatggc gggcgagcgg ctcgccgatt tccgggtcaa tggcggtcct
     8101 gtgggccatg tcgcggtcaa ggaggcggtc ttcccgttcg cccggttccc gggcgtcgac
     8161 ctcctgctcg gcccggagat gaagtcgacc ggcgaggtca tgggcctgga ccgtgatttc
     8221 ggcacggcct tcgccaagtc gcagctcgcc gccggggtgg ccctgccgtc cggcgggcgg
     8281 gtcttcgtct cggtccgcga cgaggacaag gagaaggccg tgcggctcgc cagcgatctc
     8341 gccgcgctcg gcttcaccct ggtcgcgacg cacggcaccg cggcggccct ggaggcggcc
     8401 gggctcgtcg tggagacggt caacaaggtg cacgaggggc ggccgcacat cgtcgattcg
     8461 atcaaggacc gggagatcgc gatcctgatc aacacctcgg gcgggcagca ggcgatcagg
     8521 gacagcttca gcctgcgtcg agcagccttg atggccaagc tgccctacta tacgacggta
     8581 tcgggggctc gcgccctggt tgaggggctg aagcgcctgc agcagggtga gctgaccgtt
     8641 gcgccattgc aatcctacgt ggcgcactta aactaaccag tcgacgacgt ccgtcgcacc
     8701 gttggatgcg gccacgtcga gatagccatt tctattggaa cgatagctgt catggttacg
     8761 aagatcccga tgacaccgga ggggcacacg cgactgggcg cggagctgaa gcgcctgaag
     8821 aatgtcgagc ggccggccgt tatcaaggcg atcgcggaag cgcgcgagca cggtgatctg
     8881 tcggagaacg ccgagtatca cgcggcacgc gagcgccagg gcttcatcga gggccgggtc
     8941 atggagctcg aggacaagct ggcgcgcgcc gaggtgatcg acgtcgcggc gcagtcgggc
     9001 agcaaggtgg tgttcggcgc caaggtgaag ctggtcgacg aggagaccga ggagaagatc
     9061 ctctaccaga tcgtcgggcc cgaggaggcg gagatccagc agggcctgct gtcgatcgcc
     9121 tcgccgctcg cccaggccct gctcggcaag gaggccggtg acagcgtcga ggtgacgaca
     9181 ccgcgagggg tgcgctattt cgagatcctg tcggtcgggt tcggcgaggt cgaggcggcg
     9241 cagtaggagc tcgtcctcgc catgggcagc gaacggcaca cccggcttct gataatcggc
     9301 tccggcccgg cgggctacac ggccgccatc tatgcggcgc gggccaatct gcagccgatc
     9361 ctggtgcagg ggctgcagcc gggcggccag ctctcgatca ccaccgacgt ggagaactac
     9421 ccgggcttcg ccgaggcgat ccagggcccc tggctgatgg agcagatggc ggcgcaggcc
     9481 accaactgcg gcgccgagct cgtcttcgac atcatcaccg aggtcaatct cgacggcccg
     9541 ccgttccgcg ccaagggcga cagcggcgac gtcatcgtcg ccgacagcct gatcatcgcc
     9601 acgggtgccg tggcgcgctg gctcggcctg ccgtcggagc agcacttcca ggggcacggg
     9661 gtctcggcct gcgccacctg cgacggcttc ttcttccgcg gcaagaaggt ggcggtcgtc
     9721 ggcggcggca acacggccgc cgaggaggcg ctctatctcg cccagatgtg cgagaaagtg
     9781 acgcttgtcc accgtcgcga cgcgctccgg gcggagaaga tcctgcagga tcgcgtcttc
     9841 gcgcacccga agatcgagat cctctgggat cacgtggtcg aggaggtgct cgggtcggcg
     9901 acgccgaagt cggtgaccgg cctgcgcgtg cgccatgtgg cgaccggcgc cactgcggag
     9961 ctcgctgtcg aaggcgtgtt catcgccatc ggccacgatc cggcgaccga gctcttcaag
    10021 ggccggctcg agatggacgg cgagggctat ctcgtcaccg cccccgacag cacgcgaacc
    10081 ggcattccgg gcgtcttcgc cgctggcgac gtgcaggaca agatctatcg acaggcggtg
    10141 accgcggccg gcaccggctg catggcggcc ctcgaggccg agcggtacct cgcatcgcgc
    10201 gagtaggcgt ctgtccaggc aggccggcgg caagaccgcc ctggagatcg gggccaggag
    10261 atcggggaat gatggactgg gaccgggtgc gcatcttcca cgcggtggcg ggggccggca
    10321 gcttcacccg ggcggccgac cggctgggcc tgagccagtc ggcgatcagc cggcagatcg
    10381 gcgcgctcga ggcgaccatc ggcacgccgc tcttccaccg gcatgcgcgc ggcctgatcc
    10441 tgaccgagca gggcgacgtg ctgctcgagg cagcgaacga ggtggccaag cggctggcgg
    10501 ccgtcgagac gacgctcggc gaggtgcgcg acgaggcggc cggccatctg cgcgtcaata
    10561 ccacggtcgg gctcggcacg gtctggctga cctcgcatct cttcgatttc cgcgagctct
    10621 acccggacat cacgctctcg ctcgtggtca ccgacagcga cgtcgacctc gccatgcgcc
    10681 aggcggacgt ggccatccgc ctgcaccgtc cgacccagcc ggatctcgtg cagcggcggc
    10741 tgatgaccgt gcacacccac atctacggcg gccacaccta tctggagcgg cacggcatcc
    10801 ccgagacggt gggcgatctc gatgcccacg cgctcatcgc ctatggcgac cagtggcagc
    10861 cgccggtgcc gagcctgaac tggatcctgg cggcgggacg cgacgaggac gaggtgcacg
    10921 gccgcgcccc ggcgctgacg gtcaacaatg tctacggcat gctgcgggcc tgcgagtgcg
    10981 ggctcggcct cgcctcgctg ccggactatc tgggtggcac caacgcgcgg ctgcggcgca
    11041 ttctgccgac gctcgagggc ccgagcttca ccgcctattt cgtctatccc gaggagctca
    11101 gggcatcgaa gcgggtctcg gtgttccgcg atttcctctt gcggaaggtg gccgagcagc
    11161 cggtctggta ggcgcccaag ggaccctgcc gcctcagcta tgcaaaaaaa gcagggcgat
    11221 cctgcaaatt cgggctggtt tttcgaatcc gaacacccta cttattgcag tgcagcaaga
    11281 cggtgtctct gagcgatctt gctgccgccg cgatgcggcg cggggtgagg cctgcggcct
    11341 tgcctcagag ggctcccgag ggagcactct ctcagacatt tagcctccct gatctacttg
    11401 gccgggtctt cggacccggc ttttttttga tcaactgctg cccggaaaat gcactacgcg
    11461 cactcgagcg gcgcgtcaag ctgtcgcgat cggcattcgc caattcggca gtgtttttaa
    11521 tgtaacataa aagttatcgc gccgatcaca tgtaaagctg ctccccctgc atggccgcat
    11581 aaagctgtgc cacgaactcg ccatagccgt tgaagggcag tgtcggcacc atctcgttgg
    11641 tgccgagcac ccgctcctcg gcctgcgtcc agcgcggatg gggcacctcg gggttcacat
    11701 tggcccagaa gccgtactcc tccggaacga gcgcctgcca gaagctcacg gggcgcgtgt
    11761 cgctgaacgt gaagcgaacg agcgacttca ccgacttgaa gccgtacttc caggggacga
    11821 ccaggcgcaa aggcgcgcca ttctgccgcg gcatgggctc gccatagatg cccgtgccga
    11881 tgagggtaag gtcgttcgtg gcctcttcga tggtcagccc ctcgacatag ggccagggat
    11941 accagaactg cttctggccc ggcgccatgt cgctgtcctc gaaggtcgtc atgaccacgt
    12001 acttcgccgc ggagagcggc cgggcgagct cgaccagcgc cttcatcggg aagccggtcc
    12061 aggggacggc catcgaccag gcctcgacgc agcggtggcg gtagaggcgc tcctcgaccg
    12121 gcatgcggcg aatgagctcc tcggcatcga ggacgatctc gtcctcgacg agcccgtcga
    12181 tggtgacggc ccagggccgg agcctgagcg cctgggcggc cagcgagatc tgcttgtggc
    12241 tgccgaactc gtagaaattg ttgtaggtcg tggcgagctc gggatcggtc agctcgcggt
    12301 cgagcacgta ggtctcgttg cgcggcgcgg ggtagagctc ggcggcgatc ggcgcctcct
    12361 ccgcggcccg caggccccgc ggcagcgccg ccagcatggc gccggcaccg cccgcggcga
    12421 ggagttgccg ccggttgaca tagacggatt tcggggtcgc ggcactctcc ggcagctccc
    12481 aacccttgcg ggtgcgtgtc aacatcgccg actctccatt tcgcctcgat ccgaactagg
    12541 gcgggtgcgg gtccacggca actcaagcct ctgcgatcag ccgtctcgtc gggttggccg
    12601 ggcctctatt cggccgccgt gtccaggagg ccgagcatgc cgcccgactg gcggtgccaa
    12661 aggccggcat agaggccgtt ccggcgcagc agctcggcgt ggctgccact ctcgacgatg
    12721 cgacccttgt ccatcacgac gaggcggtcg agcatggcga tggtcgagag gcgatgggcg
    12781 atggcgatca ccgtcttgcc cgccatcaac gactggagct gctcctggat cgcggcctcc
    12841 acctcggaat cgagggccga ggtggcctcg tcgaggatca ggatcggggc gttcttcaag
    12901 agcacccggg cgatggcgat gcgctgccgc tggccgccgg agagcttgac gccgcgctcg
    12961 ccgaccatgg cgtcgagccc gtgccggccc ttgaggtcgg tgagatcttg gatgaaggtg
    13021 tcggcatggg cccgctcggc ggcgcgctcg atctcctcat cggtcgcatc ggggctgcca
    13081 tagcggatgt tgtcgcggat cgagcggtgc agcagcgagg tgtcctgggt gaccatgccg
    13141 atctgctggc gcagcgattc ctgctgcacg gtggcgatgt cctggccgtc gatcaggatc
    13201 cggccggatt ccggatcgta gaagcggagc agcaggttga cgagggtcga cttgcctgcc
    13261 cccgagcgac cgacgaggcc gaccttctcg cccggccgga tgtggagcgt gagcccctcg
    13321 atgatgccac cgggccggcc gtagttgaaa tgcacattgt cgaagcggat ctcgccctgc
    13381 ggcacccgca gcggcggtgc gcccggccgg tcgacgatgg tgtgcggccg cgccagcgtg
    13441 gtcatcccgt cctgggtcgt gccgatgttc tcgaagagct tgccgacctc ccagaggatc
    13501 cactgcgaca tcgcccggat gcgcatgacc acgctgacgg cgaccgcgat ggcgccgagc
    13561 gagatgagct cctgatgcca ggcgaggacg gcggtgccgc cgatgccggc gagcagcagc
    13621 gagttcaggc agtgcagcga gaaagtgagc atggaggcga gccgcatctg ctggtggacg
    13681 gtcaccatga agccgtccat cgcctcccgg gcgtagacct gctcgcggcg gctgtgggcg
    13741 aagagcttga ccgtctggat attggtgtag ctgtcgacca cccggccggt catctcggcc
    13801 cgcgcatcgg cctgggccat cgagacccgc cggagcctgg gcacgaagta gcgcaggatg
    13861 ccgaggtagc ccgcgagcca gaggacgagc ggcagcatca gccagagatc ggcctgaccc
    13921 aacaggacca tggcaccggt gaagtagacg acgacgtaga ccatgacctc ggtcagcgtg
    13981 atcacggtct cgcggatggc gagggccgtc tgcatcacct tctgcgacac ccggccggcg
    14041 aactcgtcct ggaagaagct caggctctgg ccgagcgcgt agcgatgcgc cagccagcgg
    14101 gcgagcatcg ggtagttgcc gaagatcgtc tggtggagga gcaggttctg cagcagcacg
    14161 aagaccggga agacgacgac tatcagcagc accatgccga tcagggtcgg gccgtggtcg
    14221 gcgaagaacg tggcccggtc cgccccggcc agcgtgtcga caagggagcc gaggaagccg
    14281 aggaacacga tctcgagggt cgagagcgtg gccgtgacca ccgccatgat ggcgatccat
    14341 ggcgccaccg gccgcgtgaa gtgccacagg aacgggatca gccggtccgg cggccgggtc
    14401 ggctcggcct ccggaaaggg atcgatcagg gtctcgaaac gacggaacat gaagctcacc
    14461 aagctgcgca ccggcacctg ccgccaaaat ccgggcggcc ggtcacaagg ccgctctata
    14521 gccccgatcc tgcgccggcg acacgaggag gtgctaaggg cacgagccgc aaggtagagc
    14581 ggcgaattga ttattcagcg gcgggaaccg gcgacaggcc gccgcatttg aacttcggcc
    14641 ggggcccacc aactctggcg tcaggatgcg tcgacgattc cgaccggcga atgaccggct
    14701 atagtgggaa ccggcggcga ttcggcaggt ttcttgcttc gggacggtcg ggtcgggcgg
    14761 tgggcccaaa cggatcctgg agatccgggc gacgcgggat ggaggaaatg tatggatcgg
    14821 gccttgctcg atgccgtgaa tcggcgctcg atgttgcgga tcgccgccgg ttgcggcgtg
    14881 ctcgcggcga gcgccggctt ggcccgaacc gcggctgtcg gcaccgccga cgccgcagcg
    14941 gccgagcccg acggcccttt tacattcgag acggtgatcg cacaggcgag gcggctcgcc
    15001 ggcgaggagc acgaggcggc gaaaatcgag ctgaagggtc ccttcgccga tctcgactac
    15061 gaccactacc gggcgatccg ctttcgcccg gagcggcggt tctggcagga cgagaagcgg
    15121 ggcttccagc tcgatctcct gccgccgggc ttctactatc aggaccggat cgacctggcg
    15181 ctcgtggtgg acggggtcgt ccacccgctc gagttcgatc cgggcctctt ccactaccac
    15241 cccgactatt tccccttccc ggacggcgct gcccccgggg atgcgccgcg cgacctcgcc
    15301 tattccgggt ttcgggtgcg cttccccatc aaccgccccg acgtcatgga cgagttcctg
    15361 gtcttccagg gtgccagcta ttttcgtgcc gtggcgcgtg accagctcta tggcctctcc
    15421 gcgcgcgggc tggcgctcgg cacgggctcc cccgagggcg aggagttccc gctctttcgc
    15481 aagttctgga tccacgagcc acaggtcgca gcgaccagcc tgacgatcca ggcgctgctc
    15541 gacagcccgt cggtcacggg cgcctacgag ttcatcgtcc gcccgggtgc ggagacggtc
    15601 atggacgtgc gctcgatcct gttcccgcgc gccgatctca cggctgccgg catagcaccc
    15661 ctgacctcga tgttcttctt cggcccgaaa tcgcgggccc gcatcgacga ctaccgcgat
    15721 gccgtgcacg acagctcggg cctgcagatg gtgaccggtg cgggcgaacg catctggcgg
    15781 ccactcgaca acccgctgac cctgcagttc tcggcgttca gcgaccacga cccgcaaggg
    15841 ttcgggctga cccagcgcga gcgggcgttc acccattacg aggattccga ggcccgctac
    15901 gagcggcggc cgagcgcctg ggtggcgcca tcgaatgcct gggggcgcgg ctcggtggtg
    15961 ctcgtcgaga tccccaccga caccgaattc aacgacaatg tcgtggcctt ctggcggccg
    16021 gccgagccgc tcacccgggg cagcgagcat cccttcgact atcgcctgct gtggagcggc
    16081 gagccaccgg acgagacgcc gctcgcccgg gtcgtcgcga cgcgcggcgg ggtcaaggtc
    16141 gcagtgcccg aggaccgcag cttcatcgtc gacttcgatc tcggcgagct gccattcgag
    16201 gggctcgagc cgcgcgtgac ggcgagcgcc ggcgacatca cgcatgtggc gctcgtgcgc
    16261 ctgccggccg agtccagggc cagggccatc atcaccttcc atcccgggga tgcggggctg
    16321 gccgagctgc gtctctcgct ggtcgacgct gaagggcgga ccgcttccga aacgtggctc
    16381 gcacgatgga ccccgcggtg acccggatcg accaggcacc ggtcccgccg ccggcgccga
    16441 tggcgatgcc ggcacaggac ctgcggcgcg tgccggagcg gcgctttcgg tcgcagggct
    16501 ggcgcgtcca tgtggcccgg ctcgtcgcct tcggcggggc gctggcgctg acggtgctgg
    16561 gtgcccagca aatgggccgg gtcttcgatc cggcgcatgt cagtgccctg cagacgaccc
    16621 tcagcgtgct cttcaccctg accttcggct ggatcgcctt ctcggcggcg gcggcggtgg
    16681 ccggcctgct cgccgtgcga ccgcagcgac cggcggcggc gaaggcgggc gaagggccgc
    16741 tcgggcggac ggcgatcgtc atgccggtct acaacgagga cccggtggcg accgccgggg
    16801 cgctcgccgc catgggccag ggcctcgccg agctcggcca tggcgaggac ttcgagatct
    16861 tcatcctctc ggacacgcgt gacgcgaccc actgggcccg cgagacagcg gtcttcgcgg
    16921 cgctgcgcca gcggctcgag ggccgcatgg cggtctggta ccgccgtcgg gcgcggaaca
    16981 cggcgcgcaa ggccggcaac ctgcaggact tcatcgagcg ctggggcggc cgctacgact
    17041 acctcctgtc gctcgacgcc gacagcctga tggcgcccga gacgatcgtc gagatggccc
    17101 ggcgcatggc ggccgagccg cggctcgggc tcctgcagac ggtgccgatg ctggccggcg
    17161 gcgagacgct ctacgccagg ctgcagcagt tcgccggccg gctccacggc ccggcaatcg
    17221 cgcgcggcac cgccgcctgg cagggcgacg acggcaatta ctggggccac aacgcgatgc
    17281 tgcgcacggc ggccttcgcc cagagcgccg gtctgcccga gctgcggggg cgagcaccct
    17341 tcggcggtca tgtgctcagc cacgatttcg tcgaggcggc gctgctgcgc cgggccggct
    17401 ggatcgtccg catggacccc gacctcgatg gcagcttcga gggcgcgccg ccgtcgctcg
    17461 cggacgccgc cgcgcgcgac cgccgctggg cgcagggcaa cctccagcac ctcaaggtga
    17521 tcggtgcgcg cggcctgcgc tggccgagcc gggtgcattt cctcatcggt gtcggctcct
    17581 acctcgcctc gcccctctgg ctcgccatga tcctcgtcgg cctcgtcctg accgcgcagg
    17641 cgatcttcat ccagcccgag tatttcccga acacctacca gctcttcccc gaatggccgc
    17701 gtttcgacgc gctccgcatg cgctggctgt tcatcctctc gatcggcctg ctgctcctgc
    17761 cgaagctttt tggcctggcc cgggcactcg cccaccggcc gacccgtgaa cgcttcggcg
    17821 gagcccggcg gctgctcccc ggggcactcg ccgagatcgt gctctccgcc ctctacgcac
    17881 cggtcatgat gctgatgcag acacgccagc tcttcgagat cctggccggc cgcgacagcg
    17941 gctggtcgac ccaggcgcgg aacggcagcc ggatcacctg gcgcgatgcg ctgggccgcc
    18001 actggcgaca cgcgctggcc ggcctcgtgg tcagcgccgg gctgctctac gcggcaccga
    18061 ccctcctgcc ctggctcgcc ccggtcctgg tggggctggt gctcgcccca tggctctgcc
    18121 gggtcagcgg cgatccggcc gtcggtgccc gcttcgcacg gctcgggctc ctggtgaccc
    18181 ccgaggagac ccggccgcat cggatcttcg cggcggctcg cacggaggcg gcgcacctcg
    18241 ctccggccgg cgtcgtcggg ctcggcgatc tcgcccggct ggaaggtgcc cgtgccgctc
    18301 atgtggccag tctgaccgaa gcggaccggg ccgagctcga ccccctcgcg gtcatcaccg
    18361 cccgggccaa gatccaggcg gcagcgacca ccgagcaggc gctggactgg ctcaccgagg
    18421 ccgagcgacg ggcactcctg gccgttccgg acctgctcga cgcgctggca cggcaaccgg
    18481 tgctgctggc ggcctgagcg ccagctcggc gggcgcgatc agcgcgaaga cgtggatggg
//
LOCUS       DAOTFI010000083        18527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3605_length_18527_cov_7.299047, whole genome shotgun sequence.
ACCESSION   DAOTFI010000083 DAOTFI010000000
VERSION     DAOTFI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18527
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_18527_cov_7.299047"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..900)
                     /locus_tag="P5558_10245"
     CDS             complement(94..900)
                     /locus_tag="P5558_10245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24732.1"
                     /translation="MMGEPGWVRRLVAASGAAVLVFLCLPILIVVPMSFSSASSLEFP
                     PPGFSLRWYEAFFGDPRWLEAAGTSLALAALASSFALVLGTIAAYGLVRGRFKGRPLV
                     ELNFMAPLILPQIITGVALYIFFARIGLLGSFTGLVIGHTVLAVPYTVLVMTVAIRAF
                     DRDIERAALSLGAAWPVMAWRVLVPNLMPSAAAAWIFAFIISFDEVIVTIFLAGAHET
                     IPKRMFNELVLQVNPTITAIATLLIGISVVTVLAVTTLARRAGLLAKIAD"
     gene            complement(897..1730)
                     /locus_tag="P5558_10250"
     CDS             complement(897..1730)
                     /locus_tag="P5558_10250"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24733.1"
                     /translation="MATIQLRPGPSGVGFVLPLGLVMLLAFNVPVVLMLAWGFTSPKG
                     VLVPWIDMVERTVYLRVLVNTFRIALIATATCAILGYPLAYWLCCLRGRARMIGLALV
                     VLPFWVSILVRTYAWIVVLGNNGVVNRSLLGLGFVDEPLAILYNELGVIIGTTNVLLP
                     FLVLPLFAAMVRIDRRLLMAAAGLGAGPWTVFRRVFLPLTLPALAAGCILVFILTLGF
                     FITPAILGGGRVPMIANMIDMLINRLPRWELAAALSTVLLVATFVCYALYLRIGRETV
                     R"
     gene            complement(1744..2781)
                     /locus_tag="P5558_10255"
     CDS             complement(1744..2781)
                     /locus_tag="P5558_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY24734.1"
                     /translation="MILPRRHVIGGAAALAGTATLGRLARPARAAVSEFRMTEAGGAS
                     GDSIQAGYIEPFTGKTGIKVVRESPSGLGKLRAMVEARSITSALLELSSPELEQAKAL
                     DLVEALDWAAIDPLPIFPDAKDEYGFGYQYYSTIMAWHPDAKAPSTWAEFFDVESFPG
                     KRALPDYAAYILAMAAMADGVPPEELYPIDLDRAFGKLESIKDDVAVWWQAGAQPPQL
                     IKDNEVQYAVAWSGRVAGQDGVRFSFAQGQLDLAFFVVPKGADPEVTAAAMGLLHEMT
                     VPENQAVAAAVIPYTGNSPDLDPLLPQDSLNQFPTTAENRSIQVLSDARWWFENADEV
                     ELRWQEFKLSL"
     gene            2914..4263
                     /locus_tag="P5558_10260"
     CDS             2914..4263
                     /locus_tag="P5558_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY24735.1"
                     /translation="MNETIGYGSNSALQAQLAEVEAAYVAANPESRAAYAAAAGPMPG
                     GNTRTVLHFSPFPLTFAKGEGCRLWDVDGHGYRDFLGEYSAGLYGHSEPVVQAAIREA
                     LEQGIALGGPNRYEARLAAEITARFPSIDQVRFTNSGTEANILAFAAARLATGRDAVM
                     VMAGGYHGGVLYFGQHPSPINLPGPWVQGIYNDVEATGAIIEEEAERLAAIVIEPMMG
                     SGGGICAEPGFLAMLRAKADRHGIVLIFDEVMTSRMSMGGMQERLGVTPDMTTLGKYL
                     GGGLSFGAFGGREELMRRFDPRRPDAVSHAGTFNNNVCSMAGGLAGLEQVLTRPVLDR
                     LWADGEALRARLNEVAQARDVPVLVLGTGSIMGFHFKRGTIRRPQDVWPTDALAARTQ
                     NDLYALLHLSLIQAGCYIARRGYVTLSLPMEEADRAALVAAFETFLAEHGELIRELV"
     gene            4430..5374
                     /locus_tag="P5558_10265"
     CDS             4430..5374
                     /locus_tag="P5558_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24736.1"
                     /translation="MRARPRTIAVLVLAGVLVLASGTLQPGQAGPSDEKLPPPKAGAK
                     QPATVPYEQLPPNVQYGQLPSTNPQPGNAPMLGTFENRDEVSQRNRLDKAARGQREKP
                     LEMGTASKQVQKLPPPPRRIEMGKASKHVQKLPPTRIDPKPIDNGPERMAPRRPRKGQ
                     PGATAGASPEPTTSNPARQRTSSTGPVAAQQSSDSANASTSRRAAPAQAGATGQRTLQ
                     YVKLPQEEAANSNASTSRRTAPAGAGAAATGAGGAARPAQVTKGPSVAERRAKFETPS
                     KPAATMQARGQRMSSTTPPANPTEPAALPKARKRKGGP"
     gene            5432..9115
                     /locus_tag="P5558_10270"
     CDS             5432..9115
                     /locus_tag="P5558_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAC/perforin domain-containing protein"
                     /protein_id="HRY24737.1"
                     /translation="MFSRLAPLACALLAALLAGPAGAQAADPAAAPVAVTLVNGGAAP
                     VDVLWVDDDGEPAPLDMTIEPGWQQVLNGWPGTELLFRESGTGDEMAPFFSVTVEEQA
                     PAEPIVIPDPRLLRTAALTIANPLGVEVVLYWLPDEAPGETPEQTGAAWTEFDRVAAG
                     GSVAYELDPLTPLRLRAEDGSLEVDFEVGLDSEQLLDLGTLTDPDMPTAEVTLTNESA
                     RPVVLQELRPGVTQRLGDVPAASAATVDAVVGADLRVVAGDGDLEEMGLYHVTDAADQ
                     VVLIEDAAEAGVVIPPATGIDLSLLPEDQRASAAGFLEKAGAIFAGRDDVALYVAYNG
                     SDQPIWLAGLDGETIKGAKQMKPRSIGSVIGKVGDYSIAWPADVTPDKVTRGDVFASI
                     LVATDMPKVVDVSPPTPVRLGVGNEVCKPVDLLRVDPFGEEHLVTRLEPMGGTGEPLE
                     LPANSFLVARAGSASVLAGQALWSGRLDDSATQRIAIEPGVASGYDPMRVSVAGGAER
                     TVNLHNSTSRGIDLFAVDENGRELVLTDAPIGAGKTQVFYIAEGTVIVARRPNVPPSI
                     SEYVRLRVGDAPTQTIDIGALRQSRGVVGSWIDLPRPALAENNEVGTTFVSFVGRGGM
                     AIAGSTNEGDAELGGAARFEGCADDREAFGYWYPRRFEILCGETTHRDADGRETTSFG
                     RFAAQRAASGELVLRRGYCESEPTRSSTWYRLPQHYDDMDPSLEAFQTGFYQLTWAPR
                     SFDWMGRGFDLLRTDPLDYGSTERGVTSESPLFDFVFEDRAGSAGQTASKAIFGVRNL
                     NEQGRSDRCDRDEKLVRTRADLEDFAAKSYGGSVGVPGIVSFSLSKSHQEINTSATGS
                     ERAFILERCDVRLHALKMDLRWPDRRGEDSLRQPLETGFRQAVGQLPLEAGDGKAYYA
                     FLDKYGTHFAERIVYGGTFFAETEVTKSTYQIAEKEGDKLSLAAEGTIKKVSLGGTYE
                     NETSKGRANDNGHSSSTYRKWSVGGTGSDVYPVWAPTVPDNPAPIDVSFRPVYDILTP
                     VFFPDDPNIGLKNALLRQATASYFRQHGKGDLTSDPKGLDIRDPRSICVRMDVVEYTS
                     TSGRSGDRFVSDLSAGFVDGARNVVTDAEPFQLVHAPGNLNDARAVDPNLDPERCTPP
                     VTASYVRSGSIYLHGVIGQYPDGWGRPSTTAELSRHFPKADQTYPLRRLEPGTPRVFE
                     GMIQLPEGQARYKLRVRAVDQAT"
     gene            complement(9200..9700)
                     /locus_tag="P5558_10275"
     CDS             complement(9200..9700)
                     /locus_tag="P5558_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRY24738.1"
                     /translation="MGKSGLDVVKVGDRYTFARTVGESDVMLFAGLTGDFSDTHVNEQ
                     YMRQRSSVGGRIAHGALLIGYMSTASTMSIAHVIHDDDLTDFPVSAGYDRIRFLRPVL
                     LGDTVTVHYVVETVDKAGGRSIAGIEVSNQRGELVAVGRHVMRWTRKLKAPVSPPSAR
                     VGARRS"
     gene            9817..10413
                     /locus_tag="P5558_10280"
     CDS             9817..10413
                     /locus_tag="P5558_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRY24739.1"
                     /translation="MTGNKVLIIVGDASETVDTLYPYYRLQEAGMTPVVAGPEKRRFQ
                     MVLHEIRPGWTITREWEGYQIEAEVAFAEIDPADYVGIFFSGGRAPEYIRDDPDLIRI
                     TRHFFATGKPIASVCHGVEIPARADCVRGRRMATVAKCRFDLEVCGGIYVDQPCVVDG
                     NLVSGRTFHDHGHYMGAWIELLRQHAVGQRPKAAAGAA"
     gene            10410..11180
                     /locus_tag="P5558_10285"
     CDS             10410..11180
                     /locus_tag="P5558_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HRY24740.1"
                     /translation="MTARTAIVTGGRRGLGRAIAMELAQSGFDVAIADLDRDDDATET
                     IAAVEAAGRQARFIAVDIADLDSHEAVLAAAASLPGPLCTLVNNAGVSSMVRGDMLEL
                     SVESFDRSIAVNLRAAFFLTQLFARRLGPADPAGPFRSIVNITSVNAADVLAPTRADY
                     GMSKAAMSMMSRLFALRLAELGIHVYEVRPGIMRTGMTAPSAARYDAFVASGGVPIAR
                     WGTPDDVGRAVAMLANGGLGFTTGSAIEVDGGMHLHAV"
     gene            complement(11686..11919)
                     /locus_tag="P5558_10290"
     CDS             complement(11686..11919)
                     /locus_tag="P5558_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24741.1"
                     /translation="MADSISRTISAGPAQAARRPEARSAVAVMSGARAATLRAVKTGA
                     MARRCQRHWAPSEVKRLAPTAERSMRIMISDFG"
     gene            complement(12864..12939)
                     /locus_tag="P5558_10295"
     tRNA            complement(12864..12939)
                     /locus_tag="P5558_10295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12904..12906),aa:Ala,seq:ggc)
     gene            complement(12986..14386)
                     /locus_tag="P5558_10300"
     CDS             complement(12986..14386)
                     /locus_tag="P5558_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY24742.1"
                     /translation="MSRLWPRSMTGQLVAILLLALVLAQLATFAILQGERQSTVEAIG
                     REQVLERAAALARLLDETTEPVERRRALRAFSTRRLRFWLADEAVVEEQGEGPAVRLA
                     RKLADLLEGEGEHEVLTAMGEIGRQPHGMPRWHRQLHDAGVEPRQPTDPGLLVAIRVG
                     PESWLNAAMLLPPERPAFGLAPLVALVAAALAVSLAAVLGLRRLTRPLAALAAAADAV
                     GRGEPVELPADRGPVEIRRTATAFNRMQARIGRAMAERTRLLAAVSHDLRTPITTLRL
                     RAEMVDDPELQERMLATLDEMQALTEAGLLLARGTSAEEPTVAVDLGALVESLAADQA
                     DLGAAVTVEPQESGRIVLRCRPLALKRALRNLVENAVRYGGSARLALAGDGQRVTLTI
                     DDDGPGLPEDLLERVFDPFVRGEASRSPETGGAGLGLAIARTILRAHGGDVALANRKG
                     GGLRATVTLPTGGDDI"
     gene            complement(14383..15105)
                     /locus_tag="P5558_10305"
     CDS             complement(14383..15105)
                     /locus_tag="P5558_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY24743.1"
                     /translation="MSDGPHLLVVDDTRDVRDALRQYLQKQGHRVTTADSAQAARREL
                     ARAAIDLVILDVMMPGEDGLVLCRELRARGNLPIILLTAMAEETDRIVGLEMGADDYV
                     TKPFAPRELLARIKAVLRRAQSLPHRPGAPATAGRVRFDRWLFDPGTRDLVDEEGVTV
                     PLSTGEYLLLTALLRHPGLVLSRDQLLDLTRGREAQPFDRSVDNQVSRLRKKLERDPR
                     NPLLIKTHWGGGYSFTGRVEPA"
     gene            15282..15785
                     /locus_tag="P5558_10310"
     CDS             15282..15785
                     /locus_tag="P5558_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24744.1"
                     /translation="MSRTSILATAALGLVALAGGQALADGGQGGRHMGGSGLMGGGQM
                     MQGFDRDGDGSFTQEDVDAVRGERFAEFDADGDGALSLAEYEALWLDAMRERMVRGFQ
                     HHDRDGDALVTVEEFTLTTGNLVERHDRNGDGVVTRDELQRRPHGEGRRGGQRPRQAP
                     PAGDSPN"
     gene            complement(15817..16230)
                     /locus_tag="P5558_10315"
     CDS             complement(15817..16230)
                     /locus_tag="P5558_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HRY24745.1"
                     /translation="MPKPELGTKRTCLACGARFYDFGKTADLACPACGAVFDLELLAR
                     ARRPRTVTRAAPAAAAVEEEVDEAETDDTEDDVEEVEDDVDDTDEEEADEPEVVETED
                     DEEEDALIEDASELGDDDALGDVIDKDADGEEERR"
     gene            16458..17783
                     /gene="aroA"
                     /locus_tag="P5558_10320"
     CDS             16458..17783
                     /gene="aroA"
                     /locus_tag="P5558_10320"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRY24746.1"
                     /translation="MLLQAAPHGALQGDAVVPGDKSMSHRSLLLAALAVGESRITGLL
                     EGADVMATAGALRALGATVERQPDGTWTVRGCGVAGLAEPADVLDLGNAGTGARLLMG
                     LLAGHAFTTFLSGDASLRSRPMRRVTEPLVAMGASVLGRSGGRLPLAITGRNDLVPIT
                     WRSPVASAQIKSAVLLAGLHAPGRTTVVEPLPSRDHTERMLAAMGAKITGEAGADGGW
                     TVAIEGEAELAPQAFLVPADPSSAAFPLVAALTAPGSALTLPGISVNPLRTGLLATLR
                     EMGAKLELANERSVAGEPVADLEVRASRLVGVDVPAARAPSMIDEYPILAVAAAFAQG
                     TTRMRGLAELRVKESDRLLLVAEGLAACGVEVAVEGDDLLVTGPVRATRAARIDAHHD
                     HRIAMSFLVMGGLGSAPVTVAGAETIETSFPGFADLMNRLGAGLEAIEP"
     gene            17780..18436
                     /gene="cmk"
                     /locus_tag="P5558_10325"
     CDS             17780..18436
                     /gene="cmk"
                     /locus_tag="P5558_10325"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRY24747.1"
                     /translation="MSRAVIVAIDGPAGSGKSTLAKRLAATTGLRFLDTGLLYRAVAA
                     RLRATGGSPLDAAAAAAAARTIEPEGLDPDALHGEGIGGDASRVAALPEVRAALLELQ
                     RRFARTPPGAVLAGRDIGTVVCPDATLKIFVTASIEERARRRFEELRARGEAPIHERV
                     LLEVAERDRRDRERAVAPLSVAEDAVVIDTSTLDVEQSHAALEAAYAARIGSSANRTS
                     "
BASE COUNT         2808 a         6722 c         6495 g         2502 t
ORIGIN      
        1 cccggcgccg acctcccagc cggaggtctg ctcgaaggtc gcgagcgcct cgggccccct
       61 tccggcaagg gttccccgtc gccggcggca gcctcagtcg gcgatcttcg ccaggaggcc
      121 ggcgcggcgg gcgagcgtcg tgacggcgag gacggtgacg acgctgatcc cgatcaagag
      181 cgtcgcgatg gcggtgatgg tcgggttcac ctgcagcacc agctcgttga acatgcgctt
      241 gggaatggtc tcgtgggcac cggcgaggaa gatcgtcacg atcacctcgt cgaaggagat
      301 gatgaaggcg aagatccagg ccgccgccgc cgatggcatc aggttgggca ccagcacgcg
      361 ccatgccatg accggccagg cggcgccgag cgagagtgcc gcgcgctcga tgtcgcggtc
      421 gaaggcgcgg atcgccacgg tcatgaccag cacggtgtag ggcacggcga gcacggtgtg
      481 gccgatcacc aggccggtga agctgccgag caggccgatc cgggcgaaga agatgtagag
      541 cgccacgccg gtgatgatct gcggcaggat caaaggcgcc atgaagttca gctcgaccag
      601 cggccggcct ttgaaccggc cgcgcacgag gccgtaggcg gcgatcgtgc cgaggaccag
      661 ggcgaagctc gaggcgaggg ccgccagggc cagcgacgtg ccggccgcct cgagccagcg
      721 gggatcgccg aagaaggcct cgtaccagcg cagcgagaat ccgggcggcg ggaactcgag
      781 cgagctcgcc gaggagaacg acatcggcac gacgatgagg atcggcaggc agaggaagac
      841 cagcacggcg gcgccgctcg ccgccaccag ccggcgaacc cagcccggct cgcccatcat
      901 cgtaccgtct cgcggccgat gcgcagatag agcgcgtagc agacgaaggt ggccaccagc
      961 agcacggtcg agagggcggc cgccagctcc cagcgcggca ggcggttgat cagcatgtcg
     1021 atcatgttgg cgatcatcgg cacccggccg ccgccgagga tggccggcgt gatgaagaag
     1081 ccgagcgtca ggatgaagac gaggatgcag ccggcggcca gcgccggcag cgtgagcggc
     1141 aggaagacgc ggcgaaagac ggtccagggc ccggccccca gccccgccgc cgccatgagc
     1201 agccgccggt cgatcctgac catggcggcg aagagcggca ggacgaggaa gggcagcagc
     1261 acgttggtcg tgccgatgat gacgccgagc tcgttgtaga ggatggccag cggctcatcg
     1321 acgaaaccga ggccgagcag cgagcggttg accacgccgt tattgccgag gacgacgatc
     1381 caggcatagg tgcggaccag gatcgagacc cagaagggca gcacgacgag ggcgaggccg
     1441 atcatccgcg cccggccccg gaggcagcag agccagtagg cgagcggata gccaagaatg
     1501 gcgcaggtgg ccgtggcgat cagtgcgatg cggaaggtgt tgacgagcac gcggagatag
     1561 accgtgcgct cgaccatgtc gatccagggg accagcaccc ccttggggct cgtgaacccc
     1621 caggccagca tcagcacgac cgggacgttg aaggcgagca gcatgaccag gccgagcggc
     1681 agcacgaagc cgacgccgct cggccctggt cgaagctgga tggtcgccac gcctcagagc
     1741 gctctacagg gagagcttga actcctgcca gcgcagctcc acctcgtcgg cgttctcgaa
     1801 ccaccagcgg gcgtcggaga ggacctggat ggagcggttc tcggccgtgg tcgggaactg
     1861 gttcaggctg tcctgcggca gcagcgggtc gaggtcgggg ctgttgccgg tataggggat
     1921 gaccgcggcg gccacggcct ggttctccgg cacggtcatc tcgtgcagga gacccatggc
     1981 ggccgccgtc acctcgggat cggcgccctt gggcacgacg aagaaggcga ggtcgagctg
     2041 gccctgggcg aagctgaagc gcacgccgtc ctggccggcg acgcggccgg accaggccac
     2101 ggcatactgc acctcgttgt ccttgatgag ctgcggcggc tgggccccgg cctgccacca
     2161 gacggcgaca tcgtccttga tgctctcgag cttgccgaag gcgcggtcga ggtcgatcgg
     2221 gtacagctcc tccggtggca ccccgtcggc catggcggcc atggcgagga tgtaggcggc
     2281 atagtcgggc agcgcccgct tgcccgggaa gctctcgacg tcgaagaact cggcccaggt
     2341 cgacggcgcc ttggcgtcgg ggtgccaggc catgatggtc gagtaatact ggtagccgaa
     2401 gccgtactcg tccttcgcat ccgggaagat cggcaggggg tcgatcgccg cccagtccag
     2461 cgcctccacc agatccagcg ccttggcctg ctcgagctcc gggctcgaga gctcgagcag
     2521 ggccgaggtg atgctcctcg cctcgaccat ggcgcgcagc ttgccgagcc cggacgggct
     2581 ctcgcggacc accttgatgc cggtcttccc ggtgaagggc tcgatgtagc ccgcctggat
     2641 cgaatcgcct gaggctccgc cggcctcggt catccggaac tcgctgaccg cggcccgggc
     2701 cggacgggcc agccgcccga gcgtggccgt tccggccagg gcggcggcgc cgccgatcac
     2761 atgtcgccgt ggcaagatca tcaggggtcc tcccgtcgtt ggggcgctgc ccaccagaat
     2821 gttcgtgcgc ccgatggaaa attggtagaa gcaaagaggt cgggccgcaa ggtcgcccac
     2881 ggtcgggccg tcatggggat ggtggaggaa agaatgaacg agacgatcgg ctacggctcc
     2941 aattcggcgc tccaggcgca gctcgccgag gtcgaggccg cctatgtcgc ggccaacccc
     3001 gagagccggg cggcctatgc cgcggccgcc ggcccgatgc ccgggggcaa cacgcgcacc
     3061 gtgctgcatt tctcgccctt ccccctgacc ttcgccaagg gcgagggctg ccggctttgg
     3121 gacgtcgacg gccacggcta ccgcgatttc ctcggcgagt actcggccgg cctctatggc
     3181 cactcggagc cggtggtgca ggcggcgatc agggaggcgc tggagcaggg catcgcgctc
     3241 ggcggcccca accgctacga ggcccgcctc gccgccgaga tcaccgcgcg ctttccctcg
     3301 atcgaccagg tccgcttcac caattcgggc accgaggcga acatcctggc cttcgccgcg
     3361 gcccggctcg ccaccggccg cgacgcggtc atggtgatgg cgggcggcta tcacggcggc
     3421 gtgctctatt tcggccagca cccgagcccc atcaacctcc ccggcccctg ggtgcagggc
     3481 atctacaacg acgtcgaggc gaccggcgcc atcatcgaag aggaggccga gaggctcgcc
     3541 gccatcgtca tcgagccgat gatgggctcg ggcggcggca tctgcgccga gcccggcttc
     3601 ctcgccatgc tgcgcgccaa ggccgaccgc cacggcatcg tgctgatctt cgacgaggtg
     3661 atgacgtcgc gcatgagcat gggcggcatg caggagcggc tcggcgtcac cccggacatg
     3721 accacgctcg gcaagtatct ggggggcggg ctcagcttcg gcgccttcgg cggcagggag
     3781 gagctgatgc gccgcttcga tccgagacgc cccgacgccg tctcccacgc cggcaccttc
     3841 aacaacaatg tctgctccat ggccggcggc ctcgccgggc tcgagcaggt cctcacccgc
     3901 cccgtgctcg accgcctctg ggcggacggc gaggcgctcc gcgcccggct gaacgaggtg
     3961 gcccaagcgc gcgacgtgcc ggtcctggtg ctgggcaccg gctcgatcat gggcttccac
     4021 ttcaagcgcg gcaccatccg ccggccgcag gacgtctggc ccaccgacgc gctcgcggcc
     4081 aggacccaga acgacctcta cgcgctcctc cacctctcgc tgatccaggc cggctgctac
     4141 atcgcccgcc gcggctacgt cacgctctcg ctgcccatgg aagaagccga ccgcgccgcc
     4201 ctggtcgcgg ccttcgagac cttcctcgca gaacacggag agttgatccg cgagctggtc
     4261 tagcgatccg gcgaatgacg atggaagctg aaccattcga ggttgcgcga gactggctca
     4321 ccgagtatag tcgattgcaa gtggtcgaaa gaatcgtgcg aataacgccc tccgagtagt
     4381 agatttcgag cgctcgaacg ggcgcgcgac agggaaagga aaccccagaa tgcgggcaag
     4441 accacgcacg attgccgttc tcgttctggc gggcgtcctg gtcctggcga gcggaacctt
     4501 gcaacccggc caggcaggtc ccagcgacga gaagcttccg ccgccaaagg cgggtgccaa
     4561 gcagcctgcc acggtgccgt acgagcagct tccgccaaac gtccaatatg ggcagcttcc
     4621 ttcaacgaac ccgcaacctg gcaatgcccc catgctcggg accttcgaga acagggacga
     4681 ggtcagccaa aggaaccgtc tggacaaggc tgcccgagga cagcgggaga agccgctcga
     4741 gatgggcacg gcctcgaagc aggtgcaaaa gctgccgccg ccgccgaggc ggatcgagat
     4801 gggcaaggcc tcgaagcacg tgcaaaagct gccgccgacg cggatcgacc caaagccgat
     4861 cgacaacgga cccgagcgga tggcgcccag gaggccgcgc aagggccagc cgggcgcgac
     4921 ggccggcgcc tcgcccgagc caacgacatc gaacccggct cgccagcgga cgtccagcac
     4981 cgggccggtc gcggcacagc agtcttcgga cagtgccaac gcatcgacct cgcgccgcgc
     5041 agcaccggct caggcgggtg ccacggggca gcgcacgctc cagtatgtaa agctaccgca
     5101 agaagaggcc gccaactcca acgcatcgac ctcgcgccgc acggcaccgg ccggtgcggg
     5161 tgccgctgcc accggtgcgg gtggcgcggc gcgtccggcc caggttacca aaggcccgag
     5221 tgtcgcggag cgccgggcca agttcgagac cccgtcgaaa ccagccgcga ccatgcaggc
     5281 cagagggcag cggatgagca gcacgacgcc cccggcgaac cccaccgaac ccgcggcatt
     5341 acccaaagcg cgcaagcgga agggaggacc gtagacctcg accggcgtcg tccctggcgc
     5401 catccggctc gccatgcaaa ggaagcaccc catgttctcg cggctggctc ctctcgcttg
     5461 cgcactccta gcggcgctgc tcgccggacc ggcaggcgcg caggccgctg atccggcggc
     5521 ggctccggtt gccgtcacgc tcgtgaacgg cggcgcggca ccggtcgacg tgctctgggt
     5581 ggacgatgac ggcgagccgg cgccgctgga catgacgatc gagcccggct ggcagcaggt
     5641 gctgaatggc tggccgggga cggagcttct gttccgcgaa agcggcacgg gcgacgagat
     5701 ggcacccttc ttcagcgtga cggtcgagga gcaggcgccg gccgagccca tcgtcatccc
     5761 cgatccgcgg ctgctgcgca cggccgcatt gaccatcgcc aacccgctcg gggtcgaggt
     5821 cgtgctctac tggctgccag acgaggcccc cggggagacc cccgagcaga ccggcgccgc
     5881 atggaccgag ttcgatcgcg tggccgcggg cggatcggtc gcctacgagc tcgaccccct
     5941 cacgcccctg cggctgcggg ccgaggacgg cagtctcgag gtggatttcg aggtcggcct
     6001 cgacagcgag cagcttctcg atctgggcac cctcaccgat cccgacatgc cgaccgccga
     6061 ggtcaccctg accaacgagt cggcgcgtcc cgtggttctc caggagctgc ggccgggcgt
     6121 gacgcagcgg ctcggcgatg tgccggccgc gagcgccgcc accgtcgacg cggtggtcgg
     6181 cgccgatctc agggtcgtcg ccggcgatgg cgatctggag gagatgggcc tctatcacgt
     6241 caccgacgcg gccgaccagg tggtgctgat cgaggacgcc gccgaggcgg gtgtggtcat
     6301 cccgccagcg accggaatcg atctctcgct gctgcccgag gaccagcggg cgagcgccgc
     6361 cggctttctc gagaaggcgg gcgccatctt cgccggccgc gacgatgtcg ccctttacgt
     6421 cgcctacaac ggcagcgacc agccgatctg gctcgcgggg ctcgacggcg agaccatcaa
     6481 gggggcgaag cagatgaagc cgcgctccat cggcagcgtc atcggcaagg tgggcgacta
     6541 ctcgatcgcc tggccggccg acgtgacgcc cgacaaggtc acccgcggcg acgtcttcgc
     6601 ctcgatcctg gtggcgacgg acatgcccaa ggtggtcgac gtctcgccgc cgacccccgt
     6661 gcggctcggc gtcggcaacg aggtgtgcaa gccggtcgat ctcctgcgcg tcgacccgtt
     6721 cggcgaggag cacctggtga cccggctcga gccgatgggc ggcacgggcg agccgctgga
     6781 actgccggcc aactctttcc tggtggcgcg cgccggatcg gccagcgtgc tcgccggcca
     6841 ggcgctgtgg tcgggtcggc tcgacgacag cgccacgcag cgaatcgcca tcgagcccgg
     6901 cgtcgccagc ggctacgacc cgatgcgggt ctcggtggcg ggcggcgccg agcgcacggt
     6961 caacctgcac aactcgacca gccgcgggat cgacctcttc gcggtggacg agaacgggcg
     7021 cgagctggtg ctgaccgacg cgccgatcgg ggccggcaag acccaggtct tctacatcgc
     7081 cgaaggcacg gtgatcgtgg cccgccggcc caacgtgccg ccgtcgatca gcgagtacgt
     7141 ccggctgcgg gtcggcgacg cgccgacgca gaccatcgat atcggcgccc tgcggcagtc
     7201 gcgcggcgtg gtcggctcct ggatcgacct gccgcgcccg gctctggccg agaacaacga
     7261 ggtcggcacg accttcgtct cgttcgtggg ccggggcggc atggcgatcg ccggctcgac
     7321 caacgagggc gacgccgagc tcggcggggc cgcccgcttc gagggctgcg ccgacgaccg
     7381 cgaggcgttc ggctactggt atccgcggcg cttcgagatc ctgtgcggcg agaccaccca
     7441 tcgcgacgcc gacgggcgcg agacgacgag cttcgggcgg ttcgcggccc agcgcgcggc
     7501 gagcggcgag ctggtgctgc gacgcggcta ctgcgagtcc gagccgaccc gctcctcgac
     7561 ctggtaccgg ctgccgcagc actacgacga catggacccg tcgctcgagg cgttccagac
     7621 cggcttctac cagctcacct gggcgcccag gagcttcgac tggatgggcc gcggcttcga
     7681 cctgctgcgc accgatcccc tcgactacgg cagcaccgag cggggtgtga ccagcgagag
     7741 cccgctcttc gacttcgtct tcgaggaccg cgccggcagc gccggccaga ccgcctcgaa
     7801 ggccatcttc ggtgtccgca acctgaacga gcagggccgc tcggatcgct gcgaccggga
     7861 cgagaagctg gtgcgcacgc gcgccgacct cgaggacttc gccgccaaga gctacggcgg
     7921 gtcggtcggc gtgcccggca tcgtctcctt ctcgctgagc aagagccacc aggagatcaa
     7981 cacctcggcc accggcagcg agcgggcctt catcctcgag cgctgcgacg tgcggctgca
     8041 tgcgctgaag atggacctgc gctggcccga ccggcgcggc gaggacagcc tgcgccagcc
     8101 gctcgagacc ggctttcgcc aggcggtcgg gcagcttccg ctggaggcgg gcgacggcaa
     8161 ggcgtactat gccttcctcg acaagtacgg cacgcatttc gccgagcgca tcgtctatgg
     8221 cggcacgttc ttcgccgaga cggaggtgac gaagagcacc taccagatcg ccgagaagga
     8281 gggcgacaag ctctcgctcg cggccgaggg gacgatcaag aaggtgagcc tcggcggcac
     8341 ctatgagaac gagacctcca agggccgcgc caacgacaac ggccactcga gctcgaccta
     8401 ccgcaagtgg tcggtcggcg gcaccggcag cgacgtctat cccgtgtggg cgcccaccgt
     8461 gcccgacaac ccggcgccga tcgatgtcag cttccggccg gtctacgaca tcctgacacc
     8521 ggtgttcttc ccggacgacc cgaatatcgg cctcaagaac gcgctcctgc ggcaggccac
     8581 cgccagctat tttcgccagc acggcaaggg tgacctgacc tcggacccga agggcctcga
     8641 catccgcgat ccgcgctcga tctgcgtcag gatggacgtg gtcgagtaca cctccacctc
     8701 cggccggtcg ggcgacaggt tcgtcagcga tctctcggcc ggcttcgtcg acggcgccag
     8761 gaacgtggtg acggacgccg agccgttcca gctcgtccat gcgccgggca acctgaacga
     8821 cgcgcgcgcg gtcgatccca atctcgatcc cgagcgctgc acgccgccgg tgaccgcgag
     8881 ctacgtccgc tcgggctcga tctacctcca cggcgtcatc ggccagtacc cggacggctg
     8941 ggggcgaccg agcaccacgg ccgagctcag ccggcatttc ccgaaggcgg accagaccta
     9001 cccgctgcgc cggctcgagc cgggcacgcc ccgggtcttc gagggcatga tccagctccc
     9061 cgagggccag gcgcgctaca agctgcgggt tcgggcggtc gatcaggcca cctgacaggc
     9121 tgccgccggc gccggaggga cggggacgcc gcgcggcgca gggacctggt cgcagcctcg
     9181 aagcctgtcc gggaaatggt catgacctgc gcgcgcctac cctggccgag ggcggggaga
     9241 ccggcgcctt cagcttccgt gtccagcgca tcacgtgccg gcccacggca acgagctcgc
     9301 cccgctggtt cgagacctcg atcccggcga tgctccggcc gccggccttg tcgaccgtct
     9361 cgaccacgta atgcacggtg acggtgtcgc ccagcaggac cggccgcagg aagcgtatcc
     9421 ggtcgtagcc tgccgagacc gggaaatccg tcaggtcgtc gtcatggatg acatgggcga
     9481 tgctcatggt cgacgccgtc gacatgtagc cgatgagcag ggcaccatgc gcgatgcgac
     9541 cgccgacgct cgagcgttgc cgcatgtact gctcgttgac atgcgtgtcg ctgaaatccc
     9601 ccgtcaaccc ggcgaacagc atcacgtcgc tctcgcccac cgtccgcgcg aacgtgtagc
     9661 gatcgcccac cttcaccaca tccagccccg acttgcccaa gccacacccc ctttcgactc
     9721 ctgccggcgg cgtcgccgct cggttccagc gggccggctt gcccagcccc tgcccggcgg
     9781 gcatgatccg gccgccagcc cggagaggat agccgcatga cagggaacaa ggtgctcatc
     9841 atcgtcggcg acgccagcga gacggtcgac acgctctatc cctactaccg cctgcaggaa
     9901 gccggcatga cgccggtcgt ggccgggccg gagaagcgcc gcttccagat ggtgctgcac
     9961 gagatccgcc cgggctggac catcacccgc gaatgggaag gctaccagat cgaggccgag
    10021 gtcgccttcg ccgagatcga cccggccgac tatgtcggca tcttcttctc cggcggccgc
    10081 gcacccgaat acatccgcga cgaccccgac ctgatccgca tcacccggca cttcttcgcc
    10141 acgggcaagc cgatcgccag cgtctgccac ggcgtcgaga tcccggcccg cgcggactgc
    10201 gtccggggtc gccgcatggc gacggtggcg aagtgccggt tcgacctcga ggtctgcggc
    10261 ggcatctatg tcgaccagcc ctgcgtggtc gacggcaacc tcgtctccgg ccgcaccttc
    10321 cacgatcacg gccactacat gggcgcctgg atcgagctgc tgcggcagca tgccgtcggc
    10381 cagcggccga aggccgcggc cggtgccgca tgaccgcacg cacggccatc gtcaccggcg
    10441 gccggcgcgg cctcggccgg gccatcgcca tggagctggc gcagagcggc ttcgacgtgg
    10501 cgatcgccga cctcgaccgc gacgatgatg ccaccgaaac catcgccgcc gtcgaggccg
    10561 ccggccggca ggcgcggttc atcgccgtcg acatcgccga tctcgattcg cacgaggccg
    10621 tcctcgccgc ggccgcgagc ctgccggggc cgctctgcac cctcgtcaac aatgccggcg
    10681 tctcgtccat ggtccggggc gacatgctgg agctctcggt cgagagcttc gaccgctcca
    10741 tcgccgtcaa cctgcgcgcc gccttcttcc tgacccagct tttcgccagg cgcctcggtc
    10801 cggccgaccc cgccggcccg ttccgcagca tcgtcaacat cacctcggtc aacgccgccg
    10861 acgtgctggc gccgacccgc gccgactacg gcatgagcaa ggccgcgatg tcgatgatga
    10921 gccgcctctt cgcgctccgc ctcgccgagc tcggcatcca tgtctacgag gtccggcccg
    10981 gcatcatgcg gaccgggatg accgccccct cggccgcgcg ctacgacgcc ttcgtcgcct
    11041 cgggcggcgt gcccatcgcc cgctggggca cgcccgacga cgtcggccgg gccgtcgcca
    11101 tgctcgccaa tggcgggctc ggcttcacca cgggcagcgc catcgaggtc gatggcggga
    11161 tgcacctcca cgcggtctga gccggcgacc cggggaaaag ccccgccttg cccggcaggc
    11221 gtcctctggc atgatccccg catcggacga cgcgggccac atcgccgggg aggccccgga
    11281 ccggtcgaga gcgtcgcccc cgacaacccg atcggatctc gtctctcctg cgcttcctct
    11341 tgcttctcct ctcctccctg ccccgcctcg ccggccgctc ccccgagcgc gccggcgctc
    11401 ttttttggcg ggcgggcgct tccctccgcc ggccccggcg ccagttcatc agcgaaaggg
    11461 gtgccgcctc gccatgaacc tctacgtgcc accgaccatc ccgccgccac gccgcccgct
    11521 cggcatgctc gccagcgtgc gcgccgcccg ccgcaacgtg ctcggcatca tcccggacat
    11581 cgcctacgcc cagccgatcg tgacgggtgc gaccggcccc gcccgctggc acatggtgca
    11641 ggggccggaa gggctgcgcc gcatcttcct cgacaatgtc gagagctacc cgaagtcgga
    11701 gatcatgatc cgcatgctcc gctccgccgt cggcgccagc ctcttcacct ccgagggtgc
    11761 ccagtggcgc tggcagcggc gggccatcgc cccggtcttc accgcgcgca acgtggcggc
    11821 cctggccccg ctcatgacgg ccacggccga gcgcgcctcc ggccgcctcg ccgcctgcgc
    11881 ggggccggcc gagatggtgc gcgagatgct gtccgccacc ttcgacgtga tctgcgaggt
    11941 cgccctctcg ggccgcgagc atttcgacgc cgggctctac ggcgccgcca tcacccgcta
    12001 cttccagacc gtcggccgcg cctcgctcct cgacttcctc gacctgccgc cctgggtgcc
    12061 gcgccccggc gagctcctcg gccggggtgc cgtccggacc atgcaccgca tggtggcggc
    12121 agcgatccgc gcccgccggg cagcaggacc gtcggcgacg gcggacctcc tcgactacat
    12181 gatccgggcg aaggaccccg agagcggccg gacgatgacg ccgcaggacc tcctgcacaa
    12241 catgcagttc ttcatcgtcg ccggccacga gacgacggcc ctcgcgctct cctgggcgct
    12301 gcatctcctg gccaacgacc aggctgccca ggaacgggcc cgagccgagg cccgggcagc
    12361 gctcggcgcc cgcgccgccg gtgcttccga cctcgaggcg atgcccttcg ggcgggccgt
    12421 gctcgaggag gcgatgcggc tctacccgcc ggtcggcatg ctggcgagga acgcgcttcg
    12481 cgaggaccgg ctctacgacc gcgaggtccg cccgaacgac acggtcttcg tcaacatcta
    12541 cgccctgcac cgccaccgcc ggctctggca gaggccggac gccttcgatc cgacccgctt
    12601 cgcgccggaa gcgacgccac ccgaccgcta cgcctatctg cccttcgggg ccggccccag
    12661 ggtctgcgtc ggcgccaatt tcgccatgat gcaggcccag atcatcttct cgaccctgct
    12721 cgcccgcttc cgcttcaccc ccgccggccc gccgccgacc ccggtcatgc acatgaccat
    12781 ccgccccgag ccgggggtca cgctcgaggc gaccccgctg gcggcaggag gagaaaatcg
    12841 ggcggcaaag agcgaaaacg gtttggtgga gccgatcggg atcgaaccga cgacctcctg
    12901 aatgccattc aggcgctctc ccagctgagc tacggccccg tccaccggtg cgcggtaggt
    12961 aggcaaaagg catcggcact gcaagtcaaa tgtcgtcgcc gccggtcggc agggtcaccg
    13021 tggcccgaag gcccccgccc ttgcggttgg cgagggcgac gtcgccgcca tgggcgcgca
    13081 ggatcgtcct ggcgatggcg aggccgaggc cggcgccacc ggtctcgggg ctgcggctgg
    13141 cctcgccccg gacgaaggga tcgaagaccc gctccaggag gtcctcgggc aggccggggc
    13201 cgtcgtcgtc gatggtcagc gtgacgcgct ggccgtcgcc ggccagcgcc agcctggcgc
    13261 tgccgccgta gcggacggcg ttctcgacga ggttgcgcag cgcccgcttc agggcgagcg
    13321 gccggcagcg cagcacgatc cggccgcttt cctggggctc gaccgtcacg gcggcgccga
    13381 gatcggcctg atcggcggcg aggctctcga ccagcgcacc gaggtcgacc gcgaccgtgg
    13441 gctcctcggc cgaggtgccg cgggcgagca gcaggccggc ctcggtcagc gcctgcatct
    13501 cgtccagggt cgccagcatg cgctcctgca attccgggtc gtcgaccatc tcggcgcgca
    13561 ggcggagcgt ggtgatcggc gtgcgcaggt cgtggctgac ggcggcgagc agccgcgtgc
    13621 gctccgccat ggcccggccg atccgcgcct gcatgcggtt gaaggcggtg gccgtgcggc
    13681 gaatctcgac cgggccacgg tcggccggca gctcgaccgg ctcgccgcgg ccgaccgcgt
    13741 cggcggcggc cgccagggcc gccagcgggc gggtgaggcg cctcaggccg agcaccgcgg
    13801 cgaggctgac ggcgagggcg gcggcgacca gcgccacgag cggcgccagg ccgaaggccg
    13861 gccgctcggg cggcagcagc atggcggcat tgagccagct ctcgggcccg acccggatcg
    13921 ccacgagaag gccgggatcg gtcggctgcc tgggctcgac gccggcgtca tgaagctggc
    13981 ggtgccagcg gggcatgccg tggggctgcc ggccgatctc gcccatggcg gtcagcacct
    14041 cgtgctcacc ctcgccctcc aggagatccg cgagcttgcg ggcgaggcgg acggcgggcc
    14101 cctcgccctg ctcctcgacc acggcctcgt cggccagcca gaagcgcagc cggcgggtcg
    14161 agaaggcgcg cagcgcgcgg cgccgctcga cgggctcggt ggtctcgtcg agcaggcggg
    14221 cgagggccgc ggcgcgctcc agcacctgct cgcggccgat cgcctcgacg gtgctctgcc
    14281 gctcgccctg aagaatggcg aaggtggcga gctgggccag gaccagagcg aggagcagga
    14341 tggcgacgag ctggccggtc atgctgcgcg gccagaggcg gctcatgcgg gctccacccg
    14401 cccggtgaag ctgtagccgc cgccccagtg ggtcttgatc agcagcgggt tcctcgggtc
    14461 gcgctcgagc ttcttcctga gccggctcac ctggttgtcg acgctgcggt cgaagggctg
    14521 ggcctcgcgg ccgcgggtca ggtcgaggag ctggtcgcga ctgaggacga ggccggggtg
    14581 gcgcagcagc gccgtgagca ggagatactc gccggtgctg agcggcaccg tcaccccctc
    14641 ctcgtcgacc aggtcgcgcg tgcccggatc gaacagccag cggtcgaagc gcacgcggcc
    14701 ggccgtggcc ggcgcccccg gccggtgcgg caggctctgc gcccggcgca gcaccgcctt
    14761 gatccgggcc aggagctcgc gcggcgcgaa gggcttggtg acgtagtcgt cggcgcccat
    14821 ctcgaggccg acgatccggt cggtctcctc cgccatggcg gtgagcagga tgatcggcag
    14881 gttgccccgc gcgcgcagct cgcggcagag cacgaggccg tcctcgcccg gcatcatgac
    14941 gtcgaggatg acgaggtcga tggcggcccg ggcgagctcg cgccgcgccg cctgggcgct
    15001 gtcggcggtg gtgacgcggt gcccctgctt ctgcaggtac tggcgcagcg catcgcggac
    15061 gtcgcgcgtg tcgtcgacga cgagaagatg gggtccgtcg ctcatgacgg caggctctag
    15121 gccctgccgg ctcccggtgg acagggaaaa atgtcgcgga atgtaacggc cggcgccacc
    15181 cggcacagtc cgtgaccaaa gagcgccgat cgcgacgaag tccagccaaa ggccaggggc
    15241 catatgctcg tcgaacgcga accaccaaag ggaatcgaac gatgagcaga acctcgatcc
    15301 tcgccacggc cgccctcggc ctcgtggcgc tggccggcgg ccaggcgctc gccgatggcg
    15361 gccagggtgg ccggcatatg ggcggcagcg gcctcatggg cggcggccag atgatgcagg
    15421 gcttcgaccg tgacggcgac ggcagcttca cccaggagga tgtcgacgcg gtgcgcggcg
    15481 agcgcttcgc cgaattcgac gccgacggcg acggcgccct ctcgctcgcc gagtacgagg
    15541 cgctctggct cgacgccatg cgcgagcgga tggtgcgcgg cttccagcac cacgaccggg
    15601 acggcgacgc cctggtgacg gtcgaggagt tcaccctgac gaccggcaac ctcgtggagc
    15661 gtcacgatcg caacggcgac ggggtggtca ccagggacga gctccagcga cgcccgcacg
    15721 gcgagggccg gcgcggcggg cagcgccccc gccaggcccc gccggcgggc gacagcccca
    15781 actagccagg aagacggagg ccggggcggg cggcgctcag cgccgctcct cctcgccgtc
    15841 ggcatccttg tcgatgacgt cgccgagcgc gtcgtcgtcg ccgagttcgg acgcgtcctc
    15901 gatcagcgcg tcctcctcct cgtcgtcctc ggtctcgacg acctcgggct cgtccgcctc
    15961 ttcctcgtcg gtgtcgtcga cgtcgtcctc gacctcctcg acatcgtcct cggtgtcgtc
    16021 ggtctcggcc tcgtcgacct cctcctcgac ggcggcggcg gccggcgccg cccgggtcac
    16081 ggtgcgcggc cgtctggcgc gggcgaggag ctccagatcg aacaccgcgc cacaggccgg
    16141 gcaggccagg tcggcggtct tgccgaagtc gtagaagcgg gcaccgcagg cgaggcaagt
    16201 gcgcttggtt ccaagttccg gcttgggcaa ggcggtcccc agtcatgctg acaggatgaa
    16261 gggcgacgcc cctcgcatgg ggcgccctga agcgggcaag gcaattgcca tgatcgcccg
    16321 gggctgtcaa aagcaaatcg cgcgaggcgt tggcggctct gccgccggct ctccggggca
    16381 tcgccggagc gcccgcgagc ccggtcggcc gggctcggct cgcgccccgt ccagccagcg
    16441 atttcgagga ctcgacgatg ctgctccagg cggccccaca cggcgccctc cagggtgacg
    16501 ccgtggttcc cggcgacaag tcgatgtcgc accggtcgct cctgctcgcc gccctcgccg
    16561 tgggcgagag ccgcatcacc ggcctgctcg agggcgccga cgtcatggcg acggcgggtg
    16621 cgctgcgcgc cctcggcgcc acggtcgagc ggcagccgga cggcacctgg acggtgcggg
    16681 gctgcggcgt cgccgggctc gccgagccgg ccgacgtgct cgacctcggc aatgccggca
    16741 cgggtgcgcg gctgctcatg gggctgctcg ccgggcacgc cttcaccacc ttcctctccg
    16801 gcgacgcctc gctgcgctcg cggccgatgc gccgcgtcac cgagccgctc gtcgccatgg
    16861 gggcgtccgt gctcggccgc agcggcggcc ggctgccgct cgccatcacc ggccgcaacg
    16921 acctcgtgcc gatcacctgg cgctcgccgg tcgcctcggc ccagatcaag tcggccgtgc
    16981 tgctcgccgg cctgcacgcg ccggggcgga cgaccgtcgt cgagcccctg ccgtcgcgcg
    17041 accataccga gcgcatgctg gcggccatgg gcgcaaagat aaccggcgag gccggcgccg
    17101 acggcggctg gacggtggcg atcgagggcg aggccgagct cgcgccgcag gccttcctgg
    17161 tgccggccga cccctcgtcg gccgcctttc cgctggtggc ggccctgaca gcaccgggct
    17221 cggcgctcac cttgccgggc atctccgtca acccgctgcg caccgggctc ctcgccacgc
    17281 tccgcgagat gggagcgaag ctggagctcg cgaacgagcg cagcgtcgcc ggcgagcccg
    17341 tggccgacct cgaggtcagg gcgagccggc tcgtcggtgt ggacgtcccg gcggcgcgcg
    17401 caccctcgat gatcgacgag tacccgatcc tcgccgtggc cgccgccttc gcccaaggca
    17461 ccacccggat gcgcggcctc gccgagcttc gggtcaagga gagcgaccgc ctcctcctcg
    17521 tggccgaggg cctcgccgcc tgcggcgtcg aggtcgccgt cgagggcgac gacctcctcg
    17581 tcaccgggcc ggtccgcgcc acgcgggcgg cccggatcga cgcccaccac gaccatcgga
    17641 tcgccatgag cttcctcgtc atgggcggcc tcggctcggc cccggtgacg gtggccgggg
    17701 ccgagaccat cgagaccagc ttccccggct tcgccgacct catgaaccgg ctcggcgccg
    17761 ggctcgaggc gatcgagccg tgagccgggc ggtcatcgtc gccatcgacg gcccggcggg
    17821 ctccggcaag agcacgctgg cgaagcggct ggccgccacc acggggctgc ggttcctcga
    17881 caccggcctg ctctaccggg cggtcgcggc ccggctccgc gccaccggcg gctcacccct
    17941 ggacgcggcg gcggcggcgg cggcggcgcg caccatcgag cccgaggggc tcgaccccga
    18001 cgccctgcac ggcgagggga tcggcggcga tgcctcccgg gtggccgccc tgcccgaggt
    18061 gcgcgcggcc ctgctcgagc tgcagcggcg cttcgcccgg accccgccgg gtgcggtgct
    18121 cgccggccgc gacatcggca cggtcgtctg ccccgacgcc accctcaaga tcttcgtcac
    18181 cgcctcgatc gaggaacgcg cccggcgccg cttcgaggag ttgcgggcac gcggcgaagc
    18241 gcctatacac gagcgcgttt tattggaggt ggccgagcgt gatcgccgcg accgagagcg
    18301 ggcggtcgcg cccttgtccg ttgccgaaga tgcggtggtg atcgacacct cgacgctcga
    18361 cgtcgagcag tcgcacgcag cgctcgaggc agcgtatgca gcacggatcg ggtccagcgc
    18421 caatcgaaca tcgtagtcaa ccctggcccc gcccggctcc ggccggcggt tttggagcaa
    18481 gaatgcagga agccatcgcg accacccaag ccccgtccca ggccgat
//
LOCUS       DAOTFI010000084        18472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3614_length_18472_cov_6.597817, whole genome shotgun sequence.
ACCESSION   DAOTFI010000084 DAOTFI010000000
VERSION     DAOTFI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18472
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3614_length_18472_cov_6.597817"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="P5558_10330"
     CDS             complement(<1..633)
                     /locus_tag="P5558_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY24748.1"
                     /translation="MAAGATFDVRRDLSGIVASLHTPFTADDRLDELRLARLVDHVAR
                     AGCTGVLASAVAGEVGSLRPDERQRLLEVVGDACRGRLRVVAGVSAAEPGTSLALARS
                     AAALGADMVLWQPPAGLAGPALQDALARLGEVAPVMLQDLDWHGGGLALGTIVDLAEA
                     VPGFEALKIETAPAGPKYTAVRDALGDRLHLSGGWAAMQMLDGLARGLDAF"
     gene            complement(638..1294)
                     /locus_tag="P5558_10335"
     CDS             complement(638..1294)
                     /locus_tag="P5558_10335"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY24749.1"
                     /translation="MTQLEAHLAQNRLPEIARLPSGRALRETLRVTRGERRKSLAVLE
                     AEGAVWRRVGKGTLLGARPVQGLSTVGGSADLTNPREVMRARLLLEPVLAGEAALNAT
                     GSDLDEMELCVVAARRVETWRHYENWDNRLHRAVAEAAGNAALLALLDLLNAVRRAVV
                     WGRQGSKPERPSEDHHSLADHEAVVAAIRERDVVGAAEAMRAHLGKVERNLIAVGSAA
                     "
     gene            1761..2639
                     /locus_tag="P5558_10340"
     CDS             1761..2639
                     /locus_tag="P5558_10340"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24750.1"
                     /translation="MNERIGVVGLGRMGLAMAETLARTGYDVTGHDVMAPARERARAA
                     GIAVEERLGALLKGSGLVLSSLPRDADVEAIVEGPEGLLAQAPGRLLVDTSTVAPPTT
                     RRLAPLLADHGHGLIDAPVSGGPAGAATGALTFMVGGSAAHVARARPLLDALGKAVVH
                     VGDSGAGNLAKLVNNLFCATHLVGVGEALRLAEALAVDPARLFEAVNAASGRSAVSEV
                     NLPRWILSGSFDSGFTMGLMRKDVRLAAALAPAAGLSLPLARAVCELWQASAATLPDD
                     ADFNRMTQFAPANDHD"
     gene            2632..4152
                     /locus_tag="P5558_10345"
     CDS             2632..4152
                     /locus_tag="P5558_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY24751.1"
                     /translation="MTERSTRRLASLKSFALDAGLGSHVGGGLVAGRGETVELVDPCD
                     GETWTIYPDAGEAVVDQAMQAASGALTTWQALTASARGRLVWQVGQKVRERLDDLAQL
                     ETLTTGKPIRDTRAEVLKVAEMFEYYAGWCDKLHGQVIPVPTSHLNYTRREPLGVVVQ
                     ITPWNAPIFTAGWQIAPAIAAGNAVVIKPSELTPASTLALARLAEEAGLPKGLVNVLA
                     GLGQTTGEAAVTHAETRLVVFVGSPRTGALIAAAAARNTVPTILELGGKSANIVFADA
                     DLDRAALGAQAAIWSGAGQSCVAGSRLVVDRRVQEQLLDRLATAAGRLVVGAPEDEAT
                     AIGPILCERQWRTVDGMVEAARQAGARVRAGGGRPAHLAERGCYFAPTLIDEVRPDMA
                     IARDEVFGPVLTVLPFDDEEEAIAIANGTRFGLAGAVWTQDVGRAHRVAAAVRAGTFW
                     VNGYKTISVMSPFGGFGASGHGRSSGEEALLAYTQTKSVWIETAAEPPAGFGYGGD"
     gene            4238..5422
                     /locus_tag="P5558_10350"
     CDS             4238..5422
                     /locus_tag="P5558_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HRY24752.1"
                     /translation="MTFDLVLKGGHVIDPAQGIDRITDVAFEGGKVAAIGDGLDGRDV
                     RHVDGLIVTPGLIDLHTHVYWGGTSLGVDAEDFCRRSGVTTAVDTGSAGPGNFPGFRK
                     HVIEPSQTRILAYLHISFAGIYAFSNTVMVGESEEMRMMAPAEAAAVADANRDVIVGI
                     KVRVGRHASGTSGVAPLDMALQVADAVGMPLMAHIDHPPPTYEEVIERLRPGDVLTHA
                     FRPFPNSPVDGKGQVKDLVWRAREKGVIFDIGHGKGSFAYKTARAMLEGGFRPDVISS
                     DVHTLCIDGPAYDQVTTMSKFLCLGMPLGEVIAASTVNAAKALQRPELGSLAPGSVGD
                     ATLLELKDGSFRYEDVTGEIMTGDRRILARGAVVGGRVFHPAEPDAFRASASHSSTRA
                     AE"
     gene            5419..5886
                     /locus_tag="P5558_10355"
     CDS             5419..5886
                     /locus_tag="P5558_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY24753.1"
                     /translation="MTEKTADQRLAELGLTLPTAPTPIYTYVPFKRDGSTVYLSGQGP
                     KLPDGTYAQGKVGKDVTVEEAAEHARLTGLYLLAVAKLAAGSLDKVEFLKLFGMVNAV
                     PDFKDQPTVINGCSNLLVDVLGERGRHARSAIGMGSLPNGMTVEIEAVIKIHE"
     gene            complement(5899..6873)
                     /locus_tag="P5558_10360"
     CDS             complement(5899..6873)
                     /locus_tag="P5558_10360"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRY24754.1"
                     /translation="MTATPPALEVSGLKKHFPVQKGLLKRTVAQVKAVDGVSFAIQPG
                     ETLCLVGESGCGKSTVAKVALRLIEPTAGSIKLDGVEVSQLDAEAMRPFRKRIQIVFQ
                     DPYASLNPRMTAGRIVSEPLENYSDISRAEREERMALLFEKVGLRRDSMNRYPFEFSG
                     GQRQRLGIARALALEPKVIVADEPVSALDVSVQAQVLNLLMDLQEEFGLSYLFVSHDL
                     GVVEHIGHRIAVMYLGKIVELATREALFANPLHPYSRALISAAPMPNPRAKRERIVLE
                     GDVPSPMNPPSGCAFHTRCPFAIPRCRSETPALEPKPDGRLVACHLVE"
     gene            complement(6870..7871)
                     /locus_tag="P5558_10365"
     CDS             complement(6870..7871)
                     /locus_tag="P5558_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24755.1"
                     /translation="MTQPLLSVRDLETHFFTADGVTRAVGGVSFDVMAGETVGIVGES
                     GCGKSVTALSIMRLLPDRLARTVAGSVRFEGTDLLALDEARMRDIRGNRVAMIFQEPM
                     TSLNPVLTIGDQIAEAVRIHQGAGTEEANARAVEMLKLVRIPDAERRLGDYPHQFSGG
                     MRQRVMIAMALACNPKLLIADEPTTALDVTIQAQILNLMIELKARTGAAVILITHDLG
                     VVAETCQRVIVMYAGRKIEESGVIELFDRPAHPYTRGLMTSIPSFGGRGRQRRLTEIP
                     GIVPSLREPITGCAFAPRCPFAVERCLSEAPELRPVGEGHIAACHEAERVLATELVA"
     gene            complement(7882..8673)
                     /locus_tag="P5558_10370"
     CDS             complement(7882..8673)
                     /locus_tag="P5558_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24756.1"
                     /translation="MALGGLILILLLVTAVAAPYFAGDPISMTPAQRLKPPSAEFWFG
                     TDHLGRDVFARTVYGARISLLVGLSVAIFSIVVGLAIGLLAGYFRKVDAVVMRLMDGL
                     MAIPAILLAIALVALTKASVFIVIVAITIPEVPRVVRLVRAVVLSVREAPYVEAAVAS
                     GTRTLRIIRRHILPNTIAPLIVQSTYICASAILVEAALSFLGAGTPPDVPTWGNMIAQ
                     SRLYLSVAPWTIFCPGIALATVVLAVNLLGDGLRDRLDPRLARRM"
     gene            complement(8766..9707)
                     /locus_tag="P5558_10375"
     CDS             complement(8766..9707)
                     /locus_tag="P5558_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24757.1"
                     /translation="MLGYIIRRLGSTVLVMGIVGVFVFLLLHLSPGDPAAIVAGDNAT
                     PEQIAQMRERLGLDDPLPVQFIRWSGGVLQGDLGISIFSNEPVAKLIGERLEPTISLS
                     LTTIVFAVVIAVSFGVLAAWRAGTLIDRALMGFSVLGFSVPVFVVGYLLIYLFSINLR
                     WLPVQGYAPIADGIGPWIQRLILPTIALGLAYVALIARITRTSMLEVLSEDYIRTARA
                     KGVATRPMLLKHALKNAGVPIVTVIGIGVALLIGGVVITETVFNIPGVGRLVVDAIAK
                     RDYPIIQGVILLFSGVYVLVNLLVDLSYSFLDPRIRY"
     gene            complement(9770..11386)
                     /locus_tag="P5558_10380"
     CDS             complement(9770..11386)
                     /locus_tag="P5558_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24758.1"
                     /translation="MRTTRRTFVHGSMATAAASTALTGLPWQRAMAQESGGTIKAVMQ
                     GDLRAFDPIWTTANITAYHGAMIYDTLFALDEDMKPQPQMVDTWTVSDDQLTYEFVLR
                     DGLAFSDGTPVTSADVVASIRRWAVRDGAGQHMMLRVKDIVARDDKTFAIELNEPYGL
                     VIDIMAKTSTPLLYIMREQEAMTDPMEQVTEYIGSGPFIFNREETRQGAQYVYDKNPN
                     YVPRSEPASGMAGGKVVKVDRVIFENIDDSQTAMAALQAGEIDFMELPPIDLLSVLEA
                     DPNIVIDVLNNTGNYGLMRLNFLYPPFDNVKARQAMLYLIDQEEFMQATFGDPRYYRK
                     CPSNFACGTPMENDANTEWFAAAPDPERAKQLFEEAGYDGRPVIVLHATNIDFMNNAA
                     QIVAQRLRDIGVNAELATSDWGGVVTRRAVQDPPEDGGWNIFITWAGAASANNPIALA
                     AHAANGTKGWFGWPTDEKHEELRDEWAAAQGLEAQQEIARQIQENAWNFVPHVWLGQW
                     VSPVAYRSNLEGILKIPEIIPFWNIERKAS"
     gene            complement(11505..12983)
                     /locus_tag="P5558_10385"
     CDS             complement(11505..12983)
                     /locus_tag="P5558_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRY24759.1"
                     /translation="MRLFTASLATESNSFSPIPTDRRSFEECVYFRPGEHPDRPTHST
                     ASLWVARNRAAQEGFELIEGSCFWAEPSAPVVQADYESMRDEILAQLEAALPVDGVLL
                     GLHGAMLAQGCDDCEGELIERVRELVGPAAVIGVAFDPHCHLTEKRVRNANVIILFKE
                     YPHTDFVERAEELVSLVTRTIRGEIHPVMSLYDCRMDDLFPTTREPARSFVAKMKVHE
                     ARPEMLSVSLAHGFTQGDVADQGVRVLVVTDDAKAKGDALALELGDEVRARRGTWRPP
                     HCSIDEAIDLAYAAEAGPVVVADPSDNAGGGAASDNTNVLRRLLERGCGDAAIGPFWD
                     PFAVAICHTKEIGSTFPLRFGGKASAASGAPVDAEVTLIGLKRDGWQSFGTAKVSFGD
                     AAGIRIATAGGSVDVTLISNRTQALGTEIFSDVGIDLASKRYIGVKSTHHFHAAYAPI
                     ASEVIYCDGLGPSPLDSRLYPYRKISRPHWPHDELPPGRFLV"
     gene            13064..13816
                     /locus_tag="P5558_10390"
     CDS             13064..13816
                     /locus_tag="P5558_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRY24760.1"
                     /translation="MEVEWRHLTAAALNERAGAGAIVLLPVASTEQHGPHMATGTDAL
                     LVAEVCRRVALRIDGVVAPTLWVGLAEHHMSFGGSFTLRLSTYQALLADLLVSIRRAG
                     FGRVVIVNGHGGNMTALNAMSAELSLGSGLELAVTTYFLLAEAAMAPHLEDQPGVLHA
                     GEAETSMMLALRPESVDTARLAEATGPMPERGSALASPLHRWRSFAELSTTGVIGDAR
                     RATALKGEHLLEAAATALAEQLAAGEPWAQEQ"
     gene            13853..14938
                     /locus_tag="P5558_10395"
     CDS             13853..14938
                     /locus_tag="P5558_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HRY24761.1"
                     /translation="MTSPLAAAIARQYGTPAVVIDLDIVERNIDRLQQACDVAGLRNR
                     PHIKTHKSPVLAALQRDRGARGITCQKLGEAEVMAAAGLDDILISYNLIGEEKMQRLG
                     RLLRTGVTVRPCCDNPVAADGYVDAARLAGRELEVLVECDTGRERAGVETPAEAIALA
                     RRIAGHDGLRFGGLLLYAPEDRQAESQRFLDEVKAGLRENGLEAGIVSTGGTPNLRNL
                     GRIEGATEHRSGTSIFNDRMMMACGHATLEDCALAVYATVVSRAGPDRGILDSGSKTL
                     TSDTGGGLDGHGLIREYPKARIKGFAEEHGMLDLSACDPKPGIGETVSIIPNHVCVVV
                     NMVDELVTVRGGEITGTLPVAARGKLR"
     gene            15091..15771
                     /locus_tag="P5558_10400"
     CDS             15091..15771
                     /locus_tag="P5558_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24762.1"
                     /translation="MPTDVVGKLFAKLADPANHSAAIIEAALNLAHADGLSKAVDLDP
                     WLERFVEASIRELEGRYMGLFSLFWTDDAIGREEFAQYVTSCFEPPSSRELTWPWPIT
                     EPGPSKGGSPGIFEHEHSGLWLCGYRTGKTKGLPDNERKLFLAYFFRQPLPPIVAQYH
                     DDDYGAPGTEKRLRKMANLMAACCRNFKSRDRRKLRVAIAHYEEDLEFLRSNFYRPGM
                     FPWPPIEP"
     gene            complement(15794..16732)
                     /locus_tag="P5558_10405"
     CDS             complement(15794..16732)
                     /locus_tag="P5558_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRY24763.1"
                     /translation="MLSRTASNLFWLARSVERMDYVARLLDVARHMSVLASPNSLNEW
                     HSALIAAGCETPFFELHERSDEAAVLHFLIRDRRNPSSIMSCLVQARNNARAVRAALT
                     LDVWEAINGAYLEAREFDEETFSSDNFSTFVDWAKRQALLFNGAYTNTLLRTENFNFL
                     RLGTFLERADNTARILDVKYHVLLPSFEQVGGSLDYYQWAAILRSVSARRSYHALYKG
                     RVVPWQVVEMLILRPEMPRSLRACYEQVLENLDTLAQRHGGRAGEAHRLAGEMHARLR
                     YSRIDDIFQRGLHEFLTDTIDRTVVIGGEIQKFYLG"
     gene            complement(16737..18212)
                     /locus_tag="P5558_10410"
     CDS             complement(16737..18212)
                     /locus_tag="P5558_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRY24764.1"
                     /translation="MFDEMHGLDASCRPAYAVVEDWLSRTPPDFLARKEREAEALFRK
                     IGITFAVYTEGGDPERLIPFDIIPRVLDAGEWALLASGLEQRVKALNRFVQDMYHGRE
                     IIRAGTVPERLVEGNPCFERKMVGVEVPAGVYTHIAGIDMVRTGPDEFYVLEDNCRTP
                     SGVSYMLENREVMMRLFPDLFARQAIQPVSHYPEELLATLQSVAPPNCPGDPVVALLT
                     PGFYNSAYYEHSFLADEMGVELVEGQDLVVQNGVVMMRTTEGPKRVDVIYRRIDDAFL
                     DPLAFNPDSALGVPGLFDAYCKGNVTLANAVGSGICDDKAIYTYVPDMVRFYLGETPI
                     LNNVPTWRCAEADDLAYVLDHMAELVIKAVHGSGGYGMLVGPHASKAEIQEFRAKLQA
                     KPHDYIAQPTLALSTCPTFVANGVAPRHVDLRPFVLLGGDAVRIAPGGLTRVALKEGS
                     LVVNSSQGGGTKDTWVLAPTAQAASQSQSQSQVTRQTVGEG"
     gene            complement(18351..>18472)
                     /locus_tag="P5558_10415"
     CDS             complement(18351..>18472)
                     /locus_tag="P5558_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY24765.1"
                     /translation="AVTTHTLETHVYRLRQKIEPIAGENTLLLTEPGGYRLVR"
BASE COUNT         2966 a         6264 c         6398 g         2844 t
ORIGIN      
        1 gaaggcgtcg aggccccgcg ccagcccgtc gagcatctgc atggcggccc agccgccgga
       61 gagatgcaac cggtcgccga gggcgtcgcg cacggcggta tatttcgggc cggccggtgc
      121 cgtctcgatc ttgagcgcct caaagccggg caccgcttcg gctaggtcga cgatcgttcc
      181 gagcgccagc cctccgccgt gccagtcgag gtcctgcagc atcaccggcg ccacctcgcc
      241 gagccgtgcg agcgcgtcct gcagggctgg cccggcaagc cctgccggcg gctgccagag
      301 caccatgtcg gcaccgagcg cggccgccga gcgggcgagc gccaggcttg tcccgggctc
      361 ggcggcgctg accccggcca ccacccggag ccggccgcgg caggcgtcgc ccaccacctc
      421 gagcagcctc tgccgctcgt ccggccggag cgatccgacc tcgcccgcga cggcactcgc
      481 cagcacgccc gtgcagccgg cccgggcgac gtgatcgacg agtcgggcga ggcgaagctc
      541 gtcgagccgg tcatcggcgg tgaagggcgt gtgcaggctg gcgacaatac cggagagatc
      601 gcgtcgaacg tcgaatgttg ccccggccgc catttcgtca ggccgctgaa ccaacggcga
      661 tgagattgcg ctcgaccttg ccgagatgcg cccgcatggc ctcagctgcg ccgacgacgt
      721 cccgctcgcg aattgccgcc acaacggcct cgtgatcggc gaggctgtga tggtcttccg
      781 acggccgctc gggcttcgag ccctgccggc cccagacgac ggcgcgacgg accgcgttca
      841 ggagatcgag gagggcaagg agcgcggcat tgccggccgc ctcggcgacc gcccgatgca
      901 gccggttgtc ccagttctcg tagtgacgcc aggtttcgac ccgccgggcg gcgacgacgc
      961 agagctccat ttcgtcgaga tcggagcctg tcgcgttgag cgctgcttca ccggcgagaa
     1021 ccggctcgag caggagacgg gcgcgcatca cctcgcgcgg gttggtcaga tcggcgctgc
     1081 cgccgacggt cgagagcccc tgcaccggcc gggcaccgag aagggtaccc ttgccgacac
     1141 gccgccagac tgcgccttcg gcctcgagaa cggcgagcga cttgcgccgc tcgccgcgcg
     1201 tcacgcggag ggtctcgcgg agcgcccgcc cggatggcaa cctcgcgatt tccggcagcc
     1261 ggttctgggc cagatgagcc tcgagctggg tcaacgcgcc ctgtccctcg tccattttcc
     1321 gcctgccgcg ctccacttgg ccaatattct tctgggcgag gtcgaccccg tcaagcggta
     1381 tggcgagtgg acagaagact attgtcgaag atccggttag ccatcgtcga gggtgagcgt
     1441 gccgcgcgct gcccgctcgt cgattttgcg ttccacacaa agaccgggca attgggaggc
     1501 ccggcagcgg agttacggat cgagggctct tgggacctcg agccgctgca gcgcgcggca
     1561 aacatattcc aaacacaacg cgcgtggccg acctttcggg cgggcggggg caaccccgcc
     1621 cgtttctctc cctgaccctc tccgcacgga tcgagggctt gcgcaaggca agcccgaggg
     1681 tcgtccgacc ccgtcattgc cggcggtccg ccgatggggt tagatcaccg ccatcggaaa
     1741 ccgtcggtgt cggacacagg atgaacgagc gcattggcgt cgtgggcctc ggccgcatgg
     1801 ggctcgccat ggccgagacc ctcgccagga ctgggtatga cgtcaccggc cacgacgtca
     1861 tggcaccggc ccgcgagcgc gcccgggctg ccggcatcgc ggtcgaggag aggctcggcg
     1921 cgctgctcaa aggtagtggg ctggtcctca gttccctgcc gcgcgacgcg gatgtcgaag
     1981 cgatcgtcga gggacccgag ggcctgctgg cgcaggcgcc gggccgcctc ctcgtcgaca
     2041 cctcgaccgt cgccccgccc accacccgcc ggctggcgcc gctgctcgcc gaccatggcc
     2101 acggcctcat cgacgccccg gtgagcggcg ggccggccgg ggccgccacg ggcgccctca
     2161 ccttcatggt cggcggcagt gccgcgcacg tggcgcgtgc ccgccccctc ctcgatgccc
     2221 tcggcaaggc cgtcgtgcat gtcggcgaca gcggtgcggg caacctcgcc aagctggtca
     2281 acaacctctt ctgcgccacc catctggtcg gggtcggcga ggcgctgcgt ctcgccgagg
     2341 cgttggcggt cgatccggcc cggctgttcg aggcggtcaa cgccgcctcg ggccgcagcg
     2401 ccgtttccga ggtcaacctg ccgcgctgga tcctctccgg cagcttcgac agcgggttca
     2461 ccatgggctt gatgaggaag gacgtgcggc tcgccgcggc gctcgctccg gcagccgggc
     2521 tgagcctgcc cctggcccgc gcggtctgcg agctctggca ggcgagcgcc gcgaccctgc
     2581 ccgacgatgc ggacttcaat cgcatgaccc agttcgcccc ggccaacgac catgactgag
     2641 cgcagcacca gacgcctggc ttcgttgaag tcgttcgccc tcgatgccgg gctcggcagc
     2701 catgtcggcg gcgggctggt ggcagggcgc ggcgagacgg tcgagctggt cgatccctgc
     2761 gacggcgaga cctggacgat ctatccggat gccggcgagg cggtggtcga ccaggcgatg
     2821 caggccgcct ccggcgccct gacgacatgg caggcgctca ccgcctcggc ccggggccgg
     2881 ctcgtgtggc aggtgggcca gaaggtccgc gagcgcctgg acgacctcgc ccagctcgag
     2941 acattgacca ccggcaagcc gatccgcgac accagggccg aggtgctcaa ggtcgcggag
     3001 atgttcgagt attacgccgg ctggtgcgac aagctgcacg gccaggtcat ccccgtgccc
     3061 accagccacc tcaactacac ccgccgcgag cccttgggcg tggttgtcca gatcaccccc
     3121 tggaacgcgc cgatcttcac cgccggctgg cagatcgccc cggccatcgc cgccggcaac
     3181 gcggtcgtca tcaagccatc ggagctgacc ccggcgagca cgctggcgct ggcccggctc
     3241 gccgaggaag cggggctgcc gaaggggctg gtgaatgtcc tggcggggct cggccagacc
     3301 accggcgagg ccgcggtcac ccatgcagag acccggctcg tggtcttcgt cggctcgccg
     3361 cggaccggtg cgctgatcgc cgcggccgcg gcccgcaaca ccgtgccgac gatcctcgag
     3421 ctcggtggca agtcggccaa catcgtcttc gccgacgccg atctcgaccg tgccgcactc
     3481 ggtgcccagg cggcgatctg gagcggggcc ggccagagct gcgtcgcggg ctcgcggctg
     3541 gtcgtcgatc ggcgcgtcca ggagcagctt ctggaccggc tcgccacggc cgccggccgc
     3601 ctcgtcgtcg gtgcgccaga ggacgaggcg acggcgatcg ggccgatcct ctgtgagcgg
     3661 cagtggcgga ccgtcgacgg catggtcgag gcagcgcggc aggcaggtgc tcgggtgcgc
     3721 gccggtggcg gccggccggc gcatctcgcc gagcggggct gctacttcgc gccgaccctg
     3781 atcgacgagg tgcggccgga catggccatc gcccgggacg aggtcttcgg gcccgtgctc
     3841 acggtgctgc ccttcgacga cgaggaagag gccatcgcca tcgccaacgg cacccgtttc
     3901 ggcctcgccg gtgccgtctg gacgcaggat gtcggccggg cgcatcgcgt cgctgccgcc
     3961 gtccgcgccg gcacgttctg ggtcaacggc tacaagacga tcagcgtgat gtcgcccttc
     4021 ggcggcttcg gcgccagcgg ccatggccgc tccagcggcg aggaagcgct cctcgcctac
     4081 acgcagacca agagtgtctg gatcgagacc gccgccgagc cgccggcagg cttcggctac
     4141 ggcggcgatt gaggcggact tcgctcgttc ggcactggac cctgggccgg cttggcggca
     4201 ataaggccct ctggacgttg tggaggggga atacgccatg acttttgacc tcgtgctcaa
     4261 gggcgggcac gtgatcgatc cggcccaggg catcgaccgg atcaccgacg tcgccttcga
     4321 gggcggcaag gtcgccgcca tcggcgacgg tctcgacggc cgggatgtcc gccatgtcga
     4381 tggcctgatc gtcacccccg gcctgatcga cctccacacc cacgtctact ggggcggcac
     4441 ctcgctcggc gtcgacgccg aggacttctg ccgcaggagc ggggtcacca ccgccgtcga
     4501 cacgggtagc gccggccccg gcaatttccc cggattccgc aagcatgtga tcgagccgag
     4561 ccagacgcgc atcctggcat atctgcacat ctccttcgcc ggcatctacg ccttctcgaa
     4621 caccgtgatg gtcggcgaga gcgaggagat gcggatgatg gcgccggccg aggcggccgc
     4681 cgtggcggac gccaaccgcg atgtcatcgt cggcatcaag gtccgggtcg gccgccacgc
     4741 ttccggcacg tccggcgtgg cgccgctcga catggcgctg caggtggcgg atgcggtggg
     4801 catgccgctc atggcgcata tcgaccaccc gccgccgacc tatgaggagg tgatcgagcg
     4861 gctgcggccg ggcgacgtgc tgacccatgc gtttcgcccc ttccccaaca gcccggtcga
     4921 cggcaagggc caggtcaagg atctcgtctg gcgggcccgc gagaagggtg tcatcttcga
     4981 tatcggccac ggcaagggct ccttcgccta caagaccgcg cgcgccatgc tggagggtgg
     5041 cttccggccc gacgtcatct cgtccgacgt ccataccctc tgcatcgacg gcccggccta
     5101 cgaccaggtg acgacgatgt cgaagttcct ctgcctcggc atgccgctcg gcgaggtgat
     5161 cgccgcctcg acggtgaatg cggccaaggc cctgcagcgc cccgagctcg gcagcctggc
     5221 gccgggcagc gtcggcgacg cgaccctgct cgagctcaag gacggctcgt tccgttacga
     5281 ggacgtgacc ggcgagatca tgaccggcga ccggcgcatc ctcgcccggg gtgccgtcgt
     5341 cggtggccgg gtcttccatc cggccgagcc cgacgccttc cgcgccagcg ccagccacag
     5401 cagcacgagg gccgccgaat gaccgagaag accgccgacc agcgtctcgc cgagctcggc
     5461 ctgaccctgc cgaccgcccc caccccgatc tacacctacg tgccgttcaa gcgcgacggc
     5521 tcgaccgtct acctctcggg ccagggcccg aagctgccgg acgggaccta cgcccagggc
     5581 aaggtcggca aggacgtcac cgtcgaggag gccgccgagc acgccaggct caccggcctc
     5641 tacctcctcg ccgtcgccaa gctcgccgcc ggcagcctcg acaaggtgga gttcctcaag
     5701 ctcttcggca tggtcaacgc cgtgccggac ttcaaggacc agcccaccgt catcaatggc
     5761 tgctccaacc tgctcgtcga cgtgctcggc gagcgcggcc gccacgcccg ctccgccatc
     5821 ggcatgggct cgctgcccaa cggcatgacc gtcgagatcg aggcggtgat caagatccac
     5881 gagtaggcgg gccgctgcct actccaccag gtggcaggcc accagccggc cgtcgggctt
     5941 gggctcgagg gccggcgtct cgctgcggca gcggggaatc gcgaaggggc agcgggtgtg
     6001 gaaggcgcag ccgctgggcg ggttcatcgg tgacgggacg tcgccttcga gcacgatgcg
     6061 ctcgcgtttg gcgcgcgggt tcggcatggg tgcggcggag atcagggcgc gggaataggg
     6121 gtgcagcgga ttggcgaaga gcgcctcccg cgtcgccagc tcgacgatct tgccgagata
     6181 catgaccgct atgcggtggc cgatatgctc gaccacgccg aggtcgtggc tgacgaagag
     6241 ataggagagg ccgaactcct cctgcagatc catgagcagg ttcaggacct gcgcctgtac
     6301 cgagacgtcg agtgcggaaa cgggttcgtc ggcgacgatc accttgggct cgagcgccag
     6361 ggcccgggcg atgccgagcc tttggcgctg cccgccggag aactcgaagg ggtagcggtt
     6421 catcgaatcg cgccggagcc ccaccttctc gaacagcagc gccatgcgct cctcgcgctc
     6481 ggcacgcgaa atgtcgctgt agttctcgag cggctcgctg acgatccgcc cggcggtcat
     6541 gcgcgggttg agcgaggcgt aggggtcctg gaagacgatc tggatgcgct tgcggaaggg
     6601 ccgcatggcc tcggcgtcga gctggctcac ctcgacgccg tcgagcttga tggagccggc
     6661 cgtgggctcg atcagccgga gcgccacctt ggcgaccgtc gacttgccgc aacccgattc
     6721 gccgacgagg cagagcgtct cgcccggctg gatggcgaag ctcacgccgt ccacggcctt
     6781 gacctgggcc acggtccgct tgagcaggcc cttctgcacc ggaaagtgct tcttcaggcc
     6841 agaaacttcg agagcgggag gtgtcgcggt catgccacga gctccgtcgc cagcacccgc
     6901 tcggcctcgt ggcaggcggc gatgtggccc tcgcccaccg ggcggagctc cggcgcctcg
     6961 ctcaggcagc gctcgaccgc gaaggggcag cgcggggcga aggcgcagcc ggtgatcggc
     7021 tcgcgcaacg agggcacgat gcccgggatc tcggtcagcc ggcgctggcg gccgcggccg
     7081 ccgaagctcg ggatcgaggt catgagcccg cgcgtgtagg gatgcgccgg ccggtcgaag
     7141 agctcgatca ccccggactc ctcgatcttc cgcccggcat acatgacgat cacccgctgg
     7201 caggtctcgg ccaccacacc gagatcgtgg gtgatcagga tcaccgcagc gccagtgcgc
     7261 gccttgagct cgatcatcag gttgaggatc tgcgcctgga tggtgacgtc gagagcggtg
     7321 gtcggctcgt cggcgatgag cagcttcggg ttgcaggcga gcgccatggc gatcatcacc
     7381 cgctggcgca tcccacccga gaactggtgc gggtagtcgc cgagccgccg ctcggcatcg
     7441 ggaatgcgca ccagcttaag catctcgacg gcgcgcgcgt tggcctcctc ggtgccggca
     7501 ccctggtgaa tccgcaccgc ctcggcgatc tggtcgccga tggtcagcac cgggttgagg
     7561 ctggtcatcg gctcctggaa gatcatggcg acccggttgc cgcgaatgtc gcgcatccgg
     7621 gcctcgtcga gcgccaggag atcggtgccc tcgaagcgaa cactgccggc gacggtgcgg
     7681 gccagccggt cgggcaggag gcgcatgatc gagagggcgg tcacgctctt gccgcagccc
     7741 gattcgccga cgatgccgac tgtctcgccg gccatgacgt cgaacgacac gccgccgacc
     7801 gcccgggtga cgccgtcggc ggtgaagaaa tgcgtctcga ggtcccggac gctgagcagc
     7861 ggttgggtca cggcggccaa ctcacatccg ccgggcgagg cgcgggtcga gccggtcgcg
     7921 cagcccgtcg ccgagcaggt tgacggccag cacgacggtg gcgagggcga tgcctgggca
     7981 gaagatggtc cagggtgcca cgctgagata gagccggctc tgggcgatca tgttgcccca
     8041 ggtcggcacg tcaggcggcg tgccggcgcc gaggaaggat agtgccgcct ccacgaggat
     8101 ggccgaggcg cagatatagg tcgactgcac gatcaacggg gcgatggtgt tcggcaggat
     8161 gtggcggcgg atgatccgca gcgtgcgggt accgctggcg acggctgcct cgacataggg
     8221 cgcctcgcgg acgctgagca ccacggcccg caccagccgg acaacgcgcg gcacttccgg
     8281 aatggtgatg gcgacgatga cgatgaagac gctggccttg gtcagggcca ccagcgcaat
     8341 cgccagcagg atcgccggga tcgccatgag cccgtccatc agccgcatca cgaccgcgtc
     8401 caccttgcgg aagtagccgg cgagcaggcc gatcgcgagg ccgacgacga tcgagaagat
     8461 ggcgaccgag agccccacca ggagcgagat ccgggccccg tagacggtgc gcgcgaagac
     8521 gtcgcgaccg agatggtcgg tgccgaacca gaattccgcc gagggcggct tcagccgctg
     8581 cgccggggtc atggagatcg gatcgccggc gaagtagggc gctgccacgg ctgtgaccaa
     8641 gagcaggatg agaatgaggc cgcccagcgc catggtcggg ttgcgtcgca ggaaccgtgc
     8701 gaaggatggc cgggcgcgcg gtggcggtgc ggtgacggaa gcggccgccg gagcaacggg
     8761 ggcgctcaat agcggatcct cggatcgaga aagctgtagg agaggtcgac cagcaggttg
     8821 accagcacat agacgcccga gaagagcagg atgacgcctt gaatgatcgg gtagtcgcgc
     8881 ttggcgatgg catcgaccac cagccggcca acgcccggga tgttgaagac ggtctcggtg
     8941 atcaccaccc cgccgatcag cagcgccaca ccgatgccga tgaccgtgac gatcggcacg
     9001 ccggcgttct tgagcgcgtg cttgagcagc atcggccggg tggcgacccc cttggcccgg
     9061 gcggtccgga tatagtcctc cgacagcacc tcgagcatgg aggtcctggt gatccgggcg
     9121 atcagcgcca cataggcgag cccgagggcg atggtcggca ggatcaatcg ctggatccag
     9181 gggccgatgc cgtcggcgat gggcgcgtag ccctgcaccg gcagccagcg gagattgatc
     9241 gagaagagat agatgagcag atagccgacg acgaagaccg gcaccgagaa gccgagcacg
     9301 gagaagccca tgagtgcccg gtcgatcagc gtgccggccc gccacgccgc gagcacgccg
     9361 aacgagacgg cgatcaccac ggcgaacacg atggtggtga gcgacagcga gatggtcggc
     9421 tcgagccgtt cgccgatcag cttggcgacc ggctcgttgg agaagatcga gatgccgagg
     9481 tcgccctgca gcaccccgcc ggaccagcgg atgaactgca ccggcagggg gtcgtcgagc
     9541 cccagccgct cgcgcatctg ggcgatctgc tccggggtcg cgttgtcgcc ggcgacgatc
     9601 gccgccgggt caccgggcga gagatgcagg agcagaaaga cgaagacacc gacgatgccc
     9661 atgacgagca ccgtcgagcc gagccggcgg atgatatagc cgagcatcgc tcagccggag
     9721 cggcgccgcg agcggcaccc ctccttcgag ccgggtcgct ggatggcgat caggatgcct
     9781 tccgctcgat gttccagaac ggaatgatct ccgggatctt caggatgccc tcgaggttcg
     9841 agcgataggc caccggcgac acccactggc cgagccagac atgcggcacg aagttccagg
     9901 cgttctcctg gatctgccgg gcgatctcct gctgcgcctc gagcccctgg gcggccgccc
     9961 actcgtcgcg cagctcttcg tgcttctcgt ccgtgggcca gccgaaccag cccttggtgc
    10021 cgttggcggc atgggcggcg agtgcgatcg ggttgttggc cgaagcggcg ccggcccagg
    10081 tgatgaagat gttccagccg ccatcctccg gcggatcctg caccgcgcgc cgggtcacca
    10141 cgccgcccca gtcggaagtg gcgagctcgg cattcacgcc gatgtcgcgc agccgctggg
    10201 cgacgatctg cgccgcgttg ttcatgaagt cgatgttggt cgcgtgcagc acgatgaccg
    10261 gccggccatc gtagccggcc tcctcgaaga gctgcttcgc ccgctcgggg tcgggtgctg
    10321 ccgcaaacca ctccgtgttg gcgtcgttct ccatcggcgt gccgcaggcg aaattggaag
    10381 ggcacttcct gtagtagcgc gggtcgccga aggtcgcctg catgaactct tcctggtcga
    10441 tcaggtagag catcgcctgg cgcgccttga cgttgtcgaa cggcgggtag aggaaattga
    10501 gccgcatcag gccgtaattg ccggtgttgt tcagcacgtc gatgacaatg ttcggatcgg
    10561 cctcgaggac gctcaagaga tcgatcggcg ggagctccat gaaatcgatc tcgccggcct
    10621 gcagcgctgc catggcggtc tgcgagtcgt cgatgttctc gaagatcacg cgatccacct
    10681 tgacgacctt gccgccggcc atgccggaag ccggctcgga acgcggcacg tagttcgggt
    10741 tcttgtcgta gacgtactgc gcaccctgtc gggtctcctc gcgattgaag atgaagggcc
    10801 cggagccgat gtactcggtc acctgctcca tcgggtcggt catcgcctcc tgctcgcgca
    10861 tgatgtagag cagcggcgtc gaggtcttgg ccatgatgtc gatgacgagg ccatagggct
    10921 cgttcagctc gatggcgaag gtcttgtcgt ccctggcgac gatgtccttg acgcgcagca
    10981 tcatgtgctg gccggcacca tcacgcaccg cccagcggcg gatcgaggcg accacgtcgg
    11041 ccgacgtgac cggcgtgccg tcggagaacg ccagcccgtc gcgcaacacg aactcgtagg
    11101 tgagctggtc gtccgagacc gtccacgtgt cgaccatctg cggctggggc ttcatgtcct
    11161 cgtccagcgc gaagagcgtg tcgtagatca tggccccgtg gtaggcggtg atgttggccg
    11221 tggtccagat cgggtcgaag gcgcgaaggt caccctgcat gaccgccttg atggtgccgc
    11281 cggactcctg cgccatggcc cgctgccacg gcagccccgt caatgccgtg gacgcagccg
    11341 ccgtcgccat gctgccgtga acgaaagtcc tgcgtgtggt tcgcatcggt gccgttccct
    11401 tcctggtgct tgtggctggc cgtacgctgc cgcatcacgg cctgttgcat tcatgatttc
    11461 cgaattttcg ctccggcgaa agcccaaaat cccgattcgc cgcttcagac cagaaaccgc
    11521 cccggcggca gctcgtcgtg cggccagtgc gggcggctga tcttgcgata gggataaagt
    11581 cgggaatcga gcggcgatgg cccgaggcca tcgcagtaga tgacctcgct ggcgatcggc
    11641 gcgtaggccg catggaagtg gtgcgtcgac ttcacgccga tatagcgctt gctcgcgaga
    11701 tcgatcccca catcgctgaa gatctcggtg ccgagcgcct gggtccggtt gctgatcagc
    11761 gtcacgtcga cgctgccgcc ggccgtcgcg atgcggatgc cggcggcatc gccgaacgag
    11821 acctttgccg tgccgaagga ctgccagccg tcccttttca ggccgatgag cgtcacctcg
    11881 gcgtcgaccg gtgccccgga ggcggcagat gccttgccgc cgaagcgtag cgggaaggtc
    11941 gagccgatct ccttcgtgtg gcagatggcg acggcgaacg gatcccagaa cgggccgatt
    12001 gccgcatcgc cgcagccgcg ctccagcagc ctgcggagga cgttggtgtt gtcggacgcc
    12061 gccccgccgc cggcattgtc ggagggatcg gccacgacga ccggccccgc ttccgccgca
    12121 taggcgaggt cgatcgcctc gtcgatcgag caatgcgggg gtcgccaggt gccacgccgg
    12181 gcccgcacct cgtcgccgag ctcgagcgcc agcgcatcgc ccttggcctt ggcgtcgtcg
    12241 gtgacgacca gcacccgcac cccctggtcg gccacgtcgc cctgcgtgaa gccatgtgcc
    12301 agcgaaaccg agagcatctc gggccgcgcc tcgtgcacct tcatcttggc cacgaacgag
    12361 cgggccggct cgcgggtcgt cgggaagaga tcatccatcc ggcagtcgta gagcgacatg
    12421 acggggtgga tctcgccccg gatggtgcgg gtcacgaggc tcaccagctc ctccgcccgc
    12481 tcgacgaaat cggtgtgcgg gtactccttg aagaggatga tgacgttggc gttcctgacc
    12541 cgcttctcgg tcaggtggca gtgcgggtcg aacgccacgc cgataaccgc cgccggcccc
    12601 acgagctcgc ggacccgctc gatcagctcg ccctcgcagt cgtcgcaacc ctgcgccagc
    12661 atcgccccgt gcaggccgag cagcacgccg tcgaccggca aggccgcctc gagctgggcc
    12721 aggatctcgt cgcgcatcga ttcgtaatcc gcctggacca ccggggccga cggctcggcc
    12781 cagaagcagg agccctcgat cagctcgaaa ccctcttgcg ccgcgcggtt gcgcgccacc
    12841 cacaaggagg cggtggaatg ggtcggccgg tcggggtgct cgcccggccg gaaatagacg
    12901 cactcctcga agctccgccg gtcggtggga atgggcgaga aggagttcga ttcggtggcg
    12961 agggaggctg tgaagagtcg catgagcatg gttccggcgc tacgttgggt ccaaactcgc
    13021 gcggctcggt cgccggcgca agtcgcgaac ggaaggaacg ggcatggaag tcgagtggcg
    13081 gcacctgacg gcagcagcgc tgaacgaacg cgccggggcc ggcgccatcg tgctcctgcc
    13141 ggtcgcttcc accgagcagc acggccccca catggcgacc ggcaccgacg cgctgctggt
    13201 ggccgaggtc tgccgccggg tggcgctgcg catcgatggt gtcgtggcac cgacgctctg
    13261 ggtcgggctc gccgagcacc acatgagctt cggcggcagc ttcacgcttc ggctctcgac
    13321 ctaccaggcg ctgcttgccg acctgctcgt ctcgatccgc cgcgccggct tcggccgggt
    13381 ggtgatcgtc aacggacatg gcggcaacat gaccgccctc aatgccatga gcgccgagct
    13441 cagcctgggc agcgggctcg agctcgccgt caccacctat ttcctgctcg ccgaggcggc
    13501 gatggcacca catctcgagg accagccggg cgtgctccat gccggcgagg ccgagacctc
    13561 gatgatgctg gcgctgcgtc cggagtccgt cgacaccgcc cgccttgccg aggccacggg
    13621 gccgatgccg gagcggggct cggccctggc gtcacccttg catcgctggc gctcgttcgc
    13681 cgagctctcg accaccgggg tcatcggcga tgcccgccgc gccacggccc tgaagggtga
    13741 gcacctgctc gaagcggctg ccacggctct cgccgagcag ctcgccgccg gtgagccctg
    13801 ggctcaagaa caatagtccc acccgtccca cgcaactctg agagtgccac acatgacttc
    13861 cccgctcgcc gccgccatcg cccgccagta cggcacgccg gccgtggtca tcgatctcga
    13921 catcgtcgag cgcaatatcg accggctgca gcaggcctgc gacgtggccg gtctgcggaa
    13981 ccggccgcac atcaagaccc acaagtcgcc ggttctagcc gccctgcagc gcgaccgcgg
    14041 cgccaggggc atcacctgcc agaagctcgg cgaggccgag gtgatggcgg cagccgggct
    14101 cgacgacatt ctgatcagct acaacctgat cggcgaggag aagatgcagc ggctgggtcg
    14161 gctgctgcgc accggcgtca cggtgcgccc gtgctgcgac aaccccgtcg ccgccgacgg
    14221 ctatgtcgac gccgcgcggc tggccgggcg cgagctcgag gtgctggtgg aatgcgacac
    14281 cgggcgcgag cgcgccgggg tcgagacgcc ggccgaggcg atcgccctcg cccggcggat
    14341 tgccggccat gacgggctgc gcttcggcgg cctgctgctc tacgcccccg aggaccggca
    14401 ggccgaaagc caaaggttcc tggacgaggt gaaggcgggc ctgcgcgaga acggcctcga
    14461 ggcgggcatc gtctcgaccg gcggcacgcc caatctccgc aatctcggcc gcatcgaggg
    14521 tgccaccgag caccgctccg gcacctcgat cttcaacgac cggatgatga tggcctgcgg
    14581 acatgcgacg ctcgaggact gcgcgctcgc cgtctacgcc accgtcgtca gccgcgccgg
    14641 gcccgatcgc ggcatcctcg attccggctc caagacgctc accagcgaca ccggcggcgg
    14701 gctcgacggc cacgggctga tccgcgaata cccaaaggcc cggatcaagg gcttcgccga
    14761 ggagcacggc atgctcgacc tctccgcctg cgacccgaag cccggcatcg gcgagaccgt
    14821 cagcatcatc cccaaccacg tctgcgtcgt cgtcaacatg gtggacgagc tggtcacggt
    14881 gcgcggcggc gagatcaccg gcacgctccc ggtcgccgcc cgcggcaagc tgcggtaggg
    14941 cacctgcgca gggcaattgg ttaacaccga cgaaaccaat gagtacgaca gccgagcaag
    15001 aaccggggta cggcaagcac tcgtggggta ggcaatgtgt cagctccacc cttcggagcg
    15061 gccacgaccc gagaggttgc gatgtcgtcg atgccgaccg acgtggtagg aaagctgttt
    15121 gcgaagctag ctgacccggc gaaccacagc gctgcgatca tcgaagcggc cctgaacctc
    15181 gcccatgccg atgggctttc gaaggctgtc gatttagacc catggctcga gcgtttcgtc
    15241 gaggcgagca ttcgtgaact cgagggacgc tacatggggc ttttttcatt gttctggacg
    15301 gacgatgcga ttgggcgcga ggaattcgca caatatgtca cctcgtgctt cgaaccccca
    15361 tcgtcccgcg agctgacatg gccttggccc attaccgaac ccggcccaag caaaggaggt
    15421 tcacccggca tttttgagca cgagcacagc ggcctgtggt tgtgcggcta tcgaaccgga
    15481 aagacgaagg gactgccgga caatgaacgg aagctcttcc tcgcctattt cttccgccag
    15541 ccattgccac cgatcgtcgc ccaatatcac gatgatgact atggagcacc cggcaccgag
    15601 aagcgtctgc gcaaaatggc aaacctgatg gccgcatgct gcagaaattt caaatccaga
    15661 gatcgccgga aattgcgcgt cgctattgcc cactacgaag aagatcttga gtttttgcgc
    15721 agcaattttt accgtcccgg catgtttcca tggccaccga tcgaaccgtg agcctagcgt
    15781 ccgactgtcg agttcagccc agatagaact tctggatttc cccgccgatg acgaccgtac
    15841 ggtcgatcgt gtcggtcagg aactcgtgca ggccgcgctg gaagatgtcg tcgatgcggc
    15901 tgtagcggag ccgggcatgc atctcgccgg cgagtcggtg cgcctcgccg gcgcggccac
    15961 catggcgttg ggccagcgtg tcgaggtttt ccagcacctg ctcgtagcag gcgcggagcg
    16021 agcgcggcat ctcgggcctg aggatcagca tctcgaccac ctgccagggc acgacgcgac
    16081 ccttgtagag cgcgtgatag ctgcgccggg ccgagacgga cctgaggatc gccgcccact
    16141 ggtagtagtc gaggctgccg ccgacctgct cgaagctcgg caggaggacg tggtacttga
    16201 cgtcgaggat tcgggccgtg ttgtcggccc gctcgaggaa ggtgccgagc cggaggaagt
    16261 tgaagttctc ggtcctgagc agcgtgttgg tgtaggcgcc gttgaagagc agcgcctggc
    16321 gcttcgccca gtcgacgaag gtgctgaaat tgtcggagga gaaggtctcc tcgtcgaact
    16381 cgcgggcttc gagataggcg ccgttgatcg cctcccagac gtcgagcgtc agggcggcgc
    16441 gcaccgcccg ggcgttgttc cgcgcctgga cgaggcagct catgatgctc gacgggttgc
    16501 gccggtcgcg gatgagaaaa tggaggacag cggcctcgtc ggagcgttcg tgcagctcga
    16561 agaacggcgt ctcgcagccg gcagcgatga gggcggaatg ccactcgttg aggctgttgg
    16621 ggctcgccag cacggacatg tgccgggcca cgtcgagcag ccgggcgaca tagtccatgc
    16681 gctcgaccga gcgtgcgagc cagaagaggt tgctggcggt gcggctcaac atcgcctcag
    16741 ccctccccca ccgtctgcct cgtgacctgg ctctgcgact ggctctggct cgcggcctgc
    16801 gccgtcggcg ccagcaccca ggtgtccttg gtgccgccgc cctgggacga gttgaccacc
    16861 agcgagccct ccttcagggc gacgcgggtg agcccgcccg gggcgatccg cacggcgtcg
    16921 ccgccgagca gcacgaaggg tcgaaggtcg acgtggcggg gggcgacgcc gttggcgacg
    16981 aaggtcgggc aggtcgagag ggcgagggtc ggctgcgcga tgtagtcgtg cggcttcgcc
    17041 tggagcttgg cgcggaactc ctggatctcg gccttgctcg catgcggtcc gaccagcatg
    17101 ccgtagccgc ccgagccgtg caccgccttg atgaccagct cggccatgtg gtcgagcacg
    17161 taggccagat cgtccgcctc cgcgcagcgc caggtcggca cgttgttcag gatcggcgtc
    17221 tcgcccagat agaagcgaac catgtccggc acgtaggtgt agatcgcctt gtcgtcgcag
    17281 atgcccgagc ccaccgcgtt ggcgagcgtg acattgccct tgcaataggc gtcgaagagg
    17341 ccgggcacgc cgagggcgga atccgggttg aaggccaggg gatcgaggaa ggcgtcgtcg
    17401 atccgccggt agatgacgtc gacccgcttc gggccctcgg tcgtgcgcat catgacgacg
    17461 ccgttctgga cgacgagatc ctgcccctcg accagctcga cgcccatctc gtcggcgagg
    17521 aacgagtgct cataataggc gctgttgtag aagccggggg tcaggagcgc gacgaccggg
    17581 tcgcccgggc agttcggcgg cgccacgctc tgcagggtgg cgagcagctc ctcggggtag
    17641 tgcgagacgg gctggatcgc ctgccgggcg aagaggtcgg ggaagaggcg catcatcacc
    17701 tcgcggttct ccagcatgta ggagaccccg gatggcgtgc ggcagttgtc ctccagcacg
    17761 tagaactcgt ccggcccggt gcgcaccatg tcgatgccgg cgatgtgggt gtagacgccg
    17821 gccggcacct cgacgccgac catcttccgc tcgaagcagg gattgccctc gaccagccgc
    17881 tcgggcacgg tcccggcacg gatgatctcg cggccatgat acatgtcctg gacgaagcgg
    17941 ttgagcgcct tgacccgctg ctcgagaccg ctggccagga gcgcccactc gccggcgtcg
    18001 agcacgcgcg ggatgatgtc gaaggggatc agccgctccg ggtcaccgcc ttcggtatag
    18061 accgcgaagg tgatgccgat cttgcgaaag agcgcctcgg cctcccgctc cttgcgggcc
    18121 agaaagtcgg gcggggtccg actaagccaa tcctcgacca ccgcgtaggc cggccggcag
    18181 ctggcatcga gcccgtgcat ctcgtcgaac atcgccgtcc cctcgtcgcg gcaccgctct
    18241 ctggcggggc gggcctgcgc cgaacatggc aagcccgtca ccgggctgtc aatcttgccc
    18301 aacttctggg caattccggc gcaattggga ccggggcacg ggcctgcccg tcagcgcacc
    18361 agccgatagc cgccgggctc ggtcaacagt agcgtgttct cgccggcgat cggctcgatc
    18421 ttctgccgca ggcgatagac gtgcgtctcc agcgtgtgcg tcgtcaccgc cg
//
LOCUS       DAOTFI010000085        18256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3671_length_18256_cov_6.879018, whole genome shotgun sequence.
ACCESSION   DAOTFI010000085 DAOTFI010000000
VERSION     DAOTFI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18256
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3671_length_18256_cov_6.879018"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..926
                     /locus_tag="P5558_10420"
                     /pseudo
     CDS             431..926
                     /locus_tag="P5558_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553267.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1302..2006
                     /locus_tag="P5558_10425"
     CDS             1302..2006
                     /locus_tag="P5558_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY24766.1"
                     /translation="MDVKGKKALVFGGTSGIGLATAKRLAALGAEVVAISRDPSKAGD
                     VPKGITLEQCDVRDREALQALLAAQAPYDILISAATGGGRAAGPFLEMDMDGYRASFD
                     KLWGYAHVIRYGTQHLAKDGAIVVVSGSPARKSLPGQVALSSVGGAVEALVRAVAKEI
                     APRRINVVSPGFIDTPMVPKDTPNRDEVYRQRTANNLIPRAGTADEVAQAILFVVQND
                     FVTGTTVDVDGGWLLS"
     gene            complement(2019..5447)
                     /locus_tag="P5558_10430"
     CDS             complement(2019..5447)
                     /locus_tag="P5558_10430"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HRY24767.1"
                     /translation="MAMFNFSHALSTTIAQATCFVCGRIAGGRNGMRLISYTLIVILL
                     WLPPSSTYGWAERRVLPGIAEFVVPWRGTLILGTWKGIWRLTTPDAAPELVAEGMAQS
                     FELADDALFLETEIGVWYLAAPDTTPVRLAEDDDLFVKKFGGALFVGTDGGIWRIVTP
                     KAAPERLAEGWAKLVEEHSGALFLGTSDGIWSLSTPDAAPKKLADSSARFVEERGGAL
                     FLGTSEGIWLVATPDAVPRRLAEGWAQFVDESGGVFFLGTDEGVWRLETPDAASELLA
                     EGRAVFIEESGGAFFLGTDEGVWRLATPDAASERLAKGSARFVEERGDTLFLGTSQGI
                     WRLATPNAVSEKLVEGVARFAEARDGIFLLGTDHGVSRIRAPDGTLEQLLEGGDFNSL
                     ERDGAFFLWSNDGIWRLATPNAAIEKLAEGSFSFIKERQGALFWGASEGIWRVATSDA
                     APRKLAEGQAWFVEERGGALFLGTSEGIWRVATPDAASRKLAEGKAKFVEESGGAFFL
                     GTNEGVWRLATPDAASELLAGGSVEFIEDYDGALFLGTSDGISRVYDVTARVTALSTP
                     AKSAWWERSLSWLPPNTLVGPLAVPTVSYQGADAERIVVPASHFEVILAADSVTLSER
                     RERNDFQQSDKIQSVPIGFPSTLHVAIRDSWGNEYEYTLEQFVLPTAGAFGALLLIVP
                     PVLLLLALLAAPYLRFCHSLIMNPFLRGIGSFGVVPLVLTLVPLARRHLLRRYRRALL
                     ASDDIKGWAERFVPPEERFTAAAFAARLGQRSRLLIVGVSGVGKTAFLWHLTACAAEG
                     DVRPGGAAPLPIYLPLGRYAADIGVEAMIRGQLENLGELHDEQFVDWLIKHGGFLLLF
                     DGLNELGDARRDEIVTFVEQHSQHNWVVATSQDAYARQFGHWGAVETMPKLTRSGIEA
                     LLRHHLGEARAASLVATLDEQQTALLALPQNLELALELAPELADEAAGLRLPDSEEGL
                     FRAVFEQQFAAWREAGTGDFIDLLTADAYAMLRDRAPATLPPEIASPLIERKLMRQLG
                     DAVYFRHDTLRGYAASAWLAGHWRTAIEQDGAQIDANWDAMLRFTTARLLLQKNGAAE
                     VEALLFALLERAPDRSAVLFAWLEQEHADAVASYRDKFDRAFGEVTRTRLTRAA"
     gene            complement(5586..6566)
                     /locus_tag="P5558_10435"
     CDS             complement(5586..6566)
                     /locus_tag="P5558_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24768.1"
                     /translation="MTALGMTAHVQSAFSGRESRPILIACACIVVILIVGTIYTLWAH
                     GTASFLSPRYLMQQLQVGAFLGLVAAGMMLVILLGHIDLSVPWTIAAAAMMATAVGGP
                     LALPVGLGLGVAVGLVNGLGVAYLRVPSMIFTLGVNAVLRGLMVMHTGGFAPQTHSTP
                     LMEFLAVGHILGIPMALFVWILVSILIVVMLRRTPLGRYIYAVGNREAAAYLSGVNTR
                     LVLISAFVLCSTLAALAGVLLAGYSTKAYQGMGDAYLLPSIAAVVIGGTNILGGQGRY
                     TGTVVGTILIVLLTSVLSIMQMPEAGRQIIYGTVIIAMLLAYGRRDRVTS"
     gene            complement(6563..7603)
                     /locus_tag="P5558_10440"
     CDS             complement(6563..7603)
                     /locus_tag="P5558_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24769.1"
                     /translation="MSDLRIRLVQSLGLILAIGLFLLFYLIYNFNHPRGFSSQILIQN
                     ANESFVLVMVGMAQTVPALVGGLDLSVGAVMTMINTLASYLLVGWPLQILGGIVICLA
                     TAALAGFVNGCLVVYGRIQPIIVTLATGAVYMGIALFLRPTPGGKVDGDFSWAMTNDL
                     FELAATYGFAGDGDAAWFRPFAWLPLPLILMVLVVLLVWLPFKNSVTGRGVYAVGSAE
                     GAAYMSGVRVDRCKIAAFTMAGLFAGFAGLYLTLQTSSGNADVPQAGAYTLNSIAAVV
                     IGGTSLMGGSGGAVGSLFGALVIRSISFNFRIFDVDPLLQPLIEGIVLLGAVSIGAAR
                     IFRVRNKLELFG"
     gene            complement(7600..9144)
                     /locus_tag="P5558_10445"
     CDS             complement(7600..9144)
                     /locus_tag="P5558_10445"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY24770.1"
                     /translation="MGGSFLELAGISKRYGGVRALEDVSFACEQSSIHAVLGENGAGK
                     STLIKIVAGVVRPDTGTMTLEGQPVRFDGPIAANKAGIVCIFQELSLMPDLSVADNIC
                     IADPPRRFGLIDQRAQRRRAEELLARVGCADVNPLQRVRNLPLSRRQMVEIAKALGQN
                     PKLLILDEATSALTGADVEKVYRILEQLRRDGLAILYISHRMHEIEQLADTWSVFRNG
                     QHIETFARGARSADEVVQLMIGREITQVFPPKPPKASDSRPMLEVSGLSWVDRLAHIS
                     FAVHAGEIVGLGGLDGQGQRELLLALFGVLRGVEGSIRVAGQPVAVTGPSGAKAKGVD
                     MALIPEDRKTEGLMLPMSIGDNLGMAALGSLTRGIFVDESRERAAIEAMVEKLKIKIG
                     AVDDPVATLSGGNQQKVVIGKWLMTEPRIILLNDPTRGIDVGTKQELYQLLRDLAAAG
                     AAILFYTTDYDELIGCCDRVLILYDGAIVTTLEGDDLTETRIVAASLNLAAGEPGATD
                     RQAGGH"
     gene            complement(9201..10334)
                     /locus_tag="P5558_10450"
     CDS             complement(9201..10334)
                     /locus_tag="P5558_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY24771.1"
                     /translation="MMRRLLASAAILAGLGLVGPLAQAAPEVVDGPGYLPECFAPWSD
                     EITYFQWEAREGPFKIALVNGFVGNTWRIQMIQTAKAFVEQEGIKEHISDFRVISTGT
                     DAAAQLGAIEDFINQGFDAIITIAVSPEGFDRVIRLADRNNVVLVPFDNILDTDAVMM
                     VNEDQFAMGVMSAEFLIEELGKTEGKVLEVRGLPGNSVDRDRHLGFRSVMEAEGKNFE
                     IVEIVGNWDDGTSQKATADALAVHDRFDAVFTQGGSTGTVRAFIDAGHPMVPMAGEAE
                     NGFRKQIAALSDEGLKGLSYGQSPGLVAISIKAAIAALQGEKLPQLISVPIPVADYTN
                     LEDGVNYWSDLSDNFFTPNEFPPCDVNITAVEIMGKTADDAAQ"
     gene            10510..12540
                     /locus_tag="P5558_10455"
     CDS             10510..12540
                     /locus_tag="P5558_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRY24772.1"
                     /translation="MAIMLGADIGGTFTDFVAHDAATGRTEVWKVPSVPSDPVQAILD
                     GLADYTGRTALGHLRIGTTVATNAVLERQGAVVAYVTTRGFRDVPFIQRGNRKHHYDM
                     SWVKPKPLCQRRHCFELDERLDAKGRVIRPLDESEVRKVARAIAAMPEIEAVAVCLLF
                     SYLNPDHERRVKAILEEVLPDHAVSVSFEVLPKWKEYERASTTLADAYLKPVVGRRLN
                     AMRARLDDAGITAPAVLIRSNGGEMTLDAAAATPVQLLVSGPTGGVIAGRHVAELAGI
                     DHLVTLDMGGTSTDVSTVLGRRETFTTAFEIEWGVPIQIPIIDIRTIGAGGGSIAWID
                     KGGMLRVGPQSAGARPGPAAYGQGGSEATVTDANVVLGRIDPANFLGGQMQLDAAAAR
                     AAVTRVGERVGLDPEAAALAILRIADNNMVGALRSVLIERGLDPRDFALLAFGGAGPL
                     HAASLIREMGIARAVVPNHPGQFSAYGFIQTDARVDRQRTIQLTSHNFPAARAREMLA
                     ELTDQALGELQAQDYTDGITVQQALEMRYLGQNYELELAIGEDALADDRIQALWQSFH
                     AAHEARFGFAIPGEPIEIVNLSVTAISTTAKPDPAKLDVDMPEGRPVAERPVHFAGGT
                     VATRVYSRPTLAPGQRIQGPALIEEAASVTVVEPGQHLVVDAYGHLVISVGA"
     gene            12593..12844
                     /locus_tag="P5558_10460"
     CDS             12593..12844
                     /locus_tag="P5558_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019334760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY24773.1"
                     /translation="MTTLTVTAKGQVTLRKDVLRHLGISPGQKIALHLLPDGRAELRA
                     ARPTGTINDFIGLLAGKTDKVISIDEMNEAIAAGWAGEE"
     gene            12841..13239
                     /locus_tag="P5558_10465"
     CDS             12841..13239
                     /locus_tag="P5558_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY24774.1"
                     /translation="MRITVDTNVLVRAVLRDDEGQTRIAARILNEASLIAVSLPCLCE
                     LVWVLRRGARRPKEEISAAIRALIDADNVVVDRPAVEAGLALHDAGGDFADGVIAHQG
                     NWLGGETFVTFDKRAVAKLAAQGVAARHLA"
     gene            13276..15189
                     /locus_tag="P5558_10470"
     CDS             13276..15189
                     /locus_tag="P5558_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY24775.1"
                     /translation="MKFSANGYYVERYNKCANCGMLVYGEGLMAGHRGKSRRFCSDWC
                     IEWAGLRDGADGLVRLPIPRPAPVERTAAPDMVMMNILDSTMVSICREMGILLMKTSY
                     STIFNEGLDFTCALADAKGDMIACAEFCPTMIGGMPLLIKTCFQEIPHEDIEPGDIIL
                     HNDPYRGGLHCPEHTFFKPFFDGGELLGFAVCIGHIAEIGGMVPGAFCGEATEIFHEG
                     MRVPPVKIKKGGKDVDEVWKLLLANTRTPRQNYGDLRAMIGSVDLGVDRIGQIVKKYG
                     REVFKNTCEDLMDYAERRMRAELGAFPDGKYSFHDVIENDGIEAKPYTVAIDLHINGD
                     ELVADYDRSSKQARGPINATLGVATGAVYNGMLHVTDPSIPKNSGCFRPIRVVSPPGK
                     VTNVDFPGPLVAGNTETHPRLANITIGAMSKCAPERAMASESCTGTNFVFGGEHPEYD
                     EYFACYDIMSGGWGGRHGHDGNDCVIAINGNCRFNPTEVFETRFPLRVEDCAMDTDSG
                     GAGEFRGGLGYTRTLLITEVDITGSQCSDRHEVKPFALFGGEPGGNGATLIKKAGSDQ
                     WLTVKELYGKVSSSKYANVRFQPGDRIRLTAPGGGGYGEPAKRDPAAVALDVAEGYVS
                     AAAAERLYGKGGA"
     gene            15189..16115
                     /locus_tag="P5558_10475"
     CDS             15189..16115
                     /locus_tag="P5558_10475"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRY24776.1"
                     /translation="MRIAMLGAGAMGGMFGARFARAGAEVVLYDRDAAHVAAIAADGL
                     TVELPDEEPITLRLPAFAEALAIGTVDLAVVLVDCAATRDAALVAKDVIGTGGFALTL
                     QNGIGNVEALVDVLGAARVVAGSTMNSAARLGLGRVAHTHLGKSVLGELDGRASERLE
                     RLAALFDRGGLPVATTDNIMGHVWQKFVLNAALNPVCAITRLRPGEVAESASACALVR
                     DILREVLAVVAAEGIDLPDPDPTEGILGHAVGRMNKPSMLQHVEQGRRTEIAALNGAL
                     LPIAARHGIPCPTNRTIVQLVEAIQGRAARTA"
     gene            16234..>18256
                     /locus_tag="P5558_10480"
     CDS             16234..>18256
                     /locus_tag="P5558_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HRY24777.1"
                     /translation="MSFPIIGIGSSAGGLEPLQALIRAVPEASGLAFVIVAHLDPTQK
                     SHLPELLARCTAMPVMQVEETTEVRPDHVYVIGPDQDLSIEGGALHTKKSELPRGHRH
                     PVDVFFRSLAEDQGRRAIGIVLSGTGTNGSLGLRAIKAKGGIAIAQLPETASFQGMPR
                     SAIGTGVVDLVLAPEQMPEALLDLARHPYVRAPKTTEERAADDSLKAVLSVVTARTRR
                     DFTGYRQRTLVRRIHRRMGLLKMTELRAYADRLRQDPDETRALVADMTINVTGFFRDP
                     EAWQTLAETVIAPLVRERDPGTEIRAWLPGCSTGEEAYSLGMLIVEEAERAGKSFDVK
                     IFATDIAEGVLGTARGGHYPGSIALDVGEERLSRFFVQVDDSYTVRPVVRELVTFAPQ
                     DLLRDPPFSKLDLVSCRNVLIYLEPEHQKRLLALFHFALRPDGCLFLGSSENVSGLED
                     QFQPISKKWRIFRRAGPTPSEIVDFPLGAGRGSRAGGLDTLPAGVGELRARAADVMDR
                     ALLERHAPASALVDERYRIHFMRGPIDDYLKPPVGEPTFDLLAMAREGQQMALRTAIR
                     RALDENREIVALARVRRGGERRHVRLVVTPLRRGAQAPARLLIGFFEHDPATEKVEAD
                     VPERSPGESLIQAELDNAREDLRLSVEQMESTNEELKASNEEIRSINEEL"
BASE COUNT         3131 a         5891 c         6392 g         2842 t
ORIGIN      
        1 catcgctcca tcctctcaag agttggagcc tccggcaaac ccggggcggt tcaacatgac
       61 aggttgaaca gcaaccggtc caatccggag aactggccgg ccaaaggtca gctacaccac
      121 gtcccgggac acgatcgttc cggagctttc ggattctgtc gaaatgctaa cccaacaaat
      181 tcgcaaacct gggccgcagg gctttcagac atctaagcgt gtggtccttt gggtatgttc
      241 gctgcgactg gagagttaaa tcatcgcgca ggaactagct aattcgctga cgatagcggg
      301 ttctggacga gaaaagcaat cccccgcgcg cttcacagcg taccaggctc aaccgtaggg
      361 ccgctcctga ccctgccccc tggagtgacc ccgtttcttg gttgggcccc gtcactgaag
      421 gagacggacg atgcggaaga gcaggttcag cgaggagcgg atgatcgtgg ttctgaagga
      481 tgcccaggca ggcctgccgg tggcggaggt ttgccgcggg cacggcatca gcggcgcgac
      541 gttctacgtg tggcgatcga agtatgacga gctcgagcgt cggcttggat cccaaaacgt
      601 accgctacaa cacaggccga ccgcacaatg cggcactgtg tcggcggttg cgtgggctgg
      661 cagccgcgcg acgccggttc ggcaatcgcc gcctgctgat ccggccccgc cgcgagggct
      721 tcgcggtgaa ccacaaatgc ctgttccggc tctatcggga ggagcggctg acagtacgca
      781 agcgcggcgg ccgcaaacac gcgctcggca cacgggcgcc gctaggcctg ccggtgcaac
      841 cgaacgagcg ctggagcctc gacttcgcct ccgacgtgct gaccgatggc caccgcttcc
      901 gggtcctggt cgtcatggac gactggcgct tcgactattc ggtggtgtgc agggcgcctc
      961 agttcggatc aggtcgatgc aggtcccgga cggaccgatc ctctatgggc cggtgagcac
     1021 gatgggcgcc gccgggtacc tcagaccggc aatgaagatg cggccggggc cgcccccccc
     1081 gcggagaggc tgctcatctt tgggatggct tcggtgctcg ggtgccgatg ctcgaccgtg
     1141 caacggccgg cacggcaaga gagaagggcg cgactggcgg caagctgcgg ccgccacggg
     1201 ggcttgcccc gggtgcgcgc aggtgcgaca cgggagccgg tcgctggcgg ggcatcgatt
     1261 tcgaccagtc gtattcaatt gcgcggggag tatccgggga catggacgtc aagggcaaga
     1321 aagcgctcgt gttcggtggc acgtcgggca tcgggctcgc gacggcgaag cggctggcgg
     1381 cgctgggtgc cgaggtggtg gcgatcagcc gggacccgtc gaaggccggc gacgtgccca
     1441 aagggatcac gctcgagcag tgcgacgtgc gcgaccgcga ggcgctgcag gcgctgctcg
     1501 ccgcccaggc gccctacgac atcctgatca gtgcggcgac cggcgggggg cgcgccgccg
     1561 ggccgttcct cgagatggac atggacggct acagggcgtc cttcgacaag ctctggggct
     1621 atgcgcacgt catccgctac ggcacgcagc acctggcgaa ggacggtgcg atcgtggtgg
     1681 tgagcggctc gccggcgcgc aagtccctgc cgggccaggt ggcgctgagc tcggtcggcg
     1741 gtgccgtcga ggccctggtc cgggccgtgg cgaaggagat cgcaccgcgg cgcatcaacg
     1801 tcgtctcgcc cggcttcatc gacacgccga tggtgccgaa ggacaccccg aaccgcgacg
     1861 aggtctatcg gcagcggacg gcgaacaacc tcatcccgcg cgccggcacg gccgacgagg
     1921 tggcccaggc catcctcttc gtcgtgcaga acgacttcgt caccggcacg acggtggacg
     1981 tggacggcgg ctggctcctg tcgtgagggc ggccggggct aggccgcacg tgtcagacgg
     2041 gtgcgggtaa cctcgccgaa ggcgcggtcg aacttgtcgc ggtagctggc gacggcgtcg
     2101 gcgtgctcct gctcgagcca ggcgaagagg acggctgatc ggtcgggtgc ccgctcgagg
     2161 agggcgaaga gcagggcttc gacctcggcg gcgccgttct tctggagcag caggcgggcg
     2221 gtggtgaagc gcagcatggc gtcccagttg gcgtcgatct gcgctccgtc ctgctcgatg
     2281 gcggtgcgcc agtgtccggc cagccaggcc gaggcggcat agccgcgcag cgtgtcgtgg
     2341 cggaaataga cggcgtcgcc gagctgccgc atcagcttgc gctcgatcag cgggcttgcg
     2401 atctcgggcg gcagggtcgc cggcgcgcgg tcgcgcagca tggcgtaggc atcggcggtc
     2461 aaaaggtcga tgaagtcgcc ggtgccggcc tcgcgccagg cggcgaactg ctgctcgaag
     2521 accgcccgga acaggccttc ctcggaatcg ggcaggcgta gccctgccgc ctcgtccgcc
     2581 agctccgggg ccagctccag cgccagctcc aggttctgcg gcaaggccag aagtgccgtc
     2641 tgctgctcgt cgagcgtggc gaccaggctc gcggcgcggg cctcgccgag gtggtgtcgc
     2701 aggagagctt cgatgccgga cctggtcagc ttcggcatgg tctcgacggc gccccaatgg
     2761 ccgaactggc gtgcataggc gtcctgcgag gtggcgacga cccagttgtg ctgcgaatgc
     2821 tgctcgacga aggtgacaat ctcgtcgcgc cgcgcatcac cgagctcgtt cagtccgtcg
     2881 aagagcaaga gaaagccgcc gtgcttgatc aaccagtcga cgaactgctc gtcatgcagc
     2941 tcgccgaggt tctcgagctg gccgcggatc atggcctcga ctccaatgtc tgcagcgtag
     3001 cggccgagcg gcagatagat cggcagtggc gcagcaccac ccggcctgac gtcgccctca
     3061 gccgcgcagg cggtgaggtg ccacaggaac gccgtcttgc cgacgccgga gacgccgacg
     3121 atcaggaggc ggctccgttg gcccagccgc gcggcgaatg cggctgcggt gaacctctcc
     3181 tccggcggca cgaagcgctc ggcccagcct ttgatgtcgt cgctcgccag tagtgcccgg
     3241 cggtagcggc gaagcaggtg ccggcgcgct agcggtacca gtgtcagcac cagcggcacg
     3301 acaccgaacg agccgatgcc gcgcaggaac gggttcatga tgaggctgtg gcagaaacgc
     3361 aggtagggag cggccaacag cgccaagagc agcaacactg gtggaacgat cagcagcaaa
     3421 gcaccgaatg caccggcagt gggcaacacg aactgttcga gtgtgtactc atactcattg
     3481 ccccaagaat cacgaatcgc gacatgaagg gtcgagggaa agccgattgg cacggactgg
     3541 atcttgtctg actgctgaaa gtcgttgcgc tcgcgccgct cgctcagtgt tacgctatca
     3601 gcggccagga tgacctcaaa gtggctggcc ggcacgacaa tacgctccgc atcggcccct
     3661 tgatacgaga cagtcggcac tgcgagcggc ccgaccaacg tgttgggcgg cagccaggac
     3721 aggctacgtt cccaccaagc agacttggca ggcgtagaca aggcagtcac gcgtgcggtc
     3781 acgtcataga ctctgctgat cccatcactc gttccgagga acagggcacc gtcatagtcc
     3841 tcaatgaatt caacggagcc tccggccagc agttcagagg cggcgtcggg cgtcgccaga
     3901 cgccaaacac cctcgttcgt cccgaggaag aaggcgccgc cgctctcctc gacgaactta
     3961 gccttccctt cggccaactt tctggacgcg gcgtcaggcg ttgccacgcg ccatattccc
     4021 tcagaagtcc cgagaaacag agcgccgccg cgctcttcga cgaaccaggc ctgtccttcg
     4081 gccaactttc tgggcgcggc gtcagacgtc gccacgcgcc atattccctc actggctccc
     4141 cagaagagag caccctggcg ctccttaata aaactgaacg agccttcggc cagcttctcg
     4201 atggcggcat taggtgtcgc caggcgccaa atcccatcgt tcgaccacag gaagaaagca
     4261 ccatcgcgct caagactgtt gaaatctcca ccttcaagca gctgctcaag tgtgccgtct
     4321 ggcgctctta tgcgcgatac tccgtggtcc gtgccgagga gaaaaatgcc gtcgcgcgct
     4381 tcggcgaacc gagccacccc ttcgaccaac ttctcagaga cggcgttggg cgtcgccagt
     4441 cgccatatcc cttggctggt cccgaggaac agggtgtcgc ccctttcctc aacgaaccga
     4501 gctgagcctt tggccagccg ttcagaggcg gcgtcgggcg tcgccaggcg ccaaacgccc
     4561 tcatccgtcc cgaggaagaa ggcgccgccg ctttcctcga tgaacacggc ccggccttcg
     4621 gccaacagct cagaggcggc gtcaggagtc tcgaggcgcc aaacgccctc gtccgtcccg
     4681 aggaagaaaa cgccgccgct ctcgtcgacg aactgagccc agccttcagc caaccttctg
     4741 ggcacggcgt cgggcgtcgc cacaagccat atcccttcag aggtcccgag gaacagagcg
     4801 ccacccctct cctcaacgaa ccgagctgag ctgtcggcca gcttcttggg ggcggcgtcg
     4861 ggcgtcgaca aactccagat cccgtcactc gtcccgagaa acaaagcgcc gctatgctcc
     4921 tccaccaact tagcccatcc ctcggccagc ctttcgggag cagctttggg agtgactatg
     4981 cgccatatcc caccatctgt tccgacgaag agagcccccc caaacttctt tacgaataag
     5041 tcgtcgtctt cagccaatcg taccggggtg gtgtccggag ccgccaagta ccagacaccg
     5101 atttccgtct cgaggaacaa agcgtcatct gccaactcga atgattgagc catgccttcg
     5161 gcgactagtt cgggcgctgc gtccggcgtt gttaggcgcc aaataccttt ccacgttccg
     5221 aggatgaggg tgccacgcca aggcacgacg aactcggcga tacctggaag aactcttctt
     5281 tctgcccaac catacgtcga gctcggcggc agccaaagca ggatcactat caatgtataa
     5341 gaaatcagtc gcatcccgtt gcggccacca gcgatgcggc cgcaaacgaa acatgttgct
     5401 tgggctatcg tggtagacaa ggcgtggctg aaattgaaca tagccatcac tagatgcaaa
     5461 ggcgatcgcg gaggcaagga taaaggccac agggattgat cgccctgccc caaatgcgcg
     5521 ccaatacgaa agccctgtgg ggtgcgggga gatcatctcc ccgcctgcct ggtctttctt
     5581 ccgcctcagc tcgtcacgcg atcccgcctg ccataggcca gcagcatggc gatgatgacg
     5641 gtgccgtaga tgatctggcg gccggcttcg ggcatttgca tgatggagag gacggaggtc
     5701 aggaggacga tgaggatggt gccgacgacg gtgccggtgt agcggccctg gccgcccagg
     5761 atgttggtgc cgccgatgac gacggcggcg atggacggca gcaggtaggc gtcgcccatg
     5821 ccctggtagg ccttggtgga gtagccggcg aggagcacgc cggcgagggc ggcgagggtg
     5881 ctgcagagga cgaaggcgct gatcaggacg aggcgggtgt tgacgccgga gaggtaggcg
     5941 gcggcctcgc ggttgccgac ggcgtagatg tagcggccga ggggagtcct gcgcagcatg
     6001 acgacgatga ggatggagac gaggatccag acgaagagcg ccatggggat gccgaggatg
     6061 tggccgacgg ccaggaattc catgagcggg gtggagtggg tctgcggcgc gaagccgccg
     6121 gtgtgcatga ccatgaggcc gcgcagcacg gcgttgaccc cgagggtgaa gatcatcgaa
     6181 ggcacgcgca gataggcgac gccgaggccg ttgacgaggc cgacggcgac gccgaggccg
     6241 aggccgaccg gcagcgccaa ggggccgccc acggcggtcg ccatcatggc ggcggcggcg
     6301 atggtccagg gcacggagag gtcgatgtgg ccgagcagga tgaccagcat catgccggcg
     6361 gcgacgaggc cgagaaaggc gccgacctgc agttgctgca tcaaatagcg cggcgagagg
     6421 aagctcgcgg tgccatgagc ccagagcgtg tagatggtgc cgacgatcag gatgacgacg
     6481 atgcaggcgc aggcgatgag gatgggccgg ctctcgcggc cgctgaacgc ggattgcacg
     6541 tgggcggtca tccccaaggc agtcatccaa agagctccag cttgttcctg accctgaaga
     6601 tgcgggcggc gccgatgctg accgcaccca ggagcacgat gccctcgatc agcggctgga
     6661 gcagcgggtc gacgtcgaag atgcggaagt tgaaggagat tgagcggatc accaaggcgc
     6721 cgaagagcga gcccacggca ccgccgctgc cgcccatgag cgaggtgccg ccgatcacca
     6781 cggcggcgat ggagttgagc gtgtaggcgc cggcctgcgg cacgtcggca ttgcccgacg
     6841 aggtctggag cgtgaggtag agcccggcga agccggcgaa gaggccggcc atggtgaagg
     6901 ccgcgatctt gcagcggtcg acgcgcacgc ccgacatgta ggcggcgccc tcggccgagc
     6961 ccacggcata gacgccgcgg ccggtcaccg agttcttgaa gggcagccag acgagcagga
     7021 cgacgagcac catgaggatg agcggcaggg gcagccaggc gaagggccgg aaccaggccg
     7081 cgtcgccgtc cccggcgaag ccgtaggtgg cggcgagctc gaagaggtcg ttggtcatcg
     7141 cccaggagaa gtcgccgtcg accttgccgc ccggcgtcgg ccggaggaag agggcgatgc
     7201 ccatgtagac ggcgccggtc gcgagcgtga cgatgatcgg ctggatgcgg ccgtagacga
     7261 cgaggcagcc attgacgaag ccggcgagcg cggcggtggc gaggcagatg acgatgccgc
     7321 ccaggatctg caggggccag ccgacgagga ggtagctggc cagcgtgttg atcatggtca
     7381 tgaccgcgcc gaccgagagg tcgagcccac cgaccagggc cggcacggtc tgcgccatgc
     7441 cgaccatcac caggacgaag gattcgttgg cgttctggat gaggatctgg ctcgagaagc
     7501 cgcgcgggtg gttgaagttg tagatcaggt agaagaggag gaagaggccg atggcgagga
     7561 tcaggccgag gctctggacg aggcggatgc ggagatcgct caatgcccgc ccgcctgccg
     7621 gtcggtggcg cccggctcgc cggcggcgag gttgagcgac gcggccacga ttcgggtctc
     7681 ggtgaggtcg tcgccttcga gggtggtcac gatcgccccg tcatagagga tgagcacgcg
     7741 gtcgcagcag ccgatcagct cgtcgtagtc ggtggtgtag aagaggatgg ccgccccggc
     7801 ggcggcgagg tcgcgcagga gctggtagag ctcctgcttg gtgccgacgt cgatgccgcg
     7861 ggtcgggtcg ttgagcagga tgatcctggg ctcggtcatc agccacttgc cgatcaccac
     7921 cttctgctgg ttgccgccgg agagggtagc aaccgggtcg tcgacggcgc cgatcttgat
     7981 cttgagcttc tcgaccatgg cctcgatcgc cgcccgctcg cggctctcgt cgacgaaaat
     8041 gccgcgggtc agcgagccaa gggccgccat gccgagattg tcgccgatcg acatgggcag
     8101 catcagcccc tcggtcttgc ggtcctcggg gatgagcgcc atgtcgacgc ccttcgcctt
     8161 cgccccggac gggccggtga cggcgaccgg ctgcccggcg acgcggatgc tgccctcgac
     8221 gccgcggagc acgccgaaga gggcgaggag gagctcgcgc tggccctgcc cgtcgagccc
     8281 gccgagaccg acgatctcgc cggcatggac ggcgaacgag atgtgggcga ggcggtcaac
     8341 ccaggagagg ccggagacct cgagcatcgg ccggctgtcc gacgccttcg gtggcttggg
     8401 cgggaagacc tgggtgatct cgcggccgat catgagctgc acgacctcgt cggcgctcct
     8461 ggcaccgcgg gcgaaggtct cgatgtgctg gccgttgcgg aacacggacc aagtgtcggc
     8521 gagctgctcg atctcgtgca tgcggtggct gatgtagagg atggcgaggc cgtcccggcg
     8581 aagctgctcg aggatgcggt agaccttctc gacgtcggca ccggtcaggg ccgaggtcgc
     8641 ctcgtcgagg atgagcagct tcgggttctg gcccagggcc ttggcgatct cgaccatctg
     8701 gcggcgcgac aggggcaggt tccgcacccg ctgcagcggg ttgacgtccg cgcagccgac
     8761 gcgggcgagc agctcctcgg cccggcggcg ctgggcacgc tggtcgatca ggccgaagcg
     8821 gcgcggcggg tcggcgatgc agatattgtc ggcgacgctg aggtccggca tcagcgacag
     8881 ttcctggaag atgcagacga tgccggcctt gttggcggcg atcggcccat cgaagcggac
     8941 gggctggccc tccagtgtca tggtcccggt atcggggcgg accaccccgg caacgatctt
     9001 gatgagcgtc gacttgccgg cgccgttctc gccgagcacg gcatggatcg agctctgctc
     9061 gcaggcgaag gacacgtcct ccagcgcgcg cacaccgcca tatcgcttgc tgatgccggc
     9121 aagctcgagg aagcttccgc ccattacgaa atccgcgagg cggcgatgcc cggcgccggg
     9181 ccgggcatcg cgccggtcac ctactgggcc gcgtcgtcgg cggtcttgcc catgatctcc
     9241 accgccgtga tgttgacgtc acagggcggg aactcgttgg gcgtgaagaa attgtcggaa
     9301 aggtccgacc agtagttcac gccgtcctcg aggttggtgt agtcggcgac cgggatcggc
     9361 accgagatga gctgcggcag cttctcgccc tggagggcgg cgatcgccgc cttgatcgag
     9421 atggcgacga ggccgggcga ctggccgtag gagaggccct tgagaccctc gtcgctgagg
     9481 gctgcgatct gcttcctgaa gccgttctcg gcctcgcccg ccatcggcac catcgggtgg
     9541 ccggcgtcga tgaaggcgcg cacggtgccc gtcgagccgc cctgggtgaa gacggcgtcg
     9601 aagcggtcat gcacggccag cgcgtcggcc gtggccttct gcgaggtgcc gtcgtcccag
     9661 ttgccgacga tctcgacgat ctcgaaattc ttgccctcgg cctccatgac cgagcggaag
     9721 cccagatgcc ggtcgcggtc gaccgagttg ccgggcaggc cccggacctc gaggaccttg
     9781 ccttcggtct tgcccagctc ctcgatcagg aactcggccg acatgacgcc catggcgaac
     9841 tggtcctcgt tgaccatcat cacggcgtcg gtgtcgagga tgttgtcgaa gggcaccagg
     9901 accacgttgt tgcggtcggc gagccggatc acccggtcga agccctcggg gctgaccgcg
     9961 atggtgatga tcgcgtcgaa gccctggttg atgaagtcct cgatggcgcc gagctgggcg
    10021 gcggcgtcgg tgccggtcga gatcacgcgg aagtcggaga tgtgctcctt gatgccttcc
    10081 tgctcgacga acgccttggc ggtctgaatc atctggatcc gccaggtgtt gccgacgaag
    10141 ccgttgacca gggcgatctt aaagggcccc tcgcgggctt cccactggaa gtacgtgatc
    10201 tcgtcgctcc agggcgcgaa gcactcgggc aggtagccgg ggccgtcgac gacctccggt
    10261 gctgcctggg ccaagggccc gacgagaccg agcccggcca agatggcggc actcgccaaa
    10321 agacgacgca tcattgttca gcctcctgct tgtcttcttg gacggatgcc tatcccgcga
    10381 tgcgagattt ggcaagatgc acgctcgggc ggtgccatcc ggtattgccg gcggccggca
    10441 ctgacaggta agtaaaggcc gccggcgacg ctgtcgcccc gaggccgcgc aggggagtga
    10501 acgacaaaca tggcgatcat gctcggtgcc gatatcggcg gcacgttcac ggacttcgtc
    10561 gcccacgacg ccgccaccgg tcgcaccgag gtgtggaagg tgccgtcggt gcccagcgac
    10621 ccggtgcagg cgatcctcga cgggctcgcc gactacaccg ggcggacggc gctcggccat
    10681 ctcaggatcg gcaccaccgt cgcgacgaac gccgtgctcg agcgccaggg tgcggtggtc
    10741 gcctacgtga cgaccagggg ctttcgggac gtgccgttca ttcagcgcgg caaccgcaag
    10801 caccactacg acatgagctg ggtcaagccg aagccgctct gccagcggcg gcactgcttc
    10861 gagctcgacg agcggctgga cgccaaaggg cgcgtcatcc ggccgctcga cgagtccgag
    10921 gtgcgaaagg tggcccgggc catcgccgcc atgccggaga tcgaggcggt cgcggtctgc
    10981 ctgctcttct cctacctcaa cccggaccac gagcggcggg tcaaggcgat cctcgaggag
    11041 gtgctaccgg accatgccgt ctcggtcagc ttcgaggtgc tgccgaagtg gaaggagtac
    11101 gagcgggcgt cgacgacgct ggccgacgcc tacctcaagc cggtggtcgg ccgccggctg
    11161 aacgcgatgc gcgcacggct ggacgatgcc gggattacgg caccggccgt gctgatccgc
    11221 tcgaacggcg gcgagatgac cctggatgcg gcggcggcga cgccggtcca gctcctcgtc
    11281 tccgggccga cgggtggggt gatcgccggg cggcacgtgg ccgagcttgc cggcatcgat
    11341 catctcgtga ccctcgacat gggcggcacc tcgaccgacg tctcgaccgt gctgggccgg
    11401 cgcgagacct tcaccacggc cttcgagatc gagtggggcg tgccgatcca gatcccgatc
    11461 atcgacatcc gcaccatcgg cgccggtggc ggctcgatcg cctggatcga caagggcggg
    11521 atgctccggg tcggaccgca gagcgccggt gcgaggccgg gcccggcagc ctacggccag
    11581 ggcggcagcg aggctacggt gaccgacgcg aatgtggtgc tcgggcggat cgatcccgcg
    11641 aacttcctcg gcgggcagat gcagctcgac gccgccgccg cgcgggcggc ggtgacccgg
    11701 gtcggcgagc gggtcggcct cgacccggaa gcggcggcgc tggccatcct caggatcgcc
    11761 gacaacaaca tggtgggcgc gcttcgctcg gtgctgatcg agcgcggcct cgacccccgc
    11821 gacttcgcgc tcttggcctt cggcggggcg gggcccctgc acgccgcctc gctgatccgc
    11881 gagatgggca tcgcccgcgc ggtcgtgccg aaccatccgg gccagttctc ggcctacggc
    11941 ttcatccaga ccgatgcccg ggtcgaccgg cagcggacca tccagctcac cagccacaac
    12001 ttcccggcgg cgcgggcccg ggagatgctg gcggagctga cggaccaggc gctgggcgag
    12061 ctgcaggcgc aggactatac cgacggcatc accgtgcagc aggcgctgga gatgcgctat
    12121 ctcgggcaga actacgagct cgagctggcg atcggcgagg acgccctggc cgacgaccgg
    12181 atccaggcgc tctggcagtc gttccatgcg gcgcacgagg cacgtttcgg cttcgcgatc
    12241 ccgggcgagc cgatcgagat cgtcaacctc tcggtgaccg cgatctcgac gacggccaag
    12301 cccgatccgg cgaagctcga tgtcgacatg ccggagggcc ggccggtggc cgagcggccg
    12361 gtgcattttg ccgggggcac ggtggcgacc cgggtctaca gccggccgac cctggcgccg
    12421 ggccagcgaa tccagggccc ggcgctgatc gaggaggcgg cgtcggtgac tgtcgtcgag
    12481 ccgggccagc atctcgtagt cgacgcgtac ggccatctcg tcatcagcgt cggcgcttga
    12541 atgcaaggcg gttttgcctt acttttcggg gcagccaact ggaggccgac ccatgacgac
    12601 cctcactgtc accgccaagg gacaggtcac cttgcgcaag gacgtgctca gacatctcgg
    12661 aatcagtccg gggcagaaaa tcgcgctgca cctgctgccg gatgggcggg cggagctgag
    12721 ggccgcacga ccaaccggca cgatcaacga cttcatcggc ttgctcgcag gcaagacgga
    12781 caaggtgatc tcgatcgacg agatgaacga ggccatcgcc gcgggctggg ccggcgagga
    12841 atgaggatca cggtcgatac gaatgtgctc gtccgggcag tgctgcgcga cgacgagggt
    12901 cagacccgga tcgccgcgag gatactgaac gaagcgtcgt tgatcgccgt gtccctgcct
    12961 tgcctgtgcg agctcgtgtg ggttctgcgg cgtggcgccc gccgacccaa ggaggagatt
    13021 tcggcggcga taagagcact tatcgacgcg gacaatgtcg tcgtcgaccg gcccgccgtg
    13081 gaagcgggtc tggcgctgca tgacgccggt ggcgacttcg cggacggagt catcgcgcac
    13141 caaggcaact ggctcggcgg tgagaccttc gtcactttcg acaagagggc ggttgcgaaa
    13201 ctcgccgccc agggagtagc ggcgcgacac ctggcctgag cggttccatc catccataat
    13261 tgagggcgtt cgacgatgaa attctccgcc aacggctact atgtcgagcg ctacaacaag
    13321 tgcgcgaatt gcgggatgct ggtctatggc gagggcctga tggccgggca tcgggggaag
    13381 tcgcggcgct tctgctcgga ctggtgcatc gagtgggccg gcttgcgcga tggtgccgac
    13441 ggcctcgtcc ggctgccgat cccccgccct gccccggtcg agcggacggc ggcgccggac
    13501 atggtgatga tgaacattct cgattccacc atggtctcga tctgccgcga gatgggcatt
    13561 ttgctcatga agacgtcgta ctcgacgatc ttcaacgagg ggctcgactt cacttgtgcc
    13621 ctcgccgacg ccaagggcga catgatcgcc tgcgccgagt tctgcccgac gatgatcggc
    13681 ggcatgccgc tcctgatcaa gacgtgcttc caggagatcc cgcacgagga catcgagccg
    13741 ggcgacatca tcctgcacaa cgatccctat cgcggcgggc tgcactgccc cgagcacacc
    13801 ttcttcaagc ccttcttcga cggcggcgag cttctcggct tcgccgtctg catcggccat
    13861 atcgccgaga tcggcggcat ggtgccgggc gccttctgcg gcgaggcaac ggagatcttc
    13921 cacgagggga tgcgggtgcc gccggtcaag atcaagaagg gcggcaagga tgtcgacgag
    13981 gtctggaagc tgctgctcgc caatacccgc acaccccggc agaactatgg tgaccttcgg
    14041 gcgatgatcg gctcggtcga tctcggtgtc gaccggatcg gacaaatcgt gaagaagtac
    14101 gggcgcgagg tgttcaagaa cacctgcgag gacctgatgg actatgccga gcggcggatg
    14161 cgggccgagc tcggcgcctt tcccgacggc aagtattcgt tccatgacgt gatcgagaac
    14221 gatggcatcg aagccaagcc ctacacggtc gccatcgacc tgcacatcaa tggcgacgag
    14281 ctggtggccg actacgaccg gagctcgaag caggcgcgag ggccgatcaa cgccacgctc
    14341 ggcgtggcca ccggtgccgt ctacaacggc atgctgcacg tcaccgatcc ctcgatcccg
    14401 aagaactccg gctgctttcg cccgatccgc gtggtctcgc caccgggcaa ggtgacgaat
    14461 gtcgactttc ccgggccgct cgtcgccggc aacacggaga cccacccgag gctcgccaac
    14521 atcaccatcg gcgcgatgtc caaatgcgcg cccgagcggg ccatggcgtc ggagagctgc
    14581 accggcacca atttcgtctt cggtggcgag cacccggaat atgacgagta cttcgcctgc
    14641 tacgacatca tgtcgggcgg ctggggcggc cggcacggcc acgacggcaa cgactgtgtc
    14701 atcgccatca acggcaattg ccgcttcaac ccgaccgagg tgttcgagac gcgctttccc
    14761 ttgcgggtcg aggactgcgc catggacacc gattcgggcg gggcgggcga gttccggggc
    14821 gggctcggct acacccgcac gctcctgatc accgaggtcg acatcaccgg ctcgcagtgc
    14881 agcgaccggc acgaggtcaa gcccttcgcg ctcttcggtg gcgagcccgg cggcaacggg
    14941 gctaccttga tcaagaaggc cggcagcgac caatggctga ccgtgaagga gctctacggc
    15001 aaggtgtcgt cgagcaagta cgccaatgtc cgcttccagc cgggcgaccg catccgcctc
    15061 accgccccgg gcggtggcgg ctacggcgag ccggcgaagc gcgatccggc ggccgtggcg
    15121 ctcgacgtcg ccgagggcta tgtcagcgcg gcggcggcgg agcggctcta cggcaagggc
    15181 ggcgcctgat gcggatcgcc atgctcggtg ccggggcgat gggcggcatg ttcggcgccc
    15241 ggttcgcccg ggccggggcc gaggtcgtgc tctacgaccg ggacgcggcg catgtggcgg
    15301 cgatcgcggc ggacggactc accgtcgagc tgcccgacga ggagccgatc acgctgcgcc
    15361 tgccggcctt tgccgaggcc ctggcgatcg gcaccgtcga tctcgccgtc gtcctggtcg
    15421 actgtgcggc gacgcgcgac gccgccctcg tcgccaagga cgtcatcggc acgggaggct
    15481 tcgcgctgac gctgcagaac ggcatcggca atgtcgaagc gctggtcgac gtgctgggcg
    15541 ctgcccgggt ggtggccggg tcgaccatga acagcgccgc caggctcggg ctcggccggg
    15601 tggcgcacac ccatctgggc aagtcggtgc tgggcgagct cgacgggcgg gcgagcgagc
    15661 gcctggagcg tctggcagcg ctcttcgacc ggggcggcct gccggttgca acgaccgaca
    15721 acatcatggg ccatgtctgg cagaaattcg tcctgaacgc ggccctcaac ccggtctgcg
    15781 ccatcacccg gctcaggccc ggcgaggtgg ccgagagcgc ttcggcctgt gccctggtcc
    15841 gcgacatcct gcgcgaggtg ctggcggtgg tggcggccga agggatcgac cttcccgatc
    15901 ccgatccgac ggagggcatc ctcggtcacg cggtcgggcg aatgaacaag ccctcgatgc
    15961 tgcagcatgt cgagcagggc cggcggaccg agatcgccgc gctgaacggg gcgctcctgc
    16021 cgatcgccgc gcggcacggc attccctgcc ccacgaaccg gacgatcgtg cagctcgtcg
    16081 aggcgatcca ggggcgggct gcgcgaacgg cataacgccg accaggattc gtcgctcgaa
    16141 tccgtttgcc atgccttgtc aaataggcgt tgacgccctt caatggcagt gaggcggtcg
    16201 ccgtcggatg ccgccagggg tggaagcggc acgatgtcct ttccgatcat cggcatcggc
    16261 tcgtcggctg gcggcctcga gccgctgcag gcgctgatcc gtgcggtgcc ggaggccagc
    16321 gggctggcgt tcgtcatcgt ggcgcatctc gatccgaccc agaaaagcca cctgcccgag
    16381 ctcctggcac gctgcaccgc gatgccggtc atgcaggtcg aggagacgac cgaggtgcgg
    16441 ccggaccatg tctatgtgat cgggccggac caggatctgt cgatcgaggg cggggccctg
    16501 cacaccaaga aatcggagct gccgcgcggg caccggcatc cggtcgatgt cttcttccgc
    16561 tcgctggcgg aagatcaggg tcggcgggcc atcggcatcg tgctctccgg caccggcacg
    16621 aacggctccc tgggcctgcg cgccatcaag gcgaaaggcg gcatcgcgat cgcccaactg
    16681 ccggagactg ccagcttcca gggcatgccg cgcagcgcca tcggcaccgg cgtggtcgat
    16741 ctggtcctgg cgccagagca gatgcccgag gcgctgctcg acctcgcccg gcacccttac
    16801 gtgcgggcgc ccaagaccac cgaggagcgg gcggccgacg actcgctgaa ggcggtcctg
    16861 tcggtggtga cggcccggac caggcgggac ttcaccggct atcgccagcg gaccctcgtg
    16921 cgacgcatcc atcgccgcat gggcctgctc aagatgaccg agctcagggc ctatgccgac
    16981 cggctgcgcc aggatccgga cgagaccagg gcgctggtgg ccgacatgac gatcaacgtc
    17041 accggcttct tccgcgatcc ggaggcctgg cagacgctgg cggagacggt catcgccccc
    17101 ctggtgcgcg agcgcgaccc gggcacggag atccgcgcct ggctgcccgg ctgctcgacc
    17161 ggcgaggagg cgtattcgct gggcatgctg atcgtcgagg aggccgagcg cgccggcaag
    17221 agcttcgacg tcaagatctt cgcgaccgac atcgccgagg gtgtcctcgg aacggcgcgc
    17281 ggcggtcact acccgggcag catcgcgctg gatgtcggcg aggagcggct gagccgcttc
    17341 ttcgtgcagg tggacgacag ctacacggtt cgcccggtcg tgcgcgagct ggtcaccttc
    17401 gcgccacaag atctgctgcg cgacccgccg ttctccaagc tcgacctggt gagctgccga
    17461 aacgtgctga tctatctcga gccggagcac cagaagaggc tcctcgccct cttccatttc
    17521 gcgctccgcc cggacggctg cctcttcctc ggctcgtccg agaacgtctc gggcctggag
    17581 gatcagttcc agccgatctc gaagaaatgg cggatctttc ggcgggccgg cccgacgccg
    17641 agcgagatcg tcgacttccc gctcggcgct ggccgcgggt cgcgagcagg cggtctcgat
    17701 acgctgcccg ccggtgttgg cgagctacgg gcgcgcgccg ccgatgtcat ggatcgcgcc
    17761 ctgctcgagc gccacgcgcc ggccagcgcg ctcgtcgacg agcgctatcg gatccatttc
    17821 atgcgcggcc cgatcgacga ttatctcaag ccgccggtcg gcgagcccac cttcgatctc
    17881 ctcgccatgg cgcgggaggg ccagcagatg gcgctgcgca cggcgatcag gcgggcgctg
    17941 gacgagaatc gcgagatcgt ggcgctcgcc cgcgtcaggc gtggcgggga gcggcggcac
    18001 gtccggctgg tcgtgacgcc gctgcggcgc ggcgcccagg cgccggcccg cctcctgatc
    18061 ggcttcttcg agcacgatcc cgcgaccgag aaggtcgagg ccgatgtccc cgagaggtcg
    18121 cccggcgaga gcctgatcca ggcggagctc gacaatgcgc gcgaggacct gcggttgtcg
    18181 gtcgagcaga tggaatcgac caacgaggag ctgaaggcgt cgaacgagga gatccgctcg
    18241 atcaacgagg agctgc
//
LOCUS       DAOTFI010000086        18222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3682_length_18222_cov_6.301811, whole genome shotgun sequence.
ACCESSION   DAOTFI010000086 DAOTFI010000000
VERSION     DAOTFI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18222
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3682_length_18222_cov_6.301811"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="P5558_10485"
     CDS             complement(<1..1018)
                     /locus_tag="P5558_10485"
                     /inference="COORDINATES: protein motif:HMM:NF017826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HRY24778.1"
                     /translation="MNAEHAQEAEVEAEVIDFDEMVASAPFAFPPEKSRPCWGVYDEP
                     VTDGGKRHRPGVYRHSMSKETKDTPPAPINTWISGPVHLEALTRNGEGQDFGVLLRFI
                     DREGNKRLWAMPYELLCADGADLRRELLSMGLAISPQHRAKLGEYLQHKTPKKRMLCT
                     SRTGWAGSGAFVLPDGVIGPDAGGVVFQDGGRIGRVHGVAGTVESWRNSVARLAIGNP
                     LMQFGMSAPFAAPLMDLTHTEGGGFNFVGSSSSGKTTILEAARSTFGGPDFKRSWRAT
                     SNGVEGVAEQHNDLILCMDEMSEVDPREAGAIAYMLTNGVGKSRATRTGASRPPKRWR
                     TLVFS"
     gene            complement(1015..1983)
                     /locus_tag="P5558_10490"
     CDS             complement(1015..1983)
                     /locus_tag="P5558_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24779.1"
                     /translation="MAKTISGGAVVRGYNAGKFYCTRTADVAGFADMRRVLEKAAGIP
                     YACIIRGEPLPGIDLENARRLSNRAEHGDAVTFVAKSRQWLGIDTDECPEPAGLDFST
                     DPEEGVEHVIGLLPDEFADSACWWQATGSAGIKAGINVRLWYWLSRPVSDGECKGWLH
                     HAPADRALYNPVQPHFVAAPLILSGPDPVARRFGVRQGLDDTVEVPAELPVIETVEPI
                     HVDVVLRDLSADEQRRIAATIKNSPVAREIWNSKRDYPDRSSRHFAFIGGLLRAGIHD
                     FEDWQELIAFAVVKLDQKLGVDTSKATRADYIGRTIGAVLARETAR"
     gene            complement(2035..2250)
                     /locus_tag="P5558_10495"
     CDS             complement(2035..2250)
                     /locus_tag="P5558_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24780.1"
                     /translation="MPKLLEDYIDRPTLAAELGVNWRTIHRYENQPDGLPSLLIGGRR
                     MYKLDSVRAWLERRERKPNPTRGRRGA"
     gene            complement(2367..3278)
                     /locus_tag="P5558_10500"
     CDS             complement(2367..3278)
                     /locus_tag="P5558_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24781.1"
                     /translation="MERDISWAEDFKPIDEFQAIHLVEVATGWSSKKAKKWLLDNVRS
                     DRVGTLLKEGWGIGETRESNHKRFKDAGALEQLHISARDLAAALDEFHNECETVGALL
                     SSPPLAGTSEAPRPIKLLDGLSARRRVEEAAQWKPEEAQVWLCHALVAGRVRGWHAAS
                     AKEAFTPELDPTEWPISFSESMPISENGGSLNFYLGKVCVDAESLSEALLTAFPRQEG
                     GAQEAATTVKRGRPPKDYWPRLYVEAGAWLAHNGGEDETGGKSALAEFLMTRATAHGS
                     GLERSQAFKKSKEILDAFRRLRSDGAL"
     gene            complement(3355..4545)
                     /locus_tag="P5558_10505"
     CDS             complement(3355..4545)
                     /locus_tag="P5558_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRY24782.1"
                     /translation="MRQRLTDSIIKALPAPASSNRITYDAELPGFGVRVTAAGARAFV
                     LNYRAGGRERRITIGQHPAWTVKAARDEAKRLRREIDSGRDPLAEEQAERAASTVAHL
                     CQEYTEHHLPKKRARSQADDRAMLEREVLPRLGRLKVADVTFTDIDRLHREITTGKKR
                     DRPAPIRANRVVALCSKMFALAIRRGWRADNPAKGIERNPENKRERFLAGDEVARLTA
                     ALAVHRDQQAADIIRVLLLTGARRGEVLSMRWADLDLEAGVWVKPSAHTKQKKAHRVP
                     LSAPARQLLTGIPQTSEYVFPGRSGGHRVEIKNDWATICKAAKLEGVRVHDLRHSYAS
                     FLASAGLSLPIIGALLGHTQAQTTARYAHLFDDPLRAATERVGAIVSPVEGGGEVVPM
                     KGRT"
     gene            5098..5715
                     /locus_tag="P5558_10510"
     CDS             5098..5715
                     /locus_tag="P5558_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24783.1"
                     /translation="MMDTNQQPKPPGSAGTQGVSSLKKTAADATTTAREHAERASDEL
                     RAQVSRGVDAAKDTVVEQSTMAKDTTAGELSRTARALHDAASKLDADDHGRSHVPQSL
                     LREAADGLDELAQSLQGRSVGEMVGDLSAFGRKNPVAFMGAAALAGFALGRFARASER
                     HAAPADYPAPRSQPTPGATRQPPVATASGAATKPFPAATGGTTHG"
     gene            5708..6097
                     /locus_tag="P5558_10515"
     CDS             5708..6097
                     /locus_tag="P5558_10515"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRY24784.1"
                     /translation="MADTIDRSASSLLGDLVSQVTELFRKEIMLLRAELGEKATQAVT
                     ACGLIVAAVVLALTALNVLAAALVVALENLGIPGGWAALIVGVILAAIAWALASKGIS
                     ALKASNLAPERAARAASRDAALVKGKM"
     gene            6097..7215
                     /locus_tag="P5558_10520"
     CDS             6097..7215
                     /locus_tag="P5558_10520"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HRY24785.1"
                     /translation="MAHHSTDPDGRSAAALEREVDAERAKVSQTIDALQSKMSIGNVV
                     DQVTEAVGAHGGEVARNLGRQMRDNPLPLLLTGIGLAWLMAGSGPRSADLDDDFDDDF
                     DDDLDNGRDLYPATTYPRRTVAGAERPLGTRPYDSGVSSHAGDPLRPHEHGGRDYADE
                     DEHGMGDKVRDAAGNVRDKASGLAESAGEFGRKVSGTLGDAASSVSDTASSMSDKARR
                     RADAARRAAADVGHAAARRGRRAGRGITHMMEEQPLVFGALALAIGAAVGGALPRTRT
                     EDEWVGGPSDRLKDAAGSMVKEEGRKLQATASAVVDEAGKIADEAAGEVDRRTPDGKS
                     FVEQAEDRLGKAADRLADRARDEADRQKLGQAAAPDRK"
     gene            7224..8165
                     /locus_tag="P5558_10525"
     CDS             7224..8165
                     /locus_tag="P5558_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRY24786.1"
                     /translation="MADDPPGRAARRPSHFGFADWKAILLRVYEQIGKDHLSIIAAGV
                     AFYAMLALVPAITAAFGLWGLFADPAQIAGQFAGLRSVVPEDAYTIIEEQMTALASTG
                     DRTLGVTSLLAILFALWTSRAGVAALMQGLNIVYDEKEERGIIASLAMSFLLTFLVIT
                     VGIVALAAIVVLPAVIGFLQLGGAIRWALLLLRWPILLGAMILVIGLLYRYGPNRANA
                     KLSWVSPGAVLATVIWLVASIAFSVYVSEFASYNKTYGTLGAIVILLMWMYISSFVVL
                     LGAELNAEMELHTTRDTTTGPERPMGERGAYVADNVV"
     gene            8244..9779
                     /locus_tag="P5558_10530"
     CDS             8244..9779
                     /locus_tag="P5558_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY24787.1"
                     /translation="MRKRVFVVALGESTFDLIEPWAAAGHLPVLASLMRGGTHGRMRS
                     QVPLMTPQLWGSIVTGRTPGHHGAFDFWQRSPKGAFHAIDGSALRCPTLWEMLSRRGV
                     PCGIVNLPFTYPPAAIDGFVIAGEDAPGAHRSIAHPPELYDEITEKFGRYRLKDIFPG
                     GRQKTDYLTLIDEDVAAQTEVFEHLFRTRPWRFGMAFFSATAIAQHYFWRDMAEATPG
                     DPFADVVLTAYRAVDRALGRLLEAVGPDTTVFVISDCGAGPLRSGVQINTILEQHGFL
                     ARKAAKPKAGRRLVAGARKQAQALLQRHLPDRLYYQVNHRLGGLKTKVQSYLAQSDLD
                     WPRTQVFSRGKEGDLFVNLKGRDPHGIVEPGADYEAVRDRLSETFMALRDPSTGEPAV
                     ERVYRREELFQGPMLEHAPDMVVLWKDTAYMPSEDDRNRDQVFVERWREYMNWPTSGS
                     HRVDAVLIAKGPGVAEGRTIMTARLIDLAPTWLHALGQDVPAELEGRVMQELFTAATV
                     PAA"
     gene            9776..10834
                     /locus_tag="P5558_10535"
     CDS             9776..10834
                     /locus_tag="P5558_10535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY24788.1"
                     /translation="MIHRPLGRTGLMVSAIGLGCQSLGGGLYHRDDAEALRLVRRAVE
                     LGVTFFDTADHYSQGRSETVLGRALAGLGDRVVIATKIGTSFPAGTRAALAMRDLVRP
                     VGTALRPLRKLLQRYRFRQGRSDFSADAIRRSVEASLGRLRVEAIDLLQFHKPDAATM
                     RAGMLFETAARLQAEGKVRHYGVACVDLDDALVAVEQPGVATVQIAINCLEPTGLDRL
                     TDAAAARGVAVIGRNPRAIGLLTDAHEETMGDASAYGPHHATRLAAARSLRGLIRPDR
                     TLAQAAIRHAVELPGVASVLPRAVTRAELDANLASLDVPPLTPEDQELIARAQAMTAP
                     LAGPIRQGSSASRKTAWT"
     gene            10831..11862
                     /locus_tag="P5558_10540"
     CDS             10831..11862
                     /locus_tag="P5558_10540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY24789.1"
                     /translation="MIYRTLGRTDLRVSAIGLGCQSLGGGLFHRDDSQSERVLAEAVE
                     RGVTFFDVSDHHSLGAAERLLGRCLGPVRDKVIITSKAGYDYAPLTRTALRLRDLVRP
                     ISAALKPARRSLHKLRLAQGRFSFDPDYIVRSVERSLERLRTDRLDLFQFYKPPAEAL
                     RDGRLYDAAERLKRQGKIRHYGVACLHIEDAMAALEHDGNATVQLAINCLEQKGVPEL
                     IDAAQARGVAVLARHPKAIGLLTNDHDDLMGDSSAYRHDQAERMRAARQLRALIREDR
                     TLAQAAIRFVLDMPGVDIVVPRAVTVDQLRENVGSIEVPPLSDSDRQCIEAVRRQTDG
                     LQGPVRHAA"
     gene            11864..13015
                     /locus_tag="P5558_10545"
     CDS             11864..13015
                     /locus_tag="P5558_10545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY24790.1"
                     /translation="MPAKRPLRLCLLCVEIFAFGKYGGFGRATRITGAALARRGVEVH
                     AIVPLRGGQRPVERLDGMTVHGYPPHRPDRIAGIARTIDADIHHSQHPSLATAIALAA
                     RPASRHVVTCRDPKSLRDWRIELARPSLGRAQVLANWLFEDGPGVAAAVRRMDGIFAA
                     SPHLVAEIARKYGLDADRIGLLPTPVRLPPEPVKAATPTVISVGRWDRRKRPERFIEL
                     ARAMPAIRFVAIGRSRDPAYEAELRARAAGIANLELAGFIDQFASDALERRLAAAWVL
                     VNTAGREGLPTAFLEALAHGCALLAEVDPGGITSRFGEAVADGDFRAGLERLLSGDAW
                     RAKGAAGRAHVAASFELDHVVDLHLSLYERLLATKPPRRWRPSPGARPA"
     gene            13012..14151
                     /locus_tag="P5558_10550"
     CDS             13012..14151
                     /locus_tag="P5558_10550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY24791.1"
                     /translation="MNILILCYEYPPLGGGGSRVVAGLARALAEAGHAVTIYTSRMPG
                     LPAEAFDGAVRVRRVRTWRRRADRSNAPELLVYAAAMLVAVLRDRPDRAADLAHVHFL
                     LPDGLVAWLMRRLTGLRFIATAHGSDVPGYNPHRFRLAHRLLAPLWRRIVASADRVVC
                     ASMGLRDSIVRAAPEAAPVVIPNGLDEARFSPDQPRRLALLSVTRLFERKGLHHLLDA
                     AAGLRRPVPIDIVGAGPQRAALEAQAARLGLPVTFHGWLDNDDQKLRDLLEKTAIFVL
                     PSSAENFPVSLLEAMAAGMAIVTTADTGCAEVVGDAALLVPSGDVPALGAALDRLIGD
                     PALRARLGARARRHFERAFTWTTVAATHERLYRQVLAERAKPADA"
     gene            14144..15124
                     /locus_tag="P5558_10555"
     CDS             14144..15124
                     /locus_tag="P5558_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRY24792.1"
                     /translation="MPDKIPWARLRIAVAALALGALFLAIPPATVLDAWRDARPGLLA
                     LGMAWMAAAAVASSAAFYATLRLRGIAPPLAEVVRTDIAGHFYTFTAPFGVVAGGAAR
                     VLRLQGPGRPMASLIGAILTNRLVNIWAAAALACLALVAVRPAIGATWPVWLLLLSGI
                     AGLALAAWLAAHHRRIRLLVAGRFVPLLPLAPQRRRRIRRTLRRLGGAGGTTSLPLHL
                     VPVALVVLRHLCSAAALAAFAAAFGLDLGFVASLWVRMALNVAMLVPLSPAGLGVREV
                     GFVVLLTPFAVPESDALAIALCVLAYQIVVAAIGGLCELTDSLRAARPAR"
     gene            complement(15491..17107)
                     /locus_tag="P5558_10560"
     CDS             complement(15491..17107)
                     /locus_tag="P5558_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRY24793.1"
                     /translation="MSERFDHIVVGGGASGCTVAGRLARAGARVLLLEAGLSNRHPLL
                     DMPPGIFKMINGSKFMRYHQTVPQAHLGGRVHDIPQGRVLGGGSSVNAQVYMRGRPSD
                     YDAWHEILRGNNDAVGWSWQDVLPHFRAMEGNNRLNDERHGTSGPLLVSDPGHINEVS
                     RWFVQAVQGLGEPFNPDFNGPSQRGVGFYQFTNRNGRRSSAAYAFLTPEEQNPALTVR
                     LEAEVRKVEIEGGRAVGVTWRDKAGAMVTAHADGEVILAAGALATPQLMMLSGLGPAD
                     HLVEHGIECIADLPGVGQNLIDHPEVPMVANANGPYGYYRQGVGWRMLWNGLQLKLFG
                     SGPVTSAGVEAGAFVNPTDHEAPPTIQAFCVPIVYLDRDTLGLVEDGYGLTITTVVVK
                     PRSRGELRLRSADPAAMPLVSPNLLKDEADMAAMVAGQRFFLRAFAESPLRERIARVA
                     IPDPADASDAALAAHCRRFVKTNYHPAGTARMGADGDRMAVLDARLRVRGVDGLRVCD
                     MSAVPDINAGNTNAPAMMLGHRCAELVLGG"
     gene            complement(17104..17841)
                     /locus_tag="P5558_10565"
     CDS             complement(17104..17841)
                     /locus_tag="P5558_10565"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY24794.1"
                     /translation="MDARISSGRRAADAIVERLELRIVSGELPDRTALPPERVLMAEF
                     GASRTVIREAITALSSRGLIENRPRYRPIVRKPGFDAALGALGGIVGHLLGEAEGVKA
                     LFDSRIFLERALVREAAMHARRADIQALREALAANEAAIPDSARFYATDVAFHAVLYE
                     IPRNPIMPAIHRAYVAWLAVHWHRMLRSPERNRVNYQSHRTILEAILERDPDAAEAAL
                     ASHLNAAWEYVRVTFEPAPERTEAGQP"
BASE COUNT         2703 a         6730 c         5915 g         2874 t
ORIGIN      
        1 tcgagaacac gagcgttcgc cagcgcttcg gcggtcggct ggcgcctgtc ctggtagccc
       61 tggatttgcc aacgccgttg gtgagcatgt acgcgattgc tcccgcctcg cgcgggtcga
      121 cctcgctcat ttcgtccatg cacaagatca ggtcgttgtg ctgctcggcc acgccctcga
      181 caccgttcga tgtggcccgc cacgagcgtt tgaaatccgg cccgccgaag gtggatcgtg
      241 cggcctccag gatggtggtc ttgcccgagc tggagctgcc gacgaaattg aagccaccgc
      301 cttctgtgtg cgtcaggtcc atcagcggtg cggcgaatgg cgccgacatc ccgaactgca
      361 tgagcgggtt gccgatcgcg agccgggcca cgctgttgcg ccagctctcg accgtgccgg
      421 ccacgccgtg cactctgccg atcctgccac cgtcctggaa gacgacgccg ccggcatctg
      481 gcccgatgac gccatcgggc aggacgaacg cgcccgaccc tgcccacccg gtccggctgg
      541 tacagagcat ccgttttttc ggggtcttgt gctgaaggta ttcgcccagc ttggcacgat
      601 gctgcgggct gatcgcgagc cccatggaca gcagctcgcg ccgcaagtcc gcgccgtcgg
      661 cgcacagcag ttcgtaaggc attgcccaca gccgcttgtt gccctcgcga tcgatgaagc
      721 ggagcagcac gccgaaatcc tggccctcgc cgttgcgggt aagcgcctcg aggtggacgg
      781 gtccgctaat ccatgtgttg ataggcgccg gcggtgtgtc cttcgtctct ttcgacatgg
      841 aatgccggta gacacccggc cggtggcgct tgccgccgtc agtgaccggc tcgtcataga
      901 caccccagca tggccgcgac ttctcgggcg ggaaggcgaa cggtgccgat gccaccattt
      961 cgtcgaagtc gatgacctcg gcctcgactt cggcctcttg tgcatgctcg gcattcatcg
     1021 tgccgtctcc cgtgcgagaa cagccccgat ggttcggccg atgtagtccg ctcgggtcgc
     1081 cttactggtg tcgacgccga gcttctggtc gagcttgacc actgcgaagg cgatcaactc
     1141 ctgccaatcc tcgaaatcgt gaatgccggc ccgaagcaag ccgccgatga acgcgaaatg
     1201 tcggctggaa cggtcgggat aatcccgttt gctgttccat atctctcggg ccacgggcga
     1261 gttcttgatt gtcgccgcga tgcgccgctg ttcgtccgcg cttaggtccc gcaacaccac
     1321 gtcgacatga atgggttcga ccgtctcgat aaccggcagc tcggccggca cctcgaccgt
     1381 gtcgtcgagt ccctgccgca cgccgaaccg tcgtgccacc gggtcgggtc cgctcaggat
     1441 gagcggtgca gccacaaagt gcggctgaac cgggttgtag agcgcccggt cggctggcgc
     1501 gtgatgcaac cagcctttgc actcgccgtc gcttaccggc cggctgagcc agtaccacag
     1561 ccgcacgttg atgccggcct tgatgcccgc agagcccgtg gcctgccacc agcacgccga
     1621 atcggcgaac tcgtcaggca aaagcccgat gacgtgctcg acgccctcct cagggtcagt
     1681 cgagaagtcc agcccggccg gctccgggca ctcgtcagtg tcgatgccca gccattgccg
     1741 agacttggcc acgaacgtca ccgcgtcgcc gtgctctgcc cgattgctga gccgccgcgc
     1801 gttctccagg tcgatgccgg gcaacggctc gccgcggatg atgcatgcgt aggggatgcc
     1861 cgcggccttt tcgagcaccc gcctcatatc ggcgaagccg gccacgtcgg cagtgcgggt
     1921 gcagtagaac ttgccggcat tgtagccccg gacgacggct ccgccggaga tggtcttcgc
     1981 catgaccccg gagcgggcta gtagaatggt catggtgtcg gcggccatcg ccgatcaggc
     2041 cccacgccga ccgcgggtgg ggtttggctt gcgctcgcgg cgctcaagcc atgcccgcac
     2101 actatcgagc ttgtacatgc gccgaccgcc gatcagcagc gacggcaggc cgtccggctg
     2161 attttcatag cggtggatgg tacgccagtt gacgcccaat tccgcggcga gcgtcgggcg
     2221 gtcgatatag tcttcgagaa gcttgggcac ttggcgttgt cctgtctttg gggggacacc
     2281 gcctgccaat attcacatat ttttccgcac gtcgtccgat tagtccgaaa atgaattatc
     2341 ggactcttgg aaaatttcag tacgactcaa agcgcgccgt cgctccgtaa gcggcgaaag
     2401 gcgtcgagga tctccttgga ctttttgaag gcttggcttc gctcgagccc cgacccatgc
     2461 gcggttgcgc gggtcatgag aaactccgcc agcgccgatt ttccaccggt ctcgtcttca
     2521 cccccgttgt gggccagcca cgcacctgct tcgacgtata gtctgggcca ataatccttc
     2581 gggggtcgtc cgcgcttgac cgtcgtggct gcctcctgcg caccgccctc ttgccgggga
     2641 aacgcggtga gcagggcttc actcaagctt tccgcatcga cgcagacttt cccaagatag
     2701 aaatttagcg agccgccatt ttcgcttatc ggcatgctct ccgagaagga gatcggccac
     2761 tcggtcgggt cgagttctgg cgtgaatgct tccttcgcac tcgctgcgtg ccaccctctc
     2821 actcgcccgg cgacaagcgc atggcacagc catacttgcg cctcttctgg cttccactgg
     2881 gccgcttcct cgacgcgtcg gcgcgcgctc aggccgtcaa gcagcttgat gggccggggc
     2941 gcttcagacg tgccggccaa aggcgggctg gacaacaggg cgccgaccgt ttcgcactca
     3001 ttgtggaact catcgagtgc cgcagccaaa tcccgggcgc ttatgtgcag ttgttctaat
     3061 gcgccagcat ccttgaagcg cttatgattg ctctcccttg tttctccaat tccccaccct
     3121 tcttttagga gcgttcccac gcgatcagag cgaacattat cgagcaacca ctttttcgcc
     3181 tttttcgaag accagccggt agcaacctcc actaagtgaa tcgcctgaaa ctcgtcaatc
     3241 ggcttgaagt cttccgccca gctgatgtcg cgctccatgc cttcgggcct ctccttgcgg
     3301 caaggatgtc ctgggacgcc ccggcagcga cccatttctg ctcgtgccga taccttaagt
     3361 cctgcctttc atcggcacca cctcgccgcc gccctcgacg ggagacacga tggccccaac
     3421 gcgctcggta gcggcccgca gcgggtcgtc gaacaggtgt gcatatcgcg ctgtcgtctg
     3481 cgcctgcgtg tgccctagca gcgcgccgat gatgggcagg gaaagaccgg cgctggcgag
     3541 aaacgaggcg taggaatgcc gcagatcgtg aacgcggacg ccctccagct ttgccgcttt
     3601 gcagatcgtg gcccagtcgt ttttgatctc gacgcgatgc ccgccgctac ggccgggaaa
     3661 gacgtactcg ctcgtctgcg ggatgccggt gagcaactgc cgagccggtg cggacagcgg
     3721 tacgcgatgc gccttcttct gcttggtgtg cgcgctcggc ttcacccaaa ctccggcctc
     3781 aaggtccaga tcggcccaac gcatggacaa cacctcgcca cggcgagcgc cggttagcag
     3841 cagcactcga atgatatcgg ccgcctgttg gtcgcggtgc acggccaggg cagcggtcag
     3901 ccgggcaacc tcatcacccg cgagaaagcg ctcgcgcttg ttctcggggt tgcgctcgat
     3961 gcccttggcc gggttgtccg cccgccagcc tcgacggatc gcgagcgcga acatcttgga
     4021 gcacaacgcg acaacgcggt ttgcccgaat cggcgccggc cgatcccgct tcttgccggt
     4081 cgtgatctcc cggtgcagcc ggtcgatatc ggtgaacgtg acgtccgcca ccttgagccg
     4141 gccgagccgg ggcagaactt ccctctccaa cattgcgcgg tcgtcggcct gggagcgcgc
     4201 ccgctttttc ggcaggtggt gctctgtgta ttcctggcac aggtgcgcca cggtcgacgc
     4261 cgcacgctcg gcctgctcct cggccagcgg gtcgcgcccg ctgtcgatct cgcgccgcag
     4321 ccgcttggcc tcgtcacgag cggccttgac ggtccaggcg ggatgctggc cgatggtaat
     4381 ccgccgctct cggccaccgg cccgataatt gagcacgaag gcccgagcac cggctgccgt
     4441 cacccgaacg ccgaagcccg gcagctccgc atcgtaggtg attcgattgc tcgatgccgg
     4501 tgcgggcagg gccttgatga tgctgtcggt tagccgctgt cgcattgtct gtcggtcctc
     4561 gtgtccgtac cgtgtccgcc aaaaaggcgg cggaccgtga cagagtgttg acatgccgtg
     4621 tcagcgagtg tcaaggtaaa gtactgttag ataaagggtt cagcggcgga cacgagagat
     4681 ttcaatggct tgcacgatag cttgcggcag actcaaaatc tgttgccttc acgggcgtgc
     4741 tggttcgagt ccggccaggg gcaccaagca aacgagaggg tcgccggcgg cggcatcctc
     4801 cgccgcgagc cgacctcagc ccggctgggc cgcgctcctc ccccgagcca tcatgcaaga
     4861 agaacgagcc gggtcagcag tgtcgccgcg acttgcaccg gcgagacagc ggcatttccg
     4921 gcaacgggac acggaaccct tacccacgga caacgttctc tccacccgag cagtggagag
     4981 tgcaatgtac aatgttttct atgtgatcgg ggtgatcgtc gtggtgctgg cgctgctctc
     5041 gctcgtgggg ctcgcatgag cctctcgcca cgcgcatcct cagaaccagg gaaacccatg
     5101 atggacacga accagcagcc gaagccgccg ggctcggcag ggacgcaggg cgtctcgagc
     5161 ctgaagaaga ctgcggcgga cgcgacgacg accgcgaggg agcatgccga gcgcgcgagc
     5221 gacgagcttc gcgcacaggt gagtcgtggt gtcgacgcgg cgaaggacac ggtcgtcgag
     5281 cagtcgacga tggcgaagga cacgaccgcc ggcgagctct ctcgcacggc ccgggcgctg
     5341 cacgatgcgg ccagcaagct cgatgcggac gaccacggtc gctcccacgt gccgcagagc
     5401 ctgttgcgcg aggccgcgga cgggctcgac gagctggcgc aatcgctgca gggcaggtcg
     5461 gtcggcgaga tggtcggcga cctctcggcg ttcgggcgga agaaccccgt cgcgttcatg
     5521 ggtgcggcag ccctcgccgg cttcgcgctc ggccgcttcg cccgcgcctc cgagcggcac
     5581 gcagcgccgg ccgactaccc ggccccgcgg tcgcagccga ctccgggagc gacccggcag
     5641 ccacccgtcg caacggcctc gggggctgca acgaagcctt tcccggccgc gacaggaggg
     5701 accacccatg gctgacacga tcgatcgatc ggcttccagc ctgctcggcg acctcgtcag
     5761 ccaggtgacg gagctgttcc gcaaggagat catgctgcta cgcgccgagc tcggcgagaa
     5821 ggcgacacag gcggtcaccg cctgcggcct gatcgtggcg gccgtggtgc tcgccctgac
     5881 cgccctgaat gtcctggcgg cggcgctcgt cgtcgccctc gagaatctcg gcatccccgg
     5941 tggctgggcg gcgctcatcg tcggcgtgat cctggcggcg atcgcctggg cgctggcgag
     6001 caaaggcatc tcggcgctca aggccagcaa cctcgcaccg gagcgggccg cccgtgcggc
     6061 gagccgcgat gcggcgctcg tgaaaggaaa gatgtgatgg cgcaccattc gactgatccg
     6121 gacggccgca gtgccgcagc cctcgaacgc gaggtggatg ccgagcgggc gaaggtctcc
     6181 cagacgatcg acgcgctgca gagcaagatg tcgatcggca atgtcgtcga ccaggtcacc
     6241 gaggcggtcg gcgcgcatgg cggcgaggtt gcccgcaatc tcggccggca gatgcgcgac
     6301 aacccgctgc cgctcctcct gaccggcatc gggctcgcct ggctgatggc gggcagcggc
     6361 ccgcgcagcg ccgacctcga cgacgatttc gatgacgact tcgacgatga cctcgacaat
     6421 ggcagggacc tctaccccgc cacgacctac ccgcggcgga cggtggccgg cgccgaacgg
     6481 cctttgggca cgcgccccta cgactcgggc gtttcctccc atgccggcga ccctctccgc
     6541 ccgcacgagc atggcggccg cgactacgcc gacgaggacg agcacggcat gggcgacaag
     6601 gtcagggacg ccgccggcaa cgtccgggac aaggcgtcgg ggctggccga atcggccggc
     6661 gagtttggtc gcaaggtgtc cggcacgctc ggcgatgccg cctcgtccgt ctccgacacg
     6721 gcctcgtcca tgtcggacaa ggcgcgcagg cgtgcggatg cagcgcggcg cgccgccgcc
     6781 gatgtcggcc atgcagccgc ccggcgcggg cgccgtgccg gccggggcat cacgcacatg
     6841 atggaggagc agccgctcgt cttcggtgcg ctggccctgg cgatcggtgc cgccgtgggc
     6901 ggtgccctgc cgcgcacccg caccgaggac gagtgggtcg gcggcccgag cgaccggctg
     6961 aaggacgccg ccggcagcat ggtgaaggag gaaggccgaa agctgcaggc caccgcctcc
     7021 gccgtcgtcg acgaggccgg caagatcgcc gacgaggcgg cgggcgaggt cgaccggcgg
     7081 acgccggacg gcaaatcgtt cgtcgagcag gccgaggatc ggctgggcaa ggcggccgac
     7141 cggctcgccg accgcgccag ggacgaagcc gatcggcaaa agctcggtca ggcggctgct
     7201 cccgaccgca agtgagcgac cccgtggccg acgacccccc ggggagggcc gccaggcggc
     7261 cctcccactt cggcttcgcc gactggaagg cgatcctgct ccgcgtctat gagcagatcg
     7321 gcaaggatca cctctcgatc atcgcggccg gcgtcgcctt ctacgcgatg ctggccctcg
     7381 tgccggccat caccgcggcc ttcggcctct gggggctctt cgccgatccg gcccagatcg
     7441 ccgggcagtt tgccgggctc cgctcggtcg tcccggagga tgcctacacg atcatcgagg
     7501 agcagatgac cgccctcgcc agcaccggcg accggacgct cggcgtcacc tcgctgctgg
     7561 cgatcctctt cgcgctctgg acctcgcgcg ccggcgtcgc cgccctgatg caggggctga
     7621 acatcgtcta cgacgagaag gaggagcgcg gcatcatcgc cagcctcgcc atgtcgttcc
     7681 tcttgacctt cctcgtcatc accgtcggca tcgtggcgct tgccgccatc gtcgtgctgc
     7741 cggcggtcat cggcttcctc cagctcggcg gcgccatccg gtgggcgctc ctgctgctgc
     7801 gctggccgat cctcctgggc gccatgatcc tcgtcatcgg cctcctctac cggtacggcc
     7861 ccaaccgcgc caatgcgaag ctctcctggg tctcccccgg cgccgtcctc gccacggtga
     7921 tctggctggt cgcctcgatc gcgttctccg tctacgtcag cgagttcgcg agctacaaca
     7981 agacctacgg caccctcggc gccatcgtca tcctgctcat gtggatgtac atcagctcgt
     8041 tcgtcgtcct cctcggcgcc gagctgaatg ccgagatgga gctgcacacc accagggaca
     8101 cgaccaccgg ccccgagcgg ccgatgggcg agcgcggcgc ctatgtggcg gacaatgtcg
     8161 tctgaccggc ccgatccggg gcgaattgtt gagcaaacgg gggtatagag tccggcgtcc
     8221 ggaaaacgcc ggagggaact ggcttgcgca agcgtgtctt cgtcgtggct ctcggcgaat
     8281 cgaccttcga cctgatcgag ccctgggcgg cggcgggcca cctgccggtg ctggcttcgc
     8341 tgatgcgggg cggcacgcac gggcggatgc gctcgcaggt gccgctcatg accccgcagc
     8401 tctggggctc gatcgtgacc ggccgcacgc ccggccatca cggcgccttc gatttctggc
     8461 agcgcagccc gaaaggcgcg ttccacgcca tcgacggctc ggccctgcgc tgcccgacgc
     8521 tctgggagat gctgagccgg cgcggcgtgc cctgcggcat cgtcaacctg cccttcacct
     8581 acccgcccgc cgccatcgac ggcttcgtca tcgccggcga ggacgcgccc ggcgcccacc
     8641 gctcgatcgc ccatccgccc gagctctacg acgagatcac cgagaagttc ggccgctacc
     8701 gcctgaagga catcttcccg ggcggccgcc agaagaccga ctacctgacc ctgatcgacg
     8761 aggacgtggc cgcccagacg gaggtcttcg agcacctctt ccgcacccgc ccctggcggt
     8821 tcggcatggc gttcttcagc gccaccgcca tcgcccagca ctacttctgg cgcgacatgg
     8881 cagaagccac gcccggcgac cccttcgccg acgtggtcct caccgcctat cgcgccgtcg
     8941 accgcgccct cggccggctg ctggaggcgg tcggccccga caccaccgtc ttcgtgatct
     9001 ccgactgcgg cgccgggccg ctgagatcgg gcgtgcagat caacaccatc ctcgagcagc
     9061 acggcttcct cgcccgcaag gcggcgaagc cgaaggccgg ccgcaggctc gtcgccggtg
     9121 ctcgcaagca ggcgcaggcg ctgctgcagc gccatctgcc ggaccgtctc tactaccagg
     9181 tcaatcaccg cctcggcggg ctcaagacga aggtgcagtc ctacctcgcc cagagcgacc
     9241 tcgactggcc acgcacgcag gtcttcagcc gtggcaagga aggcgacctc ttcgtcaacc
     9301 tgaagggccg cgacccgcac ggcatcgtcg agcccggcgc cgactacgag gccgtccgcg
     9361 accggctcag cgaaaccttc atggccctgc gcgacccttc caccggcgag cccgccgtcg
     9421 agcgcgtcta tcgccgcgag gagctcttcc aaggcccgat gctcgagcac gcgcccgaca
     9481 tggtcgtcct gtggaaggac accgcctaca tgccgagcga ggacgaccgc aaccgcgacc
     9541 aggtcttcgt cgagcgctgg cgcgagtaca tgaactggcc gaccagcggc agccatcgcg
     9601 tcgatgcggt gctcatcgcc aaaggcccgg gcgtcgccga gggccggacc atcatgaccg
     9661 cccggctgat cgacctcgcc cccacctggc tgcacgcgct ggggcaggac gtgccggccg
     9721 agctggaagg ccgggtgatg caggagctct tcaccgccgc caccgtgccc gccgcatgat
     9781 ccaccgcccc ctcggccgga ccggcctcat ggtctccgcc atcggcctcg gctgccagtc
     9841 gctcggcggc ggcctctatc atcgcgacga cgccgaagcg ctgcggctgg tccgccgcgc
     9901 ggtcgagctc ggcgtcacct tcttcgacac ggcggaccac tactcgcaag gccgctcgga
     9961 gacggtgctg ggccgggccc tcgccggcct gggtgaccgg gtcgtcatcg ccaccaagat
    10021 cggcaccagc tttcccgccg gcacccgcgc cgcgctggcc atgcgcgacc tcgtccggcc
    10081 cgtgggcacg gcgcttcggc cgctcaggaa gctcttgcag cgctaccgct tccggcaggg
    10141 ccgctcggat ttctcggccg acgccatccg acgatcggtc gaggcgagcc tcggccgcct
    10201 gcgggtcgag gccatcgacc tcctgcagtt ccacaagccc gacgccgcga cgatgcgcgc
    10261 gggcatgctc ttcgaaaccg ccgcacggct gcaggccgag ggcaaggtcc ggcactacgg
    10321 cgtcgcctgc gtcgatctcg acgacgcgct ggtcgcggtg gagcagccgg gcgtcgccac
    10381 ggtgcagatc gccatcaact gcctcgagcc caccgggctg gaccggctga ccgacgcggc
    10441 cgcggcccgg ggtgtcgccg tcatcggccg caacccacgc gccatcggcc tgctcaccga
    10501 tgcccacgag gagacgatgg gcgacgcctc ggcctacggc ccgcaccacg ccacccgcct
    10561 cgccgccgcc cgctcgctgc gcggcctcat ccggcccgac cggaccctgg cccaggccgc
    10621 catccgccat gccgtcgagc tccccggcgt cgccagcgtc ctgccgcgcg cggtcacgag
    10681 agccgagctc gatgccaacc tcgccagcct cgatgtcccg ccgctgacgc ccgaggacca
    10741 agagctcatc gcccgcgccc aggccatgac cgccccgctc gcggggccga tccgccaggg
    10801 cagctccgcg agcagaaaga ccgcctggac atgatctatc gcaccctcgg ccgcaccgat
    10861 cttcgcgttt ccgccatcgg cctcggctgc cagtcgctcg gcggcgggct ttttcaccgc
    10921 gacgacagcc agtccgagcg cgtgctcgcc gaagcggtcg agcgaggcgt caccttcttc
    10981 gacgtgtcgg accaccattc gctcggcgcc gccgaacggc tcctgggccg ctgcctcgga
    11041 ccggtgcgcg acaaggtcat catcacctcc aaggccggct acgactacgc gccgctgacc
    11101 cgcacggcgc tgcggctgcg cgatctcgtc cggccgatca gcgccgcgct gaagccggcc
    11161 aggaggtcgc tgcacaagct gcgcctcgcc cagggccgct tcagcttcga tcccgactac
    11221 atcgtccgct cggtcgagcg cagcctcgag cgcctgcgca cggaccggct cgatctcttc
    11281 cagttctaca agccccccgc ggaggcgctg cgggacggcc ggctctacga tgcggcggaa
    11341 aggctcaagc gacaggggaa aatccgccat tacggcgtcg cctgcctgca tatcgaggac
    11401 gcgatggcgg cgctcgagca tgacggcaac gccaccgtcc agctcgccat caactgcctc
    11461 gagcagaagg gcgtgccgga gctgatcgac gcggcgcagg cccgaggcgt cgcggtcctc
    11521 gcccgccatc ccaaggcgat cggcctcttg accaacgatc acgacgacct gatgggcgac
    11581 agctccgcct atcggcacga ccaggccgag cgcatgcggg ccgcacgtca gctccgggcg
    11641 ctcatccgcg aggaccgcac cctcgcccag gccgccatcc gcttcgtgct cgacatgccg
    11701 ggcgtcgaca tcgtcgtgcc gcgcgccgtc accgtcgacc agctgcgcga gaacgtcggc
    11761 agcatcgagg tgccgcccct gagcgacagc gaccggcaat gcatcgaggc ggtccgccgc
    11821 cagaccgatg ggctccaggg cccggtccgg cacgccgctt gagatgcccg ccaagcggcc
    11881 gctccgcctc tgcctgctct gcgtcgagat cttcgccttc ggcaaatatg gcgggttcgg
    11941 ccgggcgaca cggatcaccg gcgccgccct cgcccggcgc ggcgtcgagg tgcacgccat
    12001 cgtgccgctg cgcggtggcc agcgccccgt ggagcgtctc gacgggatga cggtgcacgg
    12061 ctacccgccg caccgccccg accggatcgc cggcatcgcg cgcacgatcg atgccgacat
    12121 ccaccacagc cagcacccct cgctcgccac cgccatcgcc ctcgccgccc ggccggccag
    12181 ccggcacgtc gtcacctgcc gcgaccccaa gtcgctgcgc gactggcgga tcgagctcgc
    12241 ccggccgtcg ctcggccgcg cccaggtcct ggccaactgg ctcttcgagg acggccccgg
    12301 cgtcgccgcc gccgtccgcc gcatggacgg catcttcgcc gcctcgccgc acctcgtggc
    12361 ggaaatcgcg cgcaagtacg gtctcgacgc ggaccggatc ggcctcctgc cgacacccgt
    12421 gcggctgccg cccgagccgg tcaaggctgc cacaccgacc gtgatctcgg tcggccgctg
    12481 ggatcgccgc aagcggcccg agcgcttcat cgagctcgcc cgggccatgc ccgccatccg
    12541 cttcgtcgcc atcggccgct cgcgcgaccc ggcctacgaa gcagagctgc gcgcccgcgc
    12601 cgccggcatc gccaatctcg agctcgccgg cttcatcgac cagttcgcca gcgacgccct
    12661 cgagcgccgg ctcgcggcgg cctgggtgct cgtcaacacc gccgggcgcg agggcctgcc
    12721 gaccgccttc ctcgaagccc tcgcccacgg ctgcgccctt ctcgccgagg tcgaccccgg
    12781 cggcatcacc agccgcttcg gcgaggcggt cgcggacggc gacttcaggg ccggcctcga
    12841 gcggctgctg agtggcgatg cctggcgcgc caagggcgcc gccggccgcg cgcacgtcgc
    12901 cgccagcttc gagctcgacc acgtcgtcga ccttcatctg tcgctctacg agcggcttct
    12961 cgcaacgaag ccgccacggc gctggcgccc gtcgcccggg gcgaggccgg cttgaacatc
    13021 ctcatcctct gctacgaata cccgccgctc ggcggcggcg gcagccgggt ggtcgccggc
    13081 ctcgcccgcg ccctcgccga ggccggccac gccgtgacga tctacaccag ccgcatgccc
    13141 ggcctgccgg cggaggcgtt cgacggcgcc gtgcgggtgc gccgggtccg gacctggcgg
    13201 cgccgggccg accggagcaa cgcgccggag ctcctcgtct acgccgccgc catgctcgtg
    13261 gccgtcctgc gcgaccgccc cgaccgtgct gccgatctcg cccatgtcca tttcctcctg
    13321 cccgacgggc tcgtcgcctg gctcatgcgc cggctgaccg gcctgcgctt catcgccacc
    13381 gcgcacggct ccgacgtgcc gggctacaac ccgcatcgct tcaggctcgc ccaccgcctg
    13441 ctcgccccgc tctggcgccg catcgtcgct tccgccgacc gcgtcgtctg cgcctccatg
    13501 ggcctcaggg actcgatcgt gcgggccgca cccgaagccg ccccggtcgt catccccaac
    13561 gggctggacg aggcccgctt cagccccgac cagccgaggc gcttggccct gctcagcgtc
    13621 acccgcctct tcgagcgcaa gggcctccat cacctcctcg acgccgcagc ggggctccgg
    13681 cggccggtgc cgatcgacat cgtcggcgcg gggccgcaaa gggccgccct cgaggcgcag
    13741 gccgcccgcc tcggcctgcc cgtgaccttc cacggctggc tcgacaacga cgaccaaaag
    13801 ctccgcgacc tgctcgagaa gaccgccatc ttcgtcttgc cctcgagcgc cgagaacttc
    13861 ccggtctcgc tgctcgaggc gatggccgcc ggcatggcga tcgtcaccac cgccgatacc
    13921 ggctgtgccg aggtggtggg cgacgcggcg ctcctggtgc cgagcggcga cgtgccggcg
    13981 ctcggcgccg ccctcgaccg gctgatcggc gacccggcgc tccgcgcccg gctcggcgcc
    14041 cgggcgcgcc ggcatttcga gcgcgccttc acctggacga cggtggcggc gacgcacgag
    14101 cggctctatc gccaggtcct ggccgaacgg gcgaagcctg ccgatgcctg acaaaatccc
    14161 ctgggcgagg ctccggatcg cggtcgcggc cctggccttg ggcgccctct tcctcgcgat
    14221 cccgcccgcg acggtgctcg acgcctggcg cgatgcccgg ccgggcctcc tcgccctcgg
    14281 catggcctgg atggcggccg ccgccgtggc cagcagcgct gccttctacg ccacgctccg
    14341 cctgcgcggc atcgccccgc cgctcgcgga agtcgtccgc accgacatcg ccggccattt
    14401 ctacaccttc accgccccct tcggcgtggt cgccggcggc gccgcccgcg tcctcaggct
    14461 ccaggggccc ggccggccaa tggcctcgct gatcggcgcc atcctcacca atcgcctggt
    14521 caacatctgg gccgccgccg ccctcgcctg cctcgccctc gtcgcggtcc ggcccgccat
    14581 cggcgccacc tggccggtct ggctcctgct gctgtccggc atcgccggac tggcgctcgc
    14641 cgcctggctc gccgcccatc accggcgcat ccgcctcttg gtcgccggcc ggttcgtccc
    14701 gctcctgccc ttggcgccgc agcgccggcg ccggatccgc cgcacgctcc gccgactcgg
    14761 cggggccggc ggcacgacca gcctccctct gcacctggtc ccggtggcgc tggtcgtcct
    14821 gcgccatctc tgcagcgctg ccgccctcgc ggccttcgcc gccgccttcg gcctggacct
    14881 cggcttcgtc gcgagcctct gggtgcgcat ggcgctgaac gtcgccatgc tcgtcccgct
    14941 ctcgccggcg ggactgggcg tgcgcgaggt cggcttcgtc gtccttctga cgcccttcgc
    15001 cgtgcccgag agcgacgcgc tcgccatcgc cctctgcgtg ctggcctatc agatcgtcgt
    15061 cgccgccatc ggcggcctct gcgagctgac ggactcgctc agggccgcca ggccggcaag
    15121 gtgagcgata cgacgtgccc gcccgccccg gttgaagaaa gcggcaagct tggtgcggat
    15181 gacgcgagac gaacgagcac cgatacccgg acaccgtgcc ggaaacgcga gcagcgcata
    15241 tcttccgcga gcaggtgcag agaatccatg atcgctcgtg aaacccctgg tctcatcctg
    15301 atcaatgctt ttacccgatt ggcgcggaat caacttcagg ctatgatgat cattgttccg
    15361 aaatcggtgg ccggcccgca gcgccggctg aggagcactg atgcggatcg aggatcttcc
    15421 cggctggaca gcggcggcag cgctgatggc catgatcgtg atcgcggccc tgacctagca
    15481 ggcccccggt tcagccgccc aggaccaatt cggcgcagcg atgaccgagc atcatcgccg
    15541 gcgcattggt gttgcccgca ttgatgtccg gcacggccga catgtcgcag acccgaagcc
    15601 cgtcgacgcc gcgcacccga agccgcgcat cgagcaccgc catgcggtcg ccatccgccc
    15661 ccatccgcgc cgtgccggcc gggtggtagt tggtcttgac gaagcgccgg cagtgcgccg
    15721 ccaacgccgc gtcgctggca tcggccggat cggggatggc gaccctggcg atccgctccc
    15781 gaagcgggct ttcggcgaag gcgcgcagga agaagcgctg gcccgcgacc atggccgcca
    15841 tgtcggcctc gtccttcagg aggttgggcg agaccagcgg catggccgcc ggatcagcgc
    15901 tcctgaggcg caattcgccg cgcgagcgcg gcttgaccac gaccgtcgtg atggtcagcc
    15961 cgtagccgtc ctcgaccagg cccagcgtgt cccggtcgag atagacgatg ggcacgcaga
    16021 aagcctggat ggtcggcggc gcctcgtggt cggtcgggtt gacgaaggcc ccggcctcga
    16081 ccccggccga ggtgaccggc cccgacccga agagcttgag ctgcagcccg ttccagagca
    16141 tccgccaccc cacgccctgg cggtagtagc cgtagggccc gttggcattg gcgaccatcg
    16201 gcacctcggg gtggtcgatc aggttctgcc cgaccccggg caggtccgcg atacactcga
    16261 tgccgtgctc caccaggtga tccgcggggc cgagccccga gagcatcatg agctgcggcg
    16321 tcgccagcgc cccggcggcg aggatcacct cgccatccgc atgggcggtg accatcgccc
    16381 cggccttgtc acgccaggtg acgccgaccg cccggccccc ctcgatctcg accttccgca
    16441 cctccgcctc gagccggacg gtgagcgccg ggttctgctc ctccggcgtg aggaaggcat
    16501 aggccgcgga gctccgccgg ccgttcctgt tggtgaactg gtagaagccg acgccgcgct
    16561 ggctcggccc gttgaagtcc gggttgaagg gctcgcccag cccctgcacc gcctggacga
    16621 accagcgcga gacctcgttg atgtgcccgg gatcggagac caggagcggc cccgatgtgc
    16681 cgtgccgctc gtcgttcagc cgattgttgc cctccatcgc ccgaaaatgc ggcagcacgt
    16741 cctgccagct ccagcccacg gcatcgttgt tgccccgcag gatctcgtgc caggcgtcgt
    16801 agtccgaggg ccggccgcgc atgtagacct gcgcgttcac cgacgagccg ccgcccagca
    16861 ccctgccttg cgggatgtcg tgcacacggc cgcccagatg cgcctgcggc acggtctggt
    16921 ggtagcgcat gaacttcgag ccgttgatca tcttgaagat gccgggcggc atgtcgagca
    16981 gcggatggcg gttcgagagc cccgcctcca gcagcagcac cctggccccg gcccgggcca
    17041 gccgcccggc cacggtgcag cccgaggccc cgccgccgac gacgatgtga tcgaagcgct
    17101 cgctcatggc tgccccgcct ccgtccgctc gggcgccggc tcgaacgtca cccgcacata
    17161 ctcccaggcc gcgttcaaat gactggccag cgccgcctcg gccgcatccg ggtcgcgctc
    17221 gaggatcgcc tcgaggatcg tccggtggct ctggtagttc acccggttgc gctccggcga
    17281 gcgcagcatc cggtgccagt gcaccgccag ccaggcgaca taggcgcgat ggatggccgg
    17341 catgatgggg ttgcgcggaa tctcgtagag caccgcgtgg aaggcgacgt ccgtcgcgta
    17401 gaacctggcg gaatcgggaa tcgccgcctc gttggcggcc agcgcctcgc gcagcgcctg
    17461 gatgtcggca cgccgcgcat gcatcgccgc ctcgcgcacc agcgcccgct cgaggaagat
    17521 gcggctgtcg aagagcgcct tcaccccctc cgcctcgccg agcaggtggc cgacgatgcc
    17581 gcccaaggca ccgagagccg catcgaagcc cggcttgcgc acgatggggc gatagcgcgg
    17641 ccggttctcg atgaggccgc ggctggagag ggccgtgatc gcttcgcgga tgaccgtgcg
    17701 gctggcgccg aactccgcca tcagcacgcg ctccggcggc agcgccgtgc ggtccggcag
    17761 ctcgcccgag acgatgcgca gctcgagccg ctcgacaatc gcatcggccg cccgccggcc
    17821 gctcgagatc cgggcgtcca acgttatcct ccggccattt ttggtatgat caaaataccc
    17881 acgcccccat ccggcatttc agatgcagca ataatgccga cgaaaatcat gttgacgcaa
    17941 ggtagtccgg ccgtcaatct gttgtcgttt cgtcatacga aaatgagcgt ttgacgcgac
    18001 aagcgattgc caagagacgc acgatctgcg caatctccgg cccgggtgac cgggcgccgc
    18061 gcacgagagt caccgcctac cggatcagca aggaggaatc ccatgaaccg acgtttcctg
    18121 atggccaccg ccgccctcgc gggcctcggt gtcgccgcca tcgccgttcc cctggcggcc
    18181 caggacctgc cgccgctcgc gcagaaggac cgctacaagg tc
//
LOCUS       DAOTFI010000087        18133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3712_length_18133_cov_7.045304, whole genome shotgun sequence.
ACCESSION   DAOTFI010000087 DAOTFI010000000
VERSION     DAOTFI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18133
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3712_length_18133_cov_7.045304"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /gene="glpX"
                     /locus_tag="P5558_10570"
     CDS             complement(<1..969)
                     /gene="glpX"
                     /locus_tag="P5558_10570"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRY24795.1"
                     /translation="MTDYQSEDRNLALDSVRVTEAAALACARWMGRGDEKAADQAAVD
                     AMRHALNVLDIDGVVVIGEGERDEAPMLFIGEEVGTGRGPKLDIALDPLEGTTITAKG
                     GPNAIACLAMAARGAFLNAPDVYMQKIAIGGGLPQGVIGIEDSAGTNLGRLAEAKGVA
                     VEDLVVCILDRPRHEKLIAEVRAAGARIRLIGDGDVAGVIATSRPGSGIDIYMGSGGS
                     PEGVLAAAALRCIGGQLQGRLIFRNDEERARARKLGIEDLDRVYDVDDLAKGDVMFTA
                     TGVTDGTMLKGVRRVGDRLFTQSIVMRSKSGTVRLIEAEHNLPRKSA"
     gene            complement(1013..2311)
                     /locus_tag="P5558_10575"
     CDS             complement(1013..2311)
                     /locus_tag="P5558_10575"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRY24796.1"
                     /translation="MSGPTPLRVAIAGLGTVGAGTVRLLASDPARLALRAGRPIELRA
                     VSARDRGRDRGLALDGCRWVEDARELAFADDVDVVVELIGGADGIARELAAAALRRGK
                     AVVTANKAMLARHGAELAALADEAAVPLAFEAAVAGGIPIVKSLREGLSGDAVQRVFG
                     ILNGTCNYILTVMRETGRDFADVLEEAQQLGYAEADPSFDVDGIDAAHKLALLAAIAF
                     GGRPGFDAIHIEGIRHVSALDIEFAEELGYRIKLLGVARLTPSGLEQRLHPAMVRKDA
                     PIARVDGVFNAVGVLADPVGLVMHEGRGAGAGPTASAVVADLVDIARGIRLPTFGVPS
                     SALVDHPAAPMAAHEGCYYIRLMVLDQPGVLADVAAILRDQHVSIEALIQRARNPNQP
                     VPIVLTSHQTTEAAMTAALTAIEALPTVIEPPRMIRIEPN"
     gene            complement(2308..3540)
                     /locus_tag="P5558_10580"
     CDS             complement(2308..3540)
                     /locus_tag="P5558_10580"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRY24797.1"
                     /translation="MTVEPEFHRIRRLPPYVFAEVNAMKAAARARGRDIIDLGMGNPD
                     QPTPAHIVEKLVETVRNGRTHRYSTSRGITGLRRALARYYDRRFGVGLDPESEVCVTL
                     GSKEGLANLAQAITAPGDTILTPNPSYPIHPYGFIIAGAAIRHVELAPGRDLIRDLAR
                     SAEHSVPPPSAMILNFPSNPTALQVDLDFYREAVALAKARGMFILSDIAYAEIFFDRP
                     PPSILEVDGAFDVAVEFSSLSKTYSMPGWRIGFAAGNRRLIQALTRIKSYLDYGAFTP
                     IQVAATAALNGPQDCVEAARTLYRERRDVLVDGLAKAGWPVPKPTATMFCWAPLPESC
                     REMGSLAFAKLLLEEADVAVSPGIGFGEHGDGHVRMALVENKHRLRQAVRNIRAFLAR
                     RGVGAPVPVPEALLEAAS"
     gene            complement(3593..4450)
                     /locus_tag="P5558_10585"
     CDS             complement(3593..4450)
                     /locus_tag="P5558_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24798.1"
                     /translation="MTVRRRGWPGKAPRVRGFAILALGLGVLGGCGGLLDSVPPRPAA
                     IDDPAVAEAPFPRLAEVPARPRLGYTLEQRRAIAEGLVADRDNARHQGDELRRATGRP
                     ALPPAPPLPAQEPPRASPPPDPAADLAIAYVEDSLARDADDGSLGDFLDGLERLPPGI
                     DAAVVQAGVAAMPPLEGMPGAGGLPLHVVFPHGSAALGPDAEERLRAAGAALREATGP
                     VVVSAGGARAGPGELATAGLAMQRAQRVAAVLVAAGLDAGRISFDAGGEMGGDGDEVI
                     VYQPDGGSG"
     gene            4663..6468
                     /gene="phaC"
                     /locus_tag="P5558_10590"
     CDS             4663..6468
                     /gene="phaC"
                     /locus_tag="P5558_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRY24799.1"
                     /translation="MAKQAAAEAHANEAGANPEEIAQIMGEIAGRSRAIVEEFLQRQE
                     ISSGPGATPLNLGQTFLELTQRMMSSPHALMEAQFGLWQDYMRLWQTTTQRMLGHESD
                     PVIAPEKGDRRFRDAAWNDNILFDYIKQSYLLSSRYILEAAGEDRGMDPKEQQKVEFY
                     TRQFVDALAPSNFVMTNPEVLRATIDSRGENLLRGLKHMLEDLERGKGRLSIRMTDLD
                     AFEVGRNIATTPGKVVFQNELMQLLQYAPTTETVHKRPLLIVPPWINKFYILDLQPKN
                     SFIRFAVEQGFTVFVISWVNPDEALSHKTFESYMLEGPLAALDAIEQATGERDVTAIG
                     YCLGGTLMAATQSYLAARKDNRIKALTLFTALLDFTDPGELGVFIDEEQLAAIEDKMA
                     KRGYLDGAEMATTFNMLRANDLIWSFVVNNYLLGKEPFPFDLLYWNSDSTRMPAAMHN
                     FYLRRFYHENLLVEPGGIELGGVPIDLGRVRLPVYWVSTREDHIAPWKSTYQSTQIFK
                     GPKKFVLAGSGHIAGVINPPASGKYGYWLNDELPPAPEDWLAGAEHREGSWWEDWAAW
                     NASKAGARVPARVPGEGGLSALEDAPGSYVKVRAV"
     gene            complement(6495..7913)
                     /locus_tag="P5558_10595"
     CDS             complement(6495..7913)
                     /locus_tag="P5558_10595"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HRY24800.1"
                     /translation="MRSGALADLVPVILAGGSGTRLWPVSRAAFPKHLVELTGESSLL
                     QATASRLLALASPGRFLTVAAAGQAILVRRQLALVDPALATHLLLEPAPRNTAAAVAL
                     AALYARDAWGGEALLWICPSDHLIADPAPLRAAVVAGAAAARAGHLVTFGIEPTRPET
                     GFGYIAQGDPLDLPGVRGVRRFVEKPNLSVAEAMLAAGDHLWNSGMFLMRASTILEEL
                     EAHAPDILAATRDAMSAAGTVPHTIDPERFGRVPSLPIDKAVMERSQRVAVVPCDPGW
                     SDVGSWQAIWEIMPKDPAGNALRGDVVVAGGRGNLIRAEHRLVAVAGVDDLAVIETAD
                     AVMVAPKADADAVKALVARLVEAGRPEALVHAREVRPWGAFTVLAAGPGYMVREVEID
                     PGAALDRQRHAGRDEHWTVVGGELALESADGVARHAVGAAVTAARGQTHRLSNPAGET
                     LRLIEVRIGSDLDDVPLAQLAG"
     gene            8107..9153
                     /locus_tag="P5558_10600"
     CDS             8107..9153
                     /locus_tag="P5558_10600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY24801.1"
                     /translation="MRDAEERQATSRFAEQALATMRSYAIPPTPENFAVWYLHHAGRL
                     PGLSRAIERLEERRTGVTADETAALHRSFCAAADHAGEVQAAGERVGELVDEVSRRLD
                     VASAHTRDYGERLTTIGNGLSTTVDPGQLVAMVRELERQTELMNERVRTLQRELNQNS
                     DEIENLKGDLASAREAASTDPLTGIGNRKLFDEAFAWETQQAATSGEPLALLVADIDH
                     FKAFNDAHGHQIGDIVLKLVADRLKRSVKGRDTVARYGGEEFAVLLPRTALADAAAVA
                     EQLRGEIAKSRLVMRNRGQELGKVTLSIGVALYRSGESTDTCFERADQALYAAKHAGR
                     NRVMVEGVLETAAA"
     gene            9525..11198
                     /locus_tag="P5558_10605"
     CDS             9525..11198
                     /locus_tag="P5558_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRY24802.1"
                     /translation="MTPTGFRTGAHPPASAYERDLDRTAANHAPLTPLTFLERTASVY
                     PEHVAVVHGPTRHTYAELNDRCRRLAAGLAGLGVAPGDTVAIMAPNIPPLLEAHYGVP
                     MAGAVLNALNYRLDAASIAFILEHGEATVLLADSEFAPVLREALAQTTRSIQVVDIQD
                     DAFRGQHERLGSLTYEELLARADPAFVPYLPADEWQAIALNYTSGTTGNPKGVVYHHR
                     GAHLNAVGNVLVWGMGHHPVYLWTLPMFHCNGWCFPWTIVLQAGTHVCLRRVEAGTIF
                     AALAEERVTHLCGAPVIMGMLVNAPAAERRPFAQTVRMMTAASAPPAAVLEKVEALGI
                     EVTHVYGLTEVYGPATVCAFHEAWRELPAAERASRLARQGVRYPVLEGLEVMDPATME
                     PVPADGATIGEIMMRGHDVMKGYLKNPEANATAFAGGWFHTGDLAVSHPDGYVEIRDR
                     SKDIIISGGENISSIEVEGALYRHPDVLEAAVVARPDETWGESPCAFVTLRDGSTVTA
                     ADIIAHCRRELAHFKAPRTIVFGPLPKTSTGKVQKFQLRERARSLDTQD"
     gene            11213..12391
                     /locus_tag="P5558_10610"
     CDS             11213..12391
                     /locus_tag="P5558_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY24803.1"
                     /translation="MDAELAAARALPRDPDRATLVGRAWLPGEPGGPSVVTVRGGEVH
                     DLTRLAPTMADLINAPDPVALARDERAVRVGPLTALLEASHADRVSERAAHLLAPTDL
                     SAHKAAGVTFVKSMLERVVEEATRGDPDAAAAARARIEGLIGGNLTAIVPGSEAAAKL
                     KAHLIEAGAWSPYLEVGVGVDAEIFTKAQPMSAVGTGAEIGIHPRSQWNNPEPEVVLV
                     IAADGRIVGATLGNDVNLRDFEGRSALLLGKAKDNNAACAIGPFIRLVDDRFTVDTIR
                     GLVVALEVRGTDGFVLQGSSSMREISRDIENLVGHLMGGTHQYPDGAVLFCGTMFAPV
                     EDRDVPGHGFTHKLGDVVCIATPELGCLVNRINHADKAPPWTFGARQLLHNLHARGLL
                     "
     gene            complement(12504..12710)
                     /locus_tag="P5558_10615"
     CDS             complement(12504..12710)
                     /locus_tag="P5558_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRY24804.1"
                     /translation="MSLHEHVDSLRAKHARLEEQIDEEQHRPLPDSTVLVKLKREKLR
                     LKEAIERLQHDGEDQSAPPGMAMN"
     gene            complement(12866..13429)
                     /locus_tag="P5558_10620"
     CDS             complement(12866..13429)
                     /locus_tag="P5558_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HRY24805.1"
                     /translation="MTGATAWPDCAFWDFSLALYARPGIEAICLMLQDDFGLDVNLVL
                     LAAWTASRGTKLAPDLAARLRSLGEGYQATVMQPCRAARRGLRGWPEMTALGDLAAAP
                     ERRLRALELDLERLEQLRLAALVESRHPAPEASGPDLFRANLEALYPGRALPEPVLET
                     FAAGVASLRPKRRAAPFNPICPRDSKA"
     gene            complement(13426..14376)
                     /gene="glk"
                     /locus_tag="P5558_10625"
     CDS             complement(13426..14376)
                     /gene="glk"
                     /locus_tag="P5558_10625"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRY24806.1"
                     /translation="MTRLIADIGGTNARFALTDAVGMPSRERRLAVAAHAGPGEAAKA
                     YLAALFEPPGSIDEAVIAVATPVDAETIRFTNSPWTFTRDQLQAELGIARLVVINDFA
                     AQAAAIPYLGPADSVAIGGGEADPDWPIAVIGPGTGLGAAALVPGPDGPIVVASEAGH
                     VSFAPQDDQEIELLRHLRARHGHVSNERLLSGPGLLALAESLAALTSSELAATSPETV
                     TRAARDGSCPVSVAALQRFSAMLGAAAGDLALTFCAKGGVYVSGGLCPKLGPLFDRAG
                     FRRAFEAKGRLGAFLARVPVRLVTTERIGLLGAAVHRLTP"
     gene            14509..14718
                     /locus_tag="P5558_10630"
     CDS             14509..14718
                     /locus_tag="P5558_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HRY24807.1"
                     /translation="MRKPGDHNEIDTRLASLRAAHRALDCRIGQLTDESFVDQVALQR
                     LKKQKLILKDRISQLESDRYPDIIA"
     gene            complement(14708..15772)
                     /locus_tag="P5558_10635"
     CDS             complement(14708..15772)
                     /locus_tag="P5558_10635"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRY24808.1"
                     /translation="MSLAPGATIGVLGGGQLGRMTALAAARLGCRAAVLAPEAEAPAA
                     DVAWRHWQAGYDDSGALAEFLAVVDVVTLEWENVPVSALAAIAAQKPMRPGAEVLRIT
                     QDRLLEKSFVAGLDVPVAPFARVDDVDALMAGLDQLGPRAVLKTRRFGYDGKGQEVLG
                     PESRSADAFTRLGGEALILEAFVPFERELSVIVARRPNGEMRCFPPVENRHQAQILVE
                     TIAPAELPGSVARSAIDIAERIAAALRLEGLLAVEMFLEPGGRLLVNELAPRPHNSGH
                     WTIDGCTVSQFEQQVRAILDLPLGDTTPLAPCVMQNLLGDAIDAWPAILAEPGACLHL
                     YGKREARPGRKMGHVTRFRR"
     gene            complement(15769..16293)
                     /gene="purE"
                     /locus_tag="P5558_10640"
     CDS             complement(15769..16293)
                     /gene="purE"
                     /locus_tag="P5558_10640"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRY24809.1"
                     /translation="MIGAVPVAVIMGSQSDWPTMRHAVAVLRELDIACDTRIVSAHRT
                     PERLVAFASGAAGEGFRVVIAGAGGAAHLPGMVAAMTDLPVLGVPVESRALSGLDSLL
                     SIVQMPAGVPVGTLAIGAAGATNAGLLAAAMLATTDSGLAQRLRAYRDAQTAAVAERP
                     SDGDPADVQPAGPA"
     gene            complement(16309..16560)
                     /locus_tag="P5558_10645"
     CDS             complement(16309..16560)
                     /locus_tag="P5558_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HRY24810.1"
                     /translation="MLYLTRRIGEAVIINNAIEVRVVELKGKTVKLGFTFPPSATVLR
                     EEIYLQIKRENEAAAAAVGALRGGDRQPGTVADADPDGD"
     gene            16675..17091
                     /locus_tag="P5558_10650"
     CDS             16675..17091
                     /locus_tag="P5558_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRY24811.1"
                     /translation="MAAPLPALFQLLSARAGFLQARHAVLAQNVANADTPRYQPQDLV
                     EPAFEALLRGGPGSTPSVAVARTRSAHLAGASPGAASPRQRPIEAFETAPAGNAVIIE
                     EQIRLMGQADRGYQLATNLYGKYVGMMRTALGAGGA"
     gene            17133..17540
                     /gene="flgC"
                     /locus_tag="P5558_10655"
     CDS             17133..17540
                     /gene="flgC"
                     /locus_tag="P5558_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRY24812.1"
                     /translation="MDLETSMRIAAAGMQAQSARLRVTAENLANAQSTAESPGGDPYQ
                     RKTISFANALDRATGARLVEVRRYGTDPAAFERRFEPSHPAADASGYVLYPNVNPLIE
                     LMDMREAQRSFEANLTALQQARGMLQRTLDLLR"
     gene            17599..17910
                     /locus_tag="P5558_10660"
     CDS             17599..17910
                     /locus_tag="P5558_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRY24813.1"
                     /translation="MSIGAANQALAAYRAAASEIAGGKQATTAAGIGSFDGLLQAELR
                     STLATLEASEQASIGAVSGRVGIQQVVEAITAAELSLQKVTAVRDRVISAYQEIIRMP
                     I"
     gene            17946..>18133
                     /locus_tag="P5558_10665"
     CDS             17946..>18133
                     /locus_tag="P5558_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HRY24814.1"
                     /translation="MLTDLMVQDVARQAIWVMLKVGAPMMLVALVVGLCVSLLQALTQ
                     IQEMTLTFVPKILAMFMTL"
BASE COUNT         2763 a         6443 c         6394 g         2533 t
ORIGIN      
        1 ggcgctcttg cgcggcaggt tgtgctcggc ctcgatcagc cggacggtgc cgctcttcga
       61 gcgcatcacg atcgattgcg tgaagagccg gtcgccgacg cgccgcacac ccttgagcat
      121 ggtgccatcg gtgacgccgg tggcggtgaa catgacgtcg cccttggcga ggtcgtcgac
      181 gtcgtagacg cggtcgagat cctcgatgcc gagcttcctc gcgcgcgccc gctcctcgtc
      241 gttgcggaag atcagccggc cctggagctg gccgccgatg cagcgcagcg ctgccgcggc
      301 cagcacacct tccggcgagc cgccggagcc catgtagatg tcgatgccgc tgcccggccg
      361 cgaggtcgcg atgacgccgg cgacgtcgcc gtcgccgatc agccggatgc gagcaccggc
      421 ggcgcgcacc tcggcgatca gcttctcgtg gcgcggccgg tcgaggatgc agacgacgag
      481 atcctccacg gcaacgccct tggcctcggc gagacgcccg agattggttc cggcgctgtc
      541 ctcgatgccg ataacgccct gcggcaggcc accgccgatg gcgatcttct gcatgtagac
      601 gtcgggggcg ttgaggaagg cgccccgcgc cgccatggcg aggcaggcga tggcgttggg
      661 gccgcccttg gcggtgatcg tggtgccctc cagcggatcg agggcgatat cgagcttggg
      721 ccctcgcccc gtgccgacct cctcgccgat gaagagcatc ggcgcctcgt cgcgctcgcc
      781 ctcgccgatc accacgacgc cgtcgatatc gagcacgttg agcgcgtgcc gcatcgcgtc
      841 gaccgctgcc tggtccgccg ccttctcgtc gccgcggccc atccagcggg cgcaggcgag
      901 cgcggccgcc tcggtgaccc ggacggaatc cagtgccaga ttgcggtctt cgctctggta
      961 gtcggtcatc tcgttcctcg ttgcgaacac ccgccgcagc ccgcggccgg gctcaattcg
     1021 gctcgatacg gatcattcgg ggcggctcga tgacagtcgg caacgcctcg atcgccgtca
     1081 gcgcggcggt catggcggcc tccgtggtct ggtggctggt caggacgatc ggcaccggct
     1141 ggttggggtt gcgggcacgc tggatcagcg cctcgatcga cacgtgctgg tcgcgcagga
     1201 tggccgccac atcggcgagc acgcccggct ggtcgagcac catcagccgg atgtagtagc
     1261 agccctcgtg cgccgccatg ggtgctgccg ggtggtcgac gagggcgctg ctcggcacgc
     1321 cgaaggtcgg caggcggatg ccgcgggcga tgtcgacgag atcggccacc accgccgagg
     1381 cggttgggcc ggcgccggcc ccccggcctt cgtgcatgac gaggccgacc ggatcggcga
     1441 gcacgccgac ggcgttgaag acgccatcga cccgggcgat cggcgcgtcc ttcctgacca
     1501 tcgccggatg gagccgctgc tcgaggccgg acggcgtcag ccgcgccacg cccaggagct
     1561 tgatccggta gccgagctcc tcggcgaact cgatgtcgag cgcgctgacg tggcggatgc
     1621 cctcgatgtg gatggcatcg aagcccggcc tgccgccgaa ggcgatggcg gcgagcaaag
     1681 cgagcttgtg ggcggcgtcg atgccgtcga cgtcgaagct cggatcggcc tcggcgtagc
     1741 cgagctgctg cgcttcctcg agcacgtcgg cgaagtcgcg gccggtctcg cgcatcaccg
     1801 tcaggatgta gttgcaggtg ccgttgagaa tgccgaagac ccgctgcacc gcatcgccgc
     1861 tgagcccctc gcgcaggctc ttgacgatcg gaatgccgcc cgcgaccgcc gcctcgaagg
     1921 ccagcggcac cgctgcctcg tcggccagcg ccgcaagctc ggcgccgtgc cgggcgagca
     1981 ttgccttgtt ggcggtcacc acggccttgc cccgccgcag cgcggcggca gcgagctcgc
     2041 gcgcgatgcc gtcggcacca ccgatcagct cgaccaccac gtcgacatcg tcggcgaagg
     2101 ccagctcgcg ggcgtcctcg acccagcggc agccatcgag cgccagaccc cggtcgcgcc
     2161 cgcggtcgcg cgccgagacc gcccgcagct cgatcggccg accggcgcgc agcgccagcc
     2221 gcgccggatc gctcgccagg agcctgacgg tgccggcacc caccgtgccg aggccggcaa
     2281 tggcgacgcg cagcggtgtg ggcccgctca agacgcggcc tccaggagcg cttcgggcac
     2341 gggcaccggc gcgcctaccc cgcgtctggc caggaatgcc cggatattgc gcacggcctg
     2401 gcgcagccgg tgcttgttct cgaccagcgc catgcggaca tggccgtcgc catgctcgcc
     2461 gaagccgatg ccgggcgaga ccgcgacatc cgcctcctcg agcagcagct tggcgaaggc
     2521 gagcgagccc atctcgcggc agctttccgg gagcggcgcc cagcagaaca tggtggcggt
     2581 cggcttcggc accggccagc cggccttggc cagcccgtcg accagcacgt cgcggcgctc
     2641 gcggtagagg gtgcgggccg cctcgacgca gtcctgcggc ccgttcagtg cggccgtcgc
     2701 cgccacctgg atcggggtga aggcgccgta gtcgagatag gacttgatgc gggtgagcgc
     2761 ctggatcagg cggcggttgc cggcggcgaa gccgatccgc cagcccggca tggagtaggt
     2821 cttggagagg ctcgagaact cgacggcgac gtcgaaggcg ccgtcgacct cgaggatcga
     2881 cggtggtggc cggtcgaaga agatctcggc ataggcgatg tccgagagga tgaacatgcc
     2941 gcgtgccttg gccagcgcca cggcctcccg gtagaagtcg agatcgacct gcagcgccgt
     3001 cgggttggac gggaagttga ggatcatggc cgagggcggc ggcaccgagt gctcggccga
     3061 gcgggcgagg tcgcggatca ggtcgcgccc gggcgccagc tcgacatggc ggatggcggc
     3121 gccggcgatg atgaagccat aagggtggat cgggtagctc gggttggggg tgaggatggt
     3181 gtcgccgggt gcggtgatcg cctgggcgag gttggcgagc ccttccttgg acccgagcgt
     3241 gacgcagacc tcgctctccg ggtcgagccc cacgccgaag cggcggtcgt agtagcgggc
     3301 gagcgcccgg cgcaggccgg tgatgccccg gctggtcgag tagcggtggg tgcggccgtt
     3361 ccgcaccgtc tcgaccagct tctcgacgat gtgcgccggc gtcggctggt cggggttgcc
     3421 catgccgaga tcgatgatgt cgcggccgcg cgcgcgggcc gccgccttca tcgcgttgac
     3481 ctcggcgaag acgtagggcg gcaggcggcg gatgcggtgg aattcgggtt cgacggtcat
     3541 cgatcgggct ctgggctcag gaacggggtc atccggcggg cggccggaca cgtcacccgg
     3601 agccaccgtc gggctggtat acgatcacct cgtcgccgtc gccacccatc tcgccgcccg
     3661 cgtcgaagga gatgcgcccg gcatcgaggc cggctgcgac cagaacggcg gcgacccgtt
     3721 gcgcgcgctg catggccagg ccagccgtcg ccagctcccc cggcccggcc cgagcgccgc
     3781 cggcgctgac gacgaccggc ccggtcgcct cgcgcagcgc cgcgcccgcc gcccgcagcc
     3841 gctcctcggc gtccggcccg agggcagccg agccgtgcgg gaagacgacg tgcagcggca
     3901 ggccgcccgc gcccggcatg ccctccagcg gtggcattgc ggcgaccccg gcctggacca
     3961 ccgccgcatc gatccccggc ggcaggcgct ccagcccgtc gaggaaatca ccgagcgacc
     4021 cgtcatcggc gtcccgggcc agcgagtcct cgacataggc gatggccagg tccgccgccg
     4081 gatcgggtgg cggcgacgcc ctgggcggct cctgcgccgg gagcggcggg gccggcggca
     4141 aggctgggcg accggtggcc cggcgcagct catcgccctg gtgacgcgcg ttgtcgcggt
     4201 cggcgacgag cccctcggcg atcgcccggc gctgctccag ggtgtagccg aggcgcggcc
     4261 gcgcgggcac ctccgccagt cgcggaaagg gcgcctcggc gaccgccggg tcgtcgatcg
     4321 cggccggccg gggcggcacg gaatcgagca gcccgccgca cccgccgagc acgccgagcc
     4381 ccagcgccag gatcgcgaag ccacggaccc tcggggcctt gccgggccaa cctcgccttc
     4441 gtaccgtcat ccgagctcca ctggctccat ccccggaccc gccgggcccg tcccgatccg
     4501 gcgtcatttg tcgcgaccgc cggagttggc gcaaggccct gcttcggcta gaccgaaccg
     4561 cgctggactg gcgagcgcgg gcggcatgtg gtcggtggat gcatcccttc ggcctcgcac
     4621 gggcgccgca ccggcaggcg aagcaaggta gggtaagatc gaatggcgaa gcaagcagca
     4681 gccgaggccc acgcgaacga ggccggcgcc aacccggagg agatcgccca gatcatgggc
     4741 gagatcgccg ggcgcagccg cgccatcgtc gaggagttcc tgcagcgcca ggagatctcg
     4801 agcgggccgg gcgccacgcc gctcaatctc gggcagacct ttctcgagct gacccagcgg
     4861 atgatgtcga gcccgcatgc gctcatggag gcgcagttcg ggctctggca ggactacatg
     4921 cggctctggc agacgacgac gcagcggatg ctcggccacg agagcgaccc ggtgatcgca
     4981 cccgagaagg gtgaccggcg gtttcgcgat gccgcctgga acgacaacat cctcttcgac
     5041 tacatcaagc agtcctacct cctgagctcg cgctacatcc tcgaggccgc cggcgaggat
     5101 cgcggcatgg acccgaagga gcagcagaag gtcgagttct acacgcggca gttcgtcgat
     5161 gccctggccc cgtcgaactt cgtcatgacc aatccggagg tgctgcgcgc gaccatcgac
     5221 agccgcggcg agaacctgct gcgcggcctc aagcacatgc tcgaggatct cgagcgcggc
     5281 aagggccgcc tctcgatccg gatgaccgat ctcgatgcct tcgaggtcgg ccggaacatc
     5341 gccacgacgc ccggcaaggt ggtcttccag aacgagctga tgcagctcct gcaatacgcg
     5401 ccgacgacgg agacggtgca caagcggccg ctgctgatcg tgccgccctg gatcaacaag
     5461 ttctacatcc tcgacctgca gcccaagaac agcttcatcc gcttcgcggt cgagcagggc
     5521 ttcaccgtgt tcgtcatctc ctgggtcaac ccggacgagg cgctcagcca caagaccttc
     5581 gagagctaca tgctggaggg gcccctggcg gcgctcgacg cgatcgagca ggccaccggc
     5641 gagcgcgacg tcaccgccat cggctactgc ctcggcggca cgctgatggc ggcgacgcag
     5701 tcctatctgg cggccaggaa ggacaaccgg atcaaggcgc tgaccctgtt cacggcactg
     5761 ctcgacttca ccgacccggg cgagctcggc gtcttcatcg acgaggagca gctcgcggcc
     5821 atcgaggaca agatggccaa gcgcggctat ctcgacggtg ccgagatggc gacgacgttc
     5881 aacatgctgc gcgccaacga cctgatctgg tcgttcgtcg tcaacaacta cctgctcggc
     5941 aaggagccgt tcccgttcga cctgctctac tggaacagcg acagcacgcg catgccggcg
     6001 gccatgcaca atttctatct ccgccgcttc tatcacgaga acctgctggt cgagccgggc
     6061 ggcatcgagc tcggcggcgt gccgatcgac ctcgggcggg tgcgcctgcc ggtctactgg
     6121 gtctcgaccc gcgaggacca catcgccccc tggaagagca cctaccagtc gacgcagatc
     6181 ttcaagggtc cgaagaagtt cgtcttggcc ggctccggcc acatcgccgg cgtcatcaac
     6241 ccgccggctt cgggcaagta cggctactgg ctgaacgacg agctgccgcc ggcgccggag
     6301 gactggctgg ccggggcgga gcaccgcgag ggctcgtggt gggaggactg ggcggcctgg
     6361 aacgcgtcca aggccggtgc ccgggtgccg gcgcgcgtgc ccggcgaggg cgggctctcg
     6421 gcgctcgagg acgcacccgg cagctatgtg aaggtgcgcg ccgtctgagc cggcacggtc
     6481 cgggccagca ccgctcagcc ggcgagctgg gccaggggga cgtcgtcgag atcgctgccg
     6541 atgcgcacct cgatcagccg gagcgtttcg ccggcggggt tgctcaggcg atgcgtctgg
     6601 ccgcgggcgg cggtgacggc ggcgcccacg gcgtgccggg caacgccatc ggcggactcc
     6661 agcgcgagct cgccccccac cacggtccag tgctcgtcgc gcccggcgtg gcgctggcgg
     6721 tcgagcgcag cacccgggtc gatctccacc tcgcgaacca tgtagcccgg cccggccgcg
     6781 aggacggtga aggcgcccca gggacgcacc tcgcgcgcat ggaccagggc ctcgggccgg
     6841 ccggcctcga ccagccgggc gaccagcgcc ttgaccgcat cggcatcggc cttgggtgcc
     6901 accatgacgg catcggcggt ctcgatcacc gcgaggtcgt cgacgccggc gaccgccacc
     6961 aggcgatgct cggcccggat cagattgccg cgaccgccgg ccacgaccac gtcgccgcgc
     7021 aaggcgttgc cagccggatc cttgggcatg atctcccaga tcgcctgcca cgacccgaca
     7081 tccgaccagc cggggtcgca gggcacgacg gcgacgcgct gcgagcgctc catcaccgcc
     7141 ttgtcgatcg gcagggacgg cacccggccg aaacgctccg gatcgatcgt gtggggaacc
     7201 gtgccggcag cgctcatggc gtcccgggtg gcggccagga tgtcgggcgc atgcgcctcc
     7261 agctcctcca ggatggtgga ggctcgcatc aggaacatgc cgctgttcca gaggtggtcg
     7321 ccggccgcca gcatcgcctc ggcgaccgag aggttcggct tctcgacaaa gcgtcggacg
     7381 ccacgcacgc ccggcagatc cagcggatcg ccctgggcga tgtagccgaa cccggtctcc
     7441 ggccgcgtcg gctcgatgcc gaaggtgacg agatggccgg cccgggccgc ggccgcgccg
     7501 gcgaccacgg ccgccctgag cggcgccggg tcggcaatca ggtgatccga cgggcagatc
     7561 cagagaagcg cctcgccgcc ccaggcatca cgggcataga gcgctgccag cgccaccgcc
     7621 gccgcggtgt tgcgcggcgc cggctcgagc aggagatggg tggccagagc gggatcgacc
     7681 agggcgagct gacggcgaac gaggatggcc tgccccgccg ccgcgacggt caggaaccgg
     7741 ccgggcgatg ccagcgccag caaacgggat gccgtcgcct ggagcagcga gctctcgccg
     7801 gtcagctcga ccagatgctt cggaaaggcg gcacggctca ccggccagag gcgggttccc
     7861 gagccgccgg cgaggatgac cgggacgaga tccgcaaggg cgcctgatcg cacgaagatc
     7921 tcccgtctgc gccgccgcga ccgcgcggcg ttgaagcgac ggaccttcta ccacgcgaca
     7981 cggcctgcga aacccgtcta tcggccgcac cggccgtgcc ctgagcacgc ccttgttaac
     8041 cctttcgaca tttcggcgtg gcagactcgg gcaagatcga gttcgagagc gggcaggagc
     8101 cttcagatgc gggatgccga ggagcggcag gccaccagtc gttttgccga gcaggcgctg
     8161 gcgacgatgc ggtcgtacgc cattccgccg acaccggaga atttcgccgt gtggtacctg
     8221 catcatgccg gccgcctgcc gggcctgagc cgggcgatcg agcggctcga ggagcggcgc
     8281 accggcgtca cggcggacga gacggcagcg ctccaccggt cgttctgcgc cgcggccgat
     8341 catgccggcg aggtccaggc ggccggcgaa cgggtcggcg agctggtcga cgaggtcagt
     8401 cgccggctcg atgtcgcgag cgcgcacacg cgcgactacg gcgagcggct gaccacgatc
     8461 ggcaacggcc tgagcacgac cgtcgacccg gggcagctcg tcgccatggt gcgcgagctg
     8521 gagcggcaga ccgagctgat gaacgagcgt gtgcgcaccc tgcagcgcga gctcaaccag
     8581 aacagcgacg agatcgagaa cctgaagggt gacctcgcca gcgcgcgcga ggcggcgagc
     8641 accgatcccc tgacgggcat cggcaatcgc aagctgttcg acgaggcctt cgcctgggag
     8701 acgcagcagg ccgcgacgtc gggcgagccc ctggccttgc tggtcgccga catcgaccac
     8761 ttcaaggcgt tcaacgacgc ccacggccac cagatcggcg acatcgtgct gaagctggtg
     8821 gccgatcggc tgaagcgctc ggtcaagggc cgcgacaccg tcgcccgcta tggcggcgag
     8881 gagttcgccg tcctcctgcc gcgcaccgcg ctcgccgacg ccgccgcggt cgcggagcag
     8941 ctccgcggcg agatcgcgaa gagccggctg gtgatgcgca accgcggaca ggagctcggc
     9001 aaggtgacgc tgtcgatcgg tgtggcgctc taccggtcgg gcgagtccac cgacacctgc
     9061 ttcgagcgcg ccgaccaggc cctctatgcc gcgaagcacg ccggtcgcaa ccgggtcatg
     9121 gtcgagggcg tgctcgagac tgcggcggcc tgaggggtcg cgtccgggcc gcgctgcccg
     9181 ctgctcgccg gtgccgatcg accgggcgtg cttccggctg cagccctttc cgctgtcggt
     9241 ggaacatgca gaacgatgcc aggcgatcat gcctgatgac ggatcgcggc cggcggccct
     9301 cgccgtcgcg cccgccgctg gcctgtggaa cgtcgaggcc agccgttcgg tcgtcgcgcg
     9361 acgggcatct cccggcaccg gtcgagcgcg atccgcggtc tgccgcttga cgggcgtcgc
     9421 gtcgcatagc agacccgtct gccagcaacc cctgccgtcg ctgccggcgg ttgcggtggc
     9481 gaggcggcgg cggcaagacc gtcgtcgcaa tcgggaggcc gaacttgacg ccaacgggct
     9541 ttcgcaccgg tgcgcatccg ccggcttcgg cttacgagcg ggatctcgac cgcacggccg
     9601 ccaaccatgc gccgttgacg cccctgactt tcctcgagcg gaccgccagc gtctatcccg
     9661 agcatgtcgc cgtggtgcac ggcccgaccc gccacaccta tgccgagctg aacgatcgct
     9721 gccgccggct cgccgccggg ctcgccgggc tcggcgtggc gccgggcgat accgtggcga
     9781 tcatggcgcc gaacattccg cccttgctcg aggcgcatta cggcgtgccg atggcgggag
     9841 cggtgctcaa cgccctgaac taccggctcg atgccgcatc gatcgccttc attctggagc
     9901 acggcgaggc caccgtcctg ctcgccgaca gcgagttcgc gccggtcctg cgcgaggcgc
     9961 tggcgcagac gacccggtcg atccaggtcg tcgacatcca ggatgacgcg tttcggggcc
    10021 agcacgagcg cctcggctcg ctgacctacg aggagctgct ggcccgcgcg gatccggcct
    10081 tcgtgcccta tctgccggcg gacgaatggc aggcgatcgc gctcaactac acctcgggca
    10141 cgaccggcaa cccgaagggc gtcgtctacc atcaccgggg tgcccatctg aacgcggtcg
    10201 gcaatgtcct ggtctggggc atgggccatc atccggtcta tctctggacc ctgccgatgt
    10261 tccactgcaa cggctggtgc ttcccctgga ccatcgtcct gcaggccggg acgcatgtct
    10321 gcctgcgtcg ggtcgaggcc ggcaccatct tcgcggcgct ggcggaggag cgggtcacgc
    10381 atctctgcgg tgcgccggtc atcatgggca tgctggtcaa tgcgccggcg gccgagcgcc
    10441 ggccgttcgc gcaaaccgtg cggatgatga ccgcggcgag cgcgccgccg gccgcggtgc
    10501 tggagaaggt cgaagcactc ggcatcgagg tgacccatgt ctacggcctg acggaggtct
    10561 acgggccggc cacggtctgc gccttccacg aagcctggcg cgagctcccg gcggccgagc
    10621 gggcatcgcg cctcgcccgc cagggtgtgc gctacccggt gctcgagggc ctcgaggtca
    10681 tggacccggc gacgatggag ccggtgccgg ccgatggggc gacgatcggc gagatcatga
    10741 tgcgtggcca cgatgtcatg aagggctacc tcaagaatcc cgaggcgaac gccaccgcct
    10801 tcgccggcgg ctggttccat accggcgatc tcgccgtcag ccatcccgac ggctatgtgg
    10861 agatcaggga ccggtcgaag gacatcatca tctcgggcgg cgagaacatc tcgtcgatcg
    10921 aggtcgaggg cgctctttat cgtcatcccg atgtgctgga ggcagccgtg gtcgcccgcc
    10981 cggacgaaac atggggcgaa agcccctgcg ccttcgtcac gctgcgcgat ggctcgacag
    11041 tgacggcagc cgacatcatc gcgcattgcc gccgcgagct cgcgcatttc aaggcgccaa
    11101 ggaccattgt cttcgggccg ctgcccaaga cctcgaccgg caaggtgcag aagttccaat
    11161 tgcgcgagcg ggctcgctcg ctcgacacac aggattgagg aagggaatcg ccatggacgc
    11221 cgagctcgca gcagcacggg cgctgccccg ggatcccgac cgggccacgc tggtcggccg
    11281 cgcctggctg ccgggcgagc ccggcgggcc gagtgtcgtc acggtgcggg gcggcgaggt
    11341 ccacgacctg acgcggctgg ccccgaccat ggcggatctg atcaatgctc ccgatcccgt
    11401 ggccctggca cgggacgaac gcgccgttcg cgtcggcccg ctgacggcat tgctcgaggc
    11461 cagccacgcc gaccgcgttt cggagcgggc ggcgcatctc ctcgcaccga ccgacctctc
    11521 cgcgcacaag gcggccgggg tcaccttcgt caagagcatg ctcgagcgcg tggtggagga
    11581 ggcgacgcgc ggcgatcccg acgccgcggc cgcggcgcgc gcccggatcg agggcctgat
    11641 cggcggcaac ctcacggcca tcgtgccagg ctccgaggcg gcggcgaagc tgaaggcgca
    11701 cctcatcgag gccggcgcct ggtcgcccta tctcgaggtc ggggtcggcg tggacgcgga
    11761 gatcttcacc aaggcgcaac cgatgagcgc cgtcggcacc ggggccgaga tcggcatcca
    11821 cccgcgctcc caatggaaca atcccgagcc cgaggtggtg ctggtgatcg ccgccgatgg
    11881 gcgcatcgtc ggcgcgacgc tcggcaacga tgtcaacctg cgcgatttcg aggggcgcag
    11941 cgccctcctg ctcggcaagg ccaaggacaa caacgccgcc tgcgccatcg gcccgttcat
    12001 ccgtctcgtc gacgaccgct tcacggtcga cacgatccgc ggcctcgtcg tcgcgctcga
    12061 ggtcaggggc accgacggct tcgtcctcca gggctcgagc tcgatgcgcg agatcagccg
    12121 cgacatcgag aacctcgtcg ggcacctgat gggcggcacc catcaatacc ccgacggggc
    12181 ggtgctcttc tgcggcacga tgttcgcgcc ggtcgaggac cgcgacgtgc cggggcacgg
    12241 cttcacccac aagctcggcg acgtcgtctg catcgcgacc ccggagctcg gctgcctggt
    12301 caaccggatc aaccacgccg acaaggcgcc accctggacc tttggtgccc gccagctcct
    12361 gcacaacctg cacgcgcgcg gcctgctctg agcacagcag caacgaaaag gccggcgccc
    12421 cgtcgggcac cggccttccc cttccttcgg cgcacgcccc cgcccggctc gtccgccgac
    12481 agcgggggcc gagtcgcgta gcgtcagttc atcgccattc cgggtggcgc ggattggtcc
    12541 tcgccgtcat gctgcagcct ctcgatcgct tccttcaagc gcagcttctc ccgcttgagc
    12601 ttcaccagca ccgtgctgtc ggggagcggc cggtgctgct cctcgtcgat ctgctcctcc
    12661 agacgggcgt gcttggcgcg aaggctgtcg acgtgctcat gcagtgacat ggtcgggaaa
    12721 acccccctct tctggcctgt gggagcacca ctctagagaa acctctcggt ggccagcaag
    12781 agaaatccgt tgcgtcgttc cgccagacgt gtatgttgcg cggttttcca tcgctggcgg
    12841 ttgaattcaa ttgttggttg atcgatcaag cttttgaatc tctaggacaa atcgggttga
    12901 aaggggcggc gcggcgcttc ggccggagcg aggcgacccc cgctgcgaac gtctcgagca
    12961 ctggctcggg cagcgctcgg ccgggataga gcgcctcgag gttcgccctg aacaggtcgg
    13021 ggccgctcgc ttcgggcgcg ggatggcggc tctcgaccag ggcggcgagg cgaagctgct
    13081 ccagccgctc gagatccagc tcgagcgcgc gcagccggcg ctcgggggct gcggcgaggt
    13141 cgcccagcgc cgtcatctcc ggccagcccc gcaggccgcg ccgggccgcg cgacaaggct
    13201 gcatgaccgt cgcctgatag ccctcgccga gcgaccgcag tcgagccgcg agatcaggcg
    13261 cgagcttggt gccccgggaa gcggtccagg cggcgagcag aacgaggttg acgtccagac
    13321 cgaaatcgtc ctgcagcatc aggcagatgg cctcgatgcc cggtcgcgcg tagagcgcca
    13381 gggagaaatc ccagaatgcg cagtcgggcc aggcagtggc accggtcacg gggtgagccg
    13441 atgcacggca gcaccgagca ggccgatccg ctcggtggtg acgaggcgca ccggcacacg
    13501 ggcgagaaag gcgccgagcc tgcccttcgc ctcgaacgcg cggcgaaagc ccgctcgatc
    13561 gaagagcggg ccgagcttcg ggcagagacc gcccgagaca tagacgccgc ccttggcgca
    13621 gaaggtcagc gccagatcgc cggcggcggc acccagcatg gcgctgaagc gctggagggc
    13681 cgcgaccgag acggggcaac tgccgtcgcg tgcggcccgg gtcacagttt cggggctcgt
    13741 cgccgcgagc tcgctcgagg tgagcgccgc gagcgactcg gccagagcca gaagacccgg
    13801 ccccgaaagc agccgctcgt tggagacgtg cccgtgccgg gcccgcagat ggcgaagcag
    13861 ctcgatctcc tggtcgtcct gcggcgcaaa cgagacgtgc cctgcctcgc tcgccacgac
    13921 gatcgggcca tccggccccg gcaccagcgc cgcggcaccg agcccggtgc ccgggccgat
    13981 cacggcaatg ggccagtcgg gatccgcctc gccaccaccg atcgcaacgc tgtcggcggg
    14041 acccagatac gggattgccg cggcctgggc ggcgaaatcg ttgatcacga ccagcctggc
    14101 gatgccgagc tcggcctgga gctggtcgcg ggtgaaggtc cacgggctgt tggtgaagcg
    14161 gatcgtctcg gcgtcgaccg gcgtggccac cgcgatgacc gcctcgtcga tcgaccctgg
    14221 cggctcgaac agggccgcca gataagcctt ggcggcttca ccgggcccgg catgcgccgc
    14281 cacggccagg cgtcgctcgc ggctcggcat gcccactgcg tcggtcagcg cgaaccgggc
    14341 attggtgccg ccgatatccg caatcagcct cgtcatcctg gcatcctttc gccctaacct
    14401 gccgaggcgg acagccccgc gtcaagcggc gccacgcaac gaagcggctg tctccggctc
    14461 ccgcatatgc tatgagaatg gcgccgcgat ctgacggtgt ggatggcgat gcgcaaaccc
    14521 ggggatcaca acgagatcga tacgaggctc gccagcctgc gcgcggcgca tcgcgcgctc
    14581 gactgccgca tcggccagct caccgacgag agcttcgtcg atcaggtggc gttgcagcgg
    14641 ctgaagaagc agaagctgat cctcaaggac cgcatctcgc agctcgagag cgaccgctac
    14701 cccgacatca tcgcctgaag cgcgtgacat ggcccatctt gcggcccgga cgtgcctcgc
    14761 gcttgccgta gaggtggagg caggcacccg gctccgccag gatcgccggc caggcgtcga
    14821 tcgcatcgcc caggaggttc tgcatcacgc aaggcgccag cggcgtggtg tcgccgagcg
    14881 gcaggtcgag gatcgcccgc acctgctgct cgaactggct gaccgtgcag ccgtcgatgg
    14941 tccagtgccc cgaattgtgc gggcgcgggg cgagctcgtt gacgaggagg cgacccccgg
    15001 gctcgaggaa catctcgacg gcgagcaggc cctcgaggcg aagggcggcg gcgatccgct
    15061 cggcgatgtc gatcgccgag cgggcgacgg atcccggcag ctcggccggg gcgatggtct
    15121 cgacgaggat ctgcgcctgg tggcggttct cgaccggcgg gaagcagcgc atctcgccgt
    15181 tcgggcgacg ggcgacgatc accgagagct cgcgctcgaa cggcacgaag gcctcgagga
    15241 tcagggcttc gccacccagc cgggtaaagg cgtccgccga gcggctctcg ggcccgagca
    15301 cctcctgccc cttgccgtca tagccgaagc gccgggtctt gagcaccgcc cggggaccga
    15361 gctggtcgag gccggccatg agcgcatcga catcgtcgac ccgggcgaac ggcgccacgg
    15421 gcacgtccag gccggcgacg aaggacttct ccaggaggcg gtcctgggtg atgcgcagca
    15481 cctcggctcc cggccgcatc ggcttctgcg cggcgatggc ggcgagggcc gagaccggca
    15541 cgttctccca ttcgagggtg acgacatcga ccacggcgag gaattcggcg agcgccccgg
    15601 agtcgtcgta accggcctgc cagtggcgcc atgcgacgtc ggcggccggt gcctcggcct
    15661 ccggcgcgag caccgccgcg cggcagccga ggcgggcggc ggcgagcgcc gtcatccgcc
    15721 cgagctgacc gccgccgagc acaccgatcg tggcacccgg ggcgaggctc atgccgggcc
    15781 tgcgggctgg acatccgccg ggtcgccgtc gctcgggcgc tcggcgaccg ccgccgtctg
    15841 cgcgtcccgg taggccctga gccgctgcgc cagcccggaa tcggtggtcg cgagcatggc
    15901 cgccgcgagc agccccgcat tggtcgcccc ggcggcaccg atggcgagcg tgccgaccgg
    15961 cacgccggcc ggcatctgga cgatcgagag caggctgtcg aggccgctga gcgcccggct
    16021 ctcgacgggc acgccgagca ccggcaagtc ggtcatcgcc gccaccatgc ccggcagatg
    16081 ggccgccccg ccggccccgg cgatcaccac cctgaagccc tcccccgcgg cgccgctggc
    16141 gaacgcgacg aggcgctcgg gcgtgcgatg cgccgagacg atgcgtgtgt cgcaggcgat
    16201 gtcgagctcg cgcaggacgg ccacggcgtg ccgcatggtc ggccagtcgg actggctgcc
    16261 catgatgacc gcgaccggca cggcaccaat catcgggccc gccctcgctc agtcgccatc
    16321 ggggtcggca tccgccaccg tgccgggctg cctgtcgccg ccgcgcaagg cgccgaccgc
    16381 ggcggcggcg gcctcgttct cgcgcttgat ctgcaggtag atctcctcgc gcaggaccgt
    16441 ggcgctcggc gggaaggtga agccgagctt gaccgtcttg cccttcagct cgacgacccg
    16501 gacctcgatc gcgttgttga tgatcaccgc ctcgccgatc cgccgcgtca gatagagcat
    16561 gccggcctcc tgcctgcgtt cccggccggg gtggcggtcg ggatttaagg gtctgttaac
    16621 cgtaagcccc tatcgtcgca agcgttcgac ccctgcaacc gagcggagaa cgccatggcc
    16681 gcccccctgc ccgccctgtt tcagctgctg tccgcccggg ccggctttct ccaggcgcgc
    16741 cacgcggtcc tcgcccagaa cgtcgccaat gccgacacgc cacgctacca gccgcaggat
    16801 ctggtcgagc ccgccttcga agcgctgctg cggggcggcc cgggctcgac gccatcggtc
    16861 gccgtggcgc ggacgcgatc ggcccacctg gctggcgcct cccccggggc ggccagcccg
    16921 cgccagaggc ccatcgaagc cttcgagacc gccccggccg gcaacgcggt catcatcgag
    16981 gagcagatcc gcctgatggg ccaggcggat cgcggctacc agctcgcgac gaatctctac
    17041 ggcaagtatg tcggcatgat gcgcaccgcc ctgggcgccg gcggcgcctg acgcggtatc
    17101 gggcccgcga caagagcccc cgaggagcag ccatggatct cgagacctcg atgcggatcg
    17161 ccgccgccgg gatgcaggcg cagtctgccc ggttgcgggt cactgccgaa aatctcgcca
    17221 atgcgcaatc cacggcggag agcccgggcg gcgaccccta ccagcgcaag accatcagct
    17281 tcgccaacgc gctcgatcgg gcgaccggtg cccggctggt cgaggtgcgt cgctacggca
    17341 ccgacccggc agccttcgag cgccgcttcg agccctcgca tccggccgcc gacgcctcgg
    17401 gatacgtgct ctacccgaac gtcaacccgc tgatcgagct catggacatg cgcgaggccc
    17461 agcggtcctt cgaggccaat ctcacggcgt tgcagcaggc gcgcggcatg ctgcaacgca
    17521 cgctcgacct cctgcgttga gccggatcgc gccgcgccac accgcatcgg cacctgaccc
    17581 gcaaccggag acggcacaat gagcatcggc gccgccaacc aggccctcgc cgcctatcgc
    17641 gccgcggcca gcgagatcgc cggcggcaag caagcgacca cggccgcagg catcggatcc
    17701 ttcgacggcc tgctccaggc cgagctgcgg agcacgctcg ccacgctcga ggcgagcgag
    17761 caggcgagca tcggcgcggt ctccggccgg gtcggcatcc agcaggtggt ggaggcgatc
    17821 accgccgccg agctcagcct gcagaaggtg acggccgtcc gcgaccgggt gatctcggcg
    17881 taccaggaaa tcatccggat gccgatctga gcgcgaatgg cgatgcggat gacgggaacg
    17941 gcccgatgct gaccgacctc atggtgcagg acgtcgcccg tcaggcgatc tgggtgatgc
    18001 tgaaggtcgg cgctcccatg atgctcgtcg cgctcgtcgt cgggctctgc gtttcgctgc
    18061 tccaggccct gacccagatc caggagatga cgctcacctt cgtgcccaag atcctcgcca
    18121 tgttcatgac cct
//
LOCUS       DAOTFI010000088        18108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3719_length_18108_cov_7.547222, whole genome shotgun sequence.
ACCESSION   DAOTFI010000088 DAOTFI010000000
VERSION     DAOTFI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18108
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3719_length_18108_cov_7.547222"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="P5558_10670"
     CDS             <1..169
                     /locus_tag="P5558_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRY24815.1"
                     /translation="SHAGIAHTMFQTLADRGINIQAISTSEIKVSVLIDEAYTELALR
                     ALHTAYGLDGS"
     gene            173..1219
                     /locus_tag="P5558_10675"
     CDS             173..1219
                     /locus_tag="P5558_10675"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRY24816.1"
                     /translation="MTHLPPHPRLQALWRRGAGLLGTETAILAGAMTWVSERHLVAAI
                     GEAGGFGVLACGSMTPDLLAREITATKALTDRPFGVNLIVMHPALDRLVEVCMDLRVG
                     HVVLAGGLPPGAAIAKIKGGGSRLLCFAPSLALGRRLARSGADGLIIEGMEAGGHIGA
                     ISTAVLAQEILPFLTEVPVFIAGGIGHGEAIAAFLRMGASGVQMGTRFACAVESIAHP
                     KFKKAFIRAAARDAQPSVQVDPEFKVIPVRALANNGTRRFAQTQVEVIGRYRRGELSL
                     EEASLAIEHFWAGALRRAVVEGDVEEGSLMAGQSVGFVKAEQPVAEIMAELVDQAEAA
                     LGRADAGPLAAVGA"
     gene            1222..>2129
                     /locus_tag="P5558_10680"
     CDS             1222..>2129
                     /locus_tag="P5558_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRY24817.1"
                     /translation="MAAPRRGGEASAERRSAELTGARQLLRRLVEVVALPIDPQARLD
                     RIVSLIAANLVAEVCSIYALRAGEMLELFATEGLAREAVHRTRMAVGQGLVGTIAAAG
                     TLINTDDAQHHPNFRFFPETGEDVYNSFLGVPVVRTGRVVGVLVVQNTARKKYAEDEV
                     EALQIIASVLAEMLASGDLVDVDRYTDLPGLGSEQRRLEGRRLVEGLAIGPAWLHAPK
                     IEITRLLADDPVAEAARLEAAVESLRESLDAMLERTDLGAGEHREVLETYRMFAHDGG
                     WRRRMREAIETGLTAEAAVKRVQEDT"
     assembly_gap    2130..2229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2230..3707
                     /gene="ptsP"
                     /locus_tag="P5558_10685"
     CDS             <2230..3707
                     /gene="ptsP"
                     /locus_tag="P5558_10685"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRY24818.1"
                     /translation="ETGLTAEAAVKRVQEDTKLRIGHASDAYLRERLLDLEDLAERLL
                     RLLAGRDSRHEAGMMPPGSIIVARNLSAADLVDYDQSKIGGIVLEEGSATAHVTIVAR
                     AFDVPMLGRVSRAMAQIEAGDLLALDAEHGQVFLRPSDEVVEAFKDATRAREERRRLF
                     GSLKGEPSVTADGMPVKLSINAGFLIDLGELDTTGAEGVGLFRTELSFMSRPDFHDPR
                     VQAEYYATVLDRAGGRPVCFRTLDIGSDKQLPYFRMPAEENPAMGWRALRLTLDRPVI
                     LRHQLRALLEAAAGRPLLRVMFPMVAEVAELRAARHLLELERRRAEARGQALPERIEV
                     GAMMEVPSLYWQLPVLLREIDFLSVGSNDLLQFLFACDRGSQVLGDRYDVLSPPALGF
                     LRGLVEHCRAAGVPLAVCGEMASRPIEAMALVGLGVRRLSLSPAQIGPVKVMLRSLDA
                     GRLSDYLHGLLDLPDHSLRRRLLAYAHDHGVALPDHTFRAI"
     gene            3870..5102
                     /locus_tag="P5558_10690"
     CDS             3870..5102
                     /locus_tag="P5558_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRY24819.1"
                     /translation="MSRRAPSPEPAIDTSAQRLVAVGRHLREARQQRGEDLYDVADYL
                     RIKPSYLFALEEGDLSAMPGRAYAMGFLRSYADHLGFDGTEVVHGLRVGLAADAEQEL
                     NVRQPAAENRLPSLSLVVLGLVLGGLAYGGWYAVQAGGGDVLQRVRALPGEIGQYAAT
                     LFDDAEAPPRVVEPTAGDRPAPAQPTVTAAVPAPPESPKPVQGLPETAPVSGVIARTA
                     PPAGAVVAGADGQPPAPGLRPSEAYVPEAAAAEPPAAGPLADLVLDAEAERAAAVRRQ
                     DDAPAEPDTAGDLLAALEVPAAEPDADASAPASGTLFGAGAADGAGSDGRVVLLAREA
                     SWIQVQSPARDYVTSRTLEPGDRFVIPDRNDLALWTGNAGGLEVWVDGEKVGTLGRSG
                     AVVRDVSLAPDSLLTRLR"
     gene            5108..6346
                     /gene="ispG"
                     /locus_tag="P5558_10695"
                     /gene_synonym="gcpE"
     CDS             5108..6346
                     /gene="ispG"
                     /locus_tag="P5558_10695"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRY24820.1"
                     /translation="MVALAQVHRRSTTPVRVGTVTIGGGAPVVVQSMTNTDTSDLDAT
                     CRQVIELAQAGSEIVRVTVNDEPAARAVPRLRERLDLLGHSVPLVGDFHYNGHRLLAA
                     VPELGASLAKLRINPGNVGFGDKRDRQFEAMIEAALVAGRPVRIGVNWGSLDQALAAR
                     LMDENASSPVPESADFVLREALVRSALESAAHAERMGLGADRIVISAKVSRVPDLIAV
                     YRELARRSTYALHLGLTEAGMGSKGIAASAAALSVLLTDGIGDTIRCSLTPRPGESRT
                     TEVRVCQEILQSLGLRSFAPEVAACPGCGRTTSTFFQELALRIETYLARQMPVWRASY
                     PGIEGLKVAVMGCIVNGPGESKHADIGISLPGTGESPVAPVFIDGAKATTLRGEHIAE
                     EFQALVERYVEERFGERPAA"
     gene            6380..7618
                     /gene="hisS"
                     /locus_tag="P5558_10700"
     CDS             6380..7618
                     /gene="hisS"
                     /locus_tag="P5558_10700"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRY24821.1"
                     /translation="MATARPVRGTHDLFGETIRRHRHVIETGRAVSALYGYEEIATPV
                     FEFTEVFLRTLGDTSDVVTKEMYTFEDRGGESLTLRPENTAGVARAAISNGLLQGLPL
                     KFFYAGPMFRYERPQKGRMRQFHQIGIELIGAPEPQGDVEVIAVGAMILERLGVAGHV
                     TLELNTLGDPASRQAYREELVRYLEGYRDRLSEDSLARLGRNPMRILDSKDEGDRQVV
                     EGAPRLEDSLNDASRDFFEAVRDGLDELGLAYRLNPRLVRGLDYYTHTAFEFTTDQLG
                     AQGAVIAGGRYDGLVEEIGGPPTPGVGWAAGVERLALLAGREEVRARPVAIVPIGPAA
                     EKAARLLAAELRSAGIAVELAFKGKPGQRMKRADRAAARVALSLGDDELASGVVKLRD
                     LDSGEEQVLPRSELVRHLAV"
     gene            7710..9416
                     /locus_tag="P5558_10705"
     CDS             7710..9416
                     /locus_tag="P5558_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRY24822.1"
                     /translation="MTATGFLIELLGGIALLLWGVRMVRTGVSRALGSSLHRLLRAAA
                     GNRLSALGAGFLVTVPLQSSTATALIVGAFAERGLIALAAALAVMLGADIGTAVVAQL
                     LAIDLHWLSPISIIVGFGCFVVGPTTRVRQLGRALIGVGLIMLALRLVVASSEPLRTS
                     PALAAVLTALEDDIVFTAALAALLTWLAHSSIAVILTVAGLVGVGVVPPAMALAVVLG
                     ANAGGAIAPFVATSASPPPGRRPALGNLVMRGGGAVLGLAALPWLAPLILAHLGSGTG
                     LVLNLHLAFNLLMAILMLPLVGLVARLVTRLLPDRPTAADELLRPRHLDKEALQEPSV
                     ALANAAREALRLGDAVEAMLRQTIDAFAPGSTPELVKKIEAEDDVVDALHEAIKLYLT
                     KLSQSELEPEESHRCFEILTFTTNFEHIGDIIDRNLMELATKKLKAGVRFSDEGFAEI
                     ERFHARIVASMQMALSLFMRGDLVEARRLIAEKTELREMEAAAAESHLSRVGRRLPQS
                     LQTSSLHIDVIRDLKRIHSHLTAVAYPILERAGELSPTRLRRPADASTSAAESPAAVP
                     GH"
     gene            9475..10557
                     /gene="prfA"
                     /locus_tag="P5558_10710"
     CDS             9475..10557
                     /gene="prfA"
                     /locus_tag="P5558_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRY24823.1"
                     /translation="MTTRQGGGLDVKLDAILARRDELASLMAAGGGDYAGLGREFAEL
                     EPIVSGIERYRAVQAEIGDLTEMLDDARTEPEMRGMAKDELAGLEGRLSELERELQLL
                     LLPRDAADDASAILEIRAGTGGDEAALFAGDLWRMYERYAQAQGWRTEILSLSETEIG
                     GVKELVAEVQGRGVFARLKFEAGVHRVQRVPVTETGGRIHTSAATVAVLPEAEDVDLE
                     LDEGELRVDVYRASGAGGQHVNKTESAVRITHLPTGIAVAMQDEKSQHKNRAKALKVL
                     KARLFELERSRLAAERSAERKGQVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLDQV
                     MAGEALDEVVSALIAEDQAARLAELA"
     gene            10554..11405
                     /gene="prmC"
                     /locus_tag="P5558_10715"
     CDS             10554..11405
                     /gene="prmC"
                     /locus_tag="P5558_10715"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRY24824.1"
                     /translation="MTTVEGLVAELAALLRAAGIENARGEARLLVEAATGLDRTAQLR
                     TPDRPVETPAAHALAVRRAAREPMAYILGRREFWGRDFAVGPGVLVPRPETETLVEAV
                     LEALPDRRAALRLVDLGTGSGCLLLTLLDLYPAAFGIGVDRSPAALRYAAINRVALGL
                     ERRAALVQGDWLQALAGPFDLVVANPPYIGSGEARDAETRHEPEAALIAGRDGLDAYR
                     AIVGAAASALRPGGLVALEIGWAQAPAVTDLARSAGLGEVGVRADLAGRARVVIGRRA
                     MELSPAP"
     gene            11467..12582
                     /locus_tag="P5558_10720"
     CDS             11467..12582
                     /locus_tag="P5558_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY24825.1"
                     /translation="MLRRIIGIPVILAVMALAGCDESPGDAATAPPPGVGVAPAVRRD
                     VTPTLELVARVEAINAVDLEARVKGFLVQRDFVEGAAVEADQLLFQIEREPYEIAVAA
                     RTADVERMAATLLNAQQQRERMAELVRRNAQPQARLDEATAAEDEARAARDAAVAALR
                     QAELELGYTEIHAPFAGRIGRAAFARGAVVGPNSGPLARLVQLDPIFVAMPVTDRALL
                     AVRRAQDEPESHYQPFLRLADGSVLDEPGTFAFFDPQVSPTTSTITVRASFANGDGLL
                     LPGQFVTVLIRTAEPTQALVIPQVAVQQDQAGTFVLVVDSENRVREARVALGDRVDTG
                     WIVEGGLEEGQTVIVEGLQKVRPGMIVAARAADTGEG"
     gene            12589..15717
                     /locus_tag="P5558_10725"
     CDS             12589..15717
                     /locus_tag="P5558_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRY24826.1"
                     /translation="MLSAFFITNTRFAMVISVVIVIAGLIAMQVLPVAEFPEIVPPQV
                     QVTASYPGASAEVVTETVAAPIEAEVNGVDDMLYMTSTSSNDGGYSLAVTFAVGTDPD
                     IAAVNVQNRVALAAPQLPPEVTGQGVSVKKQSTSMLMVVNLLSPEGSFDRLFLSNYME
                     INLRDAMVRLPGVGDASQFGGQDYSMRIWLDPQVMTALAVSEGEVAAAIRQQNVQAAA
                     GRIGAPPGNEGRAFTYTLQARGRLADPAEFAQILVRANPDGSVVRLGDVARIELGAQS
                     YDAAAFIDGSPTAMLGIYLSPGANALATADAVYAALDRLAERFPDDVEYSILYDTTDF
                     VRISMREVVVTLFQALGLVILVTFLFLGDWRATLVPTLAIPVSLIGTFAVLLAAGMSI
                     NTISMFGLILAIGIVVDDAIVVVENTQRLIDDEKLSAADAARKAMVQVTGPVVATTLV
                     LLAVFVPTAFLPGITGQLYLQFAVTISVAVVLSSLVALTLSPAMCALLLRPSGTPRGP
                     LRWFFAFLDTTRNGYGRVVTVLGRRAFVAVLVVVAAGLGTFWLLRVVPTGFIPYEDNG
                     AFFIDVSLPDAASLGRTEATLTEVEKILLADEDVAHVLSVAGFSLLAGPRSSGGLAIA
                     ILEDWDDRPGRERSVGGVLRRLQPQLLALPQASVFAFPAPPIQGLGTAGGIEMELQDL
                     GGRAPDELARALQAFILRANEHPDIASAFSTFSAQTPQLFLDIDRDKAETLGVRINDL
                     FTTLQANLGTLYINDFNLFGRTYRVFLQAEAPFRMTPGDISALHVRNATGEMVPLGAV
                     LTVQPVLGPEATRRFNLFRSASVNVTPVGSTGDGIAALREVEATALPQGFAIDWTGTT
                     FQEVQAGGEAAIILLLALLFVYLFLVAQYESWTVPLSVILSVAIAGVGGLALTWAVGL
                     DNNVYTQIGLVMLIGLASKNAILIVEFAKQLREEGRPIQEAAADAARIRFRAVLMTAF
                     SFILGILPLVLATGAGAASRVALGMVVMGGMLAATFIGIIIIPPLYVFVQGLRERVRG
                     APAPSTADGRAD"
     gene            15840..>18108
                     /gene="clpB"
                     /locus_tag="P5558_10730"
     CDS             15840..>18108
                     /gene="clpB"
                     /locus_tag="P5558_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRY24827.1"
                     /translation="MDFQNYTERARGFIQSAQGLALREGHQRFLPEHILKVLLDDPEG
                     LASNLIQAAGGNPKAAAQATALLLAKQPKVEGSGAGQVYLAPETARLFESAEQVAKKA
                     GDSFVTVERLLQALAMSATDAGKVLKDAGATAQGLNAAIDAVRQGRKAESASAEDSYE
                     ALKKYTRDLTAIAREGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAE
                     GLALRIVNGDVPESLKDRRLLSLDLGALIAGAKYRGEFEERLKAVLQEITHSQGEIVL
                     FIDELHTLVGAGKAEGAMDASNMLKPALARGELHCVGATTLDEYRKHIEKDAALARRF
                     QPVFVAQPSVEDTVSILRGLKEKYEMHHGVRITDAAIVGAAVLSNRYIADRFLPDKAI
                     DLVDEAAARKRMAIDSKPEEIDELDRRILQLKIEREALKKEQDEPSRQRLGKLEKELA
                     ELEQESSTLTARWQGEKEKLAGATKLKEQLDQARHDLVEAQRRGDYTKAGELTYSVIP
                     GLEKAIAEAEGTQSERMLHEEVTEGDIAQVVSRWTGVPVDKMLEGEREKLLEMEKQLE
                     KRVVGQDAAVQAVSNAVRRARAGLQDPNRPIGSFLFLGPTGVGKTELCKTLAAFLFDD
                     EQAMVRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGALTEAIRRRPYQVILFDEIEKAH
                     PDVFNVLLQVLDDGRLTDGQGRTVDFKNTIIVLTSNLGSEYLAALPEGASAEDARSEV
                     MEVVRASFRPEFLNRL"
BASE COUNT         2508 a         6262 c         6541 g         2697 t
ORIGIN      
        1 cagccatgcc ggcatcgccc acaccatgtt ccagacgctg gccgacaggg gcatcaacat
       61 ccaggcgatc tcgacctccg agatcaaggt cagcgtgctg atcgacgagg cctataccga
      121 gctcgccttg cgtgcgctgc acaccgccta cgggctcgac gggagctgac ggttgacaca
      181 tctgccgccc cacccgcggc tgcaggcgct ctggcgtcgt ggcgccgggc tgctcggcac
      241 cgagacggcg atcctggcag gggccatgac ctgggtctcg gagcgccacc tcgtggcggc
      301 gatcggcgag gccggcggct tcggcgtgct cgcctgcggc tcgatgacgc ccgatctcct
      361 ggcccgggag atcacggcga ccaaggcgct caccgaccgg cccttcggcg tcaacctgat
      421 cgtcatgcac ccggcgctcg accggctggt cgaggtctgc atggacctgc gggtcggcca
      481 tgtcgtgctg gccggcggcc tgccgcccgg tgccgccatc gccaagatca agggcggcgg
      541 cagccggctc ctgtgcttcg cgccgtcgct ggcgctcggc cggcggctgg cgcgctccgg
      601 cgccgacggg ctgatcatcg agggcatgga ggcgggcggg catatcggcg ccatctcgac
      661 cgcggtcctg gcccaggaga tcctgccgtt cttgaccgag gtgccggtct tcatcgccgg
      721 cggcatcggc catggcgagg cgatcgcggc cttcctgcgc atgggcgcct cgggcgtgca
      781 gatgggcacg cgcttcgcct gcgcggtcga atcgatcgcc caccccaaat tcaagaaggc
      841 cttcatccgg gccgccgccc gtgacgccca gccctcggtg caggtcgacc ccgagttcaa
      901 ggtcattccg gtcagggcgc tcgccaacaa cggcacgcgc cgcttcgccc agacccaggt
      961 cgaggtgatc ggccgctacc ggcggggcga gctgagcctc gaggaggcgt cgctcgccat
     1021 cgagcatttc tgggccgggg ccctgcgccg ggcggtggtc gagggcgatg tcgaggaggg
     1081 ctcgctgatg gccggccagt cggtcggctt cgtcaaggcg gagcagcccg tggcggagat
     1141 catggccgag ctggtcgacc aggccgaggc cgctctgggg cgggccgacg cggggccgct
     1201 cgcggccgtc ggcgcctgac cgtggcggcc ccccggcgcg gcggggaggc ctcggccgag
     1261 cggcgcagcg ccgagctgac cggggcgcgg cagctcctgc ggcggctggt cgaggtcgtc
     1321 gccttgccga tcgacccgca ggcccgcctc gatcgcatcg tgtcgctgat cgccgccaac
     1381 ctggtggccg aggtctgctc gatctatgcg ctgcgcgccg gcgagatgct cgagctcttc
     1441 gccaccgagg gcctcgcccg cgaggcggtg catcgcaccc gcatggcggt cggccagggg
     1501 ctggtcggca cgatcgccgc cgccggcacg ctgatcaaca cggacgacgc ccagcatcac
     1561 ccgaatttcc ggttctttcc ggagaccggc gaggacgtct acaactcctt cctcggcgtg
     1621 ccggtggtgc gcaccggccg ggtggtgggc gtgctcgtcg tgcagaacac ggcgcgcaag
     1681 aaatacgccg aggacgaggt cgaggcgctg cagatcatcg ccagcgtgct ggcggagatg
     1741 ctggcctcgg gcgacctggt cgatgtggac cgctacaccg acctgccggg cctcggcagc
     1801 gagcagcgcc ggctggaagg gcgccggctg gtcgaggggc tggccatcgg cccggcctgg
     1861 ctgcatgcgc cgaagatcga gatcacgcgg ctgctcgccg acgacccggt ggccgaggcg
     1921 gcgcggctcg aggccgcggt ggagagcctc agggaatcgc tcgacgcgat gctggagcgc
     1981 accgatctcg gggccggcga gcatcgggag gtgctcgaga cctatcgcat gttcgcgcat
     2041 gacggcggct ggcggcggcg gatgcgcgag gcgatcgaga ccggcctgac cgccgaggcc
     2101 gcggtcaagc gggtccagga ggacaccaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnt cgagaccggc ctgaccgccg aggccgcggt caagcgggtc caggaggaca
     2281 ccaagctgcg catcggccat gccagcgacg cctatctgcg cgagcggctg ctcgatctcg
     2341 aggatctggc cgagcggctg ctgcgcctgc tcgccggccg cgacagccgg cacgaggccg
     2401 ggatgatgcc gcccggctcg atcatcgtcg cccgcaacct ctcggccgcc gatctcgtcg
     2461 actacgacca gagcaagatc ggcggcatcg tgctcgagga gggctcggcg accgcgcacg
     2521 tgacgatcgt cgcccgggct ttcgacgtgc cgatgctcgg ccgtgtcagc cgggccatgg
     2581 cgcagatcga ggctggcgac ctcctggcgc tcgacgcgga gcatgggcag gtcttcctgc
     2641 gaccgtccga cgaggtggtc gaggcgttca aggatgcgac gcgggcgcgc gaggagcgcc
     2701 ggcgcctgtt cggcagcctc aagggcgagc cgtcggtcac cgccgatggc atgccggtga
     2761 agctttcgat caatgccggc ttcctcatcg acctcggcga gctcgatacc accggggccg
     2821 aaggtgtcgg gctcttccgc accgagctca gcttcatgtc gcgccccgat ttccacgatc
     2881 cgcgcgtgca ggccgagtac tacgcgacgg tgctcgaccg ggccggcggg cggccggtct
     2941 gctttcgcac cctcgatatc ggcagcgaca agcagctgcc gtatttccgc atgccggcgg
     3001 aggagaaccc ggcgatgggc tggcgggcgc tgcgcctgac gctcgaccgc ccggtcattc
     3061 tccgccacca gctccgcgcc ctgctcgagg cggctgccgg ccggccactg ctgcgggtga
     3121 tgttcccgat ggtcgccgag gtcgcggagc tgcgcgccgc caggcatctg ctcgagctcg
     3181 agcggcgtcg ggccgaggcg cgcggccagg cgctgccgga gcggatcgag gtgggggcga
     3241 tgatggaggt gccctcgctc tactggcagc tcccggtgct cctgcgcgag atcgatttcc
     3301 tctcggtcgg cagcaacgac ctcctgcagt tcctgttcgc ctgcgaccgc ggctcccagg
     3361 tgctcggcga ccgctacgac gtgctgtcgc cgccggcgct cggcttcctg cgcggcctgg
     3421 tcgagcactg ccgggcagcc ggcgtgccgc tcgcggtctg cggcgagatg gccagccggc
     3481 cgatcgaggc catggcgctg gtcggcctcg gcgtgcgccg cctgtcgctg tccccggccc
     3541 agatcggccc ggtcaaggtg atgttgcgct cccttgatgc gggccggctg agcgactacc
     3601 tccatggctt gctggacctg ccggaccaca gcctcaggcg ccgcctgctc gcctatgcgc
     3661 acgatcatgg ggtggcactg cccgaccaca cgtttcgcgc gatttgagca ccggccggct
     3721 gggacccgat cggggtttgg ctgtggcggt gtggtttgct atggaggact cgcctcgggt
     3781 ggtaacccag cgctgagctc gctccgcagt gtgcaactct gggttcgagg cttcgtctcg
     3841 gcccgcctgt tcaggacgtt tgccaaaccg tgtcccgtcg cgcgccctct cccgagccgg
     3901 ccatcgatac ttccgcccag cggctcgtcg ccgtcggccg ccatttgcgt gaggcgcgcc
     3961 agcagcgcgg cgaggatctc tacgacgtcg ccgactatct caggatcaag ccctcctatc
     4021 tcttcgcgct cgaggagggc gatctttcgg ccatgcccgg ccgggcctat gcgatgggct
     4081 tcctgcgcag ctatgccgat cacctgggct tcgacggcac cgaggtggtg catggcctcc
     4141 gggtcgggct ggcggcggat gccgagcagg agctgaacgt ccgccagccg gcggccgaga
     4201 accgcctgcc gagcctgtcg ctggtcgtcc tcggcctcgt gctcggcggc ctcgcctatg
     4261 gcggctggta cgccgtccag gccggcggtg gcgacgtgct gcagcgggtc agagccctgc
     4321 cgggcgagat cggccagtat gccgcgaccc tgttcgacga cgccgaggcg ccgccccgag
     4381 tggtcgagcc gaccgccggc gatcggccgg cgccggccca gccgaccgtg acggcggcgg
     4441 tcccggcacc gccggagtcg ccgaagccgg tgcaggggct accggagacc gcgccggtga
     4501 gcggcgtcat tgcccgcacg gcgccgcctg ccggcgctgt ggtcgcgggc gccgacggtc
     4561 agccgcccgc gcccggcctg cggccgagcg aggcctatgt gcccgaggcg gccgcggccg
     4621 agccgccggc cgccgggccg ctggccgatc tcgtgctcga tgccgaggca gagcgggcgg
     4681 ccgccgtccg ccggcaggac gatgcgccgg ccgagcccga cacagcgggc gatctgctcg
     4741 ccgccctcga ggttcccgcc gccgagccgg atgccgacgc gtcggcaccg gccagcggga
     4801 cgctcttcgg cgccggggcg gccgacggcg ccgggagcga cgggcgggtc gtccttctgg
     4861 cccgcgaggc gagctggatc caggtgcaga gcccggcccg cgactacgtc accagccgga
     4921 ccctcgagcc gggcgaccgc ttcgtcattc ccgaccgcaa cgatctggcg ctctggaccg
     4981 gcaatgccgg ggggctggag gtctgggtcg acggcgagaa ggtcggcacg ctcgggcgaa
     5041 gcggggccgt ggtgcgcgac gtctcgctgg cgcccgattc cctcctgacc cggctgcgct
     5101 gaccgagatg gtggccctgg cccaggtgca tcggcggtcg accacgcctg tccgcgtcgg
     5161 aaccgtcacc attggcgggg gcgcgccggt tgtcgtccag tcgatgacca acaccgacac
     5221 ctcggacctc gacgccacct gccgacaggt gatcgagctg gcccaggcgg gctcggagat
     5281 cgtccgggtc acggtcaatg acgagccggc ggcgcgcgcg gtgccgcgct tgcgcgagcg
     5341 gctcgatctc ctgggtcaca gcgtcccgct ggtcggcgat tttcattaca acggccaccg
     5401 cctgctggcg gcggtgccgg agctgggcgc gagccttgcc aagctccgga tcaacccggg
     5461 caatgtcggc ttcggcgaca agcgcgaccg gcagttcgag gcgatgatcg aggcggcgct
     5521 ggtggccggc cggccggtcc ggatcggcgt caactggggc tcgctcgacc aggccctggc
     5581 cgcccggctg atggacgaga acgccagctc gccggtgccc gagagcgccg atttcgtgct
     5641 gcgcgaggcg ctcgtccgct ccgccctcga gagcgcggca catgccgagc ggatgggcct
     5701 cggcgccgac cggatcgtca tctcggccaa ggtgagccgc gttccggacc tgatcgccgt
     5761 ctatcgcgag ctcgcccggc gcagcaccta cgcgctgcat ctcggcctca ccgaggcggg
     5821 catgggctcg aaggggatcg cggcgagcgc tgccgccctt tcggtcctcc tcaccgacgg
     5881 catcggcgac accatccgct gctccctgac gccgcgaccc ggcgagagcc ggaccaccga
     5941 ggtccgggtc tgccaggaga tcctgcagtc cctgggcctg cgcagctttg cgcccgaggt
     6001 ggccgcctgc ccgggctgcg ggcgcacgac cagcacgttc ttccaggagc tggcgctcag
     6061 gatcgagacc tatctggccc ggcagatgcc ggtctggcgg gcgagctatc ccgggatcga
     6121 ggggctcaag gtcgcggtga tgggctgcat cgtcaacggg cccggcgaga gcaagcacgc
     6181 cgatatcggc atcagccttc ccggcaccgg cgagagcccg gtggcgccgg tcttcatcga
     6241 cggggcgaag gccacgacgc tgcgcggcga gcatatcgcc gaggagttcc aggccctggt
     6301 cgagcgctat gtcgaggagc gcttcggcga gcggccggct gcctgacccc cgttttccgt
     6361 tccagaaggt tgaggattca tggccaccgc ccggccggta cgcggcacgc acgatctctt
     6421 cggcgagacc atccgccgcc accggcacgt catcgagacc ggtcgcgccg tctcggcgct
     6481 ctacggctac gaggagatcg cgacaccggt cttcgagttc accgaggtct tcctgcggac
     6541 gctcggcgac acctcggacg tggtgacgaa ggagatgtac accttcgagg atcggggcgg
     6601 cgagagcctg acgctccgcc ccgagaacac cgcgggcgtg gcgcgggcgg ccatctccaa
     6661 cggcctgctg cagggcctgc cgctcaagtt cttctatgcc ggaccgatgt tccgctacga
     6721 gcggccgcag aaaggccgca tgcggcagtt ccaccagatc ggcatcgagc tcatcggcgc
     6781 accggagccg cagggcgacg tcgaggtcat cgccgtgggt gcgatgatcc tcgagcggct
     6841 gggggtggcg gggcacgtca cgctcgagct caacacgctg ggcgacccgg cgagccggca
     6901 ggcctatcgc gaggagctgg tgcgctatct cgagggctat cgcgaccggc tctcggagga
     6961 cagcctggcg cgcctcggcc gcaacccgat gcgcatcctc gacagcaagg acgagggcga
     7021 ccggcaggtt gtcgagggcg ctccccggct cgaggattcg ctcaacgacg cgagccgcga
     7081 tttcttcgag gcggtgcggg acgggctcga cgagctgggg ctcgcctacc gcctgaaccc
     7141 gcggctggtg cgcgggctcg actactacac ccacacggcc ttcgagttca cgaccgatca
     7201 gctcggcgcg cagggggcgg tgatcgccgg cggccgctat gacgggctgg tcgaggagat
     7261 cggcgggccg ccgacgcccg gggtcgggtg ggcggccggg gtcgagcggc tggcgctgct
     7321 cgccggccgc gaagaggtgc gggcccggcc cgtcgcgatc gtgccgatcg gcccggccgc
     7381 cgagaaggcg gcacggctgc tggcggccga gctgcggtcg gccgggatcg ccgtcgagct
     7441 cgcgttcaag ggcaagccgg gccagcgcat gaagcgtgcc gaccgggcgg ctgcccgggt
     7501 cgcgctctcg ctcggcgacg acgagctggc ttccggcgtc gtcaagctgc gcgatctcga
     7561 cagcggcgag gagcaggtgc tgccgcggag cgagctggtc cgccacctcg cggtctgaat
     7621 tcgcgacagg ttcgtgacag aactttgacg gtggccatcg aaccgcgcta agtccggagg
     7681 ctgacgcgga tattcggggt gttggccagt tgaccgccac gggttttctc atcgagctct
     7741 tgggcggcat cgcgcttctg ctctggggcg tgcgcatggt ccgcacaggg gtgtcgcggg
     7801 ccttgggctc gtccctgcac aggctcctgc gggcggcggc cggcaatcgc ctgagcgccc
     7861 tcggtgccgg gtttctcgtc accgtgccgt tgcagagcag cacggcgacg gcgctgatcg
     7921 tcggtgcctt cgccgagcgc gggctgatcg cgctcgccgc ggcgctggcg gtgatgctcg
     7981 gcgccgatat cggcacggcg gtcgtggcgc agctcctcgc catcgacctc cactggctct
     8041 cgcccatctc gatcatcgtc ggcttcggct gcttcgtcgt cggcccaacg acgcgcgtgc
     8101 ggcagctcgg ccgggcgctg atcggcgtcg ggctgatcat gctggcgctc cgcctggtgg
     8161 tggcgtcgtc cgagcccttg cgcacctcgc cggcgctggc cgccgtgctg acggcgctcg
     8221 aggacgacat cgttttcacg gcggccctgg cggcgctcct gacctggctg gcgcattcct
     8281 cgattgcggt gatcctgacc gtggcggggt tggtcggggt cggcgtggtg ccgcctgcca
     8341 tggcgctggc ggtggtcctc ggcgccaatg ccgggggcgc catcgccccc tttgtcgcca
     8401 cctcggccag tccgccgccc ggccgccggc cggccctcgg caatctcgtc atgcgcggcg
     8461 gcggggcggt gctcgggctc gcggcattgc cgtggctggc gccgctgatc ctcgcccatc
     8521 tgggcagcgg caccggcctc gtgctcaacc tgcatctcgc cttcaacctg ctgatggcga
     8581 tcctgatgct gccgctggtc ggcctcgtcg caaggctggt gacccggctc ctgcccgacc
     8641 ggcccaccgc tgccgacgag ctgctccggc cgcgccatct cgacaaggag gcgttgcagg
     8701 agccgagcgt ggcgctcgcc aacgcggcgc gcgaggcgct ccggctgggc gacgccgtcg
     8761 aggcgatgct gcgacagacg atcgacgcct tcgcgcccgg ctcgacgccg gagctggtga
     8821 agaagatcga ggccgaggac gacgtggtcg acgccctgca cgaggcgatc aagctctatc
     8881 tgacgaagct gagccagagc gagctcgagc cggaggagag tcatcgctgc ttcgagatcc
     8941 tgaccttcac caccaatttc gagcatatcg gcgacatcat cgaccgcaat ctgatggagc
     9001 tcgccaccaa gaagctgaag gcgggcgtgc gtttctccga cgagggtttc gccgagatcg
     9061 agcgctttca cgcgcggatc gtcgccagca tgcagatggc gctcagcctc ttcatgcggg
     9121 gtgacctcgt cgaggcgcgc cggctgatcg ccgagaagac cgagctgcgc gagatggagg
     9181 cggcggcggc ggaaagccat ctctcgcggg tcggccgccg gctgccccaa tcgctccaga
     9241 cctcgagcct gcatatcgac gtgatccgcg acctcaagcg catccattcg catctgacgg
     9301 cggtcgccta cccgatcctc gaacgggccg gcgagctgag cccgacgcgg ctgcggcgcc
     9361 cggcggacgc gtcgacgtcg gcggccgaga gcccggcggc cgtgcccggg cattgacgcc
     9421 gggccaaatc tgccgaaaac cgggccttca cacagcatct cttggcgatg tcccatgacg
     9481 acaaggcagg gcggcgggct cgacgtgaag ctcgacgcca tactcgcccg gcgtgacgag
     9541 ctcgcgtcac tgatggcggc gggcggcggc gactatgccg ggctcgggcg cgagttcgcc
     9601 gagctcgagc cgatcgtctc gggcatcgag cggtatcgcg ccgtgcaggc cgagatcggg
     9661 gatctgacag agatgctcga cgatgcccga accgagcccg agatgcgcgg catggcgaag
     9721 gacgagctcg ccggcctcga gggtcgcctg tccgagctcg agcgcgagct gcagctcctc
     9781 ctcctgcccc gggacgccgc cgacgatgcc agcgccatcc tcgagatccg tgccggcacc
     9841 ggtggcgacg aggccgcact cttcgccggc gacctctggc ggatgtacga gcgctacgcg
     9901 caggcgcagg gctggcgcac cgagattctc agcctgagcg agaccgagat cggcggggtg
     9961 aaggagctgg tggcggaagt ccagggccgg ggtgtcttcg cccggctcaa gttcgaggcg
    10021 ggggtgcatc gggtgcagcg cgtgccggtg accgagacgg gcgggcgcat ccatacctcg
    10081 gcggccaccg tggcggtgct gcccgaggcc gaggacgtcg atctcgagct cgacgagggc
    10141 gagcttcggg tcgacgtcta ccgagcctcc ggcgccggcg gccagcacgt caacaagacc
    10201 gagagtgcgg tgcgcatcac ccatctgccg accggcatcg ccgtcgccat gcaggacgag
    10261 aaatcgcagc acaagaaccg ggcgaaggcg ctgaaggtcc tcaaggcccg gctcttcgag
    10321 ctggagcgct ccaggctggc ggcggaacgg tcggccgagc gcaagggcca ggtcggctcg
    10381 ggcgaccgct cggagcgcat ccgcacctac aatttcccgc aaggccgggt gaccgaccac
    10441 cgcatcaacc tcaccctcta caagctcgac caggtgatgg ccggcgaggc gctggacgag
    10501 gtggtctcgg cgctgatcgc cgaggaccag gcggcccggc tcgccgagct ggcgtgacca
    10561 cggtcgaggg cctggtcgcc gagctggcag ccctgctgcg ggcggccggc atcgagaacg
    10621 cgcggggcga ggcgcggctc ctggtggagg cggcgaccgg gctggatcgg acggcccagc
    10681 tccggacgcc ggaccggcct gtggaaacgc ccgcagccca cgccctggcg gtgcgccggg
    10741 cggcgcgcga gcccatggcc tacatcctgg gcaggcgcga gttctggggg cgggatttcg
    10801 cggtcgggcc gggcgtgctg gtgccgaggc cggagaccga gacgctcgtc gaggcggtgc
    10861 tggaagcgct gccggaccgg cgggcggcgc tccggctggt cgatctcggc accggctccg
    10921 gctgccttct cttgacgctg ctcgacctct accccgccgc cttcggcatc ggcgtcgatc
    10981 gctcgcccgc agcgctgcgt tatgcggcga tcaatcgggt ggcgctcggg ctggagcggc
    11041 gggcggcgct cgtccagggc gactggctcc aggctctcgc cggcccgttc gatctggtcg
    11101 tcgccaaccc gccctatatc ggcagtggcg aggcgcgcga cgcggagacc cgccacgagc
    11161 ccgaggcggc gttgatcgcc ggccgggatg ggctggatgc ctaccgggcg atcgtcggcg
    11221 cggccgcctc ggccctgcgc ccgggcggcc tcgtcgctct cgagatcggc tgggcgcagg
    11281 cgccggcggt cacggatctc gcccgatcgg ccgggctcgg cgaggtgggc gtgcgtgccg
    11341 atctcgccgg ccgggcgcgg gtcgtgatcg gccggcgggc gatggagctt tccccggccc
    11401 catgatccgg gtagggcttg gctggcttca tcctgcttgt catcggacgc gaaagggccc
    11461 cgagccttgc ttcggcgcat catcggtatt ccggtcatcc tggcggtcat ggcactcgcc
    11521 ggctgcgacg agtcgccggg cgatgccgcc acagcaccgc cgccgggcgt cggcgtggcg
    11581 ccggcggtgc gccgcgacgt gacgccgacg ctggagctgg tggcccgcgt cgaggcgatc
    11641 aacgcggtcg atctcgaggc gcgggtcaag ggcttcctgg tgcagcggga tttcgtcgag
    11701 ggtgccgcgg tcgaggcgga ccagctgctc ttccagatcg agcgcgagcc ctacgagatc
    11761 gccgtggcgg cgcgcaccgc cgatgtcgag cgcatggcgg cgacgcttct gaacgcccag
    11821 cagcagcgcg agcggatggc ggagctggtg cggcgcaacg cgcagccgca ggcgcggctc
    11881 gacgaggcca ccgccgccga ggacgaggcc cgggcggcgc gcgacgccgc cgtcgctgcg
    11941 cttcgccagg ccgagctgga gctcggctac accgagatcc acgcgccgtt cgccgggcgg
    12001 atcggccggg cggccttcgc caggggggcg gtggtcgggc cgaacagcgg tcccctggcc
    12061 cggctggtgc agctcgaccc gatctttgtc gcgatgccgg tgaccgaccg cgcgctgctc
    12121 gcggtgcgcc gggcgcagga cgagcccgag agccactacc agccgttcct tcggctggcc
    12181 gacggctcgg ttctcgacga gccgggcacg ttcgccttct tcgacccgca ggtcagcccg
    12241 acgaccagca cgatcacggt tagggcgagc ttcgccaatg gcgacgggct cctcctgccg
    12301 gggcagttcg tcacggtgct gatccgcacg gccgagccga cgcaggccct ggtcatcccc
    12361 caggtcgcgg tgcagcagga ccaggccggc acgttcgttc tcgtcgtcga cagcgagaac
    12421 cgggtgcggg aggcgcgggt ggcgctcggc gatcgggtcg acaccggctg gatcgtcgag
    12481 ggcggcctcg aggaaggcca gacggtgatc gtcgaggggc tgcagaaggt gcggccgggc
    12541 atgatcgtcg ccgcccgcgc cgccgacacc ggcgagggct gagatccgat gctctccgcc
    12601 ttcttcatca cgaacacccg cttcgccatg gtcatctcgg tggtcatcgt gatcgccggc
    12661 ctgatcgcca tgcaggtgct gccggtggcc gagtttccgg agatcgtgcc gccgcaggtc
    12721 caggtgacgg cgagctatcc gggtgccagc gccgaggtgg tgacagagac cgtggcggca
    12781 ccgatcgagg ccgaggtcaa cggcgtcgac gacatgctct acatgacctc gaccagcagc
    12841 aatgacggcg gctactcgct cgccgtgacc ttcgccgtcg gcaccgatcc ggacatcgcc
    12901 gccgtcaacg tgcagaaccg cgtggcgctg gcggcgccgc aattgccgcc cgaggtgacg
    12961 gggcaaggcg tgtcggtgaa gaagcagtcg accagcatgc tgatggtcgt caacctgctc
    13021 tcgcccgagg gcagcttcga tcgcctgttc ctctccaact acatggagat caacctgcgc
    13081 gacgcgatgg tccgcctgcc cggcgtcggc gacgcgagcc agttcggcgg ccaggactac
    13141 tcgatgcgga tctggctcga cccgcaggtg atgaccgcgc tcgcggtcag cgagggcgag
    13201 gtggcggcgg cgatccgcca gcagaacgtg caggctgccg ccgggcggat cggcgcgccg
    13261 ccgggcaacg aggggcgggc cttcacctac acgctgcagg cgcggggccg gctggccgat
    13321 ccggccgagt tcgcgcagat cctggtgcgc gccaacccgg acggctcggt ggtgcggctc
    13381 ggcgatgtcg cccggatcga gctgggtgcg cagagctacg atgccgccgc gttcatcgac
    13441 ggctcgccga ccgccatgct gggcatctac ctctcgcccg gcgccaatgc gctggccacc
    13501 gccgacgccg tctacgcggc gctcgaccgt ctcgccgagc gctttccgga cgatgtcgaa
    13561 tactccattc tctacgacac caccgatttc gtgcggatca gcatgcgcga ggtggtggtc
    13621 acgctgttcc aggcgctggg cctggtcatc ctggtcacct tcctttttct cggcgactgg
    13681 cgcgccacgc tggtgccgac cctggccatc ccggtctcgc tgatcggcac gttcgcggtc
    13741 ctgctggcgg ccggcatgtc gatcaacacc atctcgatgt tcgggctgat cctggcgatc
    13801 ggcatcgtcg tcgacgacgc catcgtcgtg gtcgagaaca cccagcggct gatcgacgac
    13861 gagaagctct cagcggccga cgcggcgcgc aaggcgatgg tgcaggtgac ggggccggtg
    13921 gtcgccacga ccctggtgct gctcgccgtc ttcgtgccca ccgccttcct gccgggcatc
    13981 accggccagc tctatctgca gttcgcggtg accatctcgg tggccgtcgt gctctcctcg
    14041 ctcgtggcgc tgacgctcag cccggcgatg tgcgccctcc tgctgcgccc gtcgggcacg
    14101 ccgcgcggcc cgttgcgctg gttcttcgcc ttcctcgaca cgacccgcaa cggctacggg
    14161 cgcgtggtca cggtgctcgg ccgtcgggcc ttcgttgccg tcctggtcgt cgttgccgcc
    14221 ggcctcggca ccttctggct cctgagggtg gtgccgaccg gcttcattcc gtacgaggac
    14281 aacggcgcct tcttcatcga cgtctccctg cccgatgccg cctcgctcgg gcggaccgag
    14341 gccacgctga cggaggtgga gaagatcctg ctcgccgacg aggacgtggc gcatgtgctg
    14401 tcggtcgccg ggttcagcct gctcgccggc ccgcgctcga gcggcgggct ggcgatcgcc
    14461 attctcgagg actgggacga tcgtcccggc cgggagcgca gcgtcggggg cgtgctccgc
    14521 cgcctgcagc cgcagcttct ggctctgccg caggcgagcg tcttcgcctt tccggcaccg
    14581 ccgatccagg gcctcggcac ggcgggcggg atcgagatgg agcttcagga tctcggcggc
    14641 cgcgcgcccg acgagctcgc ccgggcgttg caggccttca tcctgcgggc caacgagcac
    14701 ccggacatcg ccagcgcctt ctcgaccttc tcggcgcaga cgccccagct cttcctcgac
    14761 atcgaccgcg acaaggccga gaccctgggt gtgcggatca acgacctgtt caccacgctg
    14821 caggccaatc tcggcacgct ctacatcaac gatttcaacc tcttcggccg gacctaccgg
    14881 gtcttcctgc aggccgaggc gcccttccgc atgaccccgg gcgacatctc cgccctgcat
    14941 gtccgcaatg ccacgggcga gatggtgccg ctcggcgccg tcttgacggt gcagccggtc
    15001 ctcggcccgg aagcgacccg gcgcttcaac ctcttccgct cggccagcgt caatgtcacg
    15061 ccggtcggca gcaccggcga cggcatcgcc gcgttgcgcg aggtcgaggc cacggcgctg
    15121 ccccagggct tcgccatcga ctggaccggc accaccttcc aggaggtcca ggccggcggc
    15181 gaggcggcga tcatcctgct tctggcgctg ctcttcgtct atctcttcct ggtggcgcag
    15241 tacgagagct ggaccgtgcc gctctcggtc atcctctcgg tcgccatcgc cggggtcggc
    15301 gggctggcgc tgacctgggc ggtcgggctc gacaacaatg tctacacgca gatcggcctc
    15361 gtcatgctga tcggcctcgc cagcaagaac gccatcctga tcgtcgagtt cgccaagcaa
    15421 ttgcgcgagg aggggcggcc gatccaggag gccgcggcgg atgcggcgcg gatccgcttt
    15481 cgggccgtcc tgatgaccgc gttcagcttc atcctcggca tcctgcccct ggtgctggcc
    15541 acgggtgcgg gggcggccag ccgggtggcg ctcggcatgg tggtcatggg cggcatgctg
    15601 gcggcgacct tcatcggcat catcatcatc ccgccgctct acgtcttcgt gcagggcctg
    15661 cgcgagcggg tgcggggcgc gcccgcgccc tccacggccg acggccgggc cgactgactc
    15721 gcgccgtctt gcgcggccgg ggcctctccc cacatagatt ccacgagacg gcgatgccca
    15781 tcggggtcgc tagtggctga tccgctcgca cgagggatcg agaagggaaa gatcgactca
    15841 tggatttcca gaactatacc gagcgcgccc gcggcttcat ccagtcggcc caggggctgg
    15901 cgctgcgcga ggggcaccag cgctttctgc ccgagcacat cctcaaggtg ctgctcgacg
    15961 acccggaagg gctcgccagc aatctgatcc aggcggccgg cggcaatccc aaggcggcgg
    16021 cgcaggcgac ggcacttctg ctggccaagc agcccaaggt cgagggcagc ggcgccggcc
    16081 aggtctacct ggcgccggag actgcccggc ttttcgagag cgccgagcag gtggcgaaga
    16141 aggcgggcga cagcttcgtc acggtcgagc ggctgctgca ggccctcgcc atgtcggcga
    16201 ccgatgccgg caaggtgctg aaggatgccg gcgccacggc gcaggggctc aatgccgcca
    16261 tcgacgccgt ccgccagggc cggaaggcgg agagcgcttc ggccgaggac agctacgagg
    16321 ccttgaagaa gtacacgcgc gatctcaccg ccatcgcccg ggagggcaag ctcgacccgg
    16381 tgatcggccg cgacgaggag atccggcggg tcatccaggt gctgtccagg cggaccaaga
    16441 acaaccccgt gttgatcggc gagcccggcg tcggcaagac cgccatcgcc gaggggctgg
    16501 cgctgcgcat cgtcaacggc gacgtgcccg agagcctgaa ggaccggcgg ctgctgtcgc
    16561 tggacctcgg tgccctgatc gccggggcca agtaccgggg cgagttcgag gagcggctga
    16621 aggcggtgct gcaggagatc acccacagcc agggcgagat cgtcctcttc atcgacgagc
    16681 tgcacacgct ggtcggcgcc ggcaaggcgg aaggggcgat ggacgcctcg aacatgctga
    16741 agccggccct ggcgcggggc gagctgcatt gcgtcggtgc gaccacgctc gacgagtaca
    16801 gaaagcacat cgagaaggac gcggcgctgg cccggcggtt ccagccggtc ttcgtcgccc
    16861 agccctcggt cgaggacacg gtctcgatcc tgcgcgggct gaaggagaag tacgagatgc
    16921 atcacggcgt gcgcatcacc gatgcggcga tcgtcggtgc cgccgtgctc tccaaccgct
    16981 acatcgccga ccgtttcctg cccgacaagg cgatcgacct cgtcgacgag gcggcggcca
    17041 ggaagcgcat ggcgatcgac agcaagcccg aggagatcga cgagctcgat cgccgcatcc
    17101 tgcagctcaa gatcgagcgc gaggccctga agaaggagca ggacgagccg tcgcggcagc
    17161 ggctcgggaa gctggagaag gagctggccg agctcgagca ggagagcagc acgctgaccg
    17221 cccgctggca gggcgagaag gagaagctgg ctggtgccac caagctgaag gagcagctcg
    17281 accaggcgcg gcacgacctc gtcgaggcgc aacggcgcgg cgactacacg aaggcgggcg
    17341 agctcaccta ttcggtgatc ccgggcctcg agaaggcgat cgccgaggcc gagggcaccc
    17401 agtcggagcg gatgctgcac gaggaggtga ccgagggcga catcgcccag gtggtctcgc
    17461 gctggaccgg cgtgccggtc gacaagatgc tcgagggcga gcgcgagaag ctgctggaga
    17521 tggagaagca gctcgagaag cgggtcgtcg gccaggatgc cgccgtccag gcggtgagca
    17581 acgccgtgcg ccgggcgcgg gcggggctgc aggacccgaa ccggccgatc ggctcgttcc
    17641 tcttcctcgg gcccaccggc gtcggcaaga ccgagctctg caagaccttg gccgccttcc
    17701 tcttcgacga cgagcaggcg atggtgcgca tcgacatgtc cgagtacatg gagaagcaca
    17761 gcgtcagccg gctgatcggc gcacctccgg gctatgtcgg ctatgacgag ggcggggcgc
    17821 tgaccgaggc gatccggcgg cggccgtacc aggtgatcct cttcgacgag atcgagaagg
    17881 cgcacccgga cgtgttcaac gtgctgctgc aggtgctcga cgacggccgg ctgaccgacg
    17941 gccagggccg cacggtcgac ttcaagaaca ccatcatcgt gctcacctcg aacctgggct
    18001 ccgaatacct ggcggcgctg ccggaaggtg cctcggccga ggacgcgcgg tccgaggtga
    18061 tggaggtggt gcgggcgagc ttccggccgg agttcctgaa caggctgg
//
LOCUS       DAOTFI010000089        18066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3732_length_18066_cov_7.356282, whole genome shotgun sequence.
ACCESSION   DAOTFI010000089 DAOTFI010000000
VERSION     DAOTFI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18066
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3732_length_18066_cov_7.356282"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1257
                     /locus_tag="P5558_10735"
     CDS             85..1257
                     /locus_tag="P5558_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRY24828.1"
                     /translation="MHRRHPISTRPASLCVTATATAVALLHLSSPASAQDATTVSDET
                     LQEELKARDAVIIELMRRVDELERRLGGKDPSPVEAEAAPTEPTEAPRPVESAEAPPP
                     ASRGLIDVDELSAERALERTLVDVGALLLPAGQSEIAPAVGFSRRDSNFPALVGGQVA
                     NANTERNEFAFDLGLSVGLPFDSQFELNVPFDLVNEQANLNINGGIADSQERTGYGLG
                     DITVGFAKTLLREGEWRPDIVGRVVWDTATGDQFDDGVFLGNGFHQIRGQLVAVKRQD
                     PLAFVGGFTYSYQFEDDDVDLGSEYALSLGAALALSPETSLNVALNQTYSDDIEVNGE
                     KINGSDQLAASFDFGGSAIIAPRTLFRLNTSIGLTDDAPDYTVRATVAYRFNTPFF"
     gene            complement(1238..2041)
                     /locus_tag="P5558_10740"
     CDS             complement(1238..2041)
                     /locus_tag="P5558_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY24829.1"
                     /translation="MLATSSANDQLSTADNVTRVSETLRAVTASGVLRPDPWLALVDS
                     RPFSRVCISRYLCASDALSQQIIAVSIDELTREGVPPESRPSLVIYSVGSVSIEDAAT
                     RADLDRLLACLGPVPVVVLSDRTEPEEARNALAAGAMGFIATSEEAPLMCKALSLVQA
                     GGIFVPGDLVSQWLRQDGRPNITEATPAETSSDGPVLANELTPRQMDVLEILQEGHPN
                     KVIAARLRMTESTVKVHVRQIMRRLNAKNRTEVALLAQRHLATFRRMVC"
     gene            complement(2576..2797)
                     /locus_tag="P5558_10745"
     CDS             complement(2576..2797)
                     /locus_tag="P5558_10745"
                     /inference="COORDINATES: protein motif:HMM:NF018053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HRY24830.1"
                     /translation="MTDLDGFVALVPSAPSPPLDALAADCVRWFDSFPAPSSTDGLAR
                     RRRVGLTPRQDSHLREFGYPFVLEDFPSI"
     gene            complement(3020..3370)
                     /locus_tag="P5558_10750"
     CDS             complement(3020..3370)
                     /locus_tag="P5558_10750"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HRY24831.1"
                     /translation="MRSKLTRRVVMIAGGALALLGLWRKAWAVDLAEAKSAGQLGERI
                     DGFVGVVSADAPPEVRALAGEINAGRRAEYAAIAKRQGVALEVVAQLAGEKLVQRAGP
                     GEWILDADGRWLQR"
     gene            complement(3379..3579)
                     /locus_tag="P5558_10755"
     CDS             complement(3379..3579)
                     /locus_tag="P5558_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HRY24832.1"
                     /translation="MSPSLVASCAALPLLVLLLGCQPTVQLEAPKEPIVINLNVKIEQ
                     EVRVRVERDLDELLEDPELFGG"
     gene            complement(3599..4744)
                     /locus_tag="P5558_10760"
     CDS             complement(3599..4744)
                     /locus_tag="P5558_10760"
                     /inference="COORDINATES: protein motif:HMM:NF023167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HRY24833.1"
                     /translation="MSSVRQRTFWALACIPSSSEYAAARGLAGALLGGWLLLAPSIAV
                     AWFPETVQIGRLGLVHDEWTVVLSHLQLERRPGAPLALSIDRIEGPTGIMPFWASLRL
                     VPGAKGWRLDGVVASGKGELVLRFSGAGHRGELDRATAAIEPLRFEPGALQPVRFWPR
                     IGEMVRDVQGTIDLSLDWQRGPPESADLVFEIAGLTFDTDYGRVERIDGTIRLDRLWP
                     PRTATPQRLRIEGLNLGRLVERLEIEALAIEGIMDAALEFSFTEAGRLYIEQSLLEAR
                     GPGVIRYRPAQPPPLLASQGANVDLLIAALADFRYDAATATVKGFVDGDNSVALQLQG
                     ANPELYEGHPIELNLSLEAPILDLVGAGREVLDLPVRLRRAVQELGR"
     gene            complement(4671..5720)
                     /gene="ppk2"
                     /locus_tag="P5558_10765"
     CDS             complement(4671..5720)
                     /gene="ppk2"
                     /locus_tag="P5558_10765"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRY24834.1"
                     /translation="MATKEAAQDATKEAAQDLVAAGDGVSRETAGADLSANGRGPTPA
                     GGRRSSRAALARHQLAEMKRDPGVVRRLFETGEYPYKSHMSRRSYEAHKLELQAELLK
                     AQSWIKATGQKVVVIFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPSEREQTQWYF
                     QRYVEHLPAGGEIVFFDRSWYNRAGVERVMGFCKPNDYLEFMRQCPEFERMLVRTGTW
                     LFKYWFSVTREEQRRRFASREHDPLKQWKLSPMDLASIDKWEDYTEAKEAMFFYTDTA
                     DAPWSVVKSDDKKRARLEALRHFLYMLPYPDKDEALVHAPDPLIVGSSGHVIGAAAHI
                     LGASLHPEQQRVRRG"
     gene            complement(5817..6392)
                     /locus_tag="P5558_10770"
     CDS             complement(5817..6392)
                     /locus_tag="P5558_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24835.1"
                     /translation="MTKLRFVFAVCALVGAGFLSATSSWAQDGAVIEQTAVSSALVHD
                     VDLTTRQVLLETEDGRFVTVVAPPEVRNLPQVEVGDTVTIGYYGGIVASLVPPGAEPI
                     TTQGDTALMRSEEGERPAGATVTTIDTSVVFQAFDAATGRVTFRPQDGPERSLAVRDP
                     EMRAFVETLKPGDTVQVTLVEVIAMVVDPMP"
     gene            complement(6409..8133)
                     /gene="xsc"
                     /locus_tag="P5558_10775"
     CDS             complement(6409..8133)
                     /gene="xsc"
                     /locus_tag="P5558_10775"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HRY24836.1"
                     /translation="MTLMTPSEAMVETLVAQGVTHVFGIVGSAYMDALDIFEPAGIRF
                     VSVAHEQGAGHMADGYARASGRHGVCIAQNGPGITNFVTSVAAAYWAHSPVVVITPET
                     GSNTQGLGGFQEAEQMPFFERITKYQVHCGHQGRMAELLGRCFDYAMLERGPTQFNIP
                     RDLFYGEADYTIQEPIRVERGPGGPESLDAAAALIADAKFPVILSGGGVVMSDGVGEV
                     IRLAEHLQAPVINTYLHNDSFPQSHPLWCGPIGYQGSKAAMKLLAQADVVLALGTRLG
                     PFGTLPQHGIDYWPKNAKLIQVEANPRRIGLVKQATVGVCGDARAATAAIHERLTTAN
                     RAIAAHGNRDARVAEIGKQKAAWEAELDEWGQADGSPVGPRRALRELEKALPKNAMVT
                     TDIGNICSVSNSYLRFEQPQSFFAAMSFGNCGYAYPTAIGAKVARPDRPAVAYVGDGA
                     WGMSLQETLTAVRENIPAVAVVFNNQQWGAEKKNQIDFYADRYVGTNLENPSFAGIAR
                     AMGANGVTVDSPDQIGDALVEAIASNRPSVLEIMVTQELGEPFRRDALKKPRRLLDKY
                     RDYSVTAE"
     gene            complement(8159..10603)
                     /locus_tag="P5558_10780"
     CDS             complement(8159..10603)
                     /locus_tag="P5558_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY24837.1"
                     /translation="MANDLPASCDVVVIGGGIIGCSIAYHLTKLGLRDVVVLERKRLT
                     SGTTWHAAGLIGQLRASRRMTELAKYTSELLWSLEAETGLSTGFRQNGSISVATHAER
                     FEELKRGASMAKNFGLAVEVLTPGDIASRYPLLRTDDLVGGIFLPKDGQADPANVAMA
                     YAKGARSGGARIFENTAVERILVEQGRAAGVLTREGPIRAATTVLAAGMWSHAIARQV
                     GVAVPLHAAEHFYIVTEPIAGLPRDLPVLRVPDECAYVKEDAGKLLLGAFEPVAKPWG
                     MGGIPDDFEFTTLPDDVEHFEPILEAAIRRLPALAETGIQTFFNGPESFTPDDRYLLG
                     ETAELRDLFVACGMNSIGIQSSGGVGKVLAEWIRDRHMPADLVDVDVRRLQPFQANQR
                     YLRERTTETLGLLYAMHWPHRQYATARGVRRSPFHDRLLALGAVMGEQSGWERPNWFA
                     APDGERDYRYSWGRQNWFEPCAAECRAVRDAVVLFDQSSFTKCLVTGPDALALLNHVS
                     AAEMNVPVGRVVYTQWLNERGGIEADLTVTRTGETEFLVVTTAAGQTRDLAWLRRHVG
                     EGCRCAVVDLTSGLPMLGLMGPQSRALLEAVSGESLGDADFPFATSRELEIGCAVVRA
                     SRLTYVGELGFELYVPAEFAVHVFDVLVEAGSAFGLGMAGYHAMNACRMEKAYRHWGH
                     DISVEDTPLEAGLGFAVGWRKPDFIGREALFRQREAGGPTRRLVQLKLADPAELLHQE
                     EPVWSGSEIVGSITSGAYGHRIDASLGMGYVQLPGGVKDLAPGREFAIEIGWRKVPAE
                     ARLEAFYDPANERIRA"
     gene            10730..11848
                     /gene="ald"
                     /locus_tag="P5558_10785"
     CDS             10730..11848
                     /gene="ald"
                     /locus_tag="P5558_10785"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRY24838.1"
                     /translation="MRVGVPKEIKNHEYRVGLTPASVIEFVAHGHDVVVETGAGTGIG
                     SDDAAYAAAGADIAASAADVFATADMIVKVKEPQAAERAMLRPGQILFTYLHLAPDPE
                     QTTELMRSGATCIAYETVTDSHGHLPLLAPMSQIAGRLAIQAGAYCLERAHGGCGILL
                     GGVPGVESARVTVIGGGVVGENAIMMALGMAADVTVLDRNVDALQHLARRFGPGLNTV
                     YSQRASLDRYVVDADLVIGAVLVPGDNAPKLVTADMVRRMRPGTVLVDVAIDQGGCFE
                     TSHPTTHAEPTYIVDDVVHYCVANMPGAVPRTSTLALNNVTLPYALALADKGYKAALR
                     ENPHFRAGLNVHAGKVTYRAVAEAQGHAFADPLDVLAE"
     gene            complement(11855..12277)
                     /locus_tag="P5558_10790"
     CDS             complement(11855..12277)
                     /locus_tag="P5558_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRY24839.1"
                     /translation="MAELALLFASAFLAATLVPFASEITLAGMAMAGWGGKLTLWATA
                     TAGNTLGSLLNWSLGRWARRWRDHRLFPLRPAQLDRATRHFQRWGIWSLLFAWVPVVG
                     DPLTLVAGLFRTPFLPTLILIGIGKGVRYALVLGLVPG"
     gene            complement(12313..12714)
                     /locus_tag="P5558_10795"
     CDS             complement(12313..12714)
                     /locus_tag="P5558_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24840.1"
                     /translation="MNAGPNGTDRQGEAGLSEAQSLLEQVFASEIRAWRGEAALGPLF
                     WGKGVGVSLLLIVFHASLMWQGLALVQQLSLMAIAAYTVWILVAVWRCAGRADPNWGT
                     LARWLTVAWAANATLVLLFLQLDLVMAAFGG"
     gene            complement(12893..13948)
                     /gene="ugpC"
                     /locus_tag="P5558_10800"
     CDS             complement(12893..13948)
                     /gene="ugpC"
                     /locus_tag="P5558_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY24841.1"
                     /translation="MASLDIDRVEKRFGSTRVLEGIDIAVEDGEFLVLVGPSGCGKST
                     LLALIAGLESVTAGEIRIDGVRVNERHPKDRDIAMVFQSYALYPNMTVAQNMAFGLEM
                     RGLPKAERDVAVADAARLLQIEPLLARKPGQLSGGQRQRVAMGRALVRQPRIFLFDEP
                     LSNLDAKLRVEMRTEIKKLHQRLGATIVYVTHDQIEAMTLATRIAIMNKGVLQQLGTP
                     RELYDRPANTFVATFIGSPAMNLIPGAVCGSKVHLEGGIELPLMAPAPDGPTLLGLRP
                     ESITEAADGATGIELAVEVTEPTGPDTLAVVRIAGTEATARLRADTDVVPGRSAAFTL
                     DMSKASLFHPRTGARLD"
     gene            complement(13955..14845)
                     /locus_tag="P5558_10805"
     CDS             complement(13955..14845)
                     /locus_tag="P5558_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY24842.1"
                     /translation="MAATSAPAAEGGFAGNRLARAVLYAALLVFAFYFLLPLYVMLVN
                     SVKPLAEIQGGNMLALPGAFTTEPWASAWSTAQVGVEPTGLRPYFWNSIKMVVPAVLI
                     STLLGALNGYVLTKWRFPGDRLVFALMLFSCFIPFQIVLIPMARILGSIGLAGTTAGL
                     VLVHVVYGLGFTTLFFRNYYDAFPSELVKAAQIDGAGFFRIFWRILLPSSGPIITVTV
                     IWQFTNVWNDFLFGASFAGFGSTPMTVALNNLVSSSTGVKEYNVHFAGAVLAALPTLI
                     VYIVSGRYFVRGLMAGAVKG"
     gene            complement(14832..15722)
                     /locus_tag="P5558_10810"
     CDS             complement(14832..15722)
                     /locus_tag="P5558_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY24843.1"
                     /translation="MTDARGRLQELLPKLVLAPSFAAVLLFVYGFIFFTGWLSFTDSR
                     ILPSFGWVGLENYVRLFGLRHWTIAVQNLAVFGLLYILFCSAMGLLLAILLDQKIRGE
                     GFLRPIYLYPMALSFIVTGTAWKWFLDPGIGLEQVVRSWGFADFTFGWIKDSSMAIYC
                     IVIAAVWQSTGFVMAMFLAGLRGIDSEIMKAAQIDGASTFQLYRRIIIPQLRPAFLSA
                     FVVLSHLAIKSYDLVIALTGGGPGRATEMPATFMYSYTFTRNQMGVGAASAVIMLMMI
                     ASVIVPYLYAEMREGRHGGH"
     gene            complement(15801..17051)
                     /locus_tag="P5558_10815"
     CDS             complement(15801..17051)
                     /locus_tag="P5558_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY24844.1"
                     /translation="MNKQIRALLAGTTMLMASAGWATAEDVEVLHWWTSGGEAAALNV
                     LKENLEAEGIGWQDMPVAGGGGEQAMTVLRARVTGGNPPTAVQMLGFDIQDWAALGVV
                     ADLNELAAEESWDAVVPAALQDFSKFDGKWVAAPVNVHSTNWVWANKAIFDELGLAQP
                     TSWDELVAALDAIEAAGYTAVAHGGQAWQDATIFDAVVVSTGGPEFYKAAMIDLDPEA
                     LGSDTMKEAFDRMAVIRSYVDDNFSGRDWNLATAMVINKEAGVQFMGDWAKGEFLNAG
                     KVPGEDFLCFRFPGTQGTVTFNADQFVMFEVGEEGRDAQKKLASAILDPGFQIAFNKV
                     KGSVPARTDVAGDELDACGQQGMMELAEAAAAGHLYGSMAHGHGAPAAVKNAVYDVVT
                     AHFNGEYDSATAVEELVAAVEGAQ"
     gene            complement(17200..17550)
                     /locus_tag="P5558_10820"
     CDS             complement(17200..17550)
                     /locus_tag="P5558_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRY24845.1"
                     /translation="MEILLKRAYEPATAEDGCRILVDRLWPRGRSRADLAIDHWPKEV
                     APSTDLRRWYGHDPKKWDEFRSRYDQELDANREVVKDLLGHCRKGRVTLVFGARDVEH
                     SDAVVLRDHLLRLA"
     gene            complement(17566..>18066)
                     /locus_tag="P5558_10825"
     CDS             complement(17566..>18066)
                     /locus_tag="P5558_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY24846.1"
                     /translation="DIGGGGIMDIGCYMTIFSRWMLGAEPKRAVGLIERDPGFGTDRL
                     ASAILDFGDGRRSVWTTSTQLAPAQRFTVLGTKGRLEVEIPCNAPNDRPCRITVDDGR
                     DLVGGGREVIDLPICDQYRLQGEMFSKAVRGEGELAMPLDESIANMKVLDALFASEKS
                     GGWVAV"
BASE COUNT         3101 a         6169 c         5946 g         2850 t
ORIGIN      
        1 ggcctgatcc cgccgaaccg tctggagccg acgctggcgg aattcctcgt gcttcgctga
       61 gcctcatcag aagtggtaaa tttcatgcac cgtcggcatc cgatctcgac acgtccagcg
      121 agcctgtgcg tcaccgccac ggcgacggcc gtcgcccttc tccacctttc cagccccgcg
      181 tcggcccagg atgcaaccac ggtcagcgac gaaaccctgc aggaggagct caaggcacgc
      241 gacgccgtca tcatcgagct gatgcgcagg gtcgacgagc tcgagcgtcg actggggggc
      301 aaggacccgt ctccggtcga ggcggaggcg gcgccaaccg aaccgacgga agctccgcgg
      361 cccgtcgaat ccgccgaagc gccgccgccc gcctcgcgcg gcctgatcga tgtcgacgag
      421 ctctccgcgg aacgtgccct cgagcgcacg ctggtggatg taggcgcgct gctgcttccg
      481 gctggccagt cggaaatcgc tcccgcggtc gggttctcgc gacgcgactc gaacttcccg
      541 gcccttgtcg gcgggcaggt cgccaatgcg aacacggagc gcaacgagtt cgccttcgac
      601 ctggggctga gtgtcgggct gcccttcgat tcgcagttcg agctcaacgt gccattcgat
      661 ctggtcaacg agcaggcgaa tctgaatatc aatggcggta tcgcggattc ccaggagcga
      721 acgggctatg ggctcggtga catcaccgtc ggatttgcca agacgttgct gcgtgaaggc
      781 gagtggcggc ccgatatcgt cggtcgcgtc gtttgggaca cggccaccgg tgaccagttc
      841 gacgacggcg tcttcctcgg caacggcttc catcagattc gcggccagct ggtcgccgtg
      901 aagcgtcagg atccgctcgc cttcgtcggt ggcttcacct acagctacca gttcgaggac
      961 gatgatgtgg acctcggcag cgagtacgcc ctgtcgctcg gcgcggccct ggccctcagc
     1021 ccggaaacgt cactgaacgt ggcgctcaac cagacctatt cggatgatat cgaggtcaat
     1081 ggcgagaaga tcaacggcag cgatcagttg gccgccagct tcgacttcgg cggctcggcg
     1141 atcatcgcac cgcgcacgct ctttcgcttg aataccagca tcggccttac cgacgacgcg
     1201 cccgactaca ccgtgcgcgc cacggtcgcc tatcgcttca acacaccatt cttctgaacg
     1261 ttgccaggtg acgctgcgcc agcagcgcca cctcggtccg gttcttggcg ttcagtcgcc
     1321 gcatgatttg ccggacatgc accttcacgg tgctttcggt catccgcaaa cgggcggcga
     1381 tcaccttgtt cggatgcccc tcctggagaa tctccagaac gtccatctgg cgcggtgtca
     1441 gctcgttggc cagcaccgga ccgtccgagc tcgtttccgc cggcgtcgcc tcagtgatgt
     1501 tcgggcgtcc gtcctgcctg agccactgac tgacgagatc gcccggcacg aagatcccgc
     1561 ccgcctgcac cagggacagc gccttgcaca tcaacggcgc ctcctcggac gtcgcgatga
     1621 agcccatcgc ccccgccgcg agcgcgttgc gtgcctcctc tggctcggtg cgatcggaca
     1681 gcacgaccac cggcaccggg ccgaggcagg ccagcaaccg atccaggtcg gcacgggtgg
     1741 ccgcgtcctc gatcgacacg cttccgacgc tgtagatcac cagcgagggc cggctttccg
     1801 gcggcacgcc ttcacgcgtc agctcgtcga tggatacggc gatgatctgc tggctgagcg
     1861 catccgaagc acagagatag cgcgagatgc aaaccctgct gaacggtcgc gaatcgacca
     1921 gcgccagcca gggatcaggg cgcaggactc cgcttgcggt gactgcgcgg agtgtctctg
     1981 aaaccctcgt cacattgtct gcggtcgaca gttgatcgtt cgctgatgac gtcgccaaca
     2041 tgaccgtctt gctcccctcc gactccggca accgaagtgg tatgcgatgc cggcgtagct
     2101 atgtagctca taaccctacc ggtcgaggca cgcttcaccg gcagagtcct tatgcttaag
     2161 gcgtattaat acaatcgatg aaatggacat cttttgcaag agaggatcgc cctctctcgc
     2221 tgtagaatag tacctacgtt gacgttcaac cctcgggcaa tcgcccaaat gatcgtgcgt
     2281 cttaatcctc tgttagcttc gacacctaga gccaattgaa ccaatggttg tagaacgaac
     2341 acaggcagga gcgactatta gatgggaaac cctgctcgac tcaccgcagc cgtgtctgtt
     2401 cggcgctcac ggaaggcatg gcggctccta tgcatgaagg gatggtcgcg cgaatcctgc
     2461 gcaaaaaatc gcaatcgcgt cgatggccag atcctgtttt tcgatcaatt gggcgtgatc
     2521 gcgcagaaac gggagcggtc gataccgctc tcctcggcaa gtcgccgggt cagcgtcaga
     2581 tggaggggaa atcctcgagg acgaagggat agccgaactc ccgcaaatgg ctgtcctgtc
     2641 ggggtgtcag cccgacgcgg cggcgccggg ccagtccgtc ggtgctggaa ggtgcaggaa
     2701 acgaatcgaa ccagcgcacg caatcagcag caagagcatc cagcggtggc gacggtgcgc
     2761 tcggcacgag ggcgacaaac ccgtcgagat cggtcaccgt caatagcagc gaaatgctcc
     2821 agctcgctcc ccggcgccgg cgagaaacag atcgcatatt gacccactca agcggtccaa
     2881 tccccgggat cgggcaacgc gcttcccccg acaagaaccg cgcaaacgat cggccgaccg
     2941 cggatccgct tcacgcgcaa cagatcggcg cgccgggctc acggacaggt tggctgcatg
     3001 gtcgcaaaga ccagccgggc tatcgctgga gccaccgccc gtcggcatcg aggatccatt
     3061 cccccggccc ggcacgctga accaacttct cgccggccag ttgggccacg acctcgagcg
     3121 ccacaccctg acgtttggca atcgcagcat actcggctcg gcggccggca ttgatctcgc
     3181 cggccagggc tcgcacttcc ggcggtgcat ccgcgctgac caccccgaca aagccgtcga
     3241 tgcgctcgcc caactggccg gcactcttgg cctcggcaag gtccacggcc caagccttgc
     3301 gccagaggcc gaggagggcg agcgcgccac cggcaatcat gacgacacgg cgggtaagct
     3361 tcgagcgcat cacgtgatct agcctccgaa gagctccgga tcttccagga gctcgtccag
     3421 gtcgcgttcg acgcgcaccc gaacctcctg ctcgatcttg acgttgagat tgatgacgat
     3481 cggctccttg ggcgcctcga gctgaaccgt cggctggcag ccgaggagga ggacgagcag
     3541 gggcagggcg gcgcacgagg cgaccaggga tggggacacc gcgagtccgt ggccccgctc
     3601 agcgcccgag ctcctgcacc gcgcgacgca agcggaccgg cagatcgagc acctcccggc
     3661 cagcgccgac gaggtcgagt atcggggctt cgaggctgag attgagctca atgggatggc
     3721 cctcgtaaag ctcgggattg gcgccctgca actgcaacgc gacagagttg tcgccgtcga
     3781 cgaagccttt caccgtcgca gtggccgcat cgtagcggaa gtctgccaag gcggcgatga
     3841 gcagatcgac gttcgctccc tggctcgcca gcaggggcgg cggctgcgcc ggtcgatagc
     3901 ggatgacgcc cggcccacgc gcctcgagca ggctctgctc gatatagagc ctgcccgctt
     3961 cggtgaaact gaattcgagg gcggcatcca taatgccttc gatcgccagc gcttcgatct
     4021 ccaggcgctc gaccaagcgg ccgagattta ggccttcgat ccgcagccgt tggggcgttg
     4081 ccgtccgcgg cggccagagg cgatcgagcc ggatcgtccc gtcgatccgc tcgacccggc
     4141 catagtcggt gtcgaacgtc agcccggcga tctcgaacac gagatcggcc gattccggcg
     4201 ggccccgctg ccagtccagt gagagatcga tggtaccctg cacatcccgg accatctcgc
     4261 caatgcgcgg ccaaaagcgg accggctgca gggcgcccgg ctcgaagcgc aacggctcga
     4321 tggcggccgt cgcgcgatcc agctcaccac gatggccggc cccgctgaag cgaaggacca
     4381 gctcgccctt gccactggca accacgccat cgagccgcca gcccttagcc ccgggaacca
     4441 gtctcaggct ggcccaaaag ggcatgatgc ccgttggccc ctcaattcga tcgatgctga
     4501 gcgccagcgg cgccccgggt cggcgctcga gttgcaggtg ggagagcacc acggtccact
     4561 cgtcatggac cagccccagc cggccgatct ggaccgtttc agggaaccag gccacagcaa
     4621 tcgatggggc aagcagcagc cagccaccga gcaaggcacc ggcgaggccc ctagccgcgg
     4681 cgtactcgct gctgctcggg atgcaggcta gcgcccagaa tgtgcgctgc cgcaccgatg
     4741 acatggccgc tcgacccgac gataagcggg tccggcgcat gaaccagcgc ctcgtccttg
     4801 tctggatacg gcagcatata gagaaaatgc cggagcgctt ccagccgtgc tcgtttcttg
     4861 tcgtccgact tcactacaga ccatggcgca tccgcagtgt cggtatagaa gaacatcgcc
     4921 tccttggctt ccgtgtagtc ttcccatttg tcaatggagg ccagatccat cggcgagagc
     4981 ttccactgct tgagcgggtc gtgctcgcgc gacgcgaacc ttcgacgttg ctcctcgcgg
     5041 gtgaccgaga accagtactt gaagagccag gttcccgtac gaacgagcat gcgctcgaac
     5101 tcggggcact gccgcatgaa ctcgagatag tcatttggct tgcaaaagcc catgacccgc
     5161 tcgacaccgg cgcgattgta ccaggagcgg tcgaagaaca cgatctcgcc gccggctggc
     5221 aggtgctcca cgtagcgctg gaagtaccac tgcgtctgct cccgctcgct cggcttttcc
     5281 agggcgacca cgcgcgcccc gcgcgggttc agatgctcca tgaagcgctt gatcgtgccg
     5341 cccttgcccg ctgcgtcgcg gccttcgaag atgacgacca ccttctggcc cgtcgccttg
     5401 atccaggact gcgccttgag cagctcggcc tgcaactcga gcttgtgcgc ctcgtagctg
     5461 cggcgcgaca tgtggctctt gtagggatac tcaccggttt cgaacagccg tcgaacgacg
     5521 cctggatcac gcttcatctc ggcgagctga tgacgggcga gcgctgcccg gctggaccgc
     5581 ctgccaccgg ccggggtcgg ccccctgccg ttcgccgaga ggtcagcgcc cgccgtctcc
     5641 cggctcacgc cgtcccctgc tgccacgaga tcctgcgccg cttcctttgt cgcatcctgc
     5701 gctgcttctt tcgtcgccat gccgtgccgc cccttgcaga ttgccagctc cgcggcttgc
     5761 ctgacacgga atcgtcaggg ccgcattgag cgggatcaat catggacgac gaagctctac
     5821 ggcatcggat cgacaaccat ggcgatgacc tcgaccagtg tcacctgcac ggtatcaccc
     5881 ggcttcaatg tctcgacaaa ggcacgcatc tccgggtcgc gcaccgccaa tgagcgttcc
     5941 ggcccatcct ggggtctgaa ggtgacgcgg cccgttgccg cgtcgaacgc ctggaaaacg
     6001 acgctggtat cgatcgttgt tacggtagca cccgccggtc tctcgccctc ctcgctgcgc
     6061 atcagggcgg tatcgccctg cgtcgtgatc ggctcggcgc cgggcgggac gaggctcgcg
     6121 acgatgccgc cgtagtagcc gatcgtcacc gtgtcgccga cctcgacctg gggcaggttg
     6181 cgcacctcgg gcggtgccac gacggtgacg aagcgcccgt cctcggtctc gagcagcacc
     6241 tgtcgtgtgg tgaggtccac atcgtgcacg agggcgctgc tgaccgcggt ctgctcgatc
     6301 acggcgccgt cctgggccca actgctcgtg gcagagagga atccggcacc aacaagggcg
     6361 cagacggcga atacaaagcg aagcttggtc atggtgcttt ccctggtttc attcggcagt
     6421 gacagagtag tcgcggtact tgtcgagcag ccggcgcggc ttcttcaacg cgtcgcggcg
     6481 aaacggctca cccagctcct gcgtcaccat gatctccagc accgacggcc ggttgctggc
     6541 gatggcttcc accagcgcat cgccgatctg gtcggggctg tcgaccgtca cgccgttggc
     6601 acccatcgcc cgggcgatgc cggcgaagct cgggttctcg aggttggtgc cgacatagcg
     6661 gtcggcgtag aagtcgatct ggttcttctt ctccgccccc cattgctggt tgttgaacac
     6721 cacggccacc gccgggatgt tctcgcggac cgccgtcagc gtctcctgca ggctcatgcc
     6781 ccaggcgccg tcgccgacat aggcgacggc cggccggtcg ggccgggcga ccttggcgcc
     6841 gatcgccgtc ggataggcgt acccgcaatt gccgaagctc atcgcggcga aaaagctctg
     6901 gggctgctcg aagcgcaggt aggaattcga gaccgagcag atgttgccga tgtcggtcgt
     6961 caccatcgcg ttcttcggca gcgccttctc cagctcgcgc agcgcccgtc gcgggccgac
     7021 cggcgagccg tccgcctggc cccattcgtc gagctcggcc tcccacgccg ccttctgctt
     7081 cccgatctcc gccacccgtg catcgcgatt gccatgcgcc gcgatggccc ggttggcggt
     7141 cgtcagccgc tcgtggatcg cggcagtcgc ggcccgcgca tcgccgcaca ccccgaccgt
     7201 cgcctgtttc accagcccga tccgccgcgg attcgcctcc acctggatca gcttcgcgtt
     7261 cttcggccag tagtcgatgc cgtgctgcgg cagcgtgccg aagggcccga gccgggtgcc
     7321 gagcgccagc accacgtcgg cctgagccag cagcttcatc gccgccttcg agccctggta
     7381 gccgatggga ccacaccaga gcggatgcga ctgcgggaag ctgtcattgt gcaggtaggt
     7441 gttgatgacg ggcgcctgca aatgctcggc gagcctgatc acctcgccca cgccatcgct
     7501 catcaccaca cccccacccg agaggatgac cgggaacttc gcatcggcga tcagcgctgc
     7561 cgcggcatcg aggctttcgg ggccgcccgg gccacgctcg acccggatcg gctcttggat
     7621 cgtgtagtcc gcctcgccgt agaagagatc gcgcgggatg ttgaactggg tcgggccacg
     7681 ttccagcatg gcatagtcga agcagcggcc gagcagctcg gccatccgcc cctggtggcc
     7741 gcaatgcacc tggtacttgg tgatccgctc gaagaagggc atctgctcgg cttcctggaa
     7801 gccgccgaga ccctgcgtgt tcgagcccgt ctcgggggtg atgaccacga ccggtgaatg
     7861 cgcccagtag gcagccgcca ccgaagtcac gaaattggtg atgcccggcc cgttctgggc
     7921 gatgcagacg ccgtgccgcc cggatgcccg ggcatagccg tcggccatgt gcccggcacc
     7981 ctgctcgtgc gccaccgaga cgaagcggat gccggccggc tcgaagatgt cgagcgcgtc
     8041 catgtaggcc gagcccacga tgccgaagac atgagtgacc ccctgcgcca ccagggtctc
     8101 gaccatcgcc tcggacggcg tcatcagcgt catcgtctgc tcccgagttg ccgcaccttc
     8161 aggcgcgaat tctctcattg gccgggtcat agaaggcctc gagccgggcc tcggccggaa
     8221 cctttcgcca gccgatctcg atggcgaact cgcggcccgg cgccaggtcc ttcaccccgc
     8281 ccggcaactg cacatagccc atgccgagcg aggcgtcgat ccggtgacca taggcgcccg
     8341 aagtgatcga gccgacgatc tcgctacccg accagacggg ctcttcctgg tgcaacagct
     8401 cggcgggatc cgccagcttg agctggacga gccgccgcgt tggtccgccg gcctcgcgct
     8461 gccggaaaag cgcctcgcgg ccgatgaaat caggcttgcg ccagccgacg gcaaagccga
     8521 ggcccgcctc gagcggggtg tcctcgacgg agatgtcgtg gccccagtgg cggtacgcct
     8581 tctccatgcg gcaggcgttc atggcgtgat agccggccat gccgagcccg aacgcgctgc
     8641 cggcctcgac gaggacgtcg aagacatgca cggcgaactc cgccggcacg tagagctcga
     8701 agcccagctc gccgacatag gtgaggcggc tggcccggac cacggcacag ccgatctcca
     8761 gctcccgcga ggtggcgaag gggaaatcgg cgtcgcccag gctctcgccc gacaccgcct
     8821 cgagcagggc gcggctctgc ggtcccatga ggcccagcat cggcaggccc gacgtgagat
     8881 cgacgaccgc acagcggcaa ccctccccta catgccggcg cagccaggcg agatcgcggg
     8941 tctggccagc ggccgtcgtg accaccagga actctgtctc gccggtccgg gtcaccgtga
     9001 gatcggcctc gatgccgccc cgctcgttca gccattgggt gtagaccacc cggccgaccg
     9061 gcacgttcat ctcggccgcc gagacatggt tgagtagcgc cagcgcatcc gggccggtca
     9121 cgaggcactt cgtgaagctc gactggtcga agaggacgac agcatcgcgc actgcccggc
     9181 attcggcggc gcacggctcg aaccagttct gccggcccca ggagtagcgg tagtcccgct
     9241 cgccatcggg cgcggcgaac cagttcggtc gctcccaccc ggactgctca cccatcacgg
     9301 cacccagcgc caggaggcga tcatgaaagg gcgagcgccg gacaccgcgg gcagtcgcgt
     9361 attgccggtg cggccagtgc atggcgtaga gcagcccgag cgtctcggtc gtccgctcgc
     9421 gcaggtagcg ctggttggcc tggaacggct gcagccgacg gacatcgaca tcgaccagat
     9481 cggccggcat gtgccggtcc cggatccact cggccagcac cttgccgacc ccgcccgagg
     9541 actggatgcc gatcgagttc atgccgcagg cgacgaagag atcccgaagc tcggccgtct
     9601 cgccgagcag atagcggtcg tcgggggtga agctctccgg gccgttgaag aaggtttgga
     9661 tgccggtctc ggccagcgcc ggcagccgcc tgatcgcagc ctccaggatc ggctcgaaat
     9721 gctcgacatc atcgggcagg gtcgtgaact cgaaatcgtc gggaatgccg cccatgcccc
     9781 agggtttggc gaccggctcg aacgcgccga gcaggagctt gccggcgtcc tccttgacgt
     9841 aggcgcattc gtccggcacc cgcagcaccg gcaggtcccg gggcaggccg gcaatgggct
     9901 cggtgacgat gtagaaatgc tcggcggcat gcagcggcac ggcgacgccg acctgccgcg
     9961 caatggcgtg gctccacatg cccgcggcga gcaccgtggt cgcggcccgg atcggcccct
    10021 cgcgggtcag cacgccggcc gccctgccct gctcgaccag aatccgctcg accgccgtgt
    10081 tctcgaagat tctggcgccg ccggatctgg cccccttggc gtaggccatg gcgacattgg
    10141 cgggatcggc ctggccgtcc ttgggcagga aaatgccgcc cacgagatcg tccgttcgca
    10201 gcagcgggta gcggctcgcg atgtcgccgg gggtcagcac ctcgaccgcg aggccgaaat
    10261 tcttcgccat cgaggcgccg cgcttcagct cctcgaagcg ctcggcatgg gtcgcgacgc
    10321 tgatcgaccc gttctggcga aagccggtcg agaggcccgt ctcggcctcg agcgaccaga
    10381 gcagctcgga agtgtacttg gcgagctcgg tcatccgccg cgaggcgcgc aactggccga
    10441 tcagcccggc cgcgtgccag gtggtgccgg aggtcagccg cttgcgctcg agcacgacca
    10501 cgtcgcgaag cccgagcttg gtcaggtggt aggcgatcga gcagccgatg atgccgccac
    10561 cgatgaccac gacgtcgcag gacgcaggca ggtcgttcgc catttggagc tcgctgacag
    10621 gttcaaggac acccggagct tagcagcgcc gtgcacagga cgacaggcat ggcgcgtgct
    10681 aggatccacg ccggtcaacc gagaagcatg acaggagagg agagaactga tgcgggttgg
    10741 cgtgccgaag gagatcaaga accacgagta ccgcgtgggg ctgacaccgg cgagcgtgat
    10801 cgagtttgtc gctcatgggc acgacgtggt cgtcgagacc ggtgccggca ccgggatcgg
    10861 ctcggacgat gcggcctatg ccgccgctgg cgccgacatc gccgcctccg cggcggacgt
    10921 cttcgcgacc gccgacatga tcgtcaaggt taaggagcca caggcggccg agcgggccat
    10981 gttgcggccg ggccagattc tcttcaccta tctccatctc gccccggacc cggagcagac
    11041 caccgagctc atgcgcagcg gtgccacctg catcgcctac gagacggtca ccgacagcca
    11101 tgggcacctg ccgctcctcg caccgatgtc ccaaatcgcc ggtcgtctcg ccatccaggc
    11161 gggcgcctac tgcctcgagc gggcgcatgg cggctgcggc atcctgctcg gcggcgtgcc
    11221 gggcgtcgag agcgccaggg tgacggtgat cggcggcggc gtggtcggcg agaacgcgat
    11281 catgatggcg ctcgggatgg ccgcggatgt gactgtcctc gaccgcaacg tcgatgcgct
    11341 ccagcacctc gcccgccgct tcgggcccgg cctgaacacc gtctactccc agcgcgcctc
    11401 gctcgaccgc tacgtggtcg acgccgacct cgtgatcggc gccgtcctcg tgcccggcga
    11461 caacgcgccg aagctggtca ccgccgacat ggtcaggagg atgcgcccgg gcacggtgct
    11521 ggtcgacgtc gccatcgacc agggtggctg cttcgagacc tcgcatccga cgacccatgc
    11581 ggagccgacc tacatcgtcg acgacgtcgt ccactactgc gtcgccaaca tgccgggcgc
    11641 cgtgccgcgg acctcgacgc tggcgctcaa caacgtcacc ctgccttatg cgctggccct
    11701 cgccgacaag ggctacaagg ccgccttgcg cgagaacccg catttccgcg ccggcctcaa
    11761 cgtgcatgcc ggcaaggtca cctatcgggc cgtggccgag gcccagggcc acgcttttgc
    11821 cgacccgctg gacgtgctcg ccgaatgagg ccgctcagcc gggaaccagg ccgagtacca
    11881 gggcatagcg cacgcccttg ccgatcccga tgaggatcag ggtcggcagg aagggcgtgc
    11941 ggaagaggcc ggcgacaagg gtcaagggat cgcccacgac ggggacccag gcgaagagca
    12001 gcgaccagat gccccaacgc tgaaaatgcc gggtggcgcg gtccaactgt gccggccgca
    12061 gcggaaagag ccgatggtcg cgccagcgcc gcgcccagcg gccgagggac cagttgagca
    12121 gcgagcccag ggtgttgccc gccgtcgccg tggcccagag cgtcagcttc cctccccaac
    12181 cggccatcgc catgccggcg agcgtgatct cggacgcgaa cgggacaagc gtcgcggcga
    12241 ggaaggccga agcgaaaagc agagcgagct ccgccatgtc agtcggcgcc gaccagcaac
    12301 gcggtcatcg cctcagccgc cgaacgccgc catgacgaga tcgagctgga ggaacagcag
    12361 gaccagcgtt gcattggcgg cccaggcgac cgtcagccag cgggccagcg tgccccagtt
    12421 ggggtccgcg cgaccggcgc agcgccagac ggcaaccagg atccagaccg tgtaggcggc
    12481 gatggccatg agcgagagct gctggaccag ggccagcccc tgccacatca agctggcatg
    12541 gaaaacgatc agcagcaggc tgacgccgac ccccttgccc cagaagagcg ggccgagcgc
    12601 tgcctcgccg cgccaggcac gaatctccga ggcgaacacc tgctcgagca gcgactgcgc
    12661 ctctgacagc cccgcctcgc cttgccggtc ggtgccgttc ggtcctgcgt tcaagggaat
    12721 cccgttcgcc gtctccaggc ccgagctgcg tcgacgtcct cgccatggcg ctcgtggcct
    12781 gcttgcgact gccgaggatg cgccttcggg caggtgtccg gcaagtgtcg gggccgacgc
    12841 tgcgaccatc agaggcaggt cggatcagcg gcaccacgct ggctcggcca agtcagtcca
    12901 gtcgggcgcc agtccgcgga tggaacaacg aggccttgct catgtcgagc gtgaaggctg
    12961 cgcttctgcc gggcacgaca tccgtatccg cgcgcaggcg cgcggtcgcc tcggtcccgg
    13021 cgatgcggac gacggcaagc gtatcggggc cggtgggctc cgtcacctcg acggcgagct
    13081 cgatccccgt ggcgccgtcc gccgcctcgg tgatgctctc cggccgcagg ccgagaaggg
    13141 tggggccgtc gggcgccggc gccatgaggg gcagctcgat gccgccctcg aggtgaacct
    13201 tgctgccgca taccgcaccc ggaatgaggt tcatcgccgg cgagccgatg aaggtggcga
    13261 cgaaggtgtt ggcgggccga tcgtagagct cgcgcggcgt gccgagctgc tgcaggacgc
    13321 ccttgttcat gatggcgatc cgcgtcgcca gggtcatggc ctcgatctgg tcatgggtca
    13381 cgtagacgat ggtggcgccc aggcgctggt gcagcttctt gatctcggtc cgcatctcga
    13441 cccgcagctt ggcgtcgaga ttggagagcg gctcgtcgaa gaggaagatc cggggctgac
    13501 ggacgagggc gcgccccatc gcaacgcgct gccgctggcc gccggagagc tggccgggct
    13561 tgcgggccag cagcggctcg atctgcagga ggcgggcggc atcggccacg gcgacgtcgc
    13621 gctcggcctt cggcaggccg cgcatctcga gcccgaaggc catgttctgg gccacggtca
    13681 tgttggggta aagcgcgtag gactggaaca ccatggcgat gtcgcggtcc ttgggatggc
    13741 gctcgttgac ccgcacaccg tcgatccgga tctcccccgc cgtaacgctt tccagcccgg
    13801 cgatcagcgc gagcagggtg gacttgccgc agcccgaggg gccgaccagc accaggaact
    13861 cgccatcctc gaccgcgatg tcgatcccct cgagcacgcg ggtcgagccg aagcgcttct
    13921 cgacccggtc gatgtcgagc gaagccatgc cgactcagcc tttcacggcg ccggccatca
    13981 ggccgcgcac gaaatagcgt ccggagacga tgtagacgat gagcgtcggc agggccgcga
    14041 ggacggcgcc ggcgaaatgc acgttgtact ccttcacccc ggtcgacgag ctgaccaggt
    14101 tgttgagcgc caccgtcatc ggcgtcgagc cgaagccggc gaacgaggcg ccgaagagga
    14161 agtcgttcca gacattggtg aactgccaga tgacggtgac cgtgatgatc ggcccggagc
    14221 tcggcagcag gatccgccag aagatgcgga agaaccccgc cccgtcgatc tgcgccgcct
    14281 tgaccagctc gctcgggaag gcgtcgtagt agttgcggaa gaagagggtg gtgaagccga
    14341 ggccgtagac gacgtggacg aggacgaggc ccgccgtggt gccggcgagg ccgatgctgc
    14401 cgaggatgcg ggccatcggg atgagcacga tctggaacgg gatgaagcag gaaaagagca
    14461 tcagcgcgaa gaccagccgg tcgcccggaa accgccactt ggtcaggaca tagccgttca
    14521 aggcaccgag cagcgtcgag atcaggacgg ccggcaccac catcttgatc gagttccaga
    14581 aatagggccg aagccccgtc ggctcgacgc cgacctgggc cgtgctccag gcgctggccc
    14641 agggctcggt ggtgaacgcc ccgggcagcg ccagcatgtt gccgccctgg atctcggcga
    14701 gcggtttcac cgagttcacc agcatcacgt agagcggcag gaggaagtag aaggcgaaga
    14761 cgaggagggc ggcgtagagg acggcccgcg ccagcctgtt gcccgcgaag cccccttcgg
    14821 cagccggcgc gctagtggcc gccatgcctg ccctcccgca tctcggcata gagataggga
    14881 acgatcacgg acgcgatcat catcagcatg atgacggccg aggcggcgcc cacgcccatc
    14941 tggttgcggg tgaacgtgta ggaatacatg aacgtcgccg gcatctccgt tgccctcccc
    15001 gggccgccac cggtgagcgc gatcaccagg tcgtaggact tgatggcgag atgcgagagc
    15061 acgacgaagg ccgagaggaa ggccggccgc agctgcggga tgatgatccg ccggtagagc
    15121 tggaaggtcg aggcgccgtc gatctgcgcc gccttcatga tctcgctgtc gatgcccctg
    15181 aggccggcga ggaacatcgc catgacgaag ccggtcgact gccagaccgc cgcgatgacg
    15241 atgcagtaga tcgccatcga gctgtccttg atccagccga aggtgaaatc ggcgaagccc
    15301 cacgagcgga ccacctgctc gagcccgatg ccggggtcga ggaaccactt ccaggcggtg
    15361 ccggtgacga tgaacgagag cgccatgggg tagagataga tcggccggag gaagccctcg
    15421 ccgcggatct tctggtcgag caggatggcg agcagcaggc ccatggccga gcagaagagg
    15481 atgtagagca ggccgaagac ggcgaggttc tggacggcga tggtccagtg ccgcagcccg
    15541 aacaggcgca cgtagttctc gaggccgacc cagccgaagc tcggcaggat gcgcgaatcg
    15601 gtgaaggaga gccagcccgt gaagaagatg aagccgtaga cgaagagcag caccgcggcg
    15661 aagctcggcg ccagcacgag tttcggcaac agctcctgca gccggccgcg cgcgtcagtc
    15721 atgcgcgccg tccgacccgc tggcgcgcga taccgtgaag aacgacgggg ccgccgtcga
    15781 gagggcgacc ccgtgcgaga tcattgcgcc ccttcgaccg ccgcgaccag ctcctcgacc
    15841 gccgtggcgc tgtcgtactc gccgttgaag tgggcggtga cgacgtcgta gacggcattc
    15901 ttgaccgccg cgggcgcgcc atggccatgc gccatcgagc cgtagaggtg gccggccgcg
    15961 gccgcctcgg cgagctccat catgccctgc tggccgcagg cgtcgagctc gtcgccggca
    16021 acgtcggtgc gggccggcac ggagcccttc accttgttga aggcgatctg gaagcccgga
    16081 tcgagaatgg cactcgccag cttcttctgc gcgtcgcggc cttcctcgcc gacctcgaac
    16141 atgacgaact ggtcggcgtt gaaggtcacc gtcccctgcg tgccggggaa gcggaagcag
    16201 aggaaatcct cgcccggcac cttgccggca ttgaggaact cgcccttggc ccagtcgccc
    16261 atgaactgga ccccggcctc cttgttgatg accatggcag tcgcgaggtt ccagtcacgg
    16321 ccggagaagt tgtcgtcgac ataggagcgg atgacggcca tccggtcgaa ggcctccttc
    16381 atcgtgtcgg agccgagcgc ttccggatcg agatcgatca tcgcggcctt gtagaactcg
    16441 ggcccgccgg tcgagacgac gaccgcgtcg aagatggtcg cgtcctgcca ggcctggccg
    16501 ccatgggcca cggccgtgta gccggcggcc tcgatggcat cgagggcggc caccagctcg
    16561 tcccagctcg tcggctgggc gaggccgagc tcgtcgaaga tcgccttgtt ggcccagacc
    16621 cagttggtcg agtggacatt gaccggagcc gccacccact tgccgtcgaa cttcgagaag
    16681 tcctgcaggg ccgccggcac gacggcgtcc cagctctcct cggcggccag ctcattgagg
    16741 tcggcgacga cgccgagcgc ggcccagtcc tggatgtcga agccgagcat ctgcacggcg
    16801 gtcggcgggt tgccgccggt cacccgggcc cggagcacgg tcatcgcctg ctcgccgccg
    16861 ccgcctgcca ccggcatgtc ctgccagccg atgccctcgg cctcgaggtt ctccttcagc
    16921 acgttgaggg ccgccgcctc gccgcccgag gtccaccagt gcagcacctc gacgtcctcg
    16981 gccgtggccc agccggccga ggccatcagc atggtcgttc cggcgagcag tgccctgatc
    17041 tgtttgttca tcgagccgtc cccttcctat cggcggtgcc gggcgattcg cccgttcgcc
    17101 aaattcagtt gacgtcccgt tagagcaccc tcggcccgtg cacaagcgcg gcaatgaggc
    17161 atgcggtccg cggtatgcgg caaggtcgac cggccgctct caggccagcc gcagcagatg
    17221 gtcacgcagg acgaccgcgt cgctgtgctc gacgtcccga gcgccgaaga cgagcgtgac
    17281 ccgccccttg cggcagtgcc cgagcaggtc cttcaccacc tcccggttcg cgtcgagctc
    17341 ctggtcgtag cggctccgga actcgtccca tttctttgga tcgtgcccgt accagcggcg
    17401 caggtccgta ctcggcgcca cctccttcgg ccaatgatcg atcgcgaggt ccgcccggct
    17461 ccgcccgcgt ggccagagcc ggtcgacgag gatccggcag ccgtcctcgg cggttgccgg
    17521 ctcgtaggcg cgcttgagca ggatttccat ggcaggcgcc ggaccctaga cggcgaccca
    17581 gccgcccgac ttctccgagg cgaagagcgc gtccaggacc ttcatgttgg cgatcgattc
    17641 gtcgagcggc atggcgagtt cgccctcgcc acgcacggcc ttcgagaaca tctcgccctg
    17701 caggcggtac tggtcgcaga ttggcaggtc gatcacctcg cggccgccac cgacgagatc
    17761 ccggccatcg tcgacggtga tccggcacgg ccggtcgttc ggggcgttgc aggggatctc
    17821 gacctcgagc cggcccttgg tgccgagcac ggtgaagcgc tgcgccggcg cgagctgggt
    17881 cgaggtggtc cagaccgaac ggcgcccgtc gccgaagtcg aggatcgccg aggccagccg
    17941 gtcggtgccg aagcccgggt cgcgctcgat gaggccgacc gcgcgcttgg gctcggcccc
    18001 cagcatccag cgcgagaaga tcgtcatgta gcagccgata tccatgatcc cgccaccgcc
    18061 gatatc
//
LOCUS       DAOTFI010000090        18041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3738_length_18041_cov_7.016513, whole genome shotgun sequence.
ACCESSION   DAOTFI010000090 DAOTFI010000000
VERSION     DAOTFI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 18041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18041
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3738_length_18041_cov_7.016513"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="queG"
                     /locus_tag="P5558_10830"
     CDS             complement(<1..823)
                     /gene="queG"
                     /locus_tag="P5558_10830"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRY24847.1"
                     /translation="MTTTSISETLRERASSLGFADLRITPAEVPKAAEAAYRDFIAGG
                     RHGTMDWLAREPDRRAQPAGMWPEARTAIMLAMNYGPAHDPLAVLARKDRAAISVYAQ
                     NRDYHDVMKGRLKELAGWLHRETGAEVKVFVDTAPLMEKPLAQQAGLGWQGKHTNLVS
                     RRFGSWLFLGSILTTAELPVDAPETDHCGACRRCLDACPTGAFPRAYELDARRCLSYL
                     TIEHKGSIPHEFREPMGNRIYGCDDCLAVCPWNKFAVEAQEAKLQARDELVAPSLE"
     gene            complement(801..1466)
                     /locus_tag="P5558_10835"
     CDS             complement(801..1466)
                     /locus_tag="P5558_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY24848.1"
                     /translation="MLRLYHQPLCPFSRKIRLVLREKNLAAELVEVEPWRREPSFLEL
                     NAAAEVPVLVDGEAVVADSAAICDHLHETHPEPDLLGQTQAQRDETRRLVGWFDVKFR
                     REVTDLLWGEKLLRRLKRNGTPSSEALRAGAANIRGHLAYIGYLYEDRRWLAGDAMTM
                     ADLTAAAHISVLDYLGDVPWEASSGARDWYAKVKSRPAFRPLLMDRITSIKPPVHYDD
                     LDF"
     gene            complement(1579..2280)
                     /locus_tag="P5558_10840"
     CDS             complement(1579..2280)
                     /locus_tag="P5558_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRY24849.1"
                     /translation="MITIRKAAERGHADFGWLDSRHSFSFGQYHDPRHMGFGPLRVIN
                     DDRVAAGRGFGTHPHRDMEIVTYVLEGALEHQDSLGSGSVMRPGDVQRMSAGTGIRHS
                     ELNHSATEPVHFLQIWIEPERTGLAPSYAQAHVDPADRQGRLVLVASRDGRAGSLSLN
                     RDADIHVARLAPGEAVGHTPEAGRMLWLQVASGSVRLNGQELAEGDGAALTGEPALEI
                     EGVEAAEILLFDMAG"
     gene            2406..3095
                     /locus_tag="P5558_10845"
     CDS             2406..3095
                     /locus_tag="P5558_10845"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRY24850.1"
                     /translation="MRMTWLGHSAVHLETAGKSVLIDPFLTGNPKHPEGWEDGLERLD
                     AIVVTHGHEDHTGDTVRLAKKYGATLFAQYEICMWMNGQGVEKVQPMNTGGTVEEGGL
                     AFSMVPAFHSAGIMRDGEPRSMGDPAGFVVTGSEGAVYHAGDTCLFSDMKLIQRLYRP
                     KVGFLPVGDRFTMGPAHAAIACNEFLELELVVPVHWGTFDLLTGDPEVLRKEVRRGRV
                     HIAEPGVPFGP"
     gene            complement(3138..3656)
                     /locus_tag="P5558_10850"
     CDS             complement(3138..3656)
                     /locus_tag="P5558_10850"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY24851.1"
                     /translation="MAIGRAVRVGLDGLYRLCGLVAALFLVALLLIILAQIGARWLGV
                     QFPGSTSYAGYCMAGASFFALAYTLNHGAHIRVSLFIGQLTGAPRRAAELWCLAIAAG
                     LAWYFAWYAIKAVRISRLIHDISQGQDATPLWIPQSFMAMGTVVLAIAFTDHLVRVLR
                     GGELPGEEAAQE"
     gene            complement(3679..4653)
                     /locus_tag="P5558_10855"
     CDS             complement(3679..4653)
                     /locus_tag="P5558_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY24852.1"
                     /translation="MKKLNALAGAAALALGAGAAGAAETWDMPMAYPDSNFHTENGKA
                     FAACVGERSNGELEIVVHGGGSLFKGDEIKRAVQTGQALIGERLLSAHANENPLFGVD
                     SIPFLATSYEASDKLWQASRPALEELLASQNMVLLYAVPWPPQGLYTKKELNAASDME
                     GVKFRSYNAATARMAELMGAVPVQIEAAELSQALATGVAESFISSGSTGYDSKVWEQL
                     THWYEVNAWLPRNHVLVNQQAWEGLDDATRTALSECAAEAEAQGTAKSEELNDWYKEQ
                     LAANGMTVTPPGDQLRADLEAIGAQMTEEWLASAGESGQAVIEAYRGM"
     gene            4858..5499
                     /locus_tag="P5558_10860"
     CDS             4858..5499
                     /locus_tag="P5558_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24853.1"
                     /translation="MRSSLIPIGLASVVWTVVIGAGAALTADPFRPDGHFQMPDPAVL
                     DPGMAEHLYGRRVDGMAKNYALSGVPAARLYRSWQRANRAPYRSATHGDRYVNNYVNG
                     IAAERYGRLAEGEAMPVGSIIAKDAIAVDRQLRVFDGPLSIMEKMPEGYDPEARDWRY
                     VLILPDGSIFADSSGLNQEGTDFCAACHQGAGDAADHLFFIPDPYSRAFRPQG"
     gene            complement(5456..6856)
                     /locus_tag="P5558_10865"
     CDS             complement(5456..6856)
                     /locus_tag="P5558_10865"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY24854.1"
                     /translation="MARRGWLDRLRGATDDSSAALIRATLESHEERNLKRLTIARSLM
                     FLAIFFWLGFNYGWSTAADNAVILLAFIANGLVAYALARGRSDRRWVPLAVTLVDAIL
                     LGFTLLAPGRTYPGDWPWATVLRQPSFLYFLLLPAIASLSFRPIAVLWSAFCVGLVWA
                     LGTMLIVHSPGTSTTIPNYGEAGPEASASQLAAYLSPHYVHVDDAVVRIFLTFMIAAI
                     LAFGAYRARRLMLEQAEVVRQRANLARYVAPNMVEALARTDRPMGEVRTHSAAVLFAD
                     IKGFTALAEAGSPEETMRLLREFHGRMANCVFALGGTFDKFIGDGLMATFGTPEPAAD
                     DADRALACGRAMLAAIADWRQTRTALGEAPVEVGIGIHFGPVVMGDIGGGEADQRFEF
                     AVIGDTVNVASRLERLTRSVDRPLLVSEALCRALQAPADDLEPLGPHHLDGRAGQVEV
                     WAVNPADERPGCRDRG"
     gene            complement(6882..7232)
                     /locus_tag="P5558_10870"
     CDS             complement(6882..7232)
                     /locus_tag="P5558_10870"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRY24855.1"
                     /translation="MKVDLTTLPVVRQPPDERQFWRKLTRVLAAIPFAEDLVAAWFCA
                     RDPATPGYVRAVLFGAIAYFLMPVDLVADYLVGVGFTDDAAVIAMVLSTLGSNIAPAH
                     REAARRRLADLRGA"
     gene            7278..7754
                     /locus_tag="P5558_10875"
     CDS             7278..7754
                     /locus_tag="P5558_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY24856.1"
                     /translation="METPTPPEGPRIDEAEIEDLEQIVAMLADDVLGATREIPPPPLD
                     PCYVRAFEAIDADPLHSLLVLREGDRVIGCLQLRLMPSLSRRGMLRALIENVRVAKEA
                     RGRGLGEFLLQAAIERARAAGCGLVQLTTDRQRLDAHRFYERLGFAATHLGMKLNL"
     gene            7778..>8123
                     /locus_tag="P5558_10880"
     CDS             7778..>8123
                     /locus_tag="P5558_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRY24857.1"
                     /translation="MADTAERLSTLDEFLAFDGEGDVRHQLVRGVITAMAPAQVVHGR
                     LVARLARAMGNQLRAPCEPITEAGIKPAQRDDTYWQADLVVHCRPLVPGQIYLTDPRL
                     VVEVLSASTEATD"
     assembly_gap    8124..8223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8224..8511
                     /locus_tag="P5558_10885"
     CDS             <8224..8511
                     /locus_tag="P5558_10885"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRY24858.1"
                     /translation="TDPRLVVEVLSASTEATDRLLKLDDYRLIESVHDILLVATERVR
                     IEHWQRREGFWAVRDLGPGDVLEVAELGLEVALDPLYADLPEAEGEGGEAA"
     gene            complement(8463..9788)
                     /gene="eno"
                     /locus_tag="P5558_10890"
     CDS             complement(8463..9788)
                     /gene="eno"
                     /locus_tag="P5558_10890"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRY24859.1"
                     /translation="MTRATSIEHVHGRRVFDSRGRPTVEAEVRLQGGAVGRAIAPAGA
                     SKGSAEAVDRRDGGSVMGGHDVRGAVAAVGERIAPALRGLNGRDQVAVDEALIALDGT
                     ANRSGLGANAMIAVSMAVAWAAAEAAGQPLWRYLLGDRQGPVTLPLPEIQIFGGGAHA
                     ARRVDIQDFMIVCPGAETVAQALDWTAEVYRAAGELMAESGRLAGVADEGGWWPVFAS
                     NEEALETLVQAIERAGRRPGDEVAISLDVAASEFGRDGSYRLARDGRELGRDAMIELL
                     EGWLARYPIVSIEDPLAEDDAEGFLRFTRRFGHAVQIIGDDFLVTDAARVRAAAQTGI
                     ANAVLIKPNQRGTLMETLECWKAAKAYGFGGIVSARSGETEDVTICHLAVGWQVPQLK
                     VGSFSRSERMAKWNELLRLEEALGDRARFAGWAPYAASPPSPSASGRSA"
     gene            complement(9785..10792)
                     /locus_tag="P5558_10895"
     CDS             complement(9785..10792)
                     /locus_tag="P5558_10895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRY24860.1"
                     /translation="MRVGWNSDIMAVLRRAGLLELGAPPPMVPLTGGVSSDIWRVDIA
                     GKQYCVKRALAKLKVQADWRAPIERNSYEAAYLEVVDRLVPGAVPGLIHVDAAASAIV
                     TRYLDPKAYELWKTALMAGQIDPAVAAAVGDRLGRIHSLTANDADIAARFRSDQVFHA
                     IRLEPYLLATAETQPMVASQLRALARRTAATRICLVHGDVSPKNILVGRLGPVLLDAE
                     CAWYGDPAFDLAFVLNHLALKARHRPTDARRFAEAFRRLVEAYLGHVDWEPVAAVEER
                     ATSLLPGLMLARVDGKSPVEYLTSGREKADVRAFAIHHLTEPVARLEAFREAWFTGFG
                     Q"
     gene            10865..11800
                     /locus_tag="P5558_10900"
     CDS             10865..11800
                     /locus_tag="P5558_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24861.1"
                     /translation="MQRVLFHYQAGERLTRRLRALAADGLDVRIVAPEDADGLARELS
                     GTEILWHALAPVTAELIEAAPRLRLIQKWGVGLNTIDLAAAQARGIQVANMPGTNTRA
                     VVELTLLLILACLRRLAEVDPALRGGQWAPPLATMEALGELGGRRVGLVGMGAVPQML
                     APILKAMTAEPVYWSRAEKPDAPARFLPLAELLATSDVVSLHLPLTEETTGLVDVRAI
                     KPGAILVNTARGGLVDEPALVGALRSGRLGAAGLDVFEAEPIDPNNPLLALPNVTVTP
                     HLAWLTGETLERSLEVARQNALNLRTGEPLLFRVA"
     gene            11918..>13977
                     /locus_tag="P5558_10905"
     CDS             11918..>13977
                     /locus_tag="P5558_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY24862.1"
                     /translation="MTSTIHATVCPHDCPSVCALEVEKTAEGRLGKVKGSKRNSYTAG
                     VVCAKVSRYAERYHHKDRLAFPMRRTGERGSTDFARIGWDEALDEIALAFTRAEQRHG
                     SETVWPYWYAGTMGFVQRDGVQRLTHARRWSRFKSTICTTLADSGWRAGYGKRWGVPA
                     EEAGDHADLIGVWGCNPVATHVNLMSHVARARKRGALFFVVDPYRSGTAEQADLHLPV
                     RPGTDGALACAVMHILFRDGHADREYLARMTDFPAELEAHLALRGPDWACAITGLDVA
                     LIERFAALYGNAARPWIRFGFGFTRSRNGSAAMHAASCLPVVKGAWQHEGGGGLYNMG
                     DLYHWDKTTIEGHDVMDPATRLLDQSRIGPVLTHDPLDLQGGPPVTALLVQNTNPMCI
                     APDLGKVHQGFARPDLFVAVQEQFMTETAKQADILLPATMFLEHDDIYHASGHSRFQI
                     GRKIFEPHADVRSNHFVVCELAKRLGAEHRGFHMTEWELIEDLLARSGWPDAATVHES
                     GGWEALPDADTAHYRNGFPTADGRFHFRADWALQGADHARMPALPDHLAITDEITEEH
                     PFRLVAAPARQFLNTSFTELDSGRKREGRPTAMIHPDSLDRLGIAEGDLVALGNARGS
                     VRLHAVARDGQHPGTIVVESIWPNPHWVDGIGINLLLGADAAPPAGGAAIHDTAVWLR
                     PAAG"
     assembly_gap    13978..14077
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14085..14576)
                     /locus_tag="P5558_10910"
     CDS             complement(14085..14576)
                     /locus_tag="P5558_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24863.1"
                     /translation="MRGALPLPTALLLPILLFGVGCAGKTEEAAVCPDSAIIYGLDRF
                     NGTDANGQKIVANIENIDGLCQLQGETLDLDISFDIVVDGPEGTPITYFVVLADPAGN
                     VIEKASFATAVPAGAGDGSVRLREQLEQRVQNVAAGTSADYSILIGFDLPPETALEQR
                     RAL"
     gene            complement(14588..14839)
                     /locus_tag="P5558_10915"
     CDS             complement(14588..14839)
                     /locus_tag="P5558_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRY24864.1"
                     /translation="MSETATLPLMPQKAPFKVEVTAGKAYWWCACGQSRSQPFCDGSH
                     KGSDFTPVKHTAARDGALWFCGCKRSGKQPLCDGTHKAL"
     gene            14992..16044
                     /locus_tag="P5558_10920"
     CDS             14992..16044
                     /locus_tag="P5558_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRY24865.1"
                     /translation="MYRDADQTTPPNGGARGVGRPWRSPRAGPPTRIAALDLGTNNCR
                     LLVAEPHDSTFDVVDSFSRITRLGEGLALSGGLGEPAIRRTLAALAVCKRIIDRNRVA
                     ELRCVATEACRRANNGAAFIAQVEREIGLRLEVLSQDEEARLALLGCLPLIDEAADEL
                     LMVDIGGGSTEVIWMDRAGGGPGEAVSLPLGVVTLAESFGREPDESEYAAMVRTARHA
                     LAEVALGHFVPRSLDPPAVQMLGTSGTVTTLAAVHLGLRRYDRRRVDGLTLPYAAIAA
                     VTERLRRLDHQGRAAHPCIGQGRADLVIAGCAILDAIHDCWPISRLRVADRGVREGIL
                     NSILGLDLGAALRPVA"
     gene            16062..16733
                     /locus_tag="P5558_10925"
     CDS             16062..16733
                     /locus_tag="P5558_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRY24866.1"
                     /translation="MQRTEKTTLKRSRTRRASSTRWLQRQLNDPFVQQAQRAGWRARS
                     VFKLEEIDTRFKLIAKGEAVVDLGAAPGSWSQYAAAKGARVLALDLLPVDPINGVEII
                     QGDFLEPGIQARLLERLGSKVKTVLSDIAPDATGRRLVDRLRAEAVGEAVIDFATLVL
                     APEGQVLIKLVKGAETRVLDRARPHFAKLRLIRPKATRSDSSETYLLASGFRPTEPEV
                     DQPGT"
     gene            complement(16718..17611)
                     /locus_tag="P5558_10930"
     CDS             complement(16718..17611)
                     /locus_tag="P5558_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24867.1"
                     /translation="MGKRIVFTGGSGKAGRHVVPYLLERGHAVLNLDLVPLDRPGVST
                     VIADLTDSGQAFDALSMHFGFAGFATGKGPAPVDAVVHFAAVPRVLIRPDNETFRINV
                     ISTYNVIEAAVKLGIRKIVIASSETTYGVCFAEGDKDFHAFPLEEDYDVDPMDSYGLS
                     KVVNEKTGRAFAMRTGADIYALRIGNVIEPHEYDRFAAFLQDPPSRKRNAWSYIDARD
                     LGQIVDRCLARDGLGFQVFNAVNDTITAREPTEAFLREHAPGTPITRPLGDFEAPISN
                     RKIRDVLGFEEEHDWRRYVPG"
     gene            complement(17647..>18041)
                     /locus_tag="P5558_10935"
     CDS             complement(17647..>18041)
                     /locus_tag="P5558_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24868.1"
                     /translation="LVRLDRSARVPDAPVRLHREPVGRPVVGFDAERRVVLAASLADG
                     RLLVLPIDGDGRPGDAELCEGRFAEALAIMADETAVPLLVAIDREEQILIRPLAERGS
                     AFERLGSLEDVLEGLPGEPPRGRPRVAA"
BASE COUNT         2644 a         6371 c         6228 g         2598 t
ORIGIN      
        1 gctccaggga aggtgccacc agctcgtcgc gcgcctgcag cttcgcctcc tgcgcttcga
       61 ccgcgaactt gttccaggga cagacggcga ggcagtcgtc gcagccatag atgcggttgc
      121 ccatgggctc gcggaactcg tgcgggatcg accccttgtg ctcgatcgtc agatacgaga
      181 ggcagcgccg ggcatcgagc tcgtaggccc tggggaaggc cccggtcggg caggcatcga
      241 ggcagcggcg acaggcgccg caatggtcgg tctccggcgc gtcgaccggc agctcggccg
      301 tggtcaggat cgagcccagg aagagccacg agccgaagcg gcgcgagacg agattggtgt
      361 gcttgccctg ccagccgagc cccgcctgct gcgccagggg cttctccatg aggggtgcgg
      421 tgtcgacgaa gaccttgacc tcggcaccgg tctcgcggtg cagccagccg gccagctcct
      481 tgagccggcc cttcatcacg tcgtggtagt cgcggttctg cgcatagacc gagatggcgg
      541 cccggtcctt tcgggccagc acggccaggg gatcgtgggc ggggccgtag ttcatggcga
      601 gcatgatggc ggtccgcgcc tctggccaca tgcctgccgg ctgggcccgg cggtcgggct
      661 cgcgggcgag ccagtccatc gtgccgtgcc ggcccccggc gatgaaatcg cgataggccg
      721 cctcggccgc cttcggcacc tcggctggcg tgatcctgag atcggcgaag ccgagtgagg
      781 aggcgcgttc cctgagcgtt tcagaaatcg aggtcgtcgt aatgcaccgg tggcttgatg
      841 ctggtgatcc ggtccatgag cagcggccgg aaggccggcc gggacttgac cttggcgtac
      901 cagtcgcgcg cgccggacga cgcttcccag ggcacgtcgc cgagatagtc gaggacggag
      961 atgtgcgcgg cggcggtgag atcggccatc gtcatggcat cgccggcgag ccagcggcgg
     1021 tcctcgtaga gatagccgat ataggcgaga tggccgcgga tgttggcggc acccgcgcgg
     1081 agcgcctccg agctcggcgt gccgttgcgc ttcagccggc gcagcagctt ctcgccccag
     1141 agcaggtcgg tgacctcgcg gcggaacttg acgtcgaacc agccgaccag ccgccgcgtc
     1201 tcatcgcgct gggcctgggt ctggcccaaa agatcgggct cgggatgggt ctcgtgcagg
     1261 tggtcgcaga tggcggcgct gtcggccacc accgcctcgc cgtcgaccag gaccggcacc
     1321 tcggccgcgg cgttgagctc caggaacgac ggctcgcgcc gccagggctc gacctcgacc
     1381 agctcggccg cgagattctt ctcgcgcagc accagccgga tcttgcgcga gaagggacag
     1441 agcggctggt gatagaggcg gagcatcggc gccttggcga tctcgtgcgg aagcctgatg
     1501 tattgatgaa agcgcagcgg cgccggcagc gcaaggctgc tgcacggcgc cggcccgcgg
     1561 gcatggtgct tgccggcgtc agccggccat gtcgaagagc aggatctcgg ccgcctcgac
     1621 gccctcgatc tcgagcgccg gctcgcccgt cagggccgcg ccgtcgccct cggcgagctc
     1681 ctggccgttg agccggacgc tgccgctggc cacctgcagc cagagcatcc ggccggcctc
     1741 gggcgtgtgg ccgaccgcct cgcccggggc gagacgggca acatggatgt cggcgtcccg
     1801 gttcaggctg agcgagcccg cacggccatc tcgcgaggcg accaggacga gccggccctg
     1861 ccggtcggcg gggtcgacat gcgcctgggc gtaggacggt gccagcccgg tgcgctccgg
     1921 ctcgatccag atctgcagga aatgcacggg ctcggtggcc gagtggttga gctcggagtg
     1981 acggatgccg gtgccagcac tcatccgctg cacgtcaccc ggccgcatga ccgagcccga
     2041 gccgaggctg tcctggtgct cgagcgcccc ctccaggacg taggtcacga tctccatgtc
     2101 gcgatgcggg tgggtgccga agccgcgccc ggcggccacc cggtcgtcgt tgatcacgcg
     2161 cagcgggccg aagcccatgt ggcgcgggtc gtgatactgg ccgaagctga agctgtgccg
     2221 gctgtcgagc cagccgaaat cggcatggcc gcgctcggcg gccttgcgga tcgtgatcat
     2281 ggccttgtct ccatggctgt ctgccgccta tctggttggt cgtcgtggcc cgcaccacgg
     2341 gcccggcgga attccaccgt tgccggcgag gcaacgatcg aaaccggacc ggaggagacg
     2401 acctcatgcg catgacctgg ctcggccact cggccgtcca tctcgagacg gcgggcaagt
     2461 cggtgctgat cgatcccttc ctcaccggca atccgaagca ccccgagggc tgggaggacg
     2521 gtctcgagcg cctggacgcc atcgtcgtca cccacggcca cgaggaccat acgggcgaca
     2581 ccgtgcggct ggcgaagaag tacggcgcca cgctcttcgc ccagtacgag atctgcatgt
     2641 ggatgaacgg gcagggtgtc gagaaggtcc agccgatgaa caccggcggc acggtcgagg
     2701 agggcggcct cgccttctcg atggtcccgg ccttccacag cgccggcatc atgcgcgacg
     2761 gcgagccccg gtcgatgggc gacccggcgg gcttcgtcgt caccggcagc gaaggggcag
     2821 tttaccacgc gggcgacacc tgcctcttct ccgacatgaa gctgatccag cggctctatc
     2881 ggccgaaggt cggcttcctg ccggtcggcg accgcttcac catgggtccg gcgcatgccg
     2941 ccatcgcctg caacgagttc ctcgagctcg agctcgtcgt gccggtccac tggggcacgt
     3001 tcgacctgct caccggcgat ccagaggtgc tccgcaagga ggtgcggcgc ggccgggtcc
     3061 acatcgccga gccgggcgtg ccgttcggtc cttgatcggg cggcgcaggc tcagggcagg
     3121 ccccgcctgg cctcccccta ctcctgcgcc gcctcctcgc ccggcagctc gccgccgcgc
     3181 agcacgcgga cgaggtggtc ggtgaaggcg atggcgagca ccaccgtgcc catcgccatg
     3241 aagctctgcg ggatccagag cggcgtcgcg tcctgtccct ggctgatgtc gtggatcagc
     3301 cgcgagatgc ggacggcctt gatggcgtac caggcaaagt accaggcgag cccggcggcg
     3361 atggcgaggc accagagctc ggcggcgcgg cgcggtgccc cggtgagctg gccgatgaag
     3421 agcgagaccc ggatatgggc gccgtggttc agggtgtagg cgagggcgaa gaacgaggcg
     3481 ccggccatgc agtagccggc atagctggtg gagcccggga actgcacgcc cagccagcgg
     3541 gcgccgatct gcgccaggat gatgagcagg agggcgacga ggaagagggc ggcgacgagg
     3601 ccgcagagcc ggtagagccc gtcgagcccg acccgaaccg cgcggccgat cgccacccgc
     3661 ttccccgacc cccccggctc acatgccgcg ataggcctcg atcaccgcct ggccgctctc
     3721 gccggcgctg gccagccact cctcggtcat ctgcgcgccg atcgcctcga ggtcggcgcg
     3781 gagctggtcg ccgggcgggg tcacggtcat gccgttggcg gcgagctgct ccttgtacca
     3841 gtcgttcagc tcctcggact tcgccgtgcc ttgcgcctcg gcctcggcgg cgcactcgct
     3901 gagtgccgtg cgggtggcgt cgtcgaggcc ctcccaggcc tgctggttga ccaggacgtg
     3961 gttgcggggc aaccaggcat tcacctcata ccaatgggtg agctgctccc agaccttgct
     4021 gtcgtagccg gtcgagcccg aggagatgaa gctctcggcc acgccggtcg ccagcgcctg
     4081 gctcagctcc gccgcctcga tctgcactgg cacggccccc atcagctccg ccatgcgcgc
     4141 cgtcgcggcg ttgtaggagc ggaacttcac gccctccatg tcggaggcgg cgttcagctc
     4201 cttcttcgtg tagaggccct gcggcggcca tggcacggca tagagcagga ccatgttctg
     4261 gctggcgagc agctcctcca gcgccggccg gctggcctgc cagagcttgt cggaggcctc
     4321 gtagctcgtc gccaggaacg gaatgctgtc gacgccgaag agcgggttct cgttggcgtg
     4381 cgccgagagc agccgctcgc cgatcagcgc ctggccggtc tgcaccgcgc gcttgatctc
     4441 gtcgcccttg aagagcgagc cgccgccatg gacgacgatc tccagctcgc cattgctccg
     4501 ctcgccgacg caggcggcga aagccttgcc gttctcggtg tggaaattgc tgtcgggata
     4561 ggccatcggc atgtcccagg tctccgccgc gccggcggcc ccggcgccca gcgccagcgc
     4621 cgcggcaccg gccagcgcgt tcagcttctt catggctgct ctcctcctga tgtgaccggc
     4681 ccgacgcacg cgccgggcga acgcctcgct cggatgaaac cctagtcatc ggaggccgtg
     4741 ccggtcaacg acggcggcgc cgcacgccgc cgtgactttg agcccggggg gcgatggcgt
     4801 atccttccac ctgcgcgatc gctcggagag cccggaaata cggctgagga ggtcggcatg
     4861 cgctcttccc tgatcccgat cggtctcgcg tcagtggtgt ggacggtcgt cattggcgcg
     4921 ggcgcggcgc tgaccgcgga tccgttcagg cccgacgggc atttccagat gcccgatccg
     4981 gccgtgctcg atccgggcat ggccgagcat ctctatggcc ggcgggtcga cgggatggcc
     5041 aagaactatg ccctgtcggg cgtgcccgcc gcccgtctct accgctcctg gcagcgcgcc
     5101 aaccgggcac cctaccgttc cgcgacccac ggcgaccgct acgtcaacaa ctacgtcaac
     5161 gggatcgcgg cggagcgata tggccgcctg gcagagggcg aggccatgcc ggtcggcagc
     5221 atcatcgcca aggacgcgat cgccgtcgac cggcagttgc gcgtcttcga cggcccgctc
     5281 agcatcatgg agaagatgcc cgaaggctac gaccccgagg cgcgcgactg gcgctacgtc
     5341 ctgatcctgc ccgacggcag catcttcgcc gacagcagcg gcctcaatca ggaaggcacc
     5401 gacttctgcg ccgcctgcca ccagggcgcc ggcgacgccg ccgaccacct gttcttcatc
     5461 cccgatccct acagccgggc ctttcgtccg cagggttgac cgcccagacc tccacctggc
     5521 cggcgcgccc gtccagatga tgcggcccga gcggctcgag gtcgtcggcc ggtgcctgca
     5581 gcgcccggca gagggcctcg ctgaccagga gcggtcgatc gacgctgcgg gtcaaccgct
     5641 cgaggcggct cgccacgttg acggtgtcgc cgatcacggc gaactcgaag cgctggtcgg
     5701 cttcgccgcc accgatgtcg cccatgacga ccggcccgaa atggatgccg atgccgacct
     5761 cgaccggcgc ctcgcccagc gccgtccgcg tctggcgcca gtcggcgatg gcggcgagca
     5821 tggcccggcc gcaggcgagc gcacggtcgg catcatccgc tgccggctcc ggcgtgccga
     5881 aggtggccat cagcccgtcg ccgatgaact tgtcgaaggt gccgccgagg gcgaagacgc
     5941 agttcgccat ccggccgtgg aactcgcgca gcagccgcat cgtctcttcc gggctgccgg
     6001 cctcggcgag tgcggtgaag cccttgatgt cggcgaagag cacggccgcg ctgtgggtgc
     6061 gaacctcgcc catcggccgg tcggtccgcg ccagggcctc caccatgttc ggggcgacgt
     6121 agcgggcgag attcgcccgc tgccgaacca cctcggcctg ctcgagcatc agccggcggg
     6181 cgcgataggc gccgaaggcg agaatggcgg cgatcatgaa ggtcaggaag attcgcacca
     6241 ccgcatcgtc gacatgcacg tagtggggcg agaggtaggc cgcgagctgg ctggcgctcg
     6301 cttccgggcc cgcctcgccg tagttgggaa tggtcgtcga cgtgcccggc gaatgcacga
     6361 tcagcatggt gccgagggcc cagacgaggc ctacgcagaa cgccgaccag agcaccgcga
     6421 tcggccggaa gctcaggctg gcgatcgccg gcagcaggag gaagtagagg aagctcggct
     6481 ggcgcaggac ggtggcccaa ggccagtcgc cgggataggt gcggccgggt gccagcaggg
     6541 tgaagccgag gaggatcgca tcgaccagcg tcacggcgag cggcacccag cgccggtcgc
     6601 tgcggccgcg tgccagggcg taggcgacca ggccattggc gatgaaggcg aggaggatca
     6661 ccgcgttgtc ggcggcggtc gaccagccgt agttgaagcc gagccagaag aagatggcga
     6721 ggaacatgag gctgcgggcg atcgtcagcc tcttgaggtt gcgctcctcg tggctctcga
     6781 gggttgccct gatcagggcc gcggacgagt cgtcggtcgc gccgcgcagc ctgtcgagcc
     6841 agccgcgacg cgccatctga gaagccctcc cggccggtcg ttcaggcgcc gcgcaagtcg
     6901 gccagtcgtc gccgcgccgc ctcgcgatgc gcgggcgcga tgttgctgcc gagggtcgag
     6961 aggaccatgg cgatcacggc ggcgtcgtcg gtgaagccga cgccgaccag atagtcggcg
     7021 accaggtcca ccggcatcag gaaataggcg atcgccccga acagcaccgc ccgcacatag
     7081 ccgggcgtcg ccggatcgcg ggcgcagaac caggccgcga ccagatcctc ggcgaacgga
     7141 atggcggcca gcacccgcgt cagcttgcgc cagaactggc gctcgtcggg cggttgccgg
     7201 accacgggca gggtggtgag atcgaccttc atgggcctaa gatggcgagc cctgagcaca
     7261 ggatcaagcg agcgccgatg gaaaccccga caccgccaga ggggccccgg atcgacgagg
     7321 ccgagatcga ggatctggag cagatcgtgg ccatgctcgc cgacgacgtg ctcggcgcca
     7381 cgcgcgagat cccgccgccg ccgctcgacc cctgctatgt ccgggcattc gaggcgatcg
     7441 acgccgatcc gctgcacagc ctcctggtcc tgcgcgaggg cgatcgggtg atcggctgcc
     7501 tgcagcttcg cctcatgccg tcgctcagcc ggcgcggcat gctgcgcgcc ctgatcgaga
     7561 atgtccgggt ggcgaaggag gcgcgcggcc ggggcctcgg cgaattcctg ctgcaggcgg
     7621 cgatcgagcg cgcccgggcc gccggctgcg gcctcgtgca gctcaccacc gatcgccagc
     7681 gcctggacgc gcaccgcttc tacgagaggc tcggcttcgc ggcgacccat ctcggcatga
     7741 agctgaatct ctgagcgttt tctgctagga tcgggccatg gccgatactg ccgagcgcct
     7801 ttcgaccctg gacgagttcc tcgccttcga cggcgagggc gacgtgcgcc atcagctcgt
     7861 ccgcggcgtg atcaccgcca tggcgccggc gcaagtggtt catgggcggc tggtggcacg
     7921 ccttgcgcgg gccatgggca accagctccg tgcgccgtgc gagccgatca ccgaggcggg
     7981 catcaagccg gcccaacgcg acgacacgta ctggcaggcc gatctcgtcg tgcactgtcg
     8041 gcccctcgtc ccggggcaga tctatctcac cgacccccgg ctcgtggtcg aggtcctctc
     8101 ggcctcgacc gaggccaccg atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnaccgacc cccggctcgt ggtcgaggtc ctctcggcct cgaccgaggc caccgatcgc
     8281 ctcttgaagc tcgacgacta ccgcctgatc gagagcgtgc acgacatcct gctggtcgcc
     8341 accgagcggg tgcgcatcga gcactggcag cgccgcgagg gcttctgggc ggtccgcgat
     8401 ctgggccccg gcgacgtgct ggaagtcgcg gagctgggcc tggaggtggc gctcgatccg
     8461 ctctacgcgg atctgcccga ggccgaaggc gagggcggcg aggccgcgta gggcgcccac
     8521 ccggcgaagc gcgcgcggtc gccgagcgcc tcctcgagcc gcagcagctc gttccatttc
     8581 gccatccgct cggagcggct gaacgagccc accttgagct gcggcacctg ccagccgacc
     8641 gccagatggc agatggtgac gtcctcggtc tcgcccgacc gcgccgagac gatgccgccg
     8701 aagccgtagg ccttggcggc cttccagcat tcgagcgtct ccatcagcgt gccgcgctgg
     8761 ttgggcttga tcagcaccgc gttggcgatc cccgtctgcg ccgcggcgcg cacccgggcg
     8821 gcgtcggtca cgaggaagtc gtcgccgatg atctgcacgg catggccgaa tctgcgggtg
     8881 aagcggagga agccctcggc gtcgtcctcg gcgagcgggt cctcgatcga gacgatcgga
     8941 tagcgggcga gccagccctc cagaagctcg atcatggcgt cccggccgag ctcgcgcccg
     9001 tcgcgcgcca gccggtagct cccatccctg ccgaactcgg aggccgccac gtcgaggctg
     9061 atcgccacct cgtcgccggg ccgccggccg gcgcgctcga tcgcctggac cagcgtctcc
     9121 agcgcctcct cgttgctggc gaagaccggc caccagccac cctcgtcggc gaccccggcg
     9181 aggcggccgc tctcggccat cagctcgccc gccgcacggt agacctcggc cgtccagtcg
     9241 agcgcctggg ccaccgtttc ggcccccggg cagacgatca tgaagtcctg gatgtcgacc
     9301 ctgcgggcgg catgggcgcc gccgccgaag atctggatct cgggcaaggg gagggtcacc
     9361 ggcccctggc ggtcgcccag caaatagcgc cagagcggct gaccggcggc ctccgccgcg
     9421 gcccaggcca cggccatcga gaccgcgatc atggcgttcg ccccgagccc gctgcggttg
     9481 gccgtgccgt cgagcgcgat cagcgcctcg tcgacggcga cctgatcgcg gccattgaga
     9541 ccgcgcagcg ccggggcgat ccgctcgccg acggccgcga cggcgccgcg cacgtcgtgg
     9601 ccgcccatga cgctgccgcc atcgcgccgg tcgaccgcct cggccgagcc cttggatgcc
     9661 ccggccgggg cgatcgcccg gccgacggcg ccgccctgga ggcgcacctc cgcctccacg
     9721 gtgggccggc cgcggctgtc gaagacgcgg cggccatgca catgctcgat cgaggtcgcc
     9781 cgggtcattg gccaaatccc gtgaaccagg cctcgcggaa tgcctcgagg cgcgccacgg
     9841 gctcggtcag atgatgaatg gcgaaggcac gcacgtccgc cttctccctg ccgctcgtca
     9901 gatactcgac cggcgacttg ccgtcgaccc gggccagcat caggccgggc aggagcgagg
     9961 tcgcccgttc ctcgaccgcc gccaccggct cccagtcgac atggccgaga taggcctcga
    10021 cgagccggcg gaacgcctcg gcgaagcgcc gtgcgtcggt cgggcgatgc cgggccttga
    10081 gcgccagatg gttgaggacg aaggcgagat cgaaggcggg gtcgccgtac cacgcgcact
    10141 cggcgtcgag cagcaccggc ccgagcctgc cgaccaggat gttcttcggg ctgacatcgc
    10201 catgcaccag gcagatcctc gtcgccgccg tccgccgggc gagggcgcgc agctgcgagg
    10261 cgaccatggg ctgggtctcg gcggtggcga ggagataggg ctcgagccgg atggcgtgga
    10321 agacctggtc gctgcggaac cgcgcggcga tgtcggcgtc attggccgtc aggctgtgga
    10381 tgcggccgag ccggtcgccg acggcggcgg cgacggccgg atcgatctgc cccgccatga
    10441 gcgccgtctt ccagagctcg taggccttcg ggtcgagata gcgcgtgacg atcgcggaag
    10501 ccgccgcgtc gacgtggatc agccccggca ccgccccggg caccaggcgg tcgaccacct
    10561 cgagataggc cgcctcgtag ctgttgcgct cgatcggtgc ccgccagtcg gcctgcacct
    10621 tgagcttggc gagcgcccgc ttcacgcagt attgcttgcc ggcgatgtcg acccgccaga
    10681 tatccgagga cacgccgccc gtgagcggca ccatcggtgg cggtgccccg agctcgagca
    10741 ggcccgcgcg gcgcagcacg gccatgatgt cgctgttcca accgacccgc atggcggcat
    10801 ccccgatcga cgctgggctg ccggccacgt caccgggtcg ggacgaccgg cagggaggat
    10861 gaccgtgcag cgtgtgctct ttcattacca agccggcgag cggctgacga gacggctgcg
    10921 ggcacttgcc gccgatgggc tggacgtgcg catcgtcgcc ccggaggacg cggacggtct
    10981 cgcccgcgag ctgtccggga cggagatcct ctggcatgcg ctggcgccgg tcacggcgga
    11041 gctgatcgag gcggcgcccc gtctccggct gatccagaaa tggggggtcg ggctcaacac
    11101 catcgacctc gcggcggcgc aggcccgggg catccaggtc gccaacatgc ccggcaccaa
    11161 cacccgggcc gtggtcgagc tgacgctgct cctgatcctc gcctgcctgc gtcgcctggc
    11221 cgaggtcgac ccggcgctcc gcggcggcca atgggcgccg ccgctggcca ccatggaagc
    11281 cctgggcgag ctcggcggcc ggcgcgtggg gctcgtcggc atgggcgccg tgccgcagat
    11341 gctggccccg atcctgaaag ccatgacggc cgagccggtc tactggagcc gggccgagaa
    11401 gccggatgcg ccggcccgct tcctgccgct cgccgagctc ctggcgacca gcgacgtcgt
    11461 ctcgctccat ctgcccctga ccgaggaaac gaccggcctc gtcgacgtgc gcgccatcaa
    11521 gcccggcgcc atcctcgtca acacggcgcg tggcggcctc gtcgacgagc cggcgctcgt
    11581 cggggcgctg cgctccggcc ggctcggcgc tgccgggctc gacgtcttcg aggccgagcc
    11641 gatcgacccg aataacccgc tcctggccct gcccaacgtc accgtgacgc cgcacctcgc
    11701 ctggctcacc ggcgagacac tcgagcgctc gctcgaggtc gcccgccaga acgcgctcaa
    11761 cctgcggacc ggtgagcctc tgctgttcag ggtggcctga acggcacggc ggcgcggcgt
    11821 cgccgtccga aaagtcccgg gcgcggcgcg cgattggctc tcccggttgg gtccgatcac
    11881 gcctatcaag gaagccgact ccagcggaaa tgcgctcatg accagcacga tccatgccac
    11941 cgtctgcccg cacgactgcc cgagcgtctg cgcgctcgag gtcgagaaga cggccgaggg
    12001 ccggctcggc aaggtcaagg gctcgaagcg caacagctac accgccggcg tcgtctgcgc
    12061 caaggtcagc cgctacgccg agcgctacca ccacaaggac cgcctggcct tcccgatgcg
    12121 gcgcaccggc gagcgcggct cgaccgactt cgcgcgcatc ggctgggacg aggccctgga
    12181 cgagatcgcc ctggcgttca cgcgggccga gcagcgccat ggctccgaga cggtctggcc
    12241 ctactggtat gccggcacga tgggcttcgt gcagcgcgac ggcgtccagc ggctgaccca
    12301 tgccaggcgc tggtcgcgct tcaagtcgac catctgcacc acgctcgccg acagcggctg
    12361 gcgggccgga tacggcaagc gctggggggt cccggccgag gaggcgggcg accatgccga
    12421 cctgatcggt gtctggggct gcaacccggt cgccacccac gtcaacctga tgagccacgt
    12481 ggcgcgggcg cgcaagcgtg gcgccctctt cttcgtggtc gatccctacc gcagcggcac
    12541 cgccgagcag gccgacctgc atctgccggt gcggccgggc acggatgggg cgctcgcctg
    12601 cgccgtcatg cacatcctct ttcgcgacgg ccatgcggac agggaatacc tggcgcgcat
    12661 gaccgatttc ccggccgagc tcgaagcaca cctggcgttg cgcgggccgg actgggcttg
    12721 cgccatcacc ggcctggacg tcgcgctgat cgagcgcttc gccgccctct acggcaacgc
    12781 tgcccgcccc tggatccgct tcggcttcgg cttcacccgc tcgcgcaacg gctcggccgc
    12841 catgcacgcc gcctcctgcc tgccggtggt caagggcgcc tggcagcacg agggcggcgg
    12901 cgggttatac aacatgggcg acctctacca ctgggacaag acgaccatcg agggccacga
    12961 cgtcatggat cccgcgaccc ggctgctcga ccagtcgcgg atcggcccgg tcctgaccca
    13021 cgacccgctc gacctgcagg gcgggccacc ggtcaccgcc ctcctggtcc agaacaccaa
    13081 cccgatgtgc atcgcccccg acctcggcaa ggtgcaccaa ggcttcgccc ggcccgacct
    13141 cttcgtcgcc gtgcaggagc agttcatgac cgagacggcg aagcaggcgg acatcctgct
    13201 gccggccacc atgttcctcg agcacgacga catctaccac gcctcgggcc actcgcgctt
    13261 ccagatcggc cgcaagatct tcgagcccca tgccgacgtg cgcagcaacc acttcgtcgt
    13321 ctgcgagctc gcgaagcgcc tcggcgccga gcatcgcggc ttccacatga ccgagtggga
    13381 gctgatcgag gacctgctcg cccgctcggg ctggccggat gcggcgacgg tgcacgagag
    13441 tggcggctgg gaggcgctgc ccgacgccga taccgcgcac tatcgcaacg gcttcccaac
    13501 ggccgacggc cggttccact tcagggccga ctgggcgctc cagggcgccg accatgcgcg
    13561 gatgccggct ctgcccgacc atctggcgat caccgacgag atcacggagg agcacccgtt
    13621 ccgcctcgtc gccgcccccg cccggcagtt cctcaacacg agcttcaccg agctggactc
    13681 cggccgcaag cgcgagggcc ggccgaccgc gatgatccac cccgattccc tcgaccggct
    13741 gggcatcgcc gaaggcgacc tggtggcgct cggcaatgcg cgcggcagtg ttcgcctgca
    13801 tgcggtggca cgggatggcc agcaccccgg cacgatcgtc gtcgagagca tctggccgaa
    13861 cccccactgg gtggacggga tcggcatcaa cctcctgctc ggcgccgacg cggcgccgcc
    13921 cgccggcggt gccgccatcc acgacacggc cgtctggctg cgacccgccg ccggctannn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc tgcgctacag ggcccggcgc
    14101 tgctccagcg cggtctcggg cggcaggtcg aagccgatca ggatggaata gtcggccgag
    14161 gtgcccgccg ccacgttctg cacccgctgc tcgagctgct cgcgcaggcg gacgcttccg
    14221 tccccggcac ccgcgggcac ggcggtcgcg aagctggcct tctcgatcac gttgccggcc
    14281 gggtcggcga gcaccacgaa ataggtgatc ggcgtgcctt cggggccatc caccacgatg
    14341 tcgaagctga tgtcgagatc gagggtctcg ccctgcagct ggcagaggcc atcgatgttc
    14401 tcgatattcg cgacgatctt ctgcccgttg gcgtcggtgc cgttgaaccg gtcgagcccg
    14461 tagatgatgg cgctgtccgg gcaaaccgcg gcctcctccg tcttgccggc gcagcccacc
    14521 ccaaaaagca ggatggggag caacagggcg gtcggcaggg ggagggcacc gcgcatggca
    14581 gccatcctca aagggccttg tgggtgccgt cgcagagcgg ctgcttgccg gaacgcttgc
    14641 acccgcagaa ccagagggca ccgtccctcg cggcggtgtg cttcaccggg gtgaagtccg
    14701 agcccttgtg gctgccgtcg cagaacggct gcgaccggct ctggccgcag gcgcaccacc
    14761 aataggcctt gccggccgtg acctcgacct tgaacggggc cttttgcggc atcaggggaa
    14821 gggtcgcggt ttcgctcatc ggtgctccgg cagaggctgg caggcaactg tggcgtagca
    14881 agcccattgg ccggcggcaa ggaccgtgcc gcccgcccga ggcacggtcg ggggcgaaag
    14941 cgccttgcgc gctggcggta cccccctact atgtgtcgat caggtgaaca gatgtacaga
    15001 gacgcagacc agaccacgcc ccccaacgga ggcgccaggg gtgtcggccg cccttggcga
    15061 agcccgcgcg ccgggccgcc gacccggatc gccgccctcg atctcggcac caacaactgt
    15121 cggctgctgg tcgccgagcc ccacgattcc accttcgatg tcgtcgacag cttctcgcgc
    15181 atcacccgcc tgggggaagg cctcgccttg agtggtggcc tgggcgagcc ggccatccgg
    15241 cgaaccctcg cggcgctggc ggtctgcaag cggatcatcg accgcaaccg ggtggcggaa
    15301 ttgcgctgcg tggccaccga ggcctgccgc cgcgccaaca acggtgccgc cttcatcgcc
    15361 caggtcgagc gcgagatcgg cctcaggctc gaggtgctga gccaggacga ggaggcgagg
    15421 ctcgctttgc tgggctgcct gcccctgatc gacgaggcgg ccgacgagct cttgatggtc
    15481 gatatcggcg gcggcagcac ggaggtgatc tggatggacc gggccggcgg cggacccggc
    15541 gaggcggtgt cgctgccgct cggcgtggtg accttggccg agtccttcgg ccgggagccc
    15601 gacgagagcg agtacgcggc catggtgcgg accgcgcgcc atgccctcgc cgaggtggcg
    15661 ctcggccatt tcgtgccgcg ctcgctcgac ccgcccgcgg tgcagatgct cggcacctcg
    15721 ggcacggtga cgacgctcgc tgccgtccat ctggggctgc gccgctacga ccgccgccgg
    15781 gtcgacggcc tgaccctgcc ctatgccgcg atcgccgccg tgaccgagcg acttcggcgc
    15841 ctggaccatc agggccgcgc cgcccatccc tgcatcggcc agggccgcgc cgatctcgtc
    15901 atcgccggct gcgccatcct ggatgcgatc cacgactgct ggccgatcag ccggctgcgt
    15961 gtcgccgatc gcggggtgcg cgaaggcatc ctcaacagca tcctcggcct cgatctgggt
    16021 gctgccctgc gccccgtggc ctgatcaggg tgggctgagc cgtgcagcgt acggaaaaga
    16081 cgaccctcaa gcgctcgcgc acccgccgcg ccagctcgac ccgctggctg cagcgccagc
    16141 tcaacgaccc gttcgtccag caggcgcagc gcgccggctg gcgggcgcgc tcggtcttca
    16201 agctcgagga gatcgacacc cgcttcaagc tgatcgccaa gggcgaggcg gtcgtcgacc
    16261 tcggtgccgc gccgggaagc tggagccagt acgcggcggc caagggtgcc cgcgtcctcg
    16321 ccctcgacct cctgccggtg gacccgatca atggcgtcga gatcatccag ggcgacttcc
    16381 tcgagccggg gatccaggcc aggctcctcg agcggctagg ctccaaggtg aagaccgtgc
    16441 tctcggacat cgccccggac gccaccggcc ggcgcctcgt cgaccggctg cgcgccgagg
    16501 ccgtgggcga ggcggtgatc gacttcgcca ccctcgtgct cgcccccgag ggtcaggtcc
    16561 tgatcaagct cgtgaagggc gccgagaccc gcgtcctcga ccgggcacga ccgcatttcg
    16621 ccaagctcag gctgatccgc ccgaaggcga cccgcagcga ttcctccgaa acctatctcc
    16681 tcgccagcgg ctttcgcccc accgagcccg aggtggatca gcccggcacg tagcgccgcc
    16741 agtcgtgctc ctcctcgaag cccagcacgt cgcggatctt gcggttcgaa atcggcgcct
    16801 cgaagtcgcc gagcggcctc gtgatcggcg tgcccggcgc atgctcgcgc aggaaagctt
    16861 ccgtcggctc gcgtgcggtg atggtgtcgt tgaccgcgtt gaagacctga aagccgagcc
    16921 cgtccctggc gaggcagcga tcgacgatct ggccgagatc gcgggcgtcg atatagctcc
    16981 aggcattgcg cttgcgcgac ggcggatcct ggaggaacgc ggcgaacctg tcgtactcgt
    17041 gcggctcgat gacgttgccg atccggagcg cgtagatgtc ggcacccgtc cgcatggcga
    17101 atgcccgacc cgtcttctcg ttcacgacct tggagaggcc gtagctgtcc atcggatcga
    17161 cgtcgtagtc ctcctcgagc gggaaggcgt ggaaatcctt gtcgccctcg gcgaagcaga
    17221 cgccgtaggt ggtctcgctc gacgcgatga cgatcttcct gatgccgagc ttcaccgccg
    17281 cctcgatgac gttgtaggtc gagatgacgt tgatccggaa cgtctcgttg tccggcctga
    17341 tcaggacgcg cggcaccgcg gcgaagtgga cgaccgcatc gaccggcgcc ggccccttgc
    17401 ccgtcgcgaa cccggcgaag ccgaaatgca tcgacaacgc gtcgaaggcc tggccgctgt
    17461 ccgtgaggtc ggcgatcacg gtgctcacgc ccggacggtc gagcggcacg aggtcgaggt
    17521 tcaggaccgc gtggccccgc tccagcagat agggcacgac gtgccgcccg gccttgccgc
    17581 tgccaccggt gaagacgatg cgcttgccca tgcctgttcc cgttgtccga ggcgccgtcc
    17641 tgccgatcag gccgccaccc gcggtctgcc tcggggaggc tcccctggca agccctccag
    17701 cacgtcctcg agcgagccga gccgctcgaa tgcggaaccg cgctcggcca gggggcggat
    17761 caggatctgc tcctcccggt cgattgccac gagcagcgga acggccgtct cgtccgccat
    17821 gatggcgagc gcctcggcga atcgcccctc gcagagctcg gcgtcacccg gccggccatc
    17881 cccgtcgatt ggcaggacga gcaggcgacc atcggcaagg ctcgccgcca gcaccacccg
    17941 gcgctcggca tcgaagccga cgaccgggcg gccgaccggc tctcggtgca ggcgcaccgg
    18001 cgcgtccggc acccgggcgg agcggtcgag gcggaccagc c
//
LOCUS       DAOTFI010000091        17985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3755_length_17985_cov_7.003291, whole genome shotgun sequence.
ACCESSION   DAOTFI010000091 DAOTFI010000000
VERSION     DAOTFI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17985
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_17985_cov_7.003291"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="P5558_10940"
     CDS             <1..134
                     /locus_tag="P5558_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HRY24869.1"
                     /translation="ELDRKVGEPIDIYVNNRLVARGEVLIVDDHLGITMTEIIKSDS"
     gene            134..964
                     /locus_tag="P5558_10945"
     CDS             134..964
                     /locus_tag="P5558_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY24870.1"
                     /translation="MQRRASWQTRRRAPAGSFSRLDPAGAVGLVAAFAVIGLAIGTGS
                     APARFVDLPSVLIVLGGTFAVTFASFSLHEFARLPRVVGAALSLTPPCALPVVANDAL
                     TLAEAARQETLPGLERRVEALARHGFLHRGLMLLLEGADRDAIERTLRREIAAMVERQ
                     RSTVGILRRAAEVAPAMGLIGTLIGLVQMLGHLDDPDEIGPAMAIALLTTLYGALLAH
                     ALFTPLAEKLQRRFEAELAGAELQLVAALAIADRENPRRLEAALNGLLAPDERLRRFD
                     "
     gene            994..2337
                     /locus_tag="P5558_10950"
     CDS             994..2337
                     /locus_tag="P5558_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY24871.1"
                     /translation="MGTRLLVIGPDGHEVAAAVGMAVASGAKIRRAADRAAALALLRA
                     GQGADLVLVDLDADVAGLIRDLRDERFHLPVVAYGIAADPVAAVAAIRAGAREFLPLP
                     PDAELIAALLAAAATPMRELVCADPAMRRVLDLAGQFAAADASVLITGESGTGKEMVA
                     RLLHAGSRRSGGPFISVNCAAIPEQLLESELFGHEKGAFTGAVARRVGKFEEASGGTL
                     LLDEVTEMDPRLQAKLLRAIQEREIDRLGGGKPVRIDIRLIATSNRDMEEAVAAGAFR
                     DDLYFRLNVLTLHLPPLRERPGDIAALAAHFAAKHARTNGRPVPEIEPAALARLQAHH
                     WRGNARELENCMHRAVLLCQSGRIGPAEIILGGRAGPVAEARAGGAAPVGRSLADVER
                     DLIIDTLGHTFGNRTHAATMLGISIRTLRNKLRQYSEAGLVVPASTGPAPAGDAA"
     gene            2360..4450
                     /gene="flhA"
                     /locus_tag="P5558_10955"
     CDS             2360..4450
                     /gene="flhA"
                     /locus_tag="P5558_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRY24872.1"
                     /translation="MAIQLRATTDQMVPMLWRALRQSDVLMALGVIAILVVLILPLPP
                     VLLDLLLAVSIMFSVLVLLTSLFIEKPLDFSAFPTVLLIATMLRLSLNLASTRLILAN
                     GHEGPDAAGGVIEAFGGFIMSGNYVIGIIVFAILVIVNFVVITKGSGRIAEVAARFSL
                     DAMPGKQMAIDADLSAGLIDEAAARSRRKELEEESAFFGAMDGAAKFVRGDAIAGLLI
                     TFINLLAGLVIGVGQMGISFGEAAESYTILTVGDGLVSQIPALIVSTAAGLLVSKAGV
                     TGRADKALLKQMGAFPKSLGVTSGLMFALAVLPGLPVLPFVIIGGGAGWLAWRSSRAA
                     VAAETAENLPEPAPARPPEEPIATALQIDPIRLELGYGLLPLINESGAGRLTEQIKAL
                     RRQLAGELGFVMPSVRIQDNIQLPANTYVLKVKEMEAARGDIRPNMLLVMHPQGAAIE
                     LPGEETVEPTFGLAAMWVGQGLREEATFKGLTVVDPATVVTTHLTEIVKDNLAEMLSF
                     AETQKLLAELDAQYQKLLADLVPQRLSTAGIQRVLQGLLAERVSIRDLPLILEAISEA
                     VQFTQHAVFIIEHVRARLARQITGSITGPGGYVPLLTLSPVWEQAFGESLVGQGEDRQ
                     LAMSPSQLQDFIRALRDAFEAQAMRGELPVLVTSPAIRPYVRSIVERFRPATVVLSQN
                     EIHPKAKLRTLGQV"
     gene            4454..5401
                     /locus_tag="P5558_10960"
     CDS             4454..5401
                     /locus_tag="P5558_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24873.1"
                     /translation="MRLRVFEGRTVQEAMAQLRRAMGDDAVILATEEGAGVVRLTAAR
                     DKGPDDLADILTAGTSLEVIERLAVVLRGHRVAAALIERICVAARESGSRDAEEVLAF
                     ALAAHCRFAAIGHPPGRLAGRPLVVAGAPGVGKTVMVARLAAAARLAGRPVRVASTDR
                     ARRGGAAQLEALLGAMGVELEGLPAAPAAVDPACLLLVDTEAAHPLRPDALAGAAGVA
                     QQLGAELLPVFAAETCALEAHDQALAWRALGATRHLVARLDTVRRLGLLLSVADAGLA
                     CAGAGISPFVGEPLKPLTAANLARLLLHRAASFVQEGSA"
     gene            5398..6147
                     /locus_tag="P5558_10965"
     CDS             5398..6147
                     /locus_tag="P5558_10965"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HRY24874.1"
                     /translation="MTGLTVLASGKGGVGKTFLAVSLAHALTHLGRRVLLVDADLGLA
                     NIDVQLGFDEPIGLGRAIAGGLDPGALVQDRGGGLSTLTGAGTGQSLDGLATARLEEA
                     VERLAALATSFDEVLVDLPSGLDRVGPAFIGKARRLLLVGSNEPTSLTDNYALLKLTR
                     GAAPRPLFIANAVVDAAEGRVAHATLARACRHFLDLDCVYLGAVRHDRRVPEAIRRQM
                     PLCLRSPQAPAAADIAVLASRLSAEPTAPAR"
     gene            6144..7088
                     /locus_tag="P5558_10970"
     CDS             6144..7088
                     /locus_tag="P5558_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24875.1"
                     /translation="MSTVGPAAGPPVLPAAGTGAGGVGRPTAGHQTPTGAGAPVPTSP
                     ATLRAPGPWLPATLLPAGAASSGLAVELRLATSPATAGALLGRVAAGGSALALDQGGH
                     LLAMTLRLGDLPEGARLPIELRSPATGATVADRAGLERLVRSTAEPPPPAVSGAGLAA
                     RLLGEWRRTRTDGRLDPPAEATARPAAAADGTGGWRSLFDLAGAGSLPVAPVLLWQHR
                     PEREGRPEEPGVEHLRLVVELSRLGRVSIDLRVDAHALATSVTSAEPLDAELRAGIGD
                     VYAAAVELAGRSGSLAFRVAPSAAAVAPHAGPMDDLTV"
     gene            7195..8349
                     /locus_tag="P5558_10975"
     CDS             7195..8349
                     /locus_tag="P5558_10975"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRY24876.1"
                     /translation="MQRPEARPLNPCFGSGPTAKRPGWSIDVLRPALVARSHRSAAAK
                     ARLAAVIDRSKAILGMPADYRLGIVPASDTGAFEMAMWSLLGARGVDVVAFESFGAGW
                     LADATGELRLEDLRRFEADYGALPDLAGTSPERDIVFTWNGTTSGVVVPDGDWIAADR
                     SGLTLCDATSAVFAMQLPWEKLDVVTWSWQKVLGGEAQHGMLALSPRAVERLQGWTPP
                     WPMPKLFRLAKGGKLAEGVFRGETINTPSMLAVEDVLDALAWAEGQGGLDGLIARSRA
                     NLAAVAKWVEGSAWAGFLATDAAVRSSTSICLKITDPGIARRTPAEQNAFVKAMVKRL
                     EDEGVAFDIAGYRDAPPGLRLWGGATVETCDIERLLPWLDWAFAEAKAAA"
     gene            8393..9970
                     /gene="serA"
                     /locus_tag="P5558_10980"
     CDS             8393..9970
                     /gene="serA"
                     /locus_tag="P5558_10980"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRY24877.1"
                     /translation="MPRVLIADKLSPAAVAIFAERGIEADVKTGLDPAELAGIIGGYD
                     GLAIRSSTRVTADLLAAATRLKVVGRAGIGVDNVDVDAATEHGVVVMNTPFGNSVTTA
                     EHAIAMLLATARQLPAADRSTRAGKWEKSRFVGVELASKTLGIIGCGNIGSIVANRAQ
                     GLKMRVIAFDPFLSAERARDLGIEKVDLDELLERADFISLHTPLTEQTRNILSAESLA
                     RTRKGVRIVNCARGGLVDEAALAALIESGHVAGAALDVFATEPARESPLFALDDVVVT
                     PHLGASTAEAQEKVALEIAEQMSDYLLKGAIVNALNMPSVTAEEAPRLKPYMLLAEQL
                     GSFAGQLTETGLEGVTIRYAGEVARLNTRPLTQMILKGLLSPLLASVNMVNAPAVARS
                     RDIGVTTVETEDLEGYQTLIAVEVETERGRREVCGTLIHDGRPRVVRIRGIEIEARLA
                     DHMLYLRNSDTPGFIGALGGLLGDAGVNIATFHLGRDRPGGDAIAMLEVDGPVPEAVL
                     RRIRALPQVLQVKALAF"
     gene            complement(9951..11810)
                     /gene="recQ"
                     /locus_tag="P5558_10985"
     CDS             complement(9951..11810)
                     /gene="recQ"
                     /locus_tag="P5558_10985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRY24878.1"
                     /translation="MPLANAGIRLPDHPDPETALHEIFGFRQFRPGQRAIVESLLRGE
                     NLLAVMPTGGGKSLCYQLPAVLKDGLSLVVSPLIALMDNQVAQLRLLGIEAAAVHSGT
                     DRGERGRIWQSIKDGVLKLLYLSPELLGSERLQAALDRLKLSQIIVDEVHCLSQWGHD
                     FRPDYRNLGQLPARYPDACIAGFTATADVATRADIESQLFQHRPGRVFVQGFDRPNIS
                     LKVLAKERAERQLVELIEPFRGRQGIVYRLARRDTEATAAALRDAGFPAVAYHAGLDS
                     GLRRQLLDRFLSEPDLVVVATIAFGMGIDKADIRFVVHVDLPSNLEAYYQEIGRAGRD
                     GLPAEAILLHGFDDIRRRRWLIDSGNADAAVKRVEHRRLDALLSFTETSGCRKQTLLG
                     YFGEAAGPCGACDRCLNPPALVEAGDLARALLGAIDATGRRFGQAHLIDILRGEATEK
                     VATLGHDRLAAFGAAADMDKRVLRGAVRQLYASGVVEVDIAGHGALRPGRKAADVLRG
                     EAAVPLDLTAPPTKARRGRKTAAGTSAAPVEPAAMELLAALKVLRLEIAREIDKPAYI
                     VFSDATLVDMAQKRPTSLEAFLAVDGVGQKKCELYGSRFIEAIEAEPIKRPAP"
     gene            11913..>12469
                     /locus_tag="P5558_10990"
     CDS             11913..>12469
                     /locus_tag="P5558_10990"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRY24879.1"
                     /translation="MTNVAVVGSQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLV
                     VGGVTYKLSLLPSGVVRKGKLSIIGNGVVVDPWALLEEIERLRQQGVAIGSETLRVAE
                     NATLILPFHGELDRAREEARDKANSGGGKIGTTGRGIGPAYEDKVARRAIRVCDLADE
                     AVLEQKLAELLLHHNALRRGFGVDE"
     assembly_gap    12470..12569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12570..12998
                     /locus_tag="P5558_10995"
     CDS             <12570..12998
                     /locus_tag="P5558_10995"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRY24880.1"
                     /translation="TLGERGREFGTVTGRKRRCGWFDAVLVRQAVKTGGIRGLALTKL
                     DVLDGFERLKICTAYNLNGQTLHHLPAGMAAQAAVRPIYEEIEGWSESTAGARSFVDL
                     PAQAIKYVKRLEELVEAPVALLSTSPARDDTILVTDPFAD"
     gene            13144..13620
                     /locus_tag="P5558_11000"
     CDS             13144..13620
                     /locus_tag="P5558_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24881.1"
                     /translation="MISRAGQDPQVAFRAGLAHAATPGNAGPGERLVLRMQRLPGLVE
                     PGAAFEVGRALRAIARLFDRAAAPVVMVEQFRDNLVGAAIAQRGRLERNGRCRRGWNL
                     QDTIEMFVEMYERLLGQSATGYAEIRGYVFRAAAGPAPLMPLPASRVPPRFFRRAA"
     gene            complement(13636..15816)
                     /locus_tag="P5558_11005"
     CDS             complement(13636..15816)
                     /locus_tag="P5558_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRY24882.1"
                     /translation="MSDPTPLRLGDPERMVDAIIERVGRRIVVGAPLGLGKPVHLLNA
                     LYHRAAADRTLELRIVTGLSLARPQAKSDLERRMLGPIVERLFGDCPELAYVGPYSRN
                     ALPPNIRVTEFYMRAGAFLNNATAQQHHISSNYTHVARDLLLQGVNVFVQAVAKRCRD
                     GQTTYSLSSNSDSLDLFRLLDASDPERRVVRVAQVNERLPFMGRDALVSASTFDLVLD
                     HRSLDHDLLSLPAGPVHTADYLIGLAASSLIRDGGTVQIGIGSLGDAIAHALCLRHRE
                     NATYQEALRATGLAGRCGDSIAAIGGTAPFETGLYGATEMFADCFRHLYRAGILRREV
                     YDDLAIQRLVNAGLISAEVTLGTLDALVEAGAVPSPLDAAAVDHLVRRGVLREGIAFA
                     DGELVTPSGRRLSPDLADPETRASIEAECLGDRLAGGSILHAGFFIGPRAMYDLLREL
                     DEGERAKFNMTGIGFVNQLYGHEALAAAQRRHARFVNSAMMMTLCGAACSDGLANEQI
                     VSGVGGQYNFVAMAHALEGARSILMLRSTRTKAGKVSSNIVWSYGHTTIPRHLRDIVI
                     TEYGIADLRGRQDHECIAALLNISDSRFQEDLRAEAVASGKLASTHRIPDRHRANTPA
                     RLEAELKPLRAAGHFPTFPFGSELTHEEQVLGHVLKGLKARMGRRLGLAGIVKETVGA
                     GLDVPDEALPYLARLGLDRPSSLRETMLQRILVAELRSGGFIRA"
     gene            complement(15822..16250)
                     /locus_tag="P5558_11010"
     CDS             complement(15822..16250)
                     /locus_tag="P5558_11010"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRY24883.1"
                     /translation="MAEDTKAVVRRLLEEVWGAGDLSLLPELVAADCVVDASSAGLRL
                     EGIDQYRQFVATYHALYGEAVFSIESQVAEGDLVASRWTITMELPEDADPAMPRAASG
                     MSFHRLKDGRVVESWDSWDVLAASHLQQEVDLMERLTLGF"
     gene            complement(16317..16745)
                     /locus_tag="P5558_11015"
     CDS             complement(16317..16745)
                     /locus_tag="P5558_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRY24884.1"
                     /translation="METTLPVTDKRPGVGCGAVILRGDAILLIERRRPPEAGHWGLPG
                     GKVDWLEPSLEATRREVAEELGIVLRDPTFLCMVDLIDEAAGHHWVAPVFLCREFTGE
                     PRLMEPLKHGGFGWFPLDRLPEKLTTPTRAALAALRRRSD"
     gene            16987..17517
                     /locus_tag="P5558_11020"
     CDS             16987..17517
                     /locus_tag="P5558_11020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRY24885.1"
                     /translation="MAQRYATALLDLAREEKKLDAVAADLRGLVALLGESRELRRLVE
                     SPVISREEQGRAILAVADKAGTEPLTAKFLGLLASRRRLFALPGIARAYAAMLAEERG
                     EVAAEVVSAVPLGEAELDSLKNAIAGHVGQSVNVETRVDPALLGGVVVRVGSRMLDAS
                     IRTKLQHLEQSMRGMG"
     gene            17517..>17985
                     /locus_tag="P5558_11025"
     CDS             17517..>17985
                     /locus_tag="P5558_11025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386490.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRY24886.1"
                     /translation="MDIRAAEISAILKEQIANFGTEAEISEVGRVLSVGDGIARCYGL
                     DQVQAGEMVEFEDGTRGMALNLEEDNVGIVIFGDDRNISEGAIVKRTQSIVDAPVGKG
                     LLGRVVDGLGNPIDGKGPIDATERRVVDVKAPGIMPRKSVHEPMQTGLKAIDAL"
BASE COUNT         2478 a         6477 c         6338 g         2592 t
ORIGIN      
        1 tcgagctcga ccgcaaggtg ggcgagccga tcgacatcta cgtcaacaac cgcctcgtgg
       61 cgcgtggcga ggtgctgatc gtcgacgacc acctcggcat caccatgacc gagatcatca
      121 agagcgacag ctgatgcagc gacgggcatc ctggcagacg cggcggcggg caccggccgg
      181 cagcttcagc cggctcgacc cggccggggc cgtcgggctg gtcgccgcct tcgcggtgat
      241 cggcctcgcc atcggcaccg gctcggcgcc ggcccgcttc gtcgacctgc cgtccgtgct
      301 gatcgtgctc ggcggcacct tcgccgtgac cttcgcgagc ttctcgcttc acgagttcgc
      361 gcgcctgccc agggtcgtcg gcgccgccct gtcgctcacc cccccatgcg ccctgccagt
      421 ggtcgccaac gacgcgctca ccctggccga ggcggcccgc caggagacgc tgcccgggct
      481 cgagcgccgg gtcgaggcgc tggcccgcca cggctttctc catcgcggcc tcatgctgct
      541 gctcgagggg gccgaccggg acgccatcga gcggacgctg cgccgcgaga tcgccgccat
      601 ggtcgagcgc cagcgcagca cggtcggcat cctgcgccgc gccgccgagg tggcgcccgc
      661 catggggctg atcggcacgc tgatcgggct cgtgcagatg ctcggccatc tcgacgaccc
      721 ggacgagatc ggcccggcca tggcgatagc gctcctcacc acgctctacg gcgcgctcct
      781 ggcgcatgcc ctcttcaccc cgctcgccga gaagctgcag cgccgcttcg aggccgagct
      841 cgccggggcc gagctgcagc tcgtcgccgc cctcgccatc gccgaccggg agaacccgcg
      901 gcggctggag gcggccctca acggacttct ggcccccgac gagcgcctca ggcgcttcga
      961 ctgaggagcc caagcttgaa caagggaagc gacatgggaa cgcgcctatt ggtcatcggc
     1021 ccggacgggc acgaggtcgc cgcggctgtc ggcatggcgg tggcgagcgg ggcgaagatc
     1081 cgccgtgcgg cagaccgcgc ggcggcgctg gccctgctgc gcgccggcca gggtgccgat
     1141 ctcgtcctcg tcgatctcga tgccgacgtc gccggcctca tccgcgacct gcgcgacgag
     1201 cgcttccatc tgccggtcgt ggcctacggg atcgccgccg acccggtggc ggcggtggcc
     1261 gcgatccgcg ccggggcccg ggagttcctg cccttgccgc ccgatgccga gctgatcgcg
     1321 gccttgctgg ccgccgcggc gaccccgatg cgcgagctgg tctgcgccga cccggcaatg
     1381 cgccgcgtgc tcgatctcgc cggccagttc gccgcggcgg atgcgagcgt gctgatcacc
     1441 ggcgagagcg gcaccggcaa ggagatggtg gcgcgcctgc tgcacgccgg cagccgccgg
     1501 agcggcggcc cgttcatctc cgtcaattgc gcggcaatac ccgagcagct gctcgagtcc
     1561 gagctcttcg gccacgagaa gggtgccttc accggtgccg tcgcccggcg ggtcggcaag
     1621 ttcgaggagg cgagcggcgg cacgctcctg ctcgacgagg tgaccgagat ggacccccgg
     1681 ctgcaggcca agctgctgcg agccatccag gaacgcgaga tcgaccggct cggcggcggc
     1741 aagccggtgc ggatcgacat ccggctgatc gccaccagca accgcgacat ggaggaggct
     1801 gtcgccgccg gcgccttccg cgacgatctc tatttccgcc tcaacgtgct gacgctgcat
     1861 ctgccgccgc tccgcgagcg gccgggcgac atcgcggctc tcgccgccca tttcgccgcc
     1921 aagcacgcgc gcaccaatgg ccgcccggtc cccgagatcg agcccgcggc cctggcccgg
     1981 ctgcaggcgc atcactggcg cggcaatgcc cgtgagctcg agaactgcat gcaccgggcg
     2041 gtgctgctct gccaaagcgg gcgcatcggc ccggccgaga tcatcctcgg cggccgggcg
     2101 gggcccgtgg ccgaagcgcg ggccggcggt gccgcaccgg tcggccgcag cctcgccgac
     2161 gtcgagcgcg acctgatcat cgacacgctg ggccacacct tcggcaaccg gacgcacgcg
     2221 gcgaccatgc tcggcatctc gatccgcacg ctgcgcaaca agctcaggca gtacagcgaa
     2281 gcggggctcg tcgttccggc atcgaccggc ccggcacccg ccggcgacgc ggcctgaggg
     2341 cggggggcgg cgagcgacga tggccattca actgagagcg accacggacc agatggtgcc
     2401 catgctctgg cgggccctgc gccagagcga cgtgctgatg gcgctcggcg tcatcgccat
     2461 cctggtcgtg ctcatcctgc ccttgccgcc ggtcctgctc gacctcctgc ttgccgtctc
     2521 gatcatgttc tcggtgctgg tcctcctgac ctcgctcttc atcgagaagc cgctcgactt
     2581 cagcgccttt ccgaccgtgc tcctgatcgc gaccatgctc cggctgtcgc tcaacctcgc
     2641 ctcgacccgg ctgatcctcg ccaatggcca cgagggaccg gatgccgcgg gcggcgtgat
     2701 cgaggccttc ggcggcttca tcatgtcggg caactacgtc atcggcatca tcgtcttcgc
     2761 catcctggtg atcgtcaatt tcgtcgtcat caccaagggc tcggggcgca tcgccgaggt
     2821 cgccgcccgc ttctcgctcg acgccatgcc gggcaagcag atggcgatcg acgccgacct
     2881 ctcggccggg ctgatcgacg aggccgccgc ccgctcgcgc cggaaggagc tggaggagga
     2941 gagcgccttc ttcggcgcca tggacggtgc cgccaagttc gtccgcggcg acgccatcgc
     3001 cgggctcctg atcaccttca tcaacctgct cgccggcctc gtcatcggcg tcggccagat
     3061 gggcatcagc ttcggcgagg cggccgagag ctacaccatc ctcaccgtcg gcgacggcct
     3121 cgtctcgcag atccccgccc tcatcgtctc gaccgccgcc ggcctcctgg tctccaaagc
     3181 cggcgtcacc ggccgggccg acaaggcgct gctcaagcag atgggcgcct tccccaagtc
     3241 gctcggcgtc accagcgggc tcatgttcgc gctcgccgtg ctgcccggcc tgccggtcct
     3301 gccgtttgtc atcatcggtg gcggcgccgg ctggctcgcc tggcggtcgt cgcgcgccgc
     3361 ggtcgccgcc gagacggcag agaacctgcc cgagcctgcc ccggcgcggc cgccggagga
     3421 gccgatcgcc accgccctgc agatcgaccc catccgcctc gagctcggct acggcctcct
     3481 gccgctgatc aacgagagcg gtgcggggcg gctcaccgag cagatcaagg ccctgcgccg
     3541 ccagctcgcc ggcgagctcg gcttcgtcat gccgtcggtg cgcatccagg acaacatcca
     3601 gctcccggcc aacacctacg tcctcaaggt caaggagatg gaggcggcgc gcggcgacat
     3661 ccgccccaac atgctgctcg tcatgcaccc gcagggtgcg gcgatcgagc tgccgggcga
     3721 ggagacggtc gagccgacct tcggcctcgc cgccatgtgg gtcggccagg gcctgcgcga
     3781 ggaggcgacc ttcaagggcc tgaccgtggt cgacccggcg acggtcgtca ccacccacct
     3841 gaccgagatc gtcaaggaca acctcgccga gatgctgagc ttcgccgaga cgcagaagct
     3901 cctggccgag ctcgacgcgc agtaccagaa gctgctcgcc gacctggtcc cgcagcgcct
     3961 ctcgacggcc ggcatccagc gcgtgctgca gggcctcctg gccgagcggg tgtcgatccg
     4021 cgacctgccc ttgatcctcg aggcgatcag cgaggccgtc cagttcaccc agcacgccgt
     4081 cttcatcatc gagcatgtcc gcgcccggct ggcccgccag atcaccggct cgatcaccgg
     4141 ccccggcggc tacgtgccgc tgctgaccct ctctccggtc tgggagcagg cgttcggcga
     4201 gagcctcgtc ggtcagggcg aggaccgcca gctcgccatg tcgccgagcc agctccagga
     4261 cttcatccgc gcgcttcggg atgccttcga ggcgcaggcg atgcgcggcg agctgccggt
     4321 cctcgtcacc tcgccggcga tccggcccta tgtccgctcg atcgtcgagc gcttccgccc
     4381 ggccaccgtc gtgctgtcgc agaacgagat ccaccccaag gccaagctgc gcacgctggg
     4441 gcaggtctga gccatgcgcc tgcgggtatt cgaggggcgg acggtgcagg aggccatggc
     4501 gcagctgcgt cgcgccatgg gcgatgacgc ggtgatcctg gcgaccgagg agggggccgg
     4561 ggtcgtgcgc ctcaccgcgg cccgggacaa gggtcctgac gatctcgccg acatcctgac
     4621 cgccggcacc tcgctcgagg tgatcgagcg gctcgcggtc gtgctgcgcg gccaccgggt
     4681 cgccgcggcg ctgatcgagc ggatctgcgt cgccgcccgc gagagcggct cgagggacgc
     4741 cgaggaggtg ctcgccttcg cgctcgccgc ccattgccgc ttcgccgcca tcggccaccc
     4801 gcccggccgg ctggccggcc ggccgctggt ggtggcggga gcacccggcg tcggcaagac
     4861 cgtcatggtg gcgcggctgg cggcggccgc ccggctcgcc ggccggccgg tccgcgtcgc
     4921 cagcaccgac cgtgcccgcc gtggcggcgc ggcccagctc gaggccctgc tcggcgccat
     4981 gggggtcgag ctcgagggcc tgcccgcggc cccggccgct gtcgacccgg cttgcctgct
     5041 gctggtcgat accgaggccg cccatccgct ccgccccgat gcgctggcgg gcgcagccgg
     5101 ggtggcgcag caactgggtg ccgagctgct gccggtcttc gcggccgaga cttgtgcctt
     5161 ggaagcgcac gatcaggcgc tcgcctggcg ggcgctgggg gcgacgcgcc atctcgtcgc
     5221 ccggctcgac acggtgcgcc ggctcggcct tctcctctcc gtcgccgatg ccgggctggc
     5281 ctgcgccggg gccggcatct cgcccttcgt cggcgagccc ctgaagcctc tcacggccgc
     5341 caacctcgcg cggctgctcc tgcatcgcgc cgcctcgttc gtccaggagg gctcggcatg
     5401 accggcctca ccgtgctggc ctccggcaag ggcggggtcg gcaagacctt cctcgcggtc
     5461 tcgctggccc atgcgctcac ccatctgggg cgtcgcgtgc tcttggtcga cgccgatctc
     5521 ggcctcgcca atatcgacgt gcagctcggc ttcgacgagc cgatcggcct cggccgggcg
     5581 atcgccggcg ggctcgatcc gggggcgctg gtccaggacc ggggaggcgg cctctcgacc
     5641 ctcaccggcg ccggcaccgg ccagagcctc gacggcctcg ccaccgcccg gctggaggag
     5701 gcggtcgagc ggcttgccgc cctggccacg agcttcgacg aggtgctggt cgacctgccg
     5761 agcggcctcg atcgcgtcgg ccccgccttc atcggcaagg ccaggcggct gctcctcgtc
     5821 ggcagcaacg agccgacctc gctcaccgac aactatgcgc tcctcaagct gacccggggt
     5881 gccgcacccc gcccgctctt catcgccaac gcggtggtcg atgcggcgga ggggcgggtg
     5941 gcgcatgcga cgctggcccg ggcctgccgc cattttctcg atctcgactg cgtctatctc
     6001 ggtgccgtgc gccacgaccg ccgggtgccc gaggcgatcc gtcggcagat gccgctctgc
     6061 cttcgctcgc cgcaggcgcc ggccgccgcc gacatcgccg tcctggcgag ccgtctcagc
     6121 gccgagccga ccgctccagc tcggtgagca cggtcggccc cgccgccggc cccccggtac
     6181 tgccggcagc cggcaccggc gccggtggtg tgggccggcc gaccgccggg caccagacgc
     6241 ccacgggtgc cggggcgcct gtgccgacca gccccgccac gctgcgcgcc cccggcccct
     6301 ggctgcccgc caccctgctg ccggccggtg ctgccagctc gggccttgcg gtcgagctgc
     6361 ggctcgccac gtcgcccgcg acggccggag cactcctcgg acgggtcgcc gccggcggca
     6421 gcgcgctggc gctggaccag ggcggccatc ttctggcgat gaccctgcgg ctcggtgacc
     6481 tgcccgaggg tgcccgactg ccgatcgagc tgcgatcacc ggccaccggt gcaaccgttg
     6541 ccgatcgggc cggcctcgag cggctggtgc gctcgaccgc cgaaccgccc ccgccggcgg
     6601 tgtcgggagc agggctcgcg gcgcggctcc tgggcgaatg gcgccgaacc cgaactgacg
     6661 ggcggctcga tccgccagcc gaggcgaccg cccggccagc tgccgcggcc gatggcacgg
     6721 gcggctggcg gagcctcttc gatctcgccg gcgccggatc gctgccggtc gcacccgtgc
     6781 tgctctggca gcaccggccg gaacgcgagg gccgccccga ggagcccggg gttgagcatc
     6841 ttcgcctcgt cgtcgagctc agccggctcg gtcgggtctc gatcgacctg cgcgtcgatg
     6901 cccatgcact cgccacgtcg gtcaccagcg cagagccgct cgacgccgag ctgcgcgccg
     6961 gcatcggcga cgtctacgcc gcggcggtgg agctcgccgg gcgctcgggc agcctcgcct
     7021 ttcgcgtcgc accatccgct gcggccgtgg cgccgcacgc cggcccgatg gacgacctca
     7081 ccgtctgagg tcgaggccgc gaccacgggt ttgttgcagc gcagcgtcat ccccactagc
     7141 ttctgcgccg cttcatcccc gcgcaccctt ggcccgagca tccctggagc gttcatgcag
     7201 cgtcccgagg cccgccctct caatccctgc ttcggctcgg gcccgaccgc caagcggccg
     7261 ggctggtcga tcgacgtgct gcggccggcc ctggtcgccc gctcgcaccg ctcggccgcc
     7321 gccaaggccc ggctggctgc ggtcatcgac cggagcaagg cgatcctcgg catgccggcg
     7381 gactaccgcc tcggcatcgt ccccgcctcc gacaccggcg ccttcgagat ggcgatgtgg
     7441 tcgctgctgg gggcgcgggg cgtcgatgtc gtggccttcg agagcttcgg cgccggctgg
     7501 ctcgccgatg cgacggggga gctccggctc gaggatctgc gccgcttcga ggccgactac
     7561 ggtgccctgc cggacctcgc cggcacctcg cccgagcgcg acatcgtctt cacctggaac
     7621 ggcacgacct cgggcgtggt cgtgccggac ggggactgga tcgccgccga ccggagcggg
     7681 ctcacgctct gcgacgccac ctcggcggtc ttcgccatgc agctcccctg ggagaagctc
     7741 gatgtcgtca cctggtcctg gcagaaggtg ctgggcggcg aggcgcagca cggcatgctg
     7801 gcgctgagcc cgcgcgccgt cgagcggctg cagggctgga ccccgccatg gccgatgccc
     7861 aagctcttcc gcctcgccaa gggcggcaag ctggccgagg gcgtcttccg gggtgagacc
     7921 atcaacacgc cctccatgct ggccgtcgag gacgtcctcg acgcgctcgc ctgggccgaa
     7981 gggcagggcg ggctcgatgg cctgatcgcc cgctccaggg ccaatctcgc cgccgtcgcc
     8041 aaatgggtcg aaggctccgc ctgggccggc ttcctcgcca cagacgctgc cgtccgctcc
     8101 tccacctcga tctgcctgaa gatcaccgat cccggaatcg cccggcggac gccggccgag
     8161 cagaacgcct tcgtcaaggc gatggtgaag cggctcgagg acgagggtgt ggccttcgac
     8221 atcgccggct accgcgacgc cccgccgggc cttcgcctct ggggcggggc cacggtcgag
     8281 acatgcgata tcgagcggct gctgccctgg ctcgactggg ccttcgccga agccaaagcg
     8341 gcggcctgaa gtcgccgctc cccatgccac ttcccactga cggattgtcc ccatgccccg
     8401 cgtgctgatc gccgacaagc tcagccccgc cgccgtcgcc atcttcgccg agcgcggcat
     8461 cgaggccgat gtgaagaccg gcctcgatcc ggcggagctc gccggcatca tcggcggcta
     8521 cgacggcctc gccatccgct cctcgaccag ggtgacagcc gatctcctgg ccgccgccac
     8581 gcgcctcaag gtggtcggcc gggccggcat cggggtcgac aatgtcgatg tcgatgccgc
     8641 caccgagcat ggcgtcgtcg tcatgaacac gcccttcggc aactcggtca ccacggccga
     8701 gcacgccatc gccatgctgc tcgccaccgc ccggcagctc cccgctgccg accgctcgac
     8761 ccgcgccggc aagtgggaga agtcgcgctt cgtcggcgtc gagctggcgt cgaagacgct
     8821 cggcatcatc ggctgcggca atatcggctc gatcgtcgcc aaccgggcgc aggggctgaa
     8881 gatgcgggtt atcgccttcg accccttcct ctcggccgag cgggcccgcg acctgggcat
     8941 cgagaaggtc gacctcgacg agctgctcga gcgcgccgac ttcatctcgc tgcacacgcc
     9001 cttgacggag cagacgagaa acatcctgag cgcggagagc ctcgcccgca cgagaaaagg
     9061 cgtgcgcatc gtcaactgcg cgcgcggcgg cctcgtcgac gaggcggcgc tcgcggcgct
     9121 gatcgagagc ggccacgtcg ccggcgcggc gctcgacgtg ttcgccaccg agccggccag
     9181 ggaatcgccg ctcttcgccc tcgatgacgt ggtcgtcacc ccgcatctcg gggcctcgac
     9241 cgccgaggcg caggagaagg tggcgctcga gatcgccgag cagatgagcg actatctgct
     9301 caagggggcc atcgtcaacg cgctgaacat gccctcggtg accgccgagg aggcgccgcg
     9361 cctcaagccc tacatgctgc tcgccgagca gctcggctcg ttcgccgggc agctcaccga
     9421 gaccgggctc gagggggtga ccatccgcta tgccggcgag gtggcgcgcc tgaacacccg
     9481 cccgctcacc cagatgatcc tcaagggcct cctgagcccg ctcctcgcca gcgtcaacat
     9541 ggtgaacgcg ccggcggtcg cgcgctcgcg cgacatcggc gtcaccacgg tcgagaccga
     9601 ggacctcgaa ggctaccaga cgctgatcgc cgtcgaggtc gagaccgagc gcggccggcg
     9661 cgaggtctgc ggcacgctga tccacgacgg ccggccccgg gtcgtccgga tccgcggcat
     9721 cgagatcgag gcccggctcg ccgaccacat gctctatctg cgcaacagcg acacgcccgg
     9781 cttcatcgga gcgctcggcg gcctgctcgg cgatgccggg gtcaacatcg ccaccttcca
     9841 tctcggccgc gaccggccgg gcggcgacgc catcgccatg ctcgaggtcg acggccccgt
     9901 gcccgaggcc gtgctgcgcc ggatccgggc cctgccgcag gtgctgcagg tcaaggcgct
     9961 ggccttttga tcggctcggc ctcgatcgcc tcgatgaacc gggacccata gagctcgcat
    10021 ttcttctggc cgacgccgtc gacggcgagg aacgcttcga ggctggtcgg ccgcttctgc
    10081 gccatgtcga cgagggtcgc gtcgctgaag acgatatagg ccggcttgtc gatctcccgc
    10141 gcgatctcga gccgcagcac cttgagcgcc gcgagcagct ccatcgccgc cggctcgacg
    10201 ggcgcagcgc tggtgcccgc cgccgtcttg cggccgcggc gcgccttggt cggcggggcg
    10261 gtgagatcga gcggcacggc ggcctcgccg cgcagcacgt ccgccgcctt gcgccccggc
    10321 ctgagtgcgc cgtgcccggc gatgtccacc tcgaccacgc ccgatgcata gagctggcgg
    10381 acggcaccgc gcaggacccg cttgtccatg tcggcggcgg cgccgaaggc ggcgagccgg
    10441 tcgtggccga gcgtcgccac cttttcggtg gcctcgccgc ggaggatatc gatcagatgc
    10501 gcctggccga agcgccggcc ggtggcatcg atggcgccga gcagggccct cgcgaggtcg
    10561 cccgcctcga ccagcgccgg cgggttcagg cagcggtcgc aggcaccgca gggcccggcc
    10621 gcctcgccga aatagccgag cagcgtctgc ttgcggcagc cgctcgtctc ggtgaaggag
    10681 agcagcgcgt ccagccgccg gtgctcgacc cgcttgaccg ccgcatcggc attgccgctg
    10741 tcgatcagcc agcggcggcg gcggatgtcg tcgaagccgt ggagcaggat cgcctcggcc
    10801 ggcaacccgt cgcggccggc ccggccgatc tcctggtagt aggcctcgag gttgctcggc
    10861 aggtcgacat gcacgacgaa gcggatgtcc gccttgtcga tgcccatgcc gaaggcgatg
    10921 gtggcgacga cgacgaggtc gggctcggag aggaagcggt cgagcagttg gcgccggaga
    10981 ccggaatcca gcccggcgtg ataggcgacc gcgggaaagc cggcgtcgcg cagggccgcc
    11041 gcggtcgctt cggtgtccct gcgcgcgagg cggtagacga tgccctggcg cccgcgaaag
    11101 ggctcgatca gctcgacgag ctggcgctcg gcgcgctcct tcgccagcac cttgaggctg
    11161 atgttcggcc ggtcgaagcc ctggacgaag acgcgccccg ggcggtgctg gaagagctgg
    11221 ctctcgatgt cggcgcgcgt cgccacgtcc gccgtcgccg tgaagccggc gatgcaggcg
    11281 tccgggtagc gcgccgggag ctggccgaga ttgcggtagt cggggcggaa gtcgtggccc
    11341 cactggctca ggcagtgcac ctcgtcgacg atgatctggc tgagcttcag ccggtcgagg
    11401 gccgcctgca gccgctcgct gccgagcagc tccggcgaga ggtagagcag cttgagcacg
    11461 ccgtccttga tcgactgcca gatgcggccg cgctcgccac ggtcggtgcc gctgtgcacc
    11521 gccgcggcct cgatgccgag cagccggagc tgggccacct ggttgtccat gagggcgatc
    11581 agcggcgaga cgacgaggct gagcccgtcc ttgagcaccg ccggcaactg gtagcagagg
    11641 ctcttgccgc cgccggtcgg catcaccgcg agcaggttct cgccgcgcag caggctctcg
    11701 acgatcgccc gctggccggg acggaactgg cgaaacccga agatctcgtg cagggccgtt
    11761 tcgggatcgg gatggtcggg caagcggatt ccggcgttgg cgaggggcaa ggctctccta
    11821 gcgaaccaca gggcatccgc caagctggca cgccagggcg gtttgcccta aagggctctg
    11881 gttcgtggaa cgatcgtggg ggcggcagcc aaatgaccaa cgtagcggtg gtcggctcgc
    11941 aatggggcga cgagggcaag ggcaagatcg tcgactggct cagcgagcgc gccgacgtcg
    12001 tcgtgcgctt ccagggtggg cacaatgccg gccacacgct ggtcgtgggc ggcgtgacct
    12061 acaagctcag cctgctgccg tccggcgtgg tgcgcaaggg caagctctcg atcatcggca
    12121 acggcgtcgt cgtcgatccc tgggcgctgc tcgaggagat cgagcgcctg cgccagcaag
    12181 gggtggccat cggctccgag accctgcgcg tcgccgagaa cgcgaccctc atcctgccct
    12241 tccacggcga gctcgaccgg gcccgcgagg aggcgcgcga caaggccaat agcggtggcg
    12301 gcaagatcgg cacgaccggc cgcggcatcg gcccggccta cgaggacaag gtggcgcgcc
    12361 gcgcgatccg cgtctgcgac ctcgccgacg aggcggtgct cgagcagaag ctggcggagc
    12421 tcctcctgca ccacaacgcg ctgcgccgcg gcttcggcgt cgacgagatn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cgctcggcga gcgcggccgc gagttcggca
    12601 cggtcaccgg caggaagcgg cgctgcggct ggttcgacgc ggtgctcgtc cgccaggcgg
    12661 tcaagaccgg cggcatccgc ggcctggcgc tcaccaagct cgacgtgctc gacggcttcg
    12721 agcggctgaa gatctgcacc gcctacaacc tcaacggcca gaccctgcac catttgccgg
    12781 ccggcatggc ggcgcaggcg gcggtcaggc cgatctacga ggagatcgag ggctggtcgg
    12841 agagcaccgc cggggccagg agcttcgtcg acctgccggc ccaggcgatc aagtacgtca
    12901 agcgcctcga ggagctggtc gaggcgccgg tggcgctgct ctcgaccagc ccggcgcgcg
    12961 acgacaccat cctggtgacc gaccccttcg ccgactgagc gccggcctcc gggcgcagca
    13021 attccgccac agtcggagcg gcttcgccgg gcggcgaggc gccggcgctt gcctgccgga
    13081 acgaaactct gagactattc gaccagcttg ttgtttgcct tatggtttat aaggaggctg
    13141 gtcatgatca gccgtgccgg tcaggatccg caagtcgctt ttcgcgccgg gctcgcccat
    13201 gcggcgacac cgggcaatgc cgggccgggc gagcgcctgg tgctgcggat gcagcgcctg
    13261 ccggggctgg tcgagccggg tgccgccttc gaggtcggcc gggcgctgcg cgccatcgcc
    13321 cggctcttcg atcgggccgc ggcacccgtc gtcatggtcg agcagttccg tgacaatctc
    13381 gtcggcgccg ccatcgccca gcgtggccgg ctcgagcgca acggccgctg ccggcgcggc
    13441 tggaacctgc aggacacgat cgagatgttc gtcgagatgt acgagcgcct gctcggccag
    13501 tcggccaccg gctatgcgga aatccgcggc tacgtgttcc gggccgcggc cggcccggcg
    13561 cccctgatgc cgctgccggc atcgcgcgtg ccgccccgct tcttccgccg cgccgcctga
    13621 cagcgactgc cgccctcacg cccgaatgaa gccgccgctg cgcagctcgg cgacgaggat
    13681 ccgctgcagc atggtctcgc gcaggctgga tggccggtcg aggccgagcc tcgccagata
    13741 gggcagggcc tcgtccggca cgtccagccc ggcgccgacg gtctccttga cgatgccggc
    13801 gaggccgaga cggcggccca tgcgcgcctt caggcccttc agcacatggc ccagcacctg
    13861 ctcctcatgg gtgagctcgg agccgaaggg gaaggtcggg aaatggccgg cggcgcgcag
    13921 cggcttcagc tccgcttcca gccgggccgg ggtattggca cggtggcggt cggggatgcg
    13981 atgggtcgac gccagcttgc cgctggccac cgcctcggcc cggagatcct cctggaagcg
    14041 cgaatcgctg atgttcaaga gggccgcgat gcactcgtgg tcctgccggc cgcgcaggtc
    14101 ggcgatgccg tactcggtga tcacgatatc gcggaggtgg cgcgggatcg tggtgtggcc
    14161 atagctccag acgatgttcg acgagacctt gccggccttg gtccgcgtgc tgcgcagcat
    14221 gaggatcgag cgcgcgcctt cgagcgcatg cgccatggcg acgaaattgt actggccgcc
    14281 gacgccgctg acgatctgct cgttcgccag cccgtccgag caggccgccc cgcagagcgt
    14341 catcatcatc gccgagttga cgaagcgggc gtggcgccgc tgggccgccg ccagcgcctc
    14401 gtggccgtag agctggttga cgaagccgat gccggtcatg ttgaacttgg cgcgctcgcc
    14461 ctcgtcgagc tcgcgcagca ggtcgtacat cgccctcggg ccgatgaaga agccggcatg
    14521 gaggatcgag ccgccggcga gccggtcgcc caggcattcc gcctcgatcg aggccctggt
    14581 ctcgggatcg gcgaggtcgg gtgagaggcg ccggccgctc ggcgtgacca gttcaccgtc
    14641 ggcgaaggcg atgccctcgc gcaggacgcc gcgccgcacg aggtggtcga ccgcggcggc
    14701 gtcgagcggg ctcgggaccg cacctgcctc gaccagcgca tcgagcgtgc cgagcgtgac
    14761 ctcggcggag atcaggcccg cattgacgag gcgctggatg gccagatcgt catagacctc
    14821 ccggcggagg atgccggccc ggtagaggtg gcgaaagcag tcggcgaaca tctcggtggc
    14881 gccgtagagc ccggtctcga agggggcggt gccgccgatc gcggcgatgc tgtcgccgca
    14941 gcgcccggcg aggccggtgg cccggagcgc ttcctggtag gtggcgttct cccgatggcg
    15001 caggcagagc gcatgggcga tggcgtcgcc gagcgagccg atgccgatct gcaccgtgcc
    15061 gccgtcccgg atcaggctgc tggcggcgag gccgatcagg tagtcggcgg tatggactgg
    15121 gcccgccggc aatgacagca ggtcgtggtc gaggctgcgg tggtcgagca cgaggtcgaa
    15181 ggtcgaggcc gacaccaggg cgtcacggcc catgaagggc aggcgttcgt tgacctgggc
    15241 gacccggacg acccggcgct ccgggtcgct ggcgtccagc aggcggaaga gatcgagcga
    15301 atcgctgttc gagctcaggc tgtaggtcgt ctggccatcc ctgcagcgct tcgccacggc
    15361 ctggacgaag acgttgaccc cctgcagcag caggtcccgg gcgacatggg tgtagttcga
    15421 gctgatatgg tgctgctggg ccgtggcgtt gttcaaaaat gccccggcgc gcatgtagaa
    15481 ctcggtgacc cggatattgg gcggcagcgc gttccggctg taggggccga cataggcgag
    15541 ctccgggcag tcgccgaaca ggcgctcgac gatcggtccc agcatgcgcc tttcgaggtc
    15601 gcttttcgcc tgcggccggg cgagggagag gccggtgacg atccgaagct cgagcgtgcg
    15661 gtcggcggcg gcgcggtggt agagggcgtt cagcagatgc accggcttgc cgaggccgag
    15721 cggggcgccg acgacaatgc ggcggccgac ccgctcgatg atcgcatcga ccatgcgctc
    15781 ggggtcgccg agacgaaggg gcgtgggatc ggacatgccg ctcagaagcc caaggtgagg
    15841 cgctccatca ggtcgacttc ctgctggagg tgggaggctg ccaggacatc ccagctatcc
    15901 caggattcga ccacccggcc gtccttcagg cgatggaagc tcatgccgga tgccgcccgg
    15961 ggcatcgccg gatcggcatc ctccggcagc tccatggtga tggtccagcg gctcgccacg
    16021 agatcgccct cggcgacctg gctctcgatc gagaacacgg cctcgccata gagggcgtgg
    16081 taggtggcga cgaactgccg gtactggtcg atgccctcga gccgcaggcc cgccgacgag
    16141 gcgtcgacga cgcagtcggc cgccaccagc tccggcagca gcgagagatc gccggccccc
    16201 cagacctcct cgagcaaccg gcgaacgacg gccttggtgt cctcagccat gatgtcggca
    16261 tctcctcctt cggcgcggct ctcgacttct agcaagaagg tggcacgggc ggcacgtcag
    16321 tctgaacggc ggcgcagcgc cgccagcgcc gcccgggtcg gggtggtgag cttctccggc
    16381 aggcggtcga gcggaaacca gccgaagccg ccgtgcttga ggggctccat gaggcgcggc
    16441 tcgccggtga actcgcgaca gagaaagacc ggcgccaccc agtggtggcc tgcggcctcg
    16501 tcgatcaggt cgaccatgca gaggaaggtc gggtcgcgga gcacgatgcc gagctcttcc
    16561 gcaacctcgc gccgcgtcgc ctcgaggctc ggctcgagcc agtcgacctt gccgccgggc
    16621 aggccccagt ggccggcctc gggcgggcgc cggcgctcga tcaggaggat cgcgtcgccg
    16681 cgcaggatga cagcaccgca gccgacgccg ggtcgcttat ccgtgacggg gagggtcgtt
    16741 tccaaggttt cggctccata gcgcgaggcg gtgccccggc gcaaacaccg gcgtgccgcc
    16801 ctgcgaccgc gtggcgcccg attccgggcg gaggcggcct tgccggggcc gatatgctgt
    16861 gttaatcgcc acccgtcttt tcgggaacga aacgcacgcc ttgccgcgcg gccctttggc
    16921 gccgtcgtgg ccttcgtgac cagccgggga tcggaattgc ccgccacatc cgccacagcg
    16981 acaggcttgg cccagcgcta cgccacggcc ctgctcgatc tggccaggga agagaagaag
    17041 ctcgacgcgg tcgccgccga cctcaggggc ctcgtcgccc tgctcggcga gagcagggag
    17101 ctgcggcgcc tcgtcgagag cccggtgatc agccgcgagg agcaggggcg ggcgatcctc
    17161 gccgtcgccg acaaggccgg gaccgagccg ctgacggcga agttcctggg cctgctcgcc
    17221 agccgccgcc ggctcttcgc cctgccgggc atcgccaggg cctatgcggc gatgctggcc
    17281 gaggagcgag gcgaggtcgc ggccgaggtg gtctcggcgg tgccgctcgg cgaggccgag
    17341 ctggattccc tgaagaacgc catagccggc catgtcgggc agtcggtgaa cgtggagacg
    17401 cgggtcgatc cggccttgct gggcggggtg gtcgtgcgcg tcggctcgcg catgctcgac
    17461 gcctcgatcc gcaccaagct gcagcatctc gagcagtcga tgagaggaat gggttgatgg
    17521 atattcgcgc cgccgagatt tccgcgatcc tcaaggagca gatcgccaac ttcggcaccg
    17581 aggcggagat ctccgaggtc ggccgggtct tgtccgtcgg tgacggcatc gcccgctgct
    17641 acggcctcga ccaggtccag gccggcgaga tggtcgagtt cgaggacggc acgcgcggca
    17701 tggcgctgaa cctcgaggag gacaatgtcg gcatcgtcat cttcggcgac gaccgcaaca
    17761 tctccgaagg tgcgatcgtc aagcgcacgc agtcgatcgt cgatgcgccg gtcggcaagg
    17821 gcctgctcgg ccgcgtggtc gacggcctcg gcaacccgat cgacggcaag ggcccgatcg
    17881 acgccaccga gcgccgcgtg gtcgacgtca aggcgccggg catcatgccc cggaagtcgg
    17941 tgcacgagcc gatgcagacc ggcctcaagg cgatcgacgc gctga
//
LOCUS       DAOTFI010000092        17893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3784_length_17893_cov_6.797119, whole genome shotgun sequence.
ACCESSION   DAOTFI010000092 DAOTFI010000000
VERSION     DAOTFI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17893
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3784_length_17893_cov_6.797119"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..831
                     /locus_tag="P5558_11030"
     CDS             106..831
                     /locus_tag="P5558_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24887.1"
                     /translation="MWDPRHVLITGASSGIGAALARRLAAPGRRLTLLGRDAPRLAAV
                     AADCAGEVATACVEVQDAPAMAGAIERACAAAPIDLLIANAGISGGDPSTLVAVNVQG
                     VVNTVEPALAPMRARGAGQIALMSSLAGFRGLPNAPAYCASKAAVRLYGEGLRGRLAR
                     DGIGVSVICPGFVRTPLTAANPFPMPFLMEPATAAARIVRGLARNRARIAFPRRLYAL
                     AVLTSLLPAAVVDGLFTRFAAKE"
     gene            837..1511
                     /locus_tag="P5558_11035"
     CDS             837..1511
                     /locus_tag="P5558_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY24888.1"
                     /translation="MQRAPTPRENAAAFVATAELRAALERAAMAVTLAPGDSLFLQGD
                     DADALYLLDQGEIAISVLAPSGRKLGLEIITEGEIFGEIGLFTGRRTASATALGVVRL
                     RQVRRPDLLALIRPEPDLALELIELLCARLRVMSERHEDRAFLPLSTRLARLLLRLQL
                     KIGKADGSLPVSQAELADFTGATREGVAKTLAIWRGQGWIRPGRGTLRILDRAALEAV
                     AASPDD"
     gene            1646..1912
                     /locus_tag="P5558_11040"
     CDS             1646..1912
                     /locus_tag="P5558_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24889.1"
                     /translation="MLNRRRFALHFAMTAALGLTPLAAAPLARAEESTILPDTYRGSP
                     RRRGGRGRPRRSTAAAAGTATPGPRAKSREQLELRPGTALTATE"
     gene            complement(1946..2935)
                     /gene="mltG"
                     /locus_tag="P5558_11045"
     CDS             complement(1946..2935)
                     /gene="mltG"
                     /locus_tag="P5558_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRY24890.1"
                     /translation="MTWLRRLAAAALGLLLLAVLAAGAGWWLLGRYMETPGPLAQEQV
                     IELERGSGLAAIARRLEDAGVIDDARVFRLAARLTGRDRKLRAGEYRVPAAVSPRELL
                     DLLESGQVILHALTVPEGLTTSEALAVVAADPVLTGPMPEEPPGEGTLLPETYHFPRG
                     TTRIAIVEQMQVDMSRLLAELWAGRQADLPIETPEEALILASIIEKETAVPDEYGLVA
                     GVFVNRLRRGMLLQTDPTVIYALTRGEGPLGRQLLRRDLEVDDPYNTYVHAGLPPGPI
                     ANPGRGALAAAVNPAETDYLYFVADGTGGHAFAKSLAEHNRNVAKWRRVRDGG"
     gene            complement(2949..4217)
                     /gene="fabF"
                     /locus_tag="P5558_11050"
     CDS             complement(2949..4217)
                     /gene="fabF"
                     /locus_tag="P5558_11050"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRY24891.1"
                     /translation="MRRVVVTGIGLVSPLGGDVATSWANLVAGKSGIGRTTGFEIDDL
                     PSKVAGHVPRGEGPGLFNATDYVEAKDLRKNDPFIVFGIAAAAQAMADCGYRPETEEQ
                     QERAGVQIGSGIGGLQTIAETTLILEKSGPRRVSPFFIPASLINLVSGQVSIRHGLKG
                     PNHSAVTACSTGAHAIGDAARFIALDDADVMVAGGAEAAICRLGIAGFCAARALSTGF
                     NDTPERASRPWDRDRDGFVMGEGAGVVVLEELEHAKRRGARIYAELKGYGVSGDAHHV
                     TAPAPEHAGAFRSMRNALRRAGLEPSDIDYVNAHGTSTPLGDELEFGAVKRLFGPAAA
                     NVKMSSTKSAIGHLLGAAGSAEAIFSILAMRDGVVPPTLNLDNPSDDCQGIDLVPHAA
                     KEMKVRAALSNSFGFGGTNATLVFAHPDTI"
     gene            complement(4291..4527)
                     /locus_tag="P5558_11055"
     CDS             complement(4291..4527)
                     /locus_tag="P5558_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY24892.1"
                     /translation="MSDIAERVKKIVIEHLGVEEAKVTDTASFVDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAEKIATVQDAINYIEANAS"
     gene            complement(4755..5480)
                     /gene="fabG"
                     /locus_tag="P5558_11060"
     CDS             complement(4755..5480)
                     /gene="fabG"
                     /locus_tag="P5558_11060"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRY24893.1"
                     /translation="MFDLTGRTALVTGASGGIGGAIARALHAQGAKVVLAGRREAPLA
                     ALAAELGERAGVLVGDLADPAGLAALGAAAAEADILVNNAGITRDQLAMRMKDDDWQA
                     VIDTNLGAAFRLSRAALKGMMKRRHGRIVNITSVVGVTGNPGQTNYAAAKAGLIGMSK
                     ALAQEVASRNITVNCVAPGFIETAMTDGLDARQKELLLGRIPMARLGQGMDVAAAVAY
                     LASDEAAYVTGQTLHVNGGMAMP"
     gene            complement(5491..6447)
                     /gene="fabD"
                     /locus_tag="P5558_11065"
     CDS             complement(5491..6447)
                     /gene="fabD"
                     /locus_tag="P5558_11065"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRY24894.1"
                     /translation="MTRIAVFPGQGAQAVGMGRALFDAEPAARAVFEEVDEALAEKLS
                     TLIFEGPEEALTLTRNAQPALMATSLAAVRALEARAARKLGDMVDQAAGHSLGEYSAL
                     AAMGALDVGTAARLLRLRGEAMQAAVPAGDGAMAAVLGLAPDAALEVAAAAEAATGGV
                     CELANDNADGQAVLSGDRTAVEAACALAKEKGAKRAMLLQVSAPFHCRLMGPAAERLA
                     EALGQTAFKAPARPVIANVTAEPVSDPALLPGLLTRQVTARVRWRETMQRLAAGDTKL
                     IAELGAGKVLTGLARRAVPTAALFNVQGPDDLDGLVQALQTS"
     gene            6613..7077
                     /locus_tag="P5558_11070"
     CDS             6613..7077
                     /locus_tag="P5558_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24895.1"
                     /translation="MARGAGTASIPLLFVDLSPAGDRPLPDAPGADHVQGRALVPPVE
                     AAPDRLAGDGDDPFGVDLLAECRHEALAARLEAGWIEHAEQAPEGAMARNTAIQPRDR
                     QNPQKIVPLRIAAAGMRQSRETGAKPIHRLHVRFPWVRFPWVRFPWVRFPWL"
     gene            7159..8124
                     /locus_tag="P5558_11075"
     CDS             7159..8124
                     /locus_tag="P5558_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY24896.1"
                     /translation="MSLRLLLATAAVAGFAASASAQTVIEVGYATTTDSHYGMGMTRF
                     GEVLEDLSGGEMTLVQHPAAALGGEREMIEAVQIGTLDMVITSSGPLPNFVPETQVLD
                     LPFLFRDYDHARGVLDGEIGQELLAKIDEAGFKALAWTENGFRHITNSRQPVKTPADL
                     AGLKIRTMENPIHLKAFEALGAAPTPMSFAELFTALQQGVVDAQENPIVVISVSNFAE
                     VQDYLTLSGHLYSPAIILMSDALWSGLSEEEQGWIMEAAAASVEVTRGRVSELEENGV
                     QGLIDKGMEVVTEIDRPAFEAAVQPAYTAYTDQYGDELIERIRAQ"
     gene            8143..8706
                     /locus_tag="P5558_11080"
     CDS             8143..8706
                     /locus_tag="P5558_11080"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY24897.1"
                     /translation="MASARPPAGGTPWRRPAWLRAIRAVERGLTEAALALAMVGVALM
                     ASFTCYQVFMRFVLGQPSTWSEVLVRSIMIWTVYLAAAAAFREGAMIAAEVLVRAVPR
                     QLGRALQIFAGALSLLFLVILVWTGIDMVMRTQSQTLAGLEIPIFWIYLALPVGGCLA
                     ALAVVVRTGELLEPGATIHEAHLDTIA"
     gene            8729..10003
                     /locus_tag="P5558_11085"
     CDS             8729..10003
                     /locus_tag="P5558_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY24898.1"
                     /translation="MNAAIMLTLIVLIALGVPVAISLALASIIGIEAFTRLPLILVAQ
                     RMLTGIDSFPLMAVPLFILAGNLMAAGGISRRLVDFARALVGGIQGGLAASCVVTCMI
                     FAAVSGSSVATTFAIGAILIPAMVQHGYPAPTAAAVQAASAELGVLIPPSIPLILYGV
                     ATDTSIGQLFIAGFGPGILVAGSLIGFLLVYCRVKGYGNEDGAEREALGPAAVGASAA
                     LIMPVIVVGGIYAGVFTPTEASGIAVFYSLVVGAFVYRELTWPKLRDVLRETAIATAV
                     VMLIIAAASLFSFLLSRTGLPAMATTWVEATFTSRFTFLLAVNAFLFLVGMFIETAAA
                     ILVLAPILVPIAIGFGVDPVHFGLIMVINLAMGMITPPVGVNLFAACAVARLPIERIV
                     PALLPFFAVIVACVMAVTYLPWISLGLRDLLY"
     gene            complement(10074..10535)
                     /locus_tag="P5558_11090"
     CDS             complement(10074..10535)
                     /locus_tag="P5558_11090"
                     /inference="COORDINATES: protein motif:HMM:NF022159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HRY24899.1"
                     /translation="MRQSIDDGRACTHGWCRGIGRFAVSAAMLAQLASMPAVAQSLND
                     LTGLLGKSGGGLGLPMVDQAGPANVAGVLEYCVKNNYLGGGDAASVQQSLLGKLGGTE
                     EAEKDPGYEAGLGGLLDTGGGDTFSLGGAGLQETVTEQVCDLVLEHGQSFL"
     gene            complement(10665..12470)
                     /locus_tag="P5558_11095"
     CDS             complement(10665..12470)
                     /locus_tag="P5558_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY24900.1"
                     /translation="MTLGTDLLEALPVAVYATDAQGRVTFRNEAAVTLWGERPALGSR
                     IDDGSWHLLWPDGRAMRPEETPLAVVLRDGQAWSGRAVILVREDGARQALAAHAGPLR
                     DGSGKLTGAVMLLADSGDTRQSEIAALHLAAIVASSDDAIVSKTLDGIVTSWNAGAVR
                     IFGYTQEEMIGRSIKRIIPADLQQEEDEILARLRAGERIDHFDTVRVAKDGRQVHISL
                     TISPIRDSSGSIVGASKVARDVTERKRSEEMRALLFSELNHRVKNTLATIQAIASQSM
                     RSTTSPEAFVAAFNGRVQALARAHDLLVQGGMRGATLGDLVREQVLLGSGDGARIVTS
                     GPRVVLDARVAVQLALVLHELATNARKHGALAVPGGRLSITWEVAVAAGRELHLTWRE
                     SGVPGLEVPGRKGFGSTLIERSLEANRGEARVSYAGDGLACAIRLPLAETFDNLADAR
                     LSSALSPPPAPRPECPVAAARKGRVLVVEDEPLVALAIEEELRAVGYTVLGPVASAAR
                     AIPLIAEPGCEAALVDANLDGQPVDDIVAALTERGVPFAFATGYGREALPRPFRDAPL
                     LTKPFSHSQLLAIVEQLLAGHRATSTGSDLPPARP"
     gene            12649..13263
                     /locus_tag="P5558_11100"
     CDS             12649..13263
                     /locus_tag="P5558_11100"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY24901.1"
                     /translation="MLIRKPAATRKAEIVEAALRLADELGPDRLTTEAIAGAVGLTQP
                     AIFRHFPTKQDLWEAVGARIGELMEERWAAVPADEGRATDRVRALVATQLGLIQATPA
                     IPAILFSRELHVENQGLRRAFLELMSRFHRLIAGLLAQARDDGELCPELDPDDLAALV
                     IGLPPGLAVRWSLSDRGFDLGEEGRRLLELQLRGFTSEATRRSS"
     gene            13260..14423
                     /locus_tag="P5558_11105"
     CDS             13260..14423
                     /locus_tag="P5558_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY24902.1"
                     /translation="MSSRLVRLLLALLALGATALAIFLFVFMRPLPVEVARPASQVPI
                     EVFGLGTVEARILSEIGFEVGAALVELNADHGDRVRQGDILARLHGAEQEARVARARA
                     GVVNAEAAVQRMDAAVGRAEAVLAQRAQTNRRRRELVARQAISIETAEEAEMEEAVAR
                     AELAVASSDVDVAEAALDDARAQLQMEQVLLEHHVLKAPYDAIVVARHRELGYVLDPG
                     EPLFTLVAPETVWTLAYVDEARAGGLRVGQPALVHLRSRPHEMLQGHVTRIDIESDRV
                     SEERRVYVACDQCPESFHLGEQAEVFITTTVLESALLVPEIAVEHFDGTRGTVWTVED
                     GVLHRRAVTFGERTLDSRLAVSDGLPAGAEVVSTLRPGLREGRRARVTEAGAP"
     gene            14420..15553
                     /locus_tag="P5558_11110"
     CDS             14420..15553
                     /locus_tag="P5558_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24903.1"
                     /translation="MNLAYRDVRHNLGRFLLTCFGLSLLLGVVLSMIGIYRGLVEDAL
                     TLVRTPAVDVWVVESGTRGPFAEASRIPGDTREAIARLTGVTEAGSVTYQSVETLLMG
                     TKLRLYVVGYEPGRPGGPDRITAGRPIGRSHYEMVADRRSGLALGDRLPLGRNTFTVV
                     GLTDGQVSSGGDPVVYLTLLDSQKLQFELAPPAARRELARGGGQGGTDTINAVVARLS
                     PNASPENAANATRRWKHLSAMTQDGQETILTRSVVERARKQIGLFTTLLLIVSAVIIA
                     LILYTMTMDKIREIATLKLIGAADRTIIGLILQQALAMGAIGFGFGALLITTVKDYFP
                     RRVVLQPEDGLALGAAVLVLCVLSSALGVRLALRVDPANALGG"
     gene            15556..16263
                     /locus_tag="P5558_11115"
     CDS             15556..16263
                     /locus_tag="P5558_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24904.1"
                     /translation="MQAAAGNRPLVRVAHVSKHFGAGETRVDALRDVSLEVQPGQVVA
                     LLGPSGSGKTTLLNVIGCITEPSSGRLELDGEAVYDGRWLRGDLRKLRLEKIGFIFQF
                     HNLLPFLSSTDNVAVVLSLAGLGTAAARRRAVELLDYLEVGHRRDAMPAKLSGGEAQR
                     VAIARALANRPRIILADEPTAALDSERARIVMDLLRKLATEQQAAILAVTHDEKIFDR
                     FDHIFHLRDGRLDNQEP"
     gene            16504..17442
                     /locus_tag="P5558_11120"
     CDS             16504..17442
                     /locus_tag="P5558_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24905.1"
                     /translation="MRPCVFIHTNHKQYVGALVSAYSFRRFAKDPDAFDVQIIHTKDF
                     PFLREHEGEAYLRDGVKRHWRYDDLQSFTTLRFMPPERMGYEGRSVVVDPDVFAVGDV
                     GELLARDMGGKAIIARTRPGPRTLTDGGRYNSSVMLLDNAKLRHWRVEEQFEAMFEMQ
                     RDYRRWITLGYEDSATIGELEREWNDLDHLDTRTRMIHNTHRKTQPWKSGLPVDFIPA
                     ERFRPFPPIAWALRLRRKLFGDYGLLGTYKPHPDRNQEKLFFGLLKECLANGLVTEEL
                     VREEMRQNHVRKDALDVIAAAPPLSSLPLSALPRAA"
     gene            complement(17514..>17893)
                     /locus_tag="P5558_11125"
     CDS             complement(17514..>17893)
                     /locus_tag="P5558_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24906.1"
                     /translation="GDGAGTGNVGIMSGNGNAGENSGRDNVGILVGNDNSGDFSGRES
                     IGVLVANGNAGDASGRENVGIVVGNGNAGNGSGRENIGVLVGNGNEGGGSGRENLGIL
                     LGNGNLAGGAGRELIGILLGNYD"
BASE COUNT         2456 a         6585 c         6119 g         2733 t
ORIGIN      
        1 ggggacgaca tgaccagcat caggccgcgc cggcggccgg gtatcagacg gtcgagcgcg
       61 atcatgcatg caatcctgga gaaggccggc tggagaagcg ctatggtgtg ggatccgcga
      121 catgtgctga tcaccggtgc aagtagcggc attggcgcgg cactggcaag acggctggcg
      181 gcaccggggc ggcggctcac cctcctcggc cgcgatgcgc cgcgcctcgc ggcggtcgcc
      241 gccgactgcg ccggcgaggt cgcgaccgcg tgcgtcgagg tccaggacgc cccggccatg
      301 gccggggcca tcgagcgcgc ctgcgcggca gcgcccatcg acctcctgat cgccaatgcc
      361 ggcatttccg gcggcgatcc ctcgaccctc gtggcggtca acgtccaggg cgtcgtcaac
      421 acggtcgagc cggccctggc gcccatgcgc gccaggggtg ccggccagat tgcgctcatg
      481 agctcgctcg ccggcttccg cggcctcccg aacgcgcccg cctactgcgc cagcaaggcc
      541 gccgtccgcc tctatggcga gggcctgcgc ggccggctcg ccagggacgg catcggcgtc
      601 agcgtcatct gcccgggctt cgtcaggacc ccgctcacgg cggccaaccc cttcccgatg
      661 ccgttcctga tggagccggc gaccgccgcc gcccggatcg tccggggcct cgcccgcaac
      721 cgggcacgga tcgccttccc gcgccgcctc tatgccctgg ccgtgctcac cagcctgttg
      781 ccggcagccg tggtcgatgg tctcttcacc cggttcgcgg ccaaggagtg accgccatgc
      841 aaagggcgcc gacgccccgc gagaacgcgg cggccttcgt ggcgaccgcc gagctgcgcg
      901 ccgccctcga gcgggcggca atggccgtga cgctggcccc cggcgacagc ctcttcctgc
      961 agggcgacga cgccgacgcc ctctatctgc tcgaccaggg cgagatcgcg atcagcgtgc
     1021 tggcgccgag cggccgcaag ctcgggctcg agatcatcac cgagggcgag atcttcggcg
     1081 agatcgggct cttcaccggc cggcggaccg ccagcgccac ggcgctgggt gtcgtccggc
     1141 tgcgccaggt gcgccggccc gatctcctgg cgctgatccg cccggagccg gatctggcgc
     1201 tggagctgat cgagctgctc tgcgcccggc tgcgggtcat gagcgagcgg cacgaggatc
     1261 gcgccttcct gccgctctcg acccgcctcg cccggctgct gctgcggctc cagctcaaga
     1321 tcggcaaggc ggacggcagc ctgccggtgt cgcaggccga gctggccgac ttcaccggcg
     1381 cgacgcggga aggcgtcgcc aagaccctgg cgatctggcg cggccagggg tggataaggc
     1441 ccgggcgcgg cacgctccgc atcctcgacc gggcggcgct ggaagccgtg gcggcaagcc
     1501 cggacgacta attctgcccc accgtgtgaa cccgttcaca gagccgccgg ccgagccggg
     1561 gcatggtcac cgtgccggcc gggtcgatca cccggggcca cccggcccgt tggctggacc
     1621 ctaccaaccg tggaggaaga cgatcatgct caaccgccgt cggttcgccc tgcattttgc
     1681 catgactgcc gccctgggcc tgacccctct ggcagcggcg ccgctcgccc gggccgagga
     1741 gtcgaccatc ctccccgaca cgtatcgcgg gtcgccccgc agacgcggtg gtcgcggacg
     1801 gccccgccgc agcaccgcgg ccgctgccgg aacggcgacg cccgggccgc gggccaagtc
     1861 ccgcgagcag ctggagcttc ggcccggcac ggccctcacc gcgaccgagt aggccggccc
     1921 ggcggggcgg gcgagcggct gcccttcagc cgccgtcccg cacgcgccgc cacttggcga
     1981 cgttgcggtt gtgctcggcc aggctcttgg cgaaggcatg gccgccggtg ccgtcggcga
     2041 cgaaatagag atagtcggtc tcggccgggt tcactgcggc cgcgagggca ccgcgcccgg
     2101 gattggcgat cggccccggc ggcagcccgg catggacata ggtgttgtag gggtcgtcga
     2161 cctcgaggtc gcggcgcagg agctgccggc cgagggggcc ctcgcccctg gtcagcgcgt
     2221 agatcacggt cgggtcggtc tgcagcagca tgccgcggcg caggcggttg acgaagacgc
     2281 cggcgacgag cccgtactcg tccggcaccg ccgtctcctt ctcgatgatc gaggcgagga
     2341 tcagcgcctc ttcgggcgtc tcgatgggca ggtcggcctg ccgcccggcc cagagctcgg
     2401 cgaggaggcg gctcatgtcg acctgcatct gctcgacgat ggcgatgcgc gtggtgccgc
     2461 gcgggaagtg ataggtctcg ggcagcagcg tgccctcgcc cggcggctcc tcgggcatgg
     2521 gcccggtgag gaccgggtcg gccgcgacca ccgccagcgc ctcgctggtg gtcagcccct
     2581 ccggcacggt cagcgcatgc aggatgacct gcccgctctc caggagatcg agcagctccc
     2641 ggggactgac cgccgccggc acccggtact cgccggccct gagcttgcgg tcgcgaccgg
     2701 tcagccgggc ggcgaggcga aagaccctgg catcgtcgat caccccggcg tcctcgagcc
     2761 gcctggcgat ggcggcgagg ccgctgccgc gctcgagctc gatcacctgc tcctgggcga
     2821 gcgggccggg cgtctccatg tagcggccga gcagccacca gccggcgccc gccgcgagca
     2881 cggcgagcag caggaggccg agggcggcgg cagcgaggcg gcgcagccag gtcacgggcg
     2941 gcggctgctc agatcgtgtc ggggtgggcg aagaccaggg tcgcgttggt gccgccgaag
     3001 ccgaaggagt tggagagggc ggcccgcacc ttcatctcct tggcggcgtg cggcacgagg
     3061 tcgatgcctt ggcagtcgtc cgacgggttg tcgagattga gcgtcggcgg caccaccccg
     3121 tcgcgcatgg cgaggatcga gaagatcgcc tcggcgctgc cggcggcccc cagcagatgg
     3181 ccgatcgccg acttggtcga cgacatcttc acgttggccg cggccgggcc gaagaggcgc
     3241 ttgacggcgc cgaactcgag ctcgtcgccg agcggcgtcg aggtgccgtg cgcgttgacg
     3301 tagtcgatgt cggagggctc cagcccggcc cggcgaagcg cattgcgcat cgagcggaag
     3361 gcgccggcat gctcgggtgc cggggcggtg acgtggtggg cgtcgccgga gacgccgtag
     3421 cccttgagct cggcgtagat ccgagccccg cgccgcttcg cgtgctcgag ctcctcgagc
     3481 acgacgacgc cggccccctc gcccatgacg aagccgtccc ggtcccgatc ccatggccgg
     3541 gacgcgcgct cgggcgtgtc gttgaagccg gtcgagaggg cgcgggcggc gcagaacccg
     3601 gcgatgccga gccggcagat cgcggcctcc gccccgccgg cgaccatcac atccgcgtcg
     3661 tcgagggcga tgaagcgggc ggcatcgccg atcgcgtgcg ctccggtcga gcaggcggtg
     3721 accgccgagt ggttcggccc cttgagcccg tggcggatcg agacctggcc gctgaccagg
     3781 ttgatgagcg atgccgggat gaagaagggg ctgacccgcc gggggccgct cttctccagg
     3841 atcagcgtcg tctcggcgat ggtctgcagc ccgccgatgc cggagccgat ctgcaccccg
     3901 gcccgctcct gctgctcctc ggtctcgggc cggtagccgc aatccgccat cgcctgggcg
     3961 gcggcggcga tgccgaagac gatgaacggg tcgttcttgc ggaggtcctt ggcctcgaca
     4021 tagtcggtgg cgttgaagag gccgggcccc tcgccacgcg gcacatggcc cgccaccttc
     4081 gatggcaggt catcgatctc gaagccggtg gtccggccga tgcccgactt gccggccacc
     4141 agattggccc atgaggtcgc gacatcgccg ccgagcggcg agacgagccc gataccggtg
     4201 acgacgacac gacgcatcag ctcagctcca caactgccgg ccgctggccg gccggagccg
     4261 gagcctcggg ccggacctgc cgcccgacat tcaggatgcg ttggcctcga tgtagttgat
     4321 cgcgtcctgg acggtggcga tcttctcggc cgcatcgtcc gggatctcgc agccgaactc
     4381 ctcctcgaag gccatgacca gctcgaccgt gtcgaggctg tcggcgccga gatcgtcgac
     4441 gaagctcgcc gtgtcggtga ccttcgcctc ctcgacaccg aggtgctcga tgacgatctt
     4501 cttcacgcgt tccgcgatgt cgctcatgtg tgtcctgctc cgcatggccg ggccctgggc
     4561 cgtcggcgtc gccgaggtgc cgggcaccct cggacgttct ggtttcacca ccggtggttg
     4621 gcgcttctgc cggttttggc tccagctggc aaccgcgccc gccctttagc cgaccggtgc
     4681 agccctgact aggcgaagca cggccgcccg gtcaatcacc ctgccggcgt tgccggttcg
     4741 acgcggtgcc ccgctcaggg catcgccatc ccgccattga cgtgcagggt ctgccccgtg
     4801 acgtaggccg cctcgtcgct ggcgagatag gcgaccgcgg ccgcgacatc catgccctgg
     4861 ccgagccgcg ccatcgggat gcggccgagc agcagctcct tctgcctcgc gtcgagcccg
     4921 tcggtcatcg ccgtctcgat gaagcccggg gcgacgcagt tcacggtgat gttgcggctc
     4981 gccacctcct gcgccagggc cttgctcatg ccgatcagcc cggccttggc cgcggcatag
     5041 ttggtctggc ccgggttgcc ggtgacgccg accaccgagg tgatgttgac gatgcgcccg
     5101 tggcgccgct tcatcatgcc cttcagcgcc gcccgggaaa ggcgaaaggc ggcgccgaga
     5161 ttggtgtcga tcaccgcctg ccagtcatcg tccttcatcc gcatggcgag ctggtcgcgg
     5221 gtgatgccgg cattgttgac gaggatgtcg gcctcggccg ccgccgcgcc gagcgccgcc
     5281 aggcccgccg ggtcggcgag atcgccgacc agcaccccgg cccgctcgcc gagctcggcc
     5341 gccagcgcgg cgagcggcgc ctcgcgccgg ccggcgagga cgaccttcgc cccctgggcg
     5401 tgcagcgcgc gggcgatcgc cccgccgatg ccgcccgagg cgccggtcac cagggccgtc
     5461 cggcccgtca gatcgaacat cgcagctcct tcagctcgtc tgcagcgcct ggacgagccc
     5521 gtcgaggtcg tccggcccct gcacattgaa gagcgccgcc gtcggcaccg cccgccgggc
     5581 gaggccggtc agcaccttgc cggcgccgag ctcggcgatc agcttcgtgt cgcccgcggc
     5641 gagtcgctgc atggtctcgc gccagcgcac ccgcgcggtc acctggcggg tcaggagacc
     5701 cggcagcagc gccgggtcgc tcaccggctc ggccgtgaca ttggcgatca ccggccttgc
     5761 cggcgccttg aatgcagtct gccccagcgc ctcggccagc cgctcggccg ccgggcccat
     5821 cagccggcag tggaaggggg cggagacctg gagcagcatc gcccgcttcg cccccttctc
     5881 cttcgccagg gcgcaggccg cctcgaccgc tgtccggtcg ccggagagca cggcctgacc
     5941 atccgcattg tcgttggcga gctcgcagac cccgccggtc gcggcctcgg cggcggcggc
     6001 cacctcgagc gccgcatcgg gggcgaggcc caagacggcc gccatcgccc cgtcccccgc
     6061 gggcacggcc gcctgcatcg cctcgccgcg gagccggagc agccgtgctg ccgtgccgac
     6121 gtcgagggca cccatggcgg ccagggccga atactcgccg agcgaatgcc cggccgcctg
     6181 gtcgaccatg tcgccgagct tccttgcggc ccgcgcctcg agcgcccgca ccgcggcgag
     6241 cgacgtcgcc atcagcgccg gctgggcgtt gcgggtcagt gtcagcgctt cctcggggcc
     6301 ctcgaagatc agcgtcgaga gcttctcggc caaggcctcg tcgacctcct cgaacaccgc
     6361 ccgggcggcc ggctcggcat cgaagagggc ccggcccatg ccgacggcct gcgccccctg
     6421 tcccggaaat accgcgatcc gcgtcatcat tgccgacttg tcgttgtgag cagaagagtc
     6481 cgccgtcggt gtcacgcagg cgccaggctg tcaaggcagg cggaccgggc acccggcgcg
     6541 atcgcatcag gcagctctcg atctgccagt gggcgcgggc gatcctggcg aggcgggtgg
     6601 cggtgagcga gcatggccag gggcgccggc acggcctcga tcccgttgct cttcgtcgac
     6661 ctttctccgg ccggggaccg gccgctgccc gacgctccag gcgccgacca tgtgcagggc
     6721 cgagcgctcg ttccgcctgt cgaagcggcg cccgatcgtc ttgccggcga cggcgatgat
     6781 cccttcggcg tcgatctgct cgccgaatgc cggcacgaag cgctggcagc acgtctcgaa
     6841 gccgggtgga tcgagcatgc ggaacaggcg ccggaaggtg ccatggctcg gaataccgcc
     6901 atccagccgc gcgatcggca aaatccccag aagatcgtcc ccttgcgcat tgccgcggcg
     6961 gggatgcgcc agagccgcga aacaggtgcg aaaccgatcc atcgtctcca tgtgcggttc
     7021 ccctgggtgc ggttcccctg ggtgcggttc ccctgggtgc ggttcccctg gctttgaccg
     7081 cgcccggccg ggcgtgctaa tcgccgttcg gtatcgtccc gcaatgacgg gcagtcggat
     7141 ccacgaggga ggaacaagat gagcctgcgc ctgctgctcg ccacggccgc ggtggccgga
     7201 ttcgccgcat ccgcttcggc ccagaccgtg atcgaggtcg gctacgccac caccaccgat
     7261 tcccattacg gcatgggcat gacccgcttc ggcgaggttc tggaggatct ctccggcggc
     7321 gagatgaccc tggtccagca cccggcagcg gcgctcggcg gcgagcgcga gatgatcgag
     7381 gcggtgcaga tcggcaccct cgacatggtc atcacctcgt ccgggcccct gcccaatttc
     7441 gtccccgaga cccaggtcct cgacctgccc ttcctctttc gcgactacga ccacgcgcgg
     7501 ggcgtgctcg acggcgagat cggccaggag ctgctcgcca agatcgacga ggcgggcttc
     7561 aaggcgctgg cctggaccga gaacggcttt cgccacatca ccaactcgcg ccagccggtg
     7621 aagacgccgg ccgatctcgc cgggctgaag atccgcacca tggagaaccc gatccacctc
     7681 aaggcgttcg aggcgctggg tgccgcaccc acgccgatga gcttcgccga gctcttcacc
     7741 gccctgcagc agggtgtggt cgacgcccag gagaatccga tcgtcgtcat ctcggtgagc
     7801 aacttcgccg aggtgcagga ctacctcacg ctctccggcc acctctactc gccggccatc
     7861 atcctcatgt ccgatgcgct ctggtcgggc ctctccgagg aagagcaggg ctggatcatg
     7921 gaagccgctg cggcctcggt cgaggtgaca cgcggccggg tcagcgagct cgaggagaac
     7981 ggtgtgcagg gcctgatcga caagggcatg gaggtcgtga ccgagatcga ccggccggcc
     8041 ttcgaggcgg cggtgcagcc ggcctacacc gcctacaccg accagtatgg cgacgagctg
     8101 atcgagcgca tccgggcgca gtaggcaccg gggccggcca gcatggcatc ggcgcggccg
     8161 ccggctggcg ggacgccctg gcggcgcccc gcctggctcc gcgcgatccg ggccgtcgag
     8221 cgcgggctga ccgaggcggc gctcgccctc gccatggtgg gcgtggcgct gatggccagc
     8281 ttcacctgct accaggtctt catgcgcttc gtcctgggcc agccctcgac ctggtccgag
     8341 gtgctggtcc gctcgatcat gatctggacg gtctacctcg ccgccgccgc cgcctttcgc
     8401 gagggtgcca tgatcgcggc cgaggtcctg gtgcgcgccg tgccgcgcca gctcggccgg
     8461 gctctccaga tcttcgcggg cgcgctatcg ctgctcttcc tcgtcatcct ggtctggacc
     8521 gggatcgaca tggtcatgcg cacccagagc cagaccctgg ccgggctcga gatccccatc
     8581 ttctggatct acctggcgct gccggtcggc ggctgcctcg ccgcgctcgc ggtggtggtc
     8641 cgcaccggcg agctgctgga gcccggggcc accatccacg aggcgcatct ggacacgatc
     8701 gcatgatgcc ggccggggga agccgcccgt gaacgccgcc atcatgctga cgctgattgt
     8761 cctgatcgcc ctcggcgtgc cggtggcgat cagcctcgcc ctcgcctcga tcatcggcat
     8821 cgaggccttc acccgcctgc ccctgatcct ggtggcgcag cgcatgctga ccgggatcga
     8881 cagctttccc ctgatggcgg tgccgctctt catcctcgcc ggcaacctga tggcggcggg
     8941 cggcatctcg aggcgcctcg tcgacttcgc ccgggccctg gtcggcggca tccagggcgg
     9001 cctcgccgcg tcctgcgtcg tcacctgcat gatcttcgcc gccgtctccg gctcgtcggt
     9061 ggccaccacc ttcgccatcg gcgccatcct gatcccggcc atggtgcagc acggctaccc
     9121 ggcgcccacc gccgccgcgg tccaggcggc cagcgccgag ctcggcgtgc tgatcccgcc
     9181 ctcgatcccg ctcatcctct atggcgtcgc caccgacacc tcgatcggcc agctcttcat
     9241 cgccggcttc ggcccgggca tcctggtggc cggcagcctg atcggcttcc tcctcgtcta
     9301 ttgccgcgtc aagggctacg gcaacgagga cggggccgag cgcgaggccc tgggccccgc
     9361 cgcggtcggc gcctcggcgg cgctgatcat gccggtcatc gtcgtcggcg gcatctatgc
     9421 cggcgtcttc acgccgaccg aggcctcggg catcgccgtc ttctattcgc tggtggtcgg
     9481 cgccttcgtc tatcgcgagc tgacctggcc gaagctcagg gacgtgctcc gcgagaccgc
     9541 catcgccacc gcggtcgtca tgctgatcat cgccgccgcc tcgctcttct cgttcctgct
     9601 ctcgcgcacc ggcctgccgg ccatggcgac cacctgggtc gaggccacct tcacctcgcg
     9661 cttcaccttc ctgctcgcgg tcaacgcctt cctcttcctc gtcggcatgt tcatcgagac
     9721 cgccgccgcc attctcgtcc tcgccccgat cctcgtcccc atcgccatcg gcttcggcgt
     9781 cgatcccgtg catttcgggc tgatcatggt gatcaacctc gccatgggca tgatcacccc
     9841 gccggtgggc gtcaatctct tcgccgcctg cgccgtcgcc cgcctgccga tcgagcgcat
     9901 cgtgccggcc ctgctgccgt tcttcgccgt catcgtcgcc tgcgtgatgg ccgtgaccta
     9961 cctgccctgg atctcgctcg gcctgcgcga cctgctgtac tgacggggcc ggcctctcca
    10021 cccaggagct ggtgccggcg cggtgagcga cgccgcccgg gccgcgccgc gtgtcagagg
    10081 aacgactgcc cgtgctccag cacgaggtcg cacacctgct cggtcacggt ctcctggagg
    10141 ccggcgccgc cgaggctgaa ggtgtcgccg ccgcccgtgt cgagcaggcc gccgaggccg
    10201 gcctcgtagc cagggtcctt ttcagcctct tcggtgccgc cgagcttgcc gagcagcgac
    10261 tgctgcaccg acgcggcgtc gccgccgccg aggtagttgt tcttcacgca atattcgagc
    10321 acgcccgcga cattggccgg gccggcctga tcgaccatcg gcaggccgag cccgccgccg
    10381 ctcttgccga ggaggccggt caggtcgttc agcgactgcg ccaccgcagg catggaagcg
    10441 agctgcgcca gcatggcggc gctgaccgcg aagcggccga tgccccggca ccagccgtgc
    10501 gtgcacgcgc ggccgtcgtc gatgctctgc ctcaagatcc cactcccgtt gttcgtgtcg
    10561 catcgccgga cagcgaagcg ctcataccag acatagcatc gtgacgcccc ctggccagcg
    10621 attgccctcc ccgcccgagg ccgcctgccg ctcccttttc cggctcaggg ccgtgcgggc
    10681 ggcaggtcag accctgtgga cgtggcccgg tgcccggcga ggagctgctc gacgatcgcc
    10741 aggagctggc tgtggctgaa gggcttggtc aggagcggtg catcccggaa gggccgcggc
    10801 agcgcctcgc ggccgtagcc cgtggcgaag gcgaagggca cgccccgctc ggtcagcgcc
    10861 gccacgatgt cgtcgaccgg ctggccgtcc agattggcgt ccaccagcgc cgcctcgcag
    10921 ccgggctcgg cgatcagcgg aatggctcgc gcggcgctgg cgacggggcc cagcaccgtg
    10981 tagcccaccg ccctgagctc ctcctcgatg gcgagcgcca ccagcggctc gtcctcgacc
    11041 acgagcacgc gccccttgcg ggcagcagcc accgggcact ccggccgcgg ggctggcggc
    11101 ggcgacaggg ccgacgacag ccgggcatcg gccaggttgt cgaacgtctc ggccagcggc
    11161 agacgaatgg cgcaggcgag gccgtcgccg gcgtagctga ccctggcctc gccccggttg
    11221 gcctcgaggc tgcgctcgat cagcgtcgag ccgaagccct tcctccccgg cacctccagc
    11281 cccggcacgc cgctctcccg ccaggtgaga tgcagctcgc ggcctgccgc cacggcgacc
    11341 tcccaggtga tcgacagccg gcctccgggc acggccagcg caccatgctt gcgcgcgttg
    11401 gtggcgagct cgtgcagcac cagcgcgagc tgcaccgcca cccgggcatc gagcaccacc
    11461 cggggccccg aggtgacgat gcgggcgcca tcgccactgc cgagcagcac ctgctcgcgg
    11521 acgagatcgc cgagcgtggc gccccgcatc ccgccctgca ccagaaggtc gtgggcccgg
    11581 gcgagcgcct ggacccgccc gttgaaggcg gcgacgaaag cttcggggct ggtggtgctg
    11641 cgcatcgatt ggctggcgat cgcctggatg gtcgccagcg tgttcttgac ccgatggttg
    11701 agctcgctga agagcagggc gcgcatctcc tcgctgcgct tgcgctcggt gacgtcgcgg
    11761 gcgaccttcg aggcaccgac gatgctgccg ctgctgtcgc ggatcgggga gatggtgagc
    11821 gagatgtgga cctggcggcc gtccttcgcc acccgcaccg tatcgaaatg gtcgatccgc
    11881 tcgccggccc ggagccgcgc caggatctcg tcctcctcct gctgcagatc cgccgggatg
    11941 atccgcttga tcgaccggcc gatcatctcc tcctgggtgt agccgaagat ccgcacggcg
    12001 cccgcgttcc atgacgtgac gatgccgtcg agggtcttgc tgacgatggc atcgtcggag
    12061 gaggcgacga tcgccgccag gtgcagcgcc gcgatctcgg actgccgggt gtcgccactg
    12121 tcggccagca gcatgaccgc ccccgtcagc ttgcccgagc cgtcgcgcag cgggccggca
    12181 tgggccgcga gcgcctgccg ggcgccatcc tcgcgaacga ggatcaccgc cctgcccgac
    12241 caggcctgcc catcgcggag caccaccgcg agcggcgtct cctccgggcg catggcccgg
    12301 ccgtccggcc agagaagatg ccaggagccg tcgtctatcc ggctgccgag ggccggccgc
    12361 tcgccccaga gggtgaccgc cgcttcgttc ctgaacgtga cgcgcccctg cgcatcggtg
    12421 gcatacaccg ccaccggcag ggcctcgagc agatccgtgc ccagggtcat gtgctcgcca
    12481 tccgtcccat cgccttcaac cgctcgaacg acaccgggtt cccccattct ctacagcaaa
    12541 gggcagccgg tcagcagggg cgaatgcaga agggggccgt cccgccctgc ttgacacatg
    12601 ccatggcacc attaagttag taatcactca cttagcaact gaacgcccat gctgatccgc
    12661 aagcccgccg ccacccgcaa ggccgagatc gtcgaggcgg cccttcgcct cgccgacgag
    12721 ctcggccccg accgtctgac gacggaggcc atagccggtg cggtgggtct gacgcagccc
    12781 gccatttttc gccattttcc caccaagcag gatctgtggg aagcggtcgg ggcacgcatc
    12841 ggcgagctga tggaagaacg ctgggctgcc gttccggccg acgaaggccg ggcgaccgac
    12901 cgggtccgcg ccctcgtcgc gacgcaactg gggctgatcc aggccacgcc ggccatcccg
    12961 gccatcctct tctcgcgcga gctgcacgtg gagaaccagg gcctgcgccg agccttcctc
    13021 gagctgatga gccgcttcca ccggctgatc gccgggctcc tggcccaggc acgcgacgac
    13081 ggcgagctct gccccgagct cgaccccgac gatctggccg ccctcgtcat cggcctgccg
    13141 ccgggtctgg cggtcagatg gtcgctcagc gaccggggat tcgacctggg cgaggaaggc
    13201 aggcggctgc tggagctgca gcttcgcggc ttcaccagcg aagcgacgag gagatcgtct
    13261 tgagcagccg cctcgtccgc ctgcttctgg ccctcctggc tctcggcgcc accgcattgg
    13321 cgatcttcct cttcgtcttc atgcgcccgt tgccggtgga ggtcgctcga cccgcgagcc
    13381 aggtgccgat cgaggtcttc gggctcggca cggtcgaggc gcgcattctc tcggagatcg
    13441 gcttcgaggt cggcgccgcc ctcgtcgagc tgaatgccga tcacggcgac cgggtgcggc
    13501 agggcgacat cctggcacgg ctgcatggcg ccgagcagga ggcccgggtc gccagggccc
    13561 gggcgggtgt ggtcaatgcc gaggcggcgg tgcaaaggat ggacgccgcg gtcggccggg
    13621 ccgaggcggt gctggcccag cgggcgcaga ccaaccggcg tcgccgggag ctcgtcgccc
    13681 gccaggcgat ctcgatcgag accgccgagg aagcggagat ggaggaagcg gtcgccaggg
    13741 ccgagctggc cgtggcgtcg agcgacgtcg atgtggccga ggcggcgctc gatgacgccc
    13801 gggcgcagct tcagatggag caggtgctgc tcgagcatca cgtcctgaaa gcgccctatg
    13861 acgcgatcgt cgtggcgcgc catcgcgagc tcggctacgt cctcgacccc ggcgagccgc
    13921 tcttcaccct ggtcgccccg gagaccgtct ggacgctggc ctatgtcgac gaggcgcggg
    13981 ccggcggtct ccgcgtcggc cagccggccc tcgtgcatct ccgctcccgg ccccatgaga
    14041 tgctgcaggg ccatgtcacg cggatcgaca tcgagagcga tcgggtgagc gaggagcgcc
    14101 gggtctatgt cgcctgcgac cagtgccccg agagcttcca tctcggcgag caggccgagg
    14161 tcttcatcac cacgaccgtg ctcgagtcgg cgctcctcgt gcccgagatc gcggtcgagc
    14221 acttcgacgg cacgcgcggc acggtctgga ccgtcgagga cggcgtgctg caccggcgcg
    14281 cggtcacctt cggcgagcgg acgctggatt cccgcctcgc cgtcagcgac ggcctgcccg
    14341 ccggcgccga ggtcgtcagc accttgcgcc cgggcctgcg cgaaggccgc cgggccaggg
    14401 tgacggaggc cggggcacca tgaacctcgc ctaccgcgac gtccgccaca atctcggtcg
    14461 cttcctcctg acctgcttcg ggctcagcct gctgctcggc gtcgtccttt cgatgatcgg
    14521 catctaccgg ggcctggtcg aggatgcgct gacgctggtc cgcacgccag ccgtcgacgt
    14581 ctgggtggtg gaatccggca cgcgcgggcc gttcgccgag gcctcgcgca ttcctggcga
    14641 cacgcgggaa gccatcgccc gcctcaccgg ggtaaccgag gcgggcagcg tgacctacca
    14701 gtccgtcgag acgctgctca tgggcaccaa gctccggctc tacgtcgtcg gctacgagcc
    14761 gggccggccg ggtggccccg accggatcac cgccggccgc cccatcggcc gcagccacta
    14821 cgagatggtg gccgaccggc gatcgggcct cgccctcggc gaccgcctgc ccctcggccg
    14881 caacaccttc accgtggtcg gcctgaccga cggacaggtc tcgtccggcg gcgatcccgt
    14941 cgtctacctg acgctcctgg attcccagaa gctgcagttc gagctggccc cgcccgcggc
    15001 gcggcgggag ctcgcccggg gcggcggcca gggtggcacg gacacgatca acgccgtcgt
    15061 cgcccggctc tcgccgaatg cgtcgcccga gaacgcggcg aatgccacca gacgctggaa
    15121 gcatctgtcg gcgatgaccc aggacgggca ggagacgatc ctcacccgct cggtggtcga
    15181 gcgggcgcgc aagcagatcg ggctcttcac caccctcctc ttgatcgtct ccgccgtgat
    15241 catcgcgctc atcctctaca ccatgaccat ggacaagatc cgcgagatcg ccacgctcaa
    15301 gctcatcggc gctgcggacc gcacgatcat cggcctgatc ctgcagcagg cgctggccat
    15361 gggcgccatc ggcttcggct tcggcgccct gctgatcacg acggtcaagg actatttccc
    15421 gcgtcgcgtc gtgctgcagc cggaggacgg cctggcgctc ggggccgccg tgctcgtcct
    15481 ctgcgtgctc tcgagcgccc tcggcgtgcg gctggcgctc cgggtcgatc ccgcgaatgc
    15541 cctgggcggc tgacgatgca ggcagccgcc ggcaaccgcc cgctcgtccg ggtggcgcat
    15601 gtctccaagc atttcggcgc cggcgagacg cgcgtcgacg cgctccggga cgtcagcctc
    15661 gaggtccaac cgggccaggt ggtggcgctg ctcggcccga gcggctcggg caagaccacg
    15721 ctgctcaacg tgatcggctg catcaccgag ccgagcagcg gccgcctcga gctggacggc
    15781 gaggccgtct atgacggacg ctggctcagg ggcgatctgc gcaagctgcg gctggagaag
    15841 atcggcttca tcttccagtt ccacaatctg ctgcctttcc tcagcagcac cgacaacgtg
    15901 gcggtggtgc tcagcctcgc cgggctgggc accgccgcgg cgcggcggcg ggccgtcgag
    15961 cttctggact atctcgaggt cggccaccgc cgggacgcca tgcccgccaa gctctccggc
    16021 ggcgaggcgc agcgcgtcgc catcgcccgc gccctcgcca accggccgcg catcatcctc
    16081 gccgacgagc cgaccgcggc gctcgattcc gagcgggcca ggatcgtcat ggacctcctg
    16141 cgcaagctcg ccaccgagca gcaggcggcg atcctggcgg tgacccacga cgagaagatc
    16201 ttcgaccgct tcgaccacat ctttcacctg cgcgacgggc gcctggacaa tcaggagcca
    16261 tgagcggggc ccggtcgcgg catggcgacc gacagccgaa gacccgaccg accgtcgctc
    16321 agtttttatt tataccattt ccaaacaact tgctttcgga agtactccga agttttgtac
    16381 ttgacctgtc ttatgattgt ctgctctgga cgtcaatggc cgggatcgtc aggcaaaggc
    16441 cgccggcaca aggccgctcg atgggccgcg ggccggacaa catcacgcaa ggagacactg
    16501 cccatgcgcc cctgtgtatt catccacacc aaccacaagc aatatgtcgg ggcgctggtc
    16561 tcggcctact cgttccggcg cttcgccaag gatcccgacg cgttcgacgt gcagatcatc
    16621 cacaccaagg atttcccgtt cctgcgcgag cacgagggcg aggcgtatct gcgtgacggc
    16681 gtcaagcgcc actggcgcta cgacgacctg cagtcgttca ccaccctgcg cttcatgccg
    16741 cccgagcgca tgggctacga gggccgctcg gtcgtggtcg atccggacgt cttcgccgtc
    16801 ggcgatgtcg gcgagctcct cgcccgcgac atgggtggca aggcgatcat cgcccgaaca
    16861 cgcccgggcc cgcgcacgct caccgatggc ggccgctaca acagcagcgt gatgctgctc
    16921 gacaacgcca agctgcggca ctggcgggtc gaggagcagt tcgaggcgat gttcgagatg
    16981 cagcgcgact atcgccgctg gatcacgctc ggctacgagg acagcgccac gatcggcgag
    17041 ctggagcgcg agtggaacga cctcgaccac ctcgatacca ggacgaggat gatccacaac
    17101 acccatcgca agacccagcc ctggaagagc ggcctgccgg tcgacttcat cccggccgag
    17161 cggttccgcc cgttcccgcc gatcgcctgg gcgctccgtc tgcggcgcaa gctgttcggt
    17221 gactacgggc tgctcggcac ctacaagccg catcccgaca ggaaccagga gaagctcttc
    17281 ttcggcctcc tgaaggagtg cctggccaac ggcctcgtca ccgaggagct ggtgcgcgag
    17341 gagatgcgcc agaaccatgt ccgcaaggac gcgctcgacg tgatcgcggc ggccccgccc
    17401 ctgtcgtcgc tgcccctgtc ggcgctgccg cgcgccgcct gagccggcgg caccggctgg
    17461 tcgcccgcgt cacacggcgc aggcggtcac cggtgccgcg tcgcccttcg gcgtcagtcg
    17521 tagttgccga gcaggatgcc gatcagctcg cggccggcgc cgcccgccag gttgccattg
    17581 ccgagcagga tgccgagatt ctcccggccg ctgccgccac cctcgttgcc gttgccgacg
    17641 aggacaccga tgttctcccg gccgctgccg ttgccggcgt tgccgttgcc gaccacgatg
    17701 ccgacattct cccggccgct ggcgtcgccg gcattgccgt tggcgaccag cacgccgatg
    17761 ctctcgcggc cgctgaagtc gccggaattg tcgttgccca cgaggatgcc gacattgtcc
    17821 cggccgctgt tctcgccggc attgccgttg ccgctcatga tcccgacatt gcccgtgccg
    17881 gcgccgtcac cgg
//
LOCUS       DAOTFI010000093        17886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3787_length_17886_cov_6.839942, whole genome shotgun sequence.
ACCESSION   DAOTFI010000093 DAOTFI010000000
VERSION     DAOTFI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17886
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3787_length_17886_cov_6.839942"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="P5558_11130"
     CDS             <1..625
                     /locus_tag="P5558_11130"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY24907.1"
                     /translation="MAVYTLSFWHYLVYALAFLWRRITPERFQRDAILLRTISLVALA
                     GVCLAILPNLFSLVVMAAGFSLNVAAYRALGTDRTYYGVELTALPPERTTAFPYSLTA
                     HPMLIGNMLGYGAPLLDAGFRAEWWPLAALHALLNLAIILMEAHGRQSRRLGTLVPLL
                     GLAAGSLLLLIAFSDVWPFALASVLCIVAFGALLFRRYATPAGPGDT"
     gene            complement(680..1510)
                     /locus_tag="P5558_11135"
     CDS             complement(680..1510)
                     /locus_tag="P5558_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24908.1"
                     /translation="MATATAETSRKTGSWGRSGPPLIEVDNITKHFGSVIALGGVSLS
                     VDRHEVHCLLGDNGAGKSTLIKTLSGVHKPSSGTFRVEGQPVAFDSPREALDAGIATV
                     YQDLAMIPLMSITRNFFMGREPLKGIWPFRFFDFGEADQVAREEMARIGIDVRDPEQA
                     VGTLSGGERQCVAIARAVYFGAKVLILDEPTSALGVRQTSMVLKYINMVRQKGLGVIF
                     ITHNVRHAHAVGDRFTVLNRGQTLGTYGKDEVNLEELQNLMAGGKELQDLSAELGGTV
                     "
     gene            complement(1528..2619)
                     /locus_tag="P5558_11140"
     CDS             complement(1528..2619)
                     /locus_tag="P5558_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24909.1"
                     /translation="MAMADERLKQASFMTRLMRRPELGAFSGLVLVSIFFYILASGQM
                     FTLSGILTFMSPAAQLGILAIGAALLMVGGEFDLSVGSMVAFAGLVFGASVTALHLPL
                     LPAILLTLAMAAAFGTLNGQIVLRTGLPSFIVTLAFLFILRGLTLVGLKWATGGSTQM
                     RGIREAVGDSVLAPLFSGQALEGLFAWLAERGMIETFSSGLPKVTGIPVDILWFFGIA
                     AVATIVLTRTRVGNWIFAAGGDANAARNSGVPVARVKTVLFIVTACCAALVAILTVMD
                     TGSTDARRGFQKEFEAIIAAVIGGCLLTGGYGSAIGAFFGAIIFGMVLIGLTYTQIDQ
                     DWYLVFLGGMLLIAVFFNNIVRKRATGER"
     gene            complement(2720..3655)
                     /locus_tag="P5558_11145"
     CDS             complement(2720..3655)
                     /locus_tag="P5558_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY24910.1"
                     /translation="MTLTKTLTAAALAAVLTAGTALAQDTSIIVVSHGQASDPFWSVV
                     KNGVDEAARDYGVNVDYRAPETFDMVAMGQLIDAAVNQEPYGLVVSIPDADALGPSIQ
                     RAVDAGINVISMNSGSDVAHGLGALLHVGQSEFDAGKIAGAELASMGGSNGICINQEV
                     GNVALDLRCAGFAEGFGGNVTILPVTMDPAEITAKVKAAMETDASIDTILALGAGTAG
                     EPALRALEETGKAGEINLASFDLSANFLQAIVDGKAAFAIDQQQFLQGYLPIAFLALQ
                     SRYGLMPGGDVPSGPNLITADKAGQVIELSAKGIR"
     gene            3817..4662
                     /locus_tag="P5558_11150"
     CDS             3817..4662
                     /locus_tag="P5558_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRY24911.1"
                     /translation="MKQPPTLMPESFSDLEQLIAARHGGLSTRLKDIAAFVLAHPNTI
                     ALETVATVAEQARVPPSALVRFAQALGFDGFSDMQRLFRLRLVEQMPSYADRLAQRES
                     GDAAPGDSLDRLAQAAHRAIDELAVETPRADLAAAADRLAGARRILIVAQRRSLPVAT
                     YLLYGLLNLERPVQLLDNQGGMLGQQLKTLARGDVLLAASYRPYAPETLEAVRAAEAQ
                     GLGIVVITDLQLSPLAAHGRPTLFVRDAEIQNVRSLSASMCLATSLVIETGKRLVGGR
                     GRRQE"
     gene            4766..6685
                     /gene="iolC"
                     /locus_tag="P5558_11155"
     CDS             4766..6685
                     /gene="iolC"
                     /locus_tag="P5558_11155"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HRY24912.1"
                     /translation="MLPPALDVVTIGRSSVDLYGEQAGGRLEDMSSFAKYVGGCPTNI
                     AIGTAKLGLHSGLITAVGDEHMGRFVKEEVERWGVDTGRLKTDPARLTSLVILGIRDE
                     HSFPLIFFRENCADSALDEGDIDEAYIAGAKAVVVTGTHFSRPNLDRASRKAATVARA
                     KGRKVILDIDYRPVLWGLTGHGLGEERFVESGNVSAHLQTILPLCDVVVGTEEELHIA
                     GGSTDTLAAIRTVREHAPDALIVCKRGPMGCVAFPGAIPDDIEEGIKGPGFPVDVYNV
                     LGAGDAFMSGFLRGYLRDLPVLECCRLANACGAFAVSRHGCAPAIPSWIELSHFLEHG
                     SPERALRKDRTLEQLHWSTNRERHFPQVLAFAFDHRSQLEAIADEAGVSRPHIAHFKS
                     LCWLAVERQRQGRSDLGILLDGRYGGATLPRATGQGIWIGRPIEQPGSRPLRFDDGPD
                     LGRVLREWPREHCVKCLAFYHPDDPAELRELQVERLQILQDAARGTFHDLLIEIIASK
                     SGLPEETTTLARAIRQLYGAGLFPDWWKLPDPGSDAAWRAVADTVGAYDPHCRGILLL
                     GLEAPEATLEEAFRRAADQPICKGFAVGRSIFGSAALDWFSGKIDDAAAIDRMAATYG
                     RLIEAWDRARAGGST"
     gene            6682..7479
                     /gene="iolB"
                     /locus_tag="P5558_11160"
     CDS             6682..7479
                     /gene="iolB"
                     /locus_tag="P5558_11160"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRY24913.1"
                     /translation="MSRLLLKNHVPDAAGLVHEVTPESAGWGHVGFALHRLAKGQRLE
                     VKGSADETCIVLVTGSATVEAGPQIFRSIGGRASPFEGKPTAVYVPRDTAFAVIAESE
                     LELALCTAPGGGEHGVRLIGPDDVAYSRRGQGTNTRHVHDILPETEPAHSLLVVEVVT
                     PAGGWSSYPSHKHDTDNLPHESLLEEIYYHRFQPPQGFGFQRVYTDDRSLDETMTIED
                     GDLVMVPKGYHPCGTAHGYDLYYLNVMAGPKRAWRINTEECHRWLLT"
     gene            complement(7483..8928)
                     /locus_tag="P5558_11165"
     CDS             complement(7483..8928)
                     /locus_tag="P5558_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRY24914.1"
                     /translation="MIELGPVARVIGLLLGVLALVMLVPALVDVTAGNPDWLSFVVSA
                     AIAGFFGGMLILVTSRPGAFQLDLRQAFLLTALTWVVVPAFAALPMIGLGHGYTDAFF
                     EAMSGLTTTGSTVLAGLDGLPPGILLWRSLLQWLGGIGIIVMAIVLLPFLRIGGMQLF
                     RTESSEQSEKVVPRAFHMVASIGLVYLGLTIACAVAYALAEMSVFDAVNHAMATLSTG
                     GYSTHDLSFGYFQRPLTHWIGTIFMIAGALPFLAYIRVLRGDRGALLRDTQVKALFVF
                     FFLVIGSKAAWLAVTREMAFDEALQHTAFNVVSVVTTTGFASTDYTLWGPGAEAVFFV
                     LMFLGGCAGSTSGAIKVYRHLIIWQLIRLKLRRLVAQHQVVPLRYGGSRVPEDVPGAV
                     LAFVAALLGMVAIFTVLLGLMGLDLVTALTASATAITNVGPGLGPIIGPAGNFASLPD
                     AAKWLLALAMMLGRLEIFTVLLLFTPDFWRR"
     gene            complement(8948..9349)
                     /locus_tag="P5558_11170"
     CDS             complement(8948..9349)
                     /locus_tag="P5558_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY24915.1"
                     /translation="MPQKILLPIDLTSPEAWRHAIDAAEDLARRYGATLAVLWVAPPA
                     AADPATLPETYLPALHDFVAGRFADDITVEAHVRADWGDPVREICHAAAEVGADLIVM
                     GTHMPQADESLDHSNAAQVALQAGCSVWVVR"
     gene            complement(9425..9838)
                     /locus_tag="P5558_11175"
     CDS             complement(9425..9838)
                     /locus_tag="P5558_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY24916.1"
                     /translation="MYKVIAVPVDLAHVDRLTKALETAADLAKAYGATLYYVGATTET
                     PSSVAHTPKEFKGKLEQFAAERGTALGIDARARSLVSLDPAVDLDRDLLKTFKEIDAD
                     LVVMASHIPGIKEHLISSNAGWIASHAKISVFVVR"
     gene            complement(9860..11434)
                     /locus_tag="P5558_11180"
     CDS             complement(9860..11434)
                     /locus_tag="P5558_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HRY24917.1"
                     /translation="MSEPVQPDFTVDSTDYEVGQDNVQILGLDIHNPVFMISAVTIVG
                     FVIFTLMFQEGTAAFFGWLRPWLTSTFDWVFMSAANIFVLFCLFLVISPWGRIRLGGA
                     DATPDFSYAGWFAMLFAAGMGIGLMFFGVLEPVYHFQNPPLGVNPEDVEAARAMGMAA
                     TIFHWGLHPWAIYAVVALSLAFFTFNNGLPLTIRSAFYPLLGDRVWGWPGHIIDTLAV
                     FATLFGLATSLGFGAEQASAGLEYLFGIPATDVTKVALIIAITAVALISVVAGLEAGV
                     KRLSEINMVLALALLLFVIIAGPTIALLTGFFANAGHYAADLLPLSNPFGRDDTAFMH
                     GWTTFYWAWWISWSPFVGMFIARVSRGRTVREFITCVLIIPTLVSILWMTAFGGTAID
                     QIVNDNYQGVAETVAAYVPELSLFKMLEVLPLTMVSSFLGIVLVIVFFVTSSDSGSLV
                     IDTITAGGKTDAPVAQRVFWATFEGLVAIALLLGGGLASLQGAAIATGFPFAVVLVAM
                     CWCLFKGLRADPQMAR"
     gene            complement(11613..12815)
                     /locus_tag="P5558_11185"
     CDS             complement(11613..12815)
                     /locus_tag="P5558_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY24918.1"
                     /translation="MSEPLFTLSAIATDTAVLLMLGAVLALSLMVDVLARRLGLPRVS
                     LLVLTGVAIATAARVLLEQEVTVLTAAVAEPLVSIALVMVAFLLGGDLTAERWRLTGR
                     PVLALSLAIALVSALLVGGGLLALGFLPAIALPLAAMAVATDPAAVHEVVREAGGGGR
                     VGQVVLGIVAIDDAWGIIAFGVAVSLLELVAGADGWHAVGRAGWELGGALLLGLAIGA
                     PAAFLTGRLRPGEPTQVEAVAVILLIAGLADALAVSPLLAGMTAGVAVANLTPHHSRS
                     FSEIEHIEWPFLVFFFVAAGAAADPARLGAAAPLTLAYLALRVAGRLLGGWAALPWLA
                     GPDRRDFATVGLGLLPQAGVAMGMALLAAERYPAAAADLLAVAVAATIFFEIAGPLLT
                     RRQLGRAA"
     gene            12935..14065
                     /locus_tag="P5558_11190"
     CDS             12935..14065
                     /locus_tag="P5558_11190"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HRY24919.1"
                     /translation="MPEIFNHHFTRSELTRHVGDTSQLFGVDLVTHEDGPERGLRVLR
                     FRTGGGLELEVLVDRAMDVGAMSVCGVPLGWRSGNGFRHPWLHEHDAEGGLGWLRSFS
                     GLVNTCGLDHIMGMAEEGADHFNYPYRARVAHGIHGRVAFTPARLQGFGERWDGDDCV
                     LHAEGTVRQVTMFGENLELARRIEATVGGRTVKITDRVTNLGYRTTPHALLYHVNAGW
                     PVVAAESRLSLPLERTVYHAHDPHATSIGPLEQAAPQVGFREQVYEHALRRRADGMVP
                     VLLANPDFGGLSGSRGIGLAMRYDGRSLPAFYQWQNLHEGDYVIGIEPATCHAGSRED
                     WKKRNEMIWLEHGDSREYRLELEGVVGEAALEAMVDEIDGLR"
     gene            14052..15656
                     /locus_tag="P5558_11195"
     CDS             14052..15656
                     /locus_tag="P5558_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRY24920.1"
                     /translation="MASARAVLDQPGTHWRIETAARAALLIDGDAYFQALAAAMEKAE
                     RSIIILGWDLRSDLLLDPMNSRETLAARLQRLIGARAGLHVRILVWDWPIVMGAGREV
                     LPHWSFGPLEERLTFVLDDEIPVGGAHHEKLVVIDDRLAFVGGLDLTAGRWDTPEHHP
                     GNLRRLEIESGLDRAPFHDVMLMVEGPVAAALGELAAERWRVATDDHLAPSGSSGTDL
                     WPEGIEADAQDVPVAIARTRPEIDGLEAAREIEALYLAAIAGAERLIYIENQYLTVPA
                     VARALARRLRDMPDLEVVIITPKACEGPLETAVMDAGRKKFKATLEAAARDRVRVLTA
                     QSHGLRVNVHAKLMAVDDRLFTVGSANLANRSMGVDTETNLAIERPEPDPVIRGWRHR
                     LLAEHLGREPGEVAACEAELGSTIAMIERLSDHEAAHYLAPLDLDAHPIPEPLSDIVD
                     VAELADPPEPIIGSQLLAELGLPRRFRNLRRWLGRVAGLVGVAVLLAWFLGAIDLVSG
                     WVVAGASLALVLGWAAGERPWRLERH"
     gene            15656..16435
                     /locus_tag="P5558_11200"
     CDS             15656..16435
                     /locus_tag="P5558_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY24921.1"
                     /translation="MRVATYNIHGAVGLDRRRRPERIVEVIESLDADIIGLQEVEGRR
                     SRSRVDQAQHIGDALDYHLVAGPLLLEGMGGYGNVLLSRHPVLEVERRVYQRPGSQTR
                     GLLDAVVAHPLLGRLRVIVTHLEVRDHRIRATQLRELESLLAEGPEGPAVLMGDLNEW
                     WSRRVALKSLDRTVRFLHSPATFPARLPILRLDRIGVRSLRSTDPDGRARRVETRLTR
                     IASDHLPLVADLEPLEALPAPEDEDQASSTSSRHSTPLARQ"
     gene            complement(16384..17160)
                     /locus_tag="P5558_11205"
     CDS             complement(16384..17160)
                     /locus_tag="P5558_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRY24922.1"
                     /translation="MATDGEQKKGKVTAGLLIIGNEILSGRTVDANLPYLATFLGGIG
                     IRLVEARVIPDIEREIVDAVNAMRVKVDYLFTTGGIGPTHDDITAGCIARAFGQKLIR
                     HPEAERRLRAFYPPEKVTEARLKMAETPEGAVLIDNPVSVAPGFIVENVHVLAGVPKI
                     MQAMLDILKPRLVGGALVHSRTLVVFKGEGDMAAVLGAIQGRYPAIDIGSYPFFRMDK
                     PGTSIVFRGDDVPAIDAAFAELEAYCRASGVECREEVLLA"
     gene            17202..>17886
                     /gene="sfsA"
                     /locus_tag="P5558_11210"
     CDS             17202..>17886
                     /gene="sfsA"
                     /locus_tag="P5558_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HRY24923.1"
                     /translation="MDLPVPLHRATLRHRYKRFLADVTMADGRELTAHIADPGRLPGL
                     AVPGADLWLSRSDSPKRKLAHRVELVETPESLVGLDTGNANRLVAEALAGGRLPGLED
                     WQATAREPRLHAGTRLDFALRASGGRSAFLEVKNATWRRGAVAAFPDAVTARGTRHLQ
                     ALAGLAAAGTPAFLLFVCQRSDVAALTIAADIDPAYAAAFELARAAGVAILACRCKLN
                     HCAITITEGI"
BASE COUNT         2866 a         6182 c         6267 g         2571 t
ORIGIN      
        1 catggcggtc tacaccctca gcttctggca ctatctcgtc tacgcgctgg ccttcctctg
       61 gcggcgcatc acgcccgagc gcttccagcg cgacgccatc ctgctgagga ccatctcgct
      121 cgtcgccctc gccggcgtct gcctcgccat cctgcccaac ctcttttcgc tcgtcgtcat
      181 ggccgccggc ttctcgctca atgtcgcggc ctaccgcgcg ctcggcacag acaggaccta
      241 ttacggcgtc gagctcacgg ccctgccgcc cgagcggacg accgcgttcc cgtactcgct
      301 caccgcgcat ccgatgctga tcggcaacat gctgggctat ggcgcacccc ttctggacgc
      361 cggtttcagg gcggaatggt ggccgctggc agcactccat gccctcctca acctcgccat
      421 catcctcatg gaggcgcacg gccggcagag ccgtcggctc ggcacgctcg tgccgctcct
      481 cggcctcgcg gccggatcgc tcctgctcct gatcgccttt agcgacgtgt ggccgttcgc
      541 cctggcttcc gtcctctgca tcgtggcctt cggcgccttg ctgtttcgcc gctacgcgac
      601 gccggccggg ccgggcgaca cctgagaagc cccatcgcgg ctccggccac gtcttcgcac
      661 acccgggcag gacgcttttc tagaccgtcc cgccgagctc ggcggagagg tcctgcagct
      721 ccttgccgcc ggccatgagg ttctgcagct cctcgagatt gacctcgtcc ttgccgtagg
      781 tgccgagcgt ctggccgcgg ttgaggacgg tgaagcggtc gccgacggcg tgggcgtggc
      841 ggacgttgtg ggtgatgaag atgacgccga ggcccttctg gcggaccatg ttgatgtatt
      901 tgagcaccat cgaggtctgc cgcacgccga gcgccgaggt gggctcgtcg aggatcagca
      961 ccttggcgcc gaaatagacg gcgcgggcga tcgccacgca ttgccgctcg ccgccggaga
     1021 gggtgccgac cgcctgctcg gggtcgcgga cgtcgatgcc gatccgcgcc atctcctcgc
     1081 gcgccacctg gtcggcctcg ccgaagtcga agaagcggaa cggccagatg cccttgagcg
     1141 gctcgcggcc catgaagaag ttgcgggtga tcgacatgag cgggatcatc gcgaggtcct
     1201 ggtagaccgt ggcgatgccg gcatcgagcg cctcgcgcgg gctgtcgaac gcgacgggct
     1261 ggccctcgac ccggaaggtt cccgagctcg gcttgtggac gccggagagc gtcttgatca
     1321 gggtcgactt gccggcgcca ttgtcgccga ggaggcagtg cacctcgtgg cggtcgacgc
     1381 tgaggctgac gccgcccagg gcgatcaccg agccgaaatg cttggtgatg ttgtcgacct
     1441 cgatgagggg cgggccggat cgaccccagg agccggtctt gcgggacgtc tcggccgttg
     1501 cggttgccat caggggtccc ctccgtccta ccgttcgccg gtcgcccgct tgcggacgat
     1561 gttgttgaag aagacggcga tcagcagcat gccgccgagg aagacgaggt accagtcctg
     1621 gtcgatctgg gtgtaggtca ggccgatcag caccatgccg aagatgatgg cgccgaagaa
     1681 ggcgccgatc gccgagccgt agccgccggt gagcaggcag ccgccgatga cggcggcgat
     1741 gatcgcctcg aactccttct ggaagccgcg cctggcatcg gtgctgccgg tgtccatgac
     1801 ggtcaggatg gcgacgagcg ccgcgcagca ggcggtgacg atgaagagca ccgtcttcac
     1861 ccgggcgacc ggcacgcccg agttgcgggc ggcgttggcg tcgccgccgg cggcgaagat
     1921 ccagttgccg acccgggtcc gggtgaggac gatggtggcg acggcggcga tgccgaagaa
     1981 ccagaggatg tcgacgggga tgccggtgac cttgggcagg ccgctcgaga aggtctcgat
     2041 catgccccgc tcggcgagcc aggcgaagag gccctcgagg gcctggccgg agaagagcgg
     2101 ggccagcacg ctgtcgccga ccgcttcgcg gatgccgcgc atctgggtcg acccgccggt
     2161 cgcccatttc agcccgacca gggtcaggcc gcgcaggatg aagaggaagg cgagcgtgac
     2221 gatgaaggag gggaggcccg tgcgcagcac gatctgcccg ttgagcgtgc cgaaggccgc
     2281 ggccatggcg agcgtgagca ggatggcggg caggagcggc agatgcagcg ccgtcacgga
     2341 agccccgaag acgagcccgg cgaaggcgac catcgagccg accgagaggt cgaactcgcc
     2401 gccgaccatc agaagtgcgg caccgatcgc gaggatgccg agctgggctg ccggcgacat
     2461 gaaggtcagg atgccggaga gcgtgaacat ctggccgctg gccaggatgt agaagaagat
     2521 cgagaccagc acgagcccgg agaaggcgcc gagctccggg cgccgcatca gccgcgtcat
     2581 gaaggacgcc tgcttcagcc gttcgtcggc catcgccatc gccgcctccc caggcttctg
     2641 gttgggttct gtcgtgccgc gtgtcagccg gccatcggga ccgaccctga cggtcggtcc
     2701 cgatggttcg gacagtggct cagcggatgc ccttggcgct gagctcgatg acctgcccgg
     2761 ccttgtcggc ggtgatcagg ttcgggcccg acggcacgtc gccacccggc atcaggccgt
     2821 agcgcgactg cagggcgagg aaggcgatcg gcagatagcc ctggaggaac tgctgctggt
     2881 cgatggcgaa ggcggccttg ccgtcgacga tggcctgcag gaagttggcc gagagatcga
     2941 agctggcgag gttgatctcg cctgccttgc cggtctcctc cagcgcccgc agcgccggct
     3001 cgccggcggt tccggcgccc agcgccagga tcgtgtcgat cgacgcgtcg gtctccatcg
     3061 ccgccttgac cttggcggtg atctcggccg gatccatggt caccggcagg atcgtgacat
     3121 tgccgccgaa gccctcggcg aagccggcgc agcgcaggtc gagggcgaca ttgccgacct
     3181 cctggttgat gcagatgcca ttcgagccgc ccatcgaggc gagctcggcg ccggcgatct
     3241 tgcctgcgtc gaactccgac tggccgacat ggagcagggc gccgaggcca tgcgccacgt
     3301 ccgagcccga attcatcgag atgacgttga tgccggcatc gacggcgcgc tggatcgacg
     3361 ggccgagcgc gtcggcatcg ggaatcgaga cgacgaggcc atagggctcc tggttgaccg
     3421 cggcgtcgat gagctggccc atcgccacca tgtcgaaggt ctcgggtgcg cggtagtcga
     3481 cgttgacccc gtagtcccgg gcggcctcgt cgacgccgtt cttgaccacc gaccagaacg
     3541 ggtcggaggc ctggccgtgg ctgaccacga tgatgctggt gtcctgcgcc agggcggtgc
     3601 cggcggtcag cacggcggca agagcggcag ccgtgagcgt tttcgtcaag gtcatggaaa
     3661 cctcccggtt tgctgatgca gatcgccttg tccgacagcg ctgtcagaac agcgtttcca
     3721 tctgcttggg tttatggaat acacattcca gcggaaggtt caagcccctg gaacaagaat
     3781 tccaaattcg caccatctgg aacgcgtgcg ccaccaatga agcagccgcc caccctgatg
     3841 cccgagagct tcagcgatct ggagcagctc attgccgccc ggcatggcgg cctgagcacg
     3901 cggctcaagg atatagcggc cttcgtgctc gcccatccca acaccatagc gctggagacc
     3961 gtcgccaccg tcgccgagca ggccagggtg ccgccttcgg cgctggtgcg cttcgcccag
     4021 gcgctcggct tcgacgggtt cagcgacatg cagcggctct tccggctcag gctggtcgag
     4081 cagatgccga gctacgccga ccggctggcg cagcgcgaga gcggcgatgc ggcgccgggc
     4141 gacagcctcg accggctggc ccaggcagcc caccgggcga tcgacgagct cgcggtcgag
     4201 acgccccgcg ccgacctcgc cgccgcggcc gaccggctcg ccggggcgag gcgcatcctc
     4261 atcgtcgccc agcggcggag cctgccggtc gccacctatc tgctctacgg cctgctcaat
     4321 ctcgagcgcc cggtccagct cctcgacaac cagggcggga tgctcggcca gcagctcaag
     4381 acgctggccc ggggcgatgt gctgctggcc gcgagctatc gcccctatgc gccagagacg
     4441 ctcgaggcgg tgcgtgccgc cgaggcgcag ggtctcggca tcgtcgtgat caccgacctg
     4501 cagctcagcc cgctcgccgc gcacggccgg ccgacgctct tcgtccgtga cgccgagatc
     4561 cagaatgtcc gctcgctgag cgcttcgatg tgcctggcga cgagcctcgt gatcgagacc
     4621 ggcaagcgac tggtgggcgg ccggggccgc cgacaggaat aggcattcca ggcttgaaca
     4681 gcgctggaat gcatgtttca ttgggtggta tcagggaacc gccgaaagcc gacccatgcc
     4741 gaatgatgcg cccgtatccg accccgtgct cccccctgcg ctcgacgtcg tgaccatcgg
     4801 ccgctcgtcg gtcgatctct atggcgagca ggccggcggg cggctcgagg acatgtcgag
     4861 cttcgccaag tatgtcggtg gctgcccgac gaacatcgcc atcggcacgg ccaagctcgg
     4921 cctgcactcc gggctgatca ccgctgtcgg cgacgagcac atgggccggt tcgtcaagga
     4981 ggaggtcgag cgctgggggg tcgacaccgg ccgcctcaag accgaccccg cgcgcctcac
     5041 ctcgctcgtc atcctcggca ttcgcgacga gcacagcttt cccttgatct tcttccgtga
     5101 gaactgcgcg gattcggcgc tggacgaggg cgacatcgac gaagcctata tcgccggggc
     5161 gaaggccgtc gtcgtcaccg gcacgcattt ctccaggccc aatctcgacc gcgcctcgcg
     5221 caaggcggcg accgtcgccc gggcgaaggg tcgcaaggtg atcctcgaca tcgattaccg
     5281 gccggtgctc tggggcctca ccgggcacgg cctcggcgag gagcgcttcg tcgagagcgg
     5341 caacgtctcg gcccatctcc agaccatcct gccgctctgc gacgtcgtcg tcggcaccga
     5401 ggaggagctg cacatcgccg gcggctcgac cgacacgctg gccgccatcc gcaccgtgcg
     5461 cgagcacgcg cccgatgcgc tgatcgtctg caagcggggg ccgatgggct gcgtcgcctt
     5521 tccgggcgcc attccggacg acatcgaaga ggggatcaag gggccgggct ttccggtcga
     5581 cgtctacaac gtgctgggtg ccggcgacgc cttcatgagc ggcttcctgc ggggctacct
     5641 gcgcgacctg ccggtcctgg aatgctgccg gttggccaat gcctgcggcg ccttcgccgt
     5701 ctcgcgccac ggctgcgcgc cggccattcc ctcctggatc gagctcagcc acttcctcga
     5761 gcacggctcg cccgagcggg cgctgaggaa ggaccgcacg ctcgagcagc tgcactggag
     5821 caccaaccgc gagcggcact tcccgcaggt gctggccttc gccttcgacc accgcagcca
     5881 gctcgaggcg atcgccgacg aggcgggggt cagccggccg catatcgccc acttcaagtc
     5941 cttgtgctgg ctggcggtgg agcggcagcg gcagggccgg agcgatctcg gcatcctgct
     6001 cgacggccgc tatggcggcg ccacgctgcc gcgggcgacc ggccagggca tctggatcgg
     6061 ccggccgatc gagcagccgg gctcccggcc gctgcgcttc gacgacgggc ccgacctcgg
     6121 gcgggtgttg cgcgaatggc cgcgcgagca ttgcgtcaag tgcctcgcct tctaccaccc
     6181 ggacgatccg gcggagctgc gggagctcca ggtcgagcgg ctgcagatcc tccaggacgc
     6241 ggcccgcggc accttccacg acctgctgat cgagatcatc gccagcaagt cgggcctgcc
     6301 ggaggaaacg acgacgctcg cccgcgcgat ccgccagctc tacggggccg ggcttttccc
     6361 ggactggtgg aagctgccgg atcccggcag cgacgcggcc tggcgggccg tcgccgacac
     6421 cgtgggcgcc tacgacccgc attgccgggg catcctgctg ctcggcctcg aggcacccga
     6481 ggcgaccctc gaggaggcct tccggcgggc cgccgatcag ccgatctgca agggcttcgc
     6541 cgtcggccgg agcatcttcg gctcggccgc gctcgactgg ttttcaggga agatcgacga
     6601 cgctgcggcc atcgaccgga tggccgccac ctatggacga ctgatcgagg cctgggaccg
     6661 ggcccgtgcc ggaggaagca catgagccgt ctgctgttga agaaccatgt ccccgacgcc
     6721 gccgggctgg tccacgaggt gacgcccgaa agcgccggct ggggccatgt cggcttcgcc
     6781 ctgcaccgcc tcgccaaagg ccagcggctg gaggtcaagg gcagcgccga cgagacctgc
     6841 atcgttctcg tcaccggcag cgccacggtc gaggccgggc cgcagatctt ccgctcgatc
     6901 ggcggccgcg ccagcccctt cgagggcaag ccgaccgcgg tctacgtgcc gcgcgacacg
     6961 gcctttgcgg tgatcgccga gagcgagctc gagctggcgc tctgcacggc accgggcggc
     7021 ggcgagcacg gtgtccggct gatcggcccg gacgacgtcg cctacagcag gcgcggccag
     7081 gggaccaaca cgcgccacgt ccacgacatc ctgccggaga ccgagccggc gcattcgctc
     7141 ctcgtcgtcg aggtggtgac cccggcggga ggctggtcgt cctatcccag ccacaagcac
     7201 gacaccgaca acctgccgca cgaatccctg ctcgaggaga tctactacca ccgcttccag
     7261 ccgccccagg gcttcggctt ccagcgcgtc tacaccgacg accgctcgct cgacgagacg
     7321 atgaccatcg aggacggcga tctcgtcatg gtgcccaagg gctaccaccc ctgcggcacc
     7381 gcgcacggct acgacctcta ttacctcaac gtcatggccg gcccgaagcg cgcctggcgg
     7441 atcaacaccg aggagtgcca ccgctggctt ctaacctgag actcaccgcc gccagaaatc
     7501 cggggtgaag agcaggagca cggtgaagat ctcgaggcgg ccgagcatca tggcgagggc
     7561 caggagccat ttggccgcgt cgggcaggct ggcgaagttg ccggcggggc cgatgatcgg
     7621 gccgaggccg gggccgacat tggtgatggc cgtggcgctg gcggtgagcg ccgtcaccag
     7681 gtcgaggccc atcaggccga gcagcacggt gaagatggcg accatgccga gcagggcggc
     7741 gacgaaggcg agcaccgcac ccggcacgtc ctcgggcacc cgactgcccc catagcgcag
     7801 cggcacgacc tggtgctggg ccacgagacg gcgcagcttg aggcggatga gctgccagat
     7861 gatcaggtgg cggtagacct tgatcgcacc cgaggtcgag ccggcgcagc cgccgaggaa
     7921 catgaggacg aagaagacgg cttccgcacc cggcccccag agcgtgtagt cggtgctggc
     7981 gaagccggtg gtcgtcacca ccgagacgac gttgaaggcg gtgtgctgca gcgcctcgtc
     8041 gaacgccatc tcccgggtga cggcgagcca ggcggccttg ctgccgatga ccaggaagaa
     8101 gaagacgaag agtgctttga cctgcgtgtc cctcagcagg gcaccgcggt cgccgcgcag
     8161 gacccggatg taggccagga agggcaaggc gccggcgatc atgaagatcg tgccgatcca
     8221 gtgcgtcagc gggcgctgga aatagccgaa gctcaggtcg tgggtcgagt agccgccggt
     8281 ggagagtgtc gccatggcgt ggttgacggc atcgaagacg ctcatctcgg cgagcgcgta
     8341 ggccacggcg caggcgatgg tgaggccgag atagacgagg ccgatggatg ccaccatgtg
     8401 gaaggcgcgc ggcaccacct tctccgactg ctccgagctc tccgtgcgga agagctgcat
     8461 gccgccgatc cgcaggaacg gcagcagcac gatcgccatg acgatgatgc cgatgccgcc
     8521 gagccattgc agcagcgagc gccagagcag gatgccgggc ggcaggccgt ccaggccggc
     8581 aaggaccgtc gagccggtcg tggtcaggcc ggacatcgcc tcgaagaagg cgtccgtgta
     8641 gccgtggccg aggccgatca tcggcagggc ggcgaaggcg ggcacgacca cccaggtcag
     8701 cgcggtcagg aggaaggcct ggcgcaggtc gagctggaac gcccccggcc gcgaggtgac
     8761 caggatcagc atgccgccga agaagcccgc gatcgccgcc gagacgacga aggagagcca
     8821 gtccgggttg cccgcggtca cgtcgaccaa ggccggcacc agcatcacca gggccagcac
     8881 cccgaggagg agcccgatca cccgcgcgac cggaccgagc tcgatcatgg cggtgcccgg
     8941 gctcttgcta gcggaccacc cagacggagc agccggcctg cagcgccacc tgcgccgcgt
     9001 tcgagtggtc gagcgattcg tccgcctgcg gcatgtgcgt gcccatgacg atcagatcgg
     9061 cccccacctc agcggcggcg tggcagatct cgcgcaccgg gtcgccccag tcggcgcgga
     9121 catgcgcctc gacggtgatg tcgtcggcga agcgcccggc gacgaagtcg tgcagcgccg
     9181 gcaggtaggt ttccggcagc gtcgccgggt cggcggcggc cggcggcgcc acccagagca
     9241 cggcgagcgt ggcgccgtag cgccgggcca ggtcctcggc ggcgtcgatg gcgtgccgcc
     9301 aggcctcggg gctggtcagg tcgatcggca ggaggatctt ctggggcaag gtccggcgct
     9361 ccgctcaggg gccggtgagg cgctcgccgg ccagggccat catgccacgg ccggcaggcc
     9421 gctgctaccg gacgacgaag accgagatct tggcgtggct cgcgatccag ccggcattgg
     9481 agctgatcag gtgctccttg atgcccggaa tgtgcgaggc catgaccacg agatcggcat
     9541 cgatctcctt gaacgtcttc agcagatcgc gatcgaggtc gaccgccgga tcgaggctga
     9601 ccagcgacct ggccctggcg tcgatgccga gtgcggtacc gcgctcggcg gcgaactgct
     9661 cgagcttgcc cttgaactcc ttcggcgtgt gcgcgacgct cgacggggtc tcggtcgtcg
     9721 cccccacgta atagagcgtg gcaccgtagg ccttggccag gtccgccgcc gtttccagcg
     9781 ccttggtcaa ccggtcgaca tgggcgaggt cgaccggcac ggcaatcacc ttgtacatcg
     9841 aattctcctt gtcccgtccc tagcgcgcca tctgcggatc cgcgcggagc cccttgaaca
     9901 ggcaccagca catcgcgacg aggacgacgg cgaaggggaa gcccgtcgcg atcgccgctc
     9961 cctggagcga ggccagcccg ccgccgagca ggagggcgat ggcgacgagg ccctcgaagg
    10021 tcgcccagaa gacccgctgc gccaccggtg catcggtctt gccgccggcg gtgatggtgt
    10081 cgatgacgag cgagcccgaa tccgaggagg tgacgaagaa gacgatcacc aggacgatgc
    10141 cgaggaagga ggagaccatg gtgagcggca ggacctcgag catcttgaag agcgagagct
    10201 cgggcacgta ggcggcgacc gtctcggcca cgccctggta gttgtcgttc acgatctggt
    10261 cgatggcggt gccgccgaag gcggtcatcc agaggatcga gaccagggtc gggatgatca
    10321 gcacgcaggt gatgaactcg cgcaccgtcc gtccccggct gacccgggcg atgaacatgc
    10381 cgacgaacgg cgaccatgag atccaccagg cccagtagaa ggtcgtccag ccgtgcatga
    10441 aggcggtgtc atcgcgaccg aaggggttgc tgagtggcaa gaggtcggcg gcatagtgcc
    10501 cggcattggc gaagaagccg gtgagcaggg cgatcgtcgg accggcgatg atgacgaaga
    10561 gcagcaaggc gagcgccagc accatgttga tctcggagag ccgcttcacg ccggcctcga
    10621 gcccggcgac gaccgagatc agcgccaccg cggtgatggc gatgatcagc gccaccttgg
    10681 tgacgtcggt cgccggaatg ccgaagaggt actcgagccc ggcggaggcc tgctccgcac
    10741 cgaagccgag cgaggtggcg aggccgaaga gcgtcgcgaa caccgccagc gtgtcgatga
    10801 tgtgcccggg ccagccccag acccggtcgc cgagcagcgg gtagaaggcc gagcggatgg
    10861 tgagcggcag gccgttgttg aaggtgaaga aggcgaggga gagcgccacc accgcataga
    10921 tcgcccaggg atgcaggccc cagtggaaga tggtggcggc catgcccatg gcgcgcgccg
    10981 cctccacgtc ctccgggttc acgcccaggg gcgggttctg gaaatggtag accggctcga
    11041 gcacgccgaa gaacatgagg ccgatgccca tgccggcggc gaacagcatg gcgaaccagc
    11101 cggcatagga gaaatcgggc gtggcgtcgg cgccgccgag cctgatccgc ccccatggcg
    11161 agatgaccag gaagaggcag aacagcacga agatgttggc cgccgacatg aacacccagt
    11221 cgaaggtcga ggtcagccag ggccgcagcc agccgaagaa cgccgcggtg ccctcctgga
    11281 acatcagcgt gaagatcacg aatcccacaa tcgtcaccgc cgagatcatg aagaccggat
    11341 tgtggatatc gagacccagg atctggacgt tgtcttgccc gacctcgtag tcggtcgaat
    11401 cgacagtaaa gtccggttga acgggttcgc tcatcaggca tctccacgac gtaatagggg
    11461 catcgattag gtgccccgcg gcaaggaagc acggaaaagg cgggcaatct tgcccgccac
    11521 cggcagcgcc gaccgataat gctcgggtta ccctaacatg accaaactgc aatgcaaccg
    11581 gtcctgccgg ccatggcttc acccggtccc attcaggcgg ctcgcccgag ctggcgccgg
    11641 gtcagcaggg ggccggcgat ctcgaagaag atggtcgccg ccacggcgac cgcgagcagg
    11701 tcggccgcgg ctgccgggta gcgctccgcc gcgaggagcg ccatgcccat ggcgaccccg
    11761 gcctgcggca ggagcccgag cccgaccgtg gcgaagtcgc ggcggtcggg gccggcgagc
    11821 cagggcaggg ccgcccagcc gcccaggagg cggccggcga cgcgcagggc gaggtaggcg
    11881 agggtcaggg gggccgcggc accgagcctt gccgggtctg cggcggcacc cgcggcgacg
    11941 aagaagaaca cgagaaaggg ccattcgatg tgctcgatct cgctgaacga gcgcgaatgg
    12001 tgcggggtca ggttggcgac cgcgacgccg gcggtcatgc cggcgagcaa gggcgagacg
    12061 gcgagcgcgt cggccaggcc ggcaatcagc aggatcacgg ccaccgcctc cacctgggtc
    12121 ggctcgccgg gccgcagccg gccggtgaga aaggccgccg gtgcgccgat cgcgagcccg
    12181 agcagcagcg cgccgccgag ctcccagccg gccctgccga ccgcgtgcca cccgtccgcc
    12241 ccggcgacca gctcgagcag gctcacggcc acaccgaagg cgatgatgcc ccaggcatcg
    12301 tcgatggcga cgataccgag caccacctgg ccgacccggc cgcccccgcc ggcctccctg
    12361 accacctcgt gcacggcggc cgggtccgtg gcgaccgcca tggcggcgag cggcagtgct
    12421 atggccggca ggaagcccag ggccaggagc ccgccaccga ccaggagcgc gctcaccagg
    12481 gcgatggcga gcgacagcgc caggaccggg cggccggtca gtcgccagcg ctcggcggtg
    12541 agatcgccgc cgaggagaaa cgccaccatg acgagggcga tggacaccag cggctcggcg
    12601 acggcggcgg tgagcaccgt cacctcttgc tcgaggagca cgcgcgccgc ggtggcgatg
    12661 gccaccccgg tgagcaccag gagcgagacc cggggcaggc cgagccgccg cgccagcacg
    12721 tcgaccatga ggctgagggc gagcactgcg ccgagcatca ggaggaccgc cgtgtcggtg
    12781 gcgatcgccg agagggtgaa gagtggctcc gacatcggcc tcgctcggcg atggtcgcgc
    12841 gacgggattg acggcggcgg gcaaccgtgc cgacctgtgg cggcacccga ggcccgacca
    12901 cttgcccggg acccgactac gaaggagagc cgcattgccg gaaattttca accaccactt
    12961 cacccggtcg gagctgaccc ggcatgtcgg cgacacctcg cagcttttcg gcgtcgacct
    13021 cgtcacgcac gaggacgggc cggagcgcgg cctcagggtg ctgcgctttc gcacgggcgg
    13081 cgggctcgag ctcgaggtgc tggtcgaccg ggccatggat gtcggcgcca tgagcgtctg
    13141 cggcgtgccg ctcggctggc gctcgggcaa cggctttcgc cacccctggc tgcacgagca
    13201 cgacgccgag ggcggcctcg gctggctgcg gtcgttctcc ggtctcgtca acacctgcgg
    13261 cctcgaccac atcatgggca tggcggagga gggggcggat cacttcaact acccctaccg
    13321 ggccagggtc gcgcacggca tccacggccg cgtcgcgttc accccggcac ggctgcaggg
    13381 cttcggcgag cgctgggacg gcgacgattg cgtcctccat gccgagggaa ccgtgcgcca
    13441 ggtcacgatg ttcggcgaga acctggagct ggcgcgccgc atcgaggcga cggtcggcgg
    13501 gcgaaccgtc aagatcacgg acagggtgac caatctcggc taccgcacca cgccgcacgc
    13561 gctgctctac cacgtgaatg ccggctggcc ggtggtggcg gccgaatcac ggctcagcct
    13621 gccgctcgag cgcacggtct accatgccca cgacccgcat gcgacgagca tcgggcccct
    13681 ggagcaggcg gccccccagg tcgggttcag ggagcaggtc tacgagcacg cgctcaggcg
    13741 ccgcgcggac ggcatggtgc cggtcctgct cgccaacccg gacttcggcg gcctttcggg
    13801 ctcgcgcggc atcggcctgg ccatgcgcta tgacggccgc agcctgccgg ccttctacca
    13861 gtggcagaac ctgcacgaag gcgactatgt catcggcatc gagccggcga cctgccatgc
    13921 cggcagccgc gaggactgga agaagcgcaa cgagatgatc tggctcgagc acggcgacag
    13981 tcgcgagtac cgcctcgagc tggagggcgt cgtcggcgag gccgccctcg aggcgatggt
    14041 cgacgagatc gatggcctcc gctagggcgg tgctcgacca gccgggcacg cactggcgga
    14101 tcgagacggc cgcgcgtgcg gcgctgctga tcgacgggga tgcttatttc caggcgctcg
    14161 ccgccgccat ggagaaggcc gagcgctcga tcatcatcct cggctgggac ctgcgaagcg
    14221 acctcctgct cgaccccatg aactcgagag agaccctggc cgcccggctg cagcggctga
    14281 tcggggcgcg tgccgggctg catgtgcgga tcctggtctg ggactggccc atcgtgatgg
    14341 gtgcggggcg ggaggtcctg ccgcactgga gcttcggtcc cctggaggag cggctgacat
    14401 tcgtcctcga cgacgagatt ccggtcggcg gcgcgcatca cgagaagctg gtcgtcatcg
    14461 acgaccggct cgccttcgtc ggcggtctcg acctcaccgc cggccgctgg gacacgcccg
    14521 agcaccaccc gggcaacctg cgccggctcg agatcgagag cggcctcgac cgggcgccgt
    14581 tccacgacgt catgctgatg gtcgagggcc cggtcgccgc cgccctcggc gagctcgccg
    14641 ccgagcgatg gcgcgtggcg accgacgatc acctggcgcc ttccggctcg tccgggaccg
    14701 acctctggcc cgaagggatc gaggcggacg cccaagacgt gccagtcgcc atcgcccgaa
    14761 cccggcccga gatcgacggg ctcgaagccg cccgcgagat cgaggcgctc tacctcgccg
    14821 ccatcgccgg ggcagagcgg ctgatctata tcgagaacca gtacctcacc gtgccggctg
    14881 tggcccgggc gctggcccgc cgtttgcgcg acatgccgga cctcgaggtc gtgatcatca
    14941 cacccaaggc ctgcgaaggc ccgctcgaga ccgcggtgat ggatgcgggg cgcaagaagt
    15001 tcaaggcgac gctcgaggcc gccgcccggg accgggtccg cgtgctgacg gcgcagagcc
    15061 acgggcttcg ggtcaatgtc catgccaagc tgatggccgt cgacgatcgc ctgttcaccg
    15121 tcggcagcgc caatctcgcc aaccgctcca tgggcgtcga taccgagacc aacctcgcca
    15181 tcgagcggcc ggagcccgat ccggtcatcc gcggctggcg ccaccggctg ctggccgagc
    15241 atctcggccg cgagcccggc gaggtcgcgg catgcgaggc ggagctcggc tcgaccatcg
    15301 ccatgatcga gcggctctcc gaccacgagg ccgcgcacta tctggcaccg ctcgatctcg
    15361 atgcccaccc gataccggag ccgctctcgg acatcgtcga cgtcgccgag ctcgccgacc
    15421 cgcccgagcc gatcatcggc tcgcagctcc tcgccgagct cggcctgccg agacgctttc
    15481 gcaacctgcg gcgctggctc ggccgggttg ccggcctcgt cggtgtcgcc gtgctgctcg
    15541 cctggttcct cggcgctatc gacctggtca gcggctgggt ggtcgcgggc gccagcctcg
    15601 ccctcgtcct cggctgggcc gctggcgagc gtccctggcg gctggagcga cactgatgcg
    15661 ggttgccacc tacaacatcc atggtgccgt cgggctcgac cggcggcgcc ggcccgagcg
    15721 catcgtcgag gtcatcgaga gcctcgatgc cgacatcatc ggcctgcagg aggtcgaagg
    15781 ccgccgcagc cgcagtcgcg tcgaccaggc gcagcacatc ggcgatgcgc tggactacca
    15841 tctggtcgcc ggcccgctgc tgctcgaggg catgggcggc tatggcaacg tgctgctttc
    15901 gcgccatccc gtgctcgagg tggagcgccg cgtctaccag cggccgggga gccagacccg
    15961 cggcctgctc gacgccgtgg tggcgcaccc gctcctcggc cggctgcggg tcatcgtgac
    16021 gcatctcgag gtgcgcgacc accgcattcg cgcgacgcag ctgcgcgagc tcgagagcct
    16081 cctggccgaa gggccggagg ggccggccgt gctgatgggc gatctcaatg agtggtggag
    16141 ccggcgcgtg gcgctgaagt cgctcgaccg cacggtccgc ttcctccact cgccggccac
    16201 gttcccggcc cggctgccga tcctccgcct cgaccggatc ggggtcaggt cgctgcgctc
    16261 gaccgatccc gacggtcgcg cgcggcgggt ggaaacccgg ctgacgcgca ttgcctcgga
    16321 ccatctgccg ctggtcgccg acctcgagcc gctcgaggcg ctgccggcgc cggaggacga
    16381 ggatcaggcg agcagcactt cctcccggca ttccacgccg ctggcccggc aataggcctc
    16441 gagctcggcg aaggcggcgt cgatcgccgg cacgtcgtcg ccgcgaaaga cgatgctggt
    16501 gccgggcttg tccatccgga agaacgggta gctgccgatg tcgatcgccg gatagcggcc
    16561 ctggatggcg ccgagcaccg ccgccatgtc gccctcgccc ttgaagacga cgagggtgcg
    16621 cgagtgcacc agcgccccgc cgaccagccg cggcttcagg atgtcgagca tcgcctgcat
    16681 gatcttcggc acgccggcca ggacatgcac gttctcgacg atgaagccag gggccacgct
    16741 gaccgggttg tcgatcagga ccgcaccctc cggcgtctcg gccatcttga ggcgcgcctc
    16801 ggtcaccttc tccggcgggt agaacgcccg cagccgccgc tcggcctcgg gatgccggat
    16861 gagcttctgg ccgaacgccc tggcgatgca gccggcggtg atgtcgtcgt gggtcgggcc
    16921 gatgccgccc gtggtgaaga ggtagtccac cttgacgcgc atggcgttga ccgcgtcgac
    16981 gatctccctt tcgatatcgg ggatcacccg ggcctcgacc agcctgatgc cgatgccgcc
    17041 caggaaggtc gcgagatagg ggaggttggc atcgaccgtg cggccggaga ggatctcgtt
    17101 gccgatgatg agcaggcccg ccgtcacctt gccttttttt tgctcgccgt ccgttgccat
    17161 tcttcacgcc tcccctgctg cctgacccgg atatagcgcc cttggacctg cccgtccccc
    17221 tgcatcgcgc cacgctgcgg caccgctaca agcgctttct cgccgatgtg accatggccg
    17281 acgggcgcga gctcaccgcc cacatcgccg accccggccg cttgcccggc ctcgccgtgc
    17341 cgggcgccga tctctggctg tcgcggagcg actcgccgaa gcgcaagctc gcccaccgcg
    17401 tcgagctggt ggagacgccg gagagcctcg tcgggctcga cacgggcaat gccaaccggc
    17461 tcgtcgccga ggcgctggcc ggcggccggc tgcccggcct cgaagactgg caggcgaccg
    17521 cccgcgagcc ccggctccac gccggcacgc ggctcgattt cgcgcttcgc gcgagtggcg
    17581 ggcgaagcgc cttcctcgag gtcaagaacg cgacctggcg gcgcggtgcc gtcgccgcct
    17641 tccccgacgc cgtcaccgcc cgcggcacgc gccatctcca ggcgctcgcc gggctcgccg
    17701 ccgccggcac gccggccttc ctgctcttcg tctgccagcg ctcggacgtt gccgccctca
    17761 ccatcgccgc cgacatcgac ccggcctacg cggccgcctt cgagctggcc cgggcggccg
    17821 gtgtcgccat actcgcctgt cgttgcaaac tcaaccattg cgcgataaca atcacggagg
    17881 gcatcc
//
LOCUS       DAOTFI010000094        17867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3795_length_17867_cov_7.154727, whole genome shotgun sequence.
ACCESSION   DAOTFI010000094 DAOTFI010000000
VERSION     DAOTFI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17867
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_17867_cov_7.154727"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="P5558_11215"
     CDS             complement(<1..235)
                     /locus_tag="P5558_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paraquat-inducible protein B"
                     /protein_id="HRY24924.1"
                     /translation="MPPVPPDRPIAEPVLRKSRGFSLVWIVPIVAAAVGIWLAVTTVM
                     NRGPLVSVTFVTAEGLEAGKTKVKYRDVDVGTVE"
     gene            complement(216..1466)
                     /locus_tag="P5558_11220"
     CDS             complement(216..1466)
                     /locus_tag="P5558_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HRY24925.1"
                     /translation="MATDRPALICEECDTLTAVRRVPSGVTAQCSVCGNVLYRERQDS
                     VRRALALTLAAFILLLIAHAFPFMTFTMEGNSTSGTIFGSVMALWHDGYWELAIVVLT
                     MASLMPFIRLALGLWVLVPLTMGRVAPGTFELFGLYSRFQAWAMIEVYLIGVLVAYTK
                     LVDLASINLGPAMWAFGGLIVCAAAADNVLDPRAVWWRLAPQNKLGALTATGQPLVGC
                     HVCGQVVPLPVDAHHVHCPRCHAALHHRKPDPLARTWALLIAAALLYIPANVLPVMTV
                     TSFGKGQPDTILSGVIELIHADMWPIAALVFFASITVPVLKILGLVLLLVAAQRGWVA
                     RARDQTRLYRVIEQVGRWSMIDIFMISILAVLVNLGEIAQIEPGPGAIAFASVVILTM
                     LAAHSFEPRIIWDNRRRNHATSPA"
     gene            1636..3906
                     /locus_tag="P5558_11225"
     CDS             1636..3906
                     /locus_tag="P5558_11225"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HRY24926.1"
                     /translation="MLSEMSPGHSWMAYWLDATQRSILTLDVLRQRGNQFVEHYQAGK
                     PPVLVFEHELVLDGKDLDRPSNYALLRILPEAEVPTDPKKRPFVVFDPRAGHGPGIGG
                     SKEASQVGVALRAGHPVYFVAFRPEPVPGQTLADVAYAEWCFLQKVQELHPEAEAKPA
                     IVGNCQAGWAIMILAAYAPDQASVIGVSGAPLSYWAGVEGKNPLRYLGGLLGGNWLSS
                     LASDLGAGTFDGVNLVSNFENLNPANTYLRKPYNLYSKIDTEAPRYLGFERWWGGFFM
                     LTQDEIEAITSELFVGNKLTQGKLVAPDGTRIDLRAIRAPIVVVCSHGDNITPPQQAL
                     NWILDLYDSVDEIRANEQTIIYTVHPSVGHLGIFVSAKVALKEHAEFVDSLDLIETLA
                     PGLYEMVITETHLEDAERPTAHQEYEVRFEARTLNDIRALDDGREDEVPFRTLAKVSE
                     TNQGLYRQLVSPWLRAVVTPQTAEALRLMHPHRQSQLWLSDLNPWMAGVKWLAEQTRE
                     SRAAAPADNLWRAMEAANVDQLEAAIERWTDARDRAVEQFFKAIWTQPFIRALVGEEA
                     SHADVKKPRARQDKAFHELAALKLAAFANRQKEGSFAEAILRVVYAATKATGGVDARA
                     FVAARAVWQSHPRLAALGRERFLAEAKEAALMVAFDEERALTTLPELLPTFADREEAL
                     TVLRQIIDRRADVLPEVVALMKRVETILEAQSVQGSDNPAPAPAPRPKPAAKVQELRA
                     PAPRRPATHRTTKTTS"
     gene            4064..5212
                     /locus_tag="P5558_11230"
     CDS             4064..5212
                     /locus_tag="P5558_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY24927.1"
                     /translation="MTEQPVRSAPRLDRRRFLQASGVGAAAIAAPAIVSPKALASSGE
                     INIMMWSDYLPDSFRESFTQATGITINFTGIGSNEEIINRMRASRGQGFDIISPTMNR
                     APQWAEIDLLQPLDLSRIPTDKVNPNMLAQGSQIWNLNDKGPSWVPHIWGTEGIAWRT
                     DLWQPEGEFPSYGAVWEDANAGKTMGRGHSMMLGAGLYLERIGELEPGSIWLAYTNED
                     DMRRVWGRLTEFCISKKPNVRIIWNDADTQKNGLMNEGVIVGQTWDGPPLSLKTAGEP
                     VMYRAPIEGAMAWVDGMSMPIGAENIDQAYAFIEHCYDAEAAGRAIDTHGYNSPVIGA
                     ERYANEDYARNFADAYPGDALANLNPWPAELPWYADIRAEYVNRFLAA"
     gene            5309..6421
                     /locus_tag="P5558_11235"
     CDS             5309..6421
                     /locus_tag="P5558_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY24928.1"
                     /translation="MAGGVSVELDRLLVQFDEFVAVRDANVRIEPGEFFSFLGPSGCG
                     KTTILRTISGFLEPTSGAVRIDGKDMAFIGPNKRPTALIFQNLALFPLMTVWENIAFA
                     LEMKGVGAADRRKHADELLALIALDGQGDKYVHELSGGQKQRVAIARALCAEPSVLLL
                     DEPLSALDLKLRQHMRTELRTIQRRVGLTFIYITHDQGEALTMSDHVAVMREGVIEQI
                     GDGRTIYDDPATAFVASFVGENNALPGRVAEIRDGVALVDTSVGRLRARIATAARDRL
                     RTDEEAIVFIRPEALDLDIAGASAETTIQATVIGEEFEGPNMHLQLRHDGGAELRVSL
                     VNKGRTGTAGIGSSLAAAFDPMKAVALPSGALSAEE"
     gene            6429..7358
                     /locus_tag="P5558_11240"
     CDS             6429..7358
                     /locus_tag="P5558_11240"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24929.1"
                     /translation="MAKRSWLGGFLDRNGQALGLYILLAVSFWLLFLIILPQLAMIDF
                     SLRHDLPAAQQGGPQDVWTFENYRHFIFGLPGNPDGWNRLDVGVFGRTIVAAAFVTIL
                     NLAICYPVAYYLAQVARGRQARVMAVALVVPYWVNEILRAFAFRIIFGATGVINSTLL
                     TLGIIDLPIDFIRADIALYAGLGYAYVLLMVFPLYNAIESLDRNQIEAARDLGAPWWR
                     IHTRIVLPFAKPGITSGCTMVFMLAAGALAAPQILGGPSNLWFTQLIYGQFFESVNWP
                     RGAAYAIILLVACTLLVLLVMRLFKVRLGEIGK"
     gene            7355..8632
                     /locus_tag="P5558_11245"
     CDS             7355..8632
                     /locus_tag="P5558_11245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY24930.1"
                     /translation="MTGRRSPRWSQWVIGAYLLLFFAYLFGPLLIMAVTAFNSSSFPR
                     VSPWECLTFEWFVRLVEDQRLMQGLRNSLIIAFGVVVLSVAIGLAGALFLTQIAARSR
                     AGTYTVFISPILMPGVVLGIATVLFWDRVAGSIGMAYGGLFYDGIFLTVLGQSCFIAS
                     YCLLVFLARLQRFDSQLIDAALDLGATNTQAFRRILLPFLKPAIGGAAVIAFLASFEN
                     YNTTVFTISFYNTFTVEVAQKVRLGIDPTISALAVIVIAISLVAALANEAGQSHQQRR
                     LEGHGRGFVAFFTRNPAAVLAALAVGVIVVVLAFAVQHDPAACKARALEQRLERQQQY
                     QPATPAPAAPTQSAPAAPGARDLNPGQGSFGDVFRGGLAPGSTPTPEPTPAPTPDTRS
                     LNPGQGMFGDVFGGGLAPGTTPAPESHEGDSEE"
     gene            complement(8645..11089)
                     /locus_tag="P5558_11250"
     CDS             complement(8645..11089)
                     /locus_tag="P5558_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY24931.1"
                     /translation="MTDLPSSAAVVVVGGGIVGCSTAYHLAAMGVGEVLLLERDKLTS
                     GSTWHAAGLVGQLRTNANITRLLGYSVELYDRLEAETGLATGWQRNGGLRLACNADRW
                     TEVRRQATTAHSFGLEMHLLSAKEAQALWPLMQVDDVVGAAFLPTDGQANPSDIAQAL
                     AKGARTKGVRIVEDVAVTGFVVEQGCIRAVETDHGRVACGKLVLACGQWTRQLAARLG
                     VNVPLVSVQHQYVITEPIAGVTPDLPTLRDPDRLTYYKEEVGGLVMGGYEPNPIPWAL
                     GGVPERFAHQFLDEDWEHFEPIMELALGRVPALAEAGIKTMFNGPESFTADGNFILGE
                     APEVRNVFVGAGFNAFGIAAGGGAGMALAEWVARSAPPFDLWAVDIRRFGAAHRDTDW
                     VRTRTLEAYAKHYTMAWPQEEMSSGRPLRRSPLYDRLEGAGAVFGEKLGWERANWFAG
                     PGEVAQDRYGFGRQNWFAAVGREHRAAREAAALFDQSSFAKFELAGPDAEAALSWLCS
                     NDVAKPVGSLTYTQMLNERGGIECDLTVARLESDRFYIVTGTGFATHDRDWILRHIPA
                     GMDVRLTDVTGAHAVLSLFGPKAREVLQGLTRADLGNAAFAFGAVREIGIAGCPVRAL
                     RVTYVGELGWELHVPVDFALTLFEALREAGQPHGLILAGYRAIESLRLEKAYRAWGSD
                     IGPDHTPLMAGLGWAVKLRKEIPFLGREALLAQRQQNLPRLLAGFEVADPAVVLLGRE
                     TIYRNGERAGWLSSAGWGYTIEKNLGLGYVRREAGVDRAYVLDGTYELEVAGERVSAE
                     VFLDPPYDPTNARVRA"
     gene            complement(11086..11973)
                     /locus_tag="P5558_11255"
     CDS             complement(11086..11973)
                     /locus_tag="P5558_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRY24932.1"
                     /translation="MTGDATARAAALPIWKGPVEPRPLAGGITNTNFTVQDGGRRYVV
                     RVGGDIPLHGVLRFNERAASEAAAAAGISPAVIHAEPGVLVLAFVEGRTLTPADVRER
                     AMQTRILPLLRRVHCEMPDHLRGPVLAFWVFHVIRDYAHTIRDGGHRLAPLLDGWLAT
                     ATRLERAVGRIDLVLGHNDLLAANFIDDGKRIWLVDWDYAGFNSPLFDLGGLASNSEL
                     AEADRLWLLEAYFDRPIDDELARQSRAMLTASLLREAMWSMVSELHSAIEFDFKAYTE
                     ENLARFERSLEAFDAMERP"
     gene            complement(11970..12401)
                     /locus_tag="P5558_11260"
     CDS             complement(11970..12401)
                     /locus_tag="P5558_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY24933.1"
                     /translation="MPVVMLFRVGDFAAMIAIVLYALAPAIRYARHSVARTPAELVEA
                     GTAAGCTERQILTRIRLPLAVPGILLGLNQIIMLALSMLVITALVGTHDLGQDVYIAL
                     TKADVGLGLVAGLCVAAIAIIADRLIAAASLRMRHRLGLEA"
     gene            13157..14038
                     /locus_tag="P5558_11265"
     CDS             13157..14038
                     /locus_tag="P5558_11265"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY24934.1"
                     /translation="MTKATPRIAFIGLGQMGSRMAARLAEAGLPLAVHDADAGAMEPF
                     GAERSHPSPKAAAAGCDVLLTMLPNGRVAQEAVLAADPAPGVLVVDMSSSDPADTQAM
                     GAALADLGVALVDAPVSGGIKRAATGELAIMAGGEARDLERARPVLEHLGRRIFHCGP
                     LGAGGAMKALNNLLSAAGFAATIEVLQIGRRFGLDPELMLDVLNNSTGRNNTTEVKIT
                     PFVLSGRFDSGFALDLMVKDLTTALALAGRTETDVPLGRLALDLWREAGGDLEAGVDH
                     TALARWYEKRTGTQLRS"
     gene            complement(14062..15276)
                     /locus_tag="P5558_11270"
     CDS             complement(14062..15276)
                     /locus_tag="P5558_11270"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24935.1"
                     /translation="MTEPLPDVRRRLGVRPLINLTGTLTTHGGISACPEAIEAAANIM
                     SRGVDVLELQAAASRIIARATGAEAGFVAACSSAGICMGVAGAMTGDDLARIERLPDT
                     TGMRSEVVIQAGHVVNYGHSIAQDIRLPGARPVVIGDVNAARSHQLAAAIGPQTAAGL
                     FVVSHHCSGEGQIPFATFVEICHVKGVPVIVDLAAEYDLRGYLAHGADLTIHSSHKFL
                     GGATAGIVAGRKPLVRAAYLQNFGIARPMKAGKESVAGALAALEAWGTRDHAARRQGM
                     REVLEDWRRRLGALPGIEATIDPDPTGNPFDRLKLEVDPIEAGLNALQLVRELERGDP
                     PIAVRSHQVEHGWLVMDPRSLGEGEAEIVADRIGAVLASAGRDGLGPASSDLESWRAE
                     KARALRLWPDSD"
     gene            complement(15273..16757)
                     /locus_tag="P5558_11275"
     CDS             complement(15273..16757)
                     /locus_tag="P5558_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRY24936.1"
                     /translation="MRRILVLQCEQEISSFNPVPTEYESFSIAMGEALIEANRQADTC
                     VRGALDVLGARSDVTVVPVYGAKAPSGGVLRADAFRRIKGDLLAALREHAQGADGLYF
                     SLHGAMGAEGELDPEGHLLEAARGILGPDVPIVTSLDLHGILTARMLRNMTATAVYHT
                     YPHEDFIDTGARAARLLLRIIDTGARPVMARVKVPALVRGPELFTKSGCYGEIIRNAK
                     RLEANVALAAAMLIGNPFTDVPELCSQSLVITDGDVDAAKREALAMAVGFWRQRERMR
                     APLVSLDGAIEAAKTATAPVTFTDAADAPSSGASGDSNAILKGLLQRGYPGRVLMPIV
                     DAPAVERAHAAGVGARLQLAIGGSLDPERFPPLPVEVRVAMLGEGRWRHEVNGLMANA
                     GRTAVLEADNITLIVISRPVHLMDRGVFLAHGHDPRDFDLIVIKSPGAYSRFFTFAEE
                     NFVVDVPGATSANLPLLGHKVCARPMFPLDPDVVFEPEVEVYPA"
     gene            17174..>17867
                     /locus_tag="P5558_11280"
     CDS             17174..>17867
                     /locus_tag="P5558_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY24937.1"
                     /translation="MLEPIADQFQPSALRSVYMLNGETGERSYYAIPWTQMTPHVHYW
                     KDLLVQPGFTEDDIPNGWQPFFDFWCDEVQPALRAKGERVYGLGLGTGSGGNDPFFNI
                     HIFLNVFGAEVLDADGNLQIDDPDVRARVIEAVKSFVKPSAEQCVPPDGVNCAGADDI
                     VYFINRKNLVAFNPTLSIPATQLKDNPENYYENMRTRPWPKGVESEPAPAMVSVKQVL
                     IFEVSPNKENAKD"
BASE COUNT         3045 a         6114 c         5872 g         2836 t
ORIGIN      
        1 cctcgaccgt gccgacgtcg acgtcccggt acttgacctt ggtcttgccg gcttcgagcc
       61 cttccgcggt gacgaacgtc acggaaacca gtggccccct gttcatgacg gtcgtaacgg
      121 cgagccagat gccgaccgcc gccgcgacga tcggcacgat ccagacgagg gagaagccgc
      181 gactcttgcg cagcacgggc tcggcgatgg gtcggtcagg cgggactggt ggcatgattc
      241 cttcgcctgt tgtcccagat gattcgcggc tcgaagctgt gtgcggcgag catggtcagg
      301 atgaccacgg aggcgaaagc gatcgccccg ggtcccggct cgatctgcgc aatctcgccg
      361 aggttcacga gaacggcaag aatcgagatc atgaaaatgt cgatcatcga ccagcggccg
      421 acctgctcga tcacgcggta gagccgtgtc tggtcgcggg cccgcgcgac ccagccgcgc
      481 tgcgccgcga cgaggagcag gacgaggccg aggatcttga gcacaggcac ggtgatgctg
      541 gcgaagaaga cgagcgccgc gatcggccac atgtcggcgt ggatgagctc gatgacgccc
      601 gaaagtatcg tgtccggctg ccctttgccg aagctggtga cggtcatcac cgggagaaca
      661 ttcgccggga tgtagagcaa agctgcggcg atgagcagcg cccaggtgcg cgcaagcggg
      721 tccggcttgc gatggtgcag ggcggcgtgg cagcgcgggc aatgcacgtg gtgggcgtcg
      781 accggcaacg gcacgacctg gccacaaacg tggcaaccga cgagaggctg gcccgtggcg
      841 gtcagggcac ccagcttgtt ctgcggcgcc agccgccacc agacggcgcg tggatccagc
      901 acgttgtccg ccgcggcagc gcagacgatc aggccaccga aggcccacat cgccgggccg
      961 aggttgatgg acgccagatc gaccagcttg gtgtaggcga cgagcacgcc gatcagatag
     1021 acctcgatca tcgcccaggc ttggaatcgc gagtacaggc cgaagagctc gaacgtcccc
     1081 ggcgctaccc ggcccatggt cagcgggacg agcacccaga gcccgagcgc gagccggatg
     1141 aacggcatga gactggccat cgtgaggacc acgatggcga gctcccagta cccgtcatgc
     1201 cagagcgcca tgacgctgcc gaagatcgtg cccgaggtcg agttgccctc catcgtgaac
     1261 gtcatgaacg ggaaggcgtg ggcgatcagc agcagaatga acgcggccag ggtgagcgcc
     1321 aatgcgcggc ggacgctgtc ttgccgctcg cgatagagca catttccaca aacgctacac
     1381 tgcgcggtca cgccggacgg cacacgccgg accgcggtca gggtatcaca ttcctcgcag
     1441 atcagggcgg ggcggtcggt tgccatgcat tcagcctagc ccgcagcatg gccacagcgc
     1501 gcggcttttc tgcatcggtt ggccgggccg gccgccgacg ggcgagggct gagacgattt
     1561 ggccgcgccc gccgaacttg cggcgcaccg cacagcagct atgttgcagt gcaacaatat
     1621 agtgggagat ggccgatgct gtcggagatg tcgccgggtc attcatggat ggcctactgg
     1681 ctggatgcca cccagcgctc gatcctcacc ctcgacgtgc tgcgtcaacg cggcaatcag
     1741 ttcgtcgagc actaccaagc cggtaagccg ccagtcctgg tcttcgagca cgagctggtc
     1801 ctcgacggca aggatcttga ccggccgtca aactacgcgc tcctgcggat cctgcccgag
     1861 gccgaggtgc cgaccgatcc gaagaagcgg ccgttcgtcg tcttcgatcc tcgcgccgga
     1921 catggcccag gtattggcgg ctcgaaggag gccagccagg tcggcgtggc gctgcgggcc
     1981 ggccatccgg tctacttcgt cgcttttcgc ccggagcccg ttccgggcca gaccctggcc
     2041 gacgtggcat acgccgagtg gtgctttctc cagaaggtac aggaactgca cccagaggcc
     2101 gaggccaagc ccgccatcgt cggcaactgt caggccggct gggcgatcat gatcctcgcc
     2161 gcctacgcgc ccgaccaggc gtcggtgatc ggcgtctcgg gcgctccgct ctcctactgg
     2221 gccggcgtcg agggcaagaa cccgctgcgc tatctgggtg ggcttctggg cggcaactgg
     2281 ctttcgtcgc tcgcttccga tctcggtgcc ggaaccttcg acggcgtcaa tctcgtcagc
     2341 aatttcgaga acctgaaccc ggccaacacc taccttcgca agccttacaa cctgtactcg
     2401 aagatcgaca ccgaggcgcc gcgttatctc ggcttcgagc ggtggtgggg tggctttttc
     2461 atgctgaccc aggacgagat cgaggcgatc acgtccgagc tgtttgtcgg caacaagttg
     2521 acccagggca agctcgtcgc acccgatggc acgcgcatcg acctgcgcgc cattcgcgcg
     2581 ccgatcgtcg tcgtctgctc gcacggcgac aacattacgc cgccgcagca ggcgctcaac
     2641 tggatcctgg acctttacga cagtgtcgac gagatccgcg ccaacgagca gaccatcatc
     2701 tacacggtgc atccctccgt gggccatctc ggcatcttcg tctcggccaa ggtggcgctg
     2761 aaggagcatg ccgagttcgt cgattcgctg gacctgatcg agacgctggc gcccggcctc
     2821 tacgagatgg tgatcaccga gacgcatctc gaggatgccg agaggccgac ggcgcatcag
     2881 gaatacgagg tccgtttcga ggcgcgcacg ctgaacgaca tccgcgccct ggacgatggg
     2941 cgcgaggacg aggtgccgtt ccgcacgctc gccaaggtct cggagaccaa tcagggcctc
     3001 tatcgccagc tggtgagccc atggcttcgg gcggtggtca ccccgcagac cgccgaagcc
     3061 ctgcggctca tgcatccgca ccgccagtcc cagctctggc tttccgacct caatccctgg
     3121 atggccggcg tcaaatggct ggccgagcag acccgggaga gccgggcggc agcgccagcg
     3181 gacaatctct ggcgggcaat ggaggcggcg aacgtcgatc agctcgaggc ggcgatcgag
     3241 cgttggaccg atgcacgcga tcgcgctgtc gagcagtttt tcaaggctat ctggacccag
     3301 cccttcatca gggcgctggt cggcgaggag gcatcgcacg ccgacgtcaa gaagccgcgg
     3361 gcgcgccagg acaaggcctt ccacgagctc gccgcgctca agctcgcagc cttcgccaac
     3421 cgccagaagg aaggcagctt cgccgaggcc atcctgcgcg tcgtctatgc cgcaaccaag
     3481 gcgacgggtg gtgtggatgc cagggctttc gttgccgccc gggccgtctg gcagagccat
     3541 ccgcgcctcg cggcgctggg tcgcgagcgc ttcctggccg aggccaagga ggcggcgctg
     3601 atggtggcat tcgacgagga gcgggccctg accaccctgc ccgagctgct gccgacattt
     3661 gctgaccgcg aggaggcgtt gacggtgctg cgccagatca tcgatcgacg tgcagacgtg
     3721 ctgcccgagg ttgtggcgct catgaagcgg gtcgaaacga tcctcgaggc acagtcggtc
     3781 caggggagcg acaatccggc accggctcct gcgccccgcc cgaagccggc tgccaaggtg
     3841 caagagctgc gagcaccagc ccctcgccgc ccggcaacac atcggaccac caagacgacg
     3901 tcatagaagg agccgctgcg ccgtcagtga tgcgacagtt ctgaccaccg tacccggtta
     3961 ccagcaatgc gttcgcgctt gcacgatccc ggcacagcgc actacgcttc gggcaaggag
     4021 ccatgagcac ggcccacaac aagtaatcag gagggatacc aagatgaccg agcagcccgt
     4081 acggtcggcg ccaaggctcg accgccgccg cttcctgcaa gcttccggcg ttggcgccgc
     4141 ggcgattgcg gcaccggcga tcgtcagccc gaaggcgttg gcctcgtcgg gcgagatcaa
     4201 catcatgatg tggtcggact acctgccgga cagcttccgc gagagcttta ctcaggccac
     4261 cggcatcacc atcaacttca ccggcatcgg ctccaacgag gaaatcatca accggatgcg
     4321 agcatcccgt ggccagggct tcgacatcat cagcccgacc atgaaccgcg ctccgcaatg
     4381 ggcggagata gatctcttgc agccgctcga cctcagtcgg ataccgaccg acaaggtcaa
     4441 cccgaacatg ctggcgcagg gcagccagat ctggaacctc aacgacaagg gaccgtcctg
     4501 ggtgccgcac atctggggca ccgagggcat cgcctggcgc accgatctct ggcagcccga
     4561 gggtgagttt ccgagctatg gcgccgtctg ggaagatgcc aatgccggca agaccatggg
     4621 ccgcggccac tcgatgatgc tcggcgccgg cctctatctc gaacggatcg gcgagctcga
     4681 gcccggctcg atatggcttg cctacaccaa cgaggacgac atgcgccggg tgtggggcag
     4741 gctcaccgag ttctgcatct cgaagaagcc gaatgtccgc atcatctgga acgacgccga
     4801 tacccagaag aacggcctga tgaacgaggg tgtcatcgtc ggccagacct gggacggtcc
     4861 gcccttgtcg ctcaagaccg ctggcgagcc ggtcatgtac cgggccccga tcgaaggtgc
     4921 catggcctgg gtcgatggca tgtcgatgcc gatcggtgcc gagaacatcg accaagccta
     4981 cgccttcatc gagcattgct acgatgccga ggcggcgggt cgcgccatcg atacgcacgg
     5041 ctacaactcg ccggtgatcg gcgccgagcg ctacgccaac gaggactatg cccggaactt
     5101 cgccgatgcc tatccgggcg atgccttggc caacctcaac ccgtggccgg ccgagctgcc
     5161 ctggtacgcc gacatccggg cggaatacgt taaccgtttc ctggcagcct gagccgatcg
     5221 gaggcatcgg cctggcggtg cctccgattt ccggcacgac ttcgctcacc gaccgtcggt
     5281 gggtgaacag cgcgagcgga gcggacgcat ggcgggtggc gtgagcgtcg aactggaccg
     5341 gttgctcgtt cagttcgacg agttcgtcgc cgtgcgcgat gccaacgtca ggatcgagcc
     5401 aggcgaattc ttcagctttc tcggtccgtc gggctgcggc aagaccacca tcctgcgcac
     5461 catatcgggc tttctggagc caacctccgg tgccgtccgg attgacggca aggacatggc
     5521 gttcattggc ccgaacaagc ggccgacggc gctcatcttc cagaatctgg cactcttccc
     5581 gctgatgacc gtctgggaga acatcgcctt cgcgctggag atgaagggtg tcggcgcagc
     5641 cgatcgacgc aagcacgccg acgagctcct ggcgctgatc gcgctcgacg gccagggcga
     5701 caaatatgtg cacgagctct ccggcgggca gaagcaacgc gtggcgatcg cccgtgccct
     5761 ctgcgccgag ccctcggtgc tcctgctcga cgagccgctc tccgcactcg atctgaagct
     5821 gcgccagcac atgcgcaccg agcttcggac catccagcgg cgggtcggcc tcaccttcat
     5881 ctacatcacc cacgatcagg gtgaggcgct caccatgtcc gaccatgtgg cggtgatgcg
     5941 cgaaggcgtg atcgagcaga tcggcgacgg ccggacgatc tatgacgacc cggcgacggc
     6001 attcgtcgcc tccttcgtcg gcgagaacaa cgctctgccc ggccgggtcg cagagatccg
     6061 ggacggcgtg gcccttgtcg atacgtccgt gggtcgcctg cgcgcccgga tcgccacggc
     6121 tgcgcgtgac cggctgcgca ccgatgagga ggccatcgtc ttcatccgcc cggaggcact
     6181 cgacctcgac atcgctggag cctcggccga gactaccatc caggccaccg tcatcggcga
     6241 ggagttcgaa ggccccaaca tgcatctgca gctccggcac gacggcggcg cggagctgcg
     6301 cgtctcgctc gtcaacaagg gcaggacagg cacagccggc atcgggtcca gcctcgccgc
     6361 cgctttcgac ccgatgaaag cggtagccct gccctccggc gccctttcgg ccgaggagta
     6421 gcgccggcat ggcgaaacgc tcctggctcg gagggtttct cgatcgcaac gggcaggcgc
     6481 tcggcctcta catcctcctc gcggtcagct tctggctgct ctttttgatc atcctgccgc
     6541 agctcgccat gatcgacttc tcgctgcgcc acgacctgcc ggcggcccag cagggcgggc
     6601 ctcaggatgt ctggacgttc gagaactatc gccacttcat ttttggcctg ccgggcaacc
     6661 cggacggctg gaaccggctc gatgtcggtg tcttcggccg caccatcgtg gccgccgcct
     6721 tcgtcacgat cctcaatctc gcgatctgct atcccgtcgc ctactacctc gcccaggtcg
     6781 ccaggggacg gcaggcacgg gtgatggcgg tagcactggt cgtgccctac tgggtcaacg
     6841 agatcctccg ggccttcgct tttaggatca tcttcggcgc caccggcgtc atcaactcga
     6901 cactcctgac gctcggcatc atcgacctgc cgatcgactt catccgcgcc gatatcgccc
     6961 tctatgccgg cctcggctac gcctacgtcc tcctgatggt ctttccgctc tacaacgcca
     7021 tcgagagcct cgaccgcaac cagatcgaag ccgcccgcga cctgggggca ccctggtggc
     7081 ggatccacac ccgcatcgtc ctgcccttcg ccaagcccgg catcacgtcc ggctgcacca
     7141 tggtcttcat gctggcggcc ggtgccctgg cggcaccgca gatcctgggc ggaccctcca
     7201 atctctggtt cacccagctc atctacggcc agttcttcga gagcgtgaac tggccgcgcg
     7261 gtgctgccta cgccatcatc ctgctcgtcg cctgcacgct gctcgtgctc ctcgtgatgc
     7321 gcctattcaa ggtgcgcctc ggcgagatcg gcaagtgacc ggccgccggt caccccgctg
     7381 gtcgcaatgg gtcatcggcg cctacctcct gctcttcttc gcctacctct tcggcccgct
     7441 cctgatcatg gcggtcaccg ccttcaacag ctcgtcgttc ccgcgtgtca gtccctggga
     7501 atgcctgacc ttcgagtggt tcgtccgcct ggtcgaagac cagcggctga tgcaggggct
     7561 caggaacagc ctgatcatcg ccttcggcgt ggtggtgctc tcggtcgcca tcggcctcgc
     7621 cggcgccctc tttctcaccc agatcgcggc ccggagccgt gccggcacct acaccgtctt
     7681 catctcgccg atcctgatgc cgggggtcgt gctcggcatc gccacggtgc tcttctggga
     7741 ccgcgtcgcc ggctcgatcg gcatggccta tggcggtctc ttctatgacg gcatcttcct
     7801 caccgtcctc ggccagtcct gcttcatcgc ctcctattgc ctgctcgtct tcctcgcccg
     7861 tctgcagcgc ttcgacagcc agctcatcga tgcggcactc gacctcggcg ccaccaacac
     7921 ccaggccttc cgccgcatcc tcctgccgtt cctcaagccg gcgatcggcg gggctgcggt
     7981 catcgccttt ctcgccagct tcgagaacta caacaccacc gtcttcacca tctccttcta
     8041 caataccttc acggtcgagg tggcgcagaa ggtccggctc ggcatcgacc ctacgatcag
     8101 cgccctcgcg gtcattgtca tcgccatcag cctggtcgcg gccctcgcca acgaggcggg
     8161 gcagagccac cagcagcgcc ggctcgaagg ccacgggcgc ggcttcgtcg cgttcttcac
     8221 gcgcaatccc gcggcagtcc tggccgccct ggcggtcggc gtgatcgtcg tcgtcctcgc
     8281 cttcgccgtg cagcacgatc cggccgcctg caaggcccgc gcgctcgagc agcggctcga
     8341 acggcagcag cagtaccagc cggcgacgcc cgcgcccgcg gcacccacgc aatcggctcc
     8401 agcggcgccc ggagcccgcg acttgaaccc gggccagggc agcttcggcg acgtcttccg
     8461 tgggggcctc gccccgggct caacgccaac accggagcca acccccgcgc cgacgcccga
     8521 tacccgctcg ctcaatcccg gtcagggaat gttcggcgac gttttcgggg gcggccttgc
     8581 tcccggcacg acgcccgctc ccgaaagcca tgagggcgac agcgaggagt gaacggccgg
     8641 acgttcaggc tctgacccgc gcattcgtcg ggtcgtatgg aggatcaaga aagacttcgg
     8701 ccgacacgcg ctcgcccgcc acctccagct cgtacgtgcc atcgagcaca taggcccggt
     8761 ccacgcccgc ctcgcggcgc acatagccga ggccgaggtt cttttcgatc gtgtagcccc
     8821 agccggcgga gctcagccag ccggcgcgtt cgccgttccg gtagatggtc tcacgaccga
     8881 gcagcaccac agccgggtcg gcaacctcga agccggcaag gaggcgcggc agattctgct
     8941 gccgctgggc gagcagcgct tcgcggccga ggaacgggat ctccttcctc agcttcacgg
     9001 cccagccgag gccggccatc aggggcgtgt ggtccgggcc gatgtcgctg ccccaggcgc
     9061 gataggcctt ctcgagccgg agcgattcga tcgcccggta gccggcgagg atgaggccat
     9121 gtggctggcc cgcctcgcgc agcgcctcga agagagtcag cgcgaaatcg accgggacat
     9181 gcagctccca acccagctcg ccgacatagg tgacacggag tgcccgaacc gggcagccgg
     9241 caatgccgat ctcccgcacc gcgccgaacg caaaggccgc attcccgaga tcggcccggg
     9301 tcaggccctg caatacttcg cgcgccttcg ggccgaagag cgagagcacc gcatgggcgc
     9361 cggtgacgtc ggtcagccgc acatccatgc cggccgggat atggcgaagg atccagtccc
     9421 ggtcatgggt ggcgaagccc gtgccggtga cgatgtagaa ccggtccgac tcgaggcgtg
     9481 ccacggtgag atcgcactcg atcccgcccc gctcgttgag catctgcgta taggtgaggc
     9541 tacccaccgg cttcgccacg tcgttggagc agagccagga aagggcggcc tcggcatccg
     9601 ggccggcgag ctcgaacttg gcgaaggagc tctggtcgaa gagtgctgcg gcctcgcggg
     9661 ccgcccggtg ctcgcggcca acggcggcga accagttctg ccggccgaag ccgtagcggt
     9721 cctgtgctac ctcgcccggc ccggcgaacc agttggcccg ctcccacccc agcttctcgc
     9781 cgaagacagc gccggcaccc tcgaggcgat catagagcgg cgagcgacgg agcggccggc
     9841 cactggacat ctcctcctgc ggccaggcca tggtgtagtg cttggcatag gcctcgagcg
     9901 tgcgggtgcg aacccagtcg gtgtctcgat gcgccgctcc gaagcggcgg atgtcgaccg
     9961 cccagagatc gaagggtggc gcactcctgg cgacccattc ggccagggcc atccccgccc
    10021 cgccgcccgc cgcgatgccg aaggcgttga agccggcgcc gacgaagacg ttccggacct
    10081 ccggcgcctc gcccaggata aaattgccgt cggccgtgaa gctctccggc ccgttgaaca
    10141 tcgtctttat gccggcctcg gccagggccg gcacccggcc gagcgcgagc tccatgatcg
    10201 gctcgaaatg ctcccagtcc tcgtcgagga attgatgcgc gaagcgctcg ggcacgccgc
    10261 ccaaagccca gggaatcggg ttcggctcgt agccgcccat caccagcccg ccgacctcct
    10321 ccttgtagta ggtcagccgg tccggatcgc gcagcgtcgg caggtccggc gtgacgccgg
    10381 cgatcggctc ggtgatcacg tattgatgct gcaccgacac gagcggcacg ttgacaccga
    10441 gccgcgccgc gagctggcgc gtccactggc cgcaagcgag gacgagcttg ccgcaggcga
    10501 ccctgccgtg gtcggtctcg accgcgcgaa tacacccctg ctcgacgacg aagcccgtga
    10561 ccgcgacatc ctcgacgatc cgcacgcctt tggtcctggc acccttggcc agcgcttggg
    10621 cgatgtcgga tggattggcc tggccgtcgg tcggcaggaa ggcggcgccc accacgtcgt
    10681 cgacctgcat caacggccac agcgcctgcg cctccttcgc cgagagcaga tgcatctcga
    10741 gaccgaagct gtgcgccgtg gttgcctgcc gtctcacctc cgtccagcgg tcggcgttgc
    10801 aggcgaggcg caacccgcca ttgcgctgcc agccggtggc cagtcctgtt tccgcctcga
    10861 gccggtcgta gagctcgacc gagtagccga gcaatcgggt gatattggca ttggtgcgca
    10921 gctgaccgac caagcctgcc gcgtgccagg tcgagcccga ggtcagcttg tcccgctcga
    10981 gcagcagcac ctcgccaaca cccatggcgg ccagatgata ggccgtggag cagccgacga
    11041 tgccgccgcc cacaacgacg acagcagcag acgaaggcag atcggtcatg gccgctccat
    11101 ggcgtcgaag gcttcgagcg agcgctcgaa acgggcgagg ttctcttctg tatacgcttt
    11161 gaagtcgaat tcgatcgccg agtgcagctc ggagaccatg ctccacatcg cctcgcgcag
    11221 aagggaggct gtgagcatgg cccggctctg ccgggcgagc tcgtcgtcga ttggtcgatc
    11281 gaaataggcc tcgagcagcc aaaggcggtc ggcttcggcg agctcgctgt tggaggcgag
    11341 gccgcccaga tcgaagagcg gggagttgaa gccggcatag tcccagtcga cgagccaaat
    11401 ccgcttgccg tcgtcgatga aattggcggc gaggagatcg ttgtggccga gcacgagatc
    11461 gatccggccc acggcccgct ccagccgtgt tgcggtcgcg agccagccgt ccagcagcgg
    11521 cgccagccgg tggccgccgt cccggatcgt atgggcgtag tcgcgaatga catggaagac
    11581 ccaaaacgcc agcacgggcc cgcgcaaatg gtccggcatc tcgcaatgga cccgccgcag
    11641 cagcggcagg atccgcgtct gcatggcacg ctcgcggacg tcggcgggcg tcagcgtgcg
    11701 accttcgaca aaggcgagga cgagcacgcc cggctcggcg tggataacgg cgggggagat
    11761 gccggctgcc gccgcggcct cgctggcagc ccgctcgttg aagcgcagca cgccgtgcag
    11821 ggggatgtcg ccgccaaccc ggacgacata gcggcggccg ccatcctgga cggtaaagtt
    11881 ggtgttggtg atgccgccgg cgagcgggcg aggctccacc ggccccttcc agatcggcag
    11941 ggcggcggcg cgggctgttg cgtccccggt cacgcttcga gccccaggcg gtggcgcatg
    12001 cgcaggctgg cggcggcgat caggcgatcg gcaatgatgg cgatggcggc aacgcagagc
    12061 ccggcgacga ggcccaggcc cacatcggcc ttggtgagcg cgatatagac gtcctggccg
    12121 agatcgtggg tgccgacgag tgccgtgatg acgagcatgg aaagggccag catgatgatc
    12181 tggttcaggc cgagcaggat tcccgggact gccagcggca ggcgaatgcg ggtcaggatc
    12241 tggcgctccg tgcagccggc tgccgttccc gcctcgacca gctcggcagg tgttcgggct
    12301 acactgtggc gggcatagcg aatggccggt gccagcgcat agagcacgat ggcgatcatg
    12361 gcggcgaaat cgccgacgcg gaagagcatc acgacaggga tcaggtagac gaagcgatga
    12421 gcgccagcca ggagacgggc gcggtccagg ccaccgcggc cggtccggcg tcagtctcag
    12481 tcaggcgatg gtccgggtga gatcgcgaaa gcccaaaggc gcgagtgctg cgtcctcgac
    12541 gtgccagctc atgaacgccc ggagccagtg ggcaaaagga aactgcagat cgcgcggcag
    12601 ggcatcgctc ggcaggaggg cgaagccccg ggagatggca aatacgatcg cgagaggttc
    12661 ggcctcgtcg cagggccgga gggtccgcag tcacggcggc catacctgac gtaggacgcc
    12721 catgacgatg tcacggtcga cggcaccaag caggcgaccg acctcgatga cggcgagggg
    12781 tccggatgcc gcctcgacct tctgagctct cgagctcggc gacgagcggc gcgtccgcgc
    12841 tggcgtggat cacctcgtag tctaggccca gcgcctcgat tcgctcctcg tcgaagccgg
    12901 tccggtcgtg gggcatcggg cggatcggct agtccgactc gacggcgcca taggagctcg
    12961 ccgcgagcgt caggcccaga accatggcgc agccggtgag gcgcctcttt gtcatgttcc
    13021 ttcccgtgcc cagtctcatt gcgtcgcccg atcattcgtt ggccccgacg cagggtcaac
    13081 cactgtcacg ccgctcgaac gtcgtgacgc tagccgctat cggcactaga acggctcgat
    13141 gcagggagat aagagcatga ccaaggcgac accgcgcatc gccttcatcg ggctcggcca
    13201 gatgggctcg cgcatggccg ccaggctcgc cgaggcgggc ctgccactcg ccgtccacga
    13261 cgccgatgca ggtgccatgg agcctttcgg cgccgagcgc agccatccct cgccaaaggc
    13321 tgccgcggcg ggctgcgatg tgctcctgac catgctgccc aacgggcggg tggcacagga
    13381 ggcggtgctg gccgccgacc cggccccggg cgtgctggtg gtcgacatgt cgtcgagcga
    13441 tccggccgac acacaggcca tgggcgcagc gctagccgac ctgggggtcg cgctcgtcga
    13501 cgcgccagtc tcgggcggca tcaagcgggc ggccacgggc gagctcgcaa tcatggcggg
    13561 cggggaagcc cgggacctcg agcgtgctcg cccggtgctc gagcatctcg ggcggcggat
    13621 cttccactgc ggcccgctcg gcgccggcgg ggccatgaag gcgctgaaca acctgctctc
    13681 ggccgcgggc ttcgccgcca ccatcgaggt gctgcagatc ggccgacgct tcggcctcga
    13741 tccggagctg atgctcgacg tgctgaacaa ttccaccggg cgcaacaaca cgaccgaggt
    13801 caagatcacg cccttcgtgc tctccggtcg attcgattcc ggcttcgcgc tcgatctcat
    13861 ggtcaaggac ctgacgacgg cgctggcact cgcgggccgc accgaaaccg atgtgccgct
    13921 gggccggctg gccctcgacc tctggcggga ggccggcggc gacctcgaag ccggcgtcga
    13981 tcacaccgcc ctcgcacgct ggtacgagaa gcgaaccggc acacagctcc gctcgtgagt
    14041 tgcaggcccg cgatcctgcg gtcagtcgct atccggccac agcctgagcg ctcgcgcctt
    14101 ctcggcccgc cagctctcga gatcggacga agccggtccc aagccatccc tgccggccga
    14161 ggccagcacc gcgccgatcc ggtccgccac gatctccgcc tcgccctcgc cgagcgagcg
    14221 ggggtccatg acgagccagc catgctcgac ctggtgcgaa cgaacggcga tcggcgggtc
    14281 gccccgctcg agctcccgga cgagctgcag ggcgttgaga ccggcctcga tcgggtcgac
    14341 ctcgagcttc agccggtcga acgggttgcc ggtcggatct gggtcgatgg tcgcctcgat
    14401 gccgggcaag gcgcccaggc gccgccgcca gtcctcgagc acctcgcgca tgccctgccg
    14461 ccgggcggca tggtcgcgcg tgccccatgc ctcgagtgcc gccaaggcac cggcaacgct
    14521 ctccttgccg gccttcattg gccgggcaat gccgaaattc tgaagatagg cggcgcggac
    14581 caggggcttg cgtccggcga cgatgccggc ggtcgcaccg cccaggaact tgtggctgct
    14641 gtggatggtg agatcggcgc cgtgagccag atagccgcgg agatcgtatt cggccgcgag
    14701 gtcgacgatc accggcacac ctttgacgtg gcagatctcc acgaacgtgg cgaaggggat
    14761 ttgcccctcg ccggagcagt ggtggctgac gacgaaaagc cctgctgccg tctgcggtcc
    14821 gatcgccgcc gcgagctgat ggctgcgcgc ggcgttgacg tcgccgatca cgactgggcg
    14881 cgcgccggga aggcggatgt cctgggcgat ggaatgccca taattgacga catggccggc
    14941 ctgaatgacc acttcgctgc gcatgccggt cgtgtcggga agccgctcga tgcgggcgag
    15001 atcatcgccc gtcatcgccc cggccacgcc catgcagatg ccggccgagg agcaggcggc
    15061 gacgaagccg gcctcggctc ccgtggcgcg tgcgatgatc cggctggccg cagcctgcaa
    15121 ctcgaggacg tcgacgccgc gcgacatgat gttggccgcg gcctcgatcg cctccgggca
    15181 ggcgctgatc ccgccatggg tggtgagcgt gccggtcagg ttgatgagcg gccgcacgcc
    15241 gaggcgtcgc cgcacgtcgg ggagcggttc ggtcatgccg ggtacacctc cacctccggc
    15301 tcgaacacca cgtcgggatc gagcggaaac atcggccgcg cgcagacttt atggccgaga
    15361 agcggaagat tggcggaggt ggcgcccggc acatcgacca cgaagttctc ttcggcgaag
    15421 gtgaagaagc gtgaataggc gcccggcgac ttgatgacga tgagatcgaa gtcccgcgga
    15481 tcgtggccat gcgcgaggaa gacaccgcgg tccatcaggt gcaccggccg gctgatgacg
    15541 atgagcgtga tgttgtctgc ctccagcacc gccgtgcgac cggcattggc catgaggccg
    15601 ttcacctcgt ggcgccagcg accctcgccg agcattgcga cccggacctc gaccggcaac
    15661 ggcggaaagc gctccgggtc gagcgagcca ccgatggcaa gttgcaggcg cgcaccgact
    15721 ccggcggcgt gggcacgctc caccgccggc gcatcgacga tgggcatgag aacccgcccc
    15781 ggatagccgc gctgcagcag gcccttgagg atggcattgc tgtcgccaga ggccccgctg
    15841 ctcggcgcat ccgcggcgtc ggtaaatgta acgggtgccg tcgccgtctt cgccgcctcg
    15901 atcgcgccgt cgaggctcac cagcggcgcc cgcatgcgct cgcgctgccg ccagaagccg
    15961 accgccatcg ccagcgcctc ccgctttgct gcatccacgt cgccatcggt gatgaccagg
    16021 gactgggagc agagctcggg cacgtcggtg aaggggttgc cgatcagcat cgccgcagcc
    16081 agggccacgt tcgcctccag cctcttcgca tttcgaatga tctcgccgta gcagccactc
    16141 ttcgtgaaga gctcgggtcc gcgcaccagg gccggaacct tgacgcgtgc catgaccggc
    16201 cgcgcacccg tatcgatgat gcgcagcagc aggcgcgccg cgcgggcgcc agtgtcgatg
    16261 aagtcctcgt gcgggtaggt gtgatagacg gcggtcgccg tcatgttgcg cagcatgcgc
    16321 gccgtcagga tgccgtgcag atcgagcgaa gttacgatcg ggacatccgg tccgaggatg
    16381 ccgcgggccg cttcgagcag atggccctcc ggatcgagct ccccctcggc acccatggca
    16441 ccgtgcagcg agaaatagag cccgtcggct ccctgcgcat gctcgcgcag ggcggcgagc
    16501 agatcgccct tgattcgccg aaatgcatcg gcacgcagca ccccgccgct cggcgcctta
    16561 gctccataga caggcaccac cgtgacatca gagcgcgccc cgagcacgtc gagggcaccg
    16621 cgcacacacg tgtcggcctg cctgttggcc tcgatcaggg cctcgcccat ggcgatactg
    16681 aacgactcgt actcggtcgg caccgggttg aaggacgaga tttcctgctc gcactggagc
    16741 acaaggattc gtcgcaacgc tttctccccc ggaagcagca aggactggct ctcacggtct
    16801 ttccaaggcg atcccgcgcg ggcaggtatg ggccatacac acgcgaggtc atcatgtcat
    16861 cgaaggtgtt cgcgtccgct gtcatcgggg cactgctgct cggcacgtcg cccgcggtca
    16921 tggccgccga tctcgccatc tggtggacca agggtgtcac cgaggacgag tagaatgcgc
    16981 tcaaggcggt cgtgacgaag tagcagaccg gcaagacggc gggtctcagc ttctacggat
    17041 cgggcgacac cgaggccaag acgatcgccg ccctggaagc cgggtcgccg ccggacctca
    17101 tcttcgacca cgcttacgac ctcgcgttca gccccacctg ggctacgacg gtcgtctggc
    17161 cgacgtcagc gtcgtgctgg agccgattgc cgaccagttc cagccatcgg ccctgagatc
    17221 cgtgtacatg ctcaatggcg aaaccggcga gcgcagctac tacgccatcc cgtggacgca
    17281 gatgacgccg cacgtccact actggaagga cctcctggtc cagcccggat tcaccgagga
    17341 cgacatcccg aacggatggc agcctttctt cgacttctgg tgcgacgagg tgcagcccgc
    17401 gctgcgggcg aagggcgagc gggtctacgg tctggggctc ggcaccggca gcggcggcaa
    17461 cgatcccttc ttcaacatcc acatcttcct caatgtcttt ggcgccgagg tcctcgacgc
    17521 ggacggcaac ctgcagatcg acgacccgga cgtgcgggcg cgggtgatcg aggcggtcaa
    17581 gtcgttcgtg aagccgtcgg ctgagcagtg cgtgccgcca gatggggtga actgtgccgg
    17641 cgccgatgac attgtctact tcatcaaccg caagaacctc gtcgccttca acccgacgct
    17701 ctcgatcccg gcaacgcagc tcaaggacaa ccccgagaac tactacgaga acatgcgcac
    17761 ccgcccctgg ccgaagggcg tcgagagcga gccggcgccg gccatggttt cggtcaagca
    17821 ggtgctgatc ttcgaggtgt cgccgaacaa ggagaacgcc aaggact
//
LOCUS       DAOTFI010000095        17794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3817_length_17794_cov_7.519702, whole genome shotgun sequence.
ACCESSION   DAOTFI010000095 DAOTFI010000000
VERSION     DAOTFI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17794
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3817_length_17794_cov_7.519702"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..616
                     /locus_tag="P5558_11285"
     CDS             458..616
                     /locus_tag="P5558_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HRY24938.1"
                     /translation="MDLLRILLAILLPPLGVFLQVGIGAQFWINILLTLLGYIPGIVH
                     AVWIIARR"
     gene            613..1008
                     /locus_tag="P5558_11290"
     CDS             613..1008
                     /locus_tag="P5558_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HRY24939.1"
                     /translation="MSTRALGEATKVVDRSADLRRLKTVAYWLDDRFRIPVLGWRVGL
                     DGLVGLVPGIGDAATTVAALWILREAHRLGVPRHKLGRMAINIGVDTVLGAVPLIGDA
                     FDLANKANRRNLRLVLDHLEPERHTGTRR"
     gene            1052..1348
                     /locus_tag="P5558_11295"
     CDS             1052..1348
                     /locus_tag="P5558_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HRY24940.1"
                     /translation="MPHSPTCRDVLVSRKLAGREPAGRLELHFRPHECLEHVTDPLPR
                     NVSAVAIMGRTMFAAGDETATIERLARTGDGDGRRAALAVLADLDRDPNRMPPI"
     gene            complement(1357..1689)
                     /locus_tag="P5558_11300"
     CDS             complement(1357..1689)
                     /locus_tag="P5558_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24941.1"
                     /translation="MPARAADDAKDDDRGSSTLAEDLERLREDIAGLKSTLAGLGKRG
                     ATEARAAADAKLDELHDELERLAGDLHLRGEDALFGIEQRVRDHPVTSLLAAFGIGLI
                     LSRLLDRR"
     gene            complement(1759..1998)
                     /locus_tag="P5558_11305"
     CDS             complement(1759..1998)
                     /locus_tag="P5558_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24942.1"
                     /translation="MPDKAQHLLEQVTMHLAVLELRLAADPEFRCLCADHGEALEALG
                     RWEASTDPQRTSRIEEFRRLVAELEQEIMAELGVT"
     gene            2226..4052
                     /locus_tag="P5558_11310"
     CDS             2226..4052
                     /locus_tag="P5558_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24943.1"
                     /translation="MREQANSAQPGEQGDGEAASGVKAAAVRAQLARILASPSFDASE
                     RNRRFLEHVVEETLAGRGERIKAYGVALEVFARDDSFDAQSDPIVRIEASRLRRSLER
                     YYLLAGQDDPVRIEIPKGGYMAAFKTTDADAAHTAAPAPSAEAPAAAAQRPQTERTLT
                     VLAFCLVLIFAGLAGGWALATYWPGRTAAGSGAAGRGPVLLAVPFEDDGSQVQGDVRQ
                     SATLVHGFSREVVAGLARFGGLSVFDAETAGRSLGEADMQRLAALLDADFLLTGGVSV
                     SDDRFAVTASLIDSASGRHIWSGHFDGDVTAASIIAVRDQLAGRVVQELAQPYGVLFG
                     EQLREQEGKPPEALTSYECVLRFHRYWRELDRDGYSPVRQCLERTIVADPDYAQALAA
                     LALLLADGYRLGYDPDGAGGGTLPRALALARRAVELAPADSQGYVALQLVYWLMNDVE
                     RSLAAGERGLALNPNDTELLAELGRRYCLRGMFDIGVPMIDEAFARNPILPGHYRIGP
                     FLARYAAGDYPAALIEAKRIGIPDTVFGHIALAMAHAKLGNTSEAAAAVDRILAIDPA
                     YGDHVRLDLAKRALHPELIETVVDGLRLAGLAVAAMPSPSSY"
     gene            4170..4955
                     /locus_tag="P5558_11315"
     CDS             4170..4955
                     /locus_tag="P5558_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24944.1"
                     /translation="MVKRTPVTIVAALALATAWGVPAASAQEATEAVPADAGWSFALT
                     PYAWFIAMKGDVATFSGAPPINVDADFSDIIDQTNGAALLAGEARRDRFGLLFDINYL
                     DLSGKGDTPGALFGDAEMDNRTLFSDIAGFYEVGRDALFSLDVYAGARLWYVETEVNL
                     RAGLLPNRTDQDSEAWADPIVGVRGQALLGRGFSLQGAADYGGFHVGSKNTWQVIGTL
                     GYDFNDRLSARAGYRYLSVDYDDNGFVWDVDIHGPIIGLTWRF"
     gene            4987..7551
                     /locus_tag="P5558_11320"
     CDS             4987..7551
                     /locus_tag="P5558_11320"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRY24945.1"
                     /translation="MTGKHHLVLGLGLMLIAAGPAQAQQVTGTLGSPSATTTIPGNQL
                     PAPAPAFGGVIKDDALSSTPWWPPRIVPPEGAPNILLIMTDDAGFAVPSTFGGVIPTP
                     TMDRIAEAGLKYNRVFSTSLCSPTRAALITGRNHHSVGFGVIAEQATGFPGYDSVIGV
                     DNATIGRILRDNGYATSWFGKNHNTPSYQASQVGPFDQWPTGMGFDYFYGFVGGDANQ
                     WGPNLFRNTTQIYPFDEHPGTLEMDRSDAAIWPVTGEESSWNLITAMADDAIDWMTRI
                     HQTDPSQPIFLHYVPGANHAPHHPTKEWVDKIHAMHLFDDGYEKLRERIFANQQKLGV
                     IPQDLELTPWPDDVLTPWDKLSPEAQKLYIRQVEVFAAYVAYNDHEIGRVVQAFEDLG
                     KLDNTIVIYQNGDNGTSAEGGPEGTFSEVAFFNGVRPPVDVQMKFYDAWGTELAYNHM
                     SAGWSWAFDTPFDWFKQNASRLGGTNQNMVISWPDRIKDKGGLREQFMHVIDYVPTLL
                     ELTGIEAPKLVDGIEQKPIEGTSFAYTLDAANAEAPSRHTRQYFEMMGQWALYDNGWL
                     LSTKVNRTPWDAFSPANRDPLNNQVFQLYNLEESWNQADDIAAAHPDKVEAMRAMFLE
                     EAKKYQVLPLDASVGARVAAQRPSLTAGRTELVYTRPMTGLPQGDAPYLLNTSYTITA
                     DITVPEGGAEGMIVTSGGRFAGFGFYLLEGKPVFLWNLLDLERQKWEGQDALEPGQHT
                     IEFDFVYDGLGVGTMAFNNFTGIGRPGTGTLKVDGKVVATQKMEKTIPIILQWDESFD
                     IGSDTITGVNDADYRPPFPLTAELDKLTIRIDRPQLSPKDIKELEEAMKKVDAARE"
     gene            7616..8500
                     /locus_tag="P5558_11325"
     CDS             7616..8500
                     /locus_tag="P5558_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24946.1"
                     /translation="MREDGLVISTSLPRCLALVLAVQLLTMAAARAEEPAAPPAAPPS
                     TLDLAKKAQNPVADLISVPLQSNFNFGYGAKDAPESSSTQYLLNIQPVIPISLGQSGF
                     NLITRPIVPVIRQPDLIEGGETWGVADIQIQSYLSPAGSKELIWGVGGVLQPPTATDG
                     DKLGTQKWSAGPAAVVLSMPGKWVFGALATQLWSFAGKSDREDVSQTLVQPFINYNFD
                     HGWYVSSAPIMTANWKADGNDNTFTVPMGGGFGRLIRVGKLPVNLQAQAFYNVVKPDD
                     DPTADWTLRLQMQFLFPK"
     gene            8510..10096
                     /locus_tag="P5558_11330"
     CDS             8510..10096
                     /locus_tag="P5558_11330"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HRY24947.1"
                     /translation="MDKSIHSPAATPVRAGRCVTSAIVGAMAMTGMLALQPFTVRAQA
                     LDGPPAMPAFEFSTAMPSGVASPDEVESRLGTLKFFDGFPDKATAEVVYDNLDFQRAV
                     QAYLLALPAVNQAGNLNAIASLGPLNATVPIWEELVDSRTIELTANDNTPYTWFWIDL
                     TDGPLVVEVPPEVLGLVDDMWYRWVIDVGITGPDKGKGGKYLFLPPGFAGDVPEGDFV
                     AVVHATTYRLWIPWRSFLENGDPKPGVERVKAHTRIHAPGSEPVAPTFVDMSGKPFNM
                     VNPADYAFWELLNDVVQKEPAGSLDEVTLGFFAAIGIEKGRPFAPDDRMKKILTEAAT
                     VGDATARTIAFHMRDRNAYYYADSNWQLPFVGGYRFQSSPGVLNLDGSAFFFFMATGV
                     TPAMEEKMVGRGSQYAWTARDAEGEPLDGGKSYRLHLPKDIPVKDFWSVILYSDQTRS
                     MIQTDQRFPSVSSQTQGLLVNADGSVDVWFGPEAPSGKEKNWVQTVPGQAWNTILRLY
                     GPLEPWFDGSWKPGEIEPVG"
     gene            10180..10905
                     /locus_tag="P5558_11335"
     CDS             10180..10905
                     /locus_tag="P5558_11335"
                     /inference="COORDINATES: protein motif:HMM:NF025724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HRY24948.1"
                     /translation="MPNTTTSAWRLGAIGAVALLAACTQSSSQIETTAALPKPQVIVV
                     QDFAVQAGEVQLDPGLSGTIDETIKASTLPPNTAEEAQVGRQVADALAQKLVTEINDL
                     GLNAQRGNTLPDGMSSGLEITGQFVGVDQGNRTERVAIGLGAGRSDVRVRAQVFEVTP
                     SSRTLVDEIEVDAKSGLQPGMAEMMGVGALAGHFVASTVVSGGLQVADESLGTSVVAD
                     SDRAAKGIAKQLATFFAKQGWTN"
     gene            10902..11216
                     /locus_tag="P5558_11340"
     CDS             10902..11216
                     /locus_tag="P5558_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HRY24949.1"
                     /translation="MSFGTKFERFRAFPASSSRRKGETMARMIGLSLAAAIGLTAAMA
                     SAQEGMKIARACGDDIDRLCAGVPPGEGRIKACMKAHMTELSAPCMDTLLAAIAAEKE
                     PE"
     gene            11342..12112
                     /locus_tag="P5558_11345"
     CDS             11342..12112
                     /locus_tag="P5558_11345"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HRY24950.1"
                     /translation="MLLLAAASFLLTHVAVAQEAQSRPQREFALPVTPELEPGAMELL
                     QAMGERLAAARTLSFTAVATYESPARTGLPLAYTTLSEVTLQRPDHLRVVTPGDGPAI
                     EFYYDGATMMAYQPDAGLSAIADAPPTIDAMLQTLFGLSATYFPFTDVIVADPYGDLA
                     TGLQLAFVVGQSTVVGDTLTDIVVIADPTLQMQLWIGAEDRLPRRIQASFFDELGHFR
                     HVLELSDWRLDPELPPDTFSSAAAAAATPIPFAPPGTP"
     gene            12129..13007
                     /locus_tag="P5558_11350"
     CDS             12129..13007
                     /locus_tag="P5558_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24951.1"
                     /translation="MMRLAILALVGAAAIVLPREAGAWAHAGRFGVASGGGGSWHADG
                     WRGGSASGGDGSWHGQAWRGGSASGGDGSWHATGAAGGTGSGGDGSWHANSAWGGSAS
                     GGGGSWSAHGAEGGSASGGDGSWHATGAEGTKVQGSYAHATSVDVAYPHAYRGPYYAT
                     YHPPVTVDHYSSTCDNCGGWSGGAVAAAGALGLAAGAAIDASAAKADTAAATSSAYAA
                     GVSAGEAAAPASAATSPAQPTKTAAAPSGTVYTELPANCAYKVVKGSNYFDCGDMWLT
                     AAYGANGLHYTVVPAP"
     gene            13036..13503
                     /locus_tag="P5558_11355"
     CDS             13036..13503
                     /locus_tag="P5558_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24952.1"
                     /translation="MEMTAEESLWVADWLWSLPLIAFCVAIHVVGLALINDRVVGFLR
                     DAIDNRNFLRLFAVVIGAVALLATTLHGLEAWLWALAYWLLGALPDFRSAMLYSISAM
                     TTYGHSDISLEAEWRMMGALESLCGMLLFGLTTAFLFAAIQKVWPLGARDRRL"
     gene            13642..14424
                     /locus_tag="P5558_11360"
     CDS             13642..14424
                     /locus_tag="P5558_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24953.1"
                     /translation="MTRLSTMAVAAGIALASGAQAQDISTELPPEDRWTVTVAPYLWM
                     LSLDGNTTVGGIKADVDVPFKDSIQDLSFGGMLLTTVRKGKLGFSINGVYTRVSPDNS
                     VGPLDIDSTADLAQLAGGPFYRMVEWQYGETSAGQPLRFYLEPYVGARLNYLRVEVEV
                     RGGQQQDESQTWVDPIVGSRVGLDLTDRLALFGGADVGGVVTGSDFAWNAQAYLGYQT
                     SVFGRETFLSFGFRAYHADYEGGNFKWDATQYGPIIGTAIKF"
     gene            complement(14467..15990)
                     /locus_tag="P5558_11365"
     CDS             complement(14467..15990)
                     /locus_tag="P5558_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY24954.1"
                     /translation="MDVLGSLGDGFWIAITWQNMALALLGCFLGTVMGALPGLGPSNG
                     VAILIPLAFTLGLPATPAMILLTSVYFGAMYGGRISSILLNIPGDEPAMMTCLDGYPM
                     AQQGRAGEALALSGVASFVGAFLSTWGLVFLAPQLVKLALLFGPAEYFALFTLAFATL
                     GGISSSNQAKSAFAAALGVGIAMVGVDGQTGVPRFTFGEVHLYDGIDFLVAIVGLFAL
                     SEVFVFLEHRGKETAEGKAATVGIGRITPSMSVLRKCTPTMLRGSALGFVAGVLPGAG
                     ASLGSFIAYSFEKKLVDREGTFGKGDPRGVAAPEAGNNAAAGGALVPMLALGVPGSGT
                     TAVLLAMLLALNITPGPLLFQQNPDVVWGLIAALFIANFMLLAMNIPMVGMFVRVLLV
                     PPRILMPIVAMVSFVGIYGISGSSFDLLVMVVFGLLGWVLRKLDVPLVPIILGVLLGN
                     QMEANLRRALTISDGEWGILFASPLALVLWAIAIIGFILPIVVGGVMRRKMRGGLAD"
     gene            complement(16000..16431)
                     /locus_tag="P5558_11370"
     CDS             complement(16000..16431)
                     /locus_tag="P5558_11370"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY24955.1"
                     /translation="MSDRVFGGIGLALAVFFIWQATQIQLSFISDPVGPRTFPIIVGV
                     VLGLASLVILIRPDAAPDWPRATRLLEIAVAAAVMVAYALLLPELGFVIATALAATYL
                     TWRLGTVPLWSFVVGIAISLGIYAVFALALGLSLARGPLGF"
     gene            complement(16514..17521)
                     /locus_tag="P5558_11375"
     CDS             complement(16514..17521)
                     /locus_tag="P5558_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRY24956.1"
                     /translation="MRFSLDSRGLFAAGALAGAMLAGAMPADRALAFEPENPECIAPA
                     NPGGGWDFTCRQVGKTLQDLGLVDGTVQVTNIAGGGGGVAFAEVVNKRADDNDLIVAA
                     SSATSTRLAQGAYPGNTMDQVRWLASVGADYGIIAVAKDSPIATLPELMDQIKADPKS
                     VSIAGGSAVGGWDHLKVLIAAQAAGIDDVRTIKYVAFDGGGEAVTQLLAGSVQAFTGD
                     ASEAKGFVDSGDIKVIAVLAPERLEGDFASFPTAAEQGLDVVGANWRGFYAPGNMSDE
                     AYDFWVESIGSVYDSDEWKSIMAQNGLAPLDLRGPEFEAFVAESVSRINGLSKEIGLI
                     Q"
BASE COUNT         2912 a         6027 c         6161 g         2694 t
ORIGIN      
        1 ccgtcgccca cgaggcgcat cggcagggcc tcgccccggg ccaaaagctc gacatcggtg
       61 atgtcgggcg cccagaagtc gaaacgggtg ccgcccggga tcggttttgg tccgaaacgc
      121 atcatgcggg attcccttcg ggcgcgggca ggtgacggcc gcggggtcga tcgccgcgcg
      181 tacggcccgc cgcgacgaac ggctcttcgg cactgccgtt cccatggatg tcgtgatcgt
      241 gcccggtctc gccattcgcg gcactgcggc tgtccttgta gccgatggtg tcgggcagcg
      301 tgaagccgcc atgcgtgcgc ctgcacgaac ccggcgcacc gaagcggcag cacctcgggc
      361 ggaacctcgg gcggccccgg ccattggctt ggcaccggca cgggcgatcg atgctccgag
      421 ccgagcgccc catttcagcc gcccattgag gaaacagatg gacctcctgc gaatcctgct
      481 cgcgatcctg ctgccgccgc tcggcgtctt cctgcaagtc ggcattggcg cacagttctg
      541 gatcaacatc ctgctgacgc tgctcggcta cattcctgga atcgtgcacg ccgtctggat
      601 catcgcgcgc cgatgagcac tcgggcactg ggcgaagcga ccaaggtcgt ggatcggtcg
      661 gccgatctgc gccggctgaa gaccgtcgcc tactggctgg acgaccggtt ccgcattccc
      721 gtcctcggct ggcgggtcgg gctcgacggc ctggtcgggc tcgtgccggg catcggcgac
      781 gccgcgacca ccgtggcggc gctctggatc ctcagggagg cgcatcgcct gggggtgcca
      841 cgccacaagc tcggccggat ggcgatcaat atcggcgtcg acaccgtgct gggcgccgtg
      901 ccgctgatcg gcgacgcctt cgatctggcg aacaaggcca accggcgcaa tctgcgcctc
      961 gtgctcgacc atctggagcc cgagcggcac accggcacgc gccgctgagc gccggtccgc
     1021 caacccgatt cccggcctgg aggctgctcg catgccacac tcgcccactt gcagggacgt
     1081 ccttgtcagc cgcaagctcg caggccgcga gcctgccggc cggctcgagc tgcattttcg
     1141 cccgcacgag tgcctggagc atgtcaccga tcccctgccc cgcaacgttt cggccgtggc
     1201 gatcatgggc cggacgatgt tcgccgccgg cgacgagacc gccaccatcg agcgcctggc
     1261 gcggaccggc gacggcgatg gcaggcgggc agcgctggcg gtcctggctg atctcgatcg
     1321 agaccccaat cgcatgccac caatctgaac gccggctcag cggcgatcga gcaggcggct
     1381 caggatcagg ccgatgccga aggcggcgag caggctggtg acggggtggt cgcgcacccg
     1441 ctgctcgatg ccgaagagcg cgtcctcgcc gcgcagatgc agatcgccgg ccagccgctc
     1501 gagctcgtcg tgcagctcgt cgagcttggc gtcggcggcg gcccgtgcct cggtggcacc
     1561 gcgcttgccg agaccggcga gcgtgctctt gaggccggcg atgtcctcgc gcaggcgctc
     1621 gagatcctcc gcgagggtgc tcgatccccg gtcgtcgtcc ttggcgtcat cggcggcacg
     1681 tgcgggcatg ggcgatctcc tcggctgtga gggtccgatc tgaccaccgg ccagcggcag
     1741 gcgcaaaggc tttctggctc aggtcacacc gagctcggcc atgatctcct gctctagctc
     1801 ggccacgagg cggcggaact cctcgatgcg gctggtacgc tgcgggtcgg tggatgcctc
     1861 ccagcgaccc agcgcctcca acgcctcgcc gtgatccgcg cacaggcagc gaaactcggg
     1921 atcggccgcc agccggagct cgagcacggc cagatgcatg gtcacctgct ccagcagatg
     1981 ctgcgccttg tcgggcacgg cccggtccac ccctctcgag cgcgatggtg gcggcgtccg
     2041 ccgaaagcgg gacaccgtat tgcgatgccg agcctcgggc acgcgggcgg ctccgggcag
     2101 tacgttgctg ttgcctacgt gcgccgggac agtgttcgcg gcggtaccgg atcggctgtg
     2161 gcggggcgac gatcgtgcta ggctgtccgt ggcgggtcag agtcgggcga cgtggcggga
     2221 gcgagatgcg cgagcaggcg aattcggcgc agccaggcga gcagggggac ggcgaggccg
     2281 catccggggt caaggcggcc gcggtgcggg cccagctcgc ccgcatcctg gccagcccgt
     2341 ccttcgacgc ctcggagcgc aaccggcgct ttctcgagca tgtcgtcgag gagaccctcg
     2401 ccggccgcgg cgagcggatc aaggcctatg gcgtggcgct cgaggtgttc gcccgcgacg
     2461 acagcttcga tgcccagtcc gaccctatcg tccgcatcga ggcgagccgg ctgcgacgca
     2521 gcctcgagcg ctactacctg ctggccggcc aggacgaccc ggtgcggatc gagattccca
     2581 agggcggcta catggcggcc ttcaagacca ctgatgccga tgccgcccac acggcggcgc
     2641 cggccccatc agccgaggcg ccggccgcgg cggcgcagcg gccgcagacg gaacgcaccc
     2701 tgacggtcct ggctttctgc ctcgtgctga tcttcgccgg gctggccggc ggctgggcgc
     2761 tcgccaccta ttggcctggc cggacggcgg cgggcagcgg tgctgcgggc cgtggcccgg
     2821 tccttctggc cgtgccgttc gaggacgacg gcagccaggt gcaaggcgac gtgcgacaga
     2881 gcgccacgct ggtgcacggc ttcagtcgcg aggtggtcgc cggcctcgcg cgcttcggcg
     2941 gcctctccgt gttcgacgcg gaaacggccg gacgttccct cggggaggcg gacatgcagc
     3001 gtctggcggc gctgctggat gccgacttcc ttctgaccgg cggggtgagc gtgtccgacg
     3061 accgcttcgc cgtgaccgcc tcactgatcg acagcgccag cgggcgccat atctggtcgg
     3121 ggcacttcga cggcgacgtc accgccgcct cgatcatcgc cgtgcgcgac cagttggcgg
     3181 gtcgtgtggt ccaggagctc gcccagccct atggcgtcct cttcggcgag cagctgcgcg
     3241 agcaggaggg caagccgccg gaagcgctca cttcttatga atgcgtgctg cgctttcacc
     3301 ggtactggcg cgagctggat cgggacggct attcgccggt ccgccaatgc ctcgagcgga
     3361 ccatcgtcgc cgatcccgat tatgcccagg ccctggcggc cttggccctg ctcctggcgg
     3421 acggctaccg cctcggctac gacccggacg gagcgggcgg cgggaccctg ccccgggcac
     3481 tggcgctcgc ccgccgcgcc gtcgagctgg cgccggccga ctcgcagggc tacgtggcgt
     3541 tgcagctcgt ctactggctg atgaacgatg tcgagcgcag cctcgcggcc ggcgagcgtg
     3601 gcctcgccct caaccccaac gacaccgagc tcttggcgga gctcggccgg cgctactgcc
     3661 tgcgcggcat gttcgacatc ggcgtgccga tgatcgacga ggccttcgcc cgcaacccga
     3721 tcctgccggg gcattaccgg atcggcccgt ttctcgcccg ctatgcggcg ggtgactacc
     3781 cggcggcgct gatcgaagcc aagcggatcg gcattccgga cacggttttc ggccacatcg
     3841 ccctggccat ggcccacgcc aagctcggca atacctcgga agccgcagcg gcggtggacc
     3901 ggatcctcgc catcgacccg gcctatggcg accatgtgcg cctggatctg gcgaagcgag
     3961 ctctccaccc cgagctgatc gagaccgtcg tcgacgggct gcgcctcgcg ggtctcgctg
     4021 tggcggcaat gccatcgcct tcgagctatt gaaacgccgt ccggacggcg ccttcgcagc
     4081 accggtagct tacagcaacg tacttcggcc ccggatcgat ccggggccaa tcgagatcct
     4141 ccgacgcgtg ccctgttcaa ggagcaagca tggtgaaacg aacgcccgtc acgatcgtcg
     4201 cggcgctggc gctggcgacg gcatggggcg tgccggcggc ctcggcgcag gaggcgaccg
     4261 aggctgtgcc ggcggacgcg ggctggtcct tcgcgttgac tccttacgcc tggttcatcg
     4321 cgatgaaggg cgacgtggcg acgttttcag gagccccgcc catcaacgtc gatgcggatt
     4381 tcagcgacat catcgaccag accaacggtg cagcactgct ggccggcgag gcgcggcggg
     4441 atcgcttcgg cctgctcttc gacatcaatt acctggatct gtcgggcaag ggcgacacgc
     4501 cgggagcgct cttcggcgac gccgagatgg acaaccggac actgttctcc gacatcgccg
     4561 gcttctacga ggtgggtcgc gacgcgctct tcagcctcga cgtctatgcc ggggcgcggc
     4621 tatggtatgt cgaaaccgag gtcaatctgc gcgccggcct gttgcccaac cgcaccgacc
     4681 aggacagcga agcctgggcc gacccgatcg tcggggtgcg cggccaggca cttctcggcc
     4741 ggggcttttc cctgcagggc gccgccgact acggcgggtt ccacgtcggc tcgaagaaca
     4801 cctggcaggt gatcggaacg ctcggctacg acttcaacga ccggctctcc gcgcgggccg
     4861 gctatcgcta tctgtccgtc gactacgatg acaacggctt cgtctgggac gtcgacattc
     4921 acgggccgat catcggcctc acctggcgtt tttgaggcaa ccatggaaag caggggagac
     4981 gcggctatga ccggcaagca ccatctcgtg ttgggcctgg gcctgatgct tatcgccgcc
     5041 ggcccggcgc aggcgcagca ggtcacgggc acattgggct cgccctcggc gacgaccacg
     5101 attccgggca accagctacc ggcgccggcg ccggcgttcg gcggcgtgat caaggatgac
     5161 gccctcagct ccacgccctg gtggccgccg cgcatcgtcc cgcccgaggg tgctcccaac
     5221 atcctgctga tcatgaccga cgatgccggg ttcgccgtgc cgagcacctt cggcggcgtc
     5281 atcccgacgc cgacgatgga tcgcatcgcc gaggccggcc tgaagtacaa ccgggtcttc
     5341 tccacctcgc tctgctcgcc gacacgcgcg gcgctgatca ccgggcgcaa ccatcattcg
     5401 gtcggcttcg gcgtgatcgc cgagcaggcg acgggctttc cgggctacga cagcgtcatc
     5461 ggcgtggaca acgccaccat cggccgcatc ctgcgggaca acggctacgc gacgtcgtgg
     5521 ttcggcaaga accacaacac gccatcgtac caggccagcc aggtcgggcc gttcgaccag
     5581 tggccgaccg gcatgggctt cgactacttc tacgggttcg tcggcggcga cgccaaccag
     5641 tgggggccga acctgttccg caacaccacg cagatctatc ccttcgacga acaccccggc
     5701 accctggaga tggatcgcag cgacgcggcg atctggccgg tgaccggcga ggagagctcc
     5761 tggaacctga tcacggcgat ggccgacgac gccatcgact ggatgacgcg gatccaccag
     5821 accgacccca gccagccgat cttccttcac tacgtgcccg gggcgaacca cgcgccgcac
     5881 cacccgacca aggagtgggt ggacaagatc cacgccatgc atctgttcga cgacggctac
     5941 gagaagctgc gcgagcgcat cttcgccaac cagcagaagc tcggcgtcat cccgcaggac
     6001 ctggagttga cgccctggcc ggacgacgtc ctgacgccct gggacaagct atcgcccgag
     6061 gcgcagaagc tctacatccg ccaggtggaa gtattcgccg cctacgtggc ctacaacgac
     6121 cacgagatcg gccgcgtggt ccaggccttc gaggacctcg gcaagctcga caacaccatc
     6181 gtcatctacc agaacggcga caacggcacc agcgccgagg gcgggccgga aggcaccttc
     6241 agcgaggtcg cgttcttcaa cggcgtccgg ccacccgtgg acgtgcagat gaagttctac
     6301 gacgcctggg gcaccgagct ggcctacaac cacatgtcgg ccggctggtc ctgggccttc
     6361 gatacgcctt tcgactggtt caagcagaac gccagccggc tcggcggcac caaccagaac
     6421 atggtgattt cctggcccga tcgcatcaag gacaagggtg gcctgcgcga gcagttcatg
     6481 cacgtgatcg actacgtgcc gacgctcctg gagctcaccg gcatcgaggc gccgaagctc
     6541 gtcgacggca tcgagcagaa gcccatcgaa ggcaccagct tcgcctacac cctggatgcg
     6601 gccaatgccg aggcgccgtc acggcacacg cggcagtatt tcgagatgat gggtcagtgg
     6661 gcgttgtacg acaacggctg gctgctgagc accaaggtca atcgcacacc ctgggatgcc
     6721 ttcagcccgg ccaaccggga tccgctcaac aaccaagtgt tccagctcta caatcttgaa
     6781 gagagctgga accaggccga cgacatcgcc gcggcacacc cggacaaggt cgaggcgatg
     6841 cgggcgatgt tcctcgagga ggccaagaag taccaggtcc tgccgctcga cgcctcggtc
     6901 ggcgcacggg tcgcggcaca gcgcccgagc ctcaccgcgg gtcgcacgga gctcgtctac
     6961 acccggccca tgaccgggct gccgcagggc gatgcccctt atctgctcaa cacctcctac
     7021 accatcacgg ccgacatcac cgtgcccgag ggcggtgccg agggcatgat cgtgacctcc
     7081 ggcgggcgct tcgcgggctt cggcttctac ctgctcgagg gcaagcccgt attcctctgg
     7141 aacctgctgg atctggagcg gcaaaaatgg gagggccaag acgcgctgga gcccggccag
     7201 cacaccattg agttcgactt cgtgtatgac ggcctcggcg tcggcacgat ggcgttcaac
     7261 aacttcaccg gcatcggcag gccgggcacc ggcacgctca aggtcgacgg caaggtggtc
     7321 gccacgcaga agatggaaaa gacgatcccc atcatcctgc agtgggacga gagcttcgac
     7381 atcggctccg acacgatcac cggggtgaac gacgcggact atcggccgcc cttcccgctg
     7441 accgccgagc tcgacaagct gacgatcagg atcgatcgcc cgcagttgtc gcccaaggac
     7501 atcaaggagc tcgaggaagc catgaagaag gtcgacgccg ccagggagtg agcgatgagc
     7561 ggggccggcg gccatgggtc gccggctccg tcgcgggcga gcaacgagca acccgatgag
     7621 ggaggacggc ctggtgatct cgacatccct gcccagatgc ctcgcgctgg tcctggcggt
     7681 tcagttgctc acgatggccg cagcgcgggc ggaggagccg gcagcgccgc cggcggcacc
     7741 gcccagcacg ctcgatctcg ccaagaaggc ccagaacccg gtggccgacc tgatcagcgt
     7801 gccgctgcag agcaacttca atttcggcta tggcgcgaag gacgcgcccg agtcgagcag
     7861 cacccagtat ctgctcaaca tccagccggt gatcccgatc tcgctggggc agagcggctt
     7921 caacctgatc acccggccga tcgtcccggt catccgccag cccgacctga tcgagggggg
     7981 cgagacctgg ggcgtcgccg acatccagat ccagagctac ctgtcgccgg cgggctcgaa
     8041 ggagctgatc tggggtgtcg gcggcgtgct gcagccgccg accgcgaccg acggggacaa
     8101 gctcggcacg cagaaatgga gcgccgggcc ggcggcggtg gtgctgtcca tgccgggcaa
     8161 gtgggtgttc ggtgccctgg cgacgcagct ctggtcgttc gccggcaaga gcgaccgcga
     8221 ggatgtgagc cagacgctgg tgcagccctt catcaactac aatttcgacc atggctggta
     8281 cgtctcctcg gccccgatca tgacggcgaa ctggaaggcg gacggcaacg acaacacctt
     8341 caccgtgccg atgggcggcg ggttcgggcg gctcattcgg gtcggcaagc tgcccgtcaa
     8401 cctgcaggcg caggccttct acaacgtggt caagcccgac gacgatccga ccgccgactg
     8461 gacgctgcgg ctgcagatgc agttcctgtt cccgaagtga gagaagcaaa tggacaagag
     8521 catccatagc ccagccgcaa cgcctgtgcg ggcgggcagg tgcgtcacct cggcgatcgt
     8581 cggcgcgatg gcgatgaccg gcatgctcgc cctgcagccg ttcaccgtgc gggcgcaggc
     8641 gctggacggc ccgccggcga tgccggcctt cgagttctcg acggcgatgc cgtccggcgt
     8701 cgcctcaccg gacgaggtcg agtcgcggct cgggacgctg aagttcttcg acggcttccc
     8761 cgacaaggcg acggccgagg tggtctacga taatctcgac ttccagcgtg cggtgcaggc
     8821 ctatctgctg gccctgccgg cggtcaacca ggcgggcaac ctgaacgcga tcgcgtcgct
     8881 cggcccgttg aacgcgaccg tcccgatctg ggaggagctg gtcgactccc gcacgattga
     8941 gctgacggcc aacgacaaca ccccctacac ctggttctgg atcgacctca ccgacggtcc
     9001 gctggtcgtc gaggtgccgc ccgaggtgct cgggctggtc gacgacatgt ggtaccgctg
     9061 ggtgatcgat gtcgggatca cggggccgga caagggcaaa ggcggcaagt acctgttcct
     9121 gccacccggc ttcgcaggcg acgtgcccga aggcgacttc gtcgcggtgg tgcatgcgac
     9181 cacctaccgg ctctggatcc cgtggcgcag cttcctcgag aacggcgacc ccaagcccgg
     9241 cgtcgagcgg gtcaaggcgc acaccaggat ccatgcgccg ggatcggagc ccgtggcacc
     9301 gaccttcgtc gatatgtcgg gcaagccgtt caacatggtc aacccggcgg actatgcgtt
     9361 ctgggagctg ctgaacgacg tcgtccagaa ggagccggca ggctcgctgg acgaggtgac
     9421 cctcgggttc ttcgccgcga tcggcatcga gaagggcagg cccttcgcgc ccgacgatcg
     9481 catgaagaag atcctgaccg aggcggccac ggtcggcgat gccacggccc gcacgatcgc
     9541 cttccacatg cgcgaccgca atgcctacta ctatgcggac agcaactggc agctgccctt
     9601 cgtcggcggc tacaggttcc agtcctcgcc cggcgtgctc aacctcgacg gttccgcgtt
     9661 cttcttcttc atggcgacgg gcgtgacccc ggcgatggag gagaagatgg tcggtcgggg
     9721 ctcgcaatat gcctggacgg cgcgcgacgc cgagggcgaa ccgctcgacg gcggcaagag
     9781 ctaccggctg catctgccga aggacatccc ggtcaaggat ttctggtcgg tgatcctcta
     9841 cagcgaccag acccgctcga tgatccagac cgaccagcgc ttcccgagcg tcagcagcca
     9901 gacccagggc ctgctggtca acgccgacgg ctcggtcgat gtctggttcg ggcccgaggc
     9961 gccgagcggc aaggagaaga actgggtcca gacggtgccc ggccaggcct ggaacaccat
    10021 cctgcggctc tatggcccgt tggagccctg gttcgatggg tcgtggaagc caggcgagat
    10081 cgagccggtg ggctgagccg ggcgagacag ggaatggaca gcaacagcaa ccgggcatgc
    10141 tcgcgccgat gcgggccacc cccaaggagg aatgatccaa tgccgaacac gacgacgtcc
    10201 gcatggcggc tcggcgcgat cggggcggtg gcgctactcg ccgcctgcac ccagagctcg
    10261 agccagatcg agacgacggc ggccttgccg aagccgcagg tgatcgtggt ccaggacttc
    10321 gccgtccagg ccggcgaggt gcagctcgat ccgggcctca gcggcacgat cgacgagacg
    10381 atcaaggcga gcacgctgcc gccgaacacg gccgaggaag cccaggtcgg ccgtcaggtg
    10441 gcggatgcgc tggcgcagaa gctggtgacc gagatcaacg atctcgggct gaatgcgcag
    10501 cgcggcaaca ccctgcccga cggcatgtct tcgggactcg agatcaccgg ccagttcgtc
    10561 ggcgtcgacc agggcaaccg gaccgagcgc gtcgccatcg gcctcggcgc cggtcgctcc
    10621 gacgttcgcg tgcgcgccca ggtcttcgag gtgacgccga gcagccggac gctcgtcgac
    10681 gagatcgagg tcgacgccaa gagcggcctg cagccgggca tggccgagat gatgggcgtc
    10741 ggcgcgctgg ccgggcattt cgtcgcgtcg accgtggtca gcggcgggct gcaggtcgcc
    10801 gacgaatcgc tcggcacctc ggtggtcgcg gattccgacc gggcggccaa aggcatcgcc
    10861 aagcagctcg cgacgttctt cgccaagcag ggctggacga attgagcttc gggacgaaat
    10921 tcgagcgctt ccgggccttt cccgcgagca gctccagaag gaagggagaa acgatggcca
    10981 ggatgatcgg gctgtccctc gccgctgcca tcgggctcac cgccgcgatg gcctcggcgc
    11041 aggaaggcat gaagatcgcg cgtgcctgcg gcgacgacat cgacaggctc tgcgccggcg
    11101 tgccgcccgg cgaaggccgc atcaaggcgt gcatgaaggc gcacatgacc gagctgtcgg
    11161 cgccgtgcat ggacacgctc ctggctgcga tcgctgccga gaaagagccg gagtgaccgc
    11221 ccggtggcgg ctgccgtcgc gggcgcccgc ccgtggcggt ggtcgccccg gctcgaggcg
    11281 aaagggaaac ggcatggacg tgcaagctcg gcgcgaccgg gatcggccga ggtggtttcg
    11341 gatgcttctc ctggccgcgg ccagctttct cttgacccat gtagccgtgg cgcaggaagc
    11401 gcagagtcgg ccgcagcgcg agttcgcgct gccggtgacg ccggagctcg agccgggcgc
    11461 catggagctc ctgcaggcga tgggggagcg cctggccgcg gcccgaacgt tgagcttcac
    11521 cgccgtcgcg acctacgaga gcccggcgcg gaccgggctt cccctggcct acaccacgct
    11581 gtcggaagtc acgctgcagc ggccggatca tttgcgggtc gtcacgcccg gcgacgggcc
    11641 ggcgatcgag ttctactacg acggggcgac catgatggcc tatcagcccg atgccgggct
    11701 gtcggccatc gccgatgcgc cgccgacgat cgatgccatg ctgcagaccc tgttcggtct
    11761 ctcggcgacc tattttccgt tcaccgacgt gatcgtggcc gatccttacg gcgacctcgc
    11821 aaccggactg cagctcgcct tcgtcgtcgg ccagtcgacg gtggtcggcg acacgctcac
    11881 cgacatcgtg gtgatcgccg atccgacgct gcagatgcag ctctggatcg gtgccgagga
    11941 ccggttgccg cggcgtatcc aggccagctt cttcgacgag ctcggccatt tccgccacgt
    12001 tctggagctg tcggactggc ggctcgatcc cgagctgccg cccgacacct tctcctccgc
    12061 cgccgcggcg gcggcgacac caatcccgtt tgccccgccg gggacgccct gaagatggag
    12121 ccgcgccgat gatgagactg gcaattcttg ctctggtcgg tgccgcggcg attgtcctgc
    12181 cgcgagaggc tggcgcctgg gctcatgccg gccgcttcgg cgtcgcgtcc ggcggtgggg
    12241 gctcttggca cgccgacggt tggcgcggcg gcagtgcctc gggcggcgac ggctcgtggc
    12301 atggccaggc ctggcgcggc ggcagcgcct ccggcggcga cggctcgtgg catgccaccg
    12361 gtgccgccgg tgggacgggt tccggcggcg acgggtcctg gcacgccaac agcgcctggg
    12421 gcggcagcgc ctcgggtggc ggcggctcct ggagcgccca cggtgccgag ggcggctctg
    12481 cctcgggcgg cgacggctcg tggcatgcca ctggcgcgga gggcaccaag gttcagggca
    12541 gctatgctca cgcgaccagc gtcgacgtcg cctatcccca cgcctatcgg ggcccctact
    12601 acgccaccta tcacccgccg gtgaccgtcg atcactacag cagcacttgc gacaattgcg
    12661 gcggctggtc cggcggcgcc gtcgccgcgg cgggtgctct cggcctagcg gctggtgcgg
    12721 cgatcgacgc gtcggcggcc aaggcggaca ccgcggccgc caccagtagc gcctatgcgg
    12781 cgggagtctc cgccggcgag gcggcggccc ctgcctcggc tgccacgagt cccgcccagc
    12841 cgaccaagac ggcggcggcg ccgagcggga ccgtctacac cgagctgccc gccaattgcg
    12901 cctacaaggt ggtgaaaggc agcaactact tcgactgcgg cgacatgtgg ctgaccgccg
    12961 cctatggcgc caacggcctc cactacacgg tcgtgccggc cccttgactg ccggccctgc
    13021 gagctgcccg gtcgggtgga gatgacggcg gaggagagcc tctgggtcgc tgactggctc
    13081 tggagcctgc ctttgatcgc cttctgcgtg gccatccacg tcgttggcct cgccctgatc
    13141 aacgatcgcg tggtcggctt ccttcgcgac gccatcgaca atcgtaattt cctgcggctg
    13201 ttcgccgtcg tgatcggtgc cgtggccctc ctcgccacca cgctgcacgg cctcgaggcg
    13261 tggctctggg cgctcgccta ctggctgctc ggcgcgcttc ccgacttccg ctcggccatg
    13321 ctctactcga tcagcgcgat gaccacctac gggcattccg atatcagtct cgaggcggaa
    13381 tggcggatga tgggcgcgct ggaatcgctg tgcggcatgc tgctgttcgg cctgaccacg
    13441 gccttcctgt tcgcggcgat ccagaaggtc tggccactgg gagcccgcga ccgcaggctg
    13501 taggccgttg ccggggtgga ccgtcgacga cgccttccgc tatggaatcg ggggcggccc
    13561 ggaactgcga atgaggtcgc cgggccgggc ccaagcctgg ccgggccgcc aaggccgtgc
    13621 aggcgacgcg aggagcacag catgacgaga ctctcgacca tggcggtcgc ggcggggatc
    13681 gctttggcca gcggggcgca ggcccaggac atctcgaccg agctgccgcc ggaggatcgc
    13741 tggacggtca cggtcgcacc ctatctctgg atgctgtcgc tcgatggcaa caccacggtc
    13801 ggcggcatca aggccgacgt cgacgtgccg ttcaaggatt cgatccagga cctctccttc
    13861 ggcggcatgc tgctcacgac cgtgcgcaag ggaaagctcg gcttctcgat caatggcgtc
    13921 tatacgcgtg tttcgcccga caactcggtc ggtccgctcg acatcgattc gaccgccgat
    13981 ctggcgcagc tcgccggcgg accgttctat cgaatggtcg aatggcagta tggcgagacc
    14041 tctgccggcc agccgctccg gttttacctc gagccctatg tcggagcacg gctgaactat
    14101 cttcgggtcg aggtcgaggt gcgcggcggc cagcagcagg acgagagcca gacctgggtc
    14161 gacccgatcg tcggctcgcg ggtcgggctc gatctgaccg accgcctggc gctcttcggc
    14221 ggggcggacg tcggcggcgt agtcaccggc tcggatttcg cctggaacgc gcaggcctat
    14281 ctcggctacc agacgagcgt cttcggccgg gagacgttcc tgtcgttcgg atttcgcgcc
    14341 tatcatgccg actacgaggg cggcaacttc aagtgggatg cgacccagta cggcccgatc
    14401 atcggcaccg cgatcaagtt ctgagcgagg ccgttccccc gacggactgt cggcgacgcg
    14461 ggccgttcag tcggcgagac cgccccgcat cttgcggcgc atcacgccgc cgacgacgat
    14521 cggcaggatg aagccgatga tggcgatcgc ccagagcacg agggcgagcg gcgaggcgaa
    14581 gaggatgccc cactcgccgt ccgagatggt cagcgcccgc cgcaggttcg cctccatctg
    14641 gttgccgagc agcacgccga ggatgatcgg cacgagcggc acgtcgagct tcctgaggac
    14701 ccagccgagc aggccgaaga cgaccatcac gaggagatcg aagctcgagc ccgagatgcc
    14761 gtagatgccg acaaaggaga ccatggcgac gatcggcatc aggatgcgcg gcggcaccag
    14821 cagcacgcgc acgaacatgc cgaccatggg gatgttcatc gccagcagca tgaagttggc
    14881 gatgaagagc gcggcgatca ggccccagac cacgtccggg ttctgctgga aaaggagcgg
    14941 gccgggcgtg atgttgagcg ccagcagcat ggcgaggagc acggccgtgg tgccggagcc
    15001 gggcacgccg agcgccagca tcggcaccag cgccccgccg gccgcggcgt tgttgccggc
    15061 ttcgggggcc gccacgccac gcgggtcgcc cttgccgaag gtgccctcgc ggtcgaccag
    15121 cttcttctcg aaggaatagg cgatgaagga cccgagcgag gcgcccgcac ccggcagcac
    15181 accggcgacg aagccgagtg ccgagcctct gagcatcgtc ggcgtgcact tgcgcagcac
    15241 cgacatggac ggtgtgatgc gaccgatgcc gaccgtggcg gccttgccct cggccgtctc
    15301 cttgccgcga tgctcgagga agacgaagac ctccgaaaga gcgaagaggc cgacgatggc
    15361 gacgaggaaa tcgatgccgt cgtagagatg cacctcgccg aaggtgaagc gcggcacgcc
    15421 ggtctgtccg tcgacgccga ccatggcgat gccgacgccg agggcggcgg cgaaggcgga
    15481 cttggcctgg ttggacgacg agatgccgcc gagcgtggcg aaggccaggg tgaagagcgc
    15541 gaagtactcg gccgggccga agagcagggc cagcttgacg agctgcgggg cgaggaagac
    15601 gaggccccag gtcgagagga aggcgccgac gaaggaggcg acgcccgaga gcgccagcgc
    15661 ctcgccggcc cggccctgct gggccatggg atagccgtcg aggcaggtca tcatcgccgg
    15721 ctcgtcgccc gggatgttca ggaggatcga cgagatgcgc ccgccataca tggcgccgaa
    15781 atagaccgag gtgagcagga tcatggccgg cgtcgccggc aggccgaggg tgaaggcgag
    15841 cgggatcagg atcgccacac cgttcgacgg gccgaggccc ggcagggcgc ccatgacggt
    15901 gccgagaaag cagccgagca gggccagcgc catgttctgc caggtgatgg cgatccagaa
    15961 gccgtcgccg agggagccga gcacatccat ggcggggcct cagaagccga gggggccgcg
    16021 ggcgagcgat agcccgaggg cgagcgcgaa gaccgcgtag atgccgagcg agatcgcgat
    16081 gccgacgacg aacgaccaga gcggcacggt gccgagccgc caggtgagat aggtggcggc
    16141 cagggccgtg gcgatgacga agcccagctc gggcagcagc agggcgtagg cgaccatgac
    16201 cgcggcggcg acggcgatct cgaggaggcg cgtggcgcgc ggccagtcgg gtgctgcatc
    16261 cgggcggatg aggatgacga ggctggctag gccgagcacg acgccgacga tgatggggaa
    16321 ggtgcgcggc cccaccgggt cggagatgaa gctgagctgg atctgggtcg cctgccagat
    16381 gaagaagaca gcgagcgcga gcccgatgcc gccgaagacg cggtcgctca tgacttggtt
    16441 tccgtctcgc ctggcaggtg ccgcggggcc cgggcacgca gcctcgtgga ccgcatggcc
    16501 gggccgctcg ccgctactgg atcaggccga tctccttgga gagcccgttg atccggctca
    16561 ccgattcggc gacgaaagcc tcgaactcgg ggccgcgcag gtccaggggt gcgagaccat
    16621 tctgcgccat gatggacttc cattcgtccg agtcgtagac gctgccgatc gactcgaccc
    16681 agaagtcgta tgcctcgtcg gacatgttgc ccggtgcata gaagccgcgc cagttggcgc
    16741 cgaccacgtc gaggccctgc tcggcggccg tcgggaagct ggcgaagtcg ccctcgaggc
    16801 gctcgggggc gagcacggcg atcaccttga tgtcgcccga atcgacgaag cccttggcct
    16861 ccgacgcatc gcccgtgaac gcctgaacag agcccgcgag gagctgggtc acggcctcgc
    16921 cgccgccgtc gaaggcgaca tacttgatcg tccggacatc gtcgatgccg gccgcctggg
    16981 cggcgatcag caccttgaga tggtcccagc cgcccaccgc cgagccgccg gcgatggaga
    17041 cggacttcgg gtccgccttg atctggtcca tcagctcggg cagcgtcgcg atcgggctgt
    17101 ccttggcgac cgcgatgatg ccgtaatcgg cgcccacgct ggccagccag cgcacctggt
    17161 ccatggtgtt gcccgggtag gcgccctggg cgaggcgggt cgaggtcgcc gaggaggcgg
    17221 cgacgatcag gtcgttgtcg tcggcgcgct tgttgaccac ctcggcgaat gccacccccc
    17281 cgccgccgcc ggcgatgttg gtgacctgca ccgtgccgtc gacgagaccc agatcctgca
    17341 gggtcttgcc gacctgccgg caggtgaagt cccaaccgcc gcccggattg gccggggcga
    17401 tgcattccgg gttctctggc tcgaaggcga gcgctcgatc ggcgggcatg gcgccggcca
    17461 gcatggcgcc ggcaagcgcc ccggcggcga aaaggccgcg tgagtccaat gaaaatcgca
    17521 tgtgtccagc ctccactgac tgcttgcctc ggcggcaccc gcaagtcacc gccgttgatg
    17581 accgacgcta gcgcaagact cccgcaggag atagtgtgaa ttcccccacc cttgccggcg
    17641 gcgcttgaaa tggcggatcg aacctgccaa cgtccgcttg ccagggggga gcatgagcca
    17701 gaagaacgtg agagccgacg catgatcggt cgttgccggg actgctttct gtggcgacgt
    17761 tgacgatcga caggctgacc aagcgctacg gcga
//
LOCUS       DAOTFI010000096        17685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3846_length_17685_cov_7.428020, whole genome shotgun sequence.
ACCESSION   DAOTFI010000096 DAOTFI010000000
VERSION     DAOTFI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17685
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3846_length_17685_cov_7.428020"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="P5558_11380"
     CDS             complement(<1..218)
                     /locus_tag="P5558_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY24957.1"
                     /translation="MGKLDVVASKAFEAQLLAKLDSPEPVLFVDFAECAYVSSAGLRA
                     VLIAAKTAKKTDHRLVLAGMNPIVREVF"
     gene            complement(327..641)
                     /locus_tag="P5558_11385"
     CDS             complement(327..641)
                     /locus_tag="P5558_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24958.1"
                     /translation="MLPVAYRLSLAVSLVLLLSVLVTAALNFLKLNQVMQSLEDSRYR
                     FVTRDLKVAFEQNLDQGLPLDQIDNARAILERQLARDSAIAGAENFIRAAHGLVGPVS
                     RY"
     gene            complement(957..1448)
                     /gene="tsaA"
                     /locus_tag="P5558_11390"
     CDS             complement(957..1448)
                     /gene="tsaA"
                     /locus_tag="P5558_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRY24959.1"
                     /translation="MTDEDTIRPGEVAVEPPEASDAGLVFIGRIRTPWTTRASCPRQG
                     RLEGPLCRIELFHPWGMGLAGIERHERLEVLYWLDRARRDILLQSPARDGRTRGVFSL
                     RSPVRPNPIGTSIVRLERVEGLALLVRGLDCLDLTPLLDLKPDRTLFTPAAPAKSTVD
                     ETS"
     gene            complement(1454..2734)
                     /locus_tag="P5558_11395"
     CDS             complement(1454..2734)
                     /locus_tag="P5558_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY24960.1"
                     /translation="MYIAILFIAFFGLLGIGVPVAFALAGSSLVYVLVSGAVPDVVVL
                     HRMVNGIDSFPLLAVPFFILAGNLMNSAGITNRIFGFARALVGWMRGGLGHVNVGASV
                     VFAGMSGAAVADAGGLGTIEVKAMRDAGYDRGFSVGVTAASSTIGPIIPPSLPMVIYG
                     VMANASIGQLFAAGFIPGLLMAVSLMVMIAWYARVRNYPRDAAFDLCVLWHATQKAFL
                     SLMTPLIIVGGILSGMVTPTEAAIAAVFWALILGIFVYRTLTMTRLVRVSMETIETTA
                     SIMLIVAGASIFAWILTSNRVTEHFAALVLMISDNPIIILILLNVILLVAGCFLETIA
                     AITILVPVLLPVAISIGIDPVHFGVIMVLNLMIGLLTPPIGLVLYVLAKVTNIPFDVC
                     MRATLPFLVPLVVVLALVTFIPSLSMFVPNLVYR"
     gene            complement(2746..3288)
                     /locus_tag="P5558_11400"
     CDS             complement(2746..3288)
                     /locus_tag="P5558_11400"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY24961.1"
                     /translation="MQGEAPRNDEFGFHLEEKPDPPFEWREFGLEDWPAFLVFWGLTV
                     IVFLQFFTRYVLNDSFGWTEEAARYFLIGTTFIGGGMVCRRRAHIAVEYLHTRLSRAG
                     SRRLMIVVDVITIAFLAYLGWVGWRMIGMMAPQKMAVIDLSMSWLYSVVFIGIIIMIL
                     RTAQSLLHRLQGDSPYPGDI"
     gene            complement(3311..4291)
                     /locus_tag="P5558_11405"
     CDS             complement(3311..4291)
                     /locus_tag="P5558_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HRY24962.1"
                     /translation="MISMSRRALGASVAAAMALLAGGASAQQALKFAHVYETSEAYHT
                     AALWAAEQIAERTDGRYTMEVFPASSLGKETDINQGLTLGTVDIIYTGMNFAGRSFEP
                     LTIASAPYMFRDVDHWKAYAESDLWDEMKEGYVDATGHHVSAILYYGERHVTSKTPIN
                     TPEDMKGLKIRVPDAPLYVLFPNAVGANPTPIAFAEVYLALQQGTVDAQENPLPTILA
                     KKFYEPTPIINLTGHITESLITIISGNLWNSLSDEDKVIFDEVFQEAAIRSNEEIYAS
                     EAELVTGFAELGATVNEVDRSLFKAVVEPHLVGDDVPWSQEQFDRLQAIK"
     gene            complement(4410..6665)
                     /locus_tag="P5558_11410"
     CDS             complement(4410..6665)
                     /locus_tag="P5558_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY24963.1"
                     /translation="MRAWLIGLVLAGLATAAARAEVVIVYDASNSMWGQVEGRHKIEI
                     AREVLGELVADWDPAESLGLVAYGHRREGDCSDIEVVVQPGPVDPAAFVAKVEGLVPR
                     GKTPLTEAVRQAAELLRYRDVPATVLLLSDGIETCGADPCAVAAELAAAGVAFTAHVV
                     GFDVANEADQAQLRCIADNTGGLFRTAADAGELREALAQVAEAAVAAPVETQVQPEPE
                     PEPEALPEATLAAAASVDVATAIEVAWTGPGAPRDYVAIAGLDQRDGQYINYSYAEAG
                     SPLTVTAPAEPGLYELRYVEQASNAVLARLEIEVVDVPASLEAPEIVQAGSQVSVGWT
                     GPGHDRDYVSIAAADQNPGAYVNYTYVNAGSPLVVTAPADPGLYEVRYVLREGPRVIA
                     RRAIEVAAAAATLDAPLQVPAGSEVAIAWTGPGHDRDFVSIAAADQNPGAYVNYTYVN
                     EGSPLAVTAPADPGLYEIRYVLREGPRVIVRRELEVTPLTVTLDAGDSVAIGHPLEVS
                     WSGPGYDRDYIAIAEPGQRPGQYLAYTYVNEGSPLSVEAPLEPGLYELRYVLREGPRV
                     IGQREIEVVAATATLVAPDSVAPGQGFAVEIQGPLLARDEVVLVPADAPARTREHAQR
                     IGRGATLDFVAPGESGLYELRYLLHGGGPVIGRRPVEVAALAAALEAPAQVAAGSSID
                     VGWSGPGGEGDRITLAEPGSAPTKWRTAVRTAAGNPATLVAPAEPGSYELRYFSGESR
                     QIATQRGLIVE"
     gene            6803..8761
                     /gene="thrS"
                     /locus_tag="P5558_11415"
     CDS             6803..8761
                     /gene="thrS"
                     /locus_tag="P5558_11415"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRY24964.1"
                     /translation="MASAVEHAPDIAPGGIVITLPDGSERRFGSTTTGAEIAASIAKS
                     LAKQALAVEVDGVLRDLDWPIGTYAGVRIVTGRDPEALALIRHDCAHVMAEAVQDLFP
                     GTQVTIGPPIENGFYYDFARNEPFHPEDLERIEARMAEIVKADKPFRREEVSRAEAHR
                     RFEAMGERYKLELLDAIPEGEPVTLYHQGEWFDLCRGPHGPSTGRIGAFKLMKIAGAY
                     WRGDARNAQLQRIYGTAFASQKELDAHLHQLAEAERRDHRRLGREMGLFHIQEEATGS
                     VFWHPKGWRIWQEVESYMRRRLDAAGYQEVKTPQLVDRVLWERSGHWEKFREHMFTLD
                     ADERVLALKPMNCPCHVQIFNQALKSYRELPLRMSEFGACHRNEPSGALHGIMRVRAF
                     TQDDAHIFCTEDQVTAESVAFCELLRQVYADFGFTDVSVKFSDRPTVRAGEDATWDRA
                     EQALRDATEAAGLETVLNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDFVLPERLDA
                     TYVGADGARHRPVMLHRAILGSMERFLGILIEHHAGRLPLWLAPVQIVVATVTNDADA
                     YAREVAAALEGAGLRVDCDTRSDKISYKVREHSLAKVPLIMAVGGREAAEHSVSLRRL
                     GSQAQEALSLDAAIAMCQRESAAPDRQT"
     gene            8873..9397
                     /gene="infC"
                     /locus_tag="P5558_11420"
     CDS             8873..9397
                     /gene="infC"
                     /locus_tag="P5558_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRY24965.1"
                     /translation="MAANPKGDEHRINDRIDAREVRLVDQDGGMVGVVAIRDALTRAE
                     DVGLDLVEISPNATPPVCKILDYGKFKYEAQKKANAARKKQRVIEVKEIKMRPSIDDN
                     DYSIKMKKVREFLDEGDKVKVTMRFRGREMAHQHLAMNILEKVREELADLAKIEQFPK
                     LEGRQMIMVMAPLK"
     gene            9394..11178
                     /locus_tag="P5558_11425"
     CDS             9394..11178
                     /locus_tag="P5558_11425"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRY24966.1"
                     /translation="MTAPQAGRLQALRDALAGAGVDGFILQRTDQHGSEYLPAAEQRL
                     AWLTGFTGSAGQAVVLPDAAAVFTDGRYTVQIGIEVDASLFERCNSGDMPPPRWLEER
                     LQPGMRLGYDAKLIRKAEREKLARLCQGRGAELVALTPNPIDRIWTDRPPPPLAPVTA
                     HDESRAGESSVAKRQRMGEAVARRGADWLLVTAADSIAWLLNVRGGDVPYNPLCLSHL
                     LLGADGTARWFVDPAKLQGGTRLDNAVATEPYEALDGALAELGRSGARVLVDPTMNAA
                     GFADILAQAGARVVEGQDPIPLAKARKNPTEIEGALEAQRRDGAAVVRFLAWLDGLDH
                     DHAIDEAAAAERLEAERAKDPLYRGPSFETISAHGPNAALPHYRVTLDTNRKLTAGTV
                     YLVDSGGQYLDGTTDITRTVALGQPTAEVQERFTLVLKGHIALAEAVFPEGTSGSQLD
                     TLARLALWRRGLDFDHGTGHGIGSYLCVHEGPQRIAKRGGDTPLETGMIVSNEPGYYE
                     PGEYGIRTENLILVEEAPAAEGGDRRLLHFRTLTLAPIDRRLIDPHQLTEPERRWLDT
                     YHARVRDELRPLLDDVTGNWLKGATAPL"
     gene            11327..12784
                     /gene="ccoG"
                     /locus_tag="P5558_11430"
     CDS             11327..12784
                     /gene="ccoG"
                     /locus_tag="P5558_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRY24967.1"
                     /translation="MSEAVSVEDVQSRQEQRSSPLYKGRVEVYAKAVKGRFRTIKWAS
                     LATLLAIYYLAPWIRFDRGAGAPDQAILADMAGRRLYFFWIEIWPQEVYFLTGILVLA
                     AFGLFFATALFGRIWCGFTCPQTVWTDLFMAVERWIEGDRNERMRFDKQPWTAAKWRK
                     RVTKHAVWLLIAFLTGGAWILYFNDAPTMIGEFFSGRAGAAVYFFTFLFTATTYLLAG
                     LAREQVCTYMCPWPRIQGALVDQDTMAVTYEAWRGEPRGKHKKGQSWDGRGDCVDCKQ
                     CVAVCPMGIDIRDGFQLECIGCGLCIDACNEVMAKVERPPNLIAYDSERNQELRAAGQ
                     PERYRIVRPRTILYTAIIAAVALIMLGALALRSSTDVNVIPERNPLFVTMADGSIRNG
                     YQIKILNKERADRTYTLALADPMPGAVFSTPNGPSGSSLDLAAVSDDVATHRVFLGLP
                     RAALTEAATPITLVLTDNLTGEERRFSTVFRGPDT"
     gene            12781..13284
                     /locus_tag="P5558_11435"
     CDS             12781..13284
                     /locus_tag="P5558_11435"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRY24968.1"
                     /translation="MSVGLSGPATRHRPGWYIPWILVAGFGVVLAVNLAMIWIAMSTW
                     TGISTNRAYDKGITYNRNLDAAERQAALGWRASIVTAQAAGLAGTISVHLVDARGQPL
                     ERIDVQADFERPTHQGYDFALTLERTGPGTYAARFTAPLAGLWDVRVIATRGGDRLVV
                     TERLMLH"
     gene            13289..15511
                     /locus_tag="P5558_11440"
     CDS             13289..15511
                     /locus_tag="P5558_11440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY24969.1"
                     /translation="MVAHAAHSSGRSGAADDSAEKPTDVTGFVRIDPASGTAILQLMV
                     EGVHCGACVRRIERALEAEPDVQTGRLNLTTRRLTLAWEGPPARGNELVAKIEGLGYP
                     VVPFDPESLAVSDNRAERDLLRCMAVAGFAASNVMLLAVSVWAGHFSGMGDATRDFLH
                     WMQALIVLPSLAFAGRPFFRSAMAALRAGHANMDVPISLALVLAPAMSLLETIQGGEH
                     AYFDSAIMLLFFLLIGRYLDSRARGVARSAVERLMTLRATAVTLLLPDGQTRSCRPEQ
                     LEAGQTVLVAAGERIGIDGAVRAGQSEIDTSAITGETLPAIATPGTRVFAGTINLAAP
                     IEVTVRSVGEGTLLAEIVKLMELAEQKRGRFVALADRIARAYSPVVHLLAALTFLGWW
                     LAMDAPWQVALLNAVAVLIITCPCALGLAVPAVQVLASGRLMRRGVLLKSATALERLA
                     EVDTVVFDKTGTLTEGDLVLVEGNGEQDLLVQAASMARSSRHPLARALARACPGARAA
                     AAVEELPGLGLRLTTEAGELRLGRRSWAAPGIADDADAGTELWLALPGRPPHRFAFRD
                     RPRTDAAATVRALQARGLEVQLLSGDRPRPVAALAAELGIATWQAGCRPDEKVAHLAA
                     LAAAGRKVMMVGDGLNDAPALAAAHVSLSPSTAVDISQTAADAVFQGRFLAPVVLVLE
                     IAGRSRRLVEQNLGLAFGYNVLAVPLAVLGFVTPLIAAICMSASSLVVVGNSLRLNRK
                     "
     gene            complement(15538..15741)
                     /locus_tag="P5558_11445"
     CDS             complement(15538..15741)
                     /locus_tag="P5558_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24970.1"
                     /translation="MNDLGRWLGGIAMGVLALLGLIIMSRAADTMFGFFGVMIFLFGI
                     AIIVVFVHQATTPERVLRRTHDA"
     gene            complement(15775..16563)
                     /locus_tag="P5558_11450"
     CDS             complement(15775..16563)
                     /locus_tag="P5558_11450"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRY24971.1"
                     /translation="MADAVPVFAQPTVRVRKLEMDQPWTWLRKGWQDCLKTPGVSFVY
                     GTLAAVTGYLITWGLFAFDMPYLVLPIMAGFLIVGPILAVGLYEASRRSEQGLPTSLA
                     DALGAFNRNPSQMGLMGMALLLLMFFWARTAALLFFLYFGMEPPDFHNLFVATFLSAD
                     SWPFLIIGSAVGGVLALVAFSISVVSIPLLLDRPAANIIEAIATSVRAVQANPMPLLF
                     WGGLIVIFMMAGLVTLYLGLIVTLPLIGHATWHAYRDIVELDAA"
     gene            16853..>17685
                     /locus_tag="P5558_11455"
     CDS             16853..>17685
                     /locus_tag="P5558_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRY24972.1"
                     /translation="MAAVMTAPVGRALSAYDEDVVRLFTLATVFWGVVGFLVGVVIAF
                     QLAYPVLNLDLEWTSFGRLRPLHTSAVVFAFGGNALLGTSLYVVQRTCRTSLFGGPLL
                     ASFLFWGYQLFIVLAATGYVMGVTQSREYAEPEWYVDLWLTVVWVTYLVIFMGTLLKR
                     KEPHIYVANWFYLAFIITIAMLHIVNNLAVPVTFFGIKSYSLFAGVQDALTQWWYGHN
                     AVGFFLTAGFLGMMYYFVPKQANRPVYSYRLSIIHFWALIFLYIWAGPHHLHYTALPD
                     WA"
BASE COUNT         2917 a         6028 c         6020 g         2720 t
ORIGIN      
        1 ttgaagacct cgcggacgat cgggttcatg ccggcgagga ccaggcggtg gtcggtcttc
       61 ttcgcggtct tggcggcgat cagcacggcc ctgaggccgg cgctgctgac ataggcgcac
      121 tcggcgaagt cgacgaagag cacgggctcg gggctgtcga gcttggccag gagctgggcc
      181 tcgaacgcct tggacgccac cacgtcgagc ttgcccatgt tgacgatgag tgagaagcga
      241 tcgaggttgc ggatcggcag ctccccgggc aggcggccgt cgatcaggat ctgctccgcc
      301 agatggccga catactggcc gccattttag tagcggctga ccggcccgac gagcccgtgc
      361 gcggcccgga tgaagttctc cgcaccggcg atggcggagt cccgggccag ttgccgttcg
      421 aggatggcgc gggcgttgtc gatctggtcg agcggcaggc cctgatcgag attctgctcg
      481 aaggcgacct tcaggtcacg ggtgacgaac cggtagcgcg agtcctcgag gctctgcatg
      541 acctggttga gcttgaggaa attgagggcc gcggtgacca ggaccgagag cagcagaacc
      601 agcgaaaccg cgagactcag gcggtaggca accggcagca cagcccctcc cgcgtgccgc
      661 cggtggccgg gacggcgcgt ccgacgcacg cttccccgcc ggccggtagc cggcgagcac
      721 cccctcgtag tcaacgtagt cgaccagctt ccagccggcc agacgaaagc gttcaaaagc
      781 cttgcgatcc ggcaacctat ggtagtgaag gcgccccccg tcctgggttt cgccaccggg
      841 cctgcgaccc cgcgcgagat tggcgcaggc ctcgagcgcg gcatcgcccc ccggcgggta
      901 gaaatcgcag agcgctggtg gccacgatcc tgctatccat cttggattga tggacttcag
      961 ctagtttcgt caaccgtaga ttttgctggg gcagccggcg tgaagagggt ccggtcgggc
     1021 ttcagatcga gcagcggcgt caggtcgaga caatccaggc cgcgcaccag cagggccagc
     1081 ccctcgaccc gctccaggcg gacgatcgac gtgccgatcg ggttggggcg aaccggcgag
     1141 cggagcgaga acacgccccg cgtccggcca tcgcgggccg ggctctgcag gaggatgtcg
     1201 cgacgcgcac ggtcgagcca gtagagcacc tcgaggcgct cgtgccgctc gatccccgcg
     1261 aggcccatcc cccagggatg gaagagctcg atccggcaga gcggtccttc cagccgcccc
     1321 tggcgcgggc agctcgcgcg tgtcgtccag ggcgtgcgga tgcggccgat gaagacgagc
     1381 cccgcgtcgc tcgcctcggg cggctcaaca gcgacctcac ccgggcggat ggtgtcctcg
     1441 tcggtcatga aggtcagcga tagaccaggt tcggcacgaa catcgagagg gagggaatga
     1501 aggtgacgag ggcgaggacg acgaccagcg gcacgaggaa cggcagggtc gcgcgcatgc
     1561 agacgtcgaa cgggatgttg gtcaccttgg cgagcacgta gaggacgagg ccgatgggcg
     1621 gcgtgagcag gccgatcatc aggttgagca ccatgatgac gccgaaatgg acggggtcga
     1681 tgccgatgct gatggcgacg ggcagcagca cgggcaccag gatggtgatg gcggcgatgg
     1741 tctcgaggaa gcagccggcc accaggagga tgacgttcag gaggatgagg atgatgatcg
     1801 ggttgtcgga gatcatcagc acgagcgccg cgaagtgctc ggtcacccgg ttcgaggtca
     1861 ggatccaggc gaagatcgag gcgccggcga cgatcagcat gatcgaggcg gtggtctcga
     1921 tggtctccat gctgacccgc acgaggcgcg tcatcgtcag cgtccgatag acgaagatgc
     1981 cgaggatcag cgcccagaag acggcggcga tggcggcctc ggtcggcgtg accatgccgg
     2041 agaggatgcc gccgacgatg atcaggggcg tcatcagcga gaggaaggcc ttctgggtgg
     2101 catgccagag gacgcagagg tcgaaggcgg cgtcgcgcgg gtagttccgc acccgggcgt
     2161 accaggcgat catcaccatc agcgagacgg ccatcaagag gccggggatg aagccggcgg
     2221 cgaaaagctg gccgatcgag gcgttggcca tgaccccgta gatgaccatg ggcagcgacg
     2281 gcgggatgat cggcccgatg gtcgagctcg ccgccgtgac gcccaccgag aagccgcgat
     2341 cgtagccggc gtcgcgcatc gccttgacct cgatcgtccc gagcccgccg gcatccgcca
     2401 cggcggcacc cgacatgccg gcgaagacca ccgaggcgcc gacattgacg tggccgagcc
     2461 cgccccgcat ccagccgacg agggcgcgcg cgaagccgaa gatgcggttg gtgatgccgg
     2521 ccgagttcat caggttgccg gcgaggatga agaacggcac cgcgagcagc gggaagctgt
     2581 cgatgccgtt gaccatgcgg tggaggacga cgacgtcggg cacggcaccc gagaccagca
     2641 cgtagacgag cgacgagccg gcaagcgcga aggcgacggg cacgccgatg ccgagcaggc
     2701 cgaagaaggc aatgaagaga atggcgatgt acaagtggcc ggccctcaga tgtcgccagg
     2761 ataggggctg tcaccctgca ggcgatgcag cagagactgc gcggtgcgca ggatcatgat
     2821 gatgatgccg atgaaaacga ccgagtagag ccaggacatc gagaggtcga tcaccgccat
     2881 cttctgcggc gccatcatgc cgatcatgcg ccagccgacc cagccgagat aagcgaggaa
     2941 ggcgatcgtg atgacgtcga ccactatcat cagccggcgc gagccggcgc ggctgagccg
     3001 ggtgtgcagg tactcgacgg cgatatgcgc acggcgccgg cagaccatgc cgccgccgat
     3061 gaacgtggtg ccgatgagga agtagcgggc cgcctcctcg gtccagccga agctgtcgtt
     3121 cagcacgtag cgggtgaaga actgcaaaaa gacgatgacg gtgaggcccc agaagacgag
     3181 gaaggccggc cagtcctcga ggccgaactc gcgccactcg aacggcgggt cgggcttctc
     3241 ctcgagatga aagccgaact cgtcgttcct cggggcctca ccctgcatca taacgctccg
     3301 cgatgccggc ttacttgatc gcctgcaggc ggtcgaactg ctcctgcgac cagggcacgt
     3361 catcgccgac cagatgcggc tcgacgacgg ccttgaagag cgagcggtcg acctcgttga
     3421 cggtcgcacc cagctcggcg aacccggtca cgagctcggc ctcggatgcg tagatctcct
     3481 cgttcgagcg aatggccgcc tcctggaaga cctcgtcgaa gatcaccttg tcctcgtccg
     3541 agagcgagtt ccagagattg cccgagatga tggtgatcag gctctcggtg atgtggccgg
     3601 tgaggttgat gatcggcgtc ggctcgtaga acttcttcgc caggatggtc ggcagcggat
     3661 tctcctgggc gtcgacagtg ccctgctgca gcgccaggta gacctcggcg aaagcgatcg
     3721 gcgtcgggtt ggcgccgacg gcattcggaa agagcacgta gagcggcgca tcgggcacgc
     3781 ggatcttcag gcccttcatg tcctcgggcg tgttgatcgg tgtcttcgag gtcacgtgac
     3841 gctcgccata atagaggatg gcggagacat ggtggccggt cgcgtcgaca tagccctctt
     3901 tcatctcgtc ccagagatcg ctctcggcgt aggccttcca gtgatcgacg tcgcggaaca
     3961 tgtagggggc cgaggcgatg gtcagcggct cgaaggagcg gccggcgaag ttcatgccgg
     4021 tgtagatgat gtcgaccgtt ccgagggtaa ggccctggtt gatgtcggtt tccttgccca
     4081 gcgacgaggc cgggaacacc tccatggtgt agcggccgtc ggtacgctcg gcgatctgct
     4141 cggcggccca cagggccgcg gtatggtagg cctcgctggt ctcgtagaca tgcgcgaact
     4201 tgagcgcctg ctgggccgag gcgccgccgg cgaggagtgc catggcagcg gcgacggaag
     4261 cgccgagagc ccggcgcgac atgctgatca ttggatttgc ccccctgatc gtgaacgttt
     4321 gggcgcggtc gcccgcgccc gggtagccca cttctagagg agcgttcggg ccttgggtat
     4381 ccccgccgtg caggccccgt gcggcacagc tactcgacga tcaggccccg ttgcgtcgcg
     4441 atctggcgac tttcaccgga aaaatagcgc agctcgtagc tgccgggctc ggccggtgcg
     4501 acaagtgtcg cgggattgcc ggcagcggtg cgtaccgctg tccgccattt ggtcggcgcg
     4561 ctgcccggct cggccagggt gatccgatcg ccctcgccgc ccggcccgga ccagcccacg
     4621 tcgatgctgc tgcccgcggc cacttgcgcc ggcgcttcca gcgccgcggc aagggccgcc
     4681 acctcgaccg gccgccggcc gatcaccggc ccgccaccgt gcagcaggta gcggagctcg
     4741 tagagcccgc tttcgcccgg cgccacgaag tccagcgtcg caccacggcc aatccgctgg
     4801 gcatgctcgc gggtgcgggc cggcgcatcg gcgggcacca gcacgacctc gtcgcgcgcc
     4861 aggagcggcc cctggatctc gacggcgaag ccctgccccg gcgccacgct atcgggtgcc
     4921 acgagcgtgg cggtcgcggc cacgacctcg atctcgcgct ggccgatcac gcgcggcccc
     4981 tcgcgcagga catagcgcag ctcgtagagc cccggctcca gcggcgcctc gaccgaaagc
     5041 gggctgccct cgttgacgta ggtgtaggcg aggtactggc cggggcgctg gccgggctcg
     5101 gcgatggcga tgtagtcgcg atcgtagccc ggcccgctcc agctcacctc gagcggatga
     5161 ccgatggcga cgctgtcgcc ggcatcgagc gtcaccgtca gcggcgtcac ctcgagctcg
     5221 cgccggacga tgacgcgggg accctcgcgc agcacgtagc ggatctcgta gagaccggga
     5281 tcggccgggg cggtcacggc gagcgggctg ccctcgttga cataggtgta gttgacgtag
     5341 gcgccggggt tctggtccgc cgccgcgatg ctgacgaagt cgcggtcatg gccggggccg
     5401 gtccaggcga tggcgacctc gctgccggcc gggacctgaa gcggggcgtc gagcgtggcg
     5461 gcggcggccg cgacctcgat cgcgcgccgg gcgatgacgc gcggtccctc gcgcagcacg
     5521 tagcggacct cgtagagccc gggatcggcc ggagcggtca cgacgagcgg gctgcccgcg
     5581 ttgacatagg tgtagttgac ataggcgccc gggttctggt ccgccgccgc gatgctgaca
     5641 tagtcgcggt cgtggccggg accggtccag ccgacgctga cctggctgcc ggcctgaacg
     5701 atctcggggg cttccagcga cgccgggacg tcgacgacct cgatctcgag gcgggccagg
     5761 acggcattgc tcgcctgctc gacatagcgc agctcgtaga ggccgggctc ggccggggcc
     5821 gtgacagtga gcgggctgcc ggcctcggca taggagtagt tgatgtactg gccgtcgcgc
     5881 tggtccagcc cggcgatcgc cacatagtcg cgtggcgccc ccggaccggt ccaggcgacc
     5941 tcgatcgcgg tcgccacgtc gacacttgca gcggccgcga gcgtcgcctc gggcaaggcc
     6001 tcgggttcgg gctccggctc cggctggacc tgggtctcga caggtgcggc gacggcggcc
     6061 tccgcgacct gggccagcgc ttcgcgcagc tcaccggcat cggcggcggt gcggaagaga
     6121 ccacccgtgt tgtcggcgat gcagcggagc tgcgcctggt cggcctcatt ggcgacgtcg
     6181 aagccgacga catgcgcggt gaaagcgacg ccggcggcgg ccagctcggc ggcgacggca
     6241 caagggtcgg caccgcaggt ctcgatgcca tccgagagca gcaggacggt cgccggcacg
     6301 tcccggtaac gcaggagctc ggccgcctgg cggacggcct cggtgagcgg cgtcttgccg
     6361 cgtggcacga gcccctcgac cttcgcgacg aacgccgccg gatcgacggg gccgggttgc
     6421 acgaccacct cgatgtcgct gcagtcgccc tcgcgccgat ggccataggc gacgaggccg
     6481 agcgactcgg cgggatccca gtcggcgacc agctcgccca ggacctcgcg cgcgatctcg
     6541 atcttgtggc gaccctcgac ctggccccac atgctgttcg acgcatcgta gacgatgacg
     6601 acctcggccc gcgcggccgc cgtcgcgagc cccgccagca ccagcccgat caaccatgca
     6661 cgcatcgcca gctcccctgt tcgcgttctg cgggaaccta gtcgggcggc cgagcaattg
     6721 acaagcgcgg acggcgttgg gcattgtgca ccgccgcatt gcggaaacac cccggatcac
     6781 accagcggaa cagatcggta ccatggcatc ggcagttgag cacgccccgg acattgcacc
     6841 gggcggcatc gtcatcaccc ttcccgacgg cagcgagcgt cggttcgggt cgacgacgac
     6901 cggcgccgag attgcggcct cgatcgccaa aagcctggcc aagcaggcac tcgccgtcga
     6961 ggtcgacggg gttctgcgcg atctcgactg gccgatcggg acctatgccg gggtgcggat
     7021 cgtcaccggc cgggaccccg aggcgctggc gctgattcgc cacgactgcg cgcatgtcat
     7081 ggccgaagcg gtgcaggacc tgttcccggg cacgcaggtg acgatcggcc cgccgatcga
     7141 gaacggcttc tattacgact tcgcgcggaa cgagccgttc catcccgagg acctcgagcg
     7201 gatcgaggcg cggatggccg agatcgtgaa ggccgacaag ccgttccggc gcgaggaggt
     7261 gagccgcgcc gaggcgcatc gccggttcga ggcgatgggc gagcgctaca agctcgagct
     7321 gctggacgcg attcccgagg gcgagccggt gacgctctac catcagggcg agtggttcga
     7381 tctctgccgc ggcccccacg gaccctccac cggccgcatc ggcgccttca agctgatgaa
     7441 gatcgccggc gcctactggc gcggcgatgc ccggaacgcg cagctccagc ggatctacgg
     7501 caccgccttc gccagccaga aggagctcga cgcccatctg caccagctcg ccgaggcgga
     7561 gcgccgcgac caccggcgcc tcgggcgcga gatgggcctc ttccacatcc aggaggaggc
     7621 aaccggctcg gtcttctggc atcccaaggg ctggcggatc tggcaggagg tcgagagcta
     7681 catgcgccgt cggctcgatg ctgccggcta ccaggaggtg aagaccccgc agctcgtcga
     7741 ccgggtcttg tgggaacgct ccgggcactg ggagaagttt cgcgagcaca tgttcacgct
     7801 cgacgccgac gagcgcgtgc tggcgctcaa gccgatgaac tgcccgtgcc acgtgcagat
     7861 cttcaaccag gccctgaaga gctatcgcga gctgccgctg cgcatgtccg agttcggcgc
     7921 ctgccaccgc aacgagccgt cgggtgcgct ccacggcatc atgcgggtgc gggccttcac
     7981 ccaggacgac gcgcacatct tctgcaccga ggaccaggtc accgccgaga gcgtcgcctt
     8041 ctgcgagctc ctgcggcagg tctatgccga tttcggcttc accgacgtca gcgtgaagtt
     8101 ttccgaccgg ccaaccgtcc gggcgggcga ggacgcgacc tgggatcggg ccgagcaggc
     8161 gctgcgggac gcgaccgaag cggccgggct cgagaccgtg ctcaacccgg gcgaaggcgc
     8221 cttttatggc ccgaagctcg agttcgtgct gcgcgacgcc atcggccgcg actggcagtg
     8281 cggcacgctg caggtcgact tcgtcctgcc cgaacggctg gacgcgacct atgtcggcgc
     8341 cgacggcgcc cggcatcggc cggtcatgct gcaccgcgcc atcctgggct cgatggagcg
     8401 cttcctcggc atcctgatcg agcatcatgc gggccgcctg ccgctctggc tcgcaccggt
     8461 ccagatcgtg gtagcgacgg tcacgaacga tgccgacgcc tacgcccggg aggtcgcggc
     8521 agcgctggag ggtgccggtc tgcgcgtcga ctgcgacacc cgcagcgaca agatctccta
     8581 caaggtgcgc gagcattcgc tcgccaaggt gccgctgatc atggcggtgg gcgggcgcga
     8641 ggcggcggag cactccgtca gcctgcggcg gctgggcagc caggcgcagg aagcgctttc
     8701 gcttgacgcc gcgattgcca tgtgtcagcg agaatccgct gctcccgacc ggcaaacctg
     8761 acgaggcgcc ggtgagcagt ggacggccgg aggccgggcg actcttggtt gtccggtcgt
     8821 ttcggcatat gcgaacgaac gaggatgaaa cattggaggt cgatcacggc tgatggcagc
     8881 aaatcccaaa ggcgacgaac accgcatcaa cgaccgcatc gacgcacgtg aggtccggct
     8941 ggtcgatcaa gacggtggaa tggtgggggt cgtggcgata cgtgacgctc tcacacgcgc
     9001 cgaagacgtg gggctcgatc tggtcgagat ctcacccaac gccacgcctc ccgtctgcaa
     9061 gatcctcgat tacggcaagt tcaagtacga ggcgcagaag aaggccaacg ctgcccgcaa
     9121 gaagcagcgc gtcatcgagg tcaaggagat caagatgcgc ccgtcgatcg atgacaacga
     9181 ctactcgatc aagatgaaga aagtgcgcga gttcctggac gagggcgaca aggtcaaggt
     9241 gacgatgcgc tttcgcgggc gcgagatggc gcaccagcat ctggcgatga acattctcga
     9301 gaaagtgcgc gaggagctgg ccgacctcgc caagatcgag cagtttccga agctcgaggg
     9361 ccgccaaatg ataatggtga tggccccgct caagtgacgg cgccgcaggc tggccggctc
     9421 caggccctgc gcgacgcgct cgccggggcc ggcgtggacg gtttcatctt gcaacgcacc
     9481 gaccagcacg gcagcgagta tctgccggcg gcggagcagc gactcgcctg gctcaccggc
     9541 ttcaccggct cggcgggaca ggcggtcgtc ctgcccgatg ccgctgccgt tttcaccgac
     9601 ggccgctaca ccgtgcagat cggcatcgag gtcgacgcca gcctcttcga acgctgcaat
     9661 tccggtgaca tgccgccgcc gcgctggctg gaggagcggc tgcagccggg catgcggctc
     9721 ggctacgatg ccaagctgat ccgcaaggcg gaacgtgaga agctggcgcg gctctgccag
     9781 ggccgcggtg ccgagctcgt cgccctgacc ccgaacccga tcgatcggat ctggacggac
     9841 cgcccgccgc cgccgctcgc acccgtcact gcccacgacg agagcagggc cggcgaatcg
     9901 agcgtcgcca agcgacagcg catgggcgag gccgtggcca ggcgtggcgc cgactggctg
     9961 ctcgtcaccg ccgccgacag catcgcctgg ctgctgaacg ttcgcggcgg cgatgttccc
    10021 tacaacccgc tctgcctcag ccacctgctg ctcggcgccg acggcacggc gcggtggttc
    10081 gtcgatccgg ccaagctcca gggcggcacc aggctggaca atgccgtcgc caccgagccc
    10141 tacgaggccc tggatggcgc ccttgcggaa ctcgggcgct ccggtgcgcg ggtgctggtc
    10201 gatccgacga tgaatgccgc cggcttcgcc gacatcctgg ctcaggccgg cgcccgggtc
    10261 gtcgagggcc aggacccgat cccgctcgcc aaggcgcgca agaacccgac cgagatcgaa
    10321 ggtgccctgg aggcccagcg ccgcgacggt gcggcggtgg ttcgcttcct cgcctggctg
    10381 gacggtctgg atcacgacca tgccatcgac gaggcggcgg ccgcggaacg gctcgaggcc
    10441 gagcgtgcca aagacccgct ctatcggggc ccgagcttcg agaccatctc ggcgcatggc
    10501 cccaacgccg ccctgccgca ttaccgcgtc accctagaca ccaaccggaa actgacggcc
    10561 ggcaccgttt atctcgtcga ttccggcggc cagtatctcg acggcacgac ggacatcacc
    10621 cgcaccgtcg ccctcggcca gccgacggcc gaggtgcagg agcgcttcac cctcgtcctc
    10681 aaaggccaca tcgccctggc cgaggcggtc tttcccgaag gcacgagcgg cagccagctc
    10741 gacacgctgg cgcggctggc cctctggcgc cgcggcctcg atttcgacca cggcacgggg
    10801 cacggcatcg gcagctatct ctgcgtccac gaggggccgc agcggatcgc caagcgtggc
    10861 ggcgacacgc cgctcgagac cggcatgatc gtctcgaacg agccgggcta ttacgagccc
    10921 ggcgagtacg gtatccgcac cgagaacctc atcctggtcg aggaggcgcc ggccgccgag
    10981 ggcggcgacc gccggctcct gcatttccgc accctcaccc tggcgccgat cgaccgccgg
    11041 ctgatcgacc cgcatcagct caccgagccc gagcgacgtt ggctcgacac ctatcatgcg
    11101 cgggtccggg acgagcttcg gcccctgctc gacgacgtca ccggaaattg gctgaaaggc
    11161 gcaacagcgc cgctatagcc gccgcgccac tgggtcgcgg tgtgtctgct cgcagccgcc
    11221 aatatatgat ggaaccgttg aggcacatca atgcacctcc acggccgcac tgctggatcg
    11281 gtgctggtcg tggtcgtcga cccaccatca gcccggcagg agctcgatga gcgaggcagt
    11341 ttccgtcgag gacgtccagt cccggcaaga gcagcggagc tcgcccctct acaaggggcg
    11401 ggtcgaggtg tacgccaagg ccgtcaaggg ccggttccgg accatcaagt gggccagcct
    11461 cgcgactctg ctcgccatct actacctcgc cccctggatc cgcttcgatc gtggtgcagg
    11521 cgcccccgac caggccatcc tcgccgacat ggccggccgt cgcctctatt tcttctggat
    11581 cgagatctgg ccgcaggagg tctactttct caccggaatc ctggtgctcg ccgccttcgg
    11641 cctgttcttc gccaccgcgc tcttcggccg catctggtgc ggcttcacct gcccgcagac
    11701 cgtctggacc gacctgttca tggcggtcga gcgctggatc gagggcgacc gaaacgagcg
    11761 gatgcgcttc gacaagcagc cctggaccgc cgccaagtgg cgcaagcggg tgaccaagca
    11821 cgccgtctgg ctgctgatcg cgttcctgac cggcggggcc tggatcctct atttcaacga
    11881 tgcgccgacc atgatcggcg agttcttctc gggccgggcc ggagcggccg tctacttctt
    11941 cacgttcctc ttcacggcga cgacctacct gctcgccggc ctcgcccgcg agcaggtctg
    12001 cacctacatg tgcccatggc cgcgcatcca gggcgcgctg gtcgaccagg acaccatggc
    12061 ggtgacctac gaggcctggc gcggcgagcc gcgcggcaag cacaagaagg gccagagctg
    12121 ggatggccgg ggcgattgcg tggactgcaa gcaatgcgtc gccgtctgcc cgatgggcat
    12181 cgacatccgc gatggattcc agctcgaatg catcggctgc ggtctctgca tcgatgcctg
    12241 caacgaggtc atggccaagg tcgagcgccc gcccaacctg atcgcctatg acagtgagcg
    12301 caaccaggag ctcagggccg cgggccagcc cgagcgctat cggatcgtcc ggccgcgcac
    12361 catcctctac accgcgatca tcgccgccgt ggctctgatc atgctgggcg cgctcgcgct
    12421 gcgctcgtcc accgatgtca acgtcatccc cgagcgcaac ccgctcttcg tgacgatggc
    12481 cgacggcagc atccgcaacg gctaccagat caagatcctg aacaaggagc gggcggaccg
    12541 cacctacacc ctggcgctgg ccgacccgat gccgggagcg gtcttcagca caccgaacgg
    12601 gccgagcggc tcatccctcg atctcgccgc cgtctccgac gatgtcgcca cccaccgtgt
    12661 cttcctggga ttgccacgcg cggcattgac cgaagccgcg acaccgatca cgctggttct
    12721 gaccgacaac ctcaccggcg aggagcgccg cttctcgacg gtcttccgag gacccgatac
    12781 atgagcgtcg gcctgtccgg cccggccacg cggcatcgcc cgggctggta cattccctgg
    12841 atcctggtcg ccggcttcgg cgtcgttctc gccgtcaacc tggccatgat ctggatcgcc
    12901 atgagcacct ggaccggcat ctcgaccaac cgcgcctacg acaagggcat cacctacaac
    12961 cgcaatctcg atgccgcgga gcgtcaggcg gcgctgggct ggcgggccag catcgtgacc
    13021 gcgcaggcgg ccggactcgc cggcacgatc tcggtccacc tggtcgacgc ccggggccag
    13081 ccactcgagc gcatcgacgt ccaggcggac ttcgagcggc cgacacatca gggctatgat
    13141 ttcgccctga ccctggagcg caccggcccc ggcacctatg cggcacggtt cacggccccc
    13201 ctggctggcc tctgggacgt tcgggtgatc gccacgcgcg gcggcgaccg gctggtcgtc
    13261 accgagcgcc tgatgctgca ctgaagtcat ggtcgcgcac gcggcgcatt cctccggtcg
    13321 atcgggcgct gcagacgaca gtgcggagaa accgaccgac gtcaccggct tcgtcaggat
    13381 cgacccggcg agcggcaccg ccatcctgca gctcatggtc gagggtgtcc attgcggcgc
    13441 ctgcgtgcgg cggatcgagc gggcgctcga ggccgagccc gacgttcaaa ccggccgcct
    13501 gaacctgacc acgcgacggt tgaccctcgc ctgggagggg ccgccggcac gtggcaacga
    13561 gctggtcgcg aaaatcgagg gcctcggcta tccggtcgtc ccgttcgacc cggaaagcct
    13621 cgccgtctcc gacaaccggg cggagcgcga cctgctccgc tgcatggcgg tcgccggctt
    13681 cgccgccagc aatgtcatgc tgctcgcggt ttccgtctgg gccggtcatt tctccgggat
    13741 gggggacgcg acgcgcgact tcctccactg gatgcaggcg ctgatcgtcc tgccgtcgct
    13801 cgcctttgcc ggccgcccct tcttccgttc ggccatggcg gcactccgtg ccggccacgc
    13861 caacatggac gtgccgatct cgctggccct cgtcctcgcc ccggcgatga gcctgctcga
    13921 gacgatacag ggcggcgagc acgcctattt cgacagcgcc atcatgctgc tcttcttcct
    13981 gctgatcggt cgctacctcg acagccgggc gcgtggcgtc gcccgctccg ccgtcgagcg
    14041 actgatgacg cttcgcgcca ccgccgtcac cctgctcctg ccagacggcc agacgcgcag
    14101 ctgccggccg gagcagctcg aggccggcca gaccgtcctt gtcgccgcag gcgagcgcat
    14161 cggtatcgac ggggccgtcc gagccggcca gagcgagatc gacacgagcg ccatcactgg
    14221 cgagaccctg ccggccatcg ctacccccgg cacgcgcgtc ttcgccggca cgatcaatct
    14281 ggcggctccc atcgaggtga cggtccgctc ggtcggcgag ggcacgctgc tcgccgagat
    14341 cgtcaagctg atggagctcg ccgagcagaa gcgcggtcgc ttcgtcgccc tggccgaccg
    14401 tatcgcccgc gcctactcgc cggtcgtcca tctgcttgcc gccctcacct tcctcggctg
    14461 gtggctggcc atggatgccc cctggcaggt ggctctgctc aatgccgtcg ccgtcctgat
    14521 catcacctgc ccctgcgcgc tcggcctcgc cgtgccggcg gtgcaggtgc tggccagcgg
    14581 ccggctgatg cggcggggcg tcctgctcaa gtccgcgacc gcgctcgagc gcctcgccga
    14641 ggtcgacacc gttgtcttcg acaagaccgg cacgctgacg gagggcgacc tcgtcctggt
    14701 cgaggggaac ggcgagcagg acctcctggt tcaggccgct tccatggcca ggagctcgcg
    14761 tcatccgctc gcccgcgccc tcgcccgcgc ctgtcccggg gcgcgcgccg ccgccgctgt
    14821 cgaggagctg ccggggctcg gcctcaggct caccacggag gccggcgagc ttcgcctcgg
    14881 tcgccgcagc tgggcagcgc ccgggattgc cgacgacgcc gatgccggca ccgagctctg
    14941 gctggcgctg ccggggcggc cgccgcatcg ctttgccttc cgggaccgcc cgcgcaccga
    15001 tgccgctgcg accgtcaggg ccctccaggc acgcggtctc gaggttcagc tcctctccgg
    15061 cgatcggccg cggcctgtgg cggcgctggc cgccgagctc ggcatcgcga cctggcaggc
    15121 cggctgccgg ccggacgaga aggtggcgca cctggccgct ctcgctgcgg cggggcgcaa
    15181 ggtgatgatg gtgggagacg ggctcaacga cgcaccggcg ctggccgcgg cgcatgtctc
    15241 gctgtcgcca tcgacggcgg tcgacatcag ccagacagcg gccgacgccg tcttccaggg
    15301 gcggttcctg gcgccggtcg tgctcgtcct cgagatcgcc gggcggagcc gccggctggt
    15361 cgagcagaac ctcggactcg ccttcggcta caatgtcctg gcggtgccgc tcgccgtgct
    15421 cggcttcgtc acgccgctca tcgcggcgat ctgcatgtcg gcgagctcgc tcgtcgtcgt
    15481 cggcaattca ctccgcctga atcggaaata gcagagcggc cgtgggcgcc gccgggctca
    15541 ggcgtcgtgc gtgcggcgca ggacccgctc gggtgtggtc gcctgatgca cgaagacgac
    15601 gatgatggcg atgccgaaga ggaagatcat gacaccgaag aagccgaaca tcgtgtcggc
    15661 cgcccggctc atgatgatca ggccgaggag ggcgagcacg cccatcgcaa tgcccccgag
    15721 ccaacgcccc agatcgttca ttccgttatt cctcgcaagt caggtctaac gctctcaggc
    15781 ggcgtccaac tcgacgatgt cgcggtaggc atgccaggtc gcatggccga tcagcggcag
    15841 cgtcacaatc agtccgagat agagcgtgac cagcccggcc atcatgaaga tcacgatcag
    15901 cccgccccag aagagcagcg gcatcgggtt ggcctgcacc gcccgcacgc tcgtcgcgat
    15961 cgcctcgatg atattggcgg cgggccggtc gagaagcagc gggatggaga cgacgctgat
    16021 cgagaaggcg acgagcgcca gcacgccacc gacggccgag ccgatgatca agaagggcca
    16081 gctgtcggcc gacaggaacg tcgcgacgaa gagattgtgg aagtccggcg gctccatgcc
    16141 gaaatagagg aagaagagca atgccgcggt ccgcgcccag aagaacatga ggagcaggag
    16201 cgccatgccc atgagcccca tctggctcgg gttgcggttg aaggcgccga gcgcatcggc
    16261 caggctggtc ggcaggccct gctcgctccg gcgactggct tcgtagaggc cgaccgccag
    16321 gatcgggccg acgatcagga aaccggccat gatcggcaat accagatagg gcatgtcgaa
    16381 ggcgaacagc ccccaggtga tcaggtagcc cgtcaccgcc gcgagcgtgc catagacgaa
    16441 gctcacgccg ggtgtcttca ggcagtcctg ccagcccttg cgcagccaag tccacggctg
    16501 gtccatctcc agcttgcgaa ctctgaccgt cggctgcgcg aacaccggaa cggcgtcggc
    16561 catcgtcgtc actcccagat cattcccgcc accgggctgg cccggatcga aggcaacctt
    16621 gcacagcccc cactttacac aaggccaacg cggccggcca ccaccgccga tgtgtcacac
    16681 ctcgtgtggc gcaatcgggg ttgcgcaaac aggggcttcg tagtgcaaag gtgatcgccg
    16741 gctcttgact tggatcaatg tcaggtcgct gctgcgcagg caatcccttc agcgcagcac
    16801 gggccgcatt ttccaccaac ggcacgtggg acgcggcaag cgggagaacg agatggcagc
    16861 ggtgatgacg gccccggttg gccgtgccct gagcgcctac gacgaggacg tggtccggct
    16921 gttcacgctg gcgacggtct tctggggcgt cgtcggcttc ctcgtaggcg tcgtcatcgc
    16981 cttccagctc gcctatcccg tgctcaacct cgatctcgag tggacgagct tcgggcgcct
    17041 gcggccgctg cacacctctg cggtagtctt cgccttcggc ggcaacgcgt tgctcggcac
    17101 ctcgctctac gtggtccagc gcacctgtcg gaccagcctg ttcggcgggc cgctgctcgc
    17161 cagcttcctg ttctggggct accagctctt catcgtgctc gccgccaccg gctacgtcat
    17221 gggcgtcacc cagagccgcg agtacgccga gccggaatgg tatgtcgacc tctggctgac
    17281 cgtggtctgg gtcacctacc tcgtcatctt catgggcacg ctgctcaagc gcaaagagcc
    17341 gcacatctac gtcgccaact ggttctacct cgccttcatc atcaccatcg cgatgctgca
    17401 catcgtcaac aacctggcgg tgccggtgac gttcttcggg atcaagagct actcgctctt
    17461 cgctggcgtc caggacgcgc tgacacagtg gtggtacggc cacaacgccg tcggcttctt
    17521 cctcaccgcc gggtttctcg gcatgatgta ttatttcgtg ccgaagcagg cgaaccggcc
    17581 ggtctattcc taccgcctct cgatcatcca cttctgggcg ctgattttcc tctatatctg
    17641 ggctggcccg caccatctgc actacaccgc gctgcccgac tgggc
//
LOCUS       DAOTFI010000097        17664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3854_length_17664_cov_7.157192, whole genome shotgun sequence.
ACCESSION   DAOTFI010000097 DAOTFI010000000
VERSION     DAOTFI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17664
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3854_length_17664_cov_7.157192"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="P5558_11460"
     CDS             <1..1282
                     /locus_tag="P5558_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRY24973.1"
                     /translation="VEVFAGDDDPYFRARAADLLDLRDRVMAALAGGGGPAMAPPAGA
                     IVTARDLSPSRFLGLDWAVLGGAALGAGSAASHVAMLARARGIPLVTALGPIEPVAAT
                     AVLDARDGFLVVGPKPATERRYRGRLAVDREEARAAAKVVAEPARTASGRPISVMVNV
                     DDPDAVPDDLLRAGDGVGLLRTEFLFIGRAERPDEETQYRAYARLLQRLGGRPLILRT
                     LDIGGDKPLPDLDIPAETNPFLGLRGLRLCLDRPELFRPQVRAALRAAADGPLSIMLP
                     MVSRAAEVLEARSFIEAEARALDLAVPPVGIMVETPAAALALDTMPVDFASIGSNDLT
                     QYVMAASRDAGGRVAELIDPLDPAVLRLIRMVVEMAAAKGLPLSLCGDMASDRAGIEA
                     LVAIGIERVSVAPAALGRVKLWIAGCAGRAHGLP"
     gene            complement(1323..3140)
                     /locus_tag="P5558_11465"
     CDS             complement(1323..3140)
                     /locus_tag="P5558_11465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRY24974.1"
                     /translation="MTGHAEPAARISAVQEDIVRLRLTDPARGRLVKNEVVYIRPARA
                     PEQRLKAEILRVMGASADAQVYESTRGVGLGDPVEQTGNLLSIRLGPGMLGQVYDGLQ
                     NPLEALAVGHGLFLPKGVAAPGLDPASKWSFTSTVRIGDRVRGGDPLGAVPEGPVSHK
                     IMVPFDLDETFEVTWLREGSVTVDEPIARLEAASGAERTLTMAQDWPVRTPLPRRLLA
                     RGWSTRLFPDRPMITTQRIIDTFFPIAQGGAACIPGPFGAGKTVLLNLIARHADVDVV
                     VLVACGERAGEVVETITEFPRLKDPRTGGSLMDRTVIVCNTSSMPVAAREASIFTGIA
                     IGEYYRQMGLRVLMIADSTSRWAQAMRETSGRLEEIPGEEAFPAYLDSAIKGVYERAG
                     AIRANDGAEGALTIIGSVSPAGGNFEEPVTQSTLAAVKCFLGLSYDRAYKRFYPAIDP
                     LLSWSRYLRQLEGWSAEHLAPDWVEQVEAMTALLQRGDEIARMEQVTGEEGISLEDFV
                     IGRAAAFIDMVFLQQDAFDDIDASAPPARQKALFDRVHAIATRGYGFADKDAARRHFT
                     RLTGLFKNLNYAAPGSPDAARLEAEIEALDAEAPVLEQA"
     gene            complement(3137..3826)
                     /locus_tag="P5558_11470"
     CDS             complement(3137..3826)
                     /locus_tag="P5558_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24975.1"
                     /translation="MIWVDQPYVTLMASLPAIRFLTEKEPPINRQRLMERLKDLPPAD
                     QETLRELTEVVSWRFVDTTQDDAAFLARAAALLERIASPTLAAAVRQRLELRTVIAAL
                     RRRHAGEDAPPKGVRWGFGPHLERIRASWSLPDLGVAAFYPWLPAAKEALDAGDTVAL
                     ERLLLETAWQQQARHAEGHEFDFEAVALYMLRWSMADRWARYDADEARRRFAELLDAA
                     LADAPEIPEAA"
     gene            complement(3823..4515)
                     /locus_tag="P5558_11475"
     CDS             complement(3823..4515)
                     /locus_tag="P5558_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24976.1"
                     /translation="MSRGSAAEQTASGIETLIARLRDEGVSEGRAEAERLIAEANARA
                     RAIMEAAEAEAAAKLAAARREIDAHRRAGQDALQAAARDTVLDLKEQLVRKLATDVEK
                     TVGDAFRDESMLERMILQVVGRAREEAKVESSGEVRVLLPRSAVGLDELRRRPEELRE
                     GSLSHFVAGIAADMLREGVTFDRAEDAAEGLRIVLEDRGITLDLSDAAVASALMVHLQ
                     PRFRALLEGLVK"
     gene            complement(4526..4969)
                     /locus_tag="P5558_11480"
     CDS             complement(4526..4969)
                     /locus_tag="P5558_11480"
                     /inference="COORDINATES: protein motif:HMM:NF007200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRY24977.1"
                     /translation="MSDLIVLLGWLGIYAPVALGALGSAIGVAIAGQAAIGAMLETDG
                     GYGRFVGAAAIPSSNVIYGIVVMFALQQPVTVQNGGALFSIGGLLGIALLYTGVWQGR
                     ALASAIAASKSKTEIFGLALAPAAIVEGFAVFAFVFALVLAGGIA"
     gene            complement(4966..6756)
                     /locus_tag="P5558_11485"
     CDS             complement(4966..6756)
                     /locus_tag="P5558_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24978.1"
                     /translation="MTIAPMLKVTLCGLAHEKAPVLEELQALGALHLIPLRRPGPLTP
                     DDPAARRRADTAFHHLQDAPEQLTPWPLGKPFDADAVIGEIIENRLRLRRLADRSFEL
                     DQMIEALGPWGDFRLPQPAELRGEQLWLYALPRKDRAALEQVQLPWAIVGRCETALHV
                     AVVAKQPPPRSLLPVPRLETGDATLAELRAERAEVGIALEMAERARADLTRWRLALGA
                     KLAAAEDADDRRAAAHQTLDDDRVFAVQGWAPADTAPALRRLAEERGLALVAEPPGRD
                     DTPPTLLRAEGERKAMGSDLTSFYMSPGYRSWDPSLVVFTSFAIFFAMILADAGYALL
                     LGLLTAFYWRRLGSSASGRRFRVLLAALGGASFVYGVAAGSYFGFAPPAGGWLERLRI
                     VDVTDFEAMMRLSILIGAAHITIALAALAWLERGRGEGLSALGWIAVIAGGLGLWLGE
                     GEAVPRLATLLLGGGLVAVFAGGLTAPPAGATLAARLGGAALGLTRATKLFGDLLSYL
                     RLFALGLASASLAVTFNGLALGILHDRPGIGVLLAILVLLFGHAINIAIGLMSGVVHG
                     LRLNYIEFFGWGLPEEGYPFRAFAKREKTA"
     gene            complement(6753..7373)
                     /locus_tag="P5558_11490"
     CDS             complement(6753..7373)
                     /locus_tag="P5558_11490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRY24979.1"
                     /translation="MAQVQLSKSSLTREKENLALYQRYLPALDLKRQQLMGERQRARR
                     EIERLGLEVARVIEAAGEAIPMMADRRVSLDGLVRLGEVRLGTQNVAGVKLPTVEHVE
                     LIRSAYGRLVRPHWVDHVADRLAAAMRLRVEVEVAEKRLAGLERAVARITQRVNLFDK
                     VLIPQTRTNIRRINVYLGDAERAAVAGAKIAKRKREAEAARPEAGA"
     gene            complement(7373..8719)
                     /locus_tag="P5558_11495"
     CDS             complement(7373..8719)
                     /locus_tag="P5558_11495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRY24980.1"
                     /translation="MADIAITYDRVLSIIGGVIRIALPKETADDPEGPRFGDLAVVSE
                     GDTLPRLAQVVRVGDGEASLQVFAGVEGLSTRSRVRFLGHAPKAVFSPSVLGRVFAGD
                     GRVLDRGPSLDHEPKVEVDGPSANPARRAMASGMIRTDVPMIDVFNSLVESQKIPIFT
                     SAGEPHNALLARIGIQAEADVVVFGGLGLTFDDYWTFRTTFEDAGVFMRTVMFVNQAS
                     DPALERLLIPDMALAVAERFAVEEKKRVLVLLTDMTAYADALKEVGISLDRVPSTRGY
                     LGDLYSQLARRYEKAAVFPGGGSVTILSVTSMPGGDVTHPVPDNTGYITEGQFYLRNG
                     RIDPFGSLSRLKQHVIGKETREDHSAVMNTMIRLYSGARDAQRKQAMAFELSDLDQRL
                     LRYGSFFESRFMDLDVALPIEQALDLGWQTMAACFAPEELLVRQNLIDKYYPRSAA"
     gene            complement(8755..10131)
                     /locus_tag="P5558_11500"
     CDS             complement(8755..10131)
                     /locus_tag="P5558_11500"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRY24981.1"
                     /translation="MAISVFDIFKIGVGPSSSHTFGPMVAASRFLGELEEAGLGDAVG
                     GVEVELYGSMALTGKGHYTDLASMLGLEGADPETLDSAGIPARRERIERERTLHLGGR
                     RMVPFDPDKHIHWRGDKTYPKHPNGIAFAATDGAGQVLHRSLYFSIGGGFIVGDSDSI
                     HPTAAIGDNSKPFPYTTGAELAALCAREGKTIAQIVFENELVWRTEKAVRDRLLEIAA
                     VMEKSIEVGLQGEGEVPGGLGVKRIAKDIMSQVKEGTRGGHLFELSDHINAYALAVME
                     LNASLGQVVTAPTMGSAGIVPAVLKAYKQYAPGASDDGVCDFLLAAGGCSIPVKINAS
                     FSGAEVGCQGEVGAASLMAATGLTQALGGSVTQVLNAGAMALEHNLGLTCDPVKGLVV
                     VPCINRNPLAANKAVNAVRLTMLRGDEPARPSFDEVIKALYLTGKDMSDKYKETSQGG
                     LAVVWVDC"
     gene            complement(10170..11018)
                     /gene="pflA"
                     /locus_tag="P5558_11505"
     CDS             complement(10170..11018)
                     /gene="pflA"
                     /locus_tag="P5558_11505"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRY24982.1"
                     /translation="MSELIGSRYELRRTRTGAVEAKGRDYAEVFPEDLEAEADEEVGG
                     YVHSIEVGSTVDGPGIRFVLFLTGCPLRCQYCHNPDTWHKRNGKRYTLGRITREIGKY
                     AKVLKLSKGGLTVSGGEPTMQPRFLRRVMQATKAMGVHTCLDTSGRLGRNLTDADLAD
                     IDLQLLDIKSGDPATYERVTGHPLQPTLDYARRLSDLGRPLWVRFVLVPGLTDDPDNV
                     ARVADICAGLEPLERVEILRFHQMGKAKYETLGIPYPLADAEPPSPELTERVRAQFRE
                     RGLTVY"
     gene            complement(11023..13278)
                     /gene="pflB"
                     /locus_tag="P5558_11510"
     CDS             complement(11023..13278)
                     /gene="pflB"
                     /locus_tag="P5558_11510"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HRY24983.1"
                     /translation="MTFHHRPERPGFAEAWRGFEPGLWCDRVDTRDFIQRNVEPYLGD
                     AGFLAGATPRTQKVWEALTPLLAEERRKGVLDVSQEPSSIIAHGPGYIDKELELIVGL
                     QTDAPLKRAIMPSGGWRVVADSLEAYGFEPDPAVARIFTTYRKTHNDGVFSAYTPEIL
                     KCRRSGVITGLPDAYGRGRIIGDYRRLALYGLDFLIEDKEREKAELDSRHATEEIIRL
                     REELTEQLGSLRQIRVMAERYGYDVAGPARTGREAVQWLYFAFLAAIKEQNGAAMSVG
                     RITTFLDIYLERDIREGTLDEAGAQELIDDLVIKFRIVRFLRTPAYNELFSGDPTWVT
                     DYIGGMGDDGRALVTRTSFRILQTLYNLGPAPEPNLTVGWSTRLPDGFKSFAARCSID
                     TSSIQYMNDDLMRPKWGDDASIACCVSGMATGKQMQFFGARVNLLKALLFAINGGRDE
                     ISGAQVGPPLAPITADVLDYDEVMARFDVMAEWLARTYVNALNIIHYMHDKYCYEAAL
                     MAFHDRDVLRTMACGLAGLSHTADSLSAMKYARVRAIRDDRGLVVDYAVEGEFPRYGN
                     NSDRADAIAVMLTRMLMDKIRNHPTYRDALHTQSILTITSNVVYGKQTGNSPDGRRSG
                     EPFAPGANPSNGADTRGALAAMMSVAKLPYDDALDGISLTISVVPTSLGSAEERIAKA
                     VGGLDAYFGSNGFHVNLNVLNRDTLREAMERPEDFPQLTIRVSGYAVNFVKLTKEQQL
                     DVLNRTFHGKF"
     gene            complement(13301..14242)
                     /locus_tag="P5558_11515"
                     /pseudo
     CDS             complement(13301..14242)
                     /locus_tag="P5558_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
     gene            14520..16337
                     /locus_tag="P5558_11520"
     CDS             14520..16337
                     /locus_tag="P5558_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRY24984.1"
                     /translation="MSDAAIVALIIPAALALFIWNRVPAVIVGVGVSLALFATGVITA
                     SQALAGLGDPTVILIAGLFVVAAGLEAAGVTTWAGQKLVETAGGSKSLALLLVMLMAA
                     IFCAIISVNATVAALLPVAVIVAIRLGVPTSQLLLPMCFGAHAGTMLTLIGAPLNVIA
                     YDAARDAGYGGIGFFEYAVAGLPIMIGSLAIMYLTQRFLLPHRDGLNLPADFSSHAAT
                     LVEQYRLEDGLHRLRLRETSPLVGQPRSAVDLARYPGLAFVAIEDASGGVSTRDSLAA
                     GDSLLVRGDADWAGRLAGDLGLAIREAAGDAAQTLFNRGSGLAEVVIPPRSGFIGETA
                     FPGMATPSGDLMILAIQRGGEDLGIEPVTIAAGDTLLLQGTWAAFDQRLADPRVLAVH
                     SPELVRRQAIPLGHRAWQALGVLLLLVVLLVTAPFPSAITAVICAGLMVVLGVLTVPQ
                     MWRGVDWNTCILVGGMIPLAGAMTQSGLAQDIADGLILLIGWAGPLAVLAGLFAVTAI
                     LTQVMSNTATALVMLPVAVASGPEMGVSAMPMIIAVTVGAHAALLTPMATPVNLMVVG
                     PGGYTFGDYWKYGLPILLWWLVVCVLIVPLWWRFDAAPL"
     gene            16495..17607
                     /locus_tag="P5558_11525"
     CDS             16495..17607
                     /locus_tag="P5558_11525"
                     /inference="COORDINATES: protein motif:HMM:NF025878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY24985.1"
                     /translation="MSDRRAPATGLLPEARQTAAGARNDPGQQDGRARPVISFATDTF
                     PAELAFEAWREQFGQLNEVIVARGAGFSGRSRFWQLGDFTVAVNETSAMRLVRSAAHL
                     RRDDLDHWLLRVSAEGVVRSRSGERAFRSAPGELVVERLNEAYEDEWSADRWVMLVVP
                     PGTCPEIEAGLAAMPRGPVGGVEAALLADFLLSLARRLPEALESDLPALAHTTQTMIA
                     ATFAGRHGAARESPLAARLRVERTIRASLASARLDPSRIAALAGVSRATLYRLLEDQG
                     GVAAYVRRLRLEAVHAALCNLGQAHVPIARLAERAGFHSPAAFNRTFRAAFGASPGEV
                     RERAARQDPPLPRAPDSSPFHRGFLDLAFPAGAPAH"
BASE COUNT         2427 a         6128 c         6445 g         2664 t
ORIGIN      
        1 ggtcgaggtc ttcgccggcg acgacgaccc ctatttccgc gcccgggccg ccgatctcct
       61 ggacctgcgc gatcgcgtga tggcggcgct cgccggcggc ggcggcccgg cgatggcccc
      121 gccggcgggc gccatcgtca ccgcccgcga cctgagcccg tcccgcttcc tcggtctcga
      181 ctgggcggtg ctgggtggtg cggcgctcgg ggcgggcagt gccgcgagcc acgtcgccat
      241 gctcgcgcgt gcccggggca taccgctggt gacggcgctc gggccgatcg agcccgtggc
      301 ggcgacggcc gtgctcgatg ccagggatgg ctttctggtc gtcggtccga agccggcgac
      361 cgagcgccgc taccgcgggc ggctcgcggt cgaccgcgag gaggcacggg ccgcggcaaa
      421 ggtggtggcg gaacccgcgc gcaccgcatc cggccggccg atctcggtga tggtcaatgt
      481 cgacgacccg gatgccgtgc cggacgatct cctgcgcgct ggcgacggcg tcgggctgct
      541 gcgcaccgag ttcctgttca tcggccgagc ggagcggccg gacgaggaga cgcagtatcg
      601 ggcctatgcc cgcctgctgc agcgcctggg cggccggccg ctgatcctgc gcaccctcga
      661 catcggcggc gacaagccct taccggatct cgatattccg gccgagacaa acccctttct
      721 cggcctgcgg ggcttgcgac tctgcctcga ccggccggag ctcttccgcc cgcaggtcag
      781 ggcagcgctt cgggcggcgg cggatggccc gctctcgatc atgctgccga tggtcagccg
      841 cgccgccgag gtcctggaag cgcggtcctt catcgaagcc gaggcgcgcg ccctggacct
      901 agccgtgccg ccggtcggca tcatggtgga gaccccggca gccgccctcg ccctcgacac
      961 catgcccgtc gatttcgcct cgatcggctc gaacgacctg acgcagtacg tgatggcggc
     1021 ctcgcgcgac gccggcggcc gcgtcgccga gctgatcgac ccgctcgacc cggccgtgct
     1081 ccgcctcatc cggatggtgg tggagatggc ggcggccaag gggctgccgc tctcgctctg
     1141 cggcgacatg gcgagcgacc gggcgggcat cgaggcgctc gtcgccatcg ggatcgagcg
     1201 cgtttcggtg gcaccggcgg cgctcggccg ggtcaagctg tggattgccg gctgcgccgg
     1261 ccgggctcac gggctgccgt gagagcaccc gagcgcctcc gggccgcgat cgctgctgcg
     1321 cctcaggcct gctcgagcac cggggcctcg gcatccaagg cctcgatctc ggcctcgagc
     1381 cgtgctgcat cgggggatcc gggtgccgcg tagttcaggt tcttgaagag gccggtgagc
     1441 ctggtgaagt ggcggcgggc cgcatccttg tcggcgaagc cgtagccgcg ggtggcgatc
     1501 gcgtggacgc gatcgaacag cgccttctgc cgggcgggcg gggcgctggc gtcgatgtcg
     1561 tcgaaggcgt cctgctggag gaagaccatg tcgatgaagg cggcggcccg gccgatgacg
     1621 aagtcctcga ggctgatgcc ctcctcgccg gtcacctgct ccatccgggc gatctcgtcg
     1681 ccgcgctgca gcagggcggt catcgcctcg acctgctcaa cccagtcggg ggcgagatgc
     1741 tcggccgacc agccctcgag ctggcgcaga tagcgcgacc aggagagcag cgggtcgatc
     1801 gccgggtaga accgcttgta ggcgcggtcg taggagaggc cgaggaagca cttgacggcg
     1861 gcgagcgtgg attgcgtcac cggctcctcg aaattgccgc cggcgggcga gaccgagccg
     1921 atgatggtca gggccccttc ggccccgtca ttggcgcgga tggcaccggc gcgctcgtag
     1981 acccccttga tcgcgctgtc gagataggcc gggaacgcct cctcgcccgg gatctcctcg
     2041 agcctgcccg aggtctcgcg catcgcctgg gcccagcgcg aggtcgaatc ggcgatcatc
     2101 aggacgcgca ggcccatctg gcggtagtac tcgccgatcg cgatgccggt gaagatcgag
     2161 gcctcgcgcg cggccaccgg catcgacgag gtgttgcaga cgatgacggt gcggtccatc
     2221 agcgagccgc cggtgcgcgg gtccttgagc cgcgggaact cggtgatggt ctcgaccacc
     2281 tcgccggcgc gctcgccgca ggcgacgagc accacgacat cgacatcggc gtgccgggcg
     2341 atcaggttca agagcaccgt cttgccggcg ccgaacggcc ccgggatgca ggcagcgccg
     2401 ccctgcgcga tcgggaagaa cgtgtcgatg atccgctgcg tggtgatcat cggccggtcg
     2461 gggaagagcc gggtcgacca gccgcgggcg agcagccggc gcggcagggg ggtccggacc
     2521 ggccagtcct gcgccatggt gagcgtgcgc tcggcacccg aggccgcttc gagcctcgcg
     2581 atcggctcgt cgaccgtgac cgaaccctcg cgcagccagg tgacctcgaa ggtctcgtcg
     2641 agatcgaagg gcaccatgat cttgtggctg accggccctt ctggcacggc gccgagcggg
     2701 tcgccgccgc gcaccctgtc gccgatgcgc acggtggagg tgaaggacca cttgctggcc
     2761 ggatcgaggc cgggcgcggc gacccccttg gggaggaaga ggccgtgacc gacggccagg
     2821 gcctcgagcg ggttctgcag cccgtcatag acctggccca gcatgccggg gccgaggcgg
     2881 atcgagagca ggttgccggt ctgctcgacc gggtcgccga ggcccacgcc gcgggtgctc
     2941 tcgtagacct gggcatcggc gctggccccc atgacgcgca ggatctcggc cttgaggcgc
     3001 tgctcggggg cgcgggcggg gcggatgtag accacctcgt tcttgaccag ccggcccctt
     3061 gcggggtcgg tcaggcgcag ccggacgatg tcctcctgca cggccgagat gcgggcggcg
     3121 ggttcggcat gaccggtcat gcggcctccg ggatctcggg tgcgtcggcg agcgcggcat
     3181 cgagcagctc ggcgaagcgg cgcctcgcct cgtccgcatc gtagcgcgcc cagcggtcgg
     3241 ccatcgacca gcgcagcatg taaagcgcca cggcctcgaa gtcgaactcg tggccctcgg
     3301 catggcgcgc ctgctgctgc caggcggtct ccagcagcag gcgctcgagc gccacggtgt
     3361 cgccggcatc gagcgcttcc ttggcggccg gcagccaggg atagaaggcg gcgacaccga
     3421 ggtccggcag cgaccagctc gcccggatgc gctcgagatg cggcccgaag ccccagcgca
     3481 cgcccttggg cggcgcatcc tcgccggcat ggcgacggcg cagcgcggcg atgaccgtgc
     3541 gcagctcgag ccgctggcgc acggcggccg cgagggtcgg gctggcgatc cgctcgagca
     3601 gcgctgccgc ccgcgcgagg aacgcggcat cgtcctgggt ggtgtcgacg aaccgccagg
     3661 agaccacctc ggtgagctcg cgcagggttt cctggtcggc cggcggcagg tccttcaacc
     3721 gctccatcag gcgctggcgg ttgatgggcg gctccttctc ggtgaggaag cggatcgcgg
     3781 gcaggctcgc catcaaggtg acgtagggct gatcgaccca gatcatttga cgagcccttc
     3841 gagcagcgcg cgaaagcgcg gctggaggtg aaccatgagc gcgctcgcca cggcggcgtc
     3901 gctgagatcc agcgtgatgc cgcgatcctc gagcacgatg cgcagcccct cggcggcatc
     3961 ctcggcacgg tcgaaggtga cgccttcgcg cagcatgtcg gcggcgatgc cggcgacgaa
     4021 atggctgagc gagccctcgc ggagctcctc cggccggcgg cgcagctcgt cgagccccac
     4081 tgccgagcgt ggcaggagga cgcggacctc gccggagctc tcgaccttgg cctcctcgcg
     4141 ggcccggccg accacctgga ggatcatccg ctcgagcatc gactcgtcgc ggaaggcgtc
     4201 gccgacggtc ttctcgacgt cggtggcgag cttgcggacg agctgttcct tgaggtcgag
     4261 cacggtgtcg cgcgcagcgg cctgcagcgc gtcctgcccg gcgcggcggt gggcgtcgat
     4321 ctcgcggcgg gccgccgcca gcttcgccgc ggcctccgcc tcggcggcct ccatgatcgc
     4381 gcgggcacgg gcattggcct cggcgatcag ccgctccgcc tccgcccggc cttcgctcac
     4441 cccctcgtca cgcagcctgg cgatcagggt ctcgatgccg gacgcggtct gctccgcggc
     4501 gctgcctctg ctcatcgccc gggcctcacg cgatgccgcc ggcgagaacc agcgcgaaga
     4561 cgaaggcgaa gaccgcgaag ccctcgacga tggcggcggg cgccagcgcc aggccgaaga
     4621 tctccgtctt gctcttggag gcggcgatgg cgctcgccag cgcccggccc tgccacacgc
     4681 cggtatagag cagggcgatg ccgagcaggc cgccgatgct gaagagggcg ccgccgttct
     4741 gcactgtcac cggctgctgc agcgcgaaca tgacgacgat gccgtagatc acgttcgagg
     4801 acggtatggc cgccgcgccg acgaagcggc cgtagccgcc gtcggtctcg agcatcgccc
     4861 cgatggcggc ctgcccggcg atcgcgacac cgatcgcgct gcccagggcg cccagggcca
     4921 cgggtgcgta gatcccgagc cagcccaaaa ggacgataag gtcgctcatg ccgtcttctc
     4981 cctcttggcg aacgcccgga acgggtagcc ctcctcgggc agcccccagc cgaagaactc
     5041 gatgtagttg agcctgagcc cgtgcacgac gccgctcatc aggccgatgg cgatgttgat
     5101 ggcgtgaccg aagagcagca cgaggatggc gagcagaacg ccaatgcccg gccggtcgtg
     5161 caggatgccg agggcgagcc cgttgaaggt gacggcgagc gaggcgctgg cgaggccgag
     5221 cgcgaagagc ctgagatagc tcagaaggtc gccgaagagc ttggtcgccc gggtgaggcc
     5281 cagcgcggcg ccaccgagcc gggcggcgag cgtggcgccg gcgggcgggg cggtgagccc
     5341 gccggcgaag accgcgacga gcccgccgcc caggagcagc gtggcgagcc gaggcacggc
     5401 ctcgccctcg ccgagccaga gcccgaggcc gcccgcgatg acggcgatcc agccgagcgc
     5461 cgacagcccc tcgccgcgcc ctcgctcgag ccaggcgagg gcggcgaggg cgatggtgat
     5521 gtgggcggcg ccgatcagga tcgagagccg catcatcgcc tcgaaatcgg tgacgtcgac
     5581 gatccggagc cgctcgagcc agccgccggc gggcggcgcg aagccgaaat acgagccggc
     5641 agccacgccg tagacgaagg aggcgccgcc cagcgccgcc agcaggacgc ggaaccgcct
     5701 gcccgatgcg ctcgacccga ggcgccgcca gtagaaggcc gtcagcagcc cgagcaggag
     5761 ggcgtagccg gcatcggcca ggatcatcgc gaagaagatc gcgaaggagg tgaagacgac
     5821 gaggctcggg tcccagctcc ggtagccggg gctcatgtag aagctggtga ggtcgctgcc
     5881 catggccttg cgctcgccct cggcgcggag cagcgtgggt ggcgtgtcgt cgcggccggg
     5941 cggctccgcg accagggcga ggccccgctc ctcggcgagc cggcgcagcg ccggggccgt
     6001 gtcggccggc gcccagccct gcacggcgaa cacccggtcg tcgtcgagcg tctggtgggc
     6061 ggccgcccgt cggtcgtcgg cgtcctcggc ggcggccagc ttggcgccca gggcgagccg
     6121 ccagcgggtg agatcggcgc gggcgcgctc ggccatctcg agggcgatgc cgacctccgc
     6181 ccgctcggcc cgaagctcgg ccagcgtggc atcgccggtc tcgagccgcg gcaccggcag
     6241 gaggctgcgc ggcggcggct gcttggcgac gaccgcgacg tgcagggccg tctcgcagcg
     6301 cccgacgatg gcccagggaa gctgcacctg ctcgagggca gcccgatcct tgcgcggcaa
     6361 ggcatagagc cagagctgct cgccgcgcag ctcggccggc tgcggcaggc ggaaatcgcc
     6421 ccacggcccg agcgcctcga tcatctggtc gagctcgaag gagcggtcgg cgagccggcg
     6481 caggcgcagg cggttctcga tgatctcgcc gatcacggca tcggcgtcga agggcttgcc
     6541 gagcggccag ggcgtcaact gctcgggcgc gtcctggaga tggtgaaagg cggtgtcggc
     6601 gcggcgccgg gcggccgggt cgtccggggt caagggcccg ggccggcgca acgggatcag
     6661 gtgcagcgcg cccagcgcct gcagctcctc gaggaccggc gccttctcgt gggcgaggcc
     6721 gcagagggtc accttgagca tgggggcgat ggtcaagcgc ccgcctccgg tcgcgcggcc
     6781 tcggcctcgc gcttgcgctt ggcgatcttc gccccggcga cggccgcccg ctcggcatcg
     6841 ccgagataga cattgatgcg ccggatgttg gtccgggtct gcgggatcag caccttgtcg
     6901 aagaggttca cccgctgggt gatccgggcg accgcccgct ccagcccggc gaggcgcttc
     6961 tcggcgacct ccacctcgac gcgcaggcgc atcgccgccg ccagccggtc ggccacgtgg
     7021 tcgacccagt gcgggcggac cagccggcca taggcgctgc ggatcagctc gacatgctcc
     7081 acggtcggca gcttcacgcc ggcgacgttc tgcgtgccca gccggacctc gcccagccga
     7141 accagcccgt cgagcgacac gcgccggtcg gccatcatcg ggatcgcctc gcccgccgcc
     7201 tcgatcacgc gggcgacctc gagcccgagc cgctcgatct cccgccgcgc ccgctgccgc
     7261 tcgcccatca gttgctggcg cttgagatcg agcgccggca agtagcgctg gtagagcgcg
     7321 agattctcct tctcgcgggt cagcgacgac ttgctgagct ggacctgggc catcaggcgg
     7381 cggacctcgg gtagtacttg tcgatcaggt tctggcgcac gagcagctcc tcgggcgcga
     7441 agcacgcggc catcgtctgc cagccgaggt cgagcgcctg ctcgatgggc agggccacgt
     7501 cgagatccat gaaacggctt tcgaagaagc tgccgtagcg cagcaggcgc tggtcgagat
     7561 cggagagctc gaacgccatg gcctgcttgc gctgggcgtc ccgggcgccc gagtagaggc
     7621 ggatcatggt gttcatgacc gccgagtggt cctccctcgt ctccttgccg atgacgtgct
     7681 gcttgagcct ggagagcgag ccgaacgggt cgatgcggcc gttgcgcagg tagaactggc
     7741 cttcggtgat gtagccggtg ttgtccggca cgggatgggt gacgtcgccg cccggcatgc
     7801 tggtgacgct caggatggtc accgagccgc cgccggggaa gaccgccgcc ttctcgtagc
     7861 gtcgggcgag ctgcgagtag aggtcgccca gatagccgcg ggtcgacggc acgcggtcga
     7921 gcgagatgcc gacctccttg agggcatcgg cataggcggt catgtcggtg agcaggacga
     7981 ggacgcgctt cttctcctcg accgcgaagc gctcggccac cgccagcgcc atgtccggga
     8041 tcaggaggcg ctcgagcgcc gggtcggaag cctggttgac gaacatcacg gtgcgcatga
     8101 acacgcccgc gtcctcgaag gtcgtccgaa acgtccagta gtcgtcgaag gtgaggccga
     8161 gcccgccgaa gacgacgacg tccgcctcgg cctggatgcc gatccgcgcg agcagcgcgt
     8221 tgtgcggctc gccggccgag gtgaagatcg ggatcttctg gctctcgacc agcgagttga
     8281 agacgtcgat catcggcacg tcggtgcgga tcatgccgga cgccatggcg cgccgcgccg
     8341 ggttggccga ggggccgtcg acctccacct tgggctcgtg atcgagcgag gggccgcggt
     8401 cgagcacgcg gccgtcaccg gcgaacaccc gccctagcac gctcggcgag aagaccgcct
     8461 tcggtgcgtg gcccaggaac cggacgcggc tgcgtgtcga caggccctcg accccggcga
     8521 agacctgcag cgacgcctcg ccgtcaccga cgcgcacgac ctgagcgagg cgcggcaggg
     8581 tgtccccctc gctgaccact gcgaggtcgc cgaagcgcgg gccctcgggg tcgtcggcgg
     8641 tctccttcgg cagggcgatg cggatcaccc cgccgatgat cgacagcacc cggtcatagg
     8701 ttatcgcgat gtccgccaag ccgtcgtctc cccgtcagcc caccatgccg tcgctcagca
     8761 atcgacccac accacggcga gtccgccctg ggaggtctcc ttgtacttgt cggacatgtc
     8821 cttgccggtg aggtagagcg ccttgatcac ctcgtcgaag ctcggccggg ccggctcgtc
     8881 gccacgcagc atggtcagcc ggacggcgtt gaccgccttg ttggcggcga gcgggttgcg
     8941 gttgatgcag ggcaccacca cgaggccctt caccggatcg caggtcaggc cgagattgtg
     9001 ctcgagcgcc atggcacctg cgttcagcac ctgggtcacc gagccgccga gcgcctgggt
     9061 caggccggtc gcggccatca ggctcgccgc cccgacctcg ccctggcagc cgacctcggc
     9121 cccggagaac gaggcgttga tcttgaccgg gatcgagcag ccgccggcgg cgagcaggaa
     9181 gtcgcagacc ccgtcgtcgc tggcgccggg cgcgtactgc ttgtaggcct tcagcaccgc
     9241 cgggacgatg ccggccgagc ccatcgtcgg ggcggtgacc acctggccga gcgaggcgtt
     9301 cagctccatc accgccagcg cataggcgtt gatgtggtcg gaaagctcga agaggtggcc
     9361 gccgcgcgtg ccctccttga cctggctcat gatgtccttg gcgatgcgct tgacgccgag
     9421 gccgcccggc acctcgccct cgccctggag ccccacctcg atcgatttct ccatgacggc
     9481 ggcgatctcg agcaggcggt cgcgcacggc cttctccgtc cgccagacga gctcgttctc
     9541 gaacacgatc tgggcgatcg tcttgccctc gcgggcgcag agcgccgcga gctcggcgcc
     9601 ggtcgtgtag gggaacggct tggagttgtc gccgatggcg gcggtcgggt ggatcgaatc
     9661 gctgtcgccg acgatgaagc cgccgccgat cgagaagtag agcgagcggt gcagcacctg
     9721 gcccgcgccg tcggtggcgg cgaaggcgat gccgttgggg tgcttggggt aggtcttgtc
     9781 gccccgccag tggatatgct tgtccgggtc gaacggcacc atccgccggc cgccgagatg
     9841 cagggtcctc tcccgctcga tccgctcgcg cctggccggg atgccggcgc tgtccagggt
     9901 ctcggggtcg gccccctcga gcccgagcat cgaggcgagg tcggtgtagt ggcccttgcc
     9961 ggtgagcgcc atcgagccgt agagctcgac ctcgacgccg ccgacggcgt cgccgaggcc
    10021 ggcctcctcg agctcgccga gaaagcggct ggccgccacc atcgggccga aggtgtgaga
    10081 cgaggagggg ccgacgccga tcttgaagat gtcgaagacg ctgatcgcca tgctgtcgtt
    10141 ccctgattgt ctgcggccgc cgcggcggct cagtagacgg tgaggccacg ttccctgaat
    10201 tgcgcgcgca cccgctcggt gagctcgggc gacggcggct cggcatcggc gagcgggtag
    10261 gggatgccga gcgtctcgta cttggccttg cccatctggt gaaagcgcag gatctcgacc
    10321 cgctcgagcg gctcgaggcc ggcgcagatg tcggccaccc tggcgacgtt gtccgggtcg
    10381 tcggtgaggc cggggaccag gacgaagcgg acccagagcg gccggccgag atcggagagc
    10441 cgccgggcat agtccagcgt cggctgcagc ggatggccgg tgacccgctc gtaggtcgcg
    10501 gggtcgcccg acttgatgtc gagcaactgg aggtcgatgt cggccaggtc cgcatccgtc
    10561 aaattgcggc ccaggcggcc cgaggtgtcg aggcaggtgt gcacgcccat ggctttcgtc
    10621 gcctgcatga cgcggcgcag gaagcgcggc tgcatggtgg gctcgccgcc ggagacggtg
    10681 agcccgccct tcgacagctt cagcaccttg gcgtacttgc cgatctcgcg ggtgatgcgg
    10741 ccgagcgtgt accgcttgcc gttgcgcttg tgccaggtgt cggggttgtg acagtactgg
    10801 cagcgcaagg ggcagccggt gaggaagagg acgaagcgga tgcccggccc gtcgacggtg
    10861 ctgcccacct cgatcgagtg gacgtagccg ccgacctcct cgtcggcctc ggcttccaga
    10921 tcctccggga acacctccgc atagtcgcgg cccttcgcct cgacggcgcc ggtgcgcgtg
    10981 cggcgcagct cgtagcggct gccgatgagc tcgctcatcg cgtcagaatt tgccgtggaa
    11041 ggtgcggttc aggacatcga gctgctgctc cttcgtcagc ttgacgaagt tcacggcata
    11101 gcccgagacg cggatggtga gctgcgggaa gtcctcgggt cgctccatgg cctcgcgcag
    11161 cgtgtcgcga ttcaggacgt tcaggttcac gtgaaacccg ttcgagccga aataggcgtc
    11221 gaggccgccc accgccttgg cgatccgctc ctcggccgag ccgagcgagg tggggaccac
    11281 cgagatggtg agcgagatgc cgtcgagggc atcgtcatag ggcagcttgg ccaccgacat
    11341 catggcggcg agcgccccgc gggtgtccgc gccattggag gggttggcgc cgggcgcgaa
    11401 gggctcgccg ctgcggcggc cgtccgggct gttgccggtc tgcttgccgt agacgacgtt
    11461 cgaggtgatg gtcaggatgg actgggtgtg cagggcgtcg cgataggtcg ggtggttgcg
    11521 gatcttgtcc atcagcatgc gggtcagcat caccgcgatg gcgtccgccc ggtcgctgtt
    11581 gttgccgtat ctcgggaact cgccttcgac ggcgtagtcg acgacgaggc cgcgatcgtc
    11641 gcggatcgcc cgcaccctcg cgtatttcat cgccgacagg ctgtcggcgg tgtgcgacag
    11701 gccggcgagg ccgcaggcca tggtcctcag cacgtcgcgg tcgtggaacg ccatgagcgc
    11761 cgcttcgtag cagtacttgt cgtgcatgta gtggatgatg ttgagcgcgt tgacgtaggt
    11821 gcgcgccagc cattcggcca tgacgtcgaa gcgcgccatc acctcgtcgt agtcgagcac
    11881 gtcggcggtg atcggcgcca atggcgggcc gacctgggcg cccgagatct cgtcccggcc
    11941 accgttgatc gcgaacagca gcgccttgag caggttcacc cgggcaccga agaactgcat
    12001 ctgcttgccg gtcgccatgc cggagacgca gcaggcgatc gaggcgtcgt cgccccattt
    12061 cggccgcatc aggtcgtcgt tcatgtactg gatcgaggag gtgtcgatcg agcacctggc
    12121 ggcgaaggac ttgaagccgt cgggcaggcg tgtcgaccag ccgacggtga ggttgggctc
    12181 gggcgccggg cccaggttgt agagggtctg gaggatgcgg aagctggtcc tcgtcaccag
    12241 cgcgcgcccg tcgtcgccca tgccgccgat atagtcggtg acccaggtcg gatcgcccga
    12301 gaagagctcg ttgtaggcag gcgtgcgcag gaagcggacg atgcggaact tgatcaccag
    12361 atcgtcgatc agctcctggg cgccggcctc gtccagggtt ccctcgcgga tgtcccgctc
    12421 gagatagatg tcgaggaagg tggtgatccg gcccacggac atcgccgcgc cgttctgctc
    12481 cttgatcgcg gcgaggaacg cgaaatagag ccactgcacc gcctcgcggc cggtcctggc
    12541 ggggccggcg acgtcgtagc cgtagcgctc ggccatgacc cggatctggc gcagcgaccc
    12601 gagctgctcg gtcagctcct cgcgcagccg gatgatctcc tcggtcgcat ggcgggagtc
    12661 gagctcggcc ttctcccgct ccttgtcctc gatcaggaaa tcgaggccgt agagcgccag
    12721 cctgcgatag tcgccgatga tccggccgcg gccataggcg tccggcaggc cggtgatgac
    12781 gccggagcgg cggcatttga ggatttccgg cgtgtaggcg ctgaacacgc cgtcattgtg
    12841 ggtcttgcgg taggtggtga agatcctggc caccgccggg tccggctcga agccgtaggc
    12901 ctcgaggctg tcggccacca cccgccagcc gccgctcggc atgatcgcgc gcttgagcgg
    12961 ggcgtcggtc tggagcccca cgatcagctc gagctccttg tcgatgtagc ccggcccgtg
    13021 ggcgatgatg ctcgacggct cctggctgac gtcgagcacg cccttcctgc gctcctcggc
    13081 gaggagcggc gtgagggcct cccagacctt ctgcgtccgc ggcgtggcgc ccgcgaggaa
    13141 cccggcgtcg cccaggtagg gctcgacgtt gcgctggatg aagtcacggg tgtcgacgcg
    13201 gtcgcaccag aggcccggct cgaagccgcg ccacgcctcg gcgaagcccg ggcgctcggg
    13261 gcggtggtga aaggtcatcg ccgggtctcc aggagcaggc tcatcgaagc ggggttgcgg
    13321 tcttgcggcg atgcgccacg agggcggaga cggctgccga ggcgagccgg ctctgcacgc
    13381 tgtcggcgcg gctggtgagc gtgatcggca cgcgcgcgcc gagcacgatc ccggcgctgt
    13441 cggcgtcgat caggaagccc aggctcttgg ccagcatgtt gccggcctcg aggtcggggg
    13501 ccaccaggat gtcggcatgc cccgcgacgg gcccgccgat gcccttgagc ctcgcggctt
    13561 cggggctgat ggcgttgtcg agggcgagcg ggccttcgag cagcccgccg gtgatctggc
    13621 cgcgatcggc catcttgcac aaggccgcgg cctcgaccgt ggtccccagc ttcgggttca
    13681 ccttctcggt cgccgaaagg atcgccacct tgggctcggg gatgccgagc gagtgggcca
    13741 ggtcgatggc gttctgcgtg atgtcgacct tctcctccag cgtcggcagg atgttgatgg
    13801 ccgcatcggt gatgatcagc ggccggccat agccgggcac gtccatgatg aagcaatgcg
    13861 acaggcggcg ctcggtgcgg agcccgtcgc tgcggctgac cacggcgccc aggagctcgt
    13921 cggtcgagag cgagcccttc atcagggtct cggcccggcc ctcgcgcacc agggccaccg
    13981 ccttggccgc cgcgtcgtgg ctgtgcgccg cgtcgacgat ctcgtacggc gcgagatcga
    14041 cgccggcagc gccggccgcg gcctcgatcc tggcccgcgg cccgacgagg atgggcagga
    14101 tgatgcccat ctcggccgcc tgcacggcgc cggcgaggga agactcgtcg cacgggtggg
    14161 ccacggccgt cggtgacggc ggcaccgcgg ccacggccgc gatcagacgg gcgtaagccc
    14221 tgcgctcgag atcggccaac aagccccctt tcgccccttc gccggcgcgc ccggcgccac
    14281 gccccgccgc tcgcggggaa ttccaggacg ccgatcgtgg cgcgcgaccg gggcttttgc
    14341 cagagccctt cgacggcgtt cctttgcgcg ccgtctcgat cagaacgcgc tgcaaaggaa
    14401 cggcccgggg cggccgcact ggccacttgc ccgaccgcga aaaaagacgt gaaggtcggc
    14461 gcgcgcgggc ctagctgccg cgccacccga tcgtccggcg caacttcgcg agaggcacca
    14521 tgtccgacgc cgccatcgtg gcgctgatca tcccggcggc cctggccctc ttcatctgga
    14581 acagggtgcc ggcggtgatc gtcggcgtcg gcgtctcgct ggcgctgttc gccaccggcg
    14641 tgatcacggc cagccaggcg ctggcgggcc tgggcgatcc cacggtcatc ctcatcgccg
    14701 gcctcttcgt cgtcgccgcc gggctcgagg cggcgggggt caccacctgg gccgggcaga
    14761 agctggtcga gacagcgggg ggcagcaaga gcctcgctct cctgctggtg atgctgatgg
    14821 ccgcgatctt ctgtgcgatc atcagcgtca acgccacggt cgcggcgctc ctgccggtgg
    14881 cggtgatcgt cgccatccgc ctcggcgtgc cgacctcgca gctgctcctg ccgatgtgct
    14941 tcggggcgca cgccggcacg atgctgaccc tgatcggcgc gccgctcaac gtcatcgcct
    15001 acgacgccgc gcgcgacgcg ggctatggcg ggatcggctt cttcgagtac gcggtggcgg
    15061 gcctgccgat catgatcggc tcgctggcga tcatgtacct gacccagcga ttcctcctgc
    15121 cgcaccgcga cgggctgaac ctgccggccg atttcagcag ccatgccgcg accctggtcg
    15181 agcagtatcg gctggaggac ggcctgcacc ggctgcgcct gcgcgagacc tcgccgctcg
    15241 tcggccagcc gcgctctgcc gtcgacctcg cccgctatcc ggggctcgcc ttcgtcgcga
    15301 tcgaggacgc gagcggcggg gtctcgacgc gcgacagcct ggccgccggc gacagcctgc
    15361 tcgtgcgtgg cgatgccgac tgggccggcc gcctcgccgg cgacctcggg ctggcgatcc
    15421 gcgaggcggc gggcgacgcg gcgcagaccc tcttcaaccg cggctccggc ctcgccgagg
    15481 tcgtcatccc gccgcgctcg ggcttcatcg gcgagacggc gttccccggc atggcgacgc
    15541 cgagcgggga tctgatgatc ctggcgatcc agcgtggcgg cgaggatctg ggcatcgagc
    15601 cggtcaccat cgcggccggc gacaccttgc tgctgcaggg tacctgggcc gccttcgacc
    15661 agcgcctcgc cgacccgcgc gtcctcgccg tccactcgcc ggagctggtg cgtcgccagg
    15721 cgatcccgct cggccaccgg gcctggcagg cgctcggcgt gctcctgctg ctggtcgtcc
    15781 tcctggtcac ggcccccttc ccttcggcca tcacggccgt gatatgcgcc gggctgatgg
    15841 tggtgctcgg cgtgctgacc gtgccgcaga tgtggcgcgg ggtggactgg aacacctgca
    15901 tcctggtcgg cggcatgatc ccgctcgccg gcgccatgac ccagagcggg ctcgcccagg
    15961 acatcgccga cggcttgatc ctgctgatcg gctgggccgg cccgctcgcc gtgctggccg
    16021 gcctgttcgc cgtcacggcc atcctgacgc aggtgatgag caacaccgcc acggccctcg
    16081 tcatgctgcc ggtcgccgtg gccagcggcc ccgagatggg ggtctccgcc atgccgatga
    16141 tcatcgcggt caccgtcggc gcgcacgccg ccctcctcac gcccatggcg accccggtga
    16201 acctgatggt cgtgggccct ggcggctaca cgttcggcga ctactggaag tacggcttgc
    16261 cgatcctcct ctggtggctc gtggtctgcg tgctgatcgt cccgctctgg tggcggttcg
    16321 acgcggcacc gctgtgaccc gggcgcgcca ttgcggctcg tcgcgcagcc cggtccgctc
    16381 cccgcctggt cttgtcagtg caccaaaata gcgctaatgg cgccccgcaa cctgcgcaga
    16441 aggcaggggt gccgacgaaa gacggcaaac tcgggcggct gggcggcaac agggatgtca
    16501 gacaggcgag cgccggcgac ggggctattg ccggaggcgc ggcagacggc ggccggggcc
    16561 cgcaacgatc ccgggcagca ggacgggcgc gctcgacccg tcatcagctt cgccaccgac
    16621 acgttccccg ccgagctcgc gttcgaggca tggcgggagc agttcggcca gctcaacgag
    16681 gtcatcgtgg ccaggggagc cggcttttcg ggccggtcgc gcttttggca gctcggcgac
    16741 ttcacggtgg cggtcaacga gacgtcggcc atgcgcctcg ttcgcagcgc ggcgcatctg
    16801 cggcgcgacg atctggacca ttggctgctg cgggtctcgg ccgagggcgt cgttcgctcc
    16861 cggtccggtg agcgtgcctt ccggagcgct cccggcgagc tggtggtcga gcggctgaac
    16921 gaggcctacg aggacgagtg gtcggccgac cgctgggtca tgctggtggt gccccccggc
    16981 acctgccccg agatcgaggc cggtctggcg gccatgccac gcgggccggt gggcggggtc
    17041 gaggcggcgc tgctcgccga cttcctcctg tcgctcgcca ggcggctgcc cgaggcgctg
    17101 gagagcgatc tgccggccct cgcccacacc acccagacga tgatcgccgc gaccttcgcc
    17161 gggcgtcatg gcgcggcgcg cgagagcccg cttgcggcgc gattgcgggt cgagcggacg
    17221 attcgcgcca gccttgcgtc cgcccgcctc gacccctcgc gcatcgccgc tctcgccggc
    17281 gtctcgcgcg cgacgctcta ccggctgctc gaggatcagg gcggcgttgc cgcgtatgtg
    17341 cgccgcctgc ggctggaggc ggtgcatgct gccctgtgca acctcgggca ggcccatgtc
    17401 ccgatcgccc ggctcgccga gcgggcggga ttccattcgc cggctgcctt caaccgcacg
    17461 tttcgtgccg ccttcggcgc ctcgcccggc gaggtgcgcg agcgggccgc ccgccaggac
    17521 ccgccgctgc cccgggcgcc cgacagctcg ccattccacc gcggctttct cgacctggcg
    17581 ttcccggctg gcgcccccgc gcattgaggg gccgaggggc tgctggtttt tcatctgtcc
    17641 gaacgcgaag ggccgccccc tttt
//
LOCUS       DAOTFI010000098        17615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3871_length_17615_cov_6.692825, whole genome shotgun sequence.
ACCESSION   DAOTFI010000098 DAOTFI010000000
VERSION     DAOTFI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17615
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_17615_cov_6.692825"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="P5558_11530"
     CDS             <1..747
                     /locus_tag="P5558_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY24986.1"
                     /translation="HVKAVDGVTLEVRAGQTVGVVGESGSGKTTLGLALLRLLPCTGS
                     IDFSGQAIADWSGAKLRSLRREMQIVFQDPYGSLSPRMSVGQIIEEGLVLHRIGAHAT
                     ERSELVAAAMLEVGLDPSMRDRYPHEFSGGQRQRISIARAMVLKPRFVVLDEPTSALD
                     MSVQAQIVDLLRDLQVRHGLAYLFISHDLRVVRALSHEVIVLRRGRVVEQGTAAQLFA
                     APATAYTRALMAAAFDIEAVDPGGAGGSVA"
     gene            complement(736..2127)
                     /locus_tag="P5558_11535"
     CDS             complement(736..2127)
                     /locus_tag="P5558_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY24987.1"
                     /translation="MTKRAAHHLVIGLALSVLTGGMSLAADDAPADWRELNDRGTAAA
                     AAEDWSEAESALRAALALLEPEAPGTAATPDDDARIAVVAANLAMVELAQGDTAAARA
                     LLERALAIRRAVFGDSEPGIAALLNNLGELERRAGNLDRARQLHEAALVMRRTLREPG
                     HPDIAESLNNLGVTLSDLGQLDTAGGLVQDALAMRRERLGPTHRATLESTANAAAIAT
                     EAGDLAAAETLLRAPLEAMVAASGDAHPDAELVAQLWRNLVEVLLLQNADDAALLACE
                     ELVADLPPADRQATTDLVTVCVRAFNRVGAEERATRLLEQQLELDEVPRAARLQLRWS
                     LAETAASAGDFEAAHEQIDTVIELLGTATDRRLPLALNNRAYFAFELGRPLDAATDLE
                     AALAAIEALPPPGDAALERDVLNNLASVLRSLDRGDEAAAVEERLLDTVPADAPTDGS
                     GAQPAAGPPPSGD"
     gene            complement(2133..3917)
                     /locus_tag="P5558_11540"
     CDS             complement(2133..3917)
                     /locus_tag="P5558_11540"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HRY24988.1"
                     /translation="MAENEKRRLRSQDWWDNPHEADMTALHLERQLNFGLTREELQSG
                     RPIIGIAQTGSDVAPCNRHHIDLAKRVADGIRDMGGVPLEFPIHPVQETLKRPTAMLD
                     RNLQYLSLVEILYGYPIDGVVLTTGCDKTTPACLMGAATVNIPAIVLSGGPMLNGYYK
                     GELAGSGMVVWHARQLMAKGEIDYEEFMAMTCASAPSVGHCNTMGTASSMNAMAEALG
                     MSLPGCAAIPGPYRERGQMAYATGKRIVEMVHEDLKPSEILTRKAFENAIFAAAAVGA
                     STNCPPHVIAIARHMGVELDIKDWEMGFDIPLLVDMQPAGRFLGEDYYRAGGLPAVVK
                     ELMGKGRWHEDALTVTGKSYGENIKGLDLYIDRNVIRPYDDPLMPQAGFVIISGNIFD
                     SAVMKTSVVTKEFRQKYLEHPDHPGVFEGRAVVFEGPEDYHERIDDPSLEIDEDCFLF
                     IRNCGPVGYPGAAEVVNMAPPAALIKKGIKALPTIGDGRQSGTSESPSILNASPEAAI
                     GGNLAIVQTGDRVRFDLPNRHLDLMLDAAEIERRQAKIETKVSPSQTPWQEIQRSMVG
                     QLSTGACLEPAVMHLRVVQTHGTPRRNH"
     gene            4212..5132
                     /locus_tag="P5558_11545"
     CDS             4212..5132
                     /locus_tag="P5558_11545"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRY24989.1"
                     /translation="MPVAVSGQERRRLHFLIPAASGGGWDQTARAVAESLTRTRLVDT
                     VTFEHLSGEGGGRAIAYMVATAERQQGTLMVSSTPIVLRAVRGAAKSTYHELTPIAAL
                     IGDYAAIAVRSDSPHRDFASLAAAVKRDPSSTKVAGGSVRGGMDHLVAVRAFSLAAGV
                     NPLNVVYYPYDAGGEAFDALLAGQTEALATGLGELVASPRRGEIRILAISAPERVPEV
                     PDAPTMRELGHDVTFVNWRGFFGPPNLPAAVAASHAGLLADLNRSPDWAEVRRRYAWQ
                     PLYHSGAEFAAFLAAEEATARQVLASLELI"
     gene            5248..6729
                     /gene="guaB"
                     /locus_tag="P5558_11550"
     CDS             5248..6729
                     /gene="guaB"
                     /locus_tag="P5558_11550"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRY24990.1"
                     /translation="MSPASEIAIRDALTFDDVLLEPAYSEVLPAAVDVQSRLAPDIPL
                     RIPLLSAAMDTVTEAPMAIAMAQLGGLGIIHKNLDIAEQAAHVRQVKKFEAGLVVNPV
                     TVTPQTPLADALQLMERNRISGLPVVEGPEKRLVGILTNRDVRFVPSSSRPVQELMTS
                     ENLVTVREGVARQDAMELLHRHRIEKLLVVDDAGRLIGLVTVKDIEKAERFPLAAKDG
                     QGRLRVGAATGTGSAGIERSLALVDAGVDVLVVDTAHGHSRGVLDAVAQVRRDTNAAR
                     IVAGNVATGDGAQALIDVGADAVKVGIGPGSICTTRVVAGVGVPQLTAILDVAARCRP
                     QRIPLIADGGIRSSGDLAKAIAAGADCVMLGSMFAGTDEAPGEVFLYQGRSYKSYRGM
                     GSLGAMARGSADRYFQEEVRDQLKLVPEGIEGRVPYRGPVGNIVHQLVGGLQSAMGYT
                     GHASLAAMQQSCRFIRITVAGARESHVHDVAITREAPNYRQEL"
     gene            6848..8887
                     /locus_tag="P5558_11555"
     CDS             6848..8887
                     /locus_tag="P5558_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY24991.1"
                     /translation="MERCANVWRAGSYLFAAAAILAAGLALLAPAAAPLSLGLGHIWP
                     SGSALPAADGRPGLVLLALLLPLATESLLRFLRPPVRRTAILVCSVLGGVLIGVVEPA
                     LLMALALAMATATTTAASPDAATGPRQPSDLPLVAHSAEVQGDGALTFDRTGTILAAD
                     ATAREMLGGDPLGRSLYRYLPRLARSCPTVDSLIGHRFETELQHESTRFHRVEAVFWA
                     ADAPGAWSGQARLTSVAARAERLAELERLALHDALTGLPNRTLFGDRARQAIAVAERQ
                     GESFAVMLLDLDRFKQINDTLGHATGDRLLQAIGPRLVRALRKSDTLARLGGDEFAVL
                     LPPAVDFDKATASAERLTESLVDPITIDGMSLELGVSVGVALYPEHGTDLDTLMRNAD
                     VAMYKAKREQSGFSVFDTVQAIGSARRLTLQRDLRVAIERGDLRVLFQPRVAANGWTN
                     LGLEALVRWQHPIEGLLEPTDFLPIAEHTGLVMPLTLSVLNQCLEAQRSWRHLGIELP
                     VSINVSAKWLKDPAFPRILGLLLRNAQGRADQLMLEIPESAVMLDPEGTIRTLENVAK
                     LGVGIALDGFGTGYSSLPLLQRLPIKALKIDRTFVGELFEQQSASVIIRSIVRMAHGL
                     GLKVIAEGVESGRAANRLAGIGCDELQGLFVGRPMAGESVPEWLAEQAEQRATRV"
     gene            9179..9730
                     /locus_tag="P5558_11560"
     CDS             9179..9730
                     /locus_tag="P5558_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRY24992.1"
                     /translation="METLTRFVRAVDRLNDAIGRAAAWLTLAMVLVTFLVVVARYGFS
                     WGRVWIQETYVWMHGCIFMLAAGYTLLHDGHVRVDVFYRPASRRFKAWVDLFGSLLLL
                     LPVICVVLWVSIPYVEASWLRWESSREAGGLPGLFLYKTVIPVFCLLIGLQGLSLAGR
                     SVLVLAGHPEFAPEERTAAELGV"
     gene            9754..11112
                     /locus_tag="P5558_11565"
     CDS             9754..11112
                     /locus_tag="P5558_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY24993.1"
                     /translation="MSLVMFAVTCLVLMLGFPVAFSLAGTSLAFGILGWWLGIFDLRL
                     LGGLASRYFGVMVNEVLVAVPLFVFMGVMLERARIADQLLQTMGMLFARVRGGLAVSV
                     TIVGALLAASTGIVGATVVTMGLMSLPTMLKAGYDPKLACGTICAAGTLGQIIPPSIA
                     LVILGDIMQGVNSQAQLALGNFAPKPVSVVDLFAGAMLPGLMLVGLYIAWQIFVATRN
                     PEVAPPLVVDVDKGLLREVLTALLPPAVLIIAVLGSILGGIATPTESAAVGAVGAMLL
                     AVLRRQLDLAILRDVMQATTRISCMVFVILLGASVFSLVFRGLGGEEIVRDVLSAMPG
                     GAFGAMFVVMALMFFLGFFLDFIEIVFVVVPIVGPILLMMGLDPIWLGVMIAMNLQTS
                     FLTPPFGFALFYLRGVAPPEVTTMHIYRGIVPFVIIQLIGLCLLALFPALATWLPSVI
                     FG"
     gene            11109..11576
                     /locus_tag="P5558_11570"
     CDS             11109..11576
                     /locus_tag="P5558_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HRY24994.1"
                     /translation="MSEGPKARPFWQAKPLAALTAEEWESLCDGCGKCCLVKLEDEAA
                     PDTVYFTDVACRLLDLATCRCTDYASRSRLVPGCRRLGPDRLGLLELMPPSCAYRRIA
                     EGRGLPEWHHLVSGDRSAIHRAGRSVRGRAVSESEVAEDALEDRIVEWPHRDC"
     gene            complement(11595..12713)
                     /locus_tag="P5558_11575"
     CDS             complement(11595..12713)
                     /locus_tag="P5558_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY24995.1"
                     /translation="MNRRKIVKSLGAGGLASGVAAASSFPKPALSQGRQQWRLVTAWP
                     ANFPGLGTGANLLAELIGKMSDGRLTVEVFSAGEIVPAFEAMDAVGSGTAEMGHGAPY
                     YWQGKVNAVAYVSAIPFGLTAQEQNAWFYFGDGQRLADKIYAEMGTKFFPSGNTGTQM
                     GGWFNKEMNSLADYQGLKMRTPGLGGKVVAAAGGNVVNLPGGEIAAALQSGAIDAIEW
                     VGPYNDLAFGLHQSAKYYYYPGWQEPGAVLDCFVNLAAWEGLSDDLKAIVEVANRAVN
                     LAVLSEFAARNKESLEVLVNEHGVELRAFSDEILMGLGQLAGEVMNDDASKDPLSREL
                     MDSILKFRATAVPYGGIAEQAVMAARMLPYEFAQPSGG"
     gene            complement(12822..13553)
                     /locus_tag="P5558_11580"
     CDS             complement(12822..13553)
                     /locus_tag="P5558_11580"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRY24996.1"
                     /translation="MNEKTLSRSFYATQPGPCPYLPGRVEQRIVTPFSLDDGPALFDS
                     LNQAGFRRSHGFLYRPACPGCHACVPVRVIVDRFVPSRRFRRVEKRNSDLVATCAPAV
                     SSDEQYDLFKRYLEARHDDGGMARMNRDEYAELVERATAETFIVEFRLAGALVAVALT
                     DRLLSGLSGVYTFFDPDLAARSLGTYTILWHIAEAARAGLPFFYLGYWIDGTRKMRYK
                     TQFRPIEQLTATGWRPLPPGTGTAA"
     gene            13875..14204
                     /locus_tag="P5558_11585"
     CDS             13875..14204
                     /locus_tag="P5558_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24997.1"
                     /translation="MPSERNVVIRLSGWARTLVLRETGEPVELVHIEELDDRRRAFVE
                     LPSGLRTYVAEAALTSRVARLSEGALTLAGVAIAGALLGYSFNLPQDELTASSNPRPV
                     VVGALEG"
     gene            complement(14271..14924)
                     /locus_tag="P5558_11590"
     CDS             complement(14271..14924)
                     /locus_tag="P5558_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY24998.1"
                     /translation="MLRPAERFAVKLHDPIGAARAEAGRRAAAACRPAWLLLPMALVL
                     GLTAAWLAQLGRPAVETSRMAGEPSTPGRPAEIEPALGGDSAAGWLGDVLPASLLGTR
                     PAGRDAGWPVVLHRAGDGAFYADILVAGAALHARIDPTGAEARLAAADLPAGAVVEDE
                     RWLAAAVELQHLRLPEVAFRIVDAPTEAVLGMAGLKAAVEVEESPDRLRLVPTRRRR"
     gene            complement(15048..15881)
                     /locus_tag="P5558_11595"
     CDS             complement(15048..15881)
                     /locus_tag="P5558_11595"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF010005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HRY24999.1"
                     /translation="MYAYRWQRRYVGPVRAVIFDMAGTLMDFGSRAPAGVFVEVFRRR
                     RVAMTVAEARGPMGAEKRRHIEMLLELPAVAERFAAAHGRAWSQQDVDELYAAFIPLQ
                     IESLPAFAGLVPGALDVGQALAERGIGIGVNSGYSRDMLEVCLAEAKRQGFAWQSAVA
                     ASDVPRARPCPAMCLKNAAELAVADVAACVKVDDTVPGILEGLAAGMWTVGLAISGNE
                     VGLSHADWQALRPAEQEKRRERAVARLAEAGAHYVVDSVADLLPCIAAIEKRLGRGER
                     P"
     gene            complement(15914..17026)
                     /locus_tag="P5558_11600"
     CDS             complement(15914..17026)
                     /locus_tag="P5558_11600"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HRY25000.1"
                     /translation="MTQHQRLLTPGPLTTTERVKRAMLRDWGSRNADFIALNRAVLDR
                     LVELIGGGDEHVAVPMQGSGSFAVEAMLGTLVPRDGKLLVMVNGAYGARMVEMARYMG
                     RAVVAIEDPEDRPGDTRRLEETLAADPAIGHVAAVYCETTSGILNPIDEIARIAARHG
                     RALLIDAMSAFGALPLDAARIRFEAVAASANKCLEGVPGMGFVLARKDALAAAEGNAH
                     SLTLDLAAQHAAMVRTGQWRFTPPTHVLAAFHEALVQHEEEGGVEGRGRRYRENCRVL
                     VDGMRRLGFQPLLEEALQAPIIVTFRMPRDPAFDFLQFYDRLAAKGFVIYPGKLTVAP
                     SFRIGCIGALGADDMHAAMAAIEATLGELGVANSRP"
     gene            complement(17142..>17615)
                     /locus_tag="P5558_11605"
     CDS             complement(17142..>17615)
                     /locus_tag="P5558_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY25001.1"
                     /translation="VDLGELVQDIFDLYEPLAEERDIGLALENAAGITVAGNRQMLAQ
                     ALANLTDNAIKYTPEGGHVTLAAAQTREQARRIARITVSDDGPGIPPEMRQKALERFV
                     RLEPERHTPGNGLGLSLVSAVAKLHDARLELADNEPGLRVSLVFERPVETAPPGA"
BASE COUNT         2461 a         5984 c         6258 g         2912 t
ORIGIN      
        1 catgtcaagg cggtcgacgg cgtgacgctc gaggtgcgcg ccgggcagac ggtgggggtc
       61 gtcggcgaga gcgggtcggg caagacgacg ctcggcctgg ccctgctccg gctcctgccc
      121 tgcaccggga gcatcgattt ctccgggcag gccatcgccg actggtccgg ggcgaagctt
      181 cgctcgctgc gccgggagat gcagatcgtc ttccaggacc cctatggcag cctgagcccg
      241 cgcatgtcgg tcggccagat catcgaggag gggctcgtcc tgcatcggat cggcgcccat
      301 gcgacggagc gctcggagct ggtcgcggcg gcgatgctgg aggtcgggct ggacccgtcg
      361 atgcgggacc gctacccgca cgagttctcc ggtggccaac ggcagcgcat ctcgatcgcg
      421 cgcgccatgg tgctcaagcc gcgcttcgtg gttctcgacg agcccacctc ggccctcgac
      481 atgtcggtcc aggcgcagat cgtcgatctc ctgcgcgacc tgcaggtccg ccatggtctc
      541 gcctatctct tcatcagcca cgatctgcgc gtcgtgcggg ccctcagcca cgaggtgatc
      601 gtcctcaggc gcggtcgcgt ggtggagcag gggacggcgg cacagctctt cgcggcgccg
      661 gccacggcct atacgcgagc gctgatggcg gccgccttcg acatcgaggc ggtcgatcct
      721 ggcggcgccg gcggctcagt cgcctgaggg cggcggcccg gcggccggct gggcgccgga
      781 cccgtcagtc ggcgcgtcgg ccgggaccgt gtcgagcagg cgctcctcga ccgccgcggc
      841 ctcgtcgccg cgatccagcg accgcagcac gctcgcgagg ttgttgagga cgtcgcgctc
      901 gagggcggca tccccgggcg gcggcaatgc ctcgatcgcc gccaaggccg cctcgaggtc
      961 cgtggcggcg tcgagcggac ggccgagctc gaacgcgaaa taggcgcgat tgttgagggc
     1021 gagcggcagg cggcggtcgg tggccgtccc caggagctcg atcaccgtgt cgatctgctc
     1081 gtgggccgcc tcgaagtcgc ctgccgaggc agccgtctcg gcgaggctcc agcgcagctg
     1141 gagcctggcc gcgcgcggca cctcgtcgag ctcgagctgc tgctccagga gcctggtcgc
     1201 ccgttcttcg gcaccgacgc ggttgaacgc gcgcacgcag accgtgacga ggtcggtggt
     1261 cgcctgccga tcggctggcg gcaggtcggc caccagctcc tcgcaggcga gcagggcggc
     1321 gtcgtcggcg ttctgcagca gcaggacctc gacgaggttg cgccagagct gcgcgacgag
     1381 ctcggcgtcc gggtgggcat cgcccgaagc ggcgaccatg gcctcgagcg gcgcccgcag
     1441 gagggtctcg gccgcggcga ggtcgccggc ctcggtggcg atggccgccg cattggcggt
     1501 actctcgagc gttgccctgt gggtggggcc gagcctttcc cgacgcatgg cgagtgcgtc
     1561 ctggaccagc ccgccggcgg tgtcgagctg tcccaggtcg ctcaaggtga cacccaggtt
     1621 gttcaggctc tcggcgatgt ccggatggcc gggctcgcgc agggtgcgac gcatgacgag
     1681 ggccgcctcg tgaagctggc gcgcccggtc gagattgccg gcacgccgct cgagctcgcc
     1741 caggttgttg aggagtgcgg cgatgccggg ctcgctgtcg ccgaagacgg cccgccggat
     1801 ggcgagggcg cgttccagga gcgcgcgcgc cgctgccgtg tcgccctgcg ccagctccac
     1861 catggcgaga ttggcggcga cgacggcgat ccgggcgtca tcgtcgggcg tggccgccgt
     1921 gcccggggct tcgggttcga gcagagcgag cgccgcgcgc agggcactct cggcctcgct
     1981 ccagtcctcg gccgccgccg cggccgtgcc gcggtcgttc agctcccgcc agtccgccgg
     2041 cgcgtcatcg gcggcgagcg acatgccacc cgtcaggacc gacagcgcca gtccgatgac
     2101 gaggtggtgt gccgctcgct tcgtcacccc tattagtggt tgcgccgtgg cgtgccgtgc
     2161 gtctggacca cccggagatg catcacggcg ggctccaggc aggcgccggt cgagagctgg
     2221 cccaccatgc tgcgctgaat ctcctgccag ggggtctggc tcggcgagac cttcgtctcg
     2281 atcttcgcct gacgccgctc gatctcggcg gcgtcgagca tcaggtcgag atgccggttg
     2341 ggcaggtcga agcgcacccg gtcgccggtc tgcacgatcg cgagattgcc gccgatcgct
     2401 gcctcggggg aggcgttgag aatcgacggg ctctccgagg tgccgctctg ccggccatcg
     2461 ccgatcgtcg gcagcgcctt gatgcccttc ttgatgaggg cggccggcgg cgccatgttg
     2521 acgacctccg ccgcccccgg gtagccgacc ggcccgcagt tgcggatgaa gaggaagcag
     2581 tcctcgtcga tctcgaggct cggatcgtcg atccgctcgt ggtagtcctc cggcccctcg
     2641 aagacgaccg cccggccctc gaagacgccc ggatggtcgg ggtgctcgag atacttctgg
     2701 cggaactcct tggtcacgac cgaggttttc atgacggcgg aatcgaagat gttgccactg
     2761 atgatgacga agccggcttg gggcatgagc ggatcgtcat aggggcggat gacgttgcgg
     2821 tcgatgtaga ggtcgaggcc cttgatgttc tcgccgtagc tcttgccggt gacggtgagc
     2881 gcatcctcgt gccagcggcc cttgcccatg agctccttga ccacggccgg cagcccgccg
     2941 gcgcggtagt agtcctcgcc gaggaagcgg cccgccggct gcatgtcgac gaggagcggg
     3001 atgtcgaagc ccatctccca gtccttgatg tcgagctcga cgcccatgtg ccgggcgatg
     3061 gcgatgacgt gcggcgggca gttggtcgag gcgccgacgg cggcggccgc gaagatggcg
     3121 ttctcgaagg ccttgcgggt caggatctcg gacggcttca ggtcctcgtg caccatctcg
     3181 acgatgcgct tgccggtggc ataggccatc tggccgcgct cgcgataggg ccccgggatg
     3241 gcggcgcagc cgggcagcga catgccgagt gcttcggcca tggcgttcat cgagctcgcc
     3301 gtgcccatgg tgttgcagtg gccgaccgac ggtgcggagg cgcaggtcat cgccatgaac
     3361 tcttcgtagt cgatctcgcc cttggccatg agctggcgcg cgtgccagac caccatgccg
     3421 gagccggcga gctcgccctt gtagtagccg ttcagcatcg gcccgccgga cagcacgatg
     3481 gccgggatgt tgaccgtcgc cgcgcccatg aggcaggcgg gggtggtctt gtcgcagccg
     3541 gtcgtcagga ccacgccgtc gatcggatag ccgtagagga tctcgacgag ggagaggtac
     3601 tggaggttcc ggtcgagcat cgcggtcggg cgcttgagcg tctcctgcac cggatggatc
     3661 gggaactcca gcggcacgcc gcccatgtcg cggatgccgt cggcgacgcg cttggcgagg
     3721 tcgatatggt gccggttgca cggtgccacg tcggagccgg tctgggcgat gccgatgatc
     3781 ggccggccgg actgcagctc ttcccgggtc agcccgaaat tgagctggcg ctcgaggtgg
     3841 agcgccgtca tgtcggcctc gtgggggttg tcccaccagt cctgactgcg caggcgccgt
     3901 ttctcgttct cggccacgag ccgtctccca tcgatccagt gaaaagcacg gtccccatta
     3961 ccatcactcg tcagagggct gcaatcgatc gctgcggcgt gcgggcggcg aaggggcggt
     4021 ggtcgacttt gaattttgtg gttgtatcca tcgcgccgac gttgaatcct gatgatcgcg
     4081 atttccatat ggaggccagc cggctcatga accgcaagac ccgacttcat cgtcagatgc
     4141 ccgccgcagc cgggggctgg tcacgacggc aagcactgca tggtctgggc gcgggccttt
     4201 tggccgcggg cgtgcctgtg gcggtctcgg gccaggagcg gcggcgcctg cacttcctca
     4261 ttccggccgc gtccggcggc ggctgggacc agaccgccag ggcggtggcc gaatcgctga
     4321 cccgcacccg gctcgtcgac accgtcacgt tcgagcatct ctcgggcgag ggaggcggcc
     4381 gggcgatcgc ctacatggtg gcgaccgccg agcggcagca gggcaccttg atggtgagct
     4441 cgacgccgat cgtccttcgg gcggtgcgtg gtgccgccaa gagcacctat cacgagctga
     4501 cgccgatcgc ggcgctcatc ggcgactacg cggcgatcgc cgtgcgcagc gacagcccgc
     4561 atcgcgattt cgcctcgctg gccgcggcgg tcaagcgcga tccctcgagc accaaggtgg
     4621 ccggcgggtc ggttcggggc ggcatggacc atcttgtcgc ggtgcgcgcc ttcagcctcg
     4681 cggccggggt caatcccctg aacgtggtct attatcccta tgacgccggt ggcgaggcgt
     4741 tcgatgccct gctggccggc cagaccgagg cgctggcgac ggggctgggc gagctcgtcg
     4801 cgtccccgcg ccgcggcgag atccgcattc tcgccatctc ggcccccgag cgggtgcccg
     4861 aagtgccgga tgcgcccacc atgcgcgagc tcgggcacga cgtcaccttc gtcaactggc
     4921 gggggttctt cggcccgccg aacctgccgg cggcggtcgc cgccagccat gcggggctgc
     4981 tggccgatct caatcgctcg cccgattggg ccgaggttcg ccgacgctat gcctggcagc
     5041 ccttgtacca ttcaggggcc gagttcgccg cgttcctcgc cgccgaggag gccacggcgc
     5101 ggcaggtgct ggcgtcgctc gagctcatct agccgcacgg cctccgaccc gggctttcgg
     5161 cgttgcagca atagctctat agaggcgcct gcaccgccga tcgccgacca gacgagctga
     5221 tcggcgtcca tcttccggga tccgaccatg tcgcctgcga gtgagattgc cattcgcgat
     5281 gccctgacct ttgacgacgt tctcctggag cccgcctatt cggaggtgct gcccgccgcg
     5341 gtggacgtgc agtcgaggct ggcgccggac ataccgctgc ggattccgct cctctccgcg
     5401 gccatggaca cggtgaccga ggcgcccatg gcgatcgcca tggcgcagct cggcgggctc
     5461 ggcatcattc acaagaatct cgatatcgcc gagcaggctg cgcatgtccg gcaggtcaag
     5521 aagttcgagg cgggcctggt cgtcaacccg gtcaccgtca ccccgcagac gccgctcgcc
     5581 gacgccctgc agctcatgga gcggaaccgg atctcgggcc tgccggtggt cgagggcccg
     5641 gagaagcggt tggtcgggat cctgaccaat cgcgacgtcc gcttcgtgcc gtcgagcagc
     5701 cggccggtcc aggagctgat gacctccgag aacctcgtca cggtgcgcga gggggtggca
     5761 cgtcaggatg cgatggagct cctgcaccgc catcgcatcg agaagctcct ggtggtcgac
     5821 gatgccgggc ggctgatcgg gctggtcacc gtcaaggaca tcgagaaggc cgagcgcttc
     5881 ccgctcgccg ccaaggacgg ccaggggcgc ctgcgggtcg gtgccgcgac gggcaccggc
     5941 agcgccggga tcgagcggtc cctcgcgctc gtcgacgccg gcgtcgacgt gctcgtcgtg
     6001 gacaccgccc atggccattc gaggggcgtg ctcgacgcgg tggcgcaggt ccgccgagac
     6061 accaacgccg cgcggatcgt cgccggcaat gttgccaccg gcgatggcgc ccaggcgctg
     6121 atcgatgtcg gcgccgacgc ggtcaaggtc ggcatcggcc cgggatcgat ctgcacgacg
     6181 cgggtcgtcg ccggggtcgg cgtgccgcag ctgacggcca tcctcgatgt cgcggcgcgc
     6241 tgccgcccgc agcggattcc gctgatcgcc gacggcggca tccggtcgtc cggcgatctc
     6301 gccaaggcca tcgccgcggg tgcggactgt gtcatgctgg ggtcgatgtt cgccggcacg
     6361 gacgaggcgc cgggcgaggt ctttctctac cagggccggt cctacaagtc ctatcgcggc
     6421 atgggctcgc tcggtgcgat ggcgcgcggc tcggccgatc gctacttcca ggaggaggtc
     6481 cgcgaccagc tcaagctcgt cccggagggc atcgagggcc gcgttcccta tcgcgggccg
     6541 gtcggcaaca tcgtccacca gctggtcggc ggcctgcagt ccgccatggg ctacaccggg
     6601 catgccagcc tcgcggccat gcagcagagc tgccgcttca tccgcataac ggtcgcgggc
     6661 gcgcgcgaga gtcacgtcca cgacgtggcg atcacgcgcg aggcgcccaa ctaccgccag
     6721 gagctctgag ccgctctttc tcatgcctgc tgcccggcgc cgcggtgcgc ctgacaaccc
     6781 aatcttaagc gtctcgcggt agattgcgtc tcgcaccggc ggcagaagca cccgctggcg
     6841 atgtgccatg gagagatgcg cgaacgtgtg gcgggccggg tcgtatctgt tcgctgctgc
     6901 ggcgatcctg gccgccggcc tggcgcttct ggcgccggcg gcagcaccgt tgtcgctcgg
     6961 gctcgggcat atctggccga gcggctcggc gcttcccgcg gccgacggcc ggcccgggct
     7021 cgtgctcctg gcgctgctgc tgcccctggc gacagaaagc ctgctgcgct ttctgcgacc
     7081 gccggtcagg cggacggcaa tcctcgtctg ctccgttctg ggcggagtcc tgatcggcgt
     7141 cgtcgagccg gcgctgctca tggcgctcgc tctggcgatg gcgaccgcca cgacgaccgc
     7201 cgcgtctccg gatgcggcca ccggtccacg acaaccgagc gacctgccgc tcgtcgcgca
     7261 ttcggccgag gtccagggcg acggcgcgct gaccttcgac cggacgggaa cgatcctcgc
     7321 agccgacgcc acggcgcgcg agatgctcgg cggcgacccg ctgggccggt cgctctatcg
     7381 ctacctgccg cgcctcgccc gaagctgtcc gaccgtcgac agcctcatcg gccatcggtt
     7441 cgagaccgag ctgcagcacg agtcgacccg tttccatcgg gtggaggcgg tgttctgggc
     7501 agcggacgcg ccgggcgcct ggtcgggcca ggcgaggctg accagcgtgg cggcgcgggc
     7561 cgagcggctg gccgagctgg aacgcctggc gctgcacgat gcgctcaccg gcctgcccaa
     7621 tcggacactg ttcggtgatc gtgcccggca ggccatcgcc gtggcggagc gccagggcga
     7681 atcgttcgcg gtgatgctgc tcgacctcga ccgcttcaag cagatcaacg acacgctcgg
     7741 ccatgcgact ggcgaccggc tgctgcaggc gatcgggccg cgactggtcc gggccctgcg
     7801 caagtcggat accctggccc gtctgggcgg ggacgagttc gccgtgctgc tgccgccggc
     7861 ggtcgatttc gacaaggcga cggcgagcgc cgaacgactg accgaaagcc tggtcgaccc
     7921 gatcacgatc gatggcatga gcctcgagct cggggtcagc gtcggggtgg cgctctatcc
     7981 ggagcacggc accgacctgg acacgctcat gcgcaatgcc gatgtggcca tgtacaaggc
     8041 caagcgcgag cagagcggct tcagtgtctt cgacacggtg caggcgatcg gcagcgcccg
     8101 ccggctgacg ctgcagcgcg atcttcgcgt cgccatcgag cgtggcgacc tgcgcgtcct
     8161 ctttcagccc cgggtcgcgg ccaatggctg gacgaatctc ggactcgagg cgctggttcg
     8221 ctggcagcat cccatcgagg ggctgctgga gcccacggac ttcctgccga tcgccgagca
     8281 taccggcctc gtcatgccgc tcacgctgag cgtgctcaac cagtgcctcg aagcccagcg
     8341 gagctggcgc catctcggca tcgagctgcc ggtgtccatc aatgtctcgg ccaagtggct
     8401 gaaggacccg gcctttccca ggatcctcgg tctcctcctg cgcaacgcgc agggccgcgc
     8461 ggatcagttg atgctcgaga ttcccgaatc ggcggtcatg ctggatccgg aaggcaccat
     8521 ccgcacgctc gagaacgtgg cgaagctcgg ggtcggcatc gccctcgacg gtttcggcac
     8581 cgggtattcg tccctgccgc tgctgcagcg cctgccgatc aaggcgctca agatcgaccg
     8641 gacgttcgtt ggcgagctgt tcgagcagca gagcgcctcg gtgatcatcc gctcgatcgt
     8701 caggatggcg cacgggctcg gcctgaaggt catcgccgaa ggggtcgaga gcggccgagc
     8761 ggccaaccgc ctggccggga tcggctgcga cgagctgcag ggcctcttcg tcggccggcc
     8821 gatggcgggc gagagcgtgc ccgaatggct ggcggagcag gccgagcagc gcgcaacgcg
     8881 ggtctgaggc agggccgccc gggcgggcgg agcagggcgc gcctggtcgc tggtgcgccc
     8941 ggctcggccg ctggcgacgc ggcgggcgac cgcggctcgc tccaggcggg ccgagctgct
     9001 gcctggtcat gccgtgcggg cttgtggggg tcggcctgtc ggccgggcgt gcccttgtgg
     9061 gtgcgtcgcc ggggtcgatc ggaccacgtt gcccagggag tgccggctgt gtacaagcag
     9121 cgacgccaaa tggtggccaa tcgtccgcct caggacctga tcgatctcgg gaccggatat
     9181 ggagactctc acgcgtttcg tgcgggcggt cgatcgactg aacgatgcca ttggccgcgc
     9241 cgctgcgtgg ctgaccttgg ccatggttct cgtcaccttc ctcgtcgtgg tcgcccgcta
     9301 cggtttttca tggggtcggg tgtggatcca ggagacctat gtctggatgc atggctgcat
     9361 cttcatgctg gccgccggct acacgctgct gcacgatgga catgtgcggg tcgacgtctt
     9421 ctatcggccg gccagtcgcc gcttcaaggc ctgggtcgac cttttcggct cgctgctcct
     9481 gctgctgccg gtgatctgcg tcgtgctctg ggtctcgatc ccctatgtcg aggcgtcgtg
     9541 gctgcgctgg gaatcgagcc gtgaggctgg cgggctgccg gggctcttcc tctacaagac
     9601 cgtgatcccc gtcttctgcc tgctcatcgg cctgcagggc ctgtcgctcg ccgggcgcag
     9661 cgtgctcgtc ctcgccgggc atcccgagtt cgctcccgag gagcggaccg cggcggaact
     9721 cggcgtttga gctcggcttt tctcggcgag gtgatgagcc tcgtcatgtt cgccgtgacc
     9781 tgcctcgtcc tgatgctcgg cttcccggtg gcgttctcgc tcgccggaac ctcgctggcc
     9841 ttcggcattc tcggctggtg gctcggcatc ttcgacctgc ggctgctcgg cggcctggcc
     9901 tcgcgctatt tcggcgtgat ggtcaacgag gtgctcgtcg ccgtgccgct attcgtcttc
     9961 atgggggtga tgctggaacg ggcgcgcatc gccgaccagt tgctgcagac catgggaatg
    10021 ctgttcgccc gggtgcgggg tggcctcgcc gtctcggtga ccatcgtcgg cgcgctcttg
    10081 gcggcctcca ccggcatcgt cggggccacg gtcgtcacca tgggcctcat gagcctgccg
    10141 accatgctga aggcgggcta cgacccgaag ctcgcctgcg gcacgatctg cgctgccggc
    10201 acgctcggcc agatcattcc cccgtccatc gccctggtca tcctcggcga catcatgcag
    10261 ggggtgaatt cgcaggcgca gctcgcactc ggcaacttcg cgccgaagcc ggtctccgtc
    10321 gtcgatctgt tcgccggggc catgctgccg ggcctgatgc tggtcggtct ctatatcgcc
    10381 tggcagatct tcgtggcgac gcgcaacccg gaggtggcgc cacctctggt ggtggatgtg
    10441 gacaagggcc tcctgcggga ggttctgacg gcgctgctgc cgccggccgt gctgatcatc
    10501 gcggtgctgg gctccatcct gggcggcatc gccacgccca ccgaatcggc cgccgtgggg
    10561 gcggtcggtg cgatgctcct cgccgtgctg cggcgccagc tcgacctcgc catcctgcgc
    10621 gacgtcatgc aggcgacgac caggatctcc tgcatggtct tcgtcatcct gctcggcgct
    10681 tccgtgttct cgctggtgtt tcgcggcctc ggcggcgagg agatcgtcag ggacgtgctc
    10741 tcggcgatgc cgggtggcgc cttcggtgcc atgttcgtcg tcatggcgct gatgttcttt
    10801 ttggggttct tcctcgattt catcgagatc gtgttcgtgg tggtgccgat cgtcggcccg
    10861 atcctcctga tgatgggcct cgacccgatc tggctcggcg tgatgatcgc catgaattta
    10921 cagacgagct ttttgacacc gccctttggt ttcgcgctct tctatctgcg cggcgtggcg
    10981 ccgccggagg tcaccacgat gcacatctac cggggcatcg tgccgttcgt gatcatccag
    11041 ctcatcggtc tctgcctgct cgccctcttc ccggccctgg cgacctggct gccctccgtc
    11101 atcttcggat gagcgagggc ccgaaggcca ggcccttctg gcaggcgaag ccactcgccg
    11161 ccctgacggc ggaggagtgg gaatcgctct gcgacggctg cggcaaatgc tgcctcgtca
    11221 agctcgagga cgaggctgcg ccggacacgg tgtatttcac cgatgtcgcc tgccgcctgc
    11281 tcgatctcgc gacctgccgg tgcaccgact atgccagtcg cagccgcctt gtgcccggct
    11341 gccgccgcct cggcccggac cgcctcgggc tgctcgagct gatgccgccg agctgcgcct
    11401 atcggcgcat cgccgagggg cggggattgc cggagtggca tcacctggtg tcgggcgacc
    11461 ggtcggcgat ccaccgggcc gggcgttcgg tgcgcggccg ggcggtcagc gagagcgagg
    11521 tcgcggagga cgcgctcgag gaccggatcg tcgaatggcc gcatcgggac tgctgagccc
    11581 gatgcggcac ggcttcaacc gccggaaggc tgcgcgaact cgtaagggag catgcgcgcg
    11641 gccatgaccg cctgctcggc aatgccgcca tagggcacgg cggtggcgcg gaatttcagg
    11701 atgctgtcca tcagctcgcg cgacaggggg tctttggacg cgtcgtcgtt catcacctcg
    11761 cccgcgagct ggccgagacc catcaggatc tcgtcgctga aggcccgaag ctcgacgcca
    11821 tgctcgttga ccagcacctc cagcgactcc ttgttgcgcg cggcgaactc ggaaaggacg
    11881 gcgaggttca cggcgcggtt cgccacctcg acgatggcct tcagatcatc cgagaggccc
    11941 tcccatgccg ccaggttgac gaagcagtcg agcacggccc ccggctcctg ccagcccggg
    12001 tagtagtagt acttggcgga ttgatgcagg ccgaaggcca gatcgttgta ggggccgacc
    12061 cattcgatgg cgtcgatggc gccggactgc agcgccgctg ctatctcgcc gcccggcagg
    12121 ttgacgacgt tgccaccggc ggccgccacc accttgccgc ccaggccggg ggtccgcatc
    12181 ttcaggccct ggtagtcggc gaggctgttc atctccttgt tgaaccagcc ccccatctgg
    12241 gtcccggtat tgccggacgg gaagaatttc gtgcccatct cggcatagat cttgtcggcg
    12301 agccgctggc cgtcaccgaa gtagaaccag gcattctgct cctgcgcggt caggccgaac
    12361 gggatggccg aaacataggc taccgcgttg accttcccct gccagtaata gggggcgcca
    12421 tgccccatct ctgccgtgcc actgcccacg gcgtccatcg cctcgaaggc cgggacgatc
    12481 tcgccggcgc tgaacacctc gaccgtcagg cggccatcgg acatcttgcc gatcagctcg
    12541 gcgagcaggt tggcgcccgt gccgagcccg ggaaagttgg cgggccaggc agtgacgagg
    12601 cgccactgct ggcggccctg ggacaacgcc ggcttcggga aggacgaggc cgccgccacg
    12661 cccgatgcca ggccaccggc tccgagcgac ttgacgatct tgcgacgatt caaagcgaat
    12721 actccctgcg cttggcccga tgatggatct gtcgggaacg gccagcggac gtcgcgggct
    12781 gcatagcggc tgggccgggc gctgacaatc ggcaggttgc ctcaggcggc ggttccggtt
    12841 ccgggcggaa gcgggcgcca gccggtcgcc gtgagctgct cgatcggccg gaactgggtc
    12901 ttgtagcgca tcttgcgcgt tccatcgatc caatagccga gatagaagaa cggcagtccc
    12961 gctcttgccg cttcggcgat gtgccagaga atcgtgtagg tcccgaggct gcgggcggcg
    13021 aggtcgggat cgaagaaagt atagactccc gagagacccg ataggaggcg atcggtgagc
    13081 gccacggcga cgagcgcgcc ggccaggcga aactcgacga tgaaggtctc ggccgtcgcc
    13141 cgctcgacca gctcagcata ttcgtcacgg ttcatccgcg ccatgccgcc atcgtcgtgg
    13201 cgcgcctcga gatagcgctt gaagaggtcg tactgctcgt cgctgctgac cgccggcgcg
    13261 caggtggcga cgaggtcgct gttgcgcttc tcgacccggc ggaaccggcg gctcggaacg
    13321 aatcggtcga cgatcacgcg cacgggcacg caggcgtggc agccggggca ggccggccgg
    13381 tagaggaagc cgtggctgcg gcggaagccg gcctggttca ggctgtcgaa cagggccggc
    13441 ccgtcgtcca gcgagaacgg cgtgacgatc cgctgctcca cgcgcccggg cagatagggg
    13501 cagggcccgg gctgggtcgc gtagaaggag cgggagaggg ttttctcgtt cacctggact
    13561 gcatggtcga tggagtgcgc cggccggcga ccggcggagc cgatcggcgg ctggccgcca
    13621 ttctgccacc aagcgtgtca aagcgcgacg gtgcgcacaa gcgtgccgtg cgcgttttca
    13681 agaacgatcc agggaaggcg ggaatgacca cccgccagcg tgcccaccga cacgcctcgc
    13741 tctgcgggtg acgttaacgg ttcattcatc ggcgttgctg tagggcttgt tccaccgcat
    13801 cggcgcttga ccgtggcgct gaacagaagt aggaacatga tcctacacta cagcttgtgc
    13861 tcgaggagcc gtggatgccg tcggaacgga acgtggtgat acgcctgtcg ggctgggccc
    13921 gcacactcgt gctgcgcgag accggcgagc cggtcgagct cgttcacatc gaggagctgg
    13981 acgatcgccg gcgcgccttc gtcgagctgc cgagcggcct gcgcacctat gtcgccgagg
    14041 cggcgctgac gagtcgggta gcccggttga gcgaaggtgc gctgacgctg gccggtgtcg
    14101 cgatcgccgg cgccctgctc ggctacagtt tcaatctgcc gcaggacgag ctgaccgcca
    14161 gcagcaaccc tcggcccgtg gtggtcggcg cactcgaggg ctaggagcct gtccgacgag
    14221 ttgtcggaag ggctcccagg ctccacagga aggtcgagga cagcgctccc ctatctccgg
    14281 cgccgggtcg gcacgaggcg caggcgatcc ggcgactcct cgacctcgac ggcggctttc
    14341 aggccggcca tgccgagcac cgcttccgtc ggcgcatcga cgatccggaa tgcaacctcc
    14401 ggcaggcgca ggtgctggag ctcgaccgca gccgcgagcc atcgctcgtc ctcgacgacg
    14461 gcacccgccg gcaggtcggc ggcagccagc cgggcttcgg cgccagtcgg atcgatgcgc
    14521 gcatggagcg ccgcgccggc gacgaggatg tcggcataga aggcgccgtc gccggcccga
    14581 tgcaggacga ccggccaacc cgcgtcgcga ccggccggac gggttccgag gagcgaggcg
    14641 gggaggacgt cgccgagcca gccggcggcg ctgtcgccac caagcgccgg ctcgatctcc
    14701 gcgggacgac ccggggtcga aggctccccc gccatccggc tggtctcgac ggccgggcgg
    14761 ccgagctggg ccagccatgc cgcggtcagg ccaaggacga gggccatcgg cagcaggagc
    14821 caggccggac ggcaggcggc tgccgcgcgc cggccggctt cggcgcgggc cgcaccgatc
    14881 gggtcgtgca gtttgacggc gaaacgctct gctggccgaa gcatagccga attccttacc
    14941 acgccattaa cgtgacgcat cagacccgaa gaatcttaag gaattctgaa ctttttcggt
    15001 gaatcggccg gtaagcagcc gaacggtgcc gcgccggccg ttgcatttca ggggcgctcg
    15061 ccacgtccga gccgcttctc gatcgccgcg atgcagggca gcagatcggc gacgctgtcg
    15121 acgacgtaat gggccccggc ctcggcgagc cgcgccacgg cccgctcacg ccttttttcc
    15181 tgctcggccg ggcgcagcgc ctgccagtcc gcatgggaga gcccgacctc gttgccgctg
    15241 atggcgaggc ccaccgtcca catcccggcg gccaggccct ccaggatgcc cggcaccgta
    15301 tcgtccacct tgacgcaggc cgcgacatcc gcgaccgcga gctcggcggc gttcttcagg
    15361 cacatcgccg ggcaggggcg ggcacgaggg acgtccgagg cggcgaccgc gctctgccag
    15421 gcgaagccct gccgcttcgc ctcggcgagg cagacctcca gcatgtcccg cgagtacccg
    15481 ctgttgacac cgatcccgat gccgcgctcg gcgagcgcct ggcccacatc gagggcaccc
    15541 ggcacgagcc cggcgaaagc cggcaggctc tcgatctgca aggggatgaa cgccgcatag
    15601 agctcgtcga cgtcctgctg gctccaggcc cggccgtggg ccgccgcgaa ccgctcggcg
    15661 acggcaggca gctcgagcag catctcgatg tggcggcgtt tctcggcacc catggggccg
    15721 cgcgcctcgg cgacggtcat ggccacccgg cggcgcctga acacctcgac gaagacgccg
    15781 gccggcgctc tggagccgaa gtccatgagc gtgccggcca tgtcgaagat caccgcacgg
    15841 accggtccga catagcggcg ctgccagcga taggcgtaca tgtcgctctt cctcgcttct
    15901 tctctgttgc ggctcagggt cggctgttcg ccacgccgag ctcgccgagc gtcgcctcga
    15961 tagccgccat ggccgcgtgc atgtcgtcgg caccgagggc accgatgcag ccgatgcgaa
    16021 agctgggcgc cacggtgagc ttgccgggat agatgacgaa gcccttcgcg gccaggcgat
    16081 cgtagaactg caggaagtcg aaagccgggt cccggggcat gcggaaggtg acgatgatcg
    16141 gcgcctgcaa cgcttcctcg aggagcggct ggaagccgag ccgccgcatg ccgtcgacca
    16201 ggacgcggca gttctcccga tagcgccggc cgcggccctc gaccccaccc tcctcctcat
    16261 gctggaccag cgcctcgtgg aacgcggcga ggacatgggt gggcggggtg aagcgccact
    16321 ggccggtcct gaccatggcc gcgtgctggg cggcgaggtc gagggtgagc gagtgggcgt
    16381 tgccttcggc cgcggccagg gcgtccttgc gggccagcac gaagcccata cctggcacgc
    16441 cttcgaggca cttgttggcc gaggccgcca cggcctcaaa gcggatcctc gcggcgtcga
    16501 gcggcagggc gccgaaggcg ctcatggcgt cgatcagcag ggcacggcca tgccgggcgg
    16561 caatcctggc gatctcgtcg atcgggttca ggatgccgga cgtcgtctcg cagtagacgg
    16621 cggccacatg gccgatcgcg ggatcggcgg ccagcgtctc ctccaggcgg cgcgtgtcgc
    16681 cgggacggtc ctcggggtcc tcgatggcga cgaccgcccg gcccatgtac cgtgccatct
    16741 cgaccatgcg cgcgccatag gcgccgttga ccatgaccag gagcttgccg tcgcgcggga
    16801 ccagcgtgcc cagcatggcc tccaccgcga agctgcccga gccctgcatg ggcaccgcaa
    16861 cgtgctcgtc gcccccaccg atcagctcga ccaggcgatc caggaccgca cggttgagcg
    16921 cgatgaagtc cgcgttgcgg gagccccagt cacggagcat cgcccgcttc acacgctccg
    16981 tcgtggtcag ggggcctggc gtgagcagcc gttggtgctg ggtcatcgag cacatccatg
    17041 ggcgccgggg cggtctcggc cgggcgcggt catcatcgcc ggtgccggac gatactgcgt
    17101 cgctcggccg cttgccaccg gttccggccc cgtgctgccg gtcaggcgcc gggcggcgcc
    17161 gtctcgaccg gtcgctcgaa gacgagcgag acacgcaggc cgggctcgtt gtccgcgagc
    17221 tcgagacggg cgtcgtgcag cttggcgacc gcgctcacca ggctgaggcc gagcccattg
    17281 ccgggcgtgt ggcgctcggg ctcgagcctg acgaagcgct cgagggcctt ctggcgcatt
    17341 tccggcggga tgcccggccc gtcgtcgctg accgttatcc tggcgatccg ccgagcctgc
    17401 tcccgcgtct gggcggcggc cagggtgacg tggccaccct cgggtgtgta cttgatggca
    17461 ttgtcggtca ggttggccag ggcctgggcg agcatctggc gattgcccgc gacggtgatg
    17521 ccggcggcgt tctcgagcgc aaggccgatg tcgcgctcct ccgccagcgg ctcgtagaga
    17581 tcgaagatgt cctgcacgag ctcgcccaga tcgac
//
LOCUS       DAOTFI010000099        17493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3912_length_17493_cov_6.081259, whole genome shotgun sequence.
ACCESSION   DAOTFI010000099 DAOTFI010000000
VERSION     DAOTFI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17493
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_17493_cov_6.081259"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1002)
                     /locus_tag="P5558_11610"
     CDS             complement(130..1002)
                     /locus_tag="P5558_11610"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY25002.1"
                     /translation="MRDLKGICVPICTPFRDGGAALDVAALEANIDSLIECGVHIIAV
                     NGGTGEFPFLAEAEKRQIAEIAAKRIDGRVGLVVQTSAIRTEDAIENSRHAEGLGADA
                     VLVLPPYFEGPGEDGVRWHYEQIARAIETPIMAYNIPVFTQFDITPEVFARFSEIDGL
                     AYLKDSTANPARIEQLIGQGSKVFCGCDFLNFYSLINGATGLFSGSGNAVPAELRRLW
                     DLVRADSLAEAAVLWKKLQPLSRLLWTLPFHPVAKAASGLTGRPAGLCRRPVPPLSAD
                     EMKLVEKAVAALAT"
     gene            complement(1058..1489)
                     /locus_tag="P5558_11615"
     CDS             complement(1058..1489)
                     /locus_tag="P5558_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HRY25003.1"
                     /translation="MLTLIAEILVALLIVIGSTFALIGSFGLIKLPDLATRLHGPTKA
                     TTLGVGAVLIASMIYFTVLEGDFSFHELVITLFLFLTAPLTANFIAKAYLHRHIRHDD
                     LPSTNREEGWATYDPPGEEGPLAPTQNPVTEPPARTPAGGA"
     gene            complement(1497..1766)
                     /locus_tag="P5558_11620"
     CDS             complement(1497..1766)
                     /locus_tag="P5558_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HRY25004.1"
                     /translation="MIDLALLYGFIALALALLCTAWRLSFGPTMADRILALDTMVIDT
                     LALIVLYGIHHGRSVLFEAAILIAMVGFISTVAYCRFILRGDVIE"
     gene            complement(1763..2260)
                     /locus_tag="P5558_11625"
     CDS             complement(1763..2260)
                     /locus_tag="P5558_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRY25005.1"
                     /translation="MKLHGRWLRHPLITLVLAGFWLLLMNEITLGGVLMGLFLGVVIP
                     LYTSRFWPEAPAIRHLPRVVEYTLIVLWDIVVANVQVAWWILFWKVERLRPSFMTVPL
                     DIYSPEAITMLAGTITMTPGTVSCDLSADGRALLVHGLHAPDVEETVAGIKDRYERRL
                     REIFG"
     gene            complement(2257..3822)
                     /locus_tag="P5558_11630"
     CDS             complement(2257..3822)
                     /locus_tag="P5558_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HRY25006.1"
                     /translation="MSNIIALPIVLPSLMAAVMVLAMRHDLVLQRIFGIASVSVVLAV
                     AIYILTRAANGEVMVYELGAWPAPFGIVLAIDRLAAMMLLLTSVVALLVLVYASAGDD
                     TRGRHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVLLIASYGLMLHGVGPRRLRAGTH
                     YVVVNLVGSTLFLFAVAFIYGVTGTLNMADLAVKVADVPAGDQAILQLGALLLLVVFG
                     VKAALVPLHFWLPATYASTSPPVAALFAIMTKVGAYAIIRVYISIFGAGAGDAAWVAA
                     PWLLPAALVTLVLGMVGVLAARSLRWMICFSVIGSMGMLFVAIGIFDPVATAAGLYYL
                     LHSTLIAAALFLLVDQIALRRGQTDDRLELAPVPAQGDLLAGLFFIGAIAFCGLPPLS
                     GFIGKLLILDAVQLDPRWPWIWALVLATSLLAITAFSRAGSLVFWKSRTVAVEDPKDA
                     EAEPPSGPAPARPVPLSVVALFLLGTVLLTAFAAPIGRYVDATAAQLHDPVDYIEAVL
                     GVATLAPTEEVAE"
     gene            complement(3819..4181)
                     /locus_tag="P5558_11635"
     CDS             complement(3819..4181)
                     /locus_tag="P5558_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HRY25007.1"
                     /translation="MELLISSGVGVLVASGVYLVLRARTFPVIVGLALLSYAVNLLIF
                     GMGRLVIDRPPILSLHLDAYTDPLPQALVLTAIVIAFGMTALIVVIILRSFLETGSDH
                     VDGDEEGRLTELDERSGR"
     gene            complement(4192..7053)
                     /locus_tag="P5558_11640"
     CDS             complement(4192..7053)
                     /locus_tag="P5558_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HRY25008.1"
                     /translation="MQLALIVLLPFVGALLPPLLIRAGRNTCATATFLVTLTALLVLL
                     VQAPAVLGGEILQFRLEWLPLLGLDVNFFLDGYGFLFAGLILFIGLLIIVYARFYLSR
                     EDPMGRFMAYLLLFQGAMVGIVLSDNILLLLIFWELTSLSSFLLIGYWNHLPEGRQGA
                     RMALIVTGSGGLSMIAGMLLLGQIAGSYDLTVILSQGELVKNSPLYLPMLLLILGGAF
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGIFLLGRLWPALAGTEEWFYLVAT
                     TGLVTMAVAAWIALFKDDLKALLAFSTVSHLGLVTMLFGFGTPFAAMAGVLHIINHAT
                     FKCALFMTAGIVDHETHTRDIRRLGGLMMLMPVTGTIAVVAAFSMAGMPPLNGFLSKE
                     LMLEAAAHTDWLGSHLVVGILATVAALFSAAYSFRYIFHVFIGRQRDDYPHHPHDPPF
                     GMWAPPAILVLLVVLIGFFPNTIVGPLLAVSAGAVTGGRLPDYSLALWHGITPALLMS
                     LVATVGGALLLWQHRAGLRLRSCFWRPEAKAIFEGLIELVVAWSRAGTTALQNGSLQR
                     YLAITISAAIVIGYTGFLGSSLEPGTRAPLHAPWIAVSTWLVLVVACVAVVVLHRRRL
                     FSLIVVGVLGLIVSLAFLYLSAPDLALTQISVEVVTVILMLLALNFLPKETRPESGPG
                     RRLRDGIIAGLAGLGIAAAMHAVLTRDFSTISDYHLANSKTGGGGTNVVNVILVDFRG
                     FDTYGEIIVLGIAALTIFALLDTALQGACGRRLMAWQADQARSADRHPMMMVVGTRAM
                     LPLALMVGVYIFLRGHNEPGGGFIAGLVVAIALLMQYMASGFAWAQERARVDYHAMIA
                     TGVLAAGLTGIAAWFFGWPFLKSTYTYLHVPLIGEVEVASAMGFDLGVFLCVVGSVML
                     ALANLSRIGRIAEHLEVPEGPMDVDPSKLIDPAVVPAAN"
     gene            complement(7181..7963)
                     /locus_tag="P5558_11645"
     CDS             complement(7181..7963)
                     /locus_tag="P5558_11645"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRY25009.1"
                     /translation="MRSIAALALCPLLYGLPATAANLEAGYSVRWAGIEIARFEVELR
                     TSPADYRLAYRAETTGALAWVFDFASDGSATGRLTPEGPIAGQYRGRSASDDHTSQWR
                     VDFTADGAVSEVVLDEESRGDREPVPAELMVAPDPFTLALQGLLEAAAGKRLEGRSFD
                     GKRVMGFTMDCAPAPGPVHPAAFPAPRGGALFCTADGELLAGRSKRWGDRTDEHNEGR
                     PPARLWLAPGVGGLPHWPVRIEAEGRFGAVSIELDHLVEPAA"
     gene            8072..9055
                     /locus_tag="P5558_11650"
     CDS             8072..9055
                     /locus_tag="P5558_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRY25010.1"
                     /translation="MRAMVLTRPGEPLELCRLSRPAPGPGQVLIEVAACGVCRTDLHV
                     VDGELTRPKLPLVPGHEIVGRVAALGSGVAGLEVGQRVGVPWLGHTCGCCPYCRRGAE
                     NLCDAPGFTGYTIDGGYAEACVADAAYVFPLPEGPGDAELAPLLCAGLIGYRSLVMAG
                     EARVIGIYGFGAAAHILAQVMRHQGRRFLAFTSPGDAAAQAFARRLGAAWAGGSDEVS
                     PEPLDAAILFAPVGALVPAALRAVRKGGVVVCGGIHMSDIPSFPYDILWGERRVVSVA
                     NLTRRDGEEFLALAAEAGVETEVTPYPLERANEALADLRRGALTGAAVLVP"
     gene            9150..10310
                     /locus_tag="P5558_11655"
     CDS             9150..10310
                     /locus_tag="P5558_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25011.1"
                     /translation="MRFNHGLIGGVAALGLLAGAAAAEPVKVGMVTTLSTGGGYLGQD
                     VRDGFILALEMAGATDRVELLVEDDGLEPENGVQIAERMVERDGAEVMTGIIFSNVAM
                     AVVPKLVRGGMIYLSPNAGPSALAGKGCHENYFNVAWQNDDLHAAMGAYVAGQGFDNV
                     YLLAPNYPAGKDALTGFKRFYKGNVAGEVYTQLGQTDYAAELANLRAAGPDAVFFFYP
                     GGMGINFVKQAAQAGISQEIPFFGPAFSFDDTLLDAVGDAAVGIKNTSQWSPDLDNEA
                     NKAFVAAFKDKYGRYPTLYASQGFDTANLLLSAMDAVGDDVSDQAAFREALRAADFVS
                     VRGDFRFGPNQHPIQDIYVREAYVDDSGNVTNRIVGMVLEDHADAYAAECAM"
     gene            10344..11252
                     /locus_tag="P5558_11660"
     CDS             10344..11252
                     /locus_tag="P5558_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY25012.1"
                     /translation="MLLFLEQLLNGVQLGMTLFMMAAGLTLVFGVMNFINLAHGSLFM
                     AGAYVAAAVVAATGSFLLGLVAGVAVAALFGVVLDRVIVRPLAGRGHLDQVLATFAVI
                     LIANETVRLVFGRQPLALDPPGWLAGTVEILPGVPYPLYRLAVILVGLLVMAGLWLLV
                     MRTRVGMWVRAGATHPEMIQLLGVDIGRLNGLIFAAGAALAGLAGIVAGPFIAIQSGM
                     GEAILILAFVVVVIGGIGSVLGAAIGALLVGVGDTLGRAYLPALVHELAPAGTAEGLG
                     PALASMGIYLLMALVLALRPKGLLGR"
     gene            11249..12211
                     /locus_tag="P5558_11665"
     CDS             11249..12211
                     /locus_tag="P5558_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY25013.1"
                     /translation="MSRAWPLLLVMGALALAGLYVELSGERYLWTVLTKIAIYGLAAS
                     GLNLVLGVGGLVSFGHAAFLGLGAYVVGILSLHAFEEAPLMGLFASNAALVAWPLAVA
                     TGAIAGLLIGLVSLRTGGAYFIMITLAFAQMLYFGFVSAERYGGEDGLSLWWGRNELL
                     GLSLDDRRAFFIVVWLILGGWSVLKLRLMAAPFGRALSASRMNPERLAALGVDVFRLR
                     LVAFAVSAAVTALAGALLANLTGFVSPASLAWPVSGELIVMCVLGGLGTVAGPILGAA
                     ALILLEETLVSFTEHWQLFVGLILLAIVLASRRGVSGLLGLRRG"
     gene            12204..12938
                     /locus_tag="P5558_11670"
     CDS             12204..12938
                     /locus_tag="P5558_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25014.1"
                     /translation="MAERPLLEASGLAKAYGGVFAVQGVSLDLVAGEIHALIGPNGAG
                     KSTLIGLLHGAIPADAGTVAFAGSTLDGRGPAGRARAGLARSFQRTALFAELSVAEHV
                     GLAIRAAGGRSFGWWREARHPELAVLDELGLGDVAHRPAGELAHGRQRLVELAMVVAA
                     RPRAILMDEPFAGLAPDDRPALARRLAALKSRHALLLVEHDMDVVFQLADRISVLVAG
                     RLIRSDTPEAIRADPEVQRAYLRDED"
     gene            12938..13624
                     /locus_tag="P5558_11675"
     CDS             12938..13624
                     /locus_tag="P5558_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25015.1"
                     /translation="MLAVHGLEAAYGATQVLFGVSLEVGEGRIVGLLGRNGMGKTTLI
                     RSLYNLTRRLGGSVRFEGEDLDPLPPFAIARRGIAVVPEGRQIFPGLSVEENLVATAR
                     PGEWRLGRVLDLFPRLAERRRNPGGSLSGGEQQMLAIGRALLTNPRLLVLDEATEGLA
                     PAVRREIWQCIRALKATGIAILLVDPHLGRLLELTDTNVIIENGRSVWQGSSAELADR
                     PALQRRYLAV"
     gene            complement(13649..14116)
                     /locus_tag="P5558_11680"
     CDS             complement(13649..14116)
                     /locus_tag="P5558_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25016.1"
                     /translation="MNVINNSTLLVRRISDLQRRRDILVERQDQLRRTLPEWAFAPLQ
                     LAGMSSAEIRGMMQEMSRVESDAGLDAIERELEQLDQRIEELENDLLATPARSLDSIH
                     AVLELALGRVRGQTSKDPNDLFYDYGDARVLSFLERAADDMRSLLQEAHREAV"
     gene            14426..15157
                     /locus_tag="P5558_11685"
     CDS             14426..15157
                     /locus_tag="P5558_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRY25017.1"
                     /translation="MAGSRDGRRRPAGEGGGEGVARSPSLLARLRTYLFAGIVVTAPV
                     SITFFIVWQILDFLDRTASRLIPDRYDPASYLPFGIPGLGLLVMLLLLIFIGWFAAGF
                     VGRSLMRAGERVLDRMPVVRSIYGTLKQIFETVLAQSSRSFREVVLLEWPRRGLWVIG
                     FVTGPTSGEIKSRFDGDFVNIFLPTTPNPTSGFLVFVPARDLIHLDMSVEEGIKLVIS
                     GGIVAPARDRGLMAGGAEARPPAGT"
     gene            15219..16073
                     /locus_tag="P5558_11690"
     CDS             15219..16073
                     /locus_tag="P5558_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY25018.1"
                     /translation="MPDVDATNLLARIAEALERQSPPPALSLGLGAARAYVFDAPARA
                     LRPVPRVARIDFELLCGIDHVAQILLDNTRRFARGLPANNALLWGARGMGKSSLVKAV
                     HGLVEREGADLILIEIHREDIDSLPHLLHDLARQPDRFLLFCDDLSFDAGESSYKSLK
                     AVLEGGLEGRPQNVLFYATSNRRHLMPRQMIDNERGTAINPGEAVEEKVSLSDRFGLW
                     LGFHPCNQETYLAMVDRYAGHFRLEVEPGELKRQALEWSTTRGSRSGRVAFQFIQDLA
                     GRLGKRLG"
     gene            complement(16078..>17493)
                     /locus_tag="P5558_11695"
     CDS             complement(16078..>17493)
                     /locus_tag="P5558_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY25019.1"
                     /translation="IVFIITDQQRHDTIKALGHDHMITPNLDRMVEEGVAFTRMYCTS
                     PVCSPSRASLFSGLYPHSSGVLRNDERWPYTWVHLLAEAGYRCVNVGKMHTHPFEEPF
                     GFHERHVTENKDRAHPALPFYLDNWDKALWAAGHTKPSRQTYCHRADYKDRLGAFVWE
                     LPEKLHPDVFVAQSAVHWLESYKGEEPFFLQVGIPGPHPPYDPTAEALALYDGVAFPQ
                     PIRNYDLDSQPTAMRRLRQNHIANDHDAVVHLADPTPEQMQRQRAHYAANVTMIDEQL
                     GHLKAALEARGVLDDTVIIFTSDHGDSLNDHGHSQKWSMFESSVRVPCVMHDPRRQDG
                     GRRIDDLTSLFDLGPTILELAGVAPPAWMEARSLVPYFDRDTDPARDTVYAELADDMI
                     QEDCPFVTMIRTGKWKLVHYLGSSEGQLFDLEADPDEVENLWDAPAHQATRQELTHRI
                     LEWRLQSARRTQKFLVEGLTS"
BASE COUNT         2648 a         5685 c         6501 g         2659 t
ORIGIN      
        1 ccgagatcgc ggtcgtcgag cggaccggct cgggccgtga ggcgctggct ctgacagcgg
       61 gtctctgccg cgtgctcgac aggcccgccg cggtagcagt agtcgggctt cgtcccgttg
      121 cccggcctct cacgtcgcaa gggcggcgac cgccttctcc accagcttca tctcgtcggc
      181 cgagaggggt ggcaccggcc tgcggcagag gccggccggg cggccggtca gcccgctcgc
      241 ggccttggcg accggatgga acggcagggt ccagagcagc cggctgagcg gctggagctt
      301 cttccacagg acagccgcct cggcgaggct gtcggcccgg acgaggtccc aaaggcgcct
      361 gagctcggcc gggacggcat tgccggatcc gctgaacagc ccggtggcgc cgttgatcag
      421 gctgtagaaa ttgaggaagt cgcagccgca gaagaccttc gagccctgcc cgatcaattg
      481 ctcgatgcgg gccggattgg cggtgctgtc cttgaggtag gcgaggccgt cgatttccga
      541 gaagcgggcg aagacctcgg gcgtgatgtc gaactgggtg aagaccggga tgttgtaggc
      601 catgatcggc gtctcgatag cccttgcgat ctgctcgtag tgccaccgca cgccatcctc
      661 gcccgggccc tcgaaatagg gtggcagaac gaggacggcg tcggcgccca ggccctcggc
      721 gtgcctggag ttctcgatcg cgtcctcggt gcggatggcc gacgtctgga cgacaagccc
      781 gacgcgaccg tcgatgcgct tcgcggcgat ctcggcgatc tggcgcttct ccgcttccgc
      841 gaggaaggga aattcgccgg tgccgccgtt gacggcgatg atgtggacgc cgcattcgat
      901 cagcgagtcg atattggctt cgagggcggc cacgtccaat gccgcgccgc cgtccctgaa
      961 gggggtgcag atcggcacgc agatgccttt gaggtcgcgc atcgggttct cctcggtggg
     1021 gcggtcgccc cgtcgtgcgg gggcttgcga ccgctggtca agctcctccc gcgggcgtcc
     1081 tcgccggtgg ctcggtgacc gggttctggg tcggggcgag ggggccttcc tcgcccggcg
     1141 ggtcgtaggt cgcccagcct tcctcgcggt tggtcgaggg caggtcgtcg tggcggatgt
     1201 ggcggtgcag gtaggccttg gcgatgaagt tggccgtcag cggcgcggtc aggaagagga
     1261 agagggtgat gaccagctcg tggaacgaga agtcgccctc gagcacggtg aagtagatca
     1321 tggacgcgat cagcacggcg ccgacgccga gcgtcgtggc cttggtcggc ccgtgcaggc
     1381 gggtggcgag gtccggcagc ttgatcagcc cgaaggagcc gatgagcgcg aaggtgctgc
     1441 cgatgacgat cagcaatgcc acgaggatct cggcgatgag ggtcagcatg gccggcctat
     1501 tcgatcacgt cgccgcggag gatgaagcgg cagtaggcca cggtcgagat gaagccgacc
     1561 atggcgatca ggatggcggc ctcgaacagc accgagcggc cgtggtggat gccgtagagc
     1621 acgatgagcg ccagggtgtc gatcaccatg gtgtcgaggg cgaggatgcg atccgccatg
     1681 gtcggcccga acgagaggcg ccaggcggtg cagagcagcg ccagcgccag ggcgatgaag
     1741 ccgtagagga gggcgaggtc gatcatccga agatctcccg gagccggcgc tcgtagcggt
     1801 ccttgatgcc ggcgaccgtc tcctcgacgt cgggcgcgtg aaggccgtgg acgagcaggg
     1861 cccggccgtc cgccgagagg tcgcaggaaa cggtgccggg cgtcatggtg atggtcccgg
     1921 cgagcatggt gatggcctcg ggcgagtaga tgtcgagcgg cacggtcatg aagctaggcc
     1981 gcagccgctc gaccttccag aagaggatcc accaggcaac ctggacattg gcgacgacga
     2041 tgtcccagag gacgatcagg gtgtactcga cgaccctggg caggtggcgg atcgccggcg
     2101 cctcgggcca gaaccggctg gtgtagagcg ggatcacgac gccgaggaaa aggcccatga
     2161 ggacgccgcc gagcgtgatc tcgttcatca ggagcagcca gaagccggcc agcaccaggg
     2221 tgatcagggg gtgtcgcagc cagcggccat ggagcttcat tccgccacct cctccgtcgg
     2281 ggccagcgtg gcgacgccga gcacggcctc gatgtagtcc accgggtcgt ggagctgggc
     2341 cgcggtggcg tcgacatagc ggccgatggg ggccgcgaac gcggtgagca ggacggtgcc
     2401 gaggaggaag agggcgacca cgctgagcgg cacgggccgg gccggcgccg gccccgaggg
     2461 gggctcggcc tcggcatcct ttgggtcctc gaccgccacg gtccggctct tccagaagac
     2521 caggctgccg gcgcgggaga aggcggtgat ggcgagcagc gaggttgcga ggacgagcgc
     2581 ccagatccag ggccagcgcg ggtcgagctg cacggcgtcc aggatcagga gcttgccgat
     2641 gaagcccgag agcggcggca gtccgcagaa ggcgatggcg ccgatgaaga agaggccggc
     2701 caggaggtcg ccctgcgccg ggaccggggc gagctccagc cggtcgtcgg tctgcccccg
     2761 gcggagcgcg atctggtcga ccagcaggaa gagggcggcg gcgatcaggg tcgagtgcag
     2821 caggtagtag agcccggccg cggtggcgac ggggtcgaag atgccgatgg cgacaaagag
     2881 catgcccatc gagccgatga ccgagaagca gatcatccag cgcagcgagc gggcggcgag
     2941 caccccgacc atgccgagca cgagcgtgac gagggcggcg ggcagcagcc agggggcggc
     3001 gacccaggcg gcgtcgccgg cgccggcgcc gaagatcgag atgtagaccc ggatgatggc
     3061 gtaggcgccg accttggtca tgatggcgaa gagggccgcc accggcggcg aggtcgaggc
     3121 ataggtggcg ggcagccaga aatgcaaggg caccagggcc gccttgacgc cgaagacgac
     3181 caggaggagg agggcgccga gctgcaggat ggcctggtcg ccggccggca cgtccgccac
     3241 cttgaccgcg aggtcggcca tgttgagcgt gccggtgacg ccgtagatga aggccacggc
     3301 gaagaggaag agggtcgagc ccacgaggtt gacgacgacg tagtgggtgc cggcccggag
     3361 ccggcgcggg ccgacgccgt gcagcatcag gccataggag gcgatgagca gcacctcgaa
     3421 gaagacgaag aggttgaaga tgtcgccggt gaggaaggcg ccgttgagcc ccatgagctg
     3481 gaactggaag agcgcgtgga agtggcggcc gcgggtgtcg tcgccggcgc tggcatagac
     3541 gaggacgagc agcgccacga ctgaggtcag gagcagcatc atggcggcga gccggtcgat
     3601 cgccagcacg atgccgaagg gtgccggcca ggcgccgagc tcgtagacca tgacctcgcc
     3661 attggcggcg cgggtcagga tgtagatggc gaccgcgagg acgaccgaga ccgaggcgat
     3721 gccgaagatc cgctgcagca cgaggtcgtg gcgcatcgcc agcaccatga cggcggccat
     3781 gagcgacggc aggacgatcg gcagggcgat gatgttgctc aacggccgct ccgctcatcg
     3841 agctcggtca gccggccttc ctcgtcgccg tcgacatggt cgctgccggt ctcgaggaag
     3901 ctgcgcagga tgatgacgac gatgagcgcc gtcatgccga aggcgatgac gatggcggtg
     3961 agcaccagcg cctgcggcag ggggtcggtg taggcgtcga gatggaggct gaggatgggc
     4021 gggcggtcga tgacgaggcg gcccatgccg aagatcagga ggttgaccgc gtaggagagc
     4081 aaggcgaggc cgacgatcac cggaaaggtg cgcgctctca gcacgagata gacgccggaa
     4141 gcgaccagga caccgactcc gctggagatc aggagctcca tggacgggct cctagttcgc
     4201 tgccggcacg acggccgggt cgatgagctt cgagggatcg acgtccatgg gcccttccgg
     4261 cacctcgaga tgctcggcga tccggccgat gcgcgagaga ttggcgagcg ccagcatcac
     4321 cgagccgacg acgcagagga agacgccgag gtcgaagccc atggccgagg cgacctcgac
     4381 ctcgccgatg agcggcacgt ggagataggt gtaggtgctc ttgaggaagg gccagccgaa
     4441 gaaccaggcg gcgatgccgg tgagcccggc ggcgagcacg cccgtggcga tcatcgcgtg
     4501 gtagtcgacc cgtgcgcgct cctgcgccca ggcgaagccg ctcgccatgt actgcatgag
     4561 cagggcgatg gcgacgacca gcccggcgat gaagccgccg ccgggctcgt tgtggccgcg
     4621 caggaagatg tagacgccga ccatcagcgc cagcggcagc atggcgcggg tgccgaccac
     4681 catcatcatc gggtgccggt cggcggagcg ggcctggtcg gcctgccacg ccatcaggcg
     4741 gcggccgcag gcgccctgca gggccgtgtc gagcagggcg aagatggtca gcgccgcgat
     4801 gccgagcacg atgatctcgc cataggtgtc gaagccgcgg aagtcgacga ggatgacgtt
     4861 gacgacattg gtgccaccgc cgccggtctt cgagttggcg aggtgatagt ccgagatcgt
     4921 ggagaaatcg cgggtcagca ctgcatgcat ggcggcggcg atgccgagcc cggcgaggcc
     4981 ggcgatgatg ccgtcgcgca ggcggcggcc cggcccgctt tccggccgcg tctccttggg
     5041 caggaagttg agcgccagca gcatgaggat gacggtcacc acctcgaccg agatctgcgt
     5101 cagcgccagg tccggtgccg agaggtagag gaaggcgagg ctgacgatca ggccgagcac
     5161 gccgacgacg atcagcgaga agagccggcg ccggtgcagg acgacgacgg cgacacaggc
     5221 gacgacgagc acgagccagg tgctcacggc gatccagggg gcgtgcaggg gggcgcgcgt
     5281 gccgggctcg agcgagctgc cgaggaagcc cgtgtagccg atgacgatgg cggccgagat
     5341 ggtgatggcg agatagcgct gcagcgagcc gttctgcagc gccgtggtgc ccgcccggct
     5401 ccaggcgacg acgagctcga tcaggccctc gaagatcgcc ttggcctcgg gccgccagaa
     5461 gcacgagcgc aggcgcagcc cggcccggtg ctgccagagc aggagggcgc cgccgacggt
     5521 ggcgacgagg ctcatgagga gggccggggt gatgccgtgc cagagcgcca gcgagtagtc
     5581 gggcagccgg ccgccggtca ccgcacccgc cgagacggcg agcagcggcc ccacgatggt
     5641 gtttgggaag aagccgatca gcacgaccag caggacgagg atcgccggcg gcgcccacat
     5701 gccgaagggc gggtcgtgcg ggtggtgcgg atagtcgtcg cgctgccggc cgatgaagac
     5761 gtggaagata tagcggaacg aataggccgc cgagaagagg gccgccaccg tggcgaggat
     5821 gccgaccacg agatggctgc cgagccagtc ggtgtgggcc gcggcctcca gcatgagctc
     5881 cttcgaaagg aagccgttga gcggcggcat gccggccatc gagaaggccg cgacgacggc
     5941 gatcgtgccg gtcaccggca tcagcatcat gagcccgccg aggcggcgga tgtcgcgcgt
     6001 gtgggtctcg tggtcgacga tcccggcggt catgaagagc gcgcacttga aggtggcgtg
     6061 gttgatgatg tgcagcacgc cggccatggc ggcgaagggt gtgccgaagc cgaagagcat
     6121 ggtgacgagg ccgagatggc tcaccgtgga gaaggcgagc agcgccttga ggtcgtcctt
     6181 gaagagcgcg atccaggcgg cgacggccat ggtgacgagg ccggtcgtgg cgaccaggta
     6241 gaaccactcc tcggtgccgg ccagcgccgg ccagaggcgg ccgaggagga agatcccggc
     6301 cttcaccatg gtggcgctgt gcagataggc gctgaccggg gtgggtgctg ccatggcgtg
     6361 cggcagccag aagtggaacg ggaactgcgc cgacttggtg aaggcgccgc cgagaatgag
     6421 cagcagcatc ggcagataga gcggcgaatt cttgacgagc tcaccctggc tcaggatgac
     6481 ggtgagatcg taggagccgg cgatctggcc gagcagcagc atgccggcga tcatcgagag
     6541 cccgcccgag cccgtgacga tgagcgccat ccgggcgccc tggcggcctt ccggcaggtg
     6601 gttccagtag ccgatcagga ggaacgagga gaggctggtc agctcccaga agatgagcag
     6661 gaggaggatg ttgtcggaga gcacgatgcc gaccatggcc ccctggaaga gcaggagata
     6721 ggccatgaag cggcccatcg gatcctcgcg ggagagatag aaccgcgcat agacgatgat
     6781 cagaaggccg atgaacagga tcagcccggc gaagaggaag ccgtagccgt cgaggaagaa
     6841 gttcacgtcg aggccgagca gcggcagcca ctcgagacgg aactgcagga tctcgccgcc
     6901 gaggacggcg ggcgcctgga ccaggagcac gagcagggcc gtcagggtga cgaggaaggt
     6961 ggcggtggcg caggtgttgc gccccgcgcg gatcagcaag ggcggcagga gcgcgccgac
     7021 gaacggcagc aggacgatga gggccagttg catcgggtct ctcggcagtg cctccccggg
     7081 cccgctcgcg tcgtccgcgt cgcgaccccc gggccttgaa ccacacgggc cggaaggtca
     7141 gcaagcgcaa tcggaacgtg gcggggaacg ggccggcggc tcaggccgcc ggctcgacca
     7201 ggtgatcgag ctcgatggag acggcgccga agcggccttc cgcctcgatc cggaccggcc
     7261 agtgcggcag gccgccgaca cccggcgcca gccagaggcg ggccggcggg cggccctcgt
     7321 tgtgctcgtc ggttcgatca ccccagcgct tcgagcggcc ggcgaggagc tcgccgtcgg
     7381 cggtgcagaa gagcgccccg ccgcgcggtg ccgggaaggc cgccggatgg acggggccgg
     7441 gcgccggcgc gcaatccatg gtgaagccca tgacgcgctt gccgtcgaag ctccggccct
     7501 cgagccgctt gccggcggcg gcctcgagca gcccctggag cgccagggtg aaggggtcgg
     7561 gcgccaccat cagctcggcc gggacgggct cccggtcgcc ccggctttcc tcgtccagga
     7621 ccacctcgct gacggcacca tccgcggtga agtcgacccg ccattggctg gtgtggtcgt
     7681 cactggcgct gcggccccga tactgcccgg cgatcgggcc ctccggcgtc agccgacccg
     7741 tggccgagcc gtcgctggcg aagtcgaaga cccaggcgag ggccccggtg gtctcggcgc
     7801 gataggcgag gcggtagtcg gccggcgagg tgcgcagctc gacctcgaag cgggcgatct
     7861 cgatgccggc ccagcgcacg ctgtagcccg cctcgagatt cgccgccgtg gccggcaggc
     7921 cgtagaggag ggggcagagg gcgagggcgg cgatgctgcg catgggccga agatgcatcg
     7981 gcgcgcggcc gggtcaagca cgatgcgctt ggtcgcccgc aggttcgccg ctaggctcga
     8041 tgaacctgcc cgggaaaggg ggtttcgtgt gatgcgggcg atggtcctga cgcggcccgg
     8101 cgagcctttg gagttgtgcc ggctgtcgcg gccggcgccg ggccccgggc aggtgctgat
     8161 cgaggttgcc gcctgcgggg tctgccggac cgacctgcac gtcgtcgatg gcgagctgac
     8221 ccggcccaag ctgccgctgg tgcccgggca cgagatcgtc ggccgggtgg cggcgctggg
     8281 cagcggcgtc gccgggctcg aggtcgggca gcgggtgggg gtgccctggc tcggccacac
     8341 ctgcgggtgc tgcccctatt gccggcgggg tgccgagaac ctgtgcgatg cgccgggctt
     8401 caccggctac accatcgacg gcggctacgc cgaggcctgc gtggcggatg cggcctatgt
     8461 cttcccgctg ccggaggggc cgggcgatgc cgagctggcg ccgctgctct gcgccgggct
     8521 gatcggctac cgctcgctgg tcatggcggg cgaggcgcgg gtgatcggga tctatggctt
     8581 cggtgcggcg gcgcacatcc tggcgcaggt catgcgccac cagggccgcc gcttcctcgc
     8641 cttcaccagc cccggcgacg cggcggcgca ggccttcgcc cgccggctcg gcgccgcctg
     8701 ggccggtggc tccgacgagg tttcgcccga gccgctcgac gccgccatcc tcttcgcgcc
     8761 ggtcggcgcg ctcgtcccgg cggcgctccg ggcggtgagg aagggcgggg tcgtggtctg
     8821 cggcggcatc cacatgagcg acatcccgag cttcccttac gacattctct ggggcgagcg
     8881 gcgggtcgtc tcggtcgcca acctgacccg gcgcgacggc gaggagttcc tggcgctggc
     8941 cgcggaagcc ggggtcgaga ccgaggtgac gccgtacccg ctcgagcgtg ccaacgaggc
     9001 gctggcggat ctccgccggg gcgccctcac gggtgccgcg gtgctggtgc cctgaggcgg
     9061 ccgatcggcc cggccttgcc ttgccaccgg cttcgcgcta ccgccggggc atctggcgaa
     9121 tgcgaagagc aacgaagagc gagggtggca tgaggttcaa tcatgggctg atcggcggtg
     9181 ttgcggcact cggcctgctg gcgggtgctg cggcagccga gccggtcaag gtcggcatgg
     9241 tgacgacgct ttcgaccggc ggcggctatc tcggccagga cgtgcgcgac gggttcatcc
     9301 tggcgctgga gatggccggg gcgaccgatc gggtcgagct gctggtcgag gatgacgggc
     9361 tcgagcccga gaacggcgtg cagatcgccg agcgcatggt cgagcgcgac ggggcggagg
     9421 tgatgaccgg catcatcttc tccaacgtcg ccatggcggt ggtgccgaag ctggtccggg
     9481 gcgggatgat ctatctctcg cccaatgccg gcccgtcggc gctggccggc aagggctgcc
     9541 acgagaacta cttcaacgtc gcctggcaga acgacgacct gcacgccgcg atgggcgcct
     9601 acgtggccgg gcagggcttc gacaacgtct atctgctcgc ccccaactac ccggccggca
     9661 aggacgcgct gaccggcttc aagcggttct acaagggcaa tgtcgccggc gaggtctaca
     9721 cccagctcgg ccagaccgac tacgcggcgg agctcgccaa cctgcgggcg gcgggaccgg
     9781 acgcggtctt cttcttctac ccgggcggca tgggcatcaa cttcgtcaag caggcggcgc
     9841 aggccgggat cagccaggag atccccttct tcggcccggc cttctccttc gacgacacgc
     9901 tgctcgacgc ggtgggcgac gcggcggtcg gcatcaagaa cacgtcgcaa tggtcgccgg
     9961 acctcgacaa cgaggcgaac aaggccttcg tcgccgcctt caaggataaa tatggccgct
    10021 atcccacgct ctatgcgagc cagggcttcg acacggccaa cctgctgctc agcgccatgg
    10081 acgcggtcgg cgacgacgtc tcggaccagg ctgcgttccg cgaggcgctc agggccgccg
    10141 atttcgtctc ggtgcgcggc gatttccgct tcgggccgaa ccagcacccg atccaggaca
    10201 tctacgtgcg cgaggcctat gtcgacgaca gcggcaacgt caccaaccgc atcgtcggca
    10261 tggtgctcga ggaccacgcc gacgcctacg ccgcggaatg cgccatgtag ctcgcgcctc
    10321 ggcgaccgta ccggccgggc tgcatgctgc tctttctcga acagcttctg aacggcgtgc
    10381 agctcggcat gacgctcttc atgatggcgg ccgggttgac gctggtgttc ggcgtcatga
    10441 acttcatcaa cctggcgcat ggctcgctct tcatggcggg ggcgtatgtg gccgctgccg
    10501 tggtggctgc cacgggctcg ttcctgctcg ggctggtggc cggcgtcgcc gtcgccgcgc
    10561 tcttcggcgt cgtgctcgac cgggtgatcg tgcgtccgct ggcgggccgg gggcatctcg
    10621 accaggtgct cgccaccttc gcggtgatcc tgatcgccaa cgagacggtg cgcctcgtct
    10681 tcggtcggca gcccctggcc ctcgacccgc cgggctggct cgccggcacg gtggagatcc
    10741 tgcccggcgt cccctatccg ctctatcgcc tcgccgtgat cctggtcggc ctcctcgtca
    10801 tggccgggct ctggctgctc gtgatgcgca cgcgggtcgg catgtgggtc agggccgggg
    10861 ccacgcatcc cgagatgatc cagctcctgg gcgtcgacat cggccggctc aacgggctga
    10921 tcttcgcagc aggtgccgcg ctcgccgggc tcgccggcat cgtcgccggg cccttcatcg
    10981 ccatccagtc cggcatgggc gaggcgattc tcatcctcgc cttcgtcgtc gtcgtcatcg
    11041 gcggcatcgg ctcggtcctg ggagccgcca tcggtgccct gctggtcggg gtcggcgaca
    11101 cgctgggccg ggcgtacctt ccggccctgg tgcacgagct ggcgccggcc ggcacggcgg
    11161 aggggctcgg cccggcgctc gcctcgatgg gcatctacct cctgatggcg ctggtgctgg
    11221 cgcttcggcc caaggggctg ctcgggcgat gagccgggcc tggccgctcc tcctcgtcat
    11281 gggcgccctg gcgctcgccg ggctctatgt cgagctttcg ggcgagcgct atctctggac
    11341 cgtgctcacc aagatcgcga tctacggcct cgccgcctcg gggctcaacc tcgtcctggg
    11401 cgtgggcggg ctcgtcagct tcggccatgc ggcctttctc ggcctcggcg cgtatgtggt
    11461 cggcatcctc tcgctgcacg ccttcgagga ggcgccgctc atgggcctct tcgccagcaa
    11521 cgcggccctc gtcgcctggc cgctggcggt ggccacgggt gccatcgccg ggctcctgat
    11581 cggcctcgtc tcgctgcgca ccggcggcgc ctatttcatc atgatcacgc tggccttcgc
    11641 ccagatgctc tatttcggct tcgtctcggc cgagcgctat ggcggcgagg acggactctc
    11701 gctctggtgg gggcgaaacg agcttctggg cctctcgctc gacgaccggc gggccttctt
    11761 cattgtcgtc tggctgatcc tcggtggctg gagcgtgctc aagctgcgcc tgatggcggc
    11821 acctttcggc cgggcgctca gcgcgagccg gatgaacccg gagcggctcg cggcgctggg
    11881 cgtcgacgtc tttcgcctgc gcctcgtcgc cttcgcggtc tcggcggcgg tcaccgccct
    11941 cgccggggcg ctgctcgcca acctcaccgg cttcgtcagc ccggcgagcc tcgcctggcc
    12001 ggtctcgggc gagctcatcg tgatgtgcgt cctgggtggc ctcggcaccg tcgccgggcc
    12061 gatcctgggg gccgcggcgc tgatcctgct cgaggagacg ctggtctcct tcaccgagca
    12121 ctggcagctc ttcgtcggcc tcatcttgct cgccatcgtg ctggcctcgc gccgcggcgt
    12181 cagcggcctt ctgggtctcc gccgtggctg agcgccccct gctcgaggcg agcggcctcg
    12241 ccaaggccta tggcggcgtc ttcgccgtcc agggcgtgtc gctcgacctc gtggcgggcg
    12301 agatccatgc gctgatcggg ccgaacgggg ccggcaagag cacgctgatc ggcctcctgc
    12361 atggcgccat tccggccgat gccggcacgg tcgcgttcgc gggcagcacg ctcgacggcc
    12421 gggggccggc gggccgggcg cgggcggggc tcgcccgctc gttccagcgg acggcacttt
    12481 ttgccgagct ctcggttgcc gagcatgtcg gcctcgccat ccgggcggcg ggcgggcgga
    12541 gcttcggctg gtggcgcgag gcccggcacc ccgagctcgc ggtgctcgac gagctcggcc
    12601 tcggcgacgt cgcccaccgg ccggcgggcg agctggcgca tggccgccag cggctggtcg
    12661 agctcgccat ggtggtggcc gcccgaccgc gggcgatcct catggacgag cccttcgccg
    12721 gcctcgcccc ggacgaccgg ccggctctgg cccggcgcct cgccgcgctc aagtcgcgcc
    12781 atgccctgct gctcgtcgag cacgacatgg acgtggtctt ccagctcgcc gaccggatct
    12841 ccgtcctggt cgccggccgg ctcatcagga gcgacacgcc cgaggcgatc cgcgccgacc
    12901 ccgaggtgca gcgggcctat ctcagggacg aggattgatg ctggcggtcc acggcctcga
    12961 ggccgcctat ggcgcgaccc aggtgctctt cggcgtgtcg ctcgaggtcg gggaggggcg
    13021 gatcgtcggc ctgctcggcc gcaacggcat gggcaagacg acgctgatcc gctcgctcta
    13081 caacctgacc cgcaggctgg gcggcagcgt gcgtttcgag ggggaggacc tcgacccgct
    13141 gccgcccttc gccatcgccc ggcggggcat cgctgtggtg cccgaggggc gtcagatttt
    13201 tcccggcctc agcgtcgagg agaacctggt ggccaccgca cgcccaggcg aatggcgctt
    13261 gggacgggtg ctcgacctct tcccgcgcct cgccgaacgc cggcggaacc cgggcgggtc
    13321 gctctcgggc ggcgagcagc agatgctggc gatcggccgc gccctgctca ccaatccgag
    13381 gctgctggtg ctcgacgagg cgaccgaggg gctcgcaccg gcggtgcgcc gcgagatctg
    13441 gcagtgcatc cgcgcgctga aggcgaccgg catcgccatc ctcttggtcg acccgcatct
    13501 cggccggctg ctcgagctga ccgacacgaa cgtgatcatc gagaacgggc gcagcgtctg
    13561 gcagggcagc tcggccgagc tcgccgaccg gccggcgctg cagcgtcgct atctcgccgt
    13621 ctgagccggc ggtgtccgcc ggcccggatc agacggcttc gcggtgggct tcctgcagga
    13681 ggctgcgcat gtcgtcggcg gcccgctcga ggaagctcag cacccgggcg tcgccatagt
    13741 cgtagaagag gtcgttcggg tccttcgagg tctgcccgcg gacgcggccg agcgccagct
    13801 ccagcaccgc atggatcgaa tcgagcgagc gggccggggt ggcgagcagg tcgttctcga
    13861 gctcctcgat gcgctggtcg agctgctcca gctcgcgctc gatcgcgtcg agcccggcat
    13921 cgctctcgac gcggctcatc tcctgcatca tgccgcggat ctcggccgac gacatgccgg
    13981 cgagctgcag gggcgcgaag gcccactccg gcagggtgcg gcgcagctgg tcctggcgct
    14041 cgacgaggat gtcgcgccgc cgctggagat cgctgatgcg ccgcaccagc agggtcgaat
    14101 tgttgatgac gttcatcgtt ctcgatcctc cgctcgccga tcggccgtcg gaagtgcgcg
    14161 atgcacagca tatggatggc cgactaacgc gatccaatta gcgacgacgt cttgggcgtg
    14221 tcaaccgaat ttaaataaaa ccttagttga atatttatga aaaaagaaga taaaatttta
    14281 tccaaatttt attactgttt tccttgaatg atatgcaaga ccatggccag ccgacctgtg
    14341 cgtcgctgcg gcggtcggca cggtcgcgcg cggcggggcg ggctgctaac ctcgcgccta
    14401 tcgagagcga tggcggggaa gtgccgtggc gggcagcagg gacggccggc ggcggccggc
    14461 gggcgagggt ggcggtgagg gcgtggcgcg gtcgccatcg ctgctcgccc ggctgcgcac
    14521 ctatctcttc gccggcatcg tcgtcaccgc accggtcagc atcaccttct tcatcgtctg
    14581 gcagatcctc gacttcctcg accggacggc gagccggctg attcccgacc gctacgaccc
    14641 ggccagctac ctgccgttcg gcattcctgg gctcggcctc ctggtgatgc tgctgctcct
    14701 gatcttcatc ggctggttcg ccgccggctt cgtcggccgc tcgctgatgc gcgccggcga
    14761 gcgggtgctc gaccggatgc cggtcgtgcg ctcgatctac ggcacgctca agcagatctt
    14821 cgagacggtg ctggcgcaat cctcgcgctc gttccgcgag gtcgtgctgc tcgaatggcc
    14881 gaggcggggg ctctgggtga tcggtttcgt caccggcccc acctcgggcg agatcaagag
    14941 ccgcttcgac ggcgacttcg tcaacatctt cctgccgacg acgccgaacc cgacctcggg
    15001 attcctcgtc ttcgtcccgg cgcgcgacct gatccacctc gacatgagcg tcgaggaggg
    15061 catcaagctg gtcatctccg gcggcatcgt ggcgccggcg cgtgaccggg gcctgatggc
    15121 agggggcgcc gaggcccggc cgccggccgg cacttagcag acgtggccaa atcgcgggca
    15181 ggcttctagt ctccggcgcg ttcatcacga ggatcgccat gcccgacgtc gacgccacga
    15241 acctgctcgc ccggatcgcc gaggccctgg agcggcagag cccgccgccg gccctttcgc
    15301 tcgggctggg tgccgcccgc gcctatgtgt tcgatgcccc ggcccgggcg ctccggccgg
    15361 tgccgcgggt ggcgcgcatc gatttcgagc tgctctgcgg catcgaccac gtggcgcaga
    15421 tcctgctcga caacacccgc cgcttcgccc gcggcctgcc ggccaacaac gcgctcctct
    15481 ggggggcgcg cggcatgggc aagtcgagcc tggtcaaggc ggtgcacggg ctggtcgagc
    15541 gggaaggggc cgacctcatc ctgatcgaga tccaccgcga ggacatcgac tccctgccgc
    15601 atctgctgca cgacctcgcc cgccagccgg accgtttttt gctcttttgc gacgacctct
    15661 ccttcgatgc gggcgagagc agctacaagt cgctcaaggc cgtgctcgaa ggcgggctcg
    15721 aggggcggcc gcagaacgtc ctcttctatg ccacctccaa ccgccggcac ctgatgcccc
    15781 ggcagatgat cgacaacgaa cgcggcacgg cgatcaaccc cggcgaggcg gtcgaggaga
    15841 aggtctcgct ctccgaccgc ttcggcctct ggctcggctt ccatccctgc aaccaggaga
    15901 cctatctcgc catggtcgac cgctatgccg ggcatttccg cctggaggtc gagcccggcg
    15961 agctcaagcg ccaggccctc gaatggtcca ccacgcgcgg cagccgctcg ggccgcgtcg
    16021 ccttccagtt catccaggac ctggcaggcc ggctgggcaa gcggctcggc taggcttcta
    16081 ggacgtcagc ccctcgacca ggaatttctg cgtccgccgg gcgctctgca gccgccattc
    16141 gaggatccgg tgggtgagct cctggcgggt cgcctggtgc gccggcgcgt cccacagatt
    16201 ctcgacctcg tccgggtcgg cctcgaggtc gaagagctgg ccctcgcttg atcccaggta
    16261 gtgcacgagc ttccacttgc cggtgcggat catggtgacg aacgggcagt cctcctggat
    16321 catgtcgtcg gcgagctcgg catagaccgt gtcgcgcgcc gggtcggtgt cccggtcgaa
    16381 atacggcacg aggctccgcg cctccatcca ggccggcggg gcgacgccgg cgagctcgag
    16441 gatcgtgggg ccgaggtcga agagcgaggt caggtcgtcg atgcgccggc cgccatcctg
    16501 tcgcctgggg tcgtgcatga cgcaggggac gcggaccgag ctctcgaaca tgctccattt
    16561 ctgcgaatgg ccatggtcgt tcaggctgtc gccgtggtcc gaggtgaaga tgatgacggt
    16621 gtcgtcgagg accccccggg cctcgagcgc ggccttcagg tggccgagct gctcgtcgat
    16681 catggtgacg ttggcggcgt aatgggcgcg ctggcgctgc atctgctcag gcgtcgggtc
    16741 ggccagatgc acgacggcgt cgtggtcgtt cgcgatatgg ttctggcgca gcctgcgcat
    16801 ggccgtgggc tggctgtcga ggtcgtagtt gcggatcggc tgcgggaacg cgacgccgtc
    16861 atagagggcc agcgcctccg cggtcggatc gtagggcgga tgcgggcccg gaatgccgac
    16921 ctgcaggaag aagggctcct cgcccttgta ggattcgagc cagtggacag cactctgggc
    16981 gacgaagacg tcggggtgca gcttctccgg cagctcccag acgaaggcgc cgaggcggtc
    17041 cttgtagtcg gcgcggtggc agtaggtctg gcgtgacggc ttggtatggc cggcggccca
    17101 gagcgccttg tcccagttgt ccaggtagaa cggcagggcc ggatgcgccc ggtccttgtt
    17161 ctcggtgaca tggcgctcgt ggaagccgaa cggctcctcg aacgggtggg tgtgcatctt
    17221 gccgacattg acgcagcgat agcccgcctc agcgagcagg tggacccagg tgtagggcca
    17281 ccgctcgtcg ttgcgcagca cgccggagct gtgcgggtag agcccgctga agagcgaggc
    17341 gcgcgagggc gagcagacgg gcgacgtgca gtacatccgc gtgaaggcga cgccctcctc
    17401 gaccatgcgg tcgaggttcg gcgtgatcat gtggtcgtgg ccgagcgcct tgatcgtgtc
    17461 gtgccgctgc tgatcggtga tgatgaagac gat
//
LOCUS       DAOTFI010000100        17320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_3970_length_17320_cov_6.441066, whole genome shotgun sequence.
ACCESSION   DAOTFI010000100 DAOTFI010000000
VERSION     DAOTFI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17320
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_17320_cov_6.441066"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="P5558_11700"
     CDS             complement(<1..1003)
                     /locus_tag="P5558_11700"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25020.1"
                     /translation="MLHVDIPTLAEISRLAEARGEARISIYLPTTPLTQEAEADRIAL
                     KNLGDAAIEQLTEAGCDKRQIWPIEEQLEALHDDDEFWRLQARSLAVFLTPERHWTFR
                     LPNSLQATVQVSDRFHLKPLLRAVTFPNEALVLALSQNEVRLVEVFAELPPQEVKVPG
                     MPKDAPSAVGKATIGHRSPAGRLHGSEGEKVRLRQFARAVNGALRPLLSGRETPLVLA
                     STRPVDDIFRSVNTYPHLVDPQITKSPDKMTDLELAEAARPLLDQFYAAQVEELRQLY
                     AVRDSQGRATTDIAQAARAATFGAIDSLLVDIDDVVPGWVDETDGAVRFADGEDATSY
                     "
     gene            complement(1026..3776)
                     /locus_tag="P5558_11705"
     CDS             complement(1026..3776)
                     /locus_tag="P5558_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRY25021.1"
                     /translation="MTPTPEPGTEKAPGNGTPGPIDWAALAVAAVRTHLGTPDDGLDP
                     AEAARRLAEHGPNRLPVAPGRPAWRRLLAQFDNLLIYVLLAAAVLAGTFGHAVDAAVI
                     LAVVLANAIIGYVQEGRAEDALTSIRSMIDPEATVLRGGERFAVAAEAIVPGDLVLLE
                     AGDRVPADLRLVRTRNLAIDEAALTGESVPVEKSVEPTAAGAVLGERRSMAFSGTLVT
                     AGQGQGLVVATGSRSELGRISAMIGRVEELKTPLLRQMDQFARRVTFVVLAVSGLVFV
                     LALLRQMLALDEAFMVVVGLFVAAIPEGLPAVMTITLAIGVQRMAARHAIIRRLPAVE
                     TLGAVSVICSDKTGTLTRNEMTVRSVVTAEGMVEVAGSGYVPEGAFWQDGAAFDPGTS
                     PTLQALAESGLLCNDADLLRRDGGWQMTGDPMEGALLAFAIKAGLDPATHRRRLRRQD
                     AIPFDAAHRFMATLHVEGAGDPRRVLVKGAPERLLEMCGTVLTATGTEPLDHAAWQQR
                     IAELAERGERVLAFACKPRHEGDGIDHADLDDGLVLIGLAGFIDPPRDEAIAAVESCH
                     AAGIRVVMITGDHALTARSIARRLGLAENPVVLTGRDLDAMDDAALGRAALETHVFAR
                     TTPEHKLRLVQALQAAGLTVAMTGDGVNDAPALKRADVGVAMGRKGTAAARDASEMVL
                     ADDNFASIVDAVREGRTVYDNLTKVIAWTLPTNGGEALTIIVAVLAGLALPITPVQIL
                     WVNMVTALALGLTLAFEPTEPGTMARPPRPAGEPLLERDVVWRVFSVSVLFVIGAYGV
                     FYWSISRGLPIEAARTLVVNAIVAMEIFYLFSVRYVHGASLSWRGMLGTRAVLIGVAT
                     VTVLQVAFTYAPWLQLAFDTRALAVADGLVAVAVGVALFVVVEVEKIVRSRACGAMRH
                     GN"
     gene            3924..4640
                     /gene="nanR"
                     /locus_tag="P5558_11710"
     CDS             3924..4640
                     /gene="nanR"
                     /locus_tag="P5558_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HRY25022.1"
                     /translation="MPPVAPIQRRKLFQEVLERLLERLRAGEFQPGDQLPSERELMQF
                     YGVGRPAVREALQALERDGIVTITHGERARVALPSAERVIEQIAESTRHLLQVEPGNL
                     DHLKDARLFLETGLASRAAERATPEAVARLEERFAAHEAARQQADAFLPRDMEFHREI
                     AAMVGNPIFPALIEAMFDWLGSYYVQLVRLPGAEDLTIAEHRRILDAIAAGDGDAAAA
                     AMHDHLTRANALYRQFEKSG"
     gene            4677..6599
                     /locus_tag="P5558_11715"
     CDS             4677..6599
                     /locus_tag="P5558_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25023.1"
                     /translation="MAANDDPILYGTTQPPTPMQRLEAGPLSIDLEGAAVRYVRYARL
                     EAIRGIDYLVRDASWRTPPAALTTLRQEQDESGFTVELEGVVRQDEIDYHYRQTITGA
                     ADGRLDVVVEAEAKNGFLTNRTGFVVLHPILGVAGQPVTVTHKDGRASETTFPELISP
                     GQPIFDIRALRHEMAPGLFVTCRMEAALPHDPETIFEMEDQRNWTDASYKTYVGSLLD
                     PWPYRIEPGQRLRQRITVTFEGSAPARPSDTTNGVGLRLGDTVAGRVPAIGLGVMPEH
                     RAAAGSDSNPAARLRPAFITAYAEVGSDDFAQSLADYAGLAQRLGAAVQLELVLPAGR
                     RPAEALDEAAAACKAAGLAPERVLPCPAPYLKSIQPVGPWPDVADLGHIYNQARRAFP
                     RSAILGGMCSYFTELNRKRPPAATIDGISCTTTPIVHAADDRSVMETLEALPAVLRSM
                     AAMAPGKALHIGPSAIAMRHNPYGTATAGNPKAERIAMAEDDPRQRGLLAAAWSVGYA
                     TAMAEGGVATLSLNHLAGPSGLASESGETWPVFHVMAALCAASGAERVPVETGGEGVA
                     GLAWLEDGKTRLLVANLRPEPTALRLDRPVTGHLLDAAAAAGPARAPDWPPTAAPAWH
                     GTETTLPPLAVLFAGT"
     gene            6684..7697
                     /locus_tag="P5558_11720"
     CDS             6684..7697
                     /locus_tag="P5558_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY25024.1"
                     /translation="MRKIEPMVSRRSFTVGALLAAGTAGLPIMVKAQEGKTVALLFDS
                     LVSPFWVSGIEIMRAEAAERGWETLEAISDFDDNRQFEQVRAMIEREVDGILIIQTDA
                     NAVIPAIRAANEAGIPMVHFNRPPAESDAYSVAIQADNRKIMQDTVQFLVDEAKAMGG
                     TYKACILIGDLGDVNAIQRRDGFFDIVDQHPDLIEVVARVSTEWNADKAFAGLTNALQ
                     ANPDINFLVTSSDFLHPQIEQALKIAGKWHPRGEEGHVLFGGFDGDEGAYQRLIDGYL
                     DACGVQNLFLEVDMAFGAFEDLWAGKQVDKLLLDPGFVITAANLEEVRDQMWGYIVWQ
                     QQQ"
     gene            7812..8858
                     /locus_tag="P5558_11725"
     CDS             7812..8858
                     /locus_tag="P5558_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25025.1"
                     /translation="MLRTAGASGNAATRTAGGVRTRGWLERLILSEYLVFYLTTAYFL
                     AVWPIVPEIATASSLSNMLTDMLPLLVVAIGQTFVLIVAGIDLSVTSIIAFASVVGAS
                     VMTADGGYLGGSPLAVPAGILTFLLIGGLIGWFNGFCCTRLGMPPIIVTLTGLMFLSG
                     TAIWYTTGHSETSSIAGLPKAFVLIGQGSAYGVPNALWVALAVGISAHLLLGHTVFGR
                     WLYAIGINRKAAEISGVPVGTAILWTFVISGVCAAIASILFTGRLETGTPVLGQRILL
                     DIIAAVVIGGTSLAGGKGKIVWTVGGVLFLVVIDTSLKLLGMSLFVVFAVKGGVILFA
                     AIIDAMRQRIIMRG"
     gene            8861..10384
                     /locus_tag="P5558_11730"
     CDS             8861..10384
                     /locus_tag="P5558_11730"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY25026.1"
                     /translation="MAAEILRLDGLSKSFFGVEVLHAVSFALHRGEILGLVGENGSGK
                     STTMNILGGIHQADAGSITLDGRPYRPTGPRDAQEAGLAFIHQELNLFENLSIEENIF
                     ISGFPRRLGRLPLIDRRRIGQRTRELLDMVDVRYPPATPVGKLSQGERQLVEIAKALS
                     QDARIILLDEPTTSLTRRETEKLFTIMERLRAQGIALIYISHILGDVLRVCDTIQVLR
                     DGHLVGGGPRDEMPIDTLISLMVGRKIENLFPPRDTEPTTVPALEVEGITQSGVVRDV
                     SFTLHEGEVLGVAGLMGAGRSELARILFGLDPFEAGRIAVGDTTLTEVSPEACMRHGM
                     AFLTEDRRGEGLMMEATIADNTALPSLRAYARSLTGWIARSRLEDDVAEVSRRVQINT
                     TDYRGMLVKNLSGGNQQKVVIGKWLLRQPKVFILDEPTRGIDVGAKFEVYKIVNQLVA
                     EGTAVLFISSEMEELIGMCDRILVMSHGEITGIHERHEFDSERILETAMLKNLADVA"
     gene            10384..11370
                     /locus_tag="P5558_11735"
     CDS             10384..11370
                     /locus_tag="P5558_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25027.1"
                     /translation="MSGTAGRRLALVVLQNAPLIAFVVVLVIFASLSDRFLTLGNFTN
                     IVTQSSHIAIMAIGITFVLLTAGIDLSVGAVMYVGVAILALYLGDLPVVVSFPLVALI
                     GLALGAVNGFFVVQMRGLHPFIITLAMLFILRGAALWMTDTRMVFVAEPIMSLGRTSY
                     LGVPWAIWMFAAVFALAWIVLRETPFGRQVYAVGQDPVAAGKAGINVPLVLFAVYCIC
                     GLCAALGGLVSIAQVAAASSTFGFQKEFPVIAAAVLGGTSLFGGRGGVIGTVFGAILI
                     QTVENGLVMTNANPYIYPLVTATIIFLAVLIDSQRTRILEAMGRRKIRVEEA"
     gene            11498..11881
                     /locus_tag="P5558_11740"
     CDS             11498..11881
                     /locus_tag="P5558_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HRY25028.1"
                     /translation="MLLATLTPFPVVCFTLALLTDLAYWRTSYLMWQHFSAWLLLVGI
                     VVGGCALLVSIIATLTHRPGHRWSHIGLGVLVLALAFLNNLVHARDGWTAVVPSGLVL
                     SALTVAALIASGLMRRASASSRWSS"
     gene            11878..13329
                     /locus_tag="P5558_11745"
     CDS             11878..13329
                     /locus_tag="P5558_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HRY25029.1"
                     /translation="MSRTTYAAVGAIGLLALAACDDGPDDFDVSRQIGPDPVLPEPRQ
                     PLLASIKLAEVVGWQEGQTPVVPESLTVSAYATDLANPRTVHTLPNGDVLVVQSRGPS
                     GKPLPRPKDYIRGWIMSLAKGGGGEQPESNLVTLLRDTDRDGMVDERSDLLTGLHSPF
                     GVAWHDGTLYVAEADEIVAYPYELGATEVGGEPQTITPLPGGPINHHWTKDLALGPDA
                     RHLYVSVGSNSNAAERGMEAEKGRAAIWEVDRATGASRVYASGLRNPNGLTFHPETGT
                     LWTVVNERDELGPNLVPDYMTSVQDGGFYGWPWSYYGDHVDVRVHPPRPEMVEQAIAP
                     DYALSSHVAPLGMAFTMGSAMPEAFANGAFVGEHGSWNRTFFNGYKVVYIPFENGMPA
                     EDVVTGFLDGDQARGRPVGVAIDGTGALLIADDAGNTVWRVAAADGSVTPEPIGSDRV
                     AAERAAPPDAVGPAPSGEAATPPPTEIAPAVQE"
     gene            complement(13331..13564)
                     /locus_tag="P5558_11750"
     CDS             complement(13331..13564)
                     /locus_tag="P5558_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25030.1"
                     /translation="MNLALTATQEELVRLLVLRLREGGLEFERRVREFVQEAPVPRYM
                     HVDELDRRFGALERRILALEQALDGRIDADRVA"
     gene            13776..14204
                     /locus_tag="P5558_11755"
     CDS             13776..14204
                     /locus_tag="P5558_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25031.1"
                     /translation="MLTSLYVVIMATLYIGHTWYLILLKRHARIRQVDWPMRCYDRAS
                     HGRNFVAQYTPLALILLAMVEIGGAPALAMHGLGIMLVAAALLHCHSFGFPARNSSGV
                     AAMLLVTSMYAITMTLALAQYAGVDDLPNAPLMALLAHVR"
     gene            14201..14590
                     /locus_tag="P5558_11760"
     CDS             14201..14590
                     /locus_tag="P5558_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25032.1"
                     /translation="MTPALDGDGALAYILRRLRQQGPATHGQHGYHLTVVDLAARYVA
                     EVMGKRHPDPIENEDTYAASRPLYDAAWELARRGILRPSVHSSFMQFDAYQSAGGGYT
                     LTAIGAEWLSELDDRQLSLRAAWGPFT"
     gene            14685..15692
                     /locus_tag="P5558_11765"
     CDS             14685..15692
                     /locus_tag="P5558_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY25033.1"
                     /translation="MLKGGIIGCGFFAANHLAAWQLIDGAAIVAVCDQDPARARAAGA
                     AGDGARVFDDAATMLASEALDFVDIITTAPSHRPLVELAARHRVPVICQKPFAPTLDD
                     ARAMVDACAQAGVPLMVHENFRWQSPLMAVKNIVSSGEIGEPAYARISFRHAYDIYTN
                     QPYLKTEPQLAIMDLGIHLLDLARYFLGEATRLHCRTQRVNPAVAGEDCVTILLDHAG
                     GAVSLVDFSFFTKLEPDPFPQTLVRIEGTKGTVELLAGYHLRVSGPGWSREEDVEPAV
                     PAFGAKPWHNIQESVVNIQRHWIECLQNDREPATSGADNLKTIDLVFRAYESAATGRA
                     S"
     gene            15689..16705
                     /locus_tag="P5558_11770"
     CDS             15689..16705
                     /locus_tag="P5558_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY25034.1"
                     /translation="MSALRGAVIGCGFFAQNHLKAWGDIAGAELVAVCDLDPAKAAAA
                     AALTGAEAFTDVDAMLAAARPDFVDIATTMETHEPLVAKAAAAGADIICQKPFAPDIA
                     GVRRILQTAEAAGIRLMVHENFRFQSPLIELRRAMPAIGEPFFAHIAWRTGYDVVAGQ
                     PYLAKVERFILLDLGIHVLDVARFLLGDVADLYARTQRTMPGARGEAQATMTLRHRSG
                     AVCAVTCTYTNRIDPDPFPQTLAEIEGRDGTLRLERDYRLELHRPGSLEVRDIRPGVP
                     SWADPQWALAQGSVLNTQQHFVDCLKSGAEFQTSGRDNLKTFALVEAAYASAASGQPV
                     VPEA"
     gene            16702..>17320
                     /locus_tag="P5558_11775"
     CDS             16702..>17320
                     /locus_tag="P5558_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HRY25035.1"
                     /translation="MTERIRASYLIETADDPRRAVEVMAGEQSSGTFVALPGETPELK
                     ERYGARIEKLELLEEAATPSLPGAKAGPVYRRCAAELSWPLENLGASLPNLVATVAGN
                     LFELHQFSGLRLLDIDLPPAFAGRYPGPQFGIEGTRRLAGVASGPLIGTIIKPSVGLS
                     PEATAEMVDALCRGGIDFIKDDELQADGPHCPFEARARAVMQVIDR"
BASE COUNT         2645 a         6367 c         5663 g         2645 t
ORIGIN      
        1 catacgaggt ggcatcctcg ccgtcggcga agcgcaccgc gccgtcggtc tcgtcgaccc
       61 agccgggcac cacgtcgtcg atgtcgacca ggagcgaatc gatggcgccg aaggtggcgg
      121 cgcgcgccgc ctgggcgatg tcggtggtcg cccggccctg gctgtcgcgg acggcgtaga
      181 gctgtcgcag ctcctcgacc tgggccgcgt agaactggtc cagaagcggc cgcgccgcct
      241 ccgccagctc gagatccgtc atcttgtccg ggctcttggt gatctgcggg tcgacgagat
      301 gcgggtaggt gttgaccgag cggaagatgt cgtcgaccgg tcgcgtggag gccaggacga
      361 gcggcgtctc gcggcccgac aggagcgggc gcagggcgcc gttcaccgcc cgcgcgaatt
      421 gccgcagacg caccttctcg ccctccgagc cgtgcagccg gccggccggc gagcgatggc
      481 cgatcgtcgc cttgccgacg gcgctcggcg cgtccttcgg catgccgggc accttgacct
      541 cctgcggcgg cagctcggcg aacacctcga ccaaccgcac ctcgttctgc gagagcgcca
      601 gcacgagcgc ctcgttgggg aaggtcacgg cccgcagcag cggcttgaga tggaagcggt
      661 cggaaacctg cacggtggcc tgcaggctgt tcggcagccg gaacgtccag tgccgctcgg
      721 gcgtcaggaa caccgccagg ctgcgggcct gcagccgcca gaactcgtcg tcgtcgtgca
      781 gcgcctcgag ctgctcctcg atcggccata tctgccgctt gtcgcaaccg gcttccgtga
      841 gctgctcgat ggccgcatcg cccaggttct tcagggcgat gcggtcggcc tcggcctcct
      901 gcgtcagggg cgtggtcggc aggtagatcg agatgcgggc ctcgccccgc gcctcggcca
      961 gcctgctgat ctcggcaagg gtcggtatgt cgacatgcaa catcggtcgt gcacttcctc
     1021 gggtttcaat tgccgtgtcg catagccccg caggcccggg aacgcacgat cttttcgacc
     1081 tcgacgacga caaagagcgc cacgcccacc gccacggcca cgagtccgtc cgctaccgcg
     1141 agcgcgcgcg tgtcgaacgc cagctgcagc catggtgcat aggtgaacgc cacctgcagc
     1201 acggtcacgg tggcgacacc gatgagcacc gcccgggtgc cgagcatgcc ccgccagctc
     1261 agcgaggcgc cgtgcacgta gcggacgctg aagagataga agatctccat cgcgacgatc
     1321 gcgttgacga ccagtgtccg cgcggcctcg atcggcaggc cccgcgagat gctccagtag
     1381 aagacgccgt aggcgccgat cacgaagagc accgagaccg aaaagacgcg ccagaccacg
     1441 tcgcgctcga gcaggggctc gcccgccggc cgcggcggcc gcgccatggt gccgggctcg
     1501 gtcggctcga acgccagggt gagcccgagg gcgagggccg tcaccatgtt gacccagagg
     1561 atctgcaccg gcgtgatcgg caaggcgagg ccggcgagca ccgcgacgat gatcgtcagc
     1621 gcctcgccgc cattggtggg cagggtccag gcgatgacct tggtcaggtt gtcgtagacc
     1681 gtccggccct cccgcacggc atccacgatg gaggcgaaat tgtcgtcggc gagcaccatc
     1741 tcgctggcgt cccgggccgc ggccgtcccc ttgcggccca tcgccacgcc cacgtcggcg
     1801 cgcttgaggg ccggcgcgtc gttgaccccg tcccccgtca tggcgacggt cagcccggcc
     1861 gcctggagcg cctggacgag acggagcttg tgctccggcg tggtgcgggc gaagacatgc
     1921 gtctcgagcg ccgcccgacc gagcgccgcg tcgtccatgg cgtcgaggtc gcgacccgtc
     1981 agcacgacgg gattctcggc caggccgagg cggcgggcga tcgagcgcgc ggtgagcgcg
     2041 tggtcgccgg tgatcatgac gactcggatg ccggccgcat gacagctctc gaccgctgct
     2101 atcgcctcgt ccctgggcgg gtcgatgaag ccggcaaggc cgatcagcac gagcccgtcg
     2161 tcgaggtcgg cgtggtcgat gccatcgccc tcgtgccgcg gcttgcaggc gaaggccagc
     2221 acccgctcgc cacgctcggc cagctccgcg atccgctgct gccaggccgc gtggtcgagc
     2281 ggctccgtac cggtggcggt gagcaccgtg ccgcacatct ccagcagccg ttccggcgcc
     2341 cccttgacca gcacccgcct gggatcgccc gccccctcca catgcagcgt cgccatgaag
     2401 cgatgcgcgg catcgaaggg aatggcgtcc tggcggcgca gccggcggcg atgcgtcgcc
     2461 gggtcgaggc cggccttgat ggcgaacgcc agcagtgcgc cctccatcgg atcgccggtc
     2521 atctgccagc cgccgtcgcg ccggagaaga tccgcgtcgt tgcagagcag cccgctctcg
     2581 gccagcgcct gaagtgtcgg gctggtcccc ggatcgaagg cggctccgtc ctgccagaag
     2641 gcgccctccg gcacgtagcc gctgccggcg acctcgacca tgccctcggc cgtcacgacc
     2701 gagcgcacgg tcatctcgtt gcgggtcaac gtgccggtct tgtccgagca gatcaccgag
     2761 accgcaccca gggtctcgac cgccggcagg cgcctgatga tggcgtggcg ggccgccatg
     2821 cgctgcacgc cgatggcgag cgtgatggtc atcaccgccg gcaggccctc cggaatggcg
     2881 gcgacgaaga ggccgaccac caccatgaag gcttcgtcca acgcgagcat ctggcgaagc
     2941 agcgccagga cgaaaaccag gccggagacg gccagcacga cgaaggtcac gcgccgggcg
     3001 aactggtcca tctgccgcag gagcggcgtc ttcagctcct cgacccggcc gatcatcgcg
     3061 ctgatccggc cgagctcgct ccggctgccc gtcgccacca cgaggccctg cccctgtccc
     3121 gccgtgacca gcgtgccgga gaaggccatc gagcgacgct cgcccagcac ggccccggcg
     3181 gcggtcggct cgaccgactt ctcgaccggc accgactcgc ccgtgagagc ggcctcgtcg
     3241 atcgccaggt tgcgggtgcg gacgaggcgc aggtcggccg gcacccggtc tcccgcctcc
     3301 agcagcacga gatcgcccgg cacgatcgcc tcggccgcga cggcgaaccg ctcgccgccc
     3361 cgcaggaccg tggcctccgg gtcgatcatc gagcggatcg aggtcagggc gtcctcggcc
     3421 ctaccctcct gaacatagcc gatgatcgca ttggcgagga cgacggcgag gatgacggcg
     3481 gcatcgaccg catgaccgaa ggtgccggcg agcactgcag cggccaggag gacatagatc
     3541 aggagattgt cgaactgggc cagcaggcgc cgccaggctg gccgcccggg cgcaaccggc
     3601 aggcggttgg ggccgtgctc ggccagccgg cgggcggcct ccgccgggtc cagcccgtcg
     3661 tccggcgtcc ccaggtgcgt gcgcaccgcg gcgaccgcca gggccgccca atcgatcggg
     3721 cctggcgtgc cattgccggg ggccttctcg gtgccgggtt cgggcgtcgg ggtcactggc
     3781 gttcagctcc ggaaatccgc tgcactgcac acgcgcatat atagggacgg tgcccgaccc
     3841 cgaattgggg catttcgcca tcgtcgaggt ccttggcaag cgaccgtcgt tgcgccaagt
     3901 tgtgatgagg aggacatcat accatgcccc ccgtcgcgcc catccagcgt cgcaagctct
     3961 tccaggaagt gctggagcgt ctgctcgaac gcctgcgcgc cggcgagttc cagccgggcg
     4021 accagttgcc gagcgaacgc gagctcatgc agttctacgg cgtcggccga ccggccgtgc
     4081 gcgaggcgct gcaggcgctg gagcgggacg gcatcgtcac catcacccat ggcgagcggg
     4141 cgcgggtcgc gctgccctcg gccgagcggg tgatcgagca gatcgccgag agcacccgcc
     4201 atctgctgca ggtcgagccg ggcaatctcg accacctgaa ggacgcgcgg ctcttcctcg
     4261 agaccggcct cgccagccgc gccgccgagc gcgccacgcc cgaggccgtc gcacggctcg
     4321 aggagcgctt cgccgcccac gaggcggcga ggcagcaggc cgatgcgttc ctgccccgcg
     4381 atatggagtt ccaccgcgag atcgcggcca tggtcggcaa tccgatcttc ccggccctca
     4441 tcgaggccat gttcgactgg ctcggcagct actatgtcca actggtgcgg ctgccgggtg
     4501 cggaggacct gaccatcgcc gagcaccggc ggattctcga tgccatcgcc gccggcgacg
     4561 gcgatgccgc ggcggcggcc atgcacgatc atctcacccg cgcgaatgcg ctctatcgcc
     4621 agttcgagaa gagcggctga gcgcgccgcg accacaaagg aacaatggga ggcagcatgg
     4681 ctgcgaacga cgatccgatc ctctacggca cgacgcagcc gccgaccccg atgcagcggc
     4741 tcgaggccgg gcctctgtcg atcgacctcg agggtgccgc ggtccgctat gtgcgctacg
     4801 cccgcctcga ggccatccgg ggcatcgact acctcgtgcg cgacgcgagc tggcggaccc
     4861 cgcctgccgc gctcaccacg ctccgccagg agcaggacga gagcggcttc acggtcgagc
     4921 tggagggcgt cgtccgccag gacgagatcg actaccacta ccgccagacc atcaccggtg
     4981 ccgccgacgg gcgactcgac gtcgtggtcg aggccgaggc gaagaacggc ttcctcacca
     5041 accgcacggg cttcgtcgtc ctccacccga tcctcggcgt cgccggccag ccggtcaccg
     5101 tgacccacaa ggacggcagg gccagcgaga cgaccttccc ggagctcatc agcccgggcc
     5161 agccgatctt cgacatccgc gccctgcgcc acgagatggc acccggcctc ttcgtcacct
     5221 gccggatgga agcggcgctg ccgcacgacc cggaaaccat cttcgagatg gaggaccagc
     5281 gcaactggac cgacgcctcc tacaagacct atgtcggctc gctgctcgat ccctggccct
     5341 accggatcga gccgggccag cggctgcgcc agcgcatcac cgtcaccttc gagggcagcg
     5401 cgcctgcccg gccctccgac acgacgaacg gcgtcgggct gcgcctcggc gacaccgttg
     5461 ccggacgagt gccggcgatc ggcctcgggg ttatgccgga gcatcgcgcg gcagccgggt
     5521 ccgacagcaa tcccgccgcc cggctccgcc ccgccttcat caccgcctac gcggaggtcg
     5581 gcagcgacga cttcgcccag agcctcgccg actatgcggg cctcgcgcag cgactgggcg
     5641 ctgccgtgca gctcgagctg gtcctgcccg ccggccggcg cccggccgag gccctggacg
     5701 aggcggccgc cgcctgcaag gccgccggcc tcgcgcccga gcgcgtcctg ccctgccccg
     5761 ccccctacct gaagagcatc cagccggtcg gcccctggcc ggacgtggcc gacctcggcc
     5821 acatctacaa ccaggcgcgc agggccttcc cgaggagcgc catcctcggc ggcatgtgca
     5881 gctacttcac cgagctgaac cgcaagcgcc cgccggccgc gaccatcgac ggcatctcct
     5941 gcaccaccac gccgatcgtc catgccgccg acgaccgctc ggtgatggag acgctcgagg
     6001 ccctgccggc cgtgctccgg agcatggccg cgatggcgcc gggcaaagcg ctgcatatcg
     6061 ggccgtcggc catcgccatg cgccacaacc cctacggcac ggccaccgcc ggcaacccga
     6121 aggccgagcg gatcgccatg gccgaggacg acccgcggca gcggggtctc ctcgccgccg
     6181 cctggagcgt cggctacgcc accgccatgg ccgaaggcgg cgtcgcgacc ctgtcgctca
     6241 accacctggc cgggccttcc gggctggctt ccgagagcgg cgagacctgg cccgtcttcc
     6301 acgtcatggc cgcgctgtgc gccgccagcg gcgccgagcg cgtgccggtc gagacagggg
     6361 gcgaaggcgt ggcgggactg gcatggctgg aggacggaaa gacccgcctg ctggtcgcca
     6421 acctccgtcc ggagccgacg gcgctccgcc tcgaccgacc ggtgacgggg catctgctcg
     6481 atgcggcggc cgccgccggg ccggcgcggg cgcctgactg gccgcccacc gcagcaccgg
     6541 cctggcatgg gaccgaaacg accctgccgc ctcttgccgt gctcttcgcc ggcacttgag
     6601 ccgaggggcc gagtggtata ctcagcagcc aaccaaaggc gccgccaccg ctgcgccttt
     6661 cagtgccaac cagggaggtg atcatgcgta aaatcgaacc gatggtctcg cgtcgttcgt
     6721 tcactgtcgg tgccctgctc gccgcaggca cggccggcct gccgatcatg gtcaaggcgc
     6781 aggagggcaa gaccgtggcc ctcctcttcg acagcctcgt ctcgcccttc tgggtgtccg
     6841 gcatcgagat catgcgcgcc gaagcggcgg agcgcggctg ggagaccctc gaggccatct
     6901 cggacttcga cgacaaccgc cagttcgagc aggttcgcgc catgatcgag cgcgaggtcg
     6961 acggcatcct gatcatccag accgacgcca acgcggtcat cccggcgatc cgggcggcca
     7021 acgaggccgg catcccgatg gtccacttca accgcccgcc ggccgagagc gacgcctatt
     7081 cggtggccat ccaggccgac aaccgcaaga tcatgcagga caccgtccag ttcctggtcg
     7141 acgaggccaa ggccatgggc ggcacctaca aggcctgcat cctgatcggc gacctcggcg
     7201 acgtgaacgc catccagcgc cgcgacggct tcttcgacat cgtcgaccag catcccgacc
     7261 tgatcgaggt cgtggcccgc gtctcgaccg aatggaacgc cgacaaggcg ttcgccggcc
     7321 tgaccaacgc gctgcaggcc aaccccgaca tcaacttcct cgtcacctcc tcggacttcc
     7381 tccacccgca gatcgagcag gcgctcaaga tcgccggcaa gtggcatccg cgcggcgaag
     7441 agggccacgt cctgttcggc gggttcgacg gcgacgaggg cgcctaccag cggctgatcg
     7501 acggctatct cgacgcctgc ggcgtgcaga acctcttcct cgaggtcgac atggcgttcg
     7561 gcgccttcga ggacctctgg gccggcaagc aggtcgacaa gctcctgctc gacccgggct
     7621 tcgtcatcac cgcggccaac ctcgaggagg tccgcgacca gatgtggggc tacatcgtct
     7681 ggcagcagca gcaatagcct cgtcatgatc caccggcccg cgccgacaag cgcgggccgg
     7741 ccctttgccg caggcgagaa ggacgtgggt gagttggcgg tgacgatgag ccagccgagc
     7801 tgggatctgc cgtgctgagg acggccggtg cttccggcaa tgccgcgacc aggaccgctg
     7861 gcggtgtccg gacgcggggt tggctggagc ggctgatcct ctccgagtat ctcgtcttct
     7921 atctgaccac cgcctacttc ctggcggtct ggccgatcgt gcccgagatc gcgacggcga
     7981 gctcgctctc aaacatgctc accgacatgc tgcccctcct ggtggtggcg atcggccaga
     8041 cgttcgtcct gatcgtcgcc ggcatcgacc tctcggtcac cagcatcatc gccttcgcca
     8101 gcgtcgtcgg tgcctcggtg atgaccgccg acggcggcta tctcggcggc tcgccgctcg
     8161 ccgtgccggc cggcatcctg accttcctgc tcatcggcgg gctgatcggc tggttcaacg
     8221 gcttctgctg cacccggctc ggcatgccgc ccatcatcgt gacactcacc ggcctgatgt
     8281 tcctcagcgg gacggccatc tggtacacga ccgggcacag cgagacgagc agcatcgccg
     8341 gcctgcccaa ggccttcgtc ctgatcggcc agggcagcgc ctatggcgtg ccgaacgcgc
     8401 tctgggtggc gctcgccgtg ggcatctccg cccatctcct gctcggccac acggtcttcg
     8461 gccgctggct ctacgccatc ggcatcaacc gcaaggcggc ggaaatctcc ggcgtgccgg
     8521 tcggcacggc catcctctgg accttcgtga tctcgggcgt ctgcgccgcc atcgcctcga
     8581 tcctcttcac cggccggctc gagaccggca cgccggtgct cggccagcgc atcctgctcg
     8641 atatcatcgc cgccgtggtg atcggcggca ccagcctcgc cggcggcaag ggcaagatcg
     8701 tctggaccgt gggcggcgtc ctcttcctcg tggtcatcga caccagcctc aagctgctcg
     8761 gcatgtcgct cttcgtcgtc ttcgcggtca agggcggggt catcctcttc gccgcgatca
     8821 tcgacgccat gcgccagcgc atcatcatgc ggggctagag atggcagccg agatcctgcg
     8881 cctcgacggc ctctccaaga gcttcttcgg cgtcgaggtg ctgcacgcgg tgagcttcgc
     8941 gctgcaccgg ggcgagatcc tcggcctcgt cggcgagaac ggctcgggca agtcgacgac
     9001 gatgaacatc ctgggcggca tccaccaggc ggatgccggc agcatcaccc tcgacggccg
     9061 gccctaccgg ccgaccgggc cgcgcgacgc ccaggaggcc ggcctcgcct tcatccatca
     9121 ggagctcaac ctcttcgaga acctctcgat cgaggagaac atcttcatca gcggcttccc
     9181 ccggcgcctc ggccggctgc cgctgatcga tcggcgccgc atcggccagc gcacgcgcga
     9241 gctgctcgac atggtcgacg tccgctaccc gcccgccacc ccggtcggca agctcagcca
     9301 gggcgagcgg cagctcgtcg agatcgccaa ggcgctgagc caggacgccc gcatcatcct
     9361 gctcgacgag ccgaccacct cgctcaccag acgcgagacc gagaagctct tcaccatcat
     9421 ggagcggctg cgcgcacagg gcatagcact gatctacatc agccacattc tgggcgacgt
     9481 gctgcgcgtc tgcgacacca tccaggtgct gcgcgacggc catctggtcg gcggcgggcc
     9541 gcgcgacgag atgcccatcg acacgctgat ctcgctcatg gtcggtcgca agatcgagaa
     9601 cctgttcccg ccccgcgaca ctgagcccac caccgttccc gcactcgagg tcgagggcat
     9661 cacccagtcc ggcgtggtcc gggatgtctc cttcaccctg cacgaaggcg aggtcctggg
     9721 cgtcgccggg ctgatgggtg ccggccgctc cgagctcgcc cgcatcctct tcggcctcga
     9781 cccgttcgag gccggccgca tcgccgtcgg cgacacgacc ctgaccgagg tctcgcccga
     9841 agcctgcatg cgccacggca tggcgttcct gaccgaggac cggcgtggcg agggcctgat
     9901 gatggaggcc acgatcgccg acaacaccgc cctgccctcg ctccgcgcct atgcgcgctc
     9961 gctcaccggc tggatcgcgc gcagccggct cgaggacgat gtcgccgagg tcagccggcg
    10021 ggtccagatc aacaccaccg actaccgcgg catgctggtc aagaacctgt cgggcggcaa
    10081 ccagcagaag gtcgtgatcg gcaagtggct gctccgccag ccgaaggtct tcatcctcga
    10141 cgagccgacc cgcggcatcg acgtcggtgc gaagttcgag gtctacaaga tcgtcaacca
    10201 gctggtcgcc gagggcaccg ccgtgctctt catctcgtcc gagatggagg agctgatcgg
    10261 catgtgcgac cgcatcctcg tgatgagcca cggcgagatc accggcatcc acgagcgcca
    10321 cgagttcgac agcgagcgca tcctcgagac ggccatgctc aagaatctgg cggacgtggc
    10381 ctgatgtcgg gaaccgccgg ccgccgcctc gccctcgtgg tcctgcagaa cgccccgctg
    10441 atcgcgttcg tcgtggtgct ggtcatcttc gccagcctct ccgaccgctt cctgacgctc
    10501 ggcaacttca ccaacatcgt cacccagtcg tcgcatatcg cgatcatggc gatcggcatc
    10561 accttcgtgc tgctcaccgc cggcatcgac ctctcggtgg gtgcggtgat gtatgtcggc
    10621 gtcgccatcc tcgccctcta cctgggcgac ctgccggtcg tcgtctcctt cccgctggtc
    10681 gccctgatcg gtctcgccct cggtgccgtc aacggcttct tcgtcgtcca gatgcggggc
    10741 ctgcacccct tcatcatcac gctggcgatg ctgttcatcc tgcgcggtgc cgccctctgg
    10801 atgaccgaca cccgcatggt gttcgtcgcc gagccgatca tgagcctcgg ccggaccagc
    10861 tatctgggcg tgccctgggc gatctggatg ttcgctgcgg tcttcgccct ggcctggatc
    10921 gtgctgcgcg agacgccctt cggccgccag gtctatgccg tgggccagga cccggtcgcc
    10981 gccggcaagg ccggcatcaa cgtgccgctg gtgctgttcg cggtctactg catctgcggc
    11041 ctctgcgcgg cgctcggcgg cctcgtctcg atcgcccagg tggcggccgc ctcctcgacc
    11101 ttcggcttcc agaaggagtt cccggtgatc gccgccgccg tgctcggcgg caccagcctc
    11161 ttcggcgggc gcggcggggt gatcggcacc gtcttcggcg ccatcctcat ccagaccgtc
    11221 gagaacggcc tcgtgatgac caacgccaac ccctacatct acccgctggt gacggcgacc
    11281 atcatcttcc tcgccgtgct catcgacagc cagcggacgc gcatcctcga agcgatgggc
    11341 cggcgcaaga tccgggtcga ggaggcctga cgccgggcgc agtcttcagg aacccctgcc
    11401 gtccccggcc gttcgggagg cggcaccact ggagactgcg atggctcatg cacggcccgt
    11461 tgacgggtat cgccgggagc gtccacgcta cgcggacatg cttctggcaa ccctgacacc
    11521 cttccctgtc gtctgcttca cgctcgcgct gctgaccgac ctcgcctact ggcgaacctc
    11581 ctacctcatg tggcagcact tctcggcctg gctgctgctg gtcggcatcg tggtgggcgg
    11641 ctgtgcgctg ctcgtgagca tcatcgcgac gctgacgcac cggccgggcc atcggtggtc
    11701 tcacatcggc ctcggcgtcc tcgtgctggc gctcgccttt ctgaacaacc tggtccacgc
    11761 ccgggacgga tggaccgctg tcgtgccgag tggcctcgtg ctctccgcac tgaccgtcgc
    11821 ggcactcatc gccagcggcc tcatgcggcg cgcttccgca tcctcgaggt ggtcctcatg
    11881 agccgcacca catatgccgc tgtcggtgcc atcggcctcc tcgcgctggc cgcctgcgac
    11941 gatggccccg acgacttcga tgtcagccgg cagatcggcc cggacccggt gctgccagag
    12001 ccgcggcaac ccctcctcgc cagcatcaag ctcgccgagg tggtcggctg gcaggaagga
    12061 cagacgccgg tcgtgccaga gagcctgacg gtcagcgcct acgccaccga cctcgccaac
    12121 ccccggaccg tccacaccct ccccaacggt gacgtgctcg tggtccagtc gcgcgggcca
    12181 tcgggcaagc ctctgccgcg gcccaaggat tatatccgcg gctggatcat gtcgctggcc
    12241 aagggcggcg gcggtgagca gccggagagc aatctcgtca cgctgctgcg ggacaccgac
    12301 cgggacggca tggtggacga gcgcagcgac ctcttgaccg gcctgcattc gccgttcggc
    12361 gtggcctggc acgacgggac gctctacgtc gccgaggccg acgagatcgt cgcctatccg
    12421 tacgagctcg gtgccaccga ggtcggcggg gagccgcaga cgataacacc gctccccggt
    12481 ggccccatca accaccactg gaccaaggat ctcgccctcg gccccgatgc ccgacacctc
    12541 tacgtctcgg tcggctcgaa ctccaacgcg gcggagcgcg ggatggaggc cgagaagggc
    12601 cgcgccgcga tctgggaggt ggaccgggcc accggtgcga gccgggtcta tgcctccggc
    12661 ctgaggaacc ccaacggcct gaccttccac cccgagacgg gcaccctctg gaccgtcgtc
    12721 aacgaacgcg acgagcttgg accgaacctg gtgcccgact acatgacctc ggtgcaggac
    12781 ggcggcttct acggctggcc ctggagctac tacggtgatc acgtcgacgt gcgcgtgcac
    12841 ccgcctcgcc ccgagatggt ggagcaggcg atagcgccgg actacgcgct ctccagccac
    12901 gtggcaccgc tgggcatggc cttcaccatg ggatcggcaa tgcccgaggc gttcgcgaac
    12961 ggcgctttcg tcggcgagca cggcagctgg aaccggacat tcttcaacgg ctacaaggta
    13021 gtctacattc cgttcgagaa cggcatgccg gccgaggacg tggtgacagg cttcctcgac
    13081 ggcgaccaag cccgcggccg ccccgtgggc gtcgccatcg acggcaccgg cgccctgctg
    13141 atcgccgacg acgccggcaa caccgtctgg cgtgtcgccg ctgccgatgg ctcggtcact
    13201 cccgagccca tcggctcgga ccgggtggcg gcggagaggg ccgcaccacc cgatgcggtc
    13261 gggccggcgc cttcaggcga ggcggccacg ccgccgccga ccgaaatcgc gcccgcggtc
    13321 caggagtagc ctaggcgacg cgatccgcat cgattcttcc atccagcgcc tgctcgaggg
    13381 ccaggatgcg ccgctcgagc gcgccgaagc gccggtccag ctcgtccaca tgcatgtagc
    13441 gcggcaccgg ggcctcctgg acgaactcgc gcacccgccg ctcgaactcc agcccgccct
    13501 cgcgcagccg gagcacgagc aggcggacca gctcctcctg agtcgccgtc aacgccaggt
    13561 tcacctgccg cgtcccgggt gagcgccggg cggaagccgg cgcgcgcccg gtttcatcct
    13621 tggatgatgt tgccatgccg catccatact tcggtaatga ttcgcctggc aatcacctct
    13681 tggggacagg gcgaatcgct gctaacgtca ctaggctttt gctaggtttt gtctatcgca
    13741 atgtgatcca gcctgccccg ggagaaggag agccgatgct cacctcgctc tatgtcgtca
    13801 tcatggcgac gctctacatc ggccacacct ggtacctcat cctgctcaag cggcacgccc
    13861 ggatcaggca ggtcgactgg ccgatgcgct gctacgaccg cgcctcgcac ggccgcaact
    13921 tcgtggcgca gtacacaccc ctggcgctca tcctgctcgc catggtcgag atcggcgggg
    13981 cgccggccct cgccatgcac gggctcggca tcatgctggt cgcggcggcc ctgctgcact
    14041 gccattcctt cggctttccg gcccgcaaca gcagcggggt cgccgccatg ctgctggtga
    14101 cctcgatgta cgccatcacc atgaccctgg cgctcgccca gtatgccggg gtcgacgacc
    14161 tgccgaatgc cccgctcatg gcgttgctcg cccacgttcg atgacgccgg cgctcgatgg
    14221 tgatggcgct ctcgcctaca tcctgcgccg gctgcgccag cagggcccgg cgacccacgg
    14281 ccagcatggc tatcacctca ccgtggtcga cctggccgcg cgctatgtgg cggaggtgat
    14341 gggcaagcgg cacccggatc cgatcgagaa cgaggacacc tacgccgcct cgcggccgct
    14401 ctacgacgcc gcctgggaac tcgctcggcg cggcatcctg cgccccagcg tgcacagctc
    14461 cttcatgcag ttcgacgcct accagagcgc cggcggcggc tacacgctca ccgccatcgg
    14521 ggccgaatgg ctgagcgagc tggacgaccg gcagctctcc ttgcgtgccg cctggggccc
    14581 gttcacctga gccggctccc tcccgcttgc gctgagtgat cgcgctggta tcatcagcag
    14641 tcaacgcagg ccgggaggat caatatggac cgaaaggtcc gtccatgctg aagggcggga
    14701 tcatcggctg cggctttttc gccgccaatc atctggcggc gtggcagctg atcgatggtg
    14761 cggcgatcgt ggcggtctgc gaccaggatc cggccagggc gcgagccgcc ggggcggccg
    14821 gcgacggtgc cagggtcttc gacgacgcgg ccaccatgct ggccagcgaa gcgctcgatt
    14881 tcgtcgacat catcaccacg gccccgagcc atcggccgtt ggtcgagctc gccgcacggc
    14941 accgtgtacc cgtgatctgc cagaagccct tcgcccccac cctcgacgac gccagggcga
    15001 tggtcgatgc ctgcgcccaa gccggcgtgc cgctcatggt gcacgagaac ttccgctggc
    15061 aatcgcccct gatggcagtg aaaaatatcg tttcttcggg ggagatcggc gagccggcct
    15121 atgcccggat cagctttcgc cacgcctacg atatctacac caaccagccc tatctgaaga
    15181 ccgagccaca gctcgccatc atggatctcg gcatccacct gctcgatctc gcccgctact
    15241 tcctcggcga ggccacccgg ctgcactgcc gcacccagag ggtcaacccg gccgtcgccg
    15301 gcgaggactg cgtgacgatc ctcctcgacc acgctggcgg tgcggtgagc ctcgtcgact
    15361 tctcgttctt caccaaactc gagccggacc ccttccccca gaccctggtg cggatcgaag
    15421 gcaccaaggg caccgtcgag ctgctcgccg gctatcacct gcgcgtctcg ggcccgggct
    15481 ggtcgcgcga ggaagacgtc gagcccgccg tgcccgcctt cggcgcgaag ccctggcaca
    15541 acatccagga gagcgtcgtc aacatccagc gtcattggat cgagtgcctg cagaacgacc
    15601 gcgagcccgc gacctctggc gccgacaacc tcaagacgat cgacctcgtc ttcagggcct
    15661 acgaatcggc cgcgaccgga agagcctcat gagcgcgctg cgcggcgccg tgatcggctg
    15721 cggcttcttc gcccagaatc acctcaaggc ctggggcgac atcgccggcg ccgagctggt
    15781 cgccgtttgc gatctcgatc cggccaaggc agcggccgcg gcggccttga cgggtgccga
    15841 ggcctttacc gacgtcgacg ccatgctcgc cgcggcgcgg ccggacttcg tcgacatcgc
    15901 gacaaccatg gagacgcacg agccgctggt cgcaaaggcg gcggcggcag gtgccgacat
    15961 catctgccag aagcccttcg cgcccgacat cgctggcgtc cgccgcatcc tccagacagc
    16021 cgaggccgcc ggcatccgct tgatggtgca cgagaacttc cggttccagt cgccgttgat
    16081 cgagctgcgt cgcgccatgc cggcgatcgg cgagccgttc ttcgcccaca tcgcctggcg
    16141 caccggctac gatgtcgtgg ctggccagcc ctatctggca aaggtcgagc gcttcatcct
    16201 gctcgatctc ggcatccatg tcctcgacgt ggcccggttc ctcctgggtg acgtcgccga
    16261 tctctacgcc cgaacgcaaa ggacgatgcc gggtgccagg ggcgaggcac aggcgaccat
    16321 gaccctgcgc catcgctcgg gtgcggtctg cgcggtcacc tgcacctata cgaaccggat
    16381 cgaccccgac cccttcccgc agaccctggc cgagatcgag ggccgggacg gaacgctgcg
    16441 tctcgagcgg gactaccggc tcgagctgca ccgcccgggc agtctcgagg tccgcgacat
    16501 ccgcccgggc gtgccgagct gggccgatcc acaatgggcg ctggcgcagg gctcggtgct
    16561 caacacccag cagcatttcg ttgattgcct gaagtccggc gccgagttcc agacctccgg
    16621 ccgcgacaat ctcaagacct tcgccctggt cgaagccgcc tacgcgtcgg ccgcctccgg
    16681 ccagccggtg gtgcccgagg catgacggag cggatccggg ccagctacct gatcgagacg
    16741 gccgacgacc cgcgccgtgc cgtcgaggtc atggccggcg agcagtcgtc cggcaccttc
    16801 gtggccctgc cgggcgaaac gcccgagctc aaggagcgct acggcgcccg gatcgagaag
    16861 ctggagctgc tggaggaggc cgcaaccccg tccctgcccg gtgcgaaggc tggcccggtc
    16921 taccgccgct gcgccgccga gctctcctgg ccactcgaga atctcggcgc ctcgctaccc
    16981 aacctcgtcg ccacggtcgc cggcaatctc ttcgagctgc accagttctc gggcctgcgc
    17041 ctcctcgaca tcgacctgcc gccggccttc gccgggcgct acccggggcc gcagttcggc
    17101 attgaaggga cgcggcgcct tgccggcgtc gccagcggcc ccctgatcgg caccatcatc
    17161 aagccgtcgg tcggtctctc gcccgaggcg acggcggaga tggtcgacgc cctctgccgg
    17221 ggcggcatcg atttcatcaa ggacgacgag ctgcaggcgg acgggccgca ctgcccgttc
    17281 gaggcccgcg cccgggcggt gatgcaggtc atcgaccgcc
//
LOCUS       DAOTFI010000101        17043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4050_length_17043_cov_7.244643, whole genome shotgun sequence.
ACCESSION   DAOTFI010000101 DAOTFI010000000
VERSION     DAOTFI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17043
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4050_length_17043_cov_7.244643"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="P5558_11780"
     CDS             complement(<1..307)
                     /locus_tag="P5558_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY25036.1"
                     /translation="MREQIKAVATELLITHGVRGLRFGDIADRLDITRANIHYHFGTK
                     KNLVEEVIDEYVQAAIAGLGEVWSDPGLTYQEKALRTMEFNRGRYTKFNTGSRTGRSW
                     "
     gene            465..1226
                     /locus_tag="P5558_11785"
     CDS             465..1226
                     /locus_tag="P5558_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY25037.1"
                     /translation="MRLKDKVCLVTGGGSGIGRATCELFAREGARLVVVDRDEASAAA
                     TAEACAGPGEAPLVLRADVAKSADSRGMIEATVQHFGRLDVLVNNAGYGILGTVVETS
                     EDEWNALMAVNVTGVFLACHNAIPLMARQGGGAIVNVASVVSSIGIPNRAAYCASKGA
                     VASLTRAMALDHVADGIRVNAVAPGTIRSAYFDALLAKADDPEGLLAHLEGRQPFKRM
                     GSPAEIATGILYLASDESSFCTGTILTIDGGMTAQ"
     gene            1248..2018
                     /locus_tag="P5558_11790"
     CDS             1248..2018
                     /locus_tag="P5558_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY25038.1"
                     /translation="MYDLDERIALVTGAASGIGRAIALRLAQEGCHVGLVDTAEAGLR
                     ETARSIEALGRKTCLVVADVTERHNVDRAVARLTETLGTADILVNSAGILRVGAMLDQ
                     PYEDFARQFRVNVDGTFHVCQAVVPAMVAKGRGAVINLSSWLGKKGMKLYGGYAASKF
                     AVIGMTQALALEVAAFGVRVNALCPGLIVETGMRDQAEALHRSLGLPSAEERVSTIPR
                     GRLGKPDDIARVAAFLASDEADFMTGQAVNVTGGFWLN"
     gene            2055..3020
                     /locus_tag="P5558_11795"
     CDS             2055..3020
                     /locus_tag="P5558_11795"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY25039.1"
                     /translation="MRQFKGYRPKGVIPATLLAFDDDFSINEQESRRHLAEVAATKGL
                     NAITVNGHASEVHACTFEEQKRILDISLEEVGDRLPLINGIYADGSLEAARLARMAEA
                     AGAAALLVFPPQSMSMGGQLRPEMALAHFKTIAAATDLPLIVFQYPQATGLGYPFETL
                     LRMCEEVPSIVAIKDWSNDGLLHERHTRTLQSLARPVTVLTTHSGWLMASLTMGCGGL
                     LSGAGSVIPELQVALFEAVQAKDLATAQAINDRIYPMVQAFYAPPFLDMHNRMKECLV
                     MLGRLDKAVVRPPLMKLGQAELDRLRQALDAAGLGRAGAFAAAAE"
     gene            3090..4289
                     /locus_tag="P5558_11800"
     CDS             3090..4289
                     /locus_tag="P5558_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRY25040.1"
                     /translation="MLDLLDGVRVVSFNHFLLGPVGMQILGDLGADVIAVEPIEGAFQ
                     RRFAGAGTWVDGQSSLYLTGNRNKRNLAVDLKSEAGRAVVLRLAGKADVVSENFRPGV
                     MEKLGLGYEALKEQNPSLVYAAASGFGANGPYAKRPGQDMLIQAMSGLAKITGEASTG
                     PRTVGVSAADHHGAALLALGVVAALFRRGRTGKGCRVDVNLLSAAIDLQMESFVCYLN
                     ATRKHDVTAPHRVGGWYYTAPYGIYPVKGDHVAISLTTMQALGAALDSPELAAIPEEE
                     AAARGDEIVAALNRILPTRTFAEWQPRLEEHDIWHAKVNDYDEVQEDPQVRHNESFLT
                     VEGATGAPIKVVNHAIRYDGAAASVTLPPQPLGAQTRDILAEIGYGATEIEGLIASGA
                     VRAAERR"
     gene            4286..5080
                     /locus_tag="P5558_11805"
     CDS             4286..5080
                     /locus_tag="P5558_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY25041.1"
                     /translation="MIADLDIDLDGRRLAARRIGDWGRDEPVLVFLHEGLGSISMWRD
                     FPEALAARTGLPALIYDRFGYGRSSPAPTPWPPDFLRNEATEVLPALLAETGIGRPLV
                     VGHSDGGTIALMFAAAFPDRPLACITLAAHVVLEARTKAGLGALAERWQDDADFRRRL
                     SRHHDSADALVRAWLEVWNGHLPTGWNMLDDLARITCPVLAIQGELDEHGTLAQVDEI
                     TSRVAGPAEAAILAGCGHVPHLEQLEEVLAHIADFIAAFATRRSNG"
     gene            5176..5418
                     /locus_tag="P5558_11810"
     CDS             5176..5418
                     /locus_tag="P5558_11810"
                     /inference="COORDINATES: protein motif:HMM:NF020542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HRY25042.1"
                     /translation="MPETSRVLSVRAKWDPEAGVWVALSDDVPGLVAEAASLDELFSE
                     LQELIPSLLELNGVKDGKSAPFQLVAASSRAGMAAA"
     gene            5418..5612
                     /locus_tag="P5558_11815"
     CDS             5418..5612
                     /locus_tag="P5558_11815"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRY25043.1"
                     /translation="MAHYGSEVREILKKNGCYLHRQGKGSHEIWCNPASSKYFAVPTK
                     ILSRHTANEILKQAGLQKAF"
     gene            5653..6402
                     /locus_tag="P5558_11820"
     CDS             5653..6402
                     /locus_tag="P5558_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY25044.1"
                     /translation="MNNRKILVTGSGSGIGAAIARRLAGEGARIMLHARENRAGCEAV
                     ARELRERGAEVAIQLGDLQDPATPEALVDATVERFGGLDVLVANAGFPVLEQLGELTR
                     ADLDRCYAVIQAGLFQMATRAMPELQRSAAGRIVAISTGNAHVFRPNYPIYPASAAAK
                     AAAEVLVRCLAVQLAPFGVTANGVVPGLIGKDDGVLGHHAAEEVPAFLEHIPMGRIGR
                     PDEVAAMVGFLCSPDAGYVTGQIIHVNGGLI"
     gene            6510..7499
                     /locus_tag="P5558_11825"
     CDS             6510..7499
                     /locus_tag="P5558_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY25045.1"
                     /translation="MSFHTRIMGAALAGATLAFATAASAETLRLGHDQPEAHGYQEVG
                     VYLDKRLKEELGDDWAVTIFPGAQLGNEMAMLDSVIAGNLDMSIAASANASTFIPELG
                     LFSVSYVFDDVDHFRRVLNDAEFNAMIDEIIASKELGIKRLGTFTAGVRNVYNREKPI
                     TSLDDLASMKMRVMASPVESKVWGELGTLPVAIPFGDVYTGMQTGLVQAAENAAAVYA
                     SNKHYEVAPYYSLTGHQWLMANLYINQEIFDGLPPEAQAALVEVGAELTDHIIDYVVA
                     SDAAFVEKMQAEYGVQVNEVDTTPFVERLAATQDAVAAELGVENVLDRIRALK"
     gene            7553..8563
                     /locus_tag="P5558_11830"
     CDS             7553..8563
                     /locus_tag="P5558_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY25046.1"
                     /translation="MQNPTMRGVLGAAATATAMALVMGWSFDAQAQKVLRLGHDQVTA
                     HTYQHTSEFFAERVKELSNGELEVQIFPGATLGTETSMLQEVISGNLDMSLSTTANAS
                     SFVPAFGMLSVSYLFSDGEHFKRALLDPEFNAALDALIEAQDPGFIRVASITPGARSV
                     YNIFGPVEALDDIEGKKMRVMASPVESKVWGQLGTLPVAIPFGDVYTGMQTGLVEAAE
                     NSPGSYVLNKHYEVAPYFSLTEHQWPVSLIFMGETTWEGLSEAEREVILEAGRDTAAF
                     GVDNATSGDNALLDEMASKYGVKVNRLDTAPFVEQLAALQDETAAELGTEELLARIRE
                     LR"
     gene            8617..9162
                     /locus_tag="P5558_11835"
     CDS             8617..9162
                     /locus_tag="P5558_11835"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY25047.1"
                     /translation="MSGPPEPTTWPVYARLCRLNAQLEQVLSLAAGACLALFTVVVAI
                     DVIYRQVLAQPMMWPSEWSVMAFVWSVMLGAAVSASRQSHFVVVLVADRGRAGDHVLR
                     TFVALAAVLFSLVLVYFGWRMMMTGTRRFTPMMGYPMTYVFAAFPVAGIAFLLFSVEQ
                     LIAAVRRYPPRHAPHTEIGSE"
     gene            9159..10454
                     /locus_tag="P5558_11840"
     CDS             9159..10454
                     /locus_tag="P5558_11840"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY25048.1"
                     /translation="MNPITLLFVSFFVMIALRVPVAYALALASLLIIVQEGLRADLVV
                     QQMFQGLNSFTLLAVPFFLLAGQLLNSGLITDRLMRLAEVMVGWIRGGLAHINVVVSM
                     IFAGMSGSSTADTAGVGSVIIPAMYKRGYDRRFTVAITAASSTMGSIIPPSIMMVVYG
                     AQGGVSIGGLFLAGAIPGMLIGLGMMIYSYVYAIRRGLPAEARPTVREFAIAWKEGLL
                     PLLVPVIIIGGVLTGKFTATESGMVATVYVLFLMFVFYRSARMRELPKILAEAAVLYS
                     QPLLAVAAASVFGWLLAYFQAPQMIIGLAGDMTGSIPLTLLSIMVVFIIVGTFMDAIP
                     AIIIFMPIVTKLLETSGANAIHGGIIVVMTLALGLITPPYGLCLLLASAIGRVPVVKV
                     LPHIMAFYAIIIGIIITIIFVPEIALFLPRLVMPQFVGS"
     gene            complement(10463..10906)
                     /locus_tag="P5558_11845"
     CDS             complement(10463..10906)
                     /locus_tag="P5558_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRY25049.1"
                     /translation="MTVIPAEIGAEVRFSKTLGETDFYLFSGISGDFDKVHVNEEYMK
                     TTVFGRRIAHGILVLALSSNTASMMSSRYTTANSPFTPVSAGYDKVRFLKPVFIGDTI
                     TVSYRVAAVEQERLRSRSRVEMTNQKGETVLVAEHILAWVPRETG"
     gene            complement(10903..12630)
                     /locus_tag="P5558_11850"
     CDS             complement(10903..12630)
                     /locus_tag="P5558_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25050.1"
                     /translation="MKQLFTPASIGRVEIKNRIVLPSMTTRLADEAGGVTEATLAYYL
                     ARATGGVGLVTVEMAAPEPAGRHRRRELGIYDDRFLPGLRRLTTALREAGARTTIQLG
                     HGGGHTRDDICGEVPIAPSAVPHDVFEITFATIVPEAMTAERIEQTTRAYVAAAVRAE
                     AAGFDMVEIHAAHGYLISQFHTPAENLRQDRYGGSLENRARFGLDILRRIKAELPDFP
                     MIYRLTVDDFFEGGLTFEEGRQIALWAAEAGADAIHVTAGHYRSKPSAMRMTPPMAEP
                     DAPFLDFARAIRREVDVPVIAVGRLGDPAVAIAAVEDGACDFVALGRPLLADPDWVAK
                     AEQGEPVRRCIACNTCVNEMRGGAALGCLVNARAGREAELPEVSPIRGERIAVVGAGP
                     AGLSFAGLVAGANEVTVFERDAECGGALLQAALAPTFQDVEAAKGPIGAYVAGLEAAL
                     RRQGVRLELGVDVLAVPERLRGFDRVVIASGAHYRFGLGPLVGFLLERGFGRTALARR
                     LFAAPGLRDWLYHRARQPSGAAIRAAIGAGPRIDVIGDAGRAGKTKPAITSAFAAALR
                     PESHEEITP"
     gene            complement(12638..13789)
                     /gene="ugpC"
                     /locus_tag="P5558_11855"
     CDS             complement(12638..13789)
                     /gene="ugpC"
                     /locus_tag="P5558_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY25051.1"
                     /translation="MATIRLEKVEKRFGDVVAVAGIDLAIADGEFVVLVGPSGCGKST
                     TLRMVAGLEPVSGGKIKFDQYVVNNLTPKNRDIAMVFQSYALYPHMTVFDNMSFGLRL
                     KGMGRDEIRRRVQDAAALLNITELLRRKPRELSGGQRQRVAMGRAIVRQPKAFLFDEP
                     LSNLDASLRAQMRVEIKRLHKRLETTVIYVTHDQIEAMTLADRIVIMRGGQIVQVGAP
                     MEVYEQPVDRFVAGFIGAPQMNFLPGRVVAEGNGLAFAIEGGILLPLPAARDAAYAAW
                     RDRPMELGVRPETLHVGRFEGPDAATFEAAIDVVEPMGNEGLAFFQVAGVEVAATCDP
                     YLRRDQGDRVPLTVDMARAHLIDVETGKVVPSGTAGRQAGHPAPALAAS"
     gene            complement(13829..14662)
                     /locus_tag="P5558_11860"
     CDS             complement(13829..14662)
                     /locus_tag="P5558_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25052.1"
                     /translation="MNPRAALFRLWVYGLAALLLVICAFPLAWMVLTSIKPDREILTA
                     VPTFIPSELTIDAYRRLFTSTIFATYFRNSIIVAGLATLLTVAVGTLAAYGITRFRFR
                     GRELISTTMLFTYMFAPIMIVVPFYILMRQLGLVNSLFGLVLSYATFSLPFSMWLLRS
                     FFQSIPLDLEEAAMTDGASRPQAVVRVIVPLALPGVIAVTIFTFIVAWNDYLFARVLI
                     SSDNLKTLPVGINDIYVSTVTDWGMMMAAGVVITLPALIFFVLVQRYLIAGWGAGGVK
                     G"
     gene            complement(14670..15614)
                     /locus_tag="P5558_11865"
     CDS             complement(14670..15614)
                     /locus_tag="P5558_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25053.1"
                     /translation="MAVATQDGSTLAADNGEAEVWLRGRRIPEHWWGYVLVAPAMLLV
                     LGLILYPVIYSFWLSVHSKHAYLPIETFVGLDNFRYFLTDFDGFWRGLWLGTVYAFGT
                     VALQLVFGVGAALLLNQTFIGRGFVRGCALFPYMVPTIVVVILMRWILNDNYGVLPYA
                     LRLVGLEPGLMLGPESIMLSLILISTWTYFPFIVIGTLARLQTIDPELYGAAKVDGAG
                     PLRRFWHITLPQIRSVLFVLVLLRFLFMFTKFDIIWLFASGTGVGYFTRTLPIYTYVK
                     TFQELQVGMGAALSVVIFLILVAFALLYFRLFKRANEP"
     gene            complement(15676..>17043)
                     /locus_tag="P5558_11870"
     CDS             complement(15676..>17043)
                     /locus_tag="P5558_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25054.1"
                     /translation="ESRRYGYRTLLAGATLALLLPTALAAQERVIQVWHTEPNPATKA
                     VMDEIIADFIETHPDVRIEHEAVGWGDLDLKMQAALAAGAPPTLTQAQTYVERSLAAK
                     GMLMPLNDVFDSIGRDDIFDVINFIHTYDDGNNYGISHAVGADTIIIRKDFYREAGLD
                     PDTAPATWDEWLEQLKALTIDSDGDGRMDRYGLGLAGPSFFINEDLYMWVGSNGGRLF
                     DEVGRPTFTEPQVIETLEFWKELNDCCLSPDWLSQSYLDTFANLATGQVASILGWGRG
                     TGYFEQYAPDAVAAGDIAVFDSKPAGPSGEPGKFVTQFDNESWIVFKDADNADVAVEF
                     LEFFYEPANYLKYVESVPTHLLPIRKSIAADPAYLANPAFEKWEDWVQAQLKIIAEDD
                     PRPLLMTQWEDLKLPFTAEIAGSGILADMVTDVVQGIRTPMEAAERAQGRAEELITQL
                     GYRKW"
BASE COUNT         2775 a         5900 c         5648 g         2720 t
ORIGIN      
        1 tccaggatcg gcccgtgcgg ctgcccgtgt tgaacttcgt gtagcggccg cggttgaact
       61 ccatcgtccg cagcgccttc tcctgatagg tcaggcccgg atccgaccag acctcgccga
      121 ggccggcgat tgccgcctgc acatattcgt cgatcacctc ctcgacgaga ttcttcttgg
      181 tgccgaagtg atagtggatg ttcgcacggg taatatcgag ccgatcggcg atgtcgccga
      241 agcgcaggcc gcgcacgcca tgggtgatca gaagctcggt ggccaccgcc ttgatctgct
      301 cgcgcatgtg cgttccaatc cggctcgccg cccggggaag caggcttgcg ggccgcgccc
      361 ggggtcgcta tcttactagt cagtcagcga cactctagac catttgatgg tttgtcgcac
      421 ctatcttgtt ccggcgctgc cagccggcgg aggcttcaaa cttcatgcgg ttgaaggaca
      481 aggtctgcct cgtgaccggc ggcggctcgg gcatcggccg tgccacctgc gagctcttcg
      541 ctcgcgaagg ggcgcggctc gtcgtcgtcg acagggacga ggcgagcgct gcggcgacag
      601 ccgaggcttg cgccggcccg ggcgaggcac ctctggtgct ccgggccgac gtcgcgaaat
      661 ccgccgattc caggggcatg atcgaggcca ccgtgcagca cttcggccgc ctcgacgtgc
      721 tcgtcaacaa tgccggctat ggcatcctcg gcaccgtcgt cgagaccagc gaggacgaat
      781 ggaacgcgct catggcggtc aacgtcaccg gcgtcttcct cgcctgccac aacgcgatcc
      841 ccctcatggc ccgccagggc ggcggggcca tcgtcaacgt cgcctccgtc gtctcgagca
      901 tcggcattcc caaccgggcc gcctattgcg cgtccaaggg tgccgtcgcc tccctgaccc
      961 gggccatggc cctcgaccat gtggccgacg ggatccgcgt caacgccgtc gccccgggca
     1021 ccatccggtc ggcctatttc gatgcgcttc tggccaaggc cgacgacccg gaagggctgc
     1081 tcgcccatct ggaggggcgc cagccgttca agcgcatggg atcaccggcc gagatcgcga
     1141 ccggcatcct ctatctggcc tctgacgaat cctccttctg cacgggcacc atcctcacca
     1201 tcgacggcgg catgaccgcc caatgacagc gaatcgggga gccttccttg tacgatctcg
     1261 atgagcgcat cgctctcgtc accggtgcgg cctccggcat cggccgcgcc atagcgttgc
     1321 ggctggcgca ggaaggctgc catgtcggcc tcgtggatac cgccgaggcg ggattgcggg
     1381 agactgcccg gagcatcgag gcgctcggcc gcaagacctg cctggtcgtc gccgacgtca
     1441 ccgagcgtca caatgtcgat cgcgccgtcg ccaggctgac cgagaccctc ggcactgccg
     1501 acatcctcgt caactcggcc ggcatcctgc gcgtcggcgc catgctcgac cagccttacg
     1561 aggacttcgc ccggcagttc cgggtcaatg tcgacggcac gttccacgtc tgccaggccg
     1621 tcgtcccggc gatggtcgcc aagggcaggg gggccgtgat caacctctcc tcctggctcg
     1681 gcaagaaggg catgaagctg tatggtggct atgctgcgtc gaagttcgcg gtgatcggca
     1741 tgacccaagc cctggcgctc gaggtcgccg cctttggggt gcgggtgaac gcgctctgcc
     1801 ccgggctcat cgtcgagacc gggatgcgcg accaggccga ggcgctgcac cgctcgctgg
     1861 gcctgccgag cgccgaggag cgggtctcca ccatcccgcg cggccggctc ggcaagccgg
     1921 acgatatcgc ccgggtcgcc gccttcctgg cttccgacga ggccgacttc atgaccggcc
     1981 aggcggtcaa cgtgacgggt ggattctggc tcaattgagc tatctcgaat cgaacagcag
     2041 gggagcttgc agccatgcga cagttcaagg gctaccggcc gaagggcgtc atcccggcga
     2101 cgctcctggc cttcgacgac gatttctcga tcaacgagca ggaatcgcgc cggcaccttg
     2161 ccgaggtggc ggcgacgaag ggcctgaacg cgatcacggt caacggccat gcgtccgagg
     2221 tgcatgcctg caccttcgag gagcagaagc gcatcctcga catctcgctc gaggaggtcg
     2281 gcgaccggct gccgctgatc aacggcatct atgccgacgg cagcctcgag gccgcaaggc
     2341 tcgcccggat ggccgaggcc gccggggccg cagcactgct cgtcttcccg ccgcagagca
     2401 tgagcatggg cggccagctc cgcccggaaa tggcgctcgc ccacttcaag acgatcgccg
     2461 ccgccaccga cctgccgctc atcgtgttcc agtacccgca ggcgacgggc ctcggctacc
     2521 cgttcgagac gctgctccgc atgtgcgagg aggtgccgtc catcgtcgcc atcaaggact
     2581 ggtcgaacga cggcctgctg cacgagcggc acacccgcac cctgcagtcc ctcgcccggc
     2641 cggtcaccgt gctcaccacc cacagcggct ggctgatggc gtccctgact atgggctgcg
     2701 gggggctgct ctccggtgcg ggcagcgtca tcccggagct gcaggtcgcc ctcttcgagg
     2761 cggtccaggc caaggatctg gcgacggccc aagcgatcaa cgaccggatc tacccgatgg
     2821 tgcaggcctt ctacgcgccg cccttcctcg acatgcacaa ccgcatgaag gaatgcctcg
     2881 tcatgctcgg ccggctcgac aaggcggtgg tccggccgcc gctgatgaag cttggccagg
     2941 cggagctcga ccggctgcgc caggcgctgg acgccgccgg cctcggccgg gcaggggcct
     3001 tcgccgcggc ggccgaataa ccgaacgcaa cgacgcgtca ggcggccatc gcggtcgcct
     3061 gacgcgccaa tctcaccggg tgggacgcga tgctcgatct tttggacggc gtgcgcgtcg
     3121 tcagcttcaa ccatttcctg ctcggcccgg tcggcatgca gatcctgggt gatctcggcg
     3181 ccgacgtgat cgccgtggag cccatcgagg gcgccttcca acgccgcttc gccggggccg
     3241 gcacctgggt cgacggccag tcctccctct atctcaccgg caaccgcaac aagcgcaacc
     3301 tcgccgtcga cctcaagtcc gaggcgggcc gcgccgtggt cctgcgcctc gccgggaaag
     3361 ccgacgtggt cagcgagaac ttccgcccgg gggtcatgga gaagctcggc ctcggctacg
     3421 aggcgctgaa ggagcagaac ccgtccctcg tctacgccgc cgcttccggc ttcggcgcca
     3481 acgggcctta tgccaagcgg ccgggccagg acatgctgat ccaggccatg tccgggctcg
     3541 cgaagatcac cggcgaggcc tcgaccgggc cccggacggt cggcgtctcg gccgccgacc
     3601 accatggcgc tgccctcctc gccctcggcg tcgtcgccgc cctcttccgg cgcggccgca
     3661 ccggcaaggg ctgccgggtc gacgtcaacc tcctctcggc cgccatcgac ctgcagatgg
     3721 aatccttcgt ctgctacctg aacgccacga ggaagcacga cgtcacggca ccgcaccggg
     3781 tcggcggctg gtactacacg gccccctacg gcatctatcc ggtcaagggc gaccacgtcg
     3841 ccatctcgct caccaccatg caggcgctcg gagccgccct cgattcgccc gagctcgccg
     3901 ccatccccga ggaggaggcg gccgcgcgcg gcgacgagat cgtggccgcc ctgaaccgca
     3961 tcctgccgac gcggaccttc gccgagtggc agccccgtct cgaggagcac gacatctggc
     4021 acgccaaggt caacgactac gacgaggtcc aggaggaccc gcaggtccgc cacaacgaga
     4081 gcttcctcac cgtcgagggt gccaccggtg cccccatcaa ggtggtcaac cacgccattc
     4141 gctacgacgg tgccgccgcc tcggtgaccc tgccgccgca gcccttgggc gcccagaccc
     4201 gcgatatcct ggccgagatt ggctacggtg ctacggagat cgaggggctg atcgcgagcg
     4261 gcgcggtccg ggctgccgag cgtcgttgat cgccgacctc gacatcgatc tggacggccg
     4321 gcgcctcgcc gcgcgccgca tcggcgactg gggccgggac gagcccgtgc tggtcttcct
     4381 ccacgagggc ctcggcagca tctcgatgtg gcgcgacttc cccgaagcgc tcgccgcccg
     4441 gaccggcctg cccgccctga tctacgaccg cttcggctac ggccggtcca gcccggcccc
     4501 gaccccctgg ccaccggatt tcctccggaa cgaggccacg gaggtgctgc cggcactcct
     4561 cgccgagacg ggcatcggcc gccccctggt cgtcggccac agcgacggcg gcacgatagc
     4621 gctcatgttc gccgccgcct tcccggatcg tccgctcgcc tgcatcaccc ttgcggccca
     4681 cgtcgtcctc gaggcgcgga ccaaggccgg cttgggtgcc ctcgccgagc gctggcagga
     4741 cgatgccgac ttccgccgcc gcctttcccg ccatcacgac tcggccgatg ctctggtccg
     4801 ggcctggctc gaggtctgga acggccacct gccgaccggc tggaacatgc tggacgacct
     4861 cgcccgcatc acctgcccgg tcctcgccat ccagggcgag ctcgacgagc acggcacgct
     4921 ggcccaggtc gacgagatca ccagccgcgt cgccggcccg gccgaagccg ccatcctggc
     4981 cggatgcggc cacgtcccgc atctggagca gctggaagaa gtgctcgccc acatcgccga
     5041 ttttatcgcc gctttcgcga caagaaggtc gaacgggtag gcagtcgttc gtatgacgtg
     5101 gcaacaagag gaagcttgtc aggcaccttg cctcgattga tatgtatccg atcgaaaggg
     5161 gaacgtgaga agcacatgcc ggaaaccagc cgcgttctga gcgttcgagc gaaatgggac
     5221 cccgaagccg gggtctgggt ggcgctgagc gacgatgtcc cgggtcttgt cgccgaggcc
     5281 gcaagcctcg acgaactctt cagcgagctt caggaactga tcccgtcact tctggagctg
     5341 aacggggtca aggacggcaa gtcggcgccc ttccagcttg tcgcagccag cagccgagcg
     5401 ggaatggcag ccgcatagtg gcccactacg gcagtgaggt ccgcgagatc ctgaagaaga
     5461 atggttgtta cctgcacagg cagggcaagg gctcgcatga aatctggtgc aatcctgcat
     5521 cgagcaagta tttcgcggtg cccaccaaaa tcctgtcgcg gcacacggcc aacgagattt
     5581 tgaagcaggc cggcttgcag aaggcatttt agcttgcccg ccaccgttgc gggcagccag
     5641 ggaacgtcag gaatgaacaa cagaaaaatc ctcgtcaccg gctcgggcag cggtatcggg
     5701 gcggccatcg cccgccggct ggccggcgag ggcgcccgca tcatgctcca tgcgcgggag
     5761 aaccgggcgg gctgcgaggc cgtcgccagg gagctgcgcg agcggggggc ggaggtcgcg
     5821 atccagctcg gcgacctgca ggacccggcg accccggaag cgctcgtcga cgccacggtg
     5881 gagcgcttcg gcgggctcga cgtgctcgtg gccaatgcgg ggtttccggt gctcgagcag
     5941 ctcggcgagc tgacccgggc cgatctcgac cgctgctatg ccgtcatcca ggcgggcctc
     6001 ttccagatgg cgacccgcgc catgccagag ctgcagcgct cggccgccgg gcggatcgtc
     6061 gcgatctcca ccggcaacgc ccacgtcttt cgccccaact acccgatcta tccggcctcc
     6121 gccgcggcca aggcggcggc cgaggtgctg gtccgctgcc tcgccgtcca gctcgcaccc
     6181 ttcggcgtca ccgccaacgg cgtcgtcccg gggctgatcg gcaaggatga tggcgtgctc
     6241 ggccaccatg cagcggagga ggtgccggct ttcctcgagc acatcccgat gggccgcatc
     6301 ggccggcccg acgaggtggc tgccatggtg ggcttcctct gctcgccgga tgccggctac
     6361 gtcaccggcc agatcatcca cgtcaatggc ggtctgatct gaggtcggac gaaacgactt
     6421 tatttccgtg cgaccctgtg gcaagctgac ctccaagtaa gtaagtggtt ggcaacacag
     6481 gccaacacca tccgggaggg caagcggcaa tgagctttca cacgcggatc atgggggcgg
     6541 cgctggcagg cgccacgctg gctttcgcca cggctgcctc ggccgagacc ctgcggctcg
     6601 ggcacgacca gccggaagcg cacggctacc aggaggtcgg cgtctatctc gacaagcgcc
     6661 tcaaggagga gctcggcgac gactgggccg tgaccatctt tcctggcgcc cagctcggca
     6721 acgagatggc gatgctcgac agcgtcatcg ccggcaatct cgacatgtcg atcgcggcct
     6781 ccgccaacgc ctcgaccttc attcccgagc tcggcctctt cagcgtcagc tacgtgttcg
     6841 acgatgtcga ccacttccgc cgggtgctca acgacgccga gttcaacgcg atgatcgacg
     6901 agatcatcgc gtccaaggag ctcggcatca agcggctcgg caccttcacc gccggcgtgc
     6961 gcaacgtcta caaccgcgag aagccgatca cctcgctcga cgacctcgcc agcatgaaga
     7021 tgcgggtcat ggcctcgccg gtggaatcca aggtctgggg cgagctcggc accctgccgg
     7081 tcgcgatccc cttcggcgac gtctacaccg gcatgcagac cggcctcgtc caggccgccg
     7141 agaacgcggc tgcggtctac gcctcgaaca agcactacga ggtcgcaccg tactattcgc
     7201 tgaccggcca ccagtggctg atggccaatc tctacatcaa ccaggagatc ttcgacggcc
     7261 ttccgccgga ggcgcaggcg gcgctggtcg aggtcggcgc cgagctgacc gaccacatca
     7321 tcgactacgt ggtcgcgagc gatgccgcct tcgtcgagaa gatgcaggcc gagtacggcg
     7381 tgcaggtgaa cgaggtggac acgaccccct tcgtcgagcg cctcgccgcc acccaggacg
     7441 cggtggcggc cgagctcggc gtcgagaacg tgctcgaccg catccgcgcg ctgaagtagc
     7501 ccaaatgctc ggggcggcct ccagcccgga ggtcgccccc aaggaggaca cgatgcagaa
     7561 tccgacgatg aggggcgtgc tcggggccgc ggccacggcg accgccatgg cgctggtcat
     7621 gggctggtcc ttcgatgccc aggcgcagaa ggtgctgcgg ctcggccacg atcaggtcac
     7681 cgcccacacc taccagcaca cctcggagtt cttcgccgag cgggtgaagg agctcagcaa
     7741 cggcgagctc gaggtgcaga tcttcccggg cgccacgctc ggcaccgaga cgtcgatgct
     7801 gcaggaggtc atctccggca atctcgacat gtcgctctcg accacggcca acgcctcgag
     7861 cttcgtgccg gccttcggca tgctcagcgt cagctacctc ttcagcgacg gcgagcattt
     7921 caagcgcgcg ctcctcgatc ccgagttcaa cgccgccctg gacgcgctga tcgaggccca
     7981 ggaccccggc ttcatccggg tcgccagcat cacgccgggc gcgcgcagcg tctacaacat
     8041 cttcggcccg gtcgaggcgc tcgacgacat cgagggcaag aagatgcggg tcatggcctc
     8101 gccggtcgaa tccaaggtct ggggccagct gggcaccctg ccggtcgcca ttcccttcgg
     8161 cgatgtctac accggcatgc agacgggcct cgtcgaggcg gcggagaact cgcccggctc
     8221 ctacgtgctg aacaagcact acgaggtcgc cccctacttc tcgctgaccg agcaccagtg
     8281 gcccgtctcc ctgatcttca tgggcgagac gacctgggag gggctgagcg aggccgagcg
     8341 cgaggtcatc ctcgaggccg gccgcgacac ggcggcgttc ggcgtcgaca acgccacctc
     8401 gggcgacaac gcgctgctcg acgagatggc gagcaagtac ggcgtcaagg tcaaccgcct
     8461 ggacacggca cccttcgtcg agcagctcgc cgccctgcag gacgagacgg ctgccgagct
     8521 cggcaccgag gagctcctgg cacgcatccg cgagctgcgc tgaatgaccg atagcgaccg
     8581 ccggccgggg cgccgatccg tgtcccggcc ggccgcatga gcggcccacc cgagccgacg
     8641 acctggcccg tctacgcgcg gctctgccgg ctgaacgcgc agctcgagca ggtgctgtcg
     8701 ctcgccgccg gcgcttgcct cgccctcttc accgtggtcg tcgcgatcga cgtgatctac
     8761 cgccaggtgc tggcccagcc gatgatgtgg ccgtcggaat ggtcggtcat ggccttcgtc
     8821 tggtcggtga tgctgggggc cgccgtctcc gccagccggc agtcgcactt cgtcgtcgtg
     8881 ctggtcgccg accggggacg ggccggcgac catgtgctgc gcaccttcgt ggcgctcgcc
     8941 gccgtgctct tctcgctcgt gctcgtctat ttcggctggc gcatgatgat gaccgggacg
     9001 cgccgcttca ccccgatgat ggggtatccc atgacctacg tgttcgccgc cttcccggtg
     9061 gccggcatcg ccttcctgct cttctccgtc gagcagctca tcgccgccgt gcgccgctac
     9121 cccccccgcc acgcgccgca caccgagatc ggcagcgaat gaacccgatc accctcctct
     9181 tcgtcagctt cttcgtgatg atcgcgctgc gcgtgcccgt cgcctacgcg ctcgccctgg
     9241 cgtcgctgct gatcatcgtc caggaggggc tgcgcgccga cctcgtcgtg cagcagatgt
     9301 tccagggcct gaattcgttc acgctcctcg ccgtgccctt cttcctgctc gccggccagc
     9361 tcctcaacag cggcctcatc accgaccggc tgatgcgtct cgccgaggtg atggtgggct
     9421 ggatcagggg cggcctcgcg cacatcaacg tcgtggtctc gatgatcttc gccggcatgt
     9481 cgggctcgtc caccgccgac accgccggcg tcgggtcggt catcatcccg gccatgtaca
     9541 agcgcggcta cgatcgccgc ttcaccgtgg ccatcaccgc cgcgtcgtcg accatgggct
     9601 cgatcatccc gcccagcatc atgatggtgg tctatggcgc gcagggcggc gtctcgatcg
     9661 gcgggctctt cctcgccggc gccatcccgg gcatgctgat cggcctcggc atgatgatct
     9721 acagctacgt ctacgccatc cgccgtggcc tgccggccga ggccaggccg acggtccgcg
     9781 agttcgccat cgcctggaag gagggcctgc tgccgctcct agtcccggtg atcattatcg
     9841 gcggcgtcct caccggcaag ttcacggcga ccgaatccgg catggtcgcc accgtctacg
     9901 tgctcttctt gatgttcgtc ttctatcgct cggcccggat gcgggagctg ccgaagatcc
     9961 tcgccgaggc cgccgtgctc tactcgcagc cgctcctcgc cgtcgccgcc gccagcgtct
    10021 tcggctggct gctcgcctac ttccaggcgc cgcagatgat catcggcctc gccggcgaca
    10081 tgaccggcag catccccctg acgctcctct cgatcatggt ggttttcatc atcgtcggca
    10141 ccttcatgga cgcgattccc gcgatcatca tcttcatgcc gatcgtcacc aagctgctcg
    10201 agaccagcgg cgccaacgcg atccatggcg gcatcatcgt cgtcatgacc ctggccttgg
    10261 gcctgatcac cccgccctac ggcctctgcc tgctcctggc atccgccatc ggccgggtgc
    10321 cggtggtcaa ggtgctgccg cacatcatgg ccttctacgc catcatcatc ggcatcatca
    10381 tcaccatcat ctttgttccg gagatcgccc tcttcctgcc gcgcctggtc atgccgcaat
    10441 tcgttggcag ctgagcggcg cctcagccgg tttcgcgcgg cacccaggcg aggatgtgct
    10501 cggccaccag cacggtctcg cctttctggt tggtcatctc gacccgactc ctggagcgca
    10561 ggcgctcctg ctcgaccgcg gccacccgat aggaaacggt gatcgtgtcg ccgatgaaaa
    10621 cgggcttgag gaagcgcacc ttgtcgtagc ccgccgagac cggcgtgaag ggcgaattcg
    10681 ccgtcgtgta gcgcgagctc atcatcgacg ccgtgttcga ggagagtgcc agcacgagga
    10741 tgccgtgcgc gatcctccgg ccgaagaccg tcgtcttcat gtactcctcg ttgacatgca
    10801 ccttgtcgaa atccccgctg atgcctgaga acagatagaa atcggtctcg cccagggtct
    10861 tcgaaaaacg cacctcggcg ccgatctcgg ccggaatgac ggtcatggcg tgatctcctc
    10921 gtggctttct ggtcgaaggg cggccgcgaa ggccgacgtg atggcgggct tcgtcttgcc
    10981 ggcgcgcccc gcgtcgccga tgacgtcgat ccgggggccg gcgccgatgg cggcccggat
    11041 ggcggcgcca ctcggctgcc gggcccgatg atagagccag tcgcgcagcc ccggggcagc
    11101 gaagaggcgg cgggcgaggg cggttcggcc gaagccccgc tccaggagga agcccacgag
    11161 cggcccgagc ccgaagcggt aatgtgcccc gctcgcgatc accacccggt cgaagccacg
    11221 cagccgttcc ggcaccgcca gcacatccac ccccagctcg agccgcacgc cttgtcgcct
    11281 gagcgccgcc tcgagcccgg cgacataagc cccgatcggc cccttcgccg cctcgacgtc
    11341 ctggaaggtc ggcgccaatg ccgcctgcag caaggccccg ccgcactcgg catcacgctc
    11401 gaagaccgtc acctcgttgg cgcccgccac cagcccggca aagctgaggc cggccggccc
    11461 ggcaccgacg acggcgatcc gctcgccgcg gatcggcgag acctcgggca gctccgcctc
    11521 gcgccctgcg cgcgcattga ccaagcagcc gagcgctgcg ccgccgcgca tctcgttgac
    11581 gcaggtgttg caggcgatgc agcgcctgac aggctcgccc tgctccgcct tcgccaccca
    11641 gtccggatcg gccaggagcg ggcggccgag ggcgacgaag tcgcaggcgc cgtcctcgac
    11701 cgcggcgatg gccaccgccg gatcgccgag ccggccgacg gcgatcaccg gcacatccac
    11761 ctcccgcctg atcgccctgg caaaatccag gaagggtgcg tcgggctcgg ccatgggcgg
    11821 tgtcatgcgc atcgccgagg gcttcgagcg gtagtgcccg gccgtcacat ggatggcgtc
    11881 cgccccggcc tcggccgccc agagcgcgat ttggcgcccc tcctcgaagg tcagcccgcc
    11941 ctcgaagaaa tcgtcgacgg tgagccggta gatcatcgga aaatccggca gctccgcctt
    12001 gatccgccgg aggatgtcga gcccgaaccg ggcgcggttc tcgaggctgc cgccatagcg
    12061 atcctggcgc aggttctcgg ccggggtgtg gaactgcgag atcaggtagc cgtgggcggc
    12121 atggatctcg accatgtcga agccggcggc ctcggcgcgc acggcggccg ccacataggc
    12181 ccgtgtcgtc tgctcgatcc gctccgccgt catcgcctcg ggcacgatcg tggcaaaggt
    12241 gatctcgaag acgtcgtgcg gcacggcgga gggggctatc ggcacctcgc cgcagatgtc
    12301 atctcgcgtg tggcccccgc catggccgag ctggatggtg gtcctggccc cggcctcgcg
    12361 cagcgccgtc gtcagccgcc tgagccccgg caggaaccgg tcgtcataaa tcccgagctc
    12421 gcgccttcga tgccggcccg cgggctccgg tgccgccatc tcgacggtga cgaggccgac
    12481 cccgcccgtc gcccgggcga gataataggc gagcgtcgcc tccgtcaccc cgccggcctc
    12541 gtcggcgagg cgcgtcgtca tcgacggcag cacgatccgg tttttgatct ccacccggcc
    12601 aatcgaggcc ggggtgaaca actgcttcat gatcgggtca cgatgcggcg agcgccggtg
    12661 ccgggtggcc cgcctggcgc ccggccgtgc cggacggcac caccttgccg gtctcgacgt
    12721 cgatcaggtg ggcgcgcgcc atgtcgacgg tgagcggcac gcgatcgcct tggtcgcgcc
    12781 tgagataggg atcgcaggtg gcggcaacct cgacgccggc cacctggaag aaggcgaggc
    12841 cctcgttgcc catgggctcg accacgtcga tcgcggcctc gaacgtcgcg gcgtccggtc
    12901 cttcgaagcg gccgacatgc agcgtctccg gccgtacgcc gagctccatc ggccgatccc
    12961 gccatgcagc ataggcggca tcgcgcgccg ccggcagggg cagcaggatg ccgccctcga
    13021 tggcgaaggc gaggccattg ccctccgcca ccacccggcc cggcaggaag ttcatctgcg
    13081 gtgccccgat gaagccggcg acgaagcggt cgaccggctg ctcgtagacc tccatggggg
    13141 cacccacctg cacgatctgc ccgcccctca tgatgacgat gcgatcggcc agggtcatcg
    13201 cctcgatctg gtcgtgggtg acgtagatga ccgtcgtctc gagccgcttg tgcaggcgct
    13261 tgatctcgac ccgcatctgt gcccggaggc tggcatcgag attggagagc ggctcgtcga
    13321 agaggaacgc cttgggctgg cggacgatgg cccggcccat ggcgacgcgc tggcgctggc
    13381 cgccggagag ctcacgaggc ttgcggcgca ggagctccgt gatgttcagc agggccgccg
    13441 cgtcctgcac ccgacggcgg atctcgtcgc ggcccatgcc cttgaggcgc aggccgaacg
    13501 acatgttgtc gaagaccgtc atgtgcgggt agagcgcgta ggactggaag accatggcga
    13561 tgtcacggtt cttcggcgtc aggttgttga ccacgtactg gtcgaacttg atcttgccgc
    13621 ccgacaccgg ctcgagcccg gcgaccatgc gcagcgtcgt cgacttgccg cagcccgagg
    13681 gtccgacgag cacgacgaac tcgccatcgg cgatcgcgag atcgatgccg gcgacggcaa
    13741 ccacgtcgcc gaaacgcttc tcgaccttct ccagcctgat cgtcgccatc tgtctccccg
    13801 aagttgccgg ctttggccgc ggcggccttc aacccttcac accgccggcg ccccagccgg
    13861 cgatcagata gcgctgcacc aggacgaaga agatcagcgc cggcagggtg atgaccacgc
    13921 ccgccgccat catcatgccc cagtcggtga ccgtcgagac atagatgtcg ttgatgccga
    13981 ccggcagcgt cttcaggttg tcggagctga tcagcacgcg ggcgaaaaga tagtcgttcc
    14041 aggcgacgat gaaggtgaag atcgtcaccg cgatcacccc gggcagggcg agcggcacga
    14101 tcacccggac caccgcctgc ggccgcgagg cgccgtcggt catcgccgcc tcctcgaggt
    14161 cgagcgggat cgactggaag aaggagcgga gcagccacat cgagaacggc agcgagaagg
    14221 tggcgtagga cagcacgagg ccgaagagcg agttcacgag gccgagctgc cgcatcagga
    14281 tgtagaacgg caccacgatc atgatcggcg cgaacatgta ggtgaagagc atggtggtcg
    14341 agatcagctc gcgaccgcgg aagcgaaagc gcgtgatgcc gtaggcggcg agcgtgccga
    14401 ccgccacggt cagcagcgtg gcgaggccgg cgacgatgat gctgttgcgg aaataggtgg
    14461 cgaagatggt gctggtgaaa agccggcgat aggcgtcgat ggtcagctcg ctcgggatga
    14521 aggtcggcac cgccgtcagg atctcgcggt ccggcttgat cgaggtcagg accatccagg
    14581 caaggggaaa ggcgcagatc accagcagga gggcggcgag cccgtagacc cagaggcgaa
    14641 agagggcggc gcgcgggttc atggccggct catggttcgt tggcccgctt gaacaggcgg
    14701 aaatagagca gcgcgaaggc cacgaggatg aggaagatca cgaccgagag ggcggcgccc
    14761 atgccgacct gcagctcctg gaaggtcttg acgtaggtgt agatgggcag cgtgcgggtg
    14821 aagtagccga cgccggtgcc gctggcgaag agccagatga tgtcgaactt ggtgaacatg
    14881 aagagaaagc gcagcagcac caggacgaag agcacgctgc ggatctgcgg cagggtgatg
    14941 tgccagaagc ggcggagcgg cccggccccg tccaccttcg ccgcaccgta gagctcgggg
    15001 tcgatggtct gcaggcgggc cagcgtgccg atcacgatga acgggaagta ggtccaggtg
    15061 ctgatcagga tgagggagag catgatgctc tcggggccca gcatcagccc cggctccagc
    15121 ccgacgaggc gaagcgcgta gggcaggacc ccgtaattgt cgttgaggat ccagcgcatc
    15181 aggatcacca cgacgatcgt cggcaccatg tagggaaaaa gggcgcagcc gcggacgaag
    15241 ccgcggccga tgaaggtctg gttgagcagg agggcggcgc ccacgccgaa gacgagctgc
    15301 agggcgaccg tgccgaaggc gtagacggtg ccgagccaga ggccgcgcca gaagccgtcg
    15361 aaatcggtga ggaagtagcg gaaattgtcg aggccgacga aggtctcgat tggcaggtag
    15421 gcgtgctttg agtgcacgct gagccagaag gagtagataa ccgggtagag gatcagcccg
    15481 aggacgagca gcatggccgg ggcgaccagc acatagcccc accagtgctc cgggatgcgg
    15541 cggccgcgaa gccagacctc cgcttccccg ttatcggccg ccagtgtgct gccgtcttgc
    15601 gtggcgactg ccatcccctg cctttcggat cgggccgcgc cctggggcgc ggcccgcgac
    15661 gaagataccg tcagcctacc acttgcgata gccgagctgg gttatcagct cctcggcccg
    15721 gccctgcgcc cgctcggccg cctccatggg cgtgcggatg ccctggacga cgtccgtcac
    15781 catgtcggcg agaatgccgg agccggcgat ctcggcggtg aagggcagct tcagatcctc
    15841 ccactgggtc atcaggagcg gccgcgggtc gtcctcggcg atgatcttga gctgggcctg
    15901 gacccagtcc tcccacttct cgaacgccgg attggcgaga taggcgggat cggcggctat
    15961 cgacttcctg atcggcagga gatgcgtcgg caccgactcg acgtatttca ggtagttcgc
    16021 cggctcgtag aagaactcga ggaactcgac cgcgacatcc gcgttgtccg catccttgaa
    16081 gacgatccag gactcgttgt cgaactgggt gacgaacttg ccgggctcgc cgctcgggcc
    16141 ggccggcttg gaatcgaaga cggcgatatc gccggcggcg acggcatcgg gcgcgtactg
    16201 ctcgaaatag ccggtgccgc ggccccagcc gaggatcgag gcaacctggc cggtggccag
    16261 gttggcgaag gtgtcgagat agctctggct cagccagtcc ggcgacaggc agcagtcgtt
    16321 gagctccttc cagaactcga gcgtctcgat aacctggggc tcggtgaagg tcggccggcc
    16381 gacttcgtcg aagaggcgcc cgccattcga gccgacccac atatagagat cctcgttgat
    16441 gaagaacgag gggccggcga ggccgaggcc gtagcggtcc atgcggccgt cgccgtcgct
    16501 gtcgatcgtg agcgccttga gctgctcgag ccactcgtcc cacgtggcgg gggccgtgtc
    16561 gggatcgagg ccggcctcgc ggtagaaatc cttgcggatg atgatcgtgt cggcgccgac
    16621 ggcgtgcgag atgccgtaat tgttgccgtc gtcataggtg tgaatgaagt tgatgacatc
    16681 gaagatgtcg tcgcgtccga tcgagtcgaa gacgtcgttg agcggcatca gcatgccctt
    16741 ggcagccagc gagcgctcga cataggtctg ggcctgagtg agggtcggcg gtgcgccggc
    16801 ggccagcgcc gcctgcatct tcaggtcgag atcgccccag cccaccgcct cgtgctcgat
    16861 gcgcacatca ggatgggtct cgatgaagtc ggcgatgatc tcgtccatca cggccttggt
    16921 cgccgggttg ggctcggtgt gccagacctg gatgacccgc tcctgggcgg cgagggcggt
    16981 cggcaggagg agggcgaggg tggcaccggc gaggagggtg cgataaccgt atcgccgcga
    17041 ttc
//
LOCUS       DAOTFI010000102        17006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4059_length_17006_cov_6.802195, whole genome shotgun sequence.
ACCESSION   DAOTFI010000102 DAOTFI010000000
VERSION     DAOTFI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4059_length_17006_cov_6.802195"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="P5558_11875"
     CDS             complement(<1..197)
                     /locus_tag="P5558_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25055.1"
                     /translation="MGSAGQTLVIIAAGIDLSVAAVITFAAILTPIVSAGWDPTGLVG
                     ILVVLAITTAIGAVNGVGVVLL"
     assembly_gap    276..285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <286..882
                     /locus_tag="P5558_11880"
     CDS             <286..882
                     /locus_tag="P5558_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25056.1"
                     /translation="RVHPLIITLATATILQGSLLLIAGGSAVSTTNPAVVWLGNERLW
                     GFPVTILTWLVVAAATLVVLHRTVLGGWLYAIGTNPRASRLAGVNAPLTLITVYAISG
                     ASAGLAGLLLLGMNRQGYVGIGEPYLLGTIAAVVVGGTSILGGRGSYLGTIAGAILLV
                     TLTALITVVNASPGLRMILFGGLVLAMLLIAGRVQETR"
     gene            1014..1154
                     /locus_tag="P5558_11885"
     CDS             1014..1154
                     /locus_tag="P5558_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25057.1"
                     /translation="MFAHVESIASVVLWAGADQDVIIVNGATSLDSIGFVEGTLNQEV
                     VA"
     gene            complement(1164..3536)
                     /locus_tag="P5558_11890"
     CDS             complement(1164..3536)
                     /locus_tag="P5558_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRY25058.1"
                     /translation="MSVLRPVEPRREAPGRPGIAPTWTSSAKDIVGCALGPSRVWFTM
                     GYGIVNEVYYPRVDLPQIRDLGFIVADGEGFWVEVKRLGNYTLRLVEPGVPAVEVRHR
                     HERFELHLRVTPDPIRDVLLVELCLDGDAALRPYVLLAPHLGDSGWNNEAAVMELGGR
                     RVLTAEQGPFGLALAAVDERQQDAFGEASAGHVGVSDGWQDFDRHGAMRWQHSVAGPG
                     NVALTGALPRFAVLGLGFGSTAQAAATLAVSSLQGPFERHLARQRDDWRAWHGKVRGQ
                     GMGGLLEPASIEAHVGLSAMVLRTLRDQTYPGTMVASLSVPWGNTRDDLAGYHLVWPR
                     DLVACSTALLALGAVDEARDSLRYLIATQAADGYWAQNQWLGGTPHWTGIQLDETAAP
                     VLLAAALAERDALDGIEVGDMVRRALRFIARTGPGSDQDRWEENAGINAFTLAFAIAA
                     LVEGARFVDAADAALATALADFWNAGIERWTAVADTPLARRLGVAGYYTRMAPIEVMA
                     ESRPLQHLLVLKNRESSSAGVAADEEVSTDFLQLVRFGLRSPDDAHVAASLRVIDALL
                     RADTPNGPVWRRYNGDGYGEHDDGRAFDGTGRGRGWPLLTGERGHVELVAGRDPLPFL
                     DAMAAMAGPSGLLPEQVWDAEPLADAGLLTGRPTGSAMPLGWAHAEYIKLALSRQSGA
                     PVDRPAAVWRRYGGKVPKADRHFWLPHAPIAGLPGQATLVIAFPGEGQVHWGVDDWQA
                     VVDEPTKELGLGLHGIELSPDRLGPASRIVFTWRTADGAWSGHDVVVTRG"
     gene            complement(3605..4402)
                     /locus_tag="P5558_11895"
     CDS             complement(3605..4402)
                     /locus_tag="P5558_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY25059.1"
                     /translation="MGRWLVRGLCLGLLAFVVFGPLANMVLWAFTERWYFPHALPIEY
                     GFSFWGRVFAPRGGAIESLVNSAVIAVGTVAMSLALAVPAGYALARLRLPFRSLILLA
                     FLLPQAFPNLPVYINIARIFYGFGLNGTYLGVVLVHTSHGLVYAVWIATAAFASVEHE
                     LEEAARNIGAGAMHTFWTITLPLAAPGLMASAIFVFLESLDEFTGTYFVGAPDVQTLP
                     LLLYSASAGGNYQIASITALILLVPSIAFMLVVERFLRADVLAKVGR"
     gene            complement(4402..5235)
                     /locus_tag="P5558_11900"
     CDS             complement(4402..5235)
                     /locus_tag="P5558_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY25060.1"
                     /translation="MKASPFLGPLLVLPALGVILLLFVWPLLYSLVGAFVTGEGWGLG
                     NFAKTVELYRSDILFTFLIVGLSTLLIAVFAVAIGGYLVLGSNPRAQAILRWLYRWPL
                     FIPFIVAGQLMRTFLAKNGLLNGMLVEAGLLTPLQTLSFLDWRGIVIAFVWKQTPFVA
                     LLVAGAMAGLDRSMFEAARNLGASRLRILVEIVVPQVMQTLLVGLILSFVIMLSVLSV
                     PLMINAQTPTMITANMAFRINAYGDYGVANALGVISYLMAGAVAWIYLRYAVRERMEQ
                     R"
     gene            complement(5285..6433)
                     /locus_tag="P5558_11905"
     CDS             complement(5285..6433)
                     /locus_tag="P5558_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25061.1"
                     /translation="MRQSRAMIASLIGGTMLATTSLAMAETLTVVTAGDQNMVDYINE
                     FLAPRFEAANPGNTVRALGTGPGDAGSQMIYERLAAQKAAGATDVDVDVAVVHQKMAG
                     QMVEEGLLESYRDKIGTGDLVTRDTAKMALGTDVDGYVMPMFHSQTAIAYNPALVPEP
                     PQTYEELVEWVAENPGQFGYNGIKGGMSGVSFVMGWMYAFTDQGEQLMNGPFDESTEA
                     AWSDALAKLKAFNENVTFSPGNAGTLDMLNRGEIAMGPVWVDMFYTWQADGRLNPDLK
                     LSLIGPGMPGQPMYYVVPEGAANAELAAKFIELATSPAVQAEGIVERFNWYPGIDAQH
                     VQAELDDATWQRLFADVGPEDLAEKGKPFPIGPYFDAILEAYERQVTN"
     gene            complement(6435..7565)
                     /locus_tag="P5558_11910"
     CDS             complement(6435..7565)
                     /locus_tag="P5558_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25062.1"
                     /translation="MPSEAFLDIDRVAVRYGSTPVLHDLSLAVGRGELVALLGSSGCG
                     KTTLLRTIAGFLSPTSGRLRIGERDITRLAPDRRDMAMVFQSYALWPHMSVAQNIGYG
                     LKLRRLDRAAIRAKVEDLLAMLGLAGLGERNVTALSGGQRQRVALGRALAVDPEILLL
                     DEPLSNLDARIRQTVRHEIRALQRRLGITAIHVTHDREEAMVMADRIVILDAGRIAQA
                     GPPERIYNQPASPFVADFMGADNSVTLEADLDGASVVIAAGADHAAARLARDLLPTAN
                     TLPGQGTFSLRAFFRGEAAALVAADEAVTGDETEALLLTGRVLAVSYPGGVWRHEVAI
                     GERHFLVDDATCHAPGDAVAVRLPARHLFLFPADGTTKPRGG"
     gene            7767..8756
                     /locus_tag="P5558_11915"
     CDS             7767..8756
                     /locus_tag="P5558_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRY25063.1"
                     /translation="MSDWLERSYMARLGVAKGREGRRHQLDRERQGAFHYVYGPYAEP
                     VLTIDPGDIVVAETEDAFGGAIRSERDLPSAVLNMPFVNPQCGPIRIEGAKKGDVVAV
                     RILDIEPRGPQPVGTTALIPEFGGLVATPVTAMLNRPLPERVKKMAVTREGVVFNERI
                     TLPYEPFIGTLGVSPEIEAISSLTPDYYGGNMDLPDMGPGAIAYFPVQHEGAFLYVGD
                     CHGTQGDGELCGVAVEIPSTTTLHVDLIKGWPIHWPRLENERFIMSIGSARPMEDAAR
                     IAYRDLVRWLVADYGFEESEAYFLLTQAGRVRLGNMVDPKYTLGASLLKAYLD"
     gene            8773..9534
                     /locus_tag="P5558_11920"
     CDS             8773..9534
                     /locus_tag="P5558_11920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY25064.1"
                     /translation="MDLGLKGLRAIVTGGTKGIGRRAADILADEGCHVSVCSRHADEV
                     KACVAALGKKGVKAHGQALDVADKAALESWVASAAAELGGIDIVVANVSALAGAGTEE
                     AWQQQFEVDVMHTVRTVNAAMPQLEASKAASVVIVSSVSGREVDFFAGSYGATKAALI
                     HYAHGLACQLAPKNIRVNSVSPGNVYFKDGIWNSIEKDNPELFKAALDLNPTGRMATA
                     EEVGRGIVFLASPASSFTSGTNLVIDGALTKGVQF"
     gene            9544..10812
                     /locus_tag="P5558_11925"
     CDS             9544..10812
                     /locus_tag="P5558_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY25065.1"
                     /translation="MNGAAARERFLDRAAALNERFHCFVTLTPDEVSTGVGPLAGVPM
                     AVKDCIPVRGVACTWGSALFRDRIAERDAPVVAQLRDAGVRIVGTANLHELAFGGTTQ
                     NPWYGNCRNAWNPERVPGGSSGGSAVAVALGLATLGVGTDTGCSVRLPASLNGLLGLR
                     PTHGAISNRDIMPVSPPHDTVGFIAADPETIRLAFTATCRHDPADPFSRSASTFARPG
                     RIRLGIASALLEETEPDIRDSFERAVEKLEGQGCSVQPVAEPALLEAALHIAPLAMAD
                     MAEFHRQRLAHDAARIGSAIHQRAARGLAMPATEYAGHLRWREANGVVINRLFETVDA
                     ILSPTVPVTAPTLAEAADAVGSTALLSRNCWAAPAAGIPALTVPCGRDRHGIPIGLQL
                     MGRAWSEHFLLDLAGLFLGPAGRARLPDQP"
     gene            10994..11161
                     /locus_tag="P5558_11930"
     CDS             10994..11161
                     /locus_tag="P5558_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25066.1"
                     /translation="MPLQAVPGGARPAIASPPCSYRVSHSSGETICPDLASARLLLSQ
                     KPGAKIFLQRG"
     gene            complement(11712..13304)
                     /locus_tag="P5558_11935"
     CDS             complement(11712..13304)
                     /locus_tag="P5558_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRY25067.1"
                     /translation="MRVMTIFALLALAMSIAVGGEAVSQTSTLSRAVQESDSRQPAIP
                     RPGQQAEAARKLADFEARTGRKPNILLFIVDDMGYGDPGAFGGGEAIGAATPNMDRLA
                     AEGLKLTSTYAQYTCTPTRAAILTGRLPVRTGLTRPILAGDNVTRNPWEGEVSLPGIL
                     SDHGYHTLLVGKWHVGEVEGMRPHDIGFDEFYGYYRAQKEYIQRHDGHRYPDLVLDAD
                     KRERYDAIGADDNLVRGLKDGTTETVMEVASVQDMAEGDGMLRDYTVARIAELARGAE
                     PFFLTHAFMKVHADNWPSAAFEGASSSKYPYKDAVVEVDSHIGDIMQALEDAGELENT
                     FVFVTSDNGPQMDGWPDSGYTPLRGAKGTTWEGGVRVPGIAYWQGMIEPGRVSDGLFD
                     LMDLFNTSVALAGAEDAIPTDRYIDGIDQTSFLLAGDGESRRENVYFWLGSQFTAMRM
                     REYKMHTKVVEPAEKFLWIDMATLQDTALAPWLFNLYIDPKEEYTVGHRLNAWQASLG
                     NEMKAHALTFRKFPPKDIGLGG"
     gene            complement(13292..14446)
                     /locus_tag="P5558_11940"
     CDS             complement(13292..14446)
                     /locus_tag="P5558_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY25068.1"
                     /translation="MIEPLRALKMSALVGAALITTAEPVRAQDQAKERPNILVIMPDD
                     VGLWNISAYNRGSRGYRTPSIDRLAEEGALFSDMYAQPSCTPGRASFITGMYPIRSGL
                     TTVGMVGAKQGMRAEVPTLAEVLKQQGYATGQFGKNHLGDRNEHLPTVHGFDEFYGFL
                     YHLNVLQEPLDNDYPKSDAFRAKFEPRGIIHSFATDTYDASVPDDRFGEVGKQRVEDT
                     GLPDEQRFETLDAELNGKAFDFMERAVAADEPFFVWLNPSRMHVFTFTPDAYKERARE
                     HTSYDDPHGAGMIQHDDDIGAVLAKLDKLGVGDDTIVVYTTDNGPEHSTHPHGGITRI
                     RRPTEPSAATGGKRGARQRWRGTEGHHRPDREAAAACQLKSRERRKPCAS"
     gene            complement(14500..16860)
                     /locus_tag="P5558_11945"
     CDS             complement(14500..16860)
                     /locus_tag="P5558_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRY25069.1"
                     /translation="MTARLVILAALASLLAAGSAGTQEKDDGSVLPFPPEPMAGEAKQ
                     LLQDSTMQWPEPARHLAENAPNILIVMLDDVGFGISEVFGGEVRTPAFARLAAEGIAY
                     NAFNTASICSPSRAALLTGRNHTRVGSGTIAERAVAFDGYTGVIPKTAATIPEILRQY
                     GYHTAAFGKWHNTPAIETTSIGPKDHWPNAYGFEHFYGFLGGETSQWEPRLTRNYDHV
                     EPPADDPTYHLTEDLASRALAWLDDYRAFDPARPYFMYWAPGGVHGPHHIFSEWADKY
                     DGAFDDGWDAYRERTWRRQLDMGVIPAGTELTPRDATMASWDSIPADQFDFQTRLMEV
                     FAGYVEHTDTQVGKLIDGLEARGLRDNTLIFYIFGDNGSSAEGQEGSISELLAQNNIQ
                     TTTDQQLEALEKLGGTAVLGSPKTDNIYHAGWAWAGGTPFKGTKLLASYFGGTRNPLV
                     ISWPGHISHDRRMRPQFHHLVDVAPTIYDILQITPPKVVNGYDQLPLDGISMAYTFGD
                     AGAAGQKSEQFFDNNGSRGIYMDGWFAGTFGPLIPWDTAGSAKGLADWDSAKDQWELY
                     DLGRDFSQARDLAAAEPKRLLRMKKRFLEVARENQDFPIGAGLWLRIHPEDRVKTAYD
                     HWTFTGSTERMPEFTAPGVGRQSTTVTIDAEFEDGANGVLYAVGGAGGGLSVYMRDGR
                     LVYEYNMLLIENYAVTTDPIPGGRHEIVIDTEIEKPGGAAQVVITVDGKVAGRTTVKR
                     TVPAAFTATETFDVGRDLGSPVSTAYADERPFAFDGSIRGMAVTMK"
BASE COUNT         2620 a         6043 c         5651 g         2682 t
ORIGIN      
        1 agcagcacga cgccgacgcc gttgacggca ccgatggcgg tggtgatggc gagcacgacg
       61 aggatgccga cgaggcccgt ggggtcccag ccggccgaga cgatgggggt gaggatggcg
      121 gcgaaggtga tgacggcggc gaccgagagg tcgatgccgg cggcgatgat gaccagcgtc
      181 tggccggcgg accccacggg gctcctcggc atcctcttcg tgctcgccat caccaccgcc
      241 atcggcgccg tcaacggcgt cggggtcgtg ctgctnnnnn nnnnncgcgt ccacccgctc
      301 atcatcacgc tggccaccgc cacgatcctg cagggctcgc tcctgctcat cgccggcggc
      361 agcgccgtct cgaccaccaa ccccgccgtc gtctggctcg gcaacgagcg cctctggggc
      421 tttcccgtca ccatcctcac ctggctggtc gtcgccgccg ccacgctcgt cgtcctgcac
      481 cgcaccgtgc tcggcggctg gctctacgcc atcggcacca atccccgcgc cagccgcctc
      541 gccggggtga atgctcccct caccctgatc accgtctacg ccatctccgg cgcgtcggcc
      601 gggctcgccg gactcctgct gctcggcatg aaccgccagg gctatgtcgg catcggcgag
      661 ccctatctgc tcggcaccat cgccgccgtc gtggtcggcg gcacctccat cctcggcggc
      721 cgcggcagct atctcggcac catcgccggc gccatcctgc tggtcacgct caccgccctc
      781 atcaccgtgg tcaacgcctc gccgggcctg cgcatgatcc tcttcggcgg cctcgtcctc
      841 gccatgctgc tgatcgcggg acgcgtgcag gagacgaggt agttattcag tcccggagtg
      901 tggtggctcg gacggcatgc gtcgttgtgg cgcttgcttg cagtagctgg cccatcgttc
      961 ttccttggcg tgaccgcagc aacttgcacc atcacacgcc gggacttaac gaggtgttcg
     1021 ctcacgtcga atccatcgcc tcggtcgtcc tctgggccgg tgccgaccag gacgtgatca
     1081 tcgtcaacgg tgcgaccagc ctcgacagca tcggcttcgt cgagggcacg ctgaaccagg
     1141 aagtcgtcgc ctgagcccgg gactcagccg cgtgtcacca cgacatcgtg gccgctccat
     1201 gccccgtccg ccgtccgcca ggtgaagacg atgcgcgacg ccgggccgag ccggtccggt
     1261 gagagctcga tgccgtgcag cccgaggccg agctccttcg tcggctcgtc gaccacggcc
     1321 tgccagtcgt cgacgcccca gtggacctgc ccctcgcccg ggaacgcgat gacgagcgtc
     1381 gcctgcccgg gcaggccggc gatcggcgcg tgcggcagcc agaagtgccg gtcggccttc
     1441 ggcaccttgc cgccatagcg ccgccagacc gccgccgggc gatcgacagg cgcacccgac
     1501 tggcgcgaga gagcgagctt gatgtactcg gcatgcgccc agcccagcgg catggccgac
     1561 ccggtgggcc ggccggtgag caggccggca tcggccagcg gctcggcatc ccagacctgc
     1621 tccggcagca gccccgacgg accggccatc gccgccatgg cgtcgaggaa gggcagcgga
     1681 tcgcggccgg cgaccagctc gacatgcccg cgctcgccgg tcaggagcgg ccagccgcgg
     1741 ccgcggccgg tgccgtcgaa ggcgcggccg tcatcgtgct cgccatagcc gtcgccattg
     1801 tatcgccgcc agacgggccc gttcggcgtg tcggccctga gcagcgcgtc gatcacccgc
     1861 aggctggccg cgacatgcgc atcgtcgggc gagcggaggc cgaagcgcac gagctgcagg
     1921 aaatcggtgc tgacctcctc gtcggccgcc accccggcgc tgctcgattc ccggttcttc
     1981 agcaccagca aatgttgcag cggccggctt tccgccatca cctcgatcgg cgccatgcgc
     2041 gtgtagtagc ccgcgacacc cagccgccgt gccaaaggcg tgtctgccac cgccgtccag
     2101 cgctcgatcc cggcgttcca gaaatcggca agggccgtgg cgagcgccgc atcggctgca
     2161 tcgacaaaac gcgcaccctc gaccagggcc gcgatcgcga aggccagggt gaaggcgttg
     2221 atgcccgcat tctcctccca gcgatcctgg tcgctccccg gccccgtccg ggcgatgaag
     2281 cgcagcgccc ggcgcaccat gtcgcccacc tcgatgccgt ccagagcgtc ccgctcggcc
     2341 agcgctgccg cgagcagcac cggggccgcc gtctcgtcga gctggatgcc ggtccagtgc
     2401 ggcgtgccgc cgagccactg gttctgcgcc cagtagccgt ccgccgcctg ggtggcgatc
     2461 aggtagcgca ggctgtcgcg cgcctcgtcg accgcaccca gcgccagcag ggccgtggag
     2521 caggcgacca gatcgcgcgg ccagacgaga tgatagccgg ccaggtcgtc gcgggtgttg
     2581 ccccagggca cgctcaggct cgccaccatg gtgccgggat aggtctggtc gcgaagcgtg
     2641 cgcagcacca tggcggaaag cccgacatgc gcctcgatcg atgccggctc caggagcccg
     2701 cccatgccct ggccccgcac ctttccgtgc catgcccgcc agtcgtcgcg ctgccgggcc
     2761 aggtgccgct cgaacggacc ttgcaggctc gagacggcga gcgtcgccgc cgcctgtgcg
     2821 gtgctgccga agccgaggcc aagcacggcg aagcgcggca gggcacccgt gagcgccacg
     2881 ttgccggggc cggccacgct gtgctgccag cgcatggcgc catggcggtc gaaatcctgc
     2941 cagccgtcgc tcacgcccac atggccggcg ctggcctcgc cgaacgcatc ctgctgccgc
     3001 tcatcgaccg cggccagcgc caggccgaac ggcccctgct ccgccgtcag cacccggcgc
     3061 ccgccgagct ccatgaccgc tgcctcgttg ttccagccgg aatcacccag atgcggcgcc
     3121 agcagcacgt aaggccggag cgccgcgtca ccgtcgaggc agagctcgac gagcagcacg
     3181 tcgcggatgg gatccggcgt gacccgcaaa tgcagctcga agcgctcgtg ccggtgccga
     3241 acctcgaccg ccggcacgcc gggctcgacc agccgcagcg tgtagtttcc gagccgcttc
     3301 acctcgaccc agaagccctc gccgtcggcg acgatgaacc ccagatcgcg gatctgcggc
     3361 aggtccaccc tcgggtagta gacctcgttg acgatcccgt agcccatggt gaaccagacc
     3421 cgggacgggc ccagcgcgca gccgacgatg tccttcgcac tgctcgtcca ggtcggcgcg
     3481 atccccggcc gacccggtgc ctcccgacgc ggttccacgg gccgcagcac gctcatgacc
     3541 ggctcctccc ggcctccatt ggccgccaac atcgaaggcc tgtccagcgc aatcagcgcc
     3601 ggctctatcg ccccaccttc gccagcacgt ccgccctgag gaaccgctcg accaccagca
     3661 tgaaggcgat cgacggcacc agcaggatga gcgccgtgat cgaggcgatc tggtagttgc
     3721 cgccggcgct ggcgctgtag agcagcagcg gcagggtctg gacgtccggg gcgccgacga
     3781 aataggtacc ggtgaactcg tcgagcgatt cgaggaagac gaagatggcg ctggccataa
     3841 ggccgggtgc ggcgagcggc agggtgatcg tccagaaggt gtgcatggcc cccgccccga
     3901 tattgcgcgc cgcctcctcg agctcgtgct cgaccgaggc gaaggccgcc gtggcgatcc
     3961 agacggcata gacgaggccg tggctggtgt ggacgaggac gacgccgaga taggtgccgt
     4021 tcaggccgaa gccgtagaag atccgggcga tgttgatgta gacgggcagg ttcgggaagg
     4081 cctgcggcaa aaggaaggcc agcaggatca ggctgcggaa cggcagccgc aggcgagcca
     4141 gggcgtagcc cgccggcact gccagggcca gcgacatggc gaccgtgccg acggcgatga
     4201 cggcgctgtt gacgagcgat tcgatcgcgc cgccacgcgg cgcgaagacg cgcccccaga
     4261 agctgaagcc gtactcgatc ggcaaagcgt gcgggaagta ccagcgctcg gtgaacgccc
     4321 agagcaccat gttggcgagc ggcccgaaga cgacgaaggc gaggaggccg aggcacagcc
     4381 cccggaccag ccagcgcccc atcagcgctg ctccatccgc tcgcgcacgg cgtagcgcag
     4441 atagatccag gcgacggccc cggccatcag ataggagatc acgccgagcg cgttggcgac
     4501 cccgtaatcg ccataggcgt tgatccgaaa ggccatgttg gcggtgatca tggtgggtgt
     4561 ctgggcattg atcatcagcg gcaccgacag caccgagagc atgatgacga agctcaggat
     4621 gagcccgacc aggagcgtct gcatgacctg cggcacgacg atctcgacca ggatgcgcaa
     4681 gcgcgacgca ccgaggttgc gcgccgcctc gaacatcgac cggtccagcc cggccatggc
     4741 cccggcgacc agcagcgcca cgaacggcgt ctgcttccag acgaaggcga tgacgatgcc
     4801 gcgccagtcg agaaagctca gggtctgcaa gggtgtcagc agcccggcct cgaccagcat
     4861 gccgttcagg aggccgttct tggcgaggaa cgtgcgcatg agctggcccg ccacgatgaa
     4921 cgggatgaag agcggccagc gatagagcca gcgcaggatg gcctgcgccc tggggttgct
     4981 gccgagcacc aggtagccgc cgatggcgac cgcgaagacg gcgatcagca gcgtcgagag
     5041 cccgacgatg aggaaggtga agaggatgtc gctgcggtag agctcgaccg tcttggcgaa
     5101 attgccgagc ccccagccct cgccagtcac gaaggcaccg accagcgaat agaggagcgg
     5161 ccagacgaag agcagcagga tgacgccgag cgccggcagc accaggagtg gccccaggaa
     5221 cgggctcgcc ttcatcgcag cgggagggcg ggagcgccgc ctcggacgct cccgccggcc
     5281 tcggtcagtt cgtcacctgg cgctcgtagg cttccaggat ggcgtcgaaa taagggccga
     5341 tcgggaaggg cttgcccttc tcggccagat cctccggccc gacatcggcg aagagccgct
     5401 gccaggtggc gtcgtccagc tccgcctgga cgtgctgcgc gtcgatcccg ggataccagt
     5461 tgaagcgctc gacgatgccc tcggcctgga ccgccgggct ggtggcgagc tcgatgaact
     5521 tcgccgcgag ctcggcgttg gcggccccct cgggcacgac atagtacatc ggctggccgg
     5581 gcatgccggg gccgatcagc gatagcttga ggtccggatt cagccggccg tccgcctgcc
     5641 acgtgtagaa catgtcgacc cagacgggac ccatcgcgat ctcgccgcga ttgagcatgt
     5701 cgagcgtgcc ggcattgccc gggctgaagg tgacgttctc gttgaacgcc ttgagcttcg
     5761 ccagcgcgtc ggaccaggcg gcctccgtcg attcgtcgaa cgggccgttc atgagctgct
     5821 cgccctggtc ggtgaaggcg tacatccagc ccatgacgaa gctgacgccc gacatcccgc
     5881 ccttgatgcc gttatagccg aactgcccgg ggttctcggc gacccactcg accagctcct
     5941 cgtaggtctg cggcggctcc ggcaccagcg ccgggttgta ggcgatcgcc gtctggctgt
     6001 ggaacatcgg catgacatag ccgtcgacat ccgtgccgag cgccatcttc gccgtgtcgc
     6061 gggtcacgag atcgcccgtc ccgatcttgt cccggtagct ctcgagcagg ccttcctcca
     6121 ccatctggcc ggccatcttc tggtgcacca ccgccacatc cacatcgacg tccgtggccc
     6181 ccgccgcctt ctgtgcggcg agccgctcgt agatcatctg cgagccggca tcgcccggcc
     6241 ccgtgccaag ggcccgcacc gtgttgcccg gattggccgc ctcgaacctg ggtgcgagga
     6301 actcgttgat gtagtcgacc atgttctggt cgccggcggt caccacggtc agcgtctcgg
     6361 ccatcgccag cgacgttgtc gccagcatgg tgccgccgat caggcttgcg atcatcgctc
     6421 gtgactgtct cacgttaccc tcctctgggc ttggttgtgc cgtctgcggg aaaaagaaag
     6481 agatgccggg ccggcagccg cacggcgacc gcatcgcccg gcgcgtggca ggtggcatcg
     6541 tcgaccagga aatgccgctc gccaatggca acctcgtgac gccagacgcc gcccggatag
     6601 ctcacggcga gcaccctgcc ggtcagcagc aacgcttcgg tctcgtcgcc cgtcacggcc
     6661 tcgtccgccg cgacgagggc cgccgcctcg ccgcggaaga acgcgcgcag gctgaacgtc
     6721 ccctgccccg gcaaggtgtt ggcggtcggc agcaggtcgc gggcgagccg tgccgccgca
     6781 tggtcggcgc cggcggcgat caccacactg gcgccgtcca gatcggcctc gagcgtcacg
     6841 ctgttgtccg cccccatgaa gtcggcgacg aacgggctcg ccggctggtt gtagatccgc
     6901 tcgggcggcc ccgcctgggc gatccggccg gcatcgagaa tgacgatccg gtcggccatg
     6961 accatcgcct cctcgcggtc atgggtgacg tggatcgcgg tgatgccgag ccgccgctgc
     7021 agggcccgga tctcgtgccg caccgtctgg cggatgcgcg cgtcgagatt ggagagcggc
     7081 tcgtcgagca gcaggatctc cggatcgacg gcgagcgccc tgcccagcgc cacgcgctgc
     7141 cgctgcccgc cggaaagggc cgtgacgttg cgttcgccga gcccggccag cccgagcatg
     7201 gcgagaaggt cctcgacctt ggcccggatc gccgcgcgat cgagccgccg cagcttcagc
     7261 ccgtagccga tgttctgcgc cacgctcatg tgcggccaga gcgcatagga ctggaagacc
     7321 atggccatgt cgcgccggtc gggtgcgagc cgggtgatgt cgcgctcgcc gatccgcaac
     7381 cgcccgctcg tcgggctgag gaagccggca atcgtgcgca gcagcgtggt cttgccgcaa
     7441 cccgacgagc cgagcaatgc cacgagctcg ccccgaccca ccgccagcga caggtcgtgc
     7501 agcaccggcg tcgagccgta acggaccgcc acccggtcga tgtcgaggaa tgcttcgctg
     7561 ggcataacgc ttcgccgctg tcccggcggc gtcggccgca cgggcttctt cgttcgaccg
     7621 acgccccctg gatcggctcg agtcaacggc gtgacaatga cgcgccccgg cccgctcgcc
     7681 aagcccgaag atcggttccc ggctctggac gggagccgga agcggtgcca ccttccgcga
     7741 cggttcggca cgagggagga acgagcatga gcgactggct cgagcgaagc tatatggcac
     7801 ggctcggcgt ggccaagggt cgggagggtc ggcgacacca gctcgaccgt gagcggcagg
     7861 gcgccttcca ctatgtctac ggcccctatg ccgagccggt cctgaccatc gatccgggcg
     7921 acatcgtcgt ggccgaaacc gaggacgcct tcggcggcgc catccggagc gagcgcgacc
     7981 tgccgtccgc cgtgctcaac atgcccttcg tcaacccgca atgcggcccg atccgcatcg
     8041 agggcgcgaa gaagggcgac gtcgtcgcgg tccgcatcct cgacatcgag ccgcgcggcc
     8101 cgcaaccggt cggcaccacc gccctcatcc ccgagttcgg cggcctcgtc gccaccccgg
     8161 tcaccgccat gctcaaccgg cccctgcccg agcgggtcaa gaagatggcg gtgacccgcg
     8221 aaggtgtcgt cttcaacgag cgcatcaccc tgccttacga gcccttcatc ggcacgctgg
     8281 gcgtcagccc cgagatcgag gcgatcagct cgctcacgcc ggactactac ggcggcaaca
     8341 tggatctccc ggacatgggc cccggcgcca tcgcctattt cccggtgcag cacgagggcg
     8401 ccttcctcta tgtcggcgac tgccacggca cccagggcga cggcgagctc tgcggcgtcg
     8461 cggtcgagat cccgtccacg acgacgctgc atgtcgacct gatcaagggc tggccgatcc
     8521 attggccgag gctcgagaac gagcggttca tcatgtcgat cggcagtgcc cgccccatgg
     8581 aggacgcggc gcgcatcgcc tatcgcgacc tcgtccgctg gctggtcgcc gattacggct
     8641 tcgaggagag cgaggcctat ttcctcctga cccaggccgg ccgggtccgc ctcggcaaca
     8701 tggtcgaccc caagtacacg ctcggcgcct cactcctcaa agcctatctc gactgaacag
     8761 ggagcagaaa cgatggatct cggtctcaag ggtctcaggg cgatcgtcac cggcggcacc
     8821 aagggcatcg gtcgccgggc cgccgacatc ctcgccgacg agggctgcca tgtcagcgtc
     8881 tgctcgcgcc acgcggacga ggtgaaggcc tgcgtcgccg cgctcgggaa gaagggcgtg
     8941 aaggcccacg ggcaggcgct cgacgtcgcc gacaaggcgg ctctcgaaag ctgggtggcg
     9001 agcgctgcgg ccgagctcgg cggcatcgac atcgtcgtcg ccaatgtctc ggcgctggcc
     9061 ggcgccggca ccgaggaggc atggcagcag cagttcgagg tcgacgtcat gcacacggtg
     9121 cgcaccgtga acgccgccat gccgcagctc gaggcgtcga aggccgcctc ggttgtcatc
     9181 gtctccagcg tctccggccg cgaggtcgac ttcttcgccg gctcctacgg cgccaccaag
     9241 gccgccctca tccactatgc ccacggcctc gcctgccagc tcgcgcccaa gaacatccgg
     9301 gtgaactcgg tctcgcccgg caatgtctac ttcaaggacg gcatctggaa ctcgatcgag
     9361 aaagacaatc ccgagctctt caaggccgcc ctcgacctca acccgaccgg ccgcatggcg
     9421 accgccgagg aggtcggccg cggcatcgtc ttcctcgcca gtcccgcctc gagcttcact
     9481 tccggcacca acctcgtgat cgacggggcg ctcaccaagg gcgtgcagtt ctaggccgtc
     9541 ccgatgaacg gcgccgccgc acgagagcgc ttcctcgacc gtgcggcggc gctgaacgag
     9601 cgcttccact gcttcgtcac tctcaccccc gacgaggtat cgaccggtgt cgggccgctc
     9661 gccggcgtgc ctatggcggt caaggactgc atcccggtca ggggtgtcgc ctgcacctgg
     9721 gggagcgccc tcttcaggga ccgcatcgcc gaaagggacg cgcccgtcgt ggcgcaactg
     9781 cgcgacgcgg gtgttcgcat cgtcggcacc gccaacctgc acgagctcgc cttcggcggc
     9841 acgacgcaga acccctggta cggcaattgc cgcaacgcct ggaaccccga gcgcgtgccg
     9901 ggcggctcga gcggcgggtc cgccgtggcg gtggcgctcg gcctcgccac gctcggcgtc
     9961 ggcaccgaca cgggctgctc ggtccgcctg ccggcctcgc tcaacgggct cctcggcctg
    10021 cgcccgaccc atggcgccat ctccaaccgg gacatcatgc cggtcagccc gccgcacgac
    10081 acggtgggct tcatcgccgc cgaccccgag acgatccgcc tggccttcac cgcgacctgc
    10141 cgccacgatc cggccgaccc cttctcccgc tccgcttcga ccttcgcccg cccgggacgg
    10201 atcaggctcg gcatcgccag cgccctcctg gaagaaaccg agcccgacat ccgcgactcc
    10261 ttcgagcgcg cggtcgaaaa gctcgagggc cagggctgct cggtccagcc cgtcgccgag
    10321 ccggccctcc tcgaggccgc cctgcacatc gccccgctcg ccatggccga catggccgag
    10381 ttccatcgcc agcgcctcgc ccacgacgcc gcccgcatcg gcagcgccat ccaccagcgt
    10441 gccgcccgcg gcctcgccat gccggccacc gagtatgccg gccacctgcg ctggcgggag
    10501 gccaatggcg tcgtcatcaa ccgactcttc gagaccgtcg acgcgatcct ctcgcccacc
    10561 gtgccggtga ccgccccgac cctggcggaa gcggcggatg cggtcggcag caccgccctg
    10621 ctgagccgca attgctgggc agcgccggcg gccggcatcc ccgccctgac cgtgccgtgc
    10681 ggccgggacc gtcacggcat cccgatcggc ctgcagctga tgggcagggc ctggtcggag
    10741 cacttcctcc tcgacctcgc ggggctcttc ctcggcccgg ctggccgtgc ccggctgccg
    10801 gaccagccct gagtggcgtt tccgcgtccc cgagtgtggc aagcagacaa cagcggcgga
    10861 atcgccgccg ccgttagtgg catttgaacc atgggatgca catcatagca gccggaccag
    10921 cgtaaggctt tcgacgacca tggaacagat tggctcctcg accgacgagc aaccgcagca
    10981 gccgatgcag ggcatgccct tgcaagccgt gcccggtggg gcgaggccgg cgatcgcgtc
    11041 gccgccctgc agctatcggg tgagccattc ctcgggcgag accatctgcc ccgacctggc
    11101 ctccgcccgc ctgctcctca gccagaagcc gggtgccaag atcttcctgc agcgcggctg
    11161 actggccggc gtcgggcaac aggcgcagcg tccctttccc gggcggcacg gtccagctgt
    11221 tggcgctgtc cgcttcccgg ctcgccgtga tgaccggcga cgacaccggg tgccagccgc
    11281 cctcgaggtt gtcgccggcg aaggatcaga gctagcggac gagcctgccc gaccatccag
    11341 gggcccggcg gcgccagcac gacagcggcc gggccggcgc tccatttccc gctgccgagc
    11401 ttctcgtcgg tggcgcacgg cacggtgagc gtcggcccga cgccaattcc tcgagctgcg
    11461 gttgcttgcc gtcgatcttc acgtcgcccc agacgatggc gtccgcgagc gaggtctgct
    11521 gcggcatgag gctgttcagc gcttcacggg acagggtgca gccgcgacgt ctcgatgagc
    11581 cgagctgcga tcgccccggc tcgcccagga atttgcgcgg atcgggagcc gggcccggct
    11641 cggcgatcgc cccgggcagt tcgagaggct cgccgagagc cacccgatcc cctcgttccg
    11701 gcaaccacct gctacccgcc gaggccgata tccttaggcg ggaatttccg gaaggtcagg
    11761 gcatgggcct tcatctcgtt gcccagcgac gcctgccaag cattcaggcg atggccgacc
    11821 gtgtactcct ccttcgggtc gatgtagaga ttgaacagcc acggcgcgag cgcggtatcc
    11881 tgcagcgtgg ccatgtcgat ccaaaggaac ttctccgccg gctcgaccac cttggtgtgc
    11941 atcttgtact cgcgcatccg catggcggtg aactgcgagc cgagccagaa atacacgttc
    12001 tcccgccgcg actcgccgtc gccggccaga aggaacgagg tctggtcgat cccgtcgatg
    12061 tagcggtcgg tcgggatcgc gtcctcggca ccggccagcg ccaccgaggt attgaagagg
    12121 tccatgaggt cgaacagccc gtccgatacc cggcccggct cgatcatgcc ctgccagtag
    12181 gcgatgccgg gaacgcgcac gccaccttcc caggtcgtac ccttcgcgcc gcggagcggc
    12241 gtgtagccgc tgtccggcca gccatccatc tgcggcccgt tgtccgaggt aacgaagacg
    12301 aaggtattct ccagctcgcc cgcatcctcc agcgcctgca tgatgtcgcc gatatggctg
    12361 tcgacctcga cgacggcgtc tttgtaggga tacttggacg agctagcgcc ctcgaaggcc
    12421 gccgacggcc agttgtcggc gtgcaccttc atgaaggcgt gggtcaggaa gaagggctcg
    12481 gcgccccggg ccagctcggc gatgcgtgcc accgtgtagt cgcgcagcat gccgtcgccc
    12541 tcggccatgt cctggacgga ggcgacctcc atcaccgtct cggtggtccc gtccttcagc
    12601 ccgcggacga ggttgtcgtc ggcgccgatc gcgtcgtagc gctcgcgctt gtccgcatcg
    12661 agcaccaggt cgggatagcg gtgcccgtca tggcgctgga tgtactcctt ctgcgcccgg
    12721 tagtagccat agaactcgtc gaacccgatg tcgtgcggcc gcatgccttc gacctcgccg
    12781 acatgccact tgccgaccag gagcgtgtga tagccgtgat ccgacaggat gcccggcagc
    12841 gacacctcgc cctcccacgg gtttcgggtc acgttgtcgc cggcgaggat tgggcgggtc
    12901 aggccggtcc tgaccggcag gcggccggtc aggatcgcgg cccgcgtcgg cgtgcaggtg
    12961 tactgggcgt aggtcgaggt gagcttcagc ccttctgccg ccagccggtc catgttgggg
    13021 gtcgccgccc cgatcgcctc gccgccgccg aacgcacccg gatcgccgta gcccatgtcg
    13081 tcgacgatga agagcaggat gttcggcttc cgccccgtcc tggcctcgaa gtcggcgagc
    13141 tttcgcgccg cctcggcctg ctgcccgggc cgcgggatcg cgggttgccg gctgtccgac
    13201 tcctggaccg cccgtgacag ggtcgaggtc tgggacacgg cctcgccgcc cacggcgatc
    13261 gacatcgcca gggcgagcaa ggcgaaaatg gtcatgacgc gcatggcttc ctccgttctc
    13321 tcgacttcag ctggcaggca gcagcagctt cacgatcggg ccgatggtgc ccttcggtcc
    13381 cgcgccatcg ttgtcgagca ccccgcttcc cacccgtcgc tgccgaaggc tcagttggcc
    13441 gacggatgcg cgtgatgccg ccatggggat gggtgctgtg ctccgggccg ttgtcggtcg
    13501 tgtagacgac gatggtgtcg tcgccgacgc cgagcttgtc cagcttggcg agcaccgcgc
    13561 cgatgtcgtc gtcgtgctgg atcatgccgg cgccgtgcgg gtcgtcgtag ctcgtgtgct
    13621 cgcgggccct ctccttgtac gcgtccggcg tgaacgtgaa gacgtgcatc ctggacgggt
    13681 tcagccagac gaagaacggc tcgtccgctg ccaccgcccg ctccatgaag tcgaaggcct
    13741 tcccgttgag ctcggcatcg agggtctcga agcgctgctc gtccggcagg cccgtgtcct
    13801 cgacccgctg cttgccgacc tcgccgaaac ggtcgtcggg cacgctggcg tcgtaggtgt
    13861 ccgtggcgaa gctgtggatg atgccgcgcg gctcgaactt cgcgcggaac gcgtcgcttt
    13921 tggggtagtc gttgtcgagg ggctcctgca gcacgttcag gtggtagagg aagccgtaga
    13981 actcgtcgaa gccgtgcacc gtcggcagat gctcgttgcg gtcgcccaga tggttcttgc
    14041 cgaactgccc ggtggcatag ccctgctgct tcagcacctc ggccagcgtc ggcacctcgg
    14101 cccgcatccc ctgcttcgca ccgaccatgc ccaccgtcgt tagccccgag cggatcggat
    14161 acatgccggt gatgaacgag gcccgccccg gcgtgcagct cggctgcgcg tacatgtcgg
    14221 agaagagggc accctcctcg gccagccggt cgatgctcgg cgtccgatag cccctcgagc
    14281 cgcggttgta ggcgctgatg ttccagagcc ccacatcgtc cggcatgatg acgaggatgt
    14341 tcggtctctc ctttgcctga tcctgcgccc ggaccggttc cgccgtcgtg atcagggccg
    14401 cccccacgag ggccgacatc ttcaaggccc gaaggggttc gatcatggcc tcgcctttcg
    14461 tttgctggga aatcgccgcg tctggcgcct ctgcaccgcc tacttcatcg tcaccgccat
    14521 gccccggatc gagccgtcga aggcgaaggg ccgctcatcc gcataggcgg tggacacggg
    14581 cgagcccaga tcgcgcccca cgtcgaacgt ctcggtcgcg gtgaaggcgg ccggaaccgt
    14641 gcgcttcacc gtggtccgcc cggccacctt gccgtcgacg gtgatcacga cctgggcggc
    14701 accgccgggc ttctcgattt cggtatcgat gacgatctcg tgcctgccgc ccgggatagg
    14761 gtcggtggtc accgcgtagt tctcgatcag gagcatgttg tactcgtaga ccagccgacc
    14821 atcccgcatg tagacactga gcccgccgcc ggcgccgccg acggcgtaga gcacgccgtt
    14881 ggcgccgtcc tcgaattccg cgtcgattgt cacggtcgtg ctctggcgcc cgacgccggg
    14941 tgccgtgaat tccggcatgc gttccgtcga gccggtgaac gtccagtggt cataggcggt
    15001 cttcacgcga tcctcggggt ggatgcgcag ccagagcccg gcgccgatgg ggaagtcctg
    15061 gttctctcgg gccacctcga ggaagcgctt cttcatccgc aggagccgct tcggctccgc
    15121 cgccgccaga tcgcgggctt gcgagaaatc cctcccgagg tcgtagagct cccattggtc
    15181 cttggccgaa tcccaatcgg cgaggccttt ggcggagccg gccgtgtccc aggggatcag
    15241 tggcccgaag gtgccagcga accagccgtc catgtagatc ccgcggctgc cgttattgtc
    15301 gaagaactgc tccgacttct gtcccgctgc tccggcatca ccgaacgtgt aggccatgct
    15361 gatgccgtcg agcggcagct gatcatagcc gttgacgacc tttggcggcg tgatctggag
    15421 aatgtcgtaa atggtcggcg ccacgtcgac gaggtgatgg aactgcgggc gcatcctgcg
    15481 gtcatggctg atgtgccccg gccaggagat caccagcggg ttgcgcgtgc cgccgaaata
    15541 gctggcgagc agcttggtgc ccttgaaggg cgtgccgccc gcccaggccc atccggcatg
    15601 gtatatgttg tctgtcttcg ggctgccgag cacggccgtt ccgccgagct tctccagggc
    15661 ttcgagctgc tggtcggtgg tggtctggat gttgttctgg gccaagagct cgctgatcga
    15721 gccctcctga ccctcggccg acgagccgtt gtcgccgaag atgtagaaga tcagcgtgtt
    15781 gtcgcgcagg ccccgcgcct cgagcccgtc gatcagcttg ccgacctgcg tgtcggtatg
    15841 ctcgacatag ccggcgaaga cctccatcag ccgcgtctgg aaatcgaatt gatccgccgg
    15901 gatgctgtcc catgatgcca tcgtcgcgtc gcgcggcgtg agctctgtcc ccgccgggat
    15961 gacccccatg tcgagctgcc gccgccaggt gcgttcgcga taggcgtccc agccatcgtc
    16021 gaaggcgccg tcatacttgt cggcccattc gctgaagatg tggtgcggtc catgcacgcc
    16081 gcccggcgcc cagtacatga agtagggccg ggccggatcg aaggcgcggt agtcgtcgag
    16141 ccaggccagc gcccggctcg ccagatcctc ggtcaaatga taggtcggat cgtcggcggg
    16201 tggctcgaca tggtcgtagt tccgggtcag ccgcggctcc cattgcgagg tctcgccgcc
    16261 gagaaacccg tagaaatgct cgaacccgta ggcgttcggc cagtgatcct tggggccgat
    16321 cgaggtcgtc tcgatcgccg gcgtgttgtg ccacttgccg aaggccgcgg tgtgatagcc
    16381 gtattgcctg aggatctcgg ggatggtcgc cgccgtcttg gggatgaccc ccgtgtagcc
    16441 gtcgaaggcc accgcccgct cggcgatggt gccggagccc acccgcgtgt ggttgcgccc
    16501 ggtgagcagc gccgcccggc tgggcgagca gatcgaggcg gtgttgaagg cgttgtaggc
    16561 gatgccctcc gcggcgagcc gtgcgaaggc gggtgtcctg acctcgccgc cgaacacctc
    16621 ggagatgccg aacccgacat cgtcgagcat cacgatcagg atgtttggcg cgttctcggc
    16681 caggtgccgc gccggctccg gccattgcat cgtcgagtcc tgcagaagct gcttcgcctc
    16741 gcccgccatc ggctcgggcg ggaacggcag cacggagccg tcgtccttct cctgcgtccc
    16801 ggccgatccg gcggccagga gcgacgcgag cgccgccaga atcacgagcc ttgccgtcat
    16861 ttgagcctcc gagatatcga atcaacgaag ccgccgagcc ccaatgagcc ggcgtcgtga
    16921 agaagcgccg cttgcttaca gcagcaccgg cgggtcaagt ccgagaactg ctggaaattc
    16981 ggttttgagg ggtcgcgact gagtgg
//
LOCUS       DAOTFI010000103        16972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4071_length_16972_cov_6.872259, whole genome shotgun sequence.
ACCESSION   DAOTFI010000103 DAOTFI010000000
VERSION     DAOTFI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 16972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16972
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4071_length_16972_cov_6.872259"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="P5558_11950"
     CDS             complement(<1..746)
                     /locus_tag="P5558_11950"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRY25070.1"
                     /translation="MTVRVAINGFGRIGRNILRAIGENGRTDVQVVAINDLAPPATNA
                     HLLRYDSVHGRYPGKVTLEGDVLDIGTGPIKVFAERDMAKLPWGDVGCDIVLECTGIF
                     TSRDKAQGHIDAGARKVLISAPGKDEDITVVYGVNHDKLKAEHTIVSNASCTTNCLAP
                     VAMVLNQTVGIVRGHMTTIHAFTNDQSLLDVFHKDLRRARAATASMIPTSTGAAKAVA
                     LVLPELKGKLDGVAIRVPTQNVSLVDLTFDA"
     gene            complement(777..2759)
                     /gene="tkt"
                     /locus_tag="P5558_11955"
     CDS             complement(777..2759)
                     /gene="tkt"
                     /locus_tag="P5558_11955"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY25071.1"
                     /translation="MTDRAVTHADLANAIRALAMDAVERAKSGHPGMPMGMADVATVL
                     FTRHLRFSAAHPAWPDRDRFVLSAGHGSMLLYALLHLTGHADMTIDELKRFRQLGSKT
                     AGHPEYGHAAGIETTTGPLGQGLATAVGMAMAERLLAVRHGDDLVDHRTYVIASDGDL
                     MEGISHEAVSLAGHLRLGKLIVLFDDNGISIDGPTSLSCSDDQLARFEASGWHAIAID
                     GHDPAAIDSAIDAAKADARPSLIACRTTIGLGAPTKAGKSSSHGSPLGAAEIEGARKA
                     LGWDHPPFVVPEPIQAAWRAAGERGESARHDWEARLQAAPDDVRAQFLRTLEGHLPDG
                     LKAAVEAMKTRLFAERPEIATRKSSQNALALLTELVPELIGGSADLTGSNNTDTRSTP
                     PLDPVTFRGRHIHYGIREHGMVAAMNGMALHRGIIPYGGTFLIFTDYARPAIRLAALM
                     RQRVVVVATHDSIGLGEDGPTHQPIEQLASLRAIPNLLVFRPADAIEVLECWHAALEH
                     GTGPSVLALTRQNLPTLRARDEAENLAARGAYVLAEAEGQRAATIIATGSEVAIAMEG
                     RALLAAGGLAVAVVSMPCMELFERQDADYRASVLGNAPRIAVEAASPFGWTRYVQSEE
                     HVVGMTSFGASAPIDDLYAHFGITAAAVAERVRALA"
     gene            3012..3278
                     /locus_tag="P5558_11960"
     CDS             3012..3278
                     /locus_tag="P5558_11960"
                     /inference="COORDINATES: protein motif:HMM:NF025122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HRY25072.1"
                     /translation="MPSIDEAQARLEAALARLEQAIAARRAQAPAADVALVGEVEGLR
                     DECSALRERLANAEARHARLKGSMTAMGGRLDAAISELDSLVEG"
     gene            3321..3650
                     /locus_tag="P5558_11965"
     CDS             3321..3650
                     /locus_tag="P5558_11965"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRY25073.1"
                     /translation="MPGLEILIHGRTYGVQCGEGEELRLKRLAAYVDARVREQAAAHG
                     QIGDARLLVLTSLLIADELDDALSEIKRLQGGVSRAEAADDGATAALIERLADRLEQL
                     AAAVETT"
     gene            3658..3817
                     /gene="ssrS"
                     /locus_tag="P5558_11970"
     ncRNA           3658..3817
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P5558_11970"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3871..4476
                     /locus_tag="P5558_11975"
     CDS             3871..4476
                     /locus_tag="P5558_11975"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRY25074.1"
                     /translation="MSRRPEPRPGKPEAVLAEKRRLRQAAMARRDGLADDVRGEASRQ
                     ALVRLVALLPLEPTSVVAAFWPLGSEIDCRQLFRAMGALGIATCLPRMTGRTEALVFH
                     RYRLGDGLLEGPMRVLEPLAEAPVCIPTIVVVPLVAFDRQGHRLGYGGGYYDRTLAAL
                     GDGVEAIGLAFAAQEVPLLPALPTDRCLRAIVTESAVRHFR"
     gene            4503..5321
                     /locus_tag="P5558_11980"
     CDS             4503..5321
                     /locus_tag="P5558_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRY25075.1"
                     /translation="MRILHVGDVVGRAGRALVKAELPGLRERLALDMVVVNAENAAGG
                     FGLTPAIGDELLAAGADLLTLGNHAWDQRDLIGHIGNEPRIVRPLNMQPGTPGQGIAQ
                     FRTARGRKVVVLQVLGRLFMGLYGDPFRHLEEALASWFLGGNADAIIVDVHAEATSEK
                     TAIGHFLDGRVTLVAGTHTHVPTADHRVLAGGTAYISDVGMTGDYDSVIGMDKAISLQ
                     RWRSSVPGPRLQPADGAATLCAVLVETDPKTGLATRIEPVRRGGALVPAEPHRG"
     gene            complement(5275..5550)
                     /locus_tag="P5558_11985"
     CDS             complement(5275..5550)
                     /locus_tag="P5558_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRY25076.1"
                     /translation="MGRRIAFDRPIATLDELEHELRTPLTSILSISELLRDHPDMSES
                     ERRRFVDAMVDEGERLAALIELLLASGRLGAVLSRDAARPAPGRHPA"
     gene            complement(5678..7417)
                     /gene="ggt"
                     /locus_tag="P5558_11990"
     CDS             complement(5678..7417)
                     /gene="ggt"
                     /locus_tag="P5558_11990"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY25077.1"
                     /translation="MHVRAILALSVLAIGTAHAEAPQQPESTIAIAESAAVTSSTFMV
                     SAAHPEASRVGQAVLAAGGNAIDAIVAVQFMLNLVEPQSSGIGGGSFLLYWDAAEERL
                     WTFDGRETAPAAAGPDLFLDPATGEPMAFWDAVVGGRSVGVPGTLKLLETVHERFGSR
                     PWAELVEPTIELAEAGFTVSPRLAGAIAEAIEDDLGRFAATREYFLDADGAALAAGST
                     LANPAFAATLRQIAAEGSAPFYTGDLASRIVAVVTGAEGNAGQMSLDDLAGYEVLTRD
                     PICVDYRRNAVCGMGPPSSGGLTVGQILGLLGHVDMPGLGYSPTAVHLFLEASRLAFA
                     DRALYMADADFVPMPIAGLLDPTYLMLRAQALDFDASMGKARAGNPPWRDARVLAPQD
                     QPEPGGTTHVSIVDSAGNAVSFTSSIETGFGSRLMVGGFLLNNQLTDFSFRPMAGGRP
                     VANRVEPGKRPRSSMAPTIVFGPDGEIRLVVGSPGGARIIDYVAQTLVGVLDWGMNIQ
                     AAIDLGHFTNLNGDTTLEAASGAEALAGALEALGHEVKIADQNSGLHGIERLPEGWRG
                     GADPRREGIVLGD"
     gene            7520..8707
                     /locus_tag="P5558_11995"
     CDS             7520..8707
                     /locus_tag="P5558_11995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRY25078.1"
                     /translation="MTSYPTIKIQGGRDRRINTGAPWLYSNELRMDDDAKALTPGSLV
                     RLMAPSGKIVGVAQFNPHSLIAARVMTRNKDAVVDAKFLERRIARALAVREQLYATSH
                     YRLVHAEGDGLPGLVIDRYGDLLVVQLNTAGMQALVEPLVAALDDVLRPQVILARNDT
                     SGRAMERLAGEDTVLRGALPERISIEENGLAFAVAPGDAQKTGWFYDQSMNRAFAGRL
                     ARGAKVLDLYSYSGGFGLTALARGAKSVLAVDRSASALALAAESARAQGVADRFETRT
                     GDVFAAIDALAAEKARFELVIADPPAFVRAKKDLPVGLRGYRKLARDAAGLVSEAGFL
                     CIACCSHNVSPEAFATEVFAGIKAAGRGARLLHAAGAAPDHPIHPALPETAYLKFLAY
                     ALD"
     gene            8795..10930
                     /locus_tag="P5558_12000"
     CDS             8795..10930
                     /locus_tag="P5558_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HRY25079.1"
                     /translation="MSRFRAGGAERAPEPAAPNTLVASFARLCAQLDRPLPEAELQSI
                     AGGGRQADLASLVRLGDRQGLDIKVLPATRLRLETLRAPFLLVGEDGARSWLIRARIK
                     TRLLLVDPLSGTTRPVSAGELEARGARILVPRVRRAAGADGRALWREAVWQRLRPVFL
                     EVGLASVCINLLALAMPLFMMTVYNKVIGQGSVGTLDVLAIGMVSLFGFELVLRSLRG
                     YVVSHTGAKLDLALSSEVVHHLLRLPYRVFEAMPSGQLMERLRQLDQLRTFLSGNLPL
                     LLVDFAFVGLFIAAIFVFSPPLGWVTLLALPLFLGLSALAQVRQSRLVRANFRATAKK
                     STGLAETVAQALTVKALGLESEMQRRFEDRQVESAWAGFRASRLANGVAATGQVFQHL
                     VSLLVVYLGARMIVSGEMSIGALVACSILSARALAPMRQIFFAWHQLQQARDAMARLG
                     DLMREPAEDSGTSLPLPNPLRGRFRMEEVRFGYDPAKPSALDGLDLEIEPGCMLAVVG
                     PPGSGKSTLAKLLIGLERPQSGRVLVDDVDIARLEHAAFRAQLGVVPQEIQLFAGTIA
                     ENIGIASVDRSLNRIIAAAKFVGIHDTIQRLPDGYDTQLGERGNGLSLGQRQLLCIAR
                     ALVRNPRILVLDEATSALDLASEAYLLRNLRRAGSGRTIVMITHRLSVIEMCDRAILL
                     EAGRLVMAGAPAEVARRARQPVAHDAARA"
     gene            10944..12302
                     /locus_tag="P5558_12005"
     CDS             10944..12302
                     /locus_tag="P5558_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRY25080.1"
                     /translation="MGKVSNVPMRPAAVTGEDADLLPDDIRAFLEQRRSPVAGLLIGS
                     VALLLTAGLAWTAWARIDEVVRASGRVEPVARVKLINHPHGGRLATLEVAEGDRVEAG
                     RVLLTFAPEIDAKEHAELLGRWHVRTAEIQRLQAEVDGSDLAAAEELEAARPDLAEQA
                     RTLLAARRAARDGDREALERNVEGHQSALRGAEAEVARLELGGRLLEEQFRAVRELAE
                     RGLYPRLKMMDIERQLADSRAELVKAETAVVSARAALAESRSRLASFDKDWRRHLLES
                     LAEALAERDRLTEQLGAKTAMLDELVLTAPVDGIVQELKPAVVGQSVAANEAILKLVP
                     TGEGIVVNALVANADIGRIAIGMPATIKVRAYEFARFGAIEGTVEQISADAIAAETPG
                     EPPSFAVRIATDQDQLAPDGSLPVLPGMAVDVEIRIGERTILSYFTDSLTRVKERAFK
                     DG"
     gene            complement(12315..>12591)
                     /locus_tag="P5558_12010"
     CDS             complement(12315..>12591)
                     /locus_tag="P5558_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25081.1"
                     /translation="TPLMRAITQTEILEETDERLVLRLRYTYQDRSFSSVEVDALGFI
                     QPQSCVGFATRTFTISVGPEGGAAEVLEMSGPQRDIRKNFGVGGRTP"
     assembly_gap    12592..12601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12767..14164)
                     /locus_tag="P5558_12015"
     CDS             complement(12767..14164)
                     /locus_tag="P5558_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY25082.1"
                     /translation="MRPPGDRPPWGRSLSARLLLLTVGFLLLGEILIFVPSIARFRHV
                     YLEERIAAAHLATIGVAARGQPPSLALADALLSHAGVLSITIERERPVLMLGMLPPVE
                     RTIDITDRDWVGLILEAFSTLYHGGQRVVRVVGPSPMEYGTTVDITLTEAMLHAEMID
                     YARRIILLSFALSLIVGLLLLASLRSLIVRPLARITAKIAHFRKRPEDSSLDTPASRR
                     SDEIGIVDREVDAMTRDLRQALAQKTRLAALGEAVGKLHHDLRNILATAFLLSDRLEG
                     SADPAVRAVTPRLVQTLERAIRLCQETLDFARSRPPRPSTAPVALRALVDEVTTLLSP
                     AGTPTTIVNEIAPDVMVVADPDQLHRLFLNLARNALEAMPGGGQLTFRNRRVADRFVV
                     EVEDTGHGLPETVRDHLFEPFTTSSSGNGAGLGLAICREIARAHGGDLQVRKSDDSGT
                     VMTLTLPAMAGSVGT"
     gene            14466..14600
                     /gene="rpmH"
                     /locus_tag="P5558_12020"
     CDS             14466..14600
                     /gene="rpmH"
                     /locus_tag="P5558_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRY25083.1"
                     /translation="MKRTYQPSRLVRKRRHGFRARMATVGGRRVIRDRRAKGRKRLSA
                     "
     gene            14627..15034
                     /gene="rnpA"
                     /locus_tag="P5558_12025"
     CDS             14627..15034
                     /gene="rnpA"
                     /locus_tag="P5558_12025"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRY25084.1"
                     /translation="MLRRLEKRRDFLRVARGRKAVRPAFVVQMAPAPGPVSGDVDDGD
                     VATEAWRIGFTASRKVGGAVQRNRAKRRLRAVARAVMPELAHRGFDYVLVARTPVLTC
                     PYSRLEADLTAALKELARWARPCSGDVLRGRSI"
     gene            14929..15324
                     /gene="yidD"
                     /locus_tag="P5558_12030"
     CDS             14929..15324
                     /gene="yidD"
                     /locus_tag="P5558_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRY25085.1"
                     /translation="MPIQPTGSRSDGGSEGTGTLGAAVQWRRLARAVDLIVASFLRLL
                     VHAYRYLLAPVLPRGCRFAPSCSLYALEALARHGAITGGGLALRRLASCHPWGGSGYD
                     PVPAAAPRRGCRCPDSLPAASQEPIGQQR"
     gene            15321..>16972
                     /gene="yidC"
                     /locus_tag="P5558_12035"
     CDS             15321..>16972
                     /gene="yidC"
                     /locus_tag="P5558_12035"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRY25086.1"
                     /translation="MTDQRNLILAIVISVGIILGFQVFYEMPRMERQQQLTESQDTGQ
                     GSEGVVPPSPTSSGAASGGAPASSAGVGAALDPNAGLATSRAEALARLPRVQIANGRV
                     HGSLALDGVRFDDVTLVDYRQTVDPNSPEIELLNPVGARDTYFAEFGWVPEGEIVVPN
                     AETLWEQVGDKLTQEVPVEFRWDNGQGLLFAKQVAVDENFMFTLERSVTNTSDQPVTL
                     FPYGLLSRWGTPKTAGFYILHEGPIGVLDGTLKEIDYDDLHEEPITLSSTGGWAGFTD
                     KYWLTSLVPDQAEELQANFRHFVRDGQDRYQVDYLGTARVIQPGATITVTDRLFAGAK
                     EVRLLDAYSETYGIPLFDRAVDFGWFYFLTKPFFYILTFFYHNVFGNYGLAILALTLL
                     VKIVFFPLANKSYRSMSQMKKLQPEMVKLRERHANDKQKMQQELMALYKREKVNPMAG
                     CLPILLQIPVFFALYKVLFVSIEMRHAPFFGWIHDLSAPDPTSIFNLFGLLPFAVPAF
                     LMIGVWPVLMGVTMWLQFRLNPQPADPIQAKVMGLLPFIFVFL"
BASE COUNT         2504 a         5663 c         6095 g         2700 t
ORIGIN      
        1 gcatcgaagg tgagatcgac gaggctgacg ttctgcgtcg gcacgcggat ggccacgcca
       61 tcgagcttgc ccttgagctc cggcaggacg agggccacgg ccttggcggc accggtcgag
      121 gtcgggatca tcgaggcggt ggcggcacgg gcccggcgga ggtccttgtg gaacacgtcg
      181 agcaggctct ggtcgttggt gaaggcgtgg atggtcgtca tgtgcccgcg gacgatgccg
      241 acggtctggt tcaggaccat ggcgaccggg gccaggcagt tcgtggtgca ggaggcgttg
      301 gagacgatcg tgtgctcggc cttcagcttg tcgtggttga cgccgtagac gacggtgatg
      361 tcctcgtcct tgccgggagc ggagatcagc accttcctcg caccggcgtc gatatggccc
      421 tgggccttgt cgcgggacgt gaagatgccc gtgcattcga gcacgatgtc gcagccgacg
      481 tcgccccagg ggagcttcgc catgtcccgc tcggcgaaga ccttgatcgg gccggtgccg
      541 atatcgagca cgtcgccctc gagggtcacc ttgcccggat agcggccatg aacgctgtca
      601 tagcgcagga gatgcgcgtt ggtggcgggg ggagccagat cgttgatcgc cacgacctgc
      661 acgtcggtac ggccattctc gccgatggcg cgcaggatgt tgcgaccgat tcgaccgaaa
      721 ccgttgatcg cgacgcggac agtcatctgt cgttctccct caggtgtgca ggccgttcag
      781 gcgagtgccc ggacgcgttc cgcgaccgca gccgccgtga tgccgaaatg ggcgtagagg
      841 tcgtcgatcg gcgcgctggc gccgaagctc gtcatgccga cgacgtgctc ctcgctttgc
      901 acgtagcgcg tccagccgaa aggtgaagcg gcttcgacgg caattcgcgg cgcgttgccg
      961 agaaccgatg cgcgatagtc ggcatcctgc cgctcgaaga gctccatgca cggcatggag
     1021 acgacggcga cagcgaggcc cccggccgcc agcagcgccc tgccttccat ggcaatggcc
     1081 acttccgagc cggtcgcgat gatcgtggcg gcccgctgtc cttcggcctc ggccaggaca
     1141 taggcgccgc gcgccgcgag gttctcggct tcgtcccttg ccctgagcgt gggcaggttc
     1201 tgcctggtca gggcgaggac cgatggtccg gtcccgtgct cgagcgccgc atgccagcac
     1261 tccagcacct ctatggcgtc ggccgggcgg aagaccagga ggttggggat ggccctgagc
     1321 gaggcgagct gctcgatcgg ctggtgggtc ggcccgtcct cgccgaggcc gatggagtcg
     1381 tgggtcgcga cgaccacgac ccgctgccgc atcagggccg cgaggcggat cgccggccgg
     1441 gcgtagtcgg tgaagatgag gaacgtgccg ccatagggga tgatgccgcg atgcagcgcc
     1501 atgccgttca tggcggccac catgccgtgc tcgcggatgc cgtagtggat gtggcggccc
     1561 cggaaggtga cgggatcgag cggcggcgtg ctcctggtgt cggtgttgtt cgagccggtg
     1621 aggtcggccg agccgccgat cagctcgggc accagctcgg tcaggagggc caacgcattc
     1681 tggctcgact tgcgggtggc gatctcgggc cgctcggcga agagccgggt cttcatggcc
     1741 tcgacggcgg ccttcaggcc gtccggcagg tggccttcca gggtgcgaag gaactgggcc
     1801 cggacatcgt cgggtgcggc ctgcagccgc gcctcccagt cgtggcgggc gctctcgccc
     1861 cgttcgccgg cggcccgcca ggccgcctgg atcggctcgg gcacgacgaa gggcgggtga
     1921 tcccagccca aggccttgcg cgcgccctcg atctcggcgg caccgagcgg cgagccgtgg
     1981 ctcgaggatt tcccggcctt ggtcggcgca cccaggccga tggtggtgcg gcaggcgatc
     2041 aggctcggcc gggcgtcggc cttcgcggca tcgatggcac tgtcgatcgc cgccggatcg
     2101 tggccgtcga tggcgatcgc gtgccagccg ctggcttcga accgcgccag ttgatcgtcg
     2161 gagcaggaca gcgaggtcgg cccgtcgatc gagatgccgt tgtcgtcgaa gagcacgatc
     2221 agcttgccga gccggagatg gccggcaagc gacaccgcct cgtggctgat gccttccatc
     2281 agatcgccgt cgctggcgat cacataggtc cggtggtcga cgaggtcgtc gccatggcgc
     2341 acggccagga ggcgttccgc catggccatg ccgacggcgg tcgccagacc ctggccgagc
     2401 gggccggtcg tcgtctcgat cccggcggcg tgcccgtatt cggggtgtcc cgcagtcttc
     2461 gagccgagct ggcgaaagcg cttcagctcg tcgatcgtca tgtcggcatg ccccgtgagg
     2521 tggagcaggg catagagcag catcgagccg tggcccgcgg agagcacgaa ccggtcgcgg
     2581 tccggccacg ccggatgcgc cgccgagaag cggaggtggc gggtgaagag gaccgtcgcg
     2641 acgtccgcca tgcccatggg catgccggga tgcccggatt tggcccgctc gacggcatcc
     2701 atggccagcg cccggatggc gttggcgaga tctgcgtggg tgacagcccg gtcggtcatc
     2761 gttcctcaac tcgctgcacg gaagccagct tcctcgccgg tgatccgatc ggcctcggca
     2821 tcacgcgcga tcccgcctga caagggcacc gacacggcta tcgacgccgc ggacactggc
     2881 cgaaccaccg gaaaaatcaa cggccatttg gtcacgggcc gacaggctga ccacccggtc
     2941 tggttgacgg gcgcgggtgg ctatgtcatc gagcggcaac cattcagtgt caaccgcgtg
     3001 aataaggcga catgccttcc attgatgaag ctcaagcccg cctcgaggca gcgctggccc
     3061 gactcgagca ggcgatcgcc gcccgtcggg cgcaggcgcc cgcggccgat gtcgccctgg
     3121 tcggcgaggt cgagggcctg cgcgacgagt gctcggcgct gcgcgagcgg ctggccaatg
     3181 ccgaggcgcg gcatgcgcgc ctgaagggct cgatgacggc catggggggg cggctcgatg
     3241 ccgcgatctc cgagctcgat tcgcttgtcg agggttgagg cggggttgaa cggcatgtcc
     3301 agggggggcg gcgcatagca atgccagggc tcgagatcct cattcacggc cgcacctacg
     3361 gcgttcaatg cggcgagggc gaggagctgc ggctcaagcg gctcgccgcc tatgtcgacg
     3421 cgcgggtcag ggagcaggca gcggcgcacg ggcagatcgg cgatgcccgg ctcctcgtcc
     3481 tgaccagcct gctcatcgcc gacgagctcg acgacgcgct ttccgagatc aagcggctgc
     3541 agggcggcgt cagtcgcgcc gaggccgccg acgacggcgc cacggctgcg ctgatcgaac
     3601 gtctcgcaga tcgactggag cagcttgcgg cggcggtcga gacgacctaa ctacggcttg
     3661 gagctacccg gtgcgtcagc gtcttcatca cgctctgggc ctaatgtgtt cctacgggag
     3721 ctgtctctgt ccgggccctg gtccgggtat cacggcgccc acctgctcgt caggccccag
     3781 ggacatagtc gatgtcaacg gccagggtgg ctccgcttct cccttcatcg atcgtccgcc
     3841 cgaatgccat ctcgaccgct tcgggtcgag atgagccgcc gaccagagcc gcgaccgggg
     3901 aagccggaag ccgttctggc cgagaagcgc cgcctgcggc aggcggcgat ggcgcggcgg
     3961 gacgggctcg ccgacgacgt tcgcggggag gccagccggc aggcgctcgt ccgcctcgtg
     4021 gctctattgc cgctcgagcc gaccagcgtc gtcgcggcct tctggccgct cggctccgag
     4081 atcgactgtc ggcagctctt tcgggccatg ggggcactcg gcatcgccac ctgcctgccc
     4141 cggatgaccg gccggacaga ggccctcgtc ttccatcgct accggttggg cgacggcctg
     4201 ctcgaggggc cgatgcgggt cctcgagccc ctggccgagg cgccggtgtg cattccgacc
     4261 atcgtcgtcg tgccgctcgt cgccttcgat cgccagggcc accggctcgg ctatggcggc
     4321 ggctattacg accggaccct ggcggcgctg ggtgacgggg tcgaggcgat cgggctggct
     4381 ttcgctgccc aggaggtgcc gctgctgccg gcgttgccga ccgaccggtg cttgcgggcc
     4441 atcgtgacgg agtccgccgt gcgccatttc aggtgatctc tagtgatgtt ggaaatcgaa
     4501 ccttgcgcat tctccatgtc ggtgacgtcg tcggccgggc cggccgggca ctggtcaagg
     4561 ccgagctgcc cggcctgcgg gagcgcctcg ctctcgatat ggtcgtggtc aacgcggaga
     4621 acgcggccgg cggcttcggc ctgaccccgg cgatcggcga cgagctgctg gcggccggcg
     4681 ccgatctcct gaccctcggc aatcatgcct gggaccagcg ggacctgatc ggccatatcg
     4741 gcaatgagcc gcgcattgtc cgcccgctga acatgcagcc cggcacgccg gggcagggaa
     4801 tcgcccagtt ccgcaccgcg cgcggccgca aggtggtggt gctgcaggtc ctgggccgcc
     4861 ttttcatggg cctttacggc gatccgttcc ggcatctgga ggaggcgctc gcctcctggt
     4921 tcctcggcgg caatgccgat gccatcatcg tcgacgtcca cgccgaggcg acgagcgaga
     4981 agacggcgat cggtcatttc ctcgacggcc gggtgaccct cgtcgccggc acccataccc
     5041 acgtgccgac ggcagaccac cgggtcctcg cgggcggcac ggcctatatc agcgatgtcg
     5101 ggatgaccgg cgactacgac agcgtgatcg gcatggacaa ggcgatctcg ctgcagcgct
     5161 ggcgctccag cgtgccgggc ccacgattgc agccggcgga tggggcggcg accctttgcg
     5221 ccgttctggt cgagaccgat ccgaagactg gtctcgccac gcgtatcgag ccggtcaggc
     5281 ggggtggcgc cctggtgccg gccgagccgc atcgcggctg agcaccgcgc cgaggcggcc
     5341 gctggccagc aggagctcga tcagggcggc gaggcgctcg ccctcgtcga ccatggcatc
     5401 gacgaagcgg cggcgctcgc tctcgctcat gtcggggtgg tcgcggagca gctcgctgat
     5461 cgagaggatc gaggtcagcg gtgtgcgcag ctcgtgctcg agctcgtcga gcgtggcgat
     5521 cggtcgatcg aaggcgatcc tgcggcccat tccggtcctc tcgggttgcc gcctcgtggg
     5581 ctggatccga gataacaccg gcaggtcccg ggggaaggtg ccggtcgacc actgcggcgt
     5641 ttgatcgctc accgggtggc gagggatgag cagatgttca atcgcccagc acgatcccct
     5701 cccggcgcgg gtcggcgccg ccgcgccagc cttcgggcag tcgttcgatg ccgtgcaggc
     5761 cgctgttctg gtcggcgatc ttgacctcgt gacccagcgc ctcgagcgcc ccggccagcg
     5821 cctcggcacc gctcgccgcc tcgagggtcg tgtcgccgtt gagattggtg aagtggccga
     5881 ggtcgatcgc cgcctggatg ttcatccccc agtcgagcac gccgaccagg gtctgggcga
     5941 catagtcgat gatgcgcgcc ccgccgggcg agccgacgac gaggcggatt tccccgtccg
     6001 gaccgaagac gatggtcggc gccatcgagc tgcgcggtcg cttgccgggc tcgacccggt
     6061 tggcgacggg ccggccgccg gccatcgggc ggaaggagaa atcggtgagc tggttgttga
     6121 gcaggaagcc gccgaccatc agccggctgc cgaagccggt ctcgatcgag ctggtgaagg
     6181 agacggcatt gccggcagag tcgacgatcg agacatgggt cgtgccgccg ggctccggct
     6241 ggtcttgcgg ggccagcacg cgagcgtcgc gccagggcgg gttgccggcc cgggccttgc
     6301 ccatgctggc gtcgaaatcg agcgcctggg cgcgcagcat gagataggtg gggtcgagca
     6361 ggccggcgat cggcatcggc acgaagtcgg cgtcggccat gtagagggcg cggtcggcga
     6421 aggcgagcct cgatgcctcg aggaagagat gcaccgcggt cggcgagtag ccgaggcccg
     6481 gcatgtcgac atgcccgagc aggcccagga tctgacccac ggtgagcccg cccgaggagg
     6541 gcggccccat cccgcagacg gcattccgtc gatagtcgac gcagatcggg tcgcgggtga
     6601 ggacctcata gccggcgagg tcgtcgaggc tcatctggcc ggcattgccc tccgcaccgg
     6661 tcacgacggc gacgatccgg gatgcgaggt cgcccgtgta gaagggggca ctgccctcgg
     6721 ccgcgatctg gcggagggtg gcggcgaagg cggggttggc gagcgtgctg ccggcggcga
     6781 gggcggcacc gtcggcatcg aggaagtact cgcgggtagc ggcgaagcgg ccgagatcgt
     6841 cctcgatggc ctcggcgatg gcaccggcga ggcgcggcga caccgtgaag ccggcttccg
     6901 ccagctcgat ggtcggctcg acgagctcgg cccatggccg gctgccgaag cgctcgtgga
     6961 cggtctcgag cagcttcagg gtgcccggca cgccgaccga ccggccgccg acgacggcat
     7021 cccagaacgc catgggctcg ccggtcgcgg gatcgaggaa gagatcgggg ccggccgctg
     7081 ccggggccgt ctcgcggccg tcgaaggtcc aaaggcgctc ctcggcggca tcccagtaga
     7141 ggaggaagct gccgccgccg atgccggacg actggggctc gaccagattc agcatgaact
     7201 gcacggccac gatggcgtcg atcgcgttgc cgccagcagc gaggaccgcc tggcccaccc
     7261 gcgaggcctc gggatgggcg gcgctgacca tgaaggtcga gctggtgacc gccgcggact
     7321 cggcgatggc gatggtcgat tccggctgct ggggcgcttc ggcatgggcg gtgccgatag
     7381 ccaggaccga tagggcgagg atggcgcgaa cgtgcatgga tgcctccctt ggtgatcgcg
     7441 agcccgacaa gccgggcaaa gcgcgcttgc tgctcatgac cggctcgggc atgtaggctg
     7501 gccaacccgg gaacgcaaaa tgacctccta tccgacgatc aagattcaag gcggccgtga
     7561 ccgccggatc aataccggcg caccgtggct ctactcgaac gagctgcgca tggacgacga
     7621 cgccaaggcg ctgacgccgg gcagcctcgt ccggctgatg gcgccatcgg ggaagatcgt
     7681 cggcgtggcg cagttcaacc cgcactcgct gatcgcggcc cgcgtgatga cccgcaacaa
     7741 ggacgcggtg gtcgacgcga aattcctcga gcgccggatc gcccgggccc tggccgtgcg
     7801 cgagcagctc tatgcgacat cgcattatcg gctggtgcat gccgagggcg acggcttgcc
     7861 cggcctcgtg atcgatcgct acggcgatct cctggtcgtc cagctcaaca cggccggcat
     7921 gcaggccctg gtcgagcccc tggtcgccgc cctggacgat gtgctgcggc cgcaggtgat
     7981 cctcgcccgc aacgacacgt caggcagggc catggagcgg ctcgccggcg aggacaccgt
     8041 gctgcggggc gccctgccgg agcgcatctc gatcgaggag aacggcctcg ccttcgccgt
     8101 ggccccgggt gacgcgcaga agacgggctg gttctacgac cagtcgatga accgcgcctt
     8161 tgccggccgt ctggcccggg gtgccaaggt gctcgatctc tacagctact cgggcggctt
     8221 cgggctgacc gcgctcgcca ggggggcgaa gtcggtgctc gccgtggacc ggtcggccag
     8281 tgctttggcg ctcgccgcgg aaagtgcccg ggcgcagggt gtggcggacc gcttcgagac
     8341 gcgcaccggc gacgtcttcg ccgcgatcga cgcgcttgcg gccgagaagg cacggttcga
     8401 gctggtgatc gccgatccgc cggccttcgt ccgggcgaag aaggatctgc cggtggggct
     8461 gcgtggctat cgcaagctcg cccgcgacgc ggcgggcctg gtcagcgagg ccgggttcct
     8521 ctgcatcgcc tgctgctcgc acaacgtgtc gcccgaggcg ttcgccaccg aggtgttcgc
     8581 cggcatcaag gccgccgggc gcggtgcgag gctgctgcat gccgcgggtg cggcgccgga
     8641 ccacccgatc cacccggccc tgccggagac ggcctatctg aagttcctcg cctatgcgct
     8701 ggattgagta attcaactgt tggttgttga tcgccgtcat atttagcaat atacgttagc
     8761 tcgatccgtg gcgtgaacag ccgaggtcga gtcattgtcg agattcagag ccggcggcgc
     8821 cgagcgagcg cccgaaccgg cagcgcccaa cacgctcgtc gcgagcttcg cccggctgtg
     8881 cgcccagctc gaccggccgc tgcccgaggc cgagctgcag agcatcgccg gcggcggccg
     8941 gcaggcggac ctggcatcgc tcgtccggct cggcgaccgg caggggctcg acatcaaggt
     9001 gctgcctgcc acccggctgc gcctcgagac gctgcgcgca cctttcctgc tggtcggcga
     9061 ggacggtgcc cggtcctggt tgatccgggc gcggatcaag acccggctcc tgctggtcga
     9121 tccgctcagc gggaccaccc ggccggtgag cgccggcgag ctggaggcca ggggtgcccg
     9181 catcttggtt ccgcgggtcc gccgtgccgc cggggccgat ggccgcgccc tctggcgcga
     9241 ggccgtctgg cagcgcctgc ggccggtctt cctcgaggtc ggcctggctt cggtctgcat
     9301 caatctcctg gccctcgcca tgccgctctt catgatgacc gtctacaaca aggtgatcgg
     9361 ccagggctcg gtcggcacgc tcgacgtcct cgccattggc atggtgtcgc tcttcggctt
     9421 cgagctcgtg ctgcgttccc tgcgcggcta cgtcgtcagc cacaccggcg ccaagctcga
     9481 cctcgccctg agcagcgagg tggtccacca cctgctgcgg ctgccgtacc gtgtcttcga
     9541 ggcgatgccg agcggccagc tcatggagcg gttgcggcag ctcgaccagc tccgcacctt
     9601 cctctccggc aacctgccct tgctgctggt cgacttcgcc ttcgtcgggc tcttcatcgc
     9661 cgccatcttc gtcttcagcc cgccgctcgg gtgggtgacc ttgctggcgt tgccgctctt
     9721 tctcggcctt tcggcactgg cgcaggtccg ccagagccgg ctcgtccggg ccaatttccg
     9781 cgccaccgcg aagaagtcga ccggcctcgc cgagaccgtg gcccaggcgc tgaccgtgaa
     9841 ggcgctgggt ctggaatccg agatgcagcg acgtttcgag gaccggcagg tggagagtgc
     9901 gtgggccggg ttccgggcca gccggctggc caacggggtt gccgcgacgg gccaggtctt
     9961 ccagcatctg gtgagcctgc tggtcgttta tctcggggca aggatgatcg tctcgggcga
    10021 gatgagcatc ggggcgctcg ttgcctgcag catcctctcc gcccgggcgc tggcgccgat
    10081 gcgccagatc ttcttcgcct ggcaccaatt gcagcaggcg cgggacgcca tggcgcggct
    10141 cggcgacctg atgcgcgagc cggccgagga ttcgggcacc agcctgccgc tgcccaaccc
    10201 gctgcgcggc cggtttcgca tggaggaagt ccgcttcggc tacgacccgg cgaagccgtc
    10261 ggcgctcgac gggctcgacc tcgagatcga gcccggctgc atgctcgcgg tcgtcggccc
    10321 gcccgggtcc ggcaagagca cgctggccaa gctgctcatc ggcctcgaac ggccgcaatc
    10381 gggtcgcgtg ctggtcgacg acgtcgacat cgcaaggctc gagcacgcgg cctttcgcgc
    10441 ccagctcggg gtcgtgccgc aggagatcca gctgttcgcc gggacgatcg ccgagaacat
    10501 cggcatcgcc tcggtcgacc ggtcgctcaa ccggatcatc gcggcggcca agttcgtcgg
    10561 catccacgac acgatccagc gcctgcccga cggctacgac acccagctcg gcgagcgcgg
    10621 caacggcctg tcgctcggcc agcgccagct cctctgcatc gcccgggccc tggtccgcaa
    10681 cccgcgcatc ctggtcctcg acgaggcgac gagcgcgctc gacctggcga gcgaggccta
    10741 tctcctgcgc aacctgcgcc gggccggcag cggccgcacg atcgtcatga tcacccaccg
    10801 cctgtcggtc atcgagatgt gcgaccgggc gatcctgctc gaggccggcc ggctggtgat
    10861 ggccggcgca cccgccgagg tggcccggcg ggcgcggcag ccggtggcgc atgatgcagc
    10921 cagggcatga ggggacacgg ttgatgggca aggtctcgaa cgtcccgatg cggcctgcgg
    10981 ccgtgaccgg cgaggacgcg gatctgctgc cggacgacat ccgggccttt ctcgagcagc
    11041 gccgctcgcc tgtcgcgggt ctgctgatcg gcagcgtcgc cctgctgctc acggccgggc
    11101 tggcctggac cgcgtgggcg agaatcgacg aggtggtgcg cgcctcgggc cgggtcgagc
    11161 cggtggcgcg ggtcaagctc atcaaccatc cgcatggcgg ccgcctggcg acgctggagg
    11221 tcgccgaggg ggatcgggtc gaggcgggac gggtgctctt gaccttcgcg ccggagatcg
    11281 acgccaagga gcatgccgag ctgctcggcc gctggcatgt ccgcacggcc gagatccagc
    11341 ggctgcaggc cgaggtcgat ggcagcgacc tggctgctgc cgaggagctc gaggcggcgc
    11401 gccccgacct cgcggagcag gcgcggaccc tgctggcggc gcggcgcgcg gcccgcgacg
    11461 gcgatcgcga ggcgctcgag cggaatgtcg agggccacca gtcggcgttg cgtggtgccg
    11521 aggcggaggt ggcccggctc gagctcggcg gccggctgct ggaggagcag ttccgggccg
    11581 tccgcgagct cgccgagcgg gggctctacc cgcgcctcaa gatgatggat atcgagcgac
    11641 agctggcgga tagccgggcg gagctggtca aggccgagac ggcggtggtc tcggcgcggg
    11701 ccgcgctcgc cgagagccgg agccggctcg ccagcttcga caaggactgg cgccgacatc
    11761 tgctcgagag cctggccgag gccctggccg agcgggatcg cctgaccgag cagctcgggg
    11821 cgaagacggc catgctcgac gagctggtgc tgaccgcacc ggtcgacggc atcgtccagg
    11881 agctcaagcc ggcggtggtc ggacagtccg tggcggcgaa cgaagccatt ctcaagctcg
    11941 tgcccacagg cgagggcatc gtggtcaacg cgctcgtcgc gaacgcggat atcggtcgaa
    12001 tcgcgatcgg catgccagcg acgatcaagg tccgtgccta cgagttcgcg cggttcggcg
    12061 ccatcgaggg gacggtcgag cagatcagcg cggatgccat cgccgccgaa acgcccggcg
    12121 agccgccgag cttcgcggtg cggatcgcca cggaccagga ccagctggcg cccgacggca
    12181 gcctccccgt gctgcccggc atggcggtcg acgtcgagat ccgcatcggc gagcggacga
    12241 tcctctccta tttcacggac agcctgacgc gcgtgaagga gcgcgcgttc aaggacggtt
    12301 gaggtctggc gcgctcaggg agtgcggccg cccacgccga aattcttcct gatgtcgcgc
    12361 tgtgggccgg acatctcgag cacctctgcc gcacctccct cggggccgac cgaaatcgtg
    12421 aaggtgcggg tggcaaagcc gacgcagctc tgcggctgga tgaagccgag cgcgtcgacc
    12481 tcgacggagc tgaaggaccg gtcctggtag gtatagcgca accgcaggac gaggcgctcg
    12541 tcggtctcct cgagaatctc cgtctgggtg atcgctcgca tgaggggcgt cnnnnnnnnn
    12601 ngcgtcgggc agaggccgcc ttcctccgtg gcatgatcgg cgtaatagtt cttgaccggc
    12661 acgacgaggc cctcgcgccc ggccagcgag ttcatgccgg cgcagcccgc caggaccaga
    12721 agccccatgc ccaatcctac cgctggacgc attggcgaat tccccttcag gtgccgacgc
    12781 tgccggccat tgccggcagc gtcagggtca tgaccgtacc ggaatcgtcg ctttttcgca
    12841 cctgcagatc gccgccgtgc gcccgggcga tctcgcggca gatggcgagg cccaatccgg
    12901 cgccattgcc ggagctcgag gtggtgaagg gctcgaagag atgatcgcgc acggtctccg
    12961 gcaggccatg cccggtatcc tcgacctcga ccacaaaccg gtcggcgacg cggcggttcc
    13021 ggaacgtcag ctgcccgcca ccgggcatcg cttccagggc gttccgcgcg aggttgagga
    13081 agaggcggtg gagctggtcc ggatccgcca cgaccatgac gtcgggggcg atctcgttga
    13141 cgatggtggt gggcgtcccg gccggcgaca ggagggtcgt cacttcgtcg accagcgcgc
    13201 gcagcgccac cggcgcggtg cttggccggg gcgggcgact gcgggcgaag tcaagggttt
    13261 cctggcaaag gcggatagcc cgctcgagcg tctgcacgag cctcggcgtc accgctcgca
    13321 ccgctggatc ggcgctgccc tcaaggcggt cggagagcag gaaggcggtc gccaggatgt
    13381 tgcgcaggtc gtggtgcagc ttgccgaccg cctcgccgag ggccgccagc ctggtcttct
    13441 gggccagggc ctggcgcagg tcgcgggtca tcgcatcgac ctcgcgatcg acgatgccga
    13501 tctcgtcgct gcgtcgtgat gccggcgtat cgagcgagga gtcctcggga cgtttgcgga
    13561 aatgggcgat cttcgcggtg atccgggcga gcggccggac gatgagcgag cgcaggctgg
    13621 cgagcagcag gagcccgacg atcagcgaga gcgcgaagga caggaggatg atgcgccggg
    13681 catagtcgat catctcggcg tggagcatcg cctcggtcag ggtgatgtcg acggtggtgc
    13741 cgtattccat gggtgatggc ccgaccaccc gcaccacccg ctgaccgcca tgataaaggg
    13801 tcgagaaggc ctcgaggatc agtccgaccc agtcccggtc ggtgatgtcg atcgtccgct
    13861 cgaccggcgg cagcatgccg agcatcagca ccggccgctc gcgctcgatc gtgatcgaga
    13921 gcaccccggc atggctgagc agggcatcgg cgagcgccag ggaagggggc tggccgcggg
    13981 cggcgacgcc gatggtcgcg agatgcgccg cggcgatgcg ctcctcgaga tagacgtgcc
    14041 ggaagcgggc gatcgacggc acgaagatca ggatctcgcc gagcagcagg aagcccaccg
    14101 tcagcagcag caggcgcgcc gagaggctcc gaccccatgg cgggcgatct cctggcggcc
    14161 tcatgaccgg cccgaaccgc agcgcccgcg cgggcggtca gggcgcaagt gccaggataa
    14221 gccccggctg gggcggcgga ggttcaagag gcctcgatcg accgtgtcgt tggggcatca
    14281 gtcataccgg cagatgtcga tcgcctcaac gtcggccagc atttcgcgcc ggtggcgcgg
    14341 gtgcggacga cttcctgcat ttctcgtcgt ttgacttgca acccggctca ccctatgtgt
    14401 gcgccgcggt cggggttcgc cggcccgcac gtcctgtctt tttgccgaat aacgaggaat
    14461 tgaccgtgaa aagaacgtac cagccgagcc ggctcgtgcg caaaaggcgc cacggctttc
    14521 gggcgcggat ggccactgtg ggcggccggc gggtcattcg cgaccgccgg gccaagggcc
    14581 gcaagcggct gagtgcctga gccagaccgc cggttccggt gatcgcctgc tccgtcggct
    14641 cgaaaagcgt cgggacttcc tcagggtggc gcgaggtcgc aaggccgtgc gccccgcatt
    14701 cgtcgtccag atggcgccgg cacccgggcc agtatccgga gacgtggacg atggcgacgt
    14761 cgcgacggaa gcctggcgga tcggcttcac ggcctcgcgc aaggtcggtg gtgccgtgca
    14821 gcgcaaccgc gccaagcggc ggctgcgggc ggttgcccgc gcggtcatgc cggagctggc
    14881 gcatcgcggg ttcgattatg ttctggtcgc ccggacgccg gtgctgacat gcccatacag
    14941 ccgactggaa gccgatctga cggcggctct gaaggaactg gcacgctggg cgcggccgtg
    15001 cagtggcgac gtcttgcgag ggcggtcgat ctgattgtcg cgagctttct aaggcttctg
    15061 gttcacgcct atcgctatct tctggcaccg gtcctgccgc gtggctgccg ctttgcgccg
    15121 agctgctcgc tctacgcgct cgaggcgctt gcccggcacg gtgcgatcac cggcggaggc
    15181 ctggcgctgc ggcgcctcgc ttcctgccat ccatggggcg gctccggcta tgatccggtc
    15241 cctgccgcag cgccgaggcg cggttgccgg tgtcccgaca gcctgcccgc agcaagtcag
    15301 gagccgatcg gtcagcaacg atgacagatc aacgcaatct cattctcgcg atcgtcattt
    15361 cggtcggcat catcctcggt ttccaggtgt tctacgagat gccgcggatg gagcggcagc
    15421 agcagctgac ggaaagccag gacacgggcc aggggagcga gggcgtggtc ccgccgtccc
    15481 cgacctcgtc cggcgccgcc tcgggtggcg cgccggccag cagcgccggc gtgggtgcgg
    15541 cgctggatcc caacgccggc ctcgcaacgt cgcgggccga ggccctggcg cgcctgccgc
    15601 gggtgcagat cgccaatggc cgcgtgcacg gctccctcgc cctcgacggc gtccggttcg
    15661 acgacgtcac gctggtcgac taccggcaga ccgtcgaccc gaattcgccc gagatcgagc
    15721 tgctcaaccc ggtcggcgcc agggacacct atttcgccga gttcggctgg gtgccggagg
    15781 gcgagatcgt ggtgccgaac gcggagacgc tctgggagca ggtcggcgac aagctgaccc
    15841 aggaggtgcc ggtcgagttc cgctgggaca acggccaggg cctgctcttc gccaagcagg
    15901 tggcggtcga cgagaacttc atgttcacgc tcgagcgctc ggtcaccaac acctccgatc
    15961 agccggtgac gctctttccc tatggcctcc tgagccgctg gggaacgccg aagacggcgg
    16021 gcttctacat tctccacgag gggccgatcg gcgtgctcga cggcacgctc aaggagatcg
    16081 actacgacga cctgcacgag gagccgatca ccctgagcag cacgggcggt tgggccggct
    16141 tcaccgacaa gtactggctg acctcgctcg tccccgacca ggccgaggag ctgcaggcga
    16201 acttccgcca cttcgtgcgc gacggccagg accgctacca ggtcgactat ctgggcacgg
    16261 cgcgggtcat ccagccgggg gccacgatca ccgtgaccga ccggctgttc gccggggcca
    16321 aggaggtccg gctgctcgat gcgtacagcg agacctacgg tatcccgctt ttcgaccggg
    16381 cggttgattt cggctggttc tacttcctga ccaagccctt cttctacatc ctgaccttct
    16441 tctaccacaa cgtcttcggc aattacgggc tcgccatcct ggctctgacc ctgctggtca
    16501 agatcgtctt cttcccgctg gccaacaagt cctaccggtc gatgagccag atgaagaagc
    16561 tgcagccgga aatggtcaag ctgcgcgagc ggcacgccaa cgacaagcag aagatgcagc
    16621 aggagctgat ggcgctctac aagcgcgaga aggtcaaccc gatggcgggc tgcctgccga
    16681 tcctgctgca gatccccgtc ttcttcgcgc tctacaaggt cctcttcgtc tccatcgaga
    16741 tgcggcatgc gccgttcttc ggctggatcc acgacctttc ggcgcccgac ccgacgagca
    16801 tcttcaatct cttcggcctc ctgcccttcg ccgtgccggc cttcctcatg atcggcgtct
    16861 ggccggtgct catgggcgtc accatgtggc tgcagttccg gctcaacccg cagccggccg
    16921 atccgatcca ggccaaggtg atggggctcc tgccgttcat cttcgtcttc ct
//
LOCUS       DAOTFI010000104        16665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4173_length_16665_cov_6.967249, whole genome shotgun sequence.
ACCESSION   DAOTFI010000104 DAOTFI010000000
VERSION     DAOTFI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_16665_cov_6.967249"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..598
                     /locus_tag="P5558_12040"
                     /pseudo
     CDS             85..598
                     /locus_tag="P5558_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330183.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(920..995)
                     /locus_tag="P5558_12045"
     tRNA            complement(920..995)
                     /locus_tag="P5558_12045"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(960..962),aa:Phe,seq:gaa)
     gene            complement(1042..2598)
                     /locus_tag="P5558_12050"
     CDS             complement(1042..2598)
                     /locus_tag="P5558_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRY25087.1"
                     /translation="MALRFDRCTIDPDAFELRRDGEVVPVEPQVLELLLFLATNPGRL
                     VTRDELVEAVWRGRIVSEATIASRIKAARRAIGDDGTAQRLIATVHGRGFRFLAGVRD
                     DADGTPASPAAPPIAIEVPTDRPSLAVLPFRYLADDPAYRFLLAGLQDEVTAGLGRVR
                     SYLVVNSGSAASAAAVSDDLRAIGAGLGVRYLVTGSLRAGAGRLRVTAHLVEAASGIE
                     VWSGRFESEAGDTFELQDAITEALIGALSPALYAAEADRLKRQRPAVLAAYDRVLKAI
                     PDCWAVERAASRRAMAELEAAIAADPDYGLAHALLSWCHGQQVVYNWTSRPADHRATA
                     LTLARRGYALDPTDSMVLTLLATAESAAGEVLAARRHLDRGLEIDSNCCWAWNRSGYL
                     HCYTGDPDAGIADFERSLRLSPLDPLRHLSFVGMGLAGFVAADYPSALGWIDRALADR
                     PGILWVNRLVAACAALAGDMERAARAVAIVQGYAPGIRADDIVRAIPHQVEATRERYR
                     RALVLAGFRP"
     gene            2743..3333
                     /locus_tag="P5558_12055"
     CDS             2743..3333
                     /locus_tag="P5558_12055"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRY25088.1"
                     /translation="MESKTMPTMVNGLDQDALFDTLEAVKKDPKIAKFVFRAKNHWQT
                     GGVNETVIKDYYGVGEEHCRPGGFHLVNDEPPELLGTDKAPNPVEYVLHAMAGCITTS
                     LIAHATARGIRIDEVATQFSGDLDVQGFLGLDPKVRNGYQAVKVELFVKGDADAATLE
                     ALGHFARSRSPVFDIVTNGVPVSLEVTAEPSAAAAA"
     gene            complement(3389..4030)
                     /locus_tag="P5558_12060"
     CDS             complement(3389..4030)
                     /locus_tag="P5558_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25089.1"
                     /translation="MPADARSELDEARRSLGFLTVREVVERIAGRNIVLDPGSLLIGR
                     DARIGQGNVFHAAVEIGADARSTVTIGDGNQFFSTCMLRARAGGRIEIGQGNQFGEGG
                     LVLDADQAGAPITIGDHGRYRYGAVIRGRASLGSGSQVLGPVIVEDCELGAGASFAEP
                     DPDLRGGVLKGAGPARHLRVPQGHVIAAWGPFAQAAMQQQSFFHPTPEVDAGA"
     gene            complement(4030..4719)
                     /locus_tag="P5558_12065"
     CDS             complement(4030..4719)
                     /locus_tag="P5558_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRY25090.1"
                     /translation="MSGVIDWQRVAGDLDANGWADLGPLVTDAEMRELEALYPEDRPW
                     RSHVRMQRHGFGQGEYKYFADPLPEPVQRLRAGLYPPLAAIADRWEERLGSARRFPPD
                     HAALRELCRSHGQTRPTPLVLKYVAGDYNCLHQDVYGPACFPLQVVILLSDPATFTGG
                     ELVLVEQRPRRQSRAEVVPMARGRGVVFAVDQRPRQGSRGAHRVRQRHGVSRIHRGER
                     MTLGIIFHDAA"
     gene            complement(4716..5789)
                     /gene="ada"
                     /locus_tag="P5558_12070"
     CDS             complement(4716..5789)
                     /gene="ada"
                     /locus_tag="P5558_12070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HRY25091.1"
                     /translation="MLTAPADAPATGPGWKWRAVAERDAEADGAFVFAVRTTGVYCRP
                     SCPSRRARPENILFFDLAREAEAAGFRPCKRCRPTELSLQQARTLAIEAACRTIEAAE
                     TPPSLATLAEAAGLSPYHFHRTFRAVTGVTPKAYAGQVRERRATATLRDSGGVTRAIY
                     DAGYDSASRFYDGAKERLGMAPRTFRRGGEGEVIRSAIAESSLGLVLVAATERGICAV
                     RFGEDARALEAELHALFPRARHLPADPAFSELARSVIGRIEQPEAALELPLDIQGTLF
                     EQRVWAALRAIPTGETRSYGAVAREIGQPTAARAVARACAANPVAVLTPCHRVVPAAG
                     GLGGYRWGSARKGELLRREGAGK"
     gene            complement(5887..8019)
                     /gene="glgX"
                     /locus_tag="P5558_12075"
     CDS             complement(5887..8019)
                     /gene="glgX"
                     /locus_tag="P5558_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRY25092.1"
                     /translation="MSENEYRVWPGLPYPLGATWDGSGTNFTLFSAHAEKVELCLFDD
                     DGKRELARVALPEFTHEIWHGYLPDVRPGQLYGYRVHGPYDPAAGHRFNHHKLLLDPY
                     AKAAHGTIEWDDAVYGYTIGHPKADLSFDTRDSAPFMPKCMVIDPAFTWGRDRLTQRP
                     WHETIIYEMHVKGFTMRHPEVAEELRGTFGGLSSAPVVEYLRDLGVTAVELLPVHAFA
                     NDRHLIERGLNNYWGYNTLGFFTPHPAYLGKGGIGEVKTFVHVMHDAGIEVILDVVYN
                     HTAEGNHLGPTLSFRGIDNRSYYYLTEDPRYYNDFTGTGNALELRHPYVLRMVTDSLR
                     YWVEEMRVDGFRFDLASTLARVEGSYDEHASFLDVVAQDPALTKAKMIAEPWDTGMGG
                     YQVGNFPPGWAEWNDQYRDTVRKFWKGDAGQLPDLASRLSGSADIFDHRGRQPWASIN
                     FITAHDGFTLRDLVSYDQKHNEANGEDNRDGTDNNNSWNCGVEGPTDDPGIQALRRRQ
                     MRNFLTTLLLSQGVPMLVAGDEFGRTQGGNNNAYCHDNEIGWIDWNLDEDGQSLLAFT
                     RRLIHLRRDHIVFHRARFFHSALIPGTEVKDVTWLRADGGEMAEGDWTNGGRKMLTIL
                     LSGEAGLTHLTERGEKETDDTFLLILNASHEPADQHLPAAGDGLHWQVLIDTARDDDA
                     VDEELFAPGYEIGVDARTAMLLVARPAE"
     gene            complement(8111..8632)
                     /locus_tag="P5558_12080"
     CDS             complement(8111..8632)
                     /locus_tag="P5558_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRY25093.1"
                     /translation="MITAPAKAVLQTLFLAFFGIEQDPTIDRTPYEQELACLAQTVWF
                     EARGSNFADKLAVAQVVVNRVASREHADTVCDVVWEPAQFSWTADGLSDKVVIEGPID
                     ENAWTDSALAALTAYSAALPDLTAGATFYHADYVSPTWADQLHARGRFGAHLYYAAFE
                     PAPEIEPPVPAPR"
     gene            complement(8860..10614)
                     /locus_tag="P5558_12085"
     CDS             complement(8860..10614)
                     /locus_tag="P5558_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25094.1"
                     /translation="MRIRSKRLLCGVAALALLAQSGPAVADMAAAERWVDEEFQPSTL
                     TREEQLAEMAFFIEAAQRLIDQGITEINVVSETIDTHVYESQVLAPAFTEITGIKVNH
                     DLIQEGDVIEKIQTQMQSGENIYDAYVNDSDLIGTHSRYGFVVPLSDWMAGDGAEYTL
                     PSLDLDDFMGLSFTTGPDGKLYQLPDQQFANLYWFRYDWFTDPDIKAKFKEAYGYDLG
                     VPVNWSAYEDIADFFTNKVKEIDGVRVYGHMDYGKKDPSLGWRFTDAWLSMAGTGDKG
                     IPNGVPVDEWGIRMEGCNPVGSSVSRGGAANAPSAVYALTKYIDWLKAYAPPEAPGMT
                     FSEAGPVPGQGNIAQQIFWYTAFTAALADPSLPVMNEDGTPKWRMAPSPHGPYWEEGM
                     KLGYQDVGSWTLLKSTPVERAQAAWLYAQFTTAKTTSLKKTQVGLTPIRNSDINDESF
                     TERAPKLGGLVEFYRSPARVAWTPTGINVPDYPRLAQLWWANVAEAVTGEKTPQEAMD
                     SLAQQQDQVMARLERANVQGECGPKLNPEQDPEYWLSQPGAPKPKLENEKPQGVTVDY
                     DLLLQAWVDGKVMPDGVK"
     gene            complement(10711..10992)
                     /locus_tag="P5558_12090"
     CDS             complement(10711..10992)
                     /locus_tag="P5558_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HRY25095.1"
                     /translation="MPALEWMNWTLPTAAFFAAVGVMLLFMTALELAWPTVERKGLLP
                     MATTRGDRLFIGLLGAAFIHLGWLGFTELPLWYGSIAAFVWFTLEMRWG"
     gene            complement(11005..11802)
                     /locus_tag="P5558_12095"
     CDS             complement(11005..11802)
                     /locus_tag="P5558_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25096.1"
                     /translation="MRLRYLALIVYFGFLLLPIYWLVNMSLKTNVEITSGLTLWPQNL
                     TFANYVTILTDQSWYSGYINSFIYVAMNTAISLTVALPAAYAFSRYRFIGDKHMFFWL
                     LTNRMAPPAVFVLPFFQLYQTFGLFDTHIAVALAHCLFNVPLAVWILEGFMSGVPKEI
                     DETAYVDGYSFPRFFLFVFMPLIKAGIGVTAFFCFMFSWVELLMARTLTSVAAKPIGA
                     IMTRTVSAAGMDWGTLAAAGVLTIVPGVLVIYFVRNYMAKGFALGRV"
     gene            complement(11799..12674)
                     /locus_tag="P5558_12100"
     CDS             complement(11799..12674)
                     /locus_tag="P5558_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25097.1"
                     /translation="MRKTESNKAWLLVLPVFLLVAFSAIIPLMTVVNYSVQDIFGPGE
                     AYWVGTEWFKEMLRSEDLRGAFVRQVIFSATILVIQIPLGIAIALAMPAKGWAASLSL
                     VLVALPLLIPWNVVGTIWQIFARGDIGLGGWFVNDMMGIGYNYTADSLHAWITVVVMD
                     IWHWTPLVVLLCYAGLRAIPDAFYQAAAIDGAGRWKVFRYIQLPKLRGVLLIAVLLRF
                     MDSFMIYTEPFVLTGGGPGTATTFLSQQLVKIALGQFDLGPAAAYSIIYFLIILAASF
                     VFYNIMVRVGRGDRA"
     gene            complement(12698..13786)
                     /locus_tag="P5558_12105"
     CDS             complement(12698..13786)
                     /locus_tag="P5558_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25098.1"
                     /translation="MARIEFQRLAHSYLADPVAPADWALKELDFAWEDGGAYALLGPS
                     GCGKSTLLNIISGLLRPSQGRVLYDGKDVTGLSPAARNIAQVFQFPVIYDTMTVAENL
                     AFPLRNRRMPRAEVRKRVAEALEMLELQPVQNRRAAGLTADQKQKISMGRGLVRDDVA
                     AILFDEPLTVIDPHLKWQLRRKLKEVHERFHHTLVYVTHDQNEALTFADKVVVMYEGE
                     VVQLGTPQELFERPAHRFVGYFIGSPGMNFMGCHLVEDGLGIDGTELVLYGHWAELAR
                     RHAKSRLAIGIRPEFLDISPTPRDDAVPTRLMGVQDLGNFKLVTVRLGPHEVVAKLPE
                     DAPVPTDEAHLVFQPEWTRLYADDRLVG"
     gene            complement(13800..14900)
                     /locus_tag="P5558_12110"
     CDS             complement(13800..14900)
                     /locus_tag="P5558_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25099.1"
                     /translation="MTTLELRSVNRLEGDELHLDDIRLSLESGRLYVLLGPTNAGKTS
                     LLRVMAGLDRPSTGRVLIGGQDVTGRSVRRRNVAMVYQQFVNYPSFTVYDNIASPLRL
                     KGLRRAEVDKKVRETAELLHIEPLLQRLPAELSGGQQQRCAIARALVKEAELLLLDEP
                     LVNLDYKLREELRTELRALFERRSAIVVYATTEPMEALVMGGHAVVMGEGRVLQSGPT
                     LEVYHRPSSARVAAVFSDPTMNMVDVNIRGGEARTPAGLVLPLGGELADLRQGRFRLG
                     VRANHLSLRRRGAADIEIKGTVDLAEISGSETFVHFRHNNLDWVVQEAGVHPLARGAE
                     ASFYLDPAKLYFFGDTGALERAPSSLPLDSAA"
     gene            complement(14897..15898)
                     /locus_tag="P5558_12115"
     CDS             complement(14897..15898)
                     /locus_tag="P5558_12115"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRY25100.1"
                     /translation="MTAASAPTLAIVGAGTWGTALAQAAARAGREVLLWDRDPAIVRA
                     ITEDRRSPRAFPDLELEPGIRAEGDLATVLACEVVLLVVPAQAIRGLCRSVPGARPGV
                     VVVCAKGFESTTDELLSAVVEAEWPGTAVACLSGPSFAAEVASGLPTALTLGAVEPEL
                     GARLAELLIGPHLRVYWTDDRAGVEIGGAVKNVLAIASGMVTGAGLGENARAALVTRG
                     LAELARLGAAMGARAETLMGLAGLGDLMLTAMSATSRNTAFGMALGQGRSVAEAGAGG
                     ALVEGFWTARAVDRLATRHGVETPIARAVARILDGRLGIDEALATLMHRPLRAEGLA"
     gene            complement(15895..>16665)
                     /locus_tag="P5558_12120"
     CDS             complement(15895..>16665)
                     /locus_tag="P5558_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY25101.1"
                     /translation="PYILQNTDKRIVFVIPYENEYSLIGTTDVDYEGNPAEAAITPAE
                     TDYLCAVVNRYFRRTIGPADVVWSYAGVRPLYDDASGSASAVTRDYVFDVEGGAGRAP
                     LLSVFGGKITTYRKLAEHALQRLCPLLDCPKGDWTAGAPLPGGDLPEGDFTAFLARFR
                     GDHPWLPAALATRLARSYGSRATAIIEGRRGLDDLGEHLGGDLYAAELDYLVAHEWAM
                     APEDVLWRRSKLGLHLDEAARRRVADWFDRMPAKLSVA"
BASE COUNT         2639 a         5381 c         6155 g         2490 t
ORIGIN      
        1 tagaggactt ccacctccaa gctgtcgaac atgctcggca cacagatcgg atcgatcgcc
       61 tgctgacgat cgatgatctt caccgcaagc tggcagcgct gccgggtgca cttcatgcgc
      121 aacgccctgg cccacgccgg caagagcggt cgccgcgtcg tctctgcctt catcgccacg
      181 gcgtttgcgc aaaatgatgc tcaagccgcc agtcgacagt ggcgccgcat cgccgaccaa
      241 ttgcgcccaa acatcgatcg cctggcaagg ctcatggacg aggccgagcc cgacgtcctc
      301 gcttacatga ccttcccgca gcagcacagc accaagctgc acagcgccaa tcccatcgag
      361 cgcctcaacg gcgagatcaa gcgtcgcacc gaagtcgtcg gcatcttccc caacgaagcc
      421 gccatcaccc ggggccccca tgcggcctgc cgcatggggt ggcgcccgcc tggtcggtgc
      481 cctgctcctc gagcaaaacg acgagtgggc cgtccagcgc gcccgctaca tgacccttga
      541 gagcatcgcc cacaccggcg atgataccac cgtcagcctg accaacatga caggttgaac
      601 agcaaccggt ccaatccgga gaactggccg gccaaaggtc agctacacca cgtcccggga
      661 catgaccgag tgtgacgagc ggtcgcaatt tctcttcgat gattgcctgg aacgaacacc
      721 gccgacgcca agccgaccgg tggaccactg acaccgcagg tcaatgaagg ctgactctgc
      781 cacaccgatg caggtccgcc caaaatatcc aaggaaagca gatctctcag ctagcgttgc
      841 accagatcgc cgttaacgtc tttggtgcca aaacggtgcc aaacattatc ggggaaatca
      901 gttaagtcat tgaaataaat ggtgcccagg gacggatttg aaccgccgac acacggattt
      961 tcagtccgtt gctctaccaa ctgagctacc tgggcgcagg cgcggatata aggggtcggt
     1021 cgtggcctgt ccagccgtgc gtcaggggcg aaagccggcg aggacgaggg cccggcggta
     1081 gcgctcgcgg gtcgcctcga cctggtgcgg gatggcccgg acgatgtcgt cggcgcggat
     1141 gccgggcgcg tagccctgga cgatggcgac ggcgcgggcg gcgcgctcca tgtcgccggc
     1201 gagggcggca caggcggcga ccaggcggtt gacccagagg atgcccggcc ggtcggcgag
     1261 ggcgcggtcg atccacccga gggcggacgg gtagtcggcg gcgacgaagc cggcgaggcc
     1321 catgccgacg aagctcaggt ggcgcagcgg gtcgaggggg ctgaggcgga gcgagcgctc
     1381 gaagtcggcg atgccggcat cgggatcgcc ggtgtagcag tgaagatagc ccgagcggtt
     1441 ccaggcccag cagcaattgg agtcgatctc gaggccgcgg tcgagatggc ggcgggcggc
     1501 caggacctcg cccgcagcgg attcggcggt ggcgaggagg gtcaggacca tggaatcggt
     1561 cgggtcgagc gcgtagccgc ggcgggcgag ggtcagtgct gtagcgcggt gatcggcggg
     1621 gcgggaggtc cagttgtaga cgacctgctg gccgtggcac caggagagca gggcatgggc
     1681 gaggccatag tcggggtcgg ccgcgattgc ggcctcgagc tcggccatgg cgcggcggct
     1741 ggcggcgcgc tccaccgccc agcagtcggg aatggctttc agcacgcggt cgtaggcggc
     1801 gagcacggcc ggccgctggc gcttcaggcg gtccgcctcg gcggcgtaga gggccggtga
     1861 gagggcaccg atgagggcct cggtgatggc gtcctgcagc tcgaacgtgt cgcccgcctc
     1921 gctctcgaag cggccggacc agacctcgat gccgctcgcc gcctcgacca gatgcgcggt
     1981 gacgcgcagc cgcccggcgc cggcccgcag gctgccggtg acgaggtagc gcacgccgag
     2041 cccggcgccg atggcccgca gatcgtcgct cactgcggcg gccgaggcgg cggagccgct
     2101 gttgacgacg agatagctgc gaacgcggcc gagcccggcg gtcacctcgt cctgcagccc
     2161 ggcgaggagg aagcggtagg cggggtcgtc ggccaggtag cggaacggca ggacggcgag
     2221 gctcggccgg tcggtcggca cctcgatggc gatcggcggg gcggcggggg atgccggggt
     2281 tccatcggcg tcgtcccgga ccccggcgag gaagcggaag ccgcggccat gcacggtggc
     2341 gatcaggcgc tgggcggtgc cgtcgtcgcc gatggcgcgg cgggccgcct tgatgcggct
     2401 ggcgatggtc gcctcggaga cgatgcggcc ccgccagacg gcctcgacca gctcgtcccg
     2461 ggtgaccagc cggccgggat tcgtggcgag gaagaggagc agctccagga cctggggctc
     2521 gaccggcacg acctcgccgt cccggcgcag ctcgaacgcg tcggggtcga tcgtgcagcg
     2581 gtcgaagcgc agcgccatgc caccctcccc ggcgccgatt atcgaggaaa tccgcaagct
     2641 ctccaagaat tctccaggcc ggcttcaagc accgcctcgg gccttcgcct attcaggaga
     2701 ggcgccggca atgccgccgg cggaagctcg agggagacga tcatggagag caagacgatg
     2761 ccgacgatgg tgaacgggct ggaccaggac gccctcttcg acacgctcga ggccgtgaag
     2821 aaggacccaa agatcgcgaa gttcgtgttc cgcgccaaga accactggca gacgggcggg
     2881 gtcaacgaga cggtgatcaa ggactattac ggtgtcggcg aggagcattg ccggccaggc
     2941 ggcttccatc tggtcaacga cgagccgccg gagctgctcg gcaccgacaa ggcgcccaac
     3001 ccggtcgagt acgtgctgca cgccatggcg ggctgcatca ccacctcgct catcgcccac
     3061 gccaccgcgc gcggcatcag gatcgacgag gtggcgacgc agttctcggg cgatctcgac
     3121 gtgcagggct tcctcggcct cgatccgaag gtcaggaacg gctaccaggc ggtgaaggtg
     3181 gagctcttcg tcaagggcga cgcggacgcg gcgacgctcg aagcgctcgg ccacttcgcc
     3241 aggtcgcgct cgccggtctt cgacatcgtc accaacggcg tgccggtcag cctcgaggtg
     3301 acggccgagc cgtccgccgc cgccgcggcg tgagcccgcc gctcgggcat gggggccgct
     3361 ccggcgacgg ggcggccccg ttgctgcttc atgcgccggc gtcgacctcc ggcgtcgggt
     3421 ggaagaagct ctgctgctgc atggccgcct gcgcgaacgg gccccaggcg gcgatgacgt
     3481 ggccttgcgg cacacggaga tgccgggcgg ggccagcgcc cttgaggacg ccgccgcgaa
     3541 ggtcgggatc gggctcggcg aaggaggcgc cggcaccgag ctcgcaatcc tcgacgatca
     3601 ccgggcccag gacctggctg cccgagccga gcgacgcccg gccgcggatg acggcgccgt
     3661 agcggtagcg gccgtggtcg ccgatggtga tcggggcacc cgcctggtcg gcatcgagga
     3721 cgaggccgcc ctcgccgaac tggttgcctt ggccaatctc gatccgcccg cccgctcgcg
     3781 cacggagcat gcaggtcgag aagaactggt tgccgtcgcc gatggtcacg gtgctccgtg
     3841 cgtcggcgcc gatctcgacg gcggcgtgga acacgttgcc ctggccgatc cgcgcgtcgc
     3901 ggccgatcaa cagcgagccg ggatcgagca cgatgttgcg accggcgatg cgctccacga
     3961 cctcacgcac cgtgaggaag cccagggacc ggcgtgcttc atccagctcg gaacgggcgt
     4021 cggccggcat caggcggcgt cgtggaagat gatgcccagc gtcatgcgct cgcctctgtg
     4081 gatgcggctg acgccgtggc gctggcgcac gcggtgagcg ccccggctgc cctggcgcgg
     4141 gcgctggtcg acggcgaaga cgacgccgcg gccgcgggcc atgggcacca cctcggcgcg
     4201 cgactgccga cgcgggcgct gctcgaccag caccagctcg ccgccggtga aggtggcggg
     4261 atcggagagg agaatgacga cctgcagcgg gaagcaggcg gggccataga cgtcctgatg
     4321 gaggcagttg tagtcgccgg cgacgtactt gagcacgagc ggggtcggcc tggtctggcc
     4381 atggctgcgg cagagttcgc ggagcgcggc gtggtcgggc gggaagcgcc gggcggaacc
     4441 gaggcgctcc tcccagcggt cggcgatggc agccagcggc gggtagaggc cggcgcgcag
     4501 gcgctggacc ggctcgggca aggggtcggc gaaatacttg tactcgccct ggccgaagcc
     4561 gtggcgctgc atgcggacat ggctgcgcca gggccggtcc tcggggtaga gggcttcgag
     4621 ctcccgcatc tcggcgtcgg tgacgagcgg gccgagatcg gcccagccat tcgcgtcgag
     4681 gtcacccgcg acacgctgcc agtcgatcac gccgctcact tgccggctcc ctcgcggcgc
     4741 aggagctctc ccttgcgcgc gctgccccag cgatagccgc cgagcccgcc ggcggccggc
     4801 accacgcggt ggcagggggt gaggacggcg accgggttgg cagcacaggc gcgggcgaca
     4861 gcacgggcgg cggtcggctg gccgatctcc ctggcgacgg cgccatagct gcgggtctcg
     4921 ccggtgggga tggcgcggag cgccgcccag acgcgctgct cgaagagcgt gccctggatg
     4981 tcgagcggca gctcgagggc agcttccggc tgctcgatcc ggccgatcac cgaccgggcc
     5041 agctcgctga aggccggatc ggccgggagg tggcgggctc tcgggaagag ggcgtgaagc
     5101 tcggcttcga gcgcccgcgc gtcctcgccg aagcggacgg cgcagatgcc gcgctcggtc
     5161 gccgccacga ggacgaggcc gaggctgctc tcggcgatgg cggagcggat cacctcgccc
     5221 tcgccacccc ggcggaaggt cctgggtgcc atgccgagcc gctccttcgc cccgtcgtag
     5281 aagcgcgagg ccgaatcata gccggcatcg tagatcgccc gggtgacgcc gccgctgtcg
     5341 cgcagcgtgg ccgtggcgcg gcgctcgcgg acctgcccgg cataggcctt gggcgtgacg
     5401 ccggtgaccg cgcggaaggt ccggtggaag tgataggggc tgagcccggc cgcctcggcg
     5461 agggttgcga gcgacggcgg ggtctcggcc gcctcgatcg tccggcaggc cgcctcgatg
     5521 gccagcgtcc gcgcctgctg aagcgagagc tcggtcggcc ggcagcgctt gcagggtcga
     5581 aagccggcgg cctcggcctc gcgggcgaga tcgaagaaga ggatgttctc gggcctggcc
     5641 cggcggctcg ggcagctcgg ccggcaatag acgccggtcg tccggacggc gaagacgaag
     5701 gcgccgtcgg cctcggcatc gcgctcggcg acggcccgcc atttccagcc ggggccggtg
     5761 gcgggtgcat cggcgggggc ggtgagcatg ggcttctcct ccaaacgttg cgccgttggc
     5821 gcgtcgggtc cgggagaatg gcgctttcgc acgggtcgcc ccatccgatt cttgcggccg
     5881 aaaagcctat tcggccggcc gggccacgag gagcatggcg gtgcgggcgt cgacgccgat
     5941 ctcgtagccc ggggcgaaga gctcttcgtc gacggcgtcg tcgtcccgcg cggtgtcgat
     6001 caggacctgc cagtgcaggc cgtcgcctgc cgccggcaga tgctggtcgg ccggctcgtg
     6061 cgaggcgttg aggatcagga ggaaggtgtc gtcggtctcc ttctcgccac gctcggtcag
     6121 gtgcgtcagc ccggcctcgc cgctgagcag gatggtgagc atcttgcggc cgccattggt
     6181 ccagtcgccc tccgccatct cgccgccatc ggcgcgcagc caggtgacgt ccttgacctc
     6241 ggtgccgggg atgagggcgc tgtggaagaa ccgtgcgcgg tggaagacga tgtggtcgcg
     6301 gcgcaggtgg atcagccggc gggtgaacgc cagcaggctc tggccgtcct cgtccaggtt
     6361 ccagtcgatc cagccgatct cgttgtcgtg gcagtaggcg ttgttgttgc cgccctgggt
     6421 gcggccgaac tcgtcgccgg ccaccagcat cgggacaccc tgcgacagga ggagcgtggt
     6481 caggaagttg cgcatctggc ggcggcgcag cgcctggatc ccgggatcat cggtcgggcc
     6541 ctcgacgccg cagttccagc tgttgttgtt gtcggtgccg tcccggttgt cctccccgtt
     6601 ggcctcgttg tgcttctggt cgtagctgac gagatcgcgc agcgtgaagc cgtcatgggc
     6661 ggtgatgaag ttgatgctgg cccagggctg gcggcccctg tggtcgaaga tgtcggcgga
     6721 gccggagagg cgggaggcga ggtctggcaa ctggcccgcg tcgcccttcc agaacttgcg
     6781 cacggtgtcc ctgtactggt cgttccactc ggcccagccg ggcgggaagt tgccgacctg
     6841 gtagccgccc atcccggtat cccagggctc ggcgatcatc ttggccttgg tgagcgccgg
     6901 atcctgcgcc accacgtcga ggaagctcgc atgctcgtcg taggagcctt cgaccctggc
     6961 gagcgtcgag gcgaggtcga agcggaagcc gtcgacccgc atctcctcga cccagtagcg
     7021 gagcgagtcc gtcaccatgc gcagcacgta gggatggcgc agctcgaggg cgttgccggt
     7081 gccggtgaag tcgttgtagt agcgggggtc ctcggtcagg taatagtagg accggttgtc
     7141 gatgccgcgg aacgacaaag tggggccgag gtggttgccc tcggcggtgt ggttgtagac
     7201 gacgtcgagg atgacctcga tgccggcgtc gtgcatcacg tggacgaagg tcttgacctc
     7261 gccgatgccg cccttgccga gataggcggg atgcggggtg aagaatccca gcgtgttgta
     7321 gccccagtaa ttgttcaggc cacgctcgat caggtggcgg tcattggcga aggcgtgcac
     7381 cggcaggagc tcgaccgcgg tgacgccgag atcgcgcagg tactcgacga ccggcgccga
     7441 cgacaggccg ccgaaggtgc cgcgcagctc ctccgccacc tcggggtggc gcatggtgaa
     7501 gcccttgacg tgcatctcgt agatgatggt ctcgtgccag ggccgctggg tcagccggtc
     7561 gcggccccag gtgaaggccg gatcgatcac catgcatttc ggcatgaagg gggcgctgtc
     7621 gcgggtatcg aaggagaggt cggccttggg atggccgatg gtgtagccgt agaccgcgtc
     7681 gtcccactcg atcgtgccgt gggccgcctt ggcgtagggg tcgaggagca gcttgtggtg
     7741 attgaagcga tgcccggccg ccgggtcgta ggggccgtgg acgcggtagc cgtagagctg
     7801 gccggggcgg acgtcgggca ggtagccgtg ccagatctcg tgggtgaact cgggcaacgc
     7861 caccctcgcc agctcgcgct tgccgtcgtc gtcgaagagg cagagctcga ccttctcggc
     7921 atgggccgag aagagggtga agttggtgcc ggaaccgtcc caggtggctc caagaggata
     7981 gggcaggccc ggccagaccc gatattcgtt ttcgctcaat ctcaaaactc ccgctgcgat
     8041 cgacaacgcg catcgttcgc atgcgccggg agggcggttc cgtcaaggcg gcaaggcgcc
     8101 cgaaaggctg tcagcgcggc gccggcaccg gcggctcgat ctccggggcg ggctcgaagg
     8161 cggcatagta gagatgcgcg ccgaagcggc cgcgggcgtg gagctggtcg gcccaggtcg
     8221 ggctgacgta gtccgcatgg tagaaggtgg cgccggcagt gaggtccggc agggcggcgg
     8281 aataggcggt cagcgcggcg agggcgctgt ccgtccaggc gttctcgtcg atcgggccct
     8341 cgatcacgac cttgtcggag agcccgtccg cggtccagga gaactgggcc ggctcccaga
     8401 cgacatcgca gacggtgtcg gcatgctccc tcgaggcgac ccggttgacc acgacctggg
     8461 cgacggcgag cttgtcggcg aaattgctgc cgcgcgcctc gaaccagacg gtctgggcga
     8521 ggcaggcgag ctcctgctcg tagggcgtgc ggtcgatggt cgggtcctgc tcgatgccga
     8581 agaaggcgag gaacagggtc tgcagcacgg ctttggcggg ggcggtgatc atctcccggt
     8641 ctccgtctaa gcacccgatg caagtcagcg acgggcagcc tgatcgtccg ggatcaccgt
     8701 gccgtctcgt gctcctgctg ttttggtgaa catgaagcaa agcacagtat cagacgcctc
     8761 gccattaagg attcatcaac cctgttctcc gcctgagcaa cgcgaaacgg cgggggccct
     8821 ggcccccgcc gctccgtcat ccggcagtga atggacggct tacttgacgc cgtcgggcat
     8881 caccttgccg tcgacccagg cctgcagcag caggtcgtag tcgacggtca caccctgcgg
     8941 cttctcgttc tcgagcttgg gcttcggggc acccggctgg gagagccagt actcggggtc
     9001 ctgctcggga ttgagcttcg ggccgcactc gccctggacg ttggcgcgct cgagccgggc
     9061 catcacctgg tcctgctgct gcgcgaggct gtccatggcc tcctgcgggg tcttctcgcc
     9121 ggtcaccgcc tcggcgacat tggcccacca gagctgcgcc aggcgcgggt agtcgggcac
     9181 gttgatgccg gtcggcgtcc aggcgacgcg ggccgggctg cggtagaact cgacgaggcc
     9241 gccgagcttg ggggcgcgct cggtgaacga ctcgtcgttg atgtcgctgt tgcggatcgg
     9301 ggtgaggccg acctgggtct tcttcagcga ggtggtcttg gcggtggtga actgggcgta
     9361 gagccaggcc gcctgggcgc gctcgaccgg tgtcgacttc aagagcgtcc agctacccac
     9421 gtcctggtag ccgagcttca tgccctcttc ccagtagggg ccatgcgggg acggggccat
     9481 gcgccacttg ggcgtgccgt cctcgttcat caccggcagc gacgggtcgg ccagcgccgc
     9541 ggtgaaggcg gtgtaccaga agatctgctg ggcgatgttg ccctgccccg ggaccgggcc
     9601 ggcctcggag aaggtcatgc ccggcgcttc cggcggggca taggccttga gccagtcgat
     9661 atacttggtc agcgcgtaga cggccgacgg cgcattggcg gcgccgccgc ggctgacgct
     9721 ggagccgacc gggttgcagc cctccatgcg gatgccccat tcgtcgaccg ggacaccatt
     9781 ggggatgccc ttgtcgccgg tgccggccat cgagagccag gcatcggtga agcgccagcc
     9841 gagcgacggg tccttcttgc cgtagtccat gtggccatag acgcggacgc cgtcgatctc
     9901 cttgaccttg ttggtgaaga agtcggcgat gtcctcatag gccgaccagt tgaccggcac
     9961 gccgaggtcg tagccgtagg cctccttgaa cttggccttg atgtccgggt cggtgaacca
    10021 gtcgtagcgg aaccagtaga ggttcgcgaa ctgctggtcg gggagctggt agagcttgcc
    10081 gtccgggccg gtcgtgaagc tgaggcccat gaagtcgtcg aggtcgagcg acggcagggt
    10141 gtactccgcc ccgtcgccgg ccatccagtc gctgagcggc acgacgaagc cgtagcggct
    10201 gtgggtgccg atcaggtcgc tgtcgttgac ataggcgtcg tagatgttct cgcccgactg
    10261 catctgcgtc tggatcttct cgatcacgtc gccttcctgg atcaggtcgt gattgacctt
    10321 gatcccggtg atctcggtga aggccggggc cagcacctgc gattcgtaga catgcgtgtc
    10381 gatggtctcc gagacgacgt tgatctcggt gatgccctgg tcgatcagcc gctgggcggc
    10441 ctcgatgaag aacgccatct cggcgagctg ctcctcgcgc gtgagggtcg agggctggaa
    10501 ctcctcgtcg acccagcgct cggcggctgc catgtcggcg acggccggcc cgctctgggc
    10561 gagcagcgcc agtgcggcga ccccgcacag caagcgtttc gatcggattc tcatgaactg
    10621 cacctccctg gatgcgcccc cctcctcgac cgttgccggt cgccgcgacc gggggacctg
    10681 gtgtcgcgac agatgaaaat cggcagtgcg tcagccccag cgcatctcca gcgtgaacca
    10741 gacgaaggcc gcgatcgagc cgtaccagag cggcagctcg gtgaagccga gccagccgag
    10801 atggatgaag gcagcgccca ggaggccgat gaagaggcgg tcgcccctgg tcgtcgccat
    10861 gggcagcagg cccttgcgct cgacggtcgg ccaggcgagc tcgagcgcgg tcatgaagag
    10921 gagcatgacg ccgacggcgg cgaagaaggc cgccgtgggc agggtccagt tcatccactc
    10981 gagggcgggc atgccgatgt ctcctcagac ccgcccgagg gcgaagccct tggccatgta
    11041 gttgcggacg aagtagatga ccagcacgcc cggcacgatg gtcaggacac cggcggcggc
    11101 gagcgtgccc cagtccatcc ccgcggccga gacggtgcgg gtcatgatgg cgccgatggg
    11161 cttggccgcg accgaggtca gcgtgcgggc catcaagagc tcgacccagg agaacatgaa
    11221 gcagaagaag gcggtgacgc cgatccctgc cttgatcagc ggcatgaaga cgaagaggaa
    11281 gaagcgcggg aagctgtagc cgtcgacata ggcggtctcg tcgatctcct tcggcacgcc
    11341 ggacatgaag ccctcgagga tccagaccgc caagggcacg ttgaagaggc agtgcgccag
    11401 cgccacggcg atgtgcgtgt cgaagagccc gaaggtctga tagagctgga agaacggcag
    11461 gacgaagacg gccggcggcg ccatgcggtt ggtcagcagc cagaagaaca tgtgcttgtc
    11521 gccgatgaac ctgtagcgcg agaaggcgta ggccgccggc agcgccacgg tcagcgagat
    11581 ggcggtgttc atcgccacgt agatgaagct gttgatgtag cccgagtacc agctctggtc
    11641 ggtcaggatg gtgacgtagt tggcgaaggt caggttctgc ggccagagcg tcaggccgct
    11701 ggtgatctcg acattggtct tgaggctcat gttgacgagc cagtagatcg gcaggagcag
    11761 gaagccgaaa tagacgatga gcgcgaggta gcgcagcctc atgcgcggtc tccccggccg
    11821 acgcggacca tgatgttgta gaagacgaag ctggccgcca ggatgatcag gaaatagatg
    11881 atcgagtagg cggccgcggg cccgaggtcg aactggccga gggcgatctt gacgagctgc
    11941 tgcgagagga aggtggtggc cgtgccgggg ccgccgccgg tgagcacgaa gggctcggtg
    12001 tagatcatga agctgtccat gaagcggagc agcacggcga tcaaaaggac gcccctgagc
    12061 ttggggagct ggatgtagcg gaagaccttc cagcggccgg cgccgtcgat ggcggcggcc
    12121 tggtagaagg cgtcgggaat ggcgcgcagg ccggcgtagc agagcaggac gacgagcggc
    12181 gtccagtgcc agatgtccat gaccacgacg gtgatccagg cgtgcaggct gtcggcggtg
    12241 tagttgtagc cgatgcccat catgtcgttg acgaaccagc cgccgagacc gatgtcgccg
    12301 cgcgcgaaga tctgccagat cgtgccgacc acgttccagg gaatcagcag cggcagggcc
    12361 acgagcacga ggctcagcga cgccgcccag cccttggcgg gcatggcgag ggcgatggcg
    12421 atgcccaagg ggatctggat gacgaggatg gtggcggaga agatcacctg gcggacgaag
    12481 gcgccgcgca ggtcctccga gcgcagcatc tccttgaacc actcggtgcc gacccagtag
    12541 gcctcgccgg ggccgaagat gtcctggacg gagtagttga cgaccgtcat cagcgggatg
    12601 atggcgctga aggcgaccag caggaacacc ggcaggacca gcagccaggc cttgttgctc
    12661 tcggtcttgc gcatggtttc tccctgccct tcccgcctca gccgaccagc cggtcgtcgg
    12721 cgtagaggcg cgtccattcc ggctggaaca ccagatgcgc ctcgtcggtc ggcacgggtg
    12781 cgtcctccgg cagcttggca accacctcgt gcgggccgag gcggaccgtc accagcttga
    12841 agttgccgag gtcctgcacg cccatcagcc tggtcgggac ggcgtcgtca cggggtgtcg
    12901 gcgagatgtc gaggaactcg gggcggatgc cgatggcgag cctcgacttg gcgtggcggc
    12961 gggcgagctc cgcccagtgg ccgtagagca cgagctcggt gccgtcgatg ccgagcccgt
    13021 cctcgaccag gtggcagccc atgaagttca tgccgggcga gccgatgaaa tagccgacga
    13081 agcggtgcgc cgggcgctcg aacagctcct gcggcgtgcc gagctggacc acctcgccct
    13141 cgtacatgac caccaccttg tcggcgaagg tgagcgcctc gttctggtcg tgggtgacgt
    13201 agacgagggt gtggtggaag cgctcgtgca cctccttgag cttgcggcgg agctgccact
    13261 tgagatgcgg gtcgatgacg gtgagcggct cgtcgaagag gatggcggcg acgtcgtcgc
    13321 gcacgaggcc ccggcccatc gagatcttct gcttctggtc ggcggtgagc ccggcggcgc
    13381 ggcggttctg gaccggctgc agctcgagca tctcgagcgc ctcggcgaca cgctttctga
    13441 cctcggcccg cggcattcgg cggttgcgca ggggaaaggc caggttctcg gccaccgtca
    13501 tggtgtcgta gatgaccggg aactggaaca cctgggcgat gttgcgcgcc gccggcgaca
    13561 ggccggtgac gtccttgccg tcgtagagga cgcggccctg gctcggcctg agcaggccgg
    13621 agatgatgtt gagcagcgtg gacttgccgc agcccgaggg gccgagcagc gcgtaggcgc
    13681 ctccgtcctc ccaggcgaag tcgagctcct tcagcgccca gtcggccggc gccacgggat
    13741 cggcgaggta gctgtgggcg agacgctgga actcgatcct ggccacgcgc cgccgcccct
    13801 caggccgcgc tgtcgagcgg caggctcgag ggtgcccgct cgagggcacc cgtgtcgccg
    13861 aagaagtaga gcttagccgg gtcgagatag aagctcgcct cggcgccgcg ggcgagcgga
    13921 tggacgcccg cctcctgcac cacccagtcg agattgttgt ggcggaaatg gacgaaggtc
    13981 tccgagcccg agatctcggc gaggtcgacc gtgcccttga tctcgatatc ggccgcgccg
    14041 cgccgccgga gcgacaggtg gttggcgcgc acgcccaggc gaaagcggcc ctggcgcagg
    14101 tcggcgagct cgcccccgag gggcaggacg aggcccgccg gggtgcgggc ctcgccgccc
    14161 cggatattga cgtcgaccat gttcatggtg gggtcggaga agacggcagc gacccgggcc
    14221 gagctcggcc ggtggtagac ctcgagggtc ggccccgact gcagcacccg cccctcgccc
    14281 atgacgacgg catggccgcc catgacgagc gcctccatgg gctcggtcgt ggcatagacg
    14341 acgatcgccg agcgccgctc gaagagggcg cgaagctccg ttcgcagctc ctcgcgcagc
    14401 ttgtagtcga gattgacgag cggctcgtcg agcagcagca gctcggcctc cttgaccaaa
    14461 gcgcgggcga tggcgcaacg ctgctgctgg ccgcccgaga gctcggccgg caggcgctgc
    14521 agcaggggct cgatgtgcag gagctcggcc gtctccctga ccttcttgtc gacctcggcg
    14581 cgccgcaggc ccttgaggcg cagcggcgag gcgatgttgt cgtagacggt gaaggacggg
    14641 tagttgacga actgctggta gaccatggcg acgttgcgcc ggcgcacgct ccggccggtg
    14701 acgtcctggc cgccgatcag cacccggccg gtcgagggcc ggtcgagccc ggccatgacc
    14761 cggagcagcg aggtcttgcc ggcattggtg ggcccgagga ggacatagag gcgaccggat
    14821 tcgaggctca accggatgtc gtcgagatgc agctcgtcgc cctcgagccg gttcaccgag
    14881 cggagctcca gggtcgtcac gcgaggccct cggcgcgcag cggccggtgc atcagggtcg
    14941 ccagcgcctc gtcgatgccc agccggccgt cgaggatgcg ggcgacggcc cgggcaatcg
    15001 gcgtctcgac cccgtgccgg gtggcgagcc ggtcgacggc ccgggcggtc cagaagccct
    15061 cgacgagcgc gcccccggcc ccggcctcgg cgacgctgcg gccctggccg agcgccatgc
    15121 cgaaggcggt gttgcgcgag gtggcgctca tggcggtcag catcagatca ccgaggccgg
    15181 cgaggcccat cagggtctcg gccctggcgc ccatggcggc gccgagccgc gccagctcgg
    15241 cgaggccgcg ggtgacgagg gccgccctgg cgttctcgcc gagcccggca cccgtcacca
    15301 tgccggaggc gatggcgagc acgttcttca ctgccccgcc gatctccacg ccggcccggt
    15361 cgtccgtcca gtagacccgc agatgcggcc cgatcaggag ctcggccagc cgggcgccga
    15421 gctcgggctc gacggcaccc agcgtgagcg ccgtcggcag gccgctcgcc acctcggcgg
    15481 cgaagctcgg gccggagagg caggcgacgg cggtgcccgg ccattcggcc tcgacgacgg
    15541 cgctgagcag ctcgtcggtc gtgctctcga agcccttggc gcagaccacc accacacccg
    15601 gccgggcacc gggaaccgag cggcagaggc cgcggatggc ctgcgccggc acgaccagca
    15661 gcacgacctc gcaggcgagc acggtcgcga ggtcgccctc ggcgcgaatg ccgggctcca
    15721 gctcgaggtc gggaaaggcg cgcggcgagc gacggtcctc ggtgatcgcc cggacgatgg
    15781 ccggatcgcg gtcccagagc aggacctcac ggccggcccg ggcggctgcc tgagccaggg
    15841 ccgtccccca ggtcccggca ccgacgatgg cgagggtcgg ggcgctcgca gcggtcatgc
    15901 gacggagagc ttcgcgggca tgcggtcgaa ccagtcggcg acgcgccggc gggctgcctc
    15961 gtccaggtgc aggccgagct tgctgcggcg ccagagcacg tcctcgggag ccatcgccca
    16021 ctcgtgcgcc acgagatagt cgagctcggc agcgtagaga tcgccgccca gatgctcacc
    16081 gagatcgtcc agcccccgcc tcccctcgat gatggccgtc gcccgcgaac cataggagcg
    16141 cgccagacgg gtcgcaagcg cggccggcag ccatgggtgg tcgccccgga accgggcgag
    16201 gaaggccgtg aaatcgccct ccggcagatc gccgccgggc agcggtgctc ccgccgtcca
    16261 gtcgcccttc gggcagtcga gcagcgggca gagccgctgc agcgcgtgct cggcgagctt
    16321 ccggtaggtc gtgatcttgc cgccgaagac ggagagcagc ggcgcccggc cggcgccgcc
    16381 ctcgacgtcg aagacatagt cgcgggtcac cgccgaggcg ctgccgctgg cgtcgtcgta
    16441 gagcggccgc accccggcat aggaccagac cacgtcggcc gggccgatcg tgcggcggaa
    16501 gtagcggttg accacggcgc agagatagtc ggtctcggca ggcgtgatcg ccgcctcggc
    16561 cggattgccc tcgtagtcga catcggtggt gccgatcagg ctgtactcgt tctcgtaggg
    16621 gatgacgaag acgatgcgct tgtcggtgtt ctgcaggatg taggg
//
LOCUS       DAOTFI010000105        16645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4187_length_16645_cov_6.789030, whole genome shotgun sequence.
ACCESSION   DAOTFI010000105 DAOTFI010000000
VERSION     DAOTFI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 16645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16645
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4187_length_16645_cov_6.789030"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1252
                     /locus_tag="P5558_12125"
     CDS             53..1252
                     /locus_tag="P5558_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25102.1"
                     /translation="MTVEPRQPPESAGLRPAGNSEHWVDPEPWQVHREQPLTPEQERY
                     YTASQWRLMWWRFKRHKVAVASLWVLVLFYVVVVIAEVVAPYDAQTRDPLHIHAPPQP
                     IHLFHEGRFVGPFTYGYQMTLDMTTLKRIYEADRTMVQPLRFFCRGDAYRFWGQWPGD
                     FHLVCPAEGGTFFLLGTDRLGRDMFSRILYGARVSLTVGLVGIAISFALGIILGGVSG
                     YYGGWIDNIVQRLIEVIRSFPMIPLWLALSAALPPTWPPIWVYFGITLILGLVDWTGL
                     ARAVRSKVLSLREEEFAQAARLLGAKPRRIILRHLVPSFMSHLIASATLSIPGMILGE
                     TALSFLGLGVRAPMTSWGVLLNDAQNVNVVAIYPWLMLPVVPVIIVVLAFNFLGDGLR
                     DAADPYS"
     gene            1521..2495
                     /locus_tag="P5558_12130"
     CDS             1521..2495
                     /locus_tag="P5558_12130"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25103.1"
                     /translation="MTIYLRSALLGATALLAFSTGAKADCGEVTITEMNWASASVVTA
                     VSKFLMEEGYGCAVTVVPSDTVPAVTSVAETGKPDIVTELWINSTPAYVEMEKAGKVE
                     TLAAVLADGGIEGWFVPSYLIDEHPELATIDGVLANPDLVGGRFHNCPEGWGCRTKND
                     NFKVAYGLEEAGFEVFDHGSGETLATSLASAYEDRAPWFGYYWQPTSLLGRYEMVKVD
                     MGPYDAERHACIAREDCADPQRTDFQTADVVTGVTTDFAEREPAIAELMSKVSFTNAQ
                     MSELLAWQEQNKASAEEVAVKFLSENPDVWSAWLNDDARVNLAPLLSQ"
     gene            2588..3637
                     /locus_tag="P5558_12135"
     CDS             2588..3637
                     /locus_tag="P5558_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY25104.1"
                     /translation="MGYYDGLFDALGLRDWCGGGAAQDGPMTMAQLLARSRGGGEGEA
                     VATTPWWQVPFPSLDTLNASCPAIWRTRDLTAWVADGFLAIKNTLKVVLDPLTQPLSW
                     LLDGAIWAMLAVPWWVLVAILLVAVGLLTRSATLTASVCLSLLFLAFIDHFDYAMQTL
                     SIIFVCTTICVVLGVPIGIAMSKRDGFQRSMVPLLDMLQTLPTFVYLIPLIFLFSVTE
                     SKLYGIAIILYAIVPVIRLTDLGIRLVDKDVLEAADAFGMTPRQRLLGVEIPLALPNI
                     MAGINQTIMMALAMVVIASLVSAPGLGVLVLRGIRNLELGVGLIAGFSIVILAVILDR
                     VSKAAVGRISALTPG"
     gene            3658..4749
                     /locus_tag="P5558_12140"
     CDS             3658..4749
                     /locus_tag="P5558_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044224.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HRY25105.1"
                     /translation="MDMTEGNDPAEESDPKVSIRNLYKIFGDDPASVLAYAQAGMSKA
                     ELLAEHSHVLGLRDINVDMQAGEITVIMGLSGSGKSTLIRHLNRLIEPTAGQILVDGD
                     DVMTYGPLELRELRQTRMSMVFQKFALLPHRTVAENAGMALAIRGASRAEAAEEGGRW
                     LARVGLEGYGDYLPAQLSGGMQQRVGLARALASNAEIMLMDEAFSALDPLIRTEMQNL
                     LVELQNELHKTIVFITHDLDEALKLADHLVILKDGEIVQQGEPQGIVMQPADGYVTDF
                     VGDINRARVLRVRSVMTPTDAPADGAAGEIDCDATLEQMIALTGGDTSQNYIVTRDGD
                     PVGELDMKDLVKALAPETGTTGTNTAASP"
     gene            4833..6257
                     /locus_tag="P5558_12145"
     CDS             4833..6257
                     /locus_tag="P5558_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25106.1"
                     /translation="MWRWSSIFLGLAVLLLPCTGVRAQGPSADCGSPIGKWDWPTGGV
                     VTIVPGGQLSWAPVAGGPAVFTATWACDTNDGQIVIAWPQGITDRLRLSGDGERLDGA
                     NDRGVTLAARRWREVEAVVPAGPVPPALVGTWLLEVMLPTAEGPVAVVWTIAADGTYV
                     IDAGPSSHAGTMTAQGGNWEQAATTSDFTDGGTYALPNWARLQTRARSGMGGWNRIQP
                     DLVLPVIEVSGQILPSGLPELASRARMVARTWRPDAELVGIEFEQRDSNQWNTKDEAE
                     LRYVSPASGGGLVMTIATDGTWFFAHDVVNWGSGAIPDGFLDLPAVWAIARQHGLVPP
                     LARADLQVWQPGDAPILAWRLSSDRGEKRGVSLDGVGGTLLEGDLSGYVAAYNAQWQA
                     AAEGLRRLFAPARQASGWSSGSSGWDSGSSSSSSSSSSDSGSSSSSGYDTASQNSWGA
                     GDMDAYNRIQSGTPTGDDCYRYGC"
     gene            complement(6329..7642)
                     /locus_tag="P5558_12150"
     CDS             complement(6329..7642)
                     /locus_tag="P5558_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRY25107.1"
                     /translation="MTGTLASGPALAQDAAQAAVEAVAATGVSPESAFIFNTLLFLMT
                     GFLVMFMAAGFAMLEAGMVRKKSVATQCLKNVALFAIAGILYYLIGYSLMYDGVDGGW
                     MGTLAIWSPDDSAAAAGDFAAGYAASSDWFFQMVFVATAASIVSGTVAERIRLWPFLA
                     FVVILTGILYPITGSWQWGGGWLSELGFSDFAGSTLVHSVGGWAALAGALLLGARRGR
                     YGRDGSVTPMPASSLPLATLGTFILWLGWFGFNGGSQLAMGSGADAVKVATIFINTNM
                     AAAGGVIAAMIVCQLVYKKIDLTLVLNGAIGGLVSITAEPLAPSLGASMLIGGIGGVL
                     VVVAVPLLDKLKIDDVVGAIPAHLVCGIWGTLIVPFSNADTSYGTQLTGILAIGLFTF
                     IASFIVWGTLKATIGIRVHEEDEERGVDQAELGMEAYPEFGPSAV"
     gene            7935..9149
                     /locus_tag="P5558_12155"
     CDS             7935..9149
                     /locus_tag="P5558_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HRY25108.1"
                     /translation="MKGRFDLIIQGGGLTGLALAAAVGGVGGRVLLVEERPLSVTTAA
                     RFDGRVTAIARGSKQALGSIGAWAGMAEAAEAILEIEVGEADSLGSVHYDAAEVGGEP
                     LGWIVPNQAIRQGLLEAIAAAGSAVTIAAPASIETLARDAGGVEARLEDGRMVRGALL
                     AVCEGRFSGTRETLGIGARRWDYGQTGLVCTFAHELPHRGRAVERFFPDGPFAMLPLP
                     GNRSSIVWALESGLAKAVAALDDAAFTAEVQERFGDSLGRLALEGHRWSYPLILVWSH
                     AYVAERAVLVGDAARGIHPIAGQGWNLALRDVTAIAEIVVDRLRLGLDPGSADALERY
                     AAWRSFDSLALVAVTDGINRLFANDILPLKLARGAGLGLVERLPVAKRFFMRHAMGLV
                     GDLPRLMRGEAL"
     gene            9194..9919
                     /locus_tag="P5558_12160"
     CDS             9194..9919
                     /locus_tag="P5558_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY25109.1"
                     /translation="MPAASLVLLSAFTSALTTMAGIGGGVIMLGAMASLLPAAAIIPV
                     HAVVQLGSNIGRAAVLRRWIDTSRLAPFLLGSLVGIALGASLVVSLPVETLRLVLGLF
                     ILQTIWLPVAGMAAIKGRGLALGGAIASFLTMLVGATGPYVLALLRPLGLGREGLIAT
                     HAAAMAGQHGLKMLAFGLFGFAFLPWLPLVALMIAFGFAGTLMGRWLLGRIDPRHFAR
                     IIQVLLTVLALELVLRSLWALVR"
     gene            complement(9912..11327)
                     /gene="pyk"
                     /locus_tag="P5558_12165"
     CDS             complement(9912..11327)
                     /gene="pyk"
                     /locus_tag="P5558_12165"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRY25110.1"
                     /translation="MRRGRSTKIVATLGPASSTSEVIVALAKAGADVFRFNFSHGSHD
                     DHAERLDMVRAAEREIGRPIGVLLDLQGPKLRVGRLTQGRIGLTRGERFRLDLDDAVG
                     DQRRVQLPHPEIFAAIHPGTELLLDDGKIRLKVESCGPDHAETTVLAGGGLSDRKGVN
                     VPNVVLPIAAMTEKDRKDLQVGLDLGVDWVALSFVQRPEDMAEARRLVAGRAALMAKI
                     EKPAAIDRLDEIIELSDSIMVARGDLGVEMPPEVVPRLQKRIIRAAREAGKPVIVATQ
                     MLESMIQNPAPTRAEASDVATAVYDGADAVMLSAESAAGEYPVEAVAIMDRIIKEVEQ
                     DDLYRSYIEAFHAEAERTSADAITLAANQVAHTLQSAAIVTYTTSGATALRASRERPD
                     RPILGLITRMVTARKLALAWGVHAVFCEDAHDLDEMVQKARQHARAEGFAGPGASIVV
                     TAGLPFGTPGATNLLRIAWVT"
     gene            11488..12981
                     /locus_tag="P5558_12170"
     CDS             11488..12981
                     /locus_tag="P5558_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY25111.1"
                     /translation="MARSRHAPLYLASIALDPQGRAPLHRQLYFALRAAILEGRLPPG
                     ARLPSTRSLAADLAVSRNTVLAAFDQLHAEGYVQGRVGAGSFVSRQLPEDALQARRPG
                     ADAAASRAAAPPGPSRRGARLTALRQHGTRPRAFAPGLPELASFPFEDWSRLLARSWR
                     NPPRSLLIGSEPAGYRPLREALAAHLGAARAVRCTAEQVFIVSGAQQALDLAARVLLD
                     PGDRVWVEEPGYAGLIGALLAGGATVVPVPVDEQGLDVAGGRRRAATARMACVSPSHQ
                     FPLGLTMSLARRLELLDWARGADAFILEDDYDSEYRYAGRPLAALQGLDEDGRVIYVG
                     TMSKVMFPALRLGYMVVPLHLVDAFAAIRRLTDTHPSMLVQPALAEFIEDGSLAAHIR
                     RMRALYADRQALLLAAARRHLGERLALRPAEAGMHLVGYLEDGTDDRALAERAGRLGV
                     EVTPLAALYQERPDRSGLLLGYAGLHGSEIEHGLRGLARAFELPPAG"
     gene            13022..14902
                     /locus_tag="P5558_12175"
     CDS             13022..14902
                     /locus_tag="P5558_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY25112.1"
                     /translation="MEIGSRVLGDEIDELEPVPGRLRMMLRATVHCRHRLAPGLNPPE
                     KLGNRRAMPHVGAVMTISSEAERLSMGRSWYPTILAFAVALLLAGGGNAGPDRLTIGM
                     TQYPSTLNPNIDSMAAKSYVLGFTHRPFTQFDADWSLRCYLCTELPSLEAGTAEIVER
                     DDGTQGVRTTYTIDPQARWGDGTPVTTDDVAFTLEVGKHPDTGVSNAEMYRRIVDLEI
                     VDDKTFIFEDEKLGFQYDAINDFRLLPAHLERPVFEADPKTYRNRTLFDTDPTNPGLA
                     LGPYRIASLDSGGFIALERNPEWWGEPPAFDEIVIRTIENTAALEANLLSGEIDMVEG
                     SLGLSLDQAIAFDARHGDRFQVFYKPGLIYEHIDFNLDNPILADLRVRQALIQGIDRE
                     ALSQQLFGGRQAVAQTFVNPLDWVYTPDVLTYHEDLDEAGALLDAAGWTEWRDGIRHN
                     AAGEALRLTLMTTAGNRSRELVQQVLQSMWQRIGVDVRIQNEPARVFFGETMRQRRFP
                     ALAMYAWISSPENVPRTTLHSQEIPSADNSWTGQNTPGFRNARMDELIDEIEITLDRD
                     ERAPLWHELQRIYATELPVLPLYYRADPHIWPLWLEGVEPTGHLAPASLGVESWRVRD
                     DG"
     gene            14951..15907
                     /locus_tag="P5558_12180"
     CDS             14951..15907
                     /locus_tag="P5558_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25113.1"
                     /translation="MAGYFVRRLVETLLLLLVMSFLIYGLIGLMPGDPIDLMVSADPN
                     LTAADALRLKALQGLDVPLPERYWRWLSATLSGDLGYSRLYARPVLEVLGPRLLNTLL
                     LMGTSLILALVIAVPAGIVAALDPRGPVDNTINLLAFAGFSMPSFWLGLLLIILFSVQ
                     LGWLPAGGSASIGESGLLDRLRYLILPVLTLTLLTAGTFIRFVRAAMLEALREDFIRT
                     ARVKGLSTTAVVIRHALPHTALAMITITALHVGSLFSGALVVETIFAYLGMGKLIYDA
                     ILGNDYNLALVALLLATLVTLVANLVADLAYGWLDPRIDYGG"
     gene            15969..>16645
                     /locus_tag="P5558_12185"
     CDS             15969..>16645
                     /locus_tag="P5558_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25114.1"
                     /translation="MTAGWLLAALVLAGLVGPPLCRAFGLDGEAVDLFGRLAPASLEH
                     PLGTDELGRDLLLRLLEGGRISLAVGLAGALLTAVIGTAIGLAAGYAGGRVDAALMRL
                     TDAIIALPLLPLLIVLAAVDPIKLGLPAALADGGLASIWRIVLLVALFGWTTVARLVR
                     AAVLSQKERDYVRAARALGAGPWRIAVRHVLPNVASPIIVATTLSIGNIMLTESVLSF
                     LGLGIQPP"
BASE COUNT         2339 a         5777 c         5768 g         2761 t
ORIGIN      
        1 tgctcgcctg gctcgacccg cgcatccgct tcagcggcgg gcaggcccgc tgatgaccgt
       61 cgagccccgc cagccgccgg aatccgccgg cctccgaccc gccggcaaca gcgagcactg
      121 ggtcgacccc gagccctggc aggtgcatcg cgagcagccg ctgaccccgg agcaggagcg
      181 ctactacacc gcctcgcaat ggcggctgat gtggtggcgc ttcaagcggc acaaggtggc
      241 ggtggcgtcc ttgtgggtgc tcgttctctt ctatgtggtc gtggtgatcg ccgaggtggt
      301 cgcaccctac gacgcgcaga cccgggaccc gctgcacatc catgcaccgc cccagccgat
      361 ccatctcttt catgaagggc ggttcgtcgg cccgttcacc tacgggtatc agatgacgct
      421 cgacatgacg acgctgaagc ggatctacga ggcggaccgg acgatggtcc agccgctgcg
      481 cttcttctgc cggggcgatg cctaccgctt ctggggccag tggcccggcg acttccacct
      541 cgtctgtccg gccgagggcg gcaccttctt cctcctcggc accgaccggc tcggccgtga
      601 catgttctcg cggattctct acggggcccg ggtctcgctg acggtcggcc tcgtcggcat
      661 cgccatcagc ttcgcgctcg gcatcatcct cggcggtgtt tccggctatt acggcggctg
      721 gatcgacaat atcgtgcagc ggctcatcga ggtgatccgc agctttccga tgatcccgct
      781 ctggctcgcc ctctcggcgg ccctgccacc gacctggccg cccatctggg tctatttcgg
      841 catcacgctc attctcggcc tcgtcgactg gacgggcctg gcgcgggccg tccgctccaa
      901 ggtgctgtcg ctgcgcgagg aggagttcgc ccaggccgcc cgcctcctgg gtgccaagcc
      961 gcggcggatc atcctccgcc acctggtgcc gtccttcatg agccacctga tcgcctcggc
     1021 gacgctttcc atcccgggca tgatcctcgg cgagacggcg ctctccttcc tcggcctcgg
     1081 tgtcagggcg ccgatgacga gctggggcgt gctgctcaac gacgcccaga acgtcaatgt
     1141 ggtcgccatc tatccctggc tgatgctgcc ggtcgtcccg gtcatcatcg tcgtcctcgc
     1201 cttcaacttc ctcggcgacg gattgcgcga cgcggccgac ccctacagtt gagccggcaa
     1261 ccggcctcgg gttgacagcc gggctgcttc gcgccaagct gcgttatctt ctgtcgctgg
     1321 aatttgcgca gttgccgtga tttatcgcgg cgttgtcgtg cgtgcgcggt gcattcgggg
     1381 ttcagtatca cccgttctag atgtcgagtc gtcctggttc atattgggcc tgagcgtcga
     1441 ccttgcacgt ctgcggtcgg cgctgttctt gcccgtggca gaagatctgc tttcggcccg
     1501 gaatcacaag gaactcatcc atgacgatct atctcaggtc tgcgctgctc ggcgcaacgg
     1561 ccctgcttgc cttttcgacc ggtgccaagg ccgattgcgg cgaggtcacg atcaccgaga
     1621 tgaactgggc atcggcttcg gtggtcacgg cggtttccaa gttcctgatg gaggaaggct
     1681 acggctgcgc cgtgacggtc gtgccatcgg acaccgtgcc ggccgtgacc tcggtcgccg
     1741 agaccggcaa gcccgacatc gtcaccgagc tctggatcaa ctcgactccg gcatatgtcg
     1801 agatggagaa ggcgggcaag gtcgagaccc tggcggccgt cctggcggat ggtgggatcg
     1861 agggttggtt cgtgccgtcc tatctgatcg acgagcatcc cgagctggcc accatcgacg
     1921 gtgtcctggc caaccccgac ctggtcggcg gccggttcca caactgcccc gagggctggg
     1981 gctgtcgcac caagaacgac aatttcaagg tcgcctatgg tctcgaggag gccggcttcg
     2041 aggtcttcga ccacggatcg ggcgagacgc tcgccacctc gctggcctcg gcctacgagg
     2101 acagggctcc ctggttcggc tattactggc agccgacctc gctactcggc cgctacgaga
     2161 tggtgaaggt cgacatgggc ccctacgacg ccgagcggca tgcctgcatc gcccgtgagg
     2221 attgtgccga cccgcagcgg accgatttcc agaccgctga cgtcgtgacc ggtgtcacga
     2281 cggatttcgc cgagcgtgag ccggcgatcg ccgagctcat gagcaaggtg agcttcacca
     2341 acgcgcagat gagcgagctg ctggcctggc aggagcagaa caaggcttcg gccgaggagg
     2401 tggcggtcaa gttcctgagc gagaacccgg acgtctggtc ggcctggctg aacgatgacg
     2461 ccagggtcaa cctcgccccg ctgctcagcc agtaggtgcg ccggatgggg tggcgctccg
     2521 ctaacggagc gccaccccgt tcccgagctc gattaagcgg accgaaacaa ctcgaggtcg
     2581 cgtgcggatg gggtattacg acgggctgtt cgatgcgctc gggctgcgcg actggtgcgg
     2641 tggtggtgcg gcgcaggacg ggccgatgac catggcccag cttctggcca ggagtcgcgg
     2701 cggtggcgag ggtgaggcgg tggcgaccac cccctggtgg caggtgcctt tcccgtcgct
     2761 cgacacgctc aatgcctcgt gccccgcgat ctggcgcacg cgcgatctca ccgcctgggt
     2821 cgccgatggc ttcctcgcga tcaagaacac gctcaaggtc gtcctcgatc ccctgaccca
     2881 gcccctctcc tggctgctcg acggtgccat ctgggcgatg ctggcggtgc cctggtgggt
     2941 gctggtggcg atcctcctgg tggcggtcgg cctgttgacg cgctcggcga cgctgacggc
     3001 gtccgtgtgc ctgtcgctgc tgttcctcgc cttcatcgac catttcgact acgcgatgca
     3061 gacactctcc atcatcttcg tctgcacgac gatctgcgtc gtgctagggg tgccgatcgg
     3121 catcgccatg tccaagcgcg acggctttca gcgcagcatg gtgccgcttc tggacatgct
     3181 ccagaccctg ccgaccttcg tctacctgat cccgctgatc tttctcttca gtgtcaccga
     3241 gtcgaagctc tacggcatcg ccatcattct ctacgccatc gtgccggtga tccggctgac
     3301 cgatctcggc atcaggctgg tggacaagga cgtgctcgag gcggccgatg ccttcggcat
     3361 gacaccgcgc cagcgcctcc tcggcgtcga gatacccctg gccctgccca acatcatggc
     3421 cggtatcaac cagacgatca tgatggcgct ggcgatggtg gtgatcgcgt ccttggtctc
     3481 ggccccgggg ctgggtgtcc tggtgctgcg cggcatccgc aatctcgagc tcggcgtcgg
     3541 gctgatcgcc gggttcagca tcgtcatcct ggcggtgatc ctggaccggg tgagcaaggc
     3601 ggcggtcggg cggatcagcg cgttgacgcc cggttgagcg cgatgcagga gcattcgatg
     3661 gatatgaccg aagggaacga ccccgccgag gagagcgacc ccaaggtctc gatccgcaat
     3721 ctctacaaga tcttcggcga cgatccggcg tccgtgctcg cctatgcgca ggccgggatg
     3781 agcaaggcgg agctgctcgc cgagcacagc catgtcctcg ggctgcgcga tatcaatgtc
     3841 gacatgcagg ccggcgagat caccgtcatc atggggctct cgggctccgg caagtcgacg
     3901 ctgatccgcc atctcaatcg cctgatcgag ccgaccgcag ggcagatcct ggtggatggc
     3961 gacgacgtga tgacctacgg ccccctggag ctgcgcgagc tgcgccagac gcgcatgtcg
     4021 atggtgttcc agaaattcgc tctcctgccg caccgcacgg tcgccgagaa cgccggcatg
     4081 gcgctcgcca tccgtggcgc cagtcgggcc gaggcggcgg aggagggggg gcggtggctc
     4141 gcccgggtgg ggctcgaggg ctatggcgac tatctgccgg cgcagctctc gggcggcatg
     4201 cagcagcgcg tgggcctggc tcgcgccctg gcctcgaatg ccgagatcat gctgatggac
     4261 gaggcgttca gcgccctcga cccgctgatc cgcaccgaga tgcagaacct gctggtcgag
     4321 ctgcagaacg agctgcacaa gaccatcgtc ttcatcaccc acgacctcga cgaggcgctc
     4381 aagctcgccg accatctggt gatcctgaag gacggcgaga tcgtccagca gggcgagccg
     4441 cagggcatcg tcatgcagcc ggccgacggt tacgtcaccg atttcgtcgg cgacatcaac
     4501 cgggcccggg tgctgcgcgt ccgctcggtg atgacgccga ccgatgcgcc ggccgatggt
     4561 gccgccggcg agatcgactg cgacgcgacg ctcgagcaga tgatcgcgct caccggcggc
     4621 gacacctcgc agaattatat cgtcacccgc gacggcgacc cggtcggcga gctcgacatg
     4681 aaggatctgg tcaaggcgct cgccccggag acgggcacga ccgggacgaa cacagccgca
     4741 tccccctgat acgcgacctc gtggtcggcc ggtttggccg ctccgggcgg tcgtgataag
     4801 ctctttagtc gaggtatgtc ggggaggcgg ccatgtggcg ctggtccagc attttccttg
     4861 gtcttgcggt gctgctgctg ccctgcacag gtgtccgggc gcagggtccg agcgccgatt
     4921 gcggctcgcc gatcggcaag tgggactggc cgaccggcgg tgtcgtcacc attgtcccgg
     4981 gcgggcagct ctcctgggcg ccggtcgccg gcggtcccgc tgtcttcacg gcaacctggg
     5041 cgtgcgacac gaatgacggc cagatcgtca tcgcctggcc gcagggcatc accgaccggc
     5101 tgcgtctttc gggcgatggc gagcggctgg atggcgccaa cgaccggggc gtgacgctgg
     5161 cggcgcggcg ctggcgcgag gtcgaggcgg tggtgccggc cgggccggtg ccgccggcgc
     5221 tggtcggcac ctggctgctc gaggtcatgc tgccgacggc ggaggggccc gtggcggtgg
     5281 tctggacgat cgccgcggac ggcacctatg tgatcgatgc cgggccctcg agccatgcgg
     5341 ggacgatgac ggcccagggc ggcaactggg agcaggcggc gacgaccagc gacttcaccg
     5401 acggcggcac ctacgccctg cccaactggg cgcggctgca gacccgggcg cggagcggca
     5461 tgggcggctg gaaccggatc cagccggatc tggtcctccc ggtgatcgag gtgagcggcc
     5521 agatcctgcc ttccggcctg cccgagctgg cgagccgggc acgaatggtg gcgcggacct
     5581 ggcgacccga tgccgagctg gtcgggatcg agttcgagca gcgggacagc aaccagtgga
     5641 acacgaagga cgaggccgag ctgcgctacg tctcacccgc ctcgggcggc gggctggtga
     5701 tgaccatcgc caccgacggc acttggttct tcgcccacga cgtcgtcaac tggggtagcg
     5761 gcgccattcc cgacgggttc ctcgaccttc ccgcggtctg ggcgatcgcc cgccagcacg
     5821 gcctggtgcc gccgctggcg cgcgccgacc tccaggtctg gcaaccgggc gacgcgccga
     5881 tcctcgcctg gcggctctcc tccgaccgcg gcgagaagcg gggcgtttcg ctggatggcg
     5941 tcggcggcac gctgctcgag ggcgatctct cgggctatgt cgccgcctac aacgcgcagt
     6001 ggcaggcggc ggcggagggc ttgcgccggc tcttcgcccc ggcccgccag gcatccggat
     6061 ggagcagcgg ctcgagcggc tgggacagcg gcagcagctc cagctcgagc agcagcagct
     6121 cggacagcgg cagcagcagc tcgtcgggct acgacacggc gtcgcagaac tcctggggtg
     6181 ccggcgacat ggacgcctac aaccgcatcc agagtggcac gccgaccggc gacgactgct
     6241 accgctacgg ctgctgagcg gtcgatgacg aaaaaatggc ccgggaggcg acctcccggg
     6301 ccatgaggtg cgctgtcgga cccgcaggtc agacagcgct cgggccaaac tccggatagg
     6361 cttccatgcc gagctccgcc tggtcgacgc cgcgctcctc gtcctcctcg tgaacccgga
     6421 tgccgatcgt cgccttgagg gtgccccaga cgatgaagct ggcgatgaag gtgaagaggc
     6481 cgatggcgag gatgccggtg agctgcgtgc cgtagctcgt gtcggcgttg ctgaagggga
     6541 cgatcagcgt gccccagatg ccgcagacga gatgcgccgg gatggcgccg acgacgtcgt
     6601 cgatcttcag cttgtcgagc agcggcacgg cgacgacgac cagcacgccg ccgatgccgc
     6661 cgatgagcat cgaggcaccc agcgacgggg cgaggggctc ggcggtgatc gagacgaggc
     6721 cgccgatggc gccattgagg accagcgtca ggtcgatctt cttgtagacg agctggcaga
     6781 cgatcatcgc cgcgatcacg ccgccggcgg ccgccatgtt ggtgttgatg aagatcgtgg
     6841 caaccttcac cgcgtcggcg ccgctgccca tggcgagctg ggagccgccg ttgaagccga
     6901 accagccgag ccagaggatg aaggtgccga gcgtggcgag cggcagcgag ctcgccggca
     6961 tcggcgtgac cgagccgtca cggccatacc ggccgcgcct cgcgccgagc aggagggcac
     7021 cggcgagagc ggcccagccg ccgaccgagt ggaccaacgt ggagccggcg aagtcggaga
     7081 agcccaactc gctgagccag ccgccgcccc attgccagga cccggtgatc ggatagagga
     7141 ttcccgtcag gatcacgacg aaggcgagga acggccagag cctgatccgc tcggcgacgg
     7201 tgcccgagac aatggacgcc gcggtcgcga cgaagaccat ctggaagaac cagtcggacg
     7261 aggcggcata gccagcggcg aaatcgccgg cggcggcggc gctgtcgtcg gggctccaga
     7321 tggcgagcgt gcccatccag ccgccatcga ccccgtcata catgaggctg tagccgatga
     7381 ggtagtagag gatgccggcg atcgcgaaga gggcgacgtt cttcaggcac tgggtggcga
     7441 cgctcttctt gcgcaccatg ccggcctcga gcatggcgaa gccggcggcc atgaacatga
     7501 cgagaaagcc ggtcatgagg aagaggaggg tgttgaagat gaaggcgctt tccgggctga
     7561 cgccggtggc cgcgacggcc tcgacggcag cctgggccgc atcctgcgcc agggccgggc
     7621 cgctggcgag cgtgcccgtc atggcgatcc cgaatccgag gcagactgcg gccgggacag
     7681 gtcgcccacg ccgtgatagg tatcgatgca tgtgtctctc ccgccatacc gatgcgattg
     7741 agtgtctggt gcggcgccgc gccggtgtgg tcatggcgac gacttctcgg atcgacagga
     7801 gcaagctccg tgccagcggt ggcggcggcc ttgtacggcc cgggatcgaa gcgaaggcag
     7861 gtcgagcgga tccggagagt gcctagaatt tcacccggtg cgtatttcct gagcaacagg
     7921 gtcggagcat gagcgtgaag gggcggttcg atctgatcat ccagggcggt gggctcaccg
     7981 gcctcgccct ggcggccgcc gtgggcggcg tgggcggccg ggtgctgctg gtcgaggagc
     8041 ggccgctgtc ggtgacgacg gccgcccggt tcgacggccg ggtgaccgca atcgcccgtg
     8101 gctcgaagca ggcgctcggt tcgatcggcg cctgggccgg catggccgag gcggccgagg
     8161 cgatcctcga gatcgaggtg ggcgaggcgg attcgctggg ctcggtgcac tacgacgcgg
     8221 ccgaggtggg cggcgagccc ctgggctgga tcgtgccgaa ccaggcgatc cgccagggcc
     8281 tgctcgaggc gatcgccgcc gccggctcgg ctgtgaccat cgccgcaccg gcgagcatcg
     8341 agacactcgc gcgcgatgcc ggcggcgtcg aggcacgcct cgaagatggc cgaatggttc
     8401 gaggagcgct gctcgccgtc tgcgagggtc gcttctcggg cacccgtgag acgctcggca
     8461 tcggtgcccg gcggtgggac tacggccaga ccgggctggt ctgcaccttc gcccacgagc
     8521 tgccgcacag gggccgggcc gtggagcgct tcttcccgga cggccccttc gccatgctgc
     8581 ccctgccggg caatcgctcg tccatcgtct gggcgctgga gagcgggctg gccaaagcgg
     8641 tggcagcgct cgacgacgcg gccttcacgg ccgaggtgca ggagcgcttc ggcgacagcc
     8701 tcgggcggct cgcgctcgag ggccatcgct ggtcctatcc cttgatcctc gtctggtcgc
     8761 acgcctatgt cgccgagcgc gcggtgctcg tcggcgatgc ggcccggggc atccatccga
     8821 tcgccgggca ggggtggaac ctggcgctcc gcgatgtgac ggcaatcgcc gagatcgtcg
     8881 tcgatcgtct ccggctcggg ctcgatccgg gcagcgccga cgcgctcgag cgctatgccg
     8941 cctggcgctc cttcgacagc ctggcgctgg tggcggtcac cgacggcatc aaccgcctct
     9001 tcgccaacga tatcctgccc ctgaagctcg cccgcggcgc cggcctcggc ctggtcgagc
     9061 gcctgccggt cgccaagcgg ttcttcatgc gccacgccat ggggctggtc ggcgacctgc
     9121 cccggctgat gcgcggcgag gcgctctgac cgtatacctc gtcgacgggt tcctgccgga
     9181 cgggctgggt ctcgtgccgg ccgcctcgct cgtcctgctc tccgccttca cctcggcgct
     9241 gaccaccatg gccggcatcg gcggcggcgt catcatgctc ggcgccatgg cgagcctgct
     9301 gccggcggcg gcgatcattc cggtccatgc cgtggtgcag ctcggctcga acatcggccg
     9361 ggcggcggtg ctgcggcgct ggatcgacac gagccggctc gcccctttcc tgctcggcag
     9421 cctcgtcggc atcgcgctcg gggccagtct cgtcgtcagc ctgccggtcg agacgctgcg
     9481 cctcgtgctc gggctcttca tcctgcagac gatctggctg ccggtcgccg gcatggcggc
     9541 gatcaagggg cggggcctgg cgctgggcgg tgcgatcgcc tccttcctca ccatgctggt
     9601 cggcgccacc gggccttacg tcctggccct gctgcgcccg ctcggcctcg gccgggaagg
     9661 gctgatcgcc acccacgcgg cggcgatggc cggccagcac gggctgaaga tgctcgcctt
     9721 cgggctcttc ggcttcgcct tcctgccctg gctgccgctg gtcgccctga tgatcgcctt
     9781 cggcttcgcc ggcacgctca tgggccgctg gctgctcggc cggatcgatc cccgccattt
     9841 cgcccgcatc atccaggtgc tgctgacggt gctggcgctg gagctggtgc tgcgctcgct
     9901 ctgggcgctc gtcaggtgac ccaggcgatt ctgaggaggt tggtggcgcc gggcgtgccg
     9961 aagggcagcc cggcggtcac cacgatgctg gcgccggggc cggcgaagcc ttcggccctg
    10021 gcgtgctggc gggccttctg caccatctcg tcgaggtcgt gggcgtcctc gcagaagacg
    10081 gcgtggacgc cccaggcgag cgccagcttc cgcgccgtca ccatgcgggt gatgaggccg
    10141 aggatgggcc ggtccggccg ttccctggaa gcgcgaaggg cggtggcgcc cgaggtggtg
    10201 taggtgacga tcgccgccga ttgcagggta tgggcgacct ggttggcggc gagcgtgatg
    10261 gcgtcggcgg aggtgcgctc ggcctcggcg tggaacgcct cgatatagga gcggtagagg
    10321 tcgtcctgct ccacctcctt gatgatccgg tccatgatgg cgaccgcctc gaccggatac
    10381 tcgccggccg cgctctcggc cgagagcatc accgcgtcgg cgccgtcata gacggcggtc
    10441 gccacgtccg aggcctcggc ccgggtgggg gccgggttct ggatcatgct ctccagcatc
    10501 tgggtggcga cgatcaccgg cttgccggcc tcgcgcgcgg cgcgaatgat tctcttctgc
    10561 aggcgcggca ccacctcggg cggcatctcg acgccgaggt cgccgcgcgc caccatgatg
    10621 gaatccgaga gctcgatgat ctcgtcgagc cggtcgatgg ccgccggctt ctcgatcttc
    10681 gccatgagcg ccgcccggcc ggcgaccagg cggcgcgcct cggccatgtc ctcgggccgc
    10741 tggacgaagc tcaaggccac ccagtcgacg ccgaggtcga gcccgacctg caggtccttg
    10801 cggtccttct cggtcatcgc cgcgatcggc agcaccacgt tgggcacgtt cacgcccttg
    10861 cggtcggaga gcccgccgcc ggcgagcacc gtggtctcgg catgatccgg gccgcagctc
    10921 tccaccttca ggcggatctt gccgtcgtcg agcaggagct ccgtgccggg atggatggcg
    10981 gcgaagatct cgggatgcgg cagttgcacc cggcgctggt cgcccacggc gtcgtcgagg
    11041 tcgaggcgaa agcgctcgcc ccgggtcagc ccgatccggc cctgggtcag tcggccgacg
    11101 cgcagcttcg gcccctgcag gtcgaggagg acgccgatgg gccggccgat ctcacgctcc
    11161 gccgcgcgca ccatgtccag gcgctcggca tgatcgtcgt ggctgccatg gctgaagttg
    11221 aagcgaaaga cgtcggctcc cgccttggcc aatgccacga tcacttcgct ggtcgagctg
    11281 gccggaccga gggtggcgac gatcttggtg ctgcgcccac gtcgcatgcc gtcctccctg
    11341 atgtgcttcc gccgtcaata gacgaagcgg tgccgggtgt cacggtcgcg cgctcgggtg
    11401 ccaaatggct tgtccatggc ggcgagatgg acaagaacgg cagggccatt tcgtgcgccc
    11461 gcgcccgtcc attgaaagac ccggcagatg gcccgatccc gccacgctcc gctctatctg
    11521 gcctccatag cgctcgaccc gcaagggcgg gcaccgctgc atcgccagct ctacttcgcg
    11581 ctccgggccg ccatcctcga aggccgtctg ccgcccggcg cccggctgcc ctcgacccgc
    11641 tcgctcgccg ccgacctcgc cgtgtcgcgc aacaccgtgc tcgcggcctt cgaccagctg
    11701 cacgccgagg gctatgtcca gggaagggtg ggggcgggct cgttcgtcag ccgccagctt
    11761 ccggaggatg cgctccaggc caggcggccg ggcgccgacg ccgcggcgag ccgggccgca
    11821 gcaccgcccg ggccgtcgcg ccgcggtgcc cggctcaccg cgctgaggca gcacggcacc
    11881 cgtccgcgcg ccttcgcgcc ggggctgccg gagctcgcga gctttccctt cgaggactgg
    11941 tcgcggctgc tcgcccggag ctggcgcaac ccgccgagga gcctcctgat cggcagcgag
    12001 cccgccggct accggcccct gcgcgaggcg ctggcggcgc atctgggggc ggcgcgggcg
    12061 gtccgctgca cggccgagca ggtcttcatc gtctccggcg cccagcaggc gctcgacctg
    12121 gcggcccggg tgctgctcga tccgggtgac cgggtctggg tcgaggagcc gggctatgcc
    12181 ggcctgatcg gggccctcct ggcgggcggg gcgacggtcg tgccggtgcc ggtggacgag
    12241 cagggcctcg acgtcgccgg cggtcgccgg cgcgccgcca cggcgcggat ggcctgcgtc
    12301 tcgccctcgc accagtttcc gctcgggctc accatgagcc tggcgcggcg gctcgagctc
    12361 ctggactggg cgaggggggc ggatgccttc atcctcgagg acgactacga cagcgagtac
    12421 cgctatgccg gccggccgct cgccgccctg cagggcctcg atgaggacgg ccgggtcatc
    12481 tatgtcggca ccatgagcaa ggtcatgttc ccggccctgc gcctcggcta catggtggtg
    12541 ccgctgcatc tcgtggatgc cttcgcggcg atccggcggc tgaccgacac ccacccctcg
    12601 atgctggtcc agcctgccct cgcggagttc atcgaggacg gctcgctcgc cgcccacatc
    12661 cgccggatgc gcgccctcta tgccgaccgg caggcgctgc tgctggctgc ggcacggcgt
    12721 catctgggcg agcggctggc gctgcggccg gccgaggccg gcatgcatct ggtgggctat
    12781 ctcgaggacg gcacggacga ccgcgccttg gcggaacggg ccgggcggct cggcgtggag
    12841 gtgacgccgc tcgccgccct ctaccaggag cggcccgacc gcagcggtct cctgctgggc
    12901 tatgccgggc tccatggcag cgagatcgag cacgggttgc ggggcctcgc ccgcgccttc
    12961 gagctgccgc ccgccggctg agaaggcgag gcgcctgcgg ccgccgcgcc agcaggacgc
    13021 tgtggaaatc ggcagccggg tcctcggcga cgaaatcgac gagctggagc ccgttccggg
    13081 tcgactgcgc atgatgctgc gggccaccgt tcactgccgt caccggctcg ctcccgggtt
    13141 gaacccgccc gaaaagcttg gcaaccgccg cgccatgccg catgtcggtg cggtcatgac
    13201 catctccagc gaggcggagc gactgtccat gggccgatcc tggtatccga cgatcctggc
    13261 cttcgccgtt gccctgctgc tcgccggcgg cggaaatgcc gggcccgacc gcctgaccat
    13321 cggcatgacg cagtatccct cgaccctcaa ccccaatatc gacagcatgg cggccaagag
    13381 ctacgtcctg gggttcacgc atcggccgtt cacccagttc gacgccgact ggagcctgcg
    13441 ctgctatctc tgcaccgagc tgcccagcct cgaggccggg acggcggaga tcgtcgagcg
    13501 cgacgacggt acgcaggggg tgcgcaccac ctacaccatc gatccgcaag cgcgctgggg
    13561 cgacggcaca ccggtgacga cggacgacgt cgccttcacc ctcgaggtcg gcaagcatcc
    13621 ggataccggc gtcagcaatg ccgagatgta ccggcgcatc gtcgatctcg agatcgtcga
    13681 cgacaagacg ttcattttcg aggacgagaa gctcggcttc cagtacgacg cgatcaacga
    13741 tttccggctc ctgccggcgc acctcgagcg gccggtcttc gaggccgatc ccaagaccta
    13801 ccgcaaccgg acgctcttcg acaccgatcc gacgaatccc ggcctggcgc tcggtcccta
    13861 ccgcatcgcc agcctcgatt cgggcggctt catcgcgctg gagcgcaacc ccgaatggtg
    13921 gggcgagccg cctgccttcg acgagatcgt catccgcacc atcgagaaca cggccgccct
    13981 cgaggcgaac ctgctctccg gcgagatcga catggtggag ggcagcctcg gcctctctct
    14041 cgaccaggcg atcgccttcg atgcccgcca cggcgaccgt ttccaggtgt tctacaagcc
    14101 cgggttgatc tacgagcata tcgacttcaa cctcgataac ccgatcctcg ccgacctgcg
    14161 ggtgcgccag gcgctcatcc agggcatcga ccgcgaggcc ctgagccagc agctcttcgg
    14221 cggtcggcag gcagtggcgc agactttcgt caacccgctc gactgggtct acacccctga
    14281 cgttttgacc tatcacgagg acctggacga ggcaggggca ctgctggatg ccgccggctg
    14341 gaccgagtgg cgtgacggaa tccgccacaa cgcggccggc gaggccctgc ggctgacgct
    14401 gatgaccacc gccggcaacc gctcgcgcga gctcgtccag caggtgctgc agtcgatgtg
    14461 gcagcgtatc ggggtcgacg tgcgcatcca gaacgagccg gcccgggtct tcttcggcga
    14521 gacgatgcgc cagcggcggt tcccggcgct cgccatgtat gcctggatct ccagccccga
    14581 gaacgtgccg cggaccacgc tgcattcgca ggagattccc tccgccgaca acagctggac
    14641 cgggcagaac acgccgggct tcaggaacgc gcggatggac gagctgatcg acgagatcga
    14701 gatcacgctc gaccgggacg agcgggcccc gctctggcac gagctgcagc gcatctacgc
    14761 gaccgagctg ccggtcctgc cgctctacta ccgcgccgac ccgcatatct ggcccttgtg
    14821 gctcgagggc gtcgagccga ccgggcatct ggcgccggcc agcctcgggg tcgagagctg
    14881 gcgggtccgc gacgatggct gaggcggcgc cgcgggcccc gctcgaccgc cactgctgaa
    14941 ggggcgctga atggcgggct atttcgtccg gcgcctggtc gagaccctcc tcctcctgct
    15001 cgtcatgtcg tttctgatct atggcctgat cgggctgatg ccgggcgatc cgatcgatct
    15061 catggtcagc gccgacccga acctgaccgc tgctgacgcc ctcaggctca aggcgctgca
    15121 ggggctggat gtgccgctgc ccgagcgcta ctggcgctgg ctttcggcca cgctctcggg
    15181 cgatctcggc tattcccgcc tctatgcaag gccggtgctc gaggtgctcg ggccgcgcct
    15241 gctcaacacc ctgctcctca tgggcaccag cctgatcctc gccctcgtga tcgccgtgcc
    15301 ggccggcatc gtggccgccc tcgacccgcg cgggccggtc gacaacacga tcaatctcct
    15361 ggccttcgcc ggcttctcca tgccgtcctt ctggctgggc cttctgctca tcatcctttt
    15421 ctcggtgcag ctcggctggc tgccggccgg cggcagcgcc tcgatcggcg agagcgggct
    15481 gctcgatcgg ctgcgctacc tgatcctgcc ggtgctgacg ctgacgctgc tcaccgccgg
    15541 cacgttcatc cgcttcgtcc gggcggccat gctggaggcg ctgcgcgagg acttcatccg
    15601 cacggcccgg gtcaagggcc tctcgaccac ggcggttgtc atccgccacg ccttgccgca
    15661 cacggcgctg gcgatgatca ccatcaccgc cctgcatgtc ggctcgctct tctcgggtgc
    15721 cctcgtggtc gagacgatct tcgcctatct cggcatgggc aagctcatct acgacgccat
    15781 cctcggcaac gactacaacc tggcgctggt ggcgctgctg ctcgccacgc tcgtcacgct
    15841 cgtagccaat ctcgtcgcgg atctcgccta tggctggctc gacccgcgga tcgactatgg
    15901 cggctgagcg ccttgcggtc gggcgaagac ggcgcctctg gcggcgcttt gccggccatc
    15961 gcctggccat gacggcgggc tggctgctcg ccgcgctggt gctcgccggg ctcgtcggcc
    16021 cgccgctttg ccgggccttc gggctggacg gcgaggcggt cgacctcttc ggcaggctgg
    16081 ccccggccag cctcgagcac ccgctcggca ccgacgagct cggccgcgac ctgctgcttc
    16141 gcctgctcga gggcggccgg atctcgctcg ccgtgggcct cgccggtgcc ctgctcaccg
    16201 cggtgatcgg cacggccatc ggcctcgccg ccggctatgc gggcgggcgg gtggatgcgg
    16261 ctctcatgcg gctgaccgat gcgatcatcg ccctgccgct gctgccgctc ctgatcgtgc
    16321 tcgccgccgt cgacccgatc aagctcggcc tgccggcggc gctcgccgat ggcgggctcg
    16381 ccagcatctg gcgcatcgtg ctgctcgtgg cgctcttcgg ctggaccacc gtggcccggc
    16441 tggtgcgcgc cgccgtgctg agccagaagg agcgcgacta cgtgcgcgcc gcgcgcgccc
    16501 tcggtgccgg gccctggcgg atcgccgtcc gccacgtcct gccgaacgtc gcctcgccga
    16561 tcatcgtcgc cacgacgctc tcgatcggca acatcatgct gaccgaatcc gtcctgagct
    16621 ttctcggcct cggcatccag ccgcc
//
LOCUS       DAOTFI010000106        16390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4274_length_16390_cov_7.306765, whole genome shotgun sequence.
ACCESSION   DAOTFI010000106 DAOTFI010000000
VERSION     DAOTFI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 16390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16390
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4274_length_16390_cov_7.306765"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3410)
                     /locus_tag="P5558_12190"
     CDS             complement(<1..3410)
                     /locus_tag="P5558_12190"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRY25115.1"
                     /translation="MIRWQRKGLAALALGMALGGLSPAERASAQTVPLNVVYGPVAQT
                     REGDLDHRENLFISVPADLEGHLFLRLFDPEANGLHDTAYGSFGNSTVLFRLLGGAGA
                     YTGVEFPPPVADGSPADPEAGLPLPDEAGVLIAEQRFGAEPATDDRWVTLTPFQASQG
                     EIRDGRAYFRLDVIGEAGDDGNVFAFEVSRAPDASELPADVRLFAFRPTLRWPGTREV
                     VELRFSAPAGERATLQNFDGAEASVELVSTFASTALPASGQDAWASGTFTVPEGETAI
                     EFKGGNETPNDITFALFDEAGGPLPFELPARLRQPALRPEAVGIATPLADCRAVAFDA
                     SGSSGDHPLRLQWLFGDGRNSAETVVVHRYDIPGRYEAELQVLGPSDRVASGSSIRLP
                     VHVRNRPTAVAGEAVVAAPGDAILFDGSASLPSDRPISSFLWSFGDGSQASGAEARKT
                     YATPGLYRSVLRVADDADHPCNFGIAQRLVTINFQPTAEAGEARSAAVGEAIRFDGGA
                     SYDVDGEIAGWHWAFGDGDSASTAAADHAFQAPGVYEARLTVVDDAGVGNSSTSDTVR
                     ITVNAPPVPVSTQPTRPIAVGEIARFDATQSTDPDGEILDYQWAFGDGAIAEGAVVEY
                     AYGAPGIYEVRLTARDNSATRSDTAETSFQVVVSAAPVADAGPDQLVTASEVVFDGGG
                     SSDADGTITSYEWTFGDGATGTGRVMRHAYVEPGTYEVALTVRDDSGAPLNVHRDTAM
                     VRVNQAPIADAGPDLVAAPGQSLTFDGRGSLDPDGVIDIYAWRFGDGSDGQGAQIRHS
                     FDAPGHYRVELTVRDDSGQEAAFDVDEARVTINAAPVADAGPDVLIAPGTELRLSSAA
                     SFDPDGSIEVHRWDFDDRATPVLERSPRRVFDEPGIYTAQLTVSDGSGTLNASSSDEM
                     LIHVNHAPVAEAGAEIRTDQLIVTLDGSGSVDADGDTLVFTWDPGDGSPALTGRTVTH
                     AYPRGGVFPVTLTVDDGTGLANSTAVDATRVVIDSRPVAVAGGNHDVCSGESILFDGS
                     ASSDPDGGLLRYEWDFGDGTRANLVNPAKTYELPGVYPVTLTVRDESGNASGVHSDRV
                     AAVVREAPIAVAGAPTRACTNQTIRFDGSHSTDADGAVNLFSWNFGDGS"
     gene            complement(3475..5790)
                     /locus_tag="P5558_12195"
     CDS             complement(3475..5790)
                     /locus_tag="P5558_12195"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY25116.1"
                     /translation="MRWIGAALLLGAPAALAVVEIDGPLTTVRVEQGQSIRDLAALYL
                     KDPDLWPQIIEFSGLGSVVDVRPGIELKVPVVQVAAADDALAISLVSIQKATAEGARI
                     FAPIEIGTAIEKRDEAVEYRTAGEWREVVVHANEATAHAEQALDISLQQRDRAAEAVL
                     SDIHGAVEGSKPVETGWSGRRLNDFLIEFERLRTLSASTAQVTFRDLSRLRLNPNSNA
                     VIQRMRSDPLTGAETTKVSLVEGDFYALLNQLHNRADFEVEVPGVETEATSADFWVSH
                     DSAASRFANYDDAEMRIKARGQTIGLGRNEGALVPANEGDLVEVTVLDRPVLFAPSDR
                     EQIFNGTVALAWQGTAEAAGYWLEIAGDVDFNAMKVSEWGLTDRTKTVERLDAGDYFW
                     RISALDAYGLPGERSLVRSFTAVLDATPPFLTVLAPGEDAILRDAAVRVSGESEPRAS
                     LTVNGAPVPPDETGRFEAHVEAVPGLNEVVLEAIDRAGNRTSKTRRFTYRADRLAALG
                     FDPSIPTDDAGHLLTRGPELGVSGSSTAEPGSRLIVRDATGEVVADALVAEDGRFAFT
                     VEADEAGAAYEVEVVAVSGAAEGGTGFTATRDALPPELSFGEPPPRATAVRWLQLWGS
                     AGDAVALAVNGTDLAITDGSFETLLSLVPGSNPIELVATDRVGNVAMQELAVVLDLDP
                     PEVGAAEVRRTGGADDGIEIVVAATDATDLKQAAAYTLSVDGEERSGYLRYDPESGLY
                     RETLPPAGGRLKLVEVTVEDYAGNATVRRFD"
     gene            6248..8692
                     /locus_tag="P5558_12200"
     CDS             6248..8692
                     /locus_tag="P5558_12200"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY25117.1"
                     /translation="MLITGAAGAIGTALVRALEADYRVVGLDLTGKEAACELIPVDLT
                     SADSVELALRKLADRHGRQIASVIHLAAYFDFSGEEHPAYDAVNVDGTRHLLRALQGF
                     EVEQFVYAGTMLVHRPGAPGVPISESTAIEPRWAYPKSKARAEDVIRAEHGAIPYLVL
                     RLAGLYDDRTAVPTLAEQIRRIYERDPKAHAYAGSLETGQAFLHQADMVEAFVRAVER
                     RRDLPADTAILIGEAEAAGYGELQQRLATLIHGEEDWTTLSIPKPLAAAGAWVEEKSE
                     PLVPDDLDQGEKPFVRPFMVEMADDHYELDISRARELLGWQPRHRILEVLPCIVENLQ
                     ADPVGWYEANGLTLPHWLAAARERTDDPDALRQRAEERRRDAHDRFRWAHFLNIGLGT
                     WLVTSPPILGYQSFWMTWSDVASGLLVMALGFLALSWRFALVRWAVAVVGLWVMLAPL
                     VFWAPTAAAYLNGTLVGALIFACAACARPAPGIGRVAVTTGPTIPPGWDYSPSDWFQR
                     LPIIILALLGLYVSRYLTAYQLGHIDGVWEPFFAGAAGDPRNGTEEIITSSVSRAWPV
                     PDAGLGALTYMLEILIGVIGSARRWRTMPWLVLVFGIMIVPLGAVSITFIIIQPIVLG
                     TWCTLCLITAAAMLIQIPYSVDELVATCQFLARRRRAGRPLLHVFLTGDTDDGPDESA
                     AADFERSPRAIIGDMLGGGITLPWTLAASMAVAVWLMFTRLTLGTEGVLADADHLIGA
                     LALTVAVTATAESVRAARFLNILLGMALVITVLVVDATWYQVAADLACGLALMALSVP
                     RGSVRSRYGSWSRFVV"
     gene            8711..9739
                     /locus_tag="P5558_12205"
     CDS             8711..9739
                     /locus_tag="P5558_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HRY25118.1"
                     /translation="MLAGLPLWANVLVFLVSAGLVWAAGTRLAYFADAIARRTGLGQA
                     AIGVLLLGGITSLPEIAVTGAASWGGDAALAVNNLLGGFAMQVVILAFADATFGRAAL
                     TSTIPNPIVLLQGVLGILLVALIAAGIGTGDMAFLGAGFWTWGIFGAFLYALRLVTAA
                     EGRPAWQVIGQPPAPELGEAEPEVERSARRLALSTAAAAAVIFVAGYALARASEALAE
                     QTGLGSSLFGAVFVAIATSLPEISTVLAAIRLGRHVMATSDILGTNLFDIALIFLVDL
                     IYAGDPVLNEVGAFSQVAAMLGITVTGVFVVGMIERRDPAIRGIGLDSLIVGAAYLGG
                     LGLLFHLR"
     gene            9829..10140
                     /locus_tag="P5558_12210"
     CDS             9829..10140
                     /locus_tag="P5558_12210"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY25119.1"
                     /translation="MALKAFREFPAGAKLQVESALTIAAFGRKADIAKPLKGFDEGVF
                     EIALRHRGDAFRVVYEVRIGADLWVVHAFQKKSRTGIKTPVQEIDLIRQRLKRLKETL
                     Q"
     gene            10137..10469
                     /locus_tag="P5558_12215"
     CDS             10137..10469
                     /locus_tag="P5558_12215"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY25120.1"
                     /translation="MTSHDDDLDLVRGSGNVFRDLGHADAEVLQLKAELAARIIGVLD
                     DRALTVRRAAELTGIAAADFSRLRRARLDRFTIDRLMIVLARLDQVVEVSVSVRPVER
                     AAETISLP"
     gene            complement(10513..11598)
                     /locus_tag="P5558_12220"
     CDS             complement(10513..11598)
                     /locus_tag="P5558_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRY25121.1"
                     /translation="MTTMRDRDTQDGEAPELPLNLRSDNVTGIAPPILDAILRANRGS
                     MASYGADERTLGLGERFSELFGKRCAVQPLGTGTATNALAMSLLTPPWGAIVCSAVSH
                     VHDSECGAAELFTGGAKVTPLPEADGKLAPETLERHLAKAGWGRTSEVQPAVLAIAQA
                     TERGTVYQPDEIAALAAIARRHGLKVHLDGARFANALAALGCAPADLTWKAGVDVLSF
                     GGTKNGCLAAEAIVMFDENLAEPLRFRARKAGHIFSKMRFVSAQLEAYVENGLWLRLA
                     AHANAMAARVATGLEEIEGARLLHPVDINEVFMALPDAAREGLKADGFGFYDRGAGTV
                     RLVTAFDTRPEQVDAFVASARRHAAGV"
     gene            complement(11595..12713)
                     /locus_tag="P5558_12225"
     CDS             complement(11595..12713)
                     /locus_tag="P5558_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY25122.1"
                     /translation="MRITDVEGIMLRLPEVREIGDGCQSVMIIKVHTDEGVTGIGEAH
                     TNPLVNKAVLDAPLCSISAQGLRGLLLGEDPRDIGRLWDRMDRHTQTYGRRGVAMHVI
                     SGIDIALWDILGKVAGLPVHRLLGGTRRTHHRAYASDLSQPSLEATVDLAKQHKANGY
                     KAMKFGWGDLGGDPRKDVRRTAAIRAAVGDEVDIMIDMGFAVPLDDALYLGRAFAEHG
                     VYFLEEPLAPDDLDGFARLTAVSPTPIATGEKEAGVHAYRMLMEHGGLRIIQPDIARV
                     GGISESLRIAALAESRGVRMIPHCWSTDILVAATLHVISTLRDCPYLEYNATDNPLRT
                     DLLVDPIRPVDGIVTVPEKPGLGIELNQDTIDRYRWNP"
     gene            12812..13990
                     /locus_tag="P5558_12230"
     CDS             12812..13990
                     /locus_tag="P5558_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY25123.1"
                     /translation="MTRLVIEGATITRLRVPLVKPYISSQVRRGRDLIERTVIALHGS
                     DGTTGYGETVGTAAVFAIVRDLCQRLRGHDAFAMGRFRRAWAPLTFSNMDGRNGFIAF
                     GGVEAACHDLVARHLGVPLADLLGGAQRTSIPCAGLMGALPVDEPMAEGEMAAFIADP
                     GNVAAVVAEAEGLVAESGYPSLKIKSVGLDQGWDLAVMTALRERFGPAMGLRLDVNGA
                     FSVAQALRLGRALEPLDLEYLEDPVSGIEGLARLRRDLRTPIATNMAIINMDQLPVGI
                     RMGAADVILGDIFHWGGIRNFCDLAAVARAFELSMGMHSFWEMGIATAFNLHLAAALP
                     VVDHPIDGILWLYPDDITKGPRLRVADGVLPLPDGPGIGVEPDPAKLGRWSVAEVKVG
                     "
     gene            14072..15646
                     /locus_tag="P5558_12235"
     CDS             14072..15646
                     /locus_tag="P5558_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25124.1"
                     /translation="MTRFAGSRHLLYGMAAAVLGTFTSFAVAAETFSCPNEGGDMVFG
                     QEAQVSGLDMHFSSAISTRNIAMHIYESLMTRAEDNSPIHELAASHEVSDDGLTYTFK
                     LRDGITFHNGKEMTSADVLASYERYQKIGIDRTSLEPVESMSAPDPSTFVIKLKQKVP
                     VFIEQISSFRVPIVILPAEEAAKEANQIEFIGTGPFEFVEWVPDGFVKLARFDDYAPN
                     AAFEGKTGFGGYKVACFDTVTFRIVKEPGARVAGLETGELQGVEDVPTKAAERLADNP
                     DIKLIPVDNWWIHIAVFNQASPPTDKLGVRKAIQVGLDMEEIMEIATDGAYTLNHGLQ
                     YPGNPYFVEGGKEWYNVNDKERAKQYLEEAGYNGEPVVLLTNSDYTNMYNAALVMSEQ
                     LKDIGINAELKVVDWPTSRQERDNTDNWNLFFTGFGTGPSIGPQGAVVDLVPPANLSR
                     APENPELNDAWSRMQLEETFEGRKAAFNDVQTIFYENAYAIVMGDIQKVQGVRSNVDG
                     FVPFRIPRLWNVWFEG"
     gene            15669..>16390
                     /locus_tag="P5558_12240"
     CDS             15669..>16390
                     /locus_tag="P5558_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25125.1"
                     /translation="MIRFIAGRLVSAVPVMLLVTLISFLLMKLVPGDPAMIIAGLSAT
                     PAEVEHIRQQLGLDQPFLTQLLTWYGNLLQGDLGQSLLLGRGVTTSILERLPVTLSIS
                     FYALFLTLVAALTSGTLAALRQNTWIDQILMSVALIGVSLPNFWLGLMLIILFAVWLG
                     WLPSGGYVPFFEDPWGWLAAATLPAVSLAMLQMGLLARITRATMLEVLRQDYIRTARA
                     KGMPPWIVVGKHAFKNVMIPVVT"
BASE COUNT         2335 a         5739 c         5657 g         2659 t
ORIGIN      
        1 gacccgtcgc cgaagttcca ggagaagagg ttgaccgcac cgtcggcatc ggtcgaatgc
       61 gagccgtcga agcggatggt ctggttggtg caggcccggg tcggtgctcc ggccaccgcg
      121 atcggcgcct cgcggaccac ggcggcgacc cggtcgctgt gcacgccgga agcgttgccg
      181 ctctcgtccc ggaccgtgag cgtcaccgga tagacgccgg gcagctcgta ggtcttggcg
      241 gggttgacga ggttggcccg ggtcccgtcg ccgaagtccc actcgtagcg caggaggccg
      301 ccatccggat cgctggaggc gctgccgtcg aagaggatgc tctcgcccga gcagacgtcg
      361 tgattgccgc cggccaccgc caccggccgg ctgtcgatca ccacgcgggt cgcatcgacg
      421 gcggtcgagt tggcgaggcc ggtgccgtcg tcgaccgtga gcgtcaccgg gaagacgccg
      481 ccgcgcgggt aggcatgggt gaccgtccgc ccggtcagcg cgggcgagcc gtcgcccggg
      541 tcccaggtga agaccagggt gtcgccgtcg gcgtcgaccg agccggagcc gtcgagggtc
      601 acgatgagct ggtcggtgcg gatctccgcc ccggcctcgg cgaccggcgc gtggttgacg
      661 tggatcagca tctcgtcgga gctggacgca ttgagcgtgc cgctgccgtc gctcacggtg
      721 agctgggccg tgtagatgcc cggctcgtcg aagacccgcc gtggcgagcg ctcgaggacc
      781 ggcgtcgccc gatcgtcgaa gtcccagcga tggacctcga tcgagccgtc cgggtcgaac
      841 gaggcagcgc tgctgaggcg aagctcggtg ccgggggcga tcagcacgtc cgggccggca
      901 tcggcgaccg gtgcggcgtt gatcgtcacg cgcgcctcgt cgacgtcgaa cgccgcctcc
      961 tggccgctgt cgtcccgcac cgtcagctcg acccggtagt ggccgggtgc gtcgaagctg
     1021 tgccgaattt gggccccctg gccgtcactg ccgtcgccga accgccaggc atagatgtcg
     1081 atcaccccgt ccggatcgag cgagccccgg ccatcgaagg tcagcgactg gcccggggcc
     1141 gccaccaggt ccgggccggc atcggcgatc ggcgcctggt tgacgcgcac catggcggtg
     1201 tcgcgatgga cgttgagcgg ggcgcccgaa tcgtcgcgga ccgtcagggc cacctcgtag
     1261 gtgccgggct cgacataggc atggcgcatg acccggccgg tgcccgtggc gccgtcgccg
     1321 aaggtccact cgtagctcgt gatcgtgccg tcggcgtcgc tggagccgcc gccgtcgaag
     1381 accacctcgc tggccgtgac gagctggtcc ggtcccgcgt cggccaccgg ggcggcgctc
     1441 acgaccacct ggaaggaggt ctccgccgtg tcggaccggg tcgccgagtt gtcgcgcgcg
     1501 gtcagccgca cctcgtagat gccgggtgcg ccataggcgt attcgacaac cgccccctcg
     1561 gcgatggcgc cgtcgccgaa cgcccactgg tagtccagga tctcgccgtc cggatcggtc
     1621 gattgcgtgg catcgaagcg ggcgatctcg ccgaccgcga tcggccgcgt cggctgcgtc
     1681 gagactggca ccggcggtgc attgaccgtg atccgcacgg tgtcggaggt ggagctgttg
     1741 cccacgccgg catcgtccac caccgtcagc cgcgcctcgt agacgcccgg cgcctggaag
     1801 gcatggtcgg cggcggccgt gctggcactg tcgccatcgc cgaaggccca gtgccacccg
     1861 gcgatctcgc cgtcgacatc gtagctggca cctccatcga agcggatggc ctcgccgacc
     1921 gcggcggaac gcgcctcgcc ggcttcggcc gtgggctgga agttgatggt gacgagcctt
     1981 tgggcgatgc cgaaattgca gggatgatcg gcgtcgtcgg cgacgcgcag cacgctgcgg
     2041 tagagacccg gcgtcgcata ggtcttgcgt gcttcggcac cgctcgcctg gctgccgtcg
     2101 ccgaagctcc agaggaagct cgagatcggc cggtcgctcg gcagcgaggc cgagccgtcg
     2161 aacaggatcg cgtcgccggg ggcggcgacg accgcttcgc ccgccaccgc cgtcggccgg
     2221 ttgcgcacat ggaccggcag ccggatcgag ctgccggaag cgacgcggtc cgagggaccc
     2281 agcacctgca gctcggcctc gtagcgaccg ggaatgtcgt agcggtggac caccacggtt
     2341 tcggccgagt tccggccgtc gccgaagagc cactgcaggc gcagcgggtg gtcgccgctc
     2401 gagccgctgg cgtcgaacgc gaccgcccgg caatcggcga gcggtgttgc gatcccgacc
     2461 gcctccggcc gcagggccgg ctgccggagc cgcgccggca gctcgaaggg caggggcccg
     2521 ccggcttcgt cgaagagggc gaaggtgatg tcgttcggcg tctcgttgcc gcccttgaac
     2581 tcgattgccg tctcgccctc gggcacggtg aaggtgcccg acgcccaggc atcctggccg
     2641 gatgccggca gggccgtgct cgcgaaggtc gaaaccagct cgaccgaggc ttccgcacca
     2701 tcgaagttct gcaaggtggc ccgctcgccg gccggggccg aaaaacgcag ctccaccacc
     2761 tcgcgcgttc cgggccagcg gagcgtcggg cgaaaggcga agagccggac gtcggccggc
     2821 agctcgctcg cgtccggtgc ccggctgacc tcgaaggcga agacattgcc gtcgtcaccg
     2881 gcttcgccga tcacatcgag gcggaaatag gcgcggccgt cccggatctc gccctggctc
     2941 gcctggaagg gggtgagcgt cacccagcgg tcgtcggtgg cgggctctgc cccgaagcgc
     3001 tgctcggcga tcagaacacc cgcctcgtcc ggcagcggca gcccagcctc cggatcggcc
     3061 ggcgagccat cggccaccgg tggcgggaac tcgacaccgg tgtaagcgcc cgcgccgccc
     3121 aggaggcgaa acagcacggt gctgttgccg aagctgccat aggccgtgtc gtgcaggccg
     3181 ttggcctcgg ggtcgaagag gcggaggaag aggtggccct cgaggtcggc gggcacgctg
     3241 atgaacaggt tctcgcggtg atcgaggtca ccttcgcgcg tctgcgccac ggggccatag
     3301 acgacgttca gcggcaccgt ctgcgctgac gcccgctcgg ccggcgacaa gccgccgagc
     3361 gccatgccga gggccagtgc ggcgagacct ttccgctgcc acctgatcat cgttcgcccc
     3421 ccgtcctgca cggcccggtt gctcatattg ctgcggcttg ccgggcgatt gacatcaatc
     3481 gaagcgtcgc acggtggcat tgcccgcgta gtcctcgacc gtgacctcga ccagcttgag
     3541 gcggcccccg gcgggcggca aggtttcgcg gtagagaccc gactccgggt cgtacctcag
     3601 atacccgctg cgctcctcgc catcgaccga aagggtgtac gcggccgcct gcttgaggtc
     3661 cgtggcgtcg gtggcggcga ccacgatctc gattccgtcg tcggcaccgc cggtgcggcg
     3721 cacctcggcg gcccccacct cgggcgggtc gagatcgagc accaccgcca gctcctgcat
     3781 ggcgacgttg ccgacccggt cggtggcgac gagctcgatc gggttcgacc cgggcaccag
     3841 cgacagcagg gtctcgaagc tgccgtcggt gatcgccagg tcggtgccgt tgacggcgag
     3901 ggcgaccgcg tcgccggcac tgccccagag ctgcagccag cgcacggccg tcgcccgggg
     3961 cggcggctcg ccgaagctca gctccggcgg cagcgcgtcc cgcgtggcgg tgaagccggt
     4021 cccgccctcg gcggccccgc tcaccgcgac cacctcgact tcgtaggcgg cgccggcctc
     4081 gtccgcctcg acggtgaagg cgaatcggcc gtcctcggcc accagcgcgt cggcgacgac
     4141 ctcacccgtg gcatcccgga cgatcaggcg cgagccgggc tcggcggtcg agctgccgct
     4201 gacgccgagc tcggggccgc gcgtcagcag atgtcccgca tcgtcggtcg ggatcgacgg
     4261 gtcgaagccg agtgcggcca ggcgatccgc acggtaggtg aagcggcgcg tcttgctcgt
     4321 ccggttgccg gcccggtcga tggcctccag caccacctcg ttgagccccg gcacggcttc
     4381 gacatgcgcc tcgaaccggc cggtctcgtc gggcggcacg ggcgcgccat tgaccgtcag
     4441 gctggcccgg ggctcgctct cgcccgacac ccggaccgcg gcgtcgcgga gaatggcgtc
     4501 ctcgcccggg gccagcacgg tgaggaaggg cggcgtcgcg tcgaggacgg ccgtgaagct
     4561 ccgcaccagg ctgcgctcgc ccggcaggcc gtaggcgtcg agcgccgaga tgcgccagaa
     4621 atagtcaccg gcatcgagcc gctcgaccgt cttcgtcctg tcggtcaggc cccactccga
     4681 gaccttcatg gcgttgaagt cgacgtcgcc cgcgatctcg agccagtagc cggcggcctc
     4741 ggccgtgccc tgccatgcga gggcaacggt gccgttgaag atctgctcgc ggtcggatgg
     4801 cgcgaacagc accggccggt cgagcaccgt cacctcgacc agatcgccct cgttcgccgg
     4861 caccagcgca ccctcgttcc tgccgaggcc gatggtctgg ccgcgcgcct tgatgcgcat
     4921 ctcggcgtcg tcgtaattgg cgaagcgcga ggccgcgctg tcgtggctca cccagaaatc
     4981 ggccgaggtc gcctcggtct cgacacccgg cacctcgacc tcgaagtcgg cccggttgtg
     5041 cagctggttg agcagcgcgt agaaatcgcc ctcgaccagg ctgaccttgg tggtctcggc
     5101 cccggtcagc gggtcgctgc gcatgcgctg gatgacggcg ttggagttcg ggttgaggcg
     5161 cagccggctg agatcgcgga aggtcacctg ggcggtcgag gcggagagcg tgcgcagccg
     5221 ctcgaactcg atcaggaaat cgttcagccg gcggcccgac cagccggtct cgacaggctt
     5281 gctgccctcg acagcgccgt gaatgtcgct gagcacggcc tcggccgccc ggtcgcgctg
     5341 ctgcagcgag atatcgagcg cctgctcggc atgggcggtc gcctcgttgg cgtggaccac
     5401 gacctcgcgc cattcgcccg cggtccggta ttccaccgcc tcgtcgcgct tctcgatcgc
     5461 cgtgccgatc tcgatcgggg cgaagatccg cgccccctcc gcagtcgcct tctggatcga
     5521 cacgagcgag atggcgaggg cgtcatccgc ggcggcgacc tggacgaccg ggaccttgag
     5581 ctcgatgccc gggcggacgt cgacgaccga gccgaggccg ctgaactcga tgatctgcgg
     5641 ccagagatcg gggtccttca ggtagagggc ggcgaggtcg cgtatgctct gcccctgctc
     5701 gacgcgtacc gtggtgaggg ggccgtcgat ctcgacgacg gcgagcgccg ccggcgcgcc
     5761 gagaagcagg gcggcgccga tccaccgcat cgcccagccg gccaatctcg atcgaccgtt
     5821 tcccccgccg acagtcatgc gatctgtctt ttcttgtgct gacgccgcag gatacgcgaa
     5881 tcgtcgagca ccgcaaggcc ggacaggcgc ccgatcgctc gccgagtcat gccgcatact
     5941 tatctacggc caagattcgg catttgcttt tcttgagaag atggccgtcg gccgcagagc
     6001 gcggccgggc gaggggcggt gacgggatgg ctcgcacctt gcggcacctc cgggctgctg
     6061 gaaccggagc cgggccgagc ccgttgccca accgaagcca tggcggctcc gccgccggcg
     6121 gctcgccacg agccgcgagc tcggcgccgc cgcgcctgct gcagcccact tccgggaggg
     6181 acaggagcga ccattgccag ttgcaccacc gaacgcaggg agcgacaccg cgtcggccag
     6241 gcctctggtg ctcatcacgg gcgctgccgg cgccatcggc accgccctcg tgcgggcgct
     6301 cgaagccgat tatcgggtcg tcggcctgga cctcaccggc aaggaagccg cctgcgagct
     6361 catcccggtc gatctcacga gcgccgattc ggtcgagctg gccctgcgca agctcgccga
     6421 ccgccatggc cgccagatag cctcggtcat ccatctcgcc gcctacttcg atttctccgg
     6481 cgaggagcat ccggcctacg acgccgtcaa tgtcgacgga acgcgccacc tcctgcgcgc
     6541 tcttcagggc ttcgaggtcg agcagttcgt ttatgccggc accatgctcg tgcatcgccc
     6601 gggggcgccc ggggtgccga tcagcgaatc gacggccatc gagccgcgct gggcctatcc
     6661 gaagtccaag gcccgggccg aggacgtgat tcgcgcggag cacggcgcca tcccctatct
     6721 cgtcctgcgc ctggccggcc tctacgacga ccggaccgcg gtgccgaccc tggccgagca
     6781 gatccgccgc atctacgagc gcgaccccaa ggcccatgcc tatgccggct cgctcgagac
     6841 gggccaggcg ttcctccacc aggcggacat ggtcgaggcg ttcgtgcgcg ccgtggagcg
     6901 gcgccgggat ctcccggcag acacggccat cctgatcggc gaggcggagg ccgccggcta
     6961 tggcgagctg cagcagcgcc tggcgacgct catccatggc gaggaggact ggaccacgct
     7021 ttcgatcccc aagcccctgg ccgctgccgg ggcctgggtc gaggagaaga gcgagccgct
     7081 cgtgccggac gacctggacc agggcgagaa gcctttcgtc cgaccgttca tggtcgagat
     7141 ggccgacgac cactacgagc tcgacatctc ccgtgcccgg gagctgctcg gctggcagcc
     7201 acggcatcgc atcctcgagg tgctgccgtg catcgtcgag aacctccagg ccgaccctgt
     7261 cggctggtac gaggccaacg gcctgacgct gccgcactgg ctggcggcgg cgcgcgagcg
     7321 tacggacgat ccggatgcgc tgcgccagcg ggccgaggag cgccggcgcg acgcccacga
     7381 tcgctttcgc tgggcacatt ttctcaatat cggcctcggc acctggctgg tcaccagccc
     7441 gcccatcctc ggctaccagt ctttctggat gacgtggagc gacgtcgcca gcgggctcct
     7501 ggtcatggcc ctgggctttc tcgccctatc ctggcgcttc gccctggtcc gctgggcggt
     7561 ggcggtggtc gggctctggg tgatgctcgc cccgctcgtg ttctgggcgc cgacggccgc
     7621 cgcctatctg aacgggacgc tggtcggcgc cctgatcttc gcctgcgccg cctgcgcaag
     7681 accggcgccc gggatcggcc gcgtcgccgt caccaccggg cccaccatcc cgccgggctg
     7741 ggactactcg ccgtccgact ggttccagcg cctgccgatc atcatcctgg cgctgctcgg
     7801 cctatacgtc tcgcgctacc tgaccgccta ccagctcggc catatcgacg gcgtctggga
     7861 gcccttcttc gccggcgccg ccggcgaccc caggaacggc accgaggaga tcatcacctc
     7921 ctcggtctcg cgcgcctggc cggtgccgga tgccggcctc ggcgcgctca cctacatgct
     7981 ggagatcctg atcggcgtca tcggctcggc gcggcgctgg cgcaccatgc cctggctggt
     8041 cctggtcttc ggcatcatga tcgtgccgct cggcgcggtc tcgatcacct tcatcatcat
     8101 ccagccgatc gtgctcggca cctggtgcac gctctgcctg atcaccgcgg cagccatgct
     8161 gatccagatt ccctattcgg tcgacgagct ggtcgccacc tgtcagttcc tggcccgacg
     8221 gcggcgcgcc ggccggccgc tgctgcatgt ctttctcacc ggcgacaccg acgatggccc
     8281 cgacgagtcc gcggccgccg acttcgagcg ttcgcccagg gccatcatcg gcgacatgct
     8341 cggtggcggc atcacgcttc cctggacctt ggcagcctcc atggctgtcg cggtctggct
     8401 gatgttcacc cggctcacgc tcggcacgga gggcgtgctc gccgatgccg accacctgat
     8461 cggcgccctg gcgctcaccg tcgccgtcac ggcgaccgcc gagtccgtgc gggcagcgcg
     8521 cttcttgaac atcctgctcg gcatggcgct cgtcatcacg gtgctcgtcg tcgatgccac
     8581 ttggtaccag gtcgccgccg acctcgcctg cgggctggcc ctgatggcgc tcagcgtgcc
     8641 gcgcggttcg gtgcggagcc gctacggcag ctggtcgcgc tttgtcgtct gagcgggcag
     8701 ggagggggcc gtgctcgctg gcctgccgct ctgggccaac gtcctcgtct tcctcgtctc
     8761 ggccggtctt gtctgggccg ccggcacgcg gctcgcctat ttcgccgacg ccattgcgcg
     8821 ccgcaccggc ctcggccagg cggcgatcgg cgtccttctg ctcggcggca tcacctcctt
     8881 gccggagatc gcggtcaccg gcgccgcatc ctggggtggt gacgccgcgc tggcggtcaa
     8941 caacctcctc ggcggcttcg ccatgcaggt ggtgatcctc gccttcgccg atgccacctt
     9001 cggccgagcg gcgctcacct cgaccattcc gaacccgatc gtgctgctcc agggcgttct
     9061 cggcatcctg ctggtggcgc tgatcgctgc cggcatcggc accggcgaca tggccttcct
     9121 cggcgcgggc ttctggacct ggggcatttt cggcgccttc ctctacgcgc ttcgcctcgt
     9181 caccgccgcc gagggccgcc ctgcctggca ggtgatcggt cagccgcccg cacccgagct
     9241 cggcgaggcg gagcccgagg tcgagcgctc ggctcggcgc ctggcgctat cgacggctgc
     9301 cgccgccgcc gtcatcttcg tggccggcta cgccctcgcc cgcgccagcg aggcgctggc
     9361 cgagcagacc ggcctcggct cgagcttgtt cggtgccgtc ttcgtcgcca tcgcgacctc
     9421 gctgcccgag atctcgaccg tgctcgccgc cattcgcctc ggccgccacg tgatggcgac
     9481 ctccgacatc ctcggcacca acctcttcga catcgccctg atcttcctcg tcgacctgat
     9541 ctacgccggc gacccggtgc tcaacgaggt cggcgccttc tcgcaggtgg cggccatgct
     9601 cggcatcacc gtgaccggcg tcttcgtcgt cggcatgatc gagcgccgcg acccggccat
     9661 ccgcggcatc ggcctggatt cgctgatcgt cggggcggcc tatctgggcg gcctcgggct
     9721 cctcttccac ctgcgctgag ggtgtggacg ggggagggtg cttgaccatg ttgtcagttt
     9781 tgactacatg acctcatgga ttcgccccgg ccgatctcgt gggtcaaggt ggcgctcaag
     9841 gcgttccgcg aatttccggc tggggcaaag ttgcaggtcg agtccgccct gacgatcgct
     9901 gccttcggcc gcaaggcgga cattgccaag ccattgaagg gcttcgacga aggcgtgttc
     9961 gagatcgccc ttcggcatcg tggcgacgcc tttcgtgtcg tctatgaggt gcggatcggc
    10021 gccgatctgt gggttgtcca tgcctttcaa aagaagtccc ggacaggcat caagacgccg
    10081 gtccaggaga tcgacctgat ccgccagcgg ctcaagcgcc tcaaggagac tctccagtga
    10141 ccagccacga tgatgatctc gacctcgttc gcggcagcgg caatgtcttc cgcgacctcg
    10201 gccacgccga cgccgaggtc ctgcaattga aggccgagct ggcggcgcgg atcatcggcg
    10261 tgctcgacga ccgcgcgctc accgtccgtc gcgccgccga gctgaccggc atcgcggcgg
    10321 ccgacttctc ccgcctccgc cgcgcccgcc tcgaccgctt caccatcgac cgcctgatga
    10381 tcgtcctggc gcggctcgac caggtggtcg aggtgagcgt cagcgtgcga ccggtggagc
    10441 gagcggcgga aacgatctca ttgccttgaa gcgacgacgc tggcaggccg ttcctccgaa
    10501 cgcggcgaac cgttagacgc ccgccgcatg cctgcgcgcc gaggcgacga acgcatcgac
    10561 ctgctccggc ctcgtgtcga acgccgtcac caggcggacc gtgccggcgc cgcggtcgta
    10621 gaatccgaag ccgtccgcct tcaggccctc gcgggcggca tccggcaggg ccatgaagac
    10681 ctcgttgatg tcgaccgggt ggagcagcct cgccccctcg atctcctcga ggcccgtcgc
    10741 caccctggcc gccatggcgt tggcgtgggc tgccaggcgg agccagaggc cgttctcgac
    10801 ataggcttcg agctgggccg agacgaagcg catcttggag aagatgtggc cggccttgcg
    10861 ggcgcgaaag cgcagcggct cggccaggtt ctcgtcgaac atgacgatcg cctcggcggc
    10921 gagacagccg ttcttggtgc cgccgaagga gaggacgtcc acgcctgcct tccaggtcag
    10981 gtcggcgggc gcgcagccga gggcggccag cgcattggcg aagcgggcgc cgtcgagatg
    11041 caccttgagc ccgtggcggc gggcgatggc ggcgagggcc gcgatctcgt ccggctggta
    11101 gacggtgccg cgctcggtcg cctgggcgat ggcgagcacg gccggctgga cctcgctggt
    11161 ccgcccccag ccggccttcg ccagatgccg ctcgagcgtc tcgggtgcga gcttgccatc
    11221 ggcctcgggc aggggtgtga ccttcgcccc gccggtgaag agctcggccg cgccgcattc
    11281 gctgtcgtgg acgtggctga cagcgctgca gacgatggcc ccccaaggcg gggtcaggag
    11341 cgacatggcg agcgcattgg tcgcggtccc ggtgccgagc ggctgcacgg cgcagcgctt
    11401 gccgaagagt tcggagaagc gctcgccgag acccagcgtc cgctcgtccg ccccgtagga
    11461 ggccatgctg ccccggttgg cccgcaggat ggcatcgagg atcggcgggg cgatccccgt
    11521 cacgttgtcg ctgcgcaggt tgaggggcaa ttcgggggct tcgccgtcct gtgtgtcgcg
    11581 gtcgcgcatg gtcgtcatgg gttccacctg tagcggtcga tcgtgtcctg gttgagctcg
    11641 atgccgaggc ccggcttttc cggcacggtg acgatgccgt ccaccggccg gatggggtcg
    11701 acgaggaggt cggtgcgcag cgggttgtcg gtggcgttgt attccagata ggggcagtcg
    11761 cgcagcgtcg agatgacgtg cagcgtggcg gcgacgagga tgtcggtcga ccagcagtgc
    11821 gggatcatcc gcacgccgcg cgattcggcc agggcggcga tgcgcaggct ctcggagatg
    11881 ccgccgaccc gggcgatgtc gggctggatg atgcgcaggc cgccatgctc catgagcatg
    11941 cggtaggcat gcacgccggc ctccttctcg cccgtggcga tcggtgtggg cgagaccgcg
    12001 gtgagccggg cgaagccgtc gaggtcgtcc ggggccagcg gctcctccag gaagtagacg
    12061 ccgtgctcgg cgaaggcccg gccgagatag agcgcgtcgt cgagcggcac ggcgaagccc
    12121 atgtcgatca tgatgtcgac ctcgtcgccg accgcggccc ggatggcggc ggtgcgcctg
    12181 acgtcctttc ggggatcgcc gccgagatcg ccccagccga acttcatggc cttgtagccg
    12241 ttggccttgt gctgcttcgc gagatcgacc gtggcctcga gcgagggctg cgagaggtcg
    12301 ctggcatagg cccggtgatg cgtcctccgc gtgccgccca gcaggcggtg aaccggcagc
    12361 cccgcgacct tgccgaggat gtcccaaagg gcgatgtcga tgccgctgat gacgtgcatg
    12421 gccacgccgc gccggccgta ggtctgggtg tggcggtcca tcctgtccca gagccggccg
    12481 atgtcgcgcg ggtcctcgcc gagcagcagc ccgcgcaggc cctgggcgga gatggagcaa
    12541 aggggggcgt cgaggacggc cttgttgacc agcgggttgg tgtgcgcctc gccgatgccg
    12601 gtgacgccct cgtcggtgtg gaccttgatg atcatcaccg actggcagcc gtcgccgatc
    12661 tcccggacct ccggcagccg cagcatgatc ccttcgacgt cggtgatgcg catgcggtct
    12721 ttttcctttc cggatttatc ctgacatgtc aggaaggttt ggccatgcga tgcgcgaatg
    12781 acaagcccgc cttgcgacga cggccccgcc gatgacccgg ctggtgatcg aaggggcgac
    12841 gatcacgcgg ctcagggtgc cgctggtcaa gccctacatc tcctcccagg tccgccgcgg
    12901 ccgcgacctg atcgagcgga cggtgatcgc gctccacggc agcgacggca ccaccggcta
    12961 cggcgagacc gtcggcacgg cagcagtctt cgccatcgtc cgggatctct gccagcggct
    13021 gcgcggccac gacgccttcg ccatgggccg gtttcgccgg gcctgggcgc cgctcacctt
    13081 ctccaacatg gatggccgca acggcttcat cgccttcggc ggggtcgagg ccgcctgcca
    13141 cgacctcgtg gcccgccacc tcggggtgcc gctggccgac ctcctgggtg gtgcccagcg
    13201 cacgtccata ccctgcgccg gcctcatggg cgcgctgccg gtcgacgagc ccatggccga
    13261 gggcgagatg gcagccttca tcgccgatcc tggcaatgtc gcggccgtgg tggcggaggc
    13321 cgagggtctc gtggccgaga gcggctatcc cagcctcaag atcaagagcg tcgggctcga
    13381 ccagggctgg gacctcgccg tgatgaccgc cctgcgcgag cgcttcgggc cggcgatggg
    13441 cctcaggctg gacgtcaacg gcgccttcag cgtggcccag gcgttgcgcc tcggccgtgc
    13501 cctggagccg ctggacctcg aatatctcga ggacccggtc agcggcatcg aggggctcgc
    13561 acggctgcgg cgcgatctgc gcacgccgat cgccaccaac atggcgatca tcaacatgga
    13621 ccagctcccc gtcggcatcc gcatgggtgc ggccgacgtg atcctcggcg acatcttcca
    13681 ctggggcggc atccgcaatt tctgcgatct cgccgccgtg gcgcgggcct tcgagctgtc
    13741 gatgggcatg cattccttct gggagatggg catcgccacg gccttcaacc tgcatctggc
    13801 ggcggccctg ccggtggtcg accatccgat cgacggcatc ctctggctct atccggacga
    13861 catcacgaag ggaccccggc tgcgcgtcgc cgatggcgtg ctgccgctgc cggacggccc
    13921 cggcatcggt gtcgagccgg acccggcgaa gctcggccgg tggtcggtcg cggaggtgaa
    13981 agtggggtga ctttggtcgg cccgccatgc aggatgttgg tcaatgaagg gagcctgcct
    14041 gccgaggtgg gccaagaaag ggaggcgagg aatgacgaga ttcgcaggtt ccaggcattt
    14101 gctgtacgga atggcggcgg cggtgctggg caccttcacg agttttgccg tcgcggcgga
    14161 aaccttctcc tgcccgaatg agggcggcga catggtgttc gggcaggagg cgcaggtctc
    14221 gggcctcgac atgcatttca gctcggcgat atcgacgcgc aacatcgcca tgcacatcta
    14281 cgaatcgctg atgacgcgcg ccgaggacaa ctcgccgatc cacgagctgg cggcgagcca
    14341 cgaggtcagc gacgacggcc tgacctacac cttcaagctc agggatggca tcaccttcca
    14401 caacgggaag gagatgacgt cggccgacgt gctcgcctct tacgagcgct accagaagat
    14461 cggcatcgac cgcacctcgc tcgagccggt cgagagcatg agcgcacccg atccctccac
    14521 cttcgtcatc aagctgaagc agaaggtgcc ggtcttcatc gagcagatca gctcgttccg
    14581 cgtgccgatc gtcatcctgc cggccgagga ggcggcgaag gaagccaacc agatcgagtt
    14641 cattggcacc ggccccttcg agttcgtcga gtgggtgccg gatggcttcg tcaagctggc
    14701 tcggttcgac gactacgcgc ccaacgcggc attcgagggc aagaccggct tcggcggcta
    14761 caaggtcgcc tgcttcgaca ccgtgacctt ccgcatcgtc aaggagccgg gggcgcgcgt
    14821 cgccgggctc gagacgggcg agctgcaggg cgtggaggac gtgccgacca aggcggccga
    14881 gcggctcgcc gacaacccgg acatcaagct gatcccggtc gacaattggt ggatccacat
    14941 cgccgtcttc aaccaggcga gcccgcccac cgacaagctg ggcgtgcgca aggcgatcca
    15001 ggtcggcctc gacatggagg agatcatgga gatcgccacg gacggggcct acacgctcaa
    15061 ccacggcctc cagtacccgg gcaatcccta cttcgtcgag ggcggcaagg agtggtacaa
    15121 cgtcaacgac aaggagcggg cgaagcagta tctcgaggaa gcgggctaca atggtgagcc
    15181 ggtcgtcctc ctgaccaatt ccgactacac caacatgtac aatgccgccc tggtgatgtc
    15241 cgagcagctc aaggacatcg gcatcaatgc cgagctcaag gtggtcgact ggccgacctc
    15301 gcggcaggaa cgcgacaaca ccgacaactg gaacctgttc ttcaccggct tcggcaccgg
    15361 cccgtcgatc ggcccgcagg gtgcggtcgt cgacctcgtg ccgccggcca acctgtcgcg
    15421 ggcacccgag aatcccgagt tgaacgatgc ctggtcgcgc atgcagctcg aggagacctt
    15481 cgagggccgc aaggccgcct tcaacgatgt ccagaccatc ttctacgaga atgcctatgc
    15541 gatcgtgatg ggcgacatcc agaaggtcca gggcgtgcgc agcaatgtcg acggcttcgt
    15601 gccgttccgc atcccgcggc tgtggaacgt ctggttcgaa ggctgaatca ggcagtgggg
    15661 ccggcgatat gatccgtttc atcgccggcc gcctcgtcag cgccgtgccg gtcatgctgc
    15721 tggtcacgct gatctcgttc ctcctgatga agctggtccc gggtgatcct gcgatgatca
    15781 tcgccgggct ctcggcaacg ccggccgagg tcgagcacat tcgccagcag ctcggcctcg
    15841 accagccctt cctgacccag ctcctgacct ggtacggcaa cctcctgcag ggcgatctgg
    15901 gccagagcct gctgctcggc cggggggtca ccacctcgat cctcgagcgc ctgccggtga
    15961 ccctctcgat ctccttctac gccctcttcc tgacgctggt cgcggccctc accagcggca
    16021 ccctggccgc ccttcgccag aacacctgga tcgaccagat cctgatgagc gtggcgctca
    16081 tcggcgtgtc cctgccgaac ttctggctcg ggctgatgct catcatcctc ttcgccgtct
    16141 ggctcggctg gctgccgtcg ggcggctatg tgcctttttt cgaggatccc tggggctggc
    16201 tcgccgccgc caccctgccg gccgtctcgc tcgccatgct gcagatgggg ctgctcgccc
    16261 gcatcacccg ggcgaccatg ctcgaggtgc tgcggcagga ttacatccgc acggccaggg
    16321 cgaagggcat gccgccctgg atcgtcgtcg gcaagcatgc cttcaagaac gtgatgatcc
    16381 cggtcgtcac
//
LOCUS       DAOTFI010000107        16232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4320_length_16232_cov_6.960809, whole genome shotgun sequence.
ACCESSION   DAOTFI010000107 DAOTFI010000000
VERSION     DAOTFI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 16232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16232
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4320_length_16232_cov_6.960809"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="P5558_12245"
     CDS             <1..222
                     /locus_tag="P5558_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25126.1"
                     /translation="DSMNSPNMILDLRIPQNQKYQQVVLDEAISRFLAGEIDKEATVQ
                     AVANGWNELNDEIGVDEQLEIYRATIGAN"
     gene            304..1443
                     /locus_tag="P5558_12250"
     CDS             304..1443
                     /locus_tag="P5558_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25127.1"
                     /translation="MTAFAETAVDTVPRPGWGERLDRHAGRLMVLPAVVILLCFAIFP
                     LIISAYLALSRFALAPGGFTLKFIGLGNFRKLLTGSQQYHFLGTFGAFGPVAWGLVAL
                     AAIALLYCMARYLRGGSVTVVGIVGRLISVSLTMGLVLVAVATLATSGVPGTLVTTLL
                     YVVIGVSVQFLLGLGLALLCAQPIRGRNFFRVLYFVPLMVTPVGIAYTFRMLADMQKG
                     PFAPLVRSFGVGEWSWATEAWSARLMVLVGDTWQWTPFMFIVLLAAIENQPRDQVEAA
                     RIDGAGGLQIFRDITWPAIAPVAGTVVLIRLIEAFKIIDLPNVLTGGGPGLATESMTL
                     HAFIAWRTQDLGASAAVGYMLLFVSTVVCITFFNFVVRPSRRLES"
     gene            1440..2486
                     /locus_tag="P5558_12255"
     CDS             1440..2486
                     /locus_tag="P5558_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25128.1"
                     /translation="MNGDRRSLRQRLFEPESLERQAPVPKFMTYVLLVFWSLVVLVPL
                     YWVLITSFKQSGDVDNGPFYIPFVDFTPSLHAWDFMLLQNNTLGPYINSIVAALGSTV
                     VGVVVGALAAYALVRTRLEVKLATVVTFLLLLAATVAAVVTLGVPWPIALGSAVVIFI
                     LAVFTLTHRARLALGNRDIEFWIISNRIMPPIVAVLPIYIMFQQLRLLDTQLALVATY
                     AAVNLPIVVWLTRDFFAAIPVDLEESAEIDGASKFRVFFTIALPLVRSGLVATFLLVL
                     ILAWNEYLLALFLTNANAQTMPVLVSAQNTTRGPQWWNMSVLIVVMIAPVVVIATVLQ
                     KHIARGLLVGAVKG"
     gene            2494..3552
                     /locus_tag="P5558_12260"
     CDS             2494..3552
                     /locus_tag="P5558_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25129.1"
                     /translation="MRVTLSNIFKSFGAVQVVKGLDLEIEDGEFLVLLGPSGCGKTTA
                     LRMVAGLESVTSGRIAIGERDVTQVLPKYRDVAMVFQSYALYPHMTVAKNIGYPLKLR
                     GVPKAEQETQIRAAAEKVHLEDYLDRFPRQLSGGQRQRVALARAIVRRPSVFLMDEPL
                     SNLDAKLRSYMRAELKHLQEELGITTIYVTHDQIEAMTLAHRVAVMNQGVVQQIATPR
                     EIYDNPANLFVAGFIGSPPMNTLTGPIEDGSFVLPEGRVAVGNYAPRSEAVFGFRPED
                     AQIVSPDAADLQARVFACELTGNETIVTCEVGKAPVVVKMDKNYDVRAGTAVGIAIDR
                     AKLCLFDAGTGEKIAAGG"
     gene            complement(3574..4335)
                     /locus_tag="P5558_12265"
     CDS             complement(3574..4335)
                     /locus_tag="P5558_12265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY25130.1"
                     /translation="MSFARYPSLDDVAVLVTGGASGIGEQIVRAFAEQGSRVGFVDLD
                     TDRGTALAGELRDAGAKVHFETCDLRDIEALKRAFAAVEAAQGPTTVLVNNAARDDRH
                     GWQDVTPEYFDERIATNLRHMFFAIQAVAPGMIKAGKGSIINFGSNSWWEAGGGFPVY
                     TQAKAAVHGLTRGMARDLGQHRIRVNTVVPGWIMTERQKTLWATPEALEKHRQRQCLP
                     DLIQPVYLARMVLFLASDDAAMCTANNYMVEAGSI"
     gene            complement(4428..4925)
                     /locus_tag="P5558_12270"
     CDS             complement(4428..4925)
                     /locus_tag="P5558_12270"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRY25131.1"
                     /translation="MPTNRSTKAGLALSFMALLGAVPAVAEEAATDALAETPQVDVAP
                     GAAELPPTTGAVSVTSLLELDVVSADDEELGDVYDLVADPEDGLLKFLVIQRGGEILG
                     IDIGVGAERVAVPWHRVEMQEAPRRLIVDFTAKEIEALPDWEGARTEGGLVGAAPVDE
                     PKGVN"
     gene            complement(5001..5777)
                     /gene="mazG"
                     /locus_tag="P5558_12275"
     CDS             complement(5001..5777)
                     /gene="mazG"
                     /locus_tag="P5558_12275"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRY25132.1"
                     /translation="MSAERPIDRLLAVMRRLRDPEIGCSWDIEQTFATIAPYTIEEAY
                     EVADAIERADWEELRGELGDLLLQVVYHAQMAAEAGLFGFDDVATAIADKMIARHPHV
                     FGDVEVKGAEAQHEHWESAKAAERQAKAASDGSAASLLDNLPHGLPALTRALKLQKRV
                     LRVGFDWPDIQGVRDKVNEELDELESAAPDERELELGDLLFTLVNHARRLGLDPETAL
                     RRANAKFESRFRRMERTSDRPLGELDLEALEALWQRVKTG"
     gene            complement(5774..7171)
                     /gene="xseA"
                     /locus_tag="P5558_12280"
     CDS             complement(5774..7171)
                     /gene="xseA"
                     /locus_tag="P5558_12280"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRY25133.1"
                     /translation="MVSNLHEYSVSEIAFALKKTIEDAYGLVRVRGEISGFKRASSGH
                     LYLALKDDKAVLDGVCWRGTAGALGFKPEDGLEVIATGRLTTYPGRSRYQLVIEHMEP
                     AGVGALMALFEERKQRLAAEGLFEPERKRPLPYLPRCIGLVTSPTGAVLRDILHRLAE
                     RFPLSVLLWPVAVQGEGAAEQIAAAIGGFDRLAAEGAIPPPDLLIVARGGGSIEDLWA
                     FNEEAVARAVAACSIPLISAVGHETDTTLIDFVADRRAPTPTAAAEMAVPVRAELLAS
                     TEGLAERLIHALERGLRDRRQGLDGLARGLPDPLAILRFAEQRLDDLTERLRLRTPLA
                     VLDDQAEKLGGRHARLGELVLDRVRQAERDLARLPAETLDQRIADRLRELQRSLDGPA
                     KLLESLSHRRVLDRGYALVHGASGGLVTGVEAARAEACLQIEFADGRLPVVPGETAKP
                     RRKPATPPADQGTLL"
     gene            complement(7215..8231)
                     /locus_tag="P5558_12285"
     CDS             complement(7215..8231)
                     /locus_tag="P5558_12285"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRY25134.1"
                     /translation="MGYRVAVAGATGAVGQEILNILEELRFPADEIIPLASERSVGKE
                     VAYGDDKRLKVRDLATFDFEGVDLGLFSPGAAVSAVQAPRAAAAGCVVIDNTSHFRMH
                     PEIPLIVPEVNPEALAEFRNKNIIANPNCSTIQMVMALKPLHDIARIRRVVVATYQSV
                     SGAGQEAMDELFDQTKSIYMNDKQEPKIFTKQIAFNCIPRIDSFLEDGSTKEEWKMTV
                     ETRKILDPSIEVSATCVRVPVFVGHALAVNVEFEEPLEPDEARHALHEFPGILVIDRP
                     EEDEYATQIDCVGDHATFVSRIRKDPTVPHGLNLWVVADNLRKGAALNTVQIAQALVE
                     EGYI"
     gene            complement(8253..9365)
                     /gene="leuB"
                     /locus_tag="P5558_12290"
     CDS             complement(8253..9365)
                     /gene="leuB"
                     /locus_tag="P5558_12290"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRY25135.1"
                     /translation="MNQVGNLLLLPGDGIGPEVMGEVQRVVGWFRDRQGLGFECETDL
                     VGGSAYDAHGTPLADSTLALAKAADAVVFGAVGGPAYDQLEFRLKPEQGLLKLRKELE
                     LFANLRPAMVFPALAEASSLKKELVEGLDILILRELTGGVYFGEPRGIEELPDGTRRA
                     VDTQVYTTAEIRRIASVGFELSRKRQGRVTSVEKANVMHTGVLWRQEVTKLHQEQFPD
                     VKLEHMYADNCAMQLVRRPKQFDVIVTDNLFGDLLSDAAAMLTGSLGMLPSASLGAPD
                     QAGRRKALYEPVHGSAPDIAGQGKANPLAMLLSFAMMLRYSFDLGAEADRVEKAIGAV
                     LDKGYRTADIMQPGMREISTTAMGQAVLDELDASLG"
     gene            complement(9444..10067)
                     /gene="leuD"
                     /locus_tag="P5558_12295"
     CDS             complement(9444..10067)
                     /gene="leuD"
                     /locus_tag="P5558_12295"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRY25136.1"
                     /translation="MQAFKTLTGIAAPLPMINVDTDMIIPKQFLKTIKRTGLGKNLFD
                     EMRYDQQGKEKPEFVLNRAAYRQAEVLIAGENFGCGSSREHAPWALLDFGIRCIIAPS
                     YADIFFNNCFKNGILPIILPQETVDLLMKEAENAQGRPFTIDLESQTITRPTGNEPIH
                     FEVDAFSKHCLLNGLDDIGLTMVKAKSIDGFEARQKAAEPWLHSSAA"
     gene            complement(10153..11235)
                     /gene="corA"
                     /locus_tag="P5558_12300"
     CDS             complement(10153..11235)
                     /gene="corA"
                     /locus_tag="P5558_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRY25137.1"
                     /translation="MKRRLFLKRRTKPGAAPGLLIVDPTAAATGITAIAYGPDRLEEI
                     PACPSARVANLRGSRAVLWVDIAGLADHAAISEVATAFGVHPLAVEDAVNTHQRPKAE
                     SYDGHVFVVFRMLTAESGAEGEQVAMAVGRDFVLTFQERPGDCFEPVRERLRHGKGRV
                     RQLGPDYLGYALIDAAVDSYFPYLETLGEEIEDLEDMVVKDPEPGHVERVHRVKRDLL
                     SLRRAVWPMREMLNALLRDEHEEISATTRTYLRDAYDHAIQLMDMVETYREIATSLLD
                     VYLSSQSARMNEIMKVLTIISTIFIPLGFIASLYGMNFDTSSPWNMPELAWRFGYPAV
                     LGLMAAIAFGLLWSFFRRGWLGGDRD"
     gene            complement(11293..12828)
                     /locus_tag="P5558_12305"
     CDS             complement(11293..12828)
                     /locus_tag="P5558_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRY25138.1"
                     /translation="MSLGSAPDALALDVPVASRITAVTVQLDRATVRREATVEIGRGD
                     HMLVFADLPAALDRRSLNLAGPGVVLGRPVFREIETLGPVNPAAARLAVELDTLERRR
                     RIERDRMTVQQLVLDVLRQPNMQPAIGGENQLAELDGLIGLVETRSSEALRRIRAAQD
                     DIDGLDADIARVEREIARLGEDPMRQLEVTVPVRVRSAGPIEIDIGYEVQGASFSPTY
                     EARLATGTTMLQLVALAEVSQHTGEDWTDVELTLSTAATSWQTAAPPAEPWYIDIWVE
                     QPRPAPMAARASAEKVSADFAAVAIDRSAFDATYRLPGRVSIAADGSAHRVTIEAVTR
                     AAELLWRALPEFDPTAYLTASLDHAGEVPLLPGPVFLYRDGASIGETHLAGLQPGEPL
                     ELGFGADPAVEIERRLLTDQRSATGLIGTTRQHERLFATTVTNRRTGPVTLELFDRLP
                     VPRDTRITVERLPETTPPALTAPDDRPGVLVWQLELPPGATETVTFGYRVRHPADLRV
                     TGF"
     gene            13066..15507
                     /locus_tag="P5558_12310"
     CDS             13066..15507
                     /locus_tag="P5558_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY25139.1"
                     /translation="MRTTARVVVIGGGVVGVSALYHLAKLGWTDCVLLERTELTAGST
                     WHAAGLLPLFNMSYSVGQLHQYSVELYKKLEAETGQDVGFHQTGNLRLATNRDRMDEY
                     RNYQCTAETIGVECHLIGVDEIKKLWPLVNAAGLVGALWHPTDGHIAPVDVTMALAKG
                     ARAMGAEIVQRCAVTGIERESGEWLVKTARGDIRCDHVISATGNYARTTARMVGLEIP
                     VVPVEHQYIVTDIDPALRDYRATGAKELPILRESDASYYLREERLGWILGPYERHAPA
                     CLVDGVPASFEKDLFPGDLERLMPHVEACMSRVPSFEQAGIKDIVNGPIAYTPDGNPM
                     VGPAFGLPNFWVSEGHSFGVTAAGGAGWQLANWIVEGEPSVDMIGVDPRRFGVVSKHF
                     ARLKNAEAYEHVFVIHYPMEERPACRPAKAPPVYDELKAAGAVFGQRFGWERPMWFAP
                     AGVAPRDVWSFRRSNWFEHVGREVEAMRERVGLMELGSFAKYTVEGPGARAFLDGMVA
                     NAIPKSIGRINLCHALNPSGSVRSEFTIARLPDGLWGERFYLVGPGAGHDYDWDFLTK
                     SIPRDGSVIVQDVTTQMGVFVLAGPHARKVLAKLADVDVSNEAFPWLTVRDIPVGFCP
                     SVRALRVNFVGSLGWELHHPIEYQRQLFEALLAAGRDEGIAHVGLRAMDSMRLEKSYR
                     LWGTDLNAENSVLEAGLDRFVRLQKGAFTGRDALIRQIEQGIPNRFCTIEIDADDADP
                     FGNEPVLMDGQVVGRGTAGGYGHHVKKSLMLGYVKSAFVEVGRACQVRVLGELRPARI
                     IAESPYDPENAALRA"
     gene            15704..>16232
                     /locus_tag="P5558_12315"
     CDS             15704..>16232
                     /locus_tag="P5558_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25140.1"
                     /translation="MTFFITSAGPGKGADLGGLAGADAQCQSLAEAAGAGGHTWRAYL
                     GSAEGDPRDRIGAGPWHNAEGVLIAESVEALHSDANAITKETGLTEQGEVVNGRGDTP
                     NRHDILTGANADGTTAADQTCGDWTLSGEGTAMVGHHDRMGLRDDAPSKSWNASHGSR
                     GCSQEALQGTGGDGLF"
BASE COUNT         2500 a         5530 c         5484 g         2718 t
ORIGIN      
        1 gacagcatga acagcccgaa catgatcctc gatctgcgca tcccgcagaa ccagaagtac
       61 cagcaggtcg tgctcgacga ggcgatctcg cgcttcctcg cgggtgagat cgacaaggag
      121 gcgaccgtcc aggcggtcgc gaatggctgg aacgagctga acgacgagat cggtgtggac
      181 gagcagctcg aaatctatcg ggccaccatc ggcgccaact gatcggtcgg cgacaaccgg
      241 gtccagtgcg ctcgtcgtgc cggacccgga gccgcccgtt ctccggtcga tgcacgagcc
      301 ctcatgacag ctttcgccga gacggccgtc gacaccgtcc cgcggccggg atggggcgag
      361 cggctcgacc ggcatgccgg ccgcctcatg gtcctgccgg cggtcgtcat cctgctctgc
      421 ttcgcgatct tcccgctgat catctccgcc tatctggccc tgtcgcgttt tgccctggcg
      481 cccggtggct tcacgctcaa gttcatcggg ctcggcaatt tccgcaaatt gctcaccggc
      541 tctcagcagt accatttcct cggcacgttc ggcgccttcg gcccggtcgc ctggggcttg
      601 gtggcgctgg cggccatcgc cctgctctat tgcatggcgc gctatctgag agggggcagc
      661 gtcacggtcg tcggcatcgt cgggcggctg atctccgtca gcctgacaat gggcctcgtc
      721 ctcgtcgccg tggcgacgct cgccacgagc ggcgtccccg gcacgctcgt caccaccctg
      781 ctctatgtcg tgatcggcgt ctccgtgcag ttcctcctcg gcctcgggct cgccctgctc
      841 tgcgcccagc cgatccgcgg ccgcaatttc tttcgcgtgc tctatttcgt gccgctgatg
      901 gtgactccgg tcggcatcgc ctacaccttc cggatgctgg cggacatgca gaaggggccg
      961 ttcgcgccgc tcgtccgctc gttcggcgtc ggtgaatggt cctgggcgac cgaggcctgg
     1021 tcggcccgcc tcatggtgct ggtcggcgac acctggcagt ggacgccgtt catgttcatc
     1081 gtgctgctag cggccatcga gaaccagccg cgcgaccagg tcgaggcggc gcggatcgac
     1141 ggcgccggcg gcctgcagat ctttcgcgac atcacctggc cggcgatagc cccggttgcc
     1201 gggacggtgg tcctgatccg cctgatcgag gccttcaaga tcatcgacct gcccaacgtg
     1261 ctgaccggcg gcggcccggg cctcgccacg gaatccatga cgctccacgc cttcatcgcc
     1321 tggcggacgc aggatctggg cgcttcggca gcggtcggct acatgctgct cttcgtctcg
     1381 accgtggtct gcatcacctt cttcaacttc gtggtccgac catcacgccg gctcgaatca
     1441 tgaacggcga ccgccgctcg ctccgccagc gcctgttcga gcccgagtcg ctcgagcgcc
     1501 aggcgccggt gccgaagttc atgacctacg ttctcctggt cttctggtcc ctggtcgtcc
     1561 tcgtgccgct ctactgggtg ctgatcacgt cgttcaagca gtccggggat gtcgacaacg
     1621 ggcctttcta catcccgttc gtcgacttca cgccgtcgct gcacgcgtgg gacttcatgc
     1681 tgctgcagaa caacacgctc ggaccctaca tcaactcgat cgtggcagcg ctcggcagca
     1741 cagtcgtcgg cgtggtagtc ggtgcgctcg cggcctatgc cctggtccgc acgcggctcg
     1801 aggtgaagct cgcgaccgtc gtgaccttcc tcctgctcct cgcggccacc gtcgccgcgg
     1861 tggtgactct gggcgtgccc tggccgatcg cgctcggctc ggcggtcgtc atcttcatcc
     1921 tcgcggtctt caccctgacg caccgggcga ggctggcgct cggcaatcgc gacatcgaat
     1981 tctggatcat ctccaatcgc atcatgccgc cgatcgtggc ggtgctgccg atctacatca
     2041 tgttccagca gctccggctg ctcgacacgc agctcgccct ggtcgcgacc tatgccgccg
     2101 tcaacctgcc gatcgtcgtc tggctgaccc gcgacttctt cgccgccatc ccggtcgatc
     2161 tcgaggagag tgcggagatc gacggcgcct cgaagttccg ggtgttcttc acgatcgccc
     2221 tgccgctggt ccgctccggc ctcgtcgcca ccttcctcct cgtcctcatc ctggcctgga
     2281 acgagtatct gctggcgctt ttcctcacca acgcgaacgc gcagaccatg ccggtcctgg
     2341 tctcggcgca gaacaccacc cgcgggccgc aatggtggaa catgtcggtc ctgatcgtcg
     2401 tcatgatcgc ccctgtcgtg gtcatcgcga ccgtcctgca gaagcacatc gcgcgcggcc
     2461 tcctggtcgg cgccgtcaag ggctaggatc aggatgcgcg tcaccctctc caacatcttc
     2521 aagtcgttcg gcgcggtcca ggtggtcaag ggcctcgacc tcgagatcga agacggcgag
     2581 ttcctcgtgc tgctcgggcc gtcgggctgc ggcaagacaa cagcgctgcg gatggtcgcg
     2641 ggcctcgaat cggtcacgtc ggggcggatc gccatcggcg agcgggacgt cacccaggtg
     2701 ctgccgaaat atcgcgatgt ggcgatggtc ttccagtcct acgcgctcta cccgcacatg
     2761 acggtggcga agaacatcgg ctatcccctg aagctgcgcg gcgtgccgaa ggcggagcag
     2821 gagacccaga tccgggccgc cgcggagaag gtccatctcg aggactacct ggatcgcttt
     2881 ccccggcagc tctcgggcgg ccagcggcag cgcgtggcgc tggcccgcgc catcgtccgg
     2941 cggccgagcg tctttctcat ggacgagccg ctctccaacc tcgatgccaa gctgcgcagc
     3001 tacatgcgcg ccgagctgaa gcatttgcag gaggagctcg gcatcaccac catctacgtc
     3061 acccacgacc agatcgaggc gatgaccctc gcccaccgcg tcgcggtgat gaaccagggc
     3121 gtggtccagc agatcgcgac cccgcgcgag atctacgaca acccggcaaa tctcttcgtt
     3181 gccggcttca tcggctcgcc gccgatgaac acgctgaccg ggccgatcga ggacggcagc
     3241 ttcgtcctgc cggagggcag ggtggcggtc ggcaactatg ctccacggtc cgaagccgtc
     3301 ttcggctttc gtcccgagga cgcgcagatc gtgtcgccgg atgccgccga cctgcaggcc
     3361 agggtcttcg cctgcgagct caccggcaac gagaccatcg tcacctgcga ggtcggcaag
     3421 gcaccggtcg tggtcaagat ggacaagaac tacgacgtcc gcgccggcac cgctgtcggc
     3481 atcgccatcg accgtgccaa gctttgcctc ttcgatgccg ggacaggcga gaagatcgcg
     3541 gccggcggat agggccctgc cgcgaccccg tcctcagatc gagccggctt cgaccatgta
     3601 gttgttggcg gtgcacatcg ccgcatcgtc ggaggcgagg aagagcacca tccgggccag
     3661 atagaccggc tggatcaggt ccggcaggca ttgccgctgg cggtgcttct cgagcgcctc
     3721 cggtgtcgcc cagagcgtct tctgccgttc ggtcatgatc cagcccggca ccaccgtgtt
     3781 gacccggatc cggtgctggc cgaggtcgcg ggccatgccg cgggtcaggc cgtgcacggc
     3841 cgccttggcc tgggtgtaga cgggaaaccc gccgcccgcc tcccaccacg agttcgagcc
     3901 gaagttgatg atcgagccct tgccggcctt gatcatgccg ggtgcaaccg cctggatggc
     3961 gaagaacatg tggcggagat tggtggcgat gcgctcgtcg aagtactccg gcgtcacgtc
     4021 ctgccagcca tgccggtcgt cgcgcgcggc gttgttgacc agcaccgtgg taggaccctg
     4081 ggccgcctcg accgcggcga atgcccgttt cagggcctcg atgtcacgca ggtcacaggt
     4141 ctcgaaatgt accttggcac cggcatcgcg caactcgccc gccagcgccg tgccgcggtc
     4201 ggtgtccagg tcgacgaagc ccacccggct gccctgctcg gcaaaggcgc ggacgatctg
     4261 ctcaccgatg ccggaagcac caccggtgac caggacggcg acgtcatcga ggctcggata
     4321 gcgggcaaac gacattgggc gatcctccgg attctgggtc ggttctgtcc tctgtgagca
     4381 tcgccccaag gcgtcaagcc cccgatgcct tgcgatccgt aactgcctca gttcacacct
     4441 ttcggctcat cgaccggcgc cgccccgacg agaccgccct cggtgcgcgc gccctcccag
     4501 tcgggcaggg cttcgatctc cttggcggtg aagtcgacga tcaggcgccg cggcgcctcc
     4561 tgcatctcga cccggtgcca gggaacggcc acgcgctcgg ccccgacgcc gatatcaatg
     4621 ccgagaatct cgccgccacg ttggatgacc aggaacttca ggaggccgtc ctccgggtcg
     4681 gcgacgaggt cgtagacgtc gccgagctcc tcgtcgtcgg cagacaccac gtcgagctcg
     4741 agcaagcttg tgaccgagac ggcaccggtg gtggggggca gctccgccgc tcccggcgcc
     4801 acatcgacct ggggtgtctc ggcaagcgca tcggtggcgg cttcctcggc cacggcgggc
     4861 accgccccca agagcgccat gaacgacagg gcgagcccgg ccttcgtcga tcggttggtc
     4921 ggcatcgctg ctcccttcgg attcctggat gatcggttgt cgaaccgatc gacccgctcg
     4981 gcggttccgt ccaaccggcc tcagcccgtc ttgacgcgct gccagagggc ctcgagggcc
     5041 tcgaggtcga gctcgccgag cggcctgtcg ctcgtccgct ccatccgccg aaaacgcgat
     5101 tcgaacttgg cgttggcccg cctgagcgcc gtctccggat cgagcccgag ccggcgcgcg
     5161 tggttgacga gggtgaagag gaggtcaccc agctccagct cgcgctcgtc cggggcggcg
     5221 ctttccagct cgtccagctc ctcgttgacc ttgtctcgca caccttggat gtcgggccag
     5281 tcgaagccta cgcgaagcac tctcttctgc agcttgagcg cccgggtgag agccggcagg
     5341 ccgtgcggca ggttgtcgag gaggctggcg gcactcccgt cggatgcggc cttggcttgg
     5401 cgctcggcgg ccttcgccga ttcccagtgc tcgtgctggg cttccgcacc tttgacctcc
     5461 acgtcgccga agacgtgcgg gtggcgagcg atcatcttgt cggcgatcgc cgtggccacg
     5521 tcgtcgaagc cgaaaagccc ggcctcggcg gccatctgcg cgtgatagac cacctgcagg
     5581 aggagatcgc ccagctcgcc tcgaagctcc tcccagtcgg cccgctcgat ggcatccgcc
     5641 acctcgtagg cctcctcgat cgtatagggg gcgatggtgg cgaaggtctg ctcgatgtcc
     5701 caggagcagc caatctcggg gtcgcgcagg cggcgcatca cggcgagcag gcgatcgatc
     5761 ggccgctcgg cgctcaaagc agcgtgccct ggtcggcagg cggcgtggca ggcttgcgtc
     5821 gcggcttcgc cgtctcgccg ggcacgaccg gcaggcgccc gtcggcgaac tcgatctgga
     5881 gacaagcctc ggcgcgcgcc gcctcgacgc cggtgaccag gccgcccgaa gcgccgtgga
     5941 cgagcgcgta tccccgatcc agcacgcggc gatggctgag gctctccagc agcttggcgg
     6001 gaccgtcgag gctgcgctgc agctcgcgca ggcgatcagc gatgcgctgg tcgagcgtct
     6061 cggccggcag ccgcgccaag tcacgctcgg cctgccggac gcgatccagc accagctcgc
     6121 cgagccgggc atggcgcccc ccgagcttct cggcctgatc gtcgagcacc gccagtggtg
     6181 ttcgcaggcg cagccgctcg gtgaggtcgt cgagccgctg ctcggcgaag cgcaggatgg
     6241 ccaagggatc ggggaggcca cgggcgagtc cgtcgagtcc ctggcggcgg tcccgcaggc
     6301 cacgttcgag tgcatggatc agccgctcgg ccagtccctc ggtcgaggcc agaagctctg
     6361 cccggaccgg caccgccatc tcggccgcgg cggtcggcgt gggcgcccgg cggtcggcga
     6421 cgaagtcgat gagcgtcgtg tcggtctcat ggccaacggc cgagatcaat ggaatcgagc
     6481 aggcggcgac ggcgcgcgcc accgcctcct cgttgaaggc ccagagatcc tcgatcgatc
     6541 cgccgccccg ggcaacgatc agcaggtcgg gtggggggat cgctccctcg gcggcgagac
     6601 ggtcgaagcc tccgatggcg gccgcgatct gctccgccgc accctcgccc tggaccgcca
     6661 ccggccaaag caggacggac agggggaatc gttcggccag acggtgcagg atgtcgcgca
     6721 gcacggcacc ggtgggcgag gtgacgagcc cgatgcagcg gggcagatag ggcaggggcc
     6781 gcttgcgctc cggctcgaac agaccctccg cagcgagacg ttgcttgcgc tcctcgaaga
     6841 gcgccatcag ggcgcccacg cctgccggct ccatgtgctc gatgacgagc tggtagcgcg
     6901 agcgaccggg ataggtggtc agccggccgg tggcgatgac ctccagccca tcctccggct
     6961 tgaagccgag cgcccccgcc gtgccgcgcc agcagacgcc atcgagaacg gccttgtcgt
     7021 ccttcagcgc caggtagaga tgcccgctcg aggcgcgctt gaagccggag atctcgcccc
     7081 tgacccggac caggccatag gcatcctcga tcgtcttctt cagggcgaag gcgatctcgg
     7141 agaccgagta ctcgtgcagg ttggagacca tggcctcggg cttaccagcc gggcgaccgg
     7201 ccgtcgaccg caggctagat gtagccttcc tcgaccagcg cctgggcgat ctggacggtg
     7261 ttgagcgctg cacccttgcg caggttgtcg gccaccaccc acaggttcag gccgtgcggc
     7321 acggtcgggt ccttgcggat gcgcgagacg aaggtcgcgt ggtcaccgac gcagtcgatc
     7381 tgggttgcgt actcgtcctc ctccggccgg tcgatcacca gaatgcctgg gaactcgtgc
     7441 agcgcgtggc gtgcctcgtc cggctcgagc ggttcctcga actcgacatt gaccgcgagc
     7501 gcatggccga cgaagaccgg aacacgcaca caggtggcgc tcacctcgat cgacggatcg
     7561 aggatcttgc gggtttcgac cgtcatcttc cactcctcct tggtcgagcc atcttcgagg
     7621 aagctgtcga tccggggaat gcaattgaag gcgatctgct tggtgaagat cttcggctct
     7681 tgcttgtcgt tcatgtagat cgacttggtc tggtcgaaga gctcgtccat cgcctcctga
     7741 ccggcgccgc tcaccgactg gtaggtggcg acgacgacgc gcctgatccg ggcgatgtcg
     7801 tggagcggct tgagcgccat caccatctgg atggtcgagc agttggggtt ggcgatgatg
     7861 ttcttgttgc ggaactcggc cagcgcctcc ggattgacct ccggcacgat cagcgggatc
     7921 tcggggtgca tgcggaagtg cgaggtgttg tcgatcacca cgcagcccgc cgccgcggcg
     7981 cgcggcgcct gcaccgcgga gaccgccgca ccgggcgaga agagaccgag atcgacgccc
     8041 tcgaagtcga aggtggccag gtccctgacc ttcagccgct tgtcgtcgcc ataggcgacc
     8101 tccttgccga ccgagcgctc ggaggcgagc ggaatgatct cgtccgccgg gaagcggagt
     8161 tcctcgagga tgttcaggat ttcctggccg acagccccgg tggcgccggc tacggcaacg
     8221 cgatagccca tgatttcaac cttttgcggt gctcagccga gagaagcgtc gagctcgtcg
     8281 aggacggcct ggcccatggc cgtcgtcgag atctcgcgca tccccggctg catgatgtcg
     8341 gcggtccgat agcctttgtc gagcacggca cctatcgcct tctcgacgcg gtcggcctcg
     8401 gcgccgaggt cgaaggagta gcgcagcatc atggcgaagc tgagcagcat ggcgagcggg
     8461 ttggccttgc cttggccggc gatgtcgggg gcggagccgt gcaccggctc gtagagcgcc
     8521 ttgcgccgac cagcctggtc cggagcaccg agcgaggccg agggcagcat gccgagcgag
     8581 cccgtgagca ttgccgccgc gtccgagagc aggtcgccga agaggttgtc cgtgacgatc
     8641 acgtcgaact gcttgggccg ccggacgagc tgcatagcgc agttgtcggc atacatgtgc
     8701 tcgagcttca cgtccgggaa ctgctcctgg tggagcttcg tcacttcctg ccgccagagc
     8761 acgcccgtgt gcatcacatt ggccttctcg accgaggtca cgcgaccctg gcgcttgcgg
     8821 ctgagctcga agccgacgct ggcgatgcgg cggatttcgg ctgtggtata gacctgagtg
     8881 tccaccgccc gccgcgttcc gtcaggcagc tcctcgatgc cgcgcggctc gccgaaatag
     8941 acgccgccgg tcagctcccg caggatgagg atgtcgagcc cctcgaccag ctccttcttg
     9001 agagagctcg cctcagccag ggccggaaag accatggccg ggcggaggtt ggcaaagagc
     9061 tcgagctctt tcctcagctt gagcaggccc tgttccggct tcaggcggaa ctcgagctgg
     9121 tcgtaggccg gaccacccac ggcaccgaac accacagcgt cggcagcctt ggcgagggcc
     9181 agcgtgctgt cggccaaagg cgtgccgtgc gcgtcatagg ccgagccgcc gacaagatcc
     9241 gtctcgcact cgaagccgag gccctggcga tcgcggaacc agccgaccac gcgttgcacc
     9301 tcgcccatca cctcggggcc aatgccgtca ccgggcagaa ggagcagatt tccgacctga
     9361 ttcattgtcc tgcaatcgaa tgatgatgcg ttcctgagcg gcgtcgccgc cgcggtggtg
     9421 ctgcggcgaa tgagcgagaa ggctcaggcg gcgctgctgt gaagccaggg ctcggcggct
     9481 ttctgcctgg cctcgaagcc gtcgatcgac ttcgccttga ccatggtcag cccgatatcg
     9541 tcgaggccgt tcaacaggca atgcttgctg aatgcatcga cctcgaaatg gatcggctcg
     9601 ttgccggtcg gccgggtgat cgtctggctc tcgagatcga tggtgaacgg ccggccctgc
     9661 gcgttctcag cctccttcat caggaggtcc accgtctcct gcggcaggat gatcgggagg
     9721 atgccgttct tgaagcagtt gttgaagaag atgtcggcat aggagggggc gatgatgcag
     9781 cgaatgccga aatcgagcag cgcccatggc gcatgctcgc gcgacgagcc gcagccgaaa
     9841 ttctcgcccg cgatcagcac ctcggcctgg cgataggctg cccgattgag cacgaactcg
     9901 ggcttctcct tgccctgctg gtcgtagcgc atctcgtcga agaggttctt gccgagcccg
     9961 gtccgcttga tcgtcttcag gaattgcttg ggaatgatca tgtcggtgtc gacattgatc
    10021 atcggcaggg gggcggcgat cccggtaagg gtcttgaagg cttgcatgag cggcttccgc
    10081 tgggcgtcga gatgccgcgt gtcataggcg agcggttcgc ctgcggcaag cgggtgacgg
    10141 cagaggcgcc tcttagtccc ggtcgccgcc gagccagcca cgtcgaaaga acgaccagag
    10201 gaggccgaag gcgatcgccg ccatgaggcc gaggaccgcc ggatagccga agcgccaggc
    10261 gagctcgggc atgttccagg gcgagctcgt gtcgaagttc atgccgtaga ggctggcgat
    10321 gaagccgagc gggatgaaga tcgtcgagat aatggtcagg accttcatga tctcgttcat
    10381 tcgggcactc tggctcgaca gatagacgtc gagcaggctg gtcgcgattt cgcgataggt
    10441 ctcgaccatg tccatgagct ggatggcgtg atcgtaggca tcgcgaaggt aggtgcgggt
    10501 ggtggcgctg atctcctcgt gctcgtcgcg cagcagcgcg ttcagcatct cgcgcatcgg
    10561 ccagaccgcg cggcgcaggc tcagcaggtc acgcttcacc cggtgcacgc gctcgacatg
    10621 gccgggctcg ggatccttga ccaccatgtc ctcgagatcc tcgatctcct cgccgagcgt
    10681 ctccagatat gggaaatagc tgtcgaccgc ggcatcgatc agcgcgtagc cgagatagtc
    10741 ggggccgagc tggcggaccc gccccttgcc atggcgcagc cgttcgcgca ccggctcgaa
    10801 gcagtcgccc ggccgctcct ggaaggtcaa gacgaagtcc cggccgaccg ccatcgccac
    10861 ctgctcgcct tccgccccgc tctctgcagt cagcatgcga aagacgacga acacatggcc
    10921 gtcgtagctc tccgccttgg gccgctgatg ggtgttgacc gcatcctcga cggccagcgg
    10981 atgcacgcca aaggccgtgg cgacctcgct gatcgccgca tgatcggcaa gtcccgcgat
    11041 atcgacccag agcacggccc tcgagccgcg cagattggcg acccgcgccg acgggcaggc
    11101 ggggatctcc tccagccgat cgggaccata ggcgatggcg gtgatgccgg tcgcggccgc
    11161 cgtcgggtcg acgatgagca gaccgggtgc cgcccccggc ttcgtgcgtc gcttgaggaa
    11221 caggcggcgc ttcatgcggc gcaatggtcg gctccctcca cggcaatcgg tccctgacgc
    11281 atcggccggc cgtcagaagc cggtgacccg gagatccgcc gggtggcgaa cgcggtagcc
    11341 gaacgtcacg gtctcggtcg ccccgggcgg cagctcgagc tgccagacca gcacgcccgg
    11401 ccggtcgtcc ggtgctgtga gcgcgggcgg tgtcgtctcg ggcagccgct ccacggtgat
    11461 cctggtgtcg cgcggcaccg gcaggcggtc gaagagctcg agcgtgaccg ggcccgtccg
    11521 gcggttggtg acggtcgtgg cgaacaggcg ctcgtgctgg cgcgtggtgc cgatgagccc
    11581 ggtggccgag cgttggtcgg tcaggagccg gcgctcgatc tccacggccg ggtcggcacc
    11641 gaagccgagc tcgagcggct cgcccggctg cagcccggcg agatgcgtct cgccgatcga
    11701 cgccccgtcc cgatagagga agaccggccc gggcagcagc ggcacctcgc ccgcatgatc
    11761 gagcgaggcg gtgagatagg cggtcgggtc gaactcgggc agcgccctcc agaggagctc
    11821 ggcggcacgc gttacggcct cgatcgtcac acggtgcgcg gagccgtccg cagcaatgct
    11881 gaccctgccg ggaagtcggt aggtcgcatc gaaggcgctc cggtcgatcg ccacggcggc
    11941 gaagtcggcc gagaccttct cggcgctggc ccgggcggcc atcggcgccg ggcgcggctg
    12001 ctcgacccag atgtcgatat accagggctc cgccgggggt gcggccgtct gccagcttgt
    12061 cgccgcggtc gacagcgtca gctcgacgtc ggtccaatcc tcgcccgtgt gctggctgac
    12121 ctcggcgagc gccacgagct gcagcattgt cgttccggta gcgagtcggg cctcataggt
    12181 cggtgagaac gaggcaccct ggacctcgta accgatatcg atctcgatcg gtcctgccga
    12241 cctcacccgc accggcaccg tcacctcgag ctggcgcatc gggtcctcgc cgaggcgggc
    12301 gatctcgcgc tcgacccggg cgatgtcggc atcgagaccg tcgatgtcgt cctgcgccgc
    12361 ccgtatccgg cggagcgcct cgctgctccg cgtctcgacg aggccgatca gcccgtcgag
    12421 ctccgcaagc tgattctcgc cgccgatggc cggctgcatg ttcggctggc ggaggacgtc
    12481 gagcacgagc tgctggacgg tcattcgatc acgctcgatc cgccgccgcc gctccagggt
    12541 gtcgagctcg acggcgagcc gcgctgccgc cgggttcacg gggccaagcg tctcgatctc
    12601 gcggaagacc ggccggccca agacgacgcc cggcccggcg aggttcaggc tgcgccggtc
    12661 gagcgcagcc ggcaggtcgg caaacaccag catgtgatcg ccccggccga tctcgaccgt
    12721 tgcctcgcgg cgcaccgtgg cccgatcgag ctgcacggtg accgcggtga tccggctagc
    12781 gaccggcacg tccagggcaa gtgcgtcggg tgcggacccc agggacacgc cgatcagcaa
    12841 ggcaatgacg cggcagctgc ccgggggcgc ggacggcagg tgacggcggt gcatcggcga
    12901 cgactcccag atgacagcgt gccagccact ttatgaccgt cccccctgcc gctgccaagc
    12961 cggcgggcat ttgtcgcttt ggcgagcctc gttgtcgcgc gcgcgcgtcc actgcccggc
    13021 atcgactgct agctcctacg ggcggcaagc gggggaagcg tggcgatgcg gacgacggcg
    13081 agggtggtgg tgatcggcgg cggcgtggtg ggcgtgagcg ctctctacca tctggcgaag
    13141 ctgggctgga ccgactgcgt gctgctggag cgcaccgagc tcaccgctgg ctcgacctgg
    13201 cacgcggctg gcctcctgcc gctcttcaac atgtcctact cggtcggcca gctgcaccag
    13261 tattcggtgg agctctacaa gaagctcgag gcggagaccg gccaggatgt cggcttccac
    13321 cagaccggca acctgcgcct cgccaccaac cgcgaccgta tggacgaata ccgcaactac
    13381 cagtgcacgg cggagaccat cggcgtcgag tgccacctga tcggcgtcga cgagatcaag
    13441 aagctctggc cactggtgaa tgcggccggg ctggtcggcg ccctctggca cccgaccgat
    13501 ggccatattg ccccggtcga cgtcaccatg gcgctggcca agggtgcccg ggcgatgggg
    13561 gcggaaatcg tccagcgctg tgcggtcacc ggcatcgagc gcgagagcgg cgagtggctc
    13621 gtcaagaccg cgcggggcga cattcgctgc gatcacgtga tctcggccac cggcaactac
    13681 gcccgcacca ccgcccgcat ggtcggcctc gagattccgg tcgtcccggt cgagcatcag
    13741 tacatcgtca ccgatatcga cccggccttg cgggattacc gggcgacggg ggcgaaggag
    13801 ctgccgatcc tgcgcgagag cgacgcctcc tattatctgc gcgaggagcg cctgggctgg
    13861 attctcggcc cctatgagcg acatgcgccg gcctgcctcg tcgatggcgt gcccgccagc
    13921 ttcgagaagg atctcttccc gggcgatctc gagcgactga tgccgcatgt cgaggcctgc
    13981 atgagccgcg tgccgtcctt cgagcaggcc ggcatcaagg acatcgtcaa cgggccgatc
    14041 gcctatacgc ccgatggcaa cccgatggtc ggcccggcct tcggtctgcc gaacttctgg
    14101 gtctcggagg gccacagctt cggggtcacc gccgccggtg gggcgggttg gcagctcgcc
    14161 aactggatcg tcgagggcga gccttcggtc gacatgatcg gcgtggaccc caggcgcttc
    14221 ggcgtggtct ccaagcattt cgcccggttg aagaacgcgg aggcctacga gcacgtcttc
    14281 gtcatccact acccgatgga ggagcggccg gcctgccggc cggccaaggc gccgccggtc
    14341 tatgacgagc tgaaggctgc cggtgccgtg ttcggtcagc gcttcggctg ggagcggccg
    14401 atgtggttcg ccccggccgg cgtcgcaccc cgtgacgtct ggtcctttcg ccgctccaac
    14461 tggttcgagc atgtcggccg cgaggtcgag gcgatgcgcg agcgggtcgg cctcatggag
    14521 ctcggctcct tcgccaagta cacggtcgaa ggccccggtg cgcgggcctt tctcgatggc
    14581 atggtggcca acgccattcc caagagcatc ggccgcatca acctctgcca cgcgctcaac
    14641 ccctcgggct cggtccgctc ggaattcacc atcgccaggc tgccggacgg gctctggggc
    14701 gagcgcttct accttgtcgg ccccggggcc gggcacgact acgactggga tttcctcacg
    14761 aagtcgatcc cgcgggacgg ctcggtcatc gtccaggacg tgacgaccca gatgggcgtc
    14821 ttcgtcctcg ccggccccca tgcgcgcaag gtcctggcga agctcgccga tgtcgatgtc
    14881 tcgaacgagg ccttcccctg gctcaccgtg cgcgacatcc cggtcggctt ctgcccgagc
    14941 gtgcgggccc tgcgcgtcaa cttcgtgggc tcgctggggt gggagctgca tcacccgatc
    15001 gagtaccagc gccagctttt cgaagcactc ctggcggcgg gcagggacga gggcatcgcc
    15061 catgtgggcc tgagggccat ggactcgatg cggctggaga agagctatcg cctctggggg
    15121 accgacctga acgccgagaa ctcggtcctg gaggccggtc tcgaccgctt cgtccgcctg
    15181 cagaagggtg cgttcaccgg cagggacgcg ctcatccgcc agatcgagca gggcattccg
    15241 aaccggttct gcacgatcga gatcgacgcc gacgatgccg atcccttcgg caacgagccg
    15301 gtcctcatgg acggccaggt ggtcggccgc ggcaccgccg gcggctacgg gcaccatgtg
    15361 aagaagtcgc tgatgctcgg ctatgtgaag agcgcgttcg tcgaggtggg ccgcgcgtgc
    15421 caggtccgcg tcctgggcga gctccgcccg gcccggatca tcgccgaaag cccgtacgac
    15481 ccggagaacg cggcgctcag ggcctgagcg agcccgctca cgcgattgcg ttcagccacc
    15541 cgatggccct gtgccgggcc ttgtggttcc atgcgcgcgt cggaccgttg cgaaaaccag
    15601 atggcaggga ggcccgtcca tgagagcatc gaccatagcc gcggcgtgcg cagcacttgc
    15661 gcttgcctgc accgccccgg tcgcgagcgc ccaggacgcc gagatgacat tcttcatcac
    15721 cagtgcgggc cccgggaagg gtgccgatct cggcgggctc gcaggcgccg acgcgcagtg
    15781 ccagagcctg gccgaggcag ccggcgccgg cgggcatacg tggcgcgcct atctcggctc
    15841 ggccgagggc gacccgcgcg accgcatcgg cgcggggccc tggcacaatg ccgagggtgt
    15901 gctcatcgcc gagagcgtcg aggccctgca cagcgacgcc aatgccatca ccaaggagac
    15961 ggggctgacc gagcagggag aggtcgtcaa cgggcggggc gacacgccca accggcacga
    16021 catcctgacc ggggcgaacg ccgacggcac gacggccgcg gaccagacgt gcggcgactg
    16081 gaccctgagc ggcgagggca ccgccatggt cggccatcac gaccggatgg gcctcaggga
    16141 cgacgcgccc tcgaagtcct ggaacgcctc gcatggcagc cgcggctgca gccaggaggc
    16201 gctgcagggc accggcggcg acgggctctt ct
//
LOCUS       DAOTFI010000108        16221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4326_length_16221_cov_6.375046, whole genome shotgun sequence.
ACCESSION   DAOTFI010000108 DAOTFI010000000
VERSION     DAOTFI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 16221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16221
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4326_length_16221_cov_6.375046"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="P5558_12320"
     CDS             complement(<1..914)
                     /locus_tag="P5558_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY25141.1"
                     /translation="MSRTSDLWWKNAVFYCLDVETFQDSDGDGIGDFAGLTSRLDYLA
                     GLGVTCLWLMPFYPTPNRDDGYDITDYYAVDSRLGTLGDFVELMRTARDRGIRVIADL
                     VVNHTSREHPWFRSARAGRESPYHDWYVWRDAIPEGGPEGLVFPDAEQSNWEWDEQAG
                     RFFLHRFYEHQPDLNIANPAVRAEIRKIVGFWLELGLSGFRVDAVPFLLETEGIATEM
                     KITPHDWLRDLSGFMARRAGGAIMLGEVNLEYDDVRSFFGDESGDELHMSLNFNLNQA
                     MALTLVRGDSGALIHNLRAMPALPEKSA"
     gene            1214..2131
                     /locus_tag="P5558_12325"
     CDS             1214..2131
                     /locus_tag="P5558_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25142.1"
                     /translation="MTLATESAANRPPATTSTVFAAEPRGSYVDWGAVIAGAVVAAAL
                     SLVLLTFGSALGLGLTSLEPGEGVSPLWLAIAAAIWLLWVQVSSFMAGGYLAGRLRRP
                     IGDAEPDEVDLRDGSHGLLVWGTGTLVGGLMLTSGLLGAVQTTAQATSNVAGAAVQAA
                     GSAADEGASFAGYLTDTLFRDAATGNSAQARSQARSEAGRIVSQALIDGEIPADDRAR
                     LVEIVAEQTGTEPAQVEARVATLEQRLQQQAEDAVEAAETARRWGVIAAFIAAATMLV
                     SAAGAYWAATMGGSHRDSGLLTPFWRRTR"
     gene            complement(2308..2799)
                     /locus_tag="P5558_12330"
     CDS             complement(2308..2799)
                     /locus_tag="P5558_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY25143.1"
                     /translation="MARKSLQPFAGFGGLAGGGEADPFGLMRREMDRLFDSYSRDWPA
                     PALAKGLMSPKVDVAETERGLEISAELPGIEQKDISLDLTDGILTLKAEHRAEKEEKD
                     EKKHYHLVERSRGSFMRRFAVPFEPDADKVQASFENGVLKIEVPRSAAAEKQVRKIAI
                     KGG"
     gene            complement(2939..3937)
                     /gene="glpQ"
                     /locus_tag="P5558_12335"
     CDS             complement(2939..3937)
                     /gene="glpQ"
                     /locus_tag="P5558_12335"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRY25144.1"
                     /translation="MRASGLGVAGALLAMLLGATLARAEPLVIAHRGASGYLPEHTTE
                     AQALAVGLGADFVELDLVLSRDGVPVVLHDLTLDATTDVAEHFPGRARADGRYYAIDF
                     DRAELATLGVGERVDPKTGEAVFPGRFPLTSGGFGLPTLARTIDLVQGLEASTGRSIG
                     LYVEIKRPGFHRDEGQDISRVVLEALAAAGYGGQGDGRLYLQGFDWAETRRIRDELGY
                     KGPLVQLIGGNDWADAAGSDYEALLGDEGLLELAGVVDGIGVPIGLVLDRAGESPGLA
                     ERVTARGLAVHAYTLRADRLPGWAADMAELVASLEAVGVDGFFTDQPDLTRAALGR"
     gene            4024..4593
                     /locus_tag="P5558_12340"
     CDS             4024..4593
                     /locus_tag="P5558_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25145.1"
                     /translation="MARKTDDIEGEVVTAALDIAEAEGWPAVRLHRIADRTGLPLERI
                     GARFRDVDAIADCWFDRARAALLATPEADLAGRTGPERLAVAIERWLDALEPHRRATL
                     QMLKGKLYPSHPQHWVPLVFSLSRLVHDILDVARLAGDGRRRQAQEVTLTLVLLATLA
                     QWARDRSEGQARSRAGLRRRLRRSVLQDA"
     gene            4590..5228
                     /locus_tag="P5558_12345"
     CDS             4590..5228
                     /locus_tag="P5558_12345"
                     /inference="COORDINATES: protein motif:HMM:NF021141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HRY25146.1"
                     /translation="MSIRAASLALVWLAACGPALPEPPPATAALGAATTIQVTANGWH
                     TGIVVPVAALTPGVLPERADFPAARHLELGWGDAAYYPMPDPPPGAVLRAAFAGGPAV
                     LHVTGHVYRPEPGQGRTVVEIDLTAAGLAALLRALDASFQRPASPRPGLDPASRFYPA
                     TGRFSLFNTCNSWTARQLATAGVAVDPSGVITAGQLLSQLDDLPQPLVMPAS"
     gene            5225..7660
                     /gene="hrpB"
                     /locus_tag="P5558_12350"
     CDS             5225..7660
                     /gene="hrpB"
                     /locus_tag="P5558_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HRY25147.1"
                     /translation="MSEAPQLREAARSLPVAEALPALRRTLADAGMAVLTAPPGSGKT
                     TLVPLLLQAEPWLAGGSILMLEPRRLATRQAAERMAELLGEKVGETVGYAMRFERRTS
                     SATRLEVVTEGLFVRRLQADPGLDGVGLVIFDEFHERSLDADLGLALAREVRSSLRPD
                     LRLLVMSATLDAEAVSGFLDDAPIIRATGRQHPVALHHLPGTGPAEAARTALDRQPGD
                     VLVFLPGAREIERIAGELAGTPAEIHKLHGALPRARQDAALRPSPGRRKVVLATNIAE
                     TSLTIEGIGSVVDAGLARRSRYSPRTGMSRLETVRVSRASADQRSGRAGRLGPGHAWR
                     LWPEAETLGLAPADPPEIAEADLAPLVLELAIWGVRTPDGLAMPTAPPESAWRAARDL
                     LMALDALDEGGDPTPHGRAMAALPVHPRLAHMLLEAARHEAVGTALALAALLGERDPW
                     SGRLGPDLADRLARWPEAPPTLRRTARQLARRLGVAETPPDPAMAGLCAALAFPDRIA
                     QRRRKGGAELRLVNGRGARLVAAPELDPFDFLVVAESEDAGSDARVRLAAGLAKDDLD
                     RLAGHRVTASRTVAFDAERGAIQAEGQARLGALVLARRPVAVSDDEALPVLLDAIRRQ
                     GLAILPWSGSTEALRQRLAWLARIEPDAWPPVDEATLLAELGLWLAPWLAGIRRLAEL
                     DPASLARALLARLDPASRSRLDRLAPTHWTTPLGSTLAIDYNSDPPAIACRLQELLGL
                     DSHPTVAASRPLRLALLSPAGRPIQVTDDLPGFWRGSYALVRKDLRGRYPKHPWPEDP
                     LRATPWRPGLARG"
     gene            complement(7671..8717)
                     /locus_tag="P5558_12355"
     CDS             complement(7671..8717)
                     /locus_tag="P5558_12355"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY25148.1"
                     /translation="MHWGGVVPWENARKAIYADNGETSRMVPENGRHVVMPGETVSGL
                     AELYHVGMVDLVALNGLSAPYTIYVGQVLRLPRPGETAIAARNPARGAPVQAASAPPS
                     GGPTHTVVRGDSLARIAAANRVGLGDVIALNPGIDPDRLMVGTRVRLPGGSGPQTAVA
                     ARAAPASDRTVAAAPAARPQVVPVATRPAAPAAAEQAARQRDLAAVAAPALSGAGFIW
                     PLESGTVISRFGSKPDGRQNDGINIAAPAGSLVRATENGLVVYAGEDLAAFGRMLLVR
                     HADGYLSAYAHNDTLLVARGDVVQRGQPLAKVGTSGEVVEPQLHFEIRRGKAPIDPLK
                     MLRDGSVEIAQRSE"
     gene            complement(8976..9764)
                     /gene="surE"
                     /locus_tag="P5558_12360"
     CDS             complement(8976..9764)
                     /gene="surE"
                     /locus_tag="P5558_12360"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRY25149.1"
                     /translation="MLKPPGKKPRILVTNDDGINAPGLKVMEAIAREISDEVWVVAPE
                     INHSGAGHSLTLRRPLRTRRLSERHYAVDGTPTDCVLLALQNILEDKAINLVLSGVNH
                     GHNLGEDVTYSGTIAACMEATLLQVPAIAFSQACHERHPVKWKTAEQWAPGVIRPMLS
                     MEWPTDVLVNLNFPDVVAGSVKGVKVTRQGKSKIGDELIERIDPRGEPYVWIGLSRDE
                     AEHADDTDVFAVRAGWVSVTPIQLDMTHRESFAKLTKLLQSQQE"
     gene            complement(9745..11034)
                     /gene="serS"
                     /locus_tag="P5558_12365"
     CDS             complement(9745..11034)
                     /gene="serS"
                     /locus_tag="P5558_12365"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRY25150.1"
                     /translation="MHDVKWLREDPAAFDAALARRGVAPCAAELLALDKEWRALETRV
                     QEDQALRNRLSKEIGQRRGPDGKPPAELLDQVTQLKDLLRIREAKLEQVRQALDERLM
                     ELPNALLPEVPDGPDESFNREERRWGTLRNFGFEPRAHEELGAGLGMDFEAAAKLSGA
                     RFVVLRGQLARLERAIGQFLLDLQTEKHGYTEVAVPLLVRDAALFGTGQLPKFAEDQF
                     RTTDGRWLIPTAEVPLTNLVAGLILDETELPFRFTALTPCFRSEAGAAGKDTRGMIRQ
                     HQFSKVELVSITTPEQSADEHERMTACAAAALEALELPYRVMTLAAGDTGFGARRTYD
                     LEVWLPGQGAYREISSCSVCGDFQARRMNARCRPKGERQTRFVHTLNGSGLAVGRTLI
                     AVLENYQEADGSITLPLALRPYMGGMERMIANAQATG"
     gene            complement(11318..11617)
                     /locus_tag="P5558_12370"
     CDS             complement(11318..11617)
                     /locus_tag="P5558_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY25151.1"
                     /translation="MPLYNYLCSDCGPFDAWRAMAEAAHDCACPSCLKPGRRVVSTPH
                     INSMNASLRRALARSEQSSSEPRIVKKAHLAGCGCALCKTGNRRPSVRHRWMIGH"
     gene            complement(11682..12194)
                     /locus_tag="P5558_12375"
     CDS             complement(11682..12194)
                     /locus_tag="P5558_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmiS/UreI family transporter"
                     /protein_id="HRY25152.1"
                     /translation="MLLGFALLYVGAVLFLNGIWLLGRIDDKEIVVINVVSGVVTLCV
                     ALYWAFGAGADGASIRAAALTILFTTTYLWVAYNRVVAVDGRGLGWFSLFVAITVVPV
                     AIQGLSSAQTVTEIWLGLNWVIWAVLWFMYFLLLALQRPILKPTALFTLMAGILTGWL
                     PGFLLLEGAL"
     gene            complement(12277..13503)
                     /locus_tag="P5558_12380"
     CDS             complement(12277..13503)
                     /locus_tag="P5558_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRY25153.1"
                     /translation="MPKTVFSVDLNKAPEDHAIKTHNRWHPDIPMVEMFKPGDVFRVE
                     CHDWTGGQIGNNDSANDVRDVDLTRVHYLSGPFGVQGAEPGDLLVVDILDIGPLPDSE
                     WGFNGVFAKENGGGFLTEHYPQASKSCWDFHGIYTTSRHIPRVRYPGITHPGLIGCLP
                     DHALLAEANRREAELVSSDSGRVPPLAALPYADTALMGQMTGEAAKKAAKEAWRTVPP
                     REHGGNCDIKNLSRGSKVYFPVYVKGGGLSMGDIHFSQGDGEITFCGAIEMAGWIDIG
                     VDIIKGGVAKYGIINPVFKPSPVDPHFDDYLIFEGISVDEHTGEQYYLDAHVAYRRAC
                     LNAIEYLKKFGFSGEQAYMILGTAPVEGRISGIVDIPNVCATLAIPTGIFDFDINPSS
                     TGPTKQVKGADVAKTS"
     gene            13859..14614
                     /locus_tag="P5558_12385"
     CDS             13859..14614
                     /locus_tag="P5558_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY25154.1"
                     /translation="MSGCDHRYRILVVDDDLSSCQLAERYLTGQGHVVVTAHQGAVMR
                     RKLAEERFDLALLDPGFSAGEDGLAVLRYLHTGFGFPIIALSTNATTADRIACLELGA
                     DDFIGKPYEPCELLARMRALMRRLARYVAAPPPAGREPPVGMSFFGWRLDSDGHSLWS
                     PDNEQIRLTGREFQILHALVERRGRILSRDQILDIIADRHWTPYDRSIDVMIAKIRRK
                     LHDDARDTRFIKTIRGIGYMFASQTNQPQSRGY"
     gene            15024..15422
                     /locus_tag="P5558_12390"
     CDS             15024..15422
                     /locus_tag="P5558_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25155.1"
                     /translation="MPTGAGLLRIPRHFPWVVAISATSTPRMLQESLHSPPGCRRKRN
                     ISAGLAHLPQHFPTIEPGKSTIEPTDSTSEPKESTNEPNAGPTNPRARKLLIRLGFLR
                     LSETEPPVERTRDPNIAERTEAGLAFRVEL"
     gene            complement(15454..>16221)
                     /locus_tag="P5558_12395"
     CDS             complement(15454..>16221)
                     /locus_tag="P5558_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HRY25156.1"
                     /translation="ALQPIATALFANSPFTEGKANGFASYRSHIWTDTDPDRCGILPF
                     VFEPGMGFERYVDHVLDVPMYFVYRDGTYIDAAGQSFRDFLEGRLPALPGEKPTMSDW
                     ADHLTTLFPEVRLKRFLEMRGADGGPWRQLCGLPALWVGLLYDRTALEAAWDLCRDWS
                     IAEHQQLRDMTPRLGMRTPFRGGTVQDLALRTMEIAEAGLAARNVMDWDGRDERKYLH
                     ALWEIAKSGITPADEKLARFLGEWQGSVDQVFSGYAY"
BASE COUNT         2535 a         5945 c         5186 g         2555 t
ORIGIN      
        1 caggcgctct tctccggcag ggccggcatg gcgcgcagat tgtggatcaa cgcacccgaa
       61 tcgccccgca ccagggtcag cgccatcgcc tggttgaggt tgaagttcag cgacatgtga
      121 agctcgtcgc cgctctcgtc cccgaagaac gagcgaacgt cgtcatattc gaggttcacc
      181 tcgccgagca tgatggcgcc acctgcccgc ctggccatga atccgctcag gtcacgcagc
      241 cagtcgtgcg gcgtgatctt catctcggtc gcgatgccct cggtctcgag caggaacggc
      301 accgcatcga ccctgaagcc cgagagcccg agctcgagcc agaaccccac gatcttgcgg
      361 atttcggcgc gcaccgccgg gttggcgatg ttgagatcgg gctggtgctc gtagaagcgg
      421 tgcaggaaga agcgccccgc ctgctcgtcc cattcccagt tgctctgctc cgcatcggga
      481 aagacgaggc cctccggccc gccttcgggg atggcgtcac gccacacata ccagtcgtga
      541 tagggcgatt cgcgacccgc cctggcgctt cggaaccacg gatgctctct cgacgtgtgg
      601 ttgaccacca gatcggcaat cacccggatc ccacggtccc gggccgtgcg catcagctcg
      661 acgaagtccc ccagcgtgcc caggcgactg tccacggcgt agtagtcggt gatgtcgtag
      721 ccgtcgtcac ggttgggcgt ggggtagaac ggcatcagcc agagacaggt cacgccgagc
      781 cccgccagat agtcgaggcg gctggtcagc cccgcgaagt cgccgatccc gtcaccgtcg
      841 ctgtcctgga acgtttcgac gtcgaggcag tagaacaccg cgttcttcca ccacagatcg
      901 ctggtccggc tcatgtccat gtggcgtccc tgtcgctgcc ggggagcggg caggttggcc
      961 gcaacgaggt ggcctggcaa tgcctctcgc cgatgccgcc gagccgagga aaccacagtc
     1021 gaatgccgag ctttgggaac ctcggctcga ggtgcctgtt gagccgccag acccatttcc
     1081 aacgccagga gcggatcatg cacggaatca tctatctcgt cgggctcgtc gtcgtcgttc
     1141 tggcggtcct ctcgctcctc ggcctgcgct gagcacgagc gctccgaacc ccctttcaga
     1201 cccggaggat acgatgacgc tcgccaccga atcggcggcc aaccggccgc ccgccacgac
     1261 ctcgactgtt ttcgccgccg agccgcgcgg cagctatgtg gactgggggg ccgtgatcgc
     1321 cggcgcggtg gtcgccgccg ccctgtcgct cgtgctcctc accttcggct cggctctcgg
     1381 cctcgggctc acgtcgctcg agcccggcga gggcgtgtcg ccgctctggc tcgccatcgc
     1441 cgccgccatc tggctgctct gggtgcaggt ctcgagcttc atggccggcg gctacctcgc
     1501 cggccggctg cgtcgcccga tcggcgacgc cgagcccgac gaggtcgatc ttcgcgacgg
     1561 cagccacggc ctcctggtct ggggcaccgg cacgctcgtc ggcgggctga tgctcaccag
     1621 cggcctcctc ggcgccgtgc agacgaccgc ccaggccacg tcgaacgtgg ccggggccgc
     1681 cgtccaggca gcaggcagtg ccgcggacga aggagcctcc tttgccggct acctcaccga
     1741 cacgctgttc cgtgacgcag cgaccggcaa ctcggcccag gccaggagcc aggcccgcag
     1801 cgaagcgggg cgaatcgtca gccaggcact gatcgatggc gaaatccccg ccgacgatcg
     1861 ggctcgtctg gtcgagatcg tggccgagca gaccggcacc gagccggccc aggtcgaggc
     1921 ccgtgtcgcc acgctcgagc agcgcctgca gcagcaggcc gaggacgccg tcgaggcggc
     1981 ggaaactgcc cgccgctggg gcgtgatcgc cgccttcatc gccgctgcga cgatgctggt
     2041 cagcgccgcc ggcgcctact gggccgccac gatgggtggc agccatcggg actccggcct
     2101 cctcaccccc ttctggcgcc gcacccgcta ggtcgccccc accaagctcg aaaggacgcc
     2161 gccatgctct acgtgattct ctggctcctg gggatgccgc tcaccctcgt catcatcctg
     2221 gccattcttc tctagccgaa atgaacgggg cggggtgcct ggcaccccgc cccgctacgc
     2281 ccacgaccgg tcacccgaag gcggtcttca gccgcccttg atcgcgatct tccggacctg
     2341 cttctcggcc gcggcggagc gcggcacctc gatcttcagc acgccattct cgaagctcgc
     2401 ctgcaccttg tcggcgtccg gctcgaaggg cacggcgaac cgccgcatga agctgccccg
     2461 gctccgttcg acgaggtggt agtgcttctt ctcgtccttc tcctccttct cggcccgatg
     2521 ctcggccttc agggtcagaa tgccgtcggt cagatcgagg ctgatgtcct tctgctcgat
     2581 gccgggcagc tcggcgctga tctcgaggcc ccgctcggtc tcggcgacgt cgaccttcgg
     2641 gctcatgagg cccttggcga gcgccggcgc cggccagtcc cgcgagtaac tgtcgaacag
     2701 acgatccatc tcccgccgca tcaggccgaa gggatcggcc tctccgccac ccgcgagacc
     2761 gccgaacccg gcgaacggct gcaacgattt cctggccatg gcatttccac ccgatgatga
     2821 acccacctgc cttccgggga agggaaagct ctcggatcat cacataggag gatgtcccag
     2881 cacgcgcctt gatctggatc agccgcaccc ggcaggcgac gcatttccct gccgccgctc
     2941 atctcccgag cgccgcccgg gtgagatcgg gctggtccgt gaagaacccg tcgaccccga
     3001 ccgcttcgag gcttgcgacc agctccgcca tgtcggccgc ccagccgggc aaccggtccg
     3061 cccgcaaggt gtaggcatga acggccaaac cgcgagctgt cacccgctcc gccagcccgg
     3121 gcgattcgcc tgcgcgatcc agaaccagcc cgatcggcac gccgatcccg tcgaccacac
     3181 cggcaagctc gagaaggccc tcgtcaccca gcagcgcttc gtagtcgctg cccgccgcgt
     3241 ccgcccagtc attgccgccg atgagctgga cgagcggccc cttgtagccg agctcgtcac
     3301 ggattctccg cgtttcggcc cagtcgaagc cctggaggta gagccgcccg tcgccctgcc
     3361 cgccatagcc cgccgcggcc agcgcctcga gcaccacccg gctgatgtcc tgcccctcgt
     3421 cccgatggaa gcccggccgc ttgatctcga catagaggcc gatcgaccgg cccgtgctcg
     3481 cctccagccc ctgcacgaga tcgatcgtcc gggcgagcgt cggcagcccg aaaccgccgc
     3541 tagtgagcgg aaaccgcccg ggaaacaccg cctcgcccgt cttcggatcg acccgctcgc
     3601 cgaccccgag cgtggccagc tcggcccgat cgaagtcgat ggcataatac cgcccgtcgg
     3661 cccgcgcccg gccgggaaag tgctctgcca cgtcggtcgt cgcgtccagc gtcaggtcgt
     3721 gcagcaccac cggcacgccg tcccggctga gcacgaggtc gagctcgacg aaatcggccc
     3781 ccagcccgac cgccagcgcc tgggcctccg tcgtatgctc cggcagatag cccgaggcgc
     3841 cgcgatgcgc gatcaccagc ggttccgccc gggcgagcgt ggcgccgagg agcatcgcca
     3901 gcagtgctcc ggctacgccc agtccgctcg cgcgcatcgc cgcctccttc aggccctgcc
     3961 ccgtccgcca atccggtgta gactggccaa catggtctga aacaggaagt ggcggagccg
     4021 ggcgtggcgc gcaagacgga cgacatcgaa ggcgaggtgg tgacggcggc cctcgacatc
     4081 gccgaagcgg agggctggcc ggccgttcgc ctgcaccgca tcgccgaccg gacgggcctg
     4141 ccgctggagc ggatcggcgc ccggtttcgc gacgtcgacg ccatcgcgga ttgctggttc
     4201 gaccgggcac gcgcagcgct cctggccacg cccgaggcgg acctcgccgg ccgcaccggc
     4261 cccgagcgcc tcgccgtcgc catcgagcgc tggctcgacg ccctcgagcc gcatcgccgg
     4321 gccacgctgc agatgctcaa aggcaagctc tatccctcgc atccgcagca ctgggtgccg
     4381 ctcgtcttct ccctctcccg cctcgtccac gacatcctcg acgtcgcccg cctcgccggc
     4441 gacggccgcc gccgacaggc gcaggaggtg acgctcaccc tcgtcctgct ggccacgctc
     4501 gcccagtggg cccgtgaccg gagcgaaggg caggcgcgaa gccgggccgg cctgcgccgc
     4561 cggctgcgcc gctccgtgct gcaggacgct tgagcattcg cgcggcatcc ctggcgctcg
     4621 tctggctcgc cgcctgcggg ccggccctgc ccgagccgcc gccggcgacc gcggcactgg
     4681 gtgctgccac caccatccag gtcaccgcca atggctggca caccggcatc gtcgtgccgg
     4741 tcgccgccct caccccgggc gtcctgccgg aacgggcgga ctttccggcc gcacgccatc
     4801 tggagctcgg ctggggcgac gccgcctact accccatgcc cgacccgccc cccggcgccg
     4861 tcctccgcgc cgccttcgcc ggcggcccgg cggtgctgca cgtgaccggc catgtgtatc
     4921 ggcccgagcc cggccaggga cgcaccgtcg tcgagatcga cctcaccgcc gccggcctcg
     4981 cggccctcct ccgggccctc gacgcctcct tccagcgacc ggcgagcccc cgccccggcc
     5041 tcgacccggc aagccgcttc taccccgcca ccggccgctt cagcctcttc aacacctgca
     5101 acagctggac cgcccgccag ctcgccacgg ccggtgtcgc cgtcgacccg agcggcgtga
     5161 tcaccgccgg acagctcctc tcgcagctcg acgacctgcc gcaacctctt gtgatgccgg
     5221 cctcttgagc gaggccccgc aactgcgcga ggcggcacgc agcctgccgg tcgccgaggc
     5281 cctccccgcc ctgcgtcgga ccctggccga cgccggcatg gccgtcctga ccgcaccgcc
     5341 cggctccggc aagaccacgc tcgtcccgct cctgctgcag gccgagccct ggctcgccgg
     5401 cggctcgatc ctgatgctcg agccgcgccg cctcgccacc cgccaggcgg ccgagcgcat
     5461 ggccgagctg ctgggcgaga aggtcggcga aacagtcggc tacgccatgc gcttcgagcg
     5521 tcggaccagc tccgccaccc gcctcgaggt ggtcaccgaa ggcctcttcg tccgccgcct
     5581 ccaggccgat ccggggctcg acggggtcgg cctcgtgatc ttcgacgagt tccacgagcg
     5641 ctcgctcgac gccgatctcg gcctcgccct cgcccgcgag gtgcgaagct cgctccgccc
     5701 cgacctccgc ctcctcgtca tgtccgccac gctcgacgcc gaagccgtct cgggcttcct
     5761 cgacgacgcg ccgatcattc gcgccaccgg ccgccagcac cccgtcgccc tgcaccatct
     5821 tccaggcacg ggcccggccg aggcggcgcg caccgccctc gaccgccagc ccggcgacgt
     5881 cctcgtcttt ctgccgggtg cgcgcgagat cgagcggatc gcgggcgagc tcgccggcac
     5941 gccggccgag atccacaagc tgcacggtgc cttgccgcgc gcccggcagg acgcggcgct
     6001 ccgcccgtcg cccggccggc gcaaggtcgt gctcgccacc aacatcgccg agaccagcct
     6061 caccatcgag ggcatcggca gcgtcgtcga tgccggcctc gcccggcgca gccgctacag
     6121 cccgcgcacc ggcatgagcc ggctcgagac cgtccgcgtc agccgggcca gcgccgacca
     6181 gcgcagcggc cgcgccggcc ggctcggtcc cggccacgcc tggcgcctct ggcccgaggc
     6241 cgagacgctg ggcctcgccc ccgccgatcc gccggagatc gccgaggccg atctggcgcc
     6301 cctggtgctc gagctcgcca tctggggcgt gcgcacgccc gacggcctcg ccatgccgac
     6361 cgccccgccc gaaagcgcct ggcgcgccgc ccgcgatctc ctcatggccc tcgatgccct
     6421 ggacgaaggc ggtgatccga caccgcacgg ccgcgccatg gcggccttgc ccgtccaccc
     6481 ccgtctcgcc cacatgctgc tcgaagccgc ccgccacgaa gccgtcggca ccgccctggc
     6541 gctggccgcc ctgctcggcg agcgcgatcc ctggtccggc cggctcggcc ccgacctcgc
     6601 cgaccgcctg gcccgctggc ccgaggcacc gccgacgctc cgccgcacgg cccgccagct
     6661 cgcccggcgg ctgggcgtcg ccgaaacccc gcccgacccg gcaatggccg ggctctgcgc
     6721 cgcgctcgcc ttccccgacc gcatcgccca gcgccgccgc aagggagggg ccgagctgcg
     6781 cctcgtcaac ggcaggggtg cccgcctcgt cgcggccccg gagctcgatc cgttcgactt
     6841 cctcgtcgtc gccgagagcg aggatgccgg cagcgacgcc cgcgtccgcc tcgccgccgg
     6901 cctcgccaaa gacgacctcg accgcctcgc cggccatcgc gtgaccgcca gccgcaccgt
     6961 cgccttcgat gccgagcgcg gcgccatcca ggccgagggg caggcgcggc tcggtgcgct
     7021 ggtgctggcc cgccgcccgg tcgcggtcag cgacgacgag gcgctgccgg tcctgctcga
     7081 cgccatccgc cgccagggcc tggcaatcct gccctggtca ggatcgaccg aggcgctgcg
     7141 ccagaggctc gcctggctgg cgcggatcga gcccgatgcc tggcccccgg tcgacgaagc
     7201 gaccctgctc gccgagctcg ggctctggct cgccccctgg ctcgccggca tccgccgact
     7261 ggccgagctc gacccggcga gcctcgcccg ggccctcctc gcccgcctcg acccggccag
     7321 cagaagccgg ctcgaccgcc tggcgccgac ccactggacg accccgctcg gcagcaccct
     7381 cgccatcgac tacaacagtg atccgccggc catcgcctgc cgcctgcagg agctgctcgg
     7441 cctcgacagc catccgacgg ttgctgccag ccgcccgctt cgcctcgccc tgctctcgcc
     7501 ggccggccgg ccgatccagg tgaccgacga cctgccgggc ttctggcggg gctcctatgc
     7561 cctggtccgc aaggacctcc gcggccgcta cccgaagcac ccatggccag aggacccgct
     7621 tcgagccacc ccctggcgcc cgggcctcgc gcgcggctga gcgctggcgc tcactcgctc
     7681 cgctgggcga tctccaccga gccgtcgcgc agcatcttca aaggatcgat cggcgccttg
     7741 ccgcgcctga tctcgaaatg gagctgcggc tcgaccacct cgccgctggt gccgaccttg
     7801 gccagcggct ggccgcgctg gacgacgtcg ccgcgcgcca cgagcagcgt gtcgttgtgg
     7861 gcataggccg agagataacc gtcggcatgc cgcaccagca gcatccgccc gaaggcggcg
     7921 agatcctcgc cggcatagac gacgaggccg ttctcggtgg cccgcacgag gctgccggcc
     7981 ggggccgcga tgttgatgcc gtcgttctgc cggccgtccg gcttgctgcc gaagcggctg
     8041 atgaccgtgc cgctctcgag cggccagatg aagcccgccc ccgacaaggc cggtgccgcc
     8101 acggccgcga gatcgcgctg gcgcgccgcc tgctcggccg ctgccggtgc tgccggccgc
     8161 gtcgccacgg gcaccacctg gggccgagcc gcgggtgcgg ccgcgaccgt tcgatcggac
     8221 gcgggcgccg cccgtgccgc cacggcggtc tgcggcccgc tcccgcccgg cagccgcacc
     8281 cgtgtgccga ccatgagccg gtccgggtcg atgccgggat tgagggcgat cacgtcgccg
     8341 agcccgacgc ggttcgccgc cgcgatccta gccaggctgt cgcccctgac cacggtgtgc
     8401 gtcgggccgc ccgaaggcgg ggccgaagcc gcctggaccg gcgcaccccg cgccgggttg
     8461 cgggccgcga tcgccgtctc gccgggccgc ggcaggcgca gcacctggcc cacatagatc
     8521 gtgtagggtg ccgagagccc gttcagcgcc acgagatcga ccatgccgac gtgatagagc
     8581 tcggcgaggc cgctcaccgt ctcgcccggc atgaccacat gccggccatt ctcgggcacc
     8641 atccgcgagg tctcgccatt gtcggcgtag atcgccttcc tggcgttctc ccagggcacc
     8701 accccgcccc agtgcacggg ctggtatgcc gtgcagcccg ccattccgag gagtgaaaaa
     8761 aggacgggaa gcgtcgcggc cgaccggccg cgacgtcccc gacagcaaat tgcgcgcagg
     8821 ccccaccaca tgccccacaa ctagctgaaa acagcagtgg catgcaagaa ttcagggcag
     8881 caattcgcgg ttccggcaca agatggtacc gagcgccaag cactctgcag tagaacgcga
     8941 ccttcccggg cttcagaagc cgggcgaggt tgcgcctatt cttgctgtga ttgaaggagc
     9001 ttggtgagct tggcgaagct ctcgcgatgc gtcatgtcga gctggatggg cgtcaccgag
     9061 acccagccgg cccgcaccgc gaacacgtcc gtgtcgtcgg catgctccgc ctcgtcgcgg
     9121 gaaaggccaa tccagacata gggttcgccg cgcgggtcga tgcgctcgat gagctcgtcg
     9181 ccgatcttcg acttgccctg ccgggtcacc ttcacgccct tgacgctgcc ggcgaccaca
     9241 tcggggaaat tgaggttgac cagcacgtcg gtcggccatt ccattgacag catggggcga
     9301 atcacaccgg gtgcccactg ctccgccgtc ttccacttga ccggatgccg ctcgtggcag
     9361 gcctgcgaga aggcgatcgc cggcacctgc aggagcgtcg cctccatgca ggcggcgatc
     9421 gtgccggaat aggtcacgtc ctcgccgaga ttgtggccgt gattgacgcc cgagaggacg
     9481 aggttgatcg ccttgtcctc gaggatgttc tgcagcgcca gcagcacgca gtcggtcggc
     9541 gtgccatcga ccgcgtaatg ccgctcggag agccgccgcg tgcgcaacgg ccggcgcagc
     9601 gtcagcgagt ggccggcgcc gctgtggttg atctctggcg ccaccaccca gacctcgtcc
     9661 gagatctcgc gggcgatcgc ctccatgacc ttgaggccgg gggcattgat cccgtcgtcg
     9721 ttggtgacga ggatgcgggg cttcttaccc ggtggcttga gcattggcga tcatccgttc
     9781 catgccgccc atatagggcc gaagcgccaa gggcagcgtg atgctgccat cggcctcctg
     9841 atagttctcg agcaccgcga tcagcgtgcg gccgaccgcc agccccgagc cgttgagggt
     9901 atgcacgaag cgcgtctgcc gctcgccctt cggccgacag cgcgcgttca tccgtcgcgc
     9961 ctggaagtcg ccgcagaccg agcaggacga gatctcgcga taggccccct gcccgggcag
    10021 ccagacctcg aggtcgtagg tccgccgtgc cccgaagccg gtgtcgcccg cggccagcgt
    10081 catcacccga tagggcagct ccagcgcctc cagcgccgcc gcggcgcaag cggtcatccg
    10141 ctcgtgctcg tccgccgact gctccggcgt ggtgatgctc accagctcca ccttgctgaa
    10201 ctggtgctgg cggatcatgc cgcgcgtgtc cttgcccgcc gcccccgcct ccgagcgaaa
    10261 gcagggggtc agcgccgtga agcggaaggg cagctccgtc tcgtccagga tcaggccggc
    10321 cacgaggttg gtgagcggca cctcggccgt cgggatcagc cagcgaccgt cggtcgtcct
    10381 gaactggtcc tcggcgaact tcggcaactg ccccgtgccg aagagcgccg cgtcgcgcac
    10441 caggagcggc accgccacct cggtgtagcc gtgcttttcc gtctgcaggt cgaggaggaa
    10501 ctggccgatc gcccgctcga gccgggcgag ctggccccgg agcaccacga agcgcgcgcc
    10561 actgagcttc gccgccgcct cgaaatccat gccgagcccg gcgcccagct cctcgtgcgc
    10621 ccggggctcg aagccgaagt tccgcaaggt cccccagcgc cgctcctcgc ggttgaagct
    10681 ctcgtccggc ccgtccggca cctcgggcag gagcgcgttc ggcagctcca tcagccgctc
    10741 gtcgagggcc tggcgcacct gctccagctt cgcttcgcgg atcctgagca gatccttgag
    10801 ctgcgtcacc tggtcgagca gctccgccgg cggcttgcca tccggcccgc gccgctggcc
    10861 gatctccttc gagagccggt tcctgagcgc ctggtcctcc tggacccgcg tctccagcgc
    10921 ccgccactcc ttgtcgagcg ccagcagctc tgccgcgcac ggcgccaccc cgcgccgcgc
    10981 cagcgcagca tcgaacgccg ccgggtcctc ccgcagccat ttcacgtcat gcatggagag
    11041 cggaatcttt cacacgaatg tccgccaacc ctctagcgga cacccggcac caccgcaacc
    11101 gcgggggtgg ggtggcgaag cccctgcaac ggcatcaggt gccgttaacg cgggagccga
    11161 cgtgcggggt ggcgccgaaa ttcacgcatc cgggctaccg caaccagaat aggctgccgt
    11221 ggatgtcggc ccaacgaatt gccacgccag aagatctggt ggacgcgtcc atccttctcg
    11281 agaaggctgg gcacccgcgc ctgacggcgc gcttgcatca atgaccgatc atccacctgt
    11341 ggcggaccga gggtcgtctg ttcccggtct tgcacaaagc gcaaccgcag cccgcgagat
    11401 gggctttctt cacgatcctg ggctcgctgc tgctctgctc ggaacgcgcg agcgcgcggc
    11461 gcaggctggc attcatcgag ttgatatgcg gtgtcgacac cacgcggcgg ccgggcttga
    11521 ggcagcttgg gcaagcgcag tcgtgcgccg cttcggccat cgcccgccat gcatcgaacg
    11581 ggccgcaatc gctgcaaaga tagttgtaga gcggcatcgc aacctccctg aagatggcgg
    11641 acggaggagc ctcgagaatg gcttgccgaa ggcaggcacc ctcacaaggc tccttccagc
    11701 aagaggaatc ccggcaacca acccgtgagg atgccggcca tgagagtgaa aagggcggtg
    11761 ggcttgagaa tggggcgttg aagggcgagg agcaggaagt acatgaacca gagaaccgcc
    11821 cagatgaccc agttcagtcc caaccatatt tccgtgacgg tttgggcgga cgacagccct
    11881 tggatggcga cgggcacaac ggtgatggcc acgaacaggc tgaaccagcc gagaccgcgt
    11941 ccgtcgaccg caacgacccg attgtaagcc acccagagat aggtggtggt gaacaggatc
    12001 gtcagcgccg ccgctcttat cgaggcgccg tcggcgccgg caccgaacgc ccagtaaagc
    12061 gcgacgcaaa gcgtgacgac acccgagacg acgttgatca cgacgatttc cttgtcgtcg
    12121 atccttccca gaagccagat cccgttcagg aacagaaccg cgccaacgta gagcaaggca
    12181 aagccgagca acatgaccgc ccctcgtatg ggcgccccgg ccggagcctc tcgtccggcc
    12241 ggggcgcgat tcgatgtcaa gtgcgcgttg cggccatcag ctcgtcttcg cgacgtcggc
    12301 acctttgacc tgtttcgtcg gaccggtgga ggacgggttg atgtcgaagt cgaaaatgcc
    12361 cgtcggtatg gccagggtgg cgcagacgtt ggggatgtcg acgatgccgg aaatgcgccc
    12421 ttcgaccggt gccgtcccca ggatcatgta ggcctgctcg ccggagaagc cgaacttctt
    12481 gaggtattcg atggcgttca ggcaggcacg tcgataggcg acatgtgcat cgaggtaata
    12541 ttgctctccg gtgtgctcgt cgaccgatat gccctcgaag atcagatagt cgtcgaagtg
    12601 cgggtccacc gggctcggct tgaagacggg attgatgatg ccgtacttcg cgacaccgcc
    12661 cttgatgatg tcgacaccga tgtcgatcca gcccgccatc tcgatcgcgc cgcagaaggt
    12721 gatttcgccg tcaccctggg aaaaatggat gtcgcccatg gaaagcccgc cgccctttac
    12781 gtagacgggg aaatagacct tcgagccgcg cgagagattc ttgatgtcgc aattgccgcc
    12841 gtgctcgcgt ggcggcacgg tgcgccaggc ttccttggcg gctttcttgg ccgcttcacc
    12901 cgtcatctga cccatcaacg ccgtgtccgc atagggcaag gcggccagtg gcggcacccg
    12961 gccggaatcg ctcgagacga gctcggcctc gcggcggttg gcctcggcga gcagcgcatg
    13021 atcgggcaga cagccgatca gaccgggatg ggtgatgccc gggtaacgga cgcgcgggat
    13081 gtgccgcgac gtggtgtata tgccatggaa atcccagcag gacttgctcg cctgcggata
    13141 gtgctcggtc aggaagccgc cgccgttctc cttggcgaac acgccgttga agccccactc
    13201 ggaatcgggc aggggaccga tgtcgagaat gtcgacgacc agaagatcgc ccggctcggc
    13261 accctgcacg ccgaacgggc cgctcaaata gtgaacgcgg gtcagatcga cgtcacgaac
    13321 atcgttggcg ctgtcgttgt tgccgatctg cccacctgtc caatcgtgac attcgactcg
    13381 aaagacgtcg cctggcttga acatctcgac catcgggata tccggatgcc agcgattatg
    13441 ggtcttgatc gcatgatctt caggggcctt gttgagatcg accgagaaca cggttttcgg
    13501 catcgatttt cctccgcttt tgcaagtttt tcaagccgtc gatctcgacc ctgctcgtat
    13561 ctcgtcggac tactccgagc attcgagaac ttctcggaca ggctccaagg ctagccaacg
    13621 aacgtaattt ccatgtgtcg aaggagcccg ctattgtaaa cttttgtaac cgacgactct
    13681 gcacttctgg tcacggcgaa gcggtcatcg cctcgaaata gaattttgaa aaaaggctca
    13741 acctgacgtt gctttagatt gcaggatcaa gggggctgct ggcaaaacgg agggccctct
    13801 tcgagggcga catctttccg actcgactga ttccagcctg gcggctagcg tgatattcat
    13861 gtcgggttgc gatcacagat accgtattct cgtcgtggac gatgaccttt catcgtgcca
    13921 gctcgccgag aggtatctga caggtcaggg gcatgtcgtc gtcacggctc accagggagc
    13981 agtgatgcgg cggaagctgg cggaggagcg attcgacctc gcgctcctcg accctggctt
    14041 ttcggccggc gaggacggtc tggccgtcct gcgctatctg cacaccggtt tcggctttcc
    14101 catcatcgcc ctgtccacca acgccaccac cgcggacagg atcgcttgtc tcgagctggg
    14161 tgcggacgac ttcatcggca agccctacga gccgtgcgag ctcctggcgc ggatgcgggc
    14221 tctcatgcga cgtctcgcca ggtacgtcgc ggcgccgccg ccggccgggc gcgagcctcc
    14281 ggtgggcatg agctttttcg gctggcggct cgattcggac ggacactcgc tctggtcgcc
    14341 ggacaacgag cagatccggc tgaccggccg ggagttccag atcctgcacg ccctggtcga
    14401 acggcgcgga cgaattctct cgcgcgacca gatcctcgac atcatcgctg acagacactg
    14461 gaccccctat gaccgcagca tcgatgtaat gatcgccaaa attcggcgca agttgcacga
    14521 cgatgcacgc gacactcgct tcatcaagac aattcggggc atcggctaca tgttcgcctc
    14581 ccaaacgaac caaccgcagt cgcgtggata ctgagcacgc ctccgatgcg ggacgcttcc
    14641 cttcttcgga ttttcgggtc cacaaaactc gattggccgc tcggctttcc ggtccacgag
    14701 atccaatcga ccgttcgccg agtcggtcct cgccttgcgc tcgaacatgc tcgagatgca
    14761 taatggcgac ggacgcgagt gagggcgcgc tcgagtcccg actggtattt tatcgacaag
    14821 tctggccgcc caacggccag ccaaccaacc gcgatagaat tctgtgtggc cccccaggcg
    14881 cccggggagt cagtccatgc ggactatcga catcgtcatg gtggtcttcg cggccatcag
    14941 cgcgatcgcc gccatcctgc aggtcctgct gatgctcggc atcgcgtagg ctttcgaggc
    15001 gcgtcgccag gcgcgcctct tccttgccga ccggcgccgg gttgttgcgc ataccgcgac
    15061 attttccttg ggttgtcgca atttctgcaa catccacccc ccgcatgttg caggaatcgc
    15121 tacattcgcc acccggatgt cgcagaaaac gcaacatttc ggccgggttg gcgcatctgc
    15181 cgcaacattt tccgacgatc gaacccggca aatcgacgat cgaacccacg gattcgacga
    15241 gcgaacccaa ggaatcgacg aacgaaccca acgccgggcc aacgaaccca agggcgcgca
    15301 agttgttgat ccggctcggc tttttgcgcc tttcagagac cgagccaccg gtcgagcgaa
    15361 cccgagaccc gaatattgcc gaacgaaccg aagccgggct tgccttcagg gtcgagctct
    15421 gataggaata ttctctgcca acaatggcgc cgctcagtag gcataccccg aaaagacctg
    15481 atcgaccgag ccctgccatt cgcccaggaa ccgggcgagc ttctcgtcgg ccggggtgat
    15541 gccggacttg gcgatctccc agagcgcgtg gaggtacttg cgctcgtcgc ggccgtccca
    15601 gtccatgacg ttcctggcgg cgagcccggc ctcggcgatc tccatggtcc ggagcgccag
    15661 gtcctggacg gtgccgccgc ggaacggggt ccgcatgccg agccggggcg tcatgtcgcg
    15721 gagctgctgg tgctccgcga tggaccagtc cctgcagagg tcccaggcgg cttcgagcgc
    15781 cgtccggtcg tagagcaggc cgacccagag cgccggcagg ccgcagagct ggcgccaggg
    15841 gcccccatcg gcgccgcgca tctcgaggaa gcgcttgagc cgcacctcgg gaaagagggt
    15901 cgtcaggtga tctgcccagt cgctcatcgt gggcttctcg cccggcaagg ccggcaggcg
    15961 accctcgagg aagtcgcgaa aggactggcc cgcggcgtcg atataggtgc cgtcgcgata
    16021 gacgaagtac atgggcacgt cgagcacgtg atcgacatag cgctcgaagc ccatgcccgg
    16081 ctcgaagacg aagggcagga tgccgcagcg gtcggggtcc gtgtcggtcc agatgtggct
    16141 cctgtagctg gcgaagccgt tggccttgcc ctcggtgaag ggcgagttcg cgaagagggc
    16201 ggtagcaatg ggttgcagcg c
//
LOCUS       DAOTFI010000109        16122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4360_length_16122_cov_8.176075, whole genome shotgun sequence.
ACCESSION   DAOTFI010000109 DAOTFI010000000
VERSION     DAOTFI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 16122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16122
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_16122_cov_8.176075"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="P5558_12400"
     CDS             <1..677
                     /locus_tag="P5558_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HRY25157.1"
                     /translation="EWDNFGLVTVDSPMIYSIVDVLLGGRRGTVPMRIEGRPYTTIET
                     SLVERLIRLVLQDLSSAFQPITDVLFRFERMETNPRFAAIARPGNACIVFKMRIDMED
                     RGGTIEFLVPYATLEPARDLLLQMFMGEKFGRDSIWENHLAREILVTDVELEAVLEVK
                     TVSLAFVADLEVGSVLRLRSRPETDITLRCGQVEMLRGKVCQRGDTMCVQVEEKIERP
                     RRRTDD"
     gene            670..1239
                     /locus_tag="P5558_12405"
     CDS             670..1239
                     /locus_tag="P5558_12405"
                     /inference="COORDINATES: protein motif:HMM:NF039465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HRY25158.1"
                     /translation="MIDLALQVVLVVLLLLTITWCMIVHLRLRRLRTEGGEMQAFIAA
                     LGEATTRAEAAISLMRDTGREIERAAIEQERRARECRGELGRLVENAARVTQRLTAAR
                     EQGAARMAELRTRDDIADEPRQRLVEPSPDASPRADLAPAAPAPAPASPDPERAGRPR
                     PARAKGGQPEARLDGLLHGELLEALQALR"
     gene            1236..2042
                     /locus_tag="P5558_12410"
     CDS             1236..2042
                     /locus_tag="P5558_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25159.1"
                     /translation="MIPWPSRRMAGAVRHVRRRLWQSWEYALAWRAGPARLSLPLVTA
                     AAACLALVALSVELVSDLGKTRAPPPSGPAVDAVAEESFLRSASVAFDWDLVEPAAGP
                     VATDPPPRPGTPSGDPEIAAPNSLPVIPEAPPELADVALELQQRREQLLELEARLAIR
                     EAALRAAEADLAAQLDRLEALRGELQELVGLADAEEEARQQQLVKVYESMRAKSAAQI
                     FDRLDLAVLLPVAKRMKEVKMAAILAAMNPDRARIVTTELAREQSLPTLH"
     gene            2161..2847
                     /locus_tag="P5558_12415"
     CDS             2161..2847
                     /locus_tag="P5558_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HRY25160.1"
                     /translation="MTPVAEPPDRSPRQFSAAEIGAIAHLYRGEVYRSTIWRTRLDNT
                     TNWSVVTLGIALSITFSSANASPLPLVLVGILVSLFLVLEARRYRYFNVWRARCRWME
                     THFYAPLLEDGDLHTEDGWQQTLARDYLAPRYHIGFMRALGRRLRRNYVWILLIQTVA
                     YLSKIIIHPSAATTLGELLDRAAIGPLPGAVVLACGILYNLAWIGTALVTLRLDQAAH
                     ATRRDTTAMG"
     gene            complement(2913..4211)
                     /locus_tag="P5558_12420"
     CDS             complement(2913..4211)
                     /locus_tag="P5558_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY25161.1"
                     /translation="MIVTSLVLLAIILLLLASGIWVAISLLAAGVVGLAFFTAAPAGS
                     LLASAMWDASWSWALTALPLFVWMGEILTRSRLSRDMFAGVSPWVGRLPGRLLHVNIL
                     SCGIMAAVSGSSAVTAATIGRMSLPELAKRGYDERMMIGTLAGSGTLGLLIPPSIVMI
                     VYGVTAQQSVARLFIAGILPGLLLVALFMGYVAVWAMLHRGRMPIADPPVPLRRKIWE
                     ARRLIPVLLLIVLVIGSIYGGIATPTEAATIGVVGSLILAALGRTLTLASFVDGLMAA
                     TRMSCMIALIIAAAACLTIAVGFLDIPRALASWVDALGLSSWGLLAVLAVCILVMGCF
                     LEGISIIVLSASVMLPMVQAAGIDLLWFGIFVVILIEAAQITPPVGFNLFVLQSLTGR
                     DIWRVTAATIPFFLLLMLLLVLITVAPGIVTWLPERMIAR"
     gene            complement(4233..4745)
                     /locus_tag="P5558_12425"
     CDS             complement(4233..4745)
                     /locus_tag="P5558_12425"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY25162.1"
                     /translation="MIRRTLDALYLWSGAAAAVLLALIAVAIIVQVAGRSLGYVVDST
                     EIAGFFMAASSFFALAHTFKRGTHVRVTLLVERLTGGGRRILEAWCCLAAAGFVGFLA
                     WWALDLVAQSWRFGDISPGLLAIPFWIPQAAMAFGLLLLTLALVDEAVGIVRGREPAY
                     ASGEDTALDD"
     gene            complement(4789..5772)
                     /locus_tag="P5558_12430"
     CDS             complement(4789..5772)
                     /locus_tag="P5558_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY25163.1"
                     /translation="MSRSTTTIAAAALMAFATPALAETSWIMASGYPEDSFFTKNIRL
                     FIEDVKEATGGELVIDLRSNDSLIKHDAIKRAVQSGQVQAGEIRLSVYGNEDIMYGLD
                     SIPNLVTSYDEAWKLMEAQDVYLSKLFEENGMRIITYVSWPGQGFYTAEPVTSLADLK
                     GQSLRIYSPQTQQMGEMLDMNATILPFAEVPQAFSTGLIEALWTSAQTGTDVQAWDYV
                     DHFTYTGSLHTKNAIIVNGDAFDALTEDQQQAVLAAGKKASERGFELSKEASREREQV
                     LIDHGMTVTEAPEDVMDKMREIGLTMVEEWRGEANPDQVAVLDAYLASVGR"
     gene            complement(5826..7475)
                     /locus_tag="P5558_12435"
     CDS             complement(5826..7475)
                     /locus_tag="P5558_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY25164.1"
                     /translation="MAKGLDDIHMQVMWNRLISVVEEQALTLVRTAFSTSVREAGDLS
                     AGVFDVAGRMLAQAVTGTPGHVNAMAESVGHFITDIGPGNILENDVYITNDPWKGTGH
                     LHDVTVVTPVFRKRALIGYFACTAHVVDVGGRGFGPDGRDVHEEGLFIPIMKLFREGA
                     VNRDLILIVRANVREADKVVGDLYALAACNETGMKRLFAMMDEFGFDDLDGIGSFILE
                     RSDRATRALIAELPRGRHTNAMTLDGYDEPVHLAVTLEIEEDRIKADFTGTSEVSRWG
                     INVPLVYCKAYACYGLKCVVAPEIPNNAASLAPFEISAPPGCILNAEPPAPVSVRHVL
                     GHFVPDLVMGALAGALPDRVPAEGSGALWNIQFSVRPLPSNTTGRPAEMLLFNSGGTG
                     ARPTLDGLNATAFPSGVMTMPAEATEQVGPLVVWRKELRDGSGGAGRHRGGLGQVIEI
                     AAANGYEIAFNAMFDRTNHPARGRKGGRPGRAGTVGLDDGTKLAPKGRQSVPVGRRLV
                     LGMPGGGGHGDPRERAPASIEADLAHDYIEAAQRADYEPRN"
     gene            complement(7475..9574)
                     /locus_tag="P5558_12440"
     CDS             complement(7475..9574)
                     /locus_tag="P5558_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRY25165.1"
                     /translation="METTAAAARARAGIDIGGTFTDIAIEANGVVHSAKVLTDRERPE
                     RGVLQGLQAAARTAGVAPGELALLIHGTTLATNALIERRGARAGFVTTRGFRDVLEMR
                     TESRFEQYDLNIQLPPALVPRIDRLTLAERIDAQGRVLVAPEQSEIEALCATIEERGY
                     ASVAIGFIHSYMNDAHEQRMREALAKRLPGLEISISSEVSPQMREYERFNTVCANAYV
                     KPLIKSYLRRLVDELGAIGVGCPLFLMHSGGGIIGVESAAEFPVRLVESGPAGGAVFA
                     ADIARRYGLDRVLSYDMGGTTAKICLIEQQTPKTARTFEVARTWRFKKGSGMPISIPV
                     VEMVEIGAGGGSIASVDLMGRIRVGPRSAGSEPGPACYGRGGSEPTVTDADLLLGRID
                     PHDFAGGSMTLDSAAANGAVERAVGAPLGLDGEAASYGIAEMVDENMANAGRVHAVEN
                     GKELADFTMICFGGAGPLHAARLCEKMGIDRFLVPPGAGVGSAIGFLRAPFGYESVRS
                     RLTPLHDFDADAVNALLDAMRAEAAAFVAQGGGDAEQLTECTAYMRYAGQGWEIPVAI
                     EPGGYAAGDERKVARAFAEAYTRFFGRPVEGAMIEIVSFALKVATRLPPTPTLELARE
                     GRPARPRGSRRLFEPGEGRFVDAAVFARDALEAGAELAGPAVVVEDETATILTTGSLA
                     VVQADGCLLVLRRKGEG"
     gene            9757..13254
                     /locus_tag="P5558_12445"
     CDS             9757..13254
                     /locus_tag="P5558_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY25166.1"
                     /translation="MLRITREDIDDAVGDVFADRGEAYFARGKVQGLRYEEGIGRLTG
                     RVQGSTRTPYRVDIHLEPRGQGGLEVEGVCSCPVGINCKHVAALLFAAQARLPEQRAV
                     EVVAAPSAGAPRPEEGPDPRPEDGLDEPARRWLAALRAAQADDANSFADSVQNRVIYV
                     LRVEPGRTWIEPVKVRLTKAGGYAIERRDYAYQLYSSAPRPKFITPADHNILQRIHGL
                     FAYDRSGLEQRLESGALTARQITGLILDILATGRCCWAGPDPARLLAPGAPRPGTARW
                     TADERGDQSLCFVADDGGPAFDGVIPIDPPWYVDLEAGVCAPIETGLDERLAGTLARA
                     PRLAPAATVRLREAMAADLPAGLSIPLPAAFKRVRAEAVPPVPVLRLGCVGLKLEMPG
                     RRHYHWYARPTVAVDLPVFRLAFDYGGVIVEPSSKGEPERREGQRIVVTPRDARAEAR
                     AQSRLDRLGLVRLGDRRSPLKGVTAEHAEARVPEFDDVAVAELLEESVLDQSAYLAIA
                     GDGLPRLQTEGWRIEEDESWPFRLVAEPVEWQAEIAEGEGWGWFDLRLGIDIGGERFD
                     LLPFLSGLIAQLPRSTDGGPPDDETLEAIFTDMVLYPRLADGRLLPLAGERLLLPVKA
                     LFALLAREPATDAVARVHVSDLDALVDEVVLAWQGRERLDALRAAIASLRAAPEIDPP
                     AGFGAELRPYQRAGLAWLQALRQAGFGGILADDMGLGKTVQALAHLAIEKARGRLSQP
                     CLLVAPTSLMGNWWREAARFAPELRLVLWHGGDRTMRAADLDGADLVLTTYALTRRDE
                     ALLLGRRWSMLILDEAQAVKNPRSATARIIGRIEAGQRLALTGTPLENNLDELWSLFH
                     IIAPGLLGDLKRFRSSFRAPIEKDRDASANARLAARIRPFMLRRTKEAVASELPPKTE
                     IVDWVELAGAQRDLYETVRLMMDAKVRSSVERMGLQRSHIAILDALLKLRQVCCDPAL
                     VKIDGGRRVQGSIKRERLLDLLETLLAEGRRVLLFSQFVEMLKLIQGDLDARGIHRSL
                     LTGRTRDRQAEIDRFEGGQTRLFLISLKAGGTGLNLTSADTVIIYDPWWNPAVESQAM
                     DRAHRIGQTKPVFVYKLVAEGTVEAVIQEMQARKRALAQGVYGDDAERPLDLDEEDLM
                     QLLAPIGGDKRASPGGSRP"
     gene            complement(13340..13879)
                     /locus_tag="P5558_12450"
     CDS             complement(13340..13879)
                     /locus_tag="P5558_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25167.1"
                     /translation="MQTLKLTAAALTLSLLASALSPHAMAQQGGVQIGGNANNTTLLN
                     QNTNAAIGLGAKANTRVGSIQDTTVGGNLNNTTLVNQNTNAAIGLGSKASTQIGAVTG
                     SDVGGNLNNTTLVNQNTNAAIGLGSKAGSAIGTVNNATVGGNVNNTTLVNQNTNAAIG
                     LGTKACSEIGSIGKSDVCG"
     gene            14085..15065
                     /locus_tag="P5558_12455"
     CDS             14085..15065
                     /locus_tag="P5558_12455"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY25168.1"
                     /translation="MRALVGALMLAGAGILGLAPADHATAQQTLRFAHFSSTEDPNHV
                     AALRFKELLEAETDGRYTVDIYENSQLGGEVDVVEGIQLGTVDVSPPSAAVLANVVTE
                     MNILNMPFLFRDWPHYEAVLEGEFFDAIVEVAAADGIRILGFMTTGPRHIMTKFPVES
                     MADLAGKKIRTVQNPVHVATFTAFGANATAIAYPEVYGALQTGVVDGGDAANTNYYTA
                     KFYEVAPYWAQVSWLFYSNPLVMSEDAFQALSPEDQEIFQRVGRQVGREQIANWKASD
                     AGLLDELKKAGVTVTTPPREPFQEAVGEVYDTFLASDFEKEWLGRIQDTE"
     gene            15091..15597
                     /locus_tag="P5558_12460"
     CDS             15091..15597
                     /locus_tag="P5558_12460"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY25169.1"
                     /translation="MPARLLTALALVRRVFRLATILCLVVMTLSITVQVVGRYLLSFP
                     IADAVEISTFAQVWLVLLGAGIACRHDALFTVDLFGQRLPPVLHRLRGWLILACGSVF
                     LGLLLWGSFSLLGLGLRQRAPTLQIPMIWIYAALPIGFLYFWIELAVRSLDLEPALSA
                     EARNGETR"
     gene            15594..>16122
                     /locus_tag="P5558_12465"
     CDS             15594..>16122
                     /locus_tag="P5558_12465"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY25170.1"
                     /translation="MIIVVLAFLVLMALGVPIVLALGSPVALYILISGDVPIVVIAHR
                     FYSGLNNFTLLAIPFFVLAGLLMEAGGLSRRIVDFASSLVGWIQGGLLMVAVVASTAL
                     AAMSGSGSADAAAVSAVLQPELRRRHYEIDFAAALIAASATLAQIIPPSLMLVIIALQ
                     NNLSVGALFLGGVLPG"
BASE COUNT         2440 a         5543 c         5691 g         2448 t
ORIGIN      
        1 tcgagtggga caatttcggc ctggtcaccg tcgacagccc gatgatctac tcgattgtcg
       61 acgtgctgct cggcggccgt cgcggcacgg tgccgatgcg catcgagggg cgcccctaca
      121 cgaccatcga gacctcgctg gtcgagcggc tgatccgcct cgtgctgcag gacctgagca
      181 gcgccttcca gccgatcacc gacgtcctgt tccgcttcga gcgcatggag accaacccgc
      241 gcttcgccgc gatcgcccgg ccgggcaatg cctgcatcgt cttcaagatg cgcatcgaca
      301 tggaggatcg gggcggcacg atcgagttcc tggtgcccta cgcgaccctc gagcccgccc
      361 gcgacctgct cctgcagatg ttcatgggcg agaagttcgg ccgcgactcc atctgggaga
      421 accatctcgc ccgcgagatc ctggtgaccg atgtcgagct cgaggcggtg ctcgaggtga
      481 agacggtgag cctcgccttc gttgccgacc tcgaggtcgg ctcggtcctc aggctgcgct
      541 cgcggccgga gaccgatatc acgctgcgat gcgggcaggt cgagatgctg cgcggcaagg
      601 tgtgccagcg cggcgacacc atgtgcgtcc aggtcgagga gaagatcgag cggccgagga
      661 ggcgcaccga tgattgatct ggccctgcag gtcgtgctcg tcgtcctgct cctgctgacc
      721 atcacctggt gcatgatcgt ccatttgcgc ctccgccggc tgcggaccga gggcggcgag
      781 atgcaggcgt tcatcgcagc gctcggcgag gccaccaccc gggcggaggc agccatcagc
      841 ctgatgcgcg acaccggccg cgagatcgag cgcgccgcca tcgagcagga gcgtcgggcg
      901 cgcgagtgtc gcggcgagct cggccggctc gtggagaacg cagcacgcgt cacgcagcgc
      961 ctgacagcag cccgcgagca gggtgccgcc cggatggcgg agctgcgcac ccgggacgac
     1021 atcgcggacg agccacgcca gcgcctggtc gaaccgtcgc cggacgcttc gccccgggcc
     1081 gatctggcgc cggccgctcc ggctccggct ccggcatcgc cagacccgga gcgcgccgga
     1141 cggccacgcc cggccagggc caagggtggc cagccagagg cccggctcga tggtctcctg
     1201 cacggcgagc tcctcgaggc cctgcaggcg ctcagatgat tccctggccg tcgcggcgca
     1261 tggcgggggc ggtgcggcac gtgcggcgcc gcctctggca gtcctgggaa tacgccctgg
     1321 cctggcgggc cggcccggcg cggctttcct tgccgttggt gacggccgcg gcagcctgcc
     1381 tcgccctcgt cgccctctcg gtcgagctgg tctcggacct tggcaagacg cgtgcgccgc
     1441 cgccttccgg gccggcagtc gacgctgtgg ccgaggagag cttcctgcgg tcggcgtctg
     1501 tcgctttcga ctgggacctg gtcgagccgg ccgcgggacc ggtcgcaacc gatccgccgc
     1561 cccgacccgg caccccttcc ggggatcccg agattgccgc accgaacagc ctgccagtca
     1621 taccggaagc gccgccggag ctcgccgacg tggccctcga gctgcagcag cggcgtgagc
     1681 agctgctcga gctggaagcg aggctcgcca ttcgcgaggc ggcgctgcgc gcggccgagg
     1741 ccgatctcgc cgcccagctc gaccggctcg aggcgctccg tggcgagctg caggagctgg
     1801 tcggcctcgc cgatgccgag gaggaggcgc ggcagcagca gctggtcaag gtctacgaga
     1861 gcatgcgtgc gaaatccgcg gcccagatct tcgaccggct cgatctcgcc gtcctcctgc
     1921 cggtcgccaa gcggatgaag gaggtgaaga tggcggcgat cctcgcggcc atgaaccccg
     1981 accgggcacg gatcgtcacg accgagctcg cccgcgagca gagcctgccg accctccact
     2041 gagctcgcca tcgagaccgt ccgatccagc ctccgcacct cgcgctgccg ctacgagata
     2101 tgcggccggc cccgaaccgc tctaggatga gggcggacaa ggcgacggga ggcatgcctg
     2161 atgacacccg tggcggagcc gccggaccgg tcgccgcgcc agttcagcgc ggcggagatc
     2221 ggtgccatcg cccatctcta ccggggcgag gtctatcgca gcacgatctg gcgcacccgg
     2281 ctcgacaaca ccaccaactg gtcggtggtg acactcggca tcgccctttc catcaccttc
     2341 tcctcggcca acgcctcgcc cttgccgctc gtcctggtcg gcattctcgt cagcctcttt
     2401 ctcgtgctgg aggcccgccg ctatcgctac ttcaatgtct ggcgggcgcg ctgccgctgg
     2461 atggagacgc atttctacgc gcccttgctc gaggacggcg accttcacac ggaggacggc
     2521 tggcagcaga ccctggcgcg cgactacctg gccccgcgct atcatatcgg cttcatgcgg
     2581 gctctcggcc ggcggctgcg ccgcaactat gtctggatcc tgctgatcca gaccgtcgcc
     2641 tatctcagca agatcatcat ccatccctcc gcggccacca ccctgggcga gcttctcgac
     2701 cgcgccgcca tcggccccct gccgggtgcc gtcgttctcg cctgcggcat cctctacaac
     2761 ctcgcctgga tcggcacggc gctggtgacg ctgcgtctcg accaggccgc ccacgcgacc
     2821 aggcgcgaca cgaccgccat gggctgacca cgaaggccga gggcaggcac accgccatgc
     2881 catggagcgg cgcacccgcg acaccggctc cctcaccggg cgatcatgcg ctccggcagc
     2941 caggtgacga tcccgggtgc cacggtgatc agcacgagca gcagcatcag gaggaggaag
     3001 aatggtatcg tcgcggcggt gacgcgccag atgtcgcggc cggtgagcga ttgcaggacg
     3061 aagaggttga agccgaccgg cggggtgatc tgggcggcct cgatgaggat gacgacgaag
     3121 atgccgaacc agagcaggtc gatgccggct gcctggacca tcggcagcat gaccgacgcg
     3181 ctgagcacga tgatggagat gccttcgaga aagcagccca tgaccaggat gcagaccgcg
     3241 agcacggcga gcagacccca ggacgaaagg ccgagtgcgt cgacccagga tgccagggcc
     3301 cgggggatgt cgaggaagcc cacggcgatg gtcaggcagg cggcagcggc gatgatcaat
     3361 gctatcatgc aggacatgcg ggtggccgcc atcaacccgt cgacaaagct ggccagcgtc
     3421 agcgtgcggc cgagcgcggc gaggatcaac gagccgacga cgccgatggt ggcggcctcg
     3481 gtcggcgtgg cgatgccgcc atagatcgag ccgatcacca gcacgatgag caagaggacg
     3541 gggatcagcc ggcgggcctc ccagatcttg cggcgcagcg ggaccggcgg gtcggctatc
     3601 ggcatccggc cgcggtgcag catggcccag accgccacat agcccatgaa gagggcgacc
     3661 agcaggaggc cgggcaggat gccggcgatg aacaggcggg cgacggattg ctgcgccgtg
     3721 acgccgtaga cgatcatgac gatcgagggc gggatcagga ggccgagcgt gccggagccg
     3781 gccagcgtgc cgatcatcat ccgctcgtca tagccgcgct tcgccagctc cggcaggctc
     3841 atgcggccga tggtggcggc ggtgacggcg gacgagccag agaccgccgc catgatgcca
     3901 cagctcagga tgttgacgtg caggagccgg cccggcagcc ggccgaccca gggcgagacg
     3961 ccggcgaaca tgtcccgcga gaggcgcgag cgggtgagga tctcgcccat ccagacgaag
     4021 agcggcaagg ccgtgagcgc ccagctccag ctcgcgtccc acatggccga ggcgagcagc
     4081 gagcccgccg gagccgcggt gaagaaggcg aggccgacca cgccggcagc gaggagcgag
     4141 atcgccaccc agatcccgct cgcgagcagc agcaggatga tggcgagcag gaccagggag
     4201 gtgacgatca tgggctgctg ccggggcgac ggtcagtcat cgagggccgt gtcctcgccc
     4261 gaggcatagg ccggctcacg cccacggacg atgccgaccg cctcgtcgac gagggccagg
     4321 gtgagcagga gcaggccgaa cgccatggcc gcctgcggga tccagaaggg gatggcgagc
     4381 aggcccgggc tgatgtcgcc gaagcgccag gactgggcga cgaggtccaa ggcccaccag
     4441 gcgaggaagc cgacgaagcc ggccgcggcg aggcagcacc aggcctcgag gatacgccgg
     4501 ccgccgccgg tcaggcgctc gaccagcagg gtgacgcgga catgggtgcc gcgcttgaag
     4561 gtgtgggcga gggcgaagaa ggacgaggcg gccatgaaga agccggcgat ctcggtggaa
     4621 tcgacgacgt agcccaagct acggccggcg acctggacga tgatggcgac ggcgatcaag
     4681 gccagcagca ccgcggccgc cgcaccgctc cagaggtaga gcgcatcgag ggtgcggcgg
     4741 atcatcggcg ccggccccgc aggtgctggc ggaaggagag gggcggcgtc agcggccgac
     4801 ggaggcgagg taggcgtcga gcacggccac ctgatcggga ttggcctcgc cccgccattc
     4861 ctcgaccatg gtgaggccga tctcgcgcat cttgtccatc acgtcctcag gcgcctcggt
     4921 gacggtcatg ccgtggtcga tgagcacctg ctccctctcc cggctcgcct ccttgctcag
     4981 ctcgaagccg cgctcggagg ccttcttccc ggctgccagc acggcctgct gctggtcctc
     5041 cgtgagggca tcgaaagcgt cgccattgac gatgatggcg ttcttggtgt gcaggctgcc
     5101 ggtgtaggtg aagtggtcga catagtccca ggcctgcacg tcggtgccgg tctgggcact
     5161 ggtccagagc gcctcgatca acccggtcga gaaggcctgc ggcacctcgg cgaagggcag
     5221 gatggtggcg ttcatgtcga gcatctcgcc catctgctgg gtctgcggcg agtagatgcg
     5281 cagcgactga cccttgaggt cggcgagcga ggtgaccggc tcggcggtgt agaagccctg
     5341 tcccggccac gagacatagg tgatgatgcg catgccgttc tcttcgaaca gcttcgagag
     5401 gtagacgtcc tgcgcctcca tcagcttcca ggcctcgtca taggaggtga cgagattggg
     5461 gatgctgtcg aggccgtaca tgatgtcctc gttgccgtag accgagaggc ggatctcgcc
     5521 ggcctggacc tggcccgact ggaccgcgcg cttgatggcg tcgtgcttga tgagcgaatc
     5581 gttggagcgc aggtcgatga ccagctcgcc gcccgtggcc tccttcacgt cctcgatgaa
     5641 cagccggatg ttcttcgtga agaagctgtc ctcgggatag cccgacgcca tgatccagct
     5701 ggtctcggcc agcgccggcg tcgcgaatgc catcagcgcc gccgccgcga tcgtcgtcgt
     5761 gctcctgctc atcggtttcc tcccgttgcc tgtggcgttc acgatgtcgg tcgggctccc
     5821 gcctgtcaat tccgcggctc gtagtcggcc cgctgcgccg cctcgatata gtcgtgcgcc
     5881 agatcggcct cgatcgaggc cggggcgcgt tcccgcgggt cgccgtgacc gccgccgccg
     5941 ggcatgccga gcacgagacg gcggccgacg gggacgctct ggcggccctt gggcgccagc
     6001 ttcgtgccgt cgtcgaggcc gacggtgccg gcgcgaccgg gcctgccgcc cttgcggcca
     6061 cgggccgggt ggttggtgcg gtcgaacatg gcgttgaagg cgatctcgta gccgttggcc
     6121 gcggcgatct cgatcacctg cccgaggccg ccccggtggc ggccggcacc gcccgagccg
     6181 tcgcgcagct ccttgcgcca gacgacgagc gggccgacct gctcggtcgc ctcggccggc
     6241 atggtcatga cgccggaggg aaaggccgtg gcgttgaggc cgtcgagggt cggcctcgcc
     6301 ccggtgccgc cgctgttgaa gagcagcatt tccgctggcc tgccggtcgt gttcgacggc
     6361 agcgggcgga cggagaactg gatgttccac aaggcgcccg aaccctcggc cggcacccgg
     6421 tccggcaggg caccggccag cgcacccatg acgaggtccg gaacgaagtg gccgagcacg
     6481 tgacggacgg agaccggggc cggcggctcg gcattgagga tgcagcccgg tggtgcggag
     6541 atctcgaagg gggcgagcga ggcggcgttg ttggggatct cgggggcgac gacacatttc
     6601 aggccgtagc aggcataggc cttgcaatag accaggggca cgttgatgcc ccagcggctg
     6661 acctccgacg tgccggtgaa gtcggccttg atgcggtcct cctcgatctc gagggtgacg
     6721 gcgaggtgca cgggctcgtc gtagccgtcg agcgtcatgg cattggtgtg gcggcccctc
     6781 ggcagctcgg cgatcagcgc ccgggtggcc cggtcgctgc gttcgaggat gaagctgccg
     6841 atgccgtcga gatcgtcgaa gccgaactcg tccatcatgg cgaagaggcg cttcatgccg
     6901 gtctcgttgc aggcggcgag cgcgtagaga tcgcccacca ccttgtccgc ctcgcggaca
     6961 ttggcgcgga cgatcaggat caggtcgcga ttgaccgcac cttcacggaa cagcttcatg
     7021 atcgggatga agaggccctc ttcgtggacg tcgcggccgt ccgggccgaa gccgcggccg
     7081 ccgacatcga cgacatgggc ggtgcaggcg aagtagccga tgagcgcacg tttacggaag
     7141 acgggggtga cgacggtgac gtcgtgcagg tgcccggtgc ccttccaggg atcgttggtg
     7201 atgtagacgt cgttctcgag gatgttgccc gggccgatgt cggtgatgaa atgcccgacc
     7261 gattcggcca tcgcattgac gtggccgggg gtgcccgtca ccgcctgggc gagcatccgg
     7321 ccggccacgt cgaagacgcc ggcggaaagg tcgccggcct cgcgcacgct ggtcgagaag
     7381 gcggtgcgga ccagggtcag cgcctgctcc tcgaccaccg agatcaggcg gttccacatg
     7441 acctgcatgt ggatgtcgtc gaggcccttg gccatcagcc ttctcccttg cggcggagga
     7501 cgaggaggca gccgtcggcc tggaccacgg ccaaggagcc ggtcgtgaga atggtcgccg
     7561 tctcgtcctc gaccacgacc gccgggccgg cgagctcggc accggcctcc aatgcgtcgc
     7621 gggcgaagac ggcggcatcg acgaagcggc cctcgccggg ctcgaagagc cgccgcgagc
     7681 cgcgcggccg ggcgggacgg ccctccctgg ccagctcgag cgtcggggtg ggcggcaggc
     7741 gggtggccac cttcagggcg aagctcacga tctcgatcat tgccccctcg accgggcggc
     7801 cgaagaagcg ggtataggct tcggcgaagg cccgggcgac cttccgctcg tcgccggcgg
     7861 cgtagccgcc aggctcgatc gcgacgggaa tctcccagcc ctggccggca tagcgcatgt
     7921 aggcggtgca ttccgtcagc tgctccgcat cgccgccgcc ctgggcgacg aaggcggcgg
     7981 cctcggcgcg catcgcgtcg agcagcgcgt tcaccgcatc ggcgtcgaaa tcgtgcaagg
     8041 gggtcagccg gctgcgcacc gattcgtagc cgaaaggggc gcgcaggaag ccgatggccg
     8101 agccgacgcc ggcgccgggc ggcaccagga agcggtcgat gcccatcttc tcgcacaggc
     8161 gtgcggcgtg cagcgggccg gcgccgccga agcagatcat ggtaaagtcg gcgagctcct
     8221 tgccgttctc gacggcatgc acgcggccgg cattggccat gttctcgtcg accatctcgg
     8281 cgatgccgta ggaagcggcc tcgccgtcga gaccgagcgg ggcgccgacg gcacgctcga
     8341 cggcaccgtt cgcggcggcg ctatccagcg tcatgctgcc gccggcgaaa tcgtgcgggt
     8401 cgatgcggcc gaggagcagg tcggcgtcgg tgacggtggg ctcgctgccg ccccgaccgt
     8461 agcaggcggg gccgggctcg gagccggcgc tcctggggcc cacgcggatg cggcccatca
     8521 ggtcgaccga ggcgatcgag ccgccgccgg caccgatctc gaccatctcg accaccggga
     8581 tcgagatcgg catgccgctg cctttcttga agcgccatgt acgggcaacc tcgaaggtcc
     8641 gcgcggtctt cggcgtctgc tgctcgatca ggcagatctt cgccgtggtg ccgcccatgt
     8701 cgtaggagag cacccggtcc aggccatagc gccgggcgat gtcggcggcg aagaccgcac
     8761 cgccggcggg acccgattcg accaggcgga cgggaaactc ggcggcgctc tcgacgccga
     8821 tgatgccgcc gccggaatgc atcaaaaaga gcgggcagcc gacgccgatg gcgccgagct
     8881 cgtcgaccag ccgcctgagg taggacttga tcagcggctt gacgtaggcg ttggcgcaga
     8941 ccgtgttgaa gcgctcgtac tcgcgcatct gcggcgaaac ctcgctcgag atcgagatct
     9001 ccagacccgg cagccgcttc gccagtgcct cgcgcatccg ctgctcgtgc gcgtcgttca
     9061 tgtaggaatg gatgaagccg atggcgacgc tcgcgtagcc acgctcctcg atggtggcgc
     9121 aaagggcttc gatctcgctc tgctccggtg ccaccagcac gcggccctgc gcgtcgatcc
     9181 gctcggccag cgtcagccgg tcgatccgtg gcacgagcgc cggcgggagc tggatgttca
     9241 aatcgtactg ctcgaagcgg ctctcggtcc gcatctccag gacgtcgcga aagccgcgcg
     9301 tggtgacgaa gccggccctg gccccgcgcc gctcgatcag cgcgttggtc gcgagcgtcg
     9361 tgccgtggat cagaagcgcc agctcacccg gcgccacacc cgctgtccgg gcggcggcct
     9421 gcaggccctg gaggacgccg cgctcgggcc gctctcggtc cgtgagcacc ttggcgctgt
     9481 gaacgacgcc gttcgcctcg atggcgatgt cggtgaaggt gccgccgatg tcgatgccgg
     9541 cacgcgcacg cgccgctgct gccgtcgttt ccaccaagcc ctgcttccga tcgaggtcat
     9601 tgcccgaggg cgggcgagat agaaccgaca ccgcccacca cgctctccga cgatatagaa
     9661 taggatcggc ggcgtcgaca cggggaaggc tcctcatcag ccggagcgca gccgcggcaa
     9721 cttcccatca tgccgccggg aagctacgaa tcgcgcatgt tgcggatcac acgcgaggac
     9781 atcgacgacg ccgtcggcga cgtattcgcc gatcgcggcg aggcgtattt cgcccgcggc
     9841 aaggtgcagg gcctgcggta cgaagagggt atcggacggc tcaccggccg ggtgcagggc
     9901 tcgacgcgga cgccctatcg cgtggacatc cacctcgagc cgcggggcca aggcggcctg
     9961 gaggtcgagg gggtatgcag ttgcccggtc gggatcaact gcaagcatgt ggcagcactg
    10021 ctgttcgccg cacaggcccg attgccggaa cagagggcgg tcgaggtggt ggccgcgccg
    10081 tcggctggcg ctccccggcc cgaggagggg cccgaccccc ggccggagga cgggctcgac
    10141 gagcccgctc gccgctggct ggcggcgctg cgggcggcgc aggcggacga cgcgaacagc
    10201 tttgcggaca gcgtccagaa ccgggtgatc tacgtcctcc gggtcgagcc ggggcggacc
    10261 tggatcgagc cggtcaaggt gcggctgacc aaggctggcg gctatgccat cgagcggcgc
    10321 gactacgcct atcaactgta cagcagcgcg ccccggccga aattcatcac gcccgccgat
    10381 cacaacatcc tgcagcgcat ccacggcctc ttcgcctacg accgaagtgg cctggagcag
    10441 cgtctcgaga gcggtgccct gacggcgcgg cagatcaccg gcctgatcct ggacatcctc
    10501 gccaccggtc gctgctgctg ggccggcccc gatcccgcga ggctgctggc cccgggcgcg
    10561 ccgcggcccg ggaccgcgcg atggaccgcc gacgaacggg gcgaccagag cctctgcttc
    10621 gtcgcggatg acggcgggcc cgcgttcgat ggcgtgatcc cgatcgatcc gccctggtac
    10681 gtcgacctcg aggccggggt ttgcgcgccg atcgagaccg gtctggacga gcggctggcc
    10741 ggcacgctgg cccgagcccc gcgcctcgca cccgcggcga ccgtgcgcct gcgcgaggcc
    10801 atggcggcgg acctgccggc cggcctctcg ataccgctgc ccgccgcctt caagcgggtg
    10861 cgggccgagg cggtgccgcc ggtgccggtg ctgcgtctgg gctgtgtcgg actgaagctc
    10921 gagatgcccg gacgccggca ctatcactgg tatgcgcgcc cgacggtcgc ggtcgacctg
    10981 ccggtctttc ggctggcctt cgactatggc ggggtgatcg tcgagccgtc gagcaagggc
    11041 gagccggagc ggcgcgaggg ccagcgcatc gtcgtcacgc cacgcgacgc cagggccgag
    11101 gcacgggcgc agagccggct ggaccgcctc ggcctcgtgc ggctgggcga ccgccgctcg
    11161 cccttgaagg gggtgaccgc cgagcacgcc gaggcgcgcg ttcccgaatt cgacgacgtg
    11221 gcggtggcgg aactgctcga ggagtccgtg ctcgaccaga gtgcctatct cgccatcgcc
    11281 ggagacggcc tgcccaggct ccagaccgaa ggctggcgca tcgaggagga cgagagctgg
    11341 cccttccggc tggtcgccga gccggtggaa tggcaggcgg agatcgccga gggggagggc
    11401 tggggctggt tcgacctcag gctcggcatc gacatcggcg gcgagcgctt cgatctcctg
    11461 ccgttcctga gcgggctgat cgcccaactg ccgcgcagca ccgatggcgg gccgcccgac
    11521 gacgagacgc tcgaggcgat cttcaccgac atggtgctct atccgcgcct cgccgatggc
    11581 cgcctcctgc ctttggccgg cgagcgcctg ctcctgccgg tcaaggcgct gttcgccctg
    11641 ctggcgcgcg agccggcgac cgacgccgtg gcccgcgtgc atgtcagcga cctcgacgcg
    11701 ctcgtcgacg aggtggtgct cgcctggcag ggtcgcgagc gcctcgatgc gctgcgggcg
    11761 gcgatcgcga gcctgcgcgc cgccccggag atcgatccgc cggcgggctt cggggccgag
    11821 ctgcgcccct accagcgcgc cgggctggcc tggctgcagg cgctgcgcca ggccggattc
    11881 ggcggcatcc tcgccgacga catgggcctc ggcaagaccg tgcaggcgct ggcgcatctg
    11941 gcgatcgaga aggccagggg acggctgtcg cagccctgcc ttctggtcgc gccgaccagc
    12001 ctgatgggta attggtggcg agaagcggcg cgcttcgcgc cggagttgcg gctggtcttg
    12061 tggcacggcg gcgatcgcac catgcgcgcc gccgatctgg acggggccga tctggtgcta
    12121 accacctacg cgctcacccg gcgcgacgag gcgctgctgc tcggccgccg ctggtcgatg
    12181 ctgatcctcg acgaggcgca ggcggtcaag aacccacgct cggcgacggc ccggatcatc
    12241 ggccggatcg aggcgggcca gcgcctcgcc ctgaccggca cgccgctcga gaacaatctc
    12301 gacgagctct ggtcgctctt tcacatcatt gccccgggcc tgctcggtga cctgaagcgg
    12361 tttcgctcga gcttcagggc gccgatcgag aaggatcggg acgcctccgc caacgcccgc
    12421 ctcgccgccc gcatccgccc gttcatgctg cgccggacca aggaggcggt ggcgagcgag
    12481 ctgccgccga agacggagat cgtcgattgg gtcgagctcg cgggggcgca gcgggatctg
    12541 tacgagaccg tgcgcctcat gatggatgcc aaggtgcgaa gctcggtcga gcggatgggc
    12601 ctccagcgca gccacatcgc cattctcgat gcgttgctga agctccgcca ggtctgctgc
    12661 gacccggcgc tggtcaagat cgacggcggc cgccgggtcc agggctcgat caaacgcgag
    12721 cgtcttctgg atctgctcga gacgctcctg gccgagggcc gccgggtgct gctgttctcg
    12781 cagttcgtcg agatgctgaa gctgatccag ggcgacctcg atgcccgcgg catccatcgc
    12841 tcgctgctca ccggccgcac ccgcgatcgc caggccgaga tcgaccgctt cgagggcggc
    12901 cagacgcggc tcttcctgat cagcctcaag gccggcggca ccgggctcaa cctcacctcg
    12961 gccgacaccg tcatcatcta cgatccctgg tggaacccgg cggtcgaatc ccaggccatg
    13021 gaccgcgccc accgcatcgg ccagaccaag ccggtcttcg tctacaagct ggtcgcggag
    13081 ggcacggtcg aggcggtcat ccaggagatg caggcgcgca agcgcgcttt ggcccagggc
    13141 gtctacggcg acgacgccga aaggccgctc gatctggacg aggaggacct gatgcagctt
    13201 ctggcgccga tcgggggcga caagcgagcg agccccgggg gttcccgccc gtagctctcg
    13261 gcacaaaaaa agccgccggc gcgctcgaat gagcggccgg cgcagtcggg gaggcacggg
    13321 gagaaagggg aggctggcgt cagccgcaga cgtcggactt gccgatcgag ccgatctcgg
    13381 agcaggcctt ggtgccgagc ccgatggcgg cgttggtgtt ctggttcacc agcgtcgtgt
    13441 tgttgacgtt gccgccgacc gtcgcgttgt tgaccgtgcc gatggccgag ccggccttcg
    13501 agccgaggcc gatggcggcg ttggtgttct ggttcaccag cgtcgtgttg ttcaggttgc
    13561 cgccgacgtc ggagccggtg accgcaccga tctgcgtgct ggccttcgag ccgaggccaa
    13621 tggcggcgtt ggtgttctgg ttgaccagcg tcgtgttgtt cagattgccg ccgacggtcg
    13681 tgtcctggat gctgccgacc cgggtattgg ctttcgcacc gaggccgatc gcggcgttgg
    13741 tgttctggtt gagcagggtc gtgttgttgg cgttgccgcc gatctgcacg ccgccctgct
    13801 gggccatggc gtgaggtgaa agggcgctcg ccagcagcga cagggtcagg gcggcggcgg
    13861 tgagcttcag ggtctgcatc tcgggtctcc atcgggtggg ggtgttcgat gcgacggaga
    13921 atgccgcgtt cctcgccaac cgtaaggctg gggtttttcc ggggtcgggc ttcgacgccc
    13981 gcctgcgcca tgcgaacatc tgtgcgaaaa cgccctaggc agccgtgccg gaagacgcca
    14041 tcatcccggg cgtcgatcgc gggtgacagg ggaaggaacc gaccatgcgg gctctcgttg
    14101 gggcattgat gctggcaggc gccggcattc tgggattggc accggcagat catgcgacag
    14161 cacagcagac gctgcgcttc gcgcatttct cgtcgaccga ggaccccaac cacgtggcgg
    14221 cgctccgctt caaggagctg ctcgaggccg agacggacgg ccgctacacc gtcgatatct
    14281 acgagaacag ccagctcggc ggcgaggtcg acgtggtcga gggcatccag ctcggcacgg
    14341 tcgacgtctc gccgccctcg gcggcggtgc tggccaacgt cgtcaccgag atgaacatcc
    14401 tcaacatgcc cttcctcttc cgcgactggc cgcattacga ggcggtgctc gagggcgagt
    14461 tcttcgatgc gatcgtcgag gtcgcggccg ccgacgggat ccgcatcctc ggcttcatga
    14521 ccaccggccc gcgccacatc atgaccaagt tccccgtcga gagcatggcc gacctcgcgg
    14581 gcaagaagat ccgcaccgtg cagaacccgg tgcacgtcgc caccttcacc gccttcggcg
    14641 ccaacgccac ggcgatcgcc tatcccgagg tctacggcgc cctgcagacc ggcgtggtcg
    14701 acggcggcga cgccgccaac accaactact acacggccaa gttctacgag gtcgccccct
    14761 actgggcgca ggtgagctgg ctcttctact ccaacccgct ggtcatgtcg gaagacgcct
    14821 tccaggcgct gagcccggag gaccaggaga tcttccagcg cgtcggccgc caggtcgggc
    14881 gcgagcagat cgccaactgg aaggcgagcg acgccggcct gctcgacgag ctgaagaagg
    14941 cgggtgtgac cgtcaccacg ccgccgcgcg agcccttcca ggaggccgtg ggcgaggtct
    15001 acgacacctt cctcgccagc gacttcgaga aggaatggct ggggcgcatc caggacaccg
    15061 agtgacccga gcgggcggcg gagggcggcc atgccggccc ggctcctgac cgccctcgcc
    15121 ctcgtccgcc gcgtcttccg cctcgccacc atcctctgcc tcgtggtgat gacgctctcg
    15181 atcaccgtgc aggtggtcgg ccgctacctc ctctccttcc cgatcgccga cgcggtcgag
    15241 atctcgacct tcgcgcaagt ctggctggtg ctgctgggtg cgggcatcgc ctgccggcac
    15301 gacgcgctct tcaccgtcga cctcttcggc cagcgcctgc cgcccgtgct gcaccggctg
    15361 cggggctggc tgatcctggc ctgcggctcg gtcttcctcg gcctcctcct ctggggcagc
    15421 ttctcgctcc tcggcctcgg cctcaggcag cgcgcaccca ccctgcagat cccgatgatc
    15481 tggatctacg ccgccctgcc gatcggcttt ctctacttct ggatcgagct cgccgtccgc
    15541 tcgctcgacc tcgagccggc cctttcggcc gaggcccgga acggcgagac ccggtgatca
    15601 tcgtcgtcct cgccttcctc gtcctcatgg ccctgggcgt gccgatcgtc ctcgccctcg
    15661 gcagcccggt cgccctctac atcctgatca gcggcgacgt gcccatcgtc gtgatcgcgc
    15721 accgcttcta cagcggcctc aacaacttca ccctgctcgc catcccgttc ttcgtcctcg
    15781 ccggcctcct gatggaagcc ggcggcctgt cgcggcgcat cgtcgacttc gccagctcgc
    15841 tggtcggctg gatccagggc gggctcctca tggtcgccgt cgtcgcctcg acggcgctgg
    15901 ccgccatgtc gggctcgggc agcgccgacg ccgccgccgt ctccgccgtg ctgcagcccg
    15961 agctgcgccg ccggcactac gagatcgact tcgccgccgc cctgatcgcc gcctcggcga
    16021 cgctggccca gatcatcccg ccgagcctga tgctggtcat catcgccctc cagaacaacc
    16081 tctcggtcgg cgccctcttc ctcggcggcg tgctgccggg cc
//
LOCUS       DAOTFI010000110        15975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4411_length_15975_cov_6.829397, whole genome shotgun sequence.
ACCESSION   DAOTFI010000110 DAOTFI010000000
VERSION     DAOTFI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4411_length_15975_cov_6.829397"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..342)
                     /locus_tag="P5558_12470"
     CDS             complement(97..342)
                     /locus_tag="P5558_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRY25171.1"
                     /translation="MAELARLEAMPDERIDTDDMPELTAAQLADMERGRFYRPVKQQV
                     TARLDADVLAWLKAGGQGYQSRMNAILRRAMLADREA"
     gene            complement(383..685)
                     /locus_tag="P5558_12475"
     CDS             complement(383..685)
                     /locus_tag="P5558_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRY25172.1"
                     /translation="MMEIRFTWDTAKAGSNRRKHGVDVEDALGVFRDPLHLSRLDRVE
                     DGEQRWQTIGRAHGVTVLLVAHTMTEEGSGTGEVIRIISARKATRSERKRYEEQDR"
     gene            complement(837..1328)
                     /locus_tag="P5558_12480"
     CDS             complement(837..1328)
                     /locus_tag="P5558_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRY25173.1"
                     /translation="MAGRAARAPQGGGDGGGAELKITVLALGRNRDADLERLIQGYVA
                     RCPWPVRIDELVPRKGESEAARLGRALPDGAAVTALDERGELATSEDLAARIGGWRDE
                     GRDLAFLIGGADGLDDATRARADWRLALGRLTWPHLMVRLLLVEQLYRASTILAGHPY
                     HRS"
     gene            complement(1268..1564)
                     /gene="rsfS"
                     /locus_tag="P5558_12485"
     CDS             complement(1268..1564)
                     /gene="rsfS"
                     /locus_tag="P5558_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRY25174.1"
                     /translation="MDLAGKTTLADHMVVATGTSQRHLASMAEKLIHRLKAAGLDGIQ
                     AEGLGDSSWVLIDAGDVIVHLFRAETRSFYDIERLWQVVPRERLKAVATAVEPN"
     gene            complement(1651..2349)
                     /locus_tag="P5558_12490"
     CDS             complement(1651..2349)
                     /locus_tag="P5558_12490"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRY25175.1"
                     /translation="MTSRIPGLDEAPPLRRRRGPRLFRRVPEAPPLPRKPTRAIGLLG
                     GSFNPAHEGHLYVSREALKRLGLDQVWWLVSPQNPLKPRAGMAPFEDRLAYARALVDD
                     PRIRVSDLEARLDVTYTADTLARLARLPGQRFIWLIGADNLAQLPLWRHWRRIFALAP
                     IAVFDRAPHSSSAAAGRAASVFGHARLPEREALHLKDRPTPAWVYLHLPPHPASSTAI
                     RAGRQDAAIRETAP"
     gene            complement(2368..3645)
                     /locus_tag="P5558_12495"
     CDS             complement(2368..3645)
                     /locus_tag="P5558_12495"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRY25176.1"
                     /translation="MATALQDTLASEIEAMGRKARAAATALATAPRVLKDEALRAAAA
                     TLRRQESAVLAANERDVAAGRARQLTPALLDRLALDSKRIAGIARSLEAIAELGDPVG
                     QEIARWQRPNGLDIARVRTPLGVIGVIYESRPNVTADAGGLCLKAGNAVILRGGSESS
                     HSSAAIHACLVEGLATAGLPVDAIQLVATTDRRAVGHLLALSRYVDVIVPRGGRSLIE
                     RVQAEARMPVFAHLDGICHVYLHSSADPAMARAIALNAKMRRTGICGAMETLLCDRSV
                     AGALLPPILDDLARAGCELVGDAEVMALDPRVGAATEEDWRTEYLDAILSVKLVDGLE
                     EAIGHIEAHGSHHTDSIVAGDQGAAATFLARVDSAIVMHNASTQFADGGEFGMGAEIG
                     ISTGRMHARGPVGVEQLTSFKYQVRGNGQTRPG"
     gene            complement(3714..4559)
                     /locus_tag="P5558_12500"
     CDS             complement(3714..4559)
                     /locus_tag="P5558_12500"
                     /inference="COORDINATES: protein motif:HMM:NF023354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HRY25177.1"
                     /translation="MSPELGRLLLDLLGRPYRLAMGLHALDLQDWLVVTADHAAEVAE
                     KRRLLDAGADIHRLLPEGAAAAAETAALVEAHLATHAPAVRVDAGIADPLIRAGLAVQ
                     EDLCLLEGSPEGYRLVGAFVAFPSRWWLAEKIGRPMPAIHAPVPGLEAAIGGPVRLFF
                     ERLQADRPVWRVNWSLTDDPLLHQPSSAFRRTATEVTAAEAGRQLWLRVERQTLRRLP
                     RTGLVLFTILTFVEPLASLAQDRRLALLLADRLDELPAAMLTYKNLATRLPAITSYLR
                     AAAAA"
     gene            complement(4556..5722)
                     /gene="proB"
                     /locus_tag="P5558_12505"
     CDS             complement(4556..5722)
                     /gene="proB"
                     /locus_tag="P5558_12505"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRY25178.1"
                     /translation="MQPAALLARRRVTIKIGSALLVDGEGRIRRAWLDGLAADIGELA
                     RRGSRVIVVTSGAIALGRRALGLPQRALRLEEKQAAAAAGQILLAGAWAESLAGHGLI
                     AAQLLVTLGDTEGRRRYLNARATIETLLKLGAVPVVNENDTVATAEIRYGDNDRLSAR
                     VAVMSGAETLVLLSDVDGLYTADPGRDPAARHIPEVGAITAEIEAMATGSGSAVGTGG
                     MASKLVAATIATQSGCAVLLTSGKPDRPLEALRSGARCTVFAARSTPRRARKHWLAAT
                     LKPAGRLTVDDGALAALRRGSSLLPAGVAAVEGRFERGDAVLVQNLQGTVVAKGLVAY
                     DAADAERLRGRRTADIEALLGYRGRDEMIHRDDLVLVQAPGEPAGEAAVGTEAP"
     gene            complement(5722..6750)
                     /gene="obgE"
                     /locus_tag="P5558_12510"
     CDS             complement(5722..6750)
                     /gene="obgE"
                     /locus_tag="P5558_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRY25179.1"
                     /translation="MRFLDSAKVFVKSGDGGNGSSAFRREKFIEFGGPSGGDGGRGGD
                     VVAIADPDLNTLIDYRYRQHFKAGRGEGGAGQERTGASGKDVELRLPVGTQILAEDGL
                     TLIADLKEPGERIVLAKGGDGGFGNVRFKTSTNRAPRRADPGWPGEERWLWLKLKLLA
                     DAGLVGLPNAGKSTFLAAVSRARPKIADYPFTTLEPKLGTIVMDDRSFVIADIPGLIE
                     GASEGVGLGDRFLGHIERCATLIHLVDGTAEHPDEAWRTVRAELEAYGHGLAEKPEIL
                     CLNKIDALTPEVIEERKHLLEAASGEPVRCISGVAKEGLEAVLREVLDHVGARRAAEA
                     EAGAADQS"
     gene            complement(6856..7125)
                     /gene="rpmA"
                     /locus_tag="P5558_12515"
     CDS             complement(6856..7125)
                     /gene="rpmA"
                     /locus_tag="P5558_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRY25180.1"
                     /translation="MAHKKAGGSSRNGRDTAGKRLGVKKFGGEHVIPGNIIIRQRGTK
                     YHPGKGVGMGRDHTLFALVEGHVTFRKSGDRQFVQIAPPVAQVAE"
     gene            complement(7154..7468)
                     /gene="rplU"
                     /locus_tag="P5558_12520"
     CDS             complement(7154..7468)
                     /gene="rplU"
                     /locus_tag="P5558_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRY25181.1"
                     /translation="MYAVIKTGGKQYRVAKDDYLRIEKLDAEVGSTVEFDQVLLVGGD
                     GADPKVGAPLLDGAKVTAEVVEQTRGPKIVIFKKRRRKNFRRRTGHRQYLTVVKIADI
                     AA"
     gene            complement(7587..8477)
                     /locus_tag="P5558_12525"
     CDS             complement(7587..8477)
                     /locus_tag="P5558_12525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRY25182.1"
                     /translation="MDTLETLRKRIATTEDLQSIVGTMKALSAVSIRQYEEAVAALRD
                     YSRTIELGLQVVLQRNGPAMAARATVPAPVLAIVFGSDHGLCGRFNHEIAHFAQRALA
                     DAPAGTRYLVAGARPAAVLEAMGETVEQDFLLPGAVGGLSGTVHRILGAIDARRAQEP
                     ATRARLFYNRRTTGGAAVPTSLPLLPLDPGWLAGLAQRRWAGRSLPSFTMSAEALLAA
                     LVRQHLFIGLFRAAAESAASEHATRLAAMQAAERNIEERLGEMNADYRRKRQDSITEE
                     LLDLVSGFEALKPAERLGQD"
     gene            complement(8495..10030)
                     /locus_tag="P5558_12530"
     CDS             complement(8495..10030)
                     /locus_tag="P5558_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HRY25183.1"
                     /translation="MTDRADTSELGAVTDATFDPLDRAVQGYRARPQLSEVGTVVQVA
                     SGIARVTGLQEVRDSELIALPNQLLGIATNLAMDGIGIILLGQGGTLRTGDPATRTHR
                     VVDMPVGPALLGRVVDATGRPLDRDEPIAAEARWPIERPAPPIMARAPVAEPLQTGIK
                     AVDAAIPIGRGQRELILGDRQTGKTTIALDAIINQRRTGMVSIYCAIGQRGAAVARAV
                     AVLKEHDALERCVVVVAAGDDPAGLQFVAPYAATTIAEYFMEQGQDVLIVYDDLTRHA
                     RAYRELSLLLRRPPGREAYPGDIFYIHSRLLERSTHLREELGGGSLTALPIIETQAQN
                     LSAYIPTNLISITDGQIYLSPELFRKGQLPAVDIGKSVSRVGGKAQLPALRAVAGDLR
                     LGYAQFEELETFARFGTRLDEATRQSLARGQRVRAILRQAEHDPLTVPEQLVVVLAAT
                     TGVLDDVPVERVAEAEAALRRAVTADMPELGPRLASGEKLGDEERERLLALARSASTP
                     FRA"
     gene            complement(10027..10776)
                     /locus_tag="P5558_12535"
     CDS             complement(10027..10776)
                     /locus_tag="P5558_12535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRY25184.1"
                     /translation="MQIDWLTVAAQIVNFLVLVWLLKRFLYGPITAAMARREARIAER
                     LSLAAEKQAAADREARSFRDERAALEQARERLLREAHEAADKERKALEQEARSAVEAR
                     KQEWLQQLATERETVLRNLRHRSTEAFYTLARRALDDLADTGLEDQVALGFARQLAGL
                     DDAAAAAITAAADGRVRVRSRFELGAEARRQIDRAVHERLDPGIEVAYTVDAGLACGV
                     VLEAGSQRLSWSLASYLDGFEAAVGKELAGA"
     gene            complement(10780..11058)
                     /locus_tag="P5558_12540"
     CDS             complement(10780..11058)
                     /locus_tag="P5558_12540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HRY25185.1"
                     /translation="MDSIALIAAVSIFTAGITIAIGSIGPALGEGRAAAQALSAIAQQ
                     PDEANRITRTLFVSLAMIESTAIYCFVVAMILIFANPFWNYVISQGAG"
     gene            complement(11072..11785)
                     /locus_tag="P5558_12545"
     CDS             complement(11072..11785)
                     /locus_tag="P5558_12545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRY25186.1"
                     /translation="MEISPDQIVYFHLGPVAINATILFTWVVMLLLTAGSWLVTRRLR
                     DTETMSRWQNLLEVLVEGMGDQIREVSQTEPGRYLPFVGTLFVFIASSNILGIVPGFR
                     PPTGSLSTTTALAICVLVAVPLYAIVENGVAAYLRQYLQPTFFMLPFNIIGELSRTLA
                     LAVRLYGNVMSGTIIIAILIGVVPFFFPIVMELLGLLTGIIQAYIFAILAMVYIASAA
                     QVEAAGAAASDRHEPNQPS"
     gene            complement(11789..12106)
                     /locus_tag="P5558_12550"
     CDS             complement(11789..12106)
                     /locus_tag="P5558_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRY25187.1"
                     /translation="MTEPSLAMAWDPAALALAFGFGLLAGAAYLASLWVSVRRLGSSA
                     APLPRLLIGAALRIALLVGAIVVVMGGEWQLMLAALAGFIVMRIAVTTWARTTLSTSR
                     GST"
     gene            complement(12099..12404)
                     /locus_tag="P5558_12555"
     CDS             complement(12099..12404)
                     /locus_tag="P5558_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRY25188.1"
                     /translation="MAGPEGPSRHDETVGEAIGRKAERKLEAQGARHRGVWFGLGMFG
                     LVGWSVAVPTLLGIALGIWLDGAVPSRFSWTLSLLFIGAVLGSINAWYWVERENRRD"
     gene            complement(12404..12826)
                     /locus_tag="P5558_12560"
     CDS             complement(12404..12826)
                     /locus_tag="P5558_12560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRY25189.1"
                     /translation="MPPDQRDGAGLMRLEVLLPTEVLVDRPVTRVVAEAENGSFGLLP
                     RHVDFAAALRPGILIYTDAEGRERYLGIDEGILVKCAGEVLVSTQSAVVGDDLESLRA
                     TVQRRYVELDEGQKVARSTLARLELGVVRRFLELQEIG"
     gene            complement(12816..14246)
                     /gene="atpD"
                     /locus_tag="P5558_12565"
     CDS             complement(12816..14246)
                     /gene="atpD"
                     /locus_tag="P5558_12565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRY25190.1"
                     /translation="MRDGHASTREGRPAEGRIVAIQGTVVDVRFEHDLPMIDHMLRAG
                     PDQDAIIEVMGLLDPRTVRGLALRAPHGLALGMRVSDTGTPIQVPVGRAVLGRMLDMF
                     GEVIDGKPPLEGVERRSIHARPVPLRQRVVRSEIFETGIKAIDLLCPLERGGKAGLFG
                     GAGVGKTVLITEMIHNMVGKYEGVSLFCGIGERCREAEDLYREMQTAGVLDKTVMIFG
                     QMDESPGVRFRVGHSAMTIAEHFRDDARQDVLVLIDNIFRFVQAGSEVSGLLGRMPSR
                     VGYQPTLGTELAALEERISSTASGAITSIQAVYVPADDFTDPAATHTFAHLSASIALS
                     RKRASQGLYPAIDPLQSNAKMLTPGVVGQRHYRIAGEVRQTLAEYEELKDIIAMLGLE
                     ELAEKDRQIVSRARRLERFLTQPFFSTEAFTGKPGRLVALDDTLDGCERILNDAFADQ
                     PESAFYMIGALDDLEQRLARGQPDAA"
     gene            complement(14313..>15975)
                     /locus_tag="P5558_12570"
     CDS             complement(14313..>15975)
                     /locus_tag="P5558_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRY25191.1"
                     /translation="DDEPPCLRRDGEDAAEDPALRKALEIGALCTSAELAENDRGGKG
                     DPMELALLAGAAAAGIDREALLERQPELRREEFEPATRMMATFHQAEGGVRVTVKGAP
                     EAVIEACTRVRSGEEAEALDEAGRARWRERNEALARSGLRALALAEKSATSADDQPYQ
                     DLELIGLVGLLDPPRRDVRDAIRACREAGIRVVMVTGDQPATARAIAEAVGLVDAGDD
                     VPIVHGGDLGQVEAMSEAERRRVLEASILARVDPKQKLDLIRIHQEDGARVAMTGDGV
                     NDAPALKKADIGIAMGQRGTQVAREAAHMVLRDDSFATIVLAIAQGRVIFDNIRRFIV
                     YMLSGNAGEIFAVAVLALLNAPLPLLPLQILYINLISDVFPALALGVGRGESPLDRPA
                     RDPDEPIVARRHWLAIGGYGVLIGSSVMAVFAYALLVAGMATEQAVTISFLTFALGRL
                     WHVFNMREPGSDLLRNTVTTNPWVWGALGVGIALLAAALYVPLLADVLETVDPGATGW
                     LLIAIGSLVPLVIGQILKSPAVRAALPSRLLPAAAARQAPRSRSG"
BASE COUNT         2296 a         5589 c         5758 g         2332 t
ORIGIN      
        1 aaagtgtcac ccatgtctcc gtacagctgt taatgatgtc tccggtccat acaggaggcc
       61 agcctccccg tccttcttcg ctgcgatccc gacgtctcag gcttcgcggt ctgccagcat
      121 ggcgcggcgg aggatggcgt tcatgcgact ttggtagcct tggccgcctg ccttcagcca
      181 ggccaggaca tcggcgtcca gacgtgccgt gacctgctgc ttgacgggcc ggtagaatcg
      241 gccacgctcc atgtcggcga gttgtgccgc ggtcagctcg ggcatgtcat cggtgtcgat
      301 acgctcgtcc ggcatggcct cgagccgggc gagctcggcc aaccgctcag gggtcgatcg
      361 cggtgcgctc tctgctgtgt agctaacggt cctgttcctc ataacgcttc ctttccgatc
      421 gcgtggcctt tcgggcggaa atgatgcgga tcacctcccc ggtccccgag ccttcctcgg
      481 tcatcgtgtg ggcgaccagc aggaccgtca cgccgtgcgc ccggccgatg gtctgccacc
      541 gttgctcgcc gtcctcgacc cggtccaggc gggagagatg caatggatcg cgaaaaaccc
      601 ccagcgcatc ctcgacgtcg acgccgtgct tgcgcctgtt cgatccggcc ttggcggtgt
      661 cccaagtgaa ccgaatctcc atcatgccgg aaaagtaatt acatttttgt cgttaatcaa
      721 gcccgctcag tcgggccggg ggcgcacccg cgtcgcccac ccccgccacc tcaaaatccg
      781 gtggggccga tggggaggat catcctcccc atcatcttcc tgccagactg gctggctcag
      841 cttcggtgat acggatgccc cgccagaatg gtgctggccc ggtagagctg ctcgacgagc
      901 agcagccgca ccatgagatg tggccaggtg aggcggccga gggcgaggcg ccagtcggcc
      961 cgggcacggg tcgcgtcgtc gaggccgtcg gcgccgccga tgaggaaggc gaggtcgcgg
     1021 ccctcgtcgc gccagccgcc gattcgggcg gccaggtcct cgctcgtggc gagctcgccc
     1081 cgctcgtcga gggcggtgac ggccgcacca tccggcaggg cgcggccgag gcgggccgcc
     1141 tcgctctcgc ccttgcgcgg cacgagctcg tcgatccgga cgggccacgg gcagcgggcg
     1201 acatagccct ggatcaggcg ctcgagatcg gcgtcgcggt tgcggccgag ggcgaggacc
     1261 gtgatcttca gttcggctcc accgccgtcg ccaccgcctt gaggcgctcg cgcggcacga
     1321 cctgccacag gcgctcgatg tcgtagaagg agcgggtctc ggcgcggaag agatggacga
     1381 tcacgtcgcc cgcgtcgatc agcacccacg agctgtcgcc gagcccttcg gcctggatgc
     1441 cgtccagccc ggccgccttc aggcggtgga tgagcttctc ggccatcgac gccaggtggc
     1501 gttgcgacgt gccggtggcc acgaccatgt ggtcggcgag cgtggtcttg ccggcgaggt
     1561 cgatgacgat gggctcgagc gccttgtcgt cgtcgagcga gctcaggacc agaggcagga
     1621 tcgtgtcggc ggcgggctct tcggtgctgg tcagggtgcg gtctcccgga tggcggcgtc
     1681 ctggcggccg gcgcggatcg ccgtgctgga agccgggtgt ggcggcaaat gcagatagac
     1741 ccaggcgggt gtcggtcggt ccttgaggtg cagggcctcc cgctcgggca ggcgggcgtg
     1801 gccgaagacc gaggcggcac ggccggcggc agcactacta gaatggggtg cccggtcgaa
     1861 gaccgcaatc ggcgcaagcg cgaagatgcg ccgccaatgg cgccagagcg gcagctgggc
     1921 gaggttgtcg gccccgatca gccagatgaa gcgctggccg ggcagtcgcg ccagccgggc
     1981 gagcgtgtcg gccgtgtagg tgacgtcgag ccgtgcctcg aggtcgctga cgcgaatgcg
     2041 cgggtcatcg acgagcgccc gggcataggc caggcgatcc tcgaagggcg ccatgccggc
     2101 ccggggcttc aggggattct gcggcgagac cagccaccag acctggtcga gcccgaggcg
     2161 cttcagcgcc tcgcggctga catagaggtg gccttcatgg gccgggttga acgagccgcc
     2221 gaggaggccg atcgcgcggg tcggcttgcg gggcaggggc ggcgcctccg gaacgcggcg
     2281 aaagagcctg ggaccgcggc ggcgccgcaa gggcggcgcc tcgtcgagcc ccggtatgcg
     2341 tgatgtcacg ccggcgcctc ggcgctgcta gcccggtcgc gtctggccat tgccgcgcac
     2401 ctggtacttg aagctggtga gctgctcgac gccgaccggc cccctggcat gcatgcggcc
     2461 ggtcgagatg ccgatctccg cccccatgcc gaactcgccg ccatcggcga actgggtcga
     2521 ggcgttgtgc atgacgatgg cactgtcgac ccgtgccagg aaggtcgccg ccgcaccctg
     2581 gtcgccggcg acgatgctgt cggtgtggtg cgagccatgg gcctcgatgt ggccgatcgc
     2641 ctcctccagc ccgtccacca gcttcaccga caggatggcg tccagatact ccgtccgcca
     2701 gtcctcctcc gtcgcggcac cgacgcgcgg gtcgagcgcc atgacctcgg cgtcgccgac
     2761 gagctcgcag ccggcccgcg cgaggtcgtc gaggatcggc ggcagcaggg ccccggccac
     2821 gctcctgtcg cagagcagcg tctccatcgc cccgcagatg ccggtccgcc gcatcttggc
     2881 gttgagggcg atggcccgcg ccattgccgg atcggcggag ctgtgcaggt agacgtggca
     2941 gatgccgtcg agatgggcga agaccggcat ccgcgcctcg gcctgcaccc gctcgatcag
     3001 cgagcgcccg ccgcgcggca cgatgacgtc gacataacgg ctcagcgcga ggagatggcc
     3061 gacggcgcgc cggtcggtgg tggcgacgag ctggatcgcg tcgacgggca ggccggcggt
     3121 ggccagcccc tccaccaggc aggcatggat cgccgccgag gagtggctgc tctccgagcc
     3181 gccgcgcagg atcaccgcat tgccggcctt gaggcagagc ccgcccgcat cggcggtgac
     3241 gttgggccgg ctctcgtaga tcacgccgat cacgccgagc ggcgtgcgca cgcgggcgat
     3301 atcgaggccg ttggggcgct gccagcgggc gatctcctgg cccaccggat cgccgagctc
     3361 ggcgatcgcc tcgaggctcc gggcgatgcc cgcgatgcgc ttgctgtcga gggccagacg
     3421 gtcgagcagc gccggggtga gctgccgtgc ccggccggcg gcgacatccc gctcgttggc
     3481 cgcgagaacg gccgactcct gccggcgcag ggtcgccgcc gccgcccgga gcgcctcgtc
     3541 cttcaggacc cgcggtgccg tggccagcgc cgtggcggcg gcgcgggcct tcctgcccat
     3601 ggcctcgatc tcgctggcca gcgtatcctg gagtgccgtt gccatcgcct tctttcgacc
     3661 tttccttgtc aaaccttgct tagcagcaaa cgggtggtcc gggccagcag ccgtcaggcg
     3721 gcagccgcgg cgcgcagata cgacgtgatc gccggcaggc gcgtggcgag gttcttgtag
     3781 gtgagcatcg ccgctggcag ctcgtccagc cggtccgcga gcagcagggc gaggcggcga
     3841 tcttgggcga ggctcgccag cggctcgacg aaggtcagga tggtgaagag gacgagcccc
     3901 gtcctcggca gccggcgcag cgtctgccgc tcgacgcgca gccagagctg ccggccggcc
     3961 tcggctgccg tgacctcggt ggccgtgcgg cgaaaggcgg aggatggctg atgcagcagc
     4021 gggtcgtcgg tgagcgacca gttcacccgc cagaccgggc ggtccgcctg cagccgctcg
     4081 aagaagagcc ggaccgggcc gccgatcgcc gcctcgaggc ccggcacggg ggcatggatc
     4141 gccggcatcg gccggccgat cttctccgcc agccaccagc gcgagggaaa ggcgacgaag
     4201 gcgccgacca gccggtagcc ttccggcgag ccttcgagga ggcagagatc ctcctgcacg
     4261 gcgagcccgg cccggatgag cgggtcggcg atcccggcat ccacccgcac ggccggcgca
     4321 tgcgtggcca gatgcgcctc gaccagcgcc gccgtctcgg ccgcggccgc cgcgccctcg
     4381 ggcaggagcc gatgaatgtc cgcacctgcg tcgagcagcc ggcgcttctc ggccacctcg
     4441 gcggcatggt cggcggtcac cacgagccaa tcctgcaggt cgagcgcgtg caggcccatg
     4501 gcgaggcggt agggccggcc gagcaaatcc aagagcagcc ggccgagctc ggggctcatg
     4561 gcgcttcggt cccgaccgcc gcctctcctg ccggctcgcc cggtgcctgg accagcacga
     4621 ggtcgtcccg gtggatcatc tcgtcccggc cgcgatagcc gagcagcgcc tcgatgtcgg
     4681 ccgtccttct gccccggagc cgttccgcgt cggcggcgtc gtaggcgacg agccccttgg
     4741 cgacgaccgt gccctggaga ttctggacga gcaccgcgtc gccccgctcg aagcgcccct
     4801 cgacggcggc cacaccggcc ggcagcaggc tcgagccgcg cctgagcgcc gcgagcgccc
     4861 cgtcgtcgac ggtgagccgg cccgccggct tcagcgtcgc cgcgagccag tgcttgcgcg
     4921 cccgccgcgg ggtggagcgg gcggcgaaga ccgtgcagcg cgcccccgag cgcagcgcct
     4981 cgagcggccg gtcgggcttg cccgaggtga gcaggacggc gcagccgctc tgcgtcgcga
     5041 tggtggcggc gaccagcttg ctcgccatgc cgcccgtgcc cacggcactg ccggagccgg
     5101 tggccatggc ctcgatctcg gcggtgatcg ccccgacctc ggggatgtgc cgggccgcgg
     5161 ggtcgcgccc gggatcggcc gtgtagagcc cgtccacgtc ggagagcaga accagcgtct
     5221 cggcgccgga catgacggcg acccgggcgg agagccggtc gttgtcgcca tagcggattt
     5281 cggccgtggc caccgtgtcg ttctcgttga ccaccggcac cgcacccagc ttcagaagcg
     5341 tctcgatggt cgcccgggca ttgaggtagc gccgccggcc ctcggtgtcg ccgagggtga
     5401 cgaggagctg ggcggcgatc aggccatggc ccgccaggct ctcggcccag gcgccggcca
     5461 gcaggatctg gcccgcggcg gccgccgcct gcttctcctc gaggcgaagg gcccgctgcg
     5521 gcaggccgag cgcccggcgg cccagggcaa tggcccccga ggtgacgacg atgacccggc
     5581 tgccccgccg ggcgagctcg ccgatatcgg cggcgaggcc gtcgagccag gcacgccgga
     5641 tccgcccttc gccgtcgacc agcagggccg agccgatctt gatggtgacc cgtcgccggg
     5701 cgagcagggc ggccggctgc atcagctctg gtccgctgcc ccggcctccg cctcggccgc
     5761 gcgccgggcg ccgacatgat ccagcacctc gcgcagcacc gcctccagcc cctccttcgc
     5821 cacgcccgag atgcagcgca cgggctcgcc cgaagccgcc tcgagcaggt gcttgcgctc
     5881 ctcgatcacc tccggggtca gggcgtcgat cttgttgagg cagaggatct cgggcttctc
     5941 cgccagcccg tggccatagg cctcgagctc ggcgcgcacc gtccgccagg cctcgtcggg
     6001 atgctcggcc gtgccgtcga cgaggtggat cagcgtggcg cagcgctcga tatggccgag
     6061 gaagcggtcg cccagcccca ccccctccga ggccccctcg atcaggcccg ggatgtcggc
     6121 gatgacgaag ctccggtcgt ccatgacgat ggtgccgagc ttgggctcga gcgtggtgaa
     6181 ggggtagtcg gcgatcttcg gccgcgcccg gctgacggct gcgagaaacg tcgacttgcc
     6241 ggcgttgggc agcccgacca gcccggcatc ggcgagcagc ttcagcttca gccagagcca
     6301 gcgctcctcg ccgggccagc cggggtcggc gcggcgcggg gcccggttgg tcgaggtctt
     6361 gaagcggaca ttgccgaagc cgccatcgcc gcccttggcc agcacgatgc gctcgccggg
     6421 ctccttcagg tccgcgatca gcgtcaggcc gtcctcggcc aggatctgcg tgccgaccgg
     6481 caggcggagc tcgacatcct tgccgctcgc cccggtgcgc tcctggccgg cgccgccttc
     6541 gccacgcccg gccttgaagt gctgccggta gcggtagtcg atcagcgtgt tgaggtcggg
     6601 atcggcgatg gccaccacgt cgccgccgcg cccgccatcg ccgccgctcg ggccgccgaa
     6661 ctcgatgaac ttctcgcgcc ggaaggccga cgagccattg ccgccgtcgc ccgacttgac
     6721 gaagaccttg gccgaatcga ggaagcgcat gcggtttggc cagcgaaggg aagggggtga
     6781 agaggaaaag ggccaaaaaa cgaaaaaggg aaaggcctgg gcctttcccc cgttcgccgg
     6841 cctcttggcg gacgcctact cggcgacctg ggccactggc ggtgcaatct gcacgaactg
     6901 gcgatcgccg ctcttgcgga aggtcacgtg accctcgacc aaagcgaaaa gcgtgtggtc
     6961 acggcccatg ccgacaccct tgccgggatg gtacttggtg ccgcgctggc ggatgatgat
     7021 gttgccgggg atgacgtgct cgccgccgaa cttcttcacg cccagccgct tgccggcggt
     7081 gtcgcgaccg ttcctcgagc tgccgcccgc tttcttgtgt gccatgggat gctcctcggt
     7141 tgcgctcgcc ggctcaggcg gcgatgtcgg cgatcttgac cacggtcagg tactgccggt
     7201 ggccggtgcg gcggcggaaa ttcttgcgcc gccgcttctt gaagatgacg atcttcgggc
     7261 cccgggtctg ctcgaccacc tcggcggtca ccttggcacc gtcgaggagc ggggcaccca
     7321 ccttcgggtc ggcgccatcg ccgccgacca gcagcacctg gtcgaactcg accgtgctgc
     7381 cgacctcggc gtcgagcttc tcgatccgaa gatagtcatc cttggcgacg cggtactgct
     7441 tgccgccggt cttgatcact gcgtacatcg acttgaaccg atccggactg gaagggggcc
     7501 tggggcgcaa cgaaccgggc cgcgacaaag cagtcccgca ggcctcaccc gagagaaggc
     7561 ggaaataggc ggaacccgga cgcctgtcaa tcctgcccga gcctctccgc gggcttgagc
     7621 gcctcgaagc cggagacgag atcgagcagc tcctcggtga tcgaatcctg gcgcttgcgc
     7681 cggtagtcgg cgttcatctc gccgagccgc tcctcgatgt tccgctcggc cgcctgcatg
     7741 gcggcgaggc gggtcgcatg ctcgctcgcc gccgattccg cggcggcccg aaagaggccg
     7801 atgaagagat gctggcgcac cagggcggcg agcagggctt cggccgacat ggtgaagctg
     7861 ggcaggctcc gcccggccca gcggcgctgc gcgaggccgg cgagccagcc cgggtcgagc
     7921 ggcaggagcg gcaggctggt cggcacggcg gcgccgccgg tcgtgcgccg gttgtagaag
     7981 agccgcgcac gggtcgcggg ctcctgcgcc cggcgggcgt cgatggctcc caggatccga
     8041 tgcaccgtcc cgctcaagcc acccacagca ccgggcagga ggaagtcctg ctcgaccgtc
     8101 tcgcccatgg cctcgaggac cgccgccggc cgcgcaccgg cgacgaggta gcgcgtgccg
     8161 gcaggtgcgt cggcgagcgc gcgttgcgcg aaatgcgcga tctcgtggtt gaagcggccg
     8221 cagagcccgt gatcggagcc gaagacgatg gcgagcacag gggcgggcac cgtcgcccgt
     8281 gctgccatcg ccggaccgtt ccgctgcagc accacctgca ggccgagctc gatggtccgg
     8341 ctgtagtcgc ggagcgccgc cacggcctcc tcgtactggc ggatgctgac cgccgagagc
     8401 gccttcatcg tgccgacgat cgattgcagg tcctcggtcg tggcgatccg cttgcggagg
     8461 gtctcgaggg tgtccattgc ccggcgccgg ccggtcaggc ccggaacggg gtcgaggcgc
     8521 tgcgcgccag ggccagcagg cgctcgcgct cctcgtcccc gagcttctcg ccggatgcca
     8581 gccggggacc gagctccggc atgtcggccg tgacggccct gcgcaacgcc gcctcggcct
     8641 cggcgacgcg ctccaccggc acgtcgtcga gcacgcccgt ggtcgcggcc agcacgacga
     8701 cgagctgctc cggaaccgtg aggggatcgt gctccgcctg cctgaggatc gcgcgcacgc
     8761 gctggccgcg cgccaggctc tggcgggtgg cctcgtcgag ccgcgtgccg aagcgggcga
     8821 aggtctcgag ctcctcgaac tgcgcgtagc cgaggcggag atcgccggcg acggcgcgca
     8881 gtgccggaag ctgcgccttg ccgccgacgc gcgagaccga cttgccgata tcgaccgcgg
     8941 ggagctggcc tttgcggaaa agctcgggcg agagatagat ctgcccgtcg gtgatcgaga
     9001 tgaggttggt cgggatgtag gcggagaggt tctgcgcctg ggtctcgatg atcggcagcg
     9061 cggtgagcga gccgccgccg agctcctcgc gcaggtgggt cgaccgttcg agcaggcgcg
     9121 aatggatgta gaagatgtcg cccggatagg cctcgcggcc gggcgggcgg cgcagcagca
     9181 gcgacagctc gcgatacgcc cgggcatgcc gcgtcaggtc gtcgtagacg atgaggacgt
     9241 cctggccctg ctccatgaag tactcggcga tggtcgtcgc cgcgtagggg gcgacgaatt
     9301 gcagccccgc cggatcgtcg ccggcggcga cgaccaccac gcagcgctcc agcgcatcgt
     9361 gctccttcag caccgccacg gcccgcgcca cggcggcacc acgctggccg atggcgcagt
     9421 agatcgagac catgccggtc cggcgctggt tgatgatggc gtcgagcgcg atggtcgtct
     9481 tgccggtctg ccggtcgccc aggatcagct cgcgctggcc gcggccgatc gggatggcgg
     9541 catcgacggc cttgatgccg gtctgcaagg gctcggcgac cggggcgcgc gccatgatcg
     9601 gcggggccgg acgctcgatc ggccagcgcg cctcggcggc gatcggctcg tcgcgatcga
     9661 gcggccggcc ggtggcatcg accacccggc cgagcagcgc cgggccgacg ggcatgtcga
     9721 cgacccggtg cgtgcgcgtc gccggatcgc cggtgcgcag cgtgcccccc tggccgagga
     9781 ggatgatgcc gatgccgtcc atggccagat tggtggcgat ccccaggagc tggttcggca
     9841 gggcgatcag ctcgctgtcc cgcacctcct gcagccccgt cacccgggcg atgccgctcg
     9901 ccacctggac gaccgtgccg acctccgaga gctgcggtcg ggcacggtag ccctgcacgg
     9961 cccggtccag cggatcgaag gtggcgtccg tcacggcgcc gagctccgaa gtgtcggccc
    10021 tgtccgtcat gcgccggcga gctccttccc gaccgccgcc tcgaagccgt cgagatagct
    10081 ggccaggctc cagctcagcc gctggctgcc ggcctccagc acgacgccgc aggcgaggcc
    10141 cgcgtccacg gtgtaggcga cctcgatccc gggatcgagc cgctcgtgca cggcacgatc
    10201 gatctgccgc cgtgcctccg cgcccagctc gaaccggctg cgcacccgga cccggccgtc
    10261 tgcggctgcg gtgatcgccg cggcggcggc gtcgtccagc ccggcgagct ggcgggcgaa
    10321 accgagcgcc acctggtcct cgagacccgt gtcggccaga tcgtcgagcg cccgcctcgc
    10381 cagggtgtag aaggcctcgg tcgagcggtg gcgcaggttg cgcagcaccg tctcccgctc
    10441 agtggccagc tgctgcagcc attcctgctt gcgcgcctcg acggcgcttc gcgcttcctg
    10501 ctcgagcgcc ttgcgctcct tgtcggcggc ctcgtgcgcc tcgcgcagca ggcgttcgcg
    10561 ggcctgctcg agtgcggccc gctcgtcgcg aaagctccgg gcttccctgt cggccgccgc
    10621 ctgcttttcc gccgcaaggc tcagccgctc ggcgatgcgc gcctcgcgtc gcgccatggc
    10681 ggcggtgatc gggccataga ggaaacgctt gagcagccag accaggacga ggaaattgac
    10741 gatctgcgct gcgaccgtga gccagtcgat ctgcatggct cacccggcgc cctggctgat
    10801 cacgtagttc cagaacgggt tggcgaagat caggatcatg gccacgacga agcagtagat
    10861 cgcggtcgat tcgatcatcg cgaggctgac gaagagtgtc cgcgtgatcc ggttcgcctc
    10921 gtccggctgc tgggcgatgg cgctgagcgc ctgggcggcg gcccggccct cgccgagggc
    10981 cgggccgatg gagccgatgg cgatggtgat gccggcggtg aagatcgaga ccgcggcgat
    11041 gagcgcgatg ctgtccatga atgggctccg ttcagctcgg ttggttcggc tcgtgccggt
    11101 cggaagccgc cgccccggcg gcctcgacct gggctgccga ggcgatgtag accatcgcca
    11161 ggatggcgaa gatgtaggcc tggatgatgc cggtcagcag gccgagcagc tccatcacga
    11221 tcgggaagaa gaaggggacg acgccgatca ggatggcgat gatgatggtg ccgctcatga
    11281 cgttgccata caggcgaacc gccaaggcca gcgtccgcga gagctcgccg atgatgttga
    11341 acggcagcat gaagaaagtc ggctgcagat actggcgcag ataggcggcg acgccgttct
    11401 cgacgatggc gtagagcggc acggcgacga gcacgcagat ggcgagggcc gtggtggtcg
    11461 agagcgagcc ggtcggcggc cggaagcccg ggacgatgcc gaggatgttc gagctcgcga
    11521 tgaagacgaa gagcgtgccg acgaagggca gatagcggcc gggctcggtc tggctcacct
    11581 cgcggatctg gtcgcccatg ccctcgacga ggacctcgag caggttctgc cagcgtgaca
    11641 tcgtctcggt gtcccgcagg cggcgggtga cgagccagct gccggcggtc agcagcagca
    11701 tgaccaccca ggtgaagagg atggtggcgt tgatggcgac tggcccgaga tggaaataga
    11761 cgatctggtc cggtgagatt tccattgcct aggtgctgcc gcgggaggtc gagagggtcg
    11821 tgcgcgccca cgtcgtgacg gcgatgcgca tgacgatgaa gccggcgagg gcggcgagca
    11881 tgagctgcca ctcgccaccc atgacgacga cgatggcgcc gacgagcagc gcgatgcgga
    11941 gcgccgcgcc gatcaggagc cggggcaggg gtgccgccga cgagccgagc cgccggacgg
    12001 agacccagag cgacgcgaga taggcagcgc cagccaagag gccgaagccg aaggccaggg
    12061 ccagggcggc aggatcccag gccatcgcca aactaggctc agtcacgccg gttctcccgt
    12121 tcgacccagt accaggcatt gatggagccg agcacggcgc cgatgaagag cagggagagg
    12181 gtccaggaga agcggctcgg cacggcaccg tccagccaga tgccgagcgc tatgccgagc
    12241 agcgtcggca cggccaccga ccagccgacc agcccgaaca tgccgagccc gaaccagacc
    12301 ccgcgatgcc gcgcgccctg cgcctcgagc ttgcgctcgg ccttgcggcc gatggcctcg
    12361 ccgaccgtct cgtcgtgtcg cgaggggcct tcggggcccg ccatcagccg atctcctgca
    12421 attcgaggaa gcgccgcacc accccgagct cgagccgggc gagcgtgctg cgtgccacct
    12481 tctggccctc gtccagctcg acatagcgcc gctggacggt cgcccggagg ctttcgagat
    12541 cgtcgcccac gaccgcgctc tgggtcgaga ccaggacctc gccggcgcac ttgacgagga
    12601 tgccctcgtc gatgccgaga tagcgctcgc ggccttcggc gtcggtgtag atcaggatgc
    12661 ccgggcggag cgccgcggcg aagtcgacgt ggcggggcag gaggccgaag gagccgttct
    12721 cggcctcggc gaccacccgg gtgaccgggc ggtcgaccag cacctcggtc ggcagcagca
    12781 cctcgagccg catgagcccg gcgccgtctc gctggtcagg cggcatcggg ctggcctcgc
    12841 gccaggcgct gctcgaggtc gtcgagcgcg ccgatcatgt agaaggcgct ttccggctgg
    12901 tcggcgaacg catcgttcag gatgcgctcg cagccgtcga gcgtgtcgtc gagcgccacc
    12961 agcctgccgg gcttgccggt gaaggcctcg gtggagaaga agggctgcgt caggaagcgc
    13021 tcgaggcggc gcgcgcggct gacgatctgg cggtccttct cggccagctc ctcgagcccg
    13081 agcatggcga tgatgtcctt gagctcctcg tactcggcca gcgtctggcg gacctcgccg
    13141 gcgatcctgt agtggcgctg gccgacgacg cccggggtca gcatcttcgc attcgactgc
    13201 agggggtcga tggccgggta gaggccctgg ctcgcacgct tgcgcgacag cgcgatcgac
    13261 gccgagagat gggcgaaggt gtgggtcgcc gccgggtcgg tgaagtcgtc ggccggcacg
    13321 tagaccgcct ggatcgaggt gatcgccccc gaagccgtgc tggagatgcg ctcctccagc
    13381 gccgcgagct cggtgccgag cgtcggctgg tagccgaccc tggacggcat ccggccgagc
    13441 aggcccgaga cctccgagcc ggcctggacg aagcggaaga tgttgtcgat cagcaccagc
    13501 acgtcctggc gggcgtcgtc gcggaaatgc tcggcgatgg tcatcgcact gtggccgacg
    13561 cgaaagcgca cgccgggcga ttcgtccatc tggccgaaga tcatcaccgt cttgtcgagc
    13621 acgcccgcgg tctgcatctc gcggtagagg tcctcggcct cgcgacagcg ctcgccgatg
    13681 ccgcagaaga ggctgacccc ctcgtacttg ccgaccatgt tgtggatcat ctcggtgatc
    13741 agcacggtct tgccgacccc cgcaccgccg aagagcccgg ccttgccgcc gcgctcgagc
    13801 gggcagagca ggtcgatcgc cttgatcccc gtctcgaaga tctccgaccg caccacgcgc
    13861 tgccgaagcg gcaccggccg ggcatggatc gagcgccgct cgacgccctc gagcggcggc
    13921 ttgccgtcga tcacctcgcc gaacatgtcg agcatccgcc cgagcacggc ccggccgacc
    13981 ggcacctgga tcggcgtccc ggtgtcgctg acgcgcatgc cgagcgccag gccgtgcggg
    14041 gcacgcagcg ccaggcccct gaccgtgcgc gggtcgagca ggcccatgac ctctatgatc
    14101 gcgtcctgat ccgggccggc gcggagcatg tgatcgatca tcggcaggtc gtgctcgaag
    14161 cgcacgtcga ccaccgtccc ctggatcgcc acgatccggc cttccgctgg ccggccttcc
    14221 cgcgtgctgg cgtgcccgtc ccgcatgctg tctccgcttc acgcgcgcca gggtccggca
    14281 cgcgcgatct gcctcgcatc tggcacaact gcctatcctg acctcgatcg aggcgcctgt
    14341 cgagcggcgg ccgccggcag aagccggctc ggcagtgctg cccgcaccgc cgggctcttc
    14401 agaatctggc cgatgaccag cggcacgagg ctgccgatcg cgatcagcag ccagccggtc
    14461 gcgcccggat cgaccgtttc cagcacgtct gccaagagcg gcacgtagag cgcggcggcc
    14521 aggagcgcga tgccgacgcc caacgctccc cagacccagg gattggtggt gaccgtgttg
    14581 cgcagcaggt cggagcccgg ctcgcgcatg ttgaagacgt gccagagccg gccgagggcg
    14641 aaagtcagga acgagatggt caccgcctgc tccgtcgcca tgccggcgac caggagagca
    14701 taggcgaaga ccgccatgac ggagctgccg atcagcacgc cgtagccgcc gatggcgagc
    14761 cagtggcgac gcgcgacgat cggctcgtcc gggtcgcggg ccggtcgatc gagcgggctc
    14821 tcgccccgcc cgacgccgag ggcgagcgcc gggaacacgt cggagatgag attgatgtag
    14881 aggatctgca gcggcagcag cggcaggggc gcgttgagca gggcgaggac cgccaccgcg
    14941 aagatctcgc cggcattgcc cgacagcatg tagacgatga accgccggat gttgtcgaag
    15001 atcacgcggc cctgggcgat cgccaggacg atggtggcga agctgtcgtc gcgcagcacc
    15061 atgtgcgccg cctcgcgggc gacctgggtc cctcgctggc ccatggcgat cccgatgtcg
    15121 gccttcttca gggccggcgc atcgttcacg ccatcgccgg tcatggcgac cctggcaccg
    15181 tcctcctggt gaatgcggat gaggtcgagc ttctgcttcg ggtcgacccg ggcgaggatc
    15241 gaggcctcca gcacccgccg ccgctccgcc tcggacatcg cctccacttg gccgagatcg
    15301 ccgccgtgga cgatcggcac gtcgtcgccg gcatcgacga gcccgaccgc ctcggcgatc
    15361 gcccgggccg tcgccggctg gtcgccggtg accatgacga ccctgatccc ggcctcgcgg
    15421 caggcacgga tggcgtcgcg cacgtcgcgg cgcggcggat cgaggagccc gacgaggccg
    15481 atcagctcga gatcctggta aggctggtcg tcggcgctgg tcgccgactt ctccgccagc
    15541 gccagggcgc gcaggccgct ccgggcaagc gcttcgttgc gttcgcgcca tcgcgcgcgg
    15601 ccggcctcgt cgagcgcctc cgcctcctcg ccggagcgga cccgcgtgca ggcctcgatc
    15661 accgcctccg gcgctccctt gacggtgacc ctgacgcccc cctcggcctg gtggaaggtc
    15721 gccatcatcc tggtcgccgg ctcgaactcc tcgcggcgca gctccggctg ccgctcgagc
    15781 agcgcctcgc ggtcgatgcc cgcggcggcg gcaccggcga ggagtgcgag ctccatcgga
    15841 tcgcccttgc cgccgcggtc gttctccgcc agctcggcgc tggtgcagag cgcgccgatc
    15901 tcgagcgcct ttcgcagggc cgggtcctcg gcggcatcct cgccgtcccg ccgcaagcac
    15961 ggcggctcgt cgtcg
//
LOCUS       DAOTFI010000111        15783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4480_length_15783_cov_7.336279, whole genome shotgun sequence.
ACCESSION   DAOTFI010000111 DAOTFI010000000
VERSION     DAOTFI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 15783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15783
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4480_length_15783_cov_7.336279"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="P5558_12575"
     CDS             <1..595
                     /locus_tag="P5558_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25192.1"
                     /translation="RAHSALLGIGLYLDSTGEVPSDQMRVTYTDEAKMREVYEKLLAY
                     AVERKDRVIQFWSNAQETEAAFLQNGAVIGQTWDGPAMRMRTDSGGRYTYLAAKEGAL
                     TWMDSMGIPTGAENLEQAYAWMNWYYQPDNAAIHVRLSGYNSCTEGAAELAGEDYAAN
                     FAAAYPGNAIENLWWYPAEPTWFVAARNEFRDRFLSA"
     gene            657..1745
                     /locus_tag="P5558_12580"
     CDS             657..1745
                     /locus_tag="P5558_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25193.1"
                     /translation="MLAGQSVTLERVSMAFGDFVAVRNADLHIEAGEFFSFLGPSGCG
                     KTTILRLISGFNEPTGGAIRIGGADMAGIGPNRRPTALIFQNLALFPLMKVWENVAFG
                     LEARGVARAERRRRAAELLELVALEGQGEKLVSALSGGQRQRVAIARALAVEPAVLLL
                     DEPLSALDLKLRQKMRAELRTIQQKTGVTFVYITHDQGEALTMSDRIAVMSQGRIEQV
                     GTADAIYREPASAFVATFVGESNVLAGRVTGTLGDEAMIETAHGGLAGRNPHGLRTGV
                     AAQLVVRPERCRLLDGDAPGNRLAGTVERLDFEGAFVSLVVRLADGTEVTAQLANDGS
                     LPRLKAGTPAAIGFPPEAALLLPVAGDG"
     gene            1738..2631
                     /locus_tag="P5558_12585"
     CDS             1738..2631
                     /locus_tag="P5558_12585"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25194.1"
                     /translation="MVELFRRLGATAGAIVLGTVILWVLFLVVLPQLFMVDMSLRPHL
                     PVPEQGGPADVRSLANYATLFANDAHRGIFLRTIFSAALVTVLALIVCYPVAYYLAQV
                     ARGPTAALLVLGLVVPFWVNEILRTFSWFLILSRNGLLNQVLMGLGLTEAPLNMGGVG
                     AVMVGMVYAYILFMVFPLYNAIESLDRNQIEAARDLGASWPRIHARVVVPHAKPGIAV
                     GCIMTFMLAAGTVAVPQLLEALPGGTNARWFTQVIYSLFFDGGDWNSGAAYAFTLLVS
                     CVIFIFVMMRVFRVGIADVAK"
     gene            2628..3467
                     /locus_tag="P5558_12590"
     CDS             2628..3467
                     /locus_tag="P5558_12590"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25195.1"
                     /translation="MSARRLVDLFVYAYLALFFAYLFLPLVFMVAAAFNDSRFPTMLP
                     WQGFTLDWFWGFDSRGRAIGLFQDGRMGMAIWNSVLIGLGVILVSIPVGLAGALFLQT
                     IRTRAKSFLYGVMVSPILTPGVILGISTLVFWRNLFDVGGGLMLAVIAQSTFIAAYCM
                     LLFLARLERFDRTQEEAALDLGASHGLMMRRVMLPFLRPALLSGAVLAFLQSFENYNT
                     TLFVIGIEDTLTLNIATRVRLGLTPAVNAIGVIMIGLTILGAVVYEILRRREARRSAA
                     LAG"
     gene            3590..5389
                     /locus_tag="P5558_12595"
     CDS             3590..5389
                     /locus_tag="P5558_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY25196.1"
                     /translation="MAKGKAADGRPSKPRTAGSTPTERVSPLLPRYTQFDRLWHAGQA
                     RLTFGISLAAVAAAWSDWALHLANAPGRRLELAQAALHDAWRLGLFAARASSGGASEP
                     FSQDGDDPRFADPAWHRFPFNVWAQGFLAAEAWWDAATTGPRGVTAQHQRQVRFMARN
                     ALDHWAPSNLPWTNPEVLERTAAEQGRNLLQGLEFLAEDIERRAAGEGPAGTERFAVG
                     RNMAMTPGRVVYRNDLIELQQYTPTTETVYPEPVLIVPAWIMKYYVLDLEPGNSLIEY
                     LVGQGHTVYCISWKNPGEAEAELELDDYRRQGVHAALTVVNTIQPERKVHAVGYCLGG
                     TILAIAAAAMARDGDDRLASMSLLAAQTDFSEAGELMLFIDESQIAYLEDMMWEQGYL
                     DTEQMAGAFRLLRPNDLVWSRLVRQYLMGEREEMRALMAWNADGTRMPARMHGQYLRA
                     LFLDNRLSRGRYAVDGRAIALSDIRVPIFAVGTEKDHIAPWESVYKVRLLTDVPVTFA
                     LTNGGHNGGIVSRPDHPGRYYRLETMAKDAIYQPPEQWLAGSTPRPGSWWLPFQTWLA
                     EGSGKRTAPPSTGAPEKGLPPLDLAPGRYVLER"
     gene            5596..7005
                     /locus_tag="P5558_12600"
     CDS             5596..7005
                     /locus_tag="P5558_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25197.1"
                     /translation="MTKGQLVESALFGYDTFANVANYASTSALPKLPTGNAVERGWAP
                     LVLPGLHSPGVDLADIGVLVGQPAGTFTSAAHFYTGELEGRQTLVVTYRSTDETNEFG
                     FQGLEIAPGVRAWDLYYSAHQDATVAALTYAATPTNGIEQVLFAGHSLGGIIAELSVA
                     RLVGDGAPFAPLADRTLLVTFGSPGSTETVEGVAQLNVVHSDDFVAQLSALSPLFIAG
                     GATREGEDLVVERPEAQLPDFEPQDLDTPDELIAASRIPGIGDEHPITVYIDTASLLE
                     SAESFVPGVRDALDDPFRWLSAEIDRTIVATNDSEHIIGDKTAELIFALGGNDIVFGN
                     KGDDVLVGQSGHNQLFGGKGNDTLVDGSGNDILSGDKGDDLFFLNGGLNIANGGSGKD
                     AAVFDGAAGDFSVFAFGRIGVVSRIGDPGDRTIIQSIEKLEFDDQLLVLERGTFEPVP
                     EVAVASAAIDDLVIAGEIA"
     gene            7159..>7842
                     /locus_tag="P5558_12605"
     CDS             7159..>7842
                     /locus_tag="P5558_12605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY25198.1"
                     /translation="MAWGVAQAMVMDQSTHAACRVCLGGARPFMTVQGRRYWRCRRCR
                     ATQLDARQLPDPGTERRHYLTHHNDPADEGYRRFLARLASPLLKVLDRASEGLDFGCG
                     PGPALAAMLEAAGHRVRLHDPFFRPDPAALERCYDFVTLTEVAEHLHRPHAVFRQLAG
                     LIRPGGRLAVMTALGIDDRRFANWRYRRDPTHVVFYRAATFRYLASSLGLDCRRVAPD
                     VVLLRKPGSG"
     assembly_gap    7843..7942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7943..8997)
                     /locus_tag="P5558_12610"
     CDS             complement(<7943..8997)
                     /locus_tag="P5558_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25199.1"
                     /translation="MTLRSDRPERLHALDALIGVWLTLIYVVYLTHADMWIAATAWLG
                     WFEQPTRIFLMGALPLAAIWTACRLADRAGRIPRPGPLDMLEANRPVLLAFACLCAIS
                     LLPGAVLPGAVVDDGGTQLMVLPYAFVVFTFGLMFGGAVAVRRHWRRILLMTWLVTVT
                     SVLVELFRPRTFSGLLPAVNADWATDADRPAGLMGDANTAAFIMLGLTLLILRYDRLR
                     WPEVGLLAMTLLAVLATQSRGGLLLTAMVVAGYLYHCRAVIRGPALTAALLGGLAAVA
                     GVVLVVLPLLANLAAFSDWEGQRRLEMLTFQGAFVERDESRLGLASSYLAAIEEAVWL
                     GHGTGYMRTRPIGSHNM"
     gene            complement(8994..10199)
                     /locus_tag="P5558_12615"
     CDS             complement(8994..10199)
                     /locus_tag="P5558_12615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY25200.1"
                     /translation="MRAPSASSRGDPAAAGRSATGPARPIAIFLPALYAGGAERVLVN
                     LAGELHKRGHSVDLVLAHATGPYLDRVPAGVRVVDLGCRRVATALLPFARYLREVQPV
                     AVHAAIDHTNVIALLARAFVRLTGGTDTRMVVSVHTPPSVFQSLSSGLVRARGAMVGA
                     IARFAYRLADEVVAVSAGVADDVARTLHLPRERIRVIYNAALTEDPAGQGRAPLGPVP
                     IALKGRRFLVAAGRLMPSKDFANLLEAMARLRRHRDVALLILGDGPERAALEALVQKL
                     GLGADVWLAGFQANPYRFFSRAAVFVLPSRREGLPTVLIEAMACGCPIVSTDSPHGPR
                     EILADGRFGHLVPMGEPRALADAIARMLDHPTSPDLLEERLAAFATDAIIEAHLETLL
                     GTTRERCPS"
     gene            complement(10199..11656)
                     /locus_tag="P5558_12620"
     CDS             complement(10199..11656)
                     /locus_tag="P5558_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRY25201.1"
                     /translation="MMLRHGLAYLAARGLPALLNFAALAIYTRLLAPEQFGIYAMVLA
                     GLGIAHTAFLSWLDLGLLRFLAAYEVRRGTFLGTILIAFLAMLALAGLASLLALWLTS
                     DPLLDRLLPLGFLLFCVEGFAELHLRLANAQLTPAVYGRMAITRALVALAVGSALVVA
                     GAGAEGILLGLAAGAVAAVVAPTRRELGSLWSAWRQVATPVFDPAVLRHLFLYGAPLV
                     VTLGLDVVLAGADRFLLARVIGTDAAGVYAVGYQLTAYTLGTLLVIVNLAGYPLAVKA
                     FETGGLAAAEPYLRQNALLLWGIGLPAACGMGLLAPNITAVVTGSAFAADAALVMPVL
                     VLAALLQRCRTYYFDFAFQLRAKVAWQALISAATVILNIGLNLLLIPRFGILGAAWAT
                     VAACGLGLALSIVIGRRVLALPLIWGEALRVCLATTVMLTALWPLRELSGLLALVLQI
                     VLGGLAYAAALWLLDVGGLRAGAGRLLARQPAGHT"
     gene            11752..13284
                     /locus_tag="P5558_12625"
     CDS             11752..13284
                     /locus_tag="P5558_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRY25202.1"
                     /translation="MPSRIDPSVPAAGRPVSKADLRRNFAAAQGELEHLGWLRQPYAG
                     AVERTGAAKLAETLSAADFGAIGDGSTDDTAALQAALDALKRQGGVLALTPGAVYRIS
                     RGLLLEDAAGFRIEGRDARLVMADGAPVAVHYWLLQLGRCRRFTIADLRLDGNRQTRA
                     IGPEPWANNLQLHDCSDFVLQSVECSNAVVDGFILYHGRRDAPATFCRRGLMLGCRAV
                     NAARQGLTILNGHDIRVVACDFIGSNGVPPEAGIDIESHIDSAAPSNARVLIQGCHFE
                     DNRGGGILLTALGGAHGFSVVDCSFASCGPYGVDIVAPDCTVRGCRFTRFGPEVRHGV
                     VNIRTREGGLPALVSGNSFDDILIGDANRAAIYVRPDASPGTLVIDNRLNAVDASGII
                     AAAPGTIVRGNVIEGNQLEGIRVGHAALVEGNRIALMRPGRPAILVHGGRTVVRGNHL
                     IDCDNGMEGTILVLDDGTGDSRPPVLDGNYLSLSGGDGRSVPVRLRAAAIEAGTTAFG
                     YG"
     gene            complement(13296..14570)
                     /locus_tag="P5558_12630"
     CDS             complement(13296..14570)
                     /locus_tag="P5558_12630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY25203.1"
                     /translation="MEELPTAPPVHATGTPARALPITGPRPAPLAATVVHVATNLELG
                     GAQRMLLKLVEAGVSAATTTHVVTLMPAGALGPALAATARLHSLDLARGGFSPMAAVR
                     LASLLRQLQPDLVQGWMYHGNLAASVGLALARGLERRCARAALLWNIRGALDAPERER
                     WLTRQIVRLGARLSGRPEAIVYNAEVAARQHEAIGYRAQRRQVIGNGFDPDRFAPDPE
                     AKGNLRARLGLGPNSVLIGIAAEWRPMKDHATLLAAMRRLVDFGLDVDLVLLGAGTAA
                     DSPTLVHHVAANGLGGRVHGLGPTLDAAPVIAGFDIAVLASAWGEGFPNFIGEAMASG
                     VPCVATDSGDCRRVIGAAGQVVPVRDPAALADALATLVRTSAGERVRLGRLGRERILS
                     HYSMTAIAAEYRSLYAECIARRGVRAVPGADG"
     gene            complement(14579..15769)
                     /locus_tag="P5558_12635"
     CDS             complement(14579..15769)
                     /locus_tag="P5558_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY25204.1"
                     /translation="MTAAADQGVLDPSPDRTRTTTPGRVFLSPPHMAGDELALLHEVF
                     ATNYVAPAGPMLSRFEAAMAALTGFHHCLAVVSGTAALHLALRHLGVGPGDRVIAADL
                     TFIGGVAPIGQLGAEPIFVDADPETWCMNPGLLAEALAGGARQGRLPKAVVPCDLYGQ
                     ACDLDALRAVTDAYEIPLIADSAEALGATYRGRHAGKGARAALYSFNGNKIVTAGGGG
                     MLASDDAALIEHARGLSQQARQPVPHYEHVELGYNYRLSNVLAAIGVGQLAVLDDRVR
                     QRRAIFSGYQQRLGRLDGITFMPEAGYGRANRWLTCILIEPRAFGADREAVRLALEAQ
                     DIEARPLWRPMHMQPVFAGATCFGGGVGKALFDQGLCLPSGSALGDGDLDRICEVIRG
                     CARP"
BASE COUNT         2475 a         5440 c         5416 g         2352 t
ORIGIN      
        1 ccgtgcacac tcggccctgc tcggtatcgg cctctatctg gattcgaccg gtgaggtgcc
       61 gtccgaccaa atgcgcgtca cctacaccga cgaggcgaag atgcgcgagg tctacgagaa
      121 gctcctcgcc tatgccgtgg aacgcaagga ccgggtcatc cagttctggt cgaacgccca
      181 ggagactgag gcagccttcc tgcagaacgg cgccgtcatc ggtcagacct gggacggccc
      241 cgccatgcgg atgcgcaccg attccggcgg ccgctacacc tatctggcgg cgaaggaggg
      301 ggcgcttacc tggatggact cgatgggcat cccgacgggt gccgagaatc tcgagcaggc
      361 ctatgcctgg atgaactggt actaccagcc cgataacgcg gcgatccatg tgcgtctctc
      421 gggctacaac tcctgcaccg agggtgcggc cgagctcgcc ggcgaggact acgccgccaa
      481 cttcgccgcg gcctatccgg gcaacgccat cgagaacctg tggtggtatc cggccgagcc
      541 cacctggttc gtcgctgccc gcaacgagtt ccgcgaccgc ttcctctcgg cctgaggccc
      601 gcgcgcccgg ccggcgaccg cttcgccggc cgggccgcac agccggaaaa ttgccgatgc
      661 tggcgggaca gagcgtcacc ctcgaacggg tgagcatggc gttcggcgat ttcgtcgccg
      721 tcaggaatgc cgacctgcac atcgaggcgg gcgaattctt ctccttcctc ggcccctcgg
      781 gctgcggcaa gaccaccatt cttcgcctga tctcgggctt caacgagccg accggcggcg
      841 ccatcaggat cggcggcgcc gacatggccg gcatcggccc gaaccgccgg ccgacggctc
      901 tgatcttcca gaatctcgcc ctcttcccat tgatgaaggt ctgggagaac gtcgccttcg
      961 gcctcgaggc gcgaggcgtc gccagggccg agcgccggcg gcgcgccgcc gagctgctcg
     1021 agctcgtggc cctggaaggg cagggcgaga agctggtctc cgccctctcc ggcggccagc
     1081 gccagcgcgt cgccatcgcc cgcgcgctgg cggtggagcc cgccgtcctg ctgctcgacg
     1141 agccgctctc cgccctcgac ctcaagctcc ggcagaagat gcgcgccgag cttcgcacca
     1201 tccagcagaa gaccggcgtc accttcgtct acatcaccca tgatcagggc gaggcgctca
     1261 ccatgagcga ccgcatcgcc gtcatgagcc aggggcgcat cgagcaggtc ggcaccgccg
     1321 atgccatcta ccgcgagccg gcctcggcct tcgtcgccac cttcgtcggc gagagcaatg
     1381 tgctggccgg gcgggtcacc ggaaccctgg gtgatgaggc gatgatcgag accgcccatg
     1441 gcggccttgc cggccgcaac ccgcacggcc tcaggaccgg tgtcgccgcc cagctcgtcg
     1501 tgcgccccga gcgctgtcgc ctgctcgacg gcgacgcccc gggcaaccgg ctcgccggca
     1561 cggtcgagcg gctcgacttc gagggggcct tcgtcagcct cgtggtccgg cttgccgacg
     1621 gcacggaggt cacggcccag ctcgccaatg atggcagcct gccgcgcctg aaggccggca
     1681 cgcctgccgc catcggcttc ccgccggagg cagcactcct cctgccggtg gcgggcgatg
     1741 gttgagctct tccgccggct cggggccacg gccggtgcca tcgtgctcgg cacggtgatc
     1801 ttgtgggtgc tcttcctcgt cgtcctgccg cagctcttca tggtcgacat gtcgcttcgc
     1861 ccgcatctgc cggtgcccga gcagggcggg ccggctgatg tccgcagcct cgccaactac
     1921 gccaccctct tcgccaacga tgcgcatcgc ggcatcttcc tgcgcaccat cttctcggcg
     1981 gccctggtca ccgtgctcgc cctcatcgtc tgctaccccg tcgcctacta cctggcacag
     2041 gtggcgcggg ggccgacagc ggcgctcctg gtcctcggcc tcgtcgtgcc cttctgggtc
     2101 aacgaaattt tgcgcacctt ctcctggttc ctgatcctct cgcgcaacgg cctcctgaac
     2161 caggtgctga tgggcctcgg cctcaccgag gcgccgctca acatgggtgg ggtcggggcg
     2221 gtgatggtcg gcatggtcta tgcctacatc ctgttcatgg tcttcccgct ctacaacgcc
     2281 atcgagagcc tcgaccgcaa ccagatcgag gcggcccgcg acctcggcgc ctcctggccc
     2341 cgcatccatg cccgggtcgt cgtcccgcac gccaagcccg gcattgccgt cggctgcatc
     2401 atgaccttca tgctcgccgc cgggacggtg gcggtgccgc agctgctcga ggcgctgccc
     2461 ggcggcacca atgcccgctg gttcacccag gtcatctaca gcctcttctt cgacggcggc
     2521 gactggaaca gcggtgcggc ctatgccttc accctgctcg tctcctgcgt catcttcatc
     2581 ttcgtcatga tgcgcgtctt tcgcgtgggc atcgcggacg tggcgaaatg agtgcgcgac
     2641 gcctggtcga cctcttcgtc tatgcctacc tcgccctctt cttcgcctat ctcttcctgc
     2701 ctctggtctt catggtggcc gccgccttca acgacagccg ctttcccacc atgctgccct
     2761 ggcagggctt caccctcgac tggttctggg gcttcgacag tcgcggccgc gccatcggcc
     2821 tcttccagga cgggcgcatg ggcatggcga tctggaactc ggtgctgatc ggcctcggcg
     2881 tcatcctggt ctcgatcccg gtcggcctcg ccggcgcgct cttcctccag accatccgaa
     2941 cccgggccaa gtccttcctc tacggcgtca tggtctcgcc gatccttacc ccgggcgtga
     3001 tcctcggcat ctcgaccctg gtcttctggc gcaacctctt cgatgtcggc ggcggcctca
     3061 tgctcgcggt gatcgcccag agcaccttca tcgccgccta ttgcatgctg ctcttcctcg
     3121 cccggctgga gcgcttcgac cgcacccagg aggaggccgc cctcgacctc ggcgccagcc
     3181 acggcctgat gatgcgccgg gtgatgctgc ccttcctccg ccccgccctg ctctcgggtg
     3241 ccgtgctcgc cttcctccag tccttcgaga actacaacac caccctcttc gtcatcggca
     3301 tcgaggacac gctgaccctc aacatcgcga cgcgcgtgcg cctcggcctg accccggcag
     3361 tcaacgccat cggcgtcatc atgatcgggc tcaccattct gggtgcggtc gtctacgaga
     3421 tactccgccg gcgtgaggcc aggcggagtg cggcgctcgc cggctgagag gagctcgaac
     3481 ggtcgactgg cgaccttggc ggctggtgcg accctggcgc cgccttgcat cgcccgtctc
     3541 ctgtcgccac ttcgtgttgc aatgcaccat gcggtcgctc gggggaaaca tggcgaaagg
     3601 caaggctgct gacggccgac cgtcgaagcc acgtacggcc ggatcgacgc cgaccgagcg
     3661 ggtatcgccg ctcctgcccc gctacacgca gttcgaccga ctctggcatg cggggcaggc
     3721 caggctcacc ttcggcatct cgctggcagc cgtggcggct gcgtggagtg actgggcctt
     3781 gcacctcgcc aacgcaccgg gccggcggct cgagctcgcg caggcagcgc tgcacgacgc
     3841 ctggcggctc ggtctgttcg ctgcgcgggc gtcgagcggc ggggcgagcg agcccttctc
     3901 gcaggacggc gacgatccgc gctttgcgga tccggcttgg catcgttttc ccttcaatgt
     3961 ctgggcgcag ggcttccttg cggcggaagc ctggtgggat gcggcgacca ccgggccgcg
     4021 cggggtgacc gcccagcacc agcgtcaggt gcgcttcatg gcgagaaacg cgctcgatca
     4081 ctgggcgcct tccaatctgc cctggaccaa tcccgaggtg ctcgagcgca cggccgccga
     4141 gcagggccgc aacctcttgc aaggcctcga gttcctcgcc gaggacatcg agcggcgcgc
     4201 ggccggggag gggccagccg gtaccgagcg cttcgcggtc ggcaggaaca tggcaatgac
     4261 accgggccgc gtggtctatc gcaacgacct gatcgagctc cagcaatata cgcccacgac
     4321 cgagaccgtg tatccggagc ccgtgctgat cgtgccggcc tggatcatga aatactacgt
     4381 gctcgacctc gagcccggca actcgctgat cgagtatctg gtgggccagg gtcacaccgt
     4441 ctactgcatc tcgtggaaga acccgggaga ggccgaggcc gagctcgagc tcgacgatta
     4501 ccgccgtcag ggcgtgcacg cggcgctcac ggtggtcaat acgatccagc ccgagcgcaa
     4561 ggtgcacgct gtgggctatt gcctgggtgg caccatcctc gcaatcgcgg ctgccgccat
     4621 ggcgcgggac ggtgacgacc ggctcgcttc gatgagcctg ctggctgccc agaccgactt
     4681 cagcgaggcc ggcgagctca tgctcttcat cgacgagagc cagatcgcct atctcgagga
     4741 catgatgtgg gagcagggct atctcgacac cgagcagatg gccggcgcct ttcgtctcct
     4801 gcggccgaac gacctcgtct ggtcgcggct cgtccggcag tacctgatgg gcgagcgcga
     4861 ggagatgcgg gcgttgatgg cctggaatgc cgacggcacg cgaatgccgg cgcggatgca
     4921 cggccaatac ctgcgcgccc tctttctcga caaccggctg agccgcggcc gctatgccgt
     4981 cgatggccgg gccatagcgc tcagcgacat ccgcgtgccg atcttcgccg tcggcaccga
     5041 gaaggaccac atcgcccctt gggaatccgt ctacaaggtg cgactcctga ccgacgtacc
     5101 ggtcaccttc gcgctcacca atggcggcca caatggtggc atcgtcagcc ggccggacca
     5161 tccgggccgc tactaccgcc tcgagaccat ggcgaaagac gccatctatc agccgcccga
     5221 gcagtggctc gccggcagca cgccccggcc cggctcctgg tggctgccgt tccagacctg
     5281 gcttgccgaa ggaagcggca agcgcaccgc tccgccatcc acgggtgcgc cggaaaaggg
     5341 cctgccgccc ctcgacctgg ccccgggccg ctatgtgctg gagcgttgag caacgagtcg
     5401 gtcagccgcg ccgaccgcgc gatcgttgtc gaccggatca aatattcttt ccggtgttga
     5461 tttgcatcaa tccggtggcc gacccatcaa gggataattc ctggtccgcg ttcgcaagat
     5521 ctgggtcgca agagccaggg tggcgggccg atgtggttga tttgaagcac aacatccttt
     5581 cgatagggta ttccgatgac caaaggacag ctcgtcgaat ccgctctttt cggctatgac
     5641 acgttcgcca atgtagccaa ctatgcaagc acttcggccc tgccgaagct gccgactggc
     5701 aatgcggtgg agcgaggctg ggcgccgctc gtcttacctg gcctgcacag ccccggcgtc
     5761 gatctggcgg atatcggcgt cctggtcggc cagccggccg ggaccttcac cagcgccgca
     5821 catttctaca ccggcgagct cgagggccgc caaaccttgg tggtcaccta ccgcagcacc
     5881 gacgagacta acgagttcgg ctttcagggg ctggagatag cacccggtgt ccgtgcctgg
     5941 gacctctatt acagtgcaca ccaggacgcc acggtggccg ctctcaccta cgccgcgacg
     6001 ccgacgaacg ggatcgagca ggttctcttc gccgggcaca gcctcggcgg catcattgcc
     6061 gagctcagcg tcgctcgact ggttggcgac ggcgccccgt tcgctccgct cgccgaccgg
     6121 acgctgctcg tcaccttcgg ctcgcctgga tcgaccgaga cggtcgaggg cgtggcgcag
     6181 ctcaatgtcg tccactcgga tgatttcgtg gcgcagctct cggcgctgag cccgctcttc
     6241 atcgctggcg gtgccacgcg cgagggcgaa gacctggtcg ttgagcggcc ggaggcgcag
     6301 ttgccggatt tcgagccgca ggatctggac acgcccgacg aactcatcgc cgccagccgg
     6361 atcccgggca ttggcgacga gcacccgatc accgtttata tcgacaccgc cagcctgctc
     6421 gaatcggccg agagcttcgt gcccggtgtg cgcgacgcgc tcgacgatcc gttccgctgg
     6481 ctgagcgcgg agatcgatcg gacgatcgtc gccaccaacg acagcgagca catcatcggc
     6541 gacaagaccg ccgagctgat cttcgccctt ggcggaaacg acatcgtctt cggcaacaag
     6601 ggcgatgatg ttctggtggg ccagtcgggc cacaaccagc tcttcggcgg caagggcaac
     6661 gacaccttgg tcgacggctc gggcaacgac atcctgagcg gcgacaaggg cgatgatctc
     6721 ttcttcctga acggtggcct taacatcgcg aacggcggca gtggcaagga tgcggctgtc
     6781 ttcgacggcg ccgccgggga cttctcggtc ttcgctttcg gcaggatcgg ggtggtcagc
     6841 cggatcggcg acccgggcga tcgaaccatt atccagagca tagagaagct ggagttcgac
     6901 gatcagctgc tcgtgctcga gcgtggcacc ttcgagcctg tgccggaggt cgccgtcgcg
     6961 agcgcggcaa tcgatgatct ggtgatcgcg ggtgagatcg cctgaagctc gagcgacgtg
     7021 acgtccggcg cttgtcgaag ctcggctgac cgcgccacgt tggtcgcccg gatccatgcc
     7081 ggggctgttg cggtgcggtc ggcgagccga tgatgccgac cgaagcgagc atcgcgctgg
     7141 tccctggcgg ctggtgccat ggcgtggggg gtggcacaag cgatggtcat ggaccagtcg
     7201 acccatgcgg cgtgccgggt ctgcctcggc ggtgcccggc ccttcatgac ggtccagggc
     7261 cggcgctact ggcgttgccg gcgctgccgg gcgacgcagc tcgatgcgcg ccaactgccc
     7321 gaccccggga ccgagcgccg gcactacctg acacatcaca acgatccggc cgatgaaggc
     7381 tatcgccgct tcctcgcccg gctggcctca ccgctcctga aggtgctcga ccgagcgagc
     7441 gagggtctcg atttcggctg cggcccgggc ccggcgctgg cggcgatgct cgaggcggcc
     7501 gggcaccggg tcaggctcca cgatcctttc ttccggcctg atccggcagc actcgagcgg
     7561 tgctacgact tcgtcacgct gaccgaggtg gccgagcatc tgcaccgacc acatgctgtc
     7621 ttccgccagt tggccggcct gatccggccc ggcggccggc tcgccgtcat gacggcactc
     7681 ggcatcgacg accggcgctt cgccaactgg cgctaccggc gcgaccccac acacgtggtc
     7741 ttctaccgcg ccgccacctt ccgctacctg gcctcgagcc tggggctcga ctgccggcgc
     7801 gtcgccccgg acgtcgtgct gctgcgcaag cccggctccg gcnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nntacatgtt gtggctgccg atcggccggg tgcgcatgta
     7981 cccggtgccg tggccgagcc agacggcttc ctcgatggcc gcgagatagc tcgaggccag
     8041 ccccagccgg ctctcgtccc gctcgacgaa ggcaccctgg aaggtcagca tctccagccg
     8101 gcgctgccct tcccagtcgc tgaaggcggc gaggttggcg agcagcggca gcaccaccag
     8161 caccacgccc gccacggcgg cgaggccgcc gagcagggcc gccgtcaagg cgggcccgcg
     8221 gatcaccgcc cgacagtggt agagatagcc cgcgaccacc atggcggtga gcagcaggcc
     8281 gccgcgcgac tgggtggcga ggaccgcgag cagggtcatg gcgagcaggc cgacctcggg
     8341 ccagcgcagc cggtcgtagc gcaggatgag cagggtgagc ccgagcatga tgaaggcggc
     8401 ggtattggcg tcgcccatga gcccggccgg ccggtcggcg tccgtcgccc aatcggcatt
     8461 gaccgccggc agcaggcccg agaacgtgcg cggccggaag agctcgacga gcacgctggt
     8521 gacggtcacg agccacgtca tgagcaggat gcggcgccag tgccggcgca ccgccacggc
     8581 accgccgaac atcaggccga aggtgaagac gacgaaggca tagggcagca ccatgagctg
     8641 ggtcccgcca tcgtcgacga ccgcccccgg caggacggca ccgggcagca gcgagatggc
     8701 gcagagacag gcgaaggcca ggagcacggg ccggttggcc tcgagcatgt cgagcgggcc
     8761 gggcctcggg atccggccgg cccggtcagc cagccggcag gcagtccaga tggcggccag
     8821 cggtagcgcg cccatgagga agatccgcgt cggctgctcg aaccagccga gccaggcggt
     8881 ggcggcgatc cacatgtcgg catgggtgag atagacgaca taaatgaggg tcagccagac
     8941 cccgatcagg gcatctagcg catgcaggcg ttccgggcgg tcggaccgca gcgtcacgac
     9001 gggcacctct cgcgggtggt gccgagcagc gtctcgaggt gggcctcgat aatggcgtcg
     9061 gtggcgaagg cggcgagccg ctcctcgagc agatcgggcg aggtcggatg gtcgagcatc
     9121 cgcgcgatcg cgtcggcgag ggcccggggc tcgcccatgg ggacgagatg gccgaagcgg
     9181 ccgtcggcca ggatctcgcg cgggccgtgc ggcgaatcgg tcgagacgat cgggcagccg
     9241 caggccatgg cctcgatcag cacggtcggc aggccctcgc ggcgcgacgg cagcacgaag
     9301 acggcggccc ggctgaagaa gcggtagggg ttggcctgga agccggcgag ccagacgtcg
     9361 gcgcccagtc cgagcttctg gaccagtgcc tcgagagccg cccgctccgg cccgtcgccc
     9421 aggatcagga gtgccacgtc gcggtgccgt cggagccgtg ccatggcctc cagcagattg
     9481 gcaaagtcct tgctcggcat cagccgcccg gccgccacga ggaagcgccg ccccttgaga
     9541 gcgatcggca ccgggcccag cggcgcccgg ccctggcccg ccggatcctc ggtcagcgcc
     9601 gcgttgtaga tcacccggat ccgctccctc ggcagatgca gcgttcgggc gacatcgtcg
     9661 gcgacaccgg ccgagacggc gaccacctcg tcggcgagcc gataggcaaa gcgggcgatg
     9721 gcgccgacca tcgcaccgcg tgcccggacg agcccgctgg agagggactg gaagacggac
     9781 gggggtgtgt ggacgctgac caccatccgc gtatccgtgc cgccggtcag ccggacgaaa
     9841 gcccgggcga gaagggcgat cacattggta tgatcgatcg cggcatgcac cgcgaccggc
     9901 tgcacttcgc ggaggtagcg tgcgaaaggc aggagcgccg tggccacccg ccggcagcca
     9961 agatcgacca cccggacgcc ggctggcacc cggtcgagat aggggccggt ggcgtgcgcc
    10021 agcaccagat cgacgctgtg gccacgcttg tggagttcgc ccgccagatt gaccagcaca
    10081 cgttcggcac cgcccgcata gagggcgggc aggaagatgg cgatcggccg ggcagggccg
    10141 gtcgcggaac ggccggctgc cgccggatcg ccgcggctcg aggctgaagg agctcgcatc
    10201 aggtgtgacc cgcgggttgt cgtgccagga ggcggccggc cccggcccgc aggccgccga
    10261 cgtcgagcag ccagagggcg gcggcatagg ctaggccgcc gagaacgatc tgcaggacca
    10321 gcgcgaggag gccggaaagc tcgcgcaagg gccagagcgc cgtcagcatc accgtcgtgg
    10381 cgaggcagac tctcagcgcc tcgccccaga tgagtggcag ggcgaggaca cgccggccga
    10441 taacgatgct cagcgctagg ccgaggccgc aggcggcgac cgtggcccag gccgcaccca
    10501 ggatgccgaa gcgcgggatc aggagcaggt tgaggccgat gtttaggatg acggtcgcgg
    10561 ccgagatcag tgcctgccaa gcgaccttcg cccgaagctg aaaggcgaag tcgaaataat
    10621 aggtgcggca gcgctggagc agggcagcca gcaccaggac cggcatgacc agggcggcgt
    10681 cagcggcgaa ggcgctgccg gtcaccacgg cggtgatgtt cggggcgagc agccccatgc
    10741 cgcaggcggc cggcaggccg atgccccaga gcagcagcgc gttctgccgc agatacggct
    10801 cggccgcggc gagaccgccg gtttcgaagg ctttgaccgc cagcgggtag ccggcgagat
    10861 tgacgatcac caggagcgtg ccgagcgtgt aggcggtgag ctgatagccg accgcgtaga
    10921 cgccggccgc atcggtgccg atcacccgtg ccagcaggaa ccggtcggcg ccggccaaaa
    10981 ccacgtccag cccgagggtg acgacgagcg gtgcgccata aaggaagaga tgccggagca
    11041 cggccggatc gaagacgggt gtggcaactt ggcgccacgc cgaccacaag gacccgagct
    11101 cgcgccgtgt gggggcgacg accgcggcta cggcaccggc ggcgagaccg aggagaatgc
    11161 cttcggcgcc ggcgccggcc accaccaggg cactgccgac cgcgagtgcc acgagggcac
    11221 gggtgatcgc catgcggccg tagacggcgg gcgtcagctg cgcgttggcg aggcgcagat
    11281 gaagctcggc gaagccctcg acgcagaaca gcaggaagcc caacggaagg agacggtcga
    11341 gcagcggatc gctcgtgagc cagagcgcca gcagggaggc gaggccggcg agcgccagca
    11401 tcgccaggaa ggcgatcagg atagtgccga gaaacgtgcc gcgacggacc tcgtaggccg
    11461 cgaggaagcg caacagcccg aggtccagcc agctcaggaa cgcggtgtgg gcaatgccga
    11521 gtccggcgag gaccatcgca tagatgccga actgctcggg cgccagaagc cgagtgtaga
    11581 tggcgagcgc ggcgaagttg agcaaggccg gcaagccgcg ggcggcgaga taggcgaggc
    11641 catgccgaag catcatggta cccttgcccc cgcccgacgg gcggtcgtca gcaaaccgat
    11701 gttctgatat ttcaagggtc cgctgctgcg tccttccttt cggagcccgt catgccgagc
    11761 cgcatcgacc cgtccgtgcc ggctgccggc cggcccgtca gcaaggccga tctgcggcgc
    11821 aatttcgccg ccgcccaggg tgagctggag catctgggct ggctgcgcca gccttacgcc
    11881 ggtgccgtcg agcggaccgg ggcggcgaag ctcgccgaaa ccctttcggc tgcggatttc
    11941 ggcgccatcg gcgacggcag cacggacgac acggcggcgc tgcaggccgc cctcgacgcg
    12001 ctgaagcgcc agggcggtgt cctcgccctg acgccgggcg cggtctaccg gatcagccgc
    12061 ggcctgctgc tcgaggacgc tgcgggcttt cgcatcgagg gtcgcgacgc gcgcctggtg
    12121 atggccgacg gggcaccggt cgccgtgcac tactggctgc tgcagctcgg ccgctgccgc
    12181 cgcttcacga tcgccgacct ccgcctcgac ggcaaccggc agacccgggc catcgggccc
    12241 gagccctggg ccaacaacct gcagctgcac gactgcagcg acttcgtcct gcagtcggtc
    12301 gagtgcagca atgcggtggt cgacggcttc atcctctacc atggccgccg cgacgcgccg
    12361 gcgaccttct gccggcgcgg gctcatgctg ggctgccgag cggtgaacgc cgcccggcag
    12421 ggcctgacca tcctgaacgg ccacgacatc cgcgtcgtcg cctgcgactt catcggcagc
    12481 aacggggtgc cgcccgaggc cggcatcgac atcgagagcc atatcgattc cgccgcaccg
    12541 agcaatgccc gggtgctgat ccagggttgc catttcgagg acaatcgggg gggcggcatc
    12601 ctgctcacgg ccttgggcgg ggcgcatggt ttcagcgtcg tcgattgcag cttcgcgagc
    12661 tgcgggcctt atggagtcga catcgtggca cccgactgca ccgtgcgcgg gtgccggttc
    12721 acccgcttcg gcccggaagt gcggcatggc gtggtcaaca tccgcacgcg cgaaggcggg
    12781 ctgccggcgc tggtcagcgg caacagcttc gatgacatcc tgatcggcga tgccaaccgg
    12841 gcggccatct acgttcggcc cgatgcctcg cccggcacgc tggtcatcga caaccggctg
    12901 aacgctgtcg acgccagcgg catcatcgcg gcagcgcccg gcacgatcgt ccgcggcaac
    12961 gtgatcgaag gcaatcagct cgaaggcatc cgcgtcggcc acgccgcact cgtcgagggc
    13021 aacagaatcg ccctgatgcg cccgggccgc ccggcgattc tcgtccatgg cggccgcacc
    13081 gtggtgcggg gcaaccacct gatcgattgc gacaacggca tggaaggcac gatcctggtg
    13141 ctcgacgacg gcacgggcga cagccgtccg ccggtgctcg acggcaacta tctcagcctg
    13201 tccggcggcg acgggcgaag cgtgcccgtg cggctgcgcg cggcggcgat cgaagccggc
    13261 acgaccgctt tcggctacgg ctgagcgtcg gacgctcacc cgtcggctcc cggcaccgca
    13321 cggacgccac gtcgcgcgat gcattccgca tagagggaac gatactcggc ggcgatggca
    13381 gtcatgctat agtgtgaaag aatccgctcg cgccccagtc gaccgagccg gacacgttcg
    13441 ccggccgatg tccggaccag cgtcgccagg gcatcggcca gggccgccgg gtcgcgcact
    13501 ggcaccacct gcccggccgc gccgatgacg cggcggcagt cgccgctgtc ggtggcgacg
    13561 cagggcacgc ccgaagccat cgcctcgccg atgaagttcg gaaagccctc gccccacgcc
    13621 gaggcgagga cggcgatgtc gaagcctgcg atcaccggcg ctgcatcgag cgtgggaccc
    13681 agcccgtgca ccctgccgcc cagcccgttg gccgcgacat ggtggacgag tgtcggcgag
    13741 tcggctgccg tgccggcgcc gagcagcacg aggtcgacgt cgagcccaaa atcgacgagg
    13801 cggcgcatgg cggcgaggag cgtggcgtgg tctttcatcg gccgccactc cgccgcgatg
    13861 ccgatgagca cgctgttggg accgaggccg agccgtgccc gcaggttccc tttggcctcg
    13921 ggatcgggcg cgaatcgatc cggatcgaag ccgttgccga tgacctgccg gcgttgcgct
    13981 cgatagccga tggcctcgtg ctggcgggcg gccacctcgg cattgtagac gatggcttcc
    14041 ggtcggccgg aaagccgggc gccgagccgc acgatctgcc gcgtgagcca gcgctcgcgt
    14101 tccggcgcgt ccagggcacc cctgatgttc cagagaaggg ccgcccgggc gcatcgtcgt
    14161 tcaaggccgc gcgccagggc caggccgacg ctggccgcga gattgccgtg atacatccag
    14221 ccctgaacga ggtcgggctg aagctggcgc agcagactgg cgaggcggac ggcagccatg
    14281 ggcgagaagc cgccacgcgc gaggtcgagg ctgtgcagcc gggcggtggc cgcgagcgcc
    14341 gggcccagcg cgccggccgg catgagcgtg accacatgcg tcgtcgtggc cgcgctcacc
    14401 ccggcctcga ccagcttcag cagcatgcgc tgggcgccgc cgagctcgag attggttgcg
    14461 acatgcacca ccgtggcggc gagcggcgcc ggtcgtgggc ccgtgatcgg gagggcacgg
    14521 gcaggcgttc ctgtcgcatg gacgggtgga gccgtgggca attcttccat gccccggatc
    14581 acggtcgggc gcaccctcgg atcacctcgc agatgcggtc gaggtcgccg tctccgagtg
    14641 cggagcccga aggcaggcag agaccctggt cgaacaaagc tttgccgacg ccgccgccga
    14701 agcaggtggc accggcgaac accggctgca tgtgcatcgg tcgccagagc ggccgggcct
    14761 cgatgtcctg cgcctcgagc gccagccgca ccgcctcgcg gtcggcgccg aacgcgcggg
    14821 gctcgatcag gatgcaggtc agccagcgat tggcccggcc gtagccggcc tcgggcatga
    14881 aggtgatgcc atcgagccgt cccaggcgct gctggtagcc ggagaaaatg gcgcggcgct
    14941 gccggacgcg atcgtcgagc acggccagct gcccgacgcc gatcgccgcc agcacattgc
    15001 tcaaccggta gttatagccc agctcgacgt gctcgtaatg cggcaccggc tggcgtgcct
    15061 gctgggagag gccgcgcgcg tgctcgatca gcgccgcgtc gtctgaggcg agcatcccgc
    15121 cgccgcctgc ggtgacgatc ttgttgccgt tgaacgagta gagggcggcc ctggcgccct
    15181 tgccggcatg ccggccccgg taggtggcgc ccagcgcctc ggcggaatcg gcgatcagcg
    15241 ggatctcgta ggcgtccgtc accgcgcgca gtgcatccag gtcgcaggcc tggccataga
    15301 ggtcgcaggg gaccaccgcc ttcggcagcc ggccctgccg cgcgccgccc gccagcgctt
    15361 cggcgagcag gcccggattc atgcaccagg tttcgggatc ggcgtcgacg aagatcggct
    15421 ccgcgccgag ctgcccgatc ggtgccacgc cgccgatgaa ggtgaggtcg gcggcaatca
    15481 cgcggtcgcc cgggccgaca ccgagatggc gcagcgccag atgcagggca gcggtgccgc
    15541 tgaccaccgc gagacaatgg tgaaagcctg tcagcgccgc catcgcggcc tcgaagcggg
    15601 agagcatcgg cccggccggg gcaacatagt tggtggcgaa gacttcgtgc agcagcgcca
    15661 gctcgtcgcc tgccatgtgc ggcggcgaga ggaagacgcg gccgggcgtc gtcgtgcggg
    15721 tccggtccgg ggacgggtcg agcacgccct ggtcggcggc tgcggtcatg gcgatggtcc
    15781 gat
//
LOCUS       DAOTFI010000112        15114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4740_length_15114_cov_6.649113, whole genome shotgun sequence.
ACCESSION   DAOTFI010000112 DAOTFI010000000
VERSION     DAOTFI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 15114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15114
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4740_length_15114_cov_6.649113"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /gene="gmk"
                     /locus_tag="P5558_12640"
     CDS             <1..563
                     /gene="gmk"
                     /locus_tag="P5558_12640"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRY25205.1"
                     /translation="RAVLAQESELDLSISVTTRPRRPAEVDGRDYHFITPDRFAAMIE
                     AGELLEHATVYGHSYATPKAPVLAALAAGRDVLFDIDWQGAQQLREAVGEDMVGVFIL
                     PPSTEELAARLRTRAQDSDAEVQFRLAQIANDVTHWPEYDYVLINQDFENSVGAVRAI
                     IAAERLRRVRQPGLGEFVKQFRHQPS"
     gene            complement(535..1425)
                     /gene="rsmA"
                     /locus_tag="P5558_12645"
     CDS             complement(535..1425)
                     /gene="rsmA"
                     /locus_tag="P5558_12645"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRY25206.1"
                     /translation="MLPRASTPLAGLPRLADVIKNAGLVPDKRLGQHFLLDPAILGRI
                     AAAPGKLEGRPVLEIGPGPGGLSRALLDRGARLWAIERDRRCTAALAPLVEAAAGRLV
                     LIEADALDVDIAELARAHAVDQPWAIVANLPYNIGTELVLRWLHRLDCVASLHVMLQK
                     EVALRLAAEPGSGDYGRLALVAQSLCRVRRCFDIPAGAFQPPPRVTSSLVELIPLPAT
                     GRPSGDRLRAIEQIGRVAFGQRRKMLRASLKGLVPDPEPLLAAAGIAADRRPETLGLA
                     EFDRLAAAWQTLRKAGGGTA"
     gene            complement(1443..2747)
                     /locus_tag="P5558_12650"
     CDS             complement(1443..2747)
                     /locus_tag="P5558_12650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY25207.1"
                     /translation="MPSRTLLALLLAALLGLAAPPAAMAQDQAPQQQPAAPQSVQRIA
                     AVVNDDIVSTQELRDRLALTLLFSNLPADPETQQRVAPQVLRRVIDEQLQLQEARRRN
                     IRVPRQEVDGALANAAAANNMSVNQLFAFLTEQGIDPQALRRQIEAQLTWFRVVREIF
                     AENVVVTDQQVELALAANQQGGQAELLLSEIVLPIYDPARANEVLAEAEELRAAIRQG
                     GDFAAIAQQVSASPSGAEGGDLGWVRVDSVQTALRPLLSGLGAGQMSDPIATPAGVQF
                     FLVRDRRITTGAVAPDVRELAQLLFPLAPNATEAETAEVLARARAAAEQIETCGDIER
                     LARQLALPNSGNIGWLRAADLPVDMAQTIATLPVGTLSRPVRGVNGIHLLMSCKDGVS
                     SEDEARRALLRRTLEEEQIQKLAARYLRDLRQEAFIDVRIGN"
     gene            complement(2797..4992)
                     /gene="lptD"
                     /locus_tag="P5558_12655"
     CDS             complement(2797..4992)
                     /gene="lptD"
                     /locus_tag="P5558_12655"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HRY25208.1"
                     /translation="MVEWRVPWFLLALVFWMFARVDSALARPAGLDTTPIVLNADLIS
                     YSTAEGRVTAIGNVELHQDGRTLLTDRIAYDIEAGTVAATGNIILIEASGDAVFADEL
                     MLDDRLTAGFVDGIGVLLEDGSRIAAVKGVRSDGRTTTLDRAVYSPCEVCAESDEPLW
                     QIKAERVVHDQATGTIAYRNARLEVAGVPVLYTPYFYHPDPSVERRTGFLVPSFGSDS
                     ELGFTLETPFFIDLAPNRDLTLVPLVTSQAGVFLAGEYRDLQTFGLTELGGGITYTNP
                     YQRRNADGQTEPASGNEVRGFVEGRGRYVVSERDRAGFDLNLASDNTVLERYNISNAD
                     VLQNHAYVERFDGRDYLAANTYGFQTLRENDDQDEIPIVLPLAVSKLYSGRLIWGSQA
                     DWSSNVLGLTRDEGLDTRRLSSEIGWHLPGIGRFGDVWSLRLSTRGDVYNTDGDPQTG
                     SGDGGVNTAGRLVPTANLDWGLPLVGTTGSWSHLIEPRVSFNYTPGNLNDSDIPNEDS
                     QVFEFDETNLFKPSRFTGIDRVESGARVSYGLGFNSLGPEAWRLSGLVGQSLRSGPDG
                     LYPQGSGLEDPLSDFVGRFDVRPSELLDLGYRFRADKTTLALRRSDLTVAFGPPRLRF
                     DIQYLRLTEEVEEIASEDLNSREEIVAGFRFQVFDSLAIGARTRRDLKEDRTVTNQYG
                     LVYTNPCLVLVAGLEQNFTQKGELDDEVTFTVRISLRSLGDLDAGTDIF"
     gene            5091..6584
                     /locus_tag="P5558_12660"
     CDS             5091..6584
                     /locus_tag="P5558_12660"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRY25209.1"
                     /translation="MELNFVEGEWPASGTVVVGITAPSALGAAATSLDKKSKGLIRKA
                     LERAGGLKHGKFLELVLPRGLDLDRLLLVGIGKPEGLSRLDREELGASIARKLEALAV
                     EDAALAPLDGLELGADAAAAAADLALGAKLASYAFRKYQTPDKEEPKRLGRLTVHLAA
                     APDADRPALVEAQAAGVALARDLVSEPANVLYPESFAERCRELEELGVEVEVLGEAEL
                     EKLGMRALLGVAQGSVRQPRVVVMRWRGAEQAEPLVLVGKGVCFDTGGISIKPAGGME
                     EMKWDMAGAGAVVGAMHAIAGRQARAHVVGIIGLVENMPSGTAQRPGDVVEAMSGKTI
                     EIINTDAEGRLVLADVLHYAKERLDPAAMVDLATLTGAVVVALGHENAGLFANDEKLA
                     GNLARAAEAVGEGLWRLPLGKAYAKHIKSQIADIKNVGRGREAGATAGAVFLEEFVGG
                     LPWAHLDIAGVAWSSREQPLSAKGATGFGVRLLDELVRLAYEADAKG"
     gene            6581..7036
                     /locus_tag="P5558_12665"
     CDS             6581..7036
                     /locus_tag="P5558_12665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRY25210.1"
                     /translation="MTELGFYHLTRSGLDAALPRLLEKIRSAGHRVVVRLADAERLDQ
                     LDRLLWTFRNDSFLPHGTRQDGHGQAQPIWLTTGTDTPNGATVLVLIDGAEAADAASF
                     ERVLELFDGRDAEAVARARERWRWAETQGMDRVYWQQDERGRWQRAAAS"
     gene            7105..7527
                     /gene="ndk"
                     /locus_tag="P5558_12670"
     CDS             7105..7527
                     /gene="ndk"
                     /locus_tag="P5558_12670"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRY25211.1"
                     /translation="MAVERTLSIIKPDATRRNLTGAINQRFEEAGLRIVAQKRLQLTR
                     GQAEAFYAVHKERPFFAGLVAFMTSGPVVAQVLEGEDAVARNRQVMGATDPAKADAGT
                     IRKDFAENIEANSVHGSDSAENAAIEIRFFFSDIEIVG"
     gene            7629..7985
                     /locus_tag="P5558_12675"
     CDS             7629..7985
                     /locus_tag="P5558_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRY25212.1"
                     /translation="MTHLGPFEARPDDVRAADALVLLTTENLGYMSGALKDFFDRSYY
                     PCLDATDALPYALLVRAGQDGTGTCRAVASITGGMRWKAVHEPVVCRGQWDDTFLDRA
                     TDLGTFMAAGLEAGIF"
     gene            complement(7995..9488)
                     /locus_tag="P5558_12680"
     CDS             complement(7995..9488)
                     /locus_tag="P5558_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRY25213.1"
                     /translation="MPDTVLIVLRLVALAVLAGVAWPALRMAASTAFRNRLGAHPGLP
                     HLILAAIVGYAGVVLALAMAPLPLLAVAAAAALVVAGLAHWRARPDFGRRRGLPPGSL
                     AATSLALVEDHRHLLDQARLHGPVFKIGRFGQKIVCVQGLERCNALLRRHGDDLVSGG
                     LPFDRLIPGGFIRYMPPDRHARYRRLLAGALTPELVRAGRPLMAEEAALALAAMAGQS
                     TATGIDPAPHLLRYTTRSMLRLFLGLGRDDPMLGKALALYPVIASANLSRRTRKRVSA
                     ALATLNGLFAEAIRRSASGSSPSLAAGLVAARTDAPDDPTLLGNLVYMIETGGRDTAD
                     LLLWVLKELADHPDQAAELGRTADPGLARRIVLETLRREQSEYVVRRVMRPITVEGYT
                     VPAGWLLRFCVRESHRDPAVFADPERFDPDRFLGDPPRANAYAPFGLGVHTCLGRSLV
                     LALATDFLTPLGGGHDWRVTADGPRVFGSFHWMPSRRFRLAFSSRAA"
     gene            complement(9470..10183)
                     /gene="purN"
                     /locus_tag="P5558_12685"
     CDS             complement(9470..10183)
                     /gene="purN"
                     /locus_tag="P5558_12685"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRY25214.1"
                     /translation="MGAARVAVLISGSGSNLEALIRATRSGAAAGEIVLVVSNRADAF
                     GLERARRHGIATAVIPHRDHPSRAAFEAALHAALVDAAIDIVCLAGFMRILEPGFVEQ
                     WHDRMLNIHPSLLPALPGLRTHARALAAGALVHGCTVHLVRPTLDVGPILVQGVVPVL
                     PGDDAATLAARVVGLEHTCYPLALDLVAGGTAVVDGEIVTAPPGHRRLVLHELFETNG
                     QAAGPIRSTAPDRPCPTPS"
     gene            complement(10156..11247)
                     /gene="purM"
                     /locus_tag="P5558_12690"
     CDS             complement(10156..11247)
                     /gene="purM"
                     /locus_tag="P5558_12690"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRY25215.1"
                     /translation="MTQEEQGGDAYARAGVDIAAGNELVRRIAPLAAATARPGTMAAL
                     GGFGAVFDLAAADFRDPLLVAATDGVGTKLLIAEAMGRHDTVGIDLVAMCVNDLVVQG
                     AEPLFFLDYLATGRLELEAATGLIAGIAKGCALAGCALIGGETAEMPGHYPPGRFDLA
                     GFALGAVERAELLPKPVAAGDVVLGLASSGLHSNGFSLVRRIVADAGLDLDARPPFST
                     DQTLGEVLLEPTRIYVRSCRAALRHGGVKALAHITGGGLIENPPRVVPDGLTIRLDAH
                     AWHLPAVMRWLAGAGELEPGSLGRTFNCGLGMLLFVEPAKADELLALLEGQGETVWRV
                     GGIEAGVPGPARVVIDGIETAWAPPASPS"
     gene            11350..12444
                     /locus_tag="P5558_12695"
     CDS             11350..12444
                     /locus_tag="P5558_12695"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HRY25216.1"
                     /translation="MALAKRPLALPGLLLATIAAVAGAALADPYTVNGIAVDVTAENA
                     VLARETAVTEAQVTGLRELLERLTLPSHYSRLPVVGSETATSLVTSYQVEEENVAATR
                     YVAEMAVTYDAEGVQNILEGTGVPIVVDLPPPLLVVPALNADGGLAVFDGVSAWRDAW
                     ARESGRNTLLDIVLPLGDLTDLRTLGGQALAANPQTALDEAALRYGTDGAVLVTATAD
                     SIEAPTRIDLQLGGAAGWPTTVSQLTVAMEGEPEEVWAAAARRVMGELEAEWKRENLV
                     AFDSLAQLEVAAPLGGLEDWAAIRSGLEEVSAIRRLDVMSFSQAEAELVLNYIGNVDQ
                     LQRALAARGLGLSQGAERWRLQRTAGRNAG"
     gene            12405..13034
                     /locus_tag="P5558_12700"
     CDS             12405..13034
                     /locus_tag="P5558_12700"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY25217.1"
                     /translation="MAAAANGWPERGLNAVRRLLAGAAGAVRRQLANAITLARLVAVL
                     PIWMLIEDGRHEAALLLFSVAAVSDAVDGAVAKLLRTASAFGAVLDPLVDKLMLGCLF
                     IIAGTLGWTSLWLVALVVGRDMALAVGVVRLELLAHSRRLEPLLIGKLCTMTQFFFFG
                     VLLAETGGLVPFGILHYLAASAVVLTALASLAAYLWFARAGAHAKAAGV"
     gene            13059..13808
                     /locus_tag="P5558_12705"
     CDS             13059..13808
                     /locus_tag="P5558_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRY25218.1"
                     /translation="MLTTLAANQLVLDLPTDDADRLEDFLRSPANEAALDAVLHWPAW
                     PAPALIVWGPAGSGRTHLARIWARRTGARLLDGSRLWAERPPYGLVGECRTCVVDDAD
                     RVRDDRALLHVYNLVAGQGGHLLLTASRPLADWGLELPDLVSRLRTAWSCRIDEPDDP
                     LLAALLVKQLQDRQLAVSPGVIAYLSRHMERSFAAARRLVRELDRASLRARRPITMGL
                     ARAVLATLDEDEIEDRPEPARADMLNYPGAF"
     gene            13811..14965
                     /locus_tag="P5558_12710"
     CDS             13811..14965
                     /locus_tag="P5558_12710"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRY25219.1"
                     /translation="MNALTRLQDLAARPTLMVAGLMTGTSVDGLDVALCRIRPALDTM
                     PELVLGRTLKLPADLRSALLAAKDAGVPALARADLALGRFQAEALAGLLAESGLGPVD
                     LVGSHGQTAYHEHRVTTLQIGEPACLAQALDCPVVADFRRADVAAGGSGAPLVPIFDR
                     LFLGQPGKGVLALNIGGIANFTALPAGGGEPVAMDCGPGNMIVDGLVRRMTGGKQDYD
                     ADGAFAANRRIDRNLLDELASMPALHTPPPRSLGREEFGDAFVDELVARVAPRSEAEW
                     CELIATATALSAEALARTVRAHVERTTKVDRVLVSGGGVHNATLMRQIELRLPGTEVA
                     STASVGLDPDFKEAIAFAAFAVLTLFAPPVATGGFTGADRPVRLGKLILP"
BASE COUNT         2177 a         5276 c         5597 g         2064 t
ORIGIN      
        1 cgcgggcggt gctggcgcag gaaagcgagc tcgacctctc catctcggtg accacccggc
       61 cgcggcggcc ggccgaggtg gatggccgcg actaccattt catcacgccc gaccgcttcg
      121 ccgccatgat cgaggcgggc gagctgctgg agcacgcgac ggtctacggc cattcctacg
      181 ccacgccgaa ggcgcccgtg ctggccgccc tcgccgccgg ccgcgacgtg ctcttcgaca
      241 tcgactggca gggtgcccag cagctccgcg aggcggtcgg cgaggacatg gtcggcgtgt
      301 tcatcctgcc accctcgacc gaggagctgg cggcccggct ccgcacgcgt gcccaggaca
      361 gcgacgccga ggtgcagttc cgcctggccc agatcgccaa cgacgtgacc cactggccgg
      421 agtacgacta cgtcctgatc aaccaggatt tcgagaacag cgtcggcgcg gtccgggcga
      481 tcatcgccgc ggaacggctg agacgggtgc gccagccggg cctcggcgag ttcgtcaagc
      541 agttccgcca ccagccttcc tgagggtctg ccaggcggcg gccaggcggt cgaactcggc
      601 gagacccagg gtttccgggc ggcggtcggc ggcgatgccg gcggcggcga gcaggggctc
      661 ggggtcgggc accagcccct tgaggctggc acgcagcatc ttgcgccgct ggccgaaggc
      721 caccctgccg atctgctcga tggcgcgcag acggtctccc gagggtcgcc cggtggccgg
      781 gaggggaatg agctcgacca ggctggaggt gaccctgggc ggcggctgga aggcgccggc
      841 cgggatgtcg aagcagcgcc gcacccggca gagcgattgg gcgacgaggg cgaggcggcc
      901 atagtcgccg ctgccgggct cggccgcgag gcggagcgct acctccttct gcagcatgac
      961 atgcaggctg gccacgcaat cgagccggtg cagccagcgc agcaccagct ccgtgccgat
     1021 attgtagggc aggttggcga cgatcgccca gggctggtcg acggcgtgcg cgcgggcgag
     1081 ctccgcgatg tcgacgtcga gcgcgtcggc ctcgatcagc accagccgcc cggcggccgc
     1141 ctcgaccagg ggggcgagcg ccgccgtgca gcggcggtcg cgctcgatcg cccagagccg
     1201 cgcgccccgg tcgaggagcg cgcggctgag gccgcccggg cccgggccga tctcgagcac
     1261 cggccggcct tcgagcttgc ccggtgccgc ggcgatccgc ccgaggatcg ccggatcgag
     1321 gaggaagtgc tggccgaggc gcttgtcggg gacgaggccg gcgttcttga tgacgtcggc
     1381 gaggcgcggc agcccggcga ggggcgtcga cgcccttggg agcacccctg gggacggcgc
     1441 cgtcagttgc cgatccggac gtcgatgaag gcctcctggc ggaggtcgcg caggtagcgg
     1501 gccgccagct tctggatctg ctcctcctcg agcgtgcggc ggagcagggc ccgccgcgcc
     1561 tcgtcctccg agctgacgcc gtccttgcag gacatcagga gatggatgcc gttgacgccg
     1621 cgcaccgggc ggctgagcgt gccgacgggc agcgtggcga tcgtctgcgc catgtcgacc
     1681 ggcaggtcgg cggcgcgcag ccagccgatg ttgccggaat tgggcaaggc gagctggcgc
     1741 gccagccgct cgatgtcgcc gcaggtctcg atctgctcgg ccgcggcccg ggcccgggcg
     1801 aggacctccg cggtctccgc ctcggtcgcg ttgggggcca gcggaaagag caattgggcc
     1861 agctcgcgca catccggcgc caccgcaccg gtggtgatcc ggcgatcgcg aaccaggaag
     1921 aactgcacgc cggccggggt ggcgatcggg tcggacatct gcccggcgcc gaggccggag
     1981 agcagcgggc gcagcgccgt ctgcaccgaa tcgacgcgga cccagccgag gtcgccgccc
     2041 tcggcgccgc tcggcgaggc cgagacctgc tgcgcgatgg cggcgaaatc gccgccctgc
     2101 cggatggctg cgcgaagctc ctcggcctcg gccagcacct cgttggcgcg ggccgggtcg
     2161 tagatcggca gcacgatctc cgacagcagc agctccgcct gcccgccctg ctggttcgcc
     2221 gcgagcgcca gctccacctg ctggtcggtg accaccacgt tctccgcgaa gatctcgcgg
     2281 accactcgaa accaggtgag ctgggcctcg atctggcggc gcagcgcctg cgggtcgatg
     2341 ccctgctcgg tgaggaacgc aaagagctgg ttgaccgaca tgttgttggc ggcggcggca
     2401 ttggcgagcg cgccgtcgac ctcctgccgc ggcacccgga tgttgcgccg gcgcgcctcc
     2461 tggagctgca gctgctcgtc gatcacgcgg cgcaggacct ggggcgccac acgctgctgg
     2521 gtctccgggt cggccggcag gttcgagaag agcagggtca gcgccaggcg gtcgcgcagc
     2581 tcctgggtcg agacgatgtc gtcattgacc acggcggcga tgcgctggac cgactggggg
     2641 gccgccggct gttgttgcgg cgcctggtcc tgcgccattg ccgccggcgg tgcggcgagg
     2701 ccgagcaggg cggcgaggag gagggcgagg agggttcggc ttggcatgga tgtgtccagg
     2761 gggaggccgg gccggcggtg ggtgccgcgg cgacggtcag aagatgtccg tgccggcatc
     2821 gagatcaccg aggctgcgca gcgagattcg aacggtgaac gtcacctcgt catcgagctc
     2881 gcccttctgc gtgaagttct gctcgagccc ggcgaccagc acgaggcagg ggttcgtata
     2941 gaccagtccg tactggttcg tcaccgtgcg atcctccttg aggtcgcgcc gcgtgcgcgc
     3001 gccgatggcg aggctgtcga acacctggaa gcggaagccg gcgacgatct cctcgcggct
     3061 gttgagatcc tccgacgcga tctcctcgac ctcctcggtc aggcgcagat actggatgtc
     3121 gaagcgcagc cgcggcgggc cgaaggcgac cgtgaggtcg ctgcggcgca gcgccagggt
     3181 cgtcttgtcg gcgcgaaagc gatagcccag atcgagcagc tccgagggcc gcacgtcgaa
     3241 gcggccgacg aaatcggaga gggggtcctc gaggcccgag ccctgcgggt agaggccgtc
     3301 ggggccgctg cgcaggctct gcccgacgag gccgctgagc cgccacgcct ccggcccgag
     3361 cgagttgaag ccgaggccgt aggagacccg cgccccgctc tcgacccggt cgatgccggt
     3421 gaagcgcgac ggcttgaaga gattggtctc gtcgaactcg aagacctggc tgtcctcgtt
     3481 ggggatgtcg gaatcgttga ggttgcccgg ggtgtagttg aaggagaccc ggggctcgat
     3541 cagatgcgac cagctgccgg tcgtgccgac cagcggcaga ccccagtcga gattggcggt
     3601 cggcaccagc cggcccgcgg tgttgacacc accgtcgccg gagccggtct gggggtcgcc
     3661 gtcggtgttg tagacgtcgc cgcgggtcga gaggcgcagc gaccagacat cgccgaaccg
     3721 gccgatgccc ggcagatgcc agccgatctc gctcgagagg cggcgggtgt cgaggccctc
     3781 gtcgcgggtc aggccgagga cgttggagct ccagtcggcc tgcgagcccc agatcagccg
     3841 gccgctgtag agcttgctca ccgccagtgg caggacgatg gggatctcgt cctggtcgtc
     3901 gttctcgcgc agcgtctgga agccgtaggt gttcgccgcg aggtagtcgc gcccgtcgaa
     3961 ccgctcgaca taggcgtggt tctgcagcac gtcggcattg gagatgttgt agcgctcgag
     4021 aacggtgttg tcgctcgcga ggttgaggtc gaagccggcg cggtcgcgct cgctgaccac
     4081 gtagcggccg cgcccctcga cgaagccgcg cacctcgttg ccgctggccg gctcggtctg
     4141 gccgtccgcg ttgcggcgct gataggggtt ggtgtaggtg atgccgccgc cgagctcggt
     4201 cagcccgaag gtctgcaggt cccggtactc gccggccagg aagacgcccg cctggctggt
     4261 gacgagcggg accaaagtga ggtcgcgatt gggggcgagg tcgatgaaga acggcgtctc
     4321 gagggtgaac ccgagctcgc tgtcgctgcc gaacgagggc accaggaagc cggtgcggcg
     4381 ttcgaccgag ggatcggggt ggtagaagta aggcgtataa agcactggaa caccagcaac
     4441 ttccagcctc gcattgcggt acgcgatggt tccggtggcc tggtcgtgga cgacgcgctc
     4501 ggccttgatc tgccagagcg gctcgtcgct ctcggcgcac acctcgcatg ggctgtagac
     4561 cgcgcggtcg agcgtcgtcg tccggccgtc gctgcgcacc cccttgaccg cggcgatgcg
     4621 cgaaccgtcc tcgagcagca cgccgatgcc gtcgacgaag ccggccgtga ggcggtcgtc
     4681 gagcatcagc tcgtcggcga acaccgcatc cccggacgcc tcgatcagga tgatgttgcc
     4741 cgtcgccgcg accgtgccgg cctcgatgtc atacgcgatc cggtcggtga gcagcgtgcg
     4801 gccgtcctgg tgcagctcga cattgccgat ggcggtgacc cggccttcag cggtcgagta
     4861 gctgatcaga tcggcgttga gcacgatcgg cgtggtgtcg agcccggccg gccgggccag
     4921 ggccgaatcg acacgcgcga acatccagaa aaccagggcg agcaggaacc agggcactcg
     4981 ccattcaacc atgacgtcca acctgcacgc ttgtgctcac cgccctgcca agccatctga
     5041 tcccccgggc tgctaccaca ccgccgcggg catgaggaga gaaaccgttg atcgaattga
     5101 acttcgttga aggcgagtgg ccggcgagcg gcaccgtggt cgtgggcatc accgccccct
     5161 cggccctcgg cgctgcggca acgtccctcg acaagaagag caagggcctg atcaggaagg
     5221 cgctcgagcg ggccggcggg ttgaagcacg gcaagttcct ggagctggtg ctgccgcgcg
     5281 ggctcgacct ggaccggctg ctgctggtcg ggatcggcaa gccggaaggg ctcagccggc
     5341 tggatcgcga ggagctcggc gccagcatcg cgcgcaagct ggaggcgctg gccgtcgagg
     5401 atgcggcgct cgccccgctg gacgggctgg agctcggcgc cgatgcggcc gcggccgcgg
     5461 ccgatctggc gctcggcgcc aagctcgcga gctacgcctt ccgcaagtac cagacgccgg
     5521 acaaggagga gccgaagcgg ctcgggcggc tcaccgtcca ccttgccgca gcgcccgacg
     5581 ccgaccggcc ggcgctggtc gaggcgcagg cggccggcgt ggccctggcc cgcgacctcg
     5641 tctcggagcc ggccaacgtg ctctatcccg agagcttcgc cgaacgctgc agggagctgg
     5701 aggagctcgg ggtcgaggtc gaggtgctcg gcgaggccga gctcgagaag ctgggcatgc
     5761 gcgccctcct gggcgtggcg cagggcagcg tgcggcagcc ccgggtcgtc gtgatgcgct
     5821 ggcgcggtgc cgagcaggcg gagccgctgg tgctggtcgg caagggtgtg tgcttcgaca
     5881 cgggcgggat ctcgatcaag cctgccggcg gcatggagga gatgaagtgg gacatggcgg
     5941 gtgccggggc cgtggtcggc gccatgcacg ccatcgccgg ccgccaggcc agggcgcacg
     6001 tggtcggcat catcggcctg gtcgagaaca tgccctccgg caccgcccag cggccgggcg
     6061 acgtggtcga agccatgtcg ggcaagacca tcgagatcat caacaccgac gccgagggcc
     6121 ggctcgtgct ggccgacgtg ctgcactacg cgaaggagcg cctcgacccg gcggccatgg
     6181 tcgacctcgc gacgctcacc ggggcggtgg tcgtcgccct cggccacgag aatgccgggc
     6241 tcttcgccaa tgacgagaag ctcgccggca atctcgccag ggccgccgag gcggtcggcg
     6301 aggggctctg gcggctgccg ctcggcaagg cctacgccaa gcacatcaag tcgcagatcg
     6361 ccgacatcaa gaatgtcggc cgcgggcgcg aggccggggc gacggccggg gccgtcttcc
     6421 tcgaggagtt cgtgggcggg ctgccctggg cgcatctcga catcgccggc gtcgcctgga
     6481 gcagccgcga gcagccgctc tcggccaagg gggccacggg cttcggcgtg cgcctgctcg
     6541 acgagctggt gcggctcgcc tacgaggccg atgcgaaggg gtgaccgagc tcggcttcta
     6601 ccacctgacc cggtcgggcc tcgacgcggc cctgccgcgg ctgctcgaga agatccgctc
     6661 ggccggccat cgggtggtgg tgcgcctcgc cgacgcggaa cgtctggacc agctcgaccg
     6721 gctgctctgg acgttccgca acgactcctt cctgccgcac ggcacgcgcc aggacggcca
     6781 tggccaagcc cagccgatct ggctcacgac cggcaccgac accccgaacg gcgccacggt
     6841 gctcgtcctg atcgacggtg cggaggcggc ggacgcggcg agcttcgagc gggtgctcga
     6901 gctcttcgat ggccgcgacg ccgaggcggt ggcccgggcg cgcgagcgct ggcgctgggc
     6961 cgagacgcag ggcatggacc gcgtctattg gcagcaggac gaacgcggcc gctggcagcg
     7021 cgccgcggct tcctaactgc cggtgcggct gctagaagcc ggccgagcct tttccgacca
     7081 tccccagatt tcaggagttc tcccatggcc gtggagcgca cgctctcgat catcaagccc
     7141 gacgccaccc gccgcaacct gacgggggcc atcaaccagc gcttcgagga ggccgggctc
     7201 aggatcgtcg cccagaagcg gctgcagctc acccggggcc aggccgaggc gttctacgcg
     7261 gtccacaagg agcgcccgtt cttcgccggc ctcgtcgcct tcatgacctc gggcccggtg
     7321 gtggcgcagg tgctcgaggg cgaggatgcg gtggcgcgca accgccaggt gatgggcgcc
     7381 accgacccgg ccaaggccga tgccggcacc atccgcaagg acttcgccga gaacatcgag
     7441 gccaactcgg tgcacggctc ggacagcgcc gagaacgcgg cgatcgagat ccgcttcttc
     7501 ttcagcgaca tcgagatcgt cggctgaagc ggcttctcgt catcgcccac gcgccgtcgc
     7561 cgaacacggc ggcgctgcgc gacgcgctgc tgcggggcgc caccctgccg gagatcgaag
     7621 gtgtcgaggt gacccacctc ggccccttcg aggccaggcc cgacgatgtt cgggcggccg
     7681 atgccctggt gctgctgacg accgagaatc tcggctacat gagcggggcg ctcaaggact
     7741 ttttcgatcg ctcctactat ccctgcctcg atgccaccga cgcgctgccc tacgcgctcc
     7801 tcgtccgcgc cggccaggac ggcaccggca cctgccgggc ggtcgccagc atcacgggcg
     7861 gcatgcgctg gaaggccgtg cacgaacccg tggtctgccg cggccaatgg gacgacacct
     7921 tcctcgaccg cgccaccgac ctcggcacgt tcatggccgc cgggctggag gcgggcatct
     7981 tctgaggccc gcgctcaggc cgcgcgggaa ctgaacgcca ggcgaaagcg acggctcggc
     8041 atccagtgga agctgccgaa gacgcgcggc ccgtcggccg tgacccgcca gtcatggccg
     8101 ccgccgagcg gcgtcaggaa atcggtcgcc agggcaagga cgaggctgcg gccgaggcag
     8161 gtatggacgc cgagcccgaa gggcgcgtag gcattggcgc ggggcgggtc gccgaggaat
     8221 cggtcgggat cgaaccgctc gggatcggcg aagaccgcag gatcgcgatg gctctcgcga
     8281 acacagaagc ggagcagcca gccggccggg acggtgtagc cctcgaccgt gatcggccgc
     8341 atcacgcggc ggacgacata ctcgctctgc tcgcggcgca gcgtctcgag cacgatgcgc
     8401 cgggcgaggc ccggatcggc cgttcgcccg agctcagccg cctggtccgg atgatcggcg
     8461 agctccttca ggacccagag cagcaggtcg gcggtgtcgc ggccgccggt ctcgatcatg
     8521 tagacgagat tgccgagcag cgtcgggtca tccggcgcat ccgtccgggc ggcgacgagg
     8581 cctgccgcca gcgacggcga agagccgctc gccgaccgcc tgatcgcctc ggcgaacaga
     8641 ccgttcaacg tcgcaagcgc ggcgctcacc cgtttgcggg tccgccgcga aagattggcc
     8701 gaggcgatca ccggatagag ggcgagcgcc ttcccgagca tcgggtcgtc ccggccgagg
     8761 ccgagaaaga gcctgagcat gctcctcgtg gtgtagcgga gcagatgcgg tgcggggtcg
     8821 atgccggtgg cggtcgattg gccggccatg gcggcgagcg ccagcgccgc ctcctcggcc
     8881 atcagcggcc ggccggcgcg gaccagctcc ggcgtcagcg ccccggccag cagccggcga
     8941 tagcgggcat ggcggtcggg cggcatgtag cggatgaagc cgcccgggat cagccggtcg
     9001 aacggcaggc cgccggagac gagatcatcg ccatgccggc gcagcagggc gttgcagcgt
     9061 tccagcccct ggacgcagac gatcttctgg ccgaaccggc cgatcttgaa gaccgggccg
     9121 tgcaggcggg cctggtccaa aaggtgacga tggtcctcca ccaaggcgag cgaggtggcg
     9181 gcgagcgaac cgggcggcag gccgcgccgt cggccgaaat cgggtctggc acgccagtgg
     9241 gcgagacccg ccacgacgag cgctgccgca gcggcgacgg cgagcagcgg cagcggtgcc
     9301 atggcgagag cgaggacgac gccggcatag ccgacgatgg cggcgaggat caggtgcggc
     9361 aggcccggat gcgcgcccag ccggttgcgg aaggcggtcg atgcggccat ccggagcgcc
     9421 ggccaggcga cgccggccag cacggcgagc gccaccagtc tgaggacgat caggacggtg
     9481 tcgggcaagg gcggtccggc gcggtcgacc ggatcggccc ggccgcctgg ccattggtct
     9541 cgaaaagctc gtgcaggacc agccggcggt ggccgggcgg ggccgtgacg atctcgccat
     9601 cgacgaccgc cgtcccgccg gcgacgaggt cgagcgccag cgggtagcac gtgtgctcga
     9661 gcccgaccac gcgcgctgcg agcgtcgccg cgtcgtcgcc gggcagcacc ggcaccacgc
     9721 cctggaccag gatcggcccg acgtcgaggg tcggccggac gagatgcacg gtgcagccat
     9781 gcaccagggc accggccgcc agcgcccgcg cgtgggtccg caggccgggc aaagccggca
     9841 gcagggacgg gtggatgttc agcatccggt cgtgccactg ctcgacgaag cccggctcga
     9901 ggatgcgcat gaacccggcg aggcagacga tgtcgatggc ggcatcgacc agggccgcgt
     9961 gcagcgccgc ctcgaaggcg gcacggctgg gatggtcgcg gtgcggaatc acggccgtgg
    10021 cgatgccgtg ccgccgcgcg cgctcgaggc cgaaggcgtc ggcccggttg ctgacgacga
    10081 gcacgatctc gccggccgca gccccgctcc gggtcgcccg gatcagcgcc tcgagattgc
    10141 tgccgctgcc cgagatcagg acggcgacgc gggcggcgcc catgccgtct cgattccgtc
    10201 gatgaccacc cgtgccggac cgggcacccc ggcctcgatg ccgccgaccc gccacaccgt
    10261 ctcgccctgg ccctcgagca gggccagcag ctcgtcggcc ttggcgggct cgacgaagag
    10321 cagcatgccg aggccgcaat tgaaggttcg ccccaatgag ccgggctcca gctcgccggc
    10381 gcccgcgagc cagcgcatca ccgccggcag atgccaggcg tgcgcatcga ggcggatggt
    10441 gagtccgtcc ggcacgacgc gcggcgggtt ctcgatgagc ccgccgccgg tgatgtgggc
    10501 gagcgccttg accccgccat gccggagcgc tgcgcggcag ctccgcacgt agatgcgggt
    10561 cggctcgagc agcacctcgc ccagggtctg gtcggtggag aagggcgggc gggcgtcgag
    10621 gtcgaggccg gcatcggcga cgatccggcg gaccagcgag aagccgttgc tgtgcagccc
    10681 cgaggaggcg aggccgagga cgacgtcgcc ggcggcaacc ggcttcggca gcagctcggc
    10741 ccgctcgacg gcaccgagcg cgaagcccgc gaggtcgaac cggccgggcg ggtaatggcc
    10801 cggcatctcg gcggtctcgc caccgatcaa cgcacagccg gcgagggcgc agcccttggc
    10861 gatgccggcg ataagcccgg tcgcggcctc cagctcgagc cggccggtgg cgagataatc
    10921 caggaagaag agcggctccg caccctgcac gacgaggtcg ttgacgcaca tggcgacgag
    10981 atcgatgccg accgtgtcgt gccggcccat cgcctcggcg atcagcagct tggtgccgac
    11041 tccgtcggtg gcggcgacca ggagcgggtc gcggaaatcg gcggcagcca gatcgaagac
    11101 cgccccgaag ccgccgagcg cggccatcgt gccggggcgg gcggtggcgg ccgccagcgg
    11161 ggcgatgcgc cggaccagct cgttgccggc ggcgatgtcg accccggccc gggcataggc
    11221 gtcgccgccc tgttcctctt gtgtcaagct gcccgttcct cttgtatcga gcccagggcg
    11281 cgccctatct ctgcaaccac gcgcgatgcg ccggtcgtct ccatgcgcag gcggaactta
    11341 gggaagatca tggcacttgc aaagcgcccc ttggcgctcc ccgggctgct tctcgcgacc
    11401 atcgctgcgg ttgccggggc ggccctggcc gatccctaca cggtgaacgg gatcgccgtc
    11461 gacgtgaccg cggagaacgc ggtcctcgcc cgcgagaccg cggtgacgga agcccaggtg
    11521 acgggcctcc gggagctgct ggagcggctg accctgccga gccactacag ccgcctgccg
    11581 gtggtcggca gcgagaccgc cacctcgctc gtcacctcct accaggtgga ggaggagaac
    11641 gtggccgcca cgcgctacgt ggccgagatg gcggtgacct acgacgcaga gggcgtgcag
    11701 aacatcctcg aaggcacggg cgtgccgatc gtcgtcgacc tgccgccgcc gctcctcgtg
    11761 gtgccggccc tgaatgcgga tggcgggctt gcggtcttcg acggcgtctc ggcctggcgc
    11821 gacgcctggg cccgggagtc cggccgcaac acgctgctcg acatcgttct gccgctcggc
    11881 gacctcaccg acctgcgcac gctgggtggc caggcgctgg ccgcgaatcc gcagacggcg
    11941 ctcgacgagg cggccctgcg ctacggcacc gacggcgccg tgctcgtgac ggcgacggcc
    12001 gattcgatcg aggcacccac ccggatcgac ctgcagctcg gcggggccgc cggttggccg
    12061 acgacggtga gccagctcac ggtggccatg gaaggcgagc cggaagaggt ctgggcggcg
    12121 gcggcgcgcc gggtgatggg cgagctcgag gcggaatgga aaagggagaa cctcgtcgcc
    12181 ttcgacagcc tggcgcagct cgaggtggca gcaccgctcg gcgggctcga ggactgggcg
    12241 gcgatccgct ccggcctcga ggaagtctcg gccatccggc ggctcgacgt catgtcgttc
    12301 tcgcaggccg aggcggagct ggtcttgaac tatatcggca atgtcgacca gctgcagcgc
    12361 gccctggcgg cgcgcgggct gggcctgtcg cagggggcgg agcgatggcg gctgcagcga
    12421 acggctggcc ggaacgcggg ctgaacgccg ttcgccggct gctcgccggc gctgccggtg
    12481 ccgtccggcg gcagctcgcc aatgccatca ccctggcccg gctggtcgcg gtgctgccga
    12541 tctggatgct gatcgaggac ggcaggcacg aagcggccct cctgctcttc tcggtggcgg
    12601 cggtgagcga cgcggtcgac ggcgcggtgg cgaagctcct gcgcacggcc tcggccttcg
    12661 gtgccgtgct cgacccgctg gtcgacaagc tgatgctggg ctgcctcttc atcatcgccg
    12721 gcacgctggg ctggacctcg ctctggctcg tggcgctggt ggtcggccgc gacatggcgc
    12781 tcgcggtcgg cgtcgtccgg ctggagctcc tggcccacag ccgccggctc gagcccctgc
    12841 tgatcggcaa gctctgcacc atgacgcagt tcttcttctt cggggtgctg ctggccgaga
    12901 ccggcggact cgtacccttt ggcatcttgc actatctagc ggcctcggcc gtcgtgctga
    12961 cggcgctcgc ctcgctcgcc gcctacctct ggttcgcccg cgccggcgcc catgcgaagg
    13021 ccgccggtgt ctgaggccgt ctttatcttc ggatattcat gctgaccacg ctcgccgcca
    13081 accagctcgt cctcgacctg ccgaccgacg acgccgaccg gctcgaggac ttcctccgct
    13141 cgcccgccaa cgaggcggcg ctggacgccg tgctgcactg gccggcctgg ccggcgccgg
    13201 cgctcatcgt ctgggggccg gccggcagcg gccgcaccca tctggcgcgg atctgggcgc
    13261 ggcgcaccgg cgcccgcctg ctcgacggca gccgcctctg ggcggagcgc ccaccctacg
    13321 gcctcgtcgg cgagtgccgc acctgcgtcg tcgacgacgc cgaccgggtg cgcgacgacc
    13381 gggcgctcct gcacgtctac aacctggtgg ccgggcaggg cgggcatctg ctcctgaccg
    13441 ccagccgacc gctcgccgac tgggggctgg agctcccgga cctggtctcg cggctgcgca
    13501 cggcctggtc ctgccggatc gacgagccgg acgacccgct gctcgcggcc ctcctggtca
    13561 agcagctcca ggaccgccag ctcgccgtgt cgcccggtgt gatcgcctat ctcagccgcc
    13621 acatggagcg ctccttcgcc gcggcccggc ggctggtccg ggagctcgac cgcgcctcgc
    13681 tccgcgcccg ccggccgatc accatggggc tggcgcgggc cgtgctcgcg accctggacg
    13741 aggacgagat cgaggacagg cccgagccgg cccgggccga catgctcaac taccccgggg
    13801 ccttctgagc gtgaacgcgc tcacccggct gcaggacctg gccgccaggc cgacgctcat
    13861 ggtggcgggc ctcatgaccg gcacctcggt cgacgggctt gacgtggcgc tctgccgcat
    13921 tcgcccggcc ctcgacacca tgccggagct ggtgctgggc cgcaccctga agctgccggc
    13981 cgacctgcgc tccgcgctcc tcgccgcgaa ggacgccggc gtgccggcgc tcgcccgggc
    14041 cgacctggcg ctcggccgct tccaggcgga agcgctcgcg ggcctcctcg ccgagagcgg
    14101 gctcgggccg gtcgacctcg tcggcagcca cggccagacc gcctatcacg agcaccgggt
    14161 gaccaccctg cagatcggcg agcccgcctg cctcgcccag gcgctggact gccccgtcgt
    14221 ggcggatttc cgccgcgccg acgtggcggc gggcggcagc ggtgccccgc tcgtgccgat
    14281 cttcgaccgc ctcttcctcg gccagccggg caagggcgtg ctggcgctca atatcggcgg
    14341 catcgccaac ttcaccgcgc ttcctgccgg cggcggcgag cccgtcgcca tggattgcgg
    14401 ccccggcaac atgatcgtcg acggcctggt gcgccgcatg accggcggaa agcaggacta
    14461 cgacgccgac ggcgccttcg ccgccaaccg gcggatcgac cggaacctgc tcgacgagct
    14521 ggcatccatg cccgccctgc acacaccacc gccgcgctcg ctcggccgcg aggagttcgg
    14581 cgacgccttc gtcgacgagc tggtggcccg ggtggcaccc cggagcgagg ccgagtggtg
    14641 cgagctgatc gccacggcca cggccctttc cgccgaggcg ctggcgcgga ccgtccgagc
    14701 ccatgtcgag cgcacgacga aggtagaccg cgtgctggtg agcggcggcg gcgtccacaa
    14761 cgccacgctg atgcgccaga tcgagcttcg cctgcccggc acggaagtcg cctcgacggc
    14821 cagcgtcggg ctcgatccgg acttcaagga ggcgatcgcc ttcgccgcct tcgccgtgct
    14881 gacgcttttc gcgccgcccg tggcgaccgg cggcttcacc ggcgccgacc ggccggtgcg
    14941 gctgggcaag ctgatcctgc cctaggcccg caggcgatcg cccttggggt cgaagagcgg
    15001 cgcggcgagg cggcgggcgg ggcggcgctc tcccaggatc tcgacctcga actcgctcga
    15061 cgaggccagg gcggcaggga catagcccag ggcaaccgag gtgcggctgg catg
//
LOCUS       DAOTFI010000113        15037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4777_length_15037_cov_6.373381, whole genome shotgun sequence.
ACCESSION   DAOTFI010000113 DAOTFI010000000
VERSION     DAOTFI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 15037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15037
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_15037_cov_6.373381"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="P5558_12715"
     CDS             complement(<1..289)
                     /locus_tag="P5558_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY25220.1"
                     /translation="MPLDLDLVRARFPALRDGVAYFDNAGGSLVLASVAERVADYLLT
                     SSVQTGATYAASELAAARLLEARAKVARLINAARPEEIVLNATTTINLRFLA"
     gene            complement(317..988)
                     /locus_tag="P5558_12720"
     CDS             complement(317..988)
                     /locus_tag="P5558_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY25221.1"
                     /translation="MDIFETFFNLRVFQQALPLLLTGLWVTLLLGAASIVLGMAGGLL
                     LALARLYARSPLRQLAIVYIDVFRAIPLLVLLVIVYYALPFVGIRLSPFLSAVTALSL
                     VSSAYSAEIFRAGIEAVPQGQFEAARSLGLRPWPTMWKVILPQAVRIVIPPLTSNSIN
                     VLKDTALASVVAMPDLLKQATQAQALAANPTPLIAAAAIYLVILLPLVRLVALLERRF
                     ASQQR"
     gene            complement(1081..1878)
                     /locus_tag="P5558_12725"
     CDS             complement(1081..1878)
                     /locus_tag="P5558_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25222.1"
                     /translation="MLRRFSKPRFAALAMAAAMAFGTTPAVAQEAIVVSANIGNVPWE
                     FQDQSGAFVGFEIDLMVEVGERLGRPIETVNIPFQGLFAAVQSGRIDAATSSITITEE
                     RLKSVSFAQPYYDSDQSLTVRADSGIGGLADMSGKVVGVDTGSTGDMWATANTDAHGF
                     AEIRRYEGLNPAMLDLEAGRLDGYISDIPALLYYVRDKPQFEVVERIPTGERYSVMFA
                     KDAPLAAEVDEVITAMKEDGTLAAIHEKWFGKAPEAGTSTAEVLPMP"
     gene            2178..3191
                     /locus_tag="P5558_12730"
     CDS             2178..3191
                     /locus_tag="P5558_12730"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRY25223.1"
                     /translation="MENWANRKTLILSRSDMMGLVTPAEYVACVEQAYRMHGEGRFFM
                     EPKGHIVLDKYPGEWEAMPSYIEEPEAAACKWVSIRERNRERFNLPTVFSILIYTHPE
                     TGFPLAIVDGSYHTVMRTGAATAVSTKYLAREDARVLAIVGAGHMAEGTLETTATIRP
                     WTEVRVWSRSQKTLDGFLRNMQPKFPELTIRVSTNLEDVVPGADVVATITPARGPIVR
                     DEWIAPGTHIAAVGADKGGDQELESAILQRARIFVDDIRQCRTDGEINVPLSQGIIQE
                     SDIAGEIGKVVCGALPGRQSADQVTLFDSTGIALQDSATVPLEYERALAAGVGIEKKM
                     IST"
     gene            3380..4168
                     /locus_tag="P5558_12735"
     CDS             3380..4168
                     /locus_tag="P5558_12735"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HRY25224.1"
                     /translation="MMRLGAWASGLLAATVLTAGVPALAADFTIGLGGGIAPDYEGSD
                     EYEAVPNWLFSADDLYHPDTYFRVAGPTLRSNFVPDPHFRAGLNGLWVPERDDVENNQ
                     VDDLDSTDTALLLGPSIGWDFLAEPTRSLTTSIDLLYDVANSNGFLVTPKLSYFDALP
                     QSRFSYGAELSTTWASDDYMSEQFGINANNAAKSGLDQYSADESFKDVSVGVNGNYRF
                     TDKWSASLALQYKRMVGDAEDSPIVDDAGDANQFIAAATINFKF"
     gene            complement(4158..4655)
                     /locus_tag="P5558_12740"
     CDS             complement(4158..4655)
                     /locus_tag="P5558_12740"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY25225.1"
                     /translation="MTFTIRAASAADAGLLAPLVHALSLHERDPTEFLSEPGLARVLA
                     EGALEALVAEAGGAAIGYSLHHFGYESTYTAKGLYIVDLFVAEAHRRQGVGRALVGEV
                     ARLARARGGVFVWWTSKPANTEARAAYARLGAFTEPVLAHAVFDAAFDALADEAASRL
                     KQPRT"
     gene            4778..6100
                     /locus_tag="P5558_12745"
     CDS             4778..6100
                     /locus_tag="P5558_12745"
                     /inference="COORDINATES: protein motif:HMM:NF006543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HRY25226.1"
                     /translation="MAAFRYFSFFLAVVVLGTGAAGRAQEQDPAPAAEQPAEPGGEEP
                     VPAADYDAIQATIDRMRARLEGMGQAAAERDRALSFLEEQIDRAAGRIAGGDETQEAL
                     RQRTLTLNQELETLADEREQLTETVDQRAALIANLEQQVSRLTEMLAGERVLVGQLEG
                     DLDARDATLAEVTEQRETLEAELEATRAARQALDEELAALRQAQAEALAQRETKIAEL
                     EATVGQNETALGVADTRISALSRQLTELNRQLGAVESLLDSSENRIVEQQGTIASLGA
                     RLEQALLDRVEELSQYRSEFFGRLREVLGDRPEVRIVGDRFVFQSELLFASGSAWLGP
                     EGTRQLEALAETLREVAATIPPEIDWILRVDGHTDKVPVGANSAFRSNWELSTARAIS
                     VVEFLVRQGIPPERLAATGFGEFQPLDRRDDEIAYRRNRRIEFKLTEG"
     gene            6109..7044
                     /locus_tag="P5558_12750"
     CDS             6109..7044
                     /locus_tag="P5558_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRY25227.1"
                     /translation="MALHRIIIDCDPGQDDAVALLLALASPDEIEVLAVTAVAGNVPL
                     ALTEANARRIVELAGRPDIRVHKGCHRPLVKRLETAEYVHGETGLNGVELPPPSHPLA
                     PGHAVDAIIELVMAAEPKTVTLCPVGPLTNIAMALVKEPALAARLCQIVLMGGAIGLG
                     NVTPAAEFNIYVDPHAARVVFESGVPITMFGLDVTHQVLVTRPRLEAIRSLGTKVALA
                     AAGLIDFFNRFDSDRYEVDGAPLHDPCVIAHLIDPTLFKGKPCHVEIETEGRSMGRTN
                     VDWHPREQRPATATVMNAVDADRFFALLTERLGRL"
     gene            7061..7735
                     /locus_tag="P5558_12755"
     CDS             7061..7735
                     /locus_tag="P5558_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY25228.1"
                     /translation="MLHAFSVIALLALASDAGAQTPPSPTQVAAYDGLHLAAHQGDVT
                     TIRELAAAGADPDVRDAAGRTPAHVAAFASEDDALRALAAAGADLNALEGQAYDVVTI
                     AAVANDPELMSLAIELGNDPGLTTSPYDGTALIAAAHLGHQAVVRRLIEAGAPLDHVN
                     NLGWTALMEAVVLGDGGSDHQACVQALVDAGADPSIADRGGITPLEHARSRGYTEIVE
                     LIAGAG"
     gene            complement(7750..8757)
                     /locus_tag="P5558_12760"
     CDS             complement(7750..8757)
                     /locus_tag="P5558_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRY25229.1"
                     /translation="MPAEWAPHRRTWLAWPCRAALWGEHLEDAKHTYAAIAQAIAGFE
                     PVTVLARPDLTADASLHCGKGISVLPMQQDDSWVRDTGPAFLVSNDGRLGGVAWTFNG
                     WGETYPDHVEDARMARRMLEHVGAETFTTPLVLEGGGVHVDGEGTALLCERSVLDAGR
                     NPDRDRADVERELDGLLGIDKVIWLDGSLTDDETGGHIDNLACFSAPGKVLALDPATA
                     GEADRPGLERNLQTLRAATDAAGRSLEITLLPLPKPRKRADGRLLTLTYINFYVCNGA
                     VIVPVFEDYADETAKKLIGQAFPDRKLVPIDAAVLLQGGGGIHCVTQQQPLPPVVGDE
                     R"
     gene            8892..9359
                     /locus_tag="P5558_12765"
     CDS             8892..9359
                     /locus_tag="P5558_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY25230.1"
                     /translation="MSSRIEERLAELGLTLPVAAAPAANYVPWTRSGDTLYIAGQLPF
                     ENGKLEIKGAVGGAVSLEDGKRAARLCALNILAQAKAALDGDLDRVTRCLKLGAFVAA
                     AAGFGDHPQVVNGASDLMVELMGDAGRHARFAVGVSGLPLNAAVEIDAILEVR"
     gene            9370..10515
                     /locus_tag="P5558_12770"
     CDS             9370..10515
                     /locus_tag="P5558_12770"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY25231.1"
                     /translation="MATATHDASLVGPIGGIPAAAWDGLCPDGNPFLRHAFLAALEDS
                     GSAVAEAGWQPLHLTLGAPDSPTAAAPLYVKGHSYGEYVFDHGWADAYERAGGRYYPK
                     LLVAVPFTPVPGPRLLAPGDTLKLRLAAAIRGATDKLGLSSAHVNFAGEAEAALLEEE
                     GFLRRRGIQFHWHNRGYRDFQDFLDTLRSTKRKAIKKERAAVAASGVEIRTLTGDDLT
                     EADLAAFHPFYVATVDKRWGSAYLTADFFRRLARSFAERIVLVTVRQGGKLVGAALNL
                     RSGDTLYGRLWGCRREVEFLHFEACYYRAIDFAIQHGLARVEAGAQGMHKLQRGYEPV
                     FTHSAHYIRDPGLREPVRRFLRQESKAQAAELVELRGMLPFHREHEG"
     gene            complement(10512..11102)
                     /locus_tag="P5558_12775"
     CDS             complement(10512..11102)
                     /locus_tag="P5558_12775"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HRY25232.1"
                     /translation="MTGSFLRRCLALFAVLALAACGGTPPSRYYLMDAGPPAATAPAG
                     DTVVFVDRATVAAYADRSAMVVRRGTTEVAFAEQDAWAEPIAAQVTSVVTDALGDRFG
                     RANVVPTPVRLDREPDFRVVVEVLRFETGADGTALLDARWTLLEGPNDDFATAGRERL
                     TATPTDPASFDARAAALAATLVDLAGAIADAIEGRS"
     gene            complement(11099..12139)
                     /locus_tag="P5558_12780"
     CDS             complement(11099..12139)
                     /locus_tag="P5558_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRY25233.1"
                     /translation="MLRSWRRSCLVLVVLLACAPALPAGAQEALVEEIGRVLHPGEPA
                     PPLPEGTRFLVRFAEDVGGLEVGSMVTVRGLRIGTVREIAVVIDSEAATMDVPVVIDV
                     VPALIQVDGTRPTDPAAVEALAARLVAKGLRATLEKASPLGGTQRVALVFDSEAPPAV
                     LGSGGRYPEIPTAPTLGQKLETALGELVARVAALPLERIADEVLVTVTAVRDLATGPQ
                     LTHALAVVERAGNELAALATGPEVRGALASVGQSAQELQTLAAGLDARLTPAIDAILR
                     AAATIETAAGDGATTFAGLERSMGPRSALWGELLQTSRELAATTRSLRLLVEYLERHP
                     DALLRGRPETVP"
     gene            complement(12222..13697)
                     /locus_tag="P5558_12785"
     CDS             complement(12222..13697)
                     /locus_tag="P5558_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HRY25234.1"
                     /translation="MRCNRGVCRAIGLVLALVLGVVQAAVAEDAENELLEELQEPAFE
                     LPAVWQGDLDGIAERRLLRVLTTFSKTGYFLDGLTQRGATYELLQAFEKFLNEELKTK
                     KALPVRILMVPVDRDTLLPALAAGHGDIAAANLTITPERQATVDFADPIFASAREVVV
                     SGPAAGELSSLDDLAGTTVHLRASSSYWESVEKLNEDLAARGLEPIELVAVDGHLEDE
                     DLLEMVAAGILPWLVVDEHKAVLWVDIIPGLTLHDELALREDAAIAWALRRDTPKLKD
                     VVNRFTKTVEKGTATGNILFKRYYAENDWMRDPRATEDFERFQAAADLFRKYGDEYGF
                     DWLMLAAQGYQESRIDQSVRSPAGAIGVMQILPSTAADPNVGIPDITETESNIHAGAR
                     YLRFLSEQYLDDPALDDLNRTLFAFAGYNAGPGNLGKIRAEAESMGLDPNIWFGESEV
                     AAAKVIGRETTTYVANIAKYYYAYKLILVAEAAKAAVNPSG"
     gene            13864..>15037
                     /locus_tag="P5558_12790"
     CDS             13864..>15037
                     /locus_tag="P5558_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY25235.1"
                     /translation="MGAITIPEPDPGILGKRERILEGLRAILPGENLITDPRELVPYE
                     TDGFTAYARVPMAVVLPETTEEVSRILAFCSAEGVPVVPRGAGTSLAGGAIPREDCVV
                     LGVAKMSRVVAIDYANRTARVQSGITNIAISQAVAEEGFFYAPDPSSQLACTIAGNIA
                     MNSGGAHCLKYGVTTNNLLGVTLVLVDGTVVELGGEQLDAPGLDLLGLVCGSEGQLGV
                     VTEAVVRILPLAEGARPVLFGFPSSAAAGACVAEIIAHGIVPVAIEFMDKPAIAICEA
                     FAKAGYPLDVEAMLIVEVEGSDEEMDATLARIIEIARRHDVATIKESRSATETAQIWK
                     GRKSAFGATGRIADYICMDGTIPTGRLPEVLKRISEICTGYGLRVANVFHAGDGNLH"
BASE COUNT         2273 a         5189 c         5382 g         2193 t
ORIGIN      
        1 tcgcgaggaa gcgcaggttg atcgtggtcg tggcgttgag cacgatctcc tccgggcggg
       61 cggcgttgat cagccgggcg accttggcgc gcgcctcgag cagccgggcg gcggcgagct
      121 ccgacgcggc ataggtggcg ccggtctgga cgctgctggt gagcaggtag tcggcgaccc
      181 gctcggcgac gctcgcgagg acgagcgagc caccggcatt gtcgaaatag gcgacgccgt
      241 cacgcagggc cgggaaacgg gcgcggacga ggtcgagatc gagcggcatg gcggggtctt
      301 tcgggctgga ttggcttcag cgctgctggc tcgcgaagcg ccgctcgagc agtgccacca
      361 gccgcaccag cggcaggagg atgacgaggt agatggcggc cgccgcgatc aggggcgtcg
      421 ggttggcggc gagcgcctgg gcctgggtcg cctgcttcag gagatccggc atggcgacga
      481 ccgaggcgag cgccgtgtcc ttgagcacgt tgatcgagtt cgaggtcaag ggcgggatga
      541 cgatgcgcac cgcctgcggc aggatcacct tccacatggt gggccagggc cgaaggccga
      601 gcgagcgcgc cgcctcgaac tggccctgcg gcaccgcctc gatgccggcc cggaagatct
      661 cggccgaata ggcgctcgag acgagcgaga gggccgtcac cgccgagagg aagggcgaga
      721 gccggatgcc gacgaagggc agcgcgtaat agacgatgac cagcaggacc aggagcggga
      781 tggcgcgaaa gacgtcgata tagacgatcg cgagctggcg cagcggcgag cgcgcataga
      841 gccgggcgag ggcgaggagg agcccgcccg ccatgccgag cacgatgctg gcggcaccca
      901 ggagcagcgt cacccagagc ccggtgagca ggagcggcag cgcctgctgg aagacccgga
      961 ggttgaagaa cgtctcgaag atgtccaagg gctcaggtca ccggcggcaa gggcggggcg
     1021 cgggacgacg aggggagcgc gatcttccgg gccgcgctcc ccgatccgac ggtcgacgcc
     1081 ctacggcatc ggcagcacct cggccgtgct ggtgccggcc tccggggcct tgccgaacca
     1141 cttctcgtgg atggcggcga gcgtgccgtc ctccttcatc gccgtgatga cctcgtcgac
     1201 ctcggcggcg agcggggcgt ccttggcgaa catgaccgag tagcgctcgc cggtcgggat
     1261 ccgctcgacc acctcgaact gcggcttgtc gcggacatag tagagcagcg ccggaatgtc
     1321 ggagatgtag ccgtcgagcc ggcccgcctc gaggtcgagc atcgccgggt tgagcccttc
     1381 gtagcgccgg atctcggcga agccgtgggc atcggtgttg gcggtcgccc acatgtcgcc
     1441 ggtcgagccg gtgtcgacgc ccaccacctt gccggacatg tcggcgaggc cgccgatgcc
     1501 gctgtcggca cgcaccgtca gcgactggtc gctgtcgtag tagggctggg cgaaggagac
     1561 gctcttcagc cgctcctcgg tgatcgtgat ggacgaggtg gcggcgtcga tgcggcccga
     1621 ctggactgcg gcgaagaggc cctggaaggg gatgttgacg gtctcgatcg gccgcccgag
     1681 ccgctcgccg acctcgacca tgagatcgat ctcgaagccg acgaaggcgc cggactggtc
     1741 ctggaactcc cagggcacgt tgccgatatt ggcgctgacg acgatcgcct cctgggcgac
     1801 ggcgggcgtc gtgccgaagg ccatggccgc agccatggcc agggcggcga agcgaggctt
     1861 gctgaaacga cggagcatgt tggcttccag ttctggcaag gatgaacgac cgtcacgagg
     1921 ggccgtatcc gccacgtccg acatgctgcg agcgtccccg cccgcatgcc gccgactcgt
     1981 tcttctggcg ccacccccga tcccgtccga tgcaccggtt ctggaaccac ggggcctgcc
     2041 ggtcaaggcg acagccgagg acgtttccgt gccggcatcg ttcgggcctc ccggattgcc
     2101 gccggggaga acgggcttgc cgccgtggag cgttctgccg cacgctgggg cctggaccct
     2161 ggcacgggag cggaacgatg gagaactggg ccaaccgcaa gacgctgatc ctgagccgca
     2221 gcgacatgat ggggctggtc acgccggccg agtatgtcgc ctgcgtcgag caggcctacc
     2281 ggatgcacgg cgaggggcgc ttcttcatgg agcccaaggg gcacatcgtc ctcgacaagt
     2341 atccgggcga atgggaggcg atgccctcct atatcgagga gcccgaggcc gcggcctgca
     2401 agtgggtgtc gatccgcgag cgcaaccgcg agcgcttcaa cctgccgacg gtcttctcga
     2461 tcctgatcta cacccacccc gagaccggct tcccgctggc gatcgtcgac ggcagctacc
     2521 acacggtgat gcggaccggg gcggcgacgg cggtctcgac caagtacctg gcgcgcgagg
     2581 acgcccgggt gctggccatc gtcggcgccg ggcacatggc cgagggcacg ctcgagacga
     2641 cggccaccat ccggccctgg accgaggtcc gggtctggag ccgcagccag aagacgctcg
     2701 acggcttcct ccgcaacatg cagccgaagt tcccggagct cacgatcagg gtctcgacca
     2761 acctggagga cgtggtgccg ggcgccgacg tggtcgccac catcaccccg gcgcggggac
     2821 ccatcgtccg cgacgagtgg atcgccccgg gcacgcacat cgccgcggtc ggcgccgaca
     2881 agggcggcga ccaggagctg gagagcgcca tcctgcagcg ggcgcggatc ttcgtcgacg
     2941 acatccgcca gtgccggacc gacggcgaga tcaacgtgcc gctcagccag ggcatcatcc
     3001 aggagagcga catcgccggc gagatcggca aggtggtctg cggtgctttg ccggggcggc
     3061 agtcggcgga tcaggtgacc cttttcgatt cgaccggcat cgccctgcag gattcggcga
     3121 cggtgccgct cgagtacgag cgggcgctgg ccgcgggcgt cggcatcgag aagaagatga
     3181 tctcgacctg agcggccgtc gggccggctg tcacccattc gacacgccaa cgcaaaagcg
     3241 ccggggcagg ctttcgccat gctgtagctc cagccaagta gcgcgatagt atcggcgatc
     3301 ggttacgtgg ccgcatccgg cgggcagggt tccgtgcggc gtgttgcggc tgccggtcat
     3361 attcgaggag ttggtggtca tgatgagact tggtgcctgg gcgtccgggc tgctggcggc
     3421 gaccgtcctg acggcgggcg tgcccgccct ggcggcggac ttcacgatcg ggctgggtgg
     3481 cggcatagcg ccggactacg agggctccga cgagtacgag gccgtgccga actggctctt
     3541 ctcggccgac gatctctatc atccggacac ctacttccgg gtcgccggac caacgctgcg
     3601 ctcgaatttc gtgccggatc cgcattttcg cgccggcctc aacgggctct gggtgccgga
     3661 gcgcgacgat gtcgagaaca accaggtgga cgatctggac agcaccgaca cggcgctcct
     3721 gctcggcccg tcgatcggct gggatttcct cgccgagccg acccgcagcc tgacgacctc
     3781 catcgacctg ctctacgacg tggcgaacag caacggcttt ctggtcacgc cgaagctgtc
     3841 ctacttcgac gccttgccgc agagccggtt ctcctacggc gccgagctct ccaccacctg
     3901 ggcgagcgac gactacatgt cggagcagtt cggcatcaac gccaacaacg cggccaagag
     3961 cggcctcgac cagtacagcg ccgacgagag cttcaaggac gtgagcgtcg gcgtgaacgg
     4021 aaattaccgc ttcaccgaca aatggtcggc gtcgctggcg ctgcagtaca agcgcatggt
     4081 cggcgatgcc gaggacagcc cgatcgtcga cgatgccggc gacgccaacc agttcatcgc
     4141 agcggccacc atcaacttca agttctaggc tgcttcagcc gggacgcggc ctcgtcggcc
     4201 agcgcgtcga aggccgcgtc gaagacggcg tgggccagca ccggctcggt gaaggcgccg
     4261 agccgggcat aggcggcccg tgcttcggtg ttggcgggct tcgaggtcca ccagacgaag
     4321 acgccaccac gtgccctggc caggcgggcc acctcgccca ccagcgcccg gccgacgccc
     4381 tgccgccgat gcgcctcggc gacgaagaga tcgacgatgt agaggccctt ggccgtgtag
     4441 gtcgattcgt agccgaaatg gtggaggctg tagccgatgg cggcaccgcc ggcttcggcg
     4501 accagcgcct cgagcgcacc ttcggcgagc acgcgggcga ggcccggctc gctcaggaac
     4561 tcggtcgggt cgcgctcgtg caggctgagc gcatgcacca gcggtgccag aaggccggcg
     4621 tcggcggcgc tcgcggcgcg gatcgtgaag gtcatcgctc gggctccggt gcgacactcc
     4681 gggatggcgg cgggcttcta ccatgacgcc cctggaattg gccccctttc tctcgctcct
     4741 ggcgccgcgt aaggtcggcc gcaggaggtg gttgcgcgtg gcggctttca ggtatttttc
     4801 gttcttcctg gcggtggtcg tgctcgggac cggtgcggcg ggtcgtgcgc aggagcagga
     4861 tccggcgccg gcagcggagc agccggccga gccgggcggc gaggaaccag tgccggctgc
     4921 ggactacgac gccatccagg cgaccatcga ccggatgcgg gcccggctcg aaggcatggg
     4981 ccaggcggcg gccgagcgcg accgtgcgct ctccttcctc gaggagcaga tcgaccgggc
     5041 ggccgggcgc atcgccggcg gcgacgagac ccaggaggcg cttcgccagc gaacccttac
     5101 cctcaaccag gagctcgaga cgctcgccga cgagcgcgag cagctcaccg agacggtcga
     5161 ccagcgggcg gcgctcatcg ccaatctcga gcagcaggtg tcgaggctga ccgagatgct
     5221 ggccggcgaa cgggtgctgg tcggccagct cgaaggcgat ctcgacgccc gcgacgcgac
     5281 gctggccgag gtgacggagc agcgggaaac gctcgaggcc gagctcgagg cgacacgggc
     5341 ggcacggcag gcgctggacg aggagctggc ggcgctgcgg caggcgcagg ccgaggccct
     5401 ggcgcagcgc gagacgaaga tcgccgagct cgaggccacg gtcggccaga acgagaccgc
     5461 gctcggcgtg gcggacacgc ggatctcggc gctcagccgc cagctcaccg agctcaaccg
     5521 gcagctcggc gccgtcgaga gcctgctcga ctcgtccgag aaccgcatcg tcgagcagca
     5581 gggcacgatc gcctccctgg gtgcccggct cgagcaggcg ctgctcgatc gggtcgagga
     5641 gctgtcgcag taccgctcgg agttcttcgg ccggctgcgc gaggtgctgg gcgaccggcc
     5701 ggaggtgcgc atcgtcggcg accggttcgt cttccagtcc gagctgctgt tcgcctcggg
     5761 ctccgcctgg ctcggtcccg agggcacgcg gcagctcgag gccctggccg agaccctgcg
     5821 cgaggtggcg gccaccatcc cgcccgagat cgactggatc ctcagggttg acggccatac
     5881 cgacaaggtg ccggtcggcg ccaacagcgc cttcaggtcg aactgggagc tctcgacggc
     5941 gcgggcgatc tcggtggtcg agttcctggt gcgccagggc atcccgccgg agcgcctcgc
     6001 cgccacgggc ttcggcgagt tccagccgct cgaccggcgc gacgacgaga tcgcctatcg
     6061 ccgcaaccgg cggatcgagt tcaagctcac ggaaggatag gacggcgcat ggcgctccat
     6121 cgcatcatca tcgattgcga ccccgggcag gacgacgctg tcgccctgct gctggcgctg
     6181 gcctcgcccg acgagatcga ggtgctggcg gtgaccgccg tcgcgggcaa cgtgcccttg
     6241 gccctgaccg aggcgaacgc gcggcggatc gtggagctgg ccgggcggcc ggacatcagg
     6301 gtccacaagg gctgccaccg gccgctggtg aagcggctgg agacggcgga atacgtgcat
     6361 ggcgagaccg gcctcaacgg tgtcgagctg ccgccgccga gccatccgct ggcgccgggc
     6421 cacgcggtcg acgcgatcat cgagctggtc atggcggcgg agccgaagac cgtcaccctc
     6481 tgcccggtcg ggccactgac caacatcgcc atggcgctgg tcaaggagcc ggcgctcgcc
     6541 gcgcggctgt gccagatcgt cctgatgggc ggggccatcg gcctcggcaa cgtgacgccg
     6601 gccgccgagt tcaacatcta tgtcgacccg cacgcggcgc gcgtcgtctt cgagagcggc
     6661 gtgccgatca ccatgttcgg gctggatgtg acccatcagg tgctggtgac ccggccgcgg
     6721 ctcgaggcga tccggtcgct cggcaccaag gtggcgctgg cggcggccgg gctgatcgac
     6781 ttcttcaacc gcttcgacag cgaccgctac gaggtggacg gcgcccccct gcacgacccc
     6841 tgcgtgatcg cccacctgat cgacccgacc ctcttcaagg gcaagccctg ccacgtcgag
     6901 atcgagaccg aggggcgctc catgggccgc accaatgtcg actggcaccc gcgcgagcag
     6961 cgcccggcga cggccacggt gatgaacgcg gtggacgccg accggttctt cgcgctgctg
     7021 acggagcggc tcgggcggct ttgaccccct gaggataccg atgctgcacg ctttctccgt
     7081 catcgctctt cttgcgctcg cctcggatgc cggggcgcag accccacctt cgcctactca
     7141 ggtggccgcc tatgacgggc tgcatctggc ggcacaccag ggtgacgtga cgacgatccg
     7201 cgagctcgcg gcagcgggcg ccgatcccga cgtccgggac gccgccggcc gcacgccggc
     7261 ccatgtcgcc gccttcgcct ccgaggacga tgccctgcgc gccctcgcgg cggcgggtgc
     7321 cgacctgaac gcgctcgagg ggcaggccta cgacgtggtg accatcgccg ccgtggcgaa
     7381 cgaccccgag ctgatgtcgc tggcgatcga actcggcaac gatcccggcc tgaccaccag
     7441 cccctatgac ggcacggccc tgatcgccgc ggcccatctc ggccaccaag cggtggtgcg
     7501 gcggctgatc gaggcgggag cacccctcga tcacgtcaac aatctgggct ggaccgcgct
     7561 gatggaggcg gtcgtgctcg gcgatggcgg gtccgatcat caggcctgcg tccaggcgct
     7621 cgtcgacgcc ggcgccgacc cgtcgatcgc cgaccgcggc gggatcacgc cgctcgagca
     7681 cgcgcggtcg cggggctaca ccgaaatcgt cgagctgatt gcgggcgccg gctgagcggc
     7741 acgacaaccc tatcgctcgt cgccgaccac cggcggcaag ggctgctgct gggtgacgca
     7801 gtggatgccg ccgccgccct ggagcaggac ggccgcgtcg atgggcacca gcttgcggtc
     7861 ggggaaagcc tggccgatga gcttcttcgc ggtttcgtcg gcatagtcct cgaagaccgg
     7921 gacgatgacg gccccgttgc agacatagaa gttgatatag gtgagggtga gcaggcgccc
     7981 gtcggcccgc ttcctgggct tgggcagggg caggagcgtg atctcgaggc tgcggcccgc
     8041 cgcgtccgtc gccgcccgca gcgtctggag attgcgttcg aggccgggcc ggtcggcctc
     8101 gccggcggtc gccgggtcga gggccaggac cttgcccggg gcggagaagc aggcgaggtt
     8161 gtcgatatgg cccccggtct cgtcgtcggt caggctgccg tcgagccaga tcaccttgtc
     8221 gatgccgagc aggccgtcga gctcgcgctc gacgtcggcg cggtcgcggt cggggttgcg
     8281 gccggcgtcg aggaccgagc gctcgcagag cagcgccgtg ccctcgccgt cgacatgcac
     8341 gccgccgccc tcgaggacga gcggcgtggt gaaggtctcg gcgccgacat gctcgagcat
     8401 gcgccgggcc atgcgcgcat cctcgacgtg gtcgggatag gtctcgcccc agccgttgaa
     8461 ggtccaggcg acgccgccga gccggccgtc attgctgacg aggaaggcgg ggccggtgtc
     8521 cctgacccag ctgtcgtcct gctgcatggg cagcaccgag atgcccttgc cgcagtgcag
     8581 ggaggcatcg gcggtgaggt cgggccgggc gaggacggtc accggctcga acccggcgat
     8641 cgcctgggcg atggcggcgt aggtgtgctt cgcatcctcg agatgctcgc cccagagggc
     8701 ggcgcggcag ggccaggcga gccaggtgcg ccggtgcggc gcccattcgg ccggcatgcg
     8761 aaatccgttc tggcgtggcg tgcccaaatc gtccctccgg cctttttgcg accctgtcac
     8821 tttgcccacc gcaaggttgc ggtatatggc ttgccgacgc aatcggcgat gatggggaag
     8881 gacgcatatc gatgtcgagc cgaattgagg agcgcctggc cgagctcggc ctgaccctgc
     8941 cggtggcggc agcgccggcg gcgaactacg tgccctggac ccgcagcggc gacacgctct
     9001 acatcgccgg ccagctgccc ttcgagaacg gcaagctgga gatcaagggt gcggtcggcg
     9061 gggccgtgag cctcgaggac ggcaagcgtg cggcccggct ctgtgctttg aacatcctgg
     9121 cccaggccaa ggcggcgctc gacggcgacc tcgaccgggt gacccgctgc ctcaagctcg
     9181 gcgccttcgt cgccgcggcg gcgggcttcg gcgatcatcc gcaggtggtc aacggcgctt
     9241 cggacctcat ggtcgagctg atgggcgacg ccggccggca tgcccgcttc gcggtcggtg
     9301 tcagcggcct gccactcaac gccgcggtcg agatcgacgc catcctcgaa gtgcgctgac
     9361 ggcgcagtct tggcgacggc gacccacgac gcgagcctgg tcggcccgat cggcggcatt
     9421 ccggcggccg cctgggacgg cctctgcccg gacggcaacc cgtttttgcg ccatgccttc
     9481 ctcgccgccc tcgaggactc gggctcggcg gtggccgagg ccggctggca gccgctgcac
     9541 ctgacgctcg gcgcgccgga ctccccgacg gccgccgccc cgctctacgt caagggccat
     9601 tcctacggcg agtacgtctt cgatcacggc tgggccgacg cctatgagcg ggccggcggg
     9661 cggtattatc ccaagctgct ggtggcggtg ccgttcacgc cggtgccggg cccgcggctc
     9721 ctggccccgg gcgacacgct gaagctgcgg ctcgccgccg ccattcgcgg ggcgaccgac
     9781 aagctcgggc tgtcgtcggc gcatgtcaat ttcgccggcg aggccgaggc ggcgctgctc
     9841 gaggaggagg gcttcctgcg ccgccgcggc atccagttcc actggcacaa ccggggctat
     9901 agggacttcc aggatttcct cgacaccctg cgctcgacca agcggaaggc gatcaaaaag
     9961 gagcgggccg ccgtggcggc gtccggggtg gagatccgca cgctcaccgg cgacgacctc
    10021 accgaggccg atctcgcggc cttccatccc ttctacgtcg cgaccgtcga caagcgctgg
    10081 ggcagcgcct acctcaccgc cgatttcttc cgccggctgg cccggagctt cgccgagcgg
    10141 atcgtgctgg tgaccgtccg ccagggcggc aagctggtcg gtgccgccct caacctgcgc
    10201 agcggcgaca cgctctacgg ccgcctctgg ggctgccgcc gcgaggtcga gttcctgcat
    10261 ttcgaggcct gctactaccg cgccatcgac ttcgcgattc agcacggcct cgcccgggtc
    10321 gaggccggtg cgcagggcat gcacaagctg cagcgcggct acgagccggt cttcacccac
    10381 agcgcccact acatccgcga cccgggcctc agggagccgg tgcgccgctt tctccggcag
    10441 gagtcaaagg cccaggcggc cgagctggtc gagctgcgcg ggatgctgcc gttccatcgc
    10501 gagcacgagg gctaactcct gccctcgatc gcgtccgcaa tcgccccggc caggtcgacc
    10561 agcgtcgccg cgagggccgc agcgcgggcg tcgaaggagg cggggtcggt gggtgtggcg
    10621 gtcaggcgct cgcggccggc ggtggcgaaa tcgtcgttcg gcccctcgag cagggtccag
    10681 cgggcgtcga gcagcgctgt gccgtcggcg ccggtctcga agcgcagcac ctcgacgacg
    10741 acgcggaaat ccggctcgcg gtccagccgc accggggtgg gcacgacatt ggcgcggccg
    10801 aagcgatcgc cgagcgcatc ggtgacgacg ctggtgacct gggcggcgat gggctcggcc
    10861 caggcgtcct gctcggcgaa ggcgacctcg gtcgtgcccc ggcggacgac catggccgag
    10921 cggtcggcat aggccgccac cgtcgcccgg tcgacgaaga cgaccgtgtc gccggccggt
    10981 gccgtcgccg ccggcgggcc ggcatccatc aggtagtagc gggacggcgg cgtcccgccg
    11041 caggcggcga gcgccagcac cgcgaacaag gcaaggcagc gccggaggaa gcttcccgtc
    11101 atggcacggt ctccgggcgg ccgcgcagga gcgcgtcggg gtggcgctcg agatactcga
    11161 ccaggagcct gagcgagcgg gtcgtggcgg cgagctcgcg gctggtctgc agcagctcgc
    11221 cccacaaggc tgacctcggc cccatcgagc gctcgaggcc ggcgaaggtc gttgccccgt
    11281 cgccggctgc cgtctcgatg gtggcggcgg cgcgcaggat ggcgtcgatc gccggcgtca
    11341 gccgggcgtc gagaccggcg gcgagggtct ggagctcctg cgccgactgg cccaccgagg
    11401 cgagcgcccc tctgacctcc gggccggtcg cgagggcggc gagctcgttg ccggcgcgct
    11461 cgacgacggc cagcgcatgg gtcagctggg gcccggtggc gaggtcgcga acggcggtga
    11521 cggtcaccag gacctcgtcg gcgatccgct cgagcggcaa ggctgcgacc cgggccacga
    11581 gctcgcccag agccgtttcc agcttctgcc ccagggtcgg cgccgtcggg atctcggggt
    11641 agcggccacc gctgccgagc acggcgggcg gcgcttccga atcgaagacc agggcgacgc
    11701 gctgcgtgcc gccgagcggc gacgccttct ccagcgtggc acgcaggccc ttggccacga
    11761 ggcgcgcggc cagcgcctcg accgccgccg ggtcggtcgg gcgggtacca tccacctgga
    11821 tcagcgccgg caccacgtcg atcaccaccg gcacgtccat ggtcgccgcc tcgctgtcga
    11881 tgaccacggc gatctcgcgc acggtgccga tccgcagccc ccggaccgtg accatcgagc
    11941 ccacctcgag cccgcccaca tcctcggcaa agcggacgag gaagcgggta ccctcgggca
    12001 agggtggcgc cggctcgccc ggatgcagca cccggccgat ttcctcgacc agggcttcct
    12061 gcgccccggc cggaagtgcg ggtgcgcagg ccagcaggac gacgaggaca aggcaggagc
    12121 ggcgccacga tcgaagcatg gcgcgatgtt gccggtatcg gccggcatcg gcaagcggcc
    12181 atcgggccga gccgcggcca agccccgttc gggacgggcg ctcagcccga cgggttcacc
    12241 gccgccttcg cggcctcggc cacgaggatc agcttgtagg catagtagta cttggcgatg
    12301 ttcgcgacat aggtggtcgt ctcgcggccg atcaccttcg ccgccgccac ctccgactcg
    12361 ccgaaccaga tgttcggatc gagccccatg ctctcggcct cggcccggat cttgccgaga
    12421 ttgcccggcc cggcattgta gccggcgaag gcgaagagcg tgcggttgag gtcgtcgagc
    12481 gccgggtcgt ccagatactg ctcgctcagg aagcgcagat agcgggcacc cgcgtggatg
    12541 ttgctctcgg tctcggtgat gtccggaatg ccgacattcg ggtccgccgc cgtgctcggc
    12601 aggatctgca tcaccccgat ggcccccgcc gggctgcgca cgctctggtc gatccgcgat
    12661 tcctggtagc cctgggccgc cagcatcagc cagtcgaagc cgtattcgtc gccgtatttc
    12721 cggaagaggt cggcggctgc ctggaagcgc tcgaaatcct cggtggcgcg cggatcgcgc
    12781 atccagtcgt tctcggcata gtagcgcttg aagaggatgt tgccggtggc ggtccccttc
    12841 tccacggttt tggtgaagcg gttgaccaca tcctttagct ttggcgtgtc ccggcgcagc
    12901 gcccaggcga tcgccgcgtc ctcgcgcagc gccagctcgt cgtgcagcgt caggcccggg
    12961 atgatgtcga cccagagcac ggccttgtgc tcgtcgacga ccagccaggg caggatgccg
    13021 gccgccacca tctcgagcag gtcctcgtcc tcgagatgcc cgtcgacggc gacgagctcg
    13081 atcggctcga ggccgcgggc ggcgaggtcc tcgttcagct tctccacgct ctcccagtag
    13141 ctgctcgagg cgcgcagatg gacggtggtg ccggcgagat cgtcgaggct cgaaagctct
    13201 cctgccgccg gtccgctgac caccacctcg cgcgcgctgg cgaagatggg gtcggcgaag
    13261 tcgaccgtgg cctgccgctc cggcgtgatg gtcaggttgg cggcggcgat gtcgccatgg
    13321 ccggccgcca gcgccggcaa gagcgtgtcg cggtcgacag gcaccatgag gatgcggacc
    13381 ggcagcgcct tcttcgtctt cagctcctcg ttgaggaact tctcgaaggc ctgcaacagc
    13441 tcgtaggtag cgccgcgctg ggtcagcccg tcgaggaaat agcctgtctt gctgaaggtc
    13501 gtgagcaccc gcaggagccg ccgctcggcg atgccgtcga gatcgccctg ccagaccgcc
    13561 ggcagctcga aagccggctc ctgcagctcc tcgaggagct cgttctccgc atcctccgcc
    13621 acggccgcct gcacgacgcc gagaaccagc gccaggacaa gcccgatcgc gcgacaaacg
    13681 ccccggttgc accgcatgtt tcgttccgct gcaacattcc gcccggatcc aattatacat
    13741 tttccgtccg ccattccgca acgggctgcc tcggccggtc tgctcccgga aagccgcagc
    13801 acgatgcttg tgaccgggcg gcccgcatgc cacttgaaag ggagcaatgt ggaggcagca
    13861 ccaatgggcg ccatcaccat ccctgagccc gatccgggga tactcggcaa gcgcgagcgc
    13921 atcctcgaag ggctgcgcgc catcctgccg ggcgagaacc tgatcaccga cccgcgcgag
    13981 ctcgtcccct acgagaccga cgggttcacg gcctatgcga gggtgcccat ggccgtcgtc
    14041 ctgcccgaga cgacggagga ggtgagccgc atcctcgcct tctgctcggc cgagggggtg
    14101 cccgtcgtgc cgcgcggcgc cggcacgtcg ctcgccggcg gggccatccc gcgcgaggac
    14161 tgcgtcgtcc tcggcgttgc caagatgagc cgggtggtcg ccatcgacta cgccaaccgc
    14221 accgcccggg tgcagagcgg gatcaccaac atcgccatca gccaggcggt cgccgaggag
    14281 ggcttcttct acgcccccga cccctcctcg cagctcgcct gcaccatcgc cggcaatatc
    14341 gccatgaact cgggcggtgc ccactgcctc aaatacggcg tcaccaccaa caacctgctg
    14401 ggcgtcacgc tggtcctggt cgacggcacg gtggtggagc tcggcggcga gcagctcgac
    14461 gccccgggcc tcgatctcct cggcctcgtc tgcggctcgg agggccagct cggcgtcgtc
    14521 accgaggccg tcgtccgcat cctgccgctg gccgagggtg cccggcccgt gctcttcggc
    14581 ttcccttcct ccgcggcggc aggcgcctgc gtcgccgaga tcatcgcgca cggcatcgtg
    14641 ccggtcgcca tcgagttcat ggacaagccg gcgatcgcca tctgcgaggc cttcgccaag
    14701 gcgggctacc cgctcgatgt cgaggccatg ctgatcgtcg aggtggaggg ctccgacgag
    14761 gagatggacg ccacgctggc gcggatcatc gagatcgccc gccgccacga cgtggcgacc
    14821 atcaaggaga gcaggtccgc caccgagacc gcgcagatct ggaagggccg caagtccgcc
    14881 ttcggcgcca ccgggcggat cgccgactat atctgcatgg acggcaccat cccgaccggc
    14941 cggctgcccg aggtgctgaa gcgcatcagc gagatctgca ccggctacgg gctgcgcgtc
    15001 gccaatgtct tccacgccgg cgacggcaat ctccacc
//
LOCUS       DAOTFI010000114        15009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4790_length_15009_cov_6.128527, whole genome shotgun sequence.
ACCESSION   DAOTFI010000114 DAOTFI010000000
VERSION     DAOTFI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4790_length_15009_cov_6.128527"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="P5558_12795"
     CDS             complement(<1..819)
                     /locus_tag="P5558_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY25236.1"
                     /translation="MRYATTRRPVLTLTTGPVDAYPEVLQALATTVLYDFDPAFLAHY
                     EKVARKAQRAMGTGGVPTILHGEPVLALEAAAASLIAAGDTVLNLVSGVYGKGFGHWA
                     KRWCRELVEITVPYDDVIDPEAVAAMFRERPDIAVVSVVHHETPSGTINPVDEIGAIC
                     ARHGAHLIVDAVSSWGGMPASPDACQAAIYVTGPSKCLGCPPGLSLVAVSERGWAKMA
                     ANPKAPRASMLSILDWQEAWRSDRPFPFTPSVAEINGLDRALDLYLEEGPEAVWG"
     gene            complement(839..1087)
                     /locus_tag="P5558_12800"
     CDS             complement(839..1087)
                     /locus_tag="P5558_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25237.1"
                     /translation="MTEAKACLLDGYHVFWNEGPGGDASYTRKRLELLCQASFPIDPA
                     AGAGSLWKLKKIAEETGAELRFGHDMEQFETYKKAPRF"
     gene            complement(1104..2039)
                     /locus_tag="P5558_12805"
     CDS             complement(1104..2039)
                     /locus_tag="P5558_12805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25238.1"
                     /translation="MEALLTAAFLTALLAGAVAAGVPLLLAGLGEQMSEKAGVLNIGI
                     EGMMLMGAYLGFLVAFESGNVALGFVAGMAGGMAVALVMAILCVGLGLNQIVIGIGLT
                     LGVEGLTALLHHFQFSTTYPRLPAAWRVPVPGLVELPVVGPALFDRHPLVYLAFLLVP
                     LLAWLYRRTALGLTLRAAGEKPAALDVAGIDVATVRFLAVLFTGAMAGLGGAFMANVA
                     AGIFIPFMTGGAGFIGIVLAMLARGRPAWVLIGAVVFGGALSLTTALQVAGLNVPTDL
                     IQMLPFAAVMAVLVLYGRKARLPAALGLPYVRGAR"
     gene            complement(2039..3121)
                     /locus_tag="P5558_12810"
     CDS             complement(2039..3121)
                     /locus_tag="P5558_12810"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25239.1"
                     /translation="MSASLETTLDEGGADTSGPRRALAHLALVTLGPILAALVLSGLV
                     LLALGVDPFAYYGYVAQRGLFSWLGLQQTLTRMAPLLCLAAALIVAFRAGIWNLGIDG
                     QFLLGAVTSAALAPFLITVLPAGAVLLVAAAGAALVGALWSLLPALLRAYQQVNEIIT
                     TLMMTFLGVSFANLLVKLVFLDPGTTVPQTRTLPVADRLPTLFGTTISGGLVLGLVAV
                     VVVHLVMTRTAFGLKLRTVGENPLAARHAGLDVPLLTVAVFAISAGFAGLAGGIEILG
                     VHGNLQAGWNPAYGLAVIPLVFLARFNGFAAIVFVFFYSALMIGGESAARRIGVPHYF
                     TLVLVANLLILLALAEYLDHRRRRAG"
     gene            complement(3118..4734)
                     /locus_tag="P5558_12815"
     CDS             complement(3118..4734)
                     /locus_tag="P5558_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25240.1"
                     /translation="MAAPSVPLGTMQAAATPARPVPATAPMVELLGVTKRFPGVLAVD
                     RVDLALLPGEVHALLGENGAGKSTLVAMLSGLVEPDAGRIRVAGEERRIASPRAALQQ
                     GISTVFQHVMLVPTLTVLENLVLGMSWWRRPDRAELEQRLAGIRSAFGIALDLDQTTG
                     ELSLGEQQQIEIVRALLRRSRVLILDEATSMLTPQGADELGALMRRLVKSGLAVVFIT
                     HKLDEACRFGDRITVLRLGRKVGELAPEGLAAMDQAAAVEEVVGLMFGDRSIAEARPS
                     RLRKAEGPPVLSVRGLTVTGATRLARADDVSFELRPGEILGIAGIDGNGQKQLAEALA
                     GQRTAAAGSIVLDGRDITRAGVAERRRLGLRYLTDDRIGEGAVGGFPVATNAVLKEIG
                     APPFWSGGPFLLGGIERPGRIAAHARELIDRYDVRTPDEATPIGRLSGGNIQKVLLAR
                     ELSGDARVVVFNKPTHGLDVQNIMASRNRIAAIADAGLAVLLISTDLEELLDVADRIA
                     VMAAGRLTGIVANDEGARARIGALMIGRAA"
     gene            complement(4755..5786)
                     /locus_tag="P5558_12820"
     CDS             complement(4755..5786)
                     /locus_tag="P5558_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRY25241.1"
                     /translation="MIKRLMQGAALGLALATFGAVVANAVQVNAIAILVPEEGTDYGW
                     NQQGVDAAVAAANATGVEVITAEGLGYGDIRPTLRELAEEGASLMIAHASGYNTAAPE
                     IAQETGVPVAIVDSPDALVPGLVADYTLSGHEGAYLAGVMAATMTRTGTVGIVVSGEP
                     PSWNAQSAGFAQGVQATNPDVKIVYAVIGPAAYADAAGGNRVTAAVIAAGADIVFGQG
                     NGSSFGMLQAIETTPATDGGKVLFIDVIGDKSSIDKGYLLTSVLWDMTPVYTAMIEDL
                     ENDTFGTKSYAIGLADDSVRLLQTDNIPADVWEQVMAVRQELVDGTLVLEPVFDAEGV
                     RALVTSVAE"
     gene            5839..6234
                     /locus_tag="P5558_12825"
     CDS             5839..6234
                     /locus_tag="P5558_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25242.1"
                     /translation="MLCCLLALLPAMPAAGSELVPLAFSVRNLATEAITCAATLAHWF
                     SIELGTAEAGERVVAELWHDPDSGAVLILNEQGEAMPIEALWCGLAGRSWQTRSPIPL
                     TRNPGAAPVGIELDCRMAGGRLACNDVRR"
     gene            complement(6280..7230)
                     /locus_tag="P5558_12830"
     CDS             complement(6280..7230)
                     /locus_tag="P5558_12830"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRY25243.1"
                     /translation="MASPAPAIRKVAVIGLGSMGPGMMARLARGGLEVSGFDIAEAAR
                     ERATGLLSRAAAVLDALAIAPPTAGEGAIRIEGSLEETVAGADLVIENVPEKIETKAA
                     LYRQVEPLIDQAAILATDTSGIPITSLQAHVAHPRRFVGMHWSNPPHIIPMIEVIGGA
                     ATAPGTVAAVRGLIHGLGLLPVVIKKDVPGFVENRVLYALLRECVDLVEGGVIDAEGL
                     DTVVRWGIGMKLAVVGPMRLLDMAGLDIYEAVSSFLNKELADRADVSPLVTAATARGR
                     LGMKTGGGLFDYTREELERLPAERARALVAVRRVLESGGG"
     gene            complement(7234..7836)
                     /locus_tag="P5558_12835"
     CDS             complement(7234..7836)
                     /locus_tag="P5558_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY25244.1"
                     /translation="MIRLLGRATSGNVQKILFMLEELGIPYEREDFGRQFGNTATAEY
                     LAMNPAGKVPTLVDGEVVVWESNTILRYLAATQGKDLAGGTPAEASVVERWMDFLLAA
                     INPGYMAAFKGSKLPPAERAAGYDEQVKDLVANLAVLDRHVSGKKFFALGKLTVADIA
                     LAPIVKRCLDFPLDRPSFVGLEAWMAGIAERPAFKTATGG"
     gene            complement(7902..8303)
                     /locus_tag="P5558_12840"
     CDS             complement(7902..8303)
                     /locus_tag="P5558_12840"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY25245.1"
                     /translation="MTDKVGRSDRHYAIGKKNPNLPFHPAVRAGDFIFVSGQVSKDEN
                     GTMLTGDIETETAGTIRAIERILAEEGATLKDVVRVCTYLEDTRDFGRYNKVFGQMFE
                     GAVLARTTVEARAVIATRIEMDAVAYKPLGD"
     gene            complement(8320..9447)
                     /locus_tag="P5558_12845"
     CDS             complement(8320..9447)
                     /locus_tag="P5558_12845"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25246.1"
                     /translation="MARSRNTELLSHIDCPGGGQVWVDGTTLYVGHMRAPTGTSVYDV
                     SDPKSPRQIARLDLPPGWHSHKVRVANGLMLVNHERQGQAGDPAFGGGLAVYDVSRPA
                     EPRLLSKWSGGGRGVHRFDFDGRHAYISPTVEGYIGNIVVILDLADPANPKEVGRWWV
                     EGQWQAGGEEYPWGEWPQPRCHHPLRQGDRLYVSYWHHGLFILDISELSSPKLVSAVN
                     TSPVFPHPTHTCLPIPETLKGRRVMVVADEDVAKLWPAAPAFTWIYDITEERLPTPIA
                     TFQVEGPEFDGSPQEPMTGCHQPSERFAGTVIPFAWFAQGLRLVDIRDPFRPVEVGHF
                     LPDVPPGADRPSSNDVTIDERGLVYLVDRIHGVDIIETTAF"
     gene            complement(9461..10606)
                     /locus_tag="P5558_12850"
     CDS             complement(9461..10606)
                     /locus_tag="P5558_12850"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY25247.1"
                     /translation="MVDAAAAVHNRANALEDLYDEIHERSMFPFWATSEGVEHDEIKQ
                     LMATQKAIPHLWRWREDILPLLERAAELITMNDSERRSLILINPGLAPRRATVSTMYT
                     AYRLNDADEIMPPHKHSPNAIRLGLTGTGNFTGVEGENVVFGPGDMVLTPNDAWHNHG
                     TVGHQQATNLSVLDLPLVETLNAIHFDHDYMEEEGGKLVKKKEQSARYPADYSQAVYG
                     KGGMVPRFVDQHRGGGLSSPMYVYRWAAMEEILQKHKDWDGDPHEALMVEYTDPFRGG
                     PVFKTMTFFMQMLRPGEKTLALKQTASLLVSPFRGEGASIVDGQRVDWDQFDTLAVPG
                     GSWCSHENTSATEPLYLFVASDEPVLKALSLYKKWGRTPDGDVARLA"
     gene            complement(10717..12339)
                     /locus_tag="P5558_12855"
     CDS             complement(10717..12339)
                     /locus_tag="P5558_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY25248.1"
                     /translation="MATSEFDYVIVGAGSAGCVLANRLTACGRHRVLLLEAGGWDRHP
                     WIHIPLGWGKILQKRLFDWGYFCESEASVDGRAVECARGRVVGGCSSTNAMAHVRGNR
                     ADYDRWAATGLPDWSYDAVLPYFKKQESWAGGADAWRGGDGPIGVRPCRYQDELVKAF
                     AAAGREAGHGWTDDYNGADQLGFSRLQMTIKGGLRCSAATGYLRPVLQRPNLTVVTGA
                     HVQKLELSGKRATGVGYRVGEIGHTARAAREVLLAAGAINSPQLLQLSGIGDPAALSA
                     LGIPVHTALNGVGRNLQDHVSAVLMYRRKSPGPFQHMMRADRIVPDLIRTRLGGDGFS
                     GDVPGGTVAFLRSSLAESPAPDLQLLFTAAPLAAWPWLEPFKRPFPDGFVMRIVLVRP
                     SSRGAVSLRSADPGAAPVIRQNFLSEAREWRILREGIGMARDLAGRAALRPFVEAEIA
                     PGAGKTSEAALDAHIRATSITVHHPGGTCRMGPSPEAGAVVDGELRLHGVEGLRVVDA
                     SVMPDLPSGNINAAVMMIAEAAAERILASAAA"
     gene            12483..13226
                     /locus_tag="P5558_12860"
     CDS             12483..13226
                     /locus_tag="P5558_12860"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY25249.1"
                     /translation="MTTETIMAPGVPASDVDVASMDLPGTLPGEPAGLERMMAAILER
                     YREAGELPPERQLRDELAVTRHRLRTALARLRAEGRLPPARTGRRPVGATQVEGSEFL
                     ARLANPLELMELRLIIEPTLARLTALRASSLEIARVVKLATTPPGSPAAATDIAFHRA
                     VAQASRNTLAGEIYALLRRIGTDQRIRVAGSDTSCPRRIRERDGEHQRVAAAIAARDG
                     AAAEQAMREHLLAVQRRILERTAAGGAVA"
     gene            complement(13247..13579)
                     /locus_tag="P5558_12865"
     CDS             complement(13247..13579)
                     /locus_tag="P5558_12865"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRY25250.1"
                     /translation="MPSQNARVLRHIVLFAFKPEATPPEIASVEAAFAALPGQIPEII
                     DFEWGTDVSIEGRAQGFTHCFLVTFADEAGREAYLPHPAHQAFVALVGQHREKVLVID
                     YWARSTAG"
     gene            13752..14015
                     /locus_tag="P5558_12870"
     CDS             13752..14015
                     /locus_tag="P5558_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY25251.1"
                     /translation="MMTAGQLRAARALLGIDQRTLAELAGLSVPTIQRMEKSDGVVRG
                     NVDSLMKLIEALEVAGVALIADGPDGGRGVRLKAAPPADDQAP"
     gene            14012..>15009
                     /locus_tag="P5558_12875"
     CDS             14012..>15009
                     /locus_tag="P5558_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY25252.1"
                     /translation="MTTSLVLIATALLLATAALAVARAGHKRIHDIVYGACLALMTAI
                     LVIALAALLTGSGSALVLPLGLPWIGARFRIDALAAFFLALLGAGGAFASLYGQGYGR
                     HEAAPERVLPFYPLFLAGMTLVLLADDAFTFLLAWEVMSLASWALVLTQHRVADNRRA
                     GYVYLAMASAGTVALLLAFGLLAGATGAYGFAEIRDATPAPLTATLVLLLALFGAGSK
                     AGLVPLHVWLPLAHPAAPSHVSALMSGVMTKVAIYGFLRLVLDLLGEPPWWGALVVLF
                     MGGATAVIGILMALMQRDLKRLLAYSTIENVGVVFTGIGLALAFEASGMAAAAALAL"
BASE COUNT         2256 a         5341 c         5209 g         2203 t
ORIGIN      
        1 cccccagaca gcctccggac cctcctcgag atagaggtcg agagcccggt ccaggccgtt
       61 gatctcggcg accgagggcg tgaacgggaa gggccggtcg ctgcgccagg cctcctgcca
      121 gtcgaggatg ctgagcatcg aggcccgtgg cgccttcggg ttcgccgcca tcttggccca
      181 gccccgctcg ctcacggcca ccaggctgag gccgggcggg cagcccaggc acttgctcgg
      241 gccggtgacg tagatggcgg cctggcaggc atccggcgag gccggcatcc ctccccagga
      301 ggagaccgcg tcgacgatga gatgcgcacc atgccgcgcg cagatcgccc cgatctcgtc
      361 gaccggattg atcgtgccgg aaggcgtctc gtggtggacg acagaaacca cggcgatgtc
      421 cggccgctcc cggaacatgg ccgccaccgc ttcggggtcg atgacgtcat cgtacgggac
      481 cgtgatctcg acgagctccc tgcaccagcg cttcgcccag tggccgaagc ccttgccgta
      541 gacgcccgac acgaggttga ggacggtatc cccggcggcg atcaaggacg cagccgcggc
      601 ttccagcgcc agcaccggct cgccgtgcag gatggtcggc acgccgcctg tgcccatcgc
      661 ccgctgcgcc ttcctcgcca ccttctcgta gtgggcgagg aaggccgggt cgaagtcgta
      721 gagcacggtg gtggcgagcg cctgcagcac ctcgggatag gcatcgaccg ggcccgtggt
      781 cagggtcagg accggccgtc tcgtcgtggc gtagcgcatg accgcctccc cttcagccct
      841 agaaccgggg cgccttcttg taggtctcga actgctccat gtcgtggccg aacctgagct
      901 ccgcacccgt ctcctcggcg atcttcttca gcttccacaa cgagccagcg ccggccgcgg
      961 gatcgatggg gaagctcgcc tggcagagca gctcgagcct cttcctcgtg tagctggcgt
     1021 cgccgccggg gccctcgttc cagaagacat ggtagccgtc gagcaggcag gccttggctt
     1081 cggtcatcgg gtgagctcct ccgctatctg gcaccgcgaa catagggcag gcccagggcg
     1141 gccggcaggc gggccttgcg gccgtagagg acgagcaccg ccatcacggc ggcgaagggc
     1201 agcatctgga tgaggtcggt cggcacgttg agcccggcga cctggagcgc cgtggtcagc
     1261 gacagggcac cgccgaagac gaccgccccg atcagcaccc aggccgggcg gccgcgcgcc
     1321 agcatggcga gcacgatgcc gatgaacccg gccccgccgg tcatgaaggg gatgaagatg
     1381 ccggccgcga cattggccat gaaggcgccg ccgagcccgg ccatggcccc ggtgaagagc
     1441 acggcgagaa agcgcacggt agcgacgtcg atgccggcga catcgagagc ggccggcttc
     1501 tcgccggcgg cccgcaaggt caggccgagc gccgtccgcc ggtagagcca ggccaggagc
     1561 ggcacgagca ggaaggcgag gtagacgagc gggtgccggt cgaagagggc ggggccgacg
     1621 accggcagct ccacgaggcc cggcacgggc acccgccagg cggccggcag gcgcggatag
     1681 gtcgtgctga actggaagtg gtggaggagc gcggtgagcc cctcgacgcc gagcgtcagg
     1741 ccgatgccga tgacgatctg gttgagcccg agcccgacac agaggatcgc catgacgagc
     1801 gccaccgcca tcccgccggc catcccggcg acgaagccga gcgccacgtt gccgctctcg
     1861 aaggcgacga gaaagcccag ataggcgccc atcagcatca tgccctcgat gccgatattg
     1921 agcacccccg ccttctccga catctgctcg ccgagtccgg caagcagcag cggcacgccg
     1981 gcggcgacgg ccccggcgag gagggccgtg aggaaggccg cggtcaggag tgcttccatc
     2041 agcccgcccg ccgccggcgg tggtcgagat actctgccag cgccaggagg atgagcaggt
     2101 tggcgacgag gacgagggtg aagtaatgcg gcaccccgat ccgccgggcg gcgctctcgc
     2161 cgccgatcat cagcgccgaa tagaagaaga cgaagacgat ggcggcgaag ccgttgaagc
     2221 gggcgaggaa gacgagcggg atcaccgcga ggccataggc cggattccag cccgcctgga
     2281 gattgccgtg cacgcccagg atctcgatgc ccccggcgag tccggcgaag ccggccgaga
     2341 tggcgaagac ggcgaccgtc aaaagcggca cgtcgagccc ggcatggcgg gcggcgagcg
     2401 ggttctcgcc gacggtcctg agcttcaggc cgaaggctgt gcgggtcatc acgaggtgga
     2461 cgacgacgac cgccacgagc ccgagcacca gcccgcccga gatggtcgtg ccgaagagcg
     2521 tcggcagccg gtcggccacc ggcagggtgc gggtctgcgg cacggtggtg ccggggtcga
     2581 ggaagaccag cttgaccaga aggttggcga aggagacgcc gaggaaggtc atcatcagcg
     2641 tggtgatgat ctcgttgacc tgctggtagg cccgcagcag ggccggcagc agcgaccaga
     2701 gagcacccac cagggcagca ccggcggcag ccacgagaag caccgccccg gccggcagga
     2761 cggtgatcag gaagggtgcc agagcagccg aggtcaccgc ccccagcagg aactggccgt
     2821 cgatgccgag attccagatg ccggcgcgaa aggccacgat cagcgccgcc gcgaggcaga
     2881 ggagcggcgc catgcgggtc agggtctgct gcagcccgag ccaggagaag agcccgcgct
     2941 gggcgacata gccgtaatag gcgaagggat cgacgccgag ggcgaggagc acgaggcccg
     3001 agagcacgag ggcggcgagg atcggcccga gcgtcaccag cgccagatgg gcaagcgccc
     3061 ggcgcggccc ggacgtgtcg gccccgcctt catcgagcgt ggtctcgagg ctggcgctca
     3121 tgccgcacgg ccgatcatca gcgccccgat cctcgcccgc gcaccctcgt cattggcgac
     3181 gatgccggtg agccggccgg cggccatgac ggcgatgcgg tcggcgacgt cgagcagctc
     3241 ctcgaggtcg gtggagatca aaagcacggc gaggcccgcg tcggcgatgg cggcgatgcg
     3301 gttgcgcgac gccatgatgt tctgcacgtc gagaccatgg gtcggcttgt tgaagacgac
     3361 gacccgggcg tcgccggaga gctcgcgggc gagcagcacc ttctggatgt tgccgccgga
     3421 gagccggccg atgggcgtgg cctcgtcggg cgtgcgcacg tcgtagcggt cgatcagctc
     3481 gcgcgcgtgg gcggcgatgc gccccggccg ctcgatgccg ccgagcaaaa aggggccgcc
     3541 agaccagaaa ggcggtgccc cgatttcctt cagcaccgca ttggtggcga ccggaaagcc
     3601 tccaaccgcc ccctcgccga tccggtcgtc ggtgaggtag cggagcccga ggcggcggcg
     3661 ctcggccacg cccgcccggg tgatgtcccg gccgtcgagg acgatggagc cggccgccgc
     3721 ggtccgctgg ccggccaagg cctcggcgag ctgcttctgg ccgttgccgt cgatcccggc
     3781 gatgcccagg atctcgcccg gccgcagctc gaacgagacg tcgtccgccc gggcgagccg
     3841 cgttgcccct gtgaccgtga gcccgcgcac gctgaggacc gggggcccct ccgccttgcg
     3901 gagccgggac gggcgggcct cggcgatgct gcggtcgccg aacatgaggc ccaccacctc
     3961 ctcgacggcc gccgcctggt ccatggcggc gagcccctcg ggcgccagct cgccgacctt
     4021 gcggccgagc ctgagcacgg tgatgcggtc gccgaagcgg caggcctcgt cgagcttgtg
     4081 ggtgatgaag acgacggcga ggccgctttt gaccagccgg cgcatgagcg caccgagctc
     4141 gtccgcaccc tgtggcgtca gcatcgaggt cgcctcgtcg aggatcagga cccggctccg
     4201 gcgcagcagt gcccggacga tctcgatctg ctgctgctcg ccgagcgaga gctcgccggt
     4261 cgtctggtcg agatcgagtg ctatgccgaa cgcgcttcgg atcccggcga gacgttgctc
     4321 cagctcggcc cggtcggggc ggcgccacca gctcatgccg aggacgaggt tctccagaac
     4381 ggtgagcgtc ggcaccagca tgacatgctg gaagacggtg ctgatcccct gctgcagggc
     4441 ggccctcggc gaggcgatgc gccgctcctc gccggcgacc cggatcctcc cggcatcggg
     4501 ctcgacgagc ccggagagca tggcgacgag cgtcgacttg ccggccccgt tctcgccgag
     4561 cagcgcgtgc acctcgccgg gcagcaaggc gagatcgacg cggtcgacgg cgagcacgcc
     4621 cggaaagcgc ttggtgacgc cgagcagctc gaccatgggt gcggtggccg ggaccgggcg
     4681 ggctggtgtc gcagcggcct gcatcgtgcc gagcgggacg gacggcgcgg ccactatccg
     4741 tcccgggcct cagatcactc ggccaccgag gtgaccagcg cgcgcacgcc ctcggcgtcg
     4801 aagacgggct ccagcaccag cgtgccgtcg accagctcct ggcgcaccgc catcacctgc
     4861 tcccagacat cggccgggat gttgtcggtc tgcaggagcc gcacgctgtc gtcggcgagg
     4921 ccgatggcgt agctcttggt gccgaacgtg tcgttctcca gatcctcgat catcgccgtg
     4981 tagaccgggg tcatgtccca gagcaccgag gtgaggaggt agcccttgtc gatcgagctc
     5041 ttgtcgccga tcacgtcgat gaacagcacc ttgccgccat cggtcgccgg cgtggtctcg
     5101 atcgcctgca gcatgccgaa gctcgagccg ttgccctggc cgaagacgat gtcggctccc
     5161 gccgcgatca ccgccgcggt gacgcggttg ccgccggcgg cgtccgcata ggcggccggg
     5221 ccgatcacgg catagacgat cttgacgtcc gggttggtcg cctgcacgcc ctgggcgaag
     5281 ccggccgact gcgcgttcca ggacggcggc tcgcccgaga cgacgatgcc gaccgtgccg
     5341 gtgcgggtca tggtggcggc catcacaccg gcgagatagg cgccctcgtg gccggagagc
     5401 gtgtagtcgg cgacgaggcc ggggaccagc gcgtcggggc tgtcgacgat ggcgaccgga
     5461 acaccggtct cctgggcgat ctccggtgcc gccgtgttgt agccgctggc atgggcgatc
     5521 atcaggctcg ccccctcctc cgccagctcg cggagggtcg ggcggatgtc gccatagccc
     5581 aggccctcgg cggtgatcac ctcgaccccg gtggcattgg ccgcggcgac ggcggcatcc
     5641 accccctgct ggttccagcc gtaatccgtg ccttcctcgg gcaccaggat ggcgatcgca
     5701 ttgacttgca cggcgttcgc cacgacagca ccgaaggtcg ccaaggcgag cccgagagcc
     5761 gccccctgca tcagacgctt gatcatgtcc ctctcctttc cgttgccgtt ttctcgttgg
     5821 tcatcgtgcc gaagcgccgt gctctgctgc cttctggcac tcctgcccgc gatgcccgcc
     5881 gccggcagcg agctggtgcc gctggcattc agcgtccgca atctcgccac cgaagcgatc
     5941 acctgtgctg ccaccctggc gcactggttc tcgatcgagc tcggcacggc cgaagccggc
     6001 gagcgcgtgg tggccgagct ctggcacgat cccgatagcg gtgccgtgct gatcctgaac
     6061 gagcagggcg aggccatgcc catcgaggcc ctctggtgcg ggctcgcggg ccggagctgg
     6121 cagacgcgca gccccatccc gctcacccgc aaccccggcg cagcgccagt cggcatcgag
     6181 ctggactgcc ggatggccgg cggccggctc gcctgcaacg acgtgcgccg ctagctcttc
     6241 cgcccggccc cgtggcgagg cgccgctcga cggcggtcgt catccgccgc cgctttcgag
     6301 cacgcggcgc acggcgacca gcgcccgcgc ccgctcggcc ggcaggcgtt ccagctcctc
     6361 cctggtgtag tcgaagagcc cgccgcccgt cttcatgccg agccggcccc tggcggtggc
     6421 ggccgtcacc agcggcgaga cgtcggcgcg gtcggcgagc tccttgttga ggaaggacga
     6481 gaccgcctcg tagatgtcga gcccggccat atcgagcagc cgcatcggcc cgaccaccgc
     6541 gagcttcatg ccgatgcccc agcgcaccac cgtgtccagg ccctcggcat cgatcacccc
     6601 gccctcgacc aggtcgacgc attcgcgcag cagcgcatag agcacccggt tctcgacgaa
     6661 gcccggcacg tccttcttga tgaccaccgg cagcaagccg agcccgtgga tcagaccgcg
     6721 cacggccgcg accgtcccgg gtgcggtggc ggcaccgccg atcacctcga tcatcgggat
     6781 gatgtgcggc gggttggacc agtgcatgcc gacgaagcgc ctggggtgcg ccacatgcgc
     6841 ctgcaggctg gtgatcggga tgcccgaggt gtccgtggca aggatggcgg cctgatcgat
     6901 cagcggctcg acctgacgat agagggccgc cttggtctcg atcttctccg gcacgttctc
     6961 gatgaccaga tccgcaccgg ccaccgtctc ctcgaggctc ccctcgatgc ggatcgcccc
     7021 ctcgcccgcc gtcggcgggg cgatggccag cgcatcgagc acggcggcag cacgggacag
     7081 caagcccgtc gcccgctcgc gcgccgcctc ggcgatgtcg aagccgctga cctcgagccc
     7141 gccccgggcg agccgcgcca tcatgcccgg ccccatggag ccgaggccga tgacggcgac
     7201 cttccggatg gccggggcgg ggctcgccac ggctcagccg ccggtggcgg tcttgaaggc
     7261 cggtctttcg gcgatgccgg ccatccaggc ctcgagcccg acgaagctcg gccggtcgag
     7321 cgggaaatcg aggcagcgct tgacgatggg ggcgagcgcg atgtcggcga cggtcagctt
     7381 gccgagcgcg aaaaacttct tgccggagac gtgccggtcg agcacggcga gattggcgac
     7441 gagatccttc acctgctcgt catagccggc agcacgctcg gccggcggca gcttcgagcc
     7501 cttgaaggcg gccatgtagc ccgggttgat ggcggcgagc aggaagtcca tccagcgctc
     7561 gaccacgctc gcctcggccg gcgtgccgcc ggcgagatcc tttccctggg tggcggccag
     7621 atagcgcagg atcgtgttcg attcccagac caccacctcg ccgtcgacca gtgtgggcac
     7681 cttgccggcc gggttcatgg cgagatactc ggcggtggcg gtattgccga actgccggcc
     7741 gaaatcctcg cgctcgtagg gaatgcccag ctcttccagc atgaagagga tcttctggac
     7801 gttgccggac gtggcccggc cgagcaatct gatcatcgtg ctacctcttg gcttgcagca
     7861 ccgatggccg gcgccgcaga aaaacagaaa tccggaaccg ctcagtcgcc gagcggcttg
     7921 taggcgacgg cgtccatctc gatgcgcgtg gcgatcaccg cccgcgcctc gaccgtggtg
     7981 cgggccagca ccgccccctc gaacatctgg ccgaagacct tgttgtagcg gccgaaatcg
     8041 cgggtatcct cgagataggt gcagacccgg acgacgtcct tcagcgtcgc cccctcctcg
     8101 gccaggatgc gctcgatggc gcggatggtg ccggccgtct cggtttcgat gtcgccggtg
     8161 agcatggtgc cgttctcgtc cttcgagacc tggcccgaga cgaagatgaa gtcgccggcg
     8221 cgcacggccg ggtggaacgg caggttcggg ttcttcttgc cgatggcgta gtgcctgtcc
     8281 gatcggccga ccttgtcggt catgcattcc tccgaaggct caaaaggcgg tggtttcgat
     8341 gatgtccacg ccgtggatgc ggtcgacgag atagacgagg ccgcgctcgt cgatggtgac
     8401 gtcgttggag gagggccggt ccgcgcccgg cggcacgtcc ggcaggaagt ggccgacctc
     8461 gaccggccgg aaggggtccc tgatgtcgac cagccggagc ccctgcgcga accaggcgaa
     8521 cgggatcacc gtgccggcga agcgctcgga cggctggtgg cagccggtca tgggctcctg
     8581 cggcgagccg tcgaattccg gcccctcgac ctggaaggtg gcgatgggtg tcggcagccg
     8641 ctcctcggtg atgtcgtaga tccaggtgaa ggccggggcg gcgggccaga gcttggccac
     8701 gtcctcgtcg gccaccacca tgacccgccg gcccttcagc gtctccggga tcggcaggca
     8761 ggtatgggtc gggtgcggaa agaccgggct cgtgttcacc gccgagacca gcttcgggct
     8821 cgacagctcg ctgatgtcca ggatgaagag cccgtgatgc cagtagctga catagagccg
     8881 gtcgccctgc ctgagcggat ggtggcagcg cggctggggc cattcgcccc agggatactc
     8941 ctcgccgccg gcctgccact gcccctcgac ccaccagcgg ccgacctcct ttgggttcgc
     9001 ggggtcggcg aggtcgagga tgacgacgat attgccgata tagccctcga ccgtcggcga
     9061 gatgtaggca tggcgaccgt cgaaatcgaa gcggtgcacg ccccgtccgc cgcctgacca
     9121 cttgctgagc agacgcggct cggccggccg gctcacgtcg tagacggcga gcccgccgcc
     9181 gaaggccgga tcgcccgcct ggccctggcg ctcgtggttg accagcatca ggccattggc
     9241 gacgcgcacc ttgtgcgagt gccagccggg cggcaggtcg agccgggcga tctggcgcgg
     9301 gctcttcggg tccgacacgt catagaccga ggtgcccgtc ggtgcccgca tgtggccgac
     9361 atagagcgtg gtgccgtcga cccagacctg cccgccgccg ggacagtcga tatgcgagag
     9421 aagctcggtg ttgcggctcc gggccattcc cctcgcccgc tcaggccagc cgggccacgt
     9481 cgccgtcggg cgtcctgccc cacttcttgt agagcgagag cgccttgagc accggctcgt
     9541 cgctggcgac gaaaagatag agcggctcgg tcgccgaggt gttctcgtgg ctgcaccagg
     9601 agccgcccgg caccgccagc gtgtcgaact ggtcccagtc cacccgctgg ccgtcgacga
     9661 tcgaggcccc ctcgccccgg aagggcgaga ccagcaggct cgcggtctgc ttcagggcca
     9721 gggtcttctc gccgggcctg agcatctgca tgaagaaggt catggtcttg aagaccgggc
     9781 cgccgcggaa ggggtcggtg tactcgacca tcagggcctc gtgcgggtcg ccgtcccagt
     9841 ccttgtgctt ctgcaggatc tcctccatcg ccgcccagcg atagacgtac atgggcgagg
     9901 agagcccgcc cccccggtgc tggtcgacga agcgcggcac catcccgccc ttgccgtaga
     9961 cggcctgcga atagtcggcc ggatagcggg ccgactgctc cttcttcttc acgagcttgc
    10021 cgccctcctc ctccatgtag tcgtggtcga agtggatggc gttcagcgtc tcgacgagcg
    10081 gcaggtcgag caccgagagg ttggtcgcct gctggtggcc gacggtgcca tggttgtgcc
    10141 aggcgtcgtt cggggtcagc accatgtcgc ccggcccgaa gacgacgttc tcgccctcga
    10201 ccccggtgaa gttgccggtg cccgtcaggc cgaggcggat ggcattgggc gaatgcttgt
    10261 gcggcggcat gatctcgtcg gcatcgttca accgataggc cgtgtacatg gtcgaaacgg
    10321 ttgcccgtcg tggcgccagg ccgggattga tcaggatcag cgagcggcgc tcgctgtcgt
    10381 tcatggtgat cagctcggcg gcgcgctcga gcagcggcag gatgtcctcc cgccagcgcc
    10441 agagatgcgg aatcgccttc tgcgtcgcca tgagctgctt gatctcgtca tgctcgacgc
    10501 cttccgaggt ggcccagaag gggaacatgc tgcgctcgtg gatctcgtca tagaggtcct
    10561 cgagcgcgtt ggcgcgattg tggacggccg cggctgcatc aaccatattc ttcgcgctcc
    10621 ctccgattgg caaggcaacc acgactgttc agcaaggccg atttggttgc aagtgaattc
    10681 gtcggcggca gcggcacccc gaatcaggtg gcgggatcag gcagcggcgg atgcgagaat
    10741 ccgctcggca gcggcctcgg cgatcatcat caccgcggcg ttgatgttgc ccgacggcag
    10801 gtccggcatg accgaggcgt cgaccacgcg aagcccctcg acgccgtgca ggcgcagctc
    10861 cccgtcgacc accgccccgg cctccggcga cggccccatc cggcaggtgc cgcccggatg
    10921 gtgcacggtg atcgaggtgg cgcggatatg cgcgtcgagc gccgcctcgc tggtcttgcc
    10981 cgcccccggt gcgatctccg cctcgacaaa cgggcgaagc gccgcgcgcc cggccaggtc
    11041 gcgggccatg ccgatgcctt ccctcaggat ccgccactcc cgcgcctcgg aaagaaaatt
    11101 ctgccggatc accggcgccg ccccggggtc cgccgagcgc aaggagaccg ccccccggct
    11161 cgacggccgc accagcacga tgcgcatgac gaagccgtcc ggaaagggcc gcttgaaggg
    11221 ctcgagccag ggccaagcgg cgagcggtgc cgccgtgaag aggagctgca ggtcgggagc
    11281 ggggctctcc gccagcgagc ttcggagaaa cgccaccgtg ccgcccggca cgtcgcccga
    11341 gaagccgtcg ccgccgagcc gggtgcggat caggtccggc acgatccggt ccgcccgcat
    11401 catgtgctgg aacggcccgg gcgacttccg ccggtacatc agcacggccg agacgtggtc
    11461 ctgaaggttc cgcccgacgc cgttgagcgc cgtgtgcacg ggaatgccca aggcggacag
    11521 cgctgcggga tcaccgatgc cggagagctg gagcagttgc gggctgttga tggccccggc
    11581 ggcgagcagc acctcgcgcg ccgcccgcgc ggtgtggccg atctcgccca cgcgataacc
    11641 gacgccggtg gcgcgtttgc cggaaagctc gagcttctgc acatgggcgc ccgtcacgac
    11701 cgtgaggttc ggccgctgca ggaccggacg gagatagccg gtggcggcac tgcagcgcag
    11761 cccgcctttg atggtcatct gcagccgcga aaagccgagc tgatcggcgc cgttgtagtc
    11821 gtcggtccag ccatggcccg cctcccggcc cgccgccgcg aaggccttca ccagctcgtc
    11881 ctgatagcgg cagggccgca ccccgatcgg cccgtcgccg ccgcgccagg catccgcccc
    11941 gccggcccag ctttcctgct tcttgaagta tggcagcacc gcatcgtagg accagtcggg
    12001 caggcccgtc gcggcccagc ggtcgtagtc cgcgcggttg ccccggacat gcgccatggc
    12061 attggtcgac gagcagccgc cgaccacccg gccgcgcgcg cattccaccg cccgcccgtc
    12121 gacgctggcc tcggactcgc agaaatagcc ccagtcgaag agccgcttct gcaggatctt
    12181 gccccagccg agcgggatgt ggatccaggg atgccgatcc cagccccccg cctcgagcag
    12241 cagcacccgg tgccggccgc aggcggtcag gcgattggcg agcacgcagc cggcggagcc
    12301 ggccccgacg atgacatagt cgaactcgct cgtggccacc cgaccgcttc cttcctggct
    12361 cgccgctttc tggcccggct tgccgcttca tggccatgca acgctgccac aacgggcacg
    12421 ggtcaggcaa ccattagccg agtgtggttg tcaatggtcg tgcacctgcc tattctgacg
    12481 gcatgacgac cgaaacgatc atggcgccag gggtgccggc gagcgacgtc gatgtggcgt
    12541 ccatggacct gccgggaacg ctgcccggcg agccggccgg tctcgagcgc atgatggcgg
    12601 cgatcctcga gcgctaccgt gaggcgggcg agctgccgcc ggagcgccag ctccgcgacg
    12661 agctcgccgt cacccgccac cgcctgcgca ccgccctcgc ccggctccgc gccgagggtc
    12721 ggctgccgcc ggcccgcacc ggcagacggc cggtcggcgc cacccaggtc gagggcagtg
    12781 agttcctggc acggctcgcc aacccgctcg agctcatgga gctgcggctg atcatcgagc
    12841 cgaccctggc acggctcacc gcgctgcgcg cgtcgtcgct cgagatcgcc cgcgtcgtga
    12901 agctcgccac cacacccccc ggcagcccgg cggccgccac cgacatcgcc ttccaccgtg
    12961 ccgtggcgca ggcctcgcgc aacacgctcg ccggcgagat ctacgccctg ctccggcgca
    13021 tcggcaccga ccagcgaata cgcgtcgccg gctccgacac ctcctgcccc aggcgcatcc
    13081 gcgagcgcga cggcgagcac cagcgggtcg ccgcggccat cgccgcccgc gacggtgccg
    13141 ccgccgagca ggcgatgcgc gagcatctcc tcgccgtcca gcgccgcatc ctcgagcgca
    13201 ccgccgccgg tggtgcggtc gcctgaacga gcgctgcccg cccggctcag cccgcggtcg
    13261 agcgcgccca gtagtcgatc acgagcacct tctcgcggtg ctggccgacc agcgccacga
    13321 acgcctggtg ggccggatgc ggcaggtagg cctcgcggcc ggcctcgtcg gcgaaggtca
    13381 cgagaaagca gtgggtgaag ccctgcgccc ggccctcgat gctgacatcg gtgccccact
    13441 cgaaatcgat gatctcgggg atctgccccg gcagggccgc gaacgccgcc tcgaccgagg
    13501 cgatctccgg cggtgtcgcc tcgggcttga aggcgaagag cacgatgtgg cgaaggaccc
    13561 tggcgttctg gctcggcatg gcagcgcttc ctcggttcgg tgacgtcacc ccttcctagc
    13621 acggccgcaa gggccggtcc gggccgaatt ccgcgccgcg cccagcgtcc agatttgatg
    13681 cttgatgcat caagaaggcc gttctatcga tcttgcatga tgcgtggcag ctcacaggcg
    13741 gacgctcgcc catgatgacg gccggccagc tccgcgccgc gcgcgctttg ctcgggatcg
    13801 accagcgcac cctggcggag ctcgccgggc tctcggtgcc gaccatccag cgcatggaga
    13861 agagcgacgg cgtggtgcgc ggcaatgtgg attcgctgat gaagctgatc gaggcgctcg
    13921 aggtcgccgg cgtcgccctg atcgcggacg ggcccgacgg cgggcgcggc gtcaggctga
    13981 aggccgcacc tccggcggac gaccaggcgc catgacgacg agcctcgtcc tcattgccac
    14041 cgccctcctc ctcgccacgg cggcactggc cgtggcgcgg gccggccaca agcgcatcca
    14101 cgacatcgtc tacggggcgt gcctcgccct gatgaccgcc atcctcgtca tcgcccttgc
    14161 ggccctcctc accggcagcg gctcggcgct cgtcctgccg ctcggcctgc cctggatcgg
    14221 cgcccgcttc cgcatcgacg ccctcgctgc cttcttcctc gccctcctcg gcgcgggcgg
    14281 cgccttcgcc agcctctacg gccagggcta cggccggcac gaggcggccc ccgagcgcgt
    14341 cctgccgttc taccccctct tcctcgccgg catgacgctc gtcctgctcg ccgacgacgc
    14401 cttcaccttc ctcctcgcct gggaggtcat gtcgctcgcc tcatgggcgc tcgtgctcac
    14461 gcagcaccgc gtcgccgaca atcgccgggc gggctacgtc tatctcgcca tggccagcgc
    14521 cgggaccgtg gccctcctcc tcgccttcgg cctcctcgcc ggggccaccg gcgcctatgg
    14581 cttcgccgag atccgcgatg cgacaccggc cccgctgacc gccaccctcg tgctcctcct
    14641 cgccctcttc ggcgccggct ccaaggccgg cctcgtgccg ctccatgtct ggctgccgct
    14701 ggcccacccg gccgccccca gccacgtctc ggccttgatg agcggggtca tgaccaaggt
    14761 cgcgatctac ggctttttgc gcctcgtcct cgaccttttg ggcgagccgc cctggtgggg
    14821 cgccctcgtc gtcctcttca tgggcggggc caccgcggtg atcggcatcc tcatggccct
    14881 catgcagcgc gacctgaagc ggctcctcgc ctacagcacg atcgagaatg tcggcgtcgt
    14941 cttcaccggc atcggcctcg ccttggcctt cgaggcgagc ggcatggcgg ccgccgccgc
    15001 cctggcgct
//
LOCUS       DAOTFI010000115        14967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4799_length_14967_cov_7.446955, whole genome shotgun sequence.
ACCESSION   DAOTFI010000115 DAOTFI010000000
VERSION     DAOTFI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4799_length_14967_cov_7.446955"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1708
                     /locus_tag="P5558_12880"
     CDS             293..1708
                     /locus_tag="P5558_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25253.1"
                     /translation="MTTIRAIACLAVLALAGCVILPLPIPPTGTAVDSEVAAFDLGST
                     TRAQVHRTFGKPNVLASDHLDVWRLERDPAHLWMLLVIAAGPAGGTETEMRTGGSLSY
                     RVAVAYDADGKMSGWRWSRSRDGQTASSDFAVGGPPFEPLQVVQWNADWVLRSPDGEL
                     LLLDLGSYRRLRVDVVSEIDGTIARSMTGDGRDCGVLVPFNFAGRDVPFGWSGGKLVS
                     VPAALKPDALPCEWGTSAGADFGGVPLDWFPTSRMLFPARLVGELVVLRDPVGTVNIV
                     DLRGRLLASIEGEWSVSHAAANDGSTRLLLRLLAPSGKPAVWLFDRNNRDSFELGKLS
                     AATAADACYPGLPESSPAGRLALAMSSDGKLAALRCSLGVELWSLPVAHEDAERLRIL
                     TLPVDLIPHQLAFSLDGSRLVAAQPGIVVWRASDWQIEAFRRQAGRIGLGVEVDADGS
                     GMATTNGYWRLLPPEVDALLD"
     gene            complement(2023..2226)
                     /locus_tag="P5558_12885"
     CDS             complement(2023..2226)
                     /locus_tag="P5558_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25254.1"
                     /translation="MPIADDLGRSDRHQIIFLSRFRRKRKSRFGSFGNFHFWVCPVAG
                     PAAAMGKIVFWINGLVGPGFVGS"
     gene            complement(2239..3393)
                     /locus_tag="P5558_12890"
     CDS             complement(2239..3393)
                     /locus_tag="P5558_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY25255.1"
                     /translation="MTDPDGPALAVESGGERPVVRLSNQSVPRWAVIGIFLLLLIAGI
                     AYARDFLMPVALAFLLALVFSPVRRFLERRRVPAEASAVLIVGSLLAVLLSGVMLLAA
                     PVQGWIADAPEIVRELEWKTRSLFGGAQAVLEASETVENVTTGGTDPEVQEVVVKEPS
                     MFANLAWVAPAYLAQTVFTLVLLLFLLASGDMFYEKIVHVLPTFSDKRQAMRIARDIE
                     RKLSRYLFTITAINAALGLAVGTAMALLGMPNPLLFGVIAFAFNFVPYIGALAGIAIA
                     LVAGLLAFDEPGVALLPGLAYFALTAIEGQFVTPYFVGRRLEMNTVVIFLSITFWAWL
                     WSAMGMLLAVPLLVTIRAFCEHIPELVPVGKFLAARGSEAEEVGEERTVT"
     gene            3537..4562
                     /locus_tag="P5558_12895"
     CDS             3537..4562
                     /locus_tag="P5558_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25256.1"
                     /translation="MTNTTRRTALALATLALAGLAGGPSPAAAADPVRVGLLSLTSHA
                     PSILAAGKGYFAGEGLDVGFVSFQAAQPMAVAIASGDVDFGVTAISGSLISLAERGVV
                     KVIGGALQETEGVPGQKILASKAAHDAGLATPADLAGKRFGITTAGSSFHYMAHKIAD
                     GAGIPRDSIELVPLNAVPTVIASLKSGQVDAWSIVPNIAGALVKGGEVVEIGSVADYI
                     DDYQVTVIFTSTALVDDRPELVQRFLAGFAKGVDDYNAALVDKTMSEADTAAIVAMIH
                     EYVYTDRPLEAADPAIRAGAMRINDGGRLNLTSVTDQLEWFRSEGLVPETATVETLVD
                     TRFVQTY"
     gene            4582..5352
                     /locus_tag="P5558_12900"
     CDS             4582..5352
                     /locus_tag="P5558_12900"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25257.1"
                     /translation="MELIVEGVSHRYGPTEVLDDISFTIGDGEIVAIIGPSGCGKSTL
                     LRLVGGLERPGEGAVLMRGAPPDRSLNPLTYVFQDFALLPWRTVEGNVSLVLEDHAMT
                     AAERREVIDDVLARTRLAEFKQALPRQLSGGMKQRVAIARALAVRPAVMLMDEPLSAL
                     DSQTRELLLDDLLELWLRERFSACYVTHNLAEAVRLGHRIVVLSRRPGRIRELVRIDI
                     PLAERSTAVAELARLQQELWQLLRDEALAADRELADAG"
     gene            5342..6160
                     /locus_tag="P5558_12905"
     CDS             5342..6160
                     /locus_tag="P5558_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25258.1"
                     /translation="MPAEGPAGDDDDIRPVPFRGGGFRHVPLGYLSPLVFVGLIALWE
                     LGSRTGWISSIALPAPSAVFAAFLDLVETGMLWQHLRASLLRLVVGWTLGTILGIGVG
                     LAIALFSVLRAGLAPVVAALFPVPKIALLPLFIVWFGIGEGSKVATILFGAFFPTVIA
                     TYSGVDNVDRGLIRMGQSFGLAWASIVRKIVLPGAMPAILAGFRISVSISIVLLVAAE
                     MIGARFGIGAYILTAGALFAVEQLIAGVAMLSVIGLTISRLIGRAEKRFLDWRG"
     gene            complement(6271..7179)
                     /locus_tag="P5558_12910"
     CDS             complement(6271..7179)
                     /locus_tag="P5558_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY25259.1"
                     /translation="MLDRRRLARLPLLAPLIAACRPVTVLDTLVPKDGQSVLAAENLS
                     YGPHSRQHFDIYVPTGEAATGGPQPLLLFIYGGGWRSGRKSDYHFAGRAFAARGYVTA
                     VADYRLVPEARYPDFLVDCGRALAAFREVAADYRGDPGWTFVAGHSAGAYNAVMLALA
                     PEIAAAAGYERGSIGAVAAIAGPYDFLPLRVDTTEAAFGEASDKPATQPINRVDATAP
                     PMILLTGTADEVVVPANADRLAAELRAVGVPVEVVRYEGVGHNGALMSIARPFRGYAD
                     ILGDVDAFFERIMAGAPPSRPSAEQS"
     gene            complement(7131..7667)
                     /locus_tag="P5558_12915"
     CDS             complement(7131..7667)
                     /locus_tag="P5558_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25260.1"
                     /translation="MTEIALLLVGFVDLIGLGLVFSIINGLIMTPESPLLPGAASDAA
                     RHLDYGLVIGSFFLALFPGWPQEGAADLPRRCPRRRRAEDPGSLSRQPLPAGARSGGH
                     GGHRGNQPIAPTTMGDARTSATDRDRSMSGRARRRRLAGTGSTSISVDAAPPGPSDCW
                     SPSCSTVDDLRACPSWHP"
     gene            complement(7821..9131)
                     /locus_tag="P5558_12920"
     CDS             complement(7821..9131)
                     /locus_tag="P5558_12920"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRY25261.1"
                     /translation="MHPTMLLDACRVVATRLTMRAANFVVFLILARSLTPAEFGAYGY
                     IVASVLMLSVIADLGLRQATAAAIGEGRDEARLLAQLVLIWLVLGSVAVLAMHLAIQL
                     VGLLDDTALLWTAALVAAPALFMRTWQGIFLGRGQIAALNRSELASRAVLLLGTAVLA
                     AGGWLALQTIVWLLLLSYLVGSLVVLTDVAPRLAAADFRAPAIAIGLLRRGLGFAAGI
                     IAMIMLGRVGVWIVGGLLSADDLGRYFGLMRLAEMVAEIATAVGMVIFSHGVRRTGER
                     AAAVADTLRLTRLVGALMALVSVVILALAEPLLGLALGPAYAGAADAFRLMVIGALAG
                     CYGMMLFPGLSSQGEEKMGALIFIVGTATATFLAFALAASHGLMGAALAMALAQVATL
                     VGFLVAYRRAFGVAVRDALLPRPDDLRQLAAPLLAGLRRRLAGT"
     gene            complement(9239..9679)
                     /locus_tag="P5558_12925"
     CDS             complement(9239..9679)
                     /locus_tag="P5558_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRY25262.1"
                     /translation="MSDYGYALNDIEVGMTASFAKTITDADILMFAGVSGDTNPVHLN
                     EEFACGTPFKGRIAHGMLTASLISTALGTKLPGPGCVYLSQSMKFLAPVRSGDTVRAL
                     VTVREVEHEKRRITLDTVCQVGGKDVLVGEARLLVPARESLEAA"
     gene            9901..10467
                     /locus_tag="P5558_12930"
     CDS             9901..10467
                     /locus_tag="P5558_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRY25263.1"
                     /translation="MQALEAILTRVTVPPARMAAPGPGEAALRRMLAAAAAAPDHGKL
                     RPWRFLVVRGGGRERLGDLFAAGVQAARPDTSPAEIEKQRAAPLRAPVILIAISRADP
                     NHPKIPLWEQEASAACAVQNLLLAAHAMGFAGKWASGGPAAAQAVCTGLGLAPNERII
                     GIIYLGSPMDRQSLPPRPAPEAIAESWP"
     gene            10475..10741
                     /locus_tag="P5558_12935"
     CDS             10475..10741
                     /locus_tag="P5558_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25264.1"
                     /translation="MTPASRLVLAACLALGACEAGPTMEELRTADIAACSAAGFQSGS
                     DAERLCLLLQETNRRLEGVERRMNFLEVDTRRLSGYGRCGPSSC"
     gene            complement(10758..11510)
                     /locus_tag="P5558_12940"
     CDS             complement(10758..11510)
                     /locus_tag="P5558_12940"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRY25265.1"
                     /translation="MSIGIICAIPQELGHLAAALGEVERHIHGGVEFLSGTLDSRAVV
                     LVGGGVGKVNAALTATLLADRFACGTLVFSGVAGGLDPDLAVGDIVIGERTLQHDAGV
                     LQGERTHTYQAGHVPFFNPTDQLGYTVPAPLLQQIRQRLDDFALPPLSPAAGGTGRPP
                     RITFGTILTGDQFLNCEATRQRLFQELGGRAVEMEGGAIGQVAEAFGLPHLVIRALSD
                     LAGAESHFDFGRFVEETAFGSAAILRHLLPVL"
     gene            11569..12417
                     /locus_tag="P5558_12945"
     CDS             11569..12417
                     /locus_tag="P5558_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY25266.1"
                     /translation="MQRAITGLDHLIVGVRDLEAARGIWARLGFNSTPRGRHVGWATA
                     NYCIMFEDDYLELLGIVDPAGFTNGLDERLAAGEGLMGLALGTSDAAQTAAAWRAAGL
                     ASARTAALGRLLESGDEPVELRFETVILDAAERTGLGIFACRHLTPGPMRRPAWLRHP
                     NGARRIAGLTVVADDIEPLRAAAERLVGSAAMSRTDSLWTIQTGTAPVILCRPQDALL
                     LHPDWEPSAGAGPTLHQILEIGVTDLEETARFLALQHVSCRRGQAGALLVEPESATGV
                     DLAFVA"
     gene            12723..13439
                     /locus_tag="P5558_12950"
     CDS             12723..13439
                     /locus_tag="P5558_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY25267.1"
                     /translation="MASILRDADIVPTRPQPSAVLGFLTALPSSPFDYLLLLRFALLN
                     VFGVALLGAAWLNGLLTPVFVADSTGIVVLIAVTFLAGLGLCARRVLQTSIELNAVRS
                     SLPGRDSRVGAHLDRARATDAQGRGNLAAALKLKLATRIAPVRHIANSLVLLGLIGTV
                     LGFIIALSGVDPDLAADIGAVGPMVSTLIEGMAVALYTTLAGSVLSVWLMINYRLLES
                     GVVHLLTQVVERGEAQHGRA"
     gene            13426..14034
                     /locus_tag="P5558_12955"
     CDS             13426..14034
                     /locus_tag="P5558_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25268.1"
                     /translation="MGGLEPFDDDETGTVFRDVILLALAGFVAIVVMILPHLNPPDAR
                     QADAMEPPGNVVVEVRWPDGLDTDIDLWVQAPADIPVGYSNKGGAVFNLLRDDLGKRA
                     DATGLNYEVSYSRGILPGEYTVNVHLYRNPGRTLPVPVTVVTSVKKSAEESARQILAS
                     KVDLTREGQEITAYRFRLDDQGDLVPGSVHNLQRPLRNWKKR"
     gene            14031..14546
                     /locus_tag="P5558_12960"
     CDS             14031..14546
                     /locus_tag="P5558_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25269.1"
                     /translation="MTDVSGFFIVAAVLAFILATHGIWAPRRLGVKISALITTALFLP
                     LAYAATLTLLSKPKPVHLEWWLAQADEATVLGSSMREGDGIYLWLQMTELVEPRAYVL
                     PWDQALAQELQEALRQAEENGSAVQMRLPFEPSLDNRDPKFYALPQPALPPKERLDEG
                     PQMFTGPGREA"
     gene            14619..>14967
                     /locus_tag="P5558_12965"
     CDS             14619..>14967
                     /locus_tag="P5558_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25270.1"
                     /translation="MVAKGIGVLVLHEGADQYLEELHRRFPAVEFRVVREPAGLAAAR
                     GFPASIAYSCVTDGFPRTEHARLLAWPGLDWVHVGGSGFEHLVAGGPPGFLLTNGAGV
                     LAQELAQTLLGALI"
BASE COUNT         2364 a         5165 c         5120 g         2318 t
ORIGIN      
        1 ctgttagccc atggagtggt ttcggcacca ttctcaactc ggacggcacg ggcatggcga
       61 cgaccacagg ttactggcgt ctcctgccgc cgagggttga tacctcgctc gattgaccaa
      121 aggagaagca ataagcgacg caacctggaa agccaccggt cggcaccctt ccccgtcagc
      181 agcgaccggc gctccgagtt caaggggcac accacgcaat cgacctctcg ccagggtcgg
      241 ttgatgcgga tcaatgcgcg gcaccgcatc gcccgtcatg gaccggggca tcatgaccac
      301 gatccgcgcc atcgcctgcc ttgcagtcct cgcactcgcc ggctgcgtga tcctgcccct
      361 gccgatcccg cctaccggca cggcagtgga cagtgaggtc gcggcgttcg atctcggctc
      421 gaccacccgc gcgcaggtgc atcgcacgtt cggcaagccg aatgttctgg ccagcgatca
      481 tctcgacgtc tggcgactcg agcgcgatcc ggcccatctc tggatgctct tggtcatcgc
      541 cgctggccca gccggtggaa ccgagacgga aatgcggacg ggtggatcgc tcagctaccg
      601 tgtggccgtc gcatacgacg ccgacggcaa gatgtcgggc tggcgctggt cgcgatcgcg
      661 ggatggccag acagcgagca gcgatttcgc tgttggtggc ccgccgttcg aaccgcttca
      721 ggtggtgcag tggaatgccg actgggtgct gcggtcgccc gacggggagc ttctcctcct
      781 cgatctcggc agctaccggc gcctcagggt agatgtcgtc tccgaaatcg atggcacgat
      841 tgccagatcc atgactggag atggtcggga ctgcggggtc ctggtgccgt tcaatttcgc
      901 cggtcgggac gtgccattcg gctggtctgg cggcaagctc gtctctgtcc ccgcagccct
      961 gaagcccgac gcgctgccgt gtgagtgggg caccagtgct ggcgcggatt tcggtggtgt
     1021 gccattggac tggtttccga cgagcaggat gctgtttccc gcccgactgg tcggcgagct
     1081 cgtcgtgctt cgcgatcctg tcggcaccgt gaatatcgtc gatctgagag gtcgacttct
     1141 cgcttcgatc gagggcgagt ggtccgtttc acacgccgcg gcgaatgatg gcagcacgcg
     1201 cctccttctg cgactgcttg caccaagtgg aaagccggcg gtttggctgt tcgaccgcaa
     1261 caacagggat tcgttcgagc tcgggaagct gtcggccgcc acggctgccg acgcctgcta
     1321 tccgggactt ccagagtcgt cgcctgccgg caggcttgcc ctggccatgt catccgatgg
     1381 caagctcgcg gcgcttcgtt gctctctggg ggtggagttg tggagccttc cggttgcgca
     1441 cgaggatgcc gagcggctgc ggattctcac cttgcccgtt gatctgatac cccatcagct
     1501 cgcattttca ctcgatggca gccgattggt ggcagcgcag cccggcatcg tcgtctggcg
     1561 cgcctccgat tggcagatcg aagcttttcg cagacaagct ggaaggatcg gcctgggcgt
     1621 cgaagtcgac gcggacggta gtggcatggc gacaaccaat ggctattggc gtctgctgcc
     1681 gccagaggtc gatgccttgc tggattgacg aagcgggaat agctgccaga gggccgtgat
     1741 ctgggaaaag ttgggacagc aacctcgcgg taatctgggg agttgtccgc cgctgttgcg
     1801 ccagatgcaa cgctgtcctc gggttgtcgc gtctggcgcg acattgttat gacaaatgtc
     1861 gcgtctggtg catcattcgc gctcgggatg tcgcattcgg cgctacgttt tccccgggtt
     1921 ggtgcatcag atgcaccact tttcgacgaa ccaacccggc gaaacgacga tcgaacccgg
     1981 gaaaccgacg atcgaacccg gggattcgac gaacgaaccc agctatgagc caacgaaccc
     2041 ggggccaacc aacccgttga tccaaaaaac gattttgccc atcgcggcgg ccggaccggc
     2101 gaccgggcaa acccaaaagt gaaaattgcc gaacgaaccg aagcggctct tgcgtttgcg
     2161 gcggaatctt gataggaata ttatctgatg gcgatcgctg cggccgaggt cgtcagcgat
     2221 gggcatgcga caccgtcctc acgtgaccgt ccgctcttct cccacctcct cggcctcgct
     2281 gccccgggcc gccaggaact tgccgacagg gacgagctcg gggatgtgct cgcagaaggc
     2341 gcggatggtg acgagcagcg gcacggctag cagcatgccc atggccgacc agagccaggc
     2401 ccagaaggtg atcgagagga agatcacgac cgtgttcatc tcgaggcggc ggccgacgaa
     2461 atagggtgtc acgaactggc cctcgatggc ggtcagggcg aagtaggcga ggccgggcag
     2521 gagggccacg ccgggctcgt cgaaggcgag gagaccggct acgagcgcta tggcgatgcc
     2581 ggccaaggca ccgatatagg gcacgaagtt gaaggcgaag gcgatcaccc cgaagagcag
     2641 cgggttcggc atgccgagca gggccatggc ggtgcccacg gcgaggccga gcgccgcgtt
     2701 gatcgccgtg atggtgaaga ggtagcgcga gagcttgcgc tcgatgtcgc gggcgatgcg
     2761 catcgcctgg cgcttgtcgc tgaaggtcgg caggacgtgg acgatcttct cgtagaacat
     2821 gtcgcccgac gccaggagga agagcagcag caccagcgtg aagacggtct gcgccaggta
     2881 ggccggcgcc acccaggcga ggttggcgaa catgctcggc tccttgacca ccacctcctg
     2941 cacctcgggg tccgtgccgc cggtcgtgac gttctcgacc gtctcgctgg cctcgagcac
     3001 cgcctgcgcc ccgccgaaga ggctgcgggt cttccattcg agctcgcgca cgatctccgg
     3061 cgcatccgcg atccagccct gcaccggcgc ggccagcagc atgacgcccg atagcagcac
     3121 ggccagcagc gagccgacga tcagcacggc cgaggcctcc gccggcacgc gccgccgctc
     3181 gaggaagcgc ctgaccgggc tgaagaccag cgccagcagg aaggcgaggg ccaccggcat
     3241 caggaaatcc ctggcatagg cgatgcccgc gatcagcagc agcaggaaga tgccgatcac
     3301 cgcccagcgc ggcaccgact ggttcgacag ccggacgacc ggccgctccc cgccgctctc
     3361 gaccgccagc gcgggcccat cagggtcggt cacggtgcct ccttctcggt atggcggctc
     3421 aaccgtcgga cagggcgtgc ggttccggcg accgggtcat cgagcgggca gggggtcgcc
     3481 acgacttgcc cggacccggc ccttgccaca ataaggggtg atcaagggag gataccatga
     3541 ccaatacgac ccgccgcacg gcacttgccc tcgccacgct cgccctggcc ggcctcgccg
     3601 gcggcccgtc gcccgctgcc gccgccgatc cggtgcgggt cggcctgctc tcgctcacct
     3661 cccacgcacc cagcatcctc gccgccggca agggctattt cgcgggcgag ggcctcgacg
     3721 tcggcttcgt ctccttccag gcggcccagc ccatggccgt ggcgatcgcc tcgggcgatg
     3781 tcgatttcgg cgtcaccgcc atctccggca gcctgatcag cctcgccgag cgcggcgtcg
     3841 tcaaggtgat cggcggtgcc ctgcaggaaa ccgagggtgt gcccggccag aagatcctgg
     3901 ccagcaaggc agcccatgac gccggcctcg ccacgcccgc cgacctcgcc ggcaagcgct
     3961 tcggcatcac cacggcgggc tcctccttcc actacatggc gcacaagatc gccgacggcg
     4021 ccggcattcc ccgcgattcc atcgagctcg tgccgctcaa cgccgtgccg accgtgatcg
     4081 cgtccctgaa gagcggccag gtcgacgcct ggagcatcgt gcccaatatc gcgggtgcgc
     4141 tcgtcaaggg cggcgaggtg gtcgagatcg gcagcgtcgc ggactatatc gacgactacc
     4201 aggtgacggt gatcttcacc tcgacggccc tcgtcgacga tcgcccggag ctggtgcagc
     4261 gatttctcgc gggcttcgcc aaaggcgtgg acgactacaa cgcggcgctg gtcgacaaga
     4321 ccatgagcga ggcggacacg gccgccatcg tcgccatgat ccacgaatac gtctacaccg
     4381 accgtccgct cgaggccgcc gacccggcca tccgcgccgg cgccatgcgg atcaacgacg
     4441 gaggcaggct caacctcacc agcgtcacgg accagctcga atggttcagg tccgagggcc
     4501 tggtgccgga gaccgcgacg gtcgaaaccc tggtcgacac gcggttcgtc cagacgtact
     4561 gagccggttg gtcgcggggc catggagctg atcgtcgagg gcgtcagcca ccgctatggc
     4621 ccgacggagg tcctggacga catctcgttc accataggtg acggcgagat cgtcgccatc
     4681 atcggcccct ccggctgcgg caagtccacg ctgctccgtc tcgtcggcgg gctcgagcgg
     4741 ccgggggagg gggcggtgct catgcgcggt gcgccgcccg accgctcgct caaccccctg
     4801 acctacgtct tccaggactt cgcgctgctg ccctggcgga cggtcgaggg caatgtcagc
     4861 ctggtgctcg aggaccacgc catgacggcc gccgagcggc gggaagtgat cgacgacgtg
     4921 ctggcgcgca cccgcctcgc cgagttcaag caggccctgc cgcgccagct ctcgggcggc
     4981 atgaagcaaa gggtcgccat cgcccgggcg ctcgccgtcc gcccggcggt gatgctgatg
     5041 gacgagccgc tctccgccct cgacagccag accagggagc tgctgctcga cgacctgctc
     5101 gagctctggc tgcgcgagcg gttctcggcc tgctacgtca cccacaacct cgccgaggcg
     5161 gtccggctcg ggcaccggat cgtcgtgctc tcgcgccgtc cggggcggat tcgcgagctg
     5221 gtccggatcg acatcccgct cgccgagcgc agcacggcgg tggccgagct cgcccggctg
     5281 cagcaggagc tctggcagct cctgcgcgac gaggccctgg cagccgatcg cgagctcgcc
     5341 gatgccggct gagggacccg ccggcgacga cgacgacatc cggcccgtgc cgttccgcgg
     5401 cggcgggttt cgccatgtgc cgctgggcta tctctcgccc ttggtcttcg tcggcctcat
     5461 cgcgctttgg gagctcggct cgcgcaccgg ctggatcagc tcaatcgccc tgccggcgcc
     5521 gtccgccgtc ttcgccgcct tcctcgacct ggtcgagacc ggcatgctct ggcagcatct
     5581 gcgggcgagc ctccttcgcc tcgtcgtggg ctggaccctg gggaccatcc tcggcatcgg
     5641 cgtcggtctc gccatcgccc ttttttcagt gctccgcgcc ggcctcgccc ccgtggtcgc
     5701 cgccctcttc ccggtgccga agatagccct cctgccgctc ttcatcgtct ggttcggcat
     5761 cggcgagggc tcgaaggtgg cgaccatcct cttcggtgcc ttcttcccga ccgtgatcgc
     5821 cacctatagc ggcgtcgaca atgtcgaccg cggcctgatc cgcatgggcc agtccttcgg
     5881 cctcgcctgg gcctcgatcg tcaggaagat cgtgctgccc ggcgccatgc cggcgatcct
     5941 cgccgggttc cgcatctccg tctcgatctc gatcgtgctc ctggtcgctg ccgagatgat
     6001 cggcgcgcgg ttcggcatcg gcgcctatat cctgacggcg ggtgcgctct tcgccgtcga
     6061 gcagctgatc gccggtgtcg ccatgctctc ggtcatcggc ctcaccatca gccggctgat
     6121 cggccgggcc gagaagcgct tcctcgactg gcgcggctga acgcgcgcaa ggtgaccgag
     6181 gacctcgtca gcttgcggac gatcggtgct cacgtagctg acgacgccgc tcgcctgggc
     6241 attcctcgtc ttcgctcacc ctgcaaacgc tcaggactgc tcggccgagg gcctcgaggg
     6301 cggcgcgccg gccatgatcc gctcgaagaa ggcgtcgaca tcgcccagga tgtccgcata
     6361 gccgcgaaac gggcgagcga tgctcatcag ggcaccattg tggccgacac cctcgtagcg
     6421 gacgacctcg accggcacgc cgaccgcgcg cagctccgcc gccagccggt cggcattggc
     6481 cggaacgacc acctcgtcgg cggtgccggt cagcaggatc atcggcggag cggtggcgtc
     6541 gacccggttg atcggctgcg tcgccggctt gtccgacgcc tcgccgaacg cggcctcggt
     6601 cgtatcgacc ctgagcggca ggaaatcgta tggccccgcg atcgccgcga ccgcgccgat
     6661 ggagccccgc tcgtagccgg cagccgcggc gatctccggc gccagggcga gcatcacggc
     6721 attgtaggcc ccggccgagt gcccggccac gaacgtccag cccggatcac cgcgatagtc
     6781 cgccgccacc tcgcgaaagg cggcgagcgc ccggccgcaa tcgacgagga aatcgggata
     6841 gcgcgcctcc ggcaccagcc ggtagtccgc cacggccgtc acatagcccc gggccgcgaa
     6901 cgcccgcccg gcgaaatggt agtccgattt ccgtccgctg cgccagccac cgccatagat
     6961 gaagagcagg agaggctgcg gcccgccggt ggctgcctcg ccggtcggca catagatgtc
     7021 gaaatgctgg cgggaatgcg gtccatagga gaggttttcc gctgccagca ccgactggcc
     7081 atccttcggc accagcgtgt cgagcacggt gaccggccgg caggcggcga tcaggggtgc
     7141 caggaggggc aggcgcgcaa gtcgtcgacg gtcgagcaag atgggctcca gcagtcagat
     7201 gggccgggcg gggccgcatc aaccgagata gaagtcgatc cggtgccggc cagtcgccgc
     7261 cgccgggcgc ggccgctcat gctgcggtcg cgatccgtcg cgctggtgcg ggcgtcgccc
     7321 atcgtggtcg gggcgatcgg ctggttgccg cggtgacccc cgtgaccgcc cgaccgggca
     7381 ccagcaggca gaggctgccg agatagagag cccggatcct cagcgcgtcg tcgacgaggg
     7441 catcgccgag gcagatcagc agcgccttct tgcggccacc cagggaacaa ggcgaggaag
     7501 aagctcccga tcaccagccc gtagtcgaga tgccgcgccg cgtccgatgc cgcgcccggc
     7561 agcagcggcg attcgggcgt catgatcagg ccgttgatga tcgaaaacac caggcccagg
     7621 ccgatcaggt cgacgaagcc gacgaggagc agcgcgatct cggtcatgat gggcatgctc
     7681 ggtcgccccc ggcttgcctg gaccttgcgc cacgctcgca gatgcgccgc acccggccgg
     7741 gatggcgagc cggccggaac gccggctgcc tgacccgtgg atcggtggcg cggtggggcc
     7801 cggctgcctg tcggccggga tcaggtgccg gcgagccggc gcctgaggcc ggcgaggagc
     7861 ggggcggcga gctggcggag atcgtcgggg cgcggcagta gcgcatcccg gacggcgacg
     7921 ccgaaggccc ggcgataggc cacgaggaag ccgacgaggg ttgccacctg ggcgagagcc
     7981 atcgcaagtg cggcacccat caggccgtgg ctcgcggcga gggcgaaagc gagaaatgta
     8041 gccgtggcgg tgccgacgat gaagatcagg gcacccatct tctcctcgcc ctgcgacgag
     8101 aggccgggaa agagcatcat gccgtagcag ccggcgagcg ccccgatgac catgagccgg
     8161 aaggcatcgg cagcaccggc ataggccggg ccgagcgcca gccccagcaa gggctcggcg
     8221 agggccagga tgaccaccga gacgagcgcc atgagggcgc cgacgagccg ggtcaggcgc
     8281 agcgtgtcgg ccaccgccgc agcccgctcg ccggtgcggc gcacgccgtg gctgaagatc
     8341 accatgccga cggccgtggc gatctcggcg accatctcgg cgaggcgcat gaggccgaaa
     8401 tagcggccga gatcatcggc gctcaggagg ccgccgacga tccagactcc cacccggccg
     8461 agcatgatca tggcgatgat gccggcggcg aagccgaggc cacggcggag caggccaatg
     8521 gcgatggcgg gtgcgcggaa atcagcggcg gccagccggg gcgcgacatc ggtgagcacc
     8581 acgagcgagc cgaccagata ggagaggagc agcaaccaga cgatggtctg gagggccagc
     8641 cagccgcctg ccgcgagaac ggccgtgccc aggagcagca cggcgcgcga ggcgagctcg
     8701 gagcggttca gggccgcgat ctgcccgcgg ccgaggaaga tgccctgcca cgtgcgcatg
     8761 aagagggcag gggcggcgac gagagcggcg gtccagagga gggccgtgtc gtcgagcagg
     8821 ccgacgagtt ggatcgccag atgcatggcg agcacggcaa cgctgccgag caccagccag
     8881 atcagcacga gctgcgcgag aagccgtgcc tcgtcacgac cctcgccgat ggcggcggcg
     8941 gtcgcctggc gcaggccgag gtcggcgatc accgagagca tgagcaccga ggcgacgatg
     9001 tagccatagg caccgaactc ggcgggcgtc aggctgcgtg ccaggatgag gaagacgacg
     9061 aaattcgcgg cgcgcatggt gagccgggtc gccacgaccc ggcacgcatc gagcagcatc
     9121 gtggggtgca tggacccgcc ccgtggttcg acccgccgcc cgccgatcgg gcgacccgcc
     9181 tacactggca gccccgcctt aaatcgaggt taaggcgggc ccgtgtcgga ggctttcgtc
     9241 aggcggcttc gaggctttca cgcgccggca ccagcaggcg ggcctcgccg accagcacgt
     9301 ccttgccgcc gacctggcag accgtgtcca gggtgatgcg ccgcttctcg tgctcgacct
     9361 cgcgcacggt gacgagtgct cgcaccgtgt cgccggaccg caccggggcc aggaacttca
     9421 tcgactggct gagatagacg cagcccggac ccggcagctt ggtgccgagc gcggtggaga
     9481 tcaggctcgc ggtgagcatg ccgtgggcga tgcggccctt gaacggcgtg ccgcaggcga
     9541 actcctcgtt caggtggacc gggttggtat cgccggagac gccggcgaac atcaggatgt
     9601 cggcatcggt gatggtcttg gcgaacgagg ccgtcatgcc gacctcgatg tcgttcagcg
     9661 cgtaaccgta gtcggacatg tcgtcgttcc ttttggccgc tcgcgagccg agcgggcggt
     9721 tggtcccgag cgggaccgct gctgcgggcg ccattcgtgc gttgcagcat gcccatgcgg
     9781 ctctgcagca tcaagagaca atcctatctt ccgcaccacg cattgaatcc gatcccggtt
     9841 aagaacccgt tgacgatgca ggtacggcga tcgacatcag gcccgatcga aggagacatc
     9901 atgcaggcac tcgaggcgat tctcacccgg gtcacggttc cgccggccag gatggcagcc
     9961 cccggcccgg gcgaagcggc actgcgccgc atgctggcgg cggcggcggc ggcacccgat
    10021 cacggcaagc tgcggccctg gcggttcctc gtcgttcggg gcggcggccg cgagcggctt
    10081 ggcgacctct tcgccgccgg cgtgcaggcg gcgcgccccg acaccagccc ggccgaaatc
    10141 gagaagcagc gggccgcacc gttgcgcgcg ccggtgatcc tgatcgccat cagccgggcg
    10201 gacccgaacc atccgaagat cccgctctgg gagcaggagg cctcggcggc ctgcgccgtg
    10261 cagaacctgc tgctcgcggc gcacgccatg ggcttcgccg gcaagtgggc gagcggcggt
    10321 ccggctgccg cccaggcagt ctgcacgggc cttgggctgg cgccgaacga gcgcatcatc
    10381 ggcatcatct atctcggcag ccccatggat cggcagagcc tgccgccccg cccggcgccg
    10441 gaggcgatcg ccgagagctg gccgtgacgg cgccatgacg ccggcgtcac ggctggttct
    10501 cgctgcctgc ctcgcccttg gtgcctgcga ggcagggccg accatggagg agctgcgaac
    10561 ggccgacatc gccgcctgct cggctgccgg gtttcagtcc ggcagcgacg ccgagcgcct
    10621 ctgcctgctc ctgcaggaga ccaatcggcg gctggagggt gtggagcggc ggatgaactt
    10681 cctcgaggtc gacacgcgcc gcttgagcgg ctacggccgt tgcgggccca gctcgtgctg
    10741 accgccagcg ggcgggatca gaggaccggc agcaggtgac gcaggatcgc cgccgagccg
    10801 aaagcggttt cctcgacgaa gcggccgaaa tcgaagtgcg attcggcacc ggcgagatcg
    10861 gagagggcgc ggatcacgag atgcggcagg ccgaacgcct cggcgacctg gccaatggcg
    10921 ccgccctcca tctcgaccgc ccggccgccg agctcctgaa agaggcgctg ccgcgtcgcc
    10981 tcgcaattga ggaactggtc gcccgtgagg atcgtgccaa aggtgatcct gggcggccgg
    11041 cccgtgccgc ccgcggccgg cgagagcggc ggcaaagcga agtcgtcgag ccgctggcgg
    11101 atttgctgga gaagcggcgc cggcaccgtg tagccgagct ggtcggtcgg gttgaagaag
    11161 gggacgtggc ccgcctgata ggtgtgggtc cgttcgccct ggagcacgcc ggcatcgtgc
    11221 tgcagcgtgc gctcgccgat gacgatgtcg ccgaccgcga ggtccgggtc gagcccgccg
    11281 gccacgcccg agaacacaag cgtgccacag gcgaaacggt cggcgagcag ggtggcggtc
    11341 agcgccgcgt tgaccttgcc gacgccgcca ccgaccagca ccacggcccg gctatccaac
    11401 gtgccggaca ggaattcgac gccaccatgt atatgccgct cgacctcgcc gagcgctgcc
    11461 gccagatggc cgagctcctg ggggatcgcg cagatgatgc cgatgctcat cggtcctcct
    11521 caatggtcca gcgcgtgtcg gcaaggcggt agcgaaagga ccgaagccat gcaacgcgcg
    11581 atcaccgggc tcgaccacct gatcgtcggc gtgcgcgacc tcgaggcggc acgtggcatc
    11641 tgggcgcggc tcggcttcaa ctctacgccc cgcggccggc atgtcggctg ggcgaccgcc
    11701 aattactgca tcatgttcga ggacgactat ctggagttgc tcggcatcgt cgatcccgcc
    11761 ggcttcacca acgggctcga cgagcgattg gcagcgggcg aggggctcat gggcctcgcc
    11821 ctcggcacca gcgacgcggc gcagaccgcg gcggcctggc gggctgccgg tctcgcctcg
    11881 gcgcgcaccg ccgcgctcgg ccggctgctc gagagtggcg acgagccggt cgagcttcgc
    11941 ttcgagaccg tgatcctgga tgcggcggaa cggaccgggc tcggcatctt cgcctgccgc
    12001 cacctcacgc ccgggccgat gcgccggccg gcctggctcc ggcatcccaa cggcgccagg
    12061 cggatcgccg ggctcaccgt cgtcgctgac gacatcgagc cgctccgggc ggcggctgag
    12121 cggctcgtcg gtagtgcggc aatgagccgc accgactcgc tctggaccat ccagacgggc
    12181 acagccccgg tgatcctctg ccgcccgcag gacgcgctgc tgctgcatcc cgactgggag
    12241 ccgtcggccg gagcaggccc gacgctccat cagatcctcg agatcggcgt caccgatctc
    12301 gaggagaccg cacgttttct tgcgctgcag catgttagct gccgccgcgg gcaggctggc
    12361 gcgctgctcg tcgagccgga atcggcaact ggtgtcgatc tcgccttcgt cgcctgacct
    12421 cgcccggacg agcattctac atctgccgac gagtattctg ggccggcatg cgaagggcga
    12481 cattgcaagt attttagctg aatcgaaatc aattcaaggt agaataagat agggcttttt
    12541 gttacgttcc tgggtagcgg ggcgtgatcg gttaagggcc tgttaaccaa gcttccggca
    12601 acatccatcg atcgtgcctg tctggtgacc ttcgccgcac gctttcgtct cgcgagtgcg
    12661 gcgaattcgt ccggccgccg cggcatcgtc gcggccgcca tcctcgagct ggagtgggtt
    12721 ccatggcctc gatactcagg gatgccgaca tcgtaccgac caggccgcaa ccgtccgcgg
    12781 tcctgggttt cctcacggcg ctaccgtcct cgcctttcga ctacctgctg ctcctgcgct
    12841 tcgcgctgct caacgtcttc ggcgtggcgc tcctgggcgc tgcctggctc aatggcctgc
    12901 tgacaccggt ctttgtcgcc gattccaccg gcatcgtcgt cctgatcgcc gtcaccttcc
    12961 tcgccggcct cggactctgc gcccgccgcg tcctgcagac cagcatcgag ctcaacgcgg
    13021 tgcgcagcag cctgccgggg cgggactcgc gggtcggcgc ccatctcgac cgggcgcggg
    13081 cgaccgatgc ccagggccgc ggcaacctcg ccgccgccct caagctcaag ctcgccaccc
    13141 ggatcgcacc ggtcaggcac atcgccaaca gcctcgtgct gctcggcctg atcggcaccg
    13201 tgctcggctt catcatcgcc ctctcgggcg tcgatcccga cctcgcggcc gatatcggcg
    13261 ccgtcggccc catggtctcg acgctgatcg agggcatggc cgtggccctc tacacgaccc
    13321 tcgccggctc ggtcctgagc gtctggctga tgatcaacta ccgcctcctg gaatcgggtg
    13381 tcgtccatct gctcacccag gtggtcgagc gcggcgaggc ccagcatggg cgggcttgaa
    13441 cccttcgatg acgacgagac ggggacggtc tttcgcgacg tcatcctcct cgccctcgcc
    13501 gggttcgtcg ccatcgtggt gatgatcctg ccgcatctca acccgcccga cgcccgccag
    13561 gcggacgcga tggagccgcc cggcaacgtc gtcgtcgagg tgcgctggcc ggacggcctc
    13621 gacaccgaca tcgacctctg ggtgcaggcc ccggccgaca tcccggtcgg ctactcgaac
    13681 aagggcggtg cggtcttcaa ccttctgcgc gacgatctcg gcaagcgcgc cgatgctacc
    13741 gggctcaact acgaggtgag ctactcgcgg ggcatcctgc cgggcgagta cacggtcaac
    13801 gtccacctct accgcaaccc cgggcgcacc ttgccggtgc ccgtcaccgt cgtcaccagc
    13861 gtcaagaaga gtgccgagga atcggcgcgg cagatcctgg ccagcaaggt cgacctcacc
    13921 cgcgagggcc aggagatcac cgcctatcgc ttccggctcg acgaccaggg cgacctggtc
    13981 ccgggcagtg tccacaacct ccagcgcccc ttgcgcaact ggaagaaacg atgaccgacg
    14041 tctcgggctt cttcatcgtc gccgcggtgc tggccttcat cctggcgacg cacggcatct
    14101 gggcaccacg ccggttgggg gtgaagatct cggccttgat caccaccgcc ctgttcctgc
    14161 cgctggccta tgcggcgacg ctgaccctcc tgagcaagcc caagccggtc cacctcgaat
    14221 ggtggctggc gcaggcggac gaggcgacgg tgctcggcag ctcgatgcgc gagggcgacg
    14281 gcatctatct ctggctgcag atgaccgagc tggtcgagcc gcgcgcctat gtcctgccct
    14341 gggaccaggc gctggcgcag gagctgcagg aggcgctgcg ccaggccgag gagaacggca
    14401 gcgccgtgca gatgcgcctg ccgttcgagc ccagcctcga caaccgtgac cccaagttct
    14461 acgcgctgcc gcagccggcc ttgccgccga aggagcggct ggacgagggg ccgcagatgt
    14521 tcaccggccc cggccgggag gcctgagccg cttgacgcag ccgtcgccgg ccgtctttgc
    14581 tccgggcctt gccggggttc ggcgggagga ccagcgacgt ggtggccaag gggatcggcg
    14641 tgctcgtgct gcacgagggc gccgatcagt atctggagga gctgcaccgc cggtttccgg
    14701 ccgtcgagtt tcgggtcgtg cgcgagccgg ccggtctcgc ggccgccagg ggcttccccg
    14761 cctccatcgc ctatagctgc gtcaccgacg gttttccccg caccgagcat gcccggctcc
    14821 tggcctggcc gggcctcgac tgggtgcatg tcggcggctc cggcttcgag catctcgtcg
    14881 ccggcgggcc gcccggcttc ctcctcacca atggcgccgg cgtgctggca caggagctcg
    14941 cccagaccct gctcggcgcc ctcatcg
//
LOCUS       DAOTFI010000116        14823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4855_length_14823_cov_7.872969, whole genome shotgun sequence.
ACCESSION   DAOTFI010000116 DAOTFI010000000
VERSION     DAOTFI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4855_length_14823_cov_7.872969"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3604)
                     /locus_tag="P5558_12970"
     CDS             complement(<1..3604)
                     /locus_tag="P5558_12970"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRY25271.1"
                     /translation="MLRKILLSLLGIVLGTVVLFGAALALAQTRVAKDQISGLIESSL
                     EGEQQTAEVEDLSGFLPFDIRIGRFSLADDEGRWLEVDNARVKLAPTPLLRGEVVIEE
                     AGAARVAVQRAPNLPPPPEPPPAPETDEPFSLPAAPELPESLPRVTAERIHVDRIELG
                     QALIGQDAVFNLEGNATTGPEGRHAEARLDLTRIDQATASLGLAATLDLAQRRIGLDV
                     EGSETGGLMASLTGIGQAGDLTLQLKGEGPLDNWRADLALAVQGLVAADAGLALAYGE
                     NPSIDLQAEVVPVEGAMPADIAAVLGDRLTLAVVGGQRAPGQFVLDRLRLESGIATAE
                     GRLEALLDDNRLDGSIRVAADDLGRASGLAGTPLAGRVELVLDALGTFSEPRFRLDLD
                     GGGITADAFGVETLGLEITADLLGPLDQPFAGARVEGSGEIAGITQDGEPLRPEDRLT
                     LDLALTVPMEGEAVVERLALHGQHVAVDGTASLMMPGLGGTARLEANVPSLEALVAAL
                     GPAAPPDLAVAGAFNLRADIEMVDELKRITLDLVGAGTGIAGLPQNADALVGADPRLT
                     SHVVVVPGSSVSVESLELTTAALGLTGNLDLGLDEAQALSGRVDLSPLALATLEGLVG
                     QPIAGELTSFVTLGGTLPAPEVAADVTVVGLAIAERQFDRVALDARTSGPLAEPAGEL
                     TLAIEQGTDSLALKSDFALDGQSLDLANLSLTAPFTELTGAATVALDGPLATGQLAGR
                     VGDLAALEPWTGQVLSGAIDLDARFDGSAGRQDATLSLTAADVGGDFGNLERASVEAK
                     VEDALGRLGVDATVEAGGFTQPEPGFVLEGATVTVKGDRSLFNLDARANGQMNGPFKV
                     TSKARLDVLGETREVYLDELDGVFQAQSIELLSPAKLRLENGVLDLDQLDLKIGDASI
                     RGGLNFDQPANRARAGLVIKSFPMTMLADFGAPGLEGELTGEVDLDGPLGAPVITGNI
                     AIAGLAYSDGYDTVGRPADIRLDLALRDARFTADATVDGLGDGPITAEFRLPVRLSLE
                     PFAFELPETLPLDGRLAANSRLAPLVAFAHLDGQQLDGQLAADLRLRGTVARPEVEGE
                     LSITGGSFSDAISGIVLTDLALTIEGQGDRLELVSLSARDRANGRLDASGGVGIDPDT
                     GFPYDFTLASTDLRVLDSDLGRATVTVSLEAKGDAEGGALSGKVEVPRADLRLPE"
     gene            complement(3722..5647)
                     /locus_tag="P5558_12975"
     CDS             complement(3722..5647)
                     /locus_tag="P5558_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HRY25272.1"
                     /translation="MAMPIGRRAQGAARGRSAGAGLRRFCRLLAVVLLPALAACSAGN
                     GGGLGDDESQSGPRITYDVRFEGPADSTLVGQLRAASTAAQETGRPPPSLAILRNRAR
                     GDVPRLEATLRSEGYYEGAVGFRIDEDPAPPTLATGVGMVDQALGAVAAHVVFEVEPG
                     PLYHFLDRKIEVIGDPHGYEAPSPGKIGLREGEPARADVVIAAESSLLRDARVQGHAM
                     ARVGDRRSIIDPETRTMDVELAIEPGPVFHFATPVVFGQTDIDDGFLEQRTKIVPGRR
                     YDPADVERAQQRLIDTNLFAAVRVIEGPEPDADGNWPVSFEVTERKPRTIGAGVGYVS
                     DEGPNVRAFWEHRNILGAAERLRVEAELGQSVQRLQGQLRKPDIFMPDLDLLAGGSLS
                     HEDTDAFESTSIQASLGVERHFNKRLSGTLGVAYRLADVEDQDNEEERFALLSIPAGL
                     RYDRSDSLLDPSTGYRIVLETAPYWDTLNPGTQFLKSQLTGTRYFRLAKNPRLVLAFR
                     GIAGSIVGASINEIPADERFYAGGGGTVRGVPFQRAGPLDDGEPTGGRSLLAANAEVR
                     YRVVGNLEGVVFLDGGSAFEPQTPELNEAWQFGTGAGVRYISPVGPIRADVGVPVDRR
                     KGIDDAWQLYISIGQAF"
     gene            complement(5647..8019)
                     /locus_tag="P5558_12980"
     CDS             complement(5647..8019)
                     /locus_tag="P5558_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRY25273.1"
                     /translation="MTFRRLRPWMAALLGVLALWALAGPAHAQMMPMSPAAPAAADTS
                     ASDDIEAVIRTLEDPERRAALLDRLRAAREGEAPPSAKPPSDTVAVTVVEDLTTELRR
                     RSANLAEILTDAIESTEQLPALYEWVEDQVTDPEQRTVWVGVLGAVVLILAVGLAARS
                     MVLRLRPEPDPARPKATLLGRFAIEIVAAVTFAGLTLGMLWISNSVAAIYDVDIQRVA
                     GAELSLGGLLFVAMLWGAGVRLLFGETGIGLHLASVGDGTAAICRRGLLGIGRLGLIG
                     FGVLYALLVLGLPEPLFTFLVHLLYLAVAGIAIVLIVRVREPVAEGIRTWNENSQSPL
                     VRFIPGGFLARTWSYFAIGLVLLHYLVWALKVPNGIIFLSRATVSTFAILVVARLATL
                     GIKRLLPDGAPAAGDSEDALPGVQERADRYAGPARLLLRGAVSLAAMIAILSVWNTGV
                     LDWLTSPTGRDFLALVGSIMLIVASTIAAFELTGYLANRFANAKDQFGKPRHSNRSRT
                     LASIFKNVAFFFFGMAGLFTALGHLGVEAAPLLAGAGVVGLAIGFGSQALVKDLITGL
                     FILLGDTIRVGDVIDIAGKAGVVENMSMRTITLRSYDGNAHTIPYGSIDVVTNMTKEF
                     SFAVMDVGVAYRENTDEVIRVLREIDDRMRKEWPYRRLIVEPLDIAGVDALGDSAVVI
                     RMRSKTRPGEQWGIRREFLRRIKLRFDELGIEIPFPHQTVYFGVGKDGRAPPMFIERA
                     HHALRADEAAAEPPADDEAAGPAAAEATAAARPSLSIAAADTGQRRRGGT"
     gene            complement(8167..9189)
                     /gene="galE"
                     /locus_tag="P5558_12985"
     CDS             complement(8167..9189)
                     /gene="galE"
                     /locus_tag="P5558_12985"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRY25274.1"
                     /translation="MPEAPTGLRVLVTGGAGYVGSHIVRDLIAAGHRVTVFDNLYSGH
                     EWAVPGAELEIGDLGDRQRLERVLSAASFDAAIHCAGHIWVGESVRHPAKYYFNNASN
                     AFRFFDHCAQYGIRHVVFSSTAAVYGQPDVELIDEDQPLRPINPYGASKLMAERALQD
                     IAAAAGLGYAILRYFNVAGADAAARIGEATPNNSHLVKVALETAIGLRPAMHVNGTDY
                     PTPDGTCVRDYVHVDDLAAAHVLALHHLVGGGEPLIANCGYGHGFSVREVIEAVRRVT
                     GIDIPVQSGPRRPGDPAVLVADNRRLCRTLGWRPAGNSLDAIVGSAWRFEQALQSLRG
                     GLAKTG"
     gene            complement(9372..10094)
                     /locus_tag="P5558_12990"
     CDS             complement(9372..10094)
                     /locus_tag="P5558_12990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRY25275.1"
                     /translation="MPQGARVYAVGDVHGRIDLLRALEAQIVADAASRAERCRNLLVY
                     LGDYVDRGFDSRKVLDHLASEPPDDFDRVLLLGNHDAWLRDFLAGEPVGESWLRFGGD
                     ATLLSYGVPLALDRSPEERLSEARDRLQTEVPATHRRLLVDLELALPFGDYLFCHAGI
                     RPGVPLADQVEEDLIWIREPFLSWVGDAGKIIVHGHTVEDAPAIRRNRIGIDTGACYT
                     GNLTCVVLEGTDHRFLSTGQPR"
     gene            10571..11803
                     /locus_tag="P5558_12995"
     CDS             10571..11803
                     /locus_tag="P5558_12995"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRY25276.1"
                     /translation="MTTTRLIGALVAGAAVLAPLATAAQDIRNVPVQNRPRPDYDPLG
                     IRARAFLIYPEIETGVAYDDNIFSTSDNEDDDVIFTVRPAITARSQWSRHQLNLSAFG
                     DFGFYADHNDSNYQDFGVGAGGRIDALRNSRTNIQTNFARLHEDSEEADAQGNEDVTQ
                     YYRMGINLSHRHEFTRVYVEPIARFLRLDYDNAGDVSNTDRDRNEYALGLRTGYALSP
                     RIAPFFELTGSAREYDETPGLDRNSTGFDALIGTTIDFTGVIFGEVSAGYTRREYDDN
                     DLENVDGLAGNAAVTWNVTPLTSVIFDGQAGIDETTITYEGDTATANNYAGLAINVFH
                     ELRRNIIVNVNGEYVRDDYEGTGRTDNTYRAGAGVTYLINRRFSLDATYDFSHRQSDD
                     GDAEFDRNIFRIGLSAKL"
     gene            11984..12595
                     /locus_tag="P5558_13000"
     CDS             11984..12595
                     /locus_tag="P5558_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRY25277.1"
                     /translation="MMTTTCRKLLAGLVLGLGLLLSACGSPSPGTLGPAAGAGTGADG
                     TPAARLSAPAYTLDSGDRIRIVVFRHEDLSGEFEIDGGGTFAMPLIGEINAAGLTARQ
                     LEDRIATRLKDGYLVDPQVSIEVLNYRPFFILGEVNAPGSYPYVSGMSVINAVALAGG
                     FTYRAKEDGIEIQRGGSGAPSVVVTVDTPILPGDILRVPERFF"
     gene            12691..14607
                     /locus_tag="P5558_13005"
     CDS             12691..14607
                     /locus_tag="P5558_13005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY25278.1"
                     /translation="MAEARQGKPGWLDWRSWLASLAIYRDPRVVGVLFLGFSSGLPIM
                     LIAATLSTWLREEEVTRAAIGLFGFAFAPYTFKFAWAPLMDRLPLPPFTSLLGRRRGW
                     MIAAQIGLVLSIFALGRTSPAEDLWWTAFFAVLVAFFSASQDIVIDAYRVEILPKEKL
                     GAGAGVVVLGYRVAMWVATAGALLIAEAFGWSAAYTVMALLVLVGMGTVLSIGEPAQS
                     STALIDQEVLEEDRLLRQTTPARLAPFKGLGGLLLAAVIGLGVWQPLSTLFGIVTAPP
                     ESWSISGAIVLAGNLAVTALGMRLAWLLYRRAPDALARTKNFALLAIAWATVETLWYA
                     IGPEAPFFATLHGWLVLGLLFLVSNWVVGLFGAAPFTYEAFMAPVWLTVVGFWLELVA
                     LLTLYGFTYFSRRAQANFGMVPIAEESRVHLWMRRAVIEPFYDFVRRNGLRVAVVILA
                     FISVYKAADVVLTLMANPFYIDVGFDKAEIAWVSGTFGLWMTLIGGILGGVLVFRLGL
                     MLSLALSVALMAGSNLMFLLLSWVGPNLALFHAVILVENVTAGLGTTVFVAYLSSLCN
                     IHYTAVQYALLTSFMQLLGKFVIVPSSGFFADAVGWSVFFLTSTLFALPALFLLWLLW
                     RWGLAVETPAAARA"
     gene            complement(14635..>14823)
                     /locus_tag="P5558_13010"
     CDS             complement(14635..>14823)
                     /locus_tag="P5558_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY25279.1"
                     /translation="ADPALPIVMVTHHSTQDVVVKALRQGAFGFIVKPYSLSRLKEIF
                     GKLERREADARSMQEVPA"
BASE COUNT         2242 a         5208 c         5137 g         2236 t
ORIGIN      
        1 cctccggcag gcgcaggtcg gcacgcggca cctcgacctt gccactgagt gctccgccct
       61 ccgcatcgcc cttcgcctcg agcgagacgg tgaccgtggc ccggccgaga tcgctgtcga
      121 ggacccgaag atcggtgctg gccagggtga agtcgtaggg aaagccggtg tcgggatcga
      181 tgccgacacc gccgctcgca tcgagccggc cgttggcgcg atcccgggca ctcagcgaga
      241 ccagctcgag acggtcgccc tggccctcga tggtgagggc cagatcggtg aggacgatgc
      301 cgctgatcgc atcgctgaag ctgccgccgg tgatggagag ctcgccctcg acctcggggc
      361 gggcgacggt gccccgcagc ctgaggtcgg ccgcgagctg gccgtcgagc tgctgcccgt
      421 cgagatgggc gaaggcgacg agcggcgcca gccggctgtt ggcggcgagc cggccgtcga
      481 gcggcagcgt ctccggcagc tcgaaggcga agggctcgag cgacagccgc accggcagcc
      541 ggaactcggc cgtgatcgga ccgtcgccga gcccgtcgac ggtcgcatcc gcggtgaagc
      601 gtgcgtcgcg caaggcgagg tcgaggcgga tatccgccgg ccgaccgacg gtgtcgtagc
      661 cgtcgctgta cgcgagtccg gcaatggcga tgttgccggt gatgaccggg gcaccgagcg
      721 ggccatcgag gtcgacctcg ccggtgagct cgccctccag cccgggtgcg ccgaaatccg
      781 ccagcatcgt catggggaag ctcttgatga cgaggcccgc acgggcgcgg ttggccggct
      841 ggtcgaaatt cagcccgcct cgaatactgg cgtcgccgat cttcaggtcg agctggtcga
      901 gatccagcac gccattctcg agccgcagct tggccgggct caagagctcg atgctctggg
      961 cctggaaaac gccgtcgagc tcgtcgagat agacttcccg ggtctcgccc agcacatcga
     1021 gccgggcctt gctcgtcacc ttgaacgggc cgttcatctg accattggcc ctggcgtcga
     1081 ggttgaagag cgaccggtcg cccttgaccg tgacggtggc accctcgagg acgaagcccg
     1141 gctccggctg ggtgaagccg ccggcctcga ccgtcgcatc gaccccgagc cggcccagcg
     1201 cgtcctcgac cttcgcctcg acggacgccc gctcgagatt gccgaaatcc ccgccgacgt
     1261 cggccgcggt gagcgacagc gtcgcatcct ggcggccggc gctgccgtcg aaccgcgcgt
     1321 cgaggtcgat ggccccgctc agcacctgcc cggtccaggg ctcgagggcg gcgagatcgc
     1381 cgacccggcc ggcgagctgg ccggtggcga gcggcccgtc gagggcgaca gtcgccgccc
     1441 ccgtgagctc cgtgaaggga gcggtgaggc tgagattggc gagatcgagg ctctggccgt
     1501 cgagcgcgaa atcgctcttc agcgccagcg agtccgtgcc ctgctcgatc gccagggtga
     1561 gctcgccggc cggctccgcc agggggccgg aggtccgcgc gtcgagggcg acgcggtcga
     1621 actgccgctc ggcgatggcg aggcccacca ccgtgacgtc ggccgccacc tcgggcgccg
     1681 gcagggtgcc gccgagcgtc acgaagctcg tcagctcgcc ggcgatcggc tggccgacga
     1741 ggccctcgag ggtagccagc gccagaggcg agagatcgac ccggccgctc agcgcctggg
     1801 cctcgtcgag gccgaggtcg agattgccgg tgaggccgag ggcggccgtg gtcagctcca
     1861 ggctctcgac cgagacgctg ctgccgggca ccacgacgac atgcgaggtg agccgcggat
     1921 cggcgccgac cagcgcgtcg gcgttctgcg gcaggccggc gatgccggtg cccgcgccca
     1981 ccagatcgag cgtgatccgc ttcagctcat caaccatctc gatatcggcg cgcaggttga
     2041 aagcccccgc cacggcgagg tcgggcggtg ccgcggggcc gagggcggcg accagcgcct
     2101 cgagcgacgg cacgttcgcc tcgagccgcg ccgtcccacc gagcccgggc atcatcaggc
     2161 tggccgtgcc gtcgacggcg acatgctgtc cgtggagcgc cagccgctcg accacggcct
     2221 cgccctccat cggcacggtc agggcgagat cgagcgtcag ccggtcctcg ggtcggagcg
     2281 gctcgccatc ctgcgtgatg ccggcgatct cgccactgcc ttcgacccga gcaccggcga
     2341 agggctggtc gagcggcccg agcaggtcgg ccgtgatctc gaggccgagc gtctcgacgc
     2401 cgaaggcatc ggcggtgatg ccgccgccat cgaggtcgag gcgaaagcgc ggctcgctga
     2461 acgtgccgag ggcgtcgagc acgagctcga cccggcccgc caagggcgtt ccggcaagcc
     2521 cgctggcgcg gccgagatcg tccgccgcca cgcggatcga gccgtcgagc cggttgtcgt
     2581 cgagcagcgc ctcgagccgg ccttcggcgg tcgcgatgcc gctctccagc ctgagccgat
     2641 cgaggacgaa ctggccgggt gcccgctgcc cgccgaccac ggccagggtc agccggtcgc
     2701 cgagcacggc cgcgatatcc gcgggcatcg caccctcgac cggcaccacc tccgcctgga
     2761 ggtcgatcga cgggttctcg ccataggcga gcgccagccc ggcatcggcg gcgaccaggc
     2821 cctggacggc gagggcgaga tcggcgcgcc agttgtcgag cgggccctcg cccttcaact
     2881 gcagggtcag atcgcccgcc tgcccgatcc cggtgagcga ggccatgagc ccgcccgtct
     2941 cgctgccctc gacgtcgagg ccgatccggc gctgggccag atcgagcgtg gccgcgaggc
     3001 cgagcgaggc ggtggcctgg tcgatgcggg tgaggtccag cctggcctcg gcatggcgcc
     3061 cttccggccc ggtcgtggca ttgccctcga ggttgaagac cgcgtcctgg ccgatcaacg
     3121 cctggccgag ctcgatccgg tccacatgga tgcgctccgc cgtgacccgc ggcaggctct
     3181 ccggcagctc cggcgccgcc ggcaggctga agggctcgtc cgtctcgggc gccggcggcg
     3241 gctccggtgg gggcggcagg ttgggtgccc gctgcaccgc gacgcgggcc gccccggcct
     3301 cctcgatgac gacctcgccg cgcagcagcg gcgtcggcgc cagcttgacc cgcgcattgt
     3361 cgacctcgag ccagcggccc tcgtcatcgg cgagcgagaa gcggccgatg cggatgtcga
     3421 acggcaggaa gccgctcaga tcctcgacct ccgccgtctg ctgctcgccc tccaggctgc
     3481 tctcgatgag gccgctgatc tggtccttgg cgacacgggt ctgggcgagc gccagggccg
     3541 cgccgaagag cacgacggtg ccgaggacga tgccgagaag cgaaagcagg atcttgcgga
     3601 gcatggtctt gatctggtgc gggtgccgcc ggattgccaa ccttatggca tgccgcaaga
     3661 cccgccgcca tgcctgcccg ccgccatgcc cgcacggggc cggcccgggc tcggccgacg
     3721 ctcagaacgc ctggccgatc gagatgtaga gctgccaggc atcgtcgatg cccttgcgtc
     3781 ggtcgacggg cacaccgaca tcggcgcgaa tcggccccac cggggagatg tagcgcaccc
     3841 ccgcgccggt gccgaactgc caggcctcgt tgagctcggg cgtctgcggc tcgaaggcgc
     3901 tgccgccgtc gaggaagacg acaccctcga gattgccgac cacccgatag cgcacctcgg
     3961 cattggccgc gagcagcgag cggccgccgg tcggctcgcc gtcgtcgagc gggccggcgc
     4021 gctggaacgg cacgccacgc accgtgccgc cgccgccggc atagaagcgc tcgtcggcgg
     4081 ggatctcatt gatgctggcg ccgacgatgg acccggcgat gccgcggaac gcgaggacca
     4141 gccgcggatt cttggcgagg cggaaatagc gcgtgccggt gagctggctc ttgaggaact
     4201 gcgtcccggg attgagggta tcccagtagg gcgcggtttc cagaacgatg cgatagccgg
     4261 tcgacggatc gagcaggctg tcgctgcgat cgtagcgcag ccccgccggg atcgagagca
     4321 gggcgaagcg ctcctcctcg ttgtcctggt cctcgacatc ggcgagccgg taggcgacgc
     4381 cgagcgtgcc gctcagccgc ttgttgaaat gccgctcgac accgagcgag gcctggatcg
     4441 aggtgctctc gaaggcatcg gtgtcctcgt ggctcagcga gccgccggcc agcaggtcga
     4501 gatcgggcat gaagatgtcc ggcttgcgca gctggccttg cagccgctgc accgactggc
     4561 cgagctcggc ctcgacccgc agccgctcgg ccgcccccag gatgttgcga tgctcccaga
     4621 aggctcggac gttggggccc tcgtcgctga catacccgac gccggcgccg atggtccggg
     4681 gcttgcgctc ggtgacctcg aaggagaccg gccagttgcc gtcggcgtcc ggctccggcc
     4741 cctcgatcac ccgcacggcg gcaaagagat tggtgtcgat cagccgctgc tgggcgcgct
     4801 cgacatccgc cgggtcgtag cggcgccccg gcacgatctt ggtccgctgc tcgaggaagc
     4861 cgtcgtcgat gtcggtctgg ccgaaaacca ccggcgtcgc gaaatgaaag accggccccg
     4921 gctcgatcgc gagctcgaca tccatcgtcc gggtctccgg atcgatgatc gaccggcggt
     4981 cgcccaccct ggccatggca tgaccctgca ccctggcatc gcgcaggagg ctggattccg
     5041 ccgcgatcac gacatcggcc cgggcgggct ccccctcgcg cagcccgatc ttgccgggcg
     5101 acggcgcctc gtagccgtgc ggatcgccga tcacctcgat cttgcgatcg aggaaatggt
     5161 agagggggcc gggctcgacc tcgaacacga cgtgcgccgc caccgccccc agggcctggt
     5221 cgaccatgcc caccccggtc gccagcgtcg gcggtgccgg atcctcgtcg atgcggaagc
     5281 cgacggcgcc ctcgtaatag ccctcggagc gcagcgtcgc ctcgagccgg ggcacgtcgc
     5341 cgcgggcccg gttgcgcagg atcgcgaggc tgggcggcgg ccggccggtt tcctgggccg
     5401 ccgtcgacgc ggcgcggagc tggccgacga gcgtgctgtc ggcgggaccc tcgaagcgga
     5461 cgtcataggt gatgcggggc ccgctctggc tctcgtcgtc gcccagcccg cccccgttgc
     5521 cggcgctgca ggcggcgagc gccggcagca ggacaacggc caggagccgg cagaagcgcc
     5581 ggaggccggc gccggccgaa cggccccggg ccgcgccctg tgcccggcgg ccgatcggca
     5641 tcgccatcag gtgccgccgc gtcgtcgctg gccggtgtcc gcggcggcga tgctcaggga
     5701 cgggcgggct gcggccgtcg cctccgccgc cgccggaccg gcagcctcgt cgtccgccgg
     5761 cggctcggct gccgcctcat cggccctgag cgcgtggtgc gcccgctcga tgaacatggg
     5821 cggtgccctg ccgtccttgc cgacgccgaa atagacggtc tggtgcggga acgggatctc
     5881 gatgccgagc tcgtcgaagc gcagcttgat gcgcctgagg aactcgcgcc tgatgcccca
     5941 ttgctcgccc ggccgggtct tcgagcgcat gcggatcacg accgcggaat cgccgagcgc
     6001 atcgacgccc gcaatgtcga gcggctccac gatgaggcga cgatagggcc attccttgcg
     6061 catccgatcg tcgatctcgc gcagcacccg gatcacctcg tcggtgttct cgcggtaggc
     6121 cacgccgaca tccatgaccg cgaaggagaa ctccttcgtc atgttggtga ccacgtcgat
     6181 cgagccatag gggatcgtgt gggcgttgcc gtcgtagctg cgcagcgtga tcgtccgcat
     6241 cgacatgttc tccaccaccc cggccttgcc ggcgatgtcg atgacgtcac cgacccggat
     6301 cgtgtcgccg agcaggatga agaggccggt gatcaggtcc ttgaccagcg cctgcgagcc
     6361 gaagccgatg gcgaggccga cgacgccggc accggcgagc agcggcgccg cctccacgcc
     6421 cagatggccg agcgccgtga acagccccgc catgccgaag aagaagaagg cgacgttctt
     6481 gaagatgctg gccagcgtgc gcgagcggtt gctgtgccgc ggcttgccga actggtcctt
     6541 ggcattggcg aagcggttgg cgagatagcc ggtcagctcg aaggccgcga tcgtcgacgc
     6601 gacgatcagc atgatgctgc cgacgagggc gaggaagtcc ctgccggtcg gcgaggtcag
     6661 ccagtcgagg acgccggtgt tccagacgga gaggatggcg atcatcgccg ccaggctgac
     6721 ggcgccacgc agcagcaggc gcgccggccc ggcatagcga tcggcccgct cctggacgcc
     6781 cggcagggca tcctcgctgt caccggcggc cggcgcgcca tccgggagca ggcgcttgat
     6841 gcccagcgtc gccagccgtg ccacgaccag gatggcaaag gtcgaaaccg tcgcccggct
     6901 gagaaagatg atgccgttcg gcaccttgag ggcccagacc aggtagtgca gcagcacgag
     6961 gccgatggcg aaataggacc aggtgcgcgc aaggaagccg cccgggatga agcggaccag
     7021 cgggctctgg ctgttctcgt tccaggtgcg gatgccctcg gcgacgggct cgcgcacccg
     7081 gacgatgagc acgatcgcga tcccggcgac cgccagatac aggagatgga ccagaaaggt
     7141 gaagagcggc tccggcagcc ccaggaccag gagcgcatag agcacgccga agccgatcag
     7201 cccgagccgg ccgatgccca ggagaccccg ccggcagatg gccgcggtgc cgtcgccgac
     7261 ggatgcgagg tgcaggccga tgccggtctc gccgaacagc agccggacgc cggcgcccca
     7321 gagcatggcg acgaagagca ggccgccgag gctgagctcc gccccggcca ccctttgaat
     7381 gtccacgtca tagatcgcgg ccaccgaatt gctgatccag agcatgccga gggtcagccc
     7441 ggcgaacgtg acggcggcca cgatctcgat cgcgaagcgg ccgagcagcg tcgccttagg
     7501 gcgcgcggga tccggctcgg gtcgcagccg cagcaccatc gaccgggcgg cgaggcccac
     7561 cgcgaggatg agcacgacgg cgcccaggac gccgacccag acggtgcgct gctccgggtc
     7621 ggtgacctga tcctcgaccc actcgtacag cgccggcagc tgctcggtgg attcgatggc
     7681 gtcggtcagg atctcggcga ggttggcgct gcggcggcga agctcggtcg tcaggtcctc
     7741 gaccaccgtg accgccaccg tgtcgctggg tggcttggcc gagggcggcg cctcgccctc
     7801 ccgggcggca cgcagccggt cgagcagggc agcccggcgc tccggatcct cgagggtgcg
     7861 gatcaccgcc tcgatgtcgt cgctcgccga cgtatcggcg gccgctggtg ctgccggcga
     7921 catcggcatc atctgggcat gcgccgggcc ggcgagcgcc cagagcgcca ggacgccgag
     7981 gagcgcagcc atccagggcc ggagacgtcg aaaggtcatg aaggaaccga aaggtgatcg
     8041 gatgaccgag cttccagacg acggcatgtc gcgggccctc gcccggagcg gctgtcaagc
     8101 tgtacaacca ggacgcgagc ctactccacg ccgccacttt cgcaagaccg atgcgagcgt
     8161 agagcctcag ccggttttcg caagaccgcc gcgcagcgac tgcagggcct gctcgaaccg
     8221 ccaggccgag ccgacgatgg catcgaggct gttgccggcc ggccgccagc cgagggtacg
     8281 gcagagccgc cgattgtcgg ccaccaggac cgccgggtcg cccggccggc gcgggccgct
     8341 ctggacggga atgtcgatgc cggtcacccg ccgcaccgcc tcgatcacct cgcggacgct
     8401 gaagccgtgg ccgtagccgc aattggcgat cagcggctcg ccacccccca cgaggtgatg
     8461 cagggccagc acgtgcgctg ccgccagatc gtcgacatgc acgtagtcgc gaacgcaggt
     8521 gccgtccggg gtcgggtagt ccgtcccgtt gacgtgcatc gccggccgca ggccgatggc
     8581 cgtctcgagc gccaccttca ccagatggct gttgttcggc gtcgcctcgc cgatccgcgc
     8641 tgcggcgtcg gcaccggcga cgttgaagta gcgcaggatc gcgtagccga gcccggccgc
     8701 cgccgcgatg tcctgcagcg cccgctcggc catcagcttg ctggcgccgt agggattgat
     8761 cggtcggagc ggctggtcct cgtcgatcag ctcgacgtcg ggctggccgt agaccgcggc
     8821 ggtcgacgag aagacgacgt ggcggatgcc gtactgggcg caatgatcga agaagcgaaa
     8881 ggcgttgctg gcgttgttga aatagtactt ggcggggtgc cgcaccgatt cgccgaccca
     8941 gatgtgcccg gcgcagtgga tcgcggcgtc gaacgacgcg gccgacagca cgcgctcgag
     9001 gcgttgccgg tcgccgaggt cgccgatctc gagctcggcc cccggcaccg cccattcgtg
     9061 cccggaatag agattgtcga agacggtgac gcgatgcccg gcggcgatca ggtcacggac
     9121 gatgtggctg ccgacatagc cggccccgcc ggtgacgaga acacgcaatc cggtgggagc
     9181 ctcgggcatg gcagatcctt tgcgacacgt cgtgggacac actccccggg cggctcttgg
     9241 cagcgcgccg gcgaggcgcg gaccatatcc gctcagcagt cagatacgcg tgaacaaccg
     9301 gcggcggaag catatcttgc tacgctttgg aagagatccg ggacaccgcc aggccgcgca
     9361 gacgacgaag gtcagcgggg ttgcccggtc gagagaaacc ggtgatcggt gccttcgagg
     9421 accacgcagg tcaggttgcc ggtgtagcag gcgccggtgt cgatgccgat ccggttgcgg
     9481 cgaatggccg gtgcgtcctc gaccgtgtgg ccgtgcacga tgatcttgcc ggcgtcgccg
     9541 acccagctca ggaacggctc gcgaatccag atcaggtcct cctccacctg gtcggcgagc
     9601 gggacgccgg ggcggatgcc ggcatggcag aacagatagt cgccgaacgg cagggcgagc
     9661 tcgaggtcga cgagcagccg ccggtgggtg gccggcacct ccgtctgcag gcggtcgcga
     9721 gcttccgaca gacgctcctc gggcgagcgg tcgagcgcca gcggcacgcc gtagctgaga
     9781 agggtcgcgt caccgccgaa gcggagccag ctctcgccga ccggctcgcc ggcgagaaag
     9841 tcacggagcc aggcgtcgtg attgccgagc agcagcaccc ggtcgaaatc gtcgggtggc
     9901 tcgctcgcca gatggtcgag caccttgcgg ctgtcgaacc cgcgatcgac atagtcgccg
     9961 agatagacga gcaggttgcg gcaccgctcg gcgcgggacg cggcatcggc cacgatctgc
    10021 gcctccagcg cccgcagcag atcgatcctg ccgtgcacgt cgccgaccgc gtagacgcgg
    10081 gcaccctgcg gcacccgggg ttggcgacca ccgacccggg gtgtctcgtc gatcgccggg
    10141 cgcgggcgct ggaacagtcg cttcagcagg gttttctcaa ctcacaatag aattttgccg
    10201 cgacgagccc gacggcgccc ggacaactcg tggcaaacgc gttacagcat caatggtttg
    10261 cgggcaattc cgcacattgc gcagaaaatg ccctaccctc cggttgtcac gcctcgccaa
    10321 accggataga tgcaccgatg gttgatccgg ttgtcgccgg tggcgtggcc gctcgcgtcg
    10381 tcgttcgtcc gagcaagttc tagcgccgtt catgaccgca tgcacgcggc atgtgcgaat
    10441 cggatggagc gggcgttcga cggttcttga ttccgggtgc ccggcagacg atgaccgacg
    10501 ggcaagcggg tgcagggccg ttgctcggca gcagccgtcc gaggggcacc tcctgaagcg
    10561 gagtagagtg atgacgacga ccaggctgat cggcgctctc gtggccgggg cggctgtgct
    10621 ggcaccgctc gcgacggcgg cccaggacat tcgcaacgtg ccggtgcaga accggccgcg
    10681 ccccgactac gacccgctgg gcattcgggc acgggccttc ctgatctatc cggaaatcga
    10741 aaccggcgtg gcctatgacg acaacatctt ctcgacgagc gacaacgagg atgacgacgt
    10801 gatcttcacg gtgcgtcccg cgatcaccgc caggtcgcaa tggtcccgtc accagctcaa
    10861 tctctccgcc ttcggcgatt tcggcttcta cgccgaccac aacgacagca actaccagga
    10921 cttcggcgtc ggtgccggcg gtcgcatcga tgcgctgcgc aacagccgga cgaacatcca
    10981 gaccaacttc gcccgcctgc acgaggactc cgaggaggcc gacgcccagg gcaacgagga
    11041 cgtgacccag tactaccgga tgggcatcaa cctcagccac cgccacgagt tcacccgcgt
    11101 ctacgtcgag ccgatcgccc ggttcctgcg cctcgactac gacaatgccg gcgatgtcag
    11161 caacaccgac cgcgaccgca acgagtacgc gctcgggctg cggacgggct acgcgctctc
    11221 gccacgcatc gcccccttct tcgagctgac cggcagtgcc cgggaatatg acgagacccc
    11281 ggggctcgat cggaactcga ccggcttcga cgcgctgatc ggcaccacca tcgatttcac
    11341 cggcgtgatc ttcggcgagg tctccgccgg ctacacgcgg cgcgagtacg acgacaacga
    11401 cctcgagaat gtcgacgggt tggccggcaa tgccgcggtc acctggaacg tgacccccct
    11461 gacctcggtg atcttcgacg gccaggcggg catcgacgag acgacgatca cctacgaggg
    11521 cgacacggcg accgccaaca actacgccgg cctcgcgatc aacgtcttcc acgagctcag
    11581 gcgcaacatc atcgtcaatg tcaatggcga gtatgtgcgc gacgactacg agggcacggg
    11641 gcgaaccgac aacacctacc gagccggtgc cggcgtcacc tatctgatca accgccgctt
    11701 ctcgctcgac gcgacctacg acttcagcca tcgccagtcg gacgacgggg atgcggagtt
    11761 cgaccgcaat atcttccgca tcggcctctc cgccaagcta tgagcggcag tcgcatcgcg
    11821 gcacctgcct gcgatgcccg gcgccgccgt cctctgcttg ctcgaccatg gctttattct
    11881 tgagtaacgt tcgccgtgta gtcaggtacg gatgggcgtc gtcgccggct gcttgcggcg
    11941 aatcggacgc atggacgtgg tgcgtgagag cggtggagag agaatgatga cgacgacttg
    12001 ccgcaagctc ctggcggggt tggtgctcgg gctggggctg ctgctcagcg cctgtggcag
    12061 cccatcgccc ggcacgctcg gccccgctgc cggcgccggc acgggggcgg acggcacgcc
    12121 ggcggcgcgc ctcagcgctc ccgcctacac actcgacagc ggcgaccgga tccgcatcgt
    12181 ggtcttccgc cacgaggatc tctccggcga gttcgagatc gatggcggcg gcacgttcgc
    12241 catgccgctg atcggcgaga tcaacgccgc cggcctcacc gcgcgccagc tcgaggaccg
    12301 catcgccacc aggctcaagg acggctacct cgtcgacccg caggtcagca tcgaggtgct
    12361 caactatcga cccttcttca tcctcggcga ggtcaacgcg cccggcagct atccctacgt
    12421 ctcgggcatg agcgtgatca acgccgtggc cctcgccggc ggcttcacct accgggccaa
    12481 ggaggatggg atcgagatcc agcgcggcgg ctcgggcgcc ccctccgtgg tggtgacggt
    12541 cgacacgccg atcctgccgg gcgacatcct ccgcgtcccc gaacgcttct tctgagccag
    12601 cggtgcagac cggcaccgcc gccgccccgg tgccggcggc ggttgtctgt cgtgcggcag
    12661 ggccgcaagc gctgttgacg gagggcatcg atggctgagg cgcggcaggg caagccgggc
    12721 tggctggact ggcggagctg gctcgcctcc ctcgcgatct atcgcgaccc gagggtcgtc
    12781 ggcgtgctgt tcctgggatt ctcgagcggc ctgccgatca tgctcatcgc cgcgacgctc
    12841 agcacctggc tgcgcgagga ggaggtgacc cgggccgcca tcggcctctt cggcttcgcc
    12901 ttcgccccct acaccttcaa gttcgcctgg gcgccgctca tggaccggct gcctttgccg
    12961 ccgttcacga gcctgctcgg ccggcgccgc ggctggatga tcgcggcgca gatcggcctc
    13021 gtcctctcga tcttcgcgct cggccggacg agccccgccg aggacctctg gtggaccgcc
    13081 ttcttcgcgg tgctcgtcgc cttcttctcg gccagccagg acatcgtcat cgacgcctat
    13141 cgcgtcgaga tcctgccgaa ggagaagctc ggcgccggcg ccggggtcgt cgtgctcggc
    13201 tatcgcgtcg ccatgtgggt ggcgaccgcc ggtgccctgc tgatcgccga ggccttcggc
    13261 tggtccgctg cctacacggt gatggcgctg ctcgtcctcg tcggcatggg cacggtgctg
    13321 tccatcggcg agccggcgca gtcctcgacc gccctcatcg accaggaggt gctcgaggag
    13381 gaccgcctgc tccgccagac cacaccagcc aggctcgctc ccttcaaggg cctcggcggg
    13441 ctcctcctgg cggccgtgat cggtctcggg gtgtggcagc cgctctcgac cctcttcggc
    13501 atcgtgacgg caccaccgga atcctggtcg atctcgggcg ccatcgtgct cgccggcaac
    13561 ctcgccgtca cggcgctcgg catgcggctc gcctggctgc tctaccgccg ggcacccgat
    13621 gccctcgccc ggaccaagaa cttcgccctg ctcgccatcg cctgggcgac cgtcgagaca
    13681 ctctggtatg caatcgggcc agaggcgccc ttcttcgcca cgctgcacgg ctggctggtg
    13741 ctcggcctcc tctttctcgt cagcaactgg gtcgtcggcc tcttcggtgc ggcaccgttc
    13801 acctacgagg ccttcatggc cccggtctgg ctgaccgtcg tcggcttctg gctcgagctc
    13861 gtcgccctgc tcacgctcta cggcttcacc tatttcagcc gccgggcgca ggccaatttc
    13921 gggatggtgc cgatcgccga ggagagcagg gtccatctct ggatgcggcg ggcggtgatc
    13981 gagccgttct acgacttcgt caggcgcaac gggctccggg tcgccgtcgt catcctcgcc
    14041 ttcatctcgg tctacaaggc agccgacgtc gtcctcaccc tgatggcgaa ccccttctac
    14101 atcgatgtcg gcttcgacaa ggcggagatc gcctgggtct cgggcacgtt cggcctctgg
    14161 atgaccctga ttggcggcat cctcggcggc gtgctggtct tccgcctcgg cctgatgctc
    14221 tccttggccc tctccgtggc gctgatggcg ggctcgaacc tgatgttcct gctcctgtcc
    14281 tgggtcgggc cgaacctcgc cctcttccac gccgtcatcc tggtcgagaa cgtcaccgcc
    14341 ggcctcggca cgaccgtctt cgtcgcctac ctctcctccc tgtgcaacat ccactacacg
    14401 gcggtgcagt acgccttgct cacctccttc atgcagctcc tcggcaagtt cgtcatcgtg
    14461 ccgtcgtccg gcttcttcgc ggacgccgtg ggctggtcgg tcttcttcct gacctccacc
    14521 ctcttcgccc tgcccgccct cttcctgctc tggctgctgt ggcgctgggg cctggccgtc
    14581 gagacaccgg cggcagcacg cgcctaggag cctggccatc tctcggccag gctgctaggc
    14641 cggcacctcc tgcatcgagc gggcatccgc ctcgcggcgt tccagcttgc cgaagatctc
    14701 cttcagccgg ctcaggctgt agggcttgac gatgaagccg aaggcgccct ggcgcagcgc
    14761 cttgacgacg acgtcctggg tgctgtgatg ggtgaccatg acgatcggca gcgccgggtc
    14821 ggc
//
LOCUS       DAOTFI010000117        14819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4857_length_14819_cov_6.557843, whole genome shotgun sequence.
ACCESSION   DAOTFI010000117 DAOTFI010000000
VERSION     DAOTFI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14819
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4857_length_14819_cov_6.557843"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="P5558_13015"
     CDS             <1..865
                     /locus_tag="P5558_13015"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY25280.1"
                     /translation="YDEAEPLYLRALALDEAKSGTDDPNLAASLNNLALLHRAQGRND
                     DAERLYKRSLRLLEDAYGHSHPQVAMSLNNLAMLYVATDHPERARPLLERAEGVARDT
                     LGPTHPSTLRIAQNLRALDGGTPAGTDPLGFELRPSPRPGSTPLPAPAPAAVAAPEVA
                     ASPPEAAPTAASGDVVPVPAPPIPEPAPEVAVAAVPAVKPPLPRGFAIHLASVRSPAA
                     ANEEWTRLATELDLDPRIRQVLPQRVEVPGKGVFYRVEGGPFATRAEAAAACAPLAAA
                     GQYCAVIESGD"
     gene            870..1637
                     /locus_tag="P5558_13020"
     CDS             870..1637
                     /locus_tag="P5558_13020"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY25281.1"
                     /translation="MDWPAAIEAQLLDALAGSGAEPVEAGGFRVHLWHQPDPFYRNVA
                     VPVARPADWPPAIRLMDGVFRAAGRPARLEFIEERWPDLALALGDAGFVRRQRMPVMI
                     LDRPGTGAPASEVELLGPGTAPDLVAATLDALNAAFGQSMTESTRAAETRQMVEDLGS
                     GRTRVAVVLMDGDPVAGACLIGNGRAAELAGVWTAPGYRRCGLGTAACRALLDRFLTD
                     GGRFAWLGAHGDAARALYARLGFRPVGHQLDYSRPDA"
     gene            1696..2709
                     /locus_tag="P5558_13025"
     CDS             1696..2709
                     /locus_tag="P5558_13025"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25282.1"
                     /translation="MPLNRRTTSRVLAGLAALLTCLPAPAPAAEKVVFGTNWRAQAEH
                     GGFYQALATGLYAERGLDVEIREGGPQVNHRQLIAAGRLDFYMGGNMFGQFDFLREGV
                     PVVTVAAIFQKEPQALLAHPGVGIDDLDDLAGRTLLIAPFGQLTFWPWLKGTYGLDDA
                     QIRPYTFNPAPFIANPDAVQQGYVTAEPFAIREMGGFDPVVLLLADAGYDSYSTLIQT
                     RQELVEQRPEIVQAFVDASILGWMDYLYGDPGPGNALILAANPDMTPEQIAYSIDAMK
                     AYGIVDSGDALELGVGAMTDARWESFFLQAVDWGIVEPDLPWRAGYTLEFVNRGVGNE
                     LRP"
     gene            2802..2969
                     /gene="rpmG"
                     /locus_tag="P5558_13030"
     CDS             2802..2969
                     /gene="rpmG"
                     /locus_tag="P5558_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRY25283.1"
                     /translation="MAKPSTVLIKLVSSAGTGFFYTTRKNTRTTTEKLVLKKYDPRAR
                     KHVEFKESKIK"
     gene            3189..4418
                     /locus_tag="P5558_13035"
     CDS             3189..4418
                     /locus_tag="P5558_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25284.1"
                     /translation="MAQSAVSRLGNRCIVGLAAFLACTALTRAALAIDDLLSEPTPLI
                     LKDGRIAQVSVHTIPFATGSGELDAEAAAELDALLVPLATDCFLTAQAIGHVEPGLNR
                     DGDTLSAHRLARARADFVQAALAGHGMQLAAVASVWDWQFLVKESRVTLWVFRLNVGD
                     DCEGTPLPSDLPVAAAAAPVVDAVSPPAAAAPAPVKPPAKSPPPAEKSSTVATNGVAT
                     TDPRELSTAEAPPPATAAAAATPAPAIEPPAPAIEPPAVMAEAKEPAPVEAPAPVAPA
                     AEPEPIEVAAPLPATADSPPAPVAELPGPAIVFAVNSSFFSGSASRDLRAFVDQLPLD
                     AEVEIELAAAVGTGDVRDADPVEAKRYNAWMAERRLQRVAEWLTSNAGPRQLKLNEHY
                     IENDPSREVRLRARVLP"
     gene            4511..5419
                     /locus_tag="P5558_13040"
     CDS             4511..5419
                     /locus_tag="P5558_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY25285.1"
                     /translation="MTLAIGIDLGGTKIEIVALDGEGREVVRRRQPSPRGDYQATLDA
                     MAGMIRTVEAELGARGTVGVGHPGAISPATGLIKNANSTWLNDRPLDRDFQALLGRDV
                     AFANDANCLALSEATDGAGAGADIVFTVILGTGVGGGIVVGGKVLRGGNAIAGEWGHN
                     PLPWPRDDERPGPPCYCGLHGCIETWLSGPGMARDHEAVTGQALSGPGIVAAAAAGES
                     AARATLDRHADRLARGLAHVINILDPEVIVLGGGVSNIGGLYAEVPPRLPAYVFSDRV
                     DTRLLPPLHGDSSGVRGAAWLGRDLT"
     gene            complement(5435..6235)
                     /locus_tag="P5558_13045"
     CDS             complement(5435..6235)
                     /locus_tag="P5558_13045"
                     /inference="COORDINATES: protein motif:HMM:NF020478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1849 family protein"
                     /protein_id="HRY25286.1"
                     /translation="MSTVRRLACLVVGALAALGSAAADARVDLLSHRAVYRLSLADSD
                     RSIGVTSVDGGLVLEWRASCEGSISNQRIGFVASLGDGPGFVYDVRFSSWESPDNTRL
                     RFNVRSFDDGHLFEEYRGEAKLDGKGGAGHASFVAPADEEIELPRGTLFPTEHMRRLV
                     ESAERGDVVVSHDLFDGSGLEGLSRVTAVIGRAQAAERPSPDDAERSWPVSLAYFTTA
                     GVDDLPSFEIMFDLTERGVLHDLVLDYGDFALKAEMEQLETFPAPDCR"
     gene            6676..9387
                     /gene="acnA"
                     /locus_tag="P5558_13050"
     CDS             6676..9387
                     /gene="acnA"
                     /locus_tag="P5558_13050"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRY25287.1"
                     /translation="MRVCGQDTLKVRRSLDVDGARYDYFSLPEAEKRLGELGRLPFSL
                     KVLVENLLRFEDGSTVTVAHLEAAAAWLKDRRSDQEIAYRPARVLMQDFTGVPAVVDL
                     AAMREAMVELGGDPQRINPLSPCDLVIDHSVQVDSFGGPGSFERNVELEYQRNGERYA
                     FLRWGQGAFDNFRVVPPGTGICHQVNLEHLAQVVWTGKDGNAEIAYPDTLVGTDSHTT
                     MVNGLAVLGWGVGGIEAEAAMLGQPVSMLLPEVVGFKLTGRLPEGTTATDLVLTVTEM
                     LRAKGVVGKFVEFFGPGVGQLPLADRATIANMAPEYGATCGFFPIDDVTLEYLRLTGR
                     DDRRIALVEAYAKAQGMWRSDDLPDPVFTAVLELDLARVEPSLAGPKRPQDRVALGAV
                     KDSFAKALPGLLGKAPADKVQPIAGMDAELRHGDVVIAAITSCTNTSNPSVMLQAGLV
                     ARKARALGLQPKPWVKTSLAPGSKVVTDYLAAAGLQEDFDAMGFDLVGYGCTTCIGNS
                     GPLPDAVAAAVDEGGIVVGAVLSGNRNFEGRVHPQVKANYLASPPLVVAYAFAGTLTR
                     DLTTEPLGKGKDGQLVYLKDIWPDQAEVAEVVRTAVTAAMFKKSYSDVFKGDERWAAI
                     GGGDSGVTYDWDDGSTYVRLPPYFEGMEAVPPASVPDIEEARVLAILGDSITTDHISP
                     AGSIAQASPAAEYLQSFQIQRKDFNSYGSRRGNHEVMMRGTFANIRLRNELADGKEGG
                     WTKLMPEGEPMSIYDAAMKYKERGTPLVIFAGKEYGTGSSRDWAAKGTNLLGVKAVIA
                     ESFERIHRSNLVGMGVLPLTFENGESRSSLKLDGSEVVTLEGLAAGLKPRMKVACTIR
                     RTDGSETKTSLLCRLDTGDEVDYYRHGGILHYVLRSLLGATPAAGRA"
     gene            complement(9451..9702)
                     /locus_tag="P5558_13055"
     CDS             complement(9451..9702)
                     /locus_tag="P5558_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25288.1"
                     /translation="MTTRNRASRLVAGVALIGLLSGCGVLDVDDKTGQRAATGAAVGA
                     AAGAAVGVLTGGLLASTVAGAVVGGAGGFIFDQADRAGN"
     gene            complement(9729..10082)
                     /locus_tag="P5558_13060"
     CDS             complement(9729..10082)
                     /locus_tag="P5558_13060"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HRY25289.1"
                     /translation="MRQGLTVLAGAALLAAAASAEASPFERQTTGELQAICASPAELD
                     QHFCIGFILGAGQLYAEMLRIEAIEPLACPEPEPTLTVLRDTFVGWAGAHPEEADTRA
                     IDGMMQAAAETWPCN"
     gene            10208..10912
                     /locus_tag="P5558_13065"
     CDS             10208..10912
                     /locus_tag="P5558_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY25290.1"
                     /translation="MATDRVALITAGGSGMGADAARRLAADGFRLAILSSSGKGEALA
                     AELGGVGVTGSNRSIDDHRRLVDLAMQRWGRIDVLVNSAGHGPKGPILAISDEDWHLG
                     MEVYLLNVIRPARLVTPIMVAQGGGAIINISTFATFEPDPLFPTSGVFRAGLASFAKL
                     FANRYAADNVRMNNILPGFIDSLPETEDRRARIPMGRYGRAEEVSGLIAYLASDAAAY
                     VTGQNLRIDGGLTASV"
     gene            10924..11439
                     /locus_tag="P5558_13070"
     CDS             10924..11439
                     /locus_tag="P5558_13070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY25291.1"
                     /translation="MSKPLRIARAQVREAGAIARLIEPAFARFVAPDLGEVGRVAFRL
                     YVTEKALRRRLMDGAVGFRASLGEALVGYAELRGRDGRIDGLDHLTLLFVAVDLQGRG
                     IARRLLDVITGHLAAARPPVGDLTVNASAYAVPIYHRLGFRATGEAGEFQGVVATPMR
                     LELDPGGTQAV"
     gene            11482..11724
                     /locus_tag="P5558_13075"
     CDS             11482..11724
                     /locus_tag="P5558_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25292.1"
                     /translation="MAGMGEDWRLLIFLGKNCALGIAAGWLLLAALIRANVGGLGDLL
                     FGNGLELTALVLVAAGFAVTFGGLAMGTAVFLLPRD"
     gene            11796..12347
                     /locus_tag="P5558_13080"
     CDS             11796..12347
                     /locus_tag="P5558_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25293.1"
                     /translation="MSGAGTVREAVAVMEDVAALELAVEALEEAGFADSDISLLAGHG
                     TVEGKLGRMYRRVEEAEDDPAAPRTAFVSTRTVARREDMVVGSMTTLPTLIAAGTVVA
                     SAGAVAAAIAGTAITGALIGTVLARWMDKRHADWLEEQLERGGILLWVRTPDEAAERK
                     ALRILGEHAAHDIHIHEIPKPAD"
     gene            complement(12360..13127)
                     /locus_tag="P5558_13085"
     CDS             complement(12360..13127)
                     /locus_tag="P5558_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HRY25294.1"
                     /translation="MIDLVGRRAEIVALVQRQGFVTIEALAQQFGVTPQTIRRDINEL
                     AAEGVLQRYHGGAAVPSSVHNIAYSSRQVMHLEAKRRIAREVARHIPDDASLCINIGT
                     TNEEIAKALLDHRGLSVITNNLHVAELMAGNEDFQVVVAGGLVRARDRGIVGEATIDS
                     IAQFRVDIAVIGISAIDLDGTLLDFDYREVRVAQTIIGHARKVYLAADHSKIGRPAMA
                     RLGDMSQIDAFFTDRAPPPELAAVLESCGTRLVVAGD"
     gene            complement(13124..14632)
                     /gene="glpK"
                     /locus_tag="P5558_13090"
     CDS             complement(13124..14632)
                     /gene="glpK"
                     /locus_tag="P5558_13090"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRY25295.1"
                     /translation="MAADLILAIDQGTTSTRSILFDATGRPIATAQRELQQLYPADGW
                     VEHDPEHIWADTLSTCEWVLERAGVPIARVAAIGITNQRETAIVWERASGRAIANAIV
                     WQDRRTAPLCRELVADGLTETVAARTGLVIDAYFSATKVAWLLDNVPGARARAERGEL
                     AFGTVDCFLLWRLTGGEVHATDAANAARTMLFDIGRQDWDEELLERLGIPRSMLPEVK
                     DNSGLFGTTDPAVLGAAIPVTGMAGDQQAATFGQACFEPGMLKSTYGTGCFALLNTGG
                     RMVPSRNRLLTTVAYRLDGKTTYAIEGSIFIAGAAVQWVRDGLRAIGNAAESEALARD
                     LPDTGGVYLVPAFTGLGAPYWDPDARAAILGLTRDSGLRHIVRAALESVCYQTADLMA
                     AMKDDIEAADAGVLPETLRVDGGMVANDWVCQYLADILDCPVERPAVVETTALGAAFL
                     AGLAIGLYPSQAFIAESWRADGRFTPQLDPSTRRRRLDGWAEAVGRVRTRGR"
BASE COUNT         2140 a         5455 c         5059 g         2165 t
ORIGIN      
        1 ctacgacgag gccgagcccc tctatctgcg cgccctggcg ctggacgagg cgaagagcgg
       61 cacggacgat ccgaacctcg cggcgagcct caacaacctc gccttgctcc atcgcgcgca
      121 ggggcgcaac gacgacgccg agcgcctcta caagcggtcg ctgcgcctgc tcgaggacgc
      181 ctacggtcac agccatccgc aggtcgccat gagcctcaac aacctggcca tgctctacgt
      241 ggcgaccgac catccggagc gcgcccggcc gctgctcgag cgggccgagg gagtcgccag
      301 ggacacgctg gggccgaccc acccgtcgac cctgcgcatc gcgcagaatc tgagggcgct
      361 cgatgggggc acgccggccg gcacggaccc gctgggcttc gagctgcgcc cgtcgccgcg
      421 tccgggctcg accccgcttc cagctccggc cccggcagcg gtggcggcac ccgaggtcgc
      481 agcgagcccg cccgaggcgg caccgacagc agcgtccggc gacgtcgtgc ccgtgcccgc
      541 gccgccaata cccgagccgg cgcctgaggt cgcggtcgcg gccgttccgg cggtgaagcc
      601 gccgcttccc cggggtttcg ccatccatct cgcttcggtg cgcagcccgg ccgcggcgaa
      661 cgaggaatgg acccgcctcg ccaccgagct cgacctcgat cccaggatcc gccaggtcct
      721 gccgcaacgc gtcgaggtcc cgggcaaggg ggtcttctac cgcgtcgagg gcggcccgtt
      781 cgccacgcgg gccgaggccg ctgccgcctg tgcgccactc gccgctgccg gccagtattg
      841 cgccgtcatc gagtcgggtg attgagcggg tggactggcc cgcggcgatc gaggcccagc
      901 ttctcgacgc cctggccggc agcggtgccg agccggtcga ggcgggcggc tttcgtgtgc
      961 atctctggca ccagcccgac cccttctacc gcaatgtcgc cgtgccggtg gcccggcccg
     1021 cggactggcc gcccgcgatc cggctcatgg acggcgtctt tcgggcggcc ggccgccccg
     1081 cccgcctcga attcatcgag gaacgctggc ccgatctggc actggcgctc ggtgatgccg
     1141 gcttcgtccg ccgccagcgc atgcccgtca tgatcctgga tcgcccgggc accggtgccc
     1201 cggccagcga ggtggagctg ctcgggccgg gaaccgcgcc cgatctcgtc gccgccacgc
     1261 tcgacgccct caatgcggca ttcggccagt ccatgaccga atcgacgcgt gccgccgaga
     1321 cgcggcagat ggtcgaggac ctcggctccg gccgcacccg ggtcgccgtc gttctcatgg
     1381 acggcgaccc ggtggcgggc gcctgcctca tcggcaacgg gcgagcggcg gagctcgccg
     1441 gcgtctggac cgcgcccgga taccgccggt gcggcctcgg cacggctgcc tgccgggccc
     1501 tgctcgaccg cttcctcacc gacggcggcc gattcgcctg gctcggcgcc catggtgacg
     1561 ccgcccgggc cctctacgct cgtctcgggt ttcgcccggt cggccatcag ctcgactatt
     1621 cgcggccgga cgcttgaagc cgaccggccg ctgcgccacg ctcatcaagg acgccccatg
     1681 acacggacgg gatcgatgcc cctgaacagg cggacgacaa gccgggtgct ggccggtctg
     1741 gctgcgctgc tgacctgcct gccggcgccg gcacccgccg ccgagaaggt cgtcttcggc
     1801 accaactggc gggcccaggc ggagcatggc ggcttctacc aggcgctggc aaccggcctt
     1861 tacgccgagc gcgggctcga cgtggagatc cgtgaaggcg ggccgcaggt gaaccaccgc
     1921 cagctcatcg ccgccgggcg gctcgacttc tacatgggcg gcaacatgtt cgggcagttc
     1981 gatttcctgc gcgagggcgt gcccgtcgtg accgtcgccg cgatcttcca gaaggagccg
     2041 caggcgctgc tcgcccaccc cggcgtcggc atcgacgacc tcgacgacct cgccggccgc
     2101 acgctcctga tcgccccttt cggccagctc accttctggc cctggttgaa gggcacctac
     2161 ggcctggatg acgcgcagat ccggccctac accttcaacc cggcgccgtt catcgccaac
     2221 ccggatgccg tgcagcaggg ctatgtcacc gccgagccct tcgccatcag ggagatgggc
     2281 ggcttcgacc cggtggtcct gctcctcgcc gatgccggct acgacagcta ctcgaccctg
     2341 atccagaccc gccaggagct ggtggagcag cgcccggaaa tcgtccaggc cttcgtcgac
     2401 gcctcgatcc tcggctggat ggactatctc tatggcgacc ccgggccggg caacgcgctg
     2461 atcctcgcgg ccaatcccga catgacgccg gagcagatcg cctactcgat cgacgccatg
     2521 aaggcctatg gcatcgtcga ttccggcgat gcgctggagc tcggcgtggg tgcgatgacc
     2581 gacgcacgct gggagagctt ctttttgcag gcggtcgact ggggcatcgt cgagccggac
     2641 ctgccgtggc gcgccggcta tacgctggag ttcgtcaacc ggggcgtggg caacgagctg
     2701 cggccctgag tgccggcttc tgcaacgccg ccgatcttga caggacggcg cccatccccg
     2761 atctttcggg ctcccgcatt ccagttttcg aggatccagc gatggccaag ccgtccaccg
     2821 tgctgatcaa gctcgtgagc tcggccggta ccgggttctt ctacacgacc aggaagaaca
     2881 cccgcaccac caccgagaag ctcgtgctca agaagtacga cccgcgtgcc cgcaagcatg
     2941 tcgagttcaa ggaatccaag atcaagtagc gaccagggcc atgcgcggcc ttcgcttcgc
     3001 gcatggcggc gctgcagctc ggcacgagag cccgccgctc gcaggcgggt ttttgctcgc
     3061 tcaagtcgaa agtctctagg catttcgatc taccacaggt agtatccggc ccggttcgcc
     3121 attgtgctgg ccggtgcggt gcggcgtcgc cgccgcgtgg ccggtcgtga agcctggaga
     3181 catcgcccgt ggctcagagc gccgtttcgc gtttaggcaa tcgttgcatc gtcggcctcg
     3241 ccgccttcct cgcctgcacg gcgctgacca gggccgctct cgccatcgac gacctgctca
     3301 gcgagccgac gcccctgatc ctcaaggacg ggcgaatcgc ccaggtcagc gtccacacca
     3361 tccccttcgc caccggcagc ggcgagctgg atgccgaagc cgcggccgag ctcgatgcgc
     3421 tgctcgtgcc gctggcgacc gactgcttcc tgaccgccca ggcgatcggc catgtcgagc
     3481 ccgggctcaa ccgtgacggc gacacgctga gcgcccatcg gctggcccgt gcccgcgcgg
     3541 atttcgtcca ggcggcgctc gccggccatg gcatgcagct cgccgccgtg gcgagcgtct
     3601 gggattggca gttcctggtc aaggaatccc gcgtcacgct ctgggtcttc cggctcaatg
     3661 tcggtgacga ctgcgagggc acgcccctgc cgagcgacct gccggtcgcc gcggccgcgg
     3721 ccccggtggt cgacgcggtg tcgccgccgg cggctgcggc cccggccccc gtgaagccac
     3781 cggcgaagag cccgccgcca gccgaaaagt cgtcgaccgt ggccaccaac ggtgtggcga
     3841 ccaccgaccc gcgcgagctg agcaccgccg aagcgccgcc gccggcgacc gccgccgcgg
     3901 ctgctacccc ggccccggca atcgagccgc cggccccggc aatcgagccc ccggccgtca
     3961 tggccgaggc gaaggagccg gcacccgtcg aggcgccggc cccggtggcc cccgcagccg
     4021 agccggagcc gatcgaggtt gccgccccgc tgcccgccac agccgactcg ccgcccgcac
     4081 cggtcgccga gctgcccggc ccggccatcg tcttcgccgt caacagcagc ttcttctccg
     4141 gctcggccag ccgcgacctc agggccttcg tcgaccagtt gccgctcgat gccgaggtcg
     4201 agatcgagct cgccgccgcc gtcggcaccg gtgacgtgcg ggatgccgat ccggtcgagg
     4261 ccaagcgcta caatgcctgg atggccgagc gacgcctgca gcgcgtcgcc gaatggctca
     4321 ccagcaatgc cggcccgcgg cagttgaagc tgaacgagca ttatatcgag aacgacccgt
     4381 cccgcgaggt ccggctccgg gcccgcgtgc tgccgtagca ggcgagccgc cgcgactggt
     4441 cgcggcggtg ccggccccct atgctcgtgt cgccggtcgc ccggccggcg acactgatcg
     4501 gggggacggc atgacactcg ccatcggcat cgacctcggc ggcaccaaga tcgagatcgt
     4561 cgccctcgac ggcgagggca gggaggtggt ccggcgccgc cagccgtcgc cgcgcggcga
     4621 ctaccaggcg accctcgacg ccatggccgg catgatccgg acagttgagg cggagctcgg
     4681 cgcaaggggc acggtcgggg tcggccaccc gggcgccatc tcgccggcca ccggcctgat
     4741 caagaacgcg aactccacct ggctcaacga ccggccgctg gaccgcgact tccaggcgct
     4801 cctcggccgc gacgtcgcct tcgccaacga cgccaactgc ctcgccctct ccgaggcgac
     4861 cgacggcgcc ggcgcgggcg cggacatcgt cttcacggtc atcctcggca ccggggtcgg
     4921 cggcggcatc gtcgtcggcg gcaaggtcct gcgcggcggc aacgcgatcg ccggcgagtg
     4981 ggggcacaac ccgctgccct ggcccaggga cgacgagcgg cccggcccgc cctgctattg
     5041 cggcctgcat ggctgcatcg agacctggct ctccggcccg ggcatggccc gcgaccacga
     5101 ggcggtcacc ggccaggcgc tgagcgggcc cgggatcgtc gccgccgctg ccgctggcga
     5161 aagcgctgcg cgcgcgaccc tcgaccgcca cgccgaccgc ctcgcccgcg ggctggccca
     5221 cgtcatcaac attctcgatc ccgaggtcat cgtcctcggt ggcggtgtct cgaacatcgg
     5281 cgggctctac gccgaggtgc cgccgcgcct gccggcctat gttttctcgg accgtgtcga
     5341 cacgcggctg ctgccgcccc tgcacggcga cagctccggg gtgcgcggcg ccgcctggct
     5401 cggccgcgac ctcacctgac cgccgcgccg cgatctatcg gcagtcgggg gcggggaagg
     5461 tctcgagctg ctccatctcg gccttgagcg cgaaatcgcc gtagtcgagc acgagatcgt
     5521 gcagcacgcc ccgctcggtc aggtcgaaca tgatctcgaa gctcggcagg tcgtcgacgc
     5581 cggcggtggt gaagtaggcg aggctgaccg gccagctccg ctcggcatcg tccggcgacg
     5641 gccgctccgc cgcctgggcc cggccgatca ccgccgtcac ccgactcagg ccctcgagcc
     5701 cggagccgtc gaagaggtcg tggctgacca ccacgtcgcc gcgttccgcg ctctcgacca
     5761 gccggcgcat gtgctcggtc gggaagagcg tgccgcgcgg cagctcgatc tcctcgtccg
     5821 caggtgccac gaacgacgca tgaccggcgc cacccttgcc atccagcttt gcctcgccgc
     5881 gatattcctc gaagagatga ccatcgtcga acgagcgcac gttgaagcgc agccgcgtgt
     5941 tgtccggcga ttcccagctc gagaagcgca cgtcgtagac gaagcccggg ccgtcgccga
     6001 ggctggcgac gaagccgatc cgctggttgc tgatcgagcc ctcgcaggac gcccgccact
     6061 ccagcaccag cccgccatcc accgaggtga cgccgatcga ccgatcggag tcggcgaggc
     6121 tcagccgata gaccgcgcga tgcgacagca ggtccaccct ggcgtcggcg gcggcggagc
     6181 cgagcgcggc cagggcgccg acgaccaggc aggcgaggcg acggacggtg gacatcggcg
     6241 ttcccattct tgcttgctgg cggtgcccgg ccgcgggtcg gcacgggcac tgcctgtctg
     6301 tcttggtata tggcgttgct gcggcgaagt tccgagccct cagcttaacg gttcattagc
     6361 acaaccggcc gccccggtcg tcgggacggg cggtgatgcg cataccccgc ggcagcgaaa
     6421 caactaacgg cgatctcttt ttgtctttgt tttgaccaga cgttgtcgct aatcatcaag
     6481 ataattgttt aagagaccgg cgatcgactt gccgttcgtg gcgatgacat tgccgcatcg
     6541 gcgctgccgc cggcgcggcg cgcggccgca tcgggcgagc cggcgcgcga cagccggaca
     6601 cctttcggcc gcggcccggc gcgcgtataa ccggccgcaa tctggtcggt cgagcaacgg
     6661 gaatgggaga acgccgtgcg cgtttgtggt caggatacac tgaaggtgcg gcggtcgctc
     6721 gatgtcgatg gcgcacgcta cgactatttc tcgctgcccg aggccgagaa gcggctcggc
     6781 gagctgggcc ggctgccctt ctcgctcaag gtgctggtcg agaacctgct ccgcttcgag
     6841 gacggcagca cggtcaccgt ggcccatctc gaggccgctg ccgcctggct gaaggaccgg
     6901 cgcagcgacc aggagatcgc ctaccggccg gcccgcgtgc tgatgcagga cttcaccggc
     6961 gtgccggcgg tcgtcgacct cgcggcgatg cgcgaggcca tggtcgagct cggcggcgat
     7021 ccgcagagga tcaacccgct ctcgccctgc gacctcgtca tcgaccatag cgtccaggtc
     7081 gacagcttcg gcggcccggg cagcttcgag cgcaatgtcg agctggagta ccagcgcaac
     7141 ggcgagcgct acgccttcct ccgctggggc cagggagcct tcgacaattt ccgcgtggtc
     7201 ccgcccggca ccggcatctg ccaccaggtc aacctcgagc acctcgccca ggtcgtctgg
     7261 accggcaagg acggcaatgc cgagatcgcc tatcccgaca cgctggtcgg caccgacagc
     7321 cacaccacga tggtcaacgg cctcgccgtg ctgggctggg gggtcggcgg catcgaggcc
     7381 gaggcggcga tgctcggcca gccggtctcc atgctgctgc ccgaggtcgt cggcttcaag
     7441 ctcaccggcc ggctgcccga gggcaccacg gccaccgacc tcgtgctgac cgtcaccgag
     7501 atgctccgcg ccaagggcgt ggtcggcaag ttcgtcgagt tcttcggccc cggcgtcggc
     7561 cagctgccgc tcgccgaccg cgccaccatc gccaacatgg cgccggaata cggcgccacc
     7621 tgcggcttct tcccgatcga cgacgtgacg ctggagtacc tgcggctgac cggccgcgac
     7681 gaccgccgca tcgcgctggt cgaggcctat gccaaggcgc agggcatgtg gcgctcggac
     7741 gacctgcccg accccgtctt cacggccgtg ctcgagctcg acctcgccag ggtcgagccg
     7801 tcgctcgccg gcccgaagcg gccgcaggac cgggtggcgc tgggtgcggt caaggacagc
     7861 ttcgccaagg cgctgcccgg gctgctcggc aaggcgccgg ccgacaaggt ccagccgatc
     7921 gccggcatgg acgcggagct gcgccacggc gacgtcgtca tcgccgccat cacgagctgc
     7981 accaacacct cgaacccgag cgtcatgctg caggccggtc tggtggcgcg caaggcgcgc
     8041 gcgctcggcc tccagcccaa gccctgggtc aagacgtcgc tggcaccggg ctccaaggtg
     8101 gtcaccgact acctcgccgc cgccgggctg caggaggatt tcgacgccat gggcttcgac
     8161 ctcgtcggct atggctgcac cacctgcatc ggcaattcgg gcccgctgcc cgacgccgtc
     8221 gccgcggcgg tcgacgaggg cggcatcgtc gtcggcgccg tgctctccgg caaccgcaac
     8281 ttcgagggcc gcgtccaccc gcaggtcaag gccaactacc tggcctcgcc gcccctggtc
     8341 gtcgcctacg ccttcgccgg cacgctcacc cgcgacctga cgacggagcc gctcggcaag
     8401 ggcaaggacg gccagctcgt ctacctgaag gacatctggc cggaccaggc cgaggtcgcc
     8461 gaggtcgtgc gcaccgccgt cacggcggcg atgttcaaga agagctacag cgacgtcttc
     8521 aagggcgacg agcgctgggc cgccatcggc ggcggcgaca gcggcgtcac ctacgactgg
     8581 gacgacggca gcacctatgt ccgcctgccg ccctatttcg agggcatgga ggccgtgccg
     8641 ccggcatcgg tgcccgacat cgaggaggcc cgcgtgctcg ccatcctcgg cgattcgatc
     8701 accaccgacc acatcagccc ggccggctcc atcgcccagg cgagcccggc ggccgagtac
     8761 ctgcagagct tccagatcca gcgcaaggat ttcaactcct acggctcgcg gcgcggcaat
     8821 cacgaggtga tgatgcgcgg caccttcgcc aatatccgcc ttcggaacga gctggcggac
     8881 ggcaaggagg gcggctggac gaagctgatg cccgagggcg agccgatgtc gatctacgat
     8941 gccgcgatga agtacaagga gcgcggcacg ccgctcgtca tcttcgccgg caaggagtac
     9001 ggcacgggct cgtcacgcga ctgggcggcc aagggcacca accttctggg cgtcaaggcg
     9061 gtgatcgccg aaagcttcga gcgcatccac cgctcgaacc tggtcggcat gggcgttctg
     9121 ccgctcacct tcgagaacgg cgagagccgg tccagcctca agctcgacgg cagcgaggtc
     9181 gtcacgctcg agggcctcgc agccgggctg aagccgcgca tgaaggtggc ctgcaccatc
     9241 cgccggacgg acggcagcga gacgaagacc agcctgctct gccgtctcga taccggcgac
     9301 gaggtcgact actaccgcca cggcggcatc ctgcactacg tactgcgctc gctgctcggg
     9361 gcgacaccgg ccgccggccg cgcctgagcg ctcgacgcga agaggggcac cgccgtcggg
     9421 gcggtgcccc tctgcttgtc ggacccgacg tcagttgccg gcgcgatccg cctggtcgaa
     9481 gatgaagccg ccggcgccgc cgacgaccgc acccgccacg gtcgaagcca gcagcccgcc
     9541 ggtcagcacg ccgaccgccg cgcccgccgc ggcaccgacc gccgcaccgg tggcggcgcg
     9601 ctggccggtc ttgtcgtcga cgtcgagcac gccgcagccg gacaagaggc cgatcaacgc
     9661 cacgccggca accagccggc ttgccctgtt cctggtcgtc atcgaagttt ccccggacac
     9721 tgttgtgttc aattgcacgg ccacgtctcg gcggccgcct gcatcatccc atcgatcgcc
     9781 cgcgtgtcgg cctcctcggg atgcgcgccg gcccagccca cgaaggtgtc gcgcagcacg
     9841 gtcagcgtcg gctccggctc cgggcaggcg aggggctcga tcgcctcgat ccgcagcatc
     9901 tcggcataga gctgcccggc accgaggatg aagccgatgc agaaatgctg gtcgagctcg
     9961 gccggcgacg cgcagatcgc ctgcagctca ccggtcgtct gccgctcgaa cgggctggcc
    10021 tcggcgctcg ccgccgccgc cagcagcgcc gccccggcga gcaccgtcag tccctgacgc
    10081 attgttttct cctggattgt cgggcgcctc ggtcggaccc gccgcgtgcc gattgtatgc
    10141 gcagaattgc cggtcttcgc aatctctgtc atggcctcgg tcagggcagg gaacgggaga
    10201 acgcgacgtg gcgacggaca gggtggcact gatcacggct ggcggcagcg gcatgggtgc
    10261 ggatgccgcc cggcggctgg cggccgacgg ctttcggctc gccatcctct cctcgtccgg
    10321 caagggcgag gcgctggcgg ccgagctcgg cggcgtcggc gtcaccggct ccaaccgctc
    10381 gatcgacgac caccgccggc tggtggatct cgccatgcag cgctggggcc ggatcgacgt
    10441 gctggtcaac tcggccggcc acgggccgaa gggcccgatc ctcgcgatca gcgacgagga
    10501 ctggcatctg ggcatggagg tctatctcct gaacgtcatc cggccggcgc gcctggtcac
    10561 gccgatcatg gtggcgcagg gcggcggggc gatcatcaac atctcgacct tcgccacctt
    10621 cgagcccgac ccgctcttcc ccacctccgg cgtctttcgc gccggcctcg ccagcttcgc
    10681 caagctcttc gccaaccgct acgccgccga caacgtgcgc atgaacaaca tcctgccggg
    10741 cttcatcgac agcctgcccg agaccgagga tcgccgggcg cggatcccga tggggcgcta
    10801 cggcagggcc gaggaggtct ccgggcttat cgcctacctc gcctctgacg ctgccgccta
    10861 cgtcaccggc cagaacctgc ggatcgacgg cggcctcacc gcctccgtct aggctcccag
    10921 ggcatgagca agccgctcag gatcgcccgc gcccaggtca gagaggccgg cgccattgcc
    10981 cggctgatcg agccggcctt cgcccgcttc gtcgcccccg atctcggcga ggtcggccgc
    11041 gtcgcctttc gcctctacgt caccgagaag gcgcttcgcc gtcgtctcat ggacggtgcc
    11101 gtcggcttcc gcgccagcct gggagaggcg ctcgtcggct acgccgagct gcgcggtcgg
    11161 gacggccgga tcgacggcct cgaccacctg accctgctct tcgtggcggt cgacctgcag
    11221 ggccggggca tcgcccgccg tctcctggac gtgatcacgg gccacctggc ggcggcccgg
    11281 ccgccggtcg gcgatctcac cgtcaacgcc tcggcctatg ccgtcccgat ctaccaccgc
    11341 ctcggcttcc gggcgaccgg cgaggccggc gagttccagg gcgtcgtcgc gacgccgatg
    11401 cggctcgagc tcgacccggg cgggacgcaa gcggtttgat cgggcgcctc gccaccccat
    11461 ttctggaggg acgcgaagcg gatggctggt atgggcgagg actggcggct cctcatcttc
    11521 ctcggcaaga actgcgccct cggcatcgcc gccggctggc tgctgctggc ggccctgatc
    11581 cgcgccaatg tcggcgggct cggcgacctg ctcttcggca acgggctcga gctcaccgcc
    11641 ctcgtcctcg tggccgcggg cttcgccgtc accttcggcg ggctcgccat gggcacggcc
    11701 gtcttcctcc tgccgcgcga ctgagcgacc gcatcgccga gcgtttgcgt ctctgctaca
    11761 gacgtcgggt cgatccgaaa tcgggagaat ggcggatgag cggtgcgggc acggtgcgcg
    11821 aggcggtggc ggtgatggag gacgtggcgg cgctcgagct ggccgtcgag gcgctcgagg
    11881 aagccggctt cgccgacagc gacatcagcc tgctcgccgg ccacgggacg gtggaaggca
    11941 agctcgggcg gatgtaccgc cgggtcgagg aggccgagga cgaccccgcg gcaccccgca
    12001 ccgccttcgt ctcgacccgc accgtcgcca ggcgcgagga catggtggtg ggctcgatga
    12061 ccaccctgcc gaccctgatc gccgccggca ccgtcgtcgc ctcggccggt gccgtcgccg
    12121 ccgccatcgc cggcaccgcg atcaccggag ccctgatcgg cacggtgctg gcgcgctgga
    12181 tggacaagcg gcatgccgac tggctcgagg agcagctcga gcggggcggc atcctgctct
    12241 gggtgcgcac ccccgacgag gcggccgagc gcaaggcgct gcggatcctg ggcgagcacg
    12301 ccgcccacga catccacatc cacgagatcc cgaagcccgc ggactgagcg ggtcggcgct
    12361 cagtccccgg ccaccaccag ccgcgtgccg cacgattcga gcacggcggc gagctcgggc
    12421 ggcggggcgc ggtcggtgaa gaacgcgtcg atctggctca tgtcgccgag ccgcgccatc
    12481 gccggccggc cgatcttgct gtggtcggcg gccagataga ccttgcgcgc atggccgatg
    12541 atggtctgcg ccacccgcac ctcgcgatag tcgaagtcga gcagcgtgcc gtcgaggtcg
    12601 atggcgctga tgccgatcac cgcgatgtcc acccggaact gggcgatcga atcgatggtc
    12661 gcctcgccga cgatgccgcg gtcgcgggcg cgcaccaggc cgccggccac caccacctgg
    12721 aagtcctcgt tgccggccat cagctcggcg acgtgcaggt tgttggtgat cacgctcagg
    12781 ccccgatggt cgagcagcgc cttggcgatc tcctcgttgg tcgtgccgat attgatgcag
    12841 agcgaggcgt cgtcggggat gtgccgggcg acctcccgcg cgatccgccg cttcgcctcg
    12901 agatgcatca cctggcggct cgaataggcg atgttgtgca cgctggacgg caccgccgca
    12961 ccgccgtggt agcgctgcag cacgccctcg gcagcgagct cgttgatgtc gcggcggatg
    13021 gtctgcggcg tcacgccgaa ctgctgcgcc agcgcctcga tggtgacgaa gccctgacgc
    13081 tgcaccaggg ccacgatctc ggctcgccgc ccgacgaggt cgatcatcgg ccccgggtgc
    13141 gcacgcgacc gaccgcttcg gcccagccgt cgagccggcg gcggcgggtc gacgggtcga
    13201 gctgcggcgt gaaccggcca tcggcgcgcc agctctcggc gatgaaggcc tgcgacgggt
    13261 agaggccgat ggcgagcccg gcgagaaagg cggcaccgag cgcggtggtc tcgaccaccg
    13321 ccggccgctc caccgggcag tcgaggatgt ccgcgagata ctggcagacc cagtcattgg
    13381 cgaccatgcc gccatcgacc cgaagcgtct ccggcaacac accggcatcg gcggcctcga
    13441 tgtcgtcctt catcgccgcc atgagatcgg ccgtctggta gcagaccgat tcgagcgccg
    13501 ccctgacgat gtgccgaagg ccgctgtccc gggtcaggcc gaggatggcg gcgcgcgcgt
    13561 cgggatccca gtagggagca cccaggccgg tgaaggccgg caccaggtag accccgccgg
    13621 tgtccggcag gtcgcgggcc agggcctcgc tctcggccgc attgccgatc gcccgaagcc
    13681 cgtccctgac ccactggacg gcggccccgg cgatgaagat gctgccctcg atggcatagg
    13741 tcgtctttcc gtcgaggcga taggcgacgg tggtcaggag ccggtttctg gacggcacca
    13801 tccgcccgcc cgtgttgagc agcgcgaagc agcccgtgcc gtaggtgctc ttgagcatgc
    13861 cgggctcgaa gcaggcctgg ccgaaggtcg ccgcctgctg gtcgccggcc atgccggtga
    13921 ccgggatcgc ggcacccagg acggcggggt cggtggtgcc gaagaggccg ctgttgtcct
    13981 tgacctcggg cagcatcgag cgcgggatgc cgagccgctc gagcagctcc tcgtcccagt
    14041 cctgccggcc gatgtcgaag agcatggtgc gcgccgcgtt ggcggcgtcc gtcgcatgca
    14101 cctcgccgcc ggtcagccgc cagagcagaa agcagtcgac cgtgccgaag gcgagctcgc
    14161 cacgctcggc cctggcccgc gcccccggca cgttgtcgag aagccaggcc accttggtcg
    14221 cggagaaata ggcgtcgatg accagcccgg tcctggccgc caccgtctcg gtcagcccgt
    14281 cggcgaccag ctcgcgacag agcggcgccg tgcgccggtc ctgccagacg atggcattgg
    14341 cgatcgcccg gccgcttgcc cgctcccaga cgatcgccgt ctcgcgctgg ttggtgatgc
    14401 cgatggccgc gacccgagcg atcggcacgc ccgcccgctc caggacccac tcgcaggtgc
    14461 tgagcgtgtc ggcccagatg tgctcgggat cgtgctctac ccagccatcc gccggataga
    14521 gctgctgcag ctcgcgctgg gccgtggcga tcggccggcc ggtcgcatcg aacaggatcg
    14581 agcgggtgct cgtcgtgccc tggtcgatcg ccaggatgag atcggccgcc atcgcacact
    14641 ccgtcgccgt cttcgcaagg ccaatctagg agcctgtccg agtaatggcc actgcctgca
    14701 cgcgcccttc ctagctttgg gcgaggagac cggcccgccg cgatgcgggg ccatcggcag
    14761 ggccggtcga cgtggcccaa agccaggaat ggcgcgccct tcgggtcgtt ctctggcgg
//
LOCUS       DAOTFI010000118        14747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4888_length_14747_cov_7.898652, whole genome shotgun sequence.
ACCESSION   DAOTFI010000118 DAOTFI010000000
VERSION     DAOTFI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14747
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4888_length_14747_cov_7.898652"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="P5558_13095"
     CDS             complement(<1..510)
                     /locus_tag="P5558_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25296.1"
                     /translation="MFDLASSEQPATATLPEAWTAERYLDALHPRDGRGVVGGLTIQG
                     DQLRSHTWRRDILGGVTATVTEQEAYVAMNPYFAPRGGERRLAGINALWLDLDTYRVP
                     GLATLPRHEVPERILSALREAELPSPSMLLDSGRGYYCIWLVSGASPAALCCDPRFLL
                     HLRLESGLRE"
     gene            complement(1135..1481)
                     /locus_tag="P5558_13100"
                     /pseudo
     CDS             complement(1135..1481)
                     /locus_tag="P5558_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766426.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1853..2503)
                     /locus_tag="P5558_13105"
     CDS             complement(1853..2503)
                     /locus_tag="P5558_13105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRY25297.1"
                     /translation="MAYGSSCFMVSTRLGRFGPTVSRFAFRGVRRPATATAERALLRA
                     DHANPDQRFQNVRAQPANERFRAGLRKPPPAPLPLRDFEQRIADLPRTTEAERLTVQR
                     IGQDIFRDRLMDYWQGRCPLTWISDPALLRASHIIPWSECESDAERLDVHSGLLLSAL
                     WDAAFDRALVTLDDQGRPEYSSRLTEQARAELRWSSSVALTDEHRRRLARHGERAQ"
     gene            2636..3844
                     /locus_tag="P5558_13110"
     CDS             2636..3844
                     /locus_tag="P5558_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25298.1"
                     /translation="MMHRRSFLSCSSLAAVSLAAPGLFTRAHAAGDALKIGIPVPISG
                     AFAANGQFATMGALMAAEEVTQAIGREVSTIDLDTEGKPATAVRKVQDAMAQDGVGLF
                     AGGILSSESLAMGKEVARGNAVLMTTAGADEITGVDCNRGTFRWSVPTYGAINETVRP
                     LIDLLPNAKRWYTITPQYVFGDGLLNAAKDVFAEKGVEHLGNSYHSLTDKEFSGYLTN
                     AIAAQPDVLLLLNFGAQSSDTLRQAVSFGLKERMTIVVAWASGLEQFEGLGADICDGV
                     YFGAQYWHAADAPANRELVAKVQEKHGINPNYSFAGSYLCSKLLLDAAVKVDSTDGGA
                     VAGVLEGLTYDGLTGQEEIRAADHQVIKEYYLLKGKAKDAMADKDDYADIVSSGKSFL
                     SPEEAGCKMS"
     gene            3909..4763
                     /locus_tag="P5558_13115"
     CDS             3909..4763
                     /locus_tag="P5558_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY25299.1"
                     /translation="MIYLFQVLNGIGIGMLYFLLAVGLSVIFGLLQFVNFAHGAFYLL
                     GAYLTYDLTSRGLSFWAAMPIATLIVAGAAALIEALLLHRIYKLPHSFHILVTVGLAL
                     MIQELTIIVWGPLGGSVAPPAGLTGVVMLGDFIYPQYRLFTIAFTAVLAALLWWLLEG
                     TRLGAVVRGGSESSDNMALLGYNTLRINTAVFGFGAGLAGLAGALAAPIRGVEPFMGI
                     EALSVAFVVVVIGGMGSYTGALVAGLLVGVTQSVMAAIWPEGARLMIYIGMALVIALM
                     PRGLLGRA"
     gene            4765..5745
                     /locus_tag="P5558_13120"
     CDS             4765..5745
                     /locus_tag="P5558_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY25300.1"
                     /translation="MNVVNKPVVQFGLALLTVAVLPLTLSSGILASEILIFAMVVAAC
                     NLLLGYTGLLSFGQGIFFGVGSYVAGIFLTRFDLPVPVVLAASLVLGAAIAALVGWLS
                     IRRQGVYFVMLTLAFAQMFYFLAYTFSGLTGGDNGLLDIPRPTVGGAALDGPWSYYVY
                     VAVCFVVVFGLLLVATQSTFGRTLIAIRENESRAAAIGFPTRLFKIEAFAISGAVTAF
                     AGALHAMLIGVAPLSNIEYHISELILIMTIIGGSSSLFGSVLGAGFYLLLADTLSAIW
                     PRWLLLLGLCLVLVALFLQRGIWGLVERLASAVTGGPGPAAPAATSGEEA"
     gene            5747..6514
                     /locus_tag="P5558_13125"
     CDS             5747..6514
                     /locus_tag="P5558_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25301.1"
                     /translation="MSEIALETHGLGVKYGKFVALADVDLSIRKNSTHSIIGPNGAGK
                     TTLFHALTGKVRASTGRIVLDGHDVTATSDDDRVRLGIARSFQVTSLFPNLAVRENLR
                     LAAQGKSPWQALEPWRRAESNIEALTIADEVLTRLSLTSVGNRAAGELSHGQQRRLEV
                     GMAMASRPRVILLDEPTSGMGIDDIETMKSLIRDLSRDHTVLFIEHNMGIVMNISDTV
                     TVMRLGRKLVEGPPAVVRDDPEVQRAYLGNMITGDIA"
     gene            6511..7218
                     /locus_tag="P5558_13130"
     CDS             6511..7218
                     /locus_tag="P5558_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25302.1"
                     /translation="MTPALRVDDIHSYYGKSHILQGVSLEVGQGEIITLLGRNGAGKS
                     TTLKSIVGLVTPRSGRVEIAGRDVTGQPAYRIAAAGLSLVPEDRGIFQLLTVEENLRI
                     AQSRGSQWSLADVYGIFPRLEERRKNGGGQLSGGEQQMLSIARALLNGPKVLMLDEPV
                     EGLAPIIVEEIVTQILKIRDAGVPILLVEQNLAVCNKVADRHYILELGRIAHHATAEE
                     FRSDPDTIDRYLGVKAA"
     gene            7241..8500
                     /locus_tag="P5558_13135"
     CDS             7241..8500
                     /locus_tag="P5558_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRY25303.1"
                     /translation="MSSIAINADRLWNSLMETARFGGTADGGIARLTLSDEDRQVRDW
                     LAARCRELGMTVTVDEVGNMFALRPGRRDDLLPIAMGSHLDTQPTGGRFDGVLGVLSA
                     LEVVRSLDDAGITTEAPLMVVNWTNEEGSRFSPAMLGSGVYAGVYTPEHCAARVDVGG
                     VAFGTALERIGYRGDAAAGSVRLGALFEVHIEQGPILDAEGIAIGVVQGVQGMRWFDL
                     ILTGVAAHTGSTPMSMRTDALLGMARVIAGIEAIALRHPDSVGSIGFVEVQPNSHNVI
                     AGQVRATIDLRHPDDAVLDRVEAETEALVAEAARERGLEATLTPVARTAAVVFDAECI
                     ASVRAAAQAAGLTTRDIVSGAGHDAVHIARILPTTMIFVPCEKGLSHNPAESSTEVEC
                     AAGAQVLLDAVLDYDRRYLARLIREPA"
     gene            8497..10023
                     /locus_tag="P5558_13140"
     CDS             8497..10023
                     /locus_tag="P5558_13140"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRY25304.1"
                     /translation="MTLRLVVARLNHETNTFSPVPTPLAAFNPKWGEEAAAAAADSPT
                     ALGAFHAFGARIGAELSIPLVAHANPSGPVDDAAFEAMADAILDAVRRGCDGILLDLH
                     GAMVTKSHDDGEGELLARVRRAAPDVPLGVALDLHGNITQRMLDNCDAIVGFKTYPHI
                     DMVETGEHLVRIMEPILAGGPRPAMALCHPPMLAATLCMNTAVECAMTDLVRRAREAE
                     ARDGVHAVTVFGGFAIADLAETGLSVVAVAERAEAAQHAACELGAAAWRRREEFVYRE
                     ERLAESIARAGRLADGTAPVLLLDHGDNCNSGGSCDVMDVLEASLAAGHQRIVVGPIA
                     DPGTVAQMFESGVGATVTVALGNKTPASDFPPPRAPLAITGRVRALGDGSYRATGPIY
                     HGELLSMGRAAALETDRAVIVVCEQPHEPLDLGVFSCLGIDLSGTQFLILKSRMYCRP
                     VFEPMSKAVVECASAGVTSSNYDLFAFKKLARPIYPLNADLAWEPEGQSCASTTGSRQ
                     "
     gene            9993..10769
                     /locus_tag="P5558_13145"
     CDS             9993..10769
                     /locus_tag="P5558_13145"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY25305.1"
                     /translation="MRQYNRFTAVDGRLTRIKLSDQVAEDIRHWIARENLRTGDRLPN
                     ERALMEHYGCSKGTIREALKALEVEGLVRMLSGPNGGPEVQAASIDIVTQQFRQYLHF
                     QKVSFRQVYELRRVIEVILATNVVGKLTEDHFRRLAENIRVCEEATAGGDWSVGRRAE
                     TEFHDILCEASDNFILVFMCRFLNGMLRDLVKFRSNSMREHEAFGEHNIDSHRKLLAA
                     LRAGDAAAAADIMAEHMLCAEGFMSRLDASFQSDLLSRQS"
     gene            complement(10786..11352)
                     /locus_tag="P5558_13150"
     CDS             complement(10786..11352)
                     /locus_tag="P5558_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY25306.1"
                     /translation="MQTPSATQVDIGGRLRHLRMLHGLSQRELAKRAGVTNSTISLIE
                     SNGTNPSVGALKRILDGIPVGLAEFFAFEPERPRKAFYAAEELVEIGKGGVSYRQVGE
                     TMFGRALQILKECYQPGADTGKVPLSHDGEEGGVVISGRLEVTVEDERRILGPGDAYC
                     FESRRPHRFRCVGAEPCEVISACTPPSF"
     gene            11479..12810
                     /locus_tag="P5558_13155"
     CDS             11479..12810
                     /locus_tag="P5558_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRY25307.1"
                     /translation="MPLDRPNTPKLDSYWMPFTANRQFKAAPRLLVAADGMHYTSADG
                     HQVLDGTAGLWCCNAGHGRRWITEAVSRQLATLDYAPSFNMGHPVAFDFAERLAAVAP
                     GPDGAKLDRIFFTGSGSESVDTALKIAIAYQRAIGQGTRTRVIGRERGYHGVGFGGIS
                     VGGLVNNRRVFPQIPADHLRHTHDLARNAFVKGQPEHGAELADDLQRLVDLHGAETIA
                     AVIVEPVAGSTGVLVPPKGYLERLREIATKHGILLIFDEVITGFGRLGAPFATDFFGV
                     TPDLVTTAKGLTNGIIPMGAVFASRAVHDAMMTGPENAIEFFHGYTYSGHPVACAAGL
                     AALDIYEGDGLLTHAAELQAKWDAAIHSLKGASNVIDIRTIGLIAGIELGSREGAPGT
                     RAYDVFVDCLEKGLLIRVTGDIIALSPPLIVEEGQIDEIVSTLGDALNRAA"
     gene            12841..14235
                     /locus_tag="P5558_13160"
     CDS             12841..14235
                     /locus_tag="P5558_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY25308.1"
                     /translation="MLDAVLRRAGAVLEIDLGAIRENYRILRAKARGGCAAVVKADAY
                     GLGATVVAPALHAEGARSFFVAHLDEALALQPALPVGTQILVLNGLPPGAEADCAAAG
                     IVPVLNSLGQVDAWAAFARLKGRTLPAAIQVDSGMSRMGLPEHDLARPGAALYGIAPV
                     AGEANPMRPVVRLRGRIVQVRDIPAGAHVDYGASWCAAQPSRIATVSVGYADGYLRSL
                     SHRAGAHVGGTAVPLVGIVSMDSITFDVTDAPDARAGGFVDLIGPENPVDAAAEAGGT
                     IGYGILTSLGSYSPLILKEHGIDLPVYPVKGYSLTVPITEPSGAPESTVMDEKHKVAI
                     TRLGDRIRVGGMAELDGYSTDLHPNRRATLEHVVSDLYPTGGDAKAGTFWAGHRPMTP
                     DGPPMIGRTKYPNLWLNTGHGTLGWTMACGSGAVLADLVSGRTPAIDARALGIERYAA
                     AAKALSNDSKEVPV"
     gene            14232..14660
                     /locus_tag="P5558_13165"
     CDS             14232..14660
                     /locus_tag="P5558_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRY25309.1"
                     /translation="MTPINASGAPAAIGPYCHAVKVGDLLFTSGQIPLDLAGAMPEGV
                     EAQTDQVFDNLAAALKETGASLDQVVKATVFVTDLGDFAKLNAVYERRFGAHKPARST
                     VQVAPCRAAPRSKSNSSCNCATPEPKLNPVPQATAMEERG"
BASE COUNT         2346 a         5164 c         4864 g         2373 t
ORIGIN      
        1 ctctctcagg ccggactcta acctcagatg gaggagaaag cgggggtcac agcacagcgc
       61 cgccggcgac gcgccgctga cgagccagat gcagtagtat ccccggccgc tgtcgagcag
      121 catagagggg gacggcaact cagcttcgcg caatgccgac aggatacgct ccggcacttc
      181 gtgccgtggc agcgtcgcca ggcccggaac gcggtaggtg tcgaggtcga gccagagggc
      241 gttgatgcct gcgagtcgcc gctcgccccc gcgtggcgcg aagtacgggt tcatcgcgac
      301 ataggcctcc tgctccgtca ccgtcgccgt gacgccacca agaatatcgc gccgccaagt
      361 atgtgaccgg agctggtctc cctggatggt cagcccaccc accacaccac ggccgtcgcg
      421 cgggtgcagg gcgtcgaggt atcgctccgc cgtccaggct tctggaagcg tcgcggtcgc
      481 gggttgctcg gaggaggcga gatcgaacat ggcagggaca aagggcccga cgataccggt
      541 ttcaaacgcc gctggtgatt actaccgccg gtcgagagat ccgttgacga gcacgatcgc
      601 ctacacctga gacttgagac gtgccttggg cggagcaacg agctaaggga cgatgcatgg
      661 cgtcttcgag caaatcgcgc cggggccaga cttcgcggac acgggccgga tgttgccgac
      721 gtggtctcgg tctcgcgcta ttgtccgaac gcgtcgaatt tcgtcttccg cctggacgag
      781 cacgtccaca agcggctgct ctaccgcggc gaggatacag gcgcgccatc cagcggccag
      841 acgacaggct ggcagagaac ctccgcctct cggtccgcgg ctggtcgaag acgcgccgca
      901 cgtcttctcc aaggagtcaa tggcccggga gtggcggcgc ggccttccgt ccgatcggtt
      961 gcgcgaggtg ctgaactatg tcgagtgcct cgccggcatt cggaccaccc tatccacgtc
     1021 tcggggtgcg agcggaaggc gcggaacgcc gatcgtcacc atcgccgacg ccgacgccga
     1081 cgccgacgcc gaaggacagg taacaaaaga cgcgtcctat tcgggaatgt tgcagatcag
     1141 gcccggcagc tccgcgccgt cgtggcgggc ggagtcggtg gcggtccgtg tccggatcag
     1201 cccgggccgg cgatcgatgc cgacatggtt cttgtagccg cagaccggga tcgcgatggc
     1261 gatccgcggt gccccgtcaa ggccgggctt caccttgcct gcttcaccgt ccagcgggcg
     1321 tcattcacca atgtctctcg gcccattggc gttccattgg cgaattcctt ttgccgcagc
     1381 ttcgccggcc tgcgggccca atcctcgggg atgcggcctt cgcgcagcgc ctgcttctcg
     1441 ccttccgtgt tgcgctgctt cggggcggcg accacaggcg ccattccgca atggtcctgg
     1501 ctttgatctc ctttcgtctt tcgaggatgg tttcggccct gccgaggcaa acgtcggcga
     1561 ggtgaggttg ctcagggtct cgtggtagcg gtggtggtgg ccatctcgat ccgtcgaagg
     1621 aaggggacgc cggtaacatc gttccggtgt gcccctcctg caacaaatgg gcccaggccc
     1681 gcgacgccgc cttcgatttc ggcccgagga cgctggtgcc ccggccgatc atgctctcta
     1741 ccgcggagcg gcggcgcatt gaaggctgac agatcggcct tcgatgagcg ttcgccgaag
     1801 atcggaaggc tgaacgcgat ccccgccggg gtccgcggcg ctacatcatc cttcactgcg
     1861 ctcgctctcc atggcgagcg agccgccggc ggtgctcgtc cgttagcgca acggaggaac
     1921 tccagcggag ttcggcgcgc gcttgctcgg tgaggcggga cgagtactcg ggcctacctt
     1981 ggtcgtcgag ggtcacgagc gcgcggtcga atgcggcatc ccagagcgcc gatagcagca
     2041 ggccgctatg cacgtcgagc cgctctgcat cgctctcgca ctcgctccat gggatgatgt
     2101 gcgaagcgcg caacagagcc gggtccgaga tccaggtcag ggggcaacga ccctgccagt
     2161 agtccatcag acggtcgcga aagatgtcct gcccaatcct ctgcacggtc agccgttcgg
     2221 cctcggtcgt gcggggcaga tccgcgatgc gctgctcgaa atcccgcagc ggaagcggtg
     2281 caggcggcgg ctttcgaagc ccggcgcgaa aacgttcgtt ggcaggctgc gcgcgaacgt
     2341 tctggaagcg ctgatctgga ttagcgtgat cggcccggag taacgctcgc tctgcagtcg
     2401 ccgtggcggg tctccggacc ccacggaacg cgaatcgact gactgtcggg ccgaatcgtc
     2461 caaggcgcgt cgataccata aagcaggagg acccgtaagc caagactctg gtttcgcttc
     2521 tgtttcccgg tcgcagctca aaaaggcgga ccgctcgaca aactatcata ttaatgcgta
     2581 tagttaggat ggttgccaag gcggcagctc tcaaaccgac agggtaaaac catccatgat
     2641 gcaccggcgg agtttcctca gctgctcgtc ccttgctgct gtttccctcg cagcgcccgg
     2701 cctgttcacg cgcgcccatg cggcgggtga tgcgctcaag atcggcatcc ccgtgccgat
     2761 ctcgggagcc ttcgccgcca acggccagtt cgcgacgatg ggcgcgctga tggctgcgga
     2821 agaggtgacg caggcgatcg ggcgcgaggt ctcgacgatc gatctcgaca ccgaaggcaa
     2881 gcccgccacc gccgtgcgca aggtgcagga cgcgatggcc caggacgggg tcgggctctt
     2941 cgccggcggc atcttgtcgt cggaatcgct cgccatgggc aaggaggtcg cccgcggcaa
     3001 cgccgtgctg atgacgacgg ccggcgccga cgagatcacc ggcgtcgact gcaaccgcgg
     3061 cacctttcgc tggagcgtgc cgacctatgg cgcgatcaac gagacggttc gcccgctcat
     3121 cgacctgctg ccgaacgcca agcgctggta caccatcacg ccgcagtatg tcttcggcga
     3181 cgggctgctc aatgccgcca aggacgtctt cgcggagaag ggcgtcgagc atctcggcaa
     3241 cagctaccac tcgctgaccg acaaggagtt cagcggctat ctgactaacg cgatcgctgc
     3301 ccagcccgac gtgctgctgc tgctgaactt cggagcgcaa tcctccgaca ccctgcgaca
     3361 ggccgtgagc ttcggcctga aggagcgaat gaccatcgtg gtcgcctggg cctccgggct
     3421 cgagcagttc gagggcctcg gcgccgacat ctgcgacggg gtctacttcg gcgcccagta
     3481 ctggcacgcc gccgatgcgc cggcgaaccg cgaactggtg gcgaaggtgc aggagaagca
     3541 cgggataaat cccaactact ccttcgcggg gtcatacctc tgcagcaagc tgctcctgga
     3601 tgcggcggtg aaggtcgact cgaccgacgg cggggcggtg gccggcgtcc tcgagggact
     3661 gacctacgac ggcctgaccg gccaggagga aatccgggcg gccgaccacc aggttatcaa
     3721 ggaatactat ctcctgaagg gcaaggcgaa ggacgcgatg gccgacaagg acgactacgc
     3781 cgacatcgtg agttccggca agtccttcct gtcgccggag gaagccggct gcaagatgag
     3841 ctgagctgcc gtggcgccgc ggcatttacg cggcgccgca ccctgacatc agaactcagg
     3901 agattgtcgt gatctatctc ttccaagtgc tcaacggcat cggaatcggg atgctgtact
     3961 tcctgctggc ggtcgggctg agcgtgatct tcggtctcct gcagttcgtc aacttcgccc
     4021 atggcgcctt ctacctgctc ggcgcctatc tcacctacga tctcaccagc cgcgggctca
     4081 gcttctgggc ggcgatgccg atcgccacgc tgatcgtggc gggcgccgcc gcgctcatcg
     4141 aggccctgct gctgcaccgc atctacaagc tgccgcactc gttccacatc ctcgtcaccg
     4201 tcggccttgc gctgatgatc caggaactga cgatcatcgt ttgggggccc ctgggcggca
     4261 gcgtcgcgcc gccggccggg ctgaccggcg tggtgatgct cggcgacttc atctatccgc
     4321 agtaccgcct gttcacgatc gccttcaccg cggtcctcgc tgccctcctc tggtggctgc
     4381 tcgaaggcac gcgccttggc gccgtcgtcc gcggcggttc ggaatcgtcc gacaatatgg
     4441 cgctgctcgg ctacaacacg ctgcggatca acaccgccgt cttcgggttc ggcgccgggc
     4501 tcgccgggct cgccggcgca ctggcggcgc cgatccgcgg ggtcgagccg ttcatgggca
     4561 tcgaggccct ctccgtcgcc ttcgtggtcg tcgtgatcgg cggcatgggc agctacaccg
     4621 gggcgctcgt cgccgggctg ctggtcggcg tgacccagag cgtcatggcg gcgatctggc
     4681 ccgagggcgc gcggctgatg atctacatcg gcatggccct cgtcatcgcg ctgatgccgc
     4741 gcggcctgct cgggagggcc tgagatgaac gtcgtcaaca agccagtcgt ccagttcgga
     4801 ctcgccctgc tgaccgtggc ggtgctgccg ctgacgctct cgtcgggcat cctcgccagc
     4861 gagatcctga tcttcgcgat ggtggtcgcg gcctgcaacc tgcttctcgg ctacaccgga
     4921 ctgctctcct tcgggcaggg catcttcttc ggcgtcggga gctatgtcgc cggcatcttc
     4981 ctgacccgct tcgaccttcc ggtcccggtg gtgctcgccg cctcgctcgt tctcggcgcc
     5041 gcgatcgcgg cgctggtcgg gtggctctcg atccgccgcc agggcgtcta cttcgtgatg
     5101 ctgaccctcg ccttcgcgca gatgttctac ttcctcgcct ataccttcag cggactgaca
     5161 ggcggcgaca acggtctgct ggacatcccg cgaccgacgg tcggcggggc ggcgctcgac
     5221 ggtccgtgga gctactacgt ctatgtcgcg gtctgcttcg tggtggtctt cgggctgctg
     5281 ctggtggcca cgcagtcgac cttcgggcgc acgctgatcg ccatccgcga gaacgagagc
     5341 cgggccgcgg ccatcggctt cccgacccgc ctcttcaaga tcgaggcctt cgcgatctcg
     5401 ggcgcggtca cggccttcgc tggcgcgctg cacgcgatgc tgatcggcgt cgctccgctc
     5461 tcgaacatcg agtaccacat cagcgaactc atcctgatca tgacgatcat cggcggcagc
     5521 tccagtctct tcggctcggt gctcggcgcg ggattctacc tgctgctggc cgacacgctg
     5581 tcggcgatct ggccgcgctg gctgctgctg ctcgggctct gcctcgtcct cgtggcgctc
     5641 ttcctgcaac gcggcatctg gggcctggtc gaacggctgg cgagcgcggt gaccggtggc
     5701 ccaggccccg ccgccccggc cgcgacctcg ggcgaggagg cctgacatga gcgagatcgc
     5761 actcgagacc cacggcctcg gcgtcaagta cggcaagttc gtcgccctgg ccgacgtgga
     5821 cctgtccatc cgcaagaaca gcacccactc gatcatcggg ccgaacggcg ccggcaagac
     5881 cacgctcttc cacgcgctga ccggcaaggt cagggcctcc accggacgga tcgtgctcga
     5941 cggccacgac gtcaccgcga cgtcggacga cgaccgggtc cgcctcggca tcgcgcggtc
     6001 cttccaggtg acgagcctgt tcccgaacct cgccgtgcgg gaaaacctgc gcctcgcggc
     6061 gcagggcaag tcgccctggc aggcgctgga gccctggcgg cgggccgaga gcaacatcga
     6121 ggccctgacg atcgccgacg aggtgctcac ccggctctcg ctcacgtccg tcggcaaccg
     6181 cgccgcgggc gaactgtcgc atggccagca gcgccggctc gaggtcggca tggcgatggc
     6241 ctcgcggccg cgggtgatcc tgctcgacga gccgacctcc ggcatgggga tcgacgacat
     6301 cgagacgatg aagtcgctga tccgagacct cagccgcgac cacaccgtgc tcttcatcga
     6361 gcacaacatg gggatcgtga tgaacatctc ggacacggtt accgtgatgc ggcttggccg
     6421 caagctcgtc gaagggccgc cggccgtggt ccgcgacgac cccgaggtgc agcgcgccta
     6481 cctgggcaac atgatcaccg gagacatcgc atgaccccgg ccctgcgcgt cgacgacatc
     6541 cacagctact acggcaagag ccacatcctg cagggcgtgt cgctcgaggt cggacagggg
     6601 gagatcatta ccctgctcgg ccgcaacggg gccggcaaga gcacgacgct gaagagcatc
     6661 gtcgggctcg tcaccccgcg ctccggccgc gtcgaaatcg ccgggcgcga cgtgaccggc
     6721 cagcccgcgt accgcatcgc cgccgccggg ctgtcgctcg tgccggaaga ccggggcatc
     6781 ttccagctcc tcaccgtcga ggagaacctg cgcattgccc agagccgcgg ctcgcagtgg
     6841 tcgctggccg acgtctacgg gatcttcccg cggctcgagg agcggcggaa gaacggcggc
     6901 ggccagctct cgggcggcga gcagcagatg ctgtcgatcg cgcgcgcgct tctcaacggc
     6961 ccgaaggtgc tgatgctcga cgagccggtc gaaggcctcg cgccgatcat cgtcgaggag
     7021 atcgtgaccc agatcctgaa gatccgcgac gccggcgtcc cgatcctgct ggtcgagcag
     7081 aaccttgcgg tctgcaacaa agtggccgac cgccactaca tcctcgagct cggccgcatc
     7141 gcccaccatg caaccgccga ggagttccga tccgatcccg acaccatcga ccgctatctc
     7201 ggcgtgaagg cggcctgacc ccgtgcgtga ggagacgaca atgtcaagca tcgccattaa
     7261 cgccgaccgg ctgtggaaca gcctgatgga gacggcccgt ttcggcggca ccgccgacgg
     7321 cggcatcgcc cgcctgacgc tcagtgacga ggaccgccaa gtccgcgact ggctggccgc
     7381 acgctgccgc gagctcggca tgaccgttac cgtcgacgag gtcggcaaca tgttcgcgct
     7441 gcggccaggc cggcgcgacg acctgctgcc gatcgccatg ggcagccacc tcgacaccca
     7501 gccgaccggc ggtaggtttg acggcgtgct cggggtcctc tcggcgctcg aggtcgtgcg
     7561 gagcctcgat gatgccggga tcaccaccga ggcgccgctg atggtggtca actggaccaa
     7621 cgaggagggc tcgcgcttct caccggcgat gctgggatcg ggggtctacg ccggggtcta
     7681 cacccccgag cactgcgccg cgcgcgtcga cgtaggcggc gtggccttcg ggacggcgct
     7741 ggagcgaatc ggctatcgcg gcgacgccgc ggccggatcg gtccggctcg gggcgctctt
     7801 cgaggtgcac atcgagcagg gtccgatcct cgacgccgag ggcatcgcga tcggcgtggt
     7861 gcagggcgtc caagggatgc gctggttcga cctcatactg accggcgtcg ccgcgcacac
     7921 cggctcgacc ccgatgtcga tgcggaccga cgccctcctc ggcatggcgc gcgtgatcgc
     7981 tgggatcgag gcgatcgccc tgcggcaccc ggactcggtc ggctcgatcg gcttcgtcga
     8041 ggtccagccc aactcgcaca acgtgatcgc ggggcaggtc cgggcgacga tcgacctgcg
     8101 tcatcccgac gacgcggtgc tcgaccgggt ggaggccgag accgaggcgc tggtggcgga
     8161 ggccgcgcgc gagcgcgggc tcgaggcgac cctcaccccg gtcgcccgca cggcggccgt
     8221 cgtcttcgac gccgagtgca tcgcgtcggt gcgggcggcg gcgcaggcag ccggcctgac
     8281 gacccgcgac atcgtctccg gcgccggcca cgacgccgtc cacatcgcgc gcatcctgcc
     8341 gacgacgatg atcttcgtgc cctgcgagaa ggggctcagc cacaacccgg ccgagtcctc
     8401 gaccgaggtg gaatgcgccg ccggcgccca ggtgctgctc gacgccgtcc tcgactacga
     8461 ccggcgctac ctcgcgcgac tgataaggga accggcatga ccctgcgcct cgtcgtcgcg
     8521 cggctgaacc acgagaccaa caccttctcg ccggtgccta cgccgctcgc ggccttcaac
     8581 ccaaaatggg gcgaggaagc agccgcagcc gcagccgact ctcccaccgc tctcggggcc
     8641 ttccacgcct tcggcgcgcg catcggcgca gagctctcca tccccctcgt cgcccacgcc
     8701 aatccgtccg ggccggtcga cgacgcggcg ttcgaagcga tggccgatgc gatcctcgac
     8761 gcggtgcgca ggggctgcga cggcatcctg ctcgacctgc acggcgccat ggtcacgaag
     8821 agccacgacg acggcgaggg cgagctcctc gcccgggtcc gccgcgccgc gcccgacgtg
     8881 cccctcggcg tggcgctcga cctgcacggc aacatcaccc agcggatgct cgacaactgc
     8941 gacgcgatcg tcggcttcaa gacctatccg cacatcgaca tggtggagac cggcgagcat
     9001 ctcgtccgga tcatggagcc gatcctcgcc ggcggccccc gccccgcgat ggcgctctgc
     9061 catccaccga tgctggccgc gacgctctgc atgaacactg cggtcgagtg cgcgatgacc
     9121 gacctcgtcc gccgagcccg cgaggcggag gcccgcgacg gggtccacgc cgtgacggtc
     9181 ttcggcggct tcgccatcgc ggatctcgcc gagaccgggc tgtcggtcgt cgccgtcgcc
     9241 gaacgggccg aggcggcgca gcacgcagcg tgcgagctcg gcgccgcagc ctggcggcgg
     9301 cgggaagagt tcgtttaccg cgaggagcgg ctcgcggagt cgatcgcccg cgccggccgg
     9361 ctggcggatg gaaccgcgcc ggtgctgctc ctcgaccatg gcgacaactg caactccggc
     9421 ggcagctgcg acgtcatgga cgtcctcgag gcgtccctcg cggccggcca ccagcggatc
     9481 gtcgtcggac ccatcgccga tcccgggacc gtcgcgcaga tgttcgagtc gggtgtgggg
     9541 gctacggtga cggtcgcgct cggcaacaag acgccggcct ccgactttcc gccgccccgc
     9601 gcaccgcttg cgatcaccgg gcgcgtgcgc gcactcgggg acggcagcta tcgggcaacc
     9661 gggccgatct atcacggcga gctcctctcg atgggccgcg ccgctgctct cgagaccgac
     9721 cgcgcggtca tcgtggtgtg cgagcagccg cacgagccgc tcgacctcgg cgtcttctcg
     9781 tgtctcggca tcgacctttc cggaacgcag ttcctcatcc tgaagtcgcg gatgtactgc
     9841 cgtccggtgt tcgagccgat gtcaaaagcg gtggtggagt gcgcgagtgc cggcgtaacg
     9901 agttcgaact acgatctatt tgctttcaag aagctggctc ggccgatata tccactgaac
     9961 gccgatctcg cctgggagcc ggaaggtcaa tcatgcgcca gtacaacagg ttcacggcag
    10021 tagacggaag gcttacccgg atcaagctgt ccgaccaggt cgcggaggac atccggcact
    10081 ggatcgccag ggaaaacctc cgaacaggcg accggctccc aaacgagcgc gcactaatgg
    10141 agcactacgg ctgctcgaag ggcacgatcc gcgaggcgct gaaggcgctc gaggtcgaag
    10201 gcctggtgcg gatgctgtcc gggccgaacg gtggccccga ggtgcaggcc gcctcgatcg
    10261 acatcgtcac gcagcagttc cgccagtatc tgcatttcca gaaggtgagc ttccggcagg
    10321 tctacgagct gcggcgggtg atcgaggtca tcctcgccac caacgtcgtg ggcaagctga
    10381 ccgaggacca tttccgccgc ctcgccgaga acatccgggt ctgcgaagaa gcgacggccg
    10441 gcggcgactg gagcgtgggg cggcgggcgg agaccgagtt ccacgacatc ctgtgcgagg
    10501 cgtcggacaa cttcattttg gtgttcatgt gccgcttcct gaacggcatg ctgcgtgacc
    10561 tggtgaagtt ccgttccaat agcatgcgtg agcacgaagc gtttggagag cacaacatag
    10621 acagccacag gaagctcctt gcagcgctcc gcgccggaga tgcggccgcc gcggccgaca
    10681 tcatggcgga gcacatgctc tgcgctgagg gattcatgag caggttggac gcgtccttcc
    10741 agtccgatct gctcagccgg caatcctgac aggacgcggg caccctcaga agctcggcgg
    10801 cgtgcaggcg ctgatcacct cgcagggctc ggcgccgacg catcggaacc tgtgcggtcg
    10861 gcggctttcg aagcagtagg cgtcgcccgg gccgaggatg cgccgctcgt cctccacggt
    10921 aacttcgagc cggccggaaa tgacgacgcc gccttcctcg ccgtcatggc tcaggggcac
    10981 ctttccggtg tcggcgccgg gctggtagca ttccttaagg atctgcagcg cgcggccgaa
    11041 catcgtctcg ccgacttgcc ggtaggagac gccgccctta ccgatctcga ccagttcctc
    11101 ggcagcgtag aaggccttgc gcggccgctc cggctcgaag gcgaagaact ccgcaagccc
    11161 cacagggatg ccgtcgagga tgcgcttcaa tgcccccacc gacgggttcg tgccgttcga
    11221 ttcgatcagc gagatcgttg agttggtgac gccggcgcgc ttcgcgagct cgcgctgtga
    11281 gagcccgtgg agcatgcgga gatgccgcag ccggccgccg atgtcgacct gagtcgcgga
    11341 gggtgtttgc actgttcggt attcccgcaa tgtctgtcat tgttccgtta tattacagca
    11401 tcttatcgtc gaacagaaaa ggacttgttt cgcaatctcc gacatgactc ctgccggcat
    11461 cagccacgga gatccgccat gccgctcgac cgcccgaaca cgccgaagct cgacagctac
    11521 tggatgccct tcactgccaa ccgccagttc aaggcggccc cacggctgct cgtcgccgcc
    11581 gatgggatgc actacacctc ggccgacggt caccaggtgc tcgacggcac agccggcctc
    11641 tggtgctgca acgccggcca cgggcgccgg tggatcaccg aagcggtcag ccgccagctc
    11701 gccacgctcg attacgcgcc ctccttcaac atgggccacc cggtcgcctt cgacttcgcc
    11761 gagcgcctcg cggccgtcgc gcccgggccg gacggcgcca agctcgaccg catcttcttc
    11821 accggctcgg gctcggagag cgtcgacacg gcgctgaaga tcgccatcgc ctaccagcgc
    11881 gccatcggcc agggcacccg cacccgggtc atcggccgcg agcgcggcta tcacggcgtc
    11941 ggcttcggcg gcatctcggt cggcggcctc gtcaacaacc gccgggtctt cccgcagatt
    12001 ccggccgacc acctgcgcca tacccacgac ctcgcgcgga acgcctttgt caagggccag
    12061 cccgagcacg gcgccgagct cgccgacgac ctccagcgcc tggtcgacct gcacggcgcc
    12121 gagaccattg ccgcggtgat cgtcgagccc gtcgccggct cgaccggcgt cctcgtcccg
    12181 cccaagggct acctcgagcg gctgcgggag atcgcgacga agcacggcat cctcctgatc
    12241 ttcgacgagg tcatcaccgg cttcggccgc ctcggcgcgc ccttcgccac cgacttcttt
    12301 ggcgtcaccc cggacctcgt gacgacggcc aagggcctga ccaacggcat catcccgatg
    12361 ggcgccgtct tcgcctcgcg cgccgtccac gacgcgatga tgaccggccc ggagaacgcc
    12421 atcgagttct tccacggcta cacctactcc ggccatcctg tcgcctgcgc cgccggcctc
    12481 gccgcgctcg acatctacga gggcgacggg ctccttaccc acgcggccga gcttcaggcg
    12541 aagtgggacg cggcgatcca ctcgctgaag ggtgcaagca acgtcatcga catccgcacg
    12601 atcgggctga tcgccggcat cgagctcggc tcgcgcgagg gcgcgcccgg gacgcgggcc
    12661 tacgacgtct tcgtcgactg cttggaaaag ggcctgctga tccgcgtcac cggcgacatc
    12721 atagcgctct cgccgccgct gatcgtcgag gagggccaga tcgacgagat cgtgtcgacc
    12781 cttggcgacg cgctgaaccg cgccgcctga accggcctcc acgcaaggcg ggagatgacg
    12841 atgctcgacg ctgtccttcg gcgcgccggc gccgtgctcg agatcgatct cggggcgatc
    12901 cgggagaatt accggatcct ccgggccaag gcgcgtggcg gctgcgccgc cgtggtcaag
    12961 gccgacgcct atggcctcgg ggcgacggtc gtcgccccgg ccctccacgc cgaaggggcg
    13021 cgcagcttct tcgtcgcgca cctcgacgag gcgctggcgc tccagccggc tctccccgtc
    13081 gggacccaga tcctggtcct gaacggcctt ccccctggag ccgaagcgga ctgcgccgcg
    13141 gccggtatcg ttccggtgct caacagcctc ggccaggtcg acgcctgggc cgccttcgcc
    13201 cggctgaagg gccggacgct cccagcggcg attcaggtcg acagcggcat gtcgcggatg
    13261 gggctgccgg aacacgacct cgcgcgcccc ggcgccgcgc tctacggcat cgcgcccgtg
    13321 gcgggtgagg cgaacccgat gaggccggtc gtgcgccttc gcggtcggat cgtgcaggtc
    13381 cgcgacatcc cggctggcgc acacgtcgac tatggcgcga gctggtgcgc cgctcagccc
    13441 agccggatcg cgaccgtctc ggtcgggtac gccgacggct acctgcgcag cctcagtcat
    13501 cgcgccggcg cgcatgtcgg cgggaccgcg gtgccgctcg tcggcatcgt gtcgatggac
    13561 agcatcacgt tcgatgtcac cgatgcgccc gacgcccggg caggcggctt cgtcgacctc
    13621 atcggccccg agaacccggt cgacgccgcc gccgaagccg gcggcaccat cggctacgga
    13681 atcctcacct cgctcggcag ctactcgccc ttgatcctga aggagcatgg catcgacctg
    13741 ccggtctacc cggtgaaggg ctattcgctg accgtaccga tcaccgagcc gtcgggcgcg
    13801 cccgagtcaa cggtgatgga cgagaagcac aaggtggcga tcacccgcct cggcgaccgg
    13861 atccgggtcg gtggcatggc cgagctcgac ggctactcga ccgatctgca cccaaaccgc
    13921 cgggccacgc tcgagcacgt cgtctccgac ctctacccga cgggcggcga cgccaaggcg
    13981 ggcacgtttt gggcgggcca ccggccgatg acccccgacg gcccgcccat gatcggtcgg
    14041 acgaagtacc cgaacctctg gctcaacacc ggccacggca ccctcggctg gacgatggcc
    14101 tgcggctcgg gcgccgtact cgccgatctc gtctccggcc gcaccccggc aatcgacgcc
    14161 cgcgcactcg gcatcgagcg ctacgccgcg gccgccaagg ccctcagcaa cgactcgaag
    14221 gaagtccccg tatgaccccg atcaacgcct caggcgctcc cgccgcaatc ggtccatact
    14281 gccacgccgt aaaggttggc gacctgctct tcacctcggg ccagatcccg ctcgacctcg
    14341 cgggcgcgat gccggagggc gtcgaagcgc agaccgacca ggtcttcgac aatctcgccg
    14401 cggccctgaa ggaaaccggc gcctcgctcg accaggtagt gaaggcgacc gtcttcgtca
    14461 ccgacctcgg cgacttcgcg aagctcaacg cggtctacga gcggcgcttc ggcgcccaca
    14521 agccggcccg ctcgacggtg caggtcgcgc cctgccgcgc ggcgcctcgg tcgaaatcga
    14581 actcgtcgtg caactgcgcg acgcctgaac cgaagctgaa ccccgtcccg caagcgacgg
    14641 ccatggagga aagaggatga ctgacctaac ccattacgtt ggcggcgggc ccgtcgcggg
    14701 ccgttctggc cggttcgcgg atgtcttcaa cccggcgacc ggcgccg
//
LOCUS       DAOTFI010000119        14729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4898_length_14729_cov_7.151492, whole genome shotgun sequence.
ACCESSION   DAOTFI010000119 DAOTFI010000000
VERSION     DAOTFI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4898_length_14729_cov_7.151492"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..100)
                     /gene="ffs"
                     /locus_tag="P5558_13170"
     ncRNA           complement(8..100)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5558_13170"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            227..1144
                     /gene="nudC"
                     /locus_tag="P5558_13175"
     CDS             227..1144
                     /gene="nudC"
                     /locus_tag="P5558_13175"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRY25310.1"
                     /translation="MQPPRPANPPPHVYSSIGYDRAAARRLDSAWLRDALRAPDTRFL
                     PMHGLKPMFVEDETGPRACFVDAAALAGFRPDPPVLLGLVEGRPVFAVDLDGEEVAGG
                     TYLELRQATAGLPAVEAGLLAFARALAHWRLRNRFCGACGVEMTTTQAGHARHCGACG
                     FDTFPRTDPAVICLVTHGEHCLLGRSRHFPEGMFSTLAGFVEAGESLEQTVRREIEEE
                     VGLVVDDIHYRSSQPWPFPQSLMLGFRATAPLAPPVVDPAELEAARWVHRSELVDPDK
                     RPVRLPSPDSIARFLIDEWLYGEGSCGSG"
     gene            complement(1114..1632)
                     /locus_tag="P5558_13180"
     CDS             complement(1114..1632)
                     /locus_tag="P5558_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HRY25311.1"
                     /translation="MNRVAPLPDPAASGSEGSRPQVTADIGRGVIRLLADLGIAALPE
                     LPLANGRRVDVIGLGRDGGIHVVEIKSSRADFMTDCKWPDYLEFAERFYFAVAVDFPL
                     DLLPEDEGLILADRYGAELVRPARHRPLAAARRKAVTLRFARLAAQRLALPLLAPDAG
                     LPLQPEPQDPSP"
     gene            1727..2896
                     /locus_tag="P5558_13185"
     CDS             1727..2896
                     /locus_tag="P5558_13185"
                     /inference="COORDINATES: protein motif:HMM:NF021603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HRY25312.1"
                     /translation="MTQKLATAAPGEGNSRRRMAIAERGRAVAERLTGRDVVALQEDP
                     SAANRALIARKFGESYDELVESSPRDLVAALLGLMVGDVEAQVRRALAVAVADSDKLP
                     ARIVGQLARDRIEVARPILEQSPLLQDPELIEIVRTNAMQYALAVAGRERISESLADA
                     LVDSGQQPVVVRLVGNAGAQLSAKALGRVMADWAEDAEVQDRLVRRPALPFELVEQLV
                     GAIGDRIEWELVQTRKMPPEQARALMQAVRERTSIGLTAKEHGDRKLERHLRERLEAG
                     ALDHDAVLGLLRDGDVAAFELALGMHAKLEPARVRRLAYNDDRRHLAALCIRAGVPAP
                     HYVTIRMALDLAGRSVQPNQAKDDSYSSEAMQYLLHQYERLRLDEAAIEELIEEV"
     gene            complement(3051..3125)
                     /locus_tag="P5558_13190"
     tRNA            complement(3051..3125)
                     /locus_tag="P5558_13190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3091..3093),aa:Thr,seq:ggt)
     gene            3266..3484
                     /locus_tag="P5558_13195"
     CDS             3266..3484
                     /locus_tag="P5558_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25313.1"
                     /translation="MADEEERPAVTAAGAGRQKEREARAAAALRANLLRRKAQQRGRD
                     GAAAARSDDGSGDAVDDTSGGTGSRRTS"
     gene            3569..4123
                     /gene="dcd"
                     /locus_tag="P5558_13200"
     CDS             3569..4123
                     /gene="dcd"
                     /locus_tag="P5558_13200"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRY25314.1"
                     /translation="MSIMPDSWIRRMAESTGMIEPFLDRQERKGVISYGLSSYGYDAR
                     VAPEFKIFTNVDNAIVDPKDFSDASFVERNGPSCTIPPNSFALARTVEYFRIPRDVLV
                     ICLGKSTYARCGMIVNVTPLEPEWQGHVTLEISNTTPLPARVYANEGICQFLFLKGDG
                     PPEISYADRAGKYQGQRGVTLPRL"
     gene            4258..4743
                     /locus_tag="P5558_13205"
     CDS             4258..4743
                     /locus_tag="P5558_13205"
                     /inference="COORDINATES: protein motif:HMM:NF022612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HRY25315.1"
                     /translation="MIEVEEREGVRLRAEDVEDLAVMAALLQDGRVALREMAYDPEER
                     RFIAAFIRYRRELQDDPTSCAGLTEIGVALRFDCIAQVKHRNLDPDDLERQLTLLTIA
                     TEPGERQLFHIDLVFEGGPEIQLRTDCIDVLLEDFGKPRPCRVSPCDHFANEFGLGAK
                     A"
     gene            4831..5274
                     /locus_tag="P5558_13210"
     CDS             4831..5274
                     /locus_tag="P5558_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HRY25316.1"
                     /translation="MELDERSIARWSPEIEHERNVAIFDLLEENRFSLVEAACGPYRL
                     NLSLRDATLVLAVTNARGELIEIALSARPLRRVVKDYFLICESYFGAIRGASPGRIEA
                     IDMARRGLHNEGAELLREALGERITVDSNTARRLFTLVCVLHLRG"
     gene            5274..5720
                     /locus_tag="P5558_13215"
     CDS             5274..5720
                     /locus_tag="P5558_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HRY25317.1"
                     /translation="MSELPGAVLFACSHNQIRSPMAEALLKYLHGRRIFVQSAGVRAG
                     DAVDGFAVAVLDELGLDLKGHRPRSFDQLDDDYFDLVISLSPEAQHRAVELTRTSSCE
                     LEFWHMPDPSLVDGTREQRLDAYRELRSMLLERLRQRFPVAGGPGL"
     gene            5881..6465
                     /locus_tag="P5558_13220"
     CDS             5881..6465
                     /locus_tag="P5558_13220"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRY25318.1"
                     /translation="MSRLGLVLASSSPRRLELLRQVGIEPDRVMDPAINEGERPRELP
                     RQHALRLAVEKATAVADRLDGPAFVLAADTVVACGRRILPKVSDEATARRCLELLSGR
                     RHRVHGGVAVRTPDGRLVSRLVTTQVRFRRLAEAEILAYLASAEWQGKAGGYAIQGRA
                     ARFVPWINGSYANVVGLPLVETLALLEGLGWQAP"
     gene            6462..7679
                     /locus_tag="P5558_13225"
     CDS             6462..7679
                     /locus_tag="P5558_13225"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRY25319.1"
                     /translation="MTVELILDRLGDAVALAVVENDLLVDLWAQGGGPEAVEDRLFLA
                     RVIGLEPAVDGAFLDHGAGRPGFLAGKDARHRRGLAKGAAIGRTVEVGEWLVVQGMRA
                     AEGDKGARLTTDIRLAAAGLVYRPHGSDIQLGRGIAARDREAMALRGAGLLDKAKLPG
                     GLVLRRLAIEADDAALAQDLGRLGALWAKLDRLASTAERRSGPLDAGPSALARLLWRA
                     LDLAPVSLTVADDALKAEARRLVQALPEAVRPELELLDSGTSAFALTGVDEAIAEALS
                     GEVPLDGGGRLLIQETAACVAIDVDGGGRPALDVNLAAAAEIGRLARLRNLGGTLIVD
                     FVDLAGKAQRQRLDDAVRRAFRGDPLPVQTFPMSPLGIVQISRARRGASPLGAASRIC
                     PACGGSGRTAAGP"
     gene            7676..7897
                     /gene="yacG"
                     /locus_tag="P5558_13230"
     CDS             7676..7897
                     /gene="yacG"
                     /locus_tag="P5558_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HRY25320.1"
                     /translation="MSMAPPPDRPARERRQRRCPLCGAPMEPRYRPFCSARCRDIDLG
                     RWFSGAYAVPAVEGDDETDEGGEFGGRDG"
     gene            complement(7923..8522)
                     /locus_tag="P5558_13235"
     CDS             complement(7923..8522)
                     /locus_tag="P5558_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25321.1"
                     /translation="MASFGLAFWGTGPGAVGADADGCAPLTVPDELGLVCRTDRTGQG
                     EILVVEPEGGTFRALSRLTLRELDPEVDRLAWSEPEAWLERQMVVDVGALSAALDEVG
                     GDPDSPFGSEMVKNGIAMLVDGLEELSRLPLSACGGAATEEEIDCRFGIEPFLLFMRV
                     SLVARYEARFAFNIRTFNEQRLRHFTAIANSFEAKSPDP"
     gene            complement(8531..8797)
                     /locus_tag="P5558_13240"
     CDS             complement(8531..8797)
                     /locus_tag="P5558_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25322.1"
                     /translation="MSTFCFSVQSDAEPGVLPRVLEVFALHGFVPDQCHATRCGDSGD
                     ELVVDLQMRGLDHAAAALLAKRLGRVVAVSTVLFSEKWQQCAAA"
     gene            complement(8963..9448)
                     /locus_tag="P5558_13245"
     CDS             complement(8963..9448)
                     /locus_tag="P5558_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25323.1"
                     /translation="MGERTRVRINLAQAEIEVEGSESFVESFRPQLEALLDRLPREPA
                     APPAADQVLAIPPAARAKTELGPFGEFLHHLPASATEVDRMLAAGYYLQQLSGDDSFA
                     TGDANKRLAEHGIKIGNPSQCVRQSLTARRVFAVARGRFRVSQTGRAYLRQLTNGTVP
                     E"
     gene            complement(9529..9975)
                     /locus_tag="P5558_13250"
     CDS             complement(9529..9975)
                     /locus_tag="P5558_13250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRY25324.1"
                     /translation="MSSHDRPPPSNRAAFPFFATITTRWADNDVYGHANNVIYYSYFD
                     TAIAQLLVGQGGLDPDAGPVIGLAVENGCRFHSSVGFPDIVHAGVGVARLGTSSVRYR
                     IGIFRNDETTAAADGHFVHVFVDRLTQRPTPIPPGIRTVLEAHTMD"
     gene            complement(9981..11384)
                     /locus_tag="P5558_13255"
     CDS             complement(9981..11384)
                     /locus_tag="P5558_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRY25325.1"
                     /translation="MLACFAPRAGADATPVRLIAAADYVAWRDGTSEATRAWLEATGF
                     RPKRHRLALLPDGEGGIAEAIFLIDGPGGAADAAAMAATLPARERPWRIDDPEGLLAP
                     FDLHHGWALAGYRFDRYVSAEAGNGGNGTARLATNDTATAERASQLALSVFLARDLVN
                     TPANDLGPAELETAIREVAREFGATCTSIAGDELLAANYPMIHAVGRASSRPPRLVDL
                     IWGSLDAPKLTLVGKGVCFDSGGLDIKPAAGMRLMKKDMGGAAVMTGLARMIMAAGLD
                     VRLRLLVPAVENSIGASAFRPGDVLSTRKGLTVEVGNTDAEGRLILADALAEADREQP
                     ALLLDAATLTGAARVAVGTDLPALFTPDERLAAELLQAGIDVAEPLWRLPLHPGYRDL
                     LKSPVADLSSTGSKPYAGAITAALFLQRFVEPTTSWAHLDIFAWNEDARPGRTKGGDA
                     TALRALFTLIERRYQKS"
     gene            complement(11415..14084)
                     /locus_tag="P5558_13260"
     CDS             complement(11415..14084)
                     /locus_tag="P5558_13260"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRY25326.1"
                     /translation="MLDKSFDAAAAEPRIYAAWEETGAFAPRDSETAGGRDDGGKPFV
                     VMMPPPNVTGSLHIGHALDQTIQDALTRYHRMRGRPTLWQPGTDHAGIATQMMVERKL
                     AAEGRDRRSIGRDAFLAECWSWKEASHGNIIGQLKRLGASPDWSRERFTMDDTLSRAV
                     TRAFVQLFKEGLIYKDKRLVNWDVQLQTAVSDLETQPVEVDGHLWHLDYPLEGEDGRV
                     ITVATTRPETMLGDTGVAVHPEDERYRDLIGRHVVLPLVGRRIPIVADSYSDPEKGTG
                     AVKITPAHDFNDFEVGRRHGLETIAILDATGRINDTAPEAYRGLDRFEARKRVVADME
                     AQGRLATVEKHRHTVPHGDRSGTPLEPYLTDQWYCDAATLAKPAIEAVETGRTVFVPA
                     QWQSTYFEWMRNIQPWCISRQLWWGHRIPAWYGPDGHIIVAETEAEAEAAAERHYGQK
                     VELARDPDVLDTWFSSGLWPFSTLGWPEETPDLARFYPGTVLVTGFDIIFFWVARMMM
                     QGLHFMGEVPFEAVHIHGLVRDEKGQKMSKTKGNVVDPLGLVDSYGADALRLSLLAST
                     AHGRDIRFGASRVEGYRNFVTKLWNAARFAELNGAVLRTDFRPDSTTLTLNRWIVGAV
                     ARVTEATTAAVDAYRFNDATLGLYHFVWNDFCDWYIELAKPLLLGEDEAARAETQATL
                     GWALARILHLLHPFAPFVTEALWRDLFGAPGGMLIGAEWPGGETAGSVDPAAGAEIDW
                     LIRLVGAIRAARNEVNVPAGAKLQLRVQGAGPQTLERLATHGEALRRLARLAAVEPQA
                     SGAAAKSLQLVVDEATYVLPVGEVIDLEVERQRLAREVEKLQGEAKKLEGKLANEAFL
                     ARAPAEIVEEQRERLAETEATSARLRAALARIA"
     gene            complement(14313..>14729)
                     /locus_tag="P5558_13265"
     CDS             complement(14313..>14729)
                     /locus_tag="P5558_13265"
                     /inference="COORDINATES: protein motif:HMM:NF022155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HRY25327.1"
                     /translation="VLDLTQVVDAGEAKPATPPALGADASELDLELEEDDDDEPTPAA
                     APPKPPAAAPRRIEEGLVSAPTASASTNAFARLAKAAASSEQPSRGFGDKTVEAFLTD
                     LLKPMLKDWLDQNLEGVVERVVEQEVKKLARRAELM"
BASE COUNT         2192 a         4921 c         5362 g         2254 t
ORIGIN      
        1 ggtcggtgga agactggcgc gcgacccgat gcgaagctcg ttacggctgc ttccttccgg
       61 acctgaccgg gttggcgagt gcatcgtccg cgaccagcct tccgccttag atatcgagcc
      121 tgctgggcgg ttgcgcaagg cccgtcgatg gcgcacaagc acgcattgcc caaaagcgag
      181 ggaccccgtc cgggggaccc gaggccagga aacccaggac cgaggcatgc agccgccgcg
      241 accggccaac ccgccacccc acgtctattc gtcgatcggc tatgaccgcg cggccgcaag
      301 gcgcctcgat tcggcttggc tgcgcgacgc cctcagggcc cccgacacac ggtttctccc
      361 catgcatggg ctgaagccca tgttcgtcga ggacgaaacg ggaccgcgtg cctgcttcgt
      421 cgatgccgcg gcccttgccg ggttccgccc cgatccgccg gtcctgctcg gcctcgtcga
      481 ggggcggccg gtcttcgccg tcgacctcga tggcgaggag gtcgccggcg gcacctacct
      541 ggagctgaga caggcgacag cgggcctgcc ggcggtcgag gcggggcttc tcgcctttgc
      601 gcgggcactg gcgcactggc gcctgcgcaa tcgtttctgc ggcgcctgcg gggtcgagat
      661 gacgaccacc caggcgggcc atgcgcggca ctgtggcgcc tgcggcttcg ataccttccc
      721 gcgcaccgat ccggccgtga tctgcctcgt cactcatggc gagcactgcc tgctcggccg
      781 ctcccgccat ttcccggaag gcatgttctc gacgctcgcc ggcttcgtcg aggcgggcga
      841 gagcctcgag cagacggtgc gccgcgagat cgaggaggag gtcggcctcg tggtcgacga
      901 cattcactat cgcagctcgc aaccgtggcc gttcccgcaa tccctgatgc tgggctttcg
      961 cgccaccgca cccctggcgc cgccggtggt cgatcccgcc gaactcgagg cggcgcgctg
     1021 ggtccatcgc agcgagctgg tcgatccgga caagcggccg gtccggctgc ccagtcccga
     1081 tagcatcgcc cgcttcctca tcgacgaatg gctctacggc gaggggtcct gcggctcggg
     1141 ctgaagcggc aggccggcat ccggggcgag caggggcagg gcgaggcgct gcgccgcgag
     1201 gcgggcgaag cgcagcgtga cggctttgcg ccgggcggcc gccagggggc gatggcgggc
     1261 cgggcggacg agctcggcgc cgtagcggtc ggcgaggatc aggccctcgt cctcgggcag
     1321 caggtcgagc gggaagtcga ccgccaccgc aaagtagaac cgctcggcga actccagata
     1381 atccggccac ttgcagtcgg tcatgaagtc ggcgcgcgaa ctcttgatct cgaccacgtg
     1441 aatgccgccg tcacggccga gcccgatgac gtcgaccctt cgcccgttgg cgagcggcag
     1501 ctcgggcagg gcagcaatgc cgagatcggc cagcaggcga atgacgcccc gaccgatatc
     1561 agcggtcacc tgtggccgcg agccctcgct gccactggcg gccgggtcgg ggaggggtgc
     1621 tacacggttc attaacccgg gatagctagg atgggcggat cggccaagct gtcgaacggg
     1681 gcgatatgga accgtcggcg gcgaagacag tagcgtggcg gaagacatga cgcagaaact
     1741 ggccacggct gcgccgggcg agggtaacag caggcgccgg atggcgatcg ccgaacgcgg
     1801 tcgggcggtg gcggaacgcc tgaccggtcg cgacgtcgtg gcgctgcagg aggacccgtc
     1861 ggccgccaac cgcgcgctga tcgcccggaa gttcggcgag agctacgacg agctggtgga
     1921 gtccagtcca cgcgacctcg tggcggcgct gctcggcctg atggtcggcg atgtcgaggc
     1981 gcaggtgcgg cgggcactcg ccgtcgcggt cgccgacagc gacaagctgc cggcccggat
     2041 cgtcggccag ctcgctcgtg accggatcga ggtggcccgg ccgatcctcg agcagagccc
     2101 ccttctccag gaccccgagc tcatcgagat cgtccgcacc aatgccatgc aatacgcgct
     2161 tgccgtcgcc gggcgcgaaa ggatttccga gtctctcgcg gatgcgctgg tcgattccgg
     2221 ccagcagccg gtcgtcgtgc gcctggtcgg caatgccggt gcccagctct cggccaaggc
     2281 gctcggccgg gtgatggccg actgggccga ggatgcggag gtgcaggacc ggctggttcg
     2341 gcggccggcc ttgcccttcg agctggtcga gcagctggtc ggtgccatcg gcgaccggat
     2401 cgagtgggag ctggtccaga cgaggaaaat gccacccgag caggcgcggg cgctgatgca
     2461 ggcggtgcgc gagcgcacat cgatcggcct gaccgccaag gagcatggcg accgcaagct
     2521 cgagcgccat ctgcgcgagc ggctggaggc cggggcactc gaccatgacg cggtgctcgg
     2581 gctcctgcgc gacggcgatg tcgcggcctt cgagctggcg ctcggcatgc atgcgaagct
     2641 cgagccggcc cgggtgcgcc ggctcgccta caacgacgac cgccgccatc tcgcggccct
     2701 ctgcatccgt gcgggggtgc cggcgccgca ctacgttacc atccgcatgg cgctcgatct
     2761 cgctggtcgc agcgtccagc cgaaccaagc caaggacgac agctacagca gcgaagcgat
     2821 gcaatacctg ctgcatcagt acgagcgcct caggctcgac gaggcggcga tcgaggagtt
     2881 gatcgaagag gtctaggagc ggcctgtgcg tgacatgagc ggcttgccgg gcatgggtcg
     2941 ccgggtccgg tcgtacgggc cgggccttgg cggccaaggg gcggagatgt aaaggccaaa
     3001 gccgggggct gatggcaacg ggcgaaggaa ttgttcggcg aaaaaaggaa tggtgctgct
     3061 ggtcaggatt gaactgacga cctctccctt accaagggag tgctctacca ctgagctaca
     3121 gcagcatccg ggcgcacgac cccatccatc agtctttggc ggctgggtcg gccggcggcg
     3181 gtgtggtaga tgccacacgg gaggcgggtg tgcaagcgag gcggtggtgc ccgcaacggc
     3241 cgattccgga tcgggagacg atgcgatggc ggacgaggag gagcggccgg cggtgaccgc
     3301 tgccggagcc gggcggcaga aggagcggga ggcaagggcg gcggcggcgc tgcgggccaa
     3361 tctcctcagg cgcaaggcgc agcagcgcgg acgggatggt gccgctgccg cacgatccga
     3421 cgatggctca ggcgatgcgg tggacgatac gtccggcggg accggatcca ggaggacgtc
     3481 gtaagtcagg ttgagcgcgg cggcttcggc ctctaagaac gggcgacgcg ccgggccggc
     3541 gctggagtct tttggagacc gaccgccgat gtcgatcatg cccgacagct ggattcgccg
     3601 catggccgag agcaccggca tgatcgagcc gttcctcgac cggcaggagc gcaagggcgt
     3661 catcagctac ggactctcga gctacggcta cgacgcccgg gtggcgccgg aattcaagat
     3721 cttcaccaac gtcgacaacg ccatcgtcga tccgaaggac ttctcggatg cgagcttcgt
     3781 cgagcgcaac ggaccatcct gcacgatccc acccaacagc ttcgcgctcg cccgcacggt
     3841 cgagtatttc cgcattccgc gcgatgtcct ggtgatctgc ctcggcaagt cgacctatgc
     3901 gcgctgcggc atgatcgtca acgtgactcc gctcgagccg gaatggcagg ggcacgtgac
     3961 cctcgagatc tccaatacga caccgctgcc cgcccgggtc tacgccaacg agggcatctg
     4021 ccagttcctc ttcctcaagg gcgatggccc gccggagatc tcctatgccg accgcgccgg
     4081 caagtatcag ggccagcgcg gcgtaacctt gccgcgactc tgaccggaga gggttgccgg
     4141 cgcgggccgg ctcacccggc gctctacagg gctttgcact agcgcattcg atcccacatg
     4201 aaggagcggt ccgggcgcag ggccacctgg aatgcacaag ctcgagggca tgcgaggatg
     4261 atcgaggtcg aggaacggga aggcgtgcgc ctgcgggcgg aggacgtcga ggatctggcc
     4321 gtcatggccg cactcctgca ggacgggcgc gtggcgctgc gcgagatggc ttacgacccg
     4381 gaggagcggc ggttcatcgc cgccttcatc cgctaccggc gcgagctgca ggacgatccg
     4441 accagttgcg ccgggctcac cgagatcggc gtggcgttgc gcttcgattg catcgcccag
     4501 gtcaagcacc gcaatctcga tccggatgac ctcgagcgcc agctcaccct cctgaccatt
     4561 gcgacggagc cgggcgagcg gcagctcttc cacatcgatc tcgtcttcga gggcggaccc
     4621 gagatccagc tcaggactga ctgcatcgat gtgctgctgg aggacttcgg gaagccacgt
     4681 ccttgccggg tcagtccctg cgatcacttc gccaacgagt tcgggcttgg cgccaaggcc
     4741 tgagcggacg cgcggctggt gagcgggtca aaggtctccg gttccgcggc cagcggggac
     4801 gcggctcccg aggccgaacg ccttgccgcg atcgagctcg acgagcggag catcgcccgc
     4861 tggagccccg agatcgagca tgagcgcaat gtcgcgatct tcgacctgct tgaggagaat
     4921 cgcttctcgc tggtcgaggc ggcgtgcggc ccctatcggc tcaacctatc gctgcgtgac
     4981 gcgacattgg tgctggcggt caccaacgcg cggggtgagt tgatcgagat cgcactctcg
     5041 gcccgtcccc tgcggcgcgt ggtcaaggac tacttcctga tctgcgagag ctatttcggc
     5101 gccattcgcg gggcctcgcc cggccgcatc gaggcgatcg acatggcccg gcgcggcctg
     5161 cacaacgaag gtgcggagct gctgcgtgag gcgctcggcg agcggatcac ggttgacagc
     5221 aacaccgcac gccgtctctt cacgctggtc tgtgtcctgc atctgcgcgg ctgatgagcg
     5281 agctaccggg tgccgtcctc tttgcgtgct cgcacaacca gattcgctcg ccgatggcgg
     5341 aagcgctgct caagtacctg cacgggcgcc ggatcttcgt ccagagtgct ggggtgcggg
     5401 caggcgacgc ggtcgacggc ttcgccgtcg cggtgctcga cgagctcggg ctcgacctca
     5461 agggccaccg gccgcgcagc ttcgaccagc tcgacgacga ctacttcgat ctcgtgatct
     5521 cgctctcgcc tgaggcgcag cacagggcgg tggagctgac gcgcaccagc tcctgcgagc
     5581 tcgagttctg gcacatgcct gacccgagcc tcgtcgacgg cacgcgcgag cagcggctcg
     5641 atgcctatcg cgaattgcgc tcgatgttgc tcgaacggct gcgccagcgg tttccggtgg
     5701 cgggcgggcc ggggctctga gccgggaccg gccgtccttg accacagcgc tccctgcgcc
     5761 catgaacggc gccggccacc gaccgattgg caccccgacg accttccctt cccgttcctt
     5821 cagcaggagt ccgatgcaca tcccgctggc ccagcccaca tcgcagacga gtactgccgc
     5881 ttgagcaggc tcgggctggt gctcgcctcg tcgtcaccgc gtcgcctcga gctcctgcgc
     5941 caggtcggca tcgagccaga ccgggtgatg gatcccgcga tcaacgaggg tgagcgcccg
     6001 cgcgagctac cgcgccagca tgcccttcgc cttgccgtcg agaaggccac ggccgtggcc
     6061 gaccggctcg acggtcccgc cttcgtcctc gctgccgaca ccgtggtcgc ctgcggccgg
     6121 cgcattctgc cgaaggtctc ggacgaggcg accgcccgac gctgcctcga gctcttaagc
     6181 ggccgccgcc accgggtgca tggcggggtc gccgtccgca cccccgacgg gcggctggtc
     6241 agccgtctcg tgacgaccca ggtacgcttc aggcggctgg cggaggccga gatcctggcc
     6301 tatctcgcct cggccgagtg gcaaggcaaa gccggcggct atgcgatcca gggccgcgcc
     6361 gcgcggttcg tgccgtggat caatggctcc tatgcgaacg tggtgggcct gccgctggtc
     6421 gagacgctgg ctctcctcga ggggctcggg tggcaggcgc catgacggtg gagctgatcc
     6481 tcgaccggct gggggacgcc gtggcgctgg cagtggtcga gaacgacctg ctcgtcgacc
     6541 tctgggccca gggcggcggg ccggaggcgg tcgaggaccg gctcttcctc gcccgcgtga
     6601 tcggcctcga gccggctgtc gacggcgctt tcctcgacca tggcgccggc cggcccggtt
     6661 tcctcgcggg caaggatgcg cgccatcgcc gggggctcgc caagggcgct gcgatcggcc
     6721 gcacggtcga agtcggcgaa tggctggtcg tccagggcat gcgggccgcc gagggcgaca
     6781 agggagcacg gctcaccacc gacatccgcc tcgccgcggc ggggctcgtt taccggccgc
     6841 atggcagcga catccagctc ggccggggca ttgccgcccg cgaccgcgag gccatggcgc
     6901 tcaggggtgc cgggctgctc gacaaggcga agctgccggg cgggctggtg ctccgccggc
     6961 tcgccatcga ggcggacgat gcggcgcttg cccaggatct cggccggctc ggcgccctct
     7021 gggcgaagct agaccggctt gccagcacgg cggagcgacg gtcgggcccg ctcgatgccg
     7081 gcccgagcgc gctcgcccgg ctcctctggc gggcgctcga cctcgcaccc gtctcgctta
     7141 cagtcgccga tgacgccctg aaggctgaag cgcgacggct ggtgcaggcc ctgcccgagg
     7201 ccgtccggcc cgagctcgag ctgctcgatt caggcacctc cgcctttgcc ctgaccggcg
     7261 tcgacgaggc gatcgccgag gcgctttcgg gcgaggtgcc gctcgatggc ggcgggcgcc
     7321 tcctgatcca ggagacggcg gcctgcgtcg ccatcgatgt cgacggcggc gggcggccgg
     7381 cgctcgacgt caatcttgcg gcggcggcgg aaatcggtcg gctggcgcgg ctgcgcaatc
     7441 tcggtggcac gctgatcgtc gatttcgtcg atctcgccgg caaggcgcag cgccaacggc
     7501 tcgacgacgc ggtacgccgg gccttccgcg gcgatccttt gccggtccag accttcccca
     7561 tgtcgccgct gggcatcgtc cagatcagcc gtgcccgtcg cggtgccagc ccgctcgggg
     7621 cggcgagccg gatctgtccg gcctgtggcg gcagtggccg cacggcggcg ggcccatgag
     7681 tatggcacct ccgcccgacc ggccggcgcg cgagcggcgc cagcgccgct gcccgctctg
     7741 cggtgcgccg atggagccgc gctatcgccc cttctgctcg gcccgctgcc gcgacatcga
     7801 tctcggccgg tggttctccg gcgcctatgc ggtgccggcg gtcgaaggcg acgacgagac
     7861 ggacgagggc ggggagttcg gcggccgcga cggttgagcg ccccgcggag ggcggaacgt
     7921 gctcagggat ccgggctctt cgcctcgaac gagttggcga tcgcggtgaa gtggcggagc
     7981 cgctgttcgt tgaacgtgcg gatgttgaag gcaaagcgcg cctcgtaccg ggcgacgagc
     8041 gagacgcgca tgaaaaggag gaagggctcg atgccgaagc ggcagtcgat ctcctcttcg
     8101 gtcgccgccc cgccacaggc ggagagcggc agccggctca gctcctcgag gccgtcgacc
     8161 agcatggcga tgccgttctt gaccatctcg ctgccgaagg ggctgtcggg atcgccgccg
     8221 acctcgtcga gggcagccga gagggcgccc acatcgacga ccatctgccg ctcgagccaa
     8281 gcctcgggct cactccaggc gagcctgtcg acctcgggat cgagctcgcg gagcgtcagc
     8341 cggctgagcg cccggaaggt tccgccttcg ggctcgacca ccaggatctc gccctggccg
     8401 gtgcggtcgg tgcggcagac gaggccgagc tcgtcgggga cggtcagcgg cgcgcacccg
     8461 tccgcgtcgg caccgacggc gcccggcccg gtgccccaga aggcaagacc gaaggacgcc
     8521 aacagtgcgg tcaggcggcg gcgcactgct gccatttctc ggagaacagc accgtgctca
     8581 cggcgaccac gcgaccgagc cgcttggcca ggagcgctgc agcggcgtgg tcgagacccc
     8641 gcatctgcag gtcgaccacg agttcatcgc cgctatcgcc gcaacgtgtg gcatggcact
     8701 ggtcggggac gaagccgtga agcgcgaaga cctcgagcac gcggggcagc acgccgggct
     8761 cggcgtccga ctggacggag aaacagaacg tggacatgac ggcgactttc gacaggtgat
     8821 ggcacgaaat aggtcgaaga cccggcctca gctcgagacc gggcgcctaa ttcgaatcac
     8881 ctggcggatg ctcatcatga acttcggtta gcgccggccg gcggacgcgt caagtcgggc
     8941 gcggcgccaa aagtgtccgc gctcactcgg gtactgtacc attggtgagc tgccggagat
     9001 aggcgcggcc agtctgcgac acccgaaagc ggccgcgggc cacggcgaag acgcgccttg
     9061 ccgtaagcga ctggcgcacg cattgtgacg ggttgccgat cttgatgccg tgctcggcga
     9121 gccgcttgtt ggcatcgccc gtggcgaagc tgtcgtcgcc cgagagttgc tgcaggtagt
     9181 agccggccgc cagcatgcga tcgacctctg tggcgcttgc cggaagatgg tggaggaact
     9241 cgccgaacgg gccgagctcg gtcttcgccc tggcggcggg cgggatggcc agcacttggt
     9301 cggccgcggg cggcgctgcc ggctccctgg gcagccggtc gagcagggcc tcgagttgcg
     9361 ggcggaagct ctcgacgaag ctctcgctgc cctcgacctc gatctccgcc tgagccagat
     9421 tgatccgcac ccgcgtccgc tcgcccaagg ccggcatcct ctttggaact tccagccttc
     9481 ctacgccatt aaccgtgccc ccgcaagttg agcgaggctc agtcggtatc agtccatggt
     9541 gtgggcctcg agcaccgtgc gaatgccggg cgggatgggc gtcggtcgct gggtcaggcg
     9601 gtcgacgaag acatggacga aatgcccgtc ggcggcagcg gtggtctcgt cgttcctgaa
     9661 gatgccgatc cggtagcgga ccgaggaggt accgagccgc gccacaccca cgccggcatg
     9721 gacgatgtcc ggaaagccga ccgagctgtg gaaccggcaa ccattctcga cggcgaggcc
     9781 gatgaccggg ccggcatccg gatccagccc tccctggccg accaggagct gggcgatcgc
     9841 cgtgtcgaaa tagctgtaat agatcacgtt gttggcgtgg ccgtagacat cgttgtcggc
     9901 ccaacgggtg gtgatggtgg caaagaaggg aaaggcggcg cggttcgagg gcggcggccg
     9961 gtcgtggctc gacatggggt tcagctcttc tggtaacgac gctcgatgag ggtgaagagg
    10021 gcgcggagtg ccgtggcgtc gccgcccttg gtccggcccg ggcgggcatc ctcgttccag
    10081 gcgaagatgt cgagatgcgc ccaggatgtg gtcggctcga cgaaacgctg gaggaagagg
    10141 gcggcggtga tcgccccggc atagggcttg ctgccggtgc tcgagagatc ggcgacgggg
    10201 ctcttcaaga gatcgcggta gcccgggtgc agcggcaggc gccagagcgg ctcggccacg
    10261 tcgatgccgg cctgaagcag ctcggcggcg aggcgctcgt cgggggtgaa gagggctggc
    10321 agatccgtgc cgacggcgac gcgcgcggcc ccggtcagtg ttgccgcatc gagcaggaga
    10381 gccggctgct cgcggtccgc ctcggcgagc gcgtcggcga ggatgagccg cccctcggcg
    10441 tcggtgttgc cgacctcgac cgtgagcccc ttcctggtcg agagcacgtc gcccgggcgg
    10501 aacgcggagg cgccgatgct gttctcgacc gccggaacga gcaggcgcag gcgcacgtcg
    10561 aggccggccg ccatgatcat gcgggcgagg ccggtcatca ccgcggcacc gcccatgtcc
    10621 ttcttcatca gccgcatgcc cgcggcgggt ttgatgtcga gcccgcccga atcgaagcag
    10681 actcccttgc cgacgagcgt gagctttggc gcgtccaacg aaccccagat gagatcgacg
    10741 agccggggcg ggcggctgct ggcccggccg acggcatgga tcatgggata gttggcggcg
    10801 agcagttcat cgcccgcgat gctggtgcag gtggcgccga attcccgggc gacctcccgg
    10861 atcgccgtct cgagctcggc ggggccgaga tcgttggccg gggtgttgac gaggtcgcgg
    10921 gcgagaaaga cggagagtgc gagctggctg gcccgttccg cggtcgccgt atcgttcgtg
    10981 gcgaggcgag cggtgccatt gccgccgttc ccggcctccg cgctgacgta gcggtcgaaa
    11041 cggtagccgg cgagcgccca gccatggtgc aggtcgaagg gggcgagcag gccctcggga
    11101 tcgtcgatgc gccaaggccg ctcgcgtgcc ggcagggtcg ccgccatggc cgcggcatcg
    11161 gcggcaccgc ccggcccgtc gatgaggaag atcgcttcgg caatgccgcc ctcgccatcg
    11221 ggcaggagcg cgagacgatg gcgtttcggg cgaaagcccg tggcctctag ccaggctcgg
    11281 gtggcctccg aggtaccgtc ccgccaggcg acgtagtcgg cggcggcgat gagccggacg
    11341 ggcgtggcgt cggcgccggc tctgggggcg aagcaggcga gcatggtggg tctccggctg
    11401 gctgggctgg acgctcaggc gatgcgggcg agggcggccc tgagcctggc gctcgtggcc
    11461 tcggtttcgg ccaggcgctc gcgctgctcc tcgacgattt ccgcgggtgc ccgggcgagg
    11521 aaggcctcgt tggcgagctt gccctcgagc tttttcgctt cgccctggag cttctccacc
    11581 tcgcgggcga gccgctggcg ctcgacctcg agatcgatca cctcgccgac cggcagcaca
    11641 taggttgcct cgtcgacgac gagctgcagg ctcttcgcgg cggcaccgct ggcctgcggc
    11701 tcgaccgcgg cgagccgggc gaggcggcgc aacgcctcgc catgggtggc gagccgctcc
    11761 agcgtctggg gcccggcacc ctggaccctg agctgcagct tcgcacccgc cggcacgttg
    11821 acctcgttgc gcgcggcgcg gatggcaccg accagccgaa tcagccagtc gatctccgcc
    11881 ccggcggccg gatcgaccga ccccgcagtc tcgccgccgg gccactcggc gccgatcagc
    11941 atgccgccgg gtgcaccgaa gaggtcccgc cagagcgctt cggtgacgaa cggggcgaag
    12001 ggatggagga gatggaggat gcgcgccagg gcccagccga gcgtcgcctg cgtctcggcc
    12061 ctggccgcct cgtcctcgcc gagcagcagg ggcttcgcca gctcgatgta ccagtcgcag
    12121 aaatcgttcc agacgaagtg gtagaggccg agcgtcgcgt cgttgaagcg gtaggcgtcg
    12181 accgccgcgg tggtggcctc ggtcaccctg gcgacggcgc ccacgatcca gcggttgagc
    12241 gtcagcgtcg tcgagtcggg acggaaatcg gtccgcagga ccgcaccgtt gagctcggcg
    12301 aagcgggcgg cgttccagag cttggtgacg aaattgcggt agccctcgac gcgcgacgcc
    12361 ccgaagcgga tgtcgcggcc gtgcgccgtg ctggcgagca gcgagaggcg cagcgcatcg
    12421 gcgccatagc tgtcgacgag gcccagggga tcgaccacgt tgcccttggt cttcgacatc
    12481 ttctggccct tctcgtcacg gacgaggcca tggatgtgga ccgcctcgaa gggcacctcg
    12541 cccatgaagt gcaggccctg catcatcatc cgggcgaccc agaagaagat gatgtcgaag
    12601 ccggtgacga gcaccgtgcc cgggtagaaa cgggcgagat cgggcgtctc ctccggccag
    12661 ccgagggtcg agaagggcca gaggccggac gagaaccagg tgtcgagcac gtccggatca
    12721 cgggcgagct cgaccttttg gccataatgc cgttccgccg ccgcctcggc ctcggcctcg
    12781 gtctccgcca cgatgatgtg gccgtccgga ccgtaccagg cggggatccg gtggccccac
    12841 cagagctggc ggctgatgca ccagggctgg atgttgcgca tccactcgaa atacgtgctc
    12901 tgccactggg cggggacgaa gaccgtccgg ccggtctcga ccgcctcgat ggcaggcttc
    12961 gccagggtgg cggcgtcgca gtaccactgg tcggtcaagt agggctcgag cggtgtgccc
    13021 gagcgatcac cgtgcggcac ggtatggcgg tgcttctcga ctgtcgccag ccggccctgc
    13081 gcctccatat cggcgacgac gcgcttgcgc gcttcgaagc ggtcgaggcc gcgataggct
    13141 tcgggggccg tgtcgttgat ccggccggtg gcatccagga tggcgatcgt ctcgagcccg
    13201 tggcggcggc cgacctcgaa atcgttgaag tcgtgcgccg gcgtgatctt cacggcgccc
    13261 gtgcccttct cggggtccga gtagctatcg gcgacgatcg ggatgcgacg gccgaccagg
    13321 gggaggacga cgtgccggcc gatcaggtcc cggtagcgct cgtcctccgg gtggacggcg
    13381 acgccggtgt cgccgagcat ggtctcgggc cgcgtggtcg cgacggtgat gacgcggccg
    13441 tcctcgccct ccagcgggta gtccagatgc cagagatggc cgtccacctc gaccggctgg
    13501 gtctcgaggt cggagacggc ggtctgcagc tggacgtccc agttgaccag ccgcttgtcc
    13561 ttgtagatca gcccttcctt gaagagttgg acgaaggcgc gggtgacggc tcgcgacaac
    13621 gtatcgtcca tggtgaaccg ctcgcggctc cagtcgggcg aggcgccgag ccgcttgagc
    13681 tggccgatga tgttgccatg gctcgcctcc ttccacgacc agcactcggc gaggaaggca
    13741 tcccggccga tgctgcgccg atccctgcct tccgccgcga gcttgcgctc gaccatcatc
    13801 tgggtggcga tgcccgcatg gtcggtgccc ggctgccaga gcgtcggccg gccgcgcatt
    13861 cggtggtagc gggtcagcgc atcctggatg gtctggtcga gtgcatggcc gatatgcagg
    13921 ctgccggtga cgttgggcgg cggcatcatc acgacaaagg gcttgccgcc gtcgtccctg
    13981 ccaccggccg tctcggagtc acgtggcgcg aaggcgccgg tctcttccca ggcggcgtag
    14041 atgcgcggct cggcagcggc cgcgtcgaag ctcttgtcca gcatggctgg agcccacctt
    14101 tcccgtctcg gccccgattg cggccacggc cggcccgaag ggccggcggg gcgcacgaat
    14161 ggtgcgccgc cagggccgat ggaggtgggg tttagtggaa ctcgtctggc gcgacaaggc
    14221 ggcagggtgg cgcgcccggg taaacgagct gcccgagcgc cgcgggtcgc cggtctcgct
    14281 cccgcccgcc cggcgccggg ccgcagcggg gctcacatga gctcggcgcg ccgggccagc
    14341 ttcttgactt cctgctccac cacgcgctcg acgacgcctt cgagattctg gtccagccag
    14401 tccttgagca tgggcttcag gaggtcggtg aggaaggctt caacggtctt gtcgccaaag
    14461 ccgcgggatg gctgctcgct gctcgccgct gccttggcga ggcgggcgaa ggcgttcgtg
    14521 ctggccgagg cggtgggcgc cgagacgagg ccttcctcga tgcgtcgcgg ggccgcggcg
    14581 ggaggcttcg gcggtgcggc ggcgggcgtg ggctcgtcgt cgtcgtcctc ctcgagctcg
    14641 aggtcgagct cgctggcgtc ggccccgagc gccggcggcg tggcgggctt cgcctcgcca
    14701 gcgtcgacga cctgggtgag atccaggac
//
LOCUS       DAOTFI010000120        14568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4967_length_14568_cov_7.495900, whole genome shotgun sequence.
ACCESSION   DAOTFI010000120 DAOTFI010000000
VERSION     DAOTFI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14568
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4967_length_14568_cov_7.495900"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="P5558_13270"
     CDS             <1..1202
                     /locus_tag="P5558_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25328.1"
                     /translation="AAELSIVSGAVGRDIDELRKHLDWFEERTGHTVSIVTMPASTSD
                     QFGQYRLWLSAQNPDIDVYRTDVIWAPQLASHFVDLTEHVQDVVGEHFPAIIESQTVD
                     GKIVALPMFTDAPALYYRTDLLEKHGFEPPTTWVGMAETAQAVMEAERAEGNAEIWGF
                     VWQGSAYEGLTCNALEWVKSNGGGQIVEPDGEISINNENAAEAIDLARTWVDTISPPG
                     VLGYKEEEARGVWQVGNSVFMRNWPYAYALSNGDDSAVKDKFAVVPLPIGPSGDRSAA
                     TLGGWNLAVSAYSPDQEAAIELVKFLASAEVQKDRAIETTRLPTIPALYEDAEVAEAQ
                     PVIPLWLDIFLNAVPRPSAPTKANYNQVSQEFWTAVHNTLSGTGDAATNLAELERNLK
                     RIRRSGW"
     gene            1317..2255
                     /locus_tag="P5558_13275"
     CDS             1317..2255
                     /locus_tag="P5558_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25329.1"
                     /translation="MAAATSQLTREKIRAAWIFLVPMMLVLLAVAGWPLLRTIWLGFT
                     DAKLSQPGSGQWIGFENYLFWLDYGEGDGEWIGLLADPTWWRAVWNTVYFTVIAVTIE
                     TLLGLIVALVLNAEFAGRGFVRAAILVPWAIPTIVSAKMWDWMMNDQFGILNDMFLKL
                     GLISAPIAWTAQPETAMIAVIIVDVWKTTPFMALLILAGMQMLPGDIYEAAKVDGIHP
                     VKVFFRVTLPLIRPAIMVAVIFRMLDSLRIFDLIYVLTPNNAQTVTMSVYARQHLFDF
                     DNFAYGSAASTLLFLIIAGITILYIYAGRLNLSGDK"
     gene            2255..3085
                     /locus_tag="P5558_13280"
     CDS             2255..3085
                     /locus_tag="P5558_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25330.1"
                     /translation="MRNRLLKQVGLWVLIVIISVYALFPFYYAIITSFKSGSAIFSVT
                     YLPDSFSLANYVGVLGSGHFPRNIVNSVIVATAVVILSLLLAITASYALARVRFKGRG
                     ILLFTILSVSMFPQIAVLAGLFEMVRGLGLYNTLFSLILAYMIFTLPFTVWVLTTFMR
                     DLPVELEEAAIVDGAKPHQIIFKVFMPLMWPALVTTGLLAFISAWNEFLFALTFVSNN
                     EQRTVPVAIALLSGATEQEIPWGPIMAASVIVTVPLVILVLVFQRRIVAGLTAGAVKG
                     "
     gene            3136..4233
                     /gene="ugpC"
                     /locus_tag="P5558_13285"
     CDS             3136..4233
                     /gene="ugpC"
                     /locus_tag="P5558_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY25331.1"
                     /translation="MATIKLDSVKKSFGKVDVIKGVDLDIKSQEFVVFVGPSGCGKST
                     LLRLVAGLEDITSGTLAIDGRVVNTLSPSDRGIAMVFQSYALYPHMTVYENMAFGLKL
                     AKTDPRSIEERVNEAARILQLDPLLQRLPKQLSGGQRQRVAIGRAIVRKPKVFLFDEP
                     LSNLDAALRVQMRIEIARLHHSLDATMIYVTHDQVEAMTLADRIVVLRDGYVMQFGTP
                     LDLYHHPENRFVGGFIGSPKMNFLPCDLVGDTAGQAVVETVAGTRVSTTSAINGQRPG
                     SGFTLGVRPEDLLAGGGGDAEIEGEVITVEHLGAETYIYVDLGMEEPLVVKAAGNSAV
                     SHGDRARVGIPAAACYLFDPKERALERPAYN"
     gene            complement(4264..5361)
                     /gene="ychF"
                     /locus_tag="P5558_13290"
     CDS             complement(4264..5361)
                     /gene="ychF"
                     /locus_tag="P5558_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRY25332.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTQTAAAAAANYPFCTIEPNVGR
                     LPVPDPRLDLLAKLGKSAKTLPTQLEIKDIAGLVRGASKGEGLGNKFLGAIREVDAIL
                     EVLRCFEDDDITHVEGGVDPLRDAELIQTELLLADIDSLNRQKDGLVKRIRAQDKEAK
                     RRLELIEHILPLMEEGRPVRRLPLDPEQRLLVADLTLLTAKPLLYVLNVDEAAAAGGN
                     DWSAKAAAMAAGEGAGSVVVSAAIEAELAGLPAEERAEYLTSLGLEEPGLNRVIREGY
                     ALLDLITFFTVGPKEARAWTVEKGATAPQAAGVIHTDFERGFIAAEVITFDDFAALGS
                     EQAVKEAGRMRLEGRDYVIRDGDVCHFRFNV"
     gene            complement(5517..5732)
                     /gene="infA"
                     /locus_tag="P5558_13295"
                     /pseudo
     CDS             complement(5517..5732)
                     /gene="infA"
                     /locus_tag="P5558_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
     gene            complement(5839..6048)
                     /locus_tag="P5558_13300"
     CDS             complement(5839..6048)
                     /locus_tag="P5558_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRY25333.1"
                     /translation="MSTGTVKWFNSTKGYGFIQPDNGTSDVFVHISAVEQAGMSSLNE
                     GQKVSFDVTTDPRRGKTSATNLRAV"
     gene            6430..7173
                     /locus_tag="P5558_13305"
     CDS             6430..7173
                     /locus_tag="P5558_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRY25334.1"
                     /translation="MRLGLALTLLLLQAPALAAVAQEATEYASARAGLVAQVELNAEL
                     AGPVTGHPVIAPSVLEAIREVPRHLFVPEAVAAYAYLDLPLPAAFGLRESQPFLVALM
                     SDLVDIQAGDDVLVLGVGGGYHAAVASRLAARVHAVDLDAAAATAVAARLERLGYGNV
                     EVRSADPYDGWPEAERTFDAIIVRLAVDRLPQALLRQLAPGGRLVAPIGPAESEQSLM
                     LVIRRADGSLGGRSVLPVRFMRLPGGERI"
     gene            complement(7198..7752)
                     /locus_tag="P5558_13310"
     CDS             complement(7198..7752)
                     /locus_tag="P5558_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY25335.1"
                     /translation="MSGASSNQAVSDDEARAAEAGAGEPAAATQEQRVFLVVVDESEE
                     MRTALRFACRRAQHTRGRVALLYVIEPAEFQHWLGVGRVMEEEARLEAERRMQTLAAE
                     VFAQTGSMPAVHIREGQRAEQLVALLDEEPSISLLVLGTATGGGDPGPLVTYLTSSAG
                     RNRLRVPVTLVPGHLSEAEIDALS"
     gene            complement(7756..8616)
                     /locus_tag="P5558_13315"
     CDS             complement(7756..8616)
                     /locus_tag="P5558_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HRY25336.1"
                     /translation="MTMAEAATGRSGGRSGDPLPGAGLQPGTLAGAPGRRLRRLGYAL
                     AGLALLGVVAGTARASGFIADLSHHLIAITTAFTGSNVLLFGALDDPGSDVVVVVRGP
                     DTSTAVRRKNRIGPVWLNTDEMTFSAVPSFYAIAASAPLGDIVAPDEQRRFRIGVGNL
                     GLRPEASGDRSALEISEFREALVRNKEKVGLYAAEIAPVRFLGDKLFRTTLAFPANVP
                     PGNYQIEVLQLRDGFVAGAQTSVLVISKVGLEAEIYDAARTQPAIYGLVSVILAVGAG
                     WSAGIFFRKQ"
     gene            complement(8652..9737)
                     /locus_tag="P5558_13320"
     CDS             complement(8652..9737)
                     /locus_tag="P5558_13320"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRY25337.1"
                     /translation="MIGLVAVAALLGLAVGWLLERSHFCTMGAISDAYLFGSLRRLRS
                     WLLAMATALLLTQTLAFSGLVPLDDTPYLARPLEPLALFIGGLLFGIGMVMAGGCASR
                     NLVRLGTGSLKALVVVLALGLTAMATAGGLLAWPADLLQAASAGSGQSLGGLLAGGIP
                     AGARALDFVLALLIGGGLLFWIFKDPGFRRNRADQMVGLGLGTAAAAGFLATGWLFLD
                     PAGQAGVVSLNFAGPMAEAVAWAATGQGTWPGFGAALVLGTVAGAAFSAGSAKRARIE
                     TFSSRDDMIRHVAGAGLMGFGGMLAMGCTIGQGVSGLATLAPGSFLALAGIVLGALLG
                     LRRLEHGSWRGAIGWGGGRRLEPAERI"
     gene            complement(9924..11189)
                     /locus_tag="P5558_13325"
     CDS             complement(9924..11189)
                     /locus_tag="P5558_13325"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY25338.1"
                     /translation="MGLYLAFSLVLLGGVATWVLQLRFAERDARLSELQQELDARTAD
                     HGRERQDLESTIAGLRHELRSDRDRLADMADENVALAAASRRQAAALAALSDEAELAR
                     QRASLLDLDARETALWLETAQRERMALERERAALQARFDALAMERDTARRQEEGLRRR
                     FGELEQHLASLSGEYALAASQMQDRIRDKLDAIERVVGRTGIDPQALLARAHDAADDG
                     QGGPLLGLDELQPSSGGDHAILGFDAARAGPAARAPAASFTNAFASELADELLRLDAV
                     PRLLEALPLDAPLADYRISSPFGVRTDPITGRRARHAGLDFGAASNSAVAATAPGRVV
                     AAGWRGGYGKLVEIDHGLGFTTRYAHLSRTLVAVGDRVAPGDPVGIIGSTGRSTGRHL
                     HYEIRLDGRPLDPVGFLAAGRDLPGILDG"
     gene            complement(11367..12461)
                     /gene="ugpC"
                     /locus_tag="P5558_13330"
     CDS             complement(11367..12461)
                     /gene="ugpC"
                     /locus_tag="P5558_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY25339.1"
                     /translation="MAEVVVSGIVKSFGKVAVLKEVSVAIADGEMLVVLGPSGCGKST
                     LLRIIAGLETASAGDVMIGGRRVNDLEPAERDIAMVFQNYALYPHMTVRQNMAYGLKN
                     LGTAKAVIEKRVAEAAQILGLAELLDRKPRQLSGGQRQRVAMGRAIVREPEAFLFDEP
                     LSNLDAKLRVQMRHEIKQLQRRLGTTGIFVTHDQVEAMTLGDRLLVLNQGVVEQVDTP
                     IEVYARPASLFVAGFIGSPAMNVLDGTIDGDGTTVTCGGARLRLEHELPGEAGREVRV
                     GIRPEHLAPIAAGAPSPDAGLDVEVEWVELLGADTVAHGRLADGQALALRRPGDRPVA
                     AGERFHLTPAPAHLHLFDPATGRRIDKGRR"
     gene            complement(12463..13311)
                     /gene="ugpE"
                     /locus_tag="P5558_13335"
     CDS             complement(12463..13311)
                     /gene="ugpE"
                     /locus_tag="P5558_13335"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HRY25340.1"
                     /translation="MVQNRPFFTVLVHLTLILGVLVVALPIYVTFIASTQAPSDVLRA
                     PIPMTMGDRLVENYSGVLGRGSMQASSGPVAPMMLNSLIMALGIAIGKIAISLLSAFA
                     IVYFRFPGRMLAFWTIFITLMLPVEVRILPTYEVMATLGLLNSYTGLIVPLIASATAT
                     FLFRQFFMTVPDELVEAARVDAAGPLRFFWDILLPLSRTNIAALFVILFIYGWNQYLW
                     PLLITTDQSMYTIVMAIQRMVVAADNEPEWHFITAVAILALIPPVVVVVLMQRLFVKG
                     LVETEK"
     gene            complement(13311..14195)
                     /gene="ugpA"
                     /locus_tag="P5558_13340"
     CDS             complement(13311..14195)
                     /gene="ugpA"
                     /locus_tag="P5558_13340"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HRY25341.1"
                     /translation="MEKRVVFRHRWLPYALLAPQLAITLVFFIWPAAQALWQSMLVQD
                     AFGLSVRFVWFDNFTYLFGNREYLHSIRVTAVFSLGVALLGLVVALLLAAMADHAIRG
                     AVVYKTLLIWPYAVAPAIAGVLWLFLFNPSVGIVAWWLQGIGIDWNHKISGNQAMLLV
                     IIAAAWKQVSYNFLFFLAGMQAIPRSLIEAAAIDCAGPGKRFFTIVFPLLSPTTFFLI
                     VVNIVYAFFDTFGIIHAVTRGGPAQATNIMVYKVFSDGFIGLNLGSSAAQSVVLMAIV
                     VTLTVVQFRFVERRVQYG"
     gene            complement(14251..>14568)
                     /locus_tag="P5558_13345"
     CDS             complement(14251..>14568)
                     /locus_tag="P5558_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646785.1"
                     /note="with UgpACE is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY25342.1"
                     /translation="TPEVQAWWHQETGYLPITTAAYELSKEQGFYEQNPGADVAIRQI
                     TLNPPTENSKGLRFGNFVQIRDIVAEELERVWAGDATAQEALDEVVRRGNELLRQFQQ
                     QQG"
BASE COUNT         2310 a         4969 c         5022 g         2267 t
ORIGIN      
        1 gtgccgcgga gctctcgatc gtcagcgggg cggtcggccg ggacatcgac gagctgcgca
       61 agcatctcga ctggttcgag gaacggacgg ggcacacggt ctccatcgtc accatgccgg
      121 cctcgacctc cgaccagttc ggccagtatc gcctctggct ctcggcgcag aaccccgata
      181 tcgacgtcta tcgcacggac gtcatctggg cgccgcagct cgccagccat ttcgtcgacc
      241 tgaccgagca cgtgcaggac gtcgtcggcg agcatttccc ggcgatcatc gaatcgcaga
      301 cggtggacgg caagatcgtg gcgctgccga tgttcaccga tgcgccggcc ctctactacc
      361 ggaccgatct gctcgagaag cacggcttcg agccgccgac cacctgggtc gggatggccg
      421 agacggcgca ggcggtgatg gaagccgagc gcgccgaggg caatgcggag atctggggct
      481 tcgtctggca gggctccgcc tatgaggggc tcacctgcaa tgcgctcgaa tgggtgaagt
      541 cgaacggcgg cggccagatc gtcgagccgg acggcgagat ctcgatcaac aacgagaacg
      601 ccgccgaggc gatcgacctg gccaggacct gggtcgacac catctcgccg cccggtgtcc
      661 tcggctacaa ggaggaggag gcccgcggcg tctggcaggt cggcaactcg gtcttcatgc
      721 gcaactggcc ctatgcctac gcgctgagca acggcgacga ttcggcggtc aaggacaagt
      781 tcgccgtggt gccgctgccg atcggcccga gcggcgaccg cagcgccgcc acgctcggcg
      841 gctggaatct cgccgtctcg gcctactcgc cggaccagga ggcggcgatc gagctggtca
      901 agttcctggc cagcgccgag gtccagaagg accgcgccat cgagacgacc cgcctgccga
      961 cgatccccgc gctctacgag gatgccgagg tggcggaggc ccagccggtc atcccgctct
     1021 ggctcgacat cttcctgaat gcggtgccgc ggccatcggc gccgaccaag gcgaactaca
     1081 accaggtgag ccaggagttc tggacggccg tccacaacac gctttcgggc acgggcgatg
     1141 ccgccaccaa cctcgcggag ctcgagcgca acctgaagcg catccgccgc tccggctggt
     1201 agttgggcga ggcatgatgc cggcgcgggc cgccggcatc atgtcgcctc ccgggtagag
     1261 aacggccccg gtgccggcac cggggtgacg caaccagatc gggcagggaa cgcaacatgg
     1321 ccgccgccac ctcccagctg actcgcgaga agatccgcgc tgcctggatc ttcctggtcc
     1381 cgatgatgct ggtgctgctg gcggtggccg gctggcccct gctgcggacg atctggctcg
     1441 gcttcaccga cgccaagctg tcgcagccgg gcagcgggca gtggatcggc ttcgagaatt
     1501 atctcttctg gctcgactac ggcgagggcg acggcgagtg gatcgggctg ctcgcggacc
     1561 cgacctggtg gcgcgccgtc tggaacacgg tctacttcac cgtcatcgcg gtgacgatcg
     1621 agacactgct cggcctcatc gtggccctgg tgctgaacgc cgaattcgcc ggccgcgggt
     1681 tcgtgcgcgc cgcgatcctg gtgccctggg ccattcccac catcgtctcg gcgaagatgt
     1741 gggactggat gatgaacgac cagttcggca tcctgaacga catgttcctg aagctcgggc
     1801 tgatcagtgc gcccattgcc tggaccgccc agcccgagac cgcgatgatc gccgtgatca
     1861 tcgtcgatgt gtggaagacc acgcccttca tggcgctctt gatcctcgcc ggcatgcaga
     1921 tgctgccggg cgacatctac gaggccgcga aggtcgacgg catccatccg gtcaaggtct
     1981 tcttcagggt gacgctgccc ttgatccgcc cggcgatcat ggtggcggtc atcttccgca
     2041 tgctcgattc cttgcggatc ttcgacctga tctacgtgct cacccccaac aatgcgcaga
     2101 ccgttaccat gtcggtctat gcaaggcagc acctcttcga tttcgacaat ttcgcctacg
     2161 gctccgctgc ttcgaccctg ctcttcctga tcatcgccgg catcaccatc ctctacatct
     2221 acgccggccg gctcaatctg agcggagaca agtgatgcgg aacaggctgc tcaagcaggt
     2281 cggcctctgg gtgctgatcg tcatcatctc ggtctacgcg ctcttcccat tctactatgc
     2341 gatcatcacc tcgttcaaat cgggctcggc catcttcagc gtgacctacc tgcccgacag
     2401 cttcagcctg gcgaactatg tcggcgtgct gggcagcggc cacttcccgc gcaacatcgt
     2461 caactcggtg atcgtcgcca ccgccgtggt gatcctctcc ttgctgctcg cgatcaccgc
     2521 gtcctatgcg ctcgcccgcg tgcgcttcaa gggccggggc atcctgctct tcaccatcct
     2581 ctcggtctcg atgttcccgc agatcgcggt cctcgccggg ctcttcgaga tggttcgcgg
     2641 cctcggcctc tacaacacgc tcttctcgct gatcctggcc tacatgatct tcaccctgcc
     2701 gttcaccgtc tgggtgctca cgacgttcat gcgcgacctg ccggtcgagc tggaggaggc
     2761 ggcgatcgtc gacggggcga agccgcatca gatcatcttc aaggtgttca tgccgctgat
     2821 gtggccagca ctggtgacca ccggcctcct ggccttcatc tcggcctgga acgagttcct
     2881 gttcgcgctg accttcgtct cgaacaacga gcagcgcacc gtgccggtgg cgatagcgct
     2941 cctctccggc gccaccgagc aggagatccc gtgggggccg atcatggcgg cgtcggtcat
     3001 cgtcacggtg ccgctcgtca tcctcgtcct cgtcttccag cgtcgcatcg tcgccggcct
     3061 gaccgccggc gccgtcaagg gctgatcccg gccgtgcctc gccaccggtc agtttgagaa
     3121 agagagaaat cgtcgatggc cacgatcaag ctggacagcg tgaagaaatc cttcggcaag
     3181 gtcgacgtga tcaagggcgt cgatctcgac atcaagagtc aggagttcgt cgtcttcgtc
     3241 gggccatccg gctgcggcaa gtcgaccctg ctgcggctgg tcgccgggct cgaggacatc
     3301 acctcgggca cgctcgccat cgacggtcgg gtcgtcaaca ccctctcgcc gtccgatcgc
     3361 ggcatcgcca tggtcttcca gtcctatgcg ctctacccgc acatgaccgt ctacgagaac
     3421 atggcgttcg gcctgaagct ggcgaagacc gacccgcgct cgatcgagga gcgggtcaac
     3481 gaggcggccc ggatcctgca gctcgacccc ctgctgcagc gcctgcccaa gcagctctcg
     3541 ggcggtcagc gccagcgcgt ggcgatcggc cgggccatcg tccgcaagcc caaggtcttc
     3601 ctcttcgacg agccgctctc caatctcgac gcggcgctgc gcgtgcagat gcgcatcgag
     3661 atcgccaggc tgcaccactc gctcgacgcg accatgatct acgtgaccca cgaccaggtc
     3721 gaggccatga cgctcgccga ccggatcgtc gtcctgcgcg acggctacgt catgcagttc
     3781 gggaccccgc tcgatctcta ccatcacccg gagaacaggt tcgtcggcgg cttcatcggc
     3841 tcgcccaaga tgaacttcct gccctgcgat ttggttggcg acaccgccgg gcaggcggtg
     3901 gtcgagactg tcgccggcac ccgggtctcg accacctcgg cgatcaacgg ccagcgcccg
     3961 ggcagcggct tcaccctcgg cgtccggccc gaggatctcc tggccggagg cggcggcgac
     4021 gccgagatcg agggcgaggt catcacggtc gagcatctgg gtgccgagac ctacatctat
     4081 gtcgatctcg gcatggagga gccgctcgtc gtcaaggccg ccggcaacag cgccgtgtcg
     4141 catggcgacc gggcccgcgt cggcattccg gccgccgcct gctacctgtt cgatccgaag
     4201 gagcgggccc tcgagcgacc cgcctacaac tagcggacag gcaggtccgc ctctcgggga
     4261 agctcagacg ttgaagcgga aatggcagac gtcgccgtcc ctgatgacgt agtcgcggcc
     4321 ctcgagccgc atcctgccgg cttccttgac cgcctgctcg ctgccgagcg cggcgaaatc
     4381 gtcaaaagtg atgacctcgg cggcgatgaa gccgcgctcg aaatcggtgt ggatgacgcc
     4441 ggctgcctgg ggtgcggtgg cccccttctc gacggtccag gcgcgcgctt ccttggggcc
     4501 caccgtgaag aaggtgatca ggtcgagcag cgcatagccc tcgcggatga cgcggttgag
     4561 gccgggctcc tcgaggccga ggctcgtgag gtactcggcc cgctcctcgg ctggcaggcc
     4621 cgccagctcc gcctcgatcg ccgccgacac gacgacgctg ccggcccctt cgccggcagc
     4681 catggcggcg gctttggcgc tccagtcgtt gccgcccgcg gctgccgcct cgtcgacatt
     4741 gaggacgtag agcaggggct tggcggtcag cagcgtgagg tctgcgacga gcagtcgctg
     4801 ctccggatcg agcggcagcc ggcgcaccgg ccggccctcc tccatcagcg gcaggatgtg
     4861 ctcgatcagc tcgagccggc gcttcgcctc cttgtcctgg gcgcggatgc gcttgacgag
     4921 gccgtccttc tgccggttca ggctgtcgat gtcggcgagc aggagctcgg tctggatcag
     4981 ctccgcgtcg cgcagcggat cgacgccgcc ctcgacatgg gtgatgtcgt catcctcgaa
     5041 gcagcgcagc acctcgagga tcgcatcgac ctcgcgaatg gcgccgagga acttgttgcc
     5101 gagcccctcg cccttggagg ccccgcggac gagaccggcg atgtccttga tctcgagctg
     5161 ggtcggcagg gtcttcgccg acttgccgag ctttgccagg agatcgaggc gcgggtcggg
     5221 caccggcagc cgcccgacat tgggctcgat ggtgcagaag ggataattgg ccgcggccgc
     5281 cgccgcggtc tgcgtcagcg cgttgaaaag ggtggacttg ccgacgttcg gcaggccgac
     5341 aatgccgcag ttgaagccca tgctggttct ggcgctcgtc tggatgaatg ggcgtcacga
     5401 aagtgcacgc gcccgagaaa tgcagagggg gtgcgcgacg agcgcgcaac agcccggttc
     5461 gctcagcggc gacgcggggg cgggcggcgg ttgccggtcg gggtggggcc cgccgtcttg
     5521 tgccggtagt tgatgcgacc cttggtcagg tcgtagggcg tcagctccag cgtcaccgga
     5581 tcgccggcga tgatccggat acggtgcttg cgcatccgcc cggaggcgta gccgatgatc
     5641 tcgtgcccgt tctcgagctt gacgcgaaac cgtgcgtccg ggagcacctc ggtgacggtg
     5701 ccctccatct cgatcatgtc ttccttcgcc attcgttctc cctggcccct gatgccgagc
     5761 gggccctcga gttctcggaa aagacgaaaa gggccggccg ttaacggccg gcccgatccg
     5821 gttcgcgacg cctgagtgtc agacggcgcg gagattggtc gcggacgtct tgccgcggcg
     5881 cgggtcggtc gtcacgtcga acgagacctt ctggccctcg ttcaggctgc tcatgccggc
     5941 ctgctcgacg gccgagatgt gcacgaacac atcgctggtg ccgttgtcag gctggataaa
     6001 gccgtagccc ttggtgctgt tgaaccactt tacggtgccg gtggacatat tccatcccca
     6061 agctgaagtt accgtggcga ccatcaccac ggcgtcaagc cacgtcggtc aggaagtcca
     6121 ggcaccctgc ggcacccctg gccggcaagg caaaaccatg ccggccgaaa caacttacct
     6181 tagcggatcg atggcgaaga taatggcgat ccacaactgc gattgcaagg gcgttttggt
     6241 tgaaggcccg cctcctgcgg cactcgttcg gctcgcggac aatgacggtg aagcggtcac
     6301 tggctgagtt cttgcaagga ccggcaccgg cggatgaagc gtccgggcat tgacgcggtg
     6361 ccttcggaag ggtttcatgg ccgaggcgag ctcgaccggt tgatcgatcc tttcccggga
     6421 gcgaaggaga tgcggctcgg cctggcgctc acgctgctgc tgctgcaggc cccggcactt
     6481 gcggcggtgg cgcaggaggc gacggaatac gcttcggccc gggccgggct ggtggcccag
     6541 gtcgagctga atgccgagct cgcggggccc gtcaccggcc acccggtcat cgccccaagc
     6601 gtcctcgaag ccatccgcga ggtgccgcgg cacctgttcg tgcccgaggc ggtggcggcc
     6661 tatgcctatc tcgacctgcc gctgccggcg gcgttcggcc tgcgcgaatc ccagcccttc
     6721 ctcgtcgccc tgatgtccga tctggtggat atccaggcgg gcgacgacgt gctcgttctg
     6781 ggtgtcggtg gcggctatca cgcggcggtg gcgagccggc tcgcagcccg cgtccacgcc
     6841 gtcgatctcg atgcggcggc cgcaacggcg gtggcggccc ggctcgagcg gctcggctac
     6901 gggaatgtcg aggtgcgcag cgccgatccc tatgacggct ggccggaagc cgagcggacc
     6961 ttcgatgcga tcatcgtccg cctcgccgtc gaccgcctgc cgcaggccct gcttcgccag
     7021 ctggcgccgg gcggtcggct ggtcgcgccg atcggcccgg cggagagcga gcagtcattg
     7081 atgttggtga tccggcgcgc ggacggctcg ctcggcggcc ggtcggtcct gcccgttcgc
     7141 ttcatgcgat tgccgggcgg cgagcggatc tgaacggctc gtccggttcc ctcgcgctca
     7201 gctcagcgcg tcgatctcgg cctcgctcag atggccgggc acgagcgtca ccggcacgcg
     7261 cagccggttg cggccggcgc tgctggtcag ataggtcacg agcggcccgg gatcgccgcc
     7321 gccggtcgcg gtgccgagca cgaggagcga gatcgacggc tcctcgtcga gcagggcaac
     7381 gagctgctcg gcgcgctggc cctcgcggat atgcacggcc ggcatcgatc cggtctgggc
     7441 gaagacctcg gccgccaggg tctgcatccg ccgctcggcc tcgagccgcg cctcctcctc
     7501 catcacccgg ccgacgccga gccagtgctg gaactcggcc ggctcgatca cgtagagcag
     7561 ggcgacgcgc ccgcgcgtgt gctgggcgcg gcggcaggcg aaacggagcg ctgtccgcat
     7621 ctcctcggat tcgtcgacca cgacgagaaa gacgcgctgc tcctgggtgg cggcagcggg
     7681 ctcgcccgcc ccggcctccg ccgcccgggc ctcgtcgtcc gataccgcct ggttgctgct
     7741 ggcaccgctc atggctcact gctttctgaa gaagatgccc gcggaccagc ctgccccgac
     7801 cgcgaggatg accgagacga ggccgtagat cgccggctgg gtgcgtgccg cgtcgtagat
     7861 ctcggcttcg agcccgacct tcgagatcac cagcacgctg gtctgcgccc cggccacgaa
     7921 accatcccgc agctggagca cctcgatctg gtagttgccc ggcggcacgt tggccgggaa
     7981 ggcgagggtc gtccggaaga gcttgtcgcc gagaaagcgg accggtgcga tctccgccgc
     8041 atagaggccg accttttcct tgttgcggac gagggcctcg cggaactcgc tgatctccag
     8101 ggcggaccga tcgccgctgg cctcggggcg caggccgaga ttgccgacgc cgatgcgaaa
     8161 ccgtcgctgc tcgtcgggcg ccacgatgtc cccgagcggc gcgctcgccg cgatggcata
     8221 gaaggacggc acggcggaga aggtcatctc gtcggtgttc agccagacgg ggccgatgcg
     8281 gttcttgcgc cgcacggccg tgctggtgtc cggcccgcgc acgaccacca ccacatcgct
     8341 gccgggatcg tcgagcgcac cgaacagcag gacattgctg ccggtgaagg cggtggtgat
     8401 ggcgatcagg tgatgcgaca ggtcggcaat gaagccgctg gcccgggccg tcccggccac
     8461 gactccgagc agggcgagac cggcgagcgc gtagccgagc cgccgcaggc gccgacccgg
     8521 cgccccggcg agcgtgcctg gctgcaggcc ggcacccggc aggggatcac ccgacctgcc
     8581 gcccgatcgc cctgtcgccg cctctgccat cgtcatcctt tgtcccgcgc cgggtcgacc
     8641 ccggaccttg ctcaaatccg ctccgccggc tccagccgcc gaccgccgcc ccagcctatc
     8701 gcaccacgcc agctgccgtg ctcgagacgc cgcaggccga gcagcgcacc cagcacgatg
     8761 ccggccagcg ccaggaacga gcccggggcc agggtggcga ggccgctcac gccctggccg
     8821 atcgtgcagc ccatggccag catgccaccg aagcccatga gcccggcgcc cgccacgtgg
     8881 cgtatcatat cgtcgcggct cgagaacgtc tcgattcgcg cgcgcttggc gctgccggcg
     8941 gagaaggcgg cgccggcgac ggtgccgagc accagcgcag caccgaatcc gggccaggtg
     9001 ccctggcccg ttgcggccca ggcgacggcc tcggccatcg ggcccgcgaa attgagcgag
     9061 acgacgccag cctgcccggc ggggtcgagg aagagccagc ccgtcgcgag aaaacccgca
     9121 gcagcggctg tgcccagccc gaggccgacc atctggtcgg cgcgattgcg gcgaaagccg
     9181 ggatccttga agatccagaa gagcaggccg ccgccgatga gcaaagcgag gacgaagtcg
     9241 agggcccgcg cccccgccgg gatgccgccg gccaggagcc cgccgaggct ctgcccggaa
     9301 ccggccgaag ctgcctgcag caggtcggcc ggccaggcga gtaggccgcc ggcggtggcc
     9361 atggcggtga ggccgagcgc caggacgacg accagcgcct tgaggctgcc ggtgccgagg
     9421 cgcaccagat tgcggctggc gcagccgccg gccatgacca tgccgatccc gaagaggaga
     9481 ccgccgatga agagcgccag cggctccagg ggccgtgcca gataaggcgt gtcatcgagc
     9541 ggcacgaggc ccgagaacgc cagcgtctgg gtcaggagca gggcggtcgc catcgcgagc
     9601 agccagcttc gcagccggcg caggctgccg aagagatagg cgtcgctgat cgcgcccatg
     9661 gtgcagaaat ggctgcgctc gagcagccag ccgaccgcca gcccgagcag cgccgccacg
     9721 gccacgagcc cgatcaagac aggctccgcc cgatcgtcac gactccgacc tgccatagcc
     9781 ccgcggtcgg ggcatggcgg tcgctcgtgc gacacgactg gtggttatcg ggcagctgac
     9841 gggccatgcc atagcatagc cgcaacctgc cgacctgcca atcggccgcc gacccgccga
     9901 cgggctgcgg gtgaggtgcc gtgtcagccg tccagaatgc cgggcaggtc gcggccggcc
     9961 gcgaggaagc cgaccgggtc gagggggcga ccgtccaggc ggatctcgta atgcaggtgc
    10021 cgcccggtgc tccggccggt gctgccgatg atgccgaccg ggtcgcccgg ggcgacccgg
    10081 tcgccgaccg cgaccagcgt ccgcgagaga tgggcatagc gggtcgtgaa gccgagaccg
    10141 tggtcgatct cgacgagctt gccatagccg ccacgccagc ctgccgcgac cacgcggcca
    10201 ggtgccgtgg cggcgacggc gctgttcgag gcggcgccaa agtcgaggcc ggcatgcctg
    10261 gcgcgccggc cggtgatcgg gtcggtcctg acaccgaaag ggctcgagat ccgatagtcc
    10321 gccagcggtg cgtcgagcgg caacgcctcg agcaggcgcg gcaccgcgtc gagccgcagg
    10381 agctcgtcgg cgagctcgct tgcgaaggcg ttggtgaagg aggcggcggg tgctctggcc
    10441 gccgggccgg cgcgcgccgc atcgaagccc aaaatggcgt gatcgccgcc ggatgacggc
    10501 tgcagctcgt cgaggccgag cagcggcccg ccctggccgt cgtccgcggc atcatgagcc
    10561 cgggccagga gcgcctgcgg atcgatcccg gtccggccga ccacccgctc gatggcgtcg
    10621 agcttgtccc tgatccggtc ctgcatctgg ctggcggcca gggcgtactc gccggagagc
    10681 gacgcgagat gctgctccag ctcgccgaaa cgccggcgca ggccctcctc ctgccggcgg
    10741 gcggtgtcgc gctccatggc cagcgcgtcg aatcgcgcct gcagcgccgc cctttcgcgc
    10801 tcgagcgcca tgcgctcgcg ttgcgcggtc tcgagccaga gcgccgtctc gcgggcatcc
    10861 agatcgagga ggctggcgcg ctggcgggcc agctcggctt cgtccgagag tgccgcgagc
    10921 gctgcagcct gccggcggct cgcggcagcc agagccacat tctcgtccgc catgtctgcg
    10981 aggcgatccc ggtcgctgcg cagctcgtgc cggagccccg cgatggtcga ttcgaggtcc
    11041 tggcgctcac gcccatggtc cgccgtgcgg gcgtccagct cctgctgcag ctcgctcagg
    11101 cgggcgtcgc gctcggcgaa gcggagctgc agcacccagg tcgcgacgcc gccgaggagg
    11161 acaaggctga aggccagata gagtcccacg gcaacgagca tccgccgcga cgggccgccc
    11221 ggtgccgccg acgagacggg ggaacccgct ggctcggcgg ccgagctgct ggtcggggcg
    11281 gacgaacgca tcggcctctc ttcgctccat gacggcgttg tcgtatcggt ggcgccgact
    11341 atgcacgagg catggttgcg aggccgttaa cgccggccct tgtcgattcg acggccggtc
    11401 gccgggtcga agagatggag atgggctggg gccggggtca gatgaaagcg ctcgccggcc
    11461 gccaccggcc ggtcgccggg ccggcgaagg gccagcgcct ggccgtcggc cagccggccg
    11521 tgcgccactg tatcggcacc caggagctcg acccattcca cctccacgtc gagcccggca
    11581 tccggcgatg gcgcgcccgc agcaattggc gccagatgct ccgggcgaat gccgacccga
    11641 acctcgcgcc cggcctcgcc cggcagctcg tgctccaggc gcaggcgcgc cccgccgcag
    11701 gtcacggtgg tgccgtcgcc gtcgatcgtg ccgtcgagca cgttcatcgc gggcgagccg
    11761 atgaagccgg cgacgaacag gctcgccggc cgcgcgtaga cctcgatggg ggtgtcgacc
    11821 tgctcgacca cgccctggtt cagcacgagg agccggtcgc cgagcgtcat ggcctcgacc
    11881 tggtcgtggg tgacgaagat gccggtcgtg ccgagccggc gctgcagctg cttgatctcg
    11941 tggcgcatct gcacccggag cttggcgtcg agattggaga gcggctcgtc gaagaggaac
    12001 gcctcgggct cgcggacgat cgcccggccc atggcgaccc gctggcgctg gccgcccgag
    12061 agctggcggg gcttccggtc gagcagctcg gcgagcccca ggatctgcgc cgcctcggcc
    12121 acgcgctttt cgatgacggc cttcgccgtg ccgaggttct tcaggccgta ggccatgttc
    12181 tggcggacgg tcatatgcgg gtagagcgcg tagttctgga agaccatggc gatgtcgcgc
    12241 tcggcgggct cgagatcgtt gacccgccgg ccgccgatca tgacgtcgcc ggcgctggcg
    12301 gtctcgagcc cggcgatgat ccgcaggagc gtggacttgc cgcagcccga ggggccgagc
    12361 acgaccagca tctcgccgtc ggcgatcgcc accgagacct ccttcagcac ggcgaccttg
    12421 ccgaacgact tgacgatccc gctgacgacg acctcggcca tgctacttct cggtctccac
    12481 caggcccttg acgaagagcc gctgcatgag aaccacgacg acgaccggcg ggatcagcgc
    12541 caggatcgcg acggcggtga tgaaatgcca ctcgggctcg ttgtcggccg ccaccaccat
    12601 gcgctggatc gccatgacga tcgtgtacat gctctggtcg gtggtgatca gcagcggcca
    12661 gagatactgg ttccagccgt agatgaagag gatgacgaag agggcggcga tgttggtccg
    12721 ggagagcggc aggaggatgt cccagaagaa ccggagcggg cccgccgcat cgaccctggc
    12781 cgcctccacc agctcgtccg gcacggtcat gaagaactgg cggaagagga aggtcgccgt
    12841 cgccgaggcg atcagcggca cgatcaggcc ggtgtaggaa ttgagcaggc cgagcgtcgc
    12901 catgacctcg taggtcggca ggatgcgcac ctcgaccggc agcatcaggg tgatgaagat
    12961 ggtccagaag gcgagcatcc gcccggggaa gcggaagtag acgatggcga aggccgagag
    13021 cagcgagatc gcgatcttgc cgatggcgat gccgagcgcc atgatcagcg agttgagcat
    13081 catcggcgcc accgggccgg agctcgcctg catgctgccc cggccgagca cgcccgagta
    13141 gttctcgacc agccggtccc ccattgtcat gggaatcggg gccctgagca catctgacgg
    13201 cgcctgggtc gaggcgatga aggtgacgta gatcggcagc gccacgacga gcacgcccag
    13261 gatcagcgtc aggtggacga ggacggtgaa gaacgggcgg ttctggacca tcagccgtac
    13321 tgcacccggc gctcgacgaa gcggaactgg acgacggtga gggtgacgac gatcgccatc
    13381 agcaccaccg actgcgccgc cgagctgccg aggttgaggc cgatgaagcc gtcgctgaag
    13441 accttgtaga ccatgatgtt ggtggcctgg gccggcccgc cgcgggtgac ggcgtggatg
    13501 atcccgaagg tgtcgaagaa ggcgtagacg atgttgacga cgatcaggaa gaacgtcgtc
    13561 ggcgacagca gcgggaagac gatcgtgaag aagcgcttgc ccggcccggc gcagtcgatc
    13621 gccgccgcct cgatcagcga tctcgggatc gcctgcatgc cggccaggaa gaagaggaag
    13681 ttgtaggaga cctgcttcca ggcggcggcg atgatgacca gcagcatggc ctggttgccg
    13741 ctgatcttgt ggttccagtc gatgccgatc ccctgcagcc accaggcgac gatgccgacc
    13801 gaagggttga agaggaagag ccagagcacg ccggcgatcg ccggggccac ggcgtagggc
    13861 cagatcaaga gcgtcttgta gacgacggcg ccgcggatcg cgtgatcggc catggcggcg
    13921 aggagcaggg ccacgacgag gccgagcagc gccacgccga ggctgaagac cgccgtcacc
    13981 cggatcgagt gcaggtactc gcgattgccg aagagatagg tgaagttgtc gaaccagacg
    14041 aagcgcacgg agagcccgaa ggcgtcctgc accagcatcg actgccagag cgcctgggcc
    14101 gccggccaga tgaagaagac gagggtgatc gcgagctgtg gcgccagcag cgcataaggc
    14161 agccagcgat gacgaaagac gacgcgcttc tccatgcgcg gggcaaccgc ggtcgaggag
    14221 cgccggcgcg ctcctcgacc gtcggatcgc tcagccctgc tgctgctgga actggcgcag
    14281 cagctcgttg ccgcgcctga ccacctcgtc gagcgcctcc tgcgccgtcg cgtcgccggc
    14341 ccagacccgc tcaagctcct cggcgacgat gtcgcggatc tgcacgaagt tgccgaagcg
    14401 cagacccttc gagttctcgg tcggcgggtt cagcgtgatc tgccggatgg cgacgtcggc
    14461 gccgggattc tgctcgtaga atccctgctc cttgctgagc tcgtaggccg ccgtggtgat
    14521 cggcaggtag ccggtctcct gatgccacca cgcctgcacc tcgggcgt
//
LOCUS       DAOTFI010000121        14508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_4991_length_14508_cov_7.717152, whole genome shotgun sequence.
ACCESSION   DAOTFI010000121 DAOTFI010000000
VERSION     DAOTFI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14508
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4991_length_14508_cov_7.717152"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="P5558_13350"
     CDS             <1..390
                     /locus_tag="P5558_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRY25343.1"
                     /translation="TASFCGYTDQYAELQEIWERYGDRGLVVLGVPSDDFNQELDSDA
                     AVKEFCEVEFGVTFPLTTVSHVRGPASHPLFAHFRDVLGDGAGPSWNFNKYLIAPTGE
                     VVAAWPARTRPGGPEITGAIERFLPAS"
     gene            443..2191
                     /locus_tag="P5558_13355"
     CDS             443..2191
                     /locus_tag="P5558_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY25344.1"
                     /translation="MSGRRLPWPSWRGLRLGSGLVLMAFVTTHLLNHAFGLVSFEAMD
                     PAREWLGFWHRAEIWPILLAAFILHILAALWSLYERRGLRMAPWQYLQIASGLLIPLF
                     LALHYIGSRGIFETLGIPIGYEFELFVIWPAYAWQQSILLVLVWLHGCIGVHYWLRLH
                     GWYRRIAGPLLALAVLVPTLSLAGFISASREFHTRAAAEPQWLAALARAERWPADPAE
                     LAFVYQGEKLIIGVFLALVLLALVARSARAFWRERRNSFAVTYAGGRRVRAQRGASLL
                     EVSRLAGIPHASVCGGRGRCSTCRVRITAGGDALPPPAPAEERVLQRLGAAPEVRLAC
                     QTRPKGDVSLALLMPAEATSREALRPMNPGHGIEREIAVLFADLRGFTRLSEGRLPFD
                     VVHLLNRYFKTMGAAIDDAGGHVDKFIGDGIMALFGLDTDAETAARQALRAARAMGAA
                     LDGLNQEFRTELPQPLRMVVGLHLGTTIVGELGYGRSTSLTAIGDAVNVASRLEGVAK
                     EQDMAFVISADLLNRAGVAADAALLRDVTLRGRAEPLHVVLFATPVELPDLPAATSPR
                     SLVARLFSRPSPAPAG"
     gene            complement(2160..3068)
                     /locus_tag="P5558_13360"
     CDS             complement(2160..3068)
                     /locus_tag="P5558_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRY25345.1"
                     /translation="MNLRDLDYLVAVADTGHFGLAADRCRVSQPTLSMQVKKLEAELG
                     TALFERSPRGALTTAIGAAIVARARHVQEEVRQMRELAARGAAGFVGMLRLGVIPTAG
                     PYILPRILPVIQATYPRLEAHLREAMTARLLALLHANELDAAIVSEPIDEPGLDRADL
                     LRERFMLTASPAQAAALAGPDRAAALAGAPVLMLEDGHCLKDQTVLVCRRLGLQPVLR
                     VQASSVETLRQMVALDYGLAVLPELATRGRFAGPGSGAGAVVAEPLAGADCSRQLVLV
                     WRRTFPRPEQLQTLARLIRQELATDG"
     gene            3248..>3792
                     /locus_tag="P5558_13365"
     CDS             3248..>3792
                     /locus_tag="P5558_13365"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRY25346.1"
                     /translation="MSDRPILTTTAGNPIADNQNSLTAGARGPVLMQDYQLIEKLAHQ
                     NRERIPERPVHAKGWGAHGTLTITHDITKYTRAAPFAKAGKQTPMIARFSTVAGELGA
                     ADAERDVRGFALKFYTEEGNWDLVGNNTPVFFVRDPYKFPDFIHTQKRHPRTNLRSPT
                     AMWDFWSLSPESLHQVTILMS"
     assembly_gap    3793..3892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3893..4875
                     /locus_tag="P5558_13370"
     CDS             <3893..4875
                     /locus_tag="P5558_13370"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRY25347.1"
                     /translation="FWSLSPESLHQVTILMSDRGLPTDVRHINGYGSHTYSFINAQNE
                     RFWVKFHFKTMQGHRHWTNAEAAEVIGRTRESTQEDLYAAIEKGDFPKWKVQVQIMPE
                     TDAEKTPYNPFDLTKVWPHGDYPPIDVGVLELNRNPDNYFAEIEQAAFAPSNIIPGIG
                     FSPDKMLQARIFSYADAHRYRLGTHYEALPVNHAKCPVHHYHKDGPMRFFDNNTKNPD
                     AYYEPNSFNGPAQAPSYAEPPLRISGDADRYDHRAGNDDYKQPGDLFRLMNAEQQGRL
                     CQNIAEAMGGVPENIVQRQLAHFYKADPAYGDGVAKALGVTFKPSTMAAE"
     gene            complement(4950..6518)
                     /locus_tag="P5558_13375"
     CDS             complement(4950..6518)
                     /locus_tag="P5558_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRY25348.1"
                     /translation="MTTYDLSDDSVVVVIGSGAGGGTLANELAQKGIDVVLLEAGKEQ
                     SIESFVNDEWASFAQLAWLDPRTTSGTWRVARDFAGLPTWICKTVGGTTTHWAGASLR
                     IREHEFKARTTYGEIAGATLLDWPLSLADLEPYYDKAEDKMGVTRTHGIPGLPGNNNF
                     KVMWNGATRLGYQKVHTGRMAINSEPRHDRNACHQLGFCFQGCKMGAKWSTLYTEIPA
                     AIDTGHCELRSQAFVLQIEHDASGKVTGVIYADADGNQQRQAARAVCVAGNSIESPRL
                     LLNSASSMFPDGLANSSGEVGRNYMRHTTGSVYAAFGQPVHMYRGTIMAGIIQDEAEN
                     RPERGFAGGYELETQSLGVPFMAAFLDPGAWGASFTRKMDQYANMAGLWIVGEDMPQS
                     SNAVTLHATMKDQHGLPVPNVHFDDHVNDIAMREHAFRQGRAVYEAAGATDIYEVPPY
                     PSTHNLGTNRMSEKPADGVVNAFGQSHDIDNLFVSDGSVFTTGAAENPTLTIVALAIR
                     QADYIAEQMGQGAI"
     gene            complement(6515..7087)
                     /locus_tag="P5558_13380"
     CDS             complement(6515..7087)
                     /locus_tag="P5558_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HRY25349.1"
                     /translation="MTKMILDRRRFLARTGQAMLVFGASGTVMFTDAERSWAMALDRL
                     DAPTAETLLRMCRALYPHDALGDMYYAVVVEALDSEAAGDPATATLISEGVGRLDAAL
                     GVPFAALSPGSQTEVLTLMQADPFFQKVRGAVVFHFYNNPLVWRHFGYEGASFEQGGY
                     VWRGFQDAGWLIEPDAEASPPVAYGEGAQP"
     gene            complement(7117..7512)
                     /locus_tag="P5558_13385"
     CDS             complement(7117..7512)
                     /locus_tag="P5558_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY25350.1"
                     /translation="MPKAIHMMVRVLELDRSIAFYGKALGLTVADRFDFETFTLVYLR
                     NAEADFEIELTLNKDRTAPYALGDGYGHVAFCVDDLDAEHERCAGLGLEPGKIVAFDR
                     DGGLMARFFFLTDPDGYRIEVLQRHGRYR"
     gene            complement(7567..7860)
                     /locus_tag="P5558_13390"
     CDS             complement(7567..7860)
                     /locus_tag="P5558_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRY25351.1"
                     /translation="MCHIYASVPPEAYEPVTRSVRLHGQVTSIRLEARFWEIIDTMAA
                     GQGMSTPRFVTTLYDEVLELRGTVGNFTSLLRVVCTVHLGACGEARAGLVVAE"
     gene            8127..10355
                     /locus_tag="P5558_13395"
     CDS             8127..10355
                     /locus_tag="P5558_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRY25352.1"
                     /translation="MLSLLVLLLVASTMPAKAQPAADADSAAIVAALETPDGDLSPEA
                     AAALVARLSDREVRSLLLERLAATGAAGEAGAGSPAGMIGSLTDRSSLVRERLGELLG
                     AVEDVPAAIATAWHRFHDGRSTGAMLALAAGVAISLLAAALAEALTRRRLARTRARLV
                     GSEARTQDLLNRSLHLLGMLALDLLGLLVFAVVAMVVLLAVTESHGPTRSAVLGFLGA
                     LVAFRLVVTLSHFLLNPGNKRHRMNDLADADASFLHRWMKVIAAIGTFGFVGCALVAL
                     LGEPGAEHDLLLNATGALLVASVIYAVLKGRRALTNSILVFDVSSPMRRVLAEMTPTL
                     TAITVFLIFVLYTVSSLLGVMLPPAAGLLTIAILVLVPYVDAVLERSAVYRRRYGGEA
                     ADAGGTVATVIIRGARLLLWIASFLVLAEVWSVDLRAAATAGVGDRISDALVDIGFAW
                     FAAYLGWEFARIAIDRRIASEAAPTTPGEHGDEGGTGASRLATLLPLAKRAIQITIGV
                     ITVMITLSALGVDIGPLLAGAGVVGIAVGFGAQTLVRDVVSGAFFLLDDAFRVGEYVD
                     VGSVKGTVEKISVRSFRLRHHRGALHTVPFGEIRQLTNYSRDWVIMKLEFRVPFDTDP
                     DRVRKIFKQIGQDLLANPELGPDFIEPFKSQGVFTLDDSALVIRGKFMAKPGKQFVAR
                     REIYNAVQRAFAAAGIRFADRRVTVQVADGEGMTTEERQRVAQAAASALQPPAAAAAS
                     GS"
     gene            complement(10359..11288)
                     /gene="htpX"
                     /locus_tag="P5558_13400"
     CDS             complement(10359..11288)
                     /gene="htpX"
                     /locus_tag="P5558_13400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRY25353.1"
                     /translation="MNALRTGILLAAMTGLFLAVGFVLGGEGGLLIALLLALGMNFFA
                     YWNSASMVLRMHNARVVTRASAPEYYGVVEGLAKRAGLPMPQVYVIETDQPNAFATGR
                     SPDHAAVAATTGLLRSLSREELAGVMAHELAHIKNRDTLTMTVAATIAGAIGFLANFA
                     MLFGAGRGENRPNPIAMILIMILAPMAAGLVQMAISRTREYSADRDGAAICGHPRWLA
                     GALLKIEQLARGRVMQTAEHNPASAHMFIINPLIKGGIDNLFRTHPATEERVRRLLAM
                     EGGSTSSGLGSRGPTPVSRGVRRAGSVPSAGRG"
     gene            11535..13856
                     /locus_tag="P5558_13405"
     CDS             11535..13856
                     /locus_tag="P5558_13405"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRY25354.1"
                     /translation="MDADEPVIPRSIVDSHEPVTSEEALELHRRGRPGKIEVTPTKPL
                     ATSRDLSLAYSPGVAAPCLAIQADPVRAYEYTAKGNLVAVISNGTAVLGLGNLGALGS
                     KPVMEGKAVLFKRFADIDGIDLEVDTEEVDAFVDCVKLLGPTFGGINLEDIKAPECFV
                     IEQRLRELMDIPVFHDDQHGTAIIAAAGLINALDLTGRSIATARMVVNGAGSAGIACA
                     ELIKAMGMPHENVTICDTKGVIYQGRTEGMNQWKSAHAVPTEARTLADAMAGADVVMG
                     LSAKGAFTNEMIASMAPNPIIFAMANPDPEITPEDAKAIRDDVIIATGRSDYPNQVNN
                     VLGFPYIFRGALDVRASTINDAMKIAAAYAIAALAREEVPEEVTAAYRGRRLRYGPDY
                     MIPTPFDPRLITHVPAAVAKAAVESGVAMKPIVDFERYRHELRSRLDPTASRMQLIID
                     RVRAHPKRIVFAEGEEEKAISAALAWRNGGLGLPILVGREERIRDTAKGMGQSSLDGI
                     EIHNARLSPHNRLYADFLYERNQRNGLLFRDCQRLVNQDRNVFGACLVACGHADAMVT
                     GLTRRYNTVIKDIRLVINPLPRKRMFGVTIIIARGRTVFIADTTVHELPDPEVCADIA
                     IQTAEVAEKMGFTPRVAFLSFATFGNPRISKAARVRDSVRALDERQVDFEYDGEMTAD
                     VALDPELMALYPFCRLSGPANVLIMPALHTAHISAKLLQQLGGGTVIGPLLIGLEKPV
                     QIVPMNASVSDILNVAALAAHDAQAIEAEAAAG"
     gene            13866..>14508
                     /locus_tag="P5558_13410"
     CDS             13866..>14508
                     /locus_tag="P5558_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY25355.1"
                     /translation="MPNIVAVTPGFHRIPLPVVLSDSTHGLMSAFELITVRITDAEGA
                     EGVGYTYTVGRNGGAIMDTLAREIEELVIGDDADRIEALWQKLWWALHYGGRGGPTVL
                     ALSAFDMALWDLKARRAGLPLWKLLGGHDPRVTCYAGGIDLDLPLDALLAQTDGNLSR
                     GFRAIKMKVGRPKLSEDTARIAAMREHLGDGFPLMVDANMKWTADQAIRAARAF"
BASE COUNT         2202 a         5178 c         4772 g         2256 t
ORIGIN      
        1 accgcctcct tctgcggcta caccgaccaa tatgccgagc tgcaggagat ctgggagcgc
       61 tacggcgatc gcggcctcgt cgtgctgggc gtgccctccg acgacttcaa ccaggagctg
      121 gacagcgatg cggcggtcaa ggaattctgc gaggtcgagt tcggggtcac ctttccactg
      181 acgacggtga gccacgtgcg cggtccggcg agccacccgc tcttcgcgca tttccgtgac
      241 gtgctgggcg acggtgccgg gccgagctgg aacttcaaca agtacctgat cgcgccgacc
      301 ggcgaggtcg tcgccgcctg gcccgcgcgg acgcggcccg gcggtccgga gatcaccggt
      361 gccatcgagc gttttctgcc cgcctcttga gttgccggcc cgcgggtcct aagagccgcc
      421 cacaatgcct gcggggtcga ggatgagcgg acggcgcctg ccatggccga gctggcgcgg
      481 cctgcgcctc ggctcgggcc tcgtgctgat ggccttcgtc accacgcacc tgctcaacca
      541 cgccttcggc ctcgtgtcgt tcgaggcgat ggacccggcg cgcgaatggc tcggcttctg
      601 gcatcgcgcg gaaatctggc cgatcctgct cgcggccttc atcttgcaca tcctggccgc
      661 cctctggtcg ctctacgagc gccgcggcct gcgaatggcc ccgtggcagt acctccagat
      721 cgcctcgggc ttgctgatcc cgctcttcct cgccctgcac tacattggca gtcggggcat
      781 cttcgagact ctcggaattc ccatcggcta cgagttcgag ctcttcgtca tctggccggc
      841 ctatgcctgg cagcagtcga tcctgctcgt cctcgtctgg ctgcacggct gcatcggcgt
      901 gcattactgg ctccggctgc acggctggta tcggcggatc gccggcccgc tgctcgcctt
      961 ggccgtgctc gtgccgaccc tctcgctcgc cggcttcatc agcgcgtccc gcgaattcca
     1021 cacgagggcg gcggccgagc cgcaatggct cgcggccctc gccagagccg agcgctggcc
     1081 tgcggacccg gcggagctgg ccttcgtcta ccagggcgag aagctgatca tcggcgtctt
     1141 tctggctctc gtccttctgg cgctcgtcgc gcgctccgcc cgggccttct ggcgcgagcg
     1201 gcgcaacagc ttcgccgtca cctatgccgg tgggcgacgg gtgcgggccc agcgcggcgc
     1261 cagcctgctc gaggtcagcc ggctcgccgg catcccgcac gcctcggtct gcggcgggcg
     1321 aggccgctgc tccacctgca gggtgcggat tacagccggc ggcgacgccc tgccgccgcc
     1381 ggccccggcg gaggagcgcg tgctgcagcg cctgggtgcg gcacccgagg tgcgcctcgc
     1441 ctgtcagacc cggcccaaag gcgacgtgtc gctggcgctc ctgatgccgg ccgaggcgac
     1501 gtcgcgcgag gcgctccggc cgatgaaccc cggccatggc atcgagcgcg agatcgccgt
     1561 gctcttcgcc gatctccgcg gcttcacgcg gctcagcgag ggccgcctgc cgttcgacgt
     1621 ggtccacctg ctcaatcgct acttcaagac catgggggcg gcgatcgacg atgccggcgg
     1681 gcatgtcgac aagttcatcg gcgacgggat catggcgctc ttcggcctcg acaccgatgc
     1741 ggagaccgcc gcccgccagg cgctcagggc ggcccgggcg atgggtgcgg ccctggacgg
     1801 tctcaaccag gaattccgga ccgagctgcc gcagccgctg cgcatggtgg tcggcctgca
     1861 tctcggcacc accatcgtcg gcgagctcgg ctatggccgg agcacgagcc tcaccgcgat
     1921 cggcgatgcc gtcaacgtgg cgagccgcct cgaaggggtc gccaaggagc aggacatggc
     1981 cttcgtcatc tcggccgacc tgctgaatcg ggccggcgtc gcggccgatg cggcgttgct
     2041 gcgggacgtc acgctgcgcg gccgggccga gcccctgcat gtagtgctgt tcgccacccc
     2101 ggttgagctc cctgacctgc ccgccgccac gtcgccgcgc tcgctggtgg cgcgcctctt
     2161 cagccgtccg tcgccagctc ctgccggatg agccgggcca gggtctggag ctgctccggg
     2221 cgcgggaacg tgcggcgcca gacgaggacg agctgccgcg agcaatcggc gcccgccagg
     2281 ggctccgcca ccaccgcccc ggcgccggag cccgggccgg cgaaccggcc gcgcgtggcg
     2341 agctccggca gcacggccag gccgtagtcg agggccacca tctggcgcag cgtctcgacg
     2401 ctgctcgcct gcacccggag caccggctgc aggccgaggc ggcggcagac gagcaccgtc
     2461 tggtccttca ggcagtggcc atcctccagc atcaggaccg gcgcgccggc cagggccgcg
     2521 gcccggtcgg gaccggcgag agctgccgcc tgcgccggcg acgcggtcag catgaaccgc
     2581 tcgcgcagga ggtcggcgcg gtcgaggccg ggctcgtcga tcggctcgga gacgatcgcc
     2641 gcgtccagct cgttggcgtg cagcagcgcc agcagccggg cggtcatcgc ctcgcgcaga
     2701 tgcgcctcga gcctgggata ggtggcctgg atcaccggca ggatgcgcgg caggatgtag
     2761 ggcccggcgg tcgggatgac gccgagccgc agcatgccga cgaaccccgc ggccccgcgg
     2821 gccgcgagct cgcgcatctg ccgcacctcc tcctgcacat ggcgggcgcg ggcgacgatc
     2881 gccgccccga tggccgtggt cagggcgccg cgcgggctgc gctcgaagag ggcggtgccg
     2941 agctccgcct cgagcttctt cacctgcatg ctcagggtcg gctggctcac ccggcagcgg
     3001 tcggcggcga ggccgaaatg gcccgtgtcg gccaccgcga ccaggtagtc gaggtccctg
     3061 agattcatgc gaccgatggt ctccatctat cgcgatgatc aatacaatcg attggctcga
     3121 tgggaaaccc catgctagaa gctctccagt ctggccgccg cccatgttgt cgcggccaag
     3181 ggacattgcg tccgaacgcc gccgtatcca taggcaagcg caacaacagg gcagagtggg
     3241 ggagccgatg agcgaccgac cgattctcac gacaacggcc ggcaatccga ttgccgacaa
     3301 ccagaacagc ctgaccgccg gggcgcgcgg ccccgtgctg atgcaggact accagctcat
     3361 cgagaagctg gcgcaccaga accgcgagcg aatcccggag cggccggtcc acgccaaggg
     3421 ctggggggcg cacggcacgc tgacgatcac ccacgacatc accaagtaca cgcgcgccgc
     3481 acccttcgcg aaggccggca agcagacacc gatgatcgcc cgcttctcga cggtcgccgg
     3541 cgagctgggt gccgccgacg ccgagcgcga cgtgcgcggc ttcgccctca agttctacac
     3601 cgaggagggc aactgggacc tcgtcggcaa caacacgccg gtgttcttcg tccgcgatcc
     3661 ctacaagttc cccgacttca tccacaccca gaagcggcac ccgcggacca atctccgctc
     3721 gccgacggcg atgtgggact tctggtcctt gagccccgag agcctgcatc aggtgaccat
     3781 cctgatgtcc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacttctgg
     3901 tccttgagcc ccgagagcct gcatcaggtg accatcctga tgtccgaccg cggcctgccg
     3961 accgatgtcc ggcacatcaa cggctacggc tcgcacacct acagcttcat caacgcgcag
     4021 aacgagcggt tctgggtgaa gttccatttc aagacgatgc agggccaccg gcactggacc
     4081 aatgccgagg cggccgaggt catcggccgg acccgcgaat cgacccagga ggacctctac
     4141 gccgccatcg agaagggcga cttcccgaag tggaaggtcc aggtccagat catgcccgag
     4201 accgacgccg agaagacgcc ctacaacccc ttcgatctca ccaaggtgtg gccgcacggc
     4261 gactatccgc cgatcgacgt cggcgtgctc gagctgaacc gcaacccgga caactacttc
     4321 gcggagatcg agcaggcggc gttcgcgccg tccaacatca tccccggcat cggcttctcg
     4381 ccggacaaga tgctgcaggc gcggatcttc tcctatgccg acgcgcatcg ctaccggctc
     4441 ggcacgcact acgaggcgct gccggtcaac cacgcgaaat gcccggtcca tcactaccac
     4501 aaggacgggc cgatgcggtt cttcgacaac aacacgaaga acccggatgc ctattacgag
     4561 ccgaactcgt tcaacggccc ggcccaggcg ccgagctatg ccgagccgcc gctcaggatc
     4621 tcgggcgatg ccgaccgcta cgaccaccgt gccggcaatg acgactacaa gcagccgggc
     4681 gacctcttcc ggctgatgaa cgccgagcag caggggcggc tctgccagaa catcgccgaa
     4741 gcgatgggcg gcgtgccgga gaacatcgtc cagcgccagc tcgcccactt ctacaaggcc
     4801 gacccggcct atggcgatgg cgtcgccaag gcgctcggcg tcaccttcaa gccttccacc
     4861 atggccgccg agtaactcgg ctgcggtgaa acggagaagc ccccggcacc ttcggtgccg
     4921 ggggctttct catatgtgcc gaagcccgtt cagatcgctc cctggcccat ctgctcggcg
     4981 atgtagtcgg cttgacgaat cgcgagtgcg acgatcgtca gggtcgggtt ctcggcagcg
     5041 ccggtggtga agacgctgcc gtcggagacg aaaagattgt cgatatcgtg gctctgcccg
     5101 aaggcgttga cgacgccgtc ggccggcttc tcgctcatcc ggttggtgcc gagattgtgg
     5161 gtcgaggggt agggcggcac ctcgtagatg tcggttgccc ccgccgcctc gtaaacggcc
     5221 cgcccctgcc ggaaggcgtg ctcgcgcatg gcgatgtcgt tgacgtgatc gtcgaaatgc
     5281 acgttgggga ccggcagccc atgctggtcc ttcatggtcg cgtgcagggt cacggcgttg
     5341 ctcgactgcg gcatgtcctc gccgacgatc cacagcccgg ccatgttggc gtactggtcc
     5401 atcttgcggg tgaacgatgc gccccaggca ccagggtcga ggaaggccgc catgaagggc
     5461 acgccgagcg actgcgtctc gagctcgtag ccgccggcga aaccgcgctc tggccgattt
     5521 tccgcctcgt cctggatgat cccggccatg atcgtgccac gatacatgtg caccggctgc
     5581 ccgaacgcgg catagaccga gcccgtggtg tgccgcatgt agttcctgcc gacctcgccc
     5641 gagctgttgg cgagcccgtc cgggaacatc gaggaggcgg agttcaggag cagccgcggg
     5701 ctctcgatcg agttgccggc gacgcagacg gcgcgggccg cctggcgctg ctggttgccg
     5761 tcggcgtcgg catagatcac gcccgtgacc ttgccgctcg catcgtgctc gatctgcagg
     5821 acgaacgcct ggctccgcag ctcgcagtgg ccggtatcga tggcggccgg tatctccgtg
     5881 tagagggtcg accacttggc tcccatcttg cagccctgga agcagaagcc gagctggtgg
     5941 caggcgttgc ggtcgtggcg cggctcgctg ttgatcgcca ttcgtccggt gtggaccttc
     6001 tggtagccga ggcgcgtggc gccgttccac atgaccttga agttgttgtt gccgggcagg
     6061 ccgggaatgc cgtgggtgcg ggtcacaccc atcttgtcct cggccttgtc gtagtatggc
     6121 tcgagatcgg cgagcgagag cggccagtcg agcagcgtag cgccggcgat ctcgccgtag
     6181 gtcgtcctgg ccttgaattc gtgctcacgg atgcgcagcg aggcgccggc ccagtgcgtc
     6241 gtcgttccgc ctaccgtctt gcagatccag gtcggcaggc cggcgaagtc gcgggcgacc
     6301 cgccaggtgc cggacgtggt tcgcgggtcc agccaggcga gctgcgcaaa gctcgcccat
     6361 tcgtcgttga cgaagctctc gatgctctgc tccttgccgg cctcgagcag cacgacgtcg
     6421 atgcccttct gcgcgagctc gttggccagc gtgccaccgc cggcgccgga gccgatgacg
     6481 acgacgacac tgtcgtcgga gagatcatag gtcgtcatgg ctgggctcct tctccgtacg
     6541 cgaccggggg gctggcttcg gcgtcgggct cgatcagcca gcccgcgtcc tggaagccgc
     6601 gccagacgta gccgccctgc tcgaacgacg cgccctcgta gccgaagtgc cgccagacca
     6661 gggggttgtt gtagaagtgg aagaccacgg caccccgcac cttctggaag aacgggtccg
     6721 cctgcatcag ggtcagaacc tccgtctggc tgcccggcga gagcgccgcg aagggcacgc
     6781 cgagcgccgc gtccaggcgg ccgacgccct cgctgatgag cgtggcggtc gccgggtcac
     6841 cggcggcctc ggagtccagg gcctcgacga ccaccgcata gtacatgtcg cccagcgcat
     6901 cgtgcggata gagggcccgg cacatgcgca gcagcgtctc ggccgtgggc gcgtcgagcc
     6961 gatcgagggc catcgcccag gagcgctccg cgtcggtgaa catcaccgtg cccgaggcgc
     7021 cgaacaccag catcgcctgg ccggttcgcg cgaggaaccg ccggcggtcg aggatcatct
     7081 tggtcattgg ctgcttgctc cctgatcacg aatcgttcag cggtagcgtc cgtgccgctg
     7141 caggacctcg atccgatacc cgtccgggtc ggtgaggaag aagaagcggg ccatcagccc
     7201 gccgtcgcga tcgaacgcga cgatcttgcc aggctcgagg cccaggcccg cgcagcgctc
     7261 gtgctcggca tcgagatcgt cgacgcagaa ggcgacatgg ccgtagccgt cgcccagcgc
     7321 gtagggtgcc gtccgatcct tgttcagggt cagctcgatc tcgaagtcgg cctcggcgtt
     7381 gcgcagatag acgagcgtga aggtctcgaa atcgaagcgg tcggcgaccg tcagccccaa
     7441 ggctttcccg tagaacgcga tcgagcgatc gagctcgagc acccgcacca tcatgtgaat
     7501 ggccttgggc atcttctctc ccctgtcgtt tcgagcgatt ctagagcgct cgtccgccag
     7561 agggtattat tcggctacta caagcccggc gcgcgcctcc ccgcaggcgc ccagatgcac
     7621 cgtgcacacg acccgcagga gcgacgtgaa gttgccgacg gtgccgcgca gctccagcac
     7681 ctcgtcatag agcgtggtca cgaagcgggg cgtgctcatg ccctgcccgg ccgccatggt
     7741 gtcgatgatc tcccagaacc gtgcctcgag ccggatcgag gtcacctggc catgcagtcg
     7801 gaccgagcgg gtgaccggct cgtaagcctc cggcggaaca cttgcgtaga tatggcacat
     7861 cccccgcctc ccgcagccct cggccggtgt cctaccttct tacgtccgca ggtcggcccc
     7921 tgattcctcc atgagtggcg cagagtaggc tgagactgcc gaagcatgca atcttctggg
     7981 taatcaggag gggcgccttg cccgtcggcg ccgatgtcgc catgcttccc cgcgatcatt
     8041 ggaaagtggc ggccatgtac tggagaatgc tggctggggt agcacgccgg gcgtgtcgca
     8101 atacgtgcca cctccggctt ctgccggtgc tcagcctcct ggtgctgctc ctcgtcgcgt
     8161 ctacgatgcc ggccaaggcc cagccggccg ccgacgccga cagcgccgcc atcgtcgcgg
     8221 ccctggagac gccggatggc gacctctcgc ccgaggcggc ggcagctctc gtcgctcggc
     8281 tcagcgaccg ggaggtgcgc tcgctgctgc tcgagcggct ggccgccacc ggggcggccg
     8341 gcgaagccgg cgccggcagc ccggccggga tgatcggctc gctcaccgac cggtcgtccc
     8401 tggtgcgcga gcggctgggc gagctgctcg gcgccgtcga ggacgtgccc gccgccatcg
     8461 ccaccgcctg gcaccggttc cacgacggcc ggtcgaccgg cgccatgctg gcccttgccg
     8521 ccggcgtcgc gatatccctg ctcgcggccg cgctggcgga ggccctgacc cgccgtcgcc
     8581 tggcccgcac ccgcgcccgg ctggtcggct cggaagcccg gacccaggac ctgctcaacc
     8641 gcagcctgca tctgctcggg atgctggcgc tcgatcttct cggcctcctg gtcttcgccg
     8701 tcgtcgccat ggtcgtgctg ctcgcggtca ccgaatcgca cgggccgacc cgctccgccg
     8761 tcctcggctt cctgggggcg ctcgtcgcct tccggctggt cgtgaccctc tcgcacttcc
     8821 tcctgaatcc gggcaacaag cggcaccgga tgaacgacct cgccgatgcc gacgccagct
     8881 tcctgcatcg ctggatgaag gtgatcgcgg cgatcggcac gttcggcttc gtcggctgcg
     8941 cgctggtggc gctgctcggc gagccgggcg cggagcacga tctcctgctg aacgccacgg
     9001 gcgccctcct ggtcgcctcg gtgatctatg ccgtcctcaa gggacggcgg gcgctgacca
     9061 actcgatcct cgtcttcgat gtctcgagcc cgatgcgccg ggtgctggcc gagatgacgc
     9121 cgacgctcac ggcgatcacc gtgttcctga tcttcgtgct ctacaccgtg tccagcctgc
     9181 tcggcgtcat gctgccgccg gcggccggcc tcctcaccat cgcgatcctc gtgctcgtcc
     9241 cctatgtcga tgccgtgctc gagcgcagcg ccgtctaccg ccgccgctat ggcggcgagg
     9301 ccgccgatgc cggcggcacc gtcgccacgg tgatcatccg gggtgcgcgc cttctgctct
     9361 ggatcgccag cttcctggtg ctcgccgagg tctggtcggt cgacctgcgc gctgccgcca
     9421 ccgccggcgt cggggaccgg atcagcgatg cgctggtcga tatcggcttc gcctggttcg
     9481 cggcctatct gggctgggaa ttcgcccgga tcgccatcga ccggcgcatt gccagcgaag
     9541 ccgccccgac gacaccgggc gagcacggcg acgagggcgg caccggcgcc agccgcctcg
     9601 ccacgctcct gccgctggcc aagcgcgcca tccagatcac catcggcgtg atcaccgtga
     9661 tgatcacgct ctccgcgctc ggcgtcgata tcgggccctt gctcgccggc gccggcgtgg
     9721 tcggcatcgc ggtcggcttc ggcgcccaga cgctggtgcg cgacgtggtc tccggcgcct
     9781 tctttctgct cgatgacgcg tttcgggtcg gcgagtatgt cgacgtcggc agcgtcaagg
     9841 gcacggtcga gaagatctcg gtccgctcct tccgcctgcg ccaccatcgg ggtgccctgc
     9901 atacggtgcc gttcggcgag atccgccagc tcaccaacta cagccgcgac tgggtcatca
     9961 tgaagctgga gttccgggta ccgttcgaca ccgacccgga tcgggtgcgc aagatcttca
    10021 agcagatcgg ccaggacctg ctcgccaacc cggagctcgg ccccgatttc atcgagccgt
    10081 tcaagtcgca gggcgtcttc acgctcgacg acagcgcgct cgtcatccgc ggcaagttca
    10141 tggccaagcc gggcaagcag ttcgtcgccc ggcgcgagat ctacaacgcc gtgcagcgcg
    10201 ccttcgccgc cgccggcatc cgcttcgccg atcgccgggt cacggtgcag gtggccgacg
    10261 gcgaggggat gacgacggag gagcgccaac gcgtcgccca ggcggccgcc agcgccctgc
    10321 agccacccgc cgccgccgcg gcgagcggct cctgaacctc agccgcgccc cgcgctcggc
    10381 acgctgccgg cccgacgcac gccccggctg accggcgtgg gaccgcgcga ccccaaaccg
    10441 ctcgatgtgg atccgccctc catcgccagc agccggcgca cccgctcttc ggtcgcggga
    10501 tgggtgcgga agaggttgtc gatgccgccc ttgatcagcg ggttgatgat gaacatgtgg
    10561 gcactcgccg ggttgtgctc ggccgtctgc atgacgcggc cccgggcgag ctgctcgatc
    10621 ttcaaaagtg ccccggcgag ccagcgggga tggccgcaga tcgccgcgcc gtcgcggtcg
    10681 gcggagtact cccgcgtgcg cgatatcgcc atctgcacga ggccggccgc catgggggcg
    10741 aggatcatga tgaggatcat cgcgatcggg ttgggccggt tctcgccccg cccggcgccg
    10801 aacagcatgg cgaagttggc gaggaagccg atggccccgg caatggtcgc ggccaccgtc
    10861 atggtcagcg tgtcgcggtt cttgatgtgc gccagctcgt gcgccatcac cccggccagc
    10921 tcctcgcggc tcaggctgcg cagcaggccg gtggtcgccg ccacggccgc atggtccggg
    10981 ctccgcccgg tggcgaaggc attgggctgg tcggtctcga tcacgtagac ctgcggcatc
    11041 ggcagccccg cccgcttcgc caggccttcg accacaccgt agtactccgg tgcggaggcg
    11101 cgcgtcacga cccgcgcatt gtgcatgcgc agcaccatgc tggccgagtt ccagtaggcg
    11161 aagaagttca tccccagcgc cagcagcagg gcgatcagga gcccgccctc gccgccgagg
    11221 acgaagccca cggcgaggaa gagcccggtc atcgccgcga gcaggatgcc ggtgcgcaat
    11281 gcgttcatcg tcggtcgtcc gagccagtcc caaccgtcct tgaaatggga ccggcggcgc
    11341 gccctgcaag catgccccgt gctgcggcac ttgtgagacg tgacccaagc gcgttatctg
    11401 gcatgcggtg agcgtcctgc cggcggccgg acgagcaacc caaacggggg aaggcgaccg
    11461 acatgacatc gacgagccag ccgcagcggc atgcttcgac accggccgac gacctgctcg
    11521 gcgacgacga cctcatggat gccgacgagc cggtgatccc gcgcagcatc gtcgattcgc
    11581 acgagccggt gaccagcgag gaggcgctcg agctgcaccg gcgcggccgg cccggcaaga
    11641 tcgaggtcac gccgaccaag ccgctcgcca cctcgcgcga tctctccctc gcctacagcc
    11701 cgggcgtcgc ggcaccctgc ctcgccatcc aggccgaccc ggttcgggcc tacgagtaca
    11761 ccgccaaggg caacctcgtg gcggtcatct cgaacggcac ggcggtgctc ggtctcggca
    11821 atctgggggc cttgggctcg aagccggtga tggagggcaa ggccgtcctc ttcaagcgtt
    11881 tcgccgacat cgacggcatc gacctcgagg tcgacaccga ggaggtcgac gccttcgtcg
    11941 actgcgtcaa gctgctcggg cccaccttcg gcggcatcaa cctcgaggac atcaaggcgc
    12001 cggagtgctt cgtcatcgag cagcgcctgc gcgagctcat ggatatcccg gtcttccacg
    12061 acgaccagca cggcaccgcg atcatcgccg ccgccgggct gatcaacgcg ctcgatctca
    12121 ccggccgctc gatcgccacc gcccgcatgg tggtcaatgg ggccggctcc gccggcatcg
    12181 cctgcgccga gctgatcaag gcgatgggca tgccgcacga gaacgtcacc atctgcgaca
    12241 ccaagggggt gatctaccag ggtcgtaccg aaggcatgaa ccagtggaag tcggcgcacg
    12301 cggtgccgac cgaggcccgc accctcgccg acgccatggc aggcgccgac gtcgtcatgg
    12361 gcctctcggc caagggtgcc ttcacgaacg agatgatcgc ctcgatggcg ccgaacccga
    12421 tcatcttcgc catggccaac ccggaccccg agatcacgcc cgaggacgcg aaggccatcc
    12481 gcgacgacgt gatcatcgcg acgggccgca gcgactaccc caaccaggtg aacaacgtcc
    12541 tgggcttccc ctacatcttc aggggcgctc tcgacgtccg ggcctcgacc atcaacgacg
    12601 ccatgaagat cgccgccgcc tacgccatcg ccgccctcgc ccgcgaggag gtgccggagg
    12661 aggtgaccgc cgcctatcgc ggccggcggc tgcgatatgg cccggactac atgatcccga
    12721 ccccgttcga tccgaggctg atcacccacg tgccggcggc ggtcgccaag gcggcggtcg
    12781 agagcggcgt cgccatgaag ccgatcgtcg atttcgagcg ctaccgccac gagctgcgct
    12841 cgcgcctcga cccgacggcg agccgcatgc agctcatcat cgaccgggtg cgtgcccacc
    12901 ccaagcgcat cgtcttcgcc gagggcgagg aggaaaaggc gatcagcgcc gccctcgcct
    12961 ggcgcaatgg cggcctcggc ctgccgatcc tggtcggccg cgaggagcgg atcagggaca
    13021 ccgccaaggg catgggccag agcagcctcg acggcatcga gatccacaat gcccggctca
    13081 gcccgcacaa ccgcctctat gcggatttcc tctacgagcg caaccagcgc aacggcctcc
    13141 tgttccgcga ctgccagcgg ctggtcaacc aggaccgcaa cgtcttcggc gcctgcctcg
    13201 tcgcctgcgg ccatgccgac gccatggtga cgggcctgac ccgccgctac aacacggtga
    13261 tcaaggacat ccgcctcgtc atcaacccgc tgccgaggaa gcggatgttc ggcgtgacca
    13321 tcatcatcgc ccgggggcgc accgtcttca tcgccgacac cacggtgcac gagctgcccg
    13381 atcccgaggt ctgcgccgac atcgccatcc agacggccga ggtggcggag aagatgggct
    13441 tcacgcccag ggtcgccttc ctctccttcg ccaccttcgg caacccccgg atcagcaagg
    13501 cggccagggt gcgcgactcg gtccgcgccc tcgacgagcg gcaggtcgac ttcgagtatg
    13561 acggcgagat gaccgccgac gtcgccctcg atccggagct catggcgctc taccccttct
    13621 gccgcctcag cggccccgcc aacgtgctga tcatgccggc cctgcacacc gcccacatct
    13681 cggcgaagct cctgcagcag ctcggcggcg gcacggtgat cggcccgctc ctgatcggcc
    13741 tcgagaagcc cgtgcagatc gtgccgatga acgccagcgt ctccgacatc ctcaacgtcg
    13801 ccgcgctcgc cgcccacgat gcccaggcga tcgaggcgga agcggccgcc ggctgagcgc
    13861 ccgccatgcc gaacatcgtc gccgtcacgc ccggcttcca ccgcatcccc ctgccggtgg
    13921 tgctgtccga cagcacgcat gggctgatga gcgccttcga gctgatcact gtccgcatca
    13981 ccgacgccga aggggcggag ggggtgggct acacctacac ggtcggccgc aatggcgggg
    14041 cgatcatgga cacgctcgcc cgcgagatcg aggagctcgt aatcggcgac gacgccgacc
    14101 gcatcgaggc gctctggcag aagctctggt gggcgctgca ctatggcgga agggggggac
    14161 ccaccgtcct cgccctctcg gccttcgaca tggcgctctg ggacctgaag gcccggcgcg
    14221 ccggcctgcc cttgtggaag ctgctcggcg gccacgaccc gcgcgtcacc tgctatgccg
    14281 gcggcatcga tctcgacctc ccgctcgacg ccctcctggc ccagaccgac ggcaatctca
    14341 gccgtggctt tcgcgccatc aagatgaagg tcggccggcc gaagctctcg gaggacacgg
    14401 cccgcatcgc cgccatgcgc gagcatctgg gcgacggctt cccgctcatg gtcgacgcca
    14461 acatgaaatg gaccgccgac caggcgatcc gcgccgcccg cgccttcc
//
LOCUS       DAOTFI010000122        14447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5026_length_14447_cov_7.353252, whole genome shotgun sequence.
ACCESSION   DAOTFI010000122 DAOTFI010000000
VERSION     DAOTFI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14447
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5026_length_14447_cov_7.353252"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..677
                     /locus_tag="P5558_13415"
     CDS             87..677
                     /locus_tag="P5558_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25356.1"
                     /translation="MRYRLATALALGLLAACTTPEPPPPPPPPPTPAPPPVMEPPPPV
                     DPATRVDRLCRPLLGIVDAEPAGFSSLRGAPAGPRAWQGRAVPDGFYTCEIDGPTNPG
                     AQYVCRGNRVAGGGAKLLEDDFDRIASDLDACLARSEWYPRNWQRGEVMLFAGGERQL
                     VWRDLAPNPRPAIALKIEEEIGTRVYFLRLAVFTLF"
     gene            complement(696..1115)
                     /locus_tag="P5558_13420"
     CDS             complement(696..1115)
                     /locus_tag="P5558_13420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HRY25357.1"
                     /translation="MTSDPTELADRFADAWNSHDMGALAGLFEDAARFVNVVGMFWRS
                     REEIRQAHAFSHESMFRNSTLAVKAVENTALAPGVVAVHVHWHLEGHLGPTGEPEQPR
                     DGVLLLVARETRQNGGWQIAVAQNTDAIPGVVTIPQR"
     regulatory      1289..1387
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1554..2648
                     /gene="gcvT"
                     /locus_tag="P5558_13425"
     CDS             1554..2648
                     /gene="gcvT"
                     /locus_tag="P5558_13425"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRY25358.1"
                     /translation="MPDPQTTPLDALHRELGARMVDFAGWSMPVQYPTGILKEHVWTR
                     TRASLFDVSHMGQIRMPGADAAAAIESLVPADIQALKPGRARYTQFTTLDGGIIDDLI
                     VTATDDGLYLVVNAGGRDTDLAHLREHLPGREIELLADRALLALQGPAAVNVLARLAP
                     EVAGLRFMSSIELEAAGLELRISRLGYTGEDGFEVSVAAGHAVELARTLLAAEEVQPA
                     GLGARDSLRLEAGLCLYGHDIDRTTSPIEADLAWTVQKRRRAEGGFPGAERILAELRD
                     GPARRLVGIRPEGRAPAREGAAILDGSGQAIGKVTSGGFGPTVDGPVAMGYVAGAAAE
                     PGTPLQLEVRGRKLPARVEALPFVPHRYHR"
     gene            2663..3037
                     /gene="gcvH"
                     /locus_tag="P5558_13430"
     CDS             2663..3037
                     /gene="gcvH"
                     /locus_tag="P5558_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRY25359.1"
                     /translation="MARYYTKEHEWLDVTGDVATVGITDHAQEQLGDVVFVELPKAGT
                     TVAKGAEVAVVESVKAASDIYAPASGKVVEANTALEDTPAGVNEDAEGAAWFFKLELS
                     DKSELDGLMDADAYAAFLETIA"
     gene            3057..5921
                     /gene="gcvP"
                     /locus_tag="P5558_13435"
     CDS             3057..5921
                     /gene="gcvP"
                     /locus_tag="P5558_13435"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRY25360.1"
                     /translation="MADIENSATVDPVAAPFVRRHVGPDTAEIAAMLQTVGAASLDDL
                     IGETVPRKIRTTDPLDLPPALTEHAALARLADLAASNDVRRSLIGMGYHGTHTPPVLL
                     RNLFENPGWYTAYTPYQAEVSQGRLEALLNWQQAVVDLTGMELANASLLDEATAAAEA
                     MTLAHRSTRGKGDRFFVDADSHPQTLAVLRTRAEPLGIEIVVGDPLADLDAGSVYGAL
                     LAYPGSSGEIRDFRPAVEALHGAGAIVAVASDLLALCLLEAPGHWGADIVLGSAQRFG
                     VPMGYGGPHAAFFATKDALKRSVPGRIIGVSQDARGKPALRMALQTREQHIRREKATS
                     NICTAQVLLAVMAGMYAVWHGPRGLKRIASRVHDLTLDLAARLDAAGIERTNRHFFDT
                     LTLEVPGKARKLLAEAEKAGFNLRAIDADHLALSLDETVTAEEVEKLADILGGKASNA
                     TGPGLPEKLARQTPYLGHPVFSAHRSETQMLRYLRKLQLKDLALDRSMIPLGSCTMKL
                     NAAAEMIPVSYPGFADLHPFAPKGQAEGYARLFADLEAWLATITGFDAVSLQPNAGSQ
                     GEYAGLLVIRAFHRAQGQGHRDVCLIPSSAHGTNPASAVMAGMTVVVVACDDQGNVDL
                     ADLEAKAGKHEDRLAALMITYPSTHGVFEERVKDVCATVHAHGGQVYLDGANLNALVG
                     LVRVAELGADVSHMNLHKTFCIPHGGGGPGMGPIGVKAHLAPYLPGHPVTGEGGKETL
                     GTVSAAAFGSPSILPITWAYIAMMGAAGLRHATEVAILNANYIVARLKDHFPIVYAGR
                     NGRVAHECIIDFRDIKQETGITAEDVAKRLVDYGFHAPTMSWPVAETMMIEPTESEAR
                     AEIDRFCDAMIQIRGEIRQIETGEADRADNPLKRAPHTADLLMADWTRPYSRETAFTP
                     LGGDPADKYWPPVARVDNVYGDRHLVCTCPPIEAYAEAAE"
     gene            complement(5936..6274)
                     /locus_tag="P5558_13440"
     CDS             complement(5936..6274)
                     /locus_tag="P5558_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25361.1"
                     /translation="MLQRFLPAVPLALVLLAVGATPAGAVRARGTFDQCVVTWGSFCP
                     VETIGLSLLGSAALAAFGLAIWLVLAKLRDRAGRSGLGGVAALLGLAARYPIHTFAVV
                     VLGVAALFGL"
     gene            complement(6404..7639)
                     /locus_tag="P5558_13445"
     CDS             complement(6404..7639)
                     /locus_tag="P5558_13445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY25362.1"
                     /translation="MRSRLAFSLFWKIYLTLVACLAMVALAMGALVHLAGGDDFEGWP
                     DRGARFFAAMLPPGSGDAEVERLAAALGAELALRGADGALLAGSADLRAGWRPRVMDF
                     ALDDGRAVLVRLDGPWRRRDGGPFLAVIVVASTLALGAYPVVRHLTRRLERLRQGVEA
                     WGEGALETRVGLAGRDEVAAVARSFDRAAETVERLIGAHRALLANASHELRSPLARLR
                     LAIDMGDGGTAEAARREIVQSLAELEALVEEILLTSRLDHAALPERAEPVDLLALAAE
                     EAARVEAAVEGTPVTVVGEERLLRRLVRNLLQNAVRHGRPPVEVTVRPTADGGARLAV
                     RDHGPGVPAGEAERVFEPFYRPKGHGEDAGGWGLGLALVRQIARRHGGDVRHETPEGG
                     GAVFVVELPGGAEGRVKHG"
     gene            complement(7657..8349)
                     /locus_tag="P5558_13450"
     CDS             complement(7657..8349)
                     /locus_tag="P5558_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY25363.1"
                     /translation="MAGEVLIVDDDLRLAAMLRDYLTRAGYAVRTAATGSAGLAELAR
                     RAPDVVVLDVMLPDLDGFEVCRRLRRVSTVPVLMLTARGDETDRIVGLELGADDYLPK
                     PFNPRELLARLRAILRRGRGEAEASLRFGRLEVDPGSRRARVDGEERSLTSYQLDLLL
                     ALARHPGRVLSRDQLMELVKGTDGEAFDRSIDVHISRIRAAIEDDPKRPRRILTVRGA
                     GYVFARAQDDEA"
     gene            complement(8376..8909)
                     /locus_tag="P5558_13455"
     CDS             complement(8376..8909)
                     /locus_tag="P5558_13455"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HRY25364.1"
                     /translation="MTDGKDTNGTGQAGGGQHRAPTRWGHRILIGGAVLVVAVGVGAG
                     VVAATGSGWHGGWHHGGMRDFAEWRLTRALDAVDARPEQQARITAIAEATHDELAPMM
                     EALRGARGELAALLVEPVIDKAAVEELRSSRMAMLDDASKRAVAALLEAAEVLDPAQR
                     AALAARLEERRGRRGLD"
     gene            9188..10102
                     /locus_tag="P5558_13460"
     CDS             9188..10102
                     /locus_tag="P5558_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY25365.1"
                     /translation="MDIYLPVAEMSVNLFLILGLGGMVGFLSGVFGVGGGFIMTPLLI
                     FIGIPPAVAVGSGASQILASSTSGAIAHTRRRTVDFRMGWVLIAGGAVGSGLGILLFR
                     WLRATGQIDVVIALAYVLFLGVIGALMFAESARTLFRTRKSSPATRRRKLHQHHWLHG
                     LPMKMRFNQSRLYISVLTPLAVGAGVGVLVAIMGVGGGFIMVPAMIYLIGMPTSVVIG
                     TSLFQIAFVTAITSFLHAYTNQAVDIVLALLLIAGGVIGVQFGSRAGAVLKGEQLRIL
                     LAILVLAVALKMAWDLVATPADVFSIAR"
     gene            10110..10592
                     /gene="ybaK"
                     /locus_tag="P5558_13465"
     CDS             10110..10592
                     /gene="ybaK"
                     /locus_tag="P5558_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRY25366.1"
                     /translation="MAKGTPAVQALTRAKIAFTLHEYDFVATPGAIGLQAALAMGVAP
                     ERVQKTLMAVVDEQELVVAVIAVDRELDLKALAKAAGGKRAAMAEVARAERATGYVKG
                     GISPFGQKKRHRTFLDATALALPTIIVNGGRRGLQIELAPADLLAATAGTATALARVI
                     "
     gene            10668..12590
                     /locus_tag="P5558_13470"
     CDS             10668..12590
                     /locus_tag="P5558_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRY25367.1"
                     /translation="MSDTKLALAADFPPADPAAWRELVEKTLKGADFAATLVSRTDDG
                     IEVQPLYTTSAARPGLPGSPPHLRGATAAGHVAGGWDIRQLHDHPDPALANRAILEDL
                     EQGVTSIVLRLDAAFAQGEDEPDGILARNADGLDRVLDGVMLDLAPVQLMAPGHTVPA
                     ARALLELLRRRDHAPDTVTADLGLDPMGAVALGRADPTDALPPAFDLVRQVAGEWRKL
                     DLLRVDGELYHAAGASEAQELAAAIATGIQYLREGEAVGLEPASLARRLGFTLAADAD
                     LFLTIAKCRAARRLWPRVMTACGADDVPMRLDLRTASRMLTRRDPWVNMLRTTVACLA
                     GVAGGAEAITVAPFDAALGEPGRLGRRIARNVQLVLMEECNLHRVIDPAGGSPYVETL
                     SLALAERAWPLVQAIEGEGGMLASLRQGIVQDSIAATCLARLDDLAHRRRAITGVSSF
                     PDLDEQTLPPAEPDLGPLLEQAAASLPPRTKPAATVAALTPMRLAEGFERLRDLGDLA
                     LIEHGQRPRVFVAAMGPLARHGAEASFVRNALAAGGIEAIVSPPLEDSDAAASAFAES
                     GTTIACLCGIDRTKPATAGPIARALLDGGAIAVYAAGRPDPALRAEGIDGFVHTGANI
                     LGLLEDLHSLALGEAA"
     gene            12587..>14447
                     /gene="scpA"
                     /locus_tag="P5558_13475"
     CDS             12587..>14447
                     /gene="scpA"
                     /locus_tag="P5558_13475"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRY25368.1"
                     /translation="MTRVPDFTRLPLDDGPVTARGDWASAFLAETGQTPEALAWATPE
                     EIDVRPLYGPDDLAQVDHLDGLPGLPPFVRGPYPTMYVGQPWTIRQYAGFSTAEESNA
                     FYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVAGDVGMAGVAIDSILDMRQLFHGIPL
                     DRMSVSMTMNGAVLPVLALYILAGEEQGVAPEQLAGTIQNDILKEFMVRNTYIFPPKP
                     SMRIISDIFRFTAERMPKFNSISISGYHMQEAGATADLELAYTLADGIEYLRTGEAAG
                     LDIDRFAPRLSFFWAIGMGYFMEVAKLRAARLLWAKLVRPFEPRDPRSLSLRTHCQTS
                     GWSLTAQDVMNNVVRTCVEALAATHGGTQSLHTNAFDEALALPSEFSARIARNTQLFI
                     QDETGIRRTVDPWGGSFFVERLTHDLAARAWGHIEEVEQAGGMAKAIEQGIPKLRIEE
                     AAARTQARIDSGRQTVVGVNKYRPGEPEHVDVLKVDNRAVRASQIAKLEQLRKGRDEP
                     ALRRALDALTRSAGTGEGNLLALAVDAARASATVGEITSALEQVWGRHVAEIRSITGV
                     YGREVGAMDSVRACRERVRAFAEAEGRRPRILIAKMGQDGHDRGQKVIASAFAD"
BASE COUNT         2099 a         5761 c         4626 g         1961 t
ORIGIN      
        1 ggccagatcg cgctgccgga caagcactcg gtgacgatcg ccgatctcgc cagcctgcgc
       61 gccgcgtcgg gcgactgaga gccgacttga gataccgcct cgccacggcg ctcgccctcg
      121 gcctgctcgc cgcctgcacc acgcccgagc cgccgccacc gccgccacct cccccgacac
      181 ccgccccgcc gccggtcatg gagccgccac cgccggtcga tccggccacc cgggtcgacc
      241 gcctctgccg ccccctgctg ggcatcgtcg acgccgagcc cgccggcttc tccagcttgc
      301 gcggcgcgcc ggccgggccg cgcgcgtggc agggccgcgc cgtgcccgac ggcttctata
      361 cctgcgagat cgacggcccg accaaccccg gcgcgcaata tgtctgccgc ggcaatcgcg
      421 tcgccggtgg cggggcgaag ctcctcgagg acgacttcga ccgcatcgcc agcgatctcg
      481 acgcctgcct cgcccgcagc gaatggtacc cccgcaactg gcagcgcggc gaggtgatgc
      541 tgttcgccgg cggcgagcgc cagctcgtct ggcgcgatct cgccccgaac ccacgcccgg
      601 ccatcgccct caagatcgag gaggagatcg gcacccgcgt ctacttcctc aggctcgccg
      661 tcttcaccct cttctgagcg ggcggctttc gtcgattacc tctgcgggat cgtcacgacg
      721 ccgggaatgg cgtcggtgtt ctgcgccacg gcgatctgcc agccgccatt ctgccgggtc
      781 tcgcgggcga ccagcagcag cacgccgtcg cgcggctgct ccggctcgcc tgtggggccc
      841 agatggccct cgagatgcca gtggacatgc accgcgacga cgcccggtgc cagcgccgtg
      901 ttctcgacgg ccttcacagc gagcgtcgaa ttcctgaaca tgctctcgtg gctgaaggca
      961 tgtgcctgcc ggatctcctc ccggctgcgc cagaacatgc cgacgacatt gacgaagcgc
     1021 gccgcgtcct cgaacagccc tgcaagggcg cccatgtcgt ggctattcca ggcgtcggcg
     1081 aagcgatcgg caagctcggt ggggtcggat gtcatggtcg tgtcttggcg gcatcgaggc
     1141 atcgccgagc cggaattggc tcgaggcggc tcggccagga caacacgatg cgccgactgg
     1201 aggctcctgg caactgcccg ggcttcggcg ccgtggcgat caccccgctt gacccggcac
     1261 ctgcctccgc taccatccgt tccggctgca atcgcccgtg ggagagtccg tctcggccat
     1321 tggccgggcc ggcgccgaag gagcaaccgc cccggaaact ctcaggcccc aggaccgcga
     1381 gcgagttggc cctctggaga gcggatgcct cggcatccca ccgaagggga agacgggagg
     1441 ccccggccgc ccgtctgaat ctctcaggtc caagacagag ggggcgtcga gacgggcgaa
     1501 tcacgcccat tgctcggcgt ccgctgtcga aacctgaacg aggtccagag cccgtgcccg
     1561 atccgcaaac cacgccgctc gatgcgctcc atcgcgagct cggggcccgc atggtcgact
     1621 tcgccggctg gtcgatgccg gtccagtacc cgaccggcat cctcaaggag cacgtctgga
     1681 cgcgcacccg ggcctcgctc ttcgatgtct cgcacatggg ccagatcagg atgcccggcg
     1741 ccgatgccgc ggccgccatc gaaagcctcg tgccggccga catccaggcc ctgaagcccg
     1801 gccgcgcccg ctacacccaa ttcaccacgc tggacggcgg catcatcgac gatctgatcg
     1861 tcaccgccac cgatgacggg ctctacctcg tcgtcaatgc cgggggccgg gacacggacc
     1921 tcgcccatct tcgcgagcat ctgccgggcc gcgagatcga gctcctcgcc gaccgtgccc
     1981 ttctcgccct gcagggcccg gccgccgtca acgtgctcgc gaggctcgcc cccgaggtcg
     2041 ccggcctccg cttcatgtcc agcatcgagc tcgaggccgc cggcctcgag ctgcgcatct
     2101 cccgcttggg atacaccggc gaggacggct tcgaggtctc ggtcgcggcc ggccacgcgg
     2161 tcgagctcgc ccgcacgctg ctcgccgccg aggaggtcca gccggccggc ctgggggcgc
     2221 gcgattccct ccgcctcgag gcgggcttgt gcctctacgg ccacgacatc gaccggacga
     2281 ccagcccgat cgaggccgac ctcgcctgga cggtccagaa gcggcgccgg gccgagggcg
     2341 gcttccccgg tgcggagcgc atcctggccg agctcaggga cggccccgcc cgccgcctcg
     2401 tcggcatccg ccccgaaggc cgtgcccccg cccgcgaggg tgccgccatc ctggacggtt
     2461 ccggccaggc gatcggcaag gtcaccagcg gcggcttcgg cccgaccgtc gatggccccg
     2521 tcgccatggg ctacgtcgcc ggcgctgccg ccgagcccgg cacacccttg cagctcgagg
     2581 tgcgcggcag gaagctgccg gcccgcgtcg aggcgcttcc cttcgtcccc caccgctacc
     2641 accgctgaag gagcttcagc ccatggcccg ctactacacc aaggagcacg agtggctcga
     2701 cgtcaccggc gacgtcgcca cggtcggcat caccgaccac gcccaggagc agctcggcga
     2761 cgtcgtcttc gtcgagctgc cgaaggccgg caccaccgtc gccaagggtg cggaggtcgc
     2821 cgtggtcgag agcgtcaagg cggcgagcga catctacgcc cccgcctccg gcaaggtggt
     2881 cgaggccaac accgccctcg aggacacccc ggccggggtc aacgaggatg ccgagggtgc
     2941 cgcctggttc ttcaagctcg agctttccga caagagcgag ctcgacggcc tgatggacgc
     3001 cgacgcctac gccgccttcc tcgaaaccat cgcctgaccg cccggagcac ccgtccatgg
     3061 ccgacatcga gaacagcgcc accgtcgatc cggtggccgc gcccttcgtc cgccggcatg
     3121 tcggccccga cacagccgag atcgcggcca tgctgcagac cgtcggcgcc gcctcgctcg
     3181 acgatctgat cggcgagacg gtgccccgga agatccgcac cacggacccg ctcgacctgc
     3241 cgcctgccct gaccgagcac gccgccctcg cccgcctcgc cgacctcgcc gccagcaacg
     3301 atgtccgccg ctcgctcatc ggcatgggct accacggcac ccacacgccg ccggtcctgc
     3361 tcaggaacct cttcgagaac ccgggctggt acaccgccta cacgccctac caggccgagg
     3421 tcagccaggg ccggctcgaa gcactcctca actggcagca ggccgtcgtc gacctcaccg
     3481 gcatggagct ggccaacgcg tcgctgctcg acgaggcgac cgccgccgcc gaggccatga
     3541 cgctcgccca ccgctcgacc cggggcaagg gcgaccgctt cttcgtcgac gccgacagcc
     3601 atccccagac cctggcggtg ctgcgcaccc gcgccgagcc gctcggcatc gagatcgtcg
     3661 tcggcgaccc gctcgccgac ctcgacgccg gcagcgtcta tggtgccctc ctcgcctatc
     3721 cgggctcgtc gggcgagatc cgcgacttcc gccccgccgt cgaggcgctg catggcgccg
     3781 gcgccatcgt cgccgtcgcc agcgacctcc tcgccctctg cctcctcgag gcacccggtc
     3841 actggggggc cgacatcgtc ctcggatccg cccagcgctt cggcgtgccg atgggctatg
     3901 gcggcccgca cgccgccttc ttcgccacca aggacgccct caagcgctcg gtccccggcc
     3961 ggatcatcgg cgtcagccag gacgcgcgcg gcaagccggc gctccgcatg gcgctgcaga
     4021 cccgcgagca gcacatccgc cgcgagaagg cgaccagcaa catctgcacg gcccaggtcc
     4081 tgctcgccgt catggccggc atgtacgccg tctggcacgg gccaaggggc ctgaagcgga
     4141 tcgcgagccg cgtccacgac ctgaccctcg acctcgccgc ccggctcgac gccgccggca
     4201 tcgagcgcac caaccgccac ttcttcgaca ccctcaccct cgaggtcccg ggcaaggcca
     4261 ggaagctcct ggccgaggcc gaaaaggccg gcttcaatct gcgcgccatc gacgccgacc
     4321 acctcgccct ctcgctcgac gagaccgtca ccgccgaaga ggtcgagaag ctcgcggaca
     4381 tcctcggcgg taaggcctcg aatgcgaccg gaccgggcct gccagagaag ctggcccgcc
     4441 agacccccta cctcggccac ccggtcttca gcgcgcatcg cagcgagacc cagatgctgc
     4501 gctacttgcg caagctccag ctcaaggacc tcgccctcga ccgctcgatg atccccttgg
     4561 gctcctgcac catgaagctc aacgccgcgg ccgagatgat cccggtgagc tatccgggct
     4621 tcgccgatct ccaccccttc gcgcccaagg gccaggcaga gggctatgcc cggctcttcg
     4681 ccgatctcga ggcctggctc gccaccatca ccggcttcga tgccgtctcg ctccagccca
     4741 atgccggcag ccagggcgag tatgccgggc tcctcgtcat ccgcgccttc caccgggccc
     4801 agggccaggg tcaccgcgac gtctgcctga tcccttcctc ggcgcacggc accaacccgg
     4861 ccagcgccgt catggccggc atgaccgtgg tggtcgttgc ttgtgatgat caaggcaatg
     4921 tcgacctcgc cgacctcgag gccaaggccg ggaagcacga ggaccgtctc gccgccctga
     4981 tgatcaccta cccatcgacc cacggcgtct tcgaggagcg ggtcaaggac gtctgcgcca
     5041 cggtccacgc ccatggcggc caggtctatc tcgacggcgc caacctgaac gcgctcgtcg
     5101 gcctcgtccg cgtggccgag ctcggcgccg atgtcagcca catgaacctg cacaagacct
     5161 tctgcatacc gcatggcggc ggcggccccg gcatggggcc gatcggcgtc aaggcccacc
     5221 tcgcccccta cctcccgggc caccccgtga ccggcgaggg cggcaaggaa accctcggca
     5281 cggtctccgc cgccgccttc ggctcaccct ccatcctgcc catcacctgg gcctacatcg
     5341 ccatgatggg ggcggccggg ctgcgccacg ccaccgaggt cgcgatcctc aacgccaact
     5401 acatcgtggc ccggctcaag gaccacttcc ccatcgtcta tgccggccgc aacggccgcg
     5461 tcgcgcacga gtgcatcatc gacttccgcg acatcaagca ggaaaccggc atcaccgccg
     5521 aggatgtcgc caagcgcctc gtcgactacg gcttccacgc gcccaccatg tcctggcccg
     5581 tggccgagac gatgatgatc gagccgaccg aaagcgaggc cagggccgag atcgaccgct
     5641 tctgcgacgc catgatccag atccgcgggg agatccgcca gatcgagacc ggcgaggccg
     5701 accgtgccga caaccccctg aagcgcgccc cgcacaccgc cgacctcctg atggccgact
     5761 ggacccgccc ctacagccgc gagaccgcct tcacgccgct cggcggcgat cccgccgaca
     5821 agtactggcc ccccgtcgcc cgcgtcgaca acgtctacgg cgaccgccat ctggtctgca
     5881 cctgcccgcc gatcgaagcc tacgccgagg cggccgagta gggggtcgaa ggccgtcaga
     5941 ggccgaaaag cgccgccacg ccgaggacga cgacggcgaa ggtgtggatg ggatagcgcg
     6001 ccgcgaggcc gagaagggcc gcgaccccgc cgaggcccga tcgcccggcg cggtcgcgca
     6061 gcttcgccag cacgagccag atggcgagcc cgaaggcggc cagcgccgcc gagccgagca
     6121 gggaaagccc gatcgtctcc accgggcaga acgagcccca ggtgaccacg cactgatcga
     6181 aggtgccgcg cgccctgacc gcgccagcgg gcgtcgcgcc gacagccagg agaacgagcg
     6241 ccagcggcac ggccggcagg aaacgctgga gcaaggggcg gtccctccac tgagcgggcc
     6301 cggacctcgg cgcctgctcg gtgcactctc cccgatcggc aggatgtccg cttccagcga
     6361 gcgtcaggaa gcgcgcgtcg gtaccgccgt ttctgccaca gggttaacca tgcttaaccc
     6421 tgccctcggc cccgcccggc agctccacca cgaacacggc accgccgccc tcgggcgtct
     6481 cgtgcctgac atcgccgccg tgccggcggg cgatctggcg gaccagcgcc aggccgaggc
     6541 cccagccgcc ggcgtcctcg ccgtggccct tcggccggta gaagggctcg aagacccgct
     6601 ccgcctcgcc cgccggcacg cccgggccat ggtcgcgaac ggcgagccgc gcgccgccgt
     6661 cggccgtggg ccggaccgtg acctcgaccg gggggcgccc gtgtcgtacc gcgttctgca
     6721 ggaggttccg caccagccgg cgcaggagcc gctcctcgcc cacaacggtg accggcgtcc
     6781 cctcgaccgc cgcctcgacc ctggccgcct cctcggccgc cagcgccagt aggtcgaccg
     6841 gctcggcccg ctccggcagc gccgcgtggt cgagccggct ggtcagcagg atctcctcga
     6901 ccagcgcctc gagctcggcg aggctctgga cgatctcccg ccttgccgcc tcggctgtgc
     6961 caccatcgcc catgtcgatc gccagccgca gccgggccag cggcgagcgc agctcgtggc
     7021 tggcattggc gagcagggcg cggtgcgcgc cgatcagccg ctcgaccgtc tcggccgccc
     7081 ggtcgaagct cctcgccacc gccgccacct cgtcccggcc ggcgaggccg acgcgggtct
     7141 ccagcgcccc ctcgccccag gcctcgacgc cctgccgcag ccgctcgagc cgccgcgtca
     7201 ggtggcggac caccggatag gcgccgagcg ccagggtcga tgccacgacg atgacggcga
     7261 ggaagggtcc gccatcgcgc cgccgccagg ggccgtcgag ccgcaccagc acggcccggc
     7321 catcgtccag cgcgaagtcc atcacccgcg gccgccagcc ggcgcggagg tccgcagagc
     7381 ccgccaggag cgcgccatcg gcgccgcgca gcgccagctc ggcgccgagc gccgccgcca
     7441 gacgctcgac ctcggcatcg ccgctgcccg gcggcagcat ggccgcgaag aaccgggcac
     7501 cgcgatccgg ccagccttcg aaatcgtcgc cgccggcgag gtgcacgaga gcccccatcg
     7561 ccagcgccac catggcgagg caggcgacca gcgtcaggta gatcttccag aagaggctga
     7621 aggccagcct ggaccgcaac ctgtgtccca gccccctcaa gcctcgtcgt cctgcgctct
     7681 tgcaaagaca tagccggcgc cgcgcaccgt caggatgcgc cggggccgct tcgggtcgtc
     7741 ctcgatggcg gcccggatgc gcgagatgtg gacgtcgatc gaccggtcga aggcctcgcc
     7801 gtccgtcccc ttcaccagct ccatgagctg gtcgcgcgac agcacccggc cggggtgccg
     7861 ggccagcgcc agcagcaaat cgagctggta gctggtgagc gaccgctcct cgccgtcgac
     7921 ccgcgcccgg cgcgagcccg ggtcgacctc gagccggccg aagcgcaggc tcgcttccgc
     7981 ctcgccccgg ccgcgccgca ggatcgccct gagccgtgcc aggagctcgc gcgggttgaa
     8041 gggcttcggc agatagtcgt cggcaccgag ctcgagcccg acgatgcggt ccgtctcgtc
     8101 gccccgcgcc gtgagcatga gaaccggcac ggtggagacc cgccgcagcc gccggcagac
     8161 ctcgaagccg tcgagatccg gcagcatcac gtcgagcacc accacgtccg gcgcccgccg
     8221 cgccagctcg gcgaggccgg ccgagcccgt cgcggcggtg cggaccgcat agcccgcccg
     8281 ggtcaggtag tcgcggagca tggcggcgag cctgaggtcg tcgtcgacga tcagcacctc
     8341 gcccgccatg ccggacatcg gcgccgtgcc gccggtcagt ccaggccgcg ccggccgcgg
     8401 cgctcctcga gccgtgccgc cagcgccgcc cgctgcgccg ggtcgagcac ctcggccgcc
     8461 tccagcagag ccgccacggc gcgcttcgag gcgtcgtcca gcatcgccat ccgcgagctg
     8521 cgcagctcct ccaccgctgc cttgtcgatc accggctcca ccaggagcgc cgccagctcg
     8581 ccccgcgccc cgcgcagcgc ctccatcatc ggcgccagct cgtcatgcgt cgcctcggca
     8641 atcgccgtga tccgggcctg ctgctccggc ctcgcgtcca ccgcatcgag cgcgcgcgtc
     8701 agccgccact cggcgaagtc gcgcatgccg ccatgatgcc agccgccatg ccagccgctg
     8761 cccgtggcgg ccacgacgcc ggctccgaca cccaccgcga ccaccagcac ggcaccgccg
     8821 atcaggatgc ggtggcccca gcgcgtcggc gcccggtgct gcccgccccc ggcttgcccg
     8881 gtcccgttcg tgtccttgcc gtcagtcatc ggtcattctc cgcaaacgca cggccccacc
     8941 gaccgcgttg caggcaagct ggccccccac catgtcggcc atgccggccg cgtgtaaaga
     9001 attgtgaagc gcctgccggc atgcgcgttc gcgcccccct ttcgcaccac ccggctgtca
     9061 ctcgggcctc tgcaagtggc ccggctgccg ttctcaccga cttttccaga aatccacccg
     9121 ccagcccttg acaggccagc ccggaacggt ctctcacacc tcaacccgac cggaccgcgc
     9181 gcacccgatg gatatctatc tccccgtcgc cgagatgtcg gtgaacctct tcctcatcct
     9241 cggcctcggc gggatggtgg gattcctctc gggcgtcttc ggcgtcggcg gcggcttcat
     9301 catgaccccg ctcctgatct tcatcggcat cccgccggcc gtcgccgtcg gctcgggcgc
     9361 ctcgcagatc ctcgcatcct cgacctcggg ggcgatcgcc cacacccgcc ggcgcaccgt
     9421 cgacttccgc atgggctggg tgctcatcgc cggcggtgcc gtcggctccg gcctcggcat
     9481 cctgctgttc cgctggctgc gcgccacggg ccagatcgac gtcgtcatcg cgctcgccta
     9541 cgtcctcttc ctcggcgtca tcggtgcctt gatgttcgcc gagagcgccc gcaccctctt
     9601 ccgcacccgc aagtccagcc ccgccacccg tcgccgcaag ctgcaccagc accactggct
     9661 gcacggcctg cccatgaaga tgcgcttcaa tcagtcgcgg ctctatatca gcgtgctcac
     9721 cccgctcgcc gtcggcgccg gcgtcggcgt cctcgtcgcc atcatgggtg tcggcggcgg
     9781 cttcatcatg gtccccgcca tgatctacct gatcggcatg ccgaccagcg tcgtcatcgg
     9841 cacctcgctc ttccagatcg ccttcgtcac cgcgatcacc agcttcctcc acgcctacac
     9901 caaccaggcc gtcgacatcg tcctggccct cctgctcatc gccggcggcg tcatcggcgt
     9961 ccagttcggc tcgcgcgccg gcgcggtcct caagggcgag cagctccgca tcctgctcgc
    10021 gatcctcgtc ctcgccgtcg ccctgaagat ggcctgggac ctcgtcgcca cccccgccga
    10081 cgtcttctcc atcgcccgct gagcgacgca tggcgaaagg cacgccagcc gtccaggcgc
    10141 tgacgcgcgc aaagatcgcg ttcaccctgc acgagtacga tttcgtcgcc acaccgggcg
    10201 ccatcgggct ccaggcggcc ctggccatgg gcgtcgcccc cgagcgggtc caaaagacgc
    10261 tgatggcggt ggtcgacgag caggagctcg tcgtggccgt catcgccgtc gaccgcgagc
    10321 tcgacctcaa ggccctggcg aaggccgcgg gcggcaagcg ggccgccatg gcggaggtgg
    10381 cccgggccga gcgggcgacc ggctacgtca agggcggcat cagccccttc ggccagaaga
    10441 agcgccaccg cacctttctc gacgccacgg cgctggcgct gcccaccatc atcgtcaatg
    10501 gcggccggcg cggcctccag atcgagctgg cgcccgcgga tcttctggca gccaccgccg
    10561 gcacggccac ggcgctcgcc cgcgtcatct agcgacctcg tcttttgccg ccggtcgtga
    10621 tctgattccc ccgttcaggc ttcgatgatg cgcgcgggga tgcaccaatg agtgacacga
    10681 agctcgctct cgccgccgat tttccaccgg ccgacccggc ggcatggcga gagctggtcg
    10741 agaagaccct gaagggcgcg gacttcgccg cgaccctggt ctcccgcacc gacgacggga
    10801 tcgaggtcca gccgctctac accactagcg cggcccggcc cggcctgccc ggcagcccgc
    10861 cccacctgcg cggtgcgacc gccgccggcc acgtcgccgg cgggtgggac atccgccagc
    10921 tccacgatca cccggacccg gctttggcca accgtgccat cctcgaggac ctcgagcagg
    10981 gcgtcacctc gatcgtgctg cggctggatg cggccttcgc gcagggcgag gacgagccgg
    11041 acggcatcct cgcccggaac gccgacgggc tcgaccgggt gctcgacggg gtcatgctcg
    11101 acctcgcacc ggtccagctc atggcgcccg gccacaccgt cccggccgcc cgagcgctgc
    11161 tcgagctcct gcgccggcgc gaccatgcgc cagataccgt caccgccgat ctcgggctcg
    11221 atcccatggg tgccgtcgcc ctcggccgtg ccgatccgac ggacgccctg ccaccggcct
    11281 tcgacctggt ccggcaggtg gcgggcgaat ggcgcaagct cgaccttctt cgggtcgacg
    11341 gcgagctcta ccatgcggcg ggcgccagcg aggcccagga gctggcggcc gccatcgcca
    11401 ccggcatcca gtacctgcgc gaaggcgagg cggtcggctt ggagccggca agcctcgccc
    11461 ggcggctcgg cttcaccctc gccgccgatg ccgatctctt cctcaccatc gccaagtgcc
    11521 gggccgcacg gcggctctgg ccccgggtga tgaccgcctg cggcgccgat gacgtgccga
    11581 tgcggctcga tctgcgcacc gccagccgga tgctgacccg ccgcgatccc tgggtgaaca
    11641 tgctgcggac cacggtcgcc tgcctcgccg gcgtggccgg cggggccgag gccatcaccg
    11701 tcgccccctt cgatgccgcc ctcggcgagc ccggcaggct cggccggcgc atcgcccgca
    11761 acgtccagct cgtcctgatg gaggagtgca acctccaccg cgtcatcgac ccggccggcg
    11821 gcagccccta tgtcgagacg ctgagccttg cgctcgccga gcgggcctgg ccgctcgtcc
    11881 aggcgatcga gggcgagggc ggcatgctgg cgagcctccg ccagggcatc gtccaggaca
    11941 gcatcgccgc gacctgcctc gcccgcctcg acgacctcgc ccaccgccgc cgggcgatca
    12001 ccggtgtgag cagcttcccc gatctcgacg agcagacgct gccgcccgcc gagccggatc
    12061 tcggccccct cctcgagcag gcggccgcct ccctgccgcc acgcacgaag ccggccgcaa
    12121 ccgtggccgc cctcaccccc atgcgcctcg ccgagggctt cgagcgactg cgcgacctcg
    12181 gcgacctcgc cctgatcgag cacgggcagc ggccccgcgt cttcgtcgcc gccatgggcc
    12241 cgctcgcccg gcacggcgcg gaggcgagct tcgttcgcaa cgccctggcc gccggcggca
    12301 tcgaggcgat tgtcagcccg ccgctcgagg acagcgacgc ggcagcgtct gccttcgccg
    12361 aaagcggcac gaccatcgcc tgcctctgcg gcatcgaccg gacgaaaccg gccaccgccg
    12421 ggcccatcgc ccgggcgctt ctcgatggcg gggcgatcgc ggtctatgcc gccgggcggc
    12481 ccgatccggc gctgagggcc gagggcatcg acggcttcgt gcacacgggt gccaatatcc
    12541 tgggcctcct cgaggacctc cactccctgg cgctgggaga agctgcatga cccgggttcc
    12601 cgacttcacc cggctgccgc tcgacgacgg accggtgacc gcgcgcggcg actgggcgtc
    12661 cgccttcctt gccgagaccg ggcagacacc cgaagccctc gcctgggcca cgccggagga
    12721 gatcgacgtc cgcccgctct acggcccgga cgacctcgcc caggtcgacc acctcgacgg
    12781 cctgcccggc ctgcccccct tcgtgcgcgg cccctacccc accatgtatg tcggccagcc
    12841 ctggaccatc cgccagtatg cggggttctc cacggccgag gaatccaacg ccttctaccg
    12901 gcggaacctc gccgccggcc agaagggcct ctccatcgcc ttcgacctcg ccacccatcg
    12961 tggctacgac agcgaccacc ccagggtggc gggcgatgtc ggcatggccg gcgtcgcgat
    13021 cgattcgatc ctcgacatgc gccagctctt ccacggcatc ccgctcgacc ggatgagcgt
    13081 gtcgatgacc atgaacggcg ccgtcctgcc cgtcctcgcc ctctacatcc tcgccggcga
    13141 ggagcagggc gtggcgccgg agcagctcgc cgggaccatc cagaacgaca tcctcaagga
    13201 gttcatggtc cgcaacacct acatcttccc gcccaagccc tcgatgcgga tcatctccga
    13261 catcttccgc ttcaccgccg agcgcatgcc gaagttcaac tcgatcagca tctccggcta
    13321 tcacatgcag gaggccgggg cgacggccga cctcgagctc gcctacaccc tggcggacgg
    13381 gatcgagtac ctgcgcaccg gcgaggccgc cggcctcgac atcgaccgct tcgcgccccg
    13441 gctcagcttc ttctgggcca tcggcatggg ctacttcatg gaggtggcga agctgcgggc
    13501 cgcccgcctc ctctgggcga agctggtgcg ccccttcgag cccagggacc cgcgctcgct
    13561 ctcgctccgc acccactgcc agacctcggg ctggtccctg actgcgcagg acgtcatgaa
    13621 caatgtcgtc cgcacctgtg tcgaggcgct ggccgccacg catggcggca cccagtcgct
    13681 gcacaccaac gccttcgacg aggcgctggc gctgccgtcc gagttctcgg cccggatcgc
    13741 ccgcaacacc cagctcttca tccaggacga gaccggcatc cgccggaccg tcgacccctg
    13801 gggcggcagc ttcttcgtcg agcgcctgac ccacgacctc gccgcccggg cctggggcca
    13861 tatcgaggag gtcgagcagg cgggcggcat ggcgaaggcc atcgagcagg gcatccccaa
    13921 gctcaggatc gaggaggcgg cggcccgcac ccaggcccga atcgacagcg gccggcagac
    13981 cgtggtcggc gtcaacaagt accggccggg cgagcccgag catgtcgacg tgctcaaggt
    14041 cgacaaccgg gcggtgcgcg ccagccagat cgcgaagctg gagcagctcc gcaagggccg
    14101 cgacgagccg gcgctgcgcc gcgccctcga tgcgctcacc cgctcggccg gcaccggcga
    14161 gggcaacctc ctggcgctgg ccgtcgacgc cgcccgggcc agcgccacgg tcggcgagat
    14221 cacgagtgcc ctcgagcagg tctggggccg ccacgtcgcc gagatccgct cgatcaccgg
    14281 cgtctacggc agggaggtgg gggcgatgga cagtgtccgg gcatgccgcg agcgggtcag
    14341 ggccttcgcc gaggccgaag gtcgccggcc gcgcatcctg atcgccaaga tgggccagga
    14401 cggccacgac cgcggccaga aggtgatcgc ctcggccttc gccgatc
//
LOCUS       DAOTFI010000123        14434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5031_length_14434_cov_6.893664, whole genome shotgun sequence.
ACCESSION   DAOTFI010000123 DAOTFI010000000
VERSION     DAOTFI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14434
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5031_length_14434_cov_6.893664"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="P5558_13480"
     CDS             <1..491
                     /locus_tag="P5558_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25369.1"
                     /translation="SVHLSHLDAATRLPQIEALLAIHRRAPREGGAWCGGHPNPAAGW
                     TEGEMPPMPRDAVFMGDFNFVPGSDEYTAMVGPLTARYGRLLNTDGLVDAWTVAGHDE
                     GAGVTCDGKRLDYLFLSADLRCRVRSVRIDETAEGSDHLPVWMDFDAGRGASREILGQ
                     CE"
     gene            482..1462
                     /locus_tag="P5558_13485"
     CDS             482..1462
                     /locus_tag="P5558_13485"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRY25370.1"
                     /translation="MRIAVFGAGAVGGFLAAHLARAGRDVSVIARGAQLDAIRADGLT
                     LELPHETFDVALEASDDPGTVGPVDLVLVTVKTTANPAIAAAMAPLLHDGTVVVFAQN
                     GVFWWYGLGFDPGVPVDAARLDPEGRLAATLGPDRVLGLVIYSPNEVIAPGRIRCTVS
                     DSRFHLGGPDRAAGQVARAAAVLGDAGFRLEVPADIRAAMWQKLVLNLCTSPLSTLTR
                     ATEAGLESLPGMIEVGRGMVRDGLAVAAAHGFRDLGIDPEKLVAPGLRTPHRPSMLQD
                     LERGRPLEIDSLLAIVQDFARAAGVATPTIDTVLPLLILLARGRGLYPAG"
     gene            1711..2097
                     /locus_tag="P5558_13490"
     CDS             1711..2097
                     /locus_tag="P5558_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25371.1"
                     /translation="MRGSTAMMAVLLSLGSAALAEDGRPATRPALVWAGVEHSRVDLA
                     HVKGTEPLVGEHGFGALRDDWQPGQEVAWQHGAIGLGLREENGRIKGGPYLQAGDWRL
                     DLRPVGSDNALDIDGVTLRLTTKFGD"
     gene            complement(2108..3139)
                     /locus_tag="P5558_13495"
     CDS             complement(2108..3139)
                     /locus_tag="P5558_13495"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY25372.1"
                     /translation="MKGGSRSRLGLALLLGVWAVAASLASCSPDGADLDRQALDVPAT
                     CADLAERGSLRVLTLNLHFSAIRERPRRLETISDFIAAEGVDVALLQEAVGGVLSGSR
                     NSARDLRDALSDRHGLAFDLRTAVEDGVPGVLTVANAILSRCAVLEARTAALPEIAEL
                     EVLGHRFRLGRNVVMARLLVPKIGEISVYNTHLCARCTADQRRRQLVEALAFIGANEE
                     GGGRPVIFGGDLNLDLHGADDAERALYGLMAEAGLRDAASAVTGAVGQLCAQPARPDA
                     YCTVGVAEPTRPHSKRIDYLFARDFGGILAYRTVFNPRLDPTQTAVSNHAGVMVELDL
                     TTGRALARP"
     gene            complement(3167..3454)
                     /locus_tag="P5558_13500"
     CDS             complement(3167..3454)
                     /locus_tag="P5558_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRY25373.1"
                     /translation="MSGSTALRLAATDNVATALRDLEAGEVVEIEDQRIELLERIPLC
                     HKLALADLAPGTPVTKYGQPIGRTTTAVARGAHVHVHNMKSARAQAPRPAR"
     gene            complement(3451..4608)
                     /locus_tag="P5558_13505"
     CDS             complement(3451..4608)
                     /locus_tag="P5558_13505"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRY25374.1"
                     /translation="MRPFLGFRRASGRPGVRNHTLVLSVTGLTTPAARRIARSLNGVV
                     VLGTQHDSGLLGYDRKVHRGALAAFACHPNVGGVVVIGANAPVVEQVAAAAEAAGRLV
                     EAITLDACGHDVLTLSDKALRAAAGFVHGLSHQRREELPPAGLLLGLECGRSDPSSGL
                     VANPLVGAVVDRLVDAGGAAIFGETTEWIGAEEPLVRKARDATVAERLKAAAARRESM
                     AQAAGIDLTGNNPSQTNIDAGLSSIEEKSLGAIAKAGSRPIQGVIGYGEPPPGPGVWA
                     MDAPAYAPESLTGFSTAGANMALFTTGVGNSYVSALMPTLKLTGNPATARRVGQQLEF
                     EASAVFTGEESLDAAADRMVDRLLEIASGALTWGEILGEGDEAISRFGAVL"
     gene            4649..5935
                     /locus_tag="P5558_13510"
     CDS             4649..5935
                     /locus_tag="P5558_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25375.1"
                     /translation="MEHPVFPAVRIPHLAEAELPLVHRVKLEQPTAPAIDDIEAAVHE
                     ALAGSAEIAALKPGSAVAIGVGSRGVADLPRLVGATVTYLKNQNLAPFIVPAMGSHGG
                     ATAEGQALVLSRLGIDEARVGAPVRASMETVELGRTKGGLPCRFDANAAAADAIVVIA
                     RVKPHTSFDRPIESGLCKILAVGLGKEAGARNVHRLGPRGFTETIPELASIALARAPI
                     ACGLAVVENATHRLVTIEAVAPGGFVATDERLLKRAKSLMARLPFRLVDALLVEELGK
                     EISGSGMDYAVTGRTDIRGIANPDHILVNKLAVLRLTEATHGNAIGVGIADYMPRESA
                     NQVDLVAMYQNAITAAIGEKARLPIVLPTERETVQALVMTSWADDRAGCRYCQIRNTL
                     TLDEVLVSAPLLAELGQDAKAEKLRFDEAGRLLTLV"
     gene            complement(5943..7445)
                     /locus_tag="P5558_13515"
     CDS             complement(5943..7445)
                     /locus_tag="P5558_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY25376.1"
                     /translation="MEAVLSGLMGGLGLVFAPMAFFFVVLGTVLGIVVGAIPGLTGAM
                     LIALTLPLTYYMVPTDAVVLLIGMYVGSITGGLITATLLRMPGTPSNVMTTFDGYPMA
                     RAGRPGRALGLGIAASFAGGIISWIFLLLLAKPLSIWATRFGPFEYFTLILMAMVLIA
                     SVSQGNLVKGLVAGLLGMLISMPGVDPSAGQPRLTFDWYMLNGGLKLLPVLIGVFAVS
                     QIISDVLDLGRKIERVESQRAGLLLPLRDWIRHGANMLRSGLIGTLVGILPGVGASIG
                     SVIAYTTTKNLSKTPDEFGKGSEAGIVASETANNATVGGALIPLIAMGIPGSVIDAIL
                     IGALMIHSITPGPALFITNGDVVYAMIAACLVANFVMLAVMVASTGWVAKLAYVPRTY
                     LLPVIIVFCVIGSFGLDNVMFDVWVMLIFGVIGFALERARYPLGPFVIGFVLAPLMEA
                     KLRTGLMMTGGTITPMFTRPIALAFLVVAIVLLVWPLVGEWRRRRAAAAA"
     gene            complement(7466..7930)
                     /locus_tag="P5558_13520"
     CDS             complement(7466..7930)
                     /locus_tag="P5558_13520"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY25377.1"
                     /translation="MQDRVDPRVEIGVALMVIVIAAIALFETRDIPPGSFEPLGSAPV
                     PQATAGLIILMALGLIVRACLDRRPVARPPREGLADAGAVLLLTLLYVTAMHYRLTTF
                     AVMTTIYLVLTIGTLIRFRPRSMPLVLALAAVVGFGSQYVFTRVFVVDLPGL"
     gene            complement(7996..9003)
                     /locus_tag="P5558_13525"
     CDS             complement(7996..9003)
                     /locus_tag="P5558_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY25378.1"
                     /translation="MTTMTRRTALRGAALALMATLLGASFGSGPAQAEWPEKPIKVIV
                     PFQPGGSSDQVTRAYQMAIQENDILPQPITVVNVGGHFSIGARQVMEAEPDGYTFLTL
                     HIALMGGEAGGMFDFGYRDFTPVAATGTFCLVPVVRKDSGIESVGDLLAKAKAEPDTL
                     IFGANLGAINHMAGIMLQNLEEGAEFRFVQIGGGTANFTALTGNQTNVSVLSAAEILN
                     FTMLPDGSENPESEIRPLAYTGAERAAKLPDLPTMEELGYDMQFCVDSWWFAPKGTPE
                     EAVAGFADALEKATDTPQVQAFFDEKAFGAVFLRGDELQASLDETWARIEPVAKQAAQ
                     K"
     gene            complement(9097..10050)
                     /locus_tag="P5558_13530"
     CDS             complement(9097..10050)
                     /locus_tag="P5558_13530"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRY25379.1"
                     /translation="MELRNIEYFLTVAAEGSMRAAAKKLGLTQPALTKAMHRLEDEAG
                     VTLFDRRSRGVSLTVYGESFRRHARTIRAGMAEAESEIQALRNGTSGLVRIGAGPSWQ
                     SRVIPEAIRRFRAGRPGVQLRVVGGTDDVLKAELRAGHLDLVVAAVPEGEEAPDLEGR
                     ALLVDEYRVIADKSHPLQTATDSTVANLLDYPWILPNPQTYLVRRLEVLMRAHGLTPP
                     RPMIETDIIDIKLALMRDSAYLSFHAVDHLRSTGVSEIRPLPLEAASWRRSAGVITRR
                     GVEANPAAAHLADLIEELCRRDQQPAPKRGRVARAGRLVAG"
     gene            10203..11195
                     /locus_tag="P5558_13535"
     CDS             10203..11195
                     /locus_tag="P5558_13535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY25380.1"
                     /translation="MADKMSRARRAVLGYGIAILAAAAFGGLGGQAYADYPEKPIRVI
                     VPTQAGGGMDSVARIFQRYFDEHGDVLGQKMVVVNMDGAGGTVGTRAIRDAEPDGYTI
                     GIWHEGLITSKVMGVVDYDHAAFELLGVTGYGDLGFGVGKDSPYQTFEALLDAARAAP
                     NTIKCATNIGLPVHFVPLMVQDEAGVEFRYVQVGGGAKRFPSVVAGHTDFAIFGAIEL
                     VKWTDADLVPIVMFASERNPLLPDVPTARELGVDVVARNNRIWLGPKGMPDEVVAHLN
                     NAFRTAMADPAIQEQMVDQGLVPVFVEPDALLEELDWWAEKAGPLVAKARELQQ"
     gene            11211..11744
                     /locus_tag="P5558_13540"
     CDS             11211..11744
                     /locus_tag="P5558_13540"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY25381.1"
                     /translation="MDLRRRLWVDAAGRRDLLLALAVILLALVVYWQASLLPPPFFDP
                     VGSAAVPKFVALLLALLAVGVIVERLRAKPGAVEAGFAPEVIDDDPAEPAPWTALASI
                     VLPVLYVAVMQAGWLAFAQASTLFVLALGALFGRFRPRLLLLLVPVAFLAGYGLDYIF
                     TEILYVDLPQRSLLGGS"
     gene            11748..13253
                     /locus_tag="P5558_13545"
     CDS             11748..13253
                     /locus_tag="P5558_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY25382.1"
                     /translation="MLGDLGSALLYIAQPWPFFFVLLGTSLGVFVGAVPGLTGGMLIA
                     LVMPLTFYMDSTLALVMMVAMYVGSVSGGLISATLLRMPGTPSSVMTTFDGYPMAQQG
                     QPERALALGIGSSLIGGLIAGVVLVLLSPPLSKWAMTFSPWEYFTMVLMAIVLIASIS
                     QGSMIKGLIAGFLGMVMAMPGLNPSDGQLRLTFGIDAFADGFKLLPVLLGAFVMSQVI
                     KDALEINKRPVMLKLGAGRVLVPLREWRSHWVNILRSSVIGTWIGILPGVGASISAMV
                     AYVTAKNLSRTPEKFGTGHAEGIVASEAANNANVGGALIPLITMGIPGSPIDAILLSA
                     LILHNIQPGPLLFITNGEFVWALMAAYLVANVVMFLLMTLSIRWIARVVTVEKAFILP
                     IIFVCCVIGAYALSNRMYDVWVVIAFGVIGFLLDRAKVPLGPFVIGFVLAGIFEAELR
                     SSLQMSDNGILGILERPIAMTFVLVSVVMLVWPFVQSWRQRRSATASETTR"
     gene            13250..>14434
                     /locus_tag="P5558_13550"
     CDS             13250..>14434
                     /locus_tag="P5558_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY25383.1"
                     /translation="MTPSNVLFIMSDEHSRRHLGAYDDTFVRTPSMDALAAQGTLFEN
                     AYCNCPICVPSRASFATGRHVHEIGNWDNASPYQGEPPSFGHTLAATGHRCDSIGKLH
                     YRSAEDPNGFDNEILPLHVLNGKGDVQGILRSPPPVREATKQLAADAGVGDSTYLDYD
                     RKIRDAACEWLEETAKAPPDKPWMLFVSFVCPHFPLVAPKEFFDLYPLDQVPMPRLTG
                     PDELPDHPVLRMFRKVQNYQDHFRDEAHVRTAIAAYLGMVSFLDDNIGRLLRTLDATG
                     LGRDTVVVYTSDHGDNLGTRTFWGKSNMYDESAGVPLIMAGPGVPVGRRVKTPVSLVD
                     AHPTILEVMGEASDPALPGRSLLAIAEGAEPDRTVFAEYHAVASITGIFMVRFGRYKH
                     IHY"
BASE COUNT         2118 a         4878 c         5215 g         2223 t
ORIGIN      
        1 attccgtgca tctgagccac ctcgatgcgg cgacccgcct gccgcagatc gaggcattgc
       61 tcgcgatcca tcgccgggca ccccgcgaag gtggtgcctg gtgcggcggg cacccgaatc
      121 cggccgccgg ctggaccgag ggcgagatgc cgcccatgcc gcgcgacgcc gtcttcatgg
      181 gcgacttcaa cttcgtgccc ggcagcgacg agtacaccgc catggtcggg ccgctgacgg
      241 cgcgctacgg ccggctcctc aacaccgacg gtctggtcga tgcctggacg gtcgccggcc
      301 acgacgaagg cgccggcgtc acctgcgacg gcaagcggct ggactatctc ttcctctcgg
      361 ccgatctacg gtgccgggtg cggtcggtcc ggatcgacga gacggccgag ggctcggacc
      421 acctgccggt ctggatggac ttcgacgcgg gccgaggggc aagccgggag attctcgggc
      481 agtgcgaata gcggtctttg gcgccggcgc ggtcggcggc tttctggcgg cgcatctggc
      541 gcgggccggg cgcgacgttt cggtgatcgc gcggggtgcg cagctcgatg cgatccgcgc
      601 ggacgggctc acgctcgagc tgccgcacga gacgttcgac gtggcgctcg aggcgagcga
      661 cgatccgggc acggtcggcc cggtcgatct cgtgctggtg acggtcaaga ccacggccaa
      721 cccggcaatc gcggccgcca tggcaccgct cctgcacgat ggaacggtgg tggtgttcgc
      781 gcagaacggc gttttctggt ggtacggcct gggcttcgat ccgggtgtgc cggtcgatgc
      841 ggcacggctc gatccggaag ggcggctcgc cgccacgctc ggaccggatc gtgttctggg
      901 gctcgtcatc tactcgccca acgaggtgat cgcacccggg cggatccgct gcacggtgtc
      961 ggacagccgg ttccacctgg gcgggcccga cagggcggcc gggcaggtcg cgcgggcggc
     1021 ggcagttctg ggcgatgccg gattccggct cgaggtgccg gccgacattc gtgccgcgat
     1081 gtggcagaag ctggttctca atctctgcac ctcgccgctt tcgaccctga cgcgcgccac
     1141 cgaggccggg ctggaatcgc tgccgggcat gatcgaggtc gggcggggga tggtgcggga
     1201 cgggctcgcc gtggcggcgg cgcacggttt tcgcgatctc ggcatcgatc cggagaagct
     1261 ggtggcgccg ggcctgcgca cgccgcaccg gccctcgatg ctgcaggacc tcgagcgcgg
     1321 ccggccgctc gagatcgatt ccctcctcgc cattgtccag gacttcgccc gtgccgccgg
     1381 cgtggcgacg ccgacgatcg acacggtcct gccattgctg atccttctcg cgcgcggccg
     1441 ggggctctac ccggcgggct gagcggcctc gaccccgcac ggcaaggtgt ggatgcgccg
     1501 ggtgtgaacg cggtcacccg gcgcccagct gacggcagct cgaagggtcg aagtcggttc
     1561 gtgtcccgat ttttcccgtg tcccgatttt tcccaaccgc ttgccgcgcc aaggggatcg
     1621 gcctaagctt cgaatcattc caaaaaggct catcgatgga gatgctggcc gggccgtccg
     1681 gtcggcgtgg cgggtgcagg ggagacgatc atgcgtggct cgaccgcaat gatggccgtg
     1741 ctgttgtcgc tgggttcggc cgccctggcc gaggacggcc ggccggcgac cagaccagcg
     1801 ctggtctggg ccggcgtcga gcattcgagg gtcgatctgg cgcacgtcaa gggcaccgag
     1861 cccctggtcg gcgagcacgg cttcggcgcg ctgcgggacg attggcagcc cggccaggag
     1921 gtcgcctggc aacacggcgc cattggcctc gggcttcgcg aggagaacgg gcggatcaag
     1981 ggtggcccct acctccaggc gggggactgg cgcctggatc tgcgcccggt cggcagcgac
     2041 aacgcgctcg acatcgacgg tgtcacgctg cgtctcacga ccaagttcgg cgactgaccg
     2101 cgtctcgtca cgggcgggcg agagcgcggc cggtggtcaa atccagctcg accatcacgc
     2161 cggcgtggtt ggagacggcg gtctgggtgg gatcgagacg cgggttgaac acggtccgat
     2221 aggcgaggat gccgccgaaa tcgcgcgcga aaaggtagtc gatgcgcttc gagtggggac
     2281 gggtgggctc ggcgacgccg accgtgcagt aggcgtccgg ccgggcgggc tgggcgcaca
     2341 actggccgac cgcgccggtg accgcgctcg ccgcgtcgcg caggccggct tcggccatca
     2401 gcccgtagag cgcccgctcg gcatcatccg cgccgtgcag gtcgagattg agatcgccac
     2461 cgaagatcac cggccgcccc ccgccctcct cgttggcgcc gatgaaggcg agcgcctcga
     2521 cgagctgccg gcggcgctgg tcggcggtgc agcgggcgca gaggtgggtg ttgtagacgc
     2581 tgatctcgcc gatctttggg acgaggaggc gggccatcac gacgttgcgg ccgagccgga
     2641 agcgatggcc aaggacctcg agctcggcga tctccggcag cgctgccgtt cgcgcctcga
     2701 gcacggcgca gcggctcagg atggcgttgg cgacggtgag cacgcccggc acgccgtcct
     2761 cgaccgccgt tcgaaggtcg aaggcgaggc cgtggcggtc gctcagcgca tcccgcaggt
     2821 cgcgggccga gttgcggctg ccggacagga cgccgcccac ggcctcctgc aagagggcga
     2881 catcgacgcc ctccgcggcg atgaagtcgc tgatggtctc gagacgacgg ggccgctcgc
     2941 ggatcgccga aaagtgcaga ttgagggtca ggaccctgag gctgccacgc tcggcgaggt
     3001 ccgcgcatgt cgccgggacg tcgagggctt gccggtcgag gtcggcgccg tcgggcgagc
     3061 agctcgccag ggaggccgcg accgcccaga cgccgagcag aagcgccagc ccgagccgcg
     3121 agcgggaccc tcccttcacg accccgccgg cgcgggagcg ggggccctat cgggccggcc
     3181 gaggggcctg ggcacgggcg ctcttcatgt tgtggacgtg gacatgggcg cccctggcga
     3241 ccgccgtcgt cgtgcggccg atgggctggc cgtacttggt gacgggtgtg cccggggcga
     3301 ggtcggcaag cgccagcttg tggcagaggg ggatgcgctc caggagctcg atcctctggt
     3361 cctcgatctc gaccacctcg ccggcctcga ggtcgcggag cgccgtcgcc acgttgtcgg
     3421 tggcggcgag gcgaagcgcg gtgctgccgc tcacaggacg gctccgaagc ggctgatcgc
     3481 ctcgtcgccc tcgcccagga tctcgcccca ggtgagggca ccggaggcga tctcgagcag
     3541 gcggtcgacc atgcggtcgg cggcggcatc gaggctttcc tcgccggtga agacggcgct
     3601 ggcctcgaac tcgagctgct ggccgacgcg ccgggcggtg gccgggttgc cggtgagctt
     3661 cagggtgggc atgagggcgc tcacatagct gttgccgacg ccggtggtga agagcgccat
     3721 attggccccg gccgtgctga agccggtaag cgattcgggc gcgtaagccg gggcgtccat
     3781 ggcccacacg cccgggccgg gcggcggctc gccatagccg atgaccccct ggatggggcg
     3841 gctgccggcc ttggcgatgg cacccaatga cttctcctcg atcgaggaga gcccggcatc
     3901 gatgttggtc tggctcgggt tgttgccggt gaggtcgatg ccggcggcct gggccatgga
     3961 ttcgcgccgg gccgccgccg ccttcagccg ctcggcgacc gtggcgtcgc gggccttgcg
     4021 cacgagcggc tcctccgcac cgatccattc ggtcgtctcg ccgaagatcg cggccccgcc
     4081 ggcatccacc aagcggtcga ccaccgcacc gacgagcggg ttggcgacga ggccggagct
     4141 cgggtcggag cggccgcatt cgaggccgag caggagcccg gccggcggca gctcctcgcg
     4201 gcgctggtgg gagagcccgt ggacgaagcc ggcggcggcc cgaagggcct tgtcggaaag
     4261 ggtgagcacg tcgtggccgc aagcgtcgag ggtgatcgcc tcgaccagcc ggccggcggc
     4321 ctcggcggcg gcggcgacct gctccaccac cggcgcgttg gcgccgatga ccaccacacc
     4381 gccgacattg ggatggcagg cgaaggcggc aagggcaccg cggtgcacct tgcggtcata
     4441 gccgagcagg cccgaatcgt gctgcgtgcc gagcacgacg acaccgttca aggatctggc
     4501 gatgcgccgg gcggccgggg tggtgagccc ggtgaccgac aggaccagcg tgtggttgcg
     4561 cacgccgggc cgccccgagg ctcgacgaaa gccgaggaat gggcgcatga tgggtccttc
     4621 ccttgcaaca gaccgtcgag gccccggcat ggagcacccc gtctttcccg cggtccgcat
     4681 cccgcatctg gccgaggccg agctgccgct cgtccatcgg gtgaagctcg agcagccgac
     4741 cgccccggcg atcgacgaca tcgaggccgc cgtgcacgag gccctggccg gcagcgccga
     4801 gattgccgcc ctgaagcccg gcagcgccgt tgcgatcggg gtcggcagcc gcggtgtcgc
     4861 cgacctgccg cgtctggtcg gtgccaccgt cacctatctc aaaaaccaga acctcgcacc
     4921 cttcatcgtg ccggccatgg gcagccatgg cggggccacg gcggagggcc aggcgctcgt
     4981 gctctcgcgg ctcggcatcg acgaggcgcg cgtcggggcg ccggtccggg cgagcatgga
     5041 gacggtcgag ctcggccgga cgaagggcgg gctgccctgc cgcttcgatg ccaacgcggc
     5101 ggcggcggac gccatcgtcg tgatcgcccg ggtcaagccg cacaccagct tcgaccggcc
     5161 gatcgagagc gggctctgca agatcctggc ggtcggcctc ggcaaggagg cgggcgcgcg
     5221 caatgtccac cggctggggc cgcgcggctt caccgagacg atcccggagc tggcctccat
     5281 tgcgcttgcc agggcgccca tcgcctgcgg cctcgccgtg gtcgagaacg cgacgcacag
     5341 gctcgtgacc atcgaggccg tggccccggg cggcttcgtc gccaccgacg agcggctgct
     5401 caaacgggcg aagtcgctca tggcgcgcct gccgttccgg ctcgtcgacg cgctcctcgt
     5461 cgaggagctg ggcaaggaga tctcgggctc gggcatggac tacgcggtga ccggccggac
     5521 cgacatccgg ggcatcgcca accccgacca catcctggtc aacaagctgg ccgtcctgcg
     5581 gctgaccgag gcgacccatg gcaacgccat cggcgtcggc atcgccgact acatgccgcg
     5641 cgaatcggcc aaccaggtcg atctggtcgc catgtaccag aacgcgatca ccgcggcgat
     5701 cggcgagaag gcgcggctgc ccatcgtcct gccgaccgag cgggagaccg tgcaggccct
     5761 ggtcatgacg agctgggcgg acgaccgggc gggctgccgc tactgccaga tcaggaacac
     5821 gctgaccctg gacgaggtgc tggtctcggc cccgctgctc gccgagctgg ggcaggacgc
     5881 gaaggccgag aagctgcggt tcgacgaggc gggccggctg ctcacgctcg tctgagcggc
     5941 gctcaggcag ccgcggcggc gcggcggcgg cgccattcgc cgaccaaggg ccagaccagg
     6001 agcacgatcg cgacgacgag gaaggcgagg gcgatgggcc tcgtgaacat gggcgtgatc
     6061 gtgccgccgg tcatcatgag gccggtcctg agcttcgcct ccatcagggg ggcgaggacg
     6121 aagccgatga cgaaggggcc gagcgggtag cgggcgcgct cgagggcgaa gccgatgacg
     6181 ccgaagatca gcatcaccca gacgtcgaac atgacgttgt cgaggccgaa ggagccgatg
     6241 acgcagaaga cgatgatcac cggcaggagg taggtgcgcg gcacgtaggc gagcttcgcc
     6301 acccagccgg tgctggcgac catgaccgcc agcatgacga agttggcgac gaggcaggcg
     6361 gcgatcatgg cgtagaccac gtcgccattg gtgatgaaga gggcggggcc gggggtgatc
     6421 gagtggatca tcagggcacc gatcaggatg gcgtcgatga ccgagcccgg gatgcccatg
     6481 gcgatgagcg ggatgagggc cccgccgacc gtggcgttgt tggcggtctc cgacgcgacg
     6541 atgccggctt ccgagccctt gccgaactcg tcgggcgtct tcgagaggtt cttggtcgtg
     6601 gtgtaggcga tgaccgagcc gatcgaggcg cccacgccgg gcaggatgcc gaccagcgtg
     6661 ccgatcaggc ccgagcgcag catgttggcg ccgtgcctga tccagtcgcg gagcggcagc
     6721 agcaggccgg cgcgctgcga ttcgacccgc tcgatcttgc ggccgaggtc gagcacgtcg
     6781 ctgatgatct ggctcacggc gaagacgccg atcagcaccg gcaggagctt cagcccgccg
     6841 ttcagcatgt accagtcgaa ggtgagcctg ggctggccgg ccgaggggtc gacgcccggc
     6901 atcgagatca gcatgccgag gagcccggcg accaggccct tgaccaggtt gccctggctg
     6961 accgaggcga tcagcaccat ggccatcagg atcagcgtga aatactcgaa cgggccgaag
     7021 cgcgtggccc agatcgagag cggcttggcg agcaggagga ggaagatcca ggagatgatg
     7081 ccgccggcga aggaggcggc gatgccgagg ccgagcgcgc ggccgggccg gccggcgcgg
     7141 gccatgggat agccgtcgaa ggtggtcatg acgttggacg gcgtgccggg catgcgcagg
     7201 agcgtggcgg tgatcaggcc gccggtgatc gagcccacat acatgccgat caggaggacc
     7261 acggcgtcgg tcggcaccat gtagtaggtg agcggcaaag tgagggcgat cagcatggcg
     7321 ccggtcaggc ccgggatggc gccgacgacg atgccgagca cggtgccgag cacgacgaag
     7381 aagaaggcca tgggcgcgaa gacgaggccg aggccgccca tgaggccgga gagcacggct
     7441 tccatggctc agggttccca catgctcaaa ggccgggcag gtcgacgacg aagacgcggg
     7501 tgaagacgta ctggctgccg aagccgacca cggcggcgag cgccaggacg agcggcatgc
     7561 tgcgcgggcg aaagcggatc agggtgccga tggtcagcac caggtagatc gtggtcatca
     7621 ccgcgaaggt ggtgagccgg tagtgcatgg cggtcacgta gagcagcgtg agcagcagca
     7681 ccgcgccggc atcggcgaga ccttcccgcg gcgggcgggc gaccggccgg cggtcgaggc
     7741 aggcgcgcac gatgaggccc agagccatga ggatgatgag gccggccgtc gcctgtggca
     7801 ccggcgcgga gccgagcggc tcgaagctgc cgggcgggat gtcgcgggtc tcgaagaggg
     7861 cgatcgcggc gatgacgatg accatgagcg ccacgccgat ctcgacgcgc gggtcgacac
     7921 gatcctgcaa gcccgggcct ccctgaaaag gcggcggccg gcgacccggg agggccgccg
     7981 gccgccttgc cgatgctact tctgggcggc ctgcttggcc acgggctcga tccgcgccca
     8041 ggtctcgtcg aggctcgcct ggagctcgtc gccgcgcagg aagacggcac cgaacgcctt
     8101 ctcgtcgaag aaggcctgga cctgcggggt gtcggtcgcc ttttccagcg cgtcggcgaa
     8161 gccggccacg gcctcctccg gggtgccctt gggcgcgaac caccagctgt cgacgcagaa
     8221 ctgcatgtcg tagccgagct cctccatggt cgggaggtcg ggcagcttcg cggcgcgctc
     8281 cgccccggtg taggcgagcg gccggatctc gctctccggg ttctcgctgc cgtcgggcag
     8341 catggtgaag ttgaggatct cggccgccga cagcaccgag acgttggtct ggttgccggt
     8401 gagcgccgtg aagttggccg tgccgccgcc gatctggacg aagcggaact cggccccctc
     8461 ctcgagattc tgcagcatga tcccggccat gtggttgatg gccccgagat tggcgccgaa
     8521 gatcagcgtg tcgggctcgg ccttcgcctt ggcgaggagg tccccgaccg actcgatgcc
     8581 gctgtccttc ctgaccaccg gcacgaggca gaacgtgccg gtcgccgcca ccggcgtgaa
     8641 gtcgcggtag ccgaagtcga acatgccgcc ggcctcgccg cccatgagcg cgatgtgcag
     8701 ggtgaggaag gtgtagccgt cgggctcggc ctccatcacc tgcctggcgc cgatcgagaa
     8761 gtggccgccg acattcacca cggtgatcgg ctgcggcagg atgtcgttct cctggatcgc
     8821 catctgatag gcgcgggtca cctggtccga gctgccgccc ggctggaacg gcacgatcac
     8881 cttgatcggc ttctccggcc attcggcctg ggcaggaccg ctgccgaagc tcgcgccgag
     8941 cagcgtcgcc atcagcgcca gcgccgcgcc tcgaagggcg gtcctcctgg tcatggtggt
     9001 catcgtttct gcctcctcat ttcttggtgc gatttcgttc gccggcgact gtgccagcac
     9061 cggcccgggc cttcaacgct gctatcggat cgcctttcac ccggccacca gccgccccgc
     9121 gcgggcgacc cggccgcgtt tcggcgcggg ctgctggtcg cggcggcaga gctcctcgat
     9181 gaggtcggcg agatgggcgg ccgccgggtt ggcctcgacg ccgcggcggg tgatgacgcc
     9241 ggccgagcgc ctccagctcg cggcctccag cggcagcggg cggatttccg agacgccggt
     9301 gctgcgcagg tggtcgacgg cgtgaaagct cagataggcg ctgtcgcgca tcagcgcgag
     9361 cttgatgtcg atgatgtcgg tctcgatcat cggcctgggc ggcgtgaggc cgtgcgcccg
     9421 catcaggacc tcgaggcggc gcaccagata ggtctgcggg ttgggcagga tccagggata
     9481 gtcgagcagg ttggcgacgg tgctgtcggt ggcggtctgg aggggatggc tcttgtcggc
     9541 gatcacccgg tactcgtcga cgaggagggc gcggccctcg agatcgggcg cctcctcgcc
     9601 ctccggcacg gcggcgacca cgaggtcgag atggccggcc cgcagctccg ccttgagcac
     9661 gtcgtcggtg ccgccgacga cgcggagctg cacgcccggc cggccggcgc ggaagcggcg
     9721 gatcgcctcg gggatcaccc ggctctgcca ggacgggccg gcgccgatgc gcacgaggcc
     9781 cgaggtgccg ttgcggagcg cctggatctc gctctcggcc tcggccatgc cggcgcggat
     9841 ggtgcgcgcg tggcggcgga agctctcgcc gtagacggtg agcgagacgc cgcgcgagcg
     9901 ccggtcgaag agggtgacgc cggcctcgtc ctcgagccgg tgcatggcct tggtcagcgc
     9961 cggctgggtc aggccgagct tcttggccgc cgcgcgcatg ctgccctcgg cggcaaccgt
    10021 gaggaaatac tcgatgttgc gcagctccac cgccccgctg ccccgccttt gaaattaccg
    10081 ccagttatcg agactcttct tattggctct tgcccgatcg cgcaacatct tgtcagaatg
    10141 gccggactga atgcgtatcg gccggcgcca ttggctgcgc ccggcgaagg ggaggccaga
    10201 tcatggcaga taagatgagc cgcgcacggc gcgcggtgct cggctacggc atcgcgatcc
    10261 tcgcggcggc ggcgttcggc ggcttaggcg gccaggcgta cgccgactac ccggagaagc
    10321 ccatccgcgt gatcgtcccg acccaggccg gcggcggcat ggacagcgtg gcgcgcatct
    10381 tccagcgcta cttcgacgag cacggcgacg tgctcggcca gaagatggtc gtggtcaaca
    10441 tggacggcgc cggcggcacg gtcggcacgc gggcgatccg cgacgccgag cccgacggct
    10501 acaccatcgg catctggcac gaggggctga tcacctcgaa ggtgatgggg gtggtcgact
    10561 acgatcacgc cgcgttcgag ctcctcggcg tcaccggcta tggcgacctc ggcttcggcg
    10621 tcggcaagga ttcgccctac cagaccttcg aggcgctgct ggacgcggca cgcgccgcgc
    10681 cgaacaccat caagtgcgcc acgaatatcg gcctgccggt gcatttcgtg ccgctcatgg
    10741 tgcaggacga ggcgggcgtg gagttccgct acgtgcaggt gggcggcggc gccaagcgct
    10801 tcccatcggt ggtggcgggc cataccgact tcgccatctt cggcgccatc gagctggtca
    10861 agtggaccga tgccgacctc gtgccgatcg tgatgttcgc gagcgagcgc aacccgctcc
    10921 tgccggacgt gccgaccgcc agggagctcg gcgtcgacgt ggtggcccgc aacaaccgca
    10981 tctggctcgg ccccaagggc atgccggacg aggtcgtggc gcacctgaac aacgccttcc
    11041 gcacggcgat ggccgacccg gcgatccagg agcagatggt cgaccagggc ctcgtgccgg
    11101 tcttcgtcga gccggatgcc ctgctcgagg agctcgactg gtgggccgag aaggcggggc
    11161 cgctcgtcgc caaggcgcgc gagctgcagc agtaggcagg gctttcgcgc atggaccttc
    11221 ggcgccgtct ctgggtcgac gcggcggggc ggcgggatct gctgctcgcc ctcgccgtga
    11281 tcctgctcgc cctggtggtc tactggcagg ccagcctgct gccgccaccc ttcttcgatc
    11341 ccgtcggctc ggcggcggtg ccgaaattcg tggcgctcct gctggcgctg ctcgccgtcg
    11401 gcgtcatcgt cgagcggctg cgggcgaagc cgggcgcggt cgaggcgggc ttcgcgcccg
    11461 aggtgatcga cgacgacccg gccgagcccg caccctggac cgccctcgcc agcatcgtcc
    11521 tgccggttct ctatgtggcg gtcatgcagg cgggctggct cgccttcgcc caggcctcga
    11581 ccctcttcgt cctggcgctc ggggccctct tcggccgctt ccggccccgg ctcctgctgc
    11641 tgctggtgcc ggtcgcgttc ctcgccggct acggcctcga ctacatcttc acggagatcc
    11701 tctacgtgga cctgccgcaa cgctcgctgc tcggaggctc ctgagccgtg ctcggcgatc
    11761 tggggtcggc cctcctctac atcgcccagc cctggccgtt cttcttcgtg ctgctcggca
    11821 cctcgctcgg cgtcttcgtc ggggccgtgc ccggcctgac cggcgggatg ctgatagcac
    11881 tggtcatgcc cttgaccttc tacatggaca gcaccctggc gctggtcatg atggtggcga
    11941 tgtatgtggg ctcggtctcg ggcgggctga tcagcgccac gctgctccgc atgccgggca
    12001 cgccgtcctc ggtgatgacc accttcgacg gctatcccat ggcccagcag ggccagccgg
    12061 agcgggcgct ggcgctcggc atcggctcgt cgctgatcgg cgggctgatc gccggcgtcg
    12121 tcctcgtgct gctctcgccg ccgctctcca agtgggccat gaccttcagc ccctgggagt
    12181 atttcaccat ggtgctgatg gcgatcgtgc tgatcgcctc gatcagccag ggcagcatga
    12241 tcaagggcct gatcgccggc ttcctcggca tggtcatggc catgcccggc ctcaacccga
    12301 gcgacggcca gctccgcctc accttcggca tcgacgcctt cgccgacggc ttcaagctgc
    12361 tgcccgtgct gctcggcgcc ttcgtcatga gccaggtgat caaggacgcg ctcgagatca
    12421 acaagcgccc ggtgatgctg aagctcggcg ccggccgcgt cctcgtgccc ttgcgggaat
    12481 ggaggagcca ctgggtcaac atcctgcgct cgtcggtgat cggcacctgg atcggcatcc
    12541 tgcccggtgt gggcgcctcg atctcggcga tggtcgccta cgtgaccgcg aagaacctct
    12601 cgaggacgcc ggagaagttc ggcaccggcc atgccgaggg catcgtcgcc tcggaggccg
    12661 ccaacaacgc caatgtcggc ggggcgctga tcccgctgat caccatgggc atcccgggca
    12721 gccccatcga cgcgatcctg ctctcggccc tgatcctcca caacatccag ccgggcccgc
    12781 tgctcttcat caccaatggc gagttcgtct gggcgctgat ggcggcctac ctcgtcgcca
    12841 acgtcgtgat gttcctcctc atgaccctct cgatccgctg gatcgcgcgg gtggtgacgg
    12901 tggagaaggc attcatcctg ccgatcatct tcgtctgctg cgtgatcggc gcctatgcgc
    12961 tctccaaccg gatgtacgat gtctgggtgg tgatcgcctt cggcgtcatc ggcttcctgc
    13021 tcgaccgggc caaggtgccg ctcggcccct tcgtcatcgg cttcgtgctc gccggcatct
    13081 tcgaggcgga gctgcgctcc agcctgcaga tgtcggacaa cggcatcctc ggcatcctcg
    13141 agcggccgat cgccatgacc ttcgtcctcg tctccgtcgt catgctggtc tggcccttcg
    13201 tccagagctg gcgccagcga cggtccgcca ctgcctcgga gactacccga tgacgccgtc
    13261 caacgttctc ttcatcatgt cggacgagca cagccggcgg catctgggcg cctatgacga
    13321 cacgttcgtg cggaccccga gcatggatgc gctggcggcg cagggcacgc tcttcgagaa
    13381 cgcctattgc aactgcccga tctgcgtgcc gtcgcgggcg agcttcgcca ccggccgcca
    13441 tgtccacgag atcggcaact gggacaatgc ctcgccctac cagggggagc ctccttcctt
    13501 cggccacacg ctggccgcca cgggccatcg ctgcgattcg atcggcaagc tgcactaccg
    13561 cagtgcggag gacccgaacg gcttcgacaa cgagatcctg cccttgcacg tcttgaacgg
    13621 gaagggcgac gtgcagggca tcctcaggag cccgccgccc gtgcgggaag cgaccaagca
    13681 gctcgccgcc gacgccggcg tgggcgacag cacctatctc gactacgacc gcaagatcag
    13741 ggatgccgcc tgcgagtggc tggaagagac ggcgaaagcg ccgccggaca agccctggat
    13801 gctcttcgtc tccttcgtct gcccgcattt cccgctggtg gcgccgaagg agttcttcga
    13861 tctctacccg ctcgaccagg tgccgatgcc gcgcctcacc gggccggacg agctcccgga
    13921 ccacccggtg ctgcgcatgt tccgcaaggt gcagaactac caggaccatt tccgcgacga
    13981 ggcgcacgtg cggaccgcga ttgccgccta tctcggcatg gtgagcttcc tcgacgacaa
    14041 tatcggccgg ctgttgcgca ccctggacgc gaccggcctc ggccgcgaca cggtcgtcgt
    14101 ctacacttcc gaccatggcg acaatctcgg cacccggacg ttctggggca aatccaacat
    14161 gtacgacgag agcgccggcg tgccgctgat catggccggg ccgggcgtcc ctgtgggcag
    14221 gcgggtcaag acgccggtct cgctggtcga tgcgcacccg accatcctcg aggtcatggg
    14281 cgaggcttcc gacccggcgc tgcccggccg ctcgctgctg gccatcgccg agggtgcgga
    14341 gcccgaccgg acggtcttcg ccgagtatca tgcggtggcc tcgatcaccg gcatcttcat
    14401 ggtccgcttc ggccgctaca agcatatcca ctac
//
LOCUS       DAOTFI010000124        14124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5178_length_14124_cov_6.897576, whole genome shotgun sequence.
ACCESSION   DAOTFI010000124 DAOTFI010000000
VERSION     DAOTFI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5178_length_14124_cov_6.897576"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="ftsY"
                     /locus_tag="P5558_13555"
     CDS             <1..879
                     /gene="ftsY"
                     /locus_tag="P5558_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRY25384.1"
                     /translation="GSIAGIFTKRRLDDETLEELEEALIMADLGVATSAKMVQAIAKG
                     RFGKEVSDVEIRTALAAEIGRVLAPVAEPLPMREALRPQVILVCGVNGVGKTTTIGKL
                     AKQQMLAGRRVLMAACDTFRAAAVEQLEIWGSRNGCPVMKARTGADAAGLAYDALARA
                     RREGFDVLLIDTAGRLQNKAELMDELAKIVRVIRKLEPTAPHDVLLVLDATTGQNVHN
                     QVEIFQAMVEVSGLVVTKLDGSAKGGVVVALAERFGLPVHAVGVGEGIDDLRPFSADA
                     FARGLLGLEPAARDAA"
     gene            913..1377
                     /locus_tag="P5558_13560"
     CDS             913..1377
                     /locus_tag="P5558_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRY25385.1"
                     /translation="MKRNVVESLLGALVLLVAVGFFVWAYARADVGDPGGNVLSAKFD
                     RVDGLDVGGAVRISGIRVGQVLEQRLDPVSFRAEVRFSVERSVELPADSTAAVVSSGL
                     LGGKYLAIVPGGDDVILADGDEITFTQSSVNFEDLLGQFIFSQTSGGGAATE"
     gene            1374..1796
                     /locus_tag="P5558_13565"
     CDS             1374..1796
                     /locus_tag="P5558_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HRY25386.1"
                     /translation="MRHGLGLAAALLLASPGAATAGPALEHGVAVLQGLDKVTARVSV
                     LELRIGEEIRFGSLAIRARACLETPPTEPPESAAFLEIDEVEAGDGPEAVFTGWMFAS
                     TPGISALEHPVYDVWVIDCAEPLAGDEPGTPTPAESPQ"
     gene            complement(1790..2293)
                     /locus_tag="P5558_13570"
     CDS             complement(1790..2293)
                     /locus_tag="P5558_13570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRY25387.1"
                     /translation="MNATPEAFVAAMRLLPAGVTIVTSGPVGRRNGFTATAVCSVAAD
                     PPHLLVCANRGMETHGTILETGHFAVNVLSLGQEALADRFGGRLGIDGEARFQAADWH
                     TLASGAPILVSACAAFDCRVTQRLTVATHDVIIGRVLAATDREAAAPLAYLDRDYAFC
                     VPAGGRY"
     gene            complement(2346..3800)
                     /locus_tag="P5558_13575"
     CDS             complement(2346..3800)
                     /locus_tag="P5558_13575"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRY25388.1"
                     /translation="MQLILDHDKDVRRPKIERRADWLAPSVVTGMLRVGDVLLVLLSG
                     LGAYVFRFGTTEIDTFTTYSLAIGALVSLNAFQLAGLYQFQHLTQLAEQARRLLLAWP
                     LVLGLLLLLGFAAGLLDTVSRGWVALWLLTGFVALFGLRCLTRMQLLRWARAGRLTRN
                     IAVIGAGEHGRRFIEHLRRQQRHVNIIGLFDDRRDRIPDYIAGYPVLGTIDDLLVFAR
                     QHRIDQVVVALPWGAEERLLGWLKKLKSLPADIRLCPDVIGFHLPHRGVSHIGGVPLL
                     NVFEKPIAGWDGIVKSVEDRVLACLILLLVSPLMLSIALGVKLSSPGPVFFRQKRYGF
                     NNEIIEVFKFRSMYTDHAQAGLGTVEQARRTDPRITPFGAILRRTSLDELPQFLNVLR
                     GEMSIVGPRPHAVAHNEQYAQLIDEYLARHRVKPGITGWAQVNGLRGETETLEKMERR
                     VQFDLHYIENWSLALDLRIILMTLLVGFSHPNAY"
     gene            complement(3958..4683)
                     /locus_tag="P5558_13580"
     CDS             complement(3958..4683)
                     /locus_tag="P5558_13580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY25389.1"
                     /translation="MSGPSQATGHRSTKDVIREALRPVGRQILDIGSGAGALVAWLRR
                     EGALAIGIEPNAPLLQAAFARSSAGRGASPNDRWIAARAEELPLADGSVDAALFFNSL
                     HHVDPAAQGAALAEAARVLRPGGDLLVIEPLASGSYFELLRPLDDETAVRAAALAAID
                     AIPDGLLVATLRLQYMTFLDYADVAATVAAFTRADPARAAAVEQALPAIAERFARLGR
                     PGADGSRRFEQPMLAVSLRRLCA"
     gene            4734..4961
                     /locus_tag="P5558_13585"
     CDS             4734..4961
                     /locus_tag="P5558_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25390.1"
                     /translation="MRGTILVIVVLLGLLAFASVLAYDTWQDMSETQISFHGMVALGL
                     GVGLTLLLGIGLMSLVYFSHRRGYDDEAGRD"
     gene            complement(4934..5458)
                     /locus_tag="P5558_13590"
     CDS             complement(4934..5458)
                     /locus_tag="P5558_13590"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY25391.1"
                     /translation="MIFVGVGTFAHGFDELVAGADRAAAALGIEGFAQIGHSRVVPRH
                     LDHARFLGSDELAARLGRSEVAVCHAGMGLVGDALRAGCRIVLVPRQGSISRRHPAND
                     QRRFAERLAAILPLRVCTDPADLAGAIRAALDDAGPPPAAPPSTVPAILQDYLRTGAR
                     GAAPPPQSRPASSS"
     gene            complement(5455..5943)
                     /locus_tag="P5558_13595"
     CDS             complement(5455..5943)
                     /locus_tag="P5558_13595"
                     /inference="COORDINATES: protein motif:HMM:NF020244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25392.1"
                     /translation="MSGAPADTIHVERRRVCLAYSPGGHKAELDRALAGIRFTDVCHV
                     TFDDGRPAPPLEAGVRRHVVCHPRRSLGRTLLNAWQSLAVLRRERPALVISTGADVAV
                     PLLLLAKCTGAKVIFIETAGSGEPTLAGRLVYPFADLFVVQWPDRLRRFPKAVLAGGL
                     LL"
     gene            complement(5940..6620)
                     /locus_tag="P5558_13600"
     CDS             complement(5940..6620)
                     /locus_tag="P5558_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25393.1"
                     /translation="MQPKAKTFRFRASSTEPWFATHSCLVILAVNWLAQGMRGMDPKE
                     LSFRLLLLCGLTGLLAAVLVAGPVAAPGPALLLGLGLAHSINFTLNGQLWVCLRYCPA
                     YRQDPVVLATRTGALVRRVRRSTWLQEAVLIGSAARLRSLPGPRSDIDLRLIFPPGWS
                     GWLRTNLLLLELRARALVVGLPLDLYAYDHPAQLRRFEQTEPLALVLDRQARLCRGFA
                     NRELVRIP"
     gene            6739..7974
                     /locus_tag="P5558_13605"
     CDS             6739..7974
                     /locus_tag="P5558_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25394.1"
                     /translation="MAFLATLVMPALSASTIAALGFADLRIIVFVCLFALLAMGAILR
                     RPRLDRRQRTVPLVALWLLGLIAAVGQGQAGELLDYKILLPILALLVAPNLRAALGDL
                     AVVRVVLLAGGLYVLATAAGAMLVPATATLRNVAEHVRIDLTGSVVLHASLCTIVAVA
                     AFAACAACRRPWRRLVLALVVIAAACMVLLTGTRTALVTLFILAVLAILSGAWQPLAR
                     PRALGLGLAALGGLVLLSTLLGDTLWARLVELDRADYSSGRWPSIGHWLALSGGAPFG
                     LGMGTIRATLAAGRPLIGDGQPLEWPHNELVRFYVEAGVPGVVLVLLLLGEVTRLALR
                     RARLTGDPAERALVLVIAADLWAQCLFQNYFNGVYQATVLMLLVGVLAAGGAAGRVPR
                     PPATAPAGAAGWPTMRPTA"
     gene            7991..8605
                     /locus_tag="P5558_13610"
     CDS             7991..8605
                     /locus_tag="P5558_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25395.1"
                     /translation="MPFPSAGRRPTGVRPGRGRQVPAFGAGVRRTVAGLEINTIPPND
                     YSYYEMLNDLMQAEPATSLDPELMGPIAAIGIVKGKEFAPDERMKRILTDAVAVANAT
                     GRTLGFDPVDPDWFYYESSQWWKPLFLGGYLFETPLPEITKEGARPFPPTGYRQNSAR
                     TSFFYMATGITPAMAMRLTGIGSQYLFAARDADGSFFDGAKTTR"
     gene            8602..8925
                     /locus_tag="P5558_13615"
     CDS             8602..8925
                     /locus_tag="P5558_13615"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRY25396.1"
                     /translation="MTLPKDVPAGAFWSFTLYDNQTRSMLVTPQEYPRAGSQSYPSPA
                     AEAGEDGSTTVWFAPEQPDGVGRGNRIQTVPGKGWVTILRLYSPLPPFFDKSRRPGEI
                     EPVRE"
     gene            9076..10074
                     /gene="dctP"
                     /locus_tag="P5558_13620"
     CDS             9076..10074
                     /gene="dctP"
                     /locus_tag="P5558_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY25397.1"
                     /translation="MIRKLGTLGIAAALAAALVSTAGAQTTLRASHQWPGGKGDIRDE
                     MVQIIKREVEAADVGLEIQVFPGASLFKANEQWGALTSGQLDLAAFPLDYASGRVPQF
                     SATLMPGLVRNHERAQRLNDSPFMDEIKRLIEENRVVVIADAWLAGGFASKKNCITGP
                     ESIAGQVTRAAGPAFEQMLAAAGASIASMPSSEVYTGMQTGVLDAANTSSGSFISYRL
                     YEQVKCLTAPGENALWFMYEPILMSQRSWENLNEEQQAALMAAGEVAEAYFFEEAKKV
                     DQEMIDVYEKAGVEVVEMTPEDYDAWLALAEDSSYKDFAANVPGGAELIQLALDVE"
     gene            10163..10687
                     /locus_tag="P5558_13625"
     CDS             10163..10687
                     /locus_tag="P5558_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY25398.1"
                     /translation="MRLYIVTVTALSRAGGVLAMLLLAAAVLVTCQMIFVRYLLGGST
                     IWQTEFVIYSIVAATFIGSPSVLIDRGHVGVDLLPKALGGTGRRWLEVIGGVAALAFC
                     LLLAWSGFIYFYEALTRGWRTATVWQLPLWIPLFPLPLGIGLLALQYVAEMLKLRDGG
                     TLLAGEATHFEELE"
     gene            10687..12003
                     /locus_tag="P5558_13630"
     CDS             10687..12003
                     /locus_tag="P5558_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY25399.1"
                     /translation="MGPFASGSLLLLGLLVLLATGMPIAFALGLVSIVALILSGGFGV
                     MEIIGETFFGGLASFSLVAIPMFILMGSAVASSPAGRDLYEALDRWLNRVPGGLVLSN
                     IGACSIFSALSGSSPATCAAIGKMGIPAMVERGYPASIATGSIAAGGTLGILIPPSVT
                     MIVYGISTETSIGRLFLAGVLPGVMLTALFMIWTIISCRIAGYSFEAMARRFTLREKL
                     QALPRVLPFLSIVVAILYVLYGGVATPSEAAGAGALFCIVLAVVIYRMWRPGPLVEIF
                     RSTLRESVMIMMIIGASELFAFALSSLFVTQSIAMWIADLEVNRWVLLLFVNIFLLIA
                     GFFLPPVAVILMTAPILLPIIVQAGFDPYWFAVVLTINMEIGLITPPVGLNLYVINGI
                     APDIPLQTILKGALPYVLCMMLGIIILCFFPGIATWLPGRMMGVAL"
     gene            12000..13376
                     /locus_tag="P5558_13635"
     CDS             12000..13376
                     /locus_tag="P5558_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HRY25400.1"
                     /translation="MNTYFFNDLLGTIVERGRSIIERGQGRAATDDDLADLAETLVGG
                     RGEASGVAVAATLLDGYAALDREARVGFFEILAERFGAPPEPLAAAARAFLEQPDDTH
                     ALRLHDVSESRRQELIRRLNRAPGGTAALVAMRADLLRELRERPELAAVDHDFRHLLS
                     SWFNRGFLVLRRIDWSTPAVVLEKVIRYEAVHRINGWEDLRRRIDPADRRLYAFFHPA
                     LVDDPLIFVEVALTGDIPSAIGPILAEERQPLDPKRASTAVFYSISNCQEGLRGISFG
                     HFLIKQVVEELARDFPALDTFVTLSPVPGFRAWLDQERGKEGPAVLTAADRALLDELD
                     DPGWTADEATVAAARPVLLALGASYFHQSLTKHGTPTDPVARFHLGNGARLERVNFLA
                     DGSPKALAASHGLMVNYLYDPRQIERHHEAYANEGKIAVSPAVRRLVRKTSQREPPQP
                     ESAIASHE"
     gene            13369..>14124
                     /locus_tag="P5558_13640"
     CDS             13369..>14124
                     /locus_tag="P5558_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY25401.1"
                     /translation="MSDDLYSALEAAIPDPRKALFALADGSEVSYGETFALAGRIANL
                     FKARGVVPGDRVAVQVDKPWQAVAVFLASLRAGAVYLPLNAGYTPTEIAYFLGDAEAR
                     LFVCRPESLATLAPVAREAGVAALETLGNDGSGSLLEAAALESDAFATVPRAADDLAA
                     ILYTSGTTGRSKGAMLTQRNLLSNAATLAEFWRFTDRDVLIHALPIFHTHGLFVAIDT
                     VMLSGGTMIWRARFDADEVIGLMGRATSLMGVPT"
BASE COUNT         2021 a         4797 c         5117 g         2189 t
ORIGIN      
        1 ggcagcattg ccgggatctt caccaagcgg cggctggacg acgagacgct ggaggagctc
       61 gaggaggcgc tgatcatggc ggacctcggg gtcgccacct ccgcgaagat ggtccaggcg
      121 atcgccaaag ggcggttcgg caaggaggtc tcggacgtcg agatccggac ggcgctggcg
      181 gccgagatcg gccgggtgct ggcgccggtg gccgagccgt tgccgatgcg cgaggcgctg
      241 cggccgcagg tgatcctggt ctgcggggtg aacggggtcg gcaagacgac gaccatcggc
      301 aagctcgcca agcagcagat gctggccggc cggcgggtgc tcatggcggc ctgcgacacg
      361 tttcgcgcgg cggcggtcga gcagctcgag atctggggca gccgcaacgg ctgcccggtg
      421 atgaaggcca ggaccggcgc cgatgcggcg gggctcgcct atgacgcgct ggcgcgcgcc
      481 cggcgcgagg gcttcgacgt gctgctgatc gacacggccg ggcggctgca gaacaaggcg
      541 gagctgatgg acgagctcgc caagatcgtc cgggtgatca ggaagctcga gcccaccgcc
      601 ccgcatgacg tgctgctcgt gctcgacgcc acgaccggcc agaacgtcca caaccaggtc
      661 gagatcttcc aggccatggt cgaggtcagc ggcctcgtcg tcaccaagtt ggacggctcg
      721 gccaagggcg gggtggtggt ggcccttgcc gagcgcttcg gtttgccggt acacgcggtc
      781 ggtgtcggcg aggggatcga cgatctcagg ccgttctcgg ccgacgcctt cgcccgcggc
      841 ctgctcggcc tcgagccggc cgcgcgggac gcggcctgac cgaccccgcc acgcagccgg
      901 agacctgtgc ccatgaaacg caatgtcgtc gagagcctgc tcggagccct ggtcctcctg
      961 gtggccgtgg gcttcttcgt ctgggcctat gcgcgtgccg atgtcggcga tcccggcggc
     1021 aacgtgctga gcgccaagtt cgaccgggtc gacgggctcg atgtgggcgg ggccgtgcgg
     1081 atcagcggca tccgggtggg ccaggtgctc gagcagcgcc tggacccggt ctcgttccgg
     1141 gccgaggtgc ggttcagcgt cgagcgatcg gtcgagctgc cggccgacag cacggcggcg
     1201 gtggtcagct ccggcctgct cggcggcaag tacctggcga tcgtgccggg cggcgacgac
     1261 gtcatcctcg ccgatggcga cgagatcacc ttcacccagt cctcggtgaa cttcgaggac
     1321 ctgctcggcc agttcatctt cagccagacc agcggcggcg gcgccgccac ggaatgaggc
     1381 acgggctcgg tctcgccgct gccctgctcc tcgcctcgcc gggtgcggcc acagccgggc
     1441 ccgcgctcga gcacggcgtg gcggtgctgc agggcctcga caaggtgacc gcccgggtca
     1501 gcgtgctgga gcttcggatc ggcgaggaga tccgcttcgg cagcctcgcc atccgggcgc
     1561 gggcctgcct cgagacgccg ccgaccgagc cgccggaaag cgcggccttc ctcgagatcg
     1621 acgaggtcga ggccggcgac gggcccgagg cggtcttcac cggctggatg ttcgcctcga
     1681 cgccgggcat ctccgccctc gagcatccgg tctacgacgt ctgggtgatc gactgcgccg
     1741 aaccgctcgc cggggacgaa cccggcaccc cgacgcccgc cgaatctcct cagtagcggc
     1801 cgccggccgg cacgcagaac gcataatcgc gatcgagata ggcgaggggg gccgccgcct
     1861 cgcgatcggt cgccgccagc acgcggccga tgatgacatc gtgcgtggcg acggtgaggc
     1921 gctgcgtgac ccggcagtcg aaggcggcgc aggcgctcac caggatgggg gcaccgctcg
     1981 ccagggtgtg ccagtcggcg gcctggaacc gggcctcgcc gtcgatgccg aggcgcccgc
     2041 cgaagcggtc ggccagcgcc tcctggccaa gggaaagcac gttgacggcg aaatgaccgg
     2101 tctcgaggat ggtgccgtgg gtctccatgc cgcgattggc gcagacgagg aggtgcggcg
     2161 ggtcggccgc cacggagcag acggcggtgg cggtgaagcc gttgcgtcgg ccgacggggc
     2221 cgctggtcac gatggtgacg cccgccggca ggaggcgcat ggcggcgacg aaggcttcag
     2281 gcgtggcatt cacgaacgac gacgcttccc gggctcggcg ggacggcgcg tcccggtcgt
     2341 gctcgtcagt aggcgttcgg gtggctgaag ccgacgagca gggtcatcag gatgatacgg
     2401 agatcgaggg ccagcgacca gttctcgatg tagtgcaggt cgaactggac ccgccgctcc
     2461 atcttttcca gtgtctcggt ctcgccgcgc agcccgttga cctgcgccca gccggtgatg
     2521 ccgggcttga cccggtgacg ggcgaggtac tcgtcgatga gctgggcgta ctgctcgttg
     2581 tgggcgaccg cgtgcggccg cggcccgacg atcgacatct cgccgcgcag cacgttgagg
     2641 aactgcggca gctcgtcgag gctggtccgc cggaggatgg cgccgaaggg cgtgatgcgc
     2701 ggatccgtgc gccgcgcctg ctcgaccgtg ccgagcccgg cctgggcgtg gtccgtgtac
     2761 atcgagcgga acttgaagac ctcgatgatc tcgttgttga agccgtagcg cttctggcgg
     2821 aagaacaccg gccccggcga ggagagcttc acgccgaggg cgatgctcag catgagcggc
     2881 gagacgagga gcaggatgag gcaggcgagg acgcggtcct cgaccgactt gacgatgccg
     2941 tcccagccgg cgatcggctt ctcgaagacg ttgagcagcg gcacgccgcc gatatgcgac
     3001 acgccccgat gcggcagatg gaagccgatg acgtccgggc agaggcggat gtcggccggc
     3061 aggctcttca gcttcttgag ccagccgagc aggcgctcct ccgcccccca gggcaacgcc
     3121 acgacgacct ggtcgatgcg gtgctggcgg gcgaagacca gcaggtcgtc gatcgtgccg
     3181 agcaccgggt agcccgcgat gtagtccggg atgcggtccc gccgatcgtc gaagaggccg
     3241 atgatgttga cgtgccgctg ctggcggcgg agatgctcga tgaagcggcg cccgtgctcg
     3301 ccggcgccga tgaccgcgat gttgcgggtc agccgaccgg ccctcgccca gcgcaggagc
     3361 tgcatccggg tgaggcagcg caggccgaag agcgccacga agccggtgag cagccagagc
     3421 gccacccagc cgcgcgagac cgtgtcgagg aggcctgcgg cgaaccccag gagcaggagc
     3481 aggccgagca ccaggggcca tgccaggagc aggcggcggg cctgctcggc gagctgggtc
     3541 agatgctgga actggtagag gccggcgagc tggaacgcgt tgagcgagac cagggcaccg
     3601 atcgccaggc tgtaggtggt gaaggtgtcg atctcggtcg tgccgaagcg gaagacatag
     3661 gcgccgaggc cgctgagcag gacgagcagc acgtcaccga cgcgcagcat gccggtcacc
     3721 acgctcggtg ccagccagtc ggcgcgccgc tcgatcttcg ggcggcggac gtccttgtcg
     3781 tgatcgagga tgagttgcac gggattctgc cgcttcattc cggccgactg ctgtaaggcc
     3841 ggggcccgcc gtcagcggcc cctgacactc ttcttgccac gcaatcctta accgggcgtt
     3901 aagctgcccg ctgaaggtgt atcacctaac ggtagaatta tctctctgaa attcgcctta
     3961 tgcgcacaat cggcgaagcg agacggccag catcggctgc tcgaagcgcc ggctgccgtc
     4021 ggccccgggt cggcccagcc tggcgaaacg ctcggcgatc gccggcagcg cctgctcgac
     4081 ggcggcagca cgggcgggat cggcgcgggt gaaggcggcg acggtggccg ccacgtccgc
     4141 gtagtcgagg aacgtcatat actggaggcg cagggtcgcc acgagcaggc cgtcggggat
     4201 ggcatcgatg gcggcgagcg ccgccgcgcg caccgccgtc tcgtcgtcga gcggtcgcag
     4261 cagctcgaaa tagctgcccg aggcgagcgg ctcgatgacc aggagatcgc cacccggccg
     4321 gagcacgcgc gccgcctcgg cgagggccgc gccctgtgcc gccgggtcga cgtggtgcag
     4381 gctgttgaag aagagggccg catccacgct gccgtcggcg agcggcagct cctcggcccg
     4441 ggccgcgatc caacggtcgt tcgggctcgc tccccggccg gccgacgagc gcgcgaaggc
     4501 ggcctgcagc agcggggcgt tcggctcgat gccgatcgcg agggcaccct cgcggcggag
     4561 ccaggcgacg agggcgccgg caccactgcc gatgtcgagg atctgccggc ccacgggccg
     4621 gagcgcctcg cggatgacgt ccttcgtgct tcggtggccg gtcgcttggg atgggcccga
     4681 caccttgcta tgctcccggc cgattgtcgg ccccgaccct ggagacccgg atcttgcgcg
     4741 gcaccatcct cgtcatcgtc gtgctcctcg gcctgctcgc cttcgccagc gtgctggctt
     4801 acgacacctg gcaggacatg agcgagacgc aaatcagctt tcacggcatg gtggccctcg
     4861 gcctcggtgt cgggctgacc ctgctgctcg ggatcggcct gatgagcctc gtctatttca
     4921 gccaccggcg cggctacgac gacgaggccg ggcgcgactg aggcggtggc gccgcgcccc
     4981 tggcgccggt gcggagatag tcctggagga tcgcgggcac ggtcgagggc ggtgcagcgg
     5041 gcggtggccc cgcgtcatcg agcgccgccc ggattgcacc cgcgagatcg gccggatcgg
     5101 tgcagacgcg cagtggcagg atcgccgcga ggcgctcggc gaagcggcgc tggtcgttgg
     5161 ccggatggcg ccggctgatg ctgccctggc gcggcaccag cacgatgcgg cagccggcgc
     5221 ggagcgcgtc gccgaccagg cccatcccgg catggcagac ggcgacctcg ctccggccga
     5281 gccgtgccgc cagctcgtcc gacccgagaa agcgcgcatg atccaggtgg cggggcacca
     5341 cccgcgaatg gccgatctgg gcgaagccct cgatgccgag cgctgcagcg gcccggtcgg
     5401 cgccggcgac cagctcgtcg aagccgtggg cgaaggtgcc gacgccgacg aagatcacag
     5461 gagcaggccc ccggccagca cggccttcgg gaagcggcgc aggcggtcgg gccactggac
     5521 gacgaagaga tcggcgaagg ggtagacgag gcggccggcc agcgtcggct cgccgctgcc
     5581 cgcggtctcg atgaagatca ctttggcacc cgtgcatttg gccagcagca gcagcggcac
     5641 ggcgacgtcg gcgccggtcg agatgaccag ggccggccgc tcccgacgga ggacggcgag
     5701 gctctgccag gcattcagga gcgtgcggcc gagcgagcgg cgcgggtggc agacgacgtg
     5761 gcggcggacg cccgcctcga gtggcggggc agggcggccg tcgtcgaagg tgacgtggca
     5821 gacatcggtg aagcggatgc cggcgagggc gcggtcgagc tccgccttgt ggccgcccgg
     5881 cgagtaggcg aggcagacgc ggcgccgctc gacgtggatc gtgtcggcgg gcgccccgct
     5941 cacgggatgc ggaccagctc gcgattggcg aagccgcggc agaggcgcgc ctgccggtcg
     6001 aggacgaggg cgagcggctc ggtctgctcg aagcggcgca gctgcgccgg atggtcgtag
     6061 gcgtagaggt cgagcggcag gccgacgacg agcgcccggg cgcgcagctc caggagcagg
     6121 aggttggtgc gcagccagcc gctccagccg ggcgggaaga tcagccggag atcgatgtcc
     6181 gagcgcggtc cggggagcga gcgcaggcgc gccgccgagc cgatcagcac cgcctcctgc
     6241 agccatgtgc tgcggcgcac gcgccggacc agcgccccgg tccgggtcgc gagcaccacc
     6301 gggtcctggc ggtaggccgg gcagtagcgc aggcagaccc agagctggcc gttcaacgtg
     6361 aagttgatcg aatgcgccag gccgaggccg agcagcagcg ccgggccggg tgcggccacg
     6421 gggccggcga cgagcacggc ggcgagcagg cccgtgagcc cgcagagcag cagcaggcga
     6481 aagctcagct ccttgggatc catgccgcgc atgccctggg ccagccagtt gaccgcgagg
     6541 atgacgaggc agctgtgcgt ggcgaaccag ggctcggtcg agctcgcgcg aaagcggaag
     6601 gtcttggctt tgggttgcat cggcaggttg cgcaggcacc ggctgtatcg ctagaatatc
     6661 aacttataat tgaaatcgcg catggaggac aagatgcacg ttggccggtc ggggggggcc
     6721 gctagcggtg ccgtccgctt ggccttcctg gccacgctgg tcatgccggc gctctccgcc
     6781 tcgaccatcg cggcgctggg cttcgccgat ctgcggatca tcgtcttcgt ctgtctcttc
     6841 gcgcttctgg ccatgggcgc cattctgcgc cggccccgtc tcgaccggcg ccagcggacg
     6901 gtgccgctgg tcgcgctctg gctcctcggg ctgatcgcgg cggtgggtca gggccaggcc
     6961 ggcgagctgc tcgactacaa gatcctgctg ccgatcctgg cgctgctggt ggcgcccaac
     7021 ctgcgcgcgg cgctcggcga cctggcggtc gtgcgggtcg tgctgctggc gggcgggctc
     7081 tacgtgctgg cgacggcggc gggggcgatg ctggtgcccg cgaccgccac gctccgcaac
     7141 gtcgccgagc atgtgcgcat cgatctcacc ggctccgtcg tgctgcatgc cagcctctgc
     7201 acgatcgtcg ccgtggcggc gttcgccgcc tgcgccgcct gccgccggcc gtggcggcgc
     7261 ctcgtgctcg ccctcgtggt gatcgccgcc gcgtgcatgg tgctgctcac cggcacacgc
     7321 acggctctgg tcacgctttt catcctggcg gtcctcgcga ttctgtcggg cgcctggcag
     7381 ccgctggcgc ggccccgcgc gctcggcctc ggcctcgctg ccctgggcgg gctcgtgctg
     7441 ctctcgacgc tcctcggcga cacgctctgg gcccggctgg tcgagctcga tcgggcggac
     7501 tactcgtccg gccgctggcc gtcgatcggg cattggctgg cgctgagcgg tggtgccccc
     7561 ttcggcctcg gcatgggcac catacgcgcc acgctggccg ccggccggcc gctgatcggc
     7621 gacgggcagc cgctcgagtg gccgcacaac gagctcgtcc gcttctatgt cgaggcgggg
     7681 gtgccggggg tggtgctggt gctcctgctg ctcggcgagg tcacccgcct ggcgctcagg
     7741 cgggcacggc taacgggcga tccggccgag cgggcgctgg tcctggtgat cgccgccgat
     7801 ctctgggctc aatgcctgtt ccagaactat ttcaacgggg tgtaccaggc gaccgtgctg
     7861 atgctgctgg tcggcgttct ggcggcaggc ggtgccgccg ggcgcgttcc gcgtcccccg
     7921 gcgaccgcac cggcaggagc ggcgggatgg cccacgatgc gcccgacagc ttgatccgca
     7981 cgcccggccg atgccgtttc cgtctgccgg gcggcgtccg accggggtcc gaccggggcg
     8041 agggcgacag gtacccgctt ttggggccgg ggtacgaagg accgttgccg ggctggagat
     8101 caacacgatc ccgccgaacg actacagcta ctacgagatg ctgaacgacc tcatgcaggc
     8161 ggagccggcg acgagcctcg atcccgagct gatggggccg atcgccgcca tcggcatcgt
     8221 gaaaggcaag gaattcgcgc cggacgaacg gatgaagagg atcctgaccg acgccgtggc
     8281 agtcgccaac gcgaccggcc gcacgctcgg cttcgacccc gtggacccgg actggttcta
     8341 ctacgagagc tcgcagtggt ggaagccgct cttcctcggc ggctacctgt tcgagacgcc
     8401 gctccccgag atcacgaagg agggcgccag gccgttcccg ccgaccggct accggcagaa
     8461 cagcgcccgc acgtcgttct tctacatggc gacggggatc accccggcca tggcgatgcg
     8521 gctgaccggc atcgggtcgc aatacctgtt cgccgccagg gacgctgacg gcagcttctt
     8581 cgacggtgcg aagactacaa ggtgacgctg ccgaaggacg ttccggccgg ggcgttctgg
     8641 tccttcaccc tctacgacaa ccagacccgc tcgatgctgg tgaccccgca ggaatacccg
     8701 cgcgccggca gccagagcta cccctccccg gcggccgagg cgggtgagga cggctcgacc
     8761 acggtctggt tcgccccgga gcagcccgac ggcgtcgggc gcggcaaccg gatccagacc
     8821 gttcccggga agggctgggt cacgatcctg cgcctctaca gcccgctgcc gcccttcttc
     8881 gacaagtccc ggcggccggg cgagatcgag ccggttcggg aatagcccgg agcgggcggc
     8941 ctcgccgacg atcgcgcgag ggggctctcc ggcgaggagg atcggccggt cgcgacggcg
     9001 agaaccattg acctcgcgcc cggcccgtga ttgtcacgat gtgaaacgcg gcaatcagga
     9061 cagggagcaa gggccatgat caggaaactg ggaacgctgg gaatcgcggc ggcgctggcg
     9121 gcggcgctcg tgtcgacggc cggcgcgcag accacgctgc gcgcctcgca ccagtggccg
     9181 ggcggcaagg gggacatccg cgacgagatg gtgcagatca tcaagcgcga ggtggaggcg
     9241 gccgatgtgg gtctcgagat ccaggtcttc ccgggggcct cgctcttcaa ggcgaacgag
     9301 cagtgggggg ccttgaccag cggccagctc gatctggcgg catttccgct cgactacgcc
     9361 tcgggaaggg tgccgcagtt ctcggcgacc ttgatgccgg gcctcgtgcg caatcacgag
     9421 cgggcgcagc ggctcaacga ctcgcccttc atggacgaga tcaagcggct gatcgaggag
     9481 aaccgggtgg tggtgatcgc cgacgcctgg ctggccggcg gcttcgcctc gaaaaagaac
     9541 tgcatcaccg ggccggagag cattgcgggt caggtaacgc gggcggcggg gccggccttc
     9601 gagcagatgc tggccgcggc cggcgcctcg atcgcgtcga tgccgtcctc ggaggtctat
     9661 accggcatgc agaccggcgt gctggatgcc gcgaacacct cgtccggctc gttcatctcc
     9721 tatcgcctct acgagcaggt gaagtgcctg acggcacccg gcgagaacgc gctctggttc
     9781 atgtacgagc cgatcctcat gtcgcagcgg agctgggaga acctgaacga ggagcagcag
     9841 gcagcactca tggccgcggg cgaggtggcg gaggcgtatt tcttcgagga ggcgaagaag
     9901 gtcgatcagg agatgatcga cgtctacgag aaggccgggg tcgaggtggt cgagatgacg
     9961 cccgaggact acgacgcctg gctcgccctc gccgaggaca gctcctacaa ggacttcgcc
    10021 gccaacgtgc cgggcggcgc cgagctgatc cagctcgccc tcgacgtcga gtagcccttc
    10081 cagcgaagca cgccggccgc gggcggcccc ggccggaagg cggggtgccg ctccggccgg
    10141 tccggtccgg gaccgacctg ccatgcggct ctacatcgtg accgtcaccg ccctctcacg
    10201 ggccggcggc gtgctcgcca tgctgctcct cgcggcggcg gtgctcgtca cctgccagat
    10261 gatcttcgtc cgctacctgc tcggcggctc gaccatctgg cagaccgagt tcgtcatcta
    10321 ctcgatcgtc gccgcgacct tcatcggctc accctcggtg ctgatcgatc gtggccatgt
    10381 cggcgtcgat ctgctgccca aggcgctcgg cggcacgggg cgccgctggc tcgaggtcat
    10441 tggtggtgtc gcggcgctgg ccttctgcct gctgctcgcg tggtcgggct tcatctattt
    10501 ctatgaggcc ctgacccgtg gctggcggac cgcaacggta tggcagctgc cgctctggat
    10561 cccgctcttt cccctgccgc tcggcatcgg cctgctggcg ctgcaatacg tggcggagat
    10621 gctgaagctt cgagacggcg gcacgctgct cgcgggcgag gcgacgcatt tcgaggagct
    10681 cgagtgatgg ggcccttcgc ctcgggctcg ctcctgctgc tcggcctgct cgtgctgctc
    10741 gcgaccggca tgccgatcgc cttcgcgctc gggctggtct cgatcgtggc gctcatcctc
    10801 tccggcggct tcggcgtgat ggagatcatc ggcgagacct tcttcggcgg gctcgccagc
    10861 ttcagcctcg tcgccatccc gatgttcatc ctgatgggct cggccgtggc ctcgagcccg
    10921 gccgggcgcg atctctacga ggcgctggat cgctggctca accgggtgcc gggagggctc
    10981 gtgctctcca atatcggcgc ctgctcgatc ttctccgccc tttccggctc gagcccggcg
    11041 acctgcgccg ccatcggcaa gatgggcatc ccggcgatgg tcgagcgggg ctatccagcc
    11101 agcatcgcca cgggctcgat cgccgccggc ggcacgctcg gcatcctgat cccgccctcg
    11161 gtcacgatga tcgtctacgg catctcgacc gagacctcga tcggccggct cttcctcgcc
    11221 ggcgtgctgc cgggggtgat gctgacggcc ctcttcatga tctggacgat catctcctgc
    11281 aggatcgcgg gctacagctt cgaggcgatg gcccggcgct tcacgctgcg ggagaagctg
    11341 caggcgctgc cgcgtgtgct gcccttcctc tcgatcgtcg tggccatcct ctacgtgctc
    11401 tatggcggcg tcgcgacgcc gtcggaggcc gccggtgcgg gcgccctctt ctgcatcgtg
    11461 ctggcggtgg tcatctaccg gatgtggcgg cccgggccgc tggtcgagat cttccgctcc
    11521 accttgcgcg agtccgtcat gatcatgatg atcatcggcg cctcggagct cttcgccttc
    11581 gccctctcgt cgctcttcgt cacccagtcg atcgcgatgt ggatcgccga tctcgaggtc
    11641 aaccgctggg tgctgctgct cttcgtgaac atcttcctcc tgatcgccgg cttcttcctg
    11701 ccgcccgtgg cggtgatcct gatgaccgca cccatcctgc tgccgatcat cgtccaggcg
    11761 ggcttcgacc cctactggtt cgccgtggtg ctgacgatca acatggagat cggcctgatc
    11821 acgccgccgg tcgggctcaa tctctatgtc atcaacggga tagcgccgga catcccgctg
    11881 cagaccatcc tcaagggtgc cttgccctat gtcctttgca tgatgctcgg cattatcatc
    11941 ctctgcttct tcccgggcat cgccacctgg ctgcccggcc ggatgatggg ggtggccctt
    12001 tgaacaccta tttcttcaac gatctgctgg gaaccatcgt cgagcgcggc cggtcgatca
    12061 tcgagcgggg ccagggtcgt gcggcgacgg acgacgacct cgccgacctc gccgagacgc
    12121 tggtcggcgg gcgcggcgag gcttccggcg tcgccgtggc ggcgacgctg ctcgatggct
    12181 acgcggcgct cgaccgggag gcgcgcgtcg gcttcttcga gatcctggcc gagcgcttcg
    12241 gcgccccgcc cgagcccctg gcggcagcgg ccagggcctt cctcgagcag ccggacgaca
    12301 ctcatgcgct gcgcctgcac gacgtgtccg aatcgcgccg gcaggagctg atccgccggc
    12361 tgaaccgggc acccggcggc acggcggcgc tcgtcgcgat gcgtgccgat ctcctgcgcg
    12421 agctgcgcga gcgtccggag ctcgctgccg tcgatcacga tttccgccat ctgctctcgt
    12481 cctggttcaa tcgcggcttc ctcgtgctgc gccgcatcga ctggtcgacc ccggcggtcg
    12541 tgctcgagaa ggtcatccgc tacgaggccg tgcaccggat caatggctgg gaggatcttc
    12601 ggcggcgcat cgacccggcg gatcgaaggc tctacgcctt cttccatccg gccctggtcg
    12661 acgacccgct catcttcgtc gaggtggcgc tgaccgggga cataccctcg gccatcggcc
    12721 cgatcctcgc cgaggagcgc cagccgctcg acccgaagcg ggcctcgacc gcggtcttct
    12781 actcgatctc caactgccag gagggcctgc gtggcatctc cttcggccat ttcctcatca
    12841 agcaggtggt cgaggagctg gcacgcgact ttccggcgct cgacaccttc gtcaccctct
    12901 cgccggtgcc gggctttcgg gcctggctcg accaggagcg cggcaaggag ggcccggccg
    12961 tgctgactgc cgccgaccgg gcgctgctcg acgagctgga cgatccgggc tggacagcgg
    13021 acgaggccac ggtggctgcg gcccggccgg tgctcctggc gctgggggcg agctatttcc
    13081 accagtcgct gacgaagcac ggcacgccga ccgacccggt ggcgcgcttc cacctgggca
    13141 acggggcgcg gctggagcgg gtcaatttcc tcgccgacgg ctcgcccaag gcgctcgcgg
    13201 cgtcgcacgg gctgatggtg aactatctct acgatccaag gcagatcgag cgtcatcacg
    13261 aggcttatgc gaacgagggc aagattgccg tctcgccggc cgtgcggcgc cttgtccgca
    13321 agacttccca acgcgagccc ccccaacccg agtcagcgat tgccagtcat gagtgacgat
    13381 ctctacagcg ccctcgaggc ggcgattccc gacccgagga aggcgctctt cgcgctcgcc
    13441 gacggcagcg aggtcagcta cggcgagacg ttcgcgctgg ccgggcggat cgccaatctc
    13501 ttcaaggcgc ggggcgtcgt gccgggcgac cgggtcgccg tgcaggtcga caagccatgg
    13561 caggcggtcg ccgtctttct cgccagcttg cgcgccgggg cggtctatct gccgctcaat
    13621 gccggctaca cgccgaccga gatcgcctat ttcctcggcg acgccgaggc gcggctcttc
    13681 gtctgccggc cggaaagcct ggcgacgctg gcgccggtgg cgcgcgaggc cggggtggcg
    13741 gcgctggaga cgctcggcaa cgatggcagc ggcagcctgc tcgaggcggc cgccctggag
    13801 agcgacgcgt tcgcgacggt gccgcgcgcg gccgacgatc tcgccgccat cctctacacc
    13861 tcgggcacga ccgggcgctc gaagggggcg atgctgaccc agcgaaacct tctctccaac
    13921 gccgccacct tggccgagtt ctggcgcttc accgaccggg acgtcctgat ccacgcgctg
    13981 ccgatcttcc acacgcacgg gctcttcgtc gccatcgaca cggtgatgct gagcggcggc
    14041 acgatgatct ggcgggcccg tttcgatgcc gacgaggtga tcggcctgat gggtcgcgcc
    14101 acgtcgctga tgggggtgcc gacc
//
LOCUS       DAOTFI010000125        14120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5184_length_14120_cov_6.698614, whole genome shotgun sequence.
ACCESSION   DAOTFI010000125 DAOTFI010000000
VERSION     DAOTFI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14120
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5184_length_14120_cov_6.698614"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..607)
                     /locus_tag="P5558_13645"
     CDS             complement(170..607)
                     /locus_tag="P5558_13645"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRY25402.1"
                     /translation="MPKAYASTVIDRPAREVWAVVRDFNGLPGWLPAVTRSAIEDGQP
                     ADRIGCVRSIHLADGAHIRERLLTLSDQDMSFTYNFETTPFEVESYLATLRCTSVTDG
                     DRCFVEWWTHFDCDLAKRDEWLDFFANGVFQGGFDGLKERLRR"
     gene            767..1084
                     /locus_tag="P5558_13650"
     CDS             767..1084
                     /locus_tag="P5558_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25403.1"
                     /translation="MRELGLALAALATLAAGAAKAEEAAPAVPRPCHDYKALVTALDK
                     RYGEAPVSLGMQNNGHALQVFASAESGSWTVLSVAPDGTGCIVAAGRHWQTRKPGTSD
                     PAA"
     gene            1166..2890
                     /locus_tag="P5558_13655"
     CDS             1166..2890
                     /locus_tag="P5558_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY25404.1"
                     /translation="MAGDTAEGQRRAKRGDGKGRRTARGRQVDPRAAGEIRELLGDRP
                     RRPDLLIEHLHLIQDRFGHLGAAHLAALAEEMKLAQVEVYEVATFYAHFDVVKEGETP
                     PPPVTLRVCDSLTCALLGADRLMAELAAAADPSKIRVVPAPCMGACHRAPNVAVGHRR
                     VGHATASSVLAAATDGHGAVELPEAIELSQYRASGGYALFERLRDQPPGDADSGPVAE
                     FIKRLEGSGLRGLGGAGFPTGRKWQIVRAQPGPRHLCVNADEGEPGTFKDGHYLTTDP
                     HRFLEGMLIAGWSIEAEKCWIYLRDEYPEARHLLAREILALEQAGLVAPGHIELRRGA
                     GSYICGEESAMIESLEGKRGLPRHRPPFVAEVGLFGRPTLVNNVETLYWIRDIVERGP
                     EWFAAQGRNGSKGLRSYSVSGRVRDPGVKLAPAGITAAELIDEFCGGMADGHRFRAYL
                     PGGASGGILPASMADIPLDFGKLEHLGCFVGSHAVVVLSDQDDVRAAALNLLRFFREE
                     SCGQCTPCRSGTAKAVALMERETWDQALLAELATAMTDASICGLGQAAGNPIRSVMRF
                     FPEAFQNP"
     gene            complement(2912..3838)
                     /locus_tag="P5558_13660"
     CDS             complement(2912..3838)
                     /locus_tag="P5558_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRY25405.1"
                     /translation="MPTIVISEFMDEAAVAELGRHHPTTYDQTLVDQPQALAALLADA
                     RALVVRNRTQVRGALLEAAPRLEAVGRLGVGLDNIDLDACKARGIEVFPASGANDLSV
                     AEYVITTSLMLLRGAYAANAQMLAGEWPRNALIGRELAGRTMGLVGLGSIARLVKAKA
                     LALGMRVAAFDPYLPNGDPAWDRVRKCALDELLGVADVVSLHVPLTDGTRHLIDDAAL
                     RRMRPDAILVNAARGGVVDEAALGEALRQRRLGGAALDVFATEPLTREAAAAFAGAPN
                     LVLTPHIAGVTEESNVRVSSLIASRILAALAG"
     gene            3918..4835
                     /locus_tag="P5558_13665"
     CDS             3918..4835
                     /locus_tag="P5558_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY25406.1"
                     /translation="MARVLTLGITVLDTVLGLARLPSGGGKHYARARAEVVGGIAANA
                     AIAIVRLGGEALLASRFGDDLVGERLQAELAATGVDTSRVEWLGSVASSISTILVDDK
                     GERLLVNHSDPALFHGPASPFDDLVVDAVMTDTLWPDGAIPALQRARALAVPGVLDFD
                     RVPRRMTGELLAAASHVVFGQQGLEAFAGTTSIETGLRSAARQTDAWLACTAGGDGVY
                     WLDGAALRHLPAFPIEAVDTLGAGDVFHGAFALALGEGRGIEASLRFASAAAALKCTG
                     FGSGAMSPGRAEVERFLVEQSDLPVVEPR"
     gene            4893..5123
                     /locus_tag="P5558_13670"
     CDS             4893..5123
                     /locus_tag="P5558_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRY25407.1"
                     /translation="MATLLVRKLDDNLVDRLKRRAASHGRSVEAEHRAILEEALEPRG
                     MSGREFLEIMSRAPRVELELPEDEEIQPATFD"
     gene            5120..5548
                     /locus_tag="P5558_13675"
     CDS             5120..5548
                     /locus_tag="P5558_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY25408.1"
                     /translation="MSGYVLDTNLVSETARAKPDALALDWLSAQPEGSLFLTATVIAE
                     LASGVERMPHGRRRELVAGWLQDAVLNRFGDRILPFDLEAALEYGALIARARAAGRPA
                     TIADTQIAAVASVHGLGVATRDVADYAGFGVPLVNPWQGA"
     gene            5588..6628
                     /locus_tag="P5558_13680"
     CDS             5588..6628
                     /locus_tag="P5558_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY25409.1"
                     /translation="MRYAIIGSGMMGQEHIENIKLLPDSSVVALADPDEGMRRQAAGR
                     CGPEVRTFADYRELLGAGGIDALVIVTPNYTHRAVLMDALATGLPILTEKPLCTTVED
                     CDAVLAAAEGRAAPVWVAMEYRFMPPVARLIEEVRKGTVGRLRMLSIREHRFPFLKKV
                     GDWNRFARNTGGTMVEKCCHYFDLMRLIVGAEAVRLYASGGQDVNHLDETYGGERPDI
                     VDNGYVVVDFANGVRAMLDLCMFAEVSRDQEEIAAVGDRGKVECGTPSSRLWIGRRDT
                     GELTEEVVPVDETVLAAGQHHGSTYYQHRAFLELVKQGGRPEVSLHDGRMAVMMGAAA
                     ERSMRERVPVTF"
     gene            6647..8137
                     /locus_tag="P5558_13685"
     CDS             6647..8137
                     /locus_tag="P5558_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HRY25410.1"
                     /translation="MVFSSYLFILVFLPIVWLGYCLLARSRVGSFGVTLWLLLASLFF
                     YGHWSWRVVPLILLSIAVNWLVGRLIEGSTGRRARLWLALGIAFDLGLLGYFKYANFL
                     VATVESVSGASFAWQTVVLPIGISFFTFQQIAYLVDIYRQEAVERDPWRYALFVTFFP
                     QLIAGPIVHHREMMPQIGRQPRERRVPDLVLGIVIFAIGLAKKVIIADGIAPISDAVF
                     DLAAMGAVPSTGEAWAGALAFGFQIYFDFSGYSDMAVGLARMFGIALPVNFASPYKAS
                     SIIEFWQRWHITLSRFLRDYLYIPMGGGRVGEWRRYRNIVVTMLLGGIWHGAGWTFVL
                     WGLLHGLLITADHARRRFWCGPDLPLWGARALTFALVTIAWVPFRAADLATTFRLYGA
                     MTGMGPSVEATAWPFYSPAGVWLLLAALLAFAWLLPNTAEIMKPAVAPLTTPGYPAVE
                     PVGRAAGWTMGLGFHPSGGWALAIALLLSLSLIGVGNGGAFIYYQF"
     gene            8169..9104
                     /locus_tag="P5558_13690"
     CDS             8169..9104
                     /locus_tag="P5558_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25411.1"
                     /translation="MPLVRFGLRLLWLAPLVFVLLTGVNALALRFDIAALPELANFHQ
                     QQRKLAAAADGGFDTLFLGDSSLGRMMDTPTWERITGERSLQLALTGGEGFAGDLGML
                     QRALLVHRPKRVVLVHTSDMPTRGISWNGYLLTRPAGAARVAMPPDRLLEALADLLIN
                     GQLLRAIVRGLPYSLTGRDWPFLDDAHVRGRERLLGAGPAIAQAEALAPSAIKAEGRL
                     FLEKIAALCAAEGIDCRFAFGPVWEGICANSADYLAAARAFVEGAGLPVVAGTPLCVP
                     AQDLDDTIDHVVQGRMVFYTKALHDRLEGQATTRN"
     gene            9187..9750
                     /locus_tag="P5558_13695"
     CDS             9187..9750
                     /locus_tag="P5558_13695"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY25412.1"
                     /translation="MQTGLNRLIAGFERVNDVTCWVGRQLAWLLVVLMTAAVLLQVFY
                     RYVLVNPIGWSEELAIYSMIWMTFLVAPIAYRSGANVAIEVVRDLFVGRWQAALQIVL
                     NLLVLVLLIVLLRHSITYVGRGMGSLVPSLGVQAGWFYLSMPVGIAGMLLVAVEILLK
                     AVRHLMDPSQPLLMPTHEAEIAASEYE"
     gene            9751..11094
                     /locus_tag="P5558_13700"
     CDS             9751..11094
                     /locus_tag="P5558_13700"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY25413.1"
                     /translation="MALLPFWIFMALLMVGMPVVFALLLGPGFSLWIDGRPDAFFGMM
                     QQRLFNGMKSFPLMAVPFFILAGELMNRGNITLALVEFSKSLIGHVRGGLAQVNILSS
                     ILFAGLSGSAVADTSALGSMLIPAMEQNGYTRRFAAAITAASSVIGPIIPPSGIMILY
                     AFIMNVSVAGLFAAGIVPGLLIGFGLMLMTWLLAKRYKFPVASEKATWRQRGTALRVA
                     FWPLLTPIILLGGILTGVFTPTEAAAVAAGYALVVTLFVTRTLTVKDLPEVFYTTALS
                     AGTILLLVAAAVSFSSVISLFQTPQVLIGFVLWMADNPYSLLFAVNIMLFIVGMFLDA
                     GPAILILGPILGPAMTSMGIDPMHFAIVMCVNVTVGLATPPMGLVLFVAAGISGERVE
                     RIAWAMMPFLLVEIAVIFLITYVPEVSLTLPRLLGFASWPDCAIDPLNALTCLFM"
     gene            11109..12164
                     /gene="dctP"
                     /locus_tag="P5558_13705"
     CDS             11109..12164
                     /gene="dctP"
                     /locus_tag="P5558_13705"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY25414.1"
                     /translation="MTMAGLRRAALGAVAGLGLLGAAALPAMAQDFTLKVIFLANQDD
                     EDYDGALVFKDYVEAASNGSIAVEIYPGGQLCGNPEECLEALQGGIIEVFITTFGGFG
                     NIVQEAQVMDLPYMFPSDRVAECALGNDTAFFELLRDKTLEQTGNMRLMTIGNTGGWR
                     NFATTAKQIRTPADVAGLRIRTIGSPVQQELVKSMGGAPTSIAWPEVYTSLATGVVEG
                     TKNGITDIVGMKFHEHLKYITLDGHAYMGAMWMMNNDVYQAMSPEQRKIVEDGFDALR
                     WTTIVFPKRRSIEAYQAFKDAGGEIYVPTEEEKAQFVDAAAPIYDWFTGEYGSEWLDV
                     LKGSIADCETAIDATRG"
     gene            complement(12474..12695)
                     /locus_tag="P5558_13710"
     CDS             complement(12474..12695)
                     /locus_tag="P5558_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25415.1"
                     /translation="MPALRLPRLALAMTLVLSAMGLGACESSAGKGAAIGAAGGAAAG
                     LITGENVLRSSAAGAAAGAAGGFVFDMLN"
     gene            complement(12713..13096)
                     /locus_tag="P5558_13715"
     CDS             complement(12713..13096)
                     /locus_tag="P5558_13715"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HRY25416.1"
                     /translation="MRGTALVGGVAGIMMLAASAADSAELAAFDLETSADLAALCGAE
                     PDDPNFSDARQFCFGFVSGVSLFYTTALQADRIKKVVCTDEQLTREQLRANFVAWQAE
                     HPEYADASPLDGFVRSAVNRWPCKE"
     gene            complement(13188..>14120)
                     /locus_tag="P5558_13720"
     CDS             complement(13188..>14120)
                     /locus_tag="P5558_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY25417.1"
                     /translation="VAIPQMIRYGYSRPAAYGVVGGGGTLGILIPPSGPMVLFAVVAD
                     ASIGQLFMAGVVPGLLLATCFALHCMAAQRFAGAKAVRREPRASLMEMLTALRRSFWA
                     LTLPVFVLGGMYLGVFTATEAAGAGALAALLIAMLVYRTLGPLDIWRSAMDAARTSAM
                     LFMILAAAAVFSHVLTKMRIPQEIVTLVTSHDLGQIEFLIAVMGLIFVLGMFLETISI
                     ILITTPIVLPVLAALGIDPIWYGVLLMVNLELALITPPVGMNLFTIKAITKGPIEPII
                     RGVLPYAAILLAGLALLLMFPGIALWLPSTMMGR"
BASE COUNT         2064 a         4834 c         4914 g         2308 t
ORIGIN      
        1 cgaagcgaag caaaagccga tttcgacagc ggaggccaaa atggcacggg ccggagcggc
       61 gtcaccgtcc aagccgacct tctgccccga ggccgtggca accggtgcga cagcgtcggc
      121 gcccgcgccc gatttgccga caaggccacc gagccggaca gcgagcgcct cagcgccgga
      181 gccgttcctt cagcccgtcg aagccgccct ggaagacgcc gttggcgaag aagtcgagcc
      241 actcgtcgcg tttggccaga tcgcagtcga aatgggtcca ccactcgacg aagcagcggt
      301 cgccgtcggt cacgctcgtg cagcggagcg tggcgaggta gctctcgacc tcgaacgggg
      361 tcgtctcgaa attgtaggtg aagctcatgt cctggtccga gagggtgagc agccgctcgc
      421 ggatgtgcgc gccatcggcc agatggatgc tgcgcacgca gccgatccga tcggccggct
      481 ggccgtcctc gatcgccgag cgggtcaccg ccgggagcca gcccggcaag ccgttgaaat
      541 cgcggaccac cgcccagacc tccctggccg gcctgtcgat gaccgtgctc gcataggctt
      601 tcggcatcgg agcctcccca ttcctgcccg tttctcgcat gactgaccta gccccgcccc
      661 gccaccgcgt gcaagcggcg cgatggtaac ccgctgttaa ggacagctgt gctgtcatgc
      721 agatggtcgg tgctccagcc cgacgacagc acgggagttg cccctgatgc gcgaactcgg
      781 tctcgccctc gcggccctcg ccacactcgc ggccggtgcc gcgaaggccg aggaggctgc
      841 gccagcggtc ccacggccat gccacgacta caaggcgctc gtcacggcgc tcgacaagcg
      901 ctacggcgag gcgccggtct cgctcggcat gcagaacaac ggccacgcgc tgcaggtctt
      961 cgcctcggcc gagagcggca gctggaccgt gctctccgtc gccccggacg ggaccggctg
     1021 catcgtcgcc gccggccgcc actggcagac ccgcaagccc gggacgagcg acccggccgc
     1081 ctgaatgcgg gtcgccgtgt cagcggcctt gtgacactgg cgccttcctt gatacgcttc
     1141 ctggacaagc ggggagcggg cgaacatggc gggcgatacg gccgaagggc agcggcgggc
     1201 gaagcgcggc gacggcaagg gccggcgcac cgcgcgcggc cgtcaggtcg acccccgggc
     1261 ggccggggag atccgcgagc tgctcggcga ccggccgcga cgccctgacc tcctgatcga
     1321 gcacctgcat ctgatccagg accggttcgg ccatctcggc gccgcccatc tggcggcact
     1381 ggccgaggaa atgaagctcg cccaggtcga ggtctacgag gtcgcgacct tctatgcgca
     1441 tttcgacgtg gtgaaggagg gcgagacccc gcccccgccg gtcacgctgc gcgtctgcga
     1501 cagcctcacc tgcgcgctgc tcggcgccga ccgcctgatg gcggagctcg ccgccgccgc
     1561 cgatccttcg aagatccggg tcgtgcccgc cccctgcatg ggcgcctgtc accgcgcacc
     1621 caatgtcgcc gtcgggcacc ggcgggtcgg ccatgccacg gcgagctcgg tgctggctgc
     1681 cgcgaccgat ggccacggtg ctgtcgagct gcccgaggcg atcgagctct cgcagtatcg
     1741 ggcgagcggc ggctatgcgc tcttcgagcg gctgcgcgat cagccgcccg gcgatgccga
     1801 tagcgggccg gtcgccgagt tcatcaagag actggagggc tcgggcctgc gcgggctcgg
     1861 cggggccggc ttccccaccg gccgcaaatg gcagatcgtc cgggcccagc cggggccgcg
     1921 ccatctctgc gtcaatgccg acgagggcga gcccggcacc ttcaaggacg gccactatct
     1981 caccaccgac ccgcaccgct ttctcgaagg gatgctgatc gccggctggt cgatcgaggc
     2041 cgagaagtgc tggatctacc tgcgtgacga gtacccggaa gcgcgccatc tgctcgcccg
     2101 cgagatcctg gcgctggagc aggccgggct cgtcgccccg ggccatatcg agctgcgccg
     2161 cggcgccggc tcctacatct gcggcgagga atcggcgatg atcgagagcc tcgagggcaa
     2221 gcgcggcctg ccccggcatc gcccgccctt tgtcgccgag gtcggcctct tcggccggcc
     2281 gacgctcgtg aacaatgtcg agaccctcta ctggatccgc gacatcgtcg agcgcggccc
     2341 cgaatggttc gcggcccagg gccggaacgg gtcgaagggc ctgcgctcct actccgtctc
     2401 cggccgggtc cgagaccccg gggtcaagct ggcgccggcc ggcatcaccg ccgccgagct
     2461 gatcgacgaa ttttgcggcg gcatggccga tggccaccgc tttcgggctt atctgccggg
     2521 tggtgcttcg ggcggcatcc tgccggcgag catggcggat attccgctcg atttcggcaa
     2581 gctcgagcat ctgggctgct tcgtcggcag ccacgccgtc gtggtcctct ccgaccagga
     2641 cgacgtcagg gcggcagcgc tcaatctcct gcgcttcttt cgcgaggaat cctgcggcca
     2701 gtgcaccccc tgccgcagcg gcactgccaa ggccgtggcg ctgatggagc gagagacctg
     2761 ggaccaggcg ctgctcgccg agctcgccac cgcgatgacc gacgcctcga tctgcggcct
     2821 cggccaggcc gccggcaacc ccatccgctc ggtgatgcgg ttctttcccg aggcgttcca
     2881 gaacccctag gcgcggccgc gcgccggcgg ctcagcctgc gagcgcggcg aggatgcggc
     2941 tggcgatcag gctcgagacg cggacattgc tctcctccgt caccccggcg atgtgcgggg
     3001 tgaggacgag gttgggtgcc ccggcgaaag cggcagccgc ctcgcgggtc aggggctcgg
     3061 tggcgaagac gtcgagggcg gcgccgccga gccggcgctg cctgagggcc tcgccgagcg
     3121 ctgcctcgtc gaccacgccg ccgcgggccg cgttgacgag gatggcatcg gggcgcatcc
     3181 gccgcagggc agcgtcgtcg atcaggtggc gcgtgccgtc ggtcagcggc acgtgcagcg
     3241 agacgacgtc ggcgacgccc agaagctcgt cgagcgcgca cttgcgcacg cggtcccagg
     3301 ccggatcgcc gttcggcaga taggggtcga aggcggcgac cctcatgccg agcgccaggg
     3361 ccttcgcctt gaccagccgg gcgatcgagc cgaggccgac gaggcccatg gtgcgcccgg
     3421 cgagctcgcg gccgatcagg gcgttacgcg gccattcgcc ggcgagcatt tgggcgttgg
     3481 cggcgtaggc gccgcgcagc agcatgagcg acgtggtgat cacgtactcg gcgacgctga
     3541 ggtcgttggc gcccgaggcc gggaagacct cgatcccgcg cgccttgcag gcgtcgaggt
     3601 cgatattgtc gagcccgacg ccgagccggc ccaccgcctc gagccttggc gccgcctcga
     3661 gcagggcacc cctgacctgg gtgcggttgc ggacgacgag ggctcgggca tcggcgagga
     3721 gggcggccag cgcttgcggc tggtcgacca gggtctgatc gtaggtggtc gggtggtgcc
     3781 ggccgagctc ggccaccgcc gcctcgtcca tgaactcgct gatcacgatc gtcggcatcg
     3841 tcaccctccc ctgtctcgac ctgctcacgc gcgccgtgct atcggccccg ccatagcagg
     3901 caggtggcgg cggtggaatg gcgcgggttc tgaccctggg catcacggtg ctcgatacgg
     3961 tgctcgggct ggcgcggctg ccgagcgggg gcggcaagca ctacgccagg gcgcgggccg
     4021 aggtggtcgg cggcatcgcc gccaatgccg ccatcgccat cgtccggctc ggcggcgagg
     4081 cgctgctcgc cagccgcttc ggcgacgatc tggtgggcga gcggctgcag gccgagctcg
     4141 ccgccaccgg ggtcgatact tcccgggtcg agtggctcgg cagcgtggcg agctccatct
     4201 cgaccatcct ggtcgacgac aagggtgagc ggctgctcgt caaccacagc gatccggccc
     4261 tcttccacgg gcccgcgtca ccgttcgacg acctcgtggt ggacgccgtc atgaccgaca
     4321 ccctctggcc ggacggcgca atccctgccc tgcagcgcgc ccgggcgctg gctgtgcccg
     4381 gcgtgctcga tttcgaccgg gtgccgcggc gcatgacggg cgagctgctc gcagccgcga
     4441 gccatgtcgt cttcggccag caggggctcg aggcctttgc cggcaccacg tcgatcgaga
     4501 cgggcctgcg gtcggcggcc cgccagaccg acgcctggct cgcctgcacg gcgggcggcg
     4561 acggcgtcta ctggctcgat ggcgccgcgc tccggcatct gccggcgttc ccgatcgagg
     4621 cggtcgacac gctgggcgcc ggcgacgtct tccacggcgc cttcgccctg gctctgggcg
     4681 aagggcgagg gatcgaggcg tcattgcgtt tcgccagcgc cgcggcagcg ctcaagtgca
     4741 caggcttcgg cagcggggcg atgagcccgg gccgggcgga ggtggagcgg tttctggtgg
     4801 agcagtccga cctgcctgtg gtcgagcctc gttgagtcct ggcggcacat ttgctatcat
     4861 aacgatagcc aatgcttggg agtggattgg aaatggcgac gctcctcgtt cgcaagctcg
     4921 atgacaatct ggtcgatcgc ctgaaacggc gggcagcttc gcatgggcgg tcggtcgagg
     4981 ccgagcatcg cgccattctg gaggaggcgc tcgagccacg gggcatgagc ggccgggagt
     5041 tcctcgagat catgagccgc gcgccacgag tcgagctgga gctgcccgag gacgaagaga
     5101 tccagccggc gactttcgat tgagcggcta tgtgctcgat accaatctgg tcagcgagac
     5161 ggcacgcgcg aagcccgatg cactagcgct cgactggctt tcggcgcaac ccgagggctc
     5221 tctgttcctg accgcaacgg tcatcgccga gctggcgtcc ggagtcgagc ggatgccgca
     5281 tggacgccgc cgcgagctgg ttgctggttg gctgcaggat gccgtgctca accgattcgg
     5341 cgaccgaatt cttccgttcg atctcgaggc agcgctcgag tatggagcac tcatagcccg
     5401 ggcgcgcgcc gccggccgtc ccgcaaccat cgccgacacg cagatcgccg ccgtcgcctc
     5461 tgtgcatggt ctcggcgtcg ccacccgcga cgtcgcggac tatgccgggt tcggcgtgcc
     5521 gctggtcaat ccctggcaag gcgcgtaaag cccggcgcct gaaccgcctt accgatcagg
     5581 gacgaccatg cgctacgcga tcatcggctc cggcatgatg ggccaggagc atatcgagaa
     5641 catcaagctt ctgcccgaca gcagcgtcgt ggcgctggcc gacccggacg aggggatgcg
     5701 gcggcaggcg gccggccgct gcgggccgga ggtgcggacc ttcgccgact accgggagct
     5761 tttgggcgcc ggcggcatcg atgcgctggt gatcgtcacg ccgaactaca cccatcgggc
     5821 ggtgctcatg gacgcgctcg cgaccggcct gccgatcctg accgagaagc cgctctgcac
     5881 cacggtcgag gattgcgacg ccgtcctggc ggcggccgag ggacgggcgg cgccggtctg
     5941 ggtggcgatg gagtaccgct tcatgccgcc ggtggcgcgg ctgatcgagg aggtgcgcaa
     6001 ggggacggtc ggcaggctgc ggatgctctc gatccgcgag caccgcttcc cgttcctcaa
     6061 gaaggtcggc gactggaacc gcttcgcccg caacaccggc ggcaccatgg tcgagaagtg
     6121 ctgccactat ttcgacctga tgcggctcat tgtcggggcc gaggcggtcc ggctctacgc
     6181 ctcgggcggg caggacgtga accatctgga cgagacttat ggcggcgagc ggccggacat
     6241 cgtcgacaac ggctatgtcg tcgtggattt cgccaacggc gtcagggcga tgctcgatct
     6301 ctgcatgttc gcggaggtgt cgcgggacca ggaggagatc gcggcggtcg gcgaccgggg
     6361 caaggtcgag tgcggcacgc cgtcctccag gctctggatc ggccggcgcg acacgggcga
     6421 gctgaccgag gaggtggtgc cggtcgacga gaccgtgctc gccgccggcc agcatcatgg
     6481 ctcgacctac taccagcatc gggcctttct cgagctggtg aagcagggcg gcaggccgga
     6541 ggtcagcctg cacgacgggc gcatggcggt catgatgggg gccgccgccg agcggtcgat
     6601 gcgggaacgg gtgccggtga ccttctgagc cgcccgcgcc gccgacgtgg tcttcagctc
     6661 ctacctcttc atcctcgttt tcctgccgat cgtctggctg ggctactgcc tgctcgcccg
     6721 ctcgcgggtc gggtcgttcg gggtgacgct ctggctgctg ctcgcctcgc tcttcttcta
     6781 cggccactgg agctggcggg tcgtgccgct gatcctgctc tcgatcgccg tgaactggct
     6841 ggtcggccgc ctgatcgagg gcagcacggg caggcgcgcc cggctctggc tcgccctcgg
     6901 catcgccttc gacctcgggc tgctcggcta tttcaagtat gccaatttcc tggtcgccac
     6961 ggtcgaatcg gtgagcgggg cgagcttcgc ctggcagacc gtggtgctgc cgatcggcat
     7021 ctccttcttc accttccagc agatcgccta cctcgtcgac atctaccggc aggaggcggt
     7081 cgagcgggat ccatggcgct acgcgctctt cgtcaccttc ttcccgcagc tcatcgccgg
     7141 gccgatcgtg catcaccgcg agatgatgcc gcagatcggc cgccagccga gggagcgccg
     7201 ggtgccggac ctggtgctcg gcatcgtcat cttcgcgatc ggcctcgcca agaaggtgat
     7261 catcgccgat ggcatcgcac cgatctcgga tgcggtcttc gacctcgccg ccatgggggc
     7321 cgtgccctcg accggcgagg cgtgggccgg ggcgctcgcc ttcggctttc agatctactt
     7381 cgatttctcc ggctattccg acatggccgt tggcctcgcg cggatgttcg ggatagcgct
     7441 cccggtcaat ttcgcctcgc cctacaaggc ctcgtccatc atcgagttct ggcagcgctg
     7501 gcacatcacg ctctcgcgct tcctgcgcga ctatctctac atccccatgg gcggcggccg
     7561 ggtcggcgag tggcggcgct accgcaacat cgtcgtcacc atgctcctgg gcggcatctg
     7621 gcacggggcg ggctggacct tcgtgctctg gggcctcctg cacggcctcc tcatcaccgc
     7681 cgatcatgcc cgccgccggt tctggtgcgg cccggacctg ccgctctggg gggcacgcgc
     7741 gctgaccttc gcgctcgtga ccatcgcctg ggtccccttc cgtgccgccg atctcgccac
     7801 gaccttcagg ctctatggcg ccatgaccgg catgggcccg agcgtcgagg ccaccgcctg
     7861 gccgttctac tcgccggccg gggtctggct cctgctggct gccctcctcg ccttcgcctg
     7921 gctcctgccc aacacggcgg agatcatgaa gccggccgtg gcgcccctga ccacgcccgg
     7981 ctaccctgcc gtcgagccgg tcggccgggc agccggctgg accatggggc tcggcttcca
     8041 tccgagcggt ggctgggcgc tggccatcgc gctgcttctg tccctctccc tcatcggcgt
     8101 cggcaacggt ggtgcgttca tctactacca gttctgatct cgccagcgcc gggccggcaa
     8161 gggagagggt gcccctcgtc cgcttcgggc tccggctgct ctggctggcg ccgctcgtct
     8221 tcgtgctcct gacgggcgtg aatgccctgg cgctgcgctt cgacatcgcc gccctgccgg
     8281 agctcgcgaa ctttcatcag cagcagcgca agctcgccgc cgccgccgac gggggcttcg
     8341 acacgctctt tttgggcgac agctcgctcg ggcggatgat ggacacgccc acctgggaga
     8401 ggatcacggg cgagcgcagc ctgcaattgg cgctgaccgg cggcgagggc ttcgccggcg
     8461 atctcggcat gctgcagcga gcgctcctgg tgcaccggcc gaagcgggtc gtcctggtcc
     8521 atacgtccga catgccgacc cgcggcattt cctggaacgg ctacctcctg acccggccgg
     8581 caggtgcggc acgggtcgcc atgccgccgg atcggctgct cgaggcattg gctgatttgc
     8641 tcatcaatgg gcagttgctg cgggcgatcg tgcgcggcct gccctacagc ctcaccggac
     8701 gcgactggcc gttcctcgac gacgcccatg tccgcgggcg cgagcggctg ctcggggccg
     8761 ggccggccat cgcccaggcc gaggccctgg caccgtcggc catcaaggcg gaagggcggt
     8821 tgttcctgga gaagatcgcg gcgctgtgcg ccgccgaggg aatcgactgc cgcttcgcct
     8881 tcgggcctgt ctgggagggc atctgcgcca attcggccga ctatctcgcc gcggcccgcg
     8941 cgttcgtcga aggggcgggc ctgccggtcg tggccggcac ccccctctgc gtgccggccc
     9001 aagacctcga cgataccatc gaccatgtcg tccaggggcg catggtcttc tacacgaagg
     9061 cgctccacga ccgcctggag ggacaggcca ccacgaggaa ctgaatggcg agacgaggcg
     9121 ctggcgctgt tgcctgcccg cgccagttcc gtcatgctcc gcgcacaaga ctttcaggga
     9181 ggcccgatgc agacgggtct gaaccggctg atcgccggct tcgagcgggt caacgatgtc
     9241 acatgctggg tcggcaggca gctcgcctgg ctgctcgttg tgctcatgac ggccgccgtc
     9301 ctgctccagg tcttctatcg ctacgtgctg gtcaacccga tcggctggag cgaggagctc
     9361 gccatctact cgatgatctg gatgaccttc ctggtcgccc cgatcgccta tcgctccggc
     9421 gccaatgtgg cgatcgaggt ggttcgcgac ctcttcgtcg gccgctggca ggcggccttg
     9481 cagatcgtgc tcaacctcct ggtgctggtg ctgctgatcg ttctcctgcg ccattcgatc
     9541 acctatgtcg gccgcggcat gggctcgctg gtgccgagcc tgggcgtgca ggccggctgg
     9601 ttctacctct cgatgccggt cggcatcgcc ggcatgctgc tggtcgccgt cgagatcctg
     9661 ctgaaggccg tgcgccacct catggatccg agccagccgt tgctgatgcc gacgcacgag
     9721 gccgagatcg cggcgagcga gtacgagtag atggccctcc tgcccttctg gatcttcatg
     9781 gccctcctga tggtcggcat gccggtcgtc ttcgcgctgc tgctcggccc gggcttcagc
     9841 ctgtggatcg acggccggcc ggatgccttc ttcgggatga tgcagcagcg gctcttcaac
     9901 ggcatgaaga gctttcccct gatggccgtg ccgttcttca tcctggccgg cgagctgatg
     9961 aaccgcggca acatcacgct ggcgctggtc gagttctcga aatcgctgat cgggcatgtc
    10021 cggggcgggc tcgcccaggt caacatcctc tcctcgatcc tcttcgccgg gctctcgggc
    10081 tcggccgtgg ccgacacctc ggccctgggc tcgatgctca ttccggccat ggagcagaac
    10141 ggctacacgc ggcggttcgc ggcggcgatc accgcggcct cctcggtgat cggcccgatc
    10201 atcccgcctt ccgggatcat gatcctctac gccttcatca tgaacgtctc ggtggccggc
    10261 ctcttcgccg ccggcatcgt gcccggcctg ctcatcggct tcggcctgat gctgatgacc
    10321 tggctgctcg ccaagcggta caagttcccg gtggcgagcg agaaggccac ctggcgccag
    10381 cggggcacgg cgctgcgcgt cgccttctgg ccgcttttga cccccatcat cctgctcggc
    10441 ggcatcctca ccggcgtctt cacgccgacc gaggccgcgg ccgtggccgc cggctacgcg
    10501 ctggtcgtca cgctcttcgt cacccgcacg ctgaccgtga aggacctgcc cgaggtcttc
    10561 tacacgacgg ccctctcggc cggcacgatc ctccttctgg tggcggccgc ggtcagcttc
    10621 tccagcgtca tcagcctctt ccagacgccg caggtgctga tcggcttcgt gctgtggatg
    10681 gccgacaacc cctattcgct gctgttcgcc gtcaacatca tgctcttcat cgtcggcatg
    10741 ttcctcgatg ccgggccggc gatcctgatc ctcgggccga tcctcggccc cgccatgacg
    10801 tcgatgggca tcgatccgat gcacttcgcc atcgtcatgt gcgtcaacgt cacggtcggc
    10861 ctcgcgacgc cgcccatggg cctcgtgctc ttcgtcgccg ccggcatctc gggcgagcgg
    10921 gtcgagcgga tcgcctgggc gatgatgccg ttccttctgg tcgagatcgc ggtgatcttc
    10981 ctgatcacct acgtgcccga ggtgtcgctg accctgcccc ggctgctcgg ctttgcgtcg
    11041 tggccggact gcgctatcga tccgttgaac gcgttgacgt gtctcttcat gtgacaaggg
    11101 aggtcgagat gacgatggct ggattgcgac gggcggcgct cggcgccgtc gccgggctgg
    11161 gcctgctggg tgcagcagcg ctaccggcga tggcgcagga cttcaccctc aaggtgatct
    11221 tcctcgccaa ccaggacgac gaggactacg acggtgccct ggtcttcaag gactatgtcg
    11281 aggccgcctc gaacggctcg atcgcggtcg agatctatcc gggcggccag ctctgcggca
    11341 atccggagga gtgcctcgag gccctgcagg gcggcatcat cgaggtcttc atcaccacct
    11401 tcggcggctt cggcaacatc gtccaggaag cgcaggtgat ggacctgccc tacatgttcc
    11461 cgtcggaccg cgtcgccgag tgcgcgctcg gcaacgacac cgccttcttc gagctgctgc
    11521 gtgacaagac gctggagcag accggcaaca tgcggctgat gaccatcggc aacaccggcg
    11581 gctggcgcaa cttcgcgacg acggcaaagc agatcaggac gccggcggat gtcgccggcc
    11641 tgcggatccg caccatcggc tcgccggtgc agcaggagct ggtgaagtcg atgggtggcg
    11701 cccccacctc gatcgcgtgg cccgaggtct acacctcgct ggcgacgggc gtggtcgagg
    11761 gcacgaagaa cggcatcacc gacatcgtcg gcatgaagtt ccacgagcac ctgaaataca
    11821 tcaccctcga cgggcacgcc tacatgggtg cgatgtggat gatgaacaac gacgtctacc
    11881 aggcgatgtc gccggagcag cgcaagatcg tcgaggacgg cttcgacgcg ctgcgctgga
    11941 cgaccatcgt cttccccaag cggcgctcga tcgaggccta ccaggcgttc aaggacgccg
    12001 gtggcgagat ctacgtgccg accgaggagg agaaagcgca gttcgtcgac gcggcagcgc
    12061 cgatctacga ctggttcacc ggcgagtacg gcagcgagtg gctggacgtg ctcaagggct
    12121 cgatcgccga ctgcgagacc gcgatcgacg cgacgcgcgg ctgagcttca ccacgttcga
    12181 acgaccgggg cgcgtgccgc agggcgcgcg cccctcgagt cgctctggcg ccgggctcag
    12241 tgcacccact gcagggtgat gagcaggacg acggcggcgg tcagggcgta gaggccgagg
    12301 gtcgggaaga agtcggacat cgggggcact cgtcaggggt tgcttgctgc agtgcaatat
    12361 cggccgatgg ccggcctccc gcaacgcgcg ggactgcatg gcagccatgc cgacgcaccc
    12421 tgtgggcgcg ccggcacggc aaccccgatc ggctcgcacc cggcggggcg agctcagttc
    12481 agcatgtcga agacgaagcc gccggccgcg ccggcggcag cacccgcggc gctgctgcgg
    12541 agcacgttct cgccggtgat caggccggcg gcggcgccgc ccgcggcacc gatggcggca
    12601 cccttgccag cactgctctc gcaggcgccg agccccatgg cggacagcac cagcgtcatg
    12661 gccagagcca ggcgtggcag gcgcaaagca ggcattccta gttccttcat ggtcattcct
    12721 tgcagggcca acggttgacg gccgaccgga cgaagccgtc gagcgggctc gcatcggcgt
    12781 attccggatg ctcggcctgc caggcgacga agttggcccg aagctgctcg cgggtgagct
    12841 gctcgtccgt gcagacgacc ttcttgatcc ggtcggcctg cagcgcggtc gtgtagaaga
    12901 ggctgacgcc ggagacgaag ccgaagcaga actggcgcgc gtcgctgaaa ttgggatcgt
    12961 cgggctcggc accgcagagt gccgccagat cggccgaggt ctcgagatcg aacgccgcaa
    13021 gctcggcgct gtccgcggct gacgcggcga gcatcatgat gccggcgacg ccacccacga
    13081 gggctgtgcc tcgcatgaat gtcctccttc atgggtttgc cgctgcacaa ctcacggcgt
    13141 ggcgacgact ataatgccgc ttgcgggacg ggcaatgggc gacgcgatca gcgccccatc
    13201 atcgttgaag gcagccagag ggcaatgccg gggaacatca gcagcagcgc caggccggcg
    13261 agcaggatcg ccgcataggg cagcacaccc cggatgatcg gctcgatcgg gcctttggtg
    13321 atggccttga tggtgaagag gttcatgccg accggcgggg tgatcagggc gagctcgaga
    13381 ttgaccatca ggagcacgcc gtaccagatc gggtcgatgc cgagcgcggc cagcaccggc
    13441 aggacgatcg gggtcgtgat caggatgatc gagatggtct cgaggaacat gccgaggacg
    13501 aagatcaggc ccatgacggc gatcaggaac tcgatctggc cgaggtcgtg gctggtgacc
    13561 agggtgacga tctcctgcgg gatgcgcatc ttggtcagca cgtggctgaa gaccgcggcg
    13621 gcggcgagga tcatgaagag catggcggag gtgcgcgcgg cgtccatggc ggagcgccag
    13681 atgtcgagcg ggccgagcgt gcggtagacg agcatggcga tgaggagcgc ggcgagcgcg
    13741 ccggcgccgg cggcctcggt ggcggtgaag acgccgagat acatgccgcc gagcacgaag
    13801 accggcaggg tcagcgccca gaacgagcgg cggagcgcgg tcagcatctc catcaggctg
    13861 gcgcgcggct cgcggcggac ggccttggcg ccggcgaaac gctgggcggc catgcagtga
    13921 agcgcgaagc aagtggcgag gagcaggccc ggcaccacgc ccgccatgaa gagctggccg
    13981 atcgaggcgt cggcgaccac ggcgaagagc accatgggtc ccgacggcgg aatcaggatg
    14041 ccgagcgtgc cgccgccgcc gaccacgcca taggccgccg gccggctgta gccgtagcgg
    14101 atcatctggg ggatggcgac
//
LOCUS       DAOTFI010000126        14099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5193_length_14099_cov_7.243307, whole genome shotgun sequence.
ACCESSION   DAOTFI010000126 DAOTFI010000000
VERSION     DAOTFI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14099
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5193_length_14099_cov_7.243307"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="P5558_13725"
     CDS             <1..637
                     /locus_tag="P5558_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY25418.1"
                     /translation="LHLPAAQMRVAQRARLQDGRRQDGALYPIPDWRPLTGFSIDRAL
                     LFAVMRAESGFDPAAKSSAGALGVMQVMPSTAEAMAKKYDIDYEGAASLNDPATNMAF
                     GQAYLERLRDTWFIGDSVIDIGIAYNAGLKRAEDWAERYGDIDDPLLRLESIPIPETR
                     VYVKKLLGNLWAYRVRLDQPAPELEALAAGGWPQYVGLESSGTERTHARAN"
     gene            621..1163
                     /gene="moaB"
                     /locus_tag="P5558_13730"
     CDS             621..1163
                     /gene="moaB"
                     /locus_tag="P5558_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HRY25419.1"
                     /translation="MPGRIDESLTFRPLGVAVLTVSDTRGLDDDKSGAVLVERLVGAG
                     HSVIDRRILRDDQALIEAQFRAWIADPGVEVVISTGGTGVTGRDVTPEALHAVIEKHV
                     PGFGELFRAQSYEKIGTSSVQSRADAGVANGTYLFVVPGSPGACRDAWDGILAHQLDI
                     RHKPCNFAELLPRLKEHEAG"
     gene            complement(1186..1914)
                     /gene="aqpZ"
                     /locus_tag="P5558_13735"
     CDS             complement(1186..1914)
                     /gene="aqpZ"
                     /locus_tag="P5558_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HRY25420.1"
                     /translation="MQKALGAEFLGTFWLVLGGCGSAVLAAAFPDLGIGFLGVAFAFG
                     LTVLTMAYAVGHISGGHFNPAVSVGLAVAGRFELGKLAPYVIAQVAGGIAAAIVLYLI
                     VSGKADFAGVGGFAANGYGELSPGGYSMASGFLTEIVMTFFFLMIILGASSKRAPAGF
                     APIAIGLGLTLIHLISIPVTNTSVNPARSTSQALFAEGGYLGQLWLFWVAPIIGAALA
                     GLASKWLHLEEDSASAATPAPPSY"
     gene            2040..2570
                     /locus_tag="P5558_13740"
     CDS             2040..2570
                     /locus_tag="P5558_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRY25421.1"
                     /translation="MGLFFTADHHFGHGGARGLFRRPFESTAAMDEAMIARWNEVVGQ
                     GDTVWHLGDFAVRQPAARMAEVLGRLAGTKHLVAGNNDPAATRSLAGWASVHDLIEIE
                     ALGRRLVLCHYPLRSWNLMGKGALQLHGHSHGRLKPMARQVDVGVDCQDFAPVPLARL
                     LAPRKRQLRTDRPATA"
     gene            complement(2542..3855)
                     /locus_tag="P5558_13745"
     CDS             complement(2542..3855)
                     /locus_tag="P5558_13745"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRY25422.1"
                     /translation="MVALAAILTAESALGAGFAIKEQSATALGNAFAGATAGGEDLSY
                     MFFNPAVLGRFDGNQAQLAVSGILTKLNLEEATASTTGGTPITGSNRDGDAAHDAVVP
                     AFYAMASPHEQIRLGIGVNVPFGLGTEYSDGWVGRYHAIKSTFETVNFNPVVAVKATD
                     WLTLAAGAQLQYAEAELTNAIDFGSIGAANLIPGSVPTQQDGQARVKGDGWAYGYNLG
                     VHAEPVKGTRIGLSYRSKIDTTFDGNARFRLDDAGIGATLKGATGAFANTAAETKFEF
                     PPMAGIGVHQAIGEDFAVMAEAQWTGWSTLDELIIKFDNPSQPDNVSEYQWNDSWFFA
                     LGGSWDATRSLKLRVGVAWDQSPVRNRYRSPRVPDSDRYWLATGLGWQIRDNLSLDLG
                     YTHIFFEDADVDQSGTDPGNAARGNLEASYENHIDIVTLSLVYRF"
     gene            complement(4023..5147)
                     /gene="aroB"
                     /locus_tag="P5558_13750"
     CDS             complement(4023..5147)
                     /gene="aroB"
                     /locus_tag="P5558_13750"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRY25423.1"
                     /translation="MSPERLPEGLEVALGDRAYPIHIGGGLLDRAGEILGPLLPAPRA
                     VLVTDSHVAATAHADRLEGSLRTAGIAVQRIVVPPGEGSKSFERLSWLVERILVGGVD
                     RKTALVALGGGVIGDLAGFAAAITLRGLPFVQVPTSLLAQVDSSVGGKTGINTPQGKN
                     LVGAFHQPLAVLIDVDTLNDLPERELRAGYAEIIKHGAIADAAYFAWLERHAAAMLAG
                     DQALRVEAIRRSVEIKAAVVARDERETSGERALLNFGHTFGHAYEAVAGYGGGLLHGE
                     AVAIGMVKAARLSTRLGHAPAEAAERLEAHLEALGIATSPRRIRNEGFAVDALLAAMG
                     RDKKAEAGTVRFVLWRQVGEAFLTARVDPATLDAVLQDDH"
     gene            complement(5137..5733)
                     /locus_tag="P5558_13755"
     CDS             complement(5137..5733)
                     /locus_tag="P5558_13755"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRY25424.1"
                     /translation="MSTDSQPRFRVVGSDPAPPGRLLRPIVLVGLMGAGKTAVGRRLA
                     AALGVAFADADEAIVEAAGMSIPDIFEVYGETAFRDLERRVVVRLLDEATGVLALGGG
                     AFIDPQTRAAVGERGISIWLKAELETLVARVTRKRAVRPLLMQGEPRTILAGLMERRY
                     PIYAEADFTVLTGDQPLDEVVDRLTELLQREGLIRDVA"
     gene            5948..6088
                     /locus_tag="P5558_13760"
     CDS             5948..6088
                     /locus_tag="P5558_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25425.1"
                     /translation="MSTVRRSLLLTLLVVILGSVIFLATWDHPPEVRQIELVVPDEQL
                     PR"
     gene            6099..7073
                     /gene="xerD"
                     /locus_tag="P5558_13765"
     CDS             6099..7073
                     /gene="xerD"
                     /locus_tag="P5558_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRY25426.1"
                     /translation="MLEPFLEMLAVERGASRHTLTAYRRDLDDAAAFLRRRGIVLEQA
                     TAEDLRAFLARLDGQGLKSSTIARKLSALRQYFRFLYLERARPDEPTQHLDRPKLARS
                     LPRYLSIAEVTALLDTAARRPGDEGVRLTALLELLYATGLRVSELVGLPLAALAHDRQ
                     SLRVRGKGDKERMVPIGGAARRALERWLALRRGQDLAASQRQWLFPSRSRQGHMTRQR
                     LAQLLAEVAVEAGIDPGRLSPHVLRHAFASHLLAGGADLRTVQAMLGHADIATTEIYT
                     HLQPERLQQVVATHHPLAVSGRNVQTGALQGSGGAAAAPSKRRRGGPP"
     gene            7070..8053
                     /locus_tag="P5558_13770"
     CDS             7070..8053
                     /locus_tag="P5558_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRY25427.1"
                     /translation="MSFTIIDQAKPRLNRSELAVPGSSPQLFEKAAQSAVDVIFLDLE
                     DAVAPDQKEQARKNVIQALNDIDWGTKTVSVRINGLDTHYMYKDVVDVIEQGGERLDL
                     FMIPKVGTAADVYAVDMLTSQVEAAVGRRKRLGFELIIETALGMANVDAIAAASKRNE
                     SLHFGVADYAASTRARTTMIGGPNPGYGVLTGGADDTDREYHWGDMWHYAVARMVVAA
                     RAWGLRPIDGPFGDFSDAEGYKAQASRAAVLGCEGKWAIHPKQIDLANEMMGPSAADV
                     EKARRILAAMEQATKEGKGAVSLDGRLIDIASIKQAETLVKKAEQIAAAAA"
     gene            8088..9041
                     /locus_tag="P5558_13775"
     CDS             8088..9041
                     /locus_tag="P5558_13775"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRY25428.1"
                     /translation="MSSLLDFEKPIAELEGKVKELRHLANGSDLDLEEEIARLEGRAS
                     KLLSTTYGKLTPWQKTQVARHADRPHFSDYVAALVDEFTPLAGDRCFGDDAAIIGGLG
                     RLKGRPVLVIGHEKGRETEDRVRHNFGMARPEGYRKAVRLMRLAERFGLPVVTLVDTP
                     GAHPGIRAEERGQAEAIARSIETCLGLEVPIVSVIVGEGGSGGAIALATADRVLMLEH
                     SIYSVISPEGCAAILWRNSDQAEAAAEALKLTAQDLLGLGLVDEIIAEPLGGAHRGRG
                     TTIETVGHAISRHLAALDGTPPHEIRRRRREKYLHMGRELE"
     gene            complement(9048..9782)
                     /locus_tag="P5558_13780"
     CDS             complement(9048..9782)
                     /locus_tag="P5558_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HRY25429.1"
                     /translation="MSEPRSPPPLWRRLLPLVVLLVIAFAVYGSGLHRYASFEALEQH
                     HRYLDELVAARPVASGLGFVLIYAIATAVSIPGAVFLTIAGGFLFGMLWGAALSVTGA
                     SIGAIGVFLVARTSLGDYLRSTAAPWLGRMRKGFNENALSYLLVLRIIPLFPFWAVNI
                     VPALLGVELRAYALATVIGIIPGALVFASVGSGLDTVIDRGGEPDLDLILEPAILGPL
                     VGLAVLALLPVVYKRLKGHRRADESR"
     gene            complement(9779..11191)
                     /locus_tag="P5558_13785"
     CDS             complement(9779..11191)
                     /locus_tag="P5558_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY25430.1"
                     /translation="MALLEPDICIIGAGSAGLSVAAGAARLGAETVLIEAGAMGGDCL
                     NVGCVPSKSLIAAAAHAHAMRSAGPFGIEPVRPVVHMGRVRRHIDEVIAGIAPHDSAL
                     RFEGLGVRVIRAHGRFGDARTVEAGGSTIRARRFVLATGSRPAIPPIPGLDTVPFLTN
                     ETIFANETLPEHLLVLGGGPIGVELAQAYRRLGARVTVLDIGPLLPRDDPELTAVVRE
                     ALLGEGVELVERAAIRRVEAGPVVVVGTEGSEQRIAGSHLLVATGRQPAVDELGLDAA
                     GIAHDTHGITVDRGLRTTNRRVYAIGDVAGGPQFTHVAGYHASLVIKSALFRLPARVS
                     DRAIPHVTYTDPALAQVGLDAATAEQRGIAHEVVRWPFALNDRARTARATAGLIKVVA
                     SPRGRVLGAGIAGAGAGELILPWVLATERRLKLSTMASLVVPYPTLGEVGKSAAGAFF
                     TERLFGERTRKLVAFLRWFG"
     gene            11311..11817
                     /locus_tag="P5558_13790"
     CDS             11311..11817
                     /locus_tag="P5558_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRY25431.1"
                     /translation="MPMTRLELFACLDGLDIAHATVEHPPVFTVEQAQEHTHHLPGGH
                     TKNLFLEDKAGGLWLVTCLDEQPVRVNALARLLGAPRFSFGKPERLMEALGVEPGSVT
                     ALALVNDTARRVQPVLDTKLMAHELVNCHPLANTATTTLRSADLLRFMEALGFAPRLV
                     DLDLTLEA"
     gene            11882..12472
                     /locus_tag="P5558_13795"
     CDS             11882..12472
                     /locus_tag="P5558_13795"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY25432.1"
                     /translation="MLKLGLAIALALIIAASTATAQTSQFEKGIEAAHRGEYALAIEI
                     WRPLADSGDLKAQTYIGLMYDNGWGVPQDYAEAHRWFLRAAEKGYAKAQYHLAFSYHQ
                     GRGVQRSQTEALKWYNLAAAQGNGPAQYNLGRIYAHGLVVRRDLVEAYKWLALAAEDP
                     SSIAPLAARDRDLLVPKLTPEQLEVAEQRVKDWRPT"
     gene            complement(12477..13082)
                     /gene="msrA"
                     /locus_tag="P5558_13800"
     CDS             complement(12477..13082)
                     /gene="msrA"
                     /locus_tag="P5558_13800"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRY25433.1"
                     /translation="MRHVSSAVLGAALLLIATACSQARATEYAILAGGCFWCVEADMD
                     AVPGVLETRSGYIGGKFDNPTYESHSAAGDLEAVEVTFDPAVVTYRELLDIFWRTVDV
                     TDDGGQFCDRGNSYRTAVFVADAEERATAEASKAAAEEALGQEIVTRILDAPTFWPAE
                     DYHQNYYTENPLRYAGYRFACGRNRQVEQVWGDQAYEGLAK"
     gene            13360..>14099
                     /locus_tag="P5558_13805"
     CDS             13360..>14099
                     /locus_tag="P5558_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRY25434.1"
                     /translation="MSKIIGIDLGTTNSCVAVMDGKNARVIENEEGVRTTPSMVAFTE
                     DGEVLVGMPAKRQAVTNPENTLFAVKRLIGRRFDDPVVAKDKGMVPYKIVKADNGDAW
                     VEVRGDKKAPSQISAEVLKKMKETAERHLGAKVEKAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGMDKGKNQTVAVFDLGGGTFDISVLEIADDVFEVKSTNG
                     DTFLGGEDFDVRLIDYMADEFKKEQGIDL"
BASE COUNT         2264 a         4809 c         5032 g         1994 t
ORIGIN      
        1 gctgcatttg ccggcggcgc agatgcgggt ggcgcagcgc gcccgcctgc aggacggccg
       61 gcgccaggac ggcgccctct accccatccc cgactggcgg ccgctgaccg gcttcagcat
      121 cgaccgcgcc ctgctcttcg cggtgatgcg ggcggagagc ggcttcgatc cggcggcgaa
      181 aagcagcgcc ggggccctgg gcgtgatgca ggtcatgccg agcaccgccg aggccatggc
      241 caagaaatac gacatcgact acgagggcgc cgcgagcctg aacgatccgg cgaccaacat
      301 ggccttcggt caggcctatc tcgagcgcct gcgcgacacc tggttcatcg gcgacagcgt
      361 catcgacatc ggcatcgcct acaatgccgg gctgaagcgg gccgaggact gggccgagcg
      421 ctacggcgac atcgacgacc cgctcctgcg cctcgagagc atcccgattc ccgagacccg
      481 ggtctacgtc aagaagctgc tcggcaatct ctgggcctat cgggtgcggc tcgaccagcc
      541 ggcgccggag ctcgaggccc ttgccgcggg cggctggccc caatatgtcg ggctggagtc
      601 gagcggtacg gagcgcacac atgcccgggc gaattgacga gagcctgacg ttccggccct
      661 tgggggtcgc cgtgctgacg gtctcggaca cgcgcgggct cgatgacgac aagtccggcg
      721 cggtcctggt cgagcggctg gtcggcgccg ggcacagcgt catcgaccga cgcatcctgc
      781 gcgacgacca ggccctgatc gaggcgcagt tccgcgcctg gatcgccgat ccgggcgtcg
      841 aggtggtgat cagcaccggc ggcaccggag tgaccggtcg cgatgtcacg cccgaggcgc
      901 tgcatgcggt gatcgagaag cacgtgcccg gcttcggcga gctcttccgc gcccagtcct
      961 acgagaagat cggcacgtcc agcgtgcagt ccagggccga tgccggggtg gcgaacggca
     1021 cctatctctt cgtcgtgccg ggctcgcccg gcgcctgccg ggacgcctgg gacggcatcc
     1081 tggcgcacca gctcgacatc cgccacaagc cctgcaactt cgccgagctc ctgccgcggc
     1141 tcaaggagca cgaggccggc tgagccggcc tcgctcctcg ttcgctcagt aggacggtgg
     1201 ggctggtgtg gccgcggacg cgctgtcctc ttccagatgg agccacttcg aggcgagacc
     1261 ggcgagcgcc gcgccgatga tcggggctac ccagaagagc cagagctgac cgagatagcc
     1321 gccttcggcg aacagcgcct ggctggtgct gcgcgccggg ttgaccgagg tgttggtgac
     1381 cgggatgctg atcaggtgga tcagcgtcag gccgaggccg atggcgatgg gggcgaagcc
     1441 ggcgggcgcg cgcttgctgc tggcgccgag gatgatcatc aggaagaaga aggtcatgac
     1501 gatctcggtg aggaaccccg acgccatcga gtagccgccc ggtgacagct cgccgtagcc
     1561 attggccgcg aagccgccga caccggcgaa gtcggccttg ccggagacga tgaggtagag
     1621 gacgatggcc gcggcgatgc cgcccgccac ctgcgcgatg acgtaggggg cgagcttgcc
     1681 gagctcgaac ctgccggcga cggcgagccc gacggagaca gcgggattga aatggccgcc
     1741 cgagatgtgg ccgaccgcat aggccatggt gagcacggtg aggccgaagg cgaaggccac
     1801 tccgagaaag ccgatgccga gatcgggaaa ggcggcggcg agcacggcgg agccgcagcc
     1861 gccgaggacg agccagaacg tgccgaggaa ctcggcgccg agggccttct gcatacggga
     1921 agctcctgat cggtcggacc ggcggcggcg cgtgaggcgg ccggcggcaa gttgcgggga
     1981 cgtgcgggga ataggcaatg gcaaccctcc cggcaagccc acatcgggta gtgaaagcca
     2041 tgggtctttt cttcacggca gatcaccatt tcggccatgg cggtgcgcgc ggcctcttcc
     2101 gccggccgtt cgaatccacc gccgccatgg acgaggcgat gatcgcccgc tggaacgaag
     2161 tggtcggcca gggcgacacc gtctggcacc tgggcgattt cgccgtgcgc cagccggccg
     2221 cgcgcatggc cgaagtcctc gggcggctcg ccggcaccaa gcacctcgtc gccggcaaca
     2281 acgacccggc ggcgacccgc tcgctcgccg gctgggcctc tgtgcacgac ctgatcgaga
     2341 tcgaggccct gggtcgccgg ctcgtcctct gccactaccc gctccgaagc tggaacctca
     2401 tgggcaaggg tgccttgcaa ctgcacgggc acagccacgg gcggttgaag cccatggccc
     2461 ggcaggtcga tgtcggcgtg gattgccagg acttcgcgcc ggtgccgctg gcccgcctgc
     2521 tcgcgcctcg aaaacgccag ctcagaaccg atagaccagc gacagcgtga cgatgtcgat
     2581 gtggttctcg tagctggctt cgagattgcc acgcgcggcg ttgccggggt cggtgccgct
     2641 ctggtccacg tcggcatcct cgaagaagat gtgggtgtag ccgagatcga gcgagaggtt
     2701 gtcccggatc tgccagccga ggccggtggc gagccagtag cggtcggaat ccgggacgcg
     2761 cggcgagcgg tagcggttgc ggaccggcga ctggtcccag gcgacgccga cgcgcagctt
     2821 caggctcctg gtcgcgtccc atgagccgcc gagcgcgaag aaccaggaat cgttccactg
     2881 gtactcggac acgttgtccg gctggctcgg gttgtcgaac ttgatgatca gctcgtcgag
     2941 cgtgctccag ccggtccact gcgcctcggc catcaccgcg aaatcctcgc cgatcgcctg
     3001 gtgcaccccg atgccggcca tcggcgggaa ctcgaacttg gtctcggccg cggtattggc
     3061 gaaggcaccc gtggcaccct tcagcgtggc gccgatgccg gcatcgtcga gcctgaagcg
     3121 ggcgttgccg tcgaaggtcg tgtcgatctt cgagcggtag gagaggccga tccgcgtccc
     3181 cttcaccggc tcggcatgca cgccgagatt gtagccgtag gcccagccgt cgcccttgac
     3241 ccgcgcctgg ccgtcctgct gggtcggcac cgagcccggg atgaggttgg cggcgccgat
     3301 ggagccgaaa tcgatggcgt tggtgagctc cgcctcggcg tactggagct gcgccccggc
     3361 ggcgagggtg agccagtcgg tggccttgac ggcgacgacc gggttgaagt tgacggtctc
     3421 gaaggtggac ttgatcgcgt gatagcggcc gacccagccg tcgctgtatt cggtgccgag
     3481 gccgaacggc acgttcacgc cgatgccgag ccggatctgc tcgtgcggtg aggccatggc
     3541 gtagaaggcg gggaccacgg cgtcgtgggc cgcgtcgccg tccctgttgc tgccggtgat
     3601 gggcgtgcca ccggtggtcg aggcggtcgc ctcctcgaga ttcagcttgg tgaggatgcc
     3661 gctgaccgcg agctgcgcct ggttgccgtc gaaccggccg agcacggccg ggttgaagaa
     3721 catgtagctg aggtcctccc caccggcggt ggcgccggcg aaggcattgc cgagggcggt
     3781 cgccgattgc tccttgatgg cgaagccggc gccaagggcc gactccgcgg tcaggatggc
     3841 cgccaacgcg accattgccg aagcaccgtg cgccacgcgc atcccgcccg ccccccgagc
     3901 tgtcttctct tggctgagcc cggaaagaag caaccagaag gcaaacagac ggacaagtga
     3961 ctaccgcgcc ggttcagcgc tcgacgcaac ttggccgcga actgtggcgt tgcggcgacg
     4021 cttcaatgat cgtcctgcag gaccgcgtcg agagtggccg ggtcgaccct ggcggtgagg
     4081 aacgcctcgc cgacttggcg ccagaggacg aagcgcaccg tcccggcctc ggccttcttg
     4141 tcgcgcccca tggcagcgag gagcgcatcg acggcgaagc cttcgttccg gatccggcgc
     4201 ggcgaggtgg cgatgccgag cgcttccaga tgcgcctcga gccgctcggc ggcctcggcg
     4261 ggggcatggc cgagccgggt cgagaggcgg gccgccttga ccatgccgat ggccacggcc
     4321 tcgccgtgca ggaggccgcc gccatagccc gccaccgcct cgtaggcatg gccgaacgta
     4381 tggccgaagt tcaggagcgc ccgctcgccc gacgtctcgc gctcgtccct ggcgaccacg
     4441 gcggccttga tctcgaccga gcggcggatg gcctcgaccc tgagcgcctg gtcgccggcc
     4501 agcatcgccg ccgcgtggcg ctcgagccag gcgaaatagg cggcatcggc gatggccccg
     4561 tgcttgatga tctcggcata gcccgcccgc agctcgcgct ccggcaggtc gttcagggtg
     4621 tcgacatcga tcagcacggc caagggctgg tggaaggcgc cgaccaggtt cttgccctgc
     4681 ggcgtgttga tgccggtctt gccgccgacg ctgctgtcga cctgggcgag caggctggtc
     4741 ggcacctgga cgaagggcag gccgcgcagc gtgatcgcgg cggcgaagcc ggcgaggtcg
     4801 ccgatcacgc cgccgccgag ggcgacgagc gccgtcttgc ggtccacgcc accgacgagg
     4861 atccgctcga ccagccacga aaggcgctcg aagctcttgc tgccctcgcc ggggggcacg
     4921 acgatccgct gcaccgcgat gccggccgtt cgcagcgagc cctcgagccg gtcggcatga
     4981 gcggtggcgg cgacatggct gtcggtgacg agcacggccc ggggcgcggg caggagcggg
     5041 ccgaggatct cgcccgcccg gtcgaggagg ccgccgccga tatggatcgg gtaggcgcgg
     5101 tcgcccagcg ccacctcgag accctcgggc aggcgctcag gcgacatcgc ggatcaggcc
     5161 ctctcgctgc agcagctcgg tcagccggtc caccacctcg tcgagcggct ggtccccggt
     5221 gagcacggtg aaatcggcct cggcgtagat cgggtagcgg cgctccatca gcccggccag
     5281 gatggtgcgt ggctcgccct gcatgagcag cgggcggacg gcgcgcttcc tggtgacccg
     5341 ggcgacgagc gtctcgagct ccgccttcag ccagatggag atgccgcgct cgccgacggc
     5401 ggcgcgcgtc tgcgggtcga tgaaggcgcc accgcccagc gccagcacgc ccgtggcctc
     5461 gtcgagcagg cggaccacca cgcgccgctc gagatcgcgg aaggcggttt cgccatagac
     5521 ctcgaagatg tcgggaatgc tcatcccggc cgcctcgacg atcgcctcgt cggcatcggc
     5581 gaaggcgacg ccgagcgcgg ccgccaggcg acgcccgacg gcggtcttgc cggcacccat
     5641 caggccgacc agcacgatcg gccgcagcag ccggcccggt ggtgccggat cgctgccgac
     5701 gacccgaaaa cgaggttggc tgtccgtgga catggtcctg tcgttgaaac ccggagttgc
     5761 aaaggttata gagacttgct caggaagcca ggctcttgag gtcaggcccc acacaggcag
     5821 gctccatgta gctaggccag gagatcgtcc ggcggcaaga gcgcaatgac gggggtgctg
     5881 tcgccgtctc gatcgcccgg ttgcgctgcc cgccggccag ccaccaccga aaggcacgtg
     5941 cccgctgatg tcgaccgtcc gtcgctccct gcttctcacc ctgctcgtcg tcatcctcgg
     6001 ctcggtcatc ttcctcgcga cctgggacca cccgccggag gtccgccaga tcgagctggt
     6061 cgtgccggac gagcagctgc cgcgctgaga gccggtcggt gctcgagccg ttcctcgaga
     6121 tgctcgcggt cgagcgtggc gccagccggc acacgctcac cgcctaccgg cgcgatctcg
     6181 atgacgctgc ggccttcctg cgtcgccggg gcatcgtgct cgagcaggcc acggccgagg
     6241 atctgcgggc cttcctcgcc cggctcgacg ggcagggcct gaagagctcg acgatcgcgc
     6301 gcaagctctc cgcgctgcgc cagtatttcc gcttcctcta tctcgagcgg gcccgccccg
     6361 acgagccgac ccagcatctc gaccggccga agctggcgcg cagcctgccc cgctatctga
     6421 gcatcgccga ggtgaccgca ctgctcgata cggcggcaag gcgcccgggc gacgagggcg
     6481 tgcggctcac ggccctgctc gagctgctct atgccaccgg gctcagggtc agcgagctcg
     6541 tcggcctgcc gctcgcggcc ctggcccacg accggcagag ccttcgggtg cgcggcaagg
     6601 gcgacaagga gcggatggtg ccgatcggcg gtgccgcccg ccgggcgctc gagcgctggc
     6661 tggcgctgcg gcggggtcag gacctggcag ccagccagcg gcagtggctc tttccgtcgc
     6721 gcagccggca gggccacatg acgcgccagc gtctggcgca gctcctggcc gaggtcgccg
     6781 tcgaggccgg catcgacccc ggccgtctct cgccgcatgt gctccggcac gccttcgcca
     6841 gccacctgct cgccggcggc gccgacctgc gcaccgtcca ggcgatgctc ggccatgccg
     6901 acatcgcgac caccgaaatc tacacccatc tgcagcccga gcggctgcag caggtggtgg
     6961 cgacccatca tccccttgcc gtgagcgggc gaaacgtcca aaccggcgcc ctgcagggca
     7021 gcggcggtgc tgccgccgcg ccatcgaaac gccgacgagg gggaccacca tgagcttcac
     7081 gatcatcgac caggccaagc cgcgactgaa ccggtcagag ctggccgtgc cgggatccag
     7141 cccgcagctc ttcgagaagg cggcgcagag cgccgtcgac gtgatcttcc tcgacctcga
     7201 ggacgcggtg gcgcccgacc agaaggagca ggcgaggaag aacgtcatcc aggcgctgaa
     7261 cgacatcgac tgggggacga agacggtctc ggtccggatc aacggcctcg acacccacta
     7321 catgtacaag gacgtggtcg acgtcatcga gcagggcggc gagcggctcg acctcttcat
     7381 gatcccgaag gtcggcaccg ccgccgacgt ctacgcggtc gacatgctga ccagccaggt
     7441 cgaggcggcg gtcgggcgca ggaagcggct cggcttcgag ctgatcatcg agacggcgct
     7501 cggcatggcc aatgtcgacg ccatcgccgc ggccagcaag cgcaacgaat cgctgcattt
     7561 cggcgtggcc gactatgccg cctcgacccg ggcccggacg acgatgatcg gcggcccgaa
     7621 cccgggctac ggcgtgctga ccggcggtgc ggacgacacc gaccgcgagt atcactgggg
     7681 cgacatgtgg cactacgcgg tcgcccgcat ggtggtcgcc gcgcgcgcct gggggctgcg
     7741 gccgatcgac gggccgttcg gcgacttctc ggatgccgag ggctacaagg cccaagccag
     7801 ccgggcggcg gtcctgggct gcgagggcaa gtgggcgatc caccccaagc agatcgacct
     7861 cgccaacgag atgatgggcc cctcggccgc cgacgtggag aaggccaggc gcatccttgc
     7921 ggcgatggag caggcgacga aggagggcaa gggtgccgtc tcgctcgacg gccggctgat
     7981 cgacatcgcc tcgatcaagc aggccgagac gctggtcaag aaggccgagc agatcgccgc
     8041 cgcggccgcc tgagcgacgg ccggcagcaa ggggctcaaa gccgacgatg agcagtcttc
     8101 tcgacttcga aaagccgatc gccgagctcg agggcaaggt caaggagctg cggcacctcg
     8161 ccaacggcag cgatctcgac ctcgaggagg agatcgcccg gctcgagggc cgcgcctcca
     8221 agctcctgtc caccacctac ggcaagctga cgccatggca gaagacccag gtggcgcggc
     8281 atgccgaccg gccgcatttc tccgactacg tggcggccct ggtcgacgag ttcacgccgc
     8341 tcgccggcga tcgctgcttc ggtgacgatg cggcgatcat cggcgggctc ggccggctga
     8401 agggccggcc ggtcctggtg atcggccacg agaaggggcg cgagacggag gaccgggtgc
     8461 gccacaattt cggcatggcg cggccggaag gctatcgcaa ggcggtccgg ctgatgcggc
     8521 tggccgagcg gttcgggctg ccggtggtga cgctggtcga cacgccgggt gcccatcccg
     8581 gcatccgcgc cgaggagcgt ggccaggcgg aggcgatcgc ccgctcgatc gagacctgcc
     8641 tcggcctcga ggtgccgatc gtctcggtga tcgtcggcga gggcggctcg ggcggggcca
     8701 tcgccctcgc cacggcggat cgcgtcctga tgctggagca cagcatctac tcggtcatct
     8761 cgcccgaggg gtgtgctgcc attctctggc gcaattccga ccaggccgag gcggcggccg
     8821 aggcgctgaa gctcaccgcc caggatctcc tcggcctcgg actggtcgac gagatcatcg
     8881 ccgagccgct cggcggcgcc catcgcggcc gcggcacgac gatcgagacg gtgggccacg
     8941 ccatcagccg ccacctcgcg gcgctcgacg gcaccccgcc gcacgagatc cgtcggcgtc
     9001 gccgcgagaa atacctgcac atgggccgcg agctggaata gccgacccta tctggactcg
     9061 tccgcccggc ggtggccctt caggcgcttg tagacgaccg gcagcagcgc caggacggcg
     9121 aggccgacga gcggcccgag gatcgcgggc tcgaggatca ggtcgagatc cggctcgccg
     9181 ccccggtcga tcaccgtgtc cagcccgctg ccgacgctgg cgaagacgag cgcacccggg
     9241 atgatgccga tgaccgtggc cagcgcatag gcgcgcagct ccacaccgag cagcgccggc
     9301 acgatgttga ccgcccagaa cgggaagagc gggatgatgc ggagcacgag gaggtagctg
     9361 agcgcgttct cgttgaagcc cttgcgcatc cgcccgagcc agggggcggc ggtcgagcgg
     9421 aggtagtcgc cgagcgaggt ccgggcgacg aggaagaccc cgatggcgcc gatcgaggca
     9481 cccgtgacag agagtgcggc accccacaac atgccgaaga gaaagccgcc ggcgatggtc
     9541 aggaagacgg cacccgggat ggagaccgcg gtggcgattg catagatcag gacgaagccg
     9601 aggccgctcg ccacggggcg ggcggcaacc agctcgtcga gataccggtg atgctgctcg
     9661 agcgcctcga agctggcata gcggtggagc cccgagccgt agacggcgaa cgcgatcacg
     9721 agcagcacga cgagcggcag cagccggcgc cagagcggcg gcggggacct gggctcgctc
     9781 atccgaacca cctgaggaag gcgaccagct tcctggtgcg ctcgccgaac aggcgctcgg
     9841 tgaagaaggc gccggcggca ctcttgccca cctcgccgag cgtcggatag ggcacgacga
     9901 ggctcgccat ggtggagagc ttcagccgcc gctccgtggc gagcacccag ggcaggatca
     9961 gctcgccggc cccggcgccg gcgatgcccg cacccagcac ccggccccgt gggctcgcca
    10021 ccaccttgat cagcccggcc gtggcccggg cggtgcgcgc ccggtcgttc agggcgaagg
    10081 gccagcggac gacctcgtgg gcgatgcccc gttgctcggc cgtcgccgcg tcgaggccga
    10141 cctgggcgag ggccggatcg gtgtaggtga catgcgggat cgcccggtcg ctgaccctgg
    10201 ccggcaggcg gaagagcgcg cttttgatca cgaggctggc gtgatagccg gcgacatggg
    10261 tgaattgcgg gccgccggcg acgtcgccga tggcgtagac gcggcggttg gtggtccgca
    10321 gcccccggtc gacggtgatg ccatgggtgt cgtgggcaat gccggcggca tcgaggccga
    10381 gctcgtcgac ggccggctgc cggccggtgg cgaccaggag atgcgagccg gcgatccgct
    10441 gctcgctgcc ctccgtgccg accaccacca cagggccggc ctcgacgcgg cgaatggcgg
    10501 cccgctcgac gagctcgacg ccttcgccga gcagcgcctc gcgcaccacg gcggtgagtt
    10561 ccggatcgtc ccggggcaag agcgggccga tgtcgagcac cgtcacccgg gccccgaggc
    10621 gccggtaggc ctgcgccagc tcgaccccga tcggcccgcc gccgagcacc aggaggtgct
    10681 ccggcagggt ctcgttggcg aagatggtct cgttggtgag aaaggggacg gtgtcgaggc
    10741 ccgggatcgg cgggatggcc ggtcggctgc ccgtggcgag gacaaagcgg cgggcacgga
    10801 tggtcgagcc gccggcctcc actgtccggg cgtcgccgaa ccggccatgc gcccggatga
    10861 cccgcacacc caggccctcg aagcgcaggg cgctgtcgtg cggggcgatg ccggcgatca
    10921 cctcgtcgat atgccggcgc acccggccca tgtgcacgac cggccggacc ggctcgatgc
    10981 cgaagggacc ggcgctgcgc atggcatggg catgcgccgc cgccgcgatc aggctcttcg
    11041 acggcacgca gccgacattg aggcagtcgc cgcccatcgc cccggcctcg atcagcaccg
    11101 tttcggcgcc gagccgggcg gcgccggcgg ctaccgagag cccggcggag ccggcgccga
    11161 tgatgcagat gtcgggctcg agaagtgcca tgtcgtcgct tgcgcccacc atgtcggcca
    11221 agacctaggc agccccgccg gtcacgacaa accacgatgg cgtgacgccc gccgcttgcg
    11281 gccccgccga ggcattgcta ccagcccgcc atgcccatga cccgcctcga gctcttcgcc
    11341 tgcctcgacg gcctcgacat cgcccatgcg acggtcgagc acccgccggt gttcaccgtc
    11401 gagcaggcgc aggagcatac gcatcacctg ccgggcggcc acaccaagaa cctctttctc
    11461 gaggacaagg cgggcgggct ctggctcgtg acctgcctcg acgagcagcc ggtcagggtc
    11521 aatgcgctcg cccgcctgct cggtgcaccg cgcttctcct tcggcaagcc ggaacggctg
    11581 atggaggccc tgggcgtcga gccgggctcg gtgaccgccc tggcgctggt caacgacacc
    11641 gcaaggcggg tgcaaccggt cctcgacacc aagctcatgg cgcacgagct cgtgaactgc
    11701 cacccgctcg ccaacaccgc gacgacgacg ctgcggtccg ccgacctgct gcgcttcatg
    11761 gaggcgctgg gctttgcgcc gcggctcgtc gacctggacc tgacgctcga agcctgaagc
    11821 ccttgcgtcg gcgggcggtt ccgtttttta ttccatgctg cacatccgga ggcatctctt
    11881 catgctgaag ctcggcctgg cgattgccct tgccctgatc atcgccgcct cgaccgcgac
    11941 ggcgcagacc tcgcagttcg agaagggcat cgaggcggcg caccgcggcg agtacgcgct
    12001 cgccatcgag atctggcggc cactcgccga ttccggcgac ctgaaggccc agacctatat
    12061 cgggctgatg tacgacaatg gctggggcgt gccacaggac tacgccgagg cgcatcgctg
    12121 gttcctgcgg gcagcggaga agggttatgc caaggcgcag taccacctcg ccttcagcta
    12181 ccaccagggt cggggcgtgc agcgcagcca gaccgaggcg ctgaagtggt acaacctcgc
    12241 cgccgcccag ggcaacgggc cggcccagta caatctcggc aggatctacg cgcacggcct
    12301 cgtcgtccgt cgcgatctcg tcgaggccta caaatggctg gcgctggcag ccgaggaccc
    12361 ctcgtcgata gcgcccttgg ccgcgcgcga ccgcgacctc ctggtgccga agctgacccc
    12421 cgagcagctc gaggtggccg agcagcgggt gaaggactgg cgcccgacct gaccggctac
    12481 ttcgcgagac cctcgtaggc ctggtcgccc cagacctgct cgacctgccg gttgcgacca
    12541 caggcgaacc ggtagccggc atagcgcagc gggttctctg tgtagtagtt ctggtggtag
    12601 tcctcggccg gccagaaggt cggcgcatcg aggatgcggg tgacgatctc ctggcccagc
    12661 gcctcctcgg cggccgcctt cgaggcctcg gcggtggcgc gttcctcggc atcggcgacg
    12721 aagacggcgg tgcgatagct gttgccgcga tcgcagaact ggccgccgtc gtcggtgacg
    12781 tcgacggtcc gccagaagat gtcgagcagc tcccggtagg tgacgaccgc cggatcgaag
    12841 gtgacctcga ccgcctcgag gtcgccggcg gccgaatggc tctcgtaggt ggggttgtcg
    12901 aacttgccgc cgatatagcc cgagcgggtc tccagcacgc ccggcaccgc gtccatgtcg
    12961 gcttcgacgc accagaagca gccgccggcg agaatggcgt attccgtcgc ccgcgcctgg
    13021 ctgcaggcgg tggcgatcag cagcagtgcc gcgccgagaa cggcgctcga gacgtggcgc
    13081 atgggtttct cccggtgaag ctgtcacgcg gagagatagt cggcgtcgcg acgaagcaaa
    13141 cggcagcggc cgaccgacga cagtgaaaat cgcgcctctg gcgccggttg cagcgtctcg
    13201 ggggggtgct tatatggtcg tttggtcgag cgcccggcga gagtgggcgg cgtcccggcg
    13261 ctggaaccgc caaggccggc cgtcggcgct cccgatccct gaggcttggg cgatcaggaa
    13321 caacaagcat ttttttcgcg tgctgcgcga ggggttcgaa tgagcaagat catcggcatc
    13381 gatctcggta cgactaacag ctgtgtcgcg gtgatggacg gcaagaatgc ccgcgtcatc
    13441 gagaacgagg agggcgtgcg gacgacgccc tcgatggtgg ccttcaccga ggatggcgag
    13501 gttctggtcg gcatgccggc gaagcgccag gcggtgacca atcccgagaa cacgctcttt
    13561 gcggtcaagc ggctgatcgg ccgccggttc gacgatccgg tcgtcgccaa ggacaagggc
    13621 atggtgccct acaagatcgt caaggccgac aacggcgacg cctgggtcga ggtgcgcggc
    13681 gacaagaagg cgccgtcgca gatctcggcc gaggtgctga agaagatgaa ggagacggcc
    13741 gagcgccatc tgggtgcgaa ggtcgagaag gcggtcatca cggtgccggc ctacttcaac
    13801 gacagccagc gccaggcgac caaggatgcc ggcaagatcg ccgggctcga ggtgctgcgc
    13861 atcatcaacg agccgaccgc cgccgccctc gcctatggca tggacaaggg caagaaccag
    13921 acggtcgccg tcttcgatct cggtggtggc acgttcgaca tctcggtcct cgagatcgcc
    13981 gacgacgtgt tcgaggtgaa atccaccaac ggcgacacgt tcctcggcgg cgaggacttc
    14041 gacgtccggc tgatcgacta catggccgac gagttcaaga aggagcaggg catcgacct
//
LOCUS       DAOTFI010000127        14045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5215_length_14045_cov_6.744389, whole genome shotgun sequence.
ACCESSION   DAOTFI010000127 DAOTFI010000000
VERSION     DAOTFI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 14045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14045
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_14045_cov_6.744389"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="P5558_13810"
     CDS             complement(<1..1093)
                     /locus_tag="P5558_13810"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRY25435.1"
                     /translation="MRYLALVTDFDGTLTAHDGLSAEAEAALGRLRVSGRRIVLATGR
                     RLADLLAICPRPQLFDMIVAENGAILYDPANREVTRLAEPPPEALPRELRARGVEPLE
                     IGEIVIATQAPHRRLVQDVIWELGLEAQVIGNRNAVMVLPAGVNKATGLRHALRELGL
                     SRHEVVGIGDAENDHSFLAQCECAVAVADAAPSTRKIAALVTRGGAGDGVIELIDELI
                     DDDLGRMGGGLPQHWVALGQRADGTPVPLLPYGRNMLVAGPSGCGKSTFTAGVIERLI
                     EKDYQACIVDPEGDYGTMREVVALGNHSRTPGTDEILAILENPEISISINLLGTTLAD
                     RPSFFAELVPSLQAMRARTGRPHWIVVDEA"
     gene            1486..1977
                     /locus_tag="P5558_13815"
     CDS             1486..1977
                     /locus_tag="P5558_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRY25436.1"
                     /translation="MKSLKTLAMASVIGLSLGGAALPAAADDIVETAVAAGSFETLVA
                     AVQAAGLVEVLQGEGPFTVFAPTDEAFAALPDGTVEGLLKPENKDQLTAILTYHVVPG
                     MVMAKDAQGAKVELETVEGASLTVDGTGEGVKVNDANVVTADIVADNGVIHVIDAVIL
                     PPG"
     gene            2181..3956
                     /locus_tag="P5558_13820"
     CDS             2181..3956
                     /locus_tag="P5558_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HRY25437.1"
                     /translation="MCGICGEIRFDGGDASAAALQRMMEVMVPRGPDGGGMVLRNGTG
                     FGHRRLKIIDLSEKAAQPMTDGELGLSIAFNGCIYNYPELRAELEGQGYRFFSTGDTE
                     VILKAYHAWGAACVERFKGMFAFAIQERDSGRVVLARDRFGIKPLYLAEARGRLRFAS
                     SLPALLAAGEVDTSIDRVGLAHYMSFHAVVPPPHTILQGVRKLPPATLRTIEPDGTSK
                     DHLYWRLDFARTREDIERPFDEWRDLVLESLRTAVRRRMVADVPVGVLLSGGVDSSVI
                     VGLLAEMGQAGLATFSIGFEEAHGEKGDEFRYSDLIAKEYGTAHHKIFVPSAELIDAL
                     PATIAAMSEPMVSYDNVGFFLLSREVAKHIKVVQSGQGADEVFAGYHWYPPLAASNDV
                     VGDYAKVFFDRDRAKLAAHLNPDYLTPGDAALDFVRDAMALSGADDPVDKALRLDSTV
                     MLVDDPVKRVDNMTMGHGLEARVPFLDHELVELAARIPAEHKLAHGGKGVLKEAARQV
                     IPAAVIDRPKGYFPVPALKYLEGPYLAMVEDALGSPAARERGLFQQTYLDQLFADPKA
                     HITPLRGSELWQVALLEMWLQQHGI"
     gene            3959..5653
                     /gene="ngg"
                     /locus_tag="P5558_13825"
     CDS             3959..5653
                     /gene="ngg"
                     /locus_tag="P5558_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HRY25438.1"
                     /translation="MASEAITGVGNAVVDMGWGRLLFGQTFADAERLVEAIRAEGPGR
                     RDIAAYVSDPHVILAIAPQELFLDPSHTFRLALAGFRPERRKPRGFTIRRLNSLRDAD
                     EVNRIYLAQKMVPVAPEYFWQRRDSRVVTCLVAEDSTTGRVIGTVMGCDHVRAFGDPE
                     HGSSLWCLAVDPQATRPGVGEALVCHLARNFREAGLAQLDLSVLHDNEQAIALYEKLG
                     FERVPVFTLKRKNPINEKLFLGPSPDEGLNPYAKLIVDEARRRGIGVEIVDAEGGFFR
                     LVSGGRSIACRESLSELTSAVAMSLCDDKAVTRRVLAREGIRVPAQIEAGDPALLAAF
                     LEEHGRLVVKPARGEQGRGVAVGLDTMDSVEAAVAEARRLCDRVLVEACAEGEDLRLV
                     VIDYRVVAAAIRRPAHIIGDGQSTVRDLIQRQSRRRSAATGGESRIPLDAETDRALAA
                     AGIGLDEVPAEGREIQVRRTANLHTGGTIHDVTDSVHPALIEAAVTAARTIGIPVVGI
                     DLMVPSPRRADYVFIEANERPGLANHEPQPTAERFVDLLFPLSMPLAARRQRGAVA"
     gene            5650..6810
                     /locus_tag="P5558_13830"
     CDS             5650..6810
                     /locus_tag="P5558_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HRY25439.1"
                     /translation="MSRLAIDIGYCRDVLERLLQIPSPTGYTDTVVRAVSAELTSLGL
                     PLELTRRGAIRSKLPGQSRRPARAIISHLDTLGAQVRGLKENGRLAICSIGNWSARFA
                     EGARCTIFSEKGAYRGTILPLKASGHTFNEEIDTQPVGWDHIEIRVDAFARHRRDLVK
                     LGIDVGDIVAIDPGTEFLDNGFIVSRHLDNKAGVAVMLAALKALREDRAEPPVDIWWL
                     FSIAEEVGVGASSLLAPDIASMVTVDNGTTAPGQNSDEFGVTISMADQTGPFDYHLTK
                     KLVRLCREHDIRHQKDVFRYYRSDSASAIEAGADVRTALITFGVDASHGYERIHMHAL
                     RSLAELITAYVTSPVEIQRDAIELGRLEGFTTQSMAEAEQSLSAEVRLAGQG"
     gene            6885..7139
                     /locus_tag="P5558_13835"
     CDS             6885..7139
                     /locus_tag="P5558_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled coil domain-containing protein"
                     /protein_id="HRY25440.1"
                     /translation="MTEKDAYERKAEAKMREWQADIDKLRAQADQVSADARIEYDRQI
                     AKLVEQQKAARQQYEKLEKASGEAWADMKSGMEKAWADMS"
     gene            7201..8274
                     /locus_tag="P5558_13840"
     CDS             7201..8274
                     /locus_tag="P5558_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding peptidase"
                     /protein_id="HRY25441.1"
                     /translation="MKLFTCADCGQLLFFENALCQRCGRSLGFVAERTSLATLETDGE
                     LLRPLDLPEEAYRWCANAVHGACNWLVPAESEIDYCRACDHNRTVPDLTNQRHLQLWQ
                     DVEAAKHRLIYSLLRFGLPLASKREEPDTGLAFEFLSDEACSFRDDERVMTGHAEGLI
                     TLNLSEADPAAREQARTSMAEPYRTLLGHFRHEVGHYYWERLIRDGGRLDAFRALFGD
                     ERDDYGQALERHHANGPPADWPSRFVSAYASAHPWEDWAETWAHYFHMVDTLETAHAH
                     GVTTRPRAIDDPKLAVEIDFLPYRTADFDRLMNEWPPLTVALNSLNRSMGQPDLYPFV
                     LAEPSETKMRFVHDLIREVTRPS"
     gene            8375..11572
                     /locus_tag="P5558_13845"
     CDS             8375..11572
                     /locus_tag="P5558_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HRY25442.1"
                     /translation="MSPVSTSPRLYAAAPGHLAGESRDEALARIVGLGFDGLWLLGSR
                     PVEAGLLRAIATAGLGPILDLPIARLPAQAALVRRAPELFQPVTPEDHAALDPRRPDT
                     SLMRLADHVDHRALAGTVLALLRPLVEAGAAGCVVRGATLLPPAFLTGLIGELRAIRA
                     DLRIIADTLGAAPAAVLGLAGAGFDHRLNSVAWWDMRQPWFLDQERELAAVAPSLGFP
                     EAPGGARLAPTVPARHRLELARLRLRLAAVASSGLIVPAGFEHGAEAPLEPEGARPGD
                     LEHWRRHAAFDLSDEIRSLNASLAEAGLRGPLVPVSGPSAEAVGLLRLAGGTAEASAS
                     AALLVVNPDSHGAAGLDGGLAIRAGDGRFGAFTEHGGDGRLVPGTKVVVEPAEARLFF
                     ARAARETALPPDPNASARRLKELAADRVAIEGVEPELDGGRFPVKRIVGDRLEVEADI
                     FCDGHDKIAAALVIRHQDDAHFSEVPMTFLVNDRWAASFPLTRIGRWVYTIRGWRDLF
                     AAWRSEVAKKHDAGLDLSLELEEGRRLIEAASREARGEARAAMQACLAAIAAAGERPG
                     ELLHIMLDEDLAAVMARHGPRTNLSSYGRELEVVVSRRAAEFGAWYELMPRSMADDEN
                     RHGTFKDVIQKLPYVRDMGFDVLYFTPIHPIGRTNRKGRNNSLTPAPDDPGSPYAIGS
                     EDGGHKALHKELGSFEDFAALVEAAHAHGLELALDFAIQCSPDHPWIREHPEWFDWRP
                     DGTIRFAENPPKKYEDIVNVHFYRDALPSIWLELRDTVLFWVERGVKIFRVDNPHTKP
                     FPFWEWLIRDVNDAHPDVIFLAEAFTRPKVMHRLAKVGYQQSYSYFTWRNTKAELTEY
                     LTELTQGPARDYMRPNFFANTPDINPVYLQTGGRAGFRVRHVLAATLGSVYGLYCGFE
                     LCEARPVPGKEDYLDSEKYQLRHWDWDRPGHIRDDIRRLNTIRRQNPALQQFTGLGFY
                     NAGNDQILYYGKHTAGFEDFVLVIVNLDPHESQSAAFEVPLWEYGLADWASIGVEDLV
                     SGERFTWTGKMQHVELDPTVRPYAIWRLIPPGRDH"
     gene            11584..>14045
                     /gene="treS"
                     /locus_tag="P5558_13850"
     CDS             11584..>14045
                     /gene="treS"
                     /locus_tag="P5558_13850"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HRY25443.1"
                     /translation="MAPKNKKPVVDRSDPLWYKDAIIYQTHVKSFHDANNDGVGDFAG
                     LTEKLDYIKDLGVSAIWLMPFYPSPLRDDGYDIQDYRSINPAYGTMRDVRKFIDEAHR
                     RDLRVITELVINHTSDQHPWFQRARRAKPGSRYRDYYVWSDTDDKYSDTRIIFLDTEK
                     SNWTWDPVAKAYFWHRFYSHQPDLNFDHPPVMEEIKSLLHYWLKMGIDGLRLDAIPYL
                     IEREGTNCENLPETHVILKRIRAEMDEHFPDRMLLAEANQWPEDTAPYFGDGDECHMA
                     FHFPLMPRMYMAVAQEDRAPITDIMRQTPEIPPNCQWAIFLRNHDELTLEMVTENERD
                     YLWNTYAADRRARINLGIRRRLAPLMDNDRRKIELMNSLLMSMPGTPVLYYGDEIGMG
                     DNIYLGDRDGVRTPMQWSPDRNAGFSRANPQALYLPPVMDPVYGFDAVNVEAQSRSAS
                     SLLQWMRRLITVRRQHKAFGRGTLDFVYPGNRKILAYTRTFEDETIFCVVNLSRNAQP
                     VELQLQAFKGRIPVELLGRTTFPPIGDLPYLLTVPAYGFYWFVLADEVSLPDWHVDLP
                     EPLPEFITLVMSQGWRSLTEPRNRKELEDGVLASFLANQRWFAAKGVGIERVEFGRHF
                     ALPGDSGGPLLAEVIVHLGGGQEQTYLLPLALAWGEDNLAGASPLRPFTLAKIRRGPK
                     VGALYDATAGSDLALALLDGIRHARSVETASGVLRFSRSPAFDAAELAERPEVHRLAV
                     EQSNSSLMLDRAAILKLYRRPLPGPHPEVEIGRFLTDVAGFANTPPLLGSLDFEGEDG
                     ATTLAAAFGFVLNQGDGWAWTL"
BASE COUNT         2197 a         5063 c         4658 g         2127 t
ORIGIN      
        1 gcgcctcgtc cacgacgatc caatgcggcc ggccggtgcg ggcacgcatc gcctgcaggc
       61 tggggacgag ctcggcgaaa aagctcggcc ggtccgccag ggtggtgccc agcaggttga
      121 tgctgatgct gatctcggga ttctcgagga tcgccagtat ctcgtccgtg cccggcgtgc
      181 gggaatggtt gccgagggcg acgacctcgc gcatcgtgcc gtaatcgcct tccggatcga
      241 cgatgcaggc ctgatagtcc ttctcgatca gccgttcgat gacgccggcg gtgaaggtgg
      301 acttgccgca gccggaaggg cccgccacca gcatgttgcg gccgtagggc aggagcggca
      361 cgggcgtgcc gtccgcccgt tgcccgagcg ccacccaatg ctgcggcagc ccgcccccca
      421 tgcgccccag atcgtcgtcg atcagctcgt cgatcagctc gatgaccccg tcgccggcgc
      481 cacccctggt caccagggcg gcgatcttcc tggtcgacgg tgcggcatcg gccacggcga
      541 cggcgcactc gcattgggcg agaaacgaat gatcgttctc ggcgtcgccg atgcccacca
      601 cctcgtgccg cgacaggccg agctcgcgca gcgcgtgccg caggcccgtc gccttgttga
      661 cgccggccgg cagcaccatg accgcgttgc gattgccgat gacctgcgcc tcgagcccga
      721 gctcccagat gacatcctgc acgagccgac ggtgtggtgc ctgcgtcgcg atcacgatct
      781 cgccgatttc gagcggctcg accccgcgcg cccgcagctc ccgcggcagg gcctcgggtg
      841 gtggctcggc gagccgggtc acctcgcgat tggcggggtc gtagaggatg gcgccgttct
      901 cggcgacgat catgtcgaag agctgcggcc gcgggcagat ggcgaggagg tcggcgaggc
      961 gacggccggt cgcgaggacg atccgtctgc cggagacccg caggcgcccg agcgccgcct
     1021 cggcctcggc cgagagcccg tcatgcgccg tcagcgtgcc gtcgaagtcc gtcacgagtg
     1081 ccaggtagcg catcgggaat accttctgtg gcgatcgggc ccgacttact gccgaccgac
     1141 cacggcaacc ctaccatgcc ctgccgagcg aacccagccg gcgaggccag ccggacccgt
     1201 gccgagccgc cctcgtcccc gggtcgacgg atcacggagc ggcgtcgcat gtgcggtatc
     1261 cgcggcgaga tgcgctgcga tgggggcgag gccgcagcct cggcggctcg atctcatcca
     1321 agctcgtaaa tacgcgagac agcttattct ggatcactgt aatattcaaa taaatttgtt
     1381 acaataaccc gttgaagaac gcctgcgtgc cgttagatca tcctctgcaa acgggggatc
     1441 gattgccaga tgcttgatgc atggtgcgta caggagtctc gacgaatgaa gagcttgaag
     1501 acgttggcga tggcgagtgt catcggtctg tcgctcggcg gggcggcgtt gccggccgcg
     1561 gccgacgaca tcgtggaaac ggcggtggcc gccgggtcgt tcgagaccct ggtcgcggcg
     1621 gtgcaggccg ctggcctcgt cgaggtgctg cagggcgagg ggccgttcac cgtgttcgcg
     1681 ccgaccgacg aggcgttcgc cgccttgccg gatggcacgg tggagggcct gctgaagccc
     1741 gagaacaagg accagctcac agcgatcctg acctatcatg tcgtccccgg catggtcatg
     1801 gccaaggacg cgcagggcgc caaggtggag ctggagacgg tcgagggggc cagcctcacg
     1861 gtcgacggca cgggcgaggg cgtcaaggtc aacgacgcca atgtcgtgac cgccgacatc
     1921 gtggcggaca acggtgtgat ccacgtcatc gacgcggtca tcctgccgcc cggctgacga
     1981 cccgccacgc tgttcggtag cgtcgagggt ccccgggccg tgtccgggga ccctcctcga
     2041 ttccggatcg ctcctcacgc ttcgctccgg ccgccgcaac cttgcggctt ttgccaagtt
     2101 gtccggaagc ccctatggtc cgctctttat gccgggccgt cgggccgggg ccttgccgac
     2161 caatcacgga gcgtcgtttc atgtgtggca tctgcggaga gatccgcttc gatgggggcg
     2221 atgcttcggc cgctgccctg cagcggatga tggaggtcat ggtgccgcgc ggccccgacg
     2281 gcggcggcat ggtcctgcgc aacggcaccg gcttcggcca ccggcggctg aagatcatcg
     2341 atctctccga gaaggccgcc cagccgatga ccgacggcga gctcggcctg tcgatcgcct
     2401 tcaacggctg catctacaac tacccggagc tcagggccga gctcgagggc caaggctacc
     2461 gcttcttctc gaccggcgac accgaggtga tcctcaaggc gtaccatgcc tggggtgcag
     2521 cctgcgtcga gcgcttcaag ggcatgttcg ccttcgccat ccaggaacgt gattcgggcc
     2581 gggtggtcct ggcccgcgac cgcttcggca tcaagccgct ctatctggcc gaggcgaggg
     2641 gccggctgcg cttcgcctcg tccttgccgg ccctgctcgc cgccggcgag gtggatacct
     2701 cgatcgaccg ggtcggcctc gcccactaca tgagcttcca tgcggtcgtc ccgccgccgc
     2761 acaccatcct gcagggtgtc aggaagctgc cgcccgcgac gctgcggacc atcgagccgg
     2821 acggcacgtc gaaggatcac ctctactggc ggctcgactt cgcccggacg cgcgaggaca
     2881 tcgagcggcc gttcgacgaa tggcgcgacc tcgtgctcga atcgctgcgg accgccgtca
     2941 ggcggcgcat ggtggccgac gtgccggtcg gcgtcctgct ctcgggcggc gtcgattcca
     3001 gcgtcatcgt cgggctcctc gccgagatgg ggcaggccgg gctcgccacc ttctcgatcg
     3061 gcttcgagga ggcgcatggc gagaagggcg acgagttccg ctactccgac ctgatcgcga
     3121 aggagtacgg caccgcgcat cacaagatct tcgttccttc cgccgagctg atcgacgccc
     3181 tgccggccac catcgccgcc atgtcggagc ccatggtctc ctacgacaac gttggcttct
     3241 tcctcctctc ccgcgaggtg gcgaagcaca tcaaggtggt gcagagcggg cagggggcgg
     3301 acgaggtctt cgccggctat cactggtacc cgccgctggc cgcgtccaac gacgtggtcg
     3361 gcgactacgc gaaggtcttt ttcgaccgcg accgggcgaa gctcgcggcg catctgaacc
     3421 ccgactacct gacgcccggg gacgcggccc tggacttcgt ccgcgacgcc atggcgctct
     3481 ccggcgccga cgacccggtc gacaaggccc ttcggctgga cagcacggtc atgctggtcg
     3541 acgacccggt caagcgggtc gacaacatga ccatgggcca cgggctggaa gcacgggtgc
     3601 cgttcctcga tcacgagctg gtcgagctgg ccgcccgcat cccggccgag cacaagctgg
     3661 cgcacggtgg caagggtgtc ctgaaggagg ccgcaaggca ggtgatcccc gccgcggtga
     3721 tcgaccggcc caagggctat ttcccggtgc cggcgctgaa atacctggag ggcccctatc
     3781 tggcgatggt cgaggacgcg ctcggctcgc cggcggcgcg cgagcgtggc ctcttccagc
     3841 agacctatct ggaccagctc ttcgccgacc ccaaggcgca tatcacgccg cttcgcggct
     3901 ccgagctctg gcaggtggcc ctgctcgaga tgtggctgca gcagcacggg atttgagcat
     3961 ggcgagcgag gcgatcaccg gcgtcggcaa tgccgtcgtc gacatgggct ggggccggct
     4021 gctgttcggc cagaccttcg ccgatgccga gcggctggtc gaggcgatcc gcgccgaggg
     4081 gccgggccgg cgcgacatcg cggcctatgt cagcgacccg cacgtcatcc tggccattgc
     4141 acctcaagag ctcttcctcg acccgtcgca caccttccgc ctcgcgctcg cgggcttccg
     4201 cccggagcgc cggaagcccc ggggcttcac catccgccgg ctcaactcgc tgcgcgatgc
     4261 cgacgaggtc aaccgcatct acctggcgca gaagatggtg ccggtggcgc ccgagtattt
     4321 ctggcagcgg cgcgacagcc gggtggtgac ctgcctcgtc gccgaggaca gcacgaccgg
     4381 gcgcgtcatc gggacggtca tgggttgcga ccatgtccgt gccttcggcg acccggagca
     4441 cggcagctcg ctctggtgcc tcgccgtcga cccgcaggcg acccggcccg gggtcggcga
     4501 ggccctggtg tgtcatctcg cccggaactt cagggaggcc gggttggcgc agctcgacct
     4561 ctcggtgctg cacgacaacg agcaggcgat cgccctctac gagaagctgg ggttcgagcg
     4621 cgtcccggtc ttcaccctca agcgcaagaa cccgatcaac gagaagctgt tcctcgggcc
     4681 ctcgccggac gagggcctga acccctatgc caagctgatc gtcgacgagg ccaggcggcg
     4741 cggcatcggc gtcgagatcg tcgatgccga gggcggcttc tttcgcctcg tctccggcgg
     4801 ccgcagcatc gcctgccgcg agagcctctc cgagctgacc tcggccgtgg cgatgagcct
     4861 gtgcgacgac aaggccgtga cacggcgggt gctggcgcgt gaaggcatcc gggtgccggc
     4921 gcagatcgag gcgggcgatc cggcgctgct cgccgccttc ctcgaggagc atggccggct
     4981 cgtggtcaag ccggcgcggg gcgagcaggg ccggggcgtg gcggtcgggc tcgacaccat
     5041 ggattcggtc gaggcggcgg tcgccgaggc gcgacggctc tgcgatcggg tgctggtcga
     5101 ggcctgcgcc gagggcgagg acctgcggct cgtggtgatc gactatcggg tcgtggccgc
     5161 cgccatccgc cgccccgcgc acatcatcgg cgacggccag tcgacggtgc gcgacctgat
     5221 ccagcgccag agccgccgcc gatcggccgc gaccggcggc gagagccgga tcccgctcga
     5281 tgccgagacc gacagggcgc tcgccgccgc cggcatcggg ctcgacgagg tgccggccga
     5341 ggggcgcgag atccaggtgc ggcgcaccgc caacctgcac accggcggca cgatccacga
     5401 cgtcaccgac agcgtccatc ccgccctcat cgaggcggcg gtgacggcgg cccggaccat
     5461 cggcatcccg gtcgtcggga tcgatctcat ggtgccgtcg ccgcgccgcg ccgactacgt
     5521 cttcatcgag gcgaacgagc ggccgggcct cgccaaccac gagccgcagc cgacggcgga
     5581 gcgcttcgtc gacctcctct tccccctgtc gatgccgctc gccgcacggc gccagcgcgg
     5641 ggccgtggca tgagccggct ggccatcgac atcggctact gccgggacgt gctcgagcgc
     5701 ctgctccaga tcccgagccc gacaggctac accgacaccg tcgtccgcgc cgtctcggcg
     5761 gagctcacca gcctcggcct gccgctcgag ctcacccgtc gcggcgccat ccgcagcaag
     5821 ctgccgggcc agagccgccg cccggcccgg gcgatcatct cgcacctcga cacgctgggc
     5881 gcccaggtcc gcggcctcaa ggagaacggc cggctggcga tctgctcgat cggcaattgg
     5941 tcggcacgct tcgccgaggg tgcccgctgc accatcttca gcgagaaggg cgcctatcgc
     6001 ggcaccatcc tgccgctcaa ggcctcgggc cacaccttca acgaggagat cgacacccag
     6061 ccggtcggct gggaccacat cgagatccgc gtcgacgcct tcgcgcgcca tcggcgggac
     6121 ctcgtgaagc tcggcatcga cgtcggcgac atcgtcgcga tcgatcccgg caccgagttc
     6181 ctcgacaacg gcttcatcgt ctcccgccat ctcgacaaca aggccggcgt cgccgtcatg
     6241 ctggcagcgc tcaaggcgct gcgggaggac cgggccgagc caccggtcga catctggtgg
     6301 ctcttctcga tcgccgagga ggtcggcgtc ggcgcctcgt cgctgctcgc cccggacatc
     6361 gcctcgatgg tcaccgtcga caacggcacc accgcacccg gccagaacag cgacgagttc
     6421 ggcgtcacca tctcgatggc cgaccagacc gggcccttcg actaccacct gaccaagaag
     6481 ctggtgcgcc tctgccgcga gcacgacatt cgccaccaga aggacgtgtt ccgctactat
     6541 cgctccgaca gcgcctcggc gatcgaggcg ggtgccgacg tccgcacggc gctcatcacc
     6601 ttcggcgtcg atgcgagcca cggctacgag cgcatccaca tgcacgcgct gcgctcgctg
     6661 gccgagctga tcaccgccta cgtcacgagc ccggtcgaga tccagcgcga cgcgatcgag
     6721 ctcggccgcc tcgagggctt caccacccag tccatggccg aggccgagca gagcctgtcg
     6781 gcggaggtgc ggctcgccgg ccagggctga gcccgccacc gcgctttggc aggcgacccg
     6841 ccctgggcta tgctcgaggg ctattcgaca acggggagga accgatgacc gagaaagacg
     6901 cttacgagag gaaggccgag gccaagatgc gcgaatggca ggccgatatc gacaagctcc
     6961 gcgcccaggc cgaccaggtc tcggccgacg cgcggatcga gtacgaccgg cagatcgcga
     7021 agctggtgga gcagcagaag gcggcccgcc agcaatacga gaagctggag aaggcatccg
     7081 gcgaggcctg ggccgacatg aagtcgggga tggagaaggc ctgggccgac atgtcgtagg
     7141 cgatgcggga cgcgatgcgg cggttcggct gagcccgggc aacaacgacg ggagtaccgc
     7201 ttgaagctct tcacctgcgc cgattgcggc cagctcctgt tcttcgagaa cgccctctgc
     7261 cagcgctgcg ggcggtcgct cggcttcgtc gcggagcgca cctcgctcgc cacgctcgag
     7321 acggacggcg agctcctccg gccgctggat ttgccggagg aggcctatcg ctggtgcgcc
     7381 aatgccgtgc acggggcctg caactggctg gtcccggcgg agtcggagat cgattattgc
     7441 cgggcctgcg accacaaccg gacggtgccc gacctcacca atcagcgtca cctgcagctc
     7501 tggcaggacg tcgaggcggc caagcaccgg ctgatctaca gtcttctccg cttcggcctc
     7561 ccgctcgcca gcaagcggga ggagccggac accgggctcg ccttcgagtt cctctcggac
     7621 gaggcctgca gctttcgcga tgacgagcgg gtgatgaccg gccatgccga gggcctcatc
     7681 acgctcaacc tcagcgaagc ggaccccgcc gcccgcgagc aggcgcgcac cagcatggcc
     7741 gagccctacc gcaccctgct cgggcatttc cgccacgagg tcgggcacta ctactgggaa
     7801 cggctgatcc gggacggcgg ccggctcgac gcctttcgcg ccctcttcgg tgacgagcgg
     7861 gacgactacg gccaggcgct cgagcggcat catgccaacg ggccgccggc cgactggccg
     7921 agccggttcg tcagcgccta cgcctcggcc catccctggg aggactgggc cgagacctgg
     7981 gcgcactact tccacatggt cgacacgctg gagacggcgc atgcccacgg cgtgaccacg
     8041 cggccacgcg cgatcgacga tccgaagctg gccgtcgaga tcgatttcct cccgtaccgg
     8101 accgccgact tcgaccggct gatgaacgag tggccgcccc tgaccgtggc cctgaacagc
     8161 ctcaaccgca gcatgggcca gcccgacctc tacccgttcg tgctggcgga gccatcggaa
     8221 acgaagatgc gtttcgtcca cgacctgatc cgcgaggtca cgcgaccttc ctgagggatg
     8281 caataccgct tccgcgtggc ccggttcaca agcgcccgtc agttgctatg ctgtgcttca
     8341 gccgaacccc aaaggagagg cagccctgac catcatgtca ccagtcagca cctcgccacg
     8401 gctctatgcc gcggctcccg gccacctcgc cggcgaatcc cgtgacgaag cgctcgcccg
     8461 gatcgtcggc ctcggcttcg acgggctctg gctgctcggc tcgcggccgg tcgaagcggg
     8521 gctgctgcgc gccatcgcca ccgccggcct cggcccgatc ctcgatctcc ccatcgcccg
     8581 cctgccggcc caggccgccc tcgtgcgccg ggcaccggag ctcttccagc cggtgacccc
     8641 ggaggaccac gcggccctcg acccgcggcg gccggacacg agcctcatgc ggctcgccga
     8701 ccatgtcgat catcgggctc tggccgggac cgtcctggcg ctgctgcggc ccttggtcga
     8761 ggcgggtgcg gccggctgcg tggtgcgcgg cgcgaccctg ctgccgccgg ccttcctcac
     8821 cggcctgatc ggcgagctgc gcgccatccg cgccgatctg cggatcattg ccgatacgct
     8881 cggcgcggcg cccgccgccg tcctcgggct cgccggtgcc gggttcgacc accggctgaa
     8941 cagcgtcgcc tggtgggaca tgcggcagcc ctggttcctg gaccaggaac gggagctcgc
     9001 agcggtcgca ccgtcgctcg gcttccccga ggcgccgggc ggcgcccggc tggcgccgac
     9061 cgtgccggcc aggcaccgcc tcgagctggc ccgcctgcgg ctccgtctcg ccgccgtggc
     9121 ctcgtccggg ctgatcgtgc cggcgggctt cgagcatggt gccgaggcgc cgctcgagcc
     9181 ggaaggtgcg cggccgggcg acctcgagca ctggcgccgc cacgccgcgt tcgacctctc
     9241 ggacgagatc cggagcctca atgcgagcct cgccgaggcc ggcctgcggg gaccgctcgt
     9301 gcccgtctcc ggcccgtccg ccgaggcggt gggcctgctc cggctcgccg gcggcacggc
     9361 cgaggcgagc gcttctgccg cccttctggt cgtcaatccc gacagccatg gtgccgccgg
     9421 cctcgatggt ggtctcgcga tccgcgccgg ggacggtcgt ttcggcgcgt tcaccgagca
     9481 tggcggcgat ggccgcctgg tgccgggcac gaaggtggtg gtcgagccgg cggaagcgcg
     9541 gctcttcttc gccagggcgg cgcgcgagac ggccctgccg cccgatccga acgcctcggc
     9601 acggcggctg aaggagctcg ccgccgaccg ggtcgccatc gagggcgtcg agcccgagct
     9661 ggatgggggt cgattccccg tcaagcggat cgtcggcgac cgcctggagg tcgaggccga
     9721 catcttctgc gacggccacg acaagatcgc ggcagcgctc gtgatccgcc accaggacga
     9781 cgcccatttc agcgaagtgc cgatgacctt cctggtcaac gaccgctggg ccgcctcctt
     9841 cccgctcacc cggatcggcc gctgggtcta caccatccgc ggctggcgcg atctcttcgc
     9901 cgcctggcgg agcgaggtgg cgaagaagca cgatgccggg ctcgacctct cgctcgagct
     9961 ggaggagggg aggcgcctga tcgaggccgc ctcgcgcgag gccaggggcg aggccagggc
    10021 cgccatgcag gcctgcctcg ccgccatcgc cgccgccggc gagcggccgg gcgagctctt
    10081 gcacatcatg ctcgacgagg acctcgcggc cgtgatggcg cggcacggcc cgcgcaccaa
    10141 cctctcgtcc tacggccgcg agctcgaggt cgtggtcagc cggcgagcgg ccgagttcgg
    10201 cgcctggtac gagctgatgc cgcgctccat ggccgacgac gagaatcgcc acggcacctt
    10261 caaggacgtc atccagaagc tcccctatgt ccgggacatg ggcttcgacg tgctctactt
    10321 cacgccgatc catccgatcg gccggaccaa ccggaagggc cgcaacaaca gcctgacccc
    10381 ggccccggac gacccgggct cgccctacgc catcgggtcc gaggacggcg gccacaaggc
    10441 gctgcacaag gagctcggct cgttcgagga cttcgctgcc ctggtcgagg cggcccacgc
    10501 ccacggcctc gagctggcgc tcgacttcgc catccagtgc tcgccggacc atccctggat
    10561 cagggagcac ccggaatggt tcgactggcg ccccgacggc acgatcaggt tcgccgagaa
    10621 cccgccgaag aagtacgagg acatcgtcaa cgtccatttc tatcgcgacg cgctgccgtc
    10681 gatctggctc gagctgcgcg acaccgtgct cttctgggtc gagcgggggg tcaagatctt
    10741 ccgcgtcgac aacccgcaca ccaagccctt ccccttctgg gaatggctga tccgcgacgt
    10801 caacgacgcg cacccggacg tgatcttcct cgccgaggcc ttcacccggc ccaaggtcat
    10861 gcaccggctc gccaaggtcg gctaccagca gtcctacagc tacttcacct ggcgcaacac
    10921 caaggccgag ctcaccgaat acctgaccga gctgacccag ggcccggcca gggactacat
    10981 gcggcccaac ttcttcgcca acacgccgga catcaacccg gtctacctgc agaccggcgg
    11041 ccgcgccggc tttcgcgtgc ggcacgtgct ggcggcgacg ctgggctcgg tctacggcct
    11101 ctattgcggc ttcgagctct gcgaggcccg gccggtgccg ggcaaggagg actatctcga
    11161 cagcgagaag taccagctcc gccactggga ctgggaccgc ccgggccaca tccgcgacga
    11221 catcaggcgg ctgaacacga tccgccgcca gaacccggcc ctgcagcagt tcacgggcct
    11281 cggcttctac aacgccggca acgaccagat cctctactac ggcaagcaca cggcgggctt
    11341 cgaggacttc gtcctggtca tcgtcaacct cgacccgcac gagagccagt ccgccgcctt
    11401 cgaggtgccg ctctgggaat acgggctcgc cgactgggcg agcatcggcg tcgaggacct
    11461 cgtcagcggc gagcgcttca cctggaccgg caagatgcag catgtcgagc tcgaccccac
    11521 cgtccgtccc tatgccatct ggcggctgat cccgcccggt cgcgaccact gagaacaggc
    11581 aacatggcac caaaaaacaa gaagcccgtc gtcgatcgct cggatccgct ctggtacaag
    11641 gacgcgatca tctaccagac gcacgtcaag tcgttccacg atgccaacaa tgacggcgtc
    11701 ggcgacttcg ccgggctcac cgagaagctc gactacatca aggacctcgg cgtctcggcg
    11761 atctggctga tgccgttcta cccgtcgccg ctgcgggacg acggctacga catccaggac
    11821 tatcgcagca tcaacccggc ctacggcacg atgcgcgacg tccggaaatt catcgacgag
    11881 gcgcaccggc gcgatctgcg ggtcatcacc gagctggtca tcaaccacac ctccgaccag
    11941 cacccgtggt tccagcgcgc ccggcgggcg aagcccggct cgcgctaccg cgactactat
    12001 gtctggtccg acaccgacga caaatactcg gacacccgga tcatctttct cgacacggag
    12061 aagtcgaact ggacctggga tccggtggcc aaggcctatt tctggcaccg cttctacagc
    12121 caccagcccg acctcaactt cgaccacccg ccggtcatgg aggagatcaa gagcctcctg
    12181 cactactggc tgaagatggg gatcgacggg ctgcggctcg acgccatccc gtatctgatc
    12241 gagcgcgaag gcacaaattg cgagaacctg ccggagacgc acgtcatcct caagcgcatt
    12301 cgcgccgaga tggatgagca cttccccgac cgcatgctgc tcgccgaggc caaccagtgg
    12361 ccggaggaca ccgcccccta tttcggcgac ggcgacgaat gccacatggc gttccacttc
    12421 cccctcatgc cgcgcatgta catggcggta gcccaggagg accgcgcccc gatcaccgac
    12481 atcatgcgcc agacaccgga gatcccgccc aactgccagt gggcgatctt ccttcggaac
    12541 cacgacgagc tgacgctcga gatggtgacc gagaacgagc gcgactacct gtggaacacc
    12601 tacgccgccg accgccgggc gcgcatcaat ctcggcatcc gccgccgcct ggcgccgctg
    12661 atggacaacg accggcgcaa gatcgagctg atgaacagcc tcctgatgtc gatgccgggc
    12721 acgccggtgc tctactacgg cgacgagatc ggcatgggcg acaacatcta tctcggcgac
    12781 cgcgacgggg tcaggacgcc gatgcagtgg tcgccggaca ggaatgccgg cttctccaga
    12841 gccaaccctc aggccctcta tctgccgccg gtcatggatc cggtctacgg cttcgacgcg
    12901 gtcaacgtgg aggcccagag ccgcagcgcc tcgtcgctgc tgcaatggat gcgccggctg
    12961 atcaccgtgc gccgccagca caaggccttc ggtcgcggca cgctcgactt cgtctacccg
    13021 ggcaaccgca agatcctggc ctacacccgc acgttcgagg acgagaccat cttctgcgtg
    13081 gtcaatctct cgcgcaatgc ccagccggtc gagctgcagc tccaggcgtt caagggccgg
    13141 attccggtcg agctcctcgg ccgcaccacc tttccgccga tcggcgatct gccctatctg
    13201 ctgaccgtgc cggcctacgg cttctactgg ttcgtcctcg ccgacgaggt gagcctgccc
    13261 gactggcatg tcgacctgcc cgagcccctg ccggagttca tcaccctggt catgtcccag
    13321 ggctggcggt cgctgaccga gcctcgcaac cgcaaggagc tggaggacgg ggtgctcgcc
    13381 agcttcctcg ccaaccagcg ctggttcgcg gcgaaggggg tcggcatcga gcgcgtcgag
    13441 ttcggccgcc acttcgcgct gccgggcgat tcgggcgggc cgctcctcgc cgaggtcatc
    13501 gtgcatctcg ggggcggcca ggagcagacc tatctcctgc ccctggccct cgcctggggc
    13561 gaggacaacc tcgccggcgc ctcgccgctg cggcccttca ccctcgccaa gatccgccgc
    13621 ggccccaagg tcggtgccct ctacgacgcc accgccggca gcgacctggc gctggcgctg
    13681 ctcgacggca tccgccacgc ccgatcggtc gagaccgcct cgggcgtgct gcgcttcagc
    13741 cggagcccgg ccttcgacgc cgccgagctg gccgaaagac cggaggtcca ccggctcgcg
    13801 gtcgagcaga gcaactcgtc cctgatgctc gaccgcgcgg cgatcctcaa gctctaccgg
    13861 cggcccttgc ccggcccgca cccggaggtc gagatcggcc gctttctcac cgatgtcgcg
    13921 ggcttcgcca acacgccgcc cctgctcggc agcctcgatt tcgagggcga ggacggtgcg
    13981 accacgctgg ccgccgcctt cggctttgtc ctcaaccagg gtgacggctg ggcctggacg
    14041 ctcga
//
LOCUS       DAOTFI010000128        13881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5287_length_13881_cov_6.629973, whole genome shotgun sequence.
ACCESSION   DAOTFI010000128 DAOTFI010000000
VERSION     DAOTFI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5287_length_13881_cov_6.629973"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..856
                     /gene="ugpQ"
                     /locus_tag="P5558_13855"
     CDS             86..856
                     /gene="ugpQ"
                     /locus_tag="P5558_13855"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: protein motif:HMM:NF006989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRY25444.1"
                     /translation="MRTLDVRLAPVIAHRGASADTPENTLAALRAAAAAGARWVEFDV
                     KLSRDGYCILMHDDLLNRTTNGRGEVAHYDLDELRRLDAGGWFGRRFRGEPVPTLQEA
                     LALCVELDLDINLEIKPCPGRQHETAIRVVDELRRHWPAGRSWPLISSFAIASLEEVR
                     KLGPDMPHGLLIGRPTRRALGMLDRLECSTLHCDAKHASAELVRHLTRSGRPLLCYTV
                     NDASRALRLLEMGVRAVFTDRPQLIAEAIAARSAKQGQ"
     gene            complement(850..1593)
                     /gene="lexA"
                     /locus_tag="P5558_13860"
     CDS             complement(850..1593)
                     /gene="lexA"
                     /locus_tag="P5558_13860"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRY25445.1"
                     /translation="MLTQRQLQLLTYIHSYVVEHQVPPSFEEMRNALQLKSKSGVHRL
                     ISGLEERGYIRRLAYRARALEIVRGPHGGPLADFSTEGPAAAAGSGANAVGRARPSSN
                     IVRGRFGAGPRATPVSESGSRSLPLVGRIAAGAPIEALLDQSTAIDVPGLLLGEGEHY
                     VLQVSGDSMVDAGIVDGDYAVIQRCETSENGSIVVALIEDREVTLKRLRRRGASIALE
                     PANPAYETRIFGPHQIRVQGRLVGILRRY"
     gene            complement(1681..2874)
                     /locus_tag="P5558_13865"
     CDS             complement(1681..2874)
                     /locus_tag="P5558_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRY25446.1"
                     /translation="MLEVAAAQARITAALRPLPAETVPLQSALGRVLAADIVALRDQP
                     PAAVSAMDGYAVRAVDTTAGGWLEVIGEVAAGQPARLRVQPGQAVRIFTGGLLPPGAD
                     AILIQEDAVAEGSRVRPKEAVSPGLFARERALDYGAGWVGLSAGQRLDPRHIGLAASL
                     GAAWVEVRRQPRVGLLATGNELVLPGRSPAPHQISSSNSFTLGAMVEAWGGLPVDLGI
                     AADEPAALAARLAAATGLDLLLTTGGASVGEHDLVRQVAGERGLELDFWKIRMRPGKP
                     LIFGRIGEVPLLGLPGNPVSAAVCGIVFLRGALRCLLGLDPTLPSGTATLAEPLPGGG
                     ERQEYMRAERDGEGAIRATGRQDSSMFAALTTADVLLVRPPRDGPLERGATVTIIDLA
                     TALGA"
     gene            complement(2895..3380)
                     /gene="moaC"
                     /locus_tag="P5558_13870"
     CDS             complement(2895..3380)
                     /gene="moaC"
                     /locus_tag="P5558_13870"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRY25447.1"
                     /translation="MSDLTHIDAAGNAVMVDVGAKAETDRTATAEGRITMQLTTLQRI
                     ADGGIRKGDVLTIAQLAGIMGAKRTSDLIPLCHPLALTAVNVELEPERDGPLGPAIRI
                     TATCRLSGRTGVEMEALTAVSVAALTIYDMCKAIDRGMTIDGIRLVHKAGGRSGEFRA
                     D"
     gene            complement(3377..4180)
                     /gene="trpC"
                     /locus_tag="P5558_13875"
     CDS             complement(3377..4180)
                     /gene="trpC"
                     /locus_tag="P5558_13875"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRY25448.1"
                     /translation="MSDVLERICVAKRRDVEHRRQALPMAELERRLAGASPVRGFRQA
                     LEATIAKGRFALIAEIKKASPSKGLIREDFVPAELARAYAAGGAACLSVLTDKPSFQG
                     DDAFLAEAREAVKLPALRKDFMLEPYQILESRTLGADCILLIIAALDDPTAKELCRLA
                     RGLGMDVLAEVHDAAELDRALALDADLVGINNRNLKTLEVDIATTEALAPRVPADRLL
                     VAESGLFDHADLVRMAAVGAKAFLVGEALMREPDVSRATRRLLGLEAGT"
     gene            complement(4177..5211)
                     /gene="trpD"
                     /locus_tag="P5558_13880"
     CDS             complement(4177..5211)
                     /gene="trpD"
                     /locus_tag="P5558_13880"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRY25449.1"
                     /translation="MSSDLVRFKELIGIAACGRPLTFEEAGEAFAMMMAGDATPAQMA
                     GLLMALRVRGESIDEITAGARTLRERMTRITAPAGAIDTCGTGGDASGTFNISTAAAL
                     VVAGAGVPVAKHGNRGLSSRSGSSDVLTALGVDIEAPFPVIEHAMREAGIGFMMAPRH
                     HGAMRHVAGPRVELGTRTIFNLLGPLANPAGVRRQVVGVFALDWIEPLARVLGRLGAE
                     RAWVVHGEPGLDELSTAGTTHVAEWHEGEMRRFTVEPEPLGLARVELAALRGGDAATN
                     AAAIRAMLGGEGGPLREAVLLAAAAGLVVGGAAADLPAGIARSAEAIDSGRARACLDR
                     LVRITNEDRL"
     gene            complement(5208..5792)
                     /locus_tag="P5558_13885"
     CDS             complement(5208..5792)
                     /locus_tag="P5558_13885"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRY25450.1"
                     /translation="MLLLIDNYDSFTYNLYHYLGELGARTTVIRNDAMDVERALALRP
                     SAIVISPGPCDPDLAGISLDLIDRARTLCPILGVCLGHQAIAQVFGARVVRARTVMHG
                     KCSAIHHDGTGLFAGLPSPFRATRYHSLVAEPTTLPDELVVNARTEDGVVMGLRHRHL
                     PIHGLQFHPESIESEHGHAMLRNFLALVPLEQAA"
     gene            6038..7261
                     /locus_tag="P5558_13890"
     CDS             6038..7261
                     /locus_tag="P5558_13890"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRY25451.1"
                     /translation="MPVAGAYVMAGVLGFGMILGLAKLTTTPPSIPARSDEVAVLALP
                     VTPPAPLAEAPAATGADPEVTHEADIEIAELPPTLSGRIRLPQPTWSMRISPPPYEEH
                     LPPGVAAIEPAAPPAEAREIAGANSAPVVVSPMLAKPGRPPFVAPGLAPLVSIIIDDM
                     GYSPHSLTRLAALPGPLTLSFLPYADGTPAMLAAAGRGPFDIFLHLPMEPLGDADPGI
                     NAILADLDEAQLRKRVRQALAAVPAAVGVNNHMGSRVTALPETMRWVMEELRQQPLLF
                     VDSRTNPDSVAEGTAAMLGLPATGRDVFIDHVPEPEAIARQLALIERVARQGGSVVAI
                     GHPYDVTLRALERWLPGIERRGFRLASVADVIAYRQCGDAALDPWTCRPELQLVGTGA
                     IALPPGPAPPGKAAP"
     gene            7415..10060
                     /locus_tag="P5558_13895"
     CDS             7415..10060
                     /locus_tag="P5558_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRY25452.1"
                     /translation="MPILSRRSTGQLIMKWRHLGLAVLVLLVAAVAVMAGLLYLPQLE
                     EQRRGFVEGLLERVIGRSVVVSGPIDIRPGHVSTVTIADASLGEGGPDGWQGTLTLKR
                     LSITFDVLRAILGPFRVQRLEGDGLEIVVSGGTPAAEDSPLRLSPIIGPLARLMNAGR
                     TGEIALTDLSFERRDDPDGWNGKLTFTEMTAAESGDAEAWKLAADGALDGSALKMSAD
                     FSAVQPHPDFGQARAFTVEASLPGVDERLEGFLEANGDKIDATLTTTLSSLGDLLDAL
                     KLKRQMEATGKASMQLSGKVDALTAAKVSGELIVSTGERVTVEGRIADLSLQKGIELV
                     LQADLRRSDGSAPKPVEALDLLIESVKGQFSGALRQLTVSNLVLSTNVSSADIQKIGP
                     ISVKSLTRDDQGRLGLSGVHVLAGDPSAPSLDLNGSVADILAQTGIAFSGSFNLDVVA
                     LATGQPTPPGIGHLAGTITVADTDGKTRIESLSAALKPADIVALELDLPNAAKGEQQK
                     PATIDLGVPDLDALAKVLGGPSVGGGSASFTGEVELVDALTISGRGSVDQTAIWLDLS
                     QDVVGDRPVFKGRVKFPRLQPAGLQSLGKLASLVPDSPEGDEAQPAATTGPNLDAELE
                     VTAALLDSDAAAQGSFEGKLVYRDNKATIDPLALTYLGGKLDAKLTAETGGDAMPVTL
                     KGTIEQLELAKLIAELGGQPMVQAPLGATFELAATGADLDALGKTLDGNVAVTVGSGT
                     VGTRLIDLTGENIVSWLFSSGSSASLVCADGQITFASGLGTIQRLILETDNVQLRGTG
                     TIDLARDRLNLTFTPRPLREQLLQVVTPFRVRGPIDAPSVAVAGGTGSIAGRAVAETL
                     TLPLNVLRALLDRRGSNRVPCAADP"
     gene            complement(10082..10810)
                     /locus_tag="P5558_13900"
     CDS             complement(10082..10810)
                     /locus_tag="P5558_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRY25453.1"
                     /translation="MVDLQRVNKWYGDFHVLKDVSLTVRRGERIVVCGPSGSGKSTLI
                     RCINRLEEHQQGRIVVENIELTNDVKNIEMIRREVGMVFQSFNLFPHLTVLENCTLAP
                     LWVRKMPQKEAAAIAMKFLERVRIPEQAQKYPGQLSGGQQQRVAIARSLCMMPQVMLF
                     DEPTSALDPEMIKEVLDVMVELAHEGMTMICVTHEMGFAKSVADNVVFMDQGEIVEMA
                     PPAEFFTNPRSDRTKAFLSQILAH"
     gene            complement(10876..11982)
                     /locus_tag="P5558_13905"
     CDS             complement(10876..11982)
                     /locus_tag="P5558_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY25454.1"
                     /translation="MEETLTRDAQTPARARPRGPRDPIGWARANLFDGWFNSLLTILI
                     VALLVWIIPPFIRWAFTDAVLGHATPDECKAATGACWAVVTEKYRLILFGRYPFDEQW
                     RPLFGIIALVGMALLSCDRRFWGWQLLVIWALGLGSFFVLMGGGVLGLSSVRTNLWGG
                     LPLTLMLAFIGIAAAFPLAILLALGRRSKLPIISALSVGYIELIRGVPLITLLFMGSF
                     MLPLFMPTGVDIDAILRAQVAIIMFAAAYLAEVVRGGLQALPKGQHEAAAALGLGYWQ
                     TQGLIILPQALKITIPPIVNTFIGLFKDTSLVAIVSLTDLVLATRQALGDPEWRQFFI
                     EAYLFIMLIYWVFCFFMSKYSQHLERSLATDRRS"
     gene            complement(11986..13185)
                     /locus_tag="P5558_13910"
     CDS             complement(11986..13185)
                     /locus_tag="P5558_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY25455.1"
                     /translation="MPREGLDRTTARAASSGVVWWRNPTIRGVLAQALVVVLLLAVVW
                     YLVHNTLTNLNERSIRTGFSFLDQEAGFAIGESLIAFAPTDTYGRALLVGLLNTLRVS
                     IIGIVLATLIGVVVGIARLSSNWLVARLASVYVEVTRNIPLLLQLFFWYTIVTQLLPA
                     VREALEPLPGVVLSKSGLQFPIIVGDPAHLPVLGVLALSVVAAFVYRHRAYRRQLETG
                     QEPRLLLPMLAIICLPALAVFLVTGAPLDLDVPEFTRFNYRGGASVTPEFLALLLGLS
                     IYTAGFIAEIVRSGILAVPWGQTEAAAALGFRRSLQLRLIVLPQALRIIVPPTTSNYL
                     NLTKNSSLAVAIGYPDLVSVGNTTLNQTGQAIECISIMMAIYLTISLLISAFMNWYNR
                     RIALVER"
     gene            complement(13288..>13881)
                     /locus_tag="P5558_13915"
     CDS             complement(13288..>13881)
                     /locus_tag="P5558_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY25456.1"
                     /translation="DGAEICVQPGTTTELNLADYFRANNMDFSAVVIEGFEQSLETFF
                     SGRCQAYTTDKSGLAAIIANDAPNPDDYVILPETISKEPLGPAVRRGDDEWFAIAKWV
                     LFAAIQAEESGVSSANVDEMMESTDPNVQRLLGVSDQLGEKLTIANDWAYQVIKQVGN
                     YGEMYDRNVGPETPLKLERGQNALWTKGGLMYAPPLR"
BASE COUNT         2225 a         4828 c         4750 g         2078 t
ORIGIN      
        1 gtgcccgccc ctgcacgcga gactagccgc gcctcgcttt ggtcgctagg tggtgacgcc
       61 tgacgggacc acggacacca ctcggatgcg caccctcgac gtacgtctgg cgccagtgat
      121 cgcccatcgc ggcgcctcgg ccgacacgcc ggagaatacc cttgctgccc tccgcgccgc
      181 ggcggcggcc ggtgcccggt gggtcgagtt cgacgtcaag ctcagccgtg acggctactg
      241 catcctcatg cacgacgacc tcctcaaccg gaccaccaac ggccgcggcg aggtggcgca
      301 ttacgacctc gacgagctgc gccggctcga cgccgggggc tggttcggcc gccgcttccg
      361 tggcgagccc gtgccgaccc tccaggaagc gctggccctc tgcgtcgagc tcgacctcga
      421 catcaatctg gagatcaagc cctgccccgg ccggcagcac gagacggcca tccgggtggt
      481 cgacgagctg cgtcgccact ggcccgccgg ccgatcatgg ccactgatct cgagcttcgc
      541 gatcgcatcc ctggaggagg tccgcaagct cggccccgat atgccgcacg gtctgctcat
      601 cggccgaccg accagacgcg ctctcggcat gctcgaccgc ctcgaatgca gcacgctgca
      661 ctgcgacgcc aagcatgcct cggcggagct cgtccggcat ctgaccagga gcggccggcc
      721 gcttctctgc tacaccgtca acgacgcgag ccgggcgctc cgcctcctgg agatgggggt
      781 gcgcgcggtt ttcaccgacc gaccccagct gatcgccgaa gcgatcgctg cccgcagcgc
      841 gaagcagggt cagtagcggc gcaggatgcc gaccagccgg ccctgcaccc ggatctggtg
      901 cggcccgaag atcctggtct cgtaggccgg attggccggc tccagcgcga tcgaggcgcc
      961 ccgccggcgc aaccgcttga gcgtcacctc gcggtcctcg atcagggcca cgacgatgct
     1021 gccgttctcc gaggtctcgc accgctggat gaccgcatag tcgccatcga cgatgcccgc
     1081 atcgaccatc gaatcgccgc tcacctggag cacgtagtgc tcgccctcgc cgagcagcag
     1141 gcccggcacg tcgatcgccg tcgactgatc gagaagagcc tcgatcggcg cgcccgcggc
     1201 gatcctgccg accagcggca aggagcggct gcccgattcg ctgaccggcg tcgcccgtgg
     1261 cccggcgccg aagcggccgc gcacgatatt gctcgacggg cgcgcccgac ccactgcgtt
     1321 cgcgccggac ccggcggccg ccgccggccc ctctgtcgaa aagtcggcca gcggcccgcc
     1381 gtgcgggcct cgcacgatct cgagcgcgcg tgcccgataa gccaggcgac ggatatagcc
     1441 gcgttcctcg aggccggaga tgagacgatg cacacctgat ttggacttca actgcagcgc
     1501 gttccgcatc tcttcgaacg acggcggaac ctgatgctcg acgacatagg aatggatgta
     1561 ggtcaacagt tgcagttgtc gttgggtgag caacatccat ctccctggcg gcagccggca
     1621 cggctttgtt ctatttaagt tctcatgcct gtcaagcgcc ttgccgggtg gtcgccaccc
     1681 tcaggcgccg agcgcggtgg cgagatcgat gatggtgacg gttgcacctc gctccagtgg
     1741 cccgtcgcgg ggcggacgga cgagcaggac atcagccgtg gtcagtgctg cgaacatcga
     1801 gctgtcctgg cgcccggtgg cccggattgc accctctccg tcccgctcgg cccgcatgta
     1861 ctcctggcgc tcgccaccac cgggcagcgg ctcggcgagc gttgctgtgc cgctcggcag
     1921 ggtcggatcg aggccaagga ggcaccgaag tgccccgcgc aggaagacaa tgccgcatac
     1981 cgcggccgag accgggttgc ccggcaggcc cagcagcggc acttcgccga tccgcccgaa
     2041 gatcagaggc ttgcccggcc gcatccggat cttccagaaa tccagctcga gtccgcgctc
     2101 accggcaacc tgccgcacga ggtcgtgctc gccgaccgag gcgccgccgg tcgtcagcag
     2161 gagatcgagg ccggtggcag cagcgagcct ggccgccagg gccgcgggct cgtccgcggc
     2221 gatgccgaga tcgaccggca ggccacccca cgcctcgacc attgccccca gggtgaagct
     2281 gttggagctg gagatctgat gcggtgccgg actcctgccg ggcaggacca gctcgttgcc
     2341 ggtcgccagg aggccgaccc ggggctggcg ccgcacctcg acccaagcgg cgccgagcga
     2401 tgcggcgagg ccgatatgcc gcgggtcgag ccgctggccg gcagagaggc caacccagcc
     2461 ggcgccatag tcgagcgccc gctcgcgcgc gaagagaccc ggcgagacgg cctcctttgg
     2521 ccgcacccgg ctgccctcgg cgaccgcatc ctcctggatc agaatcgcgt ccgcaccggg
     2581 cggcaacagc ccgccggtaa aaatccgcac cgcctgcccc ggctgcaccc tgagccgggc
     2641 gggctgcccg gccgccacct cgccgatgac ctcgagccag ccgccggccg tcgtatcgac
     2701 ggcgcgtacg gcgtagccgt ccatggccga caccgccgcc ggcggctggt cgcgcaacgc
     2761 cacgatgtcg gccgcgagca cccgccccag cgccgactgc agcggcacgg tctcggccgg
     2821 gagcggccgc agggccgccg tgatccgcgc ctgggcggcc gcaacctcga gcatgtcaga
     2881 ccaccccccg ccgctcagtc cgcacggaac tcgcccgagc ggccgccagc cttgtggacg
     2941 aggcggatgc cgtcgatcgt catgccgcgg tcgatcgcct tgcacatgtc gtagatggtg
     3001 agtgcggcga cgctgacggc ggtcagggcc tccatctcga ccccggtccg gccactgagc
     3061 cgacaggtgg cggtgatacg gatcgccggc ccgagcggcc cgtcgcgctc cggctcgagc
     3121 tcgacgttga cggcagtcag cgccagcgga tggcagagcg ggatgaggtc tgatgtgcgc
     3181 ttcgccccca tgatcccggc gagctgggcg atggtcagca catcgccctt cctgatgccg
     3241 ccgtcggcaa ttcgctgaag ggtcgtaagc tgcatcgtga tccgcccctc ggcggtggcg
     3301 gtccggtccg tctcggcctt ggcgccgaca tccaccatga cggcgttgcc ggccgcatcg
     3361 atatgcgtca ggtcgctcat gtcccggctt cgaggccgag caggcggcgc gtggcccggc
     3421 tgacatcggg ttcgcgcatc agcgcctcgc cgacgagaaa ggccttggca ccgacagcgg
     3481 ccatccgcac cagatcggca tggtcaaaga gcccactctc ggcgacgagc agccggtcgg
     3541 ccgggacgcg cggcgccagc gcctccgtcg ttgcgatatc gacctcgagc gtcttgaggt
     3601 tgcggttgtt gatgccgacc aggtcggcgt cgagcgccag cgcccggtcg agctcggccg
     3661 cgtcgtgcac ctcggccagc acatccatcc cgagcccccg ggcgagccga cagagctcct
     3721 tcgccgtagg gtcatcgagg gccgcaatga tcaaaaggat gcagtccgcc cccaaggtcc
     3781 ggctctcgag gatctggtag ggctcgagca tgaagtcctt gcgcagggcc ggcagcttca
     3841 cggcttcccg ggcctcggcg agaaaggcgt cgtcgccctg gaagctcggc ttgtcggtca
     3901 gcaccgaaag gcaggcggcg ccgcctgccg cataggctcg ggccagctct gccggcacga
     3961 aatcttcccg gatcagcccc ttgctcggcg aagccttctt gatctcggcg atgagggcga
     4021 agcggccctt cgcgatcgtt gcctccagag cttgcctgaa gccccggacc ggcgacgcgc
     4081 cggccaggcg acgctcgagc tcggccatcg gcagcgcctg gcggcggtgc tccacgtcgc
     4141 gtcgcttcgc gacgcagatc cgctcgagga catcgctcat agccggtcct cgttggtgat
     4201 gcgcaccagc cggtcgagac aggcccgcgc ccggccgctg tcgatcgcct cggccgagcg
     4261 ggcgatcccc gccggcaggt cggcggcggc gccgccgacc accagcccgg cggcggcggc
     4321 gagcaggacc gcctcgcgca gggggccgcc ctcgccgccc agcatggcgc ggatcgcggc
     4381 cgcattggtc gcggcgtcgc cgcccctgag tgccgcgagc tcgaccctgg cgaggccgag
     4441 cggctccggc tcgacggtga agcggcgcat ctcgccttca tgccattcgg cgacgtgcgt
     4501 tgtgccggcc gtgctcagct cgtcgagtcc cggctcgcca tgcacgaccc aggcccgctc
     4561 cgccccgagc cgcccgagca cgcgcgccag cggctcgatc cagtcgagcg cgaagacgcc
     4621 caccacctgc cgccggaccc cggccgggtt ggcgagcggg ccgagcaggt tgaagatggt
     4681 gcgcgtgccg agctcgaccc tggggccggc cacgtggcgc atggcgccat ggtgcctggg
     4741 cgccatcatg aagccgatgc cggcctcgcg catggcatgc tcgatgaccg ggaagggcgc
     4801 ctcgatgtcg acgccgagcg ccgtcagcac gtccgacgag ccggagcgcg aggacaaccc
     4861 gcgattgccg tgcttggcga ccggcacacc ggcgccggca acgaccaggg ctgcggccgt
     4921 cgagatgttg aaggtgccgc tggcgtcgcc gcccgttccg caggtgtcga tggcgccggc
     4981 cggggcggtg atgcgggtca tgcgctcgcg cagcgtccgg gcaccggccg tgatctcgtc
     5041 gatgctctcg ccgcgcaccc gtagcgccat gaggagcccg gccatctggg ccggcgtggc
     5101 atcacccgcc atcatcatgg cgaaggcttc ccccgcctcc tcgaaagtca gcggtcggcc
     5161 gcaggcggcg atgccgatca gctccttgaa gcgaacgaga tcggagctca agcggcctgc
     5221 tccagcggaa ccaaagcgag gaagttgcgc agcatggcgt ggccgtgctc gctctcgatg
     5281 ctttcggggt ggaactgcag cccgtggatc ggcaggtgac ggtggcgcag gcccatgacg
     5341 accccgtcct cggtgcgggc gttgaccacg agctcgtcgg gcagagtcgt cggctccgcc
     5401 accagcgaat gatagcgggt ggcgcggaag ggcgacggca acccagcgaa gagacccgtg
     5461 ccgtcatgat ggatggcgct gcacttgcca tgcatcaccg tccgcgcccg cacgacccgg
     5521 gcgccgaaga cctgggcaat cgcctgatgg ccgaggcaca cgccgagaat cgggcatagc
     5581 gtgcgcgccc ggtcgataag gtcgaggctg atgccggcca gatcgggatc gcatggtccg
     5641 ggcgagatca cgatggccga cgggcgaagc gccagcgccc gttcgacgtc catcgcgtcg
     5701 ttgcggatca ccgtcgtgcg ggcgccgagc tcacccaggt agtggtagag attgtaggtg
     5761 aagctgtcgt agttatcgat caggaggagc atggcgggac cgatcgggct ggcgtctcgg
     5821 gaacagcgag aaaagcccca gcccaggcta ccgtcaagcc ccgcgcggcg cagcgccggc
     5881 aagggtgcat gcggcacggg aatcaggtcg tggttcaact gtggccggtt ttcaattagc
     5941 ttacccgagg gcgcggcagc gggcgacgga taaccatggg tgagcaggct tgaggcggct
     6001 gaaccgccgg gggccgcggt cgcgtcggct gcgtccgttg ccggttgccg gcgcctatgt
     6061 catggcgggg gtgctcggct tcggcatgat cctcggcctc gcgaagctca cgaccacccc
     6121 gccgtcaatt cccgcccgat ccgatgaggt cgcggtgctg gcgttgccgg tcacgccccc
     6181 agcccccctg gcggaggcgc cggcggcaac gggagcagat ccggaggtca cgcacgaggc
     6241 ggacatcgag atcgccgagc tgccgccgac cctgtccggg cgtatccgcc tgccgcagcc
     6301 gacctggtcg atgcggatca gcccgccgcc atacgaggag cacttgccgc ccggcgtcgc
     6361 cgccatcgag cccgccgccc cgccggccga ggcccgggag atagccgggg cgaacagcgc
     6421 gccggtggtc gtgtcgccga tgttggcgaa gccgggaagg ccgccattcg tggcgcctgg
     6481 cctagcgcca ctggtcagca tcatcatcga cgacatgggc tacagcccgc attcgcttac
     6541 ccgcctcgcc gccctccccg gcccgctcac cctgtcgttc ctgccctatg ccgatggcac
     6601 gccggccatg ctggccgccg ccgggcgagg cccgttcgac atctttctcc atctgcccat
     6661 ggaaccactg ggtgacgccg atcccggcat caatgccatc ctcgccgatc tcgacgaagc
     6721 ccagctccgc aagcgcgtcc gccaggcgct ggctgccgtg ccggctgccg tcggcgtcaa
     6781 caaccacatg ggcagccggg tcacggcgct gccggaaacc atgcgctggg tcatggagga
     6841 gttacggcag cagccgctgc tcttcgtcga cagccggacc aatcccgaca gcgtcgccga
     6901 agggaccgcc gccatgctcg gcctgccggc caccggccgc gacgtgttca tcgaccatgt
     6961 ccccgaaccg gaagcgatcg cacgccagct cgcgctgatc gagcgggtcg cccgccaggg
     7021 cggcagcgtc gtggccatcg gccatcccta cgacgtgacc ttgcgggcgc tcgagcgctg
     7081 gctccctggc atcgagcggc gcggctttcg cctggccagc gtcgccgacg tcatcgccta
     7141 tcggcaatgc ggcgatgccg ctctcgatcc ctggacctgt cgccccgagc tgcagctcgt
     7201 cggcaccggg gcgattgccc tgccacccgg cccagctccg ccggggaagg cggcgccctg
     7261 attcatcgct cgagctccgg cgatcggccg tccctttcaa tcggccgata ccagacctat
     7321 acttttcctc cgcgaccggc ggggcatcgt cgcgaagcat cggccgcgag tggcgtcggc
     7381 gcgccgggac gtgcgatccg ttcgtgctcg cgccatgccc atcctcagca ggcgctccac
     7441 cgggcagctg atcatgaaat ggcgccatct cggccttgcc gttctcgtcc ttctcgtggc
     7501 ggctgtggcc gtcatggccg ggctcctcta tctgccgcag ctcgaggagc agcgtcgcgg
     7561 cttcgtcgag ggattgctgg agcgcgtcat cggtcgctcg gtcgtggtct cgggcccgat
     7621 cgacatccgg cccggccacg tgtccacggt cacgatcgcc gacgcctcgc tcggcgaggg
     7681 tggccccgac ggctggcagg gcacgctgac gttgaagcgg ctgagcatca cgttcgatgt
     7741 cctgcgtgcc atcctcggcc ccttccgggt ccagcgtctg gagggcgatg gcctcgagat
     7801 cgtggtatcc ggcggcacac ccgccgcgga agacagcccc ttgcgcctga gcccgatcat
     7861 cggacctctg gcccgcctga tgaacgccgg tcgcaccggc gagatcgccc tcaccgacct
     7921 ctcgttcgaa cgtcgcgacg accccgacgg ctggaacggc aagctcacct tcaccgaaat
     7981 gactgccgcg gaaagcggcg atgcggaagc ctggaagctc gcggccgatg gcgcgctcga
     8041 cggctcggcg ctgaagatgt ccgccgattt ctcggcggtg caaccgcatc ccgacttcgg
     8101 ccaggcgcgg gctttcaccg tggaagcctc cctgcctggc gtggacgagc gcctggaagg
     8161 ctttctcgag gccaatggtg acaagatcga cgccacgctg accaccacgc tgagctcgct
     8221 cggcgacctg ctggatgcgc tgaagctgaa gcggcagatg gaagcgaccg gcaaggcgag
     8281 catgcagctc tccggcaagg tggatgcgct caccgccgcc aaggtgagcg gggagctgat
     8341 cgtctcgacc ggcgagcgcg tcacggtcga gggcagaatc gccgatctgt cgctgcagaa
     8401 gggtatcgag ctcgtccttc aggccgatct gcgacgctcg gacggatcgg cgccaaagcc
     8461 cgtcgaagcc ctcgatctcc tgattgaatc ggtcaagggc caattctcgg gggcgctgcg
     8521 ccagctcacc gtcagcaacc tcgtattgag caccaacgtc agctcggccg acatccagaa
     8581 gatcgggccg atcagcgtca agtccctgac gcgcgacgat cagggccgcc tcgggctcag
     8641 cggggtccat gtcctggccg gcgatccgag tgccccgagc ctcgatctga acggcagcgt
     8701 ggccgatatc ctcgcccaga ccggcatcgc gttctccggc agcttcaatc tcgatgtggt
     8761 ggccctggcg accggccagc cgactccgcc cggaatcggc cacctcgcgg gcacgatcac
     8821 ggtggccgac acggacggga agacgcgcat cgagagcctt tcggcggccc tcaagccggc
     8881 cgacatcgtc gccctcgagc tcgacctgcc gaacgccgcg aagggggagc agcagaagcc
     8941 ggcgaccatc gacctcggcg ttcccgacct cgatgccctg gcgaaagtgc tcgggggacc
     9001 atcggtcggc ggcggcagcg ccagcttcac gggcgaggtc gagctggtcg atgccctcac
     9061 catatccggc cgcggctctg tggatcaaac ggcgatctgg ctggacctga gccaggacgt
     9121 cgtcggcgat cgacccgtct tcaaaggcag ggtcaagttc ccacgcctgc agccggcggg
     9181 cctgcagagc ctgggcaagc tcgccagcct cgtgccggac tcgcccgagg gggatgaagc
     9241 gcagccggcc gcgacgaccg gacccaatct cgacgccgag ctggaagtga cggcggcgct
     9301 cctcgacagc gacgccgcag cacagggatc attcgagggc aagctcgtct atcgcgacaa
     9361 caaggccaca atcgatcccc tggcgctgac ctatctcggc ggcaagctcg acgcaaagct
     9421 gaccgccgaa accggtggcg acgccatgcc ggtcactctg aagggcacga tcgagcagct
     9481 cgagctcgcc aagctgattg ccgaattggg cggccagccc atggtccagg cgccgctcgg
     9541 cgccaccttc gagctcgccg cgaccggcgc cgatctcgat gcgctgggca agacgctcga
     9601 cggcaacgtc gcggtcacgg tcggcagcgg cacggtcggc acccgactga tcgacctcac
     9661 cggcgagaac atcgtcagtt ggctcttctc ctccggctcc agcgccagtt tggtctgtgc
     9721 cgatggccag atcaccttcg cctcgggcct cggcaccatc cagcggctga tcctggagac
     9781 ggacaacgtc cagttgcgcg gcaccggcac gatcgatctg gctcgcgacc ggctgaacct
     9841 caccttcaca ccgcgcccgc tgcgcgagca gctcctccag gtagtgacgc cctttcgcgt
     9901 gcgcggcccg atcgacgcgc cgagcgtcgc ggtcgccggc ggcacgggca gcatcgccgg
     9961 ccgggccgtg gccgagacgc tcacgctccc cctcaatgtg ctccgggcat tgctcgaccg
    10021 ccgcggcagc aacagagtgc cctgcgccgc cgatccatga gccgcacctg cgtcgtgtgt
    10081 ctcaatgcgc caggatctgg ctgaggaacg ccttggtgcg gtcggagcgg gggttggtga
    10141 agaactcggc cgggggcgcc atctcgacga tctcgccctg gtccatgaag acgacgttgt
    10201 cggccaccga cttggcgaag cccatctcgt gggtgacgca gatcatggtc atgccttcgt
    10261 gcgccagctc gaccatcacg tcgagcacct ccttgatcat ctcgggatcg agggcggagg
    10321 tcggctcgtc gaacagcatc acctgcggca tcatgcagag cgagcgtgcg atcgccacgc
    10381 gctgctgctg accgcccgag agctggcccg gatatttttg tgcctgctcg gggatgcgca
    10441 cgcgctcgag gaacttcatg gcgatcgccg cggcctcctt ctgcggcatc ttgcggaccc
    10501 acaagggggc cagcgtgcag ttctcgagca cggtgagatg cggaaagagg ttgaaggact
    10561 ggaacaccat gccgacctcg cggcggatca tctcgatgtt cttgacgtcg ttggtgagct
    10621 cgatgttctc gaccacgatg cgaccctgct ggtgctcctc gagcctgttg atgcagcgga
    10681 tcagcgtcga cttgccggag cccgacgggc cgcagacgac gatccgctcg ccccggcgca
    10741 cggtcaggct cacgtccttc aggacgtgaa agtcgccgta ccatttgttg acgcgctgca
    10801 ggtcgaccat gatctcgtcg ctgcgcccgg cggcttcggc attggcggtg gcatgggcca
    10861 tgaaacttcg tcctctcaac tgcggcggtc ggtggcgagg cttcgctcga gatgctggct
    10921 gtacttcgac atgaagaagc agaagaccca gtagatcagc atgatgaaga gataggcctc
    10981 gatgaagaac tggcgccact cggggtcgcc cagcgcctgg cgcgtcgcca gcacgaggtc
    11041 ggtcaggctg acgatggcga cgagcgacgt atccttgaag aggccgatga aggtgttgac
    11101 gatcggcggg atggtgatct tcagcgcctg cggcaggatg atcaggccct gggtctgcca
    11161 gtaccccagc ccgagcgccg cggccgcctc gtgctgcccc ttgggcaggg cctgcaggcc
    11221 gccgcggacg acctcggcga ggtaggcggc ggcgaacatg atgatggcga cctgggcgcg
    11281 caggatggcg tcgatgtcga ccccggtcgg catgaagagc ggcagcatga aggagcccat
    11341 gaagagcagg gtgatcagcg gcacgccgcg gatcagctcg atatagccga ccgacaaggc
    11401 gctgatgatc ggcagcttcg agcgccggcc gagggcgagc aggatggcga gcggaaaggc
    11461 cgcggcgatg ccgatgaagg cgagcatcag cgtcagcggc aggccgcccc agagattggt
    11521 gcggaccgaa ctcaggccga gcacgccgcc acccatgaga acgaagaagc tgccgaggcc
    11581 gagcgcccag atcaccagaa gctgccagcc ccagaaccgc cggtcgcagc tcaagagcgc
    11641 catgccgacc agggcgatga tgccgaagag cggccgccac tgctcgtcga agggatagcg
    11701 gccgaagagg atgagccggt atttctcggt cacgaccgcc cagcaggcgc ccgtggcggc
    11761 cttgcactcg tccggcgtgg catgaccgag gaccgcgtcg gtgaacgccc agcgaatgaa
    11821 gggcggaatg atccagacga gcagggcgac aatgaggatg gtgagcaggc tgttgaacca
    11881 gccatcgaag agattggctc tcgcccaacc gatggggtcg cggggcccgc gcggccgggc
    11941 gcgggccggc gtctgggcat cgcgcgtgag ggtctcttcc atggctcagc gctccaccag
    12001 cgcgatgcgc cggttgtacc agttcatgaa ggccgagatc agcaggctga tggtgaggta
    12061 gatcgccatc atgatgctga tgcactcgat cgcctggccg gtctggttca gcgtcgtgtt
    12121 gccgaccgag acgaggtccg gatagccgat cgccaccgcc agcgagctgt tcttggtgag
    12181 gttgaggtag ttgctggtgg tcggcggcac gatgatgcgc agggcctgcg gcagcacgat
    12241 gaggcgaagc tgcaggctgc ggcgaaagcc cagcgctgcc gccgcctcgg tctgtcccca
    12301 gggcaccgcg agaatgccgc ttctgacgat ctcggcgatg aagccggccg tgtagatgct
    12361 cagccccagg aggagcgcga ggaactccgg ggtgacgctt gccccgcccc gatagttgaa
    12421 gcgggtgaac tcgggcacat ccagatcgag cggggcgccg gtgacgagga aaaccgccag
    12481 ggccggcagg cagatgatcg ccagcatcgg cagcagcagc cgcggctcct gcccggtctc
    12541 gagctggcgt cgataggcgc ggtgccggta gacgaacgcg gccacgacgg agagcgccag
    12601 cacgccgagg acgggcagat gcgccgggtc gccgacgatg atggggaact gcagcccgct
    12661 cttgctcagc accacgccgg gcagcggctc cagggcctcc cgcacggccg gcaggagctg
    12721 ggtcacgatc gtgtaccaga agaagagctg cagcaggagc gggatgttgc gggtcacctc
    12781 gacatagacc gaggccagcc tggcgacgag ccagttgctg ctgaggcggg caatgccgac
    12841 gacgacgccg atcagggtcg cgagcacgat gccgatgatc gagacgcgga gcgtgttgag
    12901 gagcccgacg agcagggccc ggccataggt gtcggtcggc gcgaaggcga tcagcgattc
    12961 gccgatggcg aagccggcct cctggtcgag gaagctgaag ccggtgcgga tgctccgctc
    13021 gttcaggttg gtgagcgtgt tgtggacgag gtaccagacg acagcgagga ggagcaccac
    13081 gaccagcgcc tgggcgagca cgccacgaat ggtcgggttg cgccaccaga ccacgcccga
    13141 cgacgccgcc ctggcggtgg tgcggtcgag cccttcacgc ggcatgttcg tgagcccctc
    13201 gcgctgtccc ctgaaagatc gccggaggcc acccgcggca cgcccggcgg acagaggtcc
    13261 acctgccggg catgccgcgg gcccggctca tcgcagcggc ggcgcgtaca tcagcccgcc
    13321 cttggtccag agcgcattct gcccgcgctc cagcttcagc ggcgtctcgg ggccgacatt
    13381 gcggtcgtac atctcgccgt aattgccgac ctgcttgatc acctggtagg cccagtcatt
    13441 ggcgatggtc agcttctcgc cgagctgatc ggagacaccg aggagccgct gcacgttggg
    13501 atcggtgctc tccatcatct cgtcgacatt ggccgagctg acaccgctct cctcggcctg
    13561 gatggcggca aacagcaccc atttcgcgat cgcgaaccac tcgtcgtcac cgcgccgcac
    13621 ggccgggccg agcggctcct tggagatcgt ctcgggcagg atgacgtagt cgtcgggatt
    13681 gggcgcgtcg ttggcgatga tggccgcgag gcccgacttg tcggtggtgt aggcctggca
    13741 acgacccgag aagaaggtct ccagcgactg ctcgaagccc tcgatcacca cggccgagaa
    13801 gtccatgttg ttggcacgga aatagtcggc gaggttgagc tcggtcgtgg tgcccggctg
    13861 cacgcagatc tcggcgccgt c
//
LOCUS       DAOTFI010000129        13869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5292_length_13869_cov_6.580353, whole genome shotgun sequence.
ACCESSION   DAOTFI010000129 DAOTFI010000000
VERSION     DAOTFI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5292_length_13869_cov_6.580353"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1283
                     /locus_tag="P5558_13920"
     CDS             102..1283
                     /locus_tag="P5558_13920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRY25457.1"
                     /translation="MPRLDRAYKVAEMRRQARRVLPRPVFDFADGGAEDERTLGRNEA
                     AFEDVSLLPRPLEGAAERDLSIELFGRRLSMPLMIGPTGLAGLFWPGGELCAARAATA
                     AGTAYCLSHGSVCTLEALAETGAAPRWMQVFVYRDRGFTRELTDRAAAAGYDALVLTI
                     DNQLLGKRERDIVNGFGIPPRFAPLDLLGMATRLPWLWRMRDVLRTLTFGNYVRAGRP
                     SDIRTLAGSMAGLLDPAMSWRDVEALRQHWQGPLVLKGVLHPDEARRAIEHGIDGIIV
                     SNHGGRQLDGAAASLDALPRIVEAVEGRMAVLLDGGVRRGSHVLKALALGATCCLIGR
                     PQLWGLAVGGEAGVARVLELLRQELDRAMGLCGVGRIADIGPELLAGPGNAIIPKDRR
                     T"
     gene            1280..1981
                     /locus_tag="P5558_13925"
     CDS             1280..1981
                     /locus_tag="P5558_13925"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25458.1"
                     /translation="MIDEVPLLTIRRRFSRPSADQVAAFQGLPTGFVADALLAARGGG
                     KSALDGRVKPIAESGPFCGVVVPCEAGPADNLAVFGALDIAQPGDVVIAGTDFFTEAA
                     IIGDLLLGMLQNKGVVAFVTDGFVRDIVGLRAVGLPCYASGVTPNSPAKNGPGTVGLP
                     VQVGGVLVGPGDIAVGDEDGVVVVPHAMIAATIDKLPAIKAAEATMDARVKGGLKQPD
                     FWAALSGSGRFREVD"
     gene            complement(2000..2296)
                     /locus_tag="P5558_13930"
     CDS             complement(2000..2296)
                     /locus_tag="P5558_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25459.1"
                     /translation="MSRFLQQAPTSEPGDSTNDPGKTTTEPEESTNEPAFSPTNPAAA
                     KPLSQPAFSLLVQLLSAAGRPGNREMPSEPETGLPSRAACARNTILSPFQGTTG"
     gene            2580..2972
                     /locus_tag="P5558_13935"
     CDS             2580..2972
                     /locus_tag="P5558_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRY25460.1"
                     /translation="MAELTGGCLCGKVRIVASGRPYRVGLCHCLDCRKHHGALFQATA
                     IFPEEAVTIEGETRDYAGRCFCPRCGSSVFARTGDEIEVSLGCLDSPDQLMPTYESWI
                     VRREAWLPPFPLRRSYVRDREATGRFET"
     gene            complement(2976..3452)
                     /locus_tag="P5558_13940"
     CDS             complement(2976..3452)
                     /locus_tag="P5558_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25461.1"
                     /translation="MAESDRSIFRDTAFFVALLATAMALGGALAHAYALPNKIVMARD
                     AYFTAQAIYLGWNRLAIVLAVQLLGILWVLYLHRRQGRVARPTLVALASLIAAQVVFW
                     VWTFPANRATESWTVQPETWEALRAQWEYSHLAGAGFQLLAMCALILAVLRRRDRA"
     gene            complement(3527..5041)
                     /gene="trpE"
                     /locus_tag="P5558_13945"
     CDS             complement(3527..5041)
                     /gene="trpE"
                     /locus_tag="P5558_13945"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRY25462.1"
                     /translation="MAFFEEVLPPRAAFRSAYEAGRSQVVWTTLVADLETPVSAMLKL
                     TDGRPYSFLLESVEGGAVRGRYSIIGTKPDLVWRCHGKQAEINRRCRIDRLDFQPEKL
                     PTLESLRSLLAETRIDVPDELPPMAAGLFGFLAYDMIRLVEPVPETNPDRIGLPDAVF
                     LRPTLIAIFDNIKDIVTIVTPVYPEAGLDADRAYDLALERLADAVADMARGIGPAQPA
                     HAGPPPEAVSNMTREGYHRMVERAKAYILAGDIFQVVPSQRFTVPFTLPPMALYRALR
                     RLNPSPFLFLLDLDGATLVGSSPEILVRVRDGRVTVRPLAGTRRRGHDAAEDVALAEE
                     LLADPKELAEHLMLLDLGRNDVGRVAEIGSVEVTERMIVERYSHVMHIVSNVEGNLRP
                     DCDVIDALLAGFPAGTTSGAPKVRAMQIIEELEPERRVFYAGAVGYLSADGNMDTCIA
                     LRTALVKDGTMYVQAGGGVVADSDPEAEYQESCNKARALIRAAEEAVRFASRGR"
     gene            complement(5058..6947)
                     /locus_tag="P5558_13950"
     CDS             complement(5058..6947)
                     /locus_tag="P5558_13950"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRY25463.1"
                     /translation="MLDAMRKRANSWFVRALLLVLIASFAVWGIGDTFFGRQDIAIAV
                     QVGDIEVPLQEVDRAFEIDRRAFGEQFGATLDRRQAASLGILNRSLQNVIARALVDQH
                     RRDLGIGVSDAEVAAAIRNDQRFATDGNLDRARFDMYLRSLGLGEAQYVEQVRADIGR
                     ARVLDALTGSIAAPSVLVERLGAWRGETRSGTLLEVASDAMTAPEPTDAELEAFLEEN
                     IDAFQAPEYRTVTLVTLTADDIVDEIEIDEARLREEYEARADFYTTPERRRVGQLLAS
                     DRETIEEARLALEEGAVFAELADSMADRGLTYGPLGPTAREDLPPEFADAIFALGPDE
                     VSEPVQSAFGWHLFRLIEVEPETVRSFEDARDEIRRDLALDRAIDQLPDLASALDDGI
                     AAGESLEEAALAVGTTARRFQAIDAQGRDATGAPVEGGAPSPEILAAIFAAPLEETSL
                     LEETPGGTFYMFRVDASEPSRPRRLDEVRDDVAALWRQEERQRLATERVEAIMADARS
                     GRTLETIAAGLGDEVTLRRFGPIGRAADGSEAGLTPEAVEALFATDKGNLAREPVATR
                     NGSAILRTDAIMVPEGDQPELREQLTAALRNDVLIQYEAALRRRYPVEVNEAAIASLF
                     PADEF"
     gene            7114..7866
                     /gene="tpiA"
                     /locus_tag="P5558_13955"
     CDS             7114..7866
                     /gene="tpiA"
                     /locus_tag="P5558_13955"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRY25464.1"
                     /translation="MPLARPVVLGNWKMNGLRADGLALAGRLDARAAHASGTLGIFPP
                     ATLVAAVAQRLARSRVLVGGQDCHPAESGAFTGSISAAMLADAGARAVIVGHSERRHG
                     LGETDAEVKAKGEAALAAGLMVVLCIGETEAEYVAGERHAVLVRQLDGSLPSGATAER
                     LVVAYEPVWAIGTGRTPSLAEIARTHAELRGLLAGRLADGAEVALLYGGSVKPDNAAD
                     IMAIEAVDGALVGGASLDADSFWSIYAAGGGT"
     gene            7898..8236
                     /gene="secG"
                     /locus_tag="P5558_13960"
     CDS             7898..8236
                     /gene="secG"
                     /locus_tag="P5558_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRY25465.1"
                     /translation="MTTILLVIHLLIALALVGVVLIQRSEGGALGGLGGTAMGGVMSG
                     RGTANLLTRATAILATGFMVMSLLLAYVASGGSGPVSILDGPNPFEPAPVTEPAEPAP
                     PAAPAAPVAN"
     gene            8333..9961
                     /locus_tag="P5558_13965"
     CDS             8333..9961
                     /locus_tag="P5558_13965"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRY25466.1"
                     /translation="MPRFVFITGGVVSSLGKGLAAAALGSLLQLRGYRVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVPARASDSVSSGRIYWTVLTRE
                     RHGDYLGGTVQVIPHVTNTIKEYIQKDVEDADIVLCEIGGTVGDIEGLPFLEAIRQLA
                     NELPRGEVAFLHVTLVPYIRSAGELKTKPTQHSVRELRAIGIQPDILLCRSERELSLE
                     ARRKIALQCSVREDAVIPAYDAETIYEVPIRYHTEGLDAGVLRALGLEPGVAPDLGPW
                     HEIVSRVTRPEGEVKVAVVGKYTGLMDAYKSLNEALIHGGIANNVRVVLDWIDAEHFV
                     AVDQTDRLQGVNAILVPGGFGERGAEGKIAAVTYAREQGLPFFGICFGMQLACIEAAR
                     NLAGMTGASSTEFGPCADPIVGLMTEWQRAGDVERRRADDDLGGTMRLGAYPCDLASG
                     SLVRRIYGVDRIDERHRHRYEVNIAYKERLEAAGLWFSGMSPDGRLPEIVELPGHPWF
                     VGVQFHPELRSQPMRPHPLFADFIRAARDQSILV"
     gene            9979..10818
                     /gene="kdsA"
                     /locus_tag="P5558_13970"
     CDS             9979..10818
                     /gene="kdsA"
                     /locus_tag="P5558_13970"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRY25467.1"
                     /translation="MQDVEVGRVVIGQARPLALIAGPCAIESREHALMMAERLVEITD
                     RLGLPFVFKSSFDKANRTSAASARGVGLAEGIAILGEVKSRFGCPVLTDIHDAGQCAP
                     VAEVVDILQIPAFLCRQTDILEAAARTGAVVNVKKGQFLAPWDMKNVTAKLEQFGAKR
                     ILVTERGASFGYNTLVSDMRALPELAATGWPVVFDATHSVQQPGGQGGTSGGQRQFVP
                     VLARAAVAIGVAAVFIETHDRPEKALSDGPNMVPLDKLAALLEDLMAFDGLAKSRPTL
                     VLG"
     gene            10827..12104
                     /gene="eno"
                     /locus_tag="P5558_13975"
     CDS             10827..12104
                     /gene="eno"
                     /locus_tag="P5558_13975"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRY25468.1"
                     /translation="MQDTEIVSIRGRQIMDSRGNPTVEVDVGLDCGAMGRAAVPSGAS
                     TGSREALELRDGDKSRYLGKEVLRAVDGINGEITDAILGMDATDQIAVDRAMLELDGT
                     DNKGRLGANAILGVSLAVAKAAAAASGLPLYRYLGGVGARVLPVPMMNVINGGAHADN
                     PIDIQEFMVMPVGAPSFGEALRMGAEIFHSLRKALKDAGHNTNVGDEGGFAPDLPSAE
                     AALGFLEQAVEAAGYRTGDDVVFALDCAASEFFADGRYDMAGEGRKVDSAGMVDFIEG
                     LCARFPIRSVEDGLAEGDWAGWKLLTERLGSRVQLVGDDLFVTNAAILAEGIEQGVAN
                     SILVKVNQIGSLTETLDCVRLAMASRYRAVMSHRSGETEDATIADLAVATNCGQIKTG
                     SLSRSDRLAKYNQLLRIEEELGGSAIYAGNRAF"
     gene            12122..12460
                     /locus_tag="P5558_13980"
     CDS             12122..12460
                     /locus_tag="P5558_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRY25469.1"
                     /translation="MRVDGLAYRSYRLRKQAVRHWLGVALVLVLAYFAFHSIHGSRGL
                     FAWIDRQHELETRRLELADIRAERQWLEQRVEALGPDRLATDLLEETLLGLGFVNENE
                     VVIFDEPPPQ"
     gene            12598..13635
                     /gene="pdhA"
                     /locus_tag="P5558_13985"
     CDS             12598..13635
                     /gene="pdhA"
                     /locus_tag="P5558_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRY25470.1"
                     /translation="MNATRSSAKPAARRSSRKSPADNGQWLEFYREMLLIRRFEEKAG
                     QLYGMGLIGGFCHLYIGQEAVAVGIQHALSDKDSVITGYRDHGHMLVCGMDPRNVMAE
                     LTGRATGYSKGKGGSMHMFAKDKRFYGGHGIVAGQVPLGTGIAFAHKYREDGGLCATY
                     MGDGATNQGQVYESFNMAALWKLPVLYVIENNRYGMGTSQARSSAGQKLCERGAAYGI
                     PGEQVDGMDVVAMHEKAKEVVAHIRGGEGPYILEAMTYRYRGHSMSDPAKYRKKEEVQ
                     EFREHHDPIETLRQRIVEGKLAAEDDLKAMDKEVRDIVNEAARFAQDSPEPDPSELWT
                     DILLEAEVATV"
     gene            13707..>13869
                     /locus_tag="P5558_13990"
     CDS             13707..>13869
                     /locus_tag="P5558_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25471.1"
                     /translation="MPTEILMPALSPTMTEGNLARWLKKEGDSIAPGDVIAEIETDKA
                     TMEVEAVDEG"
BASE COUNT         2051 a         4759 c         4989 g         2070 t
ORIGIN      
        1 gcaatggcgc cagggtgctg ggcgtgcgca aggccgacct gccggagggt gcgtcgctgg
       61 ccgcgatcct gcgctacccg ctctagcgcg gcggcatcgc cgtgccccgg ctcgaccgtg
      121 cctacaaggt ggcggagatg cgccggcagg cgcggcgcgt gctgccgcgc ccggtcttcg
      181 acttcgccga tggcggggcg gaggacgagc ggacgctcgg gcgcaacgag gctgccttcg
      241 aggacgtctc gctgctgccg cggccgctcg agggggcggc ggagcgggat ctctcgatcg
      301 agctcttcgg ccgccgcctc tcgatgcccc tgatgatcgg gccgaccggc ctcgccgggc
      361 tcttctggcc cggcggcgag ctctgcgccg cgcgggccgc gaccgcggcc ggcacggcct
      421 actgcctcag ccacggctcg gtctgcaccc tggaagcgct ggccgagacc ggtgcggcgc
      481 cacgctggat gcaggtcttc gtctatcgcg accggggctt cacccgcgag ctgacggatc
      541 gcgcggccgc cgccggctat gacgcgctgg tgctgaccat cgacaaccag ctcctcggca
      601 agcgcgagcg cgacatcgtc aacggcttcg gcatcccgcc gcgcttcgcc ccgctcgatc
      661 tcctgggcat ggcgacgcgg ctgccctggc tctggcggat gcgcgacgtg ctgaggacgc
      721 tcaccttcgg caactatgtc cgcgccggcc ggccctccga catccgcacg ctcgccggca
      781 gcatggccgg cctgctcgac ccggcgatga gctggcggga cgtcgaggcg ctgcgtcagc
      841 actggcaggg ccccctggtg ctcaaggggg tgctgcaccc ggacgaggcg cggcgggcca
      901 tcgagcacgg catcgacggg atcatcgtct cgaaccacgg cggccgccag ctcgacgggg
      961 cggccgcctc gctcgatgcc ctgccgcgga tcgtcgaggc cgtcgagggg cgcatggccg
     1021 tcctgctcga cggcggcgtg cgccgcggca gccacgtgct caaggccctg gcgctgggcg
     1081 ccacctgctg cctgatcggc cgcccgcagc tctggggcct ggcggtcggc ggcgaggcgg
     1141 gcgtggccag ggtcctcgag ctgctgcgcc aggagctcga ccgcgccatg gggctctgcg
     1201 gcgtcgggcg catcgccgac atcggcccgg agctcctggc cggccccggc aacgcgatca
     1261 ttccaaagga cagaagaaca tgatcgacga agtcccgctg ctgaccatcc gccgccgctt
     1321 cagccggccg agcgccgacc aggtggcggc cttccagggg ctgccgacag gcttcgtcgc
     1381 cgatgcgctg ctggcggcac ggggcggtgg caagagcgcc ctcgacgggc gggtcaagcc
     1441 gattgccgaa agcggcccgt tctgcggcgt cgtggtgccc tgcgaggccg gcccggctga
     1501 caatctagcc gtcttcggcg ctctcgatat cgcccagccg ggcgacgtgg tcatcgccgg
     1561 caccgacttc ttcaccgagg ccgcgatcat cggcgatctc ctgctcggca tgctgcagaa
     1621 caagggtgtg gtggctttcg tcacggacgg cttcgtgcgc gacatcgtcg gccttcgggc
     1681 cgtcggcctg ccctgctatg cgtcgggtgt cacgccgaac tcgccggcga agaacgggcc
     1741 gggcacggtc ggcctgcccg tccaggtggg cggcgtcctc gtcgggccgg gcgacatcgc
     1801 ggtcggcgac gaggacggcg tggtggtggt gccgcacgcc atgatcgccg ccaccatcga
     1861 caagctgccg gcgatcaagg cggccgaggc gaccatggac gcgcgggtca agggcgggct
     1921 gaagcagccc gatttctggg cggcgctgag cggctccggg cggtttcgcg aggtcgactg
     1981 agccccgggc gatggcgtct catccggtcg tgccctggaa tggtgacagg atcgtattcc
     2041 tagcacaggc agcgcgcgaa ggcaagcccg tctccggttc gctcggcatt tctcgatttc
     2101 ctggccgacc agccgccgag agaagctgca cgaggagcga aaaagccggc tgcgacaaag
     2161 gcttggctgc cgccgggttc gttggcgaga acgcgggttc gttcgtcgat tcctcgggtt
     2221 cggtcgtcgt tttgccggga tcgttcgtcg aatcgccggg ttcgctcgtc ggcgcctgtt
     2281 gcaggaatcg cgacatttcg gctcgaatgt cgcatcagac gcgccataac gattgcttat
     2341 ggcgcgtctg atgcgacatc ggcggcgcga atgttgcaga atatgcgcca acccgagccg
     2401 aaatgttgca tcggacgcac caacccgagc gctcgcgcgc gcttttcggg ccggcgaggc
     2461 ggcttggggg cgatcgcgtc gagggtgcca taagcgttcg tgatggggcc cttggacggg
     2521 acctggggag ctgggggtgt ctgcaacgac cgacagctcg atccagaagg ggatgcggca
     2581 tggctgagct caccggcggc tgcctttgcg gcaaggttcg aatcgtggcg tcggggcggc
     2641 cctatcgggt cggcctttgc cactgcctcg attgccgcaa gcatcatggc gcccttttcc
     2701 aggcaaccgc gatcttcccc gaggaggcgg tgacgatcga gggcgagacc cgcgactatg
     2761 ccgggcggtg cttctgcccc cgctgcggct cgtcggtctt cgcccgcacc ggcgacgaga
     2821 tcgaggtgag cctggggtgc ctcgattccc ccgatcagct gatgccgacc tacgagagct
     2881 ggatcgtccg ccgcgaggcc tggctgccgc cttttccgct caggcgaagc tacgtacgtg
     2941 atcgcgaggc caccggtcgc ttcgagacgt aggtttcaag cccggtcgcg gcggcgcagc
     3001 acggccagga tcagggcgca catggcgagg agctggaagc cggcgccggc taggtgggag
     3061 tattcccact gcgcccgcag cgcctcccag gtctcgggct gcaccgtcca gctctcggtc
     3121 gcccggttgg ccgggaaggt ccagacccag aagacgacct gcgccgcgat caggctggcc
     3181 agggcgacga gcgtcggccg ggcgacgcgg ccctgccggc ggtgaaggta gaggacccag
     3241 aggatgccga ggagctggac ggccagcacg atggccagcc ggttccagcc gagatagatg
     3301 gcctgcgccg tgaaataggc gtcgcgcgcc atgacgatct tgttcggcag cgcataggca
     3361 tgggcgaggg cgccgcccag cgccatggcc gtcgccagca gggcgacgaa gaaggcggtg
     3421 tcacggaaaa tcgagcggtc ggattctgcc atggctcgcg ccggcgtgct gttgccggag
     3481 agcttcaacc gccggcaccg cacggggttc ccggcgcgga gcggcgctac cggccgcggc
     3541 tggcgaagcg gacggcttcc tcggcggcgc ggatgagggc gcgggccttg ttgcagcttt
     3601 cctggtactc ggcctcgggg tcgctgtcgg cgacgacgcc gccgccggcc tggacgtaca
     3661 tggtgccgtc cttgacgagc gccgtgcgca gcgcgatgca ggtgtccatg ttgccgtcgg
     3721 ccgagaggta gcccacggca ccggcgtaga agacccggcg ctcgggctcg agctcctcga
     3781 tgatctgcat cgcccggacc ttgggcgcac ccgaggtggt gccggcggga aagccggcga
     3841 ggagggcgtc gatgacgtcg cagtcggggc gcaggttgcc ctcgacgttc gagacgatgt
     3901 gcatgacgtg gctgtagcgc tcgacgatca tccgctcggt gacctcgacc gagccgatct
     3961 cggcgacgcg cccgacatcg ttgcggccga ggtcgagcag catcaggtgc tcggccagct
     4021 ccttggggtc cgccaagagc tcctcggcca gggccacgtc ctcggcggcg tcgtggccgc
     4081 gccggcgcgt gccggcgagc gggcggacgg tgacccggcc gtcgcgcacg cggaccagga
     4141 tctccgggct cgagcccacg agcgtggcgc cgtcgaggtc gagcaggaag aggaagggcg
     4201 aggggttgag gcgccggagc gcccggtaga gcgccatggg cggcagggtg aacggcacgg
     4261 tgaagcgctg cgacggcacc acctggaaga tgtcgccggc gaggatgtac gctttcgccc
     4321 gctcgaccat ccggtgatag ccctcgcggg tcatgttgga gaccgcctcg ggcggcgggc
     4381 cggcatgcgc cggctgcgcc ggcccgatgc cgcgcgccat gtcggcgacc gcgtcggcca
     4441 ggcgctcgag cgccaggtcg taggcccggt ccgcgtccag cccggcctcc gggtagaccg
     4501 gcgtgacgat cgtgacgatg tccttgatgt tgtcgaagat ggcgatcagc gtcggcctga
     4561 ggaagacggc gtcgggcagg ccgatccggt cgggattggt ctccggcacc ggctcgacca
     4621 gccggatcat gtcgtaggcg agaaagccga agagcccggc ggccatcggc ggcagctcgt
     4681 cgggcacgtc gatgcgggtc tcggcgagca gcgagcgcag gctctccagt gtgggcagct
     4741 tctcgggctg gaagtccagc cggtcgatcc ggcagcggcg gttgatctcg gcctgcttgc
     4801 cgtggcagcg ccagacgagg tcgggcttgg tgccgatgat cgagtagcgg ccgcggaccg
     4861 caccaccctc gacgctctcc aaaaggaagc tgtaggggcg cccgtcggtg agcttgagca
     4921 tggccgagac gggggtctcg aggtcggcca ccagcgtcgt ccagaccacc tggctccggc
     4981 cggcctcgta ggcggagcgg aaggccgccc ggggcggcag gacctcttcg aagaaggcca
     5041 cggcgaaagc tccagcgtca gaattcgtcg gcggggaaga gcgaggcgat cgccgcctcg
     5101 ttgacctcga ccggatagcg acggcgcagc gccgcctcgt actggatcag cacgtcgttg
     5161 cggagggccg cggtgagctg ctccctgagt tcgggctggt cgccctccgg caccatgatc
     5221 gcgtcggtgc gcaggatggc gctgccgttg cgggtggcca ccggctcgcg tgcgaggttg
     5281 cccttgtcgg tggcgaagag ggcttcgacc gcctccggcg tcaggccggc ctcgctgccg
     5341 tctgccgccc gcccgatcgg gccgaaccgg cggagcgtga cctcgtcgcc gagcccggca
     5401 gcaatcgtct ccaatgtccg cccggagcgg gcgtcggcca tgatcgcctc gacgcgctcg
     5461 gtggcgaggc gctgccgttc ctcctgccgc cagagcgcgg ccacgtcgtc gcggacctcg
     5521 tcgagccggc gcgggcggct cggctcgctg gcgtcgacgc ggaacatgta gaaggtgccg
     5581 cccggggtct cctcgagcag ggacgtctcc tccagcggtg ccgcgaagat ggcggcgagg
     5641 atctcgggcg atggtgcccc gccctcgacc ggggcacccg tggcgtcgcg gccctgggcg
     5701 tcgatcgcct gaaagcgcct ggccgtggtg ccgacggcca gggccgcctc ctcgaggctc
     5761 tcgccggcgg cgatgccgtc atccagggcg ctcgccagat cggggagctg gtcgatggcg
     5821 cggtcgagcg cgaggtcccg gcggatctcg tccctggcgt cctcgaagct gcggacggtc
     5881 tcgggctcga cctcgatcag gcggaagaga tgccagccga aggcgctctg cacgggctcg
     5941 ctgacctcgt ccggcccgag cgcgaatatg gcgtcggcga actcgggcgg caggtcttcc
     6001 cgggccgtcg ggccgagcgg gccataggtc aggccccggt cggccatgct gtcggccagc
     6061 tcggcgaaga cggcaccttc ctcgagcgcc aggcgggcct cttcgatggt ctcgcggtcg
     6121 ctcgccagca actggccgac gcggcgccgc tcgggcgtgg tgtagaagtc cgccctcgcc
     6181 tcgtactcct cgcgcagcct cgcctcgtcg atctcgatct cgtcgacgat gtcgtcggcg
     6241 gtgagggtga ccagggtcac ggtccggtat tccggcgcct ggaaggcgtc gatgttctcc
     6301 tcgaggaacg cctcgagctc ggcgtcggtc ggctcggggg cggtcatggc atcgctcgcc
     6361 acctcgagca gggtgccgct gcgggtctcg ccgcgccagg cgccgaggcg ctcgaccagc
     6421 accgatggcg ccgcgatcga gcccgtgagg gcatccagga cgcgcgcccg gccgatatcg
     6481 gcgcgcacct gctcgacata ctgggcctcg cccaggccga gcgagcggag gtacatgtcg
     6541 aagcgcgcgc gatcgaggtt gccgtccgtg gcgaagcgct ggtcgttgcg gatggcggcc
     6601 gccacctcgg cgtcggagac gccgatgccg agatcccgcc ggtgctggtc gacgagcgct
     6661 ctggcgatga cgttctgcag gctgcggttg aggatgccga gcgatgccgc ctggcgccgg
     6721 tcgagcgtgg cgccgaactg ctcgccgaag gcgcggcggt cgatctcgaa ggcgcggtcg
     6781 acctcctgga gcggcacctc gatgtcgccg acctgcacgg cgatggcgat gtcctgccgg
     6841 ccgaagaacg tgtcgccgat gccccagacg gcgaagctgg caatgaggac caggaggagc
     6901 gcgcggacga accagctgtt ggcgcgcttg cgcatcgcat cgagcatgac ggaaggaacc
     6961 cttaaaagac gcgcccggac actattgatt gacgacgccg gcggcaatcc tcaaggcgcg
     7021 gcagcgtggc ttcgggctgc ctgccggcac cttggacagg cccgaatcgc tgtgctagaa
     7081 gccgcgcgtt cccgcaatcg gtgagagggc tggatgcctt tggcacgtcc ggtggtgctc
     7141 ggcaattgga agatgaacgg gctgcgggcc gacggtctgg cattggccgg gcggctcgac
     7201 gcgcgtgccg cgcatgcgag cggcacgctc ggcatcttcc cgccggcgac gctcgtcgcg
     7261 gcggtggccc agaggctcgc ccggagccgc gtcctcgtcg gcgggcagga ctgccacccg
     7321 gccgagagcg gcgccttcac cggctcgatc agcgccgcca tgctcgccga tgccggcgcg
     7381 cgcgcggtca tcgtcggcca ctccgagcgt cggcacgggc tcggcgagac cgatgccgag
     7441 gtcaaggcca agggcgaggc ggcgctcgcc gccgggctca tggtcgtgct ctgcatcggc
     7501 gagaccgaag ccgagtacgt ggccggggag cggcatgcgg tgctggtgcg ccagctcgac
     7561 ggcagcctgc cgagtggcgc gacggccgag aggctggtcg tcgcctacga gcccgtctgg
     7621 gcgatcggca ccggccgcac gccgagcctc gcggagatcg cccggaccca cgccgagctg
     7681 cgcggcctgc tcgccgggcg gctggccgac ggggccgagg tggcgctcct ctatggcggc
     7741 tcggtcaagc cggacaatgc cgccgacatc atggccatcg aggcggtcga cggcgccctc
     7801 gtcggcggcg ccagcctcga cgcggattcg ttctggagca tctatgcggc cggcggcggg
     7861 acctgatccc gagccgcagc gcgaaagacc tgaaaaaatg accacgatcc ttctcgtcat
     7921 ccatctcctg atcgccctcg ccctcgtcgg cgtcgtcctg atccagcgca gcgagggcgg
     7981 ggcgctcggc gggctcggcg gcaccgccat gggtggcgtc atgagcggcc gcggcaccgc
     8041 caacctgctg acccgggcga cggcgatcct cgccacgggc ttcatggtga tgagcctcct
     8101 gctcgcctat gtcgccagcg gcggcagcgg ccccgtctcg atcctcgacg ggccgaaccc
     8161 gttcgagccg gcaccggtca ccgagcccgc ggagccggca ccgccggccg ccccggcggc
     8221 gccggtcgcc aactgaggcc ttgccgggaa gacgattcgc cgggccgccg actcgccacg
     8281 gcgcggcctt tgcgcttgcc cttgtgaagc gttgcatgga tagtttcgat tgatgcctcg
     8341 cttcgtcttt atcaccggtg gtgttgtttc ctcgctcggc aagggccttg ccgcggctgc
     8401 cttgggctcg ctcctccagt tgcgcggcta tcgcgtccgg ctgcgcaagc tcgaccccta
     8461 tctcaacgtc gacccgggca cgatgtcgcc ctaccagcat ggcgaggtct tcgtcaccga
     8521 cgacggggcg gagaccgatc tcgacctcgg tcactacgag cgcttcaccg gcgtgccggc
     8581 ccgcgcctcg gacagcgtct cctccgggcg catctactgg accgtgctga cccgcgagcg
     8641 gcacggcgac tatctcggcg gcacggtgca ggtgatcccg cacgtcacca acaccatcaa
     8701 ggaatacatc cagaaggacg tcgaggacgc ggacatcgtg ctctgcgaga tcggcggcac
     8761 ggtcggcgac atcgagggcc tgccgttcct cgaggccatc cgccagctcg ccaacgagct
     8821 gccgcgcggc gaggtcgcct tcctgcacgt cacgctcgtc ccctacatcc gctcggccgg
     8881 cgagctgaag accaagccga cccagcactc ggtgcgcgag ctgcgcgcca tcggcatcca
     8941 gccggacatc ctgctctgcc gctcggagcg cgagctctcg ctcgaggccc ggcgcaagat
     9001 cgcgctccag tgcagcgtgc gcgaggacgc ggtgatcccg gcctatgacg ccgagaccat
     9061 ctacgaggtg ccgatccgct accacaccga ggggctcgat gccggtgttc tcagagcgct
     9121 cgggctcgag ccgggcgtag cacccgacct cggcccgtgg cacgagatcg tcagtcgggt
     9181 gacgcggccc gagggcgagg tcaaggtcgc ggtggtcggc aagtacaccg gcctcatgga
     9241 cgcctacaag agcctcaacg aggcgctgat ccacggcggc atcgccaaca atgtcagggt
     9301 cgtgctcgac tggatcgacg ccgagcactt cgtcgccgtg gaccagaccg accggctgca
     9361 gggggtgaac gccatcctcg tgcccggcgg cttcggcgag cggggggcgg agggcaagat
     9421 cgccgccgtc acctatgcgc gcgagcaggg cctgccgttc ttcggcatct gcttcggcat
     9481 gcagctcgcc tgcatcgagg cggcgcgcaa cctcgccggg atgactggtg cgagctcgac
     9541 cgagttcggc ccgtgcgccg acccgatcgt cggcctgatg accgagtggc agcgggcggg
     9601 cgacgtcgag cggcgccggg ccgacgacga tctcggcggc accatgcggc tcggcgccta
     9661 tccctgcgac ctggcgtccg gcagcctggt ccgccgcatc tacggcgtcg accgcatcga
     9721 cgagcgccac cggcaccgct acgaggtgaa catcgcctac aaggagcggc tggaggccgc
     9781 gggcctgtgg ttttccggca tgtcgcccga cgggcgcctg ccggagatcg tcgagctgcc
     9841 cggccatccc tggttcgttg gcgtccagtt ccacccggag ctgcgctcgc agccgatgcg
     9901 cccgcacccg ctcttcgccg atttcatccg cgccgcgcgc gaccagtcga tcctggtctg
     9961 aaccaagagg ggtaagccat gcaggacgtc gaggtcggtc gggtcgtcat cgggcaagcg
    10021 cgaccgctcg ccctgatcgc cggcccgtgc gcgatcgaga gccgcgagca cgcgctgatg
    10081 atggccgagc ggctggtcga gatcacggac cggctcggcc tgcccttcgt cttcaagagc
    10141 tcgttcgaca aggccaaccg gacgagcgcg gcgagcgcgc gcggtgtcgg cctcgccgaa
    10201 ggcatcgcca tcctcggcga ggtgaagagc cgcttcggct gcccggtgct caccgacatc
    10261 catgacgccg gccagtgcgc gccggtcgcc gaggtggtcg acatcctgca gatcccggcc
    10321 ttcctctgcc ggcagaccga catcctcgag gccgccgccc ggacgggggc cgtggtcaac
    10381 gtcaagaagg gccagttcct cgccccctgg gacatgaaga acgtcaccgc caagctcgag
    10441 cagttcgggg cgaagcgcat cctcgtgacc gagcgggggg cctccttcgg ctacaacacg
    10501 ctggtctccg acatgcgcgc cttgccggag ctcgccgcca ccggctggcc cgtggtcttc
    10561 gatgcgacgc actcggtgca gcagccgggc gggcagggcg ggaccagtgg cgggcagcgc
    10621 cagttcgtgc cggtgctggc gcgggcggcc gtggcgatcg gcgtggcggc tgtgttcatc
    10681 gagacgcacg accgcccgga aaaggcgctc agcgacggcc ccaacatggt gccgctcgac
    10741 aagctcgcgg ccttgctcga agacctgatg gccttcgacg gcctggccaa gtcgcgaccc
    10801 accctcgtgc tcggatgaga cggatcatgc aggacaccga gatcgtcagc attcgcggtc
    10861 ggcagatcat ggacagccgc ggcaacccga ccgtcgaggt cgatgtcggc ctcgattgcg
    10921 gggccatggg ccgggcagcg gtgccgtccg gcgcctcgac cggctcgagg gaggcgctcg
    10981 agctgcgcga cggcgacaag agccgctacc tcggcaagga ggtcctgcgt gccgtcgacg
    11041 ggatcaacgg cgagatcacc gacgccatcc tcggcatgga cgccaccgac cagatcgccg
    11101 tcgaccgggc gatgctcgag ctcgacggga ccgacaacaa gggcaggctg ggggcgaacg
    11161 ccatcctcgg cgtcagcctc gcggtcgcca aggcggcggc ggcggcctcg ggcctgccgc
    11221 tctatcgcta tctcggcggc gtcggcgccc gggtgctccc ggtgccgatg atgaacgtca
    11281 tcaatggcgg cgcccatgcc gacaacccga tcgacatcca ggaattcatg gtcatgccgg
    11341 tcggtgcccc cagcttcggc gaggcgctgc gcatgggcgc cgagatcttc cacagcctga
    11401 ggaaggcgct caaggacgcc ggccacaaca ccaatgtcgg cgacgagggc ggcttcgcgc
    11461 cggacctgcc gagcgcggag gcagcgctcg gcttcctcga gcaggcggtg gaagctgccg
    11521 gctaccggac gggcgacgac gtcgtcttcg ccctcgactg cgcggccagc gagttcttcg
    11581 ccgacggccg ttacgacatg gcgggcgagg ggcggaaggt cgattccgcc gggatggtcg
    11641 acttcatcga ggggctctgc gcgaggttcc cgatccgctc ggtcgaggac ggcctcgccg
    11701 aaggcgactg ggccggctgg aagctgctca ccgagcggct gggctcgcgc gtgcagctcg
    11761 tcggcgacga cctcttcgtc accaatgccg cgatcctggc cgagggcatc gagcagggcg
    11821 tcgccaacag catcctcgtc aaggtcaacc agatcggctc gttgacggag accctcgact
    11881 gcgtgcggct cgccatggcc agccgctatc gcgcggtgat gtcgcaccgc tcgggcgaga
    11941 ccgaggatgc gaccatcgcc gacctcgccg tcgccaccaa ttgcggccag atcaagaccg
    12001 gctcgctctc ccgctccgac cggctggcga agtacaacca gctcctgcgc atcgaggagg
    12061 agctgggcgg ctcggcgatc tacgccggca atcgggcctt ttgaggcagg ggggcagcac
    12121 cttgagggtc gacggtctcg cctatcggag ctaccgcctg cgcaagcagg cggtgcggca
    12181 ctggctcggc gtggccctcg ttctcgtgct cgcctatttc gcctttcact cgatccacgg
    12241 cagccgcggc ctcttcgcct ggatcgatcg ccagcacgag ctggagacca ggcgcctcga
    12301 gctcgccgac atccgcgccg agcggcaatg gctcgagcag cgcgtcgagg cgctcggccc
    12361 cgaccggctg gccacggacc tgctcgagga gacgctgctc ggtctcggct tcgtcaacga
    12421 gaacgaggtc gtgattttcg acgagccgcc cccgcaatag cggctgcctg gggcgaccat
    12481 caaattcgtt cctgcatggg ttgcatgcat ggcgaccggc tggcgcaacc tcggcgcttc
    12541 gctataagtt cacagcacag gctgcaggaa caacgcaatc gggagggccg tcgatccatg
    12601 aacgcgacca ggagcagcgc caagccggcg gcccgccgga gcagcaggaa atcccccgcc
    12661 gacaacgggc agtggctcga gttctaccgc gagatgctcc tcattcggcg cttcgaggag
    12721 aaggccggcc agctctacgg catgggtctg atcggcggct tctgccacct ctatatcggc
    12781 caggaggcgg tcgcggtcgg catccagcac gcgctctccg acaaggattc cgtcatcacc
    12841 ggctatcgcg accacgggca catgctggtc tgcggcatgg acccgcgcaa cgtcatggcc
    12901 gagctcaccg gccgggccac cggctactcg aagggcaagg gcggctcgat gcacatgttc
    12961 gccaaggaca agcgcttcta tggcgggcac gggatcgtgg cgggccaggt gccgctcggc
    13021 acgggcatcg ccttcgccca caagtatcgc gaggacggag gtctctgcgc cacctacatg
    13081 ggcgatgggg cgaccaacca ggggcaggtc tacgagagct tcaacatggc ggccctgtgg
    13141 aagctgccgg tgctctacgt gatcgagaac aatcgctacg gcatgggcac ctcgcaggcc
    13201 cgctcgtcgg ccgggcagaa gctctgcgag cgcggcgccg cctacggcat tccgggcgag
    13261 caggtcgacg gcatggatgt cgtggccatg cacgagaagg ccaaggaggt ggtcgcccac
    13321 atccgcggcg gcgagggccc ctacatcctg gaggccatga cctaccgcta tcgcggccat
    13381 tcgatgtcgg acccggcgaa gtaccgcaag aaggaggagg tccaggagtt ccgcgagcac
    13441 cacgacccga tcgagacgct gcgtcagcgg atcgtcgagg gcaagctggc ggccgaggac
    13501 gatctgaagg ccatggacaa ggaggtccgc gacatcgtca acgaggcggc ccgcttcgcc
    13561 caggacagcc ccgagcccga tccctcggag ctctggacgg acatcctgct cgaggccgag
    13621 gtcgccaccg tctgagccgt gccacccctt gccctgcgcc cgcctctggc ggcgatccat
    13681 cgaatgatcc gggaaagctg aaatcaatgc ccacggaaat cctgatgccg gcgctctcgc
    13741 cgaccatgac cgaaggcaac cttgcccgct ggctgaagaa ggagggtgac agcatcgccc
    13801 caggcgacgt catcgccgag atcgagaccg acaaggcgac gatggaggtc gaggcggtcg
    13861 acgagggca
//
LOCUS       DAOTFI010000130        13574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5420_length_13574_cov_7.161920, whole genome shotgun sequence.
ACCESSION   DAOTFI010000130 DAOTFI010000000
VERSION     DAOTFI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5420_length_13574_cov_7.161920"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="P5558_13995"
     CDS             <1..755
                     /locus_tag="P5558_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY25472.1"
                     /translation="QGYIGQALGIADVLAAAYFHALDYRADDPKWEGRDRFLLSVGHY
                     AIALYAALMEAGILPEEELATYGMDDSRLPMSGMASYTPGMEITGGSLGQGLGIAIGM
                     ALGLRRKRSKAFVYNLMSDGELGEGSTWEAAMVAAHHRLGNLICLVDFNNQQADGKST
                     QMLSAEPVEAKWRAFGWHCQRVDGNDLDAVVGAFDTARAVAEPVPRVIIFDTRMCKGV
                     PFLERREIAHFVRVEADEWVKALSALEEGRPA"
     gene            752..1795
                     /locus_tag="P5558_14000"
     CDS             752..1795
                     /locus_tag="P5558_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRY25473.1"
                     /translation="MTEASRARKHEPRKAPVTAGGKGPTTSAMIASLTAEGYETIHAP
                     FGHALVELARRDERIVGLTADLGKYTDLHVFAEAMPGRFYQMGMAEAVLMSAAAGLAR
                     EGFMPFATTYAVFAARRAYDFIAMAIAEENLPVRIVCALPGLTTGYGPSHQATEDLAI
                     FRGLPNLTIVDPCDAVDIAQMVPAIAAHDGPVYCRLLRGRVPVVLTRHKPDYRFELGR
                     AQMIRDGRDVLVIATGLMTMRALDAAVKLAQDGIGVAVLHVPTIKPLDRATILEEAGK
                     CGRLVVTAENHTVVGGLGEAVATTLLTAGVTPAFRMIGLPDAFLEAGALPTLHDMYGI
                     STDRVAAQIRAWL"
     gene            1810..2565
                     /locus_tag="P5558_14005"
     CDS             1810..2565
                     /locus_tag="P5558_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY25474.1"
                     /translation="MQGLLHGRTAIVTGAASPRGLGKATAMLFAEHGASVAILDLAAE
                     AAEGAAAGLGARHVGLACDVTDKARCEVVVAALIERWGRIDILVNNAGITQPLKIMEI
                     GPADYDAVLDVNLRGTLQMSQTVIPGMRGRRAGSIVNISSVSAQRGGGIFGGPHYAAA
                     KAGILGLTKAMARELAPAGVRVNAICPGFIATDITAGKLTPEMHDAIIAAIPMGRAGE
                     ARDVAGCALFLASDLAAYCTGTEIDVNGGSLIH"
     gene            complement(2587..3915)
                     /locus_tag="P5558_14010"
     CDS             complement(2587..3915)
                     /locus_tag="P5558_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY25475.1"
                     /translation="MAELTPAPGPARDLYDLLTGDDEARVCLDIPDAACREQPISFLL
                     HLASLSLTKIGDGLADTKLVLSWLLSVLGAPSYIVGLLVPVRESLSLLPQLMVAAWIR
                     GHAVRKWFWVAGSLIEALALGLMALAALLLEGAPAGWAILGLLALFSIGRGFASIAHN
                     DVLGKTISRTRRGTVSGWATTVGSIGTGAFGLYMVLAGGTRGTAFFAAVLALAALLWL
                     AASLLYSRIEEVPGATEGGKSAVAFIRGEVARLGQEPDFVRFVLARALFVSIALMPPF
                     LVVLSQRSEGAGIRDLGLLVLASGIAGGLSSALWGCLADRSSRQVMRAAAALAGLVAL
                     ATGSLALAAPEALALPLLLPAAVFLVALAHAGMRIGRKTYIVDLAGAGRRAGYVALSN
                     TLIGVVLVTSGLIGVFAETAGIGATLLLLAALAAVGIVLAGGLPEVQHPD"
     gene            complement(3919..5085)
                     /locus_tag="P5558_14015"
     CDS             complement(3919..5085)
                     /locus_tag="P5558_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25476.1"
                     /translation="MPFKRGPWGPRPATPAADTPESQPAAGREAQLACEQCGAVLAYA
                     PGTDSLVCAYCGHENRIEVAPTLIIEQPLEEALARGELGDDVEETQTVKCASCAAEFT
                     FDPDRHAGACPFCGHSIVTDTGVHRQLKPAALLPFLITEREAQERVRQWLRGLWFAPT
                     KLRKYARDQGRLAGVYLPYWTFDSFTRSRYEGRRGDFYTVPMQVRVNVNGRMVTQTRM
                     VQKVRWRPAAGEVSRYFDDVLVLASRSLPSWMTDRLEPWDLSDLKPYTPAFIAGFQAE
                     AYQTGLAEGWTVAAEKMQARIAGDVRLAIGGDMQEIHRLDVQHSDRTYKHILLPCWLG
                     AYLFRGKSYHLSVNARTGIVQGERPYSWIKIAAAVLLGLVVLGIALAFGNVQGF"
     gene            complement(5096..6199)
                     /locus_tag="P5558_14020"
     CDS             complement(5096..6199)
                     /locus_tag="P5558_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY25477.1"
                     /translation="MIMEKLFGEFVDVIQWTDDSPDTLAWRFERYNNEIKFGAKLTVR
                     EAQTAVFVNEGEIADVFTPGMYELRTANLPILSTLQAWPHGFESPFKAEVYFFNMRRF
                     TDLKWGTRNPAMLRDPEFGPVRLRAFGTYEIRIADPAVLLREVVGTDGHFTLDEITDQ
                     LRNLIVARFATILGSSGIPVLDLAANYDQLGQFVHGRIAPEFANYGLELTKLLVENVS
                     LPPEVEQAMDRRTSMGVIGDLRKYTQFQAAEAMRAAASNPADGGAATGIGLGMGMAMA
                     KQMADAMGAPAPAASPATPPPLPAGRSFHVARDGQPDGPFELAELRQQLRDGQLAAES
                     LVWAEGMAGWEKAASVSELAPLFRAVPPPLPGT"
     gene            complement(6221..7417)
                     /locus_tag="P5558_14025"
     CDS             complement(6221..7417)
                     /locus_tag="P5558_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HRY25478.1"
                     /translation="MTETPERVSTATQTVPADRQAALATSMAALSGELVQDLQLNLEG
                     TAVQQVEQAMRELDVTDSNSIIFFGSKAQQQLTEVSDSMLESVRTKDVGPAGDALNSM
                     VAKLRELKFEAIDPRDKPGFLARLFGAKNKLMQYLDEYETVRDQIDGITNDLERHKTT
                     LLTDITKLDKLYDVNLDYFRSLEVYIAAGRAKLKELDETVIPAMAKEVENSDDVIEAQ
                     KLRDLRARRDDLERRVHDLLLTRQVTMQSLPSIRLVQENDKSLITKINSTLANTVPLW
                     RQQLAQAITIYRSGQAAGAVKAATDLTNELLEKNADNLREVNAEVRQQVERGVFDIEV
                     VKRANDQLIATIEDSLRIADEGKAKRREAEQQLVECEAKLKESLSAASARAAGKAPPA
                     PPSRSA"
     gene            complement(7459..8349)
                     /locus_tag="P5558_14030"
     CDS             complement(7459..8349)
                     /locus_tag="P5558_14030"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HRY25479.1"
                     /translation="MSEPTSEFARRIGSRVAEPGPGGSTAMWSVSHPIALHIATFFAV
                     GPLVTGLMLGQVGKALGGLGAIAALQAAAWLIRKGRLAEREYAARTITRAPPPRKLAG
                     TALVGLAAFSLSALGAGQPVILGLVAAALMGFAAYLTYGLDPRADKGLDAKLADRAGL
                     KTEAVLAALEEARAKVRTIEAQARGLHSRELKDRLARITRSAEAVLAQIEKDPGDLRR
                     ARRFLVTYLDGTRDVVSGYRAQQQDLADTPLADNFRHVLETVERVFEEQREILKRNES
                     LDLEVQIEVLKTQLEREGVH"
     gene            complement(8546..8947)
                     /locus_tag="P5558_14035"
     CDS             complement(8546..8947)
                     /locus_tag="P5558_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HRY25480.1"
                     /translation="MLTIDPDKVCAIIVKARVFDADLPHEGDDHGSDLAHGEVDELEE
                     HVEPEDEAHDDNTEDELRSLIDDLNDDEQVELVALAWLGRGSYGIEEWAEAQAEARRA
                     HNKRTAQYLLGMPLLADYLDEGLAAFGVSCS"
     gene            9253..10401
                     /locus_tag="P5558_14040"
     CDS             9253..10401
                     /locus_tag="P5558_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HRY25481.1"
                     /translation="MTKPQRYIIRVFLFLVLAVIVVGALYDIILRAFMQNPGLNGLIL
                     GVLLLGIVYALRRIWSLRPEVAWIEAFRTSGPGFSMASPPRLLSPVAGALGEEERRGR
                     ASLNALSMRYLLDSISARLDESRDITRYQTGLLIFLGLLGTFWGLLETITSVGRVIGD
                     LSLTGDDMLGVFDGLKAGLAAPLHGMGTAFSSSLFGLAGSLVLGFIDLQATQAQNSFY
                     NDLEEWLAGLTRHSSGGSMLGELAGGAPMPAYMEALLQQTAENVDQLRLALDRSEHGR
                     GELNELLAVLNDRLGMLGERLRHDQDGLARLLGQQQTAQAALGRGGGALLDEATREHI
                     RNTDQKLGRLIDELARGREETTRELKNEIKLVSRTIAIAAGDPQSLRS"
     gene            10486..11931
                     /locus_tag="P5558_14045"
     CDS             10486..11931
                     /locus_tag="P5558_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HRY25482.1"
                     /translation="MARGRSQRSASVDIWPGFVDALSTLLMVIIFLLAVFVLAQFFLS
                     QQLEGRDAAMTRLEETVAELDRQLGLERDTAADLRRNLTRLSTDLRAALAERDAEADR
                     TDELAAERDRLAETLALSADRELRLGETLEAMRLDRQKIADEAEAARAELEAANATIA
                     ADRATLEARLAELVQLRRDIDALAQVRAELEAEIAGQADLLAAGETARADLSAELGAV
                     RDERAALEARLADEEERTRLAQRELDEREIRLDELLRSAASLEAALDAEARDREAALA
                     EVELLNRQINALSIQLAEIDNVLTQKETEIATQSATIADLGERLNVALAAQVEELSRF
                     RSDFFGRLRQVLGERDEVRIVGDRFVFQSEVLFGSGEAEIGPEGRRQLAKLAATLSAI
                     AAEIPDELPWVLQVDGHTDRRPISTARFPSNWELSTARAISVAEFLIEQGIPPERVAA
                     RGFAEFQPLDPADTPSAYRRNRRIEIKLTTR"
     gene            12054..12458
                     /locus_tag="P5558_14050"
     CDS             12054..12458
                     /locus_tag="P5558_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25483.1"
                     /translation="MTRASGDLLAAAPGAAGQPVLGAGQPIPFDSMMAVHRGALRQYR
                     VAAALLLVIGLLVVGLGLLGMLPQVPALVVGVVTAAVSVFPYRDGVERQERIDGLQVL
                     HDEWRELEGASAEPEQERERFLGLLWKLYDKR"
     gene            12480..>13574
                     /locus_tag="P5558_14055"
     CDS             12480..>13574
                     /locus_tag="P5558_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25484.1"
                     /translation="MANRISELEAHLRQWESDKRRRTAGFTLIPIAAALGVGWYAYDR
                     IGTVEEGVDRTLTALDMPAEPGAGSIARLTRLEELGNELQTLRPLPAELAATGEERDA
                     AAAALAAAESELAATSEAVATLTVERDQLAAELAAATAAADERLAQAGAKVDALRAEQ
                     AELQRRAALVERERDALSDIASERQAALDAADDARAEAEARLQALGTEQGTVRESLVA
                     QLATAGSKMAALEQELAEARARDAVAVDERIAELERQLQLSERTAAGLQVVRDDLDQR
                     LAETEDALLATETAAREEQSAHEAALAELESRREANAAALASRGRQLAELERQLQQSE
                     RTAVELQAVRDGLKQRLAETEEALVATETTA"
BASE COUNT         2056 a         4618 c         4847 g         2053 t
ORIGIN      
        1 ggcagggcta tatcggccag gcgctgggca tcgccgacgt cctggcggca gcgtactttc
       61 acgccctcga ctaccgcgcc gatgacccga aatgggaggg gcgcgaccgg ttcctgctct
      121 cggtcggcca ctatgccata gcactctatg cggcgctgat ggaggctggg attctgcccg
      181 aggaggagct cgccacctac ggcatggacg acagccggct gcccatgtcc ggcatggcgt
      241 cctacacgcc cggcatggag atcaccggcg gctcgctcgg ccaggggctc ggcatcgcca
      301 tcggcatggc gctcggtctc cggcgcaagc gctcgaaggc gttcgtctac aacctgatgt
      361 cggatggcga gctcggcgag ggctcgacct gggaagcggc catggtcgcg gcccaccatc
      421 ggctcggcaa cctgatctgc ctcgtcgact tcaacaacca gcaagccgac ggcaagtcga
      481 cccagatgct ctcggccgag ccggtcgagg cgaagtggcg ggccttcggc tggcactgcc
      541 agcgggtcga cggcaacgac ctcgacgcgg tcgtcggcgc cttcgacacc gcccgcgcgg
      601 tcgccgagcc tgtgccgcgc gtcatcatct tcgacacgcg aatgtgcaag ggcgtgccgt
      661 tcctggagcg ccgcgagatc gcccatttcg tccgcgtcga ggccgatgaa tgggtgaagg
      721 cgctttccgc cctggaggag ggacggccgg catgaccgag gcttccaggg cgcgcaagca
      781 cgagccacga aaggcgccgg tcaccgccgg cgggaagggg ccgaccacct cggcgatgat
      841 cgcctcgctg accgccgagg gctacgaaac catccatgcc ccgttcggcc atgcgctggt
      901 ggagctcgcg aggcgcgacg agcggattgt cggcctcacg gccgatctcg gcaagtacac
      961 agacctgcac gtcttcgccg aagccatgcc cggccggttc taccagatgg gcatggccga
     1021 ggcggtcttg atgtcggccg cggccggtct ggcgcgcgag ggcttcatgc ctttcgccac
     1081 cacctatgcc gtgttcgccg cgcgccgggc ttacgacttc atcgccatgg cgatcgccga
     1141 ggaaaacctg ccggtcagga tcgtctgcgc gctgcctggg ctgaccaccg gctacgggcc
     1201 gagccaccag gcgacggagg atctggcgat cttccgcggc ctgcccaacc tgaccatcgt
     1261 cgatccctgc gacgcggtcg acatcgccca gatggtgccg gcgatcgccg cccatgacgg
     1321 gccggtctat tgccgcctgc tgcgcgggcg ggtgcccgtg gtgctgaccc gccacaagcc
     1381 cgactaccgg ttcgagctgg gccgggcgca gatgatccgg gacgggcggg acgtgctggt
     1441 gatcgccacc ggcctcatga ccatgcgcgc cctcgatgcg gccgtgaagc tggcccagga
     1501 cggcatcggt gtcgccgtcc tgcatgtccc gacgatcaag cctctggacc gggcgaccat
     1561 cctcgaggaa gccggcaagt gcggccggct cgtcgtcacg gccgagaacc atacggtggt
     1621 tggcggcctc ggcgaggcgg tggcaacgac gctgctgacg gcgggcgtca cgccggcctt
     1681 ccgcatgatc ggactgccgg acgccttcct cgaggccggc gccctgccca cgctccacga
     1741 catgtacggg atctccacgg accgggtcgc ggcgcagatc cgcgcctggc tttgaaggaa
     1801 gagacgacca tgcaaggact gctgcacggc cggaccgcca tcgtcaccgg cgccgcctcg
     1861 ccgcgtggac tcggcaaggc gacggccatg ctgttcgcgg agcacggggc cagcgtggcg
     1921 atcctcgatc tggcggccga ggccgccgag ggggctgcgg ccgggctcgg cgcgcggcat
     1981 gtcggcctcg cctgcgacgt caccgacaag gcgcgctgcg aggtggtggt ggcggcgctg
     2041 atcgaacggt ggggccggat cgacatcctg gtcaacaatg ccggcatcac ccagcccctg
     2101 aagatcatgg agatcggacc cgccgactat gacgccgtgc tcgacgtcaa cctgcgcggc
     2161 acgctgcaga tgagccagac ggtgatcccg ggcatgcgcg ggcgccgggc gggcagcatc
     2221 gtcaacatct cgtcggtctc cgcccagcgc ggcggcggca tcttcggcgg cccccattat
     2281 gcggcggcca aggcgggcat cctcgggctc accaaggcga tggcgcgcga gctggcgccg
     2341 gccggggtca gggtcaacgc catctgcccg ggcttcatcg ccaccgacat caccgccggc
     2401 aagctgacgc ccgagatgca cgacgcgatc atcgccgcca ttccgatggg ccgtgccggc
     2461 gaggccaggg acgtcgccgg ctgcgccctg ttcctcgcct ccgatctcgc cgcctactgc
     2521 accggcaccg agatcgacgt caatggcggc tccctcattc actgagccgg gggccccagt
     2581 aagcgcctag tcggggtgct gcacttccgg caggccgccg gcgaggacga tgccgaccgc
     2641 ggctagggcg gccaacagca ggagcgtggc gccgatgccg gcggtctcgg cgaagacgcc
     2701 gatgaggccg ctcgtcacga ggacgacgcc gatcagggtg ttgctgagcg ccacgtagcc
     2761 ggcccggcgg ccggcaccgg cgagatcgac gatgtaggtc ttgcgcccga tccgcatccc
     2821 ggcatgggcc agggccacga ggaaaacggc agcgggcagc agcagcggca gggcgagggc
     2881 ctcgggcgct gccaaggcga ggctgcccgt cgccagggcg acgaggccgg cgagcgctgc
     2941 cgcggcgcgc atcacctggc ggctcgagcg gtcggcgagg cagccccaaa gggccgagct
     3001 caacccgccg gcgatgcccg aggcgaggac gaggaggccg aggtcacgga tcccggcacc
     3061 ctccgagcgc tggctgagca cgacgaggaa gggtggcatc agggcaatgc tgacgaagag
     3121 cgctcgcgcc aggacgaagc gcacgaaatc gggctcctgg cccagccgtg caacctcgcc
     3181 gcggatgaag gcgaccgcgc tcttgccgcc ctcggtcgca cccggcacct cctcgatccg
     3241 gctgtagagc aggctggccg ccagccagag cagggccgcc agcgccagga cggcggcgaa
     3301 gaaggcggtg ccgcgcgtgc cgccggccag caccatgtag agcccgaagg cgcccgtgcc
     3361 gatgctgccg accgtcgtcg cccagccgct gaccgtgccg cgccgggtcc ggctgatggt
     3421 cttgccgagc acgtcgttgt gcgcgatcga ggcgaagccg cggccgatgc tgaagagggc
     3481 gagcaggccg aggatggccc agccggccgg ggcaccctcc agcagcagcg ctgccagcgc
     3541 catcagaccg agggccagag cctcgatcag gctcccggcg acccagaacc acttgcgcac
     3601 ggcatggccc cgaatccagg cggcgaccat gagctgcggc aggagcgaga gcgattcgcg
     3661 caccggcacg agcaggccga cgatgtagct cggcgcgccg agcaccgaaa gcagccagct
     3721 cagcacgagc tttgtgtcgg cgaggccgtc gccgatcttg gtcagggaca ggctggcgag
     3781 atgcaagaga aagctaatcg gctgctcgcg gcaggcggcg tcgggaatgt cgaggcagac
     3841 ccgtgcctcg tcgtcgccgg tgagtaggtc gtagagatcg cgcgccggtc ccggtgccgg
     3901 agtgagctcg gccatggctc aaaagccctg cacgttgccg aaggcgaggg cgatgccgag
     3961 cacgacgagg ccgagcagca cggcggcggc gatcttgatc cagctgtagg ggcgctcgcc
     4021 ctggacgatg ccggtccgcg cattgacgct cagatggtag ctcttgcccc ggaagagata
     4081 ggcgccgagc cagcagggca ggaggatgtg cttgtaggtg cgatcgctgt gctggacgtc
     4141 gaggcgatgg atctcctgca tgtcgccgcc gatggcgagc ctgacgtcgc cggcgatccg
     4201 cgcctgcatc ttctcggccg ccacggtcca gccctcggcg aggccggtct ggtaggcctc
     4261 ggcctggaag ccggcgatga aggcgggcgt gtagggcttg aggtcggaga ggtcccaggg
     4321 ctccagccgg tcggtcatcc agctcggcag cgagcggctg gccagcacca gcacgtcgtc
     4381 gaaataccgg ctgacctcgc ccgccgccgg tcgccagcgc accttctgga ccatccgcgt
     4441 ctgggtcacc atgcggccgt tcacgttgac ccggacctgc atcggcacgg tgtagaaatc
     4501 gccgcgccgg ccctcgtagc ggctccgggt gaagctgtcg aaggtccagt acggcaggta
     4561 gacgccggcg aggcggccct ggtcgcgggc gtatttcctg agcttcgtcg gcgcgaacca
     4621 gaggccgcgc agccactggc gcacccgctc ctgcgcctcg cgctcggtga tcaggaaggg
     4681 caggagggcc gccggcttga gctggcggtg gacgcccgtg tcggtgacga tcgaatggcc
     4741 gcagaacggg catgccccgg catggcggtc cgggtcgaag gtgaattcgg ccgcacagga
     4801 agcgcatttg acggtctgcg tctcttcgac gtcgtcgcca agctcgcccc gggcgagcgc
     4861 ttcctcgagc ggctgctcga tgatcagggt cggggccacc tcgatcctgt tctcgtgacc
     4921 gcaataggcg cagacgagcg agtcggtgcc aggtgcatag gcgagcacgg cgccgcactg
     4981 ctcgcaggcg agctgcgcct cgcgaccggc ggccggctgg gactctggcg tgtcggccgc
     5041 gggggtggcg ggccgcgggc cccaaggacc ccgcttgaac ggcacgggcg acgcttcagg
     5101 tgccgggcag cggcggcggc acggcgcgga agagcggcgc cagctcgctc acggaggctg
     5161 ccttctccca accggccatg ccttcggccc aaacgaggct ctcggccgcg agctgcccgt
     5221 cgcgcagttg ctggcgcagc tcggcgagct cgaagggacc gtcgggctgg ccgtcgcggg
     5281 caacatggaa ggaccggccg gcgggcaggg gcggcggcgt ggccggggac gccgccggcg
     5341 ccggggcacc catcgcgtcc gccatctgct tcgccatcgc catacccatg ccgagcccga
     5401 tgccggtcgc cgccccgcca tcggccgggt tggaggctgc cgcgcgcatc gcctcggccg
     5461 cctggaactg ggtgtatttg cgcaggtcgc cgatgacccc catggaggtc cgccggtcca
     5521 tcgcctgctc gacctcgggc ggcagcgaga cgttctcgac caggagcttc gtgagctcga
     5581 gcccgtaatt ggcgaactcc ggcgcgatcc ggccgtgcac gaactggccg agctggtcgt
     5641 aattggcggc gaggtcgagc accgggatgc cggagctgcc gaggatggtg gcgaagcggg
     5701 cgacgatcag gttcctgagc tggtcggtga tctcgtcgag ggtgaagtgg ccgtcggtgc
     5761 cgaccacctc gcgcagcagc acggccggat cggcgatcct gatctcgtac gtgccgaagg
     5821 cccgcagccg caccggcccg aactcggggt cacgcagcat ggccgggttg cgcgttcccc
     5881 atttgaggtc cgtgaaccgg cgcatgttga agaaatagac ctcggccttg aacgggctct
     5941 cgaagccgtg cggccaggcc tgcagggtcg agaggatcgg caggttggcg gtccgaagct
     6001 cgtacatgcc gggggtgaag acgtcggcga tctcgccctc gttgacgaag accgctgtct
     6061 gcgcctcgcg caccgtcagc ttggcgccga acttgatctc gttgttgtag cgctcgaacc
     6121 gccaggcgag cgtgtccggc gagtcgtccg tccactggat gacgtcgacg aactcgccga
     6181 agagcttttc catgatcatg ggccgatcct tctctgccgg tcaggccgac cgcgacggcg
     6241 gggcgggggg cgccttgccg gcggcccggg cggaggcggc gctcaggctc tccttcagct
     6301 tcgcctcgca ctcgacgagc tgctgctccg cctcacgccg tttcgccttg ccctcgtcgg
     6361 cgatgcgcaa gctgtcttcg atggtcgcga tgagctggtc gttggcacgc ttgaccacct
     6421 cgatgtcgaa gacgccacgc tcgacctgct ggcgcacctc ggcattgacc tcgcgcaggt
     6481 tgtcggcgtt cttctcgagc agctcgttgg tgaggtcggt ggccgccttg acggcgcccg
     6541 ccgcctggcc ggagcggtag atggtgatcg cctgggcgag ctgctggcgc cagagcggca
     6601 cggtgttggc aagcgtcgag ttgatcttgg tgatcaggga cttgtcgttc tcctgcacca
     6661 gccggatcga cggcaggctc tgcatggtca cctggcgggt gagcagcagg tcgtgcaccc
     6721 ggcgctcgag gtcgtcgcgg cgggcccgca ggtcccgcag cttctgcgcc tcgatcacgt
     6781 cgtcggaatt ctcgacctcc ttcgccatcg ccggaatgac cgtctcgtcc agctccttca
     6841 gcttggcccg gccggcggcg atatagacct cgagcgagcg gaaatagtcg agattgacgt
     6901 cgtagagctt gtcgagcttg gtgatgtcgg tgagcagcgt ggtcttgtga cgctcgagat
     6961 cgttggtgat gccgtcgatc tggtcgcgca ccgtctcgta ctcgtcgaga tactgcatca
     7021 gcttgttctt ggcgccgaag aggcgggcca ggaagcccgg cttgtcgcgg ggatcgatcg
     7081 cctcgaactt cagctcgcgc agcttggcga ccatcgagtt gagggcgtcg ccggccgggc
     7141 cgacatcttt cgtccgcacc gattcgagca tcgaatccga gacctcggtc agctgctgct
     7201 gggccttgga gccgaagaag atgatcgagt tgctgtcggt gacgtcgagc tcgcgcatcg
     7261 cctgctcgac ctgctgcacc gccgtgcctt cgagattcaa ctgcaagtcc tgaaccagct
     7321 cacccgacag ggctgccatg ctggtggcca gcgccgcctg ccggtcggcc ggcaccgtct
     7381 gggtcgcggt cgacacccgt tcgggtgttt cggtcatcgc tcgtctcccg gattggcacc
     7441 ggcccgaagg gcggtggctc agtggacacc ttcgcgttcg agctgcgtct tcagcacctc
     7501 gatctgcacc tcgagatcga ggctttcgtt gcgcttgaga atctcgcgct gctcctcgaa
     7561 cacccgctcg actgtctcca ggacatggcg gaaattgtcg gcgagcggcg tgtcggccag
     7621 atcctgctgc tgggcgcggt agccgctaac cacatcgcgg gtgccgtcga gataggtcac
     7681 gaggaagcgt cgcgcccggc gcagatcgcc cgggtccttc tcgatctggg cgagcaccgc
     7741 ctcggcactc cgggtgatcc gggcgaggcg gtccttgagc tcgcgcgaat gcagcccccg
     7801 tgcctgcgcc tcgatcgtcc gcaccttggc gcgggcctcc tcgagggcgg cgagcacggc
     7861 ctcggtcttg agcccggcac gatcggcgag cttcgcatcc aggcccttgt cggcgcgcgg
     7921 atccaggcca taggtgagat aagcggcaaa gcccatgagc gctgcggcga cgaggccgag
     7981 gatcaccggc tggccagcgc cgagcgcgct gagcgagaag gcggcgaggc cgaccagcgc
     8041 tgttccggct agcttgcgcg gcgggggcgc gcgggtgatc gtccgcgcgg catattcgcg
     8101 ctccgcgagg cggcccttgc ggatcagcca ggccgccgcc tggagtgctg cgatggcacc
     8161 gagcccgccc agcgccttgc cgacctggcc gagcatcagg cccgtgacca gggggccgac
     8221 ggcgaagaag gtggcgatgt gcagggcgat cggatggctc accgaccaca tggcggtcga
     8281 tccgcccgga cccggctcgg cgacccgcga gccgatccgt cgcgcgaatt cgctggtcgg
     8341 ctcgctcatc ggcccggtcc gtccatgtaa tcccaccgcg cgctcaaaag agcgcccgca
     8401 tggcgaggag ccagaagccg aagagcagca ggcccgtgcc gagaccgcgg gtcacggccg
     8461 tccgtcccgg cgagcgtacc gcgcgaccat gcgcggatcc gcgatcgctc gttgctcggg
     8521 cacgttcacc tggcgcttcc ttccctcagc tgcagctgac gccgaaggcc gccagccctt
     8581 cgtcgagata gtccgcgagc agcggcatgc ccagcaggta ttgcgcggtg cgcttgttgt
     8641 gcgcgcggcg cgcctcggcc tgcgcctcgg cccattcctc gatgccgtag ctgccgcggc
     8701 cgagccaggc cagcgccacc agctcgacct gctcgtcgtc gttgaggtcg tcgatcagcg
     8761 aacgcagctc gtcctcggta ttgtcgtcgt gcgcctcgtc ctcgggctcg acatgctcct
     8821 cgagctcatc gacctcgcca tgcgccagat ccgagccatg atcgtcgccc tcgtgcggca
     8881 gatcggcgtc gaacacccgg gccttgacga tgatggcgca aaccttgtcg ggatcgatgg
     8941 tcagcatgct ggtcgagtct ccttgcaccg cttcttgcca tgccgaagat ggtgtggctc
     9001 atcgcgtcgg catcgatctt tcgcaaattt cggcacatgg cgcaaagcga gtcggaaccc
     9061 gctgccgcgg atcgcgccgg cggcgcagtg ccggcacgcc acaaccggct tgccggattc
     9121 accgaaaatc catgaattac tggtagagcc ggctatagac atactatcga ggcgttagcg
     9181 tccgatcctt ctggcccgca ccatgcccag accgacccag ccccgttcga tcctggcgag
     9241 cccaatccgc ccatgaccaa gccgcaacgc tacatcatcc gggtttttct gttcctcgtg
     9301 ctcgcggtga tcgtggtcgg ggcgctctac gacatcatcc tgcgcgcctt catgcagaac
     9361 ccgggattga acgggttgat cctcggcgtg ctcctgctcg gcatcgtcta cgcgctccgc
     9421 cgtatctggt ccctgcgccc cgaggtcgcc tggatcgagg ccttccgcac gtccgggccg
     9481 ggcttctcga tggccagccc accaaggctc ctctccccgg tggccggggc gctcggcgag
     9541 gaggagcgcc gcggccgggc ctcgctcaat gcgctatcca tgcgctatct gctcgacagc
     9601 atcagcgcca ggctcgacga gagccgcgac atcacccgct accagaccgg cctgctgatc
     9661 tttctcggac tgctcggaac cttctggggg ctgctcgaga cgatcacctc ggtcgggcgg
     9721 gtgatcggcg atctctcgct caccggcgac gacatgctcg gtgtcttcga cgggctcaag
     9781 gccgggctgg cggcaccgct gcacggcatg ggcacggcct tcagctcgtc gctcttcggt
     9841 ctcgccggct cgctcgtcct cggcttcatc gacctgcagg cgacccaggc gcagaacagc
     9901 ttctacaatg acctcgagga gtggctcgcc gggctgacgc gacattcctc gggcggcagc
     9961 atgctcggcg agctcgccgg cggggcgccg atgccggcct atatggaagc cctgctgcag
    10021 cagacggcag agaacgtcga ccagctgcgg ctggcgctcg accgcagcga gcatgggcgc
    10081 ggcgagctga acgagctttt ggccgtgctc aacgatcgcc tcggcatgct cggcgagcgg
    10141 ctgcggcacg atcaggacgg cctcgcccgg ctgctgggcc agcagcagac cgcacaggcg
    10201 gcgctcggtc gtggaggcgg ggcgctgctc gacgaggcga cgcgcgagca catccgcaac
    10261 accgaccaga agctcggccg gctgatcgac gagctggcgc gtggtcgcga ggagaccacc
    10321 cgcgagctga agaacgagat caagctggtc tcgcgcacca tcgccatcgc cgccggcgat
    10381 ccgcagtcgc tgcggtccta gccgacggat cgggcgatgg gcgaagagcg agacgttatg
    10441 agcggtcgcc ggctccgccg gcccggtcgg cgggtgggct gaggcatggc ccgcggccgc
    10501 agtcagcgca gtgcgtcggt cgacatctgg cccggcttcg tcgacgcgct gtcgacgctg
    10561 ctcatggtca tcattttcct cctcgcggtc ttcgtcctgg cgcagttctt cctcagccag
    10621 cagctcgagg gtcgtgacgc agcgatgacc cggctcgagg agacggtggc cgagctcgac
    10681 cggcagctcg ggctcgagcg cgacacggcg gctgatctgc ggcgcaacct gacccggctc
    10741 tcgaccgatc tcagggcggc gctcgccgag cgcgatgccg aggccgaccg taccgacgag
    10801 cttgccgccg agcgcgaccg cctggccgag accctggcgc tcagcgccga ccgagagctc
    10861 aggctgggcg aaacgctgga ggcgatgcgg ctcgaccggc agaagatcgc cgacgaggcc
    10921 gaggccgcac gggccgagct cgaggcggcg aacgccacca ttgccgccga tcgcgcgacg
    10981 ctggaggccc ggctcgccga gctcgtgcag ttgcggcggg acatcgatgc gctggcgcag
    11041 gtgcgtgccg agctcgaggc cgagatcgcg ggacaggccg atctcctggc tgccggcgag
    11101 acggcgcggg cggatctgtc ggcggagctc ggtgccgtgc gcgacgagcg ggcggcgctc
    11161 gaggcacgcc ttgcggacga ggaggaacgc acccggctgg cgcagcgcga gctggacgag
    11221 cgcgagatcc gcctggacga gctgctgcgc agcgccgcga gcctcgaggc cgcactcgac
    11281 gcggaagcgc gtgaccgcga ggcggcgctc gccgaggtgg agctgctcaa ccgccagatc
    11341 aacgccctgt cgatccagct cgccgagatc gataacgtgc tgacccagaa agagacggag
    11401 atcgcgacgc agagtgcgac catcgccgat ctcggcgagc ggttgaacgt ggcgctggca
    11461 gcgcaagtgg aggagctctc gcgtttccgc tccgacttct tcggccggct tcgccaggtt
    11521 ctgggcgagc gtgacgaggt gcgtatcgtc ggcgaccgct tcgtcttcca gtccgaggtg
    11581 ctgttcggct cgggcgaggc ggagatcggt cccgagggcc gccgccagct cgcgaagctc
    11641 gccgcgaccc tgagtgccat cgccgccgag attccggacg agctgccctg ggtcctgcag
    11701 gtcgacggcc ataccgaccg gcgaccgatc agcaccgcgc gcttcccttc caactgggag
    11761 ctgtcgaccg cccgggcgat ctccgtggca gagttcctga tcgagcaggg cattccaccc
    11821 gaacgggtcg ccgcccgggg cttcgccgag ttccagcccc tcgatccggc cgacacgccg
    11881 agcgcctatc gccgcaaccg gcgcatcgag atcaagctga cgacccgctg accggcggtc
    11941 gcggtccgct gcgattgctt ttcccgcccg atccggcaag actgcgtcag gccgggatgc
    12001 tcaaaggagt ggtcgaccac cggtgacggt ccaaaaaagg cggggagggc ttgttgacgc
    12061 gcgcatcggg tgacctcttg gccgcggcgc ccggcgccgc gggccagccg gtgctcggcg
    12121 ccggccaacc aattccgttc gacagcatga tggcggtgca tcgcggtgcc ctgcggcagt
    12181 accgggtggc ggccgcgctc ctgctcgtga tcggcctgct ggtggtcgga ctcggcctcc
    12241 tcggcatgct gccgcaggtg ccggcgctgg tcgtcggtgt ggtgacggcg gcggtctcgg
    12301 tctttcccta tcgtgacggg gtcgagcgcc aggagcggat cgacggcctg caggtgctgc
    12361 acgacgagtg gcgcgagctc gagggcgcaa gcgccgagcc ggagcaggag cgcgagcgtt
    12421 tcctcgggct cttgtggaag ctctacgaca aacgttgaag gtcttgctgc ggggtggcga
    12481 tggcgaacag gatctccgag ctcgaggcgc atctgcgcca gtgggaaagc gacaagcgcc
    12541 ggcgcacggc gggcttcacg ctgatcccga tcgcggccgc cctcggcgtt ggttggtatg
    12601 cctatgatcg gatcggcacg gtcgaggaag gcgtcgaccg gacactgacg gcgctcgaca
    12661 tgccggcaga gccaggtgcc ggctcgatag ccaggctgac ccggctggaa gagctcggca
    12721 acgagctgca gaccttgcgg ccgctgccgg ccgagctggc cgccacaggt gaggagcgtg
    12781 acgccgctgc ggcggcgctc gcagccgccg agagcgagct ggcggcgacg agcgaggccg
    12841 tggcgacgct caccgtcgag cgtgatcaac tcgcggccga actggcggcg gcgaccgctg
    12901 ccgccgacga gcgtctggcg caggccggcg ccaaagtcga cgctctgcgg gccgagcagg
    12961 ccgagctgca gcgccgggca gcactggtcg agcgcgagcg tgacgcgctc agcgacatcg
    13021 cgagcgagcg gcaggcggcg ctggacgctg ccgacgatgc gcgggccgag gccgaggcgc
    13081 ggctgcaggc gctcggcacc gagcagggca cggtccgaga atccctggtg gcgcagctcg
    13141 ccacggcggg cagcaagatg gcggccttgg agcaggagct cgccgaggcg cgggcgcgcg
    13201 atgcggttgc ggtcgatgag cggatcgccg agctggagcg gcagctgcag ctatccgagc
    13261 ggacggcggc cggcctgcag gtggtgcggg atgatctgga tcagcgtctg gccgaaaccg
    13321 aggatgcgct gcttgccacc gagaccgccg ccagggaaga gcagtcggcc cacgaggcgg
    13381 ccctcgcgga gctggagagc cggcgcgagg cgaatgcggc ggcgctcgcc tctcgcggtc
    13441 ggcaactcgc cgagctggag cggcagctgc aacagtccga gcggacggcc gtcgagctgc
    13501 aggccgtgcg cgacggtctc aagcagcgct tggccgaaac ggaggaagcg ctggtcgcca
    13561 cagagaccac cgcc
//
LOCUS       DAOTFI010000131        13537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5442_length_13537_cov_6.771621, whole genome shotgun sequence.
ACCESSION   DAOTFI010000131 DAOTFI010000000
VERSION     DAOTFI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5442_length_13537_cov_6.771621"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="P5558_14060"
     CDS             complement(<1..264)
                     /locus_tag="P5558_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25485.1"
                     /translation="MINRNHWSISGFSAMALLALSACSGGGGGSGGGTPSTPSGGGGG
                     GGGGTPPVVDFAVPNPYATAGSESPDKLGRLFVFGDSYSDPFDP"
     gene            complement(637..1440)
                     /locus_tag="P5558_14065"
     CDS             complement(637..1440)
                     /locus_tag="P5558_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY25486.1"
                     /translation="MRKSRGFTRFACGLLTLLGLAMATPPAEARGCFPIAERLLPGVR
                     LASLPDGATVGLTYLGHSSFWIETTGGTTVITDFNGHHTGPALPDIVTMNNAHSTHFT
                     TLPDPGIRHVLRGWKPEGGVAEHDLELEDLRIRNVPTSVHGRSGAQANSNSIFVFEVE
                     DLCIAHLGHLHHVLTDMHLGELGVIDILLVPVDGSYTLAQAAMKEVIEQIRPSVVIPM
                     HYFGTASLDRFLALMGEDWDVVTFAGSSLAYSRITLPQRQVAVLQPGRW"
     gene            1507..2715
                     /locus_tag="P5558_14070"
     CDS             1507..2715
                     /locus_tag="P5558_14070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY25487.1"
                     /translation="MLHRSLIRNVSILATCQAFFFMANTIQITTSALVGLQIAPSPAL
                     ATLPLAAQFVGNMLATMPASLLMARIGRRLGLLLGSLFGVLSGLLATWAVVQASYPLF
                     MTAGLLYGVFGAFSQYFRFTAADAADASDARDKVAARARAIALVMAGGLVAAVLGPEL
                     AKATQDLLPPFVFAGCYLMIATVAFCCGVALAFLDLPHKVATTSDAPARPLLEIVRKP
                     EAITAFAAALVAYVTMNMLMTATPLAMLACGYAFADSAMVIQWHVVGMFAPSFFSGSL
                     IARFGAERIIAFGGLLMLACIGTNLAGIEVVNFVASLLLLGLGWNFMFIGGTTLLTQA
                     YRPSEKAKVQGFNDLCLFSAVAASAAASGALHEWLGWQPMNIAALPLLLAVILLAVIR
                     AARRPLAAPV"
     gene            2746..4308
                     /locus_tag="P5558_14075"
     CDS             2746..4308
                     /locus_tag="P5558_14075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY25488.1"
                     /translation="MSAFADHLQLSKHAARGRHGVVAAQNLKAATVGAEVLKAGGHAI
                     DAAVATSLALAATEPWMSGLGGGSTMLILDRATGEVESIDAGMIAPAALDPAAYPLVG
                     GTAGDLFAWPAVVEDRNVLGPLSFAVPGHLAGLGLAHERQGRMTFAELVGPARQLAKD
                     GLEIDWYAALLVGTAARDLRRFPSSAEVWLADGLPPTPAVAGEPLKLPLGRLAETLDL
                     LAEEGPKTLYTGGLAELVAADAAALGSPLAAADLAAYRASVAPALRIPYGEAEVFGMP
                     GLFAGSTLRRCLEALAGWSLASDSPGRDAYLAFAEVLKGAYAKRLAEDGHASDGPAPS
                     CTSHLSVIDAEGNMVALTQTLLSLFGSRVVLPSTGMLMNNGVMWFDPRPGRPNSIAPG
                     VRPLSNMCPVIATDATRRLALGASGGRRILPAVLQLLSFILDNHMSLEEAFAEPRIDV
                     SGDELVTMSRDLDEEVRAALAEAHGATTVRAEVYPMRFACPVAVLHDLLLDDRYGTAE
                     VIHPWADAVAVQ"
     gene            4373..5863
                     /locus_tag="P5558_14080"
     CDS             4373..5863
                     /locus_tag="P5558_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25489.1"
                     /translation="MRKLAGLIGMAGLALGLHLAMVENATAAQDQLVMGMVLEPPHLD
                     PTAGAAAAIREVTYANIFEGLTRIDRSGSVQPALARSWTISDDGLTYTFELADGVTFH
                     NGVPLLAEHVVFSFERAMAEDSVNAQKGLFEPIASVEAPDDGTVVITLERPTGSFLFN
                     LGWGDMVIVEPGSAPTNKNEPVGTGPFRFVRWVKGDRVEIERNPDYWGEAPALAKATF
                     KFIPDPAAATAAMLAGDIDAFANFPAPEALVQFEADPRFVVVIGSTEGETILAINNAK
                     PPLDDIRVRRALAHAIDRQAIIDGAMMGYGTPIGSHFAPHNPAYVDLTGTYPYDPEKA
                     KALLAEAGAEDLELTLKLPPPSYARRGGEIIQAELAAVGIEAEIIPMEWAQWLEQVFT
                     GKDYDLTIVSHTEPMDIGIYARPDYYFQYQSEAFNEVMDKLAAVTDAAERQALLGEAQ
                     EILAEDSVNVFLFQLAKHGVWKAGIEGLWHDSPIPANDLTEVHWTE"
     gene            5880..6881
                     /gene="dusB"
                     /locus_tag="P5558_14085"
     CDS             5880..6881
                     /gene="dusB"
                     /locus_tag="P5558_14085"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRY25490.1"
                     /translation="MATPIQIGPLTLASPVLMAPMTGISDLPFRRLVARFGAGLIFSE
                     MIASPHLLAETAKTRRQARFDAASRPWAVQLAGTEPAVLAEAARYVVDRGADLVDLNF
                     GCPAKLVVGKAAGSALMRDEALAGRILDAVVEAVPVPVTLKMRLGWDDGNRNAARVAR
                     IAEEAGIRMLTVHGRTRSQLFKGAADWAAVAEVKAAVGLPVVVNGDITDAATASRALA
                     LSGADGVMIGRGACGRPWLLAQIAAALDGAGEPAEPTRDEMLAIVLEHHAAMLGHYGR
                     DLGLRHARKHLAWYLAALPGGAGLARRVNRLDDAEAVVAALREIYASAADTEPALAA"
     gene            6963..7673
                     /locus_tag="P5558_14090"
     CDS             6963..7673
                     /locus_tag="P5558_14090"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HRY25491.1"
                     /translation="MHIARMAAAALSILLALTTVEARGQGEAPLFSLDFADYQPADGS
                     VRDWLAGRGFDFAKAADDDRKISVSVEDGVLQVDTKKQAFGVIYHEGDVPRATRLRVA
                     WGVDVFPKDVGYEKAVNNEALMIYVFFGREKKPSGSVFIPDVSYFIGFYLCDSDRVDH
                     PYKGRYHHESGRFVCLAHPKPGETIVSEVDLRAAFESYFGRADMPTVSGISLEVDTSD
                     AGDGGKARAFLEKVELLP"
     gene            complement(7690..8328)
                     /locus_tag="P5558_14095"
     CDS             complement(7690..8328)
                     /locus_tag="P5558_14095"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HRY25492.1"
                     /translation="MRNRARIDRRGLIFMGAALPALALGACQLPGQSAPPRQFRVTPK
                     TTFDELPQVDWALAVDRPTVDRSIDTNRIARVSGVEVEFYADAAWVDRPPAMIQPLII
                     QSFRSSGAIAVVTDRRSEVRPDFMLQTDIIAFQALQVESGPPEARVVMSAKLIAMPRR
                     DVVGTTEIGRTVPAAAGDLPAIVAAFDDALGKVLKRLVEWTLVTGKAGAVAT"
     gene            complement(8383..9408)
                     /locus_tag="P5558_14100"
     CDS             complement(8383..9408)
                     /locus_tag="P5558_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRY25493.1"
                     /translation="MSSKASPSVIGAFVLGGLALLALGIVVFGGTRWFQERHPLVAYF
                     PGSIAGLRVGAPVNFRGVNVGQVTEIKVMFEPETVQARIPVILEMDRDRIVAVGEDLT
                     VDDDAFRASLFDKGFRAQLGLESFVTGLLSVNLDFFEDAPPPEFHNKGQFELPYPELP
                     TIQSDLQLLQANAGEIAQQLTASLDKMNEVFGSLGTAASENRGRLASVFDSFARMAED
                     LSAVTPKLAALVDESTGTAAAVTRTADTANGLLEDNAAGLAKTLADLQESVAALRKMA
                     DQVNNMVAENRAGMRDFTGTGLYEITGLAQDAQRMVDQITRVMEDLERDPARFLFGDR
                     AQGVRAE"
     gene            complement(9405..10205)
                     /locus_tag="P5558_14105"
     CDS             complement(9405..10205)
                     /locus_tag="P5558_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY25494.1"
                     /translation="MPSASEAPFIAVRGLTIAYGSFVLMKDIDFVIQKGDVFVIMGGS
                     GCGKTTLMRAMVGLKAPARGEVRYGDASFWATDERARANLMQRFGVLYQQGALWSSMT
                     LAENVALPLGEYTDLSAAEIRDMVSFKLALVGLAGFEDYYPSEISGGMRKRAGLARAI
                     ALDPEVLFFDEPSAGLDPISSKRLDDLILELRESLGATIVMVTHELPSIFAIANNSVF
                     LDAETRTQLATGDPKRLLAETEEPKVREFLTRGGGEGGERSPQEHAQP"
     gene            complement(10189..11346)
                     /locus_tag="P5558_14110"
     CDS             complement(10189..11346)
                     /locus_tag="P5558_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25495.1"
                     /translation="MAAAIQSNDETAGFEVVGDPTGRGSLRLVLFGDWLTRRGVPSAE
                     RVHAELKGDAVTSVRLDTARLGAWDTAFLTFLRKLERLTAADGPKLDTSSLPEGATRL
                     LGLAASVPERAGARKAAARPPLLERVGKGTQAFLEACGETLAFIGEVVIAFGRLVRGR
                     ARFRWSDFFEVVQEVGAKALPIVGLISFLVGVILAFLGAIQLLQFGAQIFVADLVAIG
                     MARDMAAMMVGIILAGRTGAAFAAQLGTMQVNEEIDALSTLGLSPMEFLVLPRMLALI
                     LMTPLLCIYANLLGILGGLFVGVVMLDLPAALYLQQTQASTPLLFFAGGLLKATVYGV
                     VVAVCGCLRGMQCGRSAAAVGNATTSAVVTSIVFIILSMAALTVIYDALGI"
     gene            complement(11482..12228)
                     /locus_tag="P5558_14115"
     CDS             complement(11482..12228)
                     /locus_tag="P5558_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY25496.1"
                     /translation="MGKLDGRTALVTGGCSGIGRAIADLFAREGARVGIVDLDAARAE
                     ATAREVEAHGPAAAGAGADVSDEPAVQAAIARIVERLGPIDILVNNAGIDTTHEVDGM
                     PTDMWDRMVDVHMKGTFLCTREVLPAMKAKGWGRIINISSQLAHKGGRTMAHYCAAKA
                     GIMGFTKSLAYEVAPFGITANTINPGPVDTPLLQALPKEWLAMKKGELPIGRFGRVEE
                     IAPAALLLASEEGSFFVGASLNMNGGDYMI"
     gene            complement(12221..13300)
                     /locus_tag="P5558_14120"
     CDS             complement(12221..13300)
                     /locus_tag="P5558_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25497.1"
                     /translation="MPLLTVQDLVQHFPIAGSKKVVSAVNKVSFDLEPGETLALVGES
                     GSGKTTIGRCVLGLIRPTAGRIRYRDIEMGGRKTVRSPELRGKLQLVFQEPAESLDPR
                     LRLFEQIDEPLRALRVNRADRERQVRAAAARVGLREAMLGLYPAEISMGLQQRVGIAR
                     AMISEPEIVVLDEPTSALDPTARAEIIDLLIRIQRDLGTAYLFISHDLSAVRQISHRV
                     AVLYLGMIVEQGPAAKLFARPSHPYSVGLLSAVLLPNPTIRRESRISLAGEIPSPIDL
                     PRACYLASRCPMVEARCRARMPEVETVEPGHEVRCYRHEEVVRDADAVDYFARFQAEA
                     ERILGAGMPPQVVAGQPMKGNDDHG"
BASE COUNT         2043 a         4848 c         4581 g         2065 t
ORIGIN      
        1 cgggtcgaac gggtcggaat agctgtcgcc gaagacgaag agccggccca gcttgtccgg
       61 gctctcgctg ccggccgtcg cataagggtt gggaacggcg aagtcgacga ccggcggcgt
      121 gccgccgccc ccgccgccac cgccaccgga gggcgtcgac ggcgtcccgc cgccgctacc
      181 cccgccgccg ccgctgcagg cggacagagc cagcagcgcc atcgcgctga aacccgagat
      241 cgaccagtgg ttcctgttaa tcattgcacc tatcctgcgt tagtcatcgc ggctcgtgcc
      301 tgccgctatt tattgatttc gcagggatca agtctataaa aagtccgtat agacggcgcg
      361 gggtcaaaat aaatctacag tttattatta accatctcga tatttcgtgc agccttcgcc
      421 gaaccgcaca agcatttgga gcaacgacat ttcgctggtt aaccggagcg ccatcattaa
      481 tattgtgttt cgccggccga cggcgacgcg gcggcgaccg aaacagcatc gggataggcg
      541 caaatattaa ggcctggcgc cctgggcggt taacgcgcgg cgctcctggc ggccattgac
      601 gttaacgccg ggcccgtccg ggcgacctac cggcgctcac cagcgtcccg gctgcaggac
      661 ggcgacctgg cgctgcggca gggtgatgcg ggaataggcg agactcgagc cggcgaacgt
      721 caccacgtcc cagtcctcgc ccatcagcgc caggaaccgg tcgagcgagg ccgtcccgaa
      781 atagtgcatc gggatcacca ccgacggccg gatctgctcg atgacctcct tcatcgcggc
      841 ctgcgccagc gtgtagctgc cgtcgaccgg caccagcagg atgtcgatga cgccgagctc
      901 gcccagatgc atgtcggtca gcacgtgatg caggtggccg agatgggcga tgcacagatc
      961 ctcgacctcg aagacgaaga tcgagttgct gttggcctgg gcgccgctcc gcccgtgcac
     1021 cgaggtcggc acgttgcgga tgcggaggtc ctcgagctcc aggtcgtgct cggccacccc
     1081 gccttccggc ttccagccac gcagcacatg ccggatcccg gggtccggca aggtcgtgaa
     1141 atgcgtcgag tgggcgttgt tcatggtgac gatgtcgggc agcgccgggc ccgtgtgatg
     1201 gccgttgaag tcggtgatca ccgtggtccc gccggtcgtc tcgatccaga agctggaatg
     1261 gccgagatag gtgaggccga ccgtcgcacc gtccggcagg ctggcgagcc gcacgccggg
     1321 cagcaggcgc tcggcgatcg gaaagcagcc gcgcgcctcg gccggcggtg ttgccatcgc
     1381 caggcccagg agcgtcagga gcccacaggc gaaacgggtg aagccacggg acttgcgcat
     1441 ttaccctctc cctctcgtcc gacagccctt tccggccccc ccggatcgcg gctatgtacg
     1501 ggcacgatgc tgcaccgctc cctcatccga aatgtctcga ttctggcgac gtgccaggcc
     1561 tttttcttca tggccaacac gatccagatc acgacctccg ccctggtcgg gctgcagatc
     1621 gccccgagcc cggcgctcgc caccctgccg ctcgcggcgc agttcgtcgg caacatgctg
     1681 gcgaccatgc cggcctcgct gctcatggca cgcatcggca ggcgcctggg gctcctcctc
     1741 ggcagcctct tcggcgtgct ctccggcctg ctcgcgacct gggcggtggt gcaggcgagc
     1801 tacccgctct tcatgaccgc cggcctgctc tacggcgtct tcggcgcgtt cagccagtat
     1861 ttccggttca ccgccgccga tgcggccgat gccagcgacg cccgggacaa ggtggcggcc
     1921 cgggcgcggg ccatcgccct cgtgatggcc ggcggcctcg tcgccgccgt gctcggcccg
     1981 gagctcgcca aggcgacgca ggacctgctg ccgcccttcg tcttcgccgg ctgctacctg
     2041 atgatcgcca ccgtggcgtt ctgctgcggc gtggcgctgg ccttcctcga cctgccgcac
     2101 aaggtggcga cgaccagcga cgcgccggcc cgcccgctcc tggagatcgt gcgcaagccc
     2161 gaggcgatca ccgccttcgc cgccgccctc gtcgcctatg tcacgatgaa catgctgatg
     2221 accgcgacac cgctcgccat gctcgcctgc ggctatgcct tcgccgacag cgcgatggtc
     2281 atccagtggc acgtggtcgg gatgttcgcg ccaagcttct tctccggctc gctgatcgcc
     2341 cggttcggcg ccgagcggat catcgccttc ggcggcctgc tcatgctcgc ctgcatcggc
     2401 accaacctcg ccggcatcga ggtcgtcaac ttcgtcgcca gcctgctgct tctcggcctg
     2461 gggtggaact tcatgttcat cggcggcacg acgctgctga cgcaggccta ccggccgtcg
     2521 gagaaggcca aggtgcaggg gttcaacgac ctctgcctgt tcagcgccgt cgccgcctcg
     2581 gccgcggcca gcggcgccct gcacgaatgg ctcggttggc agccgatgaa catcgcggcc
     2641 ctgccgctcc tgctcgccgt catcctcctc gcggtgatcc gggccgcccg ccggccgctc
     2701 gccgcacccg tctgaaacgt ccagccagcc cagcaaggaa cccccttgag cgccttcgcc
     2761 gaccatctcc agctcagcaa gcacgccgca cgcggcaggc acggcgtggt cgccgcccag
     2821 aacctgaagg cggcgacggt cggtgccgag gtcctgaagg cgggtggcca tgcgatcgac
     2881 gccgccgtcg cgacctcgct ggcgctggcc gccaccgagc cctggatgag cggcctcggc
     2941 ggtggctcga ccatgctgat cctcgaccgg gcgaccggcg aggtcgagtc catcgatgcc
     3001 ggcatgatcg cccccgcggc cctcgacccc gccgcctacc cgctcgtcgg cggcacggcc
     3061 ggcgacctct tcgcctggcc cgccgtggtc gaggaccgca acgtgctggg ccccctctcc
     3121 ttcgccgtgc cgggtcatct cgccgggctc ggcctggcgc acgagcgcca aggccgcatg
     3181 accttcgccg agctcgtcgg cccggcccgg caactggcga aggacggcct cgagatcgac
     3241 tggtacgcgg cgctgctggt cggcaccgcg gcccgggatc tccgccgttt tccctcgagc
     3301 gccgaggtct ggctcgccga cggcctgccg ccgaccccgg ccgtcgccgg cgagccgctg
     3361 aagctgccgc tcggccggct ggccgagacg ctggatctcc tggccgagga ggggccgaag
     3421 acactctaca cgggcggcct cgccgagctg gtcgcggccg acgcggcggc gctcggcagc
     3481 ccgctcgcgg cggccgacct cgccgcctac cgggcctccg tggcgcctgc cctgcgcatc
     3541 ccctacggcg aggccgaggt cttcggcatg cccggccttt tcgccggcag cacgctgcgc
     3601 cgctgcctcg aagccctggc cgggtggtcc ctggcgagcg attcgccggg cagggacgcc
     3661 tatctcgcct tcgccgaggt gctgaagggc gcctacgcca agcggctcgc cgaggacggc
     3721 catgccagcg acggccccgc cccttcctgc accagccacc tctcggtgat cgacgccgaa
     3781 ggcaacatgg tggccctcac ccagaccctg ctctcgctct tcggcagccg ggtcgtgctg
     3841 ccgtcgaccg gcatgctcat gaacaacggc gtcatgtggt tcgaccccag gcccggccgc
     3901 ccgaactcga tcgcgcccgg ggtccgcccg ctctccaaca tgtgcccggt gatcgccacg
     3961 gatgccacgc gccggctggc gctcggcgct tcgggcggca ggcgcatcct gcccgccgtc
     4021 ctgcagctcc tgagcttcat cctcgacaac cacatgtcgc tcgaggaggc tttcgccgag
     4081 ccgcggatcg acgtctcggg cgacgagctg gtcaccatga gccgcgacct cgacgaagag
     4141 gtgcgggcgg ccctcgccga ggcgcacggg gcgacgacgg tgcgggccga ggtctatccg
     4201 atgcgcttcg cctgcccggt cgcggtcctg cacgacctcc tgctcgacga ccgctacggc
     4261 accgccgagg tgatccatcc ctgggccgat gccgtggcgg tgcaatgagc cggcttggcg
     4321 ccgggggccg cttgtgctga actatgatgc gttgcaacaa aggggacgct ggatgcgcaa
     4381 gctcgcggga ctgatcggca tggcggggct cgccctgggc ctccacctcg ccatggtcga
     4441 gaatgccacg gcagcccaag accagctcgt catgggcatg gtgctcgagc cgccgcatct
     4501 cgatcccacg gccggcgccg cggcggcgat ccgcgaggtc acttacgcca acatcttcga
     4561 gggcctgacc cggatcgacc ggagcggcag cgtgcagcct gctttggccc gctcgtggac
     4621 gatctccgat gacgggctga cctacacctt cgagctggcc gacggcgtga ccttccacaa
     4681 cggcgtgccg ctcctcgcgg agcatgtcgt cttctccttc gagcgggcga tggccgagga
     4741 cagcgtcaac gcgcagaagg gcctgttcga gccgatcgcc agcgtcgagg cgccggacga
     4801 cgggaccgtg gtgatcacgc tcgagcgccc gaccggcagc ttcctcttca atctcggctg
     4861 gggcgacatg gtgatcgtcg agcccggctc ggccccgacc aacaagaacg agccggtcgg
     4921 cacggggccc ttccgcttcg tccgctgggt caagggcgac cgggtcgaga tcgagcgcaa
     4981 tccggactac tggggcgagg cacccgcttt ggcgaaggcg acgttcaagt tcatccccga
     5041 cccggcggcg gcgaccgcgg ccatgctggc cggcgacatc gacgccttcg ccaacttccc
     5101 ggcccccgag gcgctcgtcc agttcgaggc cgatccgcgc ttcgtcgtcg tcatcggctc
     5161 gaccgagggc gagaccatcc tcgccatcaa caacgcgaag ccgccgctcg acgacatccg
     5221 cgtccgccgg gcgctggccc atgcgatcga ccggcaggcg atcatcgacg gggcgatgat
     5281 gggctacggc acgccgatcg gctcgcactt cgccccgcac aacccggcct atgtcgatct
     5341 caccggcacc tatccctacg acccggaaaa ggcgaaggcg ctgctcgccg aggccggggc
     5401 ggaggacctc gagctgaccc tcaagctgcc gccgccctcc tacgcccggc gcggcgggga
     5461 gatcatccag gccgagctcg ccgccgtcgg catcgaggcc gagatcatcc cgatggaatg
     5521 ggcgcagtgg ctcgagcagg tgttcaccgg caaggactac gacctcacca tcgtcagcca
     5581 caccgagccc atggacatcg gcatctacgc ccggccggac tactacttcc agtaccagag
     5641 cgaggcgttc aacgaggtga tggacaagct cgccgccgtg accgacgcgg cggagcgcca
     5701 ggcgctgctc ggcgaggcgc aggagatcct ggcagaggac agcgtcaacg tcttcctctt
     5761 ccagctcgcc aagcacggcg tgtggaaggc cggcatcgag ggcctctggc acgacagccc
     5821 gatcccggcg aacgacctga ccgaggtcca ctggaccgag tagggcgcac ctcaccacca
     5881 tggcgacacc catccagata gggccgctca ccctcgcctc gcctgtcctc atggcgccga
     5941 tgacggggat cagcgacctg ccgttccgcc ggctggtggc gaggttcggc gccgggctga
     6001 tcttctcgga gatgatcgcg agcccgcatt tgctggccga aaccgccaag acccggcgcc
     6061 aggcgcgctt cgatgcggct tcgcggccct gggcggtgca gctcgccggc accgagccgg
     6121 ccgtgctggc ggaggcggcg cgttacgtcg tcgatcgcgg tgccgatctg gtcgacctca
     6181 atttcggttg cccggccaag ctcgtggtcg gcaaggccgc gggctccgcc ctgatgcgcg
     6241 acgaggcgct ggccggccgc atcctggatg ccgtggtcga ggccgtgccg gtgccggtca
     6301 cgctcaagat gcggctcggc tgggacgacg gcaatcgcaa cgcggcgagg gtcgcccgca
     6361 tcgccgaaga ggccggcatc cggatgctga ccgtgcacgg ccgcacccgc tctcagctct
     6421 tcaagggtgc ggcggactgg gcggcggtcg ccgaggtgaa ggcggcagtg ggcctgccgg
     6481 tcgtcgtgaa tggcgacatc accgatgccg ccaccgccag ccgcgccctc gccctctcgg
     6541 gggcggatgg cgtcatgatc ggccgcggcg cctgcggccg gccctggctg ctcgcccaaa
     6601 tcgcggcagc gctcgatggc gccggcgagc cggcggaacc gacccgggac gagatgctgg
     6661 ccatcgtcct cgagcaccat gcggcgatgc tcgggcatta cggccgggac ctcgggctcc
     6721 gccatgcccg caagcacctc gcctggtatc tcgccgccct gcccggcggc gccgggctgg
     6781 cccgccgggt caaccggctg gacgatgcgg aagcggtggt cgccgcgttg cgcgagatct
     6841 atgcttcggc agcggacacc gaacccgcgc tcgcggcctg atggcaagcc ctaccccttc
     6901 gccattcaga gccatcgtag tagatcgtcc gtgagcatgc ccgttgcctg gagttgactg
     6961 gaatgcacat cgccaggatg gccgcggccg ccctctcgat cctgctggca ctgaccacag
     7021 tcgaggcaag gggccagggc gaggcgcccc tcttcagcct ggacttcgcc gactaccagc
     7081 ccgccgacgg ctcggttcgg gactggctcg ccggccgggg cttcgatttc gccaaggcgg
     7141 ccgatgacga caggaagatc agcgtctccg tcgaggacgg tgtcctgcag gtcgacacga
     7201 agaagcaggc gttcggcgtc atctaccacg agggcgacgt gccccgcgcc acccggctcc
     7261 gcgtcgcctg gggcgtggac gtcttcccga aggacgtcgg ctacgagaag gccgtcaaca
     7321 acgaggcctt gatgatctac gtcttcttcg gccgggagaa gaagccgtcg ggctccgtct
     7381 tcattccgga cgtcagctac ttcatcggct tctatctctg cgacagcgac cgggtcgatc
     7441 acccctacaa aggccgctat caccacgaga gcggccgctt cgtctgcctc gcccatccga
     7501 agcccggcga gaccatcgtc tcggaggttg acctgagggc ggccttcgag agctatttcg
     7561 gcagggccga catgccgacg gtctccggca tcagcctcga ggtcgacacc tccgatgccg
     7621 gcgacggcgg caaggccagg gcgttcctcg agaaggtcga gctcctgccg tagcggccgc
     7681 ggcccgggat caggtcgcga ccgcgcccgc cttgccggtc accagcgtcc attcgaccag
     7741 ccgcttgagg accttgccga gcgcgtcgtc gaaggcggcg acgatcgccg gcaggtcgcc
     7801 ggccgcggcc ggcaccgtgc ggccgatctc ggtggtgccc accacgtcgc gccgcggcat
     7861 ggcgatcagc ttggcggaca tcaccacgcg agcctccggc ggccccgatt ccacttgcag
     7921 cgcctgaaag gcgatgatgt ccgtctgcag catgaagtcg gggcgcacct ccgagcgccg
     7981 gtcggtcacg accgcgatgg cgcccgagct cctgaaggac tggatgatca agggctggat
     8041 catcgccggc ggccgatcga cccaggccgc atcggcgtag aactcgacct ccacaccgct
     8101 cacgcgggcg atccggttgg tgtcgatcga ccggtcgacc gtcggccggt cgaccgccag
     8161 cgcccagtcg acctgcggca gctcgtcgaa cgtcgtcttg ggcgtcaccc ggaactggcg
     8221 cggcggcgcc gactggcccg gcagctggca ggcgccgagc gcgagagcgg gcagcgcggc
     8281 gcccatgaag atcagcccgc gtcgatcgat ccttgcccgg tttcgcatcg cgtccccttc
     8341 cctccgttgc tgccgtccac gggccgcagg cgccggcccc gcctattccg cccgcacacc
     8401 ctgggcccgg tcgccgaaca ggaagcgggc gggatcgcgc tccagatcct ccatcacccg
     8461 cgtgatctgg tccaccatcc gctgcgcgtc ctgcgccagg ccggtgatct cgtagaggcc
     8521 ggtgccggtg aagtcacgca tgccggcacg gttctcggcc accatgttgt tgacctgatc
     8581 ggccatcttg cgcagcgcgg cgacgctctc ctgcagatcg gccagcgtct tcgccaggcc
     8641 tgcggcgttg tcctcgagca agccgttcgc ggtgtccgcc gtgcgggtca ccgcggcggc
     8701 ggtcccggtc gattcgtcga cgagcgccgc cagcttgggc gtgaccgcgc tcaggtcctc
     8761 ggccatcctg gcgaagctgt cgaagacgct ggcgagccgc cccctgttct cgctggcggc
     8821 cgtgccgagg ctgccgaaaa cctcgttcat cttgtcgagc gatgccgtca gctgctgcgc
     8881 gatctcgccg gcgttggcct gcagcagctg caggtccgac tggatggtcg gcagctcggg
     8941 atagggcagc tcgaactgcc ccttgttgtg gaactccggg ggcggcgcgt cctcgaagaa
     9001 atcgaggttg accgagagca gcccggtcac gaagctctcc agcccgagct gggcgcgaaa
     9061 gcccttgtcg aacaggctcg ctcgaaacgc atcgtcgtcg acggtcaggt cctcgccgac
     9121 ggcgacgatc cgatcgcgat ccatctccag gatcaccggg atgcgcgcct ggacggtttc
     9181 cggctcgaac atcactttga tctcggtgac ctggccgaca ttcacgccgc ggaagttgac
     9241 cggggcgccg acgcgcaggc cggcgatcga gcccggaaag taggcgacga gcggatggcg
     9301 ctcctggaac catcgggtgc cgccgaagac gacgatgcca agggcgagga gcgccaggcc
     9361 gcccagcacg aaggcgccga tcacactcgg gctggctttg ctgctcatgg ctgcgcgtgc
     9421 tcctgcggtg aacgttcgcc cccctcgccg ccgccgcggg tcaggaactc gcgaaccttg
     9481 ggttcctccg tctcggcgag cagccgcttc ggatcgcccg tggcgagctg ggtgcgggtc
     9541 tccgcatcga ggaagacgga gttgttggca atcgcgaaga tgctgggcag ctcgtgggtg
     9601 accatgacga tggtggcccc caggctctcc cgcagctcca ggatcaggtc gtcgaggcgc
     9661 ttggagctga tcggatcgag cccggccgag ggctcgtcga agaacagcac ctccgggtcg
     9721 agcgcaatgg cccgggcgag gccggcgcgc ttgcgcatgc cgccgctgat ctccgagggg
     9781 tagtagtcct cgaagccggc gaggccgacc agcgccagct tgaacgagac catgtcgcgg
     9841 atctcggcgg ccgaaagatc ggtgtactcg ccgagcggca gggcgacgtt ctccgccagc
     9901 gtcatcgagc tccagagcgc cccctgctgg tagagcacgc cgaagcgctg catcaggttg
     9961 gcgcgggccc gctcgtcggt tgcccagaag ctggcatcgc catagcgcac ctcgcccctt
    10021 gccggcgcct tcagcccgac catggcgcgc atcagcgtgg tcttgccgca gcccgacccg
    10081 cccatgatga cgaagacgtc gcccttctgg atgacgaagt cgatgtcctt catcagcacg
    10141 aagctgccat aggcgatggt cagcccgcgc acggcgatga acggcgcctc agatgccgag
    10201 ggcatcgtag atcaccgtga gtgccgccat ggagaggatg atgaagacga tgctggtgac
    10261 cacggccgag gtcgtggcgt tgccgaccgc ggcagcgctc cgaccgcact gcatgcccct
    10321 gaggcagccg cagaccgcga cgacgacgcc gtagaccgtg gccttgagca ggccgcccgc
    10381 gaagaagagc aagggcgtcg aggcctgggt ctgctgcaga tagagggcgg ccggcaggtc
    10441 gagcatgacg acgccgacga agaggccgcc caggatgccg agcaggttgg cgtagatgca
    10501 gagcaggggc gtcatcagga tgagcgccag catccggggc aggacgagga actccatcgg
    10561 cgacaggccg agcgtcgaga gcgcgtcgat ctcctcgttc acctgcatgg tgccgagctg
    10621 cgccgcgaac gccgcccccg tccgcccggc caggatgatg ccgaccatca tggcggccat
    10681 gtcgcgcgcc atgccgatgg cgacgagatc ggcgacgaag atctgcgccc cgaactgcag
    10741 gagctggatg gcgccgagga aggccaggat cacgccgacg aggaagctga tcagcccgac
    10801 gatcggcagg gccttcgccc ccacctcctg cacgacctcg aagaaatccg accagcgaaa
    10861 gcgggcccgc ccgcgcacga ggcggccgaa cgcgatgacc acctcgccga tgaaggcgag
    10921 cgtttcgcca caggcctcga ggaacgcctg cgtgcccttc cccacccgct cgagcaaggg
    10981 cggcctcgca gccgccttcc gcgcccccgc ccgctccggc acgctggcgg cgaggccgag
    11041 aagccgtgtc gccccctcgg gcaggctgct cgtgtcgagc ttcggcccat cggccgccgt
    11101 cagccgctcg agcttgcgca ggaaggtcag gaacgccgtg tcccaggcgc cgagccgcgc
    11161 cgtgtcgagc cgcacgctcg tcaccgcgtc gcccttgagc tcggcatgca cgcgctcggc
    11221 gctcggcacg ccccggcggg tcagccagtc gccgaacagc acgagccgca acgaccccct
    11281 gcccgtgggg tcgcccacga cctcgaagcc cgccgtctcg tcgttgctct ggatcgccgc
    11341 cgccatgagg cttccgaaaa aatcgagcgt cgccacctgc ccgcttcgcc ctactacgga
    11401 cgggcgcaac gcagaacagg ccgaacgtca ccggccggtc tttatccccg cactgtgacc
    11461 ccggcgcggc gcgccgaaac ctcagatcat gtagtcgcca ccgttcatgt tcaggctggc
    11521 gccgacgaag aacgagccct cctcggaggc gagcaggagg gcggcgggtg cgatctcctc
    11581 gacccggccg aagcggccga tcggcagctc gcccttcttc atggcgagcc actccttcgg
    11641 cagcgcctgc aggagcggcg tgtcgacggg cccgggattg atcgtgttgg cggtgatgcc
    11701 gaagggggcg acctcatagg ccagcgactt ggtgaagccc atgatccccg ccttggcggc
    11761 gcagtagtgg gccatggtcc gcccgccctt gtgggccagt tgcgagctga tgttgatgat
    11821 gcggccccag cccttcgcct tcatcgccgg caggacctcg cgcgtgcaga ggaaggtgcc
    11881 cttcatgtgg acgtcgacca tgcggtccca catgtcggtc ggcatgccgt cgacctcgtg
    11941 ggtggtgtcg atgccggcat tgttgacgag gatgtcgatg gggccgagcc gctcgacgat
    12001 acgggcaatg gccgcctgca ccgccggctc gtcgctgacg tcggcgccgg cgccagccgc
    12061 ggcgggaccg tgcgcctcca cctcccgggc ggtggcctcg gccctggccg cgtcgagatc
    12121 gacgatgccg acccgtgccc cctcgcgcgc gaagagatcg gcgatggcgc ggccgatgcc
    12181 gctgcagccg ccagtgacga gcgccgtccg gccgtcgagc ttacccatgg tcatcgttcc
    12241 ctttcattgg ttggccggcc accacctgcg gcggcatccc ggcaccgagt atccgctccg
    12301 cctcggcctg gaaccgggcg aaatagtcga ccgcgtcggc gtccctgacc acctcctcgt
    12361 ggcggtagca gcgcacctcg tggccgggct cgaccgtctc gacctcgggc atcctggccc
    12421 ggcagcgcgc ctcgaccatc gggcagcggc tggcgagata gcaggccctg ggcaggtcga
    12481 tcgggctcgg aatctcgccg gcgaggctga ttcggctctc gcgccggatg gtcgggttgg
    12541 gcagcagcac ggccgagagc agcccgaccg aataggggtg gctcggccgg gcgaacagct
    12601 tcgccgccgg cccctgctcg acgatcatgc cgagatagag gaccgccacc cggtggctga
    12661 tctggcggac cgccgagagg tcgtggctga tgaagagata ggccgtgccg aggtcgcgct
    12721 ggatgcggat caaaaggtcg atgatctcgg cccgggcggt cggatcgagc gccgaggtcg
    12781 gctcgtcgag cacgacgatc tcgggctccg agatcatcgc ccgcgcgatg ccgacgcgct
    12841 gctgcaaacc catgctgatc tcggccggat agagcccgag catcgcctcg cggagcccga
    12901 cccgggccgc cgccgccctg acctgccgct cgcggtcggc acgattgacg cggagcgccc
    12961 gcaggggctc gtcgatctgc tcgaacagcc gcaggcgcgg gtccagcgat tcggcgggct
    13021 cctggaagac gagctgcagc ttgcccctga gctccggcga gcggacggtc ttgcggccgc
    13081 ccatctcgat gtcgcggtag cggatgcggc cggcggtcgg gcggatgagg ccgagcacgc
    13141 agcggccgat ggtggtcttg cccgagccgc tctcgccgac cagcgccagc gtctcgccgg
    13201 gctcgaggtc gaagctgacc ttgttgacgg ccgagacgac cttcttgctg ccggcgatgg
    13261 gaaaatgctg gacgaggtcc tggacggtga gcagcggcat ggcctaggag cctgtccggg
    13321 aaatggtcat ggcctgcatc gcttcagggc aagcgagggc aaggagcggc ggcgatggcg
    13381 atgccggggc atcgtcgagc cggcgcgacg ccgccctcgc ttgccctgag gcgacccgaa
    13441 gggcacgcca gccctggctc cgggccacgt cggccggccc tgacgatggc cccgcatcgc
    13501 ggcgggccgg tctcctcgcc caaagccagg gctggcg
//
LOCUS       DAOTFI010000132        13532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5443_length_13532_cov_6.939972, whole genome shotgun sequence.
ACCESSION   DAOTFI010000132 DAOTFI010000000
VERSION     DAOTFI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5443_length_13532_cov_6.939972"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="P5558_14125"
     CDS             complement(<1..1362)
                     /locus_tag="P5558_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRY25498.1"
                     /translation="MTALAEARRQWLANPRGDILAGIVVALALIPEAIGFSIIAGVDP
                     KVGLYASFSIAVITALVGGRPGMISAATAAVAVLVVPLVREHGVEHLFAATILMGVIQ
                     VIAGLLRLDLLMQFVSRSVITGFVNALAILIFVAQVPQLIGVTPATYVMVAGGLAIIY
                     ILPRFTRVVPSPLIAIIVLTVIAIQGGIDVNTVGDMGQLPSTLPYFLLPDVPLTLETL
                     QIILPFSLTMAAVGLLESMMTAQIVDDLTDTPSDKRRECKGQGIANFVTGFLGGMGGC
                     AMIGQSVINVKSGGSTRLSTMVAGCCLLFLIVVLGPLVSRIPMPSLVAVMIMVSIGTF
                     SWKSIRDLRSHPWQSSVVMVVTVVVVVWTHDLAQGVVSGVLLSGLFFAGKVRQLFTVT
                     SELSDDGLERTYRVAGQVFFASAERFAEAFDFKEVIDRAIIDVSAAHFWDISAVGVLD
                     KAIL"
     gene            complement(1573..2799)
                     /locus_tag="P5558_14130"
     CDS             complement(1573..2799)
                     /locus_tag="P5558_14130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY25499.1"
                     /translation="MDGSPLGLKRDIRVIGVVGAAHGGSHFFHLVLPPLFPWLREAFD
                     VSYTELALVMTVFFAVSALAQAAAGFVVDRIGAAKVLTFGLASLGVGAVLIAMAPGYW
                     ALPPAAILLGLGNSVFHPADYAILNHHITPNRMARAFSVHTVGGTLGWALAPLVMASS
                     AALVSWRFALVLAAVIALLLTLLVLFSRRQLETPKQQRNTDAADRRQQRGALIGLFLS
                     GPILLCFAYFALQASGFLAVQSFMPLALHELFGTPMVIATGTITAFMLGSAMGTLVGG
                     VIADASGRFQLMIAIGLALSACCLLLVGYVPMEPALLFGVLAIAGSLQGTTTPARDML
                     VRSATPPGSTGKVFGFVYSGLDLGATIMPLVMGWLLDHGHAQAFFPLVAAVTLLTILT
                     ATSIPNRRGTAPAAAE"
     gene            3054..4091
                     /locus_tag="P5558_14135"
     CDS             3054..4091
                     /locus_tag="P5558_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY25500.1"
                     /translation="MKVLVTGSDGYIGRVLVPLLQRAGHEVVGLDSRLYDGCDFPGLE
                     APGLPTIVKDLRAVERADLEGCDAVLHLAALSNDPLGDLDPDITYDINHKASVRLAEL
                     ARDAGVRRFVFSSSCSNYGSAGDDYLDETATLAPVTPYAESKVWTERDVAPLATDRFT
                     PTFLRSATAYGLSSRLRGDLVVNNLVGYAATTGHVLIKSDGMPWRPLVHIEDIALAFK
                     CVIEAPVEAVRNEAFNVGQTSENFRVREVADMVAEVVPGSSVTYEGGASPDVRNYRVN
                     CDKIRRVLPAFRPAWTLRAGIIQLYEAYRKVGMTKEQFLGSRYFRIGHVKEMIAKGAL
                     DTRLVPRSSGG"
     gene            4157..5263
                     /locus_tag="P5558_14140"
     CDS             4157..5263
                     /locus_tag="P5558_14140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY25501.1"
                     /translation="MNSCLLVDDRQTALDFPTGDLELAFCAACGFIQNRRFDPAAVTY
                     STDYEETQGFSGHFRRFVDELIDDQDSKHHLAGKTVLEIGCGKGEFLSRLVERTGARG
                     VGIDPSFRPDRLAGGATDRPTFIQDWYGPAHADLAADFVCCRHTLEHIPDVLDFVRLV
                     RRGIGDRPGVRILFEVPDTRRVLAELAFWDIYYEHCSYFTAGSLARLFARAGFVVDDV
                     RTAYGDQYLLLEARPADGSSRPGKSVDDLAETAALVEQFASGLPRKLEALRAEVDAWR
                     GAGRTVAIWGSGSKAVAYLTSLGLATEIAAVVDINPHKAGKFMAGTGHRIEQPEHLRE
                     LRPDVVVAMNPVYLAEIGQALGELGLAPELRALD"
     gene            5274..6713
                     /locus_tag="P5558_14145"
     CDS             5274..6713
                     /locus_tag="P5558_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRY25502.1"
                     /translation="MTLAATLGSRFALLLGGRVALLVLALVSTAMLTRILEPTGFGHY
                     RTAMAYLALVIALADLGLSSLFVREISRPGADQPRLIANLLGLRLALAGAALAFASGL
                     AFLLPLDAQGRLGIVGGSFGFLAYSLHLLLFGLFQQKLRQQGVVLAEITGGLVLVGLI
                     IVFAWAGAEPFWFVVAMGLSYIATLALTFVAAQRLVRFGLRLEPALWLDLARQAAPLA
                     VATTISVLYFRADTVLLAFFRAPAEVGLYGVPVKVLDSCMGISMLLVGLFTPLMAHSA
                     RVDEVAFRDHLGTGLLTLAAGTTLVGVGLVALAPEIVTLLAGAEFTGGASILQVLAGV
                     LVLHGTAVILREAATALTIQQRLLPAYFAGLGVAMVAYVMLIPRFGGIGAAAALLLAE
                     IVVVLLTGSIVARAATGLAVMRRPLMALGCGLVAAVLTVVAERSGVHWFLRGLLAGVT
                     YLMLLLGTRTLQLPALISLGRDMLARRNA"
     gene            6762..7631
                     /locus_tag="P5558_14150"
     CDS             6762..7631
                     /locus_tag="P5558_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25503.1"
                     /translation="MKLSELVGHELWEKLLADRLGEPLHLNVASLFVKLFGGFRARVA
                     FDLIERRRYAFPLLKAADYAKAQGIGKIYVLEFGIAAGAGLVNLAWLAEQVTAETGVE
                     IAVVGFDAGTGMPRPKDYRDYPEEFIEGDFPLPDREALARVLPSGVRVVYGPVADTVV
                     DFIATLDAPVGFAVLDLAYYSSTVEALAALAGPAERYLSMTLVYLGAIRIDNANPAVG
                     ELLAVREFNAARPLRQIHPFTSLRDKRVFRRAAWLDQIHVLHVLDHPRRAGMAPLVRE
                     AWLLEDPSRQPAR"
     gene            7871..9130
                     /locus_tag="P5558_14155"
     CDS             7871..9130
                     /locus_tag="P5558_14155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY25504.1"
                     /translation="MAELGPLCRSCGLELVHEVLDLGMSPLCESFLRGDQLDRAEAFY
                     PLKVLACAGCGLVQLREYVSPDEIFGEYAYFSSFSESWVRHAAEYCRTMHGRLGLGGS
                     SLVVELGSNDGYLLQHFGELGVPVLGIEPAANVAAAAEARGVPTRVAFFGTKVAGELL
                     AEGRAADLIVGNNVLAQVPDLNDFVAGIALLLKPTGTATLEFPSLEQTIAGNQFDQVY
                     HEHFSYFSLIAVEALVARHGLVVADVEELASHGGSLRVHLRQAREASGIAPRVEALRA
                     RELAAGCADPARLAEFGSVVEATKRRLLSFLIEAREAGKRVVGYGAPGKGNTLLNYCG
                     IRTDFLDYLVDRNPYKHGRFTPGTHIPIFPVERIAETRPDYLLILPWNLRREIVRQMA
                     PVIAPWGGRLVVPIPKLEVIEATEVAA"
     gene            9127..9918
                     /locus_tag="P5558_14160"
     CDS             9127..9918
                     /locus_tag="P5558_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HRY25505.1"
                     /translation="MKVVLFCGGLGTRIRDYSENVPKPMIPVGQQPILWHVMQYYAHY
                     GHRDFVLCLGYKANVVKEFFLNYRPTATRDFIVSNHGQSIELLGEPEPDWRVALIDTG
                     MWRNVGARLWAVRKHLQNESMFSANYSDGLTDVNLPEMVEFFEASGKVACFLAVQPNF
                     SYHLVDFDESGHVAALRTSNQSEIWINGGFFLFRPEIFDYMRPGEELVVEPFRRLIEA
                     DELVAYRHRGFWASMDTLKDKQMLEDLFERGETPWLPWLQQAKPA"
     gene            9981..10598
                     /locus_tag="P5558_14165"
     CDS             9981..10598
                     /locus_tag="P5558_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRY25506.1"
                     /translation="MRLLCLGAHADDIEIGCGGFVLDRLARERPVHVDWCVLSASPER
                     ATEARASAAAFLADAASSEVTVAGFRDGYFPAERVAIKDWFEALKARPRPDIVLTHTQ
                     NDRHQDHQLVWQLTWNTFRDHLVLEYEIPKYEGDLSTPNLYVPFTEAVLARKVELLLE
                     HFPSQRRKPWFDTETFRGLARLRGLEAGSPTRFAEAFHARKLVLQ"
     gene            10613..11575
                     /locus_tag="P5558_14170"
     CDS             10613..11575
                     /locus_tag="P5558_14170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY25507.1"
                     /translation="MDDRPPTVSIGVPVYNGENFLGDAIESVLAQSVGDFELILCDNA
                     STDGTRAICEAYAARDRRIRYYRNPRNLGAAANYNRAFMLTRGRYFKWLAHDDRLHPA
                     FLEQTVAVMEARPEVVLCGSQVEVIDAQGAPIGTYGSILSQADVASPAERFAVYVLRP
                     HTGVDIFALIRRSALEGSLLHSHFHGADRALLAQLALRGAMVQIPATLNQIREHDQRY
                     TRRAKSARLRLDWHGSSPGGIAAIPILQLYQAYRRVVAGTAIATEERWRCRATLARWW
                     LVNWNSMRVAVDIAALVVPDIVGFAEGVKERLVGASAGHFKPPR"
     gene            complement(11599..13098)
                     /locus_tag="P5558_14175"
     CDS             complement(11599..13098)
                     /locus_tag="P5558_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRY25508.1"
                     /translation="MTGVSPARMISRDAPVAVVDIGSNSVRLVIYDTLSRAPFPRFNE
                     KSLCGLGSELDTTGRLPEAGIVACVNAVHRYCVIARAMQAERIDLLATEAVRRADNAG
                     ELVDAIRARTGREVRIVEGGEEAQLSAQGVAAGFHRPSGIVGDLGGGSLELSSVSEGR
                     VGSGAISLPIGALRVLPLMASDERAAKKHLDEIFANRPWVARDVERFFVVGGGWRALA
                     RIHVATVEAPLRVVHGLELDRDAARSLARSILRTAPDQVSALPGVPSRRAATVRAAAL
                     VLDRLLKAMAPERIVFSALGLREGWLHRCLPADERELDPLLVGAATFGRPRARVPHIG
                     EAMVHWTAPLFPGETEREQRLRIAACELSDIGWRDHAELRAQQSFHRLSQFPFVALDH
                     AERVFIALAVHRRYGGNDGEPGLAAAAALLDPALRQRAGVLGCAMRLGYRFSGSVPEL
                     LAQARMEVVDERVLLRLSTDEDVPDSEPVRSRLKDLAKALGMKGSDIVR"
     gene            complement(13126..>13532)
                     /locus_tag="P5558_14180"
     CDS             complement(13126..>13532)
                     /locus_tag="P5558_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRY25509.1"
                     /translation="SIIGRFLAHSRIICFGNGFGLPSRQAKVFISSADWMSRNLSRRV
                     ETLVPIENETVHQQIMSQVMVANLKDTAQSWLLNTDGTYSRLGSGSEAFAAHTYFMTN
                     PSLSGRGSALRKSKSRAQHLHLVPGHADDAGD"
BASE COUNT         2071 a         4622 c         4648 g         2191 t
ORIGIN      
        1 gaggatggcc ttgtcgagca cgccgaccgc cgagatatcc cagaaatgcg ctgccgacac
       61 gtcgatgatc gcgcggtcga tcacctcctt gaagtcgaac gcctcggcga accgctcggc
      121 cgaggcgaaa aagacctggc ccgccacccg ataggtccgc tccagaccgt catccgagag
      181 ctccgaggtc accgtgaaga gctgcctgac cttgccggcg aagaagagcc cgctgagcag
      241 cacgccggag acgacaccct gcgccaggtc gtgcgtccag acgacgacga cgacggtcac
      301 caccatgacc accgaggact gccagggatg ggagcggaga tcgcggatgg acttccagct
      361 gaaggtgccg atcgagacca tgatcatgac cgcgaccaaa gacggcatcg ggatccggct
      421 gaccagcggg ccaagcacga cgatcaggaa gaggaggcag cagccggcga ccatggtcga
      481 gaggcgggtg ctgccgcccg acttcacgtt gatgaccgac tggccgatca tggcgcagcc
      541 gcccatgccg ccgaggaacc cggtgacgaa attggcgatg ccctggccct tgcactcgcg
      601 ccgcttgtcg ctcggggtgt cggtgaggtc gtcgacgatc tgcgccgtca tcatcgattc
      661 gagcaggccg acggccgcca tggtgagcga gaacggcagg atgatctgca gggtctcgag
      721 cgtgagcggc acgtcgggca gcaggaagta gggcagggtc gacggcagct ggcccatgtc
      781 gcccaccgtg ttgacgtcga tgccgccctg gatcgcgatg acggtcagca cgatgatcgc
      841 gatcagcggc gacggcacca cccgggtgaa gcgcggcagg atgtagatga tggcgaggcc
      901 gccggcgacc atcacatagg tcgccggcgt gacgccgatg agctgcggca cctgggccac
      961 gaagatcagg atggccaggg cgttgacgaa gccggtgatc accgagcgcg agacgaactg
     1021 catgagcagg tcgagccgca gcaggccggc gatgacctgg atcacgccca tcaggatggt
     1081 cgcggcgaag agatgttcca cgccgtgctc gcggaccagc ggcacgacca ggaccgcgac
     1141 ggcggccgtg gcggccgaga tcatgcccgg tcggccgccg acgagggcgg tgatgacggc
     1201 gatcgagaac gaggcgtaga ggccgacctt cggatcgacg ccggcgatga tcgagaagcc
     1261 gatggcctcg ggaatcagcg ccagggcgac gacgatgccc gcgaggatgt cgccacgcgg
     1321 gttggccagc cattggcggc gggcctcggc gagtgccgtc attgagggtt ccagcaagat
     1381 gtcgggcggg ccggcacgcc ggggcggtgc cgcatcgcct ggatgaaagg ccggggcccg
     1441 aatcgccatg gccgcgccgg ccgggcatcg ccaggccacg ctgcccagcc tgccgggtcg
     1501 aacgaggcag ccagcctagc cccaaggggc tttatgctgc aactgctaac tgggtggcgg
     1561 ccggcccgtg gcctattcgg ccgcggccgg tgccgtgccg cgtcggttgg ggatgctggt
     1621 ggcggtcagg atggtgagca gcgtcaccgc cgcgaccagg gggaagaacg cctgggcatg
     1681 gccgtgatcc aagagccagc ccatgaccag cggcatgatc gtggcaccca ggtcgagccc
     1741 cgaatagacg aagccgaaga ccttgccggt gctgccgggt ggcgtggcgc tgcgcaccag
     1801 catgtcgcgg gcgggggtgg tggtgccctg cagcgacccg gcgatggcga gcacgccgaa
     1861 gagcagggcc ggctccatgg gcacgtagcc gacgagcagg aggcagcagg cgctcagcgc
     1921 caggccgatg gcgatcatga gctggaaccg gcccgaggcg tcggcgatca cgccgccgac
     1981 cagcgtgccc atcgcgctgc cgagcatgaa ggccgtgatc gtgccggtgg cgatgaccat
     2041 gggcgtgccg aagagctcgt gcagcgccag cggcatgaag ctctgcacgg cgaggaagcc
     2101 cgatgcctgc agcgcgaagt aggcgaagca gagcaggata gggccggaga ggaagaggcc
     2161 gatcagggcg ccgcgctgct gccggcgatc ggccgcgtcc gtgttccgct gttgcttcgg
     2221 ggtctcgagc tggcgccggc tgaagagcac gaggagcgtc aggagcaggg cgatgaccgc
     2281 cgccagcacc agggcgaagc gccaggagac cagggcggcg ctggaagcca tgaccagggg
     2341 cgccagcgcc cagccgagcg tgccgcccac ggtgtgcacg ctgaaggccc gggccatgcg
     2401 gttgggcgtg atgtggtggt tgagaatggc gtagtcggcc ggatggaaga ccgagttgcc
     2461 gaggccgagc aggatggcgg ccggcggcag ggcccagtag cccggcgcca tggcgatgag
     2521 gacggcgccg acgccgagcg aggcgaggcc gaacgtcagg accttggcgg caccgatgcg
     2581 gtcgacgacg aagccggccg cggcctgggc cagggccgag accgcgaaga acacggtcat
     2641 gaccagcgcc agctcggtgt aggagacgtc gaaggcctcg cgcagccagg gaaagagggg
     2701 cggcagcacc agatggaaga aatggctgcc gccatgcgcg gcgccgacca cgccgatgac
     2761 cctgatgtcg cgcttgagcc cgaggggaga cccgtccatg ccgcccgatg ccttctctgt
     2821 ccgtgcatgg tcttctacga cggcagcggc tgacgcgcca accgatgatc gtgcctgccg
     2881 ggcagcgggc gtcgctgcaa cactggcgcc ctcacgtgcc gccctgccat aagcgcactt
     2941 gaagccgatc gcctcgcccg tcgatactga caccacaggg tatccagggc gcggctcgtg
     3001 cgggctgcca atcttgtcag ccaaatcccc gtcaaataac ggaaagcggt tccatgaagg
     3061 tactggtcac cggcagcgac ggctatatcg gtcgcgttct cgtgccgttg ctgcagcggg
     3121 ccggccacga ggtcgtgggc ctcgacagcc ggctctacga tggttgcgat tttcccggtc
     3181 tcgaggcgcc ggggctgccg accatcgtca aggatctgcg tgccgtcgag cgagccgatc
     3241 tcgaaggctg cgatgcggtg ctccatctgg ccgccttgtc caacgacccg ctcggcgatc
     3301 tcgaccccga catcacctac gacatcaacc acaaggcatc ggtccggctt gccgagctcg
     3361 cccgcgacgc cggggtccgc cgcttcgtct tctcgtcgtc ctgcagcaat tacggctcgg
     3421 ccggcgacga ctatctcgac gagaccgcga ccctggcgcc ggtcacgccc tatgccgaga
     3481 gcaaggtctg gaccgagcgc gacgtggcgc cgctcgccac cgatcgcttc accccgacct
     3541 tcctgcgcag cgccaccgcc tacggtctct cgtcccggct gcgcggcgac ctcgtcgtca
     3601 acaacctcgt tggctacgcc gcgacgacgg gccatgtgct gatcaagagc gacggcatgc
     3661 cctggcggcc gctcgtgcat atcgaggata ttgcgctcgc cttcaaatgc gtgatcgagg
     3721 cgcccgtcga ggcggtgcgc aacgaggctt tcaatgtcgg ccagacatcc gagaacttcc
     3781 gggtgcgcga ggtcgccgac atggtggccg aggtcgtgcc cggctccagc gtcacctatg
     3841 aaggcggggc gagcccggac gttcgcaact atcgcgtcaa ctgcgacaag atccggcggg
     3901 tcctgccggc ctttcgcccg gcctggacgc tgcgggccgg catcatccag ctctacgagg
     3961 cctatcgcaa ggtcggcatg accaaggagc aattcctcgg ctcgcgctat ttccgcattg
     4021 gccatgtgaa ggagatgatc gccaagggcg cgctcgacac gcggctcgtg ccgcgatcgt
     4081 ccggcgggtg agggagcgct gccgggcttg cggggtcgcg gcgccgcggc cgttccacgc
     4141 gcaatcgacc attccggtga acagctgcct cctggtcgac gaccggcaga cggcgctcga
     4201 tttcccgacg ggcgatctcg agctcgcctt ctgcgccgcc tgcggcttca tccagaaccg
     4261 gcgcttcgac cccgcagccg tgacctactc caccgattat gaggaaacgc agggcttctc
     4321 ggggcatttc cgccgtttcg tcgacgagct gatcgacgat caggacagca agcaccacct
     4381 ggccggcaag accgtgctgg agatcggctg cggcaagggc gagttcctct cgcgcctggt
     4441 cgagcgcacc ggggcgcgcg gcgtcggcat cgacccgagc tttcgaccgg atcgcctggc
     4501 gggcggggcg accgatcgcc cgaccttcat ccaggactgg tacggcccgg cccatgccga
     4561 tctcgccgcg gacttcgtct gctgccggca cacgctcgag catatcccgg acgtgctgga
     4621 cttcgtccgg ctggtgcggc ggggaatcgg cgaccggcca ggggtccgca tcctgttcga
     4681 ggtgccggac acccggcgcg tgctcgccga gctcgccttc tgggacatct attacgagca
     4741 ctgcagctac ttcaccgccg gctcgctggc gcggctcttc gcccgggcgg gcttcgtcgt
     4801 ggacgatgtg cggacggcct atggcgacca gtatctgctg ctcgaggcgc gcccggccga
     4861 cggttcgagc cggcccggca agtccgtcga tgatcttgcg gagacggcgg cgctggtgga
     4921 gcagttcgca tccggcttgc cgcgcaagct cgaggcgctt cgggcggagg tcgatgcctg
     4981 gcgtggcgcc ggccggaccg tggcgatctg gggctcgggc tcgaaagccg tcgcctatct
     5041 gaccagcctc ggcctggcca ccgagatcgc cgccgtggtc gacatcaacc cgcacaaggc
     5101 cggcaagttc atggccggca cggggcatcg gatcgagcag ccggagcatc tccgggaact
     5161 tcgcccggat gtggtggtcg cgatgaatcc ggtctacctg gccgagatcg gtcaggcgct
     5221 cggcgagctc ggcctggcgc ccgagctgcg cgcgctcgat tgagccgccg gtcatgaccc
     5281 tcgcggccac cctcggctcc cgcttcgcgc tgctgctcgg tggccgggtg gcgctcctcg
     5341 tgctggcgct ggtctcgacg gcgatgctga cccgcatcct cgagcccacc ggcttcggcc
     5401 attaccgcac cgccatggcc tacctggcgc tggtcatcgc gctcgccgac cttggcctct
     5461 ccagcctctt cgtgcgcgag atctcgcgcc ccggcgccga tcagccccgg ctgatcgcca
     5521 acctgctcgg cctgcgcctc gcgttggccg gtgctgcgct cgctttcgcc tctggcctgg
     5581 ccttcctgct gccgctcgac gcgcagggcc ggctcggcat cgtcggtggc tcgttcggct
     5641 ttctcgccta ttcgctgcac ctcctgctct tcgggctctt ccagcagaag ctgcgccagc
     5701 agggggtggt gctcgccgag atcaccggcg ggctcgtgct ggtcgggctg atcatcgtct
     5761 tcgcctgggc cggtgccgag cctttctggt tcgtcgtggc catggggctc agctacatcg
     5821 cgaccctcgc cctgacgttc gtcgcggcgc agcgtctcgt gcgtttcggc ctgcggctgg
     5881 agccggcgct ctggctcgac ctcgcccggc aggcggcccc gctggcggtc gccacgacga
     5941 tcagcgtcct ctatttccgc gcggacaccg tgctgctcgc cttctttcgc gcccccgccg
     6001 aggtcgggct ctatggcgtg ccggtgaagg tgctggattc ctgcatgggc atctcgatgc
     6061 tgctcgtcgg cctcttcacg ccgctgatgg cgcacagcgc gagggtcgac gaggtcgcct
     6121 ttcgtgacca tctgggcacc ggcctcttga ccctcgcggc cggcacgacc cttgtcggcg
     6181 tgggtctcgt ggcactcgcg cccgagatcg tcacgctgct cgctggcgcc gagttcaccg
     6241 gcggtgcctc gatcctgcag gtgctggcgg gtgtgctcgt gctgcatggc acggcggtca
     6301 tcctgcgcga ggcggccacc gccctcacca tccagcagcg gctgctgccc gcctattttg
     6361 ccgggctcgg cgtggcgatg gtcgcctatg tcatgctcat cccgcgcttt ggcggcatcg
     6421 gtgctgcggc ggccctgctg ctcgcggaaa tcgtggtcgt gctgttgacg ggcagcatcg
     6481 tcgccagggc ggcgaccggc ctggcggtca tgcggcgccc gctcatggcc ctcggctgcg
     6541 gcctcgtggc agcggtgctc accgtcgtgg cggagcgttc cggcgtccac tggttcctgc
     6601 gcggattgct cgccggcgtg acctacctga tgcttctgct cggcacccgg accttgcagt
     6661 tgccggcgct gatctcgctc ggccgcgaca tgctcgcccg caggaacgct tgagcaggtt
     6721 ctaggaaaag cctaggaaga tcgcatattt tggagaatga catgaagctg agcgagctgg
     6781 tcgggcacga gctctgggag aagctcctgg ccgatcgcct gggcgagcca ctccatctca
     6841 atgtcgccag cctcttcgtc aagctcttcg gcggctttcg tgccagggtc gccttcgatc
     6901 tgatcgagcg gcgccgctac gcctttccgc tgctcaaggc ggcggactac gccaaagcgc
     6961 agggcatcgg gaagatctac gtgctggagt tcggcatcgc cgccggggcc ggcctcgtca
     7021 atctcgcctg gctcgccgag caggtgaccg ccgaaacggg tgtcgagatc gccgtcgtcg
     7081 gcttcgacgc cggcacgggc atgcccaggc cgaaggacta tcgggactat cccgaggaat
     7141 tcatcgaggg cgacttcccg ctgcccgaca gggaagcgct cgcccgcgtg ctgccgagtg
     7201 gtgttcgcgt cgtctacggg ccggtggccg atacggtggt cgacttcatt gcgaccctcg
     7261 acgcaccggt cggcttcgcc gtgctcgacc tggcctacta ctcgagcacg gtcgaggccc
     7321 tggcggcgct tgccggcccg gcagaacggt atttgtccat gaccctcgtc tatctcgggg
     7381 ccatcaggat cgacaatgcc aacccggcgg tcggcgagct gctggcggtg cgcgagttca
     7441 atgcggcccg gccgctcagg cagatccatc cgttcaccag cctgcgcgac aagcgggtgt
     7501 tccgccgtgc cgcatggctc gaccagatcc atgtgttgca cgtcctcgat cacccgcgcc
     7561 gcgccggcat ggcgccactc gtgcgcgagg cgtggctgct cgaggacccg agccgccagc
     7621 cggcgcgcta gggtacttgg tggcccgaag caaaattgtc tcacctgggc tggccgatac
     7681 cggaggtgcc ttgtggcccc gatttcgcgc ttggcgggag aatgtgacaa tcctatcctg
     7741 ctcgaggcgg cgtcgagtgg aggcccgagt tctttgtgcc ccctcggctg accggacggc
     7801 aatgggcggc gggaccggat tgatgtggct ttcccggaca gtgcttcgga cagtgggatg
     7861 agtggcttcg atggctgagc tgggtccgtt gtgccgcagt tgcgggctcg agctggtcca
     7921 cgaggtcctg gatctcggca tgtcgccgtt gtgcgagagc ttcttgcgcg gtgaccagct
     7981 cgaccgggcg gaagccttct atccgttgaa ggtgctcgcc tgcgctggct gcgggctcgt
     8041 gcagctgcgg gaatacgtga gcccggacga gatcttcggc gagtacgctt acttctcgtc
     8101 gttctcggaa agctgggtgc ggcatgcggc ggagtattgt cgcaccatgc atggccggct
     8161 gggcctgggc ggctcgtcgc tggtggtcga gctcggcagc aatgacggct atctgctcca
     8221 gcatttcggc gagctggggg tgccggtcct gggcatcgag ccggcggcca atgtggcggc
     8281 agcggccgag gccaggggcg tgccgacgcg ggtcgcgttc ttcgggacaa aagtggccgg
     8341 cgagctcttg gccgaggggc gggcggccga tctgatcgtc ggcaacaacg tgctggcgca
     8401 ggtgcccgac ctcaacgact tcgttgccgg catcgctctc ctcctgaagc ccacgggcac
     8461 ggcgaccctg gagttcccgt cgctcgagca gaccatcgcc ggcaaccagt tcgaccaggt
     8521 ctatcacgag catttctcgt atttctcgct gatcgcggtc gaggcgctgg tcgcccggca
     8581 cggcctggtc gtggccgatg tcgaggagct ggcgagccat ggcggctcac tgcgtgtcca
     8641 tctccggcag gcgcgggaag cgtccgggat cgccccccgg gtcgaggcgc tgcgtgcccg
     8701 ggagctggcg gcgggctgcg ccgatccggc gcgcctcgcc gagttcggca gcgtggtcga
     8761 ggccaccaag cgccgcctcc tttccttcct catcgaggcc agggaggcgg gcaagcgggt
     8821 ggtcggctac ggggccccgg gcaagggcaa cacgctgctc aactattgtg gcatccgcac
     8881 cgatttcctc gactatctgg tcgatcgcaa cccctacaag cacggccgct tcacgcccgg
     8941 cacccacatt ccgatcttcc cggtcgagcg gatcgccgag acccggcccg actatcttct
     9001 gatcctgccc tggaacctgc ggcgcgagat cgtccggcag atggcgcccg tcatcgcccc
     9061 ctggggcggc cgcctcgtcg tgccgatccc gaagctggaa gtgatcgagg ccacggaggt
     9121 cgccgcatga aggtcgtgct cttctgcggt ggcctcggca cgcgcatccg cgactactcg
     9181 gaaaacgtgc ccaagcccat gatcccggtc ggccagcagc cgatcctctg gcatgtcatg
     9241 cagtactacg cgcattacgg gcatcgggac ttcgtgctct gcctcggcta caaggccaac
     9301 gtggtcaagg aattcttcct caactaccgg ccgacggcga cccgcgactt catcgtctcg
     9361 aaccacggcc agagcatcga gctgctcggc gagcctgagc cggactggcg cgtggcgctg
     9421 atcgacaccg gcatgtggcg caacgtgggt gcccggctct gggcggtccg caagcacctc
     9481 cagaacgagt cgatgttctc ggccaattac agcgacggcc tgaccgacgt gaacctgccc
     9541 gagatggtcg agttcttcga ggccagcggc aaggtcgcct gcttcctcgc cgttcagccc
     9601 aacttcagct accacctcgt cgatttcgac gagagtggcc atgtggcggc cctgcgcacc
     9661 agcaaccagt cggaaatctg gatcaatggc ggcttcttcc tctttcgccc ggagatcttc
     9721 gactacatgc ggcccggcga ggagctcgtc gtcgagccgt tccgccgcct gatcgaggcc
     9781 gatgagctcg tcgcctaccg ccatcgcggc ttctgggcgt cgatggacac gctcaaggac
     9841 aagcagatgc tcgaggatct cttcgagcgc ggcgagacgc cctggctgcc ctggcttcag
     9901 caggccaagc cggcgtgatc cctcttcgtc tcgggcagtc ggggcagccc ccgccggcgc
     9961 agccccggtc agggcagcca ttgcgtctgc tctgcctcgg cgcccacgcg gacgacatcg
    10021 agatcggctg cggcggcttc gtgctcgacc gccttgcccg ggagcggccg gtccatgtcg
    10081 actggtgcgt gctcagcgcc agccccgagc gggcgaccga ggcgcgcgcc agcgccgccg
    10141 cctttctagc cgacgccgcc tcgtccgagg tcaccgtcgc cggcttccgc gacggctact
    10201 tcccggccga gcgcgttgcc atcaaggact ggttcgaggc gctcaaggcg cgcccgcggc
    10261 ccgatatcgt cctcacccac acgcagaacg atcgtcacca ggatcaccag ctcgtctggc
    10321 agctcacctg gaacaccttc cgtgatcacc tcgttctcga atacgagatc ccgaagtacg
    10381 agggcgatct gtccaccccc aacctctacg tgccgttcac tgaagcggtg ctcgcccgca
    10441 aggtcgagct cctgctcgag cacttccctt cccagcgtcg caagccctgg ttcgacaccg
    10501 agacgtttcg cgggctcgcc cggctgcggg gtctcgaagc cggctcgccg acccgctttg
    10561 ccgaggcgtt ccacgccagg aagctggtcc ttcagtgagc ggcgagcggg cagtggatga
    10621 tcgaccgccg accgtcagca tcggggtgcc ggtctacaac ggcgagaact tcctcggcga
    10681 cgcgatcgaa tcggtgctcg cccagagcgt cggcgacttc gagctgatcc tctgcgacaa
    10741 tgcctcgacc gatggcaccc gggcgatctg cgaagcctac gcggcgcgcg atcggcgcat
    10801 ccgctactac cgcaacccgc gcaatctggg tgcggcagcc aactacaacc gtgccttcat
    10861 gctgaccagg ggccgctact tcaagtggct cgcccacgac gatcggctgc atccggcctt
    10921 cctggagcaa acggtggccg tcatggaagc gcggcccgag gtcgtgctct gcggcagcca
    10981 ggtcgaggtg atcgatgcgc aaggtgcgcc gatcggcacc tacggcagca ttctcagcca
    11041 ggcggacgtg gccagcccgg cggagcgctt cgcggtctac gtgctgcggc cgcacacggg
    11101 cgtcgacatc ttcgcattga tccgccgctc cgccctcgag ggctcgctgc tgcactcgca
    11161 tttccatggc gccgatcggg cattgttggc gcagctcgcc ctgcgcggcg ccatggtcca
    11221 gatccccgcg accctgaacc agatccgcga gcacgatcaa cgctacaccc ggcgggccaa
    11281 gtcggcgcgc ctccgcctgg actggcacgg ctccagcccg ggcggtatcg cggccatacc
    11341 gatcctgcag ctctatcagg cctatcgtcg ggtggtggcc ggcacggcga tcgcgaccga
    11401 ggagcgctgg cgctgtcggg caacgctcgc ccgctggtgg ctcgtcaact ggaacagcat
    11461 gcgggtcgcc gtcgatatcg cggccctcgt cgtgcccgac atcgtcgggt tcgccgaagg
    11521 cgtgaaggaa cggctggtcg gcgcgtcggc gggccacttc aagccgccgc gctgaacctc
    11581 cttgcccggg accggcgctc agcggacgat atcgctgccc ttcatgccga gggccttcgc
    11641 caggtccttg aggcggctgc gcaccggctc gctgtcgggc acgtcctcgt cggtggacag
    11701 cctcagcagg acacgctcat cgacgacctc catgcgcgcc tgcgccagca gctctggaac
    11761 gctgcccgag aaacggtaac ccagtcgcat ggcgcaaccg agcacgcccg cgcgttggcg
    11821 gagtgccggg tcgagcaggg ccgcggcggc cgccagcccg ggctcgccat cattcccgcc
    11881 gtagcgccgg tggacggcga gggcaatgaa cacccgctcc gcatggtcga gcgccacgaa
    11941 cgggaactgg ctgagccggt gaaagctctg ctgcgcgcgc agctccgcat ggtcccgcca
    12001 gccgatatcg gagagctcgc acgcagcgat ccgcagccgc tgctcccgct cggtctcgcc
    12061 cggaaacaag ggggcggtcc aatgcaccat ggcttcgccg atatgtggca cccgcgctcg
    12121 cggccggccg aacgtggcgg cgccgaccag cagcggatcg agctcccgct cgtcggccgg
    12181 caggcaccga tgcagccagc cctcgcgcag cccgagggcg gagaagacga tccgttccgg
    12241 cgccatcgcc ttcagcaggc gatcgaggac gagggccgcc gcgcgcacgg tggcggcgcg
    12301 ccgggaggga accccgggca gcgccgatac ctggtcgggt gcggttctca ggatggatct
    12361 ggccaggctg cgggccgcgt cgcggtcgag ctcgaggccg tgcacgaccc gcaagggtgc
    12421 ctcgaccgtg gcgacatgta tccgcgcgag cgcccgccag ccgccgccga cgacaaagaa
    12481 ccgttccacg tcccgggcga cccaggggcg gttcgcgaag atctcgtcga ggtgcttttt
    12541 ggccgcacgc tcgtcgctcg ccatgagggg caacacgcgc aaggcgccga tcggcaggct
    12601 gatcgccccg ctgccgacgc gaccttcgct gaccgacgag agctcgaggc tgccgccgcc
    12661 gagatcgccg acgatcccgc tcggtcgatg aaagccggcg gcgacgccct gcgccgaaag
    12721 ctgcgcctcc tcgcccccct ccacgatccg gacctcgcga ccggtgcgtg cgcggatcgc
    12781 atccaccagc tcgccggcgt tgtcggcacg ccgaaccgcc tcggtcgcga gaaggtcgat
    12841 gcgctccgcc tgcatggcac gagcgatgac gcaatagcgg tgaacggcgt tgacgcaggc
    12901 gacgatgccc gcctccggca atcgaccggt cgtgtcgagc tcgctgccga ggccgcaaag
    12961 cgatttctcg ttgaagcgcg ggaacggtgc ccggctgagc gtgtcgtaga tgacgagccg
    13021 gaccgagttc gagccgatgt cgacgaccgc gaccggcgca tcgcgggaga tcatccgggc
    13081 gggcgagacg ccggtcatgt catgaccgcc cgacgcgccg cccggtcaat cgccggcatc
    13141 gtcggcatgg ccgggcacca gatgcaggtg ctgcgcccgg ctcttgctct tgcgcaaggc
    13201 gctgccgcgc ccggagaggc tcgggttggt catgaagtag gtatgcgccg cgaacgcctc
    13261 gctgccggag ccgagccggg agtaggtccc gtcggtgttg agcagccagc tctgcgccgt
    13321 gtccttcagg ttggccacca tgacctgcga catgatctgc tggtgcaccg tctcgttctc
    13381 gatcggcacc agcgtctcga cgcggcgcga caggttgcgg gacatccagt cggccgacga
    13441 gatgaacacc ttggcctggc gggacggcag gccgaagccg ttgccgaagc agatgatccg
    13501 tgaatgcgcg aggaagcgcc cgatgatgct ct
//
LOCUS       DAOTFI010000133        13516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5456_length_13516_cov_7.144491, whole genome shotgun sequence.
ACCESSION   DAOTFI010000133 DAOTFI010000000
VERSION     DAOTFI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13516
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5456_length_13516_cov_7.144491"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="cbiB"
                     /locus_tag="P5558_14185"
     CDS             <1..932
                     /gene="cbiB"
                     /locus_tag="P5558_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HRY25510.1"
                     /translation="ALLIDRVAGEPDAVWRRLPHPVVLVGRAIDLLERRLRRPADTPA
                     RERRMGTVLITLLTLAGLACGWFLQTALLALPLGWLWLGLIMSTLIAQKSLLDHVDAV
                     AAGLQQSIEVGRLAVARIVGRDTATLDGPAIGRAATESLAENLSDGVIAPLFWALLLG
                     LPGILAYKIVNTADSMIGHKTPRHLHFGRAAARLDDALNWLPARLTASLVIVAASCTG
                     TAREAWRITRRDARLHRSPNAGWPEAAMAGALGVRLSGPRIYGGKPTIEPWVGDGSAE
                     IGPAKLRQALRLADRVWLLVLVAVLGFCGLSLT"
     gene            complement(919..2382)
                     /locus_tag="P5558_14190"
     CDS             complement(919..2382)
                     /locus_tag="P5558_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRY25511.1"
                     /translation="MSAALMIQGTSSDAGKSLLVAGLCRHFANRGLAVRPFKPQNMSN
                     NAAATADGGEIGRAQALQALACRVSPDVHMNPVLLKPTGELGAQVIVQGRMLSQADAR
                     GYHALKPRLLPAVLESFARQRAEADLVIVEGAGSPAEVNLRQGDIANMGFAVAAEVSV
                     LLVGDIERGGVIASLVGTMALLEPSERALIKGYIVNKFRGDPRLFDSALPLLLERAGL
                     TCLGILPWFAAARDLPAEDVLGLVGTRRPGGIRIAVPRLQRISNFDDLDPLRLEPDVD
                     LVLLEAGEALPGDTDLVLLPGSKATRSDLAALRAAGWDIDILAHHRRGGRVLGLCGGF
                     QMLGRSVHDPEGLEGPPGSSQGLGLLDVETVLAPAKTVRQAKAVDVSSGLEVEGYEIH
                     QGRTEGPDTIRPMLWIEGHPDGAISADGRVMGAYLHGLFVADRFRRSFLGRLRERPAG
                     GEAYAARIDRVLDELADHLATHLDMRRIEAISGVTSG"
     gene            complement(2379..3293)
                     /locus_tag="P5558_14195"
     CDS             complement(2379..3293)
                     /locus_tag="P5558_14195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRY25512.1"
                     /translation="MSNSDHPGLSEHAKGLLIVATGVLILTPDALLLRLIEADPWTLL
                     FWRGIGFFSVQMTVSACRHGGCLWRVIRATGWIGVAIMALHGVTQLLFVYSINHTLVA
                     NTLVIIAASPLAAALLAWLLLGERTPPRTLIAGGVALAAVALTVGGGYATGGLAGDLA
                     ALALMLVMALIFVLLRKAKARNMLPAVALSGLVTAALALPFAPTLAVTGNDLIYLPIL
                     VGLVVPISFILISSGPRYLPAPEVGLLMLVETVLGPLWVWFAFAEEPTGASLVGGGII
                     ILALGIHSWIGLRAERRRRRPPAAVQPA"
     gene            complement(3280..3882)
                     /gene="cobO"
                     /locus_tag="P5558_14200"
     CDS             complement(3280..3882)
                     /gene="cobO"
                     /locus_tag="P5558_14200"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRY25513.1"
                     /translation="MTDSDATHALKKARIKAAKDKLYASKTIEKGLLIVHTGTGKGKS
                     TAAWGLAMRTLGHGLKLGVIQFVKGRRDTGERRLLERFPDLVKIKVMGEGFTWETQDR
                     ARDIAAAEAAFAEAEAMLQDPSLHMVILDELNIVLRYDYLPLARVLGAFRTKRPDLHV
                     VVTGRNAKPELIEMADLVTEMTLVKHPFRAGVKGQLGVEF"
     gene            4014..4991
                     /locus_tag="P5558_14205"
     CDS             4014..4991
                     /locus_tag="P5558_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25514.1"
                     /translation="MLELTTTMLVSCLAASLVLTVLVALAVLARTGLRGRRWLLICGF
                     GLGLVCGAVELGLPRQLPPLTLLVDGWPYAVWLEIGLHALFAGLTYFVIVGILLRAVM
                     QLSRPTARRRGDVVALAAGLGCGLALSATALRLGLIGVWPPSALFVAVVYPPVQLCFA
                     LLLAAGVLATRAGAHMASRFWKLAAAVLQTGYQFVLRTNDTIGHWLAWLEPESIGWLW
                     LGLIGLAWALGLAVMVGLGRAEPPEAHIETASPGGLLSARFWLWAGIVVLAPTGALLA
                     LGLSADLGVLTARVMLLALFATPLMAAAILLRTALSLQRRPTEPSAVPR"
     gene            complement(4945..8643)
                     /gene="cobN"
                     /locus_tag="P5558_14210"
     CDS             complement(4945..8643)
                     /gene="cobN"
                     /locus_tag="P5558_14210"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HRY25515.1"
                     /translation="MHLLSARSAPSDVGDDAVDLGQSPAELVILSAAESELSGLAAAH
                     ARRGAAARPTLRLANLLQLHHPLSVDLYVEKTLCAARLIVVRLLGGRSYWSYGVEQLR
                     RLALERGIGLVCLPGDDRPDPELARLSTLPNELLDALHGYFLHGGIENLGAALEFLAS
                     ELGYDATPPSPRALPKAGLYCEPKGDGRPIAALVFYRAMVQSGDLALIDAIIAALDEA
                     GLNCLALHVAGLKDPEVQAVVEAALARHPVSIVLNLTGFAVTTPGAAASRTPFSATGR
                     PVLQLIASGGSESAWAAGTTGLSPRDIAMSVALPEIDGRLLTRAVSFKGDPVTDAATE
                     ITLTRYRPVPDRVAFVARLSRAWVDLAKTPAAERRVAVLLANYPDRDGRLANGVGLDT
                     PASTLAVLEALAQAGYHVTDAPPDTGALMAMLQAGPTNALADRATRRSTISLPVETYR
                     RLLATIPAAARAEIDARWGPPEVDPHVVDGAFRLSLHSLGRVVLGIQPARGYQVDPNA
                     TYHSPDLVPPHGYLACYWWLRHIWGAQAVVHLGKHGNLEWLPGKALALGATCWPEIAL
                     GPTPHLYPFIVNDPGEGSQAKRRAQAIIIDHLTPPLTRAEHYGELAALEALVDEYASA
                     RELDPRRLAPLRQQILELSARLGLDRDLGLDPRDEASLTTLDNHLCELKELQIRDGLH
                     ILGSAPTGSRRTDLLLALTRLKRGRGEGADASLITALARDLGIGPDPMTVEPTAAAGT
                     VPPLLGRCRHMGDVRAALEDIARSLVGGDLPPGPDWQATSAVLGFIQTELAARLESGA
                     TAELSALLAGLDGRFVEPGPSGAPTRGRLDVLPTGRNFYSVDTRAVPTPTAWTLGWRS
                     AETLIEQHRQEHGDWPRRIALSAWGTANMRTGGDDIAQALAFLGCRPLWEPASGRVTG
                     IELLPLDVLGRPRIDVTMRVSGFFRDAFPEQMALFDDAVRAVAEADEPAAMNPLGEAF
                     RRDRDRLVAEGVDGAAARRQASYRLFGSKPGAYGAGLQALIDSGGWVDESELAESYLA
                     WSSWAYGRGVDGHAASGELRDRLHAIEVVLHNQDNREHDILDSDDYYQFQGGLTVAVR
                     HLAGRQPIVLHADHANPGRLRIRGLKEEIGRVVRGRAANPKWLNGVMRHGYKGAFEIA
                     ATVDYLFAYAATTRLVENHHFEVLFDAYLGDEQVRDFMTENNPAALRETADRFAEAVE
                     RGLWQPRRNSAQAIIEGLRMAPSGDAAD"
     gene            complement(8648..9178)
                     /gene="cobU"
                     /locus_tag="P5558_14215"
     CDS             complement(8648..9178)
                     /gene="cobU"
                     /locus_tag="P5558_14215"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRY25516.1"
                     /translation="MSIFLPRLTLILGGARSGKSRHAERLVLESGLQPCYLATGQGGD
                     AEMAARIAAHRARRGERWRLVEEPLELATALRHHAAADCAVLVDCLTLWVTNLLLAER
                     DIEAEVAGLTAILQALPGPVVLVSNEVGLGIVPIDALTRAFVDATGALHQRLAAMADR
                     VDLVTAGLPLRLKPEG"
     gene            9268..9918
                     /locus_tag="P5558_14220"
     CDS             9268..9918
                     /locus_tag="P5558_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200000.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HRY25517.1"
                     /translation="MDLLQHPDLASQLTALLSVVMIDIVLAGDNAVVVGTAAAGLPPW
                     QQRRVIAIGATLALLARVAFALIAVQLLAIIGLLLAGGLLLLWVAWKMWRELKAGEAA
                     AAGAIPHVLPGGGAVAGAGQKTFRSAIIQVAVADISMSLDNVLAVAGAARDHPWIMIF
                     GLVLSIALMAVAAGFIARLIDRYRWLAYVGLLIIVVVAVKMIWEGGHEVLAVTAFL"
     gene            complement(9922..11496)
                     /gene="pckA"
                     /locus_tag="P5558_14225"
     CDS             complement(9922..11496)
                     /gene="pckA"
                     /locus_tag="P5558_14225"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRY25518.1"
                     /translation="MREVAQDILDAARPGRLHWNLVPARLVTMALEGGEARLSAEGAL
                     VAETGEITGRSADDKYFVDRGELTSGVDWSKANKRLAAADFSALRHTALRHMAGRSLF
                     VQDLWAGADPAARLRVRIVTEQAWHSLFARNMFRRPPDCELHAFAPHWTVLQLPSLRA
                     NPSQHGTRSGTAIVIDLSSRVVLIVGTAYAGEIKKSVFTVLNHLLPASGILPMHCAAN
                     VGAAGDVAIFFGLSGTGKTTLSADPKRQLVGDDEHGWGPHGVFNLEGGCYAKAIRLDA
                     EAEPEIWQASTRFGTILENVVLDPLTAAADFDDATLTENTRSSYPLEFVANAHPTGMA
                     GHPRNIVMLTADAFGVLPPISRLTPEQAIYHFLSGYTARVAGTERGVDTPQATFSACF
                     GAPFMPRHPTVYAELLRRRLAGNDVTCWLVNTGWTGGAAGTGRRMPLEATRALLDAAL
                     DGRLAASRFSEDPAFGLAVPTSCPGVDDRLLSPRAAWSDPTAYDVAAREVAGLFEANF
                     ARFADRADTAGSRAAA"
     gene            complement(11521..12252)
                     /locus_tag="P5558_14230"
     CDS             complement(11521..12252)
                     /locus_tag="P5558_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HRY25519.1"
                     /translation="MTETPALTIRRLVRNTPQAALGTLDAQGAPYVSLVLLAVDHDAS
                     PLLLLSDLADHTKHFRRDPRVALLVDGTAGMASPLAGARATLTGEIERTNAPHHKARF
                     LARHPDAGGYAGFGDFNFYRLNLRRAHLVAGFGRIHWVEGSDVLLPVDDQLPLRAAEE
                     DILAHMNTDHAAAVELYATRLLGRPAGTWRLTGIDPEGTDLRLGGSVARLWFDHPVRD
                     AESARGALVRLARRARRPDEPTGER"
     gene            12471..13469
                     /locus_tag="P5558_14235"
     CDS             12471..13469
                     /locus_tag="P5558_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY25520.1"
                     /translation="MEPIELGRPVEVAPGILWLRLPLPYALDHVNVWLLDDGDGWTLI
                     DTGIGDERSRAIWERLLAEEFMARPIGRIVATHFHPDHIGLAGWLAERLGVPLLTSRT
                     EWLSARLLALDSTSGYAEAGRSYDIAAGLDAGVVEQRFAAGNRYRTSVSMPPAVFTQV
                     AAGDTIEMGGGSWRVLIGEGHSPEMLTFYSAERNLLIAADQVLPRISPVVAVWPTTPG
                     ADPLRDFLATLPQYLELPEDCFVLPSHDAPFQSLHERIRSLVRHHEERLELTRRACAE
                     PATVARIIPTLFRRKFDAHQLGFALGETLAHVNYLLHQGHLAVVGSEGGARLYGLP"
BASE COUNT         2013 a         4552 c         4863 g         2088 t
ORIGIN      
        1 ccgccctgct gatcgaccgt gtcgcgggcg agcccgacgc ggtgtggcgc cgcctgccgc
       61 atccggtcgt gctggtcggg cgggcaatcg accttttgga gcgtcgtttg cggcggcctg
      121 ccgacacacc cgcgcgcgag cgccggatgg gcacggtgct gatcaccctg ctgaccctgg
      181 caggtctggc ctgcggctgg ttcctgcaga cggcacttct ggcgctgccg ctcggctggc
      241 tctggctcgg gctcatcatg agcacgctca tcgcccagaa gagccttctc gatcatgtag
      301 acgccgtcgc ggccgggcta caacagagca tcgaggtggg gcggctcgcc gtggcgcgga
      361 tcgtcggccg cgacacggcg acgctcgacg ggccggcgat cggccgggct gccaccgaat
      421 cgctcgccga gaacctctcc gacggggtca tcgccccgct cttctgggct cttctgctcg
      481 gcctgcccgg catcctcgcc tacaagatcg tcaacacggc agacagcatg atcggacaca
      541 agacccctcg ccaccttcat tttggtcggg cggcggcacg gctcgacgac gcgctgaact
      601 ggctgccggc acggctaacc gcgtcgctcg tcatcgtcgc tgccagctgc acgggcacgg
      661 cgcgggaagc ctggcggatc acgcggcgcg atgcgcgcct gcaccgctcg cccaatgccg
      721 gctggccgga ggccgccatg gccggcgctc tcggggttcg cctctcgggg ccgcggatct
      781 atggcgggaa gccgaccatc gagccctggg ttggcgacgg cagtgcggag atcggcccag
      841 cgaagctccg acaggcgctg cgcctcgctg accgggtctg gctgctcgtc ctggtcgccg
      901 tcctcggttt ctgcggcctc agcctgacgt gacgccagag atagcctcga tccggcgcat
      961 gtcgagatgg gtggcgagat gatccgccag ctcgtcgagc acgcgatcga tgcgcgccgc
     1021 gtaggcctcg ccgccagctg gccgctcccg cagtcggccc aggaaggatc gccggaagcg
     1081 atcggccacg aagagcccgt gcagataggc gcccatcacc cggccgtcgg cgctgatggc
     1141 gccatcgggg tggccctcga tccagagcat cggccggatg gtgtccggac cttcggtgcg
     1201 gccctggtgg atctcgtagc cctcgacctc gagaccgctc gatacgtcca cggcctttgc
     1261 ctggcgcacg gttttggcgg gcgccagcac cgtctcgaca tcgagcaggc cgagcccctg
     1321 gctgctgccc ggcggccctt cgagcccttc aggatcgtgc accgaccggc cgagcatctg
     1381 gaagccgccg cagagtccca ggacccgccc gccgcgccgg tgatgcgcca ggatgtcgat
     1441 gtcccagccg gcggcgcgga gagcggcgag atccgagcgc gtggccttgc tgccggggag
     1501 gaggacgagg tcggtgtcgc cgggcagagc ctcgccggcc tcgagcagga cgaggtcgac
     1561 gtccggctcg agtcgcaggg gatcgagatc gtcgaaattg ctgatccgct gcaggcgggg
     1621 cacggcgatc ctgatgccgc ccggtcgcct ggtgccgacg agcccgagca cgtcctcggc
     1681 cggcaggtcg cgcgcggcgg cgaaccacgg caggatgccg aggcaggtga ggccggctcg
     1741 ctccaggagg agcggcaggg ccgaatcgaa gaggcgagga tcaccccgga acttgttgac
     1801 gatgtagcct tttatcagcg cccgctctga cggttcgagc agcgccatgg tgccgaccag
     1861 cgacgcgatg accccgccgc gctcgatgtc gccaacgagc aggacggaca cctcggcggc
     1921 cacggcgaac cccatgttgg cgatgtcgcc ctggcggaga ttgacctcgg cggggctgcc
     1981 ggcgccctcg acgatgacga gatcagcctc ggcccgctgc cgggcgaagc tctcgagcac
     2041 ggccggcagc aggcggggct tcagcgcgtg gtagccgcgg gcgtcggcct gcgacagcat
     2101 gcggccctga acgatcacct gggcgccgag ctcgcccgtc ggcttgagca gaaccgggtt
     2161 catgtgcacg tcgggggaaa cccggcaggc cagggcctgc agcgcctggg cccggccgat
     2221 ctcgccgccg tcggcggtgg ccgccgcgtt gttgctcatg ttctgcggct tgaagggccg
     2281 gacggccagc ccccgattgg cgaagtggcg gcagaggccg gccacgagca gcgacttgcc
     2341 ggcatcggac gacgtgccct ggatcatcag ggcagcgctc atgcgggctg gaccgccgcc
     2401 ggcgggcgcc gacgccggcg ctcggcccgc aggccgatcc aggagtggat cccgagcgcc
     2461 aggatgatga tgccgccgcc gacgaggctg gcaccggtcg gctcctcggc gaaggcgaac
     2521 cagacccaca agggcccgag gacggtctcg accagcatca aaaggccgac ctcgggggcg
     2581 ggcaggtagc gcggtccgct gctgatcagg atgaacgaga tcggcacgac cagtccaacc
     2641 agaatgggca ggtagatcag gtcgttgccg gtaacggcga gggtcggggc gaagggcagg
     2701 gcgagggcgg cggtcaccag gccggagagt gccacggccg ggagcatatt ccgggccttg
     2761 gccttgcgca ggaggacgaa gatcagcgcc atgacgagca tcagagccaa cgcggcgaga
     2821 tcgccggcga gcccgcccgt ggcgtagccg ccaccgacgg tcagggccac ggcagccagc
     2881 gccacgccgc cagcgatcaa ggtgcggggc ggcgtgcgct cgcccagcag cagccatgcc
     2941 aggagggcgg cagccagtgg cgaggcggcg atgatgacca gcgtgttggc aacgagcgtg
     3001 tggttgatcg agtacacgaa gagcaactgg gtaacgccgt gcagcgccat gatcgcgaca
     3061 ccgatccagc cggtggcgcg aatcacgcgc cagaggcagc cgccgtgccg gcaggccgaa
     3121 accgtcatct ggaccgagaa gaagccgatg ccccgccaga acaggagggt ccagggatcc
     3181 gcctcaatca gccgcagcaa cagcgcatcg ggtgtcagga tcaggacacc ggtggcgacg
     3241 atcagcagtc ccttggcgtg ctcggagagg ccgggatgat cagaattcga caccgagctg
     3301 tcctttgacc ccggcgcgga acgggtgctt gaccagggtc atctcggtga cgagatcggc
     3361 catttcgatc agctccggct tggcgttgcg gccggtgacg acgacatgca ggtccggtcg
     3421 tttggtgcgg aaggccccga gcacccgcgc cagcggcagg tagtcgtagc ggagcacgat
     3481 attgagctcg tcgagaatga ccatgtgcag cgacggatcc tgcaacatgg cctcggcctc
     3541 ggcgaaggcc gcctcggcgg cggcgatgtc gcgggcgcgg tcctgcgtct cccaggtgaa
     3601 gccctcgccc atcaccttga tcttgacgag gtctgggaag cgctcgagta accgccgctc
     3661 gccggtgtct cgccttccct tgacgaactg gatcacgccg agcttcagcc catgccccaa
     3721 cgtgcgcatc gccaggcccc aggcggcggt gctcttgccc ttgccggtcc cggtatggac
     3781 gatcagcaac cctttctcga tcgtcttcga ggcgtagagc ttgtccttgg ccgccttgat
     3841 gcgggccttc ttcagggcgt gggtcgcatc gctgtcggtc atctgtggcc tcctgcagta
     3901 cctgccggca actgctaacg ttaacgggcg caccgcgcca taatgccgat cgggacggtg
     3961 cgaggtggca ccggtgtcgt tgtcgggtca agagtggggc atcgttcgag ggaatgctgg
     4021 agctgaccac caccatgctc gtttcgtgtc tcgctgccag cctcgtgctc accgttctgg
     4081 tggcgcttgc ggtcctcgcc cggacgggac ttcggggtcg ccggtggctg ctgatctgcg
     4141 gcttcggtct tggcctcgtc tgcggggcgg tcgagctcgg cctgccacga caactgccgc
     4201 cgctgacgct cctggtcgac ggctggccgt acgcggtctg gctcgagatc gggctgcatg
     4261 cgctgttcgc tggacttacc tatttcgtga tcgtcggcat cctgctgcgc gccgtcatgc
     4321 agctctcgcg ccccacggcg cgccgccgtg gtgacgtcgt ggcgcttgcc gctggtctcg
     4381 gctgcgggct ggccctcagc gcgacggcgc tcaggctcgg actcattggg gtgtggccgc
     4441 cgagcgctct cttcgtcgcg gttgtctacc cgccggtgca gctctgcttc gccctcctgc
     4501 tggcggccgg cgtgctcgcc acccgcgccg gcgcccacat ggcgtcacgt ttctggaagc
     4561 tcgcggcggc cgtgctgcag accggctacc agttcgtgct gcgcaccaac gacacgatcg
     4621 gccactggct cgcctggctc gagccggaat cgatcggctg gctctggctc ggcctgatcg
     4681 gcctcgcctg ggcgctcggc ctcgccgtca tggtcggtct tggccgtgcc gagccacccg
     4741 aggcgcatat cgagacggcg tcgccgggag ggctgctgag tgcccgattc tggctctggg
     4801 ccgggatcgt ggtgctggcc ccgaccggcg cactgctcgc actcgggctc agcgccgatc
     4861 tcggggtgct taccgcccgc gtcatgctgc tcgccctctt cgccacgccg ctgatggcgg
     4921 cggccatcct gctcaggacc gcgctcagtc tgcagcgtcg cccgacggag ccatccgcag
     4981 tccctcgatg atcgcctggg cgctgttgcg ccggggctgc cagaggccgc gctcgaccgc
     5041 ctcggcgaaa cggtcggccg tctcgcgaag cgctgccggg ttgttctccg tcatgaagtc
     5101 gcgcacctgc tcgtcgccga gataggcgtc gaacagcact tcgaagtggt ggttctcgac
     5161 cagcctcgtg gtggcggcgt aggcgaagag atagtcgacc gtggcggcga tctcaaaggc
     5221 gcctttgtag ccgtggcgca taaccccgtt cagccatttc gggttggccg cccggccgcg
     5281 cacgacccgc ccgatctctt cctttagccc ccgaatccgc agccgcccgg gattggcgtg
     5341 gtcggcatgc agcacgatcg gctggcggcc ggcgaggtgc cgcaccgcca ccgtcaggcc
     5401 cccctggaac tggtagtagt cgtcgctgtc gaggatgtcg tgctcccggt tgtcttggtt
     5461 gtgcaggacg acctcgattg cgtggaggcg atcccgcagc tcgcccgagg ccgcatgacc
     5521 gtcgacgccg cggccgtagg cccatgagct ccaggcgaga taggactcgg cgagctcgct
     5581 ctcatccacc cagccgccgg aatcgatcaa cgcctgcagg ccggcgccgt aagcacccgg
     5641 cttcgagccg aagagccggt agctcgcctg ccggcgtgcg gcggccccgt ccacgccttc
     5701 ggcgacgagg cggtcgcggt cccggcggaa ggcttcgcca agcggattca tcgccgccgg
     5761 ctcgtcggcc tcggccacgg ctctgaccgc atcgtcgaaa agcgccatct gctctgggaa
     5821 cgcatcgcgg aagaacccgg agacgcgcat cgtcacgtcg atgcgtggcc tgcccaggac
     5881 gtcgagcggc aggagctcga tcccggtgac cctgcccgac gccggctccc agagcggccg
     5941 gcagccgagg aaggccaggg cctgggcgat gtcgtcgccg ccggtccgca tgttggcggt
     6001 gccccaggcc gagagggcga tgcgccgggg ccagtcgccg tgctcctgcc ggtgctgctc
     6061 gatcagcgtc tccgccgagc gccagcctag cgtccaggcg gtcggggtcg gcacggcgcg
     6121 cgtgtccacg ctgtagaaat tgcggccggt cggcagaacg tccagcctgc cgcgcgtcgg
     6181 cgcacccgac gggccaggct cgacgaagcg gccatcgagc ccggcgagaa gcgccgacag
     6241 ctccgccgtg gctccggatt cgagacgggc cgccagctcg gtttggatga agcccagaac
     6301 ggcgctggtg gcctgccagt ccggtcccgg cggcagatcg ccgccgacca gcgatcgggc
     6361 gatgtcttcg agcgccgccc tgacgtcacc catatgtctg cagcgaccga ggagcggcgg
     6421 cacggtgccg gcggcggcgg tcggctcgac ggtcatgggg tccgggccga tgccgaggtc
     6481 ccgggcgagg gctgtgatca gcgaggcgtc tgcaccttcc ccgcggccgc gcttgagccg
     6541 ggtcagcgcc agcaggagat cggtccgccg ggagccggtc ggtgccgatc cgaggatgtg
     6601 caggccgtcc cggatctgaa gctccttgag ctcgcagaga tggttgtcga gcgtcgtaag
     6661 cgatgcttcg tctcgcgggt cgagtccgag atcgcggtcg agcccgagcc gtgccgaaag
     6721 ctccaggatc tgctgccgca agggggcgag gcgccgcgga tcgagttcac gggcgctggc
     6781 atactcgtcg accagagctt ccagcgccgc aagctcgccg tagtgctcgg cccgggtaag
     6841 gggcggcgtg aggtggtcga tgatgattgc ctgggcacgg cgcttggcct ggctgccttc
     6901 gcctggatcg ttgacgataa aagggtagag atggggtgtc ggtccgagcg cgatctcggg
     6961 ccagcacgtg gcgcccagcg ccagcgcctt gccgggcagc cattcgagat tgccatgctt
     7021 gccgagatgc acgacggcct gggcacccca gatgtgacgg agccaccagt agcaggcgag
     7081 gtagccatgt ggcggcacga ggtcgggact gtggtaggtc gcgttcggat cgacttggta
     7141 gccgcgcgct ggctggatgc cgaggacgac acggccgaga ctgtgcaggc tcaggcgaaa
     7201 cgcgccatcg accacatgcg ggtccacctc cggagggccc caacgggcgt cgatttccgc
     7261 ccgcgcagcc gccgggatcg tggcgagcag ccgccggtat gtctcaacgg gcaatgagat
     7321 tgtcgagcgc cgcgtggcac ggtcggcgag ggcgttggtc gggcccgcct ggagcatggc
     7381 cattagggca ccggtgtctg gtggcgcatc cgtcacgtgg tagcccgcct gggcgagggc
     7441 ttcgagcacg gccagggtgc tcgccggcgt atcgaggccg actccgttgg cgagccggcc
     7501 atcgcgatcc ggatagttgg cgagcagcac cgccacgcgc cgctctgccg ctggcgtctt
     7561 cgccagatcg acccaggccc gggaaaggcg ggcaacgaag gcgacccgat ccgggaccgg
     7621 gcggtaacgg gtgagcgtga tctcggtcgc cgcatcggtg accgggtcac ccttgaacga
     7681 aacggcacgg gtgagcagcc ggccgtcgat ctccggcagg gccacggaca tggcgatgtc
     7741 gcgcgggctg aggccggtgg tgccggcagc ccaggcggac tcgctgccgc ccgaggcgat
     7801 cagctgcagg accgggcggc cggtcgccga gaagggtgtc cggctggcgg cggcaccagg
     7861 ggtggtgacg gcaaagcccg tgagattgag cacgatcgag accggatggc gggcaagggc
     7921 agcctcgacc accgcctgga cctcggggtc cttaagtccg gcgacgtgca gggccaggca
     7981 attcagcccg gcctcgtcga gggctgcgat aatggcgtcg atgagcgcca gatcaccgct
     8041 ctggaccatg gcccggtaga agacgagggc cgcgattggc cggccatcgc ccttgggctc
     8101 gcagtatagc ccggccttcg ggagtgctcg tggcgatggg ggggtcgcat catagccgag
     8161 ctcgctggca aggaattcga gggcggcccc aagattctcg atgccgccat gcaggaaata
     8221 cccgtgcagg gcgtcgagca gctcgttcgg cagggtcgag agccgcgcga gctccgggtc
     8281 gggccggtcg tcgccgggca ggcagacgag gccgatgccg cgctccaggg cgagccgcct
     8341 gagctgctcg acgccatagc tccagtagga acggccgccg agcaggcgaa cgacgatcag
     8401 gcgtgcagca cagagcgtct tctcgacata gagatcgacc gacaggggat ggtggagctg
     8461 caggagattg gccaggcgca gcgtcggccg ggcagccgcg ccgcgtctgg catgggccgc
     8521 ggcgaggccg gaaagctcgc tctcggcggc ggagaggatc accagctccg ccgggctctg
     8581 gccgaggtcg acggcatcat cgccgacatc gctcggggcc gacctggcgc tcaggaggtg
     8641 cacggtctca gccttccggc ttgagacgca gcggcaggcc ggccgtgacg aggtcgaccc
     8701 ggtcggccat tgccgcgagc cgctgatgca gcgccccagt cgcatcgacg aaggcccgcg
     8761 tcagcgcgtc gatcggcacg atgccgagtc cgacctcgtt cgacacgagc acgacaggcc
     8821 cggggagcgc ctgcaggatc gcggtcagcc cggcgacctc ggcttcgatg tcgcgctccg
     8881 ccagcagcag gttggtgacc cagagggtca ggcaatcgac cagcacggcg caatcggctg
     8941 cggcatgatg cctaagggcg gtagcgagct ccagaggctc ctcgacgagg cgccagcgct
     9001 cgccgcggcg tgcgcgatgt gccgcaatgc gggccgccat ttcggcgtca ccgccctggc
     9061 cggtggcgag atagcagggc tggaggccac tttcgagcac cagcctctcg gcgtggcggc
     9121 tcttgccgga gcgggcacca ccgaggatca gcgtcaggcg ggggaggaat atggacatga
     9181 tcggcgcggc atgacgccac gcggtcgacg cgtcaagcgg cgtggcttgt cgcgattgcg
     9241 ccgatctggc agaggctcct cgacatgatg gacctcctgc agcatccgga tctcgcgagc
     9301 cagctgacgg cgctcctctc cgtggtgatg atcgacatcg tgctggccgg cgacaatgcg
     9361 gtggtggtcg gcaccgccgc ggccgggctg ccgccctggc agcagcgtcg ggtcatcgcc
     9421 atcggcgcca ccctggcact cctcgctcgt gtcgcttttg cactgatcgc ggtgcagttg
     9481 ctcgcgatca tcggcctgct cctggccggc gggctgctcc tgctttgggt cgcctggaag
     9541 atgtggcgcg agttgaaggc cggcgaggcc gcggcggcag gcgccatccc gcacgtcctg
     9601 ccgggcggcg gggccgtcgc cggcgccgga cagaagacct tccgttcggc gatcatccag
     9661 gtggctgtcg ccgacatctc gatgtcgctg gacaatgtcc tggccgttgc cggggcggcg
     9721 cgcgaccacc cctggatcat gatcttcggc ctcgttctct cgatcgcgtt gatggcggtg
     9781 gccgccggct tcatcgccag gctgatcgat cgctaccgct ggctcgccta tgtcggcctc
     9841 ctgatcatcg tcgtggtggc ggtgaaaatg atctgggaag gcggccacga ggtcctggcc
     9901 gtgaccgcct tcctctagca gtcaggcggc ggcacggctg ccggcggtat cggcccggtc
     9961 cgcgaaccgc gcgaagttgg cctcgaagag gccggccact tcccgcgcgg ccacgtcgta
    10021 ggcggtcggg tccgaccagg cggcccgcgg cgagagaaga cggtcgtcga cgccggggca
    10081 gctcgtcggg acggcaaggc cgaaggccgg atcctcgctg aagcggctgg ccgcgagccg
    10141 gccatcgagc gccgcatcga gcagggcgcg cgtggcctcg agcggcatgc gtcgcccggt
    10201 gccggcggcc ccgccagtcc agccggtgtt gaccagccag caggtgacat cgttcccggc
    10261 gaggcgccgg cggagcagct cggcatagac ggtgggatgg cggggcatga agggagcacc
    10321 gaagcaggcg gagaaggttg cctgcggcgt gtccacgccg cgctccgtgc cggcgacgcg
    10381 cgccgtgtag cccgagagga aatggtagat agcctgctcg ggcgtcaacc ggctgatcgg
    10441 cggcagcacg ccgaaggcat cggccgtcag catgacgatg ttgcgcgggt ggccggccat
    10501 gccggtggga tgggcgtttg cgacgaattc cagtgggtag gagctgcggg tgttctccgt
    10561 cagtgtcgca tcgtcgaaat cggcggcagc ggtaagcggg tcgagcacga cattctcgag
    10621 aatggtgccg aaccgggtcg aggcctgcca gatctccggc tccgcctcgg cgtcgagacg
    10681 gatcgccttg gcgtagcagc cgccctccag gttgaagacg ccatgtggcc cccagccatg
    10741 ctcgtcgtcg ccgacgagct gccgtttcgg atcggcggag agggtggtct tgccggtgcc
    10801 ggaaaggccg aagaagatgg cgacgtcgcc cgcggcgccg acattggcgg cgcaatgcat
    10861 ggggaggatg ccgctcgccg gcagcaggtg gttgagcacc gtgaagaccg atttcttgat
    10921 ctcgccggca taggccgtgc cgacgatcag gacgacacgg ctggagagat cgatgacgat
    10981 ggcagtgccg ctccgggttc cgtgctggct cgggttcgcc cgcaggctcg gcagctgcag
    11041 cacggtccag tggggcgcga acgcatgcag ctcgcagtcc ggcggacggc ggaacatgtt
    11101 gcgggcgaag aggctgtgcc aggcctgctc cgtgacgatg cgcaccctca gccgcgcggc
    11161 cgggtcggcg ccggcccaga ggtcctggac gaacaggctg cgcccggcca tgtgccggag
    11221 tgcggtgtgc cgaagcgcgc tgaagtccgc cgccgcgaga cgcttgttcg ccttgctcca
    11281 gtcgacaccc gaagtgagct cgccgcggtc gacgaagtac ttgtcgtccg cggaacggcc
    11341 ggtgatctcc ccagtctcgg cgaccagcgc accctcggcg gagagcctcg cctcaccccc
    11401 ctcgagcgcc atggtgacca accgtgccgg aacgaggttc cagtgcaacc ggccaggcct
    11461 ggccgcatcg agtatgtcct gggcaacttc ccgcatgctc ggctccttca tggtcgacat
    11521 ctatcgttca ccggtcggtt cgtcgggccg gcgggcgcgc cgggcgagac ggacgagggc
    11581 ccctcgagcg ctttcggcgt cacgcactgg atggtcgaac caaagtcggg cgaccgaccc
    11641 gccgagccgc aggtccgttc cttcggggtc gatgccggtc agccgccacg tgcccgcggg
    11701 ccggccaagc agccgcgttg cgtagagctc gaccgctgcg gcgtgatcgg tattcatgtg
    11761 ggccaggata tcctcctctg ccgcccggag cgggagttgg tcgtctaccg gcagcagcac
    11821 gtcggaaccc tccacccagt ggatccggcc gaacccggcg accagatgcg cgcgtcgcag
    11881 gttcaggcgg tagaagttga aatcaccgaa gccggcatag ccgccggcgt ccgggtgccg
    11941 ggcgaggaaa cgggctttgt gatgaggtgc gttcgtcctc tcgatctcgc ccgtcaacgt
    12001 cgctcgtgct ccggccaacg ggctcgccat gccggcggtt ccgtcgacaa gcagcgcgac
    12061 acgcggatca cgcctgaaat gcttcgtatg atcggcgagg tcggagagca gcagaagcgg
    12121 gcttgcgtcg tgatcgaccg cgagcaggac gagcgagaca tagggcgcgc cctgggcatc
    12181 gagcgtgccg agagccgcct gcggggtgtt gcggacgagg cggcggatcg tcagggcagg
    12241 cgtctcggtc atggtggtga aagctccggc aagcatgctg cggcgcaaag tgttaagcgg
    12301 cgtgtaccat caaatagtac gccctccggc gtcgggccgg cacattcgcg gcacactgcg
    12361 atcgaccggg actattgtca cgaatgctgc gacgcggcag aactgcctct caaccccgag
    12421 gcagagagca cgaattgtcc agcgagccac ccgccacccg ccaatccccg atggagccga
    12481 tcgagctcgg ccggccagtc gaggtggcgc cgggcatcct ctggctccgc ctgccgctgc
    12541 cctacgcgct cgaccatgtg aacgtctggc tgctcgacga tggcgacggc tggacgctca
    12601 tcgataccgg gatcggcgat gagcgctcac gggcgatctg ggagcgcctg ctggcggagg
    12661 agttcatggc ccgccccatc gggcggatcg tcgccaccca ttttcacccg gaccatatcg
    12721 gcctcgccgg ctggctggcc gagcggctag gggtgccctt gctgacgagc cggaccgagt
    12781 ggctttctgc gcgtctcctc gccctcgact cgaccagcgg ctacgccgag gcggggcggt
    12841 cctacgacat cgcggccggg ctcgacgccg gcgtcgtcga gcaacgcttc gccgccggca
    12901 accgctatcg cacgagcgtc agcatgcccc ccgccgtctt cacgcaggtc gccgccggcg
    12961 acacgatcga gatgggcggc gggtcgtggc gcgtgctgat cggcgagggc cacagcccgg
    13021 agatgctcac cttctacagc gccgagcgca atcttttgat cgccgccgac caggtgctgc
    13081 cgcggatctc gccagtggtc gcggtctggc cgaccacgcc gggcgccgac cccttgcgcg
    13141 acttcctcgc caccctgccg caatatctcg agctgcccga ggactgcttc gtgctgccct
    13201 cccacgatgc gccgttccag agcctgcacg agcggatccg gagcctcgtc cgccatcacg
    13261 aggagcggct ggagctgacg cgccgggcct gcgccgagcc cgcgaccgtg gcccgcatca
    13321 tcccgacgct cttccgccgc aagttcgatg cgcaccagct cggcttcgcg ctcggcgaga
    13381 ccctggccca cgtcaactac ctcctgcacc agggccacct cgcggtcgtc ggcagcgaag
    13441 gcggggcgag gctatacggg ctgccctgag gctgccgggg tttacagtgg cgagaaggcg
    13501 ggtctagtgt tctccg
//
LOCUS       DAOTFI010000134        13409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5512_length_13409_cov_6.640332, whole genome shotgun sequence.
ACCESSION   DAOTFI010000134 DAOTFI010000000
VERSION     DAOTFI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13409
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5512_length_13409_cov_6.640332"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1420
                     /locus_tag="P5558_14240"
     CDS             83..1420
                     /locus_tag="P5558_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRY25521.1"
                     /translation="MSGSAYRESFRLAWPLVLSNISVPLLGMVDTAVVGRLPEPYALG
                     GVALGANLASTLYFTFGFLRMGTTALTAQAFGAGNALELRACLWRAFLLAAAIGLAIA
                     LVMPLLVDAGRLIFAPPELIDPEFTAYARVRLLGAPLALANAVVLGWLLGMQDARAPL
                     ALLIATNLLNGLLDVAFVWGLGFGAGGVAAATVIAEAFGLALGIFLIHLHLARLHAAP
                     RPSRSAVLAPAAFRRLFRLNGDLFVRTVVLQLVFILFMALGSRQGPVILAINAILLNF
                     FLLASYGLDGFAYAAEAMVARAVGARDPAALRAAVRAGLANAATLAIATGLAFWLASP
                     WIVKILTDIPAVRDGAIGYRPFVALLPLVAVWAFLLDGIFIGATRTRLLRNAMILAVL
                     IFAGAALALVPVFGNPGLWTAMLVFMAARGGLLALIYRRAGSGADFASGEAAG"
     gene            complement(1366..2211)
                     /locus_tag="P5558_14245"
     CDS             complement(1366..2211)
                     /locus_tag="P5558_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRY25522.1"
                     /translation="MDLTLDQALALKSGLVLAAFLAFAFAERWRPAVASPLLLRLGHA
                     TKAAWRRLARNLGLFGLNVLIAPLIVLPITWWAADLSLGLRPAFLAGWTGLLVDILVL
                     DLWIYWWHRANHELPLLWRFHQVHHLDELLDTTSAVRFHFGEVVLSALARALVIILLD
                     LSLLAVIVFEALILTGAIFHHSAARIPARLERALASVIVTPSIHWVHHHAIRADTDSN
                     YGTIFSFWDRLFRSRSATARFAGMKIGVERMRDRPLLRLLAAPFGAQPAASPEAKSAP
                     EPARR"
     gene            2401..2883
                     /locus_tag="P5558_14250"
     CDS             2401..2883
                     /locus_tag="P5558_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRY25523.1"
                     /translation="MRSFDLSPLFRSSVGFDQLDKLFDTAFREAARDTSYPPYNIAKL
                     GPDAYRITMAVAGFGHGDLDLTVQDNVLIVKGQIARPEKEVEYLHRGVAQRAFEHRFQ
                     LADHIKVVGADLNNGLLDIHLEREVPEQMKPRKVVIGDQPRGRVIDADEARRKSAAGG
                     "
     gene            2891..3694
                     /locus_tag="P5558_14255"
     CDS             2891..3694
                     /locus_tag="P5558_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRY25524.1"
                     /translation="MSSAPLPPIPPLIGVSACRKVTEMATAHSVGDKYVAAVIDGAGG
                     LPVLIPALGRALPVDTLIDRLDGVFLTGSPSNLDPALYGGPPRPPGDPADCDRDATTL
                     PLIRACLEQGVPLLAVCRGMQEMNVALGGTLFGRLHEVAGRRDHRSDKTLSYEGRYGP
                     RHAVRLRPGGKLQAILGEVEEIEVNSLHGQGIDRLAPRLEVEATAPDGTIEAVSVPSA
                     KTFALAVQWHPEWRVAEIAQHRLLFAAFGAACRQRLNARAAHERPRDMA"
     gene            3669..5009
                     /locus_tag="P5558_14260"
     CDS             3669..5009
                     /locus_tag="P5558_14260"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRY25525.1"
                     /translation="MSDLETWLKEHRITEVECIVADINGIARGKILPGYKFLRSMQDD
                     GLRLPESIFIQTVTGDYPEEEVIDPVIRDIRLRPDPPTIRLVPWYQEPTAQVIADGVH
                     HDGGAVAIAARQVLKRVLALYAESGWRPVVAPELEFFLTKINTDPDYPLEPPIGRSKR
                     PETGRQSYGIEAANEFDPVFEDVYDWCEAQSIDIDTLAHEAGAAQMEINFNHGDPLAL
                     ADQAFLFKRTLRQAALKHGIYATFMAKPMQNEPGSSMHIHQSVYSIETGENLFAGTEA
                     PYSELFLNHIAGLQRFLPAAMPLFAPNVNSYRRLRRHSDAPINVQWGWENRTVGLRVP
                     ESGPKARRVENRVAGADANPYLAIAASLACGWLGMSEELQPTEQIKGSAYRLAQTLPQ
                     QLPDGLKKLNSSPQLKEVLGEAFVRVLIAVKQAEHEAYQSVISSWEREHLLLNV"
     gene            5043..6410
                     /locus_tag="P5558_14265"
     CDS             5043..6410
                     /locus_tag="P5558_14265"
                     /inference="COORDINATES: protein motif:HMM:NF005682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRY25526.1"
                     /translation="MDARADTDRLRALDRDHHLHPFTVFPELRAKGSRIITHAEGCYI
                     FDSNGNRILDGMAGLWCVNLGYGRERLARVAYEQMQELPFYNAFFQTATVPSIELARA
                     LAEILPEGFERVFFASSGSEANDTVIKSVWYYWNLMGKPAKKTFISRTLGYHGVGLGS
                     ASLTGMRFMHDPFDLPLERFHHIGNPYWFAEGGDLDPEAFGKVAAGWLEAKILEIGPE
                     NVAAFVAEPIQGAGGVIIPPSTYWAEVQRICRQYDVLLVADEVICGFGRTGNWWGSTT
                     FDIAPDIIPMAKGLSSGYQPIAAVAFHDRIADVLAASGTEYAHGVTYHGHPVAAAVAL
                     ETIRIMREEGLDTRASGPIGACFRDRLMTLADHSLVGEVRTCGLLACVELVEDKAARR
                     PYPAARKVGLTCREHCFDNGLVMRAIRDGMVLSPPLIVSEREIDEMVTKAADAFDLTL
                     RSLAG"
     gene            complement(6414..7211)
                     /locus_tag="P5558_14270"
     CDS             complement(6414..7211)
                     /locus_tag="P5558_14270"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRY25527.1"
                     /translation="MDLLNLLVLAVVQGITEFLPISSSAHLILVPVLFGWPDQGLAID
                     IAVHVGTLAAVLVYFHRDVLDLVAGLWLAIRRRPDHRSRLVLHLAVATLPIVVAGLLA
                     KDLVGTTFRSGLLIAVTTIVFGIVLWLADRGAEHHDRSLAQMTLRDAIVIGLAQVLAI
                     LPGTSRSGITMTAGLFLRFSRTESARFSMLLAIPTIAAAGLLATLDLLQSGDATLQAD
                     ALWAALLAFVAALCAIWGLMRWLQTSSFTPFVIYRLVLGAFLLFWLL"
     gene            7421..8737
                     /gene="mgtE"
                     /locus_tag="P5558_14275"
     CDS             7421..8737
                     /gene="mgtE"
                     /locus_tag="P5558_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRY25528.1"
                     /translation="MHDDPIGGEHLAPVSFSAENHPADLAELLEEMAPEAARVALLAL
                     PLGHGAETFGYLPPEIQVALARVLSRGELAAIVTEMNGDDRADLYNRLTPEQQEALLP
                     GLAQAEREDIRRLAAHQEGTAGAIMTSDYATLAPGLTVLQAIGKLRLEAPDKETIYRT
                     YVVDAERRLIGSVRLQTLILAQPQRTVEEVMERTTRAVGVEDDQEEVARSIAKYDIIA
                     LPVVDGEGRLVGIVTHDDALDVLQAEATEDFQKVGSVGSMADSIASASIALLYRSRIY
                     WLVLLVFGNLLSGAGLAFFEDTIAAYIVLVFFLPVLIGSGGNAGSQSSTLMVRALATG
                     DVEMKDWGRMFFRELGVAILLGMTMAVAISTIAGFRGGHQVALVVAVSMLLIVTAGSL
                     IGMSMPFILSRFRLDPATASAPLIASIADIMGVVIYFSIARAILLH"
     gene            complement(8701..10713)
                     /locus_tag="P5558_14280"
     CDS             complement(8701..10713)
                     /locus_tag="P5558_14280"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRY25529.1"
                     /translation="MRLGIDTGGTCTDAVLLDEAEGVVASAKALTSHHDLYQGIAAAV
                     RAVLAAAPHEARERIGLVGLSTTLATNALVEGQGGRAGLIMVGFGPESLARGGLGQAL
                     GRAPVAFVGGGHDASAQPRQPLDLAALDAAIGKMAPTVDAFAVTATFAVRNPAHEQVA
                     AERIMALTGRPVTVSSELTARLDAPRRALTTFLNARLVPRIGRLIAAVERLLAETGIA
                     APLMVVRGDGALMAAEVARRRPVETLLSGPAASVVGAAHLSGLRHAVISDVGGTTTDV
                     ALLDDGQPRLAPEGALVGGFRTMVEAVELQTSGLGGDSETRVDEAGRLGLGPRRLVPL
                     ALLADQHPQVLEALHAQAARAATRATDARFALRQDADVALSGLSRSQHRLLDLLAGGP
                     CPLELVIDREHLAVPLKSLALQGIVRLAGFTPSDAGHVLGRQSGWSIEAARLGAQLEA
                     RKRGGGDTVERFAAAVLEAARTASARAVVGAVWEATGGTVGRLAGFLDEPPVAMALAG
                     DEPRAPLAVRFTIDRPLVAVGGPAAVFYEGVAERLGSRLVLPAGHAVCNAIGAVVGEV
                     ARRVELVATRTGDDQLALHLGEAGTLVTDPASARTLLISHAEEAAIAAARSAGAQDPV
                     VSHVCEERRVVLDGGLELLLEIRVRATARGRPSASQWSRIALAIEK"
     gene            10874..12175
                     /locus_tag="P5558_14285"
     CDS             10874..12175
                     /locus_tag="P5558_14285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY25530.1"
                     /translation="MLFTIRSNWALLLGIGLLMLGHGLQNTLLGVRASIEGFPTSVTG
                     IVMSGYSIGFLVSAIVTPRLVAHVGHVRVFAAFTALASSAILAHALLLEPISWFVLRF
                     LTGVCMSGAYVVAESWLNRAATNEDRGSLLSVYMLVQLTAWAGGQLLLNAADPGGFRL
                     FIGVSVLLSIAVVPMLLTASPAPATAAARSFGLIRLYRSSPLGFVGMFGVGLSQGAFF
                     AMGAVFAGKIGLDVAGISLFMALAIVGGVLLQWPVGRLSDRLDRRRVVTGTTILAGLA
                     TGIVVIAGTGTFPVVAAAFFLYGGMCLPMYALCIAHTNDNLDAEDMVGASGTLVLVVG
                     IGMVLGPPLAAAAMDLQGPVGFASFFTIVHLGIGAFALYRMTRRASVPMADQGQHIYA
                     PQPSPVAAALVQETAIGQQEAKEQTEIVQSPLASPAGQGYN"
     gene            12404..12772
                     /locus_tag="P5558_14290"
     CDS             12404..12772
                     /locus_tag="P5558_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY25531.1"
                     /translation="MSSKIFVANFPFSTTSEDLQDLFGQHGEVVSAKVATDRETGRSR
                     GFGFVEMATPAAAGAAIQQLNGYDMGGRPLAVRVAEDRGGAGGGGFGGRGGPGGGRGP
                     GGPGGGPRRDNLGRPPRRPY"
     gene            12917..>13409
                     /gene="hrcA"
                     /locus_tag="P5558_14295"
     CDS             12917..>13409
                     /gene="hrcA"
                     /locus_tag="P5558_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249251.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRY25532.1"
                     /translation="MIVELNARSREVFRQIVDAYMATGEPVGSRTVSRRLDDQLSPAT
                     VRNVMADLEEAGLLCAPHTSAGRLPTDAGLRLYVDGLLELGGLSKDDRQRIETQCAVA
                     GRSIEAVLEDATETLSGLSRYAGLVIAPTTRRPYKHIEFVPLSPGRALVVTVSDGGMV
                     ENRV"
BASE COUNT         2008 a         4732 c         4534 g         2135 t
ORIGIN      
        1 tcggaaagcc caggaagacc agaaggatga agataccgag catcgtcagg gcgacgtcgc
       61 gactgtggca gggaggcctt gcttgagcgg tagcgcctat cgggaatcgt ttcggctggc
      121 ctggccgctc gtcctctcga acatctcggt gccgctgctc ggcatggtcg acacggccgt
      181 ggtcggccgg ctgcccgagc cttatgcgct gggtggcgtg gcgctcggcg ccaatctcgc
      241 ctcgaccctc tatttcacct tcggcttcct gcggatgggc acgacggcgc tcaccgccca
      301 ggcctttggc gccggcaacg cgctggagct gcgcgcctgt ctctggcgcg ccttcctgct
      361 ggccgccgcc atcggcctcg ccatcgccct ggtgatgccg ctcctggtcg atgccggccg
      421 gctgatcttc gccccgcccg aactgatcga tccggaattc accgcctatg cccgcgtccg
      481 cctgctcggc gcaccgctcg ccctcgccaa tgccgtggtg ctcggctggc tgctcggcat
      541 gcaggacgcc cgggctcccc tggccctctt gatcgccacc aacctgctga acggcctgct
      601 cgatgtcgcg ttcgtctggg gcctcggctt cggtgccggc ggcgtggccg ccgccacggt
      661 gatcgccgag gctttcgggc tggcgctcgg catcttcctg atccacctgc atctggcgcg
      721 cctgcacgcc gccccccggc cgtcccgctc ggctgtgctg gcccccgccg cgttccgcag
      781 gctgttccgg ctgaacggcg atctcttcgt gcgaaccgtc gtgctgcagc tcgtcttcat
      841 cctcttcatg gcgctgggct ccaggcaggg gccggtgatc ctcgccatca acgccatcct
      901 tctcaatttc ttcctgctcg cctcctatgg cctggatggc ttcgcctacg ccgccgaggc
      961 catggtcgcc cgggcggtgg gggcgaggga cccggcggct ctccgggccg ccgtgcgcgc
     1021 cgggctcgcc aatgcggcga cgctcgccat cgccaccggc ctcgccttct ggctcgccag
     1081 cccctggatc gtgaagatcc tcaccgacat cccggccgtc cgcgacggcg ccatcggtta
     1141 ccgcccgttc gtggcactcc tgccgctcgt ggcggtctgg gcctttctcc tcgacggcat
     1201 cttcatcggc gccacgcgca cgcgccttct gcgcaacgcc atgattctgg cggtgctgat
     1261 cttcgccggg gcagcgctgg ccctggtccc cgtcttcggc aatcccggcc tctggaccgc
     1321 catgctggtc ttcatggccg cgcgcggcgg tctgctcgcc ctgatctacc ggcgggccgg
     1381 ctcgggcgcc gacttcgctt ctggtgaggc ggcgggctga gcgccgaagg gtgcggcgag
     1441 gaggcggagc agcggccggt cccgcatccg ctcgacaccg atcttcatcc cggcaaatcg
     1501 cgccgtcgcg ctccttgatc ggaacaatct gtcccagaag ctgaagatcg tcccgtaatt
     1561 gctgtcggtg tcggcacgaa tcgcgtggtg atgcacccag tggatcgaag gcgtgacgat
     1621 cacgctggcg agcgcccgct cgagcctggc cgggatgcgg gcagccgaat ggtggaagat
     1681 cgcgccggtc aggatcagcg cctcgaagac gatgacggcc aacagcgaga gatcgaggag
     1741 gatgatcacc agcgctcggg ccagggccga aagcaccacc tcgccgaaat ggaagcggac
     1801 ggcgctggtg gtgtccagga gctcatccag gtggtgcacc tggtgaaagc gccagagcag
     1861 cggcaactca tggttcgccc ggtgccacca gtagatccag agatcgagga ccaggatgtc
     1921 gacgaggagc cccgtccagc cggcgaggaa ggcggggcgc aggccgagcg aaaggtcggc
     1981 cgcccaccag gtgatcggca ggacgatcag cggggcgatc agcacgttga gcccgaagag
     2041 accgagattg cgggcgaggc gccgccaggc cgctttggtg gcatggccga gccgcagcag
     2101 cagcgggctc gcaaccgccg gtcgccagcg ctcggcgaag gcgaaggcga ggaacgccgc
     2161 gagcacgagc ccgctcttga gcgccagcgc ctgatcgagg gtgagatcca tgaccttgca
     2221 tatgtgcggt ccggcagccg ccgtccagcg cggcatggtc acttgaaggt atgggccaga
     2281 cagcccacgt cttgcctgcg gtcgccgctc gtggggccgc atgccgatac cgcccggttg
     2341 ccaggcaacg ggcaaggaac gtctggttgc tccccctttg cgcacttcgg aggactggtc
     2401 atgcgcagct tcgatctttc gccgctcttt cgttcgtccg tgggcttcga ccagctcgac
     2461 aagctgttcg atacggcctt tcgcgaggcc gcccgcgaca cctcctatcc gccctacaac
     2521 atcgccaagc tgggtccgga cgcctaccgc atcaccatgg cggtggcggg cttcggacat
     2581 ggcgatctcg atctgacggt ccaggacaac gtgctgatcg tgaagggtca gatcgcgcgg
     2641 cccgagaagg aggtcgagta tctccatcgc ggggtcgccc agcgggcctt cgagcaccgt
     2701 tttcagctcg ccgaccacat caaggtggtg ggcgccgacc tcaacaacgg cctgctcgac
     2761 atccatctcg agcgcgaggt gccggagcag atgaagccgc gcaaggtggt gatcggcgac
     2821 cagccgcggg gcagggtgat cgatgccgac gaagcgcggc gcaagagcgc cgctggcggc
     2881 tgattgcctg gtgtcgtctg caccactgcc gccgatcccg cctttgatcg gcgtctccgc
     2941 ctgtcggaag gtcaccgaga tggcgaccgc gcacagcgtc ggcgacaagt atgtggcggc
     3001 ggtgatcgac ggggctggcg gcctcccggt gctgattcca gctctgggcc gggcgctgcc
     3061 tgtcgacacg ctgatcgatc gcctcgatgg cgtgttcctg accggcagcc cctccaatct
     3121 cgatccggcc ttgtatggtg gtccgccgcg gccaccgggc gatcctgccg attgcgaccg
     3181 ggatgcgaca accctgccgc tgatccgcgc ctgcctggag cagggcgtgc cgctcctggc
     3241 cgtctgccgg ggcatgcagg agatgaacgt cgcactgggc ggcacgctct ttggccgatt
     3301 gcacgaggtg gcggggcgcc gtgatcaccg ctccgacaag accctctcct atgaaggccg
     3361 ctacggcccg cgacatgctg tccgcctgcg gcccggtggc aagctccagg cgattctcgg
     3421 cgaggtcgag gagatcgagg tcaattcctt gcacggacag ggaattgacc ggctcgcccc
     3481 ccgcctcgag gtcgaggcga cggcgccgga tggcacgatc gaggcggtga gcgttccgtc
     3541 tgccaagacg ttcgcgcttg cggtacagtg gcacccggaa tggcgggtcg ccgagattgc
     3601 ccagcatcgg ctgctcttcg ccgccttcgg cgcggcctgc cggcaacgac tcaatgcgag
     3661 agcagcccat gagcgacctc gagacatggc ttaaagagca ccgcatcacc gaggtcgagt
     3721 gcatcgtcgc cgacatcaac ggcatcgccc ggggcaagat cctgcccggc tacaagttcc
     3781 tccgctcgat gcaggacgac ggcctgcgcc tgcccgagag catcttcatc cagacggtca
     3841 ccggcgacta tccggaagag gaggtgatcg acccggtgat ccgggatatt cgcctgcgcc
     3901 ccgacccgcc tacgatccgg ctggtgccct ggtaccagga gccgaccgcc caggtgatcg
     3961 ccgacggcgt ccaccatgac gggggggccg tggcgatcgc cgcccggcag gtgctgaagc
     4021 gggtgcttgc gctctatgcg gagagcgggt ggcgtcccgt ggtggcgccc gagctcgaat
     4081 tcttcctgac caagatcaac accgacccgg actatcccct cgagccgccg atcggccgct
     4141 ccaagcggcc ggagaccggg cgccagtcct acggcatcga ggcggccaac gagttcgacc
     4201 cggtgttcga ggacgtctac gactggtgcg aggcccagtc gatcgacatc gacaccctgg
     4261 cgcacgaggc cggtgcggcg cagatggaga tcaacttcaa ccacggcgac cctttggccc
     4321 tcgccgacca ggccttcctc ttcaagcgga cgctcaggca ggctgccctc aagcacggca
     4381 tctatgccac cttcatggcg aagcccatgc agaacgagcc cggcagctcg atgcacatcc
     4441 accagagcgt ctactcgatc gagacgggcg agaacctttt cgcgggcaca gaagcacctt
     4501 actcggagct cttcctcaat cacattgccg ggctgcagcg cttcctgccg gcggccatgc
     4561 cgctcttcgc ccccaacgtg aactcctacc gccggctccg ccgccattcc gacgcaccca
     4621 tcaacgtcca atggggctgg gagaatcgca cggtcggcct gcgcgtgccg gaatcgggcc
     4681 ccaaggcccg ccgggtcgag aaccgggtgg ccggggccga cgccaatccc tatctggcca
     4741 tcgccgcctc gctggcctgc ggctggctcg gcatgagcga ggagctgcag ccgaccgagc
     4801 agatcaaggg cagcgcctac cggctggccc agaccctgcc gcagcaactg ccggacggcc
     4861 tgaagaagct gaacagctcg ccgcaactca aggaagtgct cggcgaggcc ttcgtgcgcg
     4921 tcctgatcgc cgtcaagcag gccgagcacg aggcctacca gagcgtgatc agctcctggg
     4981 aacgtgagca cctgctgctc aacgtctgat tccaactctt tgacattccg ggaatatcga
     5041 caatggatgc gcgcgccgat accgaccgcc tgcgggccct cgaccgcgat caccacctgc
     5101 atcccttcac cgtctttccc gagcttcggg ccaagggcag ccggatcatc acccatgccg
     5161 agggctgcta catcttcgac agcaacggca accggatcct cgacggcatg gccgggctct
     5221 ggtgcgtcaa tctcggctac ggccgcgagc ggctggcgcg ggtcgcctat gagcagatgc
     5281 aggagctgcc gttctacaat gccttcttcc agacggcgac cgtgccctcg atcgagctgg
     5341 cccgggccct ggcggaaatc ctgcccgagg gcttcgagcg cgtcttcttc gcctcctccg
     5401 gctcggaggc gaacgacacg gtgatcaagt ccgtctggta ctactggaac ctgatgggca
     5461 agccggcgaa gaagaccttc atcagccgca cgctcggcta tcatggcgtc gggctcggct
     5521 cggccagcct caccggcatg cgcttcatgc acgacccctt cgacctgccg ctggagcgct
     5581 tccatcatat cggcaatccg tattggttcg cggaaggtgg ggatctcgat cccgaggcgt
     5641 tcggcaaggt ggccgccggc tggctcgagg cgaagatcct cgagatcggc ccggagaacg
     5701 tggcggcctt cgtcgccgag ccgatccagg gtgccggcgg cgtcatcatc ccgccatcga
     5761 cctattgggc cgaggtgcag cgcatctgcc ggcaatacga tgtgctgctg gtcgccgacg
     5821 aggtgatctg cggcttcggg cggaccggca actggtgggg ctcgaccacg ttcgacatcg
     5881 caccggacat catcccgatg gccaaggggc tctcctccgg ctaccagccg atcgccgccg
     5941 tggccttcca cgaccggatc gccgacgtgc tcgccgcctc gggcacggag tacgcgcacg
     6001 gcgtcaccta ccacggccat cccgtggcgg cggcagtggc gctcgagacc atccggatca
     6061 tgcgggagga ggggctcgac acccgggcat ccggcccgat cggcgcctgt ttccgggacc
     6121 ggctgatgac gctggccgac cactcgctgg tcggcgaggt gcgcacctgc ggcctcctcg
     6181 cctgcgtcga gctggtcgag gacaaggcgg cgcgccgtcc ctatccggcg gcccgcaagg
     6241 tcgggctcac ctgccgcgag cactgcttcg acaacggcct cgtgatgcgt gcgattcgcg
     6301 atggcatggt gctctccccg ccgctgatcg tcagcgaaag ggagatcgac gagatggtga
     6361 ccaaggcggc cgatgccttc gacctgacgc tccgctcgct cgccggctga ccgctacagc
     6421 agccagaaga gcaggaacgc gccgagcacc agccggtaga tcacgaaggg cgtgaagctc
     6481 gaggtctgga gccacctcat caggccccag atggcgcaga gggcggctac gaaggcgagg
     6541 agcgctgccc agagcgcgtc cgcctgcagc gtcgcgtcgc cgctctgcag caggtcgagc
     6601 gtggcgagca ggccggcggc ggcgatcgtc gggatcgcca gcagcatcga gaagcgggca
     6661 ctctcggtcc ggctgaagcg caggaagagc cccgccgtca tggtgatgcc ggagcggctg
     6721 gtgccgggca ggatcgccag cacctgggca aggccgatga cgatggcatc gcgcagcgtc
     6781 atctgggcga ggctgcggtc atgatgctcg gcgccgcgat cggcgagcca gaggacgata
     6841 ccgaagacga tcgtcgtcac cgcgatgagc aggccgctcc ggaaggtcgt cccgacgaga
     6901 tccttggcca gcagccccgc gacgacgatc ggcagcgtgg cgacggccag atgcaggacg
     6961 agccgcgagc ggtggtcggg ccgccgccgg atcgccagcc agagcccggc caccagatcc
     7021 agcacgtcgc gatggaaata gaccagcacg gccgccagcg tgccgacatg cacggcgatg
     7081 tcgatcgcca gcccctggtc cggccagccg aagagcacgg gcaccaggat gaggtgcgcc
     7141 gatgagctga tcggcaggaa ctcggtgatg ccctggacga ccgccagcac gagcaggttg
     7201 agcagatcca tgcttttatc cgggccccgg tgacctggcc cgccgcggcg gggcgcggct
     7261 tgatcgccac tgcgggattt gccatacccg cagaggtggc cacgccgcaa ctcgcctgcc
     7321 tgccacccgg aacaccagat cttcgaacag cattgtgccg ccggacgctc tttctggcag
     7381 cagccggcgg tgtggctgcg ttgccgggag atgagggacc atgcacgacg atccgatcgg
     7441 cggcgagcac cttgcgccgg tgtccttctc ggccgagaat catccggccg atctcgccga
     7501 gctgctcgag gaaatggcgc ccgaggcggc gcgggtggcg ctgctcgcct tgccgctcgg
     7561 ccatggtgcg gagaccttcg gctatctccc gcccgagatc caggtagcgc tcgcccgggt
     7621 gttgagtcgc ggcgagctcg ccgcgatcgt caccgaaatg aacggcgacg atcgtgccga
     7681 cctctacaac cgcctgaccc ccgagcagca ggaggcgctg ctgccgggcc tcgcccaggc
     7741 cgagcgcgag gacatccgcc gcctcgccgc acatcaggag ggcacggccg gggccatcat
     7801 gacctcggac tatgccacgc tggcgcccgg gctgaccgtg cttcaggcca tcggcaagct
     7861 gcgcctcgag gcaccggaca aggagaccat ctaccgcacc tatgtggtcg atgccgagcg
     7921 ccggttgatc ggctcggtgc ggctgcagac cctgatcctc gcccagccgc agcgtaccgt
     7981 cgaggaggtg atggagcgca ccactcgtgc cgtcggggtc gaggacgacc aggaggaggt
     8041 ggcccggtcg atcgccaagt acgacatcat cgccctgccg gtggtcgacg gcgagggccg
     8101 gctggtcggc atcgtcaccc acgacgacgc cctcgacgtg ctgcaagcgg aggccaccga
     8161 ggacttccag aaagtcggca gcgtcggctc gatggccgac agcatcgcca gcgccagcat
     8221 cgccctgctc taccggagcc ggatctactg gctggtgctg ctggtcttcg gcaacctcct
     8281 ctcgggggcg gggctcgctt tcttcgagga caccatcgcg gcctatatcg tgctcgtttt
     8341 cttcctgccg gtgctcatcg gcagcggcgg caatgctggc tcgcagagct cgacgctgat
     8401 ggtccgcgcc ctggcgactg gcgatgtcga gatgaaggac tggggccgga tgttctttcg
     8461 cgagctcggc gtggcgatcc tgctcggcat gaccatggcg gttgcgatct ccaccatcgc
     8521 cgggtttcgg ggtggtcacc aggtggcgct ggtggtggcg gtctcgatgc tgctgatcgt
     8581 cacggcgggc agcctgatcg gcatgtcgat gccgttcatc ctgagccgct tccggctcga
     8641 tccggccact gccagcgccc ccctgatcgc gtcgatcgcc gacatcatgg gcgtcgtgat
     8701 ctatttctcg atcgccagag cgatcctgct ccattgagaa gcggacggtc ggccgcgagc
     8761 cgtcgcccga acccggatct cgagcaacag ctcgagcccg ccgtcgagca cgacccggcg
     8821 ctcctcgcaa acgtggctga cgaccggatc ctgcgccccg gcgctgcgcg cggcggcgat
     8881 ggccgcttcc tcggcatggc tgatcaggag cgtgcgcgcc gacgccgggt cggtgacgag
     8941 ggttcccgcc tcgccaagat gcagggcgag ctggtcgtcg ccggtgcgcg tggcgaccag
     9001 ctcgacccgg cgcgccacct cgccgaccac ggcgccgatg gcgttgcaga ccgcgtgacc
     9061 ggcgggcagc acgagacggc tgccgagccg ctcggcgacc ccctcgtaga agaccgcagc
     9121 cggcccacct actgccacga gcggtcggtc gatggtgaag cggacggcga gcggtgccct
     9181 gggctcgtcg ccggccaggg ccatggcgac cggtggttcg tccaggaacc ccgcgagacg
     9241 cccgacagtg ccgcctgtcg cttcccagac ggcgccgacc acggcacggg cactggcggt
     9301 gcgtgccgcc tccagcacgg ccgccgcgaa cctctcgacc gtgtcgcctc cgcctcgctt
     9361 gcgtgcctcg agctgggcgc cgagcctcgc cgcctcgatc gaccacccgg actgccggcc
     9421 gagcacatgg cccgcatcgc tcggcgtgaa gccggcgagc ctgacgatgc cctgcaaggc
     9481 gagcgacttc agcggcacgg ccagatgctc gcgatcgatc accagctcga gcgggcaggg
     9541 gccgccggcg agcaggtcca acaggcggtg ctggctccgc gagaggccgg aaagcgcgac
     9601 gtccgcgtcc tggcgaaggg cgaagcgcgc atccgtggcc cgagtggcgg cccgtgccgc
     9661 ctgggcatgg agcgcctcga gcacctgcgg gtgctggtcg gcgagcagcg ccagcggcac
     9721 cagccggcgc ggcccgagcc cgaggcgacc cgcctcgtcg acacgggtct cgctgtcgcc
     9781 gccgagcccg ctcgtctgca gctccaccgc ctcgaccatc gttctgaagc caccgaccag
     9841 ggcaccttcc ggggcgagac gcggctggcc gtcatcgagc agcgccacat cagtggtcgt
     9901 gccgcccaca tcgctgatca cggcatgacg aaggccggag agatgggcgg cgcccaccac
     9961 gctggcggcg gggccggaga ggagggtctc gaccggccgg cggcgggcca cttctgccgc
    10021 catcaaggca ccgtcgccgc gcaccaccat gagcggagcc gcgatacccg tctcggccaa
    10081 cagccgctcg acggccgcga tgagccggcc gatgcgtggc acaagccggg cattgaggaa
    10141 ggtggtgagc gcccggcgcg gcgcgtcgag ccgggcggtg agctcgctcg agacggtgac
    10201 cggccggccg gtcagggcca tgatccgctc ggcggccacc tgctcatgcg ccgggttgcg
    10261 caccgcaaag gtggcggtga ccgcgaaggc atcgacggtc ggcgccatct tgccgatggc
    10321 cgcatcgagg gcggcgaggt cgagcggctg ccgcggctgg gcgctcgcat cgtggccgcc
    10381 gccgacaaag gcgacaggcg cccggccgag tgcctggccg agccccccac gcgccaggct
    10441 ctccgggccg aagcccacca tgatcagccc ggcccgcccg ccctggccct cgaccagcgc
    10501 attggtggcg agcgtggtcg agaggccgac cagaccgatc cgctcgcgag cctcgtgggg
    10561 tgccgcggcc aggacggcgc gcacggccgc ggcgatgccc tggtagaggt cgtggtggct
    10621 ggtcagcgcc ttggcgctgg cgaccacgcc ttccgcctcg tcgagcagca cggcgtcggt
    10681 gcaggtgccg ccggtatcta tcccgagacg catcagccgc catccgcccc cgggcaggtt
    10741 cctcgtcgct ccgatcgcgc cggcctcgcc aagcgatgca aaccttccct tggtcgggca
    10801 aggctgctag ggccggccgg cggaacgcgg atcggcgtag cagagcatgg cgggatcaca
    10861 agggcgaaac gcgatgcttt tcacgatcag aagcaactgg gcgctgctgc tcggcatcgg
    10921 gctcctgatg ctcggccacg gcctgcagaa cacgctgctc ggcgtgcggg ccagcatcga
    10981 aggcttcccc acctcggtta ccggcattgt catgtcgggc tattcgatcg gctttctcgt
    11041 ctccgccatc gtcaccccgc ggctggttgc ccatgtcggc catgtccggg tctttgccgc
    11101 gttcaccgca cttgcctcct cggcgatcct ggcgcacgcc ctcctgctcg agccgatctc
    11161 gtggttcgtg ctgcgcttcc tcacgggtgt gtgcatgagc ggcgcctatg tcgtcgccga
    11221 aagctggctc aaccgggcgg ccaccaacga ggaccgcggc tccctgctct cggtctacat
    11281 gctggtccag ctcaccgcct gggccggcgg ccagctcctg ctcaatgccg ccgaccccgg
    11341 cgggtttcgc ctgttcatcg gggtctccgt cctgctctcc atcgccgtcg tgcccatgct
    11401 gctgacggcg agccccgcac cggcgacggc cgcggcacgc agcttcggcc tgatcaggct
    11461 ctaccgcagc tcgccgctcg gcttcgtcgg catgttcggc gtcggcctca gccagggcgc
    11521 cttcttcgcc atgggtgccg tcttcgccgg caagatcggc ctcgacgtcg ccggcatctc
    11581 gctcttcatg gcgctcgcca tcgtcggcgg cgtgctgctg cagtggccgg tcggccggct
    11641 ctcggaccgg ctcgaccggc gccgggtggt gaccgggacg accatcctcg ccgggctcgc
    11701 caccggcatc gtcgtcattg ccggcacggg cacgttcccg gtcgtcgctg ccgcgttctt
    11761 cctctacggc ggcatgtgcc tgccgatgta tgcgctctgc atcgcccaca ccaacgacaa
    11821 tctcgacgcc gaggacatgg tgggcgccag cggcacgctc gtcctcgtcg tcggcatcgg
    11881 catggtgctg ggcccgccct tggcggcggc ggccatggac ctgcagggcc cggtcggctt
    11941 cgcgtccttc ttcaccatcg tccatctcgg catcggcgcc ttcgcgctct accggatgac
    12001 ccggcgcgca tcggtgccca tggcggacca gggacagcac atctatgcgc cgcagccgag
    12061 cccggtagcc gcggccctcg tccaggaaac ggcgatcggc cagcaggaag cgaaggaaca
    12121 gaccgagatc gtgcaatctc cgctcgcttc gcccgccggt caggggtata attagctggt
    12181 ctgtcagtgc tgaaggtcgc tttcgaaact tgtccgcgtc cagtattcgc agcgtttatt
    12241 ccgagcgcgc tcgaacgaaa tcccgccgtc gcgaaccctt ctcgtcgcca tttcgtggcg
    12301 acggcgttcg gtcgtgcgtt gtggatcagg tggcggtcag ggcttcgtgc tttacctcag
    12361 ggcttggcct ggactccttg tatctccatt caggagcggc tcgatgagca gcaagatctt
    12421 tgtcgctaac ttccccttca gcacgacgtc cgaggacctc caggacctct tcggacagca
    12481 cggcgaagtg gtgtccgcca aggtcgcgac cgaccgggaa accggccgga gtcgtggctt
    12541 cggcttcgtc gagatggcga cgccggcggc cgccggggcg gccatccagc agctcaacgg
    12601 ctacgacatg ggcggccggc ccctggccgt gcgtgtcgcc gaggaccgcg gcggtgccgg
    12661 cggcggcggg ttcggtggcc gaggcggccc gggtgggggg cgcggtcccg gcggacccgg
    12721 cggcggaccg cgccgcgaca atctcggacg gccgccgcgc cgcccgtact gagcctgctt
    12781 ctggtccggg gcgaccgggc ctcctgcggg cgacccggcg cctgctgccc gttgcagcga
    12841 ccgcctcgcc cgcctacatg acggcggggc ggctggccgc ctctgcggcc actggggccg
    12901 aacccggaca tcattgatga tcgtcgagct caacgcacgt tcgcgggaag tgttccgcca
    12961 gatcgtcgac gcctacatgg cgaccggcga gccggtcggc tcgcgcacgg tctcgcgccg
    13021 gctcgacgac cagctctcgc cggccaccgt ccgcaatgtc atggccgatc tcgaggaggc
    13081 cggcctgctc tgcgcgccgc acacctcggc cggtcgcctg ccgaccgatg cgggtctcag
    13141 gctctatgtc gatgggctgc tcgagctcgg tggcctctcc aaggacgacc ggcagcgcat
    13201 cgagacgcaa tgcgccgtgg ccgggcggag catcgaggcc gtgctcgagg acgcgaccga
    13261 gacgctctcc ggcctctcgc gctatgccgg cctcgtcatc gcccccacga ccaggcgacc
    13321 ctacaagcac atcgagttcg tgcccttgag ccccggccgg gcgctggtgg tcaccgtcag
    13381 cgatggcggc atggtcgaga accgggtca
//
LOCUS       DAOTFI010000135        13403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5519_length_13403_cov_7.111852, whole genome shotgun sequence.
ACCESSION   DAOTFI010000135 DAOTFI010000000
VERSION     DAOTFI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5519_length_13403_cov_7.111852"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="P5558_14300"
     CDS             complement(<1..443)
                     /locus_tag="P5558_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25533.1"
                     /translation="MPAWHVLDLLTLAGSGRISAVRLDSLETPGRERSGAVLWARPAR
                     SSVLATLQFALLCAALIWLTVRGAANMGYNWQWYRVPDYFARVVDERLVWGPLAFGFV
                     ETIRLSAIAFVLTVLLGLVVAVLRLSRSPVGRAVAIGYTELIRNTP"
     gene            575..1210
                     /locus_tag="P5558_14305"
     CDS             575..1210
                     /locus_tag="P5558_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRY25534.1"
                     /translation="MLLLPFWLDAAGVFVFAVSGALTASRKELDILGFVFIGSVTGIG
                     GGTVRDLLLGRIPVFWVGQPDYLLVTVAASVLVYFTAHLVERRYKLLLWADALGLALF
                     SVLGARIALLAGTGPLVAIVMGVMTAAMGGLIRDVVCGERPLILAREIYVTAAVAGAA
                     VCVLLLARGSPVLLAEALGVLAAFAIRGAAIRWSLELPTYRSRPGRDYTQR"
     gene            complement(1223..2164)
                     /locus_tag="P5558_14310"
     CDS             complement(1223..2164)
                     /locus_tag="P5558_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25535.1"
                     /translation="MPGFRLASFATERLGARGGPLVAERRVLQALALRAADADLVALQ
                     GVTSAAALERFRQGELARAGGLDYRQALLIEGNDPRAGHPALLGRLPIVHARSHAGIG
                     FAEIGRAPPIGTPPEAPVFSRDCLEVETEVQGRRLALFVCHFNDPAPSPDLAGAGSFD
                     AGEGPRERRLAEAAAVRRIIERRWADPARAEWVVLGSLGDEALDDGGRPLPNSGLAPL
                     VADGFAVDLLAQSIAVPADRWTWHRAATGTYRQHDHLLLSPALARRNRQAQPWLVRGG
                     LAWCAERHGGPRFPRVGWLEPAASSHCPVVVDLAFDG"
     gene            complement(2300..3190)
                     /locus_tag="P5558_14315"
     CDS             complement(2300..3190)
                     /locus_tag="P5558_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25536.1"
                     /translation="MRPTVYIHTNHKQHIGALVSAHSMRRNSRHADKFDVELIELKDY
                     EDFFKPYEGRDYLRDGVSRTWLYNDLQSFTPLRFTPPERMGYAGRAVVVDPDVFAVQD
                     VWELLDRDMAGKAIWCCNRSGHPASSVMLLDCAKLPHWKLADNFEALFRREREYKKWM
                     NLGYEDPATIGTLEPVWNDYDRLAPDTRMIHNTRRRTQPWKSGLPVDFVPAEGNPYSP
                     VAWAMYLRRRLFGTYAFLGNYKPHPDKAQENFFFGLLKECVENGTISEALIKEHMKEN
                     HVRHDAFEVMEKVPDLPKAA"
     gene            complement(3335..4438)
                     /locus_tag="P5558_14320"
     CDS             complement(3335..4438)
                     /locus_tag="P5558_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25537.1"
                     /translation="MDLTAKGLVSDIVIDGAAERLEVASLTHAFGATTVVDHVSFVVP
                     AGEVHCLMGPSGCGKTTTLRLIAGLETVQAGTIRLGGAVLADATASLPPERRQIGLMF
                     QDLALFPHLTVAENVAFGIRAMPRGARGDRVKQLLDMVGMTRHAAKHSYQLSGGEQQR
                     IALARALAPRPRLMLLDEAFSALDASLRAEIRAEALQILKEAGVPTLLVTHDPEEAIV
                     AGDRVHVMQAGRLIQSGPADSLYKHPVNEFVANFFGPILRFATKVKGGLAWTPLGMIA
                     APQHADGAPVSVVFRAEALQLMPDYAGKGHHAGQVVGCRRMGPACDLSLRLETGSTIK
                     VRKPAETAHRIGMRMGLEIDQRYTFVFPQADAA"
     gene            4682..6358
                     /locus_tag="P5558_14325"
     CDS             4682..6358
                     /locus_tag="P5558_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY25538.1"
                     /translation="MPSAQQPATAAPMIAGRPTGAAWLVGVAMLAALLVILPLLAVLA
                     NLLAPTTDAWTHVAGRLVPRYLLNTVWLALGVGALTTAVGVGAAWLVVFYRFPGARLV
                     EWALILPLAIPAYVLTYAYYDLLGFSGPVQSAIRAATGLGPRAYWFPNLATVGGAVFI
                     LALAFYPYVYLAARAAFLLQSGSLLAAGRMLGCSQAGAFFRVALPLARPAIAVGVVLV
                     LMETLADYGAVSLLGVQTFTTGIYRAWFSLGDRVAAAQLGAILLGFVLLLVLLERLFR
                     RGRAFNPTSEHRFDDRHPLHGWQAAGALGLCLVPLGLGFALPVGRLVWMTIDTKSGIG
                     LARFVELTSNTVSVAAVAAAIAVAVGLVIAYATRLARSPLVPLAAAVAAMGYALPGPI
                     IAIGTLVPLAAFDNALDGWLRASFGVSTGLLLTGSIAALVFAYLVRFLAVSIGHIEAG
                     FARIRPSLDDAASVLGTSVWTRLWRIHMPILWPALGTAALITFVDVMKELPATLVLRP
                     FDFDTLAVRVYNLARDERIAEAALPALVLVLVGLIPVYLLSRGIAKSRRD"
     gene            complement(6433..7461)
                     /locus_tag="P5558_14330"
     CDS             complement(6433..7461)
                     /locus_tag="P5558_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY25539.1"
                     /translation="MSTRRFMLGAASCLGLLAGAASAAEVNVYSARHYDTDAQLYSDF
                     TKETGIEINLIEGNAEELVERIKLEGANSPADVLITVDAGRLWRADQEGVFQPLRSAV
                     LEERIPAEFRHPDGDWFGYTTRARLIFYNKETADPALAQTYEDLARPELEGELCIRSS
                     SNIYNLSLMGSLIEHLGEDAAAEWAKGVAGNLARPPEGGDTDQIVAVANGLCDFAVAN
                     HYYYVRLMTSDDPADREVAAKVGVIFPNQADRGTHVNISGAGMVATSPNPENARAFLE
                     YLASDSAQTYFIAGNSEFPTVPGVAFENPALEEIGAMDFKRDTVPVAVFGENQPLAQV
                     LMDEAGWK"
     gene            complement(7572..9848)
                     /locus_tag="P5558_14335"
     CDS             complement(7572..9848)
                     /locus_tag="P5558_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRY25540.1"
                     /translation="MLDAANLEAVVPPDHPLRGIARILDEVLDGEAAATKVVAAAVAP
                     DLAARPVVALRLLSLLYGLRDIALVVPMLRYDLAMRWFADPAASLDLKTSGSLHRLAE
                     QAIRLPAMRHLVGTILANPHVAELARDVRLRPNHTAIAAAVREGRRAARRRRGRLWIQ
                     VAAALLILLVVAPVVYAGWALPTADELRALLGRPSLVVDIGADHPDRGRAIVAVPVDR
                     AALPQAFVDVLLTAEDRRFYWHPGFDPIGMARAMLAAIQGNLGGEGGAVQGGSTLTQQ
                     LAKNAFLNPARTLRRKLDEMLLAIRLELMFSKDELLELYLANAYFGDGVNGIELAARD
                     YFGKRAAELDLAEAALLAQLLPAPERRRPDLHWPMARERALALLGMVQSEHPEHATAI
                     ALARQRLTAASLAALGRGRRDFRRVELRWFRDWVLGELPALFPDAAGEHRLVLTLEPL
                     AQIYAQIAIERLVQQGRNLGYAEAAVVVMRPNGRVVALVGGRSYAASQFSRATAARRQ
                     PGSAFKPVVYLAALEAGWTLGRRVSDRPYGADGWPRNYRGGHQGEVSMLQAFRQSLNG
                     AAVQLAEAVGRPQIVATARRLGLEGPFPDKPSLALGVAEVTLLELTAAYAVFANGGVL
                     ARPTGVVALRDDTGRPVAWPVERASPIVIQPEPARAMNQLLRSAVEPGGSGSLANIDR
                     HVAGKTGTTQANRDAWFIGFTGDFVVGIWVGNDDGRPMKPEVTGGNVPARAFANLMAN
                     LREDLLGGRTTPPFGLGN"
     gene            10013..10897
                     /locus_tag="P5558_14340"
     CDS             10013..10897
                     /locus_tag="P5558_14340"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HRY25541.1"
                     /translation="MSFQNIVAQLLQQGMGGQTQQRLQHTASQSGGGLDQMLGALLGG
                     NSGGGQAAPADRASGAAAAAGGTGGGVGGLAGGLGGLGGLGKLAQDFLGSQQAGGMSG
                     AQVGGLGALVGAVLGGGGGALKGAAGGGAMAILGSLALTALKNYQANAAASTEAAPAQ
                     RMAPPPVTREEVADVTDPRTERLVLRAMIDAAKADGKVDQDEMQRIIGQIEPGSVTPE
                     ERQYVIDEMSRPADPGGLAREVRSPAVAAEVYAASILAIHIDTEAERAYLRNLATALR
                     LDAGTVQRLHQMTGAPAV"
     gene            10944..12734
                     /locus_tag="P5558_14345"
     CDS             10944..12734
                     /locus_tag="P5558_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRY25542.1"
                     /translation="MIPAELQPWFVLALVVLVFLGFVRERLPAEIIAGLAVLALLATG
                     ILATDELLGVFGNAAPVTVAAMFVLSAALERTGFIDRMGRLVRSAARLSPGMAIALVM
                     TATMLLSAFINNTPVVVILIPIVVSLARTMGISPAKLLMPVSYAAIFGGTTTLIGTST
                     NILVDGVAQARGLAPFGMFEITLAGLALGVVGIGYMVTIGRHLLPDRDSLAALLPDGS
                     QRRFLVDALIPQDSPHVGKSLADAGFQRERGYRVIDVIRGDQSLRRDLDAVLLAGGDR
                     LVLRSRIADVLALKETGHVAFGGEGENAVEPIGSRETVLVEGIVGPGSGFTGRRIADL
                     MLRRRYGVYPLAMHRQGENVPGRFDEVKIRLGDTILLEGAAQGIKMLVDEGQIINLIQ
                     TDARPARRDRAWIAIGAIVLVMLLAAFEVMPIAGLALIAAIAVVATGCVDADEAYGAI
                     RWNILMLIFAMLALGIAMDKTGSVALVVDNLVLLLGGLGPWAMLAALYFMTSAITEIV
                     SNNATAILMAPIAIGLADTLGVDARPFVVAVMLAASASFATPIGYQTNLLVYNAGGYR
                     FGDFLKVGLPLNLLMWLTACLVIPLLWPLS"
     gene            complement(12739..13104)
                     /locus_tag="P5558_14350"
     CDS             complement(12739..13104)
                     /locus_tag="P5558_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25543.1"
                     /translation="MVDLDLLEARLLRAALAGRAETYGSLLAFFERRVTPVMVAALCK
                     DLGRVCDRIEASGGPDLACLVVRASDGLPGAGYFNSLRQAGLYDGEPTGPAAERFVAR
                     AQRRATVYARRRMQDGGRS"
     gene            13199..>13403
                     /locus_tag="P5558_14355"
     CDS             13199..>13403
                     /locus_tag="P5558_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease ydgD precursor"
                     /protein_id="HRY25544.1"
                     /translation="MRRLLVLVSLLVAVSATAGPLAATTPLGIFGDDDQHIIDAADRR
                     WNAVGRLNRESGGFCTAVLIGPKE"
BASE COUNT         1902 a         4929 c         4498 g         2074 t
ORIGIN      
        1 ggcgtgttgc ggatcagctc ggtgtagccg attgccacgg cgcggccgac cggcgatcgg
       61 gaaagccgca gcaccgcgac gacgagcccg agcagcacgg tcagcacgaa ggcgatcgcc
      121 gagagccgga tggtctcgac gaagccgaag gcgagcggcc cccagaccag ccgctcgtcg
      181 acgacgcgcg cgaaatagtc cggcacacgg taccactgcc aattgtagcc catattggcc
      241 gcacccctga cggtcagcca gatcagggca gcgcagagca gggcgaactg cagcgtcgcc
      301 aggaccgacg accgtgccgg ccgggcccaa agcaccgccc cgcttcgctc gcgtccgggc
      361 gtctccaggg aatccagacg tacagcgctg atgcggccac tccccgccag ggtcaggaga
      421 tcaaggacgt gccatgctgg cacccttccc gtgtccggca cgatgctgcg ctgcgccaac
      481 cttgtcaaac gcccggctgg cgcccggccg ccgaccgtgc gattcgctgg cgggggcatt
      541 ccccgacccg tggcatccac cgttggagac gaccatgctg ctgctgccgt tctggctcga
      601 tgccgcgggg gtgttcgtct ttgccgtcag cggtgcgctc accgcctcgc gcaaggagct
      661 ggacatcctg ggattcgtct tcatcggctc cgtcaccggc atcggcggcg gcacggtgcg
      721 cgacctcctg ctcggccgca ttccggtctt ctgggtcggc cagccggact atctactggt
      781 caccgtggcg gcctcggtcc tcgtctactt caccgcccat ctggtcgagc gccgctacaa
      841 gctcttgctc tgggccgacg ctttgggcct cgccctcttc tctgtcctcg gcgcccggat
      901 tgcgctcctc gccggcaccg gccccctggt cgccatcgtc atgggggtga tgaccgccgc
      961 catgggcggg ctgatccgcg acgtggtctg cggcgagcgg cccctgatcc tcgcccggga
     1021 gatctacgtc accgccgccg tcgccggggc cgcggtatgc gtcctgctcc tcgcccgggg
     1081 ctcgccggtc cttctcgccg aggcgctcgg cgtcctcgcc gccttcgcca ttcgcggcgc
     1141 cgcgatccgc tggagcctgg agctgccgac ctatcgctcg cggcccggac gcgactacac
     1201 gcagcggtag cggcgctgcc ggtcagccgt cgaaggcgag atccacgacg accgggcagt
     1261 ggctgctggc ggccggctcg agccagccga cccgcgggaa acgcgggcca ccgtggcgct
     1321 cggcgcacca ggccagaccg ccgcgcacca gccagggctg ggcctgtcga ttgcgccgtg
     1381 ccagggccgg cgagagcagc aggtggtcgt gctggcggta ggtgccggtg gcggcgcggt
     1441 gccaggtcca gcggtccgcc ggcacggcga tgctctgggc caggagatcg acggcgaagc
     1501 catcggcgac gagaggtgcg aggccgctgt tcggcagagg gcgcccgcca tcgtccagcg
     1561 cctcgtcgcc gaggctgccg agcaccaccc attccgccct ggccgggtcg gcccagcgcc
     1621 gctcgatgat ccggcgcacg gccgccgcct cggcgagccg ccgctcgcgg ggaccctcgc
     1681 cggcgtcgaa cgaacccgcg ccagccagat cgggtgaggg ggcaggatcg ttgaaatggc
     1741 agacgaagag ggccaggcgc cgcccctgca cctcggtctc gacctcgagg cagtcgcggc
     1801 tgaagacggg cgcttcgggc ggcgtgccga tcggcggcgc gcggccgatc tcggcgaagc
     1861 cgatgccggc atggctgcgt gcatgaacga tcggcaggcg gccgagcagg gccggatgcc
     1921 cggcacgggg atcgttgccc tcgatcagca gcgcctggcg atagtcgaga ccgccggctc
     1981 gggcgagctc accctggcga aaacgctcga gcgccgcagc gctcgtcacg ccctgcaggg
     2041 cgacgagatc cgcatccgcg gcgcggagcg ccagcgcctg cagcacccgc cgctcggcga
     2101 ccagcgggcc gccgcgcgcg ccgagccgtt ccgtcgcgaa gctcgcgagc cgaaagccgg
     2161 gcatcgcgcc tctccccatg cagtgcctgc cgtgcatgga aacgaggcta gcggggcttg
     2221 gttgccagcg gcttagcgtc ggcggaaatc gttcagggcg cgtgaagaaa gggtcgcccg
     2281 cgcctagcgg gctacgcctt caggcggctt tcggcaggtc cggcaccttc tccatcacct
     2341 cgaaggcgtc gtggcgcaca tggttctcct tcatgtgctc cttgatcagc gcctcgctga
     2401 tggtgccgtt ctcgacgcac tccttcagca ggccgaagaa gaagttctcc tgcgccttgt
     2461 cggggtgcgg cttgtagttg ccgaggaagg cgtaggtgcc gaagagccgc cggcgcagat
     2521 acatggccca ggcgaccggc gagtaggggt tgccctcggc cgggacgaaa tcgaccggca
     2581 ggcccgactt ccagggctgg gtgcgccgcc gtgtgttgtg gatcatcctc gtgtcggggg
     2641 cgagccggtc gtagtcgttc cagacgggct cgagcgtgcc gatcgtggcc gggtcctcgt
     2701 agcccagatt catccacttc ttgtattcgc gctcgcggcg gaacagggcc tcgaaattgt
     2761 cggcgagctt ccagtgcggc agcttcgcgc aatcgagcag catcaccgag gaggccgggt
     2821 ggcccgaccg gttgcagcac cagatcgcct tgcctgccat gtcgcggtcg agcagctccc
     2881 agacgtcctg cacggcgaac acgtccgggt cgacgaccac ggcgcggccg gcatagccca
     2941 tccgctcggg cggggtgaag cggagcggcg tgaacgactg caggtcgttg tagagccagg
     3001 tccggctgac cccgtcgcgc agatagtcgc ggccctcgta gggcttgaag aaatcctcgt
     3061 agtccttgag ctcgatcagc tcgacgtcga acttgtcggc atgcctggaa ttgcggcgca
     3121 tcgaatgggc cgagaccagc gcgccgatat gctgcttgtg gttggtgtgg atgtagacgg
     3181 tggggcgcat cgccgatact ccaggattcg tcgcgtcgaa gggggttgac aggcgatcaa
     3241 ttaatactgt ggccgggcgc caccaagtga cggttgcgcg aatgcgaacg gttccagggg
     3301 cgacgtgacg tcgctgccgc agccggcgga gccgtcaggc ggcgtcagcc tgcggaaaca
     3361 cgaaggtgta gcgctggtcg atctcgagcc ccatccgcat gccgatgcgg tgcgccgtct
     3421 cggccggctt cctgaccttg atggtgctgc cggtctcgag ccgcaggctg aggtcgcagg
     3481 ccggacccat gcgccggcag ccgaccacct ggccggcatg atgccccttg cccgcgtagt
     3541 ccggcatgag ctgcagggcc tcggcgcgga agacgaccga gaccggcgcc ccgtcggcgt
     3601 gctgcggggc ggcgatcatg ccgagcggcg tccaggcgag cccgcccttc accttcgtgg
     3661 cgaagcgcag gatcgggccg aagaaattgg cgacgaactc gttgaccgga tgcttgtaga
     3721 ggctgtccgc cgggccgctc tggatcagcc gcccggcctg catcacgtgc acccggtcgc
     3781 cggcgacgat ggcctcctcc gggtcgtgcg tgaccagcag ggtcggcacc cccgcctcct
     3841 tcaggatctg cagggcctcg gcgcggatct cggcgcgcag gctggcatcc agcgccgaga
     3901 acgcctcgtc cagcagcatc agccgcggcc gcggcgccag cgcccgggcc agggcgatgc
     3961 gctgctgctc gccgccggag agctggtagg aatgcttcgc cgcatggcgc gtcatgccga
     4021 ccatgtcgag cagctgcttc acccggtcac cgcgggcacc cctcggcatc gcgcggatgc
     4081 cgaaggcgac gttctcggcg accgtcagat gcgggaagag ggcgaggtcc tggaacatca
     4141 gcccgatctg ccgccgctcc ggcggcagcg aggccgtggc gtcggcgagc accgcaccgc
     4201 cgagccggat cgtccctgcc tgcaccgtct cgagcccggc gatcagcctg agcgtcgtgg
     4261 tcttgccgca gccggacggg cccatcaggc aatgcacctc gccggcgggc accacgaagc
     4321 tcacatggtc gacgacggtg gtggcaccaa acgcatgcgt cagcgacgcg acctcgaggc
     4381 gctccgcagc accatcgatc acgatgtcgc tcaccagacc cttggccgtc agatccatcc
     4441 accgaaccga aatgagacat ccccggctat ggtgcgggca acagcgatca acgcagcgcc
     4501 cgcggccacg ccacccaccc ttctaccgct tcgccccgca gctcgatagg gcgccgtgcc
     4561 acaccgggcg ttagaaatgt tcaaaaacac ccgccgaata ccgcatcccg gccgctcagc
     4621 cgctatctgt tgcgccaccg acccgaccgt cgccgccgaa catgaacgtc cggatgtccc
     4681 gttgccgagc gcccagcagc ccgccactgc cgcccccatg atcgccggcc gcccgaccgg
     4741 tgctgcctgg ctggtcggcg tcgccatgct ggcggcgctc ctcgtcatcc tgccgctgct
     4801 ggcggtgctg gccaacctcc tggcaccgac caccgacgcc tggacgcacg tcgccggccg
     4861 gctggtgccg cgctacctcc tgaacacggt ctggctggcg ctcggcgtcg gcgcgctgac
     4921 caccgccgtc ggcgtcgggg ccgcctggct cgtcgtcttc taccgcttcc ccggcgcccg
     4981 gctggtcgaa tgggcgctga tcctgccgct cgccatcccg gcctacgtgc tgacctacgc
     5041 ctattacgac ctcttgggct tttccgggcc ggtccagtcg gcgatccgcg ccgccaccgg
     5101 cctcggcccc cgcgcgtact ggttcccgaa cctcgccacg gtcggcggtg ccgtcttcat
     5161 cctggccctc gccttctacc cctacgtcta tctcgccgcc cgggccgcct tcctcctgca
     5221 gagcggcagc ctgctcgcgg ccggccgcat gctcggctgc agccaggccg gggcgttctt
     5281 ccgggtcgcc ctgccgctcg cccggccggc gatcgccgtc ggcgtggtgc tggtgctgat
     5341 ggagaccctc gccgactacg gcgcggtgtc gctgctcggc gtgcagacct tcaccacggg
     5401 catctaccgc gcctggttct cgctgggcga ccgggtggcc gcggcccagc tcggcgccat
     5461 cctgctcggc ttcgtcctgc tgctggtgct gctcgagcgg ctgttccggc gcggccgcgc
     5521 cttcaacccg acctcggagc accgcttcga cgaccgccac ccgctccacg gctggcaggc
     5581 cgcaggcgcg ctcgggctct gcctcgtgcc gctcgggctc ggcttcgccc tgccggtcgg
     5641 ccggctcgtc tggatgacca tcgacacgaa gagcggcatc ggtctcgccc gcttcgtcga
     5701 gctcacctcg aacaccgtga gcgtggcggc ggtggcggcg gccatcgccg tggccgtggg
     5761 cctcgtcatc gcctacgcca cccgcctcgc ccgctcgccg ctcgtgccgc tcgccgcggc
     5821 ggtcgccgcc atgggctacg cgctgccggg cccgatcatc gccatcggca ccctcgtccc
     5881 gctcgccgcc ttcgacaacg ccctcgacgg ctggctgcgc gccagcttcg gcgtctccac
     5941 cgggctcctg ctcaccggca gcatcgccgc cctcgtcttc gcctatctcg tccgcttcct
     6001 cgcggtctcc atcggccata tcgaggccgg cttcgcccgc atccggccga gcctggacga
     6061 cgccgcctcg gtgctcggca cctcggtctg gacgcggctc tggcggatcc acatgccgat
     6121 cctctggccg gcgctcggca cggcggcgct gatcaccttc gtcgacgtca tgaaggagct
     6181 gccggcgacc ctggtgctca ggccgttcga cttcgatacg ctggccgtcc gcgtctacaa
     6241 cctggctcgc gacgagcgca tcgccgaggc ggccctgccg gccctggtgc tcgtgctggt
     6301 cggcctgatc ccggtctacc tgctctcccg cggcatcgcg aagagccgcc gcgactgatc
     6361 atcgccacgc cgaaacgcga gacgccccgg cgagccgggg cgtccacgcg aggtccgatc
     6421 gtcaggccgg tgctacttcc agccggcctc gtccatcaac acctgagcca gcggctggtt
     6481 ctcgccgaaa accgccacgg gcacggtgtc gcgcttgaag tccatggcac cgatctcctc
     6541 gagcgccgga ttctcgaacg ccacgcccgg caccgtcggg aactcgctgt tgccggcgat
     6601 gaagtaggtc tgcgccgaat cgctcgccag atactcgagg aacgcccggg cgttctcggg
     6661 attgggcgag gtcgccacca tgccggcacc cgagatgttc acgtgcgtgc cgcggtccgc
     6721 ctggttcggg aagatcacgc cgaccttcgc cgccacttcc cggtcggccg gatcgtccga
     6781 ggtcatcagc cgcacgtagt agtagtggtt ggcgacggcg aagtcgcaga gcccgttggc
     6841 caccgccacg atctggtcgg tgtcgccacc ctccggcggc cgagcgagat tgcccgccac
     6901 gcccttcgcc cactcggccg ccgcgtcctc gcccagatgc tcgatcaggc tgcccatcag
     6961 cgagaggttg tagatgttgc tgctcgagcg gatgcagagc tcgccctcga gctccggccg
     7021 cgccaggtcc tcgtaggtct gggccagcgc cgggtcggcc gtctccttgt tgtagaagat
     7081 cagccgtgcg cgggtcgtgt agccgaacca gtcgccgtcg ggatggcgga actccgccgg
     7141 gatccgctcc tcgagcaccg ccgaccgcag cggctggaag accccctcct ggtccgcccg
     7201 ccagagccgg ccggcatcca ccgtgatcag cacgtccgcc gggctgttgg ccccctcgag
     7261 cttgatccgc tcgaccagct cctcggcatt gccctcgatc aggttgatct cgatgccggt
     7321 ctccttggtg aagtcgctgt agagctgcgc gtcggtatcg tagtgacgcg cgctgtagac
     7381 attgacctcg gcggcgctcg ccgccccggc gagcagcccg agacagctcg cggcgcccag
     7441 catgaaacgt cttgtcgaca cggtgcagaa tcctccggct gcgttgatcg aaccggccgt
     7501 gcgcttcgca cggcggccac ccgcccggca atcgaaagcg ctgcagcctg gatttggtat
     7561 agctctaaat ctcagttccc taggccgaag gggggcgtcg tcctgccacc caacaggtcc
     7621 tcacgcaagt tcgccatgag gttggcgaag gcccgcgccg gcacgttgcc gcccgtcacc
     7681 tcgggtttca tcggccgacc atcgtcattg cccacccaga tgccgaccac gaaatcgccc
     7741 gtgaacccga tgaaccaggc atctcggttg gcttgggtcg tcccggtctt gcccgcgaca
     7801 tggcgatcga tattggcgag gctgccgctg ccgcccggct ctaccgcgct gcgcaatagc
     7861 tggttcatcg cccgtgccgg ttcgggctgg atgacgatcg gcgaggctcg ctcgaccggc
     7921 catgccaccg gccggccggt gtcgtcccgg agcgccacga caccggtcgg ccgagccagg
     7981 acgccaccgt tggcgaacac ggcataggcc gccgtcagct cgagcaaggt tacttcggcc
     8041 accccaaggg cgaggctcgg cttgtcaggg aatggcccct cgagcccgag gcgccgggcg
     8101 gtggcaacga tctgcggccg gccaaccgcc tcggccagct gcacggcggc accgttcagt
     8161 gactggcgaa atgcctgcaa catggagacc tcgccctgat ggccacctcg atagttgcgc
     8221 ggccagccgt cggcgccata ggggcgatcg ctgacccggc gaccgagtgt ccatcctgcc
     8281 tccagcgccg ccaggtaaac caccggcttg aacgcggagc ccggttgccg tcttgccgcc
     8341 gtcgcccgcg agaactggct cgccgcatag ctgcgccccc ccaccagggc gacaacccgg
     8401 ccgttcgggc gcatcaccac caccgccgct tcggcatagc ccagattccg gccctgctgg
     8461 acgagccgct cgatcgcgat ctgcgcgtag atctgggcca gcggctccag ggtcaagacc
     8521 aggcggtgct cgccggccgc atcgggaaac aacgccggca gctcaccgag cacccagtcg
     8581 cggaaccagc gcagctcgac gcggcggaag tcgcgccggc cacggccgag agccgccagc
     8641 gatgccgcgg tcagccgttg ccgggcgagg gcgattgccg ttgcgtgctc gggatgctcg
     8701 gactgcacca tgcccaggag tgccagcgcc cgctcgcgcg ccattggcca gtgcaggtcg
     8761 ggccgccgcc gttccggcgc tggtagcagc tgcgcgagaa gcgccgcctc cgccaggtcc
     8821 agctcggcgg cccgcttgcc gaaatagtcg cgtgcggcca gctctatgcc gttcacgcca
     8881 tcgccgaaat aggcattggc gagatacagc tcgaggagct cgtccttgct gaacatcagc
     8941 tccaaccgga tcgcgaggag catctcgtcg agcttgcgcc gcaaagtgcg ggccgggttg
     9001 aggaaggcat tcttggccag ctgctgcgtc agcgtgctgc ccccctgaac agcgccgccc
     9061 tccccgccga ggttgccctg aatcgcagcc agcattgccc gcgccatgcc gatcgggtcg
     9121 aacccgggat gccagtagaa acgccggtcc tcggctgtca gcagcacgtc gacaaaggct
     9181 tggggcagtg ccgcccggtc gaccggcacg gcaacgatcg ctcgaccgcg atcggggtgg
     9241 tcggcgccaa tgtcgaccac gaggctcggc cgaccgagca gcgcccgcag ctcgtcggcg
     9301 gttggcagcg cccagccggc gtagacgaca ggcgccacca ccaagaggat cagaagcgcg
     9361 gccgccacct ggatccagag acgacctcgg cgccggcgcg cggcccgccg cccctcgcgg
     9421 acagcagcgg cgatcgccgt gtggttagga cgcagccgga catcacgggc aagctcggcg
     9481 acgtgcggat tcgctaggat cgttcccacg aggtggcgca ttgccggcag ccgaatcgcc
     9541 tgctcggcca ggcggtgcag gctgccactg gtcttgaggt ccagcgaggc cgcagggtcg
     9601 gcaaaccaac gcatggcgag gtcgtagcgc agcatcggca cgaccagagc gatgtcgcgc
     9661 aggccataga gcaggctcaa gagacgcaac gccacgaccg gccgcgcagc caggtccggt
     9721 gcaacggctg cagccaccac tttggtggct gcagcctcac cgtccagcac ctcgtccagg
     9781 atccgcgcaa ttcctctcag cggatgatct ggcggcacca ccgcttcgag gttcgccgcg
     9841 tcgagcatga cgtcagcccg ttatcgttcg atgatggttt caccgcccct ctcccatctt
     9901 gacgggtggc gggaccggtg acaatgccgc cggggttgca catcgctctt cgctcgacgc
     9961 gaacccccac ctctcctatt tcagttgagg caggaaaggg agattcggtg ccatgagctt
    10021 ccagaacatc gtcgcgcagt tgctgcagca gggcatgggc ggccagacgc agcagcgtct
    10081 gcagcacacg gccagccagt ccggcggcgg cctcgaccag atgctgggtg ccctgctcgg
    10141 cggcaactcg ggcggcggcc aggcggcgcc ggcggaccgg gcgagcggcg ctgccgccgc
    10201 cgccggcggg acgggtggcg gcgttggcgg gctggcgggc gggctcggcg gccttggcgg
    10261 gctcggcaag ctggcgcagg acttcctcgg cagccagcag gcgggcggca tgtcgggcgc
    10321 gcaggtcggt ggcctcggtg ccctggtcgg cgccgtgctt ggcggtggtg gtggtgccct
    10381 caagggtgcc gccggcggcg gcgccatggc gatcctcggc agcctggccc tgaccgcgct
    10441 caagaactac caggccaatg ccgcggccag caccgaagcg gccccggcac agcggatggc
    10501 gccgccgccg gtgacccggg aggaggtggc cgacgtcacc gatcccagga ccgagcggct
    10561 ggtgctgcgc gccatgatcg atgcggccaa ggccgacggc aaggtggacc aggacgagat
    10621 gcagcgcatc atcggccaga tcgagcccgg ctcggtcacg cccgaggagc ggcagtacgt
    10681 gatcgacgag atgagccggc cggccgatcc cggcggcctc gcccgcgagg tccggagccc
    10741 ggcggtcgcc gccgaggtct acgccgcctc gatcctggcg atccatatcg acaccgaggc
    10801 cgagcgggct tatcttcgca atcttgcgac cgcgcttcgg ctcgatgccg ggacggtcca
    10861 gcgcctgcac cagatgaccg gcgcacccgc ggtctgagcg ccaaccgcgg tgccgtgacg
    10921 aggggtgcca tgacgaggac tggatgatcc cggccgagct gcagccctgg ttcgttctcg
    10981 ccctcgtcgt tctggtcttt ctcggcttcg tccgcgagcg gctgccggcc gagatcatcg
    11041 ccgggctcgc cgtcctggcg ctgctcgcga ccggcatcct cgccaccgac gagctgctcg
    11101 gtgtcttcgg caacgccgca ccggtcacgg tggcggcgat gttcgtgctc tcggcggcgc
    11161 tcgagcgcac cgggttcatc gaccggatgg gccgcctcgt gcgcagcgcc gcacgcctct
    11221 cgccgggcat ggccatcgcc ctcgtgatga cggcgacgat gctgctctcc gccttcatca
    11281 acaacacgcc cgtcgtcgtc atcctcatcc cgatcgtcgt cagcctcgcc cgcaccatgg
    11341 ggatctcgcc ggccaagctg ctgatgccgg tctcctacgc cgcgatcttc ggcggcacga
    11401 cgacgctgat cggcacctcc accaacatcc tggtcgacgg cgtcgcccag gcccgcggcc
    11461 tcgccccctt cggcatgttc gagatcaccc tggccggcct ggcgctgggc gtggtcggca
    11521 tcggctacat ggtgaccatc ggccgccatc tcctgcccga ccgcgacagc ctcgccgcgc
    11581 tcctgcccga cggcagccag cggcgctttc tcgtcgacgc gctgatcccg caggactcgc
    11641 cgcatgtcgg caagagcctc gccgatgccg gcttccagcg cgagcgcggc taccgggtga
    11701 tcgacgtcat ccgcggcgac cagtcgctgc gccgcgacct cgacgccgtg ctgctcgccg
    11761 gcggcgaccg cctcgtgctg cgcagccgga tcgccgacgt gctggcgctc aaggagacgg
    11821 gccacgtcgc cttcggcggc gagggcgaga acgcggtcga gccgatcggc agccgcgaga
    11881 ccgtgctggt cgagggcatc gtcgggccgg gctcgggctt caccggcagg cgcatcgccg
    11941 acctgatgct gcgccgccgc tacggcgtct atccgctcgc catgcaccgc cagggcgaga
    12001 acgtccccgg ccgcttcgac gaggtcaaga tccggctcgg cgacacgatc ctgctcgagg
    12061 gcgcggccca gggcatcaag atgctggtcg acgaggggca gatcatcaac ctgatccaga
    12121 ccgacgcgcg gccggcgcgc cgcgaccggg catggatcgc gatcggcgcc atcgtgctgg
    12181 tcatgctgct cgccgccttc gaggtcatgc cgatcgccgg cctggcgctc atcgccgcca
    12241 tcgccgtggt cgccaccggc tgcgtcgacg ccgacgaggc ctatggcgcc atccgctgga
    12301 acatcctgat gctgatcttc gccatgctgg cgctcggcat cgccatggac aagaccggct
    12361 cggtggcgct ggtggtcgac aacctcgtcc tcctgctcgg cggcctcggc ccctgggcga
    12421 tgctcgccgc cctctacttc atgacctcgg cgatcaccga gatcgtcagc aacaacgcca
    12481 ccgccatcct catggcgccg atcgccatcg gcctcgccga caccctcggc gtcgatgccc
    12541 gccccttcgt cgtggcggtc atgctcgccg cctcggcgag cttcgccacg ccgatcggct
    12601 accagaccaa cctgctcgtc tacaatgcgg gcggctaccg cttcggcgac ttcctcaagg
    12661 tgggcctgcc gctcaacctc ctgatgtggc tgaccgcctg cctcgtcatc ccgctgctct
    12721 ggccgctctc ctgagaggtc aggaccggcc gccgtcctgc atccgccgcc gcgcatagac
    12781 cgtggcccgc cgctgtgccc gggcgacgaa acgctcggcc gcggggccgg tgggctcgcc
    12841 gtcatagagc ccggcttggc gcagcgagtt gaaatagccg gcgcccggca ggccgtcgct
    12901 cgcccgcacc acgaggcagg caaggtccgg tccgcccgag gcctcgatcc ggtcgcagac
    12961 ccggccgaga tccttgcaga gggcggccac catcaccggc gtcacccgcc gctcgaagaa
    13021 ggccaggagc gagccgtagg tctccgcccg tccggcgagc gccgcccgga gcagccgggc
    13081 ttcgagcaga tcgagatcga ccacggccgg gcgctctccc ttatgcagct gtggcctcga
    13141 tagctagcgt ttcaccccgg caagggctac attgcgatgg ctcatcggag ggtttctctt
    13201 gcgccgccta ctcgtgctcg tctcgctgct cgtcgccgtg tccgccacgg cggggccgct
    13261 cgccgcgacc acgccgctcg gcatcttcgg cgatgacgac cagcacatca tcgacgccgc
    13321 cgaccggcgc tggaatgccg tgggccgcct caaccgcgaa tccggcggct tctgcaccgc
    13381 cgtgctgatc ggcccaaagg aag
//
LOCUS       DAOTFI010000136        13298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5574_length_13298_cov_6.459639, whole genome shotgun sequence.
ACCESSION   DAOTFI010000136 DAOTFI010000000
VERSION     DAOTFI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5574_length_13298_cov_6.459639"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="P5558_14360"
     CDS             complement(<1..838)
                     /locus_tag="P5558_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY25545.1"
                     /translation="MAGEPLLACRGIAKAFGGVQALRGVDFDVHAGRVHALVGENGAG
                     KSTLTKIIAGLYPADAGTMHLAGEPLAVTDTAAALGRGIVTVHQDVNLILTQTVAENV
                     FLANEPTTGPFGIIRGRELRRRTAELLDRYAIACTPDTLVAELPNDQRKMVQILKAIS
                     REARLLLLDEPTSSLTETEVRMVLRLIRELAGQGVGIVFISHYLTEVFEVADAITVLR
                     DGALALAGERADLTLPDVVAAMIGRELDHQHERGAGRVIGAPLLEVAGLSVRGGPRDV
                     AFT"
     gene            complement(868..1827)
                     /locus_tag="P5558_14365"
     CDS             complement(868..1827)
                     /locus_tag="P5558_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY25546.1"
                     /translation="MHNRRGMITGAILAMGLLAGAPALAEDYTVGYSQFWGTNPFLVT
                     MANGAKKAAAEWAEKGVNVDIIFTNGGDTDTTRQVADLEDLYAQGIEGLILFPGDSIV
                     VAEPVKNIYNPEGIPVVITDIGLQSGTWDTFIITDNYAGGQAGADLMAANVPAGAKVI
                     TFDHAPGNDNAQNRQRGFEDRAKELGLDVQEEKFLKLSLEEGRRLMEDTLVAIPDIKG
                     VFFFNQVVAQGAMAALEAAGRDDVKLVSFDLDPVSFELVKEGRILGLVVQDPFLMGYE
                     GVNAMVTRLQGGTPVERMDIPTRILTQENAAEFADDPQVTGGQ"
     gene            2057..3394
                     /locus_tag="P5558_14370"
     CDS             2057..3394
                     /locus_tag="P5558_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25547.1"
                     /translation="MKTLAIGLLAGTFLTGIAVQSASAEDLVIWWNKSYYQEEDDKFD
                     EIVKAFEAETGINVDYSLFINEDAPVKALAALSAGEVPDLAFGFLFDLQHTSRWAYEG
                     KLVDVSDVLEPMKDRFMPVALESVNLLNGETGERSYYAFPTQQQTAHIHVWNSLLKEI
                     GQSVDDIPGTWNEFWQYYCDQQGELRQVTGNRNFYGVGQPMSSSASDTIFHFMMFANA
                     FDVEWIDDQGNAVLRDHRQQLIDALDSYVMPVRERCNPPGAVNWIDSDNNVNFLNQVT
                     LMTPNPSMSIPASQYSQNPENYTTNIASIEWPDKPGGEPIVYMTSIKTAVIFEGARNV
                     EGAKEFMRFLYEPENIGPYLEGSLGRWFPVMPALVDTPFWQDGTDPHREIHRLQYMER
                     PQNPFPFVYNHRFMPIMTENAIGKAVGRIVLEDWSTEDAADELIERVETALQG"
     gene            3483..4370
                     /locus_tag="P5558_14375"
     CDS             3483..4370
                     /locus_tag="P5558_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25548.1"
                     /translation="MAAHAEKTAAAVAPERNAAPWTPELTWGAILLIPYITVFFLFVV
                     WPVGYGVWLGRDPDSYRRLLADPVYLRTVWNTAAFLGIGVNLKLFLALILSAYFMSQR
                     PWIRWLSILFILPWAVPSIPTILSFRWMLNSEWGMLNGMLWDFFRIEGPWWLVDPKLA
                     LGSAITVHIWKYLPFWTLILMAGRSAIPTDYYEAARIDGASRMQMFAYVTWPQIRNLY
                     VTSTLLSTIWTLGDFNSIYLLTGGGPNELTHVLATLGVRYAFRLQELDMGVATVMTAL
                     PILVPLVFILIRRLGGRVE"
     gene            4370..5281
                     /locus_tag="P5558_14380"
     CDS             4370..5281
                     /locus_tag="P5558_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25549.1"
                     /translation="MAARSEARVRGTTPRRGLWDLVAHPVRRVLEEGFMAVLGVVILI
                     WTILPIYHMAIVSITPLTDAFAGKFWPERPTLENYQTVLFQEHYFLREFWRQLGNSLF
                     VALATMAIVLFVASLASFAISRLKARYGHVVSNTALLTYLIPMAFVAIPFYRVMSDYG
                     LLNTLWALTFAMATFATPYAIWVFRQYSDTIPFELDEAARVDGATPLQLYWLVYLPLM
                     RPAMIAIGTYALLLAWNEYLYAFLLLHNEERATIPVAMGFFLNTDDAPWSLLMATAII
                     YSLPPAALYYSVRRYMVSGLTAGATKA"
     gene            complement(5171..6067)
                     /locus_tag="P5558_14385"
     CDS             complement(5171..6067)
                     /locus_tag="P5558_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25550.1"
                     /translation="MSRLELSRISRVYQSGAAPMTALDGVNLVLSSGEIVGLVGESGS
                     GKTTLARLAVGLDRPTAGIVALDGRPLTDASGRMQRAVRRRVQMVFQDPLASFNPGRS
                     VGGSIALPLRLHTDLDRRDRRSRVAELMTATGLDPSLAGRLPRALSGGQLQRAAIARA
                     LATEPDLLVCDEPVASLDVSVRAQVLNLLVRLKRERGLGVLFVTHDLGVVQRLADRTV
                     VLWRGRVVEEGQGAELWRNPTHPYTQALAAAVPTGLEPWRARRRSGLGGAGRQPAHHV
                     AADGVIEGGGRQRVDDRGRHQE"
     gene            complement(6064..7047)
                     /locus_tag="P5558_14390"
     CDS             complement(6064..7047)
                     /locus_tag="P5558_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25551.1"
                     /translation="MAPVLILERLTVAFPLPDGGWLEATREVDLSLERGRVLALIGES
                     GSGKTTVLNAMAGLLPAEARVTGSLGLDGGGSDLLAPGATREGVAGRRIGMIFQNPGA
                     SLNPVLRVGATVAEVAAVHQGLGRTAAWRAAVELLDRVGIDDPARRASDYPHRLSGGQ
                     KQRVAIAAALAGKPGLILADEPTTALDAMVQAQILDLLLALVDQDGVGLVLVTHDLAV
                     AGAVADEIAVMYAGRIVEQGPAEAVVRRPLHPYAVALAGAALPFGGVAATGDAPFPEL
                     PPADPALPVTACAFAPRCPLAIDRCRSVRPPLEPQDRGHAAACWRAGEAAP"
     gene            complement(7047..7862)
                     /locus_tag="P5558_14395"
     CDS             complement(7047..7862)
                     /locus_tag="P5558_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25552.1"
                     /translation="MRMGSPWSGLIGPALLLLLVLAALLAPLLAPGDPLDMVARPLTA
                     PFTDAWFPLGTDQLGRDVWAGLVHGARVSLVVGLAAAAATLGVGILAGTVAGFRGGLV
                     DDGLMRLTEAFQTVPTFLLALALVSVLGASAGNVVLAIAISSWPPAARLVRAEVLRVK
                     TLDYVDAARIAGMAPLAIAFVEVLPGALQPVVALAGITVGEAILVESALAFLGLGDPN
                     LQSWGSMVANGRALIRTAPSLVILPGLCIAMAVLAVSLTGDGLARRLGLGTRS"
     gene            complement(7862..8857)
                     /locus_tag="P5558_14400"
     CDS             complement(7862..8857)
                     /locus_tag="P5558_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25553.1"
                     /translation="MSWRRLGRRLLQAVPVLLLVAIGVFVLLELAQGDAVDAYLAGIG
                     GAGDLTLAAELRQRYGLADSLGARFLTYATSLATLDLGWSIAFARPVLDVILERLPKT
                     LLLMGSGILLAALLGIGLGALAALRRGRPTDTVVTTAALVLNATPGFWLGLLLIIVFS
                     VELRWLPLGGFSTLDSRATGLEAVLDTARHLVLPAATLGLTYFALYLRLMRGAMLAAA
                     ESGWVTAAKARGLPTRRIVLRHMARPALLPVVTMLGLQSGTMLGGSVVVETVFAIPGL
                     GSLAFEAVSQRDLPLLAGILLAGTVLVVLVNILVDIVYGRLDPRVGDNLGRVAGD"
     gene            complement(8883..10484)
                     /locus_tag="P5558_14405"
     CDS             complement(8883..10484)
                     /locus_tag="P5558_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25554.1"
                     /translation="MGASRREILLSTTALAGVALVARGRGAWAQEPVKGGTLIVAADT
                     EPRNLNPAMVASNGVFYVSSKVVEPLVEMDYETGLRPVLATEWGGSEDGLTYTVKLRE
                     GVTFSDGTPFTSEDVAFSAMQVWKPLQNLGRVVFPNLEAVDTPDPLTAVFTFSVPTPP
                     QLIENALPALSSVLPKHLYDGSDIAENPLNMAPVGTGPFVFAEHRPGEFIRLERNPTY
                     WGEGQPYLDAILYRVLPDAGAKAAALETGDIHLTAFSAVPLAEMPRLDALDDVTVVDK
                     GYEGITYQITLEINHRRAELANPLVRQALRHAIDPQFIVDVVFMGYATAATGPVPSSA
                     ADFYTADVATYDFDIAAAEALLDEAGYPRGADGTRFSLRLRPAPWFEQTRAVGDYVRQ
                     ALQKVGIAVELVTADPGGHIAAVYTDHDFDLAIGSPVYRNDPAISTTILFQGGLPAGV
                     PFSNQYGYDDPAMNEIIAKGLRTIDSAERVAVYEEFQRKAAEDLPILVLVDFTFLTVA
                     RDEVRNVANNPRWATSSWADTWLQP"
     gene            complement(10605..13004)
                     /locus_tag="P5558_14410"
     CDS             complement(10605..13004)
                     /locus_tag="P5558_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY25555.1"
                     /translation="MAPSDPSRAAREKLMGQPVRRKEDQRFVTGTGRYVADIVLPRET
                     VGVVVRSDHAHAELRAVHTEAAVAMPGVLAVYTGKDLDAAGIGTLPCGWMVQSRDGSP
                     MAQPPHPVLALGRVRHVGDPVAFVVAETRAEALAAAEAVTVDYAPLPNVTDLATAVTP
                     EAPLLWPEAPGNLCYDWEVGDEAAVEAAFRAAHHVTTVDLVNNRINASPMETRGTIGD
                     WDAGTERLTLYTSNQNPHIIRLLMALATLKLSEEKIRVVSPDVGGGFGMKIYHYAEEV
                     LVGFAAMRLRRPVKWIAERSEAYLSDTWARDHVTTASLALDASGVVTGLRVDTLANMG
                     AYLSTFATAIPSFFYANPLPGPYAVRAVHCRVRAVFTNTVPVDAYRGAGRPEATYVLE
                     RLMDAAARETGLDRVEIRRRNLVPADAFPYKTPLLWTYDVGDFQTLLDRALEVADQAG
                     FAARREASEAAGLRRGFGFAFYMEACGMGPSKMLKEHGCAAGQYEVGHVRVNPTGTVT
                     VLTGSHTHGQGHETVYAQIVADRLGVPFERVEIVHGDTDRVAYGIGTYGSRSLALGGS
                     ALLMSLDKVVEKGRKIAAHLLEADVGDVSFADGRFSVAGTDLEVDFAAVVTAAYNPWN
                     YPLDELEPGLEETSYFDPPDFTFPYGCHLCEVEVDPLTGRVRVDRFTAVDDFGHQINP
                     MIVAGQLHGGVAQGIGQALLELGVHDGETGQLLSGSFMDYAMPRASDVPSVEVASLAI
                     PCRNNPLGVKGCGEAGAIAGPAAVVSAVLDALEPSGVTRLDMPLTPERVWRGLRQAAA
                     L"
     gene            complement(12991..>13298)
                     /locus_tag="P5558_14415"
     CDS             complement(12991..>13298)
                     /locus_tag="P5558_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRY25556.1"
                     /translation="TAVVVGDAIASPTTTAVKVPDGIDGAALLATSRERYGVTFSAGR
                     AETLGKLIRIGHMGPTAQPIHSIAAVAALGAALQAMGGRADIGGGTEAAMEIINGAF"
BASE COUNT         2068 a         4598 c         4712 g         1920 t
ORIGIN      
        1 gggtgaaggc gacgtcgcgc gggccgccgc gcaccgagag cccggccacc tcgagcagcg
       61 gcgcgccaat aacccggccg gcgccgcgct cgtgctggtg atccagctcg cggccgatca
      121 tggcggccac cacgtcgggc aaggtgaggt cggcccgctc gccggccagg gcaagggcgc
      181 catcgcggag cacggtgatc gcgtcggcga cctcgaagac ctcggtcagg tagtgggaga
      241 tgaagacgat gccgaccccc tgcccggcca gctccctgat cagcctgagg accatgcgga
      301 cctcggtctc ggtgagcgag gaggtcggct cgtcgagcag gaggaggcgc gcctcgcggc
      361 tgatggcctt gaggatctgg accatcttgc gctggtcgtt gggcagctcc gccaccagcg
      421 tgtcgggcgt gcaggcgatg gcgtagcggt cgagcagctc ggccgtgcgc cggcgcagct
      481 cacggccgcg gatgatgccg aagggccccg tcgtcggctc gttggcgaga aagacgttct
      541 cggccacggt ctgcgtcagg atcaggttga cgtcctggtg gacggtgacg atgccgcgac
      601 cgagcgccgc cgccgtgtcg gtgacggcca agggctcgcc ggcgaggtgc atcgtgccgg
      661 catcggcggg gtagaggccg gcgatgatct tggtcagcgt cgacttgccg gcgccgttct
      721 cgccgacgag cgcatgcacg cgcccggcat ggacgtcgaa atcgacgccc ctgagcgcct
      781 gcaccccgcc gaaggccttg gcgatgcccc ggcaggcgag cagcggctcc cccgccatcg
      841 gccgattcga tccgccagga acgacggcta ctggccgccc gtcacctgcg ggtcgtcggc
      901 gaactcggcc gcgttctcct gggtcaggat cctcgtcggg atgtccatcc gctcgaccgg
      961 cgtgccgccc tgcagcctgg tcaccatggc gttgacaccc tcgtagccca tgaggaaggg
     1021 gtcctggacg acgaggccga ggatccggcc ctccttgacc agctcgaagc tgaccgggtc
     1081 gaggtcgaag gagacgagct tgacgtcgtc gcgaccggcc gcctcgagcg ccgccatcgc
     1141 accctgcgcc accacctgat tgaagaagaa gacgcccttg atgtcgggga tggcgaccag
     1201 cgtgtcctcc atcagccgcc gtccctcctc gagcgagagc ttgaggaact tctcctcctg
     1261 gacgtcgagg ccgagctcct tcgcccggtc ctcgaagccg cgctggcggt tctgggcgtt
     1321 gtcgttgccg ggggcgtggt cgaaggtgat gaccttggcc cctgccggca cgttcgccgc
     1381 catgaggtcg gcgccggcct gcccgccggc atagttgtcg gtgatgatga aggtgtccca
     1441 ggtgccggac tgcaggccga tatcggtgat gacgaccggg atgccctcgg ggttgtagat
     1501 gttcttgacc ggctcggcga cgacgatgct gtcgcccggg aagaggatca ggccctcgat
     1561 cccctgggcg tagagatcct cgaggtcggc cacctggcgg gtggtgtcgg tgtcgccgcc
     1621 attggtgaag atgatgtcga cattgacacc cttctcggcc cactcggcgg cggccttctt
     1681 cgccccgttc gccatggtga cgaggaaggg gttggtgccc cagaactgcg agtagccgac
     1741 ggtgtagtcc tcggccaggg cgggtgcgcc cgccagcagg cccatggcca ggattgcccc
     1801 ggtgatcatc ccgcgccgat tgtgcatcgt cgttcccccc cttggccgaa gtcgcccgat
     1861 cgcttcgtgc ggggcggagc ttgccacgcg gccgatgccg agacaaggca tgccgcggcg
     1921 cccgcctcac gggttttgac aaagcccggc cgacgcccct actcttcggc tgccctcggc
     1981 gggcttggcg aggcaagaag accgatcgac cgttcaacga gtcgactcaa ctcagggatc
     2041 agggagcgcg aagagcatga aaacactggc tatcggcctg ttggccggca ccttcctcac
     2101 cggcatcgcc gtccaatcgg cgtcggccga ggacctcgtg atctggtgga acaagagcta
     2161 ctaccaggag gaggacgaca agttcgacga gatcgtcaag gcgttcgagg ccgagacggg
     2221 catcaatgtc gactacagcc tgttcatcaa cgaggacgcg ccggtcaagg cgctggcggc
     2281 gctctcggcc ggcgaggtgc cggatctcgc cttcggcttc ctcttcgacc tgcagcacac
     2341 ctcgcgctgg gcctacgagg gcaagctggt cgacgtcagc gacgtgctgg agccgatgaa
     2401 ggaccggttc atgccggtgg cgctggaatc ggtgaacctg ctcaacggcg agaccggcga
     2461 gcggtcctac tacgccttcc cgacccagca gcagaccgcc cacatccacg tctggaacag
     2521 cctgctgaag gagatcggcc agagcgtcga cgacattccc ggcacctgga acgagttctg
     2581 gcagtattac tgcgaccagc agggcgagct ccggcaggtc acgggcaacc gcaatttcta
     2641 tggcgtcggc cagcccatgt cgagctcggc ctcggacacc atcttccatt tcatgatgtt
     2701 cgccaacgcc ttcgacgtcg aatggatcga cgaccagggc aacgccgttc tccgcgacca
     2761 tcgccagcag ctgatcgacg cgctcgacag ctacgtcatg ccggtcaggg agcgctgcaa
     2821 cccgccgggt gcggtgaact ggatcgacag cgacaacaac gtcaacttcc tcaaccaggt
     2881 gacgttgatg acgcccaacc cgtccatgtc gatcccggcc tcgcagtact cgcagaaccc
     2941 ggagaactac acgaccaaca tcgccagcat cgagtggccg gacaagcccg gcggcgagcc
     3001 catcgtctac atgacgtcga tcaagacggc ggtgatcttc gagggggcca ggaacgtcga
     3061 gggggcgaag gagttcatgc gcttcctgta cgagccggag aacatcggcc cctatctcga
     3121 gggctcgctc ggccgctggt tcccggtgat gcccgcgctg gtcgacacgc cgttctggca
     3181 ggacggcacc gatccgcacc gggagatcca ccggctgcag tacatggagc ggccccagaa
     3241 cccgttcccg ttcgtctaca accaccgctt catgccgatc atgaccgaga acgccatcgg
     3301 caaggcggtc gggcgaatcg tgctggagga ctggtcgacg gaggatgcgg cggacgagct
     3361 gatcgagcgc gtcgagacgg cgctccaggg ctgacgcctg atctgggggc ggcgccgcag
     3421 gagcggcgcc ccctcgcttc ccgagcccgc gtcgcagcgg gccgtggctg ggagatggcg
     3481 ggatggcggc acatgccgag aagaccgcag cggcagtcgc accggagcgc aacgccgcgc
     3541 cctggacccc ggagctcacc tggggcgcca tcctgctcat cccctacatc accgtcttct
     3601 tcctcttcgt ggtctggccg gtcggctacg gcgtctggct cggccgggat cccgacagct
     3661 atcggcggct gctggccgac ccggtctacc tgcgcacggt ctggaacacc gccgccttcc
     3721 tcggcatcgg cgtcaacctc aagctcttcc tggcgctgat cctctccgcc tacttcatgt
     3781 cgcagcggcc ctggatccgc tggctctcga tcctcttcat cctgccctgg gcggtgccgt
     3841 cgatcccgac catcctctcc ttccgctgga tgctgaactc cgagtggggg atgctgaacg
     3901 gcatgctctg ggacttcttc cggatcgagg gcccctggtg gctggtcgac cccaagctgg
     3961 cgctgggctc ggcgatcacc gtccacatct ggaagtacct gccgttctgg acgctgatcc
     4021 tgatggccgg gcggagcgcc attccgaccg actactacga ggcggcgcgg atcgacggcg
     4081 ccagccgcat gcagatgttc gcctacgtca cctggccgca gatccgcaat ctctacgtca
     4141 ccagcacgct gctctcgacc atctggacgc tcggcgactt caacagcatc tacctcctga
     4201 ccggcggcgg gccgaacgag ctcacccatg tgctggcgac gctcggcgtg cgctacgcct
     4261 tccgcctgca ggaactcgac atgggtgtcg ccaccgtgat gacggccctg ccgatcctgg
     4321 tgccgctcgt cttcatcctg atcaggcggc tcggcgggag ggtcgagtga tggcggcccg
     4381 gagcgaggcc agggtgcggg gcaccacgcc gcggcgtggc ctctgggacc tcgtcgccca
     4441 tccggtgcgc cgggtgctcg aggaaggctt catggccgtc ctcggcgtcg tcatcctgat
     4501 ctggacgatc ctgccgatct accacatggc catcgtctcg atcacgccct tgaccgacgc
     4561 cttcgccggc aagttctggc cggagcggcc gacgctcgag aactaccaga ccgtgctctt
     4621 ccaggagcac tacttcctgc gcgagttctg gcggcagctc ggcaacagcc tcttcgtggc
     4681 gctcgccacc atggccatcg tcctcttcgt cgccagcctc gcgagcttcg ccatcagccg
     4741 gctcaaggcc cgctacggcc atgtggtctc gaacacggcc cttcttacct acctcatccc
     4801 catggccttc gtcgccatcc cgttctaccg ggtgatgtcg gattacggcc tcctgaacac
     4861 gctctgggcc ctcaccttcg ccatggcgac cttcgccacg ccctacgcga tctgggtctt
     4921 tcgccagtac agcgacacca tccccttcga gctcgacgag gcggcccggg tcgacggcgc
     4981 gacgccgctc cagctctact ggctggtcta cctgccgctc atgcggccgg cgatgatcgc
     5041 catcggcacc tatgcgctgc tgctcgcctg gaacgagtat ctctatgcct tcctcctgct
     5101 gcacaacgag gagcgggcaa ccatcccggt cgccatgggc ttcttcctca acaccgacga
     5161 cgcgccctgg tcactcttga tggcgaccgc gatcatctac tcgctgccgc ccgccgccct
     5221 ctattactcc gtccgccgct acatggtgag cgggctgacg gccggcgcca ccaaggcctg
     5281 accgccggcg cgcccgccag ggctcgagcc cggtcggcac cgccgccgcc agggcctgcg
     5341 tgtaggggtg ggtggggttc cgccagagct ccgccccctg cccctcctcg accaccctgc
     5401 cccgccagag aacgaccgtg cgatcggcga ggcgctgcac cacgccgagg tcatgggtga
     5461 cgaagagcac gccgagcccg cgctcccgct tgaggcggac gaggagattg agaacctggg
     5521 cgcggaccga gacgtcgagc gaggccaccg gctcgtcgca gaccagcagg tccggctcgg
     5581 tggcgagcgc ccgggcgata gccgcccgct ggagctggcc acccgaaagc gcccggggca
     5641 ggcggccggc cagcgaagga tcgaggccgg tggcggtcat cagctcggcg acccgcgatc
     5701 gccgatcccg gcggtcgagg tcggtgtgca ggcgaagcgg cagggcgatc gagccgccga
     5761 cgctgcggcc cgggttgaag ctggcgagcg ggtcctggaa gaccatctgc acgcgccggc
     5821 gaacggcccg ctgcatccgc ccgcttgcat cggtcaaggg ccggccgtcg agcgcgacga
     5881 tgcccgcggt cggccggtcg agcccgaccg cgagccgggc gagcgtcgtc ttgccggacc
     5941 cgctctcgcc gacgaggccg acgatctcgc ccgacgagag cacgaggttg actccgtcga
     6001 gggccgtcat cggcgccgcg ccgctctggt agacgcggct gatgcgcgag agctcgagcc
     6061 ggctcaaggg gctgcctccc cggcccgcca gcaggcggcg gcatggcccc tgtcctgcgg
     6121 ctcgagcggc gggcgcacgc tccggcaacg atcaatggcg agcgggcagc gcggcgcgaa
     6181 ggcgcaagca gtgacaggca gcgccggatc ggcgggaggc agctcgggaa agggcgcgtc
     6241 gccagtggcg gccacaccgc cgaacggcag ggcagcgccg gcgagggcca cggcataggg
     6301 atggagcggg cggcggacca cggcctccgc gggcccctgc tcgacgatcc ggccggcata
     6361 catgacggcg atctcgtcgg cgacggcccc ggccacggcg aggtcgtggg tcacgaggac
     6421 gaggccgact ccgtcctggt cgaccagcgc cagcagcagg tcgaggatct gcgcctggac
     6481 catggcatcg agcgcggtgg tcggctcgtc ggcgaggatc aggcccggct tcccggcaag
     6541 ggcggcggcg atcgccacgc gctgcttctg cccacccgac agccggtgcg ggtagtccga
     6601 tgctcgccgc gccgggtcgt cgatgccgac ccggtcgaga agctcgacgg cggcccgcca
     6661 ggcggcggtg cggcccaggc cctgatgcac ggcggcgacc tcggcgacgg tggcgccgac
     6721 ccgcagcacc gggttgaggc tcgcacccgg gttctggaag atcatgccga tccgccggcc
     6781 ggcgacgccc tcgcgcgtcg caccgggcgc gaggagatcg gagccgccgc catccagccc
     6841 gaggctgccc gtgacccgtg cttccgccgg caggagtccc gccatggcgt tcagcacggt
     6901 cgtcttgccg gagccgctct cgccgatgag ggcaaggacc cggccccgct cgagcgaaag
     6961 gtcgacctcc cgggtggcct cgagccagcc gccatccggc agcgggaagg ccacggtcag
     7021 ccgctccaga atgaggaccg gcgccatcag cttcgcgtgc cgaggccgag gcggcgggcc
     7081 aggccgtcgc cggtgaggct gaccgcgagc acggccatgg cgatgcagag cccgggcagg
     7141 atgaccagcg agggcgccgt gcggatcagg gcccggccat tcgccaccat cgagccccag
     7201 ctctgcaaat tggggtcgcc gaggccgaga aaggccaagg cgctctccac caggatggct
     7261 tcgcccacgg tgatgccggc cagcgccacg accggctgca gggcaccggg caggacctcg
     7321 acaaaggcga tggccaaggg cgccatgccg gcgatgcgcg ccgcatcgac atagtccagc
     7381 gtcttcacgc gcagcacctc ggcgcgcacg aggcgggccg ccggcggcca ggacgagatg
     7441 gcgatggcca gcaccacatt gccggccgag gccccgagca cgctgaccag cgccagcgcc
     7501 aggaggaagg tcggcacggt ctggaaggcc tcggtcaggc gcatcagccc gtcgtcgacg
     7561 aggccgcccc gaaagcccgc gacggtgccg gccaggatgc cgacgccgag cgtggcggcg
     7621 gcggcagcca gcccgacgac gagcgagacc cgggcgccgt ggacgagccc ggcccagacg
     7681 tcgcggccga gctgatccgt gccgagcggg aaccacgcgt cggtgaaggg tgcggtcagc
     7741 ggccgggcca ccatgtcgag cggatcgccc ggggcgagca gtggcgcgag cagtgccgcc
     7801 agcaccagca gcagcaggag ggccgggccg atcaggccgc tccagggcga ccccatgcgc
     7861 atcagtcgcc cgccacccgg ccgaggttgt cgccgacccg cgggtcgagc cggccataga
     7921 cgatatcgac caggatgttg accagcacga cgaggaccgt cccggcgagc aggatgccgg
     7981 ccagcagcgg caggtcgcgc tggctcaccg cctcgaaggc gaggctgccg aggcccggga
     8041 tcgcgaagac cgtctcgacg acgacgctgc cgccgagcat cgtgccggac tgcaggccga
     8101 gcatggtgac gaccggcaga agcgccggcc gcgccatgtg gcgaaggacg atgcggcggg
     8161 tgggcaggcc ccgggccttg gccgcggtca cccagccgct ttcggctgca gccagcatcg
     8221 cgccgcgcat caggcggaga tagagggcga aataggtgag ccccagcgtg gccgccggca
     8281 ggaccaggtg gcgggcggtg tcgagcacgg cctcgaggcc ggtcgcccgg ctgtcgagcg
     8341 tgctgaagcc gcccaagggc agccagcgca gctcgaccga aaagacgatg atcaggagca
     8401 ggccgagcca gaagccgggc gtggcgttga ggaccagggc cgccgtcgtg accaccgtgt
     8461 ccgtcggccg gccgcgacgg agggcggcca gggcgccgag gccgatgccg agcagggccg
     8521 cgagcaggat gcccgagccc atgagcagga gcgtcttggg caggcgctcg aggatgacgt
     8581 cgagcaccgg ccgggcgaag gcgatcgacc agccgagatc gagggtggcg aggctcgtgg
     8641 cataggtgag gaaacgcgcg cccaggctgt cggcgaggcc gtagcgctgg cgcagctcgg
     8701 cggcgagcgt gaggtcgccc gcaccgccga tgccggcgag ataggcatcg acggcgtcac
     8761 cctgggcgag ctcgagcagg acgaagacgc cgatggcgac gagcaggagc accggcaccg
     8821 cctgcagcag gcggcggccg agacggcgcc aggacatcag cttgctgtca ggctggccag
     8881 cctcagggct ggagccaggt gtcggcccag gacgaggtgg cccagcgcgg gttgttggcg
     8941 acgttccgca cctcgtcgcg cgccacggtg aggaaggtga agtcgaccag cacgaggatc
     9001 ggcaggtcct cggcagcctt tcgctggaat tcctcgtaga cggcgacccg ttccgcgctg
     9061 tcgatggtcc tgagcccctt ggcgatgatc tcgttcatcg ccgggtcgtc gtagccgtac
     9121 tggttcgaga acggcacgcc ggccggcagg ccaccctgga agaggatggt ggtcgagatg
     9181 gccgggtcgt tgcgatagac cggcgagccg atggcgaggt cgaagtcgtg gtcggtgtag
     9241 acggcggcga tgtggccgcc cgggtcggcc gtgaccagct ccaccgcgat cccgaccttc
     9301 tgcagggcct ggcggacgta gtcgccgacc gcccgggtct gctcgaacca gggtgccggc
     9361 cggagcctga gcgagaagcg cgtgccgtcg gcgccgcgcg ggtagccggc ctcgtcgagc
     9421 agcgcctcgg cggcggcgat gtcgaagtcg taggtcgcca cgtcggccgt gtagaaatcg
     9481 gcagcgctcg acggcaccgg gccggtggcg gcggtggcgt agcccatgaa gaccacgtcg
     9541 acgatgaact ggggatcgat ggcgtggcga agggcctgcc ggaccagcgg gttggccagc
     9601 tccgcgcggc ggtggttgat ctccagcgtg atctggtagg tgatgccctc gtagcccttg
     9661 tccacgaccg tgacgtcgtc gagcgcatcg agcctcggca tctcggcgag cggcacggcg
     9721 gagaaggcgg tgaggtggat gtcacccgtc tccagggccg ccgccttcgc cccggcatcc
     9781 ggcagcacgc ggtagaggat ggcatcgaga tagggctggc cctcgcccca gtaggtgggg
     9841 ttccgctcga gccggatgaa ctcgcccggc cggtgctcgg cgaagacgaa gggccccgtg
     9901 ccgaccgggg ccatgttgag cgggttctcc gcgatgtccg agccgtcgta gagatgcttc
     9961 ggcaggaccg aggaaagggc cggcagcgcg ttctcgatga gctgcggcgg cgtcggcacg
    10021 gagaaggtga agacggcggt gagcgggtcg ggcgtgtcga ccgcctcgag gttcgggaag
    10081 accacccggc cgaggttctg caggggcttc cagacctgca tcgccgagaa cgcgacatcc
    10141 tcggaggtga agggcgtgcc gtcgctgaag gtgacgccct cgcgcagctt caccgtgtag
    10201 gtcagcccgt cctcggagcc cccccattcc gtcgccagca ccgggcgcag ccccgtctcg
    10261 tagtccatct cgacgagcgg ctcgaccacc ttgctcgaca cgtagaagac gccgttggac
    10321 gccaccatgg ccgggttgag gttgcgcggc tcggtgtcgg cggcgacgat cagcgtgcca
    10381 cctttcacgg gctcctgggc ccatgcgccg cgcccgcgcg cgacgagcgc cacgccggcc
    10441 agggccgtcg tcgaaagcag gatctcgcgc cgcgaagcac ccatcaccat tttccccctt
    10501 ctgtggttgc cacgacttta ggagtgttct cgccgcggcg catcagcgga ccgcgccggc
    10561 atcgacgtcg atcgtccggc cggccgctcg tcatgtccgc cgagtcacaa cgctgccgcc
    10621 tgccgcagcc cgcgccagac gcgctccggc gtcagcggca tgtcgagacg ggtgacgccc
    10681 gatggctcga gcgcgtcgag cacggcgctg accacggcgg ccgggccggc gatggccccg
    10741 gcctcgccgc agcccttcac ccccaagggg ttgttgcggc agggaatggc gagcgacgcg
    10801 acctcgacgg acggcacgtc cgaggcccgc ggcatggcgt agtccatgaa ggagcccgag
    10861 aggagctggc cggtctcccc gtcgtgcacg ccgagctcga ggagcgcctg gccgatgccc
    10921 tgcgccacgc cgccatggag ctggcccgcc acgatcatcg ggttgatctg gtggccgaaa
    10981 tcgtcgaccg cggtgaaccg gtcgacgcgc acccggccgg tgagcgggtc gacctcgacc
    11041 tcgcacaagt ggcagccata ggggaaggtg aagtccggcg ggtcgaagta gctggtctcc
    11101 tcgagcccgg gctcgagctc gtcgagcggg tagttccagg gattgtaggc ggcggtcaca
    11161 accgcggcga agtcgacctc gagatcggtg ccggcgacgc tgaagcggcc atcggcgaag
    11221 ctcacgtcgc ccacgtcggc ctcgagcagg tgggccgcga tcttgcggcc cttctcgacc
    11281 accttgtcga gcgacatcaa gagggccgag ccgcccagcg ccagcgagcg cgagccgtag
    11341 gtgccgatgc cgtaggcgac gcggtcggtg tcgccatgga cgatctcgac ccgctcgaag
    11401 ggcacaccca gccggtcggc gacgatctgc gcatagaccg tctcgtggcc ctggccgtgg
    11461 gtgtggctgc cggtgagcac cgtcaccgtg ccggtcgggt tgacccgaac atggcccacc
    11521 tcgtactggc cggcggcgca gccgtgctcc ttgagcatct tcgacggccc catgccgcag
    11581 gcctccatgt agaaggcgaa gccgaagccg cggcggaggc cagccgcctc gctggcctcc
    11641 cgccgggcag cgaagccggc ctggtcggcg acctcgagcg cccggtcgag cagcgtctgg
    11701 aaatcgccga catcgtaggt ccagagcagc ggcgtcttgt aggggaaggc gtcggcgggc
    11761 acgaggttgc gccggcggat ctccacccgg tccaggcccg tctcgcgcgc cgcggcatcc
    11821 atcaggcgct ccagcacata ggtcgcctcg ggccgtcccg ccccccgata ggcgtcgacc
    11881 ggcacggtgt tggtgaagac cgccctgacc cggcaatgca ccgcgcgcac ggcatagggg
    11941 ccgggcagcg ggttggcgta gaagaagctc gggatggccg tggcgaaggt cgagagatag
    12001 gcccccatgt tggcgagcgt gtcgacccgc agcccggtga cgacgccgct cgcgtcgagg
    12061 gcgagcgacg ccgtggtgac gtggtcgcgg gcccaggtgt cggaaagata ggcctcgctc
    12121 cgctcggcga tccacttgac cgggcgcctg agccgcatcg ccgcgaagcc gaccagcacc
    12181 tcctcggcat agtggtagat cttcatgccg aagccgccgc ccacatcggg cgagacgacc
    12241 cggatcttct cctcggagag cttgagcgtg gccagcgcca tcaggagccg gatgatgtgc
    12301 gggttctggt tcgaggtgta gagcgtcagc cgttcggttc ccgcgtccca gtcgccgatc
    12361 gtgccgcgcg tctccatggg cgaggcgttg atgcggttgt tgacgaggtc gaccgtcgtg
    12421 acgtggtgcg ctgcgcgaaa ggcggcctcg acggcagctt catcgccgac ctcccagtcg
    12481 tagcagagat tgcccggcgc ctcgggccag agcaaaggcg cttcgggcgt cacggcggtc
    12541 gcgagatcgg tgacgttggg caaaggcgcg tagtcgacgg tcacggcctc ggcggcggcc
    12601 agcgcctcgg cccgggtttc agcgaccacg aaagccaccg gatcgcccac atggcggacc
    12661 cgtcccagcg ccagcaccgg gtgcggcggc tgcgccatgg gcgagccgtc cctcgactgc
    12721 accatccagc cgcagggcag cgtgccgatg ccggcggcat cgaggtcctt gccggtatag
    12781 acggcgagca cgcccggcat ggcgacggcg gcttcggtat gcacggcacg cagctcggca
    12841 tgcgcatggt cgctgcgcac cacgacgccg acggtctcgc gcggcagcac gatgtcggcg
    12901 acgtagcggc cggtgccggt gacgaagcgc tggtcctcct tgcggcggac cggctggccc
    12961 atcagcttct cgcgtgccgc ccgggaagga tcagaaggcg ccattgatga tctccatcgc
    13021 agcttccgtg ccgccgccga tatcggccct gccgcccatc gcctgcagcg ccgcgcccaa
    13081 tgccgcgacc gccgcaatgc tgtggatcgg ctgggcggtc gggcccatgt ggccgatgcg
    13141 gatgagcttc cccagggtct cggcccggcc ggcggagaag gtgacaccgt agcgctcgcg
    13201 cgacgtggcg agaagggccg caccgtcgat gccgtccggc accttcacgg cggtggtggt
    13261 gggcgacgcg atcgcgtcgc ccaccaccac cgccgtga
//
LOCUS       DAOTFI010000137        13278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5583_length_13278_cov_6.644105, whole genome shotgun sequence.
ACCESSION   DAOTFI010000137 DAOTFI010000000
VERSION     DAOTFI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13278
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5583_length_13278_cov_6.644105"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /gene="hisE"
                     /locus_tag="P5558_14420"
     CDS             <1..1084
                     /gene="hisE"
                     /locus_tag="P5558_14420"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRY25557.1"
                     /translation="GSNRALIERLVARFDCHVGGGIRDVETALFWLDAGARRIILGTM
                     AKSEILEALPKDRLIAALDARHGEVVVEGWKEGTGRGILERVAELKDLVDGFLVTFVE
                     REGRMGGTDMALARTIVEAAAPARVIIAGGVTTPAEIAELDAMGADAQVGMALYSGRL
                     GLGESVAGIFRSDRPDGLIPTVVTDERGTALGLVYSSQESIAAAVDERRGIYQSRRRG
                     LWRKGESSGDVQELLRVTPDCDRDSVRFTVRQAGAGFCHLATRTCFGEDAGLGRLHRR
                     LLARRSDAPEGSYTARLMAEPTRLAAKVTEEAGELNGAASRDEVVWEAADLLYFTLVR
                     LAAEGIGLDEVERHLDRRERRVRRRD"
     gene            1077..1799
                     /locus_tag="P5558_14425"
     CDS             1077..1799
                     /locus_tag="P5558_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRY25558.1"
                     /translation="MTEDRLHRLIVWLRPLLELRLLLAVLLLGTLAWTFIELAEEVVE
                     GDTRAIDEALLLGLRTAADPAVPIGPEWLQVVMRDITALGGYTILFLVTAATVIYLFL
                     VGRPRTAVFILVAVLGATLLTVLLKLGFARPRPDLVPHAMRTASASFPSGHSSGAAAV
                     YLTLGAILASFQDRRRVKLFIMGLAVVVTMLVGISRLYLGVHWPSDIIAGWALGAGWA
                     VLCWVVAAYLRRARVLHSPPAG"
     gene            complement(1823..3520)
                     /locus_tag="P5558_14430"
     CDS             complement(1823..3520)
                     /locus_tag="P5558_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRY25559.1"
                     /translation="MTTYRAPLGEIGFVIERIAGPPKDAEIDQDDLAAVLAEAGRFAE
                     AELVPLNATGDRPGAMLRDGEVHTAPGFRQAYERFAEGGWMGLVFPEEHGGQGLPWTL
                     NTAVAEIFAAADITFQLCPLLTQGTIEALLRHADRDQQASWLPPLVAGRWTGAMCLTE
                     PQAGSDLAAIRSTAEPADDPVLGPHHRLRGQKVFISWGDHDLTSDLVHLVLARLPDAP
                     SGSKGISLFLVPKRLPDCSRNDWRVLKLEEKLGLHASPTCVVAYGDGPGAVGYLVGAP
                     HGGLAAMFTMMNNARISVGVQGLGLAERAFQGALAYARERRQGQRHGRPAALVDHPDI
                     RRTLLGMRARIAAMRALCLVTAAAVDRAVRDPDLEERRRADLRVALLTPIVKAWCTDG
                     AVAITSDAIQVAGGAGYVEETGLARLFRDARVLPIYEGTNGIQALDLAGRKLRIEDGQ
                     LPWQLFAELEETRLDEALRPTLTQALDRLKQATLHMQGASGEAREAAATPYLDLFGWV
                     LGAHLVAMGAHAAEDDPRGHAWPGLARFYVERLLPPALGLAEVVESAAPLEASWLAG"
     gene            complement(3517..3897)
                     /locus_tag="P5558_14435"
     CDS             complement(3517..3897)
                     /locus_tag="P5558_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25560.1"
                     /translation="MPSTQAALETRLRELGFMLEPDSAPGMIRAGILTGAPAEWFVCD
                     KIRVEDSSGDSSRSRLVDPEDHKTLVQIRVTSLGTQTNVDMKTNYYGVYRDRFDNNLF
                     DQNCVASGALEQAVLAVITPSPPA"
     gene            complement(4011..5465)
                     /locus_tag="P5558_14440"
     CDS             complement(4011..5465)
                     /locus_tag="P5558_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY25561.1"
                     /translation="MTLSALPIENLVAALAPLLGPQGIITEAAGIERYTVDFWHQHKG
                     TTPLVLRPDSTEAVAAIVREAARLGVAIVPQSGNSGLVAGGIPDGSGRQVVLSLERLN
                     RIRAVDAAGGSLVAEAGCILADVQAAADGAGRLFPLSLGAEGSCRIGGNLSTNAGGVN
                     VLRYGMTRQLVLGLEVVLADGRVWNGLRALRKDNTGYDLKQLFIGAEGSLGIITAAVL
                     RLYPRPRERRTIWLGIESPADALRLLSLFQDHLGELISSFELIAGLGVEAANDHLPGG
                     RRPLAEPHPWHLLIEVAWSLDGGLDVPVDRALEAGFDAGLVQDGTIAQNEAQRTAMWL
                     IREGQSEATTKVGHVQRSDISVAIADLPALIARAEAHFGKLAPDVQLFPFGHVGDGNL
                     HFNFLIPRDREGVPELRSLLNEHLMMLVAEFGGSFSAEHGIGRLKRGELERRKDPLEL
                     ELMRRLKATLDPTGILNPGVILAAPDAPASREIS"
     gene            5598..6440
                     /gene="dapD"
                     /locus_tag="P5558_14445"
     CDS             5598..6440
                     /gene="dapD"
                     /locus_tag="P5558_14445"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRY25562.1"
                     /translation="MDVERLKAVIEAGFEDRATVDASTKGELRTAVEAALAGLDAGKF
                     RVAEKGRKGWVVHEWLKKAVLLSFRLNAMERIDGAPGGASWWDKVPSKFAGWRKKDFT
                     EAGFRAVPGCVVRHSAYIAPNVVLMPSFVNLGAYVAEGTMIDTWATVGSCAQIGRNCH
                     ISGGAGIGGVLEPLQANPVIIEDDCFVGARAEVAEGVVVETGSVLSMGVYLGASTKII
                     DRATGEIFAGRVPAYSVVVPGTLPGKPLPDGSPGPGLYCAVIVKRVDARTRSRTSINE
                     LLRD"
     gene            6454..7602
                     /gene="dapE"
                     /locus_tag="P5558_14450"
     CDS             6454..7602
                     /gene="dapE"
                     /locus_tag="P5558_14450"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016737694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRY25563.1"
                     /translation="MSSVDPVELTRALVRCPSVTPADAGAQAVLAEVLEGLGFRVELL
                     RLGDGGEPAIDNLFARLGDGSPHLAFNGHTDVVPAGDDAAWSAGPFAAEIRDGWLYGR
                     GAADMKGDIACFVAAIARYLERHGSVPGSLSLLITGDEEGPAVNGTVALLRWAEAAGH
                     RFDACLVGEPTSVDELGDMAKIGRRGSLTVTLEARGRQGHVGYPHRADNAAHRLVRVL
                     ARLVETPLDEGTELFEPSSLQVTTIDIGNPASNVVPARARAVLNIRYNDSQSPASLKA
                     LVAAAIEAAGGRVEAACTEGANVFACPPGPLAALLGDSVEAVTGRRPVLSTSGGTSDA
                     RFIHSHCPVIEFGLIGQTIHQVDERVPLVELQALTAIYLEFLERYFGQ"
     gene            7599..8168
                     /locus_tag="P5558_14455"
     CDS             7599..8168
                     /locus_tag="P5558_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25564.1"
                     /translation="MMPPFPEVRHYAEGAWRLARGDAGGMGHFDCTVDGYWRSFWAAF
                     YVLPAYALLVADQHARAHRDNGFLSIVVGEGLVYVLGWLVMPVLAIFLTRFFGLQRHY
                     VPLIVALNWASLVQIGILLVPLALGVLLAPSLAALLMLLASGAVLVYKTFVARVALDT
                     TIGTAASFIAAELVIMLMVTGWLYGVFFD"
     gene            complement(8147..8929)
                     /gene="truA"
                     /locus_tag="P5558_14460"
     CDS             complement(8147..8929)
                     /gene="truA"
                     /locus_tag="P5558_14460"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRY25565.1"
                     /translation="MPRYRLLLEFDGGPFCGWQIQANGPSVQAALEAAADRMVRRMGP
                     AVAAGRTDAGVHALALPVQLDLEQTIMPERLMSGLNHHLRHLPIVVLWASLAQDGFHV
                     RFNATARHYRYRILARRAPPALEHGRVWHVARSLDAERMQAAAETLVGRHDFTSFRDS
                     ACQAASPVKTLGRLWVERHGPLIEIGACAPSFLHHQVRNLVGTLKVIGEGSKPVAFAA
                     AALAARSRAAAGPTAPAEGLYFVAADYPGEPGLAGRQSKKTP"
     gene            complement(8934..9848)
                     /gene="fmt"
                     /locus_tag="P5558_14465"
     CDS             complement(8934..9848)
                     /gene="fmt"
                     /locus_tag="P5558_14465"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRY25566.1"
                     /translation="MRVVFMGTSAFAVPALRALATLPHELLAVYTQPPRPAGRGHHPR
                     RTAVHEAAEALGLAVRTPASLKDEAAQADFLALRPDLVIVASYGLLLRRPVLEAARLG
                     CFNIHASLLPRWRGAAPIQRAIELGDAVSGITLFRMEEGLDTGPMLVRRALEIGPRET
                     AGELHDRLAELAAALLPRFIADLAAGRLAEEPQDDSRTCYARKLEKSEARIDFTQPAA
                     VLERRIRAFDPWPGTWCLAGKERLAVLEARVDEGQGEPGTIIGSPLTVACGEGALELV
                     RVRREGRKPVTAAELQRGFPLPPGTRLT"
     gene            complement(9856..10362)
                     /gene="def"
                     /locus_tag="P5558_14470"
     CDS             complement(9856..10362)
                     /gene="def"
                     /locus_tag="P5558_14470"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRY25567.1"
                     /translation="MSSLEILQPPHPTLKTPAALVDEIDGALVRLMADMLETMYAAPG
                     IGLAAPQVGVSRRVVVIDTSEEKNAPVRLLNPTLTWRSDTFDVAEEGCLSLPGQFADV
                     KRHTAVVASYLDPSGKERTLEAEGLLARCLQHEIDHLDGILFTDHLSALKRNMILRRL
                     IKQRRARA"
     gene            complement(10413..11009)
                     /gene="recR"
                     /locus_tag="P5558_14475"
     CDS             complement(10413..11009)
                     /gene="recR"
                     /locus_tag="P5558_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRY25568.1"
                     /translation="MAAADIERLIRLLGRLPGLGPRSARKAGLAMLKRPDALLRPLLD
                     ALATCLERVRVCEVCGNLDTVDPCSVCRDPERDPQLLCVVQEVEDLWAMERLVGFRGH
                     YHVLGGTLSAIEGRSPETIRIAGLLERVAGGRVSEVVLALGATVDGQTTAHYLHERLR
                     PFACRVTRLGHGLPVGGELNYLDEGTLEAAMRARQQVA"
     gene            complement(11029..11352)
                     /locus_tag="P5558_14480"
     CDS             complement(11029..11352)
                     /locus_tag="P5558_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRY25569.1"
                     /translation="MKNIQNMMKQAQQLQTKMADMQAKLAEVEMTGEAGAGMVSVTLN
                     GKGEMRRIKIDKSLVDPEDVETLEDLVVAATNNAKQRVEEHVAGEMSKVAGGLPLPPG
                     MKLPF"
     gene            complement(11352..13214)
                     /locus_tag="P5558_14485"
     CDS             complement(11352..13214)
                     /locus_tag="P5558_14485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF006585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRY25570.1"
                     /translation="MDLFAGSDQPYRVLARTYRPQKLSELIGQDALVRTLTNAMASGR
                     VAHAFLLSGIRGVGKTTTARIIARGLNCTGPDGQGGPTPEPCGECSSCRSLVEERPLD
                     VIEMDAATRTGIDDIREIVDAVRYAPTASRYKIYIIDEIHMLSEKAFNGLLKTLEEPP
                     PHAKFIFATTEVRKLPVTVLSRCQRFDLKRVETPVLEAHLARITEAERIEVAGDALRL
                     VARAAEGSVRDALSLLDQAIVLGQGTVDAATVAGMLGLGDRLKMLDLFERVMGGDAAG
                     ALDSFRELFALGAEPVAVLEDLLEIAHWVSCLEVDRDATSIFAGTPEPVAAGRRLAER
                     LDLAVLARAWQVLLKGLDEVRGAPHAAAAAEMVLMRLAAMGRLPTPGELARRLDGAEI
                     ADDRKPAASSPPVAPRSPLPTPPLARVAPLRPSPPDQRRNPARASPPAGRRPEQSDRG
                     ASEPVDFGDLVRLLNRHGERLLAAALHQGAHLVAFQKDRLELRLDPHLPGDLVGRLSE
                     ALLRLTGRRWLIALSQSAGAASLADQAVANRERLIRELAEHEAVREVLDAFPGARIID
                     IRDRIPADDRTDAPATGDEARYEDNEHEVDHRPLDDDEADEDGASGHDDSRMER"
BASE COUNT         1923 a         4452 c         4866 g         2037 t
ORIGIN      
        1 gggctcgaac cgggccctca tcgagcggct ggtcgcccga ttcgactgcc atgtcggcgg
       61 cggcatccgt gacgtcgaaa cggcgctctt ctggctcgat gcgggggcgc ggcggatcat
      121 cctcggcacc atggcgaagt cggaaattct cgaggcactg ccgaaggatc ggttgatcgc
      181 cgccctcgat gcgcggcacg gcgaggttgt ggtcgagggc tggaaggagg gcaccggccg
      241 gggcatcctg gaacgcgtgg ccgagctcaa ggacttggtc gacggcttcc tcgtcacctt
      301 cgtcgagcgc gaggggcgga tgggcggcac cgacatggcc ctcgcccgga ccatcgtcga
      361 ggcggccgct ccggccaggg tgatcatcgc aggcggcgtc accacgccgg ccgagattgc
      421 cgagctggac gccatgggtg ccgatgcgca ggtcggcatg gcgctctact cgggccggct
      481 cgggctcggc gaatccgttg ccgggatctt caggagcgac cggccggacg gactcatccc
      541 gaccgtggtc accgacgagc gcggtacagc gctcgggctc gtctattcga gccaggagag
      601 cattgcggct gcggtcgacg agcggcgcgg catctaccag tcgaggcggc gcgggctatg
      661 gcgcaagggc gagagcagcg gcgatgtgca ggagctgctg cgcgtcacac ccgactgcga
      721 ccgcgattcg gtgcgcttca ccgtgcgcca agccggggcc gggttctgtc acctcgcgac
      781 ccgcacctgc ttcggtgagg atgccgggct cggccggctg caccgccgcc tgctggcccg
      841 ccggagcgat gcgcccgaag gctcctacac ggcaaggctg atggccgagc cgacgcggct
      901 cgcggccaag gtcacggagg aagcgggcga gctcaacggg gccgccagcc gtgacgaggt
      961 ggtctgggag gccgccgacc tgctctactt cacgctcgtc cgtctcgccg ccgagggcat
     1021 cgggctcgac gaggtggagc gccatctcga ccggcgcgag cggcgtgtcc ggcggcgtga
     1081 ctgaagaccg cctgcatcgg ctgatcgtct ggctccggcc gctgctggag ctgcggctgc
     1141 tgcttgccgt gctgcttctc ggcaccctcg cctggacgtt catcgagctc gccgaggagg
     1201 tggtagaggg cgacacccgg gcgatcgacg aggcgctgct gctgggcctg cgcaccgcgg
     1261 ccgacccggc ggtgccaatc gggccagaat ggctgcaggt ggtgatgcgc gacatcacgg
     1321 cactcggcgg ctacaccata ctctttctcg tcaccgccgc caccgtcatc tatcttttcc
     1381 tcgtcggtag gccgcgcacc gctgtcttca tcctggtcgc cgtcttgggc gccacgcttt
     1441 tgacggtgct cttgaagctc ggcttcgcgc ggccccggcc ggatctcgtg ccccacgcca
     1501 tgcgcaccgc gtctgcgagc ttcccgagcg gccattcgag cggtgccgcc gcggtgtacc
     1561 tcacgctcgg cgccattctc gcaagcttcc aggaccgccg ccgggtcaag ctcttcatca
     1621 tggggctcgc cgtcgtggtc accatgctgg tcgggatctc ccgcctctat ctgggggtgc
     1681 actggccctc cgacatcatt gccggctggg cgctcggcgc cggctgggcc gtgctctgtt
     1741 gggtggtcgc ggcctatctg cgaagggcaa gggtgctgca cagcccgccg gcgggctgat
     1801 ccctcggtcc gcggcgggcg gctcagccgg cgagccagga tgcttcgagc ggggccgcgg
     1861 attccacgac ctcggcgagc ccgagcgccg gcggcagcag gcgctccacg tagaagcggg
     1921 cgaggccggg ccacgcgtga ccgcgcgggt cgtcctcggc tgcatgggca cccatcgcca
     1981 cgagatgggc gccgagcacc cagccgaaga ggtcgaggta gggggtggcg gcggcttcgc
     2041 gcgcttcgcc ggaggcgcct tgcatgtgca gggtggcctg cttcagccgg tccagggcct
     2101 gcgtcagggt cggccgcagc gcctcgtcga gccgggtctc ctcgagctcg gcgaaaagct
     2161 gccaaggcag ctggccgtct tcgatcctga gcttgcggcc ggcgaggtcg agggcctgga
     2221 ttccgttggt gccctcgtag atcggcagca cgcgggcgtc gcggaagagc cgggcgaggc
     2281 cggtctcctc gacatagccg gcgccgcccg ccacctggat ggcgtcggag gtgatggcga
     2341 cggcgccatc ggtgcaccag gccttgacga tgggcgtgag cagcgcgacg cggaggtcgg
     2401 cgcgacgccg ttcctcaaga tcgggatcgc ggaccgcccg gtcgacggcg gcggcggtga
     2461 cgaggcagag cgcgcgcatg gcggcgatgc gggcgcgcat gccgaggagc gtgcggcgga
     2521 tgtcggggtg atcgaccagg gccgccggcc ggccgtggcg ctggccctgc cggcgttcgc
     2581 gggcataggc gagagccccc tggaaggccc gctcggcgag gcccaggccc tgcacgccga
     2641 cgctgatccg cgcattgttc atcatcgtga acatggcggc gagcccgcca tggggggcgc
     2701 caaccagata tcccaccgca cccggcccgt cgccataggc gaccacacag gtgggcgagg
     2761 cgtgcaggcc gagcttctcc tcgagcttca gcacgcgcca gtcgttgcgg ctgcaatcgg
     2821 gcagtcgctt gggcacgagg aagagcgaga tgcccttgct gcccgaaggc gcgtccggca
     2881 gccgggcgag gacgaggtgg acgaggtccg aggtcaggtc atggtcgccc cagctgatga
     2941 agaccttctg gcccctgagg cggtgatgcg gcccgagcac cgggtcgtcg gccggttcgg
     3001 ccgtgctgcg gatggcggcg aggtcggagc cggcctgcgg ctcggtcagg cacatggccc
     3061 cggtccaacg cccggcgacg agcggcggca gccagctcgc ctgctggtcc cggtcggcat
     3121 ggcgcagcag cgcctcgatg gtgccctggg tgagcagcgg gcagagctgg aaggtgatgt
     3181 cggcggcggc gaaaatctcg gccacggccg tgttcagcgt ccagggcagg ccctggccgc
     3241 catgctcctc cgggaagacg aggcccatcc agccgccctc ggcgaagcgc tcgtaggctt
     3301 gccgaaaacc cggagcagta tgcacctcac cgtcccggag catggcaccc gggcgatcgc
     3361 ccgtggcatt cagcgggacg agctcggctt cggcgaaccg gccggcctcg gccaggacgg
     3421 cagcgaggtc gtcctgatcg atttcggcgt ccttgggcgg cccggcgatc cgctcgatga
     3481 cgaagccgat ctcgccgagc ggtgcccggt aggtggtcat gcgggcgggc tgggggtgat
     3541 gacggcgagc acggcctgct cgagcgcccc cgaggcgacg cagttctggt cgaagaggtt
     3601 gttgtcgaac cggtcgcggt agacaccgta atagttcgtc ttcatgtcga cattcgtctg
     3661 cgtgccgagt gaggtgacgc ggatctgcac aagtgtcttg tgatcctcgg gatcgaccag
     3721 ccgcgagcgc gacgagtcgc cgctcgaatc ctccacccgg atcttgtcgc agacgaacca
     3781 ttcggccggg gcaccggtca ggatgccggc acggatcatg cccggtgcgc tgtcgggctc
     3841 gagcataaag ccgagctcgc gcaggcgcgt ctcgagggct gcctgcgtag acggcacatc
     3901 ctgcgtcgtg acttgggcac gggcggggtc gggcctggcg gggccgggtc ccgaagaggc
     3961 gcaggcggcc aggagaagga ggccggcgac cattggcaat atgtgacgca tcaggaaatc
     4021 tctctagagg caggcgcatc gggtgcggcc aggatgacac cgggattgag aatgccggtc
     4081 gggtcgaggg tcgccttcag ccgccgcatg agctcgagct cgagcgggtc cttgcgacgc
     4141 tcgagctcgc cccgcttcag gcgaccgata ccatgctcgg cgctgaacga gccgccgaac
     4201 tcggcgacca gcatcatcag gtgctcgttc agcagggacc ggagctcggg cacgccctcg
     4261 cgatcccgag ggatcaggaa attgaagtgg agattgccgt cgccgacatg gccgaaggga
     4321 aagagctgga cgtcgggggc cagcttcccg aaatgcgctt ccgcccgggc gatcagcgct
     4381 ggaaggtcgg cgatggcgac cgagatgtcg ctgcgctgca catggccgac cttggtcgtc
     4441 gcctcgctct gaccttcgcg gatgagccac atggcggtcc gctgcgcctc gttctgggcg
     4501 atcgtgccgt cctggacgag gccggcgtcg aagcccgctt ccagcgcccg gtcgaccggt
     4561 acgtcgaggc caccatcgag agaccaggcg acctcgatca ggagatgcca ggggtgcggc
     4621 tcggcgagcg gccggcgacc acccggcaga tggtcgttcg ccgcctccac gccgagcccg
     4681 gcgatcagct cgaagctgga gatcagctcg cccagatgat cctgaaagag gctgaggaga
     4741 cggagcgcat cggccgggct ctcgatgccg agccagatgg tgcggcgctc gcgcggccga
     4801 gggtagaggc gcagcacggc ggcggtgatg atgccgaggc tgccctccgc accgatgaag
     4861 agctgcttca ggtcgtagcc ggtattgtcc ttccggagcg cccgcaggcc gttccacacc
     4921 cggccgtcgg ccaggaccac ctcgaggccg agcacgagct ggcgggtcat gccgtagcgc
     4981 agcacgttca cgccaccggc attggtcgag agattgccgc cgatccggca gctgccctcc
     5041 gcaccgagcg aaagcgggaa gagccgcccg gcgccgtcgg ccgcggcctg gacgtcggcc
     5101 aggatgcagc cggcctcggc gacgagcgag ccaccggcgg cgtcgacggc gcggatccgg
     5161 ttgagccgct cgaggctcag gaccacctgg cggccggagc catccggaat gccgccggcg
     5221 acgaggcccg aattgccgga ctgtggcacg atggcgacgc cgagccgggc cgcctcccgg
     5281 acgatggcag ccacggcctc ggtcgaatcg ggacgcagga cgagcggcgt ggtgcccttg
     5341 tgctggtgcc agaaatcgac cgtgtagcgc tcgatcccgg ccgcctcggt aatgatcccc
     5401 tggggaccca gcaggggtgc cagggcggct accagattct cgatgggcaa tgcgctcagc
     5461 gtcatgggtc gtcgtcatcc cggcgaaaca tcgccggaac gtagaaacct tggaggctgg
     5521 cggcaagctg gctcggttga cagccctagg ccccaagcta ggttacgccg gctcaatagt
     5581 gacagggaca gcgaccgatg gacgtggaac gcctcaaggc ggtgatcgag gcgggtttcg
     5641 aggaccgtgc aacggtcgac gcttcgacca agggtgagct caggacagcc gtcgaggcgg
     5701 cgttggccgg gctcgatgcc ggcaagttcc gggtcgccga gaagggccgc aagggctggg
     5761 tcgtccacga gtggctgaag aaggcggtgc tgctgtcctt ccggctcaac gccatggagc
     5821 gcatcgacgg tgcgcccggc ggtgcttcct ggtgggacaa ggtgccatcc aagtttgccg
     5881 gctggcgcaa gaaggatttc accgaagccg gcttccgcgc cgtgccgggc tgcgtcgtcc
     5941 gccactccgc ctatatcgcg ccgaacgtcg tgctgatgcc atccttcgtc aatctcggcg
     6001 cctatgttgc cgaggggacg atgatcgaca cctgggccac agtcggctcc tgcgcccaga
     6061 tcggccgcaa ctgtcacatc tcgggcggtg ccgggatcgg cggcgtgctc gagcccttgc
     6121 aggcgaaccc ggtgatcatc gaggatgact gcttcgtcgg tgcccgcgcc gaggtcgcgg
     6181 aaggtgtcgt ggttgagacc ggctcggtgc tctcgatggg cgtctatctt ggtgcctcga
     6241 ccaagatcat cgaccgggcc acgggcgaga tcttcgccgg ccgggtgccg gcctattcgg
     6301 tggtcgtgcc gggcacactg cccggtaagc cgctgccgga cggcagcccc ggcccggggc
     6361 tctattgtgc ggtgatcgtc aagcgggtcg atgccaggac caggagccgg acgtcgatca
     6421 acgagctgct gcgcgactga cggcggcctg gcgatgtcgt ctgtcgatcc ggtcgagctg
     6481 acccgggcgc tggtccgctg cccgagcgtg acgccggcgg atgccggggc gcaggcggtg
     6541 ctggccgagg tgctggaagg gctcggcttt cgcgtcgagt tgttgcgcct gggtgacggc
     6601 ggcgagccgg ccatcgacaa cctcttcgcc cggctcggcg atggctcgcc gcatctcgcc
     6661 ttcaacggcc acaccgacgt ggtgccggct ggcgacgatg ccgcctggtc ggccgggccg
     6721 ttcgccgccg agatcaggga cggctggctc tacggccgcg gtgccgccga catgaagggc
     6781 gacatcgcct gcttcgtcgc cgccatcgcc cgctatctcg aacggcacgg gtccgtgccg
     6841 ggctcgctca gcctgctgat caccggcgac gaggagggac cggcggtcaa cggcacggtg
     6901 gcgctcctgc gctgggccga ggcggccggg caccggttcg acgcctgtct ggtcggcgag
     6961 ccgaccagcg tcgacgaact cggcgacatg gccaagatcg gccggcgcgg ctcgctcacc
     7021 gtgacgctcg aggcccgggg caggcagggc catgtcggct acccgcatcg cgccgacaat
     7081 gccgcgcacc gcctcgtgcg ggtgctggcc cggctggtcg agaccccgct ggacgagggc
     7141 accgagctct tcgagccatc gagcctgcag gtgaccacca tcgacatcgg caatccggcg
     7201 agcaacgtcg tgccggcccg ggcgcgggcc gtgctcaaca tccgctacaa cgacagccag
     7261 agcccggcca gcttgaaggc gctcgtcgct gcggccatcg aggcggcggg cgggcgggtc
     7321 gaggccgcct gcaccgaggg ggccaatgtc ttcgcctgcc cgcctgggcc gctcgccgcc
     7381 ctgctcggtg acagtgtcga ggcggtgacc ggccgccgtc cggtgctctc gacctccggt
     7441 ggcacctccg atgcgcgctt catccacagc cactgcccgg tgatcgagtt cggcctgatc
     7501 ggtcagacca tccaccaggt cgacgagcgg gtgccgctcg tcgagctcca agcgctgaca
     7561 gccatttatc tcgagttcct cgaacgctac ttcgggcaat gatgccgccc ttccccgaag
     7621 ttcgtcatta tgccgagggg gcgtggcggc tggcccgcgg cgatgccggc ggcatggggc
     7681 atttcgactg cacggtcgac ggctactggc gctccttctg ggcggccttt tacgttctgc
     7741 cggcctatgc gctactggtc gccgaccagc acgcccgggc ccaccgggac aatggctttc
     7801 tttccatcgt ggtcggcgag gggttggtct acgtcctcgg ctggctggtg atgccggtgc
     7861 tcgccatctt cctcacccgc ttcttcgggc tgcagcggca ttacgtgccg ctgatcgtgg
     7921 cgctcaactg ggcatccctg gtgcagatcg ggatcctcct cgtgccgctg gccctgggcg
     7981 tcctcctcgc accgtcgctc gccgccctcc tcatgctgct cgcgagtggc gctgtgctcg
     8041 tctacaagac gttcgtcgcc agggtggcgc tcgataccac gatcggcacg gcggcgagct
     8101 tcatcgcggc cgagctcgtc atcatgctga tggtgaccgg ctggctctac ggcgtcttct
     8161 tcgactgacg gccggcgagg ccgggctcgc ccgggtagtc cgcggcgacg aaataaagcc
     8221 cctcggccgg ggcggtcgga ccggccgccg ctcggctgcg ggcagccagg gcggcggcgg
     8281 cgaacgcgac cggcttgctg ccctcgccga tcaccttcag cgtgccgacc agattgcgca
     8341 cctggtggtg gaggaaggag ggggcgcagg cgccgatctc gatcaggggg ccgtgccgct
     8401 cgacccagag ccggcccagt gtcttgacgg gcgaggcggc ctggcaggcg ctgtcgcgaa
     8461 agctcgtgaa gtcgtggcgc ccgaccagcg tctcagccgc cgcctgcatc cgctcggcgt
     8521 cgagcgagcg agcgacgtgc cagacccggc cgtgttccag cgcaggcggc gctcgccgcg
     8581 cgaggatccg gtagcgataa tgccgggcgg tagcgttgaa ccggacgtga aatccatcct
     8641 gggccagact ggcccacagc accacgatcg gcagatggcg gagatggtgg ttgagcccgc
     8701 tcatcagccg ctcgggcatg atcgtctgct cgaggtcgag ttggaccggc agggccaggg
     8761 catgcacgcc ggcatcggtc cgcccggcgg cgacggccgg acccatgcgc cgcaccatgc
     8821 ggtcagcagc cgcctccagc gccgcctgca cgctcggtcc gttggcctgg atctgccagc
     8881 cgcagaaggg gccgccatcg aactcgagca gcagccgata gcggggcatc tgcctaggtc
     8941 agccgagtgc cggggggcag cgggaagccg cgctgcaact cggcggcggt cacgggcttg
     9001 cggccttcgc ggcggacgcg gaccagctcc agcgcacctt cgccgcaggc gacggtcaac
     9061 ggtgacccga tgatcgtacc gggctcgcct tgcccttcgt ctaccctggc ctcgaggacg
     9121 gcgaggcgct ccttgccggc gaggcaccat gtgccgggcc agggatcgaa ggcgcgaatg
     9181 cgccgctcca gcaccgcagc cggctgcgtg aagtcgatgc gcgcctcgga cttttccagc
     9241 ttgcgggcgt agcaggtgcg ggagtcgtcc tgcggctcct cggcgagccg accggccgcg
     9301 agatcggcga tgaagcgcgg cagcaaggcg gcagcgagtt cggccaggcg atcgtgcagc
     9361 tcgcctgcgg tttcgcgagg gccgatctcc agcgcccggc gcaccagcat cggcccggtg
     9421 tcgagccctt cttccatgcg aaagagagtg atgccggaga cagcgtcgcc gagctcgatc
     9481 gcccgctgga tcggtgcggc gccgcgccag cgcggcagga gcgaggcatg gatattgaag
     9541 cagccgagcc gggcagcttc gagtaccggc cgccgcagca ggaggccgta ggaggcgacg
     9601 atgacgagat cggggcggag ggcaaggaag tccgcctgcg ctgcctcgtc cttgagggaa
     9661 gccggggtgc gcacggcgag gccgagcgcc tcggccgcct cgtgcaccgc cgttcgccgc
     9721 gggtgatggc cgcgtccggc cggccggggc ggctgggtgt agacggcgag caactcgtgc
     9781 ggcagggtgg cgagggcacg cagcgccggc acggcgaaag cgctggtgcc catgaagacg
     9841 acacgcaacg ggcgctcagg cgcgggcacg gcgctgcttg atgaggcggc gcaggatcat
     9901 gttgcgcttg agcgctgaga gatggtcggt gaacaggatg ccgtccagat ggtcgatctc
     9961 gtgctgcagg cagcgggcga gcaggccctc ggcctcgagc gtgcgctcct tgccggaggg
    10021 gtcgagatag ctcgccacca cggccgtgtg gcgcttcacg tcggcgaact ggccgggcag
    10081 gctgaggcag ccctcctcgg cgacgtcgaa ggtgtcggag cgccaggtga gggtcgggtt
    10141 gagcaggcgg accggggcgt tcttctcctc gctcgtatcg atcaccacga cccggcgcga
    10201 gaccccgact tgcggggcgg cgaggccgat gcccggggcc gcgtacatgg tctccagcat
    10261 gtcggccatg aggcgtacca gggcgccatc gatctcgtcg accagtgcgg ccggcgtctt
    10321 gagggtgggg tggggtggtt ggaggatctc gaggctggac atgatctcgc gtgaattctg
    10381 atgaggcagg aatatcggga agcgaaggcg gctcaggcga cctgctggcg ggcacgcatc
    10441 gcggcttcga gcgtcccctc gtccagatag ttgagctcac cgccgaccgg aagaccatgg
    10501 ccgagccgcg tcacgcggca ggcgaagggg cgaagccgct cgtgcaggta atgggcggtg
    10561 gtctgcccgt cgacggtggc gccaagggcg agcacgacct cgctgacccg gccgccggcc
    10621 acccgctcga gcaggccggc gatgcggatg gtctccgggc tgcggccctc gatcgccgag
    10681 agcgtgccgc cgagcacgtg gtagtggccg cgaaagccga ccagccgctc catggcccag
    10741 agatcctcga cctcctggac gacacagaga agctgcgggt cgcgctccgg atcgcggcag
    10801 acgctgcagg gatcgacggt gtcgagattg ccgcatacct cgcagacccg cacccgttcc
    10861 aggcaggtgg caagtgcatc gagcagcggc ctgaggaggg catccggccg cttcagcatg
    10921 gcgagcccgg ccttcctggc ggagcgcggg cccagacccg gcagccgccc gaggagccgg
    10981 atcaaccgct cgatgtcggc ggcggccatg gctgtcgatc cgccccgttc agaacggcag
    11041 cttcatcccg ggcggcagcg gcaggccgcc ggccaccttg ctcatctcgc ccgcgacatg
    11101 ctcctcgacc cgctgcttgg cgttgttggt ggcggcgacc accaggtcct cgagcgtctc
    11161 gacatcctcg ggatcgacca gggacttgtc gatcttgatt cggcgcatct cgcccttgcc
    11221 gttgagagtg acgctgacca tgccggcgcc ggcctcgccg gtcatctcga cctcggcgag
    11281 cttggcctgc atgtcggcca tcttggtctg cagctgctgc gcctgcttca tcatgttctg
    11341 gatgttcttc atcaacgctc cattcggctg tcgtcatggc ccgaggcgcc gtcctcgtcc
    11401 gcttcgtcat cgtcgagcgg ccgatggtcg acctcatgct cgttgtcttc atacctggcc
    11461 tcgtcaccgg tcgccggggc atctgtccga tcgtctgccg ggatgcgatc tcgtatatcg
    11521 atgatccgcg ctcccgggaa cgcgtccagc acctcgcgca ccgcctcgtg ctcggcaagc
    11581 tcccggatca gccgctcgcg gtttgccacc gcctggtcgg cgaggctggc ggcaccggcg
    11641 ctctgcgaga gggcgatcag ccagcgccgg ccggtgagcc gcagcagcgc ctcgctcagg
    11701 cggccgacga gatcgcccgg cagatgggga tcgaggcgca gctcgaggcg gtccttctgg
    11761 aaggcgacga gatgcgcacc ctggtgcagg gcggcggcga gcagccgctc gccgtgccgg
    11821 ttgagcagcc gcaccaggtc gccgaagtcc acgggctcgc tggcgccccg atcagactgc
    11881 tccggccggc ggccggcagg cggcgaggcg cgtgcgggat tccgccgctg gtcgggcggc
    11941 gagggccgca ggggggcgac ccgtgccaaa ggcggcgtcg gcaaaggcga cctcggcgcg
    12001 acggggggcg agctggccgc cggctttcga tcgtccgcga tttcggcacc atcgagccgg
    12061 cgtgcaagct cgcctggggt cggcagcctg cccatggcgg cgaggcgcat gaggaccatc
    12121 tcggcggccg ccgcggcatg tggcgcgccc cgcacctcgt cgagtccctt gagcagcacc
    12181 tgccaggcgc gggccagcac ggcgagatcg agccgctcgg cgaggcgccg gccggcggcc
    12241 accggctccg gcgtgccggc gaagatcgag gtggcgtcgc gatcgacctc gaggcagctg
    12301 acccaatgcg cgatctcgag caggtcctcg agcactgcca cgggctcggc gccgagcgcg
    12361 aagagctcgc gaaagctgtc cagggcaccc gcggcgtcgc cgcccatgac acgctcgaag
    12421 agatcgagca tcttcagccg gtcgccgagc ccgagcattc cggccaccgt tgccgcgtcg
    12481 acggtgccct ggccgagcac gatcgcctgg tcgagcagcg agagggcgtc gcgcaccgag
    12541 ccttcggcgg cccgggcgac gagcctaagt gcgtcgcctg ccacctcgat ccgttccgcc
    12601 tcggtgatcc gcgccagatg cgcctcgagc accggcgtct ccacccgctt gaggtcgaag
    12661 cgctggcagc gcgacagcac ggtgaccggc agcttcctga cctcggtcgt ggcgaagatg
    12721 aacttggcgt gcggcggcgg ctcctcgagc gtcttgagaa ggccgttgaa ggccttctcg
    12781 gagagcatat ggatctcgtc gatgatgtag atcttgtagc gcgacgcggt gggtgcgtag
    12841 cggaccgcgt cgacgatctc gcggatgtcg tcgatgccgg tgcgggtcgc cgcgtccatc
    12901 tcgatcacat cgagcggtcg ctcctcgacg agggagcggc aagagctgca ttcgccgcag
    12961 ggctcgggtg tcgggccgcc ctggccatcc ggcccggtgc agttgaggcc gcgcgcgatg
    13021 atcctggccg tcgttgtctt gccgacgccg cgaatgccgg agagcaggaa ggcgtgggcg
    13081 acccggcccg aggccatggc gttcgtcagc gtgcgcacca gcgcgtcctg gccgatcagc
    13141 tccgacagct tctgcggccg ataggtgcgg gcgaggaccc tgtacggttg gtcggagccg
    13201 gcgaagagat ccatcgagaa ggcgctttcg ggccggtgcg cgggcaccgg tggacgaagg
    13261 gggctcatgg cggactaa
//
LOCUS       DAOTFI010000138        13187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5629_length_13187_cov_8.829272, whole genome shotgun sequence.
ACCESSION   DAOTFI010000138 DAOTFI010000000
VERSION     DAOTFI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13187
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5629_length_13187_cov_8.829272"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2126
                     /locus_tag="P5558_14490"
     CDS             <1..2126
                     /locus_tag="P5558_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HRY25571.1"
                     /translation="FHMDMTYRGIINMLVMCGCVGQSGGGWSHYVGQEKLRPQTGWTA
                     LAFALDWNRPPRHMNSTSAWYAHTDQWRYETVSPKEILSPTAPEGNWDGSLIDFNIRA
                     ERMGWLPSAPQLKTNPLDLGRAAETAGQDVRDYVAERLKSGELEMSCEDPDAPENWPR
                     NLFVWRSNLMGSSGKGHEYFLKHLVGTLHGVLGKDLGTSEGSLRPLEAKWHDEAPEGK
                     LDLLVTLDFRMSTTAVYSDIVLPTASWYEKDDLNTSDMHPFIHPLQAAVDPAYESRSD
                     WEIFKTIARQFSRIAPEVLGVERDVVAVPLLHDTPGELAQDQVRDWKRGECDLIPGRT
                     APNFVAVERDYPNLYRRFTSLGPLMAKIGNGGKGISWDTRTEVHHLVALNEPVQEDGP
                     TKGLARIETAIDAAEVVLMLAPETNGEVAVKAWDALGRITGRDHTHLAEVKEDEKIRF
                     RDIAAQPRKIISSPTWSGIESEEVCYNAGYTNVHELIPWRTLSGRQQLYQDHLWMRAF
                     GEALAVYRPPVDLKTVTSAINMDARDGKPHVVLNFITPHQKWGIHSTYTDNLLMLTMS
                     RGGPIVWISEVDARTAGVVDNDWIELYNANGALCARAVVSQRIKPGTTLMYHAQEKIV
                     NVPGSQMTGRRGGIHNSVTRTVLKPTHMIGGYAQLSYGFNYYGTVGSNRDEFVILRKM
                     TKVDWLDRPAASAAAAEGSVAKMEV"
     gene            2128..3657
                     /gene="narH"
                     /locus_tag="P5558_14495"
     CDS             2128..3657
                     /gene="narH"
                     /locus_tag="P5558_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HRY25572.1"
                     /translation="MKIRAQIGKVLNLDKCIGCHTCSITCKQVWTSRDGVEYAWFNNV
                     ETKPGIGYPKDWENQKRWRGGWERNSRGHLQPRQGAKWRILANIFANPDLPEIDDYYE
                     PFDFDYGHLQSAPEMEHFPTARPRSKITGERIEKIEWGPNWEEILGGEFEKRSKDYNF
                     EGIQKEIYGQFENTFMMYLPRLCEHCLNPSCAASCPSGAIYKREEDGIVLIDQEKCRG
                     WRMCVSGCPYKKIYYNWDTGKSEKCTFCYPRIESGQPTVCSETCVGRIRYLGVMLYDA
                     DAIEKAANAENETDLYQAQLDIFLDPNDPVVQEMALADGVPQDWLEAAKRSPVWKMAM
                     EWKVAFPLHPEYRTLPMVWYVPPLSPIKNAAEAGLIGHDREMPDVRSLRIPLRYLANL
                     LTAGDEAPIATCLERMLAMRGYMRAKTVDGVIDEGIAERVGLTPAQIEDMYRIMALAD
                     YEDRFVIPTAHRELAEEDIMGLRGGCGFTDGNGCSDGLSKGSLFGNRRRGLTIPEAMK
                     S"
     gene            3654..4361
                     /gene="narJ"
                     /locus_tag="P5558_14500"
     CDS             3654..4361
                     /gene="narJ"
                     /locus_tag="P5558_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRY25573.1"
                     /translation="MTLTLKALSALLTYPDAALARGTAEIETALRDEALLDASAIEGL
                     RPLLAELGSRDLFDLQERFVLLFDRSRSLSLNLFEHVHGESRARGQAMVDLLETYRAG
                     GFEPVGPELPDHLPMLLEFLAARPLDEAREVLADTVHILVTLRDRLDRRQSPYAPVFA
                     ALVSLAAAPPAAEALAEIAAHPEDDPEDLAALDAVWEEAAVTFGPDPNAGCPAGREML
                     SRMGPPAQPPAWAGGGS"
     gene            4378..5091
                     /gene="narI"
                     /locus_tag="P5558_14505"
     CDS             4378..5091
                     /gene="narI"
                     /locus_tag="P5558_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HRY25574.1"
                     /translation="MNFFLFQVYPYIALTVLALGSIARYETDPFTWKSSSSQLLRRRQ
                     LLLGSILFHVGVLIVFLGHFVGLLTPIGIFDALGIGHTFKQLLAMIVGGVAGVFALAG
                     GVILLHRRLYDPRIRATSSFADIGILALLDLQLVLGLLTIVVSAQHLDGGEMVRFMGW
                     TQAIFTFRTDAWLLVAEASWIFKLHIFLGLTIFVLFPFTRLVHMLSVPVRYLWRPGYQ
                     IVRSRKAGPAPAARPHPAE"
     gene            5096..5893
                     /locus_tag="P5558_14510"
     CDS             5096..5893
                     /locus_tag="P5558_14510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY25575.1"
                     /translation="MRGPLQPDVVVNAERIPARLIAAEAQNHAAPPGKPGHAWRAGAR
                     ALAVRALLLQEARRLGLAPEPRDLGAGRREVPEEALIRAVIERRMQPVPPDEDACRAF
                     YDRHAAHFRAPAVYEASHILIAAPEDADARTRADAAARTLIGVLATRPQDFADMARRH
                     SDCPSRANGGRLGQLCAGDTAPEFEAALGGLAVGEITPEPVATRYGSHIIRLDAREQG
                     AILPYDLARPRIRETLERLAWSHAAKSLVAALLEDATITGVDFDHAA"
     gene            complement(6198..7271)
                     /gene="ugpC"
                     /locus_tag="P5558_14515"
     CDS             complement(6198..7271)
                     /gene="ugpC"
                     /locus_tag="P5558_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY25576.1"
                     /translation="MATIRLKDVRKNYGEVQVIKGVNIDIDEGEFVVFVGPSGCGKST
                     LLRMIAGLEDISSGTLEIGGRVVNDLPPKDRGIAMVFQSYAIFPHMTVRENVAFGLTL
                     SRTPKAEKERKVAEAARILQMEHLLDRRPSQLSGGQRQRVAIGRAIVRDPSVFLFDEP
                     LSNLDAALRMDMRMEIGKLHQSLNASMIYVTHDQVEAMTLADKIVVLRGGEVMQIGSP
                     MELYHNPANLFVAGFLGAPSMNFMTVDVRDMGAEGATIANGSLDPISVGTKGRPIVKG
                     GKATLGIRPQYMQPTAPDQGMLHGTVALTERLGSETVVDVTLRDGSQVIAAIAEDRVL
                     QPGSEIGLSFDPAMAHLFDEALT"
     gene            complement(7284..8276)
                     /locus_tag="P5558_14520"
     CDS             complement(7284..8276)
                     /locus_tag="P5558_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050053 - levansucrase activity [Evidence
                     IEA];
                     GO_process: GO:0009758 - carbohydrate utilization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 68 protein"
                     /protein_id="HRY25577.1"
                     /translation="MVLALDTHWIWDSWYAHDGEHWHGYFLKAPKSLGDPQLRHRNAR
                     HGHAVSDDLVSWTHLGTCLEPSEGPGWDDCTTWTGSVLQGPDGTWHLFYTGASRAEDG
                     LYQRIGHAVSTDMHNWKRVGEAPCLDLVGPHAASYEASFQDGFWHDRAMRDPWVMADP
                     NGDGWLMFFTARAAGVNEPNAGGAIGFATSPDLFDWVLEPPVFVGGYGQLEVPQVFEQ
                     GGRWYCLFCTAAEHFSKDQAETHPGGPVSGSHYLIGEGPRGPWRIAPGHFLDGALPCR
                     RYAARILKTDAGLKIIGFADYPAGQFVGQVTDPDPVEIDADGMLRVQGAQLAAE"
     gene            complement(8288..9166)
                     /locus_tag="P5558_14525"
     CDS             complement(8288..9166)
                     /locus_tag="P5558_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25578.1"
                     /translation="MATISQDNHGLRLAVRYLVLVLVAVIFIFPLVFMVVSSLKPDQQ
                     LLSDTGSLRAFLPVGDIGLSNYTHAFQRAPVGLFVFNSVFVTGLTVLICLLVCSLAAF
                     AFVFLEWRGRAVVLSIILATLIVPFETIAIPLLLLVSKLPWIGFSGIEHGWLNTYRVQ
                     ILPWAADGLTIFLFVQYFKDLPRELIEASRVEGATWLQIYLKVVMPLAGPVLATAAIL
                     KFLVMYNQYLWPLIVVQDEAYRPVMVGLQYFFQLNTAWGEIMAYLSLITIPVLAFYLV
                     LQRAFIASIASTGVKG"
     gene            complement(9166..10236)
                     /locus_tag="P5558_14530"
     CDS             complement(9166..10236)
                     /locus_tag="P5558_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25579.1"
                     /translation="MASSLLRSDRAGWGFAIPAFALIGTFIVLPFFFAFYLSLTNQRL
                     ISPNPTEFVGLDNYRSLLGVAVVTLEPERGDDGAVLRDEDGNIEYPSLRSVTRNDDTP
                     QYRGMREWFRWSSGDDARVVIARDVVFMKALVNTFTFVLVVAPCQAALALGLALLINQ
                     RLRGINVFRGIYFMPVVVSIVVVSLLWRFIYDGQDGLLNNLLSGLTFGLFQPVDWLGN
                     PSTALGSIIVMSIWQAVGFHMVIWLAGLQTISPTLYEAADIEGATKWQRFRFVTWPGL
                     RNTAVLILIVITMQCFALFAQIDVMTKGGPLDSTQTLVFQAVERGYGKQDISGGSTIS
                     VLLFLIVLCISLIQRWLTRERT"
     gene            complement(10292..11557)
                     /locus_tag="P5558_14535"
     CDS             complement(10292..11557)
                     /locus_tag="P5558_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25580.1"
                     /translation="MNIRLTAFATTALGAIWGAQAFAQTELTMWYHGAGNPEESAVLD
                     QIVEEFNASQSDWSVVVQSFPAGAYNDTVIAGALAGNLPDIIDVDGPVMPSWAWAGYI
                     QPLEIDQGTIENFLPTAIGRWKDDIYSVGLWEAAVAMVTRRSVLEKYDIRIPTLAEPW
                     TRDELDAALEAIQEGGEFDYPLDLGMAWSADEWYPYAFAPFLQSFGGDLIDRSTYLES
                     DGVLNGDAGIEFGEWWQSLFDRELVPGTSQDPADRDSGLIDGKYAMSWNGNWAAANAV
                     EAFGDDVLFLPAPDFGNGPKIGAASWQFAVSASSDHPEGANQFIEFMLQDRWQVAFSN
                     AQAVIPATSSAAAESELYAEGAPMAVFYDLSKEQALLRPVTPGYPVMAKIFRKALADI
                     ASGADVADTLDAAADEIDADIEANSGYGN"
     gene            complement(11554..12648)
                     /locus_tag="P5558_14540"
     CDS             complement(11554..12648)
                     /locus_tag="P5558_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY25581.1"
                     /translation="MSTIYDVAKRAGVSPKTVSRFLNGDAPVGRSTRDKVERAIAELG
                     YVPSSAARTMRSNRSGLVGLITGAITLTPRRMALGGLPDIEIVQGILRELEESGLTLL
                     ISDTGGKSERIPNLVRTFQEHRVEGIIFVADHHMKVTLPGIAGDTKLVLANCFDDAGS
                     PAVLPNDRSGQKALTEELIARGHRRIAYLTLGPEMEATRLRVAGYRDALAIAGIPFDP
                     ALVTAANLSADDHLAEEQLLWDALDRVLALEEPPTVLCLGNDRMALRAYGMLRSRGLT
                     LPDDISVAGYDDHTLITETLYPKLTTVKLPYAAMGVRAAQLLTSMVRDGNAAPEAPVL
                     VGGPAVWRDSVRPGPANRISELPMRGRNPA"
     gene            12803..13045
                     /locus_tag="P5558_14545"
     CDS             12803..13045
                     /locus_tag="P5558_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25582.1"
                     /translation="MQKIGRPKGAPPTGPGSPAPTPIGQRQPAAEPVPAGAAYEILHP
                     KLDRGLMRSQCQRWDDFPTHILNKVLYNNILHSVSS"
BASE COUNT         2225 a         4442 c         4440 g         2080 t
ORIGIN      
        1 ggttccacat ggacatgacc tatcgcggca tcatcaacat gctggtgatg tgcggctgcg
       61 tcggccagtc cggcggcggc tggagccact acgtcggcca ggagaagctg cgcccgcaga
      121 ccggctggac ggcgctcgcc ttcgcgctcg actggaaccg cccgccccgc cacatgaact
      181 cgacctcggc ctggtatgcg cataccgacc agtggcgcta cgagacggtc agtccgaagg
      241 agatcctgag cccgaccgcg cccgagggca actgggacgg cagcctgatc gatttcaaca
      301 tccgcgccga gcgcatgggc tggctgccct cggcgccgca gctgaagacc aacccgctcg
      361 acctgggccg cgccgccgag acggccggcc aggacgtcag ggactacgtg gccgagcggc
      421 tgaaatcggg cgagctcgag atgtcctgcg aggatccgga tgcgccggag aactggccgc
      481 gcaatctctt cgtctggcgc tcgaacctca tggggtcgag cggcaagggg cacgagtatt
      541 tcctcaagca cctggtcgga acgctgcacg gggtgctcgg caaggatctc ggaaccagcg
      601 agggcagtct tcggccgctg gaggcgaagt ggcacgacga ggcgcccgag ggaaagctcg
      661 acctcctggt gacgctcgac ttccgcatgt cgaccacggc ggtctattcc gacatcgtcc
      721 tgccgacggc ctcctggtac gagaaggacg atctcaatac ttcggacatg catcccttca
      781 tccacccgct gcaggcggcg gtggatccgg cctatgaatc gcgctcggac tgggagatct
      841 tcaaaaccat cgcgcgccag ttcagccgga tcgcgcccga ggttctcggc gtggagcgcg
      901 acgtcgtggc ggtcccgctc ctgcacgaca cgcccggcga gctggcgcag gaccaggtgc
      961 gcgactggaa gcgcggcgaa tgcgacctga tccccggcag gaccgccccg aacttcgtgg
     1021 ccgtcgagcg ggattatccc aatctctaca ggcgcttcac ctcgctcggc ccgctgatgg
     1081 cgaagatcgg caacggcggc aaggggatct cctgggatac caggacggaa gtgcatcacc
     1141 tggtcgccct gaacgagccc gtgcaggagg acggccccac caagggcctc gcccggatcg
     1201 agaccgcgat cgacgccgcg gaagtggtgc tgatgctcgc gccggagacc aacggcgagg
     1261 tggcggtcaa ggcctgggac gcgctcggca ggatcaccgg ccgggaccac acccatctcg
     1321 ccgaggtcaa ggaagacgag aagatccgct tccgcgacat cgccgcccag ccgcgcaaga
     1381 tcatctcatc gccgacctgg tcgggcatcg agagcgagga ggtctgctac aatgccggct
     1441 ataccaacgt ccacgagctg atcccctggc gcacgctcag cggccggcag cagctctatc
     1501 aggaccatct ctggatgcgg gccttcggcg aggcgctggc ggtctatcgc ccgccggtcg
     1561 atctgaagac cgttaccagc gcgatcaaca tggatgcccg cgacggcaag ccccatgtgg
     1621 tgctgaactt catcacgccg caccagaaat ggggcatcca ctcgacctat accgacaacc
     1681 tcctgatgct gaccatgagc cgcggcggcc ccatcgtctg gatctccgag gtcgacgcca
     1741 ggaccgccgg ggtggtcgat aacgactgga tcgagctcta caacgccaat ggcgcgctct
     1801 gcgccagggc ggtggtgtcc cagcggatca agccgggcac cacgctcatg taccacgcgc
     1861 aggagaagat cgtgaacgtg cccggctcgc agatgaccgg gcggcgcggg ggcatccaca
     1921 attcggtgac gcgcaccgtg ctcaagccca cgcacatgat cggcggctat gcgcagctga
     1981 gctacggctt caactactac ggcaccgtgg gctcgaaccg cgacgagttc gtgatcctgc
     2041 gcaagatgac caaggtcgac tggctcgacc ggcccgcggc gagcgccgcg gccgccgaag
     2101 gctcggtcgc gaagatggag gtctgacatg aagatccgcg cgcagatcgg caaggtcctg
     2161 aacctcgaca aatgcatcgg ctgtcacacc tgctcgatca cctgcaagca ggtctggacc
     2221 agccgcgacg gcgtcgaata cgcttggttc aacaatgtcg agaccaagcc cggcatcggc
     2281 tatcccaagg actgggagaa ccagaagcgc tggcgcggcg gctgggagcg caattcgcgc
     2341 gggcatctgc agccgcggca gggcgcgaaa tggcgcatcc tcgccaatat tttcgcaaac
     2401 cccgacctgc cggagatcga cgactattac gagcccttcg acttcgacta cgggcatctg
     2461 caatccgcgc cggagatgga gcatttcccg acggcccggc cgcgctcgaa gatcaccggc
     2521 gagcggatcg agaagatcga atggggtccg aactgggagg agatcctcgg gggcgagttc
     2581 gagaagcggt cgaaggacta caatttcgag ggcatccaga aggagatcta cggccagttc
     2641 gagaacacct tcatgatgta tctgccgcgg ctctgcgagc attgcctcaa cccgagctgc
     2701 gccgcctcct gtccctcggg cgccatctac aagcgcgagg aggacgggat cgtcctgatc
     2761 gaccaggaga aatgccgcgg ctggcggatg tgcgtctcgg gctgccccta caagaagatc
     2821 tactacaact gggacaccgg caagtccgag aaatgcacct tctgctatcc gcgcatcgag
     2881 agcggccagc cgacggtctg ttcggagacc tgcgtcgggc gcatccgcta tctcggcgtc
     2941 atgctctacg acgcggacgc catcgagaag gcggccaatg ccgagaacga gaccgatctc
     3001 tatcaggcgc agctcgacat cttcctcgac cccaatgacc cggtggtcca ggagatggcg
     3061 ctggccgacg gcgtgccgca ggactggctc gaagccgcga agcgctcgcc ggtctggaag
     3121 atggcgatgg aatggaaggt cgccttcccg ctgcaccccg aataccgcac cctgccgatg
     3181 gtctggtacg tgccgccgct ctccccgatc aagaatgcgg ccgaggccgg gctgatcggg
     3241 cacgatcgcg agatgccgga cgtgcgcagc ctgcgcatcc cgctgcgcta cctcgccaat
     3301 ctgctgaccg cgggcgacga ggcgcccatc gccacctgcc tcgagcggat gctcgccatg
     3361 cgcggctaca tgcgggcgaa gaccgtggac ggggtgatcg acgaaggcat cgcggaacgt
     3421 gtcgggctca cgcccgccca gatcgaggac atgtaccgga tcatggcgct cgccgattac
     3481 gaggaccgtt tcgtcatccc gaccgcccac cgcgagcttg ccgaggagga catcatgggc
     3541 ctgcgcggcg gctgcggctt caccgacggc aacggctgct cggacggtct ctccaagggc
     3601 tcgctcttcg gcaaccggcg ccgggggctg accattccgg aggcgatgaa gtcatgaccc
     3661 tcacgctcaa ggcgctctcg gcgctgctaa cctatccaga cgcggcgctc gcgcggggca
     3721 ccgccgagat cgagacagcg ctgcgcgacg aggccctgct cgacgcctcc gccatcgagg
     3781 gtctgcgccc gctgctggcg gagctcggca gccgggacct cttcgacctg caggagcgct
     3841 tcgtgctgct cttcgaccgc tcgcgctcgc tctcgctcaa cctcttcgag catgtgcacg
     3901 gcgaaagccg cgcccggggg caggcgatgg tcgacctgct cgagacctat cgcgccggcg
     3961 gcttcgagcc ggtcgggccg gagctgcccg atcacctgcc gatgctgctc gagttcctcg
     4021 ccgcgcgccc gctcgacgag gcgcgcgagg tgctggccga caccgtgcat atcctggtca
     4081 cgctccgcga ccgcctcgac cggcggcaga gcccctatgc ccctgtcttc gccgccctgg
     4141 tgtccctggc cgccgcgccg ccggccgccg aggccctcgc cgagatcgcg gcgcatcccg
     4201 aggacgaccc cgaggatctc gccgcgctcg atgccgtctg ggaggaggcc gcggtgacct
     4261 tcggcccgga cccaaacgcc ggctgcccgg ccggccggga gatgctttcc cgcatgggcc
     4321 cgccggcaca gccgccggcc tgggccggtg gcggctcctg atcgcggagg acgcaagatg
     4381 aacttcttcc tgttccaggt ctatccctac atcgccctga ccgtgctcgc gctgggctcg
     4441 atcgcgcgct acgagaccga tcccttcacc tggaaatcgt cctcgagcca gctcctgcgg
     4501 cggcgccagc tgctgctcgg ctcgatcctc ttccatgtgg gcgtgctgat cgtcttcctc
     4561 ggccatttcg tcgggctcct gacgccgatc ggcatcttcg atgcgctggg catcggccat
     4621 accttcaagc agctgctggc gatgatcgtc ggcggcgtcg cgggcgtctt cgcgctggcc
     4681 ggcggggtga tcctgctcca tcgccgcctc tacgacccgc ggatccgggc gacctcgagc
     4741 ttcgccgaca tcggcatcct ggccctgctc gacctccagc tcgtgctcgg gctcctcacg
     4801 atcgtcgtgt cggcccagca tctcgacggc ggggagatgg tccgcttcat gggatggacg
     4861 caggcgatct tcaccttccg caccgatgcc tggctgctgg tggcggaggc ctcgtggatc
     4921 ttcaagctcc acatcttcct cggcctgacg atcttcgtgc tcttcccctt cacccggctg
     4981 gtgcacatgc tgtccgtgcc ggtgcgctac ctctggcggc ccggatacca gatcgtgcgc
     5041 tcgcgcaagg ccggcccggc gccggcggcg cggccgcatc ccgcggagtg accggatgcg
     5101 cggccccctg cagccggacg tggtggtcaa tgccgagcgg atccccgcgc ggctgatcgc
     5161 ggcggaagcc cagaaccatg ccgcgccgcc cggcaagccc ggccatgcct ggcgggccgg
     5221 ggcccgcgcc ctcgcggtgc gcgcgctcct gctgcaggag gcgcggcgcc tcggcctcgc
     5281 gccggagccg cgggacctcg gcgcgggccg ccgggaggtg cccgaagagg cgctgatccg
     5341 cgccgtcatc gagcggcgca tgcagccggt cccgcccgac gaggacgcct gtcgcgcatt
     5401 ctatgaccgg cacgcggcac atttccgcgc gccggccgtc tacgaggcct cgcatatcct
     5461 gatcgccgcg ccggaggatg ccgacgcccg cacccgggcg gatgccgccg cccgcaccct
     5521 aatcggcgtc ctggcgacgc gcccgcagga cttcgcggac atggcccgcc ggcattcgga
     5581 ctgcccgtcc agggcgaacg gcggccgcct cggccagctc tgcgccggcg acacggcccc
     5641 ggaattcgag gctgctctcg gcggtctcgc ggtgggagag atcacccccg agccggtcgc
     5701 gacgcggtac ggcagtcaca tcattcggct cgatgcgcgc gagcagggcg cgatcctgcc
     5761 ctacgacctg gcccggccac gcatccgcga gacgctcgaa cgcctcgcct ggtcgcatgc
     5821 cgcgaaatca ctggtcgcgg ccctcctcga agacgcgacc atcaccggcg tcgatttcga
     5881 ccatgcggcc tgaacccgcg gtgccctgac agcgacggtg cttccgtccg accggcgccg
     5941 cgacccgatg tcgacgctcg ggcaaggaaa aaggccgcgc cccgcgcggc ccgatcgcca
     6001 cgggtcgatc gggctaggga tcctcgaccc cgggggaagc aaagtcgatg gcgagggccg
     6061 ctggtatcag ctgcctcgcg ctttgtgccg ttgttgcaat gcagcgtggg ccggaggcgt
     6121 gtttagcctc cggcccggcc gcgggggggc ggccctccag cgccagtccg gccagtccgg
     6181 gcgcgggaga cgtcccgcta cgtcagcgct tcgtcgaaga ggtgtgccat tgccgggtcg
     6241 aaggacaatc cgatctcgga tcccggctga agcacgcggt cctcggcgat cgctgcgatc
     6301 acctggcttc cgtcccgcaa ggtgacgtcc accactgtct cgcttcccag ccgctcggtc
     6361 agggccacgg tgccgtgcag catgccctgg tccggtgcgg tgggttgcat gtactggggc
     6421 cggatcccca gggtcgcctt gccgcccttg acgatcggtc gccccttggt cccgaccgag
     6481 atcgggtcga gcgagccatt ggcgatggtc gcgccctcgg cgcccatgtc gcgcacgtcc
     6541 acggtcatga agttcatcga cggcgccccg agaaagcccg cgacgaagag gttcgcggga
     6601 ttgtggtaga gctccatcgg cgagccgatc tgcatgacct cgccgccgcg caggacgacg
     6661 atcttgtcgg ccagggtcat ggcctccacc tggtcgtggg tcacatagat catcgaggcg
     6721 ttgagcgact ggtggagctt gccgatctcc atccgcatgt ccatgcgcag cgccgcgtcg
     6781 aggttcgaga gcggctcgtc gaagaggaac accgaggggt cgcgcacgat ggcgcggccg
     6841 atggcgacgc gctggcgctg cccgcccgac agctgcgagg ggcgccggtc gagcaggtgc
     6901 tccatctgca ggatgcgggc ggcctcggcc accttccgct ccttctcggc cttgggcgtg
     6961 cggctgagcg tcaggccgaa ggccacgttc tcgcgcaccg tcatgtgcgg gaagatggcg
     7021 taggactgga acaccatggc gatgccgcgg tccttcgggg gaaggtcgtt gacgacacgg
     7081 ccgccgattt ccagcgtgcc cgacgagatg tcctccagcc cggcgatcat ccgcagcagg
     7141 gtcgacttgc cgcagcccga ggggccgacg aagacgacga actcgccctc gtcgatgtcg
     7201 atgttcacgc ccttgatgac ctggacctcg ccgtagttct tgcgaacgtc cttcagtctg
     7261 atggttgcca ttgcgcttcc tcctcactcg gccgcaagct gcgcgccctg gacgcggagc
     7321 attccgtccg catcgatttc caccgggtcg ggatccgtga cctggcccac gaactggccc
     7381 gccgggtaat cggcgaaacc gatgatcttc agccccgcat ccgtcttgag gatgcgcgcg
     7441 gcgtagcggc ggcagggcag ggcgccgtcg aggaaatgcc ccggggcgat ccgccacggc
     7501 cctcgggggc cctccccgat caggtaatgc gagcctgaga ccgggccgcc gggatgggtc
     7561 tccgcctggt ccttggagaa atgttcggcg gcggtgcaga acaggcagta ccagcggccg
     7621 ccctgctcga agacctgcgg tacctccagc tggccgtagc cgccgacgaa gaccggcggc
     7681 tcgaggaccc agtcaaagag gtcgggcgag gtcgcgaagc cgatggcgcc gccggcattg
     7741 ggctcgttca cccccgcggc gcgggcggtg aagaacatca gccagccgtc gccgttggga
     7801 tcggccatga cccaggggtc gcgcatcgcc cggtcgtgcc agaagccgtc ctggaaggag
     7861 gcttcgtagc tggcggcatg gggaccgacc aggtcgaggc agggcgcctc gcccacccgc
     7921 ttccagttgt gcatgtcggt cgagaccgcg tggccgatgc gctggtagag cccgtcctcc
     7981 gcccgcgagg cgccggtata gaagaggtgc catgtcccgt ccggcccctg caggaccgat
     8041 cccgtccagg tggtgcaatc gtcccagccc gggccttcgg agggctcgag gcaggtgccg
     8101 agatgggtcc agctgaccag gtcgtcgctg accgcgtggc cgtggcgggc gttgcggtgc
     8161 cggagctgcg gatccccgag gcttttcggc gccttgagga agtagccgtg ccagtgttcg
     8221 ccgtcatggg cgtaccagct gtcccagatc caatgcgtgt cgagtgcaag aaccatggtg
     8281 ccctccgtta tcccttcaca ccggtcgagg cgatcgacgc gatgaaggcg cgctgcagga
     8341 cgagatagaa ggccagcacc gggatggtga tcagcgagag ataggccatg atctcgcccc
     8401 aggcggtgtt gagctggaag aagtactgca gcccgaccat gaccggccgg taggcctcgt
     8461 cctgaaccac gatgaggggc cagagatact ggttgtacat caccaggaac ttgaggatgg
     8521 cggcggtggc gaggaccggc ccggccaacg gcatgaccac cttcaggtag atctgcaacc
     8581 aggtcgcgcc ctcgacccgg ctggcctcga tcagctcccg cggcaggtcc ttgaagtact
     8641 gcacgaagag aaagatcgtc aggccgtcgg ccgcccaggg caggatctgc acgcggtagg
     8701 tgttgagcca gccgtgctcg atccccgaga acccgatcca gggcagcttg gagaccagaa
     8761 gcagcagcgg gatcgcgatg gtctcgaacg gcacgatcag cgtcgcgagg atgatcgaga
     8821 gcacgaccgc ccggccgcgc cattcgagga agacgaaggc gaaggcggcg agcgagcaga
     8881 ccagaaggca gatcagcacc gtcagcccgg tgacgaagac ggaattgaac acgaacaatc
     8941 ccaccggcgc ccgctggaag gcatgggtgt agttgctgag cccgatgtcg ccgaccggca
     9001 ggaaggcccg gaggctaccc gtgtcgctca gcaactgctg gtcgggcttc agcgaactga
     9061 cgaccatgaa gaccagagga aagatgaaga tcaccgcgac gagaacgagc acgaggtagc
     9121 ggacggcgag gcgcagcccg tggttgtcct gggaaatggt cgccatcacg ttctctcccg
     9181 tgtcagccag cgttgaatga gcgagatgca gagcacgatc aggaagagca ggaccgagat
     9241 cgtcgagccg cccgagatgt cctgcttgcc gtagccgcgc tcgaccgcct ggaacacgag
     9301 cgtctgcgtc gaatccagcg gcccgccctt ggtcatcacg tcgatctgcg cgaagagcgc
     9361 gaagcattgc atggtgatga cgatcaggat cagcacggcg gtattgcgaa gcccgggcca
     9421 ggtgacgaag cggaaccgct gccacttggt cgcgccctcg atatcggccg cctcgtagag
     9481 cgtcggcgaa atcgtctgca gcccggcgag ccagatcacc atgtgaaagc ccaccgcctg
     9541 ccagatcgac atgacgatga tcgatcccag cgccgtggag ggattgccga gccagtcgac
     9601 cggctggaac aggccgaagg tcaggccgga caggaggttg ttcaagagcc cgtcctggcc
     9661 gtcgtagatg aaccgccaga gcagcgagac gacgacgatg gagacgacca ccggcatgaa
     9721 atagatgccg cggaagacgt tgatgccccg cagccgctgg ttgatgagca gggccagccc
     9781 gagcgccagc gcggcctgac acggcgccac gaccagaacg aaggtgaagg tgttgaccag
     9841 cgccttcatg aagacgacgt cgcgggcgat caccacgcgc gcgtcgtcgc ccgaggacca
     9901 ccggaaccat tcgcgcatgc cgcgatactg cggcgtgtcg tcgttgcgcg tgaccgagcg
     9961 caggctcgga tattcgatgt tcccgtcctc gtcccgcagc accgcgccgt cgtcgccccg
    10021 ctccggctcc agcgtgacca ccgccacgcc gagcaggctg cggtaattgt cgagcccgac
    10081 gaattcggtg gggttgggcg agatcaggcg ctggttggtc agcgacaggt agaaggcgaa
    10141 gaagaaaggc aggacgatga aggtgccgat cagggcgaag gcgggaatcg caaagcccca
    10201 ccccgctctg tcggagcgca gcagactgga tgccattggt agttcctccc ggagggccgg
    10261 ggcgtgcgtt gccgccccgg ccgggtttgc gtcagtttcc gtagccgctg ttggcctcga
    10321 tatcggcatc gatctcgtcg gccgccgcat ccagcgtgtc ggccacgtcg gcgccgctgg
    10381 cgatatccgc cagggccttg cggaagatct tggccatgac cggatagccg ggggtcaccg
    10441 gccgcagcag ggcctgttcc ttgctgaggt catagaacac ggccatcggc gcgccctcgg
    10501 catagagctc gctttccgcc gcggcggagc tggtggccgg aatgacggcc tgcgcattgc
    10561 tgaaggccac ttgccagcgg tcctgcaaca tgaactcgat gaactggttc gcgccttccg
    10621 gatggtcgga cgaggccgag acggcgaatt gccaggacgc cgcgccgatc ttcggaccat
    10681 tgccaaaatc cggcgccggc aggaacagca cgtcgtcgcc gaaggcttcc accgcattgg
    10741 ccgcggccca gtttccgttc cagctcatcg cgtatttgcc gtcgatcagg ccggaatccc
    10801 ggtccgccgg atcctggctg gtgccgggca ccagttcgcg gtcgaacagc gactgccacc
    10861 actcgccgaa ttcgatgccg gcatcgccgt tcagcacgcc gtccgattcc agataggtgc
    10921 tgcggtcgat caggtcgccg ccgaagctct gcaggaaggg ggcgaaggca taggggtacc
    10981 attcgtccgc gctccaggcc atgccgaggt cgagcggata gtcgaattcg ccgccttcct
    11041 ggatcgcctc cagcgcggca tcgagctcgt cgcgggtcca gggctcggcg agggtcggga
    11101 tgcggatgtc atacttctcc agcaccgacc gccgggtgac catggcgacg gcggcttccc
    11161 acaggccgac cgaatagatg tcgtccttcc agcggccgat ggccgtcggc aggaagttct
    11221 cgatcgtgcc ttggtcgatt tccagaggtt ggatgtagcc ggcccatgcc cagctcggca
    11281 tgaccgggcc gtccacatcg atgatgtcgg gcaggttgcc cgccagcgcg ccggcgatga
    11341 cggtgtcgtt gtaggcgccg gccgggaagc tctgcacgac caccgaccag tcggactggc
    11401 tggcgttgaa ctcctcgacg atctggtcga gcacagcgct ttcttccggg ttgcccgcgc
    11461 cgtgatacca catggtcagc tcggtctggg cgaaggcctg cgcgccccag atcgcgccca
    11521 gtgcggtggt ggcgaatgcg gtcagacgta tgttcatgcc gggttcctcc ctctcattgg
    11581 caattcggat atgcggttcg cggggccggg ccggacgctg tcgcgccaga ccgcggggcc
    11641 gccgaccaga accggtgctt ccggcgcggc gttgccgtcc cggaccatgg acgtgagcag
    11701 ctgcgcggcc ctgacgccca tggcggcata gggcagcttc accgtggtca gcttcggata
    11761 gagcgtctcg gtgatcagcg tgtggtcgtc gtagcctgcg accgagatgt cgtcgggcag
    11821 cgtcaggccg cgggagcgca gcatgccgta ggcgcgcagg gccatgcggt cgttgccgag
    11881 gcagagaacc gtcggcggtt cctcgagcgc cagcacccgg tcgagcgcgt cccagagcaa
    11941 ctgctcttcg gccagatggt cgtccgccga gaggttcgcc gccgtgacca gggccgggtc
    12001 gaagggaatg ccggcgatgg ccagcgcgtc gcggtagccg gcgacccgca agcgcgtcgc
    12061 ttccatctcg gggccgagcg tcagataggc gatcctgcga tgcccgcgcg cgatcagctc
    12121 ctccgtcagc gccttctggc cggagcggtc gttgggcagt accgccgggc tgcccgcatc
    12181 gtcgaagcaa ttggccagca ccagcttcgt gtcgccggcg atcccgggca gggtgacctt
    12241 catgtggtgg tcggcgacga agatgatccc ctcgacccgg tgctcctgga aggtgcggac
    12301 caggttggga atgcgttcgg acttgccgcc ggtatcggag atgaggagcg tcagcccgct
    12361 ctcctcgagc tcccgcagga tgccctggac gatctcgatg tccggcaggc cgccgagcgc
    12421 catgcgcctc ggggtcaggg tgatcgcccc ggtgatcagg ccgaccaggc cggagcggtt
    12481 cgaccgcatg gtgcgggcgg ccgaggaggg gacatatccc agctcggcga tcgcgcgctc
    12541 gaccttgtcc cgggtggatc ggccgaccgg cgcatcgccg ttgagaaacc ggctgacggt
    12601 cttgggcgag accccagccc gcttcgccac gtcgtagatc gttgacacag gcccacctcc
    12661 ccccggagcg attcgatttt tgacatcgat gtcatgacac cgatgtcata ctcggtcggc
    12721 cagttcaaga atgaagttcg ggggggaaga cggcggccgt ggtggcccat acagcggctc
    12781 ctgctccggc tcggccggca gcatgcagaa gatcggccga ccgaagggcg cacctccgac
    12841 cgggccgggc agccccgcgc cgacgccgat tggacagcgc cagcctgccg ccgaaccggt
    12901 gcctgccggt gccgcatacg agatcctgca cccgaaactg gaccgtggat tgatgcggtc
    12961 acaatgccag cggtgggacg acttcccgac gcacattttg aataaagtgt tgtataacaa
    13021 tattttacat agcgtttcat cgtgaaacgc cgctcctgac cgaccgcgac ccggccaagg
    13081 ccaaggcctt catcgacttc cagaacgacg tgacggccaa ggacatccgg cttgcggtcg
    13141 gcggccacgc cgaaggctgg agcctccgcc tcgaggccga cgtcgcg
//
LOCUS       DAOTFI010000139        13005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5728_length_13005_cov_6.404402, whole genome shotgun sequence.
ACCESSION   DAOTFI010000139 DAOTFI010000000
VERSION     DAOTFI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 13005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13005
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5728_length_13005_cov_6.404402"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1351)
                     /gene="metZ"
                     /locus_tag="P5558_14550"
     CDS             complement(167..1351)
                     /gene="metZ"
                     /locus_tag="P5558_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HRY25583.1"
                     /translation="MAEDGAQGWRLETQLVQGGLDRSHHGETAEALYLTSGFVYERAE
                     HAEDRFRDRAPGFMYSRYGNPTVSTFEQRLAAIEGAEACCAVGSGMAAVNAAFMCRLK
                     AGDRVVAARALFGSCRHIIANILPRFGIEHEFVDGADLEQWRQALSRPTALVFFETPS
                     NPTLELVDIAAVAELAHQAGAEVVVDNVFSTPLLQRPHALGADVVVYSATKHIDGQGR
                     CLGGAILGTQRYRDDTLHPYLKHTGPSLSPFNAWVLLKGLETLPLRVAQQCRSAQQIA
                     EHLEAHPKVRRARYPGLESHPQYALARRQMQAGSTLVAFHVGGTKERAFEVLNRLRLA
                     VISNNLGDSKTMVTHPATTTHSKLPPEERRALGITDDLVRLSIGLEHVDDIIADLDQA
                     LA"
     regulatory      complement(1397..1476)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(1527..2582)
                     /locus_tag="P5558_14555"
     CDS             complement(1527..2582)
                     /locus_tag="P5558_14555"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRY25584.1"
                     /translation="MEGLARRLLHIPPRLLASLPPETAHDLAIRLIPLAPRGRQAERP
                     GLETTIAGIDLAHPLGLAAGFDKDARAALGLLRLGFAFVEVGTVTIRPQAGNPRPRLF
                     RLAADRAIINRMGFNNAGIEAMAGRLQGRPHTGITGVNIGINKDSTDPARDYGIAAAR
                     LLPLADYLTINVSSPNTPGLRDLQEERALAGVLDAVLEARRNAGPAKPVFLKVAPDLD
                     DRSADAITETAMARGIDGIIATNTTIARPPGLRSPARAEKGGLSGRPLMAPSTRLLQR
                     MARRAAGRLAFIGVGGIASGADAYEKVLNGASAVQLYTAFVYEGPAIIDRILDELEAC
                     LARDGHADLASAIGTGL"
     gene            complement(2591..3358)
                     /gene="cysQ"
                     /locus_tag="P5558_14560"
     CDS             complement(2591..3358)
                     /gene="cysQ"
                     /locus_tag="P5558_14560"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRY25585.1"
                     /translation="MVDPKRHLPELIRLADAAAGEIMRHYGRDTAVDYKADRSPVTLA
                     DEAGEAIILEGLRALTPDWPIVAEEAVSRGEIPAVDDGPFWLVDPLDGTKEFINQRGE
                     FTVNIGLVQARLPVMGVVFAPAIDTMWWGAVGHGAERRKAGVTEAIAVRRAPPAGGPV
                     AVASRSHRDAETDAWLAEHGITETLSCGSSLKFCAVAEGRADVYPRFGPTMEWDTAAG
                     HAVLRAAGGEVVTVAGQPFTYTKPDFRNGGFIARAHG"
     gene            3660..5021
                     /locus_tag="P5558_14565"
     CDS             3660..5021
                     /locus_tag="P5558_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25586.1"
                     /translation="MPASFALHPPRTAHGLDPRHPGRFAERIEAFRRHHAALPAPRLA
                     GGLQLLDIEELRLRFGNRWPAVREKAYQIVEGSLTKRLGAHDLYVAVESDRFHLLVTD
                     IDRLAAERRGRFIAAEITERLCGMVPGGVACKLQTTGFDLVQGLARITGLGELEARIR
                     DFRRKVDDAEEALFAAHEPEIQALYQPILHFRKRLVTGYALTPMLDGETGRQGVAELC
                     PSSLNGVFDAALDRWSIAQVAPHLHAARTALRLTLHYSTLASMRHREPLVLACRRLPA
                     GADRRLIIEIAGLPASLPQARVRELVSYLRPFAVAILVRIDADLLAAAGSNGGPPDRL
                     PMVTDNLQASGVAGISALLRAARNGTDDVGEGGPLDRAAVAGDAGYGAAVLGGLGVLG
                     RAAGLRTCAFIETRVEAGAGAGAATGIGPARAAIAAGIDYLCGEALLPPTLRPGRVVA
                     LSG"
     gene            5250..6791
                     /locus_tag="P5558_14570"
     CDS             5250..6791
                     /locus_tag="P5558_14570"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRY25587.1"
                     /translation="MSEARPEAPVPATSAATPAGRQRPLGEGEGPERGEPLSAVRLGA
                     LVLGPLLAILIVLLPAPEGMPDPAWKLVSMTVWMVVWWLGEAVPIPATALLPIPLMPL
                     YGISAMGPVAASYADSLIFLFLGGFLLAAAMQRWGLHRRIALRIVAAVGTSPGGIIGG
                     FMLATGFLSLWISNTATTVMMFAVGLSVIDFIGRQTKDDRMLRNFGVALMLGIAYSAS
                     IGGVGTLIGTPPNALLASFLASTYDIQITFFTWMLFGMPVVIVMLPLAWLLLTKLIFP
                     ARKLQVGDASGVVRGELLALGSMGYAEKVVGGVFLGAALAWIFRSPIVALTGLPINDT
                     SVAMIAALVLFAWPVSRAEGTFVLDWSAARNVPWGVLLLFGGGLALAGGFKETGLAEW
                     IGNSVTGFDVGTWVLVLIVVTAIVYLTEITSNTASTATFLPILGAVAVGLELDPRMLA
                     VPVAVGASMAFMMPVATPPNAIVFAYERMQLRDMVMAGFWLNILAIAVTFGAMYLLAG
                     AVFGI"
     gene            complement(6801..7592)
                     /locus_tag="P5558_14575"
     CDS             complement(6801..7592)
                     /locus_tag="P5558_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HRY25588.1"
                     /translation="MPEIDPALRLAAAEAVAREAGSLARRYYLVRDTLEIESKGAQDL
                     VSQADREVETLIRDRLAKLFPEDGFVGEEHAHALKTDGGGHWVIDPIDGTWCFLNGIG
                     SWCVSLAYERAGKVETGVVYDPMADELFAARRGGGAVLNGVPIRVADATSLADGTVSL
                     GYSLRVPVTSMLPLFDKLLNARGMYQRHGSGALGLAWTAAGRLIGYLEPHMNSWDCLA
                     GLLLVEEAGGFTNDFLENNGLLDGNPVIAGPKALEAEFARLTDHW"
     gene            7687..8511
                     /locus_tag="P5558_14580"
     CDS             7687..8511
                     /locus_tag="P5558_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY25589.1"
                     /translation="MIIVEANGARMPAIGFGTWDLPQERVQALVEHAIATGYRHVDTA
                     QMYKNEREVGAGIKGSGVKRDDIFLTTKIWPDQFRSHELMRAADERLKLLGVDAVDLL
                     LLHWPAKDVPLAETIEALNAIRESGKARHIGVSNFTAAMVDEAVRLSAAPLVTNQVEY
                     HPYLSQTKVMTALRRHGMALTAYCPIARGKVLGDPVIGAVAEAHGRSATQVTLRWLVQ
                     QEGVVAIPRSSNEARITENLGVFDFALSDAEMSRISALGSPTGRLVNGAFAPAWDA"
     gene            8508..9179
                     /locus_tag="P5558_14585"
     CDS             8508..9179
                     /locus_tag="P5558_14585"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRY25590.1"
                     /translation="MNPVSFQAVEEAALLRLGSAALQARLAVPKSAAELEATGDDRYL
                     SSMSLRLFRAGLKHELVDRKWPAFEEVFLGFDPARVAGLSDEALEALLQDKRLIRHMG
                     KLRAVRANAGEMLAIAEEHGSFARWLARWPVGEITGLWASLAKRMQHLGGQSAPYFLR
                     MVGKDTFVLTDSVARGLAYWEVRDGPPRGKAEIAALQAVFNRWQEETGRPLCQLSQIL
                     ALSID"
     gene            9189..9935
                     /locus_tag="P5558_14590"
     CDS             9189..9935
                     /locus_tag="P5558_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25591.1"
                     /translation="MSPVDFPAGGYRYLPGVSQYSGGVVAVAGHEIVRVRFRRRLPLA
                     EGFARAKAIIEAAGRPLAAFCACELRSPAPFTEEGFRQFNAAYVARLESWGIVRDGVN
                     PVARSNLCPEIDPPAEPSFHAFCFTTEAESDAPSFVISGSGEVPEGQGNYRDHVIRPG
                     DTSPGGMAEKARFVLAEMERRMAALGFAWPDTTASQAYTVFDLFPFFAEEIVRRGAAA
                     HGLTWFYERPPVVGLDFEMDCRGVGREGVV"
     gene            10030..11205
                     /locus_tag="P5558_14595"
     CDS             10030..11205
                     /locus_tag="P5558_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25592.1"
                     /translation="MTNDPFFSPGDNWEWNACIGDQGDEQYYINGYIEAAIELVDAIF
                     EKKLYGKRDTLVLPILYNARHAIELALKMAIRELKGMGVLNSAPLKNHDILAHWRHLD
                     SSNLGDEWLKKIVAEIKPFVLSLAQIDEDGQEFRYHETRDGDRSLRGQSVANLAVIRE
                     SLNLLQEALKSLMHRLYCLDSERKGGFFTPDLSRNDLVRIASSLPDRDDWNTPQFDCV
                     KEDNMIRYSIGARKYAEALKLIQQNRETRGLIGMETVLRYLSDDKARLLTEKHLALWP
                     PPKEGSKPRIIKASEINYEAMTQASGRERGAIQELLDQLSCQEIADVSVAFHIGLLHE
                     IPEIYEVLLDTKLETLKTRKSAEAELIHVFRKSNFRVDLAHGLQRLGRIQLARELLR"
     gene            complement(11253..11870)
                     /locus_tag="P5558_14600"
     CDS             complement(11253..11870)
                     /locus_tag="P5558_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY25593.1"
                     /translation="MTAAAATLLDFLTLSEALKRELRHSWLSDGRQESVAEHSWQMAL
                     FAILAHPHLEEPVDLGKTLEMITVHDLVEALVGDVPYFETGERKRLKKQREAEAIEDI
                     RTRLGPPTGDRVRDLFLEFEDRQTPEARFAGAIDNLEVQIQHNLADFSTWTEAEYGLI
                     YDKLDTPCAHDPFLKALAESVRQRALAKMRAGGVEVLCTHWGPVI"
     gene            complement(11891..>13005)
                     /locus_tag="P5558_14605"
     CDS             complement(11891..>13005)
                     /locus_tag="P5558_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRY25594.1"
                     /translation="LSRTGEVVEQGRVSKILAFRGLDRLPVDEAEAGDIIAIAGLTKA
                     TVADTLADPAVEAPIAAQPIDPSTIAMTFSVNDSPYAGQDGTKVTSRQIWDRLVREAE
                     GNVAIRISRSPENDAFEVAGRGELQLGVLIETMRREGFELSISRPRVLFQEDPETGKR
                     LEPIEEVVIDVDEEYAGAVVEKLTRRKGDLTEMRPAGAGKQRLVFHCPSRSLIGYHGE
                     FLTDTRGTGVMNRLFFAWQPYRGPIAQRHTGVIVSGEQGEAVGYALWNLEDRGSMFVE
                     HGTKVYSGMIIGEHNRGNDLEVNPLKGKKLSNVRAAGKDDAILLTPPVRLTLEQAIAY
                     IADDELVEVTPKYIRLRKRILDPSERKKAARRPEVA"
BASE COUNT         1991 a         4071 c         4753 g         2190 t
ORIGIN      
        1 cacgccatgg cgcgcagcgt aagcagcttg acaggccaag acagtcgctg accccattgc
       61 acccgtattg agtcctgacc ccatttcacc tcgcgatcgc gaaagacatc gccatccttc
      121 gaatttccgt ccttctcggc actttcttca cgccggccgt tcaatctcac gccagcgcct
      181 ggtcgagatc ggcgatgatg tcgtcgacgt gctcgaggcc gatggagagg cggacgaggt
      241 cgtcggtgat gccgagggcc cggcgttcct cgggcggcag cttcgagtgg gtggtggtgg
      301 cggggtgggt gaccatggtc ttgctgtcgc cgagattgtt ggagatgacg gcgaggcgga
      361 gcctgttcag gacctcgaag gcgcgttcct tggttccgcc gacatggaag gcgacgaggg
      421 tcgagccggc ctgcatctgg cggcgggcga gggcgtattg ggggtggctt tcgaggccgg
      481 ggtagcgggc gcggcggacc ttcgggtggg cttcgaggtg ctcggctatc tgctgggcgc
      541 tccggcattg ctgggcgacg cggagcggca gggtctcgag gcccttgagc agcacccagg
      601 cgttgaacgg gctgaggctg ggtccggtat gcttgaggta ggggtggagg gtgtcgtcgc
      661 ggtagcgctg cgtgccgagg atcgcaccgc cgaggcagcg gccctggccg tcgatgtgct
      721 tggtggcgga gtagacgacg acgtcggcgc cgagggcgtg cgggcgttgc aggagaggcg
      781 tggagaagac gttgtcgacc acgacctcgg ccccggcctg gtgggcgagc tcggccacgg
      841 cggcgatgtc gacgagctcg agggtcgggt tggagggggt ctcgaagaag acgagggcgg
      901 tgggccggga cagggcctgg cgccactgct cgaggtcggc gccgtcgacg aattcgtgct
      961 cgatgccgaa gcgcggcagg atattggcga tgatgtggcg gcaggagccg aagagggccc
     1021 gggcggcgac gacgcggtca ccggccttca ggcggcacat gaaggcggcg ttgaccgccg
     1081 ccatgccgga gccgacggcg cagcaggcct cggccccctc gatggcggcg aggcgctgct
     1141 cgaaggtgct gaccgtgggg ttgccgtagc gggagtacat gaagccgggg gcacggtcgc
     1201 ggaagcggtc ctcggcatgc tcggcgcgct cgtagacgaa gcccgaggtg aggtagagcg
     1261 cttcggcggt ctcgccgtgg tgcgagcggt cgagcccgcc ctgcaccagt tgcgtctcca
     1321 gccgccagcc ctgcgccccg tcttccgcca tgttcctgct ccgcaagcaa aaaaccccgg
     1381 ccggaaaagg ccagggtcca catgacgccc ggccttttag cggattgttt aacgtggccc
     1441 gcaagccgac cggctcaaat caccacaggt cggcgattag gcgcgatcgc cggcggcgtc
     1501 aagcggggtg tgccggctgg cgccgctcag agtccggtgc cgatggccga ggcgaggtcg
     1561 gcatggccgt ccctggcgag gcaggcctca agctcgtcga ggatccggtc gatgatggcg
     1621 gggccctcgt agacgaaggc ggtgtagagc tggacggccg aggcgccgtt caggaccttc
     1681 tcgtaggcgt ccgcacccga ggcgatgccg ccgacgccga tgaaggcgag gcggccggcc
     1741 gcgcggcggg ccatgcgctg caggaggcgg gtggaaggtg ccatgagcgg ccggccgctg
     1801 aggccgccct tctcggcgcg cgcgggcgag cgcaggccgg gcgggcgggc aatcgtggtg
     1861 ttggtggcga tgatgccgtc gatgccgcgg gccatggcgg tctcggtgat ggcgtcggcc
     1921 gagcggtcgt cgagatcggg ggcgaccttg aggaagaccg gctttgccgg gccggcgttc
     1981 ctgcgggcct cgagcacggc gtcgagcacg cctgccaggg cgcgctcctc ctgcaggtcg
     2041 cgcaggcccg gcgtgttggg cgaggagacg ttgatcgtca ggtagtcggc gagcggcagc
     2101 aggcgggcgg cggcgatgcc gtagtcgcgg gcggggtcgg tcgaatcctt gttgatgccg
     2161 atattgacgc cggtgatgcc ggtgtgcggg cggccctgga ggcggccggc catggcctcg
     2221 atgcccgcgt tgttgaagcc catgcggttg atgatggcgc gatcggcggc gaggcggaac
     2281 aagcggggac ggggattgcc ggcctgcggg cggatggtga cggtgccgac ctcgacgaag
     2341 gcgaagccga ggcgcaagag gcccagggcg gcgcgcgcgt ccttgtcgaa gccggcggcg
     2401 aggccgagcg gatgggcgag gtcgatgccg gcgatggttg tctcgagccc agggcgctcg
     2461 gcctgccggc cgcgcggcgc cagcggaatc aggcggatcg ccaggtcgtg cgcggtctcg
     2521 ggcggcaggc tggcgagcag ccgcggcggg atgtgcagga gccgccgggc caggccttcc
     2581 atcgaaggaa tcagccgtgg gcgcgggcga tgaagccgcc gttgcggaag tcgggcttgg
     2641 tgtaggtgaa gggttggcct gccaccgtga ccacctcgcc gccggcggcg cgcagcacgg
     2701 cgtggccggc cgccgtgtcc cattccatgg tcggcccgaa gcgcgggtag acgtcggccc
     2761 ggccctcggc gacggcgcag aacttgagcg agctgccgca ggagagggtc tcggtgatgc
     2821 cgtgctcggc gagccaggca tcggtctcgg cgtcgcggtg ggagcggctg gcgacggcga
     2881 ccgggccgcc ggccggcggg gcccggcgca cggcgatggc ttcggtgacc ccggccttgc
     2941 ggcgctcggc gccatggccg acggcccccc accacatggt gtcgatggcc ggggcgaaga
     3001 cgacgcccat gaccggcagg cgggcctgga cgaggccgat attgacggtg aactcgccgc
     3061 gctggttgat gaactccttg gtcccgtcga gcgggtcgac cagccagaag gggccgtcgt
     3121 cgacggccgg gatctcgccc cggctcacgg cctcctcggc gacgatcggc cagtccgggg
     3181 tgagggcgcg caggccctcg aggatgatcg cctcgccggc ctcgtcggcg agggtgaccg
     3241 gcgagcggtc ggccttgtag tcgacggcgg tatcccggcc gtagtggcgc atgatctcgc
     3301 ctgccgcggc atcggcgagg cggatcagct cgggcagatg ccgcttcggg tcgaccatgt
     3361 ggggctccag ctctctcggg gatcggcggc cggcgggtgt cgaccacggg gccggtgcta
     3421 gcgaaccggg ggagcggaaa caagcacgcg ggcgccgcac ccgggcgtgg tcgaccgccc
     3481 gagtgtcgtt aacgggatct caaccaggtg gtgctagctt cagcatcgac gaggccggcg
     3541 agcgtgccgg ccccccgacc ctggccggag cgatgctgca gcgggcagtc gccgatcgac
     3601 ccgttgtgaa ggcgcccccg gcggcaggtg ccgcgcctgc gcggccttcg ccggcggcga
     3661 tgccggcgtc gttcgcgctc catccgccac ggaccgccca cgggctcgat ccgcgccatc
     3721 cgggccggtt cgccgagcgt atcgaggcgt ttcgccgcca tcatgcggcc ctgccggcgc
     3781 cacgcctggc cggcgggctc cagctcctcg acatcgagga attgcgcctg cgcttcggca
     3841 atcgctggcc ggcggtgcgc gagaaggcct accagatcgt cgagggcagc ctgacgaagc
     3901 ggctcggcgc gcatgatctc tacgtcgcgg tcgagagcga ccgcttccat cttcttgtca
     3961 ccgacatcga tcgcctggct gcggagcggc gcggccggtt catcgccgcc gagatcaccg
     4021 agcggctgtg cggcatggtg ccgggcgggg tcgcctgcaa gctgcagacg accggcttcg
     4081 atctggtcca gggcctggcc cggatcaccg gcctcggcga gctcgaggcc cgcatcaggg
     4141 atttcaggcg caaggtcgac gatgccgagg aggcgctctt cgccgcgcac gagcccgaaa
     4201 tccaggcgct ctaccagccg atcctccatt tccgcaagcg gctggtgacc ggctatgcgc
     4261 tgacgccgat gctggatggc gaaacaggcc gacagggcgt tgccgagctc tgtccctcga
     4321 gcctcaacgg ggtcttcgac gcggctctcg atcgctggtc gatcgcgcag gtggcgccgc
     4381 atctgcacgc cgcgcgcacg gcgctgcggc tcacgctgca ctactcgacc ctcgcctcga
     4441 tgcgccaccg cgagccgctc gtcctcgcct gccgccggtt gccggccggc gccgaccggc
     4501 gactgatcat cgagatcgcc ggcctgccgg cgagcctgcc gcaggcccgg gtgcgcgagc
     4561 tggtgagcta tctgcgaccg ttcgcggtcg ccatcctggt ccggatcgat gccgatctgc
     4621 tcgccgccgc gggatcgaac ggtggcccgc cggacaggct gccgatggtc accgacaacc
     4681 tgcaggcgtc gggcgtggcc ggcatttcgg cgttgctgcg ggccgcccga aacgggaccg
     4741 acgacgtcgg cgagggcggt cccctcgacc gggcggcagt cgccggcgac gctggttatg
     4801 gcgcggcggt gctcggcggg ctcggggtcc tcggacgagc agccggcctg cggacctgcg
     4861 ccttcatcga gacgcgggtc gaggcaggtg ccggggccgg agccgcgacc gggatcggcc
     4921 cggcacgcgc cgccatcgcg gctggcatcg actatctctg cggcgaggcg ctcttgccgc
     4981 ccacgctccg gccagggcga gttgtcgcct tgtctggctg aaaggcgccg acgatggaga
     5041 gcggaggatg agaagctgac ggcgggattg tccgcaaggg cgtcacgggc aatggtgacc
     5101 gaccattgca gggcaaggca gtcggtgggc cgattagggg tggtatggct cgagcggctg
     5161 cgccggccgg cgccgcgccg tggcgcttgc cagcgccgtc gggcggggac aagctgcagt
     5221 tgaaggccga agcaggtcgg gggaggccaa tgagcgaggc gcgtccggaa gccccggtgc
     5281 cggcgacgag cgctgcgacg cccgccggtc ggcaaaggcc gctgggcgag ggcgaggggc
     5341 cggagcgcgg cgagccgctt tcggccgttc ggctcggtgc cctggtgctc ggcccgctcc
     5401 tggccatcct gatcgtgctc ctgccggcac ccgaaggcat gccggacccg gcctggaagc
     5461 tcgtctcgat gacggtctgg atggtggtct ggtggctcgg cgaggcggtg ccgatcccgg
     5521 cgacggcgct cctgccgatc ccgctgatgc cgctctatgg gatctcggcc atggggccgg
     5581 tcgcggccag ctatgccgac agcctgatct tcctctttct cggcggcttt ctgctcgccg
     5641 ccgccatgca gcgctggggc ctgcaccggc gcatagcact gcgcatcgtg gcggcggtcg
     5701 ggaccagccc gggcggcatc atcggcggct tcatgctggc gacgggcttt ctctcgctct
     5761 ggatctccaa caccgcgacc acggtgatga tgttcgcggt cgggctctcg gtcatcgact
     5821 tcatcggccg ccagacaaaa gacgatcgca tgctgcgcaa tttcggcgtg gcgctcatgc
     5881 tggggattgc ctacagcgcc tcgatcggcg gcgtcggcac gctgatcggc acaccgccca
     5941 acgcgctgct cgcgagcttc ctcgccagca cctacgacat ccagatcacc ttcttcacct
     6001 ggatgctgtt cggcatgccg gtggtcatcg tcatgctgcc actggcctgg ctgctgctca
     6061 ccaagctgat cttcccggcg cgcaagctgc aggtgggcga tgcgagcggc gtcgtccgcg
     6121 gcgagctttt ggccctcggc tcgatgggct acgccgagaa ggtggtgggc ggcgtcttcc
     6181 tcggggcagc gctcgcctgg atcttccgct cgccgatcgt ggcgctgacc ggcctgccga
     6241 tcaacgacac ctcggtggcg atgatcgccg ccctcgtcct cttcgcctgg cccgtgtcgc
     6301 gcgcggaggg caccttcgtg ctcgactgga gtgccgcgcg gaacgtgccc tggggcgtgc
     6361 tgctgctctt cggcggcggg ctggcgctgg ccggcgggtt caaggagacg ggcctcgccg
     6421 agtggatcgg caactcggtc accggcttcg acgtcggcac ctgggtgctg gtgctgatcg
     6481 tggtgacggc catcgtctac ctcaccgaga tcacctccaa cacggcctcg accgccacct
     6541 tcctgccgat cctgggcgcc gtcgccgtgg gccttgagct cgacccgcgc atgctggccg
     6601 tgccggtggc ggtgggggcc tcgatggcgt tcatgatgcc ggtggcgaca ccgcccaacg
     6661 ccatcgtctt cgcctacgag cggatgcagc tcagggacat ggtcatggcc gggttctggc
     6721 tgaacatcct ggcgatcgcc gtgaccttcg gcgccatgta cctgctcgcc ggcgccgtgt
     6781 tcggcatctg agcgccgggc tcaccagtgg tcggtcagcc gggcgaactc ggcctcgagc
     6841 gccttcggcc cggcgatcac ggggttgccg tcgagcaggc cgttgttctc gaggaaatcg
     6901 ttggtgaagc cgcccgcctc ctcgacgagg agcagcccgg cgaggcagtc ccagctgttc
     6961 atgtgcggct cgagatagcc gatcagccgt ccggcggcgg tccaggcgag ccccaaggca
     7021 cccgagccgt gccgctggta catgccgcgg gcgttcagca gcttgtcgaa gagcggcagc
     7081 atggaggtga cgggcacgcg cagcgagtag ccgaggctga ccgtgccgtc ggcgagcgag
     7141 gtggcgtcgg ccacccggat cggcacgccg ttcagcaccg ccccgccgcc gcgccgggcg
     7201 gcgaagagct cgtccgccat ggggtcgtag acgacgccgg tctcgacctt gccggcgcgc
     7261 tcgtaggcga ggctgacgca ccaggagccg atgccgttga ggaagcacca ggtgccgtcg
     7321 atcgggtcga tcacccagtg cccgccgcca tcggtcttga gcgcgtgcgc gtgctcctcg
     7381 ccgacgaagc cgtcctcggg gaagagcttg gcgagccggt cgcggatcag ggtctcgacc
     7441 tcgcggtcgg cctggctcac caggtcctgg gcacccttgc tctcgatctc gagcgtgtcg
     7501 cggacgaggt agtagcgtcg ggcgaggctg ccggcctcgc gggcgacggc ttcggcggcg
     7561 gcgagccgca gggcggggtc gatctcgggc atcattcggt ccgggcttgg ggagcaggtt
     7621 gccgcgggtt ataggacggg acggacgccg cgacgagagc ggggcgaaaa aatcggaggg
     7681 acgcgcatga tcatcgtcga ggcgaacggg gcgaggatgc ccgccatcgg cttcggcacc
     7741 tgggacctgc cgcaggagcg ggtgcaggcg ctggtcgagc atgccatcgc caccggctac
     7801 cggcatgtcg acaccgccca gatgtacaag aacgagcgcg aggtgggggc cggcatcaag
     7861 ggctccggcg tcaagcgcga cgacatcttc ctcaccacca agatctggcc cgaccagttc
     7921 cgcagccacg agctgatgcg cgccgccgac gagcggctga agctgctcgg cgtcgatgcg
     7981 gtcgacctgc tcctgctgca ctggccggcc aaggacgtgc cgctcgccga gaccatcgaa
     8041 gcgctcaacg cgatccgcga atccggcaag gcccggcata tcggtgtgag caacttcacg
     8101 gcggccatgg tcgacgaggc cgtgcggctg agcgctgcac cgctcgtcac caaccaggtc
     8161 gagtatcacc cctatctcag ccagacgaag gtcatgacgg cgctccgccg ccacggcatg
     8221 gcgctcaccg cctactgccc gatcgcgcgc ggcaaggtgc tgggcgaccc ggtgatcggc
     8281 gccgtggccg aggcgcatgg ccgctcggcg acccaggtga cgctgcgctg gctggtccag
     8341 caggaaggtg tcgtcgccat tcccaggagt tcgaacgagg cgcggatcac cgagaatctc
     8401 ggcgtcttcg acttcgccct ctcggacgcg gagatgagcc ggatcagcgc cttgggctcg
     8461 cccacgggaa ggctggtgaa cggcgccttc gcgcccgcct gggacgcatg aacccggtca
     8521 gcttccaggc cgtcgaggag gccgccctcc tgcgcctggg ctcggcggcg ctgcaggcga
     8581 ggctggccgt gccgaagagt gcggccgagc tcgaggccac gggcgacgac cgctacctct
     8641 cttccatgtc gctccggctc ttccgggccg ggctcaagca cgagctggtc gaccggaagt
     8701 ggcccgcctt cgaggaagtt tttctgggct tcgacccggc ccgggtggcg gggctctcgg
     8761 acgaggcgct cgaggcgctc ttgcaggaca agcggctgat ccgccacatg ggcaagctcc
     8821 gggcggtccg ggcgaatgcg ggggagatgc tggcgattgc cgaagagcac ggcagcttcg
     8881 cccgctggct cgcccgttgg ccggtgggcg agatcaccgg gctctgggcc agcctcgcca
     8941 agcgcatgca gcatctgggc ggccagtcgg caccctattt cctgcgcatg gtcggcaagg
     9001 acacgttcgt cctgaccgat tccgtggcgc gcggcctcgc ctactgggag gtcagggacg
     9061 gcccgcccag gggcaaggct gagatcgccg ccctgcaggc ggtcttcaac cgctggcagg
     9121 aggagaccgg ccggccgctc tgccagctct cgcagatcct ggcgctgtcg atcgactgag
     9181 gaggcacgat gtcacctgtg gattttccgg cgggcggcta tcgctatctg ccgggggtga
     9241 gccagtattc gggcggtgtg gtggcggtgg ccggccacga gatcgtgcgc gtgcgctttc
     9301 gccgaaggct gccgctcgcc gaaggcttcg cccgggcgaa ggcgatcatc gaggccgccg
     9361 ggcggccgct cgcggccttc tgcgcctgcg agctccgctc gcccgccccc ttcaccgagg
     9421 agggctttcg gcagttcaac gcggcctatg tggcgcggct cgaatcgtgg ggcattgtcc
     9481 gggacggggt caacccggtg gccaggagca atctctgccc cgagatcgac ccgccggcgg
     9541 agccctcctt ccacgccttc tgcttcacca ccgaggcgga gtcggatgcc ccgagcttcg
     9601 tgatctcggg cagcggcgag gtgccggagg gccagggcaa ctaccgggac cacgtcatcc
     9661 gcccgggcga cacgagcccg gggggcatgg ccgagaaggc ccgcttcgtc ctcgccgaga
     9721 tggagcgccg catggccgcc ctcggtttcg cctggcccga cacgacggca agccaggcct
     9781 acacggtctt cgatctcttt ccgtttttcg cggaggagat cgtgcggcgc ggggcagcgg
     9841 cgcatgggct gacgtggttc tacgagcggc cgccggtggt ggggctggac ttcgagatgg
     9901 attgccgggg agtgggaagg gagggggtgg tgtagtctcg gaccaaagaa attcgaagcg
     9961 ccgttgccac atatgaaata tgtcctggac ccatttttct ccgctaaaac ttaaagaaag
    10021 cccagttcat tgactaatga tccatttttt tcacctggag ataattggga gtggaatgct
    10081 tgtattggcg atcaaggtga cgaacaatac tatatcaacg gatatatcga agctgcaatc
    10141 gaactcgttg acgcaatatt tgaaaaaaag ctctacggta agagagatac gttggtcctg
    10201 cccatcctat ataacgcgcg acatgccatc gagttagcat tgaagatggc gattcgcgaa
    10261 cttaagggta tgggtgtcct caatagcgca ccacttaaga accacgatat tcttgcgcat
    10321 tggcgtcatt tagacagttc taatttaggt gatgaatggc tcaagaaaat tgtcgcggag
    10381 atcaaaccct tcgtcttgag tctggctcaa atcgacgagg atggtcaaga attcagatat
    10441 cacgagacac gcgatggcga tcggagtctt cgggggcagt ctgtagcaaa tctcgccgtt
    10501 atccgcgaat ctctcaactt actgcaagaa gctctgaaat ccctgatgca tcggctgtat
    10561 tgcctcgaca gcgagcggaa gggtggattt ttcactcctg acctctcacg caatgaccta
    10621 gttcgaattg ctagtagcct gccagacaga gatgattgga acacgcctca atttgattgc
    10681 gtaaaagaag acaatatgat cagatactcc attggcgctc gcaagtacgc agaagcgctg
    10741 aaacttattc aacaaaatag agagacaaga ggtctaattg gaatggagac cgttctgcgt
    10801 tatttgtctg atgacaaagc gcgcttgtta acggaaaaac acctagctct atggccaccg
    10861 ccaaaagaag gctcgaaacc tcgcataatt aaagcatctg aaataaatta tgaggcgatg
    10921 acccaggctt ccgggcgaga aaggggcgct atccaggagc ttcttgatca actttcttgc
    10981 caagaaattg ccgatgtcag cgttgcattc catatcggcc tgctacatga aatacccgag
    11041 atctatgaag tgctacttga taccaaattg gaaacactta agactcgtaa gagtgctgag
    11101 gcagagctta tacatgtatt tagaaagtct aatttccgag tagacttggc gcatggtctt
    11161 cagaggcttg gaagaataca gcttgcaagg gaattgctac gttgagacta ttgaagttta
    11221 aaaaacatcg ccgctgccta aaggcggtgg tttcagatga cggggcccca atgtgtacag
    11281 agcacctcca ccccacccgc ccgcatcttc gccagcgccc gttggcgcac cgattcggcc
    11341 agcgccttga gaaacgggtc gtgggcgcag ggggtgtcga gcttatcgta gatcaggccg
    11401 tactcggcct cggtccaggt ggagaagtcg gcgaggttgt gctggatctg gacctcgagg
    11461 ttgtcgatgg cgccggcgaa gcgggcttcg ggggtctggc ggtcctcgaa ctcgaggaag
    11521 aggtcgcgga cgcggtcgcc tgtgggtggg ccgaggcggg tgcggatgtc ctcgatggcc
    11581 tcggcctcgc gctgcttctt gaggcgcttc ctttcgccgg tctcgaaata gggaacatcg
    11641 cccaccagcg cctcgacgag gtcgtggacg gtgatcatct cgagtgtttt gccgagatcg
    11701 accggctcct cgaggtgggg gtgggcgagg atggcgaaga gcgccatctg ccaggaatgc
    11761 tcggcgacgc tttcctggcg gccgtcggag agccagctgt gacggagttc gcgtttcagg
    11821 gcttcgctga gcgtcaggaa gtcgaggagc gtggcggcag cggcggtcat gcagggccgg
    11881 cgcccctgga tcacgccacc tcggggcggc gggcggcttt cttgcgctcg ctggggtcga
    11941 ggatgcgctt tctgaggcgg atgtatttgg gggtgacctc gaccagctcg tcgtcggcga
    12001 tataggcgat ggcctgctcg agggtgaggc ggacgggcgg ggtgaggagg atggcgtcgt
    12061 ccttgccggc ggcgcggacg ttgctcagct tcttgccctt gagcgggttg acctcgaggt
    12121 cgttgccgcg gttgtgctcg ccgatgatca tgccggaata gaccttggtg ccgtgctcga
    12181 cgaacatcga gccccggtcc tcgaggttcc agagggcgta gccgacggcc tcgccctgct
    12241 cgccgctgac gatgacgccc gtgtggcgct gggcgatggg ccccctgtag ggctgccagg
    12301 cgaagaagag gcggttcatg acgccggtgc cgcgggtgtc ggtgaggaac tcgccatgat
    12361 agccgatgag cgagcgggac gggcagtgga agacgaggcg ctgcttgccg gcgccggcgg
    12421 ggcgcatctc ggtgaggtcg cccttgcggc gggtcagctt ctcgacgacg gcaccggcat
    12481 attcctcgtc gacgtcgatg acgacctcct cgatcggctc gaggcgcttg ccggtctcgg
    12541 ggtcctcctg gaagaggacg cgggggcggc tgatcgagag ctcgaagccc tcgcggcgca
    12601 tggtctcgat gagcacgccg agctgcagct cgcctcgccc cgccacctcg aaggcgtcgt
    12661 tctcgggcga gcggctgatg cggatggcga cgttgccctc ggcctcgcgc acgaggcggt
    12721 cccagatctg gcgcgaggtg accttggtgc cgtcctggcc ggcatagggg ctgtcgttga
    12781 ccgagaaggt catggcgatg gtcgagggat cgatcggctg ggcggcgatg ggtgcctcga
    12841 cggcggggtc ggcgagcgtg tcggcgacgg tggccttggt cagcccggcg atggcgatga
    12901 tgtcgccggc ctcggcctcg tcgaccggca ggcggtcgag gccgcgaaag gccaggatct
    12961 tggagacgcg gccctgctcg acgacctcgc cggtgcggct cagcg
//
LOCUS       DAOTFI010000140        12992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5735_length_12992_cov_6.728144, whole genome shotgun sequence.
ACCESSION   DAOTFI010000140 DAOTFI010000000
VERSION     DAOTFI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5735_length_12992_cov_6.728144"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..785)
                     /locus_tag="P5558_14610"
     CDS             complement(216..785)
                     /locus_tag="P5558_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRY25595.1"
                     /translation="MTETTCPRLIAAAFVAFTLPFAATQAIAAGSHGGGHGHAVHGEP
                     GDPAKASRTVTVEMDDNYYEPERIAVKAGETVRFTVTNAGGFVHEFNIGTPEMHAAHQ
                     DEMAMMVEHGVLLPDRIDQAAAAAMQASMGHGQHDDPNSVLLEPGQTADVVWTFPEDG
                     VIEFACNVPGHYDAGMAGRFDFVETLAQH"
     gene            complement(991..1215)
                     /locus_tag="P5558_14615"
     CDS             complement(991..1215)
                     /locus_tag="P5558_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25596.1"
                     /translation="MACAVRSILTSLLVLVVATMLAIEPARAHDGHTAVDVQATQHGA
                     WEPLDQHDHGGAPHDRSCVSMIGHCGTMPA"
     gene            1318..2217
                     /locus_tag="P5558_14620"
     CDS             1318..2217
                     /locus_tag="P5558_14620"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HRY25597.1"
                     /translation="MTSGQPRSPVATGAVQWPLAMAVLPAVAGCFLALARTTLAEMVE
                     PDSEPLWEIGVVGGGGYLPDYPAADENHVNGLALPFAIYRGEVFRLGDRGAARGIVVD
                     DGGFELDLGLDAAFPVDSDNSDAREGMNDLDFLLEVGPRLTYRFLPPEDRDELDLALA
                     VRAVLSTDFSNLRYQGLTVAPSLTYRRNDFLLDDMRAVVSVGPLFGFDGLNDYFYRVA
                     PSEARPGRPAYEADDGYMGTELSAGLSWGIRENLRLFGGVQLGYWGGSANDDSPLHRQ
                     DLTIGVGGGLRWSILTSERRVAR"
     gene            2300..4030
                     /locus_tag="P5558_14625"
     CDS             2300..4030
                     /locus_tag="P5558_14625"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY25598.1"
                     /translation="MSAGTSASSESGSLDVAILVADAVGYSRAMSRDEAAALGAFGAS
                     RRLIDPLIRQHGGRIFATAGDSILAEFGCADGAVGCAVAIQRGIETALAAGALLMRFR
                     IGVHLGPVHRVETDLLGATVNIAARLEGLSNTGGICISAAVRDALGSLDGLVLEPVGQ
                     RILKNIEAPIEVLRIRLGEPGREDFLEQRRLGVAVLPFTDPSGEVAGYLADGLADDLI
                     AGLARFRTLAVMGRGATFHYRGAAVDPRRVAADLGVGFVVRGTCVAEGAALRIGIELV
                     AAGTGPVLWAERFEGTASDLLATQDTIVERVVAALAGRIAAAGQADSIRRRPASPAAF
                     DLVLQGIFHAHQTEPAATATALRLFDEALALEPDYPLALAWQALMQLRRWGWAPTGGG
                     DLQPVLERARRAATLDPGDGWCQLVLGQVAMYAGDLDLAAVHHKRATALNPFDAHILA
                     LRSPLAVYLGKPEEGVLLIERALRLDPLPPPWYASNHGLALYAARRYAEAAAAYAAAA
                     MPQTGVLAGLAASLARLGDAAGARQAAARLMAREPHFRVDVFMTMRPFREAADRRHLR
                     EGLRLAGLPG"
     gene            complement(3966..4751)
                     /locus_tag="P5558_14630"
     CDS             complement(3966..4751)
                     /locus_tag="P5558_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25599.1"
                     /translation="MSPAAPLVEARGVTRVLAGAVPVTLVADIDLAIGRGELTAITGP
                     SGSGKSSLLYLLGLLDLPTTGEVLIDGRETSAMAENERAEFRLATLGFVFQFHFLLPE
                     FSACDNIMIPMRRLGRLDAAARRERAESILASLGLAEHGHKRPDQLSGGQRQRVAVAR
                     ALANDPPLILADEPTGSLDSQSSEQVFDILKTLVERDGRTVVAVTHDLDLADRMHRRI
                     HLVDGRITEDRPAGGRASPGPTISPAGRRGAGPPADAADQRLP"
     gene            complement(4748..5986)
                     /locus_tag="P5558_14635"
     CDS             complement(4748..5986)
                     /locus_tag="P5558_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25600.1"
                     /translation="MRLLLAIALTHIAGRGRQTIVSILGVALGVGFSIAMAALMVGSQ
                     RDFTETLIDAVPHVRITDETRTTPRQPASEAFAAVAYSGLRPVPDPRGILNPTRVLAG
                     LRSWVDGSMVATLQVEAVVRFAGIDMGVALMGVEPLDELRVSTIAGDMTTGRFEDLIG
                     AGFAVIIGERLAERLGAGINDLLTITVAGGNARQFKVTGLFRTGSVQQDERVVYTTLK
                     RAQVLANRPEAINEIRIRLADIDSAPLVAARAEAMLGIKSVSWQESSEDLLNAFEIRN
                     VIMFTVVGAILLVAGFGIFNIVSIITHEKARDIAILKSIGFPPADIRGIFLFEGLVMG
                     GSGALLGCLLGFGLTEVLGSIAFEFRRNTELTHLPVVYEAAHYVMASALAMLAAGIAG
                     YLPARSAARLNPVDIIRGAT"
     gene            complement(5983..6984)
                     /locus_tag="P5558_14640"
     CDS             complement(5983..6984)
                     /locus_tag="P5558_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY25601.1"
                     /translation="MRKLLLLAALATALALGVWWWLQRPLPVTTAVIARGSAAEVIYA
                     TGLVEPDRWAEVTSLARARIVDTCRCEGRFVELGAVLFRLDDSAAMAHVAELEAQLDL
                     ARKDLQRASDLQRRGIATEERVDQATAAVSQHSAAVTRARSELRDYVIVAPMAGQVLR
                     LDGEIGEVVTPGEPLAWVGQPQPLLVNASVNEEDIPRVALGQRALLKADAFPGRPLDA
                     TVHAITPMGDPELKTYRVRLALPDDTPLFIGMSVEVNILVRTVEATLLAPAPAVVEGA
                     VFVLGPDGRARRQPVATGIVGAEAVEITGGVEAGATVVSPVPAGLGDGARIAVRGPG"
     gene            7147..8187
                     /locus_tag="P5558_14645"
     CDS             7147..8187
                     /locus_tag="P5558_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY25602.1"
                     /translation="MRKTPIHTPDSRRSFIQKAALSSAAAGAMLGGFGFDPFIASAMA
                     QEMGRSEKPLKAAFSNAGLQATWCAQGKQAAEFWGKLFNVEVTWFDGELSAPKQRAAI
                     DNMATQDWDFVAIQAFGIGTLTDPVNKMIDRGIPVIDMDTLIAPLEEINVHSFLAPDN
                     EFMGASVTQALVDAIGGEGTMVMTQGALGHTGAQGRARGFFSVIEKYPGIEVLDEQPA
                     DWDVTKASRIWDSLLTKHPNISAAFFHNDDMALAAYNVMQARGRTDILIGGVDAMPPA
                     VDAVLEGHMYATVRNPSCRIHGGSIVAGVAAVLTGEKTGDGIPKHIITDGPVVTKANA
                     PGMLWMQKHFLI"
     gene            8196..9704
                     /locus_tag="P5558_14650"
     CDS             8196..9704
                     /locus_tag="P5558_14650"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY25603.1"
                     /translation="MSDGSERPPLIELSGIGKTFGGVTALSNVDFVLREGEVHGLVGE
                     NGAGKSTLMKIIAGVHTEFEGSYRLDGQPVRLRSTRDALARGIGMVHQELSIVPHLSV
                     AENVFLGNQPVSSLGVVRWRHMAREAEAHLKNLGIEVDPTVEAGSLSLGLQQLIEIGR
                     VLFSGARIIILDEPTSALSPPEVERLFQVIDRLRQGGKSFIFISHFLDDVLAISDTVS
                     IFRNSRHVRTAPAAELDKGQIIELMIGRGHEELEESYLGTLELPPASTAPTLLEVRGL
                     TLKGAFEELDLAVHEGEALGIYGFMGCGQLELVRALVGKLKADRGTLVIGGVERRLAN
                     TAAGKRAGVALVPESRRSMLFADSPIYRNISISVLERISRWLVKPVAERQLAREKIEE
                     LRIRAGSETVALRTLSGGNQQKVALARWLAYRPNVLVLAEPTRGMDVGAKDDVVKIIR
                     ALKEQKMGVIVVSTEPETVLSLADRILVMRRGRFTREFVAEAVGKDQLLAAA"
     gene            9732..10703
                     /locus_tag="P5558_14655"
     CDS             9732..10703
                     /locus_tag="P5558_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25604.1"
                     /translation="MKVWVLRRLTAIAPLITLLFLVAIFVSASPSFATIDNAANILRQ
                     VSITAIIATGLTFVILTAEIDLSVASVANAVGILVAFFTLQAAYVNIANVPLPGGFAI
                     LFAIMAALALGGITAFGVVVIGIPSFIMTLAMMQIGAGISALLVRGQIAYNVPDLVKT
                     LGSGSIAGIPWIVIVATLFLAVGHFVLSYTRFGRYVYMVGGNREAAEYAGVNVKLIVA
                     AVMIIGAFCSGVAGMLGVAYYGSAQQNQFDDFLLDSIAAVVVGGTSLFGGRGGIPNTI
                     IGLFVLGVLNNGLDHVDIDSFLKILIRGLILLLALVINVYAQKIRAD"
     gene            complement(10713..12845)
                     /locus_tag="P5558_14660"
     CDS             complement(10713..12845)
                     /locus_tag="P5558_14660"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRY25605.1"
                     /translation="MRWILYALLLIVGLPTVLLAGAWLALRQGWLNGVIEARASAATG
                     YDIELGDAPDLAWKDGALRLTLGPLAIANSEDEELATVDRAAAELALGPLLHGDIVLP
                     NVEVEGVDAEVVRGEDGTLNWQPAERPTPSTDGPPIPPLIRHLVVEDAQIDYRDGERD
                     VTLVLDELEGNWGATEPLTLTGAGRLGDAPLSIEGSGPSRQALVDGADEPLQATLDAA
                     STSITAAMQPAAGTFSLDLEAGDAFADFLATLGLAVPTLPAFELDLEGELQGEGAVVD
                     ARLTIDRTTLQFDGSVDHFGAATALHGTFRAAGPNPGPILASLGLAQIDTPPYDLEGE
                     LTREGSVLTLDGISGTIGDSDIAGSLRIDRAAQPLAVEADLRSRSLDFDDLFPIVGLP
                     PQTGAGETASPEQRQEAQTLDQRPRLIPDFRIDPAIWRGIDLDIELDAAEVRSELFPI
                     DRLAIHLVTREGWITIDPFTTGLADGRLVLFFSFDTTQRPPVGRVDLRLTNLSLHDML
                     ARIGEANEAAGRVDGRVRLEGRGYSLADLLGSSDGQVGLVMSDGSLDAFLVEAVGLDL
                     MEGLITLITPDDAANRVPIRCGVANLQVARGIATARPLLLDTADTKLTAAGTIDLEAE
                     TMDLLIEAHPKDPSLLSGNQPLQVEGDWRSPTIAPAPGEVESNTLGWLLAPIAALVPF
                     FDLGLAEDAPCGQLLDEARKAAAEAPDE"
BASE COUNT         1858 a         4365 c         4756 g         2013 t
ORIGIN      
        1 tgcgggtgaa gtcgccggtc tcgatcgtga cgcggtcgac ggtgaggtcg taggtggcgg
       61 cggtcgcggg tatgtcgaag gccacgagcg tggcgagcgc cacggccgcg gcagcggtcc
      121 gatgtccgat catgggcgta tccatggcga tgtagtcgac gggtggggtg ccgcgatggt
      181 cgcggcacac gctcgttttc cgtgtgttct ggccgtcagt gctgggccag ggtctcgacg
      241 aagtcgaacc tgcccgccat gccggcgtcg tagtggccgg gcacgttgca ggcgaactcg
      301 atcacgccgt cctccggaaa ggtccagacc acgtccgccg tctggccggg ctccagaagc
      361 acgctgttcg ggtcgtcatg ctggccatgg cccatcgaag cctgcatggc ggcggctgcc
      421 gcctggtcga tgcgatcggg caggagcacg ccgtgctcca ccatcatcgc catctcgtct
      481 tggtgtgcgg cgtgcatctc gggcgtgccg atgttgaact cgtgcacgaa gccgccggcg
      541 ttggtcaccg tgaagcgcac ggtctcgccc gccttcacgg cgatccgttc gggctcgtag
      601 tagttgtcgt ccatctcgac ggtgacggtg cgggaggcct tggcaggatc gcccggctcg
      661 ccatgaacgg cgtggccgtg gccgccgccg tggctgccgg cggcgatggc ctgggttgcg
      721 gcgaagggta gcgtaaaggc gacgaaggcg gcggcgatca ggcgcggaca ggtcgtctcg
      781 gtcatgtctg ttcctcgaag gtctggatcg gtcgcaagtg aggccgtccg cgccacggat
      841 ctggcgcgaa gagccggctc ggcggcaagt ccgtcagact cgaggagggg gtgtgtccgc
      901 ttcgggcgag cgtccgacga aggcgcggcc gtccggcggc agcatggcag cctgcgtcgc
      961 catgggcagc accagggcaa cggcgacgtc ctacgccggc atcgtgccgc aatggccgat
     1021 catcgagacg caggagcggt catgtggcgc accaccatga tcgtgctggt cgagcggctc
     1081 ccaggcgcca tgttgcgtgg cctggacatc gacagcggtg tggccatcgt gggcgcgtgc
     1141 cggctcgatc gccagcatcg tcgcgacgac gaggacgaga agcgacgtca ggatcgagcg
     1201 gacagcgcag gccatggttc agcgcgtaag ccttccattg ttggaaggtc aaggactatc
     1261 tgatcggtcg cgcggatcgt ccgcacgctg cttctttcga caggagagac catctcgatg
     1321 acttccggcc agcctcgatc gcccgtcgcc actggcgccg tacaatggcc gctcgccatg
     1381 gccgtcctgc cggcggtggc cggctgtttt ctggctctgg cccgcacgac cctggccgag
     1441 atggtcgagc ccgacagcga gccgctctgg gagatcggcg tggtcggcgg cggggggtat
     1501 ctgccggact acccggcggc cgacgagaac catgtcaacg ggctcgccct gccgttcgcc
     1561 atctatcggg gcgaggtctt ccggctcggc gaccgggggg cggcgcgcgg tatcgtcgtg
     1621 gatgatgggg ggttcgagct cgatctcggg ctggatgcgg ccttccccgt cgattccgac
     1681 aacagcgacg cccgcgaagg catgaacgat ctcgacttcc tgctcgaggt cgggccgcgc
     1741 ctgacctatc gcttcctgcc gcccgaggac cgggacgagc tggatctcgc gctcgccgtt
     1801 cgcgccgtcc tctccaccga cttcagcaac ctccgctacc agggcctgac cgtcgccccg
     1861 agcctcacct accggcgcaa cgacttcctc ctcgacgaca tgcgcgcggt cgtgtcggtc
     1921 ggcccgctct tcggcttcga cgggctgaac gactacttct accgcgtggc gccgagcgag
     1981 gcgcgcccgg gccgaccggc ctacgaggcg gatgacggct acatgggcac cgagctgagc
     2041 gccgggctct cctggggcat ccgcgagaac ctgcgcctct tcggcggggt gcagctcggc
     2101 tattggggcg gttccgccaa cgacgacagc ccgctgcacc gccaggacct gaccatcggc
     2161 gtcggcggtg ggctgcgctg gtcgatcctc accagcgagc ggcgggtggc gcggtaggcg
     2221 cctaggctgg ctggccaaaa cgggccgcga tagactggcg ccggcggccg ctcgcaaggg
     2281 ccgtggcggt gagcggatca tgtcggcagg caccagcgcg tcgtccgaaa gcgggagtct
     2341 cgacgttgcc atcctcgtcg cggacgcggt cggctacagc cgggcgatga gccgggacga
     2401 ggcggcggcg ctgggtgcct tcggggcaag ccggcggctg atcgacccgc tgatccggca
     2461 gcatggcggg cgcatcttcg ccacggctgg tgacagcatc ctcgccgagt tcgggtgtgc
     2521 cgacggtgcc gtgggctgcg cggtggcgat ccagcgcggg atcgagacgg cgctggcggc
     2581 cggggcgctc ctgatgcgct ttcgcatcgg cgtgcatctg gggccggtgc atcgggtgga
     2641 gaccgatctc ctgggcgcca cggtcaacat tgcggcccgg ctggagggtc tctcgaacac
     2701 cggtggcatc tgcatctccg cggcggtgcg cgacgcgctc ggctctctcg acggcctggt
     2761 gctcgagccg gtcgggcagc gcatcctcaa gaacatcgag gcgccgatcg aggtcctgcg
     2821 gatcaggctc ggcgagccgg ggcgggagga tttcctcgag cagcgccggc tcggcgtcgc
     2881 cgtcctgccg ttcaccgacc cctcgggcga ggtcgcgggc tatctcgccg acggcctcgc
     2941 cgacgacctg atcgccgggc tcgcccggtt ccgcacgctg gcggtgatgg gccgcggtgc
     3001 cacgttccat tatcggggag cggcggtcga cccgcgccgg gtggcggcgg atctgggtgt
     3061 gggcttcgtc gtccgcggca cctgcgtggc ggagggcgcc gctctgcgga tcggcatcga
     3121 gctggtcgcg gccgggaccg gcccggtgct ctgggcggag cggttcgagg gcacggcctc
     3181 ggacctgctc gccacgcagg acaccatcgt cgagcgggtc gtggcggcac tggccgggcg
     3241 gatcgccgcg gcagggcagg cggactcgat ccgccggcgg ccagccagcc cggccgcctt
     3301 cgacctcgtg ctgcagggca tcttccacgc gcaccagacc gagccggcgg ccaccgcgac
     3361 ggcactccgg ctcttcgacg aggcgctggc gctcgagccg gactatccgc tggcgctcgc
     3421 ctggcaggcg ctgatgcagc tccgtcgctg gggctgggcg ccgaccgggg gcggtgatct
     3481 gcagccggtg ctcgagcgtg cccgccgggc ggcgacgctc gacccgggcg atgggtggtg
     3541 ccagctcgtg ctcgggcagg tcgcgatgta tgcgggcgat ctcgacctcg ccgccgtgca
     3601 ccacaagcgg gcgacggcgc tcaacccgtt cgatgcgcat atcctggcgc tgcgctcgcc
     3661 gctcgccgtc tatctcggca agccggagga aggtgtcctg ctgatcgagc gggccttgcg
     3721 cctcgatccc ttaccgccgc cctggtatgc cagcaatcac ggcctcgcgc tctacgcggc
     3781 ccgtcgctac gccgaggcgg cggccgccta tgccgcggcc gcgatgccgc agaccggcgt
     3841 ccttgccggc cttgcggcga gtctcgcgcg gctgggtgac gcggcgggcg cgcggcaggc
     3901 ggcggcgcgc ctcatggccc gcgagccgca cttcagggtc gacgtcttca tgacgatgcg
     3961 ccccttcagg gaagccgctg atcggcggca tctgcgggag ggcctgcgcc tcgccggcct
     4021 gccgggctga tcgtcgggcc gggggaagct cgcccgccgg ccgggcgatc ctcggtgatg
     4081 cgcccgtcga cgaggtggat gcggcgatgc atgcggtcgg ccaggtcgag atcgtgcgtg
     4141 acggcgacca cggtgcggcc gtcccgctcg acgagggtct tcaggatgtc gaagacctgc
     4201 tcggaggact ggctgtcgag cgagcctgtc ggctcgtcgg ccaggatgag cggtgggtcg
     4261 ttggcgaggg cgcgggcgac ggcgacgcgc tggcgctggc cgccggagag ctggtccggg
     4321 cgcttgtggc cgtgctcggc gaggccgagc gaggcgagga tgctctcggc ccgctcgcgc
     4381 ctcgccgcgg cgtcgagccg gccgagccgg cgcatcggga tcatgatgtt gtcgcaggcc
     4441 gagaattccg gcagcaggaa gtggaactgg aagacgaagc cgagcgtggc gagacggaat
     4501 tccgcccgct cgttctccgc catggcactg gtctcgcggc cgtcgatcag cacctcgccg
     4561 gtggtcggca ggtcgagcag cccgaggaga tagaggagcg acgacttgcc ggagcccgag
     4621 ggcccggtga tagcggtgag ctcgccccgc ccgatcgcca ggtcgatgtc ggcaaccagc
     4681 gtgaccggca cggcgccggc gaggacgcgg gtgacgcccc tggcctcgac cagcggggca
     4741 gcggggctca agtggcgccc cggatgatgt cgacggggtt gagccgggcg gcgctgcgcg
     4801 ccggcaggta gccggcgatg ccggcggcca gcatggcgag ggccgaggcc atgacgtagt
     4861 gcgcggcctc gtagaccacc ggcaggtggg tgagctcggt gttccgacgg aactcgaagg
     4921 cgatgctgcc cagcacctct gtcaggccga agcccagaag gcagccgagg agcgccccgg
     4981 agccgcccat gaccagcccc tcgaagagga agatgccgcg gatgtcggcc ggcgggaagc
     5041 cgatcgactt gaggatggcg atgtcgcgcg ccttctcgtg ggtgatgatc gagacgatgt
     5101 tgaagatgcc gaagccggcg accaggagga tggcgccgac gacggtgaac atgatgacgt
     5161 tgcggatctc gaaggcgttg agcagatcct cgctgctctc ctgccaggag accgacttga
     5221 tgccgagcat ggcttcggcg cgggcggcaa cgagtggcgc cgaatcgatg tcggccaggc
     5281 ggatgcggat ctcgttgatg gcctcgggcc ggttggcgag gacctgggca cgcttgagcg
     5341 tggtgtagac gacccgttcg tcctgttgca ccgagcccgt gcgaaagagc cccgtcacct
     5401 tgaactggcg cgcattgccg ccggccacgg tgatcgtcag gagatcgttg atgccggcac
     5461 ccaggcgttc ggccaggcgc tcgccgatga tgacggcgaa gccggcgccg atgaggtcct
     5521 cgaagcgccc cgtggtcatg tcgccagcga tggtcgagac ccggagctcg tccaggggct
     5581 cgacgcccat gagggcgacc cccatgtcga tgccggcgaa gcggaccacg gcctccacct
     5641 ggagggtcgc caccatgctc ccgtcgaccc agctcctgag gccggccagc accctggtcg
     5701 ggttgaggat gccgcgcggg tccgggaccg gcctgaggcc cgaataggcg acggccgcga
     5761 aggcctcgct cgccggctgg cgcggcgtgg tgcgtgtttc gtcggtgatg cggacatgcg
     5821 gcacggcgtc gatcagggtc tcggtgaagt cgcgctggct gccgaccatg agggcggcca
     5881 tggcgatcga gaagccgacg ccgagcgcga cgccgaggat cgagacgatg gtctgcctgc
     5941 cgcgaccggc gatatgggtg agggcgatgg cgagcaggag cctcatccgg gcccccgcac
     6001 ggcgatccgc gcaccgtccc cgaggccagc cggcaccggc gagacgacgg tcgcaccggc
     6061 ttcgacgccg cccgtgatct cgaccgcctc ggcgccgacg atgccggtcg cgaccggctg
     6121 gcggcgggcg cgtccgtcgg ggccgaggac gaagacggca ccctccacca cggccggggc
     6181 cggggcgagg agcgtcgcct cgaccgtgcg cacgaggatg ttcacctcga cggacatgcc
     6241 gatgaagagc ggcgtgtcgt cgggcagcgc caggcggacc cggtaggtct tgagctcggg
     6301 gtcgcccatg ggcgtgatcg cgtgcacggt ggcgtcgagc gggcggcccg gaaaggcgtc
     6361 ggccttgagg agggcccgct ggcccagggc gacgcgcggg atatcctcct cgttgaccga
     6421 ggcattgacc aggagcggct gcggctggcc gacccaggcc aggggctcgc ccggggtcac
     6481 cacctcgccg atctcgccgt cgaggcgcag cacctggccg gccatgggtg ccacgatcac
     6541 gtagtcgcgc agctcgctgc gcgcgcgggt gacggcggca ctgtgctggc tgaccgccgc
     6601 cgtcgcctgg tcgacgcgct cctcggtggc gatgccgcga cgctggaggt cgctcgcccg
     6661 ctgcaggtcc ttgcgggcga ggtcgagctg cgcttccagc tcggccacat gcgccatggc
     6721 tgccgaatcg tcgaggcgga agaggacggc gccgagctcc acgaagcggc cctcgcagcg
     6781 gcaggtatcg acgatgcgcg ccctggccag cgaggtcacc tcggcccagc gatccggctc
     6841 gaccaggccg gtggcgtaga tcacctccgc ggcgctgccc cgggcaatca cggccgtggt
     6901 gacgggcagg gggcgctgca gccaccacca gacgccgagc gccagggcag tggcgagggc
     6961 cgcgaggaga aggagcttgc gcatgctgtt ggctgcccgg cagttccggt tgaaggcctc
     7021 gatgtcgccc ctggcgggta tagcgcaagt cggctgttca tcgcgcttgc atccggtggc
     7081 ttcagacagg aatatccgcg cggtcgccgg gttggcaccg gcgtgcaacg atcagggagg
     7141 caagtcttgc gcaagacacc cattcacaca ccggactccc ggcgctcctt catccagaag
     7201 gcggccctgt ccagcgctgc cgccggcgcc atgctcggcg gcttcggctt cgatccgttc
     7261 atcgccagcg ccatggccca ggagatgggg cgctcggaga agcccctgaa ggcggccttc
     7321 tcgaatgccg gcctgcaggc gacctggtgc gcccagggca agcaggcggc cgagttctgg
     7381 ggcaagctct tcaatgtcga ggtgacctgg ttcgacggcg agctcagcgc gccgaagcag
     7441 cgtgccgcca tcgacaacat ggcgacccag gactgggact tcgtcgccat ccaggcgttc
     7501 ggcatcggca cgctgaccga tccggtcaac aagatgatcg atcgcggcat cccggtcatc
     7561 gacatggaca cgctgatcgc cccgctcgag gagatcaacg tccacagctt cctcgccccg
     7621 gacaacgagt tcatgggggc gtcggtgacc caggcgctgg tcgacgcgat cggcggcgag
     7681 ggcaccatgg tgatgaccca gggggcgctg ggccacaccg gggcgcaggg gcgtgcccgc
     7741 ggcttcttct cggtcatcga gaagtatccg ggcatcgagg tgctcgacga gcagcccgcg
     7801 gactgggacg tgaccaaggc gtcgcgcatc tgggacagcc tgctcaccaa gcacccgaac
     7861 atctcggccg ccttcttcca caacgacgac atggcgctgg ccgcctacaa cgtcatgcag
     7921 gcccggggcc ggaccgacat cctgatcggc ggggtggacg ccatgccgcc ggccgtcgac
     7981 gcggtgctgg aagggcacat gtacgccacc gtccgcaacc cgtcctgccg catccacggc
     8041 ggctcgatcg tcgccggcgt ggcggcggtg ctgaccggcg agaagacggg ggacggcatc
     8101 cccaagcaca tcatcaccga cgggccggtg gtgaccaagg ccaacgcgcc cggcatgctc
     8161 tggatgcaga agcactttct catctagggc cgggggtgag cgacggtagc gagcgtccgc
     8221 cgctcatcga gctaagcgga atcggcaaga cgttcggggg cgtcacggcg ctctcgaacg
     8281 tcgatttcgt gttgcgcgag ggcgaggtgc acgggctggt cggcgagaac ggtgccggca
     8341 agtcgacgct gatgaagatc atcgccggcg tgcacaccga gttcgagggc agctaccgcc
     8401 tggacgggca gccggtccgc ctgcgctcga cgcgcgacgc cctggcccgc ggcatcggca
     8461 tggtgcacca ggagctctcg atcgtgccgc atctctcggt tgccgagaac gtctttctcg
     8521 gcaaccagcc ggtgagcagc ctgggtgtcg tgcgctggcg gcacatggcg cgcgaggccg
     8581 aggcgcacct gaagaatctc ggcatcgagg tcgatccgac ggtcgaggcg ggctcgctct
     8641 cgctcggcct gcagcagctg atcgagatcg gccgcgtgct cttctcgggt gcgcggatca
     8701 tcatcctcga cgagcccacc tcggccctct cgccgcccga ggtggagcgg cttttccagg
     8761 tgatcgaccg gctgcggcag ggcggcaaga gcttcatctt catctcgcat ttcctcgacg
     8821 acgtgctcgc catctcggac accgtctcga tcttcaggaa cagccgccac gtgcggacgg
     8881 caccggcagc cgagctcgac aaggggcaga tcatcgagct gatgatcggc cgcggccacg
     8941 aggagctgga ggagagctat ctcggcacgc tggagttgcc gcccgcctcg actgcgccga
     9001 ccctgctcga ggtgcgcggg ctgaccctca agggcgcctt cgaggagctc gatctcgccg
     9061 tccacgaggg cgaggcgctc ggcatctacg gcttcatggg ctgcggccag ctcgagctgg
     9121 tgcgagcgct cgtcggcaag ctcaaggccg atcgtggcac gctcgtcatc ggcggggtcg
     9181 agcggcggct cgccaacacg gcggccggca agcgggccgg ggtggcgctg gtgccggaga
     9241 gccggcgcag catgctgttc gccgactcgc ccatctaccg caacatctcg atcagcgtgc
     9301 tcgagcggat ctcgcgctgg ctcgtcaagc cggtcgccga gcggcagctc gcgcgcgaga
     9361 agatcgagga gctgcggatt cgcgcgggct cggagacggt agcactgcgc acgctctccg
     9421 gcggcaacca gcagaaggtg gcgctcgccc gctggctcgc ctaccggccg aacgtgctgg
     9481 tgctggccga gccgacgcgc ggcatggatg tcggggcgaa ggacgacgtg gtgaagatca
     9541 tccgtgccct gaaggagcag aagatgggcg tgatcgtggt ctcgaccgag cccgagacgg
     9601 tgctgtcgct cgccgatcgc atcctggtca tgcgacgcgg ccggttcacc cgcgagttcg
     9661 tggccgaggc ggtcggcaag gatcaactcc tggcggccgc ctgagccgtt gcgcatgagg
     9721 aaacgcgatc gatgaaggtc tgggttctgc ggcggctgac ggcgatcgca ccgctgatca
     9781 cgctgctttt tctcgtcgcg atcttcgtct cggcgagccc gtccttcgcc accatcgaca
     9841 acgccgccaa catcctgcgc caggtctcga tcacggcgat catcgcgacc ggcctcacct
     9901 tcgtcatcct gaccgccgag atcgacctgt cggtcgccag cgtcgccaac gcggtcggca
     9961 tcctcgtcgc cttcttcacc ctgcaggcgg cctacgtgaa catcgccaac gtgccgctgc
    10021 cgggcgggtt cgccatcctc ttcgccatca tggcggccct ggcactcggc ggcatcaccg
    10081 ccttcggcgt cgtggtgatc ggcatcccct ccttcatcat gacgctcgcc atgatgcaga
    10141 tcggcgccgg catctcggcc ctgctggtgc gcggccagat cgcctacaac gtgccggacc
    10201 tcgtgaagac gctgggctcc ggcagcatcg ccggcatacc ctggatcgtc atcgtcgcga
    10261 cgctctttct cgcggtcggc cacttcgtgc tgagctacac gcgcttcggc cgctacgtct
    10321 acatggtcgg cggcaaccgc gaggcggccg agtatgccgg ggtcaacgtc aagctgatcg
    10381 tggcggcggt gatgatcatc ggcgccttct gctcgggcgt cgccgggatg ctcggcgtcg
    10441 cctattacgg cagcgcgcag cagaaccagt tcgacgactt cctgctcgat tccatcgccg
    10501 cggtcgtggt cggcggcacc agcctgttcg gcggccgggg tggcatcccc aacaccatca
    10561 tcggcctctt cgtgctgggc gtgctcaaca acggcctcga ccacgtcgac atcgacagct
    10621 tcctgaagat cctgattcgc ggcctgatcc tgctcctggc gctggtgatc aacgtctacg
    10681 cgcagaagat ccgggccgac tgacgccccc tgctactcgt ccggcgcctc ggccgcggcc
    10741 ttcctcgcct cgtccaggag ctggccgcag ggcgcgtcct cggcgagccc gaggtcgaag
    10801 aaggggacga gggcggcgat gggtgccagc agccagccga gcgtgttgct ctcgacctcg
    10861 ccaggggccg gggcgatcgt cggcgagcgc cagtcgccct ccacctgcag cggctggttg
    10921 ccgctgagca ggctcgggtc cttcgggtgc gcctcgatca ggaggtccat ggtctcggct
    10981 tcgagatcga tcgtgccggc ggccgtgagc ttggtgtcgg ccgtgtcgag caggagcggt
    11041 cgcgccgtgg cgatgccccg ggcgacctgg aggttggcga cgccgcagcg gatcggcacg
    11101 cgattcgccg cgtcgtccgg ggtgatgagc gtgatcaggc cctccatcag atcgaggccg
    11161 acggcctcga ccaggaaggc atcgagcgag ccgtcggaca tgaccagccc gacctgcccg
    11221 tcgctgctgc ccagcaggtc ggccagggaa tagccccggc cttcgaggcg gacccggccg
    11281 tccacgcggc cggccgcctc gttcgcctcg ccgatccggg ccagcatgtc gtgcaggctg
    11341 aggttggtga ggcgcagatc gacgcggccg accggcgggc gctgggtggt gtcgaacgag
    11401 aagaagagga ccagccggcc gtcggcgagg ccggtcgtga acgggtcgat ggtgatccag
    11461 ccctcgcggg tgaccagatg gatggcgagg cggtcgatcg ggaagagctc ggagcggacc
    11521 tccgcggcgt ccagctcgat gtcgagatcg atgccgcgcc agatcgccgg gtcgatccgg
    11581 aagtcgggga tgagcctcgg ccgctgatcg agcgtctgcg cctcctgccg ctgctcgggg
    11641 ctcgcggtct cgccggcgcc ggtttgcggc gggaggccca cgatggggaa gaggtcgtcg
    11701 aaatcgaggg agcggctccg gaggtcggcc tcgacggcga gcggctgcgc ggcccggtcg
    11761 atccgcaggc tgccggcgat gtcgctgtcg ccgatcgtgc cgctgatgcc gtccagcgtc
    11821 agcaccgatc cttcccgggt gagctcgccc tcgaggtcgt agggtggcgt gtcgatctgc
    11881 gccaggccca ggctggcgag gatggggccg ggattggggc ccgccgcgcg gaacgtgccg
    11941 tgcagggcgg ttgcggcgcc gaagtgatcg acgctgccgt cgaactgcag ggtcgtccgg
    12001 tcgatggtga gccgagcgtc gaccaccgca ccttcgccct gcagctcgcc ctcgagatcg
    12061 agctcgaatg cggggagggt cgggacggcg aggccgagcg tggcgaggaa atcggcgaag
    12121 gcgtcgccgg cctccagatc gagggagaac gtgcccgccg cgggctgcat ggcggccgtg
    12181 atcgatgtgc tggccgcgtc gagcgtcgcc tgcagcggct cgtcggcgcc gtcgacgagc
    12241 gcctgcctcg acggcccgct gccctcgatc gacagcggcg catcgccgag ccggccggca
    12301 ccggtcaggg tcaagggttc ggtcgcaccc cagttgccct ccagctcgtc gagcacgagc
    12361 gtgacgtctc gctcgccatc gcggtagtcg atctgcgcat cttccacgac gaggtggcgg
    12421 atgagcggtg ggatcggcgg gccgtcggtc gatggcgtcg gccgctccgc cggctgccag
    12481 ttcagcgttc cgtcctcgcc ccgcacgacc tccgcatcga ccccctcgac ctcgacgttc
    12541 ggcaggacga tgtcaccgtg caggagcggc cccagtgcca gctccgccgc cgcccgatcg
    12601 acggtcgcca gctcctcgtc ctcgctgttg gcgatggcga gaggccccag cgtcaggcgc
    12661 agggcgccgt ccttccacgc caggtccggc gcgtccccca gctcgatgtc gtagcccgtg
    12721 gcggcgctgg cccgcgcctc gatcacgccg ttgagccagc cctgccgcag ggccagccag
    12781 gcgccggcca gcaggacggt cggcaggccg acgatcagca gaagagcata caggatccag
    12841 cgcaggatat ttctccggtc gatcgtcctg tcgaggcgac gcggacatgc aacgaaccgg
    12901 cgggcaacgc tatggttgcg tcagacctcg gcccacatcc gtagaagatt gtggaaggtc
    12961 ttggtgatcg tgtcgaactc ggtggtcttg cc
//
LOCUS       DAOTFI010000141        12602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5961_length_12602_cov_7.797481, whole genome shotgun sequence.
ACCESSION   DAOTFI010000141 DAOTFI010000000
VERSION     DAOTFI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5961_length_12602_cov_7.797481"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="P5558_14665"
     CDS             <1..329
                     /locus_tag="P5558_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRY25606.1"
                     /translation="DGLLAALQVLAVLCRSQRPMSEIGRSFAPLPQQLRNVRVASAID
                     LQGPALATVLAANEARLVGRGRLVVRPSGTEPLIRVMVEADDEALLEDVISTVADAIE
                     RVAVTA"
     gene            401..1879
                     /locus_tag="P5558_14670"
     CDS             401..1879
                     /locus_tag="P5558_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25607.1"
                     /translation="MLRFRDLFGLLPLPLLTACAGAAPMATTVAELPAVVEPAGGPAA
                     ASAWPEAASCSTLIEELAGLDPAARRGLDPLTAPIALVGIGPLEPGFERDLPIPFSAS
                     ATAAPDPCLLLIGAPRALPAAVDRVLDQRVVRSTYQDGSRRRRNPDHQALERDLAAAK
                     RAPAEDFDVFRTGDPMLDLIGTLAGGVLGGIGQFGAEREISDLETALAATPAYLEQPT
                     ESAYRYELVELEAERRSSLPAALFDRANGTSIALDLTRAERQLFALANDHHPRDLQPS
                     PVPGAALVTTATLAAWRAGSPTLTTSALVEHIAAAIADGAAARPATLGETLAELRRPP
                     PPGPAARANDHVTAGRAMDPAGIARPLAEGLVEVGGEGGARGFYVTAEHIAAPVSALA
                     QSSLVAVRYPDGMAAYGMVELVDDELGLALIYLPRRGEAPERAVAGSAPPPVAGASTS
                     PGVPWVDDERIVGLFVVDSLTAEPRWIDAAELDRFVGRLRTL"
     gene            complement(1902..2264)
                     /locus_tag="P5558_14675"
     CDS             complement(1902..2264)
                     /locus_tag="P5558_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRY25608.1"
                     /translation="MQLGRDAWLGCVLALLAVGAGAFGAHGAGDERAAGLIETAARYQ
                     MWHALAMILVAIALPAARLPVFCFLGGVLAFSGSLYALALGAPAPVAMLAPLGGLAFM
                     AGWALLALAFWRSRRTDA"
     gene            2363..2947
                     /locus_tag="P5558_14680"
     CDS             2363..2947
                     /locus_tag="P5558_14680"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRY25609.1"
                     /translation="MIIRVGIDEVGRGPLAGPVLAAAVHLPQPIAGIADSKMLQPARR
                     EELALLIRQQASWALGAASVAEIDRLNILAASHLAMRRAMRRLLALLPAEPDEVLVDG
                     HLDPGLGCRTRCIVGGDATVAEIGAASILAKVARDRLLARLGRRHPAYGFERHAGYPT
                     LAHRTALALVGASPHHRRSFAPVRRVLEAALAAQ"
     gene            complement(2941..3471)
                     /locus_tag="P5558_14685"
     CDS             complement(2941..3471)
                     /locus_tag="P5558_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25610.1"
                     /translation="MIHLSPGCDAFLQDQVPNGWQDAAGHARRLATRLQYLPWADRVV
                     LLDDFVWGEARRLLSNEEITAVINRQPYTLATSHAILEYATMCSAVITSILMLLEEGG
                     AAEQPEQALALLLSRSAEHQEAALDWIQEGGFERLQTVMARLPGFAFLYLAVYPNDSA
                     ESFMARDAFWTAMLGY"
     gene            3778..6453
                     /gene="mutS"
                     /locus_tag="P5558_14690"
     CDS             3778..6453
                     /gene="mutS"
                     /locus_tag="P5558_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRY25611.1"
                     /translation="MAARRPQPVETDARPGERASPMIEQYQGLKAEHPGSLLFFRMGD
                     FYELFFADAEAAAPALDIALTKRGQHAGRDIPMCGVPVHSAESYLERLIRKGFKVAIC
                     EQVEDPALARQRGARSVVRREVVRIVTPGTLTEESLLDARRHNFLCALARVGGALGFA
                     WVDISTGAFRTEATSADDLGPLLARLAPGEILLARPLAEDAALRAALQPWETRLTLLE
                     PPAFDSQSAALRLQQQFEVATLDAFGRFERAEIAAAGAILDYLATTQKGLLPRLDRPE
                     RQESAACLRIDPATRRNLELLETQDGRRQGALLATIDRTRTGAGARLLAERLAAPLAM
                     LAPIQARLAEVAGFVADEDVREHCRTVLKGVPDIARALSRLVVGRGGPRDLVALATGL
                     AAAHGLRDRLLPAEPALAALADGIADTEALVARLEQLLEPAPPLQARDGGFIRADAVA
                     ELDELRTLRDQGRRHIAALEARYRETTGIATLKIRHNNMLGYFIEVTATHAPKVPDDF
                     RRRQGMVNAQRFGTDELADLERRIASAADRALALELELFEELRGLVLEAAEPIARTAG
                     ALAAIDVAAALAALAIEADWTAPVLTDGLDFSIEGGRHPVVEAALEAGRGRFVANDCR
                     LEDAERLWLLTGPNMAGKSTFLRQNALIVVLAQMGSFVPARSARLGVVDKLFSRVGAA
                     DDLARGQSTFMVEMVETATILNQAGERSFVILDEIGRGTSTYDGLSLAWAVVEHLHEA
                     NRCRGLFATHYHELTALAARLAALGAYTVRVKEWQGDVVFLHEVMAGTADRSYGLHVA
                     RLAGLPHAVLARAQEVLEKLEAGEARAAPAEIAEDLPLFRASLRNRPAAQAPAAIPPH
                     LFALADRLAAARPDDLSPRAALELLYELKALTRDS"
     gene            6422..6949
                     /locus_tag="P5558_14695"
     CDS             6422..6949
                     /locus_tag="P5558_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25612.1"
                     /translation="MSSKPSPGIPEALAALALCLLASGGAALAAGEGFDRAVRGLGRE
                     CVNAASTACAASTFQLADTNGDGRADLAELSTLNDRLRVWTRANAESVPPADLEALNL
                     GFVLIDSIGLQNWIALYDTDGDGALTLAEATADLRLDRRPVPELVRSRELVDWPRLRQ
                     RFGAMSYIFDYLGIR"
     gene            complement(7457..8452)
                     /locus_tag="P5558_14700"
     CDS             complement(7457..8452)
                     /locus_tag="P5558_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRY25613.1"
                     /translation="MHRMFRAPPPHRLAFDAAARRRHKRRNVLHSVLLLVGMAAILWV
                     SAWSLWGTAVAFWAMVGGVLGFVLTPGVAPDWVMRAYRARPLDRRSFPEGVALLEELA
                     ARAGLDHVPRLHLLPSATLNAFAVGSSREAAIGITDGLLRALDARELAAVLAHELSHV
                     RNNDLWLMALADTMSRLTSLMATMGLFLAVLSLPLLLLGMAPVPLLTLAVLILAPTIG
                     SLLQLALSRAREFDADLKAATLTRDPAGLASALRKLDRYQGAFWEEIVLPGRRMPEPS
                     LLRTHPPTAERIERLLSLYGAATLPVSAIGPLRHHLPAGPAYGGPPRYRWPGFWY"
     gene            8550..9452
                     /locus_tag="P5558_14705"
     CDS             8550..9452
                     /locus_tag="P5558_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HRY25614.1"
                     /translation="MAEPSIVGVDWGTSSLRACLLGPDSAVLAERQSEDGILKVEGGA
                     FAPVLRRELAALEAPAGLPIVLSGMITSRQGWHECPYLACPADAKALANALHRVTEPG
                     LGPLHFVTGLEYASPDGLPDVMRGEETQIIGQDDLAPGEIVVLPGTHSKWVAVEEGRI
                     MRFRTWMTGEVFAVLKGHSILGRLMRDAPADAAGFARGVAAGLEGPGLLGRLFSARTL
                     PLTGRLAETAVADYLSGLLIGTEIAEAVAAERPSGVVVVGSDTLTGRYGKALDIAGLR
                     HRPGRSQAAARGQWRIARAAGLVR"
     gene            9449..10093
                     /locus_tag="P5558_14710"
     CDS             9449..10093
                     /locus_tag="P5558_14710"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HRY25615.1"
                     /translation="MSNTALERLDTALATLPLVAILRGLRPDDAEAVGAALVEAGFRL
                     IEVPLNSPDPFKSIEKLARRFGDTAVIGAGTVLETAAVGRLRDAGGELVVMPHADVEV
                     IAEAKAQGLVCVPGIATPTEAFAALAAGADGLKIFPAEILPPKAVKAMLAVLPKGTRL
                     LPVGGIEPATMAGYVAAGAAGFGLGSALFKPEFEPAEIGSRASAFVDAWGELRR"
     gene            complement(10083..10382)
                     /locus_tag="P5558_14715"
     CDS             complement(10083..10382)
                     /locus_tag="P5558_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25616.1"
                     /translation="MLQVRPVFVREAALLLAELAGQATAGDGFARALEERRGGAGRAM
                     ERRPQPERASGFEVHAFLPEGPERAGPPFAFAAYAARLAHRMVFEGPVAGLRLSA"
     gene            complement(10484..11761)
                     /locus_tag="P5558_14720"
     CDS             complement(10484..11761)
                     /locus_tag="P5558_14720"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY25617.1"
                     /translation="MKRRSRGMSDTPRLDAADIGRPFPLLRVFLLILLPLLLVSSVGL
                     VIMLQGAISTSAEKLFADQEIRLVRSMLVADGQFDGRSAPSASIVADLRTEMAEAGIT
                     CLSLVTPDGEPVAEIAGEASCATPDAAAFREVTEDGGGAVLAENIVLPDHWTSLGTVT
                     LPESDEGRAGNDLVVAVTRRSVSLEAAIARLSGFWTIVFGGLFLLAVLATGFVIARAQ
                     GTIDRQAAYVLGVHRRLGRFLSVAAVRTAIDDDAAPTRFSALVMFLDLRDFSSFAEAA
                     PPREVAALVDAFVTAVAGAVADQGGEIDKIVGDGILAFFRGDDATGRALVAAIASIEA
                     CQSLARRPGIGLYRGEVIATALGTGQRADFTILGRTVNLASRLCSLAAENEITMPEDL
                     AAEAGPKLVETSRRLVQPRHHSQELLVVSFRLA"
     gene            complement(11758..12498)
                     /locus_tag="P5558_14725"
     CDS             complement(11758..12498)
                     /locus_tag="P5558_14725"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25618.1"
                     /translation="MASKVAKVTFAFWVMKIIATTLGETTGDFIAQTLALGYVAGLAI
                     TATALIVILVMQIRAERFHAALFWAAIVATTTAGTEVSDLMDRTLGFGYVIGSLVLLA
                     GLAATLLIWRLREKSLSVYPIVDRRVEIMFWIAVVFSNSLGTAFGDALVDVVGLSYIQ
                     GAVVTSAVIGVVLLLHYATSVDKIMLFWIAFVFTRPFGATFGDFLTKPMAKGGLELGT
                     LHASAVALVLLSAIVLVTMWRQRPRAIA"
BASE COUNT         1878 a         4571 c         4396 g         1757 t
ORIGIN      
        1 gcgacgggct gctcgccgcc ctgcaggtgc tggcggtgct ctgccggagc cagcgcccga
       61 tgagcgagat cggccgctcc ttcgcgccct tgccgcagca attgcgcaat gtccgggtcg
      121 ccagcgccat cgacctccag gggccggcgc tggccacggt gctggcggcc aacgaggcgc
      181 ggctggttgg ccgcggccgc ctcgtggtcc ggccgtccgg caccgagcct ttgatccgcg
      241 tcatggtcga ggccgacgac gaggcgctgc tcgaggacgt gatctcgacc gtcgccgacg
      301 cgatcgagcg cgtggcggtc acggcctgag cgccttcggg ttgtaattca accaaaagtt
      361 aatttatgct cttgggcatt ctcgcttgcg aggttgcccg atgctgcgtt tccgcgatct
      421 gttcggcctc ctgccgttgc cgctgctgac cgcctgcgcc ggtgccgcgc cgatggccac
      481 gaccgtggcg gagctgccgg cggtggtcga gcccgccggc ggcccagccg ccgcctcggc
      541 gtggcccgag gcggcttcct gcagcacctt gatcgaggag ctcgccgggc tcgacccggc
      601 ggcgcggcgc gggctcgatc cgctgacggc gccgatcgcc ctggtcggca tcgggccgct
      661 cgagcccggc ttcgagcgcg acctgccgat tccgttctcg gcctccgcaa ccgccgcacc
      721 cgacccctgc ctcctcctca tcggcgcgcc gcgggcgctg ccggccgccg tcgaccgggt
      781 gctcgaccag cgcgtggtcc gctcgaccta ccaggacggc agccggcgcc ggcgcaatcc
      841 cgaccaccag gcgctggagc gcgacctcgc cgcggccaag cgggcgccgg ctgaggattt
      901 cgacgtcttc agaacgggcg atccgatgct cgatctaatc ggcaccctgg ccggcggcgt
      961 gctcggcggc atcgggcagt tcggggccga gcgcgagatc agcgacctcg agaccgcgct
     1021 cgcggcgacg ccggcctatc tcgagcagcc gaccgagtcc gcctatcgct acgagctcgt
     1081 cgagctcgag gcggagcgtc gctcgagcct gccggccgcg ctcttcgacc gggcgaacgg
     1141 gacgagcatc gccctcgatc tgacccgcgc cgagcgccag ctcttcgcgc tcgccaacga
     1201 ccatcatccc agggatctgc agccctcgcc cgtgccaggt gctgcactgg tgacgaccgc
     1261 gacgctcgcc gcctggcggg ccggcagccc caccctgacg acgagtgcgc tcgtcgagca
     1321 catcgccgcc gccatcgccg atggtgcggc agcccgccct gccacgctcg gtgagacgct
     1381 ggccgagctg cgccggccgc cgccgcccgg gccggccgca cgggcgaacg atcacgtcac
     1441 cgccgggcgg gcgatggacc cggccggcat cgcccgcccg ctggccgagg gcctggtcga
     1501 ggtcggcggc gagggtggcg ccaggggctt ctacgtgacg gccgagcata tcgccgcacc
     1561 ggtgagcgct ctcgcccagt cgagcctggt ggccgtccgc tacccggacg gcatggccgc
     1621 ctatggcatg gtcgagctgg tcgacgacga gcttggcctg gcgctgatct acctgccccg
     1681 ccgtggcgag gcgcccgagc gggccgtcgc cggctcggcg ccgccgccgg tcgccggggc
     1741 gagcacgtcg cccggcgtgc cctgggtgga cgacgagcgg atcgtcgggc tcttcgtcgt
     1801 cgacagcctg accgccgagc cgcgctggat cgatgcggcg gagctcgacc gcttcgtcgg
     1861 ccggctccgg acgctctgac cggtcgccgc ccgccgaatt gtcaggcatc cgtgcggcgg
     1921 ctccgccaga aggcgagggc gaggagcgcc cagccggcca tgaaggcgag gccgccgagc
     1981 ggtgcgagca tcgccaccgg ggcgggtgct cccagcgcca gcgcatagag gctcccggag
     2041 aaggcgagga cgccacccaa aaagcaaaaa accggcaggc gggccgccgg cagggcgatc
     2101 gccacgagga tcatggccag cgcgtgccac atctggtagc gcgccgccgt ctcgatcagc
     2161 ccggccgccc gctcgtcgcc ggcgccatgc gcgccgaagg cgccggcccc gaccgcgagc
     2221 agcgccagga cgcagccgag ccaggcatcc ctgccgagtt gcatccgccg ccttcgcgta
     2281 gtgggttggc cgtgccttcg agatatgcga tttccggggc tgcgcaacag gtgcccgggt
     2341 gtcgcggcag ggcggaacgg cgatgatcat cagggtcggc atcgacgaag tgggtcgcgg
     2401 gccgctcgcc ggcccggtgc tggccgccgc cgtccacctg ccgcaaccca tcgcgggtat
     2461 cgccgattcg aagatgttgc agccggcacg gcgggaggag ctggcgctgc tgatccgcca
     2521 gcaggcgagc tgggcgctcg gtgcggcctc ggtcgccgag atcgaccggc tcaacatcct
     2581 cgccgccagc catctcgcca tgcggcgggc gatgcggcgc ctcctggcgc tcttgcccgc
     2641 cgaaccggac gaggtgctgg tcgacggcca tctcgacccg gggctcggct gccgcacgcg
     2701 ctgcatcgtc ggcggcgacg ccacggtggc cgagatcggc gccgcctcga tcctcgccaa
     2761 ggtcgcccgc gaccggctgc tggcccgtct cggccggcgc cacccggcct acggcttcga
     2821 gcgccacgcc ggctacccga cgctggcgca ccgcacggcg ctggccctgg tcggggcctc
     2881 gccgcatcat cgccggagct tcgccccggt gcgccgcgtg ctcgaagcgg ctctcgccgc
     2941 tcagtagccg agcatcgccg tccagaaggc gtcgcgcgcc atgaagctct cggccgaatc
     3001 gttgggatag acggcgagat agaggaaggc gaagccgggc agccgggcca tcacggtttg
     3061 cagccgctcg aacccgccct cctggatcca gtcgagcgcc gcctcctgat gctcggcgct
     3121 gcgcgacagc agcagggcga gcgcctgctc cggctgctcg gccgcgccac cctcctcgag
     3181 cagcatgagg atgctggtga tcacggcact gcacatcgtc gcgtattcga ggatcgcgtg
     3241 gctggtggcc agcgtgtagg gctgccggtt gatgaccgcc gtgatctcct cgttggagag
     3301 aagccggcgg gcctcgcccc agacgaaatc gtcgagcagc accacccggt cggcccaggg
     3361 caggtattgc agccgcgtcg cgaggcgccg ggcgtgcccg gccgcgtcct gccagccgtt
     3421 cggcacctgg tcctgcagga aggcgtcaca gccgggcgag agatggatca tgcgaccctc
     3481 ccggggcggc gacgacccgc ctcgccacgt tggccagccc ggcgccggcc gagcgcttcc
     3541 tcgtgccggg cagccgcatg gctgcccgct cgttccttcg acatcctcgc cctctcctcg
     3601 ctcggcgcgc tcggcactgc ccatgcgttg ctcgctcgac cgacgacgct tcctcggcga
     3661 tagtgacctt gttttcttgg cccccggccg ggcgtcgatc atcaccggtc gtcccgagag
     3721 cgcatgatag gaaatgacgc gagcgtggca acccaaaatt caactatagg ttactagatg
     3781 gcggcgcgac gtccccagcc ggtagaaacc gatgcgcgac ccggcgagcg tgccagcccg
     3841 atgatcgagc aatatcaggg gctcaaggcg gagcacccgg gatcgctgct gttcttccgc
     3901 atgggcgatt tctacgagct cttcttcgcc gacgccgagg cggccgcacc ggcgctcgac
     3961 atcgcgctca ccaaacgcgg ccagcacgcg ggccgggaca ttccgatgtg cggcgtgccg
     4021 gtgcatagcg cggagagcta tctcgagcgg ctgatccgca agggcttcaa ggtggcgatc
     4081 tgcgagcagg tcgaggatcc cgccctcgcc cgccagcgcg gcgccaggag cgtcgttcgc
     4141 cgcgaggtcg tgcgcatcgt cacgccgggc acgctcaccg aggagagcct cctcgatgcg
     4201 agacgccaca acttcctctg cgccctggcc cgggtgggcg gtgccctggg ctttgcctgg
     4261 gtcgacatct cgaccggcgc ctttcggacc gaggccacct cggccgacga tctcgggccg
     4321 ctgctcgccc gcctcgcccc cggcgagatc ctgctggcgc ggccgctcgc cgaggacgcc
     4381 gccctccggg cggcactgca gccctgggag acgcgcctga ccctcctgga gccacccgca
     4441 ttcgacagcc agtcggccgc gctgcggctg cagcagcagt tcgaggtcgc cacgctcgat
     4501 gcgttcgggc gcttcgagcg ggccgagatc gccgccgccg gcgccatcct cgactatctc
     4561 gcgaccaccc agaagggcct gctgccgcgc ctcgaccggc cggagcggca ggaatccgcc
     4621 gcctgcctgc gcatcgaccc ggcgacccgc cgcaatctcg agctcctgga aacccaggac
     4681 ggccgccgcc agggtgccct gctcgccacc atcgaccgga cccgcaccgg cgccggcgcc
     4741 cgcctcctgg ccgaacggct cgccgccccg ctcgccatgc tcgccccgat ccaggcgcgg
     4801 ctcgccgagg tcgcgggctt cgtcgccgac gaggacgtcc gcgagcactg ccgcaccgtg
     4861 ctcaagggcg tgcccgatat cgcccgggcc ctttcccgcc tcgtggtcgg ccgcggcggc
     4921 ccgcgcgatc tcgtggccct cgccacgggc ctcgcggccg cgcacggcct gcgggaccgg
     4981 ctccttccgg cggagccggc gctcgccgcc ctggccgatg gcatcgccga cacagaggcc
     5041 ctcgtcgccc gcctcgagca gctgctcgag ccggcgccgc cgctgcaggc ccgggacggc
     5101 ggcttcatcc gcgctgatgc ggtggccgag ctggacgagc tgcgcacact ccgggatcag
     5161 ggccgccgcc acatcgccgc cctcgaggcg cgctaccgcg agacgaccgg catcgccacg
     5221 ctgaagatcc gccacaacaa catgctgggc tacttcatcg aggtcacggc gacccatgcg
     5281 ccgaaggtgc cggacgattt ccgccggcgg cagggcatgg tcaacgccca gcgcttcggc
     5341 accgacgagc tggccgatct cgagcggcgc atcgccagcg ccgccgaccg ggcgctggcg
     5401 ctcgagctcg agctcttcga ggagctgcgc ggcctggtgc tggaagccgc cgagcccatc
     5461 gcgcggacgg ccggggcgct ggcggcgatc gacgtcgcgg cagcgctcgc cgccctcgcc
     5521 atcgaggccg actggacggc acccgtcctc accgacggcc tcgacttcag catcgagggc
     5581 ggccggcacc cggtggtcga ggcggcgctc gaagccggcc ggggacggtt cgtcgccaat
     5641 gattgtcggc tggaggatgc ggagcggttg tggctgctca ccggccccaa catggccggc
     5701 aagagcacct tcctgcgcca gaacgcgctc atcgtcgtgc tcgcccagat gggcagcttc
     5761 gtgccggccc ggagcgcgag gctcggtgtg gtcgacaagc tgttcagccg ggtcggcgcc
     5821 gccgacgacc tggcacgcgg ccagtcgacc ttcatggtcg agatggtcga gacggcgacc
     5881 atcctgaacc aggccggcga gcgcagcttc gtcatcctcg acgagatcgg ccggggtacc
     5941 tcgacctatg acggcctgtc gctcgcctgg gcggtggtcg agcacctgca cgaagccaat
     6001 cgctgccgcg gcctcttcgc cacccactat cacgagctca ccgccctggc ggcccggctc
     6061 gccgcactcg gcgcctacac cgtccgggtg aaggagtggc agggcgacgt cgtcttcctg
     6121 cacgaggtga tggccggcac cgccgaccgc tcctatggtc tgcacgtcgc ccgcctcgcc
     6181 ggcctgccgc atgccgtgct ggcccgggca caggaggtgc tggaaaagct cgaggccggc
     6241 gaggcccggg cggcaccggc cgagatcgcc gaggatttgc cgctctttcg cgccagcctg
     6301 aggaaccggc cggccgcaca ggcaccggcg gccattccgc cccacctctt cgcccttgcc
     6361 gaccgtctcg ccgccgcgcg gccggacgac ctctcaccgc gcgccgccct ggagttgctc
     6421 tatgagctca aagccctcac ccgggattcc tgaagccctc gccgccctcg cactctgcct
     6481 gctcgccagc ggcggcgcgg cgctcgccgc cggcgaaggc ttcgatcggg cggtgcgcgg
     6541 gctcggccgg gaatgcgtca acgccgcctc gacggcctgt gccgcgagca ccttccagct
     6601 cgccgacacc aatggcgacg gccgggccga tctcgccgag ctttcgaccc tgaacgaccg
     6661 cctgcgcgtc tggaccaggg ccaacgccga gagcgtgccg ccggccgacc tcgaggccct
     6721 caatctgggc ttcgtgctca tcgacagcat cggcctgcag aactggatcg ccctctacga
     6781 cacggacggc gacggtgccc tgaccctcgc cgaggcgacc gccgacctga ggctggaccg
     6841 gcgaccggtg cccgagctcg tgcgcagccg cgagcttgtc gactggccaa ggctgcgcca
     6901 gcgcttcggc gccatgtcct atatcttcga ctatctcggg atcaggtgac ccgctcagcg
     6961 aaagatggcc acgagacgcc acggcgccgc gccgctgtcc tggccgaccg gccataacga
     7021 ttcgttatgg cctctttcgg cctgacggca ttccgcggag cctttgtgcc gtatactgcg
     7081 acatcggcct cgggttggtg cgtctgttgc aacaaagtgg catggcctgt cgcaccggac
     7141 gacacagaac agcggcaaat gtagcgccag atgcgacata acgatcgctt atggcgcatc
     7201 ttctgcgaca ttggcgacga acgaacctgc ggaatcaacg agcaaacccg gggaatcgac
     7261 gatcgaaccc agggaatcga cgaacgaacc cgacccgcga ccaacgaacc cgatcgccca
     7321 taactcattg aatcgtaaca aatcagttcc gcccgagagg gttgtttcgc agtcgccggg
     7381 ccttcagacg ccggaaatgc cgaacgaacc gacgccgact tgaccgtcgg gccgatctct
     7441 gatattattc ctatactcag taccagaagc ccggccagcg atagcggggc gggccaccat
     7501 aggcgggtcc ggcgggcagg tggtgccgca gcgggccgat cgccgacacc ggcagggtgg
     7561 cagcaccata gagcgagagc aggcgctcga tgcgctccgc cgtcggcggg tgggtgcgca
     7621 ggagcgaggg ctccggcatg cgccggccgg gcagcacgat ctcctcccaa aaggcgccct
     7681 ggtagcggtc gagcttcctg agcgccgagg ccagcccggc ggggtcccgg gtgagggtcg
     7741 cggccttgag gtcggcgtcg aattcccgcg cccgcgacag ggcgagctgc aggaggctgc
     7801 cgatggtcgg cgccaggatg agaacggcaa gcgtcagcag cggaaccggc gccatgccga
     7861 gcagcagcaa aggcaggctg agcacggcga gaaagaggcc catggtcgcc atgaggctgg
     7921 tcagccggct catcgtgtcg gcgagcgcca tgagccagag gtcgttgttc cggacatggc
     7981 tgagctcgtg agccaggacg gcggcgagct cgcgggcgtc gagggcacgc aggagaccgt
     8041 ccgtgatccc gatcgctgcc tcgcggctgc tgccgacggc aaacgcgttg agcgtggcgg
     8101 agggcaggag atggagcctc ggcacatggt cgaggccggc acgggccgcg agctcctcga
     8161 gcagggcaac gccttccgga aagctccgcc ggtcgagcgg ccgtgcccgg taggcccgca
     8221 tcacccagtc cggggcgacg ccgggggtga ggacaaagcc gaggaccccg cccaccatcg
     8281 cccagaaggc cacggccgtg ccccagaggc tccaggccga gacccagagg atggccgcca
     8341 tgccgacgag cagcagcacc gaatgcagca cgttgcgccg cttgtgccgg cgccgggcgg
     8401 cggcatcgaa ggcgaggcgg tggggcggcg gtgctcggaa catcctgtgc atggccgaca
     8461 tctaggcaga accgggccgc cggccaacgg cggattgatc ccggctcctc gacctgcgac
     8521 aagtcgggcc ggaagccggg aggaaagcgg tggcggagcc ttcgatcgtc ggggtggact
     8581 gggggacctc gagcctgcgc gcttgcctgc tcggcccgga tagcgccgtg ctggccgagc
     8641 ggcagtccga ggacggcatc ctgaaggtcg agggcggggc ctttgcaccc gtgctccggc
     8701 gcgagctcgc ggcgctcgag gcacctgccg gcctgcccat cgtcctctca ggcatgatca
     8761 ccagccgcca gggctggcac gaatgcccct atctcgcctg cccggccgat gccaaggccc
     8821 tcgccaacgc cctgcatcgc gtcaccgagc ccgggctcgg ccccctccat ttcgtcaccg
     8881 gcctcgaata cgcgagcccc gacggcctgc ccgacgtgat gcgcggcgag gagacccaga
     8941 tcatcggcca ggacgacctc gccccgggcg agatcgtcgt cctgcccggc acgcacagca
     9001 agtgggtcgc cgtcgaggag ggccgcatca tgcgctttcg cacctggatg accggcgagg
     9061 tcttcgccgt gctcaagggc cactcgatcc tcggccggct gatgcgcgac gcgcccgcag
     9121 atgcggccgg cttcgcgcgc ggcgtcgctg ccgggctcga ggggccgggc ctgctcggcc
     9181 ggctcttcag cgcccgcacc ctgccgctca ccggccgcct cgccgaaacg gccgtggccg
     9241 actatctctc cggcctgctg atcggcaccg agatcgccga ggcggtggcc gccgagcggc
     9301 cctcgggcgt cgtcgtggtc ggcagcgaca ccctgaccgg gcgctatggc aaggcgctcg
     9361 acatcgccgg tcttcgccat cgtcccggcc ggtcgcaggc ggcggcccgc ggccagtggc
     9421 gcatcgcccg ggccgcgggg ctcgtccgat gagcaacacc gcgctcgagc gtctcgacac
     9481 cgccctggcg acattgccgc tcgtggccat cctgcgcggg ctccggcccg acgacgccga
     9541 ggcggtcggc gccgccctgg tcgaggccgg gttccggctg atcgaggtgc cgctcaactc
     9601 gccggacccc ttcaaaagca tcgagaagct cgcccgccgc tttggcgaca ccgccgtcat
     9661 cggcgccggc acggtgctcg agaccgccgc cgtcggccgc cttcgggatg ccggcggcga
     9721 gctcgtcgtc atgccgcatg ccgacgtcga ggtcatcgcc gaggccaagg cgcagggcct
     9781 cgtctgcgtg cccggcatcg ccaccccgac cgaagccttc gcggccctcg ccgccggggc
     9841 cgacgggctc aagatcttcc cggccgagat cctgccaccc aaggcggtca aggcgatgct
     9901 ggcggtgctg ccgaagggca cccgcctgct gccggtcggc ggcatcgagc ccgccaccat
     9961 ggcaggctat gtcgccgccg gcgctgcggg cttcggcctc ggctcggcgc tcttcaagcc
    10021 ggagttcgaa ccggccgaga tcggctctcg cgccagcgcc ttcgtcgatg cctggggcga
    10081 gctcaggcgc tgagccggag ccctgccacc ggcccctcga agaccatgcg atgggcgagg
    10141 cgcgcggcat aggcggcgaa ggcgaagggc gggcctgcgc gttccggccc ctccggcagg
    10201 aaggcatgca cctcgaaccc cgaggcgcgt tccggctgcg gacgccgctc catcgcccgc
    10261 ccggccccgc cccggcgctc ctccagcgcc cgcgcgaacc cgtcacccgc cgtcgcctgg
    10321 ccggcgagct cggccagcag cagggcggct tcgcgcacga agacggggcg gacctggagc
    10381 atggttcggt tatcgcgcgt tagggttaac aaccggtgaa ggagaggcgg catgcgagat
    10441 catgttcgcg ggcgtggtgt agccgacggt cgccttcggc ggatcatgcc agcctgaacg
    10501 agacgacgag gagttcctgg ctgtgatgtc ggggctgcac cagccttctg gaagtttcca
    10561 cgagcttcgg gcctgcctcg gcggcgaggt cctcgggcat cgtgatctcg ttttccgccg
    10621 ccagcgagca gaggcgcgag gccaggttca cggttcggcc gaggatggtg aaatcggccc
    10681 tctgaccggt gccgagcgcc gtggcgatga cctcgccccg gtagaggccg atcccgggac
    10741 ggcgggccag cgactggcag gcctcgatgc tggctatggc ggcgacgagg gcgcgcccgg
    10801 tcgcgtcgtc gccgcggaag aaggcgagga tgccgtcgcc gacgatcttg tcgatctcgc
    10861 cgccctggtc ggcgacggca ccggcgaccg ccgtgacgaa ggcgtcgacg agggcagcga
    10921 cctcgcgcgg cggtgccgcc tcggcgaagc tcgagaagtc gcgaagatcg aggaacatga
    10981 ccaaggcgga aaaacgtgtt ggcgccgcgt cgtcgtcgat cgccgtgcgg acggctgcga
    11041 ccgaaaggaa acggccgagc cgccggtgga caccgagcac gtaggccgcc tggcgatcga
    11101 tcgtcccctg cgcccgggcg atgacgaacc ctgtcgccag tacggcgaga aggaagagcc
    11161 caccgaacac gatcgtccaa aagccgctca gcctggcgat cgccgcctcc agcgagacgc
    11221 tgcggcgggt cacggccacc acgagatcgt tgccggcccg gccctcatcc gactcgggaa
    11281 gcgtcacggt gcccagcgat gtccaatgat cgggcaggac gatgttttcc gccagcacgg
    11341 cgccgccgcc atcctcggtc acttcacgga aggcggcggc atccggggtc gcgcaggacg
    11401 cctcaccagc gatctcggcg acgggctcgc cgtccggcgt gacgagggaa aggcaggtga
    11461 tccctgcttc cgccatctcg gtccgcaggt cggcgacgat cgacgccgaa ggcgccgacc
    11521 gcccatcgaa ctggccgtcg gcgaccagca tggagcggac gagcctgatc tcctggtcgg
    11581 cgaacagctt ctcggcactc gtggagatgg cgccctgcaa catgatcacg agcccgaccg
    11641 agctgacgag gagcagcggc agcaggatca ggaggaagac gcggaggagc gggaacggcc
    11701 tgccgatgtc ggcggcgtcg agccgcggcg tgtcggacat gccgcgactg cgccgcttca
    11761 cgcgatcgct cgagggcgtt gccgccacat ggtcacgagg acgatggcgg acaggaggac
    11821 gagcgccacg gccgacgcat ggagggtgcc aagctccagg ccgcccttgg ccatgggctt
    11881 ggtgagaaaa tcgccgaagg tcgcgccgaa cggacgcgtg aagacgaagg cgatccagaa
    11941 gagcatgatc ttgtcgacgc tcgtcgcata atgcagaagc agcacgacgc cgatcacggc
    12001 cgaggtcacg actgcaccct gaatgtagct gaggccgacg acgtcgacga gggcatcccc
    12061 gaaagctgtg ccgagactgt tggagaacac caccgcgatc cagaacatga tctcgacacg
    12121 gcgatcgacg atcgggtaga cgctcaggct cttttcccgc aggcgccaga tgagcaaagt
    12181 cgcggccagg ccggcgagca gcaccagcga gccgatgacg tagccgaagc cgagcgtgcg
    12241 atccatgaga tcggacacct cggtgccggc cgtcgtcgtc gcgacgatgg cggcccagaa
    12301 cagggcggca tgaaacctct cggcgcgaat ctgcatcaca aggatcacga tcagcgccgt
    12361 ggcggtgatg gccagcccgg cgacatagcc gagagcgagc gtctgcgcga tgaagtcgcc
    12421 ggtcgtctcg cccagtgtcg tggcgatgat cttcatgacc cagaacgcga atgtgacctt
    12481 cgccaccttg ctggccatgt catggctcgc cgaatcgtgc attcctggcg ccttttctcg
    12541 ggtggggggc gtggattccg tgatgaggtg caacaccgtg atgaatcggg catctatctg
    12601 at
//
LOCUS       DAOTFI010000142        12584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_5975_length_12584_cov_8.002554, whole genome shotgun sequence.
ACCESSION   DAOTFI010000142 DAOTFI010000000
VERSION     DAOTFI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5975_length_12584_cov_8.002554"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1094)
                     /gene="ilvC"
                     /locus_tag="P5558_14730"
     CDS             complement(75..1094)
                     /gene="ilvC"
                     /locus_tag="P5558_14730"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRY25619.1"
                     /translation="MRVYYDTDADLNLVKSRKVAVVGYGSQGFGHANNLKDSGVTDVV
                     VALKEGSASRAKAERAGFRCMTASEAAKWADIVMVLVPDELQADLYKNELRDNMKEGA
                     ALMFAHGFNIHFNLIEPRPDLDVLMIAPKGPGHLVRAEYTKGAGVPCLLAIHQDATGG
                     AHDVGLSYGAAIGGGRAGIIETSFKEEVETDLFGEQVVLCGGLCALIQAGFETLTEAG
                     YAPEMAYFETVHEVKLIVDLIYEGGLANMRYSISNTAEYGDYKTGPRIVTAETKAEMK
                     RILADIQEGRFARDWVLENKADQAHFKAIRRRHAEHPVEEIGTKLRAMMPWIGANKLV
                     AGTKD"
     gene            complement(1149..1715)
                     /gene="ilvN"
                     /locus_tag="P5558_14735"
     CDS             complement(1149..1715)
                     /gene="ilvN"
                     /locus_tag="P5558_14735"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRY25620.1"
                     /translation="MPAKESKPSTPQALFSDAERTPEERHTIVVLVDNEPGVLARVIG
                     LFSGRGYNIESLTVAEIDADKSQSRINIVTTGTPLVIEQIRNLLAKLVPVHSVLDLTV
                     EGPHIERELALVKVVGGRENLVQALRIADFMHARLVDSTPESFVFEVTGTSGEVDAFI
                     HLVEPIEVSRAGVVAIGRGESILRPRER"
     gene            complement(1808..3601)
                     /locus_tag="P5558_14740"
     CDS             complement(1808..3601)
                     /locus_tag="P5558_14740"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HRY25621.1"
                     /translation="MTAATAAPPAVDTQSMTGSQVVLKALVEQGVEVIFGYPGGAVLP
                     IYDAIFQQDKLRHILVRHEQAAVHAAEGYARSTGKVGCVLVTSGPGATNTVTGLTDAL
                     MDSVPIVCLTGQVPTHMIGNDAFQEADTVGITRPCTKHNYLVKDPEDLARSVHEAFHV
                     ARSGRPGPVVVDLPKDILMKPAPYQGKDAARRRTYQPARDPDPRRIEEAVELMTRAER
                     PIFYVGGGVINAGPRACELLAELVRETGYPITLTLMGLGAYPASDRQFIGMLGMHGTY
                     EANMAMHGADVMINIGARFDDRVTGRLDGFSPGSRKIHVDIDPSSINKNVAVDVPVIG
                     DAEPTLEALLAAWRRRSNSLALGRTAAWWEQIADWQARKSLRFRQGTDSIKPQYAIQR
                     LHQLTRDRDVYITTEVGQHQMWAAQHFPFDKPFHWMTSGGLGTMGYGFPAAMGVQVAH
                     PEALVIDIAGESSWLMNMQEMSTCMQYRLPVKSFILNNSYMGMVRQWQDFFHGNRHAE
                     SYMDSLPDFVKLAEAFGAVGLRAEKPSEVDDLIKEMIKVKQPVIADIVVDRTENVYPM
                     IPGGAAHNEIKLSPEDDGAHEAISEKGMVLV"
     gene            complement(3800..4744)
                     /gene="miaA"
                     /locus_tag="P5558_14745"
     CDS             complement(3800..4744)
                     /gene="miaA"
                     /locus_tag="P5558_14745"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRY25622.1"
                     /translation="MRVTGGLVLIGGSTASGKSALALALAERTGGAIVNADSVQLYRD
                     LPILTARPPAAELARAEHHLYGILEAHEASSVAAWLARLRPVLAGLEAQRRPAILTGG
                     TGLYFKALLEGLPAIPEIPPDLRAGLRADPAPAEALHARLARLDPLLAARLEPGDRQR
                     ILRGLEVVLATGRKLTDWQGDPPVRPPLPEPCVGIALLPPADLVLPRIERRLDAMLEQ
                     GVLAELAAFRARTGDRPTMLSGADGVAELGAHLDGRIDLAEARRLTVIKVRRYAKRQR
                     TFFRGQLAGTLEPHPVAVGPDNLDALAAALLEGLRDAT"
     gene            4769..5656
                     /gene="serB"
                     /locus_tag="P5558_14750"
     CDS             4769..5656
                     /gene="serB"
                     /locus_tag="P5558_14750"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRY25623.1"
                     /translation="MPPTGPQCLLLVANPALAPLEPALVETLGRRHGGTVRWLADSAA
                     VELALDPCPPRETLAEALAGRALDWALVPAAGRKKRLLVSDMDSTIIDIECIDELADF
                     VGIKAEVAAITRRAMNGELDFAAALTERVALLEGVEEKAIAEICRERLRLNPGARTLV
                     STMRAAGALTILVSGGFTLFTAFVARLAGFERHHGNRLEIRDGRLTGRVLPPILGALA
                     KLETLDEAAAGLGLDREAALALGDGANDRPMLEAAGLAVAWRPHPVLAAAADLVLAHA
                     DLAAALYLQGYTAAEIAPS"
     gene            complement(5661..7040)
                     /locus_tag="P5558_14755"
     CDS             complement(5661..7040)
                     /locus_tag="P5558_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRY25624.1"
                     /translation="MAQTLPTQGFGDLVERVGPAVVNIQTSREAVMQEDIPLPQFPPG
                     SPFEEFFKDFFDREQQQPQRPRRGASLGSGFVIAPDGFVVTNNHVIAEADEITVAFAD
                     GKEYSAELIGRDQQTDLALLKIERDEPFPFVDWADSDEVRVGDWMLAIGNPFGLGTSV
                     TAGIVSARGRDIRAGPYDDFFQVDAAINRGNSGGPSFTMDGQVFGVNTAIFSPSGGNV
                     GIGFAIPSNLAKRVIDSLKSDGRVARGWLGVRIQSVTDEIAASLGLDEAQGALVASVT
                     PGGPAEDAEIQAGDVILEFNGQEITQMRSLPRIVAETDVGREVDVLIWRRGEERTVRV
                     TLGELPDDATLSELGNGDEPPASTALAIDELGLTVSALDDEMRTRFELGQDVRGVVII
                     DVEPGSAADEEALRPGDLIVEVSQSEVGSPPEVLAKVNEAIDEGRDAVLLLIDRQGDF
                     RFVALRLAA"
     gene            complement(7332..7517)
                     /locus_tag="P5558_14760"
     CDS             complement(7332..7517)
                     /locus_tag="P5558_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HRY25625.1"
                     /translation="MQDLLTASALVLVLEGALWALFPDAMKRAAARAMTLESGMLRAA
                     GLAFAVLGVFIVWLIRR"
     gene            complement(7607..8620)
                     /locus_tag="P5558_14765"
     CDS             complement(7607..8620)
                     /locus_tag="P5558_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRY25626.1"
                     /translation="MSHRLLIGLAVAVVAGFFILSSALFTVHQTQQALVLQFGNPVRV
                     VTEPGLNIKVPFIQQVEYFERRVLDFDAGSVELVLGDQKRLVVDAFTRYRITDPLRFR
                     QSVGSEAAFRGRLEPITFSALRSVLGEVSLFTILSDERTRLMNRIRDEANASLQQFGV
                     QVVDVRIKRADLPAENSQAIFLRMQTEREREARELRAQGAEIAQRIRARADRERRVLI
                     AEAGREAEQIRGRGDSVAIGTFAEAFGRDADFFAFYRSMQAYRNALAGDATSYVLQPD
                     SEFFRYFNSFEGLPAEEVAGAPTAPPAVADAAAGAPAEAATDASAVPPVAELPATAPQ
                     SAQ"
     gene            complement(8617..9813)
                     /gene="hflK"
                     /locus_tag="P5558_14770"
     CDS             complement(8617..9813)
                     /gene="hflK"
                     /locus_tag="P5558_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRY25627.1"
                     /translation="MPWNSQGGGGWQGGGGQGPWGNRPGGGGGGPQPPDLEELIRKSQ
                     EKVKSLFPGGGGGGGRGLSAKAISIVVLALLGIWLASGFYRVQPEEEGVVLRFGKLNR
                     VTTPGLNYHLPSPIESVLTPQVTRVNRVEVGFRSGTGASGSGRGITEEALMLTGDENI
                     IDVNFVVLWRISNAADYLFNIRDPEDTVSIAAESVMREVIGQTPILEATTEGRRAIEG
                     RVREQMQVLMNEYGAGINIEEVQLQKSDPPAEVIDAFRDVQRAQADRERLQNEAEAFA
                     NDIIPRARGEAERLLQEAQAYEQEAVERARGEADRFTQVLEAYGKAKDVTIKRIYLET
                     MEEVLGGVNKVIIDSQGGGNGVVPYLPLPDLERRARERDAAASAAAGSAPVERGFGAM
                     QSGATQ"
     gene            9944..11047
                     /gene="apbC"
                     /locus_tag="P5558_14775"
     CDS             9944..11047
                     /gene="apbC"
                     /locus_tag="P5558_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HRY25628.1"
                     /translation="MDELSPDRIRQALADVRHPTSGQSVVTLGMVSGIVVKGRNVGFA
                     LEVPAADAQKLEPLRRACEEAVKALPGVGSCTVVLTAERGPPPDMQARPAAPAPTRAG
                     PPGIDKIIAVASGKGGVGKSTTAINLACGLRAAGLRVGLLDADIYGPSMPRMAGLSGR
                     PTTADGKKMQPMEVAGVKLMSMGFLVEEETPMIWRGPMVQSALQQMLGDVAWGELDIM
                     VVDMPPGTGDAQLTMAQKVPLAGAIIVSTPQDIALLDARKAINMFRRVDVPILGIVEN
                     MSYFCCPNCGHRTEIFAHGGARECAAKYNVDFLAEIPLDIVIRETSDSGRPIVVSQPD
                     SPQAQAYMNLALTVRDKLAGPTGVKPFPRIVDA"
     gene            complement(11064..11492)
                     /gene="atpC"
                     /locus_tag="P5558_14780"
     CDS             complement(11064..11492)
                     /gene="atpC"
                     /locus_tag="P5558_14780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRY25629.1"
                     /translation="MAEASTDKLAFELVSPERVLASVHADMVVVPGEDGDFGVLVEHA
                     PLLSLLRPGVISIYQGQQVEQRIFVEGGFAEVNPKGLIVLAEAAAPMGELNLDDARQS
                     LKDAEEDLGDARDPTEPERERLERAITVARARVEALEFQG"
     gene            complement(11509..>12584)
                     /gene="atpD"
                     /locus_tag="P5558_14785"
     CDS             complement(11509..>12584)
                     /gene="atpD"
                     /locus_tag="P5558_14785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRY25630.1"
                     /translation="FVDQSTEAEILVTGIKVVDLLTPYPKGGKIGLFGGAGVGKTVLI
                     MELINNIAKAHGGVSVFAGVGERTREGNDLYHEMVESGVIVEDDRSKSKVALVYGQMN
                     EPPGARARVGLTGLTLAEYFRDQEGQDVLFFVDNIFRFTQAGSEVSALLGRIPSAVGY
                     QPTLGTDMGGLQERITSTNKGSITSVQAIYVPADDLTDPAPATAFSHLDATTVLSRSI
                     AELGIYPAVDPLDSTSRILEPGVVGEEHYRVARQVQETLQRYKALQDIIAILGMDELS
                     EEDKLVVARARKIQRFLSQPFFVAEVFTGSPGKLVPVEETIKGFKGIVAGDYDHMPES
                     AFYMVGSIDEAIEKAKKLAAEAA"
BASE COUNT         1790 a         4272 c         4540 g         1982 t
ORIGIN      
        1 tgtctccgta cagctgttaa tgatgtctcc ggtccataca gagggtcacc ctcccctggc
       61 ttctttcccg tccgtcagtc cttggtcccc gcgaccagct tgttggcgcc gatccagggc
      121 atcatggcgc ggagcttcgt gccgatctcc tcgacggggt gctcggcgtg gcggcggcgg
      181 atggccttga agtgggcctg gtcggccttg ttctcgagga cccagtcgcg ggcgaagcgg
      241 ccttcctgga tgtcggcgag gatgcgcttc atctcggcct tggtctcggc ggtgacgatg
      301 cgcgggccgg tcttgtagtc gccgtattcg gcggtgttgg agatggagta gcgcatgttg
      361 gcgaggccgc cctcgtagat gaggtcgacg atcagcttca cctcgtggac ggtctcgaaa
      421 taggccatct cgggggcgta gccggcctcg gtcagggttt cgaagccggc ctggatgagg
      481 gcgcagaggc cgccgcagag gacgacctgc tcgccgaaga ggtcggtctc gacctcctcc
      541 ttgaagctgg tctcgatgat cccggcccgg ccgccaccga tggcggcgcc ataggagagg
      601 ccgacgtcat gggcgccgcc ggtggcatcc tggtggatgg cgaggaggca ggggacgccg
      661 gcacccttgg tgtactccgc acggaccaga tggccggggc ccttgggggc gatcatcagg
      721 acgtcgaggt cggggcgcgg ctcgatcagg ttgaagtgga tgttgaagcc gtgggcgaac
      781 atgagggcgg caccctcctt catgttgtcc cgcagctcgt tcttgtagag gtcggcctgg
      841 agctcgtcgg ggacgaggac catgacgatg tcggcccact tggcggcttc gctggcggtc
      901 atgcaccgga agccggcgcg ctcggccttg gcccggctgg cgctgccttc cttcagggcg
      961 acgaccacgt ccgtgacgcc gctgtccttc aggttgttgg cgtggccgaa gccctggctg
     1021 ccatagccga cgacggccac cttcctcgac ttgacgaggt tgagatcggc gtcggtgtcg
     1081 taatagacgc gcatcggggt tccccttcgt tcgtgcgtat cggttcggat ttctggagtt
     1141 cggtcggctc agcgctcgcg cgggcgcagg atggattcgc cgcggccgat ggcgaccacg
     1201 ccggcgcggc tcacctcgat cggctcgacg aggtggatga aggcgtcgac ctcgccggac
     1261 gtgccggtga cctcgaagac gaagctctcg ggcgtgctgt cgacgaggcg ggcatgcatg
     1321 aagtcggcga tgcggagcgc ctggacgagg ttctcgcggc cgccgacgac cttgaccagg
     1381 gccagctcgc gctcgatgtg cgggccctcg acggtgaggt cgagcaccga gtgaaccggc
     1441 accagcttcg ccagcaggtt cctgatctgc tcgatgacca aaggcgtgcc ggtggtcacg
     1501 atgttgatgc gcgactggga cttgtcggcg tcgatctcgg cgaccgtcag gctctcgatg
     1561 ttgtagccgc ggccggagaa gaggccgatg acccgggcga ggacgcccgg ctcgttgtcg
     1621 accaggacga cgatggtgtg acgttcctcg ggcgtccgct cggcatcgga gaaaagggcc
     1681 tgcggggtgg agggcttgga ttctttggcg ggcatgggag gcagtccggc gagaaggggc
     1741 tgggtggcgg tggcgccacc cgggtgtcag gcagggagcc cgggcccttg gcggcccggg
     1801 cctggcatca tacgagcacc atgcccttct cggagatcgc ctcgtgggcg ccgtcgtcct
     1861 cggggctgag cttgatctcg ttgtgggcgg ccccgcccgg gatcatcggg tagacgttct
     1921 cggtccggtc gacgacgatg tcggcgatga ccggctgctt caccttgatc atctccttga
     1981 tgaggtcgtc gacctcggat ggcttctcgg cgcgcaggcc cacggcaccg aaggcctcgg
     2041 ccagcttcac gaagtccggc aggctgtcca tgtagctctc ggcgtgccgg ttgccgtgga
     2101 agaagtcctg ccactggcgg accatgccca tgtagctgtt gttgaggatg aaggacttca
     2161 ccggcaggcg gtactgcatg caggtggaca tctcctgcat gttcatgagc caggaggact
     2221 cgccggcgat gtcgatcacc agggcctcgg ggtgggcgac ctgcacgccc atggcggcgg
     2281 gaaagccgta gcccatggtg ccgaggccgc ccgaggtcat ccagtggaac ggcttgtcga
     2341 aggggaagtg ctgggccgcc cacatctggt gctggcccac ctcggtggtg atgtagacgt
     2401 ccctgtcccg ggtcagctgg tgcagccgct ggatggcgta ttgcggcttg atgctgtcgg
     2461 tgccctggcg aaagcgcagg ctcttcctcg cctgccagtc ggcgatctgc tcccaccagg
     2521 cggcggtgcg gcccagggcc aggctgttgc tgcggcgccg ccaggcggcg agcagggcct
     2581 cgagcgtggg ctcggcgtcg ccgatcaccg gcacgtcgac ggcgacgttc ttgttgatcg
     2641 aggaggggtc gatatcgacg tggatcttcc ggctgccggg cgagaagccg tcgagccggc
     2701 cggtcacccg gtcgtcgaag cgggcgccga tattgatcat cacgtcggcg ccgtgcatcg
     2761 ccatgttggc ttcgtaggtg ccgtgcatgc cgagcatgcc gatgaactgc ctgtcgctcg
     2821 ccggataggc gccgaggccc atcagcgtga gcgtgatcgg gtagccggtc tcccggacca
     2881 gctcggccag cagctcgcag gccctggggc cggcgttgat gacgccgccg ccgacataga
     2941 agatcggccg ctcggcgcgg gtcatgagct cgaccgcctc ctcgatccgg cgcgggtcgg
     3001 ggtcgcgggc cggctggtag gtgcggcgcc gggccgcgtc cttgccctgg tagggggcgg
     3061 gcttcatcag gatgtccttg ggcaggtcga cgacgaccgg gcccggccgg ccgctcctgg
     3121 ccacatggaa ggcctcgtgc accgagcggg cgaggtcctc cgggtccttg acgaggtagt
     3181 tgtgcttggt gcagggccgg gtgatgccga ccgtgtcggc ctcctggaag gcgtcgttgc
     3241 cgatcatgtg ggtcggcacc tggccggtca ggcagacgat cggcacggaa tccatcagcg
     3301 cgtcggtgag gccggtgacg gtgttggtgg cacccgggcc ggaggtcacc aggacgcagc
     3361 cgaccttgcc ggtcgagcgg gcatagccct cggcggcatg gacggcggcc tgctcgtggc
     3421 ggacgaggat gtggcgcagc ttgtcctgct ggaagatggc gtcgtagatc ggcagcaccg
     3481 ccccgccggg atagccgaag atcacctcca cgccctgctc gaccagagcc ttgagcacca
     3541 cctgcgagcc ggtcatcgac tgggtgtcga cggcgggcgg cgctgctgtc gctgcggtca
     3601 tcgatcaaac tccggttgca gcaaaaagag gttgctctat cgctggttcg ttggacgagg
     3661 cgtcattggg cgagcctgcg gcgcagggtc tgccaaacta gtcgggcgct ggcggagaat
     3721 caacagcaca gggcaggccg tggccgcgag cgcggccaca ccgtcgcggt tggatttggc
     3781 cacccggcgc gacaagttgt caagtcgcgt cgcgcaggcc ctccagcagg gccgcggcca
     3841 gggcgtcgag attgtccggc ccgaccgcca ccggatgcgg ctcgagggtt ccggcgagct
     3901 ggcccctgaa gaaggtgcgc tggcgcttgg cgtagcggcg gaccttgatg acggtgagcc
     3961 ggcgcgcctc ggcgaggtcg atccggccgt cgagatgagc gccgagctcg gcgacaccgt
     4021 cggccccgga gagcattgtc ggccggtcgc ccgtccgggc gcggaaggcc gcgagctcgg
     4081 cgagcacgcc ctgctccagc atggcgtcga gccgccgctc gatgcgcggc aggacgaggt
     4141 cggccggcgg caggagggcg attccgacac acggctccgg caagggcggg cggacgggcg
     4201 ggtcgccctg ccagtctgtc agcttccggc cggtggcgag caccacctcg aggccgcgca
     4261 ggatgcgctg gcggtcgccg ggctcgagcc gggcggcaag caggggatcg aggcgggcga
     4321 gccgggcgtg cagcgcctcg gccggggccg ggtcggcgcg gagcccggcc cggaggtccg
     4381 ggggaatttc ggggatcgcc ggcaggcctt ccagcagcgc cttgaagtag aggccggtgc
     4441 cgccggtgag gatggccggc cgcctttgcg cctccagccc ggcgagcacc ggccgcagcc
     4501 gggcgagcca ggcggcgacc gagctcgcct cgtgggcttc gaggatgcca tagaggtggt
     4561 gctcggcgcg cgccagctcg gcggcgggcg gccgggcggt gaggatcggc aggtcccggt
     4621 agagctgcac cgaatcggcg ttgacgatgg ccccgccggt gcgctcggcg agggccaggg
     4681 ccagggccga cttgccgctg gcggtgctgc cgcctatgag cacgaggccg ccggtcacgc
     4741 gcatgtcggc cggctctcga gagccgccat gccgcccacc ggtccgcaat gcctcctcct
     4801 cgtcgccaac ccggcgctcg ccccgctcga accggcgctg gtcgagaccc tcggccggcg
     4861 tcatggcggc accgtccgct ggctcgccga cagcgccgcc gtcgagctgg cgctcgaccc
     4921 ctgcccgccg cgcgagaccc tggccgaggc gctcgccggc cgggcgctgg actgggcgct
     4981 ggtgccggcg gcggggcgga agaagcggct gctggtctcc gacatggaca gcaccatcat
     5041 cgacatcgag tgcatcgacg agctcgccga tttcgtcggc atcaaggcgg aggtggcggc
     5101 gatcaccagg cgcgccatga acggcgagct cgacttcgcg gcagcgctca ccgagcgcgt
     5161 ggcgctgctc gagggcgtgg aagaaaaggc gatcgccgag atctgcaggg aacggctccg
     5221 gctcaacccc ggtgcccgaa cgctcgtctc gaccatgcgc gcggcggggg cgctgaccat
     5281 tctggtctcc gggggcttca cgctgttcac cgccttcgtc gccaggctcg ccggcttcga
     5341 gcggcatcac ggcaaccggc tcgagatccg ggacggccgg ctgaccggcc gggtgctgcc
     5401 gccgatcctc ggtgcgctgg ccaagctcga gacgctggac gaggcggcgg ccgggctcgg
     5461 gctcgaccgc gaggcggcgc tggcgctcgg cgacggcgcc aacgaccggc cgatgctgga
     5521 ggccgccggg ctcgccgtcg cctggcggcc gcaccccgtg ctggcggccg ccgccgacct
     5581 cgtgctcgcc catgccgacc tcgccgccgc cctctacctc cagggctaca cggcggccga
     5641 gatcgccccg agctgattgc tcaggcggcg agtcggagcg ccacgaagcg gaagtcgccc
     5701 tgccggtcga tcaggaggag gacggcgtcg cgcccctcgt cgatcgcctc gttgaccttg
     5761 gccagcacct cgggcggcga gccgacctcg ctctggctga cctcgacgat caggtcgccc
     5821 gggcggagcg cctcctcgtc ggcggcgctg cccggctcga catcgatgat gaccacgccg
     5881 cgcacgtcct ggccgagctc gaaccgggtg cgcatctcgt cgtcgagggc gctgacggtg
     5941 aggccgagct cgtcgatcgc gagcgccgtg ctggccggcg gctcgtcgcc attgccgagc
     6001 tcggacaggg tcgcgtcgtc cggcagctcg ccgagcgtca cccggaccgt ccgctcctcg
     6061 ccacgccgcc agatcagcac gtcgacctcg cggccgacat cggtctccgc cacgatccgg
     6121 ggcaaggacc gcatctgggt gatctcctgg ccgttgaact cgaggatgac gtcgcccgcc
     6181 tggatctccg cgtcctcggc cggaccgccc ggtgtcacgc tggcgaccag cgcaccctgc
     6241 gcctcgtcga gcccgaggct cgccgcgatc tcgtcggtga cgctctggat gcgcaccccg
     6301 agccagccgc gggccacgcg gccatcgctc ttcaggctgt cgatgacccg cttcgcgagg
     6361 ttcgagggaa tggcgaagcc gatgccgaca ttgccgccgg aaggcgagaa gatggcggtg
     6421 ttcacgccga acacctggcc gtccatggtg aagctcggcc cgcccgagtt gccgcggttg
     6481 atcgccgcgt cgacctggaa gaagtcgtcg tagggcccgg cgcggatgtc gcggccccgg
     6541 gccgagacga tgccggcggt gaccgaggtg ccgaggccga acgggttgcc gatggcgagc
     6601 atccagtcgc cgaccctgac ctcgtcgctg tcggcccagt cgacgaacgg gaagggctcg
     6661 tcgcgctcga tcttgagcag ggcgaggtcg gtctgctggt cgcgaccgat cagctcggcg
     6721 ctgtattcct tgccgtcggc gaaggcgacg gtgatctcgt cggcctcggc gatcacgtgg
     6781 ttgttggtga ccacgaaacc gtccggcgcg atgacgaagc cggagccgag cgaggcgccg
     6841 cggcgcggcc gttgcggctg ctgctgctcg cggtcgaaga agtccttgaa gaactcctcg
     6901 aagggcgagc ccggcgggaa ctgcggcagc gggatgtcct cctgcatgac cgcctcacgg
     6961 ctcgtctgga tgttgacgac ggcggggccg acccgttcga ccaggtcgcc gaaaccctgg
     7021 gtaggcagcg tctgcgccac ggccggcacc gatagcggca cctgcgccaa caaggtgaaa
     7081 accagcaacg cggcgagtgg ccgtacgcca gctccggctg ctgcggccgc ccggcgaagg
     7141 ccgaaactca ggtccatcaa ccgctcctat cggcaggccc ggactcctcg tccgagccga
     7201 ttccagcgtt cttggcgagg cccgctcccg cgccctcgcg accgccccaa acgacgcgtg
     7261 ccgcgcccga gacgtgaggc acgcaccgtc aacccgagat gtacgccgtg ccgcgcccgt
     7321 cgccaagccc gctatcgccg tatcaaccag acgatgaaca cgccgagcac cgcgaacgcg
     7381 agcccggccg cccggagcat gccgctctcg agcgtcatgg cgcgggccgc ggcccgcttc
     7441 atggcatccg gaaagagcgc ccagagcgcc ccttcgagca ccagaaccag cgccgaagcg
     7501 gtcagcagat cctgcatcta tgggttctgg atcagcggcc ggggtcgggg cgcgaagaag
     7561 gcccggccgc ccacggccat caggccgcgg gcggccggct ccgtccctat tgggcgctct
     7621 gcggcgccgt cgccggcagc tcggccaccg gcggcacagc cgaggcgtcg gtcgctgctt
     7681 cggctggtgc gcccgctgcc gcgtcggcca ccgccggggg cgccgtcggc gccccggcga
     7741 cctcttccgc gggcagaccc tcgaacgagt tgaagtagcg gaagaactcg ctgtccggtt
     7801 gcaggacgta gctggtcgcg tcgccggcca gggcgttccg gtaggcctgc atcgaccggt
     7861 agaaggcgaa gaagtcggcg tcccggccga acgcctcggc gaaggtgccg atcgccacgc
     7921 tgtcgccacg accacggatc tgctcggctt cgcgacctgc ctcggcgatc agcacgcggc
     7981 gctcgcgatc ggcccgggcg cggatgcgct gggcgatctc cgcaccctgg gcgcgcagct
     8041 cgcgggcctc gcgctcacgc tcggtctgca tccgcaggaa gatggcctgg ctgttctccg
     8101 ccggcaggtc ggcgcgcttg atccgcacgt cgaccacctg cacgccgaac tgctgcagcg
     8161 aggcgttggc ctcgtcgcgg atccggttca tcagccgggt gcgctcgtcg gagaggatgg
     8221 tgaagagcga cacctcgccg agcacgctgc gcagggcgga gaaggtgatc ggctcgagcc
     8281 ggccccggaa cgccgcctcg ctgccgacgc tctggcggaa gcgcaggggg tcggtgatcc
     8341 ggtagcgggt gaaggcgtcg accacgagcc gcttctggtc gcccagcacg agctccaccg
     8401 agccggcgtc gaaatcgagc acgcggcgct cgaaatactc gacctgctgg atgaacggca
     8461 ccttgatgtt gaggccgggc tcggtgacca cccggacggg gttgccgaac tgcaggacca
     8521 gcgcctgctg cgtctggtgg acggtgaaga gggcgctcga caggatgaag aagccggcga
     8581 ccacggcgac cgcgaggccg atgaggagac gatggctcat tgcgtggctc ccgactgcat
     8641 ggcgccgaag ccgcgctcga ccggcgccga gccggcggca gcgctggcgg cagcgtcacg
     8701 ctcgcgcgcc cggcgctcga gatccggcag cggcaaatag gggacgacac cgttgccgcc
     8761 gccctggctg tcgatgatga ccttgttcac gccgcccagc acctcttcca tcgtttccag
     8821 gtagatccgc ttgatggtca cgtccttggc cttgccgtag gcttcgagca cctgggtgaa
     8881 ccggtcggcc tcgccgcggg cgcgctccac cgcctcctgc tcgtaggcct gcgcctcctg
     8941 cagcagccgc tccgcctcgc cacgggcccg cgggatgatg tcgttggcga acgcctcggc
     9001 ctcgttctgc agccgctccc ggtcggcctg ggcgcgctgc acgtcgcgga acgcgtcgat
     9061 gacctcggcc ggcgggtccg acttctggag ctgcacctcc tcgatgttga tgccggcgcc
     9121 gtactcgttc atcagcacct gcatctgctc gcgcacgcgg ccctcgatgg cgcggcggcc
     9181 ctcggtcgtg gcctcgagga tcggcgtctg gccgatcacc tcgcgcatca cgctctcggc
     9241 ggcgatcgac accgtgtcct cgggatcgcg gatgttgaag aggtagtcgg cggcgttgct
     9301 gatccgccag aggacgacga agttcacgtc gatgatgttc tcgtcgccgg tcagcatcag
     9361 cgcttcctcg gtgatgccgc ggccggagcc gctggcgccg gtgccgctgc ggaagccgac
     9421 ctcgacccgg ttgacgcggg tcacctgcgg cgtcagcacc gattcgatcg ggctcggcag
     9481 atgatagttc aggcccggcg tggtgacccg gttgagcttg ccgaagcgca ggacgacgcc
     9541 ttcctcctcg ggctgcaccc gatagaagcc gctggccagc cagatgccga ggagtgccag
     9601 gacgacgatg ctgatcgcct tggccgagag gccgcggccg ccgccacctc cgccgccggg
     9661 aaagaggctc ttgaccttct cctggctctt gcggatcagc tcctcgaggt ccggcggctg
     9721 cgggccgccg ccaccgccgc ccgggcggtt gccccacggc ccctggccgc cgcctccctg
     9781 ccaaccacca ccgccttgac tgttccaggg catactcggg ccacctcgtc gttgtcgtct
     9841 tgcatgtcgg ggagtggatc ggcagtagag aaccgcgtcg gccgatccga ttgcgagata
     9901 cacttagccc actatatcgg tgagcgcacc ccgaggatca accatggacg agctttcacc
     9961 cgaccgtatc cgccaggccc tggccgatgt gcgccatccg acgagcggcc agagcgtcgt
    10021 aaccctaggc atggtcagcg gaatcgtggt caagggtcgc aatgtcggct ttgccctcga
    10081 ggtgccggcc gccgacgcgc aaaagctcga gccgttgcgc cgggcgtgcg aggaggcggt
    10141 gaaggcgctg cccggggtcg gctcctgcac ggtcgtgctg accgccgagc gcggcccgcc
    10201 gcccgacatg caggcgcggc ccgcggcacc ggcgccgacc cgcgccgggc cgcccggcat
    10261 cgacaagatc atcgccgtgg cctccggcaa gggcggtgtc ggcaagtcga cgacggcgat
    10321 caacctcgcc tgcggcctgc gcgccgccgg tctccgcgtc gggctgctcg acgccgacat
    10381 ctacggcccc tccatgccgc gcatggccgg gctctcgggc cggccgacca ccgccgacgg
    10441 caagaagatg cagccgatgg aagtcgccgg cgtcaagctg atgtcgatgg gcttcctcgt
    10501 cgaggaggag acgccgatga tctggcgcgg ccccatggtg cagagcgcct tgcagcagat
    10561 gctcggcgac gtcgcctggg gcgagctcga catcatggtg gtcgacatgc cgcccggcac
    10621 gggcgacgcc cagctcacca tggcgcagaa ggtgccgctg gccggcgcca tcatcgtctc
    10681 gacgccgcag gacatcgccc tgctcgatgc gaggaaggcg atcaacatgt tccgccgggt
    10741 cgacgtgccg atcctcggca tcgtcgagaa catgagctat ttctgctgcc cgaattgcgg
    10801 ccaccggacg gagatcttcg cgcatggcgg ggccagggag tgcgcggcga agtacaatgt
    10861 cgacttcctc gccgagatcc cgctcgacat cgtcatccgc gagacctcgg acagcggccg
    10921 gccgatcgtc gtgagccagc cggactcgcc gcaggcccag gcctacatga acctggcgct
    10981 gaccgtccgc gacaagctcg ccgggccgac cggcgtgaag cccttcccgc gcatcgtcga
    11041 cgcttgagcg ccgggcggcg tcgtcagccc tggaactcga gcgcctcgac ccgggcgcgg
    11101 gcgacggtga tggcccgctc gagccgctcg cgctcgggct cggtcgggtc cctggcgtcg
    11161 ccgaggtcct cctcggcgtc cttgaggctc tggcgggcgt cgtcgaggtt gagctcgccc
    11221 atgggggccg cggcctcggc gaggacgatc aggcccttgg ggttgacctc ggcgaagccg
    11281 ccctcgacga agatgcgctg ctccacctgc tggccctggt agatcgagat cacgccgggg
    11341 cggagcaggg aaagcagcgg cgcgtgctcg acgagcacgc cgaaatcgcc gtcctcgccc
    11401 ggcaccacca ccatgtcggc gtggacgctg gccagcaccc gctcgggcga gaccagctcg
    11461 aacgccagct tgtcggtcga agcctcggcc atgtccgccc cttaccgctc aggccgcttc
    11521 cgcggcgagc ttcttcgcct tctcgatcgc ctcgtcgatg ctgccgacca tgtagaaggc
    11581 ggattccggc atgtggtcgt agtcgccggc gacgatgccc ttgaagccct tgatggtctc
    11641 ctcgaccggc accagcttgc ccgggctgcc ggtgaagacc tcggcgacga agaagggctg
    11701 gctgaggaag cgctggatct tgcgcgcccg ggcgacgacc agcttgtcct cttccgagag
    11761 ctcgtccatg ccgagaatgg cgatgatgtc ctgcagcgcc ttgtagcgct gcagcgtctc
    11821 ctgcacctgg cgggccacgc ggtagtgctc ctcgccgacg acgcccggct cgaggatgcg
    11881 cgaggtcgag tcgagcgggt ccacggccgg gtagatgccg agctcggcga tcgagcgcga
    11941 cagcacggtc gtcgcgtcga gatgcgagaa ggccgtggcc ggcgccgggt cggtcaggtc
    12001 gtcggccggc acgtagatgg cctggacgga ggtgatcgag cccttgttgg tcgaggtgat
    12061 gcgctcctgc aggccgccca tgtcggtgcc gagcgtcggc tggtagccca cggccgaggg
    12121 aatgcggccg agcagcgccg acacttccga gcccgcctgg gtgaagcgga agatgttgtc
    12181 gacgaagaag agcacgtcct ggccttcctg gtcgcggaag tactcggcga gcgtcaggcc
    12241 ggtgaggccg acgcgcgcac gggcgccggg cggctcgttc atctggccgt agaccagcgc
    12301 caccttggac ttgctgcggt cgtcctcgac gatgaccccg gattccacca tctcgtggta
    12361 gaggtcgttg ccctcgcggg tgcgctcgcc gacgccggcg aacaccgaca cgccgccatg
    12421 cgccttggcg atgttgttga tgagctccat gatcaagacg gtcttgccga cgcccgcacc
    12481 accgaagagg ccgatcttgc cgcctttcgg atagggggtg aggagatcca ccaccttgat
    12541 gccggtgacc aggatctcgg cctcggtcga ctggtcgacg aagg
//
LOCUS       DAOTFI010000143        12253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6183_length_12253_cov_6.424742, whole genome shotgun sequence.
ACCESSION   DAOTFI010000143 DAOTFI010000000
VERSION     DAOTFI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_12253_cov_6.424742"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1169
                     /locus_tag="P5558_14790"
     CDS             249..1169
                     /locus_tag="P5558_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY25631.1"
                     /translation="MSRPPVFIDCPPFLDELRRGELGHLVPDLEVHVGDPDPATRSKL
                     LEGRTIVMNDHSQFDRTLMAACPDLKAIVFLGTGAASFVDLAAAADLGITVRNYKGYG
                     DRSVAEHTIAMLFAAVRRIAEMDRAVRAGRFEPIDSFEIRGRKLGLIGTGGIGGEVAR
                     MAAGLGMQVLAWNRSGVDPALPAEAVELDDLLARADIVSLHLALNEETRGFLDAARIG
                     RLKPGAILVNTARGAILDEAALIEALRAGRLRHAALDVFVEEPLPAGHALLGLDNVTL
                     TPHAGFMTREASVNLVRMALELVEEERAKL"
     gene            1205..1963
                     /locus_tag="P5558_14795"
     CDS             1205..1963
                     /locus_tag="P5558_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HRY25632.1"
                     /translation="MPVALVTGARRGIGRGVALRLARSGFDVAVNDVEADADGEAVVA
                     EIRAIGRRALLVPLDIARIDGHDALLDLVEAELGPLACLVNNAGVSVLVRGDLLEVGE
                     ASFDRCLDINLKGTFFLTQAAARHMVRQPAEGHRSIVTISSISAEVVSTSRGEYCITK
                     AGLAMLTKLFALRLAEEGIGVYEVRPGIIRTPMTEPSAERLGQLIEDGFTPIRRWGEP
                     LDVAEAVATLAEGRLPFTVGQVITVDGGLTFRTL"
     gene            complement(2004..3458)
                     /locus_tag="P5558_14800"
     CDS             complement(2004..3458)
                     /locus_tag="P5558_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY25633.1"
                     /translation="MFTEKNGLKHYRHCVDGAWLEGTGRIEVENPATGEIFATVPAGG
                     AAEAALAAEAAARAQPDWEALPPIRRADHLRRLAALIERDGEALARLVTMEQGKPVAE
                     ARGEVQGTALYLTYAAEEARRITGDILPSDEPDEQVWIQRAAHGVVVALTAWNYPLAL
                     TTRKLGPALVTGNTVVVKPHEGTPLSALHIAALVIEAGLPKGVVNVVTGTGRELGDAL
                     VRHPRTALVTLTGSVRAGREVFAAAADGLKVLRLELGGKAPFIIAEDADLDRAVDAAI
                     ASRFENCGQICICNERMYVVDSVADRFMERFLERVRQLKVGDPFDAVDVGPKFSAAEL
                     AKVEAMVEAAKAAGAEVLAGGHRLTDGRFARGHWYAPTVLAGVDHSMDVMRQEIFGPV
                     VPIMRLASFDEALRLSNDTRYGLSAYVFTRDIRRLMRLTRELHFGEIYVNRPGADAVH
                     AHHAGYGDSGLGGEDGKYGIEGYLRKKTVYVNWA"
     gene            complement(3473..4768)
                     /locus_tag="P5558_14805"
     CDS             complement(3473..4768)
                     /locus_tag="P5558_14805"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY25634.1"
                     /translation="MSLAAVLMFAAFFAGAAIGVPIGHAMIATGFLYLWVHGGDMGLV
                     ATQGLNGLFKSFVLLAVPMFIFAAEIMNYPAISDRLLKFTTLLVGRFRGGLAHVNVVL
                     SVIFSGMSGSALADAAGPGKLMISLMTKENRYSPGFAGAVTAASATIGPIIPPSIPMV
                     LYAVVADTSIGYLFMAGIIPGLMMAASLMVLIHVMATRRSFPTEPPIPRSEIVGTVRE
                     ALPTLMLPVILLGGIYSGAVTPTEAAAVAAGYALLLAAGLYRALSPKELFGVLVRSSR
                     ATAIVAVTIIGALFFNYVVAAEGVAEHVANSIAALGLSPLAFLLVINLLFLLLGCFLD
                     TLLMLLIVVPIVMPSVRALGIDTVHFGVVVVVNMMIGLITPPYGQMLFLISGVSGIRL
                     QAMLREIWPFIGALLVALLLMTMLPDVVLLLPRYFGYPG"
     gene            complement(4769..5239)
                     /locus_tag="P5558_14810"
     CDS             complement(4769..5239)
                     /locus_tag="P5558_14810"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRY25635.1"
                     /translation="MLDRLRSFCEIVSAGLFAILFAAFAIQIFTRYVLNDPVVWAQEL
                     CSLAYLWGVCFAAATIVREREHIAFDLVYQAVPPRVRRVLAMLCTGFLVGLFTLGLPG
                     TIDYVLFMGRMKTLDMRIPFDWVFSVFVFFLVVTVLLGLVRLWRLSRADYEREL"
     gene            complement(5257..6282)
                     /gene="dctP"
                     /locus_tag="P5558_14815"
     CDS             complement(5257..6282)
                     /gene="dctP"
                     /locus_tag="P5558_14815"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY25636.1"
                     /translation="MTTKTLAGTALGRRRALALGAAAAAAPFAVRLAGAQEAKLIRIS
                     TPGSAEETLSKSLFVFKEQLEQHAPGTFNVELHFNATLFGQGMEIPAMQRGNLEAAMI
                     SPQDIAEQLPEYSIFTAGYLIQDRDHLERIFAGEVGDEVFARVAEDMGVEILGTQYHG
                     QRHLVLREPKEVNTPADLAGMKLRVPGSESWRFLGAALGGNPTPIAFDEVYLALKTGT
                     VDAVEQPLSDMISAKFYEVTEQLVLTGHLVSTLFFGFSKPFIDSLTDEQRAQVQAAAD
                     AAATWNNDQVSEIEKGAVDFLVAQGMTATTPDLEAFRTHVLDEYLNSDFSANWPEGML
                     EKINNAA"
     gene            6425..7342
                     /locus_tag="P5558_14820"
     CDS             6425..7342
                     /locus_tag="P5558_14820"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY25637.1"
                     /translation="MPAGYAPELIAAAATPAAPDGSCLGALHLRHLRDLLDRGCDAVA
                     LFGTTGEGPAFTVAERRAGLERVLHAGVAPERLVVAAMSAAPGDVLELCRHAANAGVT
                     RLLVMPPFFFRGAASPDGLLRFYGDLVEALGNHHLRIMLYHFPDMSGAVITAELALEL
                     RRRFGAVIDGIKDSAGDLEASLALIRALPAFRVLVGTEVHLPAAVAAGGGGTICGLAN
                     VAPELVRELMLATDPATIAPLTARLDALDALLSAAPFAASLKAMLALTTGEPAWALPQ
                     APMAALGAEATRRLQDGLRQGRWLAEARA"
     gene            complement(7415..8263)
                     /locus_tag="P5558_14825"
     CDS             complement(7415..8263)
                     /locus_tag="P5558_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY25638.1"
                     /translation="MSNDIPPQSQDRQPGLESEMDPRPEFEPRFAGSDRLAGKVALIT
                     GGDSGIGRACAVAMAREGADIAIVYLDETEDAKKICRLVEKEGRRCLAMAGDVGSESF
                     CQEAVGKTVATFGRLDVLVNNAAEQHVTKEPLEIEASQLERTFRTNVFGYFFMTKAAL
                     REMQPGACIVNTTSVTAYRGSKELIDYAATRGAIVAFTRSLAKALVERKIRVNGVAPG
                     PIWTPLIPASFDPKRVAEHGGSVPMQRPGQPNEVASCHVFLASEEASYMTGQVLHPDG
                     GGDMSS"
     gene            complement(8333..9388)
                     /locus_tag="P5558_14830"
     CDS             complement(8333..9388)
                     /locus_tag="P5558_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HRY25639.1"
                     /translation="MPSRPRLDEQPSRGAPVTTLVYVDDESGLRIRRRRCGRGFVYVD
                     AAGARVTNAEESRRIRALAIPPAWTDVRICADPAGHIQASGRDSRGRKQYCYHADWQH
                     VRDAAKLQELVRFGEALPRLRVRVRRDLRRQGLCRSRVLALVVDLLDRTLIRVGSDIY
                     ARQNGSFGLTTLTKHHVAARGEKLRFRFTGKSGKQWDVGLADRRLARVVRRCQELPGQ
                     RLFQYTDGDGAVHAVDSADVNAYLREIGGEGVTSRRFRTWAATVGVANRLVGEPAPRT
                     RREETRAINRALDEMAVRLGNTRAICRKSYVLPAVLESFAAGELGAAFARMAGYRRRG
                     LTAAEARLLAWLRERQA"
     gene            complement(9589..10254)
                     /locus_tag="P5558_14835"
     CDS             complement(9589..10254)
                     /locus_tag="P5558_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25640.1"
                     /translation="MSVMGTGAVAIWHDIAAEGRDEFYAWHGQEHMPERVGIKGFIRG
                     RRWIAIEADREYFNLYETQGPEVVRGPDYKARLDAPTPWTLATVKHFRAVARSLCRVE
                     ASVGMVDGGLAATLRLDLPDEAGAGTWPARLLDLMTLPGIAAASLVIADRAASGYVNA
                     EQRERGGANEIPTHAVLVEGWGDEAPFMAAIRDALGDAGVLGFYRHQITVSRAPASTA
                     GQA"
     gene            complement(10307..11188)
                     /locus_tag="P5558_14840"
     CDS             complement(10307..11188)
                     /locus_tag="P5558_14840"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25641.1"
                     /translation="MSASSSSTIEAAPARTPSAVEPPTGVAADRVAAPAAAGSPISVR
                     GVSHVFRRGSLVTHALDRVDLEIEAGEFVAVLGPSGCGKSTLLKILAGLLDHTTGEIE
                     ISGKPVTGPQTGLGIVFQSPVLLDWRTILDNVLVQIELRGLDRRRYLDPALDLLRKVG
                     IEEFKDRLPRELSGGMRQRAAIVRSLIHDPPLLMMDEPFGALDALTREQMRIDLEKLW
                     LERRHTTVFITHGISEAVALADRVVVMTPRPGRIDHIIEIDLPRPRDKAVITSSRFAA
                     HCDEITESFMRHGVIRY"
     gene            complement(11189..12148)
                     /locus_tag="P5558_14845"
     CDS             complement(11189..12148)
                     /locus_tag="P5558_14845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25642.1"
                     /translation="MIAAAAAALMLGGAWTAEAADEVSLRLNWYLGGLHVPFYYGKEQ
                     GIFEAEGIDLTINEGRGSGNTTQVVAAGSDTFGMADSSSLITLASKGGEIKSVMSMLN
                     STGFSVVSLAETGIREPKDLEGKSVAVSPGDPLAQLLEAVAKANDVDISTINFIQVDP
                     AAKVVSVLEGRADALLGGADDQYFLIKYQGKEPHALRYSEHGANIVGMTILTRTDLIE
                     SNPDLVERFVRASVKSWEAAIENPEAAVEAALKVKPDLNEQSTLDQLMVDIELLHSPA
                     SPDRIGYGAEEDWTRTIDLLKTYRGLETDQDWTAFHTNEFVPE"
BASE COUNT         1866 a         4101 c         4379 g         1907 t
ORIGIN      
        1 tcctcgtgac acaactctca cgttgctcat tcgatcgaca taccgaccat tgagtttcat
       61 aggctagctt acattagctt gaaaagagac aatcgtcgct cccttggtgg gccatggcgg
      121 catcgcgtgc atcgcgggga cgcaccaacg gggctgcgcc gggccgcagc agccgctaga
      181 acgggcgagc cgccggcggc gatgggtcgc cgacgtgatg gtcactgctg actcgaggaa
      241 cccgcctgat gtcccgtccg cccgtgttca tcgactgccc ccccttcctc gacgagttgc
      301 gccggggcga gctcggtcat ctggtgcccg atctcgaggt gcatgtcggc gaccccgatc
      361 cggcgacacg aagcaagctg ctcgagggcc ggacgatcgt catgaacgat cactcgcagt
      421 tcgaccgcac gctcatggct gcctgcccgg acctgaaggc catcgtcttc ctcggcacgg
      481 gtgccgcgag cttcgtcgat ctggcggctg cggccgatct cggcatcacc gtgcgcaact
      541 acaagggcta tggtgacagg agtgtcgccg agcacacgat cgccatgctc ttcgccgccg
      601 tccggcggat cgccgagatg gaccgggcgg tgcgggccgg caggttcgag ccgatcgaca
      661 gcttcgagat ccggggccgg aagctgggcc tcatcgggac gggcggcatc ggcggcgagg
      721 tggcgcggat ggccgccggc ctcggcatgc aggtgctcgc ctggaaccgc agcggcgtcg
      781 atccggcgct gccggccgag gcggtcgagc tggacgatct cctggcgcgg gcggacatcg
      841 tctccctgca tctggcgctc aacgaggaga cgcggggctt tctcgacgcg gcgcggatcg
      901 gccggctgaa gccgggtgcg atcctggtca acacggcgcg cggcgccatc ctcgacgagg
      961 cggcgctgat cgaggcgctg cgggcgggcc gactgcggca tgcggcgctc gacgtcttcg
     1021 tggaggagcc gctgccggcg ggccacgcgc tgctcggcct cgacaacgtg acgctcaccc
     1081 cgcatgccgg gttcatgacc cgcgaggcct cggtcaatct cgtgcgcatg gcgctcgagc
     1141 tggtcgagga ggagcgggcg aagctctgag accggcgtcg ggcagctgac ggagagatct
     1201 ggccatgccg gtggcactgg taacgggggc gaggcgcggg atcgggcgag gcgtggcgct
     1261 caggctcgct cggagcggct tcgatgtggc ggtcaacgac gtcgaggccg acgcggacgg
     1321 cgaagctgtg gtggcggaga tccgggccat cggcaggcgg gcgttgctgg tgccgctcga
     1381 catcgcccgg atcgacgggc acgacgcgct tctcgacttg gtcgaggccg agctcggccc
     1441 cctcgcctgc ctcgtcaaca atgcaggggt ttcggtcctg gtgcgcggcg acctgctgga
     1501 ggtcggcgag gcgagcttcg accgctgcct cgacatcaac ctcaagggca cgttcttcct
     1561 cacccaggcg gcggcacggc acatggtgcg gcagccggcc gaggggcacc ggtcgatcgt
     1621 caccatctcg tcgatcagtg ccgaggtggt ctcgaccagc cggggcgagt actgcatcac
     1681 caaggccgga ctcgccatgc tcacgaagct cttcgcgctg cggctcgccg aggagggaat
     1741 cggcgtctac gaggtgcggc ccggcatcat ccggacaccg atgaccgagc cgtcggccga
     1801 gcgtctcggc cagctcatcg aggacggctt cacgccgatc cgccgctggg gcgagcctct
     1861 ggacgtggcc gaggcggtcg ccacattggc cgaagggcgc ctgcccttca ccgtcggtca
     1921 ggtgatcacg gtggacggcg ggctgacctt caggacgctc tgattggggc ggttatcggc
     1981 cttcggcctg ctcgagccgt ggttcaggcc cagttgacgt agacggtctt cttcctgagg
     2041 tagccctcga tgccgtactt gccgtcctcg ccgccgaggc cgctgtcgcc gtagcccgcg
     2101 tggtgcgcgt ggacggcgtc ggcgccaggt cggttgacgt agatctcgcc gaaatgcagc
     2161 tcacgcgtga gccgcatcag ccgccggatg tcgcgggtga agacataggc cgagaggccg
     2221 tagcgcgtgt cgttggagag cctcagcgcc tcgtcgaagc tggcgagccg catgatcggc
     2281 accacggggc cgaagatctc ctggcgcatc acgtccatcg agtggtcgac gccggcgaga
     2341 accgtcggcg cgtaccagtg gccgcgggcg aagcggccgt cggtcaaccg gtgcccgccg
     2401 gccagaacct cggcaccggc ggctttcgcg gcctcgacca tggcctcgac cttggcgagc
     2461 tcggcggcgc tgaacttcgg gccgacatcg accgcgtcga agggatcgcc caccttcagc
     2521 tggcggaccc gttcgaggaa gcgctccatg aagcggtcgg cgacggaatc caccacgtac
     2581 atgcgctcgt tgcagatgca gatctggccg cagttctcga agcgggaagc gatggcggcg
     2641 tcgacggcac gatcgagatc ggcgtcctcg gcaatgatga agggtgcctt gccgccgagc
     2701 tcgaggcgca gaaccttgag gccgtccgcg gcggcggcga acacctccct gccggcgcgc
     2761 acgctgccgg tcagcgtgac gagggcggtt cgggggtggc gcaccagcgc gtcgccgagc
     2821 tccctgcccg tgccggtgac gacgttgacg acacccttcg gcaagccggc ctcgatgacg
     2881 agggcggcga tgtgcagggc cgagagcggc gttccctcgt gcggcttgac cacgaccgtg
     2941 ttgccggtga ccagcgccgg cccgagcttg cgcgtcgtca gtgctagcgg atagttccag
     3001 gcggtgagcg cgacgaccac gccatgggcg gcgcgctgga tccagacctg ctcgtcaggc
     3061 tcgtccgagg gcaggatgtc gccggtgatc cgccgcgcct cctcggcggc gtaggtgagg
     3121 tagagggcgg tcccctggac ctcgccccgc gcctcggcga cgggcttgcc ctgctccatg
     3181 gtgacgagcc gggcgagggc ttccccgtcg cgttcgatca gggcggcgag ccggcggaga
     3241 tggtcggcac gacggatcgg cggcagggcc tcccagtcgg gctgggcacg ggccgcggcc
     3301 tcggcggcca aagcggcctc ggcggcgccg ccggccggca cagtggcaaa gatctcgccg
     3361 gtagccggat tctccacctc gatcctgccg gtgccttcca gccaagcgcc gtcgacgcaa
     3421 tggcgatagt gcttcaggcc gttcttctcg gtgaacatgt gctgggctcc ggtcagccgg
     3481 gatagccgaa atagcggggc agcaggagga cgacgtcggg gagcatcgtc atcaggagaa
     3541 gggccaccag gagggcgccg atgaagggcc agatctcgcg cagcatggcc tgcaggcgaa
     3601 tgccgctgac cccgctgatg aggaagagca tctggccgta gggcggggtg atgaggccga
     3661 tcatcatgtt gacgacgacg accacgccga aatggacggt gtcgatgccg agtgccctga
     3721 ccgacggcat gacgatgggc accacgatca gcagcatgag aagggtgtcg aggaagcacc
     3781 ccagcagcag gaagagcagg ttgatgacca ggaggaaggc gagcggcgag aggcccaggg
     3841 ctgcgatcga gttggcgacg tgctcggcga ctccctcggc ggcgacgaca tagttgaaga
     3901 agagggcgcc gatgatggtg acggcgacga tcgccgtggc gcgcgaggag cgcaccagca
     3961 cgccgaagag ctccttcggc gacagggcgc ggtagagtcc ggcggcgagc aggagcgcat
     4021 agccggcggc gacggcggcc gcctcggtcg gggtgacggc acccgagtag atgccaccca
     4081 gcaggatcac cggcagcatg agggtcggca gcgcctcgcg caccgtgccg acgatctcgc
     4141 ttctcgggat cggcggctcg gtcgggaagc tccggcgcgt cgccatgaca tggatcaaga
     4201 ccatgagcga ggctgccatc atcaggcccg ggatgatgcc ggccatgaag agatagccga
     4261 tcgaggtgtc ggcgacgacg gcgtagagca ccatcgggat cgagggcggg atgatcgggc
     4321 cgatggtggc ggaagccgcg gtgaccgccc cggcgaagcc tggtgaatag cggttctcct
     4381 tggtcatcag gctgatcatc agcttgccgg ggccggccgc atcggcgagg gcactgccgc
     4441 tcatgccgga gaagatgacg ctgagcacca cgttgacgtg ggcgaggcca ccccggaagc
     4501 ggccgaccag aagtgtggtg aacttgagca ggcggtcgct gatcgccggg tagttcatga
     4561 tctcggcggc gaagatgaac atcggcacgg cgagcaggac gaagctcttg aagaggccgt
     4621 tcaggccctg ggtcgcgacc aggcccatgt cgccgccatg gacccagaga tagaggaagc
     4681 ccgtggcgat catggcgtgg ccgatcggca caccgatggc ggcgcctgcg aagaaggcgg
     4741 cgaacatcag gacggcggcg aggctcattc agagctcgcg ttcgtagtcg gcgcggctca
     4801 gccgccagag ccgcacgagg ccgaggagca cggtcacgac aaggaagaag acgaagacgg
     4861 agaacaccca atcgaacggt atgcgcatgt cgagggtctt catccggccc atgaagagca
     4921 cgtaatcgat ggtgccgggc aggccgagcg tgaagagccc gacgagaaag cccgtgcaga
     4981 gcatggcgag cacgcgccgc acgcgcggcg gcaccgcctg gtagacgagg tcgaaggcga
     5041 tatgctcacg ctccctgacg attgtggcgg cagcgaagca gaccccccag agataggcca
     5101 gcgagcagag ctcctgcgcc cagaccaccg gatcgttgag gacgtagcgg gtgaagatct
     5161 ggatggcgaa ggcagcgaac agaatggcga aaaggccggc gctgacgatc tcgcagaagc
     5221 tgcgcagacg gtccagcacc ggccggtacc gcggcctcag gcggcgttgt tgatcttctc
     5281 gagcatccct tccggccagt tggcggagaa atcggagttc aggtactcgt cgaggacgtg
     5341 ggtgcggaag gcctcgaggt cgggcgtggt cgcggtcatg ccctgcgcga cgaggaagtc
     5401 gaccgccccc ttctcgatct cgctgacctg gtcgttgttc caggtcgcgg cggcgtcggc
     5461 ggccgcctgg acctgggcgc gctgctcgtc ggtcaggctg tcgatgaagg gcttggagaa
     5521 gccgaagaag agggtcgaga ccaggtggcc ggtgagcacg agctgctcgg tcacctcgta
     5581 gaacttggcc gagatcatgt cggagagggg ctgctcgacc gcgtccaccg tgccggtctt
     5641 gagcgccagg tagacctcgt cgaaggcgat gggcgtcggg ttgccgccga gcgccgcacc
     5701 gaggaagcgc cagctttcgg agcccggcac gcgcagcttc atgccggcga ggtcggccgg
     5761 cgtgttgacc tccttgggct cgcgcagcac gaggtggcgc tggccgtgat actgcgtgcc
     5821 gagaatctcc acacccatgt cctcggcgac cctggcgaac acctcgtcgc cgacctcgcc
     5881 cgcgaagatg cgctcgagat gatcgcggtc ctgaatcagg tagccggcgg tgaaaatcga
     5941 gtactcgggg agctgctcgg cgatgtcctg cggcgagatc atcgccgcct ccaggttgcc
     6001 gcgctgcatg gccgggatct ccatgccctg gccgaagagc gtggcattga aatgaagctc
     6061 gacgttgaac gtccccggcg cgtgctgctc gagctgctcc ttgaagacga agagcgactt
     6121 ggagagcgtc tcctccgcgc tgcccggcgt gctgatgcgg atcagcttgg cctcctgcgc
     6181 cccggcaagc cgaacggcga agggtgctgc cgccgccgct gcccccaggg cgagcgcacg
     6241 gcgccggccg agagcggtcc cggcgagagt cttggtcgtc atttcttgtc cccctgaacg
     6301 tggccggcgg cgcccttccg gcgcgccggt tctggcttgc ggcgatcatt gacaatggcg
     6361 atccggctgg tcaagcaggg aatgcccgtt cccagcaccc ggcccgggag gccgcccgcc
     6421 gtccatgccc gccggatacg cacccgagct catcgccgct gccgccacgc ccgccgcacc
     6481 cgacggcagc tgcctcggcg ccctgcattt gcgccatctg cgcgacctgc tcgaccgcgg
     6541 ctgcgacgcc gtggccctgt tcggcaccac cggcgagggt ccggccttta ccgtggccga
     6601 gcgccgggcg ggactcgagc gggtgctgca cgccggcgtc gcaccggagc ggctggtggt
     6661 cgcggcgatg tcggcggcgc cgggcgacgt gctcgagctc tgtcggcacg ccgccaatgc
     6721 cggggtgacg cggctgctgg tgatgccgcc cttcttcttc cgcggtgccg ccagccctga
     6781 cgggctgctg cggttctacg gcgatctcgt cgaggcgctg ggcaaccatc acctgcgcat
     6841 catgctctac catttcccgg acatgagcgg agcggtgatc accgccgagc tggcgctgga
     6901 gctgcgccgg cgtttcggtg ccgtgatcga cggcatcaag gacagcgccg gcgatctcga
     6961 ggcgagcctc gccctgatcc gcgccctgcc cgcgtttcgc gtccttgtcg gcacggaagt
     7021 gcatttgccg gcggcggtgg ctgccggcgg cggcggcacc atctgcggcc tcgccaatgt
     7081 ggcgcccgag ctggtccgcg agctgatgct ggccaccgac cccgccacga tcgcgcccct
     7141 gacggcccgg ctggatgcgc tcgacgccct tctgagcgct gcgcccttcg cggcctcgct
     7201 caaggcgatg ctggcgctga ccaccggcga gccagcctgg gccctgcctc aggcgcccat
     7261 ggcggcgctc ggcgccgagg cgacgcggcg gctgcaggac gggctgcgcc aagggcgatg
     7321 gctcgcagag gcccgagcct gatgctggga gcgggtgtcg cggcggaaca ggccagtgcc
     7381 gcgcccggga gaatgcgcgc ctcgcgcctg ccgctcagga cgacatgtcg ccgccgccgt
     7441 cggggtggag cacctgaccg gtcatgtatg acgcctcctc gctggcgagg aagacgtggc
     7501 aggaggcgac ctcgttgggc tggccgggcc gctgcatcgg cacgctgccg ccgtgctcgg
     7561 cgacccgttt tgggtcgaac gaggccggga tgagcggcgt ccagatgggg ccgggcgcca
     7621 ccccgttgac acggatcttc cgctccacca gggccttcgc cagcgagcgg gtgaaggcga
     7681 cgatcgcccc ccgtgtcgcc gcgtagtcga tcagctcctt gctgccgcga taggcggtga
     7741 ccgaggtggt gttgacgatg caggcccccg gctgcatttc gcggagcgct gccttggtca
     7801 tgaagaagta gccgaagacg ttggtgcgga acgtgcgctc gagctggctc gcctcgatct
     7861 cgagcggctc cttcgtgacg tgctgctcgg cggcgttgtt caccagcacg tcgagccggc
     7921 cgaacgtcgc gaccgtcttg cccacggcct cctggcagaa gctctccgag ccgacatcgc
     7981 ccgccatcgc gaggcaccgg cgaccctcct tctcgaccag acggcagatc ttcttcgcat
     8041 cctccgtctc gtcgagatag acgatcgcga tatcggctcc ctcgcgcgcc atggcgacgg
     8101 cgcaggcgcg gccgatgccg ctgtcgccgc cggtgatcaa tgccaccttg ccggcgaggc
     8161 ggtccgagcc ggcgaagcgc ggctcgaact cgggcctcgg gtccatttca ctctccaggc
     8221 ccggctgtcg gtcctggctc tggggcggta tgtcgttgct catgatctgc acctccgacg
     8281 tgcaccttcg cgttccgcca tcaacgcagc ccgttgcctc gggttccgga agtcaggcct
     8341 gccgctcccg gagccaagcc agcagcctcg cctcggccgc cgtcagcccg cgccggcggt
     8401 agcccgccat ccgggcgaac gcggcgccga gctcaccggc cgcgaagctc tcgagcaccg
     8461 ccggcaggac gtagctcttg cggcagatgg cgcgcgtgtt gccgagccgg acagccatct
     8521 cgtccagcgc ccggttgatt gcccgcgtct cttcccgcct cgtccggggt gccggctcgc
     8581 cgaccagccg gttggcgacg ccgaccgtcg cggcccaggt gcgaaagcgc ctggatgtca
     8641 cgccctcccc gccgatctcg cggaggtagg cgttcacgtc cgccgagtcg acggcgtgaa
     8701 ccgcaccatc gccgtcggtg tactgaaaca gccgctggcc cggcagctcc tggcaccgcc
     8761 gaacgacccg agccagccgg cggtcggcga ggccgacgtc ccattgcttg ccggacttgc
     8821 cggtgaagcg aaagcgcagc ttctcgccgc gggcggcgac atggtgcttg gtcagggtgg
     8881 tgaggccgaa gctgccgttc tgcctcgcgt agatgtcgct gccgacacgg atgagcgtgc
     8941 gatccaggag atccacgacg agggccagga cccggctgcg acagagaccc tgccggcgca
     9001 gatcgcgtcg caccctgact cgaagtcgcg gcagcgcctc gccgaaccgc accagctcct
     9061 ggagcttcgc cgcatcgcgc acatgctgcc agtccgcgtg gtagcagtac tgcttgcgtc
     9121 cccggctgtc gcgaccgctg gcctggatgt ggccggcggg atcggcgcat atccgcacgt
     9181 ccgtccatgc cggcgggatc gcgagggcgc ggattcgcct gctttcctcg gcattcgtca
     9241 ccctggcacc ggcggcatcg acatacacga agccgcggcc gcagcgacga cggcgaatgc
     9301 gcaggccgct ctcgtcgtcc acatagacga gggtggttac gggtgcgccg cgtgacggct
     9361 gctcgtcgag ccggggtcga gatggcattg cgccctccgc aggcgcgcgt gtcacctcgc
     9421 gcgcttcgcc agggctcgaa ccggcgcacc gccgcagcgg ttcctcgcgc cctgcagtgc
     9481 cacgccggcg ccgctcgtca ggaacctccc atgccagccg ggccgagggc gcaacggaag
     9541 gttgccggtg cggcgctcgc cgcccggccg gtcgacctgt ccagcgcgct acgcctggcc
     9601 cgcggtcgag gccggtgcgc gcgagaccgt gatctggtga cgatagaagc cgagaacgcc
     9661 cgcgtcgccg agggcgtctc gaatcgccgc catgaaaggc gcctcgtcgc cccacccttc
     9721 caccaggacc gcatgggtcg ggatctcgtt ggcacccccg cgctcgcgct gctcggcgtt
     9781 gacatacccg ctggcggccc ggtcggcgat caccagcgac gctgcggcga tgccgggcag
     9841 ggtcatgagg tcgagcagcc gggcgggcca ggtacccgcg ccggcttcgt cgggtagatc
     9901 cagccgaagc gtcgcggcga ggccgccatc gaccatgccc acgctcgcct cgacccggca
     9961 aagcgaacgg gcgacggcgc ggaaatgctt cacggtggcg agcgtccagg gggtgggcgc
    10021 gtcgagccgg gccttgtaat ccggcccccg gacgacctct ggaccctgtg tctcgtagag
    10081 attgaagtac tcgcggtccg cctcgatcgc gatccagcgg cgaccgcgga tgaagccctt
    10141 gatgccgacc cgctccggca tgtgctcctg gccatgccag gcgtagaact cgtcacgccc
    10201 ctcggcggcg atatcgtgcc agatggcgac ggcaccggtt cccataacgc tcatcgtctc
    10261 gtcatcctcg aatgctgggc atgctgcaga tcgaaggggc gccgggtcag tagcgaatga
    10321 cgccgtggcg catgaagctc tcggtgatct cgtcgcaatg ggcggcaaag cgcgagctgg
    10381 tgatcaccgc cttgtcgcgc ggcctgggca ggtcgatctc tatgatgtgg tcgatccggc
    10441 cgggccgcgg tgtcatgacc accacgcggt cggcgagcgc caccgcctcg ctgatgccgt
    10501 gggtgatgaa gacggtggta tggcgccgct cgagccagag cttctcgagg tcgatgcgca
    10561 tctgctcacg ggtcagcgcg tcgagcgcgc cgaagggctc gtccatcatc aggagcggcg
    10621 ggtcgtggat cagcgagcgg acgatcgccg cccgctggcg catgccgccg gagagctcgc
    10681 gcggcaggcg gtccttgaac tcctcgatgc cgaccttgcg caggagatcg agggccgggt
    10741 cgagataacg gcggcggtcg agcccgcgca gctcgatctg caccagcacg ttgtcgagga
    10801 tggtccgcca gtcgagcagc acggggctct ggaagacgat gccgagcccg gtctgcggcc
    10861 cggtcacggg cttgccggaa atctcgatct cgcccgtcgt gtggtcgagc agcccggcca
    10921 ggatcttcag gagcgtgctc ttgccgcagc cggacgggcc gagcaccgcc acgaactcgc
    10981 cggcctcgat ctcgagatcg acgcggtcga gggcatgggt gacgagggag ccgcgccgga
    11041 agacgtggct tacgccgcgc acgctgatcg gcgagccggc ggcggccgga gccgccaccc
    11101 tgtccgctgc cacaccggtc ggcggctcga ccgccgacgg tgtgcgggcc ggggcagcct
    11161 cgatggtgct gctcgacgat gcgctcatct actcgggcac gaactcgttg gtgtggaacg
    11221 cggtccagtc ctggtcggtc tccaggccgc gataggtctt caggaggtcg atcgtgcgcg
    11281 tccagtcctc ctcggcgccg tagccgatac gatcgggcga ggctggcgag tgcagcagct
    11341 cgatgtcgac catgagctgg tccagggtcg actgctcatt gaggtcgggt ttgaccttca
    11401 gggccgcctc gacggcagcc tccgggttct cgatcgccgc ctcccaggac ttcaccgagg
    11461 cccgcacgaa gcgctcgacc aggtcggggt tgctctcgat caggtcggtc cgggtgagga
    11521 tggtcatgcc gacgatgttg gcgccatgct ccgagtagcg cagcgcgtgc ggctccttgc
    11581 cctggtactt gatgaggaag tactggtcgt cggcgccgcc gaggagcgcg tcggcgcgtc
    11641 cctccagcac gctcaccacc ttggcggccg ggtcgacctg gatgaagttg atggtcgaga
    11701 tgtcgacgtc gttggccttg gcgacggctt cgagcagctg ggccaggggg tcgcccggcg
    11761 agaccgccac gctcttgccc tcgaggtcct tgggctcgcg gatgccggtc tcggcgagcg
    11821 agacgaccga gaagccggtc gagttcagca tcgacatcac cgacttgatc tcgccgccct
    11881 tggaggcgag cgtgatcagg ctcgacgagt ccgccatgcc gaacgtgtcg gagccggcgg
    11941 cgaccacctg ggtcgtgttg ccggagccgc ggccctcgtt gatggtgagg tcgatgcctt
    12001 ccgcctcgaa gatgccctgc tccttgccgt agtagaaggg cacgtgcagc ccaccgagat
    12061 accagttgag ccggagcgaa acctcgtccg cggcctccgc cgtccaggcg ccgcccagca
    12121 tcagcgcggc ggccgccgct gcgatcatcc ttttcgtctt cattcctctg cccccctcgg
    12181 gtggttgttt tcggtcctag actcccgtca ccccggccgg ccgctgcgac acgtgccagg
    12241 gaatgacgag ccg
//
LOCUS       DAOTFI010000144        12193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6217_length_12193_cov_7.301368, whole genome shotgun sequence.
ACCESSION   DAOTFI010000144 DAOTFI010000000
VERSION     DAOTFI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6217_length_12193_cov_7.301368"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="P5558_14850"
     CDS             <1..218
                     /locus_tag="P5558_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY25643.1"
                     /translation="VLDVDMAGERSMPIVAALKARGVPFVLVTGYSAMQLPEAEFEGA
                     TRIGKPYGDGELVRAIRAMLGSPTPPR"
     gene            complement(202..1485)
                     /locus_tag="P5558_14855"
     CDS             complement(202..1485)
                     /locus_tag="P5558_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY25644.1"
                     /translation="MERAVIRHAAVWDGESKSTFKAEVEIAGERIARIARDGASIGAE
                     GATVIDGSGMTLMPGMVEGHCHPSFTGINAPFELGLIPPEEHMLLTAKNCRLLLDHGF
                     TSIFEAASAKPLLGVTARNAINSGLLAGPRMRAGSPEITVTAGLGDERKRHIHQESFG
                     LVADGPDEMRRVVRECIRDGVDTIKINISGDEFVSHARAEITPLTDAELATVMEVAHG
                     FGKKVAAHARSSESVKMAVRHGVDCIYHCDFADEEALDLLESVKDTAFVGPAFGLVHN
                     CTKEGEGIGLSPEVCRQLDLYRKFDACCATYHEIRKRGIRVVIGGDYGFAVTPMGQNA
                     RDIEHFVEYFGYAPAEALRCATVVGAELMGMGAELGRVKEGFIADLLLVRGNPLEDVS
                     LVQHQANLAMIMQAGRLYKDPRGLASTDRHLIAAE"
     gene            complement(1687..2319)
                     /locus_tag="P5558_14860"
     CDS             complement(1687..2319)
                     /locus_tag="P5558_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25645.1"
                     /translation="MFFLVAVSCLSSCTAWPVPSPPISTLEVPPAAEIEPLRPGETLA
                     VLADDRMASGWSTDCLTGYLVEVLSKARVLDDSAVRDAFFPWLETAQSIPVSEAAASE
                     FLARPRVAAKLRELGLRYILFVRHGVEAKRDPWRSQSLAAFNEVVDRIAVTAVELETA
                     CFRVGGAARAAGIEGHFVVPIFGFVLLSPTESAACARLASALLVELRPAD"
     gene            complement(2327..2845)
                     /locus_tag="P5558_14865"
     CDS             complement(2327..2845)
                     /locus_tag="P5558_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25646.1"
                     /translation="MLEPAPGTGWLPPLIEPLPLRLSYVFEPSVDQEVTFSAVSSGTL
                     TTHLRLGPATRAIFERTLPALVESARAGPPRPGDNGSLTIALLGVEVTKDAWSLRNYV
                     VAWSVDYGITFVDPDGTDIGTWKISGATVYDGSEEALAQTLRGATAELVRSFAVQPAI
                     VAWLTARREAMT"
     gene            complement(3025..3648)
                     /locus_tag="P5558_14870"
     CDS             complement(3025..3648)
                     /locus_tag="P5558_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25647.1"
                     /translation="MRTWRRSLAGLLLLAGLGPVPAARAGMLVEALFDDVPLRLETGS
                     DHGRVLVTVAGEHYLVDLAPQNVYRLDGGAPTLIRAGLADDGASALPYVLDHWSAGPP
                     VAGHASTYHVLQIDEQICGEVLASAWMTPFLEPVVRSLELMQRVAPALRPVQRENCGA
                     IPFTVYARNGWPLMAGWRDEAAFLTQTVRFDHYPDDARFARPGDTVH"
     gene            complement(3702..5018)
                     /gene="purB"
                     /locus_tag="P5558_14875"
     CDS             complement(3702..5018)
                     /gene="purB"
                     /locus_tag="P5558_14875"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRY25648.1"
                     /translation="MIPRYSRPQMVAVFAPERRLELWLEIELLVAEAMAGLGQIDGEA
                     VARLRTATEQARDRLIDAAAVEAIEATTRHDVIAFLTHVENLCGADARLLHLGMTSSD
                     LLDTTLALQLREAGQLVLADLDALLAVLARRAREHAMDVCIGRSHGIHAEPTTFGLKL
                     AGFHAAFARQRQRLLAAIEDVSTCAISGAVGTFANIDPAIEARVAGKLGLRVEPVSTQ
                     VIPRDRHAAFACALAQIAGSIEHLATEIRHLQRSEVREVEEFFHAGQKGSSAMPHKRN
                     PVLSENLTGLARMIRAAVVPALENVALWHERDISHSSVERVMLPDSCILADFALARLA
                     GLMDRLVVHPERMRRNLDATHGLFNSQTVLLALVDAGMGRQAAYELVQASAMRAWENE
                     EPLLDLLEVDPRITAVLDEKSLADLFDPARHTRHVPMLLERALAVS"
     gene            5146..7005
                     /locus_tag="P5558_14880"
     CDS             5146..7005
                     /locus_tag="P5558_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY25649.1"
                     /translation="MTAAPPARPVPAATPQADRRRRLPLSWFLAAVIGLAMLVAMALL
                     LWLTLGTARDNTLQLLRDKAQLVLTLVRARTNQFLEPAEALAGDVARRIERGEINTAD
                     QSALVQALGYALSAAPQINAAVYVDAEGWLVSVFRDRETGRLGSSFEDWQADAAVKAA
                     VAQLHLDSEPQPHWGEPLFSAGAAATLVYFAHPVRERDGDLGIVVATTTVQDLSTFIG
                     SIDTPGTIGTFILYGRDKVLAHPRLRVASELMTADAPLPSLAEIGDPALAAIWAPGWE
                     ERRIADLDIPAHGSRDDSDSYVYLYDELTTARDTPWVIGGYFRAEDLGREWRRLNVVT
                     GIAAALLVLSLGGAVLIARLLAGPATQIADTARAISALQLDRIEPLAGSRIRELDDAE
                     HSLNAMVAALRSFVRYLPSDLVHYVLRHPERDIGKPQLRSMTILFTDISGFTTLAENL
                     EPAAVGALLNAHFADLEACIRGTGGTIDKYLGDGLLAFWGAPEPLADHQRRAVEAALA
                     MAEATSRRNAEVALPLRLRIGIAAGEILVGDLGAPTRMNYTVIGDSVNLAQRLLEIGH
                     SAAPAHETVIVTTAECIAAIEPPPRTRPLGQHVIRGRAGAVELVEVLPPTTLP"
     gene            7002..7952
                     /locus_tag="P5558_14885"
     CDS             7002..7952
                     /locus_tag="P5558_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25650.1"
                     /translation="MTPAASALGWIGRQSSPLFFVAVFAGVLLPPLARLLQPLLLPAI
                     LLPFVVALVRLDLAALGRQARRPWLALGLVLWSLVGCPFLVALVLQSLPLDPDIAALL
                     VTTAACAPLMASGALALLLGLDVALALLVVVPATALVPFTVPPLALGLAGIAIDLPAA
                     ALALRLALMVLGSAALAALVRRWLGPERLVTARPGLDGLAVLGFVVFAVGVMDGFTMT
                     ALARPALVGGIVVAVFALNLGLQAITVLALLPFLPLRTALTAGLVAGNNNLGLILAAV
                     IDTAPPAMLIFVATAQFPIYLLPIVQRPLYRRWLASEPPA"
     gene            7949..8488
                     /locus_tag="P5558_14890"
     CDS             7949..8488
                     /locus_tag="P5558_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRY25651.1"
                     /translation="MTPREVLDFWFDPANQPFWFKKSAAFDEAVRHRLGDAHAAAAAG
                     RLDDWRATPEGALALVILLDQVPRNIHRGTARAFATDDAALAVAAAAIDDGFGRGLDK
                     DQRLFLYLPLEHSEKLADQERSVALFTELGDARLTDYAVRHRDIVARFGRFPHRNALL
                     GRASTEEEISFLEGPNSSF"
     gene            complement(8501..9181)
                     /locus_tag="P5558_14895"
     CDS             complement(8501..9181)
                     /locus_tag="P5558_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25652.1"
                     /translation="MATVGEELGLPRAADYPYAAPLERFVFAADGEPPAFAVDGRTPV
                     LALGSNAAPAQLRRKFAGHTGHIPVSRAVLLDHVVVYSAHFSRYGALPATLHRHPGAV
                     AFVAVTWLDPEQLARMHQTEAIGVNYDYVESTDLRLEHDDDDMAAPALVGAYVSRAGA
                     LLHQGSPIRLAEVATSGCPWPALIQPAVMRFAHKRVAPELAVEEFLARLVQSETFRRS
                     CAERLRLG"
     gene            complement(9299..10189)
                     /locus_tag="P5558_14900"
     CDS             complement(9299..10189)
                     /locus_tag="P5558_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRY25653.1"
                     /translation="MAETAVEGRGAERAGKPALHRRLGRGIALLGRAGKRMSDEDGFV
                     MAGHLSFTALLALFPFLIFLTSLASFLGTQADLERIVALMFDSMPGEVAETLEPAVAE
                     VVVGRRAGFLGFSILMMLWAASSGIEAIRLAFNRAYRVSRFRPFWMRRLQTFFFVMLA
                     ILLVPAMSVLVVFGPILWNLLTWWIDLGFAYRLVWTVARNVVAIAILFVTLVVLHRWL
                     PNRRHHVRDILPGVVFTVLVLMVAGTGFSTYLASFASYSVTYGSMASVVIALIFFYIT
                     SIILVLGAYVNAAIMGIGRR"
     gene            10354..11337
                     /locus_tag="P5558_14905"
     CDS             10354..11337
                     /locus_tag="P5558_14905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HRY25654.1"
                     /translation="MARALWLRKTETGQTVEIGPITEADLPDGEVLVDVACSTLNFKD
                     ALAITGKAPIVRSWPMVPGIDFAGTVKASRHAAWQPGDSVILNGWGTGETRLGGLAEI
                     ARVPGDWLVPLPEGYSLADAMAIGTAGYTAMLAVLRLEAAGIEPGSGPLLVTGAMGGV
                     GSVAILLLAQAGFEVWALTGRPAEAEHLKNLGAAGIVPREELASPGKPLVRERWAGGI
                     DAAGSHVLANLLAATKYGGTVAACGLAAGMDLSMTVAPFILRGVTLAGIDSVYVPNLQ
                     RRQAWHRLARDLPAGRLREISRSIPLDEVVAAAAELLAGRVRGRLVVTLGN"
     gene            11485..11694
                     /locus_tag="P5558_14910"
     CDS             11485..11694
                     /locus_tag="P5558_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRY25655.1"
                     /translation="MPVGTVKWFNSTKGYGFIQPESGGADVFVHISAVERAGMSSLNE
                     GQRVSYEEQLDPRRGKKSAENLKPA"
     gene            complement(11785..>12193)
                     /locus_tag="P5558_14915"
     CDS             complement(11785..>12193)
                     /locus_tag="P5558_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY25656.1"
                     /translation="IKVTAKEYGILELLALRRGTTLSKETFLDHLYGGRDEPEQKIID
                     VFICKLRKKIAAVTGDARGIETVWGQGYVLREPAPGMPGLAEQAPAFFGPGLTSKALH
                     ELRAVELDELLLQLLPRTAGLAMAGLGMAHTQP"
BASE COUNT         1896 a         4208 c         4258 g         1831 t
ORIGIN      
        1 cggttctcga cgtggacatg gccggggaac gatcgatgcc catcgtggcg gcactcaagg
       61 cacggggggt gcccttcgtg ctcgtcacgg gctactccgc gatgcagcta cccgaagcgg
      121 agttcgaggg ggccacgcgc atcggcaagc cctatggcga cggcgagctg gtcagggcga
      181 tcagggccat gctcggctcg cctactccgc cgcgatgagg tgccggtcgg tcgaggcgag
      241 cccgcgcggg tccttgtaga ggcggccggc ctgcatgatc atggcgagat tggcctggtg
      301 ctggacgagg gacacgtcct cgagcgggtt gcccctgacc aggaggagat cggcgatgaa
      361 gccttccttc acccggccga gctcagcgcc catgcccatg agctcggcgc cgacgacggt
      421 ggcgcagcgc agcgcctcgg cgggcgcgta gccgaaatac tccacgaagt gctcgatgtc
      481 gcgggcgttc tggcccatcg gcgtcaccgc gaagccgtag tcgccgccga tgacgacgcg
      541 gatgccgcgc ttcctgatct cgtgataggt ggcgcagcaa gcgtcaaact tccggtagag
      601 gtcgagctgc cggcagacct cgggggagag gccgatgccc tcgccctcct tcgtgcagtt
      661 gtggacgagg ccgaaggccg ggccgacgaa ggccgtgtcc ttgacgctct caaggaggtc
      721 gagcgcctcc tcgtcggcga agtcgcagtg gtagatgcag tcgacgccgt ggcggaccgc
      781 catcttcacg ctctccgacg agcgggcgtg ggccgcgacc ttcttgccga agccgtgcgc
      841 cacctccatc acggtggcga gctcggcgtc ggtcagcggc gtgatctcgg cccgggcgtg
      901 gctgacgaac tcgtcgcccg agatgttgat cttgatggtg tcgacgccgt cgcggatgca
      961 ttcccgcacc acccggcgca tctcgtccgg cccgtcggcg acgaggccga agctctcctg
     1021 gtggatgtgc cgcttgcgct cgtcgccgag cccggcggtg accgtgatct ccgggctgcc
     1081 ggcgcgcatg cggggcccgg cgagcaggcc ggaattgatg gcgttccggg ccgtgacgcc
     1141 gagcaggggc ttggccgagg cggcctcgaa gatgctggtg aagccgtggt cgagcagcag
     1201 ccggcagttc ttcgccgtga gcagcatgtg ctcctccggc gggatcaggc cgagctcgaa
     1261 gggggcgttg atgccggtga agctcgggtg gcagtggccc tcgaccatgc cgggcatcag
     1321 ggtcatgccg ctgccgtcga tcacggtggc accctcggcg ccgatcgagg cgccatccct
     1381 ggcgatgcgg gcgatgcgct cgcccgcgat ctcgacctcg gccttgaagg tgctcttgct
     1441 ctcgccgtcc cagacggcgg cgtgcctgat gacggcgcgc tccatgatcg gcctccctga
     1501 attggctctg cgggggagct tagcgcaggc gcggcacgca ggggaacggc taacgcgcgg
     1561 gcgggggatc gcccaccgtt cactggtacg cagtcgttcc ggccaaggta tcggctgcca
     1621 gttcgaaagc gcccagtcca agttctattg tcgctccgtc agcagcagtg gggtagcacc
     1681 ccttcctcag tctgccggcc gtagctcaac gagcaaagcc gatgcgaggc gtgcgcaggc
     1741 ggcgctctca gtcggcgaca gaagaacgaa cccaaagatt ggcaccacga aatggccctc
     1801 gatcccggca gcgcgcgccg cgccgccgac gcgaaagcat gccgtctcga gttcaaccgc
     1861 ggtgacggcg atccggtcga ccacttcatt gaatgcggcc aaactctggc ttctccaggg
     1921 gtcccttttc gcttcgactc catggcggac gaagagaatg tagcgcagac cgagttcccg
     1981 cagcttggca gccacacgcg ggcgcgccag gaactcggac gccgccgctt cgctcactgg
     2041 tatggattgc gcggtctcga gccaggggaa gaatgcgtcc cgaacggcag agtcgtcgag
     2101 gacgcgggct ttggacagga cctccacgag atacccagta aggcaatcgg tgctccagcc
     2161 actcgccatg cggtcatcgg ccaggacagc gagcgtctca ccgggccgga gcggctcgat
     2221 ctcggcggcc ggaggcacct ccagtgtcga aatcggcggc gacggaaccg gccacgccgt
     2281 gcaggaggag aggcaagaga cagcgaccag aaagaacatg cgcatgtcag gtcattgcct
     2341 cccgacgtgc cgtcagccaa gcgacgatag ccggctggac cgcgaacgag cggaccagct
     2401 cggcggtcgc gccgcgcagc gtctgcgcca atgcctcctc tgaaccgtca tacacagtcg
     2461 cgcccgagat cttccaggtc ccgatatcgg tgccgtcggg atcaacaaag gtgatgccgt
     2521 agtctacgga ccacgcgacc acgtagttgc gtaaagacca cgcgtccttg gttacctcga
     2581 ccccaagaag cgcaatggtc agcgagccat tatcgccggg cctcgggggc cccgcacggg
     2641 ccgactcgac gagcgccggc agtgtccgct cgaagatcgc ccgcgtcgcc ggcccaagcc
     2701 gcaggtgagt tgttagcgtg ccgctcgaaa cggcactgaa ggtcacctcc tggtcgacgc
     2761 tcggctcgaa gacgtagctc aaccggagcg gcaacggctc gatcagaggc ggtagccagc
     2821 ccgttccagg cgcgggctcc agcaccacgt ccttcccgcc accgcagccc acgactgcaa
     2881 tagccggcaa gaccaggaga gcagcgaggc gacgacggcg catgcccagc caaccttccc
     2941 catcccgcga aggccgagcg ataccattct cggcgcaatc tgtacttgcg ctagatcaag
     3001 ccgtcaggcg gcgccaaccg cggctcagtg tacagtgtcg cccgggcggg cgaagcgggc
     3061 gtcgtcgggg tagtggtcga agcggacggt ctgcgtcagg aaagccgcct cgtcgcgcca
     3121 gccggccatg agcggccagc cgttgcgggc gtagacggtg aacggaatgg cgccgcagtt
     3181 ctcgcgctgc accgggcgga gcgccggggc gacgcgctgc atcagctcga gcgagcggac
     3241 cacgggctcg aggaacggcg tcatccaggc gctggccagg acctcgccgc agatctgctc
     3301 gtcgatctgc agcacatgat aggtgctggc atggccggcg accggcggcc ccgcactcca
     3361 gtgatcgagg acgtaaggca gggccgaggc gccgtcatcg gcgagcccgg cccggatcag
     3421 ggtcggcgcg ccgccgtcga gccggtagac attctgcggt gccaggtcga cgaggtagtg
     3481 ctcgccggcg acggtgacga gcacgcggcc gtggtcgctg ccggtctcga ggcgcaacgg
     3541 aacgtcgtcg aagagcgcct cgaccagcat cccggcccgt gctgccggca ccgggccgag
     3601 gcccgccagt agaagcaggc cggccaggga ccgccgccat gttcgcatct tttgctccag
     3661 ataggcttgc ccgagcatgc cggatcgggg tgaacggaat gttaagatac cgccaaggca
     3721 cgctcgagca gcatcggcac atggcgggtg tggcgggcgg ggtcgaagag gtcggccagg
     3781 ctcttctcgt cgagcacggc ggtgatcctc ggatcgacct cgagcaggtc gaggagcggc
     3841 tcctcgttct cccaggcacg catggcgctc gcctggacca gctcgtaggc cgcctgccgc
     3901 cccatgccgg catcgaccag tgccagcagc accgtctggc tgttgaagag gccgtgggtg
     3961 gcatcgaggt tgcggcgcat gcgctcgggg tggacgacca gccggtccat gaggccggcg
     4021 agccgggcca gcgcgaaatc ggcgaggatg caggaatccg gcagcatgac gcgctcgacc
     4081 gagctgtgcg agatgtcgcg ctcgtgccag agcgccacgt tctcgagcgc cggcacgacg
     4141 gcggcgcgga tcatccgggc gaggccggtg aggttctccg acagcaccgg gttgcgcttg
     4201 tgcggcatgg ccgagctgcc cttctgcccg gcgtggaaga actcctcgac ctcgcgcacc
     4261 tcgctgcgct ggaggtggcg gatctcggtc gcgaggtgct cgatcgagcc cgcgatctgg
     4321 gcgagcgcac aggcgaaggc ggcgtgccgg tcgcgcggga tgacctgggt ggagacgggc
     4381 tcgacccgca ggccgagctt gccggcgacc ctcgcctcga tcgcggggtc gatattggcg
     4441 aaggtgccga ccgcgccgga gatggcgcag gtcgagacgt cctcgatggc ggcgagcaac
     4501 cgctggcgct gccttgcgaa ggcggcatgg aagccagcga gcttgaggcc gaaggtggtg
     4561 ggctcggcat ggatgccgtg gctgcggccg atgcagacgt ccatggcatg ctcgcgtgcc
     4621 cggcgggcca gcacggcgag gagggcgtcg agatcggcga ggacgagctg gccagcctcg
     4681 cggagctgca gggcgagcgt cgtgtcgagg aggtcggacg aggtcatgcc gagatgcagg
     4741 agccgggcat cagcgccgca cagattctcg acatgggtca ggaacgcgat gacatcgtgg
     4801 cgggtggtgg cctcgatcgc ctcgaccgcc gccgcgtcga tcagccgatc ccgggcctgc
     4861 tccgtcgccg tgcgcaggcg ggccaccgcc tcgccatcga tctggcccag gccggccatg
     4921 gcctcggcga ccaggagctc gatctcgagc cagagctcaa ggcggcgctc cggggcgaag
     4981 acggcgacca tctgcggccg gctgtaacgg gggatcatcc gggactccag gcgggggcgg
     5041 gcattctctt agcagcgacc ggaccgaacc ggaagatcgc cgccggaggc aaggtgacga
     5101 tccgccttgg ccaagtggcc acgccagcat tacaaaccag cgatcatgac cgccgcacca
     5161 cccgcccgcc cggttcccgc cgcgactccg caagcggacc ggcggcgccg cctgccgctg
     5221 agctggtttc tggcggcggt catcggcctc gccatgctgg tcgccatggc gctgctgctc
     5281 tggctcaccc tcggcaccgc gcgcgacaac acgctgcagc tcctgcggga caaggcgcag
     5341 ctcgtgctga cgctggtgcg ggcgcgcacc aaccagttcc tcgagccggc ggaagcgctg
     5401 gccggcgatg tcgcccggcg catcgagcgc ggcgagatca acaccgcgga ccagtcggcg
     5461 ctggtccagg cgctcggcta tgcgctgtcc gccgcgccac agatcaatgc ggccgtctat
     5521 gtcgacgcgg agggctggct cgtcagcgtc ttccgcgacc gcgagaccgg ccgcctcggg
     5581 tcgtccttcg aggactggca ggcggacgcg gcggtcaagg cggcggtcgc ccagctgcat
     5641 ctcgacagcg agccgcagcc gcactggggt gagcctttgt tcagcgccgg cgccgccgcg
     5701 acgctggtct atttcgccca tccggtgcgc gagcgcgacg gcgacctcgg catcgtcgtc
     5761 gccacgacca cggtccagga tctctcgacc ttcatcggct cgatcgacac gccgggcacg
     5821 atcggcacct tcatcctcta cgggcgcgac aaggtgctgg cccacccgcg gctgcgcgtc
     5881 gccagcgagc tgatgaccgc ggacgcgccg ctgccgagcc tcgccgagat cggcgatccc
     5941 gctttggcgg caatctgggc acccggctgg gaggagcggc gcatcgccga tctggatatc
     6001 ccggcccatg ggtcgaggga cgattccgac agctacgtct acctctacga cgagctcacc
     6061 accgcccgcg acacaccttg ggtgatcggc ggctatttcc gcgccgagga cctcggccgg
     6121 gaatggcggc ggttgaatgt ggtcacgggc atagccgcgg cactcctcgt gctcagcctc
     6181 ggcggggcgg tcctgatcgc ccgcctgctg gccgggccgg cgacgcagat cgccgacacg
     6241 gcgcgggcga tcagcgcgct tcagctcgat cggatcgagc ccctcgccgg ctcgcgcatc
     6301 cgcgagctcg acgatgcgga gcactcgctc aacgctatgg tcgcggccct ccgctccttc
     6361 gtgcgctacc tgccgagcga cctcgtgcac tatgtgctgc gccacccgga gcgggatatc
     6421 ggcaagccgc agctcaggtc gatgaccatc ctcttcaccg acatctcggg cttcaccaca
     6481 ctggcggaga acctggagcc ggcggccgtg ggggctctgc tcaatgccca tttcgccgat
     6541 ctcgaggcgt gcatccgcgg cacgggaggc accatcgaca agtatctggg cgacggactg
     6601 ctggccttct ggggcgcacc ggagccgctc gccgaccatc agcggcgggc ggtcgaggca
     6661 gcgctcgcca tggccgaggc caccagtcgg cgcaacgccg aggtggcgct gccgctgcgc
     6721 ctgcggatcg gcatcgccgc gggcgagatc ctggtcggcg atctgggtgc tccgacccgg
     6781 atgaactaca cggtgatcgg cgattcggtg aacctcgccc agcgcctgct cgagatcggg
     6841 cacagcgccg ccccggcgca cgagacggtg atcgtgacca ccgccgaatg catcgccgcg
     6901 atcgagccgc cgcccaggac ccggccactc ggccagcacg tcattcgcgg ccgggccggc
     6961 gccgtcgagc tggtcgaggt gttgccgccg accaccctgc cgtgacgccg gccgcctcgg
     7021 ccctcggctg gatcggccgc cagtccagcc cgctcttctt cgtcgccgtc ttcgccgggg
     7081 tgctgctgcc acctttggcc cggctgctgc agccgctgct gctgccggcg atcctcctgc
     7141 ccttcgtcgt ggccctggtg cggctcgatc tcgcagcgct cggccgccag gcgaggcgcc
     7201 cgtggctcgc gctcggcctc gtgctgtggt cgctggtcgg ctgccccttc ctcgttgccc
     7261 tggtgctcca atccctgccg ctcgacccgg atatcgcggc cctcctcgtg acgaccgccg
     7321 cctgcgcacc gctcatggcg tccggagcgc tggcgctgct gctcggcctc gacgtggcac
     7381 tggcgctcct cgtcgtcgtg ccggcgaccg cgctcgtgcc gttcaccgtg ccgcccctgg
     7441 cgctgggcct cgccggcatc gccatcgatc tcccggcagc agccctcgcc ttgcgtctgg
     7501 cgctcatggt gctgggctcg gcggcgctcg ccgcgctggt gcgccgatgg ctcgggcccg
     7561 aacggctcgt gactgcccgg ccgggtctcg acgggctcgc cgtcctcggc ttcgtcgtct
     7621 tcgccgtcgg cgtgatggac gggttcacga tgaccgccct cgcccgcccg gccctggtcg
     7681 gcggcatcgt cgtcgccgta ttcgcgctca atctcggcct gcaggcgatc accgtgctgg
     7741 ccctgctgcc gttcctgccg ctgcgcacgg cgctgacggc cgggctggtc gccggcaaca
     7801 acaatctcgg gctgatcctg gcggcggtga tcgacaccgc cccgccggcg atgctcatct
     7861 tcgtggcgac cgcccagttt ccgatctatc tcctgcccat cgtccagcgg ccgctctatc
     7921 gccgctggct cgcttcggag ccgcccgcat gacgccgaga gaagtgctcg acttctggtt
     7981 cgatccagcc aaccagcctt tctggttcaa gaagagcgcg gccttcgacg aggccgtgcg
     8041 gcaccggctc ggcgatgccc atgcggcagc cgcggcgggc cggctggacg actggcgggc
     8101 gacgcccgag ggggcgctgg cgctggtcat cctgctcgac caggtgccgc gcaacattca
     8161 tcggggcact gcccgggcct tcgccaccga tgatgcggcg ctggcggttg ctgccgcggc
     8221 catcgacgac ggcttcggcc gggggctcga caaggaccag cgtctcttcc tctatctgcc
     8281 gctcgagcat tctgaaaagc tcgcggacca ggagcgatcg gtggcgctgt tcacggagct
     8341 cggcgatgcg cggctgaccg actacgcggt ccggcatcgc gacatcgtcg cccgcttcgg
     8401 ccgctttccg catcgcaacg ccctgctcgg ccgcgcctcg acggaggagg agatcagctt
     8461 cctcgagggg ccgaactcgt ccttctgacg ccctgccccg tcagccgaga cgcagccgct
     8521 ccgcgcagga ccggcggaac gtctcgctct gcacgagccg ggcgaggaac tcctcgaccg
     8581 ccagctcggg cgccacgcgc ttgtgggcga agcgcatgac cgccggctgg atgagcgccg
     8641 gccaggggca gcccgacgtc gcgacctcgg cgagccggat cggcgaaccc tgatgcagca
     8701 gggcaccggc ccggctgaca taggcgccga cgagcgcggg tgcggccatg tcgtcgtcgt
     8761 cgtgctcgag ccgcaggtcc gtggattcga catagtcgta attgacgccg atcgcctcgg
     8821 tctggtgcat ccgggcgagc tgctccgggt cgagccaggt cacggcgacg aaggcgaccg
     8881 cccccggatg ccggtgcagg gtcgccggca gggcgccgta gcggctgaaa tgcgccgagt
     8941 agacgaccac atggtcgagc agcacggccc ggctgaccgg gatatggccg gtgtggccgg
     9001 cgaacttgcg acggagctgg gccggcgccg cgttcgagcc gagggccagg acgggcgtgc
     9061 ggccgtccac ggcgaaggcc ggcggctcgc cgtcggccgc gaagacgaag cgctcgagcg
     9121 gcgctgcgta gggatagtcg gccgcgcgcg gcagcccgag ctcctcgccg accgtcgcca
     9181 tgatcccgct ccagcaacat cacctcgccc ggcaggcgcc cgatcgacct cctgccgacg
     9241 tcggttctac tcagagtgta acccaccgcg ccgagccggg caatcgacgg gatcaacctc
     9301 acctgcgccc gatccccatg atcgcggcat tcacataggc cccgagcacc aggatgatcg
     9361 atgtgatgta aaagaagatc agggcgatca cgacgctagc catgctgcca tacgtgacac
     9421 tgtagctcgc aaagctggcg agataggtcg agaagccggt gcccgccacc ataagcacga
     9481 gcacggtgaa gacgacgccc ggcaggatgt cgcgcacatg gtgccgccgg ttcggcagcc
     9541 agcggtgcag gacgacgagg gtgacgaaca ggatggcgat cgccaccacg ttgcgggcga
     9601 ccgtccatac cagtcggtag gcgaagccga ggtcgatcca ccaagtcagc aggttccaca
     9661 ggatcggccc gaagaccacc agcaccgaca tcgccggcac caggagaatt gccagcatga
     9721 cgaagaagaa ggtctgcagc cggcgcatcc agaacggcct gaagcgcgac acgcgatagg
     9781 cgcggttgaa cgcgaggcgg atagcctcga tgccactcga ggcggcccag agcatcatca
     9841 ggatactgaa tccgagaaac ccggcgcgcc gcccgaccac gacctcggcc acggccggct
     9901 cgagcgtctc cgccacctcg ccgggcatgc tgtcgaacat gagggcgacg atgcgctcga
     9961 ggtcggcctg cgtgccgagg aagctggcga gcgaggtgag aaagatcagg aacgggaaga
    10021 gcgccaaaag cgccgtgaac gaaaggtggc cggccatgac gaagccgtcc tcgtccgaca
    10081 tccgcttgcc cgcccggccg agcagcgcga tcccgcggcc cagtcgtcgg tgcagagcgg
    10141 gcttgccggc ccgttccgct cccctgccct cgaccgccgt ttccgccatg cgaagctttc
    10201 catcaccgtc tggatcgccg gcagattggc ccgcacctcg ccggtctggc aagcggcgcc
    10261 aagccggcac ggggcaggcc aggctgacag gccgccaagc tccggctacg ctctccctca
    10321 cattgttcgc atcaaacggc gaaaggaaac gccatggccc gagccctctg gctgcgcaag
    10381 accgaaacgg ggcagaccgt cgagattggc ccgatcaccg aggccgacct gcccgatggc
    10441 gaggtcctgg tcgacgtcgc ctgctcgacc ctgaacttca aggacgcgct ggcgatcacc
    10501 ggcaaggcgc cgatcgtccg atcctggcca atggtgccgg gcatcgattt cgccggcacg
    10561 gtcaaggcga gccggcacgc cgcctggcag ccgggcgact ccgtgatcct caatggctgg
    10621 ggcaccggcg agacgcggct cggtgggctc gccgagatcg cccgggtgcc cggcgactgg
    10681 ctggtgccgc tgcccgaggg ctacagcctc gccgatgcca tggcgatcgg cacggcaggc
    10741 tacacggcga tgctcgccgt gctgcgcctc gaggcagcgg gcatcgagcc gggttcgggc
    10801 ccgctgctgg tcaccggggc aatgggcggg gtcggcagcg ttgccatcct gctgctggca
    10861 caggcgggct tcgaggtctg ggcgctgacc gggcggccgg ccgaggcgga gcacctgaag
    10921 aatctgggtg ctgccggcat cgtgccgcgc gaggagctcg cttcgcccgg caagccgctc
    10981 gtccgggagc ggtgggccgg cggcatcgat gcggccggca gccacgtcct ggccaacctg
    11041 ctggccgcca cgaaatatgg tgggaccgtc gccgcctgcg gtctcgccgc cggcatggac
    11101 ctgtcgatga cggttgcccc gttcatcctg cgcggcgtga cgctcgccgg catcgacagc
    11161 gtctacgtgc cgaatctaca gcgccggcag gcctggcacc ggcttgcccg cgacctgccg
    11221 gcgggtcggc tcagggagat cagccgcagc attccgctcg acgaggtcgt cgccgcggcg
    11281 gcggaattgc tggctggtcg ggtgcgcggc cggctggtcg tgacgctcgg caactgaccc
    11341 cacggggatg tctcgccaca cacgaatcaa agcccgcttg cttcatgacg ggaatggcat
    11401 tcataatgca tggcgagcaa ggttgctcac tgggcaaagg gccgggggtc gaatcggcgc
    11461 taccagcacg atcggaggac aaggatgcca gtcggcaccg tcaagtggtt caacagcacc
    11521 aagggttacg gcttcattca gccggaaagt ggcggtgcgg acgtcttcgt tcacatctcg
    11581 gccgtcgagc gggccgggat gagcagcctg aacgaaggcc agcgggtctc ctacgaggag
    11641 cagctcgatc cgcggcgcgg caagaagtcg gccgagaacc tgaagccggc ctgaccggcg
    11701 cggcaacggc ggtatgatac gaaggcaccg ggcgaccggt gccttctttt ctgtgtgcca
    11761 ccggtcgcgc ctcgcatcgc ggcattacgg ttgagtgtgc gccatgccca gcccggccat
    11821 ggccagcccc gccgtgcgcg gcaacagctg cagcagcagc tcgtcgagct cgaccgcgcg
    11881 gagctcgtgc agcgccttgc tggtgagccc agggccgaag aaggccgggg cctgctcggc
    11941 gaggccgggc atgcccgggg ccggctcacg cagcacatag ccctgccccc acaccgtctc
    12001 gatgccgcgg gcgtcgccgg tgaccgcggc gatcttcttc ctcagcttgc agatgaagac
    12061 gtcgatgatc ttctgctcgg gctcgtcccg tccgccatag aggtggtcga ggaaggtctc
    12121 cttgctcagc gtggtgccgc ggcgcagtgc caggagctcg agaatgccgt attccttggc
    12181 ggtcaccttg atc
//
LOCUS       DAOTFI010000145        12188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6220_length_12188_cov_8.072529, whole genome shotgun sequence.
ACCESSION   DAOTFI010000145 DAOTFI010000000
VERSION     DAOTFI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6220_length_12188_cov_8.072529"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..761)
                     /locus_tag="P5558_14920"
     CDS             complement(51..761)
                     /locus_tag="P5558_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY25657.1"
                     /translation="MDRQQTHCCGARVDCANCGIRPIAICGALAPIALGKLAAIAGSR
                     RVDAGAQLIQEGEHAQDIFTLTEGMLKIYKLLPDGRRQITGFMIPGDLVGLAYGDSYI
                     YSAEAVTDIVACRFRRGSLLALMADHPELEHRLLSRAGNELAAAQAQMLLLGRKTARE
                     RVASFLLGLAGRLDLGQGEPMPLPMNRGDIADFLGLTIETVSRTLTLMRKAGLIILPD
                     KHSVTITDLASLRAASGD"
     gene            900..1418
                     /locus_tag="P5558_14925"
     CDS             900..1418
                     /locus_tag="P5558_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25658.1"
                     /translation="MMIALSPVASSYMLPIAGLPWYWRDLEHDRIDEVLPLVLAVDPD
                     RTEAPHWRRDAQDRIGNASAGSGIVIAQCFAGLTLAYFFHVCSRAPDGRPRLVVDRLR
                     WMELGRPHRSLDALLAIVLQTATRFACAEVLLEERACVERRARAALEQRASETGFTAT
                     AGAWRRVVPSSR"
     gene            1650..3182
                     /locus_tag="P5558_14930"
     CDS             1650..3182
                     /locus_tag="P5558_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25659.1"
                     /translation="MFYTPKNALFRRVGRRKFLELGGGAATALGAGSIAVGLNSVVTR
                     RPAYAQSSEEAKWRQFEGTKLVFMSENTPPSFAIRENLQPFYDLTGIEVEILTDDLPV
                     VQQKVGIDLRGGSADFHLNYVQDKPIGAPFADFYVDLTKFMADDTLPQDPDGSGEDAW
                     FENFLTACGVYYDEGRIVAFPYDCAVACTFYRQDLFEQLSKDFEAEHGYRMEFNADTT
                     WQNVQDFAAFFKKLREGGREDIPYGYAQHQGSFAWTTQLDIQRLLFANGRWTDFEGVD
                     DKLGSREPGPTRWGDEQSVLIMQKFKDQADVSHPDNLANGTLELNTVYQAGQIAMQVQ
                     YHEFAASIEDESTSRAAGGKTGYGVCPLGAKEWIVNGGEAVNGTNCGIGGIGINAGAS
                     EELQRAAYLFAIWSTSKNTQFSVLKGVGGTPTRKAVLEIPEVQAARQRPTNMPNALTF
                     EPVYDYGIRDPHFVLGPKIPEANEYHKILATEVQRCISGQTSAEEACEEIRWQLDDMH
                     GH"
     gene            3280..4293
                     /locus_tag="P5558_14935"
     CDS             3280..4293
                     /locus_tag="P5558_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25660.1"
                     /translation="MAVTTAGGGRGIGGGASAAAGKQHLLEMREGARVISHTPARLNT
                     APSRAYYFYLMLPAIIVLACITLYPFFWLVWMSLHDVDVGAAADKWNDFANWIRLGRD
                     RKFQDGWWLLLQYSAMCMVLQIGLGVLLAVILNGSRHEKLLVTIFLMPMMMAPVVAGL
                     VWYYLYNSTFGWYHWLMQSAGILGERSILGDPSTAMLGIVIVDTWQWTPLITLIVLAG
                     LKRVPRDQLEANMVDGAGEIRNFFQITLPNIYPFLLIAILLRFMDNFRFIDHILVLTG
                     GGPADSTKILPTYLFDVSFQFFKLGRGAAIALTLLAVTIVLGLILVRVFQSPTAQTRP
                     EDS"
     gene            4293..5171
                     /locus_tag="P5558_14940"
     CDS             4293..5171
                     /locus_tag="P5558_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25661.1"
                     /translation="MASSDVVRYRSPLRSLVNVLVAIFLVIYVVWTVLPLFVMFVSSF
                     KDLLEAFKLPTVGDWSGITLFFDFTPTVKHYQSLFLDHNFSVYLFNSIVASAGSALIS
                     VVLGAMCAYSLSRINFRGKQDLFFWIISTRMAPVVAVMVPLYAIFRGAGLVGTIPGLI
                     LAYTTFNLPFAIWMLKGFFDNVPYAIEEAQMCDGATRFRAFLSILPLVAPGVGAFLVL
                     CILFAWNDFLFASIIGSGGAKTLPVATKELVQPQNIQWGSIMAAGVVTTLPMMFLGLL
                     IRRYLVAGLTMGAVKE"
     gene            5179..6270
                     /locus_tag="P5558_14945"
     CDS             5179..6270
                     /locus_tag="P5558_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25662.1"
                     /translation="MASVTIDHVWKYYGKTEAVKDLNLKCADGEFLSVLGPSGCGKSS
                     TLRMLAGLEHISAGDIRFGERRINDLPPKDRDIAMVFENYALYPHKTVRENIANSLML
                     RGASRATIEQKVKKATELLQIGHLLQRKPQELSGGQKQRVAIGRAIVREPQLFLFDEP
                     IAHLDAKLRAHMRGELKIMQRELGTTMIYVTHDQLEALSMADRIAVMHEGVLQQFGTP
                     QEIYDHPVNEWVAGFVGEPPMNFVDCLIEDRGGVLHAVHPSFAVPVTGSQEAILRDSA
                     HRAEVRMGIRPDQIGIVTGDGGDAVAAQILLTEPLGGDMLVDAAVGQEKFLVKTKPDF
                     QGRRGDACRLAFDTGRWHLFDRDTGLAYF"
     gene            6329..7405
                     /locus_tag="P5558_14950"
     CDS             6329..7405
                     /locus_tag="P5558_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HRY25663.1"
                     /translation="MQALRFHAAKDLRLDDIAAPPARPADDEVVIRNRFVGICGTDLH
                     EYSYGPIFIPTEPHPFSHAKAPQVLGHEFGGVVEAVGAGVTSVAPGDRVSVQPLLMPR
                     TGEHYADRGLFHLSGSLALVGLSWGWGGMAELAAVKEYNVFKVPDALTDEEAALVEPT
                     AVAVYACDRGRVAAGNSVLVTGAGPIGALVLLAARAAGAAPLFVSDVNDARLDFVRAM
                     LPDVVTINPSREKPGDVVRAATDGRAGVDVAIECVGNEHALKDCLDAVRKQGVIVQTG
                     LHPHENPLDWFQVTFKDIDIRGSWCYPTYLWPRVMRLIASGLLPAKKIVTKRIGLGEA
                     VPAGFEALLDPGGTQVKILIDLSK"
     gene            7577..8545
                     /locus_tag="P5558_14955"
     CDS             7577..8545
                     /locus_tag="P5558_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRY25664.1"
                     /translation="MQLSRDDLLEAYRKMRMIREFEDAVHEEFSAGNIPGFVHLYAGE
                     EASGVGVCMHLDDRDAIASTHRGHGHCIAKNCDERAMMFEIFGRKEGLCGGKGGSMHI
                     ADMSKGMMGANGIVGGGPPLICGAALSAKYLKTGGVAIAFVGDGASNQGTTFESYNLA
                     KVWNLPAIFVVEDNGYAESTASKWSVAGSQVDRAKGFGMPSYDVDGHDFFAVWEAARE
                     AIERARNDGGPSLIHSRLNRYYGHFEGDAISYKAPGEIEKVKAEKDAIMLFRNRVSEA
                     GLLDGPAMDAVDKACRERIAQVLAEAKAGAMPTEADLLTDVYTSAY"
     gene            8558..9571
                     /locus_tag="P5558_14960"
     CDS             8558..9571
                     /locus_tag="P5558_14960"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRY25665.1"
                     /translation="MPKKTYRQAINEALRQEMERDHRVILMGEDISGGMGAPGEQDAW
                     GGPLGVTKGIMPVFTRDRVLDTPITESAFIGAAAGAAATGLRPVAELMFVDFMGVCFD
                     QIFNQAAKFKYMFGGKAVTPLTIRTMYGAGFRAASQHSQCLYPLFTHIPGLKVALPSN
                     AYEAKGLLATAIRDDDPVMFFEHKAMYDDEAEVPDEAYTIPFGEANVTREGDDVTIIA
                     FGRMVNFANEAADNLEKSGISCTVVDPRTTSPLDRDTILETCEETGRVVVVDEAHPRC
                     NMATDIAAMCAQEIFGALKAPVKMVTGYHCPVPFSPALEDLYVPNAAKIEAAVREVAG
                     KGH"
     gene            9660..10772
                     /locus_tag="P5558_14965"
     CDS             9660..10772
                     /locus_tag="P5558_14965"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin dehydrogenase
                     dihydrolipoyllysine-residue acetyltransferase subunit"
                     /protein_id="HRY25666.1"
                     /translation="MSDAIQMIVMPKWGLAMQEGMVAQWHVAEGAKITKGQEIVDIET
                     SKIANVFESPISGTLRRLVVKDGETVPVAYLIGVVAEESVTDAEIDAFVEDFKANFVF
                     DAAGAGGPEPEDATTAAGRIRYLEMGDGEGAPIVFIHGYGGDLNNWMFNQEELSAGHK
                     TYAVDLPGHGGSTKDVGDMGLRGLAAAVVAFMDTKDIAKAHLVGHSMGGATALLLALE
                     QPDRVASATLVAPAALGDEINMDYINGFIEEKRAKKLRPVLEMLVGDPSLISKDMVED
                     VLKFKRLDGVDAALKKLRDALMPGGKQAGSLRDGLAGLKVPVQILWGEVDQVLPVGHS
                     EGLPASIKVTRFAGVGHMPHMEKAAETNELIRALAG"
     gene            10756..11190
                     /locus_tag="P5558_14970"
     CDS             10756..11190
                     /locus_tag="P5558_14970"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRY25667.1"
                     /translation="MRSPASLFLPGLVLAALAAGAGPATAGDIRSDNRIEGGTWLAED
                     IQSRGAMDYLQTTLQVTSGGGVSGFGGCNRYFGDAEIDGDKIRFGALGATRMACPAAV
                     MDQESRFLAALELVRRWRVEPTGLLHLLDEDGVTVVRFSRQS"
     gene            11277..12059
                     /locus_tag="P5558_14975"
     CDS             11277..12059
                     /locus_tag="P5558_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRY25668.1"
                     /translation="MQAAGWAGKPWAGRAAGLARRLLDLLLPPRCLACGELVEEQGRL
                     CAGCWSSVGFLLGRGCRVCGLPLPPVAAEAAICGHCQAASPVYARARSALRYGGVARQ
                     LILRFKHGDRTDGATTFARWMARAGAELLGDCDLIVPVPLHRWRLLQRGYNQSAVLAQ
                     ALGRRSGKPVLVDALVKLEATPSQQQLGAEARRANIRPELFALGRRGRAAVDGRRVLL
                     VDDVLTTGSTTDACATCLIAGGATAVDVLTLARVAHDPSEPI"
     gene            12065..>12188
                     /locus_tag="P5558_14980"
     CDS             12065..>12188
                     /locus_tag="P5558_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRY25669.1"
                     /translation="MAKVEIYTSPFCPYCIRAKRLLQQKGVAFDEINVLMAAGRR"
BASE COUNT         1912 a         4040 c         4248 g         1988 t
ORIGIN      
        1 gatgagggcg gaagcgagcg ccgtggcgag acggtatcgc aaggggcttc tcagtcgccc
       61 gacgcggcgc gcaggctggc gaggtcggtg atcgtcactg aatgcttgtc cggcaggatg
      121 atcagcccgg ccttgcgcat cagcgtgagg gtgcggctca cggtctcgat ggtgaggccg
      181 aggaaatcgg cgatgtcgcc gcggttcatc ggcagcggca tgggctcgcc ctggcccagg
      241 tccaaccgcc cggcgaggcc gagcaggaag ctcgcgacgc gctcgcgcgc ggtcttgcga
      301 ccgagcagca gcatctgcgc ctgggcggcg gcgagctcgt tgccggcgcg gctgagcagg
      361 cggtgctcga gctcggggtg gtcggccatc agggcgagga ggctgccccg gcgaaagcgg
      421 caggcgacga tgtcggtgac cgcctccgcg ctgtagatat agctgtcgcc ataggcgagg
      481 ccgacgaggt cgcccgggat catgaagccg gtgatctgcc ggcggccgtc cggcagcagc
      541 ttgtagatct tgagcatccc ctcggtgagc gtgaagatgt cctgggcgtg ctcaccctcc
      601 tggatgagct gcgcgccggc gtcgacccgc ctggagccgg cgatggccgc cagcttgccg
      661 agggcgatcg gcgccagggc gccgcagatg gcgatcggcc ggatgccgca attggcgcag
      721 tcgacacgcg cgccgcaaca atgggtttgc tgtcgatcca taattgtgaa gcgatccttc
      781 ccacggccgc ccgtcacgat gccgtatgac cgcggagggg accgagcctg ccgaggctca
      841 tttacgcatt cacgttgaca tgggtcaatg cggcggctcg acaccggggc gacgcttcga
      901 tgatgatcgc gctttcgccc gtcgcatctt cctacatgct gccgattgcc ggcctgccct
      961 ggtattggcg tgatctcgag cacgatcgca tcgacgaggt gctgccgctg gtgctcgccg
     1021 tcgacccgga ccgcaccgag gccccgcact ggcggcggga cgcccaggac cggatcggca
     1081 acgcgtcggc cggcagcggc atcgtcatcg cgcagtgctt cgccggtctg accctggcct
     1141 atttcttcca tgtctgcagc cgggcgccgg acggccggcc gcggctcgtg gtcgaccggc
     1201 tgcgctggat ggagctcggc aggccgcacc gctcgctgga cgcgctcctc gccatcgtgc
     1261 tgcagacggc gacgcggttc gcctgcgccg aggtgctgct ggaggagcgc gcctgtgtcg
     1321 agcggcgggc acgcgcggca ctggagcagc gggcgtccga gacgggcttc acggccacgg
     1381 ccggggcctg gcgacgggtg gtcccgtcgt cgcgctgacc cggccccggg tgccgccctc
     1441 ggttcctgca ggcaccggat ggccggcggg ggcgggggca ttgacaggcg tcggcgaccc
     1501 ctcctaatgg tgcatgaaac aaagttccgg cacggaacgg atgttccgtg cctccaggga
     1561 acaggggagg aggcggcacc ttctcgatcg gcttcggtcg gtcgaatcgg cgcccctgcc
     1621 ggacccctgc ccgcaacagg gaggccgcca tgttctacac cccaaagaat gcgctcttcc
     1681 ggcgggtcgg ccgccgcaag ttcctggagc tcggcggcgg tgccgccacg gcactcggcg
     1741 ccggctcgat cgccgtcggc ctgaactcgg tcgtcacccg ccggccggcc tacgcgcagt
     1801 cgtcggagga ggccaagtgg cgccagttcg agggcaccaa gctcgtcttc atgagcgaga
     1861 acacgccgcc ctccttcgcc attcgtgaga acctccagcc gttctacgac ctgaccggca
     1921 tcgaggtgga gatcctcaca gacgacctgc cggtggtgca gcagaaggtc ggcatcgacc
     1981 tcaggggcgg cagcgccgac ttccatctca actacgtcca ggacaagccg atcggcgcgc
     2041 cgttcgccga tttctacgtc gacctgacca agttcatggc cgacgacacc ctgccgcagg
     2101 acccggacgg gtcaggcgag gatgcctggt tcgagaactt cctgaccgcc tgcggcgtct
     2161 attacgacga ggggcggatc gtcgccttcc cctatgactg cgcggtcgcc tgcaccttct
     2221 accggcagga tctgttcgag cagctcagca aggacttcga ggccgagcac ggctaccgga
     2281 tggagttcaa cgccgacacg acctggcaga acgtccagga tttcgccgcc ttcttcaaga
     2341 agctgcgcga gggtggccgc gaggacatcc cgtacggcta tgcccagcat cagggcagct
     2401 tcgcctggac gacgcagctc gacatccagc ggctcctgtt cgcgaacggc cgctggacgg
     2461 acttcgaggg ggtcgacgac aagctcggca gccgcgagcc cggcccgacg cgctggggcg
     2521 acgagcagtc ggtcctgatc atgcagaagt tcaaggacca ggcggatgtc agccacccgg
     2581 acaacctcgc caacggcacg ctcgagctga acacggtcta ccaggccggg cagatcgcga
     2641 tgcaggtcca gtatcacgag ttcgccgcct cgatcgagga cgagagcacc agccgggcgg
     2701 ccggcggcaa gaccggctac ggcgtctgcc cgctcggtgc caaggagtgg atcgtcaacg
     2761 gcggcgaggc ggtcaacggc accaattgcg gcatcggcgg catcggcatc aatgccgggg
     2821 cgtcggagga gctgcagcgc gcggcctatc tcttcgccat ctggtcgacc tcgaagaaca
     2881 cgcagttcag cgtgctcaag ggcgtcggcg gcacgccgac ccgcaaggcc gtgctggaga
     2941 tcccggaggt ccaggcggcg cggcagcggc cgaccaacat gccgaacgcg ctgaccttcg
     3001 agccggtcta cgactacggc atccgcgacc cgcatttcgt gctcgggccg aagatcccgg
     3061 aggcgaacga gtaccacaag attctggcca ccgaggtgca gcgttgcatt tccggccaga
     3121 cttcggccga ggaggcctgc gaggagattc gctggcagct cgacgacatg cacggtcact
     3181 gagcgatcga ttggcgggcg gccgggccgg cgggtcggtt gatccgccgg cccggtgccg
     3241 aaaaggcggc gggcaggcga gccaggtggg cggacgggca tggcggtgac gacggcaggg
     3301 ggaggacggg gcatcggcgg cggcgcgtcg gccgctgccg gcaagcagca tctgctcgag
     3361 atgcgcgagg gtgcccgggt catcagccac acgccggccc ggctgaacac ggcgccatcg
     3421 cgcgcctact acttctacct gatgctgccg gcgatcatcg tcctcgcctg catcaccctc
     3481 tacccgttct tctggctcgt ctggatgagc ctgcacgacg tcgacgtggg tgccgccgcc
     3541 gacaagtgga acgacttcgc caactggatc cggctcggcc gcgaccgcaa gttccaggac
     3601 ggctggtggc tgctgctgca gtacagcgcc atgtgcatgg tcctgcagat cgggctcggc
     3661 gtgctgctcg cggtgatcct caacggctcg cgccacgaga agctcctggt cacgatcttc
     3721 ctgatgccga tgatgatggc gccggtcgtg gccgggctcg tctggtacta cctctacaac
     3781 tcgaccttcg gctggtacca ctggctgatg cagagtgccg ggatcctcgg cgagcgttcg
     3841 atcctgggcg acccctcgac ggcgatgctc ggcatcgtca tcgtcgacac ctggcagtgg
     3901 acgccgctca tcacgctgat cgtgctggct gggctcaagc gggtgccgcg cgaccagctc
     3961 gaggccaaca tggtcgacgg cgccggcgag atccgcaact tcttccagat caccctgccc
     4021 aatatctatc ccttcctgct gatcgccatc cttctgcggt tcatggacaa tttccgcttc
     4081 atcgaccaca tcctggtgct caccggcggt ggtccggccg acagcaccaa gatcctgccg
     4141 acctacctct tcgacgtcag cttccagttc ttcaagctcg gccggggtgc cgcgatcgcg
     4201 ctcacgctgc tcgccgtcac catcgtgctc ggcctgatcc tggtgcgcgt cttccagagc
     4261 ccgacggcgc agacccggcc ggaggacagc tgatggcctc ctccgacgtc gtccgctacc
     4321 gctcgccgct gcgctcgctg gtcaatgtcc tggtcgcgat cttcctcgtg atctacgtcg
     4381 tctggacggt gctgccgctg ttcgtgatgt tcgtctcgtc cttcaaggac ctgctcgagg
     4441 cattcaagct gccgacggtc ggcgactggt cggggatcac gctgttcttc gacttcaccc
     4501 cgaccgtcaa gcattaccag agcctgttcc tcgaccacaa tttcagcgtc tatctcttca
     4561 actcgatcgt ggcgtcggcc ggctcggcgc tgatctcggt ggtgctgggt gcgatgtgcg
     4621 cctactcgct ctcgcggatc aacttccgcg gcaagcagga tctcttcttc tggatcatca
     4681 gcacccggat ggcacccgtg gtcgccgtga tggtgccgct ctacgcgatc ttcaggggtg
     4741 cggggctcgt cggcacgatc cccggcctga tcctggccta caccaccttc aacctgccgt
     4801 tcgccatctg gatgctgaag ggcttcttcg acaacgtgcc ctacgccatc gaggaggcgc
     4861 agatgtgcga cggggcgacg cgctttcgcg cgttcctgtc gatcctgccg ctggtggcgc
     4921 ccggcgtcgg ggcgttcctc gtcctctgca tcctcttcgc ctggaacgac ttcctcttcg
     4981 cctcgatcat cggctcgggc ggggccaaga ccctgcccgt cgccaccaag gagctggtgc
     5041 agccgcagaa catccagtgg ggcagcatca tggcggccgg ggtcgtcacg accctgccga
     5101 tgatgttcct cggccttctg atccgccgct atctcgtggc cgggctcacc atgggcgctg
     5161 tgaaagagta gagcttcgat ggcctctgta acgatcgacc acgtctggaa gtattacggc
     5221 aagaccgagg ccgtgaagga tctcaacctg aaatgcgccg atggcgagtt cctctcggtc
     5281 ctcgggccgt cgggctgcgg caagtcgtcg acgctccgga tgctcgccgg gctcgagcac
     5341 atctcggccg gcgacatccg cttcggcgag cggcggatca acgacctgcc gcccaaggat
     5401 cgcgacatcg cgatggtctt cgagaactat gccctctacc cgcacaagac ggtgcgggag
     5461 aacatcgcca actcgctgat gctgcgcggc gccagccggg cgacgatcga gcagaaggtc
     5521 aagaaggcga cggagctcct gcagatcggc catctgctcc agcgcaagcc gcaggagctc
     5581 tcgggcgggc agaagcagcg cgtcgcgatc ggccgggcga tcgtccgcga gccgcagctc
     5641 ttcctcttcg acgagccgat cgcccatctc gacgcgaagc tgcgggcgca catgcgcggc
     5701 gagctcaaga tcatgcagcg cgagctcggc acgacgatga tctacgtcac ccacgaccag
     5761 ctcgaggccc tctccatggc cgaccggatc gcggtgatgc acgagggcgt cctgcagcag
     5821 ttcggcacgc cacaggagat ctacgatcat ccggtgaacg aatgggtcgc gggcttcgtc
     5881 ggcgagccgc cgatgaactt cgtcgactgc ctgatcgagg accggggcgg cgtgctgcat
     5941 gccgtgcacc cgagcttcgc cgtgccggta acggggtcgc aggaggcgat cctgcgcgat
     6001 tcggcgcacc gggccgaggt gcggatgggc attcgcccgg accagatcgg gatcgtcacc
     6061 ggcgacggtg gcgacgcggt ggcggcgcag attctgctca ccgagcccct gggcggtgat
     6121 atgctggtcg acgctgccgt tggccaggag aagttcctgg tcaagaccaa gccggatttc
     6181 caggggcggc gtggcgatgc ctgccggctg gccttcgaca ccgggcgctg gcatctcttc
     6241 gaccgggata ccgggcttgc ctatttctga gcgacgccat gtcgggtgcg acgaccgtgg
     6301 gtagagtctt ttgccgggag gacgtcctat gcaggccctg aggtttcacg ccgccaagga
     6361 tcttcgcctc gacgacatcg cggcgccgcc ggcgcgtccg gccgacgacg aggtggtgat
     6421 ccgcaaccgt ttcgtcggca tctgcggcac cgatctccac gagtacagct acggcccgat
     6481 cttcatcccg accgagccgc accctttcag ccacgccaag gcgccgcagg tgctggggca
     6541 cgagttcggc ggggtggtcg aggcggtggg cgccggcgtc acgtcggtcg cgcccggcga
     6601 ccgggtttcg gtgcagccgc tcctgatgcc gcggaccggc gagcactatg ccgatcgcgg
     6661 gctctttcac ctctcgggca gcctcgccct cgtcggcctg agctggggct ggggcggcat
     6721 ggccgagttg gcggccgtca aggagtacaa cgtcttcaag gtgcccgatg cgctgaccga
     6781 cgaggaggcg gcgctggtcg agcccacggc ggttgccgtc tatgcctgcg accgaggccg
     6841 tgtggcagcc ggcaacagcg tgctggtgac cggtgcgggg ccgatcggtg ctctcgtgct
     6901 gctcgccgcc cgggcggcgg gagccgcgcc gctcttcgtc tcggacgtca atgacgcgcg
     6961 gctcgacttc gtccgcgcga tgctgccgga cgtggtcacc atcaacccgt cgcgggagaa
     7021 gccgggcgat gtcgtgcgcg ccgccaccga cggccgggcc ggcgtcgatg tcgcgatcga
     7081 atgcgtcggc aatgagcacg cgctcaagga ttgcctcgat gcggtgcgca agcagggtgt
     7141 catcgtccag accggcctgc acccgcacga gaacccgctc gactggttcc aggtcacctt
     7201 caaggacatc gatatccgcg gctcctggtg ctacccgacc tatctctggc cccgggtgat
     7261 gcggctgatc gcgtccggcc tcctgccggc gaagaagatc gtgaccaaaa ggatcggcct
     7321 cggcgaagcc gtgccggccg gcttcgaggc gctgctcgat ccgggcggga cccaggtgaa
     7381 gatcctgatc gacttgtcga aatgacgggg ctggcctaga ggtgcgcggt agcgctggga
     7441 gcttggccga tcgccggccg gacttgcgtc agggacgaag aacgagggga ggcgaccgtg
     7501 tcgcacgcgg cctgcagggt tcgtcgcgca cgcctccctc gcgaatttca gggcatgcca
     7561 accgggagga cagaccatgc agttaagtcg tgacgatctg ctcgaagcct atcgcaagat
     7621 gcggatgatc cgcgagttcg aggacgcggt gcacgaggag ttctcggccg gcaacattcc
     7681 gggcttcgtg catctttatg ccggggagga ggcctcgggc gtgggggtgt gcatgcatct
     7741 cgacgaccgc gacgccatcg cctcgacgca tcgcggccac ggccactgca tcgccaagaa
     7801 ctgcgacgag cgtgccatga tgttcgagat cttcggccgc aaggaaggcc tgtgcggcgg
     7861 caagggcggc tcgatgcaca tcgccgacat gtcgaagggc atgatggggg ccaacggcat
     7921 cgtcggcggt ggcccgccgc tgatctgcgg tgcggccctc tcggccaagt atctcaagac
     7981 cggcggcgtc gcgatcgcgt tcgtcggcga cggcgcctcg aaccagggca ccacgttcga
     8041 gagctacaat ctcgccaagg tctggaacct gccggcgatc ttcgtcgtcg aggacaacgg
     8101 ctacgccgaa tcgacggcct cgaagtggtc cgtggcgggc agccaggtcg atcgggccaa
     8161 gggcttcggc atgccgtcct atgacgtcga cgggcacgat ttcttcgccg tctgggaagc
     8221 tgcgcgcgag gccatcgagc gggcccgcaa tgacggcggc ccgtcgctca tccactcgcg
     8281 gctgaaccgc tactacggcc acttcgaggg tgacgcgatc agctacaagg cgccgggcga
     8341 gatcgagaag gtcaaggccg agaaggacgc gatcatgctc ttccgcaacc gggtctcgga
     8401 ggccggcctg ctcgacggcc cggccatgga cgcggtggac aaggcctgcc gcgagcggat
     8461 cgcccaggtt ctcgccgagg ccaaggccgg cgccatgccg accgaggccg acctgctcac
     8521 cgacgtctac acctcggcct attgaggagc gccagtcatg ccgaagaaga cctatcgtca
     8581 agcgatcaac gaggcgctcc gtcaggagat ggagcgcgac caccgcgtca tcctgatggg
     8641 cgaggacatc tcgggcggca tgggtgcccc gggtgagcag gatgcctggg gtggcccgtt
     8701 gggtgtcacc aaggggatca tgccggtctt cacccgcgac cgggtgctgg acacgccgat
     8761 caccgagagc gccttcatcg gtgccgcggc cggtgccgcg gccaccgggc tccggccggt
     8821 ggcggagctg atgttcgtgg acttcatggg cgtctgcttc gaccagatct tcaaccaggc
     8881 ggccaagttc aagtacatgt tcggcggcaa ggcggtcaca cccctgacca tccgcaccat
     8941 gtacggcgcc gggttccggg cggcgtcgca gcacagccag tgcctctacc cgctcttcac
     9001 ccacatcccg ggtctgaagg tggcgctgcc ctccaacgcc tacgaggcga agggcctgct
     9061 ggcgacggcg atccgcgacg acgacccggt gatgttcttc gagcacaagg ccatgtacga
     9121 cgacgaggcc gaggtgccgg acgaggccta caccatcccg ttcggcgagg ccaacgtcac
     9181 ccgcgagggt gacgacgtga ccatcatcgc cttcggccgg atggtgaact tcgccaacga
     9241 ggcggccgac aatctcgaga agtcggggat ctcgtgcacg gtggtcgacc cgcgcacgac
     9301 gtcgccgctc gaccgggaca ccatcctcga gacctgcgag gagaccggcc gggtcgtggt
     9361 ggtcgacgag gcgcatccgc gctgcaacat ggcgaccgac atcgccgcca tgtgcgcgca
     9421 ggagatcttc ggtgccctga aggcgcccgt gaagatggtg accggctatc attgcccggt
     9481 ccccttctcg ccggcgctcg aggatctcta tgtgccgaac gccgccaaga tcgaggcggc
     9541 ggtgcgcgag gtggccggca agggccactg agcctttccg ccgaggcacg cccgggcgcg
     9601 caacccgtgc ccgggcgttg ctgttttcag atgcagagca agaacgagga gggggtgccg
     9661 tgtccgacgc cattcagatg atcgtgatgc cgaaatgggg cctcgccatg caggaaggca
     9721 tggtggccca gtggcatgtc gccgagggtg cgaagatcac caaggggcag gagatcgtcg
     9781 acatcgagac gtcgaagatc gccaacgtgt tcgagagccc catcagcggc acgctgcgcc
     9841 ggctggtggt caaggacggc gagaccgtgc cggtcgccta cctgatcggc gtggtcgccg
     9901 aggaatcggt gacggatgcc gagatcgatg ccttcgtcga agacttcaag gccaatttcg
     9961 tcttcgatgc cgccggcgcc ggcggcccgg agccggagga cgcgaccacc gcggccggcc
    10021 gcatccgcta tctcgagatg ggcgacggcg agggtgcgcc catcgtcttc atccacggct
    10081 atggcggcga cctcaacaac tggatgttca accaggagga gctgtcggcc gggcacaaga
    10141 cctatgccgt cgacctgccc gggcatggcg ggtcgaccaa ggacgtgggc gacatgggcc
    10201 tgcgcggcct cgccgccgcc gtggtcgcgt tcatggacac caaggacatc gcgaaggcgc
    10261 acctggtcgg ccattcgatg ggcggggcca cggcgctcct cctggcgctc gagcagccgg
    10321 atcgggtggc gagcgccacg ctggtggcac ccgcagcgct cggcgacgag atcaacatgg
    10381 actacatcaa cggcttcatc gaggagaagc gggcgaagaa gctccggccc gtcctcgaga
    10441 tgctggtcgg cgacccgtcg ctgatctcca aggacatggt cgaggacgtg ctgaagttca
    10501 aacggctgga cggggtcgac gcggcgctga agaagctgcg cgatgcgctg atgccgggcg
    10561 gcaagcaggc cggcagcctg cgcgacgggc tggccgggct gaaggtgccg gtgcagatcc
    10621 tgtggggcga ggtcgaccag gtcctgccgg tcgggcacag cgagggtctg ccggcgtcga
    10681 tcaaggtgac ccgcttcgcc ggcgtcggcc acatgccgca catggagaag gcggcggaga
    10741 ccaacgagct catccgtgcg ctcgccggct agcctcttcc ttcccggtct ggtgctggcg
    10801 gccctggccg ccggcgccgg gccggccacg gcgggggaca tccggagcga caaccggatc
    10861 gagggcggga cctggctcgc cgaggacatc cagtcgcgcg gcgcgatgga ctacctgcag
    10921 accacgctgc aggtgacgag cggtggcggg gtcagcggct tcggcggctg caatcgctat
    10981 ttcggcgatg ccgagatcga tggcgacaag atccgcttcg gtgcgctggg cgcgacgcga
    11041 atggcgtgcc ccgcggcggt catggaccag gagagccggt tcctcgccgc cctcgagctg
    11101 gtccgccgct ggcgcgtcga gccgaccggc ctcctgcacc tgctcgacga ggatggggtg
    11161 acggttgtca ggttcagccg gcagtcgtag gccgcccgcg gtgggaggtg ggcgagcttg
    11221 cctatcgggc gattgtgctg catatcattc aaccattgcg tgtgctggat ggcggaatgc
    11281 aggctgctgg atgggctggg aagccttggg ccggccgggc ggcgggtctg gcacggcggc
    11341 tgctcgatct gctgctgccg ccgcgctgcc tcgcctgcgg cgagctggtc gaggagcagg
    11401 ggcggctctg tgccggctgc tggtcctcgg tcggcttcct gctcggccgc ggctgccggg
    11461 tctgcggcct gcccctgccg ccggtggcgg ccgaggcggc gatctgcggg cattgccagg
    11521 cggcctcgcc cgtctacgcg cgggcgcggt cggcgctgcg ctatggcggg gtggcgcgcc
    11581 agctcatcct gcgcttcaag catggcgacc ggaccgacgg ggccacgacc ttcgcccgct
    11641 ggatggcgcg cgccggcgcc gagctgctcg gggattgtga cctcatcgtg ccggtgccgc
    11701 tgcatcgctg gcggctcctg cagcgcggct acaaccagtc ggcggtgctg gcgcaggcgc
    11761 tggggcggcg cagcggcaag ccggtgctgg tcgacgccct ggtcaagctc gaggctacgc
    11821 cgtcccagca gcagctcggg gccgaggcgc ggcgggccaa tatccgcccc gagctcttcg
    11881 cgctcggccg gcgcggccgg gcggcggtcg atggccggcg ggtgctcctg gtcgacgacg
    11941 tgctgacgac cggcagcacc accgatgcct gtgcaacctg cctgatcgcc ggcggggcca
    12001 cggcggtcga cgtgctgacg ctggccaggg tagcgcacga cccgagcgag cctatatgac
    12061 gctgatggcc aaagtcgaaa tctacacctc gcccttttgc ccgtactgca tccgcgccaa
    12121 gcgcctcctg cagcagaagg gcgtcgcctt cgacgagatc aacgtcctga tggcggccgg
    12181 gcggcggc
//
LOCUS       DAOTFI010000146        12064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6292_length_12064_cov_7.832709, whole genome shotgun sequence.
ACCESSION   DAOTFI010000146 DAOTFI010000000
VERSION     DAOTFI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6292_length_12064_cov_7.832709"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /gene="rho"
                     /locus_tag="P5558_14985"
     CDS             complement(<1..887)
                     /gene="rho"
                     /locus_tag="P5558_14985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRY25670.1"
                     /translation="MSLTELKRRPAAELLRYAEEIGIENASTLKKGDLVFAVLKELAM
                     TGVPITGDGVLEILQDGFGFLRSPDMNYFAGPDDVYVSPSQIRRFALRTGDIVEGQVR
                     APKEGERYFALLRANTINLDPPEVARHRIHFENLVPYFPTRKLNLEVLGKKDVSTRII
                     DIVCPLGKGQRALIVAPPRTGKTVLMQNIATAISANHPEVYLIVLLIDERPEEVTDMQ
                     RTVKGEVVSSTFDEPATRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVV
                     PSSGKVLTGGVDANALQRP"
     gene            complement(1561..1992)
                     /locus_tag="P5558_14990"
     CDS             complement(1561..1992)
                     /locus_tag="P5558_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRY25671.1"
                     /translation="MADLALWLLALHIMAFAAWMAAMWYLPRLLIYHCDAVVGGEASA
                     TFKVMERRLLKAIGTPAMVATFIFGLWLATLQSQWDQGWLHAKLALVLGISGCHGLIA
                     RDVRRFAADERPRSARWYRVFNEVPTLLFVGIVVLVVFRPF"
     gene            complement(2141..3184)
                     /gene="hemE"
                     /locus_tag="P5558_14995"
     CDS             complement(2141..3184)
                     /gene="hemE"
                     /locus_tag="P5558_14995"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRY25672.1"
                     /translation="MSTDSPKRLLRVLRGEPVDRAPVWLMRQAGRYLPEYRALRRKTG
                     GFLELCYDPVLAEEVTLQPIRRFDLDAAILFSDILVVPDALGADVGFHEGEGPRLTPL
                     AHRSDLGRLSTERLHEHLEPVYETLRRLRRSLPGEVTLIGFAGAPWTLAAYMVEGGGS
                     KDWAVARRLARVEPRLFGDLVDLLTKAVIAFLERQVAAGAEVLQVFDSWAGALPPDER
                     ERWVTAPLARIVAELGERCPGVPVIVFARGVGPALVDLAAAIRPAGLGIDTTIDPAWA
                     AASLPGEFCLQGNLDPIALVAGGDTMRRAADRILAAWRERSFIFNLGHGVVPETPPEN
                     VLDLVRHLKSGSD"
     gene            3627..4544
                     /locus_tag="P5558_15000"
     CDS             3627..4544
                     /locus_tag="P5558_15000"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRY25673.1"
                     /translation="MRLHLHLISDSTGETVTTVARAAVSQFEDVVPVEHVWFFVRTRA
                     QLEKVLTIVEALPGMVIYTLVSPELRFLLHERCRAFAVPCVPVLDPVMNALTGLLGSA
                     GRPQIGRQHAMDDDYFSRIAAMDFTMRHDDGQFPEELDEADVVLVGVSRTSKTPTCVY
                     LAHRGIKAANVPLVLDLPQLAILEQLKRPLIVGLTINPEQLMHIRRNRQQMLGLEGDE
                     TSRFGGRYADLDHVREELRYARRLFARHGWPEIDVTKRSVEETAATIYQLVQARAFPE
                     IGALVETGRAAESGAESGQVESAARSSDA"
     gene            4589..5326
                     /locus_tag="P5558_15005"
     CDS             4589..5326
                     /locus_tag="P5558_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25674.1"
                     /translation="MKQLSTLGRRRRLARRQFIWNMARLLIVAALLVLFAVAGYQAGI
                     SQSSHEGERLAADLAAAAARNLELTERLAMAEDRAGRLETQLQALRLDYAANVPPAEF
                     KPLLALLQARVRAGVDRARLAFVIEQAGREQTCERGVQTARLLVRTPVSTALENSVSF
                     ADGRITVSGDGRPARDEAGSPLDWYDPGQPVTLRFLTIAGDLSRVVGRLPLVHSVILG
                     DREWRFQATASREPGRIEVVAQSCDYP"
     gene            complement(5317..6390)
                     /gene="dinB"
                     /locus_tag="P5558_15010"
     CDS             complement(5317..6390)
                     /gene="dinB"
                     /locus_tag="P5558_15010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRY25675.1"
                     /translation="MRKIVHIDMDAFYAAVEQRDQPALRGLPIAVGGGQTRGVVMTAS
                     YEARRFGVRSAMPGARAARLCPELVFVRPRFEVYKAVSRQIRAIFRQHATLVEPLSLD
                     EAYLDVTEPPNGPLPAVAIARAIKDQIRDETGLTASAGVSCNKFLAKLASDMEKPDGL
                     CLIRPAQVLDVLAELPIERFFGVGPATASRLRAAGIRRGADLQALDEAAAVHRLGRSG
                     AHYWRLAQGRDDRPVVPDRSRKSLSVETTFDQDIAGVTELTAALEELARDLAARLAQS
                     GYQALTVHLKIKYLDFRISTRQTTLREAPRSAESLAAVGRHLLLRAPLAAPVRLLGIG
                     VGGATTEAGQLTLGLDPQPADQG"
     gene            6630..7994
                     /locus_tag="P5558_15015"
     CDS             6630..7994
                     /locus_tag="P5558_15015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRY25676.1"
                     /translation="MNQASPGCTLRGVVLVAVLLAAGGLGADTARGEDLREALTAAYL
                     NNPRLASGRDQLRATDELVPQALAAGRPSLFLDGRVEGVTSDTDVAERDNRLGRGVAL
                     SLRQPLYAGGGIEAGIRRAENQVRAERAFLLSTQQSVLLASVDAYTATWRDRAVLELA
                     RTNEMRLRRQLEATRDRFEVGEVARTDVAQAEARLSRARSDVELATADLAASTAAYRD
                     VIGIDPGVLVDPQPLGRLPSSEPEAQALAAANPDITTASFNLRAARDNVDVAFADILP
                     SLTLTGDVSYDQQPSQALDSSNDARIGLNLSVPLFQGGADYSRVRQNRQLVSARRNDL
                     ENTLRGVQRSVTTAWERVIAARAAIDALESEVRANGIALEGVQEEALVGQRTVLDVLD
                     AENELFVSQVDLVRARREWVLATYQLKSAIGELTVARLDLPVEPFDDERYYNRVRNRL
                     FGLE"
     gene            8158..8649
                     /locus_tag="P5558_15020"
     CDS             8158..8649
                     /locus_tag="P5558_15020"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HRY25677.1"
                     /translation="MTSSGKRCLVLASALATSFVVPALADTDTDVLTVMVTVQEACAI
                     SGSTLDFGTYTGGQQSALNGQGQISYTGCAAGTLVIDFDGGTSGDVQARRMASNAGGT
                     LGYQLYRNSSRTQVWGTGANAQQVQLLQAGAGNIPVYGSIPGGQAAAAGSYTDTVNVT
                     LTF"
     gene            complement(8732..10192)
                     /gene="hydA"
                     /locus_tag="P5558_15025"
     CDS             complement(8732..10192)
                     /gene="hydA"
                     /locus_tag="P5558_15025"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRY25678.1"
                     /translation="MSLVIQGGTVVNADQSERADVLIEGGTIRAVGPDLDVPTGAEVI
                     DAGGALVIPGGIDPHTHMQMPFMGTVAIEDFYSGTCSGLAGGTTMIIDMCIPSPQQSL
                     TAALDDWSDWASKAVADYGFHVAITWWSERVEQQMAEVIDRGVNSFKHFMAYKGALMV
                     DDEILFQSFSRCRELGALPLVHAENGEAVFRLQQSLLAQGITGPEGHALSRPPHVEAE
                     AAIRAIMLAKITGVPLYIVHTSCRETVEAIARARSEGQRVFGEPLAQYLCLDDSVYQS
                     PDWAYAASRVMSPPFRSKEHQDALWAGLMNGQLQVTATDHCTFLAEQKAKGRDNFTMI
                     PNGTGGLEERLKILWHFGVNAGRLTPEEFVAVTSTNCAKIFNVYPRKGAVRAGSDADL
                     VVWDPKARHTIRQASHQSKLDVNVFEGIEVEGFPAITIANGKVVWRNGQLDVTRGAGR
                     HVERPPMSPVMAAQARRNDLTRPHAVARQPSAQQTP"
     gene            10311..10682
                     /locus_tag="P5558_15030"
     CDS             10311..10682
                     /locus_tag="P5558_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY25679.1"
                     /translation="MKAGNRTLGLRLRVVLEPDIAIGPGKADLLEGIGETGSIAAAGR
                     RMGMSYKRAWLLVETMNRCFREPLVRTSRGGSARGGAVLTPAGAEVLALYRRMEERSA
                     RAVEAELAGLQALCRDRTPEE"
     gene            10765..11538
                     /gene="modA"
                     /locus_tag="P5558_15035"
     CDS             10765..11538
                     /gene="modA"
                     /locus_tag="P5558_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY25680.1"
                     /translation="MRAVQRAVVIVAVAALGLAGTGQARAAEVTVFAAASLKNALDEV
                     AAGWQAATGNTVSISYAGSARLARQIEQGAPADLFISANTAWMDHLDEAGLIDEASRR
                     DLLGNRLVLVAHGRDVAPVDLAPGADLAHRLGDGRLAMALVDAVPAGIYGKAALEELG
                     LWASVADRTAQTDNVRAALALVALGEAPLGIVYATDAVAEDGVTVVATFPEDSHPPIV
                     YPVALTVEGTRSAAVDLLAWLGSAAARGSFERQGFSVLD"
     gene            11547..>12064
                     /gene="modB"
                     /locus_tag="P5558_15040"
     CDS             11547..>12064
                     /gene="modB"
                     /locus_tag="P5558_15040"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRY25681.1"
                     /translation="MLQLSPEEWTAVALSIRVAGWATLLSLPLGIATALLLARRRFWG
                     RELLNGLVHLPLILPPVVTGYMLLLLFGRRGPIGSLLEETFGIVLAFRWTGAVLACAI
                     MAFPLMVRAIRLAVEAVDPKLEQAAGTLGANRLWTFATITLPLILPGVIAGAILAFAK
                     AMGEFGATITFVS"
BASE COUNT         1767 a         4199 c         4210 g         1888 t
ORIGIN      
        1 ttcggccgct gcaacgcatt ggcgtcgaca ccgccggtca ggaccttgcc cgaggagggg
       61 acgaccgtgt tgtaggcgcg gccgaggcgg gtgatggaat cgagcaggat gacgacgtcg
      121 cgtccgtgct cgaccagccg cttggccttc tcgatcacca tctcggcgac ctggacgtgg
      181 cgcgtcgccg gctcgtcgaa ggtcgagctg acgacctcgc ccttcaccgt ccgctgcatg
      241 tccgtcacct cctccggccg ctcgtcgatg agcaggacga tgaggtagac ctccggatgg
      301 ttggccgaga tcgcggtggc gatgttctgc atcaagaccg tcttgccggt ccgtggcggg
      361 gccacgatca gggcgcgctg ccctttgccc agcgggcaga cgatgtcgat aatgcgcgtg
      421 ctgacgtcct tcttgccgag cacctcgagg ttcagcttgc gggtcggaaa gtaggggacg
      481 aggttctcga agtggatgcg gtgacgcgcc acctccggcg ggtcgagatt gatcgtgttg
      541 gcacgcagca aggcgaagta gcgctcgcct tccttcggtg ccctcacctg accctcgacg
      601 atgtcgccgg tgcgcagcgc gaagcggcgg atctgcgagg gcgacacata gacgtcgtcg
      661 ggaccggcga aatagttcat gtcgggcgag cgcaggaagc cgaagccgtc ctgcaggatc
      721 tccagcacgc cgtcgccggt gatcggcacg ccggtcatcg ccagctcttt caggacggcg
      781 aagacgaggt cgcccttctt cagcgtgctg gcgttctcga tgccgatctc ctcggcgtag
      841 cgcagcagct cggccgccgg ccggcgcttg agctccgtca ggctgatcgg cggcagatcg
      901 gagcgcacct cgggatagtc gcgctcgcgc tcccggccga actcgcggtc ccggtcgcga
      961 ttgccgtcac gctcacgatt gtggtcgcgg tcccggttgc tgtcacggtc ccggggccgc
     1021 tccgcccgct cgcggtcgcc ccggtcgcga tcccggggcc ggtcccgctc gcgcggctcg
     1081 cgggcctccc ggggctccct cggctcgcgc atctcgcggg ccgggcgcgg ggcggattcc
     1141 tccggctcga ccatgccgaa gagctcggca ggcaggccga tcaccccgtc gtcggtgtcg
     1201 tcgccctcag cattgtcgcc ggcgcccatg ccctcctcga tcgcggcgag cagcggctgg
     1261 tcttcctcga cacggcgagc cggccgcgcc cgagtgcgct tgcggcgggc cggtcggggc
     1321 gcgctctcgc cctcggcggg cggtgacgcc tcaccgtcgc cgtcgctatc ggaccctttt
     1381 gcgccggagc ccgttgcacc ggacggggtg tccgccgcgg tctgcccggg cgtggtcggc
     1441 tcgggcgact tgtcgttcgc gtcgtcacgc ggcgtttcgg ccatcatgtc ctcggtgtcg
     1501 tgaatcaatt ctatctcgat tgttgcgttt gctggaattt ctggcgctgc ggcccacggc
     1561 tcagaacggc cggaagacca ccagaacgac gatgcccacg aacagcaggg tcggcacttc
     1621 gttgaagacg cgataccagc gggccgatcg cggccgctca tcggcggcga accgcctgac
     1681 atcccgtgcg atgagcccgt ggcagccgga tatcccgaga accagcgcca gcttggcgtg
     1741 cagccacccc tggtcccact ggctctgcaa ggtggccagc caaaggccga aaatgaaggt
     1801 ggcaaccatc gccggcgtgc cgatcgcctt cagcaggcgc cgctccatca ccttgaaggt
     1861 cgccgatgcc tcaccgccta ccaccgcatc gcaatgatag atcaacaggc gcggcagata
     1921 ccacatcgcc gccatccagg cggcgaaggc catgatatgg agcgccaaca accagagagc
     1981 gaggtcagcc aggactgttc accatgcgct gcaccacagg tgcgggtcgc cagagagagg
     2041 ccgatttcgc gacaagggaa atcgtcagtg cgccaattgc tggggaccaa ctcattgttc
     2101 gggggtggcg tggccgaacg ccacgcaatc gagtataatg tcagtcgctg ccggatttca
     2161 agtggcgaac gagatcgagc acattctcgg gtggcgtttc cggcacgacc ccatgcccga
     2221 gattgaagat gaacgaacgc tcgcgccagg cggccaaaat gcgatcggcg gcgcggcgca
     2281 tcgtgtcgcc gccggcgacg agagcgatgg ggtcgagatt gccctgcagg cagaactccc
     2341 cgggcaggga ggcggccgcc caggccggat cgatcgtcgt gtcgatgccg agaccggccg
     2401 ggcgaatggc tgcggcgagg tcgaccagcg ccgggccgac accacgcgcg aagacgatca
     2461 ccggcacgcc agggcagcgc tcgcccagct ccgccacgat ccgtgccagg ggtgcggtca
     2521 cccagcgctc acgctcgtcc ggcggcagcg ccccggccca gctgtcgaag acctgcagga
     2581 cctcggctcc ggcggccacc tggcgctcga gaaaggcgat gacggccttg gtcaggagat
     2641 cgacgaggtc gccgaagagc cgtggctcga cccgggcgag ccggcgggcc acggcccagt
     2701 ccttggagcc tcccccttcg accatgtagg cggcaagggt ccagggggca ccggcgaagc
     2761 cgatcagcgt cacctcgccg ggcagcgagc ggcgcagccg gcgcagcgtc tcgtaaaccg
     2821 gctcgaggtg ctcgtggagg cgctcggtcg aaagccggcc gagatcactg cgatgagcca
     2881 ggggcgtcag ccgcggtccc tcgccctcgt gaaacccgac atcggcgccg agcgcgtcgg
     2941 gcacgacgag gatatccgag aacaggatgg cggcatcgag gtcgaaccgc ctgatcggct
     3001 ggagcgtcac ctcctcggcg agcaccggat cgtagcacag ctcgagaaag ccgcccgtct
     3061 tgcggcggag tgcccgatac tctggcagat agcggccggc ctggcgcatc agccagaccg
     3121 gtgcccgatc gaccggctcg ccgcgcagga cgcgaagcag acgcttcggg ctatcggtgc
     3181 tcaacgctcg atccacgctg gggttcggtc actcttgctt aaccttaaga gagaaataag
     3241 gagatggagg ttgtagtatg gccgtggaca acgaggatta gtcgccatcc acgaacgatc
     3301 aacacgattt gggcgtttgg gccgggcggc cgggcggctg tggacaacgg gccacgccgg
     3361 cgtcgtccct ggcgctggca cgaggctttt cgaccgtggt cctgcctttg gcaaggcttg
     3421 gagaagtgct ggaaaagcac cgggcggagc ggcttgtccg ccggcccgcg aatggccctc
     3481 gatggcgcga gtcgggactg gacggcgagc ggccgatcgg ggagcattcg ggagtcgctg
     3541 gcggtgcctc gggataaccc ggggcgctgt ccacagcttc ctccacgcct cctccgttgc
     3601 cctgtccctg cgaggaacct gagcccgtgc gcctgcatct ccatctgatc tccgattcca
     3661 ccggcgagac cgtgaccacg gtggcgcgcg ccgcggtttc gcagttcgag gacgtcgtgc
     3721 cggtcgagca tgtctggttc ttcgtgcgga cccgggccca gctcgagaag gtgctgacca
     3781 tcgtcgaggc cctgccgggc atggtcatct acaccctggt ctcccccgag ctgcgcttcc
     3841 tgctgcacga gcgctgccgg gccttcgccg tgccctgcgt gccggtcctc gatcccgtga
     3901 tgaacgcgct taccggcctg ctgggctcgg ccggacggcc gcagatcggt cgtcagcatg
     3961 ccatggacga cgactatttc tcgcgcatcg ccgccatgga tttcaccatg cggcacgacg
     4021 atggccagtt tccggaggag ctcgacgagg ccgacgtcgt gctggtcggc gtgtcccgga
     4081 cctcgaagac gccgacctgc gtctacctcg cgcatcgcgg catcaaggcg gccaacgtgc
     4141 cgctggtgct cgatctgccg cagctcgcga tcctcgagca gttgaagcgg ccgctcatcg
     4201 tcgggctgac gatcaacccg gagcagctca tgcatatccg tcgcaaccgc cagcagatgc
     4261 tcggcctgga gggggacgag acgagtcgct tcggtggccg ctacgccgat ctcgaccatg
     4321 tgcgcgagga gctgcgctat gcgcgccggc tgttcgcgcg gcacggctgg ccggaaatcg
     4381 acgtgaccaa gcgatcggtt gaagaaacgg ccgcgacaat ctatcagttg gttcaagcac
     4441 gggccttccc cgagatcggc gcgctggtcg agacgggtcg ggcagcggag tccggggcgg
     4501 agtcggggca ggtcgagagt gccgcccgtt cgtcggacgc gtgacgcgag caacctccgg
     4561 gcggtttcca ggtgagctga tcggctgaat gaagcagctt tcgaccctcg gtcgtcgtcg
     4621 ccgcctggca cggcggcagt tcatctggaa catggcgcgg ctgctgatcg tcgccgcgct
     4681 tctggtgctg ttcgccgtcg ccggctacca ggcagggatc tcccagagca gccacgaggg
     4741 cgagcggctg gctgccgatc tcgccgccgc ggcggcgcgc aatctcgagc tcaccgagcg
     4801 gctggccatg gccgaggatc gcgccgggcg gctggagacg cagctccagg cgctccgcct
     4861 cgactacgcg gcgaacgtcc cgccggcgga gttcaagccg ctcctggcgc tgctgcaggc
     4921 ccgggtccgg gcgggagtcg accgcgcgcg cctggccttc gtgatcgagc aggccggccg
     4981 cgagcagacc tgcgagcgcg gggtccagac cgcgaggctc ctggtgcgca cgcccgtgag
     5041 cacggcgctc gagaattcgg tgtcgttcgc cgacggtcgg atcaccgtga gcggcgacgg
     5101 gcggccggcg cgggacgagg ccgggtcgcc gctcgactgg tacgacccgg gacagccggt
     5161 caccctgcgc tttctgacca tcgccggcga tctcagccgc gtcgtcgggc ggctgccgct
     5221 ggtccactcg gtcatcctgg gcgatcgcga gtggcgattc caggcgacgg cctcgcgcga
     5281 gccagggcgg atcgaggtgg tggcgcagag ctgcgactat ccttgatcgg caggttgcgg
     5341 gtcgagcccg agggtgagct ggcccgcctc ggtcgtggcg ccgccgacac cgatcccgag
     5401 cagccggacc ggtgcggcga gcggcgcccg cagcaggaga tgccggccga cggcggcgag
     5461 cgactcggcc gagcgtggtg cctcgcgcag cgtggtctgc cgggtgctga tcctgaaatc
     5521 gaggtacttg atcttgaggt gcaccgtcag cgcctggtag ccgctttgcg ccagccgggc
     5581 ggcgagatcc cgggccagct cctcgagcgc cgctgtgagc tcggtgacac cggcgatgtc
     5641 ctgatcgaac gtggtctcca cgctcaggga cttgcggctc cggtccggca cgaccgggcg
     5701 gtcgtcccgg ccctgggcga gccgccagta gtgcgcgccg ctgcggccga ggcggtgcac
     5761 ggcggccgcc tcgtcgagcg cctgcaggtc cgcgccccgc ctgatgccgg cagcgcgcag
     5821 ccggctggcg gtcgccgggc cgacaccgaa gaaccgctcg atcggcagct cggccagcac
     5881 atccagcacc tgcgccgggc ggatcaggca gagcccgtcg ggcttctcca tgtcggacgc
     5941 cagcttggcg aggaacttgt tgcaggagac gccggccgag gcggtgaggc ccgtctcgtc
     6001 gcggatctgg tccttgatcg cccgcgcgat ggcgaccgcc gggagcggcc cgttcggtgg
     6061 ctcggtgaca tcgagatagg cctcgtcgag ggagagcggc tcgaccagcg tggcatgctg
     6121 ccggaagatc gcccggatct ggcggctcac cgccttgtag acctcgaagc gcgggcgcac
     6181 gaacacgagc tcgggacaga gccgggccgc ccgcgcgccc ggcatcgccg agcgcacgcc
     6241 gaaccgcctc gcctcgtagc tcgcggtcat cacgacgccg cgcgtctgac cgccgccgac
     6301 ggcgatcggc aggccgcgca acgccggctg gtcgcgctgc tcgaccgccg cataaaaggc
     6361 gtccatgtcg atgtggacga tcttgcgcaa cgtggttccg atcggtccgc tgctcggaca
     6421 ggctcccggg ctctttgtgc catgacgctg ttgcaggatg ccaacaaatc ggctgcatgt
     6481 cgccgggtga cagcgcgcag ggcaatggcg gtcttgccag cgctgtgact atcggcctac
     6541 gattcaccgg gagtgtcgtc gccatcatgc cgaccgggac cgttggcgat ggaagaggca
     6601 cgacctgcgg agagaacggg agacgggcct tgaaccaagc gagtccgggc tgcaccctgc
     6661 gcggtgtcgt gctggtcgcc gtgctgctgg ctgcgggcgg gctcggcgcc gataccgcgc
     6721 gcggcgagga tctccgtgag gcgctgaccg ccgcctatct caacaacccg aggctcgcct
     6781 cggggcgcga ccagttgcgg gcgacggacg agctggtgcc gcaggccctg gccgccggtc
     6841 gaccgagcct cttcctcgac ggtcgtgtcg agggtgtgac ctccgatacc gacgttgccg
     6901 agcgtgacaa ccgcctgggc cgtggcgtcg ccctgtcgct gcgccagccg ctctacgcgg
     6961 gcggcgggat cgaggccggc atccgccggg ccgagaacca ggtccgtgcc gaacgggcct
     7021 tcctcctgtc gacccagcag agcgtgctgc tggcttcggt cgatgcctac acggcgacct
     7081 ggcgcgatcg cgccgtcctc gagctcgcgc gcaccaacga gatgcgcctc aggcggcagc
     7141 tcgaggccac ccgcgaccgg ttcgaggtcg gtgaggtggc gcgcaccgac gtcgcccagg
     7201 ccgaggcccg cctgtcccgc gcgcgctccg acgtcgagct cgccaccgcc gatctggccg
     7261 cctccacggc cgcctatcgg gacgtgatcg gcatcgatcc cggcgtgctg gtcgatccgc
     7321 agcccttggg caggctgccg tcgagcgagc cggaggccca ggccctggcg gccgccaatc
     7381 cggacatcac gacggcgagc ttcaatctgc gggccgcccg cgacaatgtc gatgtcgcct
     7441 tcgccgatat cctgccgagc ctcaccctga ccggcgacgt ctcctatgac cagcagccga
     7501 gccaggccct ggattcgagc aacgatgcga ggatcggcct caacctttcg gtgccgctct
     7561 ttcagggcgg cgccgactat tcgcgagtgc ggcagaatcg ccagctggtg agcgccaggc
     7621 gcaacgatct cgagaacacg ctgcgcggcg tccagcgctc ggtgacgacg gcctgggagc
     7681 gggtgatcgc cgcccgtgcc gcgatcgatg cgctggagtc ggaggtccgc gccaacggca
     7741 tcgctctcga gggcgtccag gaggaagccc tggtcggcca gcggaccgtg ctcgacgtgc
     7801 tcgacgccga gaacgagctg ttcgtctcgc aggtcgatct ggtgcgcgcc cggcgcgagt
     7861 gggtgctcgc cacctatcag ctcaagtccg ccatcggcga gctcacggtg gcgcggctcg
     7921 acctgccggt cgagcctttc gacgacgagc gctactacaa ccgcgtgcgc aatcggctgt
     7981 tcggcctgga ataggcccgg ggccactggc cggcgttcgg caattggccg gaaaaacgtc
     8041 gcgcgcttaa tccgtcctta acggcgaaaa gctagggttc cctcaactcg ggcggcactg
     8101 gtgccgttcc gatatttccg atgcgccgta gtgggcgcca cgaaaaggga cccctcgatg
     8161 acctcatccg gcaagcgctg tctcgtcctc gcctccgccc tcgccacgtc cttcgtcgtg
     8221 ccggccctgg ccgacaccga caccgacgtc ctcacggtga tggtcaccgt gcaggaagcc
     8281 tgcgccatca gcggctcgac gctcgacttc ggcacctaca cgggcggcca gcagagcgcg
     8341 ctgaacgggc aagggcagat cagctacacc ggctgcgccg caggcacgct cgtcatcgac
     8401 ttcgacggcg gcaccagcgg cgacgtccag gcccgacgca tggccagcaa tgccggcggc
     8461 acgctcggct accagctcta ccgcaactcc agccgcaccc aggtctgggg caccggcgcc
     8521 aatgcccagc aggtgcagct cctgcaggca ggtgccggca acatcccggt ctatggcagc
     8581 atccccggcg ggcaggccgc cgccgccggc agctacaccg acaccgtcaa tgtcacgttg
     8641 accttctgag cccgggcaac ggacgaccgg cgcgggctcg cgccggtcgc ccccgccggc
     8701 cgggcaatcg cccgtttgcg gcgcctgctg gtcagggcgt ctgttgggcg ctcggctggc
     8761 gggccacggc gtgcggccgg gtcaggtcgt tgcgccgggc ctgcgccgcc atgacgggcg
     8821 acattggcgg ccgctccaca tggcggccgg cgccgcgggt gacgtcgagc tggccgttcc
     8881 gccagaccac cttgccgttg gcgatggtga tcgccggaaa gccctcgacc tcgatgccct
     8941 cgaagacgtt gacgtcgagc ttcgactggt ggctcgcctg ccggatggtg tggcgcgcct
     9001 tcggatccca gaccacgaga tcggcatccg agccggcgcg caccgctccc ttgcgcgggt
     9061 agacattgaa gattttcgcg cagttggtgg aggtcacggc gacgaactcc tcgggcgtca
     9121 ggcggccggc attgacgccg aaatgccaga ggatcttgag ccgctcctcg agcccgcccg
     9181 tgccgttcgg gatcatcgtg aagttgtccc ggcccttggc cttctgctcg gcgaggaagg
     9241 tgcagtggtc ggtcgccgtg acctggagct ggccgttcat gaggccggcc cagagcgcgt
     9301 cctggtgctc cttcgagcgg aagggcgggc tcatcacccg cgaagcggca taggcccagt
     9361 cggggctctg gtagacgctg tcgtcgaggc agagatactg cgccaggggc tcgccgaaga
     9421 cgcgctgccc ctcgctccgt gccctggcaa tggcctccac cgtctcgcgg caggaggtgt
     9481 gcacgatgta gagcggcacg ccggtgatct tggccagcat gatggcgcgg atcgcggcct
     9541 cggcctcgac atgcggcggg cgcgacagcg catggccctc cggcccggtg atgccctggg
     9601 cgagcagcga ctgctgcagg cggaagaccg cctcgccgtt ctcggcatgg acgagcggca
     9661 gggcgccgag ctcgcggcag cgcgagaagc tctggaagag gatctcgtcg tcgaccatca
     9721 aggcgccctt gtacgccatg aagtgcttga agctgttgac gccgcggtcg atgacctcgg
     9781 ccatctgctg ctcgacccgc tcggaccacc aggtgatggc gacgtggaag ccgtagtccg
     9841 ccaccgcctt cgacgcccag tcgctccagt cgtcgagcgc cgccgtcagg ctctgctgcg
     9901 gcgacgggat gcacatgtcg atgatcatgg tggtgccgcc ggcgaggccg gagcaggtgc
     9961 cggaatagaa gtcctcgatc gccaccgtgc ccatgaacgg catctgcatg tgggtgtgcg
    10021 ggtcgatgcc gcccggaatg accagggccc cgccggcgtc gatcacctcg gcgccggtcg
    10081 gcacgtcgag atccggcccg acggcccgga tcgtgccgcc ctcgatcagg acgtcggcgc
    10141 gctcgctctg atcggcattg acgactgtcc caccctggat gacgaggctc atggcgtccc
    10201 tctcttcgtg tccgttgcga cttcccctat ggtcgagcat atcgccgccg cccgggtcct
    10261 gccagagccg atgaccttcg ctgccaccct tcgatgagag cctgctcgcc atgaaagccg
    10321 gcaaccgcac cctcggcctg cgcctgcgcg tcgtgctgga gccggacatc gcgatcggcc
    10381 ccggcaaggc cgatctgctc gagggcatcg gcgagaccgg ctcgatcgcc gccgccggcc
    10441 ggcgcatggg catgagctac aagcgcgcct ggctcctggt cgagacgatg aaccgctgct
    10501 ttcgcgagcc tttggtccgg accagccggg gcggcagcgc caggggcggc gccgtgctga
    10561 ccccggcagg tgccgaggtg ctcgccctct accggcgcat ggaggaacgc tccgcccgcg
    10621 cagtcgaggc ggagctcgct ggcctgcagg cgctctgccg cgaccggacg ccggaagaat
    10681 aacgaaaaca gcgcttgtcg gctggctggc ctcaccgata agtatcgcgg aacatatcgc
    10741 ttcgaaccag gcggaggggg tctcatgcgg gccgtgcagc gcgcagtcgt catcgtcgcc
    10801 gtggccgcgc tgggcctcgc cgggacaggc caggcccggg ccgccgaggt caccgtcttc
    10861 gcggccgcga gcctcaagaa cgcgctcgac gaggtggccg ccggctggca ggccgcgacc
    10921 ggcaacaccg tgtccatctc ctatgccggc agcgcccgcc tcgcccggca gatcgagcag
    10981 ggggcaccgg cggacctgtt catctcggcg aacaccgcct ggatggatca tctcgacgag
    11041 gcggggctga tcgacgaggc gagccggcgc gacctgctcg gcaaccgcct cgtcctcgtg
    11101 gcgcacggcc gggatgtggc gccggtggac ctcgcacccg gcgccgatct cgcccatcgg
    11161 ctcggcgacg gccggctcgc catggccctg gtcgacgcgg tcccggccgg catctacggg
    11221 aaggcggcgc tcgaagaact gggcctctgg gccagcgtcg ccgaccggac agcgcagacc
    11281 gacaacgtgc gcgccgcgct ggcgctggtg gcgctcggcg aagcgccgct cggcatcgtc
    11341 tacgcgaccg acgccgtggc cgaggacggc gtgacggtgg tcgccacctt ccccgaggac
    11401 agccatccgc cgatcgtcta tccggtggcg ctgaccgtcg agggcacccg gtcggcggcg
    11461 gtcgacctcc tggcctggct cggctcggcc gcggcgcgcg ggtcgttcga gcgccagggc
    11521 ttctccgtcc tcgattgagg tgaaccttgc tgcagctttc gcccgaggaa tggacggccg
    11581 tggccctctc gatccgggtc gccggctggg cgacgctgct cagcctgccg ctcggcatcg
    11641 ccacggcgct gctgctcgcc cgcaggcggt tctgggggcg cgagctgctc aatggcctcg
    11701 tccatctgcc gctgatcctg ccgcccgtcg tcaccggcta catgctgctc ctgctgttcg
    11761 gccggcgcgg cccgatcggc agcctgctcg aggagacctt cggcatcgtc ctcgcctttc
    11821 gctggaccgg ggcggtgctc gcctgcgcca tcatggcgtt tcccttgatg gtccgcgcca
    11881 tccgcctcgc cgtcgaggcc gtcgacccga agctcgagca ggcggccggc acgctcggcg
    11941 ccaaccggct ctggaccttc gccaccatca ccctgccgct gatcctgccc ggcgtcatcg
    12001 ccggcgccat cctcgccttc gccaaggcga tgggcgagtt cggcgccacc atcaccttcg
    12061 tctc
//
LOCUS       DAOTFI010000147        12024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6318_length_12024_cov_7.416326, whole genome shotgun sequence.
ACCESSION   DAOTFI010000147 DAOTFI010000000
VERSION     DAOTFI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6318_length_12024_cov_7.416326"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="P5558_15045"
     CDS             complement(<1..143)
                     /locus_tag="P5558_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY25682.1"
                     /translation="MRLKGKRALVTGAGSGFGRAIAETYVREGAKVAILDIDGDAAER
                     VASR"
     gene            complement(250..1686)
                     /locus_tag="P5558_15050"
     CDS             complement(250..1686)
                     /locus_tag="P5558_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY25683.1"
                     /translation="MRHYDCLIDGLEQPAADGRAIEMLCPSDGRAIASVARGGAAEVD
                     AAVGAARRALEAGAYGRLAAVERGRILSRMAAAVTARHAELAELEARDCGKPMAQART
                     DITCLARYLEFYGGAADKVHGLQIPFLGDWLVTGLREPHGVTAHIIPWNYPAQIFGRS
                     VGAALAMGNACVLKPAEDAGLTCLEVARLALECGLPPGALNVVTGYGEEAGAALSQHG
                     DIDFISFTGSPEIGTAIQTAAARRTIGCTLELGGKSAQLVFADADLDKALPIIVNAII
                     QNAGQTCSAGSRVLIEATVFDEVAARLAERFSALRVGTHAMDLDLGPLINRQQQARVQ
                     RFVERAADLPLLAEGRLAPDLPAGGHYAVPRLYGPVPADHALAQEEVFGPVLVAMPFQ
                     DEAEAVRLANGTAFGLVAGVWTRDGGRQMRVARRLRAGQVYVNGYGAGGGIELPFGGV
                     KRSGHGREKGFEALYEFSQLKTIAFNHG"
     gene            1767..2891
                     /locus_tag="P5558_15055"
     CDS             1767..2891
                     /locus_tag="P5558_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRY25684.1"
                     /translation="MTRDLEGLLVVSVEQAVAAPYASGRLADAGARVIKVERPEGDFA
                     RRYDRYVNGQSAYFVWLNRGKQSIALDLKAADDLALLQRLIGRADVVIQNLAPGAADR
                     LGVGPLAMRAANPGLIYCSISGYGEQGPYARQKAYDLLVQAESGLASINGTAEGPARV
                     GVSVCDIAAGMTAFQAILQALIGRGRTGEGRAIEVSLYHAMADWMNVPYLQHRYGGHT
                     PARAGLSHPTIAPYGAFICGDGKAVLLSIQNEREWQSLCTHVLGNAGLAVEPRFETNP
                     ERVANRMELDRLIKQAFAPFDRETVIERLDAAGIAYGRLSTLDDLEAHPQNRYLLVET
                     PASPVELLAPGAAVTDEDLAVGPVPGLDEHGPVIRAEFAL"
     gene            2907..3434
                     /locus_tag="P5558_15060"
     CDS             2907..3434
                     /locus_tag="P5558_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25685.1"
                     /translation="MADFFALIDTYRLGDLASLLGLVGLTLTVWAAWRSKTAAESAAD
                     AARQAREHILTFHSTTSLASVIFRMEALKVLHRAGRIDHMPERYSALAGDLMAIRSRN
                     PSLTAREQAIIQQSVLLFRGMEYEIDLAAQDGERRVDIAHLNRAISLAFDELTVLLHD
                     LENRAQEARQDDRTH"
     gene            3418..3726
                     /locus_tag="P5558_15065"
     CDS             3418..3726
                     /locus_tag="P5558_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25686.1"
                     /translation="MTELTESLVERLLMKTKEGEISWRESVRPNEYTVSFPSSSVGIV
                     DDKDGYRLRLYNDTGMLIDETRIKRAPALSQMMELWGLARRQAWRVDETLQELLKELA
                     "
     gene            3753..4529
                     /locus_tag="P5558_15070"
     CDS             3753..4529
                     /locus_tag="P5558_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY25687.1"
                     /translation="MRFSGRTVIVTGAGRGIGRAAAEAFAAEGAAVVVADIDAEGGNG
                     AVAAITAAGGSALFVPCDVGDAVQCGSLVDATLEWAGRLDVLVNNAAVLRTADFLELT
                     EADFDTVLRVNLKGAFLMGQAAARAMVGRKSPGAIVNMSSINATVAIPSQVPYVVSKG
                     GINQLTRVMALALADKGIRVNAVAPGSIMTDMLRTVMTDDTARRTILSRTPLGRAGEP
                     VEIARLVLFLASDEASYITGEVVTADGGRMALNYTVPVTD"
     gene            4625..5254
                     /locus_tag="P5558_15075"
     CDS             4625..5254
                     /locus_tag="P5558_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HRY25688.1"
                     /translation="MDKPLMPKATAVWLIDNTGLTFDQIADFCGLHPLEVKGIADGEV
                     GAGIRGLDPIGQGMVTREELERCQKDPKLRLKIARSVAADVKPPKRREPRYTPLSKRQ
                     DRPDGIAWLLRHHPELGDQQICKLLGTTKNTVEAVRERTHWKSQEIRPRDPVLLGLCQ
                     QIELDDAIARAKADAEKLAERAASKAAAARPAEAKAPVIQEPVEAGEAE"
     gene            complement(5266..5811)
                     /locus_tag="P5558_15080"
     CDS             complement(5266..5811)
                     /locus_tag="P5558_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25689.1"
                     /translation="MTQERYPGEQHGLGLFLEELQPVVARELGQDSLLHHAIRRALAT
                     GRLTHLRHARGVFNSLPRDLRQTLQSGIVATPERAPKRERLLDTYGRREPQPFVAIDC
                     EIGHDASPEPQIGLRHELLDDVALRVLVRPGSLPSGVAGRLREIADLIDQDRRLLSAR
                     YWRAARMRVVTGDGCENAESG"
     gene            complement(6033..7208)
                     /locus_tag="P5558_15085"
     CDS             complement(6033..7208)
                     /locus_tag="P5558_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRY25690.1"
                     /translation="MPIVNRIAEYSDTLTAWRRHLHSHPELGYEEVETAAFVAARLRE
                     MGVDEVHEGIGRTGVVGVVRGGTGGRRIGLRADMDALPIEEATGLPHASTRPGKMHAC
                     GHDGHTTMLLGAAKYLAETRNFDGTVCLVFQPAEEGRAGARAMLEDGLLERFPVEEIY
                     GLHNWPSLPVGEFAMCTGPAMAASDEFRITIKGRGCHAAMPNLGLDPVFVGAQIVIGL
                     QGIVSRETDPVDNAVLSVTRFHAGQAFNVVPETAELVGTVRTFEPGTRDRIERRLGEV
                     AQGIARSFGLDADIAYRRGYPPTVNHAREAVMGADAAALVVGEERVDRTPRPVMGAED
                     FAFMLEAVPGSYIWMGSAAGADSPPLHSAHYDFNDEALPLGVSYWAKLVESRLPRAG"
     gene            7334..8533
                     /locus_tag="P5558_15090"
     CDS             7334..8533
                     /locus_tag="P5558_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25691.1"
                     /translation="MAKSVRHVAALATLGSFLMAGTALAEGAKIGALIPLTGGLQSYG
                     EASLDGMMLAIDEVNAAGGVLGGPVEVIVGDTQTKAQAAIDAAKKMVSVDGVAGILGA
                     LSSGNTIPVATSVSAVDQVPQISNASTAPAITTLEDNDFLFRTVPSDAYQGAVLAGLT
                     DAAGIGSVAILYINNDYGVGLAESFAAGYGGQITSSSAFEPNKASYRGELQAAAEGDP
                     EALLLIAYPDDGGLLILRQSLEEGFFDRFVVTDGLKTAQLVEDIGAEYMEGVFGTAAG
                     AVDNDASAHFAAAYEAKHGEMPPLPYIDSGFDAAMILALAIEKAGSTDGPAIRDAMRE
                     VTNAPGEPVGPGDFAKAKELIAAGTDIDYQGAAGNHEFDENGDVTGIFEHWAVKDGTI
                     VSVEMIE"
     gene            complement(8589..8777)
                     /locus_tag="P5558_15095"
     CDS             complement(8589..8777)
                     /locus_tag="P5558_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25692.1"
                     /translation="MTRDDDDPAHASTARATAAKKPVPVRRPLRERIAELAADYRRHP
                     LSGLPADKAFFDGLSDNG"
     gene            complement(8841..9788)
                     /locus_tag="P5558_15100"
     CDS             complement(8841..9788)
                     /locus_tag="P5558_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY25693.1"
                     /translation="MSLVGLASYLVFFLTLAGIYALMAQGLNVQWGYAGMLNVGVAAF
                     FAIGAYTSAILTTGASDGHLGGFGLPWPLGFIAAMAVAGLLGLGIGIVTLKLRSDYLA
                     IATIGIAEIVRLVLKNEEWLTGGVRGIAGIPRPLDGLIAGQNALLLLALIVALILVVH
                     WALERAHRSPWGRVLRGIRDNETAARAMGKDSLVYRMQAFVMGCAIMGLAGAAYAHFV
                     GFISPEAFDPVFATFIVWVMLIAGGSGNNKGAILGAVVIWAVWSGTQFLTGHFPAQYQ
                     TQAGALRVLMIGVLLQVILIFRPEGLLPEKPPAPIWRKE"
     gene            complement(9790..10653)
                     /locus_tag="P5558_15105"
     CDS             complement(9790..10653)
                     /locus_tag="P5558_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY25694.1"
                     /translation="MIEILQLLLYGTLLGSIIALGAVGVTLTFGILGFANFAHGDLMA
                     FGAYVCFLLYTQLAMPFWLGLVAAVPATILLAVVVDRVLYRRFRASRPIISLISSFGA
                     ALIIRSSIQIIWGPGTWLYSSKLVMAQRFEGIRYKPDQLLIIAATIVVVIALHLFLQR
                     TRVGKAMRAMADNATLARLSGIDTERVILWTWVIGGGLAALAGVFLGMDTRLQPEMGW
                     FLLLACFAAAILGGIGKPMGAIAGGYIIGMMMELSTLVLPAEYSPVVPFAIMVLLLLV
                     RPTGLFAGRST"
     gene            complement(10658..11362)
                     /locus_tag="P5558_15110"
     CDS             complement(10658..11362)
                     /locus_tag="P5558_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25695.1"
                     /translation="MSLLAVTGLRGGYGDVDILHGVSLAVEPGQIVVIVGPNGAGKST
                     ALKAVFGLVRIRGGSIVFADRDLTGRRPDQIVEAGISFVPQTENVFPTLTVEENLDMG
                     AYLRRDDIRPQRERVYELFPPLREKRRQAAGTLSGGQRQMVAMGRALMLDPKLLLVDE
                     PTAGLSPLFMNQIFEILVDINKTGVAIMMVEQNAKQALGIADRAYVLAMGENRHEGTG
                     AELLGDPEVATMFLGG"
     gene            complement(11359..>12024)
                     /locus_tag="P5558_15115"
     CDS             complement(11359..>12024)
                     /locus_tag="P5558_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25696.1"
                     /translation="PNGAGKTTLFNMIAGAFPPTAGGIHFQGEDVTGLPAHRLFHKGI
                     VRTFQIPHEFGRMTCLENLMLVPPGQSGERLWGAWLGWGRVRDEETAIRRKALDVLDF
                     LKMSHLRDKLASDVSGGQKKLLELGRTMMTKATLVLLDEPGAGVNRTLLAEIAHAIRT
                     LNEERGYTFCIIEHDMGFIAELCDPVICMAEGRVLCAGTMAEVRRDPRVLEAYLGAAG
                     EAA"
BASE COUNT         1962 a         4201 c         4103 g         1758 t
ORIGIN      
        1 cgcgaggcca cccgctcggc cgcgtcgcca tcgatgtcga ggatggcgac cttcgccccc
       61 tcgcgcacat aggtctcggc gatcgcccgc ccgaaccccg acccggcgcc cgtaacgagc
      121 gcccgtttcc ccttgagccg catggtctcg ttctccctgt tgctggccgg cctcgtagcc
      181 gtcggaacgc cagtcgtcga taccgctgtc gcgtcggctt attgaagtgc ccgtacccgg
      241 tccgtcagcc tagccgtgat tgaaagcgat cgtcttgagc tggctgaact cgtagagcgc
      301 ctcgaagccc ttctcgcggc cgtggccgga gcgcttgacg ccgccgaagg gcagctcgat
      361 gccgccgccg gcgccgtagc cgttgacata gacctgcccc gcccggagcc gccgcgccac
      421 gcgcatctgc cggccgccgt ccctggtcca gaccccggcg acgagtccga aggcggtgcc
      481 gttggcgagc cgcaccgcct cggcctcgtc ctggaacggc atggccacca gcaccggccc
      541 gaagacctcc tcctgggcca gcgcgtgatc cgccggcacg gggccgtaga gccgcggcac
      601 cgcgtagtgg ccgcctgccg gcaggtccgg cgccaagcga ccctcggcga ggagcggcag
      661 gtcggcggcg cgctcgacga agcgctgcac gcgcgcctgc tgctgccggt tgatcagggg
      721 gccgaggtcg agatccatgg cgtgggttcc gacccgaagg gcgctgaacc gctcggcgag
      781 gcgggccgcc acttcgtcga agaccgtagc ctcgatcagc acccggctgc cggccgagca
      841 ggtctggccg gcattctgga tgatggcgtt gacgatgatc ggcaacgcct tgtcgagatc
      901 ggcgtcggcg aagacgagct gcgccgactt gccgccgagc tcgagcgtgc agccgatggt
      961 gcgccgcgcc gccgcggtct ggatggccgt gccgatctcg gggctgccgg tgaacgagat
     1021 gaagtcgata tcgccgtgct ggctcagcgc cgcgcccgcc tcctcgccgt agccggtgac
     1081 cacattgagg gcacccggcg gcaggccgca ctccagcgcc agccgcgcca cctcgaggca
     1141 ggtgagcccg gcatcctcgg ccggcttcag gacgcaggca ttgcccatgg cgagcgccgc
     1201 acccaccgac cgcccgaaga tctgcgccgg gtagttccag gggatgatgt gcgcggtgac
     1261 gccatgcggc tcgcgcaggc cggtcaccag ccagtcgccg aggaacggga tctggagccc
     1321 gtgcaccttg tcggccgccc cgccatagaa ctcgagatag cgggcgaggc aggtgatgtc
     1381 ggtcctcgcc tgcgccatgg gcttgccgca atcacgggcc tcgagctcgg cgagctcggc
     1441 atggcgggcg gtcacggccg ccgccatccg cgacaggatt cgtccgcgct cgaccgcggc
     1501 gagccggcca taggcgcccg cctcgagggc ccgccgggcg gccccgacgg cagcgtcgac
     1561 ctccgccgcc ccgccgcgcg ccacgctggc gatggcgcgg ccgtccgacg ggcagagcat
     1621 ctcgatggcg cggccgtcgg cggccggctg ctccaggccg tcgatcaggc agtcgtagtg
     1681 gcgcatcaga gcctcccctc cgcatggctt gcgcaatcga caccgttgtg gctccaaggc
     1741 ggcttccgag caaggagccg gccgtcatga ccagggatct cgagggcctc ctcgtggtga
     1801 gcgtcgagca ggcggtggcc gccccctatg ccagcggccg gctcgccgat gccggcgccc
     1861 gggtgatcaa ggtggagcgc ccggagggcg acttcgcccg ccgctacgac cgctacgtca
     1921 atggccagag cgcctatttc gtctggctca accggggcaa gcagtcgatc gccctcgacc
     1981 tcaaggcggc ggacgacctg gccttgctgc agcggctgat cggccgggcc gacgtggtca
     2041 tccagaacct ggcgccgggt gcggccgacc ggctcggcgt cggtcccctg gcgatgcgcg
     2101 ccgccaatcc ggggctgatc tactgctcca tctccggcta tggcgagcag ggcccctacg
     2161 cgcgacagaa ggcctacgac ctcctggtgc aggcggagag cggcctcgcc tcgatcaacg
     2221 gcacggccga gggccccgct cgcgtcgggg tctccgtctg cgacatcgcc gccggcatga
     2281 ccgccttcca ggcgatcctc caggcgctga tcggccgcgg ccggacgggc gagggccggg
     2341 ccatcgaggt ctcgctctac catgccatgg ccgactggat gaacgtcccc tacctgcagc
     2401 accgctatgg cggccacacg ccggcccgcg ccgggctcag ccacccgacc atcgccccct
     2461 acggcgcctt catctgcggc gacggcaagg cggtcctgct ctcgatccag aacgagcgcg
     2521 aatggcagag cctctgcacc catgtgctgg gcaatgccgg gctcgccgtc gagccgcgct
     2581 tcgagaccaa tcccgaacgc gtcgccaacc ggatggagct cgaccggctg atcaagcagg
     2641 ctttcgcccc gttcgaccgc gagacggtga tcgagaggct cgacgcggcg ggcatcgcct
     2701 atggccggct gagcacgctc gacgacctcg aggcgcaccc gcagaaccgc tacctcctgg
     2761 tcgagacgcc ggccagcccg gtcgagctcc tggctcccgg cgctgccgtc accgacgagg
     2821 atctcgccgt cggccccgtc cccggcctcg acgagcacgg ccccgtcatc cgcgcggagt
     2881 ttgcgctttg agccgagacg cgctggatgg ctgatttctt cgcgctgatc gatacctacc
     2941 gtctcggcga tctggcgagc ctgctcggac tggtgggcct gacattgacc gtgtgggctg
     3001 catggcgctc gaaaacggcg gctgaatcgg ctgccgacgc ggcacggcaa gcgcgcgagc
     3061 acattcttac ctttcactcc acgaccagct tggccagtgt tatctttcgc atggaagcgc
     3121 tgaaagtgct gcatcgcgcc ggccgcatcg accacatgcc ggagcgctac agcgcattgg
     3181 cgggtgacct gatggcgatc cgctcgcgca acccgtcgct aaccgccagg gaacaggcta
     3241 tcattcagca atcggttttg ttgttcagag gcatggaata cgagatcgat ctggcggctc
     3301 aggacggaga gagacgtgtc gacattgcac acctcaatcg cgccatatcg ctcgcttttg
     3361 acgagctgac cgtcctgctg cacgatctcg aaaatcgcgc tcaagaggct aggcaagatg
     3421 accgaactca ctgaatctct tgtcgaacga ctcctcatga agaccaagga aggtgaaatc
     3481 agttggcgcg aatccgtgcg accgaacgag tacactgtga gctttcccag ctcgtccgtc
     3541 ggaattgtcg acgataagga cggatatcgt ttacgccttt acaacgatac cggaatgctc
     3601 attgatgaga cacggatcaa gcgagcgcct gctttatcgc agatgatgga gctctgggga
     3661 ttggcgcgac gccaagcatg gcgcgtggac gaaacattgc aagagttgct caaagagctg
     3721 gcctgatcct caccgaggag ggactcgacg aagtgcgctt ttccggcaga accgtgatcg
     3781 tcaccggggc cggccgtggc atcggccggg ccgcggccga ggccttcgcc gccgaagggg
     3841 cggccgtcgt ggtcgccgac atcgatgccg agggcggcaa cggtgcggtc gccgccatca
     3901 ccgccgccgg cggctcggcg ctcttcgtgc cctgcgacgt cggcgacgcc gtgcagtgcg
     3961 gctcgctggt cgacgcgacg ctcgaatggg ccggccggct cgacgtgctc gtgaacaacg
     4021 cggccgtgct ccggacggcg gacttcctcg agctcaccga ggccgatttc gacaccgtgc
     4081 tgcgcgtcaa cctcaagggc gccttcctga tgggccaggc cgcggcgcgc gccatggtcg
     4141 gccgcaagtc gcccggggcg atcgtcaaca tgtcgtcgat caacgccacc gtcgccattc
     4201 cgagccaggt gccctacgtc gtcagcaagg gcggcatcaa ccagctcacc cgcgtcatgg
     4261 cgctcgccct cgccgacaag gggatccggg tcaatgcggt ggccccgggc tcgatcatga
     4321 ccgacatgct gcgcaccgtg atgaccgacg acacggcgag gcgcaccatc ctctcgcgca
     4381 ccccgctcgg ccgggcaggc gagccggtgg agatcgcccg cctcgttctc ttcctcgcca
     4441 gcgacgaggc gagctacatc acgggcgagg tggtgaccgc cgatggcggg cgaatggcgc
     4501 tgaactacac ggtgccggtg accgactgag ccgaccgctg gtgccacacg cctgttgacg
     4561 ggcggcggct aaggccctac ccgcaggcgt tcatccgatt ttgcatgtta ccgaggagac
     4621 tggcatggac aagccgctca tgccgaaggc cacggctgtt tggctcatcg acaacacggg
     4681 tctgaccttc gaccagatcg ccgatttctg cggcctgcat ccgctcgagg tgaagggcat
     4741 cgccgatggc gaggtggggg ccggcattcg cggcctcgac cccatcggcc agggcatggt
     4801 gacgcgcgag gagctcgagc gttgccagaa ggaccccaag ctgcgcctca agatcgcgag
     4861 gtcggtggcc gccgacgtca agccgccgaa gcgccgcgag ccccgctaca ccccgctctc
     4921 caagcggcag gaccgaccgg acggcatcgc ctggctgctc cggcaccatc ccgagctggg
     4981 cgaccagcag atctgcaagc tgctcggcac gaccaagaac acggtcgagg cggtgcgcga
     5041 gcgcacccac tggaagagcc aggagatccg cccgcgcgac ccggtgctgc tcgggctctg
     5101 ccagcagatc gagctggacg atgcgatcgc gcgggccaag gcggacgccg agaagctcgc
     5161 cgagcgggcc gcgagcaagg ccgccgctgc acggccggcc gaagccaagg ccccggtcat
     5221 ccaggaaccc gtggaagccg gcgaagccga gtagccgggg cggcgctagc cgctttcggc
     5281 attctcgcag ccgtcgccgg tgaccacccg catacgggcg gcacgccagt agcgcgccga
     5341 gaggagccga cggtcctggt cgatgaggtc ggcgatctcg cgcagccggc ccgccacgcc
     5401 ggacggcagc gagccggggc ggaccagcac acgcagcgcc acgtcgtcga gcagctcgtg
     5461 acggagcccg atctgcggct cgggcgaggc atcgtggccg atctcgcagt cgatggcgac
     5521 aaagggctgc ggctcccgcc ggccataggt gtcgagcagc cgctcgcgct tgggggcgcg
     5581 ctccggcgtc gcgacgatgc cggactgcag cgtctgccgc aggtcgcggg gcaggctgtt
     5641 gaagaccccc cgcgcatgcc gcagatgcgt cagccggccg gtggccaggg cgcggcggat
     5701 ggcatgatgg agcaggctgt cctgaccgag ctcgcgggcg accacgggct gcagctcctc
     5761 gaggaagagc ccgaggccat gctgctcgcc gggatagcgt tcttgcgtca tgaggctgcc
     5821 gttcccgtca acacctggcc agtcaacagc gggccggtcg atcccgagcc gcgccggccg
     5881 caaccggcgc cgaccggagg gatgctccga cccttgctta ccagagttta acgtatgtgg
     5941 cggggcgctc aatcgtcaaa catggttgcc gaacatctca tgggatgcat cgggctagtg
     6001 gacattcgcg ttagggttaa tcctcgccac cttcagcccg cgcgtggcaa ccggctctcc
     6061 accagcttcg cccagtagct cacgccgagc ggcagcgcct cgtcgttgaa gtcgtaatgc
     6121 gcgctgtgca aaggcggcga gtcggctccg gcggcgctgc ccatccagat gtagctgccg
     6181 ggcacggcct cgagcatgaa ggcgaaatcc tccgccccca tcaccgggcg cggtgtccga
     6241 tcgacccgct cctcgcccac caccagggcc gccgcatcgg cgcccatgac ggcctcgcgc
     6301 gcatggttga ccgtcggcgg gtagccccgg cgataggcga tgtcggcgtc gagcccgaag
     6361 ctccgggcga tgccctgcgc cacctcgccg agccgccgct cgatccggtc gcgcgtcccg
     6421 ggctcgaagg tcctgaccgt gccgacgagc tcggccgtct cgggcacgac gttgaaggcc
     6481 tggccggcat ggaagcgggt gaccgagagc accgcgttgt cgacgggatc ggtctcgcgg
     6541 ctgacgatcc cctgcaggcc gatcacgatc tgcgcgccga cgaagaccgg gtcgagcccc
     6601 aggttcggca tcgccgcgtg gcagccgcga cccttgatgg tgatgcggaa ctcgtccgag
     6661 gcggccatcg ccgggccggt gcacatggcg aactcgccga ccggcagcga gggccagttg
     6721 tgcaggccgt agatctcctc gaccgggaac cgctcgagca gaccgtcctc gagcatcgcc
     6781 ctggccccgg cgcggccctc ctcggccggc tggaagacga ggcagacggt gccgtcgaaa
     6841 ttgcgggtct cggccaggta cttggcggca cccagcagca tggtggtgtg cccgtcatgg
     6901 ccgcaggcgt gcatcttgcc gggccgggtc gaggcatgcg gcaggccggt cgcctcctcg
     6961 atcggcagcg cgtccatgtc ggcgcgcagg ccgatgcgcc ggccgcccgt gccgccccgc
     7021 accacgccga ccacgccggt ccggccgatc ccctcgtgca cctcgtcgac gcccatctcg
     7081 cgcagccggg cggcgacgaa ggccgccgtc tccacctcct cgtagccgag ctccgggtgg
     7141 ctgtgcaggt gacggcgcca ggcggtcagg gtgtcgctgt actcggcgat acggttgacg
     7201 atcggcatga tggctcccag ttgcgccggt cggggcaagt tcctaagaca gtagacgaat
     7261 gtgcggcggc gggggcgggc tggcaagcag caccacgccg gaccgccgac aaaccggctc
     7321 aaggaggttc gcaatggcca aatcggttcg gcatgtcgca gcgctcgcca cgctcggctc
     7381 cttcctcatg gcgggcacgg cgctcgccga gggtgccaag atcggtgccc tgatcccgct
     7441 gaccggcggg ctgcagtcct acggcgaggc gtcgctcgac ggcatgatgc tggccatcga
     7501 cgaggtcaat gccgccggcg gcgtgctcgg cgggccggtc gaggtgatcg tcggcgatac
     7561 ccagaccaag gcgcaggcgg cgatcgacgc ggccaagaag atggtctcgg tcgacggcgt
     7621 ggccggcatc ctcggcgccc tctcctcggg caacaccatc ccggtcgcca ccagcgtctc
     7681 ggcggtcgac caggtcccgc agatctccaa tgcatcgacc gccccggcga tcaccacgct
     7741 cgaggacaac gacttcctct tccgcacggt cccgtccgac gcctatcagg gtgcggtgct
     7801 cgccgggctc accgacgccg ccggcatcgg cagcgtcgcc atcctctaca tcaacaacga
     7861 ctacggcgtc ggcctcgccg agagcttcgc cgccggctat ggcggccaga tcacctcgtc
     7921 ctcggccttc gagccgaaca aggcctccta tcgcggcgag ctgcaggccg ccgcggaagg
     7981 cgaccccgag gcgctcctgc tcatcgccta ccccgacgat ggcggcctgc tcatcctccg
     8041 ccagtcgctg gaggagggct tcttcgaccg cttcgtcgtc accgacggcc tcaagacggc
     8101 ccagctcgtc gaagacatcg gtgccgagta catggaaggt gtcttcggca cggctgccgg
     8161 tgcggtggac aacgacgcca gcgcccactt cgccgccgcc tacgaggcca agcacggcga
     8221 gatgccgccc ctgccctata tcgacagcgg cttcgacgcg gcgatgatcc tggcgctggc
     8281 catcgagaag gccggcagca ccgacggccc ggccatccgc gacgcgatgc gcgaggtgac
     8341 caacgccccg ggcgagccgg tcggccccgg cgacttcgcc aaggccaagg agctgatcgc
     8401 cgccggcacc gacatcgact accagggtgc ggccggcaac cacgagttcg acgagaacgg
     8461 cgacgtcacc ggcatcttcg agcactgggc ggtcaaggac ggcaccatcg tctcggtcga
     8521 aatgatcgag tagtggcacc ggccgccgcc cgtatcctgg tcacgggcgg cggcctcggt
     8581 gacgaatttc agccgttgtc gctgagcccg tcgaagaaag ccttgtcggc agggagcccg
     8641 ctaagcggat gtcggcgata gtccgccgcc agttcggcga tgcgctccct gagcgggcgc
     8701 cgaactggca ccggtttctt cgcggccgtt gcacgtgccg tgcttgcatg ggctggatcg
     8761 tcgtcgtcac gggtcatctg cgctgctccg gatcatgacc ggaatcgagc ccaactccgc
     8821 cgcgaacgca acgccccacc ctactccttg cgccagatcg gcgccggcgg cttctcgggc
     8881 aggaggccct cggggcggaa gatcaggatc acctggagca gcacgccgat catcaggacg
     8941 cggagcgctc ccgcctgggt ctggtactgg gccgggaagt ggccggtcag gaactgggtg
     9001 cccgaccaga ccgcccagat gaccacggcg cccaggatcg cacccttgtt gttgccgctg
     9061 ccgccggcga tcagcatcac ccagacgatg aaggtggcga acaccggatc gaacgcctcg
     9121 gggctgatga agccgacgaa atgcgcgtac gccgccccgg cgaggcccat gatggcgcag
     9181 cccatgacga aggcctgcat gcggtagacg aggctgtcct tgcccatcgc ccgggccgcc
     9241 gtctcgttgt cgcggatgcc gcgcagcacc cggccccagg gcgagcggtg cgcccgttcg
     9301 agcgcccagt gcacgaccag gatcagggcg acaatgagcg ccagcaggag cagcgcgttc
     9361 tggccggcga tcagcccgtc gagcggccgc gggatgccgg cgatgccgcg caccccgccg
     9421 gtcagccatt cctcgttctt gagcacgagc cggacgatct cggcgatgcc gatcgtcgcg
     9481 atggcgagat agtccgagcg caatttgagc gtgacgatgc cgatgccgag cccgagcagc
     9541 cccgccaccg ccatggcggc gatgaagccg agcggccagg gcaggccgaa gccgcccaga
     9601 tgcccgtccg aggcacccgt ggtcaggatg gcgctggtat aagcaccgat cgcgaagaaa
     9661 gcggcgacgc cgacgttcag catgccggca tagccccact gcacgttgag gccctgggcc
     9721 atcagcgcgt agatgccggc cagcgtcagg aagaagacga gataactggc gagaccgacg
     9781 aggctcatgt caggtgctcc gtccggcgaa gagcccggtc gggcggacga gcagcagcag
     9841 gaccatgatg gcgaacggca cgaccggtga gtactcggcc ggcaggacca gcgtgctcag
     9901 ctccatcatc atgccgatga tgtagccgcc ggcgatggct cccatcggct tgccgatgcc
     9961 gccgaggatg gcggcggcga agcaggcgag cagcaggaac cagcccatct ccggctgcag
    10021 ccgggtgtcc atgccgagaa agaccccggc cagggccgcg aggccgccgc cgatcaccca
    10081 ggtccagagg atgacccgct cggtgtcgat gccggagagc cgggccagcg tggcattgtc
    10141 ggccatcgcc cgcattgcct tgccgacccg ggtgcgctgc aggaagagat ggagcgctat
    10201 gaccaccaca atggtggccg cgataatgag aagctggtcc ggcttgtagc ggatgccctc
    10261 gaagcgctgc gccatgacca gcttgctgga atagagccag gtgcccgggc cccagatgat
    10321 ctggatcgag gagcggatga tcagggcggc gccgaaggac gagatcaggc tgatgatcgg
    10381 ccggctcgcg cgaaagcgcc gatagagcac ccggtcgacc accaccgcca gcaggatggt
    10441 cgccggcacg gccgccacca gcccgagcca gaacggcatg gcgagctgcg tgtagagcaa
    10501 aaagcagaca taggcgccga acgccatcag gtcgccatgc gcgaaattgg cgaagccgag
    10561 gatgccgaag gtgagcgtca cgcccacggc acccagcgcg atgatcgagc caaggagcgt
    10621 gccgtagaga aggagctgca ggatttcgat catgcgatca accgccgaga aacatggtgg
    10681 ccacctcggg gtcgccgagc agctcggcac ctgtgccctc gtgccggttc tcgcccatcg
    10741 ccagcacata ggcgcggtcg gcgatgccca aggcctgctt ggcgttctgc tcgaccatca
    10801 tgatggcgac gccggtcttg ttgatgtcga ccaggatctc gaagatctgg ttcatgaaga
    10861 gcggcgagag cccggccgtc ggctcgtcga ccaggaggag cttcgggtcg agcatgaggg
    10921 cccggcccat cgccaccatc tgccgctgcc cgccggagag ggtgccggcc gcctgccgcc
    10981 gcttctcgcg caaaggcggg aagagctcgt agacgcgctc gcgctgcggg cggatgtcgt
    11041 ccctgcggag atatgccccc atgtcgaggt tctcctcgac ggtcagggtc ggaaagacgt
    11101 tctcggtctg cggcacgaag gagatgccgg cctcgacgat ctggtcgggc cggcggccgg
    11161 tgaggtcacg gtcggcgaag acgatgctgc cgccacggat cctgaccagc ccgaagacgg
    11221 ccttgagcgc cgtcgacttg ccggcgccat tggggccgac gatcaccacg atctggcccg
    11281 gctccacggc gagcgagaca ccgtgcagga tgtcgacgtc gccgtagccg cccctgagcc
    11341 cggtcacggc gagcaggctc aagcggcttc tcccgcagca ccgagatagg cctccagcac
    11401 ccggggatcg cgccgcacct cggccatcgt gccagcgcag agcacccggc cctcggccat
    11461 gcagatcacc gggtcgcaga gctcggcgat gaagcccatg tcgtgctcga tgatgcagaa
    11521 ggtgtagccg cgctcctcgt tcagcgtgcg gatggcatgg gcgatctcgg cgagcagcgt
    11581 gcggttgacg ccggccccgg gctcgtcgag cagcaccagc gtcgccttgg tcatcatcgt
    11641 ccgaccgagc tcgagcagct tcttctgccc gcccgagacg tcgctcgcga gcttgtccct
    11701 gagatgcgac atcttgagga agtcgagcac gtcgagcgcc ttgcggcgga tcgccgtctc
    11761 ctcgtcgcgc acccggcccc agccgagcca ggcgccccag agccgctcgc cgctctgacc
    11821 gggcggcacc agcatcaggt tctcgaggca ggtcatccgg ccgaactcgt gcggaatctg
    11881 gaaggtgcgg acgatgccct tgtggaagag ccggtgcgcc ggcaggccgg tgacgtcctc
    11941 gccctggaaa tgaatgccgc cggccgtcgg cgggaaggcg ccggcgatca tgttgaagag
    12001 cgtggtcttg ccggcgccgt tcgg
//
LOCUS       DAOTFI010000148        11942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6376_length_11942_cov_6.757466, whole genome shotgun sequence.
ACCESSION   DAOTFI010000148 DAOTFI010000000
VERSION     DAOTFI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11942
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6376_length_11942_cov_6.757466"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..594)
                     /locus_tag="P5558_15120"
     CDS             complement(331..594)
                     /locus_tag="P5558_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HRY25697.1"
                     /translation="MAGGWTRDGAVQEQIDDTVSDAVRLARERRQAGEGATHCDRCGE
                     EIPEARRRALPHARTCVPCQSALDDRPGAALYNRRGSKDSQLR"
     gene            1103..1336
                     /locus_tag="P5558_15125"
     CDS             1103..1336
                     /locus_tag="P5558_15125"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRY25698.1"
                     /translation="MAARLVDRIVADPRVMMGKPVVKGTRIPVELILRRLGAGDGDAQ
                     ILADYPTLSIEDIRAAQAFAADHIAGEDIIAAE"
     gene            1337..1717
                     /locus_tag="P5558_15130"
     CDS             1337..1717
                     /locus_tag="P5558_15130"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRY25699.1"
                     /translation="MKLLADECVAGSTVAAMRAAGFDVEWIAQTLPGAADADVLARAF
                     GAGRLLLTEDKDFGELTIRFGHAFHGVVLVALAGLPAAARAARTVAALQALGDRGAGQ
                     FVVIEQHRIRRRALGAPGKPERPR"
     gene            complement(1960..3267)
                     /locus_tag="P5558_15135"
     CDS             complement(1960..3267)
                     /locus_tag="P5558_15135"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY25700.1"
                     /translation="MGALALFSTTSVSAQPLPMAEAESVGMSSERLDRIGQVFQQEID
                     SGALPGAVVMVARDGKLVYSAVLGMEDPQAGEPMQEDSIFRLYSMTKPLVSVAAMILM
                     EEGKLHLAEPVAKYLPEFADLEVSVPATDGDGKTTYSNVPLKRPMTVQDLLRHTAGLA
                     YGELTRNPEVQQAYAEAGVFNPGGMPFDARGVAPDEQVAGLGRAQLVNQPGEVWQYGL
                     ATDVLGRVIERISGMELGAFLEARLFGPLKMDSTGFYVPENAMERLAESFATNAAGAP
                     IALIDVSAPPANASGGGGAVSTAGDYLRFNQMLLQGGVLEGVQVLSPTSVKLMTADHL
                     GERIQTPLTPGEPMMGVHGYTFGLGFMVREEDGIAGVPGSQGEYMWAGYAGTFFWVDP
                     EEKLTAVLMAQMPGPTAAHYRRLVKQLVYQAIIDAGKDRPLIQ"
     gene            complement(3628..3909)
                     /locus_tag="P5558_15140"
     CDS             complement(3628..3909)
                     /locus_tag="P5558_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRY25701.1"
                     /translation="MLMDIVEARHLGQHRLFLRFEDGVEGEVDIAGIVGFTGILTPLA
                     GTAFFAKVVADTETDTVTWPNGADLDPDVLHAALTGVPVAHSLNLADAS"
     gene            complement(4067..5743)
                     /gene="ettA"
                     /locus_tag="P5558_15145"
     CDS             complement(4067..5743)
                     /gene="ettA"
                     /locus_tag="P5558_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRY25702.1"
                     /translation="MASYQYIYVMKRLTKIFPGGKKLFENITLAFLPGAKIGVLGVNG
                     SGKSSLLKIMAGRDQDFAGEAWAADGVKIGFLEQEPHLDETKDVMGNVMDGVAETKAL
                     LDRFNAVSEGFADPDADMDALMAEQAELQEQIDACNGWEIERTLEIAMDALRCPPGDA
                     DVKTLSGGERRRVALCRLLLSQPDLLLLDEPTNHLDAESVAWLERFLHEYPGTVVAVT
                     HDRYFLDNVAGWILELDRGKGIPYEGNYSGWLEQKRKRLAQEEREEQARERTLAREAE
                     WIASSPRARQAKQKARITAYEDLLRAANDTAPGSSQIMIPPGPRLGATVIQFDQVSKG
                     FGDRLLIDDLSFELPPGGIVGVIGANGAGKTTLFRMIIGDEKPDNGTIKIGDTVQLGY
                     VDQSRDSLDGKKSVFDEISGGTDHLEFGKQVMNSRAYVASFNFKGGDQQKKVGVLSGG
                     ERNRVHLAKLLRSGCNVLMLDEPTNDLDVETLRALEDALLDFAGCAIVISHDRWFLDR
                     IATHILAFEGDSHVEWFEGNFEDYENDKKKRLGAEAVEPHRLKYKPLKVA"
     gene            5884..6255
                     /locus_tag="P5558_15150"
     CDS             5884..6255
                     /locus_tag="P5558_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY25703.1"
                     /translation="MARRVLIVDDEPNIVMSLEFLMKKAGFEVTVARNGREALDALAG
                     PPPDLLLLDVMMPEFDGYEVCERIRADPAWASTTIFMLTARGRDSERDRGLALGADAY
                     VTKPFSTRELVAQIEAVMASK"
     gene            6257..6448
                     /locus_tag="P5558_15155"
     CDS             6257..6448
                     /locus_tag="P5558_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25704.1"
                     /translation="MIDGRRRRYLLATLILALLLFNAPLAIVADGLGEGGYLPLYLFL
                     GWTLVILLACLVMQWRAKS"
     gene            6448..9171
                     /locus_tag="P5558_15160"
     CDS             6448..9171
                     /locus_tag="P5558_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY25705.1"
                     /translation="MFSPWFIVAAAAAYLGLLFVIASYGDRRAHEGRQLIDNPLVYTL
                     SMAVYCTSWTFYGSVGLASEAGIAFLPTYLGPTLAALLFPFVLHKILRIAKTYSITSV
                     ADFLAARYGKSTALGGIATAVAMIAGIPYIALQLKALSASANVMLGLGDGEGILPFGI
                     DSGFVIAIVMAVFAILFGTRHIDATETHQGMVLAIAFESIVKLTTFIAVGIFVTFFLY
                     DGFDSIIQATRAAGHGDLLLMPEGWNYFDWFLMILLAGSVVFVLPRQFQVAVIENVDE
                     RHIRTATWAFPTYLLLINIFVIPIALAGRLGGIENGDLIVLFLPLEAAAPWLALVVFI
                     GGLSAATAMIIVSTVALSTMISNDVVMPVLLRVEALQLTRRRHLSRLVITIRRASILL
                     LLVLAYIFFQAVGAGYGLVGIGLIAFAAVAQLVPALVLGIFWRDANLVGALAGLTAGI
                     AVWGVTLIVPALAKTGVIDPAILVDGFLGSPLLRPEALFGLDGLQPVSHALFWSITVN
                     LLLLVGGSLLFQQDNLQRLQALLFVNIFEREGTTRIWRGDTRVAELHELLLRFIGREQ
                     AEHVLATDAYRRGHRLAMEERADAEFVQLVERHVARAIGAASARAMVASVVRGEVIGP
                     DDILEILDEASQVIEYSHQLEERSRALEQAGAELKQINERLRQLDRMKDDFLATVSHE
                     LRTPLTSIRSFSEILIDNPDLELEERKEFLGIVVRESERLTRLINNVLDLSKIESGSM
                     DWHVRDVDLVAVIDEAMAATRGLASELGATLERGAVPDQPALVRGDHDRLVQVAVNLL
                     GNAFKFVPAKGGIARIGVTRDGADWVVGVEDNGPGVSLQYRDAIFEKFHQASESLKDR
                     PKGTGLGLTICQQIVSHFGGRIWVEDAPLGGACFRFRLPALPAAQTRAAAE"
     gene            complement(9214..9873)
                     /locus_tag="P5558_15165"
     CDS             complement(9214..9873)
                     /locus_tag="P5558_15165"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25706.1"
                     /translation="MSHSTSNDVTVRSRRRRNLLATAVLGATLPWLPVPWLPAGAMAA
                     VPDAEAFISQIGDEVMEILTDASKDNAQKLADLKTMLDSYTDIDLIAKLVLGRYWRTA
                     STEERETYLRLFHELLMKTLAARLGDYNGQTFEIVGSQQVSEIDSTVASRIIRVAGKP
                     PLKVDWRVREKDGSFAIIDVVAEGVSLVVSQRNEVGSIVEREGMPGLIAAMEERSGAL
                     L"
     gene            complement(9857..10618)
                     /locus_tag="P5558_15170"
     CDS             complement(9857..10618)
                     /locus_tag="P5558_15170"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRY25707.1"
                     /translation="MARSASTAAHWRAALACVAVALLLLSAAPRAAAQGVDDPLEPLN
                     RAVFQFNLIVDGTILKPVTQLYGFAVPDPAKRGVTNFLANLRAPIVFANQLLQGEREQ
                     AGITLGRFMVNTTFGLLGLVDFANYFGIPPREEADFGQTLGVYGIGAGPYLVLPILGP
                     STVRDATGIAVDSFILDPTLYFTDTPMRLGRFAITAVDTRYRYGPLIDDLQANSLDFY
                     VAARSAFLQNREAQIRKNGAGLGDEYESIFDEPLD"
     gene            10871..11137
                     /locus_tag="P5558_15175"
     CDS             10871..11137
                     /locus_tag="P5558_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25708.1"
                     /translation="MSTVRVVSFNSLRRRLATVLGDDSPLFQRFAEALGASDESLLTA
                     LIDSLDSHPPAVKKEVEGALLAWLFDAEDATGLADLAPASEARH"
     gene            11243..>11942
                     /locus_tag="P5558_15180"
     CDS             11243..>11942
                     /locus_tag="P5558_15180"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY25709.1"
                     /translation="MTPGPISPGNAPLDELLAGLRAAAEPSRLRLLAVCSHGEWTVSE
                     LVQVMAQSQPRISRHLKLLAEAGLLERFREGSWVFYRRAQSGAPARLARSLCRLLPED
                     DPLLQLDRGRLAAIRASRRRQAERFFDARAAFWDSEGDVAIEAGAVEQELIGIFAAER
                     ADSLLDIGTGTGRILQVLAGQIGSGLGVDLSHDMLRVARANLDRREARNCQVRYGDMY
                     QLPLVDGSVAAVTLH"
BASE COUNT         1804 a         3950 c         4107 g         2081 t
ORIGIN      
        1 ggccgagcgc cttgatcgtg tcgtgccgct gctgatcggt gatgatgaag acgatattcg
       61 gcctggccat gggaagatcc gtgctggaaa gggaggtcga tcccgccgcc ggcgatagcc
      121 ggccccggac gcagggaagc tagtccaagg gccgtgaccc cgccaccgcg aagtccgacg
      181 tgccgatgct cggctgcacc gtgctctacg cggccagcaa cacgctgctg acgctatggg
      241 gcctcgtgat cgtgctgctc atcctctgag ccaaccagga gaagtccggg ggaactcggt
      301 tcccccggcc ccctcgggac ctgagcccga ctagcgcaac tggctgtcct tgctgccgcg
      361 gcggttgtag agggccgccc ccggccggtc gtccagcgcc gactggcagg gcacgcaggt
      421 ccgcgcatgg ggcagggccc ggcgccgcgc ctcgggaatc tcctcgccgc agcggtcgca
      481 atgcgtagca ccctcgccgg cctgccggcg ctcgcgcgcc agccgcacgg catcgctcac
      541 cgtgtcgtcg atctgctcct gaaccgcccc gtcccgcgtc cagccaccgg ccatcgctgc
      601 gtcacctcct cgaagatcgc ggaatgcgat gataggcaag caagggcggc atgccaaaga
      661 tcatcgccac gcacgaggtc gacgacgtcg cccactggct ggccatgcag cacgacggcg
      721 ttcgaccggc gaccctcgtg gtttacgtgg aaggctgaga gcctgctttc acggggcgcc
      781 ggcgcacgcc acggcgtccg cccttccggc cccgagacgc cgctcagaag atcagggcga
      841 acaggccgat cacggccaag aggccgatca ggaagatcac gccaatggtc ccgccgatga
      901 atttcagcac gctccggtct cctggctggt tggtctcgtg cgtctggaaa cggccgaaga
      961 cgcggggcgt tccgccggag atggagtggt tgcggtcctc ctgcccccgt ccgcttcggg
     1021 acctcggcga ccggcaagct ttcgattcgt gccctgccgg cctagaatgg tgattgtggc
     1081 ggggaccatg gagagtggca gaatggcagc gcgcttggtg gatcgtatcg tagcggatcc
     1141 ccgggtgatg atgggcaagc ctgtcgtcaa ggggacgcgc attccggtcg agctcatcct
     1201 gcggcggctg ggcgccgggg acggcgacgc gcagatcctc gccgactacc ctaccctcag
     1261 catcgaggac atccgggcag cacaggcctt cgccgccgac catatcgccg gcgaggacat
     1321 cattgccgcc gaatagatga agcttctcgc ggacgaatgc gtcgccggat cgacggtagc
     1381 ggcgatgcgc gccgccggct tcgacgtgga gtggatcgcg caaacgttgc ccggcgctgc
     1441 tgatgccgat gtgctggcac gggccttcgg ggcaggacgg ctgctgctga ccgaggacaa
     1501 ggacttcggc gagctgacga tccgcttcgg ccacgccttt cacggcgtcg tcctggtcgc
     1561 ccttgccgga ttgccggcgg ccgcgcgcgc tgcccgcacc gttgcggccc ttcaggcgtt
     1621 gggtgatcga ggagccggtc aattcgtcgt catcgagcag caccgcatcc ggcgccgggc
     1681 cctgggggca cccggcaagc cggagaggcc gcgctgaagg cgcaggccgc tcgagtttct
     1741 ccggttctcg gtgcctcctt tcgcatcgac gctgcacccg tgcagggcat cgcacccatg
     1801 gcggcacatc gctcgaaagt cggccgtgag gtcccggcgc gcttcatctg aacggggacg
     1861 tggtctcgcg tccccgacct ttccgaacgt taaaacgggt tcgtgtcccg actttcccgt
     1921 gtcccgactt tccttgcccc gactttcccg aacgccccgc tattggatga gcggacggtc
     1981 cttgccggcg tcgatgatcg cctgatagac gagctgcttg acgagccggc ggtaatgagc
     2041 ggccgttggt ccgggcatct gcgccatgag gacagcggtg agcttttcct cgggatcgac
     2101 ccagaaaaag gtgcccgcgt agccggccca catgtactca ccttgcgacc ctgggacgcc
     2161 cgcgatcccg tcctcctcgc ggaccatgaa gcccaggccg aacgtgtacc catgaacgcc
     2221 catcatcggc tcaccgggcg tcaggggcgt ttggatccgc tcgccgagat ggtcggcggt
     2281 catcagcttg acgctcgtcg ggctcagcac ctgcacgcct tccagcacac cgccctggag
     2341 cagcatctgg ttgaagcgca ggtaatcacc cgctgtcgaa acggcgccac cgccgcccga
     2401 ggcattggcg ggcggcgccg agacgtcgat cagcgcgatc ggtgcgccgg cagcgttggt
     2461 cgcgaaggat tcggcgagcc gttccatggc gttctcgggc acataaaaac cggtcgagtc
     2521 catcttcagc gggccgaaca agcgcgcctc gaggaacgcg ccgagctcca tgcccgaaat
     2581 acgctcgatg acccggccga ggacgtccgt ggcgagaccg tactgccaga cctcgcccgg
     2641 ctggttcacg agctgtgccc tgccgagacc cgcgacctgc tcgtccggcg ccacgccccg
     2701 cgcgtcgaaa ggcatgccgc cgggattgaa gacgccggct tcggcataag cctgctgcac
     2761 ctccggatta cgggtgagct ccccataggc gaggcccgcg gtgtgtcgga gaaggtcctg
     2821 cacagtcatc gggcgcttca ggggcacatt gctgtaggtc gtcttgccgt cgccgtccgt
     2881 cgccgggacg ctgacctcga ggtcggcgaa ctcgggaaga tacttcgcga cgggttcggc
     2941 cagatggagc ttgccctcct ccatcaggat catcgcggcg acggagacca agggcttcgt
     3001 catcgaatag aggcggaaga tcgaatcctc ctgcatcggc tcgcccgctt gcggatcctc
     3061 catgccgagg acggccgaat aaacgagctt gccgtcacgg gcgaccatga cgacggcgcc
     3121 cggcaaagca ccgctgtcga tctcctgctg aaacacttgg ccgatcctgt cgagccgctc
     3181 gctcgacatg ccgacactct ccgcctccgc catcggcagc ggttgagcac ttacacttgt
     3241 cgtggaaaat agcgccagtg cgcccaaggc gagcgcaacc tgcttccgca tcgtcgtctc
     3301 ctcaccattc cattataggc cggcgcgtca tgcaggccgg cgaaacacct tggacctgca
     3361 tgctttgaca atcaagcggc atcaacgctt gatcaggcat ttgcacaggc gcggttttag
     3421 ttgggcgcga atgatcaatc gcggccgcca ttggcgcccc gccaaatcga gcgcaatccc
     3481 gacgagtgct gctcgtgttg cgggccgagg tctggcgctc agttttggac tgcccccctt
     3541 gggtgagacg aggggagtgt aggtttcagg cctcgttcgc ttcttgggac agctccctcg
     3601 ctgatcatga ggcggcagtt gcgtcgttca tgaggcgtcg gccaggttca ggctgtgtgc
     3661 cacgggaacg ccggtgagcg ccgcgtggag cacatccgga tcgaggtcgg cgccgttggg
     3721 ccaggtgacg gtatccgttt cggtgtcggc gacgaccttc gcgaagaagg cggtgccggc
     3781 gagcggggtg aggatgccgg tgaagccgac gatgcctgcg atgtcgactt cgccttcgac
     3841 gccgtcctcg aagcggagga agagccggtg ctggcccaaa tgacgggctt cgacgatgtc
     3901 catcagcatg catcgactcc ggcggggcga tgctcggcag tggtacttgg cggcgggcgg
     3961 tgtcgcaggc gtcgagcagc tttgcccggt ggctagccgc ccacaccgtc cagaaaaaaa
     4021 ctggtagggc ccggggagga tcatcctccc cggcttcttc cttccgtcaa gccaccttga
     4081 gcggcttgta cttgaggcgg tggggctcga cggcttcggc gccgaggcgc tttttcttgt
     4141 cgttctcgta gtcctcgaag ttgccttcga accattcgac gtggctgtcg ccttcgaagg
     4201 cgaggatgtg ggtggcgatg cggtcgagga accagcggtc gtggctgatg acgatggcgc
     4261 agccggcgaa gtcgagcagc gcgtcctcga gggcgcgcag ggtttcgacg tcgaggtcgt
     4321 tggtgggctc gtcgagcatg aggacgttgc agccggagcg gagcagcttg gccaggtgga
     4381 cgcggttgcg ttcgccgccg gagaggacgc cgaccttctt ctgctggtcg ccgcccttga
     4441 agttgaagga ggcgacgtag gccctggagt tcatcacctg cttgccgaac tcgaggtggt
     4501 cggtgccgcc ggagatttcg tcgaagacgc tcttcttgcc gtcgaggctg tcgcgggact
     4561 ggtcgacata gccgagctgg acggtatcgc cgatcttgat ggtgccgttg tcgggcttct
     4621 cgtcgccgat gatcatgcga aagagggtgg tcttgcccgc accgttggca ccgatgacgc
     4681 cgacgatgcc gcccggcggc agctcgaagg agaggtcgtc gatgagcaga cggtcgccga
     4741 agcccttgga gacctgatcg aattgtatga cggtggcgcc gaggcggggg ccgggcggga
     4801 tcatgatctg ggagctgccg ggggcggtgt cgttggcggc gcggagcagg tcctcgtagg
     4861 cggtgatgcg ggccttctgc ttggcctgcc gggcgcgggg ggagctggcg atccattcgg
     4921 cctcgcgggc gagggtgcgc tcgcgcgcct gctcctcgcg ctcctcctgg gcgaggcgct
     4981 ttctcttctg ctcgagccag ccggagtagt tgccctcgta ggggatgccc ttgccgcgat
     5041 cgagctcgag gatccagccc gcgacattgt cgaggaagta gcggtcgtgg gtgacggcga
     5101 cgacggtgcc ggggtactcg tgcaggaagc gctcgagcca ggcgacgctt tcggcgtcga
     5161 ggtggttggt cggctcgtcg aggagcagca ggtcgggctg cgagaggagc aggcggcaga
     5221 gcgccacgcg gcgccgctcg ccgccggaga gggtcttcac gtcggcgtcg cccgggggac
     5281 agcggagcgc gtccatggcg atctcaaggg tgcgctcgat ctcccagccg ttgcaggcgt
     5341 cgatctgctc ctgcagctcg gcctgctcgg ccatcagcgc atccatgtcg gcgtcggggt
     5401 cggcgaagcc ttcggagacg gcgttgaagc ggtcgaggag cgccttggtt tcggcgacgc
     5461 cgtccatgac gttgcccatg acgtccttgg tctcgtcgag gtgcggctcc tgctcgagga
     5521 agccgatctt caccccgtcc gcggcccagg cctcgccggc gaagtcctgg tccctccctg
     5581 ccatgatctt caacaggctc gacttgcccg agccgttgac gccgagcacg ccgatcttcg
     5641 cacccggcag gaaggccagg gtgatgttct cgaagagctt cttgccaccg ggaaaaatct
     5701 tggtgagccg cttcatcacg tagatatact ggtacgaggc catggtggtc cttgatggag
     5761 ggtgtgctgc cggaggcggg gttgccggct gtctagcaac tggccggggc ttcgcaaagg
     5821 gcgtcgcagg cgctagcctt cgccggcggc cgtgcagggt tcggcgaggg gagggtttcc
     5881 aacgtggcga ggcgtgtcct gatcgtcgac gacgagccca acatcgtcat gtcgctggag
     5941 ttcctgatga agaaggcggg cttcgaggtg acggtggcgc gcaacggccg cgaggcgctg
     6001 gacgcgctgg ccgggccgcc gccggatctc ctgctgctcg acgtgatgat gcccgagttc
     6061 gacggctacg aggtgtgcga gcggatccgc gccgatcccg cctgggcctc gaccacgatc
     6121 ttcatgctga cggccagggg ccgcgacagc gagcgcgacc gcggcctggc gctcggcgcg
     6181 gacgcctatg tgaccaagcc cttctcgacc cgcgagctgg tggcgcagat cgaggcggtg
     6241 atggccagca agtgacatga tcgacggccg ccgccggcgc tatctcctgg ccacgctgat
     6301 cctggcgctg ctgctcttca acgcgcctct ggccatcgtc gccgacgggc tgggcgaggg
     6361 cggctacctg ccgctctatc tcttcctcgg ctggacgctg gtcatcctgc tcgcctgcct
     6421 cgtcatgcag tggcgggcga aatcctgatg ttctcgccct ggttcatcgt cgccgccgcc
     6481 gccgcctatc tcgggctgct cttcgtcatc gccagctacg gcgaccggcg ggcgcacgag
     6541 gggcggcagc tgatcgacaa tccgctcgtc tacacgctga gcatggcggt ctattgcacg
     6601 tcctggacct tctacggctc ggtcgggctg gcctctgagg ccggcatcgc cttcctgccg
     6661 acctatctcg ggccgacgct ggcggcgttg ctcttcccct tcgtgctgca caagatcctc
     6721 aggatcgcca agacctacag catcacctcg gtcgccgatt tcctcgctgc ccgctacggc
     6781 aagagcacag cgctcggcgg catcgccacg gcggtcgcga tgatcgccgg gatcccctat
     6841 atcgccctgc agctcaaggc gctctcggcc agcgccaacg tgatgctcgg gctcggcgac
     6901 ggcgagggca tcctgccctt cggcatcgac agcggcttcg tcatcgccat cgtcatggcg
     6961 gtcttcgcca tcctcttcgg cacccgccac atcgatgcga ccgagaccca ccagggcatg
     7021 gtgctggcca tcgccttcga gagcatcgtc aagctcacca ccttcatcgc cgtcggcatc
     7081 ttcgtcacct tcttcctcta tgacggcttc gattcgatca tccaggcgac gcgtgccgcc
     7141 gggcacggcg atctcctgct gatgccggag ggctggaact acttcgactg gttcttgatg
     7201 atcctgctcg ccggctcggt cgtcttcgtg ctgccgaggc agttccaggt ggcggtgatc
     7261 gagaacgtcg acgagcggca catccggacc gccacctggg ccttcccgac atatctcctg
     7321 ctgatcaaca tcttcgtcat cccgatcgcg ctcgccggcc ggctcggcgg catcgagaac
     7381 ggcgacctga tcgtgctctt cctgccgctc gaggccgccg caccctggct ggcgctggtc
     7441 gtcttcatcg gcgggctgag tgccgcgacc gccatgatca tcgtctcgac cgtggcgctg
     7501 agcacgatga tctcgaacga cgtggtgatg ccggtgctgc tccgcgtcga ggcgctgcag
     7561 ctcacccggc gacggcatct ctcgcggctg gtcatcacca tccgccgcgc ctcgatcctg
     7621 ctgctgctgg tgctcgccta catcttcttt caggcggtcg gtgccggcta cgggctggtc
     7681 ggcatcgggc tcatcgcctt cgcggcggtg gcgcaactgg tgccggcgct cgtgctcggc
     7741 atcttctggc gcgacgccaa cctggtcggc gcactcgccg ggctcacggc gggcatcgcc
     7801 gtctggggtg tgaccctcat cgtgccggcc ctcgcgaaga ccggggtgat cgacccggcc
     7861 atcctggtgg acggcttcct cggctcaccc ctgctgcggc ccgaggcgct cttcgggctc
     7921 gacgggctgc agccggtcag ccacgcgctc ttctggagca tcaccgtcaa cctgctgctc
     7981 ctggtcggcg gctcgctgct cttccagcag gacaacctgc agcggctgca ggcgctgctc
     8041 ttcgtcaaca tcttcgagcg cgagggcacc acccggatct ggcgcggcga cacgcgcgtg
     8101 gccgagctcc acgagctcct gctgcgcttc atcgggcgcg agcaggccga gcacgtgctg
     8161 gccaccgatg cctatcgccg cgggcacagg ctggcgatgg aggagcgcgc cgatgccgag
     8221 ttcgtgcagc tggtcgagcg gcatgtggcc cgggcgatcg gcgccgcctc ggcccgcgcc
     8281 atggtggcgt cggtggtccg gggcgaggtg atcgggccgg acgacatcct cgagatcctc
     8341 gacgaggcga gccaggtgat cgagtacagc caccagctcg aggagcgatc gcgggcgctc
     8401 gagcaggccg gggcggagct caagcagatc aacgagcggc tcaggcagct cgaccggatg
     8461 aaggacgatt tcctcgcgac ggtcagccac gagctcagaa cgcctttgac ctcgatccgc
     8521 tcgttctcgg agatcctgat cgacaacccc gatctcgagc tcgaggagcg caaggagttc
     8581 ctcggcatcg tggtgcgcga gagcgagcgg ctgacgcggt tgatcaacaa cgtgctcgat
     8641 ctctcgaaga tcgaatccgg cagcatggac tggcatgtgc gggatgtcga cctcgtcgcc
     8701 gtgatcgacg aggccatggc ggcgaccagg ggcctcgcca gcgagctcgg ggcgacgctc
     8761 gagcgcgggg ccgtgccgga ccagccggcg ctggtccgcg gcgaccacga ccgtctcgtc
     8821 caggtggcgg tcaatctcct cggcaacgcc ttcaagttcg tgccggccaa gggcgggatc
     8881 gcccggatcg gcgtcacccg cgacggcgcc gactgggtgg tcggcgtcga ggacaacggc
     8941 ccgggcgtca gcctgcagta tcgtgacgcg atcttcgaga agttccacca ggcgagcgag
     9001 agcctcaagg accggcccaa ggggacgggg ctcgggctca ccatctgcca gcagatcgtg
     9061 agccatttcg gcggccggat ctgggtcgag gacgcgcccc tgggcggcgc ctgcttccgc
     9121 ttccgcctgc cggcgctgcc ggcggcccag acccgggcgg cggccgaatg agcggcggcc
     9181 agaggctgga gcgaaggccc gctcgccgcg ggctcagagc agcgcgccgc tgcgctcctc
     9241 catcgccgcg atcaggccgg gcatgccttc gcgctcgacg atgctgccga cctcgttgcg
     9301 ctggctgacg acgaggctca ccccctcggc gaccacgtcg atgatggcga agctgccgtc
     9361 cttctcgcgc acccgccagt cgaccttgag cggcggcttg ccggcgacgc ggatgatccg
     9421 gctcgcgacg gtgctgtcga tctcgctcac ctgctggctg ccgacgatct cgaaggtctg
     9481 gccgttgtag tcaccgagac gcgcggccag cgtcttcatc aaaagctcgt ggaaaagccg
     9541 cagataggtc tcacgctcct ccgtcgatgc ggtcctccag tagcgcccga gcaccagctt
     9601 ggcgatcagg tcgatgtcgg tgtaggaatc gagcatcgtc ttcagatcgg cgagcttctg
     9661 cgcgttgtcc ttggacgcat cggtgaggat ctccatgacc tcgtcaccga tctggctgat
     9721 gaaggcctcg gcgtcgggca cggcggccat ggccccggcc ggcagccagg ggacgggcag
     9781 ccacggcagg gtggcgccga gcacggccgt ggcgagcagg ttgcgccgcc gccgggacct
     9841 cactgtcacg tcgttgctag tcgagtggct catcgaagat gctttcatac tcgtcaccca
     9901 gacctgcccc attcttccgg atttgggcct ccctgttctg caggaaggcg ctgcgcgcgg
     9961 cgacatagaa gtccaggctg ttcgcctgga ggtcgtcgat caacgggcca tagcggtagc
    10021 gcgtgtcgac ggcggtgatc gcgaagcggc cgagccgcat cggcgtgtcg gtgaaataga
    10081 gcgtcggatc cagaatgaaa gaatcgacgg cgatgccggt ggcatcgcgc accgtcgagg
    10141 ggccgaggat gggcagcacg aggtaggggc cggcgccgat gccgtagacg ccgagcgtct
    10201 ggccgaaatc ggcttcctcg cgcggcggga tgccgaaata gttggcgaag tcgacgagcc
    10261 cgagcaggcc gaaggtcgtg ttgaccatga atcgcccgag ggtgatgccg gcctgctcgc
    10321 gctcgccctg caggagctgg ttggcgaaga cgatgggggc ccgcaggttc gcgaggaaat
    10381 tggtcacgcc gcgcttggcc gggtccggca ccgcgaagcc gtagagctgg gtgaccggct
    10441 tcaggatggt gccgtcgacg atcaggttga actggaagac cgcccggttc aaaggctcga
    10501 gcgggtcgtc cacgccctgc gccgcggccc gcggtgccgc cgagagcagc agcagggcca
    10561 cggccacgca ggcgagcgcc gcacgccaat gggctgccgt gcttgcggaa cgagccattg
    10621 cctatcctcg acgatgccgc agccggatcg cttggctacc gggtccggtg caggggtcta
    10681 tccgggcccc gcccggtttt caagcggcct ctagctagca cggaaaacct aagcgatgct
    10741 taacgtatgt tcatagtcac ttatgcgggc cggacgctcg ggctgtcgtg ccggcgttgc
    10801 ctggcctagg ttgcgaccgg cgatcagagg gccacgcgtg gcgctggcgg tccgacccga
    10861 aggagcatcg atgagcacgg ttcgcgtcgt ttcgttcaat tcgctcaggc gtcggctggc
    10921 cacggtgctg ggggacgatt cgcccctgtt ccagcgcttt gccgaggccc tcggcgcgtc
    10981 ggacgagagc ctgctcacgg ccctgatcga ttccctcgac agccatccgc cggcggtcaa
    11041 gaaggaggtg gagggggcgc ttctggcctg gctcttcgat gcggaggacg cgacgggcct
    11101 ggccgatctg gcgcccgcca gcgaagcccg gcactgaccc gaaggggcgt ccagcaactg
    11161 aagtcgggct tgccaaggcc gggacaacga tataaagata tcggcatatc cttctttcgc
    11221 catgcggaag ctgcggacgc cgatgacgcc aggaccgatc tcgccgggaa acgcgccgct
    11281 cgatgagctg ctcgccggcc tgagggccgc cgccgagccc tcgcggctgc ggctgctcgc
    11341 cgtctgcagc cacggcgagt ggacggtgag cgagctggtc caggtgatgg cgcagagcca
    11401 gccccggatc tcgcggcatc tcaagcttct ggccgaggcc ggcctgctcg agcggtttcg
    11461 cgagggcagc tgggtcttct atcgtcgggc gcagagcggg gcacctgccc ggctcgcccg
    11521 ctcgctctgc cgcctcctgc ccgaggacga tccgctgctg cagctcgatc gcgggcggct
    11581 ggcggcgatc cgggcctcgc gccggcggca ggccgagcgc ttcttcgacg cccgtgccgc
    11641 cttttgggac agcgagggcg acgtcgccat cgaggccggc gccgtcgagc aggagctcat
    11701 cgggatcttc gcggccgagc gcgccgactc gctgctcgat atcggcaccg gcaccggccg
    11761 gatcctgcag gtgctggccg gccagatcgg cagcggcctc ggggtcgatc tcagccacga
    11821 catgctccgg gtggcgcggg ccaacctcga ccggcgcgag gcccgcaact gccaggtccg
    11881 ctatggtgac atgtaccagc tgccgctggt cgacggcagc gtcgcggcgg tgaccctgca
    11941 cc
//
LOCUS       DAOTFI010000149        11711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6535_length_11711_cov_7.192347, whole genome shotgun sequence.
ACCESSION   DAOTFI010000149 DAOTFI010000000
VERSION     DAOTFI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6535_length_11711_cov_7.192347"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="P5558_15185"
     CDS             complement(<1..561)
                     /locus_tag="P5558_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25710.1"
                     /translation="MSPALSLPAAQFSTPSVDALALLPILIVLGAALIGVLVEAFVPR
                     GQRYSTQVVLTVVSLLAAMGSLVVTRDHQGITAGPRPGVDTLKGSVVVDGPTLFLQLV
                     ILLFAIIAVLTFAERLGGVGSDAFTPMGAATPGSPLEAQARRLGAMTSEVFPLFLFSV
                     GGMLLFPASGDLLTMFVALEVLSLPLY"
     gene            complement(558..2078)
                     /locus_tag="P5558_15190"
     CDS             complement(558..2078)
                     /locus_tag="P5558_15190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRY25711.1"
                     /translation="MTSFPWLTTIGLIPLLGALVLWLVRPLAAYAKHIALGASLVSLV
                     LTVVATLQLRSGVSGMQLVEQHAWIPQFGVSYALGVDGIAMALIVMSAILVPICLLAA
                     WNDLPADQAHREPHYFALMLVLETFMIGVFAATDVFLFYVFFEAMLIPVYFLIGQFGG
                     PRRQYAAMKFLLFSLAGGLIMLAAVIALYVQGPGGPQGFLLERLAGISLTPGTEKLLF
                     LGFFIAFAVKAPMVPVHTWLPDAAAESRPATATLLVGVLDKVGTYGMIRFCLELFPHA
                     SQWATPVVLVLAVVSVIYGALLAIGQTDIMRLIAFTSISHFGFIVLGIFALTSTGWVG
                     SSLYMVNHGFSTAALFLFAGMLISRRGSKRIPDFGGWQRVVPALAGIWLVAGLSCIAL
                     PGLGSFVSEFLVLAGTLQRHPVLAVIATTGIILAALYILLMYQRMMTGPKPEEYAATP
                     DLSTREKWVAAPLLALFLVLGFYPKPALDLITPSVQSILSSISVSDPAPAAEGSTK"
     gene            complement(2093..4057)
                     /gene="nuoL"
                     /locus_tag="P5558_15195"
     CDS             complement(2093..4057)
                     /gene="nuoL"
                     /locus_tag="P5558_15195"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRY25712.1"
                     /translation="MSLIVQVATDQLALVAGDTVPAVHGAAGITAYGWLLVALPLLGA
                     AILLLGGRRTDSWGPLLAVAMSWAAFLVGLGIFLQLLGYAPEERARHLHLFSWVPAGQ
                     FNLDAGMLFDPLSLCFVLLITFVGSLIHVYSLGYMEHDPDKRRFFAYLNLFVAAMLLL
                     VTADSYLLVYVGWEGVGLASYLLIGFWNWNPPYASAANKAFVANRVGDLGMGVAIMLM
                     FWQFGAVDFRTVDAGVAHANQGALTAIGLFLLLAACGKSAQFPLQSWLGDAMAGPTPV
                     SALIHAATMVTAGVYLVVRSHTIFDAAPNAQTAVVIVGAITLLFGAIVGCAKDDIKKA
                     LAASTMSQIGYMMLAAGLGPVGYAFAIFHLLTHGFFKAGMFLGAGSVMHGMNDSVDMR
                     RFGNLSGVMKITWVTFGLGWLAILGIPPFSGFWSKDKIIESAFVGEGWRPWVFGLATL
                     IGAGITAFYMSRLFFMTFHGEKRWPKDAHPHESPALMTYPMIVLAAGSAFLGLILGPT
                     NGLLHWLEPVVGGHEGEHHPVIPAVPLMIITLLVVAAGVYYAWMRYWRDRVPTTAPAG
                     NLVTVAARNDLYQDQVNEGLLMRPGIHLTRALVFADKRGVDGAVGGLAALIGGTSARV
                     RKLQNGLARSYALTMLLGVLLLVGAVWVMQ"
     gene            complement(4071..4370)
                     /gene="nuoK"
                     /locus_tag="P5558_15200"
     CDS             complement(4071..4370)
                     /gene="nuoK"
                     /locus_tag="P5558_15200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRY25713.1"
                     /translation="MSLMNYIYLSVLLFTIGSAAVLLRRNAIIVFMGVELMLNAANLA
                     FVTFSRMHGRLDGQVIALFVMVVAAAEVVVGLAIIVAIFRARRSASVDDANLLKL"
     gene            complement(4367..5170)
                     /locus_tag="P5558_15205"
     CDS             complement(4367..5170)
                     /locus_tag="P5558_15205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRY25714.1"
                     /translation="MTGQLTDVTGSGEAVLFWILAPLMVLGALGLIFARKAVHAALCM
                     AFVMVNLGVLYLAQQAEFLGVIQVFVYTGAVMMLFLFVLMLVGVDSSDSLVETIRGQR
                     LATAVICLGLVVLMFLAIGSMGTPTIVGLDGPNGANTDAGGNVTGLAYKIFGDYVFVF
                     EVTSALLITAALGAMVLAHRERLVAKATQRELAERRVKANSYVAGLPAPGVYARHNAV
                     DTPALLPDGTPSELSVSRVLVARDQITTPSRYADAERQAEQEIEEGRAR"
     gene            complement(5167..5925)
                     /gene="nuoI"
                     /locus_tag="P5558_15210"
     CDS             complement(5167..5925)
                     /gene="nuoI"
                     /locus_tag="P5558_15210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRY25715.1"
                     /translation="MADRPADDTGLPAERPTARPPATAGSAASASEPTPRAGWLGESL
                     PASIGGFGVSFATMFRKVLTEEYPEEKRPTAPRYHGRHQLNRHPDGLEKCVGCELCAW
                     ACPADAILVEGASNDDETGQRFSPGERYGRVYQINYLRCIFCGLCIEACPTRALTMTN
                     FYELADHTRADLIFTKEQLLAPLQEGMLPAPHPMVDGMEERDYYLGKVSAATDEQREW
                     VEAHSNATLEGVDPDVDTPADAEAEMVAEGGPAR"
     gene            complement(5918..7339)
                     /gene="nuoH"
                     /locus_tag="P5558_15215"
     CDS             complement(5918..7339)
                     /gene="nuoH"
                     /locus_tag="P5558_15215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRY25716.1"
                     /translation="MSALIALPQLPAVAGGWAAVEQPVADFSDTPLWLSFVKALLIFV
                     YLLVSTLLVIWFERRIIGRMQQRPGPNRNGPFGLLQTLADGMKSMFKEDVAPKNADKV
                     VFTLAPLIAATMCFVSFGIIPLGGTVEMFGHRTPLQLTDAPLATLLVLAVAAVGAYGI
                     LMAGWSSGSTYPLLGGLRSTAQIISYEIAMGLALVAVFLASGSMSTSSIVEAQNGRIW
                     FIIAALPSFVIYCITMVGETNRLPFDLAEGEGELTGGFHTEYGSMRFAMFFLGEYINM
                     FTVAALATTLFLGGWTLKIPGLGENGAPLALDGGWWGLLWFTLKMWSFMFVFVWLRGS
                     LPRVRYDQFMRLGWKILIPGAVVWVVLASLIRAATNGWLGTGSLSLPGGRTISTAVAV
                     VVGVVIVLAVLVAFIFDRGEPPAAAEPPSEIDPYAGGYPVPPLPGQTLKEPQRGIPAV
                     RAGAAVRSDSVGTPVTEQEDHHG"
     gene            complement(7336..9846)
                     /locus_tag="P5558_15220"
     CDS             complement(7336..9846)
                     /locus_tag="P5558_15220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HRY25717.1"
                     /translation="MTATTTSGAPAAGTPGSGPGESAPPKPELVNLTIDGIDVSVPKG
                     TLVIRAAEQVGIEIPRFCDHPLLDPVGACRQCLVDVAMPGPPGPDGKPTAARPMPKPQ
                     ASCTMTVSEGMVVNTQHTSPVADKAQQGVMELLLINHPLDCPVCDKGGECPLQNQAMS
                     NGRAVSRFEDVKRTFPKPVNISTQVLLDRERCVLCQRCTRFSDQIAGDPFINLVERGA
                     MQQIGIYEEDPFQSYFSGNTVQICPVGALTGAAYRFRSRPFDLVSTPSVCEHCASGCA
                     VRTDHRRGVVLRRMASNDPEVNLEWNCDKGRWAFQYATLPDRLTLPLVRQADGSMRVV
                     GWPEALETAAAGLRAAGSAGVLVGGRVSAEDAYAYAKFARLALGTNDVDFRARPHSAE
                     EADFLARHVVSRGPGAAVTYADLDAASAVLLVGFEPEEESPIVFLRLRHAVRKSKTVV
                     YAVAPFASRGVTKLNGRLVPTAPGAEAAALGQLRSTEAGAAAADALGAGAVILVGERL
                     ATSAGALAAAAELAAGTGARLAWVPRRAGERGALEAGALPGLLPGGRPASLAGARAEV
                     SDAWDCGELPSTAGRDTAGMIRAAAAGELGALVIGGVDPADLGLAETTAALERAFVVS
                     LEVRESAATAHADVVLPVAPQAEKSGSFVNWEGRIREFEAALDTSAMSDHRVLDMLAA
                     ELGEFLETRTQEQIHAQFQQLGAWTGNPPLVVPSGAPAPVSRRAAEPARKVVLSTWAT
                     LLDAGRLQDGEPFLAGTAKPVVARVAPALAERLGVAEGELVTVATAHGSISAPVQVAE
                     DMVDDVVWLPTNSVGCAVRATLLADSGTEVSVTKGGAQ"
     gene            complement(9843..11159)
                     /gene="nuoF"
                     /locus_tag="P5558_15225"
     CDS             complement(9843..11159)
                     /gene="nuoF"
                     /locus_tag="P5558_15225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRY25718.1"
                     /translation="MTATLTPVLTAHWDAERSWTLAAYERTGGYAALRAALAMPAADV
                     LTMVKDSAIRGRGGAGFPAGMKWSFMSKPDGGPRYLVVNADESEPGTCKDIPLMMANP
                     HALIEGAAISQYAMGSDHAFIYLRGEVVHVYRRLLRAVEEATAAGYLGDDVLGKGIRL
                     QVTAHAGAGAYICGEETALLDSLEGRRGQPRLKPPFPGAAGLYARPTSVNNVETIASV
                     PSIISNGAGWFKEMGTEKSTGYGIFSLSGHVTNPGQFEAPLGITMRELIDLAGGIRKG
                     HKLKFWTPGGSSTPIFTDEHLDVPLDFDSVAAAGSMLGTRALQVFDETVSVVRAVSRW
                     TDFYAHESCGKCTPCREGTWWLKQIMERLEHGQGTEGDIEKLVDICDNILGRSFCALG
                     DAATSPITSAVKYFREEFEAGMHTPWWETFPPERSVLFQTKEAASA"
     gene            complement(11156..>11711)
                     /locus_tag="P5558_15230"
     CDS             complement(11156..>11711)
                     /locus_tag="P5558_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY25719.1"
                     /translation="GVCTNTLCAIMGGDAIWDAVSEHLGVGHDETTADGKITLERVEC
                     NAACDYAPVVMTNWEFFDNQTPESTIALVDDLRAGRDVAPTRGAAKVCTFKEVSRVLA
                     GFHDGRADEGVGAGPASLLGLRIAKEHGWTAPEGDAARPAQQPEPAPAAAPAAATAPV
                     APETGDEHSSADRPVKGAATEEAK"
BASE COUNT         1890 a         3850 c         4450 g         1521 t
ORIGIN      
        1 gtagagcggc agcgagagca cctcgagcgc gacgaacatc gtcagcaggt cgccgctggc
       61 cgggaagagg agcatgccgc cgaccgagaa gaggaacagc gggaagacct cggaggtcat
      121 cgccccgagc cgccgggcct gggcctcaag cggtgagccg ggggtcgcgg cgcccatggg
      181 cgtgaaagca tccgagccga ccccacccag ccgctcggcg aaggtgagca ccgcgatgat
      241 cgcaaagagc aggatcacca gctggaggaa cagggtcggt ccgtcgacga ccaccgaccc
      301 cttgagggtg tccacacccg gccgcggccc ggcggtgatg ccctggtggt cgcgggtgac
      361 gacgagcgag cccatcgcag cgagcaggct gacgacggtg agcacgacct gggtggagta
      421 gcgctggccg cgcggcacga aggcctcgac gagcaccccg atgagcgccg ccccgaggac
      481 gatgaggatc ggcagcagcg ccagcgcatc gaccgaggga gtgctgaact gggcagcggg
      541 aaggctgagc gcggggctca cttggtgctc ccttcggccg cgggggcggg atcggagacc
      601 gagatcgacg acaggatgct ctgcacgctc ggcgtgatga ggtcgagcgc tggcttcggg
      661 tagaagccga gcacgaggaa cagggccagc agcggcgcgg cgacccactt ctcccgcgtg
      721 ctcaggtccg gcgtcgcggc atactcctcg ggcttgggcc cggtcatcat ccgctggtac
      781 atgagcagga tgtagagcgc ggcgaggatg atgccggtcg tcgcgatgac ggcgagcacc
      841 gggtgccgct gcagggtccc ggcgagcacg aggaactcgg acacgaagga gccgagccct
      901 ggcagcgcga tgcaggacag gccggcgacc agccagatgc cggccagcgc cggcacgacc
      961 cgctgccagc cgccgaagtc cgggatccgc ttgctgccgc gccggctgat gagcatgccg
     1021 gcgaagagga acagggccgc ggtggagaag ccgtggttga ccatgtagag gctcgagccg
     1081 acccagccgg tgctcgtcag tgcgaagatg cccagcacga tgaagccgaa gtggctgatc
     1141 gaggtgaagg cgatgagccg catgatgtcg gtctggccga tcgcgagcag ggcgccgtag
     1201 atgaccgaga cgaccgcgag cacgagcacc acgggagtgg cccactggct cgcatggggg
     1261 aacagctcga ggcagaaccg gatcatcccg taggtgccga ccttgtcgag gacgccgacg
     1321 agcagggtgg ccgtggcggg gcgagactcc gccgcggcgt ccggcagcca ggtgtgcacc
     1381 ggcaccatcg gcgccttcac cgcgaaggcg atgaagaagc cgaggaaaag cagcttctcg
     1441 gtgcccggcg tcaggctgat cccggcgagc cgctcgagca ggaagccctg cgggccgccc
     1501 gggccctgca cgtagagcgc gatgacggcg gccagcatga tcagcccgcc ggcgagcgag
     1561 aagaggagga acttcatcgc ggcatactgc cgacgcggcc caccgaactg gccgatgagg
     1621 aagtagaccg ggatgagcat cgcctcgaag aagacgtaga agaggaacac gtcggtcgcc
     1681 gcgaagacgc cgatcatgaa ggtctcgagg acgagcatga gcgcgaagta gtgcggctca
     1741 cggtgggcct ggtcggccgg caggtcgttc catgcggcga gcaggcagat cggcacgagg
     1801 atcgccgaca tgacgatgag cgccatcgcg atgccgtcga cgccgagcgc gtaggacacc
     1861 ccgaactgcg ggatccacgc gtgctgctcg accagctgca tgcccgagac gccggagcgc
     1921 agctgcaggg tcgccaccac ggtgagcacg aggctcacca gcgaggcgcc gagcgcgatg
     1981 tgcttcgcgt atgccgcgag cgggcgcacc agccacagca ccagcgcccc gagcaggggg
     2041 atgaggccga tcgtggtcag ccagggaaag gacgtcatcg ttgccacctg gttcactgca
     2101 tcacccacac ggcgcccacg aggagcagca cgccgagcaa catggtcagg gcgtaggaac
     2161 gggccaggcc gttctggagc ttgcggaccc gggccgaggt gccgccgatg agcgcggcga
     2221 gcccaccgac cgcaccgtcg accccgcgct tgtcggcgaa gacgagcgcg cgggtgaggt
     2281 ggatgccggg gcgcatcagc aggccctcgt tgacctggtc ctggtagagg tcgtttcggg
     2341 ccgccacggt gaccaggttg ccggccggtg ccgtcgtcgg cacccggtcg cgccagtagc
     2401 gcatccaggc gtagtagacg ccggcggcga cgacgagcag ggtgatgatc atcaggggca
     2461 ccgccgggat gaccgggtgg tgctcgccct cgtggccgcc gacgaccggc tcgagccagt
     2521 gcagcaggcc gttggtgggg ccgaggatga gcccgaggaa ggccgagccg gccgcgagca
     2581 cgatcatcgg gtaggtcatc agcgcgggcg actcgtgcgg gtgcgcgtcc ttgggccagc
     2641 gcttctcgcc gtggaaggtc atgaagaaga gccggctcat gtagaacgcg gtgatcccgg
     2701 cgccgatgag ggtggccagg ccgaagaccc agggccgcca gccctcgccg acgaaggcgg
     2761 actcgatgat cttgtccttg ctccagaagc cggagaaggg cgggatgccg aggatcgcca
     2821 gccagccgag gccgaacgtg acccaggtga tcttcatgac cccggacagg ttgccgaagc
     2881 ggcgcatgtc gaccgagtcg ttcatgccgt gcatgaccga gccggcaccg aggaacatcc
     2941 cggccttgaa gaagccgtgg gtgagcaggt ggaagatcgc gaaggcgtag ccgaccgggc
     3001 cgagcccggc cgcgagcatc atgtagccga tctggctcat cgtcgaggcg gccagcgcct
     3061 tcttgatgtc atccttggcg cagccgacga tcgccccgaa gagcagggtg atcgcgccga
     3121 cgatgacgac cgcggtctgc gcgttcggcg ccgcgtcgaa gatcgtgtgg ctgcgcacga
     3181 cgaggtagac accggcggtc accatggtcg ccgcgtggat cagcgccgag accggggtgg
     3241 ggccggccat cgcgtccccg agccaggact gcagcgggaa ctgcgcggac ttgccgcagg
     3301 cggcgagcag caggaagagc ccgatcgcgg tgagcgcgcc ctggttggcg tgggcgacgc
     3361 cggcgtcgac ggtgcggaag tcgacagccc cgaactgcca gaacatcagc atgatcgcca
     3421 cgcccatgcc gaggtcgccg acccggttgg ccacgaacgc cttgttcgcg gcgctcgcgt
     3481 agggcgggtt ccagttccag aagccgatga gcaggtacga cgccaggccg acgccctccc
     3541 agccgacgta gaccagcagg taggagtcgg cggtgacgag cagcagcatc gctgcgacga
     3601 agaggttgag gtaggcgaag aaccggcgct tgtccgggtc gtgctccatg tagcccagcg
     3661 agtagacgtg gatgagcgag ccgacgaagg tgatgagcag cacgaagcac agcgagagcg
     3721 ggtcgaagag catccccgcg tcgaggttga actggcccgc gggcacccag gagaacaggt
     3781 gcaggtggcg ggcccgctcc tcgggcgcgt agccgagcag ctgcaggaag atgccgaggc
     3841 cgacgaggaa ggcggcccac gacatggcga ccgcgagcag cggcccccac gagtcggtgc
     3901 gccgaccgcc gagcagcagg atggccgcgc cgagcagcgg cagggcgacg agcagccagc
     3961 cgtatgccgt gatgccggcc gcgccgtgca cggccggcac ggtgtcgccc gcgacgaggg
     4021 cgagctggtc cgtcgcgacc tggacgatga ggctcactgg tgtcgtctcc tcagagcttc
     4081 agcaggttgg cgtcgtcgac cgaggccgag cggcgggcac ggaagatggc gacgatgatc
     4141 gccaggccga cgacgacctc agccgccgcg acgaccatga cgaacagggc gatgacctgc
     4201 ccgtcgagcc ggccgtgcat gcgggagaag gtgacgaagg cgaggttggc ggcgttgagc
     4261 atgagctcga cgcccatgaa cacgatgatc gcgttgcgcc gcagcaggac ggccgcgctg
     4321 ccgatggtga acagcagcac cgacaggtag atgtagttca tcaggctcat cgggcccggc
     4381 cctcctcgat ctcctgctcg gcctggcgct cggcatcggc ataccggctg ggagtcgtga
     4441 tctggtcacg ggcgacgagc acccgggaga cggacagctc gctcggcgtg ccgtcgggca
     4501 gcagggccgg cgtgtcgacg gcgttgtgcc gcgcgtagac gccgggcgcc ggcagaccgg
     4561 cgacatagct gttggcctta acccgacgct cggcgagctc gcgctgggtc gccttggcga
     4621 ccagccgctc acggtgcgcc agcaccatcg cgccgagcgc cgcggtgatg agcagcgccg
     4681 aggtgacctc gaagacgaag acgtagtcac cgaagatctt gtaggccaag ccggtgacgt
     4741 ttccgcccgc gtcggtgttg gccccgttcg gtccgtcgag tccgacgatc gtcggggtgc
     4801 ccatcgagcc gatcgcgagg aacatcagca cgaccagccc gaggcagatc accgcggtgg
     4861 ccagccgctg gccgcggatc gtctcgacga gcgagtcgct ggagtcgacg ccgacgagca
     4921 tgaggacgaa gaggaagagc atcatcacgg cgccggtgta gacgaacacc tggatgacgc
     4981 cgaggaactc ggcctgctgg gcgaggtaga gcacgccaag gttgaccatg acgaaggcca
     5041 tgcacagggc ggcgtgcacg gccttacggg cgaagatcag cccgagcgcg ccgagcacca
     5101 tgagcggggc gaggatccag aagaggacgg cctcgccaga gcccgtcacg tcagtcagct
     5161 gaccggtcat cgggccggac ctccctcggc gaccatctcg gcctcggcgt cggcgggcgt
     5221 gtcgacgtcg gggtccacgc cctcgagcgt ggcgttgctg tgtgcctcga cccactcgcg
     5281 ctgctcgtcg gtggcggcgc tcaccttgcc gaggtagtag tcgcgctcct ccatcccgtc
     5341 gaccatcggg tgcggggcgg gcagcatgcc ctcctgcagc ggcgcgagca gctgctcctt
     5401 ggtgaagatg aggtcggcgc gggtgtggtc ggcgagctcg tagaagttcg tcatggtcag
     5461 cgcccgggtc gggcaggcct cgatgcacag tccgcagaag atgcagcgca ggtagttgat
     5521 ctggtagacg cggccgtatc gctcgcccgg gctgaaccgc tggcccgtct cgtcgtcgtt
     5581 gctcgcgccc tcgacgagga tggcgtcggc cgggcaggcc caggcgcaca gctcgcagcc
     5641 gacgcacttc tccagcccgt cggggtgccg gttgagctgg tggcgaccgt gatagcgcgg
     5701 cgcggtcggc cgcttctcct cggggtactc ctcagtgagc accttgcgga acatcgtggc
     5761 gaaggacacg ccgaagccgc cgatcgaggc cggcaggctc tccccgagcc agccggcgcg
     5821 gggggtcggc tcgctcgcgc tcgcagccga gcccgcagtc gccggcgggc gagccgtcgg
     5881 ccgctcggcg ggcaggccgg tgtcgtcggc cgggcggtca gccatggtgg tcctcctgct
     5941 cggtcacggg cgtgccgacg ctgtcggagc gcacggcggc accagcccgc acggccggta
     6001 tgccgcgctg cggctccttg agcgtctggc cgggcagcgg cgggacgggg tagccgccgg
     6061 cataggggtc gatctcactt ggcggctcgg ccgctgcggg cggctcgccc cggtcgaaga
     6121 tgaaggcgac gaggacggcg agcacgatga cgacgccgac gacgaccgcg accgccgtgg
     6181 agatcgtgcg accgccgggc agcgagaggg atccggtgcc gagccagccg ttggtcgcag
     6241 cccggatgag cgaggcgagc acgacccaga cgacggcgcc cgggatgagg atcttccagc
     6301 ccagccgcat gaactggtcg tagcgcaccc gcggcagcga gccgcgcagc cacacgaaga
     6361 cgaacatgaa cgaccacatc ttgagggtga accagagcag cccccaccag ccgccgtcga
     6421 gcgcgagcgg ggcgccgttc tcgccgaggc cggggatctt cagcgtccag ccaccgagga
     6481 agagggtcgt ggccagggcc gcgacggtga acatgttgat gtactcgccg aggaagaaca
     6541 tcgcgaagcg catcgagccg tactcggtgt ggaagccacc ggtgagctcg ccctcgccct
     6601 cggccaggtc gaagggcagc cggttggtct cgcccaccat ggtgatgcag tagatgacga
     6661 aggacggcaa ggcggcgatg atgaaccaga tccggccgtt ctgcgcctcg acgatcgagg
     6721 aggtcgacat cgagccgctg gcgaggaaga cggcgaccag ggccaggccc atggcgatct
     6781 cgtaggagat gatctgcgcc gtcgagcgca gcccaccgag cagcggatag gtcgagccgg
     6841 aggaccagcc ggccatcagg atgccgtagg cgccgaccgc ggcgaccgcg agcacgagca
     6901 gggtggccag cggggcgtcg gtgagctgca gcggggtccg gtggccgaac atctccacgg
     6961 tgccgccgag cgggatgatg ccgaaggaga cgaagcacat cgtcgcggcg atgagcggag
     7021 cgagggtgaa gaccaccttg tcggcgttct tcggcgcgac gtcctccttg aacatcgact
     7081 tcatgccgtc ggcgagggtc tgcagcaggc cgaagggtcc gttgcggttg gggcccggac
     7141 gctgctgcat ccggccgatg atccgccgct cgaaccagat gacgagcagc gtgctgacga
     7201 gcaggtagac gaagatgagc agcgccttga cgaacgacag ccacagcggc gtgtcgctga
     7261 agtcggcgac cggctgctcc acggcggccc atccgccggc caccgccggc agctgcggca
     7321 gagcgatgag ggcgctcact gggcaccacc cttcgtgacg gagacctcgg tgccggagtc
     7381 ggccagcaag gtggcgcgca ccgcgcaccc caccgagttg gtcggcagcc agacgacgtc
     7441 gtcgaccatg tcctcggcga cctgcacggg cgcgctgatc gagccgtgcg cggtcgcgac
     7501 ggtcaccagc tcgccctcgg cgaccccgag ccgctcggcg agggcgggag cgacccgcgc
     7561 gacgaccggc ttggcggtgc cggcgaggaa gggctcgccg tcctgcagtc ggccggcgtc
     7621 aagcagcgtg gcccaggtgg agagcacgac cttgcgggcc ggctcggccg cgcgacggct
     7681 gaccggtgcc ggggcgcccg agggcacgac gagcggcggg ttgccggtcc aggcgccgag
     7741 ctgctggaac tgggcgtgga tctgctcctg ggtgcgcgtc tcgaggaact cgccgagctc
     7801 ggcggcgagc atgtcgagga cccggtgatc gctcatcgcc gaggtgtcga gggcggcctc
     7861 gaactcgcgg atccggccct cccagttgac gaagctgccg gacttctcgg cctgcggggc
     7921 caccggcagg acgacgtcgg cgtgcgcggt ggccgcggac tcgcggacct cgaggctcac
     7981 gacgaaggcc cgctccaggg cggcggtggt ctcggccagc ccgaggtcgg cggggtcgac
     8041 cccaccgatg acgagcgcgc cgagctcgcc ggcggcggcg gcgcggatca tgcccgcagt
     8101 gtcgcggccc gcggtcgagg gcagctcgcc gcagtcccag gcgtcggaga cctcggcccg
     8161 agcaccggcc agcgaggccg gccgaccgcc gggcagcagg ccgggcagcg caccggcctc
     8221 gagcgcacca cgctcgccgg cgcgccgggg cacccaggcc agtcgggcac cggtgccggc
     8281 ggccagctcg gccgcggcgg ccagcgcccc agcactggtc gcgagccgct cgccgacaag
     8341 gatgaccgcg cccgcgccga gcgcgtcggc ggccgcggcg cccgcctcgg tgctgcgcag
     8401 ctggccgagc gcggcggcct cggccccggg tgccgtcggc accaggcggc cgttgagctt
     8461 ggtcacaccg cgcgaggcga acggcgcgac ggcatacacg acggtcttgc tcttgcgcac
     8521 ggcgtgccgc aggcgcagga agacgatcgg gctctcttcc tcgggctcga agccgacgag
     8581 caggacggcg ctcgcggcgt cgaggtcggc gtaggtgacg gccgcgccgg gaccgcgcga
     8641 gacgacgtgc cgagccagga agtcggcctc ctcggccgag tgcggccggg cccggaagtc
     8701 gacgtcgttg gtgcccaggg ccagccgggc gaacttcgcg taggcgtagg cgtcctcggc
     8761 actgacccgg ccaccgacga gcacgccggc ggagccggcc gcccgcagcc cggcggcagc
     8821 ggtctcgagc gcctcgggcc acccgacgac ccgcatcgag ccgtcggcct ggcgcaccag
     8881 cggcagggtg agccggtcgg gcagggtggc gtactggaag gcccagcgac ccttgtcgca
     8941 gttccactcg aggttgacct cggggtcgtt gctcgccatc cggcgcagca cgacgccacg
     9001 acggtggtcg gtgcgcacgg cgcagccgct cgcgcagtgc tcgcagacgc tcggggtcga
     9061 caccaggtcg aagggccggg agcggaagcg gtaggccgcg ccggtgagcg cgccgaccgg
     9121 gcagatctgc acggtgttgc cggagaagta ggactggaac gggtcctcct catagatgcc
     9181 gatctgctgc atcgccccgc gctcgacgag gttgatgaag gggtcaccgg cgatctggtc
     9241 ggagaagcgg gtgcagcgct ggcagagcac gcaccgctcg cggtcgagca gcacctgggt
     9301 ggagatgttg accggcttgg ggaaggtgcg cttgacgtcc tcgaagcgcg acaccgcccg
     9361 cccattggac atcgcctggt tctgcagcgg gcactcgccg cccttgtcgc agaccgggca
     9421 gtcgagcggg tggttgatga gcagcagctc catgacgccc tgctgggcct tgtcggccac
     9481 cggcgaggtg tgctgggtgt tgacgaccat gccctcggag acggtcatcg tgcacgaggc
     9541 ctgcggcttg ggcatcggcc gggctgccgt gggcttgccg tccggcccgg gcgggccggg
     9601 catggcgaca tcgacgaggc actgccggca ggcgccgacc gggtcgagca gcgggtggtc
     9661 gcagaaccgc gggatctcga tgccgacctg ctcggcggcc cggatgacga gcgtgccctt
     9721 gggcaccgac acgtcgatgc cgtcgatggt gaggttgacg agctcgggct tcggcggggc
     9781 gctctcgccc ggcccgctgc ccggggtgcc ggcggcgggg gcgccgctgg tggtggtcgc
     9841 cgtcatgcgg acgcggcctc cttggtctgg aagagcaccg agcgctccgg cgggaaggtc
     9901 tcccaccacg gggtgtgcat gccggcctcg aactcctcgc ggaagtactt caccgcggag
     9961 gtgatcgggc tggtggccgc gtcgccgagc gcgcagaacg agcggccgag gatgttgtcg
    10021 cagatgtcga cgagcttctc gatgtcgccc tcggtgccct gcccgtgctc gagccgctcc
    10081 atgatctgct tgagccacca ggtgccctcc cggcacgggg tgcacttgcc gcacgactcg
    10141 tgggcgtaga agtcggtcca ccggctcacc gcgcggacca ccgagaccgt ctcgtcgaag
    10201 acctgcagag cacgggtgcc gagcatcgag ccggccgcag cgaccgagtc gaagtcgagc
    10261 ggcacgtcga ggtgctcgtc ggtgaagatc ggggtcgagc tgccgccggg ggtccagaac
    10321 ttgagcttgt ggcccttgcg gatgccgccg gcgaggtcga tgagctcgcg catcgtgatg
    10381 ccgagcgggg cctcgaactg gcccgggttg gtgacgtggc cggagaggct gaagatgccg
    10441 tagccggtgg acttctcggt gcccatctcc ttgaaccagc cggcgccgtt gctgatgatg
    10501 ctcggcaccg aggcgatcgt ctcgacgttg ttgaccgagg tcggccgggc gtagaggccg
    10561 gccgcgccgg ggaacggagg cttgagccgc ggctggccgc ggcggccctc gagcgagtcg
    10621 agcagcgcgg tctcctcgcc acagatgtag gcgccggcac cggcgtgcgc ggtgacctgc
    10681 agccggatgc ccttgccgag gacgtcgtcg ccgaggtagc cggcggcggt ggcctcctcg
    10741 acggcgcgca gcagccgccg gtagacgtgc acgacctcgc cgcgcaggta gatgaaggca
    10801 tggtcggagc ccatggcgta ctggctgatc gccgcgccct cgatgagggc gtgcgggttg
    10861 gccatcatga gcgggatgtc cttgcaggtg cccggctcgg actcgtcggc gttgacgacg
    10921 aggtagcgcg gaccgccatc cggcttggac atgaaggacc acttcatgcc ggccgggaag
    10981 ccggcgccgc cgcggccgcg gatcgctgag tccttgacca tggtgagcac gtcggcggcc
    11041 ggcatcgcga gcgccgcgcg caacgcggca tacccgccgg tgcgctcgta tgccgcgagc
    11101 gtccaggagc gctcggcgtc ccagtgcgcg gtgaggaccg gggtgagggt ggcggtcact
    11161 tggcctcctc cgtggcggcg cccttgaccg ggcggtcggc cgacgagtgc tcgtccccgg
    11221 tctcgggggc gacgggggcg gtcgcggcag cgggggcggc ggctggcgcg ggctcgggct
    11281 gctgcgcggg gcgcgcggcg tcaccctcgg gggcggtcca gccgtgctcc ttggcgatcc
    11341 gcaggccgag cagggaggcc gggccggcgc ccacaccctc gtcggcgcgg ccgtcgtgga
    11401 agccggcgag cacccgcgag acctccttga aggtgcagac cttcgcggcg ccgcgggtcg
    11461 gcgcgacgtc gcgaccggcg cgcaggtcgt cgacgagggc gatcgtcgac tcgggcgtct
    11521 ggttgtcgaa gaactcccag ttggtcatga cgaccggcgc atagtcgcac gcggcattgc
    11581 actcgacccg ctccagggtg atcttgccgt cggcggtggt ctcgtcgtgc ccgaccccga
    11641 ggtgctccga gacggcatcc cagatggcgt cgccgcccat gatcgcgcac agcgtgttgg
    11701 tgcagacgcc g
//
LOCUS       DAOTFI010000150        11679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6559_length_11679_cov_7.158035, whole genome shotgun sequence.
ACCESSION   DAOTFI010000150 DAOTFI010000000
VERSION     DAOTFI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6559_length_11679_cov_7.158035"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="P5558_15235"
     CDS             <1..471
                     /locus_tag="P5558_15235"
                     /inference="COORDINATES: protein motif:HMM:NF019951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25720.1"
                     /translation="TIHRFARQLSGGQRQRLMVGRAMMLGPRLLVADEPVSMVDASLR
                     SSILGNLRRMKDEQGISIIYITHDLATAYEVSDYVLVLYRGRVVEAGDAETVIKDPLH
                     PYTRLLVGSIPWPDPDRHWGGDEQAEAELAERDRQGGREPCLVRSTIPGFSLAA"
     gene            505..1161
                     /locus_tag="P5558_15240"
     CDS             505..1161
                     /locus_tag="P5558_15240"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY25721.1"
                     /translation="MQLQSIKDRVRDDIVKGSFDPEARLTIDMLASRYGSSHMPIREA
                     LRELAGEGLVRFEPRRGARTLPIDRKYIDNLLTMRAELEPLLARQAAARMDALGLARL
                     EALQAELEAAVDRADDSAGIDANRRFHREINRIADNAEAFAVVDRHWLLLARLWAHYG
                     YASDRLPGVISDHRCLLQAFASGAADDAAAVSKAHVIKAKYQLLAQFDAFWQTRTIRR
                     "
     gene            1158..2297
                     /locus_tag="P5558_15245"
     CDS             1158..2297
                     /locus_tag="P5558_15245"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY25722.1"
                     /translation="MTTPATIAEADLQLLRITNKTVWAFLRLETKDGRVGWGEATIAN
                     GEEVLREVAGRFLPGLRGAPVDLGLVEKLRRAEPTLAEAAFASSLDMALLDLQGQALV
                     KPIHALLGGAVRQDIPLYANINRRTSPRTPEGFAASAGVATAAGYTIFKLAPFDEVSV
                     ERCARGDIAALMAPGLARVRATREAIGPEATLRVDCHWRFDHAAAEAMIDACRELALD
                     WIECPVPEVADAAPAIAALRRRAAGQGCRLAGLEMGTSPAAFEPFLRAGSYDVIMPDV
                     KYLGGLARFQELAASAARHGAIVSPHNPTGPICHAASLHVAAVLPELDSLEVQFDETP
                     LFDELVGGVVSPVVAGAAALPAGSGLGVAIDPVVAARLRLGEAAS"
     gene            2294..3181
                     /locus_tag="P5558_15250"
     CDS             2294..3181
                     /locus_tag="P5558_15250"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY25723.1"
                     /translation="MNEPMARHELGATLGESPVWCERTGRLWFVDIRAPAVLALDPAT
                     GELQRFPMPGLAGMVALAMGGLVVGVGCSIHPFDPATGRLADPLAVLDADRPGNRIND
                     TKAGPDGALWCGTMQDGGGASTGRLHHVEPTGFARELLDGIRCPNAIAFSPDGRTLYF
                     TDTRQGEILQAAVPTGDEKLGLRPFAPADIAPGAPDGSTVDAEGCLWNARYGGSAIVR
                     LDPDGRLMSTLHLPVSQPTACAFGGPDRRTLYVTSARQRLSPAALEGEPMAGDLLAFD
                     VGVAGLPEPRFGDASKADL"
     gene            3178..4068
                     /locus_tag="P5558_15255"
     CDS             3178..4068
                     /locus_tag="P5558_15255"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRY25724.1"
                     /translation="MKIGIIGLGDIGGNLAAWLASCGHEVTGYDIDPAAAARAAARGV
                     TPAGSRAELVERAEVIVTSLADLAAVRTTYFAEDGLIALAGAGRVTVECSTLPPDLAR
                     QITAARCAGGGVAIEAAVIGIGKDALAGQLYLMAAGDPASIERARPFLDAAGRGWLLT
                     GASGTAAIAKVLNNGVGAATLCAIAEALALAAHHGIAPATLVEAMRTGAGAGASVVLD
                     RHGAFMAEAEASTRPFNPIASKDAAALGALLPDAVATALPMLAGMVATYRETLTDAGA
                     AAPEVLTRCAHRRLKDATST"
     gene            4065..5534
                     /locus_tag="P5558_15260"
     CDS             4065..5534
                     /locus_tag="P5558_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY25725.1"
                     /translation="MTSPASPKPANLVIVLSDEHAAQYTGCHGHPIVRTPNIDRLAAT
                     GTRYANAYTNSPICMPARAAFATGRYPHQTGCWCNASPYAGTPPTWGHRLQAAGRPVV
                     SIGKLHYRDDEVDAGFDEQRIPMHAVAGIGDLQGAMRQTAPLAPRRKSHVVADEIGAG
                     ESSYTRYDRAIAREAVSWLENEAPGLDEPWVLQIGFVAPHFPLIAPEEFFAMYPPESL
                     PLPKACRPEDWPRHPWFDELRKAYITDTFFDDDKRRIATAAYFGLCSFVDHHLGLVRD
                     AIERAGLGQSTRLVYLSDHGDNLGVRGLWQKSNFYEESAHIPLLMSGPGIAAGEVVRT
                     PVSMIDFFPTILDAVGVASDPADRDLPGQSLFRLAQDDRERTVFGEYHAAGAVSGAYM
                     LRRDKWKYVHYAGYPAQLFDLEADPEELTDLASDAAHATTLAGFERELRAMLDPEATD
                     ARAKADQAAIIERHGGVEAVLARGSFGATPPPGEKAALG"
     gene            complement(5551..5946)
                     /locus_tag="P5558_15265"
     CDS             complement(5551..5946)
                     /locus_tag="P5558_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY25726.1"
                     /translation="MKIQRSGATPSVTPPAENFTGKVRFDAPFGGSGEGQVGGAAVTF
                     EPGARTAWHTHPFGQTLIVTAGLGWAQREGGPKEEIRPGDIVWFEPGEKHWHGGTATT
                     AMTHIAIALAKDGSRATWLEKVSDADYLG"
     gene            complement(6018..7856)
                     /locus_tag="P5558_15270"
     CDS             complement(6018..7856)
                     /locus_tag="P5558_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY25727.1"
                     /translation="MRSGAESLWSTHEIEIELLRLLKAMHRFQAPDPGVDLEALRLRL
                     DILWSRLPPLLEGRDAAQLREASDVETLGARLSAALQEISDILDAPSVDAAAYARAMA
                     GLEAFATPIRELSLVALHQTSQGHAELQRRLDDIYTAILMASCGLGLGTLGLITLLVF
                     QLGRSQKLLDLKERANAQIEHLAHHDVLTGLPNRRLFNDRLAQSLAAAARRSSGVGLH
                     FIDVDNFKAINDTFGHMAGDQVLQEAARRMAATMREVDTLARIAGDEFAAIQIDVATA
                     DGVVELSERLIRAFEQPFDVAGNDLQASISIGSTLFPDDGRTTERLLSNADVALHRAK
                     QNGRRQGALFDEVMMEKEARRRRLGVRLEQAREHGELSIRYQPKFATADGRLMGFEAL
                     IRWDRSPHVEVSPTEFIPIAESNGSIVPIGDWVLWTACRDLAGSAARHRPDVTLAINL
                     SPVQIQKEDCFRRFLKIVESLDFDPRRLEFEITETSLFNADTSTLANIRDLHDVGARI
                     CLDDFGTGYSSLSHLQALPLNSMKLDRGFVARLDDHRTQCICEGITALGHRLGLEIVG
                     EGAETEWQYNELVRMGCDQVQGYYLGRPMPIEELDGFMARLARAPA"
     gene            complement(7963..8457)
                     /locus_tag="P5558_15275"
     CDS             complement(7963..8457)
                     /locus_tag="P5558_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25728.1"
                     /translation="MLAVTAVAGMSSGTEASDLEPPGAEVILTAKGLLGRTNVGDEAR
                     LDRQLLESFGKATLTTWTPWTEGEVEFEGLPLRSLVTGLQAGGTAMLLRALNDYHVVI
                     PMSEARDYDLLIAWRADGVDLTRRDRGPLWIIYPWSSHPDLDRRVTSQRAIWQLYELE
                     FYED"
     gene            complement(8725..9090)
                     /locus_tag="P5558_15280"
     CDS             complement(8725..9090)
                     /locus_tag="P5558_15280"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY25729.1"
                     /translation="MNRQPAVLQRTDWATDERLWRGRFEGRELGTGVTVLFFASEKMG
                     AGPVLHVHPYDEVFIVRVGRALFTLGSETVTAEAGQILLCPAHVPHKFANLGPGLLET
                     TDIHLSDRLIQTDLPDPQA"
     gene            9275..9910
                     /locus_tag="P5558_15285"
     CDS             9275..9910
                     /locus_tag="P5558_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25730.1"
                     /translation="MPVRADLKRLGRVWSWPAILLLASVIAGCTVSAGRDFARPSDGS
                     LAIGSTTESQVMSQYGQPLSTGTEDKNGVTVNKLIYTYSSNEEALVGGITPVRSLAFF
                     FVDRVLVGYEFVSSYPADHTSFDETKRSMITKGVSTEADVRALLGRPNGEFGPPFVEQ
                     PTVRALVYSYQQVQVEIGFFSGEMHHGTKRLFIELDADGIVRDYKFESATT"
     gene            10041..10406
                     /locus_tag="P5558_15290"
     CDS             10041..10406
                     /locus_tag="P5558_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25731.1"
                     /translation="MVALSACSVGMAAAGKESPNLAVCQVGSDKTLIESEVGPPKTIE
                     SLADGSTSCTYEYEFGNEPSAGRAVLNAGMDVVTFGLWEVVGTPAEALQGSKYEMVVV
                     YGPDGKAKEIRTRPLGIFD"
     gene            complement(10562..11455)
                     /locus_tag="P5558_15295"
     CDS             complement(10562..11455)
                     /locus_tag="P5558_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY25732.1"
                     /translation="MRIVDAHHHLWDRDLHPYPWLAKPVRSPAIGDTTPLCRNYLLAD
                     FRADAAGLELVKSVHLQAEIAPELAVLETRWLQAIADAPGSGGFPHGIVAFADLAGPD
                     VDKVLEAHGAFANMRGIRQILNRHSDPALNAASADYLNDAAWQRGYARLAKHGYAFDL
                     QLYWPQMAAAADLAARHPDIPVILNHTGMPRERDPDGIEGWRQGLRQLARCPNVSAKI
                     SGLGMVDHHWTEDSIRPFVLDTIDIFGPDRCLFASNFPVDRLYSDYATVWRAYDGITK
                     DFSADERAALFHDNAVRVYRL"
     gene            complement(11460..>11679)
                     /locus_tag="P5558_15300"
     CDS             complement(11460..>11679)
                     /locus_tag="P5558_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25733.1"
                     /translation="SATRNTHFYEMALVGPGMANAVPPVYTCGYSDQPKDLGKDGCVP
                     VPDGPGLGVTYDWAYIEQHRTAHHVFGG"
BASE COUNT         1808 a         4110 c         3944 g         1817 t
ORIGIN      
        1 acgatccacc gcttcgcccg ccagctcagc ggcggccagc gccagcggct gatggtcggc
       61 cgggcgatga tgctgggccc gaggctgctg gtcgccgacg agccggtctc gatggtcgac
      121 gcctcgctgc ggtccagcat cctcggcaac ctccggcgga tgaaggacga gcagggcatc
      181 tccatcatct acatcaccca cgacctcgcc accgcctacg aggtctcgga ctacgtgctc
      241 gtgctctatc gcggccgtgt ggtcgaggcg ggcgacgcgg agacggtcat caaggacccg
      301 cttcacccct acacgcgcct gctcgtcggc tcgatcccct ggcccgatcc cgaccggcac
      361 tggggcggcg acgagcaggc cgaggccgag ctcgccgagc gcgaccgcca gggtgggcgc
      421 gagccctgcc tcgtccgctc gaccatcccc ggcttcagcc tcgcggcatg aggcacccga
      481 cccgtctgcg gtgccggggc tgacatgcag ctccagtcga tcaaggaccg ggtgcgcgac
      541 gacatcgtca agggcagctt cgatcccgag gcgcggctga ccatcgacat gctggcgagc
      601 cgctacggct ccagccacat gccgatccgg gaggccctgc gcgagctggc gggcgaaggg
      661 ctcgtccgct tcgagcccag gcggggggcc cgcaccctgc cgatcgaccg caaatatatc
      721 gacaacctgt tgaccatgcg ggccgagctc gagccgctct tggcccgcca ggcggcagcg
      781 cgcatggacg cccttgggct cgcccggctc gaggccctgc aggccgagct cgaggcggcc
      841 gtggaccgcg ccgacgacag cgctggaatc gacgccaacc ggcgcttcca ccgcgagatc
      901 aaccgcatcg ccgacaatgc cgaggccttc gccgtcgtgg accggcactg gctgctcctc
      961 gctcggctct gggcccatta cggctatgcc tccgaccgtc tcccgggcgt catcagcgac
     1021 caccgctgcc tcctgcaggc cttcgccagc ggtgccgccg acgatgccgc ggcggtgagc
     1081 aaggcgcacg tgatcaaggc gaagtaccag cttctggcgc agttcgacgc tttttggcag
     1141 acgaggacga tccgcagatg acgacaccag cgaccatcgc cgaagccgat cttcagctcc
     1201 tgcgcatcac caacaagacc gtctgggcgt tcctccggct cgagacgaaa gatggccgcg
     1261 tcggctgggg cgaggcgacc atcgccaacg gcgaggaagt gctgcgcgag gtggccggcc
     1321 gcttccttcc gggcctgcgc ggcgcgcccg tcgacctcgg cctcgtcgag aagctgcggc
     1381 gcgccgagcc gacgctggcc gaggcagcct tcgcctccag cctcgacatg gcgctgctcg
     1441 atctgcaggg ccaggcgctg gtcaagccga tccacgccct cctcggcggt gcggtgcggc
     1501 aggacatccc gctctatgcc aacatcaacc ggcgcacctc cccgcgcaca cccgaaggct
     1561 tcgccgccag cgccggcgtc gcgactgccg ccggctacac gatcttcaag ctcgccccgt
     1621 tcgacgaggt cagcgtcgag cgctgcgccc gcggcgacat cgcagcgctc atggccccgg
     1681 gcctcgcccg ggtcagggcg acccgcgaag ccatcggccc ggaggctacg ctccgggtcg
     1741 actgccactg gcgcttcgac cacgccgccg ccgaagccat gatcgacgcc tgtcgcgagc
     1801 tcgccctcga ctggatcgag tgcccggtgc cggaggtggc ggatgccgcc cccgcgatcg
     1861 ccgccctacg ccgccgggcc gccggccagg gctgccggct cgccgggctg gagatgggca
     1921 cgagcccggc ggcgttcgag cccttcctcc gggcgggctc ctatgacgtg atcatgcccg
     1981 acgtgaagta tctgggcggc ctcgcccggt tccaggagct ggcggccagc gccgcgcggc
     2041 atggcgccat cgtctccccg cacaacccga ccggcccgat ctgccatgca gcgagcctgc
     2101 acgtcgctgc cgtgctgccc gagctggatt ccctggaggt gcagttcgac gagacgccgc
     2161 tcttcgacga gctggtcggc ggcgtggtct cgccggtcgt ggcgggtgcc gccgccctgc
     2221 ccgccgggtc gggtctcggc gtggccatcg atccggtcgt ggcggcacgg ctgcggctcg
     2281 gggaggccgc gtcgtgaacg agcccatggc acggcacgag ctgggtgcca cgctcggcga
     2341 atcgccggtc tggtgcgagc ggaccggccg gctctggttc gtcgacatcc gcgccccggc
     2401 cgtgctcgcg ctcgatccgg cgaccggcga gctgcagcgc tttcccatgc ccggcctcgc
     2461 cggcatggtg gcgctggcca tgggcgggct cgtggtcggg gtgggctgca gcatccaccc
     2521 cttcgacccg gccactggcc ggctcgccga ccccctcgcg gtcctcgatg ccgaccggcc
     2581 cggcaaccgc atcaacgaca ccaaggccgg ccccgacggt gccctctggt gcggcacgat
     2641 gcaggacggc ggcggggcgt cgaccggccg gctgcatcac gtcgagccga ccggcttcgc
     2701 cagggagctg ctcgacggca tccgctgccc gaacgccatc gccttctcgc cggacggccg
     2761 gaccctctac ttcaccgata cccgtcaggg cgagatcctc caggcggccg tgccgacggg
     2821 tgacgaaaag ctgggtctcc gccccttcgc cccggccgac atcgcaccgg gcgcgccgga
     2881 cggctcgacg gtcgacgccg agggctgcct ctggaacgcc cgctatggcg gcagcgccat
     2941 cgtccgcctc gatcccgatg gcaggctcat gtcgaccctg catctgccgg tcagccagcc
     3001 caccgcctgc gccttcggcg gccccgatcg gcgcaccctc tacgtcacct ccgcgcgcca
     3061 gcgcctgtcc ccggcagcgc tggagggcga gccgatggcc ggcgatctcc tcgccttcga
     3121 cgtcggcgtc gcgggcctgc cggagccgcg cttcggcgac gcctcgaagg ccgacttgtg
     3181 aagatcggca tcatcgggct cggcgatatc ggcggcaacc tcgccgcctg gctcgcctcc
     3241 tgcggccacg aggtcaccgg ctacgacatc gacccggcgg ctgcggcgcg cgccgcggcg
     3301 cggggtgtca cgcccgcggg ctccagggcg gagctggtcg agcgggcgga ggtgatcgtg
     3361 accagcctcg ccgacctggc ggccgtgcgc acgacatatt tcgccgagga cggcctcatc
     3421 gcgctggccg gcgccggccg ggtgacggtc gaatgctcga ccctgccgcc cgacctggcg
     3481 cgccagatca cggcagcgcg gtgtgccggc ggcggtgtgg cgatcgaggc cgcggtcatc
     3541 ggcatcggca aggatgccct ggccgggcag ctctacctca tggccgcggg cgatcccgcc
     3601 tcgatcgagc gcgcacggcc gttcctcgac gccgcggggc gcggctggct cctgacaggg
     3661 gcgagcggca cggccgcgat cgccaaggtg ctgaacaacg gcgtcggtgc cgccaccctc
     3721 tgcgccatcg ccgaggccct ggccctggcg gcgcatcacg gcatcgcccc ggccacgctg
     3781 gtcgaggcca tgcgcaccgg ggcgggtgcc ggcgcctcgg tggtgctcga ccggcacggc
     3841 gccttcatgg ccgaggccga ggcgagcacc cgccccttca acccgatcgc cagcaaggac
     3901 gcggcggccc tcggcgccct cctccccgac gcggtggcca cggcactgcc gatgctggcg
     3961 ggcatggtgg cgacctaccg cgaaaccctg accgacgccg gcgcggccgc acccgaggtg
     4021 ctgacccggt gcgctcatcg acgcctgaag gatgcgacct cgacatgacg agcccagcca
     4081 gcccgaagcc cgccaacctc gtgatcgtgc tctcggacga gcatgccgcg caatataccg
     4141 gctgccacgg ccaccccatc gtcaggaccc cgaacatcga ccggctcgcc gccacgggca
     4201 cacgctacgc caacgcctac accaacagcc cgatctgcat gccggcccgc gccgccttcg
     4261 ccaccggccg ctacccgcac cagacgggct gctggtgcaa tgccagcccc tatgccggca
     4321 cgccgcccac ctggggccac cgattgcagg cggcgggccg gcccgtggtc tcgatcggca
     4381 agctgcacta ccgggacgac gaggtcgatg ccggcttcga cgagcagcgc atccccatgc
     4441 acgcggtagc cggcatcggc gacctgcagg gcgccatgcg ccagacggcc cctctggcgc
     4501 cccggcgcaa gagccatgtc gtggccgacg agatcggcgc cggcgaatcc agctacaccc
     4561 gctacgaccg ggccatcgcc agggaggcgg tgagctggct ggagaacgag gcgccggggc
     4621 tcgacgagcc ctgggtgctg cagatcggct tcgtcgcccc gcacttcccg ctgatcgcac
     4681 ccgaggaatt cttcgccatg tacccgccgg agagcctgcc cttgcccaag gcctgccggc
     4741 ccgaggactg gccccgccat ccctggttcg acgagctccg caaggcctac atcaccgaca
     4801 ccttcttcga cgacgacaag cgccggatcg ccaccgccgc ctatttcggc ctctgcagct
     4861 tcgtcgatca ccacctgggc ctggtcaggg acgccatcga gcgcgccggc ctcggccaga
     4921 gcaccaggct cgtctacctc tccgaccacg gcgacaatct gggggtccgc ggactctggc
     4981 agaaatcgaa cttctacgag gagtcggcgc atatcccgct tttgatgagc ggccccggca
     5041 tcgccgccgg cgaggtggtg cggacaccgg tctccatgat cgacttcttc ccgaccatcc
     5101 tcgacgccgt cggcgtcgcc tccgacccgg ccgaccgcga cctgccgggc cagagcctct
     5161 tccggctggc acaagacgac cgcgagcgca ccgtcttcgg cgagtatcac gcggccggtg
     5221 ccgtgagcgg cgcctacatg ctccgccggg acaaatggaa atacgtgcac tatgccggct
     5281 acccggcgca gctcttcgac ctcgaggccg acccggaaga gctcaccgac ctcgcctccg
     5341 acgccgccca tgccacgacg ctcgccgggt tcgagcggga gctgcgcgcc atgctcgatc
     5401 ccgaggccac ggacgccagg gccaaggccg accaggcggc catcatcgag cgccacgggg
     5461 gtgtcgaggc ggtgctcgcc cgcggctcct tcggcgccac cccgccgccc ggggagaagg
     5521 ccgcgctcgg ctagccggcc gccggggaag tcagccgaga tagtccgcgt cggagacctt
     5581 ctcgagccag gtggcgcgcg acccgtcctt ggccagggcg atggcgatgt gcgtcatggc
     5641 ggtggtcgcc gtgccgccgt gccagtgctt ctcgcccggc tcgaaccaga cgatgtcgcc
     5701 cggccggatc tcctccttcg gcccgccctc gcgttgcgcc cagccgaggc cggcggtgac
     5761 gatcagggtc tggccgaagg gatgggtgtg ccaggcggtg cgcgcaccgg gctcgaaggt
     5821 gaccgccgca ccgccgacct ggccctcgcc gctgccgccg aacggcgcgt cgaagcggac
     5881 cttgccggtg aagttctcgg cgggcggcgt cacggatggc gtggccccgc ttctctggat
     5941 tttcatgact tttcctttcg ctcctgtgta cccgcgtcgc tcgacccacc gaacgaccga
     6001 ccgaccgggc gcagcgttca cgccggggcc cgcgcaagcc tggccatgaa gccgtccagc
     6061 tcctcgatgg gcatcggccg gccgagatag tagccctgaa cctggtcgca gcccatgcgc
     6121 accagctcgt tgtactgcca ttccgtctcg gcaccctcgc ccacgatctc caggccgagg
     6181 cggtgaccca gcgcggtgat gccttcgcag atgcattgcg tccggtgatc gtccaggcgc
     6241 gccacgaagc cgcgatccag cttcatggaa ttcaggggaa gcgcctgcag gtgcgagagg
     6301 ctggagtagc ccgtgccgaa gtcgtcgagg cagatccggg cacccacgtc gtgcaggtcc
     6361 ctgatgttcg cgagggtgct cgtgtcggcg ttgaagaggc tcgtttccgt gatctcgaac
     6421 tccagccggc gcgggtcgaa atccagggat tcgacgatct tgaggaaacg gcgaaagcag
     6481 tcttccttct ggatctgcac cggcgagagg ttgatcgcga gcgtcacgtc gggccgatgg
     6541 cgcgccgccg agcccgccag gtcacggcag gccgtccaca gcacccagtc gccgatcggc
     6601 acgatcgagc cgttgctctc ggcgatgggg atgaactcgg tcgggctgac ctcgacatgg
     6661 ggagatcgat cccagcggat cagcgcctcg aaccccatga ggcgcccgtc ggcggtggcg
     6721 aacttgggct gatagcggat cgagagctcg ccatgctccc gggcctgctc cagacggacg
     6781 cccaggcggc gtcgtctcgc ctccttctcc atcatcactt cgtcgaagag cgcaccttgc
     6841 cgccggccgt tctgcttcgc ccgatgcagg gcgacgtccg cattggagag gaggcgctcg
     6901 gtcgtgcggc catcatcggg aaagagcgtg gagccgatgc tgatgctcgc ctgcagatcg
     6961 ttgccggcga cgtcgaacgg ctgctcgaaa gccctgatca gccgttcgct cagctccacg
     7021 acgccgtccg ctgtcgcgac gtcgatctgg atggccgcga actcgtcacc ggcgatgcgg
     7081 gccagcgtgt cgacctcgcg catcgtcgcc gccatgcgcc tggccgcctc ctgcagcacc
     7141 tggtcaccgg ccatgtgccc gaaggtgtcg ttgatcgcct tgaaattgtc gacgtcgatg
     7201 aagtgcaggc cgaccccgct cgagcggcga gccgcggcag ccagggactg cgccaggcga
     7261 tcgttgaaca gccgacggtt gggcaggccg gtcagcacgt cgtggtgcgc cagatgctcg
     7321 atctgcgcgt tggcccgctc cttcaggtcg agcagcttct gtgatcggcc cagctggaag
     7381 accaggagcg tgatcaggcc gagcgtgccg agcccgagcc cgcaggacgc catcaggatg
     7441 gccgtgtaga tgtcgtccag acgccgctgc agctcggcat ggccctggct cgtttgatgc
     7501 agggcgacca ggctcagctc gcggatcggc gtggcgaacg cctcgagccc cgccatggcc
     7561 cgggcgtagg cagcggcatc gacgctgggc gcgtccagga tgtcggaaat ctcctgcaac
     7621 gcggcgctca atcgggcgcc gagcgtctcc acatcgctgg cctccctgag ttgcgccgca
     7681 tcccgcccct cgagcagcgg aggcagccgg ctccagagaa tgtcgagcct gaggcgcagc
     7741 gcttccagat ccacgcccgg atccggtgcc tggaaacgat gcatggcctt caacaggcga
     7801 agcagctcga tctcgatctc gtgcgtcgac cacagggatt ccgcgcccga tcgcaagatc
     7861 gccgcgttgc ccagccgcag ccgttcgaaa ttgataaaaa tgatggatgt gaacgtgaca
     7921 aatacggaaa aaacaagaaa acctatcgcc aggtcacgcc aactagtcct cataaaactc
     7981 tagttcatat agttgccata tcgcgcgttg gctggtgacg cgccggtcga ggtcggggtg
     8041 agacgaccag gggtagatga tccacaaggg cccccggtcg cgccgcgtca ggtcgacgcc
     8101 gtcggcgcgc caggcgatca gcaggtcgta gtcgcgcgcc tccgacatcg ggatcaccac
     8161 atggtaatca ttgagcgctc gcagcagcat ggcggtgccg ccggcctgca ggcccgtgac
     8221 cagcgagcgt agcggcagcc cctcgaactc gacctcgcct tccgtccacg gcgtccaggt
     8281 cgtcagcgtc gccttgccga agctctcgag gagctgccgg tccagacggg cttcgtcgcc
     8341 gacattggtc cgccccagca gacccttcgc cgtcaggatg acctcggcgc ctggcggttc
     8401 cagatcgctc gcctcggtgc cgctcgacat tcccgccacg gccgtcaccg cgagcacgag
     8461 agccgcgcgc cggctccagc aaagtgacgg ccggtcggac aagcaactca aaggcgcgtc
     8521 tggatgtgca gcgtgctcca ccacgatcta caccctttga ttgtgcgcag tgcctaattt
     8581 atcgccccta gcgtgtcaac gtcggcgtga aagcgggccg ggtccggccc cctttgccct
     8641 ggcacacctg ccgccgtctt cattcgtgca agtgtgccct cgatccgcga cggacaccaa
     8701 aggacctccc gcccgcggcg cacgtcacgc ttgcgggtcg ggcaggtcgg tctggatcag
     8761 gcgatcgctg aggtggatgt cggtcgtctc gagcagaccg gggccgagat tggcgaactt
     8821 gtgcggcaca tgcgcggggc agagcaggat ctggcccgcc tcggcggtga ccgtctcgct
     8881 gcccagcgtg aagagggctc ggccaacccg gacgatgaag acctcgtcgt aaggatgcac
     8941 atgcagcacc ggccccgccc ccatcttctc gctggcgaag aagaggacgg tgacgcctgt
     9001 gccgagctcc ctgccctcga atctgccgcg ccagagccgc tcgtcggtcg cccagtcagt
     9061 gcgctgcagg acggcgggct ggcgattcat ggcttgtctc cgggtcggtt ggtgtatcgg
     9121 gcgatcgggg cctgtgcgct gactctggga cccggccatc gtgctgaaac gcagcgccat
     9181 ccgcccgatg gtcgggttgc agccgcttga tgcagatcaa ggtgattgaa ttcaagaaaa
     9241 ttataatgaa cgcaaacgcc aggggaatca gcaaatgcct gttcgtgcgg acttgaagag
     9301 gcttggtcgt gtgtggagtt ggccggccat tttattgctg gccagcgtga tcgctggctg
     9361 caccgtaagt gccggtcggg atttcgcccg cccgagcgac ggctcactgg cgattggatc
     9421 gaccaccgag tcgcaagtta tgagccaata tggccagccg ctctcgaccg gcacggagga
     9481 caagaatgga gtaacagtca ataagctgat ctacacatat agcagcaatg aggaagcgtt
     9541 ggtcggcggt attacgccgg ttcgctcgct cgctttcttc tttgtggacc gggtactcgt
     9601 cggctacgaa ttcgtcagtt catatcccgc agatcacacg agcttcgacg agaccaagcg
     9661 gtcgatgatc accaagggcg tgagcaccga ggcggatgtc cgggcactac tcggtcgccc
     9721 caacggtgag ttcggccctc ccttcgtcga gcaaccgacc gtcagggcgc tggtctattc
     9781 gtaccagcaa gttcaggttg agatcggttt cttctctggc gagatgcacc atgggacgaa
     9841 gcgtctcttc atcgagctcg acgccgatgg catcgtgcgg gactacaagt tcgagtcggc
     9901 cacgacctga gccggtccct tcggcaggat tgcatggctc gccctgccca gcgaccgcga
     9961 aacggctcgt gagaggaaga ggagcggcaa tgaaccgatg gctcgggcct ctcgcattgg
    10021 cggggtgcct cgcgggcagc atggtcgcgc tctccgcctg ctcggtcggc atggccgctg
    10081 ccggcaagga gtcgcccaat ctcgcggtct gccaggtcgg ctccgacaag accctgatcg
    10141 agagcgaggt cggtccaccc aagaccatcg aatcgcttgc cgacgggagc acttcctgca
    10201 cctacgagta cgagttcggc aacgagcctt cggcgggccg cgccgtgctc aatgccggca
    10261 tggatgtggt gaccttcggc ctctgggaag tcgtgggcac tccggccgag gccctccaag
    10321 gcagcaagta cgagatggtc gtcgtctacg ggccggacgg gaaggccaag gagataagga
    10381 ccagaccact cggcatcttc gattgacatt ccacttgtcg caagccgagg ccccgcggat
    10441 cgatccgacc gatctcgatt ggaggacact ttcgtgcgcc tgtctacaga ccgggcagga
    10501 cttggcgctg agttgggcgg gcccaccata cgtggtcaat gatctgggag cctgcccctg
    10561 cctaaagccg ataaacccgc accgcattgt cgtggaacag cgccgcccgc tcgtcagcgc
    10621 tgaaatcctt cgtgatgccg tcataggccc gccacacggt cgcatagtcc gaatacaggc
    10681 gatccaccgg gaagttgctg gcgaagaggc agcgatcggg gccgaagatg tcgatggtgt
    10741 cgaggacgaa ggggcggatg gaatcctcgg tccagtggtg gtcgaccatg ccgaggccgg
    10801 agatcttggc gctgacattg gggcatcggg cgagttggcg caggccctgg cgccagccct
    10861 cgatgccatc cggatcgcgc tcgcggggca tgccggtgtg gttgaggatc accgggatgt
    10921 cggggtgccg ggcggcgagg tcggcggcgg cggccatttg tggccagtag agctggaggt
    10981 cgaaggcgta gccgtgcttc gccagccgcg cgtagccgcg ctgccaggct gcgtcgttca
    11041 ggtagtcggc gctggcggcg ttcagggccg gatcgctgtg gcggttcagg atctggcgga
    11101 tgccgcgcat gttggcgaaa gcgccgtgcg cctccagcac cttgtccaca tcggggccgg
    11161 cgaggtcggc gaaggcgacg atgccgtgcg ggaagccgcc cgagcccggg gcgtcggcga
    11221 tggcctgcag ccagcgcgtc tccaggacgg cgagctcggg cgcgatctcg gcctgcaggt
    11281 ggacggactt cacgagctcc agcccggcgg cgtcggccct gaagtcggcg agcaggtagt
    11341 tgcggcagag cggggtggtg tcgccgatgg cgggtgaccg gaccggcttt gccagccagg
    11401 gatagggatg caggtcgcgg tcccagaggt ggtggtgggc gtcgacgatc cgcatgtgct
    11461 cacccaccaa agacgtggtg ggccgtccgg tgctgctcga tataggccca gtcgtaggtg
    11521 acgccgaggc cggggccatc ggggaccggg acgcagccgt ccttgcccag gtccttcggc
    11581 tggtcgctgt agccgcaggt gtagaccggg ggcacggcgt tcgccatgcc gggtcccacc
    11641 agcgccatct cgtagaaatg ggtgttgcgc gtggcgctg
//
LOCUS       DAOTFI010000151        11638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6583_length_11638_cov_7.709747, whole genome shotgun sequence.
ACCESSION   DAOTFI010000151 DAOTFI010000000
VERSION     DAOTFI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6583_length_11638_cov_7.709747"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5558_15305"
     CDS             <1..230
                     /locus_tag="P5558_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY25734.1"
                     /translation="WFGIFIVVVVEMAQITPPVGFNLFVMQGITGHNILYVARTALPF
                     FLLLVVMVGLLVAFPGLATWLPEQMRQTAAG"
     gene            277..1155
                     /locus_tag="P5558_15310"
     CDS             277..1155
                     /locus_tag="P5558_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY25735.1"
                     /translation="MSAQRGPFDRARHRRHRRRFPRGPDFLRRAIEERLLDRLDDIRT
                     PLGRVLVLGAQDGGLADAIRGRPGVDAVVTADELAPADVLLDEELLPFREGAFSTVLS
                     AMSLHWVNDLPGLLAQVRHCLAADGLLLAAFPGGETLHELRQALLAAELDVTGGAGLR
                     LSPMIDVRDAGALLQRAGFALPVADVERLTVRYAEPLRLLMDLRAMGEAQALGEGARG
                     SLHRPVLGRLDAILRERFADPDGKVRASFDIVTMTGWKPHASQPRPLRRGSGEVSLAK
                     ALGVPLEVLQGKAKKA"
     gene            complement(1165..1638)
                     /locus_tag="P5558_15315"
     CDS             complement(1165..1638)
                     /locus_tag="P5558_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25736.1"
                     /translation="MARSVRRAAGMFSLGFAMVALAGCSGGPESLPIDAYLAPPPLWR
                     GLPPPAPVPDLARNVELVGMDSGDLRSVFGEPALVRVEGNVQYWRYAFAGCSLDLFVD
                     ARPDRQAEVVHYDLRPEPLYGNGKAAAGCERLARQLDQGQKRAAPQRELPRVESF"
     gene            complement(1644..2144)
                     /locus_tag="P5558_15320"
     CDS             complement(1644..2144)
                     /locus_tag="P5558_15320"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY25737.1"
                     /translation="MFRRKDNETTPGGETRAEAPLARPVAETKPMPQPSEAPRRGVEP
                     APAAAPTPAASADAGGKRLMVGQGISLSGEITSCDRLIVEGDVQVTLNETRAIEIAES
                     GRFTNGKASVEEAEISGVYEGELTVRNRLLIRATGRVKGTVRYGELELERGGRISGTI
                     SMIDDR"
     gene            2441..3448
                     /locus_tag="P5558_15325"
     CDS             2441..3448
                     /locus_tag="P5558_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRY25738.1"
                     /translation="MQVAAATGQKLDVLGIGNAIVDVLSHSDDELLTELGLEKGAMTL
                     VDEERSEFLYRRMGPGREVSGGSCANTIAAIASMGGSGAYIGRVRDDALGRIFAHDIR
                     AAGVRFDAAPQPDGPATARCLIFVTDDAQRTMQTYLGACVDLGPEDVDEELVRRAEVT
                     YFEGYLYDKPAAKAACQKAADICHAAGNKLALTLSDSFCVERWRAELKELVEGRVDIL
                     FANESEIKSLYEVASFDEALQQVRRHVGFAALTRGAKGSVVVRGDEVHVIDAVPPARL
                     VDTTGAGDLYAAGFLRGLTAGRDLATCGRLGSAAAAAIIGQYGARAAEPMTGLFAEMA
                     G"
     gene            3585..3827
                     /locus_tag="P5558_15330"
     CDS             3585..3827
                     /locus_tag="P5558_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25739.1"
                     /translation="MSTPKLALELNDLWAAEGRSYRLGRHGVAHLWAIWNERPDSGQP
                     LLFIVGSEERAVAQAHAWANPDATTRKADPPAIAAA"
     gene            complement(3840..4160)
                     /locus_tag="P5558_15335"
     CDS             complement(3840..4160)
                     /locus_tag="P5558_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25740.1"
                     /translation="MSLDFAARLGNTFVGAFGPSRRTLAAGPALPHGGLARRAVLALF
                     RLPARFVAALDRRQRERDALLRYAMAEGRGLGLGRQHVERAVLLTGRADMLFPSYFVN
                     ADRR"
     gene            complement(4407..5693)
                     /locus_tag="P5558_15340"
     CDS             complement(4407..5693)
                     /locus_tag="P5558_15340"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HRY25741.1"
                     /translation="MTDKPKYGFETLAVHAGAAPDPTTGARATPIYQTTSFVFDDVDH
                     AASLFNLQQFGNIYSRLTNPTVSVLEERVAALEGGSAACATASGHSAQFLTFLNLMSP
                     GDNIVSSSKLYGGSISQMNQTFPQFGWKVAYVDPREPENFRAAIDDRTKALFIESASN
                     PDGIVPDIEAIARIAHAAGIPLVVDNTCPTPYFCRPFEWGADVVVHSLTKYIGGQGNS
                     IGGMIVEGGKFDWAASGKFPLLTEPCAAYHGLRFYENFGAFAMTVRNKAIGLRDMGPC
                     LSPMNAFLILQGVETLPLRMERHVANADKVAAWLADHPQVTWVSYARLPQNRTPLVDK
                     YCPNGLGALFTFGVKGGYDMGVKVVNGCQMISHVANIGDTRSLIIHPSSTTHRQLTPE
                     QQTAAGAGPDVIRLSVGIETVDDIIADLDQALANAA"
     gene            6233..7738
                     /locus_tag="P5558_15345"
     CDS             6233..7738
                     /locus_tag="P5558_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25742.1"
                     /translation="MSDVMPRVILDELVALAVHHLGDGRAARDLVDQVLAEHPDLAAA
                     AVEAPSPAERLQAVDRLFGRVRWAVRDRLPGVASAATARPANAALPAHPPMPTAAPAA
                     APSPAPAPSPASDPSPAPGRPASPPWAPTRPQQAVPSDAAAPRSLAVPPAATIPQLEP
                     PRTFIPTIVAPGAEPEARVPGPAEFFGPPLPEVDPPLPEVDETPAAAPGPGALDSLPP
                     EPAGPEPPRHPAGSAVPAAASRPAAPQPRMRPRPAPPREESAPLAPLAPHPSHHREPP
                     PLQVEIVDDLYGIEAASRRRRRVLIGLAGVAAVAGAAWLVVLADLPQVSRDWEWPGSS
                     PASTTAEAPPAEESPPASLEALELQSPDVAADQPNGVPANGTRLALGLDQAPEPAAGP
                     TAAPAVPGEEAARVFIHYDADAASASAAAAALYARLAEAAAYATVVLRDVPYAIGSHR
                     VRYYHAADRPAAMALIERLGSPPGTDWQLQDFSNYRPLPVRGTLEVFVPTD"
     gene            complement(7760..8275)
                     /locus_tag="P5558_15350"
     CDS             complement(7760..8275)
                     /locus_tag="P5558_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRY25743.1"
                     /translation="MDEPLVYDDALIRRILKEVNRIAMVGASASEMRPSYFAMLYLLN
                     KGYEIDPVNPHHAGQTIQGRAVYPDLLSLPERPDMVQIFRRSEEVPPLVEAAIGIGAR
                     VIWMQLGVRHEAAAAQARAAGLDVIMDRCPKIEHGRLFGEIGWLGVNRGVLSAKRGVA
                     RQLSTTKGRLL"
     gene            complement(8289..9137)
                     /locus_tag="P5558_15355"
     CDS             complement(8289..9137)
                     /locus_tag="P5558_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY25744.1"
                     /translation="MTKTTGRFSNIVHPTDLGEAGRAAFAHGLRLAVAARGRFAVVHN
                     AGRELDDAAALEAFPGVRDTLASWGLLDLGAPTRSVEEVLGIRVSKADLGGGGTVETL
                     VDYIDRHGCDLVVLATHARDGLPRWLRGSVAAAIARRAAVPTLFLPHDARGFVEPRTG
                     ALRLANILMPIDSKPDPAPAIAAVTRLVEMLDAQAARLHLLHVGPAATAPEPAVAGWL
                     AQRLERHHREGPVVDAITAEADATDADLVVMTTEGRHGFLDALRGSTTEAVLRRANRP
                     LLAVPA"
     gene            complement(9146..9943)
                     /locus_tag="P5558_15360"
     CDS             complement(9146..9943)
                     /locus_tag="P5558_15360"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRY25745.1"
                     /translation="MQGSDETNLIEAEEGGVARLTLNRPKAFNSLDMALLDALHEALD
                     RIARSPTARVVVLAGHGKAFSAGHDLKEIGRDRRHAAIHRLFSRCSAMMLKLKDLPQP
                     VIAEVDGIATAAGCQLVAACDLAVCSAESRFATSGVKYGLFCSTPMVALSRNVPRKAA
                     MEMLLTGDFIDAAEALRLGLVNAVVPKAELAAATAGLCARLLDKPKDVLALGKRAFYR
                     QIELPIAEAYDLTTDVIVENALGDAFGAGLDAFVSKRPPVWPGQENG"
     gene            complement(10008..10709)
                     /locus_tag="P5558_15365"
     CDS             complement(10008..10709)
                     /locus_tag="P5558_15365"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRY25746.1"
                     /translation="MSGDDEEAPRFASPPCFMHELDPAYLMPPAGSKPSGDPAQEADV
                     RRWRKAERERLTGLRMALRAACRQAHDARVIDRLEAAIGPVRGRVVSAYWPFKAEPDL
                     RPLMKAIVGAGGRCALPVVVAKGQPLVFRAWAPGEKLVPGVWKIPVPLATAEAVVPDV
                     VIAPVIGFDAACYRLGYGGGFFDRTLASLAAKRPLVLGVGYALAAIPTIYPQWHDIPM
                     DLVVTEAGVVRPAGR"
     gene            complement(10706..11191)
                     /locus_tag="P5558_15370"
     CDS             complement(10706..11191)
                     /locus_tag="P5558_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRY25747.1"
                     /translation="MAGAGAGMEQAGERAAVVAEIIERTGIDEAMIDRLVRAFYAKVR
                     EDPLLAPVFEARIADWEPHLRRMVAFWSSVALMSGAYHGQPMERHARLPVDHRHFDRW
                     LALFKATAREVCPPAAAAHFVERAERIAMSLELGIATTCGRLPRRGERLRRPDAEVML
                     P"
     gene            11321..>11638
                     /locus_tag="P5558_15375"
     CDS             11321..>11638
                     /locus_tag="P5558_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25748.1"
                     /translation="MQPSTRFGLAGLALAAAASLATLPPRTAAAQDADAALETGIREI
                     ALAVGNTYVCAEGDAAQAAHRDMTRQLFDHILQDTGSNRAFLFAVSVGYGASVPKAEL
                     DCEK"
BASE COUNT         1682 a         4404 c         3914 g         1638 t
ORIGIN      
        1 tctggttcgg catcttcatc gtcgtcgtcg tcgagatggc gcagatcacc ccgcccgtcg
       61 gcttcaacct cttcgtcatg cagggcatca ccggccacaa catcctctac gtcgcccgca
      121 ccgccctgcc gttcttcctc ctcctcgtcg tcatggtcgg cctgctcgtg gccttccccg
      181 ggctcgccac ctggctgccc gagcagatgc gccagaccgc ggccggctga gtctccgggc
      241 ttgccgccgt cggcctgccg ccccaccttc gccgccatga gcgcgcagag aggccccttc
      301 gatcgtgccc ggcaccgtcg gcatcggcgc cgcttccccc gtggcccaga cttcctgcga
      361 agggccatcg aggagcggct gctcgaccgc ctcgacgaca tccgcacgcc cctcggccgg
      421 gtgctggtgc tcggtgccca ggacggcggc ctcgccgacg ccatccgcgg ccgacccggc
      481 gtcgatgcgg tcgtcaccgc cgacgagctg gccccggccg atgtcctgct cgacgaggag
      541 ctgctgccct tccgcgaagg cgccttctcc accgtgctga gcgccatgag cctgcactgg
      601 gtcaacgacc tgcccgggct cctcgcccag gtccgccact gcctcgccgc cgacggcctg
      661 ctgctcgcgg cctttcccgg cggcgagacc ctgcacgagc tgcgccaggc cctgctggcg
      721 gccgagctcg acgtcaccgg tggtgcgggg ctccgcctct cgccgatgat cgacgtgcgc
      781 gatgccggcg ccctcctgca gcgcgccggc ttcgccttgc cggtggcaga cgtcgagcgt
      841 ctcaccgtgc gctatgccga gccgcttcgc ctgctcatgg acctgcgcgc catgggcgag
      901 gcccaggcgc tgggcgaggg cgcccggggg tccctccacc gtccggtgct cggccggctc
      961 gacgccatcc tgcgggagcg cttcgccgat ccggacggca aggtgcgggc gagcttcgac
     1021 atcgtcacca tgaccggctg gaagccgcac gccagccagc ccaggccgct ccgccgcggc
     1081 tcgggcgagg tcagcctcgc caaggccctc ggcgtgccgc tcgaggtgct gcagggcaag
     1141 gcgaaaaagg cttagccgaa aggctcagaa gctctcgacg cgcggcagct cgcgctgcgg
     1201 tgccgcgcgc ttctggccct ggtcgagctg gcgcgccagg cgctcgcagc cggcagcggc
     1261 cttgccgttg ccgtagagcg gctcggggcg caggtcgtag tggacgacct cggcctgccg
     1321 gtcgggccgc gcgtcgacga agagatcgag gctgcagccg gcgaaggcat agcgccagta
     1381 ctgcacattg ccctcgacgc gcaccagcgc cggctcgccg aagacgctgc gcagatcgcc
     1441 ggagtccatg ccgacgagct cgacattgcg cgcgaggtcc ggcaccggcg cgggtggcgg
     1501 caggccgcgc cagagcggcg gcggggcgag ataggcgtcg atcggcaagg actccggccc
     1561 gccgctgcaa cccgcgagcg ccaccatggc gaagcccagg gaaaacatgc cggcggcgcg
     1621 ccgcaccgat cgagccatcg ccgctaccgg tcgtcgatca tcgagatggt gccggaaatg
     1681 cggccaccac gctcgagctc cagctcgccg tagcgcaccg tgcccttgac gcggccggtg
     1741 gcacggatca gcagacggtt gcgcaccgtc agctcgccct cgtagacgcc gctgatctcg
     1801 gcctcctcga cggacgcctt gccgttcgtg aagcggccgc tctcggcgat ctcgatggcc
     1861 cgggtctcgt tcagcgtgac ctgcacgtcg ccctcgacga tcagccggtc gcaggacgtg
     1921 atctcgcccg agaggctgat cccctggccg accatgagcc gcttgccgcc ggcatcggcg
     1981 ctcgcggccg gcgtcggcgc ggcagccggc gcgggctcga caccacggcg cggcgcctcg
     2041 gacggctgcg gcatcggctt ggtttcggcc acgggccgcg ccagcggcgc ctcggcccgt
     2101 gtctcgccgc ccggagtcgt ctcgttgtct tttctgcgaa acatcgatgg ttcctgcgtg
     2161 ctgcggttct ggaagtcgcc cgatgccgtg gcgggcggaa ggccgcgaag tagagcccgt
     2221 tctggaaagc ttcaaccgaa actgttcctc ggcggatcgc gaggattaga ccctgccggc
     2281 tcgggaagtc aaaccgaaag gaaccgccga ttgtcgcgac accggtcctt tcaatcgttc
     2341 tgggatgggt tgatgacttc ccgtcacgca cccgcactgg acgaacctct cgaacaggct
     2401 ctaaacgagc ggcggatcga caaacgggga atccaggcgc atgcaggtgg ctgccgcaac
     2461 cggccagaag ctcgatgtcc tcggcatcgg caacgccatc gtcgacgtgc tgagccacag
     2521 cgacgacgag ctgctcaccg agctcgggct cgagaagggg gcgatgacgc tcgtcgacga
     2581 ggagcgctcg gagttcctct atcgccgcat gggcccgggc cgcgaggtct cgggcggcag
     2641 ctgcgccaac accatcgccg ccatcgcgtc catgggcggc agcggcgcct atatcgggcg
     2701 ggtgcgcgac gacgcgctcg ggcggatctt cgcccacgac atccgcgccg caggcgtgcg
     2761 cttcgacgcc gcgccgcagc cggacggccc cgccacggcg cgctgcctga tcttcgtcac
     2821 cgacgacgcc cagcgcacca tgcagaccta tctcggcgcc tgcgtcgatc tcggccccga
     2881 ggacgtcgac gaggagctcg tccgccgcgc cgaggtcacc tatttcgagg gctatctcta
     2941 cgacaagccg gccgccaagg ccgcctgcca gaaggctgcc gacatctgcc acgccgccgg
     3001 caacaagctg gcgttgacgc tcagcgattc cttctgcgtc gagcgctggc gcgccgagct
     3061 caaggagctg gtcgaggggc gcgtcgacat ccttttcgcc aacgagagcg agatcaagag
     3121 cctctacgag gtcgcgagct tcgatgaggc gctccagcag gtccgccggc atgtcggctt
     3181 cgccgcgctg acccgcgggg ccaagggctc ggtcgtggtc cggggcgacg aggtgcacgt
     3241 catcgacgcc gtcccgccgg cgcggctggt cgacaccaca ggtgccgggg acctctacgc
     3301 cgccggcttc ctgcgcgggc tcaccgccgg ccgcgatctc gccacctgcg gccggctcgg
     3361 ctcggccgcg gccgccgcca tcatcggcca gtacggggcg cgcgctgcag agccgatgac
     3421 cgggctgttc gccgagatgg ccggctgagc gatcgcccgt cgcgcgatgt cgcgttcggg
     3481 gctaacctaa gggttgaaat tcgcggcgac ctgacgtagg tcgaagcctc cgccggagcg
     3541 ggcttcccgg gcccgctcgg gacgcttgcg agaggcatcc tgccatgagc acccccaagc
     3601 ttgccctcga gctcaacgat ctctgggccg ccgagggacg atcgtatcgc ctcggccgcc
     3661 acggcgtcgc ccatctctgg gcgatctgga acgaacggcc cgactccggc cagccgctcc
     3721 tcttcatcgt cggcagcgag gagcgtgcgg tggcccaggc ccacgcctgg gccaatcccg
     3781 acgccacgac caggaaagcc gacccgccgg ccatcgccgc cgcctgagcc gcgggacgat
     3841 cagcgccggt cggcgttcac gaaataggac gggaagagca tgtcggcacg gccggttagc
     3901 agcaccgccc gctcgacatg ctgccggccg aggccgagcc cgcggccttc cgccatggcg
     3961 tagcgcagca gcgcgtcacg ctcgcgctgg cgccggtcga gcgcggcgac gaagcgggcc
     4021 ggcaggcgga agagcgccag caccgcgcga cgggcgaggc cgccatgggg cagggccgga
     4081 cccgcggcga gcgtccgccg tgacgggccg aaggcgccga cgaaggtgtt gccgagacgg
     4141 gcggcgaagt cgagcgacat gggagttggc cctggagcga gtgacgtagc gccgcggata
     4201 tgggcgcgcc cgtgaccgcc gcaatggcgc aaaagagaag ccttctggcg tcgccgcgac
     4261 accgcccgcc gacgcaaatt ttcctgttgt ttgtcagata gttgggggag cgcgcgcgaa
     4321 gccctgcagg cacggcggaa ttgcatgcct gccaacgaga aagaacgggg cccgcaggcc
     4381 ccgttccaac gacaagaacg cctcgctcag gccgcgttcg cgagcgcctg gtcgaggtcg
     4441 gcgatgatgt cgtcgaccgt ctcgatgccg accgagaggc gaatgacatc gggcccggcg
     4501 cccgccgcgg tctgctgctc gggcgtgagc tgacggtgcg tcgtcgagga cggatggatg
     4561 atcagggagc gcgtgtcgcc gatattggcg acgtggctga tcatctggca gccgttcacc
     4621 accttgaccc ccatgtcgta gccgcccttg acgccgaagg tgaagagggc acccagcccg
     4681 ttggggcaat acttgtcgac caggggcgtg cggttctgcg gcagccgcgc ataggagacc
     4741 caggtgacct gcgggtgatc ggcgagccag gccgccacct tgtcggcatt ggcgacatgc
     4801 cgctccatcc tgagcggcag cgtctcgacc ccctgcagga tcaaaaacgc gttcatcggc
     4861 gacaggcacg ggcccatgtc gcgcagtccg atcgccttgt tgcgcacggt catcgcgaag
     4921 gcgccgaagt tctcgtagaa ccgcaggccg tgataggcgg cgcacggctc ggtcaggagc
     4981 gggaacttgc cagaagcggc ccagtcgaac ttgccaccct cgacgatcat cccgccgatc
     5041 gagttgccct gcccgccgat atacttggtc agcgaatgga cgacgacatc cgccccccat
     5101 tcgaacggcc ggcagaaata cggggtcggg caggtgttgt cgacgaccag ggggatgccg
     5161 gccgcatgcg cgatcctggc gatcgcctcg atgtcgggga cgatgccgtc cggattggag
     5221 gcgctctcga tgaagagggc cttcgtcctg tcgtcgatcg ccgcccggaa attctcgggc
     5281 tcgcgcggat cgacataagc gaccttccag ccgaactgcg ggaacgtctg gttcatctgg
     5341 ctgatcgagc cgccatagag cttggacgac gagacgatgt tgtcgcccgg gctcatcagg
     5401 ttgaggaagg tcaaaaactg cgccgaatgg cccgacgcgg tggcgcaggc agcgctgccg
     5461 ccctcgagtg ccgcgacccg ctcctcgagc accgagaccg tggggttcgt gagcctcgaa
     5521 tagatattgc cgaactgctg caggttgaac agcgaggcgg cgtggtcgac atcgtcgaag
     5581 acgaaggacg tcgtctggta gatcggcgta gcccgcgcac cggtcgtcgg gtccggcgcc
     5641 gcgccggcat gcaccgccag ggtctcgaaa ccatatttgg gtttgtcggt cattgtcgcg
     5701 ttccctctcc attggcacgg ccattggccc cctcgtatag ccgggtcggc gacagcgcca
     5761 agcgacattc gtcacatcgc cggtttccac ccgaccgctg aaacggcgtg gcggccatgc
     5821 ccgttgcagg catgcggcca gaagaaggcg cccggaagag ggcgcatcga gcgatcatga
     5881 atgcgcccgt aaggcagtcc gggacgtttt cagggtgcat ccctcggcgg cctgccggca
     5941 gcgtcgccct cggcaatcgg acgagcgcca ttaggccctt catccgccct ctggctgctg
     6001 cggttgatcc gaggcggact cgacatacct cgcgaggagc ccggccgaag gtcttgcgga
     6061 tggcgcgctt ccccgcgtac cagatggcgt gcaggcaggc cgctcccgca cgcggccggc
     6121 agtcatgccg ccgtgagctg tgctagactt ggtgtgtcag caagatgccg ggccggccgc
     6181 gccgtatcgg cgatgcccgg ccggggcttc tgggggtgac aggtaggcct tgatgtccga
     6241 cgtgatgccg cgtgtgatcc tcgacgagct ggtggcgctc gccgtgcacc atctcggtga
     6301 cggccgggcg gccagggacc tcgtcgacca ggtgctggcc gagcatccgg atctcgccgc
     6361 cgccgccgtc gaggcaccct cgccggccga gcggctgcag gcggtcgacc ggctgttcgg
     6421 tcgcgtcaga tgggcggtcc gcgatcggct gcccggtgtc gcctcagccg ccaccgcccg
     6481 gcccgccaat gccgccttgc cggcccatcc gcccatgccg accgctgctc ctgcggcagc
     6541 cccgtcaccg gcgccggccc catcgccggc gtcggacccg tctcctgcgc cgggccggcc
     6601 cgcatccccg ccctgggcgc cgacccggcc gcagcaggcc gtgccgtcgg atgccgccgc
     6661 cccgcgctca ctggccgtgc ccccggcggc gacgattccc cagctcgagc cgccccgcac
     6721 cttcatcccg accatcgtgg cccccggggc cgagcccgag gccagggtgc ccgggccggc
     6781 cgaattcttc ggccccccct tgcccgaggt cgacccgccc ttgcccgagg tcgacgagac
     6841 cccggccgcc gcacccgggc ccggggcgct cgactccctt ccgcccgagc cggccgggcc
     6901 cgagccgccg cggcatccgg caggttcagc cgtgcccgcc gccgcttcgc ggccggccgc
     6961 gccgcagccg cggatgcggc cacgcccggc gccgccacgg gaggagtccg cgccgctcgc
     7021 gcctttggca ccgcatccgt cgcaccaccg cgagccgccg cccttgcagg tcgagatcgt
     7081 cgacgacctc tacggcatcg aggcggcctc gcgccgccgc cgccgtgtcc tcattggcct
     7141 cgccggggtc gccgccgtcg ccggggccgc ctggctggtg gtgctggccg atctgccgca
     7201 ggtctcgagg gactgggagt ggcccgggtc gtcgcccgca tcgacgaccg ccgaggcccc
     7261 cccggccgag gagagcccgc cggcatcgct cgaggcgctc gagctccaat cgcccgacgt
     7321 cgccgccgac cagccgaacg gggtgccggc caatggcacg cggctcgccc tcgggctcga
     7381 ccaggcgccg gagccggccg ccgggccgac cgccgctccg gccgtacccg gggaggaggc
     7441 ggcccgggtc ttcatccact atgatgccga cgccgccagc gccagcgccg ctgccgcggc
     7501 cctctatgcc cggctcgccg aggccgccgc ctacgcgacg gtcgtgctgc gcgacgtgcc
     7561 ttacgccatc ggcagccacc gcgtgcgcta ctaccatgcg gcggaccgcc ccgcggcgat
     7621 ggcgctgatc gaacggctcg gcagcccgcc ggggaccgac tggcagctgc aggatttcag
     7681 caactaccgg cccctgccgg tgcgcggcac gctcgaggtg ttcgttccga ccgactgagc
     7741 tcccgggcca gccggccgct cagagcagcc ggcccttggt cgtgctgagc tgccgggcga
     7801 cgccgcgctt cgccgagagc acgccccggt tcacgccgag ccagccgatc tcgccgaaga
     7861 gccgcccgtg ctcgatcttc gggcagcgat ccatgatcac gtcgagcccc gccgcgcgcg
     7921 cctgcgccgc cgccgcctcg tggcgcacgc cgagctgcat ccagatcacc ctggccccga
     7981 tcccgatcgc cgcctcgacc aagggcggca cctcctcgga gcggcggaag atctgcacca
     8041 tgtccggccg ctccggcagg ctcaggagat cgggatagac cgcccgcccc tggatcgtct
     8101 ggccggcatg atgcgggttc accgggtcga tctcgtagcc cttgttcagg agatagagca
     8161 tggcgaagta tgacggccgc atctcgctcg ccgaggcgcc gaccatggcg atccgattca
     8221 cctccttcag gatgcggcgg atcagggcgt cgtcgtagac cagcggctcg tccatcagca
     8281 tgcggccctc aagccggcac cgcgagcagc ggccggttgg cccgccgcag caccgcctcg
     8341 gtggtcgagc ccctgagcgc atcgaggaag ccgtgccggc cctcggtcgt catcaccacg
     8401 agatcggcgt ccgtcgcatc ggcctcggcg gtgatcgcgt ccaccaccgg cccctcgcgg
     8461 tgatggcgct cgagccgctg cgccagccag cccgccaccg ccggctcggg tgccgtcgca
     8521 gccgggccga catgcagcag atgcagcctc gcggcctgcg catcgagcat ctcgaccagc
     8581 cgggtcaccg cggcgatggc cggcgccggg tcgggcttgc tgtcgatcgg catcaggatg
     8641 ttggcgagcc tgagagcgcc ggtcctcggc tcgacgaagc cgcgcgcgtc atggggcagg
     8701 aagagcgtcg gcacggcggc acgccgcgcg atcgccgcgg ccaccgagcc cctgagccag
     8761 cgcggcaggc cgtccctcgc atgggtggcc agcaccacga ggtcgcagcc atgccggtcg
     8821 atatagtcga cgagggtctc gaccgtgccg ccgccgccga gatcggcctt gctcaccctg
     8881 atgccgagca cctcctcgac gctccgggtg ggcgcgccca ggtcgagcag gccccagctc
     8941 gccagcgtgt cgcgcacgcc cggaaaggcc tcgagcgccg ctgcgtcgtc gagctcgcgc
     9001 ccggcattgt ggaccaccgc gaagcgaccc ctggccgcca cggcgagcct gaggccgtgc
     9061 gcgaaggcgg cccgccccgc ctcgccgagg tcggtcggat gaacgatgtt cgagaagcgt
     9121 cccgtggtct tggtcatgtc ctggatcatc cgttttcctg gccgggccag actggcggcc
     9181 gcttgctgac gaaggcatcg agaccggcac cgaaggcgtc gccgagcgca ttctcgacga
     9241 tcacgtcggt ggtcaggtca taggcctcgg cgatcggcag ctcgatctgc cggtagaagg
     9301 cccgcttgcc cagggccagc acgtccttcg gcttgtcgag cagccgggcg cagagacccg
     9361 ccgtcgccgc cgccagctcc gccttcggca ccacggcgtt gacgagaccg agccgcaagg
     9421 cctccgccgc gtcgatgaaa tcgcccgtca gcagcatctc catcgccgcc ttgcgcggca
     9481 cgttccgcga cagcgccacc atcggcgtcg agcagaagag cccgtacttc acgcccgagg
     9541 tggcgaaccg gctctccgcg ctgcagaccg cgaggtcgca ggcggcgacg agctggcagc
     9601 ccgccgccgt ggcgatgccg tccacctcgg cgatcaccgg ctgcggcagg tccttgagct
     9661 tcagcatcat cgccgagcag cggctgaaca ggcgatggat cgccgcgtgc cgccggtccc
     9721 gcccgatctc cttcagatca tgcccggccg agaacgcctt gccgtgcccc gccagcacca
     9781 ccacccgcgc cgtcggcgac cgggcgatcc ggtcgagcgc ctcgtgcagc gcgtcgagca
     9841 gcgccatatc caggctgttg aaggccttcg gccggttcag cgtcagccgg gccacgccgc
     9901 cctcctccgc ctcgatcagg ttcgtctcgt cgctgccctg catgcccgtc ccctcctggc
     9961 cgccgcgcgg aagctacagg caaaccccgc cccgggcacg cccgaattca tcgccccgcc
    10021 ggccgcacca cgcccgcctc ggtgaccacg agatccatcg ggatgtcgtg ccactgcggg
    10081 tagatggtgg ggatcgccgc cagcgcatag cccaccccca gcacgagcgg ccgcttcgcc
    10141 gccagcgaag ccagcgtccg gtcgaagaac ccgccgccat agcccagccg atagcacgcc
    10201 gcatcgaagc cgatcaccgg ggcgatcacc acatccggca ccaccgcctc ggccgtcgcc
    10261 agcggcaccg ggatcttcca cacccccggc accagcttct cccccggcgc ccaggcgcga
    10321 aagacgagcg gctgcccctt cgccaccacc accggcaagg cgcagcgccc gccggccccg
    10381 acgatcgcct tcatcagcgg ccggagatcc ggctccgcct tgaacggcca ataggcgctg
    10441 accacgcgcc cccgaaccgg gccgatcgcc gcctccagac ggtcgatgac ccgcgcgtca
    10501 tgcgcctgcc ggcacgctgc ccgaagcgcc atgcgcagcc ccgtcagccg ctcgcgctcc
    10561 gccttccgcc agcgccgcac atcggcctcc tgcgccgggt cccccgacgg cttcgagccg
    10621 gcgggcggca tcaggtaagc cgggtccagc tcgtgcatga agcagggcgg cgaggcaaag
    10681 cggggcgcct cctcgtcgtc gccgctcacg gcagcatcac ctccgcgtcc ggccgccgca
    10741 ggcgctcgcc ccgccgtggc agccgcccgc aggtcgtggc gatgccgagc tccaggctca
    10801 tggcgatgcg ctccgcccgc tcgacgaaat gcgccgccgc cgccggcggg cagacctcgc
    10861 gggccgtcgc cttgaacagc gccagccagc ggtcgaaatg gcgatgatcc accggcagcc
    10921 gcgcatggcg ctccatcggc tggccgtgat aggcgccgct catgagcgcc accgacgacc
    10981 agaaggcgac catgcgccgg agatgcggct cccagtccgc gatccgcgcc tcgaacaccg
    11041 gcgccagcag gggatcctcg cgcaccttcg catagaacgc gcgcaccagc cggtcgatca
    11101 tcgcctcgtc gatgcccgtc cgctcgatga tctccgccac caccgccgcc cgctcgcccg
    11161 cctgctccat cccggccccg gctcccgcca tgaataggta ttcgaaatgc ctttttagca
    11221 gccgccaacc cgaccggcaa cgctggacga ccggccgggc ttgcgtcatc ctgaccacgg
    11281 cggtgcactc gccggcacgg cgcgacaggg agagacgacc atgcaaccaa gcacacgatt
    11341 cggcctggcg ggcctcgccc tcgcggccgc cgccagcctc gccaccctgc caccccgcac
    11401 ggccgcggcc caggacgccg atgccgccct cgagaccggc atccgcgaga tcgccctcgc
    11461 cgtcggcaac acctatgtct gcgccgaggg cgacgcggcc caggccgccc accgcgacat
    11521 gacgcgccag ctcttcgacc acatcctcca ggacacgggc tccaaccggg ccttcctctt
    11581 cgccgtctcg gtcggctacg gcgcttccgt gccgaaggcc gagctcgact gcgagaag
//
LOCUS       DAOTFI010000152        11621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6597_length_11621_cov_6.514698, whole genome shotgun sequence.
ACCESSION   DAOTFI010000152 DAOTFI010000000
VERSION     DAOTFI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_11621_cov_6.514698"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="P5558_15380"
     CDS             complement(<1..364)
                     /locus_tag="P5558_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25749.1"
                     /translation="MGWSSFWPALATVAIACGAQGAELRLAALDTPELRAIQDLSGEW
                     EDATGHRLAWTFLDAQAIGERVRQQALVPAADGAAADRLDIATLGPLQLDIWAEQGWL
                     APLPVGGSFGAPLVTVTTD"
     gene            complement(465..1685)
                     /locus_tag="P5558_15385"
     CDS             complement(465..1685)
                     /locus_tag="P5558_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRY25750.1"
                     /translation="MPSALADIRILDLTRILAGPTCTQLLGDLGCDVLKIEKPGAGDD
                     TRKWGPPYAPAKGGGDTNESAYYLAANRNKRSVAIDIATAEGQALIRSLLASSDVLVQ
                     NFKAGTLARYGLDHDSLKGQFPGLVYCSISGFGQTGPYADRAGYDYLAQGYGGIMSLT
                     GAPDGEPMKVGVGIADIMCGMYASSAILAALHHKARTGEGQHIDLALLDTQVAWLANE
                     GVNYLLSGKVPQRLGNEHPNIVPYKRFDTADGEVVLAAGNDAQFRRFCRFAGAEELAH
                     DPRFSGNPDRVRNRTALYALLVPIFRQKTTSVWVEGLTALGVPCGPVNDLAQVFADPQ
                     VQHREMQIDMPHPLTSSGTVPLLGNPIKMSATPPAYAKPPPFLGQHTDEVLRELLGLD
                     DAELDRLRRQGVIG"
     gene            complement(1759..2556)
                     /locus_tag="P5558_15390"
     CDS             complement(1759..2556)
                     /locus_tag="P5558_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25751.1"
                     /translation="MARSIPDSRWATAKSPVATAPAGGGARVLERLVLVLPILLLPHV
                     GTPDDILRVGCLVAAVLAIDLGLDTGWPATPAALGIAIGAGLGLAVGPGAAWPLALFA
                     AVAMLERTARADQDWLARIGLRALAAALLVDLALVVLDLERSLDWLALGAGISLALAA
                     AERLRVEAAAAATRDAAADDRRLGALETALAVGLVVAIGAHAALLAGDPALAPLARAG
                     GYLVLPFFALGLLRFGWLALARREGSDPLGGALLAGWALAASLLLEP"
     gene            2803..3279
                     /locus_tag="P5558_15395"
     CDS             2803..3279
                     /locus_tag="P5558_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HRY25752.1"
                     /translation="MIRLSKLADYGIVIMTHLARPGRVQESAQEIALATQVPSAMASK
                     ILKSLARAGLLRSQRGVKGGYELARLPGAVSMAEIIEALDGPIALTDCVDGAHGDCTI
                     EALCPARTNWDRINTAIRGALDSVSLDEMASAIPTAFLTLEEQGRGEAPRRALAGP"
     gene            3351..4796
                     /gene="sufB"
                     /locus_tag="P5558_15400"
     CDS             3351..4796
                     /gene="sufB"
                     /locus_tag="P5558_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRY25753.1"
                     /translation="MATALETVQDYAAKAYKYGFSTAIESDMAPKGLNEDIVRLISAK
                     KNDPEWLAEWRLEAFRRWLTMEEPTWQKVHYPPIDYQDVHYYAAPKIKERPKSLDEID
                     PEIRSTYDKLGIPIKEQEILAGVAVDYVWDSVSVATTFRAKLHELGIIFCSISEAVHD
                     HPELVRKYLGSVVPAGDNFFAALNAAVFTDGSFVYVPKGVRCPMELSTYFRINEMNTG
                     QFERTLIIADEGSYVSYLEGCTAPQRDENQLHAAVVELIAHDDAEIKYSTVQNWYPGN
                     AEGKGGIYNFVTKRGACRGKRSKISWTQVETGSAITWKYPSCILQGDDSVGEFYSVAV
                     TNNAQQADTGTKMIHIGKRTRSRIVSKGISAGRADQTYRGLVRMQKGAEGARNHTQCD
                     SMLIGDKCGAHTVPYIEIKNRTAQAEHEATTSKISDDQLFYCRSRGIAEEDAVALIVN
                     GFCREVMNELPMEFAVEARKLLGISLEGSVG"
     gene            4844..5599
                     /gene="sufC"
                     /locus_tag="P5558_15405"
     CDS             4844..5599
                     /gene="sufC"
                     /locus_tag="P5558_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRY25754.1"
                     /translation="MLKITDLHVAVDGGSKPVLKGLTLEVKPGEVHAIMGPNGSGKST
                     LSYVLAGRDGYAVTGGSVRFQDADLLELEPEERAAAGIFLAMQYPVEIPGVANNYFLR
                     QSLNALRRARGEPEVSAAEFIKKLKAVAERVGVDPELLKRDVNAGFSGGEKKRNEILQ
                     MVMLEPKLCVLDETDSGLDIDALKTVADGVNSLRSPDRGMIVITHYQRLLDYIVPDFV
                     HILADGRIRKTGGKELALALEEQGYEHLLGEAA"
     gene            5605..6960
                     /gene="sufD"
                     /locus_tag="P5558_15410"
     CDS             5605..6960
                     /gene="sufD"
                     /locus_tag="P5558_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRY25755.1"
                     /translation="MNAPARAIRSRYVAPIPAFADAFTAARSSLPGKEAPAIQDWRQL
                     HFDRFLETGLPTPRVEQWKYTNIARAANVPMALATRPNVGLGDVQRYFTGGPLARRLI
                     FVNGHLASELSHVRGLPQGVRVESLGKLLQDEPEWSARALGGPAEPHSFADLNAAFLQ
                     SGAWIELGDGAVLEHPLQLLFLTVGQPAAVMAHPRIVVRLGKEARLHLIETHIGLAPG
                     HCLTNLVGHYELGEKARLTHDRLQLGSAGTSFVGLNRQRLAERAELKQTLATLGGALV
                     RNETDLHLVGSFVEAQLNGVYMPIGEEHVDNVIRIHHEAPDGHSDQFYKGVMDDRAHA
                     AFAGKIIVHQDAQRTNAFQRNDNLLLSDDAEISSKPELEIYADDVKCSHGATCGELDR
                     TALFYLRSRGLDRAQAESILTFAFAAEVIERFVDPAVRKLARECLVARTPGGAGLSDL
                     L"
     gene            6957..8210
                     /locus_tag="P5558_15415"
     CDS             6957..8210
                     /locus_tag="P5558_15415"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HRY25756.1"
                     /translation="MNALATRPAPHAALDIEAVRAEFPILGTTMQGQPLVYLDSGASA
                     QKPRLVIDTLTEVYSAGYANIHRGVYELSQVASARHDEARRKLQRFIGAADWREIVFT
                     RNATEAINLVAQCFTAPLLGPGDEILVTEMEHHANIVPWQMVAEPRGARVVAAPVTDA
                     GELDLDAFEGLIGERTRMIAVAWISNVLGTVNPVAELVAMARRHGVPILIDAAQAVQH
                     TSVDVAALDADFLAFSGHKMYGPSGVGVLWGKAAHLDAMPPYQGGGDMIERVSFQGTT
                     WNETPFKFEAGTPDIAGIIALGAAVDFIEGLGIEAIAAHEAALLAYATRALQQVPGLR
                     LIGTARDKASVLAFELAGAHPQDIGMLLDLDGIAIRTGHHCAMPLHERLGMSASARAS
                     IGVYNNEADIDALVRSLGRVAEMFQ"
     gene            8225..8662
                     /locus_tag="P5558_15420"
     CDS             8225..8662
                     /locus_tag="P5558_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRY25757.1"
                     /translation="MDDNGVGRGADRGADQGLDRAEAFLGAAGGEEPAAASPERMAEV
                     DRLGEAIIAQLMTIFDPEIPVNIYELGLIYKIDVHDGNTVTVDMTLTSPNCPVAESLP
                     MEVQHKVEQVEGIEGCEVRIVWEPTWHPSMMTEEAQLELGLIY"
     gene            8719..9663
                     /locus_tag="P5558_15425"
     CDS             8719..9663
                     /locus_tag="P5558_15425"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043745147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRY25758.1"
                     /translation="MIDLTFRQKHLLGIEGLQPWEIADLLDCADRYVDLNRRPAKKLQ
                     LLDGLTQINLFFESSTRTLTSFELAGKRLGADVVNMAVQGSSIKKGETLIDTAMTLNA
                     MRPDVLVVRHQDSGAVSLLARHVNCSVINAGDGSHEHPTQALLDALTIRRRKGRLEGL
                     TVAICGDVRHSRVARSNILLLLAMGASVRLIGPPTLLPRAFSTFGCELFTDMAKGLQG
                     CDIVMMLRLQNERMQGSYVPSVREYFHLYGLTREKLAHAAPDALIMHPGPMNRGVEID
                     GVIADDIDRSAILDQVEMGVAVRMACLDLLTRDWRAAA"
     gene            9660..10943
                     /locus_tag="P5558_15430"
     CDS             9660..10943
                     /locus_tag="P5558_15430"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRY25759.1"
                     /translation="MRPLLFTAVRLLDPATGLDAPGELLVQQGRIAAFGPSVDRPDGV
                     EIVAGEGACLCPGLVDMRVHFGEPGTEHKERLASGIRAAAAGGVTSVAVLPNTRPPID
                     DPAMLEFVARRARQLKSVKVYPYAALTKGLEGRELAELALLKEAGAVAFTDAEQALAD
                     ARVMQRALAYASALDLLVVQHPEEPSLASGGVVNEGAIAVRLGLRGIPAIAEVMMVER
                     DLHLVRATGARYHVAHISTRAAIDAVRRAKAEGLRVTCETTPHHLALNELEVEGYRTF
                     AKVSPPLRAEADREAVVEGLVDGTIDVIASDHSPQDQDSKRLPMAQAEFGVIGVETML
                     PVSLRLVHEGQMSLLDLLRKLTVAPADLLGLEAGRLAQGAAADLTLFDAERPWKITEA
                     GLSSLAKNTAFEGRLVQGRVLLTMVDGRVVHRFTA"
     gene            11042..11521
                     /locus_tag="P5558_15435"
     CDS             11042..11521
                     /locus_tag="P5558_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25760.1"
                     /translation="MAATDRPLFGADRLLTMLLAGAVATAAFDLFGQSLSPGLGFANL
                     APVPLANNVIQVLLGAPWAPGAHALHYLAGLVGYPLGWMLIARPIALRLTPTWPWWLT
                     SAIYGVGLWIFALFIMAHLVAGQPAFLGFIGITWVALVGHVVYAIAAAAVVQWREPG"
BASE COUNT         1844 a         3978 c         4074 g         1725 t
ORIGIN      
        1 cgtcggtggt gacggtgacc agcggagctc cgaagctgcc gccgaccggt agcggtgcca
       61 gccagccctg ctcggcccag atgtcgagct gcagcgggcc gagggtggcg atatcgagcc
      121 ggtcggcagc agcaccgtcg gccgccggca cgagggcctg ctggcgcacc cgttcgccga
      181 tcgcctgcgc gtcgaggaag gtccaggcca gtcgatgccc ggtagcatcc tcccattcgc
      241 ccgagaggtc ctggatggcg cgaagctcgg gcgtgtcgag cgcggcgagc cgcagctcgg
      301 cgccctgcgc gccgcaggcg atggccaccg tggccagtgc cggccagaag gagctccagc
      361 ccatcacgtt cccctactac cgaaggtagt tggtgcaacc ggtttccgga ccgtatgaag
      421 ttgattctag agcgtcatcc cgttgccgtc cagttcgggt ccgatcagcc aatcaccccc
      481 tgtcggcgca gccggtcgag ctcggcgtcg tcgaggccca gaagctcgcg cagcacctcg
      541 tcggtatgct ggccgagaaa gggcggcggc ttggcatagg ccggcggcgt cgccgacatc
      601 ttgatgggat tgccgagcag cggcacggtg cccgaactgg tcagcggatg cggcatgtcg
      661 atctgcatct cgcggtgctg gacctgcggg tcggcgaaga cctgtgccag gtcgttgacc
      721 gggccgcagg gcacgccgag tgccgtcagg ccctctaccc atacggacgt cgtcttctgc
      781 cggaagatcg gcacgagcag ggcataaagg gcggtgcggt tgcgcacgcg gtcgggattg
      841 cccgagaagc gcgggtcatg ggccagctcc tcggccccgg cgaagcggca gaagcggcgg
      901 aactgggcgt cgttgccggc ggccagcacc acctcgccgt cggcggtgtc gaaccgcttg
      961 taggggacga tgttcgggtg ctcgttgccg agcctttgcg gcaccttgcc ggagagcagg
     1021 tagttcaccc cctcgttggc gagccaggcc acctgggtgt cgagcagggc gaggtcgata
     1081 tgctggccct cgccggtccg cgccttgtgg tgcagggcgg cgaggatggc gctcgacgca
     1141 tacatgccgc acatgatgtc ggcgatgccg acgccgacct tcatcggctc gccgtcggga
     1201 gcgccggtca ggctcatgat cccgccatag ccctgggcca ggtagtcgta gccggcccgg
     1261 tcggcgtagg ggccggtctg gccgaagccg ctgatcgagc agtagacgag gcccgggaac
     1321 tggcccttca gcgagtcgtg gtcgagcccg tagcgggcga gcgtgcccgc cttgaagttc
     1381 tgcaccagca cgtcggagct ggcgagcagc gagcggatga gcgcctggcc ctcggcggtc
     1441 gcgatgtcga tcgccaccga gcgcttgttg cggttggcgg cgagatagta ggcgctctcg
     1501 ttggtatcgc cgccgccttt ggccggggca tagggcgggc cccatttgcg cgtgtcgtcg
     1561 cccgcacccg gcttctcgat cttgagcacg tcgcagccga ggtcgccgag gagctgggtg
     1621 caggtcggac cggcgagaat tcgggtcagg tcgagaatgc ggatatcggc gagcgctgac
     1681 ggcatggcta cctcccggaa cggctgcgca ggaggtagcc cggccggcac cggtgcggca
     1741 agggcgagcc gtgccggctc agggctccaa gaggaggctc gccgccagag cccagccggc
     1801 caggagggca ccgccgagcg gatcggaccc ttcccggcgt gccagcgcca gccagccgaa
     1861 gcgcaggagg ccgagggcga agaacggcag gaccaggtag ccgccggcgc gggccagggg
     1921 agccagcgcc gggtcgccgg cgagcagcgc cgcgtgggcg ccgatggcaa cgaccaggcc
     1981 cactgcaagg gccgtctcca gggcgccgag ccggcggtcg tccgctgccg cgtctctcgt
     2041 ggcggctgcg gcagcctcga cccgcagccg ctcggcggcg gcgagggcga ggctgatacc
     2101 ggcaccgagc gccagccagt cgaggctccg ctcgagatcg aggacgacga gcgccaagtc
     2161 gacgagcagg gcggcggcga gggcgcgcag gccgatgcgc gccagccagt cctggtcggc
     2221 acgggcggtg cgctcgagca tggcgacagc ggcgaagagc gccagcggcc aggcggcgcc
     2281 cgggccgacg gcgaggccga gcccggcgcc gatggcgatg cccagcgctg cgggcgtggc
     2341 gggccagccc gtgtcgaggc ccaggtcgat ggccagcacg gcggcgacga ggcaaccgac
     2401 ccgcaggatg tcgtccggcg tgccgacatg gggcagcagg aggatcggca ggacgaggac
     2461 cagccgctcg aggacgcggg cgccaccgcc ggccggagcc gtcgcgacgg ggctcttggc
     2521 cgttgcccat ctgctgtcgg gtatgcttcg cgccatcggc cgccgctccg tccatgccgc
     2581 ccacgaccgt tcgcggggct cgcccggacc ctacgcaccg gtggttaccg tccggttaac
     2641 cctcatgaga ggcgatgccg ccttgacagt gggagccaat gggccaaatt gcggaatgtg
     2701 gaccgatatg gtcccggttc gttcacagcc tcgcgtagcg gcccggtgga agcgggtcgg
     2761 ctggcctcgg ggcctgctgg atatggggac gggtggtcgg ccttgatcag actgagcaag
     2821 ctcgcggact atggcattgt catcatgacg catctggcgc gcccgggccg cgtgcaggag
     2881 agtgcgcagg agatcgcgct ggcgacccag gtgccgtcgg cgatggcgag caagatcctg
     2941 aagtcgctgg cgcgcgccgg cctgctgcga tcgcagcgcg gggtcaaggg cggctacgag
     3001 ctggcccggc tgccgggtgc tgtctccatg gccgagatca tcgaggccct tgacgggccg
     3061 atcgcactca ccgactgcgt cgacggcgcg catggcgact gcacgatcga ggcgctctgc
     3121 ccggcgcgca ccaactggga ccgcatcaac acggcgatcc ggggagcgct cgacagcgta
     3181 tcgctcgacg agatggcgag cgccatcccg acggccttcc tcaccctcga ggagcagggc
     3241 cggggcgagg cgccgcgccg ggccctggct ggtccctagg ttttcgggcg gccacggaag
     3301 cgccgggccc cgtgggccgg gcgagacagt ttttcttgga gctttgacga atggcgacgg
     3361 cactcgagac ggttcaggac tatgcggcga aggcctacaa gtacggcttt tcaacggcga
     3421 tcgagtccga catggcgccg aagggcctga acgaggacat cgtccgcctg atctcggcca
     3481 agaagaacga tcccgagtgg ctcgccgagt ggcggctcga ggcctttcgc cgctggctca
     3541 ccatggagga gccgacctgg cagaaggtgc actacccgcc gatcgactac caggacgtgc
     3601 actactacgc cgcgccgaag atcaaggagc ggccgaagag cctcgacgag atcgatcccg
     3661 agatccgctc gacctacgac aagctcggca ttccgatcaa ggagcaggag atcctggccg
     3721 gggtcgccgt cgactatgtc tgggacagcg tctcagtggc gaccaccttt cgcgccaagc
     3781 tgcatgagct cggcatcatc ttctgctcga tctcggaggc ggtgcacgac catccggagc
     3841 tggtgaggaa gtacctgggc tcggtggtgc cggcgggcga caacttcttc gccgcgctga
     3901 atgcggcagt cttcaccgac ggcagcttcg tctacgtgcc caagggcgtg cgctgcccga
     3961 tggagctctc gacctatttc cgcatcaacg agatgaacac cggccagttc gagcggacgc
     4021 tgatcatcgc cgacgagggg tcctacgtgt cctacctcga gggctgcacg gcgccgcagc
     4081 gcgacgagaa ccagctccat gcggcggtgg tcgagctgat cgcccacgat gacgccgaga
     4141 tcaagtactc gacggtgcag aactggtacc ccggcaatgc cgagggcaag ggcggcatct
     4201 acaacttcgt gaccaagcgc ggcgcctgcc gcggcaagcg ctcgaagatc tcatggacgc
     4261 aggtcgagac cggctcggcg atcacctgga aatacccgtc ctgcatcctt cagggcgacg
     4321 attcggtcgg cgagttctac tcggtcgcgg tgaccaacaa tgcccagcag gccgacaccg
     4381 gcaccaagat gatccacatc ggcaagcgca cgcgctcgcg aatcgtcagc aagggcatct
     4441 ccgccggccg cgccgaccag acctatcgag gcctggtgcg gatgcagaag ggggccgagg
     4501 gtgccaggaa ccacacgcag tgcgattcga tgctgatcgg cgacaagtgc ggcgcccaca
     4561 cggtgccgta catcgagatc aagaaccgca cggcgcaggc cgagcacgag gcgacgacca
     4621 gcaagatcag cgacgaccag ctcttctact gccgcagccg cggcatcgcc gaggaggacg
     4681 ccgtcgccct catcgtcaac ggcttctgtc gcgaggtgat gaacgagctg cccatggagt
     4741 tcgccgtgga ggcgcgcaag ctgctcggca tctcgctcga gggtagcgtc ggctgatggc
     4801 caagaaaaac gtatcggatt cagacggttc gagggttgtt tcgatgttga agatcactga
     4861 cctgcacgtc gccgtcgatg gcggctcgaa gccggtcctc aagggcctca cgctcgaggt
     4921 gaagccgggc gaggtgcatg ccatcatggg accgaacggc tcgggcaaga gcacgctctc
     4981 ctatgtgctc gccggtcgcg acggctacgc ggtgacgggc ggctcggtgc gctttcagga
     5041 tgcggatctg ctggagctgg agcccgagga gcgggcggcc gccggcatct tcctcgccat
     5101 gcagtatccg gtcgagattc cgggtgtcgc gaacaactat ttcctccgcc agtcgctcaa
     5161 cgcgctgcgc cgggcgcgcg gcgagccgga ggtcagcgcc gccgagttca tcaagaagct
     5221 caaggcggtg gccgaacggg tgggcgtcga tcccgagctg ctcaagcgcg acgtcaatgc
     5281 cgggttctcg ggcggcgaaa agaagcgcaa cgagatcctg cagatggtga tgctcgagcc
     5341 gaagctctgt gtgctcgacg agaccgacag cgggctcgac atcgacgcgc tcaagaccgt
     5401 ggccgacggc gtcaactcgc tgcgcagccc cgatcggggg atgatcgtca tcacccacta
     5461 ccagcgcctg ctcgactata tcgtgccgga cttcgtccac atcctggccg acggccggat
     5521 ccgcaagacc ggcggcaagg agctggcgct ggcgctcgag gagcagggct acgagcacct
     5581 gctcggcgag gcggcgtgac ggccatgaat gctcctgcac gggccatccg gtcccgctat
     5641 gtggcgccga tccctgcctt cgccgacgcc ttcacggcgg cacggtccag cctgcccggc
     5701 aaggaagcgc cggcgatcca ggactggcgg cagcttcatt tcgaccgctt ccttgagacc
     5761 ggcctgccga cgccccgggt cgagcagtgg aaatacacca acatcgccag ggccgcgaac
     5821 gtgccgatgg cgctggcgac ccggccgaat gtcgggctcg gcgacgtcca gcgctatttc
     5881 accggcggcc ccttggcccg ccggctgatc ttcgtcaacg ggcacctcgc gagcgagctc
     5941 agccatgtgc gcggcctgcc ccagggcgtc cgggtcgaga gcctgggcaa gctcctgcag
     6001 gacgagcccg agtggagtgc gagagcgctc ggcgggccgg cggagccgca cagcttcgcc
     6061 gatctgaacg cggccttcct gcaatccggc gcctggatcg agctcggcga cggggccgtg
     6121 ctggagcatc ccctgcagct cctcttcctc acggtcggtc agccggcggc ggtgatggcg
     6181 catccgcgga tcgtcgtccg gctcggcaag gaggcgcggc tgcacctgat cgagacccat
     6241 atcggcctcg ccccgggcca ctgcctgacc aatctcgtcg gccactacga gctcggcgag
     6301 aaggcgcgtc tgacccacga ccggctgcag ctcggcagtg ctgggaccag cttcgtcggg
     6361 ctcaaccggc aacgtctggc cgagcgcgcc gagctcaagc agaccctcgc cacgctcggc
     6421 ggggcgctgg tgcgcaacga gaccgacctg catctcgtcg gcagcttcgt cgaggcccag
     6481 ctcaacggtg tctacatgcc gatcggcgag gagcatgtcg acaacgtcat tcggattcat
     6541 cacgaagccc ccgacggcca ctcggaccag ttctacaaag gcgtgatgga cgaccgcgcg
     6601 catgccgcct tcgccggcaa gatcatcgtc caccaggacg cgcagcggac caacgccttc
     6661 cagcgcaacg acaatctcct gctctcggac gatgcggaga tctcgagcaa gccggagctc
     6721 gagatctatg ccgacgacgt caagtgcagc catggggcga cctgcggcga gctcgaccgg
     6781 acagcgctct tctatctgcg cagccgcggc ctcgaccggg cgcaggccga aagcatcctc
     6841 accttcgcct tcgccgccga ggtgatcgag cgcttcgtcg atcccgccgt ccgcaagctc
     6901 gcccgcgagt gcctggtggc gcgcacgccg ggcggcgccg gcctctcgga tctgctgtga
     6961 acgcgctcgc cacccgcccg gcgccacacg cggccctcga catcgaggcg gtgcgcgccg
     7021 agttcccgat cctcggcacg accatgcagg ggcagccgct cgtctatctc gacagtggtg
     7081 cgagcgccca gaagccgcgc cttgtgatcg acacgctgac cgaggtctac agcgccggct
     7141 acgccaacat ccaccgcggc gtctacgagc tgagccaggt cgcctcggcg cggcacgacg
     7201 aggcgaggcg caagctgcag cgcttcatcg gggccgccga ctggcgcgag atcgtcttca
     7261 cgcggaacgc caccgaggcg atcaacctcg tggcccaatg cttcacggca ccgctgctcg
     7321 gcccgggcga cgagatcctg gtgaccgaga tggagcacca cgccaacatc gtgccctggc
     7381 agatggtggc cgagccccgg ggtgcccggg tggtggcggc accggtgacg gacgcgggcg
     7441 agctcgacct cgacgccttc gaggggctga tcggcgagcg gacgcggatg atcgccgtgg
     7501 cctggatctc gaacgtgctg ggcacggtca acccggttgc ggagctcgtg gcgatggccc
     7561 gccggcatgg cgtgccgatc ctgatcgacg cggcccaggc ggtgcagcac acgagcgtcg
     7621 acgtggcggc gctcgacgcc gatttcctcg ccttctcggg gcacaagatg tacgggccct
     7681 cgggtgtcgg cgtgctctgg ggcaaggccg cacatctcga cgccatgccg ccctaccagg
     7741 gcggcggcga catgatcgag cgggtgagct tccagggcac cacctggaac gagaccccgt
     7801 tcaagttcga ggccggcacg cccgacatcg ccgggatcat cgcgctcggt gccgccgtcg
     7861 acttcatcga gggtctcggc atcgaggcga tcgccgcgca cgaggcggcg ctgctcgcct
     7921 acgccacgcg ggcgctgcag caggtgccgg gcctgcgcct gatcggcacg gcacgcgaca
     7981 aggccagtgt cctcgccttc gagctcgcgg gcgcccatcc gcaggatatc ggcatgctgc
     8041 tcgatctcga cggcatcgcc atccgcaccg ggcaccattg cgccatgccg ctgcacgagc
     8101 gcctggggat gagcgccagc gcccgcgcct cgatcggcgt ctacaacaac gaggcggata
     8161 tcgacgccct cgtccggagc ctcgggcggg tcgcggagat gttccagtga gagtggggga
     8221 agcgatggac gacaatggag tcggccgcgg cgcggaccgg ggcgccgacc aggggctcga
     8281 ccgggccgag gcgttcctcg gtgccgccgg cggcgaggag ccggctgcgg cctcgcccga
     8341 gcgcatggcc gaggtggacc gcctgggcga ggcgatcatc gcccagctca tgacgatctt
     8401 cgaccccgag atcccggtga acatctacga gctcgggctg atctacaaga tcgacgtcca
     8461 cgacggcaac acggtgacgg tcgacatgac cctgacctcg cccaactgcc cggtcgcgga
     8521 atcgctgccc atggaggtcc agcacaaggt cgagcaggtc gaggggatcg agggctgcga
     8581 ggtgcggatc gtctgggagc cgacctggca cccctcgatg atgaccgagg aggcacagct
     8641 cgagctcggc ctgatctact agcagttctg tgcgagcggg tgcttgtcgg gccgccgccg
     8701 ggcgcgcata accgcgccat gatcgacctg acgttccgcc agaagcatct gctcgggatc
     8761 gaggggctgc agccctggga gatcgccgat ctgctcgatt gcgccgatcg ctatgtcgat
     8821 ctcaaccggc gcccggcgaa gaagctgcag ctgctcgacg ggctgaccca gatcaacctc
     8881 ttcttcgaga gctcgacccg cacgctgacc tcgttcgagc tggccggcaa gcgactgggc
     8941 gccgacgtcg tcaacatggc ggtccagggc tcgtcgataa agaagggcga gacgctgatc
     9001 gacacggcga tgacgctcaa cgccatgcgc ccggacgtgc tggtcgtccg ccaccaggac
     9061 agcggtgcgg tgtcgctcct cgcccgccac gtcaactgct cggtgatcaa tgccggcgac
     9121 ggcagccacg agcatccgac ccaggcgctg ctggatgccc tcaccattcg ccgtcgcaag
     9181 gggcggctcg aggggctcac cgtcgccatc tgcggcgatg tccgccacag ccgggtggcg
     9241 cgctccaaca tcctcctgct gctggccatg ggggccagcg tccgactgat cgggccgccg
     9301 acgctgctgc cgagggcctt ctcgaccttc ggctgcgagc tcttcaccga catggcgaag
     9361 gggctccagg gctgcgacat cgtcatgatg ctgcgcctgc agaacgagcg gatgcagggc
     9421 agctacgtgc cttcggtgcg cgagtatttc catctctacg gcctgacccg ggagaagctg
     9481 gcgcacgccg cgcccgacgc gctcatcatg catcccgggc cgatgaaccg cggcgtcgag
     9541 atcgacgggg tcatcgccga cgacatcgac cggagcgcga tcctcgacca ggtcgagatg
     9601 ggcgtggcgg tgcgcatggc ctgcctcgac ctgctcaccc gcgactggcg agcggccgca
     9661 tgaggccgtt gctcttcacc gctgtccgcc tgctcgatcc ggcgaccggg ctcgatgcgc
     9721 cgggcgagct gctggtccag cagggcagga tcgccgcatt cgggccgagc gtcgaccgcc
     9781 cggacggggt ggagatcgtg gccggcgagg gggcttgcct ctgtccgggc ctcgtcgaca
     9841 tgcgggtgca tttcggcgag cccggcaccg agcacaagga gcggctcgcc tctggcatcc
     9901 gggccgcggc cgcgggcggc gtcacgtcgg tcgccgtcct gccgaacacg cggccgccga
     9961 tcgacgaccc ggcgatgctc gagttcgtcg cccgccgggc gcgccagctc aagtcggtca
    10021 aggtctaccc ctacgccgcg ctgaccaagg ggctcgaggg acgggagctc gcggagctgg
    10081 cgctgctcaa ggaagccggt gccgtggcct tcaccgatgc cgagcaggcg ctcgccgatg
    10141 cccgggtcat gcagcgggcg ctggcctatg cgagcgctct cgatctcctc gtcgtgcagc
    10201 accccgagga gccgagcctc gcatcggggg gcgtcgtcaa cgaaggggcc atcgccgttc
    10261 gcctcggcct gcggggcatc ccggcgatcg ccgaggtcat gatggtcgag cgcgacctgc
    10321 atctcgtgcg cgcgaccggt gcgcgctacc acgtcgccca catctcgacc agggcggcga
    10381 tcgacgccgt ccgccgggcc aaggcggagg gcctgcgcgt cacctgcgag accacgccgc
    10441 atcacctggc gctgaacgag ctcgaggtcg agggctaccg caccttcgcc aaggtcagcc
    10501 cgcccttgcg cgccgaggcg gaccgggagg ccgtggtcga ggggctggtc gacggcacca
    10561 tcgacgtcat cgccagcgac cactcgcccc aggaccagga cagcaagcgg ctgcccatgg
    10621 cccaggccga gttcggcgtg atcggggtcg agaccatgct gccggtgagc ctgcgcctgg
    10681 tgcacgaggg ccagatgagc ctgctcgatc tcctgcgcaa gctgacggtg gcgccggcag
    10741 acctgctcgg cctcgaggcc ggccggctgg cccagggtgc cgcggccgat ctcactctct
    10801 tcgatgccga gcgcccctgg aagatcaccg aggccgggct ctccagcctc gccaagaaca
    10861 ccgccttcga gggtcgtctg gtccagggcc gggtgctgct gaccatggtc gacggccggg
    10921 tggtgcaccg cttcacggca taggcgccgg ctcacggctc gctatccacc tcgtgagttg
    10981 tcgcgagcgg agcgatggcg gcataccgcc cgggcatgcg caacagccgg agggtccgac
    11041 catggctgcc accgatcgcc cgctcttcgg cgccgaccgt ctcctcacca tgctgctcgc
    11101 cggtgccgtc gccaccgccg ccttcgacct cttcgggcag agcctgagcc cgggcctcgg
    11161 cttcgccaat ctggcgccgg tgccgctcgc caacaacgtc atccaggtgc tgctcggggc
    11221 accctgggcg cccggcgccc acgcgctcca ctatctggcc gggctcgtcg gctatccgct
    11281 cggctggatg ctgatcgccc gcccgatcgc cctacggctg accccgacct ggccctggtg
    11341 gctgacctcg gcgatctacg gcgtcgggct ctggatcttc gccctcttca tcatggccca
    11401 cctggtcgcc ggccagccgg ccttcctggg cttcatcggc atcacctggg tggcgctggt
    11461 cggccacgtc gtctacgcca tcgcggccgc tgccgtcgtc cagtggcgcg agccgggctg
    11521 aaccgcaccg ccgcgtcact cgacgcactg gttccggtct cgggggtcgg atagactccg
    11581 gtcgacgaac gttgcactga cgaggatggg ctgaaccatg a
//
LOCUS       DAOTFI010000153        11591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6620_length_11591_cov_7.749827, whole genome shotgun sequence.
ACCESSION   DAOTFI010000153 DAOTFI010000000
VERSION     DAOTFI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6620_length_11591_cov_7.749827"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(967..3420)
                     /locus_tag="P5558_15440"
     CDS             complement(967..3420)
                     /locus_tag="P5558_15440"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRY25761.1"
                     /translation="MNEAAFALPPRSIDTSGIRKAILGKLIYAVGKDPEHATVHDWFF
                     ATALATRDRMVDAWMDSTRETYARDRRRVYYLSLEFLIGRLLADTLNNLHLMTPTREA
                     LAVLGVDADAVFRVEPDAALGNGGLGRLAACFMESMASLGIAGFGYGIRYEHGLFKQG
                     FDKGWQVERPEDWLAFGNPWEFERPEAVYPVRFGGQVRDVTDADGRRRYVWEGGQRVL
                     AVAYDTMIAGWGGGHVNTLRLWSGQSGNLIDLEAFNRGDYKEAVGEQIAAESISRVLY
                     PNDATQAGQELRLKQEYFFTAASLMDILRRHLSYHDTLDNLADHVAIQLNDTHPAIAV
                     PELLRLLVDEHDMDWHRAFAIVQDTIHYTNHTLLPEALERWPVQLMEQMLPRHMQLIY
                     EINAHVLGTLRHLPGNDDPFLADVSLIEEGYGRAVRMGHLAFVGARKVNGVSALHTEL
                     MKQTVFRSLHGHFPDKITNKTNGITPRRWLMGCNPGLAALYDDCMGAGWRDDIGRLEA
                     LEPRLSDAGFLDRFMAVKRRNKEALATLIGDQHGLAVDPAALFDVQIKRIHEYKRQLL
                     NILEAIALYDAIREAPERDWPKRIKLFAGKAAPSYNRAKLIIKLINDVAEVVNSDPLV
                     AGRLQVHFMPNYNVSLAERMIPAADLSEQISTAGMEASGTGNMKFALNGALTAGTLDG
                     ANVEMREHVGAENFFIFGLTAAEVIHARTLRDASRAAIAASPELARALELIERGRFSP
                     GDPGRFRPIVDDLRNFDHFMVTIDFAAYWQAQRAIDTVFADPARWSPKAAANAIRMAW
                     FSSDRTIREYAEEIWRCPV"
     gene            complement(3572..4852)
                     /locus_tag="P5558_15445"
     CDS             complement(3572..4852)
                     /locus_tag="P5558_15445"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY25762.1"
                     /translation="MGTGRSAAGPLGTWLALQLPRLEYAVFAFLLALGAGALGFIDNR
                     LYAVGTDFPTQFSTFTILSWPAVYLLFAAFMLHRPEVLRQGIAAAPWVFAFPLVAALS
                     VAWSMDRAVTAGEAFRLVMTVLIGVYLGARLSVVGLARLTALVLGVAVGASVLLHLAG
                     VDFATMINGAARGVFYHKNTLGNAAVVLIAAATALLVGRPTDPLGLLGLVAGLAALPF
                     ADSATAIVTAGIVLAGTGVLVLRNRPLILALWVGFLLIALALVVGFFLALGTSPVELL
                     FGALDKDPTLTGRSYLWETGFLQLEQRPVLGTGFAAFWTAAVDWRTLRVLDLLGQVGH
                     FHNTLLEIAVELGLVGVVAALATLGLYTRTAWRHLRSTRDRRAVFAWLFLFVLVATGL
                     TEVYGFVKHSTATLLLVALAVAMGKELGAFARRR"
     gene            complement(4864..6327)
                     /locus_tag="P5558_15450"
     CDS             complement(4864..6327)
                     /locus_tag="P5558_15450"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRY25763.1"
                     /translation="MAEGFGRRAVKGSLWATFDGWATEALSLVVFLVLARLLGPGEFG
                     LVAIALGFTAVAAHLTAYTTGEVLIQKQDLTDADCDAVFWVTVLAAGLLALVLALAAP
                     AIGRLFDAAELASVIRWLCVVLVLQAFTTVPLALLTRELRFDVTARRSLIMLLGGAIT
                     GIAMAVQGYGVWALVGQHLAGASLNTVLLLGATGWRPGLRAGWPQVLAIRRFVASSLG
                     NSALQMVDERAPQLLVGFLLGPAAAGLMNVALRLVQMMQRLFAVPINQIAMPAFARLR
                     DDPAALDRFRDVALSLGLAVSLPAAAGAAAIAPELIGLGLGEAWLPMVPVFQILCVGV
                     AIWPVILQARSALYGLGRPERLLRVGLLDGVAGVIVILATAPFGLVAVALGLSLRILV
                     WRWPLTARQVRLELGTGLVHELRLLLPPLLASLLVLLVFVALRLALEPVLGTWPAIVL
                     AGLVAGLAYVVITAVLQRSHLRVAWALWRGVPVAAPA"
     gene            complement(6320..7246)
                     /locus_tag="P5558_15455"
     CDS             complement(6320..7246)
                     /locus_tag="P5558_15455"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY25764.1"
                     /translation="MDYTIAIVTRGRPAGLARVLEALGHCRLPSPRSTVLVVDNDPAR
                     TAEPVVAALAAGHPLALDYLHEPRPGVVFGRNAALDQARGTRLAFVDDDEEPEPGWLV
                     ALAAAMDAADAQAVSGPVRPVFAKPMPAWFAEAFALCYVRPLADGSLGELMTGNLLLD
                     RPFLARHGLRFPEALATHGAEDTALSALLLEAGGRLAWAADAVVLEHIPAERLGLAWL
                     LARWYRYGMSDVRIRLLERASPAVRLQALGRGALRVGGGGLLFLLALPGLVLARPGPV
                     VRRCYTIMRGLGMIAAGLGFASEPYAVRASRG"
     gene            7480..9843
                     /locus_tag="P5558_15460"
     CDS             7480..9843
                     /locus_tag="P5558_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRY25765.1"
                     /translation="MAWGSDSGRLPALAGGSEPAGSADGRLTSGFAAIVNAIRRYWLS
                     TLILIVLGTALGVAVHRLLPTEYEATATVLIGGPQARQPELDSLVVSAVLNQDTLTNE
                     VEILQSRALALRVIERLQLHRLDAFNPPGPEPGSGSSRSGIAQWLRDQVGALRAWAGQ
                     LPLGNDAVDDADYGRSEMEDVIDRFLEDVTIAPRGRSQVIDVTFRTEAPGLAALVANT
                     LVEEYLVDRTDNQTAAARLAGSWLRERLGELRDELQRRETAVEDYRREAGLLQGVTAS
                     LTSERLSGLNSQLLEARAALAQASGRYDEAMAAREAGRGAAMAEVLQSETIRDLRAQE
                     SEIASEVAQFENVYGPQHPRMMDLRQHLAQVRRGIAGEVDRILASLASATETYRQRVE
                     LLEVEVAEVRAKVVQEDAAMARLRVLEREAEATRELYQDTLRRAEAADPTPATTPSDG
                     RMLSPAAPPTVSTKPRLALMGGLGFVFGCGLALVTAVGRELLDRRFRTVGQAEAQLGL
                     RVIGVVPSLEGLPGQPPEFEPEDTVVIYPGGAFAEAVRTVTNQLRSLPRRDATGTPGG
                     SAYLPSDPDKDPPGALILAVTSSVQGEGKTMLAVSLARSAALSDQRVLLVDADMRLGR
                     VHQALAIPATPGLADYLAEPADKEIASLWQSDPSTRLKVLTCGKQGYKAGVLLQSGHL
                     RRAMQSLRRQFDLIIIDTPPVLPVADFGTLVDVADAALFIVDWSRMRPDVIEAGAARI
                     RATRAGLELGVVINNVDVRKKAADAFPELEIYSSRYRNDSRYHYHSS"
     gene            complement(9865..10833)
                     /locus_tag="P5558_15465"
     CDS             complement(9865..10833)
                     /locus_tag="P5558_15465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY25766.1"
                     /translation="MSASVPAVSVVVPVFNSARCLPAAIASVQAQTFTDWEIVLVDDA
                     STDTSPALIEALIASDPRIRAARLPANAGPAAARNRGLELARGNWIALLDADDAYFAD
                     RLAVLTALGASTGADLVSDNLLLADPVDDRERGLAWPAASLAEAPWIDAASYLASNLF
                     HRREAGYGFMKPLVARAFIDRHGLRYDPALRLGEDYEFYWTCLQHGARWRVLETAHYR
                     YHLTPGSATRRITPEQIQIMRDRAEAALRAAGDPRLLPLLRRRLRQLRGFHQHHAAMR
                     DLKAGRFAAGFRRLVGEPAVLPFVVDALRTGLPKRLGLRGRHDHGF"
     gene            complement(10851..>11591)
                     /locus_tag="P5558_15470"
     CDS             complement(10851..>11591)
                     /locus_tag="P5558_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRY25767.1"
                     /translation="NLVLAPQLFGREPLLDVYWTLQVELVFYVACAGLAVLGLIHLRH
                     LAGWLAMLGLAGALAAAALKHFLLIDLPVGLALWLSLMFWGFAAREADISGDRLVARQ
                     VQLLFWAFLLALGPICYLGYHRLPSVYGTWQSYFWSYALAVVIFWAFAIRWPWSAGWL
                     TRVGRISYSIYLLHVLCIIVLGAWLGPWLAGRVPGLVFIGLVGLASVAVSAPTFAWLE
                     QPAINLGREVNRRWRAAPDGRLRQRPLL"
BASE COUNT         1960 a         4034 c         4179 g         1418 t
ORIGIN      
        1 acgagcaagg aggaagcgag caggcgcatg gccgtgtctc cactggcacg aggattcccc
       61 atcgatcgaa ctggcgcccc gatcgctgaa atccagcatc tgccggtccg aaaggctcgg
      121 gtgccgaaaa aacgcaggtg acagtcgcga tcgcgcgcgc aagggcgacg tgccgaaagc
      181 cggagagccg ggcgagagcg aggcagaccg ttcgggcgcc ggcgaagcgg gcggagccgc
      241 gctgccagcg gcggccggac ccgagacccg gccgggtggt ggccgcgcct agcagattcg
      301 aacgcgccga gcacagggcg ccggcgcctt gccgaacgcg gctcggatgg gcacgcaccc
      361 ggcttgccgg gtcgcgctcc cgagccgggc ccgccatatg gccaagggcc gacccctcaa
      421 cgacccgatg ccggtctcgc gagcgcgcct gcgcatggcc aacgaagacc gccgcgcgcg
      481 cttcgacggc gaagcaggcg ccagggccgc agccgcggcc ggactgcgcg ctcggcccga
      541 agggcatccc tgccggtgat ccctgaaagc cgcgggggca gaacgccgag ctcctcgtcc
      601 cgtggaacgc ctgcaagccg ccgcggaaac cggcaccgat gaatggcggc agcacagggc
      661 gctacgcggc gctgcggccg accgaagcac attcggcggg cagagcgatg agctggtcgt
      721 cggcagatca cgcccgggcg ctcgagcatc ctcatggcga ccgcatccgt cgccaggcgg
      781 cgggcgatcg gtgcttgcgc aagtcaacga cgctgcacgc cgattgcccc gttcagggta
      841 atgggcgccc ctcgtccacc atcactgcca aggcccccct atccgcgcaa gatcgagcca
      901 tgcccggctg ccgcccgccc tctgcccctc tgcccctctg cccctctgcc gctcgccctc
      961 tgcccctcaa acgggacaac gccagatctc ctcggcgtat tcccggatgg tgcggtcgga
     1021 ggagaaccag gccatgcgga tcgcgttggc cgccgccttg gggctccagc gcgccgggtc
     1081 ggcgaatacc gtgtcgatcg cccgctgcgc ctgccagtag gcggcgaaat cgatcgtcac
     1141 catgaaatgg tcgaaattgc gcaaatcgtc gacgatcggc cggaagcggc cgggatcgcc
     1201 cggcgagaac cggccgcgct cgatcagctc gagcgcgcgc gccagctcgg gcgacgcggc
     1261 gatggcggca cgcgaggcgt cccggagggt gcgcgcgtgg atcacctcgg cggcggtcag
     1321 gccgaagatg aagaagttct cggcccccac atgctcgcgc atctcgacat tggcgccatc
     1381 gagcgtgccg gcggtcaagg ctccgttcag cgcgaacttc atgttgccgg tgcccgaggc
     1441 ctccatgccg gcggtcgaga tctgctcgga gagatcggcc gccgggatca tccgctcggc
     1501 caggctgaca ttgtagttgg gcatgaagtg cacctgcagc cggccggcga ccaggggatc
     1561 gctgttgacc acctcggcca cgtcgttgat cagcttgatg atcagcttgg cgcggttgta
     1621 ggacggcgcc gccttgccgg cgaagagctt gatccgcttc ggccagtcgc gctccggcgc
     1681 ctcgcggatg gcgtcgtaga gggcgatcgc ctcgaggatg ttgagaagct ggcgcttgta
     1741 ctcgtggatg cgcttgatct gcacgtcgaa gagggcggcg gggtcgaccg cgaggccgtg
     1801 ctggtcgccg atcagcgtcg ccagcgcctc cttgttgcgc cgcttcaccg ccatgaaccg
     1861 gtcgaggaag ccggcatccg aaagccgggg ctcgagcgcc tcgaggcgac cgatatcgtc
     1921 gcgccagccg gcacccatgc agtcatcgta aagggcggcg aggccggggt tgcagcccat
     1981 cagccagcgc ctgggcgtga tgccgttggt cttgttggtg atcttgtcgg ggaaatggcc
     2041 gtgcagcgac ctgaacaccg tctgcttcat cagctcggtg tgcagcgccg acacgccatt
     2101 gaccttgcgg gcaccgacga aggccagatg gcccatgcgc acggcccggc catacccctc
     2161 ctcgatcagc gagacgtcgg cgaggaacgg gtcgtcattg cccggcagat ggcgaagcgt
     2221 gccgagcaca tgggcgttga tctcgtagat gagctgcatg tgcctcggca gcatctgctc
     2281 catgagctgc accggccagc gctccagcgc ctcgggcagg agcgtgtggt tggtgtagtg
     2341 gatggtgtcc tggacgatgg cgaaggcgcg gtgccagtcc atgtcgtgct cgtcgaccag
     2401 gagccgcagc agctccggca cggcgatcgc cggatgcgtg tcgttgagct ggatggcgac
     2461 gtggtcggcg agattgtcga gcgtgtcgtg ataggagaga tggcggcgga ggatgtccat
     2521 caggctcgcc gccgtgaaga aatactcctg cttgaggcga agctcctggc cggcctgggt
     2581 ggcgtcgttg gggtagagca cccggctgat gctctcggcg gcgatctgct cgccgacggc
     2641 ctccttgtag tcgccgcgat tgaaggcctc gaggtcgatc aggttgccgg actggccgga
     2701 ccagaggcgc agcgtgttga cgtgcccgcc cccccagccg gcgatcatcg tgtcgtaggc
     2761 gacggccagg acgcgctgcc cgccctccca gacgtagcgg cgccggccat cggcgtcggt
     2821 cacgtcgcgc acctggccac cgaagcgcac cgggtagacc gcctccggcc gctcgaactc
     2881 ccagggattg ccgaaggcca gccagtcctc cggccgctcg acctgccagc ccttgtcgaa
     2941 gccctgcttg aagaggccgt gctcgtagcg aatgccgtag ccgaagccgg cgatgccgag
     3001 gctcgccatg ctttccatga agcaggcggc gagccggccg aggccgccat tgccgagcgc
     3061 tgcgtccggc tcgacccgga agaccgcatc tgcatcgacg ccgagcaccg ccagcgcctc
     3121 gcgcgtcggc gtcatcaggt gcaggttgtt cagcgtgtcg gccaggagcc ggccgatcag
     3181 gaactcgagg ctgaggtagt agacccgccg ccggtcgcgc gcataggtct cgcgcgtgct
     3241 gtccatccag gcgtcgacca tccggtcgcg cgtggcgagg gcggtggcga agaaccagtc
     3301 gtggacggtc gcgtgctcgg gatccttgcc aacggcatag atcagcttgc cgaggatcgc
     3361 cttccggatg ccggaggtgt cgatcgagcg gggcggcagg gcgaaggcgg cttcgttcat
     3421 cgatgcacgg ttcccagatg gtcgaagacg gcgataaacg cccatcgccg cgacacggca
     3481 agcgggaatg ctccaccacc cccgggttcc gcccgcgatc aggcacctcg gccagtgatg
     3541 cccggtctag acgaaggaga tgacggatcg gttaacggcg tcgagcaaag gccccgagct
     3601 ccttccccat cgccaccgcc agggcgacga gcagcagggt ggcggtgctg tgcttgacga
     3661 agccgtagac ctcggtgagg cccgtcgcga ccaggacgaa gaggaagagc caggcgaaga
     3721 cggcccgccg gtccctggtg ctgcgcagat gccgccaggc tgtccgcgtg tagagtccga
     3781 gcgtcgcgag cgccgccacg acgccgacga gaccgagctc gaccgcgatc tcgagcaggg
     3841 tgttgtggaa atgccccacc tggccgagca ggtcgagcac gcgcagcgtc cgccaatcga
     3901 ccgccgccgt ccagaacgcg gcgaagccgg tgcccagcac cggccgctgc tcgagctgca
     3961 ggaacccggt ctcccagaga taggagcgcc cggtgagcgt cggatccttg tcgagcgcgc
     4021 cgaagagcag ctcgaccggc gaggtgccga gtgccaggaa aaacccgacg accagggcca
     4081 gggcgatcag gaggaacccg acccagagcg ccaggatcaa agggcggttg cgcagcacga
     4141 gcacgcccgt gccggcgagg acgatgccgg cggtgacgat cgccgtggcg gaatcggcga
     4201 acggcagggc ggcgagaccg gcgacgaggc ccaggagccc gagcggatcg gtcggccggc
     4261 cgacgaggag tgcggtggcg gcggcgatca gcacgaccgc ggcgttgccc agcgtgttct
     4321 tgtggtagaa gacaccgcgc gcggcgccat tgatcatcgt cgcgaaatcg acaccggcca
     4381 gatgcagcag caccgaggca ccgaccgcga cgccgagcac cagggcggtc agccgggcga
     4441 gcccgacgac ggacagccgc gcacccagat agacgccgat cagcaccgtc atgacgagcc
     4501 ggaaggcctc gccggcggtc accgcccggt ccatcgacca ggcgaccgag agtgccgcca
     4561 ccaggggaaa ggcgaagacc cagggcgccg ccgcgatccc ctggcgcagc acctccggcc
     4621 gatgcagcat gaacgccgcg aagagcagat agacggccgg ccagctgagg atggtgaacg
     4681 tgctgaattg cgtcggaaaa tccgtgccga cggcatagag gcgattgtcg atgaagccga
     4741 gcgcccccgc cccgagcgcc aggaggaagg cgaagacggc gtattcgagg cgcggcagct
     4801 gcagggcgag ccaggtgccg aggggaccgg cagccgaccg gccggtgccc atccccgcgc
     4861 tcctcatgcc ggggccgcca ccggcacgcc gcgccagagc gcccaggcca cgcgcagatg
     4921 cgaccgctgc agcacggcgg tgatcacgac ataggcgagc ccggcgacaa gaccggccag
     4981 cacgatggcc ggccaggtgc cgagcaccgg ctcgagcgcc agccggagcg cgacgaagac
     5041 cagaagcacg aggaggctgg ccaggagtgg cggcaggagg agccggagct cgtgcacgag
     5101 gcccgtgccg agctccagcc gcacctgccg cgccgtcagc ggccaccgcc agacgaggat
     5161 gcgcaggctc aggcccagcg cgaccgcgac gagcccgaac ggtgcggtgg cgaggatcac
     5221 gatcacgccg gcgacgccgt cgagcagacc gacccgcagc agccgctcgg gccggccgag
     5281 gccgtagagc gccgagcgcg cctgcaggat caccggccag atggcgacgc cgacgcagag
     5341 gatctggaag accggcacca tcggcagcca tgcctcaccc aggccgaggc cgatgagctc
     5401 cggggcgatc gccgcggccc ccgccgcggc cggcaggctg accgcgagcc cgagcgacag
     5461 ggcgacgtcg cgaaaccggt cgagggccgc cggatcgtcg cgcagccggg cgaaggccgg
     5521 catggcgatc tggttgatcg gcaccgcgaa cagccgctgc atcatctgca cgaggcgaag
     5581 cgccacgttc atcaaccccg ccgcggcggg ccccaggagg aagccgacca gaagctgcgg
     5641 cgcccgctcg tcgaccatct gcaaggcgct gttgccgagg ctgctggcga cgaagcgacg
     5701 gatcgccagg acctgcggcc agccggcgcg caacccgggg cgccagcccg tcgcacccag
     5761 caggagaacg gtgttgagcg aggcgccggc gagatgctgg ccgaccagag cccagacgcc
     5821 atagccctgc accgccatgg cgatgccggt gatcgcaccg cccaggagca tgatgagcga
     5881 gcgcctggcg gtgacgtcga agcgcagctc gcgggtgagc agcgccaggg gcacggtggt
     5941 gaaggcctgc agcacgagga cgacgcagag ccagcggatc accgatgcca gctcggcggc
     6001 atcgaacagc cggccgatcg ccggcgccgc cagagccagc accagagcga gcagtccggc
     6061 cgccagaacg gtcacccaga agacggcgtc gcaatcggca tcggtgagat cctgcttctg
     6121 gatcagcacc tcgccggtcg tgtaggcggt gagatgggcc gcgaccgcgg tgaagccgag
     6181 cgcaatggca acgaggccga actcgcccgg gccgagcagc cgggcgagca ccaggaagac
     6241 gacgaggctg agcgcctcgg tcgcccagcc gtcgaaggtg gcccagaggc tccccttgac
     6301 cgcgcgccga ccgaacccct cagccacggc tcgcccggac cgcgtagggc tcgctcgcga
     6361 agccgagacc ggccgcgatc atcccgaggc cgcgcatgat cgtgtagcag cgccgcacca
     6421 cggggcccgg tcgtgccagg accagccccg gcaaggcgag gaggaaaagc agcccgccgc
     6481 caccgacccg aagcgcaccg cgcccgagag cctgcagccg aaccgccggg gaagcccgct
     6541 cgagcagacg aatccgcaca tccgacatgc cgtagcggta ccaccgggcg agcagccagg
     6601 cgagcccgag acgctcggcg gggatatgct ccaggaccac cgcgtccgcc gcccaggcga
     6661 gcctgccgcc ggcttcgagc agcagcgccg agagtgccgt gtcctccgca ccatgcgtcg
     6721 ccagcgcctc ggggaagcgc aggccgtgcc gggcgaggaa cgggcggtcg agcaggaggt
     6781 tgccggtcat cagctcgccg agcgagccgt cggccagtgg ccgaacatag cacagggcaa
     6841 aggcctcggc gaaccaggcc ggcatcggct tcgcgaaaac cggccggacc gggccgctga
     6901 ccgcctgggc atcggcggcg tccatggccg ccgccagggc cacgagccag cccggctcgg
     6961 gctcctcgtc gtcgtcgacg aaggcgaggc gggtgccccg ggcctggtcg agcgccgcgt
     7021 tgcggccgaa gacgacgccc ggccggggct cgtgcaggta gtcgagggcc aaaggatgac
     7081 cggcggcgag ggcagccacc accggctcgg ccgtgcgcgc ggggtcgttg tcgacgacga
     7141 gcacggtgct tcgtggcgac ggcaggcgac aatgcccgag cgcctcgagc acccgcgcca
     7201 gaccggccgg ccggccgcgg gtgacaatgg cgatggtgta gtccattctg acacctgaca
     7261 atcgctttgg ttgaatctga cggctaccca atcaatcgat gccgtttgtc tcgaaaagca
     7321 cggtggaaac ctgccgcaac tcttcccgcg ggcggcccag ctttcgcttg aaggccccgg
     7381 agcggacagg gtaacttacg ttaggcgatc cactctccgg tgcaagccgg ggtggggtgc
     7441 cgcaggtgat cgaaagacga acgggagagg atgcggcact tggcttgggg atccgatagc
     7501 gggcggctgc cggcgctcgc cggcgggtcc gagcccgccg gatcggcgga tgggcggctg
     7561 acgtcgggtt ttgccgccat cgtcaacgca attcgcaggt actggctctc gacgctgatc
     7621 ctgatcgtgc tcggcacggc gctcggtgtg gccgtgcatc gcctgctgcc gaccgagtac
     7681 gaggcgacgg cgaccgtgct gatcggcggc ccgcaggccc gccagccgga gctcgacagc
     7741 ctcgtcgtca gcgccgtgct caaccaggac acgctgacca acgaggtcga gatcctgcag
     7801 tcgcgggccc tggcgctcag ggtcatcgag cggctgcagc tgcatcggct cgacgcgttc
     7861 aacccgcccg ggcccgagcc ggggtccggc agctcccgga gcggcatcgc gcaatggctg
     7921 cgcgaccagg tcggcgcgct cagggcctgg gccggccagc tgccgctcgg caacgacgcg
     7981 gtggacgacg ccgattacgg ccgcagcgag atggaggacg tgatcgaccg cttcctcgag
     8041 gacgtcacca tcgccccgcg cggccgctcg caggtcatcg acgtcacctt caggaccgaa
     8101 gcacccggcc tcgcggccct ggtcgcgaac accctggtcg aggaatatct ggtcgatcgc
     8161 accgacaacc agacggccgc ggcaaggctc gccggaagct ggctgcgcga gcgcctgggc
     8221 gagctcaggg acgagctcca gcggcgcgag accgcggtcg aggactaccg ccgcgaggcg
     8281 ggcctcctgc agggcgtgac cgcatccctg acctccgagc ggttgtcggg gctgaacagc
     8341 cagctcctcg aggcaagggc ggccctcgcc caggcgagcg gccgctacga cgaggcgatg
     8401 gccgcaaggg aagccggccg cggcgccgcc atggccgagg tgctgcaatc cgagacgatc
     8461 cgcgacctcc gggcgcagga gagcgagatc gccagcgagg tcgcccagtt cgagaacgtc
     8521 tacggccccc agcatccgcg catgatggac cttcgccagc atctcgccca ggtccggcgg
     8581 ggcatcgccg gcgaggtcga ccgcatcctc gccagcctcg ccagtgccac cgagacctac
     8641 cgccagcggg tcgagctgct cgaggtcgag gtggccgagg tgcgtgccaa ggtggtccag
     8701 gaggatgcgg ccatggcgcg gctccgcgtg ctcgagcgcg aggccgaggc gacccgcgag
     8761 ctctaccagg acacgctgcg ccgggccgag gcagcggacc cgacaccggc caccacgccc
     8821 tccgacggcc ggatgctgtc gcccgccgcc ccgccgacgg tctcgaccaa gccgcgcctc
     8881 gccctgatgg gtggcctcgg cttcgttttc ggctgcggcc tcgccctcgt cacggcggtc
     8941 ggccgggagc tgctcgaccg gcgcttccgc acggtcggcc aggccgaggc gcagctcggg
     9001 ctacgggtga tcggcgtggt gccgagcctg gagggcctgc ccggccagcc gccggagttc
     9061 gagcccgagg acaccgtggt gatctatccg ggcggcgcct tcgccgaggc ggtccggacc
     9121 gtgaccaacc agctgcgcag cctgccccgc cgcgatgcta ccggcacgcc gggcggctcc
     9181 gcctacctgc cgagcgatcc ggacaaggat ccgccgggag ccctcatcct cgccgtcacc
     9241 tcctcggtgc agggcgaagg caagaccatg ctcgccgtct cgctcgcccg ctcggcagcg
     9301 ctcagcgacc agcgggtgct gctcgtggat gccgacatgc ggctcggccg ggtgcaccag
     9361 gcgctcgcca tcccggcgac gccgggtctc gccgactatc tcgccgagcc ggcggacaag
     9421 gagatcgcga gcctctggca gagcgatccg tcgacccgcc tcaaggtgct gacctgcggc
     9481 aagcagggct acaaggcggg cgtgctcctg cagtcggggc acctgcgccg cgcgatgcag
     9541 agcttgcgcc ggcagttcga cctgatcatc atcgacaccc cgccggtcct gccggtggcc
     9601 gatttcggca cgctggtcga cgtcgccgac gccgcgctct tcatcgtcga ctggtcgcgc
     9661 atgcgacccg atgtgatcga ggccggcgcc gcccggatca gggcgacccg ggccgggctc
     9721 gagctcggcg tcgtcatcaa caatgtcgat gtccgcaaga aggccgccga cgccttcccc
     9781 gagctcgaga tctacagcag ccgctatcgc aacgactccc gctaccacta ccacagctcg
     9841 tgacagccgg ccgccggccc ggcctcagaa gccgtggtcg tggcggccgc gcaggccgag
     9901 ccgcttgggc aggccggtgc gcagcgcgtc gacgacgaag ggcaggacgg ccggctcgcc
     9961 gaccagccgg cggaacccgg cggcgaagcg cccggccttc agatcgcgca tcgccgcgtg
    10021 atgctggtgg aagccccgaa gctggcgcaa ccggcggcgc agcagcggca ggaggcgcgg
    10081 gtcgccggcc gcgcgcagcg ccgcctccgc ccggtcacgc atgatctgga tctgctccgg
    10141 cgtgatccgc cgcgtggccg agcccggggt caggtgatag cggtaatgcg ccgtctccag
    10201 gacccgccag cgggcgccgt gctgcaggca ggtccagtag aactcgtaat cctcgccgag
    10261 ccgcagcgcc gggtcgtagc gcagcccgtg ccggtcgatg aacgcgcggg cgacgagcgg
    10321 cttcatgaag ccgtacccgg cctcgcgccg atggaagaga ttgctcgcga gatagctcgc
    10381 ggcatcgatc cagggcgcct cggcgaggct cgccgccggc caggcgaggc cgcgctcgcg
    10441 gtcgtccacc ggatcggcga gcaggagatt gtccgagacc agatcggcac ccgtgctggc
    10501 accgagagcc gtcagcaccg ccaggcggtc ggcgaaatag gcgtcgtcgg cgtcgagcag
    10561 cgctatccag ttgccgcggg cgagctccag cccgcggttg cgggcggcgg ccggcccggc
    10621 attcgccggc agacgggccg cccggatccg cgggtcgctg gcgatcagcg cctcgatcag
    10681 cgccgggctc gtgtcggtcg aggcatcgtc gaccaggacg atctcccagt cggtgaaggt
    10741 ctgcgcctgg accgaggcga tcgccgccgg caggcagcgc gccgaattga agacgggcac
    10801 gacaacgctg acggccggca cgcttgcgga cacgagctcg accacctcgg tcagaggagc
    10861 ggccgctgcc gcaggcggcc gtccggggcg gcgcgccagc gccggttgac ctcgcggccg
    10921 agattgatcg ccggctgctc cagccaggcg aaggtcggcg ccgagacggc gacgctggcg
    10981 agaccgacga gcccgatgaa gaccagcccg ggcacccgac cggcgagcca ggggccgagc
    11041 caggcgccga gcacgatgat gcacagcacg tgcaggaggt agatcgaata gctgatccgc
    11101 ccgacccggg tgagccagcc ggcgctccag ggccagcgaa tggcgaacgc ccagaagatc
    11161 accacggcca gcgcgtagga ccagaaatag ctctgccagg tgccatagac cgagggcagc
    11221 cggtggtagc cgagatagca gatcgggccg agcgccagca ggaaggccca gaagaggagc
    11281 tgcacctggc gggcgacgag gcggtcgccg ctgatgtcgg cctcgcgcgc ggcgaagccc
    11341 cagaacatca ggctgagcca caaggcgagg ccgaccggca ggtcgatcag gaggaaatgc
    11401 ttgagcgccg ccgcggccag ggcgccggcg aggccgagca tcgccagcca gccggcgaga
    11461 tgccgcaggt ggatcaggcc gagcacggcg aggccggcgc aggcgacata gaagacgagc
    11521 tcgacctgca gcgtccagta gacatcgagc aggggctcgc gaccgaagag ctgcggcgcc
    11581 agcaccaggt t
//
LOCUS       DAOTFI010000154        11578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6628_length_11578_cov_6.748503, whole genome shotgun sequence.
ACCESSION   DAOTFI010000154 DAOTFI010000000
VERSION     DAOTFI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_11578_cov_6.748503"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="P5558_15475"
     CDS             complement(<1..940)
                     /locus_tag="P5558_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY25768.1"
                     /translation="MSLLAVDDLSVSFRAGELEIEAVRKVSFTIERGETLALVGESGS
                     GKSVTALSILQLLPYPKAWHPSGSVRLDGEELLGKSDAVLRQVRGNRASMIFQEPMTS
                     LNPLHTVEQQIGEVLELHRGLMGDAIRARVLELLELVRIDEAETRLRSYPHQLSGGQR
                     QRVMIAMALANEPDLLIADEPTTALDVTVQAQILALIAELQERLGMAVLLITHDLTIV
                     RKVAHRVAVMTEGEIVESGPTEAIFTGAQHSYTRHLLAAEPKGAPPVADGSAPVILAG
                     QDIKVHFPIQRGLLRRTVGHVKAVDGVTLEVRAGQTV"
     gene            complement(937..2037)
                     /locus_tag="P5558_15480"
     CDS             complement(937..2037)
                     /locus_tag="P5558_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25769.1"
                     /translation="MRHEPRLLGFRLRLNPINERRYWNFRNNRRGYWSLHIFVLVFVL
                     SLFAEFIANERPLLVSYDGQLYFPVLVGYPETEFGGFFPTDTDYHDPFVRELIEENGW
                     ILWPPIPYSYNTIVRDLDAPAPSPPTWQNLLGTDDQARDVLARVIYGFRISVLFGLTL
                     TVLSSIVGIAAGALQGYFGGWVDLGFQRFMEIWSGLPTLYLLIILASIVTPNFWWLLG
                     LMLLFSWMGLVDVVRAEFLRARNLDFVRAARALGVGNATIMFRHILPNAMVASLTFMP
                     FILNGSITTLTSLDFLGFGLPPGSPSLGELLAQGKNNLQAPWLGISGFMVLAIMLSLL
                     IFIFEAVRDAFDPRKTFAALAVDAAPHARGHA"
     gene            complement(2037..3128)
                     /locus_tag="P5558_15485"
     CDS             complement(2037..3128)
                     /locus_tag="P5558_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HRY25770.1"
                     /translation="MLAYVVRRLLLIIPTLFGIMVVNFVVIQAAPGGPVEQMIAEISG
                     TAVASTARLTGGGGDGPRRPAQEPGSQYRGAQGLDPRFVAEIERMYGFDKPAGERFFL
                     MLGNYIRLDFGKSFFSDKSVIGLIGEKLPVSISLGLWTTLLTYLISIPLGIRKAVRDG
                     SSFDVWTSGVVIVGSAIPSFLFAILLIVLFAGGSYLNWFPLRGLVSDNWSDLSWPMRI
                     VDYFWHMTLPLLSLMIGAFAGLTMLTKNSFLDEINKQYVVTARAKGLTERRVLYHHVF
                     RNSMLIVIAGFPGAFIGILFTGALLTEIIFSLDGLGLLGYEAALRRDYPVMFGSLFIF
                     TLLGLVVKLISDLTYVLVDPRIDFEGRAG"
     gene            complement(3147..5003)
                     /locus_tag="P5558_15490"
     CDS             complement(3147..5003)
                     /locus_tag="P5558_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25771.1"
                     /translation="MTRTAMIALAATLAVASVAGAAETATPSHGVAMHGDLAYPPDFT
                     HFAYADPAAPKGGEIVFSAIGSFDSLNPFILRGQAAAGAANVFETLTERSRDEPFAAY
                     GLLAETIEMPEDRSWVAFTLRPEARWHDGQPVTVEDVIFSLETIKTRGHPLYRAYYAN
                     VTGAEPAGERRVLFHFDGELNRELPLIIGELPILPKHFWEGRDFEATTLEPLLGSGPY
                     RVARVDPGRSLTLERVADYWGQDLPVNKGRYNFDRMRYEYFRDGNVALEAFKAGQYDI
                     RIENIARLWATGYTGPAIDAGLIVKEEIQDETGDGMQGFVFNTRREIFSDPRVRAALA
                     YAFDFEWSNQTLFYGQYARTKSYFSNTDLAARELPDERELRILEPFRAELPPEVFTTV
                     YEPPVTDGSGELRDNLRTALELLREAGWEIDRSTRKLVNSATGQPMRFEILLVDPAFE
                     RIVGPMLRNLERLGIDATMRTVDTAQYQNRLDSFDFDMVVTTWGQSMSPGNEQRDFWS
                     CAAAATPGSRNLAGICDPVVDALVDRVIAAESREDLVAATRALDRVLLAGHYVIPHWH
                     SRIIRVAYWDKLAHPDVLPRFGLDLDAWWVDKGKEQQVAEQKRALTQDAAGR"
     gene            complement(5128..6315)
                     /locus_tag="P5558_15495"
     CDS             complement(5128..6315)
                     /locus_tag="P5558_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRY25772.1"
                     /translation="MRGAAARRAASADWGTEFPMASSLELNKVIAGILTAGIIASASG
                     IVAGILYGPAEQHGEHAFSVAVSDEPSDVAEEAAVPFETLLASADPAAGEGLTRQCAA
                     CHSFEEGGPDKVGPHLWGVVGRDVAGVGSFSYSDAIQGVEGVWDYDKLNVYLKAPRDY
                     VPGTAMSFAGIKDDQKRADLIAYLRTLSEDPVPLPEAAPVEESAGEVDTAAADGAEAA
                     PAAAEAAAAEEAPAAEAAETETAEAAPATEEAPAAEAAETATAEAAPAAEEAPAAEGA
                     DGMVAMVAAADPAAGQKLFRQCAACHSVEEGGGNRVGPALWGVYDRDVATGEGFDYSP
                     ALQEIPGEWDVAKLDHYLANPKEFAPGNKMAFAGVRKEEHRAALIAYLHSLSASPVPL
                     EGQ"
     gene            6462..7184
                     /locus_tag="P5558_15500"
     CDS             6462..7184
                     /locus_tag="P5558_15500"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRY25773.1"
                     /translation="MVLIPARLRSSRLPDKPLALIKGEPMIVHVWRRAIEAGIGPVVV
                     ACAEPAIAEAITAVGGRAVLTDPELPSGTDRIYAALLEIDPDGAFDRVINLQGDMPTL
                     DPRCLHDVLAPVALPGIDIATLADATDDEAEKTDPNTVKAIISFRSAEIGEALYFTRA
                     NAPWGDGPVYHHIGLYAFTRTALDRFAALPPSPLERRERLEQLRALENGMRIGVKLVQ
                     AVPFGVDTPADLMKARKILGDG"
     gene            7177..8058
                     /locus_tag="P5558_15505"
     CDS             7177..8058
                     /locus_tag="P5558_15505"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRY25774.1"
                     /translation="MDEQANDETVDPAGAHQRIAFQGRPGAYSHMACRQAYPELEPLP
                     CESFESAFAAVRDGLARYAMIPVDNTVAGRVADVHHLMPHCGLHIIGEQFLRVNHHLL
                     AVPGATMATVRHVHSHVHALGQCRKFLNEHGLKPVVAADTAGAAADLARTGDLATAVI
                     ASHLAAEIYGLVSLTADVEDAEHNTTRFLVLAQEPRYPGVKDGPAITSFLFKVRNVPA
                     ALYKAMGGFATNGVNMLKLESYIDPTFQQAQFYADILGHPDDEAVRFAMDELRFFAHH
                     IEVFGVYPTSPFRERFR"
     gene            complement(8055..8657)
                     /locus_tag="P5558_15510"
     CDS             complement(8055..8657)
                     /locus_tag="P5558_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRY25775.1"
                     /translation="MPEPIDFYFDFSSPYAYLASRQIGTLAERWGRAVAWRPMMLGVA
                     FAATGGRPLVEIPLKGDYMKVDLVRTARLAGIPFRLPRRFPLATLAAARAYYWLEETS
                     PTQAVPFAEAAFGAFFAEDRDISDRAVIADLLESAGGDAAAGLAAIATPEVKERLRAA
                     TEEAVARGVFGSPFFFVDGEPFWGFDRLPQIELRLAEIGA"
     gene            complement(8662..9645)
                     /gene="speB"
                     /locus_tag="P5558_15515"
     CDS             complement(8662..9645)
                     /gene="speB"
                     /locus_tag="P5558_15515"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRY25776.1"
                     /translation="MADTGDPHGFVPRSGMDLPRFAGPVSFMRLPEVDLDEPRAAEVQ
                     IGLVGVPWDGGTTNRAGARHGPRQLRDLSTMIRLVNQASGIRPFHQARCADLGDCPVN
                     PASLDDTLAKIAAFYARLGEAGIVPMTAGGDHLISLPVLRGIARGRTLGMIHFDAHTD
                     LYDSYFGGYRYTHGTPFRRACEEGLLDPRRIVQIGIRGTAYDGEDVAWGRANGVRIVM
                     IEELMDRGTDAVMDEAREIVGAGPVYVSFDIDGIDPSMAPGTGTPEIGGFSTHQAQRM
                     VRRLQGLDLVGADLVEVSPPFDPSGQTAWVGVNIMFELLCVLADAVGRRAG"
     gene            complement(9675..>11578)
                     /locus_tag="P5558_15520"
     CDS             complement(9675..>11578)
                     /locus_tag="P5558_15520"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRY25777.1"
                     /translation="ALEPTFGAINLEDIKAPECFVVEEKLKARMKIPVFHDDQHGTAI
                     IVAAAIVNGLLLASKRLEDCRLVASGAGAAALACLDLLVTLGLRLENITVTDIDGVVY
                     KGRTASMDPYKARYAQDTPHRTLGDVIEGADIFLGLSAPNVLKPEMLARMAPDPLVLA
                     LANPVSEIDPELARRTRPDVIVATGRSDHPNQVNNVLCFPFIFRGALDVGATTINREM
                     QRACVDALASLARSTTPDTVASAYGQDSLSFGRDYLIPKPFDPRLIVELPIAVARAAV
                     ESGVARRPITDFRAYRQRLSERVFRSGLLMKPLFERAAGAPQRLVYTDGEEEAVLRAV
                     QVVVDEGLASPVLIGRPEVIAMRIERLGLRLAHGRDFELCNPDSDPRYNDYVAFYQER
                     MGRKGVSPQAARDIVRTRRTVIGAIMIARGEADALLAGPVGSFASHLRHITDVVGLKA
                     TMEEASTIHALVLDEGPLFIGDTNVSEEPDAEEIAATTLAAAGVVRSFGIEPKVALVS
                     ASNFGSREQASARRLRAALEIIRKAEPELDIDGEMQADSALSPVIRDRLMPQSRLVGR
                     ANLLIMPSLDAANIAYNLVKSVTGVVSVGPILIGPRLPAHIVTSSISTRGLVNMSAVA
                     CVQALDPIF"
BASE COUNT         1914 a         4050 c         3892 g         1722 t
ORIGIN      
        1 ccaccgtctg cccggcgcgc acctcgagcg tcacgccgtc gaccgccttg acatgcccga
       61 ccgtgcgcct gagcaggccg cgctggatcg ggaaatgcac cttgatgtcc tggccggcga
      121 ggatcaccgg cgccgagcca tcggcgaccg gcggcgcccc cttgggctcg gcggccagca
      181 ggtggcgcgt gtagctgtgc tgtgcaccgg tgaagatcgc ctcggtcggc cccgactcga
      241 cgatctcgcc ctcggtcatg accgcgacgc gatgcgccac cttgcgcacg atggtgaggt
      301 catgggtgat cagcagcacc gccattccca gccgctcctg cagctcggcg atcagcgcca
      361 ggatctgcgc ctgcactgtt acgtcgagcg ccgtcgtcgg ctcgtcggcg atcaggaggt
      421 cgggctcgtt ggccagcgcc atggcaatca tgacgcgctg ccgctggccg cccgagagct
      481 ggtgcgggta ggacctcagg cgggtctcgg cctcatcgat gcggaccagc tcgagcagct
      541 cgagcacgcg cgcccggatg gcatcgccca tcaggccccg atgcagctcc agcacctcgc
      601 cgatctgctg ctcgaccgtg tgcagcgggt tgagcgaggt catcggctcc tggaagatca
      661 tcgatgcccg gttgccccgc acctgccgca gcacggcatc ggacttgccg agcagctcct
      721 cgccgtccag ccgcacgctg ccggacggat gccaggcttt gggatagggc aggagctgca
      781 ggatcgagag ggcggtgacc gacttgcccg agccgctctc gccgacgagc gccagcgttt
      841 cgccccgctc gatggtgaag ctgaccttgc gcaccgcctc gatctcgagc tcgccggcac
      901 gaaagctcac cgaaagatcg tcgaccgcca ggaggctcat gcgtgccctc gcgcgtgcgg
      961 tgcggcatcg accgccagcg ccgcgaacgt cttgcgcgga tcgaaggcat cgcgaaccgc
     1021 ctcgaagatg aagatcagga gggacagcat gatggcgagc accatgaagc cggaaatgcc
     1081 gagccagggc gcctggaggt tgttcttgcc ctgggccaga agctcgccca gcgacggcga
     1141 gccgggcggc agcccgaagc cgaggaagtc gaggctcgtc agcgtggtga tcgagccgtt
     1201 caggatgaag ggcatgaagg tcagcgacgc gaccatggcg ttcggcagga tgtggcggaa
     1261 catgatcgtc gcgttgccga cgccgagggc gcgggcggcg cggacgaaat ccaggttgcg
     1321 cgcccgcagg aactcggcgc gcaccacgtc gaccagcccc atccagctga acagcagcat
     1381 cagcccgagc agccaccaga agttcggggt gacgatgctg gcgaggatga tcaggagata
     1441 gagggtcggc aggcccgacc agatctccat gaagcgctgg aagccgaggt cgacccagcc
     1501 gccgaaatag ccctgcagcg cccctgccgc gatcccgacg atcgacgaca gcacggtgag
     1561 cgtcagcccg aagagcaccg agatgcgaaa gccgtagatc acccgcgcca ggacgtcgcg
     1621 cgcctggtcg tccgtgccga gcaggttctg ccacgtcggc ggcgagggtg cgggagcatc
     1681 gagatcgcgg acgatcgtgt tgtagctgta ggggattggc ggccagagga tccagccgtt
     1741 ctcctcgatg agctcgcgca cgaacggatc gtggtaatcg gtgtcggtcg ggaaaaagcc
     1801 gccgaactcg gtttccgggt agccgaccag caccggaaaa tagagctgcc cgtcatagga
     1861 gacgagcagc gggcgttcgt tggcgatgaa ttcggcgaac aggctgagca cgaagaccag
     1921 gacgaagatg tgcagtgacc agtagccgcg gcggttgttg cggaaattcc agtagcggcg
     1981 ttcattgatc ggattgagac gcaggcggaa gccgagaagg cggggctcgt gccgcatcac
     2041 cccgccctgc cctcgaaatc gattcgcgga tcgaccagca cataggtcag atcggagatc
     2101 agcttcacca cgagaccgag cagggtgaag atgaagagcg agccgaacat caccggatag
     2161 tcccggcgga gcgcggcctc gtagccgagc agcccgagcc cgtcgagcga gaagatgatc
     2221 tcggtcagga gggcaccggt gaacaggatg ccgatgaacg cccccggaaa gccggcgatg
     2281 acgatgagca tcgagttgcg gaagacgtga tggtagagga cccggcgctc ggtcagcccc
     2341 ttggcccgcg cggtcaccac gtactgcttg ttgatctcgt cgagaaagct gttcttcgtg
     2401 agcatggtga ggccggcgaa ggcaccgatc atcagcgaca gcaggggcag cgtcatgtgc
     2461 cagaaatagt cgacgatgcg catcggccag gagagatccg accagttgtc cgacaccagc
     2521 ccacgcagcg gaaaccagtt gaggtagctg cccccagcga acaggacgat gagcaggatg
     2581 gcgaagagaa agctcgggat ggcgctgccg acgatgacca cgccgctcgt ccacacgtcg
     2641 aagctgctgc cgtcgcgcac cgccttcctg atgccgagcg ggatcgagat caggtaggtg
     2701 agcagggtcg tccagaggcc gagcgagatc gagaccggca gcttctcccc gatcaggccg
     2761 atgacgctct tgtccgagaa gaagctcttg ccgaaatcga gcctgatgta attgccgagc
     2821 atcaggaaga agcgctcgcc ggccggcttg tcgaagccgt acatccgctc gatctcggcg
     2881 acgaagcgcg ggtcgaggcc ctgcgcgccc cgatactggc tgcccggttc ctgcgccggg
     2941 cgccgcgggc cgtccccgcc cccgccggtc agccgggccg tgctcgccac cgccgtgccg
     3001 gagatctcgg cgatcatctg ctcgaccggg ccgccgggtg ctgcctggat gacgacgaaa
     3061 ttgacgacca tgatgccgaa gagcgtcggg atgatcagca gcaggcggcg gacgacgtag
     3121 gccagcattc gctgcagccg gctccgctac cggccggcgg cgtcttgcgt cagcgcccgc
     3181 ttctgctcgg cgacctgctg ctccttgccc ttgtcgaccc accaggcatc caggtcgagg
     3241 ccgaagcgcg gcaagacgtc cggatgggcg agcttgtccc agtaggcgac ccggatgatg
     3301 cggctgtgcc agtgcggaat gacgtagtgg cccgccagca gcacacggtc gagggcgcgc
     3361 gtcgcggcca ccagatcctc gcgcgactcg gcggcgatga cgcgatcgac gagggcatcg
     3421 accaccgggt cgcagatgcc cgccaggttg cggctgccgg gcgttgccgc cgccgcgcag
     3481 gaccagaagt cccgctgctc gttgcccggg gacatcgact gcccccaggt ggtcacgacc
     3541 atgtcgaaat cgaaggagtc cagccggttc tggtactggg cggtatcgac cgtccgcatc
     3601 gtcgcgtcga tgcccagccg ctcgaggttg cgcagcatcg ggccgacgat ccgctcgaag
     3661 gccgggtcga ccagcaggat ctcgaagcgc atcggttgcc cggtcgcgct gttcacgagc
     3721 ttcctggtgc tccggtcgat ctcccagccg gcctcccgca gcagctccag cgccgtccgc
     3781 aggttgtcgc gcagctcgcc cgagccgtcc gtcaccggcg gctcgtagac ggtggtgaag
     3841 acctccggcg gcagttccgc gcggaacggc tcgaggatcc gcagctcgcg ctcgtccggc
     3901 agctcccgcg ccgccagatc ggtgttggag aaatagctct tggtccgcgc gtactgcccg
     3961 tagaacagcg tctggttgga ccactcgaaa tcgaaggcat aggccagcgc cgcccgcacc
     4021 cgcggatcgg agaagatctc gcgccgcgtg ttgaagacga aaccctgcat gccgtccccg
     4081 gtctcgtcct ggatctcctc cttgacgatg agcccggcat cgatggccgg cccggtgtag
     4141 ccggtcgccc agagccgggc gatgttctcg atgcggatgt cgtactgccc cgccttgaac
     4201 gcctcgagcg ccacgttgcc gtcgcggaaa tactcgtagc gcatgcgatc gaagttgtag
     4261 cggcccttgt tgaccggcag atcctgcccc cagtagtccg ccacccgctc cagggtgagc
     4321 gaccgccccg gatcgaccct ggccacccga taggggccgc tgccgagcag cggctcgagg
     4381 gtcgtcgcct cgaagtcccg cccttcccag aaatgcttcg gcaggatcgg cagctcgccg
     4441 atgatcagcg gcagctcgcg attgagctcg ccgtcgaaat gaaagagaac gcgccgctcg
     4501 ccggccggct ccgcccccgt cacgttggcg tagtaggcgc ggtagagggg atggccccgg
     4561 gtcttgatgg tctcgaggct gaagatcacg tcctcgaccg tcaccggctg gccgtcgtgc
     4621 cagcgcgcct ccggccgcag cgtgaaggcg acccaggacc ggtcctcggg catctcgatg
     4681 gtctcggcca gaaggccgta ggcggcgaac ggctcgtcgc gcgaccgctc cgtcagcgtc
     4741 tcgaagacat tcgccgcgcc agcggcggcc tggccgcgca ggatgaacgg gttcaggctg
     4801 tcgaagctgc cgatcgccga gaagacgatc tcgccgccct tgggcgcagc cgggtcggca
     4861 taggcgaaat gcgtgaagtc cggcggatag gcgagatcgc catgcatggc gacgccgtgc
     4921 gacggcgtgg cggtctccgc ggcaccggcc acggaggcga ctgccagcgt ggcggccagc
     4981 gcgatcattg ccgtacgggt catccgttcc tcacatctgc ggtcccggca ctcccggctc
     5041 tggctttagc ggtgcgccgc aaggatgcaa atgaaggttc ccgcatcaac gggcaatcat
     5101 ctcaccatgc ggacaggaag cggcgcttca ctgcccttcg agcggcacgg gcgaagcgct
     5161 gagcgaatgc agataggcga tcagggccgc ccggtgctct tccttgcgca cgccggcaaa
     5221 ggccatcttg ttgccgggcg cgaattcctt gggattcgcg aggtaatgat ccagcttcgc
     5281 gacgtcccac tcgcccggaa tctcctggag cgccggcgag tagtcgaaac cttcgcccgt
     5341 cgcgacatcg cggtcataga cgccccagag cgccggcccg acccggttgc cgccgccttc
     5401 ctcgacgctg tggcaggcgg cgcactgccg gaagagcttc tgcccggccg ccggatccgc
     5461 cgccgcaacc atggccacca tgccgtccgc gccttccgcc gcaggagcct cctcggccgc
     5521 cggcgcggcc tcggccgttg cggtctccgc cgcctcggca gcaggagcct cctcggtcgc
     5581 cggcgcagcc tccgccgtct cggtctccgc cgcctcggcc gcaggagcct cctcggccgc
     5641 agcggcctcc gcggctgccg gcgccgcctc ggccccgtcc gctgccgccg tgtcaacctc
     5701 gcccgccgac tcctcgaccg gcgcggcctc gggcagcggc acgggatctt ccgaaagcgt
     5761 ccggagatag gcgatcaggt cggcccgctt ctggtcgtcc ttgatgccgg cgaagctcat
     5821 ggcggtgccg ggaacatagt cgcgcggcgc cttcagatac acgttcagct tgtcgtagtc
     5881 ccaaacaccc tccaccccct gaatggcgtc ggagtagctg aagctcccca caccggccac
     5941 gtcgcggccg accacgcccc agagatgcgg gccgaccttg tccggcccgc cctcctcgaa
     6001 gctgtggcag gcggcgcact ggcgggtcag cccctcgccg gcggccgggt cggccgaggc
     6061 gagcagggtc tcgaaaggca ccgctgcctc ctcggccacg tcgctcggct catccgacac
     6121 ggcgacgctg aacgcgtgct ccccgtgttg ctcggccgga ccatagagga tgccggcgac
     6181 gatgcccgac gcggaagcga tgatgccggc cgtcaggatg cccgcgatga ccttgttgag
     6241 ttcgagggat gatgccatag gaaattctgt tccccagtct gccgaggccg cccgtcttgc
     6301 cgccgcaccg cgcacgcgga tgcttccgtc caggcagtgt ggtcggtagc ggaccggggc
     6361 gtgaaccgtc aagccaccac cacgtcgcta ccgagagact taccgaccgg agccgaaaat
     6421 tgagttctac ggaaaccgcc ggcgtcaatt cgtcgcgcaa tgtggtgctc atcccggcac
     6481 gcctgcgctc ctcgcggctg cccgacaagc ccctggccct catcaagggc gagccgatga
     6541 tcgtccacgt ctggcgccgc gccatcgagg ccgggatcgg cccggtggtc gtcgcctgcg
     6601 ccgagccggc aatcgccgag gcgatcacgg cggtgggcgg ccgcgccgtg ctcaccgacc
     6661 ccgagctgcc ctccggcacc gaccggatct acgccgccct cctcgagatc gaccccgacg
     6721 gtgccttcga ccgcgtgatc aacctgcagg gcgacatgcc gacactcgac ccgcgctgcc
     6781 tccacgacgt cctggccccc gtcgcgctcc ccggcatcga catcgcgacg ctcgccgacg
     6841 ccaccgacga cgaggccgag aagaccgacc ccaacacggt caaggccatc atctccttcc
     6901 gttccgccga gatcggcgag gcgctctact tcacccgcgc caacgcgccc tggggcgacg
     6961 gtcccgtcta ccaccatatc ggcctctatg ccttcacccg gacggccctg gaccggttcg
     7021 ccgccctgcc cccgagcccg ctcgagcggc gcgagaggct ggagcagctg cgcgccctcg
     7081 agaacggtat gcgaatcggc gtaaagctcg tacaagccgt gcccttcggg gtcgacaccc
     7141 cggcggatct catgaaggcg cgaaagattc tcggcgatgg atgagcaggc gaatgacgaa
     7201 acggtcgatc cggcaggcgc acaccagcgc atagcgttcc agggccggcc cggcgcctac
     7261 tcgcacatgg cctgccgcca ggcctatccc gaactcgagc ccctgccctg cgaatcgttc
     7321 gaatccgcct tcgccgcggt tcgcgacggc ctcgcccgct acgccatgat cccggtggac
     7381 aacacggtcg ccggccgcgt cgccgacgtg caccacctga tgccgcattg cggcctgcac
     7441 atcatcggcg agcagttcct cagggtgaac caccacctcc tggccgtgcc gggtgcgacc
     7501 atggccacgg tccgccacgt ccacagccat gtccacgcgc tcggccagtg ccgcaagttc
     7561 ctgaacgagc acgggctgaa gccggtggtc gccgccgaca cggcaggtgc cgccgccgac
     7621 ctcgccagga cgggcgacct cgcgaccgcc gtcatcgcct cgcacctcgc cgcggagatc
     7681 tacggcctcg tcagcctgac cgccgatgtc gaggatgccg agcacaacac cacgcgcttc
     7741 ctcgtgctcg cccaggagcc ccgctatccg ggggtcaagg acgggccggc catcaccagc
     7801 ttcctcttca aggtccgcaa cgtgccggcc gcgctctaca aggccatggg cggcttcgcc
     7861 accaatggcg tcaacatgct gaagctcgag agctatatcg acccgacctt ccagcaggcg
     7921 cagttctacg ccgacatcct gggccatccg gacgacgagg cggtgcgctt cgccatggac
     7981 gagctgcgct tcttcgccca ccatatcgag gtcttcggcg tctacccgac ctcaccgttc
     8041 agggaacgct tccgctaggc gccgatctcg gcgagccgca gctcgatctg cggcagccgg
     8101 tcgaagcccc agaagggctc gccgtcgacg aagaagaagg gcgagccgaa gactccccgc
     8161 gccaccgcct cctcggtcgc cgcccgcaac cgctccttga cctccggcgt ggcgatggcg
     8221 gcaagcccgg cggccgcgtc gcctcccgcg ctctccagaa gatcggcgat gacggcccga
     8281 tcgctgatgt cccggtcctc cgcgaagaac gcgccgaacg ccgcctcggc gaacggcacg
     8341 gcctgcgtcg ggctcgtctc ctcgagccag tagtaggcgc gggcggcggc cagcgtggcg
     8401 agcggaaagc ggcgcggcag gcgaaagggg atgccggcga ggcgcgccgt gcgcaccaga
     8461 tccaccttca tgtagtcgcc cttgagggga atctcgacga gcggccggcc gcccgtggcg
     8521 gcgaaggcca cgcccagcat catcggccgc cacgccaccg cgcgtcccca tcgctcggcg
     8581 agagtcccga tctggcggct cgccagatag gcgtaagggc ttgagaaatc gaaataaaag
     8641 tcgatcggct cgggcattgg ctcaacccgc ccgccggccg accgcgtccg ccagcacgca
     8701 aagcagctcg aacatgatgt tgaccccgac ccaggcggtc tgacccgaag gatcgaaggg
     8761 cgggctgacc tcgaccagat cggcgccgac cagatcgagc ccctgcaggc ggcgcaccat
     8821 gcgctgcgcc tgatgggtcg agaagccgcc gatctccggc gtcccggtgc ccggcgccat
     8881 cgacgggtcg atgccgtcga tgtcgaagct cacatagacc ggcccggcgc cgacgatttc
     8941 ccgcgcctcg tccatcaccg cgtcggtgcc gcggtccatc agctcctcga tcatgacgat
     9001 gcggaccccg ttggccctgc cccaggcgac gtcctcgccg tcataggccg tgccgcgaat
     9061 gccgatctgg acgatacgcc tgggatcgag cagcccctcc tcgcaggcgc gccggaaggg
     9121 cgtgccgtgg gtgtagcgat agccgccgaa atagctgtcg tagaggtcgg tatgggcatc
     9181 gaagtggatc atgccgaggg tccggccccg ggcgatgccg cgcagcaccg gcagggagat
     9241 caggtggtcg ccgcccgccg tcatcggcac gatgccggcc tcgccgagac gcgcatagaa
     9301 ggcggcgatc ttggcgagcg tgtcgtcgag gctcgccggg ttgaccgggc aatcgccgag
     9361 atcggcgcag cgcgcctggt ggaacggcct gatgccgctc gcctggttga cgaggcggat
     9421 catggtcgag agatcgcgca actggcgggg gccatggcgg gcaccggccc ggttcgtcgt
     9481 gccgccgtcc cagggcacgc cgaccaggcc gatctgcacc tcggctgcgc gcggctcgtc
     9541 gaggtccacc tccggcaggc gcatgaagct gaccggcccg gcgaagcgcg gcaggtccat
     9601 gccggagcgc ggcacgaagc cgtgggggtc gcctgtatcc gccatgcacg ccactccagg
     9661 ttctgttgcg ggcttcagaa gatcgggtcc agcgcctgca cgcaggcgac cgcgctcatg
     9721 ttgaccagac cgcgcgtcga gatcgacgag gtgacgatat gcgccggcag gcgcgggcct
     9781 atcaggatcg gcccgaccga gacgacaccc gtcaccgact tcacgaggtt gtaggcgatg
     9841 ttcgccgcgt ccagggacgg catgatcagg aggttggcgc ggccgaccag ccgcgactgc
     9901 ggcatcaggc gatcgcggat caccggcgag agggcactgt cggcctgcat ctcgccgtcg
     9961 atgtccagct ccggctcggc cttgcggatg atctcgaggg cggcccgcag gcgccgtgcc
    10021 gaagcctgct cgcgactgcc gaaattgctc gcggagacga gggccacctt cggctcgatg
    10081 ccgaagctgc gcacaacccc cgccgccgcc agcgtcgtcg cggcgatctc ctcggcatcc
    10141 ggctcctccg aaacattggt atcgccgatg aagagcggcc cctcgtcgag caccagcgca
    10201 tgaatcgtgg aagcctcctc catcgtcgcc ttcagcccga ccacgtcggt gatgtgccga
    10261 agatggctgg cgaacgagcc gaccgggccg gcgagcagcg catcggcctc gccccttgcg
    10321 atcatgatcg ccccgatcac cgtccgccgc gtgcggacga tgtcgcgcgc cgcttgcggg
    10381 ctgacaccct tgcggcccat gcgctcctga tagaacgcca cgtagtcgtt gtagcgcggg
    10441 tcgctgtcgg ggttgcagag ctcgaaatcg cggccgtggg cgagccgcag cccgagccgc
    10501 tcgatccgca tggcgatgac ctcggggcgg ccgatgagca ccgggctggc aaggccctcg
    10561 tccacgacca cctgcaccgc ccggagcacc gcctcctcct cgccgtccgt gtagacgagc
    10621 cgctgtggtg ccccggcggc ccgctcgaaa aggggcttca tcaggagccc cgagcggaag
    10681 acccgctcgg acagccgctg ccgataggcg cgaaagtcgg tgatcgggcg ccgggcgact
    10741 ccggactcca cggccgccct ggccaccgcg atcggcagct cgacgatcag ccgcggatcg
    10801 aagggcttgg ggatgagata gtcgcgcccg aacgacaggc tgtcctggcc ataggcggag
    10861 gccaccgtgt ccggggtcgt gctgcgcgcg aggctggcga gcgcgtcgac gcaggcgcgc
    10921 tgcatctccc ggttgatcgt ggtcgccccg acatcgagcg cgcccctgaa gatgaagggg
    10981 aagcagagga cgttgttgac ctggttcgga tggtcgctgc ggccggtggc gacgatgacg
    11041 tccggccggg tccgccgtgc cagctccggg tcgatctccg agaccgggtt ggccagcgcc
    11101 agcaccaggg gatccggcgc catgcgcgcc agcatctcgg gcttcagcac gttgggtgcg
    11161 gaaaggccga ggaagatgtc cgccccctcg atcacgtcgc ccagcgtgcg gtgcggcgtg
    11221 tcctgcgcgt agcgcgcctt gtaggggtcc atggaagcgg tgcggccctt gtagacgacg
    11281 ccgtcgatgt cggtgaccgt gatgttctcg agccggagcc cgagtgtcac cagcagatcg
    11341 aggcaggcga gcgccgccgc gccggcgccg ctggcgacca ggcggcagtc ctcgaggcgc
    11401 ttgctcgcga gcagcaggcc attgacgatg gcggcggcga cgatgatcgc cgtgccgtgc
    11461 tggtcgtcgt ggaagacggg aatcttcatc cgcgccttca gcttctcctc gacgacgaag
    11521 cactccggcg ccttgatgtc ctcgagattg atcgccccga aggtcggctc gagcgccg
//
LOCUS       DAOTFI010000155        11572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6630_length_11572_cov_7.217070, whole genome shotgun sequence.
ACCESSION   DAOTFI010000155 DAOTFI010000000
VERSION     DAOTFI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6630_length_11572_cov_7.217070"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="P5558_15525"
     CDS             <1..509
                     /locus_tag="P5558_15525"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HRY25778.1"
                     /translation="SYFLPVPFFADSIEDKAVLMAQRSVKDVFALARLADLVVVGIGE
                     LAADAHLLQTGMITQAEFAALAKAGAVGEVLGHFFAADGRSVAAEINGRALGLGPDDL
                     RGKEVTAIAGGRGKARAIHAVLQSGLLTGLVTDEATARRLAELDRGRPPGIGAGAGSP
                     TALLRTAS"
     gene            543..1979
                     /locus_tag="P5558_15530"
     CDS             543..1979
                     /locus_tag="P5558_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25779.1"
                     /translation="MYSRIQDLANAVVRRQLDRRNFLRRAGQLGIGASAATYFLNRAQ
                     STALAQDFDWQKHKGTTVKLLLNKHPYADAMIANLDTFKSMTGMEVTYDVFPEDVYFD
                     KVTAELSSGGGNYDAFMTGAYQTWQYGPAGWLVDMNEFINDAALTNPNYNWEDILPNL
                     RASTAWSGVPGAELGGEGAKQWAIPWGFELNSIAYNKRVFDAQGLTPPNNMAELLEVA
                     AKISKDVDGMYGVGVRGSRSWATIHPGFLSGYSNFNERDFTIEDGKLMAAMATPGSKS
                     FHADWVKMIQQSGPRDWATYTWYQVGTDLGAGASGMIYDADILGYFMNGEGNAEAGNL
                     AYHAFAPNPEASAPTPNVWIWSLGMSNFSTQKEAAWLFMQWASGTDHALFGARDMDFV
                     NPVRQSVWDDQAFQDRIAKSYPGYLEQYKASAPGSKIYFTPQPLFFNLTTEWASMLQQ
                     MVGNEVPVDEGLDQLAESVNRQLRQAGI"
     gene            2051..2980
                     /locus_tag="P5558_15535"
     CDS             2051..2980
                     /locus_tag="P5558_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25780.1"
                     /translation="MATEALGRPVMDGSRRPFMRRALPYLLSLPALLVCIGILIPFGT
                     AVYYSLQRYNLAFPMTRGFIWFENYTLLFTDPGFWNTVRVSLLYTLYTVGFELVLGLA
                     IAMLLQRRSFFNNTMSILLLLPLMIAPAIAALMWKLMTNPGFGILSYFASLLGFPDFK
                     WASASSSALFTVVLIDIWVYTPFMMVLLLAGLRALPRQPFEAAELDGVPQSFVFFRIT
                     LPMLTPYIITASLFRMLESIQQFDIIYAMTQGGPGNSLMVFQVAAYLERYQYTNVGRS
                     AAILMVLWAITFLLSNIFIKQWLRLRERARGVA"
     gene            2980..3837
                     /locus_tag="P5558_15540"
     CDS             2980..3837
                     /locus_tag="P5558_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25781.1"
                     /translation="MQRQTVIGRGVRVVLLTLVGLFFLFPIFWVLLMSFQTNQDILRI
                     PPSLVFTPTLENYGALISGRLESTAGVLEIRFMNNLMNSVILAVSSVALSLLLGIPAA
                     YAFARFKFRLGEDIAFTLLSFRFAPPLLVLLPLALYFQQMGLIDTYIGLIWVYQLITL
                     PLILWIVRGYFEDISEDIEHAYRLDGNGWFKTFTRIAIPLAKPGIAAATLLSFIFAWN
                     NFVFALILASADKQPVTVGALAFVTASGIQYGQIAASIVLSVTPTLLLALYAQRYLVE
                     GLSLGAVKG"
     gene            3848..4972
                     /locus_tag="P5558_15545"
     CDS             3848..4972
                     /locus_tag="P5558_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25782.1"
                     /translation="MSYLSLGKLKKRFKNEVVLDGASLDVERGETVVVFGPSGCGKTV
                     LLRLIAGINEPDSGTVHIAGQEVTYLAPEHRDVGMAFQNFALYPHLSAFDNIASPLSA
                     RGVARGEIERRVHEVAGLLKIDHVLHHLPKALSNGQKQRTSLGRALAGNPGVLLLDDP
                     LRNVDAKLRYEMRLELPRVLKAFETTVIYVTQDYKEAMALGDRVAVLMGGGFRQVDRP
                     ASIYKDPDGTDVARLFGDPTVNLVPGTIEGGTVTALGARFPLAVSLADGPCQIAIRPE
                     SITVTLDEATSAGATPVDLGIGEDTHAVPVELDAVTPLNVRAVMLLRTADGRELLATC
                     SEDEASRLPRGHGRGHAHIRAADLLLFDPAGRRVRPGERQ"
     gene            4972..6099
                     /locus_tag="P5558_15550"
     CDS             4972..6099
                     /locus_tag="P5558_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25783.1"
                     /translation="MATLTLDSVSKVYKAARGRPVTAVDAVDMRIADGEIIGLLGSSG
                     CGKTSTLRMIAGFEDVTSGHIEVGGRVIDNLLPAERNIAMAFEGYALYPPLRVRDNIG
                     FALLRDRQPRAEVKRQVEEIAELLEIVPALDRYPPTISAGQQQRTSLARALIRKADVF
                     LLDEPMSQLEPQLRAVLRARLKDWLITRRMTTVFVTHDQTEAIALADRIAVMEEGVLQ
                     QFASPDELKERPANLFVASFIGEPPMNLFEARVDRSRSGLGLAVLDAGGARAFELRFD
                     AEVAGLADGAAVHLGMRPHRLRLGDRAGAVQGTVTSHSWLGDQTHVGVEIGGTECIVV
                     AEGEVPARLGAPIAVEVPLRAVHLFDADDGRALFHGSEAAS"
     gene            6096..7658
                     /locus_tag="P5558_15555"
     CDS             6096..7658
                     /locus_tag="P5558_15555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRY25784.1"
                     /translation="MTGAGELIIGLDAGTSVIKAVAFDTAGRQVAVAARPNVYDQRPG
                     GVAEQDMARTWTTAAEVLRLLGEEVPDLPRRTAALAVTGQGDGTWLIDEKGEPVAPGW
                     LWLDSRAAGIVDELERSGARRAAWRYTGCGLNACQQNAHLLWLRRHQPETLARAATAF
                     HCKDWLHLQLTGVRATDRSEGTFTFGDFRTREYADEVLDALGLADLRHLLPPMLDGST
                     TTHPLTDEAAAATGLRAGTPVALGFLDVVCTALGAGIYDAERRVGCSIVGSTGMHMRF
                     QPDAGQVELAPVPSGYTMPFPVPGSVVQMQSNMAATINVDWITDRVREAASLFGVEAG
                     RSEALKLLDGRVLDGRPGAALYHPYILEAGERGPFVDSDARAMFSGLSNRVGLLDLVR
                     AVYEGLAFAARDCYAAMGHVPEEIRIAGGAARSRAFKKIMASTLGVPLRESSREEAGA
                     AGAAMMAACAIGMHPDMASCAASWVTPTLGDTVAPDPELVALYDRLFPVYRATREAMP
                     GVWRQLAAVERP"
     gene            7655..8683
                     /locus_tag="P5558_15560"
     CDS             7655..8683
                     /locus_tag="P5558_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRY25785.1"
                     /translation="MTRVSIIGDRFMAPEVFEAALPKAGLDCRLHQLAWPDEPFVNAC
                     ETGELAGLKEFMGEPGQIASMIGDAEVLVTHLAPVSASILERCPSLRLIGVSRGGPVN
                     IDRAACAARAVQVVNAPGRNASAVAEFTIGAILMETRLMRAGHEALRQGIWRGDLYRA
                     DLTGPELSELTVGLIGYGQIGTRVVKLLRPFGCRILVADPYKTLDADDAAHGVVMTDL
                     ADLLARSDVVSLHARVTDETRGFLAAPEFAAMQRGAWFINTARGPMVNYPDLCAALES
                     GHLRGAMLETFWQEPPPADSPLLRLPNVTLTPHIAGASTTTVRIAAAMVAEEIRRWRA
                     GEPFLNPC"
     gene            8700..10226
                     /locus_tag="P5558_15565"
     CDS             8700..10226
                     /locus_tag="P5558_15565"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRY25786.1"
                     /translation="MTEQVIDLLVIGGGINGAGIARDAAGRGLSVTLCEMDDLAQGTS
                     SRSGKLVHGGLRYLEYYEFRLVREALIEREVLLAAAPHIIWPMRFVLPHSREQRPAWL
                     IRLGLFLYDHLGGRKRLPPTRHLDLRRTPEGAPIRKEFTRAFEYSDCWVDDARLVLLN
                     AQGAHERGAEILTRTACVAARRDGDHWQVDLEDRQNGSRRTVRARAIVNAAGPWVERM
                     LGRVAGAASRRRVRLVKGSHIVTRKFWDGPHAYLLQNTDKRVIFVNPYEGDLALIGTT
                     DIPVEGDPGSVAIDQAEIDYLLGVINRYFEQQLTPADIVTTFSGVRPLYDDAAENPSA
                     VTRDYVFDVEPAAPTGGQPPILSVFGGKITTYRKLAEHALDKLQPFFPTLGPAWTAKA
                     PLPGGDLPDADFDRFLNDLAHEFTWLPAELVLHYARLYGTRARVLLDGAAAMADLGRH
                     FGGLLHEREVAYLRRHEWALTAADILDRRTKHGLHLTPAERRAFEAWCDERQAAPSDA
                     "
     gene            10219..11454
                     /locus_tag="P5558_15570"
     CDS             10219..11454
                     /locus_tag="P5558_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRY25787.1"
                     /translation="MPEFPCTLGIDCGTSGLRGVVVDAAGTVLATAHRPFPAGTTDPA
                     LWASAMDAVIAELAPTAPVAALAIDGTSGTLLLTDAAGNPLGPALLYNDASAADLAPR
                     IATLAPPESAAHGATSPAARLRRLQEGHPEAVHALHQADWLAARLTGRLGLSDDNNAL
                     KLGWDPVRRTWPAWLEAFGIRTDLLPEIVEPGTPLGEVRHGPLEGSRVTAGTTDGCAS
                     FLATGASAPGDGVTALGTTLTLKILSDQPIFAPAHGIYSHKLLGAWLAGGASNSGGAA
                     LLRYFAADEIEALTPSLRPDAPLDLGWHPLPGTGERFPMADPAMTFEPADIPKTRENF
                     LQALMEGIARVEALAYRRLQELGGPPLGSVRTVGGGARNDAWTRIRARHLAVPMLQPP
                     STDAAYGTALLARKGLIDG"
BASE COUNT         1685 a         4518 c         3597 g         1772 t
ORIGIN      
        1 agagctactt cctgccggtg ccgttcttcg ccgacagcat cgaggacaag gcggtcctga
       61 tggcgcagcg cagcgtcaag gacgtgttcg cgctcgcccg cctcgccgac ctcgtcgtcg
      121 tcggcatcgg cgagctcgcc gccgacgcgc acctcctgca gaccggcatg atcacgcagg
      181 ccgagttcgc cgcgctggcg aaggccggtg ccgtgggcga ggtgctcggc cacttcttcg
      241 ccgccgacgg ccgctcggtc gccgccgaga tcaacggccg ggcgctcggc ctcgggcccg
      301 acgacctgcg cggcaaggag gtcaccgcca tcgccggcgg ccggggcaag gcccgggcga
      361 tccacgccgt gctgcagagc ggcctgctca ccggcctcgt caccgacgag gcgaccgcgc
      421 gccgactcgc cgagctcgat cgcggccggc ccccgggcat cggagccggt gccggctcgc
      481 cgacagcgtt gttgagaacc gcgtcctgac cggggcgcgt gccaccaagg gaggagaagg
      541 gaatgtactc gaggatccag gatctcgcca atgccgtggt caggcgccag ctcgaccgcc
      601 gcaacttcct gcgccgcgcc ggccagctcg gcatcggcgc cagcgccgcc acctacttcc
      661 tgaaccgggc gcagagcacc gccctggccc aggacttcga ctggcagaag cacaagggca
      721 cgacggtcaa gctgctcttg aacaagcacc cctacgccga tgcgatgatc gccaacctcg
      781 acaccttcaa gtcgatgacc gggatggagg tcacctatga cgtcttcccc gaggacgtct
      841 atttcgacaa ggtgacggcc gagctctcgt cgggcggcgg caattacgac gccttcatga
      901 ccggcgccta ccagacctgg cagtacggcc cggccggctg gctggtcgac atgaacgagt
      961 tcatcaacga cgcggccttg accaacccca actacaactg ggaagacatc ctgccgaacc
     1021 tgcgcgcctc caccgcctgg tcgggcgtgc cgggtgccga gctcggcggc gagggggcca
     1081 agcagtgggc gatcccctgg ggcttcgagc tcaactcgat cgcctacaac aagcgggtct
     1141 tcgatgccca gggcctgacg ccgcccaaca acatggccga gctgctggag gtcgcagcga
     1201 agatcagcaa ggatgtcgac ggcatgtacg gcgtcggcgt gcgcggctcg cgctcctggg
     1261 ccaccatcca cccgggcttc ctctccggct actcgaactt caacgagcgc gacttcacca
     1321 tcgaggacgg caagctcatg gccgccatgg cgacgccggg ctccaagtcg ttccatgccg
     1381 actgggtgaa gatgatccag cagagcgggc cgcgcgactg ggcgacctac acctggtacc
     1441 aggtcggcac cgacctcggc gccggcgctt ccggcatgat ctacgacgcc gatatcctgg
     1501 gatacttcat gaacggcgag ggcaatgccg aggcgggcaa cctcgcctac cacgccttcg
     1561 cgccgaaccc ggaggcgtcc gcgccgacac ccaacgtctg gatctggtcg ctcggcatga
     1621 gcaacttctc gacccagaag gaggccgcct ggctcttcat gcagtgggcc tcgggcaccg
     1681 accatgcgct cttcggcgcc cgcgacatgg acttcgtcaa cccggtccgc cagtcggtct
     1741 gggacgacca ggccttccag gaccggatcg ccaagagcta cccgggctat ctcgagcagt
     1801 acaaggcctc ggccccgggc tcgaagatct acttcacgcc gcagcccctc ttcttcaacc
     1861 tgacgaccga gtgggcgtcg atgctgcagc agatggtcgg caacgaggtg ccggtcgacg
     1921 agggcctcga ccagctcgcc gagagcgtca accgccagct ccggcaggcg ggcatctgat
     1981 cgaccactga acgggccggg gccggcaccg accggccccg gcgcctcatc gagctcgagg
     2041 ggaagcagcg ttggcaaccg aggcactggg caggccggtc atggacggct cgcgtcggcc
     2101 gttcatgcgg cgggccttgc cctatctcct gagcctgccg gcgctgctcg tctgcatcgg
     2161 catcctcatc ccgttcggca cggcggtcta ctactcgctg cagcgctaca acctcgcctt
     2221 cccgatgacc cgcggcttca tctggttcga gaactacacg ctcctcttca ccgatccggg
     2281 cttctggaac acggtccggg tctcgctgct ctacacgctc tacacggtcg gtttcgagct
     2341 ggtcctcggg ctcgccatcg cgatgctcct gcaaaggcgc tcgttcttca acaacacgat
     2401 gagcatcctg ctgctgctgc ccttgatgat cgcgccggcg atcgccgccc tcatgtggaa
     2461 gctgatgacc aacccgggct tcggcatcct cagctatttc gcgagccttt tgggctttcc
     2521 cgacttcaag tgggcgagcg cctcctcctc cgccctcttc accgtcgtcc tcatcgacat
     2581 ctgggtctac acgcccttca tgatggtgct gctgctcgcc gggctccgcg ccctgccgcg
     2641 ccagcccttc gaggcggccg agctcgacgg ggtgccgcag agcttcgtct tcttccgcat
     2701 caccctgccg atgctcaccc cctacatcat caccgccagc ctctttcgca tgctcgagag
     2761 catccagcag ttcgacatca tctacgcgat gacccagggc gggccgggca actcgctcat
     2821 ggtcttccag gtggcggcct atctcgagcg ctaccagtac accaatgtcg gccgctcggc
     2881 ggcgatcctc atggtgctct gggcgatcac cttcctgctc tcgaacatct tcatcaagca
     2941 gtggctgcgc ctgcgcgagc gcgcccgggg agtcgcctga tgcagcgcca gaccgtgatc
     3001 ggccggggcg tgcgggtggt gctgctcacc ctggtcgggc tcttcttcct cttcccgatc
     3061 ttctgggtgc tcctgatgtc cttccagacc aaccaggaca tcctgcgcat ccccccgtcg
     3121 ctcgtcttca ccccgacgct cgagaattat ggggcgctca tctcgggccg gctggagagc
     3181 accgcgggcg tgctcgagat ccgcttcatg aacaacctca tgaacagcgt gatcctcgcc
     3241 gtctcgtcgg tcgccctctc gctgctgctc ggcatcccgg cggcctatgc cttcgcccgg
     3301 ttcaagttcc gcttgggcga ggacatcgcc ttcacgctcc tctcgttccg cttcgccccg
     3361 ccgctcctcg tcctcctgcc gctcgccctc tacttccagc agatgggcct gatcgacacc
     3421 tatatcggcc tgatctgggt ctaccagctc atcaccctgc ctttgatcct gtggatcgtg
     3481 cgcggctatt tcgaggacat cagcgaggac atcgagcatg cctaccggct cgatggcaat
     3541 ggctggttca agaccttcac gcgcatcgcc atcccgctgg ccaagccggg catcgccgcg
     3601 gcgacgctgc tctccttcat cttcgcctgg aacaatttcg tcttcgcgct gatcctggcc
     3661 tcggccgaca agcagccggt gaccgtgggg gccctcgcct tcgtcaccgc ctcgggcatc
     3721 cagtacggcc agatcgccgc ctccatcgtg ctctcggtga ccccgacttt gctcctcgcc
     3781 ctctacgccc agcgctacct ggtcgagggc ctctcgctcg gggcggtcaa gggatgaggg
     3841 gcaggagatg agctaccttt ccctcggcaa gctgaagaag cgcttcaaga acgaggtcgt
     3901 gctcgacggc gcgtcgctcg acgtcgagcg gggcgagacg gtcgtggtct tcggcccctc
     3961 cggctgcggc aagacggtgc tgctccgcct gatcgccggc atcaacgagc cggattccgg
     4021 caccgtgcat atcgccggcc aggaggtcac ctacctcgcc ccggagcatc gcgacgtcgg
     4081 catggcgttt cagaacttcg ccctctaccc gcacctctcg gccttcgaca atatcgcgag
     4141 cccgctcagc gctcgcggcg tcgcccgcgg cgagatcgag cgccgggtgc acgaggtggc
     4201 gggcctcttg aagatcgacc acgtcctcca ccatctgccg aaggcgctct ccaacggcca
     4261 gaagcagcgc acctcgctcg gccgggcgct cgccggcaac ccgggcgtgc tgctgctcga
     4321 cgaccccttg cgcaatgtcg acgccaagct gcgctacgag atgcggctcg agctgccgcg
     4381 cgtcctcaag gcgttcgaga cgacggtgat ctacgtgacc caggactaca aggaggccat
     4441 ggcgctcggc gaccgggtgg ccgtgctgat gggcggcggg tttcgccagg tcgaccggcc
     4501 ggcttccatc tacaaggacc cggacggcac cgacgtcgcc cggctattcg gcgaccccac
     4561 cgtcaacctc gtgcccggca cgatcgaggg cggcacggtc accgcgctgg gcgcccgctt
     4621 tcccctggcc gtaagcctcg ccgacggccc ctgccagatc gccatccgcc cggagagcat
     4681 caccgtcacc ctcgacgaag ccacttccgc tggcgccacg ccggtcgatc tcggcatcgg
     4741 cgaggacaca cacgcggtgc cggtcgagct cgacgccgtg accccgctga acgtgcgcgc
     4801 ggtcatgctg ctcaggacgg cggacgggcg cgagcttttg gccacctgct cggaggacga
     4861 ggcgagccgg ctgccgcgcg gccacggccg gggccatgcc catatccgcg ccgccgacct
     4921 cctcctcttc gatcccgccg gccgacgcgt gcgacccggg gagcggcagt aatggcgaca
     4981 ctcaccctcg attccgtctc caaggtctac aaggccgccc gcggccggcc ggtcacggcc
     5041 gtcgacgcgg tcgacatgag gatcgccgac ggcgagatca tcggcctgct cggctcatcg
     5101 ggctgcggca agaccagcac gcttcggatg atcgccggct tcgaggacgt cacctccggc
     5161 catatcgagg tcggcgggcg ggtgatcgac aatctcctgc cggccgagcg caacatcgcc
     5221 atggccttcg agggctatgc gctctacccg ccgctccgcg tgcgcgacaa tatcggcttc
     5281 gcgctcctgc gcgaccgcca gccccgggcc gaggtcaagc ggcaggtcga ggagatcgcc
     5341 gagctgctcg agatcgtgcc ggccctcgac cgctacccgc ccaccatctc cgccgggcag
     5401 cagcagcgca ccagcctcgc ccgggcgctg atccgcaagg ccgacgtctt cctcctcgac
     5461 gagcccatgt cgcagctcga gccgcagctc cgcgccgtgc tccgcgcccg cctcaaggac
     5521 tggctgatca cccgcaggat gaccaccgtc ttcgtcaccc acgaccagac cgaggccatt
     5581 gcgcttgccg accgcatcgc cgtcatggag gagggtgtcc tgcagcagtt cgcctccccc
     5641 gacgagctga aggagcggcc ggccaacctc ttcgtcgcct ccttcatcgg cgagccgccc
     5701 atgaacctct tcgaggccag ggtcgaccgc agccgttcgg gcctcggtct cgccgtgctc
     5761 gacgccggtg gcgccagggc cttcgagctg cgtttcgacg ccgaggtggc cgggcttgcc
     5821 gatggtgccg cggtgcatct cggcatgcgt ccgcatcggc tccgcctcgg cgatcgcgcg
     5881 ggtgccgtcc agggcacggt gaccagccat agctggctcg gcgaccagac ccatgtcggc
     5941 gtcgagatcg gcggcacgga atgcatcgtc gtggccgagg gcgaggtgcc ggcccgcctc
     6001 ggtgcgccga tcgccgtcga ggtgccgctg cgcgcggtcc atctcttcga cgcggatgac
     6061 gggcgggccc tctttcacgg gtcggaggcc gcgtcttgac cggggcaggc gagctgatca
     6121 tcggcctcga tgccggcacc tccgtgatca aggccgtggc cttcgacacg gccggccggc
     6181 aggtcgccgt ggccgcccgg cccaacgtct atgaccagcg gccgggcggc gtcgccgagc
     6241 aggacatggc ccgcacctgg acgaccgccg ccgaggtcct gcgcctgctc ggcgaggagg
     6301 tgcccgacct gccccgccgg accgctgccc tcgcggtcac tggccagggc gacggcacct
     6361 ggctgatcga cgagaagggc gagcccgtgg ccccgggctg gctctggctc gacagccggg
     6421 ccgccggaat cgtggacgag ctcgagcgct ccggcgcccg ccgcgccgcc tggcgataca
     6481 ccggctgcgg cctcaatgcc tgccagcaga acgcccacct cctctggctg cgacgccacc
     6541 agcccgagac cctggcgcgc gccgccaccg ccttccactg caaggactgg ctgcatctgc
     6601 agctcaccgg cgtgcgcgcc accgaccgct cggaaggcac tttcaccttc ggcgatttcc
     6661 gcacccgcga atatgcggac gaagtgctgg acgccctggg cctcgccgat ctgcgccacc
     6721 tcctgccgcc gatgctcgac ggctcgacca cgacccatcc gctgacggat gaagccgccg
     6781 ccgccaccgg gctccgggcc ggcacgcctg tggccctcgg cttcctcgac gtcgtctgca
     6841 ccgcgctcgg ggccggcatc tacgatgccg agcgccgggt cggctgctcg atcgtgggat
     6901 ccacgggcat gcacatgcgc ttccagcccg acgccgggca ggtcgagctg gcaccggtgc
     6961 cctcgggcta caccatgccg tttccggtgc cgggctcggt cgtgcagatg cagtccaaca
     7021 tggccgccac catcaatgtc gactggatca ccgaccgggt gcgcgaggcc gcctccctct
     7081 tcggcgtcga ggccggccgt agcgaggcgc tgaagctgct cgacggccgc gtgctggacg
     7141 gccgcccggg ggcagccctc taccacccct acatcctcga ggccggcgag cgcggcccct
     7201 tcgtcgacag cgacgcgcgc gccatgttct ccgggctctc gaaccgggta ggcctcctcg
     7261 acctggtgcg ggcggtctac gagggcctcg ccttcgccgc ccgcgattgc tacgccgcca
     7321 tgggccacgt gcccgaggag atccgcatcg ccggtggtgc tgcccgctcg cgcgccttca
     7381 agaagatcat ggcgagcacc ctcggcgtgc cgctccgcga gagcagccgc gaggaggccg
     7441 gtgctgccgg tgccgccatg atggccgcct gcgccatcgg catgcacccc gacatggcga
     7501 gctgtgccgc gagctgggtg acaccgacgc tcggcgacac cgtggcaccg gacccggagc
     7561 tcgttgccct ctacgaccgg ctcttccccg tctaccgggc gacgcgcgag gcgatgccgg
     7621 gcgtgtggcg gcaactcgca gccgtggagc ggccatgacc cgcgtttcga tcatcggcga
     7681 ccgcttcatg gcgcccgagg tgttcgaggc agctttgccg aaagccggcc tcgactgccg
     7741 cctgcaccag ctcgcctggc ccgacgagcc cttcgtcaat gcctgcgaaa ccggcgagct
     7801 cgccgggctc aaggagttca tgggcgagcc gggccagatc gcgtcgatga tcggcgacgc
     7861 cgaggtgctg gtcacgcacc tcgcccccgt ctccgcctcg atcctcgagc gctgcccgag
     7921 cctccgcctg atcggggtca gccgcggcgg ccccgtcaat atcgaccgcg ccgcctgcgc
     7981 cgcccgggct gtgcaggtcg tcaacgcccc cggccgcaac gcctcggccg tggccgagtt
     8041 caccatcggc gccatcctca tggaaacccg cctgatgcgc gccggccacg aggcgctccg
     8101 ccagggcatc tggcgcggcg acctctaccg cgccgacctc accggccccg agctctccga
     8161 gctcaccgtc ggcctcatcg gctacggcca gatcggcacc cgtgtcgtca agctgctccg
     8221 acccttcggc tgccgcatcc tggtggccga cccctacaag acgctcgatg ccgacgatgc
     8281 cgcgcatggc gtcgtcatga ccgacctcgc ggacctcctg gcgcgctcgg atgtcgtcag
     8341 cctgcacgcc cgcgtcaccg acgagacacg cggcttcctc gccgccccgg agttcgccgc
     8401 catgcaacgc ggcgcctggt tcatcaacac cgcccgcggg ccgatggtga actatcccga
     8461 cctctgcgcg gccctcgaat ccgggcacct gcgcggcgcc atgctcgaga ccttctggca
     8521 ggagccgccg ccggccgatt cgccgctgct caggctcccc aacgtcaccc tgaccccgca
     8581 catcgccggc gcctcgacga ccaccgtgcg catcgccgcc gccatggtcg ccgaggagat
     8641 ccgccgctgg cgcgcgggcg agcccttcct caacccctgc tgatggagcc ctgttgccga
     8701 tgacggaaca ggtcatcgac cttctcgtga tcggcggcgg catcaatggc gccggcatcg
     8761 cccgcgacgc ggccggccgc ggcctcagcg tcaccctgtg cgagatggac gacctggcgc
     8821 agggcacctc ctcgcgctcc ggcaagctcg tccatggcgg gctccgctac ctcgaatatt
     8881 acgagttccg cctcgtccgc gaggcgctga tcgagcgcga ggtcctgctc gccgccgccc
     8941 cgcacatcat ctggcccatg cgcttcgtcc tgccgcacag cagggagcag cgccccgcct
     9001 ggctgatccg cctcggcctc ttcctctacg accacctggg cggccgcaag cgcctgccgc
     9061 cgacccgcca cctcgacctc cgccgcacgc ccgaaggtgc ccccatccgc aaggaattca
     9121 cccgcgcctt cgagtattcc gactgctggg tcgacgacgc ccgcctcgtc ctgctcaacg
     9181 cgcagggcgc gcacgagcgc ggtgcggaga tcctcacccg caccgcctgc gtcgccgcaa
     9241 ggcgcgacgg cgaccactgg caggtggatc tcgaggatcg ccagaacggc agccgccgca
     9301 ccgtccgggc gcgcgccatc gtcaacgccg ccggcccctg ggtcgagcgg atgctcggcc
     9361 gcgtcgcggg tgcggccagc cgccgccgcg tccgcctggt caagggcagc cacatcgtca
     9421 cccgcaagtt ctgggacggc ccccacgcct atctcctgca gaacaccgac aagcgggtga
     9481 tcttcgtcaa cccctacgag ggcgacctcg ccctgatcgg cacgaccgac atcccggtcg
     9541 agggcgaccc cggcagcgtc gccatcgacc aggccgagat cgactacctg ctcggcgtca
     9601 tcaaccgcta cttcgagcag cagctcaccc cggccgacat cgtcaccacc ttctccggcg
     9661 tgcgcccgct ctacgacgac gccgcggaga acccctccgc cgtcacccgc gactacgtct
     9721 tcgacgtcga gcccgcagcc ccgaccggcg gccagccgcc catcctctcg gtcttcggcg
     9781 gcaagatcac gacctacagg aagctcgccg agcacgccct ggacaagctc cagcccttct
     9841 tccccacgct cggcccggcc tggaccgcca aggcacccct gcccggcggc gacctgccgg
     9901 acgccgattt cgaccgcttc ctgaacgacc tcgcccatga gttcacctgg ctcccggccg
     9961 agctggtgct gcactacgcc cggctctacg gcacgcgggc gcgcgtcctg ctcgatggcg
    10021 ccgccgcgat ggccgatctc ggccggcatt tcggcggcct gcttcacgag cgcgaggtcg
    10081 cctatctccg ccgccacgaa tgggcgctca ccgccgccga catcctcgac cggcggacca
    10141 agcacggcct gcacctcacc cccgccgagc gccgggcgtt cgaggcctgg tgcgacgaac
    10201 ggcaggctgc cccctccgat gcctgaattc ccctgcacat tgggcatcga ctgcggcacc
    10261 tccggcctga gaggcgtcgt cgtcgacgcc gccggcacgg tcctcgccac cgcccatcgc
    10321 ccgttccccg ccggcaccac cgacccggcc ctttgggcca gcgccatgga cgcggtcatc
    10381 gccgagctcg cccccaccgc ccccgtcgcc gcgctggcca tcgacggcac ctccggcacg
    10441 ctgctgctca ccgacgccgc cggcaatccg ctcggcccgg cgctcctcta caacgacgcc
    10501 tccgccgccg acctcgcccc ccgaatcgcc acgctcgccc cgccggaaag cgccgcccac
    10561 ggcgccactt cgccggccgc ccgcctgcgc cgcctgcagg agggccatcc cgaagccgtc
    10621 cacgccctcc accaggccga ctggctggcc gcccggctca ccggccgcct gggcctcagc
    10681 gacgacaaca acgcgctcaa gctcggctgg gaccccgtcc ggcggacctg gcccgcctgg
    10741 ctcgaagcct tcggcatccg caccgacctc ctgcccgaaa tcgtcgagcc cggcacgccg
    10801 ctcggcgagg tgcgccacgg cccgctcgag ggctcccgcg tcaccgccgg caccaccgac
    10861 ggctgcgcct ccttcctcgc caccggcgcc tccgccccgg gcgacggtgt gaccgccctc
    10921 ggcaccaccc tcaccctgaa gatcctctcc gaccagccga tcttcgcccc cgcccacggc
    10981 atctacagcc acaagctgct gggcgcctgg ctcgccggcg gcgcctccaa cagcggcggc
    11041 gccgcgctct tgcgctactt cgcggccgac gagatcgaag ccctgacacc aagcctccgc
    11101 cccgacgcgc ccctcgatct cggctggcat cccttgcccg gcacaggcga gcgcttcccc
    11161 atggccgacc ccgccatgac cttcgagccg gccgacatcc cgaaaacgcg ggaaaacttc
    11221 ctccaggccc tcatggaagg catcgcccgc gtcgaggcgc tggcctatcg acggctccag
    11281 gaactcggcg gcccgccgct cggctcggtc cgcacggtcg gcggcggggc caggaacgac
    11341 gcctggaccc gcatccgcgc ccgccacctg gctgtgccga tgctccagcc ccccagcacc
    11401 gacgccgcct acggcaccgc cctcctcgcc cgcaaaggtc tcatcgatgg atgatttgaa
    11461 ggcgctcgcc gaattctcgg ccaggatcgg cagcgacccc gagcaggtgc aggcgggcgg
    11521 cggcaacacc tcgctcaagc tggacggcac gctctgggtc aaggcctcgg gc
//
LOCUS       DAOTFI010000156        11567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6635_length_11567_cov_6.575747, whole genome shotgun sequence.
ACCESSION   DAOTFI010000156 DAOTFI010000000
VERSION     DAOTFI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6635_length_11567_cov_6.575747"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1105
                     /locus_tag="P5558_15575"
     CDS             2..1105
                     /locus_tag="P5558_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25788.1"
                     /translation="MTTAAEEKVAVATQLQLTWWRFRRHKVAVASGVIVLFFYLVAVL
                     ADFLATNDPYATLAARSYIPPQEIHWFDADGSFRPHVFALEGKRDMKTFRLVYVADPE
                     DKRYLELFVDGYPYRLLGLFETNLHLLGLDRGAPEEALFLLGTDQLGRDFWSRLVLAT
                     RTSLFIGLAGVAISLFLGVLLGGISGMYGGWIDTVIQRVIEILRSIPTIPLWMGLAAA
                     LPATWSVLQVYLAITVIISLIGWTELARVVRGRFLSLREEDFVMAAELAGASQLRIIF
                     RHMVPSFLSHIIAAVSLALPAMIISETTLSFLGLGLRPPAISWGVLLQDAQNIQALAI
                     APWLLMAAIPVIVVILAFNFLGDGLRDAADPYG"
     gene            1098..2138
                     /locus_tag="P5558_15580"
     CDS             1098..2138
                     /locus_tag="P5558_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25789.1"
                     /translation="MAEPILSVRRLCTWFHQDEGIVRAVDGASFDLEPGHTLGIVGES
                     GCGKSVTARSILRIVERPGRVESGEILLRRPDGSVVDLVKLDGQGREMREVRGGEIGY
                     VFQEPMSSLSSFHTIGNQLIEAIRLHSDMSKKAARDRAVELLTLVGIPRPAQRVDSYS
                     FELSGGLRQRVMIALALAAEPRILIADEPTTALDVTTQAQILDLLRKLQAEQGLAVIL
                     ITHDMGVIAEMADDVVVMYLGRDVEQGPVETIFHDPAHPYTRSLLQSIPSVLAEPRSR
                     LRTIEGSIPHPYARPAGCPFHPRCADAIAGRCDRSLPEPTHLGARHAVSCFLYGGSGV
                     PALPAGLLERAE"
     gene            2135..3175
                     /locus_tag="P5558_15585"
     CDS             2135..3175
                     /locus_tag="P5558_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY25790.1"
                     /translation="MTAEPQTILEVEGLRKWFPISRGLLRRVVGHVRAVDDVTFSIKH
                     GETLSLVGESGCGKTTTSRCILRALTPTDGAIRFRTETGEQVDVAKLPKAGLRPLRRQ
                     MQMIFQDPFSSLNPRMTITDIIAEPLLVNGVRDLETRRRRVRELLDLVQLPAAYMNRF
                     PHAFSGGQRQRIGIARALALNPALIVADEPVSALDVSVQAQVVNLLLDLQDRLGLSYL
                     FVAHDLSVVKHVSDRVAVMYVGKIVELAPTADLFTRPRHPYTEALLSAVPVPDPRRRT
                     GRIVPLGEVADPANPPSGCHFHPRCRYAVERCRVEIPALRPMADDHLARCHLAEELNL
                     AGVQEGHPPATT"
     gene            3194..4129
                     /locus_tag="P5558_15590"
     CDS             3194..4129
                     /locus_tag="P5558_15590"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY25791.1"
                     /translation="MAASAPRVFFEPKIPDYEPGLYDFERFDEGVPATAAPEALDRFR
                     EDGFLMVRGLLPVEEVVAARAELEAMALSDAPDCWGIWYEGALRDHIRLDPSLDREID
                     GKVGGVGFAPGQEADRLPDVEPDLRARHVRKFMGFFRRHPALGQLARHPAIMALVDRL
                     TGGSSELFQDMALVKPARGREKPWHQDHAYFNVAIDTPIVGVWIPMGEVTPENGCMHM
                     LAGGHKEGPRLHFKRRDWQICDTDVATSGRVAVPMRPGDVLLFDGKIPHGTPTNQTDS
                     FRWAVQYHYRPADAVLIEDAERLEAFGSEGKNVTC"
     gene            4299..5648
                     /locus_tag="P5558_15595"
     CDS             4299..5648
                     /locus_tag="P5558_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25792.1"
                     /translation="MRDLPNTKYDLSRRRLLGSAAGAATLAGMGGLARSPLAATWADR
                     PPSEVDLLNFVVWTYGDIYSQLATRFEEDWGVPVESVISSFNDHPAKLMTMFASGDYI
                     DVSQSSPFSFPNFIQQGLVEPLDGLPGIEEYVADFSPFTKQVAVQNGQVMGLPYFSAI
                     WVWNYYEDLLEQAGLEPFQDYDELIEQLRKAKKDGVSQHPILWVAGVGLEQLPGTWYS
                     LTWNRGGTFFEPDGTPALGPGSVARETLRWWAKTFEEELADPESLKVQFTSSAKAFSA
                     GKNLYRGPNHHYGLSIVNDPEQSPIAGRVKVHGFPGDGRTIGVTHVYFLSTGTRDRDW
                     AWKLLQYLGGRTKDGEYTQAVNLAKDAMLGSGYQSVMESDIIRESWAQWGDVDAILDM
                     WGKATYLGEVVSSVNEPWHFPWTDRLNIEVQNCLTRQIDADQCCDILTAAVDEAKRG"
     gene            5765..6619
                     /locus_tag="P5558_15600"
     CDS             5765..6619
                     /locus_tag="P5558_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25793.1"
                     /translation="MNVPTILILALVLAYPIYYAGFLSFHQVGPGQLRSGVFPFVGLL
                     NYERVLTDPLFQKALLNTFTFSAIVVVAEIVLAVAIALLINQQGIWTSRVTRILMLVP
                     YAVPPIANGLVWSFMYSYQFGFLNRILFTLGVITEPINWAGNPDTALFAITVPYVWRT
                     LPFAILLVHAALQGINKELHEAAAVDGANAWRRFWHITLPLLTPIIVVILVLRTSFAF
                     AVFEEILAITQGGPGDATWVAAWYSYRKSFAPPFDIGLGSASAYLLAMLVGILALVYV
                     RMIYRRVE"
     gene            6624..7511
                     /locus_tag="P5558_15605"
     CDS             6624..7511
                     /locus_tag="P5558_15605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25794.1"
                     /translation="MLKPSPIAQILLHFANVLVIAFFLLPIVAVTIGSLQSEKMLQAD
                     TQAILPAEWTFDNFLVILSGGEQRGKIFEQVTYLPDNVKSFHRAFANSVIVATSVTIL
                     TLVFGSLSAYTVARLRFGWTIWLMNLNVMARFVPIIVLMIPLYVTFRNFGMLNSLSGV
                     IVALTGFLLPYAILMLAPYFATIPRELEDAARIDGCTHFSAFWRITLPLSAPGLASCG
                     AIMFIISWHELLIPLILNSRAEFMTLPVVIASLVGDVHVFFNLMMAICLLAMLPSVIL
                     VLLLQRFVVQGLQVGGVKG"
     gene            7515..7820
                     /locus_tag="P5558_15610"
     CDS             7515..7820
                     /locus_tag="P5558_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25795.1"
                     /translation="MYSFDKSRLDLAREFKANPFGEHRPDLQYLLNLMRRPDPKRPHF
                     VLMPRQWGESWTLAVMEPGGTSPPRPTNLVFTDAGEAEWHVFKLRWAELAGEELPFD"
     gene            7884..10148
                     /locus_tag="P5558_15615"
     CDS             7884..10148
                     /locus_tag="P5558_15615"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase large subunit"
                     /protein_id="HRY25796.1"
                     /translation="MTRIMGYCDRWSVRPGDTVNFMVSCIGAESYAAEIVRLKQPEAG
                     PLATPFEPEPVASDVNGRYAGREQALPIGSLAVVTGHPCLERLEAATLVAYVMPTTPS
                     KGRQALFGNWCEASRTGFGLELDEQGSLVLRLGAGRGTEVTLATGEPMRAWRWYRVAG
                     TFDSQSGRMAIWQEPVSRHGFDLDRPIARSAERGCPSTRSMNPLTFAAWQNGETAGPS
                     AWGGLQFAQHFNGRIDRPRIVEGALDHAGIAELMGETGPAAIEHELVAAWDFSLDIGG
                     EAIIDTGPNRLHGTIVNLPTRAVHGHNWTGCINDWTQAPEQYGAIHFHDDDIVDACWT
                     VDFSFEVTSAQRSGVYAAKLTAGDFVFWVPFFVSPPKGEARSKVAFIASTATYTVYLN
                     NKGRFLSSATEVYQGRVTVMDEIDSLLFEYPAMGLSTYDRHSDGTGVAYASRHRPVHN
                     FRPTGRHWNFNLDLCIIDWLEHLGGDYDVITEEQLHEEGMGLLAPYNVVITGSHPEYD
                     SLDMLDAFQAYLRRGGRLMYMGGNGFYWRIAHHPTRDGVIEVRRAEGGVRAWDALPGE
                     YHHSFTGEYGGLWRRNSRAPQKLVGVGFISQGFDKCSYYRRTDAAADPRAAWMFDGIE
                     DELIGDFGILQGGAAGLEIDAVDARLGTPPHALVVAQSENHSNTYEVVAEEVLIPHGA
                     TDAIINPEIHADITFFETPNGGAVWSTGSIAFAGSLGWNGFDNNVARLTTNVLRRFAA
                     EEPLPWPGRPEAGA"
     gene            10268..>11567
                     /locus_tag="P5558_15620"
     CDS             10268..>11567
                     /locus_tag="P5558_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25797.1"
                     /translation="MRHLLAGASALCLLGGMGIAQAAGEFDGVTVNVMTQTGAIQEPL
                     QRRAPEFEALTGAKINVIAVPFSDLYQKILTDWASGTNSVDAAVFAPQWMVDYIAGGY
                     LEDLTARVEADPAIEQDDVGAFFRDFSEKYAGSIYMITLDGDFHMMYYRKDLLEEAGL
                     EVPRTWDEYLEVAKALHGKDMNGDGTPDFGSCIAKKRNAQSYWFVMDVVGSMTQSKGT
                     SQGAFFNTADMTPLVDNEGFRKALDFLSESTKYGPPDELNLDVSDTRPLFASGRCALN
                     LDWGDVGTISIDPVNSKVIGKWGAAIMPGSTEVVNWETGELEACTADNCPYAIDGVNH
                     APFAAFGGWGGGINAAADDKVKDAAFAYFSYMTQPAQSNQDVTVGGTGFNPYRTSQLS
                     FSDLWKNAGMTEEEAAVYLGAINDSMNSPNMILDLRIPQNQ"
BASE COUNT         1855 a         3967 c         3662 g         2083 t
ORIGIN      
        1 ggtgacgacg gcggccgagg agaaggtcgc ggtcgcgacc cagctccagc tcacgtggtg
       61 gcggtttcgc cggcacaagg tggccgtggc cagcggcgtc atcgtgctct tcttctatct
      121 cgtcgcggtc ctcgcggact tcctcgccac caacgatccc tatgccacgc tcgccgcccg
      181 aagctacatc ccgccccagg aaatccactg gttcgacgcg gacggcagct tccgccccca
      241 cgtcttcgcg ctcgagggca agcgcgacat gaagaccttc cgtctcgtct atgtggccga
      301 ccccgaggac aagcgctacc tggagctctt cgtcgacggc tatccctacc gccttctcgg
      361 ccttttcgag accaacctgc atctgctcgg cctcgatcgc ggtgctcccg aggaagcgct
      421 cttcctcctc ggcaccgacc agctcggccg cgatttctgg tcccggctcg tgctggcgac
      481 ccggacctcg ctcttcatcg gcctcgccgg cgtcgccatc tcgctcttcc tcggcgtgct
      541 gctcggcggc atctcgggga tgtatggcgg gtggatcgac acggtgatcc agcgcgtcat
      601 cgagatcctc cgctcgatac ccacgattcc cttgtggatg ggcctcgccg cggcattgcc
      661 ggctacctgg agcgtgctcc aggtctatct ggcgatcacc gtgatcatct cgctgatcgg
      721 ctggaccgag ctcgcgcgcg tggtgcgtgg ccgtttcctc tcgctgcgcg aggaggattt
      781 cgtgatggct gccgagctcg ccggcgcctc gcagctgcgg atcatcttcc gccacatggt
      841 gccgtccttc ctgtcgcaca tcatcgccgc cgtcagcctc gccctgccgg cgatgatcat
      901 cagcgagacc acgctctcct tcctcggcct cggcctgcgg ccgccggcga tcagctgggg
      961 cgtgctgctg caggatgcgc agaacatcca ggccctggcc atcgccccct ggctgctcat
     1021 ggcggcgatt ccggtgatcg tcgtgatcct cgccttcaac ttcctcggcg acggcctcag
     1081 ggacgcagcc gatccctatg gctgagccga tcctctccgt ccgccggctc tgcacctggt
     1141 tccaccagga cgaggggatc gtgcgggccg tcgacggcgc gtccttcgac ctcgagcccg
     1201 gccatacgct cggcatcgtc ggcgaatcgg gctgcggcaa gagcgtcacc gcgcgctcga
     1261 tcctgcggat cgtcgagcgt cccggccgcg tcgaaagtgg cgaaattctg ctgcggcggc
     1321 cggacggctc ggtcgtcgac ctggtgaagc tcgatggcca gggccgggag atgcgcgagg
     1381 tccgcggcgg cgagatcggc tatgtcttcc aggagccgat gagctcgctc tcttccttcc
     1441 ataccatcgg caaccagttg atcgaggcca ttcgcctcca ttcggacatg agcaagaagg
     1501 cggcccggga tcgcgcggtc gagcttctga ccctggtcgg catcccgcgc cccgcccaac
     1561 gggtcgacag ctactcgttc gagctatcgg gcggcctgcg ccagcgggtg atgatcgccc
     1621 tggcactcgc cgccgagccg cgcatcctga tcgccgacga gcccaccacc gccctcgacg
     1681 tcaccaccca ggcccagatc ctcgacctcc tgcgcaagct gcaggccgag caggggctgg
     1741 cggtgatcct catcacccac gacatgggtg tgattgccga gatggcggac gatgtggtcg
     1801 tcatgtatct cggccgcgac gtcgagcagg ggccggtcga gacgatcttc cacgacccgg
     1861 cccaccccta tacccgctcg ctcctgcaat cgatcccgag cgtcctcgcc gagccgcgct
     1921 cgcgcctgcg caccatcgag ggctcgatcc cgcaccccta tgcccggccc gctggttgcc
     1981 ccttccaccc gcgttgcgcc gacgcgatcg ccggccgctg cgaccgcagc ctgcccgagc
     2041 ccacacacct cggcgcgcgg catgcggtga gctgcttcct ctatggcggg agcggggtcc
     2101 cggcactgcc tgcgggtctg ctggagcgtg ccgagtgacg gcggagccgc agacgatcct
     2161 cgaggtcgag ggcctcagga agtggtttcc gatcagccgc gggctgctcc gccgcgtggt
     2221 gggccatgtc cgggccgtcg atgacgtgac cttctccatc aagcatggcg agacgttgtc
     2281 gctggtcggc gagtccggct gcggcaagac cacgacgtcg cgctgcatcc tccgcgccct
     2341 gaccccgacc gacggggcga tccgctttcg aaccgagacg ggcgagcagg tcgacgtcgc
     2401 gaagctgccg aaggcggggc tccggccgct tcgtcggcag atgcagatga tcttccagga
     2461 cccgttctcg tccttgaacc cgcgcatgac catcaccgac atcatcgccg agcctttgct
     2521 cgtcaatggc gtgcgcgatc tcgagacgcg gcggcgccgg gtgcgcgagc tgctcgatct
     2581 cgtgcagctg cccgccgcct acatgaaccg atttccgcat gccttctccg gcggccagcg
     2641 ccagcggatc ggcatcgcta gagccctcgc cctcaacccg gcgctgatcg tcgccgacga
     2701 gccggtctcg gcgctcgacg tctcggtcca ggcccaggtc gtcaacctgc tgctcgacct
     2761 gcaggaccgg ctcggcctct cctatctctt cgtcgctcac gacctttccg tcgtgaagca
     2821 tgtgagcgac cgggtggccg tcatgtatgt cggcaagatc gtcgagctgg cacccacggc
     2881 cgacctcttc acccggcctc gccatcccta caccgaagca ctgctgtctg ccgtgccggt
     2941 gcccgatcct cggcgccgga ccgggcggat cgtgccgctg ggcgaggtcg ccgaccctgc
     3001 caacccgcct tcgggctgtc acttccatcc ccgctgccgc tatgctgtgg agcgctgccg
     3061 ggtcgagata ccggcgctgc ggcccatggc ggatgaccat ctcgcccgct gccatctggc
     3121 cgaggagctg aacttggctg gcgtgcagga gggccacccg ccagcaacga cgtgaaccct
     3181 ggagacccga gccatggcgg cgagcgcacc gcgtgttttc ttcgagccga agatcccgga
     3241 ctacgagccc ggactctatg acttcgagcg cttcgacgaa ggtgtaccgg ccacggccgc
     3301 gccggaagcg ctcgaccggt ttcgcgagga cggcttcctc atggtgcgcg gcctgctgcc
     3361 ggtcgaggag gtggtggccg cccgggccga gctcgaggcc atggcgctga gcgacgcgcc
     3421 cgactgctgg gggatctggt acgagggggc gctcagggac catatccgcc tcgatccgag
     3481 tctggatcgc gagatcgacg gcaaggtcgg cggcgtcggc ttcgcgccgg gccaggaggc
     3541 cgaccgcctg cccgatgtcg agcccgacct ccgcgcgcgc catgtgcgca agttcatggg
     3601 cttcttccgc cgccatccgg cgctgggtca gcttgcccgc catccggcga tcatggcgct
     3661 ggtcgaccgg ttgaccggcg ggtcgagcga gctcttccag gacatggcgc tggtcaagcc
     3721 ggcgcgcggc cgggagaagc cctggcacca ggatcacgcc tatttcaacg tggcaatcga
     3781 cacgccgatc gtcggcgtct ggatcccgat gggcgaggtg actccggaaa acggctgcat
     3841 gcacatgctg gccggcggcc acaaggaagg cccgcgcctc catttcaagc gtcgcgactg
     3901 gcagatctgc gataccgacg tggcgaccag cgggcgggtt gccgtgccga tgcggccggg
     3961 cgacgtgctg ctcttcgacg gcaagatccc gcatggcacg ccgaccaacc agaccgacag
     4021 cttccgctgg gccgtccagt accactatcg cccggccgat gctgtcttga tcgaggacgc
     4081 ggaacggctc gaggccttcg gcagcgaggg caagaacgtc acctgctgac gcggccagtt
     4141 ttccgcggca gcaccgttat ccggcattga cagccgccga taccgtcggc agcattcggg
     4201 ttgaggcgag acctatagcc tcagaaagat tttcttagcg caagtggccc ttgcttgcgt
     4261 acgctgcaac gacgacaacc ggtccgggag tagttggcat gcgtgacttg cccaatacaa
     4321 aatatgatct gagccgccgc cggctgctcg gctcggcggc cggtgccgcc acgctggctg
     4381 gcatgggcgg tctcgccaga tcgccactgg ccgcgacctg ggccgatcgg ccgccgagcg
     4441 aggtcgacct cctgaacttc gtcgtctgga cctatggcga catctacagc cagctcgcta
     4501 ccaggttcga ggaagactgg ggcgtgccgg tcgagtcggt gatctcgtcg ttcaacgacc
     4561 atccggccaa gctcatgacg atgttcgcga gcggcgacta catcgacgtc tcgcagtcct
     4621 cgcccttctc ctttcccaac ttcatccagc agggtctggt cgagccgctc gacggcctgc
     4681 cggggatcga ggagtatgtc gctgacttca gccccttcac caagcaggtc gccgtgcaga
     4741 acggccaggt catggggctg ccctatttct cggcgatctg ggtctggaac tactacgagg
     4801 acctgctcga gcaggctggc ctcgaaccct tccaggacta tgacgagctg attgagcagc
     4861 tgcgcaaggc gaagaaggac ggtgtcagcc agcacccgat cctctgggtc gccggcgtcg
     4921 gcctcgagca gttgccgggc acctggtact cactgacctg gaatcgtggc ggcacgttct
     4981 tcgagccgga cggcacgccg gccctggggc cgggctcagt ggcgcgcgag accctgcgct
     5041 ggtgggccaa gaccttcgag gaggagctgg ccgacccgga atcgctgaag gtccagttca
     5101 cgtcgtcggc caaggcgttc agcgccggca agaacctgta tcgcgggccc aaccaccact
     5161 atggtctgag catcgtcaac gaccctgagc agtcgccgat tgccggccgg gtcaaggtcc
     5221 acggcttccc cggtgacggg cggaccatcg gtgtcaccca tgtctacttc ctgtcgaccg
     5281 gcacccgtga ccgtgactgg gcatggaagc tgctgcagta tctcggcggc aggaccaagg
     5341 atggcgagta cacgcaggct gtcaatctcg ccaaggatgc catgctcggc agcggctacc
     5401 agtcggtgat ggaaagcgac atcatccgcg agagctgggc gcagtggggt gacgtcgacg
     5461 caatcctgga tatgtggggc aaggccacct acctgggtga ggtggtgagc tcggtcaacg
     5521 agccctggca cttcccctgg accgatcggc tcaatatcga ggtgcagaac tgcctgacca
     5581 ggcagatcga tgccgatcag tgctgcgata tcctgacggc cgcggtcgac gaggcgaagc
     5641 gcggctgagc ccggttcgcg gcagaggtcg gatattggca gcggacgcag aaagacggaa
     5701 caccgacgcg gccagcgggg ccgcgtcggg gcgggaactc tcgccgcgtt cgctcgcggt
     5761 ggtcatgaac gtcccgacaa tcctcatcct ggccctggtg ctggcctatc cgatctacta
     5821 cgcgggtttc ctctccttcc accaggtagg tccgggccag ctacgcagcg gcgtcttccc
     5881 gtttgttggt ctgctcaact acgagcgcgt cctgaccgat ccgctgttcc agaaggcgct
     5941 tctcaacacc ttcaccttca gcgccatcgt cgtcgtcgcc gagatcgtgc tggcggtggc
     6001 gatcgccctc ctgatcaacc agcagggcat ctggacatcg cgggtcaccc gcatcctgat
     6061 gctcgttccc tacgcggtgc cgccgatcgc caacggcctc gtctggtcgt tcatgtacag
     6121 ctaccagttc ggcttcctga accgcatcct cttcaccctg ggagtgatca ccgagccgat
     6181 caactgggcc ggcaacccgg acacggcact cttcgccatc actgttccct atgtctggcg
     6241 cacgttgcct ttcgccattc tgctcgtcca tgccgcgttg cagggcatca acaaggagct
     6301 gcacgaggcg gcggcagtcg acggcgccaa tgcctggcgc cggttctggc acatcaccct
     6361 gccgctcctg acgccgatca tcgtcgtcat cctcgtgctg cgcacgtcgt tcgccttcgc
     6421 cgtcttcgag gagatcctgg cgatcaccca gggcggcccc ggcgatgcga cctgggtggc
     6481 tgcctggtac agctaccgca agtccttcgc gccgccgttc gacatcggtc tcggctcggc
     6541 ctccgcctat ctcctggcga tgctggtcgg catcctggcg ctcgtctatg tccgcatgat
     6601 ctaccggcgg gtcgagtagc gtcgtgctca agccatcgcc gatcgcccag atcctcctgc
     6661 atttcgccaa tgtactggtg atcgccttct tcctgctgcc gatcgtggcc gtcaccatcg
     6721 gctcgctgca atcggagaag atgctgcagg ccgacaccca ggcgatcctg ccggccgagt
     6781 ggaccttcga caatttcctc gtcatcctct ccggcggcga gcagcgcggc aagatcttcg
     6841 agcaggtgac ctacctgccg gacaacgtga agagctttca ccgcgccttt gccaacagcg
     6901 tgatcgtggc gacttcggtc accattttga cgctcgtctt cggctcgctc tcggcctaca
     6961 cggtggcccg gctgcgcttc ggctggacga tctggctgat gaacctcaac gtcatggcgc
     7021 gcttcgtgcc gatcatcgtc ctgatgattc cgctctacgt gacctttcgc aatttcggca
     7081 tgctgaactc gctgagcggc gtgatcgtgg cgcttacagg gttcctcctg ccttatgcga
     7141 tcctgatgct ggcgccgtat ttcgccacca tcccgcgcga gctcgaggat gcggcgcgca
     7201 tcgacggctg cacgcatttc agcgcattct ggcgcatcac cttgccgctc tcggcgccgg
     7261 ggctcgcctc gtgcggggcg atcatgttca tcatctcctg gcacgagctg ctgatcccct
     7321 tgatcctcaa ttcgcgcgcc gagttcatga cactgcccgt ggtcatcgcc agcctggtcg
     7381 gcgacgtgca tgtcttcttc aacctgatga tggcgatctg cctcctggcc atgctgccat
     7441 cggtcattct cgtcctgttg ctgcaaagat tcgtcgtcca gggcctgcag gtcggcggcg
     7501 tcaagggttg aacgatgtac agcttcgaca agtctcgcct cgacctcgcc cgggagttca
     7561 aggccaatcc gttcggcgag catcgcccgg acctgcaata tctcctgaac ctgatgcgcc
     7621 ggcccgaccc gaagcgcccc cacttcgtcc tgatgccccg gcaatggggc gaatcctgga
     7681 cgctggcggt gatggagccc ggcggcacaa gcccgccgcg cccgaccaac ctcgtcttca
     7741 ccgatgcagg cgaggcggaa tggcatgtct tcaagctgcg ctgggccgag ctcgccggcg
     7801 aagagctgcc gttcgactga cgaagcgttg gagaagcctg ttctcaagac ggtgttgagc
     7861 cgctcgctgg actgaagaga tcaatgactc gaatcatggg ctactgcgat cgttggagcg
     7921 tcaggccggg cgacaccgtg aacttcatgg tgagctgcat tggcgccgag agctacgcgg
     7981 ccgagatcgt ccgactgaag caacccgagg ctggcccgct cgccacgccg ttcgagccgg
     8041 agcctgttgc ctcggacgtc aacggccgct atgcgggccg cgagcaggcc ctgccgatcg
     8101 gctcgctcgc cgtggtgact ggccacccgt gcctggagcg gctcgaggcc gccacgctcg
     8161 tggcctacgt catgccgacc acgccctcga agggccgcca ggcgctcttc ggcaactggt
     8221 gcgaagcgag ccgcaccggc ttcgggctcg agctcgacga gcagggcagt ctcgtgctgc
     8281 gcctcggcgc gggccgtggc accgaggtga cgctcgctac tggcgagccg atgcgtgcct
     8341 ggcgctggta tcgtgtggcc ggcacctttg acagccagag tggccgcatg gcgatctggc
     8401 aggaaccggt ctcccgccat ggcttcgatc tcgatcgacc gatcgcacgc agcgccgaac
     8461 gcgggtgccc gagcacccgt tcgatgaacc cgctcacctt cgctgcctgg cagaatggcg
     8521 agacagcggg gccctcggcc tggggcggtc tgcagttcgc ccagcacttc aatggccgca
     8581 tcgaccggcc gcgcatcgtc gagggtgctc tcgaccacgc cggcatcgcc gagctgatgg
     8641 gcgagacggg gcctgccgcc atcgagcacg agctcgtcgc ggcctgggac ttctccctgg
     8701 acatcggcgg cgaggcgatc atcgacaccg ggccgaaccg cctgcatggc accatcgtca
     8761 acctgccgac ccgggccgtc cacgggcaca actggaccgg ctgcatcaac gactggaccc
     8821 aggcacccga gcagtatggc gccatccatt tccacgacga cgatatcgtc gacgcctgct
     8881 ggacggtgga cttcagcttc gaggtcactt cagcacagcg ttcgggcgtc tatgcggcga
     8941 agctgactgc cggcgacttc gtcttctggg tgccgttctt cgtcagcccg cccaagggcg
     9001 aggcgcgctc gaaggtcgcg ttcatcgcgt cgacggcaac ctacaccgtt tatctcaaca
     9061 acaagggacg ctttctctcg agcgccaccg aggtctacca gggccgggtg acggtcatgg
     9121 acgagatcga ttccctcttg ttcgagtacc cggcgatggg tctctcgacc tatgaccggc
     9181 acagcgacgg caccggcgtc gcctatgcca gccggcatcg cccggtgcac aatttccgcc
     9241 cgaccggccg ccattggaac ttcaatctcg acctctgcat catcgattgg ctcgagcatc
     9301 tgggcggcga ctacgacgtc atcaccgagg agcagctgca cgaggagggc atgggcctcc
     9361 tcgcacccta caacgtggtg atcaccggct cccatcccga gtacgactcg ctcgacatgc
     9421 tcgacgcctt ccaggcctat ctgcgccgtg gcggccggct gatgtacatg ggcggcaacg
     9481 gcttctactg gcgcattgcc caccacccga cgcgcgacgg ggtgatcgag gtgcgccggg
     9541 ccgaaggcgg tgtgcgtgcc tgggatgccc tgccgggcga gtaccatcac agcttcaccg
     9601 gcgagtatgg cggcctctgg cggcgcaatt cgcgggcacc gcagaagttg gtcggtgtcg
     9661 gcttcatcag ccagggcttc gacaagtgca gctactaccg tcgcaccgac gccgccgccg
     9721 acccccgggc ggcttggatg ttcgacggca tcgaggatga gctgatcggc gatttcggca
     9781 tcctgcaggg cggtgccgcc gggctcgaga tcgatgccgt cgatgcgcgg ctcggcaccc
     9841 cgccgcacgc gctggtcgtg gcccaaagcg agaaccactc caacacctac gaggtggtcg
     9901 ccgaggaggt gctcattccg cacggcgcca cggatgccat catcaacccc gagatccacg
     9961 ccgacatcac cttcttcgag acgcccaacg gcggggccgt ctggtcgacc ggctcgattg
    10021 cctttgccgg ctcactgggc tggaacggct tcgacaacaa tgtcgcccga ctgacgacca
    10081 acgtgcttcg ccgcttcgct gcagaagagc cgttgccctg gcccggtagg ccagaggccg
    10141 gggcataggt caggcagcca tggtgattgc tgccgttcgg gcgaatggct agtgttcgac
    10201 cagggtcgga gcaatgggcc gaaaagccgt tggcgaccgc ggatcaagaa aaggggaggt
    10261 agacatcatg cgacatctgc tggccggcgc ctcggcgctt tgtctgctcg gcggcatggg
    10321 cattgcccag gcagcgggcg agttcgacgg ggtcaccgtc aatgtcatga cccagaccgg
    10381 tgccatccag gagccgctgc agcggcgggc accggagttc gaggcgctca ccggcgcgaa
    10441 gatcaacgtc atcgccgtgc cgttctcgga tctctaccag aagatcctga ccgactgggc
    10501 gagcggcacc aactccgtcg acgccgcggt tttcgccccg cagtggatgg tcgactacat
    10561 cgccggcggc tatctcgagg acctgaccgc gcgggtagag gccgatccgg cgatcgagca
    10621 ggacgacgtg ggtgcctttt tccgcgattt ctcggagaag tacgccggca gcatctacat
    10681 gatcacgctc gatggcgatt ttcacatgat gtactacaga aaggacctgc tcgaggaggc
    10741 ggggctcgag gtgccgcgca catgggacga gtatctcgag gtcgcgaagg ccctgcacgg
    10801 caaggacatg aacggcgatg gcacccccga tttcggctcg tgcatcgcca agaagcgcaa
    10861 tgcccagagc tattggttcg tgatggacgt cgtcggctcg atgacccagt ccaaggggac
    10921 gagccagggc gcgttcttca acacggccga catgacgcct ctggtggata acgagggctt
    10981 ccgcaaggcg ctcgatttcc tcagcgaatc gaccaagtac ggcccgccgg acgagttgaa
    11041 cctcgacgtc tccgacaccc gccccctttt cgcttccggc cgttgcgcgc tcaatctcga
    11101 ttggggtgac gtcggcacga tctcgatcga cccggtgaac tccaaggtca tcggcaagtg
    11161 gggtgcggcc atcatgccgg gctcgacgga agtggtgaac tgggagaccg gcgagctcga
    11221 ggcctgcacg gcagacaact gcccctatgc catcgacggc gtgaaccatg cgccgttcgc
    11281 ggccttcggc ggctggggcg gcggcatcaa cgccgcagcc gacgacaagg tgaaggacgc
    11341 ggctttcgcg tatttctcgt acatgacgca gccggcccag tcgaaccagg acgtcaccgt
    11401 cggcggcacc gggttcaacc cctatcgcac ctcgcagctc tccttcagcg atctgtggaa
    11461 gaacgccggc atgaccgaag aggaggccgc ggtctatctc ggcgccatca acgacagcat
    11521 gaacagcccg aacatgatcc tcgatctgcg catcccgcag aaccaga
//
LOCUS       DAOTFI010000157        11561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6638_length_11561_cov_6.860247, whole genome shotgun sequence.
ACCESSION   DAOTFI010000157 DAOTFI010000000
VERSION     DAOTFI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11561
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6638_length_11561_cov_6.860247"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1265)
                     /locus_tag="P5558_15625"
     CDS             complement(138..1265)
                     /locus_tag="P5558_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRY25798.1"
                     /translation="MTTRLPPFGGASDRRNSMADKMKAAIFIEPGRIVLGEKPMPQIG
                     PNDALIRITTTTICGTDIHILKGEYPVEKGLTIGHEPVGVIEKLGRNVVGYTEGQRVI
                     AGAICPSGHSNACLDGLHSQDGPGTAHGLKPMGGWRFGNTIDGAQAEYLLVPDAMANL
                     AAVPDGLTDTQVLMCPDIMSTGFAGAEAANIRIGDTVAVFAQGPIGLCATAGARLRGA
                     TTIIAVDSVPERLAMAKRLGADAVVNFKEVDAVEAIMELTNGRGVDAAIEALGLQQTF
                     ASCLEVLKPGGTLSSLGVYSSDLTIPLAAFHAGLGDNKIVTSLCPGGKERMRRLMNVI
                     AAERLDLGPLVTHHYGLDRIEEAYELFANQRDGVLKVAITT"
     gene            1374..2312
                     /locus_tag="P5558_15630"
     CDS             1374..2312
                     /locus_tag="P5558_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25799.1"
                     /translation="MRPGLWTATILSAALAPLVAAAQSHPVTFGMQTAMETFFGEDGI
                     VSFGEYDAAFVPPDGLVAEVALADEAGNVLGRFPFHDSYRFQDKVFARIGVVGPADVQ
                     LDTPGVYNIFFLVDGEIATRLPFVLKAASDGSDPFDPEKTWTVDGFWRQLGYMTELNS
                     SAGPAPRFTFWTGGPDLPEGATGDGYVASLVRDGEVIAHSKRTTGHIPPGRFARTTFE
                     LFHPHGEKETPNAEIVTLADLAAQPDGELLLRIFRSSDNALIRVFHLNMAGGTLQQHE
                     RAVLGYEPQMDFILPRVVRAGSNTYELVEATWFESF"
     gene            2954..3958
                     /locus_tag="P5558_15635"
     CDS             2954..3958
                     /locus_tag="P5558_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY25800.1"
                     /translation="MDIGSRSRSTAQDVAERAGVSRAAVSRCFNARGRVSGDKRERIL
                     AAASELGYRPNAIARSLTGQPTDLVALITTESLGYQSNEQLRAITLCLAERGKRALII
                     PVGAKTTLDESSLRALDYQVDAIVVMGGSISRHVVELLAAARVPLLLFGRAHEGAGTA
                     AICCDNAEGGAIAARCLTRLGRTRLAYVGKSAGTFADRARLGGFVGELRRQGLEPCDM
                     AADESSFAGGRRAAATLLAAAEPPDGLFCFNDTVAFGALQAARDFRLRVPEDVAIVGF
                     DDQPMAAWPAFDLTTVGCDSGALAELAAAKILAALGRGEQVEGTFLVRPYLVVRGTTP
                     "
     gene            3955..5793
                     /locus_tag="P5558_15640"
     CDS             3955..5793
                     /locus_tag="P5558_15640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY25801.1"
                     /translation="MTGGNFTAAALARSGALRSADWRYRLAVGWREPTAAIGVLLALL
                     FGYLIVVPIVTLLHDAIEVQFGDARRAGAEVGGWTLHYLERAMFSPVAGDLFWEPLRH
                     TLAISAGAISFSLVVGGLLAWLLTRTDMLGRRWFATALIVPYMLPSWTFALAWRTIFK
                     NRAVGGQPGWLESLGFTPPDWLAYGQVPITLILALHYTPFVILLFGNALRRFDSQLED
                     SARMLGAGPATVARRIVLPMMLPALMSATLLIFAKCLGEFGVSYVLGLPVNYDVLATS
                     LYRSIASRQSGVAAVIAAAIMLIGVLSLLIDARLVRQARRFVTVGGKGAMNRRSGLGI
                     LGLPALALASFIFAISVALPLLTLVLSTIMRVPGDFSLANFTLDFWIGSNLETVALRS
                     GLLLTPELWLAAWNTLRIVGIASICSGLCGLLVGYVVVRSPVRPMAMFLRQVTFFPYL
                     VPGIAFAAAYLSLFAVPRGPIPALYGTAIILILALAADQMPFASRAGISAMMQLGKEP
                     EEAAQIIGAGWLRRIVSVVVPIQKGALVAGILLPFISGIRGLSLFVILAVPSTDVLTT
                     FSLRLLDYNYTQAANGVVLLICAISYVGTLAAQKLTRTNLAEGLGG"
     gene            5795..6961
                     /locus_tag="P5558_15645"
     CDS             5795..6961
                     /locus_tag="P5558_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25802.1"
                     /translation="MPDIVLRQVNKRYDRDGPDAVADLDLTIGDGEFMCLLGPSGCGK
                     TTTLRLIAGLENLSGGEIAVGDEIVDSRSTGTFVPPERRGMGLVFQSYALWPHLTIEE
                     NVDFGLRLRRMPKPERRERVTRVMETLGIEPYRDRYPSQLSGGQQQRVALARMLAVEP
                     RVLLLDEPLSNLDARLRLEMRAELKRIHSAFGTTIVFVTHDQWEAMTLATSIAVMHQG
                     TLQQVGEPTDIYDRPANRFVAEFVGSPPINIVEVEGPETSPLVALARAFLETTRQGRG
                     EIASIGIRPEHLHLLDPNEPVAGGAFTGPATLSALLPTGGSWIIELDVGGSPLFMSTN
                     RPPVFRVGDSITFSVERDAVHHFDRNLSRATTTGAPKEKVSAGADTDTSPRAAK"
     gene            6996..8114
                     /locus_tag="P5558_15650"
     CDS             6996..8114
                     /locus_tag="P5558_15650"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25803.1"
                     /translation="MARPIHHVQRCATALVLVGLAAPAMAQDFDLDALIEAAKAEGPI
                     TIYASTGKIKTSAEAFTAAYGIEAIGTKVDGATQIELVLREAQAGNIVGDVIISSDAA
                     STLAQLLPEGIVTSWTPPDLAGTIPEDAQDPLVVWRDPAVWTYNGEKYETCPVDNIWA
                     LTEPEWNRRVAMPDPLNKPSFVDWFNQMETHWDATIAAAYEAHFGQKLDTSNESATAA
                     WVQALAENAPLVTDSDSAAAEAIGTPGQDEPFFGILATAKYRDNADGGLHLALCEGIE
                     PFIGFANPSYGLIANGTASPNAAKLFLRFMMTAEGVEPMTVDGKVSGNEAVPSHPDEP
                     SGVAAFASRLTPHLAATGGEDFDRRQDWQDFWRIHYAR"
     gene            8220..8852
                     /locus_tag="P5558_15655"
     CDS             8220..8852
                     /locus_tag="P5558_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25804.1"
                     /translation="MAPIFQRPALSLISEGHGPDRASLFIGGTEAARDLAALDAAGIS
                     TVVNCAVNLDINYVRDPAFAAAPGKCAAGPGAVRSYKIGLIDGDGNPRQMLLAGYYIL
                     DGALTQTMPDKASYPHRTRGHVLVHCRGGRSRSVALVALYLHCQEPERYPRLDDAIAH
                     VRRRRELREEEWHETPKPVLVEAARYAAAAIRLLDETSLHAPAEPECRPA"
     gene            complement(8866..9177)
                     /locus_tag="P5558_15660"
     CDS             complement(8866..9177)
                     /locus_tag="P5558_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRY25805.1"
                     /translation="MIYDMRIYTCRPGTVPAQLALYKEHGYATQTKHLGQPVLYGVTE
                     TGPINTYVHVWAYESAADREQRRAAMTADPAWTAFLTKSREAGNLIAQENRLLTGVSF
                     G"
     gene            9322..10314
                     /locus_tag="P5558_15665"
     CDS             9322..10314
                     /locus_tag="P5558_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HRY25806.1"
                     /translation="MTSLTRRGLMAATAIAGVGIAAPTVLRAQGTVIRWGEMLPATHP
                     QALMIDRIAATVAEQSDGRIEIQSFPNGQLGSGKDMMDSVVSGALAMTTDGAAALGSL
                     LPQLSVIEAPYLWRDAAHMAKVADAPVFAKMNDDLVAQRGLKMLAVTYYGKRHLTTGT
                     REVHTVADMEGFKLRVPPVDTFRAMAEAWGAQATPINFTELYLALSQGAVDGQENPLP
                     TIASAKLNEVQKYLVLTGHIITPRLVIANNDWLNGLEEADREILEGAIAEAVAWQDAE
                     LASQEASLQGTLEQQGMIVIEPDVESFRKPVLEQLPPKFVDKWGEGTWDALAAL"
     gene            10342..10815
                     /locus_tag="P5558_15670"
     CDS             10342..10815
                     /locus_tag="P5558_15670"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRY25807.1"
                     /translation="MLPSLARVAQRLLEVAAVLLLLAMLASVFLGVVFRLANQPLTWS
                     DEMATYLLVWTGFVGWIIAGQRGSHIRITAIIGRLRGTPLMLAEVVIQLLVLVFGLIL
                     LTQSFGLIQRNLDVEWVSLPLSVALVYIPIPIAGFAVVLQAGIEILRLVRERAVS"
     gene            10812..>11561
                     /locus_tag="P5558_15675"
     CDS             10812..>11561
                     /locus_tag="P5558_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY25808.1"
                     /translation="MTAIMVEILIVWFVALMLGVPLFATLGLASAAFVWLNGMPIDIV
                     PQKLAQSVNSFPLLAAPLFILMGAIMNSAGITDRIFGFATACVGWVRGGLCHANILAS
                     VIFAGMSGSAVADAGGVGALEIRAMKQAGYDAETAAAITAASATIGPIIPPSLPMVIY
                     GVSADVSIGGLFLAGVVPGLLMAGALSAMVVVMARRRNMARELFPGLRGLWLAYRRAH
                     WALMTPVILFGGMMAGIMTPTEAAAVATVYAL"
BASE COUNT         1642 a         4033 c         3973 g         1913 t
ORIGIN      
        1 ccgaccaccg cctggtcctc gccggcatga acccgatcgt ccgcgaggtc ttcaacgtct
       61 ccggcttcga ccgcatgctg gtcatcgagc atgatctggc gatggcggtc gccgcccaag
      121 gctgagggcc gggtcgctca ggtggtgatg gccaccttga gcacgccgtc gcgctggttg
      181 gcgaagagct cgtaggcttc ctcgatccgg tcgagcccgt agtgatgcgt gaccaagggg
      241 ccgaggtcga ggcgctcggc ggcgatgacg ttcatcagcc ggcgcatgcg ctccttgccg
      301 ccggggcaga gcgaggtgac gatcttgttg tcgccgaggc cggcatggaa ggcggcgagc
      361 gggatggtga gatcgctgct gtagacgccg aggctggaga gcgtgccgcc gggcttgagc
      421 acctcgaggc agctcgcgaa ggtctgctgc aggccgagcg cctcgatcgc cgcgtcgacg
      481 ccgcggccgt tcgtcagttc catgatcgcc tccaccgcgt cgacctcctt gaagttgacg
      541 acggcgtcgg caccgagccg cttcgccatg gcgaggcgtt ccggcaccga atcgacggcg
      601 atgatggtcg tcgcacccct gagcctcgcc cccgccgtgg cgcagaggcc gatcgggccc
      661 tgcgcgaaga ccgccacggt gtcaccgatc ctgatattgg cggcctccgc accggcgaag
      721 ccggtcgaca tgatgtccgg gcacatcagc acctgcgtgt cggtcaggcc gtccggcacg
      781 gcggcgaggt tggccatggc gtccggcacc aggaggtatt cggcctgcgc accgtcgatg
      841 gtgttgccga agcgccagcc gcccatgggc ttgagcccgt gcgctgtgcc cggcccgtcc
      901 tgcgaatgca gcccgtcgag gcaggcgttg ctgtgccccg acgggcagat cgcaccggcg
      961 atcacccgct ggccctcggt gtagccgacg acgttgcggc cgagcttctc gatcacgccc
     1021 accggctcgt ggccgatggt caggcccttc tcgaccgggt actcgccctt gaggatgtgg
     1081 atgtccgtgc cgcagatggt ggtcgtcgtg atgcggatca gggcgtcgtt cgggccgatc
     1141 tggggcatcg gcttctcgcc caggacgatg cgtccgggct cgatgaagat cgccgctttc
     1201 atcttgtcgg ccatggagtt cctcctgtcg ctggcgccgc cgaagggcgg cagacgggtc
     1261 gtcatgatgt aaccggatat tcgccgtggc cgccttgcgc tggatcaagg ggcctaccgg
     1321 ttcaggatcg gcgccgatag gatgccggtc gacgactggc agggagaacc gctatgcgac
     1381 caggcctctg gacggcgacg atcctctccg cggcgctggc cccgctcgtg gcggcggcgc
     1441 agagccatcc cgtcaccttc ggcatgcaga cggcgatgga gaccttcttc ggcgaggacg
     1501 gcatcgtcag cttcggcgag tacgacgcgg cgttcgtccc gccggacggg ctcgtggccg
     1561 aggtggcgct ggccgacgag gcgggcaacg tgctcggccg ctttcccttc cacgacagct
     1621 atcgcttcca ggacaaggtc ttcgcccgga tcggcgtggt cgggccggcc gacgtgcagc
     1681 tcgacacgcc cggcgtctac aacatcttct tcctcgtcga cggcgagatc gcgacccgcc
     1741 tgcccttcgt cctgaaggcg gcgagcgacg gcagcgaccc gttcgacccc gagaagacct
     1801 ggacggtcga cggcttctgg cggcagctcg gctacatgac cgagctcaat tcgagcgccg
     1861 gcccggcgcc gcgcttcacc ttctggaccg gcgggccgga cctgcccgaa ggggccacgg
     1921 gcgacggcta tgtggcgtcg ctggtcaggg acggcgaggt gatcgcccac agcaagcgca
     1981 ccacgggcca catcccgccc ggccgcttcg cccgcaccac gttcgagctc ttccacccgc
     2041 acggcgagaa ggagacgccg aatgcggaga tcgtcaccct cgccgacctg gcggcccagc
     2101 ccgacggcga gctgctgctg cgcatcttcc gcagctccga caacgcgctc atccgcgtct
     2161 tccacctgaa catggccggc ggcaccctgc agcagcacga gcgcgccgtg ctcggctacg
     2221 agccgcaaat ggacttcatc ctcccccgcg tggtccgcgc cggctcgaac acctacgagc
     2281 tggtcgaggc cacctggttc gagagcttct gaccgattta ggaataatag cctgaatcgc
     2341 gcgccacgtc aaggctcgcg ggttcgttgg gcaattcgcg cttttcgggt tcggtcgtcg
     2401 gcgacatgcg gtccaaatgc ccgggatcac tggtccgggc cctcgggttc gttcgtcgaa
     2461 tctctgggtt cgttcgtcaa aaacccgggt tcgatcgtcg attccccggg tttgttcgtc
     2521 gattccgcgg gttcgttcgt cgcaggatgt agcgccagac gcgacatccc ggggattctg
     2581 ttgcagattc cgcgacattc cggggctgcg tgtcgcggta tatgcgccaa cccgagcgat
     2641 tttgttgcag attccgcgac aacccgagcg gcgatgtcgc acgagatgca cccccggcga
     2701 gggggaggcg gtcgcagcgg cgtgcatggg aagggcgtgc cgcgcaagcg tcacctctcc
     2761 agagatgaac gcgcgttcac attggtgtcg aaagacctgt gacagggtgt cgggtcggcg
     2821 acggcgagcg gcttcggcgc cactcctgct ccaggcgagc tccgaaccgg cgctcggccg
     2881 cgccggtcgc gctcccgttg acaccccgtg ccgtgccgtg tgaattggac gccggcattg
     2941 tgatcgcgcg atcatggaca tcggctctcg atctcgaagc acggcgcagg acgtggcgga
     3001 gcgggcaggc gtctcgcggg cggctgtctc gcgctgcttc aacgctcgcg gcagggtctc
     3061 gggggacaag cgggagcgca tcctcgccgc ggccagcgag ctcggctatc gccccaacgc
     3121 catcgcccgc tcgctgaccg gccagccgac ggacctcgtc gccctgatca ccacggaaag
     3181 cctcggctac cagagcaacg aacagttgcg tgccatcacc ctgtgcctcg cggagcgcgg
     3241 caagcgggcg ctgatcattc ccgtcggcgc caagaccacg ctcgacgaat cgtcgctgcg
     3301 ggcgctcgac taccaggtcg atgcgatcgt cgtcatgggt ggcagcatct cgcggcacgt
     3361 ggtggagctg ctcgccgccg cgcgggtgcc gctccttctc ttcggccggg cgcacgaggg
     3421 cgccggcacg gccgccatct gctgcgacaa tgccgaaggc ggcgccatcg ccgcgcgctg
     3481 cctgacgcgc ctcggccgca cccgcctcgc ctatgtcggc aagagcgccg ggaccttcgc
     3541 cgatcgcgcg cgcctgggcg ggttcgtggg cgagcttcga cggcagggcc tcgagccctg
     3601 cgacatggcg gccgacgaga gcagctttgc gggtggccgt cgcgccgccg ccaccctgct
     3661 cgccgccgcc gagcctcccg acgggctctt ctgcttcaac gacaccgtgg ccttcggcgc
     3721 cttgcaggcg gcccgcgact ttcgccttcg cgtgccggag gatgtcgcca tcgtcggctt
     3781 cgacgatcag cccatggcgg cctggccggc cttcgatctg accacggtcg gttgcgattc
     3841 gggcgcgctg gccgagctcg ccgcggcgaa gatcctggcg gccctgggcc ggggagagca
     3901 ggtcgagggc acctttctcg ttcgccccta cctggtcgtc aggggcacca cgccatgacc
     3961 ggcggcaact tcacggcggc cgctctcgcg cgatccggcg ccctgcgctc ggccgattgg
     4021 cgctatcgcc tcgccgtggg ttggcgcgag ccgacggccg ccatcggtgt gctgctggcg
     4081 ctgctcttcg gctatctgat cgtcgtgccg atcgtcacgc tgctgcacga cgcgatcgag
     4141 gtccagttcg gcgacgcgcg gcgggccggg gccgaggtcg gcggctggac gctgcactat
     4201 ctcgagcggg cgatgttctc gccggtggcg ggcgacctct tctgggagcc gctgcgccac
     4261 acgctcgcca tctccgctgg cgccatctcg ttctcgctcg tggtcggcgg cctgctcgcc
     4321 tggctgctca cccgcacgga catgctgggc cggcgctggt tcgccaccgc gctcatcgtg
     4381 ccctacatgc tgccgtcctg gaccttcgcg ctggcctggc ggacgatctt caagaaccgg
     4441 gcggtcggtg gccagccggg ctggctcgag agcctgggct tcacgccgcc cgactggctc
     4501 gcctatgggc aggtgccgat cacgctcatc ctcgccctgc actacacccc cttcgtcatc
     4561 ctcctcttcg gcaacgcgct ccgccggttc gacagccagc tcgaggattc ggcgcgcatg
     4621 ctcggcgccg ggccggcgac ggtggcgcgg cgaatcgtgc tgccgatgat gctgccggcc
     4681 ctgatgtcgg cgacgctgct gatcttcgcc aagtgcctcg gcgagttcgg cgtcagctac
     4741 gtgctcggcc tgcccgtgaa ctacgacgtg ctggcgacgt cgctctatcg cagcatcgcg
     4801 tcccgccaga gcggggtggc cgcggtgatc gcggcggcga tcatgctcat cggcgtcctc
     4861 tcgctgctga tcgacgcgcg cctggtgcgc caggcccgcc ggttcgtcac ggtgggcggc
     4921 aagggggcga tgaaccgccg gtccgggctc ggcatcctcg gcctgccggc gctggcgctg
     4981 gcgtcgttca tcttcgccat cagcgtcgcc ctgccgctcc tgaccctggt cctctcgacg
     5041 atcatgcggg tgcccggcga tttctcgctc gccaacttca cgctcgactt ctggatcggc
     5101 agcaacctcg agacggtcgc cctgcgctcg ggcctgctgc tcacgccgga gctgtggctg
     5161 gcggcatgga acaccctgcg catcgtcggc atcgcctcga tctgctccgg gctctgcggg
     5221 ctgctcgtcg gctatgtcgt ggtgcgctcg cccgtgcggc cgatggccat gttcctgcgc
     5281 caggtcacgt tctttcccta tctggtgccg ggcatcgcct tcgccgccgc ctatctctcc
     5341 ctcttcgcgg tgccccgcgg cccgatcccg gcgctctacg gcacggcgat catcctgatc
     5401 ctggccctgg ccgccgacca gatgccgttc gcctcgcgcg ccggcatctc cgccatgatg
     5461 cagctcggca aggagccgga ggaagcggcc cagatcatcg gcgccggctg gctgcggcgc
     5521 atcgtttcgg tggtcgttcc catccagaag ggcgccctcg tcgccggcat cctgctgccc
     5581 ttcatctccg ggatccgcgg cctgagcctg ttcgtcatcc tggccgtgcc gagcaccgac
     5641 gtgctgacca ccttttcctt gcgtctcttg gactacaact acacgcaggc ggccaacggc
     5701 gtggtcctgc tcatctgcgc catctcctat gtcggcacgc tggcggcgca gaagctcacg
     5761 cgcaccaacc tcgccgaagg cctgggaggc tgaagtgccc gacatcgtcc tgcgccaagt
     5821 caacaagcgc tacgaccggg acgggcccga cgccgtggcc gatctcgacc tcaccattgg
     5881 cgacggcgag ttcatgtgcc tgctcgggcc gtcgggctgt ggcaagacga cgacgctgcg
     5941 cctgatcgcg gggctggaga acctgtccgg cggcgagatc gcggtcggcg acgagatcgt
     6001 cgattcgcgc agcaccggca cgttcgtgcc gcccgagcgg cgcggcatgg ggctcgtctt
     6061 ccagagctat gcgctctggc cgcatctgac catcgaggag aacgtcgatt tcggcctgcg
     6121 gctgcgccgg atgccgaagc ctgaacggcg ggagcgggtc acgcgggtca tggagacgct
     6181 gggcatcgag ccctatcgcg accgctaccc gtcccagctc tcgggcggcc agcagcagcg
     6241 cgtcgccctg gcccggatgc tcgccgtcga gccgcgggtc ctgctcctcg acgagccgct
     6301 ctccaatctc gatgcccgcc tgcgcctcga gatgcgcgcc gagctcaagc gcatccacag
     6361 cgccttcggc acgaccatcg tcttcgtgac gcacgaccag tgggaggcga tgaccctcgc
     6421 caccagcatc gccgtcatgc accagggaac cctccagcag gtaggcgagc cgaccgacat
     6481 ctacgaccgg cccgccaatc gcttcgtcgc cgagttcgtc ggcagcccgc cgatcaacat
     6541 cgtcgaggtc gaggggccgg agacgtcgcc gctggtggcg ctcgcccgtg cgtttctcga
     6601 aacgacccgg caggggcgag gcgaaatcgc ctcgatcggc atccgccccg agcatctcca
     6661 cctgctcgac ccgaacgagc cggtcgccgg cggtgcgttc accggcccgg ccacgctctc
     6721 ggcgctgctg ccgacgggcg gcagctggat catcgagctc gatgtcggcg gctcgccctt
     6781 gttcatgtcg accaaccggc cgcccgtctt cagggtgggc gattcgatca cgttcagtgt
     6841 cgagcgcgac gccgtgcacc atttcgatcg aaacctcagc cgcgcgacga cgacaggcgc
     6901 accgaaggag aaggtgtcgg caggtgccga caccgatacg tcgccccggg ccgcgaagtg
     6961 agcccgggcg ttcaaccaac caggagagag atcagatggc tcgaccaatc caccacgtgc
     7021 agagatgcgc cacggcgctc gtcctcgtcg gcctcgccgc accggcgatg gcccaggact
     7081 tcgacctcga cgcgctgatc gaggcggcga aggcggaggg cccgatcacg atctatgcca
     7141 gcaccggcaa gatcaagacc tcggcggagg cgttcaccgc ggcctacggc atcgaggcca
     7201 tcggcaccaa ggtggacggc gccacccaga tcgagctggt gctgcgcgag gcccaggccg
     7261 gcaatatcgt cggcgacgtg atcatctcga gcgacgccgc ctcgaccctg gcgcagctcc
     7321 tgccggaagg gatcgtcacg agctggaccc cgcccgacct cgccggcacg atccccgagg
     7381 atgcccaaga tcccctggtg gtctggcggg atccggccgt ctggacctac aacggcgaga
     7441 aatacgagac ctgcccggtg gacaatatct gggctttgac ggagcccgag tggaaccgca
     7501 gggtcgccat gcccgatccc ttgaacaagc cgtccttcgt cgactggttc aaccagatgg
     7561 agacgcactg ggacgcgaca atcgccgcgg cctacgaggc gcatttcggc cagaagctgg
     7621 acaccagcaa cgagagcgcc acggccgcct gggtccaggc gctggccgag aatgcgcccc
     7681 tggtgacgga ttccgactcg gccgcggccg aggcgatcgg cacgcccggc caggacgagc
     7741 cgttcttcgg catcctggcg acggccaagt atcgggacaa tgccgacggc ggcctgcatc
     7801 tggcgctgtg cgagggcatc gagccgttca tcggcttcgc caacccgtcc tacggcctga
     7861 tcgcgaacgg cacggcgagc cccaatgccg caaagctctt tctccgcttc atgatgacgg
     7921 ccgagggcgt cgagccgatg accgtcgacg gcaaggtttc cggcaacgag gccgtgccgt
     7981 cgcacccgga cgagccctcg ggcgtggcgg ccttcgcctc gcggctgacg ccgcatctgg
     8041 cggcgaccgg cggcgaggac ttcgaccggc gccaggattg gcaggacttc tggcggatcc
     8101 actacgcccg ctgacgtacc ctccagggcg ggccggcatg cccggcccgc ccgtgtgtcc
     8161 gctcatggtg gcgccgaccc attttgatga acccacgaac catagcggag cgagcaagag
     8221 tggcccccat cttccagcga cctgccctga gcctgatatc cgagggccac ggccccgacc
     8281 gtgcttccct cttcatcggc ggcaccgagg ctgcccggga cctggctgcg ctcgacgccg
     8341 ccggcatctc cacggtggtc aactgcgcgg tcaacctgga catcaactac gtccgggatc
     8401 ccgccttcgc ggcggcgccc ggcaagtgcg ccgcgggccc cggggcggtg cgcagctaca
     8461 agatcgggtt gatcgacggc gacggcaatc cccggcagat gctgctggcg ggctactaca
     8521 tcctcgacgg cgccctgacc cagaccatgc ccgacaaggc atcctatccg caccggaccc
     8581 gcggccacgt gctcgtccac tgccgtggcg ggcgcagccg ctcggtcgcc ctggtggcgc
     8641 tctatctgca ttgtcaggag ccggagcgct atccccggct cgacgatgcg atcgcccatg
     8701 tgcggcgccg gcgggagctg cgcgaggagg aatggcacga gacgccgaag ccggtgctgg
     8761 tcgaggccgc acgctacgcc gccgccgcga tcaggctgct cgacgagaca tccctgcatg
     8821 cgcccgccga gcccgaatgc cggcccgcct gagcggcggg ggcgtctacc cgaacgagac
     8881 gccggtcagg aggcggttct cctgggcgat caggttgccg gcctcgcggc tcttggtgag
     8941 gaaggcggtc caggcgggat ctgccgtcat ggcggcgcgg cgctgctcgc ggtcggcggc
     9001 ggattcgtag gcccagacgt ggacataggt gttgatcggg ccggtctcgg tgacgccgta
     9061 gaggacgggc tggcccaggt gcttggtctg ggtcgcgtag ccgtgctcct tgtagagggc
     9121 gagctgggcc gggacggtgc ccgggcggca ggtgtagatg cgcatgtcgt agatcatcgt
     9181 cggcgttccc cgaaatcctt ggcaatggcg gcgaaaggcg ccatggcgcg gcccttgtca
     9241 acccgccatc ggcggtggcg attcccgtcg accggttgtc ggcgagtgcg ctagaagcgc
     9301 tcgacaagca gggaggaaga gatgacgagc ctgaccagac gcggcctgat ggcggcgacc
     9361 gccattgccg gtgtcggcat cgcggcaccg acggtgctcc gggcgcaggg cacggtgatc
     9421 cgctggggcg agatgctgcc ggcgacgcat ccgcaggcgc tgatgatcga ccgcatcgcg
     9481 gcgaccgtgg ccgagcagtc ggatggccgg atcgagatcc agagcttccc gaacggccag
     9541 ctcggctcgg gcaaggacat gatggattcg gtcgtgtccg gggcactggc gatgaccacc
     9601 gacggggcgg cggcgctcgg ctcgctcctg ccgcagctct cggtgatcga ggcgccctat
     9661 ctctggcgcg acgccgcgca catggcgaag gtggccgacg cgccggtctt cgccaagatg
     9721 aacgacgacc tcgtggcgca gcgcgggctg aagatgctgg ccgtcaccta ttacggcaag
     9781 cggcacctga cgaccggcac ccgcgaggtg cacaccgtgg ccgacatgga gggcttcaag
     9841 ctgcgcgtgc cgccggtcga cacgttccgt gcgatggccg aggcctgggg ggcgcaggcg
     9901 acgccgatca acttcaccga gctctatctg gcgctgagcc agggggcggt ggacggccag
     9961 gagaacccgc tgccgaccat cgccagcgcc aagctcaacg aggtgcagaa atacctcgtg
    10021 ctgaccgggc acatcatcac gccgcgcctg gtgatcgcca acaacgactg gctgaacggg
    10081 ctggaggagg cggatcgcga gatcctggag ggggcgatcg ccgaggcggt cgcctggcag
    10141 gatgccgagc tggcgagcca ggaggcgtcg ctccagggca cgctcgagca gcagggcatg
    10201 atcgtcatcg agcccgatgt cgagagcttc aggaagccgg tgctcgagca gctcccgccc
    10261 aagttcgtcg acaagtgggg cgagggcacc tgggacgccc tggcggcgct ctgacgggcc
    10321 ctgccgcccc ggcgtagagc cgtgctccca tcgctcgccc gcgtggcgca gcggcttctg
    10381 gaggtcgccg ccgtcctcct gctgctggcc atgctggcga gcgtcttcct cggcgtcgtc
    10441 ttccgtctcg ccaaccagcc gctgacctgg tcggacgaga tggcgacgta tctgctggtg
    10501 tggaccggct tcgtcggctg gatcatcgcg ggccagcgcg gcagccatat ccgcatcacg
    10561 gcgatcatcg gccggctccg gggcacgccc ctgatgctgg cggaggtcgt catccagctc
    10621 ctggtgctgg tcttcgggct gatcctgctg acgcagagct tcgggctgat ccagcgcaat
    10681 ctcgatgtcg agtgggtgag cctgccgctg tcggtggcgc tggtctacat cccgatcccg
    10741 atcgccggct tcgccgtggt gctgcaggcc ggaatcgaga tccttcggct ggtgcgcgag
    10801 agggcggtct cgtgacggcg atcatggtgg agatcctgat cgtctggttc gtggccctga
    10861 tgctcggcgt gccgctcttc gccacgctgg gcctggcgtc cgccgccttc gtctggctca
    10921 acggcatgcc gatcgacatc gtgccgcaga agctggcgca gtcggtcaac tccttcccgc
    10981 tgctcgccgc ccccctcttc atcctgatgg gggcgatcat gaactcggcc gggatcaccg
    11041 accggatctt cggcttcgcc acggcctgcg tcggctgggt caggggcggg ctctgccatg
    11101 ccaacatcct ggcgagcgtc atcttcgccg gcatgtcggg ctcggccgtg gccgatgccg
    11161 ggggggtggg tgccctcgag atccgggcga tgaagcaggc cggctacgac gccgagacgg
    11221 cggcggcgat caccgcggcc tcggcgacca tcgggccgat catcccgccg tccctgccga
    11281 tggtgatcta cggggtcagc gccgacgtct cgatcggcgg gctcttcctc gccggggtcg
    11341 tgccggggct gctgatggcg ggagcgctct cggccatggt cgtggtgatg gcgcggcggc
    11401 gcaacatggc gcgcgagctc tttccggggc tgcgcgggct ctggctcgcc taccggcggg
    11461 cgcactgggc cttgatgacg ccggtgatcc tcttcggcgg gatgatggcc ggcatcatga
    11521 cgccgaccga ggcggcggcg gtcgccacgg tctatgccct c
//
LOCUS       DAOTFI010000158        11548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6648_length_11548_cov_6.195162, whole genome shotgun sequence.
ACCESSION   DAOTFI010000158 DAOTFI010000000
VERSION     DAOTFI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6648_length_11548_cov_6.195162"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(536..1660)
                     /locus_tag="P5558_15680"
     CDS             complement(536..1660)
                     /locus_tag="P5558_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRY25809.1"
                     /translation="MTPTNAEVARYTDKYFDKTRRIVERFGDCRVTYAVFMRRPVLCT
                     HRLAVDWLREVAAERAADITIEERFPEGGWVGAGEPMLYLHGPFRHLVDLETMFLQKL
                     GPACVAAFNAWTMCTDLPYVGFLAMDARHCAGAEMAEMMAYAASVGSARARRESAAKG
                     FIGNATDATAHFFGQSHGLGTMPHALIGYARSTVEAARMFHATFPDDPLYVLVDYFGR
                     EISDALDVCAAFPDLAPTGRLGVRLDTHGGRFVEQLDTAKSYAVLDRHVPEAIRTYRT
                     HDELRWLTGTGVSAAAIYHMRESLDAAGFPEVKIIASSGFSPSKCKLMASVRAPIDVI
                     GTGSYLPEIWGETYATADVIAYDGEPRVKLGREFLLPSTD"
     gene            complement(1657..3027)
                     /locus_tag="P5558_15685"
     CDS             complement(1657..3027)
                     /locus_tag="P5558_15685"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRY25810.1"
                     /translation="MTQSWTPQSWRQRSRHQMPDYKNAENLAGVERQLAGYPPLVFAG
                     EARKLKEELAQVAKGQGFLLQGGDCAEAFADFNADKIRDTLRVLLQMAVVLTFGGGMP
                     VVKVGRMAGQFAKPRSAPMERIGEVELPSYRGDIVNGLDFVAEDREPDPARQLQAYSQ
                     AAATLNLLRAFTQGGYAALTRVHEWTLGFLDKSPAGERYQDFADAISQALDFMRACGV
                     TDLSTPEITRTTLYTSHEALLLGFEEAMTRIDSTSGRWYDTSAHLVWIGDRTRQLDGA
                     HVEYCRGIANPLGVKAGPTMPVDDLLRLCDVLNPANEPGRLTVIARMGHELVERHLPP
                     LVRALEREGRIVVWTCDPMHGNTVKSSSGYKTRPFERILAEVRSFFAIHRGLGTHAGG
                     IHLEMTGQDVTECTGGAQAITEAALKDRYHTYCDPRLNAQQSLELAFLMAEALKEERQ
                     GLRAAS"
     gene            complement(3059..4459)
                     /gene="gorA"
                     /locus_tag="P5558_15690"
     CDS             complement(3059..4459)
                     /gene="gorA"
                     /locus_tag="P5558_15690"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HRY25811.1"
                     /translation="MARFDLYVIGGGSGGVACGRRAAAYGAKVGLAESSRVGGTCVIR
                     GCVPKKLMHYGAHFAEMFRLAKGYGWRLGEAPVLDFQALIEARNTEIQRLNDIYLGML
                     DRAGVTVHADRANVLGRTADGAFRIAVGGAEHEATNLLVAVGARPAKPVLPGVEHTAT
                     SDEILENLYPMPKRLAVVGAGYIGVELASIFNGLGVQTSLILRADQPLRGFDEDLRRH
                     MTAELERHGLDLRRNTVVTGIAPAGAGYRLDTTTGAIDADLVVYATGRDALPNTSQLG
                     LDALGVAMDPLGAIEVDTAYKSSVPGLYAVGDCSDHAGQGLHSGQHDLTPVAIAEGRV
                     IAETLYNDNPHEVAYASIPTAIFGMPQAGSVGMSEAEARSRGHAVQVFKTDFRPMLYT
                     LPGAANRTFMKLVVDADSDRVLGCSMVGDDAAEIVQGLAIALTAGATKAQFDATVGVH
                     PSAAEEFVTMYQPATG"
     gene            4645..6240
                     /locus_tag="P5558_15695"
     CDS             4645..6240
                     /locus_tag="P5558_15695"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY25812.1"
                     /translation="MDKRLKYWGWGHEGDGLDAGQTRELLATFVDGFAIRPTDDGAFP
                     TLDALDLPAPRLTPPASVAGLCTTDRHERALHAFGQSQPDSIRCYMGDFAHAPDVVAY
                     PENALDIARLMEWCGVAGAALIPYGGGSSVVGGVTADVDDGFNGVVALDMGRMGRVLE
                     IDEVSRAARIQAGARGPALEAGLRPSGLTLRHFPQSFEHSTLGGWIATRSGGHFATLL
                     THIDDLVESVAMTTPAGEIASRRLPGSGAGPSPDRFMIGSEGALGVITEAWVRLQGRP
                     VFKATAGYRFADFLDAARAVRAVAQAGLYPANLRIVDGVELQVNGAGDGSFTLMVVSF
                     ESADHQVDAWMARAEECCLDHGGRVDVPWRDNPNAHLEGAVGAWREAFIRMPYNRERL
                     TPRGIISDTFETSITWDRFEGFHETIREATAQAIRDATGRPGAVSCRFSHAYPDGPAP
                     YFAFHAAGRKGALLGQWQAIKDAVSEALIREGGTITHHHAVGRDHQKWYERQRPALFG
                     EVLAAAKKRLDPLGILNPGVIVR"
     gene            complement(6681..7631)
                     /locus_tag="P5558_15700"
     CDS             complement(6681..7631)
                     /locus_tag="P5558_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY25813.1"
                     /translation="MAEIHAVSLQQVAPFLAQRGIDVLEFFGRELVAPSIFQARDTWF
                     PRAECLRLTNAVARETGDPCAGAMIGKSARLQDFGEFGRRVTDADTVEGACRAMIGNL
                     GLVHRGSGLTAGREGDRIFLRFALEGASTQDTTQFVQASLAVIRNILLLSGEADMVTV
                     RLAVPYAPQWAALEAPLGERLEFGCAAEGVLFPRSLLEKPLASANAPSRDHDALRVTL
                     RAAQIIAAMFPETPIRIDGVASRLGLTRRTLQRRLKLCGVVFSDLLDVARRDQALRSL
                     ADRSQDLLGLALDLGYGDPAHFTRAFKRWTGTTPSAFRGR"
     gene            7803..9365
                     /locus_tag="P5558_15705"
     CDS             7803..9365
                     /locus_tag="P5558_15705"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HRY25814.1"
                     /translation="MADRGRGQQPKGGEMLAPSRICRATASVVACVVIGGWFIVARAE
                     TYKYETAIPPGIAAPDSIETPLGTLNFHNGFPDEATARTLFDNLDLQRAVQAFLLGIP
                     AVSQVANRAAFAGLGPANSFVPIFEQLLDSRTLVLTGNDNTVYSWTWLDLSDGPIVLE
                     VPPKVLGTANDMWQRWVVDVGITGADKGEGGKYLFLPPGYSGDVPADGFVQVVHSPTF
                     GLWIPWRSFLVDGDPAPGVDLVKRHTKIYPLGAAPATPSFVDMTGTAISTVYPADFTF
                     WELLNEVVQQEPVASLDPVTLGFFAAIGIEKGKPFAPDDRMKKILGQAAAIGDATSRA
                     IVFHNRDANAFYYRGSNWQRGFTGGYNFQSQPGVADFDAATFYYTIAILVTPAMEEKF
                     IGKGSQYAWVARDAADAPLDGGKTYRLHLPKDVPVKDFWSLILYSNQTRSMIQTDQPH
                     PAVSSQDKDLLVNADGTIDVWFGPQAPEGKKPNWVQTVPGSGWNLVLRLYGALEPWFD
                     QSWRPGEIEPVP"
     gene            9467..10933
                     /locus_tag="P5558_15710"
     CDS             9467..10933
                     /locus_tag="P5558_15710"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HRY25815.1"
                     /translation="MALLPGGAGAQPAYAPDVPAKITTPDTVETRIGTLHFKNGVPDA
                     TTAQMAFDQLDFGRGVQAFLTGMSATSVHALCRGFEEAGIPLNRGIGISEDLLDARSL
                     FLTANTTTVYVVQCVDLTEGPMVVRIPPRVLGPVDDANFRWVTDVGLTGPDKGAGGDY
                     LFVPPGYTAPLPAEGYHIARPGTNRLLIFYRAFVEKGDVAAAVAGVKAKAAVFALSRA
                     DGPPATTVVDVSGVKFNTISANDVSFFEELNAVVQNEPADWVDPDTVGLYASIGIRKG
                     QSFAPDDRMKGILTDAVAVANAIARTNLFASRDPRTRIYADRQWFTPFVGGSYQFLDG
                     AERLLDARMMFFYYATGITPAMTDSRPGTGSAYAISVRDAAGAYLEGGKTYQVTLPGP
                     IPAKDFWSFTVYDNQTRSLLPTDQRLAGIDSTVAGVEMNPDDGVTIWFGPEAPAGHEK
                     NWVQTMPDQGYNVALRLYGPLEPWFDQTWRPGDPVPAP"
     gene            10975..11493
                     /locus_tag="P5558_15715"
                     /pseudo
     CDS             10975..11493
                     /locus_tag="P5558_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558295.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1785 a         3868 c         4051 g         1844 t
ORIGIN      
        1 tctgtcttga caacatcaac ctatcggatg gagctgggcg atgccacgct tcacctctca
       61 aataccgagc aattccgccc cttcagggcg ttttgcaact gcctctcgtg tccctgagtt
      121 ccccctcgtg tccctgagtt tcccgaggcc ttgcgcagga tctcgttggc cgggcgcagc
      181 tcgcgaacct cacgctccaa cgccttcagc cgagcccgct cgtcagtggt cgcgcagtcc
      241 cgcagacctg aaagctgcct ttcgccagat caatcgccag tagaccgatc tgattggtcg
      301 tcttcgtgcc ctccacatgg ttcgtccgga accgtcatgg cataagagat gccgctcggt
      361 gggggcatcc actgcatcag ttcagaaccg acttttcaga ggggttaaac tcagttcgtg
      421 tcccgacttt tccagttcgt gtcccgactt ttccaccgac ttttccaaag gtcaggcgcg
      481 tccggcgccg ctgcgaccgg agtgtcccga ggagagccaa tgcccgggca ggctcctagt
      541 cggtggacgg cagcaggaac tcgcggccga gcttgacgcg gggctcgccg tcataggcga
      601 tcacgtcggc ggtggcatag gtctcgcccc agatctcggg caggtaggag ccggtgccga
      661 tgacgtcgat gggtgcccgc acgctcgcca tcagcttgca cttggacggg ctgaagcccg
      721 agctggcgat gatcttgacc tcggggaagc cggccgcgtc gagcgattcg cgcatgtggt
      781 agatggcggc ggcggaaacc ccggtgccgg tcagccagcg gagctcgtcg tgggtgcgat
      841 aggtgcggat cgcctcgggc acgtgccggt cgagcacggc ataggatttc gccgtgtcca
      901 gctgctcgac gaagcggccg ccatgggtgt cgaggcggac gccgaggcgg ccggtcgggg
      961 cgaggtcggg gaaggccgcg cagacgtcga gcgcatcgct gatctcgcgg ccgaaatagt
     1021 cgaccagcac atagagcggg tcgtcgggga aggtggcatg gaacatgcgc gccgcctcga
     1081 ccgtgctcct ggcgtagccg atgagcgcgt gcggcatggt gccgaggccg tggctctggc
     1141 cgaaaaaatg ggcggtggcg tcggtggcgt tgccgatgaa gcctttcgcg gcactctcgc
     1201 gccgggcccg ggccgagccg acgctcgccg cataggccat catctcggcc atctcggcac
     1261 cggcgcaatg ccgcgcgtcc atggcgagaa agcccacgta gggcaggtcg gtgcacatgg
     1321 tccaggcgtt gaaggcggcg acgcaggcgg ggccgagctt ctgcaggaac atggtctcga
     1381 ggtcgacgag atggcggaac ggcccgtgca gatagagcat gggctcgccg gcgccgaccc
     1441 agccgccttc cggaaagcgc tcctcgatgg tgatgtcggc cgcgcgctcg gccgccacct
     1501 cccgcagcca gtcgaccgcc agccggtgcg tgcagagcac cggccggcgc atgaagaccg
     1561 cgtaggtcac ccggcagtcg ccgaaccgct cgacgatgcg ccgcgtcttg tcgaaatact
     1621 tgtcggtgta gcgggcgacc tcggcgttgg tcggcgtcat gacgcggcgc gcaggccctg
     1681 ccgctcctcc ttcagggcct cggccatgag gaaggcgagc tcgaggctct gctgcgcgtt
     1741 cagccgggga tcgcaatagg tgtggtagcg gtccttcagc gccgcctcgg tgatcgcctg
     1801 cgccccgccg gtgcattcgg tgacgtcctg ccccgtcatc tcgagatgga tgccgccggc
     1861 atgcgtgccg aggccgcggt ggatggcgaa gaagctcctg acctcggcca ggatccgctc
     1921 gaacgggcgg gtcttgtagc ccgacgatga cttgaccgtg ttgccgtgca tgggatcgca
     1981 ggtccagacg acgatgcggc cctcgcgctc gagggcgcgg accaggggcg gcaggtgccg
     2041 ctcgaccagc tcgtgcccca tgcgggcgat gacggtgagc cggcccggct cgttcgccgg
     2101 gttgaggacg tcgcagagcc gaagcaggtc gtcgaccggc atggtcgggc cggccttgac
     2161 ccccagcggg ttggcgatgc cgcggcagta ctcgacatgg gcaccgtcga gctgacgggt
     2221 gcggtcgccg atccagacca ggtgcgccga ggtgtcgtac cagcggcccg aggtggaatc
     2281 gatccgggtc atcgcctcct cgaagccgag cagcagcgcc tcgtggctgg tgtagagggt
     2341 ggtccgggtg atctccggcg tggagaggtc ggtgaccccg caggcgcgca tgaagtccag
     2401 cgcctggctg atcgcgtcgg cgaaatcctg gtagcgctcg cccgccggcg acttgtcgag
     2461 aaagcccagc gtccattcgt ggacgcgggt gagggccgcg tagccgccct gggtgaaggc
     2521 gcgcagcagg ttgagcgtcg cggcggcctg gctgtaggcc tggagctgcc gcgccgggtc
     2581 gggctcgcgg tcctcggcga cgaagtcgag gccgttgacg atgtcgcccc tatagctcgg
     2641 cagctcgacc tcgccgatgc gctccatggg tgcggagcgc ggcttggcga actggccggc
     2701 catgcggccg accttgacca ccggcatgcc gccgccgaag gtcaggacca cggccatctg
     2761 cagcaggacg cggagcgtgt cgcggatctt gtcggcgttg aagtcggcga aggcctcggc
     2821 gcagtcaccg ccctggagca ggaagccctg gcctttcgcg acctgggcca gctcctcctt
     2881 gagcttgcgc gcctcgccgg cgaacaccaa aggcgggtag ccggcgagct gacgctcgac
     2941 gccggccaga ttctcggcgt tcttgtagtc cggcatctgg tggcggctcc tctgccgcca
     3001 gctctgtggt gtccagctct gggtcatggc ccttcgtccc tattgccccg tcaggcgatc
     3061 agccggtcgc cggctgatac atcgtgacga attcctccgc ggccgatggg tgcaccccga
     3121 ccgttgcgtc gaattgcgcc ttggtggccc cggccgtcaa ggcaatggcg agaccctgca
     3181 cgatctcggc cgcatcgtcg ccgaccatcg agcagccgag cacacggtcg ctgtcggcgt
     3241 ccaccacgag cttcatgaag gttcggttgg cggcacccgg cagcgtgtag agcatcggcc
     3301 ggaagtcggt cttgaagacc tggaccgcgt ggccgcggct tcgggcctcg gcttcgctca
     3361 tgccgaccga gccggcctgc ggcatgccga agatggcggt cgggatcgag gcgtaggcga
     3421 cctcgtgcgg attgtcgttg tagagggtct cggcgatcac ccggccctcg gcgatggcga
     3481 cgggcgtgag gtcgtgctgg ccgctgtgca ggccctggcc ggcgtggtcg ctgcagtcgc
     3541 cgacggcgta gaggccggga acggagctct tgtaggcggt gtccacctcg atggcaccta
     3601 gggggtccat ggcgacgccg agcgcgtcga ggccgagctg gctggtgttg ggcagggcgt
     3661 cgcggccggt ggcgtagacg acgaggtcgg cgtcgatggc gccggtggtg gtgtcgagcc
     3721 ggtagccggc gccggcgggt gcgatgccgg tgaccacggt gttgcggcgg agatcgaggc
     3781 cgtggcgctc gagctcggcg gtcatgtgcc ggcggagatc ctcgtcgaag ccgcgcaagg
     3841 gctggtcggc gcgcaggatc aggctggtct ggacgccgag cccgttgaag atgctggcga
     3901 gctcgacgcc gatatacccc gcccccacca cggctagccg cttcggcatg gggtagaggt
     3961 tctcgaggat ctcgtcggag gtggccgtgt gctcgacgcc ggggaggacg ggcttcgccg
     4021 gcctcgcgcc cacggcgacg agcaggttgg tggcctcgtg ctcggcaccg cccacggcga
     4081 tgcgaaaggc gccgtcggcg gtgcgcccga ggacattggc acggtcggca tgaacggtga
     4141 cgccggcgcg gtcgagcatg ccgagataga tgtcgttgag ccgctggatc tcggtgttgc
     4201 gcgcctcgat gagggcctgg aagtcgagca ccggcgcctc gccgagccgc cagccatagc
     4261 ccttggccag gcggaacatc tcggcgaaat gggcgccgta atgcatcagc ttcttcggca
     4321 cgcagccgcg aatgacgcag gtgccgccga cccggctgga ttcggcgagc ccgaccttcg
     4381 ccccgtacgc ggcagcacgg cggccacagg cgacgccgcc ggagccgccg ccgatgacgt
     4441 agaggtcgaa acgagccatg ccgaatcccg aagcgctctc gtggaatgcg ggctcctagc
     4501 gcgtccgccg gcggctgtca cgttttcggc ggcgccagac gggtctcgag cgggctcgat
     4561 cggcttgctc gccgcggccg gccggtccac gttcgcacgg gaacgccggg gcgggcaggc
     4621 ttgatacggg atcgggaagg cgccatggac aagcggctga aatactgggg ctgggggcat
     4681 gagggggacg ggctcgacgc gggccagacc agggagctgc tggccacctt cgtcgacggc
     4741 ttcgccatcc ggccgaccga cgacggcgcc tttccgacgc tcgacgctct cgacctgccg
     4801 gcgccccggc tgacgccgcc ggcgagcgtc gcgggcctgt gcacgaccga tcggcacgag
     4861 cgggcgctgc acgccttcgg ccagtcgcag ccggattcga tccgctgcta catgggcgac
     4921 ttcgcccatg cgccggacgt ggtggcctat ccggagaacg ccctggacat cgcgcggctg
     4981 atggaatggt gcggcgtcgc gggtgcggcg ctgatcccct atggcggcgg ctcctccgtc
     5041 gtcggcggcg tgacggccga tgtcgatgac gggttcaacg gcgtcgtcgc cctcgacatg
     5101 ggccgcatgg gccgggtgct ggagatcgac gaggtcagcc gggcggcgcg gatccaggcg
     5161 ggtgcccggg ggccggcgct ggaagccggg ctcaggccga gcgggctgac gcttcggcac
     5221 tttccccaga gcttcgagca ctcgacgctg ggcggctgga tcgccacccg ctcgggcggg
     5281 cacttcgcga ccctcctcac gcatatcgac gacctcgtcg agagcgtcgc catgaccacg
     5341 ccggcgggcg agatcgcctc gcggcggctg ccgggctccg gtgccggccc cagcccggac
     5401 cggttcatga tcggctcgga gggcgcgctc ggggtcatca ccgaggcctg ggtgcggctg
     5461 caggggcggc cggtcttcaa ggcgacggcg gggtatcgct tcgccgattt cctggatgcg
     5521 gcgcgggcgg tgcgcgccgt ggcgcaggcc gggctctatc cggccaacct gcgcatcgtc
     5581 gacggggtcg agctgcaggt gaacggcgcc ggcgacgggt cgttcacgct catggtcgtc
     5641 tcgttcgaga gcgcggacca tcaggtcgac gcctggatgg cgcgggcgga agaatgctgc
     5701 ctcgaccatg gcggccgggt cgacgtgccc tggcgggaca acccgaacgc ccatctcgag
     5761 ggggccgtcg gcgcctggcg cgaggccttc atccgcatgc cgtacaaccg cgagcggctg
     5821 acgccgcgcg gcatcatctc cgacacgttc gagacgtcga tcacctggga ccggttcgag
     5881 gggttccacg aaaccatccg ggaggcgacg gcacaggcga tccgggacgc gacgggccgg
     5941 ccgggggcgg tctcctgccg cttcagccac gcctatccgg acgggccggc accctacttc
     6001 gccttccatg ccgcgggccg caagggggcg ctcctcgggc agtggcaggc gatcaaggac
     6061 gcggtgtcgg aggcgctgat ccgcgagggc gggacgatca cccaccacca cgcggtcggc
     6121 cgcgatcacc agaaatggta cgagcggcag cgaccggcgc tgttcggcga ggtgctggcg
     6181 gcggcgaaga agcggctgga cccgctcggc atcctcaacc cgggcgtcat cgtgcggtag
     6241 cggtgtgtca tggccctgct gttggcgggc gggacggcgg cgtggcgccg ggtggtgggg
     6301 cggcagggtg tcgaggcggg tcggtcgggc ggggcggcag cgggatggcg ggggatggtg
     6361 gtgccttggt ctcaccggcg cggcgggcgg tggcggccgg gacggtgctt cggcgagggg
     6421 gctggagggc catggccgtc gcttgagccg ggcgcctgcc gtcggagacc gggttgccac
     6481 gggcccggat gcccaaccgt gcggctggca ccgacgccga gctcgcggct catcgccgcc
     6541 acgttctcgc cggcctccat gcggccgacc gcacccagct tgaaatcacg gtcgtagcgt
     6601 cggatcgtcc gggtcctcgt cgcgttctcc cggcgcagaa ccgacacgtc gttctcgtct
     6661 cacccgaggg gcgccgtcca ctaccgtccc cggaaggcgg agggcgttgt gcccgtccac
     6721 ctcttgaacg cccgagtaaa atgggccgga tcgccgtagc cgaggtccag tgccaacccc
     6781 aggagatcct gggatctgtc ggcgaggctg cgaagtgcct ggtcgcgccg cgcgacatcg
     6841 aggagatccg agaacaccac cccgcacagc ttgagcctgc gctgcaacgt acgccgcgtc
     6901 agccccagtc tggacgcaac gccgtcgatg cggatcggag tctcggggaa cattgccgca
     6961 atgatctgtg ccgcccgcag cgtgacccgc agcgcatcgt ggtcccggct cggagcgttc
     7021 gcgctggcga gcggcttctc caacagcgat cgggggaaaa gcacgccctc ggcggcgcag
     7081 ccgaactcca gcctttcgcc aagaggagcc tcgagagcgg cccactgcgg ggcgtaggga
     7141 acagcaagac ggaccgtcac catgtccgct tcgccggaga gcaggagaat gtttcggatc
     7201 accgcgaggg aggcctggac gaactgcgtg gtgtcctggg tcgaggcccc ctcgagcgcg
     7261 aagcgcagaa agatgcggtc gccttcccga ccggcagtca gccctgagcc acggtgcacg
     7321 aggccgaggt tgccgatcat cgcccggcac gccccttcga ccgtatccgc gtcggtcacc
     7381 cgcctgccga actcgccgaa atcctgcagg cgcgccgact tgccgatcat cgcaccggcg
     7441 cagggatcgc ctgtctcgcg cgccacggca ttcgtcagtc gcaggcactc ggcgcgcggg
     7501 aaccacgtat cgcgggcctg aaaaatactc ggcgccacca gctcgcggcc gaagaactcg
     7561 agcacgtcga tgccgcgttg cgccaggaag ggcgcaacct gctgcaggct caccgcgtgg
     7621 atctcggcca tcctcaactc tccgggcgac tgcccctcag ccaccgccga ggttgtcagc
     7681 ggctggattc cagaaattgt caccaaatgg caagcgcaac ccctgcctcg gcaagtaagc
     7741 tcgccagcag cccgcctgtt ggccgacccc gcccatcggg ccgctcgccc cgaccggcgc
     7801 tgttggcaga tcgcggcagg ggccagcaac ccaagggagg cgaaatgctc gcaccatcca
     7861 ggatatgccg cgctaccgca tcggtggtcg cctgcgttgt gatcggcggc tggttcatcg
     7921 tggcacgggc ggagacatac aagtacgaga ccgcgatccc gccggggatc gcggcgccgg
     7981 acagcatcga aacgcccctc ggcacactga atttccacaa cggcttcccg gacgaggcga
     8041 cggctcggac cttgttcgac aatctcgatc tgcagcgtgc cgtgcaggcc tttctgctcg
     8101 ggattccggc cgtcagccaa gtcgcgaacc gcgccgcctt tgccggcctc ggcccggcca
     8161 acagcttcgt gccgatcttc gagcagctgc tcgactcccg cacgctggtg ctcaccggca
     8221 acgacaacac cgtctacagc tggacctggc tcgacctgag cgacgggccg atcgtgctcg
     8281 aagtgccgcc caaggtgctc ggcaccgcca acgacatgtg gcaacgctgg gtcgtcgacg
     8341 tgggcatcac cggcgccgac aagggggagg gtggcaagta cctgttcctg ccgcccggct
     8401 actcgggcga cgtgccggcc gatggcttcg ttcaggtcgt gcactcgccg accttcggcc
     8461 tctggatccc ctggcgcagc ttcctggtgg acggcgatcc ggcgccgggc gtggatctgg
     8521 tcaagcgcca cacgaagatc tacccccttg gcgcggctcc ggcgacgccg tctttcgtgg
     8581 acatgaccgg aacggcgatc agcaccgtct atccggcgga tttcaccttc tgggaattgc
     8641 tgaacgaggt cgtgcagcag gagccggtcg catcgctcga cccggtgacg ctcggcttct
     8701 tcgccgcgat cgggatcgag aagggcaagc ccttcgcgcc cgacgaccgg atgaagaaga
     8761 tcctcggcca agccgccgcg atcggcgacg cgacgtcgcg cgcgatcgtg ttccacaatc
     8821 gggatgcaaa tgccttctac tatcgaggca gcaactggca gcgcggcttc actggaggct
     8881 acaacttcca gtcgcaaccg ggagttgcgg atttcgacgc cgcgaccttc tactacacga
     8941 tcgccatcct ggtgacgccg gcgatggagg agaagttcat cggcaagggc tcgcaatacg
     9001 cctgggtggc tcgcgatgcc gccgacgcgc cgctcgacgg cggcaagacc taccggctgc
     9061 atctgcccaa ggacgtgccg gtcaaggatt tctggtcgct catcctctac tcgaatcaga
     9121 cccgctcgat gatccagacc gaccagccgc atccggccgt ttcgagccag gacaaggatc
     9181 tgctcgtgaa tgccgacggc acgatcgacg tctggttcgg accgcaagca ccggagggca
     9241 agaagccgaa ctgggtccag accgtgccgg ggtcgggctg gaacctggtg ctgcggctct
     9301 atggcgcgct cgagccctgg ttcgaccaga gctggaggcc cggggagatc gagccggtgc
     9361 cctgaggcag gggcgccggc tgcccggtga agcagcgtac agcgagaggg gagccggaat
     9421 gaccgtgcct cgacagttcc tgatggccag cgcccttgcg gcggcagtgg cgctcttgcc
     9481 cggcggggcc ggcgcgcagc cggcatacgc gcccgacgtg ccggcgaaga tcacgacgcc
     9541 cgacacggtc gagacccgga tcggcaccct gcatttcaag aacggcgtgc ccgacgccac
     9601 cacggcgcag atggcattcg accagctcga tttcgggcgt ggcgtgcagg ccttcctcac
     9661 gggcatgtcg gccacctcgg tgcacgccct gtgccggggc ttcgaggagg ccggcattcc
     9721 cctcaaccgc ggcatcggca tcagcgagga tctgctcgat gcccgctcgc tgttcctgac
     9781 cgccaacacc accactgtct atgtcgtgca atgcgttgac ctgacggagg ggccgatggt
     9841 cgtgcgcatc ccgccgcggg tgctgggccc cgtcgatgac gccaatttcc gctgggtcac
     9901 cgacgtcggg ctgaccggcc ccgacaaggg ggcgggcggc gactatctgt tcgttccgcc
     9961 cggctacacg gcgcctctgc cggcagaagg ctatcacatc gcacggccgg gcacgaaccg
    10021 gctgctgatc ttctatcgcg ccttcgtcga gaagggcgat gtcgcggcgg cggtggcggg
    10081 tgtgaaggcc aaggcagcgg tctttgccct ctcccgggca gacggcccgc cggcgaccac
    10141 ggtcgtcgat gtctcgggtg tgaaattcaa cacgatcagc gccaacgacg tcagcttctt
    10201 cgaggaattg aacgcggtgg tgcagaacga accggcggat tgggtcgatc ccgataccgt
    10261 gggcctctat gcgtcgatcg gcatccgcaa ggggcagagc ttcgcgcccg atgaccggat
    10321 gaagggcatt ctcaccgatg ctgtcgccgt cgcgaatgcc atcgcgcgca cgaacctctt
    10381 tgcctcgcgc gatccgcgca cgcgcatcta cgccgaccgg cagtggttca cgccgttcgt
    10441 cggcggcagc tatcagttcc tcgacggcgc cgagcgcctg ctcgatgcca ggatgatgtt
    10501 cttctactac gcgaccggga tcacgccggc gatgaccgac tcccggccgg gcaccgggtc
    10561 ggcctatgcg atcagcgtgc gtgatgcggc cggagcctat ctggagggcg gcaagaccta
    10621 tcaggtgacc ctgccgggac ccattcccgc aaaggatttc tggtccttca cggtctatga
    10681 caaccagacc cgctcgttgc tgccgacgga tcagaggctc gccggcatcg acagcaccgt
    10741 cgctggcgtc gagatgaacc cggacgacgg cgtcacgatc tggttcggcc ccgaggcgcc
    10801 ggcgggtcac gagaaaaact gggtccagac catgcccgat cagggctata atgtggctct
    10861 tcgcctctat gggccgctcg agccgtggtt cgaccagact tggcgccccg gcgacccggt
    10921 gccggcaccc taggacagga gacaccgcca ggaagcccgc aagacgagca gcagaagctg
    10981 ccgctcaccg ctcggttgtg ggcggcgttc ttgatggcga tgcactcgaa cggcctctcg
    11041 gtgctgcagc tccgattgca acccgggctt ggctcctgca agagcgcccg gctgctcgcc
    11101 gccaagctcc ggagagccat ggtcaacccg gatcgcgcgc cgatcgcggg tctcgtcgag
    11161 gtcggcgagg acaacgcgac cgcaaggacc gatggctggt cggctgtccc ggcgcgccgg
    11221 gcatcgtcca cgatcccctt accgtcgaac ccatggccgc ccacatcgtt ctgccgtggg
    11281 cgcatcgcgt gctctcgaac ttcgagaccc ggggcctcgt cgtccatcac ggcctccggc
    11341 gcaagcatct ccaacgctct ctcgacgagc tcgtcttccg cttcaactac cgccgcaccc
    11401 gccacgccgc cttttgagcg ctcctccaaa tcaccgccat ccgcaacccg atcacctgac
    11461 aatatgctga tcaaaccgga agcatcggga taagctgaac cgccccgggt ttgccggagg
    11521 ctccaactct tgagaggatg gagcgatg
//
LOCUS       DAOTFI010000159        11522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6667_length_11522_cov_7.844946, whole genome shotgun sequence.
ACCESSION   DAOTFI010000159 DAOTFI010000000
VERSION     DAOTFI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6667_length_11522_cov_7.844946"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /gene="glmM"
                     /locus_tag="P5558_15720"
     CDS             complement(<1..1094)
                     /gene="glmM"
                     /locus_tag="P5558_15720"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRY25816.1"
                     /translation="MQRRMFGTDGIRGTSNTHPMTAEVALALGRAAGGLFRRAGQHGR
                     RHENRVVIGKDTRLSGYMIENALTAGFLSAGMRVMLTGPVPTPAVAFLTRSLRADLGV
                     MVSASHNPYEDNGIKLFGPDGFKLSDALEAEIEARMEAMAADGEAPPDEIGRAMRYED
                     ARGRYVENLKSSFPRGAELGGRKIVVDCANGSAYHIAPELFFELGAEVVGLGVQPNGR
                     NINAGCGSTYPETIKAAVLEHKADLGIALDGDADRLILVDEQGQLVDGDQLLALIAQS
                     WMADGRLRGDGVVATLMSNFGLEGFLRGLGLDLHRTRVGDRYVVERMREGGFNVGGEQ
                     SGHIVLSDFATTGDGLLAALQVLAVLCRSQR"
     gene            1272..1493
                     /locus_tag="P5558_15725"
     CDS             1272..1493
                     /locus_tag="P5558_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25817.1"
                     /translation="MAAARDTPFMPVEIIRPSGQVERPERRQGAAGFRPYVAGDFLPP
                     GSPPNPVERRERRRSRGADQRGRRIDIVS"
     gene            complement(1584..2192)
                     /locus_tag="P5558_15730"
     CDS             complement(1584..2192)
                     /locus_tag="P5558_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRY25818.1"
                     /translation="MIRKTMRIGEVRTSIKLESEFWEYLKAVAQQRHLRLSGLVNEVA
                     SATPERTNLASTLRTYALVHARLRTETMQRDVDKLALAGNTQDLVRVLEACPLPSLII
                     DGDRAIRQMNRAFAFWLNLDPKATVGQRLDNIMILRGPGMRDMWTSLLDGRMARAGFN
                     ATYVSPGKVRTAQAIAVALSAPSVDPARRGCIVMFETLAGRT"
     gene            2346..4781
                     /locus_tag="P5558_15735"
     CDS             2346..4781
                     /locus_tag="P5558_15735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HRY25819.1"
                     /translation="MTERTGSDWPVDGLEPIVVAQAESAQEASGQPRVLVQEPAAVEE
                     VEAVAAPASTPVDPETLGIAPVDAGERRVIAVTPGTTVVLDDPAFDPSRAVYAVDGLD
                     LVVTQPNGGVLVLVGFFAPSEAPPELSVLGAPATSAPVLLQQAEAAPVLDDQSGVEPA
                     AGPVTGPQGGGGAGFTAYDAGSIGEGLDPLGPLGPTSLLYSAPDPEVGNTFVGDEDGN
                     GGINPPPPTGPVVTMQSNISATVGEVSGPGFNPKTTPVLPLSQSALTPDGVDREGLSY
                     QKWLASKGPIDPLALDSINSVDEGNVTLDIEREVFIRFVNENSYSVDSVFVHDIAPDG
                     SIVNVRLAFDGTNVPQDAFAELKTVQPGTEISLGRFAAGTPIGILFLNDGFRENDFAK
                     LQGGRYEVRDPLTGEIAKITDVHPTDKPQLNPLLVHIADDGTETVLKGQDGNRIFSAD
                     ATPASPNQNVINPDGLGHFVSGWDAGNGLLLAGVDDGIGTFRDLSFDDLVIGVRFGSP
                     LNNTLVIEAFENGVDIQITDADSTEMASATIALNGFAGDTLLLDPSVAAGTGITVTQV
                     SDTLIELQGLASIDAYQQVINAASIGIDLQNPEFGERQIEVGVTDPDGLTGTGTTTID
                     VQNNLLTGPGDGSGNDTIIGTAPGDGQSGDDVISGRGGDDTIFGMGGNDFVDGGDGLD
                     TIDVSQPGMNTVIGGPQPDRIVLGPGADVVRITGLSDGADTISFFNASEGDKLDLVEL
                     FRDSDITAGNIADYVQTGAISGGISVQVDLDGRGGDARFVDIAYLQNTTGVVAGGDPS
                     SFVIIPGAEPPVS"
     gene            4990..5934
                     /gene="mdh"
                     /locus_tag="P5558_15740"
     CDS             4990..5934
                     /gene="mdh"
                     /locus_tag="P5558_15740"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRY25820.1"
                     /translation="MARRKIALVGAGQIGGTLALLAGLKDLGDVVLFDVAEGMPAGKA
                     LDLVQSSPVEGFDARLTGTNDYAGIAGADVVIVTAGIPRKPGMSRDDLIATNAGVIKT
                     VGEAIKQHCPDAFVIVITNPLDAMVWVMQKVSGLPAARVVGMAGVLDSARFRWFLAEE
                     FDVSVEDVTAFVLGGHGDSMVPLVRYSTVAGIPLPDLVKMGWTTQDKLDAIVQRTRDG
                     GAEIVGLLKTGSAFYAPAASAIAMAESFLKDKKRVLPCAAQLSGQYGVDGLYVGVPVV
                     IGAGGVERIVEIELDPAERAAFDKSVAAVKGLCDAVQL"
     gene            5946..7115
                     /gene="sucC"
                     /locus_tag="P5558_15745"
     CDS             5946..7115
                     /gene="sucC"
                     /locus_tag="P5558_15745"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRY25821.1"
                     /translation="MNIHEYQAKALLADYGVATLAGRVAYTPEEAEQAARALGGPVWV
                     VKAQIHAGGRGKGGGVKLARSVEEVRQLADQILGMTLVTHQTGPQGRVVKRVYIEDGC
                     DIARELYLGAVIDRASSRITFMASTEGGVEIEEVAATHPEKIIKVAIDPVTGFMPFHG
                     RQLAFGLGLEGAAVRKAVAFMQGLYNCIVERDASLVEINPLVVTGAGDIVALDAKMNF
                     DSNALYRQPKVVDLRDLDEEDPHEIEASRHELNYIKLDGNIACMVNGAGLAMATMDII
                     KLFGGEPANFLDVGGGATKERVTAAFKLLLSDPNVEGVLVNIFGGIMRCDVIAEGVVA
                     AAREVNLNVPLVVRLEGTNVELGKQILADSGLSITPADDLADAAEKIVAAVRGVS"
     gene            7115..7993
                     /gene="sucD"
                     /locus_tag="P5558_15750"
     CDS             7115..7993
                     /gene="sucD"
                     /locus_tag="P5558_15750"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRY25822.1"
                     /translation="MAVLIDASTKVICQGFTGAQGTFHSEQAIAYGTRMVGGVTPGKG
                     GEKHLGLPVFDTVKAAAEGTGADASVIYVPAPFAADAILEAIEAGIGLVVCITEGIPV
                     LDMVKVRRALTGSTTRLVGPNCPGVITPGGCKIGIMPGHIHKPGSIGIVSRSGTLTYE
                     AVAQTTAAGLGQSTCVGIGGDPVKGMEFIDVLELFLADDATQGIIMIGEIGGTAEEDA
                     AEFLRSQPVKKPVAGFIAGRTAPPGRRMGHAGAIISGGKGGAEEKLEAMRAAGIVIAE
                     NPAGIGKAMVEALGRA"
     gene            8061..10913
                     /locus_tag="P5558_15755"
     CDS             8061..10913
                     /locus_tag="P5558_15755"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRY25823.1"
                     /translation="MATRKELEQTSFLYGGNSSFIEELYARYLNDPASVEPSWRGYFD
                     GLDGEDRALFGRARAALQPRPRSLAEPANLNRPISAGLESDATKTLIREHLRLIMLIR
                     AYRVRGHLIAKLDPLKISHKAHHPELDYHSYGFTDADLDREFYLDFVLGLETATLRQV
                     MDVLQKTYSSTVGIEFLHIQDPDQKSWLQQRMESAQGLFDTTPEEKREILEHLTETEG
                     FEQFLHVKFPGTKRFGLDGGESAIAALETMIRTSAELGVDEIALGMPHRGRLNVLANV
                     MGKPYAAIFSEFQGAASYDDVLGSGDVKYHLGTSTDRVLPDGREIHLSLTANPSHLEA
                     VNPVVMGKVRAKQRQKNDTERSRVLGILMHGDAAFAGQGVVHECLEMSELRGYRVGGT
                     LHLIVNNQIGFTTSPAYARSSPYPSDVARGIQAPIFHVNGDDPVAVTHVSRLAAEFRH
                     TFKKDVVVDLWCYRRHGHNEGDEPSFTQPLMYKKIAQHATVRQVYVKRLEKEGVIAPG
                     YGQELLDAFNKQLDAAHDSARSYKSNKVDWLEGAWAGLKASPIDYERGKTSSSLESLR
                     EIGLKMTALPKELNVHPRLKRVIGQRRKAIEEGQGIDWATAEHLAFGTLLCEGFPVRL
                     TGQDVGRGTFSQRHATIYDQSTEEKYHPLMHLREDQGSFEVIDSFLSEEGVLGYEYGY
                     SLADPNCLTLWEAQFGDFANGAQVYFDQFISSGEKKWLRFSGLVCLLPHGMEGQGPEH
                     SSARLERFLQLFAEDNIQVCYPSTPASHFHMLRRQLHRDFRKPLIVMTPKSLLRHKRC
                     VSTLEDMGPGTSFHRVMYDRPPSDADQTIERVVMCSGKVYYDLLAAREEQGLDDKVAL
                     IRLEQLAPFPEDALTEELQRYPATASFVWCQEEPRNQGAWFFVAPRIENLMEAQGIAQ
                     QRLTYAGRKPAAAPATGFHAQHEREQRVLVNEALTG"
     gene            10946..>11522
                     /locus_tag="P5558_15760"
     CDS             10946..>11522
                     /locus_tag="P5558_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRY25824.1"
                     /translation="MSGEIRVPTLGESVSEATIARWLKKAGDTVSVDEPLVELETDKV
                     SLEVPAPVAGVLAEIMAEEGSDVEVGALLGVIEEGAAAPAKKAEAAPAAKAEKAAGSP
                     AEPTEKGDVVDLSGANKSGPAARKIAAEKGLDLAGIEGTGPKGNVTKGDVLKAERPAA
                     APAAKPAAPVASTGREERVRMTRLRKRIAERL"
BASE COUNT         1940 a         3944 c         3915 g         1723 t
ORIGIN      
        1 cgctggctcc ggcagagcac cgccagcacc tgcagggcgg cgagcagccc gtcgccggtg
       61 gtggcgaagt cgctgagcac gatgtggccg gactgctcgc cgccgacatt gaagccgccc
      121 tcgcgcatcc gctcgacgac atagcggtcg ccgaccctgg tgcggtggag gtcgaggccg
      181 aggcccctga ggaagccctc gaggccgaaa ttggacatca gcgtcgcgac cacgccgtcg
      241 ccacgcagcc ggccgtccgc catccagctc tgggcgatca gcgccaggag ctggtcgccg
      301 tcgacgagct ggccctgctc gtcgacgagg atcaggcggt cggcatcgcc gtcgagggcg
      361 atcccgagat cggccttgtg ctcgagcacc gccgccttga tcgtctcggg ataggtggag
      421 ccgcagccgg cattgatgtt gcggccgttc ggctggacgc cgaggccgac gacctccgca
      481 ccgagctcga agaagagctc gggggcgatg tggtaggcgg acccgttggc gcaatcgacc
      541 acgatcttgc gcccgcccag ctcggcaccg cgcgggaagc tgctcttcag gttctcgaca
      601 tagcgcccac gggcatcctc gtagcgcatc gcccggccga tctcgtccgg cggtgcctcg
      661 ccgtcggccg ccatggcctc catccgcgcc tcgatctcgg cctcgagcgc atcggagagc
      721 ttgaagccat ccgggccgaa gagcttgatg ccgttgtcct cgtagggatt gtgcgaggcc
      781 gagaccatga ccccgagatc ggccctgagc gagcgggtaa ggaaggccac ggccggcgtc
      841 ggcaccggac ctgtcagcat cacccgcatg ccggcggaga gaaatcccgc cgtcagcgcg
      901 ttctcgatca tgtagccgga aagccgcgtg tccttaccga tgacaacgcg gttctcgtga
      961 cggcggccgt gctggccggc gcgcctgaac aggccgcccg cggcacgacc cagcgccagg
     1021 gccacctcgg cggtcatcgg gtgggtgttg ctggtgccgc gaataccgtc cgttccgaac
     1081 atccggcgtt gcatcgatca gtcgtcctcg tgctgcggag caatggcagg tcgaagggcc
     1141 gatccgggaa gctggtcgcg cagggttaag cccccgacgc ctccgggtag gtgctcgacg
     1201 aattcggccg ttcggccggc attctctcgg actaagctcc gtccaggtca acctttcccg
     1261 cttgcgcaat catggcagcc gcccgcgaca cccccttcat gccggtcgag atcatccggc
     1321 cgagcggcca agtcgagcga cccgagcgcc ggcagggggc agcagggttt cgaccctacg
     1381 tggcgggtga cttcttgccg cccggctcgc cgcccaatcc cgtcgagcga cgcgagcggc
     1441 gccggtcgcg cggcgccgac cagcgcggcc gccggatcga tatcgtctcc tgacgggacg
     1501 gctgcgaccg gcgcccgcct cgccggctcg gctcccgccg acccgctctg gctcggcctg
     1561 ttcctcgggt tcggccggtc gcctcaagtt cgaccggcaa gggtctcgaa catgacgatg
     1621 cagccgcgcc gcgccggatc gaccgacggc gcggagaggg ccacggcgat cgcctgggcg
     1681 gtgcgcacct tgcccggcga gacataggtc gcgttgaagc cggcccgggc catccgcccg
     1741 tcgagcaggc tcgtccacat gtcgcgcatg ccggggccgc gcaggatcat gatgttgtcg
     1801 aggcgctggc ccacggtcgc cttggggtcg agattgagcc agaaggcgaa ggcgcggttc
     1861 atctggcgga tcgcccggtc gccgtcgatg atcagggacg gcagcgggca cgcttccagc
     1921 acccggacga gatcctgggt gttgccggcg agcgccagct tgtccacgtc ccgctgcatc
     1981 gtctcggtgc gcagccgggc atgcaccagc gcataggtgc gcagcgtcga ggcgagattt
     2041 gtccgctcgg gcgtcgccga tgcgacctcg ttgacgaggc cggaaagccg cagatgccgc
     2101 tgctgtgcca cggccttgag gtattcccag aactcgcttt ccagcttgat cgaggttcgg
     2161 acctcaccga tccgcatggt cttgcggatc atgtggcaca gttccaacgc gcagcgtgca
     2221 tacgcaccat gatattcacc tcagcgccta ttctcgattg agaagagtaa atgcgctcta
     2281 taggttaggt tttggttaac gcgaatgaca aaaagccgaa ctcgcctacg acgcaggagc
     2341 gcaacatgac cgaacggact gggtcggact ggccagtcga cgggttggaa ccgattgtgg
     2401 tcgcgcaggc cgaatccgcc caggaagcca gcggccagcc acgtgtgctg gtccaggagc
     2461 cggccgccgt cgaggaggtc gaggcggtcg ccgcccctgc gtcgacgccg gtcgacccgg
     2521 aaaccctcgg catcgccccg gtcgacgcgg gcgaacgacg ggtcatcgcc gtgacacccg
     2581 gcacgaccgt ggtcctcgac gacccggcct tcgatccgtc ccgtgccgtc tatgcggtcg
     2641 acgggctcga cctcgtggtc acccagccca acggcggggt cctggtgctc gtcggcttct
     2701 tcgcgccgag cgaggcgccg ccggagctca gcgtgctcgg cgcgccggcg acctcggccc
     2761 ctgtcctcct gcagcaggcc gaggcggcac cggtgctcga cgaccagagc ggcgtcgagc
     2821 cggcggccgg accggtgacc ggaccgcagg gcggcggcgg cgcgggcttc acggcttacg
     2881 acgcgggctc gatcggcgaa ggcctcgacc cgctcgggcc actcgggccg acctcgctcc
     2941 tctacagcgc ccccgatccg gaggtcggca acaccttcgt cggcgacgaa gatggcaacg
     3001 gcggcatcaa cccgccgccg cccaccgggc cggtggtgac catgcagtcg aatatctcgg
     3061 cgaccgtcgg agaggtatcg ggaccgggct tcaacccgaa gacgacgccc gtcctgccct
     3121 tgagccagag cgcgctgacg ccggacggcg tcgatcgcga ggggctcagc taccagaagt
     3181 ggctggcgag caagggccct atcgatccgc tcgccctcga cagcatcaac agcgtcgacg
     3241 agggcaacgt cacgctcgat atcgagcgcg aggtcttcat ccgcttcgtc aacgagaact
     3301 cgtactcggt ggacagcgtg ttcgttcacg acatcgcccc cgacgggtcg atcgtgaacg
     3361 tgcggctggc cttcgacggc accaatgtcc cccaggatgc ctttgccgag ctcaagaccg
     3421 tgcagcccgg aacggagatc tccctcggca ggttcgcagc cggcacaccc atcggcattc
     3481 tctttctcaa cgacgggttc cgcgagaacg acttcgccaa attgcagggc ggccggtacg
     3541 aggtgcgcga tccgctgacc ggcgagatcg ccaagatcac ggatgttcat cccaccgaca
     3601 agccccagct caatccactg ctcgtgcaca ttgccgacga cggcacggaa accgtgctca
     3661 aggggcagga cgggaaccgc atcttctcgg ccgacgcgac tcccgcgtcg cccaatcaga
     3721 acgtgatcaa tcccgacggg ctcggccatt tcgtttcggg ctgggacgcc ggcaacgggc
     3781 tcctgctcgc cggtgtcgac gacggcatcg ggacgttcag ggatctcagc ttcgacgatc
     3841 tggttattgg cgtgcgcttc ggctcacccc tgaacaacac gctggtcatc gaggccttcg
     3901 agaacggtgt ggacatccag atcaccgacg cggacagcac ggagatggcc tcggccacca
     3961 tcgcgctgaa cgggttcgcg ggcgacaccc tgctgctcga tcccagcgtc gcggccggca
     4021 ccggcatcac cgtgacgcag gtcagcgaca cgctgatcga gctccagggg ctcgcctcga
     4081 tcgacgccta ccagcaggtg atcaatgccg catcgatcgg catcgacctc cagaaccccg
     4141 agttcggcga gcggcagatc gaggtcggcg tgaccgaccc cgatggcctg accggcacgg
     4201 gcacgaccac catcgacgtc cagaacaacc ttctgaccgg gcccggcgac ggcagtggca
     4261 acgacacgat catcggcaca gcacccggtg acggccagtc cggcgacgac gtcatcagcg
     4321 ggcggggtgg cgacgacacc atcttcggca tgggcggcaa cgacttcgtc gatggcggcg
     4381 acggcctcga cacgatcgac gtgagccagc ccggcatgaa cacggtcatc ggcgggccgc
     4441 agccggaccg gatcgttctc ggccccggcg ccgatgtcgt gaggattacc ggcctctccg
     4501 acggtgccga cacgatcagc ttcttcaatg cctcggaggg cgacaagctg gacctcgtgg
     4561 agctgtttcg ggacagcgac atcaccgccg gcaacatcgc ggactatgtc cagaccggcg
     4621 ccatctccgg cggcatcagc gtgcaggtcg acctggatgg ccggggcggc gacgccagat
     4681 tcgtggacat cgcctatctg cagaacacga ccggcgtcgt ggccggcggg gatccgtcga
     4741 gcttcgtgat catccccggc gccgagccgc cggtgagctg aggccgaccg ggcaatcgcg
     4801 agagggccgg ccctggtgcc ggcccttttc ctttccggcc ggatgcccgc ggatccggcg
     4861 ggcgtcgcgc tgttgcgccg cagcaaggcc atgccggtgc gacgccggtc gccttgaaga
     4921 gccgccccaa ggtcggtaga caggcggcgg cttgcgatgg gcatctggct gaatagggag
     4981 cgaaaccgca tggctcgcag gaagatcgca ctggtcgggg ccggccagat cggcggcacg
     5041 ctggcgttgc tcgccgggct caaggatctg ggcgacgtcg tcctcttcga tgtcgccgag
     5101 ggcatgccgg ccggcaaggc gctcgatctg gtgcagagct cgccggtcga gggcttcgac
     5161 gcgcggctca ccggcaccaa cgactatgcc ggcatcgccg gcgccgacgt ggtcatcgtg
     5221 acggcgggca tcccgcgcaa gcccggcatg agccgcgacg acctcatcgc caccaatgcc
     5281 ggcgtcatca agaccgtggg cgaagcgatc aagcagcatt gcccggatgc cttcgtgatc
     5341 gtcatcacca acccgctcga tgcgatggtc tgggtcatgc agaaggtgag cggtctgccc
     5401 gccgcccgtg tggtgggcat ggccggcgtg ctcgacagcg cgcgctttcg ctggttcctc
     5461 gccgaggaat tcgacgtctc ggtcgaggac gtgacggcct tcgtgctggg tggccacggc
     5521 gacagcatgg tgccgctggt gcgctactcg acggtcgccg gcattccgtt gcccgacctg
     5581 gtgaagatgg gctggacgac gcaggataag ctcgacgcca tcgtccagcg gacccgtgac
     5641 ggtggtgccg agatcgtcgg cctcctgaag accggctccg ccttctatgc gccggccgcc
     5701 tcggcgatcg ccatggccga gagctttctc aaggacaaga agcgcgtgct gccttgcgcc
     5761 gcacagctca gcgggcaata cggcgtcgac ggcctctatg tcggcgtgcc ggtggtgatc
     5821 ggggcgggcg gcgtcgagcg catcgtcgag atcgagctcg acccggccga gcgggccgcc
     5881 ttcgacaagt ccgtggccgc cgtcaagggc ctctgcgacg ccgtccagct ctaggagctt
     5941 ctgcgatgaa catccacgaa taccaggcca aggcgctgct cgccgattac ggcgtggcga
     6001 ccctcgccgg ccgcgtggcc tacacgcccg aggaagccga gcaggcggcg cgggccctcg
     6061 gcgggcccgt ctgggtggtc aaggcgcaga tccacgccgg cggccggggc aagggcggcg
     6121 gcgtcaagct ggcgcgctcg gtcgaggagg tccgccagct cgccgaccag atcctcggca
     6181 tgaccctggt cacccatcag acggggccgc agggccgcgt ggtcaagcgc gtctatatcg
     6241 aggacggctg cgacatcgcc cgcgagctct atctgggcgc ggtgatcgac cgggcgtcgt
     6301 ccaggatcac cttcatggcc tcgaccgagg gcggcgtcga gatcgaggag gtggcggcga
     6361 cccatccgga gaagatcatc aaggtggcga tcgacccggt caccggcttc atgccgttcc
     6421 acggccgcca gctcgccttc ggcctcggcc tcgagggtgc cgccgtccgg aaggccgtgg
     6481 cgttcatgca ggggctctac aactgcatcg tcgagcgcga cgccagcctg gtggagatca
     6541 acccgctggt ggtcaccggc gccggcgaca tcgtggcgct cgatgccaag atgaacttcg
     6601 acagcaacgc cctctaccgc cagcccaagg tggtcgatct gcgggacctc gacgaggagg
     6661 acccgcacga gatcgaggcc agccggcacg agctgaacta catcaagctg gacggcaaca
     6721 tcgcctgcat ggtcaacggc gccggtctcg ccatggcgac catggacatc atcaagctct
     6781 tcggcggcga gcccgcgaac ttcctcgacg tcggcggcgg cgccaccaag gagcgggtga
     6841 ccgcggcctt caagctcctg ctctccgacc cgaatgtcga gggcgtgctg gtcaacatct
     6901 tcggcggcat catgcgctgc gacgtcatcg ccgagggcgt cgttgccgct gcccgcgagg
     6961 tcaatctcaa cgtcccgctc gtggtccggc tggaagggac caatgtcgag ctcggcaagc
     7021 agatcctcgc cgattcgggc ctttcgataa ccccggccga cgatctggcg gatgccgccg
     7081 agaagatcgt ggccgccgtc aggggggtca gctgatggcc gtgctcatcg acgcctcgac
     7141 caaggtcatc tgccagggct tcaccggcgc ccagggcacc ttccattccg agcaggcgat
     7201 cgcctacggc acgcggatgg tcggcggggt cacgcccggc aagggcggcg agaagcatct
     7261 gggcctgccg gtcttcgata ccgtcaaggc ggcggccgaa ggcaccggcg ccgatgcgag
     7321 cgtgatctac gtcccggccc ccttcgcggc ggatgcgatc ctcgaggcga tcgaggccgg
     7381 catcgggctg gtcgtctgca tcaccgaagg catcccggtg ctcgacatgg tcaaggtccg
     7441 ccgcgccctc acgggctcga cgacccggct ggtcggcccc aactgtccgg gcgtcatcac
     7501 gcccggcggc tgcaagatcg gcatcatgcc gggccatatc cacaagcccg gcagcatcgg
     7561 catcgtctcg cgctcgggca ccctcaccta tgaggcggtg gcgcagacca cggccgccgg
     7621 gctcggccag tcgacctgcg tcggcatcgg cggcgacccg gtcaagggga tggagttcat
     7681 cgacgtgctc gagctcttcc tcgccgacga cgcgacccag ggcatcatca tgatcggcga
     7741 gatcggcggc accgccgagg aggacgcggc cgagttcctc cgctcgcagc ccgtgaagaa
     7801 gccggtcgcc gggttcatcg ccggccgcac cgcaccgccc gggcgccgga tgggccatgc
     7861 cggcgccatc atctccggcg gcaagggcgg ggccgaggag aagctcgagg ccatgcgcgc
     7921 tgccggcatc gtgatcgcgg aaaatccggc cggcatcggc aaggccatgg tggaggcgct
     7981 cggccgggcc taagaacctg tccgagaggt cgggctccgg gggctgggcg gaccggcgat
     8041 cgaggggtgg cacgagggct atggcaacgc gcaaagagct ggagcagacg tcctttctct
     8101 acggtggcaa ctcgagcttc atcgaggagc tctatgcccg ctacctgaac gatccggcga
     8161 gcgtcgagcc gagctggcgc ggctatttcg acgggctcga cggcgaggat cgcgcccttt
     8221 tcggccgggc gcgggccgcg ctgcagccac gccccaggag cctggccgag ccggccaacc
     8281 tcaaccggcc gatctcggcc ggcctcgagt cggatgccac caagaccctg atccgcgagc
     8341 atctgcggct gatcatgctg atccgggcct atcgcgtgcg cggccacctg atcgcgaagc
     8401 tcgaccccct caagatcagc cacaaggcgc accaccccga gctcgactac cattcctacg
     8461 gcttcaccga cgccgatctg gatcgcgagt tctatctcga cttcgtgctc ggcctggaga
     8521 cggcgacgct gcggcaggtc atggacgtgc tgcagaagac ctacagctcg accgtcggca
     8581 tcgagttcct gcacatccag gaccccgacc agaagagctg gctgcagcag cggatggaga
     8641 gtgcccaggg cctcttcgac acgacgcccg aggaaaagcg cgagatcctc gagcacctga
     8701 ccgagaccga gggcttcgag cagttcctgc atgtcaagtt cccgggcacc aagcgcttcg
     8761 gcctggacgg tggcgagagt gcgatcgccg cgctcgagac gatgatccgg accagcgccg
     8821 agctcggcgt cgacgagatc gccctcggca tgccgcatcg tggccggctc aatgtgctcg
     8881 ccaacgtcat gggcaagccg tacgcggcga tcttcagcga gttccagggt gcggccagct
     8941 acgacgacgt gctgggctcg ggcgacgtca agtatcacct cggcacgtcg accgaccggg
     9001 tcctgcccga cggccgggag atccatctct cgctcaccgc caatccctcg catctcgagg
     9061 cggtcaaccc ggtggtgatg ggcaaggtca gggcgaagca gcggcagaag aacgacaccg
     9121 agcgcagtcg ggtcctgggc atcctcatgc acggcgacgc ggccttcgcc gggcagggcg
     9181 tcgtccacga atgcctcgag atgtcggagc ttcgcggata ccgggtgggc ggcacgctgc
     9241 acctgatcgt caacaaccag atcggcttca cgacgagccc ggcctatgcc cgcagctcgc
     9301 cctacccctc ggacgtggcg cgcggcatcc aggcgccgat cttccacgtc aacggcgacg
     9361 atccggtggc ggtgacccat gtctcgcggc tggccgccga gttccgccac accttcaaga
     9421 aggacgtggt cgtcgatctc tggtgctacc ggcgccacgg gcacaacgag ggcgacgagc
     9481 cgagcttcac ccagcccttg atgtacaaga agatcgcgca gcacgcgacg gtgcggcagg
     9541 tctatgtcaa gcggctcgag aaggagggag tgatcgctcc gggctacggg caggagctgc
     9601 tcgacgcctt caacaagcag ctcgacgcgg cgcacgactc ggcgcggagc tacaagtcga
     9661 acaaggtcga ctggctggag ggcgcctggg ccggtctcaa ggcctcgccc atcgactacg
     9721 agcgcggcaa gacatccagc tcgctcgaga gcttgcggga aatcggcctg aagatgaccg
     9781 cgctgcccaa ggagctgaac gtccatccgc ggctcaagcg ggtgatcggg cagcgccgca
     9841 aggccatcga ggaaggccag ggcatcgact gggcgaccgc cgagcatctc gccttcggca
     9901 cgctgctctg cgagggcttc cccgtccggc tgaccggcca ggatgtcggc cgcggcacct
     9961 tctcgcagcg ccacgccacc atctacgacc agtcgacgga agaaaaatac catccgctga
    10021 tgcatctgcg cgaggaccag ggcagcttcg aggtgatcga cagcttcctc tccgaggagg
    10081 gtgtcctggg ctacgagtac ggctactcgc tcgccgatcc gaattgcctc acactctggg
    10141 aggcgcagtt cggcgacttc gccaatggcg cgcaggtcta tttcgatcag ttcatctcct
    10201 cgggcgagaa gaagtggctg cgcttctcgg ggctcgtctg cctcctgccg cacggcatgg
    10261 aaggccaggg accggagcac agctcggcgc gcctcgagcg gttcctgcag ctcttcgccg
    10321 aggacaatat ccaggtctgc tacccctcga ccccggccag ccacttccac atgctgcgcc
    10381 gccagttgca tcgcgacttc aggaagccgc tgatcgtgat gacgccgaag tcgctcctgc
    10441 gtcacaagcg ctgcgtctcg acgctcgaag acatggggcc gggcacgagc ttccaccggg
    10501 tcatgtacga ccgcccgccg agcgacgccg accagacgat cgagcgggtc gtgatgtgct
    10561 cgggcaaggt ctattacgat ctgctcgcgg cgcgcgagga gcaggggctc gacgacaagg
    10621 tggcgctgat ccggctcgag cagctggccc ccttccccga ggacgcgctg accgaggagc
    10681 tgcagcgcta tccggccacg gcgagcttcg tctggtgcca ggaggagccg cgcaaccagg
    10741 gtgcctggtt cttcgtggca cccaggatcg agaatctgat ggaggcgcag ggcatcgccc
    10801 agcagcgcct gacctatgcc ggccgcaagc cggccgccgc cccggcaacg gggttccatg
    10861 cgcagcatga gcgcgagcag cgagtcctcg tcaacgaagc gttgaccggc tgagcggcca
    10921 cacggatgag acgggaggag tcctgatgag cggtgagatc cgggtgccca ctctgggtga
    10981 atcggtgagc gaggcgacca ttgcccgctg gctgaagaag gccggcgaca ccgtttctgt
    11041 cgacgagccc ctggtcgagc tcgagaccga caaggtcagc ctcgaggtgc ccgcccccgt
    11101 ggccggcgtg ctcgccgaaa tcatggccga ggagggcagc gacgtcgagg tcggcgccct
    11161 cctcggggtg atcgaggaag gtgccgccgc accggccaag aaggccgaag cggcgccggc
    11221 ggcaaaggcc gagaaggccg ccgggagccc ggccgagccg accgaaaagg gcgacgtggt
    11281 cgacctgtcc ggggccaaca agtccgggcc agccgcgcgc aagatcgccg ccgagaaggg
    11341 cctcgatctc gccgggatcg agggcaccgg ccccaagggc aacgtcacca agggcgacgt
    11401 gctgaaggcc gagaggccgg cggcagcacc ggccgcgaag cccgcggcac ccgtcgcatc
    11461 gaccggacgc gaggagcggg tccgcatgac ccggctcagg aagcgcatcg ccgagcggct
    11521 ga
//
LOCUS       DAOTFI010000160        11514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6673_length_11514_cov_6.805306, whole genome shotgun sequence.
ACCESSION   DAOTFI010000160 DAOTFI010000000
VERSION     DAOTFI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6673_length_11514_cov_6.805306"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="P5558_15765"
     CDS             <1..545
                     /locus_tag="P5558_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25825.1"
                     /translation="AFAVFDFPGRTVLFVCVLASFMMPFEAMVLPLYHLVRFLGWTDS
                     YKALIMPEVASGFIIFLFRQFFVGIPKELFEAARIDGATWWQAYWRIAMPLTGPTIAT
                     ASLMLFILQWEAFFWPLVAASAPDYVVVQVAIARHSNLETTDWGGMFAASSIAVLMAL
                     IPFLAFQRYYVRTIVQSGIK"
     gene            805..1383
                     /locus_tag="P5558_15770"
     CDS             805..1383
                     /locus_tag="P5558_15770"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY25826.1"
                     /translation="MARSVLAMAVVVAVAAWAAPLDGSAQQRDFGPQDWWGPGSGDWE
                     RMWPEGRERDRWSPGRMGVTQNQRMLRHWTFMNGQVPEAYEGATSTVERTPETIAQGA
                     ELYAAHCARCHGEIGYGDGTEGRSLVPSPALLAFLVQRPIAGDAYLNWAIAEGGSAFG
                     TEMPAYKEALAEDDIWAIIAYMRSGFPDTAAR"
     gene            complement(1795..3450)
                     /locus_tag="P5558_15775"
     CDS             complement(1795..3450)
                     /locus_tag="P5558_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY25827.1"
                     /translation="MSLDPVTLAVIQSGLVQVCNEMDIAFSRAAFSPVIAEANDRSDG
                     LYGWADGALIAQGELGLPVFVGTMQDSAKAVIGKIAAGETAAPEPEDVYIVNDPYLGG
                     THLMDVRFVKPFFHRGEPFCWLANTGHWPDIGGAVPGGFSASATEIEQEGLRLPPVKL
                     FKRGRMDPEIYAIIMSNIRVADQRIGDIRAQAAALEVGARRLTALLDRYGRGVVEEAI
                     VLLREAASRQMRAKIAALPEGSWRSTAWVDSDGVVDEPLAIQLRVTKAGDRLVFDFAG
                     SSPPCQGPMNSVIATTRSSVYLAVKHIWPEVPINAGLFEPLEIVDPQGTFLHAEYPRP
                     VSGCAAEVSQRIAEAVFAALVQAVPERVTAAPAGTSGNFALGGFDPERRRSYVMYHIT
                     GGGYGGNARTDGLAYGCSTIGISKSAPLEVMEQQYPILFEEYALREGSGGAGRQRGGM
                     GLRYAVTLRRGTANASFVMDHGRFGPQGVLGGEDGARNRIEIAYQDGRRETPKHLSKA
                     QNIRIAAGDTIRIETPGGGGYGDPAKRDPKLVARDRRRGYLVD"
     gene            3489..3968
                     /locus_tag="P5558_15780"
     CDS             3489..3968
                     /locus_tag="P5558_15780"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HRY25828.1"
                     /translation="MATDMQRLYDEDFYAWTQAQARELRRFAEARPNLPLDLPHLALE
                     IRDLGKEQRNAVRSLVRHILVHLLLLRFSPATEPRRGWQAEVAAARADAADRLTATLQ
                     RGLQRELPKLYTQARSLTRLRLEDHGEPTDLPEDCPFRLARVLDESWYPDHEAMLRR"
     gene            complement(3952..6000)
                     /locus_tag="P5558_15785"
     CDS             complement(3952..6000)
                     /locus_tag="P5558_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRY25829.1"
                     /translation="MIRIGVDVGGTFTDLMAVDDQGAISVVKTPTTVANQADGVLAAI
                     TASGYDLASVATLVHGTTTTTNAFLERKVARVGLITTRGFRDVLELGRRTRPNPYGMT
                     GTFEPLIERPYRLEVAERTSAEGEVLVPLDPAEVGERARALMAAGCEAVVIHFLHSYA
                     NPAHELAAEAAVRAVWPNRYVTVGHKILSEYREYERGCTASVNAAVQPVLDRYVARLA
                     RRLRDGGYAHELLIVQGNGGTLPAGMVVEDAVKTVMSGPAAGVIAAAWTARHAGIADV
                     ITYDMGGTSSDVALILGGVPSVSAELELDYAMPVHVPMVDVNTLGAGGGSIARLDEAG
                     LLRVGPESAGADPGPICYGRGGVEPTITDANLLLGRLPADRLLSVDHPVSMADIEAAI
                     LARIGQPLGLSAVDAAAAILRIANDRMAGAIRMASVARGHDPRDFALLAFGGAGPLHA
                     TALARELGIPRVLVPPRPGVTNALGCLVADARHDFVRTLNRPLAGLDMGELRAVALEQ
                     RAEGRAALARETTPIEGETLLVAADMQFRGQTHLIRVALPEAELLDGSLQLEALQERF
                     AEAYFQRFAVRLPEIGAMLVNLVTTLIGHRPALAPEALVAGDARATGLAGARLGERPV
                     WFAGAFRPTPVYDRQKLPLSTTFEGPAIVQQLDATTVIEPGDTVEVDRLGNLLITVGA
                     "
     gene            complement(6018..6728)
                     /locus_tag="P5558_15790"
     CDS             complement(6018..6728)
                     /locus_tag="P5558_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25830.1"
                     /translation="MTALLELAGVSAAYLKARILFDINLVVGEREVVALVGRNGAGKS
                     TTIKAVMGIQVSRTGAVRFAGRDVSRLPAYMIARLGIGYVPEERRVFSDLTILENLEV
                     GRQVARQGAPTWTPELLFEIFPNLAELRHRPAGQTSGGEQQMLAIARTLMGNPRLVLL
                     DEPSEGLAPVIVEQMAAAVGKLKQEGLAVLLSEQNLRFAGSVADRAYVIESGHIRFEG
                     PMRRLLDDAALRRAHLSV"
     gene            complement(6725..7489)
                     /locus_tag="P5558_15795"
     CDS             complement(6725..7489)
                     /locus_tag="P5558_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25831.1"
                     /translation="MTGAVLEADGLAKSFGGVQAVKAMSFRVAPGEIVALIGPNGAGK
                     STCFNMLMGQLRADAGRVRVLGDDVTGWPPRKIWRKGVGRTFQITQILPSFTVRENVQ
                     MALLSFHRRLFDVFGRARLAHVDAAAALLERVGIVDLAERACGLLAYGDLKRVELAVA
                     LANQPKLLLMDEPTAGMAPAERLALMELTSAIARADRVGVLFTEHDMDVVFGHADRIM
                     VMHRGTLIAAGPPGEVRDDPEVRAVYLGSSLDPVAE"
     gene            complement(7486..9354)
                     /locus_tag="P5558_15800"
     CDS             complement(7486..9354)
                     /locus_tag="P5558_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25832.1"
                     /translation="MGFILVQILTGLSSAASLFLVAAGLSLIFGVTRVVNFAHGSFYM
                     LGAYIGLTLVAVFGDLFGRGHLAFWSALLCAALIVGAIGALVEIVILRRIYRSPELFQ
                     LVATFGVILVIQDLTLWGWGPQDLLGPRAPDLRGAIMIMGEPLPRYDLVLIAAGPVVL
                     LLFWLLLNKTRFGILVRAATADREMVGALGVNQAWLFTGVFFLGSFLAGLGGALQLPK
                     GGANLLMDFGVLAAAFVVVVVGGMGSIAGAFLAAVLIGQLQAFGVLFVPQSTLVMMFL
                     VMAVVLVVRPFGLLGRPELVGQGRQMLDDPLRPTGWRFKILALVLVALLVAAPLWLGD
                     YGLVLLIDILILALFATALHAIIGPGGLVSFGHAAYFGGGAYAAALLVKYAGTPMSLN
                     LVLAPPAAALLALAIGWFCVRLSGVYFAMLTLAFAQIVWSVVFQWGTVTGGDDGLLGI
                     WPAPWASDRVAYYWLVLACCTAGILALRHGLHSPFGYALRAGRDSPLRAEAIGIDVRR
                     QQWVAFTLSGAFAGLAGGLYAYAKGSVFPNEMAIARSFDGIIMVFFGGVQQLSGPLAG
                     AAALTWLKDLLFRLEMWRLLLGLAVIAVVLLFPQGLAGFLKARFGRFVGIRPELSS"
     gene            complement(9429..10649)
                     /locus_tag="P5558_15805"
     CDS             complement(9429..10649)
                     /locus_tag="P5558_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25833.1"
                     /translation="MTTRRQVTAGMPRLAAIAALLFTTGSLAAEPIKIGDINSYTRMA
                     QHTEHAKRGMQLAIDEINADGGINGRVLELISRDDAGEPGEAIRVAEELVSREGVDIL
                     TGTTLSNIGLAVASFAAQNKVLYLAAEPLADAVTLQSGNRYTFRLRPSTYMQAAMLAE
                     LAAENPARRWATIAPNYAYGQEAVAAFKAIMSEKRPDIEWVGEQWPALFAIDAGAEVL
                     ALSAAEPDAIFNVTFAADLAKFVREGGDRGLFEDTFVVSLLTGEPEYIDPLEDEAPEG
                     WLVTGYPWDKIEDPAYVEWLDRYQAAYDDYPRIGSIVGYNSMLALGAALEKTGGDTDP
                     EKLVEAMRGLEFETPQGVLTFRPSDHQASLGAWVGYTTVEDGQGTMRDWYYAPGEKYL
                     PPEEMVAKLRPASD"
     gene            complement(10684..>11514)
                     /locus_tag="P5558_15810"
     CDS             complement(10684..>11514)
                     /locus_tag="P5558_15810"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRY25834.1"
                     /translation="GMTFAADGRCVRIPGQPTIPPSARVRSYPVELNLGLVWVWMGDP
                     ALASRELLFDLPEYHEAGWHPVDGEALEVEANYLALADNLCDPAHVAFVHLSTLGNAA
                     HEDVPIETKVEGELIVTTRWTIDAEPIPLFQKFGSFKGNIDRWQIYNYHAPSIAVIDF
                     GGADTGTGAREGRLHDGAIRILACHFICPVDEARSIDYWLHVDNFPSDAATRARMNEQ
                     FALAFAEDKAILEAIQVEETKLGGRQRPLRLGIDKGAVLMRKTVERMIEAERSVMAAE
                     "
BASE COUNT         1900 a         4049 c         3928 g         1637 t
ORIGIN      
        1 tcgccttcgc cgtcttcgat ttccccggcc gcacggtcct tttcgtctgc gtcctcgcct
       61 ccttcatgat gccgttcgag gcgatggtgc tgccgctcta ccatctcgtg cgctttctcg
      121 gctggaccga cagctacaag gcgctgatca tgcccgaggt cgcgagcggc ttcatcatct
      181 tcctcttccg ccagttcttc gtcggcatcc ccaaggagct gttcgaggcc gcccgcatcg
      241 acggcgccac ctggtggcag gcctattggc gcatcgccat gccgctgacc gggccgacca
      301 tcgccacggc atccctcatg ctcttcatcc tccaatggga agcgttcttc tggcccctgg
      361 tcgccgccag cgcgcccgac tacgtcgtcg tacaggtggc gatcgcccgc cacagcaacc
      421 tcgagaccac cgactggggc ggcatgttcg ccgcctcctc gatcgccgtc ttgatggctc
      481 tgatcccgtt cctggccttc caacgctact acgtacgcac catcgtccag agcggcatca
      541 aataggcctc acgccgcgcc tcaccaaaag cggcgcactg ctgccgtcgg gatcgcaagc
      601 acgaatgaag tcatcggcca tgccggcggc cgcgaccaag ggaaaaggag agaccccgga
      661 ccgcacccac attcgagaca cggatcagaa atccggcgcc gagggacatc cccttccaaa
      721 cgccttgccc gccccttgat ccagatcatg gcagccggcg accggacagc ggactgtcga
      781 acgagcagca agcgggaggc ccggatggcg agatcagtgc tggccatggc ggttgtcgtc
      841 gcggtcgcgg cgtgggccgc cccgctcgac ggcagcgcgc agcagcgtga cttcgggccg
      901 caggactggt gggggccggg cagcggggac tgggagcgga tgtggccgga gggccgggag
      961 cgggaccgct ggagcccggg ccggatgggc gtgacccaga accagcgcat gctgcgccac
     1021 tggaccttca tgaacgggca ggtgccggag gcctatgagg gtgccaccag caccgtcgag
     1081 cggacgccgg aaacgatcgc ccaaggtgcc gagctctacg cggcgcactg cgcccgctgc
     1141 catggcgaga tcggctacgg cgacggcacc gagggccgca gcctggtccc ctcgcccgcc
     1201 ctgctggcct tcctcgtcca gaggccgatc gccggcgacg cgtatctgaa ctgggccatc
     1261 gcggaaggtg gttcggcctt cgggaccgag atgccggcct acaaggaagc gctcgccgag
     1321 gacgacatct gggcgatcat cgcctacatg cgatcaggct tcccggacac cgccgcgagg
     1381 tagccggcac ccggcaaagg ggatcgagca ccgccccgat gacgcgtcgg atgcgacacg
     1441 cggcctcggg ttgtcgcgtt atacgcgaca ttccggcccg ggttggcgca tattctgcga
     1501 catcccgccc gaattcgtcg cggattccgc gacagaatcg cggggatgtc gcggaatccg
     1561 cgacactcga cgacgatcga acccgccgat tcgacgaacg gacccacgga atcgacgatc
     1621 gaacccggga tttcgacgaa ggaacccggg ctcccgacga acgaacccgg ggtcccggcc
     1681 cagggattcc gggcgttttt cccgcctccc cggcgacgaa cgaacccgaa aaccacgaat
     1741 tgcccaacga acccgcggct cttgacagag gcgccgaact aggctattat tcccctagtc
     1801 gacgagatag ccgcgccttc ggtcgcgcgc caccagcttc gggtcacgct tcgccgggtc
     1861 gccatagcct cccccgcccg gcgtctcgat gcggatggtg tcgccggccg cgatgcggat
     1921 gttctgcgcc ttggagagat gcttcggggt ctcgcgccgg ccgtcctggt aggcgatctc
     1981 gatgcggttg cgggcaccat cctcgccgcc cagcacaccc tgcgggccga accgcccatg
     2041 gtccatgacg aagctggcat tggcggtgcc gcggcggagc gtgacggcat agcgcagccc
     2101 catcccgccc cgctgccgcc ccgccccgcc cgagccctcg cgcagcgcgt attcctcgaa
     2161 gaggatgggg tattgctgct ccatcacctc gagcggcgcg gacttggaga tgccgatggt
     2221 cgagcagcca taggcgagcc cgtccgtccg ggcattgccg ccatagccgc cgccggtgat
     2281 gtggtacatg acgtagcttc gccggcgctc tggatcgaag ccgccgagcg cgaagttgcc
     2341 cgaggtcccg gccggcgccg ccgtcacccg ctccggcacc gcctggacca gggccgcgaa
     2401 gacggcttcc gcgatacgct gcgacacctc cgcggcacag ccggaaaccg gcctgggata
     2461 ctcggcatgg aggaaggtgc cctgcggatc gacgatctcg agcggctcga acaggccggc
     2521 attgatcggc acctcgggcc agatgtgctt gaccgcgagg tagaccgagc tccgggtcgt
     2581 cgcgatcacc gagttcatcg gcccctggca gggcggcgag ctgccggcga agtcgaagac
     2641 caggcggtcg ccggccttgg tcaccctgag ctggatggcg agcggctcgt ccaccacccc
     2701 gtcggaatcc acccaggccg tgctccgcca gctcccctcc ggcagggccg cgatcttcgc
     2761 ccgcatctgc cgcgaagccg cctcgcgcaa aaggacgatc gcctcctcca ccacgccccg
     2821 gccatagcgg tcgaggaggg cggtgagccg cctcgccccc acctcgaggg ccgccgcctg
     2881 ggcccggata tcgccgatcc gctggtcggc gacccggatg ttcgacatga tgatggcgta
     2941 gatctcgggg tccatccggc cccgcttgaa gagcttgacc ggcggcaggc gcagcccctc
     3001 ctgctcgatc tcggtggcgc tcgccgagaa gccgcccggc acggcaccgc cgatatccgg
     3061 ccagtggccg gtgttggcca gccagcagaa gggctcgccc cgatggaaga agggcttgac
     3121 gaagcgcacg tccatgagat gcgtgccgcc gagatagggg tcgttgacga tgtagacgtc
     3181 ctcgggctcc ggcgccgccg tctcgcccgc ggcgatcttg ccgatcaccg ccttcgcact
     3241 gtcctgcatg gtgccgacga agaccgggag gcccagctcg ccctgggcga tgagcgcccc
     3301 gtccgcccag ccatagagcc cgtccgagcg gtcgttggcc tcggcgatca ccggcgagaa
     3361 ggcggcacgg ctgaaggcga tgtccatctc gttgcagacc tgcacgagcc ccgactggat
     3421 gacggcgagc gtgaccggat cgaggctcat ggcgtgcttt ccctgccggt tgaagctatg
     3481 ataggcgcat ggcgaccgac atgcagcggc tctacgacga ggacttctac gcctggacgc
     3541 aggcccaggc gcgcgaattg cgccgcttcg ccgaagcccg cccgaacctg ccgctcgacc
     3601 tgccgcacct cgccctggag atccgcgatt tgggcaagga gcagcgcaac gcggtccgca
     3661 gcttggtgcg gcacatcctc gtccacttgc tgctgctgcg gttctcgccg gcgaccgaac
     3721 cgcgccgtgg ctggcaggcc gaggtggcgg cagcccgcgc tgacgcggcc gaccgcctga
     3781 cggccactct gcaacgtggc ctgcaacgcg aactgccgaa actctacacg caggcgcgca
     3841 gcctcacccg actcaggctc gaggatcacg gtgagcccac cgatctgccc gaggattgcc
     3901 ccttccggct ggctcgggtg ctggatgaga gctggtatcc cgaccacgag gctatgctcc
     3961 gacggtaatt agaagattgc cgagccggtc gacctcgacc gtgtcgccgg gctcgatcac
     4021 cgtcgtcgcg tcgagctgct ggacgatggc cggcccctcg aacgtggtcg agagcggcag
     4081 cttctggcgg tcgtagactg gtgtcggccg gaaggcgccg gcgaaccaga ccggccgctc
     4141 gccgagccgg gcgcctgcga gaccggtggc ccgcgcgtcg cccgcgacca gcgcctccgg
     4201 ggcgagcgcc gggcggtggc cgatcagcgt ggtcacgagg ttgaccagca tcgccccgat
     4261 ctccggcagg cggacggcga agcgctggaa ataggcctcg gcgaagcgct cctgcagcgc
     4321 ctcgagctgc agcgacccgt ccaggagctc ggcttccggc agcgccaccc ggatcagatg
     4381 cgtctggccc ctgaactgca tgtcagcggc caccagcagc gtctcaccct cgatcggcgt
     4441 cgtctcgcgc gccagggccg cccggccctc ggcccgctgc tcgagcgcca ccgcccgcag
     4501 ctcgcccatg tcgagcccgg ccagcggccg gttcagcgtg cggacgaaat cgtgccgggc
     4561 atccgcgacg aggcagccca gcgcgttggt cacccccggc cggggcggca ccagcacgcg
     4621 cggaatgccg agctcgcgcg ccagggcggt cgcgtgcagc ggcccggcgc cgccgaaggc
     4681 gaggagcgcg aagtcacgcg gatcgtggcc ccgcgccacc gaagccatgc ggatcgcccc
     4741 ggccatgcgg tcgttggcga tcctgaggat cgcggccgcc gcgtcgaccg ccgacaggcc
     4801 gagcggctgg ccgatccgcg ccaggatcgc cgcctcgata tccgccatcg agaccgggtg
     4861 atcgaccgaa agcagccggt ccgccggcag ccggccgagc aggagattgg cgtcggtgat
     4921 ggtcggctcc accccacccc ggccatagca gatcggcccc ggatcggcgc cggcgctctc
     4981 cgggcccacc cggagcaggc cggcctcgtc gagccgcgcg atcgacccgc caccggcgcc
     5041 cagcgtgttg acgtcgacca tcggcacatg cacgggcatg gcgtagtcga gctcgagctc
     5101 ggcgctgacg ctcggcacgc cgcccaggat cagcgccacg tccgagctgg tgccgcccat
     5161 gtcgtaggtg atgacgtcgg cgatgccggc atggcgggca gtccaggcgg cggcgatgac
     5221 cccggccgcc gggccggaca tgaccgtctt caccgcatcc tcgacaacca ttccggccgg
     5281 cagggtgccg ccattgccct ggacgatcag cagctcgtgc gcgtagccgc cgtcccggag
     5341 ccgccgggcc agccgcgcga catagcgatc cagcaccggc tggacggcgg cgttgaccga
     5401 ggccgtgcag ccgcgctcgt attcccggta ctccgagagg atcttgtggc cgacggtgac
     5461 gtagcggttg ggccagaccg cccggacggc ggcctcggcg gccagctcgt gcgccggatt
     5521 ggcataggaa tgaaggaaat ggatgacgac cgcctcgcag ccggcggcca tgagcgctcg
     5581 cgcccgctcg cccacctcgg ccggatcgag cggcaccagc acctcgccct cggcactcgt
     5641 ccgctcggcc acctcgagcc ggtagggccg ctcgatcagc ggctcgaacg tgccggtcat
     5701 gccgtaggga ttgggccgcg tgcgccggcc gagctccagc acgtccctga agcccctggt
     5761 cgtgatcagc ccgacccgcg ccaccttgcg ctcgagaaag gcattggtgg tggtggtcgt
     5821 gccgtgcacc agggtcgcca cggatgcgag gtcgtagcca gaggcggtga tcgcggcgag
     5881 cacgccgtcg gcctggttgg ccaccgtggt cggcgtcttg acgacgctga tcgccccctg
     5941 gtcgtccacc gccatgaggt cggtgaaggt gccgccgaca tcgacgccga tccggatcat
     6001 ccgccttggc cctcccttca gacgctcaga tgagcacgac gcagggccgc atcgtcgagc
     6061 aggcgtcgca tcggtccctc gaagcggata tggccggact cgatcacata ggcgcggtcg
     6121 gccaccgacc cggcgaagcg caggttctgc tccgagagca gcaccgcgag cccttcctgc
     6181 ttcagcttgc cgacggcagc cgccatctgc tcgacgatca ccggcgccag gccctccgac
     6241 ggctcgtcga gcagcaccag ccgcgggttg cccatcaagg tgcgggcgat cgccagcatc
     6301 tgctgctcgc cgccgctcgt ctggccggcc gggcgatggc gcaattcggc gaggttgggg
     6361 aagatctcga acaggagctc cggcgtccag gtgggggctc cctgtcgcgc cacctggcgg
     6421 ccgacctcga ggttctcgag gatggtgagg tcggaaaaaa cgcggcgctc ctccggcacg
     6481 taaccgatgc cgagccgggc gatcatgtag gccggcagcc gcgagacgtc ccgcccggcg
     6541 aagcgcacgg cccccgttcg gctgacctgg atgcccatca ccgccttgat ggtggtcgac
     6601 ttgccggcac cgttgcggcc gaccagggcc acgacctcgc gttcgccgac gacgagattg
     6661 atgtcgaaga gaatgcgcgc cttgagatag gcggccgaga cgccggcgag ctcgagcagg
     6721 gccgtcattc cgcaaccggg tccagcgagc tgccgagata gacggcgcgg acctcgggat
     6781 cgtcgcgcac ctctccgggc gggccggcgg cgatgagcgt gccccggtgc atgaccatga
     6841 tccggtcggc gtggccgaag accacgtcca tgtcgtgctc ggtgaagagc acgccgacgc
     6901 gatcggccct ggcgatggcg gaggtcagct ccatcagcgc cagccgctcg gcgggcgcca
     6961 tgccggccgt cggctcgtcc atcagcagca gcttcggctg attggccagc gccaccgcga
     7021 gctcgacccg cttgaggtcg ccgtaggcca ggagcccgca ggcgcgctcg gcgagatcca
     7081 cgatgccgac ccgctcgagc agggcggccg cggcatcgac atgggcgagc cgggcccggc
     7141 cgaagacatc gaagagccgc cggtggaacg agagcagggc catctgcacg ttctcgcgca
     7201 ccgtgaagga cggcaggatc tgggtgatct ggaaggtgcg cccgacgccc ttgcgccaga
     7261 ttttccgtgg cggccagcca gtgacgtcat cgccgagcac ccgcacccga ccggcatccg
     7321 ccctgagctg gcccatcagc atgttgaagc aggtgctctt gcccgcacca ttcgggccga
     7381 tgagcgccac gatctcgccg ggcgccaccc gaaagctcat ggccttcacc gcctgcacgc
     7441 cgccgaagct cttggcgagc ccgtcggcct cgagcactgc cccggtcatg aagagagctc
     7501 cgggcggatg ccgacgaagc ggccgaaacg ggccttgaga aagcccgcga gcccctgcgg
     7561 gaaaagcagg acgacggcga tcacggcgag gcccaggagg agccgccaca tctcgagccg
     7621 gaagaggagg tccttcagcc aggtgagcgc cgccgcgccg gcgagcggcc ccgagagctg
     7681 ctgcaccccg ccgaagaaca ccatgatgat gccgtcgaag gagcgggcga tcgccatctc
     7741 gttcgggaag acgctgccct tggcataggc gtagagcccg ccggcgagcc cggcgaaggc
     7801 accggagagc gtgaaggcga cccattgctg gcggcggacg tcgatgccga tggcctcggc
     7861 ccgaagcggg ctgtcccgac cggcccggag cgcatagccg aaggggctgt gcaggccgtg
     7921 ccgcagcgcc aggatgccgg ccgtgcagca ggcgaggacc agccagtagt aggcgacgcg
     7981 atcgctcgcc cagggcgctg gccagatgcc gagcagcccg tcatcgccac cggtcaccgt
     8041 gccccactga aagacgaccg accagacgat ctgcgcgaag gccagcgtca gcatggcgaa
     8101 gtagacaccg gaaagccgca cgcagaacca gccgatggcg agcgccagga gtgccgccgc
     8161 cggcggggcg aggacgaggt tgagcgacat cggcgtgccg gcatacttga ccagcagcgc
     8221 cgccgcgtag gcgccgccgc cgaaataggc cgcatggccg aagctgacga gcccgcccgg
     8281 cccgatgatg gcatgcaggg ccgtggcgaa gagggcgagg atcaggatgt cgatcaggag
     8341 gaccagcccg taatcgccga gccagagcgg ggcggcgacg agcagggcca cgagcaccag
     8401 ggccaggatt ttgaagcgcc agcccgtcgg cctgagcggg tcgtcgagca tctgccggcc
     8461 ctgcccgacc agctccggcc ggcccagcag gccgaagggc cggacgacca gcaccacggc
     8521 catgacgagg aacatcatga ccagcgtcga ttgcgggacg aagaggacgc cgaaggcctg
     8581 gagctggccg atcagcacgg ccgcgaggaa ggcgccggcg atgctgccca tgccgcccac
     8641 cacgacaacg acgaaggcgg cggccagcac gccgaaatcc atgagcaggt tggcaccacc
     8701 cttcggcagc tgcagggcac caccgaggcc ggcaaggaac gagccgagga agaagacgcc
     8761 ggtgaacagc caggcctggt tgacgccgag ggcacccacc atctcgcggt cggcagtcgc
     8821 agcacggacc agaatgccga agcgggtctt gttgagcagc agccagaata gcagcagcac
     8881 caccgggccg gcggcgatca gcacgaggtc gtagcgcggc agcggctccc ccatgatcat
     8941 gatggccccc cgcaggtccg gcgcccgggg accgagcagg tcctgcggcc cccagcccca
     9001 gagcgtgagg tcctggatca ccaggatgac gccgaaggtg gcgacgagct ggaagagctc
     9061 gggcgagcgg tagatgcgcc gcaggatgac gatctcgacc agggcaccga tggcgccgac
     9121 gataagggcg gcgcagagca gggccgacca gaaggccaga tggccccggc cgaagaggtc
     9181 accgaacacc gccaccaggg tcaggccgat ataggcgccc agcatgtaga acgagccgtg
     9241 cgcgaaattg acgacccggg tcacgccgaa gatcagcgac aggccggccg cgaccaggaa
     9301 caggctggcg gcgctcgaga ggccggtcag gatctgcacg agaatgaagc ccaagtcggc
     9361 agctcccggc gggcgcggtc aaggaagcgg acgggcggtg gcgccgcctg tccgcagagg
     9421 atttcggatc agtcggacgc cggccgcagc ttggccacca tctcctcggg cggcaggtac
     9481 ttctcgcctg gggcatagta ccagtcgcgc atcgtgccct ggccgtcctc gaccgtcgtg
     9541 tagccgaccc aggcaccgag cgacgcctga tggtccgagg gccggaaggt caggacacct
     9601 tgcggcgtct cgaactcgag cccgcgcatg gcctcgacca gcttttccgg atcggtgtcg
     9661 ccgccggtct tctcgagcgc cgcaccgagt gcgagcatcg agttgtagcc gacgatcgag
     9721 ccgatcctcg ggtagtcgtc ataggctgcc tggtagcgat cgagccactc cacataggcc
     9781 gggtcctcga tcttgtccca gggatagccg gtcaccagcc agccctccgg cgcctcgtcc
     9841 tccagcgggt cgatatactc cggctcgccg gtcaagaggc tgaccacgaa ggtgtcctcg
     9901 aagagaccgc gatcgccgcc ctcgcggacg aacttggcta gatcggcagc aaaggtgacg
     9961 ttgaagatcg catcgggctc ggccgccgag agggccagca cctcggcccc ggcatcgatg
    10021 gcgaagagtg ccggccactg ctcgccgacc cattcgatgt ccggccgctt ctcgctcatg
    10081 atcgccttga aggcagcaac cgcctcctgg ccataggcgt agttgggtgc gatcgtcgcc
    10141 cagcgcctgg ccgggttctc ggccgcgagc tcggccagca tcgccgcctg catgtaggtc
    10201 gagggccgca gccggaaggt gtagcggttg ccgctctgca gcgtcacggc gtcggcaaga
    10261 ggctcggccg ccagatagag caccttgttc tgggcggcga aggacgcgac ggcgaggcca
    10321 atattggaaa gcgtcgtacc ggtcaggatg tccacgccct cgcggctcac cagctcctcg
    10381 gcgacgcgga tcgcctcgcc gggctcaccg gcatcgtcgc ggctgatcag ctcgagcacc
    10441 cggccgttga tgccgccgtc ggcgttgatc tcgtcgatcg ccagctgcat cccgcgcttg
    10501 gcgtgctcgg tgtgctgcgc catccgggtg taggagttga tgtcgccgat cttgatcggc
    10561 tcggcagcga ggctgccggt ggtgaagagc agggcggcga tcgccgccag cctgggcatg
    10621 ccggccgtca cctgtcgtcg tgtcgtcatg agccgttctc tcccttggtt ttcctggcgt
    10681 ttctcattcg gccgccatga ccgaccgttc cgcctcgatc atccgctcga ccgtcttgcg
    10741 catcagcacg gcccccttgt cgatgccgag ccggagcggt cgctgccgcc cgccgagctt
    10801 cgtctcctcg acctggatcg cctcgagaat ggccttgtcc tcggcgaagg ccagggcaaa
    10861 ctgctcgttc atccgggccc gcgttgcggc gtcgctcggg aagttgtcga catgcagcca
    10921 gtagtcgatc gagcgcgcct cgtcgaccgg acagatgaaa tgacaggcga ggatgcggat
    10981 ggcaccgtcg tgcaggcggc cctcgcgggc gccggtgccg gtatccgcac cgccgaagtc
    11041 gatcaccgcg atcgagggcg cgtggtagtt gtagatctgc cagcggtcga tattgccctt
    11101 gaagctgccg aacttctgga agagcggaat aggctccgca tcgatcgtcc agcgcgtcgt
    11161 gacgatcaac tcgccctcga ccttggtctc gatcggcacg tcctcgtgcg ccgcgttgcc
    11221 cagcgtcgag agatggacga aggcgacatg cgccggatcg cagagattgt cggcgagggc
    11281 caggtagttg gcctcgacct cgagcgcctc gccgtcgacc ggatgccagc ccgcctcgtg
    11341 gtattccggc agatcgaaga gcagttcccg cgaagcgagc gccggatctc ccatccacac
    11401 ccagacgagc cccaggttca actcgaccgg gtagctgcgc accctggccg agggcgggat
    11461 cgtcggctgg cccgggatgc gcacgcagcg tccgtcggcg gcgaaggtca tgcc
//
LOCUS       DAOTFI010000161        11493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6692_length_11493_cov_7.156408, whole genome shotgun sequence.
ACCESSION   DAOTFI010000161 DAOTFI010000000
VERSION     DAOTFI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11493
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6692_length_11493_cov_7.156408"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..748)
                     /locus_tag="P5558_15815"
     CDS             complement(149..748)
                     /locus_tag="P5558_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRY25835.1"
                     /translation="MKRRSCLAAGAGLAMAGLVLPVPPARATELVEQVLGDPAAPVEI
                     IEYASLTCPHCAAFHTETLPALKERYIDTGKAKLVMRDFPLDQSALFAAALAHCAGPD
                     RYFAFIDALFSSQSRWARAADPKEALLQLAQLGGMPREQAEACLADEALMDAILQVRL
                     DGQNEYAIDSTPSFVVNGKKLTGNHGIDNFAEVIDAAAP"
     gene            complement(829..1269)
                     /locus_tag="P5558_15820"
     CDS             complement(829..1269)
                     /locus_tag="P5558_15820"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRY25836.1"
                     /translation="MTHLLSPAARKRGFAGVKVLEDWPRIVGAGMAKRCQPLRIEFAR
                     GAIAGGTLVLQAAGGAALELQHMGPQLIERINDYFGFRTVRQLRIVQMPLRATPVPAP
                     PARRRLTADEELTLAAGVAAVGDDELGRALAALGRSIMARRPAS"
     gene            1381..2418
                     /locus_tag="P5558_15825"
     CDS             1381..2418
                     /locus_tag="P5558_15825"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRY25837.1"
                     /translation="MPPPPAVLTAAATRLRRTLLEWYDRAKRTLPWRATPGVAADPWA
                     VLVSEIMLQQTTVATVQARFDPFLARFPTPAAMAEAALDDVLHAWQGLGYYRRARGLH
                     ACARTIVHGHAGQVPDESEALAKLPGIGPYTAAAVAAIAFGQQVVPVDGNVARVLARL
                     IAHDRPLSATKAELWPYAAALAGGDRPGDLAQALMELGALVCTPRQPACGICPWAFAC
                     RARAEGRATALPVKVAKRSRPERRAVAMLIRDDRGRLCLRRRPEDGLLGGLIELPAVD
                     PGELPPGDWQALPGTVRHVFTHFSLETTVVTGRPVEALGDAFFHPADRLHELALPTLT
                     RKLLRHAGVAA"
     gene            complement(2415..2777)
                     /locus_tag="P5558_15830"
     CDS             complement(2415..2777)
                     /locus_tag="P5558_15830"
                     /inference="COORDINATES: protein motif:HMM:NF009151.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRY25838.1"
                     /translation="MDRQASERRGRRAETIAAWWLAAKGYRVLARRAATPWGEIDLVC
                     RQGACVVFVEVKARQSVAAALESLGPRQRRRIADAARHYIGRHPPLSALDCRFDLVAI
                     GRFGLPHHLVNAWTPGAF"
     gene            complement(2783..3604)
                     /gene="rsmI"
                     /locus_tag="P5558_15835"
     CDS             complement(2783..3604)
                     /gene="rsmI"
                     /locus_tag="P5558_15835"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRY25839.1"
                     /translation="MATPIGNLGDIGERAKEVLAAADLVLCEDTRHSGRLMAALGIET
                     RLRPYHEHNAARVRPGLIERLAAGGTLALISDAGMPAIADPGHKLVRDAIDAGVLVSA
                     VPGPSAGIMALAISGLPTDRFFFQGFLPAKQVAKRRVLQELAAVPATLVLFESPQRIA
                     GTLGLAAEVLGPRPAAIARELTKLHEEVRRGSLAGLASALAREPVPRGEIVLVIGPPP
                     DAPEVLDGAELDALLEAALRELPPGKAAARVAEATGQPRSLLYQRALALRRPAEG"
     gene            3697..4917
                     /locus_tag="P5558_15840"
     CDS             3697..4917
                     /locus_tag="P5558_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRY25840.1"
                     /translation="MARSRDSLPWLAAVGLILALAGCAKETTGPVYEQPPPVVAEPEP
                     VPAGPVKIALLLPLSGQAASVGQDMLDAAQIALFDVGPTDMTLLPRDTGGTPAGATAA
                     AEDAIAAGAELILGPLFSSSTAAVATVAKEHGIKVVSFSNDTAVASGDVWVLGFRPEE
                     QVARVIGFAREQGLLRIGALAPDDAYGRRAINGFTAALQGSVASLGGPAYAYYPPDER
                     EAARVVREFTGYREPPEGSPPGTSGNTRLFDAILIADGGARLRSIGALLAFYDVDLRT
                     TRPLGTMLWEDDPRLLFEETLQGGWYAGLSPEGERNFQAKFERTFKRKPATLAGLAYD
                     ATALAAVVAASDRTFPRDVLVDPSGFIGRAGIFRLKADGTTDHGLAVLEIDNGTAIVV
                     QPAPDTFLRTPLTQ"
     gene            5240..7090
                     /locus_tag="P5558_15845"
     CDS             5240..7090
                     /locus_tag="P5558_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY25841.1"
                     /translation="MESGDVVPLDTLRGPARLGDHEPADAGIEESLLQSSERPIIRLL
                     IVEDDEEDYDITEELLARAPETRFEVVWASSVEAGLRQIATDRFDACLVDHRLAGRTG
                     LEFAEIAIRRGFKRPIILVSGVGDAELERAALDIGIADYLDKDEFDTGRLDRCIRFAI
                     NRSRNTRRIDPPAATDRLTGVANRTLFLDRVHQALASARRHRLGAGVIALDLDGFRAL
                     NERLGHDAGDRLLGIIAERLGHRLRETDTIARFDADSFAVLIENLQRPEDAALVVQKL
                     MEVIEAPTALAGEVLRPTASFGVALYPIDASAADELLRLAEAAMHRAKQAGGRACRFH
                     DTSLSAVSGRITPSLAGRMVEPAPKGGLDRALQEQRFELHFQPQVTLRSPLVGLAARP
                     RLRDPERGVLGPDRFLAMVEGPQFLPRLTDWMIEAGAAELAAIRAAGCRVDHLAIPIL
                     QPSGEGWAETAERALVALGRHALEPSSLELEFDEATLYEDRTSGLRTIDQLRSRGIRV
                     AVRGYGDAMLSLTLLRDQEIETLKLAQSLLEGVPGDRHRTLFADAVIQLGRHLGVRLV
                     AEEVPGNSQLQMLKRAGCDAAECLMSDSPMTRDDIAPWLLRQQRRRESLL"
     gene            complement(7101..7904)
                     /locus_tag="P5558_15850"
     CDS             complement(7101..7904)
                     /locus_tag="P5558_15850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY25842.1"
                     /translation="MPAGPKPVTCDPSTENDDKSGLLPTALVTGGAVRVGRILALALA
                     ELGFAVAIHCNRATEAAEALAGTIEQEGGRAVVVKGDLAEPGTAAAVVAAAVQGLGEP
                     LGMLVNNAALFVADSLPTLDPSTLRRQLAVNLEAPALLAQSYVAQLPAAAPGLVVNLS
                     DQRVANPTAHYLSYSISKAGLEMLTAVLARSLAPRIRAVNLALGQALPAPGMDPARFA
                     ELVLATPLGRATSAGEIGRALQLIVRSPSMTGTTLTLDSGLATGWLTPS"
     gene            7940..8767
                     /locus_tag="P5558_15855"
     CDS             7940..8767
                     /locus_tag="P5558_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25843.1"
                     /translation="MNLILGGVALYGILVGGLYLLQDSMIFRRDIAAKGSYPLPSGNE
                     RLELRAANGDRLVGNLVRATGRSRGLVIGFSGNAWNADDCTTFLAHRLKDVDIVVFHY
                     RGYTPSEGRPSEAALFEDALLIHDTMVKGMAPERTYAFGFSLGSGVAAYLAAQRPLAG
                     QLLVTPFDSIEAVAASRYMVVPVRYLLKHPFRSTQHLADKAVPTAVILASDDRIVPRE
                     RSERLIEALVDPVMVETIPESTHGGIYDMEMIDGLLRTALDRLEARALANGTVAAAG"
     gene            8777..9331
                     /locus_tag="P5558_15860"
     CDS             8777..9331
                     /locus_tag="P5558_15860"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY25844.1"
                     /translation="MTGIRALLWLLVCWLTLDPARADQDDARLESLFAALQVAGSAEA
                     AQPIEAEIWRIWGETPDPASARLMRQGSRAMGAGAFPIAVLSFNRLVEREPGFAEAWN
                     RRATLHYLMGNYAASIDDIEHTLELEPRHFGALSGLGLIMLGLDRPAAALRSFEAALA
                     VHPYLPGARTHIVELRRRLDGDPI"
     gene            9345..10508
                     /locus_tag="P5558_15865"
     CDS             9345..10508
                     /locus_tag="P5558_15865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HRY25845.1"
                     /translation="MRVLEHERAVTAWVETLAGPDTATLRLGGSWRLAHLAGLDRDLI
                     AALARLLEAPPRRARIVADGLDQLDTAGGWLIVRTIRALEAARVTVELVGARPAHAAL
                     LELLRGSEAAPCDPPPRDHPWLRLVLRTGAGTVHAAQEGARFLSFAGAVTLSLLRQLL
                     RPRTLRLTSIVHHIEQTGVNAIPIIAIMAFFIGIVLAYQGADQLRQFGAEIFTVNLVA
                     VSTLRELGVLLTAIMVAGRSGSAFTAQIGTMKVNEELDAMEVIGLDRLAVLVTPRLVA
                     LAVSLPLLTFLANLMALVGGGLVVVLLLDIPTVLLLRQLQAAVGIDLFVAGMIKAPVF
                     AVLIAAIGCYMGLQVEGNAESVGRLTTQSVVVSIFAVIVVDALFSMLFATFGI"
     gene            10512..11297
                     /locus_tag="P5558_15870"
     CDS             10512..11297
                     /locus_tag="P5558_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY25846.1"
                     /translation="MAEPEHVIPEHVIEVEGLVTTFGATTVHEGLSLRVRRGEIMAVV
                     GGSGTGKSVLVRAITGLLRPKSGSIRLFGEDLGALGDAARQALRARTGMLFQDGALFG
                     SLTVAQNIEVPLSTWHRLRPGLLDELAGLKLALVGLPPAAGSKYPAELSGGMRKRAGL
                     ARALALDPEILFLDEPTAGLDPIAAAAFDELIRSLQAALGLTVVMITHDLDTLQRTAD
                     RIAVLVDKDTTIGTLAELRANPHPWIQAYFAGPRGRAAAGGES"
     gene            11297..>11493
                     /locus_tag="P5558_15875"
     CDS             11297..>11493
                     /locus_tag="P5558_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HRY25847.1"
                     /translation="METRASHLIVGLFVLLLSIGTVLFGVWLAKRDVDQTFTTYEILF
                     EGSVFGLQQGSQVVFSGVPVGR"
BASE COUNT         1567 a         4365 c         3924 g         1637 t
ORIGIN      
        1 tcgagctcgg tcgcatcgca gaacgacttg aagccgctga cgcggagacg cacgaattcc
       61 aaggagcagg tcacaacatc tggtgagggt agccgaactc taccaccaga tgccgtcgat
      121 gcaagcgcct agccgataac gggacccgtc acggcgccgc ggcgtcgatc acctcggcga
      181 agttgtcgat cccgtggttg ccggtcagct tcttgccatt gacgacgaag ctcggcgtcg
      241 agtcgatcgc gtactcgttc tggccgtcca gccgcacctg caggatggcg tccatgagcg
      301 cctcgtcggc gaggcaggcc tcggcctgct cgcgcggcat cccgccgagc tgggcgagct
      361 ggagcagcgc ctccttcgga tccgccgccc gcgcccagcg cgactggctc gagaagagcg
      421 cgtcgatgaa ggcgaagtag cggtccgggc cggcgcagtg cgcgagggcc gcggcgaaga
      481 gcgccgactg gtcgagcggg aagtcgcgca tgaccagctt cgccttgccg gtgtcgatgt
      541 agcgctcctt gagcgccggc agcgtctcgg tgtggaaggc ggcgcaatgc gggcaggtca
      601 gcgacgcata ctcgatgatc tcgacgggtg ccgcgggatc gcccagcacc tgctcgacga
      661 gctccgtcgc ccgcgccggg gggaccggca ggacgagacc ggccatggcg agaccggcac
      721 cggcagcgag acaggaccga cgtttcatcg agagctcccc ttgaacatca tctgggttcg
      781 ggcatgaccc agttatctgg agtggacggc gctgcggaca agacgcgctc agctggccgg
      841 ccggcgggcc atgatgctgc gaccgagcgc ggccagcgcc cggcccagct cgtcatcgcc
      901 caccgcggcg acgcccgccg ccagcgtcag ctcctcgtcc gccgtgaggc gccggcgtgc
      961 cggcggcgcc ggcaccgggg tggcgcgcag cggcatctgg acgatccgga gctggcgcac
     1021 cgtgcgaaag ccgaaatagt cgttgatccg ctcgatcagc tgcggcccca tgtgctgcag
     1081 ctcgagcgcc gccccgccgg ccgcctgcag caccagcgtg ccgccggcga ttgccccccg
     1141 ggcgaactcg atccgcaggg gctggcagcg cttcgccatg ccggcgccga cgatccgcgg
     1201 ccagtcctcg agcaccttga ccccggcgaa gccgcgcttg cgggccgcag gcgacagcag
     1261 atgcgtcaag agccgcgaca gcgccgccgt cgtccgctgc ttctgtcggc tctgggaaac
     1321 cgcccttgtc tgctccacac ttccaggccc gctgtttcgc tgccgaaagc tagcccgtcc
     1381 gtgccgccac cgccagccgt tctcaccgcc gccgcgacac gcctccgccg caccctgctc
     1441 gaatggtatg acagggcgaa gcgcacgctg ccctggcgcg ccactccggg tgtcgcggcc
     1501 gatccctggg ctgtgctggt cagcgagatc atgctgcagc agaccaccgt ggcaacggtg
     1561 caggcccgct tcgacccgtt cctcgcccgc ttcccgacgc cggcggccat ggccgaggcg
     1621 gcgctggacg acgtgctgca cgcctggcag ggcctcggct attaccgccg cgcgcgcggc
     1681 ctgcatgcct gtgcccggac catcgtccat ggccatgctg gccaggtgcc ggacgagtcg
     1741 gaggccctgg ccaagctgcc gggcatcggc ccctacacag cggccgccgt cgccgccatc
     1801 gccttcggcc agcaggtcgt gccggtcgac ggcaatgtcg cccgggtcct cgcccgcctc
     1861 attgcccacg accggccgct ctccgccacc aaggccgagc tctggcccta cgctgctgcc
     1921 ctggcgggcg gcgatcgacc gggcgacctg gcgcaggcgc tgatggagct gggagccctc
     1981 gtctgcaccc cccgccagcc cgcctgcggg atttgcccct gggccttcgc ctgccgggcc
     2041 cgtgccgagg gccgcgccac cgccctgccg gtgaaggtgg cgaagcggtc tcgacccgag
     2101 cgccgggcgg tcgccatgct gatccgggac gaccggggcc ggctctgcct ccgccgccgg
     2161 cccgaggacg gcctgctcgg cgggctgatc gagctgccgg cggtcgaccc gggcgagctg
     2221 cccccgggcg actggcaggc gctcccgggc accgtccgcc acgtcttcac ccatttcagt
     2281 ctcgagacca ccgtcgtcac cggccggccg gtggaggcgc tgggcgatgc cttcttccac
     2341 ccggcggacc gcctgcacga gctggccctg cccacgctca cgcgcaagct gctccgccat
     2401 gccggggtcg ctgcctagaa cgcgcccgga gtccaggcgt tgacgagatg gtgcggcagg
     2461 ccgaagcggc cgatggcgac gaggtcgaag cggcaatcga gcgccgacag cggcggatgg
     2521 cggccgatat agtgccgggc ggcgtcggcg atgcgccgcc gctgccgcgg ccccagcgat
     2581 tcgagcgccg ccgcgaccga ctggcgcgcc ttgacctcga cgaaaacgac gcaggctccc
     2641 tggcggcaga cgaggtcgat ctcgccccag ggcgtcgccg cccgccgggc cagcacccgg
     2701 tagcccttgg ccgccagcca ccaggcggcg atcgtctccg cccggcggcc gcgccgctcg
     2761 ctggcctggc gatccaaatg cctcagccct cggccggccg ccgcagcgcc agagcgcgct
     2821 ggtaaaggag ggagcgcggc tggccggtcg cctcggccac tcgcgccgcc gccttgccgg
     2881 gcggcagctc ccggagcgct gcctcgagca gcgcatccag ctcggccccg tcgagcacct
     2941 ccggcgcatc gggcggcggg ccgatcacca gcacgatctc gcccctgggc accggttccc
     3001 gggccagggc cgaggcgagc cctgccagcg acccccgccg cacctcctcg tgcagcttgg
     3061 tcagctcccg ggcgatggcg gccggccgtg gccccagcac ctcggctgcg agcccgagcg
     3121 tgcccgcgat gcgctgcggt gattcgaaca gcaccagcgt cgccggcacg gcggccagct
     3181 cctgcagcac gcgccgcttc gccacctgct tcgccggcag gaagccctgg aagaagaagc
     3241 ggtcggtcgg caggccggag atggcgagcg ccatgatgcc ggccgacggg ccgggcacgg
     3301 cgctgaccag caccccggca tcgatcgcgt cgcggaccag cttgtggccg ggatcggcga
     3361 tggccggcat gccggcgtcg ctgatcagcg ccagagtccc gccggcggcg agccgctcga
     3421 tcaggcccgg gcgaaccctg gcggcattgt gctcgtgata gggccgcagg cgagtctcga
     3481 tgccgagggc ggccatgagc cggccgctgt ggcgggtgtc ctcgcagagc acgaggtcgg
     3541 cggccgccag cacctccttc gcccgctcgc cgatgtcgcc gagattgccg atcggggtcg
     3601 ccaccagata gaggccagca gcgagtttac tcggacgccg gcctccccta gtttcgtccg
     3661 cgcaggtttc gcgcaatggt tttacggatt cgggcgatgg cgaggtcaag agactccctt
     3721 ccctggctcg ccgcggtcgg tctgatcctg gcgctggccg gctgtgccaa ggaaaccacc
     3781 ggtccggtct acgagcaacc accaccggtg gtggccgagc ccgagcccgt gccggcgggg
     3841 ccggtcaaga tcgccctcct cctgccgctg agcggccagg cggccagcgt cgggcaggac
     3901 atgctcgacg ccgcccagat cgccctcttc gatgtcggac ccaccgacat gacgctcttg
     3961 ccccgcgata ccggcggcac gccggccggg gccaccgccg ccgccgagga cgccatcgcc
     4021 gccggggcgg agctcatcct gggcccgctc ttctcctcct ccaccgccgc ggtcgccaca
     4081 gtggcgaagg agcacgggat caaggtcgtc tccttctcga acgacaccgc ggtcgccagc
     4141 ggggatgtct gggtcctggg ctttcgcccg gaggagcagg tcgcccgcgt catcggcttc
     4201 gcccgcgagc agggcctcct gcgcatcggc gccctcgccc cggacgatgc ctatgggcgc
     4261 cgcgccatca acggcttcac cgcggcgctg caaggctcgg tcgccagcct gggcggcccg
     4321 gcctacgcct actacccgcc ggacgagcgc gaggcggcgc gcgtggtccg cgagttcacc
     4381 ggctaccgcg agccgccgga gggcagcccg cccggcacca gcggcaacac ccgcctcttc
     4441 gatgccatcc tgatcgccga tggcggcgcc aggctgcgct cgatcggcgc cctcctcgcc
     4501 ttctacgacg tggacctgcg gaccacccgc ccgctcggca ccatgctctg ggaggacgac
     4561 ccccggctgc tcttcgagga aaccctgcag ggcggctggt atgccggcct ctcgcccgag
     4621 ggcgagcgca atttccaggc gaagttcgag cgcaccttca agcgcaagcc ggcgacgctg
     4681 gccggtctcg cctatgacgc cacggcgctc gccgccgtgg tcgcggccag cgaccgcacc
     4741 ttcccccgcg acgtgctggt cgaccccagc ggcttcatcg gccgggccgg catcttccgg
     4801 ctcaaggccg acggcaccac cgaccacggc ctcgccgtgc tcgagatcga caacggcacg
     4861 gcgatcgtcg tccagcccgc ccccgacacc ttcctgcgca cgccgctcac ccagtagcgg
     4921 cgcgccgtcg gcacgtcgcg ggtgaaggcc ggcaccggcc ggcccgacac cgggctgccg
     4981 aaaaaattgc cagcctcggc tcgttcaccg accgccggtt gcgtcaacga ggcgacaccg
     5041 ccagacatac cgggctaccg cggtacgcga gcccctcgac aacctgcaag cggcaaacta
     5101 tagcgataat ctatcaatgg tttaaatcgg gctctgaaca gcgctcatga ccacgatggc
     5161 accacgcacc gaccggtttc ccgtcgccag cgacgcgggc accggcagtc ggggctcccg
     5221 accggtggag agcggcccca tggagagcgg cgacgtggtg ccgctcgaca cgctgcgcgg
     5281 cccggcccgc ctcggcgatc acgagccggc cgatgcgggc atcgaggaat cgctgctgca
     5341 gagcagcgag cggccgatca tccgcctcct catcgtcgag gacgacgagg aggactacga
     5401 catcaccgag gagctgctcg cccgcgcgcc cgagacccgc ttcgaggtcg tctgggcgag
     5461 cagcgtcgag gcgggcctca ggcagatcgc cacggaccgg ttcgacgcct gtctcgtcga
     5521 ccaccgcctc gccggccgga ctggcctgga attcgccgag atcgcgatcc gccgcggctt
     5581 caagcggccg atcatcctcg tctccggcgt cggcgacgcg gagctcgagc gggcggcgct
     5641 cgacatcggc atcgccgact atctcgacaa ggacgagttc gacaccggcc ggctggaccg
     5701 ctgcatccgc ttcgccatca accgcagccg caacacgagg cggatcgacc cgccggccgc
     5761 caccgaccgg ctcaccggtg tggccaaccg cacgctcttc ctcgaccgcg tccaccaggc
     5821 gctggcctcc gcccgccggc atcgcctggg tgcgggcgtc atcgccctcg atctcgacgg
     5881 gttccgggcc ctcaacgagc ggctcggcca cgatgccggc gaccggctgc tcggcatcat
     5941 cgccgagcgc ctgggccatc gcctgcgcga gaccgacacc atcgcccgct tcgatgccga
     6001 cagcttcgcc gtcctgatcg agaacctgca gcgccccgag gacgcggccc tcgtcgtgca
     6061 gaagctgatg gaggtgatcg aggcgcccac ggccctggcc ggcgaggtgc tgcgcccgac
     6121 cgcgagcttc ggcgtcgccc tctaccccat cgacgccagc gccgccgacg agctgctccg
     6181 gctcgccgag gcggcgatgc atcgcgccaa gcaggccggc ggccgcgcct gccggttcca
     6241 cgacacgagc ctctccgccg tcagcggccg gatcacgccg tcgctggccg ggcggatggt
     6301 cgagccggcg ccgaaggggg gcctcgatcg tgccctccag gagcagcgct tcgagctcca
     6361 cttccagccg caggtgacgc tccgctcgcc tttggtcggc ctcgccgccc ggccccggct
     6421 gcgcgacccc gagcgcggcg tgctcggccc cgaccgcttc ctcgccatgg tcgaggggcc
     6481 gcagttcctg ccgcggctga ccgactggat gatcgaggcc ggggctgccg agctcgccgc
     6541 catccgggcc gccggctgcc gggtcgacca tctcgccatc cccatcctgc agccgtcggg
     6601 cgagggctgg gccgagactg ccgaaagggc gctcgtcgcc ctcggccgcc acgcgctgga
     6661 gccgtcgagc ctcgagctcg agttcgacga agcgaccctc tacgaggacc gcaccagcgg
     6721 ccttcggacc atcgaccagc ttcgcagccg gggaattcgc gtcgccgtgc gcggctatgg
     6781 cgacgccatg ctctccctca ccctgctgcg cgaccaggag atcgagaccc tgaagctggc
     6841 gcagtccctg ctcgagggcg tgccgggcga ccggcaccgc acactcttcg ccgacgccgt
     6901 catccagctc ggccggcatc tgggcgtgcg gctggtcgcc gaggaggtgc cgggcaacag
     6961 ccagctgcag atgctcaagc gggccggctg cgacgccgcc gaatgcctga tgagcgactc
     7021 gccgatgacc agggacgaca tcgccccctg gctgctgcgc cagcagcgcc gtcgcgaatc
     7081 cctgctttga gcgcagccga tcagctgggc gtcagccagc ccgtggcgag cccgctgtcg
     7141 agcgtgagcg tcgtgccggt catcgagggc gagcgcacga tgagctgcag cgcccggccg
     7201 atctcgcccg cgctggtcgc ccgcccgagc ggcgtggcca ggaccagctc ggcgaagcgc
     7261 gccggatcca tgcccggcgc cggcagggcc tggcccaagg cgagattgac ggcacggatg
     7321 cgcggggcga gcgagcgggc cagcacggcc gtcagcatct cgagccccgc cttgctgatc
     7381 gagtagctca ggtaatgggc cgtcgggttg gcgacgcgct ggtccgagag gttgacgacg
     7441 aggccggggg cggccgccgg cagttgcgcc acataggatt gggcgagcag ggccggggcc
     7501 tcgagattga cggcgagctg ccggcgcagg gtcgaaggat cgagggtcgg cagcgaatcg
     7561 gcgacaaaga gggcggcgtt gttgaccagc atgccgagcg gctcgcccag cccctgcacc
     7621 gccgccgcga ccacggctgc ggcagttccg ggctcggcga ggtcgccctt gaccacgacc
     7681 gcccggccgc cctcctgctc gatggttccg gcgagcgcct cggccgcctc ggtcgcccgg
     7741 ttgcagtgga tcgcaacggc gaagcccagc tcggccaggg ccagggccag gatgcgcccg
     7801 acgcggacgg caccgccggt gaccagggcg gtcggcagga ggccgctctt gtcatcgttt
     7861 tccgtgctgg ggtcgcaggt gactggcttc gggccggccg gcatcctatc tcctccggga
     7921 gtcgagttgg agttggcagg tgaatctgat cctgggcggc gtcgccctct acggcatcct
     7981 cgtcggtggg ctctacctcc tgcaggacag catgatcttc cgccgcgata tcgccgccaa
     8041 gggcagctac cccctgccat ccggcaacga gcggctggag ctgcgcgccg ccaatggcga
     8101 ccggctggtc ggcaatctgg tccgggcgac gggccggagc cgcggcctcg tcatcggctt
     8161 cagcggcaat gcttggaacg cagacgactg caccaccttc ctggcgcatc gcctgaagga
     8221 tgtcgacatc gtcgtcttcc actaccgggg ctacacgccg tccgagggcc ggccgagcga
     8281 ggcagcgctc ttcgaggatg cgctcttgat ccacgacacc atggtcaagg gcatggcgcc
     8341 agagcgcacc tacgccttcg gcttcagcct cggctcgggg gtcgccgcct atctcgcggc
     8401 gcagcgcccg ctcgccggcc agcttctcgt gacccccttc gattcgatcg aggcggtggc
     8461 ggccagccgc tacatggtgg tgccggtgcg ctacctgctc aagcacccgt tccgcagcac
     8521 gcagcacctc gccgacaagg ccgtgccgac tgcggtcatc ctggcgagcg acgaccggat
     8581 cgtgccgcgc gagcgctcgg agcgcctgat cgaggcgctc gtcgacccgg tcatggtcga
     8641 gaccatcccg gagagcacgc atggcggcat ctacgacatg gaaatgatcg atggcctgct
     8701 gcgcacggcc ctcgaccgcc tcgaggcccg ggcactcgcc aacggcacgg tcgccgcggc
     8761 cggctgaggg gcggtggtga ccggcatccg ggcgctgctc tggctcctcg tctgctggct
     8821 gaccctggac cccgcccggg ccgatcagga cgacgcccgg ctcgaatcgc tcttcgccgc
     8881 cctgcaggtg gccggctcgg ccgaggcggc ccagccgatc gaggcggaga tctggcgcat
     8941 ctggggcgag acgccggacc cggccagcgc ccggctgatg cgccagggca gccgggccat
     9001 gggggccggc gcctttccca tcgccgtgct gagcttcaac cggctggtcg agcgcgagcc
     9061 cggcttcgcc gaggcctgga acaggcgggc gacgctgcac tacctcatgg gcaactacgc
     9121 cgcctccatc gacgacatcg agcacacgct ggagctggag ccccggcact tcggcgccct
     9181 ctcgggcctc ggcctcatca tgctcggcct cgatcgcccg gctgccgccc tgcgcagctt
     9241 cgaggcggcg ctcgccgttc acccctatct gcccggcgcc cggacgcata tcgtcgagct
     9301 gcggcgccgg ctcgacgggg acccgatctg aggcggcgcg gcggatgcgg gtgctggagc
     9361 acgagcgggc cgtgacggcc tgggtcgaaa ccctggcggg gccggatacc gcgacgcttc
     9421 gcctcggcgg cagctggcgc ctcgcccatc tcgccggcct ggaccgggac ctgattgccg
     9481 ccctcgcccg cctcctcgag gcgccgccgc ggcgcgcgcg catcgtggcg gacgggctcg
     9541 accagctcga caccgccggc ggctggctga tcgtccgcac catccgcgcc ctcgaggcgg
     9601 ccagggtgac ggtcgagctg gtcggcgccc ggccggcgca cgcggccctc ctcgagctgc
     9661 tgcggggctc cgaggccgca ccctgcgacc cgccgccccg cgaccatccc tggctccggc
     9721 tggtgctgcg caccggtgcc gggacggtgc atgccgccca ggagggggcc cgcttcctct
     9781 ccttcgccgg ggccgtgacc ctgtctttgc tgcgccagct tctccggccc cggaccctgc
     9841 ggctcacctc gatcgtgcat cacatcgagc agaccggcgt caacgccatc ccgatcatcg
     9901 ccatcatggc gttcttcatc ggcatcgtcc tcgcctacca gggcgccgac cagctccgcc
     9961 agttcggggc ggagatcttc accgtcaacc tggtcgccgt ctccacgctc cgcgagctcg
    10021 gcgtgctgct gaccgcgatc atggtggccg gccggtccgg cagcgccttc acggcgcaga
    10081 tcggcacgat gaaggtcaac gaggagctgg atgcgatgga ggtgatcggc ctcgaccgcc
    10141 tcgccgtgct ggtcacgccg cgcctcgtcg ccctcgccgt cagcctgccg ctgctcacct
    10201 tcctcgccaa cctgatggcg ctggtcggcg gcgggctcgt cgtcgtcctc ctcctcgaca
    10261 tccccaccgt gctcctgctg cgccagctcc aggccgcggt cggcatcgac ctcttcgtcg
    10321 ccggcatgat caaggcgccg gtcttcgccg tgctcatcgc cgcgatcggc tgctacatgg
    10381 ggctccaggt cgagggcaat gccgagagcg tcggccggct gaccacccag tccgtggtcg
    10441 tctccatctt cgccgtgatc gtcgtcgacg ccctcttctc gatgctcttc gcgaccttcg
    10501 ggatctgagc cgtggccgag ccggaacacg tcatccccga acacgtgatc gaggtcgagg
    10561 gactggtcac caccttcggt gccaccaccg tccacgaggg cctgtcgctg cgcgtccgcc
    10621 ggggcgagat catggccgtg gtcggcggct cgggcaccgg caagtccgtc ctggtccgcg
    10681 ccatcaccgg cctcctgcgg ccgaaatccg gcagcatccg gctcttcggc gaggatctgg
    10741 gcgccctcgg cgatgcggcc cgacaggcgc tccgggcccg gaccggcatg ctcttccagg
    10801 atggtgctct cttcggctcg ctcaccgtcg cccagaacat cgaggtgccg ctttcgacct
    10861 ggcaccgtct gcggccgggc ctgctcgacg agctcgccgg gctcaagctg gcgctggtcg
    10921 gcctgccgcc ggcagcgggc tcgaaatacc cggccgagct ctccggcggc atgcgcaagc
    10981 gcgccggcct cgcccgtgcc ctggcgctcg atcccgagat cctcttcctc gacgagccga
    11041 ccgccgggct cgatccgatc gccgcggcgg ccttcgacga gctgatccgc tcgctgcagg
    11101 ccgccctcgg gctcaccgtc gtcatgatca cccatgacct cgacaccctg cagcgcaccg
    11161 ccgaccgcat cgccgtcctg gtcgacaagg acaccacgat cggtacgctg gccgagctgc
    11221 gggccaaccc gcacccctgg atccaggcct atttcgccgg cccgcgcggc cgggcggcgg
    11281 ccggtggaga gagctgatgg agacccgggc cagccacctc atcgtcggcc tcttcgtcct
    11341 cctgctcagc atcggcacgg tgctgttcgg cgtctggctc gccaagcgtg acgtcgacca
    11401 gaccttcacc acctacgaga tcctcttcga gggctcggtc ttcggcctgc agcagggcag
    11461 ccaggtggtg ttcagcggcg tgccggtcgg ccg
//
LOCUS       DAOTFI010000162        11358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6785_length_11358_cov_7.058392, whole genome shotgun sequence.
ACCESSION   DAOTFI010000162 DAOTFI010000000
VERSION     DAOTFI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6785_length_11358_cov_7.058392"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..608
                     /gene="yajC"
                     /locus_tag="P5558_15880"
     CDS             225..608
                     /gene="yajC"
                     /locus_tag="P5558_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRY25848.1"
                     /translation="MLISPAYAQAAAPGGSDFFVSLLPLVLIFAVFYFLLIRPQQRKM
                     KDHRALIEGVKRGDQVLTSGGIFGKVTKVEDNVLTVEIAKEVQVSVQRSSIADIVNKP
                     KPGAAEGTAANSNTGGGFLGKLFKK"
     gene            614..2221
                     /gene="secD"
                     /locus_tag="P5558_15885"
     CDS             614..2221
                     /gene="secD"
                     /locus_tag="P5558_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRY25849.1"
                     /translation="MSALQPWKIWTAVVVLLAALVVSIPNLFNRDTVAAWPDFLPKGQ
                     INLGLDLQGGSHLLLEVGVDTVIDERLEELVGEVRLALRRERIGYRGLGVQGGMVRFT
                     LTEPADRDRAVAQLRDLNPLLPSSAFGIGTAREFTVEDDGEGRLAIGFSEEGRAERVR
                     AAVNQSLEVVRRRIDELGTREPTIQRQGEERILVQVPGERDPTAIKNLLGRTARLTFQ
                     MVDMEASIADARAGRLPPGTVLLPSSDPDDGAGAWVVQRRIEVSGENLVDAQPSFQDN
                     QPVVSFRFDNAGARRFGTITQEHTGDLFAIVLDGQVISAPRIREPILGGSGIISGNFT
                     VESANELAILLRAGALPAPLIILEERSVGPDLGADSIAAGRTASIIGIILVAAFMALY
                     YGKFGLFADVALISNIVLILAALTLLGATLTLPGIAGIVLTIGMAVDANVLIFERIRE
                     EARAGRTPLAAIETGFKEAMRTIIDANVTTLIAAVLLFQFGSGPVKGFAVTLAIGILT
                     TLFTATLVTRVIVLFWYRRARPAALPI"
     gene            2235..3203
                     /gene="secF"
                     /locus_tag="P5558_15890"
     CDS             2235..3203
                     /gene="secF"
                     /locus_tag="P5558_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRY25850.1"
                     /translation="MQFRLVPDVTRIRFMRMRHVAMLCSLALAILSIGAVVYPGLNFG
                     IDFKGGILVEVRTPGPADLAQMRTRLNELGLGDVALQEFGEPTDVLIRIARQMGGEEE
                     QQAAVRVVQAALDELVDGEVSYRRTEFVGPKVSEELLRDGVLAIVLSLVAVLIYIWFR
                     FEWQYGVGAIVALIHDVVLTIGMFAVIGFEVNLTTVAAVLTIVGYSLNDTVVIFDRVR
                     ENLRRYKSMPIAELLDVSVNETLARTLMTSLTTLLALLSLYFFGGEVIRGFTFAMIWG
                     VIVGTYSTIFIATPILLQLNLRAIGAKAPGAAAEEIEVGGSPSPSP"
     gene            3203..3598
                     /locus_tag="P5558_15895"
     CDS             3203..3598
                     /locus_tag="P5558_15895"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HRY25851.1"
                     /translation="MAGRPDALQELSTGRLGVTGYRAGGFRIGAQWYNGHVLLLPRGV
                     TAWPVQSAEAIDLDSLLPIREVEPPAEILLIGVGERPLLLPGELRLALKAWGLAVEAM
                     PTSAACRTFNVLLAEERRVVAALLAIEDV"
     gene            complement(3602..4366)
                     /locus_tag="P5558_15900"
     CDS             complement(3602..4366)
                     /locus_tag="P5558_15900"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HRY25852.1"
                     /translation="MASVASTLRGLAVPLVLAGLLAGCAATSTAVSKRNLDVQTKMSS
                     SIFVDPVPADQRTIYIEVRNTSDKPDFTIEPAIRSKVEAAGYRVVDDPTEAHFMLRAN
                     VLQAGRASETAAEAAGGGAFGSAVGGGAIGAGTGYLLGQAGGSDVGLSVAGGLLGAAI
                     GTVADAYVQDVTYTVITDIRVSERAPPSVVVSQSENQSISQGSGGTVTQTSSTISDWR
                     HYESRVVATSNKVNLDWPEAAPDMVDGLTRSIAGIF"
     gene            complement(4427..5032)
                     /locus_tag="P5558_15905"
     CDS             complement(4427..5032)
                     /locus_tag="P5558_15905"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRY25853.1"
                     /translation="MTFTLSDLPYDYEALAPYMSSQTLHLHHDKHHQTYVTNLNNLIE
                     GTDLEGKSLEEIVKASHGDPAKQGIFNNAGQHWNHELFWRVMKKGGGGKPGGELARRI
                     DDAFGNFETFAEQFKTAAATQFGSGWAWLAVSGDTLKVMKTPNGENPLVHGLTPILGI
                     DVWEHAYYLDYQNRRPEYVAAFLNNLVNWEEVEKGMQNALG"
     gene            5182..6000
                     /locus_tag="P5558_15910"
     CDS             5182..6000
                     /locus_tag="P5558_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRY25854.1"
                     /translation="MDTADLLAEARALDPDRTLCVMVGAPADRPLLLALVLLNAELAR
                     IPELVSEPLAGRIRYQWWLDSLAAAAAGRAAHRVPLLAPLAAAFAGGRLDPAAVEALV
                     TAREAELDRLQPENLAALEAYLAASAGALHRLMARACDGSPPEVEAAGDVGIGFALVG
                     LVRALGRERARGRVPLPADLLAAEGVGVDDILAGRAADALDRMSAGLLERAEALLAQA
                     AVLAPFRRRVLPALLPARLARRQARRLRRAPAALPSRDAWTVPDLLWGRLRRRI"
     gene            6134..7159
                     /locus_tag="P5558_15915"
     CDS             6134..7159
                     /locus_tag="P5558_15915"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25855.1"
                     /translation="MHMRITAISTTLLAGLATPFLGGTALAQTDIKFTLDWKYQGPTS
                     AIIAANDLGYFAEEGLTVTIDSGNGSAGAVTRVASGAYQMGFADINALVEFNAANPGQ
                     SVKAVMMVYDAAPFGLYTLKSSGIEKPEDLVGHTLGAPVFDASYKLFPAFAAEVGIDE
                     AAVPRVNMDPPLREAMLVRGDVDVISGHYFSSMLDLMSKGVAEEDITYMLYKDFGMDF
                     YGNAVIASGEFIETHPEAVEGFVRAVARAMQFIISNPEAAVDMVAELDPLIDKALEVR
                     RLGMAIDINIATPYTREHGFGDVDPDRLANSIRQLATALDMANPPSPDEIWTSEFLPP
                     ADQRQLP"
     gene            7183..7965
                     /locus_tag="P5558_15920"
     CDS             7183..7965
                     /locus_tag="P5558_15920"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25856.1"
                     /translation="MSFIRIEDVALRYGGADGTLAVQGCTLAIERGEFAALVGPSGCG
                     KSTLLKMVSGLELPSAGSVRLDGHEVAGPQHGIGIAFQNATLLPWRTTLDNVLLPLEV
                     SREHRGSFRRNKAEHVERARGLLRTVGLDEFREHAPYQLSGGMQQRANLCRALIHEPD
                     LLLLDEPFAALDAFTREELWGVLQQLFMDRRFTVILVTHDLRESVYLADTVHVVSKRP
                     GRIVKTKHIDLPRPRTLEMTFEPGFVDIVHELRDAISEIRGA"
     gene            7962..8723
                     /locus_tag="P5558_15925"
     CDS             7962..8723
                     /locus_tag="P5558_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25857.1"
                     /translation="MTDATKQRLAPWIATAALFLVWELACRVFGVPEFILPTPSASFA
                     ALFEYFDAVMFNASFTLWVTLVGFAIAVVFGLALGVLVGASALTYAALYPILIGFNAI
                     PKVAIVPILVIWFGIGAVPAILTAFLISFFPIVVNVATGIATLEPELKDVLRSLGARP
                     LDILLKVGLPRSMPYFFASLKVAVTLAFVGSIISETIAGNRGIGYLMMQASSSFRVPL
                     VFAGLIVVAVMGVGMYAVFAYVERRMTGWAFRGQA"
     gene            complement(8714..9229)
                     /locus_tag="P5558_15930"
     CDS             complement(8714..9229)
                     /locus_tag="P5558_15930"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HRY25858.1"
                     /translation="MKAVYLAGPEVFFPDVAEVAERLKAACAAAGLLGLFPTDAERPT
                     GAGVSLARAIYLGNVALIERSAAVIADISPFRGAGMDPGTAWEIGFAIARGLPVFAWS
                     LDLRPYAERVGPASPGPRGQVDADGLQIEDFGLVENLMIAESVRAIDATFEAALGRCA
                     KHLLGASTAQA"
     gene            complement(9241..10185)
                     /locus_tag="P5558_15935"
     CDS             complement(9241..10185)
                     /locus_tag="P5558_15935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HRY25859.1"
                     /translation="MLALLALFLWVPGARADGPLDAVLGLRAEVPAAARTAATLGRIR
                     RGSAVLIDDEGLALTIGYLVMEASVVELFDAGARRIPADVVAWDHGTGLGLVRALLPI
                     EAEPLSIAADVASGDPLLVISRMRALDGTQVELADRRVFTGYWEYLLEDALFTSPPHR
                     EFGGAALLDRDGALVGIGSLQVGDALGPGVASPGNMFVPAALLPPILADMLANGHANG
                     ARPWLGITSREDERGVSLVSVSPGGPAERAGLRPGDRVTAVNGEPVTTLEGLYRSVWT
                     AGGPGVAVALTIERGSEEQVIEVRTMDRMAWLRRNPTF"
     gene            complement(10325..10600)
                     /gene="minE"
                     /locus_tag="P5558_15940"
     CDS             complement(10325..10600)
                     /gene="minE"
                     /locus_tag="P5558_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRY25860.1"
                     /translation="MRFFDFFRLNRRDDSANKAKERLTVLVALDRTGGGGPDFLPLLQ
                     RELLEVIRKYVDVDQEKVNVDFQAGTGFSTLEVNVELPNQMAQLRAG"
     gene            complement(10597..>11358)
                     /gene="minD"
                     /locus_tag="P5558_15945"
     CDS             complement(10597..>11358)
                     /gene="minD"
                     /locus_tag="P5558_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRY25861.1"
                     /translation="SAAAIGTGLALRGKKTVIIDFDVGLRNLDLIMGCERRVVFDFIN
                     VINDGIRLNQALIKDKRVDNLFILPTSQTRDKEALAAEGVAKVIEELRKDFDYIICDS
                     PAGIERGAYMALYHADEAIVVTNPEVSSVRDSDRILGILNSKSKRAEHGDEPVKQHLL
                     LTRYDLDRVQRGEMLKLDDVLEILAIPVLGVIPQCGSVLQASNLGMPVVLDDKSKAGE
                     AYKDAVARLLGEDVELRYVTPQKRGLVDWLLRRSA"
BASE COUNT         1685 a         3909 c         3865 g         1899 t
ORIGIN      
        1 tcgtctcgat cggcgacctc gtcaagtcga tcatcgacga gcaggagcac acgatcgcgc
       61 agctggtcca atacgtcacg ggcgctgcct gaggcgcggt ttcagcgtca ccggccgagg
      121 cggcggcgcc cctgccggcg cccgcgcgct tgtggtttgc caagcgggcc ctgccgttat
      181 agaaggcagc cccatccgca ggccctgctc cttcgaggac aagaatgctc atctcaccgg
      241 cctatgccca ggcggcagcc ccgggcggca gcgatttctt cgtgtcgctc ctgccgctgg
      301 tgctcatctt cgccgtcttc tatttcctcc tcatccgccc gcagcagcgc aagatgaagg
      361 atcatcgtgc cctcatcgag ggcgtcaagc gcggtgacca ggtgctgacc tccggtggca
      421 tcttcggtaa ggtcaccaag gtcgaggaca acgtgctcac ggtcgagatc gccaaggagg
      481 tgcaggtgag cgtgcagcgc tcgagcatcg ccgacatcgt caacaaaccc aagcccggcg
      541 ccgccgaggg cactgccgcc aacagcaaca ccggcggcgg ctttctcggc aagctgttca
      601 agaaatagcg tcgatgtcgg cactgcagcc ctggaagatc tggacggccg tcgtggtgct
      661 cctggcggcg ctcgtcgtgt cgattcccaa tctcttcaac cgggacacgg tggctgcctg
      721 gccggatttc ctgccgaagg gtcagatcaa cctggggctc gacctgcagg gcggctcgca
      781 tctgctcctg gaggtcggtg tcgatacggt gatcgacgag cggctcgagg agctggtcgg
      841 cgaggtgcgg ctggcgctgc ggcgcgagcg catcggctat cggggcctcg gcgtccaggg
      901 cggcatggtc cggttcaccc tgaccgagcc ggccgaccgc gaccgggccg tggcgcagct
      961 cagggacctc aacccgttgc tgccgtcgtc ggccttcggg atcggcaccg cgcgtgagtt
     1021 caccgtggag gacgacggcg agggccgctt ggccatcggc ttcagcgagg agggccgggc
     1081 cgagcgcgtg cgcgcggccg tcaaccagtc gctcgaggtg gtgcgccgcc ggatcgacga
     1141 gctcggcacc cgcgagccga cgatccagcg tcagggcgag gaacgcattc tcgtgcaggt
     1201 gccgggcgag cgcgacccga ccgccatcaa gaacctcctg ggccgcaccg cccggctgac
     1261 cttccagatg gtcgacatgg aggcgagcat cgccgatgcc agggccggca ggttgccacc
     1321 tggcaccgtg ctcctgccat cgtccgaccc tgacgatggc gccggcgcct gggtggtgca
     1381 gcggcggatc gaggtgtcgg gcgagaacct ggtcgatgcg cagccgagct tccaggacaa
     1441 ccagccggtc gtcagcttcc gcttcgacaa cgccggggcg aggcggttcg gcacgatcac
     1501 gcaggagcac acgggcgatc tcttcgccat cgtgctcgac ggccaggtca tctccgcacc
     1561 gcgcatccgc gagcccatcc tcggcggctc gggcatcatc tcgggcaact tcacggtcga
     1621 gagcgccaac gagctggcga tcctgctccg cgccggtgcc ttgccggcac cgctcatcat
     1681 tctcgaggaa cgcagcgtcg gccctgatct cggcgccgat tcgatcgccg cgggccgcac
     1741 ggccagcatc atcggcatca tcctcgtggc cgccttcatg gcgctctatt acggcaagtt
     1801 cggtctcttc gccgacgtgg cgctgatcag caacatcgtc ctcatcctgg cagcgctgac
     1861 tctcctgggc gccacgctca cgctgcccgg catcgccggc atcgtcctga cgatcggcat
     1921 ggcggtcgat gcgaatgtgc tgatcttcga gcgcatccgg gaagaggcga gggccggccg
     1981 cacgcccctg gcggcgatcg agaccggctt caaggaggcg atgcggacca ttatcgatgc
     2041 caatgtgacg acgctgatcg ccgcggtgct gctcttccag ttcggctccg gcccggtgaa
     2101 gggattcgcc gtcaccctgg cgatcggcat tctgacaacc ctgttcacgg cgacgttggt
     2161 cacccgcgtg atcgtgctct tctggtaccg gcgcgcgcga cccgcggccc tgccgatctg
     2221 aaggctcccc ttcgatgcag tttcgcctcg tccccgacgt cacgcgcatt cgcttcatgc
     2281 ggatgcgtca cgtcgccatg ctctgctcgc tcgccctggc gattctctcc atcggcgccg
     2341 tcgtctatcc cgggctgaat ttcggcatcg acttcaaggg cggcatcctc gtcgaggtcc
     2401 gcacgcccgg gcccgccgac ctggcgcaga tgcgcacccg cctcaacgag ctcggcctcg
     2461 gcgatgtggc gctgcaggaa ttcggcgagc cgaccgatgt cctgatccgc atcgcccggc
     2521 agatgggcgg cgaggaggag cagcaggccg ccgtccgggt tgtccaggca gcgctggacg
     2581 agctggtcga cggcgaggtt tcctaccgcc gcaccgagtt cgtcggcccc aaggtgagcg
     2641 aggagctgct ccgcgacggc gtgctcgcca tcgtgctctc gctggtggcg gtgctcatct
     2701 atatctggtt ccgcttcgaa tggcagtacg gggtcggggc gatcgtcgcc ctgatccatg
     2761 acgtcgtcct gacgatcggc atgttcgcgg tcatcggctt cgaggtgaat ctgacgacgg
     2821 tcgccgcggt gctgaccatt gtcggctact cgctcaacga cacggtggtc atcttcgacc
     2881 gggtgcgcga gaacctgcgg cgctacaagt ccatgccgat tgccgagctg ctcgacgtca
     2941 gcgtgaacga gacgctggcc cggaccctga tgacctcgct gacgacgctg ctcgccctgc
     3001 tctccctcta tttctttggc ggcgaggtca tcagggggtt caccttcgcg atgatctggg
     3061 gcgtgatcgt cggcacctat tccacgatct tcatcgccac accgatcctg ctgcagctca
     3121 atctccgcgc catcggcgcc aaggccccgg gtgcagccgc cgaggagatc gaggtcggcg
     3181 gcagccccag ccccagcccc tgatggcggg ccggcccgat gccctgcagg agctgtcgac
     3241 cggccggctc ggcgtcaccg gctatcgtgc cggcgggttt cgcatcggcg cccagtggta
     3301 caacggccat gtgctgctgc tgccgcgggg cgtgacggcg tggccggtgc aatcggccga
     3361 ggccatcgat ctcgacagcc tgctgccgat ccgcgaggtc gagccgccgg ccgagatcct
     3421 gctgatcggg gtcggtgagc ggccattgct gttgccgggc gagctgcggc tggcgctgaa
     3481 ggcgtggggc ctcgccgtcg aggccatgcc gacctcggcg gcgtgccgaa cgttcaacgt
     3541 gctgctggcc gaggagcgac gggtggtcgc cgcgctcctc gcgatagagg acgtctgagc
     3601 ctcagaaaat cccggcgatc gagcgggtca ggccgtcgac catgtcgggt gctgcctccg
     3661 gccagtccag gttgaccttg ttcgaggtcg caaccacgcg ggattcgtag tgccgccagt
     3721 cgctgatcgt gctcgaggtc tgggtcacgg tgccgcccga gccttgcgag atcgactggt
     3781 tctccgactg gctgacgacg acgctcggcg gcgcgcgctc ggagacgcgg atgtcggtga
     3841 tcaccgtgta ggtgacgtcc tgcacgtagg cgtcggccac ggttccgatg gcggcaccga
     3901 gcaggccgcc ggcgacggag aggccgacat cgctgccgcc ggcctggccg agcaggtagc
     3961 ctgtgccggc gccgatcgcc ccgccgccga cggcgctgcc gaaagcgccg ccgccggccg
     4021 cctcggcggc cgtctcgctg gcgcggccgg cctgcagcac gttggcccgc agcatgaaat
     4081 gcgcctcggt cggatcatcg acgacgcggt agcccgccgc ttcgaccttg ctgcgaatgg
     4141 ctggctcgat ggtgaaatcg ggcttgtcgg aagtgttgcg gacctcgatg tagatggtcc
     4201 gctggtccgc cggcacgggg tcgacgaaga tcgaggacga catcttcgtc tgcacgtcga
     4261 ggttccgctt ggagacggct gtcgacgtcg ccgcgcaacc ggccagcagg ccggccagaa
     4321 ccaggggcac ggccaggccg cgcagggtgg atgccacaga cgccatcgca gacctcactc
     4381 cttcggaccg ggaacgcccg cttgcgttcc cggcagcaga cgtcgctcag ccgagcgcgt
     4441 tctgcatccc cttctcgacc tcttcccagt tgacgaggtt gttgaggaag gcagcaacgt
     4501 attcgggccg gcgattctgg tagtccagat aatacgcgtg ctcccagaca tcgatgccga
     4561 gaatcggcgt caggccgtgt accagggggt tctcgccgtt cggagtcttc atcaccttca
     4621 gcgtgtcgcc gctcaccgcg agccaggccc aacccgagcc gaactgcgtc gccgccgccg
     4681 tcttgaactg ctcggcgaag gtctcgaaat tgccgaaggc atcgtcgatc cgccgggcga
     4741 gctcgccgcc gggcttgccg ccgccgccct tcttcatgac ccgccagaag agctcgtgat
     4801 tccagtgctg gccggcattg ttgaagatgc cctgcttggc cgggtcgcca tgcgacgcct
     4861 tgacgatctc ctcgagcgac ttgccctcga gatccgtgcc ctcgatcagg ttgttcagat
     4921 tggtgacata ggtctggtgg tgcttgtcgt ggtggagatg cagggtctgc gacgacatat
     4981 agggggccag agcctcgtag tcgtaaggca ggtcggagag cgtgaaggtc atgatttcct
     5041 cacccgttcg tgttggaccg tccgcccacc actgtcgcgc tgtccgaacg gccatgttct
     5101 ctgcgctaca taaggtgtgc gggccgttaa agaaagagcc ccggccgcag ttgcgtcacg
     5161 ggtggcggag agcctgaggc catggacacg gccgatctgc tggcggaggc ccgggccctc
     5221 gaccccgacc ggacactctg cgtcatggtg ggcgcgccgg ccgaccgacc gctgctgctg
     5281 gccctggtgc tgctcaacgc ggagctggca cggattcccg agctggtgag cgagcccctg
     5341 gccggccgga tccgttatca gtggtggctc gacagcctgg ccgcggcggc cgcgggcagg
     5401 gccgcccacc gcgtgccctt gctggcgccg ctggccgccg ccttcgccgg cgggcggctc
     5461 gacccggccg ccgtggaggc gctggtgaca gcgcgcgagg ccgagctcga tcgcctgcag
     5521 ccggagaatc tcgcagccct cgaggcatac ctggcggcca gcgccggggc gctgcatcgt
     5581 ctcatggccc gggcctgcga cggcagcccg cccgaggtcg aggcggcagg cgatgtcggc
     5641 atcggcttcg ccctggtcgg cctcgtgcgc gccctcggtc gggagcgcgc acgcgggcgg
     5701 gtgccgctgc cggccgatct gctggccgcc gagggcgtcg gcgtggatga catcctggcc
     5761 gggcgggccg ccgacgcgct cgaccggatg agcgctggcc tgctggagcg ggccgaggcg
     5821 ctgctggcgc aggcggcggt gctcgcgcca ttccgccgtc gggtcctgcc ggccctgttg
     5881 ccggcacggc tcgcccggcg gcaggcacgg cggctgcgcc gcgctcctgc tgcgctgccg
     5941 tcgcgcgatg catggaccgt gcccgacctt ctctggggtc ggctgcggcg gcgcatctga
     6001 tcgcatttcg tgcttgtcag acgggacgtc ctcgcctttg ctgcgcccaa gcagcaagga
     6061 ctgcgtgcgg gccagaacga ccgatagggc gattgccggc aggccttctg ccggtcaaca
     6121 ggaagcacga ggcatgcaca tgcgcatcac cgcgatctcg accaccctgc tagccggcct
     6181 ggcaacgccg ttcctgggcg gcaccgcgct cgcgcagacg gacatcaagt tcaccctgga
     6241 ctggaaatac caggggccga catcggcgat catcgccgcc aatgacctgg ggtatttcgc
     6301 cgaggagggg ctgaccgtca cgatcgacag cggcaacggc tcggccggtg ccgtcacccg
     6361 cgtcgcctcg ggcgcctacc agatgggctt cgccgacatc aacgcgctgg tcgagttcaa
     6421 cgcggcgaac cccgggcaga gcgtcaaggc cgtgatgatg gtctacgatg cggccccctt
     6481 cggcctctac acgctcaagt cctcgggcat cgagaagccg gaggacctcg tcggccacac
     6541 gctgggggcg ccggtcttcg atgcgtccta caagctcttt ccggctttcg ccgccgaggt
     6601 cggcatcgac gaggctgccg tgccgcgggt caacatggac ccgcccttgc gcgaggcgat
     6661 gctggtgcgc ggggacgtcg atgtcatttc cgggcactat ttctcgtcga tgctggacct
     6721 gatgtcgaag ggggtggccg aggaggacat cacctacatg ctctacaagg acttcggcat
     6781 ggacttctac ggcaacgcgg tgatcgcgtc cggggagttc atcgagaccc acccggaggc
     6841 cgtcgagggc ttcgtgcggg cggttgccag ggccatgcag ttcatcatct cgaaccccga
     6901 agcggcggtc gacatggtgg ccgagctcga tccgctgatc gacaaggcgc tcgaggtgcg
     6961 ccggctcggc atggcgatcg acatcaatat cgccacgccc tacacgcgcg agcacggctt
     7021 cggcgacgtc gaccccgacc ggctcgccaa ctcgatccgg caattggcca cggccctcga
     7081 catggccaac ccgccatccc cggacgagat ctggacgagc gagttcctgc cgccggccga
     7141 ccagcgccag cttccctgac cgaccggccg gccggtcgaa gggtgagctt catcaggatc
     7201 gaggacgtcg cgctccgcta cggcggggcg gacgggactc tggccgtcca gggctgcacg
     7261 ctcgccatcg agcggggcga gttcgcggcc ctggtcggcc cgtcgggctg tggcaagtcg
     7321 accctgctga agatggtttc cggcctggag ctgccgtcgg ccggcagcgt ccggctggat
     7381 ggccacgagg tcgccggccc gcagcacggc atcggcatcg cgttccagaa cgcgacgctg
     7441 ctgccgtggc ggacgacgct ggacaacgtg ctcctgccgc tcgaggtgag ccgcgagcac
     7501 cgggggagct tccggcgcaa caaggccgag catgtcgagc gtgcccgggg cctgctccgc
     7561 accgtcgggc tcgacgagtt ccgcgagcat gcgccctacc agctctcggg cggcatgcag
     7621 cagcgcgcca atctctgccg cgcgctcatt cacgagcccg acctgctgct gctcgacgag
     7681 cccttcgcag cactcgacgc cttcacccgc gaggagctct ggggggtcct gcagcagctc
     7741 ttcatggatc gccgcttcac cgtgatcctg gttacccatg atctgcggga gtcggtctat
     7801 ctggccgata cggttcacgt cgtcagcaag cgccccgggc ggatcgtcaa gaccaaacat
     7861 atcgatctgc cccggccgcg aaccctcgag atgaccttcg agccgggctt cgtcgacatt
     7921 gtgcacgagc tgcgcgacgc catcagcgag atccgcggcg catgaccgac gcgaccaagc
     7981 agcggctggc tccctggatc gcgacggcag cgctcttcct cgtctgggag ctggcctgcc
     8041 gggtcttcgg cgtgccggag ttcatcctgc cgacgccgtc cgcgagcttc gcggccctct
     8101 tcgagtattt cgacgcggtg atgttcaatg cgtcgttcac gctctgggtg acgctcgtcg
     8161 gcttcgccat cgccgtggtg ttcggcctgg cgctgggtgt gctggtcggc gcctccgcgc
     8221 tcacctatgc ggcgctctac ccgatcctga tcggcttcaa cgcgatcccg aaggtggcga
     8281 tcgtgccgat cctggtgatc tggttcggca tcggcgccgt gccggcgatc ctgacggcct
     8341 tcctgatcag cttcttcccc atcgtcgtga acgtcgccac cggcatcgcc acgctcgagc
     8401 cggagctcaa ggacgtgctc cgctcgctgg gcgcccggcc gctcgacatc ctgctcaagg
     8461 tcggcctgcc gcgctcgatg ccctatttct tcgccagcct gaaggtcgcg gtgacgctcg
     8521 ccttcgtcgg ctcgatcatc agcgagacga tcgccggcaa tcgcggcatc ggctatctga
     8581 tgatgcaggc gtcatccagc ttccgcgtgc ccctggtctt cgccgggctc atcgtcgtcg
     8641 cggtcatggg tgtcggcatg tatgcggtct tcgcctatgt cgagcggcgg atgaccggct
     8701 gggcgttccg cggtcaggcc tgagcggtgc tcgcacccag cagatgcttg gcacagcgcc
     8761 cgagcgctgc ctcgaacgtg gcatcgatgg cgcgcacgct ctcggcgatc atcaggttct
     8821 cgacgagccc gaaatcctcg atctggaggc cgtcggcatc gacctggccg cgagggcccg
     8881 gactcgccgg gccgacccgc tcggcatagg gccgaaggtc gagggaccag gcgaagacgg
     8941 ggagaccccg ggcgatggcg aagccgatct cccaggccgt gcccgggtcc atcccggcgc
     9001 cgcggaaggg gctgatatcg gcgatgacgg cggcggaccg ctcgatcagg gcgacgttgc
     9061 ccagatagat cgcacgcgcg aggctcacgc cggcaccggt cggccgctcg gcgtcggtcg
     9121 gaaaaaggcc caggaggccg gccgccgcac aggccgcctt caggcgctcg gcgacctccg
     9181 caacgtccgg gaaaaagact tccggacccg ccagatagac ggctttcatg atgtcggccc
     9241 tcagaaagtc gggttgcgcc gcagccaagc catgcggtcc atggttcgga cctcgatcac
     9301 ctgttcctcc gacccgcgct cgatggtcag cgccaccgcc acgcccggcc cgcccgccgt
     9361 ccagaccgaa cggtagagtc cctccagcgt cgtcacgggc tcgccgttca cggcggtgac
     9421 gcgatcgccc ggcctgaggc cggcgcgttc cgccggcccg ccgggcgaga cgctgacgag
     9481 gctgacgccc cgttcgtcct cccggctggt gatgccgagc cagggacggg cgccgttggc
     9541 atggccattc gccagcatgt ccgcgaggat cggcggcagc agggcagccg gcacgaacat
     9601 gttgccgggg ctggccacgc cggggcccag ggcgtcgccc acctgcaacg agccgatgcc
     9661 gacgagcgcg ccgtcccggt cgagaagggc cgcaccgccg aactcccggt ggggcgggga
     9721 ggtgaagagg gcatcctcca gaagatactc ccagtagccg gtgaagacgc ggcgatcggc
     9781 gagctcgacc tgggtgccgt cgagggcccg catccggctg atcacgagca gcggatcgcc
     9841 actcgcgaca tcggcggcga tcgacagggg ctcggcctcg atgggcaaga gggcgcggac
     9901 gagcccgagg ccggtgccgt ggtcccaggc caccacatcg gccggtatgc ggcgcgcccc
     9961 ggcgtcgaag agctcgacga cgctcgcctc catgacgagg tagccgatgg tgagcgccag
    10021 gccctcgtcg tcgatgagca cggccgagcc gcgccggatg cggccgagcg tcgccgcggt
    10081 ccgggccgcg gccggaacct ccgcccgcag gccgagcacg gcatccagcg gcccatccgc
    10141 cctggcgccc ggcacccaca ggaagagcgc gagaagggcg agcacgccgc ccgtgaggat
    10201 cgggcgcccg accgcagccc tcgatcgtac ggatcggcaa gaggcggaca tcgctgtctc
    10261 ccgctgggcg aagcctgtcc ttcctcagat aggcggcgct cggccgccgc ccagcctcgg
    10321 cccctcagcc ggcccgcaac tgcgccatct ggttcggcag ctcgacgttc acctcgaggg
    10381 tcgagaagcc ggtgcccgcc tggaagtcga cattgacctt ctcctggtcg acatcgacat
    10441 atttgcggat cacctcgagc agctcgcgct gcagcagcgg caggaagtcc ggcccgcccc
    10501 cgccggtgcg gtcgagggcg acgaggacgg tgagccgttc cttggccttg ttggcggaat
    10561 cgtcacgccg attgaggcga aagaaatcga agaatctcac gcgctcctcc tcaacagcca
    10621 gtcgacgagg ccgcgcttct gcggggtgac gtaacgcagc tcgacatcct cgccgaggag
    10681 acgggcgacg gcatccttgt aggcttcgcc ggccttcgac ttgtcgtcga ggaccaccgg
    10741 catgccgagg ttggatgcct gcagcacgga cccgcattgc gggatcacgc cgagcaccgg
    10801 gatcgccagg atctccagca cgtcgtcgag cttcagcatc tcgccgcgct gcacccggtc
    10861 gaggtcgtag cgggtgagca gcagatgctg cttgaccggc tcgtcgccgt gctcggcccg
    10921 cttcgacttg ctgttgagga ttcccaggat gcgatcgctg tcgcgcaccg aggaaacctc
    10981 ggggttggtc acgacgatcg cctcgtctgc atggtagagc gccatgtagg cgccccgctc
    11041 gatgccggcc gggctgtcgc agatgatgta gtcgaagtcc ttcctgagct cctcgatcac
    11101 cttggccacg ccctcggcgg cgagggcctc cttgtcgcgc gtctgcgagg tcggcaggat
    11161 gaagaggttg tcgacccgct tgtccttgat cagcgcctgg ttgaggcgga tcccgtcgtt
    11221 gatgacgttg atgaaatcga agacgacacg ccgctcgcac cccatgatga ggtcgaggtt
    11281 gcgcaggccc acgtcgaaat cgatgatgac ggtcttcttg ccgcgcaagg cgagaccggt
    11341 gccgatggcc gcggcgct
//
LOCUS       DAOTFI010000163        11352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6795_length_11352_cov_6.837567, whole genome shotgun sequence.
ACCESSION   DAOTFI010000163 DAOTFI010000000
VERSION     DAOTFI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6795_length_11352_cov_6.837567"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..353)
                     /locus_tag="P5558_15950"
     CDS             complement(60..353)
                     /locus_tag="P5558_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY25862.1"
                     /translation="MTNDVEKGRAGQLFDDFLREQGTCEDTSERAVTRVLAFQLAAAM
                     REQKITKAAMARRLATSRSQLDRLLDPTNDSVSLATLARAARIVGRTLSVELQ"
     gene            complement(473..1576)
                     /locus_tag="P5558_15955"
     CDS             complement(473..1576)
                     /locus_tag="P5558_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HRY25863.1"
                     /translation="MNQPAPQTPAAQTPALRIQASRHFTAWLAEEGIGLAFTTYQSGK
                     LFLLGLQPDGRLAVHERTFNRAMGLAVHEPAEGGRRLYLSTLFQLWRFDDILNAGQTH
                     QGHDRLYVPRLAWTTGDLDIHDIALSADGTPVFVNTLFSCLATPDAATSFRPLWQPPF
                     ISRLAAEDRCHLNGLAMKEGRPAFVTAVARSDTADGWRDHRTKGGIVIDIATGETIAA
                     NLAMPHSPRLHDAGDGEKLYLLDSGTGRFGTIDQATGHFTEIAFCPGYARGLALHGRF
                     AVIGLSRPRDNKTFQGLPLDQALAAKGAAPRCGLLVVDLKTGDTPHWLRLEGIVEELY
                     DTAILPHTRRPMALGLKTDEIRRTISIGEPVRL"
     gene            1750..2655
                     /locus_tag="P5558_15960"
     CDS             1750..2655
                     /locus_tag="P5558_15960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25864.1"
                     /translation="MVSTAARGRAPLLPRWLTADHPLPWLAPMFVMILAFGIYPLAYS
                     IWLSLHQRNRFTRVDEFSGLANWVQAFGDQRFIDSLLVTVQYTLTCLVFQVVLGIAIA
                     LLLDCERRGYGLLRALMTLPLVVPPAVTGLMFLLMQDGQFGVLSYYLKLIGLVSPEAP
                     ILANPRTALWGVMAADIWQWTPFMVLILLAGLRALPKEPYEAAAIDGAGFLQCLWHLT
                     LPMLSRVIAVAVLIRTIDLFRIYDYVYAMTAGGPGTATETLSFYAGRVFGVANFNYAA
                     TLSLIMLIVLNVACFTFLKLARVRF"
     gene            2655..3536
                     /locus_tag="P5558_15965"
     CDS             2655..3536
                     /locus_tag="P5558_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25865.1"
                     /translation="MQGSPASRLARDLAAWLVVLVFVFPLFWWVLASVKPYTAIFAQP
                     PVYVDFEATAENYAVTIGGQSRITLEGGVGGQTGGASSYYSLPSIRDSVIVALGSTAL
                     ALVLATLASFGLSRFAMRGKDHVVFFVLAQRMLPPIAVAIPLFFIFRDLGLRDSHLGL
                     ILAHTLINLPLAVLLLKSFFDDVPVELDESAMLDGATRFQVFRMVVLPLVKGGLAATA
                     VLCFIFSWTEFLISLLLTTSIRTIPVKITTFVTSTGTEWGLITALGSAALVPSFIFIL
                     LVQKHLVRGLTLGSLKS"
     gene            3592..5115
                     /locus_tag="P5558_15970"
     CDS             3592..5115
                     /locus_tag="P5558_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25866.1"
                     /translation="MSFRDHDRKTFIADTARAFGQRRIGRRTFFRRMAMAGVGFSGFA
                     STFLGSARPFGGLTNLGTPMAMAQTPDDQMAWLKDVGKQFAGTTVRYTSEATPPTVVA
                     SELKGEFEAATGITVEIEIVPLEQVLAKATLDAQGQLGTYDLYYLDQAWSALFQPDVY
                     NPREYYEEKPELAMPGYDWDDFSPELVKAITTANGSIMGVPFDIPIFILMYRKDLYDK
                     HGLAVPETLGQFMEVTKALHEAESGNGIYGTTGQLRSGHYSLNCDWTAWLWANGGSIF
                     DRNRMFSGNDEAGMKGLDFMLELVEYMPPDAKTWTWDGEWQSMAQGLAGHVISWGEFF
                     PGLDGGDSKVAGLMEAVKPPEEAALRSPADAGFNEIPHIGHQGGSSIALSRYSKNPDA
                     AWLFMQWVTSKDVVARSSTVGGGASPVRLSAFKDPRVLEAAKPGAGTTRHFPAVEWTI
                     NNRMGSEPSDLPSWAEIANNVIPVELGKLLVGQTGSPEEAMAAIQSQTDELAAPFRG"
     gene            complement(5160..7322)
                     /locus_tag="P5558_15975"
     CDS             complement(5160..7322)
                     /locus_tag="P5558_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRY25867.1"
                     /translation="MNLQPDPETPGLWVNPRWQAGMPGSFAVVIGVSAYPHLDGGAEP
                     ADDHGQEWIAETRSLGQLYVSASTARRFFDWLATDYRYAKAPIARCWLLLSPTVAERA
                     HEPAVGDHAALPTLDNCETALKAWRKAIRDLPKAAQHDSRAFFFFSGHGLQVHHGKHL
                     LLPSSYLGGDEPSWDEAIGTSNLLYGLESLEIPDRFYFLDACRNDLPKLRAKRPEGQR
                     ILSEDEAALAFSGTRAGALLFATNSSLQAWQPTDPKDGVSLFGQAVLEGLAGRPDIEL
                     MPQDDRLAVVLTKLHGFTRERVLELMKQHQSLALQSVMLDGPRGEETTVTELAPEAVA
                     AFRPQPVQPPPLIRPPGPNEGEFRGGGPGGFGPAGMAPPARSAATRGLDLRRMRAKVL
                     LHRTALGPELVHGAWARDFDRGHEIFGSERVTDLWQNDTRVTTLAGRQRLGPDDIELH
                     SVAYDENARVYRLDVTFDVPDPLGYWVEAGGGMEGEERHGVVLPTDPAGSMTYRIEAD
                     IERIDGPNLYSRRLRRLDAWIAPTTEGPVGEAARLWRTYATADAETAALEFEAGALGD
                     MVRAKLESPLAATIAALVLLRAGRLDLIEGWLRNLSECFPTLPDGAVLWTEQVLRQEK
                     DREKAAAKATQSLVRLVQRGLPVTAEALSYAASQTERLHRARRLIPETLITPFDTLRE
                     RLAEALVAFRPNGLFTSYAGFAKGGARILLPGPPVRQK"
     gene            complement(7319..8608)
                     /locus_tag="P5558_15980"
     CDS             complement(7319..8608)
                     /locus_tag="P5558_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25868.1"
                     /translation="MPIIYVHGVNVRSRDGFLALTDYLRRYLAPVIADDPENVLIDDV
                     FWGDKGVSFAWGGQSRPRSQLIGMGAGARELQPVEGALAATAFAAALDRVPEPQAPAA
                     PATGGLAAGGPGGAAPPSARELRLSQLSREDLSDFLTAAIIAADPEPSPTRTARILAA
                     DAIAMDPATGAALARAKTPANEVRMAMALLKERSPAEAGLAGMGGGGFWADLGDRVSE
                     ATRRALGLPTYAASVVAAELRKPLNDMVSVFLGDVMVYLQGRGAVPEPGPIQRLLLDK
                     LSTAQANAASRNDEPLVVLTHSMGGQLVWDAVSHFMPRTPALRDVRIDFWCATASQVG
                     FFEDAKLFIERDPAYKTGHPVPFPKAQLGHWWNVWDHNDFISFTAKGIVDGVDDEAYS
                     SGMSLISAHGGYLERPSFFRQFALKLEAARAKGWSRP"
     gene            complement(8671..10185)
                     /locus_tag="P5558_15985"
     CDS             complement(8671..10185)
                     /locus_tag="P5558_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRY25869.1"
                     /translation="MPGKGPREGSEAVTLGLDIGTTSTIGVLAATDGRILATATRPAT
                     LRSPRPGWAEADPLEWWRNVQALVPELFAAAGIGASAIAGIGVAGMLPAVVLLDGAGR
                     VLRPSIQQSDGRTGAEVEAMRRELDEAAFTARTGNGINQQLVGTKLRWLERHEPEVMA
                     RAATVMGAYDFINLRLTGERTVERNWALEAGFVDLATGEAAADLLAFAHLDPARLPRV
                     APGEAVIGAVTAEAAALTGLAAGTPVVAGAADHIASAFVAGIVRPGDVLLKFGGAGDI
                     MLAADAPRPDPRLFLDYHIVPGRFMPNGCMAASGSLLNWLVREFAGGRDHAALDTLAA
                     SVPPGADGLVLLPYVLGEKTPIHDPAARGTLVGLGLHHRIDHLWRAALEAVVMGFRHH
                     IEVAREIGHPVERLVASDGGSRSALWMQIAADALQLPVTTLDGHPGSCLGAAWVAAMG
                     TGAADDWSAIARFTRPAATFEPDPANAPACDHLYGLYRETYDRLKPLYPKFAAP"
     gene            complement(10191..10985)
                     /locus_tag="P5558_15990"
     CDS             complement(10191..10985)
                     /locus_tag="P5558_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HRY25870.1"
                     /translation="MLPPFGTKKPIIAMAHLGPLPGAPGHDRDGGIRKLTDDVAADIE
                     NLQAAGVDAIMFGNEGDRPYVLQAPPEGLATMAAIVASLKPSIRVPLGVNYLWDPVAT
                     VALAVATGALFAREIFTGVYASDMGLWQPKCAEALRLRDNLGRGDLKLLFNINAEFAA
                     SLDTRPAALKAKSAVFSSKADIICVSGPITGQAADQSELRAVKEALPETPVFANTGVT
                     IDNVADTLSIADGVVIGSHFKRDGNTWNPVDRDRAARFMDRVRQLR"
BASE COUNT         1604 a         3505 c         4403 g         1840 t
ORIGIN      
        1 acaacgtcga tatcagcttt catcgacatg ttgcgcttcg cacatgaatg caccccctgc
       61 tactgcagct cgaccgacaa ggtgcgcccg acgattctgg cggcgcgggc cagggtggcg
      121 aggctcacgc tgtcgttggt ggggtcgagg aggcggtcga gctgggagcg gctggtggcg
      181 aggcgtctgg ccattgcggc cttggtgatc ttctgctctc gcatggccgc ggcgagctgg
      241 aaggcgagga cgcgcgtgac ggcgcgttcg ctcgtgtcct cgcaggttcc ctgctcgcgc
      301 aggaagtcgt cgaagagctg tccagccctg cccttctcga catcgttcgt catgacagcc
      361 ctcgcgttct ttgctcctcc gagcatacgc gaccaagcga cccgaagtaa tagttcgctc
      421 caaaaacccg atgggcgcgg gaaggccagc cttcccgcct cttacccttt cctcagagcc
      481 gcaccggctc gccgatgctg atggttcggc ggatttcgtc ggtcttgagg ccgagggcca
      541 tggggcgacg ggtgtggggg aggatggcgg tgtcgtagag ttcctcgacg atgccttcga
      601 ggcggagcca gtggggggtg tcgccggttt tgaggtcgac gacgaggagg ccgcagcggg
      661 gggcagcacc tttggcggcg agggcttggt cgagggggag gccctggaag gtcttgttgt
      721 cgcgggggcg ggagaggccg atgacggcga agcggccgtg gagggcgagg ccgcgggcgt
      781 agccggggca gaaggcgatt tcggtgaagt ggccggtggc ctggtcgatg gtgccgaagc
      841 ggccggtgcc ggaatcgagg aggtagagct tttcgccatc tccggcgtcg tggaggcggg
      901 gggaatgggg catggcgagg ttggcggcta tggtttcgcc ggtggcgatg tcgatgacga
      961 tgccgccctt ggtgcggtgg tcgcgccagc cgtcggcggt gtcggagcgg gcgacggcgg
     1021 tgacgaaggc ggggcggcct tctttcatgg cgaggccgtt gaggtggcag cggtcctcgg
     1081 cggcgagcct cgagatgaag gggggttgcc agagggggcg gaagctcgtg gcggcgtcgg
     1141 gggtggcgag gcaggagaag agggtgttga cgaagacggg ggttccgtcc gcgctcaagg
     1201 caatgtcgtg gatgtcgagg tcgccggtgg tccaggcgag cctcgggacg tagaggcggt
     1261 cgtggccctg gtgggtctgg ccggcattga ggatgtcgtc gaaccgccag agctggaaga
     1321 gggtggagag gtagagccgg cggccccctt cggcaggttc gtggacggct aggcccatgg
     1381 cgcggttgaa ggttcgctcg tggacggcga ggcggccgtc cggctggagg cccaggagga
     1441 agagcttgcc ggactggtag gtggtgaagg cgaggccgat gccttcctcg gcgagccagg
     1501 cggtgaagtg gcgggacgcc tggatccgga gggcgggggt ctgggcagca ggcgtctggg
     1561 gggctggctg gttcatggag cagtgattag ggccgggcgg gcgaatcgtc cattcggggc
     1621 gggggcggcg ggcttcactt gacttgccgg agcgtggcgg caaactggcg tgaacatcgt
     1681 tcgggccagt gaacaaatgt acaggaagcg tgcaattcgg gtcggccctg gctgcgacat
     1741 gacggggccg tggtgagcac ggccgcccgg ggccgcgcgc cccttttgcc gcgctggctg
     1801 acggcggatc atccgctgcc ctggctggcg ccgatgttcg tcatgatcct ggcgttcggc
     1861 atctaccccc tggcctactc gatctggctc agcctgcacc agcgcaaccg cttcacccgg
     1921 gtggacgagt tcagcgggct cgccaactgg gtgcaggcgt tcggcgacca gaggttcatc
     1981 gattcgctcc tggtgaccgt gcagtacacg ctgacctgcc tcgtcttcca ggtggtgctg
     2041 gggatcgcca tcgcgttgct cctggattgc gagcggcggg gctacgggct gctcagggcc
     2101 ctcatgaccc tgcccttggt ggtgccgccg gccgtcaccg ggctcatgtt cctcctgatg
     2161 caggacgggc agttcggggt gctctcgtat tatctcaagc tcatcgggct ggtgagcccc
     2221 gaggcgccga tcctggcgaa cccgaggacg gcgctctggg gggtgatggc ggcggacatc
     2281 tggcagtgga cgccgttcat ggtcctgatc ctgctggcgg gcctgcgcgc cctgccgaag
     2341 gagccctacg aggcggcggc gatcgacggg gcggggtttt tgcagtgcct gtggcatctg
     2401 accctgccga tgctgagccg ggtgatcgcg gtggcggtgc tgatccggac gatcgatctc
     2461 tttcgcatct acgactatgt ctatgcgatg acggcgggcg ggcccgggac ggcgacggag
     2521 acgttgagct tctatgcggg gcgggtcttc ggtgtggcca acttcaatta cgcggcgacc
     2581 ttgagcctga tcatgctgat cgtcctgaac gtcgcctgct tcaccttcct caagctggcg
     2641 cgggtgcggt tctgatgcag ggaagtcccg cgagtcggct cgcccgcgac ctcgccgcct
     2701 ggctggtggt gctggtcttc gtctttccgc tcttctggtg ggtgctggcc tcggtcaagc
     2761 cctacacggc gatcttcgcg cagccgccgg tctatgtgga tttcgaggcc acggccgaga
     2821 actatgcggt gaccattggc gggcagtcca ggatcacgct ggagggcggg gtcggcgggc
     2881 agacgggcgg ggcgtcgtcc tattattcgc tgccttcgat cagggacagt gtgatcgtgg
     2941 cgctgggctc gacggcactg gccctggtgc tggcgacgct tgcctcgttc gggctctcgc
     3001 gcttcgccat gcggggcaag gaccatgtgg ttttcttcgt gctggcgcag cggatgctgc
     3061 cgccgatcgc cgtcgccatc ccgctcttct tcatctttcg ggacctcggg ctccgcgaca
     3121 gccatctcgg gctgatcctg gcgcatacgc tgatcaacct gccgctggcg gtgctgctct
     3181 tgaagagctt cttcgacgac gtgccggtgg agctcgacga atcggcgatg ctggacggcg
     3241 ccacgcggtt tcaggtcttc cgcatggtcg tgctgccgct ggtcaagggc gggctggcgg
     3301 cgacggcggt gctctgcttc atcttctcct ggacggagtt cctcatctcg ctcctgctca
     3361 ccacgagcat ccggacgatt ccggtgaaga tcaccacctt cgtcacgtcg accggcacgg
     3421 aatgggggct gatcacggcg ctcggcagcg ctgccctggt gccgagcttc atcttcatcc
     3481 tgctggtcca gaagcatctc gtgcgcggcc tcaccctcgg ctccctgaag agctgagcgg
     3541 ggccgggcgc gggggtaggc gaaacgcggc tcaaccttag ggaggtcttc catgagcttt
     3601 cgcgaccacg acagaaagac gttcatcgcc gatacggcgc gggcgttcgg ccagcggcgg
     3661 atcggccggc gcaccttctt ccggcggatg gccatggccg gcgtcggctt ctcgggcttc
     3721 gcctcgacgt tcctgggctc tgcccggccg ttcggcgggc tcaccaatct cggcacgccc
     3781 atggcgatgg cgcagacgcc ggacgaccag atggcctggc tcaaggacgt gggcaagcag
     3841 ttcgccggca cgacggtgcg ctacacctcg gaggcgacac cgccgacggt ggtggcctcc
     3901 gagctgaagg gcgagttcga ggcggcgacg gggatcacgg tcgagatcga gatcgtgccc
     3961 ttggagcagg tgctggccaa ggcgacgctg gacgcccagg gccagctcgg cacgtatgac
     4021 ctctattatc tggaccaggc ctggtcggcg ctcttccagc ccgatgtcta caacccgcgc
     4081 gagtactacg aggagaagcc cgagctggcg atgccgggct atgactggga cgatttctcg
     4141 ccggagctgg tcaaggcgat caccaccgcc aacggctcga tcatgggcgt gccgttcgac
     4201 atcccgatct tcatcctgat gtacaggaag gacctctacg acaagcacgg cctggcggtg
     4261 ccggagacgc tcgggcagtt catggaggtc acgaaggcgc tgcacgaggc cgagagcggc
     4321 aacggcatct acggcaccac gggccagttg cgctccggcc actacagcct gaattgcgac
     4381 tggacggcct ggctctgggc caatggcggg tcgatcttcg acaggaaccg gatgttctcc
     4441 ggcaacgacg aggccgggat gaaggggctc gacttcatgc tcgagctggt cgagtacatg
     4501 ccgcccgatg ccaagacctg gacctgggac ggcgagtggc agtcgatggc gcaggggctc
     4561 gccgggcacg tcatctcctg gggcgagttc ttccccgggc tcgacggcgg cgacagcaag
     4621 gtggccgggc tgatggaggc ggtcaagccg cccgaggagg cagcgctgcg ctcgcccgcg
     4681 gatgccggat tcaacgagat cccgcatatc ggccaccagg gtgggtcgtc gatcgccctc
     4741 tcgcgctact cgaagaaccc ggacgcggcc tggctcttca tgcagtgggt gacctcgaag
     4801 gacgtggtgg cgcgctcgtc cacggtcggc ggcggcgcct cgccggtccg gctctcggcg
     4861 ttcaaggacc cgcgcgtgct ggaggcggcg aagccgggtg ccggcaccac gcggcatttc
     4921 ccggcggtcg agtggacgat caacaaccgc atgggctccg agccgtcgga cctgccgtcc
     4981 tgggccgaga tcgccaacaa cgtcatcccg gtcgagctcg gcaagctcct ggtcggccag
     5041 accggctcgc cggaggaggc gatggcggcg atccagagcc agacggacga gctggcggcg
     5101 ccgttcaggg gctgaacggt cttttccggt cgatgggggg tcggctcgcg ccggccccct
     5161 tacttttgcc ggacaggcgg gccagggagc aggatgcggg ccccgccctt ggcgaagccg
     5221 gcgtagctgg tgaaaaggcc gttcgggcgg aaggcgacga gggcttcggc gaggcgctcg
     5281 cggagcgtgt cgaagggggt gatcagggtt tcggggatca ggcggcgggc acggtgcagg
     5341 cgctccgtct ggctcgcggc gtagctcagc gcctcggcgg tgacgggcag gccgcgctgg
     5401 acgaggcgga cgagcgattg cgtggccttg gcggcggctt tttcgcggtc cttttcctgg
     5461 cgcaggacct gctcggtcca caggacggcg ccatcgggca gcgtggggaa gcactcggag
     5521 aggttccgca gccagccctc gatgaggtcg agacggccgg cgcgcaggag cacgagtgcg
     5581 gcgatggtgg cggcgagcgg cgattcgagc ttcgcccgga ccatgtcgcc gagggcgccg
     5641 gcctcgaact cgagggccgc cgtttcggca tcggcggtgg cgtaggtgcg ccagaggcgg
     5701 gccgcctcgc cgaccggtcc ctcggtcgtg ggggcgatcc aggcgtcgag gcgcctgagg
     5761 cggcggctgt agaggttcgg gccgtcgatc cgctcgatgt cggcctcgat ccggtaggtc
     5821 atagagccgg ccgggtcggt cggcaggacg acgccgtgcc gctcctcgcc ctccatgccg
     5881 ccgccggcct cgacccagta gccgagcggg tcggggacgt cgaaggtgac gtccagccgg
     5941 tagacgcggg cgttctcgtc ataggcgacg ctgtgcagct cgatgtcgtc cgggccgagc
     6001 cgctggcggc cggcgagcgt ggtcacgcgg gtgtcgttct gccagaggtc ggtgaccctt
     6061 tcgctgccga agatctcgtg gccgcgatcg aagtcgcgcg cccaggcgcc gtgcaccagc
     6121 tcgggcccga gcgccgtgcg gtgcagcagc actttggcgc gcatgcggcg aagatcgagg
     6181 ccgcgggtgg cggccgagcg ggccggcggg gccatgccgg cggggccgaa gccgcctggg
     6241 ccgccacccc ggaattcgcc ttcatttggc ccgggtggcc tgatgagcgg tggcggctgg
     6301 accggctggg ggcggaacgc cgccacggct tcgggggcga gctcggtgac ggtggtctcc
     6361 tcgccgcgcg ggccgtcgag catcaccgat tgcagcgcca aagactggtg ctgcttcatc
     6421 agctccagca cgcgctcgcg ggtgaagccg tgcagcttgg tcaggaccac ggcgagcctg
     6481 tcgtcctgcg gcatgagctc gatgtcgggc cggccggcga ggccctcgag gacggcctgg
     6541 ccgaagagcg agacgccgtc tttggggtcg gtcggctgcc aggcctggag cgagctgttg
     6601 gtggcgaaga gcagggcccc ggcgcgcgtc ccggagaacg ccagggccgc ctcgtcctcg
     6661 gagaggatgc gctggccctc ggggcgcttg gcccggagct tcggcaggtc gttgcggcag
     6721 gcgtcgagga agtagaagcg gtcggggatc tccagcgatt ccaggccgta gagcaggttg
     6781 ctggtgccga tggcctcgtc ccagctcggc tcgtcgccgc cgagatagct cgacggcagg
     6841 aggagatgct tgccgtgatg gacctgcagc ccgtggccgc tgaagaagaa gaaggcgcgg
     6901 ctgtcgtgct gggcggcctt gggcaggtcg cgaatggcct tgcgccaggc cttgagcgcg
     6961 gtttcacagt tgtcgagcgt gggcagggcg gcatggtcgc cgacggccgg ctcgtgcgcc
     7021 cgctcggcga cggtcggcga gagcaggagc cagcatctgg cgatgggtgc cttggcgtag
     7081 cggtagtcgg tggcgagcca gtcgaagaag cgcctcgcgg tcgaggccga gacgtagagc
     7141 tgcccgagcg agcgggtttc ggcgatccat tcctggccgt ggtcgtcggc cggctcggcg
     7201 ccgccgtcga gatgggggta ggcgctgacg ccgatgacca cggcgaagct gcccggcatg
     7261 cccgcctgcc agcggggatt gacccagagc ccgggtgttt ccgggtccgg ctggaggttc
     7321 aagggcggct ccagcccttg gctctggcgg cttccagctt cagggcgaac tggcgaaaga
     7381 agctggggcg ctcgagatag ccgccatggg cggagatcag cgacatgccg ctggaatagg
     7441 cctcgtcgtc gacgccgtcg acgatgccct tggcggtgaa gctgatgaag tcgttgtggt
     7501 cccagacgtt ccaccagtgg ccgagctggg ccttggggaa ggggacggga tggccggtct
     7561 tgtaggccgg gtcccgctcg atgaagagct tggcgtcctc gaagaagccg acctggctcg
     7621 ccgtggcgca ccagaaatcg atgcgcacgt cgcgcagcgc cggggtgcgc ggcatgaagt
     7681 gggagaccgc gtcccagacg agctggccgc ccatgctgtg ggtgaggacg accaggggct
     7741 cgtcgttccg gctcgcggcg ttggcctggg cggtgctcag cttgtccagg aggaggcgct
     7801 ggatcgggcc cggctccggc acggcgcccc ggccctgcag gtagaccatg acgtcgccga
     7861 ggaagacgct gaccatgtcg ttgagcggct tcctgagctc ggccgcgacc accgaggcgg
     7921 cataggtcgg caggcccagg gcccggcgcg tggcttccga gacccggtcg ccgagatcgg
     7981 cccagaagcc gccgccgccc atgccggcga ggccggcctc ggccgggctc cgctccttga
     8041 gcagcgccat ggccatgcgg acctcgttgg ccggggtctt ggcccgggcg agggcggcgc
     8101 cggtggcggg atccatggcg atggcgtcgg cggcgaggat gcgggcggtg cgcgtcggcg
     8161 agggctcggg atcggcggcg atgatggcgg cggtgaggaa gtccgagagg tcctcgcgcg
     8221 agagctggct gaggcgcagc tccctggcag acggcggtgc ggctccgccc ggcccgccgg
     8281 cggcgaggcc gcccgtggcg ggtgcggcgg gtgcttgcgg ctccggcacg cgatcgaggg
     8341 cggcagcgaa ggcggtggcg gcgagggcac cctcgaccgg ctgcagctcg cgggcgccgg
     8401 cacccatgcc gatgagctgc gagcgcggcc gcgactggcc gccccaggcg aagctgacgc
     8461 ccttgtcgcc ccagaagacg tcgtcgatca gcacgttctc ggggtcgtcg gcgatgaccg
     8521 gggcgaggta gcggcggaga tagtcggtga gggcgagaaa gccgtcgcgc gagcgcacat
     8581 tgacgccgtg gacgtagatg atcggcatga accgccctcc cctgatccga tcgcagagga
     8641 gggggagtat ctgccgagtt caggcggcga tcaaggtgcg gcgaacttcg ggtagagcgg
     8701 cttcaggcga tcgtaggttt ctcggtagag gccgtagagg tggtcacagg ccggggcatt
     8761 ggccgggtcc ggctcgaagg tggcggccgg gcgggtgaag cgggcgatgg cggaccagtc
     8821 gtcggcggcc ccggtgccca tggcggcgac ccaggcggca ccgaggcagg agccgggatg
     8881 gccgtcgagc gtggtcaccg ggagctgcag ggcgtcggcg gcgatctgca tccagagagc
     8941 gctcctcgag ccgccgtcgc tggcgacgag gcgctcgacc gggtggccga tctccctggc
     9001 cacctcgatg tggtggcgaa agcccatgac gacggcctcc agggcggcgc gccagaggtg
     9061 gtcgatgcgg tggtggaggc cgagcccgac cagcgtgccg cgggcggcgg gatcgtggat
     9121 cggggtcttc tcgccgagca cgtaggggag gaggacgagc ccgtcggcgc cgggcgggac
     9181 ggaagccgcc agcgtgtcga gggcggcatg gtcgcggccg ccggcgaact cgcgcacgag
     9241 ccagttgagc agcgagcccg aggccgccat gcagccgttc ggcatgaagc ggccggggac
     9301 gatgtggtag tcgaggaaga ggcggggatc gggccttggg gcatcggcgg cgagcatgat
     9361 gtcgccggcc ccgccgaact tgagcagcac gtcgcctggc ctgacgatgc cggcgacgaa
     9421 ggccgaggcg atgtggtcgg cggccccggc gacgaccggc gtgccggcgg cgaggccggt
     9481 gagcgccgcg gcttcggcgg tcacggcgcc gatcaccgcc tcgcccgggg cgacgcgcgg
     9541 caggcgggcc gggtcgagat gggcgaaggc gaggaggtcg gcggccgcct cgcccgtggc
     9601 gagatcgacg aagccggctt cgagcgccca gttgcgctcg accgtgcgct cgccggtcag
     9661 gcggaggttg atgaagtcgt aggcgcccat gacggtggcg gcccgggcca tgacctcggg
     9721 ctcgtggcgc tccagccagc ggagcttcgt gccgacgagc tgctggttga tgccgttgcc
     9781 ggtgcgcgcg gtgaaggcgg cctcgtccag ctcgcggcgc atggcttcca cctccgcgcc
     9841 ggtgcggccg tcgctctgct ggatcgacgg gcgcagcacc ctgcccgcgc cgtcgagcag
     9901 gacgacggcc ggcagcatgc cggcgacgcc gatgccggcg atggccgagg ccccgatgcc
     9961 ggccgcggcg aagagctccg gcaccagcgc ctggacgttt cgccaccatt ccaggggatc
    10021 ggcctcggcc cagcccggcc gcggcgagcg gagcgtcgcc gggcgggtgg cggtggcgag
    10081 gatccggcca tcggtcgcag cgagcacgcc gatggtcgag gtggtgccga tatcgaggcc
    10141 gagggtgacg gcctcactgc cctctcttgg gccctttcct ggcatggcgg tcagcggagc
    10201 tggcgcacgc ggtccatgaa gcgggcggcg cggtcgcggt cgaccgggtt ccaggtattg
    10261 ccgtcacgct tgaagtggct gccgatgacc acgccgtcgg cgatcgagag ggtgtccgcg
    10321 acattgtcga tggtgacgcc ggtattggcg aagaccgggg tctccggcag cgcttccttc
    10381 accgcccgca gctccgactg gtccgctgcc tggccggtga tcggcccgga gacgcagatg
    10441 atgtcggcct tcgacgagaa gacggcgctc ttcgccttga gggctgccgg gcgagtgtcg
    10501 agcgaggccg cgaactcggc gttgatgttg aagaggagct tgaggtcgcc gcgcccgaga
    10561 ttgtcgcgca ggcggagtgc ttcggcgcat ttcggctgcc agaggcccat gtcggaggcg
    10621 tagacgccgg tgaagatctc gcgggcgaag agggcgccgg tggcgacggc gagggccacc
    10681 gtggcgaccg ggtcccagag atagttcacg cccaaaggca cccggatgga gggcttcagg
    10741 gaggccacga tggcggccat ggtggcgagg ccctcgggcg gggcctggag cacgtagggg
    10801 cggtcgccct cgttgccgaa catgatggcg tcgacgccgg ccgcctgcag gttctcgatg
    10861 tcggcggcga cgtcgtccgt gagcttccgg atgccgccgt cgcggtcatg gccgggagca
    10921 cccggcagcg gtcccagatg ggccatggcg ataatgggct tcttcgtccc gaacggcggc
    10981 agcatgtcgt ctcctctggc atgttggccg accgatcctg ccgggaaatc gcgcgtgacg
    11041 gtagccctgc cgcaccgatc ctggcgggca acttgccttt cgggagagcc gccacccgct
    11101 tcttcccgta accgaccata gacggcgtgc accccacgcg agagtatacg ttcgccggtt
    11161 gcaacctgta cccgatgctt gcgctcgggc gcggaaacaa tgatttggga gagacgtcat
    11221 ggccgctgtc gacgatcgtg caacgaccaa cgaggacacg ccggtcacga ccaacgtcat
    11281 tgccaacgac cgtgatcctg cgggcgggat cgcacggctc gaggccatcg tcacgccccc
    11341 gcgcgatcgc gg
//
LOCUS       DAOTFI010000164        11312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6830_length_11312_cov_5.967309, whole genome shotgun sequence.
ACCESSION   DAOTFI010000164 DAOTFI010000000
VERSION     DAOTFI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6830_length_11312_cov_5.967309"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /gene="uvrA"
                     /locus_tag="P5558_15995"
     CDS             complement(<1..2099)
                     /gene="uvrA"
                     /locus_tag="P5558_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRY25871.1"
                     /translation="MNDVIRIRGAREHNLKSVDVDIPRFRLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLELMQKPDVDLIEGLSPAISIEQKTTSKNPRSTVATV
                     TEIYDYMRLLWARVGVPYSPATGLPIEAQTVSQMVDRVLAMPEGSRLYLLAPIVRGRK
                     GEYRKELRELQRQGFQRVKIDGEFHEIDEAPALDRKLKHEIEVVVDRLVTAPDLAQRL
                     ADSIETALGLSDGLLVVEDADSGERTTHSAKFACPVSGFTLPEIEPRLFSFNNPYGAC
                     PSCDGLGRRLLVDPELVVPDATKTVHQGAVEPWSSQTMHYYPQALAAVLRHFDESLHT
                     PWEDLKEETRQTILYGSGKEPVPMSFEDGLRTMKTNKPFEGVILNLQRRWRETDSAWM
                     REEIQRYMSSAPCAACEGHRLKPEALSVRVGGRHIAAVSELSIQEAALWFDDLAGRLD
                     AKQATIAERILKEIRERLGFLVNVGLSYLTLNRDSGTLSGGESQRIRLASQIGSGLTG
                     VLYVLDEPSIGLHQRDNDRLLATLTRLRDLGNTVIVVEHDEDAIRTADHLIDMGPGAG
                     VHGGIVVAQGTPEEVIASSASLTGQYLAGHREIPLPARRRRPKAGRWLELGGATANNL
                     REVDAHFPLGCFVCVTGVSGSGKSSLVVDTLYPALAKRLTGARQMPGPHRELTGLELV
                     DKVVDIDQSPIGRTPRS"
     gene            2267..2770
                     /gene="ssb"
                     /locus_tag="P5558_16000"
     CDS             2267..2770
                     /gene="ssb"
                     /locus_tag="P5558_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRY25872.1"
                     /translation="MAGSVNKVILVGNLGRDPEVRYTQSNQKIVHLAVATSERWKDRQ
                     TGEQREKTEWHRVVIFNENLADVAERYLQKGRSVYLEGQLQTRKWTDQSGQEKYTTEI
                     VLQRFRGELVLLGGGRDDMGSGDRDMVGGSYDDPGDRAAPAARGGRPAGGGRPAAADD
                     LDDDIPF"
     gene            complement(2778..3278)
                     /locus_tag="P5558_16005"
     CDS             complement(2778..3278)
                     /locus_tag="P5558_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25873.1"
                     /translation="MAASSCLLDKLRALIVHDGTPATERQAALDRLERLLVGHDGTVR
                     QRTELTRSARIGRQRRRLPIDEWARLEPYAGGLSLGECIGRLAAACSGHGVLPQRIDF
                     GRHGTGDRPVIAVQFPLPEAPNPIAFAASVRNHFPGASVNVANQQAEGERVFLVYLPG
                     RVADSS"
     gene            complement(3355..4053)
                     /locus_tag="P5558_16010"
     CDS             complement(3355..4053)
                     /locus_tag="P5558_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRY25874.1"
                     /translation="MTIDQELLDHLAAFDTPTICNALEVVQGKRLTVGFTSSQMVCAF
                     PDMKPIVGFARTATIKAATPSPLPPAEAKALRIGYYEYLVKGPQPVITVIQDLDPEPG
                     FGAFWGEVNTAVHRGLGVLGCVTNGSIRDLDAIDPRFQLLAGKIGPSHADVHVEAIEV
                     PVEIYGMKVVHGDLVHADRHGAVVIPAGDAAALPAAIDLCIRRERPVLEAARSGNFDI
                     DALKAAFATAADIH"
     gene            4239..7574
                     /locus_tag="P5558_16015"
     CDS             4239..7574
                     /locus_tag="P5558_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRY25875.1"
                     /translation="MTIQVALNHQTRYGYDRRINLGPHVVRLRPASHSRTRILSYSLK
                     VSPTEHFINWQQDPHGNWLARLLFNEPTDHLSVEVDLVAEMAVLNPFDFFVEAAADTW
                     PFAYSASLFKELLPFLETETPGPLMAELVASIPREPVTTIDFIVGLNQRLQEMIGYLI
                     RMEPGIQSCEETLAQRSGSCRDTAWLFVQLLRQLGLAARFVSGYLIQLKADQAALDGP
                     SGTDVDFTDLHAWTEVFLPGAGWVGLDPTSGLLAGEGHIPLAATPDPGNAAPITGTLD
                     PAEVSFDFSMTIERIQEGPRVTLPYTEAEWASIDALGHAIDARLQAQDVRLTVGGEPT
                     FVGLDDRDGDEWNTAAVGPTKRQFADTLLRRLADRFAPGGLLQHGQGKWYPGEPLPRW
                     AFAVYWRTDGEPLWADHSLIASEEAAAERPHAALVEVAERFTAQLAANLELDPAHVHP
                     AYEDTAQFVLREQQLPANVDPLDSKLEDAEERARLARVFGRGLTTPTSYVLPLQPWQA
                     RASAGAPSPGRRRFRWMSERWGTRRGGLFLLPGDSPAGFRLPLGALDHLRPDAYPSVV
                     PLDPFAAHAALPAREPVQQPRHAQGYVPVQPGDRPVPGEPVRTALTVEPREGHLYVFL
                     PPVPSAEEYVDLIAAVEETALALGQPIRLEGYPPPADPRLRNIKVTPDPGVIEVNVQP
                     AASWAEQVRITEAIYEEARQTRLDTAKFMLDGRPTGTGGGNHIVVGGPTAADSPFLRR
                     PDLLASLIRYWQNHPSLSYLFSGLFIGPTSQAPRLDEARQDQLYELEIALARVPDPTR
                     GSDLDCPPWLVDRLFRHLLVDLTGNTHRAEICIDKLYSPDGPTGRLGLVEFRAFEMPP
                     HPRMSLAQNLLLRALIAWFWQRPYTGRLIRHGTDLHDRFMLPHYVWADFRSVLADLEA
                     AGMGFDPAWFAAHQEFRFPLYGHVTVADVEVELRAALEPWHVLGEEATGGGTARYVDS
                     SLERVQVRVTGDRQGRYVVTCNGYTLPLEPTGDGHEVAGVRFRAWQPPSCLHPTIPVH
                     SPLVFDVVDGVQGRSLGGCTYHVMHPGGRSYDTAPVNDFEAQGRRLARFQPMGHTPGS
                     MRPIAVPRNPSFPHTLDLRRVGI"
     gene            7590..10142
                     /locus_tag="P5558_16020"
     CDS             7590..10142
                     /locus_tag="P5558_16020"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRY25876.1"
                     /translation="MALTLSVRTETIDGLVAGYRPPGNQRDELLGPGGEVRRTWQPFL
                     ERFGAMGAEGRDAVARTTAQKLQESGIAFNVHADPDDRKAAWRLDLLPVILAADEWQG
                     LERGLVQRARLIEAVLADLYGAQRLLRTGVLPPSLVFGNPEFLHGCAGWSRPPRRFLF
                     HFACDVARDPNGGWRVLADQVDAPAGNGWLLASRVALSQGMGELYLESGVRRVAGYYA
                     RMQAALQAAAPVDEGRIVTLSPGARDPGFFSHAYLARYLGLALAEAADLTERDGHVYL
                     KTLEGLQRVDVVLRKIGGRFADPLNLSTRHDGGTPGLVQAARLGRTFFANALGSGVLQ
                     NRVLAAFSTSLAETLLGEALLLRESPALWLGDALAQERVLEEPGWVTSRLAARHDPGS
                     SDRHDPLPALADDEARRRFLARDGHGWVAERSSPLPTTPGFQEGRLTPVHWAMRAFVC
                     VDEGGYHVLPGGLVRQSPTPGTIGLPNGFGSKDLWVLREPGEVLAPSLFTRRFAQVHL
                     RRTGRDLLSRTAEALFWLGRNVERAESVMRTLRAVLTRLIDDGGPDRQPDLLEALLAI
                     HLGEERPLEGSARRRIGAGLERLLHDPEPMFGVRRSMAGIRHNATTARAVLSQDGWRV
                     LNALHADRRWRSAGRGILAAPPIDMVNDGIRQLVAFAGTAAENMTRNYSWRFLDMGRR
                     LERALQLIDLIEALTGTLDGIDEGVALTALLEIGDSFMTYRSRYAVTPMATPVVDLLA
                     LDETNPRSLAYQLLTLEGHLASLPSEGPYRSAEQRRVLSLLTELQLADADQLTADREQ
                     GAASVLAGFFARARVDLREAADLIGRGYFVLAETPTSTLAMRRVEGLGGVGP"
     gene            10139..11020
                     /locus_tag="P5558_16025"
     CDS             10139..11020
                     /locus_tag="P5558_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRY25877.1"
                     /translation="MIFDVRHRTDYSYSQLVSISHHLLHLEPRDTPHQRIERFELSIE
                     PSPGFDDRDVDTFGNPTRTITLDQNHDELVIEARSRVRLAEREVPPPLDTPAWEEAAA
                     VRLAATDDDALEASRYAFASPFVDLGDAVEAYARECFLPGRPILDAALELNGRIFREF
                     TFDNKATSLATPVAEVLETRRGVCQDFSHLMLACLRSLGLPARYQSGYIMTRPPEGQE
                     RLVGADASHAWVALWVPRHGWVELDPTNDCLPMREHVTLAWGRDYGDVSPISGIVYGG
                     GAHDLTVAVDVVPLVPA"
     gene            11031..>11312
                     /locus_tag="P5558_16030"
     CDS             11031..>11312
                     /locus_tag="P5558_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY25878.1"
                     /translation="MEVDFIVVGAGSAGCALASRLAEDGRSTVLVLEHGGTDAGPLIQ
                     MPAALSIPMNMRRYDWGYRTEPEPGLDGRRLACPRGKVIGGSSSINGMVY"
BASE COUNT         1615 a         4015 c         3978 g         1704 t
ORIGIN      
        1 ttggagcggg gcgtgcggcc gatcggcgac tggtcgatgt cgaccacctt gtcgacgagc
       61 tcgaggccgg tgagctcgcg gtgcgggccc ggcatctgcc gcgccccggt caagcgcttc
      121 gcaagggcgg ggtagagcgt gtcgaccacc aggctcgact tgcccgagcc cgagacgccc
      181 gtgacgcaga cgaagcagcc gagggggaag tgggcgtcga cctcgcgcag gttgttcgcc
      241 gtggccccgc cgagctccag ccagcggccg gccttcggcc ggcgccggcg ggcgggcagg
      301 gggatctcgc ggtggccggc gaggtactgg ccggtcaggc tggcggacga cgcgatcacc
      361 tcctcgggcg tgccctgggc caccacgatg ccgccatgca cgccggcccc cggccccatg
      421 tcgatgaggt ggtcggcggt gcggatcgcg tcctcgtcgt gctcgaccac gatcaccgtg
      481 ttgccgagat cgcgcagccg ggtgagcgtc gcgagcagcc ggtcgttgtc gcgctggtgc
      541 aggccgatgg acggctcgtc gagcacatag aggacgccgg tgaggcccga gccgatctgc
      601 gaggcgagac ggatgcgctg gctctcgccg ccggagagcg tgccggagtc gcggttgagc
      661 gtgaggtagg agaggccgac attgacgagg aagccgaggc gctcgcggat ctccttgagg
      721 atgcgctcgg cgatggtcgc ctgcttggcg tccagccgcc cggcgaggtc gtcgaaccag
      781 agggccgcct cctggatgga gagctccgac acggccgcga tgtgccggcc gccgacccgg
      841 accgacagcg cctccggctt gagccgatgg ccctcgcagg cggcgcaggg tgcggacgac
      901 atgtagcgct ggatctcctc gcgcatccag gcgctgtcgg tctcccgcca gcgccgctgg
      961 aggttcaaga tgacgccctc gaaaggcttg ttggtcttca tcgtgcgcaa gccgtcctcg
     1021 aacgacatcg gcacgggctc tttgcctgaa ccgtagagga tggtctgccg ggtctcctcc
     1081 ttgagatcct cccagggcgt gtgcaggctc tcgtcgaaat gccggagcac ggcggcgagc
     1141 gcctgggggt agtagtgcat ggtctgcgac gaccagggct cgaccgcacc ctggtggacg
     1201 gttttcgtcg cgtccggaac gacgagctcc gggtcgacca gcagccgccg cccgaggccg
     1261 tcgcaggacg ggcaggcgcc gtagggattg ttgaaggaga agagccgggg ctcgatctcc
     1321 ggcaaagtga agcccgagac cgggcaggcg aacttggccg agtgcgtcgt gcgctcgccg
     1381 ctgtcggcat cctcgacgac gagcagcccg tcggaaaggc caagcgccgt ctcgatcgaa
     1441 tcggcgagcc gctgggcgag gtcgggggcg gtcaccaggc ggtcgaccac cacctcgatc
     1501 tcgtgcttga gcttgcggtc gagcgccggc gcctcgtcga tctcgtggaa ctcgccgtcg
     1561 atcttgaccc gctggaagcc ttgccgctgc agctccctga gctccttcct gtactcgccc
     1621 ttgcggccgc ggacgatcgg cgccagcaga tagaggcggc tgccctccgg catggcgagg
     1681 acgcgatcga ccatctgcga gaccgtctgc gcctcgatcg gcaggccggt ggccggcgag
     1741 taggggacgc cgacgcgcgc ccagagaagg cgcatgtagt cgtagatctc cgtcaccgtg
     1801 gcgacggtcg agcgcgggtt cttcgaggtg gtcttctgct cgatcgagat cgccggcgac
     1861 aggccctcga tcaggtcgac atccggcttc tgcatcagct cgaggaactg ccgggcgtag
     1921 gccgagaggc tctcgacata gcgccgctgg ccctcggcat agatggtgtc gaaggcgagg
     1981 gaggacttgc ccgagccgga gaggccggtg atcaccacga gccggaagcg cgggatgtcg
     2041 acgtcaacgg acttcaggtt gtgctcgcgg gcaccgcgaa tgcgtatgac gtcgttcatg
     2101 ggcaccgttc gatggcggga tgcggccggg cgcgtgggcg ccgcccatct agttgcgccc
     2161 tgatcgcgaa ttggcaagtg accggcgacc gcatgacact tcggcggcga tgcgtgtata
     2221 ggacgccgag caatcgaacc gtctcggcag gcaggaggaa agcgtcatgg ccggcagcgt
     2281 caacaaggtg atcctggtcg gcaatctcgg ccgcgatccg gaggttcgct acacccagag
     2341 caaccagaag atcgtccatc tggcggttgc cacgtcagag cgctggaagg atcgccagac
     2401 aggggagcag cgcgagaaga cggaatggca ccgcgtggtc atcttcaacg agaacctggc
     2461 cgacgtcgcc gagcgctatc tgcagaaggg acggtccgtc tacctcgagg gccagctcca
     2521 gacgcgcaaa tggaccgacc agtcgggcca ggagaaatac accaccgaga tcgtgctgca
     2581 gcggtttcgg ggcgagctcg tgctgctcgg cggcggtcgc gacgacatgg gctcgggcga
     2641 ccgcgacatg gtcggcggct cctatgacga cccgggcgac cgggcggcac cggcggctcg
     2701 cggcgggcga ccggctggtg gcggccggcc cgcggccgcc gacgatctcg acgacgatat
     2761 cccgttctga accgagccta ggaagaatcc gcgacgcggc ccggcaggta gacgaggaag
     2821 acccgctcgc cctcggcctg ctggttggcc acgttgacgc tcgccccggg aaaatggttt
     2881 cggacgctgg ccgcgaaggc gatcgggttg ggcgcctccg gcagcgggaa ctgcacggcg
     2941 atcaccggtc gatcgcctgt gccgtgccgg ccgaaatcga tgcgctgcgg cagcacgcca
     3001 tgaccggagc aggcagcggc cagccggccg atgcactcgc cgaggctgag cccgccggca
     3061 tagggctcga ggcgggccca ttcgtcgatc ggcaggcgcc gtcgctggcg gccgatccgc
     3121 gccgaccgcg tcagctcggt gcgttgccgc accgtgccgt catgcccgac gagcaggcgc
     3181 tcgaggcgat cgagcgccgc ctgccgctcg gtggcgggcg tgccgtcgtg cacgatcagg
     3241 gcgcgcagct tgtcgagcag gcaggaactg gcggccatcg gcatccggat gcagcgccgc
     3301 gacggtcgcg gccccgatcc tgatacccgg caattcttgc caaacggtga aagactagtg
     3361 gatgtccgcg gcggtggcga aggcggcctt cagggcgtcg atgtcgaaat tgccgctgcg
     3421 cgccgcctcg aggacgggtc gctcgcggcg gatgcagaga tcgatcgccg ccggcagggc
     3481 cgccgcgtca cccgcgggga tgaccacggc gccatgccgg tcggcatgga ccaggtcgcc
     3541 gtgcaccacc ttcatgccgt agatctcgac cggcacctcg atcgcctcga catggacgtc
     3601 ggcatggctc gggccgatct tgccggcgag gagctggaag cgcggatcga tcgcatcgag
     3661 atcgcggatg gagccgttgg tgacgcaccc cagcacaccg aggccgcgat gcacggcggt
     3721 gttcacctcg ccccagaagg cgccgaagcc gggctccgga tcgagatcct ggatgaccgt
     3781 gatgaccggt tgcgggccct tgaccaggta ttcgtagtag ccgatgcgga gcgccttcgc
     3841 ctcggccggc ggcagggggc tcggtgtcgc cgccttgatg gtggccgtcc gggcgaagcc
     3901 gacgatcggc ttcatgtcgg ggaaggcgca gaccatctgg ctgctggtga agccgacggt
     3961 cagccgcttg ccctgcacca cctcgagcgc gttgcagatg gtgggcgtgt cgaaggccgc
     4021 cagatggtcg agcagctcct ggtcgatggt cacgaaccga tttccttccc tgtggccggg
     4081 gcgacccagc gtcgaccgcc gccgtggccc gcatagcacc gctgccggcc ggctggccag
     4141 tgtccggggg cacgatcccc gcaatggcac gtcgacagcg gcgccacggg tcgccatgat
     4201 cacggcagcc atggctcggc tcacccggca ggaatcccat gacgatccag gtcgcgctca
     4261 accatcagac ccgatacggc tatgatcgcc gcatcaatct cgggccgcat gtcgtcaggc
     4321 tgcgccccgc ctcccacagc cgcacccgga tcctgtccta cagcctgaag gtcagcccga
     4381 ccgagcattt catcaactgg cagcaggatc cgcacggcaa ctggctggcg cggctgctgt
     4441 tcaacgagcc gacggaccat ctctcggtcg aggtcgacct cgtggccgag atggcggtcc
     4501 tcaacccgtt cgacttcttc gtcgaggctg ccgccgacac ctggcccttc gcctactcgg
     4561 cgagcctgtt caaggagctg ctgccgttcc tcgagaccga gacgcccggc ccgctcatgg
     4621 ccgagctggt cgcctcgatc ccgcgcgagc cggtcacgac catcgacttc atcgtcgggc
     4681 tgaaccagcg gctgcaggag atgatcggct acctgatccg catggagccg ggcatccagt
     4741 cctgcgagga gacgctggcc caacgctcgg gctcgtgccg cgacacggcc tggctgttcg
     4801 tccagctcct ccgccagctc ggcctcgcgg cgcgcttcgt ctccggctac ctgatccagc
     4861 tcaaggccga ccaggcggcg ctggacggcc cgtcgggcac cgatgtcgac ttcaccgacc
     4921 tgcacgcctg gacggaggtc ttcctgccgg gggccggctg ggtcggcctc gacccgacgt
     4981 cgggcctgct cgccggcgag ggccacattc cgctcgccgc cacgcccgac ccgggcaatg
     5041 ccgccccgat caccggcacg ctcgaccctg ccgaggtctc gttcgacttc tcgatgacca
     5101 tcgagcgcat ccaggagggg ccgcgggtca cactgcccta caccgaggcc gaatgggcct
     5161 cgatcgacgc actcggccac gccatcgacg cccggcttca ggcgcaggac gtccggctga
     5221 ccgtgggtgg cgagccgacc ttcgtcgggc tcgacgaccg cgacggcgac gagtggaaca
     5281 cggcggcggt cgggccgacc aagcggcagt tcgccgacac cctgctgcgc cggctggccg
     5341 accggttcgc gccgggcggt ctcctgcagc atggccaggg caagtggtat ccgggcgagc
     5401 cgctgccgcg ctgggccttt gccgtctact ggcgaacgga tggcgagccg ctctgggccg
     5461 accattcgct gatcgcgtcc gaggaggcgg ctgccgagcg gccgcacgcg gcactggtcg
     5521 aggtcgccga gcggttcacc gcccagctcg ccgccaatct cgagctcgac ccggcccatg
     5581 ttcatccggc ctatgaggac accgcccagt tcgtgctccg cgagcagcaa ttgccggcca
     5641 atgtggaccc gctcgacagc aagctcgagg atgccgagga gcgcgcccgg ctggcgcggg
     5701 tgttcggccg cggcctgacc acgcccacct cgtacgtgct gccgctgcag ccgtggcagg
     5761 ccagggcgag cgccggggcg ccgagccccg gccggcgccg gtttcgctgg atgagcgagc
     5821 gctgggggac gcgccggggc gggctcttcc tgctgcccgg cgacagcccg gcagggttcc
     5881 gcctgccctt gggcgccctc gatcacctgc ggccggacgc ctacccgagc gtcgtgccgc
     5941 tcgacccgtt cgcggcgcac gccgccctgc cggcgcgcga gccggtccag cagccgcgcc
     6001 atgcgcaggg ctacgtgccg gtgcagcccg gtgaccgccc cgtgcccggc gagccggtgc
     6061 gtacggcgct cacggtcgag ccgcgcgagg gccatctcta tgtcttcctg cccccggtcc
     6121 cctcggccga ggagtatgtt gatctcatcg ccgccgtcga ggagacagcg ctggccctgg
     6181 gtcagccgat ccgcctcgaa ggctacccgc cgccagccga cccgcggctg cgcaacatca
     6241 aggtgacgcc cgatccgggg gtgatcgagg tcaatgtcca gccggcggcg agctgggccg
     6301 agcaggtccg gatcaccgag gccatctacg aggaggcccg ccagacccgg ctcgacacgg
     6361 cgaagttcat gctggacggt cggccgaccg ggacaggcgg cggcaatcac atcgtggtgg
     6421 gcgggccgac cgctgccgac agccccttcc tccgtcgacc cgacctcctg gcttcgctga
     6481 tccgctactg gcagaaccac ccctcgctct cctatctgtt ctccggtctg ttcatcggcc
     6541 ccaccagcca ggcgccgagg ctcgacgagg cgaggcagga ccagctctac gagctcgaga
     6601 tagcgctcgc ccgggtgccc gacccgacgc gcggcagcga cctcgactgc ccgccctggc
     6661 tggtcgaccg gctgttccgt catctcctgg tcgacctcac cggcaacacc catcgggccg
     6721 agatctgcat cgacaagctc tacagcccgg acggcccgac cggccggctg gggctcgtcg
     6781 agttccgggc cttcgagatg ccgccgcatc cacgcatgag cctggcgcag aacctgctcc
     6841 tgcgcgccct gatcgcctgg ttctggcagc ggccctacac cggccggctg atccgccacg
     6901 gcaccgacct gcacgaccgg ttcatgctgc cgcactatgt ctgggccgat tttcgctcgg
     6961 tgctggccga cctggaagcg gcgggcatgg gtttcgaccc ggcctggttc gcggcgcacc
     7021 aggaattccg cttcccgctc tacggtcatg tcacggtggc cgatgtcgag gtcgagctgc
     7081 gcgccgcgct cgagccctgg cacgtgctcg gcgaggaggc gaccggcggc ggcaccgcgc
     7141 gctatgtcga cagctcgctc gagcgggtcc aggtgcgggt cacgggtgat cggcagggcc
     7201 gctacgtcgt cacctgcaac ggctacaccc tgccgctcga gccgaccggc gacgggcacg
     7261 aggttgccgg ggtccgcttt cgcgcctggc agccgccctc ctgcctgcac ccgacgatcc
     7321 cggtccacag cccgctcgtc ttcgatgtgg tcgacggggt gcaggggcgc tcgctcggtg
     7381 gctgcaccta tcacgtgatg catccgggtg gtcgctccta cgacacggcg ccggtcaatg
     7441 atttcgaggc acaggggaga aggcttgcgc gctttcaacc catggggcat acgcccggct
     7501 cgatgcgacc gatcgccgtg ccccgcaacc cgagcttccc gcacaccctg gatcttcgcc
     7561 gtgtcggcat ctgaacgagg ggccgcgact tggccctgac cctttcggtg cggaccgaga
     7621 cgatcgacgg gctggtggcc gggtatcggc cgcccggcaa ccagcgggac gagctgctcg
     7681 gcccgggcgg agaggtgcgc aggacctggc agccgttcct cgagcgcttc ggggcgatgg
     7741 gcgccgaagg ccgggacgcg gtggcgcgca ccacggcgca gaagctccag gaaagcggca
     7801 tcgccttcaa cgtgcatgcc gatcccgacg accgcaaggc ggcctggcgg ctcgatctcc
     7861 tgccggtgat cctcgctgcc gacgaatggc aggggctcga gcgcgggctc gtccagcgcg
     7921 cccggctcat cgaggcggtg ctcgccgatc tctacggtgc gcagcgcctc ctgcgcaccg
     7981 gcgtgctgcc gccctcgctg gtgttcggca atccggaatt cctgcacggc tgcgccggct
     8041 ggagcaggcc gccgcgccgc ttcctcttcc attttgcctg cgatgtcgcc cgcgacccga
     8101 atggcggctg gcgcgtgctg gccgaccagg tggatgcccc ggccggcaat ggctggctcc
     8161 tcgccagccg ggtggcgctg agccagggca tgggcgagct ctatctcgaa agcggtgtgc
     8221 ggcgggtcgc cggctactac gcccggatgc aggcggcgct gcaggcggcg gccccggtcg
     8281 acgagggccg gatcgtcacc ctctcgcctg gtgcccgcga tcccggcttc ttcagccacg
     8341 cctatctcgc ccgctatctg ggcctggcgc tggccgaggc tgccgacctc accgagcgtg
     8401 acgggcacgt ctatctcaag acgctggagg gcctgcagcg ggtcgacgtg gtgctgcgca
     8461 agatcggcgg ccgcttcgcc gaccccttga acctctcgac ccggcacgac ggcggcacgc
     8521 ccggcctcgt gcaggcggcc cggctcggcc ggaccttctt cgccaatgcg ctgggcagcg
     8581 gggtcctgca gaaccgcgtg ctcgccgcct tctcgacgtc cctggcggag acgctgctcg
     8641 gcgaggccct cctcctgcgc gagtcgccgg cgctctggct gggcgatgcc ctggcgcagg
     8701 agcgcgtgct cgaggagccg ggctgggtga ccagccggct ggcggcgcgc cacgatcccg
     8761 gcagcagcga tcgccacgac cccctgccgg cgctcgccga cgacgaagcc cggcgccgct
     8821 tcctggcacg cgacggccat ggctgggtcg ccgagcggtc gagcccgctg ccgaccaccc
     8881 cgggcttcca ggagggccgc ctgacgccgg tccactgggc gatgcgcgcc ttcgtctgcg
     8941 tcgacgaggg gggctatcat gtgctgcccg gcgggctcgt gcgccagtcg ccgacgcccg
     9001 ggacgatcgg cctgcccaac ggcttcggct cgaaggatct ctgggtgctg cgcgagccgg
     9061 gcgaggtgct ggcacccagc ctcttcaccc gccgcttcgc ccaggtgcat ctccggcgca
     9121 ccggccgcga tctcctcagt cgcaccgccg aggcgctctt ctggctgggg cgcaatgtcg
     9181 agcgggcgga atcggtcatg cgcacgctgc gcgcggtcct cacgcgcctg atcgacgacg
     9241 gcgggcccga ccggcagccc gacctcctcg aggccctgct cgccatccac ctgggcgagg
     9301 agcgtcccct ggaagggtcg gcgcgcaggc gcatcggcgc cgggctcgag cgcctgctgc
     9361 acgatcccga gcccatgttc ggcgtgcgcc gctccatggc cggcatccgg cacaacgcga
     9421 ccacggcccg ggcggtgctc agccaggacg gctggcgggt tctcaacgcc ctgcacgccg
     9481 accggcgctg gcggtcggcc ggccggggca tcctcgcggc gccgccgatc gacatggtca
     9541 atgacggcat ccgccagctc gtcgccttcg ccggcacggc ggcggagaac atgacgcgca
     9601 actattcatg gcggttcctc gacatgggcc gccggctcga gcgggcgctg cagctcatcg
     9661 acctgatcga agcgctgacc ggcacgctgg acggcatcga cgagggggtg gcgctgactg
     9721 ccctgctcga gatcggcgac agcttcatga cctatcgctc gcgctatgcg gtgacaccga
     9781 tggcgacccc ggtggtcgat ctcctggccc tcgacgagac caacccgcgc tcgctcgcct
     9841 accagctcct gacgctcgag ggccacctgg cgagcctgcc gagcgagggg ccctatcggt
     9901 cggccgagca gcgccgggtg ctctccctcc tcaccgagct gcagctcgcc gatgccgacc
     9961 agctcaccgc cgaccgggag cagggggcgg cgagcgtgct ggccggattc ttcgcccgcg
    10021 cccgggtgga cctgcgcgag gccgccgacc tgatcgggcg tggctatttc gtcctggccg
    10081 agacgccgac gagcacgctg gcgatgcgcc gcgtcgaggg gctcggaggg gtcggcccat
    10141 gatcttcgac gttcgccatc gtaccgacta cagctacagc cagctcgtct cgatctcgca
    10201 tcacctgctg catctcgagc cgcgcgacac gccgcaccag cggatcgagc gcttcgagct
    10261 gagcatcgag ccgagcccgg gcttcgacga tcgtgacgtc gataccttcg gcaacccgac
    10321 ccgcaccatc accctcgacc agaaccacga cgagctggtg atcgaggccc gcagccgggt
    10381 ccggctggcg gagcgcgagg tgccgccccc cctggatacg ccggcctggg aggaggcggc
    10441 cgcggtgcgc ctcgccgcca ccgacgacga tgcgctggag gccagccgct acgctttcgc
    10501 ctcgccgttc gtcgatctcg gcgatgcggt cgaggcctat gcccgagagt gcttcctgcc
    10561 gggtcggccg atcctcgatg cggcgctcga gctcaatggc cggatctttc gcgagttcac
    10621 cttcgacaac aaggcgacgt cgctggcgac gccggtcgcc gaggtgctcg agacgcgacg
    10681 cggggtctgc caggatttct cccatctgat gctcgcctgc ctgcgctcgc tcgggctgcc
    10741 ggcgcgctac cagtcgggct acatcatgac ccgtccgccc gagggccagg aacggctggt
    10801 cggcgccgat gccagccatg cctgggtggc gctctgggtg ccccgccacg gctgggtcga
    10861 gctcgatccg accaatgact gcctgccgat gcgcgagcac gtgacgctgg cctgggggcg
    10921 cgactacggc gatgtcagcc cgatctccgg catcgtctat ggcggtggcg cgcacgatct
    10981 gacggtcgcc gtggacgtcg tgccgctcgt tccggcataa ggtcgccggg atggaggtcg
    11041 acttcatcgt cgtcggagcc ggcagcgccg gctgcgccct ggccagccgg ctcgccgagg
    11101 acgggcggag cacggtcctc gtgctcgagc atggcggcac cgatgccggc ccgctcatcc
    11161 agatgccggc ggccctctcg atcccgatga acatgcgccg ctacgactgg ggctaccgga
    11221 ccgagccgga gcccgggctc gacggccggc ggctggcctg cccgcgcggc aaggtaatcg
    11281 gcggctcctc ctcgatcaac ggcatggtct at
//
LOCUS       DAOTFI010000165        11172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6938_length_11172_cov_8.677161, whole genome shotgun sequence.
ACCESSION   DAOTFI010000165 DAOTFI010000000
VERSION     DAOTFI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6938_length_11172_cov_8.677161"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..825)
                     /locus_tag="P5558_16035"
     CDS             complement(1..825)
                     /locus_tag="P5558_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY25879.1"
                     /translation="MSYRTMLLQMFEDAALTRRLTTAVDLARQFQGHLTGLNVAPPPV
                     MPFGYGEAAAYVGPEIFEAQRQANEAIARRLEAAFGKATSGQGIAADWRSLDGDYVTA
                     FGKAAHTADLVITGQVPGDALDALAPQVTEFLVTEAGGPVLMLPASGSGGSIGQRIIL
                     GWNGSKEAKRAVLGSLPFLRGATQVWLVTVGDPEAVRLDAARTMLERHDIRVEPVVIQ
                     GSGQEAGAILLEQAQSRDADLVVMGAYGHSRLREFFLGGATRHVLQTSALPILFGG"
     gene            complement(905..1525)
                     /locus_tag="P5558_16040"
     CDS             complement(905..1525)
                     /locus_tag="P5558_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY25880.1"
                     /translation="MLDRFARPLIDPPLDRLAARLAPRVAADEVTLVGFALGLVAMAL
                     IMLGLHLPGLAFLLLNRLADGLDGAIARRNGITDLGGYLDITLDLIVYSGLVFAMAVA
                     DPAANALAAAFLVFSFMGTGASFLAFAIMAARRGLESRERGTKSLYFLGGLAEGTETI
                     LFFVLALLWPAAFAGLAWAFGLVCWVTTASRILMARQLLGDRPAGG"
     gene            complement(1536..2408)
                     /gene="pssA"
                     /locus_tag="P5558_16045"
     CDS             complement(1536..2408)
                     /gene="pssA"
                     /locus_tag="P5558_16045"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRY25881.1"
                     /translation="MAGKFRRRRLRPRPVLHFVPNMFTILGLCAGMTGIRYAFDGRWQ
                     LAVGFILAAAVFDGLDGRSARLLKMTSKLGEQLDSLADFLSFGVAPAIIVYLWVLHDV
                     RGLGWAVALVFATCSALRLARFNAELDAPERPRWALYFFTGVPAPAAAGLALLPIMAS
                     FVIGSELVRAWWLNGLVLVTVALLMVSRVPTFSVKRLRIKPALVIPTMILGVFVAVLL
                     VTEVWLMLTLVGLAYFISIPVSWRVAARMERDSDAALPEPEPVAAEDVAGGTTETART
                     DPAVYRLEGRHKQP"
     gene            complement(2444..3145)
                     /locus_tag="P5558_16050"
     CDS             complement(2444..3145)
                     /locus_tag="P5558_16050"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRY25882.1"
                     /translation="MAEPGSLGSIIAPIHKAGWPFIAIFFAVAVVLGFLWQPLFWLGL
                     VATLWCVFFFRDPPRTTPIAPGLVTSPCDGMVAATTPRVPPPELGLGSEPLPCIGIFM
                     NVFDVHVNRTPVPGRVLARAYHQGKFVNASFDKASDENERQSFRIETADGSEVGIVQI
                     AGLVARRIIAFVEEGAALAPGQRIGLIRFGSRCDVYLPRHVQPVVLPGQRAIGGETVI
                     AHLDGPQTPRLGRTD"
     gene            complement(3171..3878)
                     /locus_tag="P5558_16055"
     CDS             complement(3171..3878)
                     /locus_tag="P5558_16055"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRY25883.1"
                     /translation="MSPAEKPRDGPARLACLLARQRIDAVLDVGANAGQYGTMLRQAG
                     YGGPILSFEPLAEPRAALARAAGEDPLWRLAPPLALGDRAGEATIDVSAESDMSSILP
                     QAAMLRDLSPSSAVLERRRVTTARLDARETLLDPAWRRMHLKIDVQGYEPQVLDGAAG
                     LMDRIATLQLELALEPVYVGETGWRAMVDRLTEAGFRLALVLPGYFERKLGRMLQFDG
                     VFIRHDAGDPAADHLPN"
     gene            complement(3875..5131)
                     /locus_tag="P5558_16060"
     CDS             complement(3875..5131)
                     /locus_tag="P5558_16060"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY25884.1"
                     /translation="MSTDPAESGHWPSRRSPVLDWLSTGVIDQPLLDPLFADLCRKLR
                     AHGLPLDRATLHLRILHPQFGGATIRWTAEPDELQLTTVDREVWRNRRYLESPLRPLY
                     EGEVDGIRARLDLPQPPGAPVFAIYDELRAEGIVDYVVLPMEFVSGTRHACTWSTKQA
                     GGFTTDDLKAIADLLPVLAMAAEIRLTRRITRNLLDTYVGRRSGQRILEGQIDRGSGE
                     TLSAAIWHCDLRGFTMLSERRPRDDVIQSLNAYFDVMAEPLTRQGGEILKFIGDAMLA
                     IFPMAEPRACQRALRAALEAQEGMARLNEERRAVGQAELGFGLALHVGDVMWGNIGAQ
                     ERLDFTVIGPAVNVATRLEELSKELRRPLLVSGDFAGACACAHATLDRLGDYALRGVE
                     REVTVHAPRPDALERLRAAEAALPPA"
     gene            complement(5128..5601)
                     /locus_tag="P5558_16065"
     CDS             complement(5128..5601)
                     /locus_tag="P5558_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY25885.1"
                     /translation="MTLQKDVEALRNIALFRKIEPAKLKLLAFTSEHLEFMPGETLFH
                     QGDVGDAAYILLEGDADVMVETPNGALKVATLHQNDIVGEIAILCDVPRTASVVAANK
                     LVTLRVSKDGFFNLVTQFPQVGVEIMHELASRLHHTTQQLGDATAKLRTMQTGAG"
     gene            complement(5603..8272)
                     /locus_tag="P5558_16070"
     CDS             complement(5603..8272)
                     /locus_tag="P5558_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25886.1"
                     /translation="MEPTIFGFIRRYSWRQQLIILGMTVLSFPFLYASLDLPKQIIND
                     ALGQADGGTVLGFQLDQLGYLFALCGIFLVLVLINGAFKYVINVYSGVVAERMLRRLR
                     YMLYNHMLRFPLAHYRRMTQGELVQMINAEVEPLGGYVGDALSTPAFQGGTLVTILAF
                     MFMQDPVLGLAAVALYPLQIYLIPKLQRQVNELGKRRVRQVRRLAERISETAQGVRDI
                     RANDATQYQRAVFTRELGHVFDIRFEIYKKKFFIKFLNNFLAQLGPFFFFTIGGYLVI
                     QGDLTIGALVAVVGAQKDLSSPWRELLAHYQLFMDVRIKYDQVIAQFSIPGLIDERRQ
                     DDDNPGDTPLTGAWHARGVTVIDEDDETNLESISFELDLPQHVAIVGPSGSGKEELCL
                     VLANLIDPSRGRLLVNGTNLADLAESLTGRRIAYVGNPSAILAGTIRHNLLYGLMHRP
                     MHDVVLDGETAAARERRYKENQLSGNSPYDSDADWIDYSPLGEAAENDDARRAAIAEC
                     LRVVGFDRDVYVMGLRSSIDETIDADLPQALLQARKAMQARLDEDSDLARLVERFDPE
                     RYNTNATVAENLLFGTPVGRTFDLDSLAEQPYVRAVLDKTGLTEELVAAGYKLAATMV
                     EIFADLPPDHEYFRQFSFIDAEALPGYRALLNRADPSRLDQLGKDDRQLLLALPFKLI
                     PARHRLGMVEDDLRQRLLEARRVFREELPASLEGAVAFFDPDAYNEAASIQDNILFGK
                     IAYGQAQAESRIAALITEVLEGQGLIDRVVEVGLSSPAGYGGARLSPVQRQKLALARA
                     LMKRAEIYVFDDPLAPFDSREQALVRGALLEYLAGKSVFWALQNAEWARHFDHVLVLD
                     QGRLVEHGAYEKLATRGHALHRLVSAHEESGSS"
     gene            8455..10374
                     /locus_tag="P5558_16075"
     CDS             8455..10374
                     /locus_tag="P5558_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HRY25887.1"
                     /translation="MQGIYRQIYERSLERPDEFWAEAAEALHWDRRWDRVLDDSRAPF
                     YRWFAGGRLNTCYNAVDRHVRAGRGAQPAIIHDSPVTGTKRVITYGELLDLVARFAGA
                     LVQRGVRHGDRVIIYMPMVPEALVAMLACARIGAIHSVVFGGFAAHELATRIDDCKPV
                     MIVAASCGIEPGRVVQYKPLIDRAIEEAAHKPRHCIVLQRPEATAALTAGRDLDWQAA
                     MAAAHPVDCVAVEATDPLYILYTSGTTGQPKGVVRDHGGHATALLWSMRHIYGMQPGE
                     VFWAASDVGWVVGHSYICYAPLLNGSTTIVYEGKPVGTPDAGAFWRVIAEHKVAAMFT
                     APTAFRAIRKEDPEGEHVRRYDLAHFRTLFLAGERCDPDTLLWAEAQLGVPVIDHWWQ
                     TETGWAIAANPAGVELLPVKPGSPTLPMPGYDVRILDEEGQEVSGPGTIGAVVVKLPL
                     PPSCLPTLWQADDRYRQAYLSRYPGYYLTGDAGYVDADGYVFIMSRIDDIINVAGHRL
                     STGGMEEVLAGHPAVAECAVIGVADTLKGELPMGFVVLKSGVTKPEAEVMKELVAKVR
                     DEIGPVAAFKLVAVVKRLPKTRSGKILRGIMKKIADDMPFNMPATIDDPAILDEIAEA
                     LQGLGYANAKHDRDR"
     gene            10349..10552
                     /locus_tag="P5558_16080"
     CDS             10349..10552
                     /locus_tag="P5558_16080"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRY25888.1"
                     /translation="MRSTIEIDEALMRRAMAASGHSTKRATVEAAPTLLVRLKGQERL
                     RDPFGKLPWDDDPGAMRNDRAPG"
     gene            complement(10558..>11172)
                     /locus_tag="P5558_16085"
     CDS             complement(10558..>11172)
                     /locus_tag="P5558_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY25889.1"
                     /translation="LNRESGGFCTAVLIGPKEALTAAHCLWDQSQRRWVQPSTVHFVP
                     GYRRGTYLGHSRGKAFRLADGIAIDATGHPKNLLDDWAVIELDLDLSAGTGVQPMPLA
                     DASERDRLAGNPIARGGYSSDRPHLPAKVDPCKALAVIEQGRLLLHDCDATAGSSGSP
                     LVIERDGRLLVVGIQSAVVRSGDEAVAVGILMQRVLPAAALVSQ"
BASE COUNT         1705 a         3592 c         4267 g         1608 t
ORIGIN      
        1 tcagccgccg aacaggatcg gcagggcgct ggtctgcagg acatggcggg tggcgccgcc
       61 gaggaagaac tcgcgcagcc gggaatggcc gtaggcgccc atcaccacga ggtcggcgtc
      121 gcggctctgc gcctgctcca gcaggatggc accggcttcc tggcccgagc cctggatgac
      181 cacgggctcg acccggatgt cgtgccgctc gagcatggtc cgtgcggcgt cgagccggac
      241 ggcctccggg tcgcccaccg tgaccagcca gacctgggta gcgccccgca ggaaggggag
      301 gctgccgagc accgcccgct tggcctcctt cgagccgttc cagccgagga tgatccgctg
      361 gccgatgctg ccgccgctgc cgctcgccgg cagcatcagg accgggccgc cggcttcggt
      421 gacgaggaac tcggtgacct gtggcgccag cgcgtccagg gcgtccccag ggacctggcc
      481 ggtgatgacg agatcggcgg tgtgggccgc cttgccgaag gcggtgacgt agtcgccgtc
      541 caggctgcgc cagtccgcgg cgatgccctg gcccgacgtg gccttcccga aggctgcctc
      601 gaggcgccgg gcgatggcct cgttggcctg gcgctgggcc tcgaagatct cgggcccgac
      661 ataggccgcg gcctcgccat agccgaacgg catgaccggc ggcggcgcca cgttgaggcc
      721 ggtgaggtgg ccctggaact ggcgggcgag atcgacggcc gtggtgagcc ggcgcgtcag
      781 cgcggcgtcc tcgaacatct gcagcaacat cgtgcgatag ctcatgaagt cacctcgctt
      841 gcccggccgt tcgtcccatg gctcgaagtc taggccctgt gcccgcctcc tgtcatgatc
      901 tggatcaacc gcccgccggc cggtcgccca ggagctgccg cgccatcagg atgcggctcg
      961 ccgtggtgac ccagcagacg agcccgaagg cccaggcgag cccggcgaag gcggccggcc
     1021 agagcagggc cagcacgaag aagaggatcg tctccgtgcc ctcggcgagc ccgcccagga
     1081 agtagagcga cttggtgccc cgctcgcggc tctcgaggcc gcgcctggcc gccatgatgg
     1141 cgaaggcgag gaaggaggcg ccggtgccca tgaaggagaa gacgaggaag gcggcggcca
     1201 gcgcgttggc ggcgggatcg gccaccgcca tggcgaagac gaggcccgag tagacgatga
     1261 ggtcgagggt gatgtcgaga tagccgccga gatcggtgat cccgttgcgg cgggcgatcg
     1321 ccccgtcgag cccgtcggcg aggcggttga ggaggaggaa ggcgaggccc ggaagatgga
     1381 ggccgagcat gatcagggcc atggcgacga gcccgagggc gaagcccacc agggtcacct
     1441 catcggcggc gacgcgcggg gcgagccgtg ccgcgaggcg gtcgagcggc gggtcgatca
     1501 gcgggcgcgc gaaccggtcg agcacggcgc caggctcagg gctgcttgtg ccggccttcg
     1561 aggcgataga cggcggggtc cgttcgggcg gtttcagtcg tcccgcccgc gacatcctcg
     1621 gccgcgaccg gctccggctc gggcagggcc gcgtcgctgt cgcgctccat ccgcgctgcc
     1681 acgcgccagc tcaccgggat ggagatgaag taggcgaggc cgaccagggt cagcatcagc
     1741 cagacctcgg tgacgaggag gacggcgacg aagacgccga ggatcatggt cgggatgacc
     1801 agggccggct tgatccgcag ccgcttgacc gagaaggtcg gcacgcggct gaccatcagg
     1861 agcgctacgg tgaccagcac gaggccgttc agccaccatg cgcggacgag ctcgctgccg
     1921 atgacgaagg acgccatgat cggcaagagc gccagcccgg cggcggccgg ggccggcacg
     1981 ccggtgaaga agtagagggc ccagcgcggc cgctcgggcg catcgagctc ggcattgaag
     2041 cgggcgaggc gcagcgccga gcaggtggcg aagacgaggg cgacggccca gccgaggccg
     2101 cgcacgtcgt gcagcaccca gagatagacg atgatcgccg gcgccacgcc gaagctgagg
     2161 aagtcggcga ggctgtcgag ctgctcgccg agcttggagg tcatcttgag cagccgggcg
     2221 gagcggccgt cgagcccgtc gaacacggcg gccgccagga tgaagccgac ggcgagctgc
     2281 cagcggccgt cgaaggcgta gcggatgccg gtcatcccgg cgcagaggcc gaggatggtg
     2341 aacatgttgg ggacgaagtg gaggaccggc cgcggccgga ggcgccggcg acggaacttt
     2401 ccagccattc gcggaccggt cggcggcggg ctctggctgg ccatcagtcg gtgcgtccca
     2461 ggcgcggggt ttgcgggccg tcgaggtggg cgatcaccgt ctcgccgccg atcgcccgct
     2521 ggccgggcag caccaccggc tgcacgtggc gcggcaggta gacgtcgcag cggctgccga
     2581 agcggatcag gccgatgcgc tggccggggg cgagggccgc accctcctcg acgaaggcga
     2641 tgatgcgccg ggcgacgagg ccggcgatct ggacgatgcc gacctcgctg ccgtcggcgg
     2701 tctcgatgcg gaagctctgg cgctcgttct cgtccgacgc cttgtcgaag gaggcgttca
     2761 cgaacttgcc ctggtggtag gccctggcca ggacgcggcc gggcaccggc gtgcggttca
     2821 catggacgtc gaagacattc atgaagatgc cgatgcaagg caagggctcg ctgccgagcc
     2881 cgagctccgg cggtggcacg cgcggtgtgg tggccgcgac catgccgtcg cacgggctgg
     2941 tcacgagccc cggtgcgatg ggcgtggtgc gcggcgggtc gcggaagaag aagacgcacc
     3001 agagcgtcgc cacgaggccc agccagaaga gcggctgcca gaggaagccg agcaccaccg
     3061 cgacggcgaa gaagatcgcg atgaacggcc agccggcctt gtggatcggg gcgatgatgc
     3121 tgcccaggct gccgggctcc gccatggaat tcctttgcgc ggtgtgatgc tcagttaggt
     3181 aggtggtccg ctgccggatc gccagcgtcg tggcggatga agacgccgtc gaactgcagc
     3241 atgcggccga gcttgcgctc gaagtagccg ggcagcacga gggcgaggcg gaagccggcc
     3301 tcggtcagcc ggtcgaccat ggcgcgccag ccggtctcgc cgacatagac gggctcgagc
     3361 gccagctcga gctgcagggt ggcgatgcgg tccatgaggc cggcggcacc gtccagcacc
     3421 tgcggctcgt agccctgcac gtcgatcttg agatgcatcc gccgccaggc ggggtcgagg
     3481 agggtttccc gggcgtcgag gcgggcggtc gtcacccggc ggcgctcgag cacggccgag
     3541 ctcggcgaga gatcgcgcag catcgccgcc tgcggcagga tcgagctcat gtcgctctcg
     3601 gccgagacgt cgatggtggc ctcgccggcg cggtcgccga gcgccagcgg cggggcgagg
     3661 cgccagagcg ggtcctcgcc ggccgcccgg gccagcgccg cgcgcggctc ggccaggggc
     3721 tcgaacgaga ggatcgggcc accatagccg gcctgacgca gcatcgtgcc gtactggccg
     3781 gcattggcgc cgacatcgag caccgcgtcg atccgctggc gcgccagcag gcaggcgagc
     3841 cgggccgggc cgtccctggg tttttcggct ggactcatgc gggcggcagg gctgcctcgg
     3901 ccgcccgcag gcgctcgagc gcgtcgggcc tgggggcgtg gacggtgacc tcgcgctcga
     3961 cgccacgcag cgcgtagtcg ccgaggcggt cgagggtcgc gtgggcgcag gcgcaggcgc
     4021 cggcgaagtc gccggagacg agcaggggcc ggcgcagctc cttggagagc tcctcgagcc
     4081 gggtggccac gttgacggcc gggccgatga cggtgaagtc gagacgctcc tgggcgccga
     4141 tattgcccca catcacgtcg cccacatgca gcgccaggcc gaagccgagc tccgcctggc
     4201 cgacagcacg gcgttcctca ttgaggcggg ccatgccttc ctgggcctcg agcgccgccc
     4261 tgagagcgcg ttggcaggcg cgtggctcgg ccatcgggaa gatcgccagc atggcgtcgc
     4321 cgatgaactt caggatctcg ccgccctggc gggtcaaggg ctcggccatg acgtcgaaat
     4381 aggcattgag cgactggatg acgtcgtcgc gcggccggcg ttcggagagc atggtgaagc
     4441 cgcgcaggtc gcagtgccag atggcggcgg acagcgtctc gccgctgccg cggtcgatct
     4501 ggccctcgag gatccgctga cccgagcgtc ggccgacata ggtgtcgagc aggttcctgg
     4561 tgatccggcg cgtgaggcgg atctcggccg ccatggcgag caccggcagg aggtcggcga
     4621 tggccttcag atcgtcggtc gtgaagccgc cggcctgctt ggtgctccag gtgcaggcat
     4681 ggcgggtgcc gctgacgaac tccatcggca gcacgacgta gtcgacgatt ccctcggccc
     4741 tgagctcgtc atagatggcg aagaccggtg ctcccggcgg ctgcggcagg tcgaggcggg
     4801 cgcggatgcc gtcgacctcg ccctcgtaga gcgggcggag cgggctctcc agatagcgcc
     4861 gattccgcca gacctcgcgg tcgacggtgg tgagctggag ctcgtccggc tcggccgtcc
     4921 agcggatcgt ggcgccgccg aattgcgggt gcaggatgcg caggtgcagg gtcgcccggt
     4981 cgagcggcag gccatgggcg cgaagcttgc ggcagagatc ggcgaagagg gggtcgagca
     5041 gcggctggtc gatgaccccg gtcgagagcc agtcgaggac ggggctgcgc cggctcggcc
     5101 agtggccgga ttccgccggg tcggtgctca tccggcgccg gtctgcatgg tgcgcagctt
     5161 cgccgtggcg tcgccgagct gctgggtcgt gtggtgcagg cgcgacgcca gctcgtgcat
     5221 gatctcgacg ccgacctgcg ggaactgcgt cacgaggttg aagaacccgt ccttggagac
     5281 ccggagcgtg acgagcttgt tggccgcgac cacgctcgcg gtgcgcggca cgtcgcagag
     5341 gatggcgatc tcgccgacga tgtcgttctg gtgcagggtg gcgaccttga gggccccgtt
     5401 gggcgtctcg accatcacgt cggcgtcgcc ctcgagcagg atgtaggcgg cgtcgccgac
     5461 atcgccctgg tggaacagcg tctcgcccgg catgaactcg aggtgctcgc tggtgaaggc
     5521 caggagcttg agcttggcgg gctcgatctt gcggaacagc gcgatgttcc gcagcgcttc
     5581 gacgtccttc tgcaaggtca tgtcacgagc tccctgactc ttcgtgcgcg ctgacgaggc
     5641 gatgcagcgc atgtccccgg gtggcgagct tctcgtaggc gccgtgctcg accagccggc
     5701 cctgatccag cacgagcacg tggtcgaagt ggcgcgccca ttcggcgttc tgcaacgccc
     5761 agaagacgct tttgccggca aggtattcga gcagcgcgcc gcggaccagc gcctgctcgc
     5821 gcgaatcgaa gggggcgagc gggtcgtcga agacgtagat ctcggcgcgc ttcatcaggg
     5881 cgcgggcgag cgccagcttc tggcgctgca cgggcgagag gcgggcgccg ccatagccgg
     5941 cgggcgagga gaggccgacc tcgaccaccc ggtcgatcag gccctggccc tccagcacct
     6001 cggtgatcag ggcggcgatg cggctctcgg cctgggcctg gccgtaggcg atcttgccga
     6061 agaggatgtt gtcctggatc gaggccgcct cgttgtaggc gtcggggtcg aaaaaggcga
     6121 ccgcaccctc gaggctcgcc ggcagctcct cgcggaagac gcggcgcgcc tccagcagcc
     6181 gctggcgcag gtcgtcctcg accatgccga ggcggtggcg ggccgggatc agcttgaacg
     6241 gcagggccag caggagctgc cggtcgtcct tgccgagctg gtcgagcctc gatgggtcgg
     6301 cgcggttgag cagggcccgg tagcccggca gcgcctcggc gtcgatgaag ctgaactggc
     6361 ggaaatactc gtggtccggc ggcaggtcgg cgaagatctc gaccatggtg gcggccagct
     6421 tgtagccggc ggcgaccagc tcctcggtga gcccggtctt gtcgagcacg gcccgcacat
     6481 agggctgctc ggcgaggcta tcgaggtcga aggtcctgcc gaccggggtg ccgaagagca
     6541 ggttctcggc gactgtcgca ttggtgttgt agcgctcggg gtcgaagcgc tcgacgaggc
     6601 gggcgaggtc gctgtcctcg tcgagccgcg cctgcatggc cttgcgggcc tggaggagcg
     6661 cctgcggcag gtcggcgtcg atcgtctcgt cgatgctgct gcgcagcccc atgacgtaga
     6721 catcgcggtc gaagccgacg acccgcaggc attcggcgat ggcggcgcgg cgggcgtcgt
     6781 cgttctcggc cgcctcgccg agcggcgagt agtcgatcca gtcggcgtcg ctgtcgtaag
     6841 gcgagttgcc cgaaagctgg ttctccttgt agcgccgctc ccgcgccgca gcggtctcgc
     6901 cgtcgaggac gacatcgtgc atcggccggt gcatcagccc gtagagcagg ttgtggcgga
     6961 tcgtgccggc caggatggcc gaggggttgc cgacataggc gatgcggcgg ccggtgagcg
     7021 attcggcgag gtcggcgagg ttggtgccgt tgaccaggag ccggccgcgg ctcgggtcga
     7081 tcaggttggc gaggacgagg cagagctcct ccttgccgct gcccgagggg ccgacgatcg
     7141 ccacatgctg cggcaggtcg agctcgaagc tgatggattc gaggttggtc tcgtcgtcct
     7201 cgtcgatgac ggtgacgccg cgcgcgtgcc aggcgccggt gagtggggtg tcgccggggt
     7261 tgtcgtcgtc ctggcggcgc tcgtcgatca gccccgggat gctgaactgt gcgatcacct
     7321 ggtcgtactt gatgcggacg tccatgaaga gctggtagtg ggcgagcagc tcccgccagg
     7381 gcgacgacag gtccttctgc gcgccgacca cggcgaccag ggcgccgatg gtcagatcgc
     7441 cctggatgac caggtagccg ccgatggtga agaagaagaa ggggccgagc tgggcgagaa
     7501 aattgttgag gaacttgatg aagaacttct tcttgtagat ctcgaagcgg atgtcgaaga
     7561 cgtggccgag ctcgcgggtg aagacggcgc gctggtactg ggtggcgtcg ttggcgcgga
     7621 tgtcgcgcac cccctgggcg gtctcgctga tccgctcggc gaggcggcgg acctgccgga
     7681 cccggcgctt gccgagctcg ttgacctgcc gctgcagctt cgggatcagg tagatctgga
     7741 gcgggtagag cgccaccgcg gcgagcccga gcaccgggtc ctgcatgaac atgaaggcga
     7801 ggatggtgac gagcgtgccg ccctggaagg cgggggtgga gagcgcgtcg ccgacatagc
     7861 cgcccagggg ctcgacctcg gcattgatca tctgcaccag ctcgccctgg gtcatgcggc
     7921 ggtagtgggc cagggggaag cgcagcatgt ggttgtagag catgtagcgc aggcggcgca
     7981 gcatccgctc ggcgaccacg cccgagtaga cgttgatgac gtatttgaag gcgccgttga
     8041 tcagcacgag cacgaggaag atgccgcaga gggcgaagag atagccgagc tggtcgagct
     8101 gaaatcccag gacggtgccg ccatcggcct ggccgagggc gtcgttgatg atctgcttgg
     8161 gcaggtcgag ggaggcgtag aggaacggga acgacagcac cgtcatcccg aggatgatca
     8221 gttgctgacg ccagctgtag cgtcgaatga agccgaagat cgtgggttcc atgccacccc
     8281 tgcgctgccc gtcgccggcc cgccggcgcg gaccttagca ggctgccggc gatgccgcga
     8341 tgggcagggg tgctgccggg ggcggtattt cgccaccttg tcgacggccg ggacccgccg
     8401 catgctgacg ggaatggcat ccggtcgcac gccggtcaga acgcggggga gagcatgcag
     8461 ggcatctacc ggcagatcta cgagcgcagc ctcgagcggc ccgacgagtt ctgggccgag
     8521 gcggccgagg cgctgcactg ggaccgcagg tgggaccggg tgctcgacga ttccagggca
     8581 cccttctatc gctggttcgc cggcggccgg ctcaacacct gctacaacgc ggtcgaccgc
     8641 cacgtgcgtg ccggccgggg tgcccagccc gcgatcatcc acgacagccc ggtgaccggg
     8701 accaagcggg tgatcaccta cggcgagctc ctggacctcg tcgcccgttt cgccggtgcc
     8761 ctcgtgcagc ggggcgtgcg gcacggtgac cgggtgatca tctacatgcc gatggtgccc
     8821 gaggcgctgg tggcgatgct cgcctgcgcc cggatcggcg ccatccactc ggtggtgttc
     8881 ggcggcttcg ccgcgcacga gctggcgacg cggatcgacg attgcaagcc ggtgatgatc
     8941 gtggcggcga gctgcggcat cgagccgggc cgggtcgtcc agtacaagcc gctgatcgat
     9001 cgggcgatcg aggaggcggc gcacaagccc cggcactgca tcgtgctgca gcgaccggag
     9061 gcgacggcgg cgctgacggc ggggcgggat ctcgactggc aggcggcgat ggcggcagcg
     9121 caccccgtcg actgcgtggc ggtggaggcg accgacccgc tctacatcct ctacacctcg
     9181 ggcacgaccg gccagcccaa gggcgtggtg cgcgaccatg gcggccacgc gacggcgctg
     9241 ctctggagca tgcgccacat ctacgggatg cagccgggcg aggtcttctg ggccgcctcg
     9301 gatgtcggct gggtcgtcgg ccacagctac atctgctatg cgcctttgct caacggcagc
     9361 accaccatcg tctacgaggg caagccggtc ggcacccccg atgccggcgc cttctggcgg
     9421 gtgatcgccg agcacaaggt ggcggcaatg ttcaccgccc ccaccgcctt tcgggcgatc
     9481 cgcaaggagg acccggaggg tgagcatgtc cgccgctacg atctcgcgca tttccgcacg
     9541 ctcttcctcg ccggcgagcg ctgcgacccg gacacgctgc tctgggcgga ggcgcagctc
     9601 ggcgtgccgg tgatcgacca ctggtggcag accgagaccg gctgggcgat cgccgccaac
     9661 ccggccgggg tcgagctgct gccggtcaag ccgggctcgc cgacgctgcc gatgccgggc
     9721 tacgacgtgc gcatcctcga cgaggagggg caggaggtga gcgggcccgg cacgatcggc
     9781 gccgtcgtcg tcaagctgcc gctgccgccc tcctgcctgc cgaccctctg gcaggccgac
     9841 gaccgctacc ggcaggccta tctcagccgc tatccgggct actacctcac cggcgatgcc
     9901 ggctatgtcg acgcggacgg ctacgtcttc atcatgagcc gcatcgacga catcatcaac
     9961 gtggccggcc accggctctc gaccggcggc atggaggagg tgctggccgg gcatccggcg
    10021 gtggccgagt gcgcggtgat cggcgtcgcc gacacgctca agggcgagct gcccatgggc
    10081 ttcgtcgtcc tcaagtccgg cgtgaccaag cccgaggcgg aggtgatgaa ggagctggtc
    10141 gccaaggtcc gcgacgagat cggcccggtg gcggcgttca agctggtggc cgtggtcaag
    10201 cgcctgccga agacgcgctc cggcaagatt ctccgcggca tcatgaagaa gatcgccgac
    10261 gacatgccct tcaacatgcc ggcgacgatc gacgacccgg ccatcctgga cgagatcgcc
    10321 gaggcgctgc aggggctggg atacgccaat gcgaagcacg atcgagatcg atgaggctct
    10381 gatgcggcgg gcgatggccg cgagcgggca ctcgaccaag cgtgccaccg tcgaggcggc
    10441 gccgacgctg ctcgtgcggc tcaagggcca ggaacgcttg cgcgacccct tcggcaagct
    10501 gccgtgggac gatgacccgg gtgcgatgcg aaatgatcgc gccccggggt agtgccgcta
    10561 ctggctgacg agggccgccg ccgggaggac gcgttgcatg aggatgccga cggcgaccgc
    10621 ctcgtcgccg gagcggacca cggccgactg gatgccgacg acgagcaggc ggccgtcgcg
    10681 ctcgatcacg agtggcgagc cggaggagcc ggcggtggcg tcgcagtcgt gcagcaggag
    10741 gcggccctgc tcgatcacgg cgagcgcctt gcaggggtcg accttggccg gcagatgcgg
    10801 ccggtcgctg ctgtagccgc cgcgggcgat ggggttgccg gcgaggcggt cgcgctcgct
    10861 ggcgtcggcg agcggcatgg gctgcacgcc ggtgccggcg ctcaggtcga gatcgagctc
    10921 gatcaccgcc cagtcgtcga ggaggttctt cggatggccg gtggcgtcga tggcgatgcc
    10981 gtcggcgagg cgaaaggcct tgccccggga gtggccgaga taggtgccgc gccggtagcc
    11041 gggcacgaaa tggacggtgc tcggctgcac ccagcggcgc tgcgactggt cccagaggca
    11101 atgggcggcg gtcagcgctt cctttgggcc gatcagcacg gcggtgcaga agccgccgga
    11161 ttcgcggttg ag
//
LOCUS       DAOTFI010000166        11105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_6986_length_11105_cov_6.495294, whole genome shotgun sequence.
ACCESSION   DAOTFI010000166 DAOTFI010000000
VERSION     DAOTFI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6986_length_11105_cov_6.495294"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="P5558_16090"
     CDS             <1..1377
                     /locus_tag="P5558_16090"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY25890.1"
                     /translation="LLALGAAGPTWSRMPDPFVAQSAPVVVVLEVTPSMEAKDIAPSR
                     LERGKQKIRDLLELRAGARTALVAYAGSAHGVVPMTEDPAVMTPYLAGLAPDVMPNEG
                     ERAGDALALAVDLLAQESSPGGVLFVADGVDPADVATLTGPDAPALAVLAMLPEGTSD
                     RGLDGLPAETVVPVTPDDGDIRRIDGMLNAAYRQAMLENTDQPWLDRGHWLGWPAALL
                     TLIWFRRGWTMRWAAAVLLAAAFAGPAPARADGVVDWFLTPDQQGRLAFQQNDFSRAG
                     ELFTDPLWRGYALYRDGQYETAVEVLDRVETAQAAFIQGMAHIKSRTYRDGVRAFETA
                     LERDPDYPNAEANLAVAREIVDYVERAREQSDTGEEAGIGADDVVIDNESGRGAETEM
                     EAPEEGGDGGLLTTDQWMSTVDTRTGDFLRQRFAIEAARRQAPAAADAGAGAPAAAPA
                     EGEGGTAE"
     gene            1374..2858
                     /locus_tag="P5558_16095"
     CDS             1374..2858
                     /locus_tag="P5558_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25891.1"
                     /translation="MRRAPHAAILLLLASSPLAQEADRPSAGDPLVPLELQTLAAPPA
                     RNTERPGDATTTGSATEATGEGGKEAATEATAEEPLSASDAPVLQVTFEETEAIPGQP
                     LSLRLTVLAPTFMPDPPVWPSLEAPNLLVRLPERSTGPTSEEVGGETWSGVTRHYRIS
                     PMVPGEFAIPPQEVIVTWADPETSEPRKVTLTTEPLTFSGVLPEGAEGLDPFIAAEAL
                     ELKQEVAGEPEAMQPGDSVTRTIKATIRGTSPMFLPALLPPTDVAGVAAYPDEPVLEE
                     SDDRGVLEGTRAESVTLVAEGGGSGEAPAVSLDWYNLKSGEVETASVDGFPIEVDGPP
                     ASSAEPRDWRAIGLASLAALLALAVVAWLLRRLLPPFGRWLRERRTERLASEGHAFGQ
                     LQRAVARRDEPALRPALDAWAAKVEGHDPREHPGVRHALVAIGAARYGRGASGDAGAG
                     WRALAAALPEARRDALARDRLAALPPLNPGASGGWSAAIVSDRE"
     gene            3001..4203
                     /locus_tag="P5558_16100"
     CDS             3001..4203
                     /locus_tag="P5558_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25892.1"
                     /translation="MTDADTGRSLAERFDRFDIAQGGPFAELQARFAILREKAATAPR
                     RAALYVAIAFIVPLLVTVLTGTAWGPLVTRPFLLDWGVWARFVVAIAILVLMERLVAE
                     RLKAHLRQFVETPLLAPGAMPAAGEALARALRRRDSAVAEIVCVVLAYAATLVGAYAG
                     ATTQGSSWLSTGAPGAEQLSVAGWYVALVSGPLFWFLLLRWLWRHLVWALLLYEISRL
                     ELRLVVTHPDGVGGLAFIGQYPNAFSAMVLAMSCVLAAAIAKAFQQDALELTAYGYMM
                     AAWLITVLLLFGVPLLAFRRPLGRLKQQALLASSAAATRHFRAAERAALGGNLAAATD
                     ADATAVGDIPNPTATYTAAKKLRTIAFSREALLPVATAAILPLVAAGSTRLPFAELWK
                     IAKKLLLL"
     gene            4215..5222
                     /locus_tag="P5558_16105"
     CDS             4215..5222
                     /locus_tag="P5558_16105"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRY25893.1"
                     /translation="MYRLALTFLFLAAGPAAADQLCEGYAGLPTGDDATAGMVAIPGG
                     TFIMGDADERPEERTPHEVTLSPFMIDRHEVTNAQFRAFVEATGYVTVAEAGLDPAEY
                     PGLPPELLVPGGMVFDEPETADLRGSVAQWWRYVPGASWRHPAGPESSIEGLDNHPVV
                     QVAHADALAYAQWLGRDLPTEAEWEFAARGGLEGATYTWGDSYDPVQGWLANSWQGSF
                     PVSDQALDGHHGTAPVGCFEANGYGLFDMGGNVWEYARDYYLPGHFPDPATDPAGPEA
                     SLSARYSPTRMPQVVVKGGSWLCAPNFCLRYRPAARQPQEPDLSSNHIGFRTVLRDRA
                     G"
     gene            5268..6578
                     /locus_tag="P5558_16110"
     CDS             5268..6578
                     /locus_tag="P5558_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25894.1"
                     /translation="MPEGTRTSIALTLLAVLSLAGCESFGRGVTQAVLEGTGEPAEDT
                     RACEVEGRPFVGIEPFLAAQDALPPFEEGDTDRPEVKVLYVHGIGTHAPGHGTALRQN
                     LATSLGLEIRAPRPKRIVIAHPEFPDQDLGEINVTRLTDASRRRDLLFYELTWSPITQ
                     PDKDLLAFDKEQDYVLRRAAVNQAMRTFVNDIAPDPLAYAGKKREPILMSVTQSICWM
                     VSRGWSDLPELTENTWCGPGLPGFGDRVTEDDFVIITHSLGSRATLDAMQALTQLPVT
                     ADPEIKRLADDFARRDIQVFMLSNQLPLLEAGREGQQVVGQVDAYCGPNATKPGRFFN
                     KTQIIAFSDPNDLMSYPVPDQFADRYIESRLCPSVTNVTINVAAVNSLLGLGDVANPL
                     GAHTGYGPDERVGALIARGAGNPNVAPVIAERCTWRETDEGLMR"
     gene            6590..8176
                     /locus_tag="P5558_16115"
     CDS             6590..8176
                     /locus_tag="P5558_16115"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HRY25895.1"
                     /translation="MPTIRSKRQDGWLRLCRGALLALLLAAPVDALAQEAETADPAAS
                     DTLVVEDEVRALVAPVALYPDPLLAVVLQAAMFPVDVVQADRFIQSYQDDQTLEPSAE
                     WDPAVTALLGYPSLLAEMNEHLDWTQAMGDAVYDQLEDVQEAIQDLRQGAYAMGVLAS
                     NEVQEVVVTEGIIEIRPTSPDRISIPQYDGFALLGSLETVGEAAAEEAADATVAADAA
                     PADEPAVADEAEVAEEAEAGVVEDAPPAETAAEPEVAEAAAIEEGEYAEETGYAEADP
                     YASSYAAPVDPTYAQPPVYAAAPPPVAYAPPASSAWGSTATFLGGAAIGGLLGYAIFD
                     DDDNDNDGGNNRRNDINIEDSTIVVGGDGDRLNLDRDQKNRVNAELRDRRDRNQQVAR
                     RDRDRNRQVAALPGRDSTGRQRERQRDISLPQRDRDRPAKVERKQQRDTKAVKAPKQR
                     KGAVADIKRPSQTKKDAKRGKQSRQISAARSGKSRPQAAQRGGGKKQALAPKKGGKKK
                     AKAKSNRGKKSRGGKKRGRG"
     gene            8188..9102
                     /locus_tag="P5558_16120"
     CDS             8188..9102
                     /locus_tag="P5558_16120"
                     /inference="COORDINATES: protein motif:HMM:NF022892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 family protein"
                     /protein_id="HRY25896.1"
                     /translation="MKEFRTIAFVAGLAGGFLLAGSASAGQPAFAAPEAALEAFRVAL
                     AAPDGSALLELLGDEHRDELIGADPASARQTMAAVRNAAAEAIALAPGDAPGTFDVLL
                     GRAGWPMPIPLVQTAEGWVFDTEAGLEEIIDRRIGENELAAIAAARAYVEAQLLYGSV
                     DRNGDGVPEFAQRLVSTPGEHDGLFWPTAAGEEPSPLAPLASSEADYLAYRESGEPYY
                     GYRFKVLTAQTGNALGGAYEYLINGRMLAGFGLLAWPADYGNSGIMSFIVNQLGDVYE
                     ADLGEGTDAVAEAITAFDPAADWALVED"
     gene            9224..10819
                     /locus_tag="P5558_16125"
     CDS             9224..10819
                     /locus_tag="P5558_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY25897.1"
                     /translation="MKHLTLSLLLGTALVLAAPGPASEAATPPDVFVQAMTIDDIITL
                     DPAEIFEFSGAEYGAQVYDRLITYPVDDVEDIRGHVAESWEVSEDGKTYVFKIRDGIT
                     FHSGNPLTAEDVAFSLQRVVKLDMSPAFILTQFGFTADNVEEKIRATGPMEVTIEVDQ
                     AYAPTFFLYSLTAGVGSVVDKETVLANEVDGDLGYNWLKVNSAGSGPFKLRAWKPNES
                     LTLDANPDYWLGAPGVTRAITRHIAEAATQQLLIEKGDIDVARNLGPDQVAALEANPD
                     IVIESVPKGAIYYLGLNQKNENLAKPEVREALKWLIPYQEMVDTLVKGDMVVHQSFLP
                     KGFLGVLEDQPYSLDVERAKELLAEAGLPDGFSVTMDVRSISPVTEMAQAIQAVWAEA
                     GIDLELIPGDGAQTLTKYRARNHDIYIGRWGPDYQDPHTNADTFARNPDNADDAQSKP
                     LAWRNAWDIPEMTAMADAAVLERDPAKRAEMYREIQRIHQQTSPFVIMFQDIETIAER
                     SKVEGTIWGPSFDDNRYWKITKN"
     gene            10857..>11105
                     /locus_tag="P5558_16130"
     CDS             10857..>11105
                     /locus_tag="P5558_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25898.1"
                     /translation="MLRPAGTLLRLLATLALTFLGLLVVTFLIGRVVPVDPVLAAVGA
                     RASPAVYARVRAEIALQHALQEGDLGQSVLTARPVLDDI"
BASE COUNT         1705 a         4033 c         3840 g         1527 t
ORIGIN      
        1 ctgctggcgc tgggcgccgc cgggcccacc tggtcgcgca tgccggaccc gttcgtggcg
       61 cagtcggcac ccgtcgtcgt cgtcctggag gtcacgcctt ccatggaggc gaaggacatc
      121 gccccgtcgc ggctggagcg cggcaagcag aagatccgcg acctcctcga gctgcgcgcc
      181 ggcgcccgca ccgccctcgt cgcctatgcc ggcagcgccc acggcgtcgt gccgatgacc
      241 gaggacccgg ccgtgatgac cccctacctc gcagggctcg cccccgacgt gatgccgaac
      301 gagggcgagc gggccggcga cgccctggcg ctcgccgtcg acctcctggc gcaggagtcg
      361 tcgccgggcg gcgtgctctt cgtcgccgac ggtgtggacc cggcagacgt ggcgacgctg
      421 accgggcccg atgcgccggc gctggcggtg ctggcgatgc tgcccgaggg cacgagcgat
      481 cgcgggctgg acgggctgcc ggcggaaacg gtggtccccg tcacccccga cgatggcgac
      541 atcaggcgga tcgacgggat gctcaatgcc gcctaccgcc aggccatgct ggagaacacg
      601 gaccagccct ggctggaccg cggccactgg ctcggctggc ccgcggccct gctcacgctg
      661 atctggttcc ggcgcggctg gacgatgcgc tgggccgccg ccgtcctgct cgcggccgcc
      721 ttcgccggac cggcgccggc gcgggccgac ggcgtcgtcg actggttctt gaccccggac
      781 cagcagggcc ggctcgcctt ccagcagaac gacttcagcc gcgctggcga gctcttcacc
      841 gatccgctct ggcgcggcta cgcgctctat cgcgacggcc agtacgagac ggcggtcgag
      901 gtcctggacc gggtcgagac ggcccaggcg gccttcatcc agggcatggc gcacatcaag
      961 tcgcggacct accgcgacgg tgtgcgcgcc ttcgagacag cgcttgagcg cgatcccgac
     1021 tacccgaacg ccgaggcgaa cctcgcggtc gcccgggaga tcgtcgatta tgtcgagcgc
     1081 gcgcgcgagc agtcggacac cggcgaggaa gccggcatcg gcgccgacga cgtggtcatc
     1141 gacaacgaat ccgggcgcgg cgccgagacc gagatggaag cgccggagga aggcggcgac
     1201 gggggcctgc tcacgaccga ccagtggatg agcacggtcg acacccgcac cggcgacttc
     1261 ctccgccagc gtttcgcgat cgaggcggcc cggcgccagg cgccggcggc tgccgacgcg
     1321 ggcgccgggg cgccggcggc ggcacccgcc gagggcgaag gaggcaccgc cgaatgaggc
     1381 gcgccccgca cgccgcgatc ctgctcttgc tggcatcgtc gccgctggcg caagaagccg
     1441 atcgaccatc cgccggcgac ccgctcgtgc cgctcgagct gcagaccttg gcggcgccac
     1501 ctgcccgtaa caccgagcgc ccgggcgatg ccacgacgac cgggagcgct accgaggcca
     1561 cgggtgaggg cggcaaggag gctgcgacgg aggcgacggc cgaggagccg ctgtcggcga
     1621 gcgacgcgcc cgtgctgcag gtcaccttcg aggagaccga ggccattccg ggccagccgc
     1681 tcagcctgcg cctgaccgtg ctcgccccca ccttcatgcc cgatcccccc gtctggccga
     1741 gcctcgaggc gccaaacctg ctggtccgcc tgcccgagcg atcgaccggg ccgacgagcg
     1801 aggaagtggg cggcgagacc tggtccggcg tcacgcggca ctaccgcatc tcgcccatgg
     1861 tgccggggga gttcgcgatc ccgccgcagg aggtcatcgt cacctgggcc gatccggaga
     1921 cgagcgagcc ccggaaggtg acactgacga ccgagcccct cacgttctcg ggcgtgctgc
     1981 cggaaggtgc ggagggcctc gacccgttca tcgccgccga ggcgctcgag ctgaagcagg
     2041 aggtggcagg cgagcccgag gcgatgcagc cgggcgacag cgtcacgcgg acgatcaagg
     2101 ccaccatccg cggcacctcg cccatgttcc tcccggcact cctgccgcct acggatgtgg
     2161 cgggcgtggc tgcctatccc gacgagccgg tgctcgagga gtccgatgat cgtggcgtcc
     2221 tcgagggcac ccgggcggaa agcgtgacgc tggtggcgga aggcggcggc agcggcgagg
     2281 cgccggcggt ctcgctcgac tggtacaacc tgaaatccgg cgaggtggag acggcatccg
     2341 tcgacggctt cccgatcgag gtcgacggcc cgcccgcgtc gagcgcggag ccgcgcgact
     2401 ggcgcgccat cgggctcgcc agcctggccg ccctgctggc actggcagtc gtcgcctggc
     2461 tcctgcgtcg gctgctgccg cccttcggcc gctggctgcg cgagcgccgt accgaacgtc
     2521 tggcctcgga gggacatgcc ttcgggcagc tccagcgagc ggtggcgagg cgtgacgagc
     2581 cggccttgcg ccctgcgctc gatgcgtggg cggcgaaggt ggaggggcac gacccgcgcg
     2641 agcacccggg cgttcgtcat gcgctggtgg cgatcggtgc cgcccgctac ggccgcgggg
     2701 cctcgggcga cgccggggcc ggctggcgcg cgctcgccgc cgccctgccg gaggcgcgcc
     2761 gcgacgccct tgcgcgcgac aggctcgccg ccctgccgcc cttgaaccca ggtgcctcgg
     2821 gcgggtggtc ggcggcgatt gtctcggatc gcgaatgatg ctgtcattgg aaacacacga
     2881 ggaaggcgct gtcggcaacg accggcggcc ggcgcacaag tgcggggagg gcgatacggt
     2941 gacgcattgg cgatcggcgg gctggccatc gctcgggccg cgccacggcg cgggacgctc
     3001 gtgaccgacg ccgacaccgg acggtcattg gccgagcggt tcgaccgctt cgacatcgcc
     3061 cagggcgggc cgttcgcgga gctgcaggcg cgattcgcta tcctgcggga gaaggcggcc
     3121 acggcgccgc ggcgggcagc gctgtacgtc gccatcgcgt tcatcgtccc cctcctcgtc
     3181 actgtcctca ccggcacggc ctggggcccg ctcgtcacgc ggcccttcct gctcgactgg
     3241 ggtgtgtggg cacggttcgt ggtggcgatc gcgatcctcg tcctgatgga gcggctggtc
     3301 gccgagcgcc tgaaggccca tctgcggcag ttcgtcgaaa cgcctctgct ggcacccggc
     3361 gccatgccgg ccgcgggcga ggcgctcgcg cgtgcgctgc ggcgtcgcga ctccgctgtc
     3421 gccgagatcg tctgcgtcgt gctcgcctac gcggcgacgc tcgtcggtgc ctatgcggga
     3481 gccaccaccc aggggagctc ctggctctcc accggcgcac ccggtgccga gcagctcagc
     3541 gttgccggct ggtatgtcgc cctggtcagc ggcccgctct tctggttcct gctgctccgg
     3601 tggctttggc gccatctcgt ctgggcgctc ctgctctacg agatctcgag gctggagctg
     3661 cggctcgtcg tcacccatcc ggacggggtc ggcggcctcg cgttcatcgg tcagtacccc
     3721 aatgcctttt ccgccatggt gctggcgatg agctgcgtgc ttgccgcggc gatcgccaag
     3781 gcgttccagc aggatgctct cgagctgacc gcctacggct acatgatggc cgcctggctg
     3841 atcaccgtgc tcctgctgtt cggcgtgccg ctgctcgcct tcaggcggcc gctcggccgg
     3901 ctcaagcagc aggcgctcct ggcttcctcc gcggcggcga cccgccactt ccgcgccgcc
     3961 gagcgcgcgg cgctgggtgg caacctcgcg gccgccaccg acgccgacgc cacggccgtc
     4021 ggcgacatcc cgaacccgac cgccacctac accgcggcga agaagctgcg caccatcgcc
     4081 ttcagccgcg aggcgctcct gccggttgcc acggccgcca tcctgccgct cgtggcagcg
     4141 ggctcgaccc ggctgccgtt cgccgagctc tggaagatcg ccaagaagct gctcctcctt
     4201 tgacaaggga cctcatgtat cgacttgcgc tcacctttct cttcctggcg gccgggcccg
     4261 ccgccgcgga ccagctctgc gaaggttatg ctgggctgcc gaccggcgac gatgcgaccg
     4321 ccggcatggt cgccatcccg ggcggcacct tcatcatggg cgacgccgac gagcggccgg
     4381 aggagcgcac gccgcacgag gtgacgctct cgccgttcat gatcgatcgc cacgaggtga
     4441 ccaacgccca gttccgcgcc ttcgtcgagg ccacgggcta tgtcaccgtg gccgaggcgg
     4501 gactcgaccc ggcagagtat ccggggctcc cgcccgagct gctggtgccg ggcggcatgg
     4561 tcttcgacga gcccgagacc gccgacctgc gcggcagcgt cgcccagtgg tggcgctacg
     4621 tgccgggcgc gagctggcgt cacccggccg gccccgagag ctcgatcgag ggcctcgaca
     4681 accaccccgt cgtgcaggtg gcccacgccg acgcgctggc ctacgcccaa tggctggggc
     4741 gcgacctgcc gaccgaggcg gaatgggaat tcgcggcgcg cggcggcctg gagggcgcca
     4801 cctacacctg gggcgacagc tacgatcccg tccagggctg gctcgccaac tcctggcagg
     4861 gaagttttcc ggtgagcgac caggcgctcg acggccatca cggcacggcc ccggtcggct
     4921 gcttcgaggc caatggctac ggtctcttcg acatgggcgg caacgtctgg gaatacgcca
     4981 gggactacta cctgccgggc cattttcccg acccggccac agacccggcc ggtcccgagg
     5041 cgtcgctctc cgcccgctac agcccgaccc gcatgccgca ggtcgtggtc aagggcggct
     5101 cctggctctg cgcccccaat ttctgcctcc gctaccgccc ggcggcgcgg cagccgcagg
     5161 agccggacct cagctccaac catatcggct tccgcaccgt gctgcgcgat cgggcaggct
     5221 gagcgaacgt gccggaccat ccagcgacga ggtcgcaagg agacgaaatg cctgaaggca
     5281 ccagaaccag catcgccctg accctgctcg ccgtgctctc gctcgctggc tgcgagagct
     5341 tcggccgggg cgtcacgcag gccgtgctgg aaggcaccgg cgagccggcc gaggacacac
     5401 gggcctgcga ggtggaaggt cggccgttcg tcggcatcga gcccttcctc gcggcccagg
     5461 acgccctgcc gccgttcgag gagggcgaca cggaccggcc cgaggtcaag gtgctctacg
     5521 tgcacggcat cgggacccac gcacccgggc acggcacggc cctgcgccag aacctcgcca
     5581 cctcgctcgg gctggagatc cgggcccccc ggccgaagcg catcgtcatc gcccatccgg
     5641 agttccccga ccaggacctg ggcgagatca acgtgacccg gctcaccgac gccagccgtc
     5701 ggcgcgacct cctgttctac gagctgacct ggtcgccgat cacgcagccc gacaaggacc
     5761 tgctcgcctt cgacaaggag caggactacg tgctgcgccg ggccgccgtg aaccaggcga
     5821 tgcgcacctt cgtcaacgat atcgcccccg acccgctggc ctatgccggc aagaagcgcg
     5881 agccgatcct gatgtcggtc acccagtcga tctgctggat ggtgtcgcgc ggctggagcg
     5941 acctgcccga gctcaccgag aacacctggt gcggccccgg cctccccggc ttcggcgacc
     6001 gggtgaccga ggacgacttc gtgatcatca cgcacagcct gggcagccgg gcgaccctcg
     6061 acgccatgca ggcgctgacc cagctcccgg tcacggcgga cccggagatc aagcggctcg
     6121 ccgacgattt cgcccgtcgt gatatccagg tcttcatgct gtcgaaccag ttgccgctgc
     6181 tcgaggccgg ccgcgagggc cagcaggtgg tcgggcaggt ggatgcctat tgcgggccga
     6241 acgcgacgaa gccggggcga ttcttcaaca agacccagat catcgccttc tccgacccca
     6301 atgacctgat gagctacccg gtgccggacc agttcgccga caggtacatc gagtcgcgcc
     6361 tctgcccgag cgtcaccaac gtcacgatca atgtggccgc ggtgaactcc ctcctgggcc
     6421 tgggcgatgt cgccaacccg ctcggcgccc acaccggcta cgggccggac gagcgggtcg
     6481 gtgcgttgat cgcgcgcggc gccggcaacc cgaacgtggc gccggtcatt gccgagcgct
     6541 gcacgtggcg cgagaccgac gagggcctga tgcggtgaga gaggtgagca tgcctacaat
     6601 ccgatccaag cgccaggatg gctggctcag gctgtgccgg ggcgcgctcc tggcgctgct
     6661 tctcgcggca cccgtcgatg cgctggcaca ggaggccgag accgccgatc ccgcggcatc
     6721 ggacacgctg gtcgtcgagg acgaggtgag ggcgctcgtc gcccccgtcg ccctctaccc
     6781 cgacccgctc ctcgccgtcg tgctgcaggc ggcgatgttc ccggtcgacg tcgtccaggc
     6841 cgaccggttc atccagagct accaggacga ccagacgctc gagccctcgg cggaatggga
     6901 cccggcggtc acggcgctcc tgggctaccc gtcgctgctc gccgagatga acgagcatct
     6961 cgactggacc caggcgatgg gcgacgccgt ctacgaccag ctcgaggacg tccaggaggc
     7021 gatccaggac ctgcgccagg gcgcctacgc catgggcgtc ctcgcctcca acgaggtgca
     7081 ggaggtcgtc gtcaccgagg ggatcatcga gatccggccg acctcgccgg accggatctc
     7141 gatcccccag tatgacggct tcgcgctcct cggctcgctc gagacggtgg gcgaggcggc
     7201 cgcggaggag gccgccgacg ccacggtggc cgccgacgcg gcaccggccg acgagccggc
     7261 cgtcgcggac gaggcggagg tggcggagga ggccgaggcc ggggtcgtcg aggacgcgcc
     7321 cccggcagag acggctgccg agcctgaggt cgccgaagcg gcggcgatcg aggaaggcga
     7381 gtatgccgag gaaaccggct acgccgaggc cgacccctat gcgtcgagct acgccgcacc
     7441 ggtcgatccg acctacgcgc agccgcccgt ctatgccgcg gcaccgccgc ccgtcgccta
     7501 tgcgccgccg gcgagctccg cctggggctc gacggcgacc tttctcggcg gggccgcgat
     7561 cggcggcctg ctcggctatg ccatcttcga cgatgacgac aacgacaacg acggcggcaa
     7621 caaccggcgc aacgacatca acatcgagga cagcacgatc gtcgtcggcg gcgacggcga
     7681 tcgcctgaac ctcgatcgcg atcagaaaaa tcgcgtcaac gcggagctgc gcgaccgtcg
     7741 cgatcgcaac cagcaggtgg cgcgacgcga ccgcgaccgc aatcgtcagg tcgcggccct
     7801 gcccgggcgc gattcgaccg gccggcagcg cgagcggcag cgcgacatca gcctgccgca
     7861 gcgcgaccgg gaccgcccgg ccaaggtcga gcgcaagcag cagcgagaca cgaaggcggt
     7921 caaggcgccg aagcagagaa agggcgccgt cgccgacatc aagcggccga gccagaccaa
     7981 gaaggacgcg aagcgcggca agcagagccg ccaaatatcg gcagccaggt cgggcaagtc
     8041 ccgaccgcag gcagcgcagc gcggcggcgg caagaagcag gcgctggcgc ccaagaaggg
     8101 tggcaagaag aaagcgaagg ccaaatcgaa ccggggcaag aagagccgcg gcggcaagaa
     8161 gcgcggcagg ggctgaggag cagatccatg aaagagtttc gcaccattgc gttcgtcgcc
     8221 gggcttgcgg gcggttttct cctggccggc tcggcctcgg ccgggcagcc ggctttcgcc
     8281 gcgcccgagg cagcgctgga ggccttccgc gtggcgctcg cggcgcccga cggcagcgcg
     8341 ctgctcgagc tcctcggtga cgagcaccgg gacgagctga tcggtgccga tccggcaagc
     8401 gccaggcaaa ccatggccgc cgtgcgcaac gccgccgccg aagcgatcgc gctggctccc
     8461 ggcgacgcgc ccggcacctt cgacgtgctg ctcggccgcg ccggctggcc gatgccgatc
     8521 ccgctggtcc agacggccga gggctgggtc ttcgacaccg aggccgggct cgaggagatc
     8581 atcgaccgac ggatcggcga gaacgagctg gccgcgatcg ccgcggcccg ggcctatgtc
     8641 gaggcacagc ttctctacgg ctcggtcgac cgcaacggcg acggcgtgcc cgagttcgcc
     8701 cagcgcctcg tcagcacgcc gggcgagcat gatggcctct tctggccgac agcggccggc
     8761 gaggagccga gcccgctggc cccgctcgcc agcagcgagg ccgactacct cgcatatcgc
     8821 gagagcggcg agccctatta cggctaccgg ttcaaggttc tgacggcgca gaccgggaat
     8881 gccctgggtg gcgcctacga atacctgatc aacggccgga tgctcgccgg cttcggcctg
     8941 ctcgcctggc ccgccgacta cggcaattcg ggcatcatga gcttcatcgt caaccagctc
     9001 ggcgacgtct acgaggccga cctcggcgag ggcaccgacg ccgtcgccga ggcgatcacg
     9061 gcattcgatc ccgccgccga ttgggccctg gtcgaggact gacaacttcg gcccggcgct
     9121 cgccgacatt gccttcgaac gcggattctt ccaccttcgg ggcgagcgag agcgccggtc
     9181 gacccgaagc gcaagaacgc cccggtcaat ggaggtacat ttcatgaagc atctgacgct
     9241 gtccctgctg ctcggcaccg ccctcgtcct ggccgcgccc ggcccggcga gcgaggcggc
     9301 cacgccgccg gacgtgttcg tccaggcgat gaccatcgac gacatcatca ccctcgaccc
     9361 ggccgagatc ttcgagttct ccggggccga gtacggtgcc caggtctacg accggctgat
     9421 cacctatccg gtcgacgacg tcgaggacat caggggccac gtcgccgaga gctgggaggt
     9481 ctccgaggat ggcaagacct acgtcttcaa gatccgggac ggcatcacct tccattccgg
     9541 caacccgctg accgccgagg acgtcgcctt ctcgctgcag cgcgtggtca agctcgacat
     9601 gtcgccggcc ttcatcctga cccagttcgg cttcaccgcc gacaatgtcg aggagaagat
     9661 ccgtgccacc gggccgatgg aggtcaccat cgaggtcgac caggcttacg ccccgacctt
     9721 cttcctctat agcctcaccg ctggcgtcgg ctcggtcgtc gacaaggaga cggtgctggc
     9781 caacgaggtg gacggcgatc tcggctacaa ctggctgaag gtcaactcgg ccggctccgg
     9841 ccccttcaag ctgcgcgcct ggaagccgaa cgagagcctg accctggatg ccaaccccga
     9901 ctactggctc ggcgcacccg gcgtcacccg cgcgatcacc cgccatatcg ccgaggccgc
     9961 gacccagcaa ttgctgatcg agaagggcga catcgacgtc gcccgcaatc tcggcccgga
    10021 ccaggtggcg gccctcgagg ccaaccccga catcgtcatc gagtcggtgc cgaaaggtgc
    10081 gatctactat ctcggcctga accagaagaa cgagaacctc gccaagcccg aggtgcgcga
    10141 agcgctcaag tggctgatcc cctaccagga gatggtcgac accctggtca agggcgacat
    10201 ggtggtgcac cagtccttcc tgcccaaggg cttcctcggc gtcctcgagg accagcccta
    10261 ctcgctcgac gtcgagcggg cgaaggaatt gctcgccgag gccgggctgc cggacggctt
    10321 ctcggtcacg atggacgtgc gcagcatctc gcccgtcacc gagatggcgc aggccatcca
    10381 ggcggtctgg gccgaggccg gcatcgacct cgagctgatc ccgggcgacg gggcgcagac
    10441 cctgaccaag taccgggcgc gcaaccacga catctatatc ggccgctggg gcccggacta
    10501 tcaggacccg cacaccaacg ccgacacctt cgcgaggaat cccgacaacg ccgacgacgc
    10561 ccagtccaag ccgctcgcct ggcgcaatgc ctgggacatc ccggagatga ccgccatggc
    10621 cgacgccgcg gtgctcgagc gcgacccggc gaagcgcgcc gagatgtacc gcgagatcca
    10681 gcgcattcat cagcagacgt cgcccttcgt catcatgttc caggacatcg agacgatcgc
    10741 cgagcgctcg aaggtcgagg gcaccatctg gggcccgagc ttcgacgaca atcgctactg
    10801 gaagatcacc aagaactgag cgtggcgcag gcgctccggg tgccgggcgg cccgccatgc
    10861 tgaggccggc cggcaccctg ctcaggctgc tggcgaccct ggccctcacg ttcctcggcc
    10921 tgctcgtcgt caccttcctg atcggccggg tcgtgccggt cgatcccgtg ctggccgccg
    10981 tcggcgcccg cgcctcgccc gccgtctacg cccgggtgcg ggccgagatc gccctccagc
    11041 acgcgctgca ggagggcgat ctcggccaga gcgtgctgac cgcccggccg gtgctggacg
    11101 acatc
//
LOCUS       DAOTFI010000167        11062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7018_length_11062_cov_6.607886, whole genome shotgun sequence.
ACCESSION   DAOTFI010000167 DAOTFI010000000
VERSION     DAOTFI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11062
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7018_length_11062_cov_6.607886"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="P5558_16135"
     CDS             <1..575
                     /locus_tag="P5558_16135"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HRY25899.1"
                     /translation="ARRMGAVVTATDVRPAAKQEIESLGAKFVGFIPEDGATSGGYAR
                     ALTPEEQKKQAEMVAEHLKSQDIVVTTAQIPGRKAPRIITAAMVDSMKAGSVVMDLAA
                     ETGGNVEGSVAGETVTTANGVTLIGAMNLPSRLAPASSILYAKNLMNFVMLLVDKEAK
                     AFTVDEEDDLVKGALITKGGSIVHPNLQNA"
     gene            621..878
                     /locus_tag="P5558_16140"
                     /pseudo
     CDS             621..878
                     /locus_tag="P5558_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
     gene            898..2352
                     /locus_tag="P5558_16145"
     CDS             898..2352
                     /locus_tag="P5558_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRY25900.1"
                     /translation="MSMNLAAIGYLVAAVCFILALRGLSHPATSQAGLRYGMGGMALA
                     IVVSLMVLPHPSFLAYLLIVVGLGAGGAVGWVIAKKIEMTAMPQLIAGFHSLVGLAAV
                     LVAAAAFLNPVDFGLVKADGSIKMLSLFEMGLGTIIGAITFTGSIIAFGKLQAKLPPM
                     IESRLGPLKGPIATAMSSKPITLPHRHLLNVAIAVVTLLLLAAFVSGESGFAFFLMTL
                     LAFALGVLLIIPIGGADMPVVISMLNSYSGWAAAGIGFTLQNPMLIVTGALVGSSGAI
                     LSYIMCKAMNRSFVSVILGGFGETGAVAADDGDKTVKAGSADDASFIMENAQSVIIVP
                     GYGMAVAQAQHAVRELYDVLTKKGVEVRFAIHPVAGRMPGHMNVLLAEANIPYDVVFE
                     MEDINSDFQQADVAYVIGANDITNPAAKNDPQSPIFGMPVLDVDKAKAVFFVKRSMAA
                     GYAGIENTLFYTPNTMMLFGDAKKMTEEIVKAIE"
     gene            2644..4383
                     /locus_tag="P5558_16150"
     CDS             2644..4383
                     /locus_tag="P5558_16150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HRY25901.1"
                     /translation="MAPSPELQEKINKALKKMRQAAPDGMAHMAALRQLPRVGFNDGL
                     IVPGDLLPVGTPPAVARNMALERAPLRGTVNVVVVLAEFSDRQFGANATVAHFEELFF
                     STGQLATGSVREYYTEATNGLVTIAGQVVGPYTLPNTLANYANGASGTGNTNPNSRDM
                     ARDAALAANADVNFSVYDNDGDGFVDAYVVVHAGMGAEQTGSGGDIWSHKWVMRSQLN
                     ADGTQLFAYLTIPENARIGVCAHELGHLLFGFPDLYDTDSSSEGVGNWCLMGGGSWNG
                     GGQTPAHPSAWCKANQGWATVTNVTSNGVRSIQAVETGHEIFRLWKDGGASQEYFLVE
                     NRQKTGFDSGLPGEGLCIWHIDEAISTNSNEAHPKVALEQADGKDDLGTAANRGDAGD
                     VYPGSAGKTIFDNGSSPGSRSYAGNDTCVAVASISASGPTMTADLRVRCGKSVVKDVS
                     DKGRDKRLEKRPDKIGEKRPDKLWDKSVVEDKRPDKPEIDKRSGYDKGWDYDYRDRFG
                     RAPERHGDLERRVEALESAFGGLAPFIDSSLRPDLEGSGLSAEEDLARMRDEMEQQTQ
                     SDKRLLDSPTHRR"
     gene            4431..5279
                     /locus_tag="P5558_16155"
     CDS             4431..5279
                     /locus_tag="P5558_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25902.1"
                     /translation="MRRSIVVLADPDDRGALLVARSLRLRGAPVALVATDQLLLAPVW
                     EHDPLGASRVVLGNGLELTDSSIGVLFCRARGVDPPQFRGAVESDRRYAQEEFFALLM
                     SWLARLGERVLNRPHPGNLHGTQCSLVEDLLSSAWSRGSPHGFAAASHARHLPPGAGP
                     VREYRPWGGRFDAGQPAGSGVPGSVVAGGPGMRLPPLPDGRHWNVTVIGDRLLGGQPP
                     AAARSLAFRLVRDRGLSFAAIELFQLADGGLGHGMIDPFPALDHAGEIEAAADHLALA
                     LSRCVA"
     gene            5276..6199
                     /locus_tag="P5558_16160"
     CDS             5276..6199
                     /locus_tag="P5558_16160"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25903.1"
                     /translation="MIVLCGIPSEPPMRMVEDAAARLGVETHHLNQRDAATTALSIDA
                     TGNGLAFRQHGPDGSRDLAAASGMFVRLTDWRVLPEYRRAGGTAMQDRLRIEAWHGLL
                     GDWLETTPMLVMNRTKPSNSNMSKPYQAQVIAAAGFRVPATIVTNQVAVVRAFKARHR
                     RVIFKSISAHRSIVTELTEEAERHLERIRLLPTQFQELIEGDDVRVHVVGAKLLATRI
                     RSRAIDYRYAGGEQGGAIFEPMTLPEVVAERCLGLSRRLGLPLCGIDLKLTVDGEYAC
                     FEVNPAPAFSSFEERSGQPIAEAIARALAGI"
     gene            6293..6586
                     /locus_tag="P5558_16165"
     CDS             6293..6586
                     /locus_tag="P5558_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25904.1"
                     /translation="MVSIVENWAVIIGTVIGIVQAGVAPGTARLEVRIERIEPVESYP
                     MLVTQKPGDRISISVPNGKVGEGLLNAPIEVRVRRGRDPDLVFAAPDWNPAPR"
     gene            complement(6609..6905)
                     /locus_tag="P5558_16170"
     CDS             complement(6609..6905)
                     /locus_tag="P5558_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25905.1"
                     /translation="MSLSTVGTVESNQAPRRALLRRLPLLPAEMLPGVDRLAIRQDAH
                     PARAIRLLPHLVAAARLHAEDRRRIGDGMGRCRLEQQNHGDEQPSGEMHGKVPH"
     gene            complement(6902..7366)
                     /locus_tag="P5558_16175"
     CDS             complement(6902..7366)
                     /locus_tag="P5558_16175"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRY25906.1"
                     /translation="MTALDLADPLVATYVIAATLIILKAICMPWLTVVRMMQEKGGFR
                     SPEDLRKTSLNPAPNPGQLEPNDRVERIRRIQQNDLESLPYFLVAGLIFVLTGPPLLL
                     AQILLYGYVVSRLLHFASYLTAQTHDMRAMLWTPGSLILFFITLWSLAAAVL"
     gene            7661..8848
                     /locus_tag="P5558_16180"
     CDS             7661..8848
                     /locus_tag="P5558_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY25907.1"
                     /translation="MKIRTIETLALKVPIGAKTFYSSQARFPERNSLLVKITTDDGLV
                     GWGEGGQYGPGEPVASAVVDVLGPRLIGRRADQPVRIWEDNYSFSRDFGRTGTYVEAM
                     SAIDIALWDIWGKSLGRPVHHLMGGAFRDRAMAYGTGCYYPEFFRDTPRMMEALAEEV
                     AGYEAAGVTAVKIKLGLLSVAEDRARIELIRNTVGPAVRLMADANHAYNAATALRIGR
                     VMDSHDFLWFEEPVPPEDHDGYRLLRTKLDTPIAGGECEFARFGFRDFIGKGCVDIVQ
                     PDICASGGFSEFAKILATAGQHGVLTIPHVWGSGVAVAAALQAIATIPPMPHTAAPVT
                     LQNEPMVEYDRTMNPLRDGILHENFKLEDGAVAIPDGPGLGISVDEGALAGYVAQRRV
                     VEG"
     gene            8862..9782
                     /locus_tag="P5558_16185"
     CDS             8862..9782
                     /locus_tag="P5558_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25908.1"
                     /translation="MELRIAMPPEPLPPPTAPRVARLPKNPIIRPHMDGRMGDNINGP
                     SLIRVPDWVEGALGRYYLYFGHHDGAYIRLAFADDLAGPWRMHEPGVLPLAASGFAGH
                     LASPDVHVDDEERRFRLFFHGSDTATGRGGEQRTRLALSRDGLRFEAREETLGVPYWR
                     VFRHGGWHYALGMPGRLYRSGDGVSGFEAGPVVFAPGMRHSAVAVDGDTAFVYYTRIG
                     DRPERILLATMDLRPPWTDWRPSEAVTVLAPEEAWEGADLPLEPSREGIVRGPVRQLR
                     DPAIFRDGGRTWLLYSVAGESGISMAEISG"
     gene            9866..10264
                     /locus_tag="P5558_16190"
     CDS             9866..10264
                     /locus_tag="P5558_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HRY25909.1"
                     /translation="MRRFSSFLLAGLALAGCTSVGQVGLMTRPGADVGAIVREARPYK
                     ELGAVEGEACRNFLLGIIPWGDSSASTAMANALDKAGGDALINVSVETTLYGFIPIYN
                     VFANTCTTVRGVAIKFDEGEQPGASASAAP"
     gene            complement(10283..10966)
                     /locus_tag="P5558_16195"
     CDS             complement(10283..10966)
                     /locus_tag="P5558_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY25910.1"
                     /translation="MTPAAAHRVVTTVGWLSRQPVSFQTQLLASSVLRTVAAGDLIHA
                     LDDPPGGLLGVASGHVDVLIAPGPFAPMLIHVGRPGWWVGVSSAVSGRRRCTELSART
                     DARILYVPTSALEAMAADDPEIWRRAAGITIRHLDEALSHIACLASSDARLRLIVTLA
                     RLADPPEIGSSRVELPISQGELGEMAALSRNSVGRLLSALAKDGCVEKRYGRLVIDPA
                     RLQVLIGDW"
BASE COUNT         1762 a         3590 c         3916 g         1794 t
ORIGIN      
        1 ctgcccggcg catgggggcc gtggtgaccg ccaccgacgt ccgcccggcg gcgaagcagg
       61 agatcgagag cctgggggcg aagttcgtgg gcttcatccc ggaggacggt gccacctccg
      121 gcggctacgc ccgggcgctg acgccggagg agcagaagaa gcaggccgag atggtggccg
      181 agcatctgaa gagccaggac atcgtggtga ccacggccca gatcccgggc cgcaaggcgc
      241 cccggatcat caccgccgcc atggtggact cgatgaaggc ggggtcggtc gtcatggacc
      301 tcgccgccga gacgggcggc aatgtcgagg gcagcgtcgc cggcgagacg gtgacgaccg
      361 ctaacggcgt gacgctgatc ggggccatga acctgccctc caggctcgcc ccggcctcgt
      421 cgatcctcta cgccaagaac ctgatgaact tcgtcatgct gctggtcgac aaggaagcga
      481 aggcgttcac ggtcgacgag gaggacgacc tcgtcaaggg tgccctgatc accaaaggcg
      541 ggtcgatcgt gcaccccaac ctgcagaacg cctgagcggg agagcgcgcc atggaagcca
      601 tcgatcccgg ggtcttcaac ctcatcatct tcgtgctcgc gatcttcgtg ggctactacg
      661 tcgtctggtc ggtgaccccg gcgctgcaca cgccgctcat gtcggtgacc aatgcgatca
      721 gctcggtgat catcgtcggc gccctcatcg cggtgggcgc cgcctcgggc ttcgccacgc
      781 tgctcggctt catcgccgtg ctgctggccg ccgtgaacat cttcggcggc ttcgcggtga
      841 cccagcgcat gctcgccatg ttcaagaaac gcacgtgagg cggcagggga aacgcgcatg
      901 agcatgaatc tcgccgccat cggctatctg gtcgccgccg tctgcttcat cctggcgctg
      961 cgcggcctct cgcatcccgc gacgtcgcag gccggcctgc gctacggcat gggcggcatg
     1021 gcgctcgcca tcgtcgtctc gctcatggtg ctgccccacc cctccttcct ggcctatctg
     1081 ctgatcgtcg tcgggctcgg cgccggcggt gcggtcggct gggtgatcgc caagaagatc
     1141 gagatgacgg cgatgccgca gctcatcgcc ggcttccact cgctggtcgg cctcgccgcc
     1201 gtgctggtgg cggcggcggc cttcctcaac ccggtcgatt tcggcctggt caaggcggac
     1261 ggctcgatca agatgctgtc gctcttcgag atggggctcg gcaccatcat cggggcgatc
     1321 accttcaccg gctcgatcat cgccttcggc aagctgcagg cgaagctgcc gccgatgatc
     1381 gagagccggc tcgggcccct gaaggggcca atcgcgacgg cgatgagcag caagccgatc
     1441 accttgccgc atcggcatct gctgaatgtg gcgatcgcgg tcgtcaccct gctgctgctc
     1501 gctgccttcg tcagcggcga gagcggcttc gccttcttcc tgatgacgct cctggccttc
     1561 gccctcggcg tgctcctgat catcccgatc ggcggcgccg acatgccggt ggtcatctcg
     1621 atgctcaact cgtactcggg ctgggccgcg gccggcatcg gcttcaccct gcagaacccg
     1681 atgctgatcg tcaccggcgc cctcgtcggc agctccggtg cgatcctctc ctacatcatg
     1741 tgcaaggcga tgaaccgctc gttcgtctcg gtcatcctcg gcggcttcgg cgagaccggg
     1801 gcggtggcgg cggatgacgg cgacaagacg gtcaaggcgg ggtcggccga cgatgcgtcc
     1861 ttcatcatgg agaacgcgca gtcggtgatc atcgtgccgg gctacggcat ggcggtggcc
     1921 caggcgcagc acgcggtgcg cgagctctac gacgtgctga ccaagaaagg cgtggaggtg
     1981 cgcttcgcca tccatccggt ggccgggcgc atgcccggcc acatgaacgt gctcctggcc
     2041 gaggccaaca tcccctacga cgtcgtcttc gagatggagg acatcaacag cgacttccag
     2101 caggcggacg tggcctatgt catcggcgcc aacgacatca ccaacccggc cgccaagaac
     2161 gacccgcaga gcccgatctt cggcatgccg gtgctggacg tcgacaaggc caaggccgtc
     2221 ttcttcgtca agcgctcgat ggcggcgggc tatgccggca tcgagaacac gctcttctac
     2281 acgcccaaca ccatgatgct gttcggcgat gccaaaaaga tgacggagga gatcgtcaag
     2341 gcgatcgagt agaccggcgc atgcacgacc ggcccgaagc ctcccgccat ggatatcgat
     2401 gatggctgtc gaccgttgcc gacggatttg gtataaagga ccggctaacg acaagcctct
     2461 tactgcctgg taaatcagcc gcaacaggct ggaggtggtt gaagctcttg tttcgaccgt
     2521 aggggggatc agatgtccag tcacggcgaa gacaacgacg ggtccgacgc gctcgagctt
     2581 ctatcgagta ttcgccacat ttatgcagaa aaccgggcga cggacgacgg cgagcgctgc
     2641 tgcgtggcac catcgccgga gctgcaggag aagatcaaca aggcgctgaa gaagatgcgc
     2701 caggcggcgc ccgacgggat ggcgcacatg gcggcgctgc gccagctgcc gcgggtcggc
     2761 ttcaatgacg ggctgatcgt gccgggcgat ctcctgccgg tcggcacgcc accggccgtg
     2821 gcccgcaaca tggcgctgga gcgggcaccg ctgcgcggca cggtcaatgt cgtcgtcgtg
     2881 ctggcggagt tctccgaccg ccagttcggc gcgaatgcga ccgtggcgca tttcgaggag
     2941 ctgttcttct cgaccgggca gttggcgacc ggcagcgttc gcgaatacta caccgaggcg
     3001 accaacgggc tggtgacgat cgcggggcag gtcgtcggcc cctataccct gccgaacacg
     3061 ctggccaatt acgcgaacgg cgcatcgggg acgggcaaca ccaacccgaa ttcccgcgac
     3121 atggcccgcg atgcagcgct cgctgccaat gcggatgtga acttcagcgt ctacgacaac
     3181 gacggcgacg gcttcgtcga cgcctatgtg gtcgtgcatg cgggcatggg cgccgagcag
     3241 accggcagtg gcggcgatat ctggtcgcac aagtgggtga tgcgctcgca gctcaatgcg
     3301 gacggcacgc aattgttcgc ctatctcacc attcccgaga acgcccggat cggcgtctgc
     3361 gcgcacgagc tcggtcatct gctgttcggc ttcccggatc tctacgacac ggattcgtcg
     3421 tcggagggcg tcggcaactg gtgcctgatg ggtggcggct cctggaatgg cggggggcag
     3481 acgccggccc atccatcggc ctggtgcaag gccaaccagg gctgggcgac cgtcaccaat
     3541 gtgacctcga acggggtgcg ctcgatccag gcggtcgaga ccggccacga gatcttccgg
     3601 ctgtggaagg acggtggtgc cagccaggag tatttcctgg tcgagaaccg gcagaaaacc
     3661 ggcttcgaca gcgggctgcc cggcgagggc ttgtgcatct ggcacatcga cgaagcgatc
     3721 tcgaccaact cgaacgaggc gcatccgaag gttgccctgg agcaggccga cggcaaggac
     3781 gatctgggca cggccgccaa tcgtggcgac gccggcgatg tctacccggg ctccgccggc
     3841 aagacgatct tcgacaacgg ctcgtcgccc ggctcgcgtt cctatgcggg caacgacacc
     3901 tgtgtcgccg tcgcctcgat ctccgccagc gggccgacga tgaccgcgga cctgcgggtt
     3961 cgctgcggca agtcggtggt caaggacgtc agcgacaagg ggcgcgacaa gcggctcgag
     4021 aagcgccccg acaagatcgg cgagaagcga cccgacaagc tctgggacaa gtcggtcgtc
     4081 gaggacaagc ggcccgacaa gccggagatc gacaagcgca gcggctatga caagggctgg
     4141 gactacgact accgtgaccg tttcggccgg gcaccggagc ggcatggcga tctcgagcgg
     4201 cgggtcgagg cgctcgaatc ggcgttcggc ggcctcgcac cgttcatcga cagctcgctg
     4261 cgacccgacc tcgagggcag cggcctgagc gccgaggagg acctcgcgcg gatgcgcgac
     4321 gagatggagc agcagacgca gagcgacaag cggctgctcg attcgccgac gcaccgtcgc
     4381 tgaggaggcc gccgaggtcg accttggggt cggagcaggc cgcgggcacg atgcgacggt
     4441 cgattgtcgt gctcgcggat cccgacgacc ggggagcgct gctggttgcc aggtccctgc
     4501 ggctgcgcgg tgcgccggtg gcactggtcg cgaccgacca gttgctgctg gcgccggtct
     4561 gggagcacga cccgctgggg gcgagccggg tcgttctcgg caacggcctg gagctcaccg
     4621 attcgtcgat cggtgtcctg ttctgccgtg ctcgcggcgt cgacccgccg caattccgtg
     4681 gcgctgtgga aagcgatcgg cgctacgcgc aggaggagtt cttcgcgctg ctcatgagct
     4741 ggctggcccg gctcggcgag cgggtcctca atcggccgca tcccggcaat ctgcacggca
     4801 cccagtgcag ccttgtcgag gacctgctga gctcggcgtg gtccaggggc tcgccgcacg
     4861 ggttcgcggc cgccagtcat gcccgccatc tgccgcccgg cgccgggcct gtccgcgaat
     4921 accgaccctg gggtggtcga ttcgatgccg ggcaaccggc cgggagcggt gtgcccgggt
     4981 cggtggtcgc gggtggcccc ggcatgcggc tgccgccgct gcctgacggg cggcactgga
     5041 acgtgaccgt gatcggcgat cgcctgctcg gtgggcagcc gccggcggcg gctcgctccc
     5101 tggctttccg gctggtgcgg gaccgcgggc tgagctttgc cgcgatcgag ctcttccagc
     5161 tcgccgatgg cgggctcggc catggcatga tcgatccctt tccggcgctg gaccacgccg
     5221 gggagatcga ggcggctgcc gatcatctgg ctctggcgtt gtccaggtgt gtcgcatgat
     5281 cgtgctttgc ggcattccga gcgagccgcc gatgcgcatg gtcgaggatg cggcagcgcg
     5341 gctgggcgtg gagacccatc acctcaatca gcgcgatgcg gcgaccaccg ccctgtcgat
     5401 cgatgccacg ggcaatgggc tcgcctttcg ccagcacggg cctgacgggt cgcgcgatct
     5461 ggctgccgcg agcggcatgt tcgttcgatt gaccgactgg cgggtcctgc cggagtatcg
     5521 gcgcgctggc ggcaccgcca tgcaggatcg cctgcggatc gaggcctggc acggcctgct
     5581 cggcgactgg ctggagacca cgcccatgct ggtcatgaac cggaccaagc cgtcgaacag
     5641 caacatgtcg aagccctatc aggcacaggt gatcgccgcc gccggctttc gcgttccagc
     5701 gacgatcgtc accaaccagg tggcggtggt gcgtgccttc aaggcccggc atcgccgggt
     5761 gatcttcaag tcgatcagcg cgcatcggtc gatcgtcacc gagctcaccg aagaggccga
     5821 gcgccacctc gagcgcatcc gcctgctgcc gacgcagttc caggagctga tcgaaggcga
     5881 cgacgtgcgg gttcacgtcg tcggggcaaa gctgctcgcg acgcggatcc gcagccgcgc
     5941 catcgactat cgctatgccg gcggggagca gggtggtgcc atcttcgagc cgatgacgct
     6001 gccggaggtg gttgcggagc gatgcctggg tctgtcgcgc cgcctcggcc tgccgctctg
     6061 cggcatcgat ctcaagctga cggtcgatgg cgaatacgcc tgcttcgagg tcaacccggc
     6121 gcctgccttc agcagcttcg aggagcggtc ggggcagccg atcgccgagg cgatcgctcg
     6181 cgccctcgcc ggcatttgat gggaagccgc aggttggaat gcgtccgggc gcaccttact
     6241 tccgcaaggc cgggacttcc gcaaggccgg gcaatgagag gagacctgcg tcatggtttc
     6301 gatcgtcgag aactgggccg tgatcatcgg cacggtcata ggcatcgttc aggcgggcgt
     6361 ggcgcccggc acggcacggc tcgaggttcg gatcgagcgc atcgagccgg tcgagagcta
     6421 cccgatgctg gtcacgcaga agccggggga tcggatcagc atcagcgtcc cgaacggcaa
     6481 ggtcggcgag gggctgttga acgcgccgat cgaggtcagg gtgcggcgtg gccgcgatcc
     6541 cgaccttgtg ttcgccgcac cggactggaa tccggcgcct cggtgagctt gtccggtgcc
     6601 gtcaacgctc agtgaggaac ctttccatgc atctcgcctg aaggctgctc gtcgccgtgg
     6661 ttctgctgct ccaggcggca ccggcccatg ccgtcgccaa tccggcggcg gtcttctgcg
     6721 tggagtcggg cggccgctac gagatgcggc aggaggcgaa tggcacgcgc gggatgtgca
     6781 tcctgccgga tggcgagccg atcgacgcct ggaagcattt ccgccgggag caggggcagc
     6841 cggcgcagca gcgcccggcg gggggcctga ttgctctcga ccgttccgac cgtcgagagg
     6901 ctcacagcac ggctgcggcc agcgaccaga gggtgatgaa gaacaggatc agggagccgg
     6961 gcgtccacag catggcgcgc atgtcgtggg tctgggctgt gaggtaggag gcgaagtgca
     7021 gcagccgcga taccacgtag ccatagagca ggatctgggc gagcagcagc ggcggtcccg
     7081 tgagcacgaa gatcagcccc gccacgagga aatagggcag gctctcgagg tcgttctgct
     7141 ggatgcggcg gatgcgctcc acgcggtcgt tcggttcgag ctggcccggg ttcggcgccg
     7201 ggttgagcga tgtcttcctg aggtcctccg gtgaacggaa gccgcccttc tcctgcatca
     7261 tccgcaccac ggtcagccat ggcatgcaga tggccttgag gatgatgagc gtggcggcaa
     7321 tgacgtaggt ggcgacgagc gggtcggcga ggtcgagggc agtcatgggc acttgtcctc
     7381 cgcgtggcgg gcaatgacgg tcgtgcagtt tcatttgccc cgggtcactg cttgcaaata
     7441 aaaaatcgag aaagtcaatc acgacgtaat agccggttgc cggggctggc gaggacgaag
     7501 cagccattgg cgcgatcgtg ctccacgtcg cagcgattgt cgcgacagcc ctggcgaacc
     7561 gggtgccggg caatgtgcac ggcatgttga cggcgggttg catttgcgct tctggctcgc
     7621 tgccggcagg gctggcgctg acgaaggggg cgagagactg atgaagatcc ggaccatcga
     7681 gacgctggcg ctcaaggtgc cgatcggggc gaagacgttc tattcgtccc aggcgcggtt
     7741 tccggagcgc aactcgctgc tcgtgaagat caccaccgat gacgggctcg tcggctgggg
     7801 cgagggtggc cagtacgggc cgggcgagcc cgtggcgtct gcggtcgtcg atgtgctggg
     7861 gccgcggctg atcggccgac gggcggacca gcccgtgcgc atctgggagg acaactacag
     7921 cttcagccgc gatttcggcc gcaccggcac gtatgtcgag gcgatgagtg cgatcgacat
     7981 cgcgctctgg gacatctggg ggaagtcgct gggccggccg gtgcaccatc tcatgggcgg
     8041 tgccttccgc gaccgggcca tggcctacgg cacgggctgc tactatccgg agttctttcg
     8101 cgacacgccc cggatgatgg aggcgctggc ggaggaggtc gcgggctacg aggcggccgg
     8161 tgtgaccgcg gtcaagatca agctcggcct cctctccgtc gccgaggacc gggcccggat
     8221 cgagctcatc cgcaacacgg tcggccccgc cgtgcggctg atggcggacg ctaaccacgc
     8281 ctacaacgcc gccacggcgc tgcgcatcgg ccgggtcatg gacagccacg atttcctctg
     8341 gttcgaggag ccggtgccgc ccgaggacca cgacggctat cgcctgctgc gcacgaagct
     8401 ggacacaccc attgccggcg gcgagtgcga gttcgcccgg ttcggctttc gcgacttcat
     8461 cggcaagggc tgcgtcgaca tcgtccagcc cgacatctgc gcctcgggcg gcttcagcga
     8521 gttcgccaag atcctggcca cggccggcca gcacggtgtc ctgaccatcc cgcatgtctg
     8581 gggctccggc gtcgccgtgg ccgcggccct gcaggcgatc gccaccatcc cgcccatgcc
     8641 gcacacggcg gcccccgtca ccctgcagaa cgagccgatg gtcgagtacg accgcaccat
     8701 gaacccgctc cgcgacggca tcctgcacga gaacttcaag ctcgaggacg gggcggtcgc
     8761 gatccccgac gggccggggc tgggcatctc ggtcgatgaa ggggcgctgg cggggtatgt
     8821 ggcgcagcgg cgggtcgtgg aggggtaggc cagatcgaca gatggagctg aggatcgcga
     8881 tgccgcccga gcccctgccg ccgcccacag caccccgcgt cgcgcggctg ccgaagaacc
     8941 cgatcatccg gccgcacatg gatggccgga tgggcgacaa catcaacggg ccgtccctga
     9001 tccgggtgcc ggactgggtg gagggggcac tcgggcggta ctatctgtat ttcgggcatc
     9061 atgacggggc ctatatccgt ctcgccttcg ccgatgatct ggccgggccg tggcgcatgc
     9121 acgagccggg cgtgctgccg ctggcggcgt cgggcttcgc cgggcatctg gcctcgccgg
     9181 acgtgcatgt ggatgacgag gagcgacgat ttcgtctttt cttccacggc tcggacacgg
     9241 ccacgggccg cggtggcgag cagcggacgc gcctggcgct gtcgcgggac gggctgcgct
     9301 tcgaggcccg ggaagagacg ctgggggtgc cctactggcg cgtgttccgg catggcggct
     9361 ggcattatgc gctgggcatg ccgggccggc tctaccggtc gggggacggg gtctccgggt
     9421 tcgaggccgg gcctgtggtg ttcgcgcccg gcatgcgcca ttcggcggtg gcggtcgatg
     9481 gcgatacggc gttcgtctac tacactcgga tcggcgaccg ccccgagcgc atcctgctcg
     9541 ccaccatgga cctgcgcccg ccctggacgg actggcgacc gagcgaggcg gtcacggtgc
     9601 tggcaccaga ggaggcctgg gagggcgccg acctgccgct cgagccgtcg cgcgagggca
     9661 tcgtccgggg gccggtccgg caattgcgcg acccggcgat ctttcgcgat gggggacgta
     9721 cctggttgct ctacagcgtg gcgggtgaat ccggcatatc aatggccgag atttcagggt
     9781 gacatgttgc gttgaccgtg ccggtgccga agcccagccg ccacgcccgc cgctgcccgg
     9841 tcgtcattcc tcgaggagag cccgtatgcg ccgcttttcg tccttcctgc tggccggcct
     9901 cgccctcgcc ggctgcacat ccgtcgggca ggtcggtctg atgacgcggc ccggcgcgga
     9961 tgtaggggcg atcgtcaggg aggcgcggcc ctacaaggag ctgggggcgg tcgagggcga
    10021 ggcctgtcgc aacttcctgc tcggcatcat tccctggggt gattcgagtg cgtcgacggc
    10081 catggcgaac gcgctcgaca aggcgggcgg cgacgcgctg atcaatgtct cggtcgagac
    10141 gacgctctac ggcttcatcc cgatctacaa cgtcttcgcc aatacctgca cgacggtgcg
    10201 cggcgtcgcg atcaagttcg acgaaggcga gcagcccggc gcatcggcaa gcgccgcgcc
    10261 ctgaacgtcc agcgcgccga cgtcaccagt cgccgatcag gacctgcagc cgcgcgggat
    10321 cgatgacgag gcggccgtag cgcttctcga cgcagccgtc cttggccaga gcgctgagca
    10381 gacggccgac gctgttccgc gacagagcgg ccatctcgcc gagctcgccc tggctgatcg
    10441 gcagctccac ccggctgctc ccgatctcgg gcggatcggc gaggcgggcg agggtcacga
    10501 tcagccgcag gcgggcatca ctcgaggcga gacaggcgat gtgcgacagg gcctcgtcga
    10561 gatgccggat ggtgatgccg gccgcccggc gccagatttc gggatcgtcg gcggccattg
    10621 cctcgagcgc cgacgtgggg acgtagagga tgcgggcgtc ggtgcgggcg ctcagctcgg
    10681 tgcaacgccg cctgcccgag acggccgatg atacgcccac ccaccagccc ggccgcccga
    10741 catggatgag catcggcgcg aacgggccgg gcgcgatcag cacgtcgaca tggccgctcg
    10801 ccaccccgag gagcccgccc ggcggatcgt cgagcgcatg gatcaggtcg cccgccgcca
    10861 ccgtccgcag cacgctcgag gcgagcagtt gcgtctggaa cgagacgggt tggcgggaga
    10921 gccagccgac cgtggtcacg acgcgatgcg cggcggccgg agtcacgacg gtttcccctt
    10981 caatcgcacc agcgcgtgca atctgggcgg tgaagtccta aatttgctgg aaaatgggtt
    11041 gggggtacat cgtcgctgag cg
//
LOCUS       DAOTFI010000168        10978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7095_length_10978_cov_6.336446, whole genome shotgun sequence.
ACCESSION   DAOTFI010000168 DAOTFI010000000
VERSION     DAOTFI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7095_length_10978_cov_6.336446"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="P5558_16200"
     CDS             complement(<1..261)
                     /locus_tag="P5558_16200"
                     /inference="COORDINATES: protein motif:HMM:NF037209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25911.1"
                     /translation="MDFEAYRVQSAVSETRAAIRAKHDADEADAVRQLIDQLDLSPDR
                     RKSVSEEAARLIRLVRDETDPTMMEAFLDEYGLSTGEGVGLMC"
     gene            complement(458..1471)
                     /gene="doeB"
                     /locus_tag="P5558_16205"
     CDS             complement(458..1471)
                     /gene="doeB"
                     /locus_tag="P5558_16205"
                     /EC_number="3.5.1.125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042400 - ectoine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N(2)-acetyl-L-2,4-diaminobutanoate deacetylase
                     DoeB"
                     /protein_id="HRY25912.1"
                     /translation="MRSQYLPPNPISSTVPFDEDGKHHGYLRLPCSRDVSAWGSIMIP
                     ITVVHNGAGPTALLTGANHGDEYEGPIALQRLAYDLDPGEITGRVIIVPYMNTPAFHA
                     ARRTSPLDGVNLNRCFPGRPDGSPTEKIADYFMRTLLPMADVVLDFHSGGKTLDFIPF
                     AAAHILDDANQQAACSAAMRAFNAPYSMMLREIDAVGMYDTAAEDAGKTFISTELGGG
                     GTATARSARIALRGAINILRHVGILEGELEEEPSVLIDTTEGDCFHLAEGSGMVEMLI
                     DLGEPVAKGATLARIWSAERTGAKPHTVKARMSGILAARHHPGLIQPGDCLAVVAAVV
                     EES"
     gene            complement(1471..2658)
                     /gene="doeA"
                     /locus_tag="P5558_16210"
     CDS             complement(1471..2658)
                     /gene="doeA"
                     /locus_tag="P5558_16210"
                     /EC_number="3.5.4.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890035.1"
                     /note="DoeA (degradation of ectoine A) is also called EutD
                     (ectoine utilization D); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0042400 - ectoine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydrolase DoeA"
                     /protein_id="HRY25913.1"
                     /translation="MPTPILHFSAEEYAGRLAKARRAMEAAGVEAIIVTDPSNMAWLT
                     GYDGWSFYVHQCVVLAMDGEPMWFGRGQDAQGALRTAWLDPANIVGYPDNYVQSTERH
                     PMDYLAARLEERGLGRLRIGVEMDNYWFTARAFAALQQGLPNARFSDVTALVNWQRAV
                     KSPRELDYMRTAARFVERMHNRIFEVIEPGMRKCDLVAEIYDASLRYDPAIGAGGDYA
                     AIVPLLPSGSDAAAPHLTWDDRPMRSGEGTFFEIAGVYRRYHCPLSRTVFLGKPTRTF
                     LDAEKAVLEGMEAGLEAARAGNRCEDIANAFFAVLARYGIQKDNRTGYPIGLSYPPDW
                     GERTMSLRPGDQTVLESGMTFHFMTGLWMADWGFEITESIVIGEKGPECLAKVPRRLV
                     VKS"
     gene            complement(2664..3656)
                     /gene="eutC"
                     /locus_tag="P5558_16215"
     CDS             complement(2664..3656)
                     /gene="eutC"
                     /locus_tag="P5558_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042400 - ectoine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine utilization protein EutC"
                     /protein_id="HRY25914.1"
                     /translation="MPTIRILTEAELRQAVPLDRTAVDCIEAGFRALAGGKVVMPPIL
                     SMAIPDFNGEVDVKTAYVPGVASFALKVSPGFFDNPKIGLPSTSGLMILFSARTGQVE
                     ALLLDNGYLTDVRTAAAGAVAARHLAREDASSAAILGAGMQARLQLQALCLVRPITRA
                     TIWGRDPTKAEAAAGELSSTLGIEVTAAFNARSSVAGADVIVTTTPATRPILMTDWLE
                     SGQHVTAMGSDQHGKSELEPACLARADLYVPDRLAQTRLMGELRSAIEAGIVPATREF
                     AELGAVVAGTAPGRTGADQITVADLTGTGVQDTAIATLARERAAASDAGTDFRS"
     gene            complement(3656..4654)
                     /gene="eutB"
                     /locus_tag="P5558_16220"
     CDS             complement(3656..4654)
                     /gene="eutB"
                     /locus_tag="P5558_16220"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042400 - ectoine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyectoine utilization dehydratase EutB"
                     /protein_id="HRY25915.1"
                     /translation="MTGDAFVTLAEIEAARDTIAPTIRTTPVEPSPSLSALAGVPVRL
                     KLEHHQQSGSFKLRGAANAVARLSEAERQAGVVAVSTGNHGRGLARACAEAGVRCVVA
                     MSELVPRNKVEAIRAEGAEVLISGRSQDEAQGAVDALVARGMIMIPPFDHRHVIAGQG
                     TLGLEIAEAMPELTTVLVQLSGGGLICGIAAALKALLPEVRVIGVSMARGAAMHASLA
                     AGHPVEVEELPTLADSLGGGIGLGNRHTFAMAKVLVDEVVLLDEPEIAAGIRHCYWQE
                     RQIVEGSGAVGVAALLAGKVRVDGPTMVLLSGGNIDMELHHRIVGGEDVDVAKEAA"
     gene            complement(4651..5436)
                     /locus_tag="P5558_16225"
     CDS             complement(4651..5436)
                     /locus_tag="P5558_16225"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRY25916.1"
                     /translation="MAAPPPVRLSPVAVSLAPAGAIARLGLIALATDLTLERDAARLI
                     PGTRAALHVTRVVFDNPTTPENLLSMRPRLTAAAALILPEMKLAAIGYGCTAASVVIG
                     SEAIRDAIHAARPGVPVATPPDAALEAFAAIGVRRIALLTPYLPETTAPMVAFFEGRG
                     IEVVSAHCLGIEDDRDMARVTSDVVSAAAEAADAPEAEAVFLACTALPALALIDRLEQ
                     RLKKPVVSANQALLWRLLHHANLSPAPGAWGRIFEVAPPESPA"
     gene            complement(5441..5878)
                     /locus_tag="P5558_16230"
     CDS             complement(5441..5878)
                     /locus_tag="P5558_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY25917.1"
                     /translation="MFKHILAPVDGSRNAMVALEKAAALRALTDADLTILTVFRHHSP
                     LEASLSVSRKDDPENVDDIMRAYAREVAESAKARAVALGATGARAFVRSGPVARTIVA
                     FGREHGVDLIVIGSRGLGSVEGFLLGSVSHKVSGIADCPVLLV"
     gene            complement(5903..7189)
                     /locus_tag="P5558_16235"
     CDS             complement(5903..7189)
                     /locus_tag="P5558_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY25918.1"
                     /translation="MALTIFVIMIVLLLLGFPMMIPLIAAAFVGFFTLFGGFSQLETM
                     VQQMMAGIRPASLIAVPMFIFAADIMTRGQSADRLIDMVMKFMGHVKGGLAVSTAAAC
                     TMFGAVSGSTQATVVAVGGPLRPRMLQAGYKDSFVLALIVNASDIAFLIPPSIGMIIY
                     GVVSNTSISELFIAGIGPGLLILALFSAYSVFYAIRHNVPTEPRASWGERLVAVQRAL
                     WPLGFPVIIIGGIYGGIFSPTEAAAACVLYALILEVVVFRSISIPDVYRTAQSTGLIT
                     AVVFILVAAGAAFSWVISFAQIPQAILGSIGIDQMGPYGVLFVISIAFFIACMFVDPI
                     VVILVLVPIFAPVVQSVGLDPVLVGIIITLQVAIGSATPPFGCDIFTAIAIFKRPYIE
                     VIRGTPPFIVMLLGASVALIFFPQIALFLRDLAFAK"
     gene            complement(7202..7780)
                     /locus_tag="P5558_16240"
     CDS             complement(7202..7780)
                     /locus_tag="P5558_16240"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY25919.1"
                     /translation="MTEQTRESIHASSLPGILGTIDSLISRIESVMLAVGVLLMALNT
                     IANVVGRFVFQSSLYFSEELNSILIILITFAGISYAARHGRHIRMSAIFDTLPPGGRR
                     TMMVIISLVTAVFMLGLCWYSIGYIETQAGRGRVLPALQIPIWWTLVWVPVGFFMTGM
                     QYLLTAIRNLTAKDVYLSTTVLEGYDNDETEV"
     gene            complement(7866..8882)
                     /gene="dctP"
                     /locus_tag="P5558_16245"
     CDS             complement(7866..8882)
                     /gene="dctP"
                     /locus_tag="P5558_16245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY25920.1"
                     /translation="MTMTRLLAGTATLALLAGPLLAAGAAADTWRYAFEESMDEVQGQ
                     YAQKFKEHIEANSDHEIQLFPYGTLGESADIMEQTQSGILQFVDQSPGFTGALIPEAQ
                     VFFVPYLLPTDQEKLGRFFRDSKAINEDFQDLYAEQGLELLKMFPEGEVAMTTKSPVE
                     GCDDLNEVKFRVMTNPLLVESYRAFGATPTPLPWGEVYGGLQTNIIQGQENPTFYLYS
                     NKLYEVTDYITYAGHNNFTTAVMANKDFYDGLAAEDQQVIQDAASAAFDFILDYQKGL
                     AESELAKIKEAKPEMTVTVLTDEQRACFKEAAAEVEAMYIDMTGERGREILEQMKQDL
                     AAVE"
     gene            complement(9057..10445)
                     /locus_tag="P5558_16250"
     CDS             complement(9057..10445)
                     /locus_tag="P5558_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY25921.1"
                     /translation="MTMWQPRRDELKRPVYRSLAQSLIEAIESGRMAPGTRLPTHRAL
                     AFDLGLSVQTVSRAYDELARMGVITGAVGRGSFVANGRVDPRSPWHRPPPSERVIDCS
                     MLVPVTGEIHEQRVSAVLAGLAADPPEAALFSFRPRETLRAHGELARVWLLGCGLELD
                     AERLLPTNGNTSAMTVALMTAAVPGDLVATEAMGHHTLPSLTATLGLRLAGLAMDQEG
                     VLPDAFDRLCRTTTVRALFVQPEGLGATAARMGEERRRALVALARQHDVWLIENDAWG
                     PLQPDRPPPLAALAPERSFYFTGLTKCLLPGLRIAWLVVPERLVAAARTRHLVTSWMA
                     TPLIAEIASRWIADGTAAELLRWQRQQLARRNDLAQRLLAGIGHAATPHGLHVWLPLP
                     EPWSEAAFVAHARHYCVAVAAGSSFAIGDQPRAQGIRVCLGAGSEAELEEGLTVLARL
                     ASSRPEPALLAV"
     gene            10509..>10978
                     /locus_tag="P5558_16255"
     CDS             10509..>10978
                     /locus_tag="P5558_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRY25922.1"
                     /translation="MHVALDDRDIRMLQILATEGRIAKTELARRVNLSATPCGERLKR
                     LEQCGLIRGYRTEVALARIAPHITVFVLVELEAHRADSFAVFERHIEGCEAITNAWAL
                     GGGFDYLLQVLARDIADYQRLIDGLLEARIGLERYFTYVMTKEVKSGPPPLELLL"
BASE COUNT         1749 a         3572 c         3966 g         1691 t
ORIGIN      
        1 acacatcaag ccgacgccct cgccggtcga aagaccgtac tcgtcgagga atgcttccat
       61 catcgtcggg tcggtctcgt ccctgacgag gcgaatgagc cgtgccgcct cttccgacac
      121 gctctttcgg cggtccggtg acaggtcgag ctggtcgatc agctggcgca ccgcatcggc
      181 ttcatcggcg tcgtgcttcg cacgtatggc ggcccgtgtc tcggagacgg ctgattgaac
      241 gcgatatgcc tcgaaatcca ttcctgcccc cgtttcctgc gtccggcacg tcacgcgacg
      301 aattaatcca aggtaacccc aatttcggat cggctctatc actagttggt catctgtcac
      361 acaaccgcag accgtcccga ttgccgaacg actgcttgtg ctggaccgtg gcaatggtca
      421 cgacggcagg cgatcgaagc ctctgccggc tggcggctca gctttcctcc acgacggcgg
      481 cgaccacggc caggcagtcg ccgggctgga tcaggcccgg gtggtggcgg gcggcgagga
      541 tgccggacat ccgggccttg acggtgtgcg gcttggcacc ggtgcgctcg gccgaccaga
      601 tgcgggcgag cgtggcaccc ttggcgaccg gctcgccgag atcgatcagc atctcgacca
      661 tgccgctccc ctcggcgagg tggaagcagt cgccctcggt cgtgtcgatc agcacgctcg
      721 gctcctcctc gagctcgccc tcgaggatgc cgacgtggcg gaggatgttg atggccccgc
      781 ggagcgcaat gcgggcagag cgggcggtgg ccgtgccgcc gccgccgagc tcggtcgaga
      841 tgaaggtctt gccggcatcc tcggcggcgg tgtcgtacat gccgaccgcg tcgatctcgc
      901 gcagcatcat cgagtagggg gcgttgaagg cgcgcatggc ggcgctgcag gccgcctgct
      961 ggttggcgtc gtcgaggatg tgcgcggcgg cgaaggggat gaagtcgagc gtcttgccac
     1021 cggaatggaa gtccaggacc acgtcggcca tcggcaggag ggtgcgcatg aaataatccg
     1081 cgatcttctc ggtcggcgag ccgtcgggcc ggccgggaaa gcagcgattg aggttgaccc
     1141 cgtcgagcgg cgaggtgcgc cgtgcagcat ggaacgccgg cgtgttcatg tagggcacga
     1201 tgatcacccg gcccgtgatc tcgccgggat cgaggtcgta ggccaggcgc tgcagggcga
     1261 tcggcccctc gtactcgtcg ccgtgattgg cgcctgtcag cagcgccgtc ggcccggcgc
     1321 cgttgtgcac cacggtgatg ggaatcatga tcgagcccca ggcggagacg tcccggctgc
     1381 agggcaggcg gagatatccg tgatgcttgc cgtcctcgtc gaagggcacg gtggacgaga
     1441 tcgggttggg cggcaggtat tgggagcgca tcagctcttg accaccagcc ggcgcggcac
     1501 tttggcgagg cattccggcc ccttctcgcc gatgacgatg ctctcggtga tctcgaagcc
     1561 ccagtcggcc atccacaagc ccgtcatgaa atggaaggtc atgccggatt cgagcaccgt
     1621 ctggtcgccg ggcctcaggc tcatcgtgcg ctcgccccag tccggcggat aggagaggcc
     1681 gatcgggtag ccggtgcggt tgtccttctg gatgccgtat ctggcgagca cggcgaagaa
     1741 ggcattggcg atgtcctcgc agcgattgcc ggcccgggcc gcctcgagcc ccgcctccat
     1801 gccctccagg accgccttct cggcatcgag gaaggttcgg gtcggcttgc ccagaaagac
     1861 cgtgcgcgac agggggcaat ggtagcggcg atagacgccg gcgatctcga agaaggtgcc
     1921 ttcgccggag cgcatcggcc ggtcgtccca ggtcagatgc ggggccgcgg cgtcgctgcc
     1981 ggagggcagg agcgggacga tcgcggcata gtcgccgccg gcgccgatgg cgggatcgta
     2041 gcgcaggctc gcatcgtaga tctcggcgac gagatcgcat ttgcgcatcc cgggctcgat
     2101 cacctcgaag atcctgttgt gcatgcgctc gacgaagcgg gcggcggttc gcatgtagtc
     2161 gagctcgcgt ggcgacttga ccgcccgctg ccagttgacg agggcggtga cgtcgctgaa
     2221 ccgggcattg ggcaggccct gctgcagtgc tgcgaaggcc cgggccgtga accagtaatt
     2281 gtccatctcg acgccgatcc ggagacgccc gaggccgcgt tcctcgagcc gggcggcgag
     2341 gtagtccatc ggatgccgct cggtcgactg cacgtagttg tccgggtagc cgacgatgtt
     2401 ggccggatcc agccacgcgg tgcgcaacgc accctgcgcg tcctggccgc gcccgaacca
     2461 catgggctcg ccgtccatgg cgagcacgac gcattggtgg acgtagaagc tccagccgtc
     2521 atagccggtg agccaggcca tgttggaggg gtcggtgacg atgatcgcct cgacgccggc
     2581 cgcctccatc gcccggcggg ccttggcgag gcgtccggca tattcctcgg cgctgaagtg
     2641 caggatgggc gtcggcatgg ctttcagctc ctgaaatcgg tccccgcatc gctggccgcg
     2701 gcacgctccc gggctagggt ggcgatggcg gtgtcctgca cccctgtccc ggtgaggtcg
     2761 gcgacggtga tctgatccgc acctgtgcgc cccggcgccg tgccggcgac aaccgcgccg
     2821 agctcggcga attccctggt cgccggcacg ataccggctt cgatggcact gcgcagctcg
     2881 cccatgaggc gggtctgggc cagccggtcg ggcacgtaga ggtcggcccg ggcgaggcag
     2941 gccggctcca gctcgctctt gccgtgctgg tcggagccca tggcggtgac atgctggccg
     3001 gactcgagcc agtcggtcat caggatcggc cgggtcgccg gcgtggtcgt gacgatcacg
     3061 tcggcgccgg cgacggacga gcgggcattg aacgccgccg tcacctcgat gccgagggtg
     3121 ctcgacagct cgccggcggc cgcttccgcc ttcgtcgggt cgcggcccca gatcgtggcc
     3181 cgggtgatcg gccgcacgag gcagagggcc tgcagctgca ggcgggcctg catgccggcg
     3241 cccaggatag cagcactcga cgcatcctca cgcgccagat gccgggccgc caccgccccg
     3301 gcggcggcgg tgcggacgtc ggtgagatag ccgttgtcga ggagcagcgc ctcgacctgg
     3361 ccggtcctgg cggagaagag gatcatcagg ccggaggtgg agggcaggcc gatcttcggg
     3421 ttgtcgaaga agccgggcga gaccttgagc gcgaagctcg ccacgccggg gacataggcg
     3481 gtcttgacgt cgacctcgcc gttgaagtcg ggaatggcca tggagaggat cggtggcatc
     3541 accaccttgc cgccggccag cgcgcgaaac ccggcctcga tgcagtccac ggcggtgcgg
     3601 tcgagcggca cggcctggcg aagctcggcc tcggtcagga tgcggatcgt gggcatcagg
     3661 cggcttcctt cgcgacgtcg acatcctcgc cgccgacgat gcggtggtgc agctccatgt
     3721 cgatattgcc gccgctcagc aggaccatgg tcgggccgtc gacccgcacc ttcccggcca
     3781 gcagcgccgc caccccgacc gcccccgagc cttcgacgat ctgccgctcc tgccagtagc
     3841 aatggcggat gccggccgcg atctcgggct cgtcgagcag gaccacctcg tcgaccagca
     3901 ccttcgccat ggcgaaggtg tgccggttcc cgaggccgat gccgccgccg agactgtcgg
     3961 ccagggtcgg cagctcctcg acctcgaccg gatgcccggc cgcgaggctc gcatgcatcg
     4021 ccgcaccccg cgccatcgag acgccgatca cccgcacctc gggcaagagc gccttcagcg
     4081 cggcggcgat gccgcagatc aggccgccgc ccgagagctg gaccaggacc gtcgtcagct
     4141 ccggcatggc ctcggcgatc tccaggccga gcgtgccctg gccggcgatg acgtggcggt
     4201 ggtcgaaggg cgggatcatg atcatgccgc gagcgaccag cgcatcgacg gcaccttgcg
     4261 cctcgtcctg gctgcgcccg gagatcagca cctcagcacc ctcggcgcgg atcgcctcca
     4321 ccttgttccg cggcaccagc tccgacatgg cgacgacgca gcgcacgccg gcctcggcac
     4381 aggcgcgggc gaggccgcga ccgtgattgc cggtcgagac ggcgaccacg ccggcctgcc
     4441 gctccgcctc ggagagccgg gcgaccgcgt tggcggcacc ccgaagcttg aagctgccgc
     4501 tctgctggtg gtgctcgagc ttgagccgga ccggcacgcc ggcgagagcg ctcaggctcg
     4561 gcgagggctc gaccggggtc gtcctgatcg tcggcgcgat cgtgtcccgc gcggcctcga
     4621 tctcggccag cgtgacgaag gcgtcgccgg tcatgccggg ctctccggcg gcgccacctc
     4681 gaagatgcgc ccccaggcgc cgggggcggg cgagaggttc gcgtggtgga gcaggcgcca
     4741 gagcagcgcc tggttggcgg agacgacggg cttcttaagg cgctgctcca gccggtcgat
     4801 cagggcgagg gcgggcaggg ccgtgcaggc gagaaagacc gcctcggcct cgggtgcatc
     4861 ggccgcctcg gccgcggcgc tgacgacgtc ggatgtgacc cgcgccatgt cgcggtcgtc
     4921 ctcgatgccg aggcagtggg cgctgaccac ctcgatgccg cgcccctcga agaaggcgac
     4981 catcggtgcg gtggtctctg gcagataggg tgtcaggagc gctatccggc ggaccccgat
     5041 ggcggcgaac gcctcgagcg ccgcgtccgg cggggttgcc accggcacgc ccggccgggc
     5101 ggcgtggatg gcgtcccgga tcgcctcgct gccgatcacg acggaggctg ccgtgcagcc
     5161 gtagccgatg gccgcgagct tcatttcggg caggatgagg gccgcggcgg cggtcagcct
     5221 cgggcgcatc gacaagaggt tctccggcgt cgtcgggttg tcgaagacga cccgggtgac
     5281 atgcagcgcg gcgcgggtgc cggggatcag ccgggcggcg tcgcgctcga gggtcagatc
     5341 ggtggcgagg gcgatcaggc cgaggcgtgc gatggcgccg gcgggtgcga gcgagacggc
     5401 gacgggcgag agcctgacgg gtggcggcgc ggccatcacg ctacaccagc agcacggggc
     5461 agtccgcgat gccgctcacc ttgtgggaga cgctgccgag caggaagccc tcgaccgagc
     5521 cgaggccccg gctgccgatg acgatcaggt cgacgccgtg ctcccggccg aaggcgacga
     5581 tcgtgcgggc gaccgggccg ctgcggacga aggcgcgagc cccggtggca cccagggcga
     5641 cggcacgtgc cttggcgctc tcggcgacct ccctggcgta ggcccgcatg atgtcatcga
     5701 cgttttccgg gtcgtccttg cgcgacacgg agagcgaggc ctcgagcggc gaatggtgac
     5761 gaaagacggt gaggatggtg aggtcggcat cggtcagcgc tctgagcgcc gccgccttct
     5821 ccagcgccac catcgcgttc ctcgagccgt cgaccggggc caggatgtgc ttgaacatgc
     5881 cgtctcctgt cacttggccc ggttacttgg cgaaggcgag atcgcgcagg aagagcgcga
     5941 tctgcgggaa gaagatgagc gccaccgagg cgccgagcag catgacgatg aagggcgggg
     6001 tgccgcggat cacctcgata tagggccgct tgaagatcgc gatcgccgtg aagatgtcgc
     6061 agccgaaggg cggtgtcgcc gagccgatgg cgacctgcag cgtgatgatg atgccgacca
     6121 ggacggggtc caggccgacc gactggacca ccggcgcgaa gatcggcacc agcaccagga
     6181 tcacgacgat cgggtcgacg aacatgcagg cgatgaagaa ggcgatcgag atgacgaaga
     6241 gcacgccgta ggggcccatc tggtcgatgc cgatcgagcc caggatggcc tgcgggatct
     6301 gggcgaacga gatgacccag gagaaggcgg cgccggcggc gaccaggatg aagacgacag
     6361 cggtgatcag cccggtggac tgcgcggtgc ggtagacgtc ggggatgctg atcgagcgga
     6421 agacgacaac ctcgaggatc agcgcgtaga gcacgcaggc ggcggcggcc tcggtcggcg
     6481 agaagatgcc gccatagatg ccgccgatga tgatgaccgg aaagccgagc ggccagagcg
     6541 cccgttgcac cgccacgagc cgctcgcccc aggaggcgcg cggctccgtc ggcacgttgt
     6601 gcctgatcgc gtagaagacc gaataggccg agaacaaagc gagaatcagc aggcccggcc
     6661 cgatgccggc gatgaagagc tccgagatcg aggtgttgga gacgacgcca tagatgatca
     6721 tgccgatcga cggcgggatc aaaaaggcga tgtcagaggc gttgacgatc agggccagga
     6781 cgaagctgtc cttgtagccg gcctgcagca tgcggggcct gagagggcca ccgacggcga
     6841 cgaccgtcgc ctgtgtcgag cccgagacgg cgccgaacat ggtgcaggcg gcggcggtcg
     6901 agacggcgag cccgcctttg acgtggccca tgaacttcat gaccatgtcg atcagccggt
     6961 cggccgactg gccgcgggtc atgatgtcgg cggcgaagat gaacatcggc acggcgatca
     7021 gcgaggccgg gcggatgccg gccatcatct gctggaccat ggtctcgagc tgcgagaagc
     7081 cgccgaagag cgtgaagaag ccgacaaagg ccgcggcgat gagcgggatc atcatcggga
     7141 agccgagcag cagcagcacg atcatgatga cgaagatggt gagcgccatg gccgcctttc
     7201 cctagacttc cgtctcgtcg ttgtcgtagc cctcgagcac ggtggtcgag aggtagacgt
     7261 ccttggccgt gaggttcctg atcgcggtca ggaggtactg catgccggtc atgaagaagc
     7321 ccaccggcac ccagaccagc gtccaccaga tggggatctg cagggccggc agcacgcggc
     7381 cgcggcccgc ctgggtctcg atatagccga tcgagtacca gcagagcccg agcatgaaga
     7441 cggctgtgac gagggagatg atcaccatca tcgtccttcg gccgcctggt ggcagggtat
     7501 cgaagatggc ggacatgcgg atgtgccggc cgtgccgggc cgcatagctg atgccggcga
     7561 aggtgatcag gatgatgagg atgctgttga gctcctcgga gaagtagagg ctgctctgaa
     7621 agacgaaccg gccgacgacg ttggcgatgg tgttcagcgc catcaagagg acgccaacgg
     7681 ccagcatgac cgactcgatc cggctgatca ggctgtcgat ggtgccgaga atccccggca
     7741 gggacgatgc gtggatcgac tctcgggtct gttccgtcac cggccgatat cctcgaagga
     7801 gctgccatgg gtcgaagccc cggcgtcggg cggccgccgc ccggcgccgg gtctggtacg
     7861 gcccgctact cgacggcggc gaggtcctgc ttcatctgct cgaggatctc cctgccgcgc
     7921 tcgccggtca tgtcgatgta catcgcctcg acctcggcgg cggcctcctt gaagcaggcg
     7981 cgctgctcgt ccgtgaggac ggtgaccgtc atctcgggct tcgcttcctt gatcttggcg
     8041 agctcgctct cggcgaggcc cttctggtag tcgaggatga agtcgaaggc ggcgctcgcc
     8101 gcgtcctgga tgacctgctg gtcttccgcc gccagcccgt catagaagtc cttgttggcc
     8161 atcaccgccg tcgtgaagtt gttgtggccg gcataggtga tgtagtcggt cacctcgtag
     8221 agcttgttgg agtagagata gaaggtcggg ttctcctggc cctggatgat gttggtctgc
     8281 aggccgccat agacctcgcc ccagggcagg ggcgtggggg tggcaccgaa ggcgcggtac
     8341 gattcgacca ggagcgggtt ggtcatcacg cggaacttga cctcgttcag atcgtcacag
     8401 ccttcgaccg gcgacttggt ggtcatcgcc acctcgccct cggggaacat cttcagaagc
     8461 tccaggccct gctcggcgta gaggtcctgg aagtcctcgt tgatcgcctt gctgtcgcgg
     8521 aagaaccggc cgagcttctc ctggtcggtg ggcaggagat aggggacgaa gaagacctga
     8581 gcctcaggga tgagcgcacc ggtgaagccc ggcgactggt cgacgaactg gaggatgccg
     8641 gactgcgtct gctccatgat gtcggcggat tcgccgagcg tgccgtaggg aaagagctgg
     8701 atctcgtggt cgctgttggc ctcgatgtgc tccttgaact tctgggcgta ctggccctgc
     8761 acctcgtcca tggattcctc gaaggcgtag cgccaggtgt cggcggcggc cccggcggcc
     8821 agcaaggggc cggcgaggag ggcgagagtg gccgtgccgg ccaggaggcg tgtcatcgtc
     8881 atgggtggct cccctaaggc attcttgtat gcagcatcgc caatcgacca cgaccggtag
     8941 tgttccggcg acggagactg gcgttgcccg attgcctgta agctttgtga gactcgatac
     9001 cgtgtcaatt gctttattgc atcatgacaa tcgccggggc tcgacttgag cgctcttcaa
     9061 accgccagca gcgccggctc cggtcggctg ctggccaggc gcgccagcac cgtgaggccc
     9121 tcctccagct cggcctcgct gccggcgccg aggcagacgc ggattccctg ggcccgcggc
     9181 tggtcgccga tggcgaagct cgacccggcg gcgacggcga cacagtagtg tcgggcatgg
     9241 gcgacgaacg ccgcctcgct ccatggctcg ggcaagggca gccagacatg caggccatgg
     9301 ggcgtcgccg cgtggccgat gccggcaagg aggcgctgcg ccaggtcgtt tcgccgagcg
     9361 agctgctgcc gttgccagcg gagcagctcg gccgccgtgc catcggcgat ccagcgggac
     9421 gcgatctcgg caatcagcgg cgtcgccatc cagctggtca ccagatggcg cgtccgggcg
     9481 gccgcgacca ggcgctcggg cacgaccagc caggcgatgc gcaggcccgg caggagacac
     9541 ttggtgaggc cggtgaagta gaagctgcgc tcgggcgcca gggccgcgag cggcggcggg
     9601 cgatcgggct gcaggggccc ccaggcgtcg ttctcgatga gccagacgtc gtgctggcgg
     9661 gcgagcgcca ccagcgcccg gcggcgttcc tcccccatcc gggccgcggt ggcaccaagc
     9721 ccctccggct gcacgaagag cgcccggacc gtcgtcgtcc ggcagagccg gtcgaaggca
     9781 tcgggcagca caccctcctg gtccatcgcc agcccggcga ggcgcaggcc gagcgtcgcg
     9841 gtgagcgagg ggagcgtgtg atggcccatc gcctcggtgg cgacgagatc gcccggcacc
     9901 gccgccgtca tcagcgccac ggtcatggcc gaggtgttgc cgttggtcgg cagcagccgc
     9961 tcggcatcga gctcgaggcc gcagcccagg agccagaccc gcgccagctc gccatgcgcc
    10021 cgaagcgtct cgcgcggccg gaacgagaag agcgccgcct cgggcgggtc ggcggcgagc
    10081 ccggccagca ccgccgatac ccgctgctcg tggatctcgc cggtgaccgg caccagcatg
    10141 gagcagtcga tcacgcgctc gctcggcggc ggccggtgcc agggcgagcg cgggtcgacc
    10201 cgcccgttgg cgacgaagct gccgcgcccg accgccccgg taatgacgcc catccgggcc
    10261 agctcgtcat aggcgcggct gacggtctgc acgctcaggc cgagatcgaa ggccagcgcc
    10321 cggtgcgtcg gcagccgcgt gcccggcgcc attcgcccgg actcgattgc ctcgatcagg
    10381 ctctgcgcca gggaccggta gaccgggcgc ttcagctcgt ccctgcgcgg ctgccacatt
    10441 gtcatgagac attcttgccg cggcttgggc cgattgacaa gccgccggcg gcgggcctag
    10501 gcccagacat gcacgtcgcc ctcgacgacc gcgatatccg gatgctgcag atcctcgcga
    10561 ccgaagggcg gatcgcgaag accgagctgg cacgccgggt taatctctcc gccacgccct
    10621 gtggcgagcg gctgaagcgg ctggagcagt gcggcctgat ccgcggctat cggaccgagg
    10681 tggcgctcgc ccggatcgcc ccgcacatta ccgtcttcgt gctggtcgag ctcgaggcgc
    10741 atcgcgccga cagctttgcc gtgttcgagc ggcatatcga aggctgcgag gcgatcacca
    10801 acgcctgggc gctcggcggc ggcttcgact acctcctgca ggtgctggcg cgggacatcg
    10861 ccgattatca gcgcctgatc gacgggctgc tcgaggcgcg gatcgggctc gagcgctact
    10921 tcacctatgt catgaccaag gaggtgaagt ccgggccacc gccgctcgaa ctgctgct
//
LOCUS       DAOTFI010000169        10894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7164_length_10894_cov_6.138850, whole genome shotgun sequence.
ACCESSION   DAOTFI010000169 DAOTFI010000000
VERSION     DAOTFI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_10894_cov_6.138850"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="P5558_16260"
     CDS             <1..213
                     /locus_tag="P5558_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25923.1"
                     /translation="TSALRMGEAAALAPLGYLRLLLMGVVGYWLYAEVPSLATVLGAL
                     LVVGSASYTLHRNAKRQEAIREEGPG"
     gene            295..1317
                     /locus_tag="P5558_16265"
     CDS             295..1317
                     /locus_tag="P5558_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRY25924.1"
                     /translation="MMRRTSMLLAGLLLALMGTGPLAAQTYKDEYRVSTVVPAPFPWG
                     LAAERWAELVGERTDGRIKLVIYPGAQLVQGEQTREFAALRRGLIDMAVGSTINWSPQ
                     IVEMNIFSMPFLMPDYAALDALTQGPVGEVIFERIRENGVVPLAWAENGFREVTNSKV
                     AIRTPADMAGLKMRVVGSPIYNDIFTALGANPTQMSWADAQPALSTGAVDGQENPLTI
                     YDVLKMWELGQTNVTLWRYVADPLIFAVNQEVWDSFTPEDQEIVATAAVDAGAYGVEV
                     ARRGLTDEDQSLIEEIEGHGVTVVRLTDEERQAFVDATRPVYEAWREKVGADLVKQAE
                     ESVAQR"
     gene            1321..1845
                     /locus_tag="P5558_16270"
     CDS             1321..1845
                     /locus_tag="P5558_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY25925.1"
                     /translation="MAGERPAPAEHGAATPGPRVPVRLEEALAAAAMALICLISLANV
                     VVRYLTNVSFAFTEEYSVFLLVFMTFVGASAAFATNEHIRITWLLERLPRRLAWLAEL
                     ATLAVTTALFALVVYFGGQVAFDEWHWGETSPGLGHPSWVYSIWLPVLAVAIILRVLG
                     RGWRDLGRGGGARR"
     gene            1829..3148
                     /locus_tag="P5558_16275"
     CDS             1829..3148
                     /locus_tag="P5558_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY25926.1"
                     /translation="MVLDANLLLVLVFLALLAIGTPIAVALGAGGVAGILVGLDMAAL
                     GTVGTNTWSAVAKYPLIAVPLFILTGMVFERSGVAARLVTFAEACIGPRRGGLAVVAI
                     LVCLIMGGMSGSGPADAAAVATVMIPAMHKAGYPRPFSAAVIAAGASTAILIPPSIAL
                     IVYAVLVPGTDLRALFAAGLVPGLLVGVAIGVPALLLSRRHGFGSVAEVGAGAAGRPP
                     FWQSLWRALPGLLAPVIILGGLRSGLFTPTETAVVAVAYGLLVGTLVYRSLGWADIYA
                     VLVESAKISAVLMIIIAFAGIFAWAGATLGAFRMAAEALLAVSGHELVVLLLVMLMLL
                     VAGMLLDGISIYLITLPLLVPIATALDWSFVWFGVVMAITIAMGQVTPPVAVNLMVTS
                     RLAGVPMESTFVWIGWLLLAMTAALGLVIGFPELALWLPRVLGYRIT"
     gene            3242..5554
                     /locus_tag="P5558_16280"
     CDS             3242..5554
                     /locus_tag="P5558_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY25927.1"
                     /translation="MNEMDPLAGFVPKFGVGQSVTRKEDPRLLRGDGRYTDDFLPEGQ
                     LTALFVRSSHAHAMLEKLDVEAARAAPGVVMVLTTAELDAKGYPDLPCGLPFKSADGS
                     PMIVPPHPSLARGRVRYVGQPYAVVIAETMAAARDAAERIEAEFSPLPVLVELERARD
                     ADAPLLHEEAPRNTALDFAFGDEAAVEAAFQRAAHVTRLKIVNNRVMVVPMEPRGAVV
                     DYRDGRWTVRTGCQNVFGLRASLAGLVEAPVEQVRVLADDIGGSFGMKASVFPEHLPL
                     MHAARELGRPIKWVNDRSESFTTDYHGRDSVFDAELALDDEGNFLAVRVEGLGSAGAY
                     VAGFGPAIPTGVIQKNLPSLYKTPLMSVRVKLVLTNTTPMTAYRGAGRPEAVYMMERL
                     VEQAARETGRDPLELRRQNFVPPSAMPFTMASGVVCDSGDFAAVLDQGLEVADAAGFA
                     ARRQASEARGLKRGLGYCTYAEVTAPQGKEMGGIRFAPDGRITLVTGTLDYGQGHASA
                     FAQVLATRLGLPFDRIDLLQGDSDELLYGGGTGGSRSLMASGEAVLEAADQVVEKGMK
                     LASEHLEAAEADIVFEAGSFRVAGTDRRVTLAQLGIDAKGALDVELVTDTPPSTCPNG
                     CHVAEVEVDPETGRITLVGYSAVDDFGTLVNPLLVEGQVHGGVVQAIGQALTEHVVYD
                     ASGQLLTGSFMDYALPRADDLPDFKLAFHTVPATTNRLGAKGCGEAGITAALAAVVHA
                     VLDALAPYGITHLDMPLTPERVWSAIRAAG"
     gene            complement(5609..5956)
                     /locus_tag="P5558_16285"
     CDS             complement(5609..5956)
                     /locus_tag="P5558_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25928.1"
                     /translation="MGPLRGRDAQLHVLAEIAQGLQQAVGGELGDPAVHQRRHAGPVH
                     AHQAAGLGLGETPILDDRGQPTHELGAQDEVVGIIETERVKGAPLGDRDDLQLCHIDF
                     SPRGAAVPPPLVA"
     gene            complement(6043..6510)
                     /locus_tag="P5558_16290"
     CDS             complement(6043..6510)
                     /locus_tag="P5558_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HRY25929.1"
                     /translation="MTTDLYERDTHAWARDQAEALRRLAAHRPELTAELDLPHLAMEI
                     EDMGKSLARELVSRLAILLAHLAKWHWQPGERSRSWLTTIGHQRDEIAALLEDNPSLR
                     PNLDDAMAKAWSRARRQAARETGIALTVFPTACPFTLDDVLADAWLPDTTAGS"
     gene            6642..7751
                     /locus_tag="P5558_16295"
     CDS             6642..7751
                     /locus_tag="P5558_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY25930.1"
                     /translation="MAKLVRGEVWLVDLEAKVKRTDAIQAFTSQETPFVRLTDADGVE
                     GVGYSYTIGQGGSSVVALLADHLLPRLIGREAEEIERIWRDLRFSVHALQPGAVVAIA
                     LCAIDTALWDLRCKRAGLPLHVLAGGARASIPLYSTEGGWLHVEPEALVEDALAVRER
                     GFRGCKVKVGRPHVSEDIARLEAVRDAMGPGFEIFTDANQAFTPDEAIRRARAMEPLD
                     VGWFEEPLPSDDMIGHSRLCQSTSLPIAVGESIYSVRHFGQYMAERACSIVQVDVGRI
                     GGVTPWLKVAHTAEALNIPVCPHFLMELHVALCCAVPNARWLEYIPQLDELTTEGMRM
                     EGGEAFASEVPGLGIAWDMERIDRVTVEGSARVID"
     gene            7841..8857
                     /locus_tag="P5558_16300"
     CDS             7841..8857
                     /locus_tag="P5558_16300"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY25931.1"
                     /translation="MRVARMLPLAVLAAGIAGPAMASDTKVALVPGGPHPYFAAWENA
                     AAAAAADFGIEAEYRVPAAWELSKQNEMIESLVAQGFNAFLVFPGDANGTNATLEELA
                     AFDIPSAALAGCTVDPTAASFCLGTDVARSAYLGTKELIEAMGGSGRIAHFTGFLVDP
                     NTQLRIEAVEQAVAEAGGGVEIIQVIADIDAPEPADEKINAFLAARGSEVDGIVTTAW
                     VPSVVAATSLRNLGDKRIRMVGIDHDEVVLSAIEDGFVDGTMLQNPYGQGYIGSYAMD
                     LIRQGCTMKEDAPWQQTAQTAHFIDSGTAFVDADQVKDYQAAMVAVTDEIMATFKDDF
                     LVCN"
     gene            8861..9868
                     /locus_tag="P5558_16305"
     CDS             8861..9868
                     /locus_tag="P5558_16305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25932.1"
                     /translation="MATATTGRGGRGALGLLASNEVGLALLIAVLVAIFATVNPGFTS
                     PFNLFALSRVMGIDMVIGLSMMVVLVTGGLNLAVGSIGVASVMLGGWAMQVAGVPVLP
                     SMTLAVAFGAFLGWINGQLTVRMGVHSFIVTLATMSLFFGAMIVATRAEAFNALPRSF
                     VDLGKLRYGGFVSGMLPLALIVGLGLWLFFNRTSLGREILAAGANPQAAELSGVRVNR
                     TIVVAHALSGALGALAGLMLAARNGAALPSMAGHIGMGWLLPAFLAPVLGGTLLTGGV
                     VSVAGTVLGAAMVTIITNGLLQLQIGEFWVQMCLGLILLAAVLLDRARSVIAERRRSV
                     A"
     gene            9868..10815
                     /locus_tag="P5558_16310"
     CDS             9868..10815
                     /locus_tag="P5558_16310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY25933.1"
                     /translation="MLKGIWGSDWFGLFVVALVAGTGLALFQPAFLSPFNLYVLLISF
                     SLWALVAMAQMVIIAIGQMNLSVGAIGGLVAISFAGSMEVWGLPVPAAILLGLAIAAL
                     CGLLNGVMTHVTGISAFVITLATLSIFKGLNIGITEAQPFYGVPDVVKAFGNARFEGL
                     PHMLIVPVVAIILIWLLFNRMVLGRQMLALGANRHAAELSGIRVGRVVVIAHVISALL
                     AAMAGMLAVARLQLGQPTIGDDWLILSFAAPVIGGAVLAGGHISVIGTCLGVVIVTLI
                     NNALVLLGIDPFFVQLFLGGLILIAVGLNRWRERRLAPA"
BASE COUNT         1461 a         3632 c         3954 g         1847 t
ORIGIN      
        1 acctcggcgc tgcgcatggg cgaggccgcc gccctggcgc cgctcggcta tctgcgcctc
       61 ctcctgatgg gcgtcgtcgg ctactggctc tatgccgagg tgccgtcgct ggcgaccgtg
      121 ctgggagcgc tcctggtggt cggctcggcc agctacacgc tgcatcgcaa cgccaagcgg
      181 caggaggcga tccgggaaga agggccgggc tgaccggtgc tcggggcttg gcccgtgcgg
      241 cgcccccgtg gcagaaggat cgccatgaag cgaggagcat cggggaggat ggcgatgatg
      301 cgcaggacga gcatgctgct ggccgggttg ctgctggccc tcatgggcac ggggccgctg
      361 gcggcgcaga cctacaagga cgagtaccgg gtctcgaccg tggttccggc accctttccc
      421 tgggggctcg ccgccgagcg ctgggccgag ctggtgggcg agcggacgga cgggcgcatc
      481 aagctcgtga tctatccggg cgcccagctc gtgcagggcg agcagacccg cgagttcgcc
      541 gccctgcggc gcggcctgat cgacatggcg gtcggctcga ccatcaactg gtcgccgcag
      601 atcgtcgaga tgaacatctt ctccatgccg ttcttgatgc ccgactacgc ggcgctcgac
      661 gccttgaccc aggggccggt cggcgaggtg atcttcgagc gcatccggga gaatggcgtg
      721 gtgccgctgg cctgggccga gaacggcttt cgcgaggtca ccaactcgaa ggtggcgatc
      781 cgcacgccgg ccgacatggc cgggctgaag atgcgcgtcg tcggctcgcc gatctacaac
      841 gacatcttca ccgccctcgg cgccaacccg acgcagatga gctgggccga cgcgcagccg
      901 gccctctcga ccggtgccgt tgacggccag gagaacccgc tcaccatcta tgacgtcttg
      961 aagatgtggg agctcgggca gacgaacgtg acgctctggc gctacgtcgc cgacccgctg
     1021 atcttcgcgg tcaaccagga ggtctgggat tcgttcacgc ccgaggacca ggagatcgtg
     1081 gcgacggcgg cggtcgatgc cggtgcctat ggcgtcgagg tggcgcgcag ggggctgacg
     1141 gatgaagacc agagcctgat cgaggagatc gaagggcatg gtgtcacggt cgtccggctg
     1201 accgacgagg agcggcaggc cttcgtcgac gcgacccgcc cggtctacga ggcgtggcgc
     1261 gagaaggtcg gcgcggatct cgtgaagcag gccgaggagt cggtggcgca gcgctagcgg
     1321 atggcagggg agcggccggc gccggcggag cacggcgccg ccacaccggg cccccgcgtc
     1381 ccggtccggc tcgaggaggc gctggcggcg gcggcgatgg cgctcatctg cctgatctcg
     1441 ctcgccaacg tggtggtccg ctacctgacc aacgtctcct tcgccttcac cgaggaatac
     1501 tcggtctttc tcctcgtctt catgaccttc gtcggcgcct cggcggcctt cgcgacgaac
     1561 gagcacatcc gcatcacctg gctgctcgag cgtctgcccc ggcgcctggc gtggctggcg
     1621 gagctcgcga ccctcgccgt caccacggcc ctcttcgcgc tggtcgtcta tttcggcggg
     1681 caggtggcgt tcgacgagtg gcactggggc gagacctcgc ccgggctcgg ccatccgagc
     1741 tgggtctatt cgatctggct gccggtcctg gcggtcgcga tcattctgcg ggtgctcggg
     1801 cgcggctggc gagacctcgg gcggggtggt ggtgctcgac gctaatctgc tgctggtgct
     1861 ggtgtttctc gcccttctgg cgatcggcac gccgattgcg gtggcgctcg gcgccggcgg
     1921 cgtggcgggc attctcgtcg gcctcgacat ggcggcgctc ggcacggtcg gcaccaacac
     1981 ctggagtgcc gtcgccaagt acccgctgat cgccgtgccg ctcttcatcc tgaccggcat
     2041 ggtgttcgag cgctcgggcg tggcggcacg gctcgtcacc ttcgccgagg cctgcatcgg
     2101 gccgcggcgc ggcgggctgg cggtggtcgc catcctcgtc tgcctgatca tgggcggcat
     2161 gtcgggctcg gggccggcgg atgcggcggc cgtggcgacg gtgatgatcc cggccatgca
     2221 caaggcgggc tatcccaggc ccttctcggc tgccgtgatc gcggcgggtg cttcgaccgc
     2281 cattctgatc ccgccctcga tagcgctcat cgtctatgcg gtcctggtgc ccggcaccga
     2341 tctgcgcgca ctgttcgctg ccggtctggt gcccggcctt ttggtcggtg tcgccatcgg
     2401 cgtgccggcg ctgctgctca gccggcggca cggcttcggc agcgtcgccg aggtcggcgc
     2461 gggtgcggcc ggccggccgc ccttttggca gagcctctgg cgtgccctgc cggggctgct
     2521 ggcgccggtc atcattctcg gcggcctgcg cagcggcctg ttcacgccga ccgagacggc
     2581 ggtggtggcg gtggcctacg gcctcctggt cggcacgctg gtctatcgca gcctcggctg
     2641 ggccgacatc tatgccgtgc tggtcgagag cgccaagatc tcggcggtgc tgatgatcat
     2701 catcgcgttc gccggcatct tcgcctgggc cggcgccacg ctcggtgcct tccgcatggc
     2761 ggccgaggcg ctgctcgcgg tgagcggcca cgagctggtc gtcctgctcc tggtgatgct
     2821 catgctgctg gtggccggca tgctgctcga cggcatctcg atctatctca tcaccttgcc
     2881 gctcctggtg ccgatcgcca ccgccctcga ctggtccttc gtctggttcg gcgtggtcat
     2941 ggccatcacc atcgcgatgg gccaggtgac gccgccggtg gcggtcaacc tgatggtcac
     3001 gtcgcgcctc gccggggtgc cgatggagag caccttcgtc tggatcggct ggctgctgct
     3061 cgccatgacg gcagcgctcg gcctcgtcat cggctttccc gagctcgccc tctggctgcc
     3121 gcgggtgctc ggctaccgga tcacctgaac gccggatccc ggcctgcacg gggtggcggc
     3181 agcagggaaa tcagggtaag ccgcgccgga gccggcggca ggcgcaagcg ggagcgggac
     3241 gatgaacgag atggatccat tggcgggttt cgtgccgaaa ttcggcgtcg gccagtcggt
     3301 gacgcgcaag gaggacccgc ggttgttgcg gggtgatggg cgctacaccg acgattttct
     3361 gcccgagggc cagctcacgg ccctcttcgt ccgctcgagc catgcccatg cgatgctgga
     3421 gaagctcgac gtcgaggcgg cgcgcgccgc accgggcgtg gtcatggtgc tgaccacggc
     3481 cgagctggat gccaagggct acccggacct gccctgcggc ctgcccttca agagcgccga
     3541 tggcagcccg atgatcgtcc cgccgcatcc ctcgctggcg cgcggccggg tgcgctatgt
     3601 gggccagccc tatgccgtgg tgatcgccga gacgatggcg gcggcgcgtg atgcggccga
     3661 gcgcatcgag gcggaattct cgcccttgcc ggtgctggtc gagctggagc gggcgcgcga
     3721 cgcggacgcc cccctgctgc acgaggaggc gccgcggaac acggcgctcg acttcgcctt
     3781 tggcgacgag gcggcggtcg aggcggcgtt ccagcgtgcg gcgcacgtca ccaggctgaa
     3841 gatcgtcaac aaccgggtga tggtcgtgcc gatggagccg cgcggggcgg tggtcgacta
     3901 ccgggacggc cgctggacgg tgcgcaccgg ctgccagaac gtgttcgggc tccgggccag
     3961 cctggcaggg ctggtcgagg caccggtcga gcaggtccgc gtgctggccg acgatatcgg
     4021 cggctcgttc ggcatgaagg cctcggtctt cccggagcac ctgccgctca tgcacgcggc
     4081 gcgcgagctc ggccggccga tcaagtgggt gaacgaccgc agcgaaagct tcacgaccga
     4141 ctatcacggc cgcgattcgg tcttcgatgc cgagctcgcc ttggatgatg agggcaattt
     4201 cctcgccgtg cgggtcgagg ggctcggcag cgccggcgcc tatgtggcgg gcttcggccc
     4261 ggcgattccg accggggtca tccagaagaa cctgccgagc ctctacaaga ccccgctcat
     4321 gtcggtgcgg gtcaagctgg tcctgaccaa caccacgccg atgaccgcct atcgcggggc
     4381 cgggcggccg gaggcggtct acatgatgga gcggctggtc gagcaggccg cccgcgagac
     4441 cgggcgcgat ccgctcgagc tccggcggca gaacttcgtg ccgccgtcgg ccatgccctt
     4501 caccatggcg tcgggcgtgg tctgcgacag cggggatttc gcggccgtgc tggaccaggg
     4561 gctcgaggtg gccgacgcgg ccggcttcgc cgcacggcgc caggcgagcg aggccagggg
     4621 gctgaagcgc ggcctcggct actgcaccta tgccgaggtg acggcacccc agggcaagga
     4681 gatgggcggc atccgcttcg ccccggacgg acggatcacc ctcgtcaccg gcacgctgga
     4741 ctacggccag ggccatgcct cggccttcgc ccaggtgctg gccacccggc tcggcctgcc
     4801 cttcgaccgg atcgatcttt tgcagggcga cagcgacgag ctcctctatg gcggcggcac
     4861 gggcggctcg cgctcgctga tggcgtccgg cgaggcggtg ctcgaggccg ccgaccaggt
     4921 ggtcgagaag ggcatgaagc tggcttccga gcatctcgag gcggccgagg ccgacatcgt
     4981 cttcgaggcg ggctcgttcc gcgtcgccgg caccgaccgg cgggtgacgc tggcccagct
     5041 cggcatcgac gccaagggtg ccctggatgt cgagctcgtg accgacaccc cgccctcgac
     5101 ctgcccgaac ggctgccacg tggcggaggt cgaggtcgac ccggagaccg ggcggatcac
     5161 cctggtcggc tacagcgcgg tcgacgattt cggcacgctg gtcaaccctc tcctggtcga
     5221 gggccaggtg catggcgggg tggtccaggc gatcggccag gcgctgaccg agcacgtcgt
     5281 ctacgacgcc tcgggccagc tcctgaccgg cagcttcatg gactacgccc tgccgcgcgc
     5341 cgacgatctg ccggacttca agctcgcctt ccacaccgtg ccggcgacca ccaaccgcct
     5401 gggtgccaag ggctgcggcg aggccggcat caccgcggcc ctcgccgccg tggtccacgc
     5461 cgtgctcgac gcgctggcgc cttatggcat cacgcatctc gacatgccgc tgacgccgga
     5521 gcgcgtgtgg agcgccatcc gggcggcagg ctagacctcg tcatcccggc cgcaggctgc
     5581 gccgagattc ggggcggcct gcggctggtc aggctacaag gggtggcgga accgcggcac
     5641 ctcttgggga gaagtcgata tggcagagct ggaggtcgtc acgatcgccg aggggagcgc
     5701 ctttcacgcg ctcggtctcg atgatgccga cgacctcgtc ctgcgcgccg agctcatgcg
     5761 tcggctggcc gcgatcatcg aggatcgggg tttcacccag gcccaggccg gcagcctgat
     5821 gcgcatggac cggccccgcg tgtcggcgct gatgaacggc aggatcacca agttctccac
     5881 cgaccgcctg ctgcaggccc tgagcgatct cggccaggac gtggagctgc gcatcacgcc
     5941 ctcgaagggg gcccaagggc cgggtcagtg tcggggccta gtccgcgttc ctggatctgc
     6001 cggatcgcgt caaaggatgc gcaccggaaa ggatggaagc gtctatgagc cggccgtcgt
     6061 atccggcaac cacgcatccg cgagcacgtc gtcgagcgtg aacggacaag cggtcgggaa
     6121 gaccgtgagg gcgatgccgg tttcgcgcgc tgcctggcgg cgggcgcggg accaggcctt
     6181 ggccatggcg tcatcgaggt tcgggcgcag gctcggattg tcctctagga gggcggcgat
     6241 ctcgtcacgt tgatgaccga tggtggtgag ccagctgcgg ctgcgctcgc ccggttgcca
     6301 gtgccatttg gcgaggtggg cgaggagaat ggcgagccgg ctgacgagct cgcgggcgag
     6361 ggacttgccc atgtcctcga tctccatggc gagatggggg agatcgagct cggccgtgag
     6421 ctcgggacga tgggcggcca gccgtcggag tgcttcggcc tggtccctgg cccaggcgtg
     6481 ggtgtcgcgc tcgtagagat cggtggtcat ctgccttctc acgtttggaa ccatgtcatg
     6541 atagctgatc gcggggcgtc ggcgaagcat ggcggagggc tggccctgac cttcgccagc
     6601 ggctaaccag ttcagcgagg tgacaacggg aggacgggca catggcgaag ctggtgcggg
     6661 gcgaggtctg gctcgtggac ctcgaggcca aggtgaagcg gacggatgcg atccaggcgt
     6721 tcaccagcca ggagacgccg ttcgtccggc tgaccgatgc ggacggtgtg gagggggtgg
     6781 gctacagcta caccatcggc cagggtggca gctcggtcgt ggccttgctc gccgatcatc
     6841 tcctgccccg gctgatcggc cgggaggcgg aggagatcga gcggatctgg cgcgatctgc
     6901 gcttttccgt gcatgcgctg cagccggggg cggtggtggc gatcgccctt tgcgccatcg
     6961 acacggctct ttgggatctg cgctgcaagc gggccgggct gcccttgcat gtgctggcgg
     7021 gcggggccag ggcctcgatc ccgctctact cgaccgaggg cggctggctg catgtcgagc
     7081 cggaggcgct ggtcgaggac gcgctggcgg tgcgcgagcg gggctttcgc ggctgcaagg
     7141 tcaaggtcgg ccggccgcat gtgagcgagg acatcgcccg gctcgaggcg gtgcgcgatg
     7201 cgatggggcc gggcttcgag atcttcaccg acgccaacca ggccttcacg ccggacgagg
     7261 cgatccgccg ggcccgggcg atggagcctc tcgatgtcgg ctggttcgag gagcctttgc
     7321 cgtcggacga catgatcggc cacagccggc tctgccagag cacgtcgctg ccgatcgcgg
     7381 tcggcgaatc gatctactcg gtccggcatt tcggccagta catggccgag cgggcctgct
     7441 cgatcgtcca ggtcgatgtc gggcggatcg gcggcgtcac cccctggctc aaggtggcgc
     7501 acaccgccga ggcgctgaac atcccggtct gcccgcattt cctcatggag ctgcacgtgg
     7561 cgctctgctg cgccgtgccc aatgcgcgct ggctcgaata catcccgcag ctcgacgagc
     7621 tgaccaccga ggggatgcgg atggaaggtg gcgaggcctt cgcgtcggag gtgcccgggc
     7681 tcggcatcgc ctgggacatg gagcggatcg accgcgtcac ggtcgagggc tcggcgcggg
     7741 tgatcgactg aaggcgagcg aaaacgcgga acggccgttg tctccgttgg acggttcggc
     7801 catagatgcg gtccagtcgg gtcattcagg gagaattgtc atgagagtcg caaggatgct
     7861 gccgctcgcg gtgctggcgg ccggtatcgc cgggccggcc atggcgagcg acaccaaggt
     7921 ggcgctggtg ccgggggggc cgcaccccta tttcgccgcc tgggagaatg cggcggcggc
     7981 cgccgccgcg gatttcggca tcgaggccga gtaccgcgtg ccggcggcct gggagctctc
     8041 caagcagaac gagatgatcg agagcctggt ggcgcagggc ttcaacgcgt tcctggtctt
     8101 cccgggcgac gccaacggca ccaatgccac gctcgaggag ctggcggcct tcgacatccc
     8161 gtcggcggcg ctggccggct gcacggtcga cccgacggcg gcgagcttct gcctcggcac
     8221 ggacgtggcg cgctcggcct atctcggcac caaggagctg atcgaggcga tgggcgggtc
     8281 gggcaggatc gcccacttca ccggcttcct cgtcgatccc aacacgcagc tccgcatcga
     8341 ggcggtcgag caggcggtgg ccgaggcagg tggcggggtc gagatcatcc aggtgatcgc
     8401 cgacatcgac gcgccggagc cggcggacga gaagatcaac gccttcctcg cggcgcgcgg
     8461 ctcggaggtc gacggcatcg tgaccacggc ctgggtgccg tcggtcgtcg ccgccacgtc
     8521 cttgcgcaat ctcggcgaca agcggatcag gatggtcggc atcgaccatg acgaggtggt
     8581 gctgagcgcc atcgaggacg gcttcgtcga tggcaccatg ctgcagaacc cctatggcca
     8641 gggctatatc ggctcctacg ccatggacct gatccggcag ggctgcacga tgaaggagga
     8701 tgcgccctgg cagcagacgg cgcagaccgc gcacttcatc gacagcggca cggccttcgt
     8761 cgatgccgac caggtgaagg actaccaggc cgcgatggtc gccgtgacgg acgagatcat
     8821 ggcgaccttc aaggacgact tcctggtctg caactgaccc ttggcgacgg cgacgaccgg
     8881 gcgaggcggg cggggggcgc ttgggctcct cgcctccaac gaggtcggcc tggcgctcct
     8941 gatcgccgtg ctggtggcga tcttcgccac ggtcaacccg ggcttcacct cgcccttcaa
     9001 cctcttcgcc ctcagccggg tgatgggcat cgacatggtc atcggccttt cgatgatggt
     9061 cgtgctcgtc accggcgggc tgaacctcgc ggtcggctcc atcggcgtcg cctcggtcat
     9121 gctgggcggc tgggcgatgc aggtcgcggg cgtgccggtg ctgccctcca tgacgctcgc
     9181 cgtcgccttc ggcgccttcc tggggtggat caacggccag ctcaccgtgc ggatgggggt
     9241 gcacagcttc atcgtcacgc tggcgaccat gtcgctcttc ttcggggcga tgatcgtggc
     9301 cacccgggcc gaggccttca acgcgctgcc gcgctccttc gtcgatctcg gcaagctccg
     9361 ctatggcggc ttcgtctccg gcatgctgcc gctcgccctc atcgtggggc tcgggctctg
     9421 gctcttcttc aaccgcacca gcctcggccg cgagatcctg gcggccggcg ccaacccgca
     9481 ggcggccgag ctctccggcg tgcgcgtcaa ccggacgatc gtcgtggcgc acgcgctctc
     9541 cggcgcgctc ggcgcccttg ccgggctcat gctggcggcg cgcaatggcg cggccctgcc
     9601 gtccatggcc gggcatatcg gcatgggctg gctgctgccg gccttcctcg ccccggtgct
     9661 cggcggcacg ctcctgaccg gcggggtggt ctcggtcgcg ggcaccgtgc tcggcgccgc
     9721 catggtgacg atcatcacca acgggctgct gcaactgcag atcggcgagt tctgggtgca
     9781 gatgtgcctc ggcctcatcc tgctcgccgc cgtgctgctc gaccgcgcgc gcagcgtcat
     9841 cgccgagcgc cgccggagcg ttgcctgatg ctgaaaggca tctggggcag cgactggttc
     9901 gggctcttcg tcgtggccct ggtcgccggg acggggctcg ccctgttcca gccggccttt
     9961 ctctcgccct tcaatctcta cgtgctcctg atcagcttct cgctctgggc gctggtcgcc
    10021 atggcgcaga tggtgatcat cgccatcggc cagatgaacc tctcggtcgg cgccataggc
    10081 gggctggtgg cgatctcgtt tgccgggtcg atggaggtct gggggctgcc ggtgccggcg
    10141 gcgatcctgc tcggcctcgc catcgcggcg ctttgcggcc tcctcaacgg ggtgatgacc
    10201 catgtgaccg gcatcagcgc cttcgtcatc acgctggcga cactctcgat cttcaagggc
    10261 ctcaatatcg gcatcaccga ggcgcagccc ttctacggcg tgcccgacgt ggtcaaggcc
    10321 ttcggcaatg cgcgcttcga gggcctgccg cacatgctga tcgtgccggt ggtggcgatc
    10381 atcctcatct ggctcctctt caaccgcatg gtgctgggcc ggcagatgct cgcgctcggg
    10441 gccaaccggc atgccgccga gctttccggc atccgggtcg ggcgggtcgt ggtcatcgcg
    10501 catgtcatct cggccctgct cgccgccatg gccgggatgc tcgccgtggc ccggctgcag
    10561 ctcggccagc cgacgatcgg cgacgactgg ctgatcctct ccttcgccgc cccggtgatc
    10621 ggcggtgccg tgctggccgg cgggcacatc tcggtcatcg gcacctgcct cggcgtggtc
    10681 atcgtcacgc tgatcaacaa tgcgctggtg ctgctcggca tcgatccctt cttcgtccag
    10741 ctcttcctcg gcgggctgat cctgatcgcg gtcgggctca accgctggcg cgagcggcga
    10801 ctggcgccgg catgacggag ccggttctcg cggtcgaggg ggcgaccaag agctttcccg
    10861 gcgtcaaggc gctggacggc gtcgacttcg tcct
//
LOCUS       DAOTFI010000170        10853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7199_length_10853_cov_7.306446, whole genome shotgun sequence.
ACCESSION   DAOTFI010000170 DAOTFI010000000
VERSION     DAOTFI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_10853_cov_7.306446"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="P5558_16315"
     CDS             complement(<1..1161)
                     /locus_tag="P5558_16315"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HRY25934.1"
                     /translation="MSPRLGRRIIAPTAALMATSLVVLLGLMAINAGTEDETARRQSI
                     NTVEQTLHLRETQVGRVAKDYAWWNDTVRYLHVDIDPEWADLNFAGYIFETHNYQYTL
                     VFDGSDQTTYAAFEGERADAGELPSITGGLEQLIVTARHASEREPDYQTGLVLVGGEV
                     AIAAASAITPEETGDFVLPDGPRSVLVFVKLLDEAMLGDIAERFRLTGMRIADVADDG
                     AAASLPLTTVDGAEAGRLAWEPHRPGRQFIRSIAPSLVLALVVIGGFSGVVMRQAGQA
                     AQAFEDSQARFRDVADASSDWIWETDQTLHLAFVSERVAEIIGIPASSMTGRPLDQLF
                     HQGDNEAQWAHYGRALAERLPFRNILAVCDDADGVRHSLRIAMPADTNPHATV"
     gene            1355..2236
                     /gene="speE"
                     /locus_tag="P5558_16320"
     CDS             1355..2236
                     /gene="speE"
                     /locus_tag="P5558_16320"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRY25935.1"
                     /translation="MSWVREAYYPSWQQSLRMDREVFRQQSPHQEILIFDNADLGRVL
                     MLDGLVQATERDEFIYHESLVNLAWASHPAARRALVIGGGDGGCLRELVRHADLEAID
                     LVEIDAAVIDASRRHLPSIHRGAFDDRRVRTVIDDGAAFLERSGETYDLVLVDAPDPV
                     GAGAALFTETFYRACRRCLGAAGVLAAQFGNPLLDAAGQERCAGRLAMAFPAVAFALA
                     PVATYLGGALAIALAGEQPPVRRRDPAHALRWYDAERHDGMRLLGAALDPVPGSIPSL
                     PGRPAGPAHALPAMQTH"
     gene            complement(2248..3105)
                     /locus_tag="P5558_16325"
     CDS             complement(2248..3105)
                     /locus_tag="P5558_16325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRY25936.1"
                     /translation="MLLLLPARLLFVVMPFLALLGILGVPLDGRSETPDVATDEAVID
                     EVDEEAEEEEGESTWLYGEVGVVSDYVDRGISNSDERPALQAGLTYEFDLGLDQEATG
                     YLDLWGSNVDFDDGGEARVELDFSAGLAAPLGESGFDVDVAATYIVYPGADSDLDYDY
                     VEFPLALGYAVTDALSLGLGYIFSPDHSGDVGRAHYVEALAAWTLPWPGDDWPVTLDA
                     RLGRQWFESNSMAGLDDYLDWGIGLTTTVGPVDLGLHYTDTNLGRGDCYGGSDACGAR
                     LVLSAVARF"
     gene            complement(3213..5009)
                     /locus_tag="P5558_16330"
     CDS             complement(3213..5009)
                     /locus_tag="P5558_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HRY25937.1"
                     /translation="MPTFRTENNLLAMFEAQAAAEGDSPFLWQKRDGVYQPWSWRRVA
                     EEAGLLARAFRQLGLEPGDRVVLVAENRPEWCIADLAILGAGGITVPAYTTNTERDHA
                     YILGHSEARMVVCAGRSLAKRLIPAIKESPTVKALLFIEPLAEFGELPVSAMSWADAL
                     ALGERSPAVPGSAAIQPDDVACFIYTSGTGGTPKGVMQTHRNIQANLEGAYELLSQLG
                     IGDREIFLSFLPLSHSYEHTAGQFFPISLGAEIYYAEGVETLSTNLVEARPTILTCVP
                     RLYEVLRQKILVGVNRQGGLQKWLFEQAIQLGTRRYEQGGRLGPLDGLFDALLERLVR
                     TKVNERFGGRLKAMISGGAPLNYDVGLFFAALGLPVLQGYGQTEAAPVISVNVPGRAR
                     HDTVGPPVRGVELRFADDGEIQVRGANVMKGYWKDEEATARTVVDGWLHTGDVGVLDE
                     HGYLKITDRKKDIIVNSGGDNISPQRVEGILLLEPEIGQALIYGDRKPYLVALVTPDP
                     GFVQTFGREAGKSRDPAALAQDPEFRQRIGEAVKRANANLSAIERIRKYEIMAEPFSV
                     ENGMMTPTMKLRRPRIIERHGALLEGLYAAGK"
     gene            5159..7024
                     /locus_tag="P5558_16335"
     CDS             5159..7024
                     /locus_tag="P5558_16335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRY25938.1"
                     /translation="MTKDQRFQAAADMARAAAAEAIAPRWDLGDLFAGFDDPAIEATL
                     RSAAGEAKAVAEAHAGRLGELDGQGLASLIERYEVVQEQLGRVYAFASLSHAGNRDDA
                     LIGRFFQGVQERVNAINTDLLFVTLELNRLDDDALEARIEASPALARFRPWLANVRAF
                     RPHQLSDEIEKILHERSVTGRSAWVRLFDESMAALRFPFRGEELTSAAIFDKLSAKDR
                     TVRRDAATAISGVLEKNIRLCARITNTLVKDKAIEDGWRHFARPISSRNLANQVEDEV
                     VDALISAVRDAYPRLSHRYYALKARWMGLENLEFWDRNAPLPEDSDQRRSWGDAKLIV
                     LDAYRSFSPRLADIVDRFFEKSWIDAEPRPGKDSGAFCHPAVPSVHPYVLMNFQGRAR
                     DVMTLAHELGHGVHQTLAADQGLLMSGTPLTLAETASVFAEQLTFRALLAQAKSPVER
                     RVLLAGKVEDMLNTVVRQIAFCEFERRVHAARAKAELTPDELGDIWMDVQKESLGPAF
                     TYPDEYRAYWSYIPHFVHVPFYVYAYAFGDCLVNALYALYDKAPEGFEPRYVEMLRAG
                     GTLRHKELLAPFGLDATDPAFWNGGLAIIEGFIAELEQELDAGPPDGGKPTGETA"
     gene            7069..8400
                     /locus_tag="P5558_16340"
     CDS             7069..8400
                     /locus_tag="P5558_16340"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRY25939.1"
                     /translation="MGGRFRRYAQVSGAMAGQAARLAGRRLAGTTSSSADRLEHASEL
                     RAALGGLKGPLMKVAQMMATIPEALPAEYAAELAQLQSNAPPMGKPFVRRRMRAELGE
                     DWAKHFRDFPLEPAAAASLGQVHKVVLADGRTAACKLQYPSIASAVDADLSQLRLIFA
                     LYRRYDKAINPDRIYEEIAARLHEELDYVREARNMAVYAEILEGEPHVHVPEVIRELS
                     TGRLLTMSWLEGVRLLDFRTADTATRNRLALNMFRAWYVPFYEFGVIHGDPHLGNYTV
                     RPDLDINLLDFGCVRIFPPRFVKGVIDLFWALMRNDRDLAVAAYECWGFSNLTNEVVD
                     VLNEWAVFLYGPLMEDRARPIHETSSGHQGREVAGKVHQRLREVGGVTPPREFVFMDR
                     AAIGLGSVFLHLHAEINWHQMFLGLIEDFDVDVLAKRQRKVLAAHGLEQPD"
     gene            complement(8542..8748)
                     /gene="rpsU"
                     /locus_tag="P5558_16345"
     CDS             complement(8542..8748)
                     /gene="rpsU"
                     /locus_tag="P5558_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRY25940.1"
                     /translation="MVEVQVRDNNVDQALRALKKKMQREGLYREMRMRRHYEKPSERR
                     AREKAEAIRRYRKLMRKRAERDEV"
     gene            8945..9523
                     /gene="def"
                     /locus_tag="P5558_16350"
     CDS             8945..9523
                     /gene="def"
                     /locus_tag="P5558_16350"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRY25941.1"
                     /translation="MTLRKIALLGHPVLLEPARPVADPEDAEVRALVRDMTETLADSR
                     GLGLAAPQVYNGLRVLLALEPGGDEVDRLAEPLVLINPVLTPLDDRREAAFEGCLSIP
                     GLRGLVPRHHSVGLAALDAEGRPVEREAKGLFARILQHEVDHLDGVLYLDRLTSPRHL
                     AFDSELARLSAYLDEHAVAGRPLAESRPRSDS"
     gene            9520..10251
                     /locus_tag="P5558_16355"
     CDS             9520..10251
                     /locus_tag="P5558_16355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HRY25942.1"
                     /translation="MTEPARDEPTGGKPASDAIDDDAVAGRVAEKDAILEAALGHAAF
                     DGWSRRTLLQAASEVGIDKATARRLFPQGGDSLLAWLDDWADRKMLEAAEPAGLATLP
                     VRQRIATLIRCRLEVLGEHKEALRRAAVARGTPRNLVGGGRAFWRTLDRIWEAAGFGA
                     NGGSRGFSYYSRRTTLGAVLTSTFLYWLEDHSTGHAETWAFLDRRIENVMQFGKLTGR
                     LQGFLDNLPGATFLRRAQRHRTGTR"
     gene            complement(10259..>10853)
                     /gene="flgH"
                     /locus_tag="P5558_16360"
     CDS             complement(10259..>10853)
                     /gene="flgH"
                     /locus_tag="P5558_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRY25943.1"
                     /translation="PMPPPEENRSAGANSLWREGASGFFRDQRARRVGDLMTVAVVID
                     DRAELSSESTRSRANSDQLGIPNFFGYESKLDKIFPSAVDPSALVGATSDMSNQGQGG
                     TNRQETIEFNIAAIVTQVLPNGNMVIDGKQEIRVNFEVREISIAGIVRPEDITPQNTI
                     SQDKIAELRVGYGGRGQITDVQQPRYGAQVLDILLPY"
BASE COUNT         1649 a         3786 c         3686 g         1732 t
ORIGIN      
        1 gacggttgcg tgcgggtttg tgtcggcggg catggcgatg cgcagcgaat ggcgaacccc
       61 gtcggcatcg tcgcagaccg ccaggatgtt gcggaacggc agccgctcgg ccagggcccg
      121 accgtagtgc gcccactggg cctcgttgtc gccctgatgg aagagctggt cgagcggtcg
      181 gccggtcatg ctggaggcgg ggatgccgat gatctcggcg acccgctcgg agacgaaggc
      241 cagatggagc gtctggtcgg tttcccagat ccagtcggag ctggcatcgg cgacatcgcg
      301 gaaccgcgcc tggctgtcct cgaaggcctg tgccgcctgg ccggcctgac gcatcacgac
      361 gccgctgaag ccgccgatca cgacgagggc gagaaccagc gacggggcga tcgagcgaat
      421 gaactggcgg cccggccggt gcggctccca ggccagccgg ccggcctcgg cgccatcgac
      481 cgtggtgagc ggcagggatg ccgcggcgcc gtcgtcggcg acgtcggcga tgcgcatgcc
      541 ggtcaggcga aagcgctcgg cgatgtcgcc gagcatcgcc tcgtcgagca gcttgacgaa
      601 gacgagcacg ctgcgcggcc cgtccggcag cacgaaatcg ccggtctcct cgggcgtgat
      661 ggcgctcgcc gcggcgatcg ccacctcgcc gccgaccagc acgaggccgg tctggtagtc
      721 cggctcgcgc tcgctggcgt ggcgggcggt gacgatcagc tgctcgagcc caccggtgat
      781 cgacggcagc tcgcctgcgt cggcgcgctc accctcgaag gccgcgtagg tcgtctggtc
      841 gctgccgtcg aagaccaggg tgtactggta gttgtgggtt tcgaagatgt agccggcgaa
      901 gttcaggtcc gcccattcgg gatcgatgtc gacgtgcaga taacgcaccg tgtcgttcca
      961 ccaggcgtag tccttggcga cccggccgac ctgcgtctcg cgcaagtgga gcgtctgctc
     1021 gaccgtgttg atcgactggc gccgggcggt ctcgtcctcg gtgccggcat tgatcgccat
     1081 caggccgagc agcacgacga ggctggtggc catcaaagca gcggtcgggg cgatgattcg
     1141 ccgaccgagg cgagggctca tcttttccat ggcgattgca cgtcctgagg cgctgtttcg
     1201 tgcagattgg cccgagtatc gcagccgcgc atgaagacga ggttaacgtc gcggacttga
     1261 ggcgaatccc gccgaccgac cctctccagc agcttgccgc acgccgaaaa agcgatcata
     1321 gttatgatta ttctactttc aggggaatgc ggcgatgagc tgggtccgcg aggcgtacta
     1381 tccgagctgg cagcagagcc tgaggatgga ccgcgaggtg ttccgccagc agagcccgca
     1441 ccaggagatc ctgatcttcg acaatgccga tctcggccgg gtgctgatgc tcgacgggct
     1501 ggtgcaggcg accgagcgtg acgagttcat ctatcacgag agcctcgtca atctcgcctg
     1561 ggcgagccac ccggcggccc gccgcgccct ggtgatcggc ggcggcgatg gcggctgcct
     1621 gcgggagctg gtccggcacg ccgacctcga ggcgatcgat ctggtggaga tcgacgccgc
     1681 ggtgatcgat gccagccggc gccatctgcc ctccattcac cggggcgcct tcgacgaccg
     1741 gcgggtgcgc acggttatcg acgacggtgc cgctttcctg gagcgttccg gggaaaccta
     1801 tgatctcgtc ctggtcgatg cgcccgaccc ggtcggcgcc ggggcggcgc tcttcaccga
     1861 gaccttctac cgggcctgcc ggcggtgcct tggtgctgcc ggcgtgctcg cggcacagtt
     1921 cggcaacccg ctgctcgacg cggccggcca ggagcgctgt gcgggccggc tcgccatggc
     1981 ctttcccgcg gtcgcctttg ccctggcgcc ggtcgccacc tatctcggcg gcgccctcgc
     2041 catcgccctc gccggcgagc agccacccgt tcgccgacgt gaccccgccc atgcgctacg
     2101 ctggtacgac gccgagcgtc atgacgggat gcgcctcctt ggtgcggcgc tcgacccggt
     2161 gccgggcagc ataccctctt tgccgggccg accagccggc ccggcccacg cgctgccggc
     2221 catgcagacc cactaacggt caagtggtca gaagcgggcc accgcactca gcacgagacg
     2281 cgcgccgcag gcgtcgctgc cgccataaca gtcgccgcgg ccgagattgg tgtcggtgta
     2341 gtggaggccc agatcgacgg gcccgaccgt cgtcgtcagc ccgatccccc agtcgagata
     2401 gtcgtcgagg ccagccatgc tgttgctttc gaaccattgt cggccgagcc tcgcgtcgag
     2461 tgtgacgggc cagtcgtcgc cgggccaggg cagggtccag gcggcgagcg cctcgacata
     2521 gtgggcgcgg ccgacatcgc ccgaatggtc cggcgaaaag atgtagccga ggccaaggga
     2581 gagggcgtcg gtgacggcgt agccgagggc caagggaaac tcgacatagt cgtagtcgag
     2641 gtcgctgtcg gcgcccggat agacgatata ggtggcggcg acgtcgacat cgaatccgct
     2701 ctcgccgagc ggtgcggcga gcccggccga gaagtcgagc tcgacccggg cctcgccgcc
     2761 gtcgtcgaaa tccacgttcg agccccagag gtcgagatag ccggtggcct cctggtcgag
     2821 cccgagatcg aactcgtagg tgaggccggc ctgcagtgcc ggccgctcgt cgctgttgct
     2881 gatgccgcgg tcgacatagt cggaaacgac gccgacctcg ccatagagcc aggtcgactc
     2941 gccctcctcc tcctccgcct cttcgtccac ctcgtcgatc accgcttcgt cggtcgccac
     3001 atcgggcgtc tcgctccggc catccagggg cacgcccagg atgccgagca gcgccaggaa
     3061 cggcatcacg acaaagagca gcctggccgg gaggagcagc agcatgacgg acagtccact
     3121 tggcagcagc gtggacgctc gttgtcgccg acggatgttg agatagggtt aacgcggccc
     3181 aaaccatgcg ccggggccgc gcaacggcag tcctacttgc cggcggcgta gagaccctcg
     3241 agcagcgcgc cgtgccgctc gatgatgcgc ggccggcgca gcttcatggt cggggtcatc
     3301 atgccgttct cgacgctgaa cggctcggcc atgatctcgt acttgcggat ccgctcgatc
     3361 gccgagagat tggcgttggc ccgcttgacc gcctcgccga tccgctgccg gaattccggg
     3421 tcctgtgcca gtgccgccgg atccctggac ttgccggcct cccgcccgaa ggtctggacg
     3481 aagcccgggt ccggcgtgac cagcgccacc agatagggct tgcggtcgcc gtagatcagc
     3541 gcctggccga tttccggctc gagcagcagg atgccctcga cacgttgcgg cgagatgttg
     3601 tcgccgcccg agttgacgat gatgtccttc ttgcgatcgg tgatcttgag gtagccgtgc
     3661 tcgtcgagca cgccgacatc gcccgtgtgc agccagccgt cgacgacggt ccgggcggtc
     3721 gcttcctcgt ccttccagta gcccttcatg acgttcgcac cgcggacctg gatctcgccg
     3781 tcatcggcga agcggagctc gacgccgcgg accggtggac cgacggtgtc gtgcctggcc
     3841 cggcccggca cgttgacgct gatcaccggc gccgcctcgg tctggccata gccctgcagc
     3901 accggcaggc cgagggccgc gaagaagagt ccgacgtcgt agttgagcgg cgcacccccg
     3961 gagatcatcg ccttgagccg gccgccgaag cgctcgttga ccttggtccg gaccagccgc
     4021 tcgagcagcg cgtcgaacag gccgtctagc ggacccagcc gaccgccctg ctcatagcgc
     4081 ctggtgccga gttggatcgc ctgctcgaac agccacttct gcagcccgcc ctgccggttg
     4141 acgccgacca ggatcttctg ccgcaacacc tcgtagagcc gcggcacgca ggtcaggatg
     4201 gtgggccgcg cctcgacgag gttggtcgac agcgtctcga ccccctcggc gtagtagatc
     4261 tccgcaccca gcgagatcgg gaagaactgc cctgccgtgt gctcgtagga atgcgacaag
     4321 ggcaggaagg agaggaagat ctcacggtcg ccgatgccga gctggctgag gagctcgtag
     4381 gcgccctcga gattggcctg gatgttgcgg tgggtctgca tgaccccctt gggcgtcccg
     4441 cccgtgcccg aggtgtagat gaagcaggcg acatcgtcgg gctggatggc tgccgagccc
     4501 ggcacggcgg gcgagcgctc gcccagtgcc agcgcgtcgg cccagctcat ggccgagacc
     4561 ggcagctcgc cgaattcggc gagcggctcg atgaagagca gcgccttcac cgtgggtgat
     4621 tccttgattg ccgggatcag ccgcttggcc aggctgcgcc cggcgcagac caccatgcgc
     4681 gcctcggaat ggccgaggat gtaggcgtgg tcccgctcgg tattggtcgt gtaggccggc
     4741 acggtgatgc cgccggcacc caggatcgcg agatcagcga tgcaccattc cggccgattc
     4801 tcggccacca gcacgacccg gtcgccgggc tcgagcccga gctggcgaaa agcccgggcc
     4861 aggagccccg cctcttcagc cacccggcgc cacgaccacg gctgatagac cccgtcacgc
     4921 ttctgccaga gaaaagggct gtcgccctcc gccgccgcct gggcctcgaa catcgcgagc
     4981 aggttgtttt cggtcctgaa ggtcggcatc gcttccctgt gcttcatcct gattcggcaa
     5041 tggtttcctg gcgattcggg ccggcgcgcc gccaccgccg ccgcgaccct tggtcggctc
     5101 gctggacctc caccccctgc cacatatatc tggcatgtcc aggttgaagg gaagttcgat
     5161 gacgaaggac cagcggttcc aggcggccgc ggacatggcc agggccgcgg cggcggaggc
     5221 gatcgccccg cgctgggacc tcggggacct gttcgccggc ttcgacgatc cggcgatcga
     5281 ggcgaccctt cgatcggccg ccggcgaggc gaaagccgtc gccgaggcgc acgccggcag
     5341 gctcggggag ctggatggcc aggggctcgc cagcctgatc gagcgctacg aggtggtcca
     5401 ggagcagctc gggcgggtct acgccttcgc gtcgctcagc catgccggca atcgcgacga
     5461 cgccctgatc ggccgcttct tccagggcgt gcaggagcgg gtcaacgcga tcaacaccga
     5521 cctcctcttc gtcaccctcg agctcaatcg cctcgacgac gacgccctcg aagcgaggat
     5581 cgaggcctct ccggcgctcg cccgctttcg gccatggctc gccaatgtgc gcgcctttcg
     5641 cccgcaccag ctctcggacg agatcgagaa gatcctgcac gagcgatcgg tcaccggccg
     5701 cagcgcctgg gtgcgcctct tcgacgagag catggcggca ctgcgcttcc cgttccgggg
     5761 cgaggagctg accagtgccg cgatcttcga caagctctcg gccaaggacc gcaccgtccg
     5821 ccgcgacgcc gcgaccgcca tcagcggcgt gctcgagaag aacatccgcc tctgtgcccg
     5881 gatcaccaac acgctggtca aggacaaggc gatcgaggac ggatggcgcc atttcgcccg
     5941 gccgatctct tcgcgaaacc tcgccaacca ggtcgaggac gaggtggtgg atgccctgat
     6001 ctcggccgtg cgcgacgcct atccccgcct ctcgcaccgc tactacgcgc tcaaggcccg
     6061 ctggatgggc ctcgagaatc tcgagttctg ggaccgcaac gcgcccttgc cggaagacag
     6121 cgaccagcga aggagctggg gcgacgcgaa gctgatcgtg ctcgacgcct atcgcagctt
     6181 ctcgccgcgc ctcgccgaca tcgtcgatcg cttcttcgag aagagctgga tcgacgccga
     6241 gccgcgtccc ggcaaggaca gtggtgcctt ttgccatccg gccgtgccga gcgtgcatcc
     6301 ctacgtgctc atgaacttcc agggccgggc gcgcgacgtc atgaccctgg cgcacgagct
     6361 gggccatggc gtccaccaga ccctggcggc cgaccagggc ctgctcatgt ccggcacgcc
     6421 cctgacgctc gccgagaccg cctcggtctt cgccgagcag ctcaccttcc gggccctcct
     6481 cgctcaggct aagagcccgg tcgagcgccg ggtgctgctc gccggcaagg tcgaggacat
     6541 gctgaacacc gtcgttcgcc agatcgcctt ctgcgagttc gagcggcggg tgcacgccgc
     6601 gcgggcgaag gccgagctga cgccggacga gctgggcgac atctggatgg acgtgcagaa
     6661 ggagagcctc gggccggcct tcacctatcc ggacgagtac cgggcctact ggagctacat
     6721 cccgcacttc gtccacgtgc cgttctacgt ctacgcctat gccttcggcg actgcctggt
     6781 gaacgccctc tacgccctct acgacaaggc gcccgagggc ttcgagcctc gctatgtgga
     6841 gatgctccgc gccggcggca cgctccgcca caaggagctc ctcgccccct tcggcctcga
     6901 tgccaccgac cccgccttct ggaacggcgg gctcgccatc atcgagggct tcatcgccga
     6961 gctcgagcag gagctggacg caggccctcc ggatggtggc aagccgaccg gagagactgc
     7021 ttgagcgagc gtccgaccgg caccggccgg cgcaacagcg aaagctccat gggcgggcgg
     7081 tttcgccgct acgcccaggt ctccggcgcg atggccggcc aggcggcgag gctcgccggg
     7141 cgtcgcctcg ccggcaccac cagcagcagc gccgaccggc tggagcatgc gtccgagctg
     7201 cgcgcggcgc tcggcgggct caagggcccg ctcatgaagg tggcgcagat gatggccacc
     7261 atcccggaag ccctgccggc cgagtacgcg gccgagctcg cccagctgca gagcaacgcg
     7321 ccgcccatgg gcaagccctt cgtccgccgc cggatgcggg cggagctggg cgaggactgg
     7381 gcgaagcatt ttcgagactt cccgctggag cccgcggccg ccgcctcgct cggccaggtg
     7441 cacaaggtcg tgctggccga cgggcggacc gccgcctgca agctgcaata cccgagcatc
     7501 gcttctgccg tggacgccga tctcagccag ctccggctga tcttcgccct ctatcgccgc
     7561 tacgacaagg cgatcaaccc ggaccgcatc tacgaggaga tcgccgcgcg cctgcacgag
     7621 gagctcgact atgtccgcga ggcgcggaac atggcggtct atgccgagat cctcgaaggc
     7681 gagccgcatg tccacgtgcc cgaggtgatc cgcgagctct cgaccggccg gctgctcacc
     7741 atgtcctggc tcgagggcgt gcgcctgctc gacttcagga ccgccgatac cgccacccgc
     7801 aaccgcctgg cgctcaacat gttccgcgcc tggtacgtgc cgttctacga gttcggcgtg
     7861 atccatggcg acccgcatct cggcaactac acggtgcggc cggatctcga catcaacctc
     7921 ctcgacttcg gctgtgtccg catcttcccg ccgcgcttcg tcaaaggggt gatcgacctc
     7981 ttctgggcgc tgatgcgcaa cgaccgcgac ctcgccgtcg cggcctacga atgctggggc
     8041 ttcagcaacc tgaccaacga ggtcgtcgac gtcctcaacg agtgggccgt cttcctctac
     8101 ggtccgctga tggaggaccg cgcccggccg atccacgaga ccagcagcgg ccaccagggc
     8161 cgcgaggtgg ccggcaaggt gcaccagcgg ctgcgcgagg tcggcggcgt caccccgccg
     8221 cgcgagttcg tcttcatgga ccgcgccgcc atcggcctcg gctcggtctt cctccacctc
     8281 catgccgaga tcaactggca tcagatgttt ctcgggctga tcgaggattt cgacgtcgac
     8341 gttctggcga agcggcagag gaaggtgctg gccgcgcatg ggctcgagca gcccgactga
     8401 tctgcgccgg tcgatgcacg ccgggccggg gcgcaggcgg cgcatagcct cgacctgtcg
     8461 gggggattgc ccaggaatcg aaaagggcgc ccgaccggac gcccttgtca attcccgaag
     8521 tgggagcgag ccgcttgcgg ctcacacctc gtcgcgttcg gcccgcttgc gcatcagctt
     8581 gcggtagcgg cggatcgcct cggccttctc gcgggcgcgg cgctcggacg gcttctcgta
     8641 atgccgccgc atgcgcattt cccgatacag gccctcgcgc tgcatctttt tcttcagcgc
     8701 gcggagcgcc tgatcgacat tgttgtcacg cacctgaact tcgacgatga ccgttctccc
     8761 gtgctggctc gccttgcctc cggtcccgtt cggcagggcg acaaaagggg ttccctcccc
     8821 agagggcgga gccggtggat aacccagatc ggccggccgg gcaagggggt gccgcagcgc
     8881 gccgggacag ggcatttcgc cctgacaccc ttttcgccgg ccccggcacc cttacctatt
     8941 ccgcatgacg ctgcgcaaga tcgccctcct cggccacccc gtcctgctcg agcccgcccg
     9001 cccggtggcg gacccggaag atgccgaagt gcgggcgctg gtccgtgaca tgaccgagac
     9061 cctggccgat tcgagggggc tcggccttgc cgccccgcag gtctacaatg ggctccgggt
     9121 gctcctggca ctggagcccg gcggggacga ggtcgaccgc ctggcggagc cgctcgtcct
     9181 gatcaacccg gtgctgacgc cgctcgacga ccgccgagag gcggccttcg agggctgcct
     9241 ctcgatcccg ggcctgcgcg gcctcgtgcc gcgccatcac agcgtcggcc tcgccgccct
     9301 cgacgcagag gggcggccgg tcgagcgcga ggccaagggg ctcttcgccc gcatcctgca
     9361 gcacgaggtc gatcacctgg acggggtcct ttatctcgat cgcctgacgt cgccccgcca
     9421 tctcgccttc gacagcgagc tggcgcggct gagcgcctat ctcgacgaac atgcggtggc
     9481 gggtcggccg ctggccgaat cccgcccgag gagtgattca tgaccgagcc cgccagagac
     9541 gagccgacgg gcggcaagcc agcgagcgac gcgattgacg acgacgccgt cgccggccgg
     9601 gtggccgaga aggacgcaat cctcgaggcg gcgctcggcc atgccgcctt cgacggctgg
     9661 agccgccgca cgctgctcca ggcggcgagc gaagtcggga tcgacaaggc aacggcgcgc
     9721 cgcctctttc cccaaggcgg cgacagcctg ctcgcctggc tcgacgactg ggccgaccgg
     9781 aagatgctcg aggccgccga acctgcggga ctcgccaccc tgccggtccg ccagcgcatc
     9841 gccacgctga tccgctgccg cctcgaggtg ctgggcgagc acaaggaagc gctgcggcgg
     9901 gccgccgtgg cgcgcggcac gccccgcaat ctcgtcggcg gcggtcgcgc cttctggcgc
     9961 acgctggacc gcatctggga agccgccggt ttcggggcca acggcgggag ccgcggtttc
    10021 agctactaca gccgaaggac aacgctcggc gcggtcctca cctcgacctt cctctactgg
    10081 ctcgaggacc attcgacggg ccatgccgag acctgggcct tcctcgaccg gcggatcgag
    10141 aacgtgatgc agttcggcaa gctcacgggc aggctccagg gcttcctcga caacctgccg
    10201 ggggccactt tcctgcgccg ggcgcagcgc caccggacgg gcacccggta gaggccggtc
    10261 agtagggcag gaggatgtcg agcacctggg cgccgtagcg cggctgctgg acgtcggtga
    10321 tctggccacg gccgccatag ccgaccctaa gctcggcgat cttgtcctgg ctgatggtgt
    10381 tctgcggcgt gatgtcctcg ggccggacga tgccggcgat cgagatctcc cggacctcga
    10441 agttcacccg gatctcctgc ttgccgtcga tcaccatgtt gccgttgggc aggacctggg
    10501 tgacgatcgc ggcgatgttg aactcgatcg tctcctggcg gttggtgccg ccctggccct
    10561 ggttcgacat gtcgctcgtg gcgccgacca gggcgctcgg atcgacagcg ctcgggaaga
    10621 tcttgtcgag cttgctctcg tagccgaaga agttcgggat gccgagctgg tcggagttgg
    10681 cgcggctgcg cgtgctctcg ctcgagagct cggcgcggtc gtcgatgacg acggccacgg
    10741 tcatcaggtc gccgacgcgc cgggcgcgct ggtcgcggaa gaagccgctg gcgccctcgc
    10801 gccagagtga gttggcgccc gcactgcggt tctcctctgg cggcggcatc ggc
//
LOCUS       DAOTFI010000171        10835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7211_length_10835_cov_6.633302, whole genome shotgun sequence.
ACCESSION   DAOTFI010000171 DAOTFI010000000
VERSION     DAOTFI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7211_length_10835_cov_6.633302"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="P5558_16365"
     CDS             complement(<1..704)
                     /locus_tag="P5558_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY25944.1"
                     /translation="MSNVCLITGGARGIGAATARLVAKDGMAVAIGYRERADAAEALA
                     AEIVAAGGRAAPVEGDVAREADIVAMFDAAEKALGPVTGLVNSAGISIKSAVRDFAAA
                     DLERLMAVNVIGLILCCREAARRMPESGGAIVNISSMAATIGGRAGASAYAASKAAVD
                     SFTTGFAKEVGKRRIRVNVVRPGMTRTDMTAGQLGDPKVEAKLAQTIPMHRVGEAIEI
                     AEAVRWLLSPAASFISG"
     gene            complement(725..2317)
                     /locus_tag="P5558_16370"
     CDS             complement(725..2317)
                     /locus_tag="P5558_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY25945.1"
                     /translation="MAGASESVFDVIVVGAGSAGCVLANRLSADPKRRVLLLEAGGRD
                     WNPWIHIPGGFFKTIWHKDLSWNFETEDEPGLKGRSQVWPRGRVLGGSSSLNAMLYVR
                     GQAADFEMWRQLGNTGWSYADVLPYFRRAEDQERGADDYHGAGGPLAVSNARAPHSLS
                     DAFVEAAVEAGFPRSDDFNGAVQEGVGYYQLTARNGRRCSTARGYLVPAKGRPNLEVR
                     TRAEVHRLLVENGRAAGVVLADGAIRARHVVLSAGAIKSPQILMLSGIGKAEDLRKLG
                     ITPVVDLPGVGDNLQDHLQVKLIFKVHGVETLNEVMRNPLLQAREAARYALFRTGALA
                     AGPSFCGGFVRSDPALDLPDLQLHFNPLSGDRPAHLHDFPGCSPIVSQLRPQSRGRLW
                     LKSPVPTDPPAMVANYLDDETDRRVVVEGMRLVRKIMAQPAMAKFRPEAYLPDEGPQS
                     DAELLDVARRVGSTQYHPISTCRMGTGPDAVVDPELRVRGIEGLTVADASIMPTMVSG
                     NTNATCIMIGEKAADLVAARLR"
     assembly_gap    2391..2490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2491..3614)
                     /locus_tag="P5558_16375"
     CDS             complement(<2491..3614)
                     /locus_tag="P5558_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY25946.1"
                     /translation="MTTKGEAAGQRPNIILILTDQQRFDTINALGASWVETPTLDRLI
                     NEGQVFTDCHITAASCVPCRASLFKGYYPHTTGVLRNGQPWARTWVEQLADAGYRCVN
                     VGKMHTIPYNAPAGFHERYVVENKDRYMEGRWYFDEWDKALAAHGLKKQQRVEYRKRD
                     DYRERLGAFEWELPEHLHSDVFVGNMAKWWVDTYPQTEPLFLQVGFPGPHPPYDPTSR
                     YAEPYLKRNDIPIPRPTRAELDSLVPPFKEKRQHDVEVDHDSVAWSLNPTDEQLHRLW
                     AYYGANVTMIDDSIEALLKSLEARGYLENAVVFFASDHGDCMGDHGMIQKWSMYDCVT
                     RTPLIAWSPGRFEAGRKVDGLCQLFDLGPTILELAGCAVP"
     gene            complement(3611..5101)
                     /locus_tag="P5558_16380"
     CDS             complement(3611..5101)
                     /locus_tag="P5558_16380"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY25947.1"
                     /translation="MLEAFANLLAFWPLTYLALGVALGIVVGSIPGLTASMLIALSLP
                     LTFYMSSVDAMTLLIGMYVGGISGGLIAATLLRMPGTPASIMTTFDGFPMAQQGKGER
                     ALALGIAASIIGGLISWVFLAVLSPPLANLALRFGPWEYFTMVLMALVMLASLSQGSL
                     VKGLIAACLGMLLAMPGIDPSIGQLRLTFGWDKLTGGLALLPVLIGTFAISQILKDVI
                     AIEQRLAEVKVMRRAVAIKARDLVTHGPNLLRSSVIGTWVGLLPGIGGNIAAMLAYAT
                     AKSVSREPEKFGKGSESGVVAAETANNASIGGALIPLITMGIPGSVVEAILIGALIIH
                     NLQPGPLLFVTNPEIAYGVIASYLVANIFMAIMMLAAVGWISRLMYLPRAYLLTAILF
                     FCVVGTFALNNRFFDVWVMLAFGVVGFALEYCKVPLGPFVIGFVLAPIAEEQLRAGMM
                     ASEGDFLALFGRPFAATFFVVSILTLCWPLYQEWRQRRRASAEVHT"
     gene            complement(5118..5594)
                     /locus_tag="P5558_16385"
     CDS             complement(5118..5594)
                     /locus_tag="P5558_16385"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY25948.1"
                     /translation="MEGPGREPLADILFAVAVLALAGIVWWGTASLPPPRYEPLGSAA
                     LPRTLAVVMALLGLVLLGRAWAGRRQAAPAAVGLGFRPRPWLAVGVLAVLVLYVAAMD
                     LRLLGFVPASILALVLIGAMLTGFDRRRLPWLAGFVVLLVVAVQLLFTRVFYIDLP"
     gene            complement(5632..6588)
                     /locus_tag="P5558_16390"
     CDS             complement(5632..6588)
                     /locus_tag="P5558_16390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY25949.1"
                     /translation="MNKLLLGSLLAGAALALGAVPAPALAFPDKPIRVIVGFAPGGRT
                     DTVARLLAAKIEEKGLLPQPLVIVNMPGGGGAVGAQEVLDAEPDGHTIVHWHHQLLIA
                     NAMEIIEAGPDDFTSIGFTGGGSPVWTVRADLGLETLDDVVQKLKAEPGSLVEVIGIG
                     TIPHFVGALLAKEAGFETRKVQAASGADRLKMIAGGNADISLFAASEYAGWKTAGEGL
                     EAVVFFGPNRIDTIPEVPTAKELGYDVVWANPNWWLAPAGTPQENIDILAAALEEAFQ
                     DEEIKAWFAENSLDHYWTPGPQAHEESVELLGRLQEVAAEIR"
     gene            complement(6585..7682)
                     /locus_tag="P5558_16395"
     CDS             complement(6585..7682)
                     /locus_tag="P5558_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY25950.1"
                     /translation="MADAVPELAPAAPTARRAVRLKEVAARAGVSINTVSRAIRAPAT
                     VRPELRRHIEAVLDELDYVPNRLAGGLASAHTGVVGVVVTSLLYSEFAAMIEGLQGGL
                     ARGGLSTMLANSHYRPEEELRLVRAMLSWRPAAMALIGIDHHPRVSELLRHSGMPVVE
                     LWETGDAVIDSVVGMDHAGIGRAQASHLLGQGYRRIAFTGGVRPHDHRAQRRRDGCVA
                     LLEERGCEPLVVTAAEGGTADLGETLLDRLLERAPDIDGIVCNSDVIAFGVLRGLTRR
                     GRRVPDEIGVIGFGDNEAASCMQPPLSTMRPPRAEIGQAAAAAVLARIEGGPAIRRVF
                     TAELIARSSTARDPAAAGTGAATSSIREKSS"
     gene            7844..9247
                     /locus_tag="P5558_16400"
     CDS             7844..9247
                     /locus_tag="P5558_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25951.1"
                     /translation="MDFDRQSRSSGARATLGAARLHRRQLLQSALAGSAALAVGLRAP
                     AVLGQAKPFAGVTLQGASFASTFFGYLENYIPEFEEATGMKVNFETNAFPVFNQRTDL
                     ELSTQGSALDVINVTFIYSGRWIGAGWVENLKPYLEDPNLTPDDWDPKDFVGGTMASL
                     SDANGDPHGFPWEAGAMILAASRADLLEQKGLSLPTTFDELMNVCDQTKGMERVAPFT
                     ADKLHHWNWIPYLMGFGGTVFKDPPGNLTPTWDTPQAAEAADFYGRLLSEYGPPGVLS
                     FTDDQAMQSQKTGRANMRTQAITWMVPLAKDPDSRVKDTVRYGLVPAGPAGAFPGSNS
                     HGFGIPVGSRNKEAAWEFIKWAVGKQTTLRMLEQHGYPSICRRSIIDTQLFKDTLTLN
                     GQDVAALYLQVLELGGKGGYMKYRTVPQFPQIGDKLNKAIEKVATGQATGLEAMAEAQ
                     AEAVADLKKSGVQIDAG"
     gene            9252..10208
                     /locus_tag="P5558_16405"
     CDS             9252..10208
                     /locus_tag="P5558_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY25952.1"
                     /translation="MQRSGGLTADRATGSTAPAAGPARRFEDQRRRFYSNLLLPALAA
                     LMVVTLLPTIYLVLVSLTPLDLARPETHWDFSRPFAQYENVLNDSRLHNSLWVQAKLS
                     FWTVLLQVVLGFVFALLLNVSSKVLMALRTVFLIPMVLPPIVVAIIWKVLYTPDISPL
                     HWATEAMGFVVPSLITDPSWALTAIIVADVWEWFPFVMLMVLASLQMMPSEYIEAARV
                     DGASVLQLTFYVTLPYLKGVLLVAALFRLVDSIKAFPLIYILTDGGPGTVTEVTNFYS
                     FIQAFNFSYLGFSSAITVVLVVITVFLSWLIIRMVGWGQRVD"
     gene            10198..>10835
                     /locus_tag="P5558_16410"
     CDS             10198..>10835
                     /locus_tag="P5558_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY25953.1"
                     /translation="MSTSTDYQSHLKWQRFWLHALVIVLVLLVMFPFVWLLIMSFKTI
                     DDIFAWPPKWLFVPTLENYTGLWEGDFPKSFANSLIVSVGSTAIAILLGVPGAYALSR
                     MDMGRSNQLSILILASRMAPPIAFTIPYFLAYRHIGLLDSRLGLVIIYLTFNLSLVVW
                     LMRSFFDATPRALEEAAWIDGASLWQSFVKIILPLSGPGLAATAILCFLYSW"
BASE COUNT         1662 a         3793 c         3651 g         1629 t
ORIGIN      
        1 ccgctgatga aggaggcggc gggggagagg agccagcgga cggcctcggc gatctcgatg
       61 gcctcgccga cccggtgcat cgggatggtc tgggcgagct tcgcctccac cttcgggtcg
      121 ccgagctggc cggctgtcat gtcggtgcgg gtcatgccgg ggcggacgac gttgacgcgg
      181 atcctgcgct tgccgacctc cttggcgaag cccgtggtga agctgtcgac ggcggccttc
      241 gacgccgcat aggcgctggc cccggcgcgc ccgccgatgg tcgccgccat ggacgagatg
      301 ttgacgatcg ccccgccgct ctcgggcatg cggcgcgccg cctcgcggca gcagaggatc
      361 aggccgatga cgttgaccgc catcagccgc tcgaggtcgg cggccgcgaa gtcgcggacc
      421 gccgacttga tgctgatgcc ggcgctgttg acgaggccgg tcaccggccc gagcgccttt
      481 tcggcggcgt cgaacatcgc gacgatgtcg gcctcgcggg cgacatcgcc ctcgaccggc
      541 gccgcacggc cgccggcagc aacgatctcg gccgccagcg cctcggccgc atcggcccgc
      601 tcgcgatagc cgatggcgac ggccatgccg tccttggcga ccagccgcgc ggtcgcagca
      661 ccgatgccgc gggcacctcc ggtgatcagg cagacgttcg acacggctct tcctccctct
      721 ttattcagcg caggcgggcg gcgaccaggt ccgccgcctt ctcgccgatc atgatgcagg
      781 tggcgttggt gttgccggag accatggtgg gcatgatcga ggcatcggcg accgtcaggc
      841 cctcgatgcc gcgcaccctg agctccgggt cgaccaccgc gtcggggccc gtgcccatgc
      901 ggcaggtcga gatcgggtgg tactgggtcg agcccacccg gcgggccacg tcgagcagct
      961 cggcgtcgct ctgcggcccc tcgtcgggca ggtacgcctc gggccggaat ttcgccatgg
     1021 ccggctgggc catgatcttg cggaccagcc gcatcccttc gaccaccacc ctccggtcgg
     1081 tctcgtcgtc gagatagttc gcgaccatgg ccggcgggtc ggtcggcacc gggctcttga
     1141 gccagagccg cccccggctc tgcgggcgaa gctgcgagac gatcggcgag cagccgggaa
     1201 agtcgtgcag atgggccggc ctgtcgcccg agagcgggtt gaagtggagc tgcaggtccg
     1261 ggagatcgag ggccgggtcg ctccggacga agccgccgca gaaggacggc cccgccgcca
     1321 gcgccccggt gcgaaagagg gcgtacctcg ccgcctcgcg cgcctgcagc aggggattgc
     1381 gcatcacctc gttcagcgtc tcgaccccgt gcaccttgaa gatcagcttc acctgcagat
     1441 ggtcctggag gttgtcgccg acgccgggca gatcgaccac gggcgtgatg ccgagcttcc
     1501 gcagatcctc cgccttgccg atgccgctca gcatcaggat ctgcggcgac ttgatcgccc
     1561 ccgccgagag cacgacgtgg cgcgcccgga tggcgccatc cgccaggacc acaccggcag
     1621 cccggccgtt ctcgacgagc agccgatgca cctcggcgcg cgtgcggacc tcgaggttgg
     1681 ggcgcccctt ggcgggcacg agatagcccc gggcggtgct gcagcgacgg ccgttcctgg
     1741 ccgtgagctg gtagtagccg accccctcct ggaccgcccc gttgaagtcg tcgctgcgcg
     1801 gaaacccggc ctcgaccgcg gcctcgacga acgcgtccga gaggctgtgc ggggcgcgtg
     1861 cattgctcac ggccagcggc ccgccggcgc cgtgatagtc gtcggcaccc cgctcctggt
     1921 cctcggcccg gcggaaatag ggcagcacgt cggcgtagga ccagcccgtg ttgccgagct
     1981 gccgccacat ctcgaagtcg gccgcctggc cccgcacata gagcatggcg ttgagcgagg
     2041 aggagccgcc gagcacccgc ccgcgcggcc agacctggct cctgcccttc agccccggct
     2101 cgtcctcggt ctcgaagttc cagctcaggt ccttgtgcca gatggtcttg aagaagccgc
     2161 ccgggatgtg gatccagggg ttccagtccc tgccgcccgc ctccagcagc aggacacggc
     2221 gcttcgggtc ggcggagagg cggttggcga gcacgcagcc cgccgagcct gcccccacga
     2281 cgatgacgtc gaacacggat tcgctcgctc ccgccatggc cggcctaccg gtgctccgcc
     2341 atccagtcgc gggtccggta gttgctctcg atcagccagt cgcgcagcgt nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgggcaccg cgcagccggc gagctcgagg
     2521 atggtggggc cgaggtcgaa gagctggcag agcccgtcca ccttccggcc ggcctcgaac
     2581 cggccgggcg accaggcgat cagcggcgtg cgggtgacgc agtcgtacat cgaccacttc
     2641 tggatcatgc cgtggtcgcc catgcagtcg ccatggtccg aggcgaagaa gacgaccgcg
     2701 ttctcgagat agccacgggc ctccagcgac ttcaagagcg cctcgatgga gtcgtcgatc
     2761 atcgtcacgt tggcgccgta ataggcccag agccggtgca attgctcgtc ggtcgggttc
     2821 aggctccagg cgaccgaatc gtggtcgacc tcgacgtcgt gctgccgctt ctccttgaag
     2881 ggcggcacca ggctgtcgag ctcggcccgg gtcggccgcg ggatcgggat gtcgttgcgc
     2941 ttcagatagg gctcggcata gcggctggtc gggtcgtagg gcgggtgcgg cccgggaaag
     3001 cccacctgga ggaagagcgg ctcggtctgg gggtaggtgt cgacccacca tttcgccatg
     3061 ttgccgacga agacgtcgga atggagatgc tccggcagct cccactcgaa ggcgccgagg
     3121 cgctcccgat agtcgtcgcg cttcctgtac tcgacccgct gctgcttctt cagcccgtgc
     3181 gcggcgagcg ccttgtccca ctcgtcgaag taccagcgcc cctccatgta gcggtccttg
     3241 ttctccacca cgtagcgctc gtggaagccg gccggggcgt tgtaggggat ggtgtgcatc
     3301 ttgccgacat tgacgcaacg atagccggca tcggcgagct gctcgaccca ggtccgggcc
     3361 cagggctggc cgttcctcaa gacgcccgtg gtgtgcgggt agtagccctt gaagaggctc
     3421 gcccggcagg ggacgcagga ggcggcggtg atgtggcagt cggtgaagac ctgcccctcg
     3481 ttgatcagcc tgtccagggt cggcgtctcg acccaggagg cgcccagcgc gttgatcgtg
     3541 tcgaaccgct gctggtccgt caggatcagg atgatgttgg gccgctggcc ggccgcctcg
     3601 cctttggtcg tcatgtgtgc acctccgctg aagcacgtcg ccgctggcgc cactcctgat
     3661 agagcggcca gcagagcgtc aggatcgaaa ccacgaagaa ggtcgccgcg aacggccggc
     3721 cgaagagggc gaggaagtcg ccttccgagg ccatcatgcc ggcccgcagc tgctcctcgg
     3781 cgatgggggc gagcacgaag ccgatgacga aggggccgag cggcaccttg cagtattcga
     3841 gcgcgaagcc gaccacgccg aacgccagca tcacccagac gtcgaagaag cggttgttga
     3901 gcgcaaacgt gccgaccacg cagaagaaga ggatcgcggt caaaagatag gcgcgcggca
     3961 ggtacatgag ccgcgagatc cagcccaccg ccgccagcat catgatcgcc atgaagatgt
     4021 tggcgacgag gtaggacgcg atcacgccgt aggcgatctc ggggttggtc acgaagagga
     4081 gcggccccgg ctgcaggttg tggatgatca gggcaccgat caggatcgcc tcgaccacgc
     4141 tgccggggat gcccatggtg atcagcggga tgagcgcccc gccgatcgag gcgttgttgg
     4201 cggtctcggc cgcgaccaca cccgactcgc tgcccttgcc gaacttctcc ggctcccgcg
     4261 agacgctctt cgccgtggcg taggcgagca tggcggcgat gttgccgccg atgccgggca
     4321 ggagcccgac ccaggtgccg atgaccgagg agcggagcag gttcggcccg tgggtgacga
     4381 ggtctctggc cttgatggcg acggcccggc gcatgacctt gacctccgcc agccgctgct
     4441 cgatggcgat gacgtccttg aggatctggc tgatggcgaa ggtgccgatc agcaccggca
     4501 gaagcgcgag cccgccggtc agcttgtccc agccgaaggt cagccggagc tggccgatcg
     4561 aggggtcgat gccgggcatg gcgagcagca tgccgaggca ggcggcgatc agccccttga
     4621 ccagcgagcc ctggctcaag gacgccagca tgaccagcgc catcagcacc atggtgaagt
     4681 attcccaggg cccgaaccgc aaggcgaggt tggcgagcgg cggcgagagc acggcgagga
     4741 agacccagga gatcagcccg ccgatgatcg aggcggcgat gccgagggcg agcgcccgct
     4801 cgcccttgcc ctgctgggcc atggggaagc cgtcgaacgt ggtcatgatc gaggccggcg
     4861 tgcccggcat gcgcagaagc gtggcggcga tgagcccgcc ggagatgccg cccacataca
     4921 tgccgatgag cagcgtcatg gcgtcgacgc tcgacatgta gaaggtgagc ggcagcgaga
     4981 gcgcgatcag catcgaggcg gtgaggcccg gaatggagcc cacgacgatg ccgagcgcca
     5041 cgccgagcgc cagataggtg agcggccaga aggcgagaag attggcgaag gcctcgagca
     5101 tcaggtgtgg gccccgctca cggcaggtcg atgtagaaga cgcgggtgaa gaggagctgg
     5161 accgcgacca ccaggagcac gacgaacccc gcgagccagg gcagccgtcg gcggtcgaag
     5221 cccgtgagca tggcgccgat caggacgagc gccaggatcg aggccggcac gaagccgagc
     5281 agccggagat ccatggccgc gacataaagg acgaggacgg cgagcacgcc gaccgcgagc
     5341 cagggccgcg gccggaagcc caggcccacc gccgccggcg ccgcctgccg ccgccccgcc
     5401 caggcacgcc cgagcagcac gaggccgagc agcgccatga cgacggcgag cgtgcgcggc
     5461 agcgcggccg agccgagcgg ctcgtagcgc ggcggcggca gggaagccgt gccccaccag
     5521 acgatgccgg ccagcgccag caccgccacg gcgaagagga tgtcggcgag cggctcccgc
     5581 ccgggccctt ccatcgaacg cgcgcccttc ccccggcggt tccggcccgc ctcagcggat
     5641 ctcggccgcc acctcctgca gccggccgag cagctcgacc gattcctcgt gcgcctgcgg
     5701 ccccggtgtc cagtagtggt cgagcgagtt ctcggcgaac caggccttga tctcctcgtc
     5761 ctggaacgcc tcctcgaggg ccgcggccag gatgtcgatg ttctcctgcg gcgtgccggc
     5821 cggcgccagc caccagttgg ggttggccca gacgacgtcg tagccgagct ccttcgcggt
     5881 cggcacctcg gggatcgtgt cgatccggtt ggggccgaag aagacgaccg cctcgagccc
     5941 ttcgccggcg gtcttccacc cggcatattc cgatgccgcg aagagcgaga tgtcggcatt
     6001 gccgccggcg atcatcttca gccggtcggc gccgctggcc gcctggacct tgcgggtctc
     6061 gaagcccgcc tccttggcga gcagcgctcc gacgaagtgc gggatcgtgc cgatgccgat
     6121 gacctcgacc aggctgccgg gctcggcctt gagcttctgc accacgtcgt cgagcgtctc
     6181 gagcccgaga tcggcgcgca ccgtccagac cgggctgccg ccgccggtga agccgatcga
     6241 ggtgaagtcg tccggcccgg cctcgatgat ctccatcgcg ttggcgatca ggagctggtg
     6301 gtgccagtgc acgatggtgt ggccgtccgg ctccgcgtcg agcacctcct gggcaccgac
     6361 ggcaccaccg ccgcccggca tgttgacgat gacgagcggc tgcggcagca ggcccttctc
     6421 ctcgatcttg gcggcgagca gccgtgccac cgtgtcggtc cggccgcccg gggcgaagcc
     6481 gacgatgacg cggatgggct tgtccggaaa ggccagggcc ggcgcgggca cggcgccgag
     6541 ggccagggcc gctccggcga gcaggctgcc gagcaagagc ttgttcatga cgatttctcc
     6601 ctgatggatg atgtcgcggc gcccgtcccg gccgctgccg ggtcgcgcgc cgtgctggac
     6661 cgggcgatca gctcggcggt gaagacccgg cggatcgccg gcccgccctc gatccgggcg
     6721 agcaccgccg cggcggccgc ctggccgatc tcggcgcgcg gcgggcgcat ggtcgacaag
     6781 ggtggctgca tgcaggaggc ggcctcgttg tcgccgaagc cgatcacgcc gatctcgtcc
     6841 ggaacccgcc gcccgcgccg ggtcaggccg cgcagcacgc cgaaggcgat cacgtcgctg
     6901 ttgcagacga tgccgtcgat gtcgggcgct ctctccagca gccggtcgag cagcgtctcg
     6961 ccgagatcgg ccgtgccgcc ttccgcggcg gtcaccacga gcggctcgca gccgcgctcc
     7021 tccaggaggg cgacacagcc gtcccggcgg cgctgtgcgc ggtggtcgtg cggtcgcacg
     7081 ccgcccgtga aggcgatgcg ccgatagccc tgcccgagca gatggctggc ctgggcgcga
     7141 ccgatccccg cgtggtccat gccgacgacc gaatcgatca cggcatcgcc ggtctcccag
     7201 agctcgacca ccggcatgcc ggaatggcgc aggagctcgg agacacgggg gtggtgatcg
     7261 atgccgatca gcgccatggc cgccggccgc cagctcagca tcgcccgcac cagccgcagc
     7321 tcctcctccg gccggtagtg gctgttggcc agcatcgtgc tcagcccgcc gcgcgcgagg
     7381 ccgccctgca ggccctcgat catcgccgcg aactcggaat agaggagcga ggtgacgacc
     7441 accccgacca cgccggtatg ggcgctggcg agcccgccgg cgagccggtt gggcacgtag
     7501 tccagctcgt cgagcaccgc ctcgatgtgc cggcgcagct ccgggcggac cgtcgccggg
     7561 gcccggatcg cccgcgatac cgtgttgatc gagacgcccg cccgtgccgc gacctccttg
     7621 agccgcaccg cgcgccgcgc ggttggcgcc gccggagcga gttcggggac ggcgtcagcc
     7681 acgaccaggg ttctcccgcg ttctgcgatg cccgaaaggg gcgtgaaatg agcgttgcgt
     7741 tagcgctcac ttttgttacc ctgcaccagg acaagcggtc gaggcaagcg gaacatcgac
     7801 ccggcgccgg cggaacgcgg cgcgcagcac agggagccgt accatggatt ttgatcgaca
     7861 gagccggtcg agcggcgcac gcgccacgct gggcgccgcc cgcctgcacc ggcgccagct
     7921 cttgcagagc gccctcgccg gctcggcggc gctggccgtc ggcctcaggg ccccggcggt
     7981 gctcggccag gccaagccgt tcgccggcgt gaccctgcag ggtgcgtcct tcgccagcac
     8041 cttcttcggc tatctcgaga actacatccc ggagttcgag gaagcgaccg ggatgaaggt
     8101 caacttcgag accaacgcct tccccgtctt caaccagcgc accgacctcg agctctcgac
     8161 gcagggcagc gccctcgacg tcatcaacgt caccttcatc tattccggcc gctggatcgg
     8221 cgccggctgg gtcgagaacc tcaagcccta tctcgaggac ccgaacctga cccccgacga
     8281 ctgggatccc aaggacttcg tcggcggcac catggcctcc ctctcggacg ccaacggcga
     8341 cccccacggc ttcccctggg aagccggggc gatgatcctg gcggcgtcca gggccgatct
     8401 gctcgagcag aagggcctct cgctgccgac caccttcgac gagctgatga acgtctgcga
     8461 ccagacgaag ggcatggagc gggtcgcccc cttcaccgcc gacaagctgc accactggaa
     8521 ctggatcccg tacctgatgg gcttcggcgg cacggtcttc aaggacccgc ccggcaacct
     8581 gacgcccacc tgggacacgc cccaggccgc cgaggccgcc gacttctacg gccggctgct
     8641 cagcgagtac ggcccgcccg gcgtgctgtc gttcaccgac gaccaggcca tgcagtcgca
     8701 gaagaccggc cgcgccaaca tgcgcaccca ggcgatcacc tggatggtgc cgctcgccaa
     8761 ggacccggac agccgcgtca aggacacggt ccgctacggg ctggtgcctg ccggcccggc
     8821 cggcgccttc ccgggctcca acagccacgg cttcggcatc ccggtgggct cgaggaacaa
     8881 ggaggccgcc tgggagttca tcaagtgggc ggtcggcaag cagacgacgc tcaggatgct
     8941 cgagcagcac ggctatccga gcatctgccg ccgctcgatc atcgacaccc agctcttcaa
     9001 ggacacgctg accctgaacg gccaggatgt cgcggccctc tacctccagg tgctcgagct
     9061 cggcggcaag ggcggctaca tgaagtaccg caccgtgccg cagttcccgc agatcggcga
     9121 taagctcaac aaggcgatcg agaaggtggc gaccggccag gcgaccggtc tcgaggcgat
     9181 ggccgaggcc caggccgagg cggtcgccga cctgaagaag tccggcgtcc agatcgacgc
     9241 cggctgatcc gatgcagcgg tcgggcgggc tcactgcgga ccgggcaacg gggagcaccg
     9301 cccccgcggc cgggcccgcc cgccgcttcg aggaccagcg ccggcggttc tactccaacc
     9361 tcctgctgcc ggcgctggcc gccctcatgg tcgtcacgct gctgccgacc atctatctcg
     9421 tgctcgtcag cctgaccccg ctcgacctcg ccaggcccga gacccactgg gacttctccc
     9481 gacccttcgc ccagtacgag aacgtgctga acgacagccg gctgcacaac tcgctctggg
     9541 tccaggccaa gctctcgttc tggaccgtgc tgctgcaggt cgtgctcggc ttcgtcttcg
     9601 cgctcctgct caatgtcagc tcgaaggtgc tgatggcgct gcgcaccgtc ttcctcatcc
     9661 cgatggtgct gccgccgatc gtcgtcgcga tcatctggaa ggtgctctac acgccggaca
     9721 tcagccccct gcactgggcg accgaggcca tgggcttcgt cgtcccgtcg ctgatcaccg
     9781 accccagctg ggccctgacc gcgatcatcg tcgccgacgt ctgggagtgg ttccccttcg
     9841 tcatgctgat ggtgctcgcc agcctgcaga tgatgccgtc cgaatacatc gaggcggcca
     9901 gggtggacgg cgcctcggtg ctccagctca ccttctacgt caccctgccc tacctgaagg
     9961 gcgtgctcct ggtcgccgcc ctcttccgcc tggtcgacag catcaaggcc ttcccgctca
    10021 tctacatcct gaccgacggc ggccccggca cggtgaccga ggtcaccaac ttctactcgt
    10081 tcatccaggc cttcaacttc tcctatctcg gcttctcgag cgccatcacc gtggtgctcg
    10141 tcgtcatcac ggtcttcctg agctggctga tcatccgcat ggtgggctgg gggcagcgtg
    10201 tcgactagca ccgactacca gtcccacctc aaatggcagc ggttctggct gcacgcgctg
    10261 gtcatcgtgc tcgtgctgct cgtcatgttc ccgttcgtct ggctcctgat catgtcgttc
    10321 aagaccatcg acgacatctt cgcctggcca ccgaaatggc tcttcgtgcc gacgctcgag
    10381 aactacaccg ggctctggga gggcgacttc cccaagagct tcgccaacag cctgatcgtc
    10441 agcgtcggct cgaccgcgat cgcgatcctg ctcggcgtgc ccggcgccta cgccctctcg
    10501 cgcatggaca tgggccggag caaccagctc agcatcctga tcctggcgag ccgcatggcg
    10561 ccgccgatcg ccttcaccat cccctacttc ctggcctatc gccatatcgg cctgctcgac
    10621 agccggctcg gcctcgtcat catctacctg acgttcaatc tctcgctggt cgtctggctg
    10681 atgcgcagct tcttcgacgc gacgccacgg gccctcgagg aggccgcctg gatcgacggc
    10741 gcctccttgt ggcagtcctt cgtcaagatc atcctgccgc tctccggccc cggcctcgcc
    10801 gcgaccgcga tcctctgctt cctctattcc tggaa
//
LOCUS       DAOTFI010000172        10720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7311_length_10720_cov_8.008533, whole genome shotgun sequence.
ACCESSION   DAOTFI010000172 DAOTFI010000000
VERSION     DAOTFI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7311_length_10720_cov_8.008533"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="P5558_16415"
     CDS             <1..1235
                     /locus_tag="P5558_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRY25954.1"
                     /translation="RLIDYMADEFKKEQGIDLRSDKLALQRLKEAAEKAKIELSSTQQ
                     TEVNLPFITADQSGPKHLTMKISRAKLEMLVDDLIQRTIEPCKKALKDAGLAASDIHE
                     VILVGGMTRMPKVVETVKQLFGKEPHKGVNPDEVVAMGAAIQAGVLAGDVKDVLLLDV
                     TPLSLGIETLGGVFTRIIDRNTTIPTKKSQVFSTADDNQSAVTIRVFQGERELAAQNK
                     LLGQFDLVGIPPAPRGMPQIEVTFDIDANGIVNVSAKDKATGKEQQIRIQASGGLSEA
                     EIEKMVKEAAEHAEEDKQRRAVIEARNQADSLIYSTEKSLKEYGDKLEAADRTAIEGA
                     ITELKGVLESEDPEAIKAKSDALAQVAMKLGEAMYKSQQSEGGDGDGGDAGGTSASGD
                     DVVDAEFEEVDDEKKKSA"
     gene            1358..2500
                     /gene="dnaJ"
                     /locus_tag="P5558_16420"
     CDS             1358..2500
                     /gene="dnaJ"
                     /locus_tag="P5558_16420"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRY25955.1"
                     /translation="MAKRDFYETLGVGKGADEADLKRAYRKLAMQYHPDRNPGDSAAE
                     ARFKEVNEAYEILKDPQKRAAYDQFGPQAFEQGMGGRGGAAGGFDFTSFADVFDDLFG
                     DFVGGGGGRRRGSSPRGNDLRYNLTISLEEAFSGKKAQVRVPTSVACDACDGSGSEGS
                     AEPAACPTCRGAGRVRAQQGFFTVERGCPTCQGSGRVVTNPCRTCSGSGVVHKEKTLA
                     INVPPGVEDGTRIRLSGEGEAGLRGGQPGDLYIFVTVAPHRLFRREGHNIFCRVPIPM
                     TTAALGGDVEVPTIEGKRVRLAIPGGTQTGKQIRLRSKGMTELHGQARGDMFVEIIVE
                     TPVNLTKRQQELLREFDGAGEKSNNHHPESDGFFAKVRELWDDLTD"
     gene            2563..3093
                     /locus_tag="P5558_16425"
     CDS             2563..3093
                     /locus_tag="P5558_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25956.1"
                     /translation="MPMASRETPVRESDRQDATDDVEALLRPVPAGRPRWAGREFLLG
                     ALSGVALPVLMLAVSLGMWLLRGDISPDGGLVVRPAAWLLAVAALIAGLILALAVASF
                     VKTRRQQRTNVALHEVVLTRLTRAEGSLAGARGLPLVHGTTAGHGALGNGRPGRHPPA
                     VGPDRALPRDGWWRDG"
     gene            3167..3649
                     /locus_tag="P5558_16430"
     CDS             3167..3649
                     /locus_tag="P5558_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25957.1"
                     /translation="MDPEHESDLGFLAEIAATPSDVLVRVYRPWQLAAAKATAAAVLA
                     RLRGQADILGRLPRRSRKDHFLEAGGWMFGLASCAFTVWSVAIDGPTYVAAASMFASS
                     GGFAVAILQRDNVREVEAARGEALQAVLHQVTLVELAIERLGSLGSAVVADASAPLRE
                     "
     gene            complement(3680..3922)
                     /locus_tag="P5558_16435"
     CDS             complement(3680..3922)
                     /locus_tag="P5558_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25958.1"
                     /translation="MNAVLSREPVKALCVEWNQTAAMAEARMRAGGCDMLQVVDGRRL
                     LGHITRADIDRCRRHGNWLDAVLVVDLVGEGARSGS"
     gene            complement(3919..4425)
                     /locus_tag="P5558_16440"
     CDS             complement(3919..4425)
                     /locus_tag="P5558_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25959.1"
                     /translation="MSVRAVNGRMSWGVATMGQATPCRVQWVTRRTDLSRLVDRMADA
                     GATLVSRSACEPTTGDLDELGDAETGFLSIAELGIDSDLLTSHLANTARGLSAGETMI
                     IDLTGSRLRVALLPVGGTHGVIIREPSGVRRLARPALGFGRNAARRLIDRFVQPSANQ
                     TTNRGAAA"
     gene            complement(4500..4934)
                     /locus_tag="P5558_16445"
     CDS             complement(4500..4934)
                     /locus_tag="P5558_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY25960.1"
                     /translation="MSKRPVREVIQRDTLVRADPTTTVKAAADEMARCRCGSILVMEG
                     EAVVGIFTERDLLMRVVHAGLDPAETKVGEVMTKAPDTIDADAPIGDAIRAMDQFSYR
                     YLPVIDSGRCIGVISTRHLPFGAVIGMAWEIDERHRLAERIW"
     gene            complement(5080..5511)
                     /locus_tag="P5558_16450"
     CDS             complement(5080..5511)
                     /locus_tag="P5558_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY25961.1"
                     /translation="MPRRPVRDFLARDTLLKAEPEETVRDAAIGMAAHHCGSVLIARG
                     DALVGIFTERDLLSRVVAAGRDLDTPLIEVMTANPDTIAADAPIKDAIRQMNECGYRH
                     LPVIENDRVLGILSIRDLPFAEIALMEKELDDRQSLAERIW"
     gene            complement(5604..6107)
                     /locus_tag="P5558_16455"
     CDS             complement(5604..6107)
                     /locus_tag="P5558_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY25962.1"
                     /translation="MFTKILAAVDGSGHAGKAVAIAGSLAGRYDADLVIAHVLTGQDV
                     PDELRRMAEVEHLVEPRPTEPARLGNLSVGSATKVDEGRLAAAVGARVLERAVAAAKR
                     EGARRVTPLQLSGDAADALLEAAKEQGADLVVVGSRGFGALGSLVMGSVSSKLAHHLE
                     VPCLTVK"
     gene            complement(6279..7124)
                     /locus_tag="P5558_16460"
     CDS             complement(6279..7124)
                     /locus_tag="P5558_16460"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HRY25963.1"
                     /translation="MKLLRYGPKGQEKPGLLDAAGTIRDLSGVIADIDGTTIGKDGLA
                     RLAALDPASLPAVGGSPRLGACVGKVGKFICIGLNYADHAAESGMAVPPEPVIFMKCT
                     SAICGPDDDVEIPRKSKKTDWEVELGVVIGKAAKYVEEKDALDHVAGYCVINDVSERE
                     FQAERAGQWTKGKSHDTFGPTGPWLVTKDEVPDPQDLKMWLEVDGKRYQDGSTRTMVY
                     KVPFLVHYLSQFMSLQPGDIISTGTPPGVGLGIKPNPVFLKAGQTMRLGIQGLGEQQQ
                     RTVQA"
     gene            complement(7157..8422)
                     /gene="murA"
                     /locus_tag="P5558_16465"
     CDS             complement(7157..8422)
                     /gene="murA"
                     /locus_tag="P5558_16465"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRY25964.1"
                     /translation="MDRILIKGGVPLQGEVAISGAKNAALPLMAACLLTDQPLELDNV
                     PDLADIGSMQRLLQVLGVEARLLPGAPGRMTLACENVLSTTAPYDLVRRMRASVLVLG
                     PLVAREGIARVSLPGGCAIGTRPIDLHLAGLEALGATIELADGYVTATAPGGLVGGVI
                     RFAQPSVGATENLMMAAALAKGTTEIHGAAREPEIVDLARLLEAMGAGIEGAGSSVLR
                     IEGRPTLGGARHHILPDRIEAGTYVMAAAITGGRVRLTRCRPDLLGAALEKLEEAGVS
                     IEPTEDGMVASRANGALSGVDVMTRPFPGFATDLQAQFMAMMCVAEGASMITETIFEN
                     RFMHAPELLRMGANINVHGGTALVRGVSRLNGAPVMATDLRASVSLVLAALAAEGETE
                     INRVYHLDRGYEQIERKLGNLGARVERVA"
     gene            complement(8528..9889)
                     /locus_tag="P5558_16470"
     CDS             complement(8528..9889)
                     /locus_tag="P5558_16470"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRY25965.1"
                     /translation="MIQPERIRDLNDAAERGDGSYVLYWMQQAQRVGTNHALGLAIEL
                     ANARGLPVVVAFGLMDDYPEANRRHYAFMLEGLAEVAAELRERSIRFVLRHGEPPRVA
                     LALAKEAAILVCDRGYLRHQVAWRRKVADEAGCRVVEVESDVVVPVETASDKAEYGAR
                     TIRPKIQRLRDDFLVGFTPEAVEHGSLDLDLESDFEALDPEAMLDRLKLDRSVDRVSR
                     FKGGAGPARERLAAFIEEKLDGYADGRNEPVARQCSELSPYLHFGQISPLDLALAAKA
                     AEGVDAEDRSGFLEELIVRRELSMNFVAFTQDYDSYAMLPGWARKTLGEHKRDKREHL
                     YDEAALAAGETHDPYWNAAMREMRVSGYMHNYMRMYWGKKIIEWSKTPEDAFATTLAL
                     NNRWFLDGRDPASFANVGWLFGLHDRAWTERPVFGKIRYMNDRGLKRKFDIDAYVKWT
                     ETL"
     gene            complement(9900..10304)
                     /locus_tag="P5558_16475"
     CDS             complement(9900..10304)
                     /locus_tag="P5558_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY25966.1"
                     /translation="MSDYAESPDPTGVDGVAASGQDPFAGLSSDLMESAGRATAFLKT
                     VANTNRLMILCCLVGTERSVSELETLLGMRQPALSQQLARLRQDDLVRTRRDGKMIFY
                     RLNGSEAVELIGLLHRLFCEPAGRSGHRGSRA"
     gene            complement(10383..>10720)
                     /locus_tag="P5558_16480"
     CDS             complement(10383..>10720)
                     /locus_tag="P5558_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25967.1"
                     /translation="PRAALTRLFQLELKSARNAGQPRGCLLAEARRVCHRLPAAVAAE
                     TRAAAGELEEALFAMFERARAAGRLAATADCRALARFYAAVARGVAGTDPEPLEDIAR
                     IGVALLEAA"
BASE COUNT         1686 a         3643 c         3726 g         1665 t
ORIGIN      
        1 tccggctgat cgactacatg gccgacgagt tcaagaagga gcagggcatc gacctgcgct
       61 ccgacaagct cgccttgcag cgcctcaagg aggcggccga gaaggccaag atcgagctct
      121 cctcgacgca gcagaccgag gtcaacctgc cgttcatcac cgccgaccag agcggcccca
      181 agcatctgac catgaagatc agccgggcca agctcgagat gctggtcgac gacctgatcc
      241 agcggaccat cgagccgtgc aagaaggccc tgaaggatgc cgggctcgcc gcctcggaca
      301 tccacgaggt gatcctcgtc ggcggcatga cccgcatgcc gaaggtcgtg gagacggtga
      361 agcagctctt cggcaaggag ccgcacaagg gcgtcaaccc cgacgaggtg gtggccatgg
      421 gtgccgccat ccaggccggc gtgctggccg gtgacgtcaa ggacgtgctg ctgctcgacg
      481 tgacgccgct gtcgctcggc atcgagaccc tgggtggcgt cttcacccgg atcatcgatc
      541 gcaacacgac gatcccgacc aagaagagcc aggtgttctc gaccgccgac gacaaccaga
      601 gcgcggtgac catccgggtc ttccagggcg agcgcgagct cgcggcccag aacaagctgc
      661 tcggccagtt cgatctcgtc ggcatcccgc cggcgccgcg cggcatgccg cagatcgagg
      721 tgaccttcga catcgacgcc aacggcatcg tcaatgtctc ggccaaggac aaggccaccg
      781 gcaaggagca gcagatccgc atccaggcct cgggcgggct cagcgaggcc gagatcgaga
      841 agatggtcaa ggaagccgcc gagcacgccg aggaggacaa gcagcgccgg gcggtgatcg
      901 aggcgcggaa ccaggccgac agcctgatct actcgaccga gaagagcctg aaggaatacg
      961 gcgacaagct cgaggccgcc gaccggacgg cgatcgaggg tgcgatcacc gagctcaagg
     1021 gcgtgctcga gagcgaggac cccgaggcga tcaaggcgaa gtcggacgcg ctggcgcagg
     1081 tcgccatgaa gctcggcgag gcgatgtaca agtcgcagca gagcgagggc ggcgacggcg
     1141 acggcggtga cgccggcggc acgtcggcca gcggcgacga cgtggttgat gccgagttcg
     1201 aagaggtcga cgacgagaag aagaagtccg cctgagccac gccgtggtgg acgccttcgc
     1261 ggcaacggga tgcggcaggg accgggtcgg cgacgacccg gtccctcgcc gttccgccgc
     1321 cgagattccc aacgatctac actaggaccg agcgttcatg gccaagcgcg acttctacga
     1381 gaccctcggc gtcggcaagg gcgccgacga ggcggacctc aagcgcgcct atcgcaagct
     1441 cgccatgcag tatcaccccg accgcaaccc gggcgacagc gcggccgagg cgcggttcaa
     1501 ggaggtcaac gaggcctacg agatcctgaa ggacccgcag aagcgggccg cctacgacca
     1561 gttcgggccg caggccttcg agcaggggat gggcggccgg ggcggggccg ccggcggctt
     1621 cgacttcacc agcttcgccg acgtcttcga cgacctgttc ggcgatttcg tcgggggcgg
     1681 cggcggccgg cgccgcggct cgagtccgcg cggcaatgat ctgcgctaca acctgacgat
     1741 cagcctcgag gaagccttct ccggcaagaa ggcgcaggtg cgcgtcccga cctcggtcgc
     1801 ctgcgacgcc tgcgatggca gcggcagcga gggcagcgcc gagcccgccg cctgccccac
     1861 ctgtcgcggg gccgggcgcg tgcgggccca gcagggcttc ttcaccgtcg agcgcggctg
     1921 cccgacctgc cagggcagcg gccgggtggt gaccaacccc tgccggacct gcagcggcag
     1981 cggcgtcgtc cacaaggaga agacgctcgc gatcaacgtc ccgccgggcg tcgaggacgg
     2041 cacgcgcatc cgcctctccg gcgagggcga ggcgggcctg aggggtggtc agccgggcga
     2101 tctctacatc ttcgtcaccg tcgcacccca tcgcctgttc cgccgcgagg gccacaacat
     2161 cttctgccgg gtgcccatcc cgatgaccac ggcagccctc ggcggcgatg tcgaggtgcc
     2221 gaccatcgag ggcaagcggg tgcgcctcgc cattcccggc ggcacccaga ccggcaagca
     2281 gattcgcctg cgcagcaagg gcatgaccga gctgcacggc caggcgcggg gcgacatgtt
     2341 cgtcgagatc atcgtcgaga cgcccgtcaa cctgaccaag cgccagcagg agctcctgcg
     2401 cgagttcgac ggcgccggcg agaagtcgaa caaccaccat cccgaatcgg acggcttctt
     2461 cgccaaggtc cgcgagctct gggacgacct gacggactag ccggctttgc gtcgagcact
     2521 gacccggcgt aaattgtcgc gtggcgagac ttcgaacggg gcatgccgat ggccagccgg
     2581 gaaaccccgg tgcgggagag tgaccggcag gatgcgacgg atgatgtcga ggccctgctg
     2641 cggcccgttc ctgccgggcg accgcgctgg gccggtcgcg agttcctgct cggcgccctg
     2701 agcggcgtgg cgttgccggt gctgatgctg gcagtgtctc tcggcatgtg gctgttgcgc
     2761 ggcgatatct caccggatgg cggcctcgtc gtgcgccccg ccgcgtggct gctcgctgtc
     2821 gcggccctga ttgccggtct gatcctggcc ctggccgtgg cgtcgttcgt caagacgaga
     2881 cgtcagcagc gcaccaacgt ggccctgcat gaggtcgtcc tcacgcgact gaccagggcg
     2941 gaaggatcgc tggccggggc tcgaggcctc ccgctcgtcc atggcaccac ggccggacat
     3001 ggtgcgctcg gcaatggtcg accgggccgt catccgcctg cagttggacc agatcgagcg
     3061 ctacctcggg atggctggtg gcgggacggg tgaaggcaat gacccggccg ccgtgcccgg
     3121 ggtcgcacag ggcaaggtct gaagcacccc gcccggaccc tcgccagtgg atccggagca
     3181 cgaatccgac ctcggttttc tcgccgagat cgccgcgaca ccctccgatg ttctcgtccg
     3241 ggtctaccga ccctggcagc tcgctgccgc caaggctact gccgctgctg tcctggcgcg
     3301 tttgcgcggc caagccgata ttctgggacg actgccgcgt cggtcgcgca aggaccattt
     3361 tctggaggct ggcgggtgga tgttcggtct ggccagttgc gccttcacgg tgtggagcgt
     3421 cgccatcgac ggccccacct atgttgctgc cgccagcatg ttcgcctcca gcggtggctt
     3481 tgcggtcgcc atattgcagc gtgacaacgt ccgcgaggtc gaggcggcgc gcggggaagc
     3541 actgcaggcg gtgctccacc aagtcacgct ggttgaactg gccatcgagc gtcttggctc
     3601 gctcgggtca gcggttgtcg ccgacgcttc cgcccctctg cgcgaataac aggctgatgc
     3661 aggcggcttg cagtgccgct cagctacccg agcgggctcc ctcgccgacc agatcgacga
     3721 ccaggacggc gtcgagccag ttgccgtgcc ggcggcagcg atcgatgtcg gcgcgggtga
     3781 tgtggccgag gagccgccgg ccatcgacga cctgcagcat gtcgcaaccg ccggcccgca
     3841 tcctggcctc ggccatggcg gccgtctggt tccattccac gcaaagggcc ttgacgggct
     3901 ctctcgacag gacggcgttc atgctgcggc tccccggttg gtggtctgat tggcagaggg
     3961 ctggacgaat cgatcgatca ggcggcgggc ggcattgcgg ccgaagccca gggccggccg
     4021 ggcgaggcgt cgcacgccgg acggttcccg gatgatgacg ccgtgcgtcc cgccgaccgg
     4081 cagcagcgcg acgcgcagcc gcgagccggt gaggtcgatg atcatggttt cgcccgccga
     4141 gagcccgcgg gccgtgttgg ctaggtggga tgtgagcaga tcggagtcga tcccgagctc
     4201 ggcaatgctg aggaagccgg tctcggcatc gcccaattcg tcgagatcgc cggtcgtggg
     4261 ctcgcaggcg ctgcgcgaga cgagcgtggc accagcatcc gccatgcggt cgacgagccg
     4321 cgagagatca gttcgccgcg tgacccactg cacacgacat ggcgttgcct gtcccatcgt
     4381 tgctaccccc cacgacatcc ggccgttgac ggcacggaca ctcaacttgc gaggctacga
     4441 taggttcctc ttcccggcaa tgacctagat caaacccgcg accattggca ccggtgctct
     4501 caccagatcc gctcggccag ccggtggcgc tcgtcgatct cccaggccat gccgatgacc
     4561 gcgccgaacg gcaagtggcg ggtcgagatg acgccgatgc agcgcccgct gtcgatgacc
     4621 ggcagatagc ggtagctgaa ctggtccatg gcacggatgg cgtcgccgat cggcgcatcg
     4681 gcgtcgatcg tgtccggtgc cttggtcatc acctcgccca ccttcgtctc ggccggatcg
     4741 aggccggcat gaacgacgcg catcaagaga tcgcgctcgg tgaagatgcc gaccaccgcc
     4801 tcgccctcca tgacgaggat gctgccgcaa cggcatctcg ccatctcgtc ggcggcggcc
     4861 ttgacggtgg ttgtggggtc ggcgcgcacg agggtatccc gctggatgac ctcgcgaacc
     4921 ggtctcttgg acatggtttc actcccgatg ctcgcccggt gcccggacga ttgctgctcc
     4981 tcgcaatcct tgcctagcag ggcgatggca cggggcgcca tgatcaggat caatcgcggc
     5041 gcgggcgatt gcccggatcc tgcgtgccgc ccaattcgtc taccagatac gttcggcgag
     5101 cgactggcgg tcgtccagct ccttctccat gagcgcgatc tcggcgaagg gcaggtcgcg
     5161 gatggagaga atgccgagga cccgatcgtt ctcgatgacc ggcaaatgcc ggtagccgca
     5221 ctcgttcatc tggcggatgg cgtccttgat tggggcatcg gcggcgatgg tgtcggggtt
     5281 ggcggtcatc acctcgatga gtggcgtgtc gaggtcgcgg ccggcagcca ccacccggga
     5341 gagcaggtcg cgctcggtga agatgccgac cagcgcatcg ccgcgggcga tcagcacgct
     5401 gccgcagtgg tgcgccgcca tgccgatcgc ggcatcgcgc acggtctcct ccggctcggc
     5461 cttgagcagc gtgtcgcggg cgaggaagtc acggacgggg cgacgcggca tggttcctgt
     5521 cctccaactg atttcgcctg atagttgatc aagaagtcaa cagcatccct gaatttttta
     5581 caaggccatt ttttctgtcc cgatcatttc acggtcaggc aaggcacctc caggtggtgg
     5641 gcgagcttgc tcgagacgct gcccatgacg aggctgccga gcgcgccgaa gccgcggctg
     5701 ccgacgacga cgagatcggc accctgctcc ttggccgcct cgagcagcgc atcggcggcg
     5761 tcgccggaga gctgcagcgg cgtcacccgc ctcgcaccct cgcgcttcgc ggcggcgacc
     5821 gcccgctcca gcaccctcgc accgaccgcg gcggcgaggc gcccttcgtc gaccttcgtg
     5881 gccgagccca cggacagatt gccgaggcgt gccggctcgg tcggccgcgg ctcgaccaga
     5941 tgctcgacct cggccatgcg cctgagctcg tccggcacgt cctggcccgt caggacatgg
     6001 gcgatgacga gatcggcatc gtagcggccg gccaggctgc cggcgatcgc cacggccttc
     6061 ccggcgtgcc cggagccgtc gacggcggcg agaatcttcg tgaacatcgt cgctcccccc
     6121 tcgttcgcgc ggaatccggg aaaggataga cgatcgggcg cagcgggttc ttgcgctgga
     6181 tcaatctggc ggcggcaggc aggccgcggc accccgcgcg gccggacgag atcgaagtga
     6241 tcgacctcgc cggcggcgcg gggccgggct ggtgtcgatc aggcctggac ggtgcgctgc
     6301 tgctgctcgc cgaggccctg gatgccgagc cgcatggtct ggccggcctt gaggaagacg
     6361 gggttcggct tgatgccgag gccgacgccg ggcggcgtgc cggtcgagat gatgtcgccc
     6421 ggctgcaggc tcatgaactg cgagaggtag tggaccagga acggcacctt gtagaccatg
     6481 gtccgggtcg agccgtcctg gtagcgcttg ccgtccacct cgagccacat cttgaggtcc
     6541 tggggatcgg gcacctcgtc cttcgtcacg agccatgggc cggtcgggcc gaacgtgtcg
     6601 tggctcttgc ccttggtcca ctggccggcg cgctcggcct ggaactcgcg ctccgagacg
     6661 tcgttgatca cgcagtagcc ggcgacgtgg tcgagggcgt ctttctcctc gacatacttg
     6721 gccgccttgc cgatcaccac gccgagctcg acctcccagt cggtcttctt cgacttgcgc
     6781 gggatctcga catcgtcatc ggggccgcaa atggcgctcg tgcacttcat gaagatgacc
     6841 ggctccggcg gcacggccat gccgctctcg gcagcatggt cggcgtagtt gaggccgatg
     6901 cagatgaact tgccgacctt gccgacgcag gcgccgaggc gcgggctgcc gccgaccgcg
     6961 ggcaggctgg ccggatcgag ggcggcgagc ctggccaggc catccttgcc gatggtcgtg
     7021 ccgtcgatgt cggcgatcac gcccgaaagg tcgcggatcg tgccggcggc atcgagcagg
     7081 ccgggctttt cctggccctt ggggccgtag cgcaagagct tcatcgcgtt tctctcccgt
     7141 ttgaaaaaaa ggtcgttcag gcgacccgct cgacgcgggc gcccagattg ccgagcttgc
     7201 gctcgatctg ctcgtagccg cgatcgagat ggtagacccg gttgatctcg gtctcgccct
     7261 cggcggcgag ggccgccagc accagcgaca ccgaggcgcg caggtccgtc gccatgaccg
     7321 gcgccccatt gagacggctg acgccgcgga ccagcgccgt gccgccgtgg acgttgatgt
     7381 tggcccccat gcgcaggagc tcgggcgcat gcatgaagcg gttctcgaag atcgtctcgg
     7441 tgatcatcga ggccccctcg gcgacgcaca tcatcgccat gaactgggcc tgcaggtccg
     7501 tggcgaagcc cgggaagggc cgggtcatga cgtcgacgcc gctgagcgcg ccattggcgc
     7561 ggctggccac catcccgtcc tcggtgggct cgatggaaac accggcctcc tccagcttct
     7621 ccagcgcggc ccccagcagg tcggggcggc aacgggtgag ccgcacccgg ccgccggtga
     7681 tcgccgccgc catgacatag gtgccggcct cgatccggtc cggcaggatg tggtggcggg
     7741 caccgccgag cgtcggccgg ccctcgatcc tgaggacgga gctgccggcg ccctcgatcc
     7801 cggcacccat ggcctcgagc agcctggcga gatcgacgat ctcgggctcg cgcgcggcgc
     7861 cgtggatctc cgtcgtgcct ttggcgagcg ccgccgccat catcaggttc tcggtggcgc
     7921 cgacggaggg ctgggcgaag cggatcacgc cgcccaccag gccgcctgga gccgtcgccg
     7981 tgacgtagcc gtcggccagc tcgatcgtcg cacccagggc ctcgagcccg gcgagatgca
     8041 ggtcgatcgg ccgcgtgccg atggcgcagc cgccgggcag cgacaccctg gcgatgccct
     8101 cgcgcgcgac cagcgggccg agcaccagga ccgaggcccg catccggcgg acgaggtcgt
     8161 agggggcggt ggtcgagagc acgttctcgc aggcgagcgt catccgcccg ggtgcgccgg
     8221 gcagaagccg cgcctcgacc ccgagcacct gcaagaggcg ctgcatcgag ccgatgtcgg
     8281 cgaggtcggg cacgttgtcc agctcgagcg gctggtcggt caggaggcag gcggccatga
     8341 gcggcagggc ggcgttcttc gccccgctga tcgccacctc gccctggagc ggcacgccgc
     8401 ccttgatgag gatacggtcc atggatcgtc ccgagaggcg gggcccatgc cccgcgagcg
     8461 gcccctaagt gccaagcggc gcggctgagg gcaagcgtct tctctcaact ttggagcgat
     8521 cggccgctca gagggtctcc gtccacttca cataggcgtc gatgtcgaac ttccgcttca
     8581 gcccacggtc gttcatgtag cggatcttgc cgaagaccgg ccgctcggtc caggcgcggt
     8641 cgtgcagccc gaagagccag ccgacattgg cgaaggaggc ggggtcgcgc ccgtcgagga
     8701 accagcggtt gttcagcgcc agcgtggtgg cgaacgcatc ctcgggtgtt ttcgaccatt
     8761 cgatgatctt cttgccccag tacatccgca tgtagttgtg catgtagccc gagacgcgca
     8821 tctcgcgcat cgctgcgttc cagtaggggt cgtgggtctc gcccgctgcg agcgccgcct
     8881 cgtcgtagag atgctcgcgc ttgtcgcgct tgtgctcgcc cagcgtcttc ctcgcccagc
     8941 ccggcagcat ggcgtagctg tcgtagtcct gcgtgaaagc gacgaagttc atcgaaagct
     9001 cgcggcggac gatcagctcc tcgaggaagc ccgaccggtc ctcggcgtcg acgccctcgg
     9061 ccgccttcgc ggcgagcgcc aggtcgagcg gcgagatctg gccgaaatgc agataggggc
     9121 tgagctcgga gcattgccgg gcgacgggct cgttgcggcc atccgcatag ccgtccagct
     9181 tctcctcgat gaacgcggcg aggcgctcac gggccgggcc cgcgccgcct ttgaaccggc
     9241 tcacccggtc gacgctgcgg tccagcttca gccggtcgag catggcctct ggatcgagcg
     9301 cctcgaaatc gctctcgaga tcgaggtcga gcgagccgtg ctccaccgcc tcgggcgtga
     9361 agcccacgag aaaatcgtcc ctgagccgtt ggatcttcgg ccggatggtg cgcgccccgt
     9421 attcggcctt gtcgctggcg gtctcgaccg gcacgaccac gtcactttcc acctcgacca
     9481 cccgacagcc ggcctcgtcc gcgaccttcc ggcgccaggc cacttggtga cggagatagc
     9541 cgcggtcgca gacgagaatg gccgcctcct tcgccagcgc cagggccacg cggggcggct
     9601 cgccgtggcg gagcacgaag cgtatactgc gctctcgaag ctcggccgcg acctcggcca
     9661 ggccctcgag catgaaggcg tagtggcggc gattggcttc gggatagtcg tccatgaggc
     9721 cgaaggccac gaccaccggc aggccgcgcg cgttggccag ctcgatggcg aggccgagcg
     9781 cgtggttcgt gcccacgcgc tgcgcctgct gcatccagta gagcacgtag cttccgtcac
     9841 cacgctcagc ggcgtcgttc agatctcgaa tgcgctcggg ctggatcatc cgggtcgagc
     9901 taggccctcg agcctcgatg tccagagcgg ccggccggct cgcagaagag gcgatgcagg
     9961 aggccgatca attccaccgc ctcgctgccg ttgaggcggt agaagatcat cttgccgtcc
    10021 cgccgggtcc tgaccaggtc gtcctggcgc agccgggcga gctgttgcga aagggccggc
    10081 tggcgcatgc cgagcagtgt ctcgagctcg ctcaccgacc gctcggtgcc gacgaggcag
    10141 cagaggatca tcaggcggtt ggtgttcgcc acggtcttga gaaaggcggt ggcccgcccg
    10201 gcgctctcca tgagatcgct cgacagaccg gcgaacgggt cctggccaga ggcggcaacg
    10261 ccgtcgactc cggtcgggtc aggcgattca gcatagtccg acacggtggt gccttcgact
    10321 ttctggtgca caccggaaca tatatagcgt ccagcacgtc gctgcgcgag cctcggccat
    10381 gctcaggccg cctcgagcag cgccacgccg atccgcgcga tgtcctcgag cggctcgggg
    10441 tcggtgccag cgacaccccg cgccacggcg gcatagaacc gggccagcgc ccggcaatcg
    10501 gcggtggctg ccagccggcc ggcggcgcgg gcacgctcga acatcgcgaa aagggcctcc
    10561 tccagctcgc cggccgccgc ccgggtctcg gccgcgaccg ccgccggcag gcggtggcag
    10621 acccgtctgg cctcggccag caggcagccc ctgggctggc cggcattgcg cgccgatttc
    10681 agctcgagct ggaacaggcg ggtgagcgcc gcccgcgggt
//
LOCUS       DAOTFI010000173        10680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7351_length_10680_cov_6.278682, whole genome shotgun sequence.
ACCESSION   DAOTFI010000173 DAOTFI010000000
VERSION     DAOTFI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7351_length_10680_cov_6.278682"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="P5558_16485"
     CDS             complement(<1..434)
                     /locus_tag="P5558_16485"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HRY25968.1"
                     /translation="MDEGFWHRRWQKNEIGFHEGAANALLARHFGEVFGRGGRVFLPL
                     CGKTRDIGWLLAQGWRVAGAELSRLAVDQLFGDLGVEPAIAEFAGVTRCSADGIDIFV
                     GNILDLTAGLLGPVDVVYDRAALVALPAAMRERYATHLRQLT"
     gene            complement(485..1681)
                     /locus_tag="P5558_16490"
     CDS             complement(485..1681)
                     /locus_tag="P5558_16490"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRY25969.1"
                     /translation="MPRRTRSPDRLAISPARDLLPDEIGERMDGVLRPHAAEGDRIEA
                     FLPSPCVQNHAANLALLPDGSLACVWFGGTMEGMGDISIWLSTLLPGAERWSPARRLT
                     DDPAHSEQNPILFVAPDGMLWLFHTAQPGGRQDLCEIRCRRSGDGGASFGPAQRLGEF
                     RGVFVRQPLCIGPAGEWLLPAFRCVAPAGRPWTGAEDTALALVSGDGGEHWRALEVPE
                     SLGAVHMNMVEARSGPMRAFFRNRFATRVLQSVSADGGLAWSKPQPTALPNNNASIQA
                     IRRRDGRIAMVLNPVNALMSDQRRASLYDEIEGDDAAAGTGGAVWGVPRAPLSLVHSA
                     DDGASFAERRDLESGSGYCLTNNSKDGLNRECSYPSIVEDGEGALHIAFTYHRRASKH
                     VRLAPG"
     gene            complement(1721..3349)
                     /locus_tag="P5558_16495"
     CDS             complement(1721..3349)
                     /locus_tag="P5558_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY25970.1"
                     /translation="MSSNEDGAAFDYIVVGGGSAGCVVTNRLVDRQGARVLLLEAGWP
                     DDNPMIRMPAGFIKFLNGSKFLTEHLAVPQEQLGGRRPAIWQGNVLGGGSSVNAMVYM
                     RGRPADYDHWDEVTGGAGWSWADMLPYFLRQEANQRFNNPMHGIDGPLRVSDPVHVAE
                     MSYIYLRTMQAQGVKLVDDFNAGDQRGVGFMQTTTYPRVRCSAVRAFLDPVRGNRNLE
                     VKTRAKVTRVLFEGRRASGVEHRIDGATHTARATRAVILTAGAFVTPQLLMLSGIGPA
                     AHLREHGLEVLVDAPGVGANLHDHHEVPLIASTDRNYGYFKEDRGWRMLANGLNYLLF
                     KGGPVASGGVDACAFVNPQEPAGEPTIKLYCVPLVYGDKEVLNREPMPGVTFTSCVLQ
                     PKARGSVRLRSADPLDLPLVDPNYLGHPDDMRLQVDALLHARTVMRTRPMADTIVEEL
                     LPGAAVVDREGLEEHCRRTVKTNWHPCGTARMGRAGDPMAVLTPDLAVKGVEALHVLD
                     ASLMPTVPHGNTNAPTMAAADRAVDKLLGRSQPS"
     gene            3536..3730
                     /locus_tag="P5558_16500"
     CDS             3536..3730
                     /locus_tag="P5558_16500"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY25971.1"
                     /translation="MGHGHAERTPFGRWGRPEEVARVIAFLAHPDSGFVTGVVWPVDG
                     GFSMRGDPGEDSGPRSVQMP"
     gene            3873..4748
                     /locus_tag="P5558_16505"
     CDS             3873..4748
                     /locus_tag="P5558_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRY25972.1"
                     /translation="MIPGNFAYHRPADVAAAARLLQEHGDEAQLLAGGHSLIPMMKLR
                     MAQPSHLIDLQDIAGLKGISRDGARLVIGAMTTQHELIGDALVAAAVPLLREAALQIA
                     DPQVRYVGTIGGNVANGDPGNDMPGVMQCLDASFELTGPGGTREVAARAFYEAAFTTA
                     RAEDEILTRIIVPIPKPGSGYAYLKQKRKIGDYATAAAAVLLAVESGRCTSAAIAMTN
                     LAATPVFTEAAGAALVGTTLDEAAVGAAVAAMLAAIDPATDNRGPVEFKKHVAGLMLR
                     RAIAEAAGRAGRGTA"
     gene            4748..5242
                     /locus_tag="P5558_16510"
     CDS             4748..5242
                     /locus_tag="P5558_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY25973.1"
                     /translation="MDKLHVKLQVNGKPVEALVEPRLLLIHFLREELGLTGPHIGCET
                     SHCGACTVDIGGRSVKSCTVFTAQANGAEITTIEGIEGADGGLHALQEMFREHHGLQC
                     GFCTPGMITRAWRLLEENPDPSEAEIRQGMAGNLCRCTGYQNIVKAIRAAADARQAQQ
                     EAAE"
     gene            5239..7659
                     /locus_tag="P5558_16515"
     CDS             5239..7659
                     /locus_tag="P5558_16515"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HRY25974.1"
                     /translation="MTEQSPVDLDRAEKLQGLGCRRKRVEDARFTQGKGNYVDDMKLK
                     GMLFGDFVRSPYAHARVKSIDAEAAKALPGVVAVLTADDLRPLNLHWMPTLAGDKQMV
                     LADGKVLYQGQEVAFVIAENRYIAADAVDLVEVEYEALPVVTDPFKALADDAPVLRED
                     IADQKEGAHGPRRHPNHIFTWEQGDKAATEAVLAGAEVVAEEMIYYHRTHPCPLETCG
                     CVASMDKVNGKLTLWGTFQAPHAVRTVASLISGIAEHNIRVVSPDIGGGFGNKVGVYP
                     GYICAVVASIVTGVPVKWVEDRMDNLMTTAFARDYWMQGKIAATKDGRITGLWCHVTA
                     DHGGFDACADPTKFPAGFFNICTGSYDIPTAWLAVDGVYTNKAPGGVAYRCSFRVTEA
                     SYFIERMVDILAIKLGMDPVELRLKNFIRKDQFPYQSALGWEYDSGDYHTAMEKALAA
                     VDYAGLRREQAERLDAFKRGETRKLIGIGVSFFTEIVGAGPVKNCDILGLGMFDSCEI
                     RIHPTGSAIARLGTISQGQGHATTFAQILASEIGIPADAITVEEGDTDTAPYGLGTYG
                     SRSTPVAGAATAMAGRKIRAKAQMIAAYLLEVHDDDIEWDIDRFVVKGAPERFKTMTE
                     IAFAAYNQAIPGLEPGLEAVSYYDPPNMTYPFGAYVCVMEIDVDTGETEIRRFYALDD
                     CGTRINPMIIEGQVHGGLTEALAIAMGQEIAYDENGNVMTATLMDFFMPTAVETPTWE
                     TDHTVTPSPHHPIGAKGVGESPNVGGVSAFSNAVNDAFRPFGLTHAQMPHDHWRIWRT
                     AQQLGLHG"
     gene            7685..8560
                     /locus_tag="P5558_16520"
     CDS             7685..8560
                     /locus_tag="P5558_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY25975.1"
                     /translation="MDHLDLQRRLAETGYVAGEPLAMALHLSLVLGRPLLLEGEAGVG
                     KTEIAKALATIQKTRLIRLQCYEGLDAAAAIYEWNYQRQLLAIRAAAETARVEEASEE
                     RLFSERYLLRRPLLEAILQPVPPVLLIDEIDRADEAFEAFLLEILADFQITVPELGTI
                     EAVSRPHVVLTSNATRELSDALRRRCLYAFVDYPDPAMELAILAARAPELEPALAAQI
                     VGFVQGLRREELEKTPGIAETLDWVAALSGLGINDLAASLATVQASLVCLLKTSADQA
                     AVTPEVIARLVDRAA"
     gene            8571..9761
                     /locus_tag="P5558_16525"
     CDS             8571..9761
                     /locus_tag="P5558_16525"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY25976.1"
                     /translation="MQPTRFAARARGPAERLAGFMAHLRLNGIAVGPAETTRALAGLA
                     AIDATDLGRARLALKTLVTTTPEDWNRFDELFDAYWLNTGRVRTRVTGSPPAGQKPPA
                     LWSRHLPQAEGAAPVTGAPTDAATGEDDDPADGRGEGRLVATGQPATARRDLRSLMAE
                     ADLAEAETIAERLARAIRERHARRRRQARLGPEIDLRRTLRRSLARGGEPLDLARRRR
                     REPPLRIVALLDVSGSMTVHARVFLAFLKGLVGADSRADAYLFHTSLVRVTEALTARD
                     PLTAATRLSLLTRGMGGGTRIGQSLATFNATYAKRAVNGRTAVLILSDGYDTDPPELL
                     AEALARLRRRARRVVWLNPLKGWRGYAPVARGMAAAMPHLDAFLEASTLASLAALEAQ
                     FREL"
     gene            9766..10620
                     /locus_tag="P5558_16530"
     CDS             9766..10620
                     /locus_tag="P5558_16530"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HRY25977.1"
                     /translation="MAATDVFDVIERLRATGRPFCVATVVRTADVTSAKAGAKAVVTE
                     TGELIGHLGGACVAGAVARAARSALEEGAVRLIRVKPAATVAGAVDPDGAELFKSGCP
                     SGGTVDLLIEPYALPPLIAILGRSPVAKALATQARLLGYRVAAEVSGDSASDRFIENF
                     DLTPLALRPTDFVVVAAQGRGDLQALRAALGAPPVYVGMVASRKKAAVLLDRLRSEGM
                     DEARLARLDSPAGLDLGGVDPEEIALAIMAAIVRIRNRGRRATGPSGPETAGGGPDAA
                     GKEVDPTP"
BASE COUNT         1659 a         3810 c         3657 g         1554 t
ORIGIN      
        1 tcggtgagct gccgcagatg ggtcgcgtag cgttcgcgca tcgccgcggg cagggccacc
       61 agggcggcgc ggtcgtagac gacatcgacc ggcccgagca ggccggcggt gagatcgagg
      121 atgttgccga cgaagatgtc gatgccgtcg gcgctgcagc gcgtgacccc ggcgaattcg
      181 gcgatcgccg gctccacacc gagatccccg aagagctggt ccacggccag ccggctgagc
      241 tccgccccgg cgacccgcca gccctgcgcc aggagccagc cgatgtcgcg cgtcttgccg
      301 cagagtggca ggaacacccg cccgccccga ccgaacacct cgccgaaatg ccgggccaag
      361 agggcgttgg cggcaccttc gtgaaagccg atctcgttct tctgccagcg gcggtgccag
      421 aatccctcgt ccatgctcgc tcccgttgcg tccggtgccg ggcccaaggt gtagtcccgc
      481 ccgctcagcc gggcgcgagg cggacatgct tgctggcccg gcgatgatag gtgaaggcga
      541 tgtgcagggc accctcgccg tcctcgacga tggacggata ggagcattcc cggttgaggc
      601 cgtccttgga gttgttggtc aggcagtagc cgctgccgga ttcgagatcg cgccgttccg
      661 cgaagctggc accgtcatcc gccgaatgga cgaggctgag cggtgcgcgc ggcacgcccc
      721 agacggcgcc gccggtgccc gctgctgcgt cgtcgccctc gatctcgtca tagagcgagg
      781 cacgccgctg gtccgacatc agggcattga cggggttcag caccatggcg atccgcccgt
      841 cccgccgccg gatcgcctgg atcgacgcgt tgttgttggg aagtgccgtg ggctgcggct
      901 tcgaccaggc aaggccgcca tcggccgata cgctctggag cacccgggtc gcgaaccggt
      961 tccggaagaa ggcgcgcatc ggccccgagc gggcctcgac catgttcatg tgcacggccc
     1021 ccaggctctc cggcacctcg agggcgcgcc aatgctcccc gccatcgccc gagaccagcg
     1081 cgagtgcggt atcttccgcc ccggtccagg gccgcccggc cggcgccacg cagcggaatg
     1141 ccggcagcag ccattcgccg gccggaccga tgcacaaagg ctggcggacg aagacgcccc
     1201 ggaattcgcc gagccgttgc gcggggccga agctcgcccc gccatcgccc gagcggcggc
     1261 agcggatctc gcaaagatcc tgccggccgc cgggctgggc ggtgtggaac agccagagca
     1321 tcccgtccgg ggcgacaaag aggatcgggt tctgctcgga atgcgccgga tcgtcggtga
     1381 ggcgccgggc cgggctccag cgctcggcac ccggcagcaa ggtcgacagc cagatggaga
     1441 tgtcgcccat gccctccatc gtgccgccga accagacgca cgccagcgag ccgtccggca
     1501 gcaaagcgag attggccgcg tggttctgca cgcagggcga cggcaggaac gcctcgatcc
     1561 gatcgccttc cgccgcatgc ggccgcagca cgccatccat ccgctcgccg atctcgtcgg
     1621 gcagcaagtc gcgggcaggc gaaatcgcca gccggtccgg gcttcgcgtg cgcctgggca
     1681 tgtcggtccc tttccactgg gcccgcaggc gagcccgctc ctatgacggc tggctccggc
     1741 cgagcagctt gtcgaccgcc cggtcggcgg cggccatggt gggcgcgttg gtgttgccgt
     1801 gcggcacggt cggcatgagc gacgcatcga gcacgtggag agcctcgacg cccttgacgg
     1861 cgaggtcggg ggtgagcacc gccatgggat cgccggcgcg gcccatccgg gccgtgccgc
     1921 aggggtgcca gttggtcttg accgtgcgcc ggcaatgctc ctcgagcccc tcccggtcga
     1981 caaccgccgc acccggcagc aactcctcga caatggtgtc cgccatgggc cgggtgcgca
     2041 tcaccgtccg ggcatggagc agcgcgtcta cctgcaggcg catgtcgtcc gggtggccga
     2101 gatagttggg atcgaccagc ggcaggtcga gaggatcggc cgagcgcagc cgcacggagc
     2161 cccgtgcctt cggctgcagg acgcagctcg tgaaggtgac cccgggcatc ggctcccggt
     2221 tcagcacctc cttgtcgcca tagaccagcg ggacgcagta gagcttgatg gtgggctcgc
     2281 ccgccggctc ctgcgggttg acgaaggcgc aggcgtcgac cccgccgctg gccacgggcc
     2341 cgcccttgaa gagcaggtag ttcaggccgt tggcgagcat ccgccagccg cgatcctcct
     2401 tgaaatagcc gtaattgcgg tcggtggagg cgatcagcgg cacttcgtgg tggtcgtgca
     2461 gattggcgcc gacgcccggc gcatcgacca gcacctcgag cccgtgctcc cgcaggtgcg
     2521 ccgccgggcc gatgccggag agcatcagga gctgcggtgt cacgaaggcg ccggcagtca
     2581 ggatgaccgc gcgcgtcgcc cgtgccgtgt gggtcgcccc gtcgatccgg tgctccaccc
     2641 cgctcgcccg ccggccctcg aagaggacgc gggtcacctt cgccctcgtc ttcacctcga
     2701 ggttccggtt gccgcgcacc ggatcgagga acgcccggac ggcactgcag cgcaccctgg
     2761 ggtaggtcgt ggtctgcatg aagccgacgc cgcgctggtc gccggcgttg aagtcgtcga
     2821 ccagcttcac gccctgcgcc tgcatggtcc tgaggtagat gtagctcatc tcggcgacgt
     2881 gcaccgggtc ggagaccctg agcgggccgt cgatgccgtg catcgggttg ttgaaccgct
     2941 ggttggcctc ctggcggagg aaatagggca gcatgtcggc ccaagaccag ccggcgccgc
     3001 cggtcacctc atcccagtgg tcgtagtcgg caggccggcc gcgcatgtag accatggcat
     3061 tgaccgagct gccgccgccc agcacgttgc cctgccagat cgccggccgg cggccgccga
     3121 gctgttcctg cggcaccgcc aggtgctcgg tcaggaactt gctgccgttc aggaacttga
     3181 tgaagccggc gggcatgcgg atcatcgggt tgtcatcggg ccagcccgcc tcaagcagca
     3241 gcaccctggc accctgccgg tcgacgaggc ggttggtcac gacacagccg gccgacccgc
     3301 cgccgacgac gatgtagtcg aaggcagcgc cgtcctcgtt cgagctcatg cgtcgtccct
     3361 cccctcgctc ggctttgcaa ccggcctcgg ccacgccgga cggcttgtca atcattgccc
     3421 gcagcacgga ttgccctcag gaagccatta tccagcaagg gggaaggaat cggataagag
     3481 agcaagaact ggcgggtcgg gtcgctctgg tgacgggggc ggcgcgcggc atcgggtggg
     3541 ccacgggcat gccgagcgta cgcccttcgg ccgctggggc cggccggagg aggtcgcccg
     3601 ggtgatcgcc tttttggcgc atcccgattc cggcttcgtc accggcgtcg tctggccggt
     3661 ggatggcggc ttcagcatgc gcggcgaccc cggcgaggat agcggcccgc gttcggtgca
     3721 aatgccctga atcgagacgc cggacggcac ggcgccagcg ggacggaaga acttatgcat
     3781 tgaatccgat ggatcgtgct ctatcctgcc agcgccgaac ggcgccccca ttgccgacgg
     3841 caatgccgca tcgggcggga ggccaagagg aaatgatacc gggcaacttc gcgtaccacc
     3901 ggccggccga cgtcgccgcc gcggcgcggc tcctgcagga gcatggcgac gaggcccagc
     3961 tgctcgcggg cgggcacagc ctgatcccga tgatgaagct gcgcatggcg cagccctcgc
     4021 atctgatcga cctgcaggac atcgccgggc tcaagggcat cagccgcgac ggcgcaaggc
     4081 tggtcatcgg cgccatgacc acccagcacg agctgatcgg cgatgcgctg gtggccgcgg
     4141 ccgtgccttt gctgcgcgag gcggccctgc agatcgcgga cccgcaggtc cgctatgtcg
     4201 gcaccatcgg cggcaacgtc gcgaatggcg atccgggcaa cgacatgccg ggcgtgatgc
     4261 aatgcctcga tgcatccttc gagctcacgg gtccgggcgg cacacgcgag gtcgcggcgc
     4321 gcgccttcta cgaggccgcc ttcacgacgg cacgcgccga ggacgagatc ctcacccgga
     4381 tcatcgtgcc gataccgaag cccggcagcg gctacgccta tctgaagcag aagcgcaaga
     4441 tcggcgacta cgccacggcg gccgccgcgg tgctcctcgc ggtcgagagc ggccgctgca
     4501 ccagcgccgc gatcgccatg accaacctcg ccgccacgcc ggtcttcacc gaggcggcgg
     4561 gtgcggccct cgtcggcacc acgctcgacg aggcggcggt cggtgccgcc gtcgccgcca
     4621 tgctggcggc gatcgacccg gccaccgaca accgcgggcc ggtcgaattc aagaagcatg
     4681 tcgccgggct gatgctgcgc cgggccatcg ccgaggccgc cggccgcgcc ggcaggggga
     4741 ccgcctgatg gacaagctgc acgtcaagct ccaggtcaac ggcaagccgg tcgaggccct
     4801 ggtcgagccg cgtttgctgc tgatccactt cctgcgcgag gagctcggcc ttaccggccc
     4861 gcatatcggt tgcgagacct cgcattgcgg cgcctgcacg gtcgatatcg gcggccgctc
     4921 ggtcaagagc tgcaccgtct tcaccgccca ggcgaatggt gccgagatca ccacgatcga
     4981 aggcatcgag ggtgcggatg gcggcctgca cgcgctgcag gagatgttcc gcgagcatca
     5041 tggcctgcaa tgcggcttct gcacgcccgg catgatcacc cgcgcctggc ggctgctcga
     5101 ggagaacccg gacccgtccg aggcggagat ccgccagggc atggccggca atctctgccg
     5161 ctgcacgggc tatcagaaca tcgtcaaggc gatccgggcg gcggccgatg cgcgccaggc
     5221 ccagcaggag gcagccgaat gaccgagcag agccccgtcg atctcgaccg cgccgagaag
     5281 ctgcagggcc tcggctgccg caggaagcgg gtcgaggatg cccgcttcac gcagggcaag
     5341 ggcaactatg tcgacgacat gaagctcaag ggcatgctgt tcggcgactt cgtccgcagc
     5401 ccctatgccc atgcccgggt gaagtcgatc gacgccgagg cggcgaaggc gctgcccggt
     5461 gtcgtcgccg tgctcaccgc cgacgatctc cggcccttga acctgcactg gatgccgacg
     5521 cttgccggcg acaagcagat ggtgctggcc gatggcaagg tcctctacca gggccaggaa
     5581 gtggcgttcg tcatcgccga gaaccgctac atcgcggcgg acgcggtcga tctcgtcgag
     5641 gtcgagtacg aggccctgcc cgtcgtcacc gacccgttca aggcgctcgc cgacgacgcg
     5701 ccggtcctgc gcgaggacat cgccgaccag aaggaagggg cgcacgggcc acgccgccat
     5761 cccaaccaca tcttcacctg ggagcagggc gacaaggcgg cgaccgaggc ggtgctcgcg
     5821 ggcgccgagg tggtggccga ggagatgatc tactaccacc gcacccaccc ctgcccgctc
     5881 gagacctgcg gctgcgtcgc gtcgatggac aaggtcaacg gcaagctgac gctttggggc
     5941 accttccagg cgccgcacgc ggtgcgcacc gtggcgtcgc tgatctccgg catcgccgag
     6001 cacaatatcc gcgtcgtctc gccggatatc ggcggcggct tcggcaacaa ggtgggcgtc
     6061 tatccgggtt atatctgcgc cgttgtcgcc tcgatcgtca ccggtgtgcc ggtgaagtgg
     6121 gtcgaggacc ggatggacaa cctgatgacc acggccttcg cccgcgacta ctggatgcag
     6181 ggcaagatcg cggcgacgaa ggacggccgg atcaccgggc tctggtgcca tgtcaccgcc
     6241 gaccacggcg ggttcgacgc ctgcgccgac ccgacgaagt tcccggccgg cttcttcaac
     6301 atctgcacag gctcctacga catcccgacg gcctggctcg cggtcgacgg cgtctacacc
     6361 aacaaggcgc cgggcggagt cgcctatcgc tgctcgttcc gggtgaccga agcctcgtat
     6421 ttcatcgagc gcatggtcga tatcctggcc atcaagctcg gcatggatcc ggtcgagctg
     6481 cggctgaaga acttcatcag gaaggaccag ttcccctacc agtcggcgct cggctgggag
     6541 tacgattcgg gcgactatca taccgccatg gagaaggcgc ttgccgccgt cgactatgcg
     6601 gggctccggc gtgagcaggc ggaacggctg gatgcgttca agcgcggcga gacgaggaag
     6661 ctcatcggca tcggcgtctc gttcttcacc gagatcgtcg gtgcggggcc ggtcaagaac
     6721 tgcgacatcc tcgggctcgg catgttcgat tcctgcgaga tccgcattca cccgacgggc
     6781 tcggccatcg cccggctcgg cacgatcagc cagggccagg gccacgccac caccttcgcg
     6841 cagatcctgg cctccgagat cggcatcccg gccgatgcga tcaccgtcga ggagggcgac
     6901 accgacacgg ccccctatgg cctcggcacc tacggctcgc gctcgacacc ggtggcgggc
     6961 gcggccaccg ccatggccgg ccgcaagatc agggccaagg cgcagatgat cgccgcctac
     7021 ctgctcgagg tgcatgacga tgacatcgag tgggacatcg accgcttcgt cgtcaaggga
     7081 gcgcccgagc gcttcaagac gatgaccgag atcgcctttg ccgcctacaa ccaggcgatc
     7141 cccgggctcg agccgggcct ggaggcggtc agctactacg acccgcccaa catgacctat
     7201 cccttcggcg cctatgtctg cgtcatggag atcgacgtcg acacgggcga gacggagatc
     7261 cgccgcttct acgccctcga cgattgcggc acgcggatca acccgatgat catcgagggt
     7321 caggtgcatg gcggcctcac cgaggcgctc gccatcgcca tgggccagga gatcgcctac
     7381 gacgagaacg gcaacgtcat gacggcgacg ctcatggact tcttcatgcc gaccgccgtc
     7441 gagacgccga cctgggagac cgatcatacg gtgaccccct cgccgcacca cccgatcggt
     7501 gcgaaggggg tcggcgagag ccccaatgtc ggcggcgtct ccgccttctc gaacgcggtc
     7561 aacgatgcgt tccggccctt cggcctgacc cacgcccaga tgccgcacga ccactggcgc
     7621 atctggcgga cggcgcaaca gctcggcctg cacggctagg ggaaaggacg aaaagcgtca
     7681 gggcatggat cacctcgacc tgcagcggcg gctggccgaa accggctatg tcgccggcga
     7741 gccgctcgcc atggcgctcc atctctccct cgtcctcggc cggccgctgc tgctcgaggg
     7801 cgaggccggg gtcggcaaga cggagatcgc caaggcactg gcgacgatcc agaagacccg
     7861 cctgatccgc ctgcagtgct acgagggtct ggacgcggcg gccgccatct acgagtggaa
     7921 ctaccagcgc cagcttctgg cgatccgtgc cgccgccgag accgcgcggg tcgaggaggc
     7981 gagcgaggag cggctcttca gcgagcgcta tctgctgcgc cggcccttgc tcgaggccat
     8041 cctgcagccg gtgccgccgg tcctgctgat cgacgagatc gaccgcgccg acgaggcctt
     8101 cgaggccttt ctgctcgaga tcctcgccga tttccagatc accgtgccgg agctcggcac
     8161 catcgaggcg gtgagccgac cgcatgtggt gctcacctcg aacgccacgc gcgagctctc
     8221 ggacgcgctt cgccgccgct gcctctacgc ctttgtcgac taccccgacc cggccatgga
     8281 gctcgccatc ctggccgccc gcgcacccga gctggagccg gcgctggcgg cgcagatcgt
     8341 cggcttcgtc caggggctgc gccgcgagga gctggagaag acgccgggca tcgccgagac
     8401 cctggactgg gtggccgcgc tttcgggcct cggcatcaac gacctcgccg ccagcctcgc
     8461 aaccgtccag gcgagcctcg tctgcctctt gaagaccagc gccgaccagg ccgccgtgac
     8521 gcccgaggtc attgcccgcc tcgtcgaccg ggccgcctga ggctcacgcc atgcagccga
     8581 cccgcttcgc cgcgcgcgcc cggggtccgg cagagcggct cgccggcttc atggcgcatc
     8641 tgcgcctgaa cggcatcgcc gtcggtccgg ccgaaaccac ccgtgctctg gccgggctcg
     8701 ccgccatcga cgccaccgat ctcggccggg cccgcctcgc cctgaagacc ctggtcacca
     8761 ccacgcccga ggactggaac cgcttcgacg agctcttcga cgcctactgg ctgaataccg
     8821 gccgcgtgcg gacccgggtc accggcagcc cgccggccgg gcagaagccg ccggccctct
     8881 ggtcacggca tctgccgcaa gccgaggggg cagcaccggt cacgggtgcg ccgaccgatg
     8941 ccgcgacggg cgaggatgac gatccggccg atggccgggg tgaaggccgg ctcgtcgcga
     9001 cgggccagcc tgcaacggcc cggcgcgacc tccgcagcct gatggcggag gccgacctgg
     9061 cggaagccga gacaatcgcc gagcggctcg cccgggccat ccgcgagcgc catgcaaggc
     9121 ggcgccgaca ggcccggctc ggccccgaga tcgatcttcg ccgcacgctg cgccgctcgc
     9181 tcgcgcgcgg cggcgagccc ctcgatctcg cgcgccgacg gcggcgggag ccacccttgc
     9241 gcatcgtcgc cctgctcgac gtctcgggct cgatgaccgt ccatgcccgc gtgttcctcg
     9301 ccttcctcaa gggcctggtc ggggccgaca gccgggccga cgcctatctc ttccacacca
     9361 gcctcgtgcg ggtgacggaa gccctcaccg cgcgggaccc gctcacggcg gccacccgcc
     9421 tctcgctcct gacccggggc atgggcggcg gcacgcggat cggccagtcg ctcgcgacct
     9481 tcaatgccac ctatgccaag cgcgcggtga acggccggac cgctgtgctc atcctctccg
     9541 acggctacga caccgacccg cccgagctgc tcgccgaggc gctcgctcgc ctccgccgcc
     9601 gcgcccggcg cgtcgtctgg ctcaacccgc tcaagggctg gcgcggctac gcgcccgtgg
     9661 cccgcggcat ggcggccgcc atgccgcatc tcgacgcctt cctcgaggcc agcacgctgg
     9721 cgagcctcgc ggccctcgaa gcgcagtttc gggagctctg agcgcatggc cgccaccgac
     9781 gtcttcgacg tgatcgagcg gctgcgcgcc acggggcggc cgttctgcgt cgcgacggtg
     9841 gtgcgcacgg ccgacgtcac ctccgccaag gcgggcgcca aggcagtggt gaccgagacg
     9901 ggcgagctga tcggccacct cggcggcgcc tgcgtcgccg gcgccgtcgc gagggcggcg
     9961 agatcggcgc tcgaggaagg tgccgtccgc ctgatccggg tcaagcccgc ggccaccgtc
    10021 gccggcgccg tcgatccgga cggggccgag ctcttcaaga gcggctgccc ttccggcggc
    10081 acggtggatc tgctgatcga gccttacgcc ctgccgccgc tgatcgcgat cctcggccgt
    10141 tcgccggtgg ccaaggccct tgcgacccag gctcgactgc taggctatcg cgtcgccgcc
    10201 gaggtgtccg gagattcggc gagcgaccgt ttcatcgaga atttcgacct gacgccactg
    10261 gcgttgcgcc cgaccgattt cgtcgtcgtc gccgcccagg gccgcggtga tctccaagcc
    10321 ctgcgcgcag cgcttggcgc acctcccgtc tatgtcggca tggtggccag ccggaagaag
    10381 gcggcggtgc tgctcgaccg cctgcgcagc gaaggcatgg acgaggcccg tctcgcccgg
    10441 ctggactcgc cggccgggct cgatctcggc ggggtcgacc cggaggagat cgcgcttgcc
    10501 atcatggcgg cgatcgtccg catcaggaac cgcggccgtc gcgccaccgg tccaagcgga
    10561 cccgaaacag ccgggggcgg gccggatgca gcgggcaagg aagtcgatcc aaccccttga
    10621 agaagatggc atccccaacg ggaatcgaac ccgtgtcttc accttgaaag ggtgatgtcc
//
LOCUS       DAOTFI010000174        10673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7358_length_10673_cov_5.936146, whole genome shotgun sequence.
ACCESSION   DAOTFI010000174 DAOTFI010000000
VERSION     DAOTFI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7358_length_10673_cov_5.936146"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="P5558_16535"
     CDS             <1..746
                     /locus_tag="P5558_16535"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRY25978.1"
                     /translation="DGAVVIQGTDTIEESAFILDLLVRGDQPVVVTGAMRGADAPGAD
                     GPANLLSAVRVAAAPEARGLGTLAVLNYDIHAARFVQKSHTALPSAFLSPLVGPIGTM
                     IEGRPRFYVTPRRNPCLPHDVGAPAPVALLKCPMGNDTRLLGGLAGLGYAGAVIEGMG
                     AGHVHADAAPVLGELARAMPVVLASRVMTGPVFTRTYGYPGAEIDLLDRGLLAAGYLS
                     GLKARLLLALALRGGTDRKALEAAFAPYA"
     gene            813..1334
                     /locus_tag="P5558_16540"
     CDS             813..1334
                     /locus_tag="P5558_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HRY25979.1"
                     /translation="MADLLVIEYPTEERAEEVRQRLLSLQTEYLIQLGDAVVAIKKPD
                     GTVKLKQLINPVKSGALGGVLWGSLIGLLFMMPLAGAAVGAAGGALGGKLTDFGINDQ
                     FMKEAAQSLHSGNAALFLLIRKMTTDKVMAELRGSGGKIMRSSFDDAKEAELRAALQE
                     AQLAIQDATPAQG"
     gene            1429..2535
                     /locus_tag="P5558_16545"
     CDS             1429..2535
                     /locus_tag="P5558_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY25980.1"
                     /translation="MPILGRLLGADSLRIGPLLAIVALGLAACDSEPEAVEPVLRPVR
                     VVTVEEREASGTVVLTGTVEATDEAALAFRIGGRMVERLVERGDTVEPGELVARLDAR
                     NEENALRAAEANLVAAEGQLVQARNHFQRQQHLMDRGFAARAVYDDAKQGYQTARSQV
                     EAAEAQRRFALDQVGFTELDADAEGVVTEIGAEPGEVVQAGQMIVGLAREGGRDAVFD
                     VPAQVLRTAPGDPVIAIVLSDDPSVTATGRVREVAPRADPVTRTFEVKVGIIDPPEEM
                     RLGASVSGRMNLEAGAMIQIPAAALTSEGGEAAVWVVEPESLTVSLRPIEVASFGPAK
                     VGIYSGLEPGEVVVVAGVQALHPGQQVRLLDAVP"
     gene            2532..5618
                     /locus_tag="P5558_16550"
     CDS             2532..5618
                     /locus_tag="P5558_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY25981.1"
                     /translation="MSGFNLSGWALRNRSLVLFLMILSVVGGIFAFVQLGRNEDPPFT
                     IRTMVVQAVWPGATLDETLLQVAERIERKLQEMEGVDVLRSYTKDGVTTVFVDLLDTV
                     PADQVPTAWQRVRNDVYDIWRTMPQGVRGPFFNDRFGDVFGIIYGFTADGFTHRELRD
                     YVEDIRSELLQVPDVSMVEILGAQDERIYVEFSMQQLATLGIDRRALLAALAAQNAVR
                     PAGEIITGDEAIRLRVSGAFDTVQDILDVNFVASDGRVLPLRDVAEVHRGFADPPQPM
                     FRVGGEPGIGLAIAMHDGGDILALGENIRATMTRLTADLPLGIEPHLVADQPVVVDQA
                     ISEFMESLWQAIAIILGMSFLSLGVRPGLVVALAIPITLAVALAIMLASGIDMQRISL
                     GALIIALALLVDDAMTTTDATLNRLGAGDDKVTAAAYPFVQYAFPMLAGTLVTIAGFV
                     PVGFAKSSAGEYTFSLFAVVTIALLVSWFVALVFTPLLNTAILKYPKPKAPDAPEPFV
                     ARTFRSLLTGAIRARWLTLGATIGAFVLAVLAFPLIPQQFFPSSDRPELLVDLRLPQG
                     ASIYATEDVAKRFDAVLEGDPDVASWSTYVGRGAIRFYLPLDVQLANDFFAQAVIVAT
                     DTEARERLQERLGKVLTEDFPDLVARLSPLELGPPVGWPLQYRVMGPDPEELRRIALE
                     LAQVISTHPQTHQTNFDWIEPVRQLRIDIDQDEARRLGLTSADVAEILATMITGTTVT
                     QVRDHIYLIDVVARATSEERVSLEALHTVQVGLPSGRTVPLGQFARFAYTQDLPLVWR
                     RDRVPTLTVQADLDAGILPAAAVAELEPAVAELARGLPRGYRIEVGGTVEESAESQAS
                     VAAVVPAMIFITLTVLMFQLRSFQRLFMALSVAPLGLIGVVAALLGSGRPMGFVAILG
                     ILALFGIITKNAVILMGEIESNRAAGKSVRDAVIDASSSRFRPIMLTALSTILGMLPI
                     AFTVFWGPMAVAIMGGLLVGTVLTLIFLPTLYCTWFELREGRAARSTSGGSTAPAT"
     gene            complement(5704..6747)
                     /locus_tag="P5558_16555"
     CDS             complement(5704..6747)
                     /locus_tag="P5558_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY25982.1"
                     /translation="MTKTAPAAGKKRLNLGLQGGGSHGAFTWGVLDRLLEVDELELVG
                     ISGTSAGAMNAAALAYGLAVGGKAGARACLAEFWGRIAAAARLSPLQPSPLDRWLGLG
                     NMDFSPGWIASDNLARLASPYELNPLNFHPLKDVLEAVVDFQALRRSCAAEQVLLFLC
                     ATNVGTGRIKIFEGEEIGLDAVLASACLPWLFQAVEIDGQHYWDGGYSGNPPIYPLIY
                     GTDCEDVLIVQINPINIESVPRTASEIVDRVNELSFNSTLMREMRAIHFVSKLLDEHR
                     VERGRYKRLNIHTVEAETELARLGSSSKVNADKDFLGWLFDLGRERCARFLDAHYEKI
                     GVESSTDVGAKFL"
     gene            complement(6752..7648)
                     /locus_tag="P5558_16560"
     CDS             complement(6752..7648)
                     /locus_tag="P5558_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25983.1"
                     /translation="MVSAYSLKRNTARPEAFEVRYLHTRDFPELLGREGQLYLRGGRE
                     VPWRNDDLQSFTPLRFAPPEMMGYRDRALLVDPDVFAVGDVMELLTRDMQGKALMCRP
                     RPLRRDGKIGDLATSVMLMDCSKLTHWQLARDFGQLFTRERDYAKWIGLKLEPEETIG
                     FFENEWNDFDRLTAETKLLHNTKRQTQPWKTGLPQDFVMADKFRWFPPKGWLRRGRQM
                     LFGYHDYGGAYMRHPDQNQENFFFGLVKECLEKGVFDEALLREEMAKNHIRHDALEVV
                     ARVPDLESWFPQGLRAPERAAA"
     gene            7865..8794
                     /locus_tag="P5558_16565"
     CDS             7865..8794
                     /locus_tag="P5558_16565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY25984.1"
                     /translation="MQLTPTAASPLAIDDALLWRLFEPFTDRSAGEVAWTREARRRKW
                     KLSKNYLRRRLLGRLTGDERSEQVILDEYEKSWSGTEYDRYSLDRFPRNDFIPWVWRD
                     RRFFATDVGGTRFRQLMLIRAIELTRPRSVLEIGCGNGINLILLACRFPEVSFTGLEL
                     TEAGQRAARGFQERHATLPDFLCDFAPEPLPDPLGFKRIRFERGSAAELPFADGSFDF
                     VQTVLALEQMEQIRARALGEMARVTAGHSFNIEPFRDVNTGWSRLYVERRNYFRGGID
                     ELSSLGLEPQWAAADFPQELFLKAAAVLSKKRG"
     gene            8808..9656
                     /locus_tag="P5558_16570"
     CDS             8808..9656
                     /locus_tag="P5558_16570"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRY25985.1"
                     /translation="MRERLRDWWLTLKDSRLTAERVKSFATADVLVVSYTKSGRTWLR
                     VLLSNYYSRRFGLSDKELIDGGNFHRQRADIPKIFFAPDTRFPYPELGPPKVHARAHQ
                     KIVFLVRDPRDVAVSLYFHVRSRAGERELRRKKIPLGARELDIDSFVLDPSFGAARMI
                     GYLNRWAEERRTLAQAHLLRYEDLRADTEATFADLLAFIGETVDPAQVSETVRFASFQ
                     SLQQKEREGYFNSDRLGQTRAGDAETAKVREGRVGGYRSSLRPETADRLDRLVEERLA
                     PDFGYR"
     gene            complement(9714..10538)
                     /locus_tag="P5558_16575"
     CDS             complement(9714..10538)
                     /locus_tag="P5558_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY25986.1"
                     /translation="MATAIEPAPAPAAPVGASRNGTGMFNYDHLELRYDPFPIGLAKP
                     LVEADRYAEMLANYPDQELFAYLAKVGHKYSLSEKSNPKAYHDFVQSKPIWRDFHAMI
                     KSDAFVKGVVDALAERHVDLGYGKLPTPARRTKKFVRNLLRGRLDAGRSPLSARFEYS
                     MLPADGGYVIPHTDSPGKVITMVVSMVGEGEWDQAIGGGTDLNKPKDPALTYNELNRQ
                     ARFDEMDVIDTFEFTPNQGVIFVKTFNSWHSVRPMTGKGSKLMRKTLTINIESYDN"
BASE COUNT         1580 a         3870 c         3499 g         1724 t
ORIGIN      
        1 tcgacggtgc cgtggtcatc cagggcaccg acacgatcga ggaatcggcc ttcatcctcg
       61 atctcctggt ccggggcgac cagccggtgg tggtcaccgg ggccatgcgc ggcgccgacg
      121 cgccgggcgc cgacgggccg gccaacctgc tctccgccgt gcgggtcgcc gccgcgcccg
      181 aggccagggg cctcggcacc ctcgccgtgc tcaactacga catccacgcc gcccgcttcg
      241 tccagaaatc gcatacagcc ctgccatcgg ccttcctctc gccgctggtc ggacccatcg
      301 gcacgatgat cgagggtcgc ccgcgtttct acgtcacccc gagacgcaac ccgtgcctgc
      361 cgcacgacgt cggcgcgccg gcgcccgtcg ccctcctcaa atgcccgatg ggcaacgaca
      421 cgcggctcct cggcggcctg gccggcctcg gctacgccgg tgcggtcatc gaaggcatgg
      481 gcgccggcca cgtccacgcg gatgccgcac cggtgctcgg cgagctggcc agggcgatgc
      541 cggtcgtcct cgcgtcccgc gtcatgaccg gcccggtctt cacccgcacc tacggctatc
      601 cgggtgccga gatcgacctg ctcgaccgcg gcctcctcgc ggccggctat ctctccggcc
      661 tcaaggcccg tttgctgctg gccctggcgc tgcgcggcgg gaccgaccgc aaggcgctcg
      721 aagcggcgtt cgcaccctat gcctgagatg gcggacgcga gcttctgctg ctatcagcac
      781 acccgaaacg atccatcgag gagcaggaca ccatggcgga ccttttggtc atcgagtacc
      841 cgaccgagga gcgggccgag gaggtgcggc agcgcctcct gagcctgcag acggagtatc
      901 tgatccagct cggcgacgcc gtcgtcgcga tcaagaagcc cgacgggacg gtcaagctca
      961 agcagctcat caacccggtg aaatccggcg cgctcggtgg cgtgctctgg ggctcgctca
     1021 tcggcctgct cttcatgatg ccgctcgccg gcgccgcggt cggtgctgcc ggcggcgcgc
     1081 tcggcggcaa gctcaccgac ttcggcatca acgaccagtt catgaaggaa gcggcgcaga
     1141 gcctgcattc cggcaacgcc gccctcttcc tcctgattcg caagatgacc accgacaagg
     1201 tcatggcgga gctgcggggc agcggcggca agatcatgcg cagctcgttc gacgacgcca
     1261 aggaggcgga gctgcgcgcg gcgctccagg aggcgcagct cgccatccag gatgccactc
     1321 cggcgcaggg ctgacgcacc gtcgaccatg catcatcgcc cgccccggcc cgccggccgg
     1381 ggcggaatct cgtgccattc gctctctgag accttcgcga gacctgacat gccaatactc
     1441 ggtcggcttc tcggtgccga tagcctgcgg atcggcccct tgctggccat cgtcgctctc
     1501 gggctcgccg cctgcgacag cgagcccgag gcggtcgaac cggtgctccg accggtccgc
     1561 gtcgtgaccg tcgaggagcg cgaggcgtcc ggcaccgtgg tgctgaccgg caccgtcgag
     1621 gccaccgacg aggcggcgct cgcctttcgc atcggcgggc gaatggtcga gcggctggtc
     1681 gagcggggcg ataccgtcga gcccggcgaa ttggtggccc ggctcgatgc ccgcaacgag
     1741 gagaacgcgc tccgcgccgc cgaggcgaat ctcgtcgccg ccgaaggcca gctcgtccag
     1801 gcccgcaatc atttccagcg ccagcagcac ctgatggacc gcggcttcgc cgcgcgcgcc
     1861 gtctacgacg acgcgaagca gggctaccag acggcccgct cgcaggtcga ggcggccgag
     1921 gcgcagcggc gcttcgccct ggaccaggtg ggcttcaccg agctcgacgc cgacgccgag
     1981 ggcgtggtga ccgagatcgg cgccgagccc ggcgaggtcg tgcaggccgg ccagatgatc
     2041 gtcgggctcg cccgcgaagg cgggcgcgac gcggtcttcg acgtgccggc ccaggtgctg
     2101 cgcacggcac ccggcgatcc ggtcatcgcc atcgtgctca gcgacgaccc ttcggtcacc
     2161 gccaccgggc gggtccgcga ggtggcgccg cgcgccgacc cggtgacccg caccttcgag
     2221 gtcaaggtcg gcatcatcga cccgcccgag gagatgcgcc tcggcgccag cgtcagcggg
     2281 cgcatgaacc tcgaggccgg cgcgatgatc cagataccgg cggccgccct caccagcgag
     2341 ggcggcgagg cggccgtctg ggtggtcgag cctgagagcc tgacggtgag cctcagaccc
     2401 atcgaggtcg cctcgttcgg cccggccaag gtcggcatct atagcgggct cgagcccggc
     2461 gaggtggtcg tcgtggccgg cgtccaggcg ctccatccgg ggcagcaggt ccgcctcctg
     2521 gacgccgtgc cttgagcggc ttcaatctct cgggctgggc gctccgcaac cgctcgctcg
     2581 tcctcttctt gatgatcctc tcggtcgtcg gcggcatctt cgccttcgtt cagctcggcc
     2641 gcaacgagga tccgcccttc accatccgca ccatggtcgt ccaggccgtc tggccgggcg
     2701 ccacgctcga cgagaccctg ctgcaggtgg ccgagcgtat cgagcgcaag ctgcaggaga
     2761 tggagggcgt cgacgtcctg cgcagctaca cgaaggacgg ggtgaccacc gtcttcgtcg
     2821 acctgctgga cacggtgccc gccgaccagg tgccgaccgc ctggcagcgc gtgcgcaacg
     2881 acgtctacga catctggcgg accatgccgc agggggtccg cggccccttc ttcaacgacc
     2941 ggttcggcga cgtcttcggc atcatctacg gcttcaccgc cgacggcttc acccatcgcg
     3001 agctgcgcga ctacgtcgag gacatccgct ccgagctcct gcaggtgccg gacgtctcga
     3061 tggtcgagat cctgggcgcc caggacgagc ggatctacgt cgagttctcg atgcagcaac
     3121 tggccaccct cggcatcgac cgccgggccc tgctcgcggc cctcgccgcc cagaacgcgg
     3181 tccgcccggc cggcgagatc atcaccggcg acgaggccat ccggctgcgc gtctccggcg
     3241 ccttcgacac ggtccaggac atcctcgacg tcaacttcgt cgccagcgac ggccgcgtgc
     3301 tgccgctgcg ggacgtggcc gaggtgcatc gcggcttcgc cgacccgccg cagccgatgt
     3361 tccgcgtcgg cggcgagccg ggaatcggcc tcgccatcgc catgcacgac ggcggcgaca
     3421 tcctggcgct gggggagaac atccgcgcca ccatgacgcg gctcaccgcc gacctgccgc
     3481 tcggcatcga gccgcatctc gtcgccgacc agccggtggt ggtcgaccag gcgatttccg
     3541 agttcatgga atcgctttgg caggcgatcg ccatcatcct cggcatgagc ttcctcagcc
     3601 tcggcgtgcg gcccgggctg gtcgtcgccc tggcgatccc gatcaccctc gccgtggcgc
     3661 tggcgatcat gctcgcctcc ggcatcgaca tgcagcggat ctcgctcggt gccctgatca
     3721 tcgccctggc gctgctggtc gacgacgcca tgaccacgac ggacgccacc ctcaatcggc
     3781 tcggcgccgg cgacgacaag gtgaccgccg cggcctatcc cttcgtccag tacgcgttcc
     3841 cgatgctcgc cggcacgctc gtcaccatcg ccggcttcgt gccggtcggc ttcgccaaga
     3901 gctccgccgg cgaatacacc ttctcgctgt tcgccgtcgt caccatcgcg cttctggtct
     3961 cctggttcgt cgccctggtc ttcacgccgc tcctcaacac ggccatcctc aagtacccga
     4021 agcccaaggc gcccgacgca cccgagccct tcgtcgcccg caccttcagg tcgctcctga
     4081 ccggcgccat ccgggcccgc tggctgacgc tgggcgccac catcggcgcc ttcgtcctcg
     4141 ccgtcctcgc ttttcccctg atcccgcagc agttcttccc gtcctccgac cggccggagc
     4201 tcctggtcga tctgcggctg ccgcagggcg cctcgatcta tgccaccgag gacgtggcca
     4261 agcgcttcga tgccgtgctc gagggcgacc ccgacgtcgc cagctggagc acctatgtcg
     4321 gccggggtgc gatccgcttc tatctgccgc tggacgtcca gctcgccaac gacttcttcg
     4381 cccaggcggt gatcgtggcg accgacaccg aggctcgcga gcggctgcag gagcggctcg
     4441 ggaaggtgct gaccgaggac ttcccggacc tcgtcgcccg cctctcgccc ctggagctcg
     4501 gcccgccggt cggctggccg ctgcaatatc gggtgatggg cccggacccc gaggagctgc
     4561 gacgcatcgc cctcgagctc gcccaggtca tctcgaccca cccgcagacg caccagacca
     4621 atttcgactg gatcgagccc gttcgccagc tccgcatcga catcgaccag gacgaggcgc
     4681 gccggctcgg gctgacctcg gccgacgtcg ccgagatcct ggccaccatg atcaccggca
     4741 ccacggtcac ccaggtccgc gaccacatct acctcatcga cgtggtcgcc cgggcgacca
     4801 gcgaggagcg ggtctcgctc gaggcgctcc acaccgtgca agtcggcctg ccctccggcc
     4861 gcaccgtgcc gctcggccag ttcgcccgct tcgcctacac ccaggacctg ccgctcgtct
     4921 ggcgccgcga ccgggtgccg accctgaccg tccaggccga cctggacgcc ggcatcctgc
     4981 cggcggccgc cgtcgccgag ctcgagccgg ccgtcgccga gctcgcccgg ggcctgcccc
     5041 gcggctaccg catcgaggtc ggcggcaccg tcgaggaaag cgccgaatcc caggcctcgg
     5101 tggccgccgt cgtgccggcc atgatcttca tcacgctgac cgtgctgatg ttccagctca
     5161 ggagcttcca gcgtctgttc atggcgctca gcgtcgcccc gctcggcctg atcggcgtgg
     5221 tggcggcgct gctcggctcc ggccggccga tgggcttcgt cgccatcctc ggcatcctcg
     5281 ccctcttcgg catcatcacc aagaacgccg tcatcctcat gggcgagatc gaatccaatc
     5341 gcgccgccgg caagagcgtg cgcgatgctg tcatcgacgc cagcagctcg cgcttcaggc
     5401 cgatcatgct caccgccctc tccaccatcc tcggcatgct gccgatcgcc ttcaccgtct
     5461 tctgggggcc gatggccgtc gccatcatgg gcggcctcct ggtcggcacc gtcctgaccc
     5521 tcatcttcct gcccaccctc tactgcacct ggttcgagct gcgcgagggc cgcgccgcac
     5581 gctccacctc tggcgggtcg acagcgccgg cgacgtgagc ggccgcgttg atcgatgctc
     5641 tttccaatcg aagggacgtc ggggaaactg aaacccgccg ccgtcccttc gagaaccggc
     5701 ccctcaaaga aacttcgccc cgacgtccgt cgagctctcg accccgatct tttcgtagtg
     5761 cgcgtcgagg aaccgcgcgc aacgctcgcg gccgaggtcg aagagccagc cgaggaagtc
     5821 cttgtcggcg ttcaccttgg acgagctgcc gagccgggcg agctctgtct ccgcctcgac
     5881 ggtgtggatg ttgagccgct tgtagcggcc ccgctcgacg cgatgctcgt cgagcagctt
     5941 ggagacgaaa tggatggcgc gcatctcgcg catcagggtc gagttgaacg agagctcgtt
     6001 cacccggtcg acgatctcgg atgccgtcct gggcacgctt tcgatgttga tcgggttgat
     6061 ctggacgatc agcacgtcct cgcagtcggt gccgtagatc aggggataga tcggcgggtt
     6121 gccggaatag ccgccgtccc agtagtgctg cccatcgatc tcgaccgcct ggaagagcca
     6181 gggcaggcag gccgaggcga gcaccgcgtc gaggccgatc tcctcgccct cgaagatctt
     6241 gatccggccg gtgccgacat tggtggcgca gaggaagagc agcacctgct cggcggcgca
     6301 gctccgccgg agcgcttgga aatcgaccac cgcctcgagc acgtccttca gcggatggaa
     6361 gttcagcggg ttgagctcgt agggcgaggc cagccgcgcc aggttgtcgg aggcgatcca
     6421 gccgggagag aagtccatgt tgccgagccc gagccagcgg tcgagcgggc tcggctgcaa
     6481 gggcgagagc cgagccgcgg cggcgatccg cccccagaac tcggctaggc aggcgcgcgc
     6541 ccccgccttg ccgcccacgg cgaggccgta ggccaaggcc gccgcgttca tcgccccggc
     6601 gctggtgccg ctgatgccga ccagctcgag ctcgtccacc tcgagcagcc ggtcgagcac
     6661 gccccaggtg aaggcgccat gcgagccccc accctgcagg ccgagattga ggcgcttctt
     6721 gccggccgcc ggcgccgtct tcgtcaccgc ctcaggcggc cgcccgctcc ggcgccctga
     6781 gtccctgcgg gaaccagctc tcgaggtccg gcacccgcgc caccacctcg agcgcgtcgt
     6841 ggcggatgtg gttcttcgcc atttcctcgc gcaagagcgc ctcgtcgaag acgcccttct
     6901 ccaggcattc cttgaccagg ccgaagaaga agttctcctg gttctggtcg gggtgcctca
     6961 tgtaggcgcc gccatagtcg tggtagccga agagcatctg gcggccgcgg cgcagccagc
     7021 ccttgggcgg gaaccagcgg aacttgtcgg ccatgacgaa gtcctgcggc agcccggtct
     7081 tccagggctg cgtctgccgc ttggtgttgt gcagcagctt ggtctcggcg gtcagccggt
     7141 cgaaatcgtt ccactcgttc tcgaagaagc cgatcgtctc ctccggctcc agcttgagcc
     7201 cgatccactt ggcgtagtcg cgctccctgg tgaagagctg gccgaaatcc cgggccaact
     7261 gccagtgggt cagcttgctg caatccatca gcatgacgct ggtcgcgaga tcgccgatct
     7321 tgccgtcgcg gcgcaatggc cggggccggc acatcaatgc cttgccctgc atgtcgcggg
     7381 tcagaagctc catgacgtcg ccgacggcga agacatccgg gtcgaccaag agcgcccggt
     7441 cccggtagcc catcatctcg ggcggggcga agcgcagcgg cgtgaacgac tgcaggtcgt
     7501 cattgcgcca gggcacctcg cggccgcccc gcaggtagag ctggccttcg cggccgagca
     7561 gctccggaaa gtccctggtg tgcagatagc gcacctcgaa ggcttccggc cgggcggtat
     7621 tgcgcttcaa cgagtaggcc gataccaacg ctcccaccca ctggcggtgg ttggtgtgga
     7681 tgaagacagt gggtttggtg gcaactggcg cagcgctggg cgatgtcacg gcgacccccg
     7741 cggtccttgg tgaacaggtg atgagcggcg atagcaggaa tggcacggcc gcggcaagca
     7801 acacccggca gcacccgtct tgccgacaag cgtgcgcctg ccgtcctacc ggagaataga
     7861 cccaatgcag ctcacgccaa cagcggcgag cccgctcgcg atcgacgacg cgctcctgtg
     7921 gcggctcttc gagccgttca ccgatcggag cgccggcgag gtggcctgga cgcgcgaggc
     7981 ccgacggcgc aagtggaagc tttcgaagaa ctatctccgc cgcaggctgc tcggccggct
     8041 gaccggtgac gaacgcagcg agcaggtgat cctcgacgag tacgagaaat cctggtcggg
     8101 caccgagtac gaccgctaca gcctcgaccg cttcccgagg aacgacttca tcccctgggt
     8161 ctggcgggac cgccgcttct tcgccaccga cgtcggcggc acccgctttc gccagctcat
     8221 gctgatccgc gccatcgagc tcacccggcc gagaagcgtg ctggagatcg gctgcggcaa
     8281 cggcatcaac ctcatccttc tcgcctgccg cttccccgag gtcagcttca ccgggctcga
     8341 gctcaccgag gccggccagc gcgcggccag gggattccag gagcgccacg cgaccctgcc
     8401 ggacttcctc tgcgacttcg cgcccgagcc cttgccggat cccctgggct tcaagcgcat
     8461 ccgcttcgag cgcggctcgg ccgccgagct gcccttcgcc gatggcagct tcgattttgt
     8521 ccagaccgtg ctggcgctcg agcagatgga gcagatccgc gcccgtgcgc tcggcgagat
     8581 ggcccgcgtc acagccggcc acagcttcaa catcgagccc ttccgcgacg tcaacaccgg
     8641 ctggtcgcgg ctctatgtcg agcggcgcaa ctactttcgg ggcggcatcg acgagctttc
     8701 gagcctcggc ctcgagccgc aatgggcggc cgccgacttc ccgcaggagc tcttcctcaa
     8761 ggccgcggcg gtcctctcga agaagcgcgg ctgagcgggg gcggctcgtg cgggagcggt
     8821 tgcgcgactg gtggctgacg ctgaaggaca gccggctcac cgcggagcgg gtgaagagct
     8881 tcgccacagc ggacgtgctc gtcgtctcct acaccaagag cggccgcacc tggctgcgcg
     8941 tgctgctctc gaactactac agccgtcgct tcggcctctc cgacaaggag ctgatcgacg
     9001 gcggcaattt ccaccgccaa cgggccgaca taccgaagat cttcttcgcg cccgacacgc
     9061 gcttccccta tccggagctg ggcccgccca aggttcatgc cagggcgcac cagaagatcg
     9121 tcttcctggt tcgcgacccg cgcgatgtcg ccgtatcgct ctattttcac gtgaggagcc
     9181 gggccggcga gcgcgagctg cgtcgcaaga agatcccgct cggcgcccgc gagctcgata
     9241 tcgacagctt cgtgctcgac ccttccttcg gcgccgcccg gatgatcggc tatctcaatc
     9301 gctgggccga ggagcggcgc acgctcgccc aggcgcatct cctgcgctac gaggacctcc
     9361 gcgccgacac cgaagccacc ttcgccgacc tcctcgcctt catcggcgag accgtcgatc
     9421 cggcgcaggt gtccgagacc gtccgcttcg cctccttcca gagcctgcag cagaaggagc
     9481 gcgagggcta cttcaactcc gaccgcctcg gccagacgcg cgccggcgac gccgagacgg
     9541 ccaaggtccg cgagggccgg gtcggcggct atcgctcttc gctcaggccc gagaccgccg
     9601 accggctgga tcggctggtc gaggagcgcc tcgcccccga cttcggctat cgctgagcgc
     9661 tgccccgccg cttgctcatg aagctggcct cggacgcctg aggccgggag gcgctagttg
     9721 tcgtagctct cgatgttgat ggtcagcgtc ttgcgcatca gcttgctgcc cttgccggtc
     9781 atcggccgga ccgagtgcca ggaattgaac gtcttgacga agatcacgcc ctggttcggc
     9841 gtgaactcga acgtgtcgat cacgtccatc tcgtcgaacc gcgcctgccg gttcagctcg
     9901 ttgtaggtga gcgccgggtc cttcggcttg ttgaggtcgg tgccgccgcc gatcgcctgg
     9961 tcccattcgc cctcgccgac catcgagacc accatggtga tgaccttgcc cggcgaatcg
    10021 gtgtgcggga tcacgtagcc gccatccgcc ggcagcatcg agtactcgaa gcgggcggag
    10081 agcggcgacc tgccggcgtc gagccggccg cgcagcaggt tgcgcacgaa tttcttcgtc
    10141 cggcgcgccg gggtcggcag cttgccgtag ccgagatcga catggcgctc cgccagggcg
    10201 tccaccacgc ccttgacgaa ggcgtcgctc ttgatcatgg cgtgaaaatc gcgccagatc
    10261 ggcttcgact ggacgaaatc gtgataggcc ttggggttgc tcttctccga gagcgagtat
    10321 ttgtgcccga ccttggcgag ataggcgaaa agctcctggt cgggatagtt cgccagcatc
    10381 tcggcatagc ggtcggcctc gaccagcggc ttggcgaggc cgatggggaa ggggtcgtag
    10441 cgcagctcga ggtggtcgta gttgaacatg cccgttccgt tccgggaagc gccgaccggg
    10501 gcggccgggg cgggggctgg ttcgatggct gtggccatgg ctctaggagg ctccttgggc
    10561 ggccagcggc gccgcgtcgc gtcggttgcc ctgctccctc ggcggcgccg ggaggaaggg
    10621 caggccggca cgatcgtgca gcacctgaac gacacgacgg cggcgctctc ggc
//
LOCUS       DAOTFI010000175        10515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7505_length_10515_cov_5.583174, whole genome shotgun sequence.
ACCESSION   DAOTFI010000175 DAOTFI010000000
VERSION     DAOTFI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7505_length_10515_cov_5.583174"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="P5558_16580"
     CDS             <1..376
                     /locus_tag="P5558_16580"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY25987.1"
                     /translation="AEHEYTRWGFKRFVDQAALDILQPDIYWCGGLSETLKITALATT
                     HDLIVIPHGHSTPIGIHYSAAQSPIHTPYQEYLVKWNEINMHFLATPIRPRNGMIHVP
                     DGPGVGMELDPARIEAEVEVVV"
     gene            complement(520..2334)
                     /locus_tag="P5558_16585"
     CDS             complement(520..2334)
                     /locus_tag="P5558_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY25988.1"
                     /translation="MTSPGAARRLRYHTVRLRNRPDLVIGYVCLVALGFLVLGPLVEI
                     VRDALTLQPYDLAYHPDGVVGSFSLFHLERVFASPLSPALFFRPLLNSLAVGFWVTAI
                     GVSIGSLLAWLLVRTDIRCKGLLGALIVIPYMMPSWVMALAWISLFKNDRIGGTQGFV
                     ASFLGVQPPDWVSYGFFPIVVTLALHYFAYAYLLMSGALATVDSELEEAGAVCGMGRW
                     KRLWRITLPLLMPALGSAIVLTFIRILGTFGTPALLGLPVRFFTFATQIYASINARNN
                     GDAFVLALVLIVIAISFIWINSRIIGIRKSFVTLTGKGFRRREIRLGLWRLPLTAAVG
                     LFVASTVLVPLSILLWESLILVAGDYSLDNLTTHFWIGQGNVRLAYGEAGVLLNNGIL
                     TALWNSLKLATLAALFNGVLGLLVGYAVVRGRGTRLSRGLEGIAFAPYIFPSIALGAI
                     YIGMFSAPIGPIPALYGTFAILVLITVVKNLPFTSRTGIAAMLQIDTSMEESARVLGI
                     GWFRRMARIIMPLSMSGLVSGMLLTFITAMRELSLIVLLLSPANMVLTGVIFYYQEQS
                     MPQHSSAVTLLLVLIIILVNVLVRLVSRGAGVSGLKNS"
     gene            complement(2356..3453)
                     /locus_tag="P5558_16590"
     CDS             complement(2356..3453)
                     /locus_tag="P5558_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY25989.1"
                     /translation="MAQIGLSGIRKAYGREVVVHDLDLTIRPGEFLTILGPSGCGKTT
                     TLRAIAGLEEPDDGDIHLGEKLVFSRRRGVIVPPEKRGVGLIFQSYALWPHMTVQRNV
                     SLALEEKGLGRAEIEARATQALETVQMTALGARYPSELSGGQQQRVAVARMIALRPQI
                     LLMDEPLSNLDAKLRLDMRAELLQLHRDLGSTTVYVTHDQAEALTLSDRIVVMDRGRV
                     MQEASPYEVYHHPRSLQVAEFIGEPRINLVPGTLAREAGTLYLVGDGVRLPAERARAA
                     KGERLVAAVRPENIELSTVPVAGWQEVRLDNVQPTGATTILQVCAGATRLRLVRPGFL
                     RIEAGIPLWIGFDADSIEFFDPDTQGNVSAP"
     gene            complement(3463..4713)
                     /locus_tag="P5558_16595"
     CDS             complement(3463..4713)
                     /locus_tag="P5558_16595"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY25990.1"
                     /translation="MTRSARYWRATAGALALAWLAALPVAATAQEVSPETEAWLKAAE
                     LGAHAPATEGWDAIVAKAQEEGEVVVYSSSGRMAMLVDAFARTYPGIVLTVFDLGSVK
                     TVEKTIREQQAGIFNADVVSTGNSGAVIHEMLNQGLIFNYVPEAYKSRIPEENREPLL
                     IRINEAIVIYYNKEAYPDAPPATNIWELTEPEFNGRVGIIDPMSSGSSFMGLATIVQH
                     ADEMAAAYKRHTGHDIELGEGVPDAGYEFVARLLQNDVVIFKSGSSLVEASGQPGQEK
                     PLLAFAYMTYISKNESDGFVNAYLADLDPVAKWVYPTFTAIANRAPHPNAAKVFTAYL
                     LGSLELNESSVLEKPFTEGRSMELLQGLAPYYDPGSVSPRNDVPLPPGGEAWGEMKGW
                     TVSADFMQSEGPRLRDFWLLHASQ"
     gene            complement(4816..5559)
                     /locus_tag="P5558_16600"
     CDS             complement(4816..5559)
                     /locus_tag="P5558_16600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY25991.1"
                     /translation="MADARAGRGAGFSRIRLAHVELVIFDCDGVLIDSESIASLALSE
                     TLGEFGVVMTPDEAHARFTGGAEEDTRAHCRELLGTTALDEFFALYAKRLYGGFRNLR
                     EVEGIGDVVRALDCPRCVASNSSLYRLTRSLGRLDLWQAFAGQVFSADLVARPKPAPD
                     LPLLCAAEFGAHPARCVVIDDSPHGIEAAVAAGMLAIGFVEPGDPRPSRPDVLRRHGA
                     RFVAEGSDALRALLPEADAWLGERRRDPG"
     gene            complement(5552..6304)
                     /locus_tag="P5558_16605"
     CDS             complement(5552..6304)
                     /locus_tag="P5558_16605"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRY25992.1"
                     /translation="MSIVVQIVGHRGGRNVWPENSLLGFRKVLEMAVDAVEFDVHLTK
                     AGELLVIHDPTLERTTTGTGKVAELAPCAHREVVLRGSEGETIPTLAEVLEVFRDARI
                     DLHVELKSDGDHVPYPGIVEAVVAVLAGFDFGERAMLTSFDPDVLGLIRDALPEGRRL
                     CSIDRESAARLGGLETAVRHFEPLVEVVAIQKDLMREHWDAITALVEPGRLAAWTVND
                     EAEIDHWLRQPIRSITTDRPDLAVAAKERLHG"
     gene            complement(6324..7241)
                     /locus_tag="P5558_16610"
     CDS             complement(6324..7241)
                     /locus_tag="P5558_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY25993.1"
                     /translation="MNQDRTLNIVCHAILVVGAVLVCLPLYFAFVAGSLTIVEVQQTP
                     FPLLPGGHFFENLAESWSRGNFGRLFVNSIIITGGIVIGKLVMSLLAAYAITYFRFPF
                     RMTAFWLIFVSLMLPVEVRIIPTYEAVADAAGPLRWLAGVTGLSGLVETLTGYRLETA
                     LQWNMVNTYRGLILPLIASASATFLFRQFFLTVPEELCEAARLDGAGPVKFFFDILLP
                     LSKVNIAALAIILFIYGWNQYLWPLLFTTDKDMATAVLGLKQLVPVSDAEPAWNLAMA
                     ATFVVLLPPAIIIIGLQGLFTRGLVDSGK"
     gene            complement(7252..8247)
                     /locus_tag="P5558_16615"
     CDS             complement(7252..8247)
                     /locus_tag="P5558_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY25994.1"
                     /translation="MPAADRTATTGRGAPGRPFFFGIGRLLKLDGRRSASGLKKAHFT
                     QPWFAAGLLAPQLLILLFFFFIPSYKALSLAFVQIDPFGGRVLFVGFDNFRQLFGSAQ
                     YRYSAAFTLIFTAVVSIGTLLIALVFAFATDFVIRGRGLYKSVILLPYAIAPVISGVL
                     WAFLFNPAVGPLAQLMHALGLEWDPNRRPMDAQILIIVAAIWKQICYNYIFLVASLLG
                     VPQSLLEAATLDGARPIRRFFTVSLPLVMPVVFFLMVMNFIYALFETFGIVDAVTRGG
                     PNGATNILVYKVFQDGFVLLDLGSSAAQSVVLMIVAVSFTVLQFRAIERKVNYQV"
     gene            complement(8234..9535)
                     /locus_tag="P5558_16620"
     CDS             complement(8234..9535)
                     /locus_tag="P5558_16620"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY25995.1"
                     /translation="MLALTVAMLPLAGGELAAETIKAEWWHAMRGRLTPIVEEMIANF
                     NAGQDKYEIVGVLKGNYEETAAAMVAAYRVNEHPTLVQTSERAFLTMYNSGAIVPVPE
                     LMAREGYDIDWDNFIAPVAGFYLVDGKPAAMPFNSSTPIFWYNADNFEAAGFPDGPAE
                     TWQEVEKQLYAIKEQGISECPMSLPNDFVWSLIENYSAIHDQEFGTKANGFGGLDTEF
                     TFNQSPVIVAQLERLQKWMNDGVLHIAGQGLNPEALFISGDCSTITASTAMHAAVESG
                     AQFDWGATFMPHEEGMEPMNSTIGGGALWVLQNKSEEEYEAIAAFLNFVASPETQAWW
                     SEQTGYVPITNAAYDMMKGEGYFEAHPTREIAIVQLNRGTPSDNSRGFRFGNHNQIFA
                     ITNEEIQGVWTGQSTPQQALDNSAARGNEVLRQFERLNAGG"
     gene            9773..>10515
                     /gene="ugpC"
                     /locus_tag="P5558_16625"
     CDS             9773..>10515
                     /gene="ugpC"
                     /locus_tag="P5558_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY25996.1"
                     /translation="MADIHLNDLRKSYGTVEAVKGVSLAVQDGEMIVLVGPSGCGKST
                     LLRMIAGLESITARELHIGGRDVSRVEPAERDIAMVFQNYALYPHMSVRQNLSYGLRN
                     RGVPRQEIALRVDDVARILELGMLLDRRPGQLSGGQRQRVAMGRAIVRNPAAFLFDEP
                     LSNLDARLRVQMRVEIRKLQRRLRTTSLYVTHDQLEAMTLADRLVVMNAGEIEQIGTP
                     LEVYVRPETLFVAGFIGSPPMNLLPLDILA"
BASE COUNT         1838 a         3613 c         3488 g         1576 t
ORIGIN      
        1 tgccgagcac gagtacacgc gctggggctt caagcgcttc gtcgaccagg cggcgctcga
       61 tatcctgcag cccgacatct actggtgcgg cggcctctcc gagacgctga agatcacggc
      121 gctcgccacc acccacgacc tgatcgtcat cccgcacggc cactcgacgc cgatcggcat
      181 ccactactcc gccgcccagt cgccgatcca cacgccctac caggaatacc tagtcaaatg
      241 gaacgagatc aacatgcact tcctggccac ccccatccgc ccgaggaacg gcatgatcca
      301 cgtccccgac ggccccggcg tcggcatgga gctggacccg gcgaggatcg aggcggaggt
      361 cgaggtcgtt gtctgaggaa agcggcctcg gcggcagcgg cggccggccc atcctcgagc
      421 ggatgctccg cctcgaggag aagctgaagg cgggcggcca cgtcaatgcg cctggctctc
      481 cccgcccgac ccgaccgtgg ccatttgtcg gccagactcc tagctgttct tcagcccgct
      541 gacgccggcg ccccttgaga cgaggcgcac gagcacgttc acgaggatga tgatcagcac
      601 gagcagcagc gtcaccgcgc tggaatgctg cggcatgctc tgctcctgat agtagaagat
      661 cacgccggtc aggaccatgt tggcgggcga gagcagcagc acgatgagcg acagctcgcg
      721 catcgccgtg atgaaggtca gcagcatccc cgagacgagg ccgctcatcg acaggggcat
      781 gatgatgcgc gccatgcggc ggaaccagcc gatgccgagg acccgcgccg actcctccat
      841 cgacgtgtcg atctgcagca tcgccgcgat gccggtgcgc gacgtgaacg gcaggttctt
      901 gaccaccgtg atcagcacca ggatggcgaa ggtgccgtag agcgccggga tcggccctat
      961 cggcgcggag aacatgccaa tatagatggc acccagcgct atcgacggga agatgtaggg
     1021 ggcgaaggcg atgccctcca gccccctcga caggcgcgtg ccgcgcccgc gcaccacggc
     1081 gtagccgacc agcaggccga gcacgccgtt gaacagggca gccagggtgg cgagcttcag
     1141 gctgttccac agcgccgtca ggatgccgtt gttcaggagc acgccggcct cgccataggc
     1201 cagccgcaca ttgccctgcc cgatccagaa atgggtcgtc aggttgtcca ggctgtagtc
     1261 gccggcgacc aggatcaggg attcccagag caggatcgac agcggcacga gcacggtcga
     1321 ggcgacgaac aggccgaccg ccgcggtcag cggcaggcgc cacaggccga gccggatctc
     1381 gcgccggcgg aagcccttgc cggtcagcgt cacgaagctc ttgcggatgc cgatgatgcg
     1441 gctgttgatc cagatgaagc tgatggcgat gacgatcagc accagcgcca ggacgaaggc
     1501 gtcgccattg ttgcgggcgt tgatcgaggc gtagatctgc gtcgcgaagg tgaagaagcg
     1561 caccggcagg ccgagcaggg ccggcgtgcc gaaggtgccg aggatgcgga tgaaggtcag
     1621 cacgatggcc gagccgagcg ccggcatcag cagcggcagg gtgatgcgcc agagccgctt
     1681 ccagcggccc atgccgcaga ccgccccggc ctcctccagc tcggaatcga ccgtcgccag
     1741 ggcacccgac atcaaaaggt aggcataggc gaagtagtgc agcgccagcg tcaccacgat
     1801 ggggaagaag ccgtaggaga cccagtccgg cggctggacg ccgaggaagc tcgcgacgaa
     1861 gccctgcgtg ccgccgatgc ggtcgttctt gaacaggctg atccaggcca gcgccatcac
     1921 ccatgacggc atcatgtacg ggatgacgat cagggccccg agcaggccct tgcagcggat
     1981 gtccgtgcgc acgagcagcc aggccagcag cgagccgatg ctgacgccga tggcggtcac
     2041 ccagaagccg acggccaggc tgttcagcag cgggcggaag aacagcgccg gcgagagcgg
     2101 gctggcgaac acccgctcca ggtggaagag cgagaacgac cccaccacac cgtcggggtg
     2161 ataggccagg tcgtagggct gcagggtcag cgcgtcgcgg acgatctcga ccagcggccc
     2221 cagcaccaaa aagccgagcg ccacgaggca gacatagccg atgacgaggt cgggccggtt
     2281 gcggagccga accgtgtgat agcgcagccg gcgcgccgcc cccggggaag tcatgctcgc
     2341 cacattcccg cggcgctacg gcgccgacac gttgccctgg gtgtccggat cgaagaactc
     2401 gatgctgtcc gcatcgaagc cgatccagag cgggatgccc gcctcgatgc gcaggaagcc
     2461 cggccgcacc agccgcagcc gggtggcccc ggcgcaaacc tgcaggatgg tggtggcccc
     2521 cgtcggctgg acattgtcga ggcgcacctc ctgccagccc gccacgggca ccgtcgacag
     2581 ctcgatattt tccggccgga cggcggcgac cagccgctcg cccttcgccg cccgcgcccg
     2641 ttccgccggc agccggacgc cgtcgccgac cagatagagc gtgccggcct cccgcgcgag
     2701 cgtgccggga acgaggttga tccggggctc gccgatgaac tcggccacct ggaggctcct
     2761 gggatggtga tagacctcgt agggcgacgc ctcctgcatg acccgcccgc gatccatgac
     2821 gacgatgcgg tccgagaggg tgagggcctc ggcctggtcg tgggtgacat agacggtcgt
     2881 cgatccgagg tcccggtgca gctgcagcag ctccgcccgc atgtccagcc gcagcttggc
     2941 atcgaggttg gacaagggct cgtccatcag caggatctgg gggcgcagcg cgatcatccg
     3001 tgctaccgcc acccgctgct gctggccgcc ggaaagctcc gacggatagc gcgccccgag
     3061 cgccgtcatc tggacggttt ccagcgcctg cgtcgcgcgc gcctcgatct ccgccctgcc
     3121 caggcccttt tcctcgagcg ccagcgagac gttgcgctgg accgtcatgt gcggccagag
     3181 cgcatagctc tggaagatca ggccgacccc gcgcttctcc ggcggcacga tcacgccccg
     3241 gcggcgcgag aagaccagct tctcgcccag gtggatgtcg ccgtcgtccg gctcctccag
     3301 ccccgcgatg gcccgcaggg tggtggtctt gccgcagccc gacgggccga ggatcgtcag
     3361 gaactcgccc ggccggatgg tgaggtccag gtcatggacc accacctccc ggccataggc
     3421 cttgcgaatg cccgagaggc cgatctgcgc caccgcgccc ggctactggc tggcgtgcag
     3481 cagccagaag tcgcgcagcc tggggccctc gctctgcatg aagtccgccg aaacggtcca
     3541 gcccttcatc tcgccccatg cctcgccgcc gggcggcagg ggcacgtcgt tgcgcgggct
     3601 gaccgagccc gggtcgtaat acggtgcgag gccctgcagc agctccatgc tccggccctc
     3661 cgtgaagggc ttctccagga ccgaggactc gttcagctcg aggctgccca ggaggtaggc
     3721 ggtgaacacc ttggccgcgt tggggtgggg tgcccggttg gcgatggcgg tgaaggtcgg
     3781 atagacccac ttggcgacgg gatcgaggtc ggccaggtac gcgttgacga agccgtcgga
     3841 ctcgttcttg ctgatatagg tcatgtaggc gaaggcgagg agcggcttct cctgccccgg
     3901 ctggccggag gcctcgacca ggctcgatcc cgacttgaag atcaccacgt cgttctgcag
     3961 cagccgggcg acgaactcgt agcccgcgtc gggcaccccc tcgccgagct cgatgtcatg
     4021 gccggtgtgg cgcttgtagg ccgccgccat ctcgtccgcg tgctggacga tggtggcgag
     4081 ccccatgaag ctcgagcccg acgacatcgg gtcgatgatg cccacacggc cgttgaactc
     4141 gggctcggtc agctcccaga tgttcgtggc cgggggcgcg tcgggatagg cctccttgtt
     4201 gtagtagatg acgatggcct cgttgatgcg gatcagcagc ggctcgcggt tctcttccgg
     4261 aatccggctc ttgtacgcct ccggcacgta gttgaagatc agcccctggt tcagcatctc
     4321 gtggatgacc gccccggaat tgccggtgct cacgacgtcg gcgttgaaga tgccggcctg
     4381 ctgctcgcgg atcgtcttct cgacggtctt gaccgacccc aggtcgaaca ccgtcaagac
     4441 gatgcccgga taggtccggg cgaaggcgtc gaccagcatg gccatgcgcc cggacgagga
     4501 atagacgacg acctcgccct cctcctgcgc cttggcaacg atggcgtccc agccctccgt
     4561 cgccggcgcg tgcgcgccga gctcggccgc cttcagccag gcctccgtct cgggcgacac
     4621 ctcctgggcc gtcgccgcca ccggcagcgc cgccagccac gccagcgcca gagcgcccgc
     4681 ggtcgcgcgc cagtatcgag ctgatctcgt catgtcggct cctgccccct gtcgttgcgg
     4741 cgcggttccg gccgagcccg gcccgccacc cttgtcatcc agcggccgca atcggaccga
     4801 agcgaggcgc caaagtcaac cgggatcgcg ccgccgctcg ccgagccagg catcggcctc
     4861 gggcaggagc gcccgcaacg cgtcgctgcc ctcggcgacg aaccgcgcac cgtgacggcg
     4921 cagcacgtcg gggcggctcg gccgcgggtc cccgggctcg acgaagccga tcgccagcat
     4981 gccggccgcc accgccgcct cgatgccgtg cgggctgtcg tcgatcacca cgcagcgcgc
     5041 cgggtgggcg ccgaattcgg cagcacagag cagcggcagg tccggtgccg gcttgggccg
     5101 ggcgacgagg tcggcgctga agacctggcc agcgaacgcc tgccagaggt cgagccggcc
     5161 gaggctgcgg gtcagccggt agagcgagct gttcgacgcc acgcagcgcg ggcagtccag
     5221 cgcgcgcacg acatcgccga tgccctcgac ctcgcgcaga ttccgaaacc cgccatagag
     5281 ccgcttcgcg tagagcgcga agaactcgtc gagcgccgtc gtgcccagca gctcccggca
     5341 atgcgcccgc gtgtcctctt cggcgccgcc ggtgaagcgc gcatgcgcct cgtccggcgt
     5401 catcacgacc ccgaactcgc ccagcgtctc ggacagggcg aggctggcga tggactcgct
     5461 gtcgatcagc acgccgtcgc aatcgaagat cacgagctcg acatgcgcca gccggatgcg
     5521 cgagaaaccg gctccacggc cggcccgggc gtcagccatg cagccgttcc ttcgccgcga
     5581 cagcgaggtc cgggcggtcc gtggtgatcg agcggatcgg ctgccgcagc cagtggtcga
     5641 tctcggcctc gtcattgacg gtccaggccg cgagacggcc cggctcgacg agcgccgtga
     5701 tggcgtccca atgctcgcgc atcaggtcct tctggatcgc gacgacctcg accagcggct
     5761 cgaaatgccg gaccgccgtc tcgaggccgc cgaggcgcgc cgccgactcg cgatcgatcg
     5821 agcagagccg ccggccctcc ggcagggcgt cgcgaatcag gccgagcacg tcggggtcga
     5881 agctggtgag catcgcccgc tcgccgaagt cgaacccggc cagcaccgcc accaccgcct
     5941 cgacgatgcc gggatagggg acatggtcac catccgactt gagctcgaca tgcaggtcga
     6001 tcctggcatc ccggaagact tccaggacct cggccagggt cggaatggtc tcgccctcgc
     6061 tgccccgcag caccacctca cggtgcgcac acggcgccag ctccgcgacc ttgcccgtcc
     6121 cggttgtcgt ccgctccagg gtcgggtcat gaatgacgag aagctcgccc gccttggtca
     6181 gatgcacgtc gaactcgacc gcgtccacgg ccatctccag gaccttgcgg aagcccagga
     6241 ggctgttctc cggccaaaca ttccggccgc cgcgatggcc gacgatctga accacgatgc
     6301 tcatcctttt tgatatcgct gggttacttg ccggaatcca cgaggccccg cgtgaagagg
     6361 ccctgcagcc cgatgatgat gatcgccggc ggcagcagga cgacgaacgt cgccgccatg
     6421 gcgagattcc aggccggctc cgcgtcggag accggcacga gctgcttcag gccgagcacg
     6481 gcggtcgcca tgtccttgtc cgtggtgaac agcagcggcc agagatactg gttccagcca
     6541 tagatgaaga ggatgatggc gagtgccgcg atattgacct tcgacagcgg cagcaggatg
     6601 tcgaagaaga acttgaccgg cccggcgcca tccagccgcg ccgcctcgca gagctcctcc
     6661 ggcacggtca ggaagaactg ccggaagagg aacgtcgccg aggccgaggc gatcagcggc
     6721 aggatcagcc cccgataggt gttgaccatg ttccactgca gcgccgtctc cagccggtag
     6781 ccggtcaggg tctccaccag cccgctcagc cccgtcacgc cggcgagcca gcgcagcggc
     6841 ccggccgcgt cggcaacggc ctcgtaggtg gggatgatgc ggacctcgac cggcagcatc
     6901 agcgagacga agatcagcca gaatgccgtc atgcggaagg gaaagcggaa ataggtgatg
     6961 gcgtaggcgg cgagcagcga catcaccagc ttgccgatga cgatgccgcc ggtgatgatg
     7021 atcgagttga cgaagagccg gccgaaattc ccccgtgacc acgactcggc caggttctcg
     7081 aaaaaatgcc cgcccggcag caaagggaac ggcgtctgct gcacctcgac gatggtcaac
     7141 gacccggcga cgaaggcgaa gtagagcggc aggcagacca gcaccgcacc aacgaccagg
     7201 atcgcgtggc agacgatgtt gagcgttctg tcctggttca ttcctggttg ctcatacctg
     7261 gtagttcacc ttgcgctcga tggcgcggaa ttggagcacg gtgaacgaca ccgccacgat
     7321 catgagcacg accgactgcg cggccgagga gccgaggtcg agcagcacga aaccgtcctg
     7381 gaagaccttg tagacgagga tgttcgtcgc cccgttcgga ccgccccgcg tcaccgcgtc
     7441 gacgatgccg aaggtctcga acagcgcata gatgaagttc atcaccatca ggaagaagac
     7501 caccggcatg accagcggca gcgacaccgt gaagaaccgg cggatcggcc gggccccgtc
     7561 cagggtggcc gcctcgagca gcgactgggg cacgcccagc agcgacgcca cgagaaagat
     7621 gtagttgtag cagatctgct tccagatcgc ggcgacgatg atcaaaatct gcgcgtccat
     7681 cggccggcgg ttgggatccc attccaggcc gagcgcatgc atgagctggg ccagcggccc
     7741 gaccgccggg ttgaagagga acgcccagag cacgcccgag atcaccgggg cgatggcgta
     7801 gggcagcagg atgacgctct tgtagaggcc gcggccccgg atgacgaagt cggtggcgaa
     7861 ggcgaagacc agggcgatca ggagcgtgcc gatgctgacc accgccgtga agatcagggt
     7921 gaaggcggcg ctgtagcggt actgggcgct gccgaacaac tggcggaaat tgtcgaagcc
     7981 gacgaacagc acgcggccgc cgaacgggtc gatctgcacg aaggcgaggg agagcgcctt
     8041 gtaggacggg atgaagaaga agaagagcag gatgagcagc tgcggcgcca ggaggccggc
     8101 ggcgaaccag ggctgggtga aatgcgcctt cttcagcccg gatgccgacc ggcgcccgtc
     8161 gagcttgagc aggcgcccaa tgccgaaaaa gaaggggcgc ccgggggcgc cccttcctgt
     8221 cgtggccgtt cggtcagccg ccggcattga gccgttcgaa ctggcgcagc acctcgttgc
     8281 cgcgtgcggc cgaattgtcc agcgcctgct gcggtgtcga ctgcccggtc cacaccccct
     8341 ggatctcctc gttggtgatg gcgaagatct ggttgtggtt gccgaaccgg aagccgcgcg
     8401 agttgtccga aggcgtgccg cgattgagct ggacgatggc gatctcgcgg gtcggatgcg
     8461 cctcgaagta gccctcgccc ttcatcatgt cataggcggc gttggtgatt ggcacgtagc
     8521 cggtctgctc gctccaccag gcctgcgtct cgggcgaggc gacgaagttc aggaacgcgg
     8581 cgatcgcctc gtattcctcc tcgctcttgt tctgcagcac ccagagcgcc ccgccgccga
     8641 tcgtgctgtt catcggctcc atgccctcct cgtgcggcat gaacgtggcc ccccagtcga
     8701 actgcgcgcc ggattccacc gcggcgtgca tggccgtcga cgccgtgatc gtcgagcagt
     8761 cgcccgagat gaagagcgcc tcggggttga gcccctggcc ggcgatgtgc agcacgccgt
     8821 cgttcatcca cttctgcaac cgctcgagct gggcgacgat caccgggctc tggttgaagg
     8881 tgaactccgt gtccagcccg ccgaagccgt tggccttcgt gccgaattcc tggtcgtgga
     8941 tggccgagta gttctcgatc aggctccaga cgaagtcgtt gggcagcgac atcgggcact
     9001 cgctgatgcc ctgctccttg atcgcgtaga gctgcttctc gacctcctgc caggtctcgg
     9061 ccgggccgtc gggaaaacct gcggcctcga agttgtcggc gttgtaccag aagatcggcg
     9121 tcgagctgtt gaacggcatc gccgccggct tgccgtcgac caggtagaag ccggcgaccg
     9181 gggcgatgaa gttgtcccag tcgatatcgt agccttccct ggccatcagc tccggcaccg
     9241 gcacgatcgc acccgagttg tacatggtca ggaacgcgcg ctcggacgtc tgcaccaggg
     9301 tcggatgctc gttcacgcga taggccgcaa ccatggcggc ggcagtctcc tcgtaattgc
     9361 ccttgagcac gccgacgatc tcgtacttgt cctgcccggc attgaagttc gcgatcatct
     9421 cctcgacgat cggcgtgagc cggccacgca tcgcatgcca ccactcggcc ttgatcgtct
     9481 ccgccgccag ctcgccgccc gccagcggca gcatggccac cgtcaaagcg agcacgccgg
     9541 cgactttccg cgccgtcgac cggcccggct tcctccaccg cttcagtagg gacatgagtg
     9601 gcctcctctc tctattattg gttgggctgc gttgcatcgg cgctgttcgg caggcgcgcg
     9661 gggcccgacc gagccggccc cgtcgcggta acgccacttg cagtaacgcc tacctaggag
     9721 aagtgtcaat cggctgttac agaggaaaaa accgcgcccg ggaaggcagt gcatggccga
     9781 tatccacctg aacgacctga gaaaaagcta cggcaccgtc gaggcggtga agggcgtctc
     9841 gctggccgtg caggacggcg agatgatcgt cctggtcggg ccgtccggct gcggcaagtc
     9901 caccctgctg cgcatgatcg ccggcctcga atccatcacc gcgcgcgagc tccatatcgg
     9961 cggccgggac gtcagccgcg tcgagccggc cgagcgcgac atcgccatgg tcttccagaa
    10021 ctacgccctc tacccgcaca tgtcggtccg ccagaacctc tcctacgggc tgaggaaccg
    10081 cggcgtcccc cgccaggaga tcgcccttcg cgtcgacgac gtggcacgca tcctcgagct
    10141 cggcatgctc ctcgaccgcc gccccggcca gctctccggc ggccagcggc agcgcgtcgc
    10201 catgggccgc gccatcgtcc gcaaccccgc cgccttcctc ttcgacgagc ccctctccaa
    10261 tctcgatgcc aggctccgcg tgcagatgcg cgtcgagatc cgcaagctcc agcgccggct
    10321 gcgcaccacc agcctctacg tcacccacga ccagctcgag gccatgaccc tggccgaccg
    10381 cctggtcgtc atgaacgccg gcgagatcga gcagatcggc acgcccctcg aggtctatgt
    10441 ccgccccgaa accctcttcg tcgccggctt catcggctcg ccgcccatga acctgctgcc
    10501 gctggacatc ctggc
//
LOCUS       DAOTFI010000176        10370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7634_length_10370_cov_4.744353, whole genome shotgun sequence.
ACCESSION   DAOTFI010000176 DAOTFI010000000
VERSION     DAOTFI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7634_length_10370_cov_4.744353"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..347
                     /locus_tag="P5558_16630"
     CDS             3..347
                     /locus_tag="P5558_16630"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRY25997.1"
                     /translation="MAMPADTNPSGDIFGGWLLAQMDVAGGIVAYERAAGRVATVAVD
                     GMTFHHPVYVGDVVSCYAEVASPGRTSLTLQVEVWVQRARTGEPMKVTEGRFTYVAIG
                     ADGKKRPLPERP"
     gene            344..1474
                     /gene="tgt"
                     /locus_tag="P5558_16635"
     CDS             344..1474
                     /gene="tgt"
                     /locus_tag="P5558_16635"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRY25998.1"
                     /translation="MSVGFELLAEDGAARRGRLRTAHGMVETPAFMPVGTAATVKAMT
                     VEMVEATGAEIILGNTYHLMLRPGAERIAGLGGLHRFMRWPGPILTDSGGFQVMSLAR
                     IRKLDERGVVFASHHDGSRHELTPERSIEIQDLLGADITMQLDECLALPAPAGEVERA
                     MRLSLRWGERSKAAFRPREGHGLFGIVQGGTDAGLRRASAEGLVDIGFDGYALGGLAV
                     GEPQAVMFETIEATVPHLPEDRPRYLMGVGKPLDILGAVLRGVDMFDCVLPTRSGRTG
                     QAFTRFGSVNLRNARHADDPRPLDESSDCPAARDYSRAYLHHLVRSGEILGAILLTWN
                     NLHFYQAMMRGVRAAIEAGEAMAFAERFAEGQARGDIPPHAG"
     gene            1522..1597
                     /locus_tag="P5558_16640"
     tRNA            1522..1597
                     /locus_tag="P5558_16640"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1555..1557,aa:Lys,seq:ttt)
     gene            complement(1768..2856)
                     /locus_tag="P5558_16645"
     CDS             complement(1768..2856)
                     /locus_tag="P5558_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY25999.1"
                     /translation="MPAPRHFHERPNRDGSIRLYWRPSASLRKLGFRDRRLSDDRSRA
                     LAECEAHNREVDRYRLTMAQGVFDRARPGTLRHLIAAYKAHDDYLLLAPSTRRDYGHR
                     LEIIALRIGDARTRELKPRVVQALKRSFAATPSQANHVLRVFRLLCSFAVREGIMADN
                     PALAFRQYRERPRRQLWRHDQERRFLEVCGPDLALVYLLAVYTAQRQGDLLALPWSAY
                     DGEVIRLEQGKTGALLEIPVAARLARVLAETPRAGPMICTREPLRVPGGRPAGPIRLP
                     WTADHFRHRFAAARKAAGIEDRRFQDLRRTAIVRLAEAGCTVPEIASISGHAIDYCAR
                     IIETYLPRTRSLARSAITKLEDHRPREP"
     gene            complement(2810..3079)
                     /locus_tag="P5558_16650"
     CDS             complement(2810..3079)
                     /locus_tag="P5558_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26000.1"
                     /translation="MQRYLNEIELAYLLGFRRWRDFEKALRGGHVPAPDRQYPDGPRW
                     SGVLVESQLSGRLDTLEAMGREDALIERLESAGTAPLPRAAKSRR"
     gene            complement(3108..3365)
                     /locus_tag="P5558_16655"
     CDS             complement(3108..3365)
                     /locus_tag="P5558_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26001.1"
                     /translation="MSDERDHCPRCRAVLNANLSELTDDGPADAGTIGVCGVCGELLM
                     FCDPGLRALTEAERVALMAHPRAGPAVLDFLERLIRQKRRP"
     gene            complement(3362..3637)
                     /locus_tag="P5558_16660"
     CDS             complement(3362..3637)
                     /locus_tag="P5558_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26002.1"
                     /translation="MRGEPPYFRIVGAPDTLIVRPLDRSVLIEIGRPGPSGEFQLAGA
                     ACLTPEEVQGVIDAMRHARRLVRQRQGYHRRAAASMDPTNNHPGRHR"
     gene            complement(3634..3924)
                     /locus_tag="P5558_16665"
     CDS             complement(3634..3924)
                     /locus_tag="P5558_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26003.1"
                     /translation="MSAGPLYAWLIRAPDLSTPLLNFTDKSARLVFVRFEDALLARDS
                     ALALAEQGGYPVDLVMFRAGQPFLTIDPSEWRDDDPHVRLGQPPAPAEEPRS"
     gene            complement(3921..4613)
                     /locus_tag="P5558_16670"
     CDS             complement(3921..4613)
                     /locus_tag="P5558_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26004.1"
                     /translation="MTPLALAALEREKERQRQAQRRARERREAAEERARQAEAAAKER
                     RRLAAIERNRQAAAAAERRAAERRAAAPFAETVALNIYARRTAAGIRYAVIIDGQCMT
                     FDDLEEARDWRNRFAGSRRESRPYLFVNLIRAGWYGRVSGFREELRSFILARTQSPGL
                     AAATLVEVLAIIVQTTACQRDTEEETRDSAERLAADLCRTLMMTATDPEKLEEGRRVA
                     AELRAMLRRRPS"
     gene            complement(4610..4942)
                     /locus_tag="P5558_16675"
     CDS             complement(4610..4942)
                     /locus_tag="P5558_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26005.1"
                     /translation="MIALAMVDSLEPPADPAELDAVLRRAMRLAELVMATTDEATADD
                     PFGRMVLGLALQRCLAGFYARMALDHEASELAPEIAGRAVRIAMEATAELAERLVAAG
                     QHMPAPPP"
     gene            complement(4939..5157)
                     /locus_tag="P5558_16680"
     CDS             complement(4939..5157)
                     /locus_tag="P5558_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26006.1"
                     /translation="MNDAPSRPTTEAILADLFDALEEAALDLARLHGTDPVRMERLMT
                     ELGTRAAVRVRVAIRAERRAALEQRRLR"
     gene            complement(5154..6032)
                     /locus_tag="P5558_16685"
     CDS             complement(5154..6032)
                     /locus_tag="P5558_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26007.1"
                     /translation="MSATVAAPGLYRMAAAAYHADPAPAPSLSSSLARLIVDQSPAHA
                     KEAHPRLTLDPIGPESKAIDRGAAMHELLLGGESRIQTIDAADWRTRAAQAARAEARA
                     AGRIPVLAADWPAMARMLAELRRFLAQARLADVFEAGAGEAELSGFWQEGGIWRRARF
                     DWLDRRTATVWDYKTTALPLGRWRANAARDPVAAIQPIHYRKAALALTGRRHRFAYVV
                     QEVRPPFAVILIEPGDRLLKRALTGYENAAAAWRAGLERGHWPAYPARLDRLDPPTPP
                     RSAKGRRPWPFRKRAP"
     gene            complement(6029..6247)
                     /locus_tag="P5558_16690"
     CDS             complement(6029..6247)
                     /locus_tag="P5558_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26008.1"
                     /translation="MTDTPASIAAAELAALLASGEHRVVGGAIVTEAEYLAMIDAELD
                     AYFGTEGDASPTEIDTDEHSDTLPEPGR"
     gene            complement(6333..6671)
                     /locus_tag="P5558_16695"
     CDS             complement(6333..6671)
                     /locus_tag="P5558_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY26009.1"
                     /translation="MEEPHERLRQARIGAGFKTAADFARSFGIEPGTYNHHETGVRGF
                     ARFAHRYARALNVSVEWLLGQPDASPNDPAEMAAELARRMTPEQRAAWFSVGSALAQS
                     TDRDQAGNDS"
     gene            6755..7012
                     /locus_tag="P5558_16700"
     CDS             6755..7012
                     /locus_tag="P5558_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26010.1"
                     /translation="MNSFREVIRAWPDTATLAGDIGARTKTVRQWLFRDRIPAPFWSP
                     VSDAARKRRIPGVTVERLASLGATGKAPAAIVAERQPEHVA"
     gene            complement(7039..7458)
                     /locus_tag="P5558_16705"
     CDS             complement(7039..7458)
                     /locus_tag="P5558_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26011.1"
                     /translation="MQEPPEWYDRKKGFRPEFDRGALYNREWPKNGHDEATARLMNRL
                     MLWTAIICTLLGIAFGVRDALASCEQLLGTGYRCKTPMGESVTVTPTLGGAHRYDYQN
                     GGFAIERPNFNGGYSYSTTGRTGDLPMEGGTVFRPYD"
     gene            8005..8757
                     /locus_tag="P5558_16710"
     CDS             8005..8757
                     /locus_tag="P5558_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26012.1"
                     /translation="MSWAASFDPHDLQPIVKLPDLIEQALDQQTPATRRAALDLIRTA
                     WRTRGMTLDASVATVRSITRKGVKMALRFIARLAELFGLEGGRFTFPFWDEERKKREL
                     RLARCSRGGTTTSLRRQARRLTQEERRISDARLRSYRKRVARTAERIELAIGKTQILR
                     FELHGTPIDEDYEPGTPDEQPGRLAGRLARAAELVLGLSEAEARVRGQSLAKLHRMHA
                     RRILSISDSIVAMLCGGDFFLNDWLRAHPPDG"
     gene            9231..9650
                     /locus_tag="P5558_16715"
     CDS             9231..9650
                     /locus_tag="P5558_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26013.1"
                     /translation="MNDQARAARAEKSRALRAELFAVCDDSEHDHDGKINALCWTIAE
                     LAAQEAFMRGENELELSIRMMGARQWIDSKSRDWLTHLREQPYEEMRARIVERNEAAR
                     ARWRERRELARAAAAGAATAGESDTGRVILPGEPSGG"
     gene            complement(9966..>10370)
                     /locus_tag="P5558_16720"
     CDS             complement(9966..>10370)
                     /locus_tag="P5558_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26014.1"
                     /translation="AADAAFQAQGEADLAELRQAWGAAFDARAEAGRRAARQFGFSAE
                     ELGKIERAIGTKGLLERMATIGAALGEDGGVPSAAAAHAGAGSPAAARAEIETLKADR
                     EFLAAYLDPRSPAHQGAKAKMNRLMEAAARAG"
BASE COUNT         1471 a         3464 c         3888 g         1547 t
ORIGIN      
        1 ccctggcgat gccggcggac accaatccct cgggcgacat cttcggcggc tggctgctcg
       61 cccagatgga cgtcgccggc ggcatcgtcg cctatgagcg ggcggcgggg cgggtcgcca
      121 ccgtggcggt cgacggcatg accttccatc acccggtcta tgtgggcgac gtggtgagct
      181 gctacgccga ggtggccagc cccggccgca cctcgctgac gctgcaggtc gaggtctggg
      241 tgcagcgggc caggaccggc gagccgatga aggtgaccga aggccgcttc acctacgtgg
      301 cgatcggcgc cgatggcaag aagcggccct tgccggagcg gccttgagcg tcggcttcga
      361 gctgctggcg gaggatggcg ccgcccggcg gggccggctg cggacggcgc acgggatggt
      421 cgagacgccc gccttcatgc cggtcggcac ggccgccacg gtgaaggcga tgaccgtcga
      481 gatggtcgag gcgacggggg cggagatcat cctcggcaac acctatcatt tgatgctgcg
      541 ccccggggcc gagcggatcg ccggactggg cgggctgcac cgcttcatgc gctggcccgg
      601 cccgatcctc accgacagcg gcggctttca ggtcatgtcg ctcgccagga tccgcaagct
      661 ggacgagcgg ggcgtggtct tcgcctcgca tcacgacggc agccggcacg agctgacgcc
      721 cgagcgcagc atcgagatcc aggacctgct cggtgccgac atcaccatgc agctcgacga
      781 gtgcctggcg ctgccggcgc cggcggggga ggtggagcgg gcgatgcggc tgtcgctgcg
      841 ctggggcgag cgctcgaagg ccgccttccg gccgagggag ggccacggtc tcttcggcat
      901 cgtccagggc ggcaccgacg ccgggctcag gcgggcctcg gccgagggcc tggtcgacat
      961 cggcttcgac ggctacgcgc tcggcggcct cgccgtcggc gagccgcagg cggtgatgtt
     1021 cgagaccatc gaggcgaccg tgccgcatct gccggaagac cggccgcgct acctgatggg
     1081 tgtgggcaag ccgctcgaca tcctgggtgc cgtgctgcgc ggcgtggaca tgttcgactg
     1141 cgtgctgccg acgcgctctg ggcggaccgg gcaggcgttc acccgtttcg ggtcggtgaa
     1201 cctcaggaat gcccgccatg ccgacgatcc gcgaccgctc gacgagtcgt ccgactgccc
     1261 ggcggcgcgc gactattcca gggcctatct gcaccacctc gtgcgctcgg gcgagatcct
     1321 gggggcgatc ctgctcacct ggaacaacct gcatttctac caggcgatga tgcggggcgt
     1381 gcgcgccgcg atcgaggcgg gcgaggccat ggccttcgcc gagcgcttcg ccgaggggca
     1441 ggcgaggggc gacatcccgc cccacgccgg ctgagccgcc gggttgacgc cgccccgccg
     1501 ttgtcggata gcacggcccg ggcgcctcta gctcaattgg cagagcagcg gacttttaat
     1561 ccgcgggttc tgggttcgag tcccaggggg cgcaccattt caatgactta gcccggttga
     1621 atttggggcg cgtgtgactc tcgtggggtc gcgtgcaact gttctggtcg gattcgggtc
     1681 gaactggggc gagcgccggg gccggcccgg cgggccgata acctgcggtg gaatgctcgg
     1741 cgtttcagcc tgtgttgcgt cgcaaattca cggctcgcgg ggccggtggt cctcgagctt
     1801 ggtgatcgcc gaccgggcca ggctgcgcgt ccgggggaga taggtctcga tgatgcgggc
     1861 gcagtagtcg atcgcatggc cggatatgct ggcgatctcc ggcaccgtgc agccggcttc
     1921 ggcgagccgc acgatcgccg tccggcgcaa gtcctggaag cgccggtcct cgatgccggc
     1981 ggccttgcga gcggcggcga accggtggcg gaaatggtcg gccgtccagg gcaggcggat
     2041 cgggccggcc ggccggccgc cgggcacgcg caaaggctcc ctcgtgcaga tcatcgggcc
     2101 ggcgcgcggc gtctcggcca gcacgcgggc gagccgggcc gccaccggga tctccagcaa
     2161 ggcacccgtc ttgccctgct cgaggcggat cacctcgccg tcataggcgg accagggcaa
     2221 ggcaaggagg tcgccctggc gctgtgccgt gtagacggcg agcagataga ccagcgccag
     2281 gtcgggcccg cagacctcga gaaagcgccg ctcctggtcg tggcgccaga gctggcgccg
     2341 aggccgctcc cggtactggc ggaaggcgag cgccgggttg tcggccatga tgccctcgcg
     2401 cacggcgaaa gagcagagca gccggaaaac ccgtagcaca tggttcgcct ggctcggcgt
     2461 cgcggcaaag gatcgtttca acgcctggac gacgcggggc ttgagctcgc gggtgcgcgc
     2521 gtcgccgatc cgaagcgcga tgatctcgag ccggtggccg tagtcgcgcc gcgtgctcgg
     2581 cgccagcagc agatagtcgt cgtgcgcctt gtaggcggcg atcaggtggc gaagcgtgcc
     2641 gggccgggcg cggtcgaaca cgccttgcgc catggtgagc cggtagcgat cgacctcgcg
     2701 gttgtgcgcc tcgcactcgg ccagggctcg cgagcggtcg tcggaaagcc gccggtccct
     2761 gaaaccgagc ttgcggagcg atgccgacgg gcgccagtag agccggatgc taccgtcgcg
     2821 atttggccgc tcgtggaagt ggcgcggtgc cggcactctc gagcctctcg atcaaagcat
     2881 cttcccggcc catagcttcc aacgtgtcga gccgaccgga caactgcgat tcgaccagca
     2941 cgcccgacca gcgcgggccg tcgggatatt gccggtccgg cgccggcaca tgcccgccgc
     3001 gcagcgcctt ctcgaagtcg cgccagcggc ggaagccgag cagataggcg agctcgatct
     3061 cgttcagata gcgttgcacg gcgtttcatt ccggggggcg gggcgtttca tgggcggcgc
     3121 ttctggcgga tcaggcgctc gaggaaatcg agcacggcag gaccggcccg gggatgcgcc
     3181 atcaaggcga cgcgctcggc ctcggtgagg gcgcgcagac ccggatcgca gaacatcagg
     3241 agctcgccac agacgccaca gacgccgatg gtgccggcat ccgccgggcc gtcgtcggtg
     3301 agctcgctca aattggcgtt cagcacggcg cggcacctgg ggcagtggtc gcgctcgtcg
     3361 ctcaccggtg gcgccccggg tgattgttgg tcgggtccat gctggcggcg gcgcggcggt
     3421 ggtagccctg gcgctgccgg accagccggc gggcgtggcg catggcgtcg atcacgccct
     3481 gcacctcctc cggggtgagg caggcggcgc cggcgagctg aaactcgccg ctcgggccgg
     3541 gccggccgat ctcgatcagc accgatcggt cgagcggccg gacgatcagc gtgtcgggcg
     3601 cgccgacgat ccggaaatac ggcggctcgc ctctcacgat cgcggctcct cggcgggggc
     3661 gggcggctgc ccgagccgca catgcgggtc gtcgtcccgc cattcgctcg ggtcgatggt
     3721 caagaacggc tggccggccc tgaacataac gaggtcgacc ggatagccgc cctgctcggc
     3781 cagggccaag gcgctgtcgc gcgccagaag ggcgtcctcg aaccgcacga aaaccagccg
     3841 ggccgacttg tcggtgaaat tgagcaaggg ggtgcttagg tcgggggcgc ggatcagcca
     3901 ggcatagagc gggccggcgc tcacgacggc cgccggcgca gcatggcgcg aagctcggcg
     3961 gcgacgcggc ggccttcctc gagcttctcc gggtcggtgg cggtcatcat cagcgtcctg
     4021 caaagatcgg cggcgaggcg ctcggcgctg tcccgggtct cctcctccgt gtcccgctgg
     4081 caggcggtgg tctgcacgat gatggcgagc acctcgacca gcgttgcggc ggcgaggccg
     4141 ggcgactgcg ttcgggcgag gatgaaagag cgaagctcct cgcgaaagcc gctgacgcgc
     4201 ccataccagc cggcgcggat caggttcacg aagagatagg ggcggctctc gcggcggctg
     4261 ccggcgaacc ggttgcgcca gtcgcgggcc tcctcgaggt cgtcgaaggt catgcactgg
     4321 ccgtcgatga tgacggcgta gcggatgccg gcggcggtcc gccgggcgta gatgttgagg
     4381 gcgaccgtct cggcgaaggg tgcggcggcg cggcgctcgg cggcgcggcg ctccgcggcg
     4441 gcggcggcct gccggttgcg ctcgatcgcg gccagccggc ggcgctcctt cgcggcggcc
     4501 tcggcctgcc tcgcccgctc ctcggcggcc tcgcggcgct ccctggcgcg gcgctgtgcc
     4561 tgtcgctgcc gttccttctc ccgctcgagg gcggcgaggg cgaggggggt catggcggcg
     4621 gcgccggcat gtgctggccg gcggcgacca gccgctcggc gagctcggcc gtggcctcca
     4681 tcgcgatccg gaccgctcgc ccggcgatct ccggggcgag ctcgctcgcc tcgtggtcga
     4741 gcgccatgcg cgcatagaag ccggcgaggc acctttgcag ggccaggccg agcaccatcc
     4801 ggccgaaagg gtcgtcggcc gtcgcctcgt cggtggtcgc catgaccagc tcggcgaggc
     4861 gcatggcgcg gcgcagcacg gcgtcgagct cggccggatc ggccggcggc tcgaggctgt
     4921 ccaccatggc gagggcgatc atcgaaggcg ccgctgctcg agggcggcgc ggcgctcggc
     4981 ccggatggcg acgcgcaccc gcacggcggc gcgggtgccg agctcggtca tcaggcgctc
     5041 catccgcacc gggtccgtcc cgtgcagccg ggcgaggtcg agggcggcct cctcgagggc
     5101 gtcgaagagg tcggccagga tggcctccgt cgtcgggcgg ctcggtgcat cgttcacggg
     5161 gcgcgcttcc tgaacggcca cgggcggcgg cccttggcgg atcggggggg cgtcggcggg
     5221 tcgagccggt cgagccgggc gggataggcc ggccagtggc cacgctcgag cccggcgcgc
     5281 catgcggcgg cggcgttctc gtagccggtg agcgcgcgtt tgaggagccg gtcgccgggc
     5341 tcgatcagga tcacggcgaa gggcgggcga acctcctgca ccacataggc gaagcgatgc
     5401 cgccggccgg tgagggcgag ggcggccttg cggtagtgga tgggctggat ggcggccacc
     5461 gggtcgcggg cggcgttggc gcgccagcgg ccgagcggca gggccgtcgt cttgtagtcc
     5521 cataccgtcg cggtccttcg gtcgagccag tcgaaccggg cgcggcgcca gatcccgccc
     5581 tcctgccaga agcccgaaag ctcggcctcg ccggcgccgg cctcgaacac gtcggcaagc
     5641 cgggcctgcg ccagaaagcg gcgaagctcg gccagcatcc gcgccatcgc cggccagtcg
     5701 gccgccagca cggggatgcg gccggccgcg cgggcctcgg cgcgggcggc ctgcgctgcc
     5761 ctggtgcgcc agtcggcggc gtcgatcgtc tggatccggc tctcgccacc caagagcaat
     5821 tcgtgcatgg cggcgccgcg gtcgatcgcc ttcgattccg ggccgatcgg gtcgagggtg
     5881 agccgggggt gcgcttcctt cgcatgggcc gggctctggt cgacgatcag ccgggcgagg
     5941 ctcgaggaca gagacggcgc cggggcgggg tcggcatggt aggcggcggc ggccatgcgg
     6001 tagaggccgg gggcggcgac ggtggcgctc accggccggg ctccggcagc gtatcggagt
     6061 gctcgtccgt gtcgatttct gtcgggctgg cgtcgccctc ggtgccgaaa taggcgtcga
     6121 gctcggcgtc gatcatcgcc agatactcgg cctcggtgac gatcgcgccg ccgacgaccc
     6181 ggtgctcgcc cgaggcgagc agagcggcga gctcggcggc ggcgatgctg gcaggcgtgt
     6241 cggtcatggc ggcggctccc gtgttgtggg aacacgctac gttttgtaga gtggctttgc
     6301 aagggataac ctacgtaccg tagggactgg ccctagctgt cgttgccggc ctgatccctg
     6361 tcggtcgatt gagcaagcgc agacccaacc gaaaaccacg ccgcgcgctg ctccggggtc
     6421 attcggcggg cgagctcggc ggccatttcg gccggatcgt tgggcgatgc gtcgggctgg
     6481 cccaaaagcc actcgacgct aacattgagc gcgcgagcgt agcggtgggc gaaccgggcg
     6541 aagccgcgca cgccggtctc gtggtggttg taggtgccgg gctcgatgcc gaaggaacgc
     6601 gcgaaatcgg cggccgtctt gaatccggcg ccgatcctgg cctgcctcaa tcgttcgtgg
     6661 ggttcctcca tgactacaga ttgtagatta gggcgcgaga atcgcacgct acgtttcgac
     6721 ttgcattcgt ccctacgaaa cgtagtgtat ggggatgaac agctttcgcg aggtgatccg
     6781 cgcgtggccg gatacggcga cgctggcggg cgacataggg gctcgaacga aaaccgttcg
     6841 gcagtggttg tttcgtgatc gcatcccggc gccattctgg tcccctgtgt cagatgccgc
     6901 ccgcaagcga cggattccgg gtgtgaccgt ggaacggctg gccagcctcg gggcgaccgg
     6961 aaaggcgccg gcggcgatcg tcgccgagcg tcagccggag catgtcgcgt gagaggctca
     7021 cccgggccgg aggtcgcctc agtcgtaggg ccggaacaca gtgccgccct ccatcggcag
     7081 atcgccggtt cgtcccgtcg tcgagtagga atagccgccg ttgaagttgg ggcgctcgat
     7141 ggcgaagccg ccgttctggt agtcgtagcg gtgcgcgccg ccgagggttg gcgtgacggt
     7201 caccgattca cccatcggcg tcttgcagcg atagccggtt ccgaggagtt gctcgcaact
     7261 ggcgagcgcg tcgcgcacgc cgaacgcgat gcccagaagc gtgcagatga tagcggtcca
     7321 gagcatcagg cgattcatca gccgggcggt ggcttcgtca tgcccattct tcggccactc
     7381 cctgttgtag agggcgccgc ggtcgaactc cggccgaaag cccttcttgc ggtcgtacca
     7441 ttccggcggc tcctgcatcg ctctgccctc gcatggtggt ccgggcagga aaccgcatcg
     7501 cggggcggcg cggggcgccg atcggcgccg ggcatagccg gcggggccgg gccgttaacc
     7561 atgacggggc ggcggccgtg acggtgctcg agggcttcgc cctggcgctc gccgtctacg
     7621 cggcgatcat ggcggcgatc tgggtgccgc ggctctgggc cgtgctccac gccttccggc
     7681 atcggccctg agccggtggc gacaatcaga aaagcgggcg ccaggcgcct gggggaaacg
     7741 ccggcgcgcg ccggcttcgg atcacgctcg gaccaaagaa gaagcgacgc cccggggagg
     7801 ggcgccgctt cgtgtagtcc gatcgttcaa gtccacggcc gcgaccacgg ccggctgcgt
     7861 ctcgtgtgct gtcccaccgc caaagtgcaa ccgaaacaac gacgacgaca tgcgtaggat
     7921 cggccgtggc tggccgattc tcaagccctt cattgcgata atcgcctgcg accgcgcccc
     7981 accgctgggt gaacggaggc gggcgtgagc tgggctgcct cgttcgaccc gcatgacctg
     8041 caaccgatcg tcaagctgcc ggacctgatc gagcaggcgc tcgatcagca gacaccggcg
     8101 acacggcggg cggctctcga cctgatccgc accgcctggc gcacacgcgg catgacgctc
     8161 gacgcgagcg tcgccaccgt tcggtcgatc acgcgcaagg gcgtcaagat ggccctgcgc
     8221 ttcatcgccc gcctggccga gctgttcggc ctcgagggcg gccgcttcac ctttccgttc
     8281 tgggacgagg agcgcaagaa gcgggagctg cgcctcgccc gctgctcgcg cggcgggacg
     8341 acgacctcgc tccgccggca agctcggcgg ctcacgcagg aggaaaggcg gatcagcgac
     8401 gccaggctgc gcagctaccg caagcgcgtc gcccggacgg cggagcgcat cgagctggcg
     8461 atcggcaaga cccagatcct gcgcttcgag ctgcacggca cgccgatcga cgaggattac
     8521 gagccgggca cgccggacga gcagccgggc aggctcgccg ggcgcctggc gagggcggcc
     8581 gagctggttc tcggcctgag cgaggccgag gcgcgggtgc gaggccaatc gctcgccaag
     8641 ctgcatcgga tgcatgcgcg gcggatattg agcatcagcg attcgatcgt ggcgatgctc
     8701 tgcggtggcg acttctttct caacgactgg ctgcgggcgc atccgcccga cggctagcgc
     8761 cgcgccgttt tccgacatcg ttgacccgat cgccggctcc cggccacccc ggcgcacgcc
     8821 cggcctctca atcccgaagc tgaaaagggc gcctcggcat cgctttcccg gcgtggtccg
     8881 atgctgccgc cgctcgcgcg ctccccggcg ccgccccggg gcctgcccgg ctgtggaaaa
     8941 atgcggggcg acttctcgcg ctggttgaca aaattccgaa atgctgcgga agcgaacaat
     9001 caagcatctg taattccctg ataatgcatg cgctgcactg gcacctctcg gagtcctatt
     9061 cttaaagaat cagactcctt cctgtagggg cgagcccggc tgtggataga cgagcgcgaa
     9121 ccgtcgccaa aggcgaccgt tccgccaagt cattgacgtt tttgaagcca tcccggtcgc
     9181 gccggtgtcg gctaggctgc tcgcaggagg ggcggcggtg aggactcgcg atgaacgacc
     9241 aggcacgagc ggcgcgcgcc gagaagagca gggcgctgcg cgcggagctg ttcgccgtct
     9301 gcgatgactc ggagcacgac cacgacggca agatcaacgc gctctgctgg accatcgccg
     9361 aactcgcggc acaggaagcg ttcatgcggg gcgagaacga gctcgagctg tccatccgca
     9421 tgatgggcgc gcgacagtgg atcgacagca agagccgcga ttggctgacg catctgcggg
     9481 agcagccata cgaggagatg cgggcgcgga tcgtcgagag gaacgaggcg gcgcgcgcac
     9541 gctggcgcga acgccgcgag ctcgctcggg cggccgccgc cggggccgcg acagccggtg
     9601 aaagcgatac gggacgggtg atcttgcccg gcgagccgag cggcggctga cggggctccc
     9661 gggcgccctg gcggcgggtt cgcctgccgg tgtcatcggc gccgggcgcg tgcgatcggg
     9721 ggtttcgcgc cgccggcccg gcacggcgct tgaccgtcgc ggccggagcg gctggaaaga
     9781 acgggcggcc gggcgcttca gttagcgccg atgcgtccgg ccgctctggt ctttccgttg
     9841 tttcctcacc ggcaagcgaa gggggcggcg ccggggcaag cggcgccgct ccaccttcga
     9901 tcagaagagt tgccggcgga tgcgaaagtt tcgccgcgtc tcggccagta ccgtcaattc
     9961 gccgatcagc cggcccgggc ggcggcttcc atcaggcggt tcatcttcgc cttggcgccc
    10021 tggtgcgccg ggctcctcgg gtcgagatag gccgcgagaa actcgcgatc ggccttcagg
    10081 gtctcgatct cggcccgggc ggcggccggc gagccggcgc cggcatgggc ggccgcagca
    10141 ctcgggacgc cgccatcctc gccgagcgcg gcgccgatgg tggccatgcg ctcgagcaag
    10201 cctttcgtgc cgatcgcccg ctcgatcttg ccgagctcct ccgcgctgaa cccgaactga
    10261 cgggccgccc gccggccggc ctcggccctg gcgtcgaacg ccgcacccca tgcctggcgg
    10321 agctcggcaa ggtcggcctc gccctgcgcc tggaaggcgg cgtcggcggc
//
LOCUS       DAOTFI010000177        10341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7660_length_10341_cov_7.818783, whole genome shotgun sequence.
ACCESSION   DAOTFI010000177 DAOTFI010000000
VERSION     DAOTFI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7660_length_10341_cov_7.818783"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..697
                     /locus_tag="P5558_16725"
     CDS             284..697
                     /locus_tag="P5558_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26015.1"
                     /translation="MARARLTLACCVLAMGLAPPVAGSAIEKAPRSATGPVQVDPDSG
                     VQIRVGKVTSVRTEGGRTRFRIDSCPFQPTNPTFFAIASDNPDKLDITRLLISAMIFD
                     REVKVYNTSFGEDVDWCPGTRVIGDITVGTVRASD"
     gene            723..1010
                     /locus_tag="P5558_16730"
     CDS             723..1010
                     /locus_tag="P5558_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26016.1"
                     /translation="MLAACALAAFLLPPMHADARRGIVSEVTSVGGQLRFWLAECGSN
                     AFFYFTADDPSRDQAKLIVLAAKFSGQPVAVTAGCPAQGSTRVTDLELVGY"
     gene            1035..3014
                     /locus_tag="P5558_16735"
     CDS             1035..3014
                     /locus_tag="P5558_16735"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRY26017.1"
                     /translation="MDAFRLRHLLSSTLLAAALVATSPAAFAVKPYEEGEGGSTSQPD
                     LDDVLEEHGNDTESSGNGPTGNTGTPRAVTVDGFALADVPRTGPRLATGYAMTHEHPT
                     HGMAFGGNYGFAGHEGNYVNGIPEDGYQNQGACSGCCDHGEFKGNLIPYFQFSDMGRH
                     DPHGGARFDSFSHLRYSSDWIENALDPGPGVGADTSMKILVAFAVESESMCELVYYDN
                     RGKGGAGGAGYPCSEGDSLASLVRQIDALKAWAAAHDWAEIAYSAAEARAIANAGKLA
                     VVLGVEADYTWGAEDRTFDPVARLRDYRDMGVRTFYLAHKINSRLAGADIFRSPSEQD
                     GKSIRALQGIAGCFYYDDNVGEFPLGSYCDNFSRCGANAFRGPLPTDQCATKVSDISE
                     ANLALYLLTGSINGFAVYPRPPGFSDPSGGSRMDGAVERNNLSLSIEGSRVVVEAMRH
                     GMIMDLGHVSSKARTDIHDHSQAFYNYPLNALHNNPNEMLIEPAPNEYDFDKSERDYV
                     RETGGMFGLRLGPVDARSYAASGVTANCPKTSTENAKILAYLLDEGQRVGYSLDFATV
                     TEGTHSRTLENCGIALGHDYIDRYAATGAPNDTVTHGLAHIGNMEAWHDELLTVGLKQ
                     RYVDKLENQSAEWFLRMWEKSEFTARMPPTVMAIL"
     gene            3034..3801
                     /locus_tag="P5558_16740"
     CDS             3034..3801
                     /locus_tag="P5558_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26018.1"
                     /translation="MPTRPKSPVPCAAAASAPRLPPAMAVACAIVLLAMPAFAAKPYQ
                     EGEGGSSSQPGPGDFGVELPGGGQTVEPLFVTGRVTSLRLQKLGNPLVYFSLDDPRGC
                     TAHPEVYFFDGNDPDYAYALKALVASRLSGREVKLEGMKGCPGPPVGIDASAVHIIES
                     SPEAGPVAAPADGLFGEVTNLMIKEGSVYFRLSACSGSAPYFTFAADLPGGFALLLAA
                     QSATRPVRVETTIGSTSTCPTAGGGDVELQGGSLWLL"
     gene            3789..4118
                     /locus_tag="P5558_16745"
     CDS             3789..4118
                     /locus_tag="P5558_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26019.1"
                     /translation="MAALGGSSGTALAAAAIAACLGPSIAHALDGGVKELWTESGRVR
                     FMLDTCATDVDFELAATDADASMAYDILLSTLLARGRVRIYDRQGGPIASCPQRDTQI
                     GSVRALP"
     gene            4217..5077
                     /locus_tag="P5558_16750"
     CDS             4217..5077
                     /locus_tag="P5558_16750"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY26020.1"
                     /translation="MVERLTDEAVEQYKRDGILFPIRVFDEATAAELRGKVEALAKAE
                     GGKLSRSTNQKPHLLLPWLADLVRDPRIVEPVSAILGPNVLCWASGFFWKQPKDPAFI
                     SWHQDSTYWGLSHADITTAWVALTPSTPESGCMQVVPGTHHREQVAHKDTFAENNLLS
                     RGQEIAVEVDPKDVVDVVLQPGEMSLHHVRLFHGSEPNRSDLPRIGFAIRYIPTYIRQ
                     TTEARDSATLVRGTDEFGHFAPEPRPRSDFDPDCVAFHKAMLESAEKILYAGAAKVKH
                     FTAAEPRPGM"
     gene            5106..6092
                     /locus_tag="P5558_16755"
     CDS             5106..6092
                     /locus_tag="P5558_16755"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HRY26021.1"
                     /translation="MQKTLAGLALGTALATFAWAQGVDAADYTIRLAHSLSATEPAHL
                     AAEFFAANVNERSGGRIEMAVFPGEQLGSGKDVNEMIRQGANVMNITDAGYLSDYVPD
                     IGILNGPYLMADYRDFQKIVDSEWLAEVDAQLQEAGIRVVSYNNLFGRRHMIADMPIR
                     TPADIEGVTVRVPPNQMWLATFEAMGARPTTVQWSEVYNALSQNVVAAAEAPLGSLWG
                     SKLHEVKDTISLTGHFTAFAHFTMSEDFFQSLPEDLQTIMLEEGKRAGEEMTRLTLEK
                     QDEYIGLFEDAGVTIVEDVDIPAFQEATASVYEAFPQWTPGLHAKIQAILAQ"
     gene            6106..6591
                     /locus_tag="P5558_16760"
     CDS             6106..6591
                     /locus_tag="P5558_16760"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY26022.1"
                     /translation="MSDGTGPRQPGRYDIAEEVVACAALLVVVAAVCWGVLTRYVTAQ
                     PAAWAGEVAGMGFAWLIFLGAAAGFKRRLHVSIDLLTNHLPAPLERALALVVQVGILV
                     FATYVTWLGVGFTIGNLDNPSPVLRLPQSIIYLAVTVGFFSIAIRQAARLVCGLREAS
                     A"
     gene            6588..7871
                     /locus_tag="P5558_16765"
     CDS             6588..7871
                     /locus_tag="P5558_16765"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY26023.1"
                     /translation="MNLLPLLVLLVLFAINLPVAFAMAMAALAFFLQGSGPPMGIYVQ
                     RLVAAADSFPLLAVPFFILVGTIMNHAGITRRLLVLAEALVGHWRGGLAHANVALSTM
                     MGGLSASANADAAMQAKMLGPEMVQRGYQPAFVAAITACSAVVTPIIPPGIGLIIYGF
                     LADVSIGRLFIGGIVPGLLICAALMIATAIVARRRGYQPVRERFVGGPELWAAVKDAG
                     FALTIPVFILVGIRFGIFTPTEAGAMTVVYATLVGSLAHGELKLRDAPAILLETVLAT
                     STVMLIICAAGAFGFYMAWERIPPQLAALLATLTDDPWLLLLFINLLLLGIGMLLEGT
                     AALILLAPILVPVVAAHGIDPVHFGLVIVVNLTIGAVTPPVGTLMYTTTTILGTSLQD
                     FVRESWPFVLALLAVLALITYVPALVLFLPNLLMG"
     gene            complement(7880..8134)
                     /locus_tag="P5558_16770"
     CDS             complement(7880..8134)
                     /locus_tag="P5558_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRY26024.1"
                     /translation="MGIEALIVFLIIGAIAGWLAGLIVKGYGFGLLGNIVVGIVGAFV
                     AGLILPAIGLAFGGGILAAIIHATIGAVILLFLIRLVKRI"
     gene            complement(8177..9082)
                     /locus_tag="P5558_16775"
     CDS             complement(8177..9082)
                     /locus_tag="P5558_16775"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRY26025.1"
                     /translation="MREPEAGWPQKTRELHNHHFDSTVWNDFAFRQGDIVIATYAKSG
                     TTWLQQIVGQLVFAGATDVPVAELSPWVDLRLPPKEVKLPALEAQTNRRFVKTHLPVD
                     ALVYSPLARYIYIGRDGRDVMWSLHNHHSKANALWYELINDTPGRVGPPIGRPPEDVQ
                     QYFADWLDGDGYPFWSYWENVRSWWAIRDLPNLLLLHFQELKDDLPGQIRRIAAFLGI
                     EIDERRFPAIVEHCGFAWMKAHAAQAAPLGGAVWEGGGESFVHRGTNGRWRHELSPEL
                     SHRYEALAERELGEACARWLATGYR"
     gene            complement(9195..10213)
                     /locus_tag="P5558_16780"
                     /pseudo
     CDS             complement(9195..10213)
                     /locus_tag="P5558_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1653 a         3607 c         3373 g         1708 t
ORIGIN      
        1 accatttcat caccctgacc tcgggccacg agaacctgat gaagctcggc aacctggtgc
       61 tctgggaggg ggcgaagccg atctcgttcc ggctggcctg agcacgcccc tcgacagcgg
      121 tcgcgccgat gcttgtcgtg cgtacgcagt ggtggtaaaa ataccggttg tcgccgatcg
      181 gctcgcgagg ggctgatccg gcgtctcctt ttctcgtgcc ggtccgcgcg cgttgcgccc
      241 tgctcgcaca cgccctggac cgcaggatgt gggagaaggc gccttggctc gagcacgact
      301 gacgctcgct tgctgcgttc tcgccatggg tctcgccccg cccgtggcgg gctcggccat
      361 cgagaaggcg ccccgctctg ccacggggcc ggtgcaggtg gaccccgatt ccggcgttca
      421 gatcagggtc ggcaaggtca cgagcgtgcg aaccgagggt gggcgcacgc gctttcgcat
      481 cgattcctgc ccctttcagc cgaccaaccc gaccttcttc gccatcgcca gcgacaaccc
      541 ggacaagctc gacatcacgc gcctcctgat ctcggcgatg atcttcgacc gggaggtcaa
      601 ggtctacaac accagcttcg gcgaggacgt cgactggtgc ccgggcacca gggtcatcgg
      661 ggatattacg gtggggaccg tccgtgccag cgactgaccc cgggacagcc cgcgcgagat
      721 cggtgctggc agcttgcgcc ctggcggcgt tcctcctgcc acccatgcac gccgacgcgc
      781 ggcgcggcat cgtgagcgag gtgacgagcg tcggcggcca gttgcgcttc tggctggccg
      841 agtgcggcag caatgccttc ttctacttca ccgccgacga cccgtcgcgc gatcaggcga
      901 agctgatcgt gctggccgcc aagttcagcg gccagccggt cgcggtgacc gccgggtgcc
      961 cggcccaggg cagcacgcgc gtcacggatc tcgagctcgt cggctattga gatccccaag
     1021 gacgggaacc ggcgatggac gcctttcgac ttcggcatct cctttcctcc accttgctgg
     1081 cggcggctct ggtggcgacg tcgccggccg ccttcgcggt caagccctac gaggaggggg
     1141 aaggcggatc gacgtcgcag cccgatctcg acgacgtgct cgaggagcac ggcaacgaca
     1201 cggaaagcag cgggaacggg ccgaccggca acaccggcac gccgcgcgcg gtgactgtcg
     1261 atggcttcgc cctggcggac gtgccgcgca ccgggccccg cctggccacc ggctatgcga
     1321 tgacgcacga gcacccgacg cacggcatgg cgttcggcgg caattacggc ttcgccgggc
     1381 acgaaggcaa ctatgtcaat ggcattcccg aggacggcta ccagaaccag ggtgcctgca
     1441 gcggctgctg cgaccatggc gagttcaagg gcaacctgat cccctacttc cagttctcgg
     1501 acatgggcag gcacgatccg cacggcggtg cccggttcga cagcttctcc caccttcgct
     1561 acagcagcga ctggatcgag aacgccctcg accccggccc cggggtgggc gcggacacca
     1621 gcatgaaaat cctggtggcg ttcgccgtcg agagcgaatc gatgtgcgag ctggtttact
     1681 acgacaatcg gggcaagggc ggcgccggtg gcgccggcta tccctgcagc gagggcgaca
     1741 gcctcgcctc gctcgtgcgg cagatcgatg cgctgaaggc ctgggcagcg gcgcacgact
     1801 gggccgagat cgcctactcg gcggccgagg cacgcgccat cgccaacgcc ggcaagctcg
     1861 cggtggtcct cggcgtggag gcggactaca cctggggggc ggaggaccgg acgttcgatc
     1921 ccgtggcacg cctgcgcgac tacagggaca tgggcgttcg caccttctat ctcgcccaca
     1981 agatcaacag ccggctcgcg ggcgcggaca tctttcgcag cccgagcgag caggacggca
     2041 agagcattcg agcacttcag ggcatcgccg gatgcttcta ctacgatgac aatgtcggcg
     2101 aatttccgct cggcagctat tgcgacaact tcagccgttg cggcgcgaat gcgttccgcg
     2161 ggccgctccc gaccgaccag tgcgccacga aggtcagcga catctcggaa gccaacctcg
     2221 cgctgtatct cctgaccggc agcatcaacg gcttcgccgt ctatccgcgg ccaccgggct
     2281 tcagcgatcc ctcgggcgga tcgcgcatgg acggcgcggt cgagaggaac aacctgtcgc
     2341 tgtcgatcga gggctcccgc gtggtcgtcg aggcgatgcg gcacggcatg atcatggatc
     2401 tgggccacgt ctcgtcgaaa gcgcggacgg acatccacga ccactcgcag gccttctaca
     2461 actacccgct caacgccctg cacaacaacc cgaacgagat gctgatcgag ccggcaccca
     2521 acgagtacga cttcgacaag tccgagcgcg actatgtcag ggaaacgggc gggatgttcg
     2581 gcctccggct cggcccggtc gacgccagga gctacgcggc atcgggcgtc accgcgaatt
     2641 gccccaagac ctcgaccgag aacgccaaga ttctcgccta cctgctcgac gagggccagc
     2701 gcgtcggcta ctcgctcgac ttcgcgacgg tcaccgaagg cacgcactcg cgcacgctcg
     2761 agaactgcgg catcgccctc ggtcacgact acatcgaccg gtacgccgcg acgggtgccc
     2821 cgaacgacac cgtcacccat ggcctggccc atatcggcaa catggaagcc tggcacgacg
     2881 agctcctgac cgtcgggctg aagcagcgct atgtcgacaa gctcgagaac cagagcgcgg
     2941 agtggttcct gcggatgtgg gagaaatccg agttcaccgc caggatgccg ccgaccgtca
     3001 tggccattct gtgagccgcg aggagggtga agcatgccca cccgccccaa gtcgcccgtc
     3061 ccctgcgcgg cagcagcgtc ggctcctcga ctgccgccgg cgatggcggt cgcttgcgcc
     3121 atcgtccttc ttgccatgcc cgccttcgcc gccaagcctt atcaggaagg ggaaggcggc
     3181 tcctcgtccc agcccggccc cggcgatttc ggggtggagc tgcccggcgg cgggcagacc
     3241 gtcgagccgc tcttcgtcac cggccgcgtc accagcctgc ggctgcagaa gctgggaaat
     3301 cccctggtct atttctccct cgatgatccc agaggctgca ccgcccatcc ggaagtctat
     3361 ttcttcgacg ggaacgaccc ggactatgcc tatgcgctca aggcgctcgt cgcatcgcgg
     3421 ctcagcggcc gcgaggtgaa gctcgaaggg atgaagggct gccccggccc accggtcggc
     3481 atcgacgcca gcgccgtcca catcatcgaa tcgtcgcccg aggccggacc ggtcgccgcg
     3541 cccgccgacg ggcttttcgg cgaggtcacc aacctcatga tcaaggaggg gtcggtctat
     3601 ttccgcctct cggcttgcag cggcagcgcc ccctatttca ccttcgccgc cgacctgccg
     3661 ggcggcttcg cgctcctgct cgcggcgcag agcgccacgc ggccggtgcg ggtggagacg
     3721 acgatcggct cgaccagcac ctgcccgacg gccggcgggg gcgacgtcga attgcaggga
     3781 ggctcgttgt ggctgctcta ggaggctcct ctggaactgc cctggccgcc gccgccatcg
     3841 cggcctgcct cgggccgtcg atcgcccatg cgctcgacgg cggcgtgaag gagctctgga
     3901 ccgaaagcgg ccgagtgcgc ttcatgctcg acacctgcgc caccgacgtg gacttcgagc
     3961 tggcggccac ggatgccgac gcctccatgg cctatgacat cctgctctcg accctgctcg
     4021 cccgtggcag ggtcaggatc tacgatcggc aaggcgggcc gatcgcctct tgcccgcaac
     4081 gggacacgca gatcggcagc gtccgcgccc tgccctagct gccctcgatg gcgtcgccgc
     4141 cggaccgctt gtcgcgcggc gggccgctgc ctagagtgcg cgccaacgac tttcgggtga
     4201 gcaagggaga agcttcatgg tcgagcggct gacggacgag gccgtcgagc agtacaagcg
     4261 tgacggcatc ctctttccga tccgcgtctt cgacgaggcg acggcggcgg agctgcgcgg
     4321 caaggtcgag gcgctggcga aggcggaggg cggcaagctc tcgcgctcga ccaaccagaa
     4381 gccgcatctc ctgctgccct ggctcgccga cctcgtgcgc gacccgcgca tcgtcgagcc
     4441 ggtttcggcg atcctcggcc ccaacgtcct ctgctgggcc tcgggcttct tctggaagca
     4501 gcccaaggac ccggccttca tctcctggca ccaggattcg acctattggg gcctgagcca
     4561 cgccgacatc acgaccgcct gggtggcgct gacgccgagc acgccggaga gcggctgcat
     4621 gcaggtggtg ccgggcacgc atcaccgcga gcaggtggcg cacaaggaca ccttcgccga
     4681 gaacaacctc ttgagccgcg gccaggagat cgcggtcgag gtcgacccca aggacgtggt
     4741 cgacgtggtg ctgcagccgg gcgagatgtc gctccaccat gtgcgcctct tccacggctc
     4801 cgagcccaac cgctcggatc tgccccggat cggcttcgcc atccgctaca tcccgacata
     4861 catccgccag accaccgagg cgcgcgacag cgccacgctg gtccgcggca ccgacgaatt
     4921 cggccatttc gctcccgagc cgcggccgcg gtccgacttc gacccggact gcgtcgcctt
     4981 ccacaaggcg atgctcgaga gcgccgagaa gatcctctat gccggtgccg ccaaggtgaa
     5041 gcatttcacc gcggccgagc cccggcccgg catgtaacga aaagaaaccc gagggaggca
     5101 cgaccatgca aaagacactc gccgggctgg cgctcggcac ggcgctcgcg accttcgcct
     5161 gggcgcaggg agtggatgcg gccgactaca ccatccgcct ggcgcactcg ctctcggcga
     5221 ccgagcccgc gcatctggcg gccgagttct tcgccgccaa tgtcaacgag cgctcgggcg
     5281 ggcggatcga gatggcggtc ttcccgggcg agcagctcgg ctccggcaag gacgtcaacg
     5341 agatgatccg ccagggggcc aacgtgatga acatcaccga cgccggctat ctctccgact
     5401 acgtgccgga catcggcatc ctcaacggcc cctatctgat ggccgactat cgcgacttcc
     5461 agaagatcgt cgattccgag tggctcgccg aggtcgacgc ccagctccag gaggccggca
     5521 tccgcgtcgt ctcctacaac aacctcttcg gccggcggca catgatcgcc gacatgccga
     5581 tccgcacgcc ggccgacatc gagggggtca ccgtccgggt gccgccgaac cagatgtggc
     5641 tcgccacctt cgaggcgatg ggggccaggc ccacgaccgt ccagtggtcg gaggtctaca
     5701 atgcgctctc gcagaacgtg gtggccgcgg ccgaggcgcc cctgggctcg ctctgggggt
     5761 cgaagctgca cgaggtgaag gacaccatct cgctgaccgg gcacttcacc gccttcgccc
     5821 atttcaccat gagcgaggac ttcttccagt cgctgccgga ggacctgcag acgatcatgc
     5881 tggaggaggg caagcgggcc ggcgaggaga tgacgcggct cacgctggag aagcaggacg
     5941 agtatatcgg cctcttcgag gatgccggcg tcaccatcgt cgaggacgtc gacatccccg
     6001 ccttccagga ggcgacggcc agcgtctacg aggcgttccc gcaatggacg ccgggtctgc
     6061 acgccaagat ccaggcgatc ctggcccaat gagcgcgccc ggggagtgag cgacgggacc
     6121 gggccccggc agcccggccg ttacgacatc gccgaggagg tggtggcctg cgccgcgctc
     6181 ctggtcgtcg tcgcggccgt ctgctggggg gtgctcaccc gctatgtcac cgcccagccg
     6241 gccgcctggg ccggcgaggt ggccggcatg ggcttcgcct ggctgatctt cctcggtgcc
     6301 gccgccggct tcaagcgcag gctgcatgtc agcatcgacc tcctgaccaa ccatttgccg
     6361 gccccgctcg agcgggcgct ggcgctggtc gtgcaggtcg gcattctcgt cttcgccacc
     6421 tatgtgacct ggctcggcgt cggcttcacc atcggcaatc tcgacaaccc gagccctgtc
     6481 ctgcgcctgc cgcagtcgat catctacctc gcggtgacgg tgggcttttt ctccatcgcc
     6541 atccgtcagg ccgcccggct ggtttgtggt ctgcgggagg cgagcgcttg aacctcctgc
     6601 cgctcctcgt gctgctcgtc ctcttcgcca tcaacctgcc cgtcgccttc gccatggcga
     6661 tggccgccct cgccttcttc ctccagggca gcggcccgcc catgggcatc tatgtccagc
     6721 ggctggtcgc ggccgccgac agcttccccc tgctcgccgt gcccttcttc atcctcgtcg
     6781 gcacgatcat gaaccatgcc ggcatcaccc gccggctcct ggtcctggcc gaggcgctgg
     6841 tcggccactg gcggggcggc ctggcgcacg ccaatgtggc gctttcgacg atgatgggcg
     6901 ggctctccgc ctcggccaat gccgatgccg ccatgcaggc gaagatgctc gggccggaga
     6961 tggtccagcg cggctaccag ccggcctttg tcgccgccat caccgcctgc tcggccgtgg
     7021 tgacgccgat catcccgccg ggcatcgggc tgatcatcta cggctttctc gccgacgtct
     7081 cgatcggccg gctcttcatc ggcggcatcg tcccgggcct gctgatctgc gcagcactta
     7141 tgatcgccac cgccatcgtc gcaagacggc ggggctacca gccggtgcgc gaacgcttcg
     7201 tcggcgggcc ggagctctgg gcggccgtca aggatgccgg cttcgcgctg accatcccgg
     7261 tcttcatcct ggtcggcatc cgcttcggca tcttcacgcc gaccgaggcc ggggcgatga
     7321 ccgtggtcta tgccacgctg gtcggcagcc tcgcgcatgg cgaattgaag ctcagggacg
     7381 cgcccgcgat cctgctcgag accgtgctcg ccacctcgac cgtgatgctc atcatctgtg
     7441 cggccggcgc cttcggcttc tacatggcct gggagcgcat cccgccgcag ctcgcggccc
     7501 tgctcgcgac gctgaccgac gatccctggc tgctgctcct cttcatcaac ctcctgctgc
     7561 tcggcatcgg catgctgctc gagggcacgg cggccctcat cctgctggcg ccgatcctcg
     7621 tgccggtggt ggccgcacac ggcatcgatc ctgtgcattt cggcctggtc atcgtcgtca
     7681 acctgaccat cggcgcggtg acgccgccgg tcggcacgct gatgtacacg acgaccacca
     7741 tcctcggcac gtcgctccag gacttcgtcc gggaatcctg gcccttcgtc ctggcgctgc
     7801 tcgccgtcct ggcgctgatc acctacgtgc cggcgctggt gctcttcctg ccgaatctgc
     7861 tcatgggttg agcctgggct cagatgcgct tcacgaggcg gatcaggaag agcaggatca
     7921 cggcgccgat cgtggcatgg atgatggcgg ccaggatgcc gccgccgaag gcgaggccga
     7981 tcgccggcag gatcagcccg gcgacgaagg cgccgacgat gccgacgacg atgttgccga
     8041 gcaggccgaa gccgtagccc ttgacgatca gcccggcgag ccagccggcg atggcgccga
     8101 tgatgaggaa gacgatcaaa gcctcgatgc ccaagacgtt tctcccctcg cgttgtcgac
     8161 cagacccaca gtaccgctag cgataccccg tcgccagcca ccttgcgcag gcttcgccca
     8221 gctcgcgctc ggccagcgct tcgtagcggt ggctgagctc ggggctcagc tcatggcgcc
     8281 accggccgtt cgtgccccga tggacgaagg actcgccgcc gccttcccag accgcaccgc
     8341 cgagcggggc ggcctgggcg gcatgcgcct tcatccaggc gaagccgcaa tgctcgacga
     8401 tggcggggaa ccggcgctcg tcgatctcga tcccgagaaa cgcggcgatc cgccggatct
     8461 ggcccggcag gtcgtccttc agctcctgga agtgcagcag cagcaggttg ggcaggtcgc
     8521 ggatcgccca ccaggagcgg acattctccc agtatgacca gaacgggtag ccgtcgccgt
     8581 cgagccagtc cgcgaaatac tgctgcacgt cttccggtgg cctgccgatc ggcggcccga
     8641 cccggccggg cgtgtcgttg atgagctcgt accagagcgc gttggccttg ctgtggtggt
     8701 tgtggaggct ccacatcacg tcgcggccgt cgcgaccgat atagatgtac ctggcgagcg
     8761 gcgagtagac gagcgcgtcg accggcagat gggtcttgac gaaccgccgg ttggtctgcg
     8821 cctcgagcgc cgggagcttc acctccttgg ggggcaggcg cagatcgacc cagggcgaca
     8881 gctcggccac cgggacgtcc gtcgcacccg cgaagacgag ctggccgacg atctgctgca
     8941 gccaggtcgt ccccgacttg gcataggtgg cgatgacgat gtcgccctgg cgaaaggcga
     9001 aatcgttcca gacggtcgaa tcgaaatgat ggttgtgcag ctcccgagtc ttctggggcc
     9061 agccggcctc cggctcacgc acgactcttc tccttgttct tgtcgccacg agtgctcgcc
     9121 accccggcaa tgctagtcgc gtccgacacg gccgtcgaga gaccgcggca acgatccgct
     9181 agagcagaat tcgatcaagt agcggcatat ccggcgtgac ggaggtagtt tcggcattcc
     9241 tggggcgtga tttcgtcgag cagggtgccg atgcgtcgcc agaggctgtc gaccgtccgc
     9301 tcggcggctc tgcgcaggcc tgcctcgagc ttggcgaaca gcatctcgat cggattgagg
     9361 tcgggcgagt acggcggcag gaagcgcagc tcggcgccgc gggcctcgat cgcgcgccgc
     9421 gcggcgtcac ccttgtggct gccgagattg tcgaccacga cgatgtcgcc gggttgcagg
     9481 gtcggtgcga ggaactgctc gacataggcg tcgaaggtgg cgccgttgat cggtccgtcg
     9541 agcaccaggg gcgcctcgac ccgatcgacc cgtagcgccg cgagaaaggt cgtcgtcttc
     9601 cagtgcccat gattggtcag cagaccaatc atccgattca tggaatgacg agggccagca
     9661 ggccctcgag ggaggcaccc ggccgatcag gcgtgagcca cgcgccgtac ggccgcgaag
     9721 gcgcgccatg ttggtcttga ccccataggt atcgaggggg gtctcgtcga tgaagacgag
     9781 cttgcccgaa gcgagctcga cctgaccctc gagccaggcc ttgcgcgcct tcgtcacgtc
     9841 cgaccgctcc tgctcgctgg cgtggaccgt tttttttaaa gctcaggccc tccttggcga
     9901 tgaaccgcca aagagcgccg tggctggtga tgatcccctg ggtggcgagc atcgcccgca
     9961 tctcatcgag cgtcgtgtcg ggccgtgccg cgatcatctc cagcagaagc tcgcgatgcc
    10021 gctcgagcga cgagcggcga tccccaccca tcgccagcgg cgcaacccgc ccctccgcgt
    10081 gccaggccgc cagccagcga acggcggcgc tggcgctcac gccgaagcgc ctggcagcgt
    10141 gccgtcgcga tgcaccgctc gccacggcct cgatcacgcg ctggcgcaag tccttcgata
    10201 gcggtcccgg catcaaggct ggcccccttt aggctctgaa cccgattaac atattgaata
    10261 gttgttattt ttatgattct tggtgttcaa gagaacgcta aggaggtcga gaatgacgag
    10321 ctatcccttc tggctgaacg a
//
LOCUS       DAOTFI010000178        10288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7708_length_10288_cov_6.697352, whole genome shotgun sequence.
ACCESSION   DAOTFI010000178 DAOTFI010000000
VERSION     DAOTFI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7708_length_10288_cov_6.697352"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="P5558_16785"
     CDS             <1..866
                     /locus_tag="P5558_16785"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRY26026.1"
                     /translation="LLTGGVHGDEYEGPIAVSGLAQSLQPADVQGRVILMSAVNVPAV
                     LNNTRLSPVDNRDMNRCFPGDPKGTFSEMVAHFMDSHILPMVDVSVDLHTAGHSGDSI
                     PSTNMHHIADPALMQRTLDAAAAFGAPYNVVFGGVDEGATFTSCVERRGKLSLGTELG
                     GWGRVNVEGVRIGKRGCLNILKHLGVIEGTPETAQKDGSAGTRHMMVAGRDCYAFAPT
                     GGIFEPVHLVGEEVRAGGPAGWLHFVEDVDHPPLEVRYASGGILWMSAGPGRVARGDT
                     VAVVMSDYRAS"
     gene            888..2375
                     /locus_tag="P5558_16790"
     CDS             888..2375
                     /locus_tag="P5558_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRY26027.1"
                     /translation="MPRILMLDCKQEISSFNPLPSEYANFRITYGNSFYEHRGLNTEL
                     GGAFSVFDERDDVVTVPTIAARAGSAGPLSAKGWQKLKAEIIAAIEPVAGEVDAVYAS
                     LHGAMGSVAELDPEGSLLKAVRRMVGPDVPIVISLDLHGILTERMLKQVNGVVVYHTY
                     PHVDFGDTGARAARLLLRILDEGIRPVIARVVIPTLVRGNELITAKGCYGDLIRECQR
                     LERDGTALAAAIMIGNPFTDVPELCTQVLVSTDDDKATAEAEAIRLAREFWPQRHRMQ
                     GVLIPLDRAIAQARTMQGPLIFTDAADATSSGATGDSNAIIRALSEARYKKRVLAQIV
                     DPEAAKAAHAAGVGATVKVTLGGAIDTRRYSPMAVEARVKLLMDGEARLETMKTPLEA
                     GPVAVLTFANFTIVVLSTSVSLFDRAMFYAAGCNPRDYDLVVVKSPHCEPHMFDDWAE
                     KNFNIDAPGATSADLASLGHTICARPMYPMEPDTTFEPKAVIYGR"
     gene            2378..3889
                     /locus_tag="P5558_16795"
     CDS             2378..3889
                     /locus_tag="P5558_16795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY26028.1"
                     /translation="MGARPNILFLFSDQHAQRIAGCYGDPLGATPNIDRLAARGVTFD
                     NAYCASPICTPSRMSLLTGRWPHQQSCWTLQDTLASDLPTFAHALGAAGYRTILVGRM
                     HSIGPDQLHGYAERDIGDCGPNWLGVERQKLGVLGGTQGPSPPDAEGRALSISRSGSG
                     QSGYEVVDDATTEAAIARLDELGAAGEDAPPFCLTVGFILPHCPFVARGPDYARFAGR
                     VGPPRLPPPPADREHPWHAKWRAHAATVDADPDDVIRARTAYWGLVSALDAKIGRILD
                     RLEVLGLSENTLVVYASDHGEQIGEHGLWWKNTFYEDSVKVPLVMAAPGLLPEGGRCG
                     RIVNLIDIGATLIEAAGAPPLPRSHGRSLLEIARAPDGPWVDETWSEYVTDLLSVWTG
                     PEAVCQRMLRTARWKYVHMEGYRAQLFDMAADPDELNDLGADPAYAEVRATLSARVLA
                     GWDPDAIRREVDARCEEKRLLEAWGSRTRPKSTAQYLIRDEDSWLDDLPAQRL"
     gene            complement(3869..>4085)
                     /locus_tag="P5558_16800"
     CDS             complement(3869..>4085)
                     /locus_tag="P5558_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26029.1"
                     /translation="ADPEEELNIYLTPRDDGGFERFKHYPDQAPVIADLCERMAAEAA
                     RIGDAEGLDIIGEVQADIAPRLKALGR"
     assembly_gap    4086..4185
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4186..5301)
                     /locus_tag="P5558_16805"
     CDS             complement(<4186..5301)
                     /locus_tag="P5558_16805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY26030.1"
                     /translation="MSTRPNIVLVLTDQQRRDTLGTYGNRFTETPAADAMAAAGMTFE
                     NAYTPWPVCTPARGTMWTGVYPHAHGLIENLYGVDDAFASHCTSHATVWTQLQRAGYL
                     TAHFGKWHLGEKHPPFFDVWEESFNSRKGHWLDGRLDGKYRPDRQTDAATAFIARQQD
                     AEQPFAMVLSFYPPHDPYSAPARFFEPYRGRGVPFAGYYAAVSALDHNLGRVRQALRD
                     AGLAERTIVIFYSDHGDTFWYRPEGEHKFVCHDDAIRIPFLAEGPGIAEGSRNAAAIG
                     LQDLTPTMLEIAGAPLPEGLHGRSILPLLRGEADDGRPADAYVENITHKSRIHQRAIR
                     TDRWKLIASANGAHELFDLEADPEEELNIYLTPRDDGG"
     gene            complement(5298..6293)
                     /locus_tag="P5558_16810"
     CDS             complement(5298..6293)
                     /locus_tag="P5558_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26031.1"
                     /translation="MSAAGPLVEVEDLAKEFLAGASGFMKARRPIRAVDGVSFSIDRQ
                     ETLGLVGESGCGKTTTGRLTLRLIEPSAGSIRFAGTDILALEPAALRALRRDMQIVFQ
                     DPFGALNPRMTAGELIVEPLVVHGIGDVTARERKLRRLLDMVGLAAHHAGRFPHEFSG
                     GQRQRVCIARTLALDPAFVVCDEAVSALDVSVQAQILNLLHDLKAELGLTYLFISHDL
                     GVVRHISDRVAVMYLGQIVELGTREAIFAAPAHPYTRALLQSVPSRAKGRKSFFTIEG
                     DVPSPANPPPGCRFHTRCPLVFDRCRVEAPRLRPATAPGHIAACHLVEPAIEENT"
     gene            complement(6290..7288)
                     /locus_tag="P5558_16815"
     CDS             complement(6290..7288)
                     /locus_tag="P5558_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26032.1"
                     /translation="MTAVPGQPAPLLEVEGLTVSIGGIVILDDLSFRVAPGEILGIVG
                     ESGSGKSMTALSIMRLLPDGAEVSATQLRLEGEDILGAREARLEELRGRAMGMIFQEP
                     MTALNPVMTVGEQIMETVQRHLKVPRREARERAIEALRRVGIPSPEQRVDDYPHRLSG
                     GMRQRVMIAMALVCRPRLLIADEPTTALDVTIQAQILDLMLGLQDEYRMGIILISHDL
                     GVVSAFTDRVLIMYMGQVLEDARADDVFSDPKHPYTEGLLASIPPVDRDMARLEAIPG
                     AIPPISNLPPGCRFEPRCAHARPVCTSAVPALVETGPAHRAACIRNTGYRFTGAGR"
     gene            7396..8838
                     /locus_tag="P5558_16820"
     CDS             7396..8838
                     /locus_tag="P5558_16820"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY26033.1"
                     /translation="MPNVVVVLADQMRRDALGCAGNANLRTPNLDRLAERGVRFTAAC
                     ATFPACVPFRFSLLTGEYAHSRNVPALGYRLSPAERTLGDALEAAGLATAYIGKWHLY
                     SAYGVSGGMSLSQASRTPIPASHRRGFDHWQGFELRNDFADTWIFEGDDPLPRHLPGH
                     QTDALFDLALDYLERKRPASQPFFLMLSIEAPHPPFMATAETLARVQARGPLQHRINV
                     DLGAIRFFPPEWHEATGPAGRIDPADPASVKRVFEANMQAYYAMIEQIDDNMGRLRAT
                     LERQGLADDTITVFLADHGELGGSHGLLGKAEPWEESIGVPLIVAGPGVPPGREAAMP
                     VHTEDLFSTFVGLAGGSERPAPPRVDLAPFIRGEAPPPERPGVMLEFVTETRANRSYY
                     DKRWRGIRGPRHKYTVLGDMTGALPWQLFDLEADPFELRNLLAEPGHEPVAAAMHRAL
                     IGLLDAAADDFALAPAFGSPARHAVPERVS"
     gene            complement(8811..9560)
                     /locus_tag="P5558_16825"
     CDS             complement(8811..9560)
                     /locus_tag="P5558_16825"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY26034.1"
                     /translation="MPTASATPAKPGLSGLAPLAAKTLVNQVVDAIVEASARGVFLPG
                     DRIVEAEVARALEVSRIPVREALRLLESQGVVVSERYRGMRLMSVDIDRLEKILKVRL
                     ALEKLAGAEIMALRQGQKGFLAPLDEMLARMHRAADADDKFLVASLDTEFHMLLCRLS
                     GNDTLLRCWESLSRQLTIIFGLSTLKKSLKSIVAEHDEVIAALRTGDQATFDRLMEVH
                     ILEYSRAVDYAKLVEQLRKLNQRQETRSGTA"
     gene            9759..>10288
                     /locus_tag="P5558_16830"
     CDS             9759..>10288
                     /locus_tag="P5558_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26035.1"
                     /translation="MAGTVALTSALMATSALAQVMGGDVVVVQGSNPPSLDAMSTSSQ
                     ASRNVNMNMYETLYGFSDAIQPIPILAEGVEISDDGLTYVFTLRQGVKFHNGDEMKAS
                     DVKASLERYRKVGATGNLLEPVTNIEVTGDYEVTFTMAKATPTFLEAFSSPRAPAVII
                     PEEDAEAEAGKTSLIGT"
BASE COUNT         1559 a         3607 c         3468 g         1554 t
ORIGIN      
        1 tgctgctcac cggcggcgtg cacggcgacg aatacgaggg gccgatcgcc gtctcgggcc
       61 tcgcacagag cctgcagccc gcggacgtcc agggccgggt gatcctgatg tccgccgtca
      121 atgtcccggc cgtgctcaac aacacccgcc tctcgcccgt cgacaaccgc gacatgaacc
      181 gctgcttccc gggcgacccg aagggcacgt tcagcgagat ggtggcgcat ttcatggaca
      241 gccacatcct gcccatggtc gacgtctcgg tcgacctgca cacggccggc cattcgggcg
      301 attcgatccc gtcgaccaac atgcaccaca tcgccgatcc cgccctgatg cagcgcacgc
      361 tggatgccgc cgccgccttc ggcgccccct acaacgtcgt cttcggcggc gtcgacgagg
      421 gcgccacgtt cacttcctgc gtcgagcggc ggggcaagct ctcgctcggc acggagctcg
      481 gcggctgggg ccgcgtcaat gtcgagggcg tgcgcatcgg caagcgcggc tgcctgaata
      541 tcctcaagca cctcggcgtc atcgagggga cgccggagac ggcccagaag gacggcagcg
      601 ccggcacgcg ccacatgatg gtcgcgggcc gcgactgcta cgccttcgcc ccaaccggcg
      661 gtatcttcga gccggtccat ctggtcggcg aggaagtccg ggccggcggg cccgccggct
      721 ggctgcactt cgtcgaggac gtggaccacc cgcccctcga ggtccgctat gcctcgggcg
      781 gcatcctctg gatgtcggcc ggccccggcc gggtcgcgcg cggcgacacg gtggccgtcg
      841 tcatgtcgga ttaccgggcg tcctgatcgc caacggagaa acgggccatg cccagaatat
      901 tgatgctcga ctgcaagcag gagatctcct ccttcaaccc gctgccgtcg gaatacgcga
      961 acttccgcat cacctacggc aacagcttct acgagcatcg cgggctgaac accgagctcg
     1021 gcggcgcctt ctcggtcttc gacgaacgcg acgacgtggt gacggtgccg accatcgccg
     1081 cccgggcggg cagcgccggg ccgctctcgg ccaagggctg gcagaagctc aaggcggaga
     1141 tcatcgcggc gatcgagccc gtggccggcg aggtcgacgc ggtctacgcc tcgctgcacg
     1201 gggcgatggg ctcggtggcg gagctcgacc cggagggcag cctcctcaag gcggtccgcc
     1261 gcatggtcgg gccggacgtg ccgatcgtca tctcgctcga cctgcacggc atcctcaccg
     1321 agcggatgct gaagcaggtg aacggcgtcg tggtctacca cacctacccg catgtcgatt
     1381 tcggcgacac gggtgcgcgg gcggcgcggc tgctgctccg tatcctcgac gagggcattc
     1441 gcccggtgat cgcccgggtg gtgatcccga ccctggtgcg cggcaacgag ctgatcaccg
     1501 ccaagggctg ctacggcgac ctgatccgcg aatgccagcg cctcgagcgc gacggcacgg
     1561 ccctcgccgc cgccatcatg atcggcaacc ctttcaccga cgtgccggag ctctgcacgc
     1621 aggtcctggt ctccaccgac gacgacaagg ccaccgccga ggccgaggcc atccggctgg
     1681 cccgggagtt ctggccgcag cgccaccgca tgcagggcgt tctgatcccg ctcgaccgcg
     1741 cgatcgccca ggcgcggacc atgcagggcc cgctcatctt caccgacgcc gcggatgcga
     1801 cctcgtccgg tgcgaccggc gattccaacg ccatcattcg tgccctgagc gaggccaggt
     1861 acaagaagcg cgtgctggcg cagatcgtcg atccagaggc ggcgaaggcg gcgcacgcgg
     1921 cgggtgtggg cgccacggtg aaggtgacgc tcggcggggc gatcgatacc aggcgctatt
     1981 cgccgatggc ggtcgaggcg cgggtcaagc tgctcatgga cggcgaggcc aggctcgaga
     2041 ccatgaagac gccgctcgag gccggccccg tggcggtgct gaccttcgcc aatttcacca
     2101 tcgtcgtgct ctcgacctcg gtcagcctct tcgaccgggc gatgttctac gccgccgggt
     2161 gcaacccacg cgactacgac ctcgtcgtcg tgaagtcgcc ccattgcgag ccgcacatgt
     2221 tcgacgactg ggccgagaag aacttcaaca tcgacgcccc cggcgccacc tcggccgatc
     2281 tcgccagcct cggccacacc atctgcgccc ggcccatgta cccgatggag cccgacacga
     2341 ccttcgagcc gaaggccgtc atctacggcc gctgagcatg ggcgcgcggc ccaacatcct
     2401 cttcctcttc tccgaccagc acgcgcagcg gatcgccggc tgctacggcg accccctcgg
     2461 cgccacgccc aacatcgacc ggctggcggc gcgcggcgtc accttcgaca acgcctattg
     2521 cgcgtcgccc atctgcacgc cgagccggat gtcgctgctc accggccgct ggccacatca
     2581 gcagagctgc tggaccctgc aggacaccct ggcctcggac ctgcccacct tcgcccatgc
     2641 gctgggtgct gccggctatc gcaccatcct ggtcgggcgg atgcattcca tcggcccgga
     2701 ccagctccac ggctacgccg agcgcgacat cggcgattgc gggccgaact ggctcggcgt
     2761 cgagcggcag aagctgggtg tgctgggcgg cacccagggg ccgagcccgc ccgatgccga
     2821 gggccgcgca ctcagcatca gccgctccgg gtccggccag tccggctacg aggtggtcga
     2881 cgacgccacg accgaggccg ccatcgcccg gctcgacgag cttggtgcgg caggcgagga
     2941 tgccccgccc ttctgcctga cggtgggctt catcctgccg cactgcccgt tcgtggcacg
     3001 gggccccgac tatgcccggt tcgcgggccg cgtcggcccg ccccgcctgc ccccgccgcc
     3061 ggccgaccgg gagcatccct ggcacgccaa gtggcgcgcc catgccgcca ccgtagatgc
     3121 cgaccccgat gacgtgatcc gcgcccggac ggcctattgg ggcctggtct cggccctcga
     3181 cgccaagatc ggccgcatcc tcgaccggct ggaggtcctg ggactcagcg agaacacgct
     3241 cgtcgtctac gcgtcggacc acggcgagca gatcggcgag cacgggctct ggtggaagaa
     3301 caccttctac gaggattccg tgaaggtgcc gctggtgatg gccgccccgg gcctgttgcc
     3361 agaaggcggg cgctgcggcc ggatcgtgaa cctgatcgac atcggcgcca cgctgatcga
     3421 ggccgccggc gccccgcccc tgccccgcag ccatggccgg agcctgctcg agatcgccag
     3481 ggcaccggac gggccctggg tcgacgagac ctggagcgaa tacgtcaccg acctcctctc
     3541 cgtctggacc ggccccgagg ccgtctgcca gcgcatgctg cgcaccgcgc gctggaaata
     3601 tgtccacatg gagggctacc gcgcgcagct cttcgacatg gcagccgacc cggacgagct
     3661 gaacgacctc ggcgccgatc cggcctacgc tgaagtgcgc gccacgctct cggcccgcgt
     3721 gctcgccggc tgggacccgg acgcgatccg ccgcgaggtc gatgcccgct gcgaggagaa
     3781 gcgcctgctc gaagcctggg gcagccggac gaggcccaag agcaccgcgc agtatctcat
     3841 ccgcgacgag gacagctggc tcgacgacct accggcccag cgcctttagc ctgggtgcga
     3901 tgtcggcctg cacctcgccg atgatgtcga gcccttccgc atcgccgatc cgcgccgcct
     3961 cggcggccat gcgttcgcac aaatccgcga tgaccggcgc ctgatccgga tagtgcttga
     4021 accgctcgaa gccgccatcg tcgcgcggcg tgaggtagat gttcagctcc tcctcggggt
     4081 cggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccgc catcgtcgcg
     4201 cggcgtgagg tagatgttca gctcctcctc ggggtcggcc tcgaggtcga agagctcgtg
     4261 cgccccattg gccgaggcga tcagcttcca gcggtcggtg cggatggcgc gctggtggat
     4321 gcggctcttg tgggtgatgt tctcgacata ggcatccgcc ggccgaccgt cgtccgcctc
     4381 gccccggagc agtggcagaa tgctccggcc atgcagcccc tccggcagcg gggcgccggc
     4441 aatctccagc atggtcggcg tcaggtcctg cagcccgatc gcggcggcgt tgcgcgagcc
     4501 ctcggcaatg cccggcccct cggccaggaa ggggatgcgg atggcgtcgt catggcagac
     4561 gaacttgtgc tcgccctccg gccgatacca gaacgtgtcg ccatggtcgg agtagaagat
     4621 gacgatcgtg cgctccgcga gccccgcgtc gcgcagcgcc tgccgcaccc ggccgagatt
     4681 gtggtcgagc gcgctgaccg ccgcgtagta gccggcgaac ggcacgcccc gcccccgata
     4741 gggctcgaag aagcgggccg gggccgaata gggatcgtgc ggcggataga agctgagcac
     4801 catggcgaag ggctgctcgg cgtcctgctg ccgggcgatg aaggcggtgg ccgcatcggt
     4861 ctggcggtcg ggacgatatt tgccgtcgag cctgccgtcg agccagtggc ccttgcgcga
     4921 attgaacgac tcctcccaga cgtcgaagaa gggcggatgc ttctcgccca ggtgccactt
     4981 gccgaaatgc gccgtcaggt agcccgcccg ctggagctgc gtccagacgg tcgcgtggct
     5041 ggtgcagtgc gaggcgaagg cgtcgtcgac gccgtagagg ttctcgatca gcccgtgggc
     5101 atgcggatag acgccggtcc acatcgtgcc gcgcgccggg gtgcagaccg gccagggcgt
     5161 gtaggcgttc tcgaacgtca tgccggcagc ggccatggcg tcggccgccg gcgtctcggt
     5221 gaagcggttg ccgtaagtgc cgagcgtgtc ccggcgctgc tggtcggtga ggacgaggac
     5281 gatgttgggg cgggtgctca tgtgttttcc tcgatggccg gctcgaccag atggcaggcg
     5341 gcgatgtggc ccggcgccgt cgccgggcga agccgcggcg cctcgacccg gcagcggtcg
     5401 aagaccagcg ggcagcgggt gtggaagcgg cagccgggcg gcgggttggc cgggctcggc
     5461 acgtcgccct cgatggtgaa gaaggatttt ctgcctttgg cccgcgacgg cacggattgc
     5521 agcagcgccc gcgtataggg gtgtgcgggt gccgcgaaga tcgcttcccg cgtgccgagc
     5581 tcgacgatct gcccgagata catgaccgcc acccggtcgg agatgtggcg gaccaccccc
     5641 aggtcatggc tgatgaagag ataggtgagg ccgagctccg ccttgaggtc gtgcagaaga
     5701 ttgaggatct gcgcctgcac cgagacgtcg agcgccgaaa ccgcctcgtc gcagacgacg
     5761 aaggcggggt cgagggcgag cgtgcgggcg atgcagaccc gctgccgctg cccccccgag
     5821 aactcgtgcg ggaaccgccc ggcatggtgt gccgcgaggc ccaccatgtc gagcagccgg
     5881 cgcagcttcc gctcgcgggc ggtgacgtcg ccgatgccgt gcacgacgag cggctcgacg
     5941 atgagttcgc ccgccgtcat gcgcgggttc agggcaccga acgggtcctg gaagacgatc
     6001 tgcatgtcgc ggcgcaaggc ccgcagcgcc gccggctcga gagcgaggat gtcggtgccg
     6061 gcgaagcgga tggagccggc gctcggctcg atcaggcgaa gggtgagccg gcccgtcgtc
     6121 gtcttgccgc agcccgattc gccgacgagg ccgagcgtct cctgccggtc gatggaaaag
     6181 ctgacgccgt cgaccgcccg gatcggcctg cgcgccttca tgaagccgga cgcaccggcg
     6241 aggaactcct tggcgagatc ctcgacctcg acgagcgggc cggccgcgct catcgccctg
     6301 ccccggtgaa gcggtagccg gtgttgcgga tgcaggccgc acggtgcgcc gggccggtct
     6361 cgaccagcgc cggcacggcg ctcgtgcaga ccgggcgggc atgagcgcag cggggctcga
     6421 accggcagcc gggcggcagg ttcgagatcg gcgggatggc gccgggaatg gcctcgagcc
     6481 gggccatgtc gcggtcgacc ggcgggatgg aggcgagcag gccctcggtg taggggtgct
     6541 tcgggtcgct gaagacatcg tcggcccggg catcctccag cacctggccc atgtacatga
     6601 tgagcacccg gtcggtgaac gccgagacga cgccgagatc gtggctgatg aggatgatcc
     6661 ccatcctgta ctcgtcctgc aggccgagca tcaggtcgag gatctgcgcc tggatggtga
     6721 cgtcgagggc ggtggtcggc tcgtcggcga tcaggaggcg tggccggcag acgagcgcca
     6781 tggcgatcat cacccgctgc cgcatgccgc ccgagaggcg gtgcgggtag tcgtcgacgc
     6841 gctgctcggg cgacgggatg ccgacccgcc gcagcgcctc gatggcccgc tcccgcgcct
     6901 cgcgcctggg caccttcagg tggcgctgca ccgtttccat gatctgctcg cccacggtca
     6961 tgaccgggtt gagcgctgtc atcggctcct ggaagatcat gcccatcgcc ctgccacgca
     7021 gctcctcgag ccgggcttcc ctggcgccca ggatatcctc gccctcgagt cggagctgtg
     7081 tcgcgctcac ctcggcgcca tcgggcagaa gccgcatgat cgagagcgcc gtcatgctct
     7141 tgccgctgcc ggattcgccg acgatgccga ggatctcgcc gggtgccacc cgaaaggaga
     7201 ggtcgtcgag gatgacgatg ccgccgatcg acaccgtcag gccctcgacc tcgagcagcg
     7261 gtgctggctg acctgggacg gcggtcacga cggggctggc ctcctcgggc gctggtgggc
     7321 tctctccgac gaatggttgt atacgaaaat acgatttgca agtttgctga cgcggtccga
     7381 accggggaga cgtggatgcc gaatgtcgtc gtcgtgctgg ccgaccagat gcgccgggat
     7441 gccctgggct gcgccggcaa cgccaacctg cgcacgccca atctcgaccg gctcgccgag
     7501 cggggcgtgc gcttcaccgc ggcctgcgcc accttcccgg cctgcgtccc cttccgcttc
     7561 tcgctgctca ccggcgaata cgcgcattcg cgcaacgtgc ctgccctggg ctaccggctc
     7621 tcgccggccg agcgcacgct cggcgacgcg ctcgaggcgg caggcctcgc caccgcctat
     7681 atcggcaagt ggcacctcta ctcggcgtat ggcgtgagcg gcggcatgag cctgtcgcag
     7741 gcgagccgca ccccgattcc ggcgagccac cggcgcggct tcgaccactg gcagggcttc
     7801 gagctccgga acgacttcgc ggacacctgg atcttcgagg gcgacgaccc cctgccccgc
     7861 catttgccgg gccatcagac cgatgccctc ttcgacctgg cgctggacta cctcgagcgc
     7921 aagcgacccg cgtcgcaacc cttcttcctg atgctctcga tcgaggcgcc gcacccgccc
     7981 ttcatggcca cggccgagac cctcgcccgc gtccaggccc gcggccccct gcagcaccgc
     8041 atcaacgtgg acctcggcgc catccgcttc tttccgccgg aatggcacga ggcgaccggc
     8101 ccggcgggga ggatcgaccc ggcagacccg gcctcggtga agcgcgtctt cgaggccaac
     8161 atgcaggcct attacgccat gatcgagcag atcgacgaca atatgggccg gctccgggcc
     8221 accctcgagc ggcagggcct cgccgacgac acgatcaccg tcttcctcgc cgaccacggc
     8281 gagctcggcg gcagccacgg gcttctcggc aaggcggaac cctgggagga atcgatcggc
     8341 gtgccgctga tcgtggccgg gccgggggtg ccgcccgggc gcgaggccgc catgccggtg
     8401 cataccgagg atcttttttc gacctttgtc ggcctcgccg gtggatcgga gcgtcccgcg
     8461 cccccccggg tcgacctcgc ccccttcatc cgtggggagg cgccgccgcc ggagcgaccg
     8521 ggcgtgatgc tcgagttcgt caccgagacc cgcgccaacc ggagctacta cgacaagcgc
     8581 tggcgcggga tccgcggccc gcgtcacaag tacacggtcc tgggcgacat gacaggcgcc
     8641 ctgccgtggc agctcttcga cctcgaggcg gacccgttcg agctcaggaa cctgctggcg
     8701 gagccggggc acgagccggt ggcggcagcg atgcaccgcg ccctgatcgg gcttttggac
     8761 gccgccgccg acgatttcgc cctggccccg gccttcggct cgccagcccg tcacgccgtg
     8821 cccgagcgcg tttcctgacg ctggttcagc ttgcgcaatt gctcgaccag cttcgcatag
     8881 tcgacggcgc gcgagtattc gaggatgtgc acctccatca ggcggtcgaa ggtcgcctgg
     8941 tcgccggtgc ggagcgccgc gatcacctcg tcatgctcgg cgacgatgct cttcaggctc
     9001 ttcttcaggg tcgagaggcc gaagatgatg gtgagctggc gcgacagcga ttcccagcag
     9061 cgcagcagcg tgtcgttgcc ggagagccgg cagagcagca tgtggaactc ggtatcgagg
     9121 ctcgccacga ggaacttgtc gtccgcgtcc gccgccctgt gcatcctggc cagcatctcg
     9181 tcgagcggcg cgaggaagcc cttctgcccc tggcgcagcg ccatgatttc ggccccggcg
     9241 agcttctcca gcgccaggcg gaccttcagg atcttctcga gccggtcgat gtcgacgctc
     9301 atcagccgca tgccgcgata gcgctcgctg accacgacac cctggctctc gagcaggcgc
     9361 agcgcctcgc gcaccggaat ccggctcacc tccagggcgc gcgccacctc ggcctcgacg
     9421 attcggtcgc cgggcaggaa gacgccgcgc gccgaggcct cgacgatggc gtcaaccacc
     9481 tggttgacga gcgtcttcgc tgcgagcggc gccagcccgc tcaggcccgg cttggctgga
     9541 gttgcgctgg cagtcggcat cgccggctgg ttcctcgaca cgcttggttt gacaagtatc
     9601 gtatacgatt atacgattct gcttaagagg ttttccatag ccttcattcg acttcgcgac
     9661 aagccagggg tgatccagca tcacccgcca gaggataggg agcacgtgaa acgatgaagc
     9721 ggcacacgaa cctaaaatct ttcgcccgga cgctggcgat ggccggaacc gtcgccctga
     9781 cctccgcgct catggcgacg agcgcgctgg cccaggtcat gggcggcgac gtcgtcgtcg
     9841 tccagggctc caacccgccg agcctcgacg ccatgtcgac ctcgtcgcag gcctcgcgca
     9901 acgtcaacat gaacatgtac gagacgctct acggcttctc ggacgccatc caaccgattc
     9961 cgatcctcgc cgagggcgtg gagatctcgg acgacgggct gacctacgtc ttcaccctcc
    10021 gtcagggcgt gaagttccac aatggcgacg agatgaaggc ctcggacgtc aaggcctcgc
    10081 tcgagcgcta ccgcaaggtc ggcgccaccg gcaacctcct ggagccggtc acgaacatcg
    10141 aggtcaccgg cgactacgag gtgaccttca ccatggccaa ggcgacgccg accttcctcg
    10201 aggcgttctc gtcgccccgg gccccggcgg tcatcatccc cgaggaggat gccgaggccg
    10261 aggccggcaa gaccagcctg atcggcac
//
LOCUS       DAOTFI010000179        10241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7765_length_10241_cov_6.404575, whole genome shotgun sequence.
ACCESSION   DAOTFI010000179 DAOTFI010000000
VERSION     DAOTFI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7765_length_10241_cov_6.404575"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1081)
                     /locus_tag="P5558_16835"
     CDS             complement(89..1081)
                     /locus_tag="P5558_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26036.1"
                     /translation="MSDRRVEGGPVLAIESLAVAFPRYGGEPVHVLRDIDLVMAPGEV
                     VGVVGESGSGKTMLARSIMRLVPVPGRIDRGRVVFAGRDMAELDAETIRRMRGRDIAM
                     IIANPRGELDPLETVGQQIGNVLRHHLTLDRAAIRVRTLELLRQVSIPDPERRLDAYP
                     HELSGGMAQRVVMAIALACQPRFIISDDATSGLDVTVQAQVLELMRKLVVERGTSLLF
                     ITRDIGITAHFCDRVAVLFAGEIVEIAPRHAFFADPRHPYAILLLAAYAQNERLRRYW
                     LGDTTAGAAEGPATTGCPFARRCVRAQDRCRAEHPRLEEMAPGHFARCHFPVER"
     gene            complement(1078..2085)
                     /locus_tag="P5558_16840"
     CDS             complement(1078..2085)
                     /locus_tag="P5558_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26037.1"
                     /translation="MQLLAYIGRRLLFVAPQLVGILLVSFLLLKLIPGDPAVMMLGPF
                     ATDDGLARLRQELGLDRTILEQFLIYLGNVVQGDLGTSWQTTLPVTTDLAQRFPATLE
                     LVTLALLLALAIGVPLGIAGARYKRGVLRTTADYYGLFAGALPDFWFGLVLIFVFYTL
                     LQVVPPPLGRLDYAILPPATVTGSLLIDSALAGNWQALRNAAGHLILPVATLGLINAG
                     PILKMTQSTMERMLESDFSRYAEMCGLPDRVVARKALRNALPSIVTIVSVLYGFLIGG
                     AVLVEIVFSWGGAGQYAVGGVLNADLNPVLGFVLYSAILSLVIYLLVDLIYFAIDPRT
                     R"
     gene            complement(2085..3005)
                     /locus_tag="P5558_16845"
     CDS             complement(2085..3005)
                     /locus_tag="P5558_16845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26038.1"
                     /translation="MAFFLALLRARPSYALGYIIVGVVVLLAVLAPWVAPFDPVRASP
                     ADFLKPPGWPYLMGTDATGMDIFSRVIHAPRIDLAIALAGTLISAVVGSALGAAAGYY
                     QTQRGVGAFASGFVMRAADVLQAFPVFVFAIAVVAVLGQSLQSIVIAVAFVNTPIYLR
                     LMRSQVLAIRRMRYVEAAYIAGVSDLQILTRHVVTNAMAPILAQLSVNIGWAILLTAA
                     LSFIGAGVEAPTPEWGSMIAMGYQSIITGHWWPSIFPGLALALTVFGFALIGSSVEVL
                     ADPARRRALAGELEARRRLRLQARAAGAGA"
     gene            complement(3100..4752)
                     /locus_tag="P5558_16850"
     CDS             complement(3100..4752)
                     /locus_tag="P5558_16850"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26039.1"
                     /translation="MSDPRLPFFDRRTFFRTTTAIAGAAVMPRILAISPAMAQAGSTM
                     VIAAPATPQSLDCNFDVSLGTFEAIGALYDGLLGFEKIPDPGVPDARREDISFHPELP
                     GGVNMVGKLAESWELDPSGTRVVFKLREGVMSNWGNELTAEDVRWTWNRAFELGALGA
                     FYTAVSGLTSPDDVRVEDKYVVSFNTQSPNPLLLKIHTNLYTPVYDSTKCKEVGGTDD
                     PWARKFIENDSAGFGPYRLQQLTRGQQAVFAARDDYYGGKPAIDTVVFREVPTSAARV
                     QLLQGGAVDIAQYLQPLEIISLQRVPNVVVETVPASFMIWIELNAKMAPFDNVDVRRA
                     MNFAFPQQQVLETVYQGLASPLDGCMPNIYPGFAGEFFEYEYDPAKAKELLAGAGFAD
                     GFTTSLSYNAGDPVQEPIAIIYQSALREIGVELELKKVPAGSFYNMVTERGQPMIFYV
                     DSPWCPDPGYSLTLYFDSKSYVNYSNYANAEVDRLIAETAATADEAARLDMMRQAQEI
                     IMREAPWVFVAYPNYTMARKADIEGWTYYTSNNIRFQDFNRA"
     gene            4960..5703
                     /locus_tag="P5558_16855"
     CDS             4960..5703
                     /locus_tag="P5558_16855"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY26040.1"
                     /translation="MDSSDQYSAPMADPAATAPVTQRALTRIQAMLRSGAFPPGSSLP
                     PQRRLADELRVSRASLREALSILGTLGIIRVEHGRGTFVAARGDDDPGSEPVPAARRF
                     KGRYSEAEVYQFRCIVEPRAAALAALRLTLGEIGRLEATNRAFARATRELDLVASAEA
                     DWEFHDLLMQFSHNRLLVDIYHGYRPVMIESQRLPLARRSRLLEPIGEHEHLLRALGM
                     HDPDGASYYMRMHIGRAADRIGLSFVDVG"
     gene            5736..>5980
                     /locus_tag="P5558_16860"
     CDS             5736..>5980
                     /locus_tag="P5558_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26041.1"
                     /translation="MTAIERVAAGSAAAIGRAIAAGEVDPVALTEHLLERIGASAEPV
                     FITVTAARARAEAEAARARQVAGRPLSALDGVAVAWK"
     assembly_gap    5981..6080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6081..6986
                     /locus_tag="P5558_16865"
     CDS             <6081..6986
                     /locus_tag="P5558_16865"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRY26042.1"
                     /translation="GGSSSGSAVAVAAGLAPVAIGTDTGGSVRIPAAFNGLVGYKSSG
                     GRIDKRGVFPLSDTLDSIGPIGRTVEDCILVEMLLRGAATGAVRRAPLAGQRFVLATN
                     VVLDEIEADVATALEAAVDDLAKSGAQIESRPVPVLDEVMRVTAERGTLTAAEAYTVH
                     KALVEGPEVERIDRRVVSRILNGKRMSAADVLIIQRERRRLMAELAADLGGAVLVMPT
                     TVHVAPPIAPLEADVALFHSTNLRTLRNTMLGNFLDLCGLAIPSGITPSGMPASILFS
                     LPAGEDDRLLSLGLELDRVLLPRRL"
     gene            7036..7998
                     /locus_tag="P5558_16870"
     CDS             7036..7998
                     /locus_tag="P5558_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HRY26043.1"
                     /translation="MSRKRVVAAAQLGPIARSESREQVVKRLLALLAEARGKGVGFIV
                     FPELALTTFFPRWMMEDEAELDSFYEGKLPGPAAEPLFDAAKAAGIGFYLGYAERFVE
                     AGRVRRFNAAITVAPTGEIVGKYRKVHLPGHAEPQPNRAFQHLEKRYFEPGDLGFPVF
                     EALGGRVGMLICNDRRWPEGYRVMGLQGVELVCLGYNTPLDHANHETINVLSGFHNAL
                     SMQAGAYQNASWVIGTAKCGREEGSLMLADSMIIAPSGEIVARATTMEDELVAATIDL
                     DMGLPYKETFFDFARHREPTSYGLIIERKGAGDMLPPARGAGID"
     gene            8036..>10241
                     /locus_tag="P5558_16875"
     CDS             8036..>10241
                     /locus_tag="P5558_16875"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY26044.1"
                     /translation="MPLERNRLVTMDQALLAIALLGLVGGGLAWWLADPAIARWFWRV
                     GTGIVLAALVVEIGRNLLHGETGVDILAALSMTGALLLDEPLAGNIVALMFSGGTMLE
                     AVADRRARRELTHLIGRTPRTAHLDTGHGLQDVAIEAIRPGDRLLVKTGEVLPVDGML
                     EAAPALLDESALTGESLPVERQVGDTLASGTLNLGAPLRLRATATAEASTYAGIVRLV
                     EAAQREKAPFVRLANRYSMIFLPASLALAGLAWLLSGDPTRALAVLVVATPCPLILAA
                     PVAIMAGMSAAAKRGILVKGGGALETLARVSTLVFDKTGTLTTGRARIATIDRYGTVG
                     ESELLRLAASLDQFSTHPIAATLRRAAERQGLGLAMADQIEEVGGTGIQGRVEGRDVR
                     LGSLAFVAEESDADEAGDLAAGTLRHAARAGAAIVFISVDGRLAGAFVVTDEIRPETP
                     RTLRDLHRAGIHRVVMLSGDRHDVAEAVAAAVGVDTVLADRSAEDKVDAVRAERAEAV
                     TVMVGDGINDAPALAAADVGVAMGARGAGAASEAADIVLLVDRLDRLTEGIGIARHTR
                     RIALESVIAGMALSTAGMVVAAFGYLPPVAGALVQEVIDVAVILNALRAIGGGLPRAV
                     KGELEPTDLRRLKAEHAAFAPALERMARLAERLDASGDGTLMAELRAVDTVLQEKILP
                     HERADERELHPGLAEILGGRDPMAAISRTHSEIGHLIRLFHRLVAGLPADGPD"
BASE COUNT         1577 a         3453 c         3661 g         1450 t
ORIGIN      
        1 acgatggccc cgcatcgcgg cgggccggtc tcctcgccca aagccagggc tggcgtgtgc
       61 aggtcatggc catttcccgg acaggcacct agcgctccac cggaaaatgg cagcgggcga
      121 aatggccggg ggccatctct tcgagccgcg gatgctcggc gcggcagcgg tcttgcgccc
      181 gcacgcagcg ccgggcgaaa gggcagccgg tcgtggccgg cccctcggcc gcacccgccg
      241 tcgtgtcgcc gagccagtag cggcgcaggc gctcgttctg ggcataggcc gcgagcagca
      301 ggatggcata aggatgcctg gggtcggcga agaaggcgtg gcgcggggcg atctcgacga
      361 tctcgccggc gaagagcacg gcgacgcggt cgcagaaatg cgcggtgatg ccgatgtcgc
      421 gggtgatgaa gaggagcgag gtgccgcgct cgacgacgag cttgcgcatc agctcgagga
      481 cctgggcctg aaccgtgacg tcgaggccgc tggtggcgtc gtcggagatg atgaagcggg
      541 gctggcaggc gagcgcgatg gccatcacga cgcgctgcgc catgccgccc gacagctcgt
      601 gcgggtaggc gtcgaggcgg cgctccgggt cggggatgct gacctggcgc aggagctcca
      661 gcgtgcgcac gcggatggcc gccctgtcga gcgtcaggtg gtggcggagc acattgccga
      721 tctgctggcc taccgtctcc agcgggtcga gctcgccgcg cgggttggcg atgatcatgg
      781 cgatgtcgcg gccacgcatc cgccggatcg tctcggcgtc gagctcggcc atgtcgcgcc
      841 cggcgaacac caccctgccc cggtcgatgc ggccgggcac cggcacgagc cgcatgatgg
      901 agcgggcgag catggtcttg ccggagcccg attcgccgac cacgcccacg acctcgcccg
      961 gcgccatgac gaggtcgatg tcgcgcagca catggacggg ctcgccgcca tagcgcggaa
     1021 aggccacggc gaggctctcg atggcgagga ccggaccgcc ctcgacccgt cggtcgctca
     1081 tcgcgtcctc gggtcgatgg cgaagtagat caggtcgacg agcaggtaga tgaccagcga
     1141 gaggatcgcc gagtagagca cgaagcccag gaccgggttg aggtcggcat tgagcacgcc
     1201 acccaccgca tactggccgg cgccgcccca ggaaaagacg atctcgacca gcacggcacc
     1261 gccgatgagg aagccgtaga gcaccgagac gatggtgacg atcgacggca gcgcattgcg
     1321 cagcgccttt cgcgcgacca cgcggtcggg caggccgcac atctcggcat agcggctgaa
     1381 atcgctctcc agcatgcgct ccatggtgga ttgcgtcatc ttgaggatcg gcccggcatt
     1441 gatcaggccg agcgtcgcca cgggcaggat caggtggccc gcggcattgc gcagcgcctg
     1501 ccagttgccg gcgagcgcgc tgtcgatgag cagcgagccg gtgaccgtgg cgggaggcag
     1561 gatggcgtaa tcgaggcggc cgagcggcgg cggcacgacc tgcagcaggg tgtagaagac
     1621 gaagatgagg acgaggccga accagaagtc cggcagggct ccggcgaaga gcccgtaata
     1681 gtcggccgtc gtgcgcagca cgccgcgctt gtagcgcgcc ccggcgatgc cgagcggcac
     1741 gccgatcgcc agcgccagca ggagcgccag cgtcacgagc tcgagggtcg ccgggaagcg
     1801 ctgggcgaga tcggtggtga ccggcagcgt ggtctgccat gaggtgccga gatcgccctg
     1861 cacgacattg ccgagataga tcaggaactg ctcgaggatc gtgcggtcga ggccgagctc
     1921 ctggcgcagc cgggcgaggc cgtcgtcggt ggcgaagggg ccgagcatca tcaccgccgg
     1981 atcgcccggg atcagcttca ggagcaggaa gctgacgagc aggatgccga ccagttgcgg
     2041 tgcgacgaag agcaggcggc ggccgatata ggcgaggagc tgcatcaggc ccctgccccg
     2101 gccgcacggg cctgcagccg aaggcgacgg cgcgcctcga gctcaccggc gagcgcccgc
     2161 cgccgggcgg gatcggccag cacctcgacg ctggagccga tgagggcgaa gccgaagacg
     2221 gtgagcgcca gagcgaggcc cgggaagatc gacggccacc agtggccggt gatgatgctc
     2281 tggtagccca tggcgatcat gctgccccat tccggggtcg gcgcctcgac gccggcgccg
     2341 atgaagctga gcgccgcggt cagcaggatg gcccagccga tattgaccga gagctgggcc
     2401 aggatcggcg ccatggcgtt ggtgaccaca tgccgggtga ggatctgcag gtcggagacg
     2461 ccggcgatat aggccgcctc gacatagcgc atgcggcgga tggccagcac ctggctgcgc
     2521 atcagccgga gatagatcgg ggtgttcacg aaggccacgg cgatgacgat gctctgcagg
     2581 ctctggccga gcaccgccac gaccgcgatg gcgaagacga agaccggaaa ggcctgcagc
     2641 acgtcggcgg cgcgcatgac gaagccggag gcgaaggcgc cgacgccacg ctgggtctgg
     2701 tagtagccgg cggccgcccc cagggcggag ccgaccacgg ccgagatcag cgtgccggcg
     2761 agggcgatgg cgaggtcgat ccggggcgcg tggatgaccc ggctgaagat gtccatgccg
     2821 gtggcatccg tgcccatcag atagggccag ccgggcggct tcaggaagtc ggccgggctg
     2881 gcgcgcaccg gatcgaaggg cgccacccag ggggcgagga cggcgaggag aacgaccacg
     2941 ccgacgatga tatagccgag cgcataggac ggccgggccc gcagcagagc gaggaagaag
     3001 gccaagcgtc ccgagccgcc ggcggctgcc ggtggctcgg gctcggcaat cctcgtggct
     3061 gacgtccgct gcgccacgtt gtctgccctg atccgccgct caggcgcggt tgaaatcctg
     3121 gaagcggatg ttgttggagg tgtagtaggt ccacccctcg atgtcggcct tccgcgccat
     3181 ggtgtagttg ggatatgcca cgaagaccca gggcgcctcg cgcatgatga tctcctgcgc
     3241 ctggcgcatc atgtcgagcc gtgccgcctc gtcggccgtg gcggcggtct cggcgatcag
     3301 ccggtcgacc tcggcattcg cgtagttgct gtagttcaca tagctcttgc tgtcgaagta
     3361 gagcgtcagc gaatagccgg gatcggggca ccacgggctg tcgacataga agatcatcgg
     3421 ctggccgcgc tcggtgacca tgttgtagaa ggagccggcc ggcaccttct tcagctcgag
     3481 ctcgacgccg atctcgcgga gcgccgactg gtagatgatg gcgatcggct cctgcaccgg
     3541 atcgcccgcg ttgtaggaga gcgaggtggt gaagccatcg gcgaagccgg cgccggcgag
     3601 cagctccttg gccttggccg ggtcgtactc gtactcgaag aattcaccgg cgaagcccgg
     3661 atagatgttg ggcatgcagc cgtcgagcgg gctcgccagg ccctgataga ccgtctccag
     3721 cacctgctgc tgcgggaagg cgaagttcat cgcccggcgc acgtcgacat tgtcgaaggg
     3781 cgccatcttg gcgttgagct cgatccagat catgaacgac gccggaaccg tttcgaccac
     3841 gacgttcggc acgcgctgca ggctgatgat ctcgagcggc tgcagatact gggcgatgtc
     3901 cacggcacca ccctggagca gctggacccg cgccgccgag gtcggcacct cgcggaagac
     3961 cacggtatcg atggcgggct tgccgccata gtagtcgtcg cgggcggcga agacggcctg
     4021 ctggccgcgg gtgagctgct gcagccggta ggggccgaag ccggcgctgt cgttctcgat
     4081 gaacttgcgc gcccatggat cgtcggtgcc gccgacctcc ttgcacttcg tgctgtcgta
     4141 gaccggggtg tagaggttgg tgtggatctt caagagcaac gggttcggcg actgggtgtt
     4201 gaacgagacc acgtacttgt cctcgacccg gacatcgtcc gggctcgtga ggccggaaac
     4261 cgccgtgtag aaggcgccga gcgcgcccag ctcgaaggca cggttccagg tccagcgcac
     4321 gtcctcggcg gtcagctcgt tgccccagtt gctcatcacc ccttcgcgca gcttgaagac
     4381 gacgcgcgtg ccggagggat cgagctccca gctctcggcg agcttgccca ccatgttgac
     4441 gccgcccggc agctcgggat ggaacgagat gtcctcgcgc cgcgcgtccg gcacgccggg
     4501 atcggggatc ttctcgaagc cgagcaggcc gtcatagagg gcgccgatcg cctcgaacgt
     4561 gccgaggctg acgtcgaaat tgcagtccag gctctgcggc gtcgccgggg cggcgatcac
     4621 catggtcgac ccggcctgcg ccatggccgg cgagatggcg aggatgcgcg gcatgacggc
     4681 agcgccggcg atggccgtgg tggtgcggaa gaaggtgcga cgatcgaaga acggcagccg
     4741 agggtccgac atgtggcctc ctgttgtctg gacggtggcg ggaccgattg ttcgttgggt
     4801 ggtctgacca cccgaggcgt catcgacgaa tcatcatcga cgacctgtcg cccgcgtcaa
     4861 gcggcaagtt ccttgccatg ggaacggatc agggcaattc tggcgaccct tgagaagcag
     4921 ccggcccagg ccgacgattg atgcgctggt cggaacaata tggactcatc agaccaatat
     4981 tcggcaccaa tggcggaccc ggcggcgacg gcgccggtca cgcagcgtgc cctgacccgc
     5041 atccaggcga tgctgcgctc cggcgccttc ccgccgggct ccagcctgcc gccgcagcgc
     5101 cggctggcgg acgagcttcg ggtcagccgc gcgtcgctgc gcgaggcgct ctcgatcctc
     5161 ggcacgctcg gcatcatccg ggtcgagcac ggccgcggca ccttcgtcgc ggccaggggc
     5221 gacgacgacc cgggcagcga gccggtgccg gccgcccggc gcttcaaggg gcgctactcg
     5281 gaggcggagg tctaccagtt ccgctgcatc gtcgagccgc gcgcggcggc ccttgcggcg
     5341 ctgcgcctga cgctcgggga gatcggccgg ctggaggcca ccaaccgggc cttcgcccgg
     5401 gcgacccgcg agctcgacct cgtcgccagc gccgaggccg actgggaatt ccacgacctg
     5461 ctcatgcagt tctcgcacaa ccggctgctg gtcgatatct atcacggcta ccggccggtc
     5521 atgatcgaga gccagcggct gccgctggcc aggcgcagcc ggctgctgga gccgatcggc
     5581 gagcacgagc acctgctcag ggcgctcggg atgcacgacc cggacggcgc cagctactac
     5641 atgcggatgc atatcggccg ggccgccgac cgtatcggcc tgagcttcgt cgacgtcggc
     5701 tgaccagtgc cggggcgcag gggaggaacg agggaatgac agcgatcgag cgggtggcgg
     5761 cgggttcggc ggcggccatc gggcgggcca tcgcggccgg cgaggtcgac ccggtggcgt
     5821 tgaccgagca tctgctggaa cgcatcggtg cttcggccga gccggtcttc atcaccgtca
     5881 ccgccgcccg cgcccgggcc gaggccgagg cggcgcgcgc ccgccaggtc gccggccggc
     5941 cgctctcggc gctcgacggc gtggccgttg cctggaagga nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn ggcggctcgt cctcgggctc ggccgtcgcc gtggccgccg
     6121 gcctggcacc tgtcgccatc ggcaccgaca cgggcggctc cgtgcgcatc ccggccgcct
     6181 tcaacggcct cgtcggctac aagagctcgg gcggccggat cgacaagcgc ggcgtctttc
     6241 ccctctcgga cacgctcgac agcatcggcc ccatcggccg cacggtcgag gactgcatcc
     6301 tcgtcgagat gctgctccgc ggggccgcga ccggcgccgt gcggcgcgcg ccactcgccg
     6361 gccagcgctt cgtcctcgcg accaacgtcg tgctcgacga gatcgaggcc gacgtcgcca
     6421 ccgcgctgga agcggcggtc gatgacctgg cgaaatccgg agcgcaaatc gagtcgcggc
     6481 cggtgccggt gcttgacgag gtgatgcggg tcaccgccga gcgcggcacg ctgaccgcgg
     6541 ccgaggccta caccgtccac aaggcgctgg tcgaaggccc cgaagtcgag cgcatcgacc
     6601 ggcgcgtggt gtcgcgcatc ctgaacggca agcggatgtc ggccgccgac gtgctgatca
     6661 tccagcgtga gcgccggcgg ctcatggccg agctcgcggc cgatctgggc ggtgccgtcc
     6721 tggtcatgcc gacgaccgtc cacgtggccc cgcccatcgc cccgctcgag gccgacgtgg
     6781 ccctcttcca cagcaccaac ctgcgcacgc tccgcaacac catgctcggc aactttctcg
     6841 atctctgcgg cctcgccatt ccgagcggca tcaccccctc gggcatgccg gccagcatcc
     6901 tcttcagcct gccggcgggc gaggacgacc ggctcctctc gctcggcctc gagctcgacc
     6961 gcgtgctcct gccgaggcga ctttgatgaa gcgttttggg ttcacgttcg acgatcaagg
     7021 ggaagggaca cggtcatgag caggaagcgg gtcgtcgccg ccgcccagtt gggcccgatc
     7081 gccaggtccg agagccgaga gcaggtggtc aagcgtctcc tggccctgct cgccgaggcc
     7141 agaggcaagg gcgtcgggtt catcgtcttc ccggagctgg cgctcaccac cttcttcccg
     7201 cgctggatga tggaggacga ggccgagctc gacagcttct acgagggcaa gctgccgggg
     7261 ccggcagcgg agccgctctt cgacgcggcg aaggcagcgg gcatcggctt ctatctgggc
     7321 tatgccgagc gcttcgtcga agccggccgc gtgcgccgct tcaatgcggc gatcacggtg
     7381 gcaccaaccg gcgagatcgt cggcaagtac cgcaaggtcc acctgccggg ccacgccgag
     7441 ccgcagccga accgcgcctt ccagcatctc gagaagcgct atttcgagcc gggtgacctg
     7501 ggctttccgg tgttcgaggc gctggggggg cgggtcggca tgctgatctg caacgaccgg
     7561 cgctggcccg aaggctaccg ggtcatgggc ctgcagggcg tcgagctggt ctgcctcggc
     7621 tacaacacgc cgctcgacca cgccaatcac gaaacgatca acgtgctctc cggcttccac
     7681 aacgcgctgt cgatgcaggc cggcgcctac cagaacgcga gctgggtgat cggcaccgcc
     7741 aaatgcggcc gcgaggaagg ctcgctgatg ctggccgaca gcatgatcat cgccccctcg
     7801 ggcgagatcg tcgccagggc caccaccatg gaggacgagc tggtggccgc caccatcgat
     7861 ctcgacatgg gcctgcccta caaggagacc ttcttcgact tcgcccgcca ccgcgagccc
     7921 acgagctacg gcctcatcat cgagcgcaag ggagcgggcg acatgttgcc accggcacgg
     7981 ggtgccggaa tcgactaggc tggccgaagt ccccttctgg ccgttggtcc gacacatgcc
     8041 cctcgaacgc aaccgtctcg tcaccatgga ccaggcgctc ctcgccatcg ccctcctcgg
     8101 gctggtcggc ggcgggctcg cctggtggct ggcggacccg gccatcgccc gctggttctg
     8161 gcgcgtcggc acgggcatcg tgctcgccgc cctggtggtc gagatcggcc gcaatctgct
     8221 gcacggcgag accggcgtcg acatcctggc cgccctctcg atgacgggtg cgctgctgct
     8281 cgacgagccg ctggccggca atatcgtggc cctgatgttc agcggcggca cgatgctcga
     8341 ggccgtggcc gaccgccggg cgcggcgcga gctcacccac ctgatcggcc gcacaccccg
     8401 cacggcgcat ctggacaccg gccatgggct ccaggacgtg gcgatcgagg cgatccgacc
     8461 gggcgatcgg ctcctggtca agaccggcga ggtcctgccg gtcgacggca tgctcgaggc
     8521 ggcacccgct ttgctcgacg aatcggcgct caccggcgaa tcgctgccgg tcgagcggca
     8581 ggtcggcgat acgctcgcga gcggcacgct caacctcggc gcccccttgc gcctgcgcgc
     8641 cacggccacg gccgaggcca gcacctatgc cgggatcgtc cgcctggtcg aggcggcgca
     8701 gcgcgagaag gcacccttcg tgcgcctcgc caatcgctac tcgatgatct tcctgccggc
     8761 gagcctggcc ctcgccggcc tcgcctggct cctctccggc gacccgacgc gagcgcttgc
     8821 cgtgctcgtg gtcgcgaccc cctgcccgct gatcctggcg gcacccgtgg cgatcatggc
     8881 cggcatgtcg gcggcggcga agcgcggcat cctggtcaag ggcggcgggg cgctcgagac
     8941 gctggcccgc gtctccaccc tggtcttcga caagaccggc accctgacca cgggccgtgc
     9001 ccggatcgcc acgatcgatc gctatggcac ggtcggcgag agcgagctcc tgcgcctcgc
     9061 ggcctcgctc gaccagttct cgacccaccc gatcgccgcc acgctccgcc gtgccgcgga
     9121 gcgccagggg ctcggcctcg ccatggccga ccagatcgag gaggtgggcg gcaccggcat
     9181 ccagggtcgg gtcgagggcc gggacgtgcg cctcggcagc ctcgccttcg tcgccgagga
     9241 aagcgacgcc gacgaggccg gggacctggc ggcgggcacc cttcggcacg cagccagggc
     9301 cggtgcggcg atcgtcttca tcagcgtcga tggcaggctc gccggcgcct tcgtggtcac
     9361 cgacgagatc cgccccgaga ccccgcgcac gctgcgcgac ctgcatcggg ccggcatcca
     9421 ccgcgtcgtc atgctgagcg gcgaccggca cgacgtggcc gaggcggtgg ccgccgccgt
     9481 cggcgtcgac accgtgctcg ccgaccgctc ggccgaggac aaggtggacg cggtcagggc
     9541 ggagcgggcc gaggcggtga cggtgatggt cggcgacggg atcaacgacg ccccggccct
     9601 ggccgccgcc gatgtcggcg tcgccatggg ggcgcgcggt gccggtgcgg cgagcgaggc
     9661 ggccgacatc gtgctgctcg tcgatcggct ggaccggctg accgaaggga tcggcatcgc
     9721 ccgccacacg cggcgcatcg ccctcgagag cgtgatcgcc ggcatggcgc tgtcgacggc
     9781 cggcatggtc gtggccgcct tcggctatct gccaccggtg gcgggggcgc tggtgcagga
     9841 ggtgatcgac gtggcggtca tcctgaacgc gcttcgggcg atcggcggcg gcctgccgcg
     9901 cgcggtgaag ggcgagctcg agccaaccga tctgcgccgg ctcaaggccg agcacgccgc
     9961 ctttgcaccc gctctggagc ggatggcacg gctcgccgag cgactcgatg cgtccgggga
    10021 cggcacgctc atggcggagc ttcgcgccgt cgacaccgtg ctccaggaga agatcctgcc
    10081 gcacgagcgg gccgacgagc gcgagctcca tccgggcctc gccgagatcc tcggcggtcg
    10141 cgaccccatg gccgcgatca gccgcaccca tagcgagatc ggccacctga tccgcctctt
    10201 tcatcggctc gtggcgggcc tgccggcgga cgggccggac g
//
LOCUS       DAOTFI010000180        10215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7790_length_10215_cov_7.068898, whole genome shotgun sequence.
ACCESSION   DAOTFI010000180 DAOTFI010000000
VERSION     DAOTFI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7790_length_10215_cov_7.068898"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2019
                     /locus_tag="P5558_16880"
     CDS             <1..2019
                     /locus_tag="P5558_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HRY26045.1"
                     /translation="PQTGWLPLAFALDWGRPPRQQNSTSFWYAHTDQWRYETVTADEI
                     LSPTAPAGDWQASLIDFNVRAERMGWLPSAPQLKENPLGIAAKAAAAGLEPKDYVAKS
                     LKDGTLELSCHDPDDPANWPRNLFVWRSNLLGSSGKGHEYFLKHLLGTSNGVMGKNLG
                     EEGGVKPKDVKWHDEAPEGKLDLLVTLDFRMSTTCAFSDIVLPVATWYEKNDLNTSDM
                     HPFIHPLMAATDPAWECKSDWEIYKAIARQFSAIAPEVLGVEKDVVLVPIQHDSPGEL
                     AQAYDVKDWKKGEIEPIPGRTMPTVAVVERDYPNVYKRFTALGPLMEKVGNGGKGIAW
                     QTGHEVDLLRALNGAVTEDGPTKGMARIDSDIDATEVVLMLAPETNGEVAVKAWEALS
                     KITGREHAHLALTKEDEKIRFRDIAAQPRKIISSPTWSGLESEKVCYNAGYTNVHELI
                     PWRTLSGRQQLYQDHDWMRAFGEAFVTWKPPIDPKSVARIQGKLPNGSTEIVLNFITP
                     HQKWGIHSTYTDNLLMLTLSRGGPIVWLSEDDAKKAGIVDNDWVEVYNTNGALTARAV
                     VSQRVNPGMMLMYHAQEKMVNTPGAETTKKRGGIHNSVTRAILKPTHMIGGYAQLSYG
                     FNYYGTVGSNRDEFVIVRKMQNVDWLEGPLNRPALEETSQPVGVEAAE"
     gene            2016..3545
                     /gene="narH"
                     /locus_tag="P5558_16885"
     CDS             2016..3545
                     /gene="narH"
                     /locus_tag="P5558_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HRY26046.1"
                     /translation="MKIRAQIAMVLNLDKCIGCHTCSVTCKQVWTNREGMEYAWFNNV
                     ETKPGIGYPKEWENQKKWNGGWVRKSNGKIEPRIGGKWRVLAKIFANPDLPEIDDYYE
                     PFTFDYDHLQSAPELEATPTARPRSLITGERMEKIEWGPNWEEILGGEFAKRSKDVNF
                     EGVQKEIYGAFENTFMMYLPRLCEHCLNPTCVAACPSGAIYKREEDGIVLIDQERCRG
                     WRMCVSGCPYKKIYYNWASGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVVLYDA
                     DGIEAAAATTNEQDLYEAQLELFKDPNDPEVIAQARADGVPEAWLEGARRSPIWKMAM
                     DWKIAFPLHPEYRTMPMVWYVPPLSPIQAAAEAGQMGVDGDMPDVRSLRIPLKYLANL
                     LTAGKEEPIALGLERMLAMRAYMRAKTVDGVIDEAIAERVGMKGLDIEEMYELMAIAN
                     YEDRFVIPTSHRETGVDPYELRGSCGFTFGNSCSGGTSDATLFFGAPSRKKPKTPMEV
                     S"
     gene            3545..4258
                     /gene="narJ"
                     /locus_tag="P5558_16890"
     CDS             3545..4258
                     /gene="narJ"
                     /locus_tag="P5558_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRY26047.1"
                     /translation="MASSGARSYKALSALLAYPTAELQGACGEIQAVLHQERLVPAHT
                     LPALDRLIAELASLDLYEIQERYTVLFDRSRSLSLHLFEHVHGESRDRGQAMVDLAAH
                     YERHGYWIRANELPDFLPLFLEFLSLLPRDEAHGLLQQPLPILEALEERLRRRGSTYI
                     AIFVALLAIAGSKAASVPLATTADDDPDDLEALDADWEEAAVTFGPGGAADADDACGK
                     SRLATRIRAEIRSVHDRPA"
     gene            4270..5028
                     /gene="narI"
                     /locus_tag="P5558_16895"
     CDS             4270..5028
                     /gene="narI"
                     /locus_tag="P5558_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HRY26048.1"
                     /translation="MATWYNTFFFGIYPYIALAVMIAGSIIRYDREPYSWRSGSSQML
                     RKKQLFWGSNLFHFGILFLFFGHLVGLLTPHWGYSWAFDASVKQLVAVIAGGIAGVVC
                     FVGLTLLLHRRLTDPRIRSTSTTMDIAILVILWVQLSLGLLTLPFSLSHSDGGTMMTL
                     AEWAQRILTFRGDAAILVAGVDWPYQVHIILGLTIFLLFPFSRLVHIWSVPVRYVWRP
                     GYQVVRSAGADWRVPGPVGRPRIEARAEPMEPAE"
     gene            5025..5870
                     /locus_tag="P5558_16900"
     CDS             5025..5870
                     /locus_tag="P5558_16900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY26049.1"
                     /translation="MSGMRIFDLESRPKLRQPPPVTVNGTVIDQAAIAREAQHHPAGS
                     AREAGEAAARALVIRHLLLEEAARLEIEGVPEIDDEGRRETPEEARISTLLRQEVQVP
                     EADEAAARRFYTQNQRRFTSPDLYEAAHILFSADAADPVAYGRAREQALAALARLAEQ
                     PQRFAAMARDVSACPSAASGGNLGQVTGDAVTPAFAEALRRAEPGRVHPEPVETPYGV
                     HVLRLERRIAGRVLPFEMVEERIREWLGARVWRHAVRQYVSILVGRAEIGGIELGGAQ
                     SPLVQ"
     gene            5883..6176
                     /locus_tag="P5558_16905"
     CDS             5883..6176
                     /locus_tag="P5558_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26050.1"
                     /translation="MMGELVGRLTEPGVAEATLIEAGELGLLARLSNAAGMLDADIGD
                     LTSHIVRTWLARADDEAWVQLIGVMNRSAVPGLAALVLMVEQALAELDTTTAH"
     gene            6197..6757
                     /locus_tag="P5558_16910"
     CDS             6197..6757
                     /locus_tag="P5558_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRY26051.1"
                     /translation="MPVDRPRESLDPLPAGLLRQPLDYLMADHHRQRVLCRLADALAA
                     TSGSDPDLARTVGAFISRDMAIHVIDEEQDLFPLLRRRASLEDAIDAVLGRLAAEHSS
                     GSALAARIGRALDEAALTGRPLDRSTRLDLAEFARSEREHLALENAVVMPLARLRLTA
                     GDLRALSLRMAARRGLLIDDPGPTAA"
     gene            6754..7356
                     /locus_tag="P5558_16915"
     CDS             6754..7356
                     /locus_tag="P5558_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRY26052.1"
                     /translation="MIDDLFRRNRAWSIARQERDPDFFPRLARQQRPAYLWIGCADSR
                     VPANEIVGLDPGELFVHRNVANLAPPSDINFLSVLSFAVAVLKVQHVIVCGHYGCGGV
                     RAALLDERNGIVDHWLEPVRGTYRRNAARIEAIGSEADRVNALCEFNVQDRVQDVAAT
                     TIIRDAWAAGQKLAIHGWIYGLADGLLRNLDVTVAGLEDS"
     gene            complement(7367..8053)
                     /locus_tag="P5558_16920"
     CDS             complement(7367..8053)
                     /locus_tag="P5558_16920"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HRY26053.1"
                     /translation="MTGWAEYGLAVLLFVVAHAVPPRTTLRARLTGALGERGYLVLYS
                     LLSLLLLYWLLVAAGRAPYVPLWDPAGWQAWIPNLVMPLALVLAVCGTAVANPFSLGG
                     SRRHEFDPARPGITAVSRHPLLLAVALWAIAHMVPNGDLAHALLFGGLAVLAVLGMIL
                     LDRRHRRRCGDRRWQALTRKTAFLPGGAPLPAPGDAFPAGRVALALLIWVALLLVHPA
                     LFGVSPLPPL"
     gene            complement(8050..8484)
                     /locus_tag="P5558_16925"
     CDS             complement(8050..8484)
                     /locus_tag="P5558_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY26054.1"
                     /translation="MRLTAFTDFGLRVLMRLAGSPGRVITTEALATELAVSRHHLTKV
                     VRALGAAGFVSTARGAGGGFRLARPAGAITVGDVVRQLEGRHALVECFRADGGACVLT
                     PGCRLKKHLASAREAFLTELETVTVADCAVAPPPAPRVAMAP"
     gene            8593..9816
                     /locus_tag="P5558_16930"
     CDS             8593..9816
                     /locus_tag="P5558_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRY26055.1"
                     /translation="MSATTAERIRTYRGPAVLGLGFRPFFLAAGLWSALAMLLWILLL
                     TGGIDLPIALAPVDWHVHSLLYGFVPATVTGFLLTAVPNWTGRLPVTGTPLLCLALLW
                     LAGRLAVLFGDVLGPLLSALVDLAFLAAVFAVILRELLAGRNKRNLPVLALVGLLFLG
                     NALFHLEAARTGGAYYGTRVGILAILMLIMLIGGRIVPSFTRNWLVKRGAGRLPASFG
                     RFDGVCLATAATALASWVVASEAAATAWLAGIAGALHLVRLGRWAGERTTAEPLLLVL
                     HVAYAFVPLGFFLVLLAHVLPSTVLPVAAIHAWLAGGVGLMTLAVMTRASLGHSGRAL
                     TADWPTVAIFCALVVAALARILAGTTLALPGLLHLAATAWILAFGGFAVAYWPVLTGV
                     AAGLKPSSPPPGRPR"
     gene            complement(9779..>10215)
                     /locus_tag="P5558_16935"
     CDS             complement(9779..>10215)
                     /locus_tag="P5558_16935"
                     /inference="COORDINATES: protein motif:HMM:NF027482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HRY26056.1"
                     /translation="PRASGDTAMRLLASSLLVLLLLTGHQAARAEGEPDAFRAVIEAQ
                     LEAFRHDDGGAAFSFASPTIRSLFGTPESFMAMVVQGYPQIYRPRSVTFGAVVTYRGQ
                     PAQMVHLVGPKGETVTAYYQMQQQPDGSWKINGVFLEAATTA"
BASE COUNT         1596 a         3657 c         3452 g         1510 t
ORIGIN      
        1 ccgcagaccg gctggctgcc gctcgccttc gcgctcgact ggggccggcc gccgcggcag
       61 cagaactcga cctcgttctg gtacgcccac accgaccagt ggcgctacga gacggtgacc
      121 gccgacgaga tcctctcgcc gaccgccccg gcgggcgact ggcaggcctc gctgatcgac
      181 ttcaacgtgc gcgccgaacg catgggctgg ctgccctccg cgccgcagct caaggagaac
      241 ccgctcggca tcgccgccaa ggcggcggcg gccgggctgg agccgaagga ctacgtcgcc
      301 aagtccctga aggacggcac gctcgagctc tcctgccacg acccggacga cccggcgaac
      361 tggccgcgca acctcttcgt ctggcgctcc aatctcctgg gctcctcggg caagggccac
      421 gagtacttcc tcaagcacct gctcggcacg tcgaacggcg tcatgggcaa gaacctgggc
      481 gaggaaggcg gggtcaagcc gaaggacgtc aagtggcacg acgaggcgcc cgaaggaaag
      541 ctcgacctcc tggtcacgct cgactttcgc atgtccacga cgtgcgcctt ctccgacatc
      601 gtgctgccgg tcgctacctg gtacgagaag aacgacctca acacctccga catgcacccg
      661 ttcatccatc ccctgatggc ggcgaccgac ccggcctggg agtgcaagag cgactgggag
      721 atctacaagg ccatcgcccg gcagttctcc gccatcgcac ccgaggtcct gggcgtggag
      781 aaggacgtgg tgctggtgcc gatccagcac gacagcccgg gcgagctggc ccaggcctac
      841 gacgtcaagg actggaagaa gggcgagatc gagccgattc ccggccgcac catgccgacc
      901 gtcgccgtgg tcgagcgcga ctacccgaac gtctacaagc gcttcacggc gctcgggccg
      961 ctgatggaga aggtcggcaa tggcggcaag ggcatcgcct ggcagacggg gcacgaggtc
     1021 gacctcctgc gggcgctcaa cggggcggtg accgaggacg gcccgaccaa gggcatggcc
     1081 cggatcgaca gcgacatcga tgccaccgag gtggtgctga tgctcgcccc cgagaccaat
     1141 ggcgaggtcg cggtcaaggc ctgggaggcg ctctcgaaga tcaccggccg cgagcacgcc
     1201 cacctggcgc tgaccaagga ggacgagaag atccgctttc gcgacatcgc cgcccagccg
     1261 cgcaagatca tctcgtcacc gacctggtcg ggcctggaga gcgagaaggt ctgctacaat
     1321 gccggctaca ccaacgtgca cgagctgatt ccctggcgca cgctctccgg gcgccagcag
     1381 ctctatcagg accacgactg gatgcgggcc ttcggcgagg ccttcgtcac ctggaagccg
     1441 ccgatcgatc cgaaatccgt ggcccgcatc cagggcaagc tgcccaacgg cagcaccgag
     1501 atcgtgctca acttcatcac gccgcaccag aagtggggca tccactccac ttacaccgac
     1561 aacctcctga tgctgacgct cagccggggc gggccgatcg tctggctcag cgaggacgac
     1621 gccaagaagg ccgggatcgt cgataacgac tgggtcgagg tctacaacac caatggcgcg
     1681 ctcaccgccc gggcggtggt ctcgcagcgg gtgaatcccg gcatgatgct gatgtaccat
     1741 gcccaggaga agatggtgaa cacgccgggt gccgagacca ccaagaagcg cggcggcatc
     1801 cacaactcgg tgacccgggc gatcctcaag ccgacccaca tgatcggcgg ctacgcgcag
     1861 ctctcttacg gtttcaacta ctacggcacg gtcggctcca atcgcgacga gttcgtcatc
     1921 gtccgcaaga tgcagaacgt cgactggctc gaggggccgc tgaaccgccc cgccctcgag
     1981 gaaacgtcgc aacccgtcgg tgtggaggcc gcagaatgaa gatccgcgca cagatcgcga
     2041 tggtgctcaa cctcgacaag tgcatcggct gccatacctg ctcggtgacc tgcaagcagg
     2101 tgtggaccaa ccgcgaaggc atggaatacg cctggttcaa caatgtcgag accaagcccg
     2161 ggatcggcta tcccaaggaa tgggagaacc agaagaagtg gaacggcggc tgggtccgca
     2221 agtcgaacgg caagatcgag cccaggatcg gcggcaagtg gcgggtgctg gcgaagatct
     2281 tcgccaaccc ggacctgccc gagatcgacg actactacga gcccttcacc ttcgactacg
     2341 accacctgca gagcgccccg gagctcgagg cgacaccgac ggcccggccg cgctcgctga
     2401 tcaccggcga gcggatggag aagatcgagt gggggccgaa ctgggaggag atcctcggcg
     2461 gcgagttcgc caagcggtcg aaggacgtga acttcgaggg cgtgcagaag gagatctacg
     2521 gcgccttcga gaacaccttc atgatgtacc tgccgcgcct ctgcgagcac tgcctcaacc
     2581 cgacctgcgt cgccgcctgc ccgtcgggtg ccatctacaa gcgcgaggag gacggcatcg
     2641 tcctgatcga ccaggagcgc tgccgcggct ggcggatgtg cgtctcgggc tgcccctaca
     2701 agaagatcta ctacaactgg gcatcgggca aatccgagaa gtgcatcttc tgctacccgc
     2761 gcatcgaggc cggccagccg accgtctgct ccgagacctg cgtcggccgc atccgctatc
     2821 tcggcgtcgt gctctatgac gccgacggga tcgaggcagc ggcggccacg accaacgagc
     2881 aggacctcta cgaggcgcag ctcgagctct tcaaggaccc gaacgacccg gaggtgatcg
     2941 cccaggcccg ggccgacggc gtgccggaag cctggctcga gggtgcccgg cgctcgccga
     3001 tctggaagat ggcgatggac tggaagatcg ccttcccgct ccatccggaa taccggacca
     3061 tgccgatggt ctggtacgtg ccgccgctct cgccgatcca ggcggccgcc gaggccggcc
     3121 agatgggcgt cgacggcgac atgccggacg tccgctcgct tcgcatcccg ctgaagtacc
     3181 tcgccaacct gctcaccgcc ggcaaggagg agccgattgc gctcggcctc gagcggatgc
     3241 tggcgatgcg tgcctacatg cgggcgaaga ccgtggacgg cgtgatcgac gaggcgatcg
     3301 ccgagcgcgt cggcatgaag ggtctggaca tcgaggagat gtacgagctg atggccatcg
     3361 ccaattacga ggaccgcttc gtcattccga cctcgcaccg cgagaccgga gtcgatccct
     3421 acgagctgcg cggcagttgc ggcttcacct tcggcaattc ctgctcgggc gggaccagcg
     3481 acgcgacgct gttcttcggg gcgcccagcc gcaagaagcc gaagacgccg atggaggtgt
     3541 cgtgatggcc agcagcggag cccgttccta caaggcgctc tcggccctcc tcgcctaccc
     3601 gacggccgag ctgcagggcg cctgcggcga gatccaggcc gtgctccacc aggaacggct
     3661 ggtgccggcg cacaccctgc ctgccctcga ccggctgatc gccgagctcg cctcgctcga
     3721 tctctacgag atccaggagc gctacaccgt gctcttcgac cggtcgcgct cgctctcgct
     3781 ccatctcttc gagcatgtcc acggcgagag ccgcgaccgc ggccaggcga tggtcgacct
     3841 cgccgcccac tacgagcggc acggctactg gatccgggcg aacgagctgc cggacttcct
     3901 gccgctcttc ctcgagttcc tctccctcct gccgcgcgac gaggcgcacg ggctgctgca
     3961 gcagccgctg ccgatcctcg aggccctgga ggagcggctg cggcgccgcg gctcgaccta
     4021 tatcgcgatc ttcgtggccc tcttggcgat cgccgggagc aaggccgcat cggtgccgct
     4081 ggcgaccacc gccgatgacg atcccgacga cctcgaggcg ctcgacgccg actgggagga
     4141 ggcggccgtc accttcggcc ccggcggcgc cgccgatgcc gacgacgcct gcggcaagag
     4201 ccggctcgcc acccgcatcc gcgccgagat ccgcagcgtc cacgatcgtc ctgcctgagg
     4261 agaaacccca tggcgacctg gtacaacacc ttcttcttcg gcatctatcc ctacatcgcg
     4321 ctggcggtga tgatcgcggg ctcgatcatc cgctacgacc gcgagcccta ttcctggcgc
     4381 tcgggctcga gccagatgct gcgcaagaag cagctcttct ggggctccaa cctcttccat
     4441 ttcggcatcc tcttcctctt cttcggccac ctggtcggcc tgctgacacc gcactggggc
     4501 tattcctggg ccttcgatgc cagcgtcaag cagctcgtcg ccgtgatcgc cggcgggatc
     4561 gccggggtgg tctgcttcgt cggcctcacg ctgctgctgc atcggcggct gaccgacccg
     4621 cgcatccgct cgaccagcac caccatggat atcgccatcc tcgtcatcct ctgggtgcag
     4681 ctctcgctgg gcctcctgac cctgcccttc tcgctctcgc acagcgacgg cgggacgatg
     4741 atgaccctgg ccgaatgggc gcagcgcatc ctcaccttcc gcggcgacgc ggcgatcctg
     4801 gtggcgggcg tcgactggcc ctatcaggtc cacatcattc tcggcctcac catcttcctg
     4861 ctgttcccgt tcagccgcct cgtccacatc tggtcggtgc cggtccgcta cgtctggcgg
     4921 ccgggctatc aggtggtccg gagcgccggc gccgactggc gggtcccggg gccggtcggc
     4981 cggccgcgga tcgaggcccg ggccgagccg atggagccgg ccgaatgagc ggcatgcgca
     5041 tcttcgatct ggagagccgg ccgaagctgc gccagccccc gccggtcacc gtcaacggca
     5101 cggtgatcga ccaggcggcc atcgcccgcg aggcgcagca ccatccggcc ggctcggccc
     5161 gcgaggccgg cgaggcggcg gcgcgggcgc tggtgatccg ccatctgctg ctcgaggagg
     5221 cggcacggct cgagatcgag ggcgtgcccg agatcgacga cgagggccgg cgcgagacgc
     5281 cggaggaggc gcggatctcc accctgctgc ggcaggaggt ccaggtgccc gaggccgacg
     5341 aggcggcggc caggcgcttc tacacgcaga accagaggcg cttcaccagc ccggacctct
     5401 acgaggcggc gcatatcctg ttctcggccg atgccgcgga cccggtggcc tatggacggg
     5461 cccgtgagca ggcgctggcg gccctcgccc gcctggccga gcagccgcaa cggttcgccg
     5521 ccatggcgcg cgacgtctcg gcctgcccgt ccgccgcctc gggcggcaat ctcggccagg
     5581 tcaccggcga tgcggtgacg ccggcgttcg ccgaagcgtt gcgacgggcc gagcccggcc
     5641 gggtccatcc ggagccggtc gagaccccat atggcgtcca cgtgctccgg ctcgagcggc
     5701 ggatcgccgg ccgggtgctg ccgttcgaga tggtcgagga gcggatccgg gaatggctgg
     5761 gcgcccgggt ctggcgccat gccgtgcgcc agtacgtgtc gatcctggtc ggccgggccg
     5821 agatcggcgg catcgagctc ggcggcgccc agtcgccgct ggtgcagtag cggagtcggc
     5881 ggatgatggg tgagctggtc ggtcgtttga ccgagcccgg cgtggcggag gccacgctga
     5941 tcgaggcggg cgagctcggc ctgctcgccc gcctgagcaa tgccgccggc atgctcgatg
     6001 ccgatatcgg cgacctcacg tcgcacatcg tccggacctg gctcgcccgg gccgacgacg
     6061 aggcctgggt ccagctgatc ggcgtcatga accggagcgc ggtgccgggt ctggcagccc
     6121 tcgtcctgat ggtcgagcag gcgctggccg agctcgacac gacgacggcg cactgaaagc
     6181 ccgcacgaga gcagccatgc cggttgatcg gccgcgcgaa agcctcgacc ccctgcccgc
     6241 gggcctcctg cgccagccgc tcgactacct catggccgac catcatcgtc agcgtgtgct
     6301 ctgccggctg gccgacgcgc tggcggcgac gtcggggtcc gatcccgacc tcgcccggac
     6361 cgtcggcgcg ttcatcagcc gcgacatggc gatccacgtg atcgacgagg agcaggacct
     6421 gttcccgctg ctgcgtcgtc gggcgtccct cgaggacgcg atcgacgccg tgctcggccg
     6481 gctcgccgcc gagcacagca gcggcagcgc gctggcggcc cggatcggcc gggcgctcga
     6541 cgaggcagcg ctcaccggcc ggccgctgga ccgctcgacc cgactcgatc tcgccgagtt
     6601 cgcgcgcagc gagcgcgagc acctggcgct cgagaacgcc gtcgtcatgc cgctggcacg
     6661 gctgcgtttg acggccggcg atctgcgtgc tctgtccttg cgcatggccg cgcgccgcgg
     6721 cctgctcatc gatgacccgg gccccaccgc cgcatgatcg acgacctctt ccgccgcaac
     6781 cgcgcctggt cgatcgcccg ccaggagcgc gatcccgatt tcttcccgcg gctcgcccgg
     6841 caacagcgcc cggcatatct ctggatcggc tgcgccgaca gccgggtgcc ggcgaacgag
     6901 atcgtcgggc tggacccggg cgagctgttc gtccaccgca atgtcgccaa cctggcgccg
     6961 ccctccgaca tcaatttcct ctccgtcctc tccttcgcgg tggccgtgct caaggtgcag
     7021 cacgtcatcg tctgcggcca ttacggttgc ggcggggtcc gtgcggccct gctcgacgag
     7081 cgcaacggca tcgtcgatca ctggctggag ccggtgcgcg gcacctatcg gcggaacgcc
     7141 gcgcggatcg aagcgatcgg cagcgaggcc gaccgggtca acgcgctctg cgagttcaac
     7201 gtccaggacc gggtccagga cgtggccgcc acgacgatca tccgcgacgc ctgggctgcc
     7261 ggccaaaagc tcgccatcca cggctggatc tacggcctcg ccgacggcct gctgcgcaat
     7321 ctcgacgtga cagtggcggg gctggaggat tcgtagcagg cgcccgctag agaggcggca
     7381 gcggcgaaac gccgaagagc gccgggtgga cgaggagcag ggctacccag atcagcagag
     7441 cgagcgccac gcggcccgcg ggaaaggcat cgccgggcgc cggcagcggg gcgccacccg
     7501 gcaggaacgc cgtcttgcgc gtgagcgcct gccagcgccg atcgccgcat cgccgcctgt
     7561 gccgccgatc gagcaggatc atgccgagga cggccaggac ggcgaggccg ccgaacagca
     7621 gggcgtgggc gagatcaccg ttggggacca tatgggcgat ggcccagagc gccacggcca
     7681 gcagcagcgg gtgacgcgag acagccgtga tgccgggtcg tgccggatcg aattcgtgcc
     7741 ggcggctgcc gccgagcgag aagggattgg ccaccgccgt gccgcagacc gcgagcacca
     7801 gggcgagggg catcaccagg ttcggaatcc aggcctgcca gccggccggg tcccagagcg
     7861 gcacataggg tgcccgcccg gctgcgacca gcagccagta gagcaggagg agcgagagca
     7921 gcgagtagag cacgagatag ccccgctcgc cgagggctcc ggtcagccgg gcgcgcagcg
     7981 tggtgcgcgg cggaacggcg tgggcgacga cgaagaggag gacggcgagg ccgtactccg
     8041 cccagccggt caaggtgcca tggccacgcg cggcgcgggc ggtggcgcca cggcgcaatc
     8101 ggcgaccgtc accgtctcga gctcggtgag gaaggcctcg cgggcactgg ccagatgctt
     8161 cttgagccgg cagcccggcg tcagcacgca ggcgccgccg tcggcacgaa agcactcgac
     8221 caaagcgtgc cgcccctcga gctgccggac gacgtcgccg accgtgatgg cgcccgccgg
     8281 gcgggcgaga cgaaagccgc caccggcgcc gcgggcggtg ctgacgaagc cggccgcgcc
     8341 gagcgcccgc accaccttgg tcagatggtg gcgcgagacg gcgagctccg tcgccagcgc
     8401 ctcggtggtg atcacccggc cgggcgagcc ggcgagccgc atcagcaccc tgaggccgaa
     8461 atcagtgaac gcggtgagac gcatcgccgc tccattctga attggtattg aaaatactgt
     8521 ttaatcgaag tggactgcag tccgcaatcc cgtcggccag ccgaggccgg ccagcccttc
     8581 cgggaaaccc gcatgagcgc cacgaccgcc gagcgaatcc gcacctaccg gggccccgcc
     8641 gtgctcggcc tcggcttccg cccgttcttc ctcgccgctg gcctgtggtc ggccctggcg
     8701 atgctgctct ggatcctgct cttgaccggc ggcatcgacc tgccgatcgc cctggcgccc
     8761 gtcgactggc acgttcattc gctcctctac ggcttcgtcc cggccaccgt caccggcttt
     8821 ctcctgaccg ccgtgccgaa ctggacggga cggctgccgg tcaccggcac gccgctgctc
     8881 tgcctggcgc tcctctggct cgccgggcgc ctcgccgtgc tctttggtga cgttctcggc
     8941 ccacttctga gcgccctcgt cgatctcgcc ttcctggcgg ccgtcttcgc cgtcatcctc
     9001 cgcgagcttc tggccggccg caacaagcgc aacctgccgg tgctggcgct ggtcggcctg
     9061 ctcttcctcg gcaacgcgct cttccatctc gaggccgccc gcaccggtgg ggcctactat
     9121 gggacgcggg tcggcatcct cgccatcttg atgctgatca tgctgatcgg cgggcgcatc
     9181 gtgccgagct tcacccgcaa ctggctggtc aagcgcgggg cgggccggct gccggcctcc
     9241 ttcgggcgct tcgacggggt ctgcctcgcc accgccgcca cggcgctggc ctcctgggtc
     9301 gtcgcgtcgg aggccgcggc gacggcctgg ctcgccggaa tcgccggagc gctccacctc
     9361 gtccgcctcg gccgctgggc cggcgagcgg accacggccg agcccctgct cctcgtcctg
     9421 cacgtcgcct atgcgttcgt gccgctgggc ttcttcctcg tcctcctggc gcacgtcctg
     9481 ccgtcgaccg tgctgccggt ggcggccatc cacgcctggc tcgccggtgg tgtcgggctg
     9541 atgacgctgg ccgtcatgac ccgcgccagc ctcggccatt ccggccgggc cctgaccgcg
     9601 gactggccga cggtcgccat cttctgcgcc ctggtcgtgg cggccctggc acgcatcctc
     9661 gccggcacca cgctcgccct gcccggcctc ctgcatctgg cggcgaccgc ctggatcctc
     9721 gccttcggcg gcttcgccgt cgcctactgg cccgtgctga cgggcgttgc cgccgggctc
     9781 aagccgtcgt cgccgcctcc aggaagaccc cgttgatctt ccagctgccg tcgggctgct
     9841 gctgcatctg gtagtaggcg gtcaccgtct cccccttcgg gcccaccaga tgcaccatct
     9901 gggccggctg gccccgatag gtcacgacag cgccgaacgt gacgctgcgc gggcggtaga
     9961 tctgcggata gccctggacg accatcgcca tgaagctctc gggcgtgccg aagaggctcc
    10021 tgatggtcgg cgaggcgaag gagaaggcgg ccccgccatc gtcgtgccgg aaggcctcga
    10081 gctgcgcctc gatcaccgcc ctgaaggcat cgggctcgcc ttccgcccgg gcggcctgat
    10141 ggccggtgag caggagcaga acgagcaagg aggaagcgag caggcgcatg gccgtgtctc
    10201 cactggcacg aggat
//
LOCUS       DAOTFI010000181        10212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7796_length_10212_cov_6.860687, whole genome shotgun sequence.
ACCESSION   DAOTFI010000181 DAOTFI010000000
VERSION     DAOTFI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_10212_cov_6.860687"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="P5558_16940"
     CDS             <1..1280
                     /locus_tag="P5558_16940"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY26057.1"
                     /translation="MEKFAGPLVARDNPVLRRLLVDEVPETFRELCAAGIEFMGPIPE
                     PPHRHPRLHQIVPHSRGYIHHLAKACRRAGAELRTGWRVGRLRQDGGRISGLDATRAD
                     GGSVTVHSRQAVILAAGDFSAADPAFKARFLAPELLEVEGTNPTSTGDGQRLGEVVGG
                     EIVNGDLVWGPEIRFLAPPRTALVNRLPPWRTTARAIRLAMKVVPDRLLRPFLMSFVT
                     TFLAPSPGLFDAGAILVNKAGARFCDELDRPQDRISREADGVSYLVFDAAIAERFEAW
                     PGFVSTAPGVGYAYLQDYLRSRRDITFRAETVEGLAAAMGVDGTALARTVADYNASPE
                     RGERPALTRPPFLALGPAKSWIMFGEGGLRVDQRLRVLDGERRPIAGLYAAGSCGQGG
                     LILNGHGHHLGWAFTSGRLAGQNAALGEAPQGN"
     gene            1343..1861
                     /locus_tag="P5558_16945"
     CDS             1343..1861
                     /locus_tag="P5558_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY26058.1"
                     /translation="MDKAKERLGDARILGPDDGESHWQPVPANGHVRCLFSDSTVKST
                     NRFAMGTQTVAPGCFIREHTHDRNEEIIFVVSGRGFVKLDGEEHPIEPGSAVYLGVNR
                     KHMFINPGPDPLTFVWYFTPGGLDDFFAQIGRPRTPGEPTPEPFARPDNVAEIEQRTV
                     FGWTDQDFASRG"
     gene            1884..2918
                     /locus_tag="P5558_16950"
     CDS             1884..2918
                     /locus_tag="P5558_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRY26059.1"
                     /translation="MRFKRMVTVVGAHAEGELNEVITGGVVDVPGRTMFDKMRHLETE
                     ADDLRNFLLNEPRGKVQQCVNLVLPSSHPDAAMGFVIMESDYYVPMSGTNTICTVTVL
                     LETGMIPMQEPVTKVTLEVPAGLIEVTASCRNGKCERVAFRNVPSFVFGLDLPLEVEG
                     LPTLTVDVAYGGMIYVLVDAEALGFAVTSDEGAAMVETGERIKAAAAVQIPAVHPENP
                     LIHTINQTLFAAPLRDEAGGKRARNAVVVSPGRLDRSPCGTGTSSRLAVMHARGQIAV
                     GEAFTHESVIGTTFGGKVLETTTLAGKPAIVPEISGQGWITAFHQYVLDPTDPFPTGY
                     RVGDMWRNSG"
     gene            complement(2922..4445)
                     /locus_tag="P5558_16955"
     CDS             complement(2922..4445)
                     /locus_tag="P5558_16955"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY26060.1"
                     /translation="MAVADQLLAFAAAIGHLLSQPTLVVVLMGSSLLGIVFGALPGLT
                     ATLGVGLLTTLTYGFDLDLALVSLIALYVGAIYGGSYSAILLNIPGTAASAASAMDGY
                     PMARAGEGGRALGLTTTASFIGTLLGMLALVLTAPLIVELALQFTSYEFFLLALFGII
                     ISGSLTSPDLVIKGWIAGFLGLALACIGRDKLMYYPRYTFDIHALDSGIEVVPVLIGA
                     FAIPQIIGTLRSKAPLAAVQAVGRIIPDFRTIARNLRHIVRSALIGVGIGAVPGVGED
                     IAGWVSYGAAKNGSPHPERFGQGEPAGLISSETANNACIGGALIPLVTLGIPGSPPAV
                     MLLGALMLHGLTPGPMLSIQQPTFLVELAAIMVLASAAMWLNGIVLARQVVKLLRVPA
                     TLFLPIVALLAVLGSYALGLNLTNLYLMVAVGIAAYVLTEMDYPIAPLVIGVILGPMA
                     DENLRRALMVSQGDPLDFFTRPVSLVLIAAILLTVLAQVPAWRRWRQSLLARFRRRG"
     gene            complement(4449..5201)
                     /locus_tag="P5558_16960"
     CDS             complement(4449..5201)
                     /locus_tag="P5558_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26061.1"
                     /translation="MTTSPSSAATRGRPSDLWTGLALLLIAAAMAGGALQFPLRGTYA
                     GVKNAWYVSPALFPLIVATCLCLLAVTLIVTTVRSGVLADRGSLASALAGQGDVVLIA
                     GIIAAFVVGLVPRVDFVVAGFFFLLAFTLAFHVHEPRVAKLALGAFFATTLVLLVMAG
                     LGLTPVVRTAAASLVDLAVLLVAVGTFVAARAMTTDATARHRMGQCLAVALATPLLLG
                     ITFKFFLLVPLPREGLVVVALETVYYAIRGAL"
     gene            complement(5312..6316)
                     /locus_tag="P5558_16965"
     CDS             complement(5312..6316)
                     /locus_tag="P5558_16965"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY26062.1"
                     /translation="MRATTTLALGIAAALTATGAVAQDYPSRDINAVVVWSAGGGTDT
                     ANRLILAEMQENLPVRINVTNRTGGVGGSVGMSYVFEQPADGYTLAGISESVVTAGVQ
                     GGWDQRMDVWYPFIIGGSPDLVSVSSDAPWQSLAELIEAAKATPGEIRAGASAAGSIH
                     HLNLLALEKGTGATFNYIPYDGSAPAQNAAVAGEVQVIVTSLAEQQQLLQAGRLRPLA
                     MLVPTGVELNGEAIPSAFDSYPELGEHLPIKQAIGFAVLDAAPDEVKATLVEAFDAAM
                     ASDKVQTWLDENYYEASGASGDAAKEEFARLESLFAWTLHDLGAASVDPATLDIPKPN
                     "
     gene            6447..7457
                     /locus_tag="P5558_16970"
     CDS             6447..7457
                     /locus_tag="P5558_16970"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY26063.1"
                     /translation="MLDPAWLRPLGSGLARPECVLAHRSGLLFAADWTGQGGVAIVHP
                     DGRVDRHLATNAPRHLRPNGIALEPGGSFLLAELGADTGGIWRLQPDGSVEPVLLEVE
                     GRPLPPANYVHQDVEGRLWVTVSTRHIPRHEAARPDVADGFVVLVADGNARIVADDLG
                     YTNECLVSPDGQWLYVNETFGRRLSRFPLGARGLGERRTVAEFGHGTYPDGLTFDEDG
                     HVWVTSIISNRVIRVDPEGRQELMLEQVEEDHVAAVESAYRAGELDRTLLDRVPAGPL
                     KNISSLAFTGADRGTICLGCLLGDSLLLVESPVAGAAPVHWEHKLGGLWSQLGDRLED
                     DQ"
     gene            7454..8479
                     /locus_tag="P5558_16975"
     CDS             7454..8479
                     /locus_tag="P5558_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY26064.1"
                     /translation="MKKLRVATIGAGYFSAFHHDAWQRIPEVELVGVCDRDPAKAEAY
                     AERFAVPSWHTDPATMLDELRPDLVDIATPPATHAELIRLTMARRLPTICQKAFCRSL
                     DEAEEMVGVAEAAGTLLVVHENFRFEPWHMEIKRQLEAGRIGEVFQASFRLRPGDGQG
                     PRAYLDRQPYFQQMGRFLVHETAIHFIDVFRFLLGEPEAVLARLVRLNPAIAGEDAGV
                     ILFEFPGNRRALFDGNRLVDHPARNRRLTMGELLVEGDEGVIRLDGDGNLFHRAFGAN
                     EETAIAHEWQDTGFAGDSVRAFQQHVVDHLLDKGEIVNTGRDYLANLRIEEAVYRSSA
                     EGRLVRL"
     gene            8481..9167
                     /locus_tag="P5558_16980"
     CDS             8481..9167
                     /locus_tag="P5558_16980"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY26065.1"
                     /translation="MASVIDEAPGRVTFAERAYREIKARILENEFPAGSIMLEQELAA
                     LLEMSRTPVREALIRLAREGMIDVRPRHGMRVLPVSANDMREIYQILTALEAEAAAQI
                     ARDGATPARLAELKAAVAEMDAALAADDLDAWALADDHFHRVILAFCGNERLKAIIEQ
                     FMDQSHRVRKLTLRLRPRPVSSNDDHRALVAAIERRDAETAWRIHHAHRLRNGRMLVE
                     LLERHGLGQL"
     gene            complement(9220..9729)
                     /locus_tag="P5558_16985"
     CDS             complement(9220..9729)
                     /locus_tag="P5558_16985"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HRY26066.1"
                     /translation="MRRKSWIIRTVIGWHKVRFQIEKRINLTATQSRSHEIDHVKKFA
                     AGTIALEIEWKNKDSFFDRDLENFNRLHADGGISVGIVITRGRSFQDGIEKRLRRFVR
                     DQNIDAFAALVAHGVDPTRRQRRAVERGGALPTSGQGVSLRTSSGRPQLIGPSLTTAS
                     IAASAAPVL"
     gene            complement(10001..>10212)
                     /locus_tag="P5558_16990"
     CDS             complement(10001..>10212)
                     /locus_tag="P5558_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26067.1"
                     /translation="TIQQRQGFQIACPEEIAHLLGHIDDDQLRQLAGPLAKTAYGRYL
                     LDVVLAKEPWQAGSFETSSARRAVA"
BASE COUNT         1678 a         3415 c         3639 g         1480 t
ORIGIN      
        1 acatggagaa gttcgccggc ccgctggtgg cgagagacaa cccggtgctc cggcgcctgc
       61 tggtcgacga ggtgccggag accttccgcg agctctgcgc ggccggcatc gagttcatgg
      121 ggccgatccc ggaaccgccg catcgccacc cgcgcctgca ccagatcgtg ccgcattcgc
      181 gcggttacat ccaccacctg gccaaagcct gccggcgggc cggggcggag ctgcgcaccg
      241 gctggcgcgt cggccgactg cgccaggatg gcggccggat cagcggcctc gacgcgacac
      301 gggcggatgg cggcagcgtc accgtccaca gccggcaggc ggtgatcctg gcagccggcg
      361 atttcagcgc ggccgatccg gccttcaagg cgcgcttcct ggcgccggag cttctcgagg
      421 tcgagggcac caacccgacc agcaccggcg acgggcagcg gctcggcgag gtggtgggcg
      481 gcgagatcgt caatggcgat ctcgtctggg ggccggagat ccgcttcctc gccccgccgc
      541 ggaccgccct ggtcaaccgg ctgccgccct ggcgcacgac cgcgcgggcg atccgacttg
      601 ccatgaaagt cgtgcccgac cgactgctcc ggccgttcct catgtccttc gtcaccacct
      661 tcctggcccc gtcgcccggc ctcttcgacg ccggagcgat cctcgtgaac aaggcggggg
      721 cgcggttctg cgacgagctg gaccggccgc aggaccggat cagccgggag gcggacgggg
      781 tctcgtacct cgtcttcgat gccgccatcg ccgagaggtt cgaggcctgg ccgggcttcg
      841 tctcgaccgc gcccggcgtc ggctatgcct atctgcagga ctatctgcgc tcgcggcggg
      901 acatcacctt ccgggcggag acggtggagg ggctcgccgc ggcgatgggc gtcgacggca
      961 cggccctcgc caggacggtc gccgactaca acgcctcgcc cgagcgcggc gagcgcccgg
     1021 cgctgacgcg gccgcccttc ctcgccctcg ggccggccaa gtcctggatc atgttcggcg
     1081 agggcgggct gcgggtcgac cagcggctgc gcgtgctcga tggcgagcgg cggccgatcg
     1141 ccggcctcta tgccgccggc tcctgcggac agggcgggct gatcctcaac ggccacggcc
     1201 accatctggg ctgggccttc acctccggcc ggctcgccgg ccagaacgcg gcgctcggcg
     1261 aggcaccgca gggcaattga cgaccggatg cgcctatgct ttcctgactc atatgtcagt
     1321 agccgtgcag ggggagaatg ccgtggacaa ggcaaaagag aggctgggcg acgccaggat
     1381 cctggggccg gatgacggcg agagccactg gcagccggtg ccggcgaacg gccatgtgcg
     1441 ctgcctcttc agcgacagca cggtcaagag caccaaccgc ttcgccatgg gcacgcagac
     1501 cgtggcgccc ggctgcttca tccgcgagca cacccacgac cggaacgagg agatcatctt
     1561 cgtcgtttcg ggccggggct tcgtcaagct ggacggcgag gagcacccga tcgagcccgg
     1621 ctcggccgtg tacctcggcg tgaacaggaa gcacatgttc atcaacccgg ggccggatcc
     1681 gctcaccttc gtctggtact tcacgccggg cgggctcgac gacttcttcg cccagatcgg
     1741 ccggccgcgc acgcccggcg agccgacgcc cgagcccttc gcccggcccg acaacgtcgc
     1801 cgagatcgag cagcgcacgg tcttcggctg gaccgaccag gacttcgcca gccgcggctg
     1861 agaatcgagg gaggcagcgg gcgatgcggt tcaagcgcat ggtcaccgtc gtcggcgcgc
     1921 atgccgaggg cgagctcaac gaggtgatca ccggcggcgt ggtcgacgtt cccggccgca
     1981 ccatgttcga caagatgcgc catctcgaga ccgaggcgga cgatcttcgg aacttcctct
     2041 tgaacgagcc gcgcggcaag gtgcagcaat gcgtcaacct cgtgctgccg tcctcgcacc
     2101 ccgacgccgc catgggcttc gtcatcatgg aatcggacta ttacgtgccg atgtccggga
     2161 ccaacaccat ctgcacggtc accgtcctgc tcgagaccgg catgatcccg atgcaggagc
     2221 cggtgaccaa ggtcacgctc gaggtgccgg ccggcctcat cgaggtcacg gcgagctgcc
     2281 ggaacggcaa gtgcgagcgg gtggctttcc gcaacgtgcc gtccttcgtc ttcggcctcg
     2341 acctgccgct cgaggtcgag gggctcccga cgctcacggt cgacgtcgcc tatggcggga
     2401 tgatctacgt gctcgtggac gccgaagccc tgggcttcgc cgtgacctcg gacgagggcg
     2461 ccgccatggt cgagacgggc gagcggatca aggcggcggc cgccgtccag atcccggccg
     2521 tgcatcccga gaacccgctg atccacacca tcaaccagac gctcttcgcc gcccccctgc
     2581 gcgacgaggc gggcggcaag cgggcgcgca acgcggtcgt ggtctcgccc ggccggctcg
     2641 accgctcgcc ctgcggcacg ggcacgagct ccaggctcgc cgtcatgcac gccaggggcc
     2701 agatcgcggt cggcgaggcc ttcacccacg aatcggtgat cggcacgacc ttcggcggca
     2761 aggtgctgga gacgacgaca ctggccggca agcccgccat cgtgcccgag atctcgggcc
     2821 agggctggat caccgccttt caccagtacg tcctcgaccc gaccgacccc ttcccgaccg
     2881 gctatcgggt cggcgacatg tggcgcaatt cgggctgagg ctcagcccct gcggcggaac
     2941 cgggccagga ggctttgccg ccagcggcgc caggcgggga cctgggcgag gaccgtgagc
     3001 aggatggcgg caatgaggac gaggctgacc gggcgggtga agaagtcgag cggatcgccc
     3061 tggctcacca tgagggcacg gcggaggttc tcgtcggcca tggggccgag gatgacgccg
     3121 atgacgagcg gggcgatcgg gtagtccatc tcggtcagca cgtaggcggc gatgccgacc
     3181 gccaccatga gatagaggtt ggtgaggttg agcccgaggg cataggagcc gagcaccgcc
     3241 agcagggcca cgatcggcag gaagagggtc gccgggaccc tgagcagctt caccacctgg
     3301 cgggcgagga cgatgccgtt gagccacatg gcggccgagg ccaggaccat gatggcggcg
     3361 agctcgacga ggaaggtcgg ctgctggatc gagagcatcg gcccgggcgt caggccgtgc
     3421 agcatgaggg cgccgagcag catcacggcg ggcggcgagc cggggatgcc gagggtgacg
     3481 agcgggatga gcgccccgcc gatgcaggca ttgttggcgg tctcggacga gatcaggccg
     3541 gcgggctcgc cctggccgaa gcgctcggga tgcggcgagc cgttcttcgc ggcgccatac
     3601 gagacccagc ccgcgatgtc ctcgccgacg ccgggcacgg caccgatgcc gacgccgatc
     3661 agggccgagc ggacgatgtg gcggagattg cgggcgatcg tccggaagtc cgggatgatg
     3721 cggccgaccg cctggacggc ggcgagcggc gccttcgagc gcagcgtgcc gatgatctgc
     3781 ggaatggcga aggcgccgat caggacaggc accacctcga tgccggaatc gagggcgtgg
     3841 atgtcgaagg tgtagcgcgg gtagtacatc agcttgtcgc ggccgatgca ggcgagcgcc
     3901 aggccgagaa agcccgcgat ccagcccttg atgacaaggt ccgggctggt cagcgagccc
     3961 gagatgatga tgccgaagag ggcgagcagg aagaactcgt aagaggtgaa ctgcagcgcc
     4021 agctcgacga tcaagggagc cgtcagcacc agcgccagca tgccgagcag cgtgccgatg
     4081 aaggaggcgg tggtggtcag gccgagcgcc cgcccgccct cgcccgcccg ggccatcggg
     4141 tagccgtcca tggccgaggc ggccgaggcg gcggtgcccg ggatgttgag caggatggcc
     4201 gagtacgagc cgccatagat ggcgccgaca tagagcgcga tgagcgagac cagcgccaga
     4261 tcgaggtcga agccgtaggt cagcgtggtt agcaggccga cgccgagcgt cgcggtcagg
     4321 cccggcaaag cgccgaagac gatgccgagc aggctcgagc ccatgagcac gacgaccagg
     4381 gtcggctgcg acaggaggtg gccgatggcg gcggcgaagg cgaggagctg gtcggcgacg
     4441 gccatggctc agagcgcccc gcggatggcg tagtagaccg tctcgagggc gacgacgacg
     4501 agcccctcgc gcggcagcgg caccagcagg aagaacttga aggtgatgcc gagcaggagc
     4561 ggcgtcgcca gcgccacggc gaggcactgc cccatccggt ggcgagcggt cgcatcggtg
     4621 gtcatggccc gggcggcgac gaaggtgccg acggcgacca gcagcacggc caggtcgacg
     4681 agcgaggcgg cggcggtgcg caccaccggc gtcagcccca ggccggccat cacgaggagc
     4741 accagcgtcg tggcgaagaa cgccccgagc gccagcttcg ccacgcgcgg ctcgtgcaca
     4801 tgaaaggcga gcgtgaaggc caggaggaag aagaaccccg caacgacgaa atcgacgcgc
     4861 ggcacgaggc cgacgacgaa ggcggcgatg atgccggcga tcagcaccac gtcgccctgc
     4921 ccggcaaggg ccgaggcgag gctgccccgg tccgccagga caccgcttct gaccgtggtc
     4981 acgatcaggg tgacggccag caggcagagg caggtggcga caatgagcgg aaagagggcc
     5041 ggcgagacgt accaggcgtt cttgacgccg gcataggtgc cgcgcagggg gaactgcagg
     5101 gcaccgcccg ccatggcggc cgcgatgagc aggagggcga ggccggtcca gaggtcggac
     5161 gggcggccgc gggtcgcagc actgctgggc gaggtggtca ttcaggcacc tggcggattt
     5221 tcggccggat tccggctcaa gagcctgtcc gagaacggca gacgggcggg aacgacgatg
     5281 cgtccccgcc cgcgcaaggc ccgttcgtgg atcagttggg cttcgggatg tcgagcgtcg
     5341 ccgggtcgac cgaggcggcg ccgaggtcgt gcagggtcca ggcgaagagc gactcgagcc
     5401 gggcgaactc ctccttggcg gcatcgccgg aagcgcccga ggcctcgtag tagttctcgt
     5461 cgagccaggt ctgcaccttg tcgctggcca tcgccgcatc gaaggcctcg accagggtcg
     5521 ccttcacctc gtcaggggcg gcgtctagca cggcgaagcc gatcgcctgc ttgatcggca
     5581 ggtgctcgcc gagctccgga tagctgtcga aggccgaggg gatcgcctcg ccgttcagct
     5641 cgacgccagt gggcacgagc atggcgagcg gccggagccg gccggcctgc agaagctgct
     5701 gctgctcggc gagcgaggtg acgatgacct gcacctcgcc ggcgacggcg gcattctggg
     5761 ccggtgcgga gccgtcatag gggatgtagt tgaaggtggc gccggtgccc ttctcgagag
     5821 cgaggaggtt caggtgatgg atcgagccgg cagcgctggc gccggcgcgg atctcgccgg
     5881 gcgtggcctt ggcggcctcg atcagctccg cgagcgactg ccagggcgcg tcgctcgaga
     5941 ccgagacgag gtcgggcgag ccgccgatga tgaacgggta ccagacgtcc atgcgctggt
     6001 cccagccgcc ctggacgccg gccgtgacca ccgactcgga gatgccggcc agcgtgtagc
     6061 cgtcggccgg ctgctcgaag acgtagctca tgccgaccga gccgccgacg ccgccggtgc
     6121 ggttggtgac attgatgcgg accggcaggt tctcctgcat ctcggccagg atcaggcggt
     6181 tggcggtatc ggtgccgcca cccgccgacc agacgacgac ggcgttgatg tcgcggctcg
     6241 gatagtcctg ggcaaccgca ccggtggcgg tcagggccgc ggcgattccc aaagcgagag
     6301 ttgttgtggc gcgcatggtc tgctcccttg gtcgaccgta tgtcactcct cgagccgcca
     6361 tccggacgag tcgcttgatt atcggatgcg gttcggtatt tctttacgta tacgtaaatg
     6421 gggaagccgg tcaatgcgca attccgatgc tcgatcccgc ctggcttagg ccgctcggca
     6481 gcggcctcgc gcgcccggaa tgcgtgctgg cgcatcgctc gggcctgctc ttcgccgccg
     6541 actggacggg ccagggcggg gtggccatcg tccaccctga cggccgggtc gaccggcacc
     6601 tcgccacgaa tgccccgcga cacctccggc cgaatggcat cgccctggag cccggcggca
     6661 gctttctttt ggccgagctc ggggcagaca cgggcggcat ctggcggctg cagcccgacg
     6721 gctcggtcga gccggtcctg ctcgaggtcg aggggcgccc cctgccccct gccaactacg
     6781 tgcatcagga cgtggaaggc cggctctggg tgacggtgag cacgcgccac atcccgcgcc
     6841 atgaagcagc gcggccggac gtcgccgacg gcttcgtcgt gctcgtggcg gacggcaacg
     6901 cccggatcgt ggccgacgat ctcggctaca ccaatgaatg cctggtctcg ccggacgggc
     6961 agtggctcta tgtcaacgag accttcggcc ggcggctgag ccgcttcccc ctcggcgccc
     7021 gcggcctggg cgagcgccgg accgtcgccg aattcggcca cggcacctat ccggacgggc
     7081 tcaccttcga cgaggatggc catgtctggg tcaccagcat catcagcaac cgggtgatcc
     7141 gggtcgaccc cgagggcagg caggagctca tgctcgagca ggtggaggag gatcacgttg
     7201 cggcggtcga aagcgcctat cgggcgggcg agctggatcg caccctgctc gaccgcgtgc
     7261 cggcggggcc cttgaagaac atctccagcc tcgccttcac cggcgccgac cgcgggacga
     7321 tctgcctcgg ctgcctgctc ggcgacagcc tcctgctggt cgagagcccg gtcgccggtg
     7381 ccgcaccggt gcattgggag cacaagctcg gcgggctctg gagccagctc ggcgaccgcc
     7441 tggaggacga ccagtgaaga agctcagggt cgcgaccatc ggtgccggct atttcagcgc
     7501 cttccatcac gacgcctggc agcggatccc cgaggtcgag ctggtcggcg tgtgcgaccg
     7561 cgacccggcc aaggccgagg cctatgccga gcgcttcgcc gtgccgtcct ggcacaccga
     7621 cccggcgacc atgctggacg agctccgccc cgacctggtc gacatcgcca ccccgcccgc
     7681 gacccatgcc gagctcatcc gcctgaccat ggcgcggcgc ctgccgacca tctgccagaa
     7741 agccttctgc cgctcgctcg acgaggccga ggagatggtc ggcgtcgccg aggcggcagg
     7801 gacgctgctg gtcgtccacg agaacttccg cttcgagccc tggcacatgg agatcaaaag
     7861 acagctcgag gccgggcgga tcggcgaggt cttccaggcg agcttccgcc tgcggccggg
     7921 cgacggccag ggtccccgcg cctatctcga tcgccagccc tatttccagc agatggggcg
     7981 cttcctcgtc cacgagacgg cgatccattt catcgacgtc ttccgcttcc ttctgggcga
     8041 gcccgaggcg gtgctggccc ggctggtgcg cctcaacccg gccatcgccg gcgaggatgc
     8101 gggcgtcatc ctcttcgagt ttccaggcaa ccgtcgggcg ctcttcgacg gcaaccggct
     8161 ggtcgaccat ccggcgagga accgccggct gaccatgggc gagctgctgg tcgagggtga
     8221 cgagggcgtg atcaggctcg acggcgacgg caatctcttc catcgtgcct tcggcgccaa
     8281 cgaggagacg gccatcgctc atgaatggca ggacacgggc tttgccggcg acagcgtgcg
     8341 cgccttccag caacatgtcg tcgaccacct cctggacaag ggcgagatcg tcaacaccgg
     8401 tcgggactat ctcgccaacc tgcggatcga ggaggccgtc taccgctcct ccgccgaggg
     8461 ccgtctggtg aggctctgag atggccagcg tcatcgacga ggcaccgggt cgcgtcacct
     8521 tcgccgagcg ggcctatcgg gagatcaagg cccgcatcct cgagaacgag ttcccggccg
     8581 gcagcatcat gctggagcag gagctggcag ccctcctcga gatgagccgc accccggtgc
     8641 gcgaggcgct gatccgcttg gcgcgggaag gcatgatcga cgtccggccg cgccacggca
     8701 tgcgggtgct gccggtctcc gccaacgaca tgcgcgagat ctaccagatc ctgacggcgc
     8761 tcgaggcaga ggcggcagcc cagatcgccc gtgacggcgc gacaccagcg cgcctcgccg
     8821 agctcaaggc ggccgtcgcc gagatggacg cggcgctcgc cgccgacgac ctcgacgcct
     8881 gggccctggc cgacgaccat ttccaccggg tcatcctcgc cttctgcggc aacgagcggc
     8941 ttaaggcgat catcgagcag ttcatggacc agtcgcaccg ggtgcgaaag ctgacgctcc
     9001 gcctgcgccc ccgcccggtc agctcgaacg acgaccaccg cgccctggtc gccgccatcg
     9061 agcgccgcga cgccgagacc gcctggcgca tccaccacgc gcaccgcctg cgcaacgggc
     9121 gaatgctggt ggagctgctg gaacggcacg gcctcggcca gctctaggga ccgatgccct
     9181 cgcgatggat gactgcggcg agatgaatgc cgagcgctac tataggacag gggctgccga
     9241 cgccgcgatg gaggcggtcg tcaagcttgg cccaatgagt tgtggccgcc ccgaacttgt
     9301 ccgcaatgaa acgccttggc cagacgtcgg caatgcacca cctcgctcga ccgctctacg
     9361 ctgtcgccga gtcggatcga caccgtgcgc caccagcgcc gcaaaggcgt ctatgttctg
     9421 atcacggaca aaccggcgaa ggcgcttctc aattccatcc tgaaagctgc gaccacgagt
     9481 gatgacaata cctacgctaa ttccaccgtc tgcatgcagt cgattgaaat tctcaaggtc
     9541 gcggtcgaag aaggaatcct tgtttttcca ctcgatctcg agcgcaattg taccggccgc
     9601 gaacttcttg acatgatcga tctcgtggga gcgtgattgg gttgcggtga ggttgatcct
     9661 tttctcaatc tggaatcgaa ccttgtgcca gccaattacg gtcctgatta tccatgattt
     9721 tcgtctcatg gtgtgctagc agaatcaaat ggttggggta gattggaggc gggcacggcg
     9781 atgaagttgg cggccaagcc tgccgacgac cgggatccgt cgctcgcccg aacgtctcct
     9841 gcgccggacg accctctgct ccaggcgaaa tccaggcggg ttcatgggtg gtcgcgatgc
     9901 aggctcgaga ggcctctgtg gccgaagaac gcgcgatgcc aagtcgcgca ggcgtttggg
     9961 cctgcccggc gttgcgtcgg tggggcgccc cgggcggcgg tcaggcgacg gcgcggcggg
    10021 cggacgaggt ctcgaagctg ccggcctgcc agggttcctt cgccagcacg acgtcgagaa
    10081 ggtagcggcc ataggcggtc ttcgcgagag gaccggcgag ctggcggagc tggtcgtcgt
    10141 cgatatgacc caggagatgg gcgatctcct ccgggcaggc gatctggaag ccctggcgct
    10201 gctggatggt gc
//
LOCUS       DAOTFI010000182        10182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7829_length_10182_cov_6.238866, whole genome shotgun sequence.
ACCESSION   DAOTFI010000182 DAOTFI010000000
VERSION     DAOTFI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7829_length_10182_cov_6.238866"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="P5558_16995"
     CDS             complement(<1..505)
                     /locus_tag="P5558_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY26068.1"
                     /translation="MPDSPYPRDMRGYGQHTPHPQWPNGARIAVQFVINYEEGGENCI
                     LHGDPACEAFLSEIVGAQALPGVRHMNMESIYEYGSRAGFWRLRRLFTERRLPVTVFA
                     VAMALERNPEAAAAMVEAGWEIASHGYRWIDYQHVPEEVEREHIRMAVAIQARLTGER
                     PMGFYQGR"
     gene            complement(731..1954)
                     /locus_tag="P5558_17000"
     CDS             complement(731..1954)
                     /locus_tag="P5558_17000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRY26069.1"
                     /translation="MRPTRVVGTNSPGWNELVPMPLFKPAIAPLPDDVRALTALRFFA
                     AFWVLALHYTDFMPADFAAVTGFFAEGKLGVDFFFVLSGFILTHVYLGRLEQGDFAWQ
                     GFMLRRFARLYPLHLATFFVVVAYVTAGRLAGIGFSVPEAYTLEAVWPNLLMLHAWGI
                     QDHMSWNYVSWSISAEWFAYLLFLPLSLLVLRLPFGPGGRLVLAGLGLLVASFVAEQA
                     IGRPLTHLTHDFGILRILPEFVLGITLYHAIRTWDLEARHAGAILAVTLGGILAIVHW
                     RLDGLWAVVLLAFLIFAVASLARQGRLAWLSGPVPVYLGEISYAIYMTHAIVFIVFFR
                     GLGLLLRDPASPHFWWLGPIALALTLLAAMLAHHLVERPARRWLNRLFAGGFRRPSQA
                     PRIHAAPKAHRGAVF"
     gene            2136..2918
                     /locus_tag="P5558_17005"
     CDS             2136..2918
                     /locus_tag="P5558_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HRY26070.1"
                     /translation="MPRFAANLSMMFTDRPFLDRFAAAGAAGFAAVEYLFPYEHEAGE
                     IRAALDDAGLEQALFNLPPGDFARGERGLAALPGREAEFRDGLDKALHYAEVIGARRL
                     HCMAGLIGADDDRAHFSSTYLENLALAAERAAAKGITMLIEPINTRSIPGYFLNYQRD
                     GRRIIERVGAPNLKLQFDCFHCQIMEGDLAMHLRELIGVIGHIQIAGVPDRHEPDTGE
                     VAYPYLFGVMDQIGYAGWVGCEYNPKGRTEDGLGWFGPYRRN"
     gene            2946..3701
                     /locus_tag="P5558_17010"
     CDS             2946..3701
                     /locus_tag="P5558_17010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY26071.1"
                     /translation="MTMTKVAMVTGAGSGIGKASALGLAGAGYKLVLAGRRQEPLAAV
                     AAEIEGTGGQALAVSTDVGDAESVAALFEACAERFGRLDVLFNNAGVGSPPVPIDELP
                     LEQWRKVVDANLNGAFYCSREAFGMMRKQKPQGGRIINNGSISAHVPRPFSAPYTATK
                     HAITGLTRSISLDGRAYDIACSQIDIGNAATEMTERMTGGVLQATGETKVEPRMDVAH
                     VASTVVHMASLPLDANIQFVTVMATKMPFIGRG"
     gene            3758..4759
                     /locus_tag="P5558_17015"
     CDS             3758..4759
                     /locus_tag="P5558_17015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HRY26072.1"
                     /translation="MTSPPLLPRVEGLYCPAGDFFIDPVRPVERALVTHGHADHARAG
                     HRAVLATAETLDIMAIRYGPDFARETQAADSAWITLGESRVRFVPAGHILGSAQIVVE
                     VGGTRVVAGGDFKRGSDPTCAEFELVPCDVFISEATFGLPVFRHPPARDEIGKLIASL
                     GVNVHRTHLVGAYALGKAQRVIRLLRDAGYDAPIFLHGSLQRLCDYYQSRGVALGRLL
                     PATVDAPRDGFAGRVVIGPPTSFGTPWARRFADPMIAFASGWMGVRPRIRQRGVELPL
                     VISDHSDWPELTQTIEDVGAEQIWVTHGQEEALVRWCELQGIRARPLNLVGYEEEPE"
     gene            4756..6345
                     /locus_tag="P5558_17020"
     CDS             4756..6345
                     /locus_tag="P5558_17020"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HRY26073.1"
                     /translation="MKAFARLLDQLILTPSRNEKVALVRRYLGTTPDPDRGYALAALT
                     GDLHLPAVKPAVLRALVAERVDEVLFRLSYDYVGDLAETISLIWEAAGASEDLPLSVV
                     VERLTSASRGAAAAVVGDLLDRLDPSSRYALLKLVTGNLRTGFSARLAKVALAEHGGV
                     PVTEIEELWHGLVAPYGELFAWLDGRASKPVNLARGPFRPVMLATPVSESELAALDPA
                     AFAAEWKWDGIRVQVVSDRGVRRLYSRSGDDISAAFPDALDAIEFEGSIDGELLVRHE
                     GGVAPFAALQQRLNRKTVGKATLADYPAFIRAYDLLVDADDDLRHLPFRDRRARLESI
                     ITGKSPDRLDLSPLIPFEAWDELATRREAPPSPVIEGVMLKRWDSAYLAGRPKGPWFK
                     WKRDPFVIDAVLMYAQRGHGRRSSFYSDYTFGVWTGEGALTPVGKAYFGFTDEELLQI
                     DRYVRDNTVERFGPVRAVRAEPGHGLVLEVAFEGLNRSRRHKSGVAMRFPRISRLRWD
                     KRPAEADRLEALESLLETTGA"
     gene            complement(6351..6983)
                     /locus_tag="P5558_17025"
     CDS             complement(6351..6983)
                     /locus_tag="P5558_17025"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HRY26074.1"
                     /translation="MSESRLRESICRFGQSIFERGLTFGSSGNISVRLDDGWLMTPTG
                     ATLGTLDPARISRLDADGRHVSGDQPTKETFLHLGMYANRVGARAVVHLHSTHSVAVS
                     CLADIDPADVIPPITAYYVMRVGTLPLVPYYRPGDPALGPAVEKLAGRHHAVLLANHG
                     PVVAGTSLENAVFATEELEETAKLWLMLRHEKIRALTPEQVADLKAHFPS"
     gene            complement(6980..8260)
                     /locus_tag="P5558_17030"
     CDS             complement(6980..8260)
                     /locus_tag="P5558_17030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRY26075.1"
                     /translation="MGILLGCIADDFTGATDLCNTLVKEGMRTVQLIGVPGPGLPVPE
                     ADAVTIALKSRTAPVADAVDESLEALRWLQSAGATQILFKYCSTFDSTPDGNIGPVAD
                     ALMEALGAGIAVVCPAFPETGRTIYLGHLFVGAKLLSDTHMRHHPLTPMTDSDLVRVM
                     GAQSKRPVGLVPFAAVDQGAGAIAERFAELEAAGIAYAVTDAVADRHLRHLGEAMADH
                     KLITGGSGIALGLPENFRRRGRLGQHDAAALPAVDGFEAVLAGSCSTATLEQIDRMAD
                     RNPALKLDPFALADGYAAELAIDWAKEHLANGPVLIYASAPADDVAEAQKVLGRERAG
                     SLIEDAMAAIARGLVEAGVRRLVVAGGETSGAVVKALGIEGLAIGPQIDPGVPACTSL
                     GKTPLALALKSGNFGGPDFLTRAFAHMPGERRRP"
     gene            complement(8291..8950)
                     /locus_tag="P5558_17035"
     CDS             complement(8291..8950)
                     /locus_tag="P5558_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY26076.1"
                     /translation="MPDQRTSPGPGPGPDCPDCPRLVAFRLAARTREPDWHNAPVPSF
                     GDPKARLLIVGLAPGLGGANRTGRPFTGDGAGFTLYPALARFGWTRGTFDARVDDGLE
                     LIDCRITNAVRCVPPQNKPTGAEIATCRRFLQTEILAPPRPRAILALGRIAHDSTLRA
                     LGLRLAAYPFGHGAMHEIGPDLVLASSYHCSRYNMNTGRLTETMLDHVLLALRRHLDA
                     R"
     gene            complement(8991..9515)
                     /locus_tag="P5558_17040"
     CDS             complement(8991..9515)
                     /locus_tag="P5558_17040"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRY26077.1"
                     /translation="MTSHPSHIAIGENEYVETLGLELDAIEPGLVIEHRPGFAFSLAE
                     ARYRSALAGDHAPVAVDPAFAALAGGGEAAIAQTWVLTAVVAATTRAFGRVVANLAWE
                     NVRFPGPVRDGDFVLVTSTILGKRNSASRPEQGILHVRTDAVTRGGTEVCSFERRLLV
                     YRGASAPHRAAGYA"
     gene            complement(9530..>10182)
                     /locus_tag="P5558_17045"
     CDS             complement(9530..>10182)
                     /locus_tag="P5558_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRY26078.1"
                     /translation="PRRARPPAPAPPAATPSASLPRPFALAFSPLARPSSSQSSRWST
                     PLFEAFHEIAPGRIRELTGLPFEAFAAGQVFHHRPGITVSQAENADEALATLNQAALH
                     YDEHYAGATEFGRPLVVSTLTLQRAVGMGWKTFGRRRAILGFASIRLTSPVLGGDTLY
                     AATRILAVADDLADPDCGIVTGEATVTRADGTTVAEVTYDQSIYRHDSGPFAALDY"
BASE COUNT         1551 a         3158 c         3964 g         1509 t
ORIGIN      
        1 tgcggccctg gtagaagccc atgggccgct cgccggtgag ccgggcctgg atcgcgacgg
       61 ccatccggat gtgctcgcgc tccacctcct ccgggacgtg ctggtagtcg atccagcgat
      121 agccgtggct ggcgatctcc cagcctgcct cgaccatggc ggcggcggcc tcggggttgc
      181 gctcgagggc catggcgacg gcgaagacgg tgaccggcag gcggcgctcg gtgaacaggc
      241 ggcgcaggcg ccagaagccg gcgcggctgc cgtattcgta gatcgattcc atgttcatgt
      301 ggcgcacgcc cggcagggcc tgggcaccga cgatctcgga gaggaaggcc tcgcaggccg
      361 ggtcgccgtg gaggatgcag ttctcgccgc cctcctcgta attgatgacg aactggacgg
      421 cgatccgcgc cccgttcggc cattgcgggt ggggggtgtg ctggccgtag ccccgcatgt
      481 cgcgggggta gggggagtcg ggcatggctt ctggtcccgg ctgctggcgc ctcgctcgtc
      541 gtccggcggc ttctagagca ttttcacggt aacggtatac ttcgtcaggc tgcggacggc
      601 cggtgctcac gtaggtagac tgcgctgcgc gccgatcgcc ttgccttcct cgtctacggc
      661 caccgtggaa acgctctaac agttgggaac ggcgagggcg aggggcagcg ggcgggtgtg
      721 gggggccggc tcagaagacg gcgcctcgat gggccttggg ggcggcgtgg attcggggtg
      781 cctggctggg gcggcggaag ccgccggcga agaggcggtt gagccaccgg cgggccggcc
      841 gctcgaccag gtggtgggcg agcatcgccg cgaggagggt gagggcgagc gcgatcgggc
      901 cgagccacca gaaatgcggc gaggcgggat cccggagcag caggccgagg ccgcggaaga
      961 agacgatgaa gacgatcgcg tgggtcatgt agatcgcata ggagatctcg ccgagataga
     1021 cggggacggg ccctgagagc caggcgaggc ggccctgccg ggcgaggctc gccacggcga
     1081 agatcaggaa ggccaggagg acgacggccc agaggccgtc gaggcgccag tggacgatcg
     1141 cgaggatgcc gccgagcgtg accgcgagga tggcgccggc gtggcgggcc tcgaggtccc
     1201 aggtgcggat ggcatggtag agggtgatgc cgaggacgaa ctccggcagg atgcgcagga
     1261 tgccgaaatc gtgggtgagg tgggtgagcg gccggccgat cgcctgctcg gcgacgaatg
     1321 aggcgacgag gaggccgagc ccggcgagga cgagcctgcc gcccgggccg aagggcaggc
     1381 ggagcacgag caggctgagc ggcaggaaga ggagataggc gaaccactcg gcgctgatcg
     1441 accaggagac gtagttccag ctcatgtggt cctggatgcc ccaggcgtgc agcatgagga
     1501 ggttcggcca gacggcctcc agcgtgtagg cctcgggcac gctgaagccg atgccggcga
     1561 gccggccggc cgtgacatag gcgacgacca cgaagaacgt ggccagatga aggggataga
     1621 ggcgggcgaa gcggcgcagc atgaagccct gccaggcgaa gtccccctgc tcgagccggc
     1681 cgagatagac gtgggtcagg atgaagccgg agagcacgaa gaagaagtcg acgccgagct
     1741 tgccctcggc gaagaagccg gtcaccgctg cgaagtcggc gggcatgaag tcggtgtagt
     1801 gaagggccag gacccagaag gcggcgaaga agcgcaaggc cgtcagcgcc cgcacgtcgt
     1861 ccggcagcgg ggcgatggcg ggcttgaaca agggcatggg cacgagctcg ttccatccgg
     1921 gcgagttggt gccgaccacc cgggtcgggc gcatcgacag acgagacccg catcctgcgc
     1981 caaggacgtt aacggccgtt taacgttcgt ccttactaac ggatctcaac catgtttcgg
     2041 cgtaccctct tgctcatccg ggtggcttgc cgcaaaccgg ggccgcaacg gcaaccggtg
     2101 atgcgcgatc ggggccgtcc agggaggaag aagccgtgcc gcgttttgcc gccaatctca
     2161 gcatgatgtt caccgaccgg ccgtttctcg accgtttcgc cgcggccggg gcggccgggt
     2221 tcgccgcggt cgagtatctc ttcccctacg agcacgaggc gggcgagatc cgggcggcgc
     2281 tggatgacgc cgggctcgag caggcgctct tcaacctgcc gcccggcgat ttcgccaggg
     2341 gcgagcgcgg gctcgcggcc ctgccggggc gcgaggcgga gttcagggac ggcctcgaca
     2401 aggcgctgca ctacgccgag gtcatcgggg cgaggcggct gcactgcatg gccgggctga
     2461 tcggcgccga cgacgaccgg gcgcattttt ccagcaccta tctcgagaac ctggcgctgg
     2521 cggcggagcg ggccgccgcc aagggcatca ccatgctgat cgagccgatc aacacgcgct
     2581 cgatcccggg ctacttcctc aactaccagc gcgatggccg gcggatcatc gagcgggtgg
     2641 gggcgccgaa cctgaagctg cagttcgact gcttccactg ccagatcatg gagggcgacc
     2701 tcgccatgca tcttcgggag ctgatcggcg tgattggcca tatccagatc gcgggcgtgc
     2761 cggaccggca cgagccggac acgggcgagg tcgcctatcc ctacctcttc ggcgtgatgg
     2821 accagatcgg ctacgccggc tgggtgggct gcgagtacaa cccgaagggg cggaccgagg
     2881 acgggctcgg ctggtttggg ccctaccgca ggaattgaga cgaacctatt caacggatgg
     2941 agcgaatgac aatgacgaag gtcgcaatgg tgacgggcgc cggcagcggc atcggcaagg
     3001 cttcggcgct cggtctcgcc ggggccggct acaagctggt gctggccggc cggcggcagg
     3061 agcccctggc ggcggtggcg gccgagatcg agggcacggg cggccaggcg ctggcggtct
     3121 cgaccgatgt gggcgatgcg gagtccgtgg ccgccctctt cgaggcctgc gccgagcgct
     3181 tcggccggct cgacgtgctc ttcaacaatg ccggggtggg ctcgccgccg gtgccgatcg
     3241 acgagctgcc gctcgagcag tggaggaagg tggtggacgc caacctcaac ggcgccttct
     3301 actgctcgcg cgaggcgttc ggcatgatgc gcaagcagaa gccgcagggc gggcggatca
     3361 tcaacaacgg ctccatctcg gcgcatgtgc cgcggccgtt ctcggcgccc tacacggcca
     3421 ccaagcacgc gatcaccggg ctcacccggt cgatcagcct cgacggccgg gcctacgaca
     3481 tcgcctgcag ccagatcgac atcggcaatg ccgcgaccga gatgaccgag cggatgaccg
     3541 gcggcgtgct gcaggcgaca ggcgagacca aggtcgagcc gcgcatggac gtggcgcatg
     3601 tggcgagcac ggtggtccac atggcgagcc tgccgctcga tgccaacatc cagttcgtga
     3661 cggtgatggc caccaagatg ccgttcatcg ggcgggggta gcgaacgtcg gacggggctc
     3721 tcgcggtcgt ccggccgtgt agggcgaggc cgcctcgatg acgtcaccgc ccctcctgcc
     3781 gagggtcgag ggcctctatt gcccggcggg cgatttcttc atcgacccgg tcaggccggt
     3841 cgagcgcgcg ctcgtcacgc atggccatgc cgaccatgcg cgggcggggc atcgggccgt
     3901 tctggcgacg gcggaaacgc tcgacatcat ggcgatccgc tatgggccgg actttgccag
     3961 ggaaacgcag gccgcggaca gtgcgtggat cacgctcggc gagagccgcg tgcgcttcgt
     4021 cccggcgggg catatcctgg gctcggcgca gatcgtggtg gaggttggtg gcacaagggt
     4081 cgtcgcgggc ggggatttca agcggggcag cgatccgacc tgcgccgagt tcgagctcgt
     4141 cccttgtgac gtcttcatca gcgaggcgac cttcggcctg ccggtctttc gccatccacc
     4201 ggcccgcgac gaaatcggca agctcatcgc ctcgctgggc gtgaatgtcc accggacgca
     4261 tctggtcggc gcctatgcgc tggggaaggc gcagcgggtc atccggctcc tgcgtgacgc
     4321 gggctacgac gcgccgatct tcctgcacgg ctcgctgcag cgtctgtgcg attattatca
     4381 atcgcggggc gtggcgctcg gcaggctgct gccggcgacc gtcgatgcgc cgcgggatgg
     4441 cttcgcgggc cgggtggtga tcgggccgcc caccagcttc ggcacgccct gggccaggcg
     4501 cttcgccgat cccatgatcg ccttcgcctc cggctggatg ggggttcgcc cccgcatccg
     4561 gcaacgcggc gtggagctgc cgctggtcat ctccgaccat tccgactggc cggagctgac
     4621 gcagacgatc gaggatgtcg gggcggaaca gatctgggtg acgcatggcc aggaagaggc
     4681 gctggtgcgg tggtgcgagc tgcagggcat cagggcgcga ccgctgaacc tcgtgggcta
     4741 cgaggaagag ccggaatgaa ggcctttgcc cggcttctgg accagttgat cctcacgccg
     4801 tcgcgcaacg agaaggtggc cctcgtccgg cgctatctcg gcacgacgcc ggacccggat
     4861 cggggctacg cgctggcggc gctgacgggt gatctccatc tgccggcggt caagcccgcg
     4921 gtgctgcggg cactggtggc ggagcgggtg gacgaggtgc tgttccggct ctcctacgac
     4981 tatgtgggcg atctcgccga gaccatctcg ctgatctggg aggcggcggg ggcaagcgag
     5041 gacctgccct tgtcggttgt cgtggaacgg ctgacctcgg cctcccgggg cgctgcggcg
     5101 gcggtcgtcg gcgatctcct cgaccggctc gacccctcgt cgcgctatgc gctcttgaag
     5161 ctcgtgacgg gcaatctcag gaccgggttc tccgcgcgtc tggccaaggt ggccctggcc
     5221 gagcacggcg gcgtgccggt caccgagatc gaggagctct ggcatgggct cgtcgcgcct
     5281 tacggcgagc tcttcgcctg gctggacggg cgggcgtcga agcccgtgaa cctggcacgc
     5341 gggccctttc gccccgtgat gctggcgacg ccggtgagcg agagcgagct ggcagcgctc
     5401 gaccctgcgg cgtttgcggc agagtggaaa tgggacggga ttcgggtgca ggtggtctcg
     5461 gaccggggcg tgcggcggct gtattcccgc agcggagacg acatttccgc cgcgtttccc
     5521 gatgcgctgg acgcgatcga attcgaaggc tcgatcgatg gggagctgct ggtgcggcac
     5581 gagggcgggg tcgcaccgtt cgcggccctg cagcagcgcc tcaaccgcaa gaccgtcggc
     5641 aaggcgacgc tggcagacta ccccgccttc atccgcgcct atgatctgct cgtcgacgcg
     5701 gacgacgatc tgcgccacct gccgtttcgc gatcgccgcg cgcggctgga aagcatcatt
     5761 acgggaaaga gcccggaccg tctcgatctc tcgccgctga tccccttcga ggcgtgggac
     5821 gagctcgcca cccggcgcga ggcaccaccc agcccggtga tcgagggtgt catgctgaag
     5881 cggtgggaca gcgcctatct cgccgggcgg ccgaaaggcc cgtggttcaa gtggaagcgc
     5941 gatcccttcg tcatcgacgc cgttctcatg tacgcgcagc gcgggcacgg gcggcggtcg
     6001 agcttctatt cagactacac gttcggcgtc tggaccgggg agggcgccct gacgccggtc
     6061 ggcaaggcct atttcgggtt cacggacgag gagctgctcc agatcgatcg ctacgtgcga
     6121 gacaacacgg tggagaggtt cggcccggtg cgggcggtca gggcggagcc ggggcatggt
     6181 ctcgtgctcg aggtcgcctt cgaggggctg aaccgttcga ggcggcacaa gtccggcgtg
     6241 gcgatgcgct ttccccgcat ctcgcggctc agatgggaca agcggccggc cgaggccgac
     6301 cggctcgagg ccctcgaatc gctgctcgag acgaccggcg cctgataagc tcagctaggg
     6361 aaatgcgcct tgaggtcggc gacctgctcc ggggtcaggg cgcggatctt ctcgtggcgg
     6421 agcatcagcc agagcttcgc ggtttcctcc agctcctcgg tggcgaagac ggcgttctcg
     6481 aggctggtgc cggcgacgac ggggccgtga ttggcgagca gcacggcgtg gtgcctgccg
     6541 gcgagctttt ccacggccgg gccgagggcc gggtcgcccg ggcggtagta ggggacgagc
     6601 ggcagggtgc cgacgcgcat cacgtagtag gcggtgatcg gcgggatgac gtcggcggga
     6661 tcgatgtcgg cgaggcagct cacggcaacc gaatgggtcg agtggagatg cacgacggcc
     6721 cgcgcgccaa cacggtttgc gtacatgccg agatggagga aggtctcctt ggtcggctgg
     6781 tcgcccgaga cgtggcggcc gtcggcatcg agcctggaga tgcgggccgg gtcgagcgtg
     6841 ccgagcgtgg cgccggtggg tgtcatgagc cagccatcgt cgaggcggac ggagatgttg
     6901 ccggagctgc cgaaggtgag gccgcgctcg aagatcgatt ggccgaagcg gcagatggac
     6961 tcgcggagcc ggctttcgct catggccgcc gccgttcccc tggcatgtgg gcgaaggccc
     7021 gggtgaggaa gtcggggccg ccgaaattgc cggacttgag ggcgagggcg aggggcgtct
     7081 tgccgaggct ggtgcaggcg gggacacccg ggtcgatctg cgggccgatg gcgaggcctt
     7141 cgatgccgag cgccttgacc acggcccccg aggtctcgcc gccggcgacg acgaggcggc
     7201 ggacgcccgc ctcgaccagg cccctggcga tggccgccat ggcgtcctcg atcaggctgc
     7261 cggcccgctc gcggccgagc accttctgcg cctcggcgac gtcgtcggcg ggcgcgctgg
     7321 cgtagatcag gaccgggccg ttcgccaggt gctccttcgc ccagtcgatg gcgagctcgg
     7381 cggcatagcc gtcggcgagg gcgaaggggt cgagcttcag cgccgggttg cggtcggcca
     7441 tgcggtcgat ctgctccagg gtcgcggtgg agcaggagcc ggcgagcacg gcctcgaagc
     7501 cgtccacggc cggcagggcc gccgcgtcgt gctggccgag gcggccgcgg cggcggaaat
     7561 tctcgggcag gcccagggcg atgccggagc cgccggtgat cagcttgtgg tcggccatgg
     7621 cctcgcccag gtggcgcaga tgccgatcgg ccacggcatc ggtgacggca taggcgatgc
     7681 cggccgcctc gagctcggcg aagcgctcgg cgatggcccc cgcaccctgg tcgacggcgg
     7741 cgaagggaac gagccccacg ggccttttgg actgggcccc catgacccgg acgaggtcgc
     7801 tgtcggtcat cggggtcagc ggatggtggc gcatgtgggt gtcgctgagc agcttcgcgc
     7861 cgacgaagag gtggccgagg tagatggtcc ggccggtctc ggggaaggcc gggcagacga
     7921 cggcgatgcc ggcgccgagg gcttccatga gcgcgtcggc gaccgggccg atattgccgt
     7981 cgggcgtgga atcgaaggtg gagcagtact tgaagaggat ctgggtggca cccgccgact
     8041 ggagccagcg gagggcttcg agcgattcgt ccacggcgtc ggcgacggga gcggtgcgcg
     8101 acttcagcgc gatggtgacg gcgtcggcct cgggcaccgg caggccgggc ccgggcacgc
     8161 cgatgagctg gaccgtgcgc atgccctcct tgaccagcgt gttgcagagg tcggtggcgc
     8221 cggtgaagtc gtcggcgatg cagccgagca ggatgcccaa ggtcgtctcc ccagaattgt
     8281 tggccgaacc ttaccgagcg tcgagatggc ggcgcaaggc cagcagcaca tggtcgagca
     8341 tggtctcggt gagccgaccg gtgttcatgt tgtaacggga gcagtggtag ctcgaggcga
     8401 gcacgaggtc cggcccaatc tcatgcatcg caccgtggcc gaagggatag gccgccaggc
     8461 gaaggcccag ggcgcgcagt gtcgagtcgt gggcgatgcg gccgagcgcc aggatcgccc
     8521 gggggcgggg tggcgccagg atctccgttt gcaggaaccg gcggcaggtg gcgatctcgg
     8581 ccccggtcgg cttgttctgt ggcggcacgc agcgcacggc gttggtgatg cggcagtcga
     8641 tcagctcgag cccgtcgtcg acgcgggcat cgaacgtgcc ccgggtccag ccgaagcggg
     8701 cgagggcggg atagagcgtg aagccggcgc cgtcgccggt gaaggggcgg ccggtgcggt
     8761 tggcgccgcc gaggccgggg gcgaggccga cgatcaggag gcgcgccttc gggtcaccga
     8821 aggaggggac gggcgcgttg tgccagtccg gctccctggt gcgcgcggcg aggcggaagg
     8881 cgaccagccg cgggcagtcg gggcagtcgg ggccggggcc ggggccgggg gacgtgcgct
     8941 ggtctggcat gcccgcctca taagcgagtg gtgtacgtac gccaagccgc tcaggcgtag
     9001 ccggctgccc gatggggggc gctggcgccg cgatagacga ggagccggcg ctcgaagctg
     9061 cagacctcgg tgccgccgcg ggtcacggca tcggtcctga catgcaggat gccctgctcg
     9121 ggccgggagg cggagttgcg cttgcccagg atcgtcgagg tgacgagcac gaaatcgccg
     9181 tctcggaccg ggccggggaa gcggacgttc tcccaggcga ggttcgccac gacgcggccg
     9241 aaggcgcggg tcgtggcggc gacgacggcg gtgagcaccc aggtttgcgc gatggccgcc
     9301 tcgccgccgc cggccagcgc ggcgaaggcg gggtccaccg ccaccggcgc gtggtcgccg
     9361 gcgagggcgg atcggtagcg tgcctcggcg aggctgaagg cgaagccggg gcggtgctcg
     9421 atcacgaggc cgggctcgat ggcgtcgagc tcgaggccga gggtctcgac gtactcgttc
     9481 tcgccgatgg cgatgtgcga cgggtgcgag gtcatcgggc ggcgctcctt cagtagtcga
     9541 gggcggcgaa gggaccgctg tcatggcgat agatcgactg gtcgtaggtc acctcggcaa
     9601 cggtggtccc gtcggcgcgg gtgacggtcg cctcgcccgt cacgatgccg caatcgggat
     9661 cggccaggtc gtcggcgacg gcgaggattc tggtcgccgc atagagcgtg tcaccgccga
     9721 gcaccgggct cgtgagccgg atcgaggcga agccgagaat cgcccgccgg cggccgaagg
     9781 tcttccagcc catgccgacg gcgcgctgca gggtcagcgt gctcaccacg agcggccggc
     9841 cgaactcggt agcaccggca tagtgctcgt cataatgcag ggccgcctgg ttcagcgtgg
     9901 cgagcgcctc gtcggcattc tccgcctggc tcaccgtgat gcccgggcgg tggtggaaga
     9961 cctgcccggc ggcgaaggcc tcgaacggca ggccggtgag ctcgcggatg cgcccggggg
    10021 cgatctcgtg gaaggcttcg aacagcggcg tgctccagcg cgaggattgg cttgagcttg
    10081 gccgggcgag cggggaaaag gcaagggcga agggtcgggg aagggaggcg gatggggttg
    10141 ctgcaggggg tgcgggtgct ggcggtcgag ctctacgggg cc
//
LOCUS       DAOTFI010000183        10122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7901_length_10122_cov_6.812854, whole genome shotgun sequence.
ACCESSION   DAOTFI010000183 DAOTFI010000000
VERSION     DAOTFI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7901_length_10122_cov_6.812854"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="P5558_17050"
     CDS             complement(<1..1628)
                     /locus_tag="P5558_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY26079.1"
                     /translation="MQQLEGVARGDQNRALTMSTIAFTACFAVWTIFSIIGIQIKQEL
                     GLSETQFGLLVGLPILTGSLSRIFLGIWTDQYGGRIVYVAVMVLAAIATLLLTFADTY
                     VMTLVAALGVGLAGGSFAVGIAYVSRWYPTQKQGTALGIFGVGNVGAAVTKFCAPFVL
                     VAFGWQMVANVWAIGLLIMAAVFWFTTSDDPVLAARRRRGEKPRGTLLELTPLRNLQV
                     WRFALYYFFVFGGFVALALWLPHYLIGVYGLDIKSAGMIAAFYSVPASLFRAYGGHLS
                     DRYGARSVMYWTFGVSLLCLFMLAYPPTAYVIRGIEGPIAFSTEMGLAPFIVVIFVLG
                     FFMSLGKAAVYKHIPVYYPGHVGAVGGLVGMIGGLGGFILPIVFGVMNDLTGIWTSCF
                     ALLFAVVGVSLLWMHFAIRRMEQAAMGKELETLPQLPEFQEIHEPARHGTGTGKVLTD
                     WRPEDAEFWAREGKGIANRNLWISIPCLLLAFSVWMVWSVVVAKLPAVGFEFTTSQLF
                     WLAALPGLSGATLRIFYSFMVPIFGGRMWTTLTTA"
     gene            1895..2581
                     /locus_tag="P5558_17055"
     CDS             1895..2581
                     /locus_tag="P5558_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY26080.1"
                     /translation="MRPEEADEIRTLPLFRDCADAHFTTLLERGFLQRFPPGLVLIHE
                     DDPADFLHVVVEGMVEMVTSHAGRETTLGFVRPFGTFILAAVLTDQVYLQAARTLEKS
                     RILMVPAAAVRDTMRADPAFMTAVTRELATAYRDTVMELKSQKLRNGAERLANWLLRH
                     ERDGAVLIPVEKRTLAARLGMTPENLSRAFATLGACNIEVQGPKVLLHDRGALEKLAK
                     PSPLIDDVST"
     gene            complement(2592..3101)
                     /locus_tag="P5558_17060"
     CDS             complement(2592..3101)
                     /locus_tag="P5558_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY26081.1"
                     /translation="MITTDYVRTMARYNAWQNGLVYAAADTLDEPARRAERGTFFGSI
                     HKTLCHLLWGDQIWLHRFAGTPPPAVPDIPRSVALCATWEELRAERRRFDDVIRDWAA
                     GLDPDWIEGELSWYSGAIGREVTRPRALLVAHLFNHQTHHRGQVHAMLTAAGAKTGPT
                     DLPFMPEES"
     gene            3182..4036
                     /locus_tag="P5558_17065"
     CDS             3182..4036
                     /locus_tag="P5558_17065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY26082.1"
                     /translation="MVRFGMHSSLWTAAWTRAAAEQVVPLAAGHGLDVIEIALLEPAA
                     VDVPHSRALFERHGIAPTCSLGLPFEVCAPLHPEKATAFLMTALEAAHGLGSNTLSGV
                     TFSTLGYTTGVPPTEGEYANIAEALGPVARRAGELGMTLGLEPCNRYETHLVNTADQA
                     VALIERIGEPALMIHLDTYHMNIEEKGFRSGVLKAGRHLKYIHLSESDRGVPGTGTVD
                     FAATMAALAEIGFQGDIVGEAFINMPPALAKALSVWRPVAKSAEEVLDPGMTMLKRLA
                     VEHGLVAA"
     gene            4044..4931
                     /locus_tag="P5558_17070"
     CDS             4044..4931
                     /locus_tag="P5558_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26083.1"
                     /translation="MDPEQRQVLIALGLVIAGGVLFVIMNTLVKFLTTELNPVMLTWA
                     RYFFHVAFVVVVFPKSLLGVLRADRIPFQIGRSVLLLLATITNFIALALLPLAEVASI
                     IFLAPIFVAGLAVLVLRERVVAWRWLLIGLGFSGALIIVQPQGGTFGLGAAAALACAF
                     FYALYQITTRIVREAQPIVSLLYGGLVGIVALSLVVPFYWQAPTVTQWLLFVSIGSLG
                     AAGHLLVIMALQRAEASKMAPFTYVQLLWAMIASYVVFADLPSASTVIGAAVIVTSGL
                     LLYRLDLAEAASRSAAKAA"
     gene            4928..5968
                     /locus_tag="P5558_17075"
     CDS             4928..5968
                     /locus_tag="P5558_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRY26084.1"
                     /translation="MSNVASPPAPGFDLGRLDPFLRGRLPGLQGEPRIERIAGGQSNP
                     TFFVTFANRRLVLRKKPDGPILPSAHAVDREYRVLTALGATGVPVPRTILYSDDEEII
                     GTPFYLMERLDGRVVHDNALPGFEPAERRAVYRSMAETLARLHAVDPAAVGLADFGRP
                     ANYFSRQIARWTRQWELSKTRDLADIERLIDWLPAHLPPDDTAAIVHGDFRLGNLMLH
                     PAEPRVIGILDWELSTLGHPLADLAHAAMAWHSTPAEFGGLVGLDREALGIPDEAEFL
                     RAYEANGGHGLRLQPFHHAMALFRFAVIFEGIAARAQAGNASADNAALVGRLSARFAR
                     RAVEVIEGIVHV"
     gene            5984..7222
                     /locus_tag="P5558_17080"
     CDS             5984..7222
                     /locus_tag="P5558_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY26085.1"
                     /translation="MEFAFSAEVEALRDQLSAFMAEHVLPADPAFRAAASSGRHAVEI
                     LEPLKARAREAGLWNLFLPALRDDEPGTRLSNLAYAPLAEIMGRVPWSAEVFNCSAPD
                     TGNMELLHLCATPAQRERWLMPLLDGTIRSCVAITEPGVASSDPTNLKTTIRRDGDTF
                     VVNGRKWFTTGALHPACRFAILMGVTDERPDADPHARHSMVIVPLDTPGVEIVRNLPI
                     MGHLAPEGHCEVDFEDVRIPAANLLGEAGRGFALAQARLGPGRVHHCMRSIGQCELAL
                     ELMADRAMAREAFGRPLADFANIQDWIALSRMQIDQARLYVLRTAWLMDRDGNKAARA
                     EVSAIKLVVSRLQTEVLDRAIQVFGAMGLTDDTPLAHLWTWGRALRFVDGPDEVHLRT
                     VARAELRRARHRATGAGPGS"
     gene            7227..8813
                     /locus_tag="P5558_17085"
     CDS             7227..8813
                     /locus_tag="P5558_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY26086.1"
                     /translation="MRRDAHLDPRPANFAPLTPLDFLRRSIEVFPGKTAVVAGARRLS
                     YTDLGELVGRLAAGLRRSGIGRGDVVSVIAGNTPELLALHFAVPLTGAVLNAINTRLD
                     PATVAHILAHAEARLVMADAERAPLTRAALAQGGLETPLVTLDALSLAGFTEGLPPLP
                     VSLDAVADEWQPISLNYTSGTTGSPKGAVYHHRGAHLNALGNVMALGFDERSVYLWTL
                     PMFHCNGWCHPWAVTAVGGTHVCLDRPDPAAIVAALREEAVTHFACAPVVLYNLLHQG
                     REQSPRLPRRVRVATGGAAPTTALIEGLDAMGIELIHLYGLTESLGPVTACAERADWG
                     EPDAAEKARLLARQGVRHPLAAEVAVVDAAGEPVPMDGATMGEIVIRSNTLMAGYLKD
                     RAATEAAFRDGRLNTGDLAVRHPDGFIQIRDRAKDVIISGGENISSLEVEGVLHQHPA
                     VLLAAVVAVADEKWGEVPVACLELKPGQAAPPAAELEAFCRDRLAHFKVPRRFVLREL
                     PRTATGKIQKFQLRAELALD"
     gene            complement(8824..9765)
                     /locus_tag="P5558_17090"
     CDS             complement(8824..9765)
                     /locus_tag="P5558_17090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY26087.1"
                     /translation="MPRDQGAMPDTLNLLIVEDSDAERALLAAELASCPRLAPRMAAS
                     LHDAADAIATTPPDVVLLDLELPDARGLNGVEWLQRHHPALAVVVLSGFAADDLLVAT
                     EAIRQGAQDFLCKGRIDADRLYRTLKLAVERKAREHRLVQGAVRDPLTGLPWLPQLEE
                     RFGRAVARTARNRAHLALLHIAVDDFASLRETHGIDRAEAALVELAHRLGRQMRRTDA
                     VAKLDDTTFVALIDGMKHISDAYVVARKIIAAAREPILVGDATLRVRPSIGVVTGSGP
                     GLDFRALLARAEAAMDEARRQGGERYAAAAELVPALG"
     gene            complement(9833..>10122)
                     /locus_tag="P5558_17095"
     CDS             complement(9833..>10122)
                     /locus_tag="P5558_17095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY26088.1"
                     /translation="GIASGGRSRRQALAGGEIYVLYVHPDQHGAGIGRALFDACHFEL
                     ARRRYAGTLVWVLASNPARGFYEHLGGERVAENTLEIGGSRVLEVAYLWRD"
BASE COUNT         1531 a         3662 c         3378 g         1551 t
ORIGIN      
        1 gccgtggtca gcgtggtcca catccgcccg ccgaagatcg gcaccatgaa ggagtagaag
       61 atcctgagcg tggcgccgga gaggccgggg agggccgcga gccagaagag ctggctggtg
      121 gtgaactcga agccgaccgc cggcagcttg gcgacgacca cgctccagac catccagacc
      181 gagaaggcga ggagcaggca ggggatcgag atccacagat tgcggttggc gatgcccttg
      241 ccttccctgg cccagaactc ggcgtcctcg ggccgccagt cggtgagcac cttgcccgtg
      301 ccggtgccgt gccgcgccgg ctcgtgaatc tcctggaact ccggcagctg cggcagggtt
      361 tccagctcct tgcccatcgc cgcctgctcc atgcgacgga tggcgaagtg catccagagc
      421 agcgagacgc cgacgaccgc gaagaggagg gcgaagcagc tcgtccagat gccggtgaga
      481 tcgttcatga caccgaagac gatcggcagg atgaagccgc cgaggccgcc gatcatgccg
      541 acgaggccgc cgacggcgcc cacatggccc gggtagtaga ccgggatgtg cttgtagacc
      601 gccgccttgc cgaggctcat gaagaagccg aggacgaaga tgacgacgat gaacggcgcc
      661 aggcccatct cggtcgagaa cgcgatcgga ccctcgatgc cgcggatcac gtacgcggtc
      721 ggcgggtagg cgagcatgaa caggcagagc agcgagacgc cgaaggtcca gtacatcacc
      781 gagcgggcgc cgtagcggtc ggagagatgg ccgccatagg cgcggaacag cgacgccggc
      841 accgagtaga aggcggcgat catgccggcc gacttgatgt cgaggccgta cacgccgatc
      901 agatagtgcg gcagccagag cgcgagcgcc acgaagccgc cgaacacgaa gaaatagtag
      961 agggcgaagc gccagacctg cagattgcgg agcggcgtca gctcgagcag cgtgccgcgc
     1021 ggcttctcgc cccgccggcg ccgcgccgcc agcaccgggt cgtcggatgt cgtgaaccag
     1081 aagaccgccg ccatgatcag gaggccgatg gcccagacat tggccaccat ctgccagccg
     1141 aaggcgacga ggacgaacgg ggcgcagaac ttggtgaccg cggccccgac attgccgacg
     1201 ccgaagatgc cgagcgcggt gccctgcttc tgcgtcgggt accagcgcga cacgtaggcg
     1261 atgccgaccg cgaacgagcc accggccagc ccgacaccga gcgccgccac cagcgtcatg
     1321 acataggtgt cggcgaaggt cagcagcagg gtggcgatcg ccgcgagcac catgacggcg
     1381 acatagacga tgcgcccgcc atactggtcg gtccagatgc cgagaaagat gcggctcaag
     1441 gagcccgtga ggatcggcag gccgacgagc agcccgaact gggtctcgct caggcccagc
     1501 tcctgcttga tctggatgcc gatgatcgag aagatcgtcc agaccgcgaa gcaggccgtg
     1561 aacgcgatgg tgctcatcgt cagggcgcgg ttctgatcgc cgcgcgcaac gccctcgagt
     1621 tgctgcatcg tgccgccttc ggaatcgttg ttgtccgtac cggcttgccg ccggccgtgg
     1681 tcatttgccc ggccggccgg tccggttacg ttgattcaga tcaagcgatt ggggtctcga
     1741 ggccaaaata attcatcgga gcagtttctt tgtcttggcg aatgcctcaa tcggggtcgg
     1801 tctttattaa agagcgttgc gtggattgct ttttatcaat cgttgccgcg cgggcgggcc
     1861 gccgataagc tgcccctacc atcgaggggg aaacatgcgg cccgaggaag cagacgagat
     1921 ccgtaccctg ccgctgttcc gcgactgcgc cgacgcgcac ttcacgacgc ttctggagcg
     1981 cggcttcctg cagcgcttcc cgcccgggct cgtcctgatc cacgaggacg accccgccga
     2041 tttcctccat gtcgtcgtcg agggcatggt cgagatggtg acgtcccatg ccggccgcga
     2101 gaccacgctc ggcttcgtcc ggcccttcgg cacgttcatc ctcgccgccg tgctgacgga
     2161 ccaggtttat ctgcaggccg cccgcacgct cgagaaatcc cgcatcctga tggttccggc
     2221 ggcagcggtc cgcgacacca tgcgcgccga tccggccttc atgacggccg tcacccggga
     2281 gctcgctacc gcctatcgcg acacggtcat ggagctgaag agccagaagc tgcgcaacgg
     2341 cgccgagcgc ctcgccaact ggctcctgcg ccacgagcgc gatggggccg tgctgatccc
     2401 ggtcgagaag cgcacgctcg ccgcccggct cggcatgacc ccggagaacc tgtcccgcgc
     2461 cttcgccacg ctgggcgcct gcaacatcga ggtgcagggc ccgaaggttc tcctccacga
     2521 ccgcggcgcc ctcgagaagc tggccaagcc gagcccgctg atcgacgacg tatcgacctg
     2581 agcggcccgg ctcaggactc ttccggcatg aagggcaggt cggtcgggcc ggtcttcgcc
     2641 ccggccgcgg tcagcatggc gtgcacctgg cctcgatggt gcgtctggtg attgaagaga
     2701 tgggcgacga gaagtgcgcg cgggcgggtc acctcgcgac cgatcgcccc agagtaccag
     2761 gagagctcgc cctcgatcca gtccggatcg agccccgccg cccagtcccg gatcacgtcg
     2821 tcgaagcgcc ggcgctcggc ccgcagctcc tcccaggtgg cgcagagggc cacggagcgc
     2881 ggaatgtcgg gcacggccgg cggcggggtg ccggcgaagc ggtgcagcca gatctggtcg
     2941 ccccagagga gatggcagag ggtcttgtgg atcgagccga agaaggtgcc ccgctcggcc
     3001 cgccgcgcag gctcgtcgag cgtgtcggcg gcagcgtaga ccagcccgtt ctgccaggca
     3061 ttgtagcggg ccatggtgcg cacgtagtcg gtcgtgatca tcgccgtcct ccccctttcg
     3121 cgggtcgcgg aacgcttcta cgttccggcg agcagtgaac agtcgtcccg ggagagacgc
     3181 aatggttcga ttcggcatgc acagcagcct ctggacggcg gcctggacga gggcggccgc
     3241 ggagcaggtc gtgccgctgg ccgccggcca cggcctcgac gtgatcgaga tcgccctgct
     3301 cgagcctgcc gccgtcgacg tgccgcacag ccgggcgctg ttcgagcggc acggcatagc
     3361 acccacctgc tcgctcggcc tgccgttcga ggtctgcgcc ccgctccatc cggagaaggc
     3421 gacggcattc ctcatgacgg cgctcgaggc ggcgcacggg ctggggtcca acacgctctc
     3481 cggcgtcacc ttctcgaccc tcggctacac cacgggcgtg ccgccgaccg aaggggagta
     3541 cgccaacatc gccgaggcgc tcgggccggt ggcgaggcgg gccggcgagc tcggcatgac
     3601 cctcggcctc gagccctgca accgctacga gacgcacctc gtcaacaccg ccgaccaggc
     3661 ggtggcgctg atcgagcgga tcggcgagcc ggcgctgatg atccatctcg acacctatca
     3721 catgaacatc gaggagaagg gctttcggag cggtgtcctg aaggcgggcc ggcacctgaa
     3781 gtacatccac ctctccgaga gcgaccgggg cgtgccgggc accggcacgg tggacttcgc
     3841 cgccaccatg gcggctctcg ccgagatcgg ctttcagggc gacatcgtcg gcgaggcgtt
     3901 catcaacatg ccgccggccc tcgccaaggc gctctccgtc tggcgcccgg tcgcgaagag
     3961 tgccgaggag gtgctcgatc ccggcatgac catgctgaag cggctcgcgg tcgagcacgg
     4021 cctggtggcg gcctgacggc atcgtggatc cggagcagcg acaggtcctg atcgccctcg
     4081 gcctggtcat cgccggcggc gtgctcttcg tcatcatgaa cacgctggtc aaattcctga
     4141 cgaccgagct caacccggtg atgctcacct gggcccgcta tttctttcat gtcgccttcg
     4201 tcgtcgtcgt cttcccgaaa agcctcctcg gcgtgctgcg cgccgaccgg atcccgttcc
     4261 agatcggccg ctcggtgctt ctcctgctgg cgaccatcac caatttcatc gccctggcgc
     4321 tcctgccgct cgccgaggtg gcgtcgatca tcttcctcgc ccccatcttc gtcgccggcc
     4381 tcgccgtgct ggtgctgcgc gagcgggtcg tcgcctggcg ctggctcctg atcggcctcg
     4441 gcttcagcgg tgcgctgatc atcgtccagc cgcagggcgg cacgttcggg ctgggtgccg
     4501 ccgcggccct cgcctgcgcc ttcttctatg ccctctacca gatcaccacg cggatcgtgc
     4561 gcgaggccca gcccatcgtc tcgctgctct atggcgggct ggtcggcatc gtcgccctca
     4621 gcctcgtggt gcccttctac tggcaggcgc cgaccgtcac ccagtggctg ctcttcgttt
     4681 ccatcggctc gctgggggcc gcgggccatc ttctcgtcat catggcgctg cagcgcgccg
     4741 aggcctcgaa gatggccccc ttcacctatg tccagctcct ctgggcgatg atcgcgagct
     4801 atgtcgtctt cgccgacctg ccgagcgctt caaccgtcat cggcgccgcc gtcatcgtca
     4861 ccagcggcct cctcctctac cgcctcgacc tggccgaggc ggcttcccgc tcggcggcga
     4921 aggcggcatg agcaacgtcg catcgccgcc ggcgccgggc ttcgacctcg gtcggctcga
     4981 ccccttcctg cgcggccgcc tgcccgggct ccagggcgag ccgcggatcg agaggatcgc
     5041 cggcggccag tccaacccga ccttcttcgt caccttcgcc aaccgccggc tggtgctgcg
     5101 caagaagccg gacggtccga tcctgccctc ggcccacgcc gtcgaccgcg agtaccgcgt
     5161 gctgacagct ctgggcgcga ccggcgtgcc ggtgccgcgg acgatcctgt attctgacga
     5221 cgaagagatc atcggcacgc ccttctacct gatggagcgg ctcgacggcc gggtggtgca
     5281 cgacaacgcg ctgcccggct tcgagccggc cgagcgccgc gccgtctacc gctccatggc
     5341 ggagaccctg gcccgtctgc acgcggtcga cccggccgcg gtgggcctcg ccgatttcgg
     5401 ccggccggcc aactatttca gccgccagat cgcccgctgg acgcgccagt gggagctgtc
     5461 caagacccgc gacctcgccg atatcgagcg gctgatcgac tggctgcccg cgcacctccc
     5521 gcctgacgac acggcggcca tcgtccacgg cgacttccgc ctcggcaacc tcatgctgca
     5581 tccggccgag ccccgcgtta tcggcatcct cgactgggag ctctcgaccc tgggccatcc
     5641 gctcgccgac ctcgcgcacg ccgccatggc ctggcactcg accccggccg agttcggcgg
     5701 gctcgtcggc ctcgatcgcg aggccctcgg catccccgac gaggcggagt tcctcagagc
     5761 ctatgaggcg aacggcggcc acggtctgcg gctccagccc ttccatcacg ccatggcgct
     5821 ctttcgcttc gccgtcatct tcgagggcat cgcggcccgg gcgcaggccg gcaatgcctc
     5881 ggccgacaac gccgccctgg tcggccgcct ctcggcccgc ttcgcccgcc gcgccgtcga
     5941 ggtgatcgag ggcatcgtgc acgtctgatc cggagggatg ccaatggaat tcgccttctc
     6001 cgccgaggtc gaggccctca gggaccagct ttcggccttc atggccgagc acgtcctgcc
     6061 ggccgatccg gcctttcgtg ccgccgcgtc gtcgggccgc cacgccgtcg agatcctcga
     6121 gccgctcaag gcccgggccc gggaagcggg cttgtggaac ctcttcctgc cggccttgcg
     6181 cgacgacgag cccggcacgc ggctgagcaa tctcgcctat gcgcccttgg ccgagatcat
     6241 gggccgggtg ccgtggtcgg ccgaggtctt caattgcagt gcccccgata ccggcaacat
     6301 ggagctcctg cacctctgcg ccacaccggc ccagcgcgaa cgctggctca tgccattgct
     6361 cgacggcacc atccgctcct gcgtcgccat caccgagccc ggcgtcgcct cgtccgatcc
     6421 gaccaacctg aaaacgacca tccgccgcga cggcgacacc ttcgtcgtca acggccgcaa
     6481 atggttcacc acgggcgccc tccacccggc ctgccgcttc gcgatcctca tgggcgtcac
     6541 cgacgagcgc cccgacgccg acccccatgc gcgccactcc atggtcatcg tgccgctcga
     6601 cacgcctggc gtggagatcg tccgcaacct gccgatcatg ggccatctcg cccccgaggg
     6661 tcattgcgag gtcgatttcg aggacgtccg catccccgcc gccaaccttc tgggcgaggc
     6721 gggcaggggc ttcgccctgg cccaggcgcg gctcggcccc ggccgggtgc atcactgcat
     6781 gcgctcgatc ggccagtgcg agctggcgct cgagctcatg gccgaccggg ccatggcacg
     6841 cgaggccttc ggccgcccgc tcgccgactt cgccaacatc caggactgga tcgccctctc
     6901 gcggatgcag atcgaccagg cccggctcta cgtgctccgc acggcctggc tcatggaccg
     6961 ggacggcaac aaggcggccc gggccgaggt ttccgccatc aagctcgtcg tctcgcgcct
     7021 gcagaccgag gtgctggacc gcgccatcca ggtcttcggt gccatgggcc tcaccgacga
     7081 cacgccgctc gcccatctct ggacctgggg cagagcactc cgcttcgtcg acggccccga
     7141 cgaggtgcat ctgcgcaccg tggcgagagc cgagctcagg cgggcgaggc accgggccac
     7201 gggtgccggc cccgggagct gagccgatgc gccgggatgc ccacctcgac cctcgccctg
     7261 ccaatttcgc cccgctcacg ccgctcgact tccttcgccg ctcgatcgag gtcttcccgg
     7321 gcaagaccgc cgtcgtcgcc ggcgcccgac ggctctccta cacggatctc ggcgagctgg
     7381 tcggccggct cgccgccggc cttcgccggt cggggatcgg ccggggcgat gtcgtctcgg
     7441 tgatcgccgg caacacgccg gagctgctcg ccctgcattt cgccgtgccg ctcacgggtg
     7501 cggtgctcaa cgccatcaac acccgcctcg atcccgccac cgtggcccac atcctggccc
     7561 acgccgaggc ccggctcgtc atggccgatg ccgagcgggc gccgctcacc cgcgccgctc
     7621 tcgcgcaagg tggcctcgag acgccgctcg tgaccctcga tgccctgtcc ctcgccgggt
     7681 tcacggaggg tctgccgccc ttgccggtca gcctcgacgc cgtcgccgac gaatggcagc
     7741 cgatcagcct caactacacc tccggcacca ccggcagccc caagggcgcc gtctaccacc
     7801 accgcggcgc ccatctgaac gcgctcggca atgtcatggc gctcggcttc gacgaacgga
     7861 gcgtctatct ctggacgctg cccatgttcc actgcaatgg ctggtgccat ccatgggccg
     7921 tgactgccgt cggcggcacg catgtctgcc tcgaccgacc cgatcccgcc gccatcgtgg
     7981 cggcgctccg ggaggaggcc gtcacccatt tcgcctgcgc gcccgtcgtc ctctacaacc
     8041 tcctgcatca gggtcgggag caaagcccaa gactgccgag gcgcgtgcgg gtcgccaccg
     8101 gtggcgcggc acccacgacg gcattgatcg agggcctcga cgccatgggc atcgagctga
     8161 tccatctcta tggcctgacc gagtccttgg gcccggtcac tgcttgcgcg gaacgcgccg
     8221 actggggtga gccggacgcc gcggagaagg cgcggctgct ggcgcgccag ggtgtgcgcc
     8281 acccgctcgc cgccgaggtc gccgtcgtcg atgcggcggg cgagccggtg ccgatggacg
     8341 gggccaccat gggcgagatc gtcatccgct cgaacacgct gatggcgggc tatctgaagg
     8401 acagggcggc caccgaggcc gctttccggg acggtcgtct caataccggc gacctggcgg
     8461 tgcgtcaccc cgacggcttc atccagatca gggaccgggc caaggacgtg atcatctcgg
     8521 gcggcgagaa catctcttcc ctggaggtcg agggtgtgct gcaccagcac ccggccgtgc
     8581 tgctcgccgc cgtcgttgcg gtcgcggacg agaaatgggg cgaggtgccg gtcgcctgcc
     8641 tcgagctcaa gcccggccag gcagcgccgc cagccgcgga gctcgaagcc ttctgtcgcg
     8701 accgtctggc ccacttcaag gtgccacgcc gtttcgtgct ccgcgagctg cccaggaccg
     8761 cgaccggcaa gatccagaaa ttccagctcc gcgccgagct ggcgttggat tgagcggcag
     8821 aaatcagccc aaggccggca cgagctcggc cgcggcggca tagcgctcgc caccctggcg
     8881 ccgggcttcg tccatcgccg cctcggcccg cgccaggagt gcccggaaat cgaggccggg
     8941 gccggatccg gtgaccacgc cgatgctcgg ccggacgcgc agcgtggcgt cgccgaccag
     9001 gatcggctcg cgcgcggcgg cgatgatctt gcgggccacg acataggcgt cgctgatgtg
     9061 cttcatgccg tcgatcaggg cgacgaaggt cgtgtcgtcg agcttggcga cggcgtcggt
     9121 gcgccgcatt tgccgcccga gccggtgcgc gagctcgacc agcgccgcct ctgcccgatc
     9181 gatgccgtgg gtctcgcgca ggctcgcgaa atcgtcgacg gcgatgtgca ggagggccag
     9241 atgggcgcgg ttgcgggcgg tgcgcgcaac cgcgcggccg aaccgttcct cgagctgcgg
     9301 cagccagggc aggccggtca gcgggtcacg caccgcaccc tggaccaggc ggtgctcgcg
     9361 cgccttgcgc tcgaccgcca gcttgagcgt gcggtagagc cggtcggcgt cgatccggcc
     9421 cttgcagaga aaatcctgcg ccccctgccg gatcgcctcg gtcgcgacca agaggtcgtc
     9481 ggcggcgaag ccggagagca ccaccacggc gagggccggg tgatgccgct gcagccattc
     9541 gaccccgttg agaccgcgcg cgtcgggcag ctccagatcg agcagcacga catcgggcgg
     9601 cgtggtggcg atcgcgtcgg ccgcatcgtg caggctggcg gccatgcgcg gcgccagacg
     9661 cgggcacgag gcgagctcgg cggcgaggag cgcccgctcg gcatcgctgt cctcgacaat
     9721 cagcaggttc aacgtgtcgg gcatggcccc ctggtcccgc ggcatctgac gcgatcctgg
     9781 acctcgggac ctaaaattcg gttaacgccc ggcgggcatc ggcgagccgt gctcagtcgc
     9841 gccagagata ggcgacctcg agcacccggg agccgccgat ctcgagcgtg ttctcggcga
     9901 cccgctcgcc gcccagatgc tcgtagaagc cgcgcgccgg gttgctcgcc agcacccaga
     9961 ccagggttcc ggcatagcgg cgccgcgcca gctcgaaatg acaggcatcg aagagcgcgc
    10021 ggccgatgcc ggcgccgtgc tggtcgggat gcacgtagag cacgtagatc tcgccgcccg
    10081 ccagcgcctg gcgccgcgac cgcccgccgc tggcgatgcc ga
//
LOCUS       DAOTFI010000184        10065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_7976_length_10065_cov_6.818182, whole genome shotgun sequence.
ACCESSION   DAOTFI010000184 DAOTFI010000000
VERSION     DAOTFI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7976_length_10065_cov_6.818182"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(547..1080)
                     /locus_tag="P5558_17100"
     CDS             complement(547..1080)
                     /locus_tag="P5558_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26089.1"
                     /translation="MAGPDLARRLCQAAIGDLIDWRTGGYDLARDATGAAFDRLFPCQ
                     RSKEQRLLDAALNGFDTHDLEDDTAAQLTAAASDLLLWHGLSSAQITQIAEADVGRWA
                     DRAMQTVCARGLRLNWHPAESAYCSALSILRMLDNNHPGEYHSLLANFVCGLDLTDGR
                     SAHHILRLSRHGAVRIS"
     gene            1204..1512
                     /locus_tag="P5558_17105"
     CDS             1204..1512
                     /locus_tag="P5558_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26090.1"
                     /translation="MCRAPFEALHRPAGAADVRHGTSTAGLTVFTRTTTVEYHLERSV
                     EQLQVGFGPIKGEASKGLLLGLLARQDRAVTLELMKEHRGLETDIMPDVLFTNAFVPQ
                     "
     gene            1551..2291
                     /locus_tag="P5558_17110"
     CDS             1551..2291
                     /locus_tag="P5558_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26091.1"
                     /translation="MAIVTGGAGGIGRAIAERLVAEGAAIALWDVDEGMAAATAAALR
                     LAGGEIEAVAVDVTAEASVEVATNATFERFGRVDLVVNNAGILGPVAPTWEHTPDAFR
                     RVLEVNLVGIFLVCRAVVPRLLSQDAPERCRIVNLASIQGKEGMPSAAAYVASKAGVI
                     GLTKTLGKELAKAGILVNCVTPSAVEGTGMATEITPERRAEIMGRIPMGRFVRLEEVA
                     AMVAWLGSLECTFSTGAVFDLSGGRATY"
     gene            2333..3490
                     /locus_tag="P5558_17115"
     CDS             2333..3490
                     /locus_tag="P5558_17115"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRY26092.1"
                     /translation="MSKHDFTAAEFAERRRRLRAALAKADLDWLVVVHPVAIRWLTGS
                     DAKSYQEFQCLFFSAEEAPLTVLTRAGEVAEFQDDALVDEVVGWGGGIAEDPIPHFTA
                     VAKRLGLMGARVGLDVPAFYLHPHHYLALREVLGTSLVAEPSTLVHDLTLVKSPAELA
                     FIRRAAALADDGMAVFARALRPGRSELALAGEVMANLMTAGSGIAASPVNLVSGERSG
                     FSHGAPTQRILRAGDCGSIEYGATWHRYTATIGRQFCLGPPTPRMLELHDIVRRAQDA
                     CIAAIRPGVSASVPHEAARAVIAEAGLEPYRVHTTGYGLAPGFPPSWGEPIHMLNDSP
                     YTLEAGMVLTVEPPVFIPAERLGARLIDNVLVTADGAERLTGASRELIVVE"
     gene            complement(3503..3910)
                     /locus_tag="P5558_17120"
     CDS             complement(3503..3910)
                     /locus_tag="P5558_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRY26093.1"
                     /translation="MAELTDAAIDAAIARGHDAASSEPRAKAAHYEPSGRRIVVELTN
                     GCTFAFPPDLAQGLEGASDMELEAVEILGAGYGLHWESLDVDLAVPDLMAGLFGTRAW
                     MARRAGRTTSPAKAEAARRNGAKGGRPRKPVVA"
     gene            complement(3951..4106)
                     /locus_tag="P5558_17125"
     CDS             complement(3951..4106)
                     /locus_tag="P5558_17125"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRY26094.1"
                     /translation="MIVANDHSPAHVHIFGDGHAKINLLGAAGAPELVWAEGMTRSVL
                     RRAMAVV"
     gene            complement(4232..4576)
                     /locus_tag="P5558_17130"
     CDS             complement(4232..4576)
                     /locus_tag="P5558_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY26095.1"
                     /translation="MIKRFKSGPRMSQAVVHGDTVYLAGQVADDFDSSLEQQTKEVLA
                     KIDGLLKEAGSSRDKILAVTVYLANIADFAAMNVVYDAWLDKANPPTRACLETRLADP
                     RLRVEIVAIAAA"
     gene            complement(4689..5042)
                     /locus_tag="P5558_17135"
     CDS             complement(4689..5042)
                     /locus_tag="P5558_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26096.1"
                     /translation="MAVVLVAALLVACTTSRPIDIGAGTDLGAKVKPEDYVEVTTIDG
                     QALAFEVLQVEPDALVGKGVRVERDQIGTLEVTEPSVGRTAAAVGGTGLAVFLIVGFV
                     VAAIAPALILSGAAP"
     gene            complement(5169..6110)
                     /locus_tag="P5558_17140"
     CDS             complement(5169..6110)
                     /locus_tag="P5558_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY26097.1"
                     /translation="MIGIDDIEKAQARIADRVRRTPVMALGQLKDPPPTRARVTLKLE
                     CLQVTGSFKARGAMNRLLGMAGGPPAAGIVTASGGNHGLAVARTAYAAGVPATIFVPS
                     GVSPEKVAKMHAWNARVEIVGDVWDRSNEAALAHAEESGAVYFHPFADPLVVAGQGTL
                     GLEILDQLPDIDTILIAIGGGGLIAGMSTAIRARRPNVRIIGIEPVGSPTLKASVDAG
                     EVVTLPSVTTRVPTMACARTDPRIYEIVRDKVDEFVLVDDEAMLDAARWLWFEQGIAA
                     DLSGAASLAALRQGIPSLAGAKHICALVCGAGPDGTG"
     gene            complement(6107..7138)
                     /locus_tag="P5558_17145"
     CDS             complement(6107..7138)
                     /locus_tag="P5558_17145"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRY26098.1"
                     /translation="MGHKATIRPSTAFLTVDPGKAGRQVGFLMIPHSPHDDAWGVTRV
                     PVAVIANGTGPTVILEGGNHGDEYEGPIAIGELIRELDPAEVQGRLILMPANNVHAVV
                     AGTRTSPVDGLNFNRTFPGDPRGTITQQITAYISDHLYPMADAFLDLHSGGSSLDILP
                     SAIIEPTEDAGLRRRNIAAVQAFDAPMTVVISNLGESRTATAAACQAGLITVGTEMGG
                     GGTVSLEALDVCRRGVRNVLVHLGVLDGKHRSPARGDGKILELPGSKAYVFATMDGIF
                     EPFHANGAAVSEGQPAGRIHCTWDATREPETVHYQTDGILYGRRQPGRVRPGNCCLVI
                     ATEYEGEVA"
     gene            complement(7212..7994)
                     /locus_tag="P5558_17150"
     CDS             complement(7212..7994)
                     /locus_tag="P5558_17150"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY26099.1"
                     /translation="MKTMLAAVTALAIGLVAAAPAKADKFEDILEAGVVRIGVPLDVP
                     PFGYQDADRNPVGFDVEMAQMIGEALGVEVELQQITGANRIPFLLTDKVDLVISVMGL
                     TPERAKQIMFSAPYANTFLAVYGPASASVSGPDEFGDLKVAAAKGTTQEMTLTAQNPD
                     ADIMRTEDDATAAAAYLSGQADLFATNSIVASALAEQNPNAEFDRKFVIRRSPAHIGV
                     AMGEFELLQWVNSFIFYNTMNGELERLHQTYLGIPMDPLPTL"
     gene            complement(8017..8748)
                     /locus_tag="P5558_17155"
     CDS             complement(8017..8748)
                     /locus_tag="P5558_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRY26100.1"
                     /translation="MALLEIEKITKRFGTNTVLDDVSLTVDAGEIIAVIGRSGSGKST
                     LLRCINGLEPIQGGRITVDGVRVDDKATDLRKLRQQVGIVFQSYNLFPHLSVSRNITL
                     APTLTKGITEAEAREIAREVLHRVGLEEKLDAYPEQLSGGQQQRVAIARSLAMRPKLM
                     LFDEITSALDPELTGEVLKVLEDMARSGMTMILVTHEMGFARRFGSRLVFMHEGRIHE
                     EGPAARTLAEPRTNELETFLGAVLH"
     gene            complement(8735..9385)
                     /locus_tag="P5558_17160"
     CDS             complement(8735..9385)
                     /locus_tag="P5558_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY26101.1"
                     /translation="MIREFGWIDVLYLVLAMRWTLALTVTAFVGGGLIGLVIAVLRVS
                     RVWPLQAIAVLYVQAIQGTPLLVLLFVFFFGLSIVGLPVLPWIAAALAFSIYAGAFLG
                     EIWRGALDAIPKTQWEAGAALGLGFGEQLRHIIVPQAIRMAIPPTVGFLVQLIKNTSL
                     AATIGFVELTREGQLTTASTFQPFAVYLTVAFLYFLTCFPLTQWSRHLERRSYGPA"
     gene            complement(9399..10061)
                     /locus_tag="P5558_17165"
     CDS             complement(9399..10061)
                     /locus_tag="P5558_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY26102.1"
                     /translation="MSYTFQFRDVFAAWEFILQGVAMTLYLSAVTMVLGLAIGVAGAA
                     GRLYGPKPLRLLVGAYVESIRNTPLLVQLFIVFFGLPSLGVRLDATTAAVVTLTVNLG
                     AYATEIVRAGLEAVPKAQVEAGHSLGLSGLQVFRHIVLFPALRMMYPALASQFILLML
                     ATSITSQIAVEDLFHVASIIQSRTFRDFEVYTVIGVLYLLLALVFRGGFALLYQLVFA
                     RR"
BASE COUNT         1634 a         3353 c         3479 g         1599 t
ORIGIN      
        1 ctcgccctgc tccgcggccg ctggcgcgaa cgcatcctcc taaaatcccc ccccgacccc
       61 gacctccccg acaagatcgc cgcctggctc gagccgctcc gcctgccggg tacgctacgg
      121 ctgcaggtgg acatcgatcc gcagagcttt ttttgacgtc cctgccgata agggccgttt
      181 ggcacctgac cggcgcgcgt tcgtcgctcg aatgggtctc ggatgtcaga acgctataac
      241 gaatcggtag atggaactcg gttcgagaat cttgagaatc aaagcgaagc tcgatgagat
      301 tttatcctcg ccagctctct ttcgatcgga tccaaaagtt gggcatctgg tcttcgccct
      361 ctggtttcac acaagcgtcg gtacatttta atcataggct cagtgatttg ttcgtatctt
      421 ttgggattcg aaagaaacag cggtccctgc cattcgatgg ccattttcac gctcttcagt
      481 gcagtcatat taaatccgac cttctccaag aattccgctc gaattgccaa ggttagtgct
      541 agacactcac gatattcgta ccgccccgtg tctggaaagt cggagaatat ggtgagcgct
      601 tcgtccatca gtcaaatcaa gaccacaaac gaaattggcg agcaatgaat gatattcacc
      661 gggatgatta ttatcaagca tccgaagtat agagagtgcc gaacaatagg ctgattctgc
      721 tggatgccaa tttaaccgca acccccgcgc gcagaccgtc tgcatcgccc gatcggccca
      781 gcgccccaca tcggcttcgg cgatttgcgt gatctgcgcc gacgagagcc catgccagag
      841 caggaggtcg cttgcggcgg cggtcagttg cgccgctgta tcgtcctcga ggtcgtgcgt
      901 gtcgaaaccg ttcaacgccg catcgagcag gcgctgttcc ttgctccgtt gacagggaaa
      961 aaggcggtcg aaagccgcgc cagttgcgtc gcgcgcaagg tcgtagccgc ccgtgcgcca
     1021 gtcgatcagg tcgccgatcg ctgcctgaca gagccgtctg gctaaatccg gcccggccaa
     1081 accacaacct ccggctcggc tccggcgtca gttagatcgc agatcgcgcg cctttggaaa
     1141 gcctcgcccg tcagtctctc caaccagatt ggcgcggcgt cgcgcgagtt catcgcaacc
     1201 gagatgtgcc gggcgccctt tgaagccctg catcgtcctg ccggcgcggc cgacgtcagg
     1261 cacggcacca gcacggcggg cctgaccgtc ttcacccgaa ccaccacggt cgagtaccat
     1321 cttgagcgct cggtggagca gcttcaggtc ggcttcgggc cgatcaaggg cgaggcgtcg
     1381 aaaggcttgc ttttgggcct gctcgcgcgt caggatcggg ccgtcacgct ggagctcatg
     1441 aaggagcaca gggggctgga gaccgacatc atgccggacg tgctcttcac caacgcgttc
     1501 gtgccgcagt gacggcgggg ccagcatcac ccgcccgcca tgccgggcgg gtggccatcg
     1561 tcaccggcgg cgccggcggc atcggccggg cgatcgccga acggctcgtc gccgaggggg
     1621 ccgcgatcgc gctctgggac gtcgacgagg gcatggcggc ggctacggcg gcggcgctgc
     1681 gccttgccgg cggcgagatc gaggccgtcg ccgtcgacgt gaccgccgag gcttccgtcg
     1741 aggtcgccac caacgccacc ttcgagcgct tcggccgcgt cgacctcgtg gtgaacaatg
     1801 ccggcatcct cggccccgtg gcgcccacct gggagcacac gccggacgcg ttccgccggg
     1861 tgctcgaggt caatctcgtc ggcatcttcc tcgtctgccg ggccgtcgtg ccgcggcttc
     1921 tgtcacagga cgcgccggag cgctgccgca tcgtcaacct cgcctccatc cagggcaagg
     1981 agggcatgcc ctcggccgcc gcctacgtcg cctccaaggc cggcgtgatc gggctcacca
     2041 agacgctcgg caaggagctg gccaaggccg gcatcctcgt caactgcgtc accccctcgg
     2101 ccgtcgaggg caccggcatg gcaaccgaga tcacgcccga gcgccgcgcc gagatcatgg
     2161 gccgcatccc gatgggccgc ttcgtccgcc tggaggaagt ggccgccatg gtcgcctggc
     2221 tcggcagcct ggaatgcacc ttctcgaccg gcgccgtttt cgacctctcg ggcggccggg
     2281 cgacctattg aactctcccg tggcgagcgg gcgatcaacg gacaggcgcc ggatgtccaa
     2341 gcacgatttc accgccgccg agttcgccga aaggcgaagg cggcttcgcg cagcactggc
     2401 gaaggcggac ctcgactggc tcgtcgtcgt ccacccggtg gcgatccgct ggctgaccgg
     2461 ctcggacgcc aagagctacc aggagttcca gtgcctcttc ttcagcgccg aggaggcgcc
     2521 gctcaccgtc ctgacccggg ccggcgaggt ggcggaattc caggacgacg cgctggtcga
     2581 cgaggtggtg ggctggggtg gcggcatcgc cgaggacccc atcccgcatt tcacggcggt
     2641 ggcgaagcgg ctcgggctca tgggcgcccg ggtcggcctc gacgtgccgg ccttttacct
     2701 ccatccgcac cactatctgg cgctgcgcga ggtgctgggc accagcctcg tggcggagcc
     2761 ctccaccctg gtccacgacc tgacgctggt gaaatcgccg gccgagctcg ccttcatccg
     2821 ccgggcggcc gccctcgccg acgacggcat ggcggtcttc gcccgggcgc tccggccggg
     2881 ccgcagcgag ctcgcgctgg ccggcgaggt gatggcgaac ctcatgaccg ccggcagcgg
     2941 catcgccgcc agcccggtca acctcgtctc cggcgagcgg tccggcttca gccacggcgc
     3001 ccccacccag cgaatcctgc gggccggcga ctgcggcagc atcgagtacg gcgccacctg
     3061 gcaccgctac accgccacca tcggccgcca gttctgcctc ggcccgccga caccacgcat
     3121 gctggagctc cacgatatcg tccgccgggc gcaggatgcc tgcatcgccg ccatccggcc
     3181 gggcgtgagc gccagcgtcc cgcacgaggc cgcccgtgcg gtgatcgccg aagccgggct
     3241 cgagccctac cgcgtccata ccaccggcta cggcctggca cccggcttcc cgccgagctg
     3301 gggcgagccc atccacatgc tcaacgacag cccctacacg ctcgaagccg gcatggtcct
     3361 cacggtcgag cccccggtct tcatccccgc ggaacggctg ggtgcgcggc tgatcgacaa
     3421 cgtgctggtc acggcggacg gggcggagcg tctgacgggg gcgagtcggg agctgatcgt
     3481 ggtagagtga agggcggcgc gttcaggcta ccacgggctt cctcggccga ccgcccttcg
     3541 ctccgttgcg gcgggcggcc tcggccttgg cgggtgaggt ggtgcggccg gcccggcggg
     3601 ccatccaggc tcgggtgccg aagaggccgg ccatgaggtc gggaacggca aggtcgacgt
     3661 ccaggctttc ccagtgcagg ccatagccgg cgccgagaat ctcgacagct tcgagctcca
     3721 tatcgctcgc cccctcgagt ccctgggcga ggtccggcgg aaaggcgaag gtgcagccat
     3781 tggtgagctc gacgacgatc cggcgaccgc tgggttcgta gtgtgccgct ttggcgcgcg
     3841 gctcgctcga agcggcatca tgcccacggg cgatggccgc atcgatggcg gcatcggtca
     3901 actcagccat gcagctccct ccagcgcacc agcagatcct gcaacgcgtc ctagacgacc
     3961 gccattgccc ggcgcagcac gctccgcgtc atgccctcgg cccagaccag ctcgggtgcg
     4021 ccggcggcgc cgagcagatt gatcttcgcg tgaccatctc cgaagatatg gacatgcgcc
     4081 ggtgaatggt cgttggcgac gatcactatc cgcagaccgc gtgcgcgacg gaccgtgacc
     4141 aaaccaataa cccatccgga taggttattc aagcctgacg gtacggcagt gaaaggcggc
     4201 gagagaagac ggctcccgcc gccttcacag ctcaggccgc tgcgatcgcg acgatctcga
     4261 cgcggagccg cgggtcggcg aggcgggtct cgaggcaggc gcgggtcggc gggttcgcct
     4321 tgtcgagcca ggcgtcgtag accacgttca tggcggcgaa atcggcgatg ttcgcgagat
     4381 agaccgtgac tgccaggatc ttgtcgcggc tgctgccggc ctccttgagc aggccgtcga
     4441 tcttggccag gacctccttc gtctgctgct cgaggctgga gtcgaagtcg tcggccacct
     4501 ggccggcgag gtagacggtg tcgccgtgga cgacggcctg gctcatgcgg gggccggact
     4561 tgaagcgctt gatcatcggt tttcctgcag gttgtcgaaa gctgcgcgcc ggcttcgccg
     4621 gagcggccgc accttgccgc aagcgagcct ttctgtcgcc ctcccgattc cgccgagctc
     4681 gggccgttct agggcgcggc accggagagg atcagggccg gtgctatggc ggcgaccacg
     4741 aagccgacga tcaggaagac cgcgaggccc gtgccgccta cggccgcggc cgtgcgcccc
     4801 acgctgggct cggtcacctc gagcgtcccg atctggtcgc gctccacccg cacgcccttg
     4861 ccaacgaggg cgtccggctc gacctgcagg acctcgaagg cgagcgcctg cccgtcgatc
     4921 gtggtgacct cgacgtagtc ctcgggcttc accttggcgc cgaggtcggt cccggcaccg
     4981 atgtcgatcg gccgggacgt cgtgcaggcc acgagcaggg cggcgaccag gacgacagcc
     5041 atcgagcgcc tcgaccgttg ccgaacgcac gctcgctcgc ccatggcgcg acccttaatc
     5101 tatatcaact tcagaatatt gaacaataac atgggatcgg ggatgggcac aagcgtggtg
     5161 ctgtggtctc acccagtgcc gtcggggccg gcgccgcaga cgagggcgca gatgtgcttc
     5221 gcaccggcga gggaggggat accctggcgc agtgcggcga gcgaggccgc accggagaga
     5281 tcggcggcga tgccctgctc gaaccacagc cagcgggccg cgtccagcat cgcctcgtcg
     5341 tcgaccagga cgaattcgtc gaccttgtcc cgaacgatct cgtagatgcg gggatcggtg
     5401 cgcgcgcagg ccatggtcgg cacgcgcgtg gtgacagaag gcagggtcac gacctcgccc
     5461 gcatcgaccg aggccttgag cgtcggcgag cccacgggct cgatgccgat gatcctgacg
     5521 ttcggccggc gagcgcggat ggcggtcgac atgccggcga tcagcccgcc gccgccgatg
     5581 gcgatcagga tggtgtcgat atcgggaagc tggtcgagga tctcgagccc gagcgtgcct
     5641 tgccccgcca ccaccagcgg gtcggcgaaa gggtggaaat agaccgcacc gctttcctcg
     5701 gcatgcgcca gcgccgcctc gttggagcgg tcccagacat cgcccacgat ctcgaccctg
     5761 gcgttccagg cgtgcatctt cgccaccttc tcgggcgaca cgccggaggg cacgaagatg
     5821 gtggccggca cccccgccgc ataggccgtg cgcgccacgg cgagcccatg attgccgccc
     5881 gaggccgtga cgatgccggc cgccggcggc ccgcccgcca tgccgagcag gcggttcatg
     5941 gcccccctcg ccttgaacga gccggtcacc tgcaggcatt cgagcttcag cgtcacccgc
     6001 gctctcgtcg gcggcgggtc cttgagctgg cccagcgcca tgaccggtgt gcggcgcacc
     6061 cggtcggcga tgcgcgcttg cgccttctcg atgtcgtcga tgccgatcat gcgacctcgc
     6121 cttcgtattc cgtggcgatc acgaggcagc aattgcccgg ccggacgcgg cccggctggc
     6181 ggcggccata gaggatgccg tcggtctggt agtggaccgt ctcgggctcg cgggtggcat
     6241 cccaggtgca gtggatgcga ccggccggct ggccctcgct gaccgccgca ccgttcgcgt
     6301 ggaagggctc gaagatgccg tccatggtgg cgaagacata ggccttcgag cccggcagct
     6361 cgagaatctt gccgtcaccc cgggcggggc tcctgtgctt gccgtcgagc acgccgaggt
     6421 gcacgagcac gttcctgaca ccgcgccggc agacgtcgag cgcctccagc gacacggttc
     6481 caccgccgcc catctcggtg ccgacggtga tcagccccgc ctggcaggcg gcggcggtgg
     6541 cggtgcgcga ttcgccgaga ttgctgatca ccaccgtcat cggcgcgtcg aaggcctgga
     6601 cggcggcgat gttgcgccgg cgcagcccgg cgtcctcggt cggctcgatg atggccgacg
     6661 gcaggatgtc gagcgacgag ccgccggaat ggaggtcgag gaaggcgtcg gccatcggat
     6721 agaggtggtc gctgatatag gccgtgatct gctgggtgat ggtgccccgg gggtcgcccg
     6781 ggaaggtgcg gttgaagttc agcccgtcga ccggcgaggt gcgggtgccg gccacgaccg
     6841 catgcacgtt gttggcgggc atcaggatca gccgcccctg gacctcggcc gggtccagct
     6901 cgcggatcag ctcgccgatg gcgatcggcc cctcgtactc gtcgccgtga ttgccgccct
     6961 cgaggatcac cgtcgggcct gtcccgttgg cgatgaccgc gaccggcacg cgggtgacac
     7021 cccaggcgtc gtcgtgcggc gaatgcggga tcatgaggaa gcccacctgc cggccggcct
     7081 tgccgggatc gaccgtgagg aaggcggtgg agggacggat ggtggccttg tggcccatgg
     7141 tatcggtctc cgcgcgcgcc gggcggcggt tttagccgcc gcccggggag cattcgtcgt
     7201 cgaaaggtgg gtcagagggt cggcagcgga tccatcggga tgcccagata ggtctggtgc
     7261 agccgctcga gctcgccgtt catggtgttg tagaagatga acgagttcac ccactgcagg
     7321 agctcgaact cgcccatggc gacgccgata tgcgccgggc tccggcggat gacgaacttg
     7381 cggtcgaact cggcgttcgg gttctgctcg gccaaggcac tggcgacgat gctgttggtg
     7441 gcgaagagat cggcctggcc cgagagatag gccgccgccg ccgtcgcgtc gtcctcggtg
     7501 cgcatgatgt cggcatcggg gttctgcgcg gtcagcgtca tctcctgcgt ggtgcccttg
     7561 gcggccgcca ccttgagatc gccgaactcg tccgggcccg agacgctggc cgaggccggg
     7621 ccgtagaccg cgaggaaggt gttggcgtag ggggccgaga acatgatctg cttggcgcgc
     7681 tcgggtgtga gccccatcac cgagatgacc agatccacct tgtcggtgag caggaagggg
     7741 atgcggttcg ccccggtgat ctgctgcagc tcgacctcga cgccgagcgc ctcgccgatc
     7801 atctgcgcca tctcgacatc gaagcccacg ggattgcggt cggcgtcctg atagccgaag
     7861 ggcggcacgt cgagcggcac gccgatgcgg acgaccccgg cctcgaggat gtcctcgaac
     7921 ttgtcggcct tggccggcgc cgcggcgacg aggccgatgg ccagtgccgt gacggcggcg
     7981 agcatggtct tcatgacagt ctcctctccc gttggctcag tgcaggaccg caccgaggaa
     8041 ggtttcgagc tcgttggtcc tgggctccgc cagggtccgg gcggcgggcc cctcctcgtg
     8101 gattcgcccc tcgtgcatga agacgaggcg cgagccgaag cggcgggcaa agcccatctc
     8161 gtgggtgacg aggatcatcg tcatgccgct tctcgccatg tcctcgagca ccttcaggac
     8221 ctcgccggtc agctccgggt cgagggccga ggtgatctcg tcgaacagca tgagcttggg
     8281 ccgcatggcg agcgagcggg cgatggcgac gcgctgctgc tggccgcccg agagctgctc
     8341 cggataggcg tcgagcttct cctcgaggcc gacccgatgc agcacctcgc gcgcgatctc
     8401 gcgcgcctcg gcctcggtga tgcccttggt gagcgtgggg gcgagggtga tgttccgcga
     8461 caccgagaga tgcggaaaga gattgtagct ctggaagacg atgccgacct gctgccgcag
     8521 cttccggagg tccgtcgcct tgtcgtcgac ccgcacgccg tcgacggtga tgcggccccc
     8581 ctggatcggc tcgagcccgt tgatgcagcg gagcagcgtg ctcttgccgg agcccgagcg
     8641 gccgatcacg gcgatgatct cgccggcatc gaccgtgagc gacacgtcgt cgagcaccgt
     8701 gttcgtgccg aagcgcttgg tgatcttctc gatctcaagc agggccatag gatctccgct
     8761 cgaggtgccg gctccactgg gtcaggggga agcaggtgag gaagtagagg aaggccacgg
     8821 tcaggtacac ggcgaagggc tggaaggtcg aggccgtggt gagctggccc tcgcgggtca
     8881 gctcgacgaa gccgatcgtg gcggcgagcg aggtgttctt gatgagctgg acgaggaagc
     8941 ccacggtcgg cgggatggcc atgcggatgg cctggggcac gatgatgtgg cgaagctgct
     9001 cgccaaagcc gagcccgagc gcggcgccgg cctcccactg ggtcttgggg atggcgtcga
     9061 gcgccccccg ccagatctcg ccgaggaagg cgccggcata gatcgagaac gcgagggcgg
     9121 cggcgatcca gggcagcacc ggcaggccca cgatcgagag cccgaagaag aagacgaaga
     9181 gcagcacgag caagggcgtg ccctggatcg cctggacgta gaggaccgcg atcgcctgga
     9241 gcggccagac gcgcgagacc ctgagcacgg cgatgaccag cccgatcagc ccgccgccga
     9301 cgaaggcggt gacggtgagc gccagcgtcc agcgcatcgc gaggacgaga tagagcacgt
     9361 cgatccagcc gaactcccgg atcatggtgc catgcccgtc atcgccgcgc gaacacgagc
     9421 tggtagagaa gcgcgaaccc gcccctgaag acgagggcga gcagcaagta gagcacgccg
     9481 atgaccgtgt agacctcgaa gtcgcggaac gtgcgcgact ggatgatcga ggccacgtgg
     9541 aagaggtcct cgacggcgat ctgcgaggtg atgctggtgg ccagcatcag aaggatgaac
     9601 tggctggcga gcgcggggta catcatccgc agcgccggga agaggacgat gtggcgaaag
     9661 acctgcaggc ccgagaggcc gagcgaatgg cccgcctcga cctgcgcctt gggcaccgcc
     9721 tccagcccgg cccggacgat ctcggtggcg taggcgccga gattgacggt cagcgtgaca
     9781 acggcggccg tggtcgcgtc caagcgcacg ccgaggctcg gcaggccgaa gaagacgatg
     9841 aagagctgca ccagcagcgg cgtgttcctg atcgactcga cgtaggcgcc gaccaggagg
     9901 cggagcggct tcggcccgta gagccggccg gccgcgccgg cgacgccgat ggcgaggccg
     9961 agaaccatgg tgacggccga gaggtagagc gtcatcgcca cgccctggag gatgaactcc
    10021 caggcggcga acacgtcgcg gaactggaac gtgtagctca agcct
//
LOCUS       DAOTFI010000185         9997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8037_length_9997_cov_6.370952, whole genome shotgun sequence.
ACCESSION   DAOTFI010000185 DAOTFI010000000
VERSION     DAOTFI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8037_length_9997_cov_6.370952"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..86)
                     /locus_tag="P5558_17170"
     CDS             complement(<1..86)
                     /locus_tag="P5558_17170"
                     /inference="COORDINATES: protein motif:HMM:NF021580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HRY26103.1"
                     /translation="MTDVGGVTAERLRSFIERVERLEEEKSEV"
     gene            complement(379..2049)
                     /locus_tag="P5558_17175"
     CDS             complement(379..2049)
                     /locus_tag="P5558_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY26104.1"
                     /translation="MALSERHAAPVAREPESVTVEALVAADALLLAAREAVAARVAPG
                     GKVDPALLDAGQFAAHGLAWLEVYRQALHAMHAWGASLAAAGGLGELERLMLEAAFGE
                     YLARMAGGLPMSQVEVVRPADLHLDDRALQAFTGNPAVRTLIAKGNTAQRRQRLAELI
                     EDGDFGGWALGDDTLDMTREQFRRFTDEQVVPQAQAWHQRDELIPLELIGELAALGVF
                     GLTLPEAHGGLGMGKVAMCVVTEELSRGYIGVGSLPTRSEIAGELIRAGGTPEQQARF
                     LPRIASGEIIPTAVFTEPDTGSDLASLKTRAVRDGEGWSITGAKTWITHAARADLMTV
                     LCRTRPDAAGYRGLSMLLAEKPRGSDADPFPVAGLSGSEIPVLGYRGMKEYTLAFDGF
                     RVAGDNLLGGVEGQGFKQLMATFEGARIQTAARAVGVARSALELGLAYARERRQFGQP
                     LIAFPRVSGKLVWMAVEIQIARQLTFSAAREKDAGRRCDLEAGLAKLLAAQVAWAAAD
                     NALQIHGGNGYAVEYPISRVLCDARILSIFEGAAEIQAQVIARRLLED"
     gene            complement(2059..3270)
                     /gene="ccrA"
                     /locus_tag="P5558_17180"
     CDS             complement(2059..3270)
                     /gene="ccrA"
                     /locus_tag="P5558_17180"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HRY26105.1"
                     /translation="MPPLGHVPANMYAWAIRKERHGEPLTAMQQEVVPTPVVGDDEVL
                     VMVMAAGVNYNGVWAALGVPLSPLDLHKQPYHIAGSDAAGVVWAVGAKVRRWQVGDEV
                     VIHCNQDDGDDEECNGGDPMNSASQRIWGYETTDGSFAQFCKVQSRQLMPRPRHLTWE
                     EAGCYTLVLATAYRMLFGHQPHTLKPGDNVLVWGGAGGLGSMAIQLIAASGANAIAVI
                     SDEDKRDFVLSLGARGAINRKDFSCWGEMPDPMDAEAYGKWVKEARRFGKAIWEITGK
                     GNDVDMVFEHPGASTFPVSALVCKRGGMVVICAGTTGYKLTVDARYLWMRQKRLQGSH
                     FANLKQASAANRFVLARQVDPCMSEVFAWVDIPRAHTKMLKNQHKPGNMAVLVNARRT
                     GMRTLEEAMEG"
     gene            3878..>4587
                     /locus_tag="P5558_17185"
     CDS             3878..>4587
                     /locus_tag="P5558_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRY26106.1"
                     /translation="MGETSGINTFRDEPWLFRTYAGHSSAEASNALFRTNLARGQTGL
                     SVAFDLPTQTGYDSDDPLAQGEVGKVGVPITSLDDMTTLFDGIPLDRMNTSMTINATA
                     PWLLALYIAAAERQGVDRAVLQGTVQNDIIKEYLSRGTYIFPPAPSLRLIGDVVAFTC
                     RDVPRWNPTNVCSYHLQEAGATPVQELAFALATAVAVLDAIRAGGQVPEAEFPKVVGR
                     ISFFVNAGLHFVTELAKMR"
     assembly_gap    4588..4687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4688..5755
                     /locus_tag="P5558_17190"
     CDS             <4688..5755
                     /locus_tag="P5558_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRY26107.1"
                     /translation="GLPRPWDQQWSLRLQQILALETDLLDHPDIFDGSIVIEAKVQEL
                     MAEAKAELARIEDMGGALAAVDSGYMKSRLVESSAARVQAIAAGTRQVVGVNCYTETA
                     PSPLTTGEDGGFLTVDPATEAAQIQRLRAHRARRDAGTVEVALAELDRAAREGQNVMP
                     ASIACAHAGVTTGEWATCLRRIFGEYRAPTGIAGAKTDGLDDALAPVRARVEALARRT
                     GRRLKILVGKPGLDGHSNGAEQIAVAARDSGMDVVFEGIRLSPARIVGTALEEAVHVV
                     GLSILSGSHLQLVEEVMTRMRKAGIGDIPVVVGGIIPPADAKALLACGVARVYTPKDF
                     SIPRIMADIVDIAVSAESQAA"
     gene            complement(5864..6709)
                     /gene="radC"
                     /locus_tag="P5558_17195"
     CDS             complement(5864..6709)
                     /gene="radC"
                     /locus_tag="P5558_17195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRY26108.1"
                     /translation="MNVDLAICDGRYALADEANGGPVFYPTHRARHAELAEAPVTATF
                     DKLAALGREKAQDWEHWRKLRRRFLEGGLEVLSDSELLEIFLAVSSPRPKRQSQLAAA
                     LISRFGNLTGVVHANPRHLMEFPTENRRATYMAIALLKCVHAAAVRLLKVDVVDRPII
                     SSSEAVLRYCMARFSHLGHEEFHVLFLNRKNMLILDEVQQRGTIDHVPLYPREVAKRC
                     LEVGASAIVMVHNHPSGDPTPSGPDIELTRKVVAALEIFDVQVHDHFIIGLDGQASLR
                     GLGLM"
     gene            complement(6847..7611)
                     /locus_tag="P5558_17200"
     CDS             complement(6847..7611)
                     /locus_tag="P5558_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY26109.1"
                     /translation="MMDRAAAGQSWSATGYRHHAGFVATLGRPVLELLAPVAGERILD
                     LGCGDGALTAELVATGAEVVGCDASPELLDAARARGLDVVLADGHALPFEAEFDAVFS
                     NAALHWMTRPDDVIAGVARALRPGGRFVAEFGGMGNVAAITTALRAVLGEAGIDAFAM
                     RPWYFPSPDEHAARLERQGFSVSSIELIPRPTPLPTGMAGWLETFAQPFLRHAPAVER
                     AGLLARAVRLLEPALCDSAGNWTADYVRLRFRAARG"
     gene            complement(7801..9000)
                     /locus_tag="P5558_17205"
     CDS             complement(7801..9000)
                     /locus_tag="P5558_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26110.1"
                     /translation="MSAVFLEADGLAGNRLKRAPLGNGHDERWLQDLLFRHPHLIPLE
                     RIEPGAGEVIPICRELSLPKPGGRSVFLDILGVTPHGRPVLVECKLWRNPQARREVVA
                     QLLEYAALLRRRSFADLTVQLRAVLGTKDDNPLFTHVRHGLSEPADEVRFVDAVARNL
                     RAGDFHLIIAGEGIREDLTVISEHLGAQGARLALIELQLWCDDGGRTLVVPQVAMRTE
                     VIRQRLILDDAGIPVQLVDLAGSDDRTEIEEVVDPEGARIRRESNREFWQRFIETACF
                     EHPDQPTPRHGTNNSVRIPLPPPARWLTAYRSEGEGEAGLFLRCEQGNMLCAELAPEA
                     EALRAETGLRDLYFKVGQPGQRDIIGIARPLTDFGGTDEQLAWLECTANKLVNALRPR
                     LSVPQDA"
     gene            complement(9140..>9997)
                     /locus_tag="P5558_17210"
     CDS             complement(9140..>9997)
                     /locus_tag="P5558_17210"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRY26111.1"
                     /translation="HGATGKGNDQVRFELGCYALDPHIKVIAPWRLWDLNSRDKLIQY
                     AESRQIPIPRGKRGTPPYSTDANLLHISYEGLALEDPWIEADEEMFTRSVSPELAPER
                     PTYVEIGFERGDAVSVDGEPLSPANLLRRLNDLAGANGIGRLDLVENRYVGMKSRGVY
                     ETPGGTILLAAHRGIESITLDRGAAHLKDELMPRYAELIYNGYWFSPEREMLQALIDQ
                     SQGPVTGTVRLKLYKGSAAVVGRKAPQSLYSEDYATFEADTVYDQKDAEGFIKLNALR
                     LRIAALARR"
BASE COUNT         1603 a         3345 c         3297 g         1652 t
ORIGIN      
        1 acctcgctct tctcctcctc gagacgctcg acccgctcga tgaagctgcg caggcgctcc
       61 gccgtcacgc cgccgacatc cgtcatccgc tctgcaactc cgctggcttc gccgccacgg
      121 tcgcgatcgc ggcaggctga acacgaaaca gaggggagag agccggagcg tccccggctc
      181 tctccccttg gcctcaatgg cattgaccgg caacggcagg accgtcgcgc ggttgcctcc
      241 cttttcctac ccggccggga ccgtcgccag ggcgaccggc tcgccgctcg cgccggacag
      301 gaaccggcgc gacagaaggc cgcgtagcac gcggccacgc agttctccag tgcttcgcgc
      361 gacaagaggc attctgcctc agtcctcgag cagccgccgc gcgatcacct gcgcctggat
      421 ttcggcagca ccctcgaaga tgctgaggat gcgggcatcg cagaggacgc ggctgatcgg
      481 gtactcgacg gcgtagccgt tgccgccgtg gatctgcagc gcgttgtcgg ccgctgccca
      541 ggcgacctgg gcggcgagca gcttggcgag cccggcctcg aggtcgcagc gtcgccccgc
      601 gtccttctcg cgcgccgcgg agaaggtgag ctggcgggcg atctggatct cgacggccat
      661 ccagacgagc ttgccggaga cccgtggaaa cgcgatcagc ggctgtccga actggcggcg
      721 ctccctggca taggcgaggc ctagctcgag cgcagagcgg gcgaccccga cggccctggc
      781 cgcggtctgg atccgagccc cctcgaaggt cgccatgagc tgcttgaagc cctgcccctc
      841 gacgccaccc agcaggttgt cgccggcgac gcgaaagccg tcgaaggcga gcgtgtactc
      901 cttcatgccg cgatagccga ggaccgggat ctcggagccg ctaaggccgg caaccgggaa
      961 tgggtcggca tcgctgccac gcggtttttc ggcgagcagc atcgaaagcc cgcgatagcc
     1021 cgccgcgtca gggcgagtgc gacagagcac ggtcatgaga tcggcgcgcg ccgcgtgggt
     1081 gatccaggtc ttggccccgg tgatcgacca gccctcgccg tcgcgcacgg cgcgggtctt
     1141 gagcgaggcc aggtcggagc ccgtgtcggg ctcggtgaag accgcggtcg ggatgatctc
     1201 gccggaggcg atccgcggca ggaagcgggc ctgctgctcg ggcgtgccgc cggcgcggat
     1261 cagctcgccc gcgatctccg agcgggtcgg cagtgagccc acgccgatat agccgcggct
     1321 cagctcctcg gtgacgacgc acatcgccac cttgcccatg ccgagcccgc catgcgcctc
     1381 cggcagcgtc aggccgaaga cgccgagggc ggccagctct ccgatgagct cgagcgggat
     1441 cagctcgtcg cgctggtgcc atgcctgcgc ctgcggcacg acctgctcgt cggtgaagcg
     1501 ccggaactgc tcgcgggtca tgtcgagcgt gtcgtcaccg agcgcccagc cgccgaaatc
     1561 gccgtcctcg atcagctcgg cgaggcgctg gcggcgttgt gccgtgttcc ccttagcgat
     1621 cagggtccgg accgccgggt tgccggtgaa tgcctggagc gccctgtcgt ccagatgcag
     1681 gtcggcgggg cgcacgacct cgacctggct catcggcagg ccgcccgcca tgcgcgccag
     1741 atactcgccg aaggccgcct ccagcatcag tcgttcgagc tcgccgagcc cgccggcggc
     1801 cgccagactt gccccccagg cgtgcatggc gtgcaacgcc tggcggtaga cctcgagcca
     1861 ggcgagccca tgcgcggcaa actggcctgc gtcgagcaag gccgggtcga ccttgccgcc
     1921 cggtgccacc cgtgcagcga cggcttcccg tgcggcgagg agcagcgcat cggcggcgac
     1981 gagcgcttcg acagtgacgg attcgggctc cctggccacc ggcgccgcgt gccgctcgga
     2041 gagtgccatc gccaaggatc agccctccat cgcttcctcg agggtgcgca tgcccgtccg
     2101 ccgggcattg accaggaccg ccatgttgcc cggcttgtgc tggttcttga gcatcttcgt
     2161 gtgcgccctc gggatgtcta cccatgcgaa cacctcgctc atgcagggat cgacctgccg
     2221 ggcgagcacg aaccggttgg cggccgaggc ctgcttgagg ttggcgaagt ggctgccctg
     2281 caggcgcttc tgccgcatcc agaggtagcg ggcatcgacg gtcagcttgt agccggtggt
     2341 gccggcgcag atgaccacca tgccgccccg cttgcagacc agggccgaga ccgggaaggt
     2401 cgaggcgccg gggtgctcga agaccatgtc gacatcattg cccttgccgg tgatctccca
     2461 gatcgccttg ccgaagcggc gcgcctcctt gacccacttg ccgtaggcct cggcgtccat
     2521 ggggtccggc atctcccccc agcacgagaa gtccttgcgg ttgatcgccc ccctggcgcc
     2581 gagcgagagg acgaagtcgc gcttgtcctc gtccgagatg acggcgatgg cattggcacc
     2641 ggaggcggcg atgagctgga tcgccatgga gccgaggccc cccgccccgc cccagaccag
     2701 cacgttgtcg ccgggcttga gggtgtgcgg ctggtggccg aagagcatgc ggtaggcggt
     2761 ggccaggacc agggtgtagc agccggcttc ttcccaggtc aggtgccggg ggcgcggcat
     2821 gagctggcgc gactggacct tgcagaactg ggcgaaggag ccgtccgtgg tctcgtagcc
     2881 ccagatgcgc tgcgaggcgg agttcatggg gtcgccgcca ttgcactcct cgtcgtcccc
     2941 gtcgtcctgg ttgcagtgga tgacgacctc gtcgccgacc tgccagcgcc tgaccttggc
     3001 gccgaccgcc cagaccaccc cggcggcatc ggagccggcg atgtggtaag gctgcttgtg
     3061 cagatcgagg ggcgagagcg gcacgccgag cgcggcccag acgccgttgt agttgacgcc
     3121 ggcggccatc accatgacca gcacctcgtc gtcgccgacc acgggtgtgg gcaccacctc
     3181 ctgctgcatg gcggtcaacg gctcgccgtg ccgctccttg cggatcgccc aggcgtacat
     3241 gttggccggc acatgcccca gaggcggaat ctcgccgacc tcgtagagat ccttgccggc
     3301 agccggcgcg gcgggcgccg cgggcgcggc cgccggcggc ggggcctccg gcttcaaccg
     3361 gatcacctgg gccgatgcgg gtgcggccgg ggcgctgccg ccctggcggg ccatcggcag
     3421 cggccgtccg tgcgtgccgg tcgcctcggc cgcgaagaac tcgccgggcg acagggcgat
     3481 gccctcgcgg cgggcgatct cgagcagctc ggcctggcgc cgcgccggaa tggcgccgcg
     3541 ccgcacccag ccgctgacgg cggtctgcca gatgccgagc agctcggcga cccgcgcttg
     3601 cgagccgaac ttgcggatga tgtgatcgac ctggttcttg ggcatggctg caacccgact
     3661 ggtggtgttg actaacgtgt tggcgaccgg cggagagatg ttggccggtc agcctgcacc
     3721 gtcgcattgc cacaagctga agggcgagga tcacgctgtc aacatcataa atgctttgac
     3781 ggcgctgcag catggatgtt atcgctcgcc gctgtatctg acgatcgcgt cgttgggtgt
     3841 ttatcgtgtg ttcaagtggt tcgggagatc cggcgggatg ggcgaaacga gtggaatcaa
     3901 cacttttcgt gatgagccgt ggctgttccg cacctatgcc gggcacagct cggccgaggc
     3961 gagcaacgcg ctcttccgca ccaacctggc gcgcggccag accgggctct cggtggcctt
     4021 cgatctgccg acccagaccg gctacgacag cgacgacccc ctggcacaag gcgaggtcgg
     4081 caaggtcggc gtgccgatca cctccctcga cgacatgacg acgctgttcg acggcatccc
     4141 gctcgaccgc atgaacacct cgatgaccat caacgccacc gcaccctggc tgctggcgct
     4201 ctacatcgcc gcggcggagc gccagggcgt cgaccgggcg gtgctgcagg ggacggtgca
     4261 gaacgacatc atcaaggaat acctgtcgcg cggcacctac atcttcccgc ccgccccctc
     4321 gcttcggctg atcggcgatg tcgtcgcctt cacctgccgg gacgtgccga gatggaaccc
     4381 gaccaatgtc tgctcctacc acctgcagga ggccggggcg acgccggtcc aggagctcgc
     4441 cttcgcgctg gccacggcgg tggcggtgct ggatgccatc cgcgccggcg gccaggtgcc
     4501 cgaggcggag ttcccgaagg tggtcggccg catcagcttc ttcgtcaatg ccggtctgca
     4561 cttcgtcacc gagctcgcca agatgcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnggc ctgccccggc cctgggacca gcaatggtcg ctgcgcctgc agcagatcct
     4741 ggccctcgag accgaccttc tggaccatcc ggacatcttc gacggcagca tcgtgatcga
     4801 ggccaaggtt caggagctga tggctgaggc gaaggcggag ctggcgcgga tcgaggacat
     4861 gggcggggcg ctggccgccg tcgactccgg ctacatgaag agccggctgg tcgagagcag
     4921 cgctgcccgg gtccaggcga tcgccgccgg cacgcggcag gtcgtgggcg tcaactgcta
     4981 caccgagacc gccccctcgc cgctcaccac cggcgaggat ggcgggtttc tcaccgtcga
     5041 tcccgccacc gaggcggccc agatccaacg gctgcgggcg catcgtgccc ggcgcgacgc
     5101 cgggacggtc gaagtggccc tggcggagct ggaccgggcc gcgcgcgagg gacagaacgt
     5161 catgccggcc tcgatcgcct gcgcgcatgc cggtgtcacc acgggtgaat gggcgacctg
     5221 cctgcgccgc atcttcggcg agtaccgggc ccccaccggc atcgccggcg ccaagacgga
     5281 tggtttggac gatgcgctgg cgccggtccg cgcccgggtc gaggcgttgg cgagacgcac
     5341 cggccgccgg ctgaagatcc tggtcggcaa gcccggcctc gatggccatt caaatggcgc
     5401 cgagcagatc gcggtggcgg cacgggattc cggcatggac gtggtcttcg agggtattcg
     5461 cctgtcaccc gcacggatcg tcggcacggc gctcgaggag gcggtgcatg tggtcgggct
     5521 ctcgatcctc tcgggctcgc atctgcagct ggtcgaggag gtgatgacgc ggatgcgcaa
     5581 ggccggcatc ggcgacatcc ccgtggtggt cggcggcatc atcccgccgg cggacgccaa
     5641 ggctctcctc gcctgcggcg tcgcccgggt ctacacgccg aaggacttct cgatcccccg
     5701 gatcatggcg gacatcgtgg acattgcagt ctccgccgaa tctcaggcgg cttgaagcgc
     5761 tagctttgag ttgtccgcga cgggcccctg ccgctggtca ccggaatcga gaccagcaag
     5821 caagggcctc atcgatctcg tataccggtt gccgcgcgcc cgattacatc aaccccaagc
     5881 cccgcaggct cgcctggccg tcgagcccga tgatgaaatg atcgtgaacc tggacatcga
     5941 agatttccag ggcggcgaca accttgcggg tcagctcgat atcggggcca gacggcgtcg
     6001 gatcgccgct cggatgattg tgcaccatga cgatcgccga ggcgccgacc tcgagacacc
     6061 gtttggccac ctctcgagga tagagcggaa cgtggtcgat ggtgccgcgt tgctgcacct
     6121 catcgagaat cagcatattc ttgcgattga ggaacaggac gtggaactct tcgtgtccaa
     6181 ggtgtgaaaa ccttgccatg caatacctta gcaccgcttc tgatgacgag atgataggtc
     6241 ggtcgaccac atcgaccttc agcaaccgga ctgcggcggc atgcacgcac ttcagcagcg
     6301 cgatcgccat gtaggtcgcc cgccgattct cggtggggaa ctccatcaaa tgtcgcggat
     6361 tcgcgtgcac gaccccggtc aggttgccga atcgagatat cagcgccgcc gccagctgac
     6421 tttgccgctt gggcctgggg ctggaaaccg ccaggaagat ctcgagcagc tcgctgtccg
     6481 aaaggacctc gaggccgcct tccaggaacc gtcggcgcaa ttttcgccag tgctcccaat
     6541 cctgggcctt ctcgcgaccg agagccgcga gcttgtcgaa cgtggccgtc actggtgcct
     6601 cggccagctc cgcgtggcgc gccctgtgcg tcggataaaa gaccgggccg ccattggctt
     6661 cgtctgcgag cgcgtatcgc ccatcgcaaa tcgccagatc tacgttcatg tcgtgtctcc
     6721 tggtaccgcc tcgaacctct ccgctcttgc cggcgggcgg ttctcattgt cacatgctac
     6781 caattatgac atgaaatcaa ccattagttg aattccgttc ggattcaatg gctccacccg
     6841 gcttcctcat ccccgtgccg cccgaaaccg caggcggaca taatcggctg tccagttgcc
     6901 ggcgctgtcg cacagcgccg gctcgagcag gcggacggcc cgggccagca ggcccgcacg
     6961 ctcgaccgcc ggggcgtggc gcaggaaggg ctgcgcaaag gtctcgagcc agccggccat
     7021 gccggtcggc aagggcgtcg gccgcgggat cagctcgatc gacgagacgc tgaaaccctg
     7081 ccgttcgagc cgggcggcgt gctcgtccgg gctcgggaaa taccagggcc gcatggcgaa
     7141 ggcgtcgatg ccggcctcgc ccagcacggc gcgcagggcg gtggtgatgg cggccacatt
     7201 gcccatgccg ccgaactcgg cgacgaaccg tccgccgggc cgaagggccc tggccacacc
     7261 ggcgatgacg tcgtccggcc gggtcatcca gtgcagcgcc gcattggaaa agacggcgtc
     7321 gaactcggct tcgaacggca aggcgtggcc atcggccagg accacgtcga gacccctcgc
     7381 ccgtgccgca tcgagcagct ctggcgaggc atcgcagccg accacctcgg cgccggtcgc
     7441 caccagctcc gccgtcagcg ccccgtcgcc gcaaccgaga tcgagaatcc gctcaccggc
     7501 cacgggtgcc aggagctcga gcaccggtcg gccgagcgtg gcgacgaaac cggcatggtg
     7561 ccggtagccc gtggcgctcc agctctgtcc ggcggcagca cgatccatca tggccgaatg
     7621 ctatgtctga tccataggga accggcaagc ggtagccacg gcccagcccg acatccagct
     7681 caatggagtc cgatcaagct ctgggccaga tacagtgtct gccctggatg gggataagtc
     7741 cgtgagtgtg gggctccgat cggacttccg tggtgaagca cttcagtcga acggagctag
     7801 tcaggcatct tgcgggacgg acagccgggg acgcagggca ttaaccagct tgtttgcggt
     7861 acattccaac cacgccaatt gctcatccgt accgccgaaa tcggtaagcg ggcgagcgat
     7921 accaatgatg tcgcgctgcc ccggttgacc gaccttgaaa tacagatctc gaagacccgt
     7981 ttccgcacga agcgcttcag cctcgggcgc gagctcggcg catagcatgt tgccttgctc
     8041 gcagcgcaga aagagccctg cttccccctc cccctcactc cgataggccg tcagccagcg
     8101 agctggtggc ggcagtggaa ttcttactga attatttgtt ccatgcctcg gcgtcggctg
     8161 atcgggatgc tcgaaacacg ctgtttcgat gaaacgctgc cagaactctc tattgctctc
     8221 tctgcggatc ctcgcgcctt ccggatccac aacctcttca atttcagttc tgtcgtccga
     8281 cccggcgaga tcgaccaact gaacgggtat gcccgcgtca tccagaatta gccgctggcg
     8341 gatcacctcc gtccgcattg cgacctgcgg caccaccaaa gtccgccccc catcgtcgca
     8401 ccacaactgg agctcgatca gcgcgagtct cgccccctgt gcgccgagat gctccgagat
     8461 gactgtcaga tcctcgcgga tgccttcgcc tgctatgatc agatggaagt cgccggctcg
     8521 gagattgcgc gccaccgcat cgacaaagcg cacctcgtcg gcaggttccg acagtccatg
     8581 gcggacatga gtaaacaggg gattgtcgtc tttcgtgccc agaaccgccc tgagctggac
     8641 ggtgagatcg gcaaaggatc gccgccgtag cagcgccgca tactccagca gttgggccac
     8701 aacttcacgt cgagcctgag ggttgcgcca caatttgcac tccaccagaa cgggacgtcc
     8761 gtgtggtgtt acccccaata tgtccaggaa gacggaccgt ccgcccggct tgggcagcga
     8821 cagctcccgg caaatcggaa ttacctcacc ggcacctggc tcgattcgtt cgaggggaat
     8881 gaggtgcggg tggcgaaaga gtagatcctg gagccatcgt tcatcgtgcc cgttgccgag
     8941 cggagccctc ttcaggcggt tcccggccag accatccgct tccaagaaaa cagcactcat
     9001 cctgtcctcc gctgcaacat cgaaggacgt catagcgctg cgaacagcct ataaccataa
     9061 gttaaattat tatcttcaca atcccctaat tcgcgatttt tccgtcgtgt ttcagggtct
     9121 gcgcgagatt gtgcggtggc taccgccgcg ccaacgccgc gatgcgtagc cggagcgcgt
     9181 tgagcttgat gaagccttcg gcgtccttct ggtcgtagac ggtgtcggcc tcgaaggtgg
     9241 cgtagtcctc gctgtagagg ctctgcggcg ccttgcggcc gaccacggcg gcgctgccct
     9301 tgtagagctt gaggcggacc gtgccggtca ccgggccctg gctctggtcg atcagcgcct
     9361 ggagcatctc gcgctcgggg ctgaaccagt agccgttgta gatcagctcg gcgtagcgcg
     9421 gcatcagctc gtccttgagg tgggcggcac cgcggtcgag ggtgatgctc tcgatgccgc
     9481 gatgggcggc gagcaggatg gtgccgccgg gcgtctcgta gacgccacgg ctcttcatgc
     9541 cgacatagcg gttctcgacg agatcgagcc ggccgatgcc gttcgccccg gcgaggtcgt
     9601 tcaggcgccg cagcaggttg gcgggcgaga gcggctcgcc gtcgaccgag acggcgtcgc
     9661 cccgctcgaa gccgatctcg acataggtcg gccgctcggg cgccagctcg gggctgaccg
     9721 agcgggtgaa catctcctcg tccgcctcga tccagggatc ctcgagcgcc aggccctcgt
     9781 aggagatgtg caggaggttg gcatcggtcg aatagggtgg tgttccccgc ttgccgcggg
     9841 ggatcggaat ctgccggctc tcggcgtact ggatcagctt gtcgcgggag ttcaggtccc
     9901 agagccgcca gggcgcgatg accttgatgt gcgggtcgag cgcatagcag ccgagctcga
     9961 agcggacctg gtcgttgccc ttgcccgtcg ccccgtg
//
LOCUS       DAOTFI010000186         9968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8064_length_9968_cov_6.487945, whole genome shotgun sequence.
ACCESSION   DAOTFI010000186 DAOTFI010000000
VERSION     DAOTFI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_9968_cov_6.487945"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="P5558_17215"
     CDS             <1..463
                     /locus_tag="P5558_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26112.1"
                     /translation="ILAMAIAAALGIGATNTVIAMLVVWWPKFARLARSLVIVQRNQE
                     YVEAAVNIGYGPGRILLRHIMPNSMGPMVVLVTLDVGQAILTFSGLSFLGLGVIPPVP
                     EWGAMVAEGRELIQQWWVAAFPGFAILTVVMGFNFLGDGVRDWLDPRSRTR"
     gene            460..2547
                     /locus_tag="P5558_17220"
     CDS             460..2547
                     /locus_tag="P5558_17220"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26113.1"
                     /translation="MTEPAVNKAAGSTAPLLEVNDLRVHFHTDNGVVRAVDGVSFHVD
                     AGETVGIVGESGSGKSVTALSIMRLLEEPSRIAGGEIVFRGDDVLAMDKERIRSLRGG
                     EVAMVFQDPMTSLNPVIRVARQLVEALLVHRRITPEAARGRAVGLLGRMGITSPDRAI
                     DNFPHEFSGGMRQRVMLAMGFGNEPSLLIADEPTTALDVTIQAQILDLLKELNRDFGT
                     AILMISHDLGVIANVCSRIIVMYGGEVVEEGRIEELLADPRHPYTWALINAVPRLDRD
                     PGADRRLTTIEGTPPDALDMPSGCRFAPRCPFRIAKCDEHPELERIGPRRSTRCWVTQ
                     AGEPLPRRQEHAARAATPSSVGPASRRRPVDGAAPLLELRGLVKHFPLRRRGFFGPQE
                     VIHAVDGVNLTVGLGETVGLVGESGCGKSTLARLITRIHDPTAGEILFEGTDIAQRSR
                     AGMRPLRRRMQMIFQDPFASLNPRMTVGEILAEPIRFHGLAQGRAVNERVQELLALVG
                     LNPRAAGSYPHEFSGGQRQRIGTARALAVDPQLIVADEPISSLDVNIQAQVINLFLDL
                     QQRLGLTFLFIAHDLAVVRHVSDRIVVLYLGKVMETAPAATLFEEPLHPYSVSLISAV
                     PIPDVEAERSRRRILLEGDPPSAIHPPSGCRFRTRCPIAREICAAEVPPLREHRPGHF
                     AACHFPGGMGEWR"
     gene            3148..3411
                     /locus_tag="P5558_17225"
     CDS             3148..3411
                     /locus_tag="P5558_17225"
                     /inference="COORDINATES: protein motif:HMM:NF025304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY26114.1"
                     /translation="MSEGRYRHLTFEEREQIVVWRLEGLSQTAMARRVGRSSSTINRE
                     LARNRLPSGGYQPSFAEGSYPARNPSGDGVASRDPTRPSTSLA"
     gene            complement(3745..4023)
                     /locus_tag="P5558_17230"
     CDS             complement(3745..4023)
                     /locus_tag="P5558_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26115.1"
                     /translation="MATVQRIVDIAMLVPLGPQLTVELECRFSRHRVHAEADPPAYLC
                     QHGDRMRGAVPHGVDGISTTRPHAMPDLETCTIVDVGLETTDVAGCCK"
     gene            complement(4090..5004)
                     /locus_tag="P5558_17235"
     CDS             complement(4090..5004)
                     /locus_tag="P5558_17235"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY26116.1"
                     /translation="MSSFSGVYPILYSFFGADGRLDHEAMQLQVDRCIAAGVHGIAVL
                     GNVTESNKLTQAERVELMELVGESIAGRVPYAVTIGEPSIDGQKALARVAEAACADWL
                     ILQPPAVKGVPESEIVRFFGAVADAASLPIAVQNNPVNMDVWLSNPSLLALHRNHPNV
                     SLLKGEGAASWVQQLIEATGGSLDVFAGLAGIEQIANLESGCVGLIPAPDCVDIQVRI
                     HALWQEATEDSRAEAERLYKEVLPLIVFMTRNLQTHQLAVGKRFVAKRLGLAEVHVRT
                     PAATPTPFQLREVARIGDWLGPLGGERR"
     gene            complement(5001..6017)
                     /locus_tag="P5558_17240"
     CDS             complement(5001..6017)
                     /locus_tag="P5558_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26117.1"
                     /translation="MHVTLTSDFVGPDGRIKLGEEPLARLRAIPGIEISTIAGRPTNH
                     PLEARDVTACDILIGKRNRLDADLLGLPGFRLKHIARMGVGYDHLDLDACTRAGVLVT
                     ITREAVKRPLASAMLALMLALAHRLFERDRLARRADWATRFAIEGVGLSGRTLGLLGA
                     GNIGAELIRLVQPFGMRVLVHDPGLSPDTLAQLGAASGSLDEVVRQADFLMLACSLNE
                     RTKGLINAARLAQMKPTAFLVNLARGELVEEAALVEALACSRVAGAGIDVFEHEPPAR
                     DNPLLGLPNVILGQHNLGMTDELDRLGQMAAVDAVIEVARGNLPASAVNPEAWSQTLQ
                     RFAR"
     gene            6083..6808
                     /locus_tag="P5558_17245"
     CDS             6083..6808
                     /locus_tag="P5558_17245"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY26118.1"
                     /translation="MNGDMPPNRIREANAAGRKAVGFLMNFPSPCIVELLGRQGGFDF
                     AYADGEHGAFDLRDLEEHCRACDLVGLTPIARIPEISRGTVGRFLDRGMRGIIAPHVD
                     TAADARRLVEYCRFHPEGSRSYGAGRAEGLGVGVDLERHCRHWNANVLVGVMIESQTA
                     IDNLDALLAVDGIDCFMIGANDFAQGLGYPGQPAHPAAIAAAAEVAARVRAAGKSMRE
                     DVMRSIWMRDLLLDAAAGFVAQG"
     gene            complement(6799..8478)
                     /locus_tag="P5558_17250"
     CDS             complement(6799..8478)
                     /locus_tag="P5558_17250"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY26119.1"
                     /translation="MSGASEGPARLHIALFSDTHIEPEGDPEPRSNRRAAAIVERING
                     DGFELALHLGDVVHPLPSMASAEAAWQAADAILGRLRMPLAVIPGNHDIGDKSLPWLP
                     AKSVRADWVESFEARFGPACRAIDHGPVRLVLLNAPLLNSGLDGEVGQWRQLEELLAE
                     PGERRLFAFLHYPPFLVAADEPSTYDNLDEPGRARLLALLADHGCEALFCGHVHHRFL
                     DHYAGTAIYTVPATGFTRRDYVETHPLAPTPEQEHGRNLTTKLGFLELAIHEHGHRTR
                     FHRLENDFGPPAGHALGVDLRHPWSEVRALPYNPPTDAFQRRRVRDDGAVLALLDLGI
                     RRARVPLDDFLDPVVRGRMAFLAEQHGVRWTVFALGPLAPDVAGQLAENASMIEAVEV
                     IGESGAEMSGLPVVAAPLITAHAAVGDATNLGQFTGWGLTLEALRALPPDLAPGISGY
                     VLDLPWRQPFAETCEAAAALAGERGWRLHVTVGLMGSRPDLANTDDQAIVRRVRGAAA
                     FAARNTAHRCFLDTFESIDRGYFVRHGLIDRRGDPTPAGLALARFGKEDQP"
     gene            complement(8475..9656)
                     /locus_tag="P5558_17255"
     CDS             complement(8475..9656)
                     /locus_tag="P5558_17255"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26120.1"
                     /translation="MTVPLDFSIRRLDVFHVRAAGGGYWEGYVDGRSRAAERYILANG
                     WRTVYARHVESAVVRVELADGRVGWGEANAPILPEVVCQIARDLLAPLLLGRDFIDPS
                     AMWGFLYDLQRGRGHQGGYQVDAVAAIDIAVWDALARRADVPLAALLGEQPRSMIPVY
                     LSGLRQATLAERVAHAREWAGRGLSGVKLFLDADIEAGCTELAALKEAVPAIDRWMVD
                     LLWSLETLDEAVAAKAAYGAQDVGWLECPLLPEDIDGHRQLHAQPGAPIALGEHFHTS
                     FEVAPWLEPRALDVLQPDIGRTGISNALQQRLMALAAGIDVTPHMGSGLDIFQAATLQ
                     FAAICNDGPLTELQGGLSSRLGDAVDSGWHFADGGFRLPDRPGIGIEVDANRLGALRA
                     S"
     gene            complement(9780..>9968)
                     /locus_tag="P5558_17260"
     CDS             complement(9780..>9968)
                     /locus_tag="P5558_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26121.1"
                     /translation="CYHPLQIDEDRKGLRLQNLLNREAVRDANDFPAVRRFEQATAFF
                     DENRANDDSFLQIETLPG"
BASE COUNT         1611 a         3369 c         3467 g         1521 t
ORIGIN      
        1 catcctggcg atggcgatcg ccgctgccct cggcatcggc gccaccaaca cggtgatcgc
       61 gatgctggtg gtttggtggc ccaaattcgc ccgcctcgcc cgcagcctgg tgatcgtgca
      121 gcgcaatcag gaatacgtgg aggccgcggt caacatcggc tacggccccg gccgcatcct
      181 cctgcgccac atcatgccga attccatggg gcccatggtc gtgctggtga cgctcgatgt
      241 cgggcaggcg atcctgacct tctcgggcct cagctttctc ggcctcggcg tgatcccgcc
      301 cgttccggaa tggggtgcca tggtcgccga gggccgcgag ctcatccagc aatggtgggt
      361 cgccgccttt ccgggcttcg cgatcctgac cgtggtgatg ggcttcaatt tcctcggcga
      421 cggcgtgcgc gactggctcg atccgcggtc ccgcacgcga tgaccgagcc agcggtgaac
      481 aaggcggccg gtagcacggc accgctgctc gaggtcaatg atctccgggt ccatttccat
      541 accgacaacg gcgtcgtgcg ggccgtcgac ggggtgagct tccatgtcga cgcgggcgag
      601 accgtcggca tcgtcggcga atcgggctcc ggcaagagtg tgaccgccct ctcgatcatg
      661 cggctcctcg aggagccgtc gcgcatcgcc ggcggcgaga tcgtctttcg tggcgacgac
      721 gtgctggcga tggacaagga gcggatccgc agcctgcgcg gcggcgaggt cgccatggtc
      781 ttccaggacc cgatgacctc gctcaatccc gtcatcaggg tggcgcggca gctcgtcgag
      841 gcgctgctgg ttcaccgccg catcaccccc gaggcggcgc gcggccgggc ggtgggcctg
      901 ctcggccgga tggggatcac ctcgccggat cgcgccatcg acaatttccc gcacgagttc
      961 tcgggcggca tgcggcagcg cgtcatgctg gcgatgggct tcggcaacga gccgtcgctg
     1021 ctgatcgcgg acgagcccac cacggcgctc gacgtcacga tccaggcgca gatcctcgat
     1081 ctcctgaagg agctgaaccg cgacttcggc accgccatcc tgatgatcag ccacgatctc
     1141 ggcgtcatcg ccaatgtctg ctcgcggatc atcgtgatgt atggcggcga ggtggtcgag
     1201 gaaggaagga tcgaggagct cctcgccgac ccccgccatc cctacacctg ggcgctgatc
     1261 aacgcggtgc cgcgcctcga ccgcgatccg ggggcggacc ggcgcctgac cacgatcgag
     1321 ggcacgccgc ccgacgcgct cgacatgccg tcgggctgcc gcttcgcccc gcgctgcccg
     1381 ttccgcatcg ccaagtgcga cgagcatccc gagctggagc gcatcgggcc tcggcgcagc
     1441 acccgctgct gggtgacgca ggcgggcgag cccctgccgc ggcgccagga gcacgcggct
     1501 cgggcggcga cgccgtcgag cgtcggcccg gcgagcaggc gacggccagt ggacggtgcc
     1561 gcgccgctcc tcgagctccg ggggctggtc aagcatttcc cgctgcggcg ccggggcttc
     1621 ttcggcccgc aggaggtcat tcacgcggtc gatggcgtga acctgacggt cggcctaggc
     1681 gagacggtcg ggctggtcgg cgaatcgggt tgcggcaagt ccaccctggc gcgcctgatc
     1741 acccgcatcc acgacccgac cgccggcgag atcctgttcg agggaacgga catcgcgcag
     1801 cgcagccggg ccgggatgcg gccgctcagg cggcgcatgc agatgatttt ccaggaccct
     1861 ttcgcatcgc tcaacccccg catgaccgtg ggcgagatcc tcgccgagcc gatccgcttc
     1921 cacggcctcg cccagggccg ggcggtcaac gagcgggtcc aggagctcct ggcgctcgtc
     1981 ggcctcaacc cgcgcgccgc cggcagctac ccgcacgagt tctcgggcgg ccagcgccag
     2041 cgcatcggca cggcgcgcgc cctggcggtc gacccgcagc tcatcgtggc cgacgagccg
     2101 atctcgtcct tggacgtcaa catccaggcc caggtcatca acctcttcct cgacctgcag
     2161 cagcggctcg gcctcacctt cctcttcatc gcccacgacc tcgccgtggt ccgtcacgtc
     2221 tccgaccgga tcgtggtcct ctatctcggc aaggtcatgg agaccgcccc ggccgccacc
     2281 ctgttcgagg aaccgctcca tccctattcc gtgtcgctga tctcggcggt gccgataccg
     2341 gatgtcgaag ccgagcggtc gcgtcgacgc atcctgctcg agggggatcc gccgagcgcc
     2401 atccacccgc cgagcggctg ccgcttccgc acgcgctgcc ccatcgcccg cgagatctgc
     2461 gccgccgagg tgccgccgct ccgggagcat cgccccggcc acttcgccgc ctgccacttt
     2521 cccggcggca tgggcgagtg gcggtgaggg cgatctggag gttgccggcc atggccagca
     2581 gtgccgtgac gccgatgccg gcgatccagc cggccgtgct gctggtagcc accaaggctg
     2641 gacgatcgtg ttcggggggc cggttggcga gataggtcaa gcgcccctgg atcgagccct
     2701 cctcgtccca cgtcagcaag atgaagaacg cggcgcagaa gtaaggcacc tgccatgggc
     2761 cggtcgcctc gaaaacaaag gtcatggtac gtgccagtgc ggcgagcagt gcgccggccg
     2821 cgattcggtg gcgggtcggg acctttgccc agaacccgct gagcaggacg cagacgctgt
     2881 tggtcagcac gaacagggtg acgttctggg cggccggatc gtgcagggtg gcggcgtgga
     2941 cggcgtagaa cgggagggcc agctcgccgc tcagcaggag cacgttggcg agcagcaggc
     3001 acccatccag ggacaagccc ttatcggctg cagcccgtgc agcaactcgc cccaggcgac
     3061 gaaacacgaa ccctccccga gctcgggtgg attcccgctt caagcgcaga ggtgccaaga
     3121 tgctgaaaac gcttggaggg cgggaggatg agcgagggcc gataccgtca tctgacgttt
     3181 gaggagcgcg agcagatcgt ggtctggcgt ctcgaggggc tgagccagac cgcgatggcg
     3241 cggcgggtgg ggcggtcgtc gtcgacgatc aatcgcgagc tggcgcgcaa tcggctgccg
     3301 tcgggcggct accagccgag cttcgcggaa gggtcgtatc cggcccgaaa ccccagcggc
     3361 gatggagtgg ccagccgaga cccaacccga ccttcaacca gtttggcctg atctatacaa
     3421 tgaccggcgg cggtccgggc aacgctaccc gccactatgg catcctcgtc gacgagaagt
     3481 ccatcggctc cctgcaacat ggtccgggtg cggcactcgc gctgagtgtg gcgcccctga
     3541 tggcggtcct gacatggccg ctcgcgaggt tcatgcggcc tgatgaccac cgtgcgggga
     3601 cggaccggac gccggcgatc gcgccattgc cggcgcgctc acaacggtcg ccctccgcga
     3661 tgcggcggca ggcggccaga gcgtgcgcca gagtgcggtc ggcgaatcgt cgcgggggat
     3721 ggcagtgatg gtgagcacca cgcctcactt gcagcaaccg gcgacgtcgg tggtctcgag
     3781 gccgacgtcc acgatggtgc aggtctcgag atcgggcatg gcgtgaggcc gggtggtgga
     3841 gatgccgtcc acgccgtgag ggaccgcacc gcgcatgcgg tcgccatgct ggcacagata
     3901 ggctggagga tcggcctcgg catggacacg gtggcggctg aagcggcact cgagctccac
     3961 ggtgagctgg gggcccagcg ggacgagcat ggcgatatcg acgatccgct gaacagtggc
     4021 catggcgacg ttttgttcag gcgaggcgct ggttgaccag gcagtccagc aaccatgggt
     4081 ggaaacgctt catcggcgct cgccgcccag cggacccagc cagtcgccga tgcgggcgac
     4141 ctccctgagc tggaacggcg tcggcgtcgc ggccggcgtt cggacatgca cctccgcgag
     4201 gccgagccgt ttcgccacga agcgcttgcc aacagcgagc tggtgggtct gcaggttgcg
     4261 ggtcatgaag acgatcagcg gcaagacctc cttgtagagc cgttccgcct ccgcccggga
     4321 gtcctcggtc gcttcctgcc agagcgcgtg gatgcgcacc tggatatcga cgcagtcggg
     4381 ggccgggatc aggccgacgc agccgctctc gaggttggcg atctgctcga tgccggcgag
     4441 cccggcgaag acgtcgaggc tgccgcccgt cgcctcgatc aactgctgca cccaggacgc
     4501 ggcaccctcg cccttgagaa gcgagacgtt cggatggttg cggtgcagtg ccagcaggct
     4561 cgggttcgag agccagacat ccatgttgac cgggttgttc tggacagcaa tcggcagcga
     4621 cgcggcatcg gcgaccgcac cgaagaagcg cacgatctcg ctttccggca cccccttcac
     4681 ggctggcggc tggaggatga gccagtcggc gcaggccgcc tcggcgacgc gtgccagggc
     4741 cttctgacca tcgatgctcg gttcgccgat ggtgaccgca tagggcacgc ggccggcgat
     4801 ggactcgccc accagctcca tcagctccac ccgctcggcc tgagtcaact tgttgctctc
     4861 ggtgacgttg ccgagcacag cgatgccgtg caccccggcg gcaatgcagc gatcgacctg
     4921 cagttgcatt gcctcgtggt cgagacgacc atcggcgccg aagaagctgt agagaatggg
     4981 atagacacca ctgaagctgc tcaacgtgcg aatctctgca aggtttggga ccacgcctcg
     5041 gggttcacgg ccgatgccgg caggttgccc cgggccacct cgatcaccgc atcaaccgcc
     5101 gccatctgcc cgagccggtc gagttcgtcg gtcatgccca aattgtgttg gccgaggatc
     5161 acgttgggca gaccgagcag gggattgtcc ctggccggcg gctcgtgctc gaagacgtcg
     5221 atgccggcac cggcaacgcg gctacaggcg agcgcttcca ccagcgccgc ctcctcgacc
     5281 agctcgcccc gcgccagatt gacgagaaag gccgtgggct tcatctgcgc cagccgtgcc
     5341 gcgttgatca ggcccttcgt tcgttcgttg agcgagcagg cgagcatgag gaaatcggcc
     5401 tgccgcacga cctcgtcgag cgagcccgat gccgcgccga gctgcgccaa tgtgtcgggc
     5461 gagagccccg gatcgtgcac caggacccgc atgccgaaag gttgcaccag gcggatcagc
     5521 tcggcgccga tattgccggc accgagcagg ccgagcgtgc gcccgctgag gccgacgccc
     5581 tcgatcgcaa agcgcgtagc ccagtcggcg cggcgggcga gtcggtcacg ctcgaagagg
     5641 cgatgggcga gagcgagcat caacgccagc atcgccgagg cgagggggcg cttgacggct
     5701 tcgcgggtga tggtcaccag cacgccggcc ctggtgcagg cgtcgaggtc caggtgatca
     5761 tagccgacgc ccatccgggc gatgtgtttg agccgaaagc caggcaggcc gaggaggtcg
     5821 gcatcgagcc ggttgcgctt gccgatcaga atgtcacagg cggtcacgtc cctggcctcg
     5881 agcggatggt tggtcggccg gccggcgatg gtcgagatct cgatgcccgg gatcgcccgc
     5941 agccgggcca gcggctcctc tcccagcttg atgcgcccgt ccgggcccac gaagtccgac
     6001 gtcagtgtca catgcatggc ctgcctcccg ggtttgccct aggcttcgcc agtagtccaa
     6061 gagcatcaat gaagaggcgc cgatgaacgg tgacatgccc cccaaccgga tcagggaagc
     6121 gaacgccgcc gggcgcaagg cggtgggatt tttgatgaat ttcccgtcgc cgtgcatcgt
     6181 cgagctgctc gggcgccagg gcggcttcga cttcgcctat gccgatggcg agcatggcgc
     6241 cttcgatctg cgcgatctcg aggagcattg ccgcgcctgc gacctggtcg gcctgacgcc
     6301 gatcgcccgc atcccggaaa taagccgcgg cacggtcggg cgcttcctcg atcgcggcat
     6361 gcggggcatc atcgctcccc atgtcgacac ggcggcggat gcgcgccggc tggtcgagta
     6421 ttgccgcttc catcccgagg gcagccgctc ctatggcgcc ggccgcgccg aaggcctggg
     6481 tgtcggcgtc gatctcgagc gacattgtcg ccactggaac gccaatgtgc tcgtcggcgt
     6541 aatgatcgag agccagaccg ccatcgacaa tctcgatgcg ctgctggcgg tcgacgggat
     6601 cgactgcttc atgatcggcg ccaacgactt cgcgcaaggc ctgggatacc cgggccagcc
     6661 ggctcatcct gccgccatcg ccgcggcggc ggaggtcgcg gcacgggtgc gcgccgccgg
     6721 caagtccatg cgcgaggacg tcatgcgctc gatctggatg cgcgatctcc tgctcgatgc
     6781 cgcggcgggc tttgtcgctc aaggctgatc ctccttgccg aaacgggcca gcgccaggcc
     6841 cgccggagtc ggatcaccgc gacggtcgat caggccgtgc cggacgaaat agccgcggtc
     6901 gatcgattcg aacgtgtcga gaaagcagcg atgtgccgtg ttgcgggcag caaacgctgc
     6961 cgctccgcgg acccgcctga caatcgcctg atcatcggtg tttgcgaggt ccggcctcga
     7021 ccccatcaac ccgaccgtca catgcagccg ccagccccgc tcgccggcga gcgccgccgc
     7081 tgcctcgcaa gtttcggcga aaggctgacg ccacggcagg tcgaggacgt agcccgagat
     7141 accaggcgcg aggtccgggg gcagggcgcg gagcgcttcg agcgtgagcc cccaaccggt
     7201 gaactggccg aggttggtcg cgtcgccgac cgccgcgtgc gcggtgatca gcggggcggc
     7261 caccaccggc agcccggaca tttctgcccc gctttcgccg atgacctcca cagcctcgat
     7321 catcgaggcg ttttcggcga gctggccagc gacgtccggt gccagtgggc cgagcgcgaa
     7381 gacggtccag cgaacaccgt gctgctcggc gaggaatgcc atccggccac gcaccacggg
     7441 atcgaggaaa tcatccagcg gcacccgggc ccggcggata ccgagatcga ggagtgcgag
     7501 cacggcgccg tcgtcgcgca cgcggcgacg ctggaaggca tcggtgggcg ggttgtaggg
     7561 cagggcgcgg acctccgacc acggatgacg gaggtcgacg ccgagcgcat ggccagcagg
     7621 tgggccgaaa tcgttctcca gccggtggaa ccgggtgcgg tgaccgtgct cgtggatggc
     7681 gagctccagg aagccgagct tggtcgtgag attgcggccg tgctcctgct ctggtgtggg
     7741 ggccagcgga tgcgtctcca catagtcgcg acgggtaaag ccggtcgccg gcaccgtgta
     7801 gatcgccgtg ccggcataat ggtcgaggaa gcggtgatgg acgtggccac agaagagggc
     7861 ctcgcagcca tgatcggcga gcaaggccag caatcgagcg cgccccggct cgtcgagatt
     7921 atcgtaggtc gagggctcgt cggcggcgac cagaaaaggc gggtagtgga ggaaggcgaa
     7981 aagacgccgc tcgcctggct cggccagaag ctcttccagc tgccgccact gcccgacctc
     8041 gccatcgagg ccgctattga gcaagggggc gttgagcagg acgaggcgga ccggtccgtg
     8101 gtcgatggcg cggcacgccg ggccgaaacg cgcctcgaag ctctcgaccc aatcggcgcg
     8161 caccgacttc gccggcagcc acggcaagga cttgtcgccg atgtcgtggt tgcccgggat
     8221 gaccgcgagc ggcatcctca gtcgcccgag gatggcgtcc gccgcctgcc acgcggcttc
     8281 ggcgctcgcc atcgacggta gcgggtgcac gacatcgccg agatgaagtg cgagctcgaa
     8341 gccgtcgcca ttgatgcgct cgacaatcgc cgccgcgcgc cggttcgagc gcggctcagg
     8401 atcaccctcg ggctcgatat gggtgtcgct gaagagtgcg atgtgcagtc ttgcgggacc
     8461 ttccgacgcg ccgctcacga tgcgcgcagt gccccgagcc ggttggcatc gacctcgata
     8521 ccgatgccgg gccggtcggg caggcgaaac ccgccatcgg caaagtgcca gccgctgtcg
     8581 accgcatcgc cgagccgcga gctcaggcca ccctgcagct cggtcagcgg cccgtcattg
     8641 cagatcgccg cgaactgcaa tgtcgccgcc tggaagatat ccaggccgga gcccatgtgg
     8701 ggcgtgacgt cgatgccggc ggccagcgcc atcaggcgct gctgcagcgc attgctgatg
     8761 ccggtgcggc cgatatcggg ctgcaggacg tcgagggccc gcggctcgag ccagggcgcc
     8821 acctcgaagg aggtgtggaa atgctcgcca agggcgatcg gcgctccggg ctgtgcgtgc
     8881 aactgccgat ggccgtcgat gtcctccggc agcaagggac actcgagcca gccgacgtcc
     8941 tgggcgccat aggccgcctt ggcggcgacg gcttcgtcga gcgtctcgag gctccagagg
     9001 agatcaacca tccagcggtc gatcgccggc actgcctcct tgagcgctgc cagctccgtg
     9061 cagcccgcct cgatatcggc atcgaggaag agcttgaccc cggagaggcc gcgaccggcc
     9121 cattcacggg cgtgcgccac ccgctccgcc agggtcgcct ggcgcagacc cgacaggtag
     9181 accgggatca tggaccgggg ttgctcgccg aggagtgccg ccaacggcac atcggcacgg
     9241 cgggcgagcg catcccagac ggcgatgtcg atggcggcca cggcatcgac ctgatagcca
     9301 ccctggtggc cgcggccgcg ctgcaggtca tagaggaagc cccacatcgc cgaggggtcg
     9361 atgaagtcgc ggccgagcag caggggcgcg agcaggtcgc gcgcgatctg gcagaccacc
     9421 tccggcagga tcggcgcgtt ggcctcgccc cagccgacac gaccatcggc gagctcgaca
     9481 cgcaccaccg cgctctcgac atggcgcgca tagaccgtgc gccagccatt ggcgaggata
     9541 tagcgctccg ccgcccggct gcggccgtcg acatagcctt cccaatagcc gccgcccgcg
     9601 gcgcgcacgt gaaagacgtc gaggcgccgg atgctgaaat cgagcggcac cgtcatggcg
     9661 ccagggccag tcgcgcatag atctcgggcg gcgccgcgtg ggcgcccatc tcgtccagca
     9721 tgagggcgac gagccgctcg gttgcggctt cgtcctggac cgggcgcatc tggcccggat
     9781 cacccaggca aggtctcgat ctgcaggaat gagtcgtcat tggcgcgatt ctcgtcgaaa
     9841 aacgcggtgg cttgctcgaa gcggcgcacg gccggaaaat cattcgcgtc gcggaccgcc
     9901 tcgcgattga ggaggttctg caaccggaga cccttgcggt cctcgtcgat ctgcagcgga
     9961 tgatagca
//
LOCUS       DAOTFI010000187         9909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8124_length_9909_cov_7.265070, whole genome shotgun sequence.
ACCESSION   DAOTFI010000187 DAOTFI010000000
VERSION     DAOTFI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8124_length_9909_cov_7.265070"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..599)
                     /locus_tag="P5558_17265"
     CDS             complement(99..599)
                     /locus_tag="P5558_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26122.1"
                     /translation="MGNALKWLAQGLVLALIMATIGYLATRPAYTNFPEDMALIRLSF
                     SHGGGRPDCRRRTPEELAALPPNMRAPMDCPRGRLPVLVELDLDGGPLFHALLPPGGL
                     QGDGPSRVHRGFAVPPGTHTITARLRDSDRVDGFDFEKEITLDLVPRQNLVIDFKTDA
                     GGFVVH"
     gene            complement(592..2169)
                     /locus_tag="P5558_17270"
     CDS             complement(592..2169)
                     /locus_tag="P5558_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRY26123.1"
                     /translation="MDLLRRTLRAGFARLEALLDRVFPPAWQPLYHLGALSFFCLWIV
                     FVSGLYVYAFFDTGTVDAFESVENMSTSYWYHAGVMRSLHRYASDGMVLFMLLHLLRE
                     FSLDRYRGSRWFTWVTGVVIIWLVYISGITGYWLVWDELAQFVAIRSSELVDAIGIFG
                     EPIARNFLTPGSLDDRFFSLMLFLHIAIPLILLMVMWIHLQRVTRPEINPARGLAILM
                     LVAFVALALALPATSQAPADLGLVLGRIGLDWYYLGTYPLIDLIGARGLLGLLGVITV
                     ILVALPLMPPMRRPPAATVNLEFCNGCERCVHDCPYEAVKLVPRTDGLPFQHEARVDP
                     SLCVSCGICTGACPTATPFRQRGRMVAGIELPHLALKDLRALTDKAALGLTGDRRVIA
                     IGCDHGVELKGLGDPAVGVVRLPCTGMLPPSFIDYILARDLADGVVLTGCAEEACQNR
                     NGIAWTEARIEGRRDPYLRQRVPRERILRVWPGKTGTKALARAIEAFRATLPEQPSAK
                     AAKRQPAPAGPAVTGDG"
     gene            complement(2175..3017)
                     /locus_tag="P5558_17275"
     CDS             complement(2175..3017)
                     /locus_tag="P5558_17275"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRY26124.1"
                     /translation="MQRYSARSPIGAVLLCLGLLGPAAADEAPNPFDERAALAYSQAA
                     IGRSVSPHVFLDDAGQRVSLGDFRGRPLVLNLVFTACSTTCPVIVQSLHRSVQIANGA
                     LGPDAFSVVTIGFDTDRDTPEQMRAFRASQGVRMPNWSFLSADEATIEALSAEVGFIY
                     FPSPRGFDHLTTTTILDAEGTVFRHVYGDAFEPPAVVEPLKDLVWGRFSPVADLEGAW
                     NRIRLLCTLYDPKNDRYRFDYGIFIGLIAGIICLGGIAVVLARAWLATRRRPDPASAL
                     PARR"
     gene            complement(2998..3873)
                     /gene="cyoE"
                     /locus_tag="P5558_17280"
     CDS             complement(2998..3873)
                     /gene="cyoE"
                     /locus_tag="P5558_17280"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRY26125.1"
                     /translation="MRPTQPLVQHIVSVFKLRIAVVIALSALAGVAVAPGAPLSPAQI
                     LVLTVAVLLSSGAAGAFNQLFERDLDALMVRTRRRPFASGEWRGGPLWWALILAILAL
                     GVGMAGLVLNAATALYLFLGAFTYGVVYTVWLKRRTVWNIVIGGLAGSFAVLAGAAAV
                     DPSPSAAPLVLAVVLFLWTPPHFWSLALMAKDDYAANGVPMLPAVKGDAFTAWAILVH
                     AIALAVLAVVPFFLGMGWIYLAAAVVGGAGFVRTSLALVRRPSRATAFANFKSSLLQL
                     SLLLVGAMGDAALLG"
     gene            complement(3880..5598)
                     /locus_tag="P5558_17285"
     CDS             complement(3880..5598)
                     /locus_tag="P5558_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRY26126.1"
                     /translation="MASIAVPGRQVVEERFRTCPDTGLEVHAKAETLIRVNVLTAVAF
                     LAVGGLFGLLIALTRWQAIHLLPAEWFYLVLTAHGAAVLIFWIMFFEVALFYFTSAVV
                     LNSRLATPMMAWVAYVLMLVGALVATVAVLQGNSSVMFTSYPPLQAAPHFYLGLILFA
                     VGTLISCFIFLGTLVVAKDEGTYEGSIPLATFGGLTAVIIMIFTLASGAIILIPTFLW
                     SLGIIGTIDPLTYKLVWWGLGHSSQQVNVTLHVVVWYTIAALVLGARPLSEKVSRTAF
                     LLYILFLQLASAHHLLVEPGLSSSWKIFNTSYAMYLAVLGSMIHGMSVPGAVEAAQRR
                     LGHTKGIFGWLREAPWGNPAFAGMFLSLVMFGFLGGISGVILGTEQINILMHNTLYVP
                     GHFHATVVCGTTLAFMAVTYLLIPIFFQRDLVFPRMAKWQPYVFGAGAAGISLFMMGA
                     GTLGVARRHWDITFTDALISFDYPATAFFMLGLNGLSALVAAAGGVMYVLVVFGSVFW
                     GKRRTAAEAKTHPIAPGAIALRTYGSEGTLKLPGTIVLVAVFFIAFALYYFINWRFLA
                     GIWGMN"
     gene            complement(5601..6182)
                     /locus_tag="P5558_17290"
     CDS             complement(5601..6182)
                     /locus_tag="P5558_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HRY26127.1"
                     /translation="MALLPPKNRLWWNEPLHRMEIVWITIAFLWGIAMFSTMIWWHIY
                     GEQNLSNESYRILPEVYAERVEAMIEEYQVGEDEFGMPIVRPPVGADVYLQARLWDWS
                     PILELVEGETYRLHISSLDWQHGFSLQPTNINLQVHPGYEMVVTLTPNESGEFGIVCN
                     EFCGIGHHLMTGRINVLDREAAKAAGMQLAAGE"
     gene            complement(6200..6367)
                     /locus_tag="P5558_17295"
     CDS             complement(6200..6367)
                     /locus_tag="P5558_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26128.1"
                     /translation="MARQRTEAEEGGRPHVMQRLLDNPFLLVFIGVTMPTVLYLIWGI
                     MEIAQIPVAPS"
     gene            complement(6542..7054)
                     /locus_tag="P5558_17300"
     CDS             complement(6542..7054)
                     /locus_tag="P5558_17300"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY26129.1"
                     /translation="MEPRHWRDNSLQSPKRRSNAVLALALVGGLAAMAPTSPAHADDA
                     IGPLLPPNVRAMLLEEMAAVLGATQMILDALVRGEDAVVAEKAQAIHDSFILEQQMTD
                     ADMQALLAVAPAEFVELDRAFHELAASLAEAARAGEQARQRELFGEVVEACAGCHARY
                     AANLFPGFTR"
     gene            complement(7127..7309)
                     /locus_tag="P5558_17305"
     CDS             complement(7127..7309)
                     /locus_tag="P5558_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26130.1"
                     /translation="MEIVLFTVVAGTLYLAADRLLALVERRRGGPLPNRSVIFFLLLL
                     GLALPVFALIRSFLGT"
     gene            7520..8245
                     /locus_tag="P5558_17310"
     CDS             7520..8245
                     /locus_tag="P5558_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY26131.1"
                     /translation="MRGLAAADAVRLARLPMFADLAQAKRAEIIEEASVVAVSRRTVL
                     FYQGEPATRFYVVLDGCVMLHRVSPNGHQSVISLINRGESFAEAAMFDCADFPVTATV
                     IADARLLVVTAQSFRRRLMSDPSLALNILASMSRQMRRLVEQIEQRSSSSSTERLARF
                     LLQLCPEDVAATELELPLDKQLIAGALGMQPETFSRCLSRLKPLGVRAHGNHVEIADI
                     AALQRLCSGDGTPRMRPGGPGRR"
     gene            complement(8250..8792)
                     /locus_tag="P5558_17315"
     CDS             complement(8250..8792)
                     /locus_tag="P5558_17315"
                     /inference="COORDINATES: protein motif:HMM:NF025592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4334 domain-containing protein"
                     /protein_id="HRY26132.1"
                     /translation="MPAAAPGWLLAWRARAIDPAAALDRFDALPGLEPEAMLGSWRGC
                     SLPTGHPLDGLLEVLGWQGKEFEAVDRVHPLLFRGPLGVPVALEPAWLPVGLALRLPG
                     FSRSRPVRAAFRALRPLLRARGPAAKLETRVFRGRSSAAMVYDRQPITDHFRRIDADR
                     VLGLMELRRSPRPFFFLLRR"
     gene            complement(8797..9837)
                     /locus_tag="P5558_17320"
     CDS             complement(8797..9837)
                     /locus_tag="P5558_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HRY26133.1"
                     /translation="MQTRVCRLHAQGDIRIESIDVAAPGPGEVRLRMGAGGICGSDLH
                     YYQHGGFGPIRVQEPIILGHEAAGTVEAVGPGVDLAIGTKVAVNPSRPCGECGYCQEG
                     KPQHCLQMRFRGSAMRFPHEQGLFRERLVVEAAQCVPVGAGTSLAKAACAEPLAVCLH
                     ARSRAGDLRGRRVLVTGAGPIGSLCVAAAATGGAAEIVVTDLHDATLAAAMAMGATRA
                     VNLSREAGAMDEFKADKGCFDVTFECSAAAPAIRTAIETTRPMGRIVQVGVTGDVAVP
                     LNLIVSKEIELVGTHRFHPEFALAVRLIDEGRIDPTPILTGDFPIEDARDAFELAGDR
                     SRAVKVQLTLAS"
BASE COUNT         1674 a         3249 c         3658 g         1328 t
ORIGIN      
        1 ggcgagaagc ggcaggagca gcccgcgccc ggcgccggtc agcgggccgt atgatccggt
       61 atgctggcgc atcgccgtct tcccttcctg ctggccgctc aatgcaccac gaaaccgccg
      121 gcatccgtct tgaagtcgat gaccagattc tgccggggca cgaggtcgag cgtgatttcc
      181 ttctcgaagt cgaagccgtc cacccggtcg ctgtcgcgca gccgtgccgt gatggtgtgc
      241 gtgcccggcg gcacggcgaa gccccggtgc acgcgggacg gcccgtcgcc ctgcaggccg
      301 ccgggtggca ggagcgcgtg gaagagcggc ccgccgtcga ggtcgagctc gaccagcacc
      361 ggcagccggc cgcgcgggca atccatgggc gcgcgcatgt tgggcggcag ggccgccagc
      421 tcctcgggcg tgcgccggcg gcagtccggc cggccgccgc catggctgaa gctgaggcgg
      481 atcagcgcca tgtcctcggg gaagttggtg taggccggcc gggtggcgag atagccgatc
      541 gttgccatga tcagcgccag gacgaggccc tgggcgagcc atttcagggc attacccatc
      601 gccggtcacc gccggcccgg ccggcgccgg ttggcgcttg gcagccttgg cgctcggctg
      661 ctccggcaag gtggcacgga aggcctcgat ggcccgggcc agggccttcg tgcccgtctt
      721 gccgggccag acgcgcagga tgcgctcgcg cggcacgcgc tggcgcagat aggggtcgcg
      781 ccggccctcg atccgcgcct ccgtccaggc gatgccgttg cggttctggc aggcttcctc
      841 cgcgcagccg gtgaggacca cgccgtcggc gaggtcacgg gcgaggatgt agtcgatgaa
      901 ggacggcggc agcatgccgg tgcagggcag gcggacgacg ccgacggccg ggtcgccgag
      961 ccccttcagc tcgacaccgt ggtcgcagcc gatggcgatg acgcgccggt cgccggtgag
     1021 gcccagggcc gccttgtcgg tcagggcgcg cagatccttc agcgccagat gcggcagctc
     1081 gatgccggcg accatccggc cgcgctggcg gaagggcgtg gcggtcgggc aggcgccggt
     1141 gcagatgccg cagctcacgc agagcgaggg gtcgaccctg gcctcgtgct ggaagggcag
     1201 gccgtcggtg cgcggcacca gcttgacggc ctcgtagggg cagtcgtgga cgcagcgctc
     1261 gcagccattg cagaactcga gattgaccgt ggccgcgggc ggtcgccgca tgggcggcat
     1321 cagcggcagg gcgacgagga tgacggtgat caccccgagc aggccgagca gcccccgcgc
     1381 accgatcagg tcgatgagcg ggtaggtgcc gagatagtac cagtcgaggc cgatccggcc
     1441 cagcaccagc ccgaggtcgg ccggggcctg gctggtcgcg ggcagggcca gcgccaaggc
     1501 gacgaaggcg accagcatga gaatggcgag gccgcgcgcc gggttgatct cgggccgggt
     1561 cacccgctgc aggtggatcc acatgaccat caggaggatc agcgggatgg cgatgtgcag
     1621 gaacagcatc agcgagaaga aacggtcgtc gaggctgccg ggcgtcagga aattgcgggc
     1681 gatgggctcg ccgaagatgc cgatggcgtc gaccagctcg gagctgcgga tcgcgacgaa
     1741 ctgggcgagc tcgtcccaga ccagccagta gccggtgatg ccggagatgt agacgagcca
     1801 gatgatgacc acgccggtga cccaggtgaa ccagcgggaa ccgcggtagc ggtcgaggga
     1861 gaactcgcgc agcagatgca gcagcatgaa gagcaccatg ccgtccgagg cgtagcggtg
     1921 caggctgcgc atcaccccgg catggtacca gtagctggtg ctcatgttct cgaccgactc
     1981 gaaggcgtcc accgtgccgg tgtcgaagaa ggcgtagacg tagaggccgc tgacgaagac
     2041 gatccagagg cagaagaagg agagggcgcc caggtggtag agcggctgcc aggcgggcgg
     2101 gaagacccga tccagcaacg cctcgaggcg ggcgaagccc gcgcgcaagg tgcgacgcag
     2161 cagatccatg cccgctaccg cctcgccggc agggccgacg ccgggtccgg ccggcggcgg
     2221 gtggcgagcc aggcgcgcgc cagcacgacg gcgatgccgc cgaggcagat gatgccggcg
     2281 atcagcccga tgaagatgcc gtagtcgaac cggtagcggt cgttcttcgg gtcgtagagg
     2341 gtgcagagca gcctgatccg gttccaggca ccctcgaggt cggcgaccgg gctgaaccgg
     2401 ccccagacca ggtccttcaa gggctcgacc acggccggcg gctcgaaggc gtcaccatag
     2461 acatggcgga agaccgtgcc ctcggcatcc aggatggtgg tcgtggtcag gtgatcgaag
     2521 ccgcgcggcg acgggaagta gatgaagccg acctcggccg aaagagcctc gatggtggcc
     2581 tcgtcggcgc tgaggaagga ccagttcggc atgcgcacgc cctggctggc gcgaaacgcg
     2641 cgcatctgct cgggcgtgtc gcgatccgtg tcgaagccga tcgtgacgac cgagaaggcg
     2701 tcgggcccga gcgccccgtt ggcgatctgg acactgcggt gcagcgactg gacgatgacc
     2761 gggcaggtcg tggagcaggc ggtgaagacg aggttcagaa cgagcggtcg gccgcgaaaa
     2821 tcgccgaggc tcaccctttg gccggcgtcg tcgaggaaga cgtggggcga gacgctgcgg
     2881 ccgatggcag cctggctgta ggcgagggcg gcccgctcgt cgaagggatt gggtgcctcg
     2941 tcggccgcgg ccgggccaag gaggccgagg cagagcagca cggcgccgat cgggctccta
     3001 gccgagtagc gctgcatcgc ccatggcgcc gacgaggagg agcgagagct gcagcagcga
     3061 gctcttgaaa ttggcgaagg cggtggcccg gctcggccgt cggaccaagg ccaggctggt
     3121 cctgacgaag ccggcaccgc cgaccacggc ggcggcgagg tagatccagc ccatgccgag
     3181 gaagaagggc accacggcga gcacggccag cgcgatggcg tgaacgagga tggcccaggc
     3241 ggtgaaggcg tcgcccttga ccgccggcag catcggcacg ccgttggcgg cgtagtcgtc
     3301 cttcgccatc agcgccaggg accagaaatg cggcggcgtc cagaggaaga gcacgacggc
     3361 gaggacgagc ggggcggcgc tcgggctcgg gtcgacggcg gcggccccgg cgagcaccgc
     3421 gaagctgccg gcgagcccgc cgatcacgat gttccacacc gtgcgccgct tcagccagac
     3481 cgtgtagacg acgccgtagg tgaaggcgcc gaggaagaga tagagggcgg tcgcggcatt
     3541 gaggacgagg cccgccatgc cgacgcccag ggccaggatg gccaggatca gcgcccacca
     3601 gagggggccg ccccgccact cgccgctggc gaagggccgc cttcgggtgc gaaccatcag
     3661 cgcgtcgaga tcgcgctcga agagctggtt gaaggccccg gcggcacccg aggaaagcag
     3721 caccgccacg gtcagcacga ggatctgcgc cggcgagagc ggggcacccg gggcgaccgc
     3781 cacgcccgcc agggcgctga gggcgatgac gaccgcgatc cggagcttga agaccgagac
     3841 gatgtgctgg acgaggggct gggtcggccg catggcgggt cagttcatgc cccagatgcc
     3901 ggccaggaac cgccagttga tgaagtagta gagggcaaag gcgatgaaga agaccgcgac
     3961 cagcacgatg gtgcccggca gcttcagcgt gccctcgctg ccataggtcc gcaaggcgat
     4021 ggcgcccggg gcgatggggt gggtcttcgc ctcggccgcg gtccggcgct tgccccagaa
     4081 aacggagccg aagaccacga gcacgtacat cacgccgccg gccgccgcga ccaaggccga
     4141 aaggccgttc aggccgagca tgaagaacgc cgtggccggg tagtcgaacg agatgagcgc
     4201 gtcggtgaag gtgatgtccc agtggcgcct ggcgacgccg agcgtgcccg cacccatcat
     4261 gaagagcgag atcccggcag ccccggcgcc gaacacgtac ggctgccact tcgccatccg
     4321 ggggaagacg aggtcacgct ggaagaagat cgggatcagg aggtaggtga cggccatgaa
     4381 ggccagcgtg gtgccgcaga ccaccgtggc gtggaaatgg cccggaacgt agagcgtgtt
     4441 gtgcatcagg atgttgatct gctcggtgcc gaggatgacg ccgctgatgc cgccgaggaa
     4501 gccgaacatg acgagcgaga ggaacatgcc ggcgaaggcc gggttgcccc agggggcctc
     4561 cctgagccag ccgaagatgc ccttggtgtg gccgagccgg cgctgcgccg cctcgacggc
     4621 gcccggcacg ctcatgccgt ggatcatcga gcccagcacc gccaggtaca tggcgtagct
     4681 ggtgttgaag atcttccagg acgagctcag cccgggctcg accaggagat ggtgggccga
     4741 ggcgagctgc aggaagagga tgtagaggag gaaggcggtc cggctgacct tctccgagag
     4801 cggccgggca cccagcacca gggcggcgat ggtgtaccag acgaccacgt gcagcgtgac
     4861 gttgacctgc tgcgatgagt ggccgaggcc ccaccagacg agcttgtagg tcaggggatc
     4921 gatggtgccg atgatgccga gcgaccagag gaaggtcggg atcaggatga tcgccccgga
     4981 ggccagggtg aagatcatga tgatgacagc ggtgagcccg ccgaacgtgg cgagcgggat
     5041 cgagccctcg taggtgccct cgtccttggc gacgaccagc gtgccgagga agatgaagca
     5101 gctgatcaac gtgccgaccg cgaagaggat caggccgaga tagaagtgcg gtgccgcctg
     5161 caagggcggg taggaggtga acatcaccga ggagttgccc tgcagcaccg ccaccgtggc
     5221 gacgagggcg ccgaccagca tcaggacgta ggcgacccag gccatcatgg gtgtcgcgag
     5281 cctggagttc aggaccacgg ccgaggtgaa gtagaagagc gccacctcga agaacatgat
     5341 ccagaagatg agcacggcgg cgccgtgcgc ggtcagcacg aggtagaacc attccgccgg
     5401 cagcagatgg atggcctgcc agcgggtcag ggcgatgagc aggccgaaga gcccgccgac
     5461 ggcgaggaag gcgaccgccg tcaggacgtt gacgcggatc agcgtctccg ccttggcatg
     5521 cacctcgaga ccggtgtcgg gacaggtgcg gaaacgctcc tccaccacct ggcgcccggg
     5581 aaccgcaata ctcgccatgg ctactctccc gccgccagtt gcatgccggc ggccttggcc
     5641 gcctcccggt cgaggacgtt gatccggccg gtcatcaggt gatggccgat gccgcagaac
     5701 tcgttgcaga cgatgccgaa ctcgcccgat tcgttggggg tcagcgtcac caccatctcg
     5761 tagccggggt ggacctgcag gttgatgttg gtcggctgca gcgagaagcc gtgctgccag
     5821 tcgagcgagg agatgtgcag gcggtaggtc tcgccctcga ccagctcgag gatcggcgac
     5881 cagtcccaga gccgggcctg cagatagacg tcggcgccga ccggcgggcg gacgatcggc
     5941 atgccgaact cgtcctcgcc gacctggtat tcctcgatca tcgcctcgac ccgctcggcg
     6001 tagacctcgg gcaggatccg gtaggactcg ttggagaggt tctgctcacc atagatgtgc
     6061 caccagatca tggtggagaa catcgcgatg ccccagagaa aggcgatggt gatccagacg
     6121 atctccatgc gatgcagcgg ctcgttccac cacaggcggt tcttcggcgg caggagcgcc
     6181 atggccttct cctaggtgct caggacggag cgactggaat ctgggcgatc tccatgatcc
     6241 cccagatcag gtagagaacc gtcggcatgg tgacgccgat gaagaccagg aggaacgggt
     6301 tgtcgagcag ccgctgcatg acatgcggcc gccccccttc ctcagcttcc gtcctttgtc
     6361 gtgccacggc cgagctcccc ttatccggcc acgatcggcg gtggcggctg gccgcccgcg
     6421 atcgagggtg cgctgatgaa tcacgctagg gccgtgccgg cggcgctgtc cttgaccgcc
     6481 gtcaagtggc cgcaagggcg ctcgacgtca tgtcgacggc ccgtttgcac cccgggtgac
     6541 gtcatcgagt gaagcccggg aagaggttgg ccgcgtagcg cgcatggcag ccggcgcagg
     6601 cctcgaccac ctccccgaac agctcgcgct ggcgggcctg ctcgcccgcg cgggcggctt
     6661 cggcgaggct ggcggcgagc tcgtggaagg cacggtcgag ctcgacgaat tccgctggcg
     6721 cgacggccag caaagcctgc atgtcggcat cggtcatctg ctgctccagg atgaagctgt
     6781 cgtggatcgc ctgtgccttc tcggccacca cggcgtcctc gccgcgcacc agggcgtcca
     6841 ggatcatctg ggtggcgccg agcaccgccg ccatctcctc gagcagcatg gcgcgcacgt
     6901 tcggcggcag cagggggccg atggcgtcgt cggcatgcgc cggcgaggtc ggtgccatgg
     6961 ccgccagccc gcccacgagc gccagcgcca gcaccgcatt cgatcggcgc ttcggcgatt
     7021 gcagcgaatt gtcacgccag tgacgcggtt ccatggcctc tcccttcctt gcacggaccg
     7081 atcgcgagac gacaagcggc tagctgaaac gatcgcatcg acccggtcaa gtgcccagga
     7141 acgatcggat cagcgcgaag accggcagcg ccagcccgag gagcaggagg aagaagatca
     7201 ccgagcggtt cggcaagggc ccgccccgcc gccgctcgac gagggcgaga agccggtccg
     7261 cggcgaggta gagcgtccct gcgacgacgg tgaagagaac gatctccatg catacctccg
     7321 gttgccatgc ctgcccggat gggcttcgat ggggtgggcc acctaggggc cggacgggct
     7381 gcgcctcctt gatcgcgatc aaggatcggg gcactctcgg cgctcgacgg tcccccgacg
     7441 acagggcggg tcggcgcaca agggggatgt gatggtgcat gcgtgtcggc cacccttgga
     7501 tccgggcggc tcgccgcctt tgcgcgggct ggctgcggcc gacgccgtgc gcctcgcgcg
     7561 gctgccgatg ttcgccgatc tggcgcaggc caagcgtgcc gagatcatcg aggaggcgag
     7621 cgtcgttgcc gtctcgcgcc gcaccgtgct cttctaccag ggcgagcccg ccacgcgctt
     7681 ctacgtcgtg ctcgacggct gcgtgatgct gcaccgggtc agccccaacg gccaccagag
     7741 cgtcatcagc ctgatcaacc gcggcgaatc cttcgccgag gcggcgatgt tcgactgcgc
     7801 cgacttcccg gtcaccgcga ccgtcatcgc cgatgcccgt ctgctcgtcg tcaccgccca
     7861 gtccttccgc cgccggctca tgtccgaccc gtccctggcg ctcaacatcc tggcctcgat
     7921 gtcccgccag atgcgccggc tggtcgagca gatcgagcag cgctcgtcga gctcgtcgac
     7981 cgagcgtctg gcgcgcttcc ttctgcagct ctgcccggag gacgtggccg ccaccgagct
     8041 cgagctgccg ctcgacaagc aattgatcgc cggtgcgctc ggcatgcagc ccgagacctt
     8101 ctcgcgctgt ctctcccgcc tcaagccgct cggcgtgcgg gcgcacggca accatgtcga
     8161 gatcgccgac atcgctgcct tgcagcggct ctgctccggc gacggcaccc cacggatgcg
     8221 gcccggcgga ccgggccgcc gctgaccggc tagcggcgga ggaggaagaa gaagggccgt
     8281 ggcgaacgcc gcagctccat cagcccgagc acccggtcgg cgtcgatccg gcggaaatgg
     8341 tcggtgatcg gctgccggtc gtagaccatg gccgcactcg accggccgcg aaagacgcgc
     8401 gtctccagct tcgcggccgg cccgcgggcg cgaagcagcg ggcgaagggc cctgaacgcc
     8461 gccctgaccg ggcggctgcg actgaagccc ggcaggcgga gcgccagccc gaccggcagc
     8521 caggccggct cgagggccac gggcacgccc aaggggcccc ggaagagcag cgggtgcacg
     8581 cgatcgacgg cctcgaattc cttgccctgc cagccgagca cctcgagcag gccgtcgagc
     8641 gggtggccgg tcggcaggct gcacccccgc cagcttccga gcatggcctc gggctcgagg
     8701 cccggcagcg cgtcgaagcg gtcgagggcc gccgccgggt cgatcgcccg ggcccgccag
     8761 gccaggagcc agccgggtgc ggcggccggc acgtgcctac gaggcgagcg tgagctggac
     8821 cttcaccgcc ctgctgcggt cgccggcgag ctcgaaggcg tccctggcgt cctcgatcgg
     8881 gaaatcgccg gtcaggatcg gcgtcgggtc gatccggccc tcgtcgatca gccggacggc
     8941 cagggcgaac tcggggtgga aacggtgcgt gccaacgagc tcgatctcct tggagacgat
     9001 gaggttgagc ggcaccgcca cgtcgccggt gacgcccacc tggacgatcc ggcccatggg
     9061 ccgcgtcgtc tcgatcgcgg tgcggatggc aggtgcggcg gccgagcact cgaaggtgac
     9121 gtcgaaacac cccttgtcgg ccttgaattc gtccatcgcc ccggcctcgc gcgaaaggtt
     9181 gaccgcgcgc gtggcgccca tggccatggc ggcggcgagc gtggcgtcgt gcaggtcggt
     9241 gacgacgatc tcggcggccc cgcccgtggc cgcggcggcg acgcagaggc tgccgatggg
     9301 gccggcgccg gtgaccagca cgcgccggcc cctgaggtcg cccgcccggc tccgggcgtg
     9361 caggcagacc gccagcggct cggcgcaggc ggccttggcg aggctcgttc cggcaccgac
     9421 cggcacgcat tgcgcggcct cgacgacgag gcgctcgcgg aacaggccct gctcgtgcgg
     9481 gaagcgcatg gccgagccgc gaaagcgcat ctgcaggcaa tgctggggct tcccctcctg
     9541 gcagtagccg cactcgccgc agggccggct cgggttgacg gccaccttcg tgccgatggc
     9601 gagatcgacg ccggggccga ccgcctcgac cgtgccggcg gcctcgtgac cgaggatgat
     9661 cggctcctgc acccggatcg ggccgaagcc gccatgctgg tagtagtgca ggtcggagcc
     9721 gcagatgccg cccgccccca tgcgcaggcg cacctcgccc gggccgggtg ccgcgacatc
     9781 gatgctctcg atgcggatgt cgccttgggc atggagacgg cagacgcggg tttgcatggg
     9841 ttggcctttc ctagggcatg aggacggagg gcagccaggt cgcgatcggc ggcacgtagg
     9901 agaccagca
//
LOCUS       DAOTFI010000188         9706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8337_length_9706_cov_6.615791, whole genome shotgun sequence.
ACCESSION   DAOTFI010000188 DAOTFI010000000
VERSION     DAOTFI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8337_length_9706_cov_6.615791"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="P5558_17325"
     CDS             <1..828
                     /locus_tag="P5558_17325"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HRY26134.1"
                     /translation="FENAAAKGDPYAMNSLGRAYRDGTGVEADREQAIDWFLKAAAQG
                     HTFAYNNLGYMLAEAGEAERALPLFQASAASGDIYGYNNMGYAYQNGLGVETDIDAAI
                     GWYEKAAEGGQPNAPINLGFLYRNGAPGLEPDPRRAAFWFAEAAKGGNAWGSVHLAAL
                     YWDGLLGDGPDPVLAAKILARGARLDTVQGRIAAQQNYGGAGAEARARFADLPARAVV
                     QAVQEELARAGYDPGPVDGLPGQRTERAIAEFTAAQQPPLPADIAPIELLAELVTVG"
     gene            910..1614
                     /locus_tag="P5558_17330"
     CDS             910..1614
                     /locus_tag="P5558_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRY26135.1"
                     /translation="MSDGPIVFTRDGARRGAVAMLPLLPGVVAFGLLYGFLAIQAGLS
                     VLQVGLFSAFVFAGASQFLALELWQEPLPVITLLVGVVVLNLRHVLMGAAIMPWLRPL
                     GGWRAHASLYFMTDESWGMAMAEQQRGGRDAAFMAGAGVTLWVFWVTSSLAGTMAGDV
                     RAVVERFGLAFLTTAFFVVLLAGFWRGRADLLPWGVSAGIAVLAKALLPGTWYIVLGA
                     MAGSLVAALLKRGIHA"
     gene            1607..1903
                     /locus_tag="P5558_17335"
     CDS             1607..1903
                     /locus_tag="P5558_17335"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRY26136.1"
                     /translation="MLEIGVILLLAVATYATRIGGYRLAQRLQPSPFVEAFMTAMPGT
                     IFAALVAPMVLAAGPAGWVSAGVGFVAMRRLGNFFLALALAMAAYLTLVLTMGA"
     gene            complement(2015..2452)
                     /locus_tag="P5558_17340"
     CDS             complement(2015..2452)
                     /locus_tag="P5558_17340"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRY26137.1"
                     /translation="MRGIAIYLALLVVIRLSGRRTIGEMAAFDFVLLLIVAETTQQAL
                     LGDDFSITNSLVLIVTLFSADAFFSYVKQWWPQAAKWMDGQATVLISLGNPDRRALSR
                     ARVDLADVLLAAREQHGLERLDQIKFAVLEASGSISIIPKRDE"
     gene            complement(2475..3014)
                     /locus_tag="P5558_17345"
     CDS             complement(2475..3014)
                     /locus_tag="P5558_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26138.1"
                     /translation="MQFGVVHEMADLVIEAPRIRDDGLQLEEDRAFQERFWRGERIAL
                     GVFAIIILLALLGFAGSGGLLGLGAVASSAGSVEHARVVRWQTPDRLRVVLTLDQAVH
                     EVVLADPFSAWFDIETIQPEPERSFIGPHGLVLQFNTDGVPSSAVLLSVRPDAPGLAR
                     YRVVLDGAEPMTVTQLVLP"
     gene            3381..3959
                     /locus_tag="P5558_17350"
     CDS             3381..3959
                     /locus_tag="P5558_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26139.1"
                     /translation="MTTSRRTTLDAGAIACLAAIVMGGPAFSAEDATALIADALRAAP
                     PNIRDTATVSDLEGNILRPGPGDYTCFPAPQGVAGPMCMDGEWRRWMQAWMHHEPFTA
                     SRVGISYMMAGDSSDGGASNIDPAAGTPTADNDWVVEGPHVMMIVPDPAALEGLPTTL
                     DTDGPYVMWSGTPYAHIMIPIDNRPEQRAITN"
     gene            complement(4074..5054)
                     /locus_tag="P5558_17355"
     CDS             complement(4074..5054)
                     /locus_tag="P5558_17355"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY26140.1"
                     /translation="MLERVLLGTAIAAVTAMPVAAQDLLGSGMTIFAQMGGPPGGPAT
                     LPRANGAKDAARHLGADLVEQYSGWLPDVMIQQFREAVASSPDCIVIMGHPGVDAFQD
                     LVDEAEAAGILVTVNNTPLSSLQQQYGGNGMGYAGVILYDGGWVTAQNMLATGKLQPG
                     DKALVYGLLGQAERGESTRGLKEGLEEGGVVVDYLEISPEVNNDTSLAVPILVSYLES
                     NPDIKAFGTQHGGVTGQIARVLEQAGKAPGDIVVGGIDLAPSTIDGLEQGFITATLDQ
                     QLYLQGFLPVTQCVLSKKYGFAGLSINTGAGVVTPETIGELIPLIEAGIR"
     gene            complement(5087..6121)
                     /locus_tag="P5558_17360"
     CDS             complement(5087..6121)
                     /locus_tag="P5558_17360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26141.1"
                     /translation="MGGRLGRIEGLAIIIVAIVVTGVFMVMAPQTFLSDRIYATLLVT
                     MAPLLLCALGLTLVIASGQIDLSFSSIVALSGFVLAAAYRFENIPFLAFLAEGSDAAA
                     IEATRAWLIWVGAVLAVLAGAGAGWINGVLVARLGIPSIIATLATLFLWRGVAVVLAG
                     GLSYNLRGVRDTALWQVTTGRLLDWLPLQALWVGLVAVGLWFILNRHRFGEALLFLGD
                     NAEVARVMGIDVAATRIRLFTLMGALAGLAGVFLTAENITFYTNQGQGFLLLAMAAVF
                     IGGTSIYGGSGSITGTVLGAYIVLVVQAGIVAAGVQGFWNDVVIGLIFIAAVVFHLSL
                     ERTERAGGSS"
     gene            complement(6121..6894)
                     /locus_tag="P5558_17365"
     CDS             complement(6121..6894)
                     /locus_tag="P5558_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26142.1"
                     /translation="MVEAPAVELRGITKRYGKVEALGGVDLEVGRNEILGLIGDNGAG
                     KSTLIKILTGVERPTLGQMQIKGEPVDFGRYSVRGAHERGIETVYQQKSLGDKQPLWR
                     NFFVGRNITNKWGFIDVRRQKELANEVLKGAIGFRGVGIDVDATAGNLSGGERQGICI
                     GRAMHFDADLIILDEPTVALAVKEVGKVLSFIERIKASGRACLYVEHNLAHVHAMADR
                     MVIIDRGRIVGEVRRGEMTVHELTEHFLDLQRRGERGAA"
     gene            complement(6958..7428)
                     /locus_tag="P5558_17370"
     CDS             complement(6958..7428)
                     /locus_tag="P5558_17370"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HRY26143.1"
                     /translation="MLMSLLAACGFGAASDAERLALDPLTLERTGWPNDYLVCPAERC
                     TAPADRTAPVLDLPALDQLRLWEEMAAMSERTRILGMDETALTLHVEQRSRIFGFIDT
                     IVVHVLPFDDGLPTAERSTFAAYSRSELGFGDMGVNQARLESWIAQVEAVAGTR"
     gene            7707..8912
                     /locus_tag="P5558_17375"
     CDS             7707..8912
                     /locus_tag="P5558_17375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY26144.1"
                     /translation="MTAAGDATDGPPHGQAILLLSLAGFFSIALLRSCDPLVPEIARS
                     FDASIAEASAPVIAYAVAYGMGQLLFGPVGSRLGTFRTASIGCTFAALFSLLCAFVAS
                     LDQLVAARTLAGFAAGGIVPMSIAFIGETVPYSQRQTTLARLMTGAILGSIAGFAVSG
                     MLAHYMDWRYIFALLSLGLAAVTGGLWLHLPRLARQAAQRSGFLVNPLRQYARILRLR
                     WPRVVLVAVAIEGAVFGVVPFIGVLLAREHGLGNLGIGAVLGCLGLGSLTFAVLAGRI
                     IARLGEFGCARIGGAVALGGFALVAISPAPLLVGVGAYAAGLGYMMLHNTLQANATQM
                     VPEDRSGAVSLFAFCLFTGQASGVALFAAITPLVGFRPAIMASGVVVLAVGLAFAAAK
                     QRMVAAAEG"
     gene            complement(9047..9301)
                     /locus_tag="P5558_17380"
     CDS             complement(9047..9301)
                     /locus_tag="P5558_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRY26145.1"
                     /translation="MGILAASADERVRDVRFDDNTLSVDLMDGRTITVPLAWYPKLLD
                     ASAADRANWEVAGGGYGLHWPALDEDLSTEGLLRGAPAPA"
     gene            complement(9276..9518)
                     /locus_tag="P5558_17385"
     CDS             complement(9276..9518)
                     /locus_tag="P5558_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRY26146.1"
                     /translation="MPTIGRWRGHRFYFYSHEPNEPPHVHVDSSNRTAKFWLQPVALA
                     RNVGFRADELGALIAHVREHAAEYLEAWNGHFGGER"
     gene            complement(9525..>9706)
                     /locus_tag="P5558_17390"
     CDS             complement(9525..>9706)
                     /locus_tag="P5558_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26147.1"
                     /translation="NPEQLWETTLDPTIRSLIQVKVDHADEADDIFTTLMGDIVEPRR
                     DFIQENALKVVNLDV"
BASE COUNT         1453 a         3355 c         3384 g         1514 t
ORIGIN      
        1 ttcgagaacg cggcggccaa gggcgacccc tatgccatga acagcctcgg ccgcgcctat
       61 cgcgacggca ccggtgtcga ggccgatcgc gagcaggcga tcgactggtt cctcaaggcc
      121 gcagcgcagg gccacacctt cgcctacaac aacctcggct acatgctggc cgaggccggc
      181 gaggccgagc gcgccctccc gctcttccag gcctcggccg cgagcggcga catctacggc
      241 tacaacaaca tgggctacgc ctaccagaac ggcctcggcg tcgagaccga catcgacgcg
      301 gcgatcggct ggtacgagaa ggcggccgag ggcggccagc ccaacgcgcc gatcaacctg
      361 ggcttcctct accgcaacgg cgcccccggt ctcgagcccg atccgcgacg tgcggccttc
      421 tggttcgccg aggcggccaa gggcggcaat gcctggggct ccgtgcacct ggcggccctc
      481 tattgggacg gcctcctcgg cgacggcccc gatccggtgc tcgcggccaa gatcctcgca
      541 cgcggggcgc gcctggacac cgtgcagggg cggatcgcgg cgcagcagaa ctatggcggc
      601 gccggtgccg aggcgcgggc ccgcttcgcc gatctgccgg cccgcgccgt cgtccaggcg
      661 gtgcaggagg agctcgcccg cgccggctac gacccggggc cggtcgacgg cctgccgggc
      721 cagcgcaccg aacgggcgat cgccgagttc acggcagcgc agcagccgcc gctgccggcc
      781 gatatcgccc cgatcgagct cctcgcggag ctggtcaccg tgggttgagc gaaggcgacg
      841 gaagggccgg gggaaatgat ttcccccgca gccccctcgg ttttctgcgc gtggccgggt
      901 gtcgtggttg tgagtgacgg accgatcgtc ttcacccggg acggggcgcg gcggggggcg
      961 gtggcgatgc tgccgctgct gccgggggtc gtcgccttcg gcttgctcta cggctttctc
     1021 gcgatccagg cggggctctc ggtgctccag gtcggcctct tctccgcctt cgtcttcgcc
     1081 ggcgcctcgc agttcctggc gctcgagctc tggcaggagc ccctgccggt gatcacactc
     1141 ctggtcggcg tcgtcgtcct caacctgcgc cacgtgctga tgggggcggc gatcatgccg
     1201 tggctgcggc cgctcggcgg ctggcgggcg catgccagcc tctacttcat gaccgacgag
     1261 agctggggca tggccatggc cgagcagcag cggggcgggc gcgacgccgc cttcatggcc
     1321 ggcgccgggg tgacactctg ggtcttctgg gtgacgagct cgctcgccgg caccatggcg
     1381 ggcgacgtca gggcggtggt cgagcgcttc ggcctcgcct tcctcaccac cgccttcttc
     1441 gtcgtcctgc tcgccggctt ctggcgcggc cgcgccgacc tcctgccctg gggcgtctcg
     1501 gccggcatcg ccgtgctcgc caaggcgctc ctgcccggca cctggtacat cgtcctcggc
     1561 gccatggccg gcagcctcgt ggcagccctg ctgaagcgag gcatccatgc ttgagatcgg
     1621 cgtcatcctg ctcctggccg ttgccaccta cgcgacccgc atcggcggct accgcctcgc
     1681 ccagcgcctc cagccgagcc ccttcgtcga ggccttcatg accgccatgc cgggcaccat
     1741 cttcgccgcc ctcgtggccc ccatggtgct cgccgccggc ccggccggct gggtctcggc
     1801 cggcgtcggc ttcgtcgcca tgcgccggct cggcaatttc tttctggcgc tggccttggc
     1861 catggcggcc tatctcacgc tggtcttgac gatgggggcg tgaagctccg ggggaaggca
     1921 caccaaccct gctcacggac gatttgatgg cggcgatggg cgccggcggc actgctgact
     1981 ggccggccgt ggtgactcgc cgtgcgaggc cggatcattc gtcgcgtttc gggatgatgc
     2041 tgatgctgcc gctggcttcc agcacggcga acttgatctg atccagccgc tccagcccgt
     2101 gctgctcgcg tgcggccagc aagacgtcgg ccagatccac ccgcgcccgc gacaaggcgc
     2161 gcctgtcggg attgccgagg ctgatcagga ccgtcgcctg tccgtccatc catttggcag
     2221 cctgcggcca ccactgcttc acataggaga agaacgcgtc cgctgagaag agcgtgacga
     2281 tgagcaccag ggaattggtt atcgagaagt cgtcgccgag cagcgcctgc tgcgtcgtct
     2341 cggccacgat cagcagcagc acgaagtcga aggctgccat ctcgccgatc gttcgacggc
     2401 ccgacaggcg gatgaccacg agcagcgcca gatagatcgc gatgccgcgc aagaccgttt
     2461 ccatctgggc tacctcaggg caggaccagt tgagtcacag tcatcggctc cgctccgtcc
     2521 agcacgacgc ggtagcgcgc cagtcccggc gcatccggcc gcacgctcag caggactgct
     2581 gaactcggca ccccgtccgt gttgaactgc agcaccagcc cgtggggacc gatgaagctg
     2641 cgctcgggtt cgggctggat ggtttcgatg tcgaaccagg cggagaacgg gtcggccagc
     2701 accacctcgt gcaccgcctg atccagagtc aggacgacgc gcaatcggtc gggagtctgc
     2761 cagcggacga cacgcgcatg ctcgacgctg ccggcgctgc tcgccacggc acccaacccc
     2821 aggagcccgc cggaaccggc gaagcccaag agcgccagga ggatgatgat ggcgaagacg
     2881 ccgagcgcaa tgcgctcgcc ccgccagaac cgctcctgaa aggcccggtc ttcctccagt
     2941 tgaagaccgt cgtcccgaat ccggggcgcc tcgattacca ggtcggccat ttcatgcacc
     3001 accccgaatt gcatgccgag cattccccgg cagcgacaaa cgcggcggct ggactgctgg
     3061 ttcccgccgc atctgcgagc gaacccggcc tcctgtccgc gacgacggcg agccggtcgt
     3121 cggcgagccg cgccgcatcg ccgccatgcc gccgccgccg gaccagcccc tccagcggtg
     3181 aaggtgaccc tcgccccgcg tgcacgcggc ggatgagctg aacgcggagc gcgtgcggcg
     3241 caggtcaaaa agggggggcc tcgatagccc gcacgccggg ctacgtcatc ggtggcgctg
     3301 cccctacgcc agttgaccga tgccgttctt gatcggtgtg tgcgaacgtc gaatcctcat
     3361 cgccaatgga ggaccgaacg atgaccacct cgagacgcac gaccctcgac gccggcgcga
     3421 tcgcgtgtct tgctgccatc gtcatgggtg ggcccgcgtt cagcgccgag gatgcgacgg
     3481 cactcatcgc cgacgcgctc agggcggcac cacccaacat ccgagacacg gccactgtct
     3541 ccgatctcga aggcaacatc cttcgtccgg ggcccggcga ctacacctgc tttccggcac
     3601 cgcagggggt cgccgggccg atgtgcatgg atggcgaatg gcgacgctgg atgcaggcct
     3661 ggatgcatca cgagcccttc accgcgtcac gggtcggcat ctcctacatg atggccggcg
     3721 actcgtcgga cggcggcgcc agcaacatcg acccggcggc gggaacgccc acggccgaca
     3781 acgactgggt ggtcgaaggg ccgcacgtca tgatgatcgt gcccgatccg gcggcgctgg
     3841 agggactgcc taccaccctc gacacggacg gcccctacgt catgtggtcg ggcacgccct
     3901 acgcccacat catgattccg atcgacaacc ggccggagca gcgggcgatc accaactagc
     3961 gccgcagggc gggcttgccg tgccgggtga tggtcacctc ctcgccctct tgcgcaaggt
     4021 cgagacgtgc cgaggggaac cgggggaact cagttccccc ggttccttgc tctctaccga
     4081 atcccggctt cgatcagcgg gatcagctcg ccgatggtct ccggcgtcac cacgccggcg
     4141 ccggtgttga tggagaggcc ggcgaagccg tacttcttgc tcaggacgca ctgcgtcacc
     4201 ggcaggaagc cctggaggta gagctgctgg tcgagcgtgg cggtgatgaa gccctgctcg
     4261 aggccgtcga tggtcgaggg ggcgaggtcg atgccgccga cgacgatgtc gccgggcgcc
     4321 ttgccggcct gctcgaggac gcgggctatc tggccggtga cgccgccgtg ctgggtgccg
     4381 aacgccttga tgtcgggatt gctctcgaga tacgagacga ggatcggcac ggcgagcgag
     4441 gtgtcgttgt tcacctcggg gctgatctcg agatagtcga cgacgacgcc gccttcctcg
     4501 aggccctcct tgaggccgcg ggtggattcg ccgcgctcgg cctggccgag gaggccgtag
     4561 accagggcct tgtcgccggg ctgcagcttg ccggtggcca gcatgttctg ggccgtgacc
     4621 cagccgccgt catagaggat gacgccggca tagcccatgc cgttgccgcc gtattgctgc
     4681 tgcaggctgg agagcggcgt gttgttgacg gtgaccagga tgccggcggc ctcggcctcg
     4741 tcgaccaggt cctggaaggc gtcgacgccc gggtggccca tgatgacgat gcagtcgggc
     4801 gacgaggcga cggcctcgcg gaactgctgg atcatgacgt cggggagcca gcccgagtac
     4861 tgctcgacga ggtcagcgcc gagatggcgg gcggcgtcct tcgccccatt ggcgcggggc
     4921 agggtggcgg ggccgccggg ggggccgccc atctgggcaa agatggtcat gccggagccc
     4981 aggagatcct gggctgccac cggcatggcg gtgaccgcgg cgatggcggt gccgagcagc
     5041 acgcgttcga gcatcgactt gtccttccat tctgagaccg agtggttcaa ctgctcccgc
     5101 cggcacgctc ggtccgctcc agcgagaggt ggaagacgac ggcggcgatg aagatgaggc
     5161 cgatcacgac gtcgttccag aagccctgca cgccggcggc gacgatgccg gcctgcacga
     5221 cgagcacgat ataggcgccg aggaccgtgc cggtgatgct gccggagccg ccatagatcg
     5281 aggtgccgcc gatgaagacg gcggccatgg cgagcagcag gaagccctgg ccctggttgg
     5341 tgtagaaggt gatgttctcg gcggtgagga agacgccggc gagcccggcg agcgctccca
     5401 tgagcgtgaa gaggcggatg cgggtggcgg cgacgtcgat gcccatgacc cgcgccacct
     5461 cggcattgtc gccgaggaag agcagcgcct cgccgaagcg gtggcggttg aggatgaacc
     5521 acaagccgac ggcgaccagc ccgacccaga gcgcctggag cggcagccag tcgaggaggc
     5581 ggccggtggt gacctgccag agggccgtgt cgcgcacgcc ccggaggttg tagctgagcc
     5641 cgccggcgag gacgacggcc acaccgcgcc agaggaagag ggtggccagg gtggcgatga
     5701 tcgaggggat gccgagccgg gcgacgagca cgccgttgat ccacccggcc ccggccccgg
     5761 cgaggacggc gagcacggcg ccgacccaga tcagccaggc gcgggtcgcc tcgatcgccg
     5821 ccgcatccga gccctcggcg agaaaggcca ggaacggaat gttctcgaag cggtaggcgg
     5881 cggcgaggac gaagcccgag agcgccacga tggaggagaa ggagaggtcg atctggccag
     5941 acgcgatgac caaagtgagg ccgagcgcgc agagcagcag cggcgccatg gtgaccagga
     6001 gcgtcgcgta gatgcggtcg gagaggaagg tctgcggcgc catgaccatg aagacgccgg
     6061 tgacgacgat cgccacgatg atgatcgcca gcccctcgat ccggccgagc ctgcccccca
     6121 tcaggcggca ccccgctcgc cccggcgttg caggtcgagg aagtgctcgg tgagctcgtg
     6181 cacggtcatc tcgccccgcc gcacctcgcc gacgatgcgg ccgcggtcga tgatcaccat
     6241 gcggtcggcc atggcgtgga cgtgggcgag gttgtgctcg acatagaggc aggcgcggcc
     6301 cgaggccttg atccgctcga tgaaggagag gaccttgccg acctccttga cggcaagcgc
     6361 cacggtgggc tcgtcgagga tgatgaggtc ggcgtcgaaa tgcatggccc ggccgatgca
     6421 gatgccctgc cgctcgccgc ccgagagatt gccggccgtg gcatccacgt cgatgccgac
     6481 cccgcgaaag ccgatggccc ccttgagcac ctcgttggcc agctccttct gccgcctgac
     6541 gtcgatgaag ccccatttgt tggtgatgtt gcggccgacg aagaaattgc gccagagcgg
     6601 ctgcttgtcg cccagactct tctgctggta gacggtctcg atgccgcgct catgggcgcc
     6661 gcgcacgctg tagcggccga agtcgaccgg ctcgcccttg atctgcatct ggccgagggt
     6721 gggccgctcg accccggtca ggatcttgat gagcgtcgac ttgccggccc cgttgtcgcc
     6781 gatcaggccg aggatctcgt tcctgcccac ctcgaggtcg acaccaccca gcgcctcgac
     6841 cttgccgtag cgcttggtga tgcccctgag ctcgaccgct ggcgcctcga ccaattcttg
     6901 cctccccgct cttcctgccc gcgagcttgg gcgctgggcc cggggcggtc aagccggtca
     6961 gcgcgtgccg gcgaccgcct cgacctgggc gatccagctc tccagccggg cctggttgac
     7021 ccccatgtcg ccgaagccga gctcggacct ggaataggcg gcgaaggtgc tgcgctcggc
     7081 cgtgggcagg ccgtcgtcga agggcaggac atggacgaca atcgtgtcga tgaagccgaa
     7141 gatgcggctc ctctgctcga catggagggt gagggcggtc tcgtccatgc cgaggatgcg
     7201 ggtccgctcg ctcatcgccg ccatctcctc ccagagacgg agctggtcca gcgccggcag
     7261 gtcgagcacc ggcgcggtgc ggtcggccgg tgccgtgcag cgctcggccg ggcagacgag
     7321 atagtcgttc ggccagccgg tgcgctcgag cgtcaggggg tcgagggcga ggcgctcggc
     7381 atcggaggcg gcaccgaagc cgcaggcagc gagcaggctc atcagcatca gcagtgccgc
     7441 cgttccggcc ggccaccgtg tgtcactcgc gccgcgcctg gcgatccgct ccggcatggg
     7501 ccacatcagc gcgagcgttc gtcgctggcg cacatcgcga cgggaccggt ctcgtggagg
     7561 tcggcagcga tcatgctcgc aaaatacgcc ccgatggcgg ccgcggcaag ccggcggggc
     7621 ggcagtccgg gcgggggcgg ccccgctctg gcaccccagg cggaacgacg ctaggctccg
     7681 cccgtctcga aacgggcggg ccacacatga cggcggcagg tgacgcgacc gacggcccgc
     7741 cgcacgggca ggcgatcctc ctgctcagcc tcgccggctt cttctcgata gcgctcctgc
     7801 gctcctgcga tccgctggtg ccggaaatcg cgcgcagctt cgacgccagc atcgccgagg
     7861 cctcggcacc ggtgatcgcc tatgccgtgg cctacggcat gggccagctt ctcttcgggc
     7921 cggtcggcag ccggctcggc accttcagga ctgccagcat cggctgcacg ttcgcggccc
     7981 tcttctcgct gctctgcgcc ttcgtggcca gcctcgacca gctcgtcgcc gcccgaaccc
     8041 tcgccggctt cgccgcgggc ggcatcgtgc cgatgtcgat cgccttcatc ggcgagacgg
     8101 tgccctacag ccaacggcag acgacgctcg cgcgcctgat gaccggcgcc atcctgggct
     8161 cgatcgccgg cttcgcggtc tcgggcatgc tcgcccacta catggactgg cgctacatct
     8221 tcgcgctcct gtccctcggt ctcgccgccg tgaccggcgg cctctggctg catctgccgc
     8281 ggctcgcgag acaggcggcg cagcgcagcg gctttctggt caacccgctg cgccagtacg
     8341 cgcgcatcct ccgcctgcgc tggccgcgcg tggtgctggt cgccgtcgcc atcgaaggcg
     8401 cggtcttcgg ggtcgtgccg ttcatcgggg tcctgctggc cagggagcac ggcctcggca
     8461 atctcggcat cggcgccgtg ctcggctgcc tcggcctcgg cagcctgacc ttcgccgttc
     8521 tcgccggccg gatcatcgcc cggctcggcg aattcggctg tgcccgcatc ggcggtgccg
     8581 tggcgctggg cggcttcgcc ctcgtcgcca tctcccccgc gccactactg gtcggcgtcg
     8641 gcgcctacgc cgccgggctc ggctacatga tgctgcacaa caccctgcag gccaacgcca
     8701 cccagatggt gcccgaggac cgcagcggcg ccgtctcgct cttcgccttc tgcctgttca
     8761 ccggccaggc ctcgggcgtc gccctcttcg ccgccatcac cccgctcgtc ggcttccggc
     8821 cggccatcat ggccagcggc gtcgtcgtgc tggccgttgg cctcgccttc gcggcggcga
     8881 agcagcgcat ggtggcggcg gccgagggct gaacgtgccc ggtatgaaag aggaaggagc
     8941 agggaaggcg tgcctaccct ggccccaccg gtctttgccg gtgaaggccg tggcggacga
     9001 agcgcaggtg acgaggggct gggcggtggg gacggctgcg gctgtttcag gcgggtgcgg
     9061 gagcgccacg gagcaggcct tcggtgctga ggtcctcgtc cagcgcgggc cagtgcaggc
     9121 catagccgcc accggccacc tcccagttgg cccggtcggc agcgctcgcg tccagaagtt
     9181 tcgggtacca ggcgaggggg acggtgatgg tccggccgtc catcaggtcg acgctgagcg
     9241 tgttatcgtc gaaacggacg tctcggacgc gttcgtcagc gctcgccgcc aaaatgccca
     9301 ttccaggcct ccagatactc ggccgcatgt tcacggacat gagcaatcaa agcgcccaac
     9361 tcatccgcgc gaaacccgac attacgcgca agcgcaaccg gctgcagcca gaatttcgcg
     9421 gttcggttgc tcgaatcgac gtgaacgtgc ggcggctcat tgggctcatg gctgtagaaa
     9481 tagaagcgat ggccgcgcca gcggccaatg gtcggcattg acgatcaaac gtccagattg
     9541 accaccttca gcgcgttctc ctggatgaag tcgcggcgcg gctcgacgat gtcgcccatg
     9601 agcgtggtga agatgtcgtc ggcctcgtcg gcgtggtcga ccttgacctg aatgagggag
     9661 cggatggtgg gatcgagggt ggtttcccag agctgctcgg ggttca
//
LOCUS       DAOTFI010000189         9587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8475_length_9587_cov_6.501574, whole genome shotgun sequence.
ACCESSION   DAOTFI010000189 DAOTFI010000000
VERSION     DAOTFI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8475_length_9587_cov_6.501574"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="P5558_17395"
     CDS             complement(<1..245)
                     /locus_tag="P5558_17395"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY26148.1"
                     /translation="MTAPKGRLEAAGLELSRLGLGGTPFGGLHEANTDAAVAATVTAA
                     LDQGVRYLDTAPFYGFGLAEHRLGRALQDVPRERYVLS"
     gene            complement(242..1156)
                     /locus_tag="P5558_17400"
     CDS             complement(242..1156)
                     /locus_tag="P5558_17400"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26149.1"
                     /translation="MTATTVGFIGLGFMGRPMALNIMKKGHRLVVHDVDQGAVDALVA
                     RGATAAGSPREVAAAADIVITMLPDGPDVEKVALGPDGIAEGLGQDALHVDMSTIAPS
                     TTRRIGEAYAKRGLHLIDCPVGKTQDHAVAGTLTLMAGGDAAQVERARPVLMCMGDTL
                     FHCGDLGAGETIKLVNNRLAATIIGAVSEALVTGTKAGLSLDLMREVMGTTMAANAHL
                     SINMPKKSLLGDFTPGFMVALAQKDVRLAQALAAELGVDSPVGAVTLDLLGRASEAGM
                     AREDVGALLKLFEQQAGVEVRLPAAGAS"
     gene            complement(1238..2473)
                     /locus_tag="P5558_17405"
     CDS             complement(1238..2473)
                     /locus_tag="P5558_17405"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26150.1"
                     /translation="MTRETRRGAIGLTGALTIMALTLAAAPLPAFAQDLQELEVSVGR
                     QPWAAGNSPITQYMIQNGLFEAKAKELGYDLTVDYRDYPSAMPQVEAFVGGNLDFGMW
                     GNTPIVRGIAQKQPWTVLNVGEGHLRFIVVTHPDSGIRNIQDLKGKTVGALLGGDPYN
                     AFSQILHGELGSGDPAAHGISIVNTPTQAQAATVPAGMDAAIVISPFYLKAQDELGIV
                     GIVNSFGYTEPGYSGPAGEGAGHLLPSAEQSQFWPDGYYLHRSFWVVQNSVLDAHPDV
                     VTAFIAAQQQAVEELSAMPPEEVSQISADYWNLPPEMGAEVVEDEVLFIRGWVWPTEG
                     DAGALLAISQFMVEGGLIEEPLTWDQVKEAMRPAAGLMQKAWEMTGKVPEAAAFTAAD
                     TQDLRGLPVWEMDKWQARD"
     gene            complement(2519..3376)
                     /locus_tag="P5558_17410"
     CDS             complement(2519..3376)
                     /locus_tag="P5558_17410"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26151.1"
                     /translation="MSRPAPTGPKLRLDGVRKTFTVPRTGTNVVAVETADLAIEAGEF
                     LTIVGPSGCGKSTILNMLAGLELPTEGTVAMDGRTITGPAAERGMVFQDYALFPWKTV
                     FENVEFGLRYGPHGKGLSTAERRRRVARCVELVGLGGSEQKYPHELSGGMRQRCGIAR
                     LVANEPDVLLMDEPFAALDAQTRIILQEELLRIWGETGNRAERRTVVFITHAIDEAVF
                     LSDRVVVMSAHPGRIIEILDVDLPRPRTDATRTRPEFQALSQAIWNLIRDEVYRAATA
                     AGSAGGARG"
     gene            complement(3373..4152)
                     /locus_tag="P5558_17415"
     CDS             complement(3373..4152)
                     /locus_tag="P5558_17415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26152.1"
                     /translation="MSTRRWPRYFILPLGLLVLWQLWGMTLPARSRAPVPLDVLASAV
                     DLIASGRLPMALLQSLGRVGLGFLVAGSIALVLGLLMGFFRPVERNLDPIIESFRPVA
                     PIALLPLAILWLGTGTPAAVFIVAYAAFFPMIVNTIAGVKGVERRLVQAAQTLGVPQL
                     TILRTVVLPAALPSIFIGVRLAMGLAWMSIIAAELAVGSKGGGGGSGGVGEMMFVFYA
                     YYIELDSIVVCMIAVGLVALAIDHGLRWLQRWLMPWAEQHR"
     gene            complement(4149..4955)
                     /locus_tag="P5558_17420"
     CDS             complement(4149..4955)
                     /locus_tag="P5558_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26153.1"
                     /translation="MARAADSGAPPGRQWRQSTVLLYLRSLLALAIIWSLIALWVGNH
                     VILPQPTSVAYELFRLIASGEMLVHAAVSLWRLALSYALAALIGIPLGLLMGLWPWAS
                     DLLDPVVEILRPISGIAWIPLALFIFGIGNELPIFIITYVALFPFLLNTMAGVRATDR
                     TLVRAARTMGVDQRTIVRHVMIPSALPQILTGARIAGGAAWMSLVAAELIGAPSGLGF
                     AIEWYRQLLMTPKVIAVIVVISLLGYLTDRGLRWLQQRLTPWSTGIGANP"
     gene            5069..6085
                     /locus_tag="P5558_17425"
     CDS             5069..6085
                     /locus_tag="P5558_17425"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY26154.1"
                     /translation="MGGNAGQGQVRMVDVARRAGVATVTVSRVLHEAEKVAPATRSRV
                     EAAIAELGYLPNLVARSLVAARTGMVAALIPTIDNSLHAEMIQGMDERCAAAGLHLLI
                     GCTAFSLEVEERMLRAFLSRQPEAVYLTGSNHSPATRTMLKASRVPVVEVASLPETPI
                     DMVAGYSSYCAAAAVLRHLIERGYRRIGYVYSPIGVNDRLAERHRAYLETLASAGLET
                     PEAWRFESELGMAGGGLALARLRAQAPELDAVFCSTDVLAVGVLLECLRQGIAVPGEL
                     AVAGFDDLPIAAEIVPALTTVRIPRRRMGEVAAGLILRRLAGEAVAEPVVDLGFELQV
                     RASA"
     gene            6177..6899
                     /locus_tag="P5558_17430"
     CDS             6177..6899
                     /locus_tag="P5558_17430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26155.1"
                     /translation="MTRTVLITGAAGRIGRKVAAHLEARGGYALRRLDVRADAPDVVA
                     ADLADWDEGWAGAFSGVDTVLHLAAHPSPEIDWATAQRLNLDLTYNVLHAAGKHRVRR
                     VVFASSNWVMAGYRDQTGPLTPDMAPLPVNPYGVSKLVGERAGRSLADRGISFIGLRI
                     GYSVSGEGRPASELKMGLWGRQMWLSDRDLCQGFERSILAEGVGFTVLNLMSDNPGMR
                     WSLDETRRVLGYEPQDGYPAEG"
     gene            complement(6881..7915)
                     /locus_tag="P5558_17435"
     CDS             complement(6881..7915)
                     /locus_tag="P5558_17435"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY26156.1"
                     /translation="MSDTPVFAAIGLDHRHIHHMVGELIAAGARCAGYCPKTTDPRVL
                     EGFRERFPDLPAVDREKLLDDPGVGIICSAAVPRDRAALAIRAMRAGKDVMVDKPGCT
                     TLADLDRVEATARETGRIFSICFSERFIVPACELAARLVAEGAIGRVIQTTGLGPHRL
                     NRTIRPAWFFEHAAHGGILADIASHQIDQFLFFTGSDEVEIAASAVGSFGDHGIQGFE
                     DYGEILLRSEKASGFIRVDWFTPDGLATWGDGRLFLLGTEGYLELRKYIDIAGNAGTD
                     HVLLVNGEGTRRLDASGEPLTYFRRFLADVRDRTATAMPEGHAVRVTRLALEAQARAT
                     RLGVPQPSAG"
     gene            complement(8017..9075)
                     /locus_tag="P5558_17440"
     CDS             complement(8017..9075)
                     /locus_tag="P5558_17440"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY26157.1"
                     /translation="MMKTAILVIGILLVLASLAPLVRSDQWWIRFFDFPRLQLAIALA
                     GLIVAALILFSPASLSGAAFLLALAATLAYQAWRVFPYTPLATPEVLLSRDCEPASRV
                     RLMIANVLMDNRRAEDFLALVRGADPDLIFTVETDDWWDEQLAVLRESYPFGLARPLD
                     NTYGLHLFSRLDLDSPEIRFLVEDDVPSVRTRVRLRSGQWIDFIGLHPRPPRPGQDTE
                     QRDAEILIVGKEMKESNRPTIVAGDLNDVAWSHTTRLFRRTSGALDPRIGRGRFSTFH
                     ARYPVLRWPLDHVFHTDTFTLIEMRRLGYYGSDHFPVLIELCLEREAELVQEAPAPEG
                     NDREEARDKIEEGKEAGG"
     gene            complement(9155..>9587)
                     /locus_tag="P5558_17445"
     CDS             complement(9155..>9587)
                     /locus_tag="P5558_17445"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY26158.1"
                     /translation="AAAPFAWVLAAEQVPQRLAAAMGSLDAGLVVLLLLINLFLLFVG
                     LFIEMIAALVILVPILVPMVKAAGYDPIQFGIVLVLNLVIGALTPPLGMLVFITARVG
                     KADVTAVFRAVVPFILALLVVLMLVTFVPAFTLGPLWLFGP"
BASE COUNT         1489 a         3065 c         3671 g         1362 t
ORIGIN      
        1 gagaggacgt agcgctcgcg cggcacgtcc tgcagggccc ggccgaggcg gtgctcggcg
       61 aggccgaagc cgtagaaggg tgcggtgtcg agatagcgca caccctggtc gagggcggcc
      121 gtgactgtcg cggccacggc ggcgtcggtg ttcgcctcgt gcagcccgcc gaagggcgtg
      181 ccgccgaggc cgaggcggct gagctcgagc ccggccgcct cgagacgccc cttcggcgcc
      241 gtcacgacgc accggcggcc ggcagccgca cctcgacgcc ggcctgttgc tcgaagagct
      301 tgaggagcgc gccgacatcc tcgcgcgcca tgccggcctc gctcgcccgg ccgagcaggt
      361 cgagcgtcac ggcgccgacc ggcgaatcga cgccgagctc ggccgccagc gcctgggcga
      421 ggcggacgtc cttctgggcc agggccacca tgaagcccgg ggtgaagtcg ccgagcagcg
      481 acttcttcgg catgttgatg gagagatggg cgttggcggc catggtggtg cccatcacct
      541 cgcgcatcag gtcgagggag agcccggcct tggtgccggt gaccagcgcc tcggacacgg
      601 cgccgatgat ggtggcggcg aggcggttgt tgacgagctt gatggtctcg ccggcgccga
      661 gatcgccgca gtggaagagg gtgtcgccca tgcacatgag gacggggcgg gcgcgctcga
      721 cctgggccgc gtcgccgccg gccatcaggg tcagcgtgcc ggcgacggcg tggtcctggg
      781 tcttgccgac ggggcagtcg atcaggtgca ggccgcgttt cgcgtaggcc tcgccgatcc
      841 gccgcgtggt ggagggggcg atggtgctca tgtcgacgtg gagggcatcc tggccgagcc
      901 cctcggcgat gccgtccggc ccgagcgcca ccttctccac gtcaggcccg tcgggcagca
      961 tggtgatgac gatatcagcc gctgccgcca cctcacgggg ggagccggcg gcggtggcgc
     1021 ccctggccac cagagcatcg acggcgccct ggtcgacgtc gtgcacgacc agccggtggc
     1081 ccttcttcat gatgttcagc gccatcggcc ggcccatgaa gccgaggccg atgaagccca
     1141 cggtggtcgc ggtcatttct tcttccttgt gcgagcgggg aggtgtggcg cggagacggg
     1201 ccccgcgcca ccgtcagtcg atcagtgtcg cggacgctca gtcacgcgcc tgccacttgt
     1261 ccatctccca gaccggcagg ccgcgcaggt cctgcgtgtc ggctgccgtg aaggcggccg
     1321 cttccggcac cttgccggtc atctcccagg ccttctgcat caggccggcg gccgggcgca
     1381 tggcctcctt gacctggtcc caggtgagcg gctcctcgat cagcccgccc tcgaccatga
     1441 actgcgagat ggcgagcagg gcaccggcat cgccttcggt cggccagacc cagccccgga
     1501 tgaagagcac ctcgtcctcg accacctcgg cccccatctc gggcggcagg ttccagtagt
     1561 cggccgagat ctgggagacc tcctcgggcg gcatcgccga gagctcctcg accgcctgct
     1621 gctgggcggc gatgaaggcg gtcacgacgt cgggatgggc gtcgagcacc gaattctgca
     1681 cgacccagaa ggagcggtgc aggtagtagc cgtccggcca gaactgcgac tgctcggcgg
     1741 agggcaggag atggccggcg ccctcgccgg ccgggccgct gtagccgggc tcggtgtagc
     1801 cgaaggagtt gacgatgccg acgatgccga gctcgtcctg cgccttgagg tagaagggcg
     1861 agatgacgat ggcggcatcc atgccggccg gcacggtggc cgcctgggcc tgggtcgggg
     1921 tgttgacgat gctgatgccg tgggccgccg gatcgcccga gccgagctcg ccatggagga
     1981 tctgcgagaa ggcgttgtag ggatcgccgc cgagcagggc gccgaccgtc ttgcccttca
     2041 ggtcctggat gttcctgatg ccgctgtcgg ggtgggtgac gacgatgaag cggagatggc
     2101 cctcgccgac attgagcacg gtccagggct gcttctgggc gatgccgcgg acgatcgggg
     2161 tgttgcccca catgccgaag tcgagattgc cgccgacgaa ggcctcgacc tgcggcatgg
     2221 ccgagggata gtcgcggtag tcgacggtga ggtcgtagcc gagctccttc gccttcgcct
     2281 cgaacaggcc gttctggatc atgtactggg tgatcggcga gttgccggcg gcccagggct
     2341 ggcggccgac cgagacctcg agctcctgga ggtcctgggc gaaggcgggc aggggcgctg
     2401 ccgccagtgt cagggccatg atggtcaggg ctccggtgag cccgatggcc ccgcgtcttg
     2461 tctccctcgt catcgtcgct tctccttttg gctcggttgc cgatgagctg gtcgtccgtc
     2521 agccgcgcgc cccgcctgcc gacccggcag cggtggcggc gcgatagacc tcgtcgcgaa
     2581 tcaggttcca gatggcctgg gagagcgcct ggaactcggg ccgcgtgcgc gtcgcgtcgg
     2641 tgcgcgggcg gggcaggtcg acgtccagga tctcgatgat gcggccggga tgagcgctca
     2701 tcacgaccac ccggtcggag aggaagacgg cctcgtcgat ggcgtgggtg atgaagacga
     2761 cggtgcggcg ctcggcgcgg ttgccggtct cgccccagat gcgcaggagc tcctcctgga
     2821 ggatgatccg ggtctgcgcg tcgagggcgg cgaagggctc gtccatcaag agcacgtcgg
     2881 gctcgttggc gacgagccgg gcgatgccgc agcgctggcg catgccgccg gagagctcgt
     2941 gcgggtattt ctgctcggag ccgccgaggc cgacgagctc gacgcagcgc gccacccggc
     3001 ggcgccgctc ggcggtggaa agacccttgc cgtgcggccc gtagcgcaag ccgaactcga
     3061 cattctcgaa gacggtcttc caggggaaga gggcgtagtc ctgaaagacc atgccgcgct
     3121 cggccgccgg gccggtgatc gtccggccgt ccatggcgac ggtgccctcg gtcggcagct
     3181 cgagcccggc cagcatgttg aggatggtgc tcttgccgca gcccgacggg ccgacgatgg
     3241 tcaaaaactc gcccgcctcg atggcgaggt cggcggtctc gacggcgacc acattcgtgc
     3301 cggtgcgcgg cacggtgaag gtcttgcgca cgccgtcgag gcgcagcttg gggccggtcg
     3361 gtgccggtcg gctcatcggt gctgctccgc ccagggcatc agccagcgct gcagccatct
     3421 gaggccgtga tcgatggcga gggcgacgag gcccacggcg atcatgcaga cgacgatgct
     3481 gtcgagctcg atatagtagg catagaagac gaacatcatc tcgccgaccc cgccgctgcc
     3541 gccgccgccg cccttggagc cgacggcgag ctcggccgcg atgatcgaca tccaggcgag
     3601 gcccatggcg agccgcacgc cgatgaagat ggagggcagg gccgccggca ggacgacggt
     3661 gcgcaggatg gtgagctgcg gcacgccgag cgtctgggcc gcctggacga ggcgacgctc
     3721 gacccccttc accccggcga tggtgttgac gatcatcggg aagaaggcgg cgtaggcgac
     3781 gatgaagacc gccgccggcg tgccggtgcc gagccagagg atggcgagcg gcaaaagggc
     3841 gatcggggcc accgggcgaa agctctcgat gatcgggtcg agattgcgct cgaccgggcg
     3901 aaagaagccc atgagcaggc cgaggacgag ggcgatgctg ccggcgacga gaaagccgag
     3961 gccgacccgg ccgaggctct gcaggagggc catgggcagg cggccgctgg cgatcagatc
     4021 gactgccgaa gccagcacgt cgagcggaac gggggcgcgg ctgcgcgccg gcagggtcat
     4081 gccccagagc tgccagagga ccagcaggcc gagcggcagg atgaaatagc ggggccagcg
     4141 ccgagtgctc acgggttcgc cccgatgccg gtgctccagg gggtgagccg ctgctgcagc
     4201 cagcgcagcc cccggtcggt gaggtagccg agcaggctga tgacgacgat cacggcgatc
     4261 accttgggcg tcatcaggag ctggcggtac cactcgatgg cgaagccgag gccgctgggg
     4321 gcgccgatca gctcggccgc gacgagggac atccaggcgg cgccgccggc gattcgcgca
     4381 ccggtcagga tctgcggcaa ggccgagggg atcatgacgt ggcggacgat ggtgcgctgg
     4441 tcgacgccca tggtgcgggc ggcgcggacc agcgtgcggt cggtggcgcg caccccggcc
     4501 atggtgttca agaggaaggg gaagagggcg acataggtga tgatgaagat cggcagctcg
     4561 ttgccgatgc cgaagatgaa gagggcgagc gggatccagg cgatgccgga gatcggccgg
     4621 aggatctcga ccaccgggtc gaggaggtcg ctggcccagg gccagaggcc catgagcagg
     4681 ccgagcggga tgccgatgag ggcggcgagc gcgtagctga gcgccaggcg ccagaggctg
     4741 acggcggcgt ggacgagcat ctcgccgctg gcgatcaacc ggaagagctc gtaggcgacg
     4801 ctggtcggct gcggcaggat gacgtgattg ccgacccaaa gcgcgatcag cgaccagatg
     4861 atcgccagcg cgagcagcga ccgcagatag aggagcacgg tcgactggcg ccactgccgc
     4921 ccgggcggtg cgccgctatc ggccgctcgc gccaaatcac cctccctgat gtcgttctgg
     4981 ctttatggta gcgcttccat cggcgatctt atagcttttg gcaatcgccg caatcgcccg
     5041 gcgcgggttt ctgcggacgg aggcgaggat gggcgggaac gcaggccagg gacaggtgcg
     5101 gatggtcgac gtggcgcggc gggccggggt cgccacggtc accgtctcgc gggtcctgca
     5161 cgaggccgaa aaggtggcac ccgcgacccg gtcccgggtc gaggcggcca tcgccgagct
     5221 cggctatctg ccgaatctcg tcgcccgctc gctggtggcg gcgcgcaccg gcatggtggc
     5281 ggccctcatc ccgaccatcg acaactcgct gcatgccgag atgatccagg gcatggacga
     5341 acgctgcgcc gctgccggcc tgcatctttt gatcggctgc acggccttct cgctcgaggt
     5401 ggaggagcgg atgctgcgcg ccttcctctc ccgccagccc gaggcggtct acctcaccgg
     5461 cagcaaccac agcccggcga cgcgcaccat gctgaaggcg tcgagggtgc cggtggtcga
     5521 ggtcgcgagc ctgcccgaga cgccgatcga catggtcgcc ggctattcca gctactgcgc
     5581 cgccgcggcg gtgctccgcc acctgatcga gcggggctac cggcggatcg gctacgtgta
     5641 ttcgccgatc ggcgtcaacg accggctggc cgagcggcac cgggcctatc tcgagacgct
     5701 ggcctcggcc gggctcgaga cgcccgaagc ctggcgcttc gagagcgagc tcggcatggc
     5761 cggcgggggg ctagcactgg cccggctccg ggcgcaggcg ccggagctgg acgcggtctt
     5821 ctgctccacc gacgtgctgg cggtcggcgt gctgctcgaa tgcctgcgcc agggcattgc
     5881 cgtgccgggc gagctcgccg tcgccggctt cgacgacctg cccatcgccg ccgagatcgt
     5941 gccggccttg accaccgtgc gcatcccgcg ccggcgcatg ggcgaggtcg cggccgggct
     6001 gatcctccga cgcctcgccg gcgaggcggt ggcggagccg gtcgtcgacc tgggcttcga
     6061 gctgcaggtg cgggccagcg cctgaggatg cccgtcgcgg gtcgaaggct tgccggttca
     6121 tcggggacga catagacgag cgggaggcga agctcggcga tgggacggag accaggatga
     6181 cgcgaacggt gctgatcacc ggtgcggccg ggcggatcgg gcggaaggtg gcagcgcacc
     6241 tcgaggcccg tggtggctat gcgctgcggc ggctcgatgt gcgggccgac gcgccggacg
     6301 tggtcgcggc cgatctggcg gattgggacg aaggctgggc gggggcgttc tcgggcgtcg
     6361 acacggtcct gcatctggca gcccacccca gccccgagat cgactgggcg accgcccagc
     6421 ggctcaatct cgacctcacc tacaacgtcc tgcacgcggc cgggaagcac cgggtgaggc
     6481 gcgtggtctt cgccagctcg aactgggtca tggccggcta tcgggaccag accggccccc
     6541 tgacgccgga catggcgccc ttgccggtca atccctatgg cgtcagcaag ctggtcggcg
     6601 agcgggcggg ccggagcctc gcggatcgcg gcatctcgtt catcgggctg cggatcggct
     6661 acagcgtcag cggcgagggg cggcccgcga gcgagctcaa gatggggctc tggggccggc
     6721 agatgtggct gagcgaccgg gacctctgcc agggcttcga gcggtcgatc ctggccgaag
     6781 gcgtgggctt taccgtgctc aacctcatgt ccgacaatcc ggggatgcgc tggtcgctgg
     6841 acgagacacg gcgggtgctc ggctacgagc cccaggacgg ctacccggcg gaaggctgag
     6901 gcacgcccag gcgtgtggct cgggcttggg cctctagcgc caggcgggtg acgcggacgg
     6961 cgtggccctc gggcatggcg gtggcggtgc ggtcccggac atcggcgagg aagcggcgga
     7021 aataggtcag tggctcgccc gaggcgtcga ggcggcgcgt gccctcgcca ttgaccagca
     7081 ggacgtggtc ggtgcccgcg ttgccggcga tgtcgatgta tttgcgcagc tcgagatagc
     7141 cttcggtgcc gagcaggaag agccggccgt cgccccaggt ggcgaggccg tcgggcgtga
     7201 accagtcgac ccggatgaag cccgaggcct tctccgagcg gagcaggatc tcgccgtaat
     7261 cctcgaagcc ctggatgccg tggtcgccga agctgccgac ggcgctggcg gcgatctcca
     7321 cctcgtcgct gccggtgaag aagaggaact ggtcgatctg gtgcgaggcg atgtcggcga
     7381 ggatgccgcc atgcgctgca tgctcgaaga accaggcggg gcggatggtt cggttgaggc
     7441 gatgcggccc gaggccggtg gtctggatca cccggccgat ggcaccctcg gcgaccagac
     7501 gggcggcgag ctcgcaggcc ggcacgatga agcgctcgga gaagcagatc gagaagatgc
     7561 ggccggtctc gcgggcggtc gcctcgaccc ggtcgagatc ggcgagcgtc gtgcagccgg
     7621 gcttgtcgac catcacgtcc ttgccggccc gcatcgcccg gatggcgagg gcggcccggt
     7681 cgcgcggcac ggcggcgctg cagatgatgc cgacgcccgg gtcatcgagg agcttttcgc
     7741 ggtcgacggc cggaagatcc ggaaagcgtt cccgaaagcc ttcgagcacg cgggggtcgg
     7801 tggtctttgg gcagtagccg gcgcagcgag caccggcggc gatcagctcg ccgaccatgt
     7861 ggtggatgtg gcgatggtcg aggccgatcg cggcgaagac gggcgtgtcg ctcatcggga
     7921 ggttcccggg ggtcgaggag ccccaaggtc ggggccggca cgagggtgcc tagcatccgc
     7981 accacgggga gggccagcgt ggcgacgacc cttgcctcag ccgccggcct ccttgccctc
     8041 ctcgatcttg tcccttgcct cctcgcggtc gttgccctcg ggcgccggcg cttcctgaac
     8101 cagctctgcc tcgcgctcga ggcagagctc gatcaggacc gggaagtgat ccgagccgta
     8161 atagccgagc cgccgcatct cgatcagggt gaaggtgtcg gtgtggaaga catggtcgag
     8221 cggccagcgc agcacggggt acctggcgtg gaacgtgctg aaccggccgc ggccgatgcg
     8281 cgggtcgagg gcaccgctgg tgcggcgaaa gaggcgggtg gtgtgcgacc aggccacgtc
     8341 gttgagatcg ccggcgacga tcgtcggccg gttgctctcc ttcatctcct tgccgacgat
     8401 caggatctcg gcgtcgcgct gctcggtgtc ctggccgggt cgcggcgggc gggggtgcag
     8461 gccgatgaag tcgatccact ggccggaacg aagccgcacg cgcgtgcgga ccgagggcac
     8521 gtcgtcctcg acgagaaagc ggatctcggg cgagtcgagg tcgagcctcg agaagagatg
     8581 caggccgtag gtgttgtcca gcgggcgggc caggccgaac gggtagctct cccggagcac
     8641 cgcgagctgc tcgtcccacc agtcgtcggt ctcgaccgtg aagatcaggt cggggtcggc
     8701 gcctcggacc agagcgagga agtcctccgc ccgccggttg tccatcagca cgttggcgat
     8761 catcagacga acccgcgagg ccggctcgca gtcgcggctc agcaggacct cgggggtcgc
     8821 gagcggcgtg tagggaaaga cccgccaggc ctggtaggcg agggtggcgg cgagggccag
     8881 aagaaaggcc gcgccggaca ggctcgccgg gctgaagagg atgagtgccg cgacgatcag
     8941 gccggccagg gcaatggcga gctggaggcg ggggaagtcg aagaagcgga tccaccactg
     9001 atcgctgcgg acgagcggtg ccaggctcgc gaggaccagc aggatgccga tgaccaggat
     9061 cgcggtcttc atcatcagga gccccgtctg agtcccgccg ccatgcagcc ggagctaggg
     9121 ccgggacagg cggggtcaac ccggcgccct tgtcctaagg gccgaagagc cacagcgggc
     9181 cgagggtgaa ggccggcacg aaggtgacca gcatcaggac caccaggagg gccaggatga
     9241 agggcacgac ggcacggaag acggcggtga catccgcctt gccgacccgg gcggtgatga
     9301 agaccagcat gccgagcggc ggggtcaggg caccgatgac gaggttcagg acgaggacga
     9361 tgccgaactg gatcgggtcg tagccggcgg ccttcaccat gggcaccagg atgggaacga
     9421 ggatcacgag ggccgcgatc atctcgatga agaggccgac gaagagcagg aagaggttga
     9481 tgagcagcag caggacgacg agcccggcgt cgagagagcc catggccgcg gccaggcgct
     9541 gcggcacctg ctcggcggcg agcacccagg cgaagggggc ggcggcg
//
LOCUS       DAOTFI010000190         9566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8505_length_9566_cov_6.837241, whole genome shotgun sequence.
ACCESSION   DAOTFI010000190 DAOTFI010000000
VERSION     DAOTFI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8505_length_9566_cov_6.837241"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="P5558_17450"
     CDS             <1..760
                     /locus_tag="P5558_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY26159.1"
                     /translation="GCSLRGGEGTIIGVVIGALPGTGGDIAALLAYDHAKRTVKNPSS
                     PFGEGAYEGLVGPESANNAAVGGAYIPMLTLGIPGDAVTAVIIGALYIHGLKPGPLLM
                     VETPHLFWFTVGSLTLANLCLLVFGLTGIRIFTKLVECPRGILIPLILVLSAVGTYAI
                     QNNPVDIYWMLAFGLLGYFMKVYGYPVAPVILGVILGPLMDVSYRRAMISARDDPLTF
                     LGEFLTAPISLGLTLFLLAIILGQTPLWAKLRGR"
     gene            complement(776..2461)
                     /locus_tag="P5558_17455"
     CDS             complement(776..2461)
                     /locus_tag="P5558_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY26160.1"
                     /translation="MNEPNGAEALVRLLELQGVRHVFGLCGDTSLPFYDAFQRLAPGI
                     EHILTRDERHAAYMADTYARLTGRPGICEGPSGGGATYILPGVVEAQETAVPLLAITS
                     DIATTSRGRFALTELDQVSLFRPLTKWNVTLDQAPRLPAMLRRAFREMTSGRPGAVHL
                     ALPFDTQKAPVPAAELYGDAAFTAVPASRPAADPAAVEALAKALAAAERPMAICGGGV
                     LTSGAEAALAALAERLNLTVATTITGKGSIGDDHPLALGVVGSNGGTAETRRLVDEAD
                     LILFIGARAGSVTTERWRHPQPGKALIAHVDVDPAVLGASYQTAHSVAGDARTVLEAL
                     LSALGDRPVRAADPRSAIAGAKAEKFARFEELAGSDERPIRPEVLVAAAQAALPADGV
                     IVADPGTPCPYFSAYWRQQGAGRRFISNRAHGALGYALPASIGAAFARPGAAVLSVMG
                     DGSFAMAQGELETALRLQVPVTFVVIANSSFGWIKAGQKSGFGGRYFSVDFSPCPAAA
                     IARAMGLDAVRVEAPGELEPALRSGLRSGRPNLIEVVAQPLEEAAAPVSEWVA"
     gene            2582..3184
                     /gene="wrbA"
                     /locus_tag="P5558_17460"
     CDS             2582..3184
                     /gene="wrbA"
                     /locus_tag="P5558_17460"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HRY26161.1"
                     /translation="MTRILVLYYSSYGHIETMAGAVAEGAASVDGVEVTVKRVAELMP
                     EEVAKKAGVKLDQAAPLATPAELADYDAIIVGTPTRFGNMAAQMRNFWDQTGGLWAKG
                     ALVGKVGSVFTSSNTQHGGQETTIISTHLTLLHQGLVIVGLPYSCAAQMGVDEIKGGS
                     PYGASTIAGNDGSRRPSEQELGMARYQGRHVAEIAKKLTA"
     gene            3198..4184
                     /locus_tag="P5558_17465"
     CDS             3198..4184
                     /locus_tag="P5558_17465"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY26162.1"
                     /translation="MGLLVDGQWQDRWYDTKASGGRFVRSDSQFRNWITADGSPGPTG
                     EGGFEAEPGRYHLYVSLACPWAHRTLIFRKLKGLERLIDVSVVHWHMAEHGWTFKTDE
                     AATGDRLFGLDYLHQLYTRAEPTYSGRVTVPVLFDKQRQTIVSNESAEIIRMLNSAFD
                     GVGATPGDYYPAPLRPVIDAINERVYDTVNNGVYKSGFATTQTAYEEAVAALFASLDW
                     LDDRLARQRYLAGDRVTEADWRLFTTLLRFDPVYVGHFKCNLRRLVDYPSLWAYAREL
                     HQWPGVAETVSMDHIKKHYYGSHGSINPTGIVPTGPVIDFDAPHGRERLAAA"
     gene            4192..4743
                     /locus_tag="P5558_17470"
     CDS             4192..4743
                     /locus_tag="P5558_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26163.1"
                     /translation="MAMARPRHRALGIVLVVLGGCATLDDGAWSAPRRPPLDITRDAV
                     GPTPGQVVGSWACHELDPFPDQAPVLTDLRLDPDGSFEWQQRILLDGETPGPFELVLI
                     LEGNWRLQGNRLVRSGVTGTSRPADGSGPAGPRPLMDGVLAAFARQAETVPTELLRVT
                     EGELVMRDVEAGVATLGCRRPAP"
     gene            4814..5359
                     /locus_tag="P5558_17475"
     CDS             4814..5359
                     /locus_tag="P5558_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26164.1"
                     /translation="MPNHVASALALVLAAGLLAACDPGDLNPLASEPRPPVSVTKEVT
                     MVSAAELVGSWTCRELNPYPDQPPIETQLDIRDDGTLTSEALLPMDQEMPGAGDLILT
                     IDADWQVEGDRMVTTNTQGSTRAADGSTGGVSDLLNQAMAAFTELAADGTSEVLKVDA
                     RELVMRGDEPDAPTVSCQRAS"
     gene            5379..6308
                     /locus_tag="P5558_17480"
     CDS             5379..6308
                     /locus_tag="P5558_17480"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26165.1"
                     /translation="MRVLVTGSSGWLGQTLVPLLEADGHAVTGLDLVPAPTTRVTGSI
                     TDRPLVFRTMAEAGIEAVIHAATLHKPHVATHSQSDFTAVNVQGTLNLLEAATASGST
                     VERFVLTSTTSLMISADIRAGRAGGATRAAWITEAMTGLRPRNIYGVTKLAAEHLCRL
                     THEQHGLPMVVLRTSRFFPEADDMAHAIPQSDTNTKANELLFRRLTVEDAAAAHLAAL
                     AKAPALGFDTFIVSAPTPFTPADCTGLITDAPAVVARYFPHYPEIYRRLDWTMFQSID
                     RVYDAARAARHLGFICRTGFAEKLAELAAGDST"
     gene            complement(6319..6804)
                     /locus_tag="P5558_17485"
     CDS             complement(6319..6804)
                     /locus_tag="P5558_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26166.1"
                     /translation="MRAGTALLWSMLAALPLAAQGQDVHVEWEDRCAECHGHAGAFAR
                     QRLEVVDGVLVSDHWGADLGRFLASHHTTPATLGPITAMLTAQATTPPVYAERCAGCH
                     GPAAGLVRQSIVRRNGQPVIASSGLPLADALERHGRLEPGELDIVVQTLDRLIDETIA
                     R"
     gene            complement(6801..6998)
                     /locus_tag="P5558_17490"
     CDS             complement(6801..6998)
                     /locus_tag="P5558_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26167.1"
                     /translation="MRPHPWFQPLYRRVLVMLFCAGWTAWEGYYDAGSMWFLLMLGVT
                     AWAAWDFFLSGNYAPKPASSE"
     gene            complement(7036..8418)
                     /locus_tag="P5558_17495"
     CDS             complement(7036..8418)
                     /locus_tag="P5558_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRY26168.1"
                     /translation="MPIAPNSLAARDIAYNVHPYTNLRVHEEKGPLVITGGEGIYVKD
                     DDGKEYIEGLAGLWCTSLGFSNKRLVEAARRQMDAMPYSHQFAHRSTEPVIDLAEKLI
                     GIAPKPLQKAFFVNSGSEAIDTAIKLIWYYNNARGKPEKKRIIARRRAYHGITVAAGH
                     LTGLPYARAGFDLPMSDRFFHVTPPTHYREGLPGESEAAFTDRLAQEVETLIQALGPD
                     TVAAFVAEPVQGAGGVIVPPADYFRKIQPILKKYDVLMVADEVICGFGRTGQMFGSNT
                     FGIEPDLMTVAKQLSSAYLPIAGLLMREAFYDVLAEQTQKLGVLGMGYTYGGHPAAAA
                     VALETLKIYEEDDVVGHVQAVSPRFMARLKQLGEHPLVGEARGVGLIGALEIVADKGT
                     RAQYPVAAKAAAAVATNCQARGLILRPLPGDVVGICPPLIITEREVDMLFDRLGEALD
                     DTLPAMQKAA"
     gene            8604..>9566
                     /gene="hemH"
                     /locus_tag="P5558_17500"
     CDS             8604..>9566
                     /gene="hemH"
                     /locus_tag="P5558_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRY26169.1"
                     /translation="MSEVEMIEKPTGRQAAAAATGPLPGGHPPVLPRRIGVVLLNLGT
                     PDATDYWSMRRYLGEFLSDKRVIDYPAWLWQPLLQLVILTKRPFTSGAAYRAIWNTER
                     DESPLRTVTRSQALKLAARFADRHPDVTVDWAMRYGNPSIRSVLDRMAAAGCDRIALM
                     ALYPQYAAPTTATAYDKAFEALMTMRRQPAIRTMGPYHDDPRYIELLARSIERHLATL
                     DWQPQRLIMSYHGVPKRFLMSGDPYHCMCHKTTRLVRERLGWDEERVMTCFQSRFGRE
                     EWLQPYFDQTLERLPKEGMKKIAVISPAFVTDCVETLEEIDQEGR"
BASE COUNT         1479 a         3570 c         3112 g         1405 t
ORIGIN      
        1 cgggtgcagc ctgcgtggcg gtgagggcac catcatcggc gtggtcatcg gtgccctgcc
       61 gggcaccggc ggcgacatcg cggcacttct ggcctacgac cacgccaagc ggaccgtgaa
      121 gaacccgtcg agccccttcg gcgaaggcgc ctacgagggt ctggtcggcc cggaatccgc
      181 caacaacgcc gcggtcggcg gcgcctacat cccgatgctg acgctaggca tcccgggcga
      241 cgcggtcacc gccgtcatca tcggcgccct ctacatccac ggcctgaagc caggcccgct
      301 gctgatggtc gagaccccgc acctcttctg gttcaccgtg ggctcgctga ccctggccaa
      361 tctctgcctg ctggtcttcg gcctcaccgg catccgcatc ttcacgaagc tggtcgaatg
      421 ccccaggggc atcctgatcc ccttgatcct cgtgctctcg gcggtcggca cctacgccat
      481 ccagaacaac ccggtcgaca tctactggat gctcgccttc ggccttctcg gctacttcat
      541 gaaggtctac ggctaccccg tggccccggt gatcctcggc gtgatcctcg gcccgctgat
      601 ggacgtctcc taccggcgcg ccatgatctc cgcccgcgac gacccgctca ccttcctcgg
      661 cgagttcctc accgccccca tctccctcgg cctcaccctc ttcctcctcg ccatcatcct
      721 cggccagacg ccactatggg cgaagttgcg tgggcggtga ggtgccgtct ctgcctcaag
      781 ccacccactc gctgaccggt gccgccgcct cctcgagcgg ctgggccacc acctcgatca
      841 gattcggccg gcccgagcgc aggccggatc gcaacgccgg ctcgagctcg cccggcgcct
      901 cgacgcgcac cgcatcgagg cccatggcgc gggcgatggc ggcggccggg caggggctga
      961 agtcgaccga gaagtagcgc ccgccgaagc cgctcttctg gcctgccttg atccagccga
     1021 agctgctgtt ggcgatcacg acgaaggtca ccggcacctg cagccgaagc gcggtctcga
     1081 gctcgccctg ggccatggcg aagctgccgt cgcccatgac cgagagcacc gcggcaccgg
     1141 gccgggcgaa ggcagcaccg atcgaggccg gcagggcata gccgagagcc ccgtgggcac
     1201 ggttgctgat gaaccgccgg ccggcgccct gctgccgcca gtaggccgag aaataggggc
     1261 agggcgtgcc ggggtcggcg acgatcacgc catccgcggg cagggccgcc tgcgcggccg
     1321 cgaccagcac ctcggggcgg atcggccgct cgtcgctccc ggccagctct tcgaagcggg
     1381 cgaatttttc cgccttggcg ccggcgatgg ccgagcgcgg gtcagcggcc cggaccggcc
     1441 ggtcgcccag ggccgaaagc agcgcctcca gcaccgtccg cgcgtcgccg gcgacgctgt
     1501 gcgcggtctg gtagctggcc cccagcacgg cggggtcgac atcgacatgg gcgatcaacg
     1561 ccttgcccgg ctgcggatgg cgccagcgct cggtggtgac cgagccggcc ctggcaccga
     1621 tgaagaggat caggtcggcc tcgtccacga gccgccgcgt ctccgccgtg ccgccgttcg
     1681 agccgaccac gccgagcgcc aggggatgat cgtcgccgat gctgcccttg ccggtgatgg
     1741 tggtggcgac ggtgaggttc aggcgctccg ccagggccgc gagcgccgcc tcggcaccgc
     1801 tggtcaggac accgccaccg cagatcgcca tgggtcgctc cgccgcggcg agcgccttgg
     1861 ccagggcctc gacggcagcg ggatcggcgg cgggccggct cgccggcacc gcggtgaacg
     1921 ccgcgtcgcc atagagctcg gccgccggca ccggcgcctt ctgggtgtcg aagggcaggg
     1981 ccagatgcac ggcacccggc cgccccgacg tcatctcgcg aaaggcccgg cggagcatgg
     2041 ccggcagccg gggcgcctgg tcgagcgtca cgttccactt ggtcagcggg cgaaaaagag
     2101 aaacctggtc cagctcggtc agcgcgaaac ggccgcggct cgtggtcgcg atgtcgctgg
     2161 tgatggcgag gagcggcacg gccgtctcct gggcctcgac cacgcccggc aggatatagg
     2221 tggcgccacc ccccgacggc ccctcgcaga tgcccggccg gccggtcagc ctcgcatagg
     2281 tgtcggccat gtaggcggca tggcgctcgt cccgggtgag gatgtgctcg atgccgggtg
     2341 ccagccgctg gaaggcgtca tagaagggca ggctggtgtc gccgcagagc ccgaagacat
     2401 ggcgcactcc ctgcagctcg agcagccgga ccagcgcctc cgccccgttc ggctcgttca
     2461 tcgatcgtcc tttcctgcca tcgttgccgg cggcgaaacg ctgccattcc gccgctcctc
     2521 ctgccgatcc cggaccggcg ccctatgtca tctcaatgcc attcatgggg agacgtgtcg
     2581 catgaccagg attctggtgc tctactacag ctcgtatggc catatcgaga ccatggccgg
     2641 ggccgtcgcc gaaggtgccg ccagcgtcga cggtgtcgag gtcacggtca agcgcgtggc
     2701 cgagctgatg ccggaggagg tggcgaagaa ggccggggtc aagctcgacc aggcagcccc
     2761 cctggccacc cccgccgagc tcgccgacta cgacgccatc atcgtcggca cgcccacccg
     2821 gttcggcaac atggcagcgc aaatgcgcaa tttctgggac cagaccggcg ggctctgggc
     2881 caagggcgcc ctggtcggca aggtcggcag cgtcttcacc agctcgaaca cccagcacgg
     2941 tggccaggag accacgatca tctccaccca cctcaccctc ctgcaccagg gcctcgtcat
     3001 cgtcggcctg ccctattcct gcgcggccca gatgggcgtc gacgagatca agggtgggtc
     3061 gccctatggc gcctcgacca tcgccggcaa cgacggcagc cgccggccct ccgagcagga
     3121 gctcggcatg gcgcgctacc agggccggca tgtcgccgaa atcgcgaaga agctgacggc
     3181 ctgaagggac agttgacatg ggattgctgg tcgacggcca gtggcaggac cgctggtacg
     3241 acacgaaggc atcgggcggg cggttcgtcc gctccgattc ccagttccgc aactggatca
     3301 ccgccgacgg cagccccggc cccacgggcg agggcgggtt cgaggcggag cccggccgct
     3361 atcacctcta cgtctcgctc gcctgcccct gggcgcaccg cacgctgatc ttcaggaagc
     3421 tcaagggcct ggagcggctg atcgacgtct cggtcgtcca ctggcacatg gccgagcatg
     3481 gctggacctt caaaaccgac gaggccgcca ccggcgaccg gctcttcggc ctcgactacc
     3541 tgcaccagct ctatacgagg gccgaaccaa cgtattccgg ccgggtgacg gtgccggtgc
     3601 tcttcgacaa gcagcgccaa accatcgtct ccaacgagtc ggccgagatc atccggatgc
     3661 tcaactcggc cttcgacggc gtcggcgcca ccccgggcga ctactacccg gcccccctcc
     3721 ggccggtgat cgacgccatc aacgagcgcg tctacgacac ggtcaacaac ggcgtctaca
     3781 agtcgggctt cgccacgacg caaaccgcct acgaggaggc cgtagccgcc ctcttcgcca
     3841 gcctcgactg gctcgatgac cgtctcgccc gccagcgcta cctcgccggc gaccgtgtca
     3901 ccgaggccga ctggcgcctc ttcaccacgc tcctccgctt cgatccggtc tatgtcggcc
     3961 acttcaagtg caatcttcgc cgcctcgtcg actacccgag cctttgggcc tatgctcgcg
     4021 agctccacca gtggcccggc gtggccgaaa cggtgagcat ggaccatatc aagaagcact
     4081 attacggcag ccacggcagc atcaacccga ccggcatcgt gcccacaggt cccgtcatcg
     4141 acttcgatgc gccgcacggc cgcgagcgcc tcgccgccgc ctgagcagcg catggcgatg
     4201 gctcggccgc gccatagggc actcgggatc gtcctcgtcg tgctcggcgg ctgcgccacg
     4261 ctcgacgacg gtgcctggag cgcaccgcgc cgcccgccgc tcgacatcac ccgcgacgcg
     4321 gtcggcccga ccccgggcca ggtcgtcggt agctgggcct gccacgagct cgatcccttc
     4381 cccgatcagg cccccgtcct gaccgatctc cggctcgacc ccgacggcag cttcgaatgg
     4441 cagcagcgca tcctgctcga cggcgaaaca ccgggcccgt tcgagctcgt cctgatcctc
     4501 gaaggaaatt ggcggcttca gggcaaccgc ctggtccgga gcggcgtcac cggcaccagt
     4561 cgccccgccg acggcagcgg cccggccgga ccccggccac tgatggacgg cgtcctcgcc
     4621 gccttcgcca ggcaggccga gaccgttccg accgagctcc tgcgcgtgac cgaaggcgag
     4681 ctcgtcatgc gcgacgtcga ggcgggtgtc gccacgctcg gctgccggcg cccggccccc
     4741 tagggagggg cttgccaagc tcacgcgcgc tcggcacctt cgcgccttgc cctgtcatcc
     4801 ggagagccac cccgtgccga accacgtcgc ttccgccctc gccctggtcc tcgccgccgg
     4861 cctgctcgcc gcctgcgacc cgggcgacct gaacccgctc gccagcgagc cccggccgcc
     4921 ggtgagcgtg acgaaggagg tcaccatggt ctccgccgcc gagctggtcg ggagctggac
     4981 ctgccgggag ctcaacccct atccggacca gccgccgatc gagacgcagc tcgacatccg
     5041 cgacgacggc acgctgacca gcgaggcgct cctgcccatg gaccaggaaa tgcctggcgc
     5101 cggcgatctg atcctgacca tcgatgccga ctggcaggtc gagggcgacc gcatggtgac
     5161 caccaacacc cagggctcca cccgcgccgc cgacggcagc accggcggcg tctccgacct
     5221 cctcaaccag gccatggccg ccttcaccga gctcgccgcc gacggcacca gcgaggtcct
     5281 caaggtcgac gcccgggagc tggtgatgcg cggcgacgag cccgacgcac cgaccgtctc
     5341 ctgccagcgc gccagctagg agcctgcccg cctcgcctat gcgagtcctg gtcaccggct
     5401 cctcgggctg gctcggccag accctggtgc cgctgctcga ggccgatggc cacgccgtca
     5461 ccggcctcga cctcgtgccc gcgcccacca cccgcgtcac gggctcgatc accgaccggc
     5521 ctttggtctt ccgcaccatg gccgaggccg ggatcgaggc cgtcatccat gctgcgaccc
     5581 tgcacaagcc gcatgtggcg acgcacagcc agtccgactt caccgccgtc aacgtccagg
     5641 gcacgctgaa cctgctcgag gccgcgaccg ccagcggctc gacggtcgaa cgcttcgtcc
     5701 tcacctcgac cacatccctg atgatctcgg ccgacatccg cgccggccgg gcaggcgggg
     5761 cgacgagggc cgcctggatc accgaagcca tgaccgggct caggccccgc aacatctacg
     5821 gtgtgaccaa gctcgccgcg gagcatctct gccgcctgac ccacgagcag cacggcctgc
     5881 cgatggtcgt gctccgcacg tctcgcttct tccccgaggc cgacgacatg gcgcacgcca
     5941 tcccgcagtc cgacaccaac accaaggcga acgagctgct cttccgccgc ctcaccgtcg
     6001 aggatgccgc ggcggcccac ctggcagccc tggcgaaagc acccgccctc ggcttcgaca
     6061 ccttcatcgt ctcggcgccg acgccgttca cccccgccga ctgcaccggg ctcatcaccg
     6121 acgcccccgc cgtggtcgcc cgctacttcc cccactaccc ggaaatctac cgccgcctgg
     6181 actggaccat gttccagagc atcgaccgcg tctacgacgc cgcccgtgcc gcccggcacc
     6241 tcggcttcat ctgccgcacc ggcttcgccg agaagctcgc cgagctggcg gccggcgatt
     6301 cgacctgaaa tccggcggtc agcgggcgat ggtctcgtcg atcagccggt cgagggtctg
     6361 gacaacgatg tcgagctcgc ccggctcgag acgcccgtgc cgttcgaggg catcggccag
     6421 aggcaggccg ctgctggcga tcaccggctg gccatttcgc cgcacgatgg actgccgcac
     6481 gaggccggca gccggcccgt ggcagccggc acagcgctcg gcatagaccg gcggcgtggt
     6541 ggcctgcgcc gtcagcatgg cggtgatggg cccgagcgtc gccggcgtgg tgtgatgact
     6601 ggcgagaaac cgcccgagat ccgcccccca atggtccgag acgaggacac cgtccacgac
     6661 ctcgagccgc tggcgggcga aggcgccggc gtggccgtgg cactcggcgc agcgatcctc
     6721 ccactcgaca tgcacgtcct gcccctgcgc ggcgagcggg agggccgcca gcatggacca
     6781 gagcagcgcc gtgccggccc tcactcgctg ctggccggct tcggggcgta gttgcccgag
     6841 aggaagaagt cccaggccgc ccaggcggtg acgccgagca tcaggaggaa ccacatcgag
     6901 ccggcgtcgt aatagccctc ccacgccgtc cagccggcgc agaagagcat caccagcacc
     6961 cggcgataga gcggctggaa ccagggatgc ggcctcatcg cccggttcct gccgcccgct
     7021 cgcccgagag gatgctcaag ccgccttctg catggccggc agcgtgtcgt cgagcgcctc
     7081 gccgaggcgg tcgaagagca tgtccacctc gcgctcggtg atgatcagag gcgggcagat
     7141 gccgaccacg tcgcccggca gcgggcgcag gatcagcccc cgcgcctgac aattggtggc
     7201 gacggcggcc gcggccttgg ccgccacggg gtactgcgcc ctggttccct tgtcggcgac
     7261 gatctcgagc gcgccgatga gcccgacgcc gcgcgcctcg ccgaccaggg gatgctcgcc
     7321 gagctgcttc agccgcgcca tgaagcgcgg ggagacggcc tggacatggc ccaccacgtc
     7381 gtcctcctcg tagatcttga gggtctcgag ggcgaccgcg gccgcggccg gatggccgcc
     7441 ataggtgtag cccatgccga gcacgccgag cttctgcgtc tgctcggcca ggacgtcgta
     7501 gaacgcctcg cgcatcaaga ggccggcgat cggcagatag gccgagctga gctgcttcgc
     7561 caccgtcatc agatcgggct cgatgccgaa ggtgttagag ccgaacatct ggccggtgcg
     7621 gccgaagccg cagatcacct cgtcggcgac catcagcacg tcgtatttct tcaggatcgg
     7681 ctggatcttc cggaaataat ccgccggcgg cacgatgacg ccgcccgccc cctgcaccgg
     7741 ctcggcgacg aaggccgcca ccgtgtcggg cccgagcgcc tggatcagcg tctcgacctc
     7801 ctgcgccaga cggtcggtga acgcggcctc gctctcgccg ggcagaccct cgcggtagtg
     7861 cgtcggcggg gtgacgtgga agaagcggtc gctcatcggc aggtcgaagc cggcccgcgc
     7921 gtagggcagc ccggtcaggt ggccggcggc cacggtgatg ccgtgatagg cgcggcggcg
     7981 ggcgatgatc cgcttcttct ccggcttgcc ccgggcgttg ttgtagtacc agatcagctt
     8041 gatcgcggtg tcgatcgcct ccgagcccga gttgacgaag aacgccttct gcaggggctt
     8101 cggggcgatg ccgatcagct tctcggcgag atcgatcacg ggctcggtcg aacggtgcgc
     8161 gaactggtgc gagtagggca tcgcatccat ctgccgcctt gccgcctcga ccagccgctt
     8221 gttgctgaag ccgaggctgg tgcaccagag acccgcgagg ccctcgatat actccttgcc
     8281 gtcgtcgtcc ttgacgtaga tgccctcgcc cccggtgatg accaggggac ctttctcctc
     8341 gtgcacccgc agattggtgt agggatggac gttgtaggcg atgtcgcggg cggccagcga
     8401 attcggggcg atcggcatga cggtgcatcc tgcttgagtg gcggccggct tttcatgcga
     8461 gtgtcgcagg cgccgcgcca cgggcaacgt gtcaggatac tctatccgtc aatcgctggc
     8521 gcgacaatcc gggccgcttg ccgctccacg cagggcgcct atgtgcgaac gggtgggtgc
     8581 aatgcaaaag tagaggtcgg cggatgagcg aggtcgagat gatcgaaaag ccgacgggcc
     8641 ggcaggcggc agccgccgcc accgggcccc tgcccggagg ccacccgccg gtgctccccc
     8701 gccggatcgg cgtcgtcctg ctcaatctcg gcacgcccga cgccaccgac tactggtcga
     8761 tgcgccgcta tctcggcgag ttcctctccg acaagcgggt gatcgactat cccgcctggc
     8821 tctggcagcc gctgctccag ctcgtgatcc tgaccaagcg cccgttcacc tcgggcgccg
     8881 cctaccgcgc gatctggaac accgagcgtg acgaaagccc gctgcgcacc gtcacccgca
     8941 gccaggccct gaagctggcg gcccgcttcg ccgaccggca ccccgacgtc accgtcgact
     9001 gggccatgcg ctacggcaac ccctcgatcc gctcggtgct cgaccgcatg gcggcggcgg
     9061 gctgcgaccg catcgcgctc atggcgctct acccgcaata cgcggccccc acgaccgcca
     9121 ccgcctacga caaggccttc gaggccctga tgaccatgcg ccgccagccg gccatccgca
     9181 ccatgggccc ctatcacgac gatccccgct acatcgagct cctggcccga agcatcgagc
     9241 ggcacctcgc caccctggac tggcagcccc agcgcctcat catgtcctat cacggcgtgc
     9301 cgaagcgctt cctcatgagc ggcgatccct atcactgcat gtgccacaag acgacgcgcc
     9361 tcgtgcgcga gcgcctcggc tgggacgagg aacgggtcat gacctgcttc cagtccaggt
     9421 tcggccggga ggaatggctg cagccctatt tcgaccagac cctggaacgg ctgccgaagg
     9481 agggcatgaa gaagatcgcg gtgatctcgc ccgccttcgt caccgactgc gtcgagacgc
     9541 tggaggagat cgaccaggag ggccgc
//
LOCUS       DAOTFI010000191         9459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8632_length_9459_cov_7.448214, whole genome shotgun sequence.
ACCESSION   DAOTFI010000191 DAOTFI010000000
VERSION     DAOTFI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8632_length_9459_cov_7.448214"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(739..972)
                     /locus_tag="P5558_17505"
     CDS             complement(739..972)
                     /locus_tag="P5558_17505"
                     /inference="COORDINATES: protein motif:HMM:NF018978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HRY26170.1"
                     /translation="MSLVGAIVTFCMTWWLFFLMALPFGSVPPEHPEPGHATSAPEKP
                     RLLLKVAVATALAGLATWGISLVIESGLVSLRP"
     gene            complement(983..1402)
                     /gene="mce"
                     /locus_tag="P5558_17510"
     CDS             complement(983..1402)
                     /gene="mce"
                     /locus_tag="P5558_17510"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRY26171.1"
                     /translation="MAAAFALGRLNHVAIAVPDIRAASETYRKLLGATISAIEDQPAH
                     GVQTVFIELPNTKIELLGVLGDASPIRAFLEKSPAGGIHHVCYEVADIEAARDHLVAQ
                     GARVLGDGKPRIGAHGKPVLFLHPKDFCGTLVELEEA"
     gene            complement(1402..3108)
                     /locus_tag="P5558_17515"
     CDS             complement(1402..3108)
                     /locus_tag="P5558_17515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRY26172.1"
                     /translation="MTSSLRFVALGGIGEIGLNLYLYELDGRWLMVDCGIAFADDRHP
                     GADIILPDIAFIEERKERLDGIILTHAHEDHLGAVPYLWERLGCPIYCTTFPAAVLRR
                     KLAEHYVKNPAAIHQVKNGERFTVGPFGCRLIHMTHSIPEASALAIDSPWGVVMHTGD
                     WKLDPAPMLGEPSDIALLEEIGAEGVLALVADSTNVLSPGTSGSEAEVRDSLRDLIAA
                     QPHAVALTTFASNVARLETAMLAAAEAGREIVVVGRSMHRMIEAAKECGYLKGIPPLR
                     DERDAAGLPRHRTLYLVTGSQGEPRAAMQRIATGNHQRIRLEAGDTAIFSSKIIPGNE
                     RTLYSLHNHLVELGVEVITEEDHFVHVSGHPCRDEMEQMYRWMKPKIAIPVHGEARHL
                     YAHARFAKSLGVEQALVVRNGDLVRLGPPPAKVESEVHTGRRVLETDELLDSGDELYR
                     SRRRLASHGTVVVGLVLDPYGSVLAAPQLTAIGAIDLSSFGNLEKAAADLVAEAVEDL
                     QDEDVLDDERVAQAVRGAVRQALSLPRHKRPIVEVQVTRLSPSSLASLEDEAGEARVG
                     AA"
     gene            complement(3105..3902)
                     /locus_tag="P5558_17520"
     CDS             complement(3105..3902)
                     /locus_tag="P5558_17520"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRY26173.1"
                     /translation="MLLLIDVGNTNTVFALYREREPLGQWRLSTARERTAEEYAALLI
                     QLLTIGGFDHKGVGGVVISSVVPQTVFPLRKMSRTFFGREAFVVGEDVAIPIGNKLIN
                     PKEAGADRLVNALAAVRRYVPPLMIVDFGTATTFDIVDDEGAFLGGIIAPGINLSIEA
                     LHRAAAKLPRIAVEPPPQVIGRSTVQAMQSGIFWGYVGLIEGLARRVQDEYGRSMTVI
                     ATGGLAPLFARHCKVVEHVDPDLTMIGLVEIYLLNREIIRAGKKATQ"
     gene            complement(3913..4653)
                     /locus_tag="P5558_17525"
     CDS             complement(3913..4653)
                     /locus_tag="P5558_17525"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRY26174.1"
                     /translation="MTSPRFAIEPYPSLASTNDLVRERALGGEPEGLVVRAGIQTRGR
                     GRHGRSWASPTGNLYCSMLLRPERPLAEAATLSLVIGLSLADTLAEEAPGPELKWPND
                     VMVGGAKLAGILLESVIVGAKAPVIVAGIGVNVATCPKDLPYPATSLAGLGVDLPPES
                     LLERFLEAFGRDYDCWRAGGFSALRERWLERAHGRGREARVRDGDRLIDGRFVDVDPE
                     GRLVIDTRTGRRLIHAGELFFPLAEATS"
     gene            complement(4667..6127)
                     /gene="nuoN"
                     /locus_tag="P5558_17530"
     CDS             complement(4667..6127)
                     /gene="nuoN"
                     /locus_tag="P5558_17530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRY26175.1"
                     /translation="MDSQPAFDVIPALPELLLFIAGLLLLVLGSLRERDAARLVTPLA
                     ILSLIVAAFVVIGGDKTAAPILGGHFVHDSFAAFLKVVILAGSAASILLAQGYLRNQD
                     LDRFEYPLLILFASLGMCMMVSANSLLALYMALELQSLPLYVMAAFNRDQLRSTEAGL
                     KYFVLGALASGMLLYGCSLVYGFTGTLSFTALADLLRPGETGVASLGIGALVGLVFVA
                     AGLAFKLSAVPFHMWTPDVYEGAPTPVTAFFAAAPKVAAVGLTLRVLMQPFGAVAGEW
                     QQIIVFISVASMLLGAFAAIGQTNIKRLLAYSGIGHIGFALVGLAAGTERGVYAVLIY
                     MTIYVVMTLGTFAAVLMMRRQGRYVETIDDLAGLSRRQPMLALAFAIFMFSLAGIPPL
                     AGFFGKLYVFMAAIEAGLTPLAVIGVLASVVGAYYYLRIVKLIYFDAPAEAFDRPDRV
                     ELQAVLGVCAVLVLGFILIPGPLLATAQAAAAALMP"
     gene            complement(6137..7639)
                     /locus_tag="P5558_17535"
     CDS             complement(6137..7639)
                     /locus_tag="P5558_17535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRY26176.1"
                     /translation="MSDWPLLSLVTFLPLVGAAFILLIRGDTAVVAQNSRSVALWTAL
                     VTFLLSLLVLGNFDSSQAGFQLVEKRSWLLGFGIDYHMGVDGISLWFVLLSTLLTVIV
                     IIGSWHSVQDRVKEYMIAFLVMDTLMVGVFCALDLVLFYIFFEGILIPMYLIIGIWGG
                     ARRIYASFKFFLYTLLGSVLFLVAILVMYLQAGTTDIPTLMATEFAPGLQRWLWLALF
                     ASFAVKVPMWPFHTWLPDAHVEAPTAGSVLLAGVLLKLGGYGFLRFSLPMLPDASAYF
                     APLVYTLSIIAVIYTSLVALMQTDMKKLIAYSSVAHMGIVTIGIFTPNALGIQGSIIQ
                     MLSHGIVSGALFMVVGVVYDRIHSRDIERYGGLAERMPAYALVFMIFMLASVGLPGTS
                     GFVGEVLVLVGIFQTNSWVALFATSGMVLGAAYMLWLYRRTIFGALTKDDLKLIKDMR
                     PNEVIAFAPLLILVFAMGIYPAFFLDPMAASVENLLVQIGASPATDLALH"
     gene            complement(7644..>9459)
                     /gene="nuoL"
                     /locus_tag="P5558_17540"
     CDS             complement(7644..>9459)
                     /gene="nuoL"
                     /locus_tag="P5558_17540"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRY26177.1"
                     /translation="GVAALCSVIGLFSTIGAEPFKVPLFTWINVATFEADWALRIDAL
                     SGVMMFVVSFISCLIHIYSIGYMSHDKSIPRFMSYLSLFTFAMLMLVTSDNLVQLFFG
                     WEGVGVASYLLIGFWYTKPSACAAAIKAFLVNRVGDIGLMLGIASCYLLFDSVQYDVI
                     FGAAGAMAGTTVIVFGAEFDALTLTAILLFIGAMGKSAQLGLHTWLPDAMEGPTPVSA
                     LIHAATMVTAGVFLVARFSPVFEHAPTALALVTFIGASTAIFAATIGLTQFDIKRVIA
                     YSTCSQLGYMFFACGVGAYSAGIFHLYTHAFFKALLFLGAGSVIHAMSDEQDMRKMGG
                     IWRKIPVTYTVMWIGTLALVGFPLTAGYFSKDAIIEAAWASHNSFHLYAFWMGVTAAV
                     LTAFYSGRLIFMTFHGECRASEHVQSHIHESPLVMTAPLGILALGALFAGMAGVGMIA
                     ADGGWWNGAIFTGPDNHVLHDLHEVGFWVKAAPFIAMVIGLGLSWLFYIRMPEMPARV
                     ASAFRPLYLFFLNKWYFDELYDRIFVQPAKAIGYGLWRGGDMGIIDRFGPDGVAASAW
                     STAKRTARLQTGFVYHYAFAMLIGVALLVTWYLLVGRG"
BASE COUNT         1777 a         3002 c         3361 g         1319 t
ORIGIN      
        1 aacctacgtg agcaccgatc gtccgcagcc tgacgaagta tacggtcacc gtgaaaacgc
       61 tccgggcgtg gtcccgccca gcgcctgcat ccccgccgtc gcgtccgcgc cggccatcac
      121 cggacgggtc gggcattgac tgccggcaaa ttcccgctcg accgacgaaa tggcgaccac
      181 atggcgataa atggcagtga ttttcacggg gccgacgacc acaattgccg aacggcgccg
      241 cattttcccg agaaatgccg ccgaagccct ccgcctaccc gttcgccgcc tctcgggatg
      301 gcgcgtcgga tgcgacattc gcggtcgccg aacccgggaa tgtcgcggaa tctgcgacat
      361 ggttatgaca aatgtcgcgg attctgcaac atcccccctc gggttgtcgc gtattccgcg
      421 acacgattat gacaaatgtt gcggattccg cgacagttcc ggacgaacga acccgccggg
      481 cctgcgaacg aacccaggga ttcgacgaac gaacctgccg attcgacgat cgaacccaaa
      541 gaaatgcgaa cgaacccgac gagcgaccaa cgaacccgag ggccgccggc gagccccgac
      601 gcggctttcg ccgcccttgc gggcgcggag acgggccaga atcgctgatt tgccgatcga
      661 acccgaaaga cgtctggaca ggaatgtcga gctaggctat aaatcctaaa gacgtatcga
      721 aggcggcccc tcgttccgtc aggggcgcag cgagacgagg ccgctctcga tgaccagcga
      781 gatgccccag gtggcgaggc cggcgagggc cgtggcgacc gccaccttga gcagcaggcg
      841 cggcttctcc ggggcgctgg tggcatggcc gggctccggg tgctcgggcg gcaccgagcc
      901 gaagggcagg gccatgagga agaagagcca ccaggtcatg cagaaggtga cgatggcgcc
      961 gacgaggctc acgggctctc cctcaggcct cttccagctc gaccagcgtg ccgcagaaat
     1021 ccttgggatg caggaagagc acgggcttgc cgtgcgcccc gatcctgggc ttcccgtcgc
     1081 ccagcacgcg cgcgccctgg gccacgaggt ggtcgcgggc cgcctcgatg tcggccacct
     1141 cgtagcagac atgatggatg ccgccggccg ggctcttctc gaggaaggcc cggatgggcg
     1201 aggcgtcccc aagcacgccg agcagctcga tcttggtgtt cggcagctcg atgaagacgg
     1261 tctgcacccc gtgcgccggc tggtcctcga tggccgagat cgtggccccc agcagcttcc
     1321 tgtaggtctc gctggcggcc cggatgtccg gcaccgcgat ggcgacgtgg ttcagccggc
     1381 cgagggcgaa ggccgccgcc atcaggcagc gccgacgcgg gcctcgcccg cctcgtcctc
     1441 gagactggcg agcgaggagg ggctcagccg cgtcacctgg acctcgacga tcggccgctt
     1501 gtggcgcggc aggctgagcg cctggcggac ggcgccgcgc accgcctggg ccacccgctc
     1561 gtcgtcgagc acgtcctcgt cctgcaggtc ctcgaccgcc tcggcgacca ggtcggccgc
     1621 ggccttctcc agattgccga agctcgagag atcgatggcg ccgatcgccg tgagctgcgg
     1681 ggccgcgagg acgctgccat aggggtcgag gacgaggccg acgaccaccg tgccgtggct
     1741 ggcgaggcgg cggcgcgagc ggtagagctc gtcgccggaa tcgagcagct cgtcggtctc
     1801 gagcacccgc cggccggtgt ggacctcgct ctcgaccttg gccggtggcg ggccgaggcg
     1861 cacgaggtcg ccattgcgca cgaccagcgc ctgctcgacg ccgagcgact tggcgaaccg
     1921 cgcatgggcg tagagatggc gggcctcgcc atgcaccgga atggcgatct tcggcttcat
     1981 ccagcggtac atctgctcca tctcgtcgcg acagggatgg cccgagacat ggacgaaatg
     2041 gtcctcctcg gtgatcacct cgacaccgag ctcgaccaga tggttgtgca ggctgtagag
     2101 cgtgcgctcg ttgccgggga tgatcttcga ggagaagatc gccgtgtcac cggcctcgag
     2161 gcggatgcgc tggtggttgc ccgtggcgat gcgctgcatg gcggcgcgcg gctcgccctg
     2221 gctgccggtg acgagataga gggtgcggtg gcgcggcagg ccggccgcgt cgcgctcgtc
     2281 gcgcaagggc gggatgccct tcaggtagcc gcattccttc gcggcctcga tcatccggtg
     2341 catcgagcgg ccgacgacga cgatctcccg gcccgcctcg gccgcggcca gcatggcggt
     2401 ctccagccgg gcgacgttcg aggcgaaggt ggtcagcgcc accgcatgcg gctgggccgc
     2461 gatgagatcg cgcaaggaat cgcggacctc ggcctcggag ccggacgttc ccgggctcag
     2521 cacattggtg ctgtcggcga cgagcgccag caccccctcg gcgccgatct cctccagcaa
     2581 cgcgatgtcg ctcggctcgc ccagcatcgg tgccgggtcg agcttccagt cgccggtgtg
     2641 catcaccaca ccccaggggc tgtcgatagc gagcgcgctg gcctccggaa tggaatgggt
     2701 catgtggatc aggcggcagc cgaaggggcc gacggtgaag cgctcgccgt tcttgacctg
     2761 gtgaatggct gccgggttct tcacgtaatg ctcggcgagc ttgcggcgga ggaccgccgc
     2821 cgggaaggtc gtgcagtaga tcgggcagcc gagccgctcc cagagatagg gaaccgcacc
     2881 cagatggtcc tcgtgggcat gcgtcaggat gatgccgtcg agccgctcct tccgctcctc
     2941 gatgaaggcg atgtcgggca ggatgatgtc ggcgccggga tggcggtcat cggcgaaggc
     3001 gatgccgcaa tcgaccatca gccagcggcc gtcgagctcg tagagataga ggttcagtcc
     3061 gatttcgccg atgccgccga gggcgacgaa gcgcaaggat gaagtcactg ggttgccttt
     3121 tttccggcgc gaatgatttc gcgattcagg agatagatct cgaccaggcc gatcatcgtg
     3181 agatcggggt cgacatgctc caccactttg caatgccggg cgaacaacgg ggcgaggccg
     3241 ccggtcgcga tgacggtcat gctcctgccg tactcgtcct ggacccgtcg ggcaagccct
     3301 tcgatgaggc cgacatagcc ccagaagata ccggactgca tcgcctggac ggtcgaccgg
     3361 ccgatcacct ggggcggcgg ctcgacggcg atcctgggca gcttggcggc ggcgcggtgc
     3421 agcgcctcga tcgagaggtt gatccccggg gcgatgatgc cgcccaggaa ggcgccctcg
     3481 tcgtcgacga tgtcgaacgt ggtggcggtg ccgaagtcga cgatcatcaa aggcggcacg
     3541 tagcggcgca cggcggcgag cgcgttgacc aggcggtcgg cgccggcttc cttcgggttg
     3601 atgagcttgt tgccgatcgg gatcgccacg tcctcgccga cgacgaaggc ctcccggccg
     3661 aagaacgtcc ggctcatctt gcgcaaggga aagaccgtct gcggcacgac cgacgagatg
     3721 acgacgccgc ccacgccctt gtgatcgaag ccgccgatgg tcaagagctg gataaggagc
     3781 gcggcatatt cctcggcggt gcgctcgcgc gcggtcgaga gccgccactg gccgagcggc
     3841 tcgcgctccc ggtagagggc gaagacggtg ttggtgttgc cgacgtcgat caggagcagc
     3901 atgggcggct cctcacgacg tcgcttcggc aagcgggaag aagagctcgc ccgcatggat
     3961 cagccggcgt cccgtccgcg tgtcgatcac cagccggccc tccggatcga cgtcgacgaa
     4021 gcgcccgtcg atcagccggt cgccgtcccg cacccgggct tcccggccgc gcccatgcgc
     4081 ccgctcgagc cagcgctccc gaagcgccga gaatccgccc gcgcgccagc aatcgtagtc
     4141 ccgaccgaac gcctcgagga accgctcgag caggctctcg ggcgggaggt cgacgccgag
     4201 gcccgcgagg ctggtcgcgg ggtagggaag gtccttcggg caggtggcga cattgacgcc
     4261 gatgccggcg acgatgacgg gtgccttggc gccgacgatg acgctctcga gcagaatgcc
     4321 ggcgagcttg gcaccgccca ccatcacgtc attgggccac ttgagctccg gtccgggcgc
     4381 ttcctcggcg agcgtgtcgg cgaggctgag gccgatgacc agcgacagcg tcgccgcctc
     4441 ggcgagtggc cgttcggggc gcagcagcat cgagcaatag aggttgcccg tgggcgaggc
     4501 ccagctccgg ccgtgccgcc ctcggcccct ggtctggatc ccggcccgga ccacgagccc
     4561 ctcgggctcg ccaccgagcg cacgctcgcg gaccaggtcg ttcgtgctgg cgaggctcgg
     4621 gtagggctcg atcgcgaagc gaggactggt caatcggcct tatgccctat ggcatgagcg
     4681 ctgccgcggc ggcctgggcc gtggcgagca aggggcccgg gatcaggatg aagccgagca
     4741 ccaggacggc gcagacgccc aggaccgcct gcagctcgac gcggtccggt cggtcgaacg
     4801 cctcggccgg cgcatcgaaa taaatcagct tgacgatccg gagatagtag taggcaccga
     4861 ccacgctggc cagcacgccg atcacggcga gcggcgtcag gcccgcctcg atcgccgcca
     4921 tgaagacgta gagcttgccg aagaagccgg cgagcggcgg aatgccggcg agcgagaaca
     4981 tgaagatggc gaaggcgagg gccagcatcg gctgccgccg cgacagcccc gccaagtcgt
     5041 cgatggtctc gacatagcgg ccctgccgcc gcatcatgag gaccgcggcg aaggtgccga
     5101 gcgtcatgac gacgtagatg gtcatgtaga tcaacacggc gtagacgccg cgctcggtgc
     5161 cggccgcgag gccgaccagc gcgaagccga tatggccgat gccggaatag gcgagcaggc
     5221 gcttgatgtt ggtctggccg atcgcggcga aggcgccgag cagcatcgac gccaccgaga
     5281 tgaagacgat gatctgctgc cactcgccgg cgacggcacc gaagggctgc atcagcacgc
     5341 gcagcgtcag cccgaccgcc gcgaccttgg gggccgcggc gaagaaggcc gtcaccggcg
     5401 tcggcgcacc ctcgtagacg tcgggcgtcc acatgtggaa gggcacggcc gaaagcttga
     5461 aggcgaggcc ggcggcgacg aagacgaggc cgaccagggc accgatgccg agggaggcca
     5521 cgcccgtctc gcccggccgg agcaggtccg ccagggcggt gaacgacagg gtgccggtga
     5581 agccgtagac cagcgagcag ccgtagagca gcatgccgga ggcgagcgct cccaggacga
     5641 aatacttcag ccccgcctcg gtcgacctga gctggtcgcg attgaacgcc gccatgacat
     5701 agagcggcag gctctgcagc tcgagcgcca tgtagagggc gaggaggctg ttggccgaga
     5761 ccatcatgca catgccgagg ctggcgaaca ggatcaagag cggatactcg aaacggtcca
     5821 gatcctggtt ccgcaggtag ccctgcgcca ggaggatgct ggcggcggag ccggccagga
     5881 tgacgacctt gagaaaggcc gcgaagctgt catggacgaa atggccgccg agaatcggtg
     5941 ccgcagtctt gtccccgccg atgaccacga acgcggcgac gatgagcgac aggatggcga
     6001 gcggcgtcac cagccgggcc gcatcgcgct cgcgcagcga gcccaggacg agcaggagca
     6061 ggccggcaat gaacagcagc agctccggga gcgccgggat cacgtcgaag gctggctggc
     6121 tatccatcgg cgggcctcag tgcagggcca gatcggtggc cgggctggcg ccgatctgga
     6181 cgagtaggtt ctcgaccgac gcagccatcg ggtcgaggaa gaaggcggga tagatgccca
     6241 tggcgaagac caggatgagc agcggcgcga aggcgatcac ctcgttcggc cgcatgtcct
     6301 tgatcagctt caggtcgtcc ttggtcaggg cgccgaagat ggtccgccgg tagagccaga
     6361 gcatgtaggc ggcgccgagc accatgccgg aggtggcgaa gagggccacc cacgaattcg
     6421 tctggaagat gccgaccaac accagcacct cgccgacgaa gccggacgtg ccgggcaggc
     6481 cgaccgaggc gagcatgaag atcatgaaga ccagcgcgta ggccggcatg cgttccgcga
     6541 ggccgccata gcgttcgatg tcacggctgt ggatccggtc gtagaccacg ccgaccacca
     6601 tgaagagggc gcccgagacg atgccatggc tgagcatctg gatgatcgag ccctggatgc
     6661 cgagcgcatt gggcgtgaag atgccgatgg tgacgatgcc catatgcgcc accgacgaat
     6721 aggcgatcag cttcttcatg tcggtctgca tcagggccac gagcgacgtg tagatcacgg
     6781 cgatgatgga gagggtatag accagcgggg cgaaataggc cgaggcgtcc ggcagcatcg
     6841 gcagggagaa gcgcaggaag ccgtagccgc cgagcttcag cagcacgccc gccagcagca
     6901 ccgatcccgc cgtcggcgcc tcgacatgcg cgtcgggcag ccaggtgtgg aacggccaca
     6961 tcggcacctt gacggcgaag gacgcgaaca aggcgagcca gagccagcgc tgcaggcccg
     7021 gcgcgaactc ggtcgccatg agggtcggga tgtcggtcgt gccggcctgc aggtacatca
     7081 ccaggatggc gacgaggaag agcaccgagc cgagcagcgt gtagaggaag aacttgaacg
     7141 aggcgtagat gcgccgcgca ccaccccaga tgccgatgat caggtacatc gggatcagga
     7201 tgccctcgaa gaagatgtag aagagcacca gatcgagcgc gcagaagacg ccgaccatca
     7261 gcgtgtccat gaccaggaag gcgatcatgt actctttcac gcggtcctgg acggagtgcc
     7321 acgagccgat gatgacgatc acggtgagca gggtcgagag cagcacgaac cagagcgaga
     7381 tgccgtcgac gcccatgtgg tagtcgatgc cgaagcccag gagccagctc cgcttctcca
     7441 cgagctggaa gcccgcctgg ctcgaatcga agttgccgag gacgagcagc gagaggagga
     7501 acgtcaccag cgccgtccag agcgccacgc tgcggctgtt ctgggccacg accgcggtat
     7561 cgccgcggat caggagaatg aaggcggcac cgaccagggg caggaaggtg acgagggaaa
     7621 gcaggggcca gtcgctcatc ggctcaaccc cgcccgacca ggagatacca ggtgacgagg
     7681 agcgccaccc cgatcagcat ggcaaaggca tagtggtaga cgaagccggt ctgcaggcgg
     7741 gccgtgcgct tggcggtcga ccaggcgctc gccgccacgc cgtccgggcc gaagcggtcg
     7801 atgatgccca tgtcgccgcc gcgccagagc ccgtagccga tcgccttcgc cggctggacg
     7861 aagatccggt catagagctc gtcgaaatac cacttgttga ggaagaagag gtagagcggg
     7921 cggaaggccg aggcgacgcg ggccggcatc tccggcatcc ggatgtagaa gagccaggag
     7981 aggccgaggc cgatgaccat cgcgatgaag ggggccgcct tcacccagaa gccgacctcg
     8041 tgcaggtcgt gcaggacgtg gttgtcgggc ccggtgaaga tcgcgccgtt ccaccagccg
     8101 ccgtcggcgg cgatcatgcc gaccccggcc atcccggcga agagcgcacc cagcgccagg
     8161 atgccgagcg gcgccgtcat caccagcggg ctctcgtgga tgtggctctg gacgtgctcg
     8221 gacgcccggc actcgccgtg gaacgtcatg aagatcagcc ggccggaata gaacgccgtc
     8281 aggaccgcag cggtgacgcc catccagaag gcatagaggt ggaagctgtt gtgcgaggcc
     8341 caggcggcct cgatgatggc gtccttggag aagtagccgg cggtgagcgg gaagccgacg
     8401 agcgccagcg tgccgatcca catcaccgtg taggtgaccg ggatcttgcg ccagatgccg
     8461 cccatcttgc gcatgtcctg ctcgtccgac atggcatgga tgaccgagcc ggcgccgagg
     8521 aacaggagcg ccttgaagaa ggcgtgggtg tagaggtgga agatgccggc cgagtaggcg
     8581 cccacaccgc aggcgaagaa catgtagccg agctgcgagc aggtcgagta ggcgatcacc
     8641 cgcttgatgt cgaactgcgt caggccgatg gtcgcggcga agatcgccgt cgaggcaccg
     8701 atgaaggtca cgagcgccag cgccgtcggg gcatgctcga agaccggcga gaagcgggcc
     8761 acgaggaaga ctccggccgt caccatggtg gcggcgtgga tcagcgccga gacgggtgtg
     8821 gggccctcca tggcgtccgg cagccaggta tgcaggccga gctgggccga tttgcccatc
     8881 gccccgatga agagcaggat ggccgtcagc gtgagcgcgt cgaattcggc gccgaagacg
     8941 atgacggtgg tgcccgccat ggcgcccgcg gcgccgaaga tgacgtcgta ctggaccgaa
     9001 tcgaagagca ggtagcagct cgcgatgccc agcatgagcc cgatgtcgcc gacccggttg
     9061 accaggaatg ccttgatcgc ggcggcgcag gccgagggct tcgtgtacca gaagccgatc
     9121 aggagatagg aggcgacgcc gaccccttcc cagccgaaga agagctggac caggttgtcg
     9181 gaggtgacga gcatcagcat cgcgaaggtg aagagcgaga ggtagctcat gaaccgcggg
     9241 atgctcttgt cgtggctcat gtagccgatc gagtagatgt ggatcaggca gctgatgaag
     9301 ctgaccacga acatcatcac gccggagagc gcatcgatgc gcagcgccca gtcggcctcg
     9361 aaggtggcga cattgatcca ggtgaagagc ggcaccttga agggctcggc gccgatggtc
     9421 gagaagaggc cgatcaccga gcacagggcg gcaacgccc
//
LOCUS       DAOTFI010000192         9397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8719_length_9397_cov_6.995718, whole genome shotgun sequence.
ACCESSION   DAOTFI010000192 DAOTFI010000000
VERSION     DAOTFI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8719_length_9397_cov_6.995718"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="P5558_17545"
     CDS             <1..514
                     /locus_tag="P5558_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY26178.1"
                     /translation="TIRMLAGEIDGSIAGFLRGQVSVCVILGLFYGIGLAVIGLSGGL
                     LVGLLAGLLSFIPYVGSITGFVASMGLAAVQFGPTNFLMLGLTAGIFFVGQAVEGNFL
                     TPKLVGDRIGLHPVWVIFALLAGGALFGFLGVMLAVPVAAAIGVLVRFGLRQYLASPL
                     YKGHPLDKAD"
     gene            complement(511..1437)
                     /locus_tag="P5558_17550"
     CDS             complement(511..1437)
                     /locus_tag="P5558_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRY26179.1"
                     /translation="MSTAPIDREPVAIVGSGLIGAGWAAVFTAAGLEVRAWDPEPSRL
                     AGLRARVRPLRAALKKLQPVRRGRLVLAKSLEEAVAGVRLVQENAPEDIAIKRALFAR
                     IEAAAEPATVIASSTSALLWSDLAEEMARPDRLLVAHPFNPVHLIPLVELFTPDPALA
                     DHATAFYTALGKRPVRLRREMPGHIAGRLAAALWREAVHLVREGVASVADIDTALVDG
                     PGLRWAFQGAHLSYHLGGGAGGIADYLAHLGPSQERRWATLGQPRLDAEARERLVAGV
                     AEEARGRSVAELEAERDAALVELMRARRRAIS"
     gene            complement(1434..3371)
                     /locus_tag="P5558_17555"
     CDS             complement(1434..3371)
                     /locus_tag="P5558_17555"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY26180.1"
                     /translation="MALDRVTFEVLKNAYGNLVDQMSEQLLRTCYSFVIYCRDFSNCL
                     CDAEGNTVMQGSGDIAVHVGTLHFTCKSVIDRFKGDIHPGDVFISNDPYTGGTHFCDV
                     RIVRPVFVEGELIAFTQSNGHWADIGGSTPGSFDINARTHFGEGLRITPTRVWTKGQL
                     LEDVVEMIVGNTRASADAMGDFLAQCEATRLGEQRLLELVEKYGKDTVLEAFRESQDY
                     VERFARDKVAGLPDGTWTTTDFIDQDPTKPEGLIPIQVKMTIEGSQVSYDFAGSHDAI
                     DCFLNGSASSAFSAVVAGTKTFFPEVPLNSGFYRFIDADLPLGSVVNAPYPYAVTGFC
                     SGAYEKIMNAIFELWSRVMPERALACSFNLEYLLIGGLDDRPERRGNFFMWYDWMAGG
                     WGARNGKDGSGATSSIFGVGLAQQSVEAQERLTPILMDRYEIAADSAGPGEFRGGCGL
                     NKGGTITAIKSTVMSYCCDRARSVTWGMMGGQPSAPHGVTHTRGNESRFLGAVFSNVP
                     LQPGDRFDRPSAGGGGLFDPLKRDTARVLDDVIDGYVSIEGAARDYGVVVEAVDPELD
                     DYRIDEAATAKLRDELRQGQGGWLEEPAEAVAGRLRKGEIGMLEAIRRHGIICDWNDG
                     TLLPRSTEQYRAAAKIRARAA"
     gene            complement(3385..5448)
                     /locus_tag="P5558_17560"
     CDS             complement(3385..5448)
                     /locus_tag="P5558_17560"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRY26181.1"
                     /translation="MTQQAEGGSRRYRVAIDTGGTFTDAYFFDEATGAISVTKVPSRP
                     DQPDAAVMASIEAAGITPAEIALLTHGTTVATNALITRKLPKAAIVATRGFRDIVEIR
                     DGTKPDLWDAYVDVAPPYIRRRDRFEVTERVDYKGKVVKPLDEAEVRDLARLLKKRGY
                     RAVAVCFMNSYANPEPEQRCKAILEEALGPDVFVCASAEVVPEMFEHPRHSTAMINAV
                     TGPVVGRYIEALDRSLKSAGYAGDLLILHSGGGVLTAAGASRFAARLASSGIVAGAIA
                     GAHIAKQCGFENAISLDMGGTSTDISLCYKGVLPTTQRWHVEYGYPIMFPSIEVITIG
                     AGGGSIAWIDPGGSLRNGPQSAGADPGPACYGTGGTEPTNTDANVLLGRLGTSLLDGK
                     VALDGEAAKAAVTGTVGDRLGIDQTEAADAVVRVANANMANAVKLISVGRGFDPRDFA
                     LVVFGGAGPLHGADLARDLDIPVVIFPRFPGIASAMGCLLVDIRHDFATMFLKGASSE
                     TLDEVEAEFHKLEAEAKDRLREEGVAPGDMHLQRAFEMRYVGQWRQLTIDVESAKLRS
                     LDDVLDRFHQEHARAYSFEDRGRTVEIYSLRVVARGVVPKPRPAPASQAGGSVPEAAA
                     RRPVHFGKEHGFVETPVLRRDVLEAGMVLEGPMVIEQLDSTVVLPPGTRSEVTPDLHI
                     VMRFT"
     gene            complement(5476..6204)
                     /locus_tag="P5558_17565"
     CDS             complement(5476..6204)
                     /locus_tag="P5558_17565"
                     /inference="COORDINATES: protein motif:HMM:NF003816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HRY26182.1"
                     /translation="MTVVNCDMGEGYGLYQIGDDAGLMPHIHVANVACGFHASDFNHM
                     RNTVRLAKQHGVKVGAHPSLPDRQGFGRREMKIGREELANCLIYQIGALKGFLDAEGL
                     ELNHIKPHGSLYGMAARQEDIAHAVCDAADVYKVPVYGIINSLHETIYPARGHRFVAE
                     YYADLDYSDEGLMIITREHHAVDPARAAERCVRAIREGKTASVNGKDVPVRADSICVH
                     SDTPNAVAVAAAVAAAVQPYVDAA"
     gene            complement(6234..7238)
                     /locus_tag="P5558_17570"
     CDS             complement(6234..7238)
                     /locus_tag="P5558_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26183.1"
                     /translation="MAEAKSTGAARPAGVAWTALLVQALLPIALILLLLGFALVESRV
                     LSAGNLLNLLVQASYLAIFASAQMMVIITRGFDLSLGTCVSAVSVVSALAMTGSLAAG
                     GEATGLAIAAGMLFGLGMGALIGLFNGFAVAWLRVNPFVATLGSLNICLGIATMASDG
                     RPVFNVPREFSALVYNGSVMGVPMPVVLAVLLLAVLHFVLNHTVFGRALFLIGGNPTA
                     AAVAGLPSRRYLTLAYVLCSLIAAVGALMLTGRTGSGEPNLGGNLTLESIAAAVIGGV
                     SLRGGIGGVPSVILGALFVTVLSNGMNLTRIDGNVQMIVLGSVIILAVFLDRFRAVAR
                     "
     gene            complement(7231..8256)
                     /locus_tag="P5558_17575"
     CDS             complement(7231..8256)
                     /locus_tag="P5558_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26184.1"
                     /translation="MTVRPLAVHAHPPTGAEQLSQRLLSVGLLPILLVLSVAFMAVFE
                     QKFFGLNNLFNVLRSTSFLAIVAAGQMLVLIVGGFDLSVGAVIALTSTVMAKSMAALS
                     AAMPDQTAAIITLGVLAGLGCGVFIGLINGLCVAFLRVSPFMVTLGTLSIATGLALLL
                     TQGIPVYGMPDVFVKEFGRALWLGLPTTVYIALAIIALVWFMQTRTRLGRYLYAVGGN
                     LQAAIVSGVPATLNVILAYVLCSVLAATAGVLMTARLGSGQASMGGDDMMLQSIAAAV
                     IAGVSLRGGIGRVELVALGALFLSILTNAMNLLRVDSKMQVVVLGFILVLAVALEEYA
                     RRRRRHG"
     gene            complement(8253..>9397)
                     /locus_tag="P5558_17580"
     CDS             complement(8253..>9397)
                     /locus_tag="P5558_17580"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY26185.1"
                     /translation="TIADLGFALEPGRKVVELSRAHQQMTEIAKALMGNVRVLILDEP
                     TASLTESETVRLFELIAQLKASGIGIIYVSHRMSEIKQLGDRITVLRDGRLIATLDAA
                     GVSENELVELMTGRKIDLLFPHVDHRPAETLLQVEDLTLADHSVEGVDFHARAGEITG
                     IAGLVGCGKSELIRAVYGLETIIGGTIRVEGQAYGHPSPARSLERGVCYFPSDRVVEG
                     LALGRPIRENVSMAALDLTRFARLRIMQRGSERRIVQGIVDQLGLRPPNIERVVGNLS
                     GGNRQKVMLARGLTRTTRVFLFDEPTVGIDVGAKVEVYELMKQLVDSGAAIVLVSSEL
                     PEVLHLANRLYVMHRGRMVAELTGADIAEQRVLACFFQDEKTGQAA"
BASE COUNT         1516 a         3171 c         3303 g         1407 t
ORIGIN      
        1 caccatccgc atgctggccg gcgagatcga cgggagcatc gccggcttcc tgcgcggcca
       61 ggtctcggtc tgcgtgatcc tcggcctctt ctacggcatc ggcctcgccg tcatcggcct
      121 gagcggcggc ttgctggtcg gcctcctcgc cggcctcctc tcgttcatcc cctatgtcgg
      181 ctcgatcacc ggcttcgtcg cctcgatggg cttggccgcc gtccagttcg gcccgaccaa
      241 cttcctgatg ctcggcctca ccgccggcat cttcttcgtc ggccaggccg tcgagggtaa
      301 tttcctgacg cccaagctgg tcggcgaccg catcgggctg cacccggtct gggtgatctt
      361 cgcgctcctg gccggcggtg ccctcttcgg cttcctcggc gtcatgctgg ccgtgccggt
      421 cgccgccgcc atcggcgtgc tggtgcgctt cggcctgcgg caatacctgg cgagccccct
      481 ctacaagggg cacccgctgg acaaagccga ttagctgatg gccctgcgcc gcgcgcgcat
      541 cagctcgacc agggccgcat cgcgctcggc ctcgagctcg gcgacgctgc ggccccgggc
      601 ctcctcggcg acaccggcga cgaggcgctc gcgggcctcg gcatcgaggc gcggctggcc
      661 cagggtcgcc cagcggcgtt cctgactcgg gcccagatgc gcgagatagt cggcgatgcc
      721 cccggccccg ccgcccagat ggtaggagag atgcgccccc tgaaaggccc agcgcagacc
      781 cggcccgtcg accagcgccg tgtcgatatc ggcgacgctg gccaccccct cgcgcaccag
      841 atggaccgcc tcgcgccata gggcagcggc cagccgcccg gcgatatggc ccggcatctc
      901 ccgccgcagc cggaccgggc gcttgccgag ggcggtgtag aaggccgtgg cgtggtcggc
      961 cagcgccggg tcgggcgtga agagctcgac cagcgggatc agatgaaccg gattgaaggg
     1021 atgcgccacg agcaggcggt cgggccgcgc catctcctcc gccagatccg accagaggag
     1081 ggccgaggtg ctgctggcga tcaccgtcgc cggctcggcg gcggcctcga tcctggcgaa
     1141 gagcgcccgc ttgatggcga tatcctcggg cgcgttctcc tgcacgagac ggacaccggc
     1201 caccgcttcc tcgagcgatt tcgccagcac cagccggcca cggcggaccg gctgcagctt
     1261 cttcagcgcc gcccgcagcg gccgcacccg cgcccgcagg ccggcgagac gcgaaggctc
     1321 ggggtcccag gcccgaacct cgagcccggc ggcggtgaag accgcagccc agccggcgcc
     1381 gatcagcccc gagccgacga tggcaaccgg ctcgcggtcg atcggcgcgg tgctcacgcg
     1441 gcccgggccc tgatcttcgc ggcggcgcga tactgctcgg tcgagcgcgg cagcagcgtg
     1501 ccgtcgttcc agtcgcagat gatgccgtgc cggcggatcg cctcgagcat gccgatctca
     1561 cccttccgca gccggccggc cacggcctcg gccggctcct cgagccagcc gccctgccct
     1621 tggcgcagct cgtcgcgcag cttcgccgtc gccgcctcgt cgatccgata gtcgtcgagc
     1681 tcggggtcga cggcctcgac gaccacgccg tagtcgcgcg ccgccccctc gatcgacaca
     1741 tagccgtcga tgacgtcgtc gagcacgcgc gcggtgtcgc gcttcagggg atcgaagagc
     1801 ccgccgccgc cggccgaagg ccggtcgaag cggtcacccg gctgcaaggg gacgttggag
     1861 aagacggctc cgaggaagcg ggattcgttg ccccgcgtgt gggtgacccc gtgcggcgcc
     1921 gagggctggc cgcccatcat gccccaggtg accgatcgcg cccggtcgca gcaataggac
     1981 atgaccgtgc tcttgatcgc cgtgatcgtg ccgcccttgt tgaggccgca gccgccccgg
     2041 aactcaccgg gcccggcgga atcggcggcg atctcgtagc ggtccatgag gatgggggtc
     2101 agccgctcct gggcctcgac cgactgctgg gcgaggccga cgccgaaaat cgaggaggtg
     2161 gcacccgagc cgtccttgcc gttcctcgcc ccccagccac cggccatcca gtcgtaccac
     2221 atgaagaaat tgccgcgccg ctcgggccgg tcgtcgagcc cgccgatcaa gaggtattcg
     2281 aggttgaacg agcaggcgag cgcccgctcc ggcatgaccc tggaccagag ctcgaagatc
     2341 gcgttcatga tcttctcgta ggccccggag cagaaccctg tgacggcata gggatagggc
     2401 gcgttgacga ccgagcccag gggcaggtcg gcgtcgatga agcggtagaa gcccgagttc
     2461 agcggcacct cggggaagaa ggtcttggtg cccgcgacca cggcggagaa ggccgagctg
     2521 gcgctgccgt tcaggaagca gtcaatggcg tcgtgcgagc ccgcgaaatc gtagctgacc
     2581 tggctcccct cgatggtcat cttcacctgg atcgggatca ggccctcggg cttggtcggg
     2641 tcctgatcga tgaagtcggt ggtcgtccag gtgccgtccg gcaggcccgc caccttgtcc
     2701 cgcgcgaacc gctcgacata gtcctggctc tcgcggaacg cctcgagcac cgtatccttg
     2761 ccgtacttct cgaccagctc cagcagccgc tgctcgccga gcctggtggc ctcgcactgt
     2821 gccaggaagt cgcccatggc gtcggcgctg gcgcgggtgt tgccgacgat catctcgacc
     2881 acgtcctcga ggagctggcc tttggtccag acccgggtcg gcgtgatcct gagcccctcg
     2941 ccgaaatggg tgcgggcatt gatgtcgaac gagcccggcg tcgaaccgcc gatatcggcc
     3001 caatggccat tcgactgggt gaaggcgatg agctcgccct cgacgaagac cgggcggacg
     3061 atgcgcacat cgcagaaatg cgtgccgccc gtgtaggggt cgttggagat gaagacgtcg
     3121 cccgggtgga tgtcgccctt gaaccggtcg atcaccgatt tgcaggtgaa atgcagcgtg
     3181 ccgacatgca cggcgatgtc gcccgagccc tgcatcaccg tgttgccctc ggcgtcgcac
     3241 aggcagttgg agaagtcgcg gcagtagatg acgaaggagt agcaggtgcg gagcagctgc
     3301 tccgacatct ggtcgacgag gttgccgtag gcgttcttca gaacctcgaa ggtgacgcgg
     3361 tcgagtgcca tcgagttctg ctcctcaggt gaaacgcatg acgatgtgga gatccggggt
     3421 cacctcgctc ctggtgccgg gcggcaggac cacggtcgaa tcgagctgct cgatgaccat
     3481 cggcccctcg agcaccatgc cggcctcgag cacgtcgcgg cgcaggaccg gggtctcgac
     3541 gaagccgtgc tccttgccga aatgcacggg ccggcgggcc gccgcctcgg gcacgctgcc
     3601 gccggcctgg ctcgccggcg ccggcctcgg cttcggcacg acgccccggg ccacgacccg
     3661 cagcgagtag atctcgacgg tgcggccccg gtcctcgaag gagtaggcgc gggcatgctc
     3721 ctggtggaag cggtcgagca cgtcgtcgag gctcctgagc ttcgccgact cgacgtcgat
     3781 cgtgagctgc cgccactggc cgacatagcg catctcgaag gcgcgctgca ggtgcatgtc
     3841 gcccggcgcc acgccctcct cgcgcagccg gtccttggcc tcggcctcga gcttgtggaa
     3901 ctccgcctcg acctcgtcca gcgtctcgct gctggcaccc ttgaggaaca tggtcgcgaa
     3961 gtcgtggcgg atatcgacca ggagacagcc catggccgag gcgatgcccg gaaatcgcgg
     4021 gaagatgacg accgggatgt cgaggtcgcg ggcgagatcg gcaccatgca gcggtcccgc
     4081 cccgccgaag acgaccagcg cgaaatcgcg cgggtcgaag cctcggccga ccgagatgag
     4141 cttcaccgcg ttcgccatgt tggcgttggc gacgcgcacc accgcatcgg ccgcctcggt
     4201 ctggtcgatg ccgagccggt cgccgacggt gccggtcacg gccgccttcg cggcctcccc
     4261 atcgagcgcc accttgccgt cgagcaggct cgtgccgagc cggccgagca gcacgttggc
     4321 gtcggtgttg gtgggctccg tgccgccggt gccgtagcag gccggccccg gatcggcgcc
     4381 ggcgctctgc gggccgttgc ggagcgagcc gcccgggtcg atccaggcga tggagccgcc
     4441 gccggcgccg atggtgatca cctcgatcga ggggaacatg atcggatagc cgtactcgac
     4501 gtgccagcgc tgggtggtcg gcagcacccc cttgtagcag agcgagatgt ccgtgctggt
     4561 gccgcccatg tccaggctga tggcgttctc gaaaccgcat tgcttcgcga tatgcgcgcc
     4621 ggcgatggcc ccggcgacga tgcccgagct ggcgagccgg gcggcgaacc ggctggcgcc
     4681 ggcggcggtc agcacgccac cacccgaatg caggatgagc aggtcgccgg catagcccgc
     4741 actcttcagc gacctgtcga gggcctcgat gtagcggccg acgaccgggc cggtgacagc
     4801 gttgatcatc gccgtcgagt ggcggggatg ctcgaacatc tcgggcacca cctcggccga
     4861 ggcgcagacg aagacgtccg ggccgagcgc ctcctccagg atcgccttgc agcgctgctc
     4921 gggctcgggg ttggcatagg agttcatgaa gcagacggcg accgcccggt agccgcgctt
     4981 cttcaggagc cgggcgagat cacggacctc ggcctcgtcc aggggcttca ccaccttgcc
     5041 cttgtagtcg acgcgctcgg tcacctcgaa gcggtcgcgg cggcggatat agggcggggc
     5101 cacgtcgaca taggcgtccc agagatcggg cttggtgccg tccctgatct cgacgatgtc
     5161 gcgaaagccg cgggtggcga cgatggcggc cttgggcagc ttgcgggtga tcagcgcgtt
     5221 ggtcgcaacc gtcgtgccgt gggtcaggag cgcgatctcg gccggcgtga tccccgccgc
     5281 ctcgatgctc gccatgaccg cggcgtccgg ctggtccggc ctggacggca ccttggtcac
     5341 ggagatcgca cccgtcgcct cgtcgaagaa ataggcgtcg gtgaacgtgc cgcccgtgtc
     5401 gatggcgacg cggtagcggc ggctgccgcc ctcggcttgc tgggtcatcg tttcgatctc
     5461 tttcagtctc gggtctcagg cggcatcgac ataaggctgg acagccgcag ccaccgcggc
     5521 ggcgacggcc accgcgttcg gcgtgtccga atggacgcag atcgaatcgg cgcggaccgg
     5581 cacgtccttg ccgttgacgc tcgccgtctt gccctcgcga atggcgcgca cgcagcgctc
     5641 ggcggccctt gccggatcga ccgcgtggtg ctcgcgggtg atgatcatca gcccctcgtc
     5701 gctgtagtcg agatcggcat agtactcggc gacgaagcgg tggccgcgtg ccgggtagat
     5761 ggtctcgtgc agcgagttga tgatgccgta gaccggcacc ttgtagacgt ccgccgcgtc
     5821 gcagacggcg tgggcgatgt cctcctgacg tgccgccatg ccgtagagcg agccgtgcgg
     5881 cttgatgtgg ttcagctcga ggccctcggc gtcgaggaag cccttgagcg ccccgatctg
     5941 gtagatcagg cagttggcca gctcctcgcg gccgatcttc atctcccgcc ggccgaagcc
     6001 ctggcggtcc ggcagggacg gatgagcgcc gaccttcacg ccgtgctgct tggcgaggcg
     6061 gacggtgttg cgcatgtggt tgaaatcgga ggcatgaaag ccgcaggcga cattggcgac
     6121 gtggatatgc ggcatcagcc cggcatcgtc gccgatctgg tagagcccgt agccctcgcc
     6181 catgtcgcag ttcacgacgg tcattctctt ctcctcccga agggtgcgcc ggctcagcgg
     6241 gcgacggcgc gaaagcggtc caggaacacg gcgaggatga tcacgctgcc cagcacgatc
     6301 atctgcacat tgccgtcgat gcgggtcaaa ttcatcccgt tggagagcac agtgacgaag
     6361 agggcgccca ggatcaccga gggcacgccg ccgatgccgc cccggagcga gaccccaccg
     6421 atcaccgcgg cggcgatgct ctcgagcgtg aggttgccgc ccagattggg ctcgcccgag
     6481 ccggtccggc cggtcagcat cagggcaccg acggcggcga tcagggagca gagcacatag
     6541 gccagcgtca ggtagcggcg gctcggcagg ccggccacgg ccgcggccgt ggggttgccg
     6601 ccgatcagaa agagcgcccg accgaagacg gtgtggttga ggacgaaatg gaggacggcg
     6661 agcagcagca ccgccagcac caccggcatc ggcacgccca tgaccgagcc gttgtagacg
     6721 agggcggaga actcgcgcgg cacattgaag accggtcttc cgtccgaggc catggtggcg
     6781 atgccgaggc agatattgag gctgccgagc gtggcgacga aggggttgac ccgcagccag
     6841 gcgacggcaa agccgttgaa gaggccgatc agggcaccca tgccgaggcc gaagagcatg
     6901 ccggccgcga tggcgagacc cgtagcctcg ccgccggcgg cgagcgagcc ggtcatggcg
     6961 agcgcgctga cgacactgac cgccgaaacg caggtgccga gcgagaggtc gaaaccgcgg
     7021 gttatgatga ccatcatctg cgccgaggcg aagatcgcga gatagctcgc ctggaccagg
     7081 aggttcagca ggttgcccgc ggaaagcacg cgcgactcga ccagggcgaa gccgagcagc
     7141 agcaggatca gggcgatcgg cagcagcgcc tggacgagga gcgccgtcca ggcgacgcca
     7201 gcgggacggg cagcaccggt gcttttggcc tcagccatgg cggcgccttc ggcgggcata
     7261 ttcctcgagt gccaccgcga ggacgaggat gaagcccagg accaccacct gcatcttcga
     7321 atcgacccgc agcaggttca tggcgttggt caggatcgag aggaagaggg cgccgagcgc
     7381 cacgagctcc acccggccga tgccgcccct gagcgacacg ccggcgatca ccgcggcggc
     7441 gatcgactgc aacatcatgt cgtcaccgcc catggaggcc tggccggagc cgagcctggc
     7501 ggtcatcagc accccggccg tggcggccag gaccgagcac agcacatagg ccaggatgac
     7561 attgagcgtg gccggcacgc cggagacgat ggcggcctgg agattgccgc cgaccgcgta
     7621 gaggtagcgg ccgagacggg tccgggtctg catgaaccag acgagggcga tgatcgcgag
     7681 cgcgatgtag accgtggtcg gcaggccgag ccagagtgca cggccgaact ccttcacgaa
     7741 gacatcgggc atgccgtaga cgggaatacc ctgcgtcagg agcaaagcga gccccgtcgc
     7801 gatcgagagc gtgccgagcg tcaccatgaa gggcgagacg cgcaggaaag cgacgcagag
     7861 cccgttgatc agcccgatga agacgccgca gccgagcccg gcgagcacgc cgagggtgat
     7921 gattgcggcg gtctggtcgg gcatggcggc cgagagggcc gccatcgact tcgccatcac
     7981 cgtgctggtc agcgcgatca ccgcaccgac cgagaggtcg aacccgccga cgatgaggac
     8041 cagcatctgg ccggccgcga cgatcgcgag aaacgaggtc gagcgcagca cgttgaagag
     8101 gttgttgagg ccgaagaact tctgctcgaa gacggccatg aaggcgacgc tgagcaccag
     8161 caggatcggc aggagaccaa ccgaaaggag gcgttgcgag agctgctcgg caccggtcgg
     8221 gggatgggca tggacggcga gcgggcgcac ggtcatgcgg cctgccccgt cttctcgtcc
     8281 tggaagaagc aggcgagcac acgttgctcg gcgatgtcgg cgccggtgag ctccgccacc
     8341 atgcgtcccc gatgcatgac ataaaggcga ttggcgaggt gcagcacctc gggcagctcc
     8401 gaggagacga ggacgatggc ggcacccgaa tcgacgagct gcttcatcag ctcgtagacc
     8461 tcgaccttgg cgccgacgtc gatgccgacc gtcggctcgt cgaagaggaa gacgcgggtc
     8521 gtgcgagtca ggccgcgggc cagcatcacc ttctgccggt tgccgcccga gaggttgccg
     8581 accactcgct cgatgttggg tggccgaagg ccgagctggt cgacgatgcc ctggacgatg
     8641 cgccgctcgc tgccccgctg catgatccgc agccgggcga agcgggtgag gtcgagcgcc
     8701 gccatcgaga cgttctcgcg gatcggccgg ccgagggcca ggccctcgac gacccggtcg
     8761 gaagggaagt agcatacccc gcgctcgagg ctccgggccg gcgagggatg gccataggcc
     8821 tggccctcga cccggatggt gccgccgatg atcgtctcga gcccgtagac cgcgcggatc
     8881 agctccgact tgccgcagcc gacgagaccg gcaattccgg tgatctcacc ggcgcgggcg
     8941 tggaaatcca caccctcgac gctgtggtcg gcgagcgtca ggtcctcgac ctgcagcagc
     9001 gtctcggctg gccggtgatc gacatgcggg aagagcaggt cgatcttgcg gccggtcatc
     9061 agctcgacca gctcgttctc gctgaccccc gcggcatcga gcgtcgcgat cagccggccg
     9121 tcgcgcagca cggtgatgcg gtcgccgagc tgcttgatct cgctcatgcg gtgcgagacg
     9181 tagatgatgc cgatccccga cgccttgagc tgggcgatca gctcgaagag ccggacggtc
     9241 tcgctctcgg tgagcgacgc cgtcggctcg tctaggatga gcacgcgcac gttacccatc
     9301 aacgccttgg cgatctcggt catctgctgg tgggcacggg agagctccac caccttgcgg
     9361 ccgggctcga gggcgaagcc gagatcggcg atggtcc
//
LOCUS       DAOTFI010000193         9311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8830_length_9311_cov_7.117761, whole genome shotgun sequence.
ACCESSION   DAOTFI010000193 DAOTFI010000000
VERSION     DAOTFI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8830_length_9311_cov_7.117761"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1276
                     /locus_tag="P5558_17585"
     CDS             155..1276
                     /locus_tag="P5558_17585"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26186.1"
                     /translation="MSRRIADLRVVLIKAPLEAPIIAPFGRVDVRHNLVVRLKLEGGA
                     EGLGEVWANFPPWGCPERVEILRRVARPALVGETLDDPARLHQMLRARMRGLANQMGA
                     IGPFDQALAGVDIALWDAHSRALGRPLRQVLAEGAADSVAVYATNLPIDRPDMIDAMA
                     AKGHTRFKVKLPADTEMGRRWLPEARAAAGERMLMMDASQGQTPAKLAGIMDALGAAG
                     LAWLEEPFPVDDTEAYREWHGMAGRPPLAMGENSYGESGFEQLLAEIDPDWAQPDITK
                     TAGITRGGRLVAMGKDAGKQVALHMYGGPVGLYASAQLAAALGTVDLVEMDSKPNPLF
                     VNLDRTPEVVEGRLRLPEGPGLGIALDPVVLERFDVTES"
     gene            1281..2261
                     /locus_tag="P5558_17590"
     CDS             1281..2261
                     /locus_tag="P5558_17590"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRY26187.1"
                     /translation="MKIAVFGAGSVGGYLAARLAKAGREVAVIARGDHLAAIRANGLT
                     LETPDERFTVEVEASDDPARIGPVDLVLVTAKTTANQAVAAGIAPLLGPETPVVFAQN
                     GVFWWYGQGFRPPVPASTTRLDPAGRLASAIAPKRRLGLVVHSPNEVRAPGVVRNGST
                     KNRFVLGAADPARPLDDAAAALDGAGFVLERTADIRASMWRKLLVNVSTGPVTALTRA
                     TAAGVVEDRATAAVSRRLIEEALAVAEAHGFLDLGIDPAALSEPGSRPHHKPSMLQDV
                     ERGRALEIDTMARIVQDFAREAEVATPTLDTVLALLVRLARTLGIYAGEG"
     gene            2403..3770
                     /locus_tag="P5558_17595"
     CDS             2403..3770
                     /locus_tag="P5558_17595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY26188.1"
                     /translation="MTMLQGAFTGLLATALLASTTLAPAPAAAEDITLQLWARADPSG
                     PLRAGNIVAATAQLNRMLEAAGSEDRVVIEVFENNAAGFDADALDLFKAFAVDKGPDI
                     FVAAHEWIGAFVEEGLVYQLDEHIAANPELYGDVISSLWNAVAYKGHTYGVPQDSEVR
                     MFFYDKEMLRKIGKDEAFIEGLPDKVESGEFTIYDMTDLAKEVVDAGAAKYGIVHRPN
                     VGPDFQMLMKSFGIDQYDAEAGKLQMSESKLTAFFEWLKYSVDAGVIPANMTAWSWDE
                     VHASFRTDKTSFIKFHGIWNVPPQLEARGLTKDTYFDAIGWTHSPAAEQGGEPANLSH
                     PIIYAVSAESEHPELAAYIVGLASQNMLNTEHAVTTGHTPINYGQAAMPRFIEDGWAL
                     RAGAPMLEYASFMPNHASIGQYNALLFKGIEAVETGRLDPADAAAFVVEELQAELGDE
                     VVILD"
     gene            3846..4721
                     /locus_tag="P5558_17600"
     CDS             3846..4721
                     /locus_tag="P5558_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY26189.1"
                     /translation="MALFLGPAIVLLFMFFIAPVVVDIFVAFTDMGRSLRITELTTAN
                     FERMLTGDSRIGPTLALTFVYVLLTLGIFNVTFGLILALTTTALPERQGSFFRAVWLL
                     PRMSPSVVYALLWAWVIGPTDRGLINQVWVGFLGREPVDMLSNQPMLLIVLANGFIGA
                     SMGMIIFTSAIRAIPEHLFHAARADGAGPLAIVWHVTLPAIRWPLSFITVYQALSLLV
                     SFEYIWLITDGGPFYDTTVYALYVYKRAFENGQYAYGAALALVLVVIGIAASLLMWRF
                     LDMKRLLQTPRIEVQ"
     gene            4718..5818
                     /locus_tag="P5558_17605"
     CDS             4718..5818
                     /locus_tag="P5558_17605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY26190.1"
                     /translation="MSTLAEPARTRAQQPVQQADWDALAARSRRQRRLGQGLLLAFLI
                     TVSVPILLPYFWMFTISVSAKTGGVSSVVLWRACAVLVPTVLAVGLLNALTDRGSTRL
                     YGTAAILAVAGAALAWSVGNELHLQNWKFLHNPNFVQELVGRRASTVESQFPSVWTAF
                     GNSLALALVQTALVLGVGTLAGYYLSRFNFRGRASFLQALLVLHAFPALTLIIPIFLL
                     MYWVGLLDTLTGVILVITTLELPFAVFIMKGFFDAVPWDIEMSALVDGASRRQAFFRV
                     VLPQVKVGLLAVGVFAFIRGWEEYVFVRTLLFAKQNWTMSLYIFWVSDDIMGVDYGVV
                     AAVGVFYVVPSILLYVFCQKYLVQMSLGGIKG"
     gene            5944..7014
                     /locus_tag="P5558_17610"
     CDS             5944..7014
                     /locus_tag="P5558_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26191.1"
                     /translation="MARIELAAVTKAWGDVVAVEPTDLVIEDGEFVAILGPSGCGKST
                     TLFMLAGLYAPTGGAIRFDDAVVNEVEARDRNVGIVFQSYALYPHMSVKKNIRFPLTF
                     KPVAADEVERRVMEAARLVQVEELLERMPAEMSGGQQQRVALARALVKEPQLLLLDEP
                     LSNLDATLRLSMRGEIRRLQRRLGVTTVLVTHDQLEATTMADRIICMSKGRIEQIGTA
                     DDLYHRPDSLFVAGFIGSPPINLLDARGERGRLRVGEAELEATGVAGAVVIGIRPENI
                     GLDPEGGGMAGRIEHVEPLGRETLYEVACPLGRVHALESGSERRFRADDRVDLTLAPD
                     TLLVFDAASGRRLVDASVGLAA"
     gene            7011..7844
                     /locus_tag="P5558_17615"
     CDS             7011..7844
                     /locus_tag="P5558_17615"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRY26192.1"
                     /translation="MTGTALSTEAGSIRLKWHRLRDAADRASHDRASLALGLERGALL
                     EVDLQLTDDSHWVCLHDATLEGETTGKGPVAGRSRFEIEQLRQRHQDGRVLAEAPLFL
                     DELAERLAAAATGARIQLDLKLDRRTLDGPARARFRAAVAPIAGRFDLGGEDRALVQE
                     LAALAPGIRPGFETWPLVGEGRLADRGAAERFARAMVEAAPEAAILYLHYKLIPAAAA
                     FGVDLVGIAHAHGHEVDAWTIDPGIAGLDGLLHHLAEVGCDQITTNAPLALEAAWQRR
                     S"
     gene            7826..8851
                     /locus_tag="P5558_17620"
     CDS             7826..8851
                     /locus_tag="P5558_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY26193.1"
                     /translation="MATTLVTRTTPRLVGPSADWREAALVAPGDAHGRLFDACEALLA
                     VESAGDSAGEAPSGKLLALSWNAERLKYLAASAERLRPLAADVILLTELDRGMARSGN
                     RDTVGELAAALGMAHCFAVEFIELELGDSRERAWHAGIANREGLHGNAILSRWPIEAA
                     GMVRLERDGFWFDGSRNGERRIGGRNAALARVAGITFCSVHLESHSDPEHRALQTTRL
                     LDAVLDFAGADPIVIGGDFNTATVGHGQKGGLDAERLCNPVPFEPLFEIMAERGFAWE
                     AANEMGVATQRTRPDGTPAPPFGRIDWLFVRGLDARDAATLAAVDAAGAAISDHEILS
                     VTVERRR"
     gene            8848..>9311
                     /locus_tag="P5558_17625"
     CDS             8848..>9311
                     /locus_tag="P5558_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY26194.1"
                     /translation="MKLVTYNIQYGLGRDGRYDLARIAREVEAADVIALQEVERFWQR
                     SGVVDEPAEIARHLPRHHWVFGANLDIDADLVEADGRVVHRRRQFGTMILSRRRILSS
                     RNHLLPKYGTLTQHSIQQGTLEAVIDLPMGPLRVYSVHLSHLDAATRLPQIEA"
BASE COUNT         1334 a         3271 c         3253 g         1453 t
ORIGIN      
        1 ggtcgcggtc gattgggggg aacgcacgtc gaagtggaag atgagcaagg gaggtgcttc
       61 cccaactcgc gacgcaccaa cccgagcggg gtgccgcgcg ggggcggggc gtgctaacgc
      121 tcgggcgtgt cctggccgag ggggaggaat gctgatgagc cggcgtatcg cggacctgcg
      181 ggtcgtcctg atcaaggcgc cgctggaggc gccgatcatc gctccgttcg gccgggtgga
      241 cgtgcgccac aacctcgtcg tccggctgaa gctcgagggc ggggccgagg ggctgggcga
      301 ggtctgggcc aatttcccgc cctggggctg ccccgagcgg gtcgagatcc tgcgccgcgt
      361 ggcgcgcccg gcgctggtcg gcgagacgtt ggacgatccg gcccggctgc accagatgct
      421 gcgggcacgg atgcggggcc tcgccaacca gatgggggcc atcgggccgt tcgaccaggc
      481 acttgcgggt gtcgatatcg cgctctggga cgcgcacagc cgggcgctgg gccggccctt
      541 gcggcaggtt ctggccgaag gggcggccga ctcggtcgcc gtctatgcca ccaacctgcc
      601 gatcgaccgg ccggacatga tcgacgccat ggccgccaag ggccacaccc gcttcaaggt
      661 gaagctgccg gccgatacgg agatgggccg tcgctggctg ccggaggcgc gcgccgccgc
      721 cggcgagcgc atgctgatga tggatgcgag ccagggccag accccggcga agctcgccgg
      781 catcatggat gcgctcgggg cggcggggct ggcctggctc gaggagccct ttccggtgga
      841 cgacacggaa gcctatcgcg aatggcacgg catggccggc cggccgcctc tggcgatggg
      901 cgagaacagc tatggcgaga gcggcttcga gcagctcctg gcggagatcg acccggactg
      961 ggcgcagccg gacatcacca agacggcggg catcacccgg ggcggcaggc tggtcgccat
     1021 gggcaaggac gccggcaagc aggtggcgct gcacatgtat ggcgggccgg tcgggctcta
     1081 tgccagcgcc cagctcgcgg cggcgctcgg cacggtcgac ctcgtcgaga tggacagcaa
     1141 gcccaacccg ctcttcgtca atctcgaccg gacgcccgag gtggtggagg ggcggctcag
     1201 gctgccggag gggccgggcc tcggcatcgc cctcgacccg gtcgtgctcg agcggttcga
     1261 cgtgacggag agctgacgcg atgaagatcg cggtctttgg cgccggctcg gtcggcggct
     1321 accttgcggc ccggctcgcc aaagccgggc gcgaggtcgc ggtgatcgcc cggggcgacc
     1381 atttggcggc catccgggcg aacgggctga cgctggagac gccggacgag cgcttcacgg
     1441 tcgaggtcga ggcgagcgac gacccggcgc ggatcggccc tgtcgatctc gtcctggtga
     1501 cggcgaagac cacggcgaac caggcggtcg ccgccggcat cgcgccgctg ctcggccccg
     1561 agactcccgt cgtcttcgcc cagaacgggg tcttctggtg gtacggccag ggcttccgcc
     1621 cgccggtgcc ggcgagcacc acgcggctcg acccggcggg ccggctggca tcggccatcg
     1681 ccccaaagcg tcggctcggg ctcgtcgtgc attcgccgaa cgaggtcagg gcaccgggcg
     1741 tggtcaggaa cggctcgacg aagaaccgct tcgtgctggg tgccgccgac ccggcccggc
     1801 cgctcgacga cgcggcggcg gcgctggacg gcgccggctt cgtgctggag cggacggcgg
     1861 acatccgggc atccatgtgg aggaagctgc tcgtcaacgt ctcgaccggg ccggtcacgg
     1921 cgctgacccg ggcgacggcg gcgggcgtgg tcgaggaccg ggccacggcc gcggtgtcgc
     1981 gccggctgat cgaggaggcg ctggccgtgg cggaggcgca cgggtttctg gatctcggca
     2041 tcgatcccgc ggcgctctcc gagcccggtt cgcggcccca ccacaagcct tccatgctgc
     2101 aggatgtcga gcgcggccgg gcgctcgaga tcgacaccat ggcgcgcatc gtccaggact
     2161 tcgcgaggga ggcggaggtg gcgacgccga cgctcgatac cgtcctcgcc ctgctcgtcc
     2221 gcctcgcccg cacgctcggc atctacgccg gcgagggttg atcccgctgc cgcgattgcg
     2281 tggccgcggc ggaactgtca tgattctgtc gaggcaggcg ggcattggct gaccgcactg
     2341 ccccggcacc ggcggcgcct gcatgacgcc ccgggcacga caaggatggg aggaccacca
     2401 gcatgaccat gcttcagggc gcgttcacgg gccttctggc cacggcactg ctcgcttcga
     2461 cgaccctcgc cccggcgccg gccgcggccg aggacatcac gcttcagctc tgggcccgcg
     2521 ccgatccctc cggccccctg cgcgccggca acatcgttgc cgccaccgcc cagctcaacc
     2581 gcatgctcga ggcggccggc agcgaggacc gcgtggtgat cgaggtcttc gagaacaatg
     2641 ccgccggctt cgatgccgac gcgctcgacc tcttcaaggc gttcgccgtc gacaagggcc
     2701 cggacatctt cgtcgccgcg cacgagtgga tcggcgcctt cgtcgaggag ggtctcgtct
     2761 accagctcga cgagcacatc gccgccaacc ccgagctcta cggcgacgtc atctcgagcc
     2821 tgtggaacgc ggtcgcctac aagggccaca cctacggcgt cccgcaggat tccgaggtcc
     2881 ggatgttctt ctacgacaag gagatgctgc gcaagatcgg caaggacgag gccttcatcg
     2941 aagggctgcc cgacaaggtc gagagcggcg agttcaccat ctacgacatg accgacctcg
     3001 ccaaggaggt ggtcgatgcc ggggccgcca agtacggcat cgtgcaccgg ccgaatgtcg
     3061 gcccggattt ccagatgctc atgaagagct tcggcatcga ccagtacgac gccgaggccg
     3121 gcaagctgca gatgtcggag agcaagctca ccgccttctt cgaatggctg aaatacagcg
     3181 tcgatgccgg cgtcatcccg gccaacatga ccgcctggtc gtgggacgag gtgcatgcga
     3241 gcttccgcac cgacaagacc tcgttcatca agttccacgg catctggaac gtgccgcccc
     3301 agctcgaggc gcgcggcctg acgaaggaca cctatttcga cgccatcggc tggacgcaca
     3361 gcccggccgc ggagcagggc ggcgagccgg ccaatctctc gcacccgatc atctacgcgg
     3421 tctccgccga gagcgagcac ccggagctcg cggcctacat cgtcggcctc gccagccaga
     3481 acatgctcaa caccgagcac gcggtgacca ccggccacac gccgatcaac tacggccagg
     3541 cggcgatgcc gcgcttcatc gaggacggct gggcgctcag ggccggggcg ccgatgctgg
     3601 agtacgcctc gttcatgccg aaccatgcca gcatcgggca gtacaacgcg ctgctgttca
     3661 agggcatcga ggcggtcgag acgggccggc tcgaccctgc cgacgctgcc gcgttcgtcg
     3721 tcgaggagct ccaggccgag ctcggcgacg aagtcgtgat cctcgactga gccgaacggg
     3781 gccggctcca gccggccccg cttcgttcca tgcggcagaa cgcgaacgcg cgccagctcg
     3841 cgaacatggc tcttttcctg ggccctgcga tcgtgctgct cttcatgttc ttcatcgcgc
     3901 ccgtggtggt cgacatcttc gtcgccttca ccgacatggg ccgctcgctc cggatcaccg
     3961 agctcaccac ggccaacttc gagcggatgc tgaccggcga cagccggatc gggccgaccc
     4021 tggcgctcac cttcgtctac gtcctgctga cgctcggcat cttcaacgtc accttcgggc
     4081 tgatcctggc gctgaccacg actgccctgc ccgagcgcca gggcagcttc ttccgcgccg
     4141 tctggctctt gccccggatg agcccgtccg tggtctacgc gctgctctgg gcctgggtca
     4201 tcggcccgac cgatcgcggc ctgatcaacc aggtctgggt cggctttctc ggtcgcgagc
     4261 cggtcgacat gctctccaac cagccgatgc tgctgatcgt gctcgccaac ggcttcatcg
     4321 gtgcgtccat ggggatgatc atcttcacca gcgccatccg cgccatcccg gagcacctct
     4381 tccacgcggc gcgggccgac ggggcggggc cgctcgccat cgtctggcat gtcaccctgc
     4441 cggcgatccg ctggcccctg agcttcatca ccgtctacca ggcgctctcg ctcctggtca
     4501 gcttcgagta catctggctg atcaccgatg gcgggccgtt ctacgacacc accgtctacg
     4561 cgctctacgt ctacaagcgc gccttcgaga acggccagta tgcctatggc gcggcgctgg
     4621 ccctggtgct ggtcgtcatc ggcatcgccg cctcgctcct gatgtggcgc tttctcgaca
     4681 tgaagcggct cctgcagacg ccccggatag aggtccagtg agcacgctcg cggagcccgc
     4741 ccggacgcgg gcgcagcagc cggtgcagca ggccgactgg gatgccctgg ccgcgcggtc
     4801 gcggcgccag cggcggctcg gccagggact gctcctcgcc ttcctgatca ccgtctcggt
     4861 gccgatcctc ttgccctatt tctggatgtt cacgatctcg gtctcggcca aaacgggcgg
     4921 cgtctcgtcg gtggtgctct ggcgcgcctg cgcggtgctg gtgccgaccg tgctggccgt
     4981 gggcttgctg aatgcgctca ccgaccgtgg cagcacgcgg ctctacggca ccgccgccat
     5041 tctcgccgtg gccggggcgg cgctcgcctg gagcgtcggc aacgagctgc atctgcagaa
     5101 ctggaagttc ctccacaacc cgaacttcgt gcaggagctg gtggggcggc gcgccagcac
     5161 ggtggaatcg cagtttccct cggtctggac cgccttcggc aactcgctgg ccctggcgct
     5221 ggtgcagacg gcgctggtgc tcggtgtcgg cacgctcgcc ggctattacc tctcgcgctt
     5281 caatttccgc ggccgggcga gcttcctgca ggcgctgctc gtgctgcacg cctttccggc
     5341 gctcaccctg atcatcccga tcttcctgct catgtactgg gtcgggctgc tcgacacgct
     5401 gaccggggtg atcctggtga tcaccacgct ggagctgccg ttcgcggtct tcatcatgaa
     5461 gggcttcttc gacgccgtgc cctgggacat cgagatgtcg gccctggtcg acggcgcctc
     5521 gcgccggcag gcgttcttcc gtgtcgtgct gccccaggtc aaggtcgggc tgctcgccgt
     5581 cggcgtcttc gccttcatcc gcggctggga ggagtacgtc ttcgtccgca cgctcctctt
     5641 cgccaagcag aactggacca tgagcctcta catcttctgg gtcagcgacg acatcatggg
     5701 cgtcgactac ggcgtcgtcg ccgccgtcgg cgtcttctac gtcgtgccgt cgatcctgct
     5761 ctacgtgttc tgccagaaat acctggtgca gatgagcctc ggcggaatca aggggtagag
     5821 cgccttcatg acgaccgcat acttcgtcaa ccttcggtcg atcggtgctc gcgtcgttca
     5881 cgacgctccg cgccgatcgc ctcggcattc ctcgtcttcg gtcaacctga aggtgctcca
     5941 ctaatggcca gaatcgagct cgccgccgtc accaaggcct ggggcgacgt ggtcgccgtg
     6001 gagcccaccg acctcgtcat cgaggacggc gagttcgtcg ccattctcgg gccctcgggc
     6061 tgcggcaagt cgacgacgct cttcatgctc gccgggctct acgcgcccac cggcggggcc
     6121 atccgcttcg acgacgcggt ggtcaacgag gtcgaggcgc gcgaccgcaa tgtcggcatc
     6181 gtcttccagt cctatgccct ctatccccac atgagcgtga agaagaacat ccgctttccc
     6241 ctgaccttca agccggtcgc ggccgacgag gtcgagcggc gggtgatgga ggccgcccgg
     6301 ctggtgcagg tcgaggagct gctggagcgg atgccggccg agatgtcggg cgggcagcag
     6361 cagcgcgtcg ccctcgccag agcgctggtc aaggagccgc agctcctcct gctggacgag
     6421 cccctctcca atctcgacgc gacgctccgg ctctcgatgc gcggcgagat ccgccggctg
     6481 cagcggcgcc tcggcgtcac caccgtcctc gtcacccacg accagctcga agccaccacc
     6541 atggcggatc ggatcatctg catgtcgaag ggccggatcg agcagatcgg cacggccgac
     6601 gacctctacc atcgcccgga cagcctcttc gtcgccggct tcatcggctc gccgccgatc
     6661 aacctcttgg acgccagggg cgagcgcggc cggctccggg tcggcgaggc cgagctcgag
     6721 gcgaccggcg tcgcgggtgc cgtggtgatc ggcattcgcc cggagaacat cggcctcgac
     6781 ccggaggggg gcggcatggc cgggcggatc gagcacgtcg agcctttggg tcgcgagacg
     6841 ctctacgagg tggcatgccc gctcggccgg gtgcacgcgc tcgagagcgg cagcgagcgg
     6901 cgctttcggg cagacgaccg ggtcgacctg accctggccc ccgacacgct cctggtcttc
     6961 gacgcggcga gcgggcggcg ccttgtcgat gcctcggtcg ggctcgccgc gtgaccggca
     7021 cggcgctctc aaccgaggcc ggctccatcc ggctgaaatg gcaccggctg cgggatgccg
     7081 ccgaccgggc ctcgcacgat cgcgccagcc tggcgctggg cctggagcgg ggtgccttgc
     7141 tcgaggtcga cctgcagctc accgacgaca gccactgggt ctgcctgcac gacgcgaccc
     7201 tcgagggcga gacgaccggc aagggccccg tggccggccg gtcccgcttc gagatcgagc
     7261 agcttcggca gcgccaccag gacggccggg tgctggcgga ggcgccgctc tttctcgacg
     7321 agctggcgga gcggctggct gccgcggcca ctggcgcgcg catccagctc gacctcaagc
     7381 tggaccggcg gaccctggac gggccggcca gggcgcggtt cagggcggcc gtggcgccga
     7441 tcgccgggcg attcgacctg ggcggcgagg accgggcgct ggtgcaggag ctggcggccc
     7501 tcgcacccgg catccggccg ggcttcgaga cctggccgct cgtcggggag ggtcgcctcg
     7561 ccgatcgtgg tgctgccgag cgcttcgcca gggcgatggt cgaggcggcg ccggaggcgg
     7621 ccattctcta cctgcactac aagctgatcc cggcggcggc ggcgttcggc gtcgatctcg
     7681 tcggcatcgc ccacgcccac ggtcacgagg tcgatgcctg gaccatcgat ccgggcattg
     7741 ccgggctgga cgggctgctg caccatctgg ccgaggtcgg ctgcgaccag atcacgacca
     7801 atgcgcccct ggcgctggag gcggcttggc aacgacgctc gtgacgcgga cgaccccccg
     7861 gctcgtcggg ccttcggccg actggcgcga ggcggcgctg gtggcgcccg gcgatgcgca
     7921 cggccggctg ttcgacgcgt gcgaggcgct cttggcagtc gagagtgccg gcgacagcgc
     7981 gggcgaggcg ccatcgggga agctgcttgc cctgagctgg aacgccgagc ggctgaaata
     8041 tctggcggcc agcgccgagc gcctgcggcc gctcgcggcc gacgtcatcc tgctcaccga
     8101 gctcgatcgc ggtatggcgc ggtcgggcaa tcgggacacg gtgggcgagc tcgccgccgc
     8161 gctcggcatg gcgcactgct tcgccgtcga gttcatcgag ctcgagctcg gcgacagccg
     8221 cgagcgggcc tggcatgccg gcatcgccaa ccgggagggg ctgcacggca acgccatcct
     8281 gagccgctgg cccatcgagg cggccggcat ggtgcggctc gagcgcgacg gcttctggtt
     8341 cgacggcagc cgcaacggcg agcggcggat cggcgggcgc aacgccgcgc tggcgcgggt
     8401 cgccggcatc accttctgct cggtccacct cgagagccac agcgaccccg agcaccgcgc
     8461 cctgcagacc acgcggctgc tcgatgcggt cctggacttc gcgggcgccg atccgatcgt
     8521 catcggcggc gacttcaaca cggcgacggt gggccacggg cagaaaggcg ggctggacgc
     8581 ggaacgcctg tgcaacccgg tgccgttcga gccgctgttc gagatcatgg cggagcgcgg
     8641 cttcgcctgg gaggcggcca acgagatggg cgtggcgacg caaaggaccc ggcccgacgg
     8701 cacgccggcc ccgcccttcg gccggatcga ctggctcttc gtccgcgggc tcgacgcgcg
     8761 cgacgccgcg accctggcgg cggtcgatgc cgccggtgcg gcgatctccg atcacgagat
     8821 cctttccgtc acggtggagc gccgccgatg aagctcgtca cctacaacat ccagtacggc
     8881 ctcggccgcg acgggcgcta cgatctcgcc cgcatcgccc gggaggtcga ggccgccgac
     8941 gtgattgcgc tgcaggaggt cgagcggttc tggcagcgct cgggcgtggt cgacgagccg
     9001 gccgagatcg cccgccatct gccccggcat cactgggtct tcggcgccaa tctcgatatc
     9061 gacgcggatc tggtcgaggc ggacgggcgg gtggtgcatc gccgccggca gttcggcacg
     9121 atgatcctca gccggcgccg catcctctcc tcgcgcaacc acctcctgcc gaaatacggc
     9181 acgctgaccc agcacagcat ccagcagggc acgctggagg cggtgatcga cctgcccatg
     9241 ggcccccttc gggtctattc cgtgcatctg agccacctcg atgcggcgac ccgcctgccg
     9301 cagatcgagg c
//
LOCUS       DAOTFI010000194         9289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8851_length_9289_cov_7.080247, whole genome shotgun sequence.
ACCESSION   DAOTFI010000194 DAOTFI010000000
VERSION     DAOTFI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8851_length_9289_cov_7.080247"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..847)
                     /locus_tag="P5558_17630"
     CDS             complement(131..847)
                     /locus_tag="P5558_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26195.1"
                     /translation="MSVDAPMMEGRVVLVTGAGSGLGRAVALALAGAGAQVIATGRRR
                     AALEELDDAVQAASGRSATLLPLDLAETEKLLPIGPSIFERFGRLDGLVHCAAILGKL
                     MPTAQLDTAGLRKALEVNVIATQALIATLTPLLVAAPAGRALFLTDRLASRGRPYLAT
                     YAATKQAMEALVLAFAAEVRQTPLAVRLVAPPAMRSGLRAEAFPGESKAVQTLPETIA
                     GRILELLRADSLPEGDVLDL"
     gene            complement(837..2303)
                     /gene="purF"
                     /locus_tag="P5558_17635"
     CDS             complement(837..2303)
                     /gene="purF"
                     /locus_tag="P5558_17635"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRY26196.1"
                     /translation="MTAAGRSDADEDADRWREECGVYAIYGHPDAAAHAILGLHALQH
                     RGQEAAGIVSFDGAHFNTYRATGLVGDSFSDPRVVEGLPGHAAIGHNRYATTGGSVLR
                     NVQPFFGDFAFGGLAIAHNGNLTNALGLRRRLVEAGCLFQSTSDTEVLVHLIARSGQR
                     SIVERLIDALHQVEGAYSLVGVYDDGIIGARDPLGVRPLVLGRLGDALILASETCALD
                     IIGAELVRDVLPGEVILIDKAGLHSLFPFVRTARRFCIFEYVYFARPDSLVEGRGVYD
                     VRKEIGRELAREAPAAADVVVPVPDSGVPAAIGYAQEAGLPFELGIIRNHYVGRTFIE
                     PTDNIRHLGVRLKHNANRSTLVGKSVVLVDDSIVRGTTSTKIVNMVRAAGATAIHMRI
                     ASPPTTNSCYYGVDTPERSKLLAAQHTLEEMARLIGVDSLAFLSIDGLYRAVGEARRE
                     AGQPQYCDACFTGDYPTRLADADDGGRDRQLTFLQHER"
     gene            complement(2300..2962)
                     /locus_tag="P5558_17640"
     CDS             complement(2300..2962)
                     /locus_tag="P5558_17640"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRY26197.1"
                     /translation="MNDLSITLFDIIVAGIVLLSTLLALSRGAVREILGLLSWIGAIV
                     VAVYAFGPVRPMVTEAIGNELLADAATVAIVFFVPFLVLKIVTSLIGRTVSGSFLGPV
                     DKLLGLIFGAVRGALIVCAAYLVGTAIIAEEQQPDWIKSAKLQPPVAEGADWLAQFLP
                     PGILDGSKAAATEAAARAAEEARRVRDGRNGGTSGSGDGETGYGALVREGMDALIRGK
                     VE"
     gene            complement(2959..4344)
                     /gene="radA"
                     /locus_tag="P5558_17645"
     CDS             complement(2959..4344)
                     /gene="radA"
                     /locus_tag="P5558_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRY26198.1"
                     /translation="MAGTRVQFVCQSCGSVQPKWSGRCDGCGAWNTLVEETSEPLAKG
                     APVKRRSRRGLDLVSLEGTGQPVQRLVTGIGELDRAVGGGMVAGSAILIGGDPGIGKS
                     TLTLQAAARLALAGKRVVYVSGEESIEQIRMRADRIGLAKAPVELASGTSVDEILGTL
                     DTGPPVDLLIIDSIQTVYLEALDPAPGTVTQLRASAQALTRFAKRRGAALLLIGHVTK
                     EGQIAGPRVLEHMVDTVLYFEGERGHQFRLLRAVKNRFGPANEIGVFEMRESGLEPVA
                     NPSALFLAGRDADVSGAAVFAGMEGSRPVLVEIQALVAASPLATPRRAVVGWDSGRLA
                     MLLAVLEARCGLVMGNRDVYLNVAGGLRITEPAADLAVAAALVSSLADRAVPRMTVFF
                     GEVGLGGEVRAVANSEARMKEAAKLGFERVIAPRSRRGDSGEGLAIEELSRLDELLAV
                     MNFSLPEAGTP"
     gene            complement(4360..5148)
                     /locus_tag="P5558_17650"
     CDS             complement(4360..5148)
                     /locus_tag="P5558_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26199.1"
                     /translation="MSSPAMKIRIRGLRKSFGSNHVLQGIDLDVPQGQSVVVIGGSGS
                     GKSVLLKCILGLIEADAGSILVDGEEVVGASARRLTTLRTKFGMLFQSAALFDSLPVW
                     ENVAFGLMAAERTERPEARERAIQTLAKVGLTPAVADQFPSELSGGMKKRVGLARAIC
                     TAPEIIFFDEPTTGLDPIMSDVINDLIRTCVSDLGATALSITHDMGSVRKIADRVAML
                     YDGAIIWEGPVASIEDSGNPYVEQFIRGHADGPIDVGQKVPAGG"
     gene            complement(5145..5921)
                     /locus_tag="P5558_17655"
     CDS             complement(5145..5921)
                     /locus_tag="P5558_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26200.1"
                     /translation="MINPLQPIGAALIGITQTAGRISFFAGNAIGNGFLGPFYWRQLW
                     RQMVEIGFYSLPVVGLTAIFTGMVLALQSYTGFSRFEAESAVATIVVLSMVRELGPVL
                     AGLMVAGRVGAAMAAELGTMRVTEQIDALSTLSTDPLRYLVFPRLIAGLVTLPLLVLV
                     ANVIGVMGGYLVGVHKLGFGPIEYLNRTFEFLETQDVVSGLAKAAVFGFIIALMGCYH
                     GYHSRGGAQGVGTATTHAVVSASILILTANYFITEAFFSR"
     gene            complement(5918..7006)
                     /gene="alr"
                     /locus_tag="P5558_17660"
     CDS             complement(5918..7006)
                     /gene="alr"
                     /locus_tag="P5558_17660"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRY26201.1"
                     /translation="MPEAALLIDLDAIAANYARLRAEAGGCRVAAVVKADAYGLGAER
                     VAARLWAAGCADFFVARLAEGLALRRHLPKARIVVLEGPVPCEVADFVAAALVPTLNT
                     PGQVELWGDAGRRLERPLPAALHLDTGMCRLGISATEAAGLDADRLRAIDVALVMSHL
                     ACAEETANPMNPLQAARFSEMSRRLPAAPRSLANSSGIFLGTAYHFDLLRPGAALYGI
                     NPTPGRPNPMQPVVRIRAPVLQVHRLDAPGTVGYGAAHATSSGSRIATVGVGYADGYM
                     RAAGTGGVAVIAGREVPIVGRISMDLMTLDVTALGEHEVETGTPVDLLAGPGGIDRVA
                     EVAGTIGYELLTRLGQRLRRVYLEATGA"
     gene            complement(7014..8519)
                     /locus_tag="P5558_17665"
     CDS             complement(7014..8519)
                     /locus_tag="P5558_17665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRY26202.1"
                     /translation="MQGSSGGGGGEAVALMRTQPHNLEAEQALLGAVLVNNEAFHRVS
                     EFLKAEHFFEPVHQRIFAACAERIGRGQLADARSLFHLFDEDPALEGLDGAKYLGRIA
                     RAAETIINAVSFGQIIHDLAVRRGLIGVGEEMVLRAYDPQTAETASEQIEMAEQELFG
                     LAQEGEVKSGFRSFPTVLSSTIRLVESAYSKVGEVTGVPSRLDSLDRILGGLQPSDLL
                     ILAGRPSMGKTALAVTIAANAATQKAIGIDGERLKHENYTVGVFSLEMSAEQLAMRLL
                     SAEAQIASDELRRGQLRDDREWQRVVAASQALAARPMFIDDTPALSVAALRSRARRLM
                     RMEGLSLLVVDYLQLMRGSSAYAGTNRVQEVSEITQALKAIAKELNIPVLALSQLSRA
                     VEQREDKRPLLSDLRESGSIEQDADVVMFVYREEYYLTRSEPQQREGESLEKFQIRQN
                     EWMDRCNRSLNKADIIIAKQRNGPIGTVQVQFDARFGRFRNLETAEYIEGG"
     gene            complement(8524..>9289)
                     /locus_tag="P5558_17670"
     CDS             complement(8524..>9289)
                     /locus_tag="P5558_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942124.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRY26203.1"
                     /translation="LGLLHLEIIQERLEREFDLDLVITAPSVVYRLHMTTGEVLELHN
                     PSDYPDPTKIKLVEEPWIKATILVPDDYLGKVLELCTEKRGIQLDLTYVGTRAMLVYR
                     LPLNEVVYDFYDRLKSVSRGYASFDYQLDGMGESDLVKLTVLVNADPVDSLALIVHRS
                     MAEKRGRALCEKLKELIPRQMFKIAIQAAIGGKVIARETISAFRKDVTAKCYGGDITR
                     KRKLLEKQKAGKKRMRQIGNVEIPQEAFLAALKMDG"
BASE COUNT         1467 a         3298 c         3155 g         1369 t
ORIGIN      
        1 ggcagccggc gtcatccccc cggcgtctcc ccctgcccgc aaccctcgaa atggcagcat
       61 tcggctgcgg cttccgcaat cgggcccaaa ccgtgtctag cccatcccca gccgccgcac
      121 ctgcccgtac ctacagatcg agcacgtccc cctccggcag ggaatcggcc cggagcagct
      181 cgaggatccg cccggcgatc gtctcgggca gcgtctgcac ggccttgctc tcgccgggga
      241 aagcctcggc gcgcaagccg ctccgcatgg ccggcggtgc aacgagccgg accgccagcg
      301 gggtctgccg gacctccgcg gcgaaggcca ggaccagcgc ctccatcgcc tgcttcgtcg
      361 ccgcgtaggt ggcgagatag ggccggccgc ggctggctag gcgatccgtc aggaagaggg
      421 cgcgaccggc cggggccgcc accaggagcg gtgtcagggt cgcgatcagc gcctgggtcg
      481 cgatcacgtt gacctccagc gccttgcgca ggcccgccgt gtcgagctgc gcggtcggca
      541 tcagcttgcc caggatcgca gcgcaatgga ccagcccgtc gagccgcccg aagcgctcga
      601 agatcgaggg gccgatcggc aaaagcttct ccgtctcggc gagatcgagc ggcagcaggg
      661 tcgccgaccg gccgctcgcc gcctgcacgg catcgtcgag ctcctcgagc gcggcccggc
      721 gccgcccggt ggcgatcacc tgcgcccccg ccccggccag ggccagcgcc accgcgcggc
      781 cgagcccgct cccggcacca gtcacgagca cgacccgccc ctccatcatc ggcgcatcaa
      841 cgctcatgct ggagaaaggt cagctgtcgg tcacgcccgc catcatccgc gtcggcgagg
      901 cgggtgggat agtcgccggt gaagcaggca tcgcaatatt gcggctggcc ggcctcccgc
      961 cgggcctcgc cgaccgcccg gtagaggccg tcgatcgaca ggaacgccag gctgtcgacc
     1021 ccgatcagcc gcgccatctc ctccagcgtg tgctgggcgg cgagcagctt gctgcgctcc
     1081 ggcgtgtcga cgccgtaata gcagctgttc gtcgtcggcg ggctggcgat gcgcatgtga
     1141 atcgcggtgg cgccggccgc gcgcaccatg ttgacgatct tggtcgaggt cgtgccgcgc
     1201 acgatgctgt cgtcgaccag caccaccgac ttgccgacga gggtcgaccg gttggcattg
     1261 tgcttcagcc gcacgccgag gtggcggatg ttgtcggtcg gctcgatgaa ggtgcggccg
     1321 acatagtggt tgcggatgat gccgagctcg aacggcagcc cggcctcctg cgcgtagccg
     1381 atggccgcgg gcacgccgct gtccggcacg ggcaccacca catccgcggc ggccggcgcc
     1441 tcccgggcca gctcccggcc gatctccttg cggacatcgt acacgccacg gccctcgacc
     1501 aggctgtcgg gccgggcgaa gtagacatac tcgaaaatgc agaagcggcg ggccgtgcga
     1561 acgaacggga agagcgaatg caggcccgcc ttgtcgatca ggatcacctc gcccggcagc
     1621 acgtcgcgca ccagctccgc gccgatgatg tcgagcgcac aggtctccga ggcgaggatc
     1681 agcgcgtcgc cgagccgtcc caggaccagc gggcgcacgc cgaggggatc gcgggcgccg
     1741 atgattccgt cgtcgtagac gcccaccagc gaataggcgc cctccacctg gtggagcgca
     1801 tcgatcagcc gctcgacgat gctccgctgg cccgagcgcg cgatcaggtg aacgaggacc
     1861 tcggtgtcgc tggtcgactg gaagaggcag ccggcctcga ccagccgccg ccgcaggccc
     1921 agcgcgttgg tgagattgcc gttgtgggcg atggcgaggc cgccgaaggc gaagtccccg
     1981 aagaacggct ggacgttgcg cagcaccgag ccgccggtcg tggcgtagcg gttgtggccg
     2041 atggcggcat ggccgggcag gccttcgacc acgcgcggat cgctgaagct gtcgccgacg
     2101 aggccggtgg cgcggtacgt gttgaaatgg gcgccgtcga agctgacgat cccggcggcc
     2161 tcttgccccc ggtgctgcag ggcatgcagg ccgagaatgg cgtgcgccgc cgcatcggga
     2221 tggccgtaga tggcgtagac cccgcattcc tcgcgccacc ggtcggcatc ttcgtcggcg
     2281 tcgcttcgac cagcagcggt cattcgacct ttccccggat cagcgcatcc atgccctcgc
     2341 gaacgagggc accatagccg gtttcgccgt cgccactccc ggaggtgccg ccgttgcgcc
     2401 cgtcacgcac ccgccgtgcc tcctccgccg cccgtgccgc cgcctcggtg gcggccgcct
     2461 tcgagccgtc gaggatgccc ggcggcagga actgcgccag ccagtcggcg ccctccgcca
     2521 ccggcggctg cagcttggcg ctcttgatcc agtcgggctg ctgctcctcg gcgatgatgg
     2581 cggtgccgac gagataggca gcacagacga tcagcgcacc gcgcaccgca ccgaagatca
     2641 ggccgagcag cttgtcgacc ggcccgagga agctgcccga gaccgtccgg ccgatcaggc
     2701 tggtgacgat cttcaggacc aggaagggca cgaagaagac gatggcgacg gtcgccgcat
     2761 cggccagaag ctcgttgccg atggcctcgg tgaccatggg cctgaccggc ccgaaggcat
     2821 agaccgcgac gacgatggcg ccgatccagc tcagaaggcc caggatctcg cgcacggcgc
     2881 cccggctcaa cgccagcagg gtcgacagga ggacaatgcc ggcgacgatg atatcgaaga
     2941 gcgtgatgga aagatcgttc atggggtgcc cgcttccggc agggaaaagt tcatgacggc
     3001 cagaagctcg tccagccggg acagctcctc gatcgcgagg ccctcgccgg agtcgccccg
     3061 ccggctgcgc ggtgcgatca cccgctcgaa gccgagcttg gctgcttcct tcatacgcgc
     3121 ctcgctgttg gcaaccgccc gcacctcgcc gccgaggccg acctcgccga agaagacggt
     3181 catccgtggc acggcccgat cggccagcga ggaaacgagg gccgcggcca ccgcgaggtc
     3241 cgccgcgggc tcggtgatcc gcaggccgcc cgcgacgttc agatagacgt cgcggttgcc
     3301 catcacaagg ccgcaccgcg cctcgagcac cgccagcagc atggccagcc ggccggaatc
     3361 ccagccgacc acggcgcggc gcggcgtggc gagcgggctc gcggccacca gcgcctggat
     3421 ctcgaccagc acgggccggc tgccctccat gccggcgaag accgcggccc cgctgacgtc
     3481 ggcgtcgcgc ccggcgagga agagggccga gggattggcc accggctcca gcccgctctc
     3541 gcgcatctcg aagacgccga tctcgttggc cgggccgaag cggttcttga cggcgcgcaa
     3601 gaggcggaac tgatggccgc gctcgccctc gaaatagagc accgtgtcga ccatgtgctc
     3661 gagcacccga gggcccgcga tctgcccctc cttggtgacg tgcccgatca gcaggagggc
     3721 cgcaccccgc cgcttggcga agcgggtcag cgcctgggcg gaggcgcgga gctgcgtgac
     3781 cgtgccgggg gccgggtcca gcgcctcgag atagacggtc tggatcgaat cgatgatcag
     3841 gaggtcgacc ggcgggcccg tgtcgagcgt gcccaggatc tcgtcgaccg acgtgcccga
     3901 cgcgagctcg accggcgcct tcgcgaggcc gatgcgatcc gcgcgcatgc ggatctgctc
     3961 gatcgattcc tcgccgctca catagacgac gcgcttgccg gcgagcgcca gcctggccgc
     4021 cgcctgcagc gtcagggtcg acttgccgat cccgggatcg ccgccgatga ggatggccga
     4081 ccccgccacc atgccgccgc ccaccgcccg gtccagctcg ccgatcccgg tgacgagccg
     4141 ctgcaccggc tggccggtgc cctcgaggct gacgaggtcg agcccgcgcc gcgagcgccg
     4201 cttgaccggc gcacccttgg cgagcggctc cgacgtctcc tcgaccagcg tgttccaggc
     4261 accgcagccg tcacagcgcc cgctccactt gggctggacg ctgccgcagc tctgacagac
     4321 gaactggacc cgggtgccgg ccatcaggca gtcacggggt cacccacctg cggggacctt
     4381 ctgcccgaca tcgatcggcc cgtcggcgtg cccccggatg aactgctcga catagggatt
     4441 gccgctgtcc tcgatcgacg ccaccgggcc ttcccagatg atcgccccgt catagagcat
     4501 ggcgacccgg tccgcgatct tgcgtacgct gcccatgtcg tgcgtgatcg agagggcggt
     4561 ggcgccgaga tccgagacgc aggtgcggat caggtcgttg atcacatcgg acatgatggg
     4621 gtcgaggccg gtcgtcggct cgtcgaagaa gatgatctcc ggcgcggtgc agatggcccg
     4681 cgcgaggccg acccgcttct tcatgccgcc cgagagctcg ctcggaaact ggtcggccac
     4741 ggccggggtc aggccgacct tcgccagggt ctggatggcc cgctcgcgcg cctccggccg
     4801 ctcggtccgc tcggccgcca tgaggccgaa ggcgacgttc tcccagaccg gcaggctgtc
     4861 gaacaaggca gcgctctgga acaacatgcc gaatttcgtc cgcaaggtcg tcaggcgccg
     4921 tgcgctggcg cccaccacct cctcgccatc gaccaggatg gagccggcat ccgcttcgat
     4981 caggccgagg atgcacttca gcagcaccga cttgcccgac cccgagccgc cgatgacgac
     5041 gaccgactgg ccctgcggca cgtcgaggtc gatgccttgc aggacgtggt tcgagccgaa
     5101 gctcttgcgc agcccgcgga tgcggatctt catggcaggg ctgctcatcg gctgaagaac
     5161 gcctcggtga tgaaatagtt cgcggtgagg atcaggatcg aggcgctgac cacggcatgg
     5221 gtcgtcgccg tgcccacacc ctgcgcccct cctcggctgt gatagccgtg atagcagccc
     5281 atcagggcga tgatgaagcc gaagacggcg gctttcgcca gacccgagac gacgtcctga
     5341 gtctccagaa actcgaaggt ccggttcagg tactcgatcg gcccgaagcc gagcttgtgc
     5401 acgccgacca ggtagccgcc catcacgccg atcacgttgg ccaccaggac cagcagcggc
     5461 aatgtgacga ggccggcgat caggcgtgga aagacgagat agcgcagcgg gtcggtggag
     5521 agcgtgctca gggcgtcgat ctgctcggtc acccgcatgg tgccgagctc ggccgccatc
     5581 gccgcaccga cccggcccgc caccatcagc ccggcgagca ccggcccgag ctcgcgcacc
     5641 atcgagagca ccacgatcgt ggcgacggca ctctccgcct cgaaccgcga gaagcccgtg
     5701 tagctctgca gcgccagcac catcccggtg aagatcgccg tgagcccgac caccggcagc
     5761 gagtagaagc cgatctcgac catctggcgc cagagctggc gccagtagaa cggccccaga
     5821 aagccattgc cgatggcgtt tccggcgaaa aagctgatcc gcccggccgt ctgcgtgatg
     5881 ccgatgagcg cggcgccgat cggctgcaag ggattgatca tgcgccggtc gcttcgagat
     5941 agacacggcg caagcgctgc ccgaggcgcg tcagcagctc gtagccgatc gtgccggcca
     6001 cctcggccac cctgtcgatg ccgccggggc cggcgaggag gtcgaccggc gtgccggtct
     6061 cgacctcgtg ctcgcccagc gccgtcacgt cgagtgtcat caaatccatc gatatccgcc
     6121 cgacaatcgg cacttcacgg ccagcgatca cggcgacgcc gccagtaccc gccgcacgca
     6181 tatagccgtc ggcgtagccg acgcccaccg ttgcaatgcg tgaaccgctg ctcgtcgcat
     6241 gtgcggcgcc gtacccgact gtgccgggag cgtcaaggcg atgcacctgg agcaccgggg
     6301 ccctgatccg cacgaccggc tgcatcggat tgggccgccc cggcgtcggg ttgatgccat
     6361 agagggcggc acccggccgg agcagatcga agtggtaggc cgtgcccagg aagatgccgg
     6421 aggagttggc gaggctgcgc ggggccgccg gcagccggcg gctcatctcg ctgaagcgcg
     6481 ccgcctggag gggattcatc gggttggccg tttcctcggc gcaggccagg tggctcatga
     6541 ccagcgccac gtcgatggcg cgcagccgat ccgcatcgag cccggccgct tccgtggcgg
     6601 aaatgccgag gcggcacatg cccgtgtcca ggtgcagggc cgccggcagg ggccgctcca
     6661 ggcggcggcc ggcgtcgccc cagagctcga cctggcccgg cgtgttcagc gtcggcacca
     6721 gcgcggcggc gacgaagtcc gccacctcgc aaggcaccgg cccttcgagc acgacgatcc
     6781 gcgccttcgg caggtgacgc cggagcgcca gcccctcggc gagccgcgcc acgaagaagt
     6841 ccgcgcagcc ggcggcccaa agccgtgcgg cgacacgctc ggcaccgagc ccgtaggcgt
     6901 cggccttgac caccgcggca acccggcagc caccggcctc ggcccgcagc cgcgcgtagt
     6961 tggcggcaat ggcgtccaga tcgatgagca gggcggcttc cggcatcgcc gcttcagccg
     7021 ccttcgatgt attcggcggt ctcgaggttg cggaatcgac cgaacctcgc gtcgaactgc
     7081 acctgcacgg tgccgatcgg cccgttgcgc tgcttggcga tgatgatgtc ggccttgttg
     7141 agcgaccggt tgcagcggtc catccattcg ttctggcgaa tctggaactt ctcgaggctt
     7201 tccccctcgc gctgctgcgg ctcggaccgg gtcaggtagt attcctcgcg gtagacgaac
     7261 atcacgacgt cggcgtcctg ctcgatcgac cccgattcgc gcagatccga gaggagcggc
     7321 cgcttgtcct cgcgctgctc gaccgcgcgg ctgagctgcg acaaggcgag cacggggatg
     7381 ttcagctcct tggcgatcgc cttgagcgcc tgggtgatct ccgaaacctc ctgcacgcga
     7441 ttggtgccgg cataggcgct cgagccgcgc atcagctgca ggtagtcgac gaccagcagc
     7501 gacaggccct ccatgcgcat cagccgccgc gcccggctgc gcagcgccgc cacggagagg
     7561 gccggcgtgt cgtcgatgaa catcggccgc gccgccaggg cctggctggc ggcgacgacg
     7621 cgctgccact cgcgatcgtc gcggagctgg ccgcggcgca gctcgtccga ggcgatctgc
     7681 gcctcggccg aaaggaggcg catggcgagc tgctcggccg acatctcgag gctgaagacg
     7741 ccgaccgtgt agttttcgtg cttgagccgc tcgccatcga tgccgatggc cttctgggtc
     7801 gccgcgttgg cggcgatggt caccgccagc gccgtcttgc ccatgctcgg gcgaccggcg
     7861 aggatgagca ggtccgatgg ctggaggccg cccaggatgc ggtcgaggct gtccagccgc
     7921 gacggcacgc cggtcacctc gccgaccttc gaataggccg attcgaccag ccggatcgtg
     7981 ctgctcagca ccgtcgggaa ggagcggaag ccgctcttga cctcgccttc ctgggcgagg
     8041 ccgaagagct cctgctcggc catctcgatc tgctcggaag ccgtctccgc cgtctgcggg
     8101 tcgtaggccc gcagcaccat ctcctcgccg accccgatca gcccgcgccg gaccgccaga
     8161 tcgtggatga tctggccgaa gctcaccgcg ttgatgatcg tttccgccgc ccgggcgatg
     8221 cgcccgagat atttcgcacc gtccaggccc tcgagcgccg ggtcctcgtc gaagaggtgg
     8281 aagagcgagc gcgcatccgc gagctggccc cgcccgatgc gctcggcgca ggcggcgaag
     8341 atccgctggt ggacgggctc gaagaaatgc tcggccttca ggaactcgct gacccggtga
     8401 aaggcctcgt tgttgacgag aacagcaccc aggagcgcct gctcggcctc caggttgtgc
     8461 ggctgcgtgc gcatcagcgc gaccgcctcg ccgcccccgc cgcccgagct tccctgcaac
     8521 gccctagcca tccatcttga gcgccgccag gaaggcctcc tggggaatct cgacattgcc
     8581 gatctggcgc atccgcttct tgccggcctt ctgcttctcc aggagcttgc gcttgcgggt
     8641 gatgtcgcca ccgtagcact tcgcggtaac atccttgcgg aaagccgaga tcgtctcccg
     8701 cgcgatgacc ttgccgccga tcgccgcctg gatggcgatc ttgaacatct gccgcgggat
     8761 cagctccttc agcttctcgc agagcgcccg gccccgcttc tcggccatcg accgatggac
     8821 gatgagcgcc aggctgtcga ccgggtcggc gttgaccagg accgtcagct tcacgaggtc
     8881 gctctcgccc atgccgtcga gctgatagtc gaagctggca tagccgcggc tcaccgactt
     8941 cagccggtcg tagaagtcgt agaccacctc gttgagcggc aggcggtaga ccagcatggc
     9001 ccgcgtgccg acataggtga ggtcgagctg gatgccgcgc ttctcggtgc agagctccag
     9061 caccttgccg agatagtcgt ccggcacgag gatcgtcgcc ttgatccagg gctcctcgac
     9121 cagcttgatc ttggtcgggt ccggatagtc cgagggattg tgcagctcga ggacctcgcc
     9181 cgtggtcatg tgcagccggt agaccaccga gggggcggtg atgacgaggt cgaggtcgaa
     9241 ctcgcgctcc agccgctcct ggatgatctc gagatgcaat aaaccgagg
//
LOCUS       DAOTFI010000195         9267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8887_length_9267_cov_6.289514, whole genome shotgun sequence.
ACCESSION   DAOTFI010000195 DAOTFI010000000
VERSION     DAOTFI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8887_length_9267_cov_6.289514"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="P5558_17675"
     CDS             complement(<1..676)
                     /locus_tag="P5558_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26204.1"
                     /translation="MKRRSVLGSVAAGGVAAAGLAAPAIAQGVIEWNMVMPWPRGTPG
                     VGVNAERFAARVTEMSGGRLAIKLYAAGELVPPFEGLDAVSSGTADLGHAPPYFWVGK
                     APVLNYFTGIPFGLTATELAAWFQYGGALELWQEIYKEFNVVPFFAGSSGIQAGGWFR
                     EPINSLADLEGLKFRIAGLGGEVMRRLGVNTVILPPGEISPALLSGAVDAVEWIGPWN
                     DRAFGLQ"
     gene            complement(734..2068)
                     /locus_tag="P5558_17680"
     CDS             complement(734..2068)
                     /locus_tag="P5558_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HRY26205.1"
                     /translation="MTAPTLIVAAPQSGSGKTLLTLGLIRALARRGLKVGSFKVGPDY
                     IDPAFHARASGQSCRNLDSWAMRFETLAALLEDAGRDVDIVVGEGVMGLFDGAPDGSG
                     STADIAALFDLPVVLVAEARRINQSIAALLEGFIGFRQDVEVVGIVLNAVAGERHAAT
                     LLKACDERFSTPVLGWLPRHDELVVPSRHLGLVQAAEHPALDELIERLGSLVAERLDL
                     DRLLRLARRPGVAGLGGQGARPIAALGQRIAVAQDTAFAFAYPAVLEGWRRQGAEIVP
                     FSPLADEAPDPAADAIYLPGGYPELYAGRLAANRQFLLGLQAAAYRDVFIYGECGGYM
                     ALGEVLIDAAGERHRLAELLPVVTSFEAPKLRLGYREVTLLGPSPLGRPPLRYRGHEF
                     HYAREVEQRGVPFLRASDASGRQLGEAGCRLGTVAGSFIHLVDRWPHLVVVR"
     gene            2187..3293
                     /locus_tag="P5558_17685"
     CDS             2187..3293
                     /locus_tag="P5558_17685"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HRY26206.1"
                     /translation="MSMPDPGRQPTAKGQKLRRGWTTGACATAAARAAFSALAGLDFP
                     DPVTIRLPGGQEPSFALLRQERGPGWARASVVKDAGDDPDVTDRAEIVVTLRCRGDGL
                     GLRFRAGEGVGTVTRAGLPLPPGEPAINPVPRRMMTETLEALAIATGHPLDLDLEIAI
                     PGGAELAKRTLNPRLGIEGGLSILGTTGIVIPYSCSSWIHSIHRGVDVARAAGLDHIA
                     ACTGSTSERTVQALYDLPDMALIDMGDFAGGTLKYLRRRPIRRVTLAGGFAKLAKLAQ
                     GHLDLHSSRSTVDLADLAAELGALDPATGSAAEGAVSAGEVLALAGAHRPALARRIAE
                     RARLAAGEAAGPAVAIEALVVDRGGAILAHVPGW"
     gene            3300..4064
                     /locus_tag="P5558_17690"
     CDS             3300..4064
                     /locus_tag="P5558_17690"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF014613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HRY26207.1"
                     /translation="MSATLPHLLLLGGTEEAYELAEALVGRAGLRVTTSLAGATTNPR
                     QPAGSHRIGGFGGTDGLAAWLADEKVALVLDASHPFASRITAQARAAAAMAGILYLRL
                     ERPPWRPGPGDDWRFAADLDAGLMTLAPKARRVFAAVGARALNLLAVSPMSFVVRGVD
                     SPTCLPVNVTFVVGRGPFDLVAERQLFEAERIDAVLCRNSGGEGARAKLDAARELGLP
                     VVMIERSEAPAPGAVADVAAALATLDQLLFGRSTWV"
     gene            complement(4031..4792)
                     /gene="cobM"
                     /locus_tag="P5558_17695"
     CDS             complement(4031..4792)
                     /gene="cobM"
                     /locus_tag="P5558_17695"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HRY26208.1"
                     /translation="MRVHFIGAGPGAADLITVRGLDLIRRSPVILYAGSLVPLEVVAE
                     APGDALIVDTAPLDLDAIIALMQAAEARGEDVARIHSGDPSLYGAISEQIRRLDALGI
                     AWTITPGVPAFAAAAAALGQELTLPGIAQSVVVTRTAVRASTMPEGETLEAFAATGAT
                     LAIHLAINNLTDIRRRLEPILGADAPVIVVYRASWPDQQIVRGTLATIRERVKVAGIT
                     RTALILIGRVLDAEGFAESRLYAADHTHVLRPKRS"
     gene            complement(4789..6543)
                     /gene="cobJ"
                     /locus_tag="P5558_17700"
     CDS             complement(4789..6543)
                     /gene="cobJ"
                     /locus_tag="P5558_17700"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: protein motif:HMM:TIGR01466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HRY26209.1"
                     /translation="MKPLVWHLGPRSAELAERIATALGGATIAGPDVHELRRAFVGGR
                     PLVALAATGIVIRILAPLLVDKRAEPPVLVVDEGGLFVVPLLGGHRGANELAGDLAEA
                     LAARAVVTTAGDARFGLALDAPPPGWRLAGPEAAKLFMARILEGATCRLVDPMGIANW
                     LRAGRLPLATDGELILEVTLEHREPDPLRLVYHPPILALGVGCERDAPADELQRLVAG
                     TLSEAGLAPQAVACVVSVALKAAEPAIHALAAGLDVPARFFTRETLAAEAPRLATPSA
                     VVEAEIGIPGVAEAAALAAVGPAGRLVVPKRKSQRCTCAVALAPAVLAPTTIGRARGR
                     LRVLGIGPGDPGTRTAAVEAALGEADLVIGYGLYLDLVADLTRHAEKRTYPLGKERQR
                     CADAIAEAASGRDVALVCSGDPGIYAMATLVMELLERSNDEAAARIEVSVLPGVSALQ
                     VAAARAGAPLGHDFAAISLSDLLTPMAVIEARLEAAAAGDFALALYNPVSRRRRTALA
                     TARRILLAHRSPETPVVIARNLGRNGETVDVTTLGQLEVDRVDMLSIVLVGAQGTRSF
                     RRVSGGSWTYTPRGYDVS"
     gene            complement(6540..7238)
                     /gene="cobI"
                     /locus_tag="P5558_17705"
     CDS             complement(6540..7238)
                     /gene="cobI"
                     /locus_tag="P5558_17705"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HRY26210.1"
                     /translation="MTSGKLIGVGVGPGDPGLLTLKAVAAIEASPVIAYLSAEGEASL
                     ARNIVAAHLRADHTEVPLQAPMRLEPEVIAGFYDQSADTLAAELQAGRDVALLCLGDP
                     LLYGSFAYLLDRLRHRFAFEVVPGIMSFAAASSKLGRVLARRRDSFAILSSLLPEERL
                     EALIAAADQVAILKVGHRLPMLKAILERQGRLADAMLVAWVGWPEERIVPVADWPADE
                     EPPYFALVLAGRPA"
     gene            complement(7249..8490)
                     /gene="cbiE"
                     /locus_tag="P5558_17710"
     CDS             complement(7249..8490)
                     /gene="cbiE"
                     /locus_tag="P5558_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HRY26211.1"
                     /translation="MTGHWLDVVGIGADGAKGLGDELRALIEGAHLVVGGRRHLAMVD
                     GLGAGERVAWSSPMDAAFERLVAQRGRPPAVVLASGDPLWFGISRLLLQRFNVSELRF
                     HPTVSALQLAAARLGWPLADCLCLSLHGRPLASLARHLAPGRRLLVLTADGASPAAIG
                     EFLAGAGHGHATLTVLEHLGGPDEHRVSFRADEAAARRFADLNTLGIELDAGAVAGHG
                     VSQGLPDDAYIHDGQLTKQDMRAITLAHLAPRPGERLWDIGAGAGSIAIEWLLAGRSC
                     PDRPLTAVAVEARPERLAMLIENAHRLGVPELEVIEGKAPDVLPEGPAPHAIFIGGGV
                     AEPSLIDLCLDRLAVGGRLVVNAVTLGGEARLLDLRRRHGGELARIAISHAEGLGRTL
                     GWKAKAPVTQWVFTRPVAGGL"
     gene            complement(8487..9119)
                     /locus_tag="P5558_17715"
     CDS             complement(8487..9119)
                     /locus_tag="P5558_17715"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HRY26212.1"
                     /translation="MHAYLREPESIYARSFALIEAEAGAALAALPAELRPVARRLIHS
                     CGMTDIPTDLAWSGQVVTAARNALECGAALFCDVEMVKAGIIRRRLPAAVEPSCTLAD
                     PEVPALATMLGTTRSAAAVELWRKRLNGAVVVIGNAPTALFHLLERLALWPERPAAIF
                     AFPVGFVGAAESKDALIAASPAVPFLTLRGRRGGSAMAAAAVNALWDPQR"
BASE COUNT         1432 a         3239 c         3375 g         1221 t
ORIGIN      
        1 tctgcaggcc aaaggcgcgg tcgttccagg ggccgatcca ctcgaccgcg tcgacagcac
       61 cggagagcaa agcgggggaa atttcgccgg gcggcaggat gacggtgttg acgccgagcc
      121 gccgcatcac ctcgccgccg aggccggcga tgcggaactt gagcccctcg aggtcggcca
      181 ggctgttgat cggctcgcga aaccagccgc ccgcctggat gccggaggag ccggcgaaga
      241 aggggacgac gttgaattcc ttgtagatct cctgccagag ctcgagggcg ccgccatact
      301 ggaaccaggc agcgagctcg gtggcggtga ggccgaaggg aatgccggtg aagtagttga
      361 gcaccggcgc cttgccgacc cagaaatagg gtggcgcatg gccgagatcc gccgtgccgg
      421 atgagacggc atcgaggccc tcgaagggtg gcaccagctc gcccgccgca tagagcttga
      481 tggccagccg gccgcccgac atctcggtga cccgggcggc gaaacgttcg gcattgacgc
      541 cgacgcccgg cgtgccgcga ggccagggca tgaccatgtt ccactcgatg acgccctggg
      601 cgatggcggg tgcggcaaga ccggcagcgg ccacgccacc cgcggcgacg ctacccagaa
      661 ctgaacggcg tttcatgagc tcgacctccc tgctgacaat cctgcgtctc ataagaccat
      721 ttgtcgtcgt ccgctagcgg acgacgacaa ggtgtggcca gcggtcgacc aagtggatga
      781 aggagccggc gacggtgccg agccggcaac cggcctcgcc cagctgcctg ccgctagcat
      841 cgctggcccg caggaagggc acgccgcgct gctcgacctc gcgcgcgtag tggaactcgt
      901 ggccgcgata gcggagcggc ggccggccga gcggcgaggg gccgagcagg gtcacctcgc
      961 gatagccgag gcggagcttc ggtgcctcga agctggtgac caccggcagg agctcggcca
     1021 ggcggtgacg ctcgccggcc gcgtcgatca gaacctcgcc cagcgccatg tagccgccgc
     1081 attcgccgta gatgaagacg tcgcgatagg ccgcagcctg caggccgagc agaaattgcc
     1141 gattagccgc gagccgcccg gcatagagtt cggggtagcc tccgggcagg tagatcgcgt
     1201 cggcggccgg gtcgggcgcc tcgtcggcca aaggcgagaa cggcacgatc tccgcaccct
     1261 gccggcgcca gccctcgagc accgctgggt aggcgaaggc gaaggcggta tcctgggcca
     1321 ccgcgatccg ctgccccagg gcggcgatcg gccgggcacc ctggccaccc aggcccgcca
     1381 cgccgggccg gcgggcgaga cggagcaggc ggtcgaggtc gagccgctcg gcgaccagcg
     1441 agcccaggcg ctcgatcagc tcgtcgaggg ccgggtgctc ggccgcctgg acgaggccca
     1501 gatgccgcga cggcacgaca agctcgtcgt ggcgcggcag ccagcccagg accggcgtgg
     1561 agaaccgctc atcgcaggct ttcaggaggg ttgcggcatg gcgttcgccg gccaccgcat
     1621 tgagcacgat gccgaccacc tcgacgtcct ggcgaaaccc gatgaagccc tcgagcaggg
     1681 cggcgatcga ctggttgatg cggcgcgcct cggcgaccag cacgaccggc agatcgaaga
     1741 gggcggcgat gtcggcggtc gagccgctgc cgtccggcgc gccgtcgaaa agacccatca
     1801 ccccctcgcc gacgacaatg tcgacgtcgc gcccggcgtc ctcgagcagg gcggccagcg
     1861 tctcgaagcg catcgcccag ctgtcgagat tacggcagct ctgcccggag gcgcgggcat
     1921 ggaacgccgg gtcgatatag tcgggcccga ccttgaagct gccgaccttg agcccgcgcc
     1981 gggccaaagc acggatcagg ccgagcgtca ggagtgtctt gccggagccg gattgcggtg
     2041 cggcgacgat cagggtgggc gccgtcaagg ctggcctccg gcatggccct cgggcacaat
     2101 gcgcgttcag gggcagaggg tcatgcccgg tctgatagcg gaatttgccc gtcgatgccc
     2161 agccgtcacg gtgcttcggt cacaccatga gtatgcccga ccccggccgc cagccaacgg
     2221 ccaaggggca gaagctgcgc cgcggctgga ccaccggcgc ctgcgccacg gccgccgcca
     2281 gggcggcctt ctcggccctg gccggcctcg actttcccga cccggtgacc atccgcctgc
     2341 ccgggggcca ggaaccgagc ttcgcgctgc tgcggcagga gcggggccca ggctgggcgc
     2401 gggcgagcgt ggtcaaggac gcaggcgatg atccggatgt caccgaccgg gcggaaattg
     2461 tcgtgaccct gcgctgtcgc ggcgacggcc tcggcctgcg gtttcgcgcc ggcgagggtg
     2521 tgggcacggt gacccgggcc ggcctgccgc tgccgccggg cgagcccgcg atcaacccgg
     2581 tgccgcggcg gatgatgacc gagacgctgg aagcgctcgc catcgccacc ggccatcctc
     2641 tcgacctcga cctcgagatt gccattccgg gaggcgccga gctggcgaag cgcacgctca
     2701 acccgaggct cggcatcgaa ggcgggctct cgatcctcgg caccaccggc atcgtcatcc
     2761 cctattcctg ctcgtcctgg atccactcga tccatcgggg tgtcgacgtc gcccgcgccg
     2821 ccgggctgga ccacatcgcc gcctgcaccg gcagcacctc ggagcggacc gttcaggcgc
     2881 tctacgacct gccggacatg gcgctgatcg acatgggcga tttcgccggc ggcacgctca
     2941 aatacctgag gcggcggccg atccggcggg tcacgctcgc cggcggcttc gccaagctcg
     3001 ccaagctggc ccaaggccat ctcgacctgc attccagccg ctcgaccgtc gacctcgcgg
     3061 atcttgcggc cgaactcggc gctctcgacc cggccacggg aagtgctgcc gagggcgcgg
     3121 tgtccgccgg cgaggtgctg gcgctcgccg gtgcccaccg cccggcgctg gcccggcgca
     3181 tcgccgagcg ggcgcggctg gcggcgggcg aggcggccgg cccggcggtg gcgatcgaag
     3241 cactcgtcgt ggatcgcggc ggcgccatcc tcgcccatgt ccccggctgg tgaagaacca
     3301 tgtccgccac gctgccgcat ctcctgctcc tcggcggcac ggaagaggcg tatgagctgg
     3361 ccgaagccct ggtcgggcgt gccgggctca gggtcaccac ctcgctcgcc ggcgccacga
     3421 ccaacccgcg ccagccggcc ggtagccatc ggatcggcgg cttcggcggt actgatgggc
     3481 tcgcggcttg gctcgccgac gaaaaggtgg cgctcgtgct cgatgccagc caccccttcg
     3541 ccagccggat caccgcccag gcccgggctg ccgcggccat ggccggcatt ctttatctcc
     3601 gcctggagcg gccgccatgg cgacccggtc cgggcgacga ctggcgcttc gctgccgacc
     3661 tcgatgccgg cctgatgacg ctggcaccga aggcgcggcg ggttttcgcc gcggtcggtg
     3721 cccgagcgct gaacctgctg gctgtgtcac ctatgagctt cgtggtgcgc ggcgtcgatt
     3781 cgccgacgtg cttgccggtc aatgtgacct tcgtcgtcgg ccgcggtccc ttcgacctcg
     3841 tagccgaacg gcagctcttc gaggcggagc ggattgatgc cgtcctctgt cgcaacagcg
     3901 gcggcgaggg ggcccgtgcc aagctggatg ccgcacgcga gctgggcctg ccggtcgtca
     3961 tgatcgaacg atccgaggct cccgcgcctg gtgccgtggc ggacgtggcg gcagcgctcg
     4021 cgaccctgga tcagctcctt ttcggccgca gcacgtgggt gtgatcggcg gcatagaggc
     4081 ggctctcggc gaagccttcg gcatccagca cccggccgat caggatgagc gccgttcgcg
     4141 tgatgccggc aaccttcacc cgctcacgga tggtggcgag cgtgccccgc acgatctgct
     4201 ggtcgggcca gctcgcccga tagacgacga tgaccggggc atcggcaccg aggatgggct
     4261 cgaggcgccg gcgaatgtcg gtcaggttgt tgatcgcgag gtggatggcg agggtggcgc
     4321 cggtggcggc gaaggcttcc agcgtctcgc cctcgggcat ggtcgaggcc cgcaccgccg
     4381 ttcgggtcac gacgacgctc tgggcgatcc cgggcagggt cagctcctgg cccagggctg
     4441 ccgccgccgc ggcgaaagcc ggcacgccgg gggtgatggt ccaggcgatg cccagggcat
     4501 ccagacggcg gatctgctcc gagatggcac catagagcga ggggtcgccc gaatggatgc
     4561 gggccacatc ctcaccccgc gcctcggccg cctgcatgag tgctatgatg gcatcgaggt
     4621 ccagcggggc cgtgtcgacg atcagggcgt cgcctggggc ttccgccacg acctccagcg
     4681 gcacgagcga gccggcatag aggatcaccg gcgaccgacg gatcaggtcc agcccgcgca
     4741 ccgtgatgag gtccgcagca cccggcccgg cgccgatgaa atgaaccctc atgaaacgtc
     4801 gtagcccctc ggcgtgtagg tccaggagcc gcccgagacg cgcctgaagc tgcgggttcc
     4861 ctgagcgccg accaggacaa tggagagcat gtcgacccgg tcgacctcga gctgcccgag
     4921 cgtcgtgacg tcgaccgtct cgccgttgcg gccgagattg cgggcgatca ccaccggcgt
     4981 ctcgggagag cggtgggcca gcaggatgcg gcgggccgtc gccagcgccg tccgtcggcg
     5041 ccgcgatacc gggttgtaga gggcgagggc gaagtcgccg gctgcagcag cttccaagcg
     5101 tgcctcgatg acggccatgg gcgtcaggag atcggagagc gagatggcgg cgaagtcgtg
     5161 gccgagcggt gcgccggcgc gcgcagccgc cacctgcagc gccgagacgc ctggtagcac
     5221 gctcacctcg atacgtgcgg cggcctcgtc gttgctccgc tcgagcagct ccatgaccag
     5281 ggtcgccatg gcgtagatgc ccggatcgcc ggagcagacg agcgccacgt cgcgcccgct
     5341 ggccgcctcg gcgatggcgt cggcacagcg ctggcgttcc ttgccaagcg gataggtgcg
     5401 cttctcggca tgacgggtga ggtcggccac gaggtcgaga tagagcccgt agccgatcac
     5461 gaggtcggcc tcgccgaggg cggcctcgac cgcggccgtt cgggtgccgg gatcgcccgg
     5521 gccgatgccc agcacccgca agcggccgcg cgcccggccg atggtcgtgg gagcgagcac
     5581 ggccggtgcc aatgccaccg cacaggtgca acgctggctt ttgcgttttg ggacgaccag
     5641 ccggccggcg gggccgacgg ccgccagggc cgccgcctcc gccacgcccg gaatgccgat
     5701 ctcggcctcc accacggcgg aaggcgtcgc cagccgcggc gcctcggccg ccagggtctc
     5761 gcgcgtgaag aagcgggccg ggacgtcgag tccggcggcg agggcgtgga tcgcgggctc
     5821 ggccgccttg agcgcgacag agacgacgca ggcgacggcc tgaggagcga ggccagcctc
     5881 actgagcgta ccggcgacca gtcgctgcag ctcgtcggcc ggtgcgtcgc gctcgcagcc
     5941 gacaccgagc gccaggatcg gcgggtgata gacgagccgg agagggtccg gctcccgatg
     6001 ctcgagggtg acctcgagga tgagctcgcc gtccgtagcg agcggcagcc tgccggcccg
     6061 cagccagttc gcgatgccca tgggatccac gaggcggcag gtggctccct ccaggatgcg
     6121 tgccatgaag agtttcgcgg cttcgggccc ggcaagtcgc cagcccggtg gcggcgcatc
     6181 gagggcgagg ccgaagcggg cgtcgcccgc cgtcgtcacg accgcccggg cggccagcgc
     6241 ctccgccaga tcccccgcga gctcgttggc accccgatgg ccgccgagca gcggcacgac
     6301 gaagagccca ccctcgtcga cgaccagcac cggtggctcg gcccgcttgt cgacgaggag
     6361 cggtgccaga atgcggatga cgatgccggt cgcggcgagc gccaccaggg gccgcccgcc
     6421 aacgaaggcg cgccgcagct catggacgtc cggcccggca atcgtggccc cgccgagtgc
     6481 cgtggcgatc cgctccgcca gttcggcgct gcgcggtccc agatgccaga cgagcggctt
     6541 catgccggtc ggccggcgag cacgagtgcg aaatagggtg gctcctcgtc ggccggccag
     6601 tcggcgaccg gcacgatccg ctcctcgggc cagccgaccc aggcgacgag catcgcatcg
     6661 gcgagccgcc cctgccgctc gaggatcgcc ttgagcatgg gcaggcggtg cccgaccttg
     6721 agaatggcca cctgatcggc cgccgcgatc agggcctcga ggcgctcctc ggggagaagg
     6781 gaggagagga tcgcgaagct gtcccggcgc cgcgccagca cccggccgag cttcgaggaa
     6841 gcggcggcga aggacatgat gccgggcacc acctcgaagg cgaagcggtg gcgcagccgg
     6901 tcgagcaggt aggcgaagct gccgtagagc aggggatcgc cgaggcagag cagcgccacg
     6961 tcgcgccccg cctggagctc ggccgccagg gtgtcggcgg actggtcgta gaagcccgcg
     7021 atcacctccg gctccaggcg catcggtgct tggagcggga cttccgtgtg atcggcgcgc
     7081 aggtgcgcgg caacgatgtt ccgcgccagg cttgcttccc cctcggccga gagatacgcg
     7141 atcaccggcg aggcttcgat ggccgccacc gccttcaatg tcaggagacc gggatcgccc
     7201 ggcccgacgc cgacgccaat gagcttgccg ctggtcatcg atcgcccctc agagcccgcc
     7261 ggccactggc ctggtgaaca cccactgggt gaccggcgcc ttggccttcc agccgagcgt
     7321 gcggccgagc ccctcggcgt ggctgatggc gatccgcgcc agctcgccgc catgccggcg
     7381 gcggagatcg agcagccggg cctcgccgcc gagggtcact gcgttgacga ccagccggcc
     7441 gccgacagcc agccggtcaa ggcagagatc gataagggag ggctcggcga ccccgccacc
     7501 gatgaagatc gcatggggtg ccggaccttc cgggagcacg tccggcgcct tgccctcgat
     7561 cacctcgagc tcgggcacgc ccaggcgatg cgcgttctcg atcagcatgg cgaggcgctc
     7621 gggccgcgcc tcgaccgcga cggcggtgag cggccggtcc ggacaggacc ggccggcgag
     7681 cagccattcg atggcgatgg agccggcgcc ggcgccgatg tcccagagcc gctcgcccgg
     7741 tcggggagcc aggtgcgcga gcgtgatggc gcgcatgtcc tgcttggtga gctggccgtc
     7801 atggatatag gcatcgtccg gcaggccctg gctcacgcca tgaccggcga cagcaccggc
     7861 atccagctcg atgcccaggg tgttgagatc ggcgaaccgc cgcgctgccg cctcgtcggc
     7921 gcgaaagctc acccgatgct cgtccggccc ccccagatgt tcgagcacgg tgagcgtggc
     7981 atggccatgg ccggcgcccg ccaggaactc gccgatggca gccggcgaag ccccgtccgc
     8041 ggtcaggacc agcagccggc gaccgggtgc caaatggcgg gcgaggctcg cgagcggccg
     8101 gccgtgcagg ctgaggcaga ggcagtcggc gagcggccag cccagtcgcg cggcggcgag
     8161 ctgcagggcc gagaccgtgg gatgaaagcg cagttcgctg acgttgaagc gctggaggag
     8221 gaggcggctg atgccgaacc acaagggatc gcccgaggcg agcacgacag ccggtgggcg
     8281 cccgcgctgg gcgacaagac gttcgaacgc ggcgtccatc gggctcgacc atgcgacacg
     8341 ctcaccggcg ccgagcccgt ctaccatggc caagtggcgg cggccaccga ccacgagatg
     8401 ggcaccctcg atcagcgcgc gaagttcgtc gccgaggcct ttggcgccat cggcaccgat
     8461 ccccaccacg tcgagccagt ggccggtcat cgctgggggt cccaaagcgc gttgacggcg
     8521 gcggcggcca tcgcactgcc gccgcgccgg ccgcgcaggg tcaggaaggg cacggccggc
     8581 gatgccgcga tgagcgcatc cttcgactcg gctgccccga caaaacccac aggaaaggcg
     8641 aaaatcgccg ccgggcgctc gggccagagg gccagccgct cgagcagatg gaaaagggcg
     8701 gtgggcgcgt tgccgatgac caccacggca ccattgagcc gcttccgcca gagctcgacg
     8761 gcggcagcgc tgcgcgtcgt gcccagcatt gtcgcgagcg ccggaacttc tggatcggcg
     8821 agcgtgcagc tcggctcgac cgcggccggc agccgacgac ggatgatccc ggccttgacc
     8881 atctccacat cgcaaaagag cgccgcaccg cattcgaggg cgttgcgagc agccgtgacg
     8941 acctggccgg accaggcgag gtcggtcggg atgtcggtca tgccgcaact gtggatcagc
     9001 cgccgcgcca ccggccgcag ctccgccggc aatgccgcca acgccgcgcc cgcctcggcc
     9061 tcgatgaggg cgaaggagcg ggcgtaaatc gactcgggct cgcgcagata ggcatgcatg
     9121 gcgatggtca tggcggaggg gcgtggcccg cgcaaggcag tcctgcgacc cggcttgctt
     9181 cgcttatcgc acctgctaag gcgacgccat gtggaccgac agtctcttca tcagtatcgc
     9241 cgccgccctg ctgatcgtgc gcgaggc
//
LOCUS       DAOTFI010000196         9244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_8929_length_9244_cov_6.318968, whole genome shotgun sequence.
ACCESSION   DAOTFI010000196 DAOTFI010000000
VERSION     DAOTFI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8929_length_9244_cov_6.318968"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="P5558_17720"
     CDS             complement(<1..244)
                     /locus_tag="P5558_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRY26213.1"
                     /translation="MSLDDDALRRYARHIVLPEVGGRGQERLLAAAVLVVGMGGLGAP
                     LALYLAAAGIGRLGLVDDDVVASSNLHRQVLYTEADI"
     gene            334..639
                     /locus_tag="P5558_17725"
     CDS             334..639
                     /locus_tag="P5558_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRY26214.1"
                     /translation="MSGANDYAVAVRYLPEAGKLPEVTAKGRGAIAAQILGIAAAYDI
                     PIERDPDLVTILEKLDVGSPIPTVAFAAVAEILAHLYRANAGAQAATGNTLPPAGRP"
     gene            636..926
                     /locus_tag="P5558_17730"
     CDS             636..926
                     /locus_tag="P5558_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26215.1"
                     /translation="MSAATELTTALAAARRSLAGGGLPDIEGLAGRLASLLAEAGRSG
                     RSRDLVALIGLLDEVARLGEALSRECSACRAELARGDASARATAAYHNRARS"
     gene            939..1238
                     /locus_tag="P5558_17735"
     CDS             939..1238
                     /locus_tag="P5558_17735"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRY26216.1"
                     /translation="MLEYADLSRTIIGLGFVLLLMAAVFWLVRRFGPAAARGRFAASR
                     RLAVLETLPLDPRTRLVLLRHDGAEHLVLVSSQGSASLLTTNTAGRPPIDRDDQP"
     gene            1235..1987
                     /gene="fliP"
                     /locus_tag="P5558_17740"
     CDS             1235..1987
                     /gene="fliP"
                     /locus_tag="P5558_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944807.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRY26217.1"
                     /translation="MSGRRLALGLLAGLLPVFLASPAAAQALSIDLGGGETATGQIVR
                     LVLLVTVLSLAPSILLMVTSFTRIVIVLSFLRTALGLQQSPPNSVLVSLAMFLTLFIM
                     MPTFERAWEDGIGPLIAEDIDEMTALERAATPFHAFMRSQVRDKDLALFYDIAAIEPT
                     LDPAEAPFRVLVPAFMISELRRAFEIGFLIFIPFLIIDMVVASILMSMGMMMLPPVVI
                     SLPFKVIFFVLVDGWLLVVGSLVQSFGAIPVP"
     gene            2078..3451
                     /locus_tag="P5558_17745"
     CDS             2078..3451
                     /locus_tag="P5558_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRY26218.1"
                     /translation="MERVIELLQARLGERVTTNRVLREQHGRGEGPYGGAAPDAVVTP
                     ASTEEVAFVVATCRDALVPIVAFGAGTSLEGHVEALRGGICIDLSAMDRILDVSVEDL
                     DCHVEAGIRRRRLNEELRDTGLFFPVDPGADASLGGMAATRASGTNAVRYGTMRDVVL
                     GLTAVMADGSIVHTGGRARKSAAGYDLTRLLIGSEGTLGIITELRLRLFGIPEAISAA
                     VATFPDVRSAVETVQLTIQSGIPVARIELLDAVQMRACIAHSKLAGLEPAPTLFFEFH
                     GSAAGVAEQAAAVEAIAGDFGGTGFRWSASTEERNQLWRARHDAYYAALALRPGCRGW
                     ATDVCVPISKLAACIAETEADLAESRLLAPLVGHVGDGNFHLLFLLDPDDPDEQARAK
                     AVNARLIDRALALGGTCTGEHGIGFGKIASLEREHADGMAVMRAIKTALDPASILNPG
                     KVLRLEP"
     gene            3561..5312
                     /locus_tag="P5558_17750"
     CDS             3561..5312
                     /locus_tag="P5558_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26219.1"
                     /translation="MESFAAGLATCLLLAGAAAAQDLAEVPRERTLVTIGWSAGSPTL
                     SAPNNANWYSLGAELRNGIMFVNEPLFTYNHFTGEHIPWIAESYEYNDDYTSLTVKLR
                     DGVTWSDGEAFDAEDVAFTLNMLLENGKTKKDLRKAIEVANQITGAEVIDPLTVRVDL
                     IKPDPRYIYRQLTNYFGHGLIWVPEHIWSGIEDIANFTFLDLEKGWPVGTGAWKVVET
                     TPNEIVLDRRDDWWGAATGVMEMPAPERIITIPAPDDERNAQLVVANQIDATFVIASP
                     SLVQSILDQNDRITTFAGRNPPYGYMDWWPHSLYFNHMAEGSPFLDINVRRAVLHAIN
                     RDQLIEFAWDGANSPNYFPYPAYEPLLPYIDSAKDLVEKYQVDVFDLDLSAKYMTDAG
                     YEKDGEGFWAKDGERVGGPLEAQPALAAVSQVLVQQLRKAGFDAEYSQTPDSFRKFRA
                     GESQWHVFGHNGGSIFDPVDTLRMYKTENQSPIGTITFFVARWSNPELDAIVDQMELL
                     PPGDPGLMPLFHEAMDIWFANVVEVPLEQGYHLNALNETYWTNWPSTENPYGSACVAC
                     FISGWGTLLAHHVQPVQ"
     gene            5373..6380
                     /locus_tag="P5558_17755"
     CDS             5373..6380
                     /locus_tag="P5558_17755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26220.1"
                     /translation="MIRVTKRLGLMVLVIWLAASINFLLPRLTDRDPIEERLAQALGD
                     SGGSAVGIEEMVEQYKAKFGTDRPLWRQYLSMLGDTARFDLGVSINYFPDRVSQHVLT
                     ALPWSIVLLGVATLISFVIGTFLGALTIWRRSSAWLARIVPLFMVLAAIPFYLVGLIL
                     VYVFALVLGWFPPARGYSVGLNLGWNPTAIADVAYHAVLPALSIVLAAIGIWALSMRG
                     MMVTVQGEDYMNFARAKGLSDRRRFWRYAVRNAMTPQITTLVLSFGNIVAGAILVERV
                     FVYPGIGTLLYNSVLLVDYFLIFGCVFVLILTVAISLMLLDLLYPLLDPRIRAGGEAN
                     G"
     gene            6373..7281
                     /locus_tag="P5558_17760"
     CDS             6373..7281
                     /locus_tag="P5558_17760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26221.1"
                     /translation="MARALPATPLEWGSSRRRWRSVLLYLGRNRSLGAGLLLLLALFT
                     LVAAGHAFYDIALAEPLSASPGKPPSARYWLGTDKQGRDLFAVLIVGTWLTAKVGLLA
                     GFFGVVIGTVLGFLAGFFRGWTDAVISLLTDVFLAVPPLLILIVIAANIKGSMSTDSM
                     VWIIAALAWREPARRIRSQVLVMREAGYVNTARLNGTGAFGIIFKEMMPNLLPYLMVS
                     FVLATATAVLASVGLEALGLGPQNEPTLGMTVYWAIHYSAFLLGMWWWVLTPVITLVV
                     LFVGLYLVTAGLDELANPRLRTGGAR"
     gene            7281..>9244
                     /locus_tag="P5558_17765"
     CDS             7281..>9244
                     /locus_tag="P5558_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26222.1"
                     /translation="MAGLEVRGLKVVFETDDGIVEAVRGIDFDVQPGERLGFVGESGS
                     GKTTTALALMGMIDAPGRVAGGTALLGGVDLLRLSPAEHAARRLVDVAYIPQGAMNSL
                     NPVMRVGAQIADGMIDHGTGLGRAARKARVGELLRQVGLEPSVAERFPHELSGGMKQR
                     VAIAISIALRPQLLIADEPTSALDVITQRRVMQTLREVQDAIGAGLILIGHDMGLMAQ
                     FADRIMVLRHGRLVEEGQVRQIFREPKDAYTKELIDSVPALEQRRASVIAAFGDANAA
                     PIIELDRAAKTYRRGLFDRTGVPALQPLSFSMAGGTPLVVSIVGQSGSGKSTLGSLLL
                     GFLAPSEGRVLFEGRDLQTLSGAERRESHRRVQAVFQDPYAAYNPFYKVDHNLAEPLR
                     NFGLARDRASVLLKMREACELVGLDPDQTIHRFAHQLSGGQRQRLMVGRELAEVDEDL
                     AASHERAARAGKRDPVLRVSGLRDARLLQDISFELAPGEILGLAGLMGSGRSEVAEAV
                     FGISEAEGEVEIAGEAFSERTPQATKARGVALVSEDRRADQVFAGRSVRENLTSAIID
                     TLSGAGSYLRPSTQREEAARMAEAYAVRHPGLEAPIGSLSGGNQQKCIIARWLATKPR
                     ICILDEPTKGIDVGAKADIHRLVAQLAEQGL"
BASE COUNT         1349 a         3396 c         3021 g         1478 t
ORIGIN      
        1 cgatatccgc ctcggtgtag agcacctgcc ggtgcaggtt ggagctcgcg accacgtcgt
       61 cgtcgacgag gccgaggcgg ccgatcccgg cggcggcgag atagagggcg agcggggcgc
      121 cgagcccgcc catgccgacc accagcacgg cagcggcgag cagccgctcc tggccccgcc
      181 cgccgacctc cggcagcacg atgtgccgcg cgtagcggcg aagcgcgtcg tcatcgaggc
      241 tcacgccctg ctctccttcg cggcaaccgc gttaacgccc gcttaaccct gttggcctag
      301 cgtccgcacc gggcaacgag cgcgggacga ggcatgagcg gggcgaacga ctacgcggtg
      361 gcagtgcggt atctacccga ggccggcaag ctgccggagg tgacggccaa ggggcgcggt
      421 gccatcgccg cccagatcct cggcatcgcc gccgcctacg acattcccat agagcgcgat
      481 cccgatctgg tcaccatcct cgagaagctc gacgtcggca gcccgatccc gaccgtcgcc
      541 ttcgccgccg tggccgagat cctggcccac ctctaccgcg ccaatgccgg ggcgcaagca
      601 gcgaccggca acaccctgcc gccggcgggc cggccatgag cgccgcgacg gagctcacga
      661 ccgccctcgc agcggcccgt cgctcgctcg caggcggcgg ccttcccgat atcgagggcc
      721 tggccgggcg cctcgcaagc ctcctcgccg aggcgggccg cagcgggcga agccgggatc
      781 tcgtggccct gatcggcctg ctcgacgagg tggcacggct gggcgaggcg ctgtcgcgcg
      841 aatgcagcgc ctgccgcgcc gagctggcgc gcggcgatgc cagcgcccgg gcgacggccg
      901 cctaccacaa ccgtgcccgc tcctgagggg ccggagcgat gctggaatac gccgatctca
      961 gccggaccat catcggccta ggattcgtcc tcctgctgat ggcggccgtc ttctggctgg
     1021 ttcgccgctt cggcccggcg gcggcgaggg gccggttcgc cgcgagccgc cggctcgccg
     1081 tgctcgagac cctgccgctc gacccgcgca cgcgcctcgt gctgctgcgc cacgacgggg
     1141 ccgagcatct ggtgctggtc tcgtcgcagg gctcggccag cctgctcacc acgaacaccg
     1201 ccggccggcc gcccatcgac cgggatgacc agccatgagc ggccggcgcc tggctctcgg
     1261 cctcctcgcc ggccttcttc cggtcttcct ggcgagcccg gccgcggccc aggcgctctc
     1321 catcgatctc ggcggcggcg agaccgccac gggccagatc gtccgcctcg tgctgctggt
     1381 cacggtgctc tcgctggcac cgtcgatcct gctgatggtg acctcattca cccggatcgt
     1441 gatcgtcctc tccttcctgc gcaccgccct cggcctgcag cagagcccgc ccaactcggt
     1501 cctggtcagc ctcgccatgt tcctgaccct cttcatcatg atgccgacct tcgagcgggc
     1561 ctgggaggac gggatcggcc cgctcatcgc cgaggacatc gacgagatga cggcgctcga
     1621 gcgggccgcc acgccgttcc atgccttcat gcgcagccag gtgcgggaca aggatctcgc
     1681 cctcttctac gacatcgcgg ccatcgagcc gacgctcgac ccggccgagg ccccgtttcg
     1741 cgtgctggtg ccggccttca tgatcagcga gctcaggcgg gccttcgaga tcggtttcct
     1801 gatcttcatc cccttcctca tcatcgacat ggtcgtggcc tcgatcctga tgtcgatggg
     1861 catgatgatg ctgccgccgg tggtcatctc cctgcccttc aaggtgatct tcttcgtcct
     1921 cgtcgacggc tggctgctgg tcgtcggcag cctggtccag agcttcggcg ccatacccgt
     1981 tccctgacac ccccctcggc tccggcgcca tcggctgcta cgatgaaccg gacgagcagg
     2041 ggagcggcga gccatggcga ttgcggaacc tcgggacgtg gagcgagtga tcgagctcct
     2101 gcaggcccgg ctcggcgagc gggtgaccac caaccgcgtg ctccgcgagc agcacggccg
     2161 gggcgagggt ccctatggcg gggcggcacc ggacgccgtg gtgacgccgg cctcgaccga
     2221 ggaggtggcc ttcgtcgtcg ccacctgccg ggatgcgctc gtgccgatcg tcgccttcgg
     2281 cgccggcacc tcgctcgagg gccatgtcga ggctctccgg ggcggcatct gcatcgatct
     2341 ctcggccatg gaccgcattc tcgacgtctc ggtcgaggat ctcgactgcc acgtcgaggc
     2401 cggcatccgg cggcgccggc tgaacgagga gctgcgcgac acgggcctct tcttcccggt
     2461 cgaccccggc gcggacgcct cgctcggcgg catggccgcc acccgggcct cgggcacgaa
     2521 tgccgtgcgc tacggcacga tgcgcgacgt ggtgctgggc ctcaccgcgg tcatggccga
     2581 cggcagcatc gtgcataccg gcggccgcgc ccgcaagagt gccgcgggct acgacctgac
     2641 gcgcctgctg atcggctcgg agggcacgct cggcatcatc accgagctgc gcctgcgcct
     2701 gttcggcata cccgaagcca tctcggcggc ggtcgccacc ttcccggacg tgcggtcggc
     2761 ggtcgagacc gtgcagctca ccatccagtc gggcattccg gtggcgcgga tcgagctgct
     2821 cgacgccgtg cagatgcggg cctgcatcgc ccactcgaag ctcgccgggc tggagccggc
     2881 accgaccctc ttcttcgagt tccacggcag tgccgcaggg gtggccgagc aggcggcggc
     2941 ggtcgaggcg atcgccggcg atttcggcgg cacggggttt cgctggtcgg ccagcaccga
     3001 ggagcgcaac cagctctggc gcgcgcgcca cgacgcctat tacgcggccc tggcgctccg
     3061 ccccggctgc cgcggctggg ccaccgatgt ctgcgtgccg atctcgaagc tcgccgcctg
     3121 catcgccgag accgaggccg acctcgccga aagccggctc ctcgccccct tggtcggcca
     3181 tgtcggcgac ggcaatttcc atctcctctt cctgctcgac cccgacgacc cggacgagca
     3241 ggcgcgggcc aaggcggtca atgcccggct gatcgaccgc gccctggcgc tgggcggcac
     3301 ctgcacgggc gagcacggca tcggcttcgg caagatcgcc agcctcgagc gcgagcatgc
     3361 cgacggcatg gcggtgatgc gggccatcaa gacggcgctg gacccggcga gcatcctcaa
     3421 ccccggcaag gtgctgcgcc tcgagccctg acgccgatca cgaaatttga tcaaattcca
     3481 cccgggttgt tgaccttgtg ggcaaggccc ggcatgtttt tgatctgaag acgataacta
     3541 ggggggcttt gatgtcgagg atggagtcgt ttgccgccgg gctcgcgacg tgcctgctgc
     3601 tggccggtgc cgcggctgcg caggacctgg ccgaggtgcc gcgcgagcgc acgctggtca
     3661 ccattggctg gagcgccggc tcgccgacct tgagcgcgcc caacaacgcc aactggtatt
     3721 cgctgggtgc ggagctccgc aacggcatca tgttcgtcaa cgagccgctc ttcacctaca
     3781 accacttcac cggcgagcac attccctgga tcgccgagag ctacgaatac aacgacgact
     3841 acacgagcct gacggtcaag ctgcgcgacg gcgtgacgtg gagcgacggc gaggccttcg
     3901 acgccgagga tgtcgccttc accctcaaca tgctgctcga gaacggcaag acgaagaagg
     3961 acctcaggaa ggcgatcgag gtcgccaacc agatcaccgg tgctgaggtc atcgatccgc
     4021 tcaccgtccg ggtcgacctg atcaagcccg acccgcgcta catctatcgc cagctcacca
     4081 actatttcgg ccacgggctc atctgggtgc ccgagcacat ctggtccggg atcgaggaca
     4141 tcgccaactt caccttcctc gacctcgaga agggctggcc ggtcggcacc ggggcctgga
     4201 aggtggtcga gacgacgccc aacgagatcg tgctcgaccg gcgcgacgac tggtggggtg
     4261 ccgccacggg tgtgatggag atgccggcgc cggagcgaat catcaccatc ccggccccgg
     4321 acgacgagcg caacgcgcag ctcgtcgtcg ccaaccagat cgacgccacc ttcgtcatcg
     4381 cctcgccgtc gctggtgcag tcgatcctcg accagaacga caggatcacc accttcgccg
     4441 ggcgcaaccc gccctacggc tacatggact ggtggccgca ctccctctac ttcaaccaca
     4501 tggccgaggg ctcgcccttc ctcgacatca acgtgcgccg ggcggtgctg cacgccatca
     4561 atcgcgacca gctcatcgag ttcgcctggg acggcgccaa ctcgccgaac tacttcccct
     4621 accccgccta cgagccgctt cttccataca tcgacagtgc gaaggacctg gtcgagaaat
     4681 accaggtcga cgtcttcgat ctcgacctct cggccaagta catgaccgac gccggctacg
     4741 agaaggacgg cgagggcttc tgggccaagg acggcgagcg cgtcggcggg ccgctcgagg
     4801 cgcagccggc cctggcggcg gtctcccagg tgctggtcca gcaattgcgc aaggccggct
     4861 tcgatgccga gtactcgcag acgcccgaca gcttccgcaa gttccgcgcc ggcgaatccc
     4921 agtggcacgt cttcggccac aacggcggca gcatcttcga cccggtcgac acgctgcgca
     4981 tgtacaagac ggagaaccag tcgccgatcg gcacgatcac cttcttcgtc gcccgctgga
     5041 gcaacccgga gctcgacgcc atcgtcgacc agatggagct gctgccgccc ggcgaccccg
     5101 ggctcatgcc gctcttccac gaggccatgg acatctggtt cgccaatgtg gtcgaggtgc
     5161 cgctcgagca gggctaccac ctgaacgccc tgaacgagac ctactggacc aactggccgt
     5221 cgaccgagaa cccctacggc tcggcctgcg tcgcctgctt catctcgggc tggggcacgc
     5281 tgctcgccca ccacgtccag cccgtccagt agccaatcgg agaaccgcgg gcggccttcc
     5341 cggtcgcccg cggcctctcg acgagacaag cgatgatccg cgtcacgaag cgtctcggcc
     5401 tgatggttct cgtcatctgg ctcgcggcgt cgatcaactt cctcctgccg cgcctgaccg
     5461 accgcgaccc gatcgaggag cggctggcgc aggcgctcgg cgattccggc ggctcggccg
     5521 tcggcatcga ggagatggtc gagcagtaca aggccaagtt cggcaccgac cggccgctct
     5581 ggcggcaata cctctccatg ctgggcgaca ccgcccgctt cgatctcggc gtctcgatca
     5641 actacttccc cgaccgggtc tcgcagcacg tgctgaccgc cctgccctgg agcatcgtgc
     5701 tgctcggcgt ggcgacgctg atctcgttcg tgatcggcac ctttctcggt gccttgacga
     5761 tctggcggcg ctcctcggcc tggctggcgc ggatcgtccc gctcttcatg gtgctggccg
     5821 ccatcccgtt ctacctggtc gggctcatcc tcgtttacgt cttcgccctc gtgctgggat
     5881 ggttcccgcc ggcccggggc tacagcgtcg ggctgaacct cggctggaac ccgaccgcca
     5941 tcgccgacgt cgcctaccac gccgtgctgc ctgccctttc catcgtgctg gccgccatcg
     6001 gcatctgggc gctctcgatg cgcggcatga tggtgacggt gcagggcgag gactacatga
     6061 acttcgcccg ggccaagggg ctgagcgacc gccgccgctt ctggcgctac gccgtgcgca
     6121 acgccatgac gccgcagatc acgacgctgg tcctctcctt cggcaacatc gtcgccggcg
     6181 ccatcctggt cgagcgcgtc ttcgtctacc cggggatcgg cacgctgctc tacaactcgg
     6241 tgctgctggt cgactatttc ctcatcttcg gctgcgtctt cgtcctcatc ctgacggtcg
     6301 ccatctcgct gatgctgctc gacctgctct accccctgct cgatccccgg attcgcgccg
     6361 gtggagaggc caatggctag ggccctgccg gcgacaccgc tcgaatgggg ctcatcccgc
     6421 cgccgctggc ggtcggtcct cctctatctc ggccgcaacc gcagcctcgg cgccggcctc
     6481 ctcctgctgc tggcgctctt cacgctggtc gccgccggcc acgccttcta cgacatcgcg
     6541 ctcgccgagc cgctctcggc ctcacccggc aagccgccct cggcgcgcta ctggctcggc
     6601 accgacaagc agggccgcga cctcttcgcc gtcctcatcg tcggcacctg gctgaccgcc
     6661 aaggtcggcc tgctcgcggg cttcttcggc gtcgtgatcg gcaccgtcct gggcttcctc
     6721 gccggcttct tccgcggctg gaccgatgcc gtgatcagcc tgctcacgga cgtcttcctc
     6781 gccgtgccgc ccttgctgat cctgatcgtc atcgccgcca acatcaaagg ctcgatgagc
     6841 acggacagca tggtctggat catcgcagcg ctcgcctggc gcgagccggc gcggcggatc
     6901 aggagccagg tcctggtgat gcgcgaggcg ggctacgtca acacggcccg gctcaacggc
     6961 accggcgcct tcggcatcat cttcaaggag atgatgccca acctcctgcc ctatctgatg
     7021 gtcagcttcg tgctcgccac cgccaccgcc gtgctcgcct cggtcgggct cgaggccctc
     7081 ggcctcgggc cgcagaacga gccgacgctc ggcatgaccg tctactgggc catccactac
     7141 tcggccttcc tgctcggcat gtggtggtgg gtgctgaccc cggtgatcac gctcgtcgtc
     7201 ctcttcgtcg ggctctacct cgtcaccgcc gggctcgacg agctggccaa tccccgcctc
     7261 cgtaccgggg gtgcgcgctg atggccggcc tggaggtgcg ggggcttaag gtcgtcttcg
     7321 agacggatga cggcatcgtc gaggccgtgc gcggcatcga cttcgatgtg cagccgggcg
     7381 agcggctcgg cttcgtcggc gaatccggct cgggcaagac gaccacggcg ctggcgctca
     7441 tgggcatgat cgatgcgccc gggcgcgtcg ccggcggcac ggcactgctc ggcggcgtcg
     7501 atctcttgcg cctctcgccg gcggagcacg cggcccggcg gctggtcgac gtcgcctaca
     7561 tcccgcaagg ggcgatgaac tcgctcaacc ccgtcatgcg cgtcggtgcc cagatcgccg
     7621 acggcatgat cgaccacggc accggcctcg gccgtgcggc gcgcaaggcg cgggtcggcg
     7681 agctcctgcg ccaggtcggg ctcgagccgt cggtcgccga gcggttcccg cacgagctct
     7741 cgggcggcat gaagcagcgg gtggcgatcg ccatcagcat agcgctccgg ccgcagctcc
     7801 tgatcgccga cgagccgacc agcgctctcg acgtcatcac ccagcgccgc gtcatgcaga
     7861 cgctgcgcga ggtccaggac gccatcggcg ccggtctgat cctgatcggc cacgacatgg
     7921 ggctgatggc gcagttcgcc gatcgcatca tggtgctccg gcacgggcgc ctagtcgagg
     7981 aaggccaggt ccgccagatc ttccgcgaac cgaaggacgc ctacacgaag gagctgatcg
     8041 acagcgtgcc ggcgctcgag cagcgccggg cgagcgtgat agcagcgttc ggggacgcca
     8101 acgccgcccc cattatcgag ctcgaccggg cggccaagac ctaccgccgc ggactcttcg
     8161 accgaacggg cgttccggcg ctccaaccct tgagcttctc catggccggc ggcacgccgc
     8221 tcgtcgtctc gatcgtcggc cagagcggca gcggcaagtc gaccctcggc agcctgctcc
     8281 tggggttcct cgccccgagc gagggccgcg tcctcttcga gggccgcgac ctccagaccc
     8341 tttcgggggc ggagcggcgg gagagccacc gccgcgtgca ggccgtcttc caggacccgt
     8401 acgccgccta caacccgttc tacaaggtcg accacaacct cgccgagccc ctgcgcaatt
     8461 tcggcctcgc ccgggaccgg gcgtcggtgc tcctgaagat gcgcgaggcc tgcgagctgg
     8521 tcggcctcga ccccgaccag acgatccacc gcttcgccca ccagctcagc ggcggccagc
     8581 gccagcggct gatggtcggc cgggagctgg cggaggtcga cgaggacctc gccgcaagcc
     8641 acgagcgcgc cgccagggca ggcaaacgcg accccgttct ccgcgtcagc ggcctcaggg
     8701 atgcccggct cctccaggac atcagctttg aactggcgcc gggcgagatt ctcgggctcg
     8761 cgggcctgat gggttcgggg cgctccgagg tggccgaggc ggtcttcggc atttcggagg
     8821 ccgaaggcga ggtcgagatc gccggcgaag cgttcagcga gcgcaccccg caggccacca
     8881 aggcacgcgg cgtcgcgctg gtgtcggaag accgccgggc cgaccaggtc ttcgccgggc
     8941 gcagcgttcg cgagaacctc acctcggcga tcatcgacac gctttccggc gccgggtcct
     9001 atttgcgccc gtcgacccag cgcgaagagg ccgccaggat ggcggaggcc tacgcagtcc
     9061 ggcaccccgg cctcgaggcg ccgatcggct cgctgtcggg cggcaaccag cagaagtgca
     9121 tcatcgcccg ctggctcgcg acgaagccga ggatctgcat cctcgacgag cccaccaagg
     9181 gcatcgacgt gggcgccaag gcggatatcc atcgcctcgt cgcgcaactg gcggagcagg
     9241 ggct
//
LOCUS       DAOTFI010000197         9123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9085_length_9123_cov_6.766211, whole genome shotgun sequence.
ACCESSION   DAOTFI010000197 DAOTFI010000000
VERSION     DAOTFI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9085_length_9123_cov_6.766211"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="P5558_17770"
     CDS             <1..1139
                     /locus_tag="P5558_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY26223.1"
                     /translation="AIEVAGIGSREDLYWTLHAVFVNRRDQRELFDQCFHIFWRDPQL
                     LERLMHLMLPTLRHDQPGEEEELSRRVAEALTPEKQPGRGEAPEDEKEEEIELDAVLT
                     YSAKELLQEKDFEEMSADELADAKRQIERLRLPIVALPTRRFQGDPHGRRIDLRRTLR
                     QSMRGGGAVIDLARKKRVRRHPPLVILCDISGSMSRYSRMLLLFMHALTGDRDRVHSF
                     VFGTRLTNITRYLRNKDVDIALDRVGEVVKDWAGGTRIGHCLEAFNRDWSRRVLGQGA
                     VVMLITDGLDRDAGDGLEQEMERLHKSCRRLIWLNPLLRYEGFQPISSGARAMVAHVD
                     DFRTVHNLNSLRDLADVLNREPHRRAEGVSVWAAEQEETGAHAG"
     gene            1129..1461
                     /locus_tag="P5558_17775"
     CDS             1129..1461
                     /locus_tag="P5558_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRY26224.1"
                     /translation="MLDDVVLDDTLLDSAAEWHAAGRRVAIATVVSTWGSSPRPPGSQ
                     LLIDDQGNMLGSVSGGCIEGAVVKKALDIIDGAPPEVMEFGVSNEQAWEVGLACGGRV
                     QVYVEALS"
     gene            1471..2190
                     /locus_tag="P5558_17780"
     CDS             1471..2190
                     /locus_tag="P5558_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRY26225.1"
                     /translation="MRPATLELLNEARAAKRHACLVRNLDSHAEALVVDGEVMLGSVS
                     PALLAEVEACLRADRSRPVEVPEGRALLQIFNPPLRLAIIGAVHIAQMLAPMAAIAGY
                     AVTVIDPRGAFATEERFPGVTLSHDWPDEAMEAFAPDRRSAIVALTHDPKLDDPALDE
                     ALRSDAFYIAALGSKRNHAARMGRLRELGHTDQAIARIHGPAGLDIGAVSPAEIAISV
                     MAQMTAVLREPRDVASRGEAA"
     gene            2187..3806
                     /locus_tag="P5558_17785"
     CDS             2187..3806
                     /locus_tag="P5558_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HRY26226.1"
                     /translation="MKFGTIPVDEAEGALLAHSIRLDGETLKKGRLLTGDDLDTLRDA
                     GMATVVAARLEPDDVPEDTAAARLAMTLGGPAIRVAEPFTGRANVFAETDGVFTVDKR
                     TIDGVNGLDEAITIATVADHAAVSEGQMLATVKIIPFAAPEAAVNQALAILNDRAPVL
                     RLHPFRPLVARLIQTSLPDTNTKMLDKTARITGERMAAVGGSLASESRCGHTTEALAD
                     QITGALAQGCDLLLIAGASAITDRRDVLPAGIEAVGGRIRHFGMPVDPGNLLLLAALD
                     DVPVLGLPGCARSPKLNGFDWVLQRLAAGLEVDRATIMGMGVGGLLTEIPTRPQPRTR
                     KRSVRDESRIAVLVLAAGQSRRMGPTNKLLIPIDGKPMLRHTVEAALTSKANDVIVVT
                     GHEHAAVEAILAGTAVRIVHNPAFADGLSTSLKAGIAALPPGIDAALVCLGDMPEIDA
                     ELIDRLIAAFEPGSGRSIVVPTHNGKRGNPVLWGSAFFPAMLAIEGDVGARHLIGQHA
                     EAVVEVAFDNASSLVDIDTPEALEAYLTGQD"
     gene            3806..5023
                     /locus_tag="P5558_17790"
     CDS             3806..5023
                     /locus_tag="P5558_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY26227.1"
                     /translation="MGVGRELRPQFPIFASFPEPLHYLDNAATGQICAAAAEALWEFE
                     TAARANVKRGVYRLADQASAAFEAARSTMASYIGAPAPDEVVFTSGATSALNIAAHGL
                     AERLSPGDEILVSVLEHHSNIVPWQLAAKARQAVVKAIPVTDDGRLDLDRLGGVLTDR
                     TRVVSLTHVSNVTGAVTDLARLRAAADDVGAILVVDGAQRAPHGPIDVKAIGADLYAF
                     SAHKMFGATGAGVLWGRSAILDAFPPLLGGGEMIREVSFAGTTFAAPPHRFEAGTPPI
                     GPVLAMAAAAEWLMAQDWSALAAHEARLTGRLLDGLGSLPGVRILGPTGLQQRLGVVA
                     FEVDGVHAHDVCQLVDQLAGVALRGGHHCCQPLMERFDTIATARASLAPYNDDDDIDA
                     LIDGLERTIEMLQ"
     gene            5020..5451
                     /locus_tag="P5558_17795"
     CDS             5020..5451
                     /locus_tag="P5558_17795"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRY26228.1"
                     /translation="MNEALYNRAIIEAARDKRHAGRLEHPGGTATTDNPLCGDRVTLD
                     LEGEAGRIAAVAHKTRGCLLTQAAASLLAAHAVGRPIDEVVPLRGAIRAYLDGTAEDP
                     PFPELAMLAPVRAVKSRHECVTIAFDALAEAAVQLATEKPS"
     gene            5448..5849
                     /gene="msrB"
                     /locus_tag="P5558_17800"
     CDS             5448..5849
                     /gene="msrB"
                     /locus_tag="P5558_17800"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015088687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRY26229.1"
                     /translation="MNDKVVKDDAEWRASLDPEQYRVAREQGTERPFTGRYWNTKEAG
                     TYACVCCGQPLFASTTKFDSGTGWPSFWAPIDEEAVATRTDHSHGMTRTEALCARCDA
                     HLGHVFPDGPEPTGLRYCLNSASLDLKPQSD"
     gene            6214..9045
                     /locus_tag="P5558_17805"
     CDS             6214..9045
                     /locus_tag="P5558_17805"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY26230.1"
                     /translation="MTPASDTDTGDARAPVVIDLTEVDTAGATFELNEARLILRFADG
                     QEQTLAELPLQEPAHVRLADGGAIELGALAEALTAADGPLPVPAAIAELAEAAPEAGA
                     EPVSGGAEFRLMQIDDLGASPDPTMQLGAVEYGRTLGGDSEFFTRSGALDGSGPLGST
                     GNGGPGGGGLGSGPNGGGFINNPPVARHDDATTNEKGAVLIEVLANDFDNDPKDAPRV
                     IGIDDAGLVGRVTLNDDGTVLYETNGQFDHLGQGESVQETFTYTVADRFGATSKATVT
                     VEVTGVNDAPEAAGDRAVTNQNWPVRIDVIGNDADVDANDTIRVVSVDTTGLVGSAFV
                     NHDGTLTYRPNDQFDHLGKGEKAFETFSYTIADQHGATSTASVTVQINGVNDAPVAHA
                     DHTQVNENWPVRIDVLANDTDVDVNDTLRVVSVDTTGLVGDAFVNHDGTLTYRHNGAF
                     DYLGEDEVAYETFTYTVADQHGANSTASVTVKIIGCNDAPVAADDHATTDEDTVAVFN
                     LLANDTDVDATDVLRVVEVDASALAGSLTVHPDGTATYDPRGSFDHLRAGQTAHESFS
                     YTISDSHGETSTATVELTIEGVNDAPVAGTDFFTGYETVGFFASLASLVANDSDVEGD
                     PLSILDVGNAVNGTVGLTPDGYVVFTPTSGYTGLAGFQYLLADGEGGYALGDVYVDVL
                     PITPGVVTYETLSLFEAIGNANASLAEDGTTQLDLYLEVASVAGTNVEIGGMPYFTAV
                     MTGILQGVLSVTIGPDGTPQATGNLTADLNLELTQSPIFRPLGGDLYDALYLASGSSG
                     PVSLPGNDPLAPPDIADLLGGSSLAALLEDALNLLDLGGAVVEIGGLGLGQATSIAFG
                     DGTFYLDISGLISGTIAVAGVNDNQPLALAELFGQFAGAAQIDIREGLAVASDGFVDG
                     DFQVQLASTGLDGGLSIGSMVSEEPALAA"
BASE COUNT         1538 a         3212 c         2983 g         1390 t
ORIGIN      
        1 gggcgatcga ggtggccggc atcggcagcc gcgaggatct ctactggacc ctgcacgcgg
       61 tcttcgtgaa ccggcgcgat cagcgcgagc tgttcgacca gtgcttccac atcttctggc
      121 gcgatccgca gctgctcgag cggctgatgc acctgatgct gccgacgctc cgccatgatc
      181 agcccggcga ggaggaggag ctcagccgcc gcgtggccga ggcgctgacg ccggagaagc
      241 agccgggccg gggcgaggcg ccggaggacg agaaggagga agagatcgag ctcgacgccg
      301 tgctcacgta ttccgccaag gagctcctgc aggagaagga cttcgaggag atgtcggctg
      361 acgagctggc ggacgccaag cgacagatcg agcggctgcg cctgcccatc gtcgccttgc
      421 cgacccgccg tttccagggc gacccgcacg gccggcggat cgacctgcgt cgcacgctcc
      481 gtcaatcgat gcgcggcggc ggggcggtga tcgacctcgc ccgcaagaag cgcgtgcggc
      541 gtcacccacc gctggtcatt ctttgtgaca tttccggctc gatgagccgc tattccagga
      601 tgctgctgct cttcatgcac gccctgaccg gcgatcgcga ccgggtccac agcttcgtct
      661 tcggcacgcg gctgaccaac atcacccgat acctgcgcaa caaggacgtg gacatcgccc
      721 tcgaccgggt cggcgaggtg gtcaaggact gggccggagg gacccgcatc ggccactgcc
      781 tcgaggcgtt caaccgcgac tggtcgcgcc gggtgctggg ccagggtgcc gtggtcatgc
      841 tgatcaccga cgggctcgac cgcgatgccg gcgacgggct ggagcaggag atggagcggc
      901 tgcacaagtc ctgccgacgg ctgatctggc tcaatccgct gttgcgctac gagggcttcc
      961 aacccatctc atcaggagcc cgggccatgg tggcgcacgt cgacgatttt cgcaccgtgc
     1021 acaacctgaa cagcctgcgg gaccttgccg acgtgctgaa tcgcgagccg caccggcggg
     1081 ccgagggtgt ttcggtctgg gcggccgagc aggaggagac cggagcccat gctggatgac
     1141 gtcgtgctgg atgatacctt gctggacagc gccgccgagt ggcacgcagc cggccgcagg
     1201 gtggcgatcg ccactgtggt ttcgacctgg ggctcgagcc cgcgcccgcc cggcagccag
     1261 ctcctgatcg atgaccaggg caacatgttg ggctcggtct cgggtggttg catcgagggc
     1321 gctgtggtca agaaggctct cgacatcatc gatggtgcgc cgcccgaggt catggagttc
     1381 ggtgtctcga acgagcaggc ctgggaagtg ggcctcgcct gcggtggccg ggtccaggtc
     1441 tatgtcgaag ctctgtccta ggaatctgcc gtgcgccctg ccaccctcga actgctgaac
     1501 gaggcccgcg ccgccaagcg ccacgcctgc ctcgttcgca atctggacag ccacgcggaa
     1561 gcgctggtcg tcgacggcga agtaatgctc ggatcggtga gccccgcgct gctcgccgag
     1621 gtggaagcct gcctgcgggc cgatcgctcg agaccggtcg aagtccccga gggccgggcc
     1681 ttgctccaga tcttcaaccc gcccttgcgg cttgccatca taggcgccgt gcatatcgcg
     1741 caaatgctgg caccgatggc cgccatcgcc ggctacgccg tgacggtgat cgatccgcgg
     1801 ggcgccttcg cgaccgagga gcggttcccc ggtgtgacgc tgtcgcacga ctggccagac
     1861 gaggcaatgg aggccttcgc acccgaccgg cggtcggcga tcgttgccct gacccacgat
     1921 cccaagctgg acgatccagc actcgacgag gcgctgcgga gcgacgcctt ctacatcgct
     1981 gccctcggca gcaagcgaaa ccatgccgcc cgcatgggcc gcctcaggga actcggccac
     2041 accgatcagg cgattgcccg gatccacggg ccggccggcc tcgatatcgg cgcggtgagc
     2101 ccggcggaga tcgcgatttc cgtcatggcc cagatgaccg ccgtcctgcg ggagccgcgt
     2161 gacgtcgcat cccggggcga agcagcatga agttcggcac gattccagtc gacgaagcgg
     2221 aaggagcact gctcgcccac agcattcggc tcgatgggga gacgctgaaa aagggacgcc
     2281 tgcttaccgg ggatgacctc gacaccctgc gggacgccgg catggcaacg gtggtcgcgg
     2341 cacggctcga gccggacgac gtgcccgagg acaccgccgc cgcccggctg gccatgaccc
     2401 ttggcgggcc agcaatccgc gtcgccgagc cctttaccgg tcgtgccaat gtcttcgccg
     2461 agaccgacgg cgtgttcaca gtcgacaagc gcacgatcga tggggtcaac ggcctcgacg
     2521 aggccatcac catagcgacc gtcgcggatc atgccgccgt gagcgagggc cagatgctgg
     2581 ccacggtcaa gatcatcccc ttcgccgccc ccgaggccgc ggtgaaccaa gccctggcca
     2641 tcctgaacga ccgggcgccg gtgctccgcc tgcacccgtt ccggccgctc gtggcacgcc
     2701 tgatccagac gtccctgcca gacaccaaca cgaagatgct cgacaagacg gcgcgcatca
     2761 ccggggaacg gatggccgcc gtgggcggca gcctcgcgag cgaaagccgc tgcgggcaca
     2821 cgaccgaggc gctggccgac cagatcacgg gggcgctggc ccagggctgc gatctcctgc
     2881 tcatcgccgg tgcatcggcg atcaccgacc ggcgcgacgt gctgccggcc gggatcgagg
     2941 cggtcggcgg caggatccgt catttcggca tgccggtcga tcccggcaac ctgctcctgc
     3001 tcgccgcgct cgatgatgtg cccgtgctgg gcttgccggg ctgtgcccgc tcgcccaagc
     3061 tcaacggctt cgactgggtc ctgcagcggc tggcggccgg tctcgaggtc gaccgtgcca
     3121 ccatcatggg catgggcgtc ggcggcctgc tgaccgagat cccgacccgg ccgcagccgc
     3181 gaacgcgcaa gcggtcggtg cgggacgagt cgcggatcgc cgttctcgtc ctggctgccg
     3241 gccagtcgcg ccgcatgggg ccgaccaaca agctgctgat cccgatcgat ggcaagccga
     3301 tgctgcggca cacggtcgag gccgccctca cctcgaaggc caacgacgtg attgtggtca
     3361 ccggccacga gcacgcggcg gtcgaggcca tcctggccgg cacggccgtg cgcatcgtgc
     3421 acaacccggc cttcgcagac ggactcagca cctcactcaa ggccggaatc gccgccttgc
     3481 cgccgggcat cgacgcggcg ctcgtctgtc tcggcgacat gcccgaaatc gatgccgagc
     3541 tgatcgaccg gctgatcgcc gccttcgagc caggcagcgg gcggtcgatc gtggtcccca
     3601 cccacaacgg caagcgcggc aatcccgtgc tctggggcag cgccttcttc ccggcgatgt
     3661 tggccatcga gggcgatgtc ggtgctcgcc atctgatcgg ccagcatgcc gaggcggttg
     3721 tcgaggtcgc cttcgacaat gcctcgagcc tcgtcgacat cgacacgccc gaggcgctcg
     3781 aagcctatct gactggacag gattgatggg agtcggccga gagctgcggc cgcagttccc
     3841 gatttttgcg agctttccag agcctctcca ctatctcgac aacgcggcga ccggacagat
     3901 ctgcgccgcg gcggcagaag cgctctggga gttcgagaca gcggcccgcg ccaatgtcaa
     3961 acgcggcgtc taccgtctgg ccgaccaggc atcggctgct ttcgaagccg cccgctcgac
     4021 catggccagc tatatcgggg caccggctcc cgacgaggtg gtcttcacct cgggcgccac
     4081 gtccgccctc aacatcgcgg cgcacggtct cgcggaacgg ctgtcgccgg gcgacgagat
     4141 cctcgtctcg gtgctcgagc atcacagcaa catcgtcccc tggcagctcg cggccaaggc
     4201 caggcaggcg gtggtcaagg ctattccggt gacggacgac ggccgcctcg atctcgatcg
     4261 cctcgggggc gtcctgaccg atcggaccag ggtcgtctcg ctcacccacg tctccaacgt
     4321 gaccggagcg gtgaccgacc tggcacggct ccgggccgcc gccgacgacg tgggggcgat
     4381 tctcgtggtc gacggcgccc agcgtgcccc gcacggcccg atagacgtca aggcgatcgg
     4441 cgccgatctc tacgccttct cggcgcacaa gatgttcggc gccaccggcg ccggcgtgct
     4501 ctggggcaga tcggcgatcc tcgatgcctt cccgccgctg ctcggcggcg gcgaaatgat
     4561 ccgcgaggtc agcttcgccg gcacgacctt cgccgccccg ccgcaccgct tcgaggccgg
     4621 cacgccgcca atcggcccgg tgctggccat ggccgccgcg gccgagtggc tcatggcgca
     4681 ggactggtcg gcgctggccg cacatgaggc gaggctgacc ggccgcctcc tcgacggcct
     4741 cggctcgctt cccggcgttc gcatcctcgg ccccaccggg ctgcagcagc gcttgggcgt
     4801 ggtcgccttc gaggtggatg gcgttcacgc ccatgacgtc tgccagctcg tcgaccagct
     4861 tgccggcgtc gccctgcggg gcgggcacca ctgctgccag ccactgatgg agcgcttcga
     4921 cacgatcgcc acggcgcgcg ccagcctcgc cccctacaat gacgacgacg atatcgacgc
     4981 gctcatcgac ggcctggagc gcaccatcga gatgctgcaa tgaacgaggc tctctacaac
     5041 cgggcgatca tcgaggcggc ccgcgacaag cgccatgcgg ggcggctgga gcatcccggc
     5101 ggcacggcca cgacggacaa cccgctgtgt ggtgaccggg tcacgctgga tctcgagggc
     5161 gaggcgggac gaatcgcggc ggtcgcccac aagacgcgtg gctgtctctt gacccaggcc
     5221 gcggcatcgc tgctcgccgc tcatgccgtc ggccgtccga tcgatgaggt ggtgccttta
     5281 cgtggcgcca tccgggcata tctcgatggg acagcggagg acccgccctt cccggagctc
     5341 gccatgctgg ccccggtgcg tgccgtcaag agccggcacg agtgcgtcac catagccttc
     5401 gatgctctgg cagaggcggc cgttcaactt gccacggaga aaccgtcatg aatgacaagg
     5461 tcgtcaagga cgatgccgag tggcgcgcca gcctcgatcc ggagcagtat cgtgtcgcgc
     5521 gcgagcaggg caccgaacgg ccgttcaccg ggcgctactg gaataccaag gaagccggca
     5581 cctacgcctg cgtctgctgc ggccagccgc tcttcgccag cacgaccaaa tttgacagcg
     5641 gcaccggctg gccgtcgttt tgggcgccga tcgacgaaga ggccgttgcc acaaggacgg
     5701 atcacagtca cgggatgacc cgcaccgagg cactctgcgc cagatgcgac gcccatctgg
     5761 gccatgtctt tcctgatggc cccgagccca ccggcctgcg ctactgcctc aactccgcat
     5821 cgctcgacct gaagccgcag agcgactgac gagcgggggc aggtcactcc tgcccccggt
     5881 ttaccatctt tcgtccgaac ccttcgaacg taaacaagaa tagtaaataa caaacagatc
     5941 tatttctgcg tacaataata agaagttcgt aaacggatct cgcttattaa caaagattcg
     6001 gcacagcgcc ttcgattaca aagaagctgt tgatcggtcc cgaatgaacg ccggcgcaaa
     6061 ttgcaatggc atcgggtgtc tcgatcgaca cgacttcctg gtgatcgatt gcaagaggac
     6121 gccatggccg aagccccgaa cagcccagtc gccggcgaga ccccttcgat gccggccgga
     6181 aatgcacagc cgcagggtgt cgatgaaagg cgggtgacac cggcttcgga cacggacacc
     6241 ggcgacgccc gcgcacccgt cgtcatcgac ctgacggaag tggacacggc cggcgccacc
     6301 ttcgagctga acgaggcgcg gctgatcctg cgcttcgccg acggccagga gcagacactc
     6361 gcggaattgc cccttcagga accggcccat gtccggctcg cggatggcgg cgccatcgag
     6421 cttggggcgc tcgccgaggc gctgaccgct gccgatggcc ccctgccggt tcccgcggcg
     6481 attgccgagc tcgccgaggc cgcccccgag gcgggcgcgg agccggtgag cggcggtgcc
     6541 gagttccgac tgatgcagat cgacgatctc ggcgcctccc ccgatccgac gatgcagctg
     6601 ggtgccgtgg aatacggcag gacgctcggc ggcgactccg agttcttcac ccgcagcggg
     6661 gcgctcgacg gcagcgggcc gctcggcagc accggcaatg gcggccccgg tggcggcggt
     6721 ctcggatccg gcccgaatgg tggcggcttc atcaacaatc cgcccgtcgc ccgtcatgac
     6781 gacgcgacga cgaacgagaa gggcgccgtc ctcatcgagg tgctcgccaa cgacttcgac
     6841 aacgacccca aggatgcgcc gcgcgtcatc ggcatcgacg acgccgggct cgtgggccgc
     6901 gtgaccctca acgacgatgg caccgtgctc tacgagacca acggccagtt cgatcatctg
     6961 ggccagggcg agagtgttca ggagacgttc acctacacgg tggccgaccg cttcggggcc
     7021 accagcaagg cgaccgtcac cgtcgaggtg accggcgtca acgatgcgcc cgaggccgct
     7081 ggcgacaggg ccgtcaccaa ccagaactgg ccggtcagga tcgacgtcat cggcaacgat
     7141 gccgatgtcg acgccaacga caccatccgg gtggtgagcg tcgacacgac cggcctcgtc
     7201 ggtagcgcct tcgtcaatca cgacggcacc ctcacctacc gtcccaatga ccagttcgac
     7261 catctcggca agggcgagaa ggccttcgag accttcagct acaccatcgc cgaccagcac
     7321 ggcgccacca gcaccgccag cgtcactgtc cagatcaacg gcgtcaacga cgcaccggtg
     7381 gcgcatgccg accacaccca ggtcaacgag aactggccgg tcaggatcga tgtcctcgcc
     7441 aacgacacgg acgtcgacgt caacgatacg ctgcgcgtcg tgagcgtcga tacgaccggc
     7501 ctcgtgggcg acgccttcgt caatcacgac ggcaccctga cctaccggca caatggtgcc
     7561 ttcgactatc tgggcgagga cgaggtcgcc tacgagacct tcacctacac ggtggccgac
     7621 cagcacggcg ccaacagcac cgccagcgtc accgtcaaga tcatcggctg caacgacgca
     7681 cctgtggccg ccgatgacca cgccaccacc gacgaggaca cggtcgccgt cttcaacctc
     7741 ctggccaacg acaccgatgt cgacgcgacc gacgtgctgc gcgtcgtcga ggtcgacgcg
     7801 agcgcgctcg ccggcagcct caccgtccat ccggacggca cggccaccta cgatccgcgg
     7861 ggcagcttcg accatctccg cgccggccag acggcgcacg agagcttcag ctacacgatc
     7921 agcgacagcc atggcgaaac cagcaccgcc accgtcgagc tgacgatcga aggcgtcaac
     7981 gatgctccgg tcgcgggcac cgacttcttc accggctacg agacggtcgg cttcttcgcc
     8041 tccctcgcca gcctcgtcgc caatgacagc gacgtggaag gcgatcccct gagcatcctc
     8101 gacgtcggca acgcggtgaa cggcacggtc ggcctgaccc ccgacggtta cgtggtgttc
     8161 acaccgacgt ccggctacac tggtttggcc ggcttccagt atctcctcgc cgatggcgag
     8221 ggcggctatg ccctgggcga cgtctatgtc gacgtgctgc cgatcacgcc gggtgtggtc
     8281 acctacgaga cgctgagcct gttcgaggcc atcggcaacg ccaatgcctc gctcgccgag
     8341 gatggcacga cccagctcga cctctatctc gaggtcgcct cggtcgccgg cacgaatgtc
     8401 gaaatcggcg gcatgccgta cttcacggcg gtgatgaccg gcatcctgca gggcgtcctt
     8461 tcggtgacga tcggcccgga cggcacgccg caggcgaccg gcaatctcac ggccgacctg
     8521 aacctcgagc tcacccaaag cccgatcttc cggccgctcg gcggtgatct ctacgacgcg
     8581 ctctacctcg cctcgggctc gtccggaccg gtcagcctgc cgggcaacga tcccctggcg
     8641 ccgcccgaca tcgccgacct gctcggcggc tcgtcgctgg ccgcgctcct cgaggacgcg
     8701 ctgaaccttc tcgatctcgg cggcgcagtg gtcgagatcg gcggtctcgg cctcggccag
     8761 gcgacgtcga tcgccttcgg cgacggcacc ttctatctgg acatctccgg gctgatcagc
     8821 ggcacgatcg ccgtcgcggg cgtcaacgac aaccagccgc tggccctggc ggaactcttc
     8881 ggccagttcg cgggcgccgc ccagatcgac atccgtgaag ggctcgccgt cgcctcggac
     8941 ggcttcgtcg acggcgactt ccaggtccag ttggcctcga ccggcctgga cggcggcctc
     9001 tcgatcggct ccatggtcag cgaggagcct gccctggccg cctgaacggt ccaacgtcgc
     9061 ggaccaaggg aagggctggc gccacgcgcc ggcccttccg ccgttcaggg caggaacggc
     9121 ccc
//
LOCUS       DAOTFI010000198         9060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9159_length_9060_cov_7.282288, whole genome shotgun sequence.
ACCESSION   DAOTFI010000198 DAOTFI010000000
VERSION     DAOTFI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9159_length_9060_cov_7.282288"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..343)
                     /locus_tag="P5558_17810"
     CDS             complement(170..343)
                     /locus_tag="P5558_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26231.1"
                     /translation="MSPDQLFGLVAMTALLIWLGRGFLPHRHHKTMERLAMALIGGGI
                     LVALAFTVLHFSS"
     gene            complement(340..1494)
                     /locus_tag="P5558_17815"
     CDS             complement(340..1494)
                     /locus_tag="P5558_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY26232.1"
                     /translation="MSEPAPIRVGLIGSGFMGRSHAIAMRTAQAVFGLERPLVPTVLA
                     DVDAATAKHAGAALAIDRTTGDWRSLVDDAEIDLIDITTPNTLHEPMAMAAIGAGKPV
                     YCEKPLAPDAATACRMALAAEAAGVPTFVGFNYLKNPLVQTARAMVESGEIGEVWGFR
                     GIHAEDYMTDPAAPWTWRLDPRGGAGAVADLGSHIISLARHVVGPITDLSADLSTKIR
                     ERTTAAGERRPVQVDDEARALIRFAGGATGTLEASWLATGRKMYLAFELTGSKGSILL
                     NMERMNELRLFVRGGVAGREGYVEIPAGPAHPDYAPFCPAPGHQLGFNEIKAIEVKAI
                     ALALAGGAAFQPDFREAAMIQATVDTILRSARERRWVAVDEVLPKGFVAG"
     gene            complement(1491..2468)
                     /locus_tag="P5558_17820"
     CDS             complement(1491..2468)
                     /locus_tag="P5558_17820"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRY26233.1"
                     /translation="MADVLVIGTIRPEGLAVLEGRADIRLTKLAETDRAGLLEAAGRA
                     SAILVRTSRVDRAVIEAAPGLKVVSRHGVGYDNVDLEALNERRIPLAIAANANKVSVA
                     EHALFMMLALAKQGVAHDTAVRGGEWAIRNRFAAVDLAGRTLLLLGFGRIGGEVAVRA
                     RAFGMTVEVFDPYVDDRSLAECGCRRATELESALREADVVSLHLPLSAATRHIIDAAK
                     LALMRPTALLINTARGGLIDEPALARALTEGRLGGAGIDVFEDEPPDPANPLLAAPRA
                     LLSPHSAGVTGESMVRMATEAATNILDVLGGRLDPAVIVNHAIVAGSQP"
     gene            complement(2473..3261)
                     /locus_tag="P5558_17825"
     CDS             complement(2473..3261)
                     /locus_tag="P5558_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY26234.1"
                     /translation="MRENRLRQLWDAGEKTVNAWLGIPNSFSAEIMAQQGFESVTIDM
                     QHGLVDYPASIGMLQAISTTDAVPLVRVPWNEPGIIMKSLDAGAYGIICPMINSKAEA
                     EAFVSSVRYPPLGSRSFGPIRATMYAGADYYKHANNTVLTLAMIETTDAMAALEDILA
                     VPGLDGIYVGPADLSISMGHAPRMDQEDQPVVDAIQKIIDTTKRHKKYAGIHCGSVSY
                     IKKMWGIGFDYATLLSDARMLAMKAGELLPELQAKSSGGKGVGY"
     gene            complement(3601..4467)
                     /locus_tag="P5558_17830"
     CDS             complement(3601..4467)
                     /locus_tag="P5558_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRY26235.1"
                     /translation="MQQDNPKKRGLGMGLSALLGNDAPDPSLRPLAQSVPIEFLSASP
                     LQPRRHFADDELEALAASLQAYGVLQPLLVRPRPGSAPGYEIVAGERRWRAAQRAGLH
                     ELPVVVRELADRDVLEIALVENLQREDLTPIEEARAYQRLIDEFGRTQEEIAREIGRS
                     RPHIANTLRLLKLPEPVRRMLEDGRLTAGHARALLAADDPVALAQAVVERGLNVRDTE
                     AVVQTGKLVQRQARGAIERDPDLEAFESRLASTLGLACRLQPKAKGGTLTIRYSSLDQ
                     LDRLASRLLATD"
     gene            complement(4502..5335)
                     /locus_tag="P5558_17835"
     CDS             complement(4502..5335)
                     /locus_tag="P5558_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY26236.1"
                     /translation="MSAPPRQTAAPARSSVLCGRILAIANQKGGVGKTTTAINLGTAL
                     AAAGKRILVIDFDPQGNASTGFGIDREARRVTAYEVVLGQARLADAILPTMVPGLDIV
                     PATVDLSAAEVELVGDARRNFRLRDAFANFIKPYDHILIDCPPSLGILTVNALAAAHA
                     VMVPLQCEFFALEGLSMLLRTIERIRQSLNPMLQIHGIVLTMFDRRNNLSEQVATDVR
                     SFLGDRVYQTVIPRNVRISEAPSHGQPVLYYDVKSSGAKAYAHLAGEMLRRDQAIAVF
                     G"
     gene            complement(5341..5970)
                     /gene="rsmG"
                     /locus_tag="P5558_17840"
     CDS             complement(5341..5970)
                     /gene="rsmG"
                     /locus_tag="P5558_17840"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRY26237.1"
                     /translation="MRREAPLDAAGFAAQVPVSRETVERLTLYLELLRQWQKAINLVG
                     ASTLDDAWRRHILDSAQLFRHLPAGCATLADLGSGAGLPGLVLAAMGVPEVHLVESDR
                     RKAQFLREAARRMGLAGVTVHPVRIETLSLAVDVVTARALAPLVQLVPLARPLLRAGG
                     RLVLLKGRGVDAELTALGEVGTMRVELHPSLADPEGRVLVLQEVASSAP"
     gene            complement(5960..7810)
                     /gene="mnmG"
                     /locus_tag="P5558_17845"
     CDS             complement(5960..7810)
                     /gene="mnmG"
                     /locus_tag="P5558_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRY26238.1"
                     /translation="MESHDVIVIGGGHAGAEAAAASARMGARTLLLTQKLATIGAMSC
                     NPAIGGLGRGHLVREIDALDGLMGRAIDAGGIQFRVLNRRKGPAVQGPRAQADRRLYA
                     RAVQDLLAAQAGIVLAEGEAAGLLRHGTRVTGVVTAEGRELRAGAVVLTTGTFLRGEI
                     HLGTERWPAGRVGDQPAVALARDLEAAGVRLGRLKTGTPPRLDGRTIDWAALARQPGD
                     EPPRPFSFLTTRITNPQIACHVTRTTEASHDLIRAHLHEAPLFSGQIAATGVRYCPSI
                     EDKVVRFAERTGHQVFLEPEGLDDHTVYPNGISTSLPRAVQEALVRTIPGLGRAVITQ
                     PGYAIEYDHVDPRELDPTLELRALPGLFLAGQINGTTGYEEAAAQGIMAGANAALRAS
                     GRRPDAVLDRAEAYIGVLVDDLTTQGITEPYRMFTSRAEYRLMLRADNADRRLTERGT
                     GLGLVGTARHARLLDKLDKLTRAEALLDDHRLTPPEAATYGIRVNHDGATRTARQLLA
                     SPEVDIARLATIWPELGDIAAEIAEQIEIGERYAPYLKRQVADIADFRREESLDLPPE
                     VDFAALAGLSGELRALLSRVRPTTLGAAARLPGMTPAALTLLYRHVRRAA"
     gene            7901..>9060
                     /locus_tag="P5558_17850"
     CDS             7901..>9060
                     /locus_tag="P5558_17850"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY26239.1"
                     /translation="MSGIGGVILGFGVLLALIAPLPPLAERLRLPYTVLLAILGCCLG
                     LLTGFAARFEEMLIDSDVLDLVAAISELEVGSAVFLWVFLPLILFHSAMELDSRALLE
                     DIGPVILLAVVAVLATTLIAGLAIWSVSTVTLASCLLVGAIIATTDPIAVINVFQEVG
                     APRRLVSLVEGESLLNDAAAIALTTSLLALVVGLAAPSFETIVGDFVVSMAGGAVFGL
                     VVGRLAGLLLTRLDEAGPAEITLSVAVAYLTYAIAELQFGISGVVAVVAAGIVFGAQG
                     RAGLSRADWRSLIAIWAQLSFWASSLIFVLAAMLAPKTLATAGPFDAVLLVLLTLGAL
                     GSRAMILYGALPLMEAIGLAGRIQTSFKHVMLWGGLRGAITLALALAVTENPL"
BASE COUNT         1277 a         3081 c         3274 g         1428 t
ORIGIN      
        1 ggggcgtgtt cctgcaatgc acgtcgttcc tgcccttcgc cgccatcgtc gagggccgcc
       61 agcgcttcgt ctgggccgag atcggctggg cgcggctggg tgcggcgctc ctcctctacg
      121 cgctccttgt ggccccgcat ccctggcttt tcggggtgcc ggccatcggc tagctcgaga
      181 agtgcaggac ggtgaaggcg agggccacga ggatgccgcc gccgatcagc gccatcgcga
      241 ggcgctccat cgtcttgtgg tggcggtgtg ggagaaagcc ccggccgagc cagatcagca
      301 gcgcggtcat ggcaacgagg ccgaagagct ggtccgggct caccctgcga cgaaaccctt
      361 cggcagcacc tcgtccacag ccacccagcg gcgttcccgg gcggagcgga ggatggtgtc
      421 gaccgtcgcc tggatcatcg ccgcctcgcg gaaatccggc tggaatgcgg ccccgccggc
      481 gagggccagc gcgatcgcct tgacctcgat cgccttgatc tcgttgaagc cgagctggtg
      541 cccgggtgcg gggcagaagg gcgcgtaatc gggatgcgcc gggccggccg ggatctcgac
      601 atacccctcg cggccggcaa ccccgccacg aacaaagaga cgcagctcgt tcatccgctc
      661 catgttgagc aggatcgagc ccttcgagcc cgtcagctcg aaggcgaggt acatcttgcg
      721 gccggtggcg agccaggagg cctcgagcgt gcccgtggcg ccgccggcga agcggatcaa
      781 tgcccgcgcc tcgtcgtcca cctggactgg ccggcgctcg ccggcagcgg tcgtccgctc
      841 cctgatcttg gtcgagagat cggcggagag atcggtgatg gggccgacga catggcgggc
      901 gagcgagatg atgtggctgc cgagatcggc gaccgcgccg gccccgccgc gcggatcgag
      961 gcgccaggtc cagggtgccg ccgggtcggt catgtagtcc tcggcgtgga tgccgcggaa
     1021 gccccagacc tcgccgatct cgccgctttc gaccatggcc cgggccgtct ggacgagcgg
     1081 gttcttgagg tagttgaagc cgacgaaggt cggcacgccc gccgcctcgg cggccagcgc
     1141 catgcggcag gccgtggcgg cgtcgggggc cagcggcttc tcgcaataga cgggcttgcc
     1201 cgccccgatg gcggccatcg ccatgggctc gtgcagcgtg ttcggcgtgg tgatgtcgat
     1261 gagatcgatc tcggcatcgt cgacgagcga ccgccagtcg ccggtcgtcc ggtcgatggc
     1321 gagggccgca ccggcgtgct ttgccgtggc cgcgtcgacg tcggcgagga cggtcggcac
     1381 cagcggccgc tcgagcccga agaccgcctg ggccgtacgc atggcgatgg catggctgcg
     1441 ccccatgaag ccgctgccga tcaggccgac acggatgggg gcgggctcgc tcatggctgt
     1501 gatccggcga cgattgcgtg gttgacgatg acggcgggat cgagccggcc gccgagcaca
     1561 tcgaggatgt tggtcgccgc ctcggtcgcc atgcgcacca tggattcgcc ggtcacaccg
     1621 gcgctgtgcg ggctgagcag ggcccggggt gcggcgagca gcgggttcgc agggtcgggc
     1681 ggctcgtcct cgaagacgtc gatcccggcc ccgccgaggc gtccctcggt gagcgcccgg
     1741 gccagggccg gctcgtcgat gagcccgcca cgggcggtat tgatcagcag ggccgtcggg
     1801 cgcatcagtg ccagcttcgc agcgtcgatg atgtgacggg tggcagcgct cagcggcagg
     1861 tgcaggctga ccacgtcggc ctcccgcagc gccgactcga gctcggtggc gcggcggcag
     1921 ccgcactcgg cgagacttcg atcgtcgaca tagggatcga agacctcgac cgtcatgccg
     1981 aaggcgcggg cgcggaccgc cacctccccg ccgatccgcc cgaagcccag aagcaagagc
     2041 gtgcggccgg cgagatcgac ggcggcgaaa cggttgcgga tcgcccactc gccgccacgc
     2101 acggcggtgt cgtgggcgac gccctgcttc gccagcgcca gcatcatgaa gagcgcgtgc
     2161 tcggcgaccg agaccttgtt ggcgttggcg gcgatggcga gcgggatgcg ccgctcgttc
     2221 agcgcctcga gatcgacatt gtcgtagccg acgccgtgtc gcgagacgac cttcagcccc
     2281 ggcgccgcct cgatgaccgc acggtcgacc cgcgaggtcc ggacgaggat ggccgatgct
     2341 cgaccggccg cctccagcag gccggcacga tcggtctcgg cgagcttcgt caggcggatg
     2401 tccgcacgac cttcgagaac cgccagccct tcgggccgaa tggtgccgat gacgaggaca
     2461 tccgccatgg catcagtagc cgacgccctt gccgccgctg ctcttcgcct gcagctcggg
     2521 cagcagctcg ccggccttca tcgccagcat gcgcgcatcc gagagcagcg tggcgtagtc
     2581 gaagccgatg ccccacatct tcttgatgta gctcaccgag ccgcaatgga tgccagcgta
     2641 cttcttgtgc cgcttggtcg tgtcgatgat cttctggatg gcgtcgacca cgggctggtc
     2701 ttcctggtcc atcctgggcg cgtggcccat cgagatggag agatcggcgg gcccgacata
     2761 gatgccgtcg aggccgggca cggcgaggat gtcctcgagc gccgccatcg cgtcggtggt
     2821 ctcgatcatg gcgagggtga ggacggtgtt gttggcatgc ttgtagtagt cggcgccggc
     2881 atacatcgtc gcccggatcg gcccgaagct gcggctgccc aatggcgggt agcggaccga
     2941 cgagacgaag gcctcggcct ccgccttgct gttgatcatc gggcagatga tgccgtaggc
     3001 gccggcatcg agggacttca tgatgatgcc gggctcgttc caggggacgc ggacgagcgg
     3061 caccgcgtcg gtcgtcgaga tcgcctgcag catgccgatc gacgccgggt agtccaccag
     3121 cccgtgctgc atgtcgatgg tcacgctctc gaagccttgc tgggccatga tctcggcgct
     3181 gaagctgttt ggaatgccga gccaggcatt gaccgtcttc tcgccggcat cccagagctg
     3241 ccgcagccga ttctcacgca tgtttcccgt cccccttgaa tggcgagccg gaacctatgt
     3301 cgcggggcgg tcgctgtcca gcgcgtggca gcgacctcga ggcggctgga cgaacgaacg
     3361 ccgcccccgg gcgggggcgg cgtcaatcgg gagagggacg ggtgccgatt ggctcggctt
     3421 gtccgtcgag cagcgggacg ttccgattga tcggtgcctg cagcccgtct gggcagagac
     3481 ggtgggaccc tggaggaccg gatcagggaa tgaactcttt ccttgtttca ccccgacgcg
     3541 ataaggccac aagatatgct cgtggtcaac ctgcctggca caaattctag tggataactt
     3601 tcaatcagtc gccagcaggc gcgaggcgag gcggtcgagc tggtcgaggc tcgagtagcg
     3661 gatggtgagg gtgccgccct tggccttggg ctgcaggcga caggcgaggc cgagcgtgct
     3721 ggcgagccgg ctctcgaaag cctcgagatc gggatcgcgc tcgatggccc ccctcgcctg
     3781 acgctggacc agcttgccgg tctgcaccac cgcctcggtg tcgcggacgt tgagaccgcg
     3841 ctcgaccacc gcctgggcca gcgccaccgg gtcgtcggcg gccagcaggg cgcgggcatg
     3901 gccggccgtg aggcgcccgt cctccagcat gcggcgcacg ggctccggca gcttcaaaag
     3961 gcgcagcgtg ttggcgatgt gcggccgact acggccgatc tcccgggcga tctcctcctg
     4021 ggtccggccg aactcgtcga tcagacgctg gtaggccctg gcctcctcga tcggtgtcag
     4081 gtcctcgcgc tgcaggttct cgaccagggc gatctcgagc acgtcgcggt cggcgagctc
     4141 gcgcaccacg accggcagct cgtgcagccc ggcccgctgg gccgcccgcc agcggcgctc
     4201 gccggcgacg atctcgtagc ccggcgccga ccccggtcgc ggccggacga gcaaaggctg
     4261 cagcacgcca taggcctgca ggctcgccgc cagcgcttcg agctcgtcat cggcgaaatg
     4321 ccggcggggt tgcagcggcg aagcgctcag gaactcgatc ggcacggact gggccagcgg
     4381 tcgcaggctc gggtccgggg cgtcgttgcc gagcagcgcc gaaaggccca tgccgagccc
     4441 gcgcttcttg ggattgtcct gctgcatccg gtgggaatcc ttcttgggca gttggggtcg
     4501 gtcagccgaa gacggcgatg gcctggtcgc gtcggagcat ctcgccggcg agatgggcat
     4561 aggctttcgc accgctcgac ttgacgtcgt agtagagcac cggctggccg tggctcgggg
     4621 cttcggaaat gcggacattg cgcgggatga cggtctggta gacccggtcg ccgagaaagc
     4681 tgcgcacgtc ggtggccacc tgctcggaaa ggttgttgcg gcggtcgaac atggtgagca
     4741 cgatgccgtg gatctgcagc atcgggttga gcgactggcg gatgcgctcg atggtccgca
     4801 gcagcatcga caggccctcg agagcgaaga actcgcattg cagcggcacc atgacggcat
     4861 gcgccgcggc gagcgcgttg accgtgagga tgccgagcga gggcgggcag tcgatcagga
     4921 tgtgatcgta gggcttgatg aagttggcga aggcatcccg cagccggaaa ttgcggcgcg
     4981 catcgccgac cagctcgacc tcggcggccg agaggtcgac cgtcgccggg acgatgtcca
     5041 gccccggcac catggtcggc aggatcgcgt cggcgaggcg ggcctggccg agcaccacct
     5101 cgtaggcggt gacccgccgc gcctcgcgat cgatgccgaa accggtgctg gcattgccct
     5161 gcgggtcgaa gtcgatgacc aggatgcgct tgccggcggc ggccagcgcc gtgccgagat
     5221 tgatcgccgt cgtggtcttg ccgacgccgc ccttctggtt ggcgatggcg aggatccggc
     5281 cgcacaggac cgaggaccgg gcgggggctg ccgtctggcg tggcggggcg gacatctggc
     5341 tcagggcgct gatgacgcca cctcttggag gacgagcacg cgaccttccg ggtcggcgag
     5401 gctcggatga agctcgaccc gcattgtccc cacttcgccg agcgcggtca attccgcgtc
     5461 gacgccgcgg cccttcagca gcaccagccg cccgcccgcc cgcagcagcg gccgggccag
     5521 aggcacgagc tggaccagcg gggctagtgc ccgcgccgtc accacgtcga cggcgagcga
     5581 cagggtctcg atccgcacgg gatgcacggt gacgccggcg aggcccatgc gccgcgccgc
     5641 ctcgcgcagg aactgcgcct tgcgccgatc ggattcgacg agatgcacct ccggcacgcc
     5701 catggcggcg agcacgaggc cgggcagccc ggcgccgctg ccgaggtccg ccagcgtggc
     5761 gcaacccgct ggcagatgcc ggaagagctg ggcggaatcg aggatgtgcc gacgccaggc
     5821 atcgtcgaga gtcgaggcgc cgacgaggtt gatcgccttt tgccactggc gcagcagctc
     5881 gagatagagc gtcaggcgct ccactgtttc acgtgaaaca ggcacctgcg ccgcgaagcc
     5941 cgccgcatcg agcggcgcct cacgccgcac gtcggacgtg ccggtagagg agcgtgaggg
     6001 ctgccggggt catgcccggc aggcgggcgg cggccccgag cgtggtcggc cggacgcggc
     6061 tcagcagcgc gcgcagctcg ccgctgagcc cggccagtgc ggcgaagtcg acctcgggcg
     6121 gcagatccag cgactcctcg cgacgaaaat ccgcgatgtc cgccacctga cgcttcagat
     6181 agggcgcata gcgctcaccg atctcgatct gctcggctat ctcggcggcg atgtcgccga
     6241 gctcgggcca gatggtcgcc agccgggcaa tatcgacttc cggcgaagcc aggagctgcc
     6301 gcgcggtgcg ggtcgccccg tcatggttca ccctgatgcc gtacgtcgca gcctccggcg
     6361 gtgtcagccg gtggtcatcg agcagcgcct cggcccgggt cagcttgtcc agcttgtcca
     6421 gaagtcgcgc atggcgcgcg gtgccgacga ggcccagccc ggtgccacgc tcggtcagcc
     6481 ggcgatcggc gttgtcggcg cggagcataa gccggtactc ggcgcggctc gtgaacatgc
     6541 gatagggctc ggtgatgccc tgtgtcgtga gatcgtcgac cagcacgccg atataggcct
     6601 ccgcccggtc gagcacggcg tccggccggc ggcctgatgc cctgagggcc gcgttggcgc
     6661 cggccatgat gccctgcgcc gccgcctcct cgtagccggt cgtgccgttg atctggccgg
     6721 cgaggaagag gccgggaagg gccctgagct cgagcgtcgg gtcgagctcc cgcggatcga
     6781 cgtgatcgta ttcgatggca tagccgggct gggtgatcac cgcccggccg agccccggaa
     6841 tggtccggac gagcgcctcc tgcacggccc gcggcagcga ggtggagatg ccgttggggt
     6901 agaccgtgtg gtcgtcgaga ccttccggct cgagaaagac ctgatggccg gtgcgctcgg
     6961 cgaagcgcac caccttgtcc tcgatcgacg ggcagtagcg cacgccggtg gccgcgatct
     7021 ggccggagaa gagcggcgcc tcgtgcagat gggcgcggat caggtcgtgg ctcgcctcgg
     7081 tcgtccgggt gacgtggcag gcgatctgcg ggttggtgat ccgcgtcgtg aggaacgaga
     7141 aggggcgggg cggctcgtcg ccgggctgcc gggccagcgc cgcccagtcg atggtgcgac
     7201 catcgagccg gggcggcgtg ccggtcttca gccggccgag ccggacaccc gctgcctcga
     7261 ggtcccgcgc cagggccacc gccggctggt cccccacccg cccggcgggc cagcgctcgg
     7321 tcccgagatg gatctcgccg cgcaggaacg tgccggtggt cagcaccact gccccggccc
     7381 gcagctcacg gccttcggcc gtcaccacgc cggtgaccct tgtgccatgg cgaagcaggc
     7441 ccgccgcctc gccctcggcg aggacgatcc cggcctgcgc ggccaggagg tcctggaccg
     7501 cccgggcata gagccggcgg tcggcctggg cgcgcgggcc ctgcacggct ggccccttgc
     7561 ggcggttgag gacgcggaac tggatgccgc ccgcatcgat ggcgcggccc atcaggccgt
     7621 cgagggcgtc gatctcgcgc accagatggc cgcgcccgag cccgccgatg gccgggttgc
     7681 aggacatcgc cccgatggtt gccagcttct gggtgagcag cagcgtgcgg gcacccatcc
     7741 gggcgctcgc cgccgccgct tcggcccccg catggccgcc gccgatgaca atgacgtcgt
     7801 ggctctccat ggccggctat gtgcgccggc gagccggcag cgtcaagcga ggcccggcga
     7861 cggctggacg gcggcggccg agacgtgctt tctgcggcgc atgagcggaa tcggtggcgt
     7921 catcctcggc ttcggcgtgc ttttggcact gatcgcacct ttgccgccat tggccgagcg
     7981 gctgcgcctg ccgtacaccg tcctgctcgc gattctgggc tgctgcctcg ggctgctgac
     8041 cgggtttgcc gcccggttcg aggagatgct catcgattcc gacgtgctcg acctggtggc
     8101 ggcgatcagc gagctcgagg tcggctcggc ggtcttcctc tgggtgttcc tcccgctgat
     8161 cctctttcac agtgccatgg agctcgacag tcgcgccctg ctcgaagata tcggcccggt
     8221 catcctcctc gcggtggtgg cggtccttgc gacgaccctg atcgccgggc tcgccatctg
     8281 gagcgtctcg accgtcacgc tcgcgagctg cctcctggtc ggggcgatca tcgccaccac
     8341 ggatccgatc gccgtcatca acgtctttca ggaagtcggc gcaccccggc ggctggtgag
     8401 cctcgtcgag ggcgagagcc tgctcaacga tgccgctgcc atcgccctca ccaccagcct
     8461 gctggcgctg gtggtcgggc tggctgcccc atcgttcgag acaatcgtcg gcgacttcgt
     8521 cgtcagcatg gccggcggag ccgtgttcgg cctcgtcgtc ggccggctgg ccgggctgct
     8581 gctgacccgg ctcgacgagg cggggccggc cgagatcacg ctcagcgtcg ccgtcgccta
     8641 tctcacctat gccattgccg agctgcagtt cggcatctcc ggcgtggtcg ccgtcgtggc
     8701 cgccggcatc gtcttcggtg cccagggccg cgccggcctg tcgcgggccg actggcgctc
     8761 gctcatcgcc atctgggcgc agctcagctt ctgggccagc tccctcatct tcgtcctggc
     8821 ggcgatgctg gccccgaaga cgctggcaac cgccggcccg ttcgatgcgg tgctgctggt
     8881 gctgctgacc ctgggggcgc tcggctcgcg ggccatgatc ctctacggcg ccttgccgct
     8941 gatggaggcc atcggcctcg ccggccgcat ccagaccagc ttcaagcatg tcatgctgtg
     9001 gggcgggctg cgcggcgcca tcaccctcgc cctcgccctc gcggtcaccg agaacccgct
//
LOCUS       DAOTFI010000199         9030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9202_length_9030_cov_7.238329, whole genome shotgun sequence.
ACCESSION   DAOTFI010000199 DAOTFI010000000
VERSION     DAOTFI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9202_length_9030_cov_7.238329"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="P5558_17855"
     CDS             complement(<1..395)
                     /locus_tag="P5558_17855"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRY26240.1"
                     /translation="MTDLDLDRRTGWPPELRVLLDRHPRESWPNAAGIGPTGRFWLQR
                     HDLFRELTQALTMAAGELQEDRVDAARFVPWFRPRIGFLLHELHGHHRIEDLHYFPVF
                     QAADPRLARGFEVLDADHHAIDRAIAELGT"
     gene            complement(419..1153)
                     /gene="mtgA"
                     /locus_tag="P5558_17860"
     CDS             complement(419..1153)
                     /gene="mtgA"
                     /locus_tag="P5558_17860"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRY26241.1"
                     /translation="MTSVARSRDRSGTAPKRRSLLARLLRRAGLWLFLLLCVLPPLVL
                     FAWRHLPPPATPLMLLRLLEGEGLDYRWRPMGRISPDLARAVIASEDNLFCEHLGFDL
                     TALRSEVERALAGERPRGASTLTQQTAKNLFLWPGRDLARKLLEAWLAPQLELILGKQ
                     RILELYLNIAELGPGIYGAEAASQAWFDKPASDLSPAEAARLAVILPAPRDRSPHGPY
                     ADDRGRVIQQRVGQLGPLLDCVGPTS"
     gene            complement(1150..1503)
                     /locus_tag="P5558_17865"
     CDS             complement(1150..1503)
                     /locus_tag="P5558_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26242.1"
                     /translation="MHRLLTSRAARGLLGLTLLGAVAQAGTARAGDLLLLQQLVPGNQ
                     SGTVFLMLDRGRISRATATRSGALEHTIEIRLATDPPETVTLRCNDEAITRQLLNTLR
                     TSAESMLDVTGRCRL"
     gene            1625..1753
                     /locus_tag="P5558_17870"
     CDS             1625..1753
                     /locus_tag="P5558_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26243.1"
                     /translation="MSSRNVIIVVAVIVVLAIIGWQLGWFGGAEVVEPAPPATTTN"
     gene            complement(1803..3212)
                     /gene="leuC"
                     /locus_tag="P5558_17875"
     CDS             complement(1803..3212)
                     /gene="leuC"
                     /locus_tag="P5558_17875"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRY26244.1"
                     /translation="MNGPQTLFDKIWQAHLVEEQDDGTCLIYIDRHLVHEVTSPQAFE
                     GLRTAGRRVRRPEATLAVVDHNVPTDPDRAKGIKDETARIQVEALEQNAREFGVELYD
                     MNDIRQGIVHIIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQT
                     LLQKRPRNMRITVDGELGFGVTAKDVILAIIGRIGTAGGTGHVIEYAGSVIRALSMEG
                     RMTVCNMSIEAGARAGLIAPDEKTFEYIKGRPMAPKAGAFEQAVAAWRLLPSDAGAFY
                     DKEVSLKAEDIEPQVTWGTSPQDVVGISGAVPDPTKVEDAGRRAAMERSLAYMGLEPG
                     TPMREVKVDRVFLGSCTNGRIEDLRAAAKVVEGKRIADTVRAMVVPGSGLVKHQAEAE
                     GLDLIFKAAGFEWREAGCSMCLGMNPDQLAPEERCASTSNRNFEGRQGRLGRTHLVSP
                     AMAVAAAITGRLTDVRELV"
     gene            complement(3265..3672)
                     /gene="rplS"
                     /locus_tag="P5558_17880"
     CDS             complement(3265..3672)
                     /gene="rplS"
                     /locus_tag="P5558_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRY26245.1"
                     /translation="MNVIQQIEQQLITSLTAERAVPEFRPGDTLRVNVRVIEGNRERV
                     QAFEGVCIARRNRGVNSSFTVRKISYGEGVERVFPLYSPRIDSVDVVRRGKVRRAKLY
                     YLRDRRGKSARIAERRTERPSSKAAAETTGSEA"
     gene            complement(3669..4409)
                     /gene="trmD"
                     /locus_tag="P5558_17885"
     CDS             complement(3669..4409)
                     /gene="trmD"
                     /locus_tag="P5558_17885"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRY26246.1"
                     /translation="MTTPWRASVLTIFPDLFPGPLGCSVLGRALEAGIWSLDAVDLRD
                     FATDRHRSVDDTPFGGGAGMVMRPDVVAAAVAAVHGPGDARPLLYMSPRGAPFSQDEA
                     RRLAGGPGAVILSGRYEGVDERAIEACGFLEVSLGDFVMTGGEIAAMALLDATVRLLP
                     GVVGSSASLEEESFSSGLLEYPHYTRPQSWEGRSVPPVLLSGHHAEIHAWRQAAALAL
                     TRRRRPDLLATAAGHPQPDQFAQPEPPR"
     gene            complement(4406..4945)
                     /gene="rimM"
                     /locus_tag="P5558_17890"
     CDS             complement(4406..4945)
                     /gene="rimM"
                     /locus_tag="P5558_17890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRY26247.1"
                     /translation="MSGSTQAPPNLVCVAAVAAAHGVRGALKLKSFTEQPENVAGYGA
                     LFDEEGHELFHVRAIGPCKGGVIVRAEGITDRDSAEALRGLRLYVPRDRLPATESDTF
                     YHADLIGLSVVTEAGEEVGRVVAVHDFGAGDVLEYVDAAGASQMVAFTLAHVPEVDLD
                     RRRVVVASAALPESAEAAA"
     gene            complement(4975..5316)
                     /gene="rpsP"
                     /locus_tag="P5558_17895"
     CDS             complement(4975..5316)
                     /gene="rpsP"
                     /locus_tag="P5558_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRY26248.1"
                     /translation="MAVKIRLARGGAKKRPYYRIIAADTRYARDGRFLEKLGHYNPLL
                     PDEHPERIVMNEERIRHYLGHGAQVSDRMARFLDKAGIVPGATRLRGTGKKADEIAAK
                     KAKAETEAASA"
     gene            complement(5374..6726)
                     /gene="ffh"
                     /locus_tag="P5558_17900"
     CDS             complement(5374..6726)
                     /gene="ffh"
                     /locus_tag="P5558_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRY26249.1"
                     /translation="MFDSLTKRLGGVFDRLRKRGALGEADVAEAMREIRIALLEADVA
                     LPVVKSFVAKVKERAVGQEVIKSVTPGQMVVKIVHDQLVELLGGSSAALELGGPPGIV
                     LMVGLQGSGKTTTSGKLALRLKDKDKKRVLLASLDVQRPAAQHQLEVLAGQAGVASVA
                     IVPGESPLAITRRALEQARREGYDTLILDTAGRLHIDDTLMDELAQVRDLARPHETLL
                     VADSLTGQDAVNVARHFAERIGITGIVLTRLDGDARGGAALSMREITGKPIKFVGTSE
                     KLAGLDAFHPERMASRILEMGDVVSLVEKASELVDKDEAEKLARKMTKGRFDLNDLQS
                     QLKQVGKMGGMGGILGMLPGVGKIKKQLAEANIDEKIILRQSAIIDSMTRLERRSPEI
                     IAASRKRRIAAGAGASVQEVNRLLKQHRQMHDMMKRMKKAGGPKQFLGRHMPPGMMPR
                     "
     gene            6967..7791
                     /gene="dapF"
                     /locus_tag="P5558_17905"
     CDS             6967..7791
                     /gene="dapF"
                     /locus_tag="P5558_17905"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRY26250.1"
                     /translation="MITFLKMHGLGNDFVVLDGRQGALPLDADLVRRLGDRHRGIGFD
                     QLALLTSSEIADVAVRFYNTDGSEAGACGNASRCIAALVGRELDRRQVTLATAGGVLP
                     SRLLDDGRIAVDMPPPRLAWQDVPLAGPADTLAVDLGVAGLPPAVALSMGNPHVVLFV
                     DDVDLAERHGAALERHALFPDRANIGFAQVLGPERIRLRVFERGAGLTLACGSGACAA
                     LVAAVRRGLVAERATMVLDGGELEVAWAGEGPVRMTGPYAISFSGSFDPATIGRRA"
     gene            7788..>9030
                     /gene="mtaB"
                     /locus_tag="P5558_17910"
     CDS             7788..>9030
                     /gene="mtaB"
                     /locus_tag="P5558_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRY26251.1"
                     /translation="MSDIDVVTFGCRLNIFESEVMRRHATAAGLDELVIVHTCAVTAE
                     AERQARQAIRRARRARPEARIVVTGCAAQITPDAYAGLAEVDHLVGNDTKLRPETWQG
                     LARPVPAVPAVGDIMALERTSGQMVEGFEQHTRAFVQVQNGCDHRCTFCVIPYGRGNS
                     RSVPAEDVLAQVRKLVEAGYKEVVLTGVDLTSYGRELDGRPPLGRLVGRILEEVPTLP
                     RLRLSSLDPAEIDDELFRHLTAEPRVMPHVHLSVQAGHDMILKRMKRRHLRHDVIELC
                     DRLRAARPGIVFGADLIAGFPTEDEAMFGQSLAIVEEAGLTFLHVFPYSPRPGTPASR
                     MPQLAKPLVKERAARLRAKGEAALDRFLRSEVGRRRRVLVERGDRGHTEEFAPIRLAG
                     PVPGGTLAEVAVTGVDGGLLVG"
BASE COUNT         1196 a         3093 c         3373 g         1368 t
ORIGIN      
        1 gtgccgagct cggcgatcgc ccggtcgatg gcgtgatggt cggcgtcgag cacctcgaag
       61 ccgcgggcga ggcgggggtc ggccgcctgg aagaccggga agtagtggag atcctcgatg
      121 cggtggtggc cgtgcagctc atgcaggagg aagccgatgc gcgggcgaaa ccagggaacg
      181 aagcgggcgg cgtcgacgcg gtcctcctgg agctcgccgg cggccatggt cagtgcctgg
      241 gtcagctcgc ggaagaggtc gtggcgctgg agccagaagc ggccggtcgg tccgattccg
      301 gcggcattcg gccaggactc gcgcgggtgg cggtcgagaa ggacgcgcag ctcgggcggc
      361 cagccggtgc ggcggtctag gtcgagatcg gtcatgcccg gctcctcatg acggcccctc
      421 atgacgttgg gccgacgcag tcgaggagcg ggccgagctg gccgacgcgc tgctggatga
      481 cgcggccgcg atcgtcggca taggggccat gcgggctgcg gtcacggggg gcggggagga
      541 tgacggcgag gcgggcggcc tcggcgggcg agaggtcgct ggcgggcttg tcgaaccagg
      601 cctgcgaggc ggcctccgca ccgtagatgc cggggccgag ctcggcgatg ttgaggtaga
      661 gctcgaggat gcgctgcttg cccaggatca gctcgagctg tggtgccagc caggcctcga
      721 gcagcttgcg ggccaggtcg cggccgggcc agaggaagag gttcttcgcc gtctgctggg
      781 tgagcgtgct ggcgccgcgc ggccgctcgc cggcgagggc ccgctcgacc tcggagcgga
      841 gtgccgtgag gtcgaagccc agatgctcgc agaagaggtt gtcctcggat gcgatcaccg
      901 cccgggcgag atcggggctg atccggccca tcggccgcca gcgataatcc agtccctcgc
      961 cttcgaggag cctgagcagc atgagcggtg tcgccggcgg cggcaggtgg cgccaggcga
     1021 agagcacgag cggcggcagg acgcagagga ggaggaagag ccagaggccg gcgcggcgca
     1081 gcagccgggc gagcaacgag cggcgcttcg gcgcggtgcc gctgcggtcg cgcgagcgcg
     1141 ccacggaggt cagagacggc aacggccggt gacgtcgagc atggattcgg cgctggtgcg
     1201 gagcgtgttg agcaactggc gggtgatcgc ctcgtcgttg cagcgcagtg tgacggtctc
     1261 gggcgggtcg gtggcgaggc ggatctcgat cgtgtgctcg agagcgccgc tgcgggtggc
     1321 cgtggcgcgg gagatgcggc cccggtcgag catcaggaag accgtgcccg actggttgcc
     1381 gggcacgagc tgctgcagga gcaggagatc gccggcccgg gcggtgccgg cctgggccac
     1441 ggcgcccagg agggtgaggc ccaggagtcc ccgtgcggcg cggctcgtca gcaatcggtg
     1501 catcggtcgt tcccccaggc cggcatcatg ctatatcttc gcccggctgc cagctacccg
     1561 gcgctcggca tcgcccctcg agatcgaacg cctgcaacag gaccggaaca agggaaatcg
     1621 cctcatgagc tcgcgcaacg tcatcatcgt cgtcgccgtc atcgtcgtgc tcgccatcat
     1681 cggctggcag ctcggctggt tcggcggtgc ggaggtggtc gagccggccc cgcccgcgac
     1741 caccaccaac tgagcggcgc gcgcgaccgg gccgcgcttg cggcccggtt gcgccggttc
     1801 cctcagacga gctcgcgcac gtccgtcagg cggccggtga tggctgccgc cacggccatg
     1861 gccgggctga cgaggtgggt gcggccgaga cggccctgcc ggccctcgaa attgcggttg
     1921 ctggtcgagg cgcagcgctc ctcgggggcg agctggtccg ggttcatgcc gaggcacatg
     1981 gagcagccgg cctcgcgcca ttcgaagccg gccgccttga agatcaggtc cagcccctcg
     2041 gcctcggcct ggtgcttcac gaggccggag cccggcacca ccatggcgcg cacggtatcg
     2101 gcgatgcgct tgccctccac caccttggcg gcggcccgaa gatcctcgat ccggccattg
     2161 gtgcatgagc cgaggaagac gcggtccacc ttgacctcgc gcatcggcgt gccgggctcg
     2221 aggcccatat aggcgagcga gcgctccatg gcggcccgcc ggccggcgtc ctccaccttc
     2281 gtcgggtcgg gcacggcgcc cgagatgccg accacgtcct gcgggctggt gccccaggtg
     2341 acctgcggct cgatgtcctc ggccttcagg ctcacctcct tgtcgtagaa ggcgcccgcg
     2401 tcggacggca gcagccgcca ggcggcgacc gcctgctcga aggcgcccgc cttgggcgcc
     2461 atcggacggc ccttgatgta ctcgaaggtc ttctcgtccg gggcgatcag gccggcccgc
     2521 gcacccgcct cgatcgacat gttgcagacc gtcatccggc cctccatcga gagcgcccgg
     2581 atcaccgatc cggcatattc gatgacgtgg ccggtgccgc ccgccgtgcc gatcctgccg
     2641 atgatggcga ggatcacgtc cttggcggtg acgccgaagc cgagctcgcc gtcgaccgtg
     2701 atccgcatgt tgcgggggcg cttctgcagc agcgtctgcg ttgccaggac gtgctcgacc
     2761 tcggacgtgc cgatgccgaa ggccagcgcc ccgaaggcgc cgtgcgtcga ggtgtggctg
     2821 tcgccgcaga cgatggtcgt gccgggcagg gtgaagccct gctccgggcc gatgatgtgc
     2881 acgatcccct ggcggatgtc gttcatgtcg tagagctcga cgccgaactc gcgggcgttc
     2941 tgctcgaggg cctcgacctg gatgcgcgcg gtctcgtcct tgatgccctt ggcccggtcc
     3001 gggtcggtcg gcacgttgtg gtcgaccacg gcgagcgtcg cctcgggccg gcgcacccgg
     3061 cggccggcgg tgcgcaggcc ctcgaaggcc tgcgggctgg tcacctcgtg gaccagatgc
     3121 cggtcgatgt agatcaggca ggtgccgtcg tcctgctcct cgacgagatg cgcctgccag
     3181 atcttgtcga agagggtctg cgggccgttc actgccatcg ggtcgattcc aggggctcga
     3241 ttccaggggg gcggcgaaag ccggtcaggc ttcgctgccg gtcgtctcgg cggcggcctt
     3301 cgaggacggc cgctcggtcc gccgctcggc gatgcgggcc gacttgccgc ggcggtcgcg
     3361 caggtaatag agcttcgcac gccgcacctt gccgcgccgc acgacgtcga cgctgtcgat
     3421 gcgcgggctg tagagtggga agacgcgctc cacaccttcg ccgtaggaga tcttgcggac
     3481 ggtgaaggac gagttcacgc cgcgattgcg cctggcgata cagacgccct cgaaggcctg
     3541 gacgcgctcg cggttgccct cgatcacgcg gacattgacc cgaagcgtgt cgcccgggcg
     3601 gaactcgggc accgcgcgct cggcggtcag ggatgtgatg agctgctgct cgatctgctg
     3661 gatgacgttc atcgcggggg ctccggctgg gcgaactgat cgggttgagg atggccggcg
     3721 gccgttgcca ggaggtccgg gcggcgtcgg cgggtcaggg cgagggcggc ggcttgccgc
     3781 caggcatgga tctcggcgtg gtggccggag aggaggacgg gcggcacgct ccgtccctcc
     3841 cagctctgcg gccgggtgta atgcgggtat tcgaggaggc ccgagctgaa gctctcctcc
     3901 tcgagcgagg cgctgctgcc gacgacgccg ggcaagaggc gcacggtcgc gtcgaggagc
     3961 gccatggcgg cgatctcgcc gccggtcatg acgaaatcgc cgagcgagac ttccagaaag
     4021 ccgcaggcct cgatcgcccg ctcgtcgacg ccctcgtagc ggcccgagag gatgacggcc
     4081 cctgggcccc ccgcgagccg gcgcgcctcg tcctggctga agggtgcgcc gcgcgggctc
     4141 atgtagagca gcgggcgcgc atcgccgggc ccgtgcaccg cggccaccgc cgccgccacc
     4201 acgtcgggcc gcatgaccat gccggccccg ccgccgaagg gcgtgtcgtc gacgctgcgg
     4261 tggcggtcgg tggcgaagtc gcgcagatcg accgcatcga gcgaccagat gccggcctcg
     4321 agcgcccggc cgagcaccga gcagccgagc gggcccggga agaggtcggg gaagatggtg
     4381 agcaccgagg cgcgccaggg ggtcgtcatg cggccgcctc cgcgctctcg ggcagggcgg
     4441 cgctcgccac caccacgcgg cgacggtcga gatcgacctc cggcacatgg gccagggtga
     4501 aggccaccat ctggctcgcc ccggccgcgt cgacatattc cagcacgtcg ccggcgccga
     4561 aatcgtgcac cgcgacgacc cggccgacct cctcgccggc ctcggtcacc acggagaggc
     4621 caatgagatc ggcatggtag aacgtgtcgc tctcggtcgc cggcaggcgg tcgcgcggca
     4681 cgtagagccg aaggccgcgc agcgcctcgg cgctgtcccg gtcggtgatg ccctcggccc
     4741 tgacgatgac cccgcccttg cacgggccga tggcccgcac gtggaacagc tcatggcctt
     4801 cttcgtcgaa gagcgcgccg tagccggcga cgttctccgg ctgctcggtg aagctcttga
     4861 gcttcagcgc accgcgcacg ccatgcgctg ccgcgaccgc ggcgacgcag acgaggttgg
     4921 ggggcgcttg ggtggagccg gacatgggct gggcgatccc gggccttggg cgcgtcaggc
     4981 gctcgcggcc tcggtctcgg ccttcgcctt cttcgcggcg atctcatcgg ccttcttgcc
     5041 ggtgccgcgc aggcgcgtcg cacccggcac gatgccggcc ttgtcgagga agcgggccat
     5101 gcggtcgctg acctgggcgc cgtggccgag atagtggcgg atgcgctcct cgttcatgac
     5161 gatgcgctcc ggatgctcgt cgggcagcag cgggttgtag tggccgagct tctcgaggaa
     5221 gcggccgtcg cgggcgtagc gcgtgtcggc ggcgatgatg cggtagtagg ggcgcttctt
     5281 cgcgccccct cgggccaggc ggatcttgac ggccatgaat gttccagacg gttggggttg
     5341 gttgaacggg gctcggtcgg ctcgagggcg gggctagcgg ggcatcatgc cgggtggcat
     5401 gtggcggccg aggaactgct tcggcccgcc cgccttcttc atccgcttca tcatgtcgtg
     5461 catctggcgg tgctgcttga gcagccggtt gacctcctgc accgaggcgc cggcgcccgc
     5521 ggcgatgcgc cgcttgcgcg aggccgcgat gatctcgggg ctgcggcgct cgaggcgggt
     5581 catgctgtcg atgatggccg actgccgcag gatgatcttc tcgtcgatat tggcctcggc
     5641 gagctgcttc ttgatcttgc cgacgccggg cagcatgccg aggatgccgc ccatgccgcc
     5701 catcttgccg acctgcttga gctggctctg caggtcgttg agatcgaagc ggcccttggt
     5761 catcttccgg gcgagcttct cggcctcgtc cttgtcgacc agctccgagg ccttctcgac
     5821 caggctgacg acgtcgccca tctcgaggat gcggctcgcc atgcgctcgg ggtggaaggc
     5881 gtcgaggccg gcgagcttct cgctggtgcc gacgaacttg atcggcttgc cggtgatctc
     5941 gcgcatgctg agcgcggcgc cgccccgcgc atcgccgtcg agtcgggtga ggacgatgcc
     6001 ggtgatgccg atgcgctcgg cgaaatggcg ggcgacgttg acagcgtcct ggccggtgag
     6061 gctgtcggcg acaagcagcg tctcgtgcgg ccgggcgagg tcgcgcacct gggccagctc
     6121 gtccatcagc gtgtcgtcga tgtgcagccg gccggcggtg tcgaggatca gcgtgtcgta
     6181 gccttcccgc cgcgcctgct cgagcgcccg gcgggtgatg gccagcgggc tctcgcccgg
     6241 cacgatggcc acgctcgcga cgccggcctg gccggccagc acctcgagct ggtgctgcgc
     6301 cgccgggcgc tgcacgtcga gcgaggcgag gaggacgcgc ttcttatcct tgtccttgag
     6361 gcgcagcgcc agcttgccgc tggtggtcgt cttgcccgag ccctgcaggc cgaccatgag
     6421 gacgatgccg ggcgggccgc cgagctcgag cgcggcgctc gagccgccca ggagctcgac
     6481 gagctggtcg tggacgatct tgacgaccat ctggcccggc gtgaccgact tgatcacctc
     6541 ctggccgacg gcgcgctcct tgaccttggc gacgaagctc ttgaccacgg gcagcgccac
     6601 gtcggcctcg agcagggcga tgcggatctc ccgcatggcc tccgccacat cggcctcgcc
     6661 gagcgcgccg cgcttgcgca gccgatcgaa cacgccgccc agacgcttgg tcaggctatc
     6721 gaacatgtcc cgcaccatgg acgaggaccc gggccggtcg cccgctgcgc cacgtcggtt
     6781 cctcgtgaag aagtcattgc gcaacagcgc tcgccgcgcc cttgcgggag cgtcgcgaag
     6841 gggctttcta ggcggccctg ccggccagcg tcaagtcgga acgggcggag ccggcggcgc
     6901 ggggcgacgc tttcacggcc cgacgggcaa ttgcgttcaa ggccgcttcg ccttatgccg
     6961 cagcgcatga tcaccttcct caagatgcac gggctcggca acgatttcgt cgtcctcgac
     7021 ggccggcagg gcgctttgcc gctcgacgcc gatctggtgc gccggctggg cgaccggcat
     7081 cgcgggatcg gcttcgacca gctcgccctg ctgacgtcga gcgagatcgc cgacgtggcc
     7141 gtgcgcttct acaacacgga cggctccgag gccggcgcct gcggcaatgc cagccgctgc
     7201 atcgccgccc tggtcggccg cgagctcgac cggcggcagg tgacgctggc gacggcgggc
     7261 ggcgtgctgc ccagccggct gctcgatgac gggcggatcg ccgtcgacat gccgcccccg
     7321 cgcctcgcct ggcaggacgt cccgctcgcc gggccggccg acacgctcgc cgtcgatctc
     7381 ggggtggccg gcctgccgcc ggcggtggcg ctctcgatgg gcaacccgca tgtggtgctg
     7441 tttgtcgacg acgtggacct ggccgagcgg cacggggccg ccctcgagcg gcatgcgctc
     7501 ttcccggacc gtgccaatat cggcttcgcg caggtcctgg ggccggagcg gatcaggctg
     7561 cgggtcttcg agcgcggtgc ggggctgacg ctcgcctgcg gcagcggcgc ctgcgccgcc
     7621 ctcgtggcgg ccgtccgccg ggggctcgtg gcggagcgcg ccaccatggt cctggacggc
     7681 ggcgagctgg aggtcgcctg ggccggggag ggcccggtcc ggatgaccgg tccctatgcc
     7741 atcagcttct ccggcagctt cgatcccgcc accatcgggc gccgggcatg agcgacatcg
     7801 acgtcgtcac cttcggctgc cgcctcaaca tcttcgaatc cgaggtgatg cgccgccacg
     7861 ccaccgcagc cgggctcgac gaactcgtca tcgtccacac ctgcgccgtc accgccgagg
     7921 ccgagcggca ggcgcgccag gcgatccgcc gggcgaggcg ggcacggccc gaagcacgga
     7981 tcgtggtcac cggctgtgcc gcgcagatca cgcccgacgc ctatgccggg ctcgccgagg
     8041 tcgaccacct tgtcggcaac gacacgaagc tccggcccga gacctggcag ggcctcgccc
     8101 ggccggtgcc cgcggtgccg gcggtcggcg acatcatggc gctggagcgg acctcgggcc
     8161 agatggtcga gggcttcgag cagcacaccc gcgccttcgt ccaggtgcag aacggctgcg
     8221 accatcgctg caccttctgc gtcattccct acggccgggg caacagccgg agcgtgccgg
     8281 cggaagacgt gctggcgcag gtccgaaagc tggttgaggc cggctacaag gaggtcgtgc
     8341 tgaccggcgt cgacctcacc tcctacgggc gcgagctgga cggccggccg ccgctcggcc
     8401 ggctggtcgg ccggatcctg gaagaggtgc cgacgctgcc gcggctgcgg ctctcctcgc
     8461 tcgacccggc cgagatcgac gacgagctct tccggcacct gacggcggag ccgcgggtca
     8521 tgccgcatgt gcacctcagc gtccaggccg gccatgacat gatcctcaag cggatgaagc
     8581 ggcggcatct gcgccacgac gtcatcgagc tctgcgaccg gcttcgcgcg gcccggcccg
     8641 gcatcgtctt cggcgccgac ctgatcgccg gctttccgac cgaggacgag gcgatgttcg
     8701 ggcagtcgct cgccatcgtc gaggaggccg gcctcacctt cctccacgtc tttccctatt
     8761 cgccccgccc cggcacgccg gcgtcccgca tgccgcaatt ggcgaagccg ctggtcaagg
     8821 aacgcgcggc gcggctgcgg gccaagggcg aggcggcgct cgaccgcttc ctcaggagtg
     8881 aggtcggccg ccgccgccgg gtgctggtcg agcgcggcga ccggggccac accgaggagt
     8941 tcgcccccat ccgcctggcc gggcccgtgc cgggcggcac cctcgccgag gtggcggtga
     9001 cgggggtcga tggcgggctg ctggtggggc
//
LOCUS       DAOTFI010000200         9025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9205_length_9025_cov_5.914270, whole genome shotgun sequence.
ACCESSION   DAOTFI010000200 DAOTFI010000000
VERSION     DAOTFI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9205_length_9025_cov_5.914270"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..576)
                     /locus_tag="P5558_17915"
     CDS             complement(289..576)
                     /locus_tag="P5558_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26252.1"
                     /translation="MSVTEAGAAGQTVSWRRLAMAVTFGLLALTVTACDDGPAEEAGS
                     EAGAAVDNAVDQAGDAAESAGDAIEDTAEDAADAVGDAAEDAGDAVEKATD"
     gene            787..1767
                     /locus_tag="P5558_17920"
     CDS             787..1767
                     /locus_tag="P5558_17920"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY26253.1"
                     /translation="MHIARAGAIAMALGIGGLVAGTAMAQSAKLGHLAPTDDPRHEVL
                     TAFAETVAAATGDAVTIEIFPNSTLGSERELFEQVQAGITEFALVGSIVANFYPPWSI
                     IDMPFMWRDSAHLQAFVTSEWAEQWSADMADELGVEMLAFLERNPRILTSATKPVHAV
                     EDLEGMKVRVPNINVYTDTWRAFGVEPVPMPAADFYLALRLGTIDAMENPVEVMYHWK
                     IYEVSKHLTLTNHMRAGFFLIASRQFMDGLEPAQQEAIRAAALEAQVLMAEKNAQGAA
                     ELFDKLRAEGMEIDENPDLSGFIAASEGVHETYMDQFGRDAYEAARAMGQ"
     gene            1790..2308
                     /locus_tag="P5558_17925"
     CDS             1790..2308
                     /locus_tag="P5558_17925"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRY26254.1"
                     /translation="MDRLITIGARLGALLRWLVAFAFAVMVAAAIAQVISRYVFNRPL
                     GWTEELARLVMLWWTFVAVGLLASSRKLMTLDALLYVLPGRGVHAVRAAAHLVAAVAA
                     LWLTWLGARLVLLAGRQVTPALDLPYGWVYLSLPVGLGIAGVAFVVHALVDLGHLRRD
                     TPVVRPGIGADI"
     gene            2308..3585
                     /locus_tag="P5558_17930"
     CDS             2308..3585
                     /locus_tag="P5558_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY26255.1"
                     /translation="MAWFAFLMVMLLGAVPIGFALVATGLGYLQVTGRVDALIAAQRV
                     ATGIDSFALMAIPFFLLAAELMNRTGITDRIFHFANCLLGRVAGGLGYVNVLASMMFA
                     SMSGSAVADAAGLGRIEIRAMTQAGYDRPFAAAITAASTVIAPIIPPSISLVIYGVTA
                     GASIGALFIAGLLPGILLGLALMVVVYIFARRRGYRAEKPASARETLCAAADAFLPLM
                     TPVIIVGGIWTGVFTPTEAGAFAVLYALLLGLVVYRSIGIGDLFQVLYESALSSANIL
                     FIIAVSALISWLLTVEQVPLRLAATIIDWSPHPIVFLLLLNGILLVLGCFMASAPIII
                     MLTPIVLPSAVAMGIDPVHLGVVMVLNLMIGLITPPIGLCLFAVSEVAGISPFALVRA
                     LLPFFVPLFFVLLLITFVPWTVLVLPKLLGFMG"
     gene            complement(3582..5054)
                     /locus_tag="P5558_17935"
     CDS             complement(3582..5054)
                     /locus_tag="P5558_17935"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRY26256.1"
                     /translation="MSAYVTLERRFKRLADLGGAAAVLNWDQAVMMPKGGNAARGEQL
                     AALGGLVHEILTAPQTGDLIEAAAAEPLDAWQGANLALVRRRFDRATALPQELVEATA
                     RATNACEMAWREARAASDYAALRPKLEEVLRLTREAASLQGAKLGLEPYPALLDGFQP
                     GLDEATIDRLFAPLQERLPGLIDDALARQGRALEPEGPFPVALQKQLAKELMARLGFD
                     FDHGRLDESTHPFCGGTPTDIRITTRYDEAEIVSAIMGVMHETGHALYEAGLPEPWRH
                     QPVGEAHGMAIHESQSLLIEMQACRSPAFLGFLAARLAETFGQQPALAPDNLIRHYRT
                     VQKGLIRVDADELTYPLHIVLRYRLEKALVTGDLDLHDLPAAWNDGMQELLGIRPPDD
                     RQGCLQDIHWPVGAFGYFPNYTLGALLAAQLFRAAEAAIPDLLAHIARGDFEPLLAWL
                     RTHVHEKASSLPLQGLIQEATGAELGTEAFLAHVGERYAR"
     gene            complement(5008..5988)
                     /locus_tag="P5558_17940"
     CDS             complement(5008..5988)
                     /locus_tag="P5558_17940"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRY26257.1"
                     /translation="MRALRPLALVSVVLTYCLIILGGWVRSTNSGLSCPDWPTCYGEW
                     VLTPGAFAALGEVGYTYGQVMLEWTHRLIAGVFLGPLILVLAVIAFFRRHADRAGWRL
                     ALGILLVLLIQAKLGGLTVLDANSPWSVALHLGNALLLLSLLVLFTLRLLPATAFAAP
                     EPAFWRPFLFCLWALVIATMITAAMTAKSGASLACYEWPACTGGWLPDLGDPQIRIHF
                     LHRVLAASTGLGILAFLVLAFRGDNPILRGLAAIVTLLVLAQIGLGALVIVHLVPTSL
                     AVAHQAVGVLLFTGIAAALATVLLTPTDAPTREPPHVGLRDPRAPLQASR"
     gene            complement(5985..6713)
                     /locus_tag="P5558_17945"
     CDS             complement(5985..6713)
                     /locus_tag="P5558_17945"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HRY26258.1"
                     /translation="MPDIRGRRFRPGWPLTLTTLAAFAVLMLLGTWQVQRLAWKLDLI
                     SRTEAGLAAAPVPLPADPAAMLALDLRPVTATGTLLHERAFAYGTSQRGGQPGARLMV
                     PLQRREGAPLLVDMGWIPEPIEAALDAPGQPDEVEITGILRADHQAARPNFRPENRPA
                     ERRWYWLDTAALASTLDLPELAPLTLGRSPSGAETTPPIADRPVLGLTNNHLGYAITW
                     YGLGLALVAVYLAFGLARGKEHNL"
     gene            complement(6719..7081)
                     /locus_tag="P5558_17950"
     CDS             complement(6719..7081)
                     /locus_tag="P5558_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRY26259.1"
                     /translation="MTGEPSPITTGLLGRCPACGRGRLFAGFLEVVPSCRCCGTALGG
                     QDSGDGPVAFIIMIAGGLVVGAALVVEVKHAWPLWLHMAVWLPLAVVLCLGLLRPLKG
                     LLIALQYRHRRTDFHADG"
     gene            complement(7098..7961)
                     /locus_tag="P5558_17955"
     CDS             complement(7098..7961)
                     /locus_tag="P5558_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRY26260.1"
                     /translation="MAEATHAGAHAAHAGPKHDYYLVDPSPWPLAGSGAALALTAGGV
                     MWMHGSGAGGYLALLGFAGVLATMFLWFRDVLKEAYAGDYSEKVASMLRIGMVLFIAS
                     EVLFFFAFFWAFFWGALVPPEIFPTSWPPEGIEPIPAFEIPFLNTMILLLSGCTVTWA
                     HHGLVHGDNETTKKALTLTVALGILFLSLQVYEYIHLVHLGMTIDKGIFGSAFYMATG
                     FHGFHVFVGATFLLVCTVRAYRGRLNKDRHVGFEAAAWYWHFVDVVWLFLFVCVYWWG
                     GSLFWTWPGIQ"
     gene            complement(7982..8572)
                     /locus_tag="P5558_17960"
     CDS             complement(7982..8572)
                     /locus_tag="P5558_17960"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRY26261.1"
                     /translation="MDARKNRRTALFGFGIILCMLGLTAASVPLYDLFCRVTGYGGTT
                     QTAETLPEIVSGPPIEVSFNADHEPGLPWSFRPAQRSVEVLPGENRLVFYEAANHADR
                     PIVGRAVYNVTPFKVGAYFAKIACFCFDEQTLQPGERVDMPVSFFVDPAILTDPETRD
                     VRQITLSYTFFIDEEATETLAAETAAHLHAPSDEPS"
     gene            complement(8576..8698)
                     /locus_tag="P5558_17965"
     CDS             complement(8576..8698)
                     /locus_tag="P5558_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26262.1"
                     /translation="MTKGDEQRRRLRAKNLAVAFLLLGLVVLIYWVAMVKMGGG"
     gene            complement(8695..>9025)
                     /locus_tag="P5558_17970"
     CDS             complement(8695..>9025)
                     /locus_tag="P5558_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRY26263.1"
                     /translation="LYRQKDYANVGVPMLPVVAGAATTRRHILGYSLLLAAISLLPSL
                     LGTAGWLYLATAALLSAGFVLGAWRLVRTASDRQAMRLFKFSIVYLFGLFLALVLDHV
                     AQPWLSA"
BASE COUNT         1343 a         2954 c         3376 g         1352 t
ORIGIN      
        1 gcatggaccg tggtgcggcc gggcaggccc tcctcgatga cgcggtagtc ggggccgagc
       61 tcggcggcga ggacaccggg ccagcgccgg gtggggccga ggcggcgaat gtcgcccggg
      121 ccggtcagcg gcagggtgcc gtgggtgttg gaatcgccga agcagagaat ggtgggcacg
      181 ggggtcgggt ccttcgggcg gcgggagaaa gagcggcgcg cctgtcgata gccccgatcg
      241 agggtgcggg acaagcggcg gctggccgcc tgccccgcag ccaggctcct agtccgtcgc
      301 cttctccacg gcgtcgccgg cgtcctcggc ggcgtcgccg accgcatcgg cggcgtcctc
      361 ggcggtgtcc tcgatggcgt cgccggcgct ttcggcggca tcgccggcct ggtcgacggc
      421 attgtccacg gctgccccgg cctcgctgcc ggcctcctcc gccgggccgt cgtcgcaggc
      481 cgtcacggtc agggccagga ggccgaaggt gacggccatc gccagtcggc gccaggacac
      541 cgtctgcccg gctgcgcccg cctcggttac cgacatgcca atcctccaaa ttgatcaaag
      601 acactacatg aacgatcagc cgcgactctt gttccgcccg ccggcgagat tcctcgatgc
      661 cgaggctgcc cctactacgt atgcgttgac gtattccttg ctcttcccta gcagatgggg
      721 atcaggggcg cgacgactcg ggcacccttg cggttcgagc aatctgggca atctgggagg
      781 atggacatgc acatcgcacg cgcaggcgcg atcgccatgg cgctcggcat cggcgggctg
      841 gtcgccggaa cggccatggc ccagtcggcc aagctcgggc atctcgcgcc gacggacgat
      901 ccgcgccacg aggtcctcac cgccttcgcc gagaccgtgg ccgcggcgac cggcgacgcc
      961 gtcaccatcg agatcttccc caactcgacg ctcggcagcg agcgcgagct cttcgagcag
     1021 gtccaggcgg gcatcacgga gtttgcgctc gtcggctcga tcgtcgccaa tttctacccg
     1081 ccctggtcga tcatcgacat gccgttcatg tggcgcgaca gcgcccatct ccaggccttc
     1141 gtcacgagcg aatgggccga gcagtggtcg gccgacatgg cggacgagct cggcgtcgag
     1201 atgctggcct tcctcgagcg caacccgcgc atcctgacct cggcgaccaa gccggtgcac
     1261 gcggtcgagg acctggaggg catgaaggtg cgggtgccca acatcaacgt ctacaccgac
     1321 acctggcgcg cgttcggggt cgagccggtg ccgatgccgg cggcggactt ctacctcgca
     1381 ctccggctcg gcacgatcga cgccatggag aacccggtgg aggtcatgta tcactggaag
     1441 atctacgagg tgtcgaagca cctgaccctg accaaccaca tgcgcgccgg cttcttcctc
     1501 attgccagcc ggcagttcat ggacggcctg gagccggcgc agcaggaggc gatccgtgcc
     1561 gccgccctgg aggcgcaggt gctgatggcc gaaaagaatg cccagggcgc tgccgagctt
     1621 ttcgacaagc tccgggccga gggcatggag atcgacgaga accccgatct ctccggcttc
     1681 atcgccgcct cggaaggcgt gcacgagacc tacatggacc agttcggccg tgacgcctac
     1741 gaggcggcgc gcgccatggg ccaataggcc ctcgaggagg gcacgggcga tggaccggct
     1801 gatcaccatc ggtgcgcggc tcggtgccct cctgcgctgg ctggtggcgt tcgccttcgc
     1861 cgtcatggtg gcggcggcga ttgcccaggt gatctcccgc tacgtcttca atcggcccct
     1921 gggctggacc gaggagctgg cgcggctggt catgctctgg tggaccttcg tcgccgtcgg
     1981 gctgctcgcc agctcccgca agctgatgac cctggatgcc ctgctctacg tcctgccggg
     2041 acggggcgtg catgccgtgc gcgcggcggc gcatctggtg gcggcggtgg ccgccctctg
     2101 gctgacctgg ctcggcgcaa ggctggtgct gctcgccgga cggcaggtga cgccggcgct
     2161 cgacctgccc tacggctggg tctatctcag cctgccggtc gggctcggca tcgccggcgt
     2221 cgccttcgtc gtccatgccc tggtcgatct cggccatctc aggcgcgaca cgcccgtggt
     2281 gcggcccggc atcggggccg acatctgatg gcctggttcg ccttcctcat ggtgatgctg
     2341 ctgggcgcgg tgccgatcgg cttcgccctg gtcgccacgg gtctcggcta cctccaggtg
     2401 acgggccgag tcgatgcgct gatcgcggca cagcgcgtgg ccacgggcat cgacagcttc
     2461 gcgctgatgg cgatcccctt cttcctgctc gccgccgagc tgatgaaccg gaccggcatc
     2521 accgaccgga tcttccactt cgccaactgc ctgctcggcc gggtggcggg cgggctcggc
     2581 tatgtcaacg tgctcgccag catgatgttc gcgtccatgt cgggctcggc cgtggccgac
     2641 gccgccgggc tcgggcggat cgagatccgg gcgatgaccc aggccggcta cgaccggccc
     2701 ttcgccgcgg ccatcaccgc agcctcgacg gtcatcgccc cgatcattcc gccctcgatc
     2761 tcgctggtca tctacggtgt gacggccggc gcctcgatcg gcgccctctt catcgccggc
     2821 ctcctccccg gcatcctgct cggcctggcg ctgatggtgg tcgtctacat cttcgcccgg
     2881 cgccgcggct accgggccga gaagccggcg agcgccagag agaccctctg cgccgcggcc
     2941 gatgccttcc tgccgctcat gaccccggtg atcatcgtcg gcggcatctg gaccggcgtc
     3001 ttcacgccga ccgaggcggg tgccttcgcc gtcctctacg cgctgctgct gggcctcgtc
     3061 gtctaccgct cgatcggcat cggcgacctc ttccaggtgc tctacgagag tgccctgtca
     3121 tcggccaaca tcctcttcat catcgccgtc tcggcgctga tatcctggct cctgacggtc
     3181 gagcaggtgc cgctcaggct ggccgcgacc atcatcgact ggtcgccgca cccgatcgtc
     3241 ttcctcctgc tgctcaacgg catcctcttg gtgctcggct gcttcatggc gtcggcgccg
     3301 atcatcatca tgctgacacc catcgtgctg ccctcggcgg tcgccatggg catcgatccg
     3361 gtgcatctgg gcgtggtcat ggtgctcaac ctgatgatcg ggctgatcac cccgccgatc
     3421 gggctctgcc tcttcgcggt gagcgaggtc gccggcatct cgcccttcgc cctggtgcgg
     3481 gcgctcctgc cctttttcgt gccgctcttt ttcgtgctgc tgctgatcac cttcgtgccc
     3541 tggaccgtgc tcgtcctgcc gaagctcctg ggcttcatgg gctagcgcgc gtagcgctcg
     3601 cccacatgcg cgaggaaggc ctcggtgccg agctcggcgc cggtggcttc ctggatcagc
     3661 ccctgcagcg gcaggctcga ggctttctcg tggacgtggg tgcgcagcca ggcgaggagg
     3721 ggctcgaagt cgcctcgggc gatgtgggcc aggaggtcgg ggatggcggc ttcggcggcc
     3781 cggaagagct gggcggcgag gagggcgccc agggtgtagt tgggaaagta gccgaaggcg
     3841 ccgaccggcc agtggatgtc ctgcaggcag ccctggcggt cgtcgggggg cctgatgccg
     3901 aggagctcct gcatgccgtc gttccacgcc gccgggaggt cgtggaggtc gaggtcgccg
     3961 gtgacgagcg ccttctcgag gcggtagcgg agcacgatgt ggagcgggta ggtgagctcg
     4021 tcggcgtcga cgcggatgag gcctttctgg accgtgcggt agtggcggat gaggttgtcg
     4081 ggcgccaggg ccggctgctg gccgaaggtt tcggcgaggc gggcggcgag gaagcccagg
     4141 aaggcgggcg agcggcaggc ctgcatctcg atcaggagcg actggctctc gtggatcgcc
     4201 atgccgtgcg cctcgccgac cggctggtgg cgccagggct cgggcaggcc ggcctcgtag
     4261 agggcgtggc cggtctcgtg catgaccccc atgatggccg agacgatctc ggcctcgtcg
     4321 tagcgggtgg tgatgcggat gtcggtgggg gtgccgccgc agaaggggtg cgtgctctcg
     4381 tcgagccggc catggtcgaa gtcgaagccg agcctcgcca tcagctcctt cgcgagctgc
     4441 ttctgcaggg ccacggggaa cgggccctcg ggctcgagcg ccctgccctg tcgcgcaagc
     4501 gcgtcgtcga tcaggccggg caggcgctcc tggagcgggg cgaagaggcg gtcgatggtg
     4561 gcctcgtcga ggcccggctg gaagccgtcg aggagggcgg gatagggctc gaggccgagc
     4621 ttcgcgccct gcagggatgc cgcctcgcgg gtcaggcgca ggacctcctc gagcttcggc
     4681 cgaagggcgg cgtagtcgct ggcggcccgg gcctcgcgcc aggccatctc gcaggcattg
     4741 gtggcccggg ccgtggcctc gaccagctcc tgcggcaggg cggtggcgcg gtcgaagcgg
     4801 cggcggacga gggcgagatt ggcgccctgc caggcatcga gcggctcggc cgcggcggcc
     4861 tcgatcaggt ccccggtctg cggtgccgtg aggatctcgt ggacgaggcc gcccagcgcc
     4921 gcgagctgct cgccgcgcgc ggcgttgccg cccttgggca tcatcaccgc ctggtcccag
     4981 ttgaggaccg cggcggcccc gccgaggtca gcgagacgct tgaagcggcg ctcgagggtc
     5041 acgtaggccg acatgcggcg gctccctggt tggcgcgtct gtgggggtga gcaggacggt
     5101 ggcgagagcc gccgcgatgc cggtgaagag gagcacgccc acggcctggt gcgccacggc
     5161 gaggctggtc gggacgagat ggacgatgac cagcgcaccg aggccgatct gggccagcac
     5221 caggagcgtg acgatggcgg cgaggccgcg caggatcggg ttgtcgcccc tgaaggccag
     5281 gacgaggaag gcgaggatgc cgagcccggt cgaggcggcg aggacgcggt ggaggaagtg
     5341 gatgcggatc tggggatcgc ccaggtccgg cagccagccg ccggtgcagg ccggccactc
     5401 gtagcaggcg agcgaggcgc cgcttttcgc ggtcatggcg gcggtgatca tggtggcgat
     5461 cacgagggcc cagaggcaga agaggaacgg ccgccagaag gccggctcgg gggcggcgaa
     5521 ggccgtggcc ggcaggaggc gcagcgtgaa gaggacgagg agcgagagca gcaggagcgc
     5581 gttgccgaga tgcagcgcca cggaccaggg gctgttggca tcgagcacgg tgaggccgcc
     5641 gagcttggcc tggatcaaga ggacgagcag gatgccgagg gcgaggcgcc agccggcccg
     5701 atcggcgtga cgcctgaaga acgcgatgac ggcgaggacg aggatcagcg gcccgaggaa
     5761 gacgccggcg atcagccggt gggtccattc gagcatgacc tggccgtagg tgtagccgac
     5821 ctcgccgagg gcggcgaagg cgccgggggt cagcacccac tcgccgtagc aggtcggcca
     5881 gtccgggcag gagagcccgg aattggtcga gcgcacccag ccgcccagga tgatcaggca
     5941 gtaggtcagg acgacgctga cgagcgccag cgggcgcagg gctctcataa attgtgctcc
     6001 ttgccgcggg cgaggccgaa ggcgagatag acggcgacga gcgccagtcc cagcccgtac
     6061 caggtgatgg cgtagcccag gtggttgttg gtcagcccca gcaccggccg gtcggcgatg
     6121 ggtggcgtgg tctcggcgcc ggacgggctg cggcccaggg tcagcggcgc cagttccggc
     6181 aggtcgagcg tgctcgcgag cgctgccgtg tcgagccagt accagcgccg ctcggccggg
     6241 cggttctcgg gtcggaaatt cggtctggcg gcctggtggt cggcgcgcag gatgccggtg
     6301 atctcgacct cgtcaggctg gccgggcgca tccagcgccg cctcgatggg ctcgggaatc
     6361 cagcccatgt cgacgaggag cggtgcgccc tcgcggcgct gcagcggcac catcaggcgg
     6421 gcgccgggct ggccgccccg ctggctggtg ccgtaggcga aggcgcgctc gtgcagcagc
     6481 gtgccggtgg cggtcaccgg cctgaggtcg agggcgagca tggcggcggg gtcggccggc
     6541 aagggcacgg gcgcggcggc gaggcccgcc tcggtccggc tgatgagatc gagcttccag
     6601 gcgaggcgct ggacctgcca ggtgccgagc agcatgagca cggcgaaggc cgcgagggtg
     6661 gtcagcgtga gcggccagcc gggccgaaag cggcggcctc tgatatccgg catgcggctc
     6721 aaccgtcggc gtggaagtcg gtccggcgat gccggtactg aagcgcgatc agcaggccct
     6781 tgaggggtcg cagcaggccg aggcagagga cgacggcgag cggcagccag accgccatgt
     6841 gcagccagag cggccaggca tgcttcacct cgacgacgag ggcggcgccg acgacgagcc
     6901 cgcctgcgat catgatgatg aaggcgaccg ggccgtcgcc gctgtcctgg ccgccgagcg
     6961 ccgtgccgca gcaccggcag ctcggcacga cctcgaggaa gccggcgaac aggcggcccc
     7021 ggccgcaggc cgggcagcgg ccgaggagac cggtcgtgat cggcgacggc tcgcccgtca
     7081 cccgttcacc gggacgctca ctggatgccg ggccaggtcc agaacaggct gccgccccac
     7141 cagtagacgc agacgaagag gaagagccag acgacgtcga cgaaatgcca gtaccaggcg
     7201 gccgcctcga agccgacatg ccggtccttg ttcagccggc cgcgataggc gcgcaccgtg
     7261 cagacgagca ggaaggtggc gccgacgaag acgtggaagc cgtggaagcc ggtggccatg
     7321 tagaaggccg agccgaagat ccccttgtcg atggtcatgc cgagatgcac gaggtggatg
     7381 tactcgtaga cctgcaggct gaggaagagg atgccgagcg ccacggtcag cgtcagcgcc
     7441 ttcttggtcg tctcgttgtc gccgtgcacc aggccgtgat gcgcccaggt gaccgtgcag
     7501 ccggagagca gcaggatcat cgtgttgagg aaggggatct cgaaggccgg gatgggctcg
     7561 atcccttccg gtggccagga cgttgggaat atctccggcg ggaccagcgc cccccagaag
     7621 aaggcccaga agaaggcgaa gaagaagagc acctccgacg cgatgaagag caccatgccg
     7681 atgcgcagca tcgaggcgac cttctcgctg tagtcgccgg cataggcctc cttcagcacg
     7741 tcgcggaacc agaggaacat ggtggcgagg acgccggcga agccgagcag cgccaggtag
     7801 ccgccggcgc ccgagccgtg catccacatc acgccgccgg cggtgagcgc cagggcggcg
     7861 ccgctgccgg cgagcggcca ggggctcggg tcgacgaggt agtagtcgtg cttcggcccg
     7921 gcgtgcgccg cgtgggcacc ggcgtgagtg gcttcggcca tggtcggatg gtcccccgtt
     7981 gtcagctagg ctcgtcgctc ggcgcatgga ggtgcgccgc ggtctcggcc gcgagcgtct
     8041 cggtcgcctc ttcgtcgatg aagaacgtat aagaaagggt gatctggcgc acgtcccgcg
     8101 tctccggatc cgtcaggatc gccgggtcga cgaagaacga gaccggcatg tcgacgcgct
     8161 cgcccggctg cagggtctgc tcgtcgaagc agaagcaggc gatcttggcg aaataggcgc
     8221 cgaccttgaa gggcgtcaca ttgtagacgg cgcggccgac gatcggccgg tcggcgtggt
     8281 tggcggcctc gtagaagacg agccggttct cgccgggcag cacctcgacc gagcgctggg
     8341 ccggccgaaa ggaccagggc agccccggct cgtgatcggc gttgaagctc acctcgatgg
     8401 gcggtcccga gacgatctcg ggcagcgtct cggcggtctg ggtggtgccg ccatagccgg
     8461 tgacccggca gaagaggtcg tagagcggca ccgacgccgc ggtcagcccc agcatgcaga
     8521 ggatgatgcc gaagccgaag agtgcggtgc ggcggttctt ccgcgcgtcc atcgctcagc
     8581 ccccgcccat cttcaccatg gcgacccagt agatgagcac gaccagcccc aggaggagga
     8641 aggccaccgc gaggttcttg gcgcgcaggc ggcggcgctg ctcgtcaccc ttggtcatgc
     8701 gctcagccag ggctgggcca cgtggtcgag gaccagggcc aggaagaggc cgaagaggta
     8761 gacgatcgag aacttgaaga ggcgcatcgc ctggcggtca ctggcggtcc gcacgagccg
     8821 ccaggcgccg agcacgaacc cggcggagag gagggcggcg gtggcgaggt agagccagcc
     8881 ggcggtgccg aggaggctcg gcagcaggct gatggcggcg agcaggaggc tgtagcccag
     8941 gatgtgccgg cgggtcgtgg cggccccggc gacgaccggc agcatcggca cgccgacatt
     9001 ggcgtagtcc ttctgccggt agagg
//
LOCUS       DAOTFI010000201         8996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9255_length_8996_cov_7.617045, whole genome shotgun sequence.
ACCESSION   DAOTFI010000201 DAOTFI010000000
VERSION     DAOTFI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9255_length_8996_cov_7.617045"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="P5558_17975"
     CDS             <1..787
                     /locus_tag="P5558_17975"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRY26264.1"
                     /translation="VVYEQRLELMQAEDVSETIADMRHEVLDDLVAKHIPEKSYAEQW
                     NGAGLAADVKRVLAIDAPVEEWLKEEGIAETEVRERLDALVEKKVAQKVANYGESMIR
                     MAEKSLLLQILDHLWKEHLLQLDHLRQGIHLRGYGQRDPLNEYKREAFALFETMLGRL
                     RETVTQVLAHLEIRMQPPAGGGQAGVAAAGATVPPAGGAMRQPLPPQSAQPAQPNGDG
                     GGVATAERPRTVAAEGDWGKVPRNAPCPCGSGKKFKQCHGQIT"
     gene            complement(831..1751)
                     /locus_tag="P5558_17980"
     CDS             complement(831..1751)
                     /locus_tag="P5558_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRY26265.1"
                     /translation="MKTIASVGECMIELSEAGQGLMRRGFGGDTLNTAVYLRRELAPA
                     EARVAYVTGLGDDPYSDELLAGWQAEGVDTSEVARLQGRLPGLYAIRTDANGERSFAY
                     WRSEAAARSVFAGSEGSARLERLGNYDLVYFSGISLAILPAADRDRLLVRLAALRDGG
                     RQIAFDPNFRPRLWPDLDEARATMTRAAGLATIVLPSFADEQTLFGDSEPGATLDRLH
                     ALGALEVIVKDGEAPMHFRVDGESFEALPVRVADPVDTTGAGDSFNAGYLAARLRGAT
                     MPQAVDAGSRLAARVVRHRGAIIPKDAPLY"
     gene            1913..2101
                     /locus_tag="P5558_17985"
     CDS             1913..2101
                     /locus_tag="P5558_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HRY26266.1"
                     /translation="MTPLHYLLFAAMLATLGVLIVGVTGFLRGGAFNEKYGNKLMRAR
                     VGLQFVALAILAILFLAH"
     gene            2136..2705
                     /locus_tag="P5558_17990"
     CDS             2136..2705
                     /locus_tag="P5558_17990"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRY26267.1"
                     /translation="MVLLTKIYTRGGDRGQTSLGDGARVAKHDLRVQAYGTVDEVNAT
                     VGLVRLHAGGDAGAEAMLRRIQNDLFDLGADLCRPGADAADATTLRIQESQVLRLEQE
                     IDAMNEALAPLTSFVLPGGTPAAAHLHLARTVCRRAERLVSALLEQEPVNALALRYLN
                     RLSDHFFVMSRHLNDKGRLDVLWQPGANR"
     gene            2853..3602
                     /locus_tag="P5558_17995"
     CDS             2853..3602
                     /locus_tag="P5558_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRY26268.1"
                     /translation="MKVLVPVKRVVDYNVKIRVKSDQSGVETANVKMSMNPFDEIANE
                     EAIRLREKGIAEEVVAVSLGEQKCQETLRTALAMGADRAIHVPADASLQPLAVAKCLK
                     VIAEREAPGLILLGKQAIDDDSNQTGQMLAALLGWAQATFVSKLEVKDGKAICGREVD
                     EGIETIEVKLPAIITVDLRLNEPRYASLPNIMKAKKKPIDTITAEELGVDVAPRLVYT
                     KFEEPPKRQAGVRVADVAELVAKLKTEAGVI"
     gene            3602..4546
                     /locus_tag="P5558_18000"
     CDS             3602..4546
                     /locus_tag="P5558_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY26269.1"
                     /translation="MAVLILAQHDNQTLDPAVLHAVTAAAALPGPMHVLVAGAECGAV
                     AEAAAKIAGIEKVIRVEHSAYANPVAEDLTPLVVAMAADYDYVLAASTTFSRNLLPRV
                     AALLDTAQLSDIVEVVSPSTFRRYVYAGNALTTVESKDEKRVITVRTTTFEAAPAEGG
                     SAEITTVAAVDPAGLARFVGREVQSSARPDLTSARVVVSGGRGIGSAENFSIVEALAD
                     TLGAAVGASRAAVDAGYAPNDWQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEEAPIFQVADYAIVGDLFKLVPELTEALRQAKG"
     gene            4572..5483
                     /locus_tag="P5558_18005"
     CDS             4572..5483
                     /locus_tag="P5558_18005"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRY26270.1"
                     /translation="MNVYTEKDYPPLPEVQRIGVIGAGQMGGGIAQVAAQAGIDVVLV
                     DVRREQLEAAMELMGRLLDRQVQKGTIEAADRDAALNRVSTSTDMADLGGCDLVVEAA
                     TENEELKKKIFKGLVPHLKADAIVASNTSSISITRLAAATDRPERFIGMHFMNPVPAM
                     KLVEVIRGIATAEDTYRSIRGLCERLGKTPVTAEDFPAFIVNRILLPMINEAVYTLYE
                     GVGTVESIDTAMKLGANHPMGPLELADFIGLDTCLAVMQVLYDGLADSKYRPCPLMVK
                     YVEAGWLGRKVKRGFYDYRGETPVPTR"
     gene            complement(5552..6214)
                     /locus_tag="P5558_18010"
     CDS             complement(5552..6214)
                     /locus_tag="P5558_18010"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRY26271.1"
                     /translation="MTLAIGATAPDFTAETTEGRINFHEWAGDSWVVLFSHPKDFTPV
                     CTTELGTMARIKPEFDKRGVKIIGLSVDPVDDHVRWSKDIEETMGAAPNYPMIGDQDL
                     AVAKLYGMLPEGAGDTAKGRTAADNATVRNVYVVGPDKKVKLVLSYPMSTGRNFDEIL
                     RIVDSLQLTARHQVATPANWNQGDDVIIVPSVSDEAAKAKFPDGWKALKPYMRVVAQP
                     KG"
     gene            6361..7296
                     /locus_tag="P5558_18015"
     CDS             6361..7296
                     /locus_tag="P5558_18015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HRY26272.1"
                     /translation="MPKRIVTLTLNPAVDASCATEEVRPVNKLRTTNDRLDPGGGGIN
                     VARVVNELGGQSFAVYAAGGRSGDVLDELVRDAGVMAHRIATAEPTRVSHVVFERRSG
                     QEYRFTPEGPMLAESEWQAALAFVELLDFDYLVASGSLPRGVPDDLYARLAEVVKKRR
                     ARLVVDTSGPALAAALGAGVHLVKPNRSEFARLLGRDLGDPAELAAAATELVASGSTE
                     LLAVTMGHEGALLATPDGIWRLGTPEVEARSAVGAGDSFVAGMTWGLASGESVERAFA
                     LGVAAGTATVLTPGTELCHLVDVQRFFRDLRPVRA"
     gene            7355..7774
                     /locus_tag="P5558_18020"
     CDS             7355..7774
                     /locus_tag="P5558_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRY26273.1"
                     /translation="MVETPAERSPRTPDSEKITLNLGYVDLGRIDLLVQEGFYANRSD
                     FIRTAIRNQLDRHAEAVDRSVGRQGLDLGLRRYSRADLEAMRAAGEMLHINVLGLATI
                     ASDVTPELARATIASVTVLGALNATPAVKAALQDRMH"
     gene            7803..8843
                     /locus_tag="P5558_18025"
     CDS             7803..8843
                     /locus_tag="P5558_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRY26274.1"
                     /translation="MNMIDSGRMAEATRLTRAGRLAEATALLQGALGGAATAPPADGW
                     QGPTLDLRAEPLPEASPGGEQWLARSHANPFGRRDYRLFVPAAAANEQRPLLVMLHGC
                     TQNPDDFAAGTAMNRLAAGAGLIVAWPGQCQTANPQRCWNWFQPTDQERARGEPSIIA
                     GITREVMRDHSVDAGRVYVAGLSAGGAEAAVLAATYPDVYAAFGVHSGLAHGAARDVA
                     SAFQAMREPAAGRPASVGGRKMPVIVVHGDRDATVHPGNGAAVIRQAVGTLETTVERG
                     RVPNGHAWTRTRHLDQAGQTLAEHWQVHGLGHAWSGGNAAGSYTDPRGPDASRAMLDF
                     FLGHRNGPAAAG"
BASE COUNT         1392 a         3246 c         3059 g         1299 t
ORIGIN      
        1 ggtcgtctac gagcagcggc tcgagctgat gcaggccgag gacgtctcgg agaccatcgc
       61 cgacatgcgc cacgaggtgc tcgacgacct ggtagccaag cacatccccg agaagtccta
      121 cgccgagcag tggaacggcg ccggcctcgc cgccgacgtc aagcgcgtgc tggcgatcga
      181 cgcacctgtc gaggagtggc tgaaggagga gggcatcgcc gagaccgagg tgcgcgagcg
      241 gctcgatgcc ctggtcgaga agaaggtcgc ccagaaggtc gccaactacg gcgaatcgat
      301 gatccgcatg gccgagaaga gcctgctcct gcagattctc gaccatctgt ggaaggagca
      361 tctgctgcag ctcgaccact tgcggcaggg catccatctg cgcggctacg gccagcgcga
      421 ccccctgaac gagtacaagc gcgaggcctt cgccctcttc gagaccatgc tcggccggtt
      481 gcgcgagacg gtcacccagg ttctggcgca tctcgagatc cgcatgcagc cgccggccgg
      541 cggcggtcag gcaggtgtgg cggccgctgg cgcgaccgtg ccgccggccg gcggcgcgat
      601 gcggcagccc ctgccaccgc agtcggccca accggcccag ccgaatggcg acggcggcgg
      661 ggtcgccacc gccgagcggc ctcggaccgt ggcggccgag ggcgactggg gcaaggtgcc
      721 gcgcaacgcc ccctgcccct gcggctccgg caagaagttc aagcagtgcc acggccagat
      781 cacctgatcg gcccgctgcc acatcgcccg acggtcagcg cggcaatgct ctagtagagc
      841 ggtgcatcct tcgggatgat ggcgccgcga tggcgcacga cccgggccgc gagccgcgag
      901 ccggcatcca cggcctgcgg catcgtcgcc ccgcgcagcc gggccgcgag gtagccggcg
      961 ttgaaggaat cgccggcgcc cgtggtatcg acgggatccg cgacacgcac cggcagcgcc
     1021 tcgaagctct cgccatcgac gcggaaatgc atgggcgcct cgccgtcctt gacgatcacc
     1081 tccagggcac ccagcgcatg cagccgatcg agggtcgccc cgggctcgga atcgccgaac
     1141 agcgtctgct cgtcggcgaa gctcggcagc acgatcgtgg cgaggccggc ggcccgggtc
     1201 atcgtcgccc gcgcctcgtc gaggtcgggc cagagccgcg gccggaaatt ggggtcgaag
     1261 gcgatctgcc ttccgccatc ccgcaaggcg gcgagccgca ccagcagccg gtcgcgatcc
     1321 gcggccggca ggatggcgag gctgatgccc gagaaataga cgaggtcgta attgccgagc
     1381 cgctcgaggc gcgcgctgcc ctcgctgccg gcgaagaccg agcgggcggc cgcctcgctg
     1441 cgccagtagg cgaagctgcg ctcgccattg gcatccgtgc ggatggcgta gaggccgggc
     1501 agccggccct gcagccgggc aacctcgctg gtgtcgacgc cctccgcctg ccagccggcc
     1561 aggagctcgt cgctgtaagg gtcgtcgccc aggcccgtga cataggcgac gcgggcctcg
     1621 gccggcgcca gctcgcggcg gagatagacg gcggtgttca gcgtgtcgcc gccgaagccc
     1681 cggcgcatca ggccctggcc ggcctcgctg agctcgatca tgcactcgcc gacgctggcg
     1741 atggtcttca cgctggccct ccctcgaact agttgaggca tgcccgagcg cgtcgtcgcc
     1801 ggcaagtatg gaggccgggg cttgcgcgcg ggcgcctgga atgatcttgt tgcgccgggc
     1861 tggcccggtg gctggcccga tcaccatcgt atagcatcgc atcggttgtc acatgacccc
     1921 gctccactat ctcctcttcg ccgccatgct cgcgacccta ggtgtgctga tcgtcggtgt
     1981 gaccggcttt ctcaggggcg gggccttcaa cgagaaatac ggcaacaagc tgatgcgcgc
     2041 ccgggtcggc ctgcagttcg tggcgctggc gatcctggcc atcctgttcc tcgcgcattg
     2101 acaccccgag cacccacctc agccggaacc tggtcatggt cctcctgacg aagatctaca
     2161 cgcgcggcgg cgaccgcggg cagacgtcgc tcggcgacgg tgcccgggtc gccaagcacg
     2221 acctgcgcgt gcaggcctac ggcacggtcg acgaggtcaa cgccacggtc gggctggtgc
     2281 gcctgcatgc cggcggcgac gccggggccg aggcgatgct gcgccggatc cagaacgatc
     2341 tcttcgacct cggcgccgat ctctgccggc ccggcgcgga tgccgccgat gccacgacgc
     2401 tgcgcatcca ggagagccag gtcctgcggc tcgagcagga gatcgacgcc atgaacgagg
     2461 cgctggcgcc gctcacctcg ttcgtgctgc cgggcggcac gccggccgcc gcccatctcc
     2521 acctcgcccg cacggtctgc cgccgggccg agcggctggt gagcgcgctg ctcgagcagg
     2581 agcccgtcaa tgccctggcg ctgcgctacc tcaaccggct ctccgaccac ttcttcgtca
     2641 tgtcccgcca cctcaacgac aaggggcggc tcgacgtgct ctggcagccc ggggcgaacc
     2701 gctgaccaca cctgggggcc gggtgttggt gccgggcgag gtgttggttc gttgacaagg
     2761 cgccggccgg cgcatatctg tccctattgc agtgcacaat aaacgggccg cgcgatcgaa
     2821 cactgcgcga tcgttgagca gaggttgcgg acatgaaggt gctcgtgccc gtcaagcggg
     2881 tggtcgacta caacgtcaag atcagggtca agtccgacca gagcggggtc gagacggcca
     2941 acgtcaagat gtcgatgaac ccgttcgacg agatcgccaa cgaggaggcg atccgcctgc
     3001 gcgagaaggg catcgccgag gaggtggtcg cggtctcgct gggcgagcag aaatgccagg
     3061 agaccctgcg caccgcactc gccatggggg cggaccgggc gatccacgtg cccgccgacg
     3121 cgtcgctgca gccgctggcc gtcgccaagt gcctgaaggt gatcgccgag cgcgaggcgc
     3181 cggggctcat cctcctcggc aagcaggcga tcgacgacga cagcaaccag accggccaga
     3241 tgctcgcagc attgctcggc tgggcccagg cgaccttcgt ctcgaagctc gaggtcaagg
     3301 acggcaaggc gatctgcggc cgcgaggtgg acgagggcat cgagacgatc gaggtgaagc
     3361 tgccggcgat catcacggtg gacctgcgcc tcaacgagcc gcgctacgcc tccctgccca
     3421 acatcatgaa ggcgaagaag aagccgatcg acacgatcac cgccgaggag ctcggcgtcg
     3481 atgtcgcccc ccggctggtc tacacgaagt tcgaggagcc gccgaagcgg caggccggcg
     3541 tccgggtcgc cgatgtggcg gagctggtcg ccaagctgaa gaccgaggcg ggagtgatct
     3601 gatggccgtt ctgatcctcg cccagcacga caaccagacc ctcgaccccg cggtgctgca
     3661 cgcggtgacc gccgccgctg ccctgcccgg ccccatgcac gtgctggtcg ccggcgccga
     3721 gtgcggtgcc gtggccgagg ccgcagccaa gatcgccggc atcgagaagg tgatccgggt
     3781 cgagcacagc gcctatgcca accccgtggc cgaggacctg acgccgctcg tcgtcgccat
     3841 ggcggcggac tacgactacg tcctcgccgc ctcgaccacc ttcagccgca acctcctgcc
     3901 gcgcgtggca gcattgctcg acacggccca gctcagcgac atcgtcgagg tcgtctcgcc
     3961 ctcgaccttc cgccgctacg tctatgccgg caacgcgctc accacggtcg agagcaagga
     4021 cgagaaacgg gtcatcacgg tgcgcaccac caccttcgag gcagcaccgg ccgagggcgg
     4081 cagcgcggag atcaccaccg tggcggcggt cgacccggcc ggcctcgccc gtttcgtcgg
     4141 ccgcgaggtg cagtcgagcg ccaggcccga cctcacctcc gcccgggtcg tcgtctccgg
     4201 cggtcgcggc atcggctcgg ccgagaactt ctccatcgtc gaggcgctgg ccgacacgct
     4261 gggtgctgcc gtcggcgcca gccgcgccgc cgtcgatgcc ggctacgccc ccaatgactg
     4321 gcaggtcggc cagaccggca agatcgtggc gccggacctc tacatcgccg tcggcatctc
     4381 cggtgcgatc cagcatctcg ccggcatgaa ggacagcaag gtcatcgtcg ccatcaacaa
     4441 ggacgaggaa gcgccgatct tccaggtcgc ggactacgcg atcgtcggcg acctcttcaa
     4501 gctcgtgccc gagctcaccg aggcgctgcg gcaggccaag ggttgagcgg cgtcgcccca
     4561 ggagcgtctg aatgaacgtc tacaccgaga aggactaccc gcctctgccc gaggtccagc
     4621 ggatcggcgt gatcggcgcc ggccagatgg gcggcggcat cgcccaggtc gcggcccagg
     4681 cgggcatcga cgtggtgctc gtggacgtcc gacgcgagca gctcgaggcg gccatggagc
     4741 tgatgggccg gctgctcgac cggcaggtgc agaagggcac gatcgaggcc gccgaccgtg
     4801 acgcggcgct caaccgcgtg tcgaccagca cggatatggc cgatctcggc ggttgcgacc
     4861 tcgtggtcga ggccgccacc gagaacgagg agctgaagaa gaagatcttc aaggggctgg
     4921 tgccgcatct caaggcggac gcgatcgtcg cctccaacac ctcgtcgatc tcgatcaccc
     4981 gcctcgccgc cgccactgac cggcccgagc gcttcatcgg catgcacttc atgaacccgg
     5041 tgccggcgat gaagctggtc gaggtgatcc gcggcatcgc cacggccgag gacacctacc
     5101 gctccatccg cggcctgtgc gagcggctcg gcaagacgcc ggtcaccgcc gaggacttcc
     5161 cggccttcat cgtcaaccgc atcctgctgc cgatgatcaa cgaggcggtc tacacgctct
     5221 acgagggtgt cggcacggtc gagagcatcg acaccgccat gaagctcggc gccaaccacc
     5281 cgatgggccc cctcgagctc gccgacttca tcggcctcga tacctgcctc gccgtcatgc
     5341 aggtgctcta tgacgggctc gccgacagca agtaccgtcc ctgcccgctc atggtgaaat
     5401 atgtcgaggc cggctggctc ggccgcaagg tcaagcgcgg cttctacgac taccgcggcg
     5461 agacgcccgt cccgacccgc tgaggcgccc gcgctttggc gcaacgcacc gtggcccgtg
     5521 gctggacgga ccacggtgct cggggccgcg ttcagccctt gggctgcgcc accacccgca
     5581 tgtagggctt gagggccttc cagccgtccg ggaacttggc cttggccgcc tcgtcggaca
     5641 ccgagggcac gatgatgacg tcgtcgccct ggttccagtt ggccggcgtc gccacctggt
     5701 gcctggcggt gagctgcaat gaatcgacga tgcgcaggat ctcgtcgaag ttgcggccgg
     5761 tgctcatcgg ataggagagc acgagcttca ccttcttgtc cggccccacg acatagacgt
     5821 tccgaacggt cgcgttgtcg gcggcggtgc ggcccttggc ggtgtcgccg gcgccctcgg
     5881 gcagcatgcc gtagagcttc gccacggcga ggtcctgatc gccgatcatc gggtagttgg
     5941 gggccgcgcc catcgtctcc tcgatgtcct tcgaccagcg gacatggtcg tccacggggt
     6001 cgacgctgag gccgatgatc ttgacgccgc gcttgtcgaa ctcgggcttg atcctcgcca
     6061 tggtgccgag ctcggtggtg cagaccgggg tgaagtcctt gggatgcgag aagagcacga
     6121 cccagctgtc gccggcccac tcgtggaaat tgatgcgtcc ctcggtggtc tcggcggtga
     6181 agtccggtgc cgtggcaccg attgcgagtg tcatcaggct tctcccctgt atgcgacgat
     6241 ggcggcccgt ggccgttgcc tcttgcctgg ccggccggct aggcgcaacc gtgccttggc
     6301 gccggccgcc tcaggcctaa gatggggcgc atgaccaagc gaataaaggg cgcaatgcca
     6361 atgccgaagc ggatcgtcac cctgaccctc aatccggcgg tcgacgcctc ctgcgccacc
     6421 gaggaggtgc gcccggtcaa caagctgcgc acgaccaacg accggctcga tcccggcggc
     6481 ggcggcatca acgtggcgcg ggtcgtcaac gagctcggcg gccagagctt cgcggtctat
     6541 gccgccggcg gccggtcggg cgacgtgctc gacgagctgg tgcgcgatgc cggcgtcatg
     6601 gcgcaccgca tcgccaccgc cgagccgacc cgggtgagcc atgtggtctt cgagcggcgc
     6661 tcgggccagg aataccgctt cacgccggag ggcccgatgc tcgccgaaag cgaatggcag
     6721 gccgccctcg ccttcgtcga gctgctcgac ttcgactacc tcgtcgccag cggcagcctg
     6781 ccgcgcggcg tgccggacga tctctacgcc cggctcgccg aggtggtgaa gaagcgtcgc
     6841 gccaggctcg tcgtcgacac ctccgggccg gcgctggccg ccgccctcgg cgccggcgtg
     6901 catctggtca agccgaaccg gagcgagttc gcccggctcc tggggcggga tctcggcgac
     6961 ccggccgagc tggcggcggc cgccaccgag ctggttgcgt cgggcagcac cgagctgctc
     7021 gccgtgacca tgggccacga gggtgcgctc ctcgccacgc cggacgggat ctggcggctc
     7081 ggcactcccg aggtcgaggc gaggagcgcg gtcggcgccg gcgacagctt cgtcgccggc
     7141 atgacctggg gcctcgccag cggggagagc gtcgagcgcg ccttcgccct cggcgtcgcc
     7201 gccggcaccg ccaccgtcct cacccccggc accgagctct gccacctggt cgacgtgcag
     7261 cgcttcttca gggatctccg acccgtcagg gcttgacgac gagcggccct gcaccatcta
     7321 ccatccacac ggatggttta tggatggtaa aaggatggtt gaaacgcccg ccgaacgctc
     7381 accccggacg cccgacagcg agaagatcac gctcaatctc ggctatgtcg atctcggccg
     7441 gatcgacctc ctggtgcagg agggcttcta cgccaaccgt agcgacttca tccgcacggc
     7501 catccgcaac cagctcgacc ggcatgccga ggcggtcgac cggtcggtcg gccgccaggg
     7561 gctcgacctc ggcctcaggc gctacagccg cgccgacctc gaggccatgc gggccgccgg
     7621 cgagatgctc cacatcaacg tgctcggcct tgccaccatc gccagcgacg tcacgcccga
     7681 gctggcccgc gccaccatcg cctccgtcac cgtcctcggt gccctcaacg ccacgccggc
     7741 ggtcaaggct gcactgcagg accgcatgca ctgatccccc agctcccgcg aaagccccaa
     7801 tcatgaacat gatcgactcc ggccgcatgg ccgaagccac ccggctgacc cgtgccggcc
     7861 ggctggccga ggcaacggcc ctgctgcagg gtgcgctggg cggcgccgcc acggcaccgc
     7921 cggcggacgg ctggcagggg ccgaccctcg acctccgggc cgagccgctg cccgaagcct
     7981 cgcccggtgg cgagcaatgg ctggcccgca gtcacgccaa tcctttcggg cggcgcgatt
     8041 accggctctt cgttccggcc gcggccgcca acgagcagcg gccgcttttg gtcatgctgc
     8101 acggctgcac gcagaatccg gacgatttcg ccgccggcac ggccatgaac cggctcgccg
     8161 ccggggccgg cctcatcgtc gcctggcccg gccagtgcca gacggccaac ccgcagcgct
     8221 gctggaactg gttccagccg accgaccagg agcgcgcccg gggcgagccc tcgatcatcg
     8281 ccggcatcac ccgcgaggtg atgcgcgacc acagcgtcga tgccggccgg gtctatgtcg
     8341 ccgggctctc ggccggcggt gcggaagccg ctgtcctggc cgcgacctat cccgatgtct
     8401 acgccgcctt cggcgtccat tccggcctcg cccacggggc tgcgcgcgat gtcgcctccg
     8461 cctttcaggc gatgcgcgag cccgccgccg gtcggcccgc gtcggtcggc ggccggaaga
     8521 tgccggtcat cgtcgtgcat ggcgaccgcg acgccacggt gcatccgggc aatggtgccg
     8581 ctgtgatccg ccaggccgtc ggaacgctgg agaccacggt cgagcgcgga cgcgtgccca
     8641 acggccacgc ctggacccgc acccggcacc tggatcaggc cggccagacc ctcgccgagc
     8701 actggcaggt gcacggcctc ggccatgcct ggtccggcgg caatgcggct ggcagctaca
     8761 ccgatccgcg cggccccgat gccagccgcg ccatgctcga cttcttcctc ggccatcgga
     8821 acgggcccgc cgccgccggt tgacgacccg tgaccggcgt gcgagcgctc gccggtcacc
     8881 cttgcccgag gaaattcccg ccatgcccgc cagcccgatt cgcaaccgcg ccgccgacct
     8941 gctcgcccgg cgcctcgccg aggccggctg ccgcttcgcc ttcggcattc cgggcg
//
LOCUS       DAOTFI010000202         8981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9280_length_8981_cov_7.129621, whole genome shotgun sequence.
ACCESSION   DAOTFI010000202 DAOTFI010000000
VERSION     DAOTFI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9280_length_8981_cov_7.129621"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="P5558_18030"
     CDS             <1..216
                     /locus_tag="P5558_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRY26275.1"
                     /translation="ILPDLFREGQGIVAQGTLVSAGAEPVLSASEILAKHDENYMPRE
                     VTDALKEAGYWQHNEAAGDAPADGVSQ"
     gene            213..2213
                     /locus_tag="P5558_18035"
     CDS             213..2213
                     /locus_tag="P5558_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRY26276.1"
                     /translation="MIAELGHFALVMALGLAIAQGSLPFLGAARHDLALMRLGSTAAV
                     GQLACVTLALAALTNAYVTSDFSLLNVYENSHSAKPLLYKISGVWGNHEGSLVLWVWI
                     LALFGAAVALFGRNLPLPFKARTLAVQGLIGTGFLLFMLFTSNPFERLVPAPLDGTGL
                     NPILQDPGLAFHPPMLYLGYVGLSVTFSFAMAALIEGRVDASWARWVRPWTLVAWVAL
                     TLGIALGSWWAYYELGWGGWWFWDPVENASFMPWLAATALLHSAIVVEKRDTLKSWTI
                     LLAILAFSLSLLGTFLVRSGVLTSVHAFAVDPSRGVFILALLVIAIGGSLAMFALRAP
                     AMQPGGHFAPVSREGGLLLNNLLLATATATVLLGTLYPLALEVVGGAKISVGPPFFNA
                     TFVPLMLPLLLAVPIGATLAWKRGNLMGAFRRLRLAVAAALATMALYLVLVSPQSLGA
                     ALGFGLAVWLIGGSVTDLAARVGLLELAPGRTWQRLKGLPASAFAVTIAHAGLGVLIV
                     GITASSTLQEERLLAMRPGETTTIGRYEVRFAGVDERQGPNYSALRATFETTSGGLPV
                     ATLHSEKRFFPVEGQPTTEAGIHTNLWRDLYFVLGDRTPDGAWAVRLYINPFVIWIWG
                     GVGIMALGGLVSLLDRRLRVGAPTTAAARRGGRPDLPDPVGA"
     gene            2231..2761
                     /locus_tag="P5558_18040"
     CDS             2231..2761
                     /locus_tag="P5558_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HRY26277.1"
                     /translation="MRRLLFVVPALVFVAIAGFLAIGLTRDPSTLPSALLDRPAPDFA
                     LPPLPGRDDAGFARADLGGGPMLVNFFASWCVPCRIEHPIINRLAEAGVTVHGINYKD
                     KPEDALAWLAELGDPFARVGRDQDGRVGIDWGVYGVPETYVIDADGTIVHKHVGPLQP
                     RDLERTILPLLESLKR"
     gene            2758..3231
                     /locus_tag="P5558_18045"
     CDS             2758..3231
                     /locus_tag="P5558_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRY26278.1"
                     /translation="MSRRLVLAFLLALAMALPVHAALTSDELLQDPVLEARARAIGKE
                     LRCLVCQNQSIDDSDADLARDLRHLVRERLLAGDSDAEIREFMVERYGEFVLLKPPVT
                     MGTLLLWGGPFVVLAIGGALIVLSLRRRRAASTTALTPDEESRLAELLDEGTGRP"
     gene            3228..4601
                     /gene="ccmI"
                     /locus_tag="P5558_18050"
     CDS             3228..4601
                     /gene="ccmI"
                     /locus_tag="P5558_18050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HRY26279.1"
                     /translation="MSGWSLLAGLGPLAIAVLALVLPLLRRRTADAAADSAVAILKDQ
                     LAELGRDVERGLLQPEEARAARVEIERRLLRAADRATPHAFEAGRIGRALVLVALVAV
                     PLGATALYLRLGSPSLPDRPFASRADERQGPAVPEQILTMVASLEARLATDGDDPEGW
                     LMLGRSRFVLGRLEPAIEAYRRALELAPEQAEAVAGLADALMARSQGVVTPEVQALMR
                     RLAALAPGDPRPPYFQGLAAAQAGDFNAALEQWRAMLEAAPADAPWRAQVEPSIRQAA
                     AELGLDPEPILALARTPTAEEDAAAAMAALPPEERQARIRAMVDGLQSRLETSGGSVD
                     EWQRLGQARLVLGERDAAIAAYREALAQAPDNPAALKGLAAALVDGPAEPDGLPVVGD
                     EALALFEQAATVAPDDPEPHWYLGIRALADGNPATAREHWETVLAGLDPTSPDWATVR
                     ARLDQLD"
     gene            complement(4609..5058)
                     /gene="moaE"
                     /locus_tag="P5558_18055"
     CDS             complement(4609..5058)
                     /gene="moaE"
                     /locus_tag="P5558_18055"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HRY26280.1"
                     /translation="MIRVQAGLFDVGAELARFTAGRTDIGAVASFTGLVRDRHNGEAV
                     TAMTLEHYPGMTEAELERIEAEARARWPLDDCLVIHRYGRMLPGEPIVLVLTASAHRA
                     AAFEACAFLMDWLKTKAPFWKLEETPEGERWVEARASDDEAATRWER"
     gene            complement(5060..5311)
                     /gene="moaD"
                     /locus_tag="P5558_18060"
     CDS             complement(5060..5311)
                     /gene="moaD"
                     /locus_tag="P5558_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HRY26281.1"
                     /translation="MRVRYFAWLKQRTGCAEEELDLPAEVLTVADLIGHLGARHPRFA
                     EAAAGRGVVRCAIDQQHVEREAPLAGAREVAFFPPVTGG"
     gene            complement(5316..7172)
                     /gene="uvrC"
                     /locus_tag="P5558_18065"
     CDS             complement(5316..7172)
                     /gene="uvrC"
                     /locus_tag="P5558_18065"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRY26282.1"
                     /translation="MDQPSASGAALIHGLLANLPNVPGVYRMIDARGNVLYVGKAKSL
                     KKRVATYAKAHALPIRLQRMVALTTAMEFVTTASEVEALLLEANLIKRYRPTFNIVLR
                     DDKSFPYLYLRWGHDFPMIGKHRGSKRDGSDYFGPFASAGAVNDTLNALLRAFPLRSC
                     SDSIFASRSRPCLQHQIKRCSAPCVGRIEKADYARIVEDVRGFLGGSTRDVQGKLKAE
                     MEEAAGRLDFEKAAVLRDRLKAIAHISSRQGINTSAVEDADVIAAHADGGQVCVQAFF
                     YRGGRNYGNRAYYPAHARDADMPEVMAAFLAQFYSERPPPRLVLVGTEPAEAGLLEEA
                     LRLKAGRRVHVRMPKRGPLRELVELARRNAEAALARRLADQASQARLLEQLAERFDLD
                     AVPERVEVYDNSHIQGSHAVGSFVVAGPEGFDRKAYRTFTIKDPATGTNDDYAMMREV
                     LERRFARLAREDPERQSGQWPDLVVIDGGAGQLSAAQGVLDQLGIEGVALLGVAKGPD
                     RDAGRERFFRTGLDPVMLDARDPVLYLLQRWRDEAHRFVIQAHRGKRSRAIGQSTLDA
                     IPGIGGKRKKALLQHFGSARDVESAALGDLEQVPGISSAVARVIYDHFHEGG"
     gene            complement(7270..8166)
                     /locus_tag="P5558_18070"
     CDS             complement(7270..8166)
                     /locus_tag="P5558_18070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HRY26283.1"
                     /translation="MAFAERGTTEQANRKIIRDSMNTPLLSREHELELARAWRNEGDE
                     VALHELIKAYMRLVVSAASRFKNYGLPMSDLIQEGVVGLMQAAARFEPERDVRFSTYA
                     TWWIRSAMQDYILRNWSVVRTGTTAAQKSLFFNLRRLRAKIDDGLGSSLTEEGRASIA
                     ETLSVSVNDVAAMEMRLMGGDQSLNATISLTGEDVWQDFLADERPNPEDVVSDRHDSK
                     ARTAWLRDSLKELNERERTIIRERRLREDGRTLEELGRRLGISKERVRQIEHRALQKL
                     KVALMRKVKEPGDELLLTAVPA"
     gene            complement(8353..>8981)
                     /gene="mtnA"
                     /locus_tag="P5558_18075"
     CDS             complement(8353..>8981)
                     /gene="mtnA"
                     /locus_tag="P5558_18075"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRY26284.1"
                     /translation="TVNVLTHCNAGWLATIDQGTATAPIYAAQAKGIPLHVWVDETRP
                     RNQGAALTAFELLQQQVPHTVVVDNAGGHLMQHGKVDLCIVGTDRTTATGDVCNKIGT
                     YLKALAAKDNGVPFYVALPSTTIDWTIRDGVSEVPIEERAAEEVTVMSGRTADGRIES
                     VRITPEGSPAANFAFDVTPARLVSGLITERGVAEASPEGLRRLFPDAP"
BASE COUNT         1169 a         3341 c         3041 g         1430 t
ORIGIN      
        1 atcctgccgg acctcttccg cgaggggcag ggcatcgtcg cccagggcac gctcgtgtcc
       61 gccggcgccg aaccggtcct gagcgccagc gagatcctgg cgaagcacga cgagaactac
      121 atgccgcgcg aggtcacgga cgccctgaag gaggccggtt actggcagca caatgaagcg
      181 gcgggcgacg cgccggccga cggggtgagc cagtgatcgc cgagctcggt catttcgcgc
      241 tggtcatggc gctcggcctc gccatcgccc agggcagcct gccgttcctc ggtgccgccc
      301 gccacgacct ggcgctgatg cggctgggct ccacggcggc ggtcggccag ctcgcctgcg
      361 tcaccctggc gctcgctgcc ctcaccaacg cctacgtcac gtccgatttc tcgctgctca
      421 atgtctacga gaacagtcac tcggccaagc cgctgctcta caagatcagc ggcgtctggg
      481 ggaatcacga gggctcgctc gtcctctggg tctggatcct cgccctcttc ggcgccgccg
      541 ttgccctttt cggccgcaac ctgcccttgc cgttcaaggc ccgcacgctc gccgtgcagg
      601 ggctgatcgg cacgggattc ctgctcttca tgctcttcac ctccaacccc ttcgagcggc
      661 tggtgccggc gccgctggac ggcaccggcc tcaacccgat cctccaggat cccggcctcg
      721 ccttccatcc gcccatgctc tacctgggat atgtcggcct ctcggtgacc ttctccttcg
      781 ccatggcggc cctgatcgag ggccgggtgg acgcgagctg ggcacgctgg gtgcggccct
      841 ggacgctggt cgcctgggtc gcgctcacgc tcggcatcgc gctcggctcc tggtgggcct
      901 attacgagct cggctggggc ggctggtggt tctgggaccc ggtcgagaac gccagcttca
      961 tgccctggct cgcggccacg gccctgctgc attcggcgat cgtcgtcgag aagcgcgaca
     1021 cgctgaagag ctggaccatc ctgctcgcca tcctcgcctt ctcgctctcg ctgctcggca
     1081 ccttcctcgt ccgctcgggc gtgctcacct cggtccatgc cttcgccgtc gatcccagcc
     1141 gcggcgtctt catcctggcg ctcttggtga tcgccatcgg cggctcgctc gccatgttcg
     1201 cgctccgggc acccgccatg cagccgggcg gccatttcgc cccggtcagc cgcgagggcg
     1261 ggctgctttt gaacaacctc ctcctggcga cggcaaccgc cacggtgctg ctcggcaccc
     1321 tctacccgct ggcgctcgag gtcgtcggcg gggcgaagat ctccgtcggg ccgcccttct
     1381 tcaacgccac cttcgtgccg ctcatgctgc cgctcctgct cgccgtgccg atcggcgcca
     1441 cgctcgcctg gaagcgcggc aacctcatgg gggcctttcg tcgcctccgc ctcgcggtgg
     1501 cggcggccct ggccaccatg gcgctctatc tcgtgctggt gtcgccgcag agcctcggtg
     1561 cggcgctggg cttcggcctc gcagtctggc tgatcggcgg gtccgtcacc gacctcgccg
     1621 cccgggtcgg gctcctcgag ctcgcacccg gccggacctg gcagcgtctg aaggggctcc
     1681 ccgccagcgc cttcgccgtc accatcgccc atgccgggct cggcgtgctg atcgtcggca
     1741 tcaccgcgtc cagcacgctg caggaggagc gtctcctcgc gatgcggccg ggcgagacca
     1801 ccacgatcgg ccgctacgag gtgcgattcg ccggcgtcga tgaacgccag gggccgaact
     1861 actcggcgct ccgcgccacc ttcgagacca ccagcggcgg cctgccggtc gccacgctcc
     1921 actcggaaaa acgcttcttc ccggtcgagg gccagccgac caccgaggcc ggcatccaca
     1981 ccaatctctg gcgcgatctc tacttcgtgc tcggcgaccg gacccccgac ggggcctggg
     2041 cggtccgcct ctacatcaac cccttcgtca tctggatctg gggcggggtc ggcatcatgg
     2101 cgctgggggg gctcgtctcc ctcctcgacc ggcgcctcag ggtcggcgcg ccgacgacgg
     2161 cggcggcccg gcggggcggc cggccggatc tccccgaccc cgtcggcgcc tgaacggagc
     2221 tgcatcgcgc atgcgtcgcc tcctgttcgt cgtcccggcc ctggtcttcg tcgccatcgc
     2281 cggcttcctc gccatcggcc tgacccgcga tccgtccacc ttgccctcgg ccctgctcga
     2341 ccgcccggcc cccgacttcg cgctgccccc cttgccgggt cgcgacgacg ccggcttcgc
     2401 ccgggccgat ctcggcggcg ggccgatgct cgtcaacttc ttcgcctcct ggtgcgtgcc
     2461 ctgccggatc gagcacccga tcatcaaccg gctggccgag gcgggcgtga cggtgcatgg
     2521 catcaactac aaggacaagc ccgaggacgc gctcgcctgg ctcgccgagc tgggcgatcc
     2581 cttcgcccgc gtcggccgcg accaggacgg ccgggtcggc atcgactggg gcgtctacgg
     2641 cgtgcccgag acctatgtga tcgatgccga cggcaccatc gtccacaagc atgtgggccc
     2701 gctgcagccg cgcgacctcg agcggaccat cctgccactc ctcgagagcc tgaagcgatg
     2761 agccgccgcc tcgtcctcgc gttcctcctg gcgctggcca tggccctgcc ggtgcatgcc
     2821 gccctcacct cggacgagct gctccaggac ccggtgctcg aggcacgggc gcgcgccatc
     2881 ggcaaggagc tgcgctgcct cgtctgccag aaccagtcga tcgacgattc cgatgccgat
     2941 ctcgcccgcg acctccgcca cctcgtgcgc gagcggctcc tggccggcga cagcgatgcc
     3001 gagatccgcg agttcatggt cgagcgctat ggcgagttcg tcctcctcaa gccgccggtg
     3061 accatgggca ccctcctgct ctggggcggg ccgttcgtgg tgctggcgat tggcggtgct
     3121 ctcatcgttc tgagcctgcg ccgtcgccgt gccgccagca cgacggcgct gacgccggac
     3181 gaggaaagcc ggctggccga gctgctcgac gagggtaccg gtcgtccatg agtggctgga
     3241 gcctgctcgc cggcctcggg ccgctggcga tcgccgtcct ggcgctcgtc ctcccgctcc
     3301 tgcggcgacg cacggccgac gcggctgccg acagtgcggt ggccatcctc aaggaccagc
     3361 tcgccgagct cggccgcgac gtggagcgag gcctgctcca gcccgaggag gcgcgcgccg
     3421 cccgggtcga gatcgagcgc cggctgctgc gggcggcaga ccgcgcgacg ccgcacgcgt
     3481 tcgaggccgg ccggatcggc cgcgccctgg tgctcgtggc cctggttgcc gtcccgctcg
     3541 gcgcgaccgc cctctatctc cggctcggct cgccgagcct gccggatcga cccttcgcgt
     3601 cgcgcgccga cgagcggcag ggcccggccg tgccggagca gatcctgacc atggtcgcca
     3661 gtctcgaggc gcgtctcgcc acggatggtg acgatcccga gggctggctg atgctcggcc
     3721 gcagccgctt cgtgctcggc cggctggagc cggcgatcga ggcctaccgg cgggcgctcg
     3781 agctggcgcc ggagcaggct gaagcggtgg ccggcctcgc cgacgcgctc atggcccgct
     3841 cgcagggcgt ggtcacgccc gaggtgcagg cgctcatgcg gcgcctcgcc gcactcgccc
     3901 cgggtgaccc gcgaccgcct tacttccagg gtctcgccgc ggcccaggcc ggcgacttca
     3961 acgccgctct ggagcaatgg cgcgccatgc tcgaggcggc acccgccgat gctccctggc
     4021 gtgcgcaggt cgagccctcg atccggcagg cggccgccga gctcggcctc gatcccgagc
     4081 cgatcctggc tctcgcccgc acgcccacgg ccgaggagga tgccgccgcc gccatggcgg
     4141 ccctgccgcc cgaggaacgc caggcgcgga tccgggcgat ggtcgacggg ctccagagcc
     4201 ggctcgaaac ctcgggcggc agcgtcgacg agtggcagcg cctgggccag gcccgcctcg
     4261 tgctcggcga gcgcgacgcg gcgatcgccg cctatcgcga ggcgctggcg caggcacccg
     4321 acaacccggc ggcgctgaag ggtctcgccg ccgccctggt cgacggcccg gccgagccgg
     4381 atggcctgcc cgtggtcggc gacgaggcgc tggccctctt cgagcaggcg gccaccgtgg
     4441 cgcccgacga tccggagccg cactggtatc tcggcatcag ggcgctggcc gatggcaatc
     4501 ccgcgacggc tcgcgagcac tgggagaccg tgctcgccgg gctcgatccg acgagccccg
     4561 actgggccac ggttcgcgcc cgcctcgacc agctcgactg acggcgcttc agcgttccca
     4621 gcgggtcgcg gcttcgtcgt cgctggcccg cgcctcgacc cagcgctcgc cctccggcgt
     4681 ctcctcgagc ttccagaacg gggccttcgt cttgagccag tccatgagga aggcgcaggc
     4741 ctcgaaggcg gcggcgcgat gggcggaggc cgtcagcacc agcacgatgg gctcgcccgg
     4801 cagcatccgc ccgtagcggt ggatgacgag gcagtcgtcc agcggccagc gggcgcgggc
     4861 ctcggcctcg atccgttcca gctcggcctc ggtcatgccc gggtagtgct cgagcgtcat
     4921 cgccgtcacg gcctcgccgt tgtggcggtc gcgcacgagg ccggtgaagc tcgccaccgc
     4981 accgatgtcg gtgcggccgg cggtgaaccg ggcgagctcg gcgccgacgt cgaagagccc
     5041 ggcctgcacc cggatcatct cagccgccgg tgaccggcgg gaagaaggcg acctcgcggg
     5101 ccccggcgag tggcgcctcg cgctcgacat gctgctggtc gatggcgcag cggacgacgc
     5161 cgcggccggc cgccgcctcg gcgaagcgcg ggtggcgggc acccagatgg ccgatcaggt
     5221 cggccacggt cagcacctcc gccggcaggt cgagctcctc ctcggcgcag ccggtgcgct
     5281 gcttgagcca ggcgaaatag cgaacacgca tgaacttagc cgccttcgtg aaagtgatcg
     5341 tagatgacgc gcgccaccgc actgctgatg ccgggcacct gctcgagatc gccgagcgcc
     5401 gcgctctcca catcccgggc cgagccgaaa tgctgcagca gcgccttctt ccgcttgccg
     5461 ccgatcccgg gaatcgcatc cagggtcgac tggccgatgg cccggctgcg cttgccgcga
     5521 tgcgcctgga tgacgaagcg atgcgcctcg tcgcgccagc gctggaggag gtagagcacc
     5581 gggtcgcggg cgtccagcat caccgggtcg agcccggtgc ggaagaagcg ctcgcgcccg
     5641 gcatcgcgat ccggcccctt ggcgacgccg agcagggcca ccccctcgat gccgagctgg
     5701 tcgagcacgc cctgggccgc cgagagctgc cccgcaccgc cgtcgatgac gacgagatcc
     5761 ggccactgcc cggactgccg ctccgggtcc tcgcgcgcca gccgggcgaa gcgccgctcc
     5821 agcacctcgc gcatcatggc gtagtcgtca ttcgtgccgg tcgccggatc cttgatcgtg
     5881 aaggtccggt acgccttccg gtcgaagccc tcgggcccgg cgacgacgaa gctgccgacg
     5941 gcgtggctgc cctggatgtg gctgttgtcg tagacctcga cccgttccgg caccgcgtcg
     6001 aggtcgaagc gctccgctag ctgctcgagc agccgggcct gcgaagcctg gtcggcgagc
     6061 cttcgggcga gggccgcctc ggcgttccgg cgggcgagct cgaccagctc gcgcaagggg
     6121 ccgcgcttcg gcatgcggac atgcacgcga cgccccgcct tgagtctcag cgcctcctcg
     6181 agcagcccgg cctcggccgg ctcggtgccg accaggacga gccgcggcgg cgggcgctcg
     6241 ctgtagaact gggcgaggaa cgcggccatg acctcgggca tgtcggcgtc gcgcgcatgc
     6301 gccggatagt aggcgcggtt gccgtagttc cgcccgcccc ggtagaagaa cgcctggacg
     6361 cagacctgcc cgccatcggc gtgcgcggcg atgacatcgg catcctcgac agcactggtg
     6421 ttgatgccct ggcggctgct gatatgggcg atggccttca gccggtcgcg cagcaccgcc
     6481 gccttctcga agtcgagccg gccggccgcc tcctccatct ccgccttgag cttgccctgc
     6541 acgtcgcgcg tgctgccgcc gagaaagccc cgcacgtcct cgacgatgcg cgcgtagtcc
     6601 gccttctcga tgcggccgac gcagggtgcg gagcagcgct tgatctggtg ctgcaggcag
     6661 ggccggctgc ggctggcgaa aatcgagtcg ctgcagctcc gcaagggaaa ggcgcgaagc
     6721 agcgcgttca gcgtgtcgtt gacggcgccc gccgaggcga acgggccgaa atagtccgag
     6781 ccgtcgcgct tgctgccccg atgcttgccg atcatcggaa agtcatgacc ccagcggaga
     6841 tagagatagg gaaagctctt gtcgtcgcgc agcacgatgt tgaaggtcgg gcgatagcgc
     6901 ttgatcaggt tcgcctcgag cagcagcgcc tcgacctcgc tcgccgtcgt cacgaactcc
     6961 atggctgtcg tcagcgccac catgcgctgc agccggatcg gcagcgcatg cgccttggca
     7021 taggtggcca cccgcttctt cagcgacttg gccttgccca catagaggac gttgccgcgc
     7081 gcatcgatca tgcggtagac gcccggcaca ttgggcaaat tggccaggag gccgtggatc
     7141 agcgcagcac cgctggcgct gggctgatcc atggttgtat cggcagtgac gaccggcggc
     7201 atgacccgct atctgcgcat cgcccggccc cgggtcaagc cgccggcgcc tgcgccgggc
     7261 agccgagtgt caggccggca cggcggtcag cagcagctcg tcgcccggct ccttgacctt
     7321 gcgcatcagg gcgaccttca gcttctgcag ggcacggtgc tcgatctgcc gcacgcgctc
     7381 cttgctgatg ccgagccgcc ggccgagctc ctcgagcgtc cgcccgtcct cgcgcagccg
     7441 ccgctcgcgg atgatcgtgc gctcgcgctc gttcagctcc ttgagcgagt cgcgcagcca
     7501 cgccgtccgt gccttgctgt cgtgccggtc gctgaccacg tcctccgggt tggggcgctc
     7561 gtcggcgagg aagtcctgcc agacatcctc gccggtcagg ctgatggtgg cgttgagcga
     7621 ctggtcgcca cccatcagcc gcatctccat ggcggccacg tcgttgaccg agaccgacaa
     7681 tgtctcggcg atcgaggcgc gaccctcctc cgtgagcgag ctgccgagac cgtcgtcgat
     7741 cttggcgcgc aggcgacgca ggttgaagaa cagcgacttc tgcgcggcgg tggtgccggt
     7801 ccggaccacc gaccagttgc gcaggatgta gtcctgcatc gccgagcgga tccaccaggt
     7861 ggcataggtc gagaagcgca cgtcgcgctc cggctcgaac cgggccgccg cctgcatcag
     7921 gccgaccacg ccctcctgga tgaggtcgct catcggcagg ccgtaattct tgaaccggct
     7981 ggcagcgctc accacgaggc gcatataggc cttgatcagc tcgtgcagcg ccacctcgtc
     8041 gccctcgttg cgccaggccc gggccagctc gagctcgtgc tcgcgcgaca gcaggggcgt
     8101 gttcatggag tcccggatga tcttccggtt cgcctgctcg gtcgtgccgc gttcagcaaa
     8161 cgccatgcgc ttcctccttc accaggtcgg ttcgatccgc gtaccgcggc acaccgacac
     8221 tcggccccta acggccgagg atgcccttgg tcccctattc acgtcggtct acgccgaatt
     8281 attgccgcag gatcatcgca tgaccgaagt ttcacggtgt cgtgatgtgg cgttcagcca
     8341 accgcccgac gctcacggag cgtccgggaa gagccggcgc aggccctccg ggctcgcctc
     8401 ggccacgccg cgctccgtga tcaggcccga gaccagccgg gcgggcgtca cgtcgaaggc
     8461 gaaattcgcc gccgggctgc cttcgggcgt gatgcgcacg ctctcgatcc gcccgtcggc
     8521 cgtccgcccg ctcatcaccg tcacctcctc ggcggcacgc tcctcgatcg gcacctcgct
     8581 caccccgtcc cggatggtcc agtcgatcgt cgtcgacggc agcgccacgt agaacggcac
     8641 gccgttgtcc ttggccgcca gcgccttcag gtaggtgccg atcttgttgc agacgtcgcc
     8701 cgtggcggtc gtccggtcgg tgccgacgat gcagaggtcg accttgccgt gctgcatcag
     8761 gtgaccgccg gcattgtcga cgaccacggt gtgcggcacc tgctgctgca gcagctcgaa
     8821 ggcggtgagc gctgccccct ggttgcgcgg ccgggtctcg tcgacccaga catgcaacgg
     8881 aatccccttc gcctgcgccg cgtagatggg ggcggtggcc gtgccctggt cgatggtggc
     8941 gagccagccg gcattgcagt gcgtcaggac attgacggtc t
//
LOCUS       DAOTFI010000203         8974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9286_length_8974_cov_6.767463, whole genome shotgun sequence.
ACCESSION   DAOTFI010000203 DAOTFI010000000
VERSION     DAOTFI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9286_length_8974_cov_6.767463"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="P5558_18080"
     CDS             complement(<1..825)
                     /locus_tag="P5558_18080"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRY26285.1"
                     /translation="MATDTTQWIGRKAEAQDVLHAERVAALWAALDRGGPPPGAGEAL
                     PPLWHWLYFWALPRRSDLGPDGHPARGGIVPDLPEGRRMWAGSSVTFHRPLAIGAAVT
                     RCSTVATVTEKTGRGGRLVFIEVAHEIGDEDGPALTDRHHIVYRPPPDGTPPRAPEPA
                     PATADRAETWQADSSLLFRYSALTYNGHRIHYDHPYATREEGYPGLVVHGPLLATLMA
                     ANAQALHPDRPLASFAFRATAPVFANEPFTVMADRRGAVWVRRADGGYATRGEVAWA"
     gene            complement(834..1991)
                     /locus_tag="P5558_18085"
     CDS             complement(834..1991)
                     /locus_tag="P5558_18085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY26286.1"
                     /translation="MDLDADYPEIREAVARICAEFPSTYWRELDAQGAYPTGFVQALS
                     KSGYLGALIPEEHGGAGLPLRAAAVILETIHERGASAAACHAQMYTMGTLLRHGSAEQ
                     KARYLPEIAAGGLRLQAFGVTEPTSGSDTPMLKTRAERRGDKWIVNGQKIWTSRALHS
                     DLMLLLVRTTPIEECTKRSQGLSVLLVDLREARGQGLDIRPIDTMINHNTTEVFIADL
                     EVPAANLIGVEGEGFRYILDGMNAERILLSGEAIGDGRWFIERATAYANERRVFGRPI
                     GQNQGVQFPIARAHAQLEAAELMARKAAALFDAGLPCGPEANMAKLLTSEAAWAAGEA
                     CFATFGGFAFAREYDIERKWRECRLFQTAPISTNMILAFIAQNVLGLPRSY"
     gene            complement(1981..2901)
                     /locus_tag="P5558_18090"
     CDS             complement(1981..2901)
                     /locus_tag="P5558_18090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRY26287.1"
                     /translation="MTFELRHISVFVAVAEELSFRRAALRLGVAQPAVSRLIAELETR
                     LAVPLLRRTTRSVALTEPGRYFLDEARQILARVERTTAVSRGLASGAFGRLAVAYMSI
                     AAHTVVPHVVQAYRRHHPDVALNLTYMGSEQQRDAVMRGAIDVGFVVGPFHSPEIDGR
                     LVSRHRLVAIMPAGHRLADRAALTPADLVGEPLILGPRDDWGAFRRLVTEVFEAAGVV
                     PHVALEATSITALFGLVASGLGIILFAGFPRQYDRAQFLPKPILTDRHPHLLTHVVWR
                     RDTLNPAVGPFVETALRAAPELPTSDFDGP"
     gene            3009..3713
                     /locus_tag="P5558_18095"
     CDS             3009..3713
                     /locus_tag="P5558_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRY26288.1"
                     /translation="MADQSSAVVSDAELREALEVVFRANSALYKERGFQRRLGFGKRP
                     ALIVIDLAKAWTLPGHAFSCADMDTIIPANQKLLEAFRARGFPVVFTTTAYDITDGPN
                     SDMGLWGQKIPLETLSADSEWVAIDDRIAPQPGELVIVKKKASAFHGTHLASYLTANR
                     VDTVIVTGVTACACVRHTIEDAIADGFRPIAVREGIGDRIAGAVEWNLFDIDAKFGDV
                     EPLERVLQYLQSNEIG"
     gene            3751..5844
                     /locus_tag="P5558_18100"
     CDS             3751..5844
                     /locus_tag="P5558_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRY26289.1"
                     /translation="MIRVGVDVGGTFTDIVLERTRAAGEDQQIVVHKVPSTPQDQSEG
                     VVKGVVEICQMAGIAPGEIDMVFHGTTVATNMVIERKGAEVGMITTRGFRDILHMARH
                     KRPHNFSLQFDVPWQSKPLVKRRNRLPVTERIMPPDGRVEVPLDTDEVKAAAELFAKR
                     GLDAVIVGFLFAFLNDEHEQKAKAIVEAAMPDAYVCCSSEIVNVIREYERFSTCAMNA
                     YIGPMTARYMRQLEGRLRAAGVRAAVRIMQSNGGISTVENAIQRPVGLLLSGPAGGVI
                     GGRWTGEHAGTQNVITIDIGGTSADISVIQGGELRIKNPRDTEVASLPVLVPMIDVDA
                     IGAGGGSIAYLDPGGAFRVGPRSAGADPGPACYDKGGDEPTVTDAQVVLGRLDPEQFL
                     GGDLAIEPSLSERAIAKHIAGPMGLSVTEAALGILRIINNNMALAIHNASVAKGIDPR
                     NFTLMGFGGAGPLHATALAELISARDVIAPLQPGITAAMGLLVTDLQYEYTHSVLLVL
                     NQAGEAEFAALNAAMDELRAQAEAQLEADGIAPGARRFRKVAECRYLGQGFELRADMP
                     EGPLAKDNVQEVIRSFFDAHQQVYGHAFEDQLTEAVTLRLIATAEVDMLRLPDLAQGG
                     RTDPKDAVLYVRDTVFDDGEARRTPRYRRDRLLAGDRVTGPALIVQHNSTTLLPPGYA
                     AEVLGHGDIRVAKRA"
     gene            5855..7630
                     /locus_tag="P5558_18105"
     CDS             5855..7630
                     /locus_tag="P5558_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY26290.1"
                     /translation="MTREVDPITLQVIGGALHAIAEQMGNVLYRMSYSSIIRESQDLG
                     AGLFDRDFNTLCESDSTPMHIGSLPGYLRGIARTIPIDGWQPGDCVIHNHPYAGASHS
                     PDIAIVMPVFFEGELIGFSANTAHHVDIGAATPGLIIDVPDMYAEGMLMDGLKLFEAG
                     KRNEALWKFISDNTRVPSLVKGDLEAQIASAELGVRRFQELLATYGRDQVIQATRQLM
                     DYTESMLRREIAKIPDGDYTAEGFLDDDGRNRGKHLPIKVTVKVRGDGVEVDLTGSSD
                     QVPTAFNVPFEGSTNVACFFAFRALLLDTYTHEEHIPQNEGSFRPVKVTAPLGSIYNP
                     IAPVAAEARFCQIQRVVDLIIKALAPVIPEKATAGNAATLSFAAFSGVRPSGDYWVFL
                     EVNEAAMGGRPTSDGPDTVEELMRNTRNNPLEDLGMHLPLICDRYEVRDDVAPGAGRF
                     RGGAGVVKAQRYLTPGFMTHESDRHDDAPWGIFGGKQGAVGKMEIYNVARPDEIASEP
                     AKFSGRRTQPGDVVAYFSPSGGGYGDPLERDPAKVLDDVLDGFITVQHALDDYGVVLD
                     AVDDGYGWQVDAEATWARRNEMRAG"
     gene            7752..>8974
                     /locus_tag="P5558_18110"
     CDS             7752..>8974
                     /locus_tag="P5558_18110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY26291.1"
                     /translation="MTEKKLEDATPPAAAGSGLSRRSILKGTAAATGLAVGSGAITGF
                     PTIWAQNIKDITLNHTGMSYSTLIDIARQASADLGFTVEMSVTDHPGLTNRMINDPGS
                     IDIADAEIWQAKVFVPQGVTQAVDVKRINLWDEFTPIYTKGEFLGRPVSRAGDSPFEF
                     MYLDAIDGTTFVEGPTEFATFMPGVFNADTLGIRADLVGRPIEKWGDLFSEEFKGKAA
                     IQNIPTIGIMDAIMACESAGIVTYGDKGNPTREELEATIAKLIELKRAGHWRALWNTF
                     DESVNLMASGEVVIQSMWSPAVTAVRTQGIDCVYQPLKEGYRGWGNGLALLSHLEGIK
                     LDAAYEYLNWYVSGWTGGFIAKQGYYISVPETAKDHLTENEWGFFYLGKPATADIVSP
                     TGVKTNSAGDVRDGGS"
BASE COUNT         1444 a         3054 c         3160 g         1316 t
ORIGIN      
        1 ggcccaggcg acctcgcccc gcgtggcgta gccgccgtcg gcgcgccgca cccagacggc
       61 accgcggcga tcggccatga cggtgaaggg ctcgttcgcg aagaccgggg cggtggcgcg
      121 aaaggcgaag ctggcgagcg gccggtcggg gtggagcgcc tgggcgttgg cggccatcag
      181 cgtggcgagg aggggcccgt ggacgacgag gccgggatag ccctcctcgc gcgtggcata
      241 ggggtggtcg tagtggatgc ggtggccgtt ataggtgagg gccgagtacc ggaaaagcag
      301 gctggagtcg gcctgccagg tctcggcgcg gtcggcggtg gccggggccg gctccggcgc
      361 gcggggcggc gtgccgtccg gcggcgggcg gtagacgatg tggtggcggt cggtgagggc
      421 agggccgtct tcgtcgccga tctcgtgcgc cacctcgatg aagacgagcc ggccgccgcg
      481 cccggtcttc tcggtgacgg tcgcgaccgt cgagcaacgg gtcacggcgg caccgatggc
      541 gagcgggcgg tggaaggtga cgctgctgcc ggcccacatg cggcgaccct cgggcaggtc
      601 ggggacaatg ccgccccgcg ccggatggcc gtcggggccg aggtccgagc ggcggggcag
      661 cgcccagaaa tagagccagt gccagagcgg cggcagcgcc tcgcccgcgc cgggcggcgg
      721 cccgcccctg tcgagggcgg cccagagggc ggccacccgc tcggcgtgca gcacgtcctg
      781 cgcctccgcc ttccggccga tccactgcgt cgtgtcggtc gccatcttgg acctcaatag
      841 ctgcggggca ggcccagcac gttctgggcg atgaaggcga ggatcatgtt cgtcgagatg
      901 ggggcggtct ggaacaggcg gcattcccgc cacttgcgct cgatgtcgta ctcgcgggcg
      961 aaggcgaagc cgccgaaggt ggcgaagcag gcctcgccgg cggcccaggc ggcctcgctg
     1021 gtgagcagct tcgccatgtt ggcctcgggg ccgcagggca gcccggcatc gaagagggcg
     1081 gccgccttcc tcgccatgag ctcggccgcc tcgagctggg cgtgggcgcg ggcgatgggg
     1141 aactgcacgc cctggttctg gccgatgggg cggccgaaga cccggcgctc gttcgcatag
     1201 gcggtggccc gttcgatgaa ccagcgcccg tcgccgatgg cctcgccgga gagcaggatg
     1261 cgctcggcgt tcatgccgtc gaggatgtag cgaaagccct caccctcgac gccgatgagg
     1321 ttcgcagccg gcacttcgag gtcggcgatg aagacctcgg tggtgttgtg gttgatcatc
     1381 gtgtcgatcg gccggatgtc gaggccctgg cccctggcct cgcggagatc gaccagcagg
     1441 accgagaggc cctggctgcg cttcgtgcat tcctcgatcg gggtggtgcg gacgagcagc
     1501 agcatgaggt cggaatggag ggcgcggctg gtccagatct tctggccgtt gacgatccac
     1561 ttgtcgcccc gccgctcggc ccgggtcttc agcatgggcg tgtccgagcc gctcgtcggc
     1621 tcggtcacgc cgaacgcctg gaggcggagc ccgccggccg cgatctcggg caggtagcgc
     1681 gccttctgct cggccgagcc gtgcctgagc agcgtgccca tggtgtacat ctgggcatgg
     1741 caggcggcag cgctggcgcc gcgctcgtgg atggtctcga ggatcaccgc ggcggcgcgc
     1801 aagggaagcc cggccccgcc atgctcctcg gggatgaggg ccccgagata gcccgacttc
     1861 gagagcgcct ggacgaagcc cgtgggatag gcgccctggg cgtcgagttc ccgccaatag
     1921 gtgctcggaa attcggcgca gatccgcgcg acggcctcgc ggatctccgg atagtcggcg
     1981 tcaaggtcca tcgaagtcac tggtcggaag ctccggtgcg gcgcgcagcg ctgtctcgac
     2041 gaaggggccg acggccgggt tcagcgtgtc gcgccgccag acgacgtggg tcaaaaggtg
     2101 gggatggcgg tcggtgagga tcggcttcgg caggaactgc gcccggtcgt actgccgcgg
     2161 gaagccggcg aagaggatga tgccgaggcc gctcgccacg aggccgaaga gggcggtgat
     2221 gctggtcgct tccagcgcca cgtgcggcac gacgccggcc gcctcgaaga cctcggtgac
     2281 gaggcggcga aaggcgcccc agtcgtcgcg tgggccgagg atcaggggtt cgcccacgag
     2341 atcggccggg gtgagggcgg cccggtcggc gagccggtgg ccggccggca tgatggcgac
     2401 gaggcggtgc cggctgacca gccgcccgtc gatttcgggc gaatggaagg ggccgaccac
     2461 gaagccgaca tcgatggcac cgcgcatcac cgcgtcgcgc tgctgctcgg agcccatgta
     2521 ggtcaggttg agggcgacgt cgggatggtg ccgccgatag gcctggacga cgtgcggcac
     2581 gacggtgtgc gcggcgatcg acatgtaggc gacggcgagc cggccgaagg cgcccgaagc
     2641 gaggccgcgg ctgaccgccg tcgtgcgctc gacgcgcgcc aggatctgcc tcgcctcgtc
     2701 gaggaaatag cggccgggct cggtcagcgc cacggagcgc gtggtccgcc gcagcagcgg
     2761 cacggcgagc ctcgtctcga gctcggcgat cagccggctg acggcgggct gggcgacacc
     2821 gagcctgagc gctgcccgcc ggaagctcag ctcctcggcg accgcgacga acaccgagat
     2881 gtggcgaagc tcgaaggtca attgatcttc cttcggcatc aattttgcac aaatcagtat
     2941 ttcccagacg gcgttcggtt gtctagcctt gctcgcaggc gttcttgtca tgcgggagta
     3001 tcataccaat ggcggatcaa tccagcgccg tcgtgtccga tgccgagctc cgtgaggcgc
     3061 tcgaggtggt gttccgggcc aattcggcgc tctacaagga gcgcggcttc cagcgtcgcc
     3121 tcggcttcgg caagcggccg gcgctcatcg tcatcgacct cgccaaggcc tggaccctgc
     3181 cgggccacgc cttctcctgc gccgacatgg acacgatcat cccggcgaac cagaagctgc
     3241 tcgaggcgtt cagggcacgc ggttttcccg tggtcttcac caccacggcc tatgacatca
     3301 ccgacggccc gaactccgac atggggctct gggggcagaa gatcccgctc gagaccctct
     3361 cggccgattc cgagtgggtg gcgatcgacg accggatcgc accgcagccg ggcgagctcg
     3421 tcatcgtcaa gaagaaggcc agcgccttcc acggcacgca tctcgcgagc tacctcaccg
     3481 ccaatcgggt cgacacggtg atcgtcaccg gggtcaccgc ctgcgcctgc gtgcggcata
     3541 ccatcgagga cgcgatcgcc gacgggttca ggccgatcgc cgttcgcgag gggatcggcg
     3601 accggatcgc cggagcggtg gagtggaacc ttttcgacat cgatgcgaag ttcggggatg
     3661 tcgagccgct cgagcgtgtg ctccaatacc tccagtccaa cgagatcggc tgagccggcc
     3721 gcccggcagc ctccacaagg gaaaagcctc atgatcagag tgggcgtgga tgtcggcggg
     3781 acgttcacgg atatcgtgct cgagcgcacc cgggcggccg gcgaggacca gcagatcgtc
     3841 gtccacaagg tgccctcgac gccgcaggac cagtccgagg gcgtcgtcaa aggggtcgtc
     3901 gagatctgcc agatggccgg catcgcgccc ggcgagatcg acatggtctt ccacggcacg
     3961 acggtcgcca ccaacatggt gatcgagcgc aagggtgccg aggtgggcat gatcaccacc
     4021 cgcggctttc gcgacatcct gcacatggcc cgccacaagc ggccgcacaa tttctcgctg
     4081 cagttcgacg tgccctggca gtcgaagccg ctggtcaagc ggcgcaaccg cctgccggtg
     4141 accgagcgga tcatgccgcc ggacggccgg gtggaggtgc cgctcgacac ggacgaggtg
     4201 aaggcggcgg ccgagctctt cgccaagcgc ggcctcgacg cggtgatcgt gggcttcctc
     4261 ttcgccttcc tcaacgacga gcacgagcag aaggccaagg cgatcgtcga ggcggccatg
     4321 ccggacgcct atgtctgctg ctcgtcggag atcgtgaacg tgatccgcga gtacgagcgg
     4381 ttctcgacct gtgccatgaa cgcctatatc gggccgatga ccgcgcgcta catgcgccag
     4441 ctcgagggcc ggctgcgcgc ggccggggtc cgggccgcgg tccgcatcat gcagtcgaat
     4501 ggcggcatct cgaccgtcga gaacgccatc cagcggccgg tcggcctctt gctctccggc
     4561 ccggccggcg gcgtcatcgg cggccgctgg accggcgagc atgccggcac gcagaacgtc
     4621 atcaccatcg acatcggcgg cacctcggcc gacatcagcg tcatccaggg cggcgagctc
     4681 cggatcaaga acccgcgcga caccgaggtc gccagcctgc cggtgctcgt gccgatgatc
     4741 gacgtcgacg cgatcggcgc cggcggcggc tcgatcgcct atctcgatcc gggcggcgcc
     4801 ttccgcgtcg gcccgcgctc ggccggcgcc gaccccggcc ccgcctgcta tgacaagggc
     4861 ggcgacgagc cgaccgtcac cgacgcccag gtggtgctcg gccggctcga ccccgagcag
     4921 ttcctcggtg gcgacctcgc catcgagccg tcgctgtcgg agcgtgcgat agccaagcac
     4981 atcgccgggc cgatggggct ttcggtcacc gaggcggcgc tcggcatcct gcgcatcatc
     5041 aacaacaaca tggcgctcgc catccacaac gcctcggtcg ccaagggcat cgatccccgc
     5101 aacttcacgc tgatgggctt cggcggcgcc ggcccgctgc acgccaccgc cctggccgag
     5161 ctgatctccg cccgggacgt gatcgcgccc ctgcagcccg gcatcacggc ggccatgggc
     5221 ctgctcgtga ccgacctgca gtacgagtac acgcattcgg tgctgctggt cctgaaccag
     5281 gcgggcgagg cggagttcgc ggccctcaac gccgccatgg acgagctgcg cgcccaggct
     5341 gaagcgcagc tcgaggccga cggcatcgcc cccggcgccc ggcgctttcg caaggtcgcc
     5401 gagtgccgct atctggggca gggcttcgag cttcgggccg acatgccgga ggggccgctg
     5461 gccaaggaca atgtccagga ggtcatcaga agcttcttcg acgcgcacca gcaggtctac
     5521 ggccatgcct tcgaggacca gctcaccgag gcggtgacgc ttcggctgat cgccacggcc
     5581 gaggtcgaca tgctgcgcct gcccgatctg gcgcagggtg gcaggaccga cccaaaggac
     5641 gccgtgctct acgtccgcga caccgtcttc gacgacggcg aggcgcgacg gacgccgcgc
     5701 taccggcgcg atcggctgct cgccggcgac cgggtgacgg ggccggcgct catcgtccag
     5761 cacaactcga cgacgctcct gccgccgggc tacgcggccg aggtgctcgg tcacggcgac
     5821 atccgtgtcg ccaagcgcgc ctgaggagaa aaacatgacc agggaagtcg atccgatcac
     5881 gctgcaggtc atcggcggcg ccttgcacgc catcgccgag cagatgggca acgtcctcta
     5941 ccggatgagc tattcgtcca tcatccggga gagccaggat ctgggggcag ggctgttcga
     6001 ccgggacttc aacacgctct gcgaatccga ttcgacgccg atgcatatcg gctcgctgcc
     6061 gggctatctc aggggcatcg cccggaccat tccgatcgac ggctggcagc ccggcgactg
     6121 cgtcatccac aaccacccct atgccggggc gagccactcg cccgacatcg ccatcgtcat
     6181 gccggtcttc ttcgagggcg agctgatcgg cttctcggcc aacacggcgc accatgtcga
     6241 catcggggcc gccacccccg gcctgatcat cgacgtgccc gacatgtacg ccgaaggcat
     6301 gctgatggac ggcctcaagc tcttcgaggc cggcaagcgc aacgaggccc tgtggaagtt
     6361 catctccgac aacacgcgcg tccccagcct ggtgaagggc gacctcgagg cgcagatcgc
     6421 ctcggcggag ctgggcgtgc ggcgcttcca ggagctgctc gccacctacg gcagggacca
     6481 ggtgatccag gcgacccggc agctcatgga ctacaccgag agcatgctca ggcgcgagat
     6541 cgcgaagatc ccggacggcg actacacggc cgagggcttc ctcgacgacg acgggcgcaa
     6601 tcggggcaag catctgccga tcaaggtgac ggtcaaggtc cggggcgacg gggtcgaggt
     6661 cgatctcacg ggctcctccg accaggtgcc gaccgccttc aacgtgccgt tcgagggctc
     6721 gaccaacgtc gcctgcttct tcgccttccg tgccttgctg ctcgacacct acacgcacga
     6781 ggagcacatc ccgcagaacg agggctcgtt ccgcccggtc aaggtcacgg caccgctcgg
     6841 ctcgatctac aacccgatcg ccccggtcgc ggcggaggct cggttctgcc agatccagcg
     6901 cgtcgtcgac ctgatcatca aggcgctggc cccggtgatc ccggagaagg ccacggccgg
     6961 caacgccgcg acgctctcct tcgccgcctt ctcgggcgtc aggccgtccg gcgactactg
     7021 ggtcttcctc gaggtcaacg aggcggcgat gggcggccgg cccacctcgg acgggccgga
     7081 taccgtcgag gagctgatgc gcaacacgcg caacaacccg ctcgaggacc tgggcatgca
     7141 cctgccgctc atctgcgacc gctacgaggt gcgcgacgac gtggcgccgg gggcggggag
     7201 gttccgcggc ggggcggggg tggtgaaggc gcagcgctac ctgacgccgg gcttcatgac
     7261 gcacgaatcg gaccgccacg acgatgcgcc ctggggcatc ttcggcggca agcagggtgc
     7321 cgtcggcaag atggagatct acaacgtggc ccggccggac gagatcgcca gcgagcccgc
     7381 gaagttctcg ggccggcgga cacagccggg cgacgtggtc gcctatttct cgccttcggg
     7441 cggcggctat ggcgatccgc tcgagcgtga cccggcgaag gtgctggacg acgtgctcga
     7501 cggcttcatc accgtgcagc acgcgctcga cgactacggc gtggtgctcg atgcggtcga
     7561 cgacggctat ggttggcagg tcgacgccga ggcgacctgg gcccgccgca acgagatgcg
     7621 cgccggctga acgcgccggg cgggatgcgc cctcggctgc gcaccagcct gccgggtctt
     7681 ggatgcatct tgtgcaagtg ggcaaacccg gtccacaatt tgcccgggtg atccgcaagg
     7741 gagaggacac gatgaccgag aagaagctcg aagacgccac gcccccggcc gccgcgggca
     7801 gcggcctcag ccgccgcagc atcctcaagg gcacggccgc cgccaccggc ctcgccgtcg
     7861 gctccggggc catcaccggc ttcccgacca tctgggcgca gaacatcaag gacatcaccc
     7921 tcaaccacac cggcatgtcc tattcgaccc tcatcgacat cgcccggcag gcgtcggccg
     7981 atctcggctt caccgtcgag atgtcggtga ccgaccatcc gggcctgacc aaccggatga
     8041 tcaacgaccc gggctcgatc gacatcgccg acgcggagat ctggcaggcc aaggtgttcg
     8101 tgccgcaggg cgtgacccag gccgtcgacg tcaagcgcat caacctctgg gacgagttca
     8161 cgccgatcta caccaagggc gagttcctcg gccggccggt ctcgcgggcc ggcgattcgc
     8221 ccttcgagtt catgtatctg gacgccatcg acggcacgac cttcgtcgag gggccgaccg
     8281 agttcgccac cttcatgccc ggcgtcttca acgccgacac gctcggcatc cgcgccgacc
     8341 tggtcggccg gccgatcgag aagtggggcg acctcttctc cgaggagttc aagggcaagg
     8401 cggcgatcca gaacatcccg accatcggca tcatggacgc catcatggcc tgcgaatcgg
     8461 cgggcatcgt cacctatggc gacaagggca acccgacccg cgaggagctg gaggcgacca
     8521 tcgccaagct catcgagctg aagcgcgccg gccactggcg ggccttgtgg aacaccttcg
     8581 acgaatcggt gaacctcatg gcctcgggcg aggtggtgat ccagtcgatg tggtcgccgg
     8641 cggtgaccgc ggtgcgcacc cagggcatcg actgcgtcta ccagccgctc aaggagggct
     8701 atcgcggctg gggcaacggc ctggcgctcc tgtcccatct cgagggcatc aagctcgacg
     8761 ccgcctacga gtacctgaac tggtacgtct cgggctggac cggcggcttc atcgccaagc
     8821 agggctacta catctcggtg cccgagacgg cgaaggacca cctgaccgag aacgagtggg
     8881 gcttcttcta cctcggcaag cccgccaccg cggacatcgt cagcccgacc ggcgtcaaga
     8941 ccaactcggc cggcgacgtg cgcgacggcg gctc
//
LOCUS       DAOTFI010000204         8924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9352_length_8924_cov_6.504905, whole genome shotgun sequence.
ACCESSION   DAOTFI010000204 DAOTFI010000000
VERSION     DAOTFI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9352_length_8924_cov_6.504905"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="P5558_18115"
     CDS             <1..743
                     /locus_tag="P5558_18115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY26292.1"
                     /translation="TELLIIESIRDLSKDVQLSHEHKLSELGMLAAGVAHEIYNPLTS
                     IRLALRSLQTTPGRSTAGETDYLALIDEEIEKCIEITYRLLKLSMPKSRTPTLVELNQ
                     LVAEVVSLLQGEAESRNVAIELDLADNLRIVGSDSEMRMLVLNLVQNAFHAMPGGGRL
                     AISGRIEAGEVVLAVKDDGVGIPEADLERIFHPFWSRRADRDGHAGTGLGLSICHNIA
                     TSHDGRIGVRSEVGEGSVFTVRLPLARP"
     gene            740..2101
                     /locus_tag="P5558_18120"
     CDS             740..2101
                     /locus_tag="P5558_18120"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY26293.1"
                     /translation="MNALQGNDRSILVIEDDRTLARLIVDTLARVGFATACAHSWGEA
                     REALRRHSPALALLDMRLPDAEGLDCIEELAEHCPVIVLTAYGTIHDAVRAMRKGAVE
                     YLTKPLDADKLELAITRALQVMSLRRSYEFCRAQLHPAIGQIMIGRSPAFQEVLRQVE
                     SVADTDRTVLIQGESGVGKELVAQSLHQLSSRASHNFVAVDCSTLQGNLFESELFGHE
                     RGAFTGAERRKEGLIEVAEHGTAFFDEIGELPAALQPKMLRLLEANRFRRVGGTVDHK
                     TDARFVAASNRDLAEISKEGHFRRDLYYRLAAFVIHVPPLRARRDDIPLIAEHFLEKR
                     NFLRQTQKHFGAAAIECLVDYPWPGNIRELRNIVERAILVSGSAPEIQPRHLALDRAA
                     AGSGDSEHLLDGEPTLEEVKRMYVAQLVESHKGHRAKIAEILGISERNTYRLLKRYGL
                     SER"
     gene            complement(2098..3294)
                     /locus_tag="P5558_18125"
     CDS             complement(2098..3294)
                     /locus_tag="P5558_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26294.1"
                     /translation="MHIQDCEVFVVGNPPPGFGGRYFIFVKLTTNDGIVGWGEVYCAT
                     FGPHVVATMIEDTAARFVLGADPSSIETVWRRVYSAGYGQRPDITLVAILSGLEMAMW
                     DITGKAAGKPVWALTGGRVHERLRAYTYLYPRPGEGADFYHDADRSAEAAATAVAQGF
                     TAIKFDPAGAYSAFDPRQPSRIQLERSERFCRRLRDAVGDRADLLFGTHGQFTASGAI
                     RMAKRLEPCDPLWFEEPTPPELPEEMAKVARATSIPIATGERLATKYELARVLALGAA
                     SIIQVNLGRCGGLLEAKKIAGLAEAHYAQIAPHLYCGPVVGAANIQLATTCPNFLILE
                     SIQDWGGFHGEILEKPIRFEAGWVVPPTEPGLGVAVNEAVARAHPYTGDELHLEMAAD
                     PVLEFP"
     gene            3367..3672
                     /locus_tag="P5558_18130"
     CDS             3367..3672
                     /locus_tag="P5558_18130"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY26295.1"
                     /translation="MTVTATNDQRLGRVVAILKRFPEVRRVILFGSWAIGDWDERSDI
                     DIVVSATGIDFRRWAVIAAVVDEARTLLRIDLVWLEDASEAFALENGRTGRVIFERA"
     gene            3986..5065
                     /locus_tag="P5558_18135"
     CDS             3986..5065
                     /locus_tag="P5558_18135"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HRY26296.1"
                     /translation="MLAAVCRAFGAPLVVEEVTLAAPGPGEVEVRLGACAICHSDIHF
                     IDGAWGGALPAVFGHEAAGVVAAVGPGVAGLAPGDHVVVTLIRACGGCFYCARGELVA
                     CEADFALDARSPLADAGGVALHQGMRTGAFAERVVVERSQVVRIDAGIPLDVASLLAC
                     GVITGVGAVTNTAAVPPGASVVVIGCGGVGLNTVQGARIVGAERIIALDPVAEKRDAA
                     LAFGATDALDPLANDPGPAVRALTGGRGADYVFVAVGAKAAIEQGLGLLRKGGSLVIV
                     GMPASGVTVDLDPTTLAACGQRVLGSKMGSARIAVDIPYLVGLYRQGRLELDALVSGR
                     YPLAGINDAIASSRRGRALRNVIVF"
     gene            5092..5748
                     /locus_tag="P5558_18140"
     CDS             5092..5748
                     /locus_tag="P5558_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY26297.1"
                     /translation="MKGTIVPVTPLMQNCSVFWDDEAKLATVIDPGGDAPRIMELVET
                     QGLIVEQILLTHGHFDHAGGVVALNRLVAEKQGAAVPVRGPHEKDAFLLEALARDGAA
                     YGIPEAQPFTPDEWLDEGGTARIAGRDFAIYHCPGHSPGSLVYVDAEARFAIVGDVLF
                     QGSIGRTDFPYGDHDALIAAIRTKLFPLGDDISFICGHGPGSSFGVERRSNPYAGDGA
                     "
     gene            complement(5727..6473)
                     /locus_tag="P5558_18145"
     CDS             complement(5727..6473)
                     /locus_tag="P5558_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26298.1"
                     /translation="MTTATSTTLTTARTVIVILTGDADWQEPLDRAARLAAATGKALR
                     VLLADDDALVSAAALDCVRLVAWGGLVGAFDPTAARRLVRARRARLRGVLQGLAARHG
                     IAAELAESAPATHWAGCAALTFFGRRRRGTLLVVHAGTVDTLDVAARLALEQHQEVRL
                     LLVGAGSPAEAATELRRRFGHLLRQPPEALEEGHPLRRPSGTTRVTTVVLDPAYLTAR
                     NLTLEDLLDELRTVMGEVREATTPQAPSPA"
     gene            complement(6470..8905)
                     /locus_tag="P5558_18150"
     CDS             complement(6470..8905)
                     /locus_tag="P5558_18150"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY26299.1"
                     /translation="MVEGAPQALKVDALRRRVDPVDFADTGQGELDGLIGHERAKEAI
                     ELALALPMAGGHLFLMGNRGTGRRTTLERALERVAASRPPGTDLCYVSDFQEPRCPRA
                     LQLRPGLGRRLAADMERLVEDLADALKAAFQNEEHRTRRQLIEEEFKERQEEAVEAVE
                     KAARGQGIALLRAPTGFLFAPMREGRVIEPEAFQQLAEEERKAIAAKIEELQKQLQAA
                     LAAAPSWMQETRAKLRALNDETAASAVRHPIARLREAYAGEPTITAYLDAVEADVRGQ
                     IGVFLGYAEAHEQQPHSASVEELPPPFRRYRVNLLIDRDGATEAPVCYEDQPIFERLL
                     GRIEQRAEMGTLTTDFLMIRPGALHCANGGFLYLDAQKLLQRPLAYEGLKRALLAREL
                     RIESPLAAMGVLATQTLEPDPVPLDVKVALLGDRQLYYLLSQLDPEFPELFTIVADFD
                     DEIETADAGLLAFGRQVAGLAAAHGFRPLEPAAIAACLEDALRRAGDVERLSADIDSI
                     VDLLREADHIAGLEGAETIRAEHVHAAIGARERRLARLRDRTLERIDEGTVSIATDGE
                     EVGVINGLAVLQLGSFAFGRPSRITATVRMGAGRVVDIEREAKLGGPLHAKGVLILQG
                     YLAATFAPDFPLALSASLVFEQSYGGVDGDSASSTELYALLSALAEAPIRQGLAVTGS
                     VNQKGEVQAIGGVNEKIEGFFDVCAARGLTGRQGVLIPATNTRHLMLAPRVLEAVAAG
                     RFAVYPVRRIEEGIALLTGTEVGSRGADGHFPEGSLYGRIESRLRAFAEAQRRFGKAG
                     DKDEKPGTTEDDA"
BASE COUNT         1281 a         2959 c         3328 g         1356 t
ORIGIN      
        1 gaacggagct gctgatcatc gaatcgatcc gcgacctgag caaggacgtc cagctgtcgc
       61 acgagcacaa gctctccgag ctcggcatgc tggcggccgg ggtcgcccac gagatctaca
      121 acccgctgac ctcgatccgt ctcgccctgc gctcgctgca gacgacgccg gggcgatcga
      181 ccgcgggcga gaccgactat ctggcgctga tcgacgagga gatcgagaag tgcatcgaga
      241 tcacctatcg gctgctgaag ctcagcatgc ccaagtcgcg gacaccgacg ctggtcgagc
      301 tgaaccagct cgtggccgag gtcgtgtcgc tcctgcaggg cgaggccgaa agccgcaatg
      361 tggcgatcga gctcgatctc gccgataatc tgcggatcgt cggcagcgac agtgagatgc
      421 gcatgctcgt gctgaacctc gtgcagaacg ccttccatgc catgcccggc ggtggccggc
      481 tggccatcag cggccggatc gaggcggggg aggtcgtgct ggccgtcaag gacgacggcg
      541 tgggcatccc cgaggccgat ctcgagcgca tcttccaccc gttctggagc cggcgcgccg
      601 accgtgacgg ccatgccggc accgggctcg gcctgagcat ctgccacaac atcgcaacca
      661 gccacgacgg acggatcggc gtgcgctcgg aggtcggcga gggcagcgtc ttcaccgtgc
      721 ggctgccgct ggcccgcccg tgaacgcgct ccagggcaac gaccgctcga tcctggtgat
      781 cgaggacgac cggactctcg cccggctgat cgtcgacacg ctggcgcgcg tcgggttcgc
      841 cacggcctgc gcccatagct ggggcgaggc ccgggaggcg ctgcgccgcc acagcccggc
      901 cctggccctg ctcgacatgc gcctgccgga cgccgaaggt ctcgactgca tcgaggagct
      961 ggccgagcat tgcccggtca tcgtgctgac cgcctacggc accatccacg atgccgtcag
     1021 ggcgatgcgc aagggggccg tcgagtacct caccaagccg ctcgatgccg acaagctgga
     1081 gctggcgatc acccgggcgc tgcaggtgat gtcgctgcgg cgcagctacg agttctgccg
     1141 ggcgcagctg catccggcga tcggccagat catgatcggc cgcagcccgg ctttccagga
     1201 ggtgctgcgg caggtggaat cggtcgccga caccgatcgc acggtcctca tccagggcga
     1261 gagcggtgtc ggcaaggagc tggtggccca gagcctgcat cagctcagct cgcgcgccag
     1321 ccacaacttc gtggccgtcg actgctccac cctgcagggc aatctcttcg agtccgagct
     1381 gttcggccac gagcggggcg ccttcaccgg cgccgagcgg cgcaaggagg ggctgatcga
     1441 ggtcgccgag cacggcacgg cgttcttcga cgagatcggc gagctgccgg ccgccctgca
     1501 gcccaagatg ctgcggctgc tggaggccaa ccggttccgc cgggtcggcg gcacggtcga
     1561 tcacaagacc gacgcgcggt tcgtcgccgc cagcaatcgt gacctcgccg agatcagcaa
     1621 ggaaggccac ttccgccgcg atctctatta tcgcctggcc gccttcgtca tacatgtgcc
     1681 gccgctgcgc gcccgtcgcg acgacatccc tctgatcgcc gagcatttcc tcgagaagcg
     1741 caacttcctg cgccagacgc agaagcactt cggcgcggcg gccatcgagt gcctcgtcga
     1801 ctatccgtgg ccgggcaata tccgcgagct gcgcaacatc gtcgagcgcg ccatcctggt
     1861 ctcgggcagc gccccggaga tccagccgcg ccacctcgct ctggatcggg cggcggccgg
     1921 gagcggcgac agcgagcatc tgctcgacgg ggagccgacc ctggaggagg tcaagcgcat
     1981 gtacgtcgcg caactggtgg agagccacaa gggccaccgc gccaagatcg ccgagatcct
     2041 gggcatcagc gagcgcaaca cctatcgcct gctcaagcgc tacggcctgt cggagcgtta
     2101 ggggaattcc agcaccgggt cggcggccat ctcgagatgg agctcgtccc cggtataggg
     2161 atgggcgcgg gcgacggcct cgttgacggc gacgccgagg ccgggctcgg tcggcgggac
     2221 gacccagccg gcctcgaagc ggatcggctt ttccaggatc tcgccgtgga agccgcccca
     2281 gtcctggatg ctctcgagga tcaggaagtt cgggcaggtg gtggcgagct ggatgttggc
     2341 ggcacccacc accgggccgc aatagaggtg cggggcgatc tgggcgtagt gggcctcggc
     2401 gaggccggcg atcttcttgg cttcgaggag cccgccgcag cggccgagat tgacctggat
     2461 gatcgaggcg gcgccgaggg cgaggacgcg ggcgagctcg tacttggtgg cgagccgctc
     2521 gcccgtggcg atcgggatcg aggtggcgcg ggcaaccttc gccatctcct cgggcagttc
     2581 cggcggggtc ggctcctcga accagagggg atcgcagggc tcgagccttt tcgccatgcg
     2641 gatggcgccg gaggcggtga actggccgtg cgtgccgaag aggagatcgg cccggtcgcc
     2701 gaccgcgtcg cgcagccgcc ggcagaagcg ctcggaacgc tcgagctgga tccgcgacgg
     2761 ctggcgcggg tcgaaggcgg aataggcgcc ggccggatcg aacttgatgg cggtgaagcc
     2821 ctgggccacg gccgtggcgg cggcctcggc cgagcggtcg gcgtcgtggt agaaatccgc
     2881 gccctcgccg gggcgcgggt agagataggt gtaggcgcgc agccgctcat ggacccgccc
     2941 acccgtcagc gcccagacgg gcttgcccgc ggccttgccg gtgatgtccc acatcgccat
     3001 ctcgaggccc gagaggatgg cgacgagggt gatgtcgggg cgctggccgt agccggccga
     3061 gtagacccgg cgccagaccg tctcgatcga ggagggatcg gcgccgagga cgaagcgggc
     3121 cgccgtgtcc tcgatcatgg tggccacgac atgcgggccg aaggtggcgc agtagacctc
     3181 gccccagccc acgatgccgt cattggtggt gagcttgacg aagatgaagt agcggccgcc
     3241 gaagccgggc ggcgggttgc cgacgacgaa gacctcgcag tcctggatat gcatgtcggt
     3301 cactcctccg cctgctcccc gacaacgggc tttccagtcg ccgcggttgc ggccatgctc
     3361 tagcgcatga cggtaaccgc gaccaacgac caacggctgg gccgggtcgt cgccatcctc
     3421 aagaggtttc ccgaggtgcg ccgggtgatc ctctttggtt cgtgggcgat cggcgattgg
     3481 gacgagcgct cggacatcga cattgtcgtc tcggcaaccg ggatcgactt ccgacgctgg
     3541 gccgtgatcg cggccgtagt ggacgaggcg cggacgttgc tgcggattga cctcgtgtgg
     3601 ctcgaggatg cgagcgaggc gtttgcactg gagaacggac gaaccggaag ggtgatcttt
     3661 gaacgagcgt aggaagtcga aagggccgga aaaggagcga cgggcgccgt ctggttccga
     3721 ggagagttcg acgatcgccc attcgacatt ccttgcgtgg gagccgagcc tgcacgagct
     3781 tttcacatgc tcgccgctgg ccgatctcga gcttggcgag ccgggcgtgc ccatgccggt
     3841 gcgcgaggtt gtgctctgac cggctgaata ttcgatattg atggtcggac cgcgtgttgg
     3901 ccgacgcacc gcggcaatac gcaagcttcg cgttcctgcg ccggctttgc tagaggtgcg
     3961 gcgccctgaa gccgaggtga atcccatgct ggctgccgtc tgtcgggcgt ttggcgcgcc
     4021 gctcgtggtc gaggaggtga cgctggcggc gccggggccg ggggaggtcg aggtgcggct
     4081 gggggcctgt gcgatctgcc acagcgacat tcatttcatc gacggggcct ggggcggggc
     4141 gctgccggcc gtcttcgggc acgaggcggc gggggtggtg gcggccgtcg ggccgggggt
     4201 cgcgggtctg gcgccgggcg atcatgtggt ggtgacgctg atcagggcct gtggcgggtg
     4261 cttttattgc gcgcggggcg agctggtggc ctgcgaggcc gattttgcgc tggatgcgcg
     4321 ctcgcctctc gcggatgccg gcggcgtggc gctgcaccag gggatgcgga cgggcgcctt
     4381 cgccgagcgg gtggtggtcg agcggtcgca ggtggtgcgg atcgatgcgg gcatcccgct
     4441 cgacgtggcg tcgctgctcg cctgcggggt gatcaccggg gtcggggcgg tcaccaacac
     4501 ggcggccgtg ccgcccggcg ccagcgtcgt cgtgatcggt tgtggcgggg tcgggttgaa
     4561 caccgtgcag ggggcgcgga tcgtcggagc ggagcggatc atcgccctcg acccggtggc
     4621 ggagaagcgc gacgcggcgc tcgctttcgg ggcgaccgac gccctcgacc cgctggcgaa
     4681 tgacccgggc ccggcggttc gcgcgttgac cggtgggcgc ggggcggact acgtcttcgt
     4741 cgcggtcggg gccaaggcgg cgatcgagca ggggctcggc ctgctgcgca agggcggcag
     4801 cctcgtcatc gtcggcatgc cggcctcggg ggtcacggtc gatctcgacc cgacgacgct
     4861 cgccgcttgc gggcagcggg tgctcggctc gaagatgggc agtgcccgga tcgccgtcga
     4921 cattccgtat ctcgtgggcc tctatcgcca ggggcgcctc gagctcgacg ccctggtctc
     4981 cggccgctac ccgctggccg ggatcaacga cgccatcgcc tcgtcccggc gcgggcgggc
     5041 cctgcgcaac gtcatcgttt tttgaagctt tcggagaagc ccttgtcgca gatcaaagga
     5101 accatcgttc cggtgacgcc gctcatgcag aattgcagcg tcttctggga cgacgaggcg
     5161 aagctggcca ccgtcatcga cccgggcggc gacgcgccgc gcatcatgga gctggtggag
     5221 acccagggcc tgatcgtcga gcagatcctc ctcacccacg ggcatttcga ccatgccggt
     5281 ggcgtggtgg cgctgaaccg gctggttgcc gagaagcagg gtgccgcggt gccggtgcgc
     5341 gggccgcacg agaaggacgc cttcctgctc gaggcgctgg cccgggacgg ggcggcctat
     5401 ggcattcccg aggcccagcc gttcaccccc gacgaatggc tcgacgaggg cgggaccgcc
     5461 cgcatcgccg ggcgcgactt cgccatctat cactgccccg gccattcgcc cggcagcctc
     5521 gtctatgtcg atgccgaggc gcgcttcgcc attgtgggcg atgtcctctt ccagggctcg
     5581 atcggccgca ccgattttcc gtatggcgac catgacgcgc tgatcgctgc catccgcacc
     5641 aagctctttc cgctcggtga cgacatcagc ttcatctgcg ggcacgggcc gggctcgagc
     5701 ttcggggtcg agcggcggag caacccctat gccggcgacg gtgcctgagg cgtcgtcgct
     5761 tcacggacct cgcccatgac ggtgcggagc tcgtcgagga ggtcttcgag ggtcaggttc
     5821 cgggcggtca ggtaggcggg gtcgagcacc accgtcgtca cccgtgtggt gcccgacggg
     5881 cgcctgagcg ggtggccctc ttccagcgcc tcgggcggct ggcgcaggag atggccgaag
     5941 cgccggcgca gctcggttgc cgcctcggcc ggcgacccgg ctccgaccag gaggaggcgg
     6001 acctcctggt gctgctccag ggcgaggcgc gcggccacat cgagcgtgtc gacggtgccg
     6061 gcatgaacga ccaggagcgt gccgcggcgg cgccggccga agaaggtgag cgcggcgcag
     6121 ccggcccaat gggtggcggg ggcgctctcg gcgagctcgg cggcgatgcc gtggcgggcg
     6181 gcgaggccct gcagcacgcc acggagccgg gcccttcggg cgcgcacgag gcggcgcgcg
     6241 gccgtggggt cgaaggcgcc gacgaggccg ccccaggcga ccagcctgac gcagtccagg
     6301 gcggcggcgc tgaccagcgc gtcgtcgtcg gccaggagga cgcgcagtgc cttgcccgtg
     6361 gcggcggcga gccgggccgc gcggtccagg ggctcctgcc agtcggcatc acccgtgagg
     6421 atgacgatga cggtgcgcgc ggtggtcagg gtggtggagg tggccgtggt cacgcgtcgt
     6481 cctccgtcgt gcccggcttc tcgtccttgt cgccggcctt gccgaagcgg cgctgcgcct
     6541 cggcgaaggc gcgcaggcgc gactcgatgc ggccgtagag cgagccttcc gggaaatgcc
     6601 cgtcggcgcc gcggctgccg acctcggtgc cggtcaaaag ggctatcccc tcctcgatcc
     6661 gccggaccgg gtagacggcg aagcggccgg ccgccacggc ctcgaggacc cgcggtgcca
     6721 gcatcaggtg gcgggtgttg gtggccggga tcaggacacc ctgccggcct gtcaggccgc
     6781 gggccgcgca gacgtcgaag aagccctcga tcttctcgtt gaccccgcct atcgcctgga
     6841 cctcgccctt ctggttgacc gagccggtga cggcgaggcc ctggcggatg ggggcctcgg
     6901 ccaaagccga gagcagcgcg tagagctcgg tcgaggaggc gctgtcgccg tcgaccccgc
     6961 cataggactg ctcgaagacc agggaggccg agagggcgag cgggaagtcc ggggcgaagg
     7021 tggcggcgag gtagccctgg aggatgagga cacccttggc gtgcaggggg ccgccgagct
     7081 tggcctcgcg ctcgatgtcg accacccgcc cggcgcccat gcggaccgtc gcggtgatcc
     7141 ggctcggccg gccgaaggcg aagctgccga gctggaggac ggcgaggccg ttgatgacgc
     7201 cgacctcctc gccgtcggtg gcgatgctga ccgtgccttc gtcgatccgc tcgagcgtgc
     7261 ggtcgcgcag ccgggcgagg cgccgctcac gcgccccgat ggcggcgtgg acgtgctcgg
     7321 cccggatggt ctcggcgcct tcgaggccgg cgatatggtc ggcctcgcgc agcaggtcga
     7381 cgatgctgtc gatgtccgcc gagagccgct cgacgtcgcc cgcccggcgc agcgcgtcct
     7441 cgaggcaggc ggcgatggcg gcgggctcga gcgggcgaaa gccatgggcg gcggcgagcc
     7501 cggcgacctg ccggccaaac gccagcagcc ccgcatcggc ggtctcgatc tcgtcgtcga
     7561 agtcggcgac gatcgtgaag agctccggga actcgggatc gagctggctc aggaggtagt
     7621 agagctggcg gtcgccgagc agcgccacct tgacgtcgag cggcaccgga tccggctcga
     7681 gcgtctgggt ggcgaggaca cccatggcgg ccaggggcga ttcgatccga agctcccggg
     7741 cgagcaaggc ccgcttgagg ccctcgtagg cgagcgggcg ctgcagcagc ttttgcgcat
     7801 cgagatagag aaagccgcca ttggcgcagt gcagggcacc cggccggatc atcaggaagt
     7861 cggtggtcag cgtgcccatc tcggcccgct gctcgatccg cccgagcagg cgctcgaaga
     7921 tcggctggtc ctcgtagcag acgggggcct cggtggcgcc gtcgcggtcg atcaggaggt
     7981 tgacccgata gcggcggaag ggcggcggca gctcctcgac gctggccgag tgcggctgct
     8041 gctcgtgggc ttcggcatag ccgaggaaga cgccgatctg gccgcgcacg tcggcctcga
     8101 cggcgtcgag ataggcggtg atggtcggct ccccggcata ggcctcgcgc agccgggcga
     8161 tcgggtggcg gaccgccgag gcggcggtct cgtcgttgag ggccctcagc ttcgcccggg
     8221 tttcctgcat ccagctcggt gccgcggcga gcgccgcctg gagctgcttc tgcagctcct
     8281 cgatcttcgc cgcgatcgcc ttgcgctctt cctccgccaa ttgctggaag gcctcgggct
     8341 cgatcacccg gccctcgcgc atgggcgcga agagaaaacc ggtgggggcg cggaggaggg
     8401 cgatgccctg gccgcgtgcc gccttctcga ccgcctcgac agcctcctcc tgacgctcct
     8461 tgaattcctc ctcgatcaac tggcgccggg tgcggtgctc ctcgttctgg aaggcagcct
     8521 tgagcgcgtc ggcgaggtcc tcgaccaggc gctccatgtc ggcggcgagc cgccggccca
     8581 gcccggggcg gagctgcagg gcacgcgggc agcgcggctc ctggaaatcg ctgacatagc
     8641 agaggtcggt gcccggcggc cggctggcgg cgactcgctc gagcgcccgc tccagggtgg
     8701 tgcggcggcc ggtgccgcga ttgcccatga ggaagagatg tccgcccgcc atgggcagcg
     8761 ccagagcgag ctcgatcgcc tccttggccc gctcgtggcc gatcaggccg tcgagctcac
     8821 cctggccggt gtcggcgaaa tcgacgggat cgacccgccg ccgcaacgcg tccaccttca
     8881 gggcctgcgg agcgccctcc accattcgcc gatcctcccg atgc
//
LOCUS       DAOTFI010000205         8910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9374_length_8910_cov_7.516996, whole genome shotgun sequence.
ACCESSION   DAOTFI010000205 DAOTFI010000000
VERSION     DAOTFI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9374_length_8910_cov_7.516996"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="P5558_18155"
     CDS             <1..510
                     /locus_tag="P5558_18155"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY26300.1"
                     /translation="GTGYLQIASILQMKGEEAGWEYLRGLDKNIAQYIKSGSRPCNSA
                     SQGEYAIGASFALRAIKNIAEGYPITMVIPAEGAGNELEANALMASSDNKEAAQRFLD
                     WTLSDRAVDTYYDWKEIVTVSGGAMPENFKAAGLPDDIGSVMWDMDFAWSAENRDRIL
                     EQWTAEFDN"
     gene            557..1609
                     /locus_tag="P5558_18160"
     CDS             557..1609
                     /locus_tag="P5558_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26301.1"
                     /translation="MTLAIRGVVKRFGALTALDAVDLDIEQGELVCFLGPSGCGKTTL
                     LRVIAGLETADAGAVLLDGADLIATPARLRNFGVVFQSYSLFPNRDAGENIAYGLECR
                     RWAKDRIRARVAEMLQLIGLADHAGKLPSQLSGGQQQRVALARALAVDPALLLLDEPL
                     SALDAKVREGLRFEIRALQQRLGITTIMVTHDQEEALTMADRIVVMRAGRIEQVGTAA
                     ELYDHPETRFVAEFVGRINVLPVAPDGGVCGRLMALPEGRLAGRRPALVGIRPEAVRV
                     HAPEAAPAGALGAQLEQAIFLGNLTQLVLRVEGAPEGDPPLLAELHGLRPDGFAPGAP
                     FAVELPPAALRVLEWR"
     gene            1600..3267
                     /locus_tag="P5558_18165"
     CDS             1600..3267
                     /locus_tag="P5558_18165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY26302.1"
                     /translation="MALSGAVARPPRHDRLPIALVTLAVAAPLLLFILYPLAFILGRS
                     FVTETGLGFANYARMLGNERFLGILANSFAVTLVSTVLAVVLAYLFAYAIQRTAIPWK
                     GFFRLVAIVPLFAPSLVQAQGLVLLFGRNGLINRTFDAGIEIYGYWGIVIAMVLYVFP
                     YAFLILSAALAVADARIYESAEMLGAGGARVFRTVTLPATRYGLAAAIFVCFTLVITD
                     FGNPMVIGADYGVLATEVYNQVIGQANFERGTVIGMILLVPAAIAAFGEKYISARQFA
                     VVGEQSRPLAIQPSPGRDAVFTAVVVAIALAILAIVAIVVFASFVDLWPYRMNLSLRH
                     YAFDVQNGIEPLWNSILIGLMAAAIGVVAITASSYMVEKLKSAASRPLYFVSILPAAV
                     PGMVLGLGYILAFNNPANPVYLIYGTLLIIAICNVYHYHAQAFLISSTSLKQISPTFD
                     EASATLGGSRFDTFRKVTLPLLAPTLVGIAVFFFMRSMVTLSAVIFLITPSTQVAAVS
                     VLLLEDRGATNQAAAFSVCIMAVVVAALLLTHMALRLAGFRNVSLIR"
     gene            complement(3313..3510)
                     /gene="ccoS"
                     /locus_tag="P5558_18170"
     CDS             complement(3313..3510)
                     /gene="ccoS"
                     /locus_tag="P5558_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRY26303.1"
                     /translation="MTYLVLVPLSIAMGLIGLGAFFWALRNDQFDDPDGAAERILTPH
                     AGPARPRQRAPGVGSRTTDRS"
     gene            complement(3507..4325)
                     /locus_tag="P5558_18175"
     CDS             complement(3507..4325)
                     /locus_tag="P5558_18175"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRY26304.1"
                     /translation="MKRRAILEPLPTLHPPLRPPSAFARHLPASAALDWLQAGWADLR
                     AAPGPSLAYGAFLFALSLAVLVVLWAAGLLYLVLPAVSGFLIVGPFLAVGLYEKSRAR
                     EAGRPLGLAQMILVRPVSGAQLAYAGLFLGLLVLFWIRAADLLYALFFGLNPFPGADE
                     AMVNVLTTPRGWALILVGSAVGAIFAAFAFAISLFSIPMLMAGQRDALTAMGTSFAMT
                     AHNLKVVLAWGVIVAIGLAFSVATFLLGLIVVFPVLGYGTWHAWRAIGGTTDRP"
     gene            complement(4348..4512)
                     /locus_tag="P5558_18180"
     CDS             complement(4348..4512)
                     /locus_tag="P5558_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26305.1"
                     /translation="MSGRWSQKRLAILAALVLAGVFVLANVHLVTVAFTSQPACVAPT
                     LDRPAAKPAC"
     gene            complement(4554..5447)
                     /gene="ccoP"
                     /locus_tag="P5558_18185"
     CDS             complement(4554..5447)
                     /gene="ccoP"
                     /locus_tag="P5558_18185"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRY26306.1"
                     /translation="MSAHDGEAPDVDPVTGYETTGHDWNGIKELNTPFPIFVITILGL
                     TVVYSIIAWLLLPAWPTGRDYTRGLLGLDQGEMAVAGYQRLADGREDWMARFVAGDFD
                     AIVADQALMAKAMPAAHRLFLDNCAACHGTDATGGPGFPDLADSDWLWGGDPATIAET
                     LRVGINSPHPETRFAQMPAFGHDQMLERAEIETLTSYVQALSTGQADPDGEGGTLFAD
                     NCASCHGDGGVGGLGIGAPSLVDGHWIYGGDRATIFATLQRGRQGVMPTWADRLSEAE
                     INLLAVYVSGLAAEHEALASR"
     gene            complement(5444..5635)
                     /locus_tag="P5558_18190"
     CDS             complement(5444..5635)
                     /locus_tag="P5558_18190"
                     /inference="COORDINATES: protein motif:HMM:NF017366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRY26307.1"
                     /translation="MMPSHETLVVLAKTFGLFWMMGFFVIVAVLAFRPSRKAAYERVA
                     LSILPGADRPLDDGPGERK"
     gene            complement(5632..6372)
                     /gene="ccoO"
                     /locus_tag="P5558_18195"
     CDS             complement(5632..6372)
                     /gene="ccoO"
                     /locus_tag="P5558_18195"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRY26308.1"
                     /translation="MLRLHYNLEKISMGLVAAIIVAASIGGIVEIAPLFTVDETVTIP
                     DDMRPHTPLELAGREIFMREGCYACHSQMIRTLADEIDRYGPYSLPSESAYDHPMLWG
                     SKRTGPDLARVGGKYSDPWHVAHLIDPRAVVPASIMPAYPWLMEPLDTGNLGAELATL
                     ARLGVPYTEEMATNAAADALAQGNPDSDGAADVATRYGEKVAIRAFDGDPARLTEMDA
                     LVAYLQSLGTLTDVAFETEEVKVPEEAP"
     gene            complement(6377..8023)
                     /gene="ccoN"
                     /locus_tag="P5558_18200"
     CDS             complement(6377..8023)
                     /gene="ccoN"
                     /locus_tag="P5558_18200"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRY26309.1"
                     /translation="MIRSLSSSERQLGIALSVALALLGLVMLGAAKVGPMAVHGAMAI
                     AVGVALLFALGGGLAEPEPRGDRLARYYDEPTRFGIVMTMIWAVFAMAVGVWVAALLY
                     WPEATPLWPWTSFGRLRPVHTSGIIFGFGGNALIATSFHVVQRTSRARLADSVTPWVV
                     LIGFNLFCLWAVSGYLMGSTQSKEYAEAEWYADLWLVVVWVVYFVLYMRTLARRNEPH
                     IYVANWYYLAFILVVAMLHIVNNLALPVSLAGAKSFTIWAGVQDAMVQWWYGHNAVAF
                     FLTAGFLAMLYYFLPVRSGRPIWSYRLSILSFWGIVFFYVWVGSHHLHYTALPYWVQT
                     LGMTFSVMLLVPSWAAAGNAIATLNGAWHKVRDDATLRFMFVAAVFYGLSTFEGSFLA
                     IRPVNSLSHYTDWTVGHVHSGTLGWVAMIIFGAIYTLAPQLSGRETMAWPRAIEWHFW
                     LAVTGTLVYVVALWNAGITQGLMWRTYDASGALSYSFLQSVMAMAPYYLLRTIGGALF
                     LAGAVICALNTWATMRSARAGAEAHDRPLLPGPALPEPAE"
BASE COUNT         1411 a         2980 c         3156 g         1363 t
ORIGIN      
        1 ggcacgggct acctgcagat cgcctcgatc ctgcagatga agggcgagga ggcgggctgg
       61 gagtacctgc gcggcctcga caagaacatc gcccagtaca tcaagagcgg gtcccggccg
      121 tgcaacagcg ccagccaggg cgagtacgcc atcggcgcgt catttgcctt gcgggcgatc
      181 aagaacatcg ccgagggcta cccgatcacc atggtgatcc cggccgaggg tgccggcaac
      241 gagctcgagg cgaatgcgtt gatggcgagc tcggacaaca aggaggcggc ccagcggttc
      301 ctcgactgga cactctccga ccgtgcggtc gacacgtatt acgactggaa ggagatcgtc
      361 accgtctccg gcggtgccat gcccgagaac ttcaaggcgg ccgggttgcc ggacgacatc
      421 ggctcggtga tgtgggacat ggacttcgcc tggtcggcgg agaaccgcga ccgcatcctc
      481 gagcagtgga cggccgagtt cgacaactga gccggttgcg gcagagcatg gcgggacgga
      541 tcgaggcggg cgtccagtga cgctcgcgat ccggggggtg gtcaagcgct tcggtgccct
      601 gaccgccctc gacgccgtcg acctcgacat cgagcagggc gagctcgtct gcttcctcgg
      661 cccctcgggc tgcggcaaga ccaccctgct ccgcgtcatc gccgggctcg agacggccga
      721 tgcaggggcc gtgctgctgg acggcgccga cctcatcgcc acgccggcca ggctgcggaa
      781 cttcggtgtc gtcttccagt cctactcgct cttccccaac cgcgacgcgg gggagaacat
      841 cgcctacggg ctcgaatgcc ggcgctgggc caaagaccgg atcagggcgc gcgtcgccga
      901 gatgctgcag ctgatcggcc tcgcggacca tgcgggcaag ctgccgtccc agctctcggg
      961 cggccagcag cagcgcgtgg cgctggcccg ggcacttgcg gtcgatccgg cgctgctgct
     1021 gctcgacgag ccgctctccg ccctcgacgc caaggtccgc gaagggctgc ggttcgagat
     1081 cagggcactg cagcagcgcc tcggcatcac caccatcatg gtgacgcacg accaggagga
     1141 ggcgctcacc atggccgacc gcatcgtcgt gatgcgggcc ggcaggatcg agcaggtggg
     1201 cacggcggcg gagctctacg accacccgga gacccgcttc gtcgccgagt tcgtcggccg
     1261 catcaacgtc ctgcccgtgg cccctgacgg cggggtctgc ggccggctca tggcgttgcc
     1321 tgaaggaaga ttggcagggc gccggccggc gctggtcggc attcgccccg aggcggtccg
     1381 ggtccacgcc cccgaggcgg ccccggccgg ggcgctcggg gcgcaactgg agcaggcgat
     1441 cttcctcggc aatctcacgc agctcgtgct gcgcgtcgag ggtgcaccgg agggcgatcc
     1501 gccgctcctg gccgagctgc acgggctcag gccggatggc ttcgccccgg gggcaccgtt
     1561 cgccgtcgag ctgccgccgg cagcacttcg ggtgctcgaa tggcgctgag cggtgccgtg
     1621 gcccgcccgc cgcggcacga ccggctgccg atcgcgctgg tgacgctcgc ggtcgccgcc
     1681 ccgctcctgc tcttcatcct ctacccgctc gccttcatcc tcgggcgcag cttcgtcacc
     1741 gaaaccgggc tcggcttcgc caactacgcg cggatgctgg gcaatgagcg attcctcggg
     1801 attctcgcga actcgttcgc ggtgaccctg gtcagcaccg tcctggccgt ggtcctggcc
     1861 tacctcttcg cctacgccat ccagcggacg gcgatcccgt ggaaaggctt ctttcgcctc
     1921 gtcgccatcg tgccgctctt cgcgccgtcg ctggtccagg cgcagggcct cgtcctgctg
     1981 ttcggcagga acggcctgat caaccgcacc ttcgacgccg gtatcgagat ctacggctac
     2041 tggggcatcg tgatcgccat ggtgctctac gtcttccctt acgccttcct gatcctctcg
     2101 gccgccctcg ccgtggccga tgcgcggatc tacgaatcgg ccgagatgct gggggccgga
     2161 ggggcccgcg tctttcgcac ggtgaccctg cccgcgaccc gctacgggct ggcggcggcg
     2221 atcttcgtct gcttcaccct ggtcatcacc gacttcggca atcccatggt gatcggcgcc
     2281 gattacggcg tgctggcgac cgaggtctac aaccaggtca tcggccaggc gaacttcgag
     2341 cgcggcacgg tgatcggcat gatcctgctg gtgcccgccg ccatcgccgc cttcggcgag
     2401 aaatacatca gcgcccgcca gttcgccgtg gtcggcgagc agtcgcgccc gctggccatc
     2461 cagccgagcc cgggccgcga cgccgtcttc accgctgtcg tggtggcgat cgccctggcg
     2521 atcctcgcca ttgtcgccat cgtcgtcttc gccagcttcg tcgatctctg gccctaccgg
     2581 atgaacctca gccttcggca ctacgccttc gacgtgcaga acggcatcga gcccttgtgg
     2641 aactcgatcc tgatcggcct catggcggcc gccatcggcg tggtggccat caccgcctcg
     2701 tcctatatgg tcgagaagct gaaatcggcc gcctcgcgcc cgctctactt cgtctcgatc
     2761 ctgcccgccg ccgtgcccgg catggtgctg ggcctcggct acattctcgc cttcaacaac
     2821 cccgccaacc ccgtctacct gatctacggc acgctcctga tcatcgcgat ctgcaacgtc
     2881 taccattacc acgcccaggc tttcctcatc tcctcgacca gcctgaagca gatcagcccg
     2941 accttcgacg aggcgtcggc gacgttgggc ggcagtcgct tcgacacctt ccgcaaggtc
     3001 accctgccgc tcctggcacc caccctggtc ggcatcgccg tcttcttctt catgcgctcg
     3061 atggtgaccc tctcggccgt gatcttcctc atcacgccgt cgacccaggt ggccgccgtc
     3121 tcggtcctgc tgctcgagga ccggggggcg accaaccagg ccgccgcctt ctcggtctgc
     3181 atcatggcgg tcgtggtcgc cgcgctcctg ctgacgcaca tggcgctgcg cctagccggc
     3241 tttcgcaatg tctcgctgat tcgctgaggg gcgacccgca aggtgacgcg gtcgcccgac
     3301 gctgcggccg cttcagcttc ggtcggtggt gcgcgagccg acgccaggcg cccgctggcg
     3361 tgggcgggcg ggtccggcat gcggggtcag gatgcgttcg gcggcgccat cgggatcgtc
     3421 gaactggtcg ttcctgaggg cccagaagaa ggcgccgagg ccgatgaggc ccatggcgat
     3481 cgagagcggc accagcacca gataggtcat ggccggtcgg tcgtgccgcc gatggcgcgc
     3541 caggcgtgcc aggtgccgta gccgagcacc gggaagacga cgatcaggcc gaggaggaag
     3601 gtcgccaccg agaaggcgag gccgatcgcg acgatgacgc cccaggccag caccaccttc
     3661 aggttgtggg cggtcatggc gaagctcgtg cccatcgcgg tcagggcgtc gcgctgcccg
     3721 gccatcagca tcggaatgga gaacaggctg atggcgaagg cgaaggcggc gaagatggcg
     3781 ccaacggcgc tgccgaccag gatcagcgcc cagccgcgcg gcgtggtcag gacgttgacc
     3841 atcgcctcgt cggcgccggg gaagggattg aggccgaaga agagggcgta gagcagatcg
     3901 gcggccctga tccagaacag caccaggagc cccaggaaca gcccggcata ggcgagctgc
     3961 gcgccggaga cgggacgcac caggatcatc tgggcgaggc cgagcgggcg acccgcctcg
     4021 cgcgcccggc tcttctcgta gaggccgacc gcgaggaacg gcccgacgat caggaagccg
     4081 gacaccgccg gcagcaccag atagaggagg ccggcggccc agaggaccac caagacggcg
     4141 agcgacaggg cgaagaggaa ggcgccatag gcgaggctgg ggccgggtgc cgcacgcagg
     4201 tccgcccagc cggcctggag ccagtccagc gccgccgatg ccggcaggtg gcgggcgaag
     4261 gcgctcggcg gccgaagtgg cgggtggagg gtcggcagcg gctccaggat cgcgcgtctt
     4321 ttcatcgaac ggcctttgcg caaggggtca gcatgcgggc ttcgcggccg gcctgtcgag
     4381 cgtgggcgcc acgcaggcgg gctgcgaggt gaaggccacg gtcacgagat gcacgttggc
     4441 caggacgaag acgccggcga gcaccagggc ggccaggatg gcgaggcgct tctggctcca
     4501 gcgcccgctc atcggccgac ccctcgtcgg ctggaccccc gtcgattgcc ccctcatcgg
     4561 ctcgccagag cctcgtgctc ggcggcgagc cccgacacgt agaccgccag aaggttgatc
     4621 tcggcctcgc tcaaccgatc cgcccaggtc ggcatgacac cctggcggcc gcgctgcagg
     4681 gtggcgaaga tcgtggcgcg atcgccgccg tagatccaat gaccgtcgac gagcgagggg
     4741 gcgccgatcc cgagcccacc cacgccgcca tcgccgtggc agctcgcgca gttgtcggcg
     4801 aacagcgtcc cgccctcgcc gtcgggatcg gcctggccgg tcgagagcgc ctgcacgtag
     4861 ctggtcagcg tctcgatctc ggcccgctcc agcatctggt cgtggccgaa ggccggcatc
     4921 tgcgcgaagc gcgtctcggg gtgcgggctg ttgatgccga cgcgcagcgt ctcggcaatg
     4981 gtcgcaggat cgccccccca gagccagtcg ctgtcggcga ggtcgggaaa gccgggcccg
     5041 ccggtcgcat ccgtgccgtg gcaggcggcg cagttgtcga ggaagagccg gtgcgccgcc
     5101 ggcatcgcct tcgccatgag ggcctggtcg gccacgatgg catcgaagtc gcctgcgacg
     5161 aagcgggcca tccagtcctc gcggccgtcc gccagccgct ggtaccccgc cacggccatc
     5221 tcgccctggt cgagcccgag caggccgcgc gtgtagtccc ggccggtggg ccaggccggc
     5281 agcagcagcc aggcgatgat cgaatagacg acggtcaggc cgagaatcgt gatcacgaag
     5341 atcggaaacg gcgtgttgag ctccttgatg ccgttccagt cgtggccggt ggtctcgtag
     5401 ccggtcaccg gatcgacgtc cggggcctcg ccgtcatgcg cgctcatttc cgctccccgg
     5461 gcccgtcgtc gaggggccgg tcggcgcctg gcaggatgga gagcgccacg cgctcgtagg
     5521 cggccttcct gctcggccgg aaggccagca ccgcgacgat cacgaagaag cccatcatcc
     5581 agaagaggcc gaaggtcttg gcgaggacca ccagcgtctc gtgcgacggc atcatggcgc
     5641 ctcctccggc actttcacct cctccgtctc gaaggccaca tcggtcagcg tgccgagcga
     5701 ctggagatag gccaccagcg cgtccatctc cgtcaggcgg gcgggatcgc cgtcgaaagc
     5761 gcgaatggcg accttctcgc cgtagcgcgt ggcgacatcc gccgccccgt cgctgtccgg
     5821 attgccctgg gccagcgcgt cggccgcggc gttcgtcgcc atctcctcgg tgtaggggac
     5881 cccgaggcgg gcgagcgtgg cgagctcggc gccgagattg ccggtgtcga gcggctccat
     5941 cagccaggga taggccggca tgatcgaggc gggcacgacg gcgcgcgggt cgatcaggtg
     6001 ggccacgtgc caggggtcgg aatacttgcc gccgacccgc gccagatccg gcccggtgcg
     6061 cttcgacccc cagagcatgg gatggtcgta ggccgattcc gacggcaggg aataggggcc
     6121 gtagcggtcg atctcgtcgg ccagggtgcg gatcatctgg ctgtggcagg catagcagcc
     6181 ctcgcgcatg aagatctcgc ggccggcgag ctcgagcggc gtgtgcgggc gcatgtcgtc
     6241 cgggatcgtc accgtctcgt cgacggtgaa gagcggggcg atctcgacga tgccgccgat
     6301 cgaggcggcg acgatgatcg ccgccaccag ccccatggag atcttctcga ggttgtagtg
     6361 cagcctcagc atcggctcac tccgccggct cgggcagggc cggcccgggc aggagcgggc
     6421 ggtcgtgcgc ctcggcaccc gccctggcgg accgcatcgt ggcccaggtg ttcagggcgc
     6481 agatgacggc gccggcgagg aagagggcgc cgccgatggt gcgcaggagg tagtagggcg
     6541 ccatggccat gaccgattgc aggaagctgt aggagagcgc tccgctcgcg tcataggtcc
     6601 gccacatcag cccctgggtg atgccggcgt tccagagcgc cacgacgtag accagggtgc
     6661 cggtgacggc gagccagaag tgccattcga tggcgcgggg ccaggccatg gtctcgcgcc
     6721 ccgagagctg cggggcgagg gtgtagatgg cgccgaagat gatcatcgcc acccagccga
     6781 gcgtgccgga atggacgtgg ccgacggtcc agtcggtgta gtgcgagagc gagttgaccg
     6841 gccggatcgc gaggaacgag ccctcgaagg tggagagccc gtagaacacg gcggcgacga
     6901 acatgaagcg cagcgtggcg tcgtcgcgca ccttgtgcca ggcgccgttg agcgtggcga
     6961 tggcgttgcc cgcggcggcc cagctcggca ccaggagcat gacggagaag gtcatgccga
     7021 gcgtctgcac ccagtagggc aaggcggtgt agtggagatg gtgcgagccg acccagacgt
     7081 agaagaagac gatgccccag aagctgagga tcgagagccg gtaggaccag atcggccgcc
     7141 ccgagcggac cggcaggaag tagtagagca tggcgaggaa gccggcggtc aggaagaagg
     7201 ccaccgcgtt gtggccgtac caccactgca ccatggcatc ctgcacgccg gcccagatgg
     7261 tgaagctctt ggccccggcg aggctcaccg gcagggccag gttgttgacg atgtgcagca
     7321 tcgccacgac caggatgaag gcgaggtagt accagttcgc gacatagatg tgcggctcgt
     7381 tgcgccgtgc cagcgtgcgc atgtagagga cgaagtagac gacccagacg acgaccagcc
     7441 agaggtcggc gtaccactcg gcctcggcgt actccttgga ctgggtgctg cccatgaggt
     7501 agcccgagac cgcccagagg cagaagaggt tgaagccgat caggacgacc cagggggtga
     7561 ccgaatcggc gagccgcgcg cgcgaggtcc gctgcacgac gtggaacgag gtggcgatca
     7621 gcgcgttgcc gccgaagccg aagatgatgc cggaggtgtg gaccgggcgc aggcggccga
     7681 agctggtcca gggccagagc ggcgtcgcct cgggccagta gagcagggcc gcgacccaga
     7741 cgccgaccgc catggcgaag acggcccaga tcatcgtcat gacgatgccg aagcgggtcg
     7801 gctcgtcgta gtagcgggcg agccggtcgc cccggggctc cggctcggcg agtccgccgc
     7861 ccagggcgaa gagcagggcc acgccgacgg cgatcgccat ggcgccgtgc accgccatcg
     7921 gcccgacctt cgcggcgccg agcatgacga ggccgagcag ggcgagcgcc acggacaagg
     7981 cgatgccgag ctggcgttcg ctggagctga gggagcggat catggcaacg tccttctgcc
     8041 gcgtctcgag ccgtggcggg tcgcgtcgtg gtggccggcg aggccatgac gcccgggcgc
     8101 tcgcccgatg cgcgtcggca cccaggccgg aaccggcgag cgggtcgaga cgcatgatcg
     8161 ccggctgtcg gcttaatcgg tattcagaat gtctattagc acgaaggcac cggatcgcaa
     8221 ctccgaattc tcggcgaacg tctgtcacgc cggtgccgtg cggcgaccgg cgttcggatc
     8281 gaaggacgcg gagcgcgctc tctctccaga gcgctccggc gggcgacgca gatcggcccg
     8341 acaggcgggc caaacgcctt aggatttata acctatttcg aaccgactgt ccagaggtcg
     8401 tcgggttcga tcggcaaatc aggcttgccg gccggcgggc gggcccggaa aggcggcgaa
     8461 ggccgcgaca gggttggtcc ggggctcccg ggttcgttgg tcgctcgtcg ggttcgttcg
     8521 cgattcggtg ggttcgatcg tcgaatcggc aggttcgttg gtcgaatccc tgggttcgtt
     8581 cgcaggcccg gcgggttcgt tcgtccggaa ctgtcgcgga atccgcaaca tttgtcataa
     8641 tcgtgtcgcg gaatacgcga caacccgagg ggggatgttg cagaatccgc gacatttgtc
     8701 ataaccatgt cgcagattcc gcgacattct tgggttcggc gaccacgaat gtcgcatccg
     8761 gcgcgccatc ccgagggtcg gcgagcgggt gggcggaggg cctcggcggg atttttcggg
     8821 gaaatgcggc ggcgttcggc aattgtcgtc gtccgccccg tgaaattcgt cgtcatttat
     8881 cgccaggtgc tcgccttttc gtcggtcgaa
//
LOCUS       DAOTFI010000206         8859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9444_length_8859_cov_7.236824, whole genome shotgun sequence.
ACCESSION   DAOTFI010000206 DAOTFI010000000
VERSION     DAOTFI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9444_length_8859_cov_7.236824"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /gene="uvrB"
                     /locus_tag="P5558_18205"
     CDS             complement(<1..1733)
                     /gene="uvrB"
                     /locus_tag="P5558_18205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRY26310.1"
                     /translation="MQPAESASPLDLLKSLADPELLVSRPEAGRPFELVSEFQPSGDQ
                     PTAITELIGGIAEGQRDQVLLGVTGSGKTFTMAQVIADRQRPAIILAPNKILAAQLYG
                     EFKSFFPNNAVEYFVSYYDYYQPEAYVPRSDTYIEKTASVNEQIDRMRHSATRALFER
                     DDVIIVASVSCIYGIGSPETYARMTLNLREKQRVERQRLLRALVELQYKRNELDFSRG
                     AFRAKGDTIEIFPAHLDDQAWRISMFGDEIEAIWAFDPLTGQKGERLQQIRLYPNSHY
                     VTPKPTLAQAVESIKIELKATLEDFRTTGKLLEAERIEQRTVADIEMILTTGSCPGIE
                     NYSRYLTGRKPGEPPPTLFEYIPEHAILFVDESHVAVPQVGGMYKGDRQRKSTLAEFG
                     FRLPSCVDNRPLKFEEWDAMRPQTVYVSATPGPWELDRTGGVFVEQVIRPTGLVDPPV
                     IVRPVEHQVDDLLAECRVCREQGLRVLVTTLTKRMAEDLSEYLHEQGVRVRYMHSDID
                     TLERIEIIRDLRLGKFDVLVGINLLREGLDIPECGLVAILDADKEGYLRSETSLVQTI
                     GRAARNIDGRC"
     gene            1870..2484
                     /locus_tag="P5558_18210"
     CDS             1870..2484
                     /locus_tag="P5558_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRY26311.1"
                     /translation="MRLYHSQTSPYVRKVMVVAREAGIHDRIELIAGSASPVGETAPV
                     ARDNPLGKLPCLLLQDGGVLYDSRVICEYLDSQHDGHKLFPAAGPARWEALRRQALGD
                     GICDAALLRRYEASQRPLERQWDEWVLGQKRKIDRALDALEAEVPSFPASPDIGLIAV
                     AIALAYLDLRFAEDAWRDGRPGLTAWQAGFAMRPSMRETAPPAQ"
     gene            complement(2500..3744)
                     /locus_tag="P5558_18215"
     CDS             complement(2500..3744)
                     /locus_tag="P5558_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY26312.1"
                     /translation="MTLRGRIFLATPGPTNVPDRVLRAMHRAAEDFAAPAFSGVARSC
                     LDDLAKVFRTTGEVFSFIGNGHAAWEVPLVNLVEPGDTVLMPVSGRFSHYWAEMAEKL
                     GLRTERLEMDRRLPLDVGRIADRLRADTEHRIKALLVVQTETATGIRSDIEAIRRALD
                     EVRHPALFVVDAVASLAVEPFAMDDWGIDCALTASQKGLMSTPGLCFTAFNERALEVA
                     ESCRHPRRYFDICFRRGAMSYQWFHGTPPLQQIWGLREALDMLFEEGLGEVVERHRRL
                     ADAVRACVARWSEAGALSFHAMDPASRANGVTAIHTAPGIDPDAMRRSARDTYSTALG
                     AGLGELEGRAFRIGHLGDLNEPMILGTLASVELAMRRSAIPFGRGGIEAAIESLALAD
                     AGVEAMSPARTTEPASLGITAA"
     gene            3920..4654
                     /locus_tag="P5558_18220"
     CDS             3920..4654
                     /locus_tag="P5558_18220"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRY26313.1"
                     /translation="MRRALLLVLGLLSCHAATAQAATPAARCMAIIAVLEAAGEGEAG
                     MAAVIEVVKNRTADPRFPDEACAVVAQDRQFQPVGEWPALKRALAQPAALDPHAMLAG
                     GPVRRSLDRAFRLVSAGPSSLTGGALYFVNPRAMDPRHCDWFAGLKRTAKIGNHVFMT
                     HYRKGERRAAPALDCSDPMIGSLLNAKSSLARQYAQGLFHPDGPRIASRTPTPAELKA
                     WRRTGRLDARAAELKKHFKPGWISLE"
     gene            complement(4679..5080)
                     /locus_tag="P5558_18225"
     CDS             complement(4679..5080)
                     /locus_tag="P5558_18225"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY26314.1"
                     /translation="MPLARLDHVNVRTSQLQAMIDWYGKILDMQPGPRPDFPFGGAWL
                     YCDGFPIVHLVEVEEEPAAIAPKLEHFAVSATGLEEFLKHLEAHDVPYEVGLPPGFPI
                     IQVNFHDIDRNHIHVDFPTDESAALAKRGLA"
     gene            5164..6336
                     /locus_tag="P5558_18230"
     CDS             5164..6336
                     /locus_tag="P5558_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY26315.1"
                     /translation="MTSSRLGVVVALGTTQTLAFGSTYYLPAILAAPMAETLGLGVGW
                     VYAAFSIALLVSGVLGPVVGSRIDRLGGRDVLAASSLIFALGLGLLAATSGPSLLVLA
                     WLLIGVGMAMGLYDAGFATLAGIYGREARAPITGITLIAGFASTVAWPLTTLLEQSVG
                     WRGACVFWALVHLLLCLPINRWLVPRGTQKLPEVIQGAPLRTRQPGELRAMLALGFVF
                     AVSWFVSTAYAAHLPGLLLTAGLTPAMALFAATLVGPAQVAARLVEFSLLRRYHPLVS
                     ARLATLAHPIGAAALLAFGAPAAVLFTVLHGAGNGILTIAKGTLPLALFGPVGYGLRQ
                     GLLTAPSRFAQAAAPFLMTLAIEHLGLGVLAVSGGLLLLACLALFAVPRSLGPPTG"
     gene            complement(6347..7216)
                     /gene="ccoP"
                     /locus_tag="P5558_18235"
     CDS             complement(6347..7216)
                     /gene="ccoP"
                     /locus_tag="P5558_18235"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRY26316.1"
                     /translation="MATVERDEVTGVDTTGHEWDGIKELNNPLPKWWLYVFYASIAFS
                     VLWWILFPAWPSSTGYTPGLLGSNQRLELDTRLAEARAAQAVFLERIAAADAAEVAAD
                     PELLQFALAGGERHFKDNCAGCHGLGGAGQLHYPTLADDAWIWGGSLDSIQQTIRHGI
                     RNEDDPDARFGIMPAFGADEILSREEIAGVVQYVLALGGQDHDAALAATGQETYELNC
                     AACHGEDGKGIRDLGAPNLTDAVWLYGNTPEEIAAQISRPQHGVMPAWQGRLPEEVIT
                     MLTVYVHNLGGGE"
     gene            complement(7203..7394)
                     /locus_tag="P5558_18240"
     CDS             complement(7203..7394)
                     /locus_tag="P5558_18240"
                     /inference="COORDINATES: protein motif:HMM:NF017366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRY26317.1"
                     /translation="MVDTLAEWANAVWGLWLMLIFIGIVAWVFWPGRKKEMEENARIP
                     FEDQPDDLPGEKEKTNGDR"
     gene            complement(7416..8147)
                     /gene="ccoO"
                     /locus_tag="P5558_18245"
     CDS             complement(7416..8147)
                     /gene="ccoO"
                     /locus_tag="P5558_18245"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRY26318.1"
                     /translation="MNLIKHHGKIETRASVLAILILITVSIGGLVEIVPLFKIESTIE
                     KVEGVRPYSPLEQVGRNIYIREGCYNCHSQMIRPFRDEAERYGHYSLAAESMYDHPFQ
                     WGSKRTGPDLARVGGKYSNDWHVAHMVDPRAVVPESIMPGYHFLLEREVGYHDIAEHL
                     RTLGIVGVPYTEEMIESAAADLRDQATPDADYDGLLARYPKAVVGDLDGRPNEVTEMD
                     AVIAYLQILGRMVDFTDIRTEDILQ"
     gene            complement(8159..>8859)
                     /locus_tag="P5558_18250"
     CDS             complement(8159..>8859)
                     /locus_tag="P5558_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRY26319.1"
                     /translation="YIWAGPHHLHYTALPDWAQVLGMTFSIMLWMPSWGGMINGLMTL
                     SGAWDKLRTDPVLRFLVVSVAFYGMSTFEGPLMSIRQVNALSHYTDWTIGHVHSGALG
                     WVAFVSFGAVYYLVPRLWQAQRLYSLRLVEWHFWIATLGIVLYITAMWVSGIMQGLMW
                     RSYDSLGFLQYSFVETVAAMHPYYVIRAFGGVLFLSGALIMVYNLIRTMRREIRVEEE
                     PGRVMAGPVPAPAE"
BASE COUNT         1369 a         2973 c         3030 g         1487 t
ORIGIN      
        1 atgcagcgcc cgtcgatgtt gcgggcggcg cggccgatgg tctgcaccag cgaggtctcc
       61 gagcgcagat agccctcctt gtcggcatcg aggatggcca ccagcccgca ttcggggatg
      121 tcgaggccct cgcgcagcag gttgatgccg accagcacgt cgaacttgcc gaggcggaga
      181 tcgcggatga tctcgatgcg ctcgagggtg tcgatgtcgg aatgcatgta gcggacccgc
      241 acaccctgct cgtgcaggta ctcggagagg tcctcggcca tccgcttggt cagcgtggtg
      301 accagcacgc gcaggccctg ctcgcgacag acccggcatt cggccagcag atcgtcgacc
      361 tgatgctcga ccggccgcac gatgaccggc gggtcgacga ggccggtcgg ccggatcacc
      421 tgctcgacga agacgccgcc ggtgcggtcc agctcccagg ggcccggcgt cgccgagacg
      481 tagaccgtct gcggccgcat cgcgtcccac tcctcgaact tgagcggccg gttgtcgacg
      541 caggagggca ggcggaagcc gaactcggcc aaggtcgact tgcgctgccg gtcgcccttg
      601 tacatgccgc cgacctgggg cacggcgaca tggctctcgt cgacgaacag gatggcgtgc
      661 tcgggaatgt actcgaacag cgtcggcggc ggctcgcccg gcttgcggcc ggtgaggtag
      721 cgggaatagt tctcgatgcc gggacaggag ccggtggtca ggatcatctc gatatcggcc
      781 acggtgcgct gctcgatgcg ctcggcctcg agcagcttgc cggtggtgcg gaagtcctcg
      841 agcgtcgcct tgagctcgat cttgatcgat tcgaccgcct gggcgagcgt cggcttgggc
      901 gtgacgtaat ggctgttggg gtagaggcgg atctgctgca gccgctcgcc cttctgtccg
      961 gtcaggggat cgaacgccca gatcgcctcg atctcgtcgc cgaacatcga gatgcgccat
     1021 gcctggtcgt cgagatgggc cgggaagatc tcgatcgtgt cgcctttcgc ccgaaaggcg
     1081 ccgcggctga agtccagctc gttgcgcttg tactgcagct cgaccagggc acgcagcagg
     1141 cgctgccgct cgacccgctg cttctcgcgc aggttcaggg tcatcctggc atacgtctcg
     1201 ggcgagccga tgccgtagat gcaggagacg gacgccacga tgatgacgtc gtcgcgctcg
     1261 aagagggccc gggtggcgct gtggcgcatc cggtcgatct gctcgttcac gctcgccgtc
     1321 ttttcgatat aggtgtccga gcggggcaca taggcctcgg gttgatagta gtcgtagtag
     1381 ctgacgaaat attcgaccgc gttgttcggg aagaaggact tgaactcgcc gtagagctgt
     1441 gccgccagga tcttgttggg cgcgaggatg atcgccgggc gctgccggtc ggcgatgacc
     1501 tgcgccatgg tgaaggtctt gccggagccg gtgacgccga gcagcacctg gtcgcgctgc
     1561 ccttccgcga ttccgccgat cagttcggtg atcgccgtcg gctggtcgcc cgacggctgg
     1621 aactcggaaa ccagctcgaa cggcctgccg gcctcgggcc gggagaccag cagctccggg
     1681 tcggcaaggc tcttgagcag gtcgagcggc gacgcgcttt cggctggctg catggatggc
     1741 ccgttcggat cgacaggtgg gagctaggga aagaatgagg atggagccta gccgggccga
     1801 cctcaagggc gctatgggaa aaacgccgcc ggcggtgatc ccgatcggcc tgtcctggag
     1861 ggaacccgca tgcgcctgta ccacagccag acctcgccct atgtccgcaa ggtcatggtg
     1921 gtagcccggg aggccggtat ccatgaccga atcgagctga tcgcgggaag cgcgtcgccg
     1981 gtaggcgaga ccgctcccgt cgccagggac aatccgctcg gcaagctccc ctgtctgctg
     2041 ctccaggatg gcggcgtgct ctacgacagc cgggttatct gcgagtacct cgacagccag
     2101 cacgacggac acaagctctt ccccgctgcc ggcccggccc gttgggaggc gctgcgtcgc
     2161 caggcgctcg gcgacggcat ctgcgatgcc gccctgctgc gccgctacga ggcttctcag
     2221 cggccgctcg aacgacaatg ggacgaatgg gtgctcggcc agaaacgcaa gatcgaccgc
     2281 gccctcgatg cgctcgaggc ggaggtcccg agctttccag catcgcccga catcggcctg
     2341 atcgccgtcg ccatcgccct cgcctatctc gacctgcggt tcgcggaaga cgcgtggcgc
     2401 gacggccggc cgggcctcac ggcgtggcag gccggttttg cgatgcggcc gtcgatgcgg
     2461 gagacggcgc cgcccgccca gtaggcagcc agggttggct caggctgccg tgatgccgag
     2521 gctggcgggc tccgtcgtgc gtgccgggct catcgcctcg acgccggcat ccgccagcgc
     2581 caggctctcg atcgccgcct cgatgccacc ccggccgaac gggatggccg agcgacgcat
     2641 cgccagctcc acgctggcga gcgtgccgag gatcatcggc tcgttgaggt cgcccagatg
     2701 gccgatgcgg aaggcccggc cctccagctc gccgagcccg gcgccgagtg ctgtcgaata
     2761 ggtgtcgcgg gcagagcggc gcatggcgtc cggatcgatg ccgggggcgg tatggatggc
     2821 ggtgacgccg ttggcgcggc tggccggatc catggcgtgg aagctcaggg cacccgcctc
     2881 gctccagcgg gcgacgcagg cgcgaaccgc atcggcaagc cggcgatgtc gctcgacgac
     2941 ctcgcccagc ccctcctcga acagcatgtc gagcgcctcg cgcaggcccc atatctgctg
     3001 caggggcggc gtgccgtgaa accattgata gctcatggcg ccgcggcgaa aacagatgtc
     3061 gaaatagcgc ctggggtggc ggcaggactc ggccacctcg agcgcacgct cgttgaacgc
     3121 cgtgaagcag aggcccgggg tcgacatcag gcccttctgc gaagcggtga gcgcgcagtc
     3181 gatgccccag tcgtccatgg cgaacggctc gaccgcgagg ctggcgacgg cgtcgaccac
     3241 gaagagcgcc ggatgacgca cctcgtcgag cgcgcgccgg atggcctcga tgtcgctgcg
     3301 gatgccggtc gcggtttcgg tctggacgac cagcagggcc ttgatccggt gctcggtgtc
     3361 ggcgcgcagg cgatcggcga tccggccgac atccagcggc agccggcgat ccatctccag
     3421 ccgctcggtc ctgagcccga gcttctccgc catctcggcc cagtagtgcg agaaccggcc
     3481 ggagaccggc atcagcaccg tgtcacccgg ctccacgagg ttgaccagcg gcacttccca
     3541 ggcggcatgg ccgttgccga tgaagctgaa gacctcgccc gtcgtgcgga agaccttcgc
     3601 cagatcgtcg aggcaggacc gggcgacgcc gctgaaggcc ggggcggcga agtcctccgc
     3661 ggcgcgatgc atcgcacgca gcacccggtc gggcacgttg gtgggccccg gtgtcgccag
     3721 gaaaattcgt ccgcgcagcg tcatgcgttc ctcgagtggc ggtgcatggg caggaactag
     3781 cgaagtggcg ggccttcgga aacccgtaaa gcctaacacg aggtcgcact cgaaggtcga
     3841 ggcggcagcc cggcaattga cgcgacatgg tgaacgaacg tttaatcacg acaccggaaa
     3901 agccagcgag gagatcgagt tgcggcgtgc gttgctcctg gtgctgggct tgctttcctg
     3961 ccatgcggcc actgcccagg cggcgacgcc ggcggcgcgc tgcatggcga taatcgcggt
     4021 gctggaggcc gccggcgaag gcgaggcggg gatggcggcc gtgatcgagg tggtgaagaa
     4081 ccgcacggcg gacccccgct ttcccgacga ggcctgtgcc gtggtcgccc aggatcgcca
     4141 gttccagccg gtcggcgaat ggcccgctct gaagcgcgcc ctggcccagc cggcagcgct
     4201 cgatccgcat gccatgctcg ccggcggccc ggtgcgccgc agcctcgacc gggcgttccg
     4261 cctggtctcc gcgggtcctt cgtcgcttac cggcggtgcg ctctacttcg tcaacccccg
     4321 tgccatggac ccgcgccact gcgactggtt cgccggcctg aagcgcaccg ccaagatcgg
     4381 caaccatgtc ttcatgaccc actaccgcaa gggcgagcgc cgggcggcgc cggctctcga
     4441 ctgcagcgat ccgatgatcg gcagcctgct caacgcgaag tccagcctcg cccgccaata
     4501 cgcgcaaggc ctcttccacc cggatggtcc gaggattgcc agccgcacgc cgaccccggc
     4561 ggagctcaag gcctggcgcc gcaccggccg tctcgatgcc cgtgccgccg agctcaagaa
     4621 gcacttcaag cctggctgga tctcgctcga gtgacgcgag actgtcggcg gatggtcctc
     4681 aggccagccc gcgtttggcc agcgccgccg actcgtccgt cgggaagtcg acgtggatgt
     4741 ggtttcggtc gatgtcgtga aagttgacct ggatgatcgg gaagcccggc ggcaggccaa
     4801 cttcgtaagg gacgtcgtgc gcttccagat gcttgagaaa ttcctcgaga ccggtggcgc
     4861 tgacggcgaa atgctcgagc ttgggggcga tggcggccgg ctcttcctcg acctcgacga
     4921 gatgaacgat ggggaagcca tcgcagtaca gccaggcgcc gccgaacggg aagtcgggcc
     4981 gggggccggg ctgcatgtcg aggatcttcc cgtaccagtc gatcatcgcc tgaagctggc
     5041 tggtgcgcac attgacatgg tcgagccgtg cgagcggcat ggctgttcct ccctgatggc
     5101 atctgcccca tatctagcgc gatcgatcac cgagcgacag cgcccccgca gcggccggcc
     5161 ccgatgacga gttccaggct cggcgtggtc gtcgccctcg gcacgacgca gaccctggcg
     5221 ttcggctcca cctattacct gccggcaatc ctcgctgccc ccatggcgga aacgctgggc
     5281 ctgggagtcg gctgggtcta tgcggcgttc tcgattgccc tgctcgtttc cggcgttctc
     5341 gggcccgtgg tcggcagtcg catcgaccgg ctgggcgggc gcgatgtcct tgccgcttcc
     5401 agcctgatct tcgccctggg cctcggcctc ctcgcggcga cctcgggccc ttcgctcctg
     5461 gtcctcgcct ggctcctgat cggcgtcggc atggccatgg gcctctacga cgccggattc
     5521 gccacgctcg ccggcatcta tgggcgcgag gcgcgggcgc ccatcaccgg catcaccctg
     5581 atcgccggct tcgccagcac cgtcgcctgg ccgctcacca ccctgctcga gcaatcggtc
     5641 ggctggcgtg gcgcctgcgt cttctgggca ctcgtccatc tcctgctctg cctgccgatc
     5701 aatcgctggc tggtgccgcg cggcacccag aagctgccgg aggtgatcca gggcgcgccg
     5761 ctgcgcaccc gacagccggg cgagctgcgc gcgatgctcg ccctgggctt cgtcttcgcc
     5821 gtgagctggt tcgtcagcac ggcctatgcc gcgcacctgc cgggtctgct gctcactgcc
     5881 ggcctgaccc cggccatggc cctcttcgcc gcgacgctcg tcggacccgc ccaggtcgcg
     5941 gcgcggctgg tcgagttcag cctgctgcgc cgctatcacc ccctggtctc ggcgcggctc
     6001 gccaccctgg cgcacccgat cggagcggca gcgctgctcg ccttcggtgc cccggccgcg
     6061 gtcctgttca ccgtcctgca tggtgccggc aacggcattc tcacgattgc caaggggacg
     6121 ctgcccttgg cgctcttcgg gccggttggg tacgggctgc ggcagggcct gctaacggcc
     6181 ccctcgcgct ttgcccaagc cgcggcacca ttcctcatga cgctggccat cgagcacctg
     6241 ggcctcggcg ttctcgctgt ctccggcggg ctcctgctgc tggcctgcct cgccctcttc
     6301 gccgtgccgc gctcgctcgg gccgccgacc ggttgatcct gctcgcctac tcgccgccac
     6361 cgagattgtg gacgtagacg gtgagcatgg tgatgacttc ctcgggcagc cgaccctgcc
     6421 aggccggcat gacaccgtgc tgcggcctcg agatctgggc ggcgatttcc tcgggcgtgt
     6481 tgccgtagag ccagacggca tcggtcaggt tgggggcgcc cagatcgcgg atgcccttgc
     6541 cgtcctcgcc gtggcaggcg gcacaattca gctcgtacgt ctcctgcccg gttgccgcca
     6601 gcgcagcgtc atggtcctgt ccgccgagcg ccagcacgta ttgcacgacc ccggcgatct
     6661 cctcgcggct gaggatctcg tcggcgccga aagccggcat tatgccgaag cgcgcgtcgg
     6721 gatcgtcctc gttgcgaatg ccgtgcctga tggtctgctg gatcgagtcg agcgagccgc
     6781 cccagatcca cgcatcgtcg gccagagtcg ggtagtgcaa ctggccggcg ccgcccaacc
     6841 cgtgacaacc cgcacagttg tccttgaagt gacgctcgcc acccgccagg gcgaactgca
     6901 ggagctcggg gtccgcggcg acctccgccg catccgccgc ggcgattcgc tcgaggaaca
     6961 ctgcctgggc tgcccgggcc tcggccagcc gcgtatccag ctccaagcgc tggttcgagc
     7021 cgagcagccc cggggtgtag ccggtcgagc tgggccaggc cgggaagagg atccaccaga
     7081 gcaccgagaa ggcgatgctg gcatagaaga cgtagagcca ccatttgggc agcgggttgt
     7141 tgagctcctt gatcccgtcc cattcgtggc cggtcgtatc gaccccggtg acctcgtccc
     7201 tctcaacggt cgccattcgt cttctccttc tcaccgggca ggtcgtcggg ctggtcctcg
     7261 aacggaatgc gggcgttctc ttccatctct ttctttcggc cgggccagaa cacccaggcg
     7321 acgatgccga tgaagatcag catcagccac aggccccaaa cggcgttcgc ccattcggcg
     7381 agcgtgtcga ccacgagcga tacgcccttg cgccgctact gcaggatgtc ctcggtgcgg
     7441 atgtcggtga agtccaccat ccggccgagg atctgcaggt aggcgatgac cgcgtccatt
     7501 tcggtgactt cgttcggccg gccgtcgagg tcgccgacga cggccttggg atagcgcgcg
     7561 aggagaccgt catagtcggc atcgggcgtc gcctggtcac gcagatcggc ggcggcgctc
     7621 tcgatcatct cttcggtata gggcacgccg acgatgccga gcgtgcgcag atgctcggcg
     7681 atgtcgtggt agccgacctc gcgctccagg aggaagtgat agccgggcat gatgctctcc
     7741 ggcacgacgg cccggggatc gaccatatgc gcgacgtgcc agtcgttcga gtacttgccg
     7801 ccgacccgcg cgagatccgg cccggttcgc ttgctgcccc attggaaggg gtggtcgtac
     7861 atgctctccg cggcgaggct gtagtggcca tagcgctcgg cctcgtcacg gaacggccgg
     7921 atcatctggc tgtggcagtt gtagcagcct tcgcggatgt agatgttgcg gccgacctgc
     7981 tcgagcggtg agtaggggcg gacaccctcg accttctcga tcgtcgattc gatcttgaac
     8041 agcgggacga tctcgacgag cccgccgatc gagacggtga tcaggatgag gatggcgaga
     8101 accgaggcgc gcgtctcgat cttgccatga tgcttgatca ggttcatcgg ggcgctcctt
     8161 attcggccgg ggcgggcacg ggtcccgcca tgacccgacc aggctcttcc tcgaccctga
     8221 tctcgcggcg catggtccgg atcagattgt agaccatgat gagcgcaccg ctgaggaaga
     8281 gtacgccgcc gaaggcccgg atcacgtaat agggatgcat ggccgccacc gtctcgacga
     8341 acgagtactg gaggaagccg aggctgtcat aggaacgcca catcaggccc tgcatgatcc
     8401 cagacaccca catcgccgtg atgtagagga cgatgccgag ggtcgcgatc cagaagtgcc
     8461 attcgacgag gcggagcgag tagagccgct gcgcctgcca gagcctgggc acgagatagt
     8521 agacggcgcc gaagctgacg aaggcgaccc agccgagcgc gccggaatgg acgtggccga
     8581 tggtccagtc ggtgtagtgc gagagcgcat tgacctgccg gatcgacatg agcggcccct
     8641 cgaacgtgct catgccgtag aaggcgaccg agaccacgag gaagcgcagg accgggtcgg
     8701 tgcgcagctt gtcccaggcg ccggagagcg tcatcaggcc gttgatcatg ccgccccagg
     8761 acggcatcca gagcatgatc gagaacgtca tgcccagcac ctgcgcccag tcgggcagcg
     8821 cggtgtagtg cagatggtgc gggccagccc agatataga
//
LOCUS       DAOTFI010000207         8770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9587_length_8770_cov_7.317958, whole genome shotgun sequence.
ACCESSION   DAOTFI010000207 DAOTFI010000000
VERSION     DAOTFI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9587_length_8770_cov_7.317958"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3463
                     /gene="putA"
                     /locus_tag="P5558_18255"
     CDS             <1..3463
                     /gene="putA"
                     /locus_tag="P5558_18255"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HRY26320.1"
                     /translation="MEAFLDEYGLSTGEGVGLMCLAEALLRVPDAETMDDLIEDKIAP
                     SNWSAHLGQSFSPLVNASTWALMLTGSILDSDPAKPAAGARRLIKRLGEPVVRTAVGR
                     AMKIMGQQFVLGQTIEEGIKKAEKLRARGYTYSYDMLGEAARTEADAQALYESYAHAI
                     GRIAKAAKADIRDNPGISVKLSALSPRYQTTHRGAVAGEVIPRCLTLSRMAVEAKVGF
                     NIDAEEQDRLDVSLDVIEAVLSDDGLRGWDGFGVVVQAYGKRAPQVLRHLYALAERLD
                     RRIMVRLVKGAYWDTEIKLAQELGASSYPVYTRKVNTDVSYMSCARMLLDMRDRIYPQ
                     FATHNAHTCAAVLELAGTDRQSFEFQRLHGMGESLHDIVKKEAGTRCRIYAPCGPHKD
                     LLAYLVRRLLENGANSSFVNQIVNGDIAPETIAADPVDHALANDPIQNPIIRMPRDLF
                     KPRRENSIGYRLEEPASVLPLLEARDHFARTVWTGRPVTADDAAPEGPARDLTSPADR
                     GSIVGHVHEATEDEISRALDAAQRGFGDWAQTPVAIRSATLRRIADLYEENFAELCAI
                     AAREAGKILLDGVAEVREAVDFLRYYAAEAERLEDEEPGKARGVFVCISPWNFPLAIF
                     TGQIAAALAAGNAVVAKPAEQTPLIATRAAELFREAGVPKAVFQLLPGDGPSVGALLT
                     SDPRIAGVCFTGSTRTAQMIHKALAANAGADAVLIAETGGLNAMIVDSTALPEQAVDD
                     IVASSFQSAGQRCSALRMLYVQEDTMDHMIEMLKGAMDTLVIGDPWSLETDVAPVIDA
                     EAQADIEGYVDAQQKKGRVLKRLDAPGAGTFVGAALVEVEGIEDLEREVFGPVLHVAS
                     FKAKDIDRTIEAINARGFGLTFGLHTRIDDRVQQIVDRIRVGNTYVNRNQIGAVVGSQ
                     PFGGEGLSGTGPKAGGPNYVARFRRAEPPAEHPAPEAEPLSAERVGQALAALDATAWA
                     ARRDRVQVLRRALDGQGAAVRRAVNAAAAFDSGPFELPGPTGESNRLSMPPKGLVLCL
                     GPETGTALAQAAQALAAGCPVLVAAPGAAAAVASLAKAGAPVACIEGTVPAEALAQVP
                     DLVIVAAAGDSTWTRSLRKALASRAGRIVQLETEVICPARFYAERHICIDTTAAGGNA
                     SLLAAVA"
     gene            3506..4978
                     /gene="putP"
                     /locus_tag="P5558_18260"
     CDS             3506..4978
                     /gene="putP"
                     /locus_tag="P5558_18260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HRY26321.1"
                     /translation="MQLGVWVSLTIYFVAMLGIGFYAWRKSTANSEEYMLGGRDLPPA
                     VAALSAGASDMSGWLLLGLPGALYVAGLVEAWIGIGLFIGALANWMIVAPRLREQTER
                     YDNALTIPEFLANRFPTQATTLRMVSAVIIVLFFAVYTASGLVAGGKLFSTAFEGGYQ
                     TGVWLTLGIVLVYTVVGGFLAVCLTDFVQGLIMMLALIIMPAVVLYAGDGGGFGQAGE
                     TLSTVEGFTLSWWTDSMTFIGWLSLMAWGLGYFGQPHIIVRFMAVRTVAEVPTARNIA
                     MGWMGISLLGAIGVGVLGRAYVERSGLVLEDPETIFILLAETLFPAMITGFLFAALLA
                     AIMSTVSSQLLVSSSSLAEDIYKLLVDKQASDSTIVTVGRIAVVAVGAVAALIAGDPD
                     SEVLGLVSNAWAGFGAAFGPLMILSLTWQRMTGLGAVAGLVTGALTVITWIALGWNAS
                     FLGGPGIYEMIPGFILAMAAIVVVSLAGEAKGEFRPIAAA"
     gene            complement(5058..5131)
                     /locus_tag="P5558_18265"
     tRNA            complement(5058..5131)
                     /locus_tag="P5558_18265"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5097..5099),aa:Gln,seq:ctg)
     gene            5258..5334
                     /locus_tag="P5558_18270"
     tRNA            5258..5334
                     /locus_tag="P5558_18270"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5292..5294,aa:Asp,seq:gtc)
     gene            5548..5913
                     /locus_tag="P5558_18275"
     CDS             5548..5913
                     /locus_tag="P5558_18275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRY26322.1"
                     /translation="MDAYLLEYLPILIFLAIAIGLAAVMVLASMVVARQKPNTEKLSP
                     YECGFAAFEDSRGRFEVRFYLVAILFIIFDLEVAFLFPWAVSLGEIGLHGFWSMMLFL
                     AVLTVGFIYEWKKGALEWE"
     gene            5904..6452
                     /locus_tag="P5558_18280"
     CDS             5904..6452
                     /locus_tag="P5558_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRY26323.1"
                     /translation="MGVSRPLVRPAEAPAQPGPISLYEDLGRDLQDKGFFVTGFNDLV
                     NWARSGSLWPMTFGLACCAVEMMHAAASRYDLDRFGVVFRPSPRQSDVMIVAGTLCNK
                     MAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTA
                     EALVYGILQLQKKIRRTGTIER"
     gene            6449..7084
                     /locus_tag="P5558_18285"
     CDS             6449..7084
                     /locus_tag="P5558_18285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRY26324.1"
                     /translation="MTAEQQADLNESLQDLKKLVEDHLGDDLILAQLAHGELSITVAT
                     TDLPRALLWLRDDPNMLFKELVDICGVDYPEREQRFDVVYHLLSMHHNQRIRVRVTTD
                     EATPVPSTTGLFRSAGWFEREAWDMYGILFSDHPDLRRLLTDYGFEGHPLRKDFPLTG
                     YVEMRYDDEQQRVVYEPVKLRQDFRNFDFLSPWEGAGGLLPGDEKAVDPAA"
     gene            7122..8300
                     /locus_tag="P5558_18290"
     CDS             7122..8300
                     /locus_tag="P5558_18290"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRY26325.1"
                     /translation="MAEVDLRSFTVNFGPQHPAAHGVLRLVLEMSGEIVERADPHIGL
                     LHRGTEKLIEYRPYLQNIPYFDRLDYCSMLCQEHAYVLAVEKLLEVEVPERALYIRTL
                     FDEITRILNHLLNITTMALDCGAMTPLLWMFEEREKLMAFYERVSGARMHANYYRFGG
                     VARDMPAGLAEDIATWLDSFEPLFEDVDNLLSENRIFKQRTVDIGVVTAEDAFNLGFS
                     GPMLRASGVAWDLRKAEPYAMYDRMEFDIPVGRNGDCWDRYLVRMLEVKESMKIVRQC
                     LDQMRPGPVRTDDKKVTPPSRANMKRSMEALIHHFKLYTEGVHVPEGEVYAAVESGKG
                     EFGVYLVSDGTNRPYRCKIRSPGYVHLQALDEMSKGHQLADVVAIIGSMDIVFGEIDR
                     "
     gene            8297..>8770
                     /gene="nuoE"
                     /locus_tag="P5558_18295"
     CDS             8297..>8770
                     /gene="nuoE"
                     /locus_tag="P5558_18295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRY26326.1"
                     /translation="MSFSPDIATFSFAPELGAEIERIMARYPEARKQSAVMPLLHLAQ
                     KQHGGWLPRAALDHVADFLEIPRIRVYEVASFYDMYNTEPTGRIQVRVCTTTPCWLCG
                     SDDVVRAAKEVLGVGIGESTEDGRFFLREFECLGACCNAPMMWIDDDYYEDLTYER"
BASE COUNT         1401 a         2977 c         2902 g         1490 t
ORIGIN      
        1 gatggaggca ttcctcgacg agtacggtct ttcgaccggc gagggcgtcg gcttgatgtg
       61 tctcgccgag gcactgcttc gcgtccctga cgccgagacg atggacgatc tgatcgagga
      121 caagatcgcg ccgtccaact ggagcgcgca tctgggtcaa tcgttttcgc cgctcgtcaa
      181 cgcgtcgacc tgggccctga tgctgacggg gagcatcctc gactccgacc ctgcgaagcc
      241 cgcggccggc gcccgccgcc tgatcaagcg gctcggggaa cccgtcgttc gcaccgccgt
      301 cggacgggcg atgaagatca tgggccagca gttcgtcctt ggccaaacca ttgaggaggg
      361 catcaagaaa gcggagaagc ttcgtgcgag gggctacacc tactcctacg acatgttggg
      421 cgaggcggcg cggacggagg ccgatgcgca ggccctgtac gaaagctatg ctcacgccat
      481 cggccggatc gccaaggcgg ccaaggccga tatccgcgac aatccaggga tttccgtcaa
      541 gctctccgcc ctgtcgccca gataccagac cacccatcgc ggggctgtcg ccggggaggt
      601 gatcccacgc tgcctcacgc tctcgcggat ggccgtcgag gcgaaggtgg gcttcaacat
      661 cgatgccgag gagcaggacc ggctcgacgt ctcgctcgac gtgatcgagg cagtgctctc
      721 cgacgacggt ctcagaggct gggacgggtt cggcgtcgtc gtccaggcct atggcaaacg
      781 ggcgccccag gtcttgcgcc atctttacgc gctggccgag cggctggacc gccggatcat
      841 ggttcggctc gtgaagggcg cctattggga tacggagatc aagctggcgc aggagctcgg
      901 cgccagcagc tatccggtct acacccggaa ggtgaacacc gacgtctcgt atatgtcctg
      961 tgcgcgcatg ctgctcgaca tgcgcgatcg catctatccg cagttcgcga cccacaacgc
     1021 ccatacctgc gccgccgtcc tcgagctggc cggcaccgac cgccaaagct tcgagttcca
     1081 gcgcctgcac gggatgggcg aatcgctcca cgacatcgtc aagaaggagg ccgggacgcg
     1141 ctgccggatc tacgcaccct gcgggccgca caaggatctt ctcgcctatc tcgtcaggcg
     1201 gctcctcgaa aacggcgcca attcgagctt cgtcaatcag atcgtcaatg gcgacatcgc
     1261 ccccgagacc atcgccgccg atccggtgga ccacgcgctg gccaacgacc cgatccaaaa
     1321 ccccatcatc cggatgccgc gcgatctgtt caagcccagg cgcgagaact cgatcggcta
     1381 tcgcctcgag gaacctgcga gcgtcctgcc tctcctcgag gcgcgcgatc attttgccag
     1441 gacggtctgg accgggcgac cggtcaccgc cgacgatgcc gcccccgagg gacccgctcg
     1501 cgacctgacc tcgccggccg accgcggctc gatcgtcggg catgtccatg aggccaccga
     1561 ggacgagatc tcgcgcgccc tcgacgccgc gcagcgaggt ttcggggact gggcgcagac
     1621 gccggtcgca atcaggtcgg cgacgctgcg ccggatcgcg gatctctacg aggagaactt
     1681 cgccgagctt tgcgccatcg ccgcccgcga ggcggggaag atcctcctcg acggcgtggc
     1741 cgaggttcgc gaggccgtgg atttcctgcg ctactacgcg gccgaggcgg agcggctgga
     1801 ggacgaggag ccgggcaagg cgcgcggtgt cttcgtctgc atcagcccgt ggaacttccc
     1861 gctcgccatc ttcaccggcc agatcgcggc cgcactcgct gccgggaacg cggttgtggc
     1921 gaaaccggcc gagcagaccc cgctgatcgc gaccagggcg gccgagctct tccgcgaagc
     1981 gggcgtgccc aaggccgtct ttcagctcct gcccggcgac ggaccctccg tcggggcgct
     2041 gctgacctcg gacccgagga tcgcgggtgt ctgcttcacc ggatcgaccc gaaccgcgca
     2101 gatgatccac aaggcgcttg ccgcgaatgc gggcgccgac gcggtgctga tcgccgagac
     2161 cggcggcctg aacgccatga tcgtcgactc cacggcgctg ccggagcagg cggtggacga
     2221 tatcgtcgcc tcgtccttcc agtcggccgg ccagcgctgc tcggcgttgc ggatgctcta
     2281 cgttcaggaa gacacgatgg accatatgat cgagatgctg aagggtgcca tggacaccct
     2341 cgtcatcggc gatccctggt cgctcgagac cgacgtcgcg cccgtcatcg acgccgaggc
     2401 gcaggccgac atcgaagggt acgtcgacgc gcagcagaag aagggccgcg ttctcaagcg
     2461 tctcgacgcg ccgggcgcgg gcacgttcgt cggcgctgcg ctggtcgagg tcgaagggat
     2521 cgaggatctc gagcgggagg tgttcggccc ggtcctgcat gtcgcatcgt tcaaggcaaa
     2581 ggacatcgac aggacgatcg aggcgatcaa tgccaggggc ttcggcctga ccttcggcct
     2641 gcacacccgg atcgacgacc gggtccagca gatcgtcgac cgcatcaggg tcggcaacac
     2701 ctacgtcaac cgcaaccaga tcggcgccgt cgtcggcagt cagccgttcg gcggcgaagg
     2761 gttgtccggc accggcccca aggccggcgg accgaactac gtcgcccgct ttcgccgggc
     2821 cgagccgcct gcggagcatc cggcacccga ggccgagccc ctgtcggcag agcgggtcgg
     2881 ccaggcgctg gccgcactcg acgcgactgc ctgggcggcc cgccgcgacc gggtgcaggt
     2941 gctccgccga gccttggacg gccagggtgc cgcggtgcgg cgcgcggtca acgccgccgc
     3001 cgccttcgac agcggcccct tcgagctgcc cggacccacc ggcgaatcca accgcctgtc
     3061 gatgccgccc aagggcctcg tcctctgcct cgggcccgag accggcaccg cgctcgccca
     3121 ggccgcccag gcgcttgccg ccggctgccc ggttctggtc gcggcccccg gagccgcggc
     3181 cgccgtggct tcgctggcga aggccggcgc gccggtggcg tgcatcgaag gcaccgtgcc
     3241 tgccgaggcg ctggcgcagg tgccagacct ggtcatcgtc gccgccgcgg gcgactcgac
     3301 atggacccgg agcctgagaa aggcgcttgc cagccgcgcc ggccgcatcg tgcagctcga
     3361 gacggaggtg atctgcccgg cccgcttcta cgccgaacgc cacatctgca tcgacacgac
     3421 cgctgccgga ggcaacgcca gcctgctcgc cgccgtcgcc tgacaagaac cagagcaaga
     3481 cgagctcaag aaagggagag ccaaaatgca gctcggcgtt tgggtcagcc tgaccatcta
     3541 cttcgtggcg atgctgggaa tcgggttcta tgcctggcgc aagtcgaccg ccaactccga
     3601 ggagtacatg ctcggcgggc gggacctgcc accggcggtg gccgcgctct cggccggtgc
     3661 gtcggacatg tcgggctggc tgctgctcgg tctgccgggc gcgctctacg tcgccggcct
     3721 cgtcgaggcc tggatcggca tcggcctctt catcggggcg ctcgccaact ggatgatcgt
     3781 ggcaccgcgg ctgcgcgagc agaccgagcg ctacgacaac gcgctgacga taccggaatt
     3841 tctggccaac cgctttccga cccaggcgac aacgctccgc atggtgtcgg cggtcatcat
     3901 cgtgctgttc ttcgcggtct acaccgcctc cggcctggtt gcgggcggca agctcttctc
     3961 gacggccttc gaggggggtt accagacggg cgtctggctg acgctcggaa tcgtcctcgt
     4021 ctacacggtg gtcggtggct tcctcgccgt ctgcctgacg gatttcgtcc agggccttat
     4081 catgatgctg gcgctcatca tcatgccggc cgtggtgctc tacgcgggtg acggcggcgg
     4141 cttcggccag gcgggcgaga ccctctcgac cgtcgagggc ttcacgctct cgtggtggac
     4201 cgacagcatg accttcatcg gttggctctc gctgatggcc tggggcctcg gctatttcgg
     4261 ccagccgcac atcatcgtcc gcttcatggc ggtgcgcacc gtcgcggagg ttccgaccgc
     4321 acgcaacatc gcgatgggct ggatgggcat ctcgctgctc ggtgccatcg gcgtgggcgt
     4381 gctcggccgt gcctatgtcg agcgcagcgg gctggtcctc gaggatcccg agaccatctt
     4441 catcctgctc gccgagacgc tctttccggc tatgatcacc ggcttcctct ttgcggcgct
     4501 gctggctgcg atcatgtcga cggtgtcgag tcagctactg gtctcgtcct cgtcgctggc
     4561 cgaggacatc tacaagctcc tcgtcgacaa gcaggcgagc gattccacga tcgtgaccgt
     4621 cggccgcatc gccgtggtgg ctgtcggcgc cgtggcggcg ttgatcgccg gtgatcccga
     4681 cagcgaggtc ctcgggctcg tctcgaatgc gtgggccggg ttcggcgccg ctttcggccc
     4741 gttgatgatc ctgtccctga cgtggcagcg gatgaccggg ctcggcgccg tggcaggtct
     4801 cgtcacggga gccctgacgg tgatcacctg gatagcactc ggctggaacg cgagcttcct
     4861 cggcggaccg ggtatctatg agatgatccc gggcttcatc cttgcgatgg cggcgatcgt
     4921 cgtcgtcagc ctggccggcg aggccaaggg cgagttccgg ccgatcgctg cagcatgaca
     4981 acacgtcggc gcgcgatggc cggcgaggct cggatgaggg aacgggcgtg ccaggcttgc
     5041 caaggaattg cctagcctgg ctgggggacc tggattcgaa ccaggactgt tcggtccaga
     5101 gccgaaagtt ctaccgttaa actatccccc aaggcgtggc gggttcgtaa tgtccctgag
     5161 cctcgcatgt caagcgctct gccctgcggg gcttccgcgg gtgtggcgct tgacacgcac
     5221 cttcgggctc tctatctccc gccatctcgg aacgcacggg ggtgtagctc agttggttag
     5281 agcgctggcc tgtcacgcca gaggccgcgg gttcgagtcc cgtcactccc gccatcaccc
     5341 cgtttccggc cgcgccagcg gcaacctcgc agcaccggtc gacccgccgg gcgtgtgaca
     5401 cttggcgcgc tgccagggca tgaccgaaca tgccggccgg gaccggcgat gcttggcaag
     5461 ggcggcgcgg cggcctagtt tgcagcgcaa cgagaggcgg ccggccagcg tgccgtctgc
     5521 ccgcacacct cgttgcacgg gatgccgatg gacgcctatc tcctcgaata cctgccgatc
     5581 ctgatcttcc tcgccatcgc catcggcctt gctgcggtga tggtcctggc atcgatggtg
     5641 gtggcccggc agaagccgaa taccgagaag ctcagcccct acgagtgtgg cttcgcggcg
     5701 ttcgaggatt cgcgcggccg gttcgaggtg cgcttctatc tggtggccat cctcttcatc
     5761 atcttcgatc tcgaggtggc cttcctcttc ccctgggccg tcagcctcgg cgagatcggg
     5821 ctgcacggct tctggtcgat gatgctgttc ctcgccgtgc tcaccgtcgg gttcatctac
     5881 gaatggaaga agggagctct ggaatgggag tgagcaggcc gctcgttcgc cccgccgagg
     5941 cgccagccca gcccggcccg atcagcctct acgaggacct gggcagggac ctgcaggaca
     6001 agggcttctt cgtcaccggc ttcaacgacc tcgtgaactg ggcgcgttcc ggcagcctct
     6061 ggccgatgac cttcggcctt gcctgctgcg ccgtcgagat gatgcacgcc gccgccagcc
     6121 gctacgatct cgatcggttc ggcgtcgtct tccggccgag ccccaggcaa tcggacgtga
     6181 tgatcgtggc cggcacgctc tgcaacaaga tggccccggc gctgcgcaag gtctacgacc
     6241 agatgccgga gccccgctac gtcatctcga tgggctcctg cgccaatggc ggcggctact
     6301 accactattc ctactcggtg gtccgcggct gcgaccggat cgtgccggtc gacatctacg
     6361 tgcccggctg cccgccgacg gccgaggcgc tggtctacgg catcctgcag ctgcagaaga
     6421 agatccgtcg aaccgggacc atcgagcgat gacggccgag cagcaagccg acctcaacga
     6481 gtccttgcag gatctgaaga agctcgtcga ggaccatctc ggcgacgacc tgatcctggc
     6541 gcagctcgcg catggcgagc tctcgatcac ggttgcgacc accgacctgc cccgcgccct
     6601 gctctggctg cgcgacgatc cgaacatgct cttcaaggag ctggtcgaca tctgcggcgt
     6661 cgactatccc gagcgcgagc agcggttcga cgtcgtctac cacctgctct cgatgcacca
     6721 caaccagcgg atccgggtgc gggtgacgac cgacgaggcc accccggtgc cctcgacgac
     6781 cgggctcttc cgctccgccg gctggttcga gcgggaggcc tgggacatgt acggcatcct
     6841 cttctccgac catccggatc tccgtcgcct gctgaccgac tacggcttcg aggggcaccc
     6901 gctccgcaag gatttcccgc tgaccggcta tgtcgagatg cgctacgacg acgagcagca
     6961 gcgggtggtc tacgagccgg tcaagctgcg ccaggatttc cgcaacttcg acttcctgag
     7021 cccctgggag ggggcgggtg gcctccttcc cggcgacgag aaggcagtgg atccggccgc
     7081 ctgagccgga tggcccctag aggaacctga ccgaggaacg catggccgaa gtcgatctcc
     7141 gcagcttcac cgtcaatttc ggaccgcagc acccggcggc gcacggcgtg ctgcggctgg
     7201 tgctcgagat gtccggcgag atcgtcgagc gcgccgaccc gcatatcggc cttttgcacc
     7261 gcggcaccga gaagctgatc gagtatcgcc cgtatctgca gaacattccg tacttcgatc
     7321 gtctcgacta ctgctcgatg ctctgccagg agcatgccta cgtgctggcc gtcgagaagc
     7381 tgctcgaggt cgaggtgccg gagcgagcgc tctatatccg cacgctcttc gacgagatca
     7441 cccgcattct caaccatctg ctcaacatca cgacgatggc gctcgactgc ggcgcgatga
     7501 cgccgctgct ctggatgttc gaggagcgcg agaagctgat ggcgttctac gagcgggtca
     7561 gcggggcccg gatgcacgcc aactactacc gcttcggcgg cgtcgcgcgc gacatgccgg
     7621 ccgggctggc cgaggacatc gccacctggc tcgacagctt cgagcctttg ttcgaggatg
     7681 tcgacaacct cctgtccgag aaccggatct tcaagcagcg caccgtcgac atcggcgtgg
     7741 tcacggccga ggacgccttc aacctcggct tctccggccc gatgctgcgc gcttccggcg
     7801 tggcctggga cctgcgcaag gccgagcctt acgccatgta cgaccggatg gagttcgaca
     7861 tccccgtcgg ccggaacggc gactgctggg accgctacct ggtgcgcatg ctggaagtga
     7921 aggagtcgat gaagatcgtc cggcagtgcc tggaccagat gcggccgggg ccggtgcgca
     7981 ccgacgacaa gaaggtcaca ccaccgtcgc gcgcgaacat gaagcgctcg atggaggcgc
     8041 tgatccatca cttcaagctc tacaccgagg gcgtgcacgt gccggagggc gaggtctacg
     8101 ccgcggtcga gagcggcaag ggcgagttcg gcgtctatct cgtctcggac ggcaccaacc
     8161 ggccgtaccg gtgcaagatc cgctcgcccg gctacgtcca cctccaggcg ctggacgaga
     8221 tgtccaaggg tcaccagctg gccgatgtcg tcgcgatcat cggctcgatg gacattgtgt
     8281 tcggagagat cgaccggtga gcttttcgcc cgacatcgca accttcagct tcgccccgga
     8341 gctcggcgcc gagatcgagc ggatcatggc gcgctatccc gaggcgagga agcagagcgc
     8401 cgtcatgccg ctgctccatc tggcgcagaa gcagcacggc ggctggctgc cgcgcgcggc
     8461 actcgaccat gtggcggact tcctcgagat cccgcggatc agggtctacg aggtcgccag
     8521 cttctacgac atgtacaata ccgagccgac cggccggatc caggtgcggg tctgcacgac
     8581 gaccccctgc tggctctgcg gctccgacga cgtcgtccgg gcggccaagg aggtgctcgg
     8641 cgtcggcatt ggcgagagca ccgaggacgg ccggttcttc ctgcgcgagt tcgagtgcct
     8701 cggcgcctgc tgcaacgcgc cgatgatgtg gatcgatgac gactactacg aggatctgac
     8761 ctacgagcgg
//
LOCUS       DAOTFI010000208         8749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9619_length_8749_cov_8.707269, whole genome shotgun sequence.
ACCESSION   DAOTFI010000208 DAOTFI010000000
VERSION     DAOTFI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9619_length_8749_cov_8.707269"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="P5558_18300"
     CDS             complement(<1..1862)
                     /locus_tag="P5558_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRY26327.1"
                     /translation="MAEAKTARKRIRKSFGRITEVTTMPNLIEVQRQSYEFFLQMHTV
                     PGDRELAGLEEVFRSVFPIKDFADRAALDFVKYELEAPKYDVDECHQRGLTYAAPLRV
                     TLRLIVWDIDEDSGARSVRDIKEQDVYMGDMPLMTDNGTFVVNGTERVIVSQMHRSPG
                     VFFDHDRGKTHSSGKFLYSARVIPYRGSWLDFEFDAKDLVYVRIDRRRKLPATTLLLA
                     LGLDAESLLAHFYHEVAIQRCGQGWKIALRPDRLLGQKLTSDLVDAASGESLAEAGTK
                     VSPRLLKRIEEKKVTDLYLSAEDVVGHYIARDLVNMETGLVNAEAGEEITGELLGRLA
                     ELGIDELPLLDIDHSTIGPYIRNTLVLDRSHTREEALLDIYRVLRPGEPPTPETAEAL
                     FAGLFFDPERYDLSPVGRVKMNARLGLETDDTVRVLRNEDILAVVKHLVEIKDGRGEI
                     DDIDNLGNRRVRSVGELMENQFRVGLLRMERAVRERMSSVEIDAAMPHDLVNAKPVAA
                     SVREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRI
                     CPIETPEGPNIGLINSLATYARINKYGFIESPYRRVVDGKVTDEVIYLSAMEENR"
     gene            complement(2097..2477)
                     /gene="rplL"
                     /locus_tag="P5558_18305"
     CDS             complement(2097..2477)
                     /gene="rplL"
                     /locus_tag="P5558_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRY26328.1"
                     /translation="MANLQQLVDDLSSLTVLEAAELSKLLEEKWGVSAAAPVAVAAAG
                     GGAAVAAEAAEEQTEFDVILAAIGEKKINVIKEVRAITGLGLKEAKDLVEAAPKAVKE
                     GVSKDDAAKFKKQLEEAGATVEVK"
     gene            complement(2548..3057)
                     /gene="rplJ"
                     /locus_tag="P5558_18310"
     CDS             complement(2548..3057)
                     /gene="rplJ"
                     /locus_tag="P5558_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRY26329.1"
                     /translation="MLRAEKTRIVDELGSIFKEAEVVVVTHYMGMTVAEVTDLRRQMR
                     DVGASFRVTKNRLAKLAVVDTAYAPLGELLTGPTAIAYSPDPVAAPKVIAAFAKKNDK
                     LKIVGGGYGGNLLDAAAVQTLAELPSLDELRAKLLSLINTPATRLVGLLQAPGGQIAR
                     VLKAKAELG"
     gene            complement(3286..3999)
                     /gene="rplA"
                     /locus_tag="P5558_18315"
     CDS             complement(3286..3999)
                     /gene="rplA"
                     /locus_tag="P5558_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRY26330.1"
                     /translation="MARTGKRLKAAASGIDRQKQYELGEAITAIKGAATAKFDETIEM
                     SLNLGVDPRHADQMVRGVVNLPHGTGKTLRVAVFAKGDKAEQALAAGADIVGADDLAE
                     KVQAGQIDFDRVIATPDMMPLVGRLGRILGPRGLMPNPKLGTVTPNVTEAVEAAKGGQ
                     VQFRVEKAGIVHAGIGRASFAAEQLAENARSFVDAVNRAKPAGAKGTYLKRAHLSSTM
                     GPGVKLEVASLVGANPAGQ"
     gene            complement(4004..4435)
                     /gene="rplK"
                     /locus_tag="P5558_18320"
     CDS             complement(4004..4435)
                     /gene="rplK"
                     /locus_tag="P5558_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRY26331.1"
                     /translation="MAKKITGYIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGMPIPTVITAFGDRTFTFVTKTPPASYFLKKAAGLGKGGQTPGKAVAGKVT
                     VAQCREIAESKMKDLNAADLDAATKMIVGSARSMGLDVVEG"
     gene            complement(4521..5051)
                     /gene="nusG"
                     /locus_tag="P5558_18325"
     CDS             complement(4521..5051)
                     /gene="nusG"
                     /locus_tag="P5558_18325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRY26332.1"
                     /translation="MAARWYIIHVYSTFEQKVKEAILEQAEQKGLVKLIEDILVPSEE
                     VVEVRRGKKVATERKFFPGYVLIKMELNDETWSLVRNTAKVTGFLGAGGKPQAIPDAE
                     AERIQAQVQEGVEHPRMSVSFVSGDRIRVTDGPFSTFEGTVEDVEEGKGRLKVSVSIF
                     GRSTPVELEFSQVERI"
     gene            complement(5051..5248)
                     /gene="secE"
                     /locus_tag="P5558_18330"
     CDS             complement(5051..5248)
                     /gene="secE"
                     /locus_tag="P5558_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRY26333.1"
                     /translation="MAKTSPAQFVREVRQEISKVTWPTRKELIVTTISVLVMSALAAL
                     FFFLVDQVIAFSIKFLLGLGG"
     gene            complement(5284..5359)
                     /locus_tag="P5558_18335"
     tRNA            complement(5284..5359)
                     /locus_tag="P5558_18335"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5324..5326),aa:Trp,seq:cca)
     gene            complement(5528..6718)
                     /gene="tuf"
                     /locus_tag="P5558_18340"
     CDS             complement(5528..6718)
                     /gene="tuf"
                     /locus_tag="P5558_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRY26334.1"
                     /translation="MAKEKFQRDKPHANIGTIGHVDHGKTTLTAAITKVLAETGGATF
                     TSYDNIDKAPEEKARGITINTAHVEYQTKDRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDPELLELVELEVRELLSS
                     YEFPGDDIPIIAGSALAALDGSNNELGHDKVLELMAAVDSYIPQPERAIDQAFLMPIE
                     DVFSISGRGTVVTGRIERGIVKVGDEVEIVGIKDTTKTTCTGVEMFRKLLDRGEAGDN
                     VGVLLRGTKRDDVERGQVLCKPGSIKPYTKFKAETYVLTKEEGGRHTPFFTNYRPQFY
                     FRTTDVTGMVHLPEGTEMVMPGDNVTMSVELIAPIAMEQGLRFAIREGGRTVGAGVVA
                     EIIS"
     gene            complement(6750..>8749)
                     /gene="fusA"
                     /locus_tag="P5558_18345"
     CDS             complement(6750..>8749)
                     /gene="fusA"
                     /locus_tag="P5558_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY26335.1"
                     /translation="TERILYYTGKSHKIGEVHDGAATMDWMEQEQERGITITSAATTC
                     FWNDHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFDSVAGVEPQSETVWRQADKYRV
                     PRMCFINKMDRTGANFDRCVQMMRDRLGAHPVIINLPIGVEADFKGVADLINNRAIIW
                     HDESLGAEFSYSEIPAALADKAAEARAALIEAAVEFDDAAMEAYLEGEEPDPATLVAC
                     IRKGTVAGKLTPVLCGSAFKNKGVQPLLDAVIDFLPSPVDVPPTEGLKPDSDEVLTRL
                     PRDSEPFAALAFKIMTDPFVGTLTFARIYSGQVESGSYVQNTVKNKRERVGRLLLMHA
                     NSREEIKDAQAGDIVAFVGLKDTTTGDTLSDQAKPIVLERMEFPEPVIEVAVEPKTKA
                     DQEKMSIALNKLAQEDPSFRVGVDEESGQTVIKGMGELHLEILVDRMKREFKVDANVG
                     APQVAYRETITRTSEIDYTHKKQTGGSGQFARIKLTFEPGEKASGFKFESKVVGGSVP
                     KEYIPGVQKGLSQSMSNGVIAGFPMTDVTVKLIDGAYHDVDSSVMAFEIAARAAFKEG
                     IAKAGPQLLEPMMRVEVVTPDEYMGDIIGDLNSRRGQVTGMEPRGNGQVVNAMVPLAT
                     MFGYVNTLRSLSQGRAQYTMHFDHYDAVPQMVAEEIRAKYA"
BASE COUNT         1324 a         3034 c         2798 g         1593 t
ORIGIN      
        1 cggttctcct ccatggccga gaggtagatc acctcgtcgg tcaccttgcc gtcgacgacc
       61 cggcgatacg ggctctcgat gaagccgtac ttgttgatcc gggcataggt ggcgaggctg
      121 ttgatcaacc cgatgttcgg gccttccggc gtctcgatcg ggcagatgcg gccataatgc
      181 gtcgggtgca cgtcgcgcac ctcgaagccg gcccgctcgc gggtcaggcc acccgggccg
      241 agcgccgaca ggcgccgctt gtgggtgatc tccgagagcg ggttggtctg gtccatgaac
      301 tgcgagagct gcgagctgcc gaagaactcg cggaccgagg ccgccaccgg cttggcgttg
      361 accaggtcgt gcggcatggc ggcgtcgatc tcgaccgagc tcatccgctc acggacggcg
      421 cgctccatgc gcaagaggcc gacgcggaac tggttctcca tgagctcgcc gaccgagcgc
      481 acccggcggt tgccgagatt gtcgatgtcg tcgatctcgc cgcggccgtc cttgatctcg
      541 accaggtgct tgaccacggc gaggatgtcc tcgttgcgca gcacgcgcac cgtgtcgtcc
      601 gtctccaggc cgagcctggc gttcatcttg acgcgaccga ccggcgagag gtcgtagcgc
      661 tcgggatcga agaagaggcc ggcgaagagc gcctcggccg tctccggcgt cggcggctcg
      721 ccagggcgca gcacccggta gatgtcgagc agggcctcct cgcgggtatg cgagcggtcg
      781 agcaccagcg tgttgcggat gtaggggccg attgtcgagt ggtcgatgtc gagcagcggc
      841 agctcgtcga tgccgagctc ggcgaggcgg ccgagcagct cgccggtgat ctcctcgccg
      901 gcctcggcgt tgaccaggcc ggtctccatg ttgacgaggt cgcgtgcgat gtagtggccg
      961 accacgtcct cggcggagag atagaggtcg gtgaccttct tctcctcgat ccgcttcagg
     1021 agcctgggac tcaccttggt cccggcctcg gcgagcgact cgccggaggc ggcgtcgacc
     1081 aggtcgctgg tcagcttctg accgagcaga cggtcggggc ggagcgcgat cttccagccc
     1141 tggccgcagc gctggatggc gacctcgtgg tagaaatgcg ccagcagcga ttcggcgtcg
     1201 aggccaagcg ccagcagcag cgtcgtcgcc ggcagcttgc gccgccggtc gatgcgcaca
     1261 tagaccagat ccttggcgtc gaactcgaaa tcgagccacg agccgcgata ggggatgacc
     1321 cgggccgagt agaggaactt gcccgagctg tgcgtcttgc cgcggtcgtg gtcgaagaag
     1381 acgcccggcg agcggtgcat ctggctgacg atgacgcgct ccgtgccgtt gacgacgaag
     1441 gtcccgttgt ccgtcatgag cggcatgtcg cccatgtaga cgtcctgctc cttgatgtcg
     1501 cgcacactgc gcgcgccgct gtcctcgtcg atgtcccaga cgatcaggcg cagcgtcacc
     1561 cgcagcggcg ccgcgtaggt caggccgcgc tggtggcact cgtcgacatc gtatttcggc
     1621 gcctcgagct cgtacttgac gaagtcgagc gccgcccggt cggcgaagtc cttgatcgga
     1681 aagaccgaac ggaagacctc ctcgagcccg gccagctccc ggtcgccagg caccgtatgc
     1741 atctgcagga agaactcgta actctgccgc tgcacctcga tgaggttggg catcgtcgtg
     1801 acttccgtaa tacgaccgaa gctcttgcgg atccgcttac gcgccgtctt cgcctctgcc
     1861 atcactgact tccgtttccc atgtcgggcc gccggtccac ccggcgccca cgtcgccggg
     1921 ccgcctcagc ggaccggcgc aacgacagaa aggcggaccg caacccccgg ctgcagccgc
     1981 cttccctgtt cagatattca tgcgacgttc gaatgcgccg aaggcacaca gcatcgttcc
     2041 gggcctttcg gcccggtccg gatgcgcgcc ggacgctcgc cgcgcctgtg tcgatcttac
     2101 ttgacctcca cggtggcgcc ggcctcctcg agctgcttct tgaacttcgc tgcgtcatcc
     2161 ttggagacgc cttccttgac cgccttcggc gccgcctcga ccagatcctt ggcctccttg
     2221 aggccgaggc cggtgatcgc ccggacctcc ttgatgacgt tgatcttctt ctcgccgatc
     2281 gcggcgagga tcacgtcgaa ctcggtctgc tcctcggccg cctcggcggc gacggcagcg
     2341 ccaccgccag cggcggccac ggcgacgggg gccgcggccg agacgcccca cttctcctcg
     2401 agcaacttcg agagctcggc ggcctcgagc acggtcaggc tcgagagatc gtccaccagc
     2461 tgctgcagat tagccatttc ggtcatcctc caacgggtct tgcaaggtcg ggcggcccaa
     2521 ccatcgggcc acggcaatcg gttcggttca gcccagctcg gccttggcct tgagcacgcg
     2581 ggcgatctgg ccgccgggtg cctgcagcag gccgacgagg cgggtcgcag gggtgttgat
     2641 cagggacagg agcttggcgc ggagctcgtc gagcgacggc aactcggcca gggtctgcac
     2701 ggcggcggca tcgagcagat tgccgccata gccgccaccc acgatcttca gcttgtcgtt
     2761 cttcttggcg aaggcggcga tcaccttcgg cgctgcgacg gggtcgggcg agtaggcgat
     2821 cgccgtcggg ccggtgagca gctcgccgag cggcgcgtaa gcggtgtcga ccacggccag
     2881 cttcgccagg cggttcttgg tcacccggaa gctggcgccg acgtcgcgca tctgccgccg
     2941 gagatcggtc acctccgcca cggtcatgcc catgtagtgc gtcacgacga cgacttcggc
     3001 ttccttgaag atggaaccga gttcatcgac gatcctggtt ttctcggcgc ggagcacgga
     3061 cggcctccaa atttcagcag gaacgcgggc acgcacggcg acccatggcc accgctgcgc
     3121 cgcccgccgg cacgagtgag acgaaggcga caaatagccc acgcgtgacc ccactcatcc
     3181 gatcggatga atggggggaa cccgtagacc cgctgttctc aggagcgcgc agcatagccg
     3241 ccttgtccac tctcgcaagc agtttcttta tcggtgccgg gcggctcact ggcctgccgg
     3301 gttggcgccg accaggctgg ccacctcgag cttcacaccc gggcccatgg tcgagctcag
     3361 atgggcgcgc ttgaggtagg tgcccttggc acccgccggc ttcgcccggt tcaccgcatc
     3421 gacgaagctc ctggcgttct cggcgagctg ctcggcggcg aagctcgccc ggccgatgcc
     3481 ggcatggacg atgcccgcct tctcgacccg gaactgcacc tggccgccct tggccgcctc
     3541 gaccgcttcc gtgacgttgg gcgtcacggt gccgagcttc gggttcggca tcaggccgcg
     3601 cgggccgaga atgcggccga gccggccgac cagcggcatc atgtccggcg tcgcgatcac
     3661 ccggtcgaag tcgatctgcc cggcctgcac cttctcggcg aggtcgtcgg cgccgacgat
     3721 gtcggccccg gctgccaggg cctgctcggc cttgtcaccc ttggcgaaga ccgcgacacg
     3781 cagcgtcttg ccggtgccgt gcggcagatt gaccacgcca cgcaccatct ggtcggcatg
     3841 gcgcgggtcg accccgagat tgagcgacat ctcgatcgtc tcgtcgaact tggcggtcgc
     3901 cgcccccttg atcgcggtga tcgcctcgcc gagctcgtac tgcttttgac ggtcgatgcc
     3961 ggatgccgcc gccttcagtc gcttgcctgt cctggccatc ggctcagccc tccaccacgt
     4021 cgaggcccat cgatcgggcc gagccgacga tcatcttcgt cgcggcatcg agatcggcgg
     4081 cgttgaggtc cttcatcttg ctctcggcga tctcgcggca ctgcgccacg gtcaccttgc
     4141 cggcaacggc cttgcccggg gtctgcccgc ccttgccgag gccggccgcc ttcttcagga
     4201 agtagctggc gggcggcgtc ttggtgacga aggtgaaggt gcggtcgccg aaggcggtga
     4261 tcaccgtcgg gatcggcatg ccgggctcca tgccctgcgt cgccgcgttg aacgccttgc
     4321 agaactccat gatgttcagg ccgcgctgac ccagagccgg cccgatgggc ggcgacgggt
     4381 tggccttgcc ggccggcacc tggagcttga tgtagccggt gatcttcttc gccattcaca
     4441 gcactcctct tcgcggtgca tgcgcgccgc tgccgcgacg ctcccgctgc aacgccggcc
     4501 cccctggccg gcgacgtgtc ctagatgcgc tcgacctggg agaattccag ctcgaccggc
     4561 gtggaccggc cgaagatcga gacggaaacc ttgagccggc ccttgccttc ctcgacgtcc
     4621 tcgacggtgc cctcgaaggt gctgaacggc ccgtcggtga cccggatgcg atcgccgctg
     4681 acgaacgaga cggacatgcg cgggtgctcg acgccctctt gcacctgggc ctggatccgc
     4741 tccgcctcgg catcgggtat cgcctgcggc ttgccgccgg caccgaggaa gcccgtcacc
     4801 ttggcggtgt tgcgcacgag cgaccaggtc tcgtcgttga gctccatctt gatcagcacg
     4861 tagccgggga agaacttgcg ctcggtggcc accttcttgc cgcggcgcac ctcgacgacc
     4921 tcttccgagg gaacgaggat gtcctcgatc agcttgacga ggcccttctg ctccgcctgc
     4981 tcgagaatcg cctccttcac cttctgctcg aaggtggagt agacgtgaat gatgtaccag
     5041 cgcgccgcca tcagccgccg agccccagga gaaacttgat gctgaaggcg atcacctgat
     5101 ccaccagaaa gaagaaaagc gccgccaggg ccgacatgac gagcacgctg atcgtggtga
     5161 cgatcagctc cttgcgggtc ggccaggtga ccttgctgat ctcctgcctg acctctcgaa
     5221 cgaactgcgc gggcgacgtc ttcgccatct ggatccactc tgatttcagc taggcgggcc
     5281 gactggcagg agtggaggga ctcgaaccca caacccccgg ttttggagac cggtgctctg
     5341 ccaattgagc tacactcctc cggcggctcg ccgggtcgac atcgcccagg atcgaggacg
     5401 acagcccgcg ccggcggcac gtgccgccgg gcgagcccgt gtctctaagg cgtcaaccgc
     5461 gaaagggcaa gccccttgct gtcggccgcc ggagagaacg cgctcgcccg ctgacccgct
     5521 atcggtttca gctgatgatt tcggcgacga cgccggcgcc gacggtgcgg ccgccctcgc
     5581 ggatggcgaa gcgcaggccc tgctccatgg cgatgggggc gatcagctcg accgacatcg
     5641 tcacgttgtc acccggcatc accatctccg tgccctcggg caggtggacc atgccggtca
     5701 cgtccgtcgt ccggaagtag aactgcggcc ggtagttggt gaagaacggc gtgtgccgcc
     5761 cgccctcctc cttggtcagc acgtaggtct cggccttgaa cttcgtgtac ggcttgatcg
     5821 agcccggctt gcacagcacc tggccgcgct ccacgtcgtc gcgcttggtg ccgcgcagga
     5881 gcacgcccac gttgtcgccc gcctcgccgc gatccaaaag cttcctgaac atctccacgc
     5941 ccgtgcaggt cgtcttggtc gtgtccttga tgccgacgat ctcgacctcg tcgcccacct
     6001 tgacgatgcc ccgctcgatc cgccccgtca ccaccgtgcc gcggcccgag atcgagaaca
     6061 cgtcctcgat cggcatcagg aaggcctggt cgatcgcccg ctccggctgc ggaatgtagc
     6121 tgtccaccgc cgccatcagc tccagcacct tgtcgtgacc gagctcgttg ttcgagccgt
     6181 ccagcgccgc cagcgccgag cccgcgatga tcggaatgtc gtcgcccggg aactcgtacg
     6241 agctcagcag ctcgcgcacc tcgagctcga ccagctccag aagctccggg tcgtcgacca
     6301 tgtcgacctt gttcaaaaac accaccagcg ccggcacgcc cacctgccgc gccagcagga
     6361 tgtgctccct ggtctgcggc atcggcccgt cagccgccga aaccaccaga atggcaccgt
     6421 ccatctgcgc cgcgcccgtg atcatgttct tcacgtagtc ggcgtggccc gggcaatcga
     6481 catgcgcgta atgccggtcc ttcgtctggt actcgacatg cgccgtgttg atcgtgatcc
     6541 cccgcgcctt ctcctccggc gccttgtcga tgttgtcgta cgacgtgaac gtcgccccgc
     6601 ccgtctccgc caggaccttc gtgatcgccg ccgtcagcgt cgtcttgccg tggtcaacgt
     6661 gaccaatcgt accaatgttc gcgtgcggct tgtcccgctg aaacttttcc ttggccatcg
     6721 cccagtctcc gtcctgtcca gcgcctcgtt caggcgtatt tcgcgcgaat ttcctcggcc
     6781 accatctgcg gcaccgcatc gtagtggtcg aaatgcatgg tgtactgcgc ccgcccctgc
     6841 gacagcgacc gcagggtgtt gacgtaaccg aacatcgtcg ccagcggcac catggcgttg
     6901 accacctggc cgttgccacg cggctccatg ccggtgacct ggccgcgccg gctgttcagg
     6961 tcgccgatga tgtcgcccat gtactcgtcg ggcgtcacca cctcgactcg catcatgggc
     7021 tcgagcaact gcgggccagc cttggcgatg ccctccttga aggcggccct ggccgcgatc
     7081 tcgaaggcca tcaccgagct gtcgacgtcg tggtaggcgc cgtcgatcag cttcaccgtc
     7141 acgtcggtca tcgggaagcc agcgatgacg ccgttcgaca tcgactgtga caggcccttc
     7201 tgcacgcccg ggatgtactc cttgggcacc gagccaccga cgaccttgct ctcgaacttg
     7261 aagcccgaag ccttctcgcc cggctcgaac gtcagcttga tccgggcgaa ctggcccgag
     7321 ccgccggtct gcttcttgtg ggtgtagtcg atctcggacg tcctggtgat cgtctcgcga
     7381 taggcgacct gcggggcgcc gacattcgca tccaccttga actcgcgctt catccggtcg
     7441 accaggatct cgagatggag ctcgcccatc cccttgatca cagtctggcc gctctcctcg
     7501 tcgacgccga cccggaacga cggatcctcc tgcgccagct tgttgagcgc gatcgacatc
     7561 ttctcctggt cggccttggt cttcggctcg accgcgacct cgatcaccgg ctccgggaac
     7621 tccatccgct cgagcacgat cggcttcgcc tgatcgctga gcgtgtcacc ggtggtcgtg
     7681 tccttgagcc cgacgaaggc gacgatgtcg ccggcctggg cgtccttgat ctcctcgcgg
     7741 ctgttggcgt gcatcaacag caggcggccg acgcgctcgc gcttgttctt gaccgtgttc
     7801 tgcacatagg agccgctctc gacctggccc gagtagatgc gggcgaaggt cagcgtgccg
     7861 acgaagggat cggtcatgat cttgaaggcg agcgcggcga acggctcgct gtcgcgcggc
     7921 aggcgggtca gcacctcgtc gctgtccggc ttcaggccct cggtcggcgg cacgtcgacc
     7981 ggcgacggca ggaagtcgat gaccgcgtcg agcagcggct gcacaccctt gttcttgaag
     8041 gcggagccgc agagcaccgg cgtcagcttg ccggccaccg tccccttgcg aatgcaggcg
     8101 acgagggtcg ccggatccgg ctcctcaccc tcgagatagg cttccatggc ggcgtcatcg
     8161 aactcgaccg cggcctcgat cagggcagcg cgcgcctcgg ctgccttgtc ggccagcgcg
     8221 gccgggatct cactgtagga gaactcggcg cccaggctct cgtcgtgcca gatgatcgcc
     8281 cggttgttga tcaggtcggc cacgcccttg aagtcggcct cgacgccgat cggcaggttg
     8341 atgatcaccg ggtgggcacc cagccggtca cgcatcatct gcacgcaccg atcgaagttc
     8401 gcgccggtgc gatccatctt gttgatgaag cacatgcgcg gcacgcgata cttgtcggcc
     8461 tgacgccaga ccgtctcgga ctgcggctcg acaccagcca cgctgtcgaa cacggcaacg
     8521 gcaccgtcga gcacacgcag gctgcgctcg acctcgatgg tgaagtcgac gtggcccggg
     8581 gtgtcgatga tgttgatccg gtggtcgttc cagaagcacg tcgtagccgc cgaggtgatc
     8641 gtgatccccc gctcctgctc ctgctccatc cagtccatgg tcgcggcgcc gtcgtgcacc
     8701 tcgccgatct tgtgcgactt gccggtgtaa tagaggatgc gctccgtgg
//
LOCUS       DAOTFI010000209         8739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9632_length_8739_cov_8.165592, whole genome shotgun sequence.
ACCESSION   DAOTFI010000209 DAOTFI010000000
VERSION     DAOTFI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9632_length_8739_cov_8.165592"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="P5558_18350"
     CDS             <1..879
                     /locus_tag="P5558_18350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY26336.1"
                     /translation="RANVWLFLLPALVFFVGYQVWPIIRVVWISFTDYQFLANEPANL
                     VGFANYAEALTDPLMWAGLGRAALFTAMFLPGTIILPLLLAILVDRVRNQRVATVYRV
                     IILIPAVIPSTLIFVLWKWMYNFQVGPINHFLVEVLGVFTIQTAPQWLGGTALTLPSI
                     AIMEVWWGLGYHTVFFLAGLAAIPKELTEAARVDGANEWQIFWNVTLPALQPIMMILI
                     VLRFGTSMAVIDEYLIFGGFDRESPTYTWTIFMYDQAFKTGLWRQGFAAAIGMIGAVF
                     MMVFVGGLLYLFRRKG"
     gene            885..1754
                     /locus_tag="P5558_18355"
     CDS             885..1754
                     /locus_tag="P5558_18355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY26337.1"
                     /translation="MATATAAIPMQRNPVLHRAAVIRHCIILFFCAIIILPLLWVLLL
                     SIKSMPDSMRGNLWPRNFDFTHYGYVIEKIDTLPINLFNSVFVTGFTVVITCFCAVLA
                     GYALVHLKFGGKAWIIAMLIASLYFPTRVVSIITIYGIQSDLGLINSTAGLILPYITL
                     SLAVSILIMRSVFQLVPGELFDAAKIDGAGPWKCLWMVGLPLVRNGLVVIIIVNFVTA
                     WGEYLLCSTLTNDQSARTVPVMLAHAAGGLGQWAWPRIAAVYVIVTAPGLIAFAFAQK
                     LFFKGLMEGGIKA"
     gene            complement(2061..2588)
                     /locus_tag="P5558_18360"
     CDS             complement(2061..2588)
                     /locus_tag="P5558_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26338.1"
                     /translation="MRTPFIIAAVVSAFAATASAAPFTTPYVLSAVHADFLTQLEKVA
                     ETPGEIGTAARAAADLLAAHNEAEERVALPLLGLTEEVAEGKAVDDPEATLASLESLQ
                     AELPLLVEGQAEAIGALVKLFAAAEAEGEADVSRLAERIIWHELTDAEILYSAATLVG
                     ATVQAGMADADVEKN"
     gene            complement(2758..3027)
                     /locus_tag="P5558_18365"
     CDS             complement(2758..3027)
                     /locus_tag="P5558_18365"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRY26339.1"
                     /translation="MRKTLAILAFFAVPPAGAALADDDCTVPMAEWQPREAVQQMADA
                     QGWAVRRIKIDDGCYEIKGRDAAGRAIKVKIDPATLTIVELELDD"
     gene            3174..3833
                     /locus_tag="P5558_18370"
     CDS             3174..3833
                     /locus_tag="P5558_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY26340.1"
                     /translation="MRLLLIEDDTVLGSAVRDQIAGDGQSVDWVTRLDAAGDALASAA
                     YDLILLDLMLPDGRGIGFLKALRVRGDVTPVIILTALDQVSDRIEGLNAGADDYLVKP
                     FDLAELSARIGSVARRYTGNPNPIITHGALDIDLAARSIRRAGRPIQLTAREWALFET
                     FLARPGQLLSKAQLEEKLYAFDAEVESNTIEVHVSRLRKKLGAGVIETERGLGYRLGT
                     P"
     gene            3830..5200
                     /locus_tag="P5558_18375"
     CDS             3830..5200
                     /locus_tag="P5558_18375"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY26341.1"
                     /translation="MRLPRSLQARLGISIGALLTLLWIAAASVTAIVLRHEMQEVFDS
                     TLQETAQRLLPLAVLDIVDREADDDAQRLAQIREHDEHFTYVVRDALGQILLQSHTAD
                     PLIFPTYDGPGFAQTATHRFYSEEALRGTVRITVAEPMEHRAAIARDIQMGLGLPVLV
                     VIPVALLAVVLAVRANLAPLRRFRERLEARHARDLSPVPARDLPAEIAPIAATLSGLL
                     DRLKAAFDAERNFAANAAHELRTPLAGAIAQAQRLRSETADPQAERRAGEIEATLKRL
                     TRLAERLMQLARAEGGRLRLDRSSDLRLVARVVVDDIDRTPTRDRVTLTLAGRPVMSD
                     LDPDAFGILCRNLVDNALRHGAATSPVEVTLSESGLLTVANEGPVVPAETLSRLTTRF
                     ERASQHTSGSGLGLAIAAAIADRIGSRLVLRSPRPGKSSGFEAAVTLPTSASPAASIA
                     GADAKG"
     gene            5375..6517
                     /locus_tag="P5558_18380"
     CDS             5375..6517
                     /locus_tag="P5558_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRY26342.1"
                     /translation="MNDTPDADWDPRSHQVQSDQRAAYDAMRETCPVAWSDYMQWSLF
                     RNADVTRAVLDHATFSNAVSNRLTVPNGMDPPEHTAWRPIVEKYFTRSRVDAFEPVCR
                     GIAAALIAAAAGPVELMGAIAHPFAVRVQCAFLGWPDELAEPLRRWVARNHEATLARD
                     HGAMTRIAGEFQDLIRDTIAARIAVGATPETDVAVALTQETIHGRRLNEDEITSILRN
                     WTVGEIGTIAASVGILAEYLASDVGLQGRLRADPSLAGPAADEILRLHGPLVTNRRVT
                     TCPVEIGGRRLPAGARVTVNWVAANRDPRVFEDPDVFRLDRDPSDNLLYGAGIHVCPG
                     AQLARMELRVFLEELLARTSAIAPHRPATPAAYPASGFRTLHLLIS"
     gene            complement(6579..7601)
                     /locus_tag="P5558_18385"
     CDS             complement(6579..7601)
                     /locus_tag="P5558_18385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY26343.1"
                     /translation="MEPETPTGDAPILVTGATGFAGGHLALRLRKDGHKVRALVRDGA
                     RVDHLKAAGVELAQGDIRHAAEVDKAVAGCAKVYHIAAVFRTASHPDSYYEDVNVGGT
                     VNVLEAARKHGVERTIHCSTIGVHGDVQEFPCTETSPFNPGDIYQRTKLEGEMKAQEA
                     FKAGLPGVVFRPAGMYGPGDLRFLKLFRSVNNGRFVMFGSGETFYHLVYADDLIDGIL
                     LCGTHPAALGGTFIIAGDRWVSLNDWVGEIAKALDVPPPKLRLPFWTLSAGSALCEAL
                     CKPLGIEPPLHRRRASFFIKHRAFSTERARTVLGYAPKVPLEEGIRRTAAWYIENGHI
                     TRRRAA"
     gene            complement(7591..>8739)
                     /locus_tag="P5558_18390"
     CDS             complement(7591..>8739)
                     /locus_tag="P5558_18390"
                     /inference="COORDINATES: protein motif:HMM:NF028211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRY26344.1"
                     /translation="MDAAAEGAVVANHVEALGFLGTPERVTGARARDRLGDEEFDIRA
                     KLTINAAGPHAGLLLDRLPARSGRDFGGLARGLNIVVRRPLATGHALAVSSRRRSDSL
                     VPRADGRLFFIQPWQGLTLIGTSHLPHADDSPELVVDGPWLDDFLAEINDAWPPAALT
                     REDVLYCYAGLTPAAKEARGSEVARTRRGVVIDHGPADGIQGVLSLQGVKYTTARLVA
                     ESVVDLALARLGRPAVPCRSWQEQLPGARGFEAGAALAAARRRAPGGGREIDRLVADH
                     GAGWAAVLDDAGEGPLTGETVFRARVRHVVRHEMACRLDDVLVRRLDEVARGRLDRAR
                     LDAAAGVMAEELGWSPAERAAAVADLARHGPAFGHHPQTTKDIADHGA"
BASE COUNT         1280 a         3177 c         2864 g         1418 t
ORIGIN      
        1 cgggccaatg tctggctctt cctcctgccg gcgctcgtct tcttcgtcgg ctaccaggtc
       61 tggcccatca tccgggtcgt ctggatcagc ttcaccgact accagttcct cgccaacgag
      121 ccggccaacc tggtcggctt cgccaactat gccgaagccc tgaccgatcc cctgatgtgg
      181 gccgggctcg ggcgggctgc cctgttcacg gcgatgttcc tgccgggcac gatcatcctg
      241 ccgctgctcc tcgccatcct ggtggaccgg gtccggaacc agcgggtcgc caccgtctac
      301 cgggtcatca tcctgatccc ggcggtgatc ccgagcacgc tgatcttcgt gctctggaaa
      361 tggatgtaca atttccaggt cgggcccatc aatcacttcc tggtggaggt gctgggtgtg
      421 ttcaccatcc agaccgcccc ccagtggctc ggcggcacgg cgctgaccct gccgtccatc
      481 gccatcatgg aggtctggtg ggggctcggc taccacaccg tcttcttcct cgccggcctc
      541 gccgcgatcc ccaaggagct gaccgaggcg gcccgggtcg atggcgccaa cgagtggcag
      601 atcttctgga acgtcaccct gccggccctg cagccgatca tgatgatcct gatcgtgctg
      661 cgcttcggca cgtcgatggc ggtgatcgac gagtacctga tcttcggcgg cttcgaccgc
      721 gaatcgccga cctacacctg gacgatcttc atgtacgacc aggcgttcaa gaccgggctc
      781 tggcgccagg gcttcgccgc cgccatcggc atgatcggcg ccgtcttcat gatggtcttc
      841 gtcggcggcc tcctctacct cttccggcgc aagggctgaa gatcatggca accgcaaccg
      901 ccgccatccc gatgcagagg aacccggtgc tgcatcgggc cgccgtcatc cgccactgca
      961 tcatcctctt cttctgcgcc atcatcatcc tgcccctgct ctgggtgctg ctcctctcga
     1021 tcaagtcgat gccggattcg atgcgcggca atctctggcc acgcaatttc gacttcaccc
     1081 attacggcta cgtcatcgag aagatcgaca cgctgccgat caacctcttc aacagcgtct
     1141 tcgtcaccgg cttcacggtg gtcatcacct gcttctgcgc cgtgctcgcc ggctacgcgc
     1201 tggtccacct caagttcggc ggcaaggcct ggatcatcgc catgctgatc gcgtcgctct
     1261 acttcccgac gcgcgtcgtc tcgatcatca ccatctacgg catccagagc gacctcggcc
     1321 tgatcaacag cacggccggc ctgatcctgc cctacatcac gctgagcctc gccgtctcca
     1381 tcctgatcat gcgcagcgtc ttccagctcg tcccgggcga gctcttcgat gcggccaaga
     1441 tcgacggcgc cggcccctgg aagtgtctct ggatggtcgg cctgccgctg gtcaggaacg
     1501 gcctcgtcgt catcatcatc gtcaacttcg tcaccgcctg gggcgagtac ctgctctgct
     1561 cgaccctgac caacgaccag agcgcgcgca ccgtgccggt catgctggcg catgccgcgg
     1621 gcggcctcgg ccagtgggcc tggccgcgca tcgccgccgt ctacgtgatc gtcaccgccc
     1681 ccggcctcat cgccttcgcc ttcgcccaga agctcttctt caaggggttg atggagggtg
     1741 gcatcaaggc ctgagcgcgg gtcgagccag gcagtattcc cttcataggc ggagcgttgg
     1801 ccgattcccg atgatgttgc gggcgtcggc ggggcggggt tcactccacc attccagcca
     1861 tctcgctggc gaatatccgc tggggccggc ggggagatca gtctccccgc cggcttcatg
     1921 ccgggcaatc ctgagcccgc ggcacccgcc tcgcaagcat caccaacgct cgagcttctc
     1981 cccgggcgag ccgcgcgtgc gccgccggcc cgcgttcgcc atcgagatcg ggtcacggtc
     2041 ggagagcggc cccgatcgca ctagttcttc tccacatccg cgtcggccat gcccgcctgg
     2101 acggtggcgc cgacgagtgt cgcggctgaa tagagaatct cggcatcggt cagctcgtgc
     2161 cagatgatcc gctcggccag acgcgagaca tccgcctcgc cctccgcttc ggccgcggcg
     2221 aacagcttga ccagggcacc gatggcctcc gcctgcccct cgaccaggag aggcagctcc
     2281 gcctgcaggc tctccaggct cgccagcgtc gcctcgggat cgtccaccgc cttgccctcg
     2341 gcgacttcct cggtgagccc gaggagcggc agggccaccc tttcctcggc ctcgttgtgc
     2401 gccgccagaa ggtcggctgc ggcgcgggca gcggtgccga tctcgcccgg tgtctcggcg
     2461 accttctcga gctgcgtgag gaagtccgca tggaccgcgc tcagcacgta cggggtcgtg
     2521 aagggggcgg ccgatgccgt cgcggcgaac gcggacacga cagcggcaat gatgaacggc
     2581 gtgcgcatga tcttctccct tgattgaagc gtgggtgatc ctcgatttct cttcatggtc
     2641 cgggtctcct tcgagccgat gcgatcaatg tggagccgcc acctgaaggc ttgctgacag
     2701 cggcgctgtt ttcgcttcag ctcgacgtca ggaaggcagg caggatttgg cacgttctca
     2761 gtcgtcgagc tcgagctcga cgatcgtcag cgtcgcggga tcgatcttga ccttgatggc
     2821 gcgaccggcc gcatcgcggc ccttgatctc gtagcagccg tcatcgatct tgatgcgccg
     2881 cacggcccac ccctgcgcgt ccgccatctg ctgaaccgcc tcgcgcggct gccactcggc
     2941 catcggcacc gtgcagtcgt cgtcggccag cgccgccccg gccggcggga cggcgaaaaa
     3001 ggccagaatt gccagggtct tcctcatgcg cggggctccg tgttgcggcc atctcgcgcg
     3061 gagcatgcac cttgaagctg acggcagcct gaagcgtcgg cccggtgagc gtcagcctcg
     3121 cgtcaggccg gaaaggtata agaaggcacg cggaacaatt ggagccgaga gccatgcgcc
     3181 ttctgctgat cgaggacgac acggtcctcg gctccgccgt gcgcgaccag atcgccgggg
     3241 acggccaatc cgtcgactgg gtcacccggc tggatgcggc gggcgatgcg ctggccagcg
     3301 ccgcctacga tctgatcctg ctcgatctca tgctgccgga tggccggggc atcggctttc
     3361 tcaaggcgct gcgcgtccgg ggagacgtca cgcccgtcat catcctgaca gcgctcgatc
     3421 aggtatccga ccggatcgaa ggcctgaacg ccggcgccga cgactacctc gtcaagccct
     3481 tcgacctcgc cgagctctcg gcacggatcg gctcggtggc acggcgctac acgggcaacc
     3541 cgaacccgat catcacccac ggcgcgctcg acatcgacct cgccgcgcgc agcatccgcc
     3601 gggccggcag gcccatccag ctcaccgcgc gcgaatgggc gctgttcgag acgttcctcg
     3661 cacggcccgg ccagctcctg tcgaaggcgc agctcgagga gaagctctac gccttcgatg
     3721 ccgaggtcga gagcaacacg atcgaggtgc atgtcagccg gctgcgcaag aagctcgggg
     3781 ccggcgtgat cgagaccgag cgtggcctgg gctatcggct gggcacgcca tgaggttgcc
     3841 gcgcagcctc caggcacggc tcgggatctc gatcggcgct ctcctgacgc tgctctggat
     3901 cgccgccgcc tcggtgacgg cgatcgtgct gcggcacgag atgcaggagg tcttcgactc
     3961 gaccctgcag gaaacggcgc aacgcctcct gccgcttgcc gtcctcgaca tcgtcgaccg
     4021 cgaggcggat gacgacgccc agcggctcgc ccagatccgc gagcacgacg agcacttcac
     4081 ctatgtcgtg cgggacgccc tcggacaaat cctgctccag tcccacaccg ccgatcccct
     4141 gatctttccc acctatgacg gccccggctt cgcccagacg gcgacccaca gattctacag
     4201 cgaggaggcg ctgcggggca ccgtccggat caccgtggcg gagccaatgg agcaccgcgc
     4261 cgccatcgcg cgcgacatcc agatgggcct cggcctgccg gttctggtcg tgatccccgt
     4321 ggcgctcctg gcggtcgttc tcgccgtgcg ggcgaatctc gcaccgctcc ggcgctttcg
     4381 agaaaggctc gaagcgcgcc atgcccgcga tctgtcgccc gttccggcca gggacctgcc
     4441 ggcggagatc gccccgatcg cggccacgct gagcggcctt ctggaccggc tgaaggccgc
     4501 tttcgacgcc gagcgcaact ttgccgcgaa tgccgcccac gagcttcgga ccccgctcgc
     4561 cggtgcgatc gcccaggccc agcgcctgcg gtcggaaacc gccgacccgc aggccgaacg
     4621 acgggccggc gagatcgagg cgacgctcaa gcggctcacc cgcctcgccg agcgcctcat
     4681 gcagctcgcc cgggcggaag gcggccggct gcggctggat cgcagctcgg acctgcggct
     4741 cgtggcccgc gtggtcgtgg acgacatcga ccgaacgccg acccgcgacc gcgtcaccct
     4801 gacgctcgcg ggtcggccgg tgatgtcgga cctcgatccc gacgccttcg gcattctctg
     4861 ccgtaacctc gtcgacaacg cgctgcggca cggtgctgcg acgtctcccg tcgaggtgac
     4921 gttgagcgaa agcggcctgc tgaccgtcgc caacgagggg cccgtcgtgc cagccgagac
     4981 gctgagccgc ctgaccaccc gcttcgagcg cgccagccag cacacctccg gcagcggcct
     5041 cggcctcgcc atcgccgccg cgatcgccga ccgcatcggc agccggctcg tgctcagatc
     5101 accacggcca ggcaaatcgt ccgggttcga ggcggcggtc acccttccga catcggcctc
     5161 acccgctgca tcgatcgccg gcgccgacgc gaagggatag ctcccttgtg gacgagatgg
     5221 gacttcctct ttccccatcg gcgacttcgg atggctctcg tgtccccgcc tccctcgaga
     5281 caggcgccat cccgcccggt cgcgatgcgg ctttgagcgg acaggggatt gtagcagact
     5341 gggccgaggc gacggcattg gaggtccaag agccatgaac gacacgccag atgccgactg
     5401 ggacccgcgc tcgcatcagg tgcagtccga tcagcgcgcc gcgtatgacg ccatgcgcga
     5461 gacgtgcccg gtcgcctgga gcgactacat gcaatggtcg ctcttccgca acgccgacgt
     5521 cacccgcgcg gtgctggacc acgcgacctt cagcaacgcc gtctccaacc gcctgaccgt
     5581 gccgaacggc atggacccgc ccgagcacac cgcctggcgt cccatcgtcg agaagtattt
     5641 cacgagaagc cgcgtcgacg ccttcgagcc cgtctgccgc ggcatcgccg cggcgctgat
     5701 cgccgccgcg gcgggcccgg tcgagctgat gggggccatc gcccatccgt tcgcggttcg
     5761 cgtgcagtgc gccttcctcg gctggccgga cgagctcgcg gagccgctgc gccgctgggt
     5821 cgcgcgcaat cacgaggcca cgctcgcgcg cgaccacggt gccatgaccc gcatcgccgg
     5881 cgagttccag gacctgatcc gcgacaccat cgccgcgcgc atcgccgtcg gggcgacgcc
     5941 cgagaccgac gtcgccgtgg cgctcacgca ggagacgatc cacggccggc gcctgaacga
     6001 ggacgagatc accagcatcc tgcgcaactg gacggtgggc gagatcggca ccatcgccgc
     6061 gtcggtggga atcctggccg aatacctcgc cagcgatgtc ggcctgcagg gccggctgcg
     6121 tgccgaccct tcgctggccg gaccggccgc cgacgagatc ctgcgtctgc acggcccgct
     6181 cgtcaccaat cggcgcgtca ccacatgtcc ggtggagatc ggcggccgcc gcctcccggc
     6241 cggtgcccgc gtgaccgtca actgggtcgc ggccaaccgc gatccccggg tcttcgagga
     6301 tcccgacgtc ttccgcctgg atcgcgaccc gagcgacaac ctgctctatg gcgccggcat
     6361 ccacgtctgc cccggcgcac agctcgcccg catggagctg cgcgtgttcc tggaggagtt
     6421 gctggcaagg acctcagcga tcgctcccca ccggcccgcg acgccggcgg cctatcccgc
     6481 cagcggcttt cggacactgc atctgctgat ttcgtagggg gctcgcccag aagctcttct
     6541 tcaaggggcc gattgagggc gggatcgagg cccgagcgtc aggcggcccg gcgcctggtg
     6601 atgtggccgt tctcgatgta ccaggccgcc gtgcggcgga tgccttcctc gagcgggacc
     6661 ttgggcgcgt agccgagcac cgtccgggcg cgctcggtcg agaaggcgcg gtgcttgatg
     6721 aagaaggagg cgcgccgccg gtgcagcggc ggctcgatgc cgagcggctt gcagagcgcc
     6781 tcgcagagcg ccgagccggc cgagagcgtc cagaagggca ggcgcagctt cggcggcggc
     6841 acgtccagcg ctttggcgat ctcgcccacc cagtcgttca gcgagaccca gcggtcgccg
     6901 gcgatgatga aggtgccgcc caaggctgcc ggatgcgtgc cgcagagcag gatgccgtcg
     6961 atcaggtcgt cggcgtagac gaggtggtag aaggtctcgc ccgagccgaa catgacgaag
     7021 cggccgttgt tgaccgacct gaagagcttg aggaagcgca ggtcgcccgg cccgtacatg
     7081 ccggccgggc ggaagaccac gccgggcagg ccggccttga aggcttcctg cgccttcatc
     7141 tcgccctcga gcttggtcct ttggtagatg tcgcccggat tgaaggggct cgtctcggtg
     7201 cagggaaact cctgcacgtc gccatgcacg ccgatggtcg agcagtggat cgtccgctcg
     7261 acgccgtgct ttcgcgccgc ctcgagcacg ttgaccgtgc cgccgacatt gacgtcctcg
     7321 tagtagctgt cggggtggct cgccgtgcgg aagacggcgg cgatgtggta gaccttggca
     7381 cagccggcga cggccttgtc gacctccgcc gcatggcgga tgtcgccctg tgccagctcg
     7441 acgcccgccg ccttcaggtg gtcgaccctg gcgccgtcgc ggaccagggc gcggaccttg
     7501 tggccgtcct tgcgcaggcg cagcgccaga tggccgccgg cgaagccggt tgctccggtc
     7561 accaggatcg gcgcgtcgcc ggtcggcgtt tcaggctcca tggtcggcga tgtccttcgt
     7621 cgtttgcgga tgatgcccga aagccggacc gtggcgggcg agatcggcca ccgctgcggc
     7681 ccgctcggcc ggcgaccagc cgagctcctc ggccatgacg ccggcagcgg catccaggcg
     7741 cgccctgtct agccggcctc gggccacctc gtcgaggcgg cggacgagca catcgtccag
     7801 ccggcaggcc atctcgtgcc ggacgacgtg ccgcacgcgg gcgcggaaca cggtttcacc
     7861 ggtgagcggg ccttcgccgg catcgtcgag cacggcggcc cagccggcgc catggtcggc
     7921 gaccagccgg tcgatctccc gcccgccccc gggtgcccgc cttcgtgccg ccgccagcgc
     7981 cgccccggcc tcgaagccgc gggcaccggg caactgctcc tgccagctcc ggcagggcac
     8041 cgccgggcgg cccagcctcg ccagcgccag gtcgacgacc gattcggcga ccagccgggc
     8101 ggtcgtgtac ttgaccccct ggaggctcag cacgccctgg atcccgtccg ccggcccgtg
     8161 gtcgatcacg acgccgcgtc gcgtgcgcgc cacctcgctg ccgcgtgcct ccttcgccgc
     8221 cggggtgagg ccggcatagc agtagagcac gtcctcgcgc gtcagggcgg ccggcggcca
     8281 ggcgtcgttg atctcggcga ggaagtcgtc gagccagggc ccgtcgacga cgagctccgg
     8341 ggaatcgtcg gcatgcggca gatggctcgt gccgatgagg gtcagcccct gccagggctg
     8401 gatgaagaag agccggccgt cggcccgcgg caccagcgaa tcggagcgcc gccggctgct
     8461 cactgccagc gcgtggccgg tcgcgagcgg gcgccgcacg acgatgttca ggccgcgcgc
     8521 gaggccgccg aagtcgcgcc cggagcgggc cggcagacga tccaggagga gccccgcgtg
     8581 gggccccgcg gcattgatcg tgagcttcgc ccggatgtcg aattcctcgt cgccgagccg
     8641 gtcccgggcc ctcgcacccg tgacccgctc gggtgtgccg agaaagccca gggcctcgac
     8701 atggttggcg accacggcac cctccgccgc cgcgtccat
//
LOCUS       DAOTFI010000210         8677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9733_length_8677_cov_6.994201, whole genome shotgun sequence.
ACCESSION   DAOTFI010000210 DAOTFI010000000
VERSION     DAOTFI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9733_length_8677_cov_6.994201"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="P5558_18395"
     CDS             <1..512
                     /locus_tag="P5558_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRY26345.1"
                     /translation="NDVAVAARALLAEGAVRRVVVIDLDVHQGDGTAAILGSDPAVFT
                     LSAHCRTNFPARKERSTRDLALDPDMGDRAYLAAVEPLVEAALEAFRPDLVFYNAGVD
                     PHIDDRLGRLALTDQGLAARDAAVLEACRRRGLPLAVVVGGGYAEDVATVAARHALLH
                     EVAAELAPW"
     gene            complement(534..1766)
                     /locus_tag="P5558_18400"
     CDS             complement(534..1766)
                     /locus_tag="P5558_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY26346.1"
                     /translation="MAAGDGVIGSESPERAAYLVITGVSLCHFLNDVMQSLLAAIYPL
                     LRDEFSLDFWQIGLLTLVFQVTASLLQPLIGLYTDKRPVPQSLPVGMGSTLCGILLLA
                     TASSYPMLLLGASLIGVGSAVFHPEASRVARLASGGKYGAAQSFFQVGGNAGSATGPL
                     LAAFIVVPLGRPSIAWFALGALLGMAILSQVGAWYGRYRKANKGRVVPLIATGLSKRR
                     VTMAIAVLAVLVATKNFYTAGITSYYTFFLIERFGLTTQESQIMLFLFLGAMAVGVVI
                     GGPIGDRLGPLVVIWVSILGVLPFTLALPYVDLFWTGVLSVCIGVILASAFPAIIVYA
                     QELVPGRVGMIAGLFFGFAFGVGGLAAAALGFLADARGIEFVYRVCAFLPILGVLTIF
                     LPRRSRPGRRVASSAPAR"
     gene            2110..3147
                     /locus_tag="P5558_18405"
     CDS             2110..3147
                     /locus_tag="P5558_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY26347.1"
                     /translation="MVEQKIRNMEEFAAASGISRPTVSKYFNDPASVRRTTRERIEAA
                     LERFDYRPNIYAMNQNRRLTKTVGIVVPYLADPFFAEIARTIERLCIAAGYRTILLSS
                     HGEPALEIENLENLRLLRPAGVLLAPLGRASDRAAIERFCADVPAVLFDSRIDDVGEA
                     FIGSDNGQSMALIVDYLCRTGEPPCLFEMKTPTNPNARKRHAAYLAIMERLGHAPEVV
                     QVEGHDWEFEAIGYRGGLSAIERRRFATDTVLCSNDRLAIGLLAAAYEKGLRVGRGHG
                     CALRVAGHDDHPFARFTCPSLTTVSQDYAAIAARSVETLLALIDSDQTGAAFAPRVVL
                     FDGRLVMRQSA"
     gene            3256..4563
                     /locus_tag="P5558_18410"
     CDS             3256..4563
                     /locus_tag="P5558_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY26348.1"
                     /translation="MNLERSLYTASVLALIAAGSAQAQTTITIATVNNGDMIRMQGLT
                     EDFTAQTGHQVEWVILEENVLRQRVTQDIATSGGQFDIMTIGMYETPIWAAQDWLVPL
                     DDLPESYDADDILPAMRAGLSYEGTLYAAPFYGESSMVMYRTDLMEKAGLEMPEAPTW
                     DFIREAAAAMTDRDNEIYGICLRGKAGWGENMAFITTVANSFGARWFDEDWTPQLDSP
                     AWMEAVTFYNDLLANYGPPGASTNGFNENLSLFQQGKCGMWIDATVAASFVTNPNEST
                     VADKVGFALAPDKGLGKRANWLWAWALAIPAGTQKEEAAKDFINWATSKDYLALVAEK
                     EGWANVPPGSRTSLYENPEYLKAAPFAEMTLQSINAADPENPTVEPVPYVGIQYVAIP
                     EWAGIGTTAGQEFSAMVAGQQSPEQALEKAQTLVADEMEAAGY"
     gene            4704..5570
                     /locus_tag="P5558_18415"
     CDS             4704..5570
                     /locus_tag="P5558_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY26349.1"
                     /translation="MATQQSRSAARWMMAPAVVLLLGWMLVPLVMTLYFSFKQYLPLR
                     GGDLGWVGFDNYIRFVTSSAFWPSIQATLLIVGGVLVITVGLGILLALLLDQPMWGQG
                     IVRILVIAPFFVMPTVSALVWKNMFMDPVNGLLAHLWKFFGAQPIEWLSQVSMTSIII
                     IVSWQWLPFATLILLTAVQSLDTEQMEAAEMDGAPLLSRFWHLVLPHLSRAITVVILI
                     QTIFLLSIFAEIFVTTQGSFGTRTLTYLIFQRVLESQNVGLGSAGGVYAIILANIVAI
                     FLMRIVGKNLDA"
     gene            5583..6413
                     /locus_tag="P5558_18420"
     CDS             5583..6413
                     /locus_tag="P5558_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY26350.1"
                     /translation="MARAVTPKKKAINTAAAWAIGLLIFFPILWTILTSFKTEATAIA
                     DPPVFLFFDWTLENYQVVQDRSDYMRFLWNSVIIAGGSTLIGVMIAVPAAWSMAFVPS
                     KRTKDILLWMLSTKMLPAVGVLYPIYLIFISMGLLDTRTGLVFILMMMNLPIIVWMLY
                     TYFKEIPGEILEACRMDGAGLKEEILHILTPMAIPGIASTMLLNFILAWNEAFWTLNL
                     TAAQAAPLTAFIASYSSPEGLFFAKLSAASTMAIAPVLILGWFSQKQLVRGLTFGAVK
                     "
     gene            6456..7460
                     /locus_tag="P5558_18425"
     CDS             6456..7460
                     /locus_tag="P5558_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26351.1"
                     /translation="MGSIRLEQVNKTFGDVTVIPSVDLDIDDGEFVVFVGPSGCGKST
                     LLRLIAGLEDTTGGRILIDGKDATDTPPAKRGLAMVFQSYALYPHMSVRKNIAFPLRM
                     AKMDKAEQDRRIEQAAAVLNLTDYLDRRPGQLSGGQRQRVAIGRAIVREPAAFLFDEP
                     LSNLDAALRVGMRLEISELHERLKTTMVYVTHDQVEAMTMADKIVVLRAGNIEQVGSP
                     LELYHTPRNLFVAGFIGSPRMNFIEGPEAARYNAHTIGIRPEHIAVSADEGTWSGVVS
                     VSEHLGSDTFFHVHDSGLAESITVRAGGEVGFRHGDRIFMTPREDVLHRFSADGLRLA
                     "
     gene            7500..>8677
                     /locus_tag="P5558_18430"
     CDS             7500..>8677
                     /locus_tag="P5558_18430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HRY26352.1"
                     /translation="MKLSNATLGRLPDSILRPRYDRRALRPGIVHIGVGNFHRAHQSW
                     YLHRLMQQGQALDWAILGAGVRPYDARMRQKLLDQDCLTTLIELDPSGRSAEVVGSMI
                     DYLPIEDGNGALVAAMADPAIRIVGLTVTEGGYYVDPVSRGFDAAHPDIIHDAANPDT
                     PRTAFGAMIAALQHRRDAGHGPFTALSCDNLQGNGAILRQTLVSLARLSNPDLADWID
                     ERCTFPSSMVDCIVPATGPDELALARSLGIDDAAPVTHENFRQWVIEDVFCAGRPDWD
                     RVGATFTDRVHDYETMKIRILNAGHQVLANAGEILAVPTIAACMAHPLIGPFFRKVEL
                     EEIAPHVHAVPGMTPEEYVELIDRRFSNPAIQDTTRRVAFDGSARHTGFVLPILRDGL
                     A"
BASE COUNT         1460 a         3259 c         2642 g         1316 t
ORIGIN      
        1 tcaacgatgt cgccgtggcc gcccgggcgc tgctcgccga gggcgccgtg cggcgggtcg
       61 tggtcatcga tctcgacgtc caccagggcg acggcacggc ggcgatcctg gggtcggatc
      121 cggccgtctt caccctgtcg gcgcattgcc ggaccaactt cccggcgcgc aaggaacgca
      181 gcacccgcga tctcgccctc gaccccgaca tgggcgaccg cgcctacctc gccgccgtcg
      241 agcccctggt cgaggcggcg ctcgaggcgt ttcgccccga cctcgtcttc tacaatgccg
      301 gcgtcgaccc gcacatcgac gaccgcctcg gccgcctcgc cctcaccgac cagggcctcg
      361 ccgcccgcga cgccgccgtc ctcgaggcct gccgccgccg gggcctgccg ctcgcggtcg
      421 tcgtcggcgg cggctatgcg gaggacgtcg ccaccgtcgc cgcccgccac gccctcctgc
      481 acgaggtcgc ggccgagctc gccccctggt gaggccgctc ggcgcgcccc gggtcagcgg
      541 gccggggcgc tgctcgcgac ccgccgcccc ggccggctgc gccgcggcag gaagatggtg
      601 agcacgccga ggatcggcag gaacgcgcag acccggtaga cgaactcgat gccccgggcg
      661 tcggcgagaa agccgagcgc cgccgccgcc agcccgccca cgccgaaggc gaagccgaag
      721 aagagcccgg cgatcatgcc gacccgcccc ggcaccagct cctgcgcata gacgatgatg
      781 gccgggaagg ccgaggccag gatgacgccg atgcagaccg agagcacccc ggtccagaag
      841 aggtccacgt agggcaaggc gagggtgaag ggcagaacgc ccaggatcga gacccagatg
      901 accacgagcg ggccgagtcg gtcgccgatc ggcccgccga tcacgacccc gaccgccatg
      961 gcgccgagga agaggaagag catgatctgg gactcttgcg tcgtcaggcc gaatcgctcg
     1021 atgaggaaga acgtgtagta gctggtgatg ccggccgtgt agaaattctt ggtcgcgacc
     1081 agcaccgcca gcacggcgat cgccatggtc acccgccgct tggacaggcc cgtggcgatt
     1141 aagggcacca cccggccctt gttggccttg cgatagcgcc cgtaccaggc gcccacctgg
     1201 ctcaggatcg ccatgccgag cagggccccg agcgcgaacc aggcgatgct cggccggccc
     1261 agcggcacca cgatgaaggc ggcgagcagg gggcccgtcg cactgcccgc attgccgccg
     1321 acctggaaga acgactgcgc ggcaccgtac ttgccgcccg aggccagccg cgccacccgc
     1381 gacgcctcgg gatgaaagac cgccgagccc acgccgatca gcgaagcccc cagcagcagc
     1441 atcggatagc tcgaggcggt ggccagaagg agaatgccgc agagcgtcga gcccatgccg
     1501 accggcaggg actgcggcac cggccgcttg tccgtgtaga gcccgatcaa gggctgcagc
     1561 agcgacgccg tcacctggaa gaccagcgtc aagagcccga tctgccagaa atcgaggctg
     1621 aactcgtcac gcagcagggg gtagatcgcc gccagcagcg actgcatcac atcgttgagg
     1681 aaatggcaga ggctgacgcc tgtgataacc agataagccg cccgctcggg gctctcgctc
     1741 ccgatcacac cgtcacccgc cgccatctct tcagccatcc ccgccaatgg acccgaagcg
     1801 gcgacggaac gacctccgcc gaccgaatgt caagggtggc gtggggctgg ccgccgcctt
     1861 gcgcctgcgc tcgaaggatg gcccgtcgcg ccgggccgcg gtcgctcgtt ccgccgcacg
     1921 aacgcgacga aggtgacgga acgactcgcc agcgccggcc acgcccatga tccgctcgcc
     1981 atgctccgcc gtgtcgagcg aaagccttga cggtgcaatt tcgccgcgcc tagtgtgcct
     2041 cgagaaattt gcgcgcgtaa tatgtttttt gtgcgcgaga cgtaatctct cagctttccg
     2101 ggcgagggca tggtcgagca gaagatcagg aacatggagg aattcgccgc tgccagcggc
     2161 atctcccggc cgaccgtctc gaaatacttc aacgacccgg cgagcgtgcg gcgcaccacg
     2221 cgcgagcgca tcgaggcggc gctcgagcgc ttcgactacc gcccgaacat ctacgcgatg
     2281 aaccagaacc gccgcctgac gaagacggtc ggcatcgtcg tcccctacct cgccgacccg
     2341 ttcttcgccg agatcgcccg caccatcgag cgcctgtgca tcgccgccgg ctatcgcacc
     2401 atcctgctca gctcgcacgg cgagccggcg ctggagatcg agaacctcga gaacctccgc
     2461 ttgctccggc cggcaggcgt cctcctcgcc cccttgggcc gcgcttccga ccgggccgcg
     2521 atcgagcgct tctgcgccga cgtgccggcc gtcctgttcg acagccgcat cgacgacgtg
     2581 ggcgaggcct tcatcggctc ggacaacggg cagagcatgg cgctcatcgt cgactatctc
     2641 tgccggaccg gcgagccgcc ctgcctcttc gagatgaaga cccccaccaa cccgaacgcc
     2701 aggaagcgcc atgccgccta cctggcgatc atggagcggc tgggccacgc gccggaggtc
     2761 gtgcaggtcg agggccacga ctgggagttc gaggcgatcg gctatcgggg cggcctgagc
     2821 gcgatcgagc ggcgccggtt cgccaccgac accgtgctct gcagcaacga ccgcctcgcc
     2881 atcggcctgc tcgccgccgc ctacgagaag gggctcaggg tcggtcgcgg gcacggctgc
     2941 gcgctcaggg tcgccggcca cgacgaccat cccttcgcgc gcttcacctg cccctcgctc
     3001 acgaccgtgt cgcaggacta cgccgccatc gcggcgcgca gcgtcgagac cctgctggcc
     3061 ctcatcgatt ccgaccagac cggcgctgct ttcgccccca gggtcgtgct gttcgacggc
     3121 aggctggtca tgcgccagtc ggcctgaaac cggactcgtg acaataagtt ggcgcgcgtc
     3181 aaatttccga ttgacacacg gcggcggcct gtcttagctt ccattcgcca aacgcacata
     3241 aaagggagcg gccagatgaa cctcgagcgt tcactctaca cggcatcggt cttggcgctg
     3301 atcgcggccg gcagcgcgca ggcgcagacc accatcacca tcgccacggt caacaacggc
     3361 gacatgatcc gcatgcaggg gctgaccgag gacttcacgg cccagaccgg gcatcaggtc
     3421 gaatgggtga tcctggagga gaacgtcctg cggcagcgcg tgacgcagga catcgcgacc
     3481 agcggcggcc agttcgacat catgacgatc ggcatgtacg agacgccgat ctgggccgcg
     3541 caggactggc tggtcccgct cgacgatctg ccggaaagct acgacgccga cgacatcctg
     3601 cctgccatgc gcgcgggcct gtcctacgaa ggcacgctct atgccgcacc gttctacggc
     3661 gagagctcga tggtcatgta ccgcaccgac ctgatggaga aggcgggcct ggagatgccc
     3721 gaggccccga cctgggactt catccgggaa gccgcggcgg cgatgaccga cagggacaac
     3781 gagatctacg gcatctgcct gcgcggcaag gccggctggg gcgagaacat ggcgttcatc
     3841 accaccgtcg ccaactcgtt cggcgcgcgc tggttcgacg aggactggac cccgcagctc
     3901 gacagccccg cgtggatgga ggcggtgacc ttctacaacg acctgctcgc caattacggc
     3961 ccgcccggcg cctcgaccaa cggcttcaac gagaacctgt cgctcttcca gcagggcaag
     4021 tgcggcatgt ggatcgacgc caccgtggcc gcctccttcg tgaccaaccc gaacgaatcg
     4081 acggtcgccg acaaggtcgg cttcgccctg gcacccgaca agggcctggg caagcgggcc
     4141 aactggctct gggcctgggc gctggccatt ccggcgggca cccagaagga agaggcggcc
     4201 aaggacttca tcaactgggc cacctcgaag gattatctgg cgctcgtcgc ggagaaggaa
     4261 ggctgggcca acgtgccgcc cggcagccgc acctcgctct acgagaaccc cgagtacctg
     4321 aaggcggcgc ccttcgccga gatgacgctg cagtcgatca acgccgcgga tccggagaac
     4381 ccgaccgtcg agccggtgcc ctatgtcggc atccagtatg tcgcgatccc cgaatgggcc
     4441 ggcatcggca cgaccgccgg ccaggagttc tcggccatgg tcgcggggca gcagtccccc
     4501 gagcaggccc tggagaaggc gcagaccctc gtcgccgacg agatggaagc cgccggctac
     4561 tgatcgggct ccctcccggc ccggaggggg cggccacctg ccgtcccctc cggccccgga
     4621 accccgcagg cctgcgccca tgcggccttc gagggcagca acgttcctga caggatacgg
     4681 tcagacaggg aaggagccgc ggtatggcca cgcaacaatc ccgttctgcc gcccggtgga
     4741 tgatggcgcc cgccgtggtc ctcctcctcg gctggatgct cgtccccctg gtcatgaccc
     4801 tctacttctc gttcaagcag tatctgccac tgcgcggcgg tgatctcggc tgggtcggct
     4861 tcgacaacta catccgcttc gtcacctcga gcgccttctg gccgtcgatc caggcgacgc
     4921 tgctgatcgt cggcggcgtg ctggtcatca cggtcgggct cggcatcctc ctggccctcc
     4981 tgctcgacca gccgatgtgg ggacagggca tcgtccgcat cctcgtcatc gcccccttct
     5041 tcgtcatgcc gaccgtctcc gccctggtct ggaagaacat gttcatggat cccgtgaacg
     5101 gtcttctggc gcatctctgg aaattcttcg gcgcgcagcc gatcgaatgg ctgagccagg
     5161 tctcgatgac gtccatcatc atcatcgtct cctggcaatg gctgccgttc gcgacgctca
     5221 tcctgctcac cgccgtgcag tcgctcgata ccgagcagat ggaggcggcc gagatggacg
     5281 gcgccccgct gctcagccgc ttctggcacc tggtcctgcc gcacctctcg cgggcgatca
     5341 ccgtggtgat cctgatccag accatcttcc tgctctcgat cttcgccgag atcttcgtca
     5401 ccacccaggg ctcgttcggc acccgcacgc tgacctacct gatcttccag cgcgtgctgg
     5461 agagccagaa tgtcgggctc ggctcggccg gcggcgtcta tgcgatcatc ctggccaaca
     5521 tcgttgccat cttcctgatg cgcatcgtcg gcaagaacct ggacgcgtga ggagacagac
     5581 ccatggcccg cgccgtcacg ccgaagaaga aagcgatcaa caccgccgcc gcctgggcga
     5641 tcggcctcct gatcttcttc ccgatcctct ggacgatcct gaccagcttc aagacggaag
     5701 ccacggcgat cgccgatccc cccgtcttcc tcttcttcga ctggaccctg gagaactacc
     5761 aggtcgtcca ggaccgctcc gactacatgc ggttcttgtg gaactccgtg atcatcgccg
     5821 gcggctcgac cctgatcggc gtcatgatcg ccgtccccgc cgcctggtcg atggccttcg
     5881 tgccctcgaa gcggaccaag gacatcctgc tctggatgct ctccaccaag atgctgccgg
     5941 cggtcggcgt gctctacccg atctacctga tcttcatctc catggggctc ctcgacaccc
     6001 gcaccggcct cgtcttcatc ctgatgatga tgaacctgcc gatcatcgtc tggatgctct
     6061 acacctactt caaggagatc ccgggcgaga tcctcgaggc ctgtcgcatg gacggcgccg
     6121 gcctgaagga ggagatcctc cacatcctga caccgatggc catcccgggc atcgcgtcca
     6181 ccatgctcct gaacttcatc ctcgcctgga acgaggcgtt ctggacgctg aacctgacgg
     6241 ccgcccaggc ggccccgctc accgccttca tcgccagcta ttccagcccg gaaggcctct
     6301 tcttcgccaa gctttccgcc gcctcgacca tggccatcgc gccggtgctc atcctcggct
     6361 ggttcagcca gaagcagctg gtccgcggcc tgacgttcgg cgccgtcaaa tgaccggcca
     6421 ccgccagtcg aaccagcagg atcgggagaa cgcccgtggg tagcatcagg ttggagcagg
     6481 tcaacaagac cttcggcgac gtcaccgtca tccccagcgt cgacctcgac atcgacgacg
     6541 gcgagttcgt cgtcttcgtc ggcccctccg gctgcggcaa gtccaccctg ctccgcctga
     6601 tcgccggcct cgaggacacc accggcgggc gcatcctgat cgacggcaag gacgccaccg
     6661 acaccccgcc cgccaagcgc ggcctcgcca tggtcttcca gtcctacgcc ctctacccgc
     6721 acatgagcgt gcgcaagaac atcgccttcc cgctgcgcat ggcgaagatg gacaaggccg
     6781 agcaggaccg ccggatcgag caggcggccg ccgtcctcaa cctcaccgac tacctcgacc
     6841 gccggcccgg ccagctctcg ggcggccagc gccagcgcgt cgccatcggc cgcgccatcg
     6901 tccgcgagcc cgccgccttc ctcttcgacg agccgctctc caacctcgac gccgcccttc
     6961 gcgtcggcat gcggctcgag atcagcgagc tgcacgagcg gctgaagacc accatggtct
     7021 acgtcaccca cgaccaggtc gaggccatga ccatggccga caagatcgtg gtcctgcggg
     7081 ccggcaatat cgagcaggtg ggctcgccgc tcgagctcta ccacaccccg cgcaacctct
     7141 tcgtcgccgg cttcatcggc tcgcccagga tgaacttcat cgaggggccg gaggccgccc
     7201 gctacaatgc ccacacgatc ggcatccgcc ccgagcacat cgcggtctcg gccgacgagg
     7261 ggacctggtc cggggtcgtc agcgtctccg agcacctggg ctccgacacc ttcttccacg
     7321 tccacgattc cggcctcgcc gaaagcatca cggtgcgcgc cggcggcgag gtgggcttcc
     7381 gccacggcga ccgcattttc atgacccccc gtgaggacgt cctccaccgc ttctcggccg
     7441 acggcctcag gctcgcctga cccggccata tcgatcgaga aaaaaaaatg gacaggccga
     7501 tgaagctgtc gaacgccacc ctgggccggc tccccgattc catcctgcgg ccgcgctacg
     7561 accgaagggc gcttcgaccc ggcatcgtcc atatcggcgt cggcaacttc caccgcgccc
     7621 accagtcctg gtacctccac cgcctgatgc agcagggcca ggccctggac tgggcgatcc
     7681 tcggcgccgg cgtgcgcccc tatgacgccc ggatgcgcca gaagctcctc gaccaggact
     7741 gcctgaccac gctgatcgag ctggacccct ccggccggtc cgccgaggtc gtgggctcca
     7801 tgatcgacta cctgccgatc gaggacggca acggcgccct cgtcgccgcc atggccgacc
     7861 ccgccatccg catcgtcggc ctcaccgtca ccgagggcgg ctactatgtc gacccggtca
     7921 gccgcggctt cgatgccgcc catcccgaca tcatccacga cgccgccaac cccgacaccc
     7981 cccgcaccgc cttcggcgcg atgatcgcag ctctgcagca ccgccgcgac gccggccacg
     8041 ggcccttcac cgccctgagc tgcgacaacc tccagggcaa cggcgccatc ctccgccaga
     8101 ccctcgtctc gctcgcccgg ctctccaacc ccgacctcgc cgactggatc gacgagcgct
     8161 gcaccttccc cagctccatg gtcgactgca tcgtcccggc gaccggcccg gacgagctcg
     8221 ccctcgcccg ctcgctcggc atcgacgacg ccgcacccgt gacccacgag aacttccgcc
     8281 agtgggtgat cgaggacgtt ttctgcgccg gccggcccga ctgggaccgc gtcggcgcca
     8341 ccttcaccga tcgcgtccac gactacgaga ccatgaagat ccgcatcctc aatgccggcc
     8401 accaggtcct cgccaatgcc ggcgagatcc tggccgtgcc caccatcgcc gcctgcatgg
     8461 cccaccccct catcggcccc ttcttcagga aggtcgagct cgaggagatc gccccccacg
     8521 tccacgccgt ccccggcatg acccccgagg aatatgtcga gctcatcgac cgccgcttct
     8581 ccaaccccgc catccaggac accacccgcc gcgtcgcctt cgacggctcc gcccgccaca
     8641 ccggcttcgt cctccccatc ctccgcgacg gcctggc
//
LOCUS       DAOTFI010000211         8666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9756_length_8666_cov_6.974451, whole genome shotgun sequence.
ACCESSION   DAOTFI010000211 DAOTFI010000000
VERSION     DAOTFI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9756_length_8666_cov_6.974451"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="P5558_18435"
     CDS             complement(<1..614)
                     /locus_tag="P5558_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY26353.1"
                     /translation="MDYETIFSAAIGKVRDEGRYRTFADLARRVGGFPAADHRCPDRG
                     RREVTIWCSNDYLGMGQHPVVLEAAERALRDYGAGAGGTRNISGTTHLHVLLEQELAA
                     LHGKPAALVMTSGYVANEAAISTLATLLPDCVIFSDELNHASMIAGVRASRCTKRVFR
                     HNDLEHLEELLAAEPAGRAKLIVFESVYSMDGDFGPINAVCDLA"
     gene            complement(676..1626)
                     /locus_tag="P5558_18440"
     CDS             complement(676..1626)
                     /locus_tag="P5558_18440"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRY26354.1"
                     /translation="MSSVASGVRTPERVEWPTVGLALVMVGGLVALTWWHAALPWFVL
                     LPLGAYLGAWYSSLQHEIVHGHPTPSPLVNTAIASLPVLLWLPFGIYRATHLAHHADE
                     HLTDPLDDPESYYVTAATWRRLPGWRRRLLLARNSLLGRLTLGSVQVVLAFLVDQARA
                     LVAHAPGVRRAWLAHAAGLILWLGWLALVQMPLWQYVLLVAWPATSLMLVRSFLEHQA
                     RPAVDQRTVIVEAGPLMRLLYLNNNLHLVHHAKPGLPWYLLPEAYARDRAGWLRRNGG
                     YVFEGGYAEILRRYALRAKEPPVHPFADDRARETAPAAAY"
     gene            complement(1725..2597)
                     /locus_tag="P5558_18445"
     CDS             complement(1725..2597)
                     /locus_tag="P5558_18445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26355.1"
                     /translation="MEQIVADDGRAPLKGIALMLVTMLTFASQDAITKLLAQDLHVAQ
                     IVMVRYWLFAAFATVLIARQPGGLRRAVQARRPWLQVIRAAIFVVEVGVFAWVVAHMP
                     LSTAQALFAGCPLMVTALSPFVLGEKVGRLRWIAVIAGFVGVLIILRPGGEDFYPVGV
                     VAVIGTAMFAVYSLMTRLLAKEDRFETSYGYVAWVGCLMASAVGPFVWQSPSPDQIIG
                     LLVIASTGVFGHMILMKAYEIAPASVLQPFSYTQLVWGIFIGIMVFGEYPDGYMLLGA
                     GIVVVAGIIAMRAR"
     gene            3225..3854
                     /locus_tag="P5558_18450"
     CDS             3225..3854
                     /locus_tag="P5558_18450"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRY26356.1"
                     /translation="MTEPSPLSSRRLTWRPAWLATALLAASPYAAAVAVAEPASWVID
                     PGNSSIGFAANHIGYKDVLGLFLEERGSFTFDEATLELSDLVFEVEAASVFTDNPARN
                     EHLLSDDFLAAESHPLIRFVMTEALPATATTGTIKGDLTVRGVTRPAEVDVTLNRIAP
                     YPWGSNYVMGVSAEAVVRRSAFGSTYALEGGLVADEIFLAFEIEAIRQD"
     gene            3860..4618
                     /locus_tag="P5558_18455"
     CDS             3860..4618
                     /locus_tag="P5558_18455"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRY26357.1"
                     /translation="MHHALTRLRGLGRSLAMYYWPPGSTARLARFYGQFVRPGDLCFD
                     IGAHVGNRSLAFARLGARVVAVEPQPDFARFLRHLLARRRGVVLEEVAIAAAPGRVEL
                     HLSPATPTVTTASAAFIDGVAAIPSFAGVRWENRVEVPATTLDLLIERHGLPDFLKLD
                     IEGLEEAALAGLSRAPKLLSFEFLAGRMASAAHCLDRLEAIGGWTFNLSIGESLELEW
                     PDFVPRERLDAWLAARAGEDFSGDIYARRLDVAS"
     gene            4615..5361
                     /locus_tag="P5558_18460"
     CDS             4615..5361
                     /locus_tag="P5558_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRY26358.1"
                     /translation="MSVEPGTYWPTLLQERRLPAAAAADPLACAYLDFARPERVVLGH
                     LGQSLDGYVATLAGHSANMTGAANIRHLHRLRALADAIVVGAGTVLNDDPRLTTRLVA
                     GPSPVRVVLDNRRRLGADYRLFREGPPTLVLCAADAADGATLGRAEIVPVETADGSVA
                     PAAVLEALGTRGLKRVFVEGGGVTVSRFLFGGCLDILEICVSPLVLGSGRRGLAGPAP
                     ERTDEALRLEGCRAIPMGADMLFHQKLRPG"
     gene            5414..5911
                     /locus_tag="P5558_18465"
     CDS             5414..5911
                     /locus_tag="P5558_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY26359.1"
                     /translation="MRRTFVRAAQPDEAAALSALALRSKAHWGYRPAFMAMCVAELTV
                     TPELIDEGLALVAERSGRIAGFIVCTPVDAASVELDMIFVEPEAIGQGIGRLLVDAAF
                     AGLARRGVRRVEVASDPHAEPFYRSCGFEPVGTVPSGSIPGRFLPRLCFALDERYCVA
                     PKEAR"
     gene            5911..6681
                     /locus_tag="P5558_18470"
     CDS             5911..6681
                     /locus_tag="P5558_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY26360.1"
                     /translation="MYRDKYDLTGRVAVVTGGGRGIGFESARALAECGAHVVIAELDA
                     ATGPQAAAELERLGCSAEFVPLDVTQSAAVTATADALQERHGRIDILVANAGIAKGTP
                     GEDMSDEEWLLVLDINLNGVFWCNRAFGRHMLARGRGSIVNIGSMSGLISNKPQPQSA
                     YNASKSGVHMLTRSLGGEWATRGVRVNAVAPTYIATEMTRMGRENPEWLPTWLEMTPM
                     RRLGEPHEIASAVLFLASDASSLMTGSVVVVDGGYTIW"
     gene            6693..6986
                     /locus_tag="P5558_18475"
     CDS             6693..6986
                     /locus_tag="P5558_18475"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRY26361.1"
                     /translation="MPAYVIATIEVTDPEAYEAYKTAAAAAIAKHGGRYLARGGRSHV
                     AEGAFPGSRFVLLEFADYATAQGFVASADYQAAKALRAGAAEMNMVIIEGYVP"
     gene            6983..7768
                     /locus_tag="P5558_18480"
     CDS             6983..7768
                     /locus_tag="P5558_18480"
                     /EC_number="1.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008854842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HRY26362.1"
                     /translation="MKLKDRVAVITGGARGIGRAIAERYADEGARVVVADILEAEAKA
                     TAQAIGRGAFGLKVDVTDQASIDAMVKAVVDEAGRIDILVNNAAIFNMAPVLEVTRED
                     WDRNFDINTKGLFFTLQAVARRMVEQAAADPAWRGKIINMASQAGRRGESLVSAYCAS
                     KAAVISITQSTAKGLIPHRINVNGIAPGVVDTPMWDEVDRLFAKYENLPIGEKKRQVG
                     EAVPFGRMGVPEDHAGAAVFLASSDADYVLAQTLNVDGGNWMS"
     gene            7786..>8666
                     /locus_tag="P5558_18485"
     CDS             7786..>8666
                     /locus_tag="P5558_18485"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRY26363.1"
                     /translation="MPFTLGVNTNAFVNRIVEPEELVRTVVEKIGLRRIQLTPEILSP
                     DWPASVIAAQVRRWNAACEKHGARITTTFTGNYTRLNSLCHPDPDVRAHWRAWLGRLI
                     DITADLGGAATGSQIGILTYADDRDPARRAARFAEARDHWHALAGHAKERGLACLMWE
                     PMSISREFGQTIEACRRDHAALNEGSPLPILINADIDHGDVTSSDPADTDPYAWAEAL
                     AQVSPVVHIKQSSMDKGGHRPFTAAYNAKGRVVPERLIQALERGGGRDNELVFEFTFR
                     EREPTDSEVVAALKESV"
BASE COUNT         1368 a         3085 c         2968 g         1245 t
ORIGIN      
        1 tcggcgaggt cgcagaccgc gttgatcggg ccgaaatcgc cgtccatcga gtagacgctc
       61 tcgaagacga tcagcttggc ccgcccggcc ggctccgccg cgagcagctc ttccagatgc
      121 tcgaggtcat tgtggcggaa gacccgcttc gtgcagcggc tcgccctgac accggcgatc
      181 atcgaggcgt ggttgagctc gtccgagaag atcacgcagt cgggcaggag ggtggccaaa
      241 gtcgagatcg ccgcctcgtt ggcgacgtag ccagacgtca tgacgagggc cgccggcttg
      301 ccgtgcaggg cggcgagctc ctgctcgagc agcacgtgga gatgcgtcgt gcccgagatg
      361 ttgcgggtgc ccccggcgcc ggcaccgtag tcccgaagcg cccgctcggc ggcctcgagc
      421 accactggat gctggcccat gcccagatag tcgttcgagc accagatcgt cacctcgcgc
      481 cggcccctat cggggcagcg atggtcggcg gcaggaaacc cgccgacacg gcgggcgaga
      541 tcggcgaagg tccgataccg gccttcgtcg cgcaccttgc cgatggcggc agaaaagatc
      601 gtctcgtagt ccatgttcac gatccccgac gtcttccggc caccctacat cctccggcac
      661 ttccgggaaa accggtcagt aggccgcagc cggggcggtt tctcgcgccc ggtcgtcggc
      721 gaaggggtgc accggcggct ccttcgcccg gagcgcgtag cggcggagga tctcggcgta
      781 gccgccctcg aacacatagc cgccattgcg ccgcagccag cccgcccggt ccctcgcata
      841 ggcctcgggc agaaggtacc agggcaggcc gggcttggca tgatgcacga gatgcaggtt
      901 gttgttgagg tagagcagcc gcatcagcgg cccggcctcg acgatcacgg tccgctggtc
      961 gaccgccgga cgcgcctggt gctcgaggaa ggagcgcacc agcatgagcg aggtggcggg
     1021 ccaggcgacg aggagcacgt actgccagag cggcatctgg acgagcgcca gccagccgag
     1081 ccagaggatg agcccggccg cgtgcgcgag ccaggcgcgc cgcacgcccg gcgcatgggc
     1141 gacgagggcc cgggcctggt ccaccaggaa ggcgaggacg acctgcacgc tgccgagcgt
     1201 cagccggccg agaagcgagt tgcgcgccag cagcaggcgc cggcgccaac ccggcagccg
     1261 gcgccaggtg gcggcggtca cgtagtagct ctcggggtcg tcgagcgggt cggtcagatg
     1321 ctcgtccgca tggtgcgcca gatgcgtcgc ccggtagatg ccgaacggca gccagagcag
     1381 caccggcagg ctggcgatgg ccgtgttcac cagggggctc ggggtcggat ggccgtggac
     1441 gatctcgtgc tgcagcgagc tgtaccaggc gccgagatag gcgccgagcg gcaggagcac
     1501 gaaccacggc agggcggcat gccaccaggt cagcgccacg aggccgccga ccatcacgag
     1561 cgccaggccg accgtcggcc actcgaccct ctcgggtgtg cgcacgcccg acgccacgct
     1621 gctcatctcg ttctggcccc accgcccgga tcggggcatt cgagccccgc atcatcaagg
     1681 cggtcattcg ccgggtcctg tcaatatccc gtgagcggca ggactcagcg ggcgcgcatg
     1741 gcgatgatgc cggcgaccac gacgatgccg gcgccgagca gcatgtaccc gtccggatac
     1801 tcgccgaaga ccatgatgcc gatgaagatg ccccagacga gctgcgtgta gctgaacggc
     1861 tgcagcacgc tcgccggcgc gatctcgtag gccttcatca ggatcatgtg gccgaagacg
     1921 ccggtcgagg cgatcaccag gagcccgatg atctggtcgg gtgacgggct ctgccagacg
     1981 aaggggccga ccgccgaggc catcaggcag ccgacccagg cgacgtagcc gtaggaggtc
     2041 tcgaagcggt cctccttggc gaggagccgg gtcatgaggc tgtagaccgc gaacatggcg
     2101 gtgccgatga cggcgacgac gccgaccggg tagaagtcct cgccgccggg cctgaggatg
     2161 atcaggacgc cgacgaagcc ggcgatgacg gcgatccagc gcagccgccc caccttctcg
     2221 ccgaggacga agggcgagag ggcggtgacc atgagcgggc agccggcgaa gagcgcctgc
     2281 gccgtgctga gcggcatgtg ggccaccacc caggcgaaga cgccgacctc gacgacgaag
     2341 atcgcggccc ggatgacctg cagccagggg cgacgggcct gcaccgcccg ccgcaggccg
     2401 cccggctgcc gggcgatcag caccgtggcg aaggcggcga acagccagta gcggaccatg
     2461 acgatctgcg cgacatgcag atcctgggcg agcagcttgg tgatcgcgtc ctgcgaggcg
     2521 aaggtgagca tcgtcaccag catcaaggcg atgcccttca ggggcgccct gccgtcatcg
     2581 gccacgatct gctccatcgg tgtggccgga caggctctga acggccggcg tttcggaggg
     2641 ctctctagca ggctcccgag ggagccgcga caggctcccg cgagtcggga gagtggattg
     2701 atatgggctt cagggaatgc gcgtaatgcg cgagttcgcg ccgcgcgccc ggcgcgaacg
     2761 tccatctgcc tgatgtgaaa gcggaatgac cgttcatcga caaagaatcc acaccccgat
     2821 gaacagccct atccccgccg atgtggataa tcgtcaacgc ggtctcgcgg ccttccctcg
     2881 atgccccggg tgcaacggtt gaattgcccc cggcaggctt tccattgcct cgggcggccc
     2941 gaaacccgtc gtcaactacc tgagaacaaa gggttcttcc cgggatatgg cgcaaagggg
     3001 agcgccgcgg ggatgccgct ccctttgagc cggccgagct ctggagggaa tccgggtatc
     3061 cggcgcgatg cggggcttgg gacattttcg cacagtctgt ggataacgtg gatgatttca
     3121 cgggcccgat ggtgtcgacc ggcgccgtgg tcggggcgag gggtgttccg gagccccgac
     3181 agagctatag tttagcggca aacgtgctcc agccggatga tcccatgacc gagccgtcac
     3241 ccctgtccag ccggcgcttg acatggcgcc ccgcgtggct cgcgaccgcg cttctcgccg
     3301 cctcgcccta cgctgctgcc gtggcggtcg ccgagccggc aagctgggtg atcgacccgg
     3361 gcaattcgtc gatcggcttc gctgccaacc acatcggcta caaggacgtg ctcggcctct
     3421 ttctcgagga gcgcggcagc ttcaccttcg acgaggcgac gctggagctc tccgacctcg
     3481 tcttcgaggt ggaggccgcc agcgtcttca ccgacaaccc ggcccgcaac gagcatctcc
     3541 tgagcgacga cttccttgcc gccgaaagcc acccgctgat ccgcttcgtc atgaccgagg
     3601 cgctgcccgc gaccgccacc accgggacca tcaagggcga tctcacggtc cgcggcgtca
     3661 cgcggccggc cgaggtcgac gtcaccctga accgcatcgc cccctatccc tggggcagca
     3721 actacgtcat gggcgtcagc gccgaggccg tcgtccggcg cagcgcgttc ggcagcacct
     3781 acgcgctcga gggcgggctc gtcgccgacg agatcttcct cgccttcgag atcgaggcga
     3841 tccgccagga ctgaccacct tgcaccatgc cctgacacgg ctgcggggcc tcggccgctc
     3901 gctcgccatg tactactggc cgcccggcag caccgcccgg ctcgcccggt tctatggcca
     3961 gttcgtgcgg ccgggcgatc tctgcttcga catcggcgcc catgtcggca atcgcagcct
     4021 cgccttcgcc cgcctgggtg cccgggtggt ggcggtcgag ccgcagcccg atttcgcccg
     4081 gttccttcgt catctgctgg cgcgacgccg gggagtggtg ctggaggagg tggccatcgc
     4141 cgctgcaccc ggcagggtcg agctgcatct gagcccggcg acgccgacgg tcacgacggc
     4201 ctcggccgcc ttcatcgacg gggtcgccgc catcccgtcc ttcgccgggg tacgctggga
     4261 gaaccgggtc gaggtgccgg ccacgaccct cgacctgctg atcgagcgcc acggcctccc
     4321 cgacttcctc aagctcgaca tcgagggact ggaggaggcg gcgctcgccg ggctgagccg
     4381 ggcaccaaag ctcctctcct tcgagttcct ggccggaagg atggccagcg ccgcgcactg
     4441 cctcgaccgg ctggaggcga tcggcggctg gaccttcaac ctctcgatcg gcgagagcct
     4501 cgagctggaa tggcccgatt tcgtcccgcg cgagcggctc gatgcctggc tcgccgcccg
     4561 cgccggcgag gacttctcgg gcgacatcta tgcccgccgg ttggatgtcg cgtcttgagt
     4621 gtcgagcccg ggacctactg gccgaccctg ctccaggaac gccgcctgcc ggcagcggcc
     4681 gcggccgacc cgctcgcctg cgcctatctc gacttcgccc ggcccgagcg ggtcgtgctc
     4741 ggccatctcg gccagagcct cgacggctac gtcgccacgc tcgccggcca ttcggccaac
     4801 atgacgggtg ccgccaacat ccgccacctg catcgcctgc gggcgcttgc ggacgccatc
     4861 gtcgtcggcg ccggcaccgt cctcaacgac gacccgcggc tcaccacgcg gctcgtcgcc
     4921 ggcccgtcgc cggtccgcgt cgtcctcgac aaccgccgcc gcctgggcgc ggactatcgt
     4981 ctcttccggg aaggcccgcc gaccctcgtc ctctgcgccg ccgacgcggc cgacggcgcc
     5041 acgctcggcc gggcggaaat cgtgccggtc gagacggcgg acggcagcgt ggcgccggca
     5101 gccgtgctcg aggcccttgg cacccgcggc ctgaagcggg tcttcgtcga gggcggtggc
     5161 gtcaccgtct cgcgcttcct cttcggcggc tgcctcgaca tcctcgagat ctgcgtctcg
     5221 ccgctcgtcc tcggcagcgg ccgccggggc ctcgccggcc cggcgccgga acgcaccgac
     5281 gaggcactca ggctcgaggg ctgccgggcg atccccatgg gcgccgacat gctcttccac
     5341 cagaagctgc ggccgggcta aagtcgcctt caagtcgggc aagggcccgc tcggcagtca
     5401 gggagggaac ctcatgcggc gcacgttcgt ccgggcggcg cagccggacg aagcggctgc
     5461 actctccgcc ctggcgctgc ggagcaaggc ccactggggc taccgcccgg ccttcatggc
     5521 gatgtgtgtg gccgagctga cggtgacgcc cgagctgatc gacgaagggc tggcgctggt
     5581 cgccgagcgc tccggccgga tcgccggctt catcgtctgc acgcccgtcg atgccgcctc
     5641 ggtcgagctc gacatgatct tcgtcgagcc cgaggcgatc ggccagggga tcggccggct
     5701 gctggtcgac gctgccttcg ccgggctcgc tcggcgcggc gtccggcggg tggaggtcgc
     5761 ctcggacccg catgccgagc ccttctatcg aagctgcggc ttcgagcccg tcggcaccgt
     5821 gccctcgggc tcgattcccg gccggttcct gccacggcta tgcttcgcgc tcgatgagcg
     5881 ctattgcgtt gcgcccaagg aggcacgctg atgtatcgcg acaagtacga cctgaccggg
     5941 cgcgtcgccg tcgtcaccgg cggcggccgc ggcatcggct tcgagagtgc ccgagccctc
     6001 gccgagtgcg gcgcccatgt ggtcatcgcc gagctcgacg cggcgaccgg gccgcaggcc
     6061 gcggccgagc tcgagcggct cggctgctcg gccgagttcg tgcccctcga cgtcacgcag
     6121 tccgccgccg tcaccgccac cgccgacgcg ctgcaggagc ggcacggccg gatcgacatc
     6181 ctcgtcgcca atgccggcat cgccaagggc acgcccggcg aggacatgtc ggacgaggaa
     6241 tggctgctcg tcctcgacat caacctgaac ggcgtcttct ggtgcaaccg cgccttcggc
     6301 cggcacatgc tggcacgggg gcgcggcagc atcgtcaaca tcggctcgat gagcggcctc
     6361 atctcgaaca agccgcagcc gcagagcgcc tacaacgcgt cgaagtcggg cgtgcacatg
     6421 ctgaccaggt cgctcggcgg cgagtgggcg acgcgcgggg tcagggtcaa tgccgtggct
     6481 cccacctaca tcgccaccga gatgacccgc atgggtcgcg agaatcccga atggctgccg
     6541 acctggctcg agatgacgcc gatgcgccgg ctgggcgagc cgcacgagat cgcctcggcc
     6601 gtgctcttcc tcgcgtccga tgcatcgagc ctgatgaccg gcagcgtcgt cgtcgtcgac
     6661 ggcggctaca ccatctggtg aagagaggaa gcatgcccgc ctacgtcatc gcgacgatcg
     6721 aggtcaccga ccccgaggcc tacgaggcct acaagacggc cgccgcggcg gcgatcgcga
     6781 agcatggcgg gcgctatctg gcgcgcggcg gccgcagcca cgtggccgag ggtgctttcc
     6841 cgggcagccg cttcgtgctg ctcgagttcg ccgactacgc caccgcccag ggcttcgtcg
     6901 cgtcggcgga ctatcaggcc gccaaggcgc tgcgcgccgg ggccgcggaa atgaacatgg
     6961 tcatcatcga ggggtacgtg ccgtgaagct caaggacagg gttgccgtga tcaccggggg
     7021 cgcgcgcggc atcggccggg cgatcgccga gcgctatgcc gacgaggggg cgagggtggt
     7081 cgtcgccgac atcctcgagg ccgaggccaa agcgacggcc caggccatcg gccgaggcgc
     7141 gttcggcctg aaggtcgacg tcaccgacca ggcctcgatc gatgccatgg tgaaggccgt
     7201 ggtcgacgag gcgggccgga tcgacatcct cgtcaacaac gccgccatct tcaacatggc
     7261 gcccgtgctc gaggtcaccc gcgaggactg ggaccgcaat ttcgacatca acaccaaggg
     7321 tctcttcttc acgctccagg cggtcgcccg gcggatggtc gagcaggcgg cggccgaccc
     7381 ggcctggcgc ggcaagatca tcaacatggc gagccaggcg ggccggcggg gcgaatcgct
     7441 ggtgtccgcc tattgcgcgt ccaaggccgc cgtcatctcg atcacccaga gcacggccaa
     7501 gggcctgatc ccgcaccgca tcaacgtcaa cggcatagcg cccggcgtgg tcgacacgcc
     7561 catgtgggac gaggtggacc ggctcttcgc caagtacgag aacctgccga tcggcgagaa
     7621 gaagcggcag gtgggcgagg ccgtgccctt cggccgcatg ggcgtgcccg aggaccatgc
     7681 gggtgccgcc gtcttcctcg cctccagcga tgccgactac gtgctggccc agacgctcaa
     7741 cgtcgacggc ggcaactgga tgagctgaag cgacggagca gcacgatgcc cttcacgctc
     7801 ggcgtcaaca ccaacgcctt cgtcaaccgg atcgtcgagc cggaagagct ggtgcgcacc
     7861 gtggtcgaaa agatcgggct caggcgcatc cagctcacgc ccgagatcct gagccccgac
     7921 tggccggctt cggtcatcgc cgcccaggtg cgccgctgga acgcggcctg cgagaagcac
     7981 ggggccagga tcaccacgac cttcaccggc aactacacgc gcctcaacag cctctgtcac
     8041 cccgaccccg acgtgcgcgc ccactggcgg gcctggctcg ggcggctgat cgacatcacc
     8101 gccgacctcg gcggtgccgc cacgggcagc cagatcggca tcctgaccta tgccgacgac
     8161 cgcgaccccg cccgccgcgc cgcccgcttc gccgaggccc gcgaccactg gcacgcgctg
     8221 gccgggcacg ccaaggaacg cggcctcgcg tgcctgatgt gggagcccat gtcgatcagc
     8281 cgcgagttcg gccagaccat cgaggcctgc aggcgcgacc acgccgccct caacgagggc
     8341 tcgcctttgc cgatcctgat caacgccgac atcgaccacg gcgacgtcac ctcatccgac
     8401 cccgccgaca ccgaccccta tgcctgggcc gaggccctgg cccaggtgag ccccgttgtc
     8461 cacatcaagc agagctccat ggacaagggc ggccatcgcc ccttcaccgc cgcctacaac
     8521 gccaagggcc gcgtcgtgcc cgaacgcctg atccaggccc tcgaacgcgg cggcggccgg
     8581 gacaacgagc tcgtcttcga gttcaccttc cgcgagcgcg agccgaccga cagcgaggtc
     8641 gtggcggcgc tgaaggagtc ggtcga
//
LOCUS       DAOTFI010000212         8654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_9766_length_8654_cov_7.621933, whole genome shotgun sequence.
ACCESSION   DAOTFI010000212 DAOTFI010000000
VERSION     DAOTFI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9766_length_8654_cov_7.621933"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="P5558_18490"
     CDS             complement(<1..703)
                     /locus_tag="P5558_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26364.1"
                     /translation="MRAFPGLTSLLAALGLLWAAPAGACPGADAPCRVADGEYFIRLP
                     ETGSTVGRPALMVHLHGAGGQAADVVRGAGFLDVFLGRGYAVLAPQGLAREGGRGPNW
                     GVSDGRPHPRDDLAFIGEVIDDAVGRFDLDRTRVLLTGFSRGGSLVWDIACQAPAMAS
                     AYAPIAGGFWRPHPASCKGPVRLLHTHGFTDGTVPLEGRPLRGGAVAQGDIYEGLQLW
                     RRTNGCGDRADWHDTS"
     gene            861..1814
                     /locus_tag="P5558_18495"
     CDS             861..1814
                     /locus_tag="P5558_18495"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRY26365.1"
                     /translation="MTVRANERVAWFNGEFMPESQVRIPFRDSSWLYGDGCFDLTRTF
                     GHRLFKVREHVDRLYRSLKYLRIDPGFGPERMCALTEELFERNRHLLGADDDYWVGQR
                     ISRGVKEVEGDNIDHHGPNIVLECNPLPLGKRAKLFKDGIRVVVPSHRRIPPDSLTPR
                     AKTHNYLNLIVADQEVQSIDPEAWAVLLDVNGNLCEGMGSNIFIVRDGEILTPREKFV
                     LPGVSRQTAIDLARAEGLTLREADLDLYDAYNADEAFLTSTSLCVCPIVKVNGVKIGP
                     EGQVWGPVTTRITEAYQRLVDHDFVGQYLKHYVEGAEARAF"
     gene            1843..2595
                     /locus_tag="P5558_18500"
     CDS             1843..2595
                     /locus_tag="P5558_18500"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY26366.1"
                     /translation="MTFSLAGVLAGSLAMGTAGILRGFTGFGTALAGVPLLSLVFAPR
                     LAVPTIMALQIATGLQNLHADGRHVEWRTLLRLLPGAVVGIVPGLWLLLWLSADAARL
                     AIGLIVVLTSLLLQRGWRLERLPGTGGLLALGVLSGVLNGLAAIAGPPVIALFFALRR
                     SPEVTRASLAAFFLCNGVLGLSLALGKGLIAPGELWLAALLCPAMILGTTLGGRLFTG
                     DLKRHYRLVGLVALLAVGLAAAARGAIGLWLG"
     gene            complement(2592..3695)
                     /gene="ribA"
                     /locus_tag="P5558_18505"
     CDS             complement(2592..3695)
                     /gene="ribA"
                     /locus_tag="P5558_18505"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRY26367.1"
                     /translation="MSEIFQPDMQDFIKVAAADRFLRGGMACRVIDAAGRVTVKALES
                     MDDASLGTQLAGGAATLLLSARRAAALGIRPAGPDAVRIALPAGTAVEQVRALVDPTR
                     LMAALAETPASPASPADVAAIELCKIANLLPACVVVAGTEPAALELDAATVMAYRTAA
                     AGSFERITEARVPLADAADARFVAFRSRLGGPQHYAILVGRPEAFAAPLCRLHSECFT
                     GDLFGSLRCDCGEQLKGAIRRMDEAGGGVLLYLAQEGRGIGLVNKLRAYTLQDSGVDT
                     VDANTHLGFEPDERDFIAAATMLRDLGFSAVRLLTNNPDKVTQLAERGIQVTERVEHS
                     FAANRHNRFYLETKASRSGHLLRLPDLPRRGAA"
     gene            3906..4895
                     /locus_tag="P5558_18510"
     CDS             3906..4895
                     /locus_tag="P5558_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HRY26368.1"
                     /translation="MKLEAEAYWAVAPGRGEIRPVALAAPGAGEVLVETRWSAISRGT
                     EALVALGRVPASQAQAMRCPFQEGSFPFPVKYGYCSVGTVALGPEALRGRTVFCLYPH
                     QTRYVVPASAVHVLPDGLPEERAVLAANMETALNAVWDAGLLPGERIVVMGAGVVGCL
                     IARLAVQLPGAEVTLVDIDPAKAGIAEALGLPFRKDADGLEPADCVVNASGAGAGLAA
                     GLALAGIEARIVEVSWHGDAAVTLPLGEAFHSRRLRVLGSQVGMIAPAMRPRFDHRRR
                     LAKAMELLASDPALDALIDGESPFHELPELMPALAGAGSGVLCHRVSYRKREG"
     gene            4898..5296
                     /locus_tag="P5558_18515"
     CDS             4898..5296
                     /locus_tag="P5558_18515"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRY26369.1"
                     /translation="MYKVRVRDRMMIAHSFEGEVFGPAQRLHGATFVVDVEFSGRDTN
                     EDNIVVDIGRAIDRLKAILADLNYQNLDGIEAFRGQNTTTEFLARYIFERMKEAIGQG
                     LLGPGSEALAGLRVELAESDVAWASYEAPL"
     gene            complement(5312..5641)
                     /locus_tag="P5558_18520"
     CDS             complement(5312..5641)
                     /locus_tag="P5558_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26370.1"
                     /translation="MADLIGENAAPGRAAAHLMYALYAVALFTALPMLAGVIIAYVGR
                     GSAEPVYRSHLDWGITTFWVALVIGIIGFVLLFILIGYLVLGLLWLWTAYRIIRGWLR
                     LLDNRGI"
     gene            5695..6756
                     /locus_tag="P5558_18525"
     CDS             5695..6756
                     /locus_tag="P5558_18525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY26371.1"
                     /translation="MPETKLTLAVPGGFDRLTGGTIYDGRVADELEGRGWAIHRLALP
                     DGYPLPDAAALATTRQLLTALPEGCRLVVDGLALGVLPELAAELAPRLELIALVHHPL
                     ALETGLDAATAERLRASERRALAHAARVICTSRTTRLALARDYAVPEARITVAPPGTD
                     RRPAARGSGGPGLHILNVGSVIPRKDQRTLVAALAELGDRRIRTSIAGSLERDPATAL
                     ALRALIDKTGQRDRTALLGELEAGLLDEALDTTDLFVSTALHEGYGMALSEALAAGIP
                     ALVARGGAVVEAVPESAALFFEPGDVEGLGRLIGALADDAIALDRLREGARRASERLP
                     TWADTARLVAEALLREPAP"
     gene            6753..7559
                     /locus_tag="P5558_18530"
     CDS             6753..7559
                     /locus_tag="P5558_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26372.1"
                     /translation="MSGFAHDWLALREPYDHAARSTDLGQRLAGWAAGRSRLGVVDLG
                     SGTGSNLRATAPALACPQAWTLVEHDPALIAAGTPALAGLPANVTAAYAGIDLARELA
                     TALPAGTDLVTAAAFLDLVSAAWLDELVALVLERRAALHVVLTCDGGLRWRPGDLFDG
                     EVKRLFEAHQRTDKGFGSALGPTAVDALEARLRAGQGELHVAASDWRLGYADQAIQAA
                     LLDGYARSATEIAPAQAAEIADWQAQRARQIEAGRSEHRVGHKDLLWLPE"
     gene            complement(7561..8238)
                     /locus_tag="P5558_18535"
     CDS             complement(7561..8238)
                     /locus_tag="P5558_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26373.1"
                     /translation="MSEHALIVGAGHGLSAAFARRLALDGVAVSLAARDTAKLDGLAR
                     ELDARTFACDAGDAASVAGLFAELDAAGRTPTIVHYNPSARTRAPFVELVPEDVERAL
                     RITAFGAFLVAHQAVPRMLRQGGGTLLFTGASASLKGYPRSAPFAMGKFALRGLAQSL
                     ARELQPSNIHVVHIVVDGGIAPQGGAPGEDQLDPSAIAETMHHLVRQPRSAWSWEIEL
                     RPWVERF"
     gene            8368..>8654
                     /locus_tag="P5558_18540"
     CDS             8368..>8654
                     /locus_tag="P5558_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY26374.1"
                     /translation="MAGARQFDESQVLDAMMRVFWEKGFEATSIDDLVRATGLQRGSL
                     YNAFGDKAAMFCRSLRLYQDEVHAANLHALRGDDPRAALTRLFQLELKSAR"
BASE COUNT         1226 a         3083 c         3124 g         1221 t
ORIGIN      
        1 ctgaggtgtc gtgccagtcg gcgcggtcac cgcagccgtt cgtccggcgc cagagttgca
       61 acccctcgta gatgtcgccc tgcgccacgg caccgccgcg gagcggccgc ccctcgagcg
      121 gcacggtgcc gtcggtgaag ccgtgggtgt ggaggagccg gaccgggccc ttgcaactcg
      181 ccggatgcgg ccgccagaag cccccggcga tgggcgcata ggcgctggcc atggccggcg
      241 cctggcaggc gatgtcccag acgagggagc cgccgcgcga gaagccggtc aagaggacgc
      301 gcgtgcggtc gagatcgaag cggccgacag cgtcgtcgat cacctcgccg atgaaggcga
      361 ggtcgtcgcg cgggtggggg cgcccgtcgc tgacacccca gttcggcccc ctgccgcctt
      421 ccctggcgag gccctgcggc gccagcacgg catagccccg gcccagaaaa acatcgagga
      481 agccggcgcc gcggacgacg tcggcggcct ggccgccggc cccgtgcaga tggaccatca
      541 aggccggtcg gccaacggtg ctgcccgttt cgggcaggcg gatgaaatac tcgccgtcgg
      601 caacgcggca gggcgcgtcc gcacccggac aggccccggc cggggccgcc cagaggagac
      661 cgagcgctgc gagcagagag gtcaggccag gaaaagcccg catgggctgg tctcctcgga
      721 tcggcagaaa acgtgcctgt ggggccggtg ccgccgtggc catggccaag gcgagccggt
      781 cgtgcgttat tgacggttgc cagccccggc ggttccgcgc atcagacggt ctggtcgaga
      841 aaaatcgcgg agggaatccc atgacggttc gggcgaacga gcgggtggcc tggttcaacg
      901 gcgaattcat gccggaatcg caggtgcgga tcccgtttcg cgattcgagc tggctctacg
      961 gcgacggctg cttcgacctg acccggacct tcggccatcg cctgttcaag gtgcgggagc
     1021 atgtcgaccg gctctaccgc tcgctcaagt atctgcgcat cgacccgggc ttcgggccgg
     1081 agcgcatgtg cgcgctgacc gaggagctct tcgagcgcaa ccgccatctg ctcggcgccg
     1141 atgacgacta ctgggtcggc cagcggatca gccgcggggt caaggaggtc gagggcgaca
     1201 atatcgacca tcacggcccc aacatcgtgc tcgagtgcaa tcccctgccg ctcggcaagc
     1261 gggccaagct cttcaaggac ggcattcgcg tggtcgtgcc ttcgcaccgg cgcatcccgc
     1321 cggattcgct caccccccgc gccaagacgc acaactacct gaacctgatc gtcgccgacc
     1381 aggaggtgca gagcatcgac cccgaggcct gggccgtgct gctcgacgtg aacggcaatc
     1441 tctgcgaggg catgggctcg aacatcttca tcgtccgcga cggcgagatc ctgaccccgc
     1501 gcgagaagtt cgtgctgccc ggcgtcagcc ggcagaccgc gatcgacctc gcccgggccg
     1561 agggcctgac cctgcgcgag gccgatctcg acctctacga cgcctacaac gccgacgagg
     1621 ccttcctcac ctcgacctcg ctctgcgtct gcccgatcgt caaggtgaac ggcgtcaaga
     1681 tcgggccgga gggccaggtc tgggggccgg tcaccacgcg gatcaccgag gcctaccagc
     1741 gcctcgtcga ccacgatttc gtcggccagt atctcaagca ctatgtcgag ggtgcggagg
     1801 cccgcgcctt ctgagcgctg ctcgcccgtc gacgagccgg acgtgacgtt ctcgctcgcg
     1861 ggcgtcctcg ccggctcgct ggcgatggga accgccggca tcctcagggg cttcaccggc
     1921 ttcggcacgg ccctcgccgg cgtgcccctc ctgagcctcg tcttcgcccc gcggctcgcc
     1981 gtgccgacca tcatggcgct gcagatcgcg accgggctgc agaacctcca tgcggacggg
     2041 cggcacgtcg agtggcggac cctgctgcgg ctcttgcccg gtgccgttgt cggcatcgtg
     2101 cccgggctct ggctcctgct ctggctcagc gcggatgccg cccggctcgc catcggcctg
     2161 atcgtcgtgc tcacgtcact gctgctgcag cgcggctggc ggctggagcg gctgcccggc
     2221 acgggcgggc tcctggcgct cggcgtgctt tcgggcgtgc tcaacggact cgccgcgatc
     2281 gccggcccgc cggtcatcgc cctcttcttc gcgttgcgtc gctcgcccga ggtgacccgc
     2341 gcctcgctcg ccgccttctt cctctgcaac ggcgtgctgg ggctgagcct ggcgctcggc
     2401 aaggggctga tcgcgccggg cgagctctgg ctcgcggccc tgctctgccc ggcgatgatc
     2461 ctgggcacga cgctcggcgg gcggctcttc accggcgacc tgaagcgaca ctaccggctg
     2521 gtcggcctcg tggccttgct cgccgtcggg ctcgccgccg cggcacgggg cgccatcggc
     2581 ctctggctcg gctaggcggc accccgccgc ggcaggtcgg gcagccgcag gagatggccg
     2641 ctgcgcgacg ccttggtctc gagatagaag cggttgtggc ggttggcggc gaagctgtgc
     2701 tcgacccgct cggtgacctg gatgccgcgc tcggcgagct gggtgacctt gtcggggttg
     2761 ttggtcagga gccggacggc gctgaagccg aggtcgcgca gcatggtggc ggcggcgatg
     2821 aagtcgcgct cgtccggctc gaagcccaga tgtgtgttgg catcgacggt gtcgacgccg
     2881 gagtcctgca gcgtgtaggc gcgcagcttg ttgacgaggc cgatgccccg gccttcctgc
     2941 gccagataga ggagcacgcc gccgccggcc tcgtccatcc ggcggatcgc acccttgagc
     3001 tgctcgccgc aatcgcagcg cagcgagccg aagagatcgc cggtgaagca ctccgaatgc
     3061 agccggcaga ggggggcggc gaaggcctcc gggcggccga ccaggatcgc gtagtgctgc
     3121 ggcccgccga gcctggagcg gaaggccacg aaccgtgcgt cggcggcgtc cgccagcggc
     3181 accctggcct cggtgatccg ctcgaagctg ccggcggccg ccgtgcgata ggccatgacg
     3241 gtcgccgcat cgagctcgag ggcggcgggc tcggtccccg cgacgacgac gcaggccggc
     3301 aggaggttgg cgatcttgca gagctcgatg gccgccacat cggccggcga agcggggctg
     3361 gcgggcgtct cggcgagagc cgccatgagc cgggtcggat cgacgagggc gcggacctgc
     3421 tcgacggcgg tgccggcagg aagtgctatc cgcaccgcgt cggggccggc cggccggatg
     3481 ccgagcgccg cggcccgccg ggcgctgagc agcagggtgg cagcgccacc ggcgagctgg
     3541 gtcccgaggc tcgcatcgtc catgctctcg agcgccttga ccgtgacccg gccggccgcg
     3601 tcgataaccc ggcaggccat gccgccgcgc aggaatcgat cggcggctgc caccttgatg
     3661 aaatcctgca tatcaggctg gaaaatctcc gacatcggcg cctcttgctg gtctagcccg
     3721 gtatacgatg cgtttccctg caccgatcaa cgctgctccg ccaaatgagc tcgggcgccg
     3781 acgacgcgct gaagagttaa gtcaggaaca tctttttgcc agtgggttac ggccgtgtca
     3841 cgttcgacga accgaatggt cgcaggccgg ccgggccgat cgcgccgaat tgaccgggag
     3901 tgaacgtgaa gctcgaggcc gaggcgtatt gggctgtcgc accgggccgc ggcgagatcc
     3961 gccccgtcgc cctggcggcg ccgggcgcgg gcgaggtgct ggtcgagacg cgctggtcgg
     4021 cgatcagccg cggcacggaa gcgctcgtcg ccctcggccg cgtgccggcc tcgcaggccc
     4081 aggccatgcg ctgcccgttc caggagggca gcttcccctt tccggtcaag tacggctatt
     4141 gcagcgtcgg caccgtggcc cttggccccg aggcgctgcg cggccgcacc gtcttctgcc
     4201 tttacccgca ccagacccgc tatgtcgtgc cggccagcgc cgtgcacgtc ctgccggacg
     4261 gcctgccgga agagcgcgca gtgctcgcag ccaacatgga gaccgcgctc aacgccgtct
     4321 gggatgccgg cctgctgccg ggcgagcgca tcgtcgtcat gggtgcgggc gtggtcggct
     4381 gcctgatcgc ccggctcgcc gtgcaactgc cgggggccga ggtgacgctg gtcgacatcg
     4441 atccggcgaa ggccggcata gccgaggccc tgggcctgcc gtttcgcaag gacgccgacg
     4501 ggctcgagcc cgccgattgc gtcgtcaacg cctccggcgc cggagcgggg ctcgccgccg
     4561 gcctcgccct cgccggcatc gaggcgcgga tcgtcgaggt gagctggcac ggcgatgcgg
     4621 ccgtcaccct gccgctgggc gaggctttcc acagccgccg gctgcgcgtc ctcggctccc
     4681 aggtcgggat gatagcgccc gcgatgcggc cacgcttcga ccaccgccgg cggctggcca
     4741 aggccatgga gctgctcgct tccgaccccg ccctcgacgc gctgatcgac ggcgagagcc
     4801 cgttccacga gctgccggag ctgatgccgg ctcttgccgg cgcgggctcc ggcgtgcttt
     4861 gccaccgcgt gagctaccgc aagagggagg ggtgagcatg tacaaggtcc gcgtgcgcga
     4921 ccggatgatg atcgcgcaca gcttcgaggg cgaggtgttc ggcccggcgc agcggctgca
     4981 cggtgcgacc ttcgtcgtcg atgtcgagtt cagcggcagg gatacgaacg aggacaacat
     5041 cgtcgtcgac atcggccggg cgatcgaccg gctcaaggcg atcctggccg acctcaacta
     5101 ccagaacctc gacgggatcg aggccttcag gggccagaac acgacgaccg agttcctcgc
     5161 ccgctacatc ttcgagcgca tgaaggaggc gatcggccag ggtctgctcg ggccgggcag
     5221 cgaggcgctg gcggggctca gggtcgagct ggccgagagc gatgtcgcct gggccagcta
     5281 cgaggcgccg ctctgagagc ggtcggggcc gtcagatgcc ccggttgtcc aggagcctga
     5341 gccagccgcg gatgatgcgg taggcggtcc agagccagag caggccgagc acgagatagc
     5401 cgatcaggat gaagagcagg acgaagccga tgatgccgat caccagcgcc acccagaagg
     5461 tggtgatgcc ccagtcgaga tggctgcggt agaccggctc ggcgctgccg cggccgacat
     5521 aggcgatgat gacgccggcg agcatcggca aggcggtgaa gagcgccacg gcatagagcg
     5581 cgtacatgag atgggcggcc gcccgcccgg gcgccgcgtt ctcgccgatc agatccgcca
     5641 tcgctcacct ctccgtgccc tttccaccgg cgctcgctag cacatgtgac ggccatgccc
     5701 gaaaccaagc tgaccctcgc cgttcccggc ggcttcgacc ggctgaccgg cgggacgatc
     5761 tacgacgggc gcgtcgccga cgagctggaa ggccggggct gggccatcca tcgcctggcc
     5821 ctgccggatg gctacccttt gccggatgcg gcggccctgg cgacgacccg ccagctcctc
     5881 acggccctgc cggaaggctg ccgcctcgtg gtcgacgggc tggcgctcgg cgtgctgccg
     5941 gagctggcgg ccgagctcgc cccgcggctc gagctgatcg ccctcgtcca tcacccgctg
     6001 gccctggaga cggggctcga cgcggcgacg gccgagcggc tgcgcgccag cgagcggcgc
     6061 gccctcgccc atgcggcccg ggtgatctgc acgtccagga cgacgcggct ggcactcgcc
     6121 cgggactacg ccgtgcccga ggcccggatc accgtggcgc cacccggcac cgatcgccgg
     6181 ccggccgccc ggggctccgg cgggccgggg ctgcacatcc tgaacgtcgg cagcgtcatc
     6241 ccgcgcaagg accagcgcac gctggtggca gcgctggccg agctcgggga ccggcggatc
     6301 aggaccagca tcgccggcag cctggagcgg gatccggcga ccgccctagc gctccgcgcg
     6361 ctgatcgaca agaccggcca aagggaccgc accgcgctgc tgggcgagct ggaagccggg
     6421 ctgctcgacg aggcgctcga cacgaccgac ctcttcgtct ccaccgccct ccacgagggc
     6481 tacggcatgg cccttagcga ggcgctcgcc gctggcatcc ccgccctcgt cgcccgtggt
     6541 ggggcggtgg tcgaggccgt ccccgagagt gccgccctct tcttcgagcc gggcgacgtc
     6601 gaggggctcg gccggctgat cggtgcgctc gccgacgacg ccatcgccct cgaccgcctg
     6661 cgtgagggtg cccgccgggc gagcgagcgc ctgcctacct gggcggacac cgcccggctg
     6721 gtggccgagg cgctattgcg cgagcccgcc ccgtgagcgg cttcgcccat gactggctgg
     6781 cgctgcgcga gccctacgac catgcggcac gatccaccga cctcggccag cggctcgccg
     6841 gctgggccgc cggccggtcg cggctcgggg tcgtcgatct cggcagcggc accggcagca
     6901 acctgcgcgc caccgccccg gccctcgcct gcccgcaagc ctggacgctg gtcgagcacg
     6961 acccggcgct gatcgccgcc ggcacgccgg ccctggcggg gctgccggcg aacgtgacgg
     7021 ccgcctatgc gggcatcgac ctcgcccggg agctcgccac ggcccttccc gccggcaccg
     7081 acctcgtcac cgcggcggcc ttcctcgacc tcgtgagcgc tgcctggctc gacgagctgg
     7141 tggccctggt cctggagcgc cgggcggcgc tgcatgtcgt cctcacctgc gatggcggtc
     7201 tccgctggcg accgggcgac ctcttcgatg gcgaggtgaa gcggctcttc gaggcgcacc
     7261 agcgcaccga caagggcttc ggttcggccc tcggcccgac cgccgtcgac gcgctcgagg
     7321 cacggctacg ggccggccaa ggcgagctgc atgtcgcggc cagcgactgg cggctcggct
     7381 atgcggatca ggcgatccag gcggcgctgc tcgacggcta cgcccggtcc gcgaccgaga
     7441 tcgcgccggc ccaggccgcc gagatcgccg actggcaggc ccaacgcgcc cggcagatcg
     7501 aggccgggag gtccgagcat cgcgtcggcc acaaggacct gctctggctg ccggaataga
     7561 ctagaagcgc tccacccacg gccgcagctc gatctcccag gaccaggcgc tgcgcggctg
     7621 gcggacgagg tggtgcatgg tctcggcgat cgccgaggga tcgagctggt cctcgccggg
     7681 ggcgccgccc tggggggcga tgccgccgtc gacgacgatg tgcaccacgt ggatgttcga
     7741 gggctgcagc tcgcgggcga ggctctgggc gaggccgcgc agcgcgaact tgcccatggc
     7801 gaagggggcc gagcgcggat agcccttcaa gctggccgag gcaccggtga agaggagcgt
     7861 gccgccaccc tggcggagca tgcgcggcac ggcctgatgc gccacgagga aggcgccgaa
     7921 ggcggtgatc cgaagcgccc gctcgacgtc ctcgggcacg agctcgacga agggggcgcg
     7981 cgtccgggca ctcgggttgt agtggacgat ggtcggcgtg cgcccggccg cgtccagctc
     8041 ggcgaagagc cccgccaccg aggcggcgtc gccggcatcg caggcgaagg tccgcgcatc
     8101 cagctcgcgt gcgaggccgt cgagcttcgc cgtgtcgcgc gcggcgagcg agacggcgac
     8161 cccgtccagc gccagccggc gcgcgaaggc ggccgacagc ccgtgtcccg cacccacgat
     8221 cagggcatgc tcgctcatcg cctccacttc ccgctcgttt tcgcctgccc gtcactatag
     8281 ggcgtcttcg cggcacggcc gcgatccttg accgatcggt caaaaggcct agatgcagaa
     8341 ggctggatcc gggggaggaa aagggtgatg gcgggcgcac ggcagttcga cgagagccag
     8401 gtgctcgacg cgatgatgcg ggtcttctgg gagaagggct tcgaggccac gtcgatcgac
     8461 gacctcgtcc gggccaccgg cctgcagcgc ggcagcctct acaacgcctt cggcgacaag
     8521 gccgccatgt tctgccgctc cctgcggctc taccaggacg aggtgcatgc agcgaacctg
     8581 cacgcgctgc gcggcgacga cccgcgggcg gcgctcaccc gcctgttcca gctcgagctg
     8641 aaatcggcgc gcaa
//
LOCUS       DAOTFI010000213         8434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10120_length_8434_cov_7.116720, whole genome shotgun sequence.
ACCESSION   DAOTFI010000213 DAOTFI010000000
VERSION     DAOTFI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10120_length_8434_cov_7.116720"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="P5558_18545"
     CDS             complement(<1..474)
                     /locus_tag="P5558_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY26375.1"
                     /translation="MTLLLLLAGLVLVILTGLPVFAALGLFGGTLMVVDEGSLAGLGE
                     IVFGKLNIYLLVAIPLFTLMAHFMIRGRIVDDLYGTAHTLLRHLPGGLGVATVAACTI
                     FAAISGSSVATALTIGAVAIPQMIRYGYSKPAAYGVVGGGGTLGILIPPSGPMVLY"
     gene            complement(471..980)
                     /locus_tag="P5558_18550"
     CDS             complement(471..980)
                     /locus_tag="P5558_18550"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY26376.1"
                     /translation="MACLCLVGTGLAAALLLVSLALVAWSVMMRYFLNQPIPWVDELV
                     GYLLVGLVMLAAADALRRGEHISVDLLTDRLGPRGRRLTAAAGQLAVLVAGLALLIGG
                     WQTAAFSKLLGIYSTGYLAVPVYLPQLLIPLGGLLLAVAAAVGLLRMATGQSPTSGPA
                     EPGAGGLRP"
     gene            complement(1043..2029)
                     /gene="dctP"
                     /locus_tag="P5558_18555"
     CDS             complement(1043..2029)
                     /gene="dctP"
                     /locus_tag="P5558_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY26377.1"
                     /translation="MKRKSTLAVAALALTLPLGSLHAETVFRISHQLPPAHHIAQLVE
                     AFAADVEARTDGAVDVQIFGASQAYKPDQHHPAVARGEIEGAIAVNFQWGNTIPEMNV
                     VTIPYFFTDLARIEAFPGSKAADLLDAKLLEKGVENIAWLYTTRQSIFTSSKRPLITV
                     EDFEGVKIRGLNKLVDEGLVAAGAAPAAMPGSEVYQALQTGVIDAGLTDVSAAFSRKY
                     YEVQEFGTVAPFFTVYFHLFVNPAWYAGLPEAERAAIAEAADAAELESIPLTESTAEA
                     AIDELQKAGMKLHIQTPEESAAFSAVMQPPVMEAFKASSPDAEAIIEAVEQL"
     gene            complement(2068..2970)
                     /locus_tag="P5558_18560"
     CDS             complement(2068..2970)
                     /locus_tag="P5558_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRY26378.1"
                     /translation="MRPTTRFRRLVDAPGILVLPGIQDALTARIAEAAGFAAVTCGGY
                     AATAALLGQPDTSQLGLMEMAEHYARICDAVSVPVFADADTGFGNVTNVRRAVRAYER
                     AGVAGLFIEDQLFPKRCGHMAGKAVVPAEEMVGKLKAALDARRDADLVIMARTDALAI
                     NGLDDAIERGQLYRAAGADMVFVEAPRDIDQMRRICAEIDAPCMANNIEGGLTPFLPA
                     AELEAIGYAMVAFPVATTYAIAATIGRLMQTLHATGTTADAADAMLDFTAFNALVGLP
                     ELRAAEERDLAFARRLMDAGAPRR"
     gene            complement(2892..3482)
                     /locus_tag="P5558_18565"
     CDS             complement(2892..3482)
                     /locus_tag="P5558_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26379.1"
                     /translation="MLLLVIGAVDKRWIGRHKRSKNFVARDWQLLLDHMGNATLVTTP
                     HILAETSNLLRKGGLAAPATDQLMSRLAQFISLAREQHIPARDVIGTQLYLRLGLTDA
                     AILSIEEPDVHLLTTDVNLYIAAIRQGRKATNFNHLRDFAGASRLAPVPAAIYNPLTD
                     NHKLHATGAEPCARPPAFAVSSTHPGSSSCRASRTR"
     gene            complement(3509..3739)
                     /locus_tag="P5558_18570"
     CDS             complement(3509..3739)
                     /locus_tag="P5558_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26380.1"
                     /translation="MFDDEGISNIGLEEIEFVDDGGLWQVTVGFSRAWDQPAGVAAAF
                     GGRLGRTFKVVTLKDNDGRVLSVKHRELVLAD"
     gene            complement(3869..5122)
                     /locus_tag="P5558_18575"
     CDS             complement(3869..5122)
                     /locus_tag="P5558_18575"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRY26381.1"
                     /translation="MIEDNRNLTEDRPTFVTHLECSMTGERYEADRLHNLSAAGKPLL
                     VRYDLDGVKGALTKAKLADRPMDLWRWRELLPVRRTENIVSLGEIATPLIELPTLAST
                     HGATGSLIVKDEGRLPTGSFKARGLVMAVAMAKELGITHMAMPTNGNAGAALSAYASH
                     CGIKTTIFCPADTPETNISEMALQGADVYRVNGLIDDCGKIVGAGKETVGWFDTSTLK
                     EPYRIEGKKTMGLELAEQLGWELPDVIFYPTGGGTGLIGMWKAFAELEAIGFIGTKRP
                     RMVAVQATGCAPMVKAYEDGVEHAPRWENAATIASGIRVPQAVGDFLILRAVRESGGF
                     AIAVDDDAIQAGLDEVAGKEGFLLCPEGAATYAAWQTSLKDGRIQPTDRSVLFNCATG
                     LKYPLPKIEKSLDRHGQIDWAQFRR"
     gene            complement(5119..5295)
                     /locus_tag="P5558_18580"
     CDS             complement(5119..5295)
                     /locus_tag="P5558_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26382.1"
                     /translation="MRTRVESCCIASREEAAMAMPLGADLCSGVGRDGRLDKSLRHRF
                     MTAIQQADREGCQA"
     gene            5355..6509
                     /locus_tag="P5558_18585"
     CDS             5355..6509
                     /locus_tag="P5558_18585"
                     /inference="COORDINATES: protein motif:HMM:NF019570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HRY26383.1"
                     /translation="MPEEAVTVERLHRLETVRERAWMLLAAVERGESEHFNLSPESVS
                     MVALALASAIRETTPDLRPPLATLWPRLQRDHPAGVERLAASVAGLDGQGRARAGADM
                     ALLALLMAPDGSDHDLGALAQLFAAGGTTTRPADGPALNAPGLRRILAGDTAVTVPDE
                     VQKALRDPGPRQRLEALAAGLEQDPETFGADGRFGTLLDRLVGPAQADGLSAQALLDR
                     LAPLLDPVAGSTVRIGGSIAGDIWRHPLAWADDRSRELVPFHCLLQALVLDLLEPLAE
                     AGITLGRIEELTVPATRGLAADMLRLGLVNPRHAAVARLRHPPGSDIVVELRALSIAL
                     ADRLVDRLRQELNLTVRDLPAVRIVGPLAQLAANWRDSPAHAIAITGGCF"
     gene            6587..7216
                     /gene="upp"
                     /locus_tag="P5558_18590"
     CDS             6587..7216
                     /gene="upp"
                     /locus_tag="P5558_18590"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRY26384.1"
                     /translation="MPELIVVDHPLVQHKLTLMRRRHTATADFRRLVREVALLLGYEI
                     TRDLPLETIAIDTPLEPMQASTLAGKKLCLVSILRAGNGLLDGMLDLVPSARVGHIGL
                     YRDPATLQPVEYYYKVPEDLAERPVILVDPMLATANSAVAAVSRVKRDGATDIKFACL
                     LAAPEGVRRFHEAHPDVPIFTAAVDRGLNDHGYILPGLGDAGDRIYGTK"
     gene            7285..>8434
                     /locus_tag="P5558_18595"
     CDS             7285..>8434
                     /locus_tag="P5558_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26385.1"
                     /translation="MKPISWHANRRLSGCAFPWQLPEMEWQMRGGFAVEIVEARPGSL
                     ATLAEWLDGNGYGLTRLTPATLAGRAASPAPRVRLIEVAADQDATRLALLCRRQPDEG
                     VAVAALTDESEALSLSKALAIGVDDLLTYPFDEEELRIRLDALHQLGRARAALRERAL
                     TFARFQPPKAVSAPAAARSRRRPGGGDRPRVLVIGPASQHKVKIGEALGQAALNFADN
                     LEKAKGPILTRSFDMIVLLGTNAAARRDLLRWTDLATDHPPVTILVLDQSSDGEPLDA
                     RRRLAHLPVADRLMLPQPAELVRARMDFWLGFAALQRRLLAPPEDELRGLCHEPLTGL
                     FNHGFFLEHLRTIERGAVQRGGLVLVELINLPRLNEAAGHAAVNRYLAA"
BASE COUNT         1242 a         2820 c         3042 g         1330 t
ORIGIN      
        1 atagagcacc atgggtccgg acggcgggat caggatgccg agcgtgccgc cgccgccgac
       61 gacgccgtag gcggcgggct tcgagtagcc gtagcggatc atctggggga tggcgacggc
      121 gccgatggtg agggcggtgg cgacgctcga gcccgagatc gcggcgaaga tcgtgcaggc
      181 cgcgaccgtc gccacgccga gcccgcccgg caagtggcgg agcagggtgt gggcggtgcc
      241 gtagaggtcg tcgacgatgc ggccgcggat catgaaatgc gccatcagcg tgaagagcgg
      301 gatggcgacg aggaggtaga tgttgagctt gccgaagacg atctcgccga gccccgcgag
      361 gctgccctcg tcgaccacca tgagcgtgcc gccgaaaagg ccgagagcgg cgaagacggg
      421 caggccggtg aggatgacga ggacgaggcc ggcgagcagc agcagcagcg tcacgggcgg
      481 agtccgccgg ccccgggctc ggccggcccg gaggtgggcg actggccggt cgccatgcgg
      541 agcagcccga ccgcggccgc cacggcgagc agcaggccgc ccagcggtat gaggagctgc
      601 ggcaggtaga ccgggacggc cagatagccg gtggagtaga tgccgagcag cttcgagaag
      661 gccgcggtct gccagccgcc gatgagcagg gcgagccccg cgaccagcac ggcgagctgc
      721 ccggcggcgg cggtcagccg tcggccgcgc ggccccagcc ggtcggtcag aaggtcgacc
      781 gagatgtgct cgccgcggcg cagcgcgtcg gcggcggcga gcatgacgag gccgacgagc
      841 aggtagccca ccagctcgtc gacccagggg atcggctggt tgaggaagta gcgcatcatc
      901 acggaccagg cgacgagcgc cagcgagacg agcagcagcg ccgccgcgag gccggtgccg
      961 acgaggcaga ggcacgccac cgcccggtcg agcagcccga ccagccggcg agcgcctccg
     1021 ccgccgggcg gcgccgacgc cgtcagagct gctcgaccgc ctcgatgatg gcctcggcgt
     1081 cggggctcga tgccttgaag gcttccatca ccggcggctg catgacggcg ctgaaggccg
     1141 cggattcctc cggtgtctgg atgtgcagct tcatgccggc cttctggagc tcgtcgatcg
     1201 ccgcctccgc ggtggactcg gtgagcggga tggattccag ctcggccgca tcagccgcct
     1261 cggcgatggc ggcgcgctcg gcctcgggca ggcccgcata ccaggccggg ttgacgaaga
     1321 ggtggaagta gacggtgaag aagggtgcga ccgtgccgaa ctcctggacc tcgtagtact
     1381 tgcggctgaa ggcggccgag acgtcggtga ggccggcgtc gatcacgccg gtctggagcg
     1441 cctggtagac ctccgagccg ggcatggcgg cgggtgcggc gccggcggcg accagcccct
     1501 cgtcgaccag cttgttgagg ccgcggatct tcacgccctc gaagtcctcg acggtgatca
     1561 gcgggcgctt gctcgaggtg aagatggact ggcgggtggt gtagagccag gcgatgttct
     1621 cgaccccctt ctcgagcagc ttggcgtcga gcaggtcggc cgccttcgag cccgggaagg
     1681 cctcgatgcg ggcgagatcg gtgaagaagt aggggatggt gacgacattc atctcgggga
     1741 tggtgttgcc ccactggaag ttcaccgcga tggccccctc gatctcgccc ctggccacgg
     1801 ccgggtggtg ctgatccggc ttgtaggcct ggctggcgcc gaagatctgg acgtcgacgg
     1861 cgccatcggt gcgggcctcg acatcggcgg cgaaagcctc gacgagctgg gcgatgtgat
     1921 gcgccggcgg cagctggtgg ctgatgcgaa agaccgtctc ggcgtgcagg ctgccgagcg
     1981 gcagggtcag cgccagggcg gcgacggcaa gggtgctctt cctcttcatg acacgcgtct
     2041 ccccgatcgg ttggcttgtg gctctggtta acgcctcggc gcgcccgcgt ccatcagccg
     2101 gcgggcgaag gcgaggtcgc gctcctcggc ggcgcgcagc tcgggcaggc cgaccagcgc
     2161 gttgaaggcg gtgaaatcga gcatggcgtc ggcggcgtcg gcggtggtgc cggtcgcgtg
     2221 cagcgtctgc atcaaccggc cgatggtggc ggcgatggca taggtggtgg cgaccgggaa
     2281 ggcgaccatg gcgtagccga tcgcctcgag ctcggcggct ggcaggaacg gcgtcaggcc
     2341 gccctcgatg ttgttggcca tgcagggggc gtcgatctcg gcgcagatgc ggcgcatctg
     2401 gtcgatgtcg cgcggcgcct cgacgaagac catgtcggca ccggccgccc gatagagctg
     2461 gccccgctcg atggcgtcgt cgaggccgtt gatggcgagc gcgtcggtgc gcgccatgat
     2521 gacgaggtcc gcgtcccggc gggcatcgag ggcggccttg agcttgccga ccatctcctc
     2581 ggccggaacc acggccttgc cggccatgtg gccgcagcgc ttggggaaga gctggtcctc
     2641 gatgaagagg ccggcgaccc ctgcccgctc gtaggcgcgc accgcccggc ggacattggt
     2701 gacgttgccg aagccggtgt cggcatcggc gaagaccggc accgagacgg cgtcgcagat
     2761 gcgggcgtag tgctcggcca tctccatgag cccgagctgc gaggtgtcgg gctggccgag
     2821 cagggcagcg gtcgcggcat agccgccgca ggtgaccgcg gcgaagccgg cagcttcggc
     2881 gatcctcgcc gtcagcgcgt cctggatgcc cggcaggacg aggatcccgg gtgcgtcgac
     2941 gagacggcga aagcgggtgg tcgggcgcat ggctctgctc ctgttgcgtg gagcttatgg
     3001 ttatccgtca gcgggttgta aatggcggcc gggacgggtg cgagtcggga tgcgccggca
     3061 aaatcacgaa ggtggttgaa gttcgtcgcc tttcggcctt gtcgaatggc tgcaatatac
     3121 agattcacgt ccgttgtcag gagatgcaca tctggctcct cgatcgacag gatggctgca
     3181 tccgtcagcc cgaggcgcaa atagagctgc gtgccgatca cgtctcgggc tgggatatgc
     3241 tgctcacggg caagagagat gaactgcgct agcctggaca tcagttgatc ggtggccgga
     3301 gcggccaatc cacccttcct cagcagattg ctcgtctcgg cgagaatatg cggcgtggtg
     3361 accagcgtcg cgttacccat atggtcgagc agtagctgcc aatctcgagc gacgaagttt
     3421 tttgatcgct tgtgtctgcc aatccagcgt ttgtcgaccg cgccgatcac cagcaggagc
     3481 aagaggctcg tatcgagaag acttatcttc aatcggcaag aaccagctcg cgatgcttca
     3541 cggagaggac acgcccatcg ttgtccttga gcgttaccac cttgaaggtt ctgccgagtc
     3601 gaccgccgaa agccgcggca acccctgccg gctgatccca ggcccgcgag aatcccacgg
     3661 tgacctgcca gagcccgccg tcatcgacga actcgatctc ctctaggccg atattgctga
     3721 tgccctcgtc gtcgaacatc gtggcgacat gcgccttggc cttggcaatt gcttcgcgcg
     3781 catccgtcgc gacacccctt ctcgatagga acgccctgtg gctcgatggc tatcgagccg
     3841 tccggcattc caagccaccc gtgtccattt accgcctgaa ctgcgcccag tcgatctggc
     3901 cgtgccggtc gaggctcttc tcgatcttcg gcagcgggta tttgaggccg gtggcgcagt
     3961 tgaagaggac cgagcggtcg gtgggctgga tgcggccgtc cttcaggctc gtctgccagg
     4021 cggcgtaggt ggcggcgccc tcggggcaga ggaggaagcc ttccttgccg gcgacctcgt
     4081 cgaggccggc ctggatggca tcgtcgtcga cggcgatggc gaagccgccg ctctcgcgga
     4141 cggcgcggag gatgaggaag tcgccgacgg cctgcggcac gcgaatgccc gacgcgatgg
     4201 tggcggcgtt ctcccagcgg ggcgcgtgct cgacgccgtc ctcgtaggcc ttgaccatgg
     4261 gggcgcagcc ggtcgcctgc acggccacca tgcgcggccg cttggtgccg atgaagccga
     4321 tcgcctcgag ctcggcgaag gccttccaca tgccgatcag gccggtgccg ccgccggtcg
     4381 gatagaagat gacgtcgggc agctcccagc cgagctgctc ggcgagctcg aggcccatcg
     4441 tcttcttgcc ctcgatccgg tacggctcct tcagggtcga ggtgtcgaac cagccgaccg
     4501 tctccttgcc ggcgccgacg atcttgccgc agtcgtcgat cagcccgttg acccggtaga
     4561 cgtcggcacc ctgcagggcc atctcgctga tgttggtctc gggcgtgtcg gccgggcaga
     4621 agatggtggt cttgatgccg caatggctgg cataggccga aagggcggcg ccggcattgc
     4681 cgttggtcgg catcgccatg tgggtgatgc ccagctcctt ggccatcgcc acggccatga
     4741 cgaggccgcg cgccttgaac gagccggtcg gcaggcggcc ctcgtccttg acgatcaggc
     4801 tgccggtggc gccgtgcgtg ctcgccagcg tcggcagctc gatgagcggg gtggcgatct
     4861 cgccgaggct gacgatgttc tcggtccgcc gcaccggcag cagctcgcgc cagcgccaga
     4921 ggtccatggg ccggtcggcg agcttcgcct tggtcaacgc acccttgacg ccgtccaggt
     4981 cgtagcggac caggagcggc ttgccggcgg cggagaggtt gtgcaggcgg tcggcctcgt
     5041 agcgctcgcc ggtcatgctg cattcgagat gagtgacgaa ggtcggccgg tcctcggtga
     5101 ggttgcggtt gtcctcgatc atgcctggca tccttcccgg tcggcttgct ggatggccgt
     5161 catgaagcgg tgccgcagcg atttgtccag cctgccgtcg cgcccgacgc cgctgcagag
     5221 gtcggcgccg agcggcatgg ccattgccgc ttcttcccgc gaggcgatac aacagctctc
     5281 gacgcgcgtg cgcacgggtc tttctcgagt gcggcccctg cgtgcggccg gccggttctg
     5341 gacaggagag agcattgccc gaggaggccg tcaccgtcga gcgcctgcat cgtctggaga
     5401 ccgtgcggga gcgtgcctgg atgctgctcg ccgcggtcga gcgtggcgag agcgagcatt
     5461 tcaacctgtc gccggaatcg gtgtccatgg ttgcgctggc cctggcctcg gccatccgcg
     5521 agacgacgcc cgatctacgg ccgccgctgg cgacgctctg gccgcgcctg cagcgcgatc
     5581 atccggccgg ggtcgaacgc ctggcggcga gcgtggcggg gctcgatggt cagggccggg
     5641 ccagggcggg cgccgacatg gcgctcctgg cgttgctgat ggctccggac ggctccgatc
     5701 acgatctggg cgcgctggcg cagctcttcg ccgccggcgg cacgaccacc cgtccagccg
     5761 acggcccggc gctcaatgca cccggcctgc gccgcatcct ggccggggac acggccgtca
     5821 cggtgccaga cgaggtccag aaggctctgc gggacccggg gccgcgccag cggctcgagg
     5881 cgctggctgc cgggctcgag caggacccgg aaacgttcgg cgccgacggg cggttcggca
     5941 cgctgctcga ccgcctcgtc ggcccggcac aggccgacgg gctttcggca caggccctgc
     6001 tcgaccggct ggcgccattg ctcgatccgg tcgccggcag caccgtccgc atcggtggca
     6061 gcatcgccgg tgacatctgg cgccaccccc tcgcctgggc cgatgaccgc agccgcgagc
     6121 tggtcccgtt tcactgtctc ctgcaggcgc tcgtcctgga tctcttggag ccgctcgccg
     6181 aggccggcat cacgctcggc cggatcgagg agctgaccgt gccggcgacg agggggctgg
     6241 ccgcggacat gctgcgcctc ggcctcgtca acccgcgcca cgccgcggtg gcccggctgc
     6301 gccacccgcc cggctccgac atcgtcgtcg agctcagggc cctctcgatc gcgctggcgg
     6361 accggttggt cgatcggctc aggcaggagc tcaacctcac cgtgcgcgat ctcccggcgg
     6421 tgcgcattgt gggtcccctg gcacagttgg ccgccaactg gcgcgacagc ccggctcacg
     6481 ccattgcgat caccggcggc tgcttctagc atcctgccgg aatcctggcc gctcgcgcgg
     6541 ccgccctcat tgccacccca cgggtcgcca ccgaacggaa aggctgatgc ccgagctcat
     6601 tgtggtcgac cacccgctgg tgcagcacaa gctgacgctg atgcgtcgac gccatacggc
     6661 aaccgcggat ttccgccggc tggtgcgcga ggtggcgctg ctgctcggct acgagatcac
     6721 ccgtgacctg ccgctcgaga cgatcgccat cgacacgccg ctcgagccga tgcaggcgag
     6781 cacgctggcc ggcaagaagc tctgcctcgt ctccatcctc cgggccggca acggcctgct
     6841 cgacggcatg ctggacctcg tcccgtccgc cagggtcggg catatcggcc tctatcgcga
     6901 ccccgcgacg ctgcagccgg tcgagtacta ctacaaggtg ccggaggacc tggccgagcg
     6961 gccggtgatc ctggtcgacc cgatgctggc gaccgcgaac tcggcggtgg ccgccgtcag
     7021 ccgcgtcaag cgcgacggcg ccacggacat caagttcgcc tgcctgctcg ccgcaccgga
     7081 gggtgtgcgt cggttccacg aggcgcatcc cgatgtgccc atcttcaccg ccgcggtcga
     7141 tcgcgggctc aacgaccacg gctacatcct gcccggcctc ggcgatgccg gcgatcggat
     7201 ctatggcacc aagtagccgt tcggcggttc catggcccta ccccctgata tccgtcgcat
     7261 gatgaaggaa cggcgttaac cgagatgaaa ccgatttcgt ggcatgccaa tcgccggctc
     7321 agcggctgcg cctttccctg gcagctgccc gagatggagt ggcagatgcg gggtgggttc
     7381 gcagtcgaga tcgtcgaggc gcggccgggc agcctcgcga ccctcgcgga atggctcgat
     7441 ggcaacggct acgggctcac ccggctgaca ccggcgacgc tggccggccg ggcggcgtcg
     7501 ccggcgccgc gggtgcggct gatcgaggtc gccgccgacc aggacgcaac ccggctcgcc
     7561 ctgctctgcc gacggcagcc ggacgaaggg gtcgccgtgg cggccctcac cgacgagtcc
     7621 gaggctttgt cgttgagcaa ggcgctggcg atcggtgtcg acgacctgtt gacctatccg
     7681 ttcgacgagg aggagttgcg catccggctc gatgcgctcc accagctcgg ccgggcccgg
     7741 gcggcgctgc gcgagcgggc gctgacgttt gcccgcttcc agccgccgaa agccgttagc
     7801 gcaccggcgg ctgccaggtc gcgccggcgg ccgggcggcg gcgatcggcc gcgcgtcctg
     7861 gtgatcggcc cggcgagcca gcacaaggtc aagatcggcg aggcgctggg ccaggcagcg
     7921 ctcaacttcg ccgacaatct ggagaaggcg aaagggccga tcctcacccg cagcttcgac
     7981 atgatcgtgc ttctcggcac caatgccgca gcccggcgcg acctcttgcg ctggaccgat
     8041 ctcgcgaccg accatccgcc ggtgacgatc ctggtgctcg accagagcag cgatggcgag
     8101 ccgctcgacg ccagacggcg cctcgcccat ctgccggtgg ccgaccggtt gatgctgccg
     8161 cagccggcag agctcgtccg ggcccgcatg gatttctggc tcggcttcgc cgccctgcag
     8221 cggcggctcc tggccccgcc cgaggacgag cttcggggtc tctgccacga gccattgacc
     8281 gggctcttca atcacggctt cttcctcgag catctgcgca ccatcgagcg cggtgcggtg
     8341 cagcggggcg ggctggtgct cgtcgagctg atcaatctgc cgcggctcaa cgaggctgcc
     8401 gggcatgccg ccgtgaaccg ctatctggcc gccc
//
LOCUS       DAOTFI010000214         8385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10205_length_8385_cov_7.163745, whole genome shotgun sequence.
ACCESSION   DAOTFI010000214 DAOTFI010000000
VERSION     DAOTFI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10205_length_8385_cov_7.163745"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="P5558_18600"
     CDS             complement(<1..1210)
                     /locus_tag="P5558_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY26386.1"
                     /translation="MRRLLTALTLLAVVTATGPAVAQDAVDAAGRDVARVDPTLPTPT
                     VFDSVPRPLGSEDAALYRMIFELQEHGRWADADRLIGRLDDRLLLGHVLAQRFLHPTA
                     YVSSYPELRSWLEAYADHPEAAVIHRLAERRRPAGAEPPPEPVAGFLGGSGQELLEEA
                     ATRYDGPEGRSAKDSQAVADWLAGVRDLVARNRPTDARKLLRHPSARLADPVEQDLAR
                     WSIARSYFVNRKDQEAFELASAAAIRSGEVEPRIHWTAGLAAWRRGDITRAGRHFAAL
                     ARSDGPPGDVAAGAFWAARAALRLVEPGRYRHYLEIAAGASDGFYGILAQHVLGGPVL
                     FDWHQTAMATDHMGALMRVDGARRAMALGQLGRSAEAEAEIRKLAARASPTLTEALAA
                     LAEDRGITVDK"
     gene            complement(1195..2040)
                     /locus_tag="P5558_18605"
     CDS             complement(1195..2040)
                     /locus_tag="P5558_18605"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY26387.1"
                     /translation="MNALSALLDWYGMAGVDAAVAPGPVPAWRARAEPRDLSVPAPAR
                     PPVAAAPPATRRPVQAPDPGAVPALLVPESRRVGSARELARACESLEELEAALQAFDG
                     CALRETASRLCFADGNPQAQVMVIGEAPGAEEDREGRPFVGQSGQLLDRILRNIGLDR
                     TNVWITNVLYWRPPGNRPPTTGEIAVCLPFLERQIELLAPRFILFVGAIAAKTLLERT
                     EGVTKLRGRPFTYRTAHGTEIPCLVTFHPAYLLRQPLNKRFVWRDMLAMRARLDELGV
                     ACGAS"
     gene            complement(2268..2471)
                     /locus_tag="P5558_18610"
     CDS             complement(2268..2471)
                     /locus_tag="P5558_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26388.1"
                     /translation="MLKTSTSKNDPTARGPAQQLKTFEGKKIKPALYVGTAVGHGRYI
                     AAQDEGGKLVLDREGRPVAYRDI"
     gene            2705..4435
                     /locus_tag="P5558_18615"
     CDS             2705..4435
                     /locus_tag="P5558_18615"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY26389.1"
                     /translation="MITATLLTAVGCTDARQAASSAWPVEPALGAAEVEPAAVDSLAS
                     SYLAGRIALADGDLRIAADNLGNALDLDPDNLELRREVFALRLGVGDYPAALALAGTL
                     VEIDPDAEDAQLMLVLESVQAGDFAAAGAALPEVADRGIVGIAKPILGAWITFGAGDP
                     DAAVASLLAEERDDGLGAIRLYHAAMMLALDGRTDAARAELEPLVTSDGRAPTRLVTA
                     LVAIEAAAGQDAAAIRVLEEQLAQTPGNSVLETALAELRAGGDPPLPVTDPATGMADG
                     LLSLARALNDQNAQGQALLLARMAAYLAPGQGDVALLIAEISLAQGNPEEALRILADV
                     PADSPYGWEARLLGAQVLATMDRQDEAIARFDRMADERPERIDALVAKGDLLRRQDRF
                     AESETAYDRAIGRIDEVTVGHWPLFYARGIARERTGRWPEAEADFLHALELQPDQPQV
                     LNYLGYSWVDQGLNLEQGETMLRRAVELRPDDGYIVDSLGWAYYRLERVPESVTYLER
                     AVELQPDDPVINDHLGDAYWRAGRQREARFQWQRALTFEPEDDQVVLIERKLREGLGE
                     AGDERGANSG"
     gene            4428..5303
                     /locus_tag="P5558_18620"
     CDS             4428..5303
                     /locus_tag="P5558_18620"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRY26390.1"
                     /translation="MADEALVESARAKLNLDLLVTGRRADGYHELDSLVVFVPIADRL
                     TFEHAPGLSLEVTGPFAAALGAADDNLVLRAARALADAAGREPAFAIRLEKLLPVASG
                     IGGGSADAAATLRGLARLLGLDWPADRLRELGLDLGADVPVCLWGRPARLRGIGERLD
                     PVRGLPGLPLVLANPGIGLGTAEVFRALDPERQPVARPPFPPHPSLTRFAVWLHESRN
                     ELEPAARRLAPVIGYVLERLRREPDCLVARMSGSGATCFGLFADEESAEAAAARIARE
                     EPGWWCRAATAPGGP"
     gene            5300..6040
                     /locus_tag="P5558_18625"
     CDS             5300..6040
                     /locus_tag="P5558_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRY26391.1"
                     /translation="MILAFTDFGWRGPYLGEMRVAALRLAPSVPYVDLMADAPAFRPD
                     LAAYHLAALAGRLAPGDVVLGVVDPGVGGPRRPLALFADGVWLVGPDNGLFELVMRRA
                     GSLETHAIAWRPPALSASFHGRDLFAPTAARLALGEDAGLAPVTPIRHPGWPDEADCI
                     VHVDSYGNLVSGRRAATLPETAILQAGGRPIAHARTFSATRPGELFWYANSAGLVEIA
                     ANAASAATLLGLAPGAPLQLQATPADCT"
     gene            6052..6126
                     /locus_tag="P5558_18630"
     tRNA            6052..6126
                     /locus_tag="P5558_18630"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6084..6086,aa:Gln,seq:ttg)
     gene            6240..6656
                     /locus_tag="P5558_18635"
     CDS             6240..6656
                     /locus_tag="P5558_18635"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HRY26392.1"
                     /translation="MLSLVRTEEVAAPNGEAGYSRAEIEAMQRTVGAIFARWGISDVD
                     AAVILGGISAKTFRRWRDGEYGRVNRDLADRLSYVIGIHRALRIIFAEPAQGYGWMGR
                     PNAELDGLTPVQLLLRGGMEDLRRLRRYLDSVRGGW"
     gene            6653..7378
                     /locus_tag="P5558_18640"
     CDS             6653..7378
                     /locus_tag="P5558_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRY26393.1"
                     /translation="MTPPATRVRWPATHRLVLSHFPPIDLFDDVADPHDWEALAKAQS
                     RTNPRIHEQIGDLSLVPAERRLSGPGASWVMAAFTHLSPDRTSRFSDGSYGVYHAGDN
                     LETAMREHTFHMGRFYAAAGMAADWISEVRQLIGSIDAVLVDIRSTGFEPLLDPDSYE
                     RSQAFARKQKTANANGIVYPSVRHTGGECIAAFYPDVVTPPAQGDHFRYYWDGEQVSY
                     VRKVSGDRAIYDLRTATAASPLG"
     gene            complement(7405..>8385)
                     /locus_tag="P5558_18645"
     CDS             complement(7405..>8385)
                     /locus_tag="P5558_18645"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY26394.1"
                     /translation="PEEIVLNATTTINLRFLATAMASSLAPGDEIVVTGFDHESNIGP
                     WRGLAERGVVIRVWQLDPETLEPDLDTLDRLMTDRTRLVAVTHASNIMGGILPIRAIA
                     DVVHARGARICVDGVAYAPHRAIDVRALDVDYYAFSFYKTYGPHLAMLYGKHAALLEL
                     DGLYHYFYGKEKVPAKLEPGNPSYELAWGTTGIVDYLDELGGGTGDHAAIEKAFALIA
                     AHEAVIGGRLLDFLGSRNDVRIVGSRTADPAHRVPTISFKVDGHDSAAVVRAVDADRI
                     GIRYGDFASRRLVESLGLAPKNGVIRASMVHYNTVEEVDRLIASLERALG"
BASE COUNT         1127 a         3106 c         2929 g         1223 t
ORIGIN      
        1 gcttgtcgac ggtaatgccg cggtcttcgg ccagcgccgc cagggcctcg gtcagggtcg
       61 ggctggcccg ggccgccagc ttgcggatct ccgcctcggc ctcggcactc cggccgagct
      121 ggccgagcgc catggcccgc cgcgccccgt cgacccgcat gagagcaccc atgtgatcgg
      181 tcgccatggc cgtctggtgc cagtcgaaga gcaccggccc gccgagcaca tgctgggcca
      241 ggatgccgta gaacccgtcc gacgcaccgg cggcgatctc gagatagtgg cgatagcggc
      301 cgggctcgac caggcgaagg gcggcgcgcg ccgcccagaa ggcgccggcg gcgacgtcgc
      361 ccggcgggcc gtccgagcgg gcgagggccg cgaaatgccg cccggcccgg gtgatgtcgc
      421 cgcgccgcca ggccgcgagg ccggccgtcc agtggatgcg cggctcgacc tcgccggagc
      481 ggatggcggc ggcgctggcg agctcgaacg cctcctggtc cttgcggttg acgaaatagc
      541 tgcgggcgat ggaccagcgc gccagatcct gctcgaccgg atcggcgagc cgggccgagg
      601 ggtgccgcag gagcttgcgc gcgtcggtcg gacggttcct cgcgaccagg tcgcgcaccc
      661 ccgcgagcca gtcggcgacc gcctggctgt ccttcgccga ccggccctcc gggccgtcat
      721 agcgcgtcgc cgcctcctcc agcagctcct ggcccgagcc gccgagaaag ccggccaccg
      781 gctcgggcgg cggctccgcc ccggccggcc ggcgccgctc ggccagccgg tggatgaccg
      841 ccgcctccgg atgatcggca taggcctcga gccagctccg cagctccgga tagctggaga
      901 cataggccgt gggatggagg aagcgctggg ccaggacatg gcccagcagc aggcgatcgt
      961 cgagccggcc gatcagccgg tcggcgtccg cccagcggcc atgctcctgc agctcgaaga
     1021 tcatccgata gagggccgcg tcctcgctgc cgagcggccg cggcacgctg tcgaagaccg
     1081 tgggtgtcgg cagtgtcgga tcgacgcggg cgacgtcacg ccctgcggcg tcgaccgcat
     1141 cctgcgccac cgccggcccg gtcgccgtca cgaccgccag cagggtcagg gcagtcagga
     1201 ggcgccgcat gcgaccccca gctcgtcgag ccgtgcgcgc atggcgagca tgtcgcgcca
     1261 gacgaagcgc ttgttcaagg gttggcgcag gagataggcc ggatgaaagg tgacgaggca
     1321 gggaatctcg gtgccgtggg ccgtgcggta ggtgaagggg cggccgcgca gcttggtgac
     1381 accctcggtg cgctccagca gcgtcttcgc ggcgatggcg ccgacgaaga ggatgaagcg
     1441 cggtgccagg agctcgatct gccgctcgag gaacggcagg cacacggcga tctcgccggt
     1501 cgtcggcggc cgattgccgg gtggccgcca gtagaggacg ttggtgatcc agacgttggt
     1561 gcggtcgagg ccgatattcc ggaggatccg gtccagaagc tggccgctct ggccgacgaa
     1621 ggggcgcccc tcgcggtcct cctcggcgcc cggcgcctcg ccgatcacca tcacctgcgc
     1681 ttgcgggttg ccatcggcga agcagaggcg gctcgcggtc tcgcggagcg cgcagccatc
     1741 gaaggcctgc agggccgcct cgagctcctc gaggctctcg caggcccggg cgagctcacg
     1801 ggcgctgccg acgcgccggc tctccggcac gagcagcgcc ggcacggcgc cggggtcggg
     1861 cgcctggacg gggcggcggg tcgcaggcgg ggctgcggcg accgggggcc tcgccggcgc
     1921 cggcacggac aggtcgcggg gctcggctcg agcgcgccag gctggcaccg gcccgggcgc
     1981 cacggcagcg tcgacgccgg ccatgccata ccagtcgagc agcgccgaca gcgcattcat
     2041 atccgtcggc cgagctggcg tttgtccgcc tggtctcatg tccctccgac ccgggccgct
     2101 ggcccacgcg atcttccacg gccgcccgcg cgaccgccga atcgtcttct cgcccggccc
     2161 tccccggatg cgggggaggc cgttcgtcag gcagatccac ccctgcccag ggcgagcctc
     2221 ctggcgatcg aggcgtcacg ccgtgccagg gcgtgacgcg aaaaacgcta gatgtcgcga
     2281 taggcgaccg gccggccttc gcggtccagc accagcttgc cgccctcatc ctgggccgct
     2341 atgtagcggc catgaccgac ggcggtgccg acatagaggg ccggcttgat cttcttgccc
     2401 tcgaacgtct tcagctgctg cgccggtcca cgggccgttg ggtcgttctt gctcgtcgac
     2461 gtcttgagca tcgctcacgc tccgcaatat ctatgatgcc acgttgtcgc tgagcagcgt
     2521 ggccatgttg tccggtttgt ggcttgggcg gcgacgatcg tcaagtcggt tcaccggact
     2581 cgatgccgcg ccgcggccgg cccggtgttc cgtcattggc cgccggcgag gccggcgtgc
     2641 atttttggag catggaatga gctcagggcc tgcggcgaga agcggacctc cggtgctggc
     2701 cgtcatgatc acggccaccc tgctcacggc cgtgggctgc accgatgcgc gccaggcggc
     2761 gagcagcgcc tggccggtcg agcccgccct cggcgctgcc gaggtcgagc cggcagcggt
     2821 cgacagcctc gcctcgagct atctggccgg ccgcatcgcg ctcgccgacg gcgacctgcg
     2881 cattgccgcc gacaatctgg gcaacgccct cgacctcgac cccgacaatc tcgagctccg
     2941 gcgcgaggtc ttcgcgctgc gcctcggcgt cggcgactac ccggcggcgc tggcgctcgc
     3001 cggaacgctg gtcgagatcg acccggacgc cgaggacgcc cagctcatgc tcgtcctcga
     3061 gagcgtgcag gccggcgact tcgccgccgc cggggcggca ttgcccgagg tcgccgatcg
     3121 cggcatcgtc ggcatcgcca agccgatcct cggcgcctgg atcaccttcg gcgccggcga
     3181 tccggatgcg gccgtggcca gcctcctcgc ggaggaacgc gacgacggcc tcggcgccat
     3241 ccgcctctac cacgcagcga tgatgctcgc cctcgatggc cggaccgacg ccgcccgcgc
     3301 ggagctcgag ccgctcgtca cgagcgatgg ccgggccccg acccggctcg tcacggccct
     3361 ggtcgccatc gaggcggcgg ccggccagga cgcggccgcg atccgggtgc tcgaggagca
     3421 gctcgcccag acgccgggca acagcgtgct cgagacggcg ctggccgagc ttcgggccgg
     3481 cggcgacccg ccgctgccgg tgaccgaccc cgccaccggc atggcggacg ggctgctcag
     3541 cctcgcccgg gcgctcaacg accagaatgc gcaaggccag gcgctgctgc tggcgcgcat
     3601 ggcggcctat ctggcaccgg gtcaggggga cgtggccctg ctcatcgccg agatctcgct
     3661 cgcccagggc aacccggagg aggcgctgcg catcctcgcc gacgtgcctg ccgattcgcc
     3721 ctatggctgg gaggcccgcc tcctcggcgc ccaggtcctg gcgaccatgg accggcagga
     3781 cgaggcgatc gcccgcttcg accggatggc cgacgagcgg cccgagcgga tcgacgcgct
     3841 ggtcgccaag ggcgacctgt tgcggcggca ggaccgcttc gccgagtccg agacggccta
     3901 cgaccgggcg atcggccgga tcgacgaggt cacggtcggc cactggccgc tcttctacgc
     3961 gcgcggcatc gcccgcgagc gcaccgggcg ctggccggag gccgaggccg acttcctcca
     4021 tgccctcgag ctgcagcccg accagccgca ggtgctcaac tatctcggtt actcgtgggt
     4081 cgaccagggc ctcaatctcg agcagggcga gacgatgctg cgccgggcgg tcgagctgcg
     4141 gcccgacgac ggctacatcg tcgacagcct cggctgggcc tattatcgcc tggagcgggt
     4201 gcccgaatcg gtgacctatc tcgagcgcgc ggtcgagctg cagcccgacg acccggtcat
     4261 caacgaccat ctgggcgatg cctactggcg cgccggtcgc cagcgcgagg cccgcttcca
     4321 gtggcagcgc gccctgacct tcgagcccga ggacgaccag gtcgtgctca tcgagcgcaa
     4381 gctgcgcgag gggctgggcg aggccgggga cgagcgcggg gcgaatagtg gctgatgagg
     4441 ctctggtcga gtccgcccgt gccaagctga acctcgacct cctcgtcacc ggccgccgtg
     4501 ccgacggcta ccacgagctc gacagcctcg tcgtcttcgt gccgatcgcc gaccggctca
     4561 ccttcgagca tgctcccggg ctctcgctcg aggtcaccgg ccccttcgcc gccgccctcg
     4621 gtgcggccga cgacaatctc gtcctgcgcg ccgcacgcgc gctcgccgac gccgccggcc
     4681 gggagcccgc tttcgccatc cgcctggaga agctgctgcc ggtggcgtca ggcatcggcg
     4741 gcggctcggc cgacgcggcg gcgacgctgc gcggcctcgc ccgcctcctc ggcctcgact
     4801 ggccggccga ccgcctgcgc gagctgggcc tcgacctcgg cgccgatgtc ccggtctgcc
     4861 tctgggggcg acccgcccgg ctgcgcggca tcggcgagcg gctcgacccg gtgcgcggcc
     4921 tgcccggcct gcccctcgtc ctggccaatc ccggcatcgg cctcggcacg gccgaggtgt
     4981 tcagggcgct cgacccggag cgccagcccg tcgcccggcc gcccttcccg ccgcacccgt
     5041 cgctcacccg cttcgccgtc tggctgcacg aaagccgcaa cgagctcgag cccgccgccc
     5101 ggcgcctggc gccggtgatc ggctacgtgc tcgagcggct gcgcagagag cccgactgcc
     5161 tggtcgcccg gatgtcgggc tcgggggcca cctgcttcgg cctcttcgcc gatgaagaga
     5221 gcgctgaggc cgccgccgcc cggatcgccc gggaggagcc cggctggtgg tgccgcgccg
     5281 ccaccgcccc gggcgggcca tgatcctcgc cttcaccgat ttcggctggc ggggccccta
     5341 tctcggcgag atgcgcgtgg cggccctgcg tctcgcccct tccgtccctt acgtcgacct
     5401 catggcggac gcacccgcct tccggccgga cctcgccgcc taccacctcg ccgccctggc
     5461 aggccggctc gcacccggcg acgtcgtcct cggcgtcgtc gacccgggtg tgggcggccc
     5521 gcgccggccg ctggcgctct tcgccgacgg cgtctggctc gtcggccccg acaacgggct
     5581 tttcgagctc gtcatgcgcc gcgccggcag cctcgagacg cacgcgatcg cctggcggcc
     5641 gcccgccctc tcggcgagct tccacggccg cgacctcttc gccccgaccg ccgcccgcct
     5701 cgctctgggc gaggacgcgg ggctcgcacc cgtgacgccg atccgccacc cgggctggcc
     5761 ggacgaggcc gattgcatcg tccatgtcga ctcttacggc aatctcgtca gcggccgccg
     5821 ggcggcgacc ctgcccgaga cggccatcct ccaggccggc ggccgaccca tcgcccatgc
     5881 ccggaccttc agcgctaccc gcccgggcga gctcttctgg tacgccaact cggccggcct
     5941 ggtcgagatc gccgccaatg ccgcctcggc cgccaccctc ctcggcctcg cccccggagc
     6001 accgctccag ttgcaagcca cccccgccga ctgtacatga acgctggcgc ttggggcgtc
     6061 gccaagtggt aaggcaccgg gctttggtcc cggcatccgc aggttcgaat cctgccgccc
     6121 cagccagcct ccctgacttc ccgttatttt cgtgtcctcg ccggcgaccg cagccttttg
     6181 gcctgcctat ggacaattag gcgcttatgt cctatttttg gacaaaaggg gatgacacga
     6241 tgctctctct cgtccgaacg gaagaagtcg cggcgccaaa tggcgaggcc ggctattcgc
     6301 gagccgagat cgaggcgatg cagcggaccg tgggcgcgat cttcgcccgc tgggggatca
     6361 gcgacgtcga cgcggcggtg atcctgggcg gcatctcggc gaagaccttc cggcgctggc
     6421 gcgacggcga gtacggccgg gtcaaccgag acctcgccga ccgcctgtcc tatgtcatcg
     6481 gcatccaccg ggcgctccgg atcatcttcg ccgagccggc gcagggctat ggctggatgg
     6541 gccggcccaa tgcggagctc gatggcctga cgcccgtgca gcttctgctg cgcggcggca
     6601 tggaggatct gcgccggttg cgccgctatc tggattcggt gcggggcggc tggtgacacc
     6661 gccggccacc cgcgtccgat ggccggccac gcaccgcctc gtgctctcgc atttcccgcc
     6721 gatcgacctc ttcgacgacg tcgccgaccc gcacgactgg gaagccctgg cgaaggccca
     6781 gtcgcgcacc aacccgcgca tccacgagca gatcggcgat ctctcgctgg ttccggcgga
     6841 gcgccgcctc tcgggtcccg gcgcttcctg ggtgatggct gccttcaccc acctctcgcc
     6901 agaccgcacc tcgcgcttca gcgacggcag ctacggcgtc taccatgccg gcgacaacct
     6961 cgagaccgcc atgcgcgagc acacctttca catggggcgg ttctatgccg ccgccggcat
     7021 ggcagccgac tggatctccg aggtccgcca gctgatcggc tcgatcgacg ccgtgctcgt
     7081 ggatatccgc agcaccggct tcgagcccct gctcgacccg gattcctacg agcgctcgca
     7141 agccttcgcc cggaagcaaa aaacggcaaa cgccaatggc atcgtctacc ccagcgtgcg
     7201 ccacaccggc ggtgaatgca tcgccgcctt ttatccggac gtcgtgacgc cgcccgccca
     7261 gggcgaccac tttcgctact actgggacgg cgagcaggtc agctatgtcc gcaaggtcag
     7321 cggcgaccgg gcgatctacg acctgcggac ggccactgcg gcatcgccgc tgggctgagt
     7381 agggagccca gccagccggc cggctcagcc cagtgcccgc tctaggctcg cgatcagccg
     7441 gtcgacctcc tccaccgtgt tgtagtggac catcgaggcc cggatgacgc cgtttttcgg
     7501 cgccaggccc aggctctcga ccagccgcct cgaggcgaag tcgccgtagc ggatgccgat
     7561 ccggtcggca tcgacggcgc gcaccaccgc cgccgaatcg tggccgtcca ccttgaacga
     7621 gatagtcggc acgcggtgtg ccggatcggc ggtgcggctg ccgacgatgc gcacgtcgtt
     7681 gcggctgccg aggaaatcga gcagccggcc gccgatcacc gcctcgtgcg cggcgatcag
     7741 cgcgaaagcc ttttcgatcg ccgcgtggtc gccggtgccg ccgccgagct cgtccagata
     7801 gtcgacgatg ccggtggtgc cccaggccag ctcgtagctg ggattgccgg gctcgagctt
     7861 ggccgggacc ttctccttgc catagaagta gtgatagagc ccgtcgagct cgagcagcgc
     7921 cgcatgcttg ccgtagagca tggccagatg cgggccgtag gtcttgtaga agctgaacgc
     7981 atagtaatcg acgtcgagtg cgcgcacatc gatggcccga tgcggggcgt aggcgacccc
     8041 gtcgacacag attctcgcac cgcgcgcatg gacgacgtcg gcgatggcgc ggatcggcag
     8101 gatgccgccc atgatgttcg aggcgtgggt cacggcgacc agccgggtgc ggtcggtcat
     8161 cagccggtcg agcgtgtcga ggtcgggctc cagggtctcg ggatcgagct gccagacgcg
     8221 gatcacgacg ccgcgctcgg caagcccgcg ccaggggccg atgttcgatt cgtggtcgaa
     8281 gccggtcacc acgatctcgt cgcccggggc gagggagctc gccatggcgg tcgcgaggaa
     8341 gcgcaggttg atcgtggtcg tggcgttgag cacgatctcc tccgg
//
LOCUS       DAOTFI010000215         8339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10288_length_8339_cov_7.573515, whole genome shotgun sequence.
ACCESSION   DAOTFI010000215 DAOTFI010000000
VERSION     DAOTFI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10288_length_8339_cov_7.573515"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="P5558_18650"
     CDS             <1..354
                     /locus_tag="P5558_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRY26395.1"
                     /translation="ELTDEEKEYKSKRKLVQEKLIKFATRIPAFMYLTDFRENTLQDV
                     ITKLEPDLFHAVTGLTVADFHLLVRLKVFNTERMNEAVFAFRRYEDASLRYTGIESHE
                     GLTHYGLYDTVVARE"
     gene            369..656
                     /locus_tag="P5558_18655"
     CDS             369..656
                     /locus_tag="P5558_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26396.1"
                     /translation="MRPKVIGAGVATGPDHVFIALRGRYLGGNQQKIRWGAGRIILTA
                     FLSAKNSLKFYETQDQLPDKIETILAPLGHFTVDDRVSLFYKQFRVHGFLL"
     gene            complement(1007..2029)
                     /locus_tag="P5558_18660"
     CDS             complement(1007..2029)
                     /locus_tag="P5558_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRY26397.1"
                     /translation="MSDAASRISPTIDLDAPGKRQGHLHVPWSRDRSAWGAVRPPITV
                     LRGGQGPHLLLLAGNHGDEYEGIIALLKLAETLGAENLAGAVTILPSLNHPAVLAGSR
                     ASPIDQGNMNRVFPGRRVGTVTEMIADFVLRELVARADVVVDLHAGGKSLDFVPSVVL
                     HDLPVPDHMQRTMEAARAFGAPLTLLLDELDMEGMLDLHVERLGKLFLSTELGGGGGT
                     TPERVAIAERGLQNLLVHFGLVPGRLDLPRPSRLMAAPNAGFVVSDTAGILEPLVPLG
                     AEVRSGTPLARIHAIEDTGVPARTFTAPMDGLLYARHHPGLIGRGDCLAVLGRDTTRP
                     GHDPDG"
     gene            complement(2049..2822)
                     /locus_tag="P5558_18665"
     CDS             complement(2049..2822)
                     /locus_tag="P5558_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRY26398.1"
                     /translation="MTPDELLDIAIAVSEAARPVPLRYFRQPLEIIAKADDSPVTIAD
                     RETEAVMRALLAERVPGHGILGEEHGQERLDHRFVWVLDPIDGTRSFITGFPLFNNLI
                     AVLDQGRPVVGVVDTPAIAERYHAARGRGAWFNGQPIRTSGCTELANAAVYLAGHAPA
                     DPAEAARFQRLQHKGRLQRQGYDAYVYALLAAGHLDLMIETDLQPYDHLALVTLIEEA
                     GGAITDWAGNPLGLDSKGDVLAAATPALHAEALTLLAPA"
     gene            complement(2841..3236)
                     /locus_tag="P5558_18670"
     CDS             complement(2841..3236)
                     /locus_tag="P5558_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRY26399.1"
                     /translation="MILVDSSVWIDFFRGLDTSGALLLREILRQDDVLAGDLVLVEVL
                     QGFQDEREAAVAADLLGRFDQVAIVDIRIAKEAAAHYRRLRRLGVTVRKTIALLIATR
                     CIDKGWWLLHNDRDYEPLREHLGLRVLGD"
     gene            complement(3233..3439)
                     /locus_tag="P5558_18675"
     CDS             complement(3233..3439)
                     /locus_tag="P5558_18675"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRY26400.1"
                     /translation="MRTNIDIDEKLIEEARRITGLASKKAIVEAALEELVRRARQREV
                     ADYFGKLPSWEGNLDESRKGRAFD"
     gene            3591..4376
                     /locus_tag="P5558_18680"
     CDS             3591..4376
                     /locus_tag="P5558_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26401.1"
                     /translation="MTQAAPTETAALLAENVHKRFGNFEVLKGISLTARAEDVISIIG
                     ASGSGKSTFLRCINFLETPDSGRIVVGGEEIRVRAGRRGLPVVSDQAALGRIRARLGM
                     VFQGFNLWSHMTVLQNVMEGPVTVLREPKGEVRDRAMDLLAKVGIAEKHDAYPVELSG
                     GQQQRVAIARALCMQPQVLLFDEPTSALDPELVGEVLKVMRDLAAEGRTMVVVTHEMG
                     FAREVSSRVMFLADGRVEEESSPAELFGNPRSERCRRFLASVL"
     gene            4395..5234
                     /locus_tag="P5558_18685"
     CDS             4395..5234
                     /locus_tag="P5558_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRY26402.1"
                     /translation="MKPSPLLALVAGVLFAGSAAAQDWSTIRIATEGAYAPWNFTNAA
                     GQLDGFDVDLANELCARMGAQCEIVAQAWDGIIPALQAGRYDAIMAGMSITDARMEVI
                     DFTRSYATTPAIFVAPKDSDFAAAGLPETRVDMTEISAEEQALLDQVKGLLDGLTVGV
                     QTATIHENFLREHMGDIDIRTYDSQENLDLDLQAGRIDVALADQSGWQPLLEGELGED
                     FTRVGPGFARGIFGRGVGVGIRKEDAALKDKFNAAIQSALDDGTVSELAIKWFGFDSA
                     TTD"
     gene            5256..5966
                     /locus_tag="P5558_18690"
     CDS             5256..5966
                     /locus_tag="P5558_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26403.1"
                     /translation="MDLTLLAWGDAGWGDQMALGALVTLAVAICAYALGIVVGAGLAA
                     MKLSGLPPLRWIAQAYTTVIRGIPELLVIYLVFFGGGAALRAIASGMFGYEGYVDLPI
                     FLTGTICIGASAGAYSAEVIRGAVLVVPKGQIEAAIAVGMTRRQRFWRVLVPQVARYA
                     LPGLGNIWQFTLKDTSLISVIGLVEIMRQAAIAAGSTKEPFTFYVVAALLYLGLTSLS
                     NRGFLRAESWANRGVRRA"
     gene            5994..6728
                     /locus_tag="P5558_18695"
     CDS             5994..6728
                     /locus_tag="P5558_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26404.1"
                     /translation="MDWAQLADVATRLAGGLPTTLLLVGQSLLIGFVLAVLVALMRVS
                     PNPLLSAPAYGFVYVFRSTPLLVQLFLIYYGSGQFRPFFQDVGLWFLFRDAWFCAILA
                     LTLNTGAYTSEIIRGGIQSVPHGQLEAGRAVGMSPAQLFRRITFPVAIRQALPAYGNE
                     VILMVKASSLASTITILEITGIAKTIIAQTFRPVEVFIVAGALYLAINFVATQAILYA
                     ERRLSPASAADRAKAAERRQKAMLAR"
     gene            complement(6750..>8339)
                     /locus_tag="P5558_18700"
     CDS             complement(6750..>8339)
                     /locus_tag="P5558_18700"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY26405.1"
                     /translation="LARRLAEAGCRFAFGIPGGEILTLIDALAAAGITFVTTKHETAA
                     GFMAEAVWQRTGTPPVLVATVGPGISNAVNVVANARLDRVPLIVLVGAVDADEQLAYN
                     HQIIDHKKLLEPAIKGAFTLTATAADLVTERALALAGDPRPGPVLIEVPISVADAPAA
                     ATTPIRRAPTAPVAPAAGPVLDEARTWLAEARRPLVIAGFDAVQHDAGAALARFALTQ
                     RAPVIQTYKAKGLLPEDHDFALAAAALSPVADRQLEAEIEKADLVVLAGYDAVEMRRD
                     WQHPWDTTQKRVIAFDAVANDHYLHQAGIGFVCDIGAGLDALGQEVRSASTWTPAAVG
                     AVRDQVLALFRSDEAWGPAAICEVARRVLPRDTVAAADVGAHRILLSQVWPAYAPRTL
                     LHSNGLCTMGCGLPLAIGAKIAEPDRPVVVFTGDGGLLMVLGELATAVEQGLPIIVIC
                     FVDHSLALIEVKQRERQLTRQGVAVGAFDAVQLAAGLGGHGVVAADRKSLAAALEAAL
                     GADCFTLIACPIAEDAYDGRI"
BASE COUNT         1217 a         2706 c         3057 g         1359 t
ORIGIN      
        1 gagctgacgg acgaggaaaa ggaatacaaa tctaaacgca agctcgtgca ggaaaagctc
       61 atcaaatttg caacgcgcat tcctgccttc atgtacctga cggatttccg cgagaacacg
      121 ctccaggacg ttatcaccaa gctggaaccg gacctgttcc atgccgtcac cggcttgacg
      181 gtggcagact tccacctgct ggtacgcctc aaggtgttca atacggagcg gatgaacgaa
      241 gccgtctttg cctttcgccg gtatgaggat gcctcgctgc gctacacggg catcgaaagc
      301 cacgaggggc tgactcacta cggcctgtac gacaccgtcg tagcccgaga gtagcggcga
      361 gttgcttcat gcgtcctaaa gtgatcgggg ccggtgtcgc caccggcccc gatcacgtgt
      421 tcatcgcgct ccgcggccgt tacctcggcg gcaaccaaca aaaaatccgg tggggtgcgg
      481 ggaggatcat cctcaccgcc ttcttgtctg ctaaaaactc cctaaaattt tacgaaactc
      541 aagatcaatt accggacaaa atcgaaacga tacttgctcc gttgggtcac tttacagttg
      601 atgacagggt gtcgctcttc tataaacagt ttcgtgttca tgggtttctc ctgtgacacc
      661 cgacttgagg cgtttcctcc ctattgagct gggccgcggc gagagccgcg gccctctttt
      721 tttgtcctgc ggggtggggc ggggtgcggg tggcggggat ttcgctaaaa ttttaggcga
      781 atgaagataa acatcagaat aaaatcgaaa cgatacttgc gattcttggt gagttattgg
      841 ttgatgccgg ggggctgctc aactataaac agtctcgtgt tcatgggttt ctcctgtgac
      901 acccgacttg aggcgtttcc tccctattga gctgggccgc ggtgagaacc gcggccctct
      961 ttttttgcgc gaatccggtc ttgcgcgggg cggggcgggc tgggcttcag ccgtccgggt
     1021 cgtgtcccgg gcgggtcgtg tcccggccga ggacggcgag gcagtcgccg cggccgatca
     1081 ggcccgggtg gtggcgggcg tagaggaggc cgtccatggg ggccgtgaat gttcgggcgg
     1141 gcacgccggt gtcctcgatg gcgtggatgc gggcgagggg ggtgccgctt cgcacctcgg
     1201 cgccgagcgg cacgagcggc tcgaggatgc cggcggtgtc gctgacgacg aagcctgcgt
     1261 tcggggcggc catgaggcgg ctcggccggg gcaggtcgag ccggcccggg acgaggccga
     1321 aatggacgag gaggttttgc aggccgcgct cggcgatggc gacgcgctcg ggcgtggtgc
     1381 cgccgccgcc gccgagctcg gtcgagagga agagcttgcc caggcgctcg acgtggaggt
     1441 cgagcatgcc ttccatgtcg agctcgtcga ggaggagggt gaggggggcg ccgaaggccc
     1501 gggcggcttc catggtgcgc tgcatgtggt ccggcacggg gaggtcgtgg aggacgacgc
     1561 tcggcacgaa gtcgaggctc ttgccgccgg cgtggaggtc gacgacgacg tcggcgcggg
     1621 cgaccagctc gcggagcacg aagtcggcga tcatctcggt gaccgtgccg acgcgtcggc
     1681 ccgggaagac gcggttcatg ttgccctggt cgatgggcga ggcgcggctg ccggcgagca
     1741 cggccgggtg gttgagggag gggaggatgg tgacggcgcc ggcgaggttt tcggcgccga
     1801 gcgtttcggc gagcttgagg agcgcgatga tgccctcgta ctcgtcgccg tgattgccgg
     1861 cgaggaggag gaggtggggc ccctggccgc cgcgcagcac ggtgatcggg gggcggacgg
     1921 cgccccaggc ggagcggtcg cggctccagg gcacgtgcag gtggccctgg cgcttgcccg
     1981 gggcgtcgag gtcgatggtg gggctgatgc gcgaggcggc gtcgctcacg tccgttgtcc
     2041 tggcgtcctc aggcgggggc gaggagcgtg agcgcctcgg cgtggagcgc gggggtggcg
     2101 gcggcgagca cgtcgccttt cgagtcgagg ccgagcgggt tgccggccca gtcggtgatg
     2161 gcgccgccgg cttcctcgat cagggtgacc agggccaggt ggtcgtaggg ctggaggtcg
     2221 gtctcgatca tgaggtcgag gtggccggcg gcgaggaggg cgtagacata ggcgtcgtag
     2281 ccctggcgct ggaggcggcc cttgtgctgc aggcgctgga agcgggcggc ctcggcgggg
     2341 tcggcggggg cgtggccggc gaggtagacg gcggcgttgg cgagctcggt gcagccgctg
     2401 gtgcggatgg gctggccgtt gaaccaggcg ccccggccgc gggctgcgtg gtagcgctcg
     2461 gcgatggccg gggtgtcgac gacgccgacc accgggcggc cctggtcgag gacggcgatg
     2521 aggttgttga agagcgggaa gccggtgatg aaggacctgg tgccgtcgat cgggtcgagg
     2581 acccagacga agcggtggtc gaggcgttcc tggccgtgct cctcgccgag gatgccgtgg
     2641 ccggggacgc gctcggcgag gagggcgcgc atgacggcct cggtctcgcg gtcggcgatg
     2701 gtgacgggcg agtcgtcggc cttggcgatg atctcgaggg gctggcggaa gtagcggagc
     2761 gggaccgggc gggccgcctc gctgacggct atggcgatgt cgaggagctc gtccggggtc
     2821 atggcgctca ccgtgtttgt tcagtcgccg agcacgcgca ggccgagatg ctcgcgcagc
     2881 ggctcgtagt cgcggtcgtt gtgcagcagc caccagccct tgtcgatgca gcgcgtggcg
     2941 atcagcaggg cgatggtctt gcggacggtg acgccgagcc ggcggagccg gcgatagtgg
     3001 gcggcggcct ccttggcgat ccggatgtcg acgatcgcca cctggtcgaa gcgaccgagg
     3061 agatcggccg ccaccgccgc ctcgcgctcg tcctggaagc cctgcagcac ctcgaccagg
     3121 acgaggtcgc ccgccagaac gtcgtcctgc cgcaggatct cccggagcag cagggcgccg
     3181 gacgtgtcga gcccgcgaaa gaaatcgatc cagacgctgc tgtcgacgag gatcaatcga
     3241 aggcccgccc cttgcgggac tcgtccaggt tgccctccca ggagggcagc ttgccgaaat
     3301 agtcggccac ctcccgctgt cgggcccgcc taaccagctc ttccagcgcc gcctcgacga
     3361 tcgccttctt gctggcgagg ccggtgatgc gccgggcctc ctcgatcagc ttctcgtcga
     3421 tgtcgatatt ggtgcgcatg tcatgacccg atgtgcagaa tgatgacact tcttacacca
     3481 tcggcgtgca ggtctcaatt ggtgtcgagc ggcggcgggc cccaccgcgg gcttgccgcg
     3541 tcggccgtcc ctctctaccg tcgcagcggc aaccgcgagc gccgcacgac atgacccagg
     3601 ccgccccgac cgagaccgcc gccctgctgg cggagaacgt ccacaagcgc ttcggcaatt
     3661 tcgaggtgct gaagggcatc tcgctgaccg cgcgggccga ggacgtcatc tcgatcatcg
     3721 gcgcctcggg ctcgggcaag agcacgttcc tgcgctgcat caacttcctc gagacgccgg
     3781 acagcggccg gatcgtcgtc ggcggcgagg agatccgggt ccgcgccggc cggcgcggcc
     3841 tgcccgtggt cagcgaccag gcggcgctcg gccggatccg cgcccggctc ggcatggtgt
     3901 tccagggctt caatctctgg tcgcacatga ccgttttgca gaatgtgatg gagggcccgg
     3961 tcacggtgct gcgcgagccc aaaggcgagg tcagggaccg ggccatggat ctcctcgcca
     4021 aggtcggcat cgccgagaag cacgacgcct atccggtcga gctctccggc ggccagcagc
     4081 agcgggtggc cattgcccgt gccttgtgca tgcagccgca ggtgctgctc ttcgacgagc
     4141 cgacctcggc cctcgacccg gagctggtcg gcgaggtcct gaaggtcatg cgcgacctcg
     4201 ccgccgaggg ccgcacgatg gtcgtcgtca cccacgagat gggcttcgcc cgcgaggtct
     4261 cgagccgggt gatgttcctg gccgatggcc gggtcgagga ggagagctcc ccggccgagc
     4321 tcttcggcaa cccgcgctcc gagcgctgcc ggcgctttct tgccagtgtc ctctagatcc
     4381 ggagaatcca accgatgaag ccctcgccat tgctcgccct cgtcgccggt gtgctcttcg
     4441 ccggcagtgc cgcggcccag gactggagca ccatccgcat cgccaccgag ggcgcctacg
     4501 cgccctggaa cttcaccaac gcggcgggcc agctcgatgg cttcgatgtc gatctggcga
     4561 acgagctctg cgcccgcatg ggggcccagt gcgagatcgt cgcccaggcc tgggacggca
     4621 tcatccccgc cctccaggcc ggccgctacg acgcgatcat ggcgggcatg tcgatcaccg
     4681 acgcgcgcat ggaggtgatc gacttcacgc gctcctacgc gacgacgccg gcgatcttcg
     4741 tggcgccgaa ggacagcgac ttcgccgctg ccggcctgcc cgagacccgc gtcgacatga
     4801 cggagatctc ggccgaggag caggcgctgc tcgaccaggt gaaggggctg ctcgacggcc
     4861 tgacggtcgg cgtgcagacc gcgaccatcc acgagaactt cctgcgcgag cacatgggcg
     4921 acatcgacat ccgcacctat gacagccagg agaacctcga cctggacctg caggccgggc
     4981 gcatcgacgt ggcgctggcc gaccagagcg gctggcagcc cttgctcgag ggcgagctcg
     5041 gcgaggactt cacgcgggtc ggcccgggct tcgcccgcgg catcttcggc cgcggcgtgg
     5101 gtgtcggcat ccgcaaggag gacgcggcgc tgaaggacaa attcaatgcc gccatccagt
     5161 cggcgctgga cgacggcacg gtgagcgagc tggcgatcaa gtggttcggc ttcgacagcg
     5221 ccaccacgga ctgagcggcg agcgggccgg ggctcgtgga cctgacgctc ctcgcctggg
     5281 gggacgccgg ctggggcgac cagatggcgc tgggcgccct cgtcaccctg gccgtcgcca
     5341 tctgcgccta tgcgctcggc atcgtcgtcg gcgccggcct cgccgcgatg aagctctcgg
     5401 gcctgccgcc gctccgctgg atcgcccagg cctacacgac ggtcatccgc ggcattcccg
     5461 agcttctcgt gatctacctc gtcttcttcg gcggcggggc ggcgcttcgg gccatcgcca
     5521 gcggcatgtt cggctacgag ggctatgtcg acctgccgat cttcctcacc ggcacgatat
     5581 gcatcggcgc cagcgccggc gcctactcgg ccgaggtgat ccggggcgcc gtgctggtcg
     5641 tgccgaaggg ccagatcgag gcggcgatcg ccgtcggcat gaccaggcgg cagcgcttct
     5701 ggcgggtgct ggtgccgcag gtcgcacgct acgcgctgcc gggcctcggc aatatctggc
     5761 agttcacgct caaggacacg tccttgatct cggtgatcgg gctcgtcgag atcatgcgcc
     5821 aggcggcgat cgcggcgggc tcgaccaagg agcccttcac cttctacgtc gtcgccgccc
     5881 tgctctatct gggcctcacc tcgctctcca atcgcggctt cctcagggcc gagagctggg
     5941 ccaatcgagg cgtcaggcgc gcctgatggc cgagatttga tggccgacac ttgatggact
     6001 gggcccagct cgccgatgtc gcgacccgcc tcgccggcgg cctgccgacg acgctgctgc
     6061 tggtcggcca gtcgctgctc atcggcttcg tgctggccgt gctcgtggcg ctgatgcggg
     6121 tgagcccgaa cccgctcctg agcgccccag cctacggctt cgtctatgtc tttcgcagca
     6181 cgccgctgct cgtccagctc ttcctgatct attacggctc gggccagttc cgcccgttct
     6241 tccaggatgt cgggctctgg ttcctcttcc gcgatgcctg gttctgcgcc atcctggcgc
     6301 tgaccctcaa caccggcgcc tacacgagcg agatcatccg gggcggcatc cagtcggtgc
     6361 cgcacggcca gctcgaggcc ggccgggccg tcggcatgag cccggcccag ctctttcgcc
     6421 gcatcacctt ccccgtcgcc atccgccagg ccctgccggc ctatggcaac gaggtgatct
     6481 tgatggtcaa ggcgagctcg ctggcctcga ccatcaccat cctcgagatc accggcatcg
     6541 ccaagacgat catcgcccag accttccgcc cggtggaggt cttcatcgtc gccggtgcgc
     6601 tctatctcgc catcaacttc gtcgccaccc aggcgatcct ctacgccgag cgacggctgt
     6661 cgccggcctc ggccgccgac cgtgccaagg cggccgagcg ccgccagaag gcgatgctgg
     6721 cgcgctgaac gacaacgatc cgggtcgggt cagatccgcc cgtcataggc gtcctcggcg
     6781 atcgggcagg cgatgagggt gaagcagtcg gcgccgagcg ccgcttcgag ggcggcggcg
     6841 aggcttttcc ggtcggcggc caccacgccg tggccgccga ggcccgccgc gagctggacg
     6901 gcgtcgaagg cgcccacggc gacgccctgc cgggtgagct ggcgctcgcg ctgcttgacc
     6961 tcgatcaggg cgagcgagtg gtcgacgaag cagatgacga tgatgggcag gccctgctcg
     7021 acggcggtgg cgagctcgcc cagcaccatg agcaggccgc catcgccggt gaagacgacg
     7081 accggccggt cgggctcggc gatcttggcg ccgatggcga gcggcaggcc gcagcccatg
     7141 gtgcagagcc cgttcgaatg gaggagggtg cggggcgcgt aggccggcca gacctggctc
     7201 aggaggatgc ggtgggcgcc gacatcggcg gccgccaccg tgtcgcgcgg cagcacccgg
     7261 cgggcgacct cgcagatcgc ggcggggccc catgcctcgt cgctgcggaa gagggcgagc
     7321 acctggtccc gcacggcgcc aaccgccgcc ggcgtccagg tcgaggcgga acgcacctcc
     7381 tggcccaggg cgtcgagccc ggcaccgata tcgcagacga agccgatccc cgcctggtgc
     7441 agatagtgat cgttggcgac ggcgtcgaag gcgatcaccc gcttctgggt ggtgtcccag
     7501 ggatgctgcc agtcgcggcg catctcgacc gcgtcgtagc cggcgagcac gacgaggtcg
     7561 gccttctcga tctcggcctc gagctggcgg tcggccaccg gcgagagggc cgcggcggcg
     7621 agggcgaaat cgtggtcctc gggcagcagg cccttggcct tgtaggtctg gatgacgggt
     7681 gcccgctggg tcagggcgaa acgggcgagc gctgctccgg catcgtgctg gacggcatcg
     7741 aagccggcga tgacgagtgg ccgcctcgcc tcggcgagcc aggtgcgcgc ctcgtcgagc
     7801 accgggccgg cggccggggc cacgggtgct gtcggggcgc ggcggatggg cgtggtcgcg
     7861 gctgccggcg cgtcggccac tgagatcggc acctcgatca gcaccgggcc gggccgcggg
     7921 tcgccggcca gcgccagggc ccgctcggtg acgaggtcgg cggcggtcgc ggtcagggtg
     7981 aaggcgccct tgatggcggg ctcgaggagc tttttgtgat cgatgatctg gtggttgtag
     8041 gcgagctgct cgtcggcatc gacggcgccc acgaggacga tcagcggcac ccggtcgagc
     8101 cgcgcgttgg cgacgacgtt gacggcgttg ctgatgccgg gcccgaccgt ggccaccagc
     8161 acgggcggcg tgccggtgcg ctgccagacc gcttcggcca tgaagcccgc cgccgtctcg
     8221 tgcttggtgg tgacgaaggt gatgccggcg gcggccagcg cgtcgatcag ggtgaggatc
     8281 tcgccgcccg gaatgccgaa ggcgaagcgg cagccggcct cggcgaggcg ccgggcgag
//
LOCUS       DAOTFI010000216         8334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10297_length_8334_cov_5.526030, whole genome shotgun sequence.
ACCESSION   DAOTFI010000216 DAOTFI010000000
VERSION     DAOTFI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10297_length_8334_cov_5.526030"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /gene="glgP"
                     /locus_tag="P5558_18705"
     CDS             complement(<1..1650)
                     /gene="glgP"
                     /locus_tag="P5558_18705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRY26406.1"
                     /translation="MRAIEPFLPRTRIAYFSMEMALQPEMHTYSGGLGVLAGDTARSC
                     ADLDLPVVFVTLASREGYLRQEIDAAGRQIDHADPWEPGDWATPLGAMVALAIEGRQV
                     WIRPWLHVLTCPVGHAVPVLLLDTRLEQNDPADRAITDRLYGGDEAYRLKQEIVLGIG
                     GERLLHALGFEIETYHLNEGHAALLTAALLRRHPHAGDRPAESALGFDADQVRERCVF
                     TTHTPVEAGHDRFGYALAERQLDLIPIDQLKLVAGADRLNMTRLALNLSGYVNGVALR
                     HAETTRQMFPGYRIRAVTNGVHVPSWAHPAFGRLFQGMAPDWGHDPEQLAIADQLPDA
                     DVSAAHTEAKSELIAEVKLRTGFQLQPDLPLLAFARRMTGYKRADLIFTDPERLRAIH
                     RRHPFQIVMAGKAHPRDEGGKVLIQRLHACLRELPDIPMAFLPGYDMALARSLVSGAD
                     VWLNTPLPPMEASGTSGMKAAVNGVLNLSVLDGWWIEACIEGVTGWAIGMDDRRDDDD
                     ALQLYEKLGNAVLPLYHEDRSRWVWMMKQAISKIAPRFNSQR"
     gene            complement(1650..2729)
                     /locus_tag="P5558_18710"
     CDS             complement(1650..2729)
                     /locus_tag="P5558_18710"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HRY26407.1"
                     /translation="MTADDASIRVAVNGYGVIGKRVADAVALQDDMALVGVADVSADW
                     RLRIPARKGFDLFAASPDREQAMREAGHEIAGSLDDLLGEADIVVDCTPKRVAAKNVE
                     LYRRQGIKFILHGGEKHEITGHSFVADANYASALDRDSTRVVSCNTTATVRTLGALKR
                     AGLLRRARGTLLRRATDPWESHLGGIMNTLVPEPEIPSHQGPDAQSVDPELDVITMAV
                     KVPETLAHLHYWSVQLTRQASKAEVLEAFATSTRIAMIRLDAGLVALNTVKELMADLG
                     RPHDNLYEVALWTDMLKVAGDELFYAYMVDNQAIVIPETIDAIRALTGKERDARASIA
                     KTDRALGIGAIAEALGASAPAPEDV"
     gene            complement(2829..3485)
                     /locus_tag="P5558_18715"
     CDS             complement(2829..3485)
                     /locus_tag="P5558_18715"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008282881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRY26408.1"
                     /translation="MPDNGSDYGLWSLVVLNSAIFVAFAFSFFKPSTTRDWRSFGAFS
                     AFLVALFTEMYGFPLTIYLLSGWLQSAYPGIDWLTHNAGHLPEMLLGWQANPHLGPFH
                     LLSFALIGGGFIVLSRAWPVLYAAQRQGRLATSGAYAHVRHPQYVGFVLILLGFLAQW
                     PTILTLAMFPVLVWMYARLARAEERAALAEFGDAYRAYADRVPAFIPRLGLSARRPVS
                     "
     gene            complement(3478..3744)
                     /locus_tag="P5558_18720"
     CDS             complement(3478..3744)
                     /locus_tag="P5558_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HRY26409.1"
                     /translation="MSFLSSRSGLVLIAFLAIGGYFLWAEHEAHVALVVPYLPWLLLL
                     ACPLLHFFMHGGHGHGHGGGVPGRVASNRDASAGRAPPAGGPDA"
     gene            complement(4056..4334)
                     /locus_tag="P5558_18725"
     CDS             complement(4056..4334)
                     /locus_tag="P5558_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26410.1"
                     /translation="MTLTTLLPQHGTADEAGGPATATVLLHSDTDSGGHGHAAGRACH
                     ASVGCVQLALAGTDLLPPQPSNAERIFVAVQQAPLSLRPAPDLKPPIT"
     gene            complement(4533..6992)
                     /locus_tag="P5558_18730"
     CDS             complement(4533..6992)
                     /locus_tag="P5558_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY26411.1"
                     /translation="MAEQAATLTAQVTRDPVCGMVVDPAAGTRTHEHDGHRYHFCSER
                     CHTKFAASPDEYISATDPVCGMTVHRATAQHLARHGDERFYFCSERCQQKFEVDPESY
                     RDGRPAAEPMPEGTIYTCPMHPEIEQVGPGDCPICGMALEPKGVPTGDEGPNPELVDF
                     RRRFWIGAVLTVPLLVLTMGPLVGLPVRMWLGERLTLWIELLLGTPVILWSGWPFLVR
                     GAKSFRTMNLNMFSLIGMGVAAAWAFSIVAVLVPGIFPDGFRDAEGHVGVYFEAGAVI
                     VVLVLLGQMLELGARERTGAAIRALLDLAAKTARVIRADGKEEEIPLEDVQLGDRLRV
                     RPGEKVPVDGEVVDGRSSVDESMLTGEPLPVEKVAGDPVTGATINGNGSLIIEAKRVG
                     KDTMLAQIVEMVAAAQRSRAPIQKLADAVAGKFVPAVIGIAAIAFIAWAIWGPAPSLS
                     YALVSAIAVLIIACPCALGLATPMSIMTATGRGAQAGVLVKNAEALERFAKVDTLIVD
                     KTGTLTQGKPKLVAVLPEAGHDEAEVLRLAASLERGSEHPLAEAIVQGAEERGVELVG
                     AGDFEAITGKGVKGTVDGKSVALGNARLMADMGLDSGELGRSADARRDEGETVMFVVL
                     DGAIAGLVSVADPVKETTPAALEALHKLGFRIIMATGDNERTAKAVAGRLGIDEIRAE
                     VLPEDKARIIKDLQAEGHKVAMAGDGVNDAPALAQADVGIAMGTGADVAIESAGFTLV
                     RGNLDGIVRARRLSLATMRNIKQNLFFALVYNAAGVPVAAGILYPFLGILISPIFAAA
                     AMSLSSVSVIGNALRLRRVGI"
     gene            7217..7510
                     /locus_tag="P5558_18735"
     CDS             7217..7510
                     /locus_tag="P5558_18735"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRY26412.1"
                     /translation="MAGLATVGGIVRAEPGAGYVGHHGMMEWGGWLMGPIMMVIFFSL
                     LVGAAFVIIRLLGLGTGNRGGAGSDRPLDILRERFARGEIDKAEFEERRKALG"
     gene            7541..7948
                     /gene="cueR"
                     /locus_tag="P5558_18740"
     CDS             7541..7948
                     /gene="cueR"
                     /locus_tag="P5558_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HRY26413.1"
                     /translation="MNIGDVAGRSGLPNKTIRYYEEIGLVRPLRKANGYREFQEHHLH
                     SLAFLARARSLGFTIDDCRTLLALYEDRHRSSADVRNIANEHLERINRKIEELMSMRA
                     TLELLVEQCHGDDRPDCPILDELASTTGLRDSA"
     gene            7983..8327
                     /locus_tag="P5558_18745"
     CDS             7983..8327
                     /locus_tag="P5558_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26414.1"
                     /translation="MTCVLRFVLAALPMLALWAMLAIAPSAHAHDVAQPTHGQALQDG
                     AGEPIDRHDHGGARHDRSCVSMTGHCGTSAVHDVAGASAVHTEALSATLSPDDRAFAG
                     RSPEADTPPPRV"
BASE COUNT         1348 a         2756 c         2898 g         1332 t
ORIGIN      
        1 gcgctggctg ttgaagcgcg gggcgatctt gctgatcgcc tgcttcatca tccagaccca
       61 gcggctgcga tcctcgtggt agagcggcag gaccgcgttc ccgagcttct cgtagagctg
      121 cagcgcgtcg tcatcgtcgc gccggtcgtc catgccgatg gcccagccgg tcaccccctc
      181 gatgcacgcc tcgatccacc agccgtcgag cacgctgagg ttgaggacgc cgttgaccgc
      241 cgccttcatg ccgctggtgc cggaggcctc catcggcggc aggggcgtgt tgagccagac
      301 gtcggcgccc gagaccaggg accgggcgag tgccatgtcg tagcccggca ggaaagccat
      361 cgggatgtcg gggagctcgc gcaggcacgc gtggaggcgc tggatcagga ccttgccgcc
      421 ctcgtctcgt ggatgcgcct tgccggccat cacgatctgg aagggatgcc ggcgatggat
      481 cgcccgcagc cgctcgggat cggtgaagat caggtcggcc cgcttgtagc cggtcatccg
      541 ccgggcgaaa gcgagcagcg gcaggtccgg ctgcaactgg aagccggtgc gcagtttcac
      601 ctcggcgatc agctcggatt ttgcctcggt gtgcgccgcc gagacgtcgg cgtcgggcaa
      661 ctggtcggcg atcgccagct gctccggatc gtgtccccag tcgggtgcca tgccctggaa
      721 cagtcgcccg aaggccggat gggcccagct cggcacgtgt acgccattgg tcaccgccct
      781 gatccggtag cctgggaaca tctggcgagt tgtctcggcg tggcggagcg ccacgccgtt
      841 gacgtagccg ctgaggttca gcgccaggcg cgtcatgttg agtcggtccg cgcccgccac
      901 cagcttcagc tggtcgatcg ggatgaggtc gagctgccgc tcggccagcg cgtaaccgaa
      961 acggtcatgg ccggcctcga ccggcgtgtg ggtggtgaag acgcagcgct cgcgcacctg
     1021 gtccgcgtcg aagccgagcg cgctctcggc cggccggtcg cccgcatgtg ggtgccgcct
     1081 gagcagggcc gcggtgagca gggccgcgtg gccctcgttg agatggtagg tctcgatctc
     1141 gaagccgagg gcgtgcagca gccgctcgcc gccgatcccg aggacgatct cctgcttcag
     1201 ccggtaggcc tcgtcgccgc catagagccg gtcggtgatc gcgcggtcgg ccggatcgtt
     1261 ctgctcgagc cgcgtgtcca ggagcaggac cggcacggca tggccgaccg ggcacgtcag
     1321 gacatgaagc cacggccgga tccagacctg gcggccctcg atcgccagcg cgaccatcgc
     1381 cccgagcggc gtcgcccagt ctccgggctc ccacgggtcg gcatggtcga tctgccggcc
     1441 cgccgcgtcg atctcctggc gcaggtagcc ctcgcggctg gcgagcgtga cgaagacgac
     1501 cggcaggtcg agatccgcgc aggagcgggc cgtatcgccg gcgagcacgc cgagcccgcc
     1561 ggaataggtg tgcatctccg gctgcagcgc catctccatc gagaaatagg cgatgcgggt
     1621 gcgcggcagg aacggctcga tcgcccgcat cacacgtcct cgggagccgg cgcgctcgcg
     1681 ccgagtgcct cagcgatggc gccgatcccg agggctctgt cggtcttggc gatcgacgct
     1741 cgcgcgtcgc gctccttgcc ggtgagtgcc cggatcgcgt cgatcgtctc gggaatcacg
     1801 atggcctgat tgtcgaccat gtaggcgtag aagagctcgt cgcccgccac cttcagcatg
     1861 tcggtccaga gcgccacctc gtagagattg tcgtgcggcc ggccgagatc ggccatcagc
     1921 tccttgaccg tgttgagcgc caccagcccg gcgtccaggc ggatcatcgc gatgcgcgtc
     1981 gatgttgcga acgcctccag cacctccgct ttgctggcct ggcgggtcag ttgcaccgac
     2041 cagtagtgca gatgggccaa ggtctcgggc accttcaccg ccatggtgat cacgtcgagc
     2101 tcgggatcga cgctctgggc gtcggggccc tggtggctcg ggatctccgg ctcgggcacc
     2161 agcgtgttca tgatgccgcc gaggtggctt tcccatgggt cggtggctcg gcggagcagc
     2221 gtcccgcgcg ctcgccgcag gagcccggcc cgcttgaggg cgccgagcgt gcggacggtt
     2281 gcggtcgtgt tgcaggagac gacgcgcgtg ctgtctcggt cgagcgcgct ggcgtaattg
     2341 gcgtcggcga cgaacgaatg gcccgtgatc tcgtgcttct caccgccgtg caggatgaac
     2401 ttgatgccct gccggcggta gagctcgaca ttcttggcgg ccacgcgctt cggcgtgcag
     2461 tcgacgacga tgtcggcttc gccgagcaga tcgtcgagcg agccggcgat ttcgtggccg
     2521 gcctcgcgca tcgcctgctc tcgatcaggg ctcgcggcga agagatcaaa acccttgcgc
     2581 gctggaatcc gcagccgcca gtcggcggag acgtcggcga cgccgaccag cgccatgtcg
     2641 tcctgcaggg ccacggcatc ggcgacgcgc ttgccgatca caccgtagcc gttcaccgcg
     2701 acgcgaatgc ttgcatcatc ggccgtcatg gttcgttgct ccttgactgt tggtgggtga
     2761 cttggggcgc tggtcccgca ccgcaggcgc gtatcggtcg cggaccagcg agctccgcat
     2821 tgccgctttc atgagaccgg acgcctggcc gacaggccca gccgggggat gaacgccgga
     2881 acgcggtcgg catatgcccg gtaggcgtca ccgaactcgg ccagggccgc gcgctcctcg
     2941 gcacgggcga gacgcgcgta catccagacg agcaccggaa acatggcgag cgtcaggatt
     3001 gtcggccact gggcgaggaa gcccagcagg atcaggacaa agccgacata ctgtggatgg
     3061 cggacatgcg cgtaggcgcc gctggtggcg agccggccct gtcgctgggc ggcgtagagc
     3121 accggccacg cccgggacag cacaatgaag ccgccgccga tcaaggcgaa gctcaagaga
     3181 tggaacggcc cgagatgcgg attggcctgc caaccgagga gcatctcggg caaatgtccg
     3241 gcattgtgcg tgagccagtc gatgcccgga taggcgctct gtagccagcc cgagaggaga
     3301 tagatcgtca ggggaaagcc gtacatctcg gtgaagaggg cgacgagaaa ggcgctgaac
     3361 gcgccgaagc ttcgccaatc gcgggtcgtg ctcggcttga agaagctgaa cgcgaaggcg
     3421 acgaagattg ccgagttgag gacgaccagc gaccacaggc cgtagtcaga gccgttgtca
     3481 ggcatctgga cccccagccg gcggcgcacg gccagcgctc gcgtcccggt tcgacgccac
     3541 tctgcccgga acgccgccgc catggccatg accatggccg ccgtgcatga agaagtggag
     3601 caaggggcag gcgagcagca ggagccaggg cagataagga accaccagcg ccacgtgcgc
     3661 ctcgtgctcc gcccagagga agtagcctcc gatcgccagg aaggcgatca gcacgaggcc
     3721 actgcgggac gagaggaagc tcatctcacc atcctcccga ctactaagtt ggcggggaac
     3781 gagagcacca gagggccgag aagctcggct actgccggta gtggttgggc cgatatggag
     3841 tgggaaggga gcattgatcc tgtcccgttc tgtggtcgtg ttgtgggagt gtcgccctgc
     3901 cggcaacgtc gtcgctgcgg ttcgcgagcc ttgcccggcc ggccgaaccg aaggtcgcgt
     3961 gtggccgtgg gaaggcattg gcgacgcacg acgaccggcc cggcacatgt gcgccgaact
     4021 cgagccgatg cgccgcggtc ggcccgcggc agcgctcagg tgatgggcgg cttgagatcc
     4081 ggcgccggcc gcaggctcag cggcgcctgc tggacggcga cgaagatccg ctccgcattc
     4141 gacggctgcg gcggaagcag atccgtcccc gccagcgcca gctgaacgca gccgaccgac
     4201 gcatggcatg cgcgacccgc ggcgtgaccg tgtccgcccg agtcggtgtc ggaatgcaag
     4261 agcactgtcg ccgttgccgg gccgcccgcc tcgtcagcgg tgccgtgctg cggcagcagc
     4321 gtggtcaggg tcatgagaag cgctgccagg acgcaagcga caagccaccg ctcccagcgc
     4381 ctgccaggct ggtcagtcgt cgccttgatc gtctttactt cgggcggcat cgcttcgagt
     4441 ctccggtcat ccagtctggc agttgcgcga actgccgcct tgatcgagat caatctcgtg
     4501 gccggtttcc tcgcggcgct ctgctccggc cctcatattc cgacccttct gaggcggagc
     4561 gcgttgccga tgacggagac cgaggagagg ctcatggctg cggcggcgaa gatcggcgag
     4621 atcaggatac cgaggaaggg gtagaggatg ccggcggcga ccggcacgcc ggcggcattg
     4681 tagacgagag cgaagaacag gttctgcttg atgttgcgca tcgtcgccag cgagaggcgg
     4741 cgggcgcgga cgatgccgtc gagattgccc ctgaccaggg tgaagccggc gctctcgatc
     4801 gccacgtcgg cgccagtgcc catggcgatg ccgacatcgg cctgggccag ggccggcgcg
     4861 tcgttgacgc cgtcgccggc catcgcgacc ttgtggccct cggcctgcag atccttgatg
     4921 atccgcgcct tgtcctcggg cagcacctcg gcgcggatct cgtcgatgcc gagccggccg
     4981 gccacggcct tcgccgtgcg ctcgttgtcg ccggtcgcca tgatgatgcg gaagcccagc
     5041 ttgtgcagcg cctcgagcgc cgccggagtc gtctccttga ccgggtcggc gacgctgacg
     5101 aggccggcga tggcgccgtc gagcacgacg aacatcaccg tctcgccttc gtcgcggcgg
     5161 gcgtcggcgg acctgccgag ctcaccgctg tcgagcccca tgtccgccat cagcctggcg
     5221 ttgccgagcg ccaccgactt gccgtcgacg gtgcccttga cgcccttgcc ggttatcgcc
     5281 tcgaaatccc cggcaccaac cagctcgacg ccgcgctcct cggcaccctg gacgatcgcc
     5341 tcggccagcg ggtgctcgga gccgcgctcg agcgaggcgg cgagacgcag cacctcggcc
     5401 tcgtcgtgcc ccgcctcggg caggacggcc acgagcttcg gcttgccctg agtgagcgtg
     5461 ccggtcttgt cgacgatcag cgtgtccacc ttggcgaagc gctcgagcgc ctcggcgttc
     5521 ttgaccagca cgccggcctg ggcaccccgg ccggtggcgg tcatgatcga catcggcgtg
     5581 gccaggccga gggcgcaggg acaggcgatg atgaggaccg cgatcgccga gacgagcgca
     5641 taggaaaggg agggtgccgg gccccagatg gcccaggcaa tgaaggcgat ggcagcgatg
     5701 ccgatgacgg ccggcacgaa cttgccggcg actgcgtcgg caagcttctg gatcggtgct
     5761 cgggaacgct gggcggcggc caccatctcg acgatctgcg ccagcatggt gtccttgccg
     5821 acccgctttg cctcgatgat caggctgccg ttgccgttga tcgtcgcccc ggtgaccggg
     5881 tcgcccgcga ccttttcgac cggcaagggc tcgcccgtaa gcatcgattc gtcgaccgag
     5941 gagcgcccgt cgacgacctc gccgtcgacc ggcaccttct cgcccgggcg aacccgcagc
     6001 cggtcgccaa gctggacgtc ctcgagggga atctcctcct ccttgccgtc ggcgcgaatg
     6061 acccttgccg tcttggccgc gaggtcgagc agggcgcgga tcgcggcgcc cgtgcgttcg
     6121 cgagcgccga gctcgagcat ctggccgagc agcaccagca cgacgatcac ggcaccggcc
     6181 tcgaaataga ccccgacatg cccctcggcg tcacggaagc catcggggaa gatgccgggc
     6241 accagcacag cgacaatgct gaacgcccag gcggccgcca cgcccatgcc gatcaggctg
     6301 aacatgttga ggttcatggt gcggaacgac tttgccccgc gcacgaggaa cggccagccc
     6361 gaccagagga tgaccggtgt cccgagcaga agctcgatcc agagcgtgag ccgctcgccg
     6421 agccacatcc ggaccggcag cccgaccagc gggcccatcg tgagcaccag gagcggcacc
     6481 gtcagcacgg cgccgatcca gaagcgacgc ctgaaatcga cgagctcggg attcggtccc
     6541 tcgtcgccgg tcggcacacc cttgggctcc agcgccatgc cgcagatcgg acagtcgccc
     6601 ggcccgacct gctcgatctc cgggtgcatc gggcaggtgt agatggtgcc ctccggcatc
     6661 ggctcggcgg cgggccggcc atcgcgatag ctctccgggt cgacctcgaa cttctgctgg
     6721 cagcgctcgg agcagaaata gaagcgctca tccccatgcc tggccagatg ctgcgccgtg
     6781 gcgcgatgga cggtcatgcc gcagacggga tcggtcgccg atatatactc gtcgggtgag
     6841 gccgcgaact tcgtgtggca gcgttccgag cagaaatgat agcgatgacc gtcatgctcg
     6901 tgcgtccgcg tgccggcggc cggatcgacc accatgccgc agaccgggtc acgggtgacc
     6961 tgggcagtga gggtggcagc ttgctcggcc atgtgaacct ccatcggcga accagagacc
     7021 ggtaagatgg gcattccagt cactggaagg tcaagggcta taaaattgct cgtgcgggat
     7081 cttgaccttc cagtggctgg aacgcccatc tgtagcccag tcgcatgtcg ggcgcagggc
     7141 aacttgccgc ttcgcctcgg gatgcggcgg cagagaaagg gaaagtaccg tgcacagagg
     7201 gcttgcagtg ttgaccgtgg ccggcctggc gacggtcggc ggtatcgttc gggccgagcc
     7261 gggcgccggt tatgtcgggc atcacggcat gatggagtgg ggcggctggc tgatggggcc
     7321 tatcatgatg gtgatctttt tcagcctcct cgtcggcgcg gccttcgtca tcatcaggct
     7381 gctcggcctc ggtactggta acagaggcgg tgccggctcg gaccggccgc tcgatatcct
     7441 gcgggaacgg ttcgcccgtg gggagatcga caaggcggag ttcgaggagc gtcgcaaggc
     7501 gctcggctga atggcaacgc cacgatggag gaatggacgc atgaatatcg gcgacgtggc
     7561 agggcggagc ggcctgccga acaagacgat ccgctactac gaggagatcg ggctcgtccg
     7621 cccgctgcgt aaagccaacg gctaccgcga gttccaggag caccacctgc acagtctggc
     7681 gttcctcgcc cgcgctcgct cgctcggctt caccatcgac gactgccgca cactgctcgc
     7741 gctctatgag gatcggcacc gatcgagcgc ggacgttcgg aacattgcca acgagcacct
     7801 cgagcgaatc aatcgcaaga tcgaggagct catgagcatg cgcgcgacgc tcgagttgct
     7861 ggtcgagcag tgtcacggcg acgaccggcc cgactgcccc attctggacg agctcgccag
     7921 caccaccggg ttgcgagatt cggcttgacg ttccagtagg tggaaagtgt aaacgacgtt
     7981 tcatgacctg tgtgctgcgt ttcgtgcttg ccgccttgcc gatgctcgcc ctctgggcga
     8041 tgctcgcgat cgcgccgtcg gcccatgccc acgatgtcgc gcagccgacg cacggccagg
     8101 ccttgcaaga tggcgcgggc gagccgatcg accggcacga tcatggcggt gcgcgacacg
     8161 accgttcctg cgtctcgatg accggtcatt gtggcacctc ggcggttcac gacgtcgccg
     8221 gcgcatctgc ggtacacaca gaggcgctat cggccacgct ttcgccggac gatcgggcct
     8281 tcgcgggacg ctcgcccgaa gcggacacac cccctcctcg agtctgacgg actt
//
LOCUS       DAOTFI010000217         8282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10380_length_8282_cov_6.422633, whole genome shotgun sequence.
ACCESSION   DAOTFI010000217 DAOTFI010000000
VERSION     DAOTFI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10380_length_8282_cov_6.422633"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="P5558_18750"
     CDS             <1..211
                     /locus_tag="P5558_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26415.1"
                     /translation="DLVPERPGVTEEVLCVAAGSEIGRQLPPQLQAEDLTPLPVGSID
                     EVEALFRQSSPAGIASAKLPITITN"
     gene            complement(341..1126)
                     /locus_tag="P5558_18755"
     CDS             complement(341..1126)
                     /locus_tag="P5558_18755"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRY26416.1"
                     /translation="MKRMGFAILLLSWLVSGSAGAAATTVEHGPFTVDRVALLGLRGT
                     LRVEVVAGATTTLHVEGPEAAVAALEVTEGGGGLEVRAPAGGNSVTVIRNLTVVTGPG
                     ASSSVTIGGAAGSAAETEPLDLVLELPKGTGLTLDGFVGEAEIGDLEAEVAIGVVEGR
                     VTVGAVRSAGLATVGAATIGAASVDGDLEVSVTGDGAVEVAAGEVGAARLTLTGAGSI
                     TVDAPIETAEVSMVGAGTVRLAKVAAEPSVQRVGAGELHVGPP"
     gene            1188..1490
                     /locus_tag="P5558_18760"
     CDS             1188..1490
                     /locus_tag="P5558_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26417.1"
                     /translation="MAALTFALVMSASGAPALAEVTVGGERAGSGVARPGALEGRVSA
                     RCWQDGLQVLAIDDFAAADVGLELKELSLGLQGGGGKRSAVIVPLADSLCLLTISP"
     gene            1534..2211
                     /locus_tag="P5558_18765"
     CDS             1534..2211
                     /locus_tag="P5558_18765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY26418.1"
                     /translation="MRTTLHRLPLMALLLTTALAWTPMAAIAQDTVIFQDVPTVDELN
                     AVLFGKPADPAAPPIKTRAIRFHGAAAEAPPAPAAVQAQVPAPSAPAPAPEAAVASTE
                     PIAPAPPPPAGTALGMNILFEYNSTNVLPDSLPYLERLGEVLNLPENQGKVINIIGHT
                     DATGSSAYNQGLSEQRALAVGQYLVDVWQISPERLGVEGRGENQPLAGTDPNDGVNRR
                     VEFYALE"
     gene            2224..2940
                     /locus_tag="P5558_18770"
     CDS             2224..2940
                     /locus_tag="P5558_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26419.1"
                     /translation="MTELPAPATTEPPLPDKEAPDKGVNPAAGNRRAALLLSLWQVRA
                     DLYRSLEEHTRRALAGSNTIGPDELEDFIEQQVTTEQTEYIAQFLFVLRTAGCTEPGP
                     LGLYIDSHNAMIDRLLAELENARDTGRPVGSRLKQRLWRLRSARFNERMKAGTLERLG
                     EGRLVLSLKDLERFMAMHMDPTLCRDRLDALVKAGLLADEVRPNIRLIWSDGALEAIV
                     GRHLDDLWRQLKETALAPPL"
     gene            2924..3262
                     /locus_tag="P5558_18775"
     CDS             2924..3262
                     /locus_tag="P5558_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26420.1"
                     /translation="MPPRSSLVVAAACLALLTGPASATDLRIQLNPALAALAEQDPAA
                     AQQALDDLLTVIETGKAANGAPPKTRGMLDPTEQRLIEVNPLIQEAYRIDPKAALEQL
                     RQIVAAGAGG"
     gene            complement(3566..3799)
                     /locus_tag="P5558_18780"
     CDS             complement(3566..3799)
                     /locus_tag="P5558_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRY26421.1"
                     /translation="MSIRNGAFSGFGTAFGAGFELRAKRLVALLDVWARRRRDRRVLA
                     ELDDRLLRDIGIDRQAAMREAERPFWDGGAPRR"
     gene            3881..5272
                     /locus_tag="P5558_18785"
     CDS             3881..5272
                     /locus_tag="P5558_18785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY26422.1"
                     /translation="MTITIDDVSARPGPRYRALADSLETAIRTARYAPGARLPPQRDL
                     AHALDVTVGTVGRAYGLLLQRGLVRGEVGRGTFVQEPGRREAELLGHVPPEPGRRHDL
                     SVNRPVAVPAQARVFDVLASIGADGQRLAHLLTYPPHNGHLEHRLGLARWLAGFAGPL
                     DPERILPTLGTQNGMAVALAAATRPGDVLLMEAVAYPSNHGLAGRLGLKVEPVAMDAE
                     GALPESIGALCRQHRPTAIVLSPDLHNPTFATASAARREAIVAVARRHDLQIVEDAVY
                     APVAGTGLATLQSLAPERTHLVTSISKFLAPGLRMGCVVAPPDRLHRVVAAQAQLSLG
                     VSPIIAETFARLEAEGIVAEAILQQREALAHRRAVALAALRGLAVTSHANALHVLLDL
                     PPGQDAVGTAQRLAAIGLRTTPFAAFVIGRGVGRAALRLSLGGAADEATLAAALVALR
                     EHVSAGLDAPPVI"
     gene            complement(5288..6265)
                     /locus_tag="P5558_18790"
     CDS             complement(5288..6265)
                     /locus_tag="P5558_18790"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRY26423.1"
                     /translation="MGDPRGYYRLLGVPRSASAEEIRLAFRARAMASHPDRQGASGGD
                     DEVFRLVREAYEVLRDPLKRMAYDADGLDAEHAQAAARAAGGNGQERRERVAREQRPA
                     AARGTARKAAIERWLGRAVPLATGALALALVVAMASLWSARRQLEEREILLADLYGRL
                     NRTAAVEAELRAQLRSATFLDLERAMRRGPRLTGDVGRYAYAETIDFPAGRVGLGAAQ
                     EDRLTLAIVELAQRIEIIPGDVDWLIVLETQAAAAADGDGVAVEAWEPALLRLASVLD
                     HLLAQGLPSERMAIRFNAGFAAYPAPGAEPDGPAEMRDAVAVRLVCCVD"
     gene            6524..6877
                     /locus_tag="P5558_18795"
     CDS             6524..6877
                     /locus_tag="P5558_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26424.1"
                     /translation="MLQPTAGPFLSLAACAALIDEVYRWRERPAPGDSAWQPPVLGDG
                     RGRRHACGSREIIKLPRWARTRAIVLHECAHGMSADGHGPGFVRAYVGLLCTFAGHER
                     AVLEASLREHGVRIA"
     gene            complement(6874..>8282)
                     /locus_tag="P5558_18800"
     CDS             complement(6874..>8282)
                     /locus_tag="P5558_18800"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HRY26425.1"
                     /translation="IGGSSSINGMVYVRGHAMDYDGWEAMGAVGWGYRHVLPYFMRLE
                     ASHGGETGWRGTEGPLHVTRGRLANPLYGAFVKAGSEAGYAVTDDYNGRQQEGFGPLE
                     MTVWRGRRWSAANAYLRPALLRANLDLEARSLVRRVLLEGRRAVGVEVERQGQVRRIR
                     ARRAVALAAGAINSPRLLMQSGIGDAAHLAELGITPVIDRKGVGANLQDHLELYIQLA
                     CKQPITLFRYWNLPAKAAIGAWWLLTGKGPGASNHFEAGGFIRSAAGVAYPDIQFHFL
                     PLAVRYDGKASAAGHGFQAHVGPMRSKSRGRVRLTANEPGAPPSIRFNYLSDSQDLAD
                     FRTCIRLTRELFGQSAMARYAGEELQPGAAVQDDAALDAFIRSHAESAYHPCGTCRMG
                     IADDPMAVVDPECRVIGIDNLRVADSSIFPQITNGNLNAPSIMVGEKAADHIRGRTPL
                     PASELRPWIHPDWRNAQR"
BASE COUNT         1193 a         3168 c         2791 g         1130 t
ORIGIN      
        1 cgatctcgtg ccggaacgac ccggcgtcac cgaggaggtg ctctgcgtcg ccgccggcag
       61 cgagatcggc cgccagcttc cgccacagct ccaggccgag gacctgacac ccctgccggt
      121 cggcagcatc gacgaggtgg aggccctgtt ccgccagtcg agcccggccg gcatcgcctc
      181 ggcgaagctg ccgatcacga tcacgaactg acgatcacca gcaactgcgc ggtccgggcg
      241 atggcccgga ccgccagacc gggacgacca tgatgtccag acccgacccg ctcgccagga
      301 gcgtgaaccc tgtccaaccg accggccccc gaaacgccgc tcagggcggc ccgacatgca
      361 gctcgccggc gcccacgcgc tgcacgctcg gctcggccgc caccttggcg agccgcaccg
      421 tgccggcccc gaccatgctg acctcggccg tctcgatcgg ggcatcgacg gtgatcgagc
      481 cggcgccggt tagggtgagc cgggcggcac caacctcgcc ggccgccacc tcgaccgctc
      541 cgtcgcctgt gacgctgacc tcgagatcgc catcgacgct cgccgccccg atcgtggcgg
      601 cacccaccgt ggcgagcccg gcgctccgca ccgccccgac tgtcacccgg ccttcgacca
      661 cgccgatcgc gacctccgcc tcgagatcgc cgatctcggc ctcgccgacg aagccgtcga
      721 gcgtcaggcc ggtccccttc ggcagctcga gcacgagatc gagcggctcc gtttccgccg
      781 ccgagccggc agcaccgccg atcgtcaccg acgagctggc acccggcccg gtgacgacgg
      841 tcaggtttct gatcacggtc accgagttgc cgccagccgg ggcgcggacc tcgagcccgc
      901 cacccccctc cgtcacctcg agcgctgcca ccgccgcctc gggcccctcg acatgcaagg
      961 tcgtcgtcgc acccgcgacc acctcgaccc gcagtgtgcc gcgcaggccc agcagagcca
     1021 cgcggtcgac cgtgaagggc ccatgttcga ccgtggtcgc ggcagcaccg gccgatccgg
     1081 acacgagcca gctcaacagc aagatggcga aacccatgcg tttcatcgct tcgctcccgg
     1141 ctatccgcag tctcgggcga tcctaccaga aagcgggtgc cttacccatg gcagccctga
     1201 ccttcgcact cgtcatgtcc gcgagcggcg cgccggccct ggccgaggtc accgtcggtg
     1261 gcgagcgggc cggcagcggc gtggcccgac ctggcgcgct cgagggccgc gtttccgccc
     1321 gctgctggca ggacggcctg caggtcctcg ccatcgacga tttcgctgcg gccgacgtgg
     1381 gcctggagct gaaggagctg tcgctcggcc tccagggtgg cggcggcaag cgcagcgccg
     1441 tcatcgtccc gctcgccgat tcgctctgcc tgttgaccat cagcccctga gccgccaagc
     1501 cttgcggcca ccggacccga agggagatcc gtcatgcgaa cgaccctgca ccgcctgcct
     1561 ctcatggcgc ttctgctgac cacagcgctc gcctggacgc caatggccgc catcgcccag
     1621 gacacggtca tctttcagga tgtgccgacc gtcgacgagc tcaatgccgt gctcttcggc
     1681 aagccggccg atccggcggc gccgccgatc aagacccgcg ccatccgctt tcatggcgca
     1741 gccgcggagg ccccgccagc ccccgctgcc gtccaggccc aggtgccggc cccgtcggca
     1801 ccggcacccg ccccggaggc cgcggtcgcc agcacggagc ccatcgcgcc ggcaccgccg
     1861 ccgcccgccg gcacggcgct cggcatgaac atcctcttcg agtacaactc gaccaacgtg
     1921 ctgccggatt ccttgccgta tctggagcgg ctcggcgagg tgctgaacct gcccgagaac
     1981 cagggcaagg tgatcaacat catcggccac accgatgcca ccggcagcag cgcctacaac
     2041 caggggctct cggagcagcg cgcgctcgcc gttgggcagt acctggtcga cgtctggcag
     2101 atctcgcccg agcggctcgg ggtcgagggt cggggcgaga accagcccct ggccggcacc
     2161 gatcccaatg acggcgtcaa ccgccgggtc gagttctacg cgctcgaata aggtggcaag
     2221 acgatgaccg agctgcccgc acccgccacg accgagccgc ctttgcctga caaggaggcg
     2281 cccgacaagg gggtcaaccc ggccgccggc aaccggcgcg ccgccctcct gctctcgctc
     2341 tggcaggtgc gggccgacct ctatcgcagc ctcgaggagc atacgcgacg ggcgctggcc
     2401 ggcagcaaca ccatcggccc cgacgagctc gaggacttca tcgagcagca ggtcacgacc
     2461 gagcagacgg agtacatcgc ccagttcctc ttcgtcctgc gcaccgccgg ctgcaccgag
     2521 cccggaccgc tcggcctcta tatcgacagc cacaacgcca tgatcgaccg gctgctggcc
     2581 gagctcgaga atgcgcgcga caccggccgg ccggtcggct cgaggctcaa gcagcggctc
     2641 tggcgattgc gctcggcgcg tttcaacgaa cggatgaagg ccggcacgct cgagcgcctg
     2701 ggcgagggcc gcctcgtcct cagcctcaag gatctcgagc gcttcatggc gatgcacatg
     2761 gacccgacgc tctgccgcga ccggctcgac gcgctcgtca aggccggcct gctcgccgac
     2821 gaggtgcggc ccaatatccg cctgatctgg tccgatggtg cgctcgaggc catcgtcggc
     2881 cggcatctcg acgatctctg gcggcagctc aaggagacgg cccttgcccc cccgctctag
     2941 cctcgttgtc gctgccgctt gcctggcact gctcaccggg ccggcatcgg ccacggacct
     3001 gcgcatccag ctcaaccccg ccttggcggc cctcgccgag caggacccgg ccgccgccca
     3061 gcaggcgctc gacgatctcc tgacggtgat cgagacgggc aaggcggcga acggcgcccc
     3121 gcccaagacc aggggcatgc tcgatccgac agaacagcgc ctgatcgagg tcaacccgct
     3181 catccaggaa gcctaccgca tcgatcccaa ggccgccctc gagcagctcc gccagatcgt
     3241 cgcggccggc gccggcggct gactccgcca gccgccgcct ccggcgccgt cgtaaggcgg
     3301 cccttgccaa ccgggccggc cacacccgcg accgtgaccc gctcgagtcg cccggcccgc
     3361 ttcatcgccg tcacgtcgat ggtgccgggt cacgccgttt ccgcgcccag gacgatccgc
     3421 cagtggcggc cgagatcgcc ctccgccgcc accgcctgcg tcgggccggt tgtgaggcgc
     3481 acggcctcgc cgaccaccgc gagcgggttg ccggcgaagc gccgctcggc gaagacgtcg
     3541 aggattcaga acggcaggcc gccggtcacc ggcgcggcgc cccgccgtcc cagaacggcc
     3601 gctcggcctc gcgcatcgcc gcttggcggt cgatgccgat gtcgcgcagc aggcggtcgt
     3661 ccagctccgc cagcacccgc cggtcgcgcc gccggcgcgc ccagacatcg aggagcgcca
     3721 ccagccgctt cgccctgagc tcgaagccgg ctccgaaagc cgtgccgaac cccgagaagg
     3781 caccattgcg aatgctcatg atggctgctc catcgccgaa attgaaccgg ccatatgagt
     3841 catctggaca gacgaacgag tcaaacgata cattgttatc atgacaataa cgatcgacga
     3901 tgtctccgcc cgccccggcc cgcgctatcg cgccctggcc gacagcctcg aaaccgcgat
     3961 ccgcacggcg cgctatgcgc cgggcgcccg gttgccaccg cagcgcgatc tcgcccatgc
     4021 gctcgacgtg accgtcggca cggtcggccg cgcctatggc ctgctgctgc agcggggtct
     4081 ggtgcgcggc gaggtcggcc gcggcacctt cgtgcaggag cccggccggc gtgaggcgga
     4141 gctgctcggc catgtgccgc ccgagcccgg ccgccggcac gacctctcgg tcaaccgccc
     4201 cgtggccgtg cccgcccagg cccgggtctt cgacgtgctc gcgtcgatcg gcgccgacgg
     4261 ccagcgcctc gcccacctcc tcacctatcc gccgcacaac ggccatctcg agcaccgcct
     4321 gggcctcgcc cgctggctcg ccggcttcgc cgggccgctc gatccggagc ggatcctgcc
     4381 cacgctcggc acccagaacg gcatggcggt cgccctcgcc gcggccaccc ggccgggcga
     4441 cgtgctgctg atggaggcgg tggcctaccc gagcaatcac ggcctcgccg gccggctcgg
     4501 gctcaaggtg gagccggtcg cgatggatgc ggaaggggcg ctgccggaga gcatcggcgc
     4561 gctctgtcgt cagcatcggc cgaccgccat cgtgctgagc cccgacctcc acaacccgac
     4621 cttcgccacc gcctcggcag cgcggcgcga ggccatcgtc gccgtcgccc gccggcacga
     4681 cctgcagatc gtcgaggacg cggtctacgc cccggtcgcc ggcaccgggc ttgcgaccct
     4741 gcagagcctg gcacccgagc gcacccatct ggtcaccagc atctccaagt tcctggcgcc
     4801 gggtctgcgc atgggctgcg tggtggcccc tcccgatcgt ctgcacaggg tcgtcgccgc
     4861 ccaggcgcag ctctcgctcg gcgtctcgcc gatcattgcc gagaccttcg cccggctcga
     4921 ggccgagggc atcgtcgccg aggccatcct ccagcagcgc gaggccctgg cgcatcgtcg
     4981 ggcggtggcg ctcgcagcgc tgcgcggcct tgccgtcacc tcgcacgcca acgcgctgca
     5041 tgtcctgctc gacctgccgc ccggccagga cgcggtcggc accgcccagc ggctcgcggc
     5101 aatcggcctg cggaccacgc cgttcgcggc cttcgtcatc ggccgcggtg tgggccgggc
     5161 agcgcttcgc ctgtcgctcg gcggtgccgc ggacgaggcc actctcgccg ccgccctcgt
     5221 ggcgctccgc gagcacgtca gcgccgggct cgacgccccg ccggtgatct gaggcagtcg
     5281 gtccgcctca gtcgacgcag cagacgagcc tgacggccac cgcgtcgcgc atctccgcgg
     5341 gtccgtcggg ctcggccccg ggcgccggat aggcggcgaa gccggcattg aagcggatcg
     5401 ccatgcgttc gctcggcaga ccctgggcca ggagatggtc gagcaccgaa gccagcctca
     5461 gcagcgccgg ctcccaggcc tcgaccgcca ccccgtcgcc atcggcggcg gcggcggcct
     5521 gcgtctcgag cacgatgagc cagtcgacat cgcccgggat gatctcgatc cgctgggcga
     5581 gctcgacgat cgccagtgtc aggcggtcct cctgcgcggc gccgagcccg accctgccgg
     5641 ccggaaagtc gatggtctcg gcataggcat agcggccgac atcgccggtg agccggggcc
     5701 cgcgccgcat cgcccgctcg aggtcgagaa aggtggcgct gcgcaattgc gcccggagct
     5761 cggcctccac cgcggccgtc cgattgaggc ggccatagag atcggcgagc aggatctcgc
     5821 gctcctcgag ctggcggcga gccgaccaga gcgacgccat ggcgacgacc agcgccagcg
     5881 ccagggcgcc ggtggccagc ggcacggcac ggccgagcca gcgctcgatg gccgccttgc
     5941 gcgccgttcc ccgggcagcc gcgggccgct gctcccgggc gacccgctcg cgtcgctcct
     6001 ggccattccc acctgccgcc cgggccgccg cctgcgcatg ctcggcatcg aggccgtcgg
     6061 cgtcgtaggc catgcgcttg aggggatcgc gcagcacctc gtaggcttcg cgcaccaggc
     6121 ggaacacctc gtcgtcgcca ccgctcgcac cctgccggtc ggggtgcgag gccattgccc
     6181 gcgcgcgaaa ggcgagccgg atctcctccg ccgaagcgga gcggggaacg cccagaaggc
     6241 gatagtagcc tcggggatcg cccatggcgg acacgcgtcg cacctctgca atggctgcgc
     6301 ggaattcgct ggaaggcgcc gggtggcgcg tcggatgagc tggcggaatt gcccgaaacc
     6361 ggcgaaaagg caagcctgtg atcccgagcg aggccgaggt ggcgtgctgg accggttgtt
     6421 ttccctgtgg cgtgaggcgc cggcgcaacc gctgcccagg cctcgccggc cgaagcgccc
     6481 gcgtgatttc cagcgacgcc gggtctatgg ctgggaggcg gcggtgctgc agccgacggc
     6541 cggcccgttt ctctcgctcg cagcctgcgc ggcgctgatc gacgaggtct atcgctggcg
     6601 cgagcggccg gcgccgggcg acagcgcctg gcagccgccg gtgctcggcg atggccgtgg
     6661 ccggcggcat gcatgcggct cgcgcgagat catcaagctg ccccgctggg cccgcacgcg
     6721 tgccatcgtc ctgcacgaat gcgcccacgg catgagcgcc gacggccacg gcccgggatt
     6781 cgtccgcgcc tatgtcgggc tgctctgcac cttcgccggc cacgagcgag ccgtcctcga
     6841 ggcctcgctc cgcgagcatg gcgtgcggat cgcctagcgc tgggcgttgc gccagtcggg
     6901 atggatccag ggccggagct ccgatgccgg cagaggcgtg cggccccgga tatggtcggc
     6961 ggccttctcg cccaccatga tcgagggcgc gttgagattg ccgttggtga tctgcggaaa
     7021 gatcgagctg tcggcgaccc ggaggttgtc gatgccgatc acccggcact ccggatcgac
     7081 cacggccatg gggtcgtcgg cgatgcccat ccggcaggtg ccgcaggggt gataggcgct
     7141 ctcggcatgg cttcggatga aggcgtcgag cgccgcatcg tcctgcaccg ccgcccccgg
     7201 ctgcagctcc tcgcccgcgt agcgcgccat cgccgactgg ccgaagagct cgcgggtgag
     7261 ccggatgcag gtgcggaaat cggcgaggtc ctgcgagtca ctcagataat tgaatcgaat
     7321 cgatggcggt gcgccgggct cgttcgccgt cagccggacc cggccccggc tcttcgaccg
     7381 catcggcccg acatgcgcct ggaagccgtg tcccgccgcc gaggccttgc cgtcgtagcg
     7441 cacggcgagc ggcaggaaat ggaactggat gtcgggatac gcgacgccgg cggccgagcg
     7501 gatgaagccg ccggcctcga aatggttgct ggcccccggc cccttgccgg tgagcagcca
     7561 ccaggcgccg atcgccgcct tggccggcag gttccagtag cgaaagagcg tgatcggctg
     7621 cttgcaggcg agctggatgt agagctcgag atggtcctgc aggttggccc ccacaccctt
     7681 gcggtcgatg acgggcgtga tgccgagctc cgccagatgg gccgcgtcgc cgatgcccga
     7741 ctgcatcagc agcctgggcg aattgatcgc cccggcggcg agcgccacgg cccgccgggc
     7801 ccggatccgg cgcacctgcc cctgccgctc gacctcgaca ccgaccgcgc ggcggccttc
     7861 gagcaggacc cggcgcacga ggctcctggc ctcgaggtcg agattggcgc gcagcagggc
     7921 gggtctgaga taggcgttgg ccgccgacca gcgccgaccc cgccagacgg tcatctcgag
     7981 cgggccgaag ccttcctgct gccggccgtt gtagtcgtcg gtcacggcat agccggcctc
     8041 gctgccggcc ttgacgaagg cgccatagag cgggttggcg aggcgccccc gggtcacgtg
     8101 cagggggccc tcggtgccgc gccagccggt ctcgccgccg tggctcgcct ccagccgcat
     8161 gaagtagggc agcacgtggc gatagcccca gccgacagca cccatggcct cccagccgtc
     8221 atagtccatg gcgtggccgc gcacatagac catgccgttg atcgaggagg agccgccgat
     8281 ca
//
LOCUS       DAOTFI010000218         8261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10414_length_8261_cov_7.775165, whole genome shotgun sequence.
ACCESSION   DAOTFI010000218 DAOTFI010000000
VERSION     DAOTFI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10414_length_8261_cov_7.775165"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="P5558_18805"
     CDS             <1..602
                     /locus_tag="P5558_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY26426.1"
                     /translation="AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIE
                     TINKLVRASRQMLHEYGREPTPEELAHKLNMPLDKVRKVLKIAKEPISLETPIGDEED
                     SHLGDFIEDKNAVQPLEAATLANLRDATTRVLATLTPREERVLRMRFGIGMNTDHTLE
                     EVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDT"
     gene            759..1154
                     /locus_tag="P5558_18810"
     CDS             759..1154
                     /locus_tag="P5558_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26427.1"
                     /translation="MPADLAVEEAHPTEPVVLVNIAREWDPALTDRDLYERSRRYWRC
                     SPQRHPANYAMVVGDGIIREVYRIDGWHEVDMAAAELDPGRKGAAEPLPRHTRRQEFD
                     DEIALHMRHYVGKSARHLLGQNPFRWLNC"
     gene            complement(1214..1648)
                     /locus_tag="P5558_18815"
     CDS             complement(1214..1648)
                     /locus_tag="P5558_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26428.1"
                     /translation="MKRHLTIMAAAAFLAACGTTADDSPWRSKVTTPAAHRITGQEIA
                     VGDFTGTSGRTTSGEVAVFQTTAGYAVSLGSDFSIDNAAGATVGLGDAAGATVVLAPL
                     ASPTGAQVYGVPAGVDIGQFRHVYIWDDARDVPLGVAQLDLL"
     gene            complement(1804..2067)
                     /locus_tag="P5558_18820"
     CDS             complement(1804..2067)
                     /locus_tag="P5558_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY26429.1"
                     /translation="MPIVVTLDVMLARRKARSCDLAAFVGITEANLSLFKQGKVKGVR
                     FATLARICTFLDCQPGDLLMHVAEDAPDDGNGDPARPADRIPG"
     gene            complement(2080..2619)
                     /locus_tag="P5558_18825"
     CDS             complement(2080..2619)
                     /locus_tag="P5558_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26430.1"
                     /translation="MQRGGADARLRRLSGRLATAALVLAVVLPLLVLWFWLAGDPSAQ
                     FARVAGARLTHAPEPWQVGMAAFLALLPSLILSTALLAAWRCFRGFAAGSYLTRDAAA
                     ALRRMGIRVGLAAGLGLVMPTVLGLVLTANAGPGQHQLVISLGSDQALGVLIGGMVWA
                     IGEVLERAAMLADDHAQIV"
     gene            2855..2930
                     /locus_tag="P5558_18830"
     tRNA            2855..2930
                     /locus_tag="P5558_18830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2888..2890,aa:Ile,seq:cat)
     gene            3125..6091
                     /locus_tag="P5558_18835"
     CDS             3125..6091
                     /locus_tag="P5558_18835"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRY26431.1"
                     /translation="MRYLIGGLAALAVAIVAAVLLVQHFFDERLLALRLQQAVADATG
                     IELVIEGGLDLSLLPRPVLSVHRLRLDDAAGDLLLTADRLDLDLELRPLLAGELGVTD
                     AHLVRPDLRVIGNPATAASRLAAFLGASLPGEAVQRIRIADGALQVTGADGAAPLVWR
                     GIDAVLTGDRVTGTSHLAAGAAFAGVAAGPRLVLGGELGSAAPGRPRALRLDLALEGT
                     ASAVLGYRGQLRPDGGPDRQGALLDGRLTLDLPDPAGLRDWLALVAPALAAWPVPEAP
                     LGLEAELSFAPAAAADPMFLRLGGLALTVAGQTLDGALDLVGGEAPVLDLQLRAGRLE
                     LPDDWPAAGDPRRLAAVLPADLRGHLELEVDQLDWRRRSFRQIAIGLGLEGDGDVRVE
                     RAVAVLPGPGDLDFTGRVGPLGSATGHWLSGRLQAALQVPGELLALFGEPPAMLRQST
                     TLALDTDLDWSAAALTLRNADLSLDATRVEGGIAWRPAVDGKRAQLALRLMLDRLDLG
                     EVVDVHDPRATIDTLEAILPATDYAVDLKIERASLDERRLGALTLRLDSVDGEVAISR
                     ASLDGLAGSALMLSGRVTPASRAFDLDLAFDIASLGRLLRLVDYPAPPALALLGPVNL
                     RASLTGDAASTTIDATLDGDSFGASASGVLDDWTTARLGGTLALTLGAADAAGVLRQL
                     GGVPITAAVFTGPLDADLQLLLTDGRPTAWNLEASLGGLDLDLVREPGTEGEAERLDL
                     AIGPLSPAAAAVVYELATPLLQLVPGPPGRWLGFWPDQSLRFDWLDGEPRAIRIELVP
                     EASPAAPVVIEADLADGVLAVPAFAWENDGMRLAGSMALEHRHAGAAAALSLDLALDG
                     ATATRLGELLDLPAGVTGEVGLEARVTSLGRSVRTLIGNASGEAAIALENGAIGAVPV
                     DRLAGDLMLERGVLRPVGDGIPFAGPEGSGSVTGAVDLLAWLADLELELTPAAPGAPA
                     RHQRLLDGLRTPAPSD"
     gene            complement(6096..7493)
                     /gene="fumC"
                     /locus_tag="P5558_18840"
     CDS             complement(6096..7493)
                     /gene="fumC"
                     /locus_tag="P5558_18840"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRY26432.1"
                     /translation="MTSNVRIETDTFGPIEVEASRYWGAQTQRSLQNFRIGGERMPLP
                     LVRALGVVKRAAAEVNQAQGSLDGRLAEAIGAAAQEVIDGKLDDHFPLVVWQTGSGTQ
                     SNMNANEVIANRATELLGGERGQKSLVHPNDHVNRGQSSNDTFPTAMHIAATQEVHER
                     LLPALGHLKDALAAKAAAFEKIVKIGRTHLQDATPLTLGQEFGGYVQQLAYGIERVRG
                     TLPRISQLAQGGTAVGTGLNSKKGFDVAFAAAASRITGLAFETAPNKFEALAAHDALV
                     ELSGALNTVAVSLMKIANDIRLLGSGPRCGLGELKLPENEPGSSIMPGKVNPTQCEAM
                     TQVCAQVMGNHVAVTVGGATGHFELNVFKPVIIYNVLQSIRLIADASVSFADNCVVGI
                     TADEARIEKLLHESLMLVTALNPRIGYDNAAKVAKKAHAEGTTLKESALALGLLDEAE
                     FDALVRPERMLGPSD"
     gene            complement(7594..8106)
                     /locus_tag="P5558_18845"
     CDS             complement(7594..8106)
                     /locus_tag="P5558_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HRY26433.1"
                     /translation="MRRQTIDYGQLVETALRTVVRDVLRRFAAGDVPSPHHFYVTFRT
                     DMPGVEIPDFLRSRYPNEMTIVLQHQFWDLEVEDDKFAVTLSFNDQPERLVVPFEALR
                     VFADPGVEFGLQFTLGAEKDGEEPVSLPLTAKTGAKPRPVASEDEAPAEPPSNEGGAE
                     IVTLDRFRKK"
BASE COUNT         1173 a         3045 c         2808 g         1235 t
ORIGIN      
        1 aggcggtcga caagttcgag taccgccgcg gctacaagtt ctcgacctac gccacctggt
       61 ggatccgcca ggcgatcacc cgctcgatcg ccgaccaggc ccggaccatc cgcatcccgg
      121 tccacatgat cgagacgatc aacaagctgg tccgggcgtc ccgccagatg ctgcacgagt
      181 acggccgcga gccgacgccc gaggagctcg cccacaagct caacatgccg ctcgacaagg
      241 tcaggaaggt cctgaagatc gccaaggagc cgatcagcct cgagacgccc atcggcgacg
      301 aggaggacag ccacctcggc gacttcatcg aggacaagaa cgccgtccag ccgctggaag
      361 ccgccaccct cgccaacctc cgcgacgcca ccacccgcgt cctcgccacc ctcacccccc
      421 gcgaggagcg cgtgctgcgc atgcgcttcg gcatcggcat gaacaccgac cacaccctcg
      481 aggaggtcgg ccagcagttc agcgtcaccc gcgagcgcat ccgccagatc gaggccaagg
      541 cgctccgcaa gctcaagcac ccgagccggt ccaggaagct caggagcttt ttggatacct
      601 gagatgcgaa caaggtcggg gagggtgacc ctccccgtca gccccaccgg tcttttggtt
      661 tcggtttttt gacggcccgg ctgccgggcg cttgtagtga ccaataatcc aatctaaaat
      721 tgaattatgg agatttaatt cgacctggga agtgggccgt gccggcagat ctggctgtcg
      781 aagaggcgca cccgaccgag ccggtcgtgc tcgtcaacat cgcccgggag tgggatccag
      841 cactcacgga tcgcgacctg tacgagcgat cgcggcgata ctggcgctgc tcgccgcagc
      901 gccacccggc gaattatgcg atggtggtgg gtgacggcat catccgcgaa gtgtaccgga
      961 tcgacggatg gcacgaggtc gatatggccg ctgccgagct cgacccgggg cggaagggcg
     1021 cggccgagcc gctgccgcgg cacaccaggc gccaggaatt cgacgacgag atcgccctgc
     1081 acatgcggca ctatgtcggc aaatcagcgc gccatctgct cggccagaat ccgttcagat
     1141 ggctcaattg ctgacctgac cggacgatcg ccggcggtcc cggcccgatg gtcgggccgg
     1201 gaccgccgct cgcctagagc aggtcgagct gggccacgcc gagcgggacg tcgcgcgcgt
     1261 cgtcccagat atagacgtgg cggaactggc cgatgtcgac gccggccggc acgccgtaga
     1321 cctgggcacc ggtcgggctc gccagggggg ccaggacgac cgtggccccg gccgcatcgc
     1381 cgaggccgac cgtcgcaccc gcggcgttgt cgatgctgaa gtcggagccg aggctgacgg
     1441 catagccggc cgtcgtctgg aacaccgcga cctcgccgga ggtggtgcgg ccgctcgtgc
     1501 cggtgaagtc gccgacggca atctcctggc cggtgatccg gtgcgccgcc ggcgtcgtga
     1561 ccttggaccg ccacggagag tcgtccgccg tggttccgca ggccgccagg aaggcggctg
     1621 ccgccatgat cgtcagatgt cgcttcattg atccgtcctc attgaggccg cacccccttc
     1681 ggccgcgccc gggcgttatc ttgcccgcca cgaagccgca tgcctagccc gatggtacgc
     1741 atgcggcatc gcgagcccta cagtccagca ccgttgcgct cgccccgcac ctcgggcagg
     1801 ctcctagcca gggatgcggt cagcaggccg agcaggatcg ccgttcccat cgtccggcgc
     1861 gtcctccgcc acatgcatga gcaggtcgcc gggctggcag tcgagaaacg tgcagatccg
     1921 cgccagcgtc gcaaagcgca cccccttcac cttgccctgc ttgaagagcg agagattggc
     1981 ctcggtgatg ccgacgaagg ccgcgagatc gcaggaacgc gccttgcgcc gcgccagcat
     2041 cacgtcgagc gtcaccacga tcggcactgg gacaggctcc tagacgatct gcgcgtggtc
     2101 gtcggcgagc atggccgccc gttccagcac ctcgccgatc gcccagacca tgccgccgat
     2161 gagcacacct agcgcctggt cgctgccgag gctgatcacg agctggtgct ggccggggcc
     2221 ggcattggcg gtcagcacga ggccgagcac ggtcggcatg acgaggccga gtccggcggc
     2281 gagaccgacg cgaatgccca tgcgccggag cgccgccgcg gcatcgcgcg tcaggtacga
     2341 gccggcagcg aagccgcgaa agcagcgcca ggccgccagc agcgccgtgc tcaggatcag
     2401 gctcggcagc agcgccagaa aagccgccat cccgacctgc catggctcgg gcgcatgggt
     2461 gaggcgcgcg cccgccaccc gtgcgaactg cgccgacgga tcgcccgcca gccagaacca
     2521 gagcaccagc aagggcagga ccacggccag gaccagtgcc gcggtcgcca gccgcccgct
     2581 cagccgccgc agccgtgcgt cggcaccacc tcgttgcatc gcagcctcca ccatcgcggg
     2641 gggcaggcta gccatatgat ttctgtaaaa caataataaa tcattgttgg acgatgtcgc
     2701 gaggcggccg ccgccggtcg ggccgtgcgc gatcaccggg ccggcacggt cgcgcgaata
     2761 gctgcgctgc gggaggagaa tcccggctat ttctcgcctt cctgatcgcg tggttgtcac
     2821 gctgccgttc cgccaagtat agcagcctcc ggtcgggcct gtagctcagt ggttagagct
     2881 gaccgctcat aacggtctgg tcgcaggttc gaatcctgcc gggcccacca accgccgtgg
     2941 cggcgacgcg ccagcgcgac acggctgccg ggcggagctt gactagcgtg atggttgcgt
     3001 cgacacccag aatgatgttc gagccggctg ccgcttccgc caccgcccgc cctgccaccg
     3061 cccgccgcgt cgccacccgc cctgtcgaca tcggggacgc ggcgccgggc ggccgggacc
     3121 ggtcttgcgc tacctgatcg gcggcctcgc ggcgctggcc gtcgccatcg tcgccgccgt
     3181 cctcctggtg cagcatttct tcgacgagcg gctgctcgcc ctgcgcctgc agcaggcggt
     3241 ggccgacgcg accggtatcg agctggtcat cgagggcggg ctcgatctct cgctcctgcc
     3301 ccgcccggtg ctttcggtgc accggttgcg cctcgacgat gccgcgggcg acctcctgct
     3361 gaccgccgac cggctcgatc tcgacctcga gctgcgaccg ctcctggccg gcgagctcgg
     3421 cgtcaccgac gcccatctgg tgcgcccgga tctgcgtgtc atcggcaatc cggccactgc
     3481 cgccagccgc ctcgcggcct tcctcggcgc gagcctgccg ggcgaggccg tccagcgaat
     3541 ccgcatcgcc gacggcgcgc tgcaggtgac cggcgccgac ggcgccgccc cgctggtctg
     3601 gcgtggcatc gatgcggtgc tcaccggcga cagggtcacc ggcaccagcc acctcgcggc
     3661 cggcgccgcc ttcgccgggg tcgctgccgg cccgcgcctg gtgctcggtg gcgagctcgg
     3721 cagcgccgct ccgggacggc cccgggcgct ccgtctcgat ctggcgctcg aaggcacggc
     3781 gtcggcggtg ctcggctatc gcggtcagct ccgcccggat ggcgggccgg atcgccaggg
     3841 cgcgctcctc gacggccgcc tgacgctcga cctgccggac ccggcgggcc tgcgggactg
     3901 gctcgctctg gtcgccccgg cgctggcggc ctggccggtg cccgaagcac cgctcggcct
     3961 cgaggccgag ctcagcttcg cgccggccgc cgccgcggac ccgatgttcc tgcgcctcgg
     4021 cggcctggcg ctgaccgtgg ctggccagac cctggacggt gccctggatc tcgtcggcgg
     4081 tgaagcaccc gtgctcgacc tccagcttcg ggcagggcgg ctggagctgc cggacgactg
     4141 gcccgctgcc ggcgatccgc gccgcctcgc cgccgtgctg ccggccgacc tgcgcggcca
     4201 cctcgagctc gaggtcgacc agctcgactg gcgacggcgg agctttcgcc agatcgccat
     4261 cggcctcggc ctcgagggtg acggcgacgt gcgggtcgaa cgagccgtgg cggtgctgcc
     4321 gggaccgggc gacctcgact tcaccggccg tgtcgggccg ctcggcagcg ccaccggtca
     4381 ttggctgagc ggccgcctgc aggccgccct gcaggtcccg ggcgagcttc tggccctctt
     4441 cggcgagccg cccgccatgc tccggcaatc gacgacgctc gccctcgata ccgatctcga
     4501 ctggtcggcg gcggctctga ccctgcgcaa cgccgatctg agcctcgatg cgacgcgggt
     4561 cgagggcggc atcgcctggc ggccggcagt ggacggcaag cgcgcccagc tggcactgcg
     4621 cttgatgctc gaccgcctcg atctcggcga ggtcgtcgac gtccacgacc cccgggccac
     4681 gatcgacacg ctcgaggcga tcctcccggc caccgactac gccgtcgacc tcaagatcga
     4741 gcgggccagc ctcgacgagc ggcgcctcgg cgccctgacc ctgcgcctcg acagcgtcga
     4801 cggcgaggtg gcgatcagcc gggccagcct cgacggtctc gccggcagtg ccttgatgct
     4861 gtcgggcagg gtcacccccg cgagccgggc gttcgacctc gatctcgcct tcgacatcgc
     4921 ctcgctcggc cggctcctgc gcctcgtcga ctaccccgcg ccgccggcgc tcgccctgct
     4981 cgggccggtc aatctgcgcg ccagcctcac cggcgacgcc gccagcacca ccatcgacgc
     5041 caccctcgac ggcgacagct tcggcgcgtc cgccagtggt gtgctcgacg actggacgac
     5101 ggcccgcctc ggcggcacgc tggccctcac gctcggcgcc gccgatgccg cgggcgtcct
     5161 ccgccagctc ggcggcgtgc cgatcacggc cgccgtcttc accggcccgc tcgatgccga
     5221 cctgcagctc ttgctgactg atggccggcc gaccgcctgg aatctcgaag caagcctcgg
     5281 cgggctggat ctcgacctcg tccgggagcc tggcacggaa ggggaggcgg agcgcctgga
     5341 tctggcgatc ggaccgctct cgccggcggc ggcagccgtc gtctacgagc tcgccacgcc
     5401 gctcctccag ctcgtcccgg gcccgcccgg gcgctggctg ggcttctggc ccgaccagag
     5461 cctgcgcttc gactggctcg acggcgagcc gcgcgcgatc aggatcgagc ttgtgcccga
     5521 ggcgagcccg gcggcacccg tcgtcatcga ggccgatctc gccgatggcg tgctcgccgt
     5581 gccggccttc gcctgggaaa acgacggcat gcggctcgcc ggcagcatgg cgctcgagca
     5641 tcgccatgcc ggtgctgcgg cagcgctcag cctcgacctg gcgctcgacg gtgccacggc
     5701 gacccggctc ggcgagctgc tcgacctgcc ggcgggcgtc acgggcgagg tcggcctcga
     5761 ggcccgggtg acgagcctcg gccgcagcgt gcgcaccctg atcggcaatg cctcgggcga
     5821 agccgcgatc gcgctcgaga acggagcgat cggcgcggtc cccgtcgacc gcctcgccgg
     5881 cgatctcatg ctggagcggg gcgtcctgcg cccggtcggc gacggcatcc cattcgctgg
     5941 accggagggc agcggctcgg tcaccggagc cgtcgatcta ttggcctggc tcgccgatct
     6001 cgagctcgag ctgacgcccg ccgcccccgg cgcccccgcc cgccaccagc gcctgctcga
     6061 tggcctcagg acacccgccc cctcggacta ggccctcagt cgctcgggcc gagcatccgc
     6121 tccggccgca ccagcgcgtc gaactcggcc tcgtcgagaa ggcccagggc cagggcggat
     6181 tccttcagcg tggtgccctc ggcatgcgcc ttcttcgcca ccttggcggc gttgtcgtag
     6241 ccgatcctcg ggttgagcgc cgtcaccagc atcaggctct cgtggagcag cttctcgatc
     6301 cgcgcctcgt cggcggtgat cccgacgacg cagttgtcgg cgaagctgac cgaggcatcg
     6361 gcgatcagcc ggatcgactg cagcacgttg tagatgatca ccggcttgaa gacattgagc
     6421 tcgaaatggc cggtggcgcc gccgaccgtc acggccacgt ggttgcccat cacctgcgcg
     6481 cagacctggg tcatggcctc gcactgggtc gggttgacct tgcccggcat gatcgaggag
     6541 ccgggctcgt tctccggcag cttgagctcg ccgagcccgc agcgcgggcc ggagccgagc
     6601 aggcggatgt cgttggcgat cttcatcagg ctgacggcga cggtgttcag cgcacccgag
     6661 agctcgacca gcgcgtcatg cgcggccagc gcctcgaact tgttgggggc cgtctcgaag
     6721 gcgagcccgg tgatccggct ggccgccgcg gcgaaggcca cgtcgaagcc cttcttgctg
     6781 ttgagcccgg tgccgaccgc cgtgccgccc tgggcgagct ggctgatccg gggcagggtc
     6841 ccgcgcaccc gctcgatgcc gtaggcgagc tgctgcacgt agccgccgaa ctcctggccg
     6901 agcgtcaggg gcgtcgcgtc ctggagatgc gtgcggccga tcttgacgat cttctcgaag
     6961 gccgcggcct tggcggcgag cgcgtccttg agatggccga gcgccggcag cagccgctcg
     7021 tgcacctcct gggtggccgc gatgtgcatc gccgtcggga aggtgtcgtt cgacgactgg
     7081 ccgcgattga cgtggtcgtt ggggtggacc agcgacttct gcccgcgctc gccgccgagc
     7141 agctcggtgg cgcggttggc gatcacctcg ttggcgttca tgttcgactg cgtgccggag
     7201 ccggtctgcc agaccaccag cgggaaatgg tcgtcgagct tgccatcgat cacctcctgg
     7261 gcggcagcac cgatcgcctc ggccagccgg ccgtcgagcg agccctgcgc ctggttgacc
     7321 tcggccgccg cccgcttgac cacacccaag gcgcgcacca gcggcagggg catccgctcg
     7381 ccgccgatcc tgaagttctg caggctgcgc tgggtctgcg ccccccagta gcgggacgcc
     7441 tcgacctcga tcggcccgaa cgtgtccgtc tcgatcctga cgttgctggt catccacccc
     7501 tccgctcgac cgccttcgct ggccagccgc atggcccgcc acccggcgcc ttgtcaatcg
     7561 cgttgccacg ccaggcggca gatgccgggc cggctatttc ttgcggaagc gatcgagggt
     7621 gacgatctcg gcgccaccct cattggacgg gggctccgcc ggcgcttcgt cctcggaggc
     7681 caccgggcgc ggcttggcgc cggtcttggc ggtgagcggc agggaaaccg gctcctcgcc
     7741 gtccttctcc gcacccaggg tgaactgcag cccgaactcg acgccgggat cggcgaagac
     7801 ccgcaacgcc tcgaacggca cgaccagccg ctccggctga tcgttgaagc tcagcgtcac
     7861 ggcgaacttg tcgtcctcca cctccagatc ccagaactgg tgctgcagga cgatggtcat
     7921 ctcgttcgga tagcggctgc ggaggaaatc ggggatctcg acgcccggca tgtcggtccg
     7981 gaaggtgacg tagaaatggt gcggcgacgg cacgtcgccg gccgcgaaac gccgcagcac
     8041 atcacgcacc accgtgcgca atgccgtctc gacgagctgt ccgtaatcga tggtctgccg
     8101 ccgcatcggc cttcacacgg ttcgggcacc tgccggggcg gcgtggtgcg tctccctaac
     8161 cctagccacg gatggcccct cttgtgaaga gcggcccgca gaagagtggg gggcttctgt
     8221 tgcccggcgc cccccgggcc gcgccagggt gccgaagctc c
//
LOCUS       DAOTFI010000219         8254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10429_length_8254_cov_7.819002, whole genome shotgun sequence.
ACCESSION   DAOTFI010000219 DAOTFI010000000
VERSION     DAOTFI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10429_length_8254_cov_7.819002"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..577)
                     /locus_tag="P5558_18850"
     CDS             complement(2..577)
                     /locus_tag="P5558_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase"
                     /protein_id="HRY26434.1"
                     /translation="MAAWIEMLPLEGAEGDLALAYAAARTPHGTVDNVMRAHSLRPHT
                     MLGHMALYKSVLHHPANTLPLWFLEVVAAVVSHANACAYSYGHHWTNARRLIADPPRA
                     TAIEAAIKARRLADAFAGRELALLAYAERLTVDVAGIDEATFRACVDAGASHGEILEV
                     NQVTAYFNYSNRLLNGLGVTTEGDRLGYYDE"
     gene            complement(661..2331)
                     /locus_tag="P5558_18855"
     CDS             complement(661..2331)
                     /locus_tag="P5558_18855"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HRY26435.1"
                     /translation="MQRKGSDGIETHRNRGGRWRGATQGPLLIGFGTLNALASLGLYL
                     GQGLLVPLLPCLALSLAAIGIGGWQHRRAAGKHASPGALDLLVRPGAATGPQPSTPLV
                     PLYRQPAAHRRRRVLDLLTDLPGRDSVAVSLRRAIEEAGDSEQRIALVAFDIDRMKDI
                     NGVHGYAVGDQLLRIVARRLEGIARDGVFRLDGDRFVLLRRGLAGVADAELCAVAALD
                     RLGRAVRLGAGEKHEMTPSASVGIALYPDHGHDFEALLRCAEMAVDDAKRAGGRRCRV
                     FDRGMVAAMQFRNEIERDLAEAIEEGALNLHYQPQLDLAMGRITSVEALMRWTHPTRG
                     VIPPTTFIPIAEASGLIKPMGTWLIHEACRTARRWQDMGLEIGMAINISAAQLRQQDL
                     PEIVTRALVENGLSPDRLELELTESLFVDPSELMMRRSLDRLAEMGIRLAIDDFGTGY
                     SSLAYLKRLPVQKIKIDKSFTQGIGREEVDEALVRVIIHLARTFGKLVLAEGVETEAQ
                     HHFLADEGCDAAQGYLFAKPMPLALCTEHLLAQADRHPMPRTARAGVG"
     gene            2738..4432
                     /locus_tag="P5558_18860"
     CDS             2738..4432
                     /locus_tag="P5558_18860"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRY26436.1"
                     /translation="MAEAPSDIAIARAAAMRPIQDVAASLGIPEAAIHAFGRHIGKVD
                     LGWIDGIRDRPEGKLVLVTAISPTPAGEGKTTTTVGLGDGLNRIGRQAMICLREPSLG
                     PCFGMKGGAAGGGYAQVVPMEQINLHFTGDFHAIGSAHNLLAALLDNHVYWGNELAID
                     ERRIALRRAIDMNDRSLRSIVSSLGGVANGFPREDGFDITVASEVMAVFCLARDLADL
                     EARLGRMVVAQTRERRPVTAAELEAPGAMTVLLKDAFMPNLVQTLEGNPAFVHGGPFA
                     NIAHGCNSAIATRTALRLADFVVTEAGFGADLGAEKFFDIKCRKAGLTPAVAVVVATV
                     RALKMHGGVAKEDLGRENLEALRHGLANLARHVHNIGKFGVRAVVAINQFVSDTAAEH
                     ELIRAFAKDELGVEAFVCSHWAHGGAGTEALAHHVAALCDAPEAADRPGFRPLYPDEM
                     PLWTKMRTIATEIYGAADIIADKAVRARFRELDEGGYGRFPICVAKTQYSFSTDPNLK
                     GAPSGHVVPIREVRLSGGAEFLVVVCGEIMTMPGLPRKPAAHQIRIDSDGLIDGLF"
     gene            complement(4572..5954)
                     /locus_tag="P5558_18865"
     CDS             complement(4572..5954)
                     /locus_tag="P5558_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY26437.1"
                     /translation="MAEDRRGLEHTSTSDEAVGLFDRAVDHYLEYRLDTPDLLKQAKE
                     ADPAFVMPHILKASMLRLMGSNSVEAAIATEVAALDALDVARNPREALHETALRHWLK
                     GDLTAATRAWEAIVVEWPFDLVALRHLHFQSFWLGEQQAMRNAVAGALPAWSPELPGY
                     GFVQGMLAFGMEEAGDYARAEAVGRDAVERNPDDLWAIHAVAHVMEMQGRLQDGRTWL
                     DYPADRWADRGPVKNHVWWHAALFAVESGDFDAALALYDTHVAPGERRISTDMMNAPS
                     LLARLELAGQNVGDRWDELALWAEGWVDDHVIAFTDSHTMIPLARTGRNEAADRLLAS
                     LERFAQEPRNDAARRAEPFLLPLARGIRAFYDGRPGETVELLQPIRDRLQPIGGSHAQ
                     RDIFHQLLLEAAIRDGRWPLARALASERITLRPTSHLNWLKLAELFEAAGDPDASKRA
                     RDEAAARLAA"
     gene            complement(6026..6946)
                     /locus_tag="P5558_18870"
     CDS             complement(6026..6946)
                     /locus_tag="P5558_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26438.1"
                     /translation="MAGQGGRMTLREWSLLGLLAVLWGGSFVFAEIGLRELAPPWLVL
                     SRLAFAALALQLVMRLAGTTIAMSPALFGRFLVMGCLNNVVPFSLIVWSQTAIEGSLA
                     AILNATTPFWMVLLAHFLTADERATPQKFTGIAIGWGGVVVMLGPGLLAGLGQDLLPQ
                     LAVILATLFYAFSGLYARRLRDVPPVAGAAWQLTASTTVMAPLVLLTQPFPALQTLAP
                     LTWAAVLALALASTAFAYVLFFRILRTAGPTNLMLVTLLIPVTAVILGGLVLGERLSG
                     EQVLGMALILAGLAVIDGRVLRPIGALLGR"
     gene            complement(7041..7454)
                     /locus_tag="P5558_18875"
     CDS             complement(7041..7454)
                     /locus_tag="P5558_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26439.1"
                     /translation="MLAALPLLVFVVGVYHLIVLFGIAGLGGIASVVLSLPLVSGATL
                     ELTMGDLLVLAGLVLLYIEIFKATRTSTASIVDHLLSMALFVVCLVEVIVLPGFGTAT
                     FVILTLMTAIDVVAGFTVTISGARRDIGLEDGIRR"
     gene            7542..>8254
                     /locus_tag="P5558_18880"
     CDS             7542..>8254
                     /locus_tag="P5558_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26440.1"
                     /translation="MNKIDLAGRRAVVTGGAQGIGRAIAERLLASEASVALWDMDSDL
                     TAATAKELGGQGTVQAVTVDVTDFASVERAVEATTKELGGIDILVCNAGIAGPSFKLW
                     EYPNDAWQQVIDIDITGVFNCMKAVVPSMIAQNYGRICSTASIAGKEGNPNAGPYSAA
                     KAAVIALTKSLGKELAEYDIAVNCITPAAARTRIFDQISQQHIDYMLSKIPRGRFLQV
                     EEAAAMVAWIVSAENSFTTG"
BASE COUNT         1274 a         2718 c         3066 g         1196 t
ORIGIN      
        1 ctcactcgtc gtagtagccg agccggtcgc cttccgtggt gacgccgagg ccgttcaaga
       61 ggcggttgga gtagttgaaa taggcggtga cctggttgac ctcgaggatc tcgccatggc
      121 tggcgccggc gtcgacgcag gcccggaacg tggcctcgtc gatgccggca acgtcgaccg
      181 tgagccgctc cgcgtaggcc agaagcgcga gctccctgcc ggcgaaggcg tcggccagcc
      241 ggcgcgcctt gatcgccgcc tcgatcgccg tggcgcgcgg cggatcggcg atcaggcggc
      301 gcgcgttggt ccagtggtgg ccgtaggaat aggcgcaggc attggcgtgg ctgacgaccg
      361 cggcgaccac ctcgaggaac cagagcggca gcgtgttcgc cgggtgatgc agcacgctct
      421 tgtagagcgc catgtggccg agcatggtgt gcggccggag cgaatgggcg cgcatcacgt
      481 tgtcgaccgt gccatgcggc gtgcgggccg cggcgtaggc cagggcgagg tcgccctcgg
      541 caccctcgag gggcagcatc tcgatccagg ccgccattcc gctcgctccc ggctggtgcg
      601 gccaaggccg gccgcgtggc tatcctgccg ggtttcgcgc gctccggcca gatctcgtgg
      661 tcagccgacc ccggcacgcg ccgtgcgggg catcgggtgc cggtccgcct gggcgagcag
      721 atgctcggtg cagagggcga ggggcatcgg cttggcgaag agatagccct gcgccgcgtc
      781 gcagccctcg tcggcgagga agtggtgctg cgcctcggtc tcgacgcctt cggcgagcac
      841 cagcttgccg aaggtgcggg cgaggtggat gatcacccgg accagggcct cgtcgacctc
      901 ctcgcggccg atgccctggg tgaacgactt gtcgatcttg atcttctgca ccggcagccg
      961 cttgagatag gcgagcgacg agtagcccgt gccgaaatcg tcgatggcga ggcggatgcc
     1021 catctcggcg aggcgatcga gcgagcggcg catcatcagc tccgagggat cgacgaacag
     1081 gctctcggtc agctcgagct ccagccggtc ggggctcagg ccgttctcga cgagggcgcg
     1141 ggtgacgatc tccggcaggt cctgctggcg tagctgggcc gcggagatgt tgatcgccat
     1201 gccgatctcg aggcccatgt cctgccagcg gcgcgccgtg cggcaggcct cgtggatcag
     1261 ccaggtgccc atgggcttga tcaggcccga cgcctcggcg atcgggatga aggtggtggg
     1321 cgggatcacg ccccgggtcg ggtgggtcca gcgcatgagc gcctcgacgc tggtgatcct
     1381 ccccatggcg aggtcgagct gcggctggta atgcagattg agggcaccct cctcgatggc
     1441 ctcggcgagg tcccgctcga tctcgttgcg gaactgcatg gccgcgacca tgccgcggtc
     1501 gaagacccgg cagcggcgcc cgccggcgcg cttggcatcg tcgacggcca tctcggcgca
     1561 gcgcagcagc gcctcgaaat cgtggccgtg gtccggatag agggcgatgc cgacgctcgc
     1621 cgagggggtc atctcgtgct tctcgccggc gccgaggcga acggcccggc cgagacgatc
     1681 gagggcggcg acggcgcaga gctcggcgtc ggcgacgccg gcgagcccgc ggcgcaggag
     1741 gacgaagcga tcgccgtcga ggcggaagac gccgtcgcgc gcgatgccct cgagccgcct
     1801 tgccacgatg cgcaggagct ggtcgcccac ggcatagccg tgcacgccgt tgatgtcctt
     1861 catgcggtcg atgtcgaagg cgacgagcgc tatccgctgc tcgctgtcgc cggcttcctc
     1921 gatcgcccgg cggagggaga cggcgaccga gtcgcggccc ggcaggtcgg tcaggaggtc
     1981 cagcacccgg cgccggcgat gcgcggcggg ctggcggtag agcgggacca ggggtgtgga
     2041 gggctgcggg ccggtggccg caccgggccg caccaagagg tcgagagcgc cgggcgaggc
     2101 gtgctttccg gccgctcggc gatgctgcca gccaccgatg ccgatcgcgg cgagcgagag
     2161 ggcgaggcag ggcagcaggg gcacgagcag cccctggccg agatagaggc cgagcgaggc
     2221 cagggcgttg agcgtgccga agccgatcag cagcggaccc tgcgtggccc cacgccagcg
     2281 accgccgcga ttgcggtggg tctcgatccc gtcgctgccc ttgcgctgca tcgccgccat
     2341 ccagcactca atgttcagtc gtgccgtggt ggcatcgcgt tgacggccag gcggtccggc
     2401 ctcggtggaa ggcagtgtcg ctcggtggtg gttggcaata cggtctctcg ccagagggct
     2461 ttattgctga tcgcggatcc gccggcggca tgctgcgcaa tgaaccgatg attttcaagc
     2521 ttttcatttg atcagcttga ggttgagagg ttcactgtgc ccttgcgcgc agggcgacgg
     2581 cgaagttgcg gcggctaccg gccgaatgta ggcaggacca atgcaggtgt cgccgcgctc
     2641 gtgcaattgc gtgacgcgtc caacactagc cagccggatg ccggcgcagt tgtctgcgcg
     2701 catcgtcaac agcgttttta tcagggagag gcagaacatg gcagaggccc cgagcgacat
     2761 cgccattgca cgggcagccg ccatgcgccc catccaggac gtcgccgcgt ccctcggcat
     2821 tcccgaggcc gccatccacg ccttcggccg gcacatcggc aaggtcgacc tcggctggat
     2881 cgacggcatc cgggaccggc ccgaagggaa gctggtcctg gtcaccgcga tcagcccgac
     2941 gccggccggg gagggcaaga cgacgaccac ggtgggcctc ggcgacgggc tcaaccggat
     3001 cggcaggcag gccatgatct gcctgcgcga gccctcgctc gggccctgct tcgggatgaa
     3061 gggcggggcg gccggcggtg gctatgccca ggtcgtgccg atggagcaga tcaacctgca
     3121 cttcaccggc gacttccatg ccatcggctc ggcgcacaac cttctggcgg cgctgctgga
     3181 caatcacgtc tactggggca acgagctggc catcgacgag cggcgcatcg cactccggcg
     3241 ggcgatcgac atgaacgacc gctcgctccg ctccatcgtc tccagcctcg gcggggtcgc
     3301 caacgggttt ccgcgcgagg acggcttcga catcaccgtg gcgtcggagg tgatggcggt
     3361 cttctgcctc gcccgcgacc tcgccgacct cgaggcacgg ctcggccgga tggtggtggc
     3421 gcagacccgc gagcggcggc ccgtcaccgc cgccgagctc gaggcgccgg gtgccatgac
     3481 cgtgctgctc aaggatgcgt tcatgcccaa tctggtgcag acgctagagg gcaatccggc
     3541 cttcgtgcat ggcggtccct tcgccaatat cgcgcatggc tgcaactcgg cgatcgccac
     3601 ccgcaccgcc ctcaggctcg ccgacttcgt cgtcaccgag gccggcttcg gcgccgatct
     3661 cggcgccgag aagttcttcg acatcaagtg ccgcaaggcc gggctgacgc cggcggtcgc
     3721 ggtggtcgtc gccacggtcc gtgccctgaa gatgcacggc ggtgtcgcca aggaggatct
     3781 cggacgggag aacctggaag ccctccgcca cggcctggcc aacctcgcgc gccacgtcca
     3841 caatatcggc aagttcggcg tgcgggccgt cgtcgccatc aaccagttcg tcagcgacac
     3901 cgcggccgag cacgagctga tccgtgcgtt cgccaaggac gagctcggcg tcgaggcctt
     3961 cgtctgctcg cactgggcgc atggcggtgc cggcaccgag gcgctggcgc accatgtggc
     4021 ggcgctctgc gatgcgcccg aggcggcgga ccggccgggc tttcgcccgc tctatccgga
     4081 cgagatgccg ctctggacca agatgcggac catcgccacc gagatctacg gggcggccga
     4141 catcatcgcc gacaaggcgg tgcgcgcccg cttccgggag ctcgacgagg gcggctacgg
     4201 gcggtttccg atctgcgtgg cgaagaccca gtacagcttc tccaccgacc ccaacctgaa
     4261 gggggcgcct tcgggccatg tcgtgccgat ccgcgaggtc aggctctccg gcggggccga
     4321 gttcctggtc gtcgtctgtg gcgagatcat gaccatgccg ggcctgccgc gaaagccggc
     4381 ggcgcaccag atcaggatcg acagcgacgg gctgatcgac gggcttttct agcgcgcctt
     4441 cctcgtaacc gtatacttcg tcaggctgcg gacgatcggt gctcacgtag atggaccacg
     4501 ctgcgcgccg atcgccccat cagggttggg ggccttcctc gtctacggtt acggggaagg
     4561 cgctctagcc gctaggccgc gagccgggcc gccgcctcgt cgcgggcgcg cttgctggcg
     4621 tcggggtcgc cggccgcctc gaagagctcg gcgagcttca gccagttgag atggctggtg
     4681 ggccggagcg tgatccgctc gctggcgagg gcgcgggcga gcggccagcg gccgtcccgg
     4741 atcgcggctt ccagcaggag ctggtggaag atgtcgcgct gggcgtggct gccgccgatc
     4801 ggctggagcc ggtcgcggat cggctgcagg agctcgacgg tctcgcccgg gcggccgtcg
     4861 tagaaggcgc ggatgccgcg agccagcggc aggaggaagg gctcggcccg ccgggccgcg
     4921 tcgttcctgg gctcctgggc gaagcgctcg agcgaggcga gcagccggtc ggcggcctcg
     4981 ttgcgcccgg tgcgggccag cgggatcatg gtgtgcgagt cggtgaaggc gatgacgtgg
     5041 tcgtcgaccc agccctcggc ccagagggcg agctcatccc agcggtcgcc gacattttgg
     5101 cccgccagct cgaggcgggc gaggagcgag ggggcgttca tcatgtcggt cgagatgcgc
     5161 cgctcgccgg gtgcgacatg cgtgtcgtag agcgccagcg ccgcgtcgaa atcgccgctc
     5221 tcgacggcga agagggcggc atgccaccag acatggttct tgaccgggcc gcgatccgcc
     5281 cagcggtcgg ccgggtagtc gagccaggtg cggccgtcct ggagccggcc ctgcatctcc
     5341 atgacgtgcg ccacggcgtg gatggcccag aggtcgtccg ggttgcgctc caccgcgtcc
     5401 cggcccacgg cctcggcgcg ggcatagtcg ccggcctcct ccatcccgaa ggcgagcatg
     5461 ccctggacga agccgtagcc ggggagctcg ggcgaccagg ccggcagggc gccggccacg
     5521 gcgttgcgca tggcctgctg ctcgccgagc cagaaggact ggaaatgcag gtgccgcagc
     5581 gccacgagat cgaagggcca ttcgaccacg atggcctccc aggcccgggt ggcggcggtg
     5641 aggtcgccct tgagccagtg gcggagcgcg gtctcgtgca gcgcctcgcg ggggttgcgg
     5701 gcgacgtcga gcgcatcgag cgccgcgacc tcggtggcga tcgccgcctc gacgctgttg
     5761 gagcccatga ggcggagcat cgacgccttg aggatgtgcg gcatgacgaa ggcggggtcg
     5821 gcttccttcg cctgcttcag caggtcgggg gtgtccagcc ggtattcgag atagtggtcg
     5881 accgcccggt cgaagaggcc taccgcctcg tcgctggtgc tggtgtgctc gagtccccgt
     5941 cggtcttccg ccatgcggcc gtctccctgc gtgctggcga gcaaaattgt gcatgggcgg
     6001 ccggctggca aggccggcgg gtgtgtcagc ggccgaggag cgccccgatc ggtcgcagga
     6061 cgcggccgtc gatcaccgcg agcccggcga ggatcagcgc catgccgagc acctgctcgc
     6121 cgctcaggcg ctcgcccagg acgaggccgc cgaggatcac ggcggtgacc gggatgagca
     6181 gggtgacgag catcaggttg gtgggtccgg ccgtgcgcag gatgcggaag aagagcacat
     6241 aggcgaaggc ggtgctggcc agcgccaaag cgaggacggc cgcccaggtc aggggggcga
     6301 gggtctggag cgcggggaag ggctgggtga gcagcacgag cggcgccatg acggtggtgc
     6361 tggcggtgag ctgccaggcg gcgccggcga ccggcggcac gtccctgagg cggcgggcgt
     6421 agagcccgct gaaggcatag aagagcgtcg ccaggatgac ggcgagctgc ggcaggaggt
     6481 cctggccgag cccggcgagc aggcccgggc cgagcatcac cacgaccccg ccccagccga
     6541 tggcgatgcc ggtgaatttc tgcggcgtcg cccgctcgtc ggcggtcagg aaatgggcga
     6601 gcagcaccat ccagaagggc gtggtggcat tcaggatcgc cgccaggctg ccctcgatcg
     6661 ccgtctggct ccagacgatc agcgagaacg gcacgacatt gttgaggcag cccatgacga
     6721 ggaagcggcc gaagagggct gggctcatcg cgatcgtcgt gccggcgagc cgcatcacga
     6781 gctgcagggc gagcgccgcg aaggcgagcc gcgacagcac gagccagggc ggcgccagct
     6841 cgcgcaggcc gatctccgcg aagacgaacg agccgcccca gagcaccgcg aggagcccga
     6901 gcaggctcca ttcgcgcagg gtcatccggc cgccctggcc cgccatggca tcactccgtt
     6961 gcagctatgc gctgggcatg ccccaggccc gggcggcgga cgctccgatt cttgcgcccg
     7021 aagctcgccc ggacaggctc ctagcggcgg atgccgtcct cgaggccgat gtcgcgccgg
     7081 gcgccgctga tggtcaccgt gaagccggcg acgacgtcga tggcggtcat cagggtcagg
     7141 atgacgaagg tggcggtgcc gaagccgggc aggacgatga cctcgacgag gcagacgacg
     7201 aagagcgcca tggagagcag gtggtcgacg atcgacgccg tgctggtgcg ggtcgccttg
     7261 aagatctcga tatagagcag cacgaggccg gcgagcacca ggaggtcgcc catggtgagc
     7321 tcgagcgtgg cgccggagac cagcggcagc gacaggacga cgctggcgat gccgccgagg
     7381 ccggcgatgc cgaagagcac gatcaggtgg tagacgccga cgacgaagac gagcagcggc
     7441 agggcggcga gcatccgggt tcctccaagc gtgccgccgt gactaccgca gccgcggcaa
     7501 accgtctatc gagacgggca ttcatcgagg ggagaacgga cgtgaacaag atcgatctcg
     7561 ccggccgacg ggcggtcgtc accggcggtg cccagggcat cggccgggcc atcgccgagc
     7621 ggctcctggc ctccgaggcg agcgtggcgc tctgggacat ggacagcgac ctcacggcgg
     7681 ccacggcgaa ggagctcggc gggcagggca ccgtgcaggc ggtgacggtg gacgtcaccg
     7741 acttcgcctc ggtcgagcgg gcggtcgagg cgaccacgaa agagcttggc gggatcgaca
     7801 tcctggtctg caatgccggc atcgccgggc cttccttcaa gctctgggag tacccgaacg
     7861 acgcctggca gcaggtgatc gacatcgaca tcaccggcgt cttcaactgc atgaaggcgg
     7921 tggtgccctc gatgatcgcc cagaactacg gccgcatctg ctcgaccgcc tcgatcgcgg
     7981 gcaaggaggg caaccccaat gccggcccct acagcgccgc caaggcggcg gtgatcgcgc
     8041 tcaccaagtc gctcggcaag gagctcgccg agtacgacat cgcggtcaac tgcatcaccc
     8101 cggcggccgc caggacgcgc atcttcgacc agatcagcca gcagcatatc gactacatgc
     8161 tctcgaagat cccgcgcggc cgctttctcc aggtcgagga ggcggcggcg atggtcgcct
     8221 ggatcgtctc ggccgagaac tcgttcacga ccgg
//
LOCUS       DAOTFI010000220         8171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10572_length_8171_cov_6.313208, whole genome shotgun sequence.
ACCESSION   DAOTFI010000220 DAOTFI010000000
VERSION     DAOTFI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10572_length_8171_cov_6.313208"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="P5558_18885"
     CDS             <1..965
                     /locus_tag="P5558_18885"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY26441.1"
                     /translation="ARPPEAVDERPERRRRGGDKPFVLFVSYPSPHPPFSVPAEFWEG
                     IDEAGVRLPTGWRPEERSEHPADRHLRHIMDTGPLIDEAQMRRIQAGYLALIAHLDTE
                     IGKLLEGLEEAGLAQDTRVLYTSDHGDLAGQNGLLGKSCLYEGSIGVPLVMAGPGIPE
                     GRRVAQIAGHVDLYPTIAEAVGGRLEAADDDLPGRSLFPALQGEEAAVQGFAEYHAAG
                     SKAGSFMLRDGDLKLIYHVGMPSQLFELEADPDELSDLGPDHPEVARLEALLRRICDP
                     EAVDARAKADQRAKVEFWGGREAVEQEGLLVYTPPPGGKAEIVA"
     gene            962..2437
                     /locus_tag="P5558_18890"
     CDS             962..2437
                     /locus_tag="P5558_18890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY26442.1"
                     /translation="MTKRPNILWYCTDQQRWDTIRRLGNPHINTPRLDAFCESGVAFD
                     NAYCQSPICTPSRASMMTGRYPASTHVHRNGNAHFPAGETVVTKLLADAGYDCGLIGK
                     FHLTAAKGHEKRFDDGYRLWEWSHHPKPDLDVEHHAYHRWLVEKGVDPHALHASIEPF
                     CGAGMPEELHQTTWITERAISFINEERDGPWMLSLNPFDPHPPFDPPKAYLDRYDPEK
                     LPPAAFRPHDIERQKAFAGITQQTPEAIDPTGPMPKVELTAEERLTTGYKPPKQYNDR
                     IVKAAYYAMIELIDTQFGRLIDALEASGQLDDTIIVFHSDHGETLGDHGLLYKGCRFF
                     EGLVHVPMIWSWRGKTRTDLRTDALVELVDIAPTLLEAAGLPVPANMQGRSLWPILTG
                     EAAPQHHKDHVISEFNDALGSATGEGPSHGSMYFDGRYKHILYQGTGLGELYDLQADP
                     DEFDNRFNDPALAELRLELMHKHCDALLASSDAGVERVSIY"
     gene            2552..4093
                     /locus_tag="P5558_18895"
     CDS             2552..4093
                     /locus_tag="P5558_18895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY26443.1"
                     /translation="MGKRPNILLITSDQQRGDCLGCEGRGVKTPHIDLLAEQGTRFAT
                     CITPNNVCQPTRASILTGLLPRTHGVADNGLDLPPATGEQGFAGRLSRAGYRSALIGK
                     PHFATSHTFEPTGTPECRGSMHLWGEDWNGPYMGFDHVELVIEGHNQWPPERPPSGQH
                     YERWYYQDGLGDLKNRLFSTNVGPDTHGAPQTWHSGLPVAWHNSSWVGDRTVAYVNDH
                     QANHSDQPFVLWASFPDPHHPFDCPVPWSLLHDPAEVDLPEHRTLDLDRRPWWHRAAL
                     EGKPQIREDLAKIREQYSRVRQLSDAELREIIANTYGMLALIDHNVGRIMAELHRLGI
                     ADDTLVVYASDHGDWLGDHGLLLKGPMAYEGLMRVPLVMRGPGVPVGKVIEDPVSTLD
                     LGATFLDTAGVERPAHWHSRSLLPVLRGDEHREFALNEWELNASRCGVELKLRTVRTR
                     THRLTLELVSGAGELYDLVADPHEMNNLFDDPACAAVQGALSAMIDSRPDDALEPPLT
                     PVGMA"
     gene            4165..5199
                     /locus_tag="P5558_18900"
     CDS             4165..5199
                     /locus_tag="P5558_18900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRY26444.1"
                     /translation="MSFTRRHTLGIGAGLAGSAFLPRLAMAQEWPTRPITIAMAAPAG
                     GATDLGTRAVTELMRQPLGARAIAFQDMSGAGGVQAMDYILQQPATGHFWLGANDVIE
                     AYPSLGRIDYDWRDFDFWMSGGTSCAIVVPGNSPYESFDQWVNAVMEKPGEVAIASTP
                     SGSLWSNAAVYLQKQMGLDFRLANYAGGGPTVRAVLAGETSCASVGVTPTVNFLKSGE
                     LRALAAWTPEAWEVAGTTIPSILDYVDDPILEATLPWTNIHGIAVRKGVDEAILEKID
                     AAFATAVNDPSMVEVYDSKAFFPFRAPRQVANDMMGKRTALQAYIIEVILGTAVKTRD
                     ELGIAKIEDS"
     gene            5255..5779
                     /locus_tag="P5558_18905"
     CDS             5255..5779
                     /locus_tag="P5558_18905"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY26445.1"
                     /translation="MDQSGTGPARPEPARRKAFYASPEKAAPDFVTGVVLFLLSLYVL
                     VTSYRMPHFAGSGWLGSPGLTPGLIALVLLLLSLALMFRARDFRLRLGPLQPRIEGLR
                     AGACFAMIFVYILVTPLIGYVPATFALLFVFQTAYARKLSVRYVLIWSIGLSALLTFI
                     LWYVFGEIFFVPLP"
     gene            5822..7333
                     /locus_tag="P5558_18910"
     CDS             5822..7333
                     /locus_tag="P5558_18910"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY26446.1"
                     /translation="MLEEVGRLLLDPWAIFTIFWSMTLGIIMGAMPGLTGPMAMALLL
                     GIAYTMQTEYSILAMMLIYMGGVYGGSLSAILLNIPGAPASAATAIDGYQLALQGKAG
                     RAIALTTIASCIGTLIAIAFLAFLTPWLVKVSLLFTSWELLLLVLLGVMMAGSLTGDD
                     PIKGWIAGMVGLLIAQVGLDDLHMHPRFSFGSHYLEAGVGLVAAIVGLFGISQVVVAM
                     RDRDIHLEPVTHKIGRIMPTLEDLKGRVRVVLQSGVIGSIVGILPGLGADMGAWISYV
                     LAKRTSRKPELYGKGSTEGVIAAETGNNAVVPSSLIPVIALGLPGSGGAAIIMAALFV
                     HGLTPGPMFLFENLDLFEYMVIGLLFGVVAMLIVGLALAHVIIHVLLIPRENIMAVVI
                     AMGVMGAYASKLQYGDVWMMLAFGVLAYVMRRLDFPLAPMVLGIVLGSLLDEFLRNAM
                     IIGDGDLSPLVTRPIALGLLIVLVLTLVGSLPVLRRSVSRLIAGARRPAAQAE"
     gene            7330..>8171
                     /locus_tag="P5558_18915"
     CDS             7330..>8171
                     /locus_tag="P5558_18915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRY26447.1"
                     /translation="MTSLARRLRRKGTRRPAARAVPAVVVPAGQPDLDTMVRLEGGEF
                     RMGSDRHYPEEAPSRRVRVDAFWIDPYPVTNADFARFVAATGYVTEAERTIDPALYPG
                     IDPGLLKPGALVFRPTQGPVDLKDFRQWWEYVPGACWRRPGGAGADALADHPVVHVTI
                     GDALAYARWCGKTLPTEAMWEFAARGGLEDADYCWGNEFEPEGRSMAKTWHGRFPYEN
                     LAPDGWKWTSPVGSYPANGFGLFDMAGNVWEWTTDWYTDRHAPDAAKPCCLPSDPRGG
                     TREG"
BASE COUNT         1287 a         2868 c         2674 g         1342 t
ORIGIN      
        1 acgcccggcc gcccgaggcg gtcgacgagc gtcccgagcg ccggcggcgc gggggcgaca
       61 agcccttcgt gctcttcgtc tcctacccga gcccgcaccc gcccttctcg gtgccggccg
      121 agttctggga aggcatcgac gaggcgggcg tgcggctgcc gaccggctgg cgcccggaag
      181 agcggagcga gcaccccgcc gaccggcatc tccgacacat catggacacc ggcccgctga
      241 tcgacgaggc gcagatgcgg cgcatccagg cgggctacct ggcgctgatc gcccatctcg
      301 acaccgagat cggcaagctg ctcgagggcc tcgaggaagc gggcctcgcg caggacacgc
      361 gcgtcctcta cacgagcgat cacggcgatc tcgccggcca gaacgggctc ctcggcaaga
      421 gctgcctcta cgagggctcg atcggcgtgc cgctggtcat ggccgggccg ggcattcccg
      481 agggccggcg agtggcgcag atcgcgggcc atgtcgatct ctatccgacc atcgccgagg
      541 ccgtcggcgg ccggctcgag gccgcggacg acgacctgcc cggccgctcg ctctttccgg
      601 ccctgcaggg tgaggaggca gccgtccagg gctttgccga gtatcacgcg gccggctcga
      661 aggcgggcag cttcatgctg cgcgatggcg acctcaagct gatctaccat gtcggcatgc
      721 cctcgcagct cttcgagctc gaggcggatc ccgacgagct ctcggatctc gggccggacc
      781 atccggaggt ggcacggctc gaagcactcc tgcgccggat ctgcgatccc gaggcggtcg
      841 atgcgcgggc caaggccgat cagcgggcga aagtggaatt ctggggcggt cgggaggccg
      901 tggagcagga aggcctcctc gtctacaccc cgccgcccgg tgggaaagcg gagatcgtgg
      961 catgacgaag cggcccaata tcctttggta ctgcaccgac cagcagcgct gggacacgat
     1021 ccgccggctg ggcaacccgc acatcaacac gccgcggctc gatgccttct gcgagagtgg
     1081 cgtggcgttc gacaacgcct attgccagag cccgatctgc acgccgtccc gggcgtccat
     1141 gatgaccggc cgctatcccg cctcgaccca tgttcatcgc aacggcaatg cccatttccc
     1201 ggcgggcgag acggtggtga cgaagctcct ggccgatgcc ggctacgatt gcgggctgat
     1261 cggcaagttc cacctgacgg ccgccaaggg gcacgagaag cggttcgacg acggctaccg
     1321 gctctgggag tggagtcatc atcccaagcc cgacctcgac gtcgagcacc atgcctatca
     1381 tcgctggctg gtcgagaagg gtgtcgatcc gcacgccctg cacgccagca tcgagccctt
     1441 ctgcggcgcc ggcatgccgg aagagctgca ccagacgacc tggatcaccg agcgggcgat
     1501 cagcttcatc aacgaggagc gcgacggccc ctggatgctg tcgctcaatc ccttcgatcc
     1561 gcacccgccc ttcgatccgc ccaaggcgta tctcgaccgc tacgatcccg agaagctgcc
     1621 gcccgcggcc ttccgaccgc acgacatcga gcgccagaag gccttcgccg gcatcacgca
     1681 gcagaccccc gaggccatcg atccgaccgg cccgatgccg aaggtggagc tgacggcaga
     1741 ggagcggctg accaccggct acaagccgcc gaagcagtac aacgaccgga tcgtcaaggc
     1801 cgcctactac gccatgatcg agctgatcga cacccagttc ggccggctga tcgacgcgct
     1861 ggaagcgagc gggcagctcg acgacaccat catcgtcttc cactccgacc acggcgagac
     1921 gctcggcgac cacggcctgc tctacaaggg ctgccggttc ttcgagggcc tcgtgcacgt
     1981 gccgatgatc tggtcctggc gcggcaagac caggaccgac ctgcggaccg acgccctggt
     2041 ggagctggtc gacatcgctc ccacgctcct ggaggcggcc gggctgcctg tgccggccaa
     2101 catgcagggt cgctcgctct ggccgatcct gaccggcgag gcggcgccgc agcaccacaa
     2161 ggaccacgtc atctcggagt tcaacgacgc cctgggctcg gccacgggcg agggaccgag
     2221 ccacggcagc atgtatttcg acgggcgcta caagcacatc ctctaccagg gcacgggcct
     2281 cggcgagctc tacgatctcc aggccgatcc ggacgagttc gacaaccggt tcaacgatcc
     2341 ggcgctggcc gaattgcggc tcgagctgat gcacaagcac tgcgacgcgc tgctcgcctc
     2401 gtcggatgcg ggtgtggaaa gggtgtcgat ctattgagca gccaggcata ccccaagcgc
     2461 cggtcgcggc ttgcgcagcg cgctgcaaag ctggcataga ggccggaata cgtatgataa
     2521 ccggcaagcg gctctcgggg ggcgaaaatt gatgggcaag cggcccaaca tcctgctgat
     2581 cacgtcggat cagcagcgtg gcgactgtct cggctgcgag gggcgcggcg tgaagacccc
     2641 ccatattgat ctgctcgccg agcaggggac gcgcttcgcc acctgcatca ccccgaacaa
     2701 cgtctgccag ccgacccgcg cctcgatcct gaccgggctc ctgccgcgca cccacggcgt
     2761 cgccgacaac gggctcgacc tgccgccggc gaccggtgag cagggcttcg ccggcaggct
     2821 ctccagggcg ggctaccgca gtgccttgat cggcaagccg catttcgcca cctctcacac
     2881 cttcgagccg accggcacac ctgagtgtcg gggctcgatg catctctggg gcgaggactg
     2941 gaacgggccc tatatgggct tcgaccatgt cgagctggtg atcgagggcc acaaccagtg
     3001 gccgcccgag cggccgccct cgggccagca ttacgagcgc tggtactacc aggacggcct
     3061 cggcgacctg aagaatcggc tcttcagcac caatgtcgga cccgacacgc acggggcacc
     3121 gcagacctgg cattcgggcc tgccggtcgc ctggcacaat tcgagctggg tcggcgaccg
     3181 cacggtggcc tatgtcaacg accatcaggc gaaccacagc gaccagccct tcgtgctctg
     3241 ggcgtcgttc cccgacccgc atcacccgtt cgactgcccg gtcccctgga gcctgctgca
     3301 cgatcctgcc gaggtggacc tgccggagca ccgcacgctc gatctcgacc ggcggccctg
     3361 gtggcaccgc gccgcgctcg agggcaagcc gcagatccgt gaggatctgg cgaagatccg
     3421 cgagcagtac tcgcgcgtgc ggcagctcag cgatgccgag ctgcgcgaga tcatcgccaa
     3481 cacctacggc atgctggcgc tgatcgacca caatgtcggc cggatcatgg ccgagctgca
     3541 tcgcctcggc atcgccgacg acacgctcgt cgtctacgcc agcgatcatg gcgactggct
     3601 tggtgaccac gggctcctgc tcaaggggcc gatggcctat gaggggctga tgcgcgtgcc
     3661 gctggtgatg cgcgggccgg gcgtgcccgt gggcaaggtg atcgaggacc cggtctcgac
     3721 cctggatctc ggtgcgacct tcctcgacac tgccggcgtc gagcggccgg cccattggca
     3781 cagccggagc ctcctgccgg tgctgcgtgg cgacgagcat cgcgagttcg cgctcaacga
     3841 gtgggagctc aacgcgtcgc gctgcggtgt cgagctcaag ctcaggacgg tgcgcacgcg
     3901 tacccaccgg ctgacgctgg agctggtttc gggggccggc gagctctacg atctcgtggc
     3961 cgacccgcac gagatgaaca atctcttcga cgatccggca tgcgcggccg tccagggcgc
     4021 cttgagcgcg atgatcgaca gccgacccga cgacgcgttg gagccacccc tgacgccggt
     4081 cggcatggct tgaaagtggt gggatcaggg gattcgcgtc gcggatcgac aacgaagcag
     4141 ccagagcaca ggaggtgcag gaccatgtca ttcacccgcc gtcataccct cgggatcggg
     4201 gccggcctcg ccggcagcgc ttttctgccc aggctggcca tggcgcagga gtggccgacc
     4261 cggccgatca ccatcgccat ggctgcaccg gcgggcgggg ccaccgatct cggcacgcga
     4321 gccgtcaccg agctgatgcg ccagcctctg ggagcgcgcg ccatcgcctt ccaggacatg
     4381 tccggtgccg gcggcgtcca ggccatggac tacatcctgc aacaaccggc gacagggcat
     4441 ttctggctgg gcgccaatga cgtgatcgag gcctatccga gcctcggccg gatcgactac
     4501 gactggcggg atttcgactt ctggatgtcg ggcggcacga gctgcgccat cgtcgtgccg
     4561 ggcaacagcc cctatgaatc cttcgaccag tgggtcaacg cggtaatgga gaagccgggc
     4621 gaggtggcca tcgcctcgac gccttcgggc tcgctctggt cgaacgccgc ggtctacctg
     4681 caaaagcaga tgggcctcga tttccgcctc gccaattatg ccggcggcgg cccgaccgtg
     4741 cgcgcggtcc tggccggcga gacgagctgc gcttcggtcg gcgtcacccc gacggtcaac
     4801 ttcctcaagt ccggcgagct gcgcgccctc gccgcctgga cgcccgaggc ctgggaggtg
     4861 gcgggcacca ccattccctc gatcctcgac tatgtcgacg acccgatcct cgaggccacg
     4921 ctgccctgga ccaacatcca cggcatcgcg gtcaggaagg gtgtcgacga ggcgatcctc
     4981 gagaagatcg acgccgcctt cgctaccgcg gtcaacgatc cctcgatggt cgaggtctat
     5041 gacagcaagg ccttctttcc gttccgcgcc ccgcgccagg tggccaacga catgatgggc
     5101 aagcgcacgg cgctgcaggc ctacatcatc gaggtcattc tcggcaccgc ggtgaagacc
     5161 cgcgacgagc tcggcatcgc caagatcgag gactcctgag ccagtcgggc ggtgccctcc
     5221 ggcgccgccc gcaagctcca tccggatgag cagcatggac cagagcggga ctgggccggc
     5281 ccggccggag ccagccagga gaaaggcctt ctacgccagc ccggagaagg cggcgccgga
     5341 cttcgtcacc ggcgtcgtgc tctttctcct gagtctctac gtcctggtca cgtcctaccg
     5401 gatgccgcat ttcgccggta gcggctggct cggctcgccc gggctgaccc cggggctgat
     5461 cgctctcgtt ctcctgctcc tgtcgctggc cttgatgttc cgcgcccgcg acttccgtct
     5521 caggctcggt ccgctgcagc cgcggatcga agggctcagg gccggtgcct gcttcgccat
     5581 gatcttcgtc tacatcctgg tcacaccgct gatcgggtac gtgccggcga ccttcgcgct
     5641 ccttttcgtc ttccagaccg cctatgccag gaagctctcg gtccgctacg tgctcatctg
     5701 gagcatcggc ctgagcgcgc tcttgacgtt cattctctgg tacgtgttcg gcgagatctt
     5761 cttcgtgccg ctgccgtgac caagatcacc ccatcgttcg gtcgagccag gaggtagcgg
     5821 gatgctcgag gaagtcggcc ggctgctgct cgatccctgg gcgatcttca cgatcttctg
     5881 gtcgatgacg ctcggcatca tcatgggggc catgcctggc ctcaccggcc ccatggccat
     5941 ggcgctcctg ctcggcatcg cgtacacgat gcagaccgag tactcgatcc tcgccatgat
     6001 gctgatctac atgggcgggg tctatggcgg cagcctttcg gccatcctcc tcaacatccc
     6061 gggcgccccg gcctccgccg cgaccgcgat cgacggctac cagctcgcct tgcagggcaa
     6121 ggccggcaga gccatcgcgc tgaccaccat cgcgtcgtgc atcggcacgc tgatcgccat
     6181 cgccttcctc gccttcctca ccccatggct ggtcaaggtc tccctgctgt tcacctcctg
     6241 ggagctgctg ctgctcgtgc tgctcggcgt gatgatggcg ggatcgctca ccggcgacga
     6301 tccgatcaag ggctggatcg ccggcatggt cggcctcctg atcgcgcagg tcggcctcga
     6361 cgacctgcac atgcacccgc gcttctcctt cggctcgcac tatctcgagg ccggggtcgg
     6421 gctggtggcg gccatcgtcg ggctcttcgg catcagccag gtggtcgtgg cgatgcgcga
     6481 tcgcgacatc cacctcgagc cggtcaccca caagatcggc cgcatcatgc cgaccctcga
     6541 ggacctcaag ggccgggtcc gggtggtgct gcaatcgggc gtgatcggca gcatcgtcgg
     6601 catcctgccc gggctcggcg ccgacatggg ggcttggatc tcctatgtcc tggccaagcg
     6661 caccagccgc aagccggaac tctacggcaa ggggtcgacc gagggtgtga tcgccgccga
     6721 gaccggcaac aacgccgtcg tcccctcgtc actgatcccg gtgatcgcct tgggcctgcc
     6781 gggcagcggc ggcgcggcga tcatcatggc cgccctcttc gtgcacggcc tgacacccgg
     6841 accgatgttc ctcttcgaga atctcgacct cttcgagtac atggtgatcg ggctgctctt
     6901 cggcgtcgtc gccatgctga tcgtcggcct ggccctcgcc cacgtcatca tccacgtgct
     6961 gctgatccct cgggagaaca tcatggccgt ggtgatcgcc atgggcgtga tgggcgccta
     7021 cgcctcgaag ctgcaatacg gtgacgtctg gatgatgctg gccttcggcg tgctggccta
     7081 tgtgatgcgt cggctcgatt tcccgctggc gcccatggtg ctcggcatcg tcctcggcag
     7141 cctgctcgac gagttcctgc gcaatgccat gatcatcggc gacggcgatc tctcgccgct
     7201 cgtgacccga ccgatcgccc tcgggctcct catcgtgctg gtcctgacgc tggtcggcag
     7261 cctgccggtg ctccgccgtt cggtcagccg gctcatcgcc ggcgcccgcc ggccggcggc
     7321 acaggccgaa tgaccagcct cgcccgccgg ctgcgacgca agggcacgcg ccggccggct
     7381 gcacgcgccg tgccagcggt ggtggtgccg gcgggccagc ccgatctcga cacgatggtg
     7441 cggctcgaag gtggcgagtt ccgcatgggc tcggatcgcc attatcccga ggaggcgccg
     7501 tcccggcggg tccgcgtcga cgccttctgg atcgatccgt accccgtcac caatgccgac
     7561 ttcgcccgct tcgtcgctgc caccggctat gtcaccgagg ccgagcggac gatcgatcct
     7621 gctctctacc ccggcatcga tcccggcctg ctgaagcccg gtgccctggt cttccgtccg
     7681 acccagggcc cggtagacct caaggatttt cgccagtggt gggagtatgt gcccggtgcc
     7741 tgttggcgcc ggccgggggg ggcgggcgcc gatgccctgg ccgaccatcc cgtggtccac
     7801 gtcaccatcg gggatgcgct ggcctatgcc cgctggtgcg gcaagacact gcccaccgag
     7861 gcgatgtggg agttcgcggc ccgtggcggg ctcgaggacg ccgactattg ctggggcaac
     7921 gagttcgagc ccgaggggcg atccatggcc aagacctggc atggccgctt tccctacgag
     7981 aacctcgctc cagacggctg gaagtggacc tcgccggtcg gcagctatcc ggccaacggc
     8041 ttcgggctct tcgacatggc tggcaatgtc tgggagtgga ccaccgactg gtacaccgac
     8101 cggcacgcgc ccgacgccgc caagccctgc tgcttgccga gcgatccgcg tggcggcacg
     8161 agggaaggca g
//
LOCUS       DAOTFI010000221         8003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10858_length_8003_cov_6.813286, whole genome shotgun sequence.
ACCESSION   DAOTFI010000221 DAOTFI010000000
VERSION     DAOTFI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_8003_cov_6.813286"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="P5558_18920"
     CDS             <1..573
                     /locus_tag="P5558_18920"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRY26448.1"
                     /translation="WKRETLGEPWQKGETLVIGIGQGYMLATPLQLAIMTARLCNGGR
                     AVTPWFYRDPAAEPPAVGSVGVSQASLDHVLKGMSMVVNSQRGTAYRQRLTLEGVQMA
                     GKSGTSQVRRITRAERAANLHKRKDRPWEHRHHALFVAFAPTDHPRYAVSVIVEHGQS
                     GSGAAAPIVRDIMTRVLEIGPGVATVAASL"
     gene            606..1751
                     /gene="rodA"
                     /locus_tag="P5558_18925"
     CDS             606..1751
                     /gene="rodA"
                     /locus_tag="P5558_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRY26449.1"
                     /translation="MASRVRIRPQELRFAEKLASLHWPFVALLLLIGCVGYLVLYSAG
                     GGNHQPWAWRHGARLAFGLVVMLTVALIDIRFWYRWAYVLYGGAFAMLVGVEILGEIN
                     KGAQRWVDLGFVQIQPSELMKIGLVLCLARYFHSAYLDEVKRPLYLLPPLAMIGLPAL
                     LVLKQPDLGTSIMLLAVGGSLLFLAGVRLWKFGLAIAAVAAALPVAWSGLHDYQRQRV
                     LTFLDPETDPLGAGYHIIQSKIALGSGGFWGKGYLMGSQAQLSFLPEKQTDFAFTMLA
                     EETGFVGALIVLLLYLAVVGLSLAVALRATHQFGRLLAMGIGINFFLYVLINVAMVTG
                     LIPVVGVPLPLISYGGTAMLTTLVGFGFLLSVDVHHTTNIPRFPTDL"
     gene            complement(1788..1862)
                     /locus_tag="P5558_18930"
     tRNA            complement(1788..1862)
                     /locus_tag="P5558_18930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1828..1830),aa:Val,seq:tac)
     gene            complement(1951..5460)
                     /gene="dnaE"
                     /locus_tag="P5558_18935"
     CDS             complement(1951..5460)
                     /gene="dnaE"
                     /locus_tag="P5558_18935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRY26450.1"
                     /translation="MPHAPFVHLRVRTSYSLLEGAIDHKALAARAAALGMPAVAVTDN
                     ANLYGVMSFCSATLAAGVQPIIGALLPLALAEGQRSFRRERQHSPALLPVLAQNAAGY
                     GSLMKLMSTLHFEAGSDKGLTPDQLARRSEGLIVLTGGADGPLGRLLRRDDKDAALVL
                     LDHLAEAFAGRLYVELQRHGEEAEAMIEPTLLDLAFDRDLPVVATNDCLFLDPAMHAA
                     HDALICIAEGQHVGNEDRRRFSLDYRFKTAAEMAALFADLPEAIENTLLIARRCAALA
                     PAREPILPPFAGADGQGEVEELRVQARTGLARRLERLGVEGEARAPYQERLEYELDVI
                     IQMQFPGYFLIVSDFIRWAKQQGIPVGPGRGSGAGSVVAWSLEITDLDPLRFGLLFER
                     FLNPERVSMPDFDIDFCQDRRDEVIRYVEGRYGADRVAHIITFGKLQARAALRDVGRV
                     LGLPFGLVDKISKLVPFNPANPPTLAQAVEMEPRLAEARDGDPAVARMIEIALELEGL
                     PRHASTHAAGVVIGDRPLDELVPLTRDPRSGSAVTQFNMKDVETAGLVKFDFLGLTTL
                     TMLRMAEDLVNRRGTALDLAALPLDDAATYALLSRAETAGVFQLESGGMRDALRKLKP
                     DCFEDIIAMVSLYRPGPMDNIPRYINVKHGAETAEYLHPMLEPILKETNGVIIYQEQV
                     MEIAKQLAGYTLGGADLLRRAMGKKIKAEMDAQRATFVDGAMARGISRRMADVIFDSV
                     AKFASYGFNKSHAAAYALLAYHTAYLKANHPLEFYAAVMTMEMANQEKLAAYRHEMRQ
                     RGIELLPPHVNASLPVFSVEDGSPPNGSAGSRTGGGVRFALAAIKGVGQAAMQALCAE
                     RAAGGAFTGLCDLTARLAGKGFNRRLLEALARAGALDGLAANRATLMAGLEQAIAHAG
                     AVAAARESTQEGLFGSNGDDVPEPRIATLEEWPLGERLGHEFDVLGVYLSAHPLDGYA
                     AALAERGVVAAAQVLEAPGRFDGATAELAGVIVARKEKRTERSRFAFLQVSDISAQFE
                     VMLFADVLERSRDLLEIGTAVLITADIRADGDEIKFAAQRLTRLDDVAAGRGELVEIE
                     LSEAGAAGRLRPHLREGEKGAQVRLVLGLPNGREVVVALPAAISLSHARRPDVERIAG
                     VTRIAACGSAANAHEPAARGTFH"
     gene            complement(5539..6273)
                     /locus_tag="P5558_18940"
     CDS             complement(5539..6273)
                     /locus_tag="P5558_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26451.1"
                     /translation="MSEPVLVLSGIERRFRQGATTLPVLRGADLTLAPGELVALVGPS
                     GSGKSTLLHIAALLERPSAGEVTIDGRACGRLGDRERTGLRRNRIGFVYQFHHLLADF
                     TALENAMLPQLLAGRSRAQAKARAADLLGRLGLGERLGHRPGRLSGGEQQRVAIARAL
                     ANRPGLLLADEPTGNLDPATSARVLDLLVEVVRETGTAGFIATHNPDIAAAMDRTLEL
                     VDGRLRPWTAPPATGRGEPVRQTTPA"
     gene            complement(6266..7507)
                     /locus_tag="P5558_18945"
     CDS             complement(6266..7507)
                     /locus_tag="P5558_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRY26452.1"
                     /translation="MRSAERLIAWRYLRPTRGEGFISVIAAFALVGIALGVGTLIVVL
                     AVMNGFRSELLGRVLGVNGHATVVAGPEGLADSDVLVTRLRTVDGVLDAMAYVEGQVM
                     ASGNNVASGAIVRGVRAEDLARRAVFADNIVEGSLARLDEGGTILIGSRMGQRMGLGV
                     GDRVSLISPQGAATAFGTVPRIQSFEIAGIFEVGMFEYDNSFVYIGIDDAMRYFQLDD
                     RVTAVEILVDDPEMVRQLRPTLTATVAGQGRLVDWQQLNSHFFNALEVERNVMFLILT
                     LIILVAAFNIITGITMLVKSKGRDIAILRSMGATQGAVMRIFFLAGASIGVAGTLIGF
                     GLGIAFALNIDSIRLFLEGLTGAELWSAEIRFLSRLPAEINPDDVTRVVLMGLGLSFL
                     ATLYPAWRAARVDPVEALRYE"
     gene            complement(7507..>8003)
                     /locus_tag="P5558_18950"
     CDS             complement(7507..>8003)
                     /locus_tag="P5558_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRY26453.1"
                     /translation="RSGRGIEVGHIFYFGTKYSEPMGATVIGPDGATQPVHMGSYGIG
                     VSRLVGALIEANHDDKGIIWPEAVAPFKVGIVDLRPDDALSHGLAEELHERLQRAGVD
                     VLLDERDERAGAKFATMELIGLPWTVTVGPRGAKEGKVELKARASGETVELDPDALVQ
                     RLTA"
BASE COUNT         1205 a         2738 c         2828 g         1232 t
ORIGIN      
        1 tggaagcgcg agaccctggg cgagccctgg cagaagggcg agacgctggt gatcggcatc
       61 ggccagggct acatgctggc cacgcccttg cagctcgcca tcatgaccgc ccgcctgtgc
      121 aatggcgggc gagccgtcac gccctggttc taccgcgacc ccgccgccga gccaccggcg
      181 gtcggctcgg tcggcgtgtc ccaggcttcc ctcgatcatg tgctcaaggg catgtccatg
      241 gtggtcaaca gccagcgtgg cacggcctat cggcaacgcc tgacactcga gggtgtgcag
      301 atggcgggca agtccggcac ctcgcaggtg cggcggatca cccgggcgga gcgcgcggcc
      361 aatctgcaca agcgcaagga ccggccctgg gagcatcgcc accacgctct cttcgtcgcc
      421 ttcgcgccga ccgaccaccc gcgctatgcg gtctcggtga tcgtcgagca cggccagagc
      481 ggcagtggtg cggcggcccc gatcgtccgg gacatcatga ccagggtgct cgagatcggc
      541 cccggggtcg ccaccgtggc ggccagcctg tgagtggccc aatgaaggtc ggattcgact
      601 gagccatggc gagccgggtc cgcatccgcc cgcaggagct gcgcttcgcc gagaagctgg
      661 cgtcgctgca ctggccgttc gtcgccctgc tcctgctgat cggctgcgtc ggctatctcg
      721 tcctctattc ggccggcggc ggcaaccacc agccctgggc ctggcgccac ggtgcgcggc
      781 tcgccttcgg cctcgtcgtg atgctgaccg tggcgctgat cgacatccgc ttctggtacc
      841 gctgggccta tgtgctttat ggcggtgcct tcgccatgct ggtcggtgtc gagatcctgg
      901 gcgagatcaa caagggcgcg cagcgttggg tcgacctcgg cttcgtccag atccagccct
      961 cggagctgat gaagatcggt ctcgtgctct gcctcgcccg ctatttccac agcgcctatc
     1021 tcgacgaggt caagcggccg ctctatctgc tgcccccctt ggcgatgatc ggcctgccgg
     1081 cgctcctcgt cctcaagcag ccggacctcg gcacgagcat catgctgctc gccgtcggcg
     1141 gctccctgct ctttctcgct ggcgttcgcc tgtggaagtt cggcctcgcc atagccgctg
     1201 tcgccgccgc cctgccggtc gcctggagcg gcctgcacga ttaccagcgc cagcgcgtgc
     1261 tgacctttct cgatcccgag accgacccgc tcggcgccgg ctaccacatc atccagagca
     1321 agatagcgct cggctcgggc gggttctggg gcaagggcta tctgatgggc agccaggcgc
     1381 agctcagctt cctgccggag aagcagaccg atttcgcctt caccatgctc gccgaggaga
     1441 ccggcttcgt cggcgcgctc atcgtgctgc tgctctatct ggcggtcgtc ggcctgtcgc
     1501 tggccgtcgc ccttcgggca acccaccagt tcgggcggtt gctcgccatg ggcatcggca
     1561 tcaatttctt tctctatgtg ctcatcaacg tcgccatggt gacgggcctg atcccggtgg
     1621 tcggcgtgcc tttgccgctg atctcctatg gcggcacggc gatgctcacc acgctcgtcg
     1681 gcttcggctt cctgctgtcg gtcgacgtgc accacaccac caacatcccg cgctttccga
     1741 ccgatctctg actgtgctct cggccgtcac gggggagaag tggagcctgg tgcgggcgga
     1801 aggggtcgaa cctccgacac cggcggtgta aacgccgtgc tctaccgctg agctacgccc
     1861 gcacgggcgc gaccatagaa acggtcatgg ccgcgggcaa ggggtggcgg gcaaggcacg
     1921 acgaccgtac ctgccctcgg cagcacaagc tcaatggaag gtgccgcgcg ccgccggctc
     1981 gtgcgcattg gcggcgctgc cgcatgcggc gatccgcgtg acccccgcga tccgctccac
     2041 atcgggccgg cgtgcatggc tgagcgaaat ggcggcgggg agcgccacca cgacctcgcg
     2101 gccattcggc aggccgagga cgagccgcac ctgggccccc ttttcgccct cgcggagatg
     2161 aggcctcagc cgcccggcgg cgcccgcctc gctgagctcg atctcgacca gctcgcccct
     2221 gcccgcggcc acgtcgtcga gtcgtgtcag gcgctgcgcc gcgaacttga tctcgtcgcc
     2281 atcggcacgg atgtcagcgg tgatcagaac ggcggtgccg atctccagaa ggtcgcggga
     2341 gcgttccagc acgtcagcga acagcatgac ctcgaactgc gccgaaatgt cggaaacctg
     2401 caggaaggcg aagcggctgc gctcggtgcg cttctccttc cgggcgacga tcacgccggc
     2461 gagctcggcc gtggcaccgt cgaagcggcc gggcgcctcc agcacctggg cggcggcaac
     2521 gacgccgcgc tcggcgagcg ccgcggcgta gccatcgagc ggatgcgccg agagatagac
     2581 tccgagcaca tcgaactcgt gaccgagacg ctcacccagg ggccactcct cgagggtcgc
     2641 gatgcggggc tcgggcacat cgtcgccatt gctgccgaag agcccctcct gggtgctctc
     2701 ccgggccgcc gccaccgccc cggcatgggc gatcgcctgc tcgagcccgg ccatcaacgt
     2761 cgcccggttg gccgccaacc cgtccagcgc gccggcccgc gcgagcgcct cgagcaggcg
     2821 acggttgaag cccttgccgg cgagcctggc ggtcagatcg cagagacccg tgaaagcgcc
     2881 tccagccgcg cgctcggcgc agagcgcctg catcgcggcc tggcccaccc ccttgatcgc
     2941 ggcgagcgcg aagcgtacgc cgccccccgt ccggctcccc gccgagccgt tcgggggcga
     3001 cccgtcctcg accgagaaaa ccggcaggga agcgttgacg tgcggcggca ggagctcgat
     3061 gccgcgctgg cgcatctcgt gccggtaggc ggcgagcttc tcctggttcg ccatctccat
     3121 ggtcatcacc gccgcgtaga actcgagcgg gtggttcgcc ttgagatagg ccgtatggta
     3181 ggcgagcagc gcataggcgg cggcatggct cttgttgaag ccgtaggagg cgaacttcgc
     3241 caccgaatcg aagatcacgt cggccatcct gcggctgatg ccgcgcgcca ttgccccgtc
     3301 gacgaaggtg gcgcgctgcg cgtccatctc ggccttgatc ttcttgccca tggcccggcg
     3361 caggagatcg gcgccgccga gcgtgtagcc ggcgagctgc ttggcgatct ccatcacctg
     3421 ctcctggtag atgatgacgc cgttcgtctc cttcaggatc ggctcgagca tggggtgcag
     3481 gtattccgcc gtctcggcgc cgtgcttgac gttgatgtag cgcgggatgt tgtccatcgg
     3541 gcccggccgg tagagcgaga ccatggcgat gatgtcctcg aagcagtcgg gcttgagctt
     3601 ccggagcgca tcccgcatgc cgccggattc cagctggaac acacccgccg tctccgcccg
     3661 gctgagcagc gcgtaggtgg ccgcgtcgtc gagcggcagg gccgccagat cgagcgccgt
     3721 gccgcgccgg ttcacgagat cctcggccat gcgcagcatg gtcagcgtgg tgaggccgag
     3781 gaagtcgaac ttgacgaggc cggccgtctc gacgtccttc atgttgaact gggtgacggc
     3841 gctgccgctc ctgggatcgc gggtgagcgg cacgagctcg tcgagcggcc ggtcgccgat
     3901 caccacgccc gccgcgtggg tcgaggcgtg gcgcggcagg ccctcgagct cgagggcgat
     3961 ctcgatcatc cgcgccacgg ccggatcgcc gtcgcgcgcc tcggcgagcc gcggctccat
     4021 ctcgaccgcc tgcgccagcg tcggcgggtt ggccgggttg aaaggcacga gcttcgagat
     4081 cttgtcgacg aggccgaaag ggagcccgag cacgcggccg acatcgcgca gcgccgccct
     4141 cgcctgcagc ttcccgaagg tgatgatgtg ggcgacccgg tcggcaccgt agcgcccctc
     4201 gacatagcgg atcacctcgt cacggcggtc ctggcagaaa tcgatgtcga agtccggcat
     4261 cgagacgcgc tcggggttga ggaagcgttc gaagaggagg ccgaagcgga gcgggtcgag
     4321 gtcggtgatc tcgagcgacc aggcgacgac cgagcccgcc cccgagccgc ggcccgggcc
     4381 caccgggatg ccctgctgct tggcccagcg gatgaaatcc gagacgatga ggaagtagcc
     4441 cggaaactgc atctggatga tgacgtcgag ctcgtattcg agccgttcct gatagggtgc
     4501 cctggcctcg ccctcgacgc ccagccgctc gagccgccgg gcgagaccgg tacgggcctg
     4561 gacccgcagc tcctcgacct cgccctggcc atcggcgccg gcgaaaggtg gcaggatcgg
     4621 ctcgcgtgcc ggtgccaggg cggcgcaacg gcgagcgatg agcagcgtgt tctcgatcgc
     4681 ctccggcagg tcggcgaaga gcgctgccat ctcggccgcc gtcttgaagc ggtagtcgag
     4741 cgagaaccgg cggcggtcct cgttgcccac atgctgcccc tcggcgatgc agatcagcgc
     4801 atcgtgcgcg gcgtgcatgg ccggatccag gaagaggcag tcattggtgg cgacgacggg
     4861 caggtcgcgg tcgaaggcga gatcgagcag cgtcggctcg atcatcgcct cggcctcctc
     4921 gccgtggcgc tgcagctcga catagagacg ccctgcgaac gcttcggcca gatggtcgag
     4981 caggaccagg gcggcatcct tgtcgtcgcg ccgcagcagc cggccgagcg gcccgtcggc
     5041 gccgcccgtc aggacgatca ggccttcgct ccgccgtgcc agctggtccg gggtgaggcc
     5101 cttgtcgctc ccggcctcga aatggagcgt gctcatcagc ttcatcaggc tgccgtagcc
     5161 ggcagcgttc tgggcgagca cgggcagcag ggcgggactg tgctgtcgct ctcggcggaa
     5221 cgagcgctga ccctcggcca gcgccagcgg caggagggca ccgatgatcg gctggacgcc
     5281 ggcggcgagg gtcgcggagc agaacgacat cacgccgtag agattggcat tgtcggtgac
     5341 ggcgaccgcc ggcatgccga gtgccgcggc ccgcgcggcc agcgccttgt ggtcgatcgc
     5401 cccctcgagc agcgagtagg aggtgcggac ccgcaggtgg acgaaggggg catgcggcat
     5461 cacgggccct agatctggtg gtgaggcccc tgtcctaccg caggatgctc gaaaagtgaa
     5521 ccaattgcgg cgggcgggtc aggccggtgt cgtctgccgg acgggctctc cgcggccggt
     5581 cgccggcggc gccgtccagg ggcgaaggcg gccgtcgacc agctcgagcg tgcggtccat
     5641 ggcggcggcg atgtccgggt tgtgcgtggc gatgaagcca gcggtcccgg tctcgcgcac
     5701 cacctcgacc aggaggtcga gcacgcgagc cgaggttgcc ggatcgagat tgccggtcgg
     5761 ctcgtcggcg agcagcaggc ccggccggtt ggccagggcg cgggcgatgg cgacccgctg
     5821 ctgctcgccg ccggagagcc ggcccgggcg atgcccgagg cgctcgccga gcccgaggcg
     5881 accgagcagg tcggccgccc gcgcctttgc ctgcgcccgg ctgcggcccg ccaggagctg
     5941 tggcagcatg gcgttctcga gggcggtgaa atcggcgagg aggtggtgga actgatagac
     6001 gaagccgatg cggttgcggc gcaggccggt gcgctcgcga tcgccgagcc ggccgcaggc
     6061 ccggccgtcg atggtgacct cgccggcgct cggccgctcg agcagggcgg cgatgtggag
     6121 cagcgtcgac ttgccggagc cggatggccc gaccagggcg acgagctcgc cgggtgccag
     6181 cgtgagatcg gcgcccctga gcaccggcag ggtggtcgct ccctggcgaa agcgccgttc
     6241 gatgccgctc aggaccagca ccggctcact catagcgcag cgcttccacc ggatcgacgc
     6301 gcgccgcccg ccaggccggg tagagcgtgg cgaggaagga gaggccgaga cccatcagca
     6361 cgacccgggt gacgtcgtcg ggattgatct cggccgggag gcgggaaagg aagcgaattt
     6421 ccgccgacca gagctccgcc ccggtcaggc cctccaggaa aaggcggatg ctgtcgatgt
     6481 tgagggcgaa ggctatgccg aggccgaagc cgatcagggt gccggcgacg ccgatgctgg
     6541 cgccggcaag gaagaagatg cgcatcacgg caccctgcgt ggcgcccatg ctgcgcagga
     6601 tggcgatgtc gcggcctttg gacttcacca gcatggtgat gccggtgatg atgttgaagg
     6661 cggcgacgag gatgatgagg gtgaggatca ggaacataac gttgcgctcg acctcgagcg
     6721 cattgaagaa atgcgagttg agctgctgcc agtcgaccag gcggccctgc ccggcgaccg
     6781 tcgcggtgag ggtcgggcgg agctgccgca ccatttccgg gtcgtcgaca aggatctcca
     6841 cggcggtcac gcggtcgtcg agctggaagt agcgcatcgc gtcgtcgatg ccgatataga
     6901 cgaagctgtt gtcgtactcg aacatcccga cctcgaagat gccggcgatc tcgaagctct
     6961 ggatccgcgg cacggtgccg aaggcggtgg cggccccctg cggcgagatc aggctgacgc
     7021 ggtcgccgac gccgaggccc atgcgctggc ccatgcggct gccgatcagg atcgtgccgc
     7081 cctcgtccag ccgcgccagc gagccctcga cgatgttgtc ggcgaagacg gcgcggcggg
     7141 cgagatcctc ggcccgcaca ccgcgcacga tggcgccgga ggcgacattg ttgccgctgg
     7201 ccatcacctg accctcgaca taggccatgg cgtcgagcac gccgtcgacc gtgcgcaggc
     7261 gggtcaccag cacgtcgctg tcggcgaggc cctcgggccc ggcgaccacc gtggcatgac
     7321 cgttgacgcc gagcacgcgg ccgagcagct ccgagcggaa gccgttcatg accgcgagca
     7381 cgacgatgag cgtgccgacg ccgagtgcta tgccgaccag cgcgaaggcg gcgatcaccg
     7441 agatgaagcc ttcgccccgg gtcggtcgaa gatagcgcca ggcgatcagc cgctcggcgc
     7501 tgcgcatcag gcggtgagcc gctggacgag cgcatccggg tcgagctcga cggtctcgcc
     7561 gctggcgcgc gccttgagct cgaccttgcc ctccttggca ccgcgcggcc ccactgtcac
     7621 ggtccagggc aggccgatca gctccatcgt ggcgaacttc gccccggcgc gctcgtcgcg
     7681 ctcgtcgagg aggacatcga ccccggcccg ctgcagccgt tcatggagct cttcggcgag
     7741 gccgtgggag agcgcatcgt ccggccgcag atcgacgatg ccgaccttga acggtgccac
     7801 ggcctcgggc cagatgatgc ccttgtcgtc gtggttggcc tcgatgagcg caccgaccag
     7861 ccgcgacacg ccgatgccgt aggagcccat gtgcaccggc tgcgtggcac cgtcggggcc
     7921 gatcacggtg gcccccatgg gctcggaata cttggtgccg aaatagaaga tgtggccgac
     7981 ctcgatgccg cggccgctgc gca
//
LOCUS       DAOTFI010000222         7940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_10964_length_7940_cov_7.378186, whole genome shotgun sequence.
ACCESSION   DAOTFI010000222 DAOTFI010000000
VERSION     DAOTFI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10964_length_7940_cov_7.378186"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="P5558_18955"
     CDS             complement(<1..400)
                     /locus_tag="P5558_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26454.1"
                     /translation="MAGPLLLEMRGISKAFPGVQALDRVDFDLRAGEVHVLFGENGAG
                     KSTLINIIAGTFAPDAGSFEFNGEPMRGLTPHRARVIGISPVFQEFSLVAEMTVEENL
                     FLGREIAKAGVLNRGAMRERAARTIADLGFA"
     gene            complement(475..1548)
                     /gene="torT"
                     /locus_tag="P5558_18960"
     CDS             complement(475..1548)
                     /gene="torT"
                     /locus_tag="P5558_18960"
                     /inference="COORDINATES: protein motif:HMM:NF008185.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HRY26455.1"
                     /translation="MRMTSKHALIAATALATTIGIGGSASAEGEWWPVKVYDASSGEN
                     VEVEYSPVEKAAAPHNICVLFPHMKDSFWVAVAYGIVEEAERQNVNMTLFEAGGYENL
                     PRQISQFDDCMAGGYDAIVIGAISEAGLALKFEEAIAAGIPVISTVNPVADAETTAKM
                     FVEFDVMGEQTGSYLVEHLAGAEANVVNFPGPAGSGWAELFNDGFKRAIEGVDNVTLL
                     DEKFGDSGVSVQLQLIQDALQAYPEMNVIWGTAPTAEAGIAAVAEVGREGEIMIMSSY
                     ENQAMLDALNRGDILGFATQYPVLEGRVAIDMAVRALEGAPLMTFVKPIPDMIAAGTV
                     DQIDMSQVLAPADWTPVYSVTAE"
     gene            1738..2421
                     /locus_tag="P5558_18965"
     CDS             1738..2421
                     /locus_tag="P5558_18965"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY26456.1"
                     /translation="MAGGLRSLPRDTLRDRVYEGLRNSLLRGSFEPGEPLTVRSLATA
                     FGTSPTPVREALQQLVAEQALTAEPNRSYRIPRVSRETFLEVRDIRVELEGLAAANAA
                     AHIRPATLRRLELAVERMGKAIRSQDLKTYLAANQAFHFIVYEASGSPILLRTISSLW
                     LLIGPSLNMLFSDLELVEGLQDQHHAVIEAFRRGDADAARRAVQADILAAGMHIGRQM
                     SEPGSSTSS"
     gene            complement(2444..3589)
                     /locus_tag="P5558_18970"
     CDS             complement(2444..3589)
                     /locus_tag="P5558_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRY26457.1"
                     /translation="MIRVATFAGPGTAPVIREVARPQVPKNAALIQIGACGVCGTDLH
                     ILKGHWPKPLPWPFTLGHEIAGVIVERGAELAEDFMGKPLEVGSRVMIPPLMPCGRCH
                     YCVHYPQNANKCLTPVYYGRYLGFDKAPHLWGGWAEHVYVDLDMLPGTKIYKLPDDMP
                     LRLGALAEPLTSCIRAFARAQRAGGFGYGDTVVIQGSGPIGILAIAAAQEMGAGRVLC
                     VGAPEEPRLRLARRFGAEATVDIEELKTPQERIARVRELVGGFGADLVMDCSGHPSAG
                     PEGIEFLRDGGTYVEMGQFTDAGSIETSWHRICAKDISVLGSWAFTGNDLPLGVDMLY
                     RARDSYPFLDMQTLYPFDEDGIARAIADAMAMRTVKSTIVPCPELLQ"
     gene            complement(3669..4658)
                     /locus_tag="P5558_18975"
     CDS             complement(3669..4658)
                     /locus_tag="P5558_18975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRY26458.1"
                     /translation="MSRTIDVLTAGLETSVQELPGRIGFWEQGFPPSGPMDIWSFRQA
                     NLLVGNAPDTAALECQFVGPELRFSAPALVAVTGADMTPMVDGVPVESWTTLALEAGQ
                     TLTLGPALKGARAYVGVSGGIDIPPVLGSRATFHMAGVGGVEGHAIKAGQQLPLGQAA
                     AGPRPGTRVAPEARPRIDGGREWTIEVVPGPNDDWLDEAGRARFLGADWKLTAKSNRT
                     GFRLQGPAWSFSPLATDKRPEHGSDPSNILDHGYPFGAVNLAGQTPIILVNDGPSTGG
                     FINPYTVITAAFWKLGQSRPGDIYRFRAVTVGEAQDLRRALDARCTTASLQPG"
     gene            complement(4675..5544)
                     /locus_tag="P5558_18980"
     CDS             complement(4675..5544)
                     /locus_tag="P5558_18980"
                     /inference="COORDINATES: protein motif:HMM:NF014712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HRY26459.1"
                     /translation="MIYDEPVFKPGGDRNLLVYLGDEMSFDLNFLVQTYAARIREAAL
                     PGLVELIPELASLLVSYDPDRLGYDDLIRELAGIRRGIGRLEGLELPSRLFNVPVLYF
                     DPWTAACVDDYRAKLAEKEPDPDYLVRLNGLADRRQLVRVHSGTEYWVAALGFWPGLG
                     SFLPLDPRCRLTAPKYNPPRTWTPKGTVGMGGSITCIYPDETPGGYQIFAISPMPIWD
                     PAQRLAAFRDSLALFRPGDRVRFVPIDREEHDYITARVEDGSYLHPEVGYQRFSIDAY
                     HAWLDTLDPTKRF"
     gene            5658..6548
                     /locus_tag="P5558_18985"
     CDS             5658..6548
                     /locus_tag="P5558_18985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26460.1"
                     /translation="MSPAVVNVTLLITASAIYGSVFSLNKLAAAAGWQPLQFAFAQSL
                     LAGLALAVVVLVRRELPRPSVRLILACLVIGSLFVGLPITLLSHVAPHLPAAVTTLVL
                     ALSPVVTLIFAMALGVERFRWGAVVGIACGLAGIAVLVMPGSSLGGGSAGSWFLLALL
                     APVMFGLGNVLSSILRPPVTASTAMAGGSLLGSALSLVPLLVATGLFGWPALGPATLP
                     PVLLASAVNAVFFVLLFEIIRRAGPTFFAQFNYLAVLAGVAWGAMLFDERPGLSFWLA
                     LALMLAGVFFATRAPEKASA"
     gene            complement(6555..>7940)
                     /locus_tag="P5558_18990"
     CDS             complement(6555..>7940)
                     /locus_tag="P5558_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HRY26461.1"
                     /translation="VAVNRDLARDLGVDPELLDSADGAAMLAGNVLPDGAEPLAMAYA
                     GHQFGQFVPQLGDGRAILLGEVNDRSGQRRDIQLKGAGRTPFSRGGDGRAALGPVLRE
                     YLMGEAMHALGIPTTRALAAVTTGEPVHRETTLPGAVLTRVALSHVRVGTFQFFAARG
                     DTEALARLVAYVIERHYPELGQSDQPALDLLAAVAERQARLIAMWLSVGFIHGVMNTD
                     NMSIAGETIDYGPCAFMDAYDPATVFSSIDHAGRYAFANQPRLAGWNLARLAEALLGL
                     IDSDVERAVTRATEAVEVFGRTFESELLQRMRRKIGLEMAEKDDLALVHALLDAMHKG
                     AADYTLTFRRLADAALDPALEDRVAELFATPELYRNWAVGWRVRLTRESTTLAERSAL
                     MRRVNPAIIPRNHRVEQALEAAVERGDLGPFRELLEALAQPYDDPGARCAAYMEPPCP
                     EERVLATFCGT"
BASE COUNT         1174 a         2726 c         2771 g         1269 t
ORIGIN      
        1 gggcgaagcc gagatcggcg atggtccggg ccgcccgctc gcgcatcgcc ccgcggttca
       61 ggacacctgc tttcgcgatc tcgcggccga ggaagaggtt ctcctcgacc gtcatctcgg
      121 cgaccagcga gaactcctgg aagaccgggc tgatgccgat gacccgtgcg cggtgcgggg
      181 tcaggccgcg catcggctcg ccattgaact cgaagctgcc ggcatcgggc gcaaaggtgc
      241 cggcaatgat attgatcagg gtcgacttgc cggcgccgtt ctcgccgaag aggacatgca
      301 cctcgccggc gcggaggtcg aaatcgacgc gatcgagcgc ctgcacgccg gggaaggcct
      361 tcgagatgcc gcgcatctcg agcagcaacg gccccgccat cgcgggcggc ggggccttgc
      421 gtgcttcggc cgaccgggcg gccacgggcg acccgctttc ggtcgccgtc cggctcactc
      481 ggcggtcacg gagtagacag gggtccagtc ggccggcgcc agcacctgac tcatgtcgat
      541 ctggtcgacc gtgccggcgg cgatcatgtc ggggatcggc ttgacgaagg tcatcaaggg
      601 cgccccctcg agcgcccgca ccgccatgtc gatggcgacc cgcccctcga gcaccgggta
      661 ctgcgtggcg aagcccagga tgtcgccgcg gttgagcgca tccagcatcg cctggttctc
      721 gtaggaggac atgatcatga tctcgccctc gcggccgacc tcggcaacgg ccgcgatgcc
      781 ggcctcggcc gtcggtgccg tgccccagat gacgttcatc tcgggatagg cctggagcgc
      841 gtcctggatg agctggagct gcacggagac accggaatcg ccgaacttct cgtcgagcaa
      901 agtgacgttg tcgacaccct cgatggcccg cttgaagccg tcgttgaaga gctccgccca
      961 accggagcca gcgggcccgg ggaagttcac cacattggcc tcggcgccgg ccagatgttc
     1021 gaccaggtag ctgccggtct gctcgcccat gacgtcgaac tcgacgaaca tcttggccgt
     1081 cgtctcggca tccgcgaccg ggttgacggt cgagatgacc ggaatgccgg cggcgatggc
     1141 ctcctcgaac ttgagcgcca gccccgcctc ggagatcgcg ccgatgacga tggcgtcgta
     1201 gccgcccgcc atgcagtcgt cgaactggga gatctggcgc ggcaggttct cgtagccgcc
     1261 cgcctcgaag agggtcatgt tgacgttctg ccgctcggcc tcctcgacga tgccgtaggc
     1321 gaccgcgacc cagaagctgt ccttcatgtg cgggaacagc acacagatat tgtggggggc
     1381 ggccgccttc tcgaccggcg agtactcgac ctcgacgttc tcaccggacg aggcgtcata
     1441 aaccttcacc ggccaccact cgccttcggc gctggccgag cccccaatgc cgatggtggt
     1501 tgccagcgcc gtggcggcga tcagggcgtg cttggacgtc attctcattg tcgcgcttcc
     1561 ttttccctgg cggactgccg agtgccaacc gtcgccgcct agtgacgcat cagggccttg
     1621 acttgtcaac ggcgtggtcg cctgttcggt atttgatata ccatttgccg tagccgtgac
     1681 ggagcggcaa tcgaaatcgg gaccggtgca cggatggcct tggaaggcga cggacgaatg
     1741 gcgggcggct tgcgcagcct gccacgcgac accctgcggg atcgcgtcta cgagggcctg
     1801 cgcaattcgc tcctgcgcgg cagcttcgag cccggcgagc cgctcaccgt gcgcagcctc
     1861 gccacagcct tcggcaccag cccgacaccg gtgcgtgagg cgctgcagca actcgtcgcc
     1921 gagcaggcgc tgaccgccga gcccaaccgc tcctatcgaa ttccccgggt ctcgcgcgag
     1981 accttcctcg aggtgcgcga catccgtgtc gagctcgagg gcctggcagc ggcgaacgcg
     2041 gcagcgcaca tccggccggc gacgctccgc cggctcgagc tcgccgtcga gcgaatgggc
     2101 aaggcaatcc gcagccagga tctgaagacc tacctcgccg ccaaccaggc cttccatttc
     2161 atcgtctacg aggcctcggg ctcgccaatc ctgctgcgca ccatcagctc gctctggctg
     2221 ctgatcggcc cgagcctgaa catgctcttc tccgacctcg agctggtcga ggggttgcag
     2281 gaccagcacc acgccgtgat cgaggccttc cgccgcggcg acgccgacgc ggcgcggcgc
     2341 gccgtccagg ccgacatcct ggccgccggc atgcatatcg gccgccagat gagcgaacca
     2401 ggatcgtcga cgtcgagctg agctgccttt cggccgggcg ccatcactgc agcagctcgg
     2461 ggcatggcac gatcgtcgac ttcaccgtcc gcatggccat ggcatcggcg atggcccggg
     2521 cgatgccgtc ctcgtcgaac gggtagagcg tctgcatgtc gaggaagggg tagctgtcgc
     2581 gagcgcggta gagcatgtcg acgccgagcg gcaggtcgtt gcccgtgaat gcccaggatc
     2641 cgaggacgct gatatccttg gcgcagatcc ggtgccagct cgtctcgatc gagccggcat
     2701 cggtgaactg gcccatctcg acataagtgc caccatcgcg caggaactcg atgccctcgg
     2761 ggccggcgct gggatggccg gagcagtcca tgacgaggtc ggccccgaac ccgccgacca
     2821 gctcgcgcac ccgcgcgatc cgctcctgcg gcgtcttgag ctcctcgatg tcgacggtcg
     2881 cctccgcgcc gaagcgccgc gcgaggcgca ggcgcggctc ctccggtgcg ccgacgcaga
     2941 gcacgcgacc cgcacccatc tcctgcgccg ccgcgatagc gagaatgccg atcggccccg
     3001 agccctggat gacgacggtg tcgccatagc cgaagccgcc ggcccgctgg gcacgggcga
     3061 aggcccgaat acaggaggtc aacggctccg ccagtgcgcc gaggcgcaag ggcatgtcgt
     3121 cgggcagctt gtagatcttg gtgccgggca gcatgtcgag gtcgacatag acatgctccg
     3181 cccagccgcc ccagagatgc ggcgccttgt cgaagcccag atagcggccg taatagaccg
     3241 gcgtcaggca cttgttcgcg ttctgggggt agtggacaca gtagtggcag cggccgcagg
     3301 gcatcaaggg cgggatcatc acccgcgagc cgacctcgag cggcttgccc atgaagtcct
     3361 cggccagctc ggcaccacgt tccacgatga cgccggcgat ctcgtggccg agcgtgaacg
     3421 gccacggcag gggcttgggc cagtggccct tgaggatgtg caggtcggtg ccgcagacgc
     3481 cgcacgcacc gatctggatg agcgccgcgt tcttcggcac ttgcggtcgc gccacctccc
     3541 ggatcacggg tgccgtgccg gggccggcga aggtcgccac gcgaatcatc tcccgcctcc
     3601 ctgctcgctg ctcttggccc gagcttgaac ccgggggcgc tgccggtcaa tcggcggcag
     3661 ggagaggctc agcccggctg caggctcgcc gtggtgcagc gggcgtcgag cgcgcgtcgg
     3721 agatcctgcg cctcgccgac ggtgaccgcg cgaaagcgat agatgtcgcc cggccgcgac
     3781 tggccgagct tccagaaggc ggcggtgatc accgtgtagg ggttgatgaa gccgcccgtg
     3841 gatggcccgt cattgacgag gatgatcggc gtctggccgg cgaggttgac ggcgccgaag
     3901 gggtagccgt ggtcgaggat gttggagggg tcgctgccat gctccggccg cttgtcggtc
     3961 gcgagcggcg agaacgacca tgcgggaccc tggaggcgaa agcccgtgcg gttggacttg
     4021 gcggtcagct tccagtcggc gccgaggaaa cgcgccctgc ccgcttcgtc gagccagtcg
     4081 tcgttggggc ccggcacgac ctcgatggtc cactcccgcc cgccgtcgat cctcggccgg
     4141 gcctcgggtg ccacgcgtgt tccgggccgg ggccctgccg cggcctggcc caacggcagt
     4201 tgctggcccg ccttgatcgc atgcccctcg acgccgccga cccccgccat gtggaaggtc
     4261 gcccggctgc ccagcacggg cgggatgtcg atgccgccgg aaacgccgac ataggcgcgg
     4321 gcacccttca gcgccgggcc gagggtgagc gtctggccgg cttcgagggc gagtgttgtc
     4381 cagctttcga ccggcacgcc gtccaccatg ggcgtcatgt cggcgccggt cacggcgacc
     4441 agcgccgggg cggagaagcg cagctcgggg ccgacgaact ggcactcgag ggcggccgtg
     4501 tcgggcgcgt tgccgaccag caggttggcc tggcggaagg accagatatc catgggcccg
     4561 gaaggcggaa agccctgctc ccagaagccg atgcggcccg gcagctcctg cacgctggtc
     4621 tcgaggccgg ccgtcaggac gtcgatcgtt cgcgacatgc ttgcccggtc cccttcagaa
     4681 gcgcttggtc ggatcgagcg tgtcgagcca ggcgtgatag gcatcgatcg agaagcgctg
     4741 gtagccgacc tccggatgca aatagctgcc gtcctcgacc ctggcagtga tgtagtcgtg
     4801 ctcctcccgg tcgatcggca cgaagcgcac gcggtcgccc ggccggaaga gagcgaggct
     4861 gtccctgaag gccgcgagcc gttgcgccgg atcccagatc ggcatcgggc tgatggcgaa
     4921 gatctggtag ccacccggcg tctcgtccgg atagatgcag gtgatcgagc cgcccatgcc
     4981 gaccgtcccc ttgggcgtcc aggtgcgcgg cgggttgtac ttgggcgccg tcagccggca
     5041 gcgtgggtcg agcggcagga acgagcccag gccgggccag aacccgagtg cggcgaccca
     5101 gtactcggtg ccggaatgga cccgcaccag ctgccgccga tcagcgaggc cgttgagccg
     5161 gacgagatag tccggatcgg gctccttctc ggcgagcttc gcccggtagt cgtcgacgca
     5221 ggccgcggtc caggggtcga aatagaggac gggcacgttg aagagccggc tcggcagctc
     5281 cagcccctcg agcctgccga tgccgcggcg gatgccggcg agctcgcgga tcaggtcgtc
     5341 gtagccgagg cggtcgggat cataggagac gagcagcgag gccagctcgg ggatcagctc
     5401 gacaaggccc gggagggccg cctcgcggat tctcgccgca taggtctgga ccaggaaatt
     5461 gaggtcgaag ctcatctcgt cgccgagata gacgagcagg ttgcggtccc cgcccggctt
     5521 gaagaccggc tcgtcgtaga tcatcggcgt tccggctccc ggtgcggctc gttctgcgat
     5581 atgccgtaga ctattgcgaa ggcgggtgca cgggcaaccg ccaaggccgc gtcggcctga
     5641 agcggagtca catgtccatg tccccggccg tcgtcaatgt caccctcctg atcaccgcct
     5701 cggccatcta tggcagcgtc ttctcgctga acaagctggc ggccgccgcg ggctggcagc
     5761 cgctgcagtt cgccttcgcc cagtcgctgc tcgccgggct cgccctggcc gtcgtcgtgc
     5821 tcgtccggcg cgagctgcca cggccgagcg tgcgcctgat cctcgcctgt ctcgtcatcg
     5881 gctcgctctt cgtcggtctg ccgatcacgc tcttgagcca tgtcgccccg catctgccgg
     5941 ctgccgtgac cacgctggtc ctcgccctct cgcccgtggt gacgctgatc ttcgccatgg
     6001 cgctcggcgt cgaacgcttc cgctgggggg ccgtcgtcgg catcgcctgc ggcctcgccg
     6061 gcatcgcggt cctggtcatg cccggctcct cgctcggtgg cggatcggcg gggagctggt
     6121 tcctcctggc cctcctcgca ccggtcatgt tcggcctcgg caacgtgctc tcgagcattc
     6181 tccgcccgcc ggtgaccgcc tcgaccgcga tggccggcgg ctcgctcctg ggctcggcgc
     6241 tgagcctcgt gccgctgctg gtggcaaccg gcctcttcgg ctggccggcg ctcggaccgg
     6301 cgaccctgcc gccggtgctg ctcgccagtg cggtgaacgc cgtcttcttc gtgctgctct
     6361 tcgagatcat ccgccgcgcc gggccgacct tcttcgccca gttcaactat ctcgccgtcc
     6421 tcgccggcgt cgcctggggc gcgatgctct tcgacgagcg gccggggttg agcttctggc
     6481 tggcgctggc tttgatgctg gccggcgtct tcttcgctac ccgtgctccc gagaaggctt
     6541 ccgcctgagc cggctcaggt gccgcagaag gtggcgagca cccgctcctc ggggcagggc
     6601 ggctccatgt aggccgcgca gcgtgcgccc ggatcgtcgt agggctgggc gagcgcttcg
     6661 agcagttccc ggaaggggcc gagatcgccg cgctcgacgg ccgcctcgag cgcctgctcg
     6721 acccggtggt tgcgcgggat gatcgccgga ttgacccggc gcatgagcgc cgaccgttcc
     6781 gccagggtcg tggattctcg ggtgagccgc acccgccagc cgacggccca attccgatac
     6841 agttccggcg tggcgaagag ctcggcgacc cggtcttcta gagccgggtc cagggccgca
     6901 tcggcgagcc ggcggaaggt gagcgtgtag tcggccgctc ccttgtgcat ggcgtccagc
     6961 aaggcgtgga cgagggcaag gtcgtccttc tccgccatct ctagcccgat cttccggcgc
     7021 atccgctgga ggagctcgct ctcgaacgtc cgaccgaaga cctcgaccgc ctcggtggcg
     7081 cgggtcacgg cgcgctcgac atcggagtcg atcaggccga gcagcgcctc ggcgaggcgg
     7141 gcgaggttcc agccggcgag gcgcggctgg ttggcgaagg catagcggcc ggcatggtcg
     7201 atcgagctga acacggtggc cgggtcgtag gcgtccatga aggcgcaggg gccgtagtcg
     7261 atggtctcgc cggcgataga catgttgtcg gtgttcatca cgccatggat gaagccgacc
     7321 gacagccaca tcgcaatcag cctcgcctgg cgctccgcca cggcggcaag gaggtccagc
     7381 gccggctggt cgctctggcc gagctcggga tagtggcgct cgatcacata ggcgacgagg
     7441 cgggccaagg cctcggtgtc gccgcgcgcc gcgaagaact ggaacgtgcc gacccgcaca
     7501 tggctgagcg ccacgcgggt gagcacggcg cccggcaggg tggtctcgcg atggaccggc
     7561 tcgcccgtgg tgacagcagc gagggcccgg gtcgtcggga tgccgagcgc gtgcatcgcc
     7621 tcgcccatca gatattcgcg cagcaccggg ccgagtgccg cgcggccgtc gccgccgcgc
     7681 gagaagggcg tacggccggc acccttgagc tggatgtcgc gacgctggcc agaacggtcg
     7741 ttgacctcgc cgagcaggat cgcccgaccg tcgccgagtt gcggcacgaa ctggccgaac
     7801 tgatgcccgg cataggccat ggcgagcggc tccgccccgt ccggcaacac gttgccggcg
     7861 agcatggccg ccccatcggc gctgtcgagc agctccggat cgacaccgag atcgcgggcg
     7921 aggtcgcggt tgacggcgac
//
LOCUS       DAOTFI010000223         7829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11165_length_7829_cov_5.518909, whole genome shotgun sequence.
ACCESSION   DAOTFI010000223 DAOTFI010000000
VERSION     DAOTFI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11165_length_7829_cov_5.518909"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="P5558_18995"
     CDS             complement(<1..1150)
                     /locus_tag="P5558_18995"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY26462.1"
                     /translation="MKITSVRVRQVTGTMATDGPFWEDRLLRPIDIYPEYRREMPPWG
                     GEQVDDTHFRLTQWFVVVDTDDGVSGTAGPLWPDAARLVLTQLRPLLLGRDPLATELL
                     WDQMHRSQVHGRQGDAMIALSAVDCALWDLKGKAMGQPVWRLLGGPTRSEVPAYASML
                     GYKVEDLGRVRERALEFKEQGYRAQKWFFRNGPMDGHAGMKLNVGLVRTLRETLGDDY
                     DIMLDCWQSMNLDYAVDLCSRIEEYRPRWLEEAFMPDRIDSYARLKARTRIPLSGAGH
                     EYTRWGFKRFVDQAALADDPALAWREGVQRLLEPARTGRGVAHPDHHLLGVGAGIGQE
                     VLPGVLAALAQRRAERLVGGGKAHLHRLHVGDRDFQRLGDPPADLGLGP"
     gene            complement(1147..1899)
                     /locus_tag="P5558_19000"
     CDS             complement(1147..1899)
                     /locus_tag="P5558_19000"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY26463.1"
                     /translation="MRQQAVELPRTAATDDGSPVRRVRRPRSLSAMVVNQLRELIIEG
                     QIDLGEQLSENVLAERLGVSRTPVREAFMKLAAERLVEVRPQRGTFVFTCTADEVREI
                     CELRAILESGALRLGAARDRAGLVTALARAADAAEPALLQSAQAYQPHDSAFHDTLMA
                     FAGNAHLVEAYQRIAGRVRTLRWRYTRTLDEVAESQANHREVIRHIAAGDDEAAEAAL
                     RDHVHKSHRGFQRRHEADAAAGITSSTRGGPA"
     gene            2062..3099
                     /gene="dctP"
                     /locus_tag="P5558_19005"
     CDS             2062..3099
                     /gene="dctP"
                     /locus_tag="P5558_19005"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY26464.1"
                     /translation="MNIRLSRALAVSAVAAWAVGAALGHAAEFTMKFSSATPETENSF
                     AWTHLKVLKQEIEARSGGQIEVEMYPGGQLGGIESLVNQVRDGIIQAADPADGHFAPL
                     FPDIQVFGIPYVFVSREVAWRVLDGPFGEMMRDKMAEATGLRPLFWTENGGFRHYSVV
                     GRELRSADDMAGIKMRTMNHPLHMEIASSLGMSPTPIAWGELYTALQTGVVEGQENSI
                     PTFLLPKLYEVQDNIILDGHVYSINTVVVNEAWYQSLPEDLRAAVDQAAVIARTTNRG
                     LSIANEIEGRAFLEEQGVTIYDPTAAEKAVFRERAQPPALEWLKTQIDPVLIDEMLAA
                     VAEAEEYYGYR"
     gene            3174..3665
                     /locus_tag="P5558_19010"
     CDS             3174..3665
                     /locus_tag="P5558_19010"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY26465.1"
                     /translation="MYDRVKRLVDGLSSAVMIVCNVVLVIMTGVIVMLVTTRNFMGFS
                     YAWSEELTRYLLVWLSMLGAAVLLRRDDHIALDLLPNALPSKARVGLVLALRLPVLAF
                     LALLLQQSWLTAIARATTRAPALGVSISWAYAAIPVMALLMLVFGAFNVWADLRRLRG
                     LDA"
     gene            3665..4960
                     /locus_tag="P5558_19015"
     CDS             3665..4960
                     /locus_tag="P5558_19015"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY26466.1"
                     /translation="MGIVEFIVLFLALLAVGLPIGFVMLLSSAAYFLLSGNPLFLRML
                     PEKMFAGIDVFVLMAIPFFLLTGEIMNRAQLTDRLFTFCNMLIGRLRGGLAQVNVLAS
                     VMFAGVTGVALGDVAALGKMFIPAMVRQGYDKPFTAAITAASSLIGAIIPPSTIIIIY
                     AAVMNVSVGAMFAAAIFPGLLLGVAMMGVVQWLSWKRQYPKVHVEVTPMGLAIGFRDA
                     FFAIIMPIIIIRGIIVGWFTPTEAAAICVVYSLVVGTLFLRTIRLADFPSILSTASLD
                     TARLFFIIAGASAVSWVFAMENMPALVTSLLAGVAEDRLTVVLIITAFFLFCGLWLDA
                     SISIILFAPVVLPLALKAGLHPVQLGIMLIVNCVLGLITPPVGNVLFVVSNVARLGIG
                     ALSRALVPFIGVGVAVVLLIGLWPDLTLALPRAFGLIRP"
     gene            4980..6140
                     /locus_tag="P5558_19020"
     CDS             4980..6140
                     /locus_tag="P5558_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26467.1"
                     /translation="MKISGVTIYDVDYGRDFHTNWNPVIVRIETDEGLHGAGEIGLAY
                     GDGAKGGIGMLKTFAERHLIGADPFRIEAIWDTLFRRTFWGQSAGPIVTGAISAIDEA
                     LWDIKGKALGQPVHALLGGKVRDEIRVYANGWYTGLVSPEDYAEGALKVVADGYDALK
                     LDPFGTTADGRWEYPRRHIDRDWAMLAVRRVEAIRKAVGDEVDILLDIHGNLGTTDAI
                     IYGRMLEASRPFFYEEPVDPMNAAAMKKVSDNVRIPIAAGERLYTRYQFRPYLESGAL
                     DILQPDMGLCGGITEARKIAAYAETYNLHVQPHNCGGPVSTAAGVQLDAVIPNFIIME
                     WFPYWTDGRYAIVTEALEPQQKKGRFRIPDRPGLGVELNDDYLARFDQIQVR"
     gene            6151..7317
                     /locus_tag="P5558_19025"
     CDS             6151..7317
                     /locus_tag="P5558_19025"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26468.1"
                     /translation="MKITKVTIFKTDFGASLNVHQGPITIRIDTDEGIYGAGEVGLAY
                     GDAQAGGIGMVEVLAERHLIGADPLRIEAMWDRLYRKTFWGQGAGPVLFGAISAIDEA
                     LWDIKGKALGVPVFELLGGRFRDDIRLYANGWNVAMRAPEEYAERVLQIAEEGYSAVK
                     LDPWERNLKGDFEFPPRHIPRDWAMLGVRRVEAIRKAVGDDLDICLDIHGNLGTSDAV
                     TYGRLLEASRPFFYEEPVETTNVDSLVKVMENVKIPIAGGERLYTRYQFRPYIERQAI
                     DILQPDINLTGGISETVKIAAMADVYNLHVQPHNCGGPVATAAAVQIAFAIPNFIIME
                     WFPYWPDGRYDIVTEAYETKMQRGRLAAPTAPGLGVELNDGYLARCDKVEIGGA"
     gene            complement(7320..>7829)
                     /locus_tag="P5558_19030"
     CDS             complement(7320..>7829)
                     /locus_tag="P5558_19030"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRY26469.1"
                     /translation="GMTAHGKIRVYAQKGEPIPEGWATDAQGNPTTDAVAALDGLILP
                     AGGHKGIGIGIMVGLLSTLLADAGYGLESGNMEEGAYVGRDGQFAMAIKIDAFRPAAG
                     VKARVDQIAREIRNSQKRPGVKALHPPGGLEATLEAAYRRDGIPLNDITLAGIADAAQ
                     ALGVAVDLG"
BASE COUNT         1155 a         2729 c         2611 g         1334 t
ORIGIN      
        1 cggggcccag gccgaggtca gccggcggat cgccgagacg ctgaaagtct cgatctccga
       61 cgtgcaggcg atggagatgc gccttgccgc cgccgaccag tcgctcagcg cgccgctggg
      121 cgagagcggc gaggactcct ggcaggacct cctgtccgat ccccgccccg accccgagca
      181 ggtggtgatc gggatgcgcg acaccgcgtc ccgttcgcgc tggctcaagg aggcgctgga
      241 cgccctctcg ccacgcgagc gccggatcat ctgcgagcgc cgcctggtcg acgaagcgct
      301 tgaagcccca gcgcgtgtac tcgtgcccgg cacccgagag ggggatgcgc gtcctggcct
      361 tgagcctcgc atagctgtcg atgcgatcgg gcatgaacgc ctcctcgagc cagcgcggcc
      421 ggtactcctc gatgcgcgag cagaggtcga cggcgtagtc gaggttcatg ctctgccagc
      481 agtcgagcat gatgtcgtag tcgtcgccga gggtctcgcg gagcgtgcgc acgaggccga
      541 cattgagctt catgccggca tggccgtcca tcgggccgtt tctgaagaac catttctgcg
      601 cccggtagcc ctgctccttg aattccagcg cccgctcgcg cacccggccg agatcctcga
      661 ccttgtagcc gagcatggag gcgtaggccg gcacctcgct tcgggtcggc ccgccgagca
      721 gccgccagac cggctggccc atggccttgc ccttgaggtc ccagagcgcg cagtcgacgg
      781 ccgagagggc gatcatcgcg tcgccctggc ggccgtgcac ctggctcctg tgcatctggt
      841 cccacaagag ctcggtggcg agcgggtcgc ggcccaggag gagcgggcgg agctgggtca
      901 ggacgaggcg cgccgcatcc ggccagagcg gtccggccgt gccgctgacg ccgtcgtcgg
      961 tgtcgacgac gacgaaccac tgggtcaggc ggaaatgggt gtcgtcgacc tgctcgccgc
     1021 cccagggcgg catctcccgg cgatactccg ggtagatgtc gatgggccgc aggaggcggt
     1081 cctcccagaa cggcccatcg gtcgccatgg tcccggtcac ctggcgcacc ctgacggatg
     1141 tgatcttcat gctgggcctc ccctggtcga gctggtgatg ccggcggccg catcggcctc
     1201 gtgccggcgc tgaaatcctc gatggctctt gtgcacgtgg tcgcgcagcg ccgcctcggc
     1261 cgcctcgtcg tcgccggcgg cgatgtgccg gatcacctcg cggtggttgg cctgcgattc
     1321 ggcgacctcg tcgagggtcc gggtgtagcg ccagcgcagc gtcctgacgc ggccggcgat
     1381 gcgctggtag gcctcgacca gatgggcgtt gccggcgaag gccatgagcg tgtcgtggaa
     1441 ggcgctgtcg tgcggctggt aggcctgggc gctctgcagc agggcgggct cggccgcgtc
     1501 ggcggcccgg gcgagcgctg tgactagccc ggcccgatcg cgggcggcac cgagccggag
     1561 cgcgccggat tccagaatgg cgcgcagctc gcagatctcg cgcacctcgt cggcggtgca
     1621 ggtgaagacg aaggtgccgc gctgcgggcg cacctcgacc aggcgctcgg ccgcgagctt
     1681 catgaaggcc tcgcggacgg gggtgcggct gacccccagc cgctcggcga gcacgttctc
     1741 ggagagctgc tcgccgaggt cgatctggcc ctcgatgatc agctcgcgga gctggttcac
     1801 caccatggcg ctgagcgagc gtggccgccg cacgcggcgc accggcgagc cgtcgtcggt
     1861 cgcggccgtc cgcggtagct ccactgcctg ctgcctcatc tgctcctgac catggccgag
     1921 ccgccgccgg gcatccggcg acccgacgct tcgacgtcat gactaacgca cttgtatgtt
     1981 acattcaagc cggctagggt cggcgcaacg agcagggagg atcatgtccg ccccgccgcc
     2041 atcgtcgaac gggaggtcga catgaacatt cgcttgtccc gcgcgctcgc cgtctctgcc
     2101 gtcgcggcct gggccgtcgg tgccgccctc ggccacgcgg ccgagttcac catgaagttc
     2161 tccagcgcca cgcccgagac ggagaacagc ttcgcctgga cccacctcaa ggtcttgaag
     2221 caggagatcg aggcccgctc cggcggccag atcgaggtcg agatgtatcc gggcggccag
     2281 ctcggcggca tcgaatcgtt ggtcaaccag gtgcgtgacg gcatcatcca ggcggccgac
     2341 ccggcggacg gccacttcgc cccgcttttc cccgacatcc aggtcttcgg cattccctac
     2401 gtcttcgtca gccgcgaggt cgcctggcgg gtgctcgatg gccccttcgg cgagatgatg
     2461 cgcgacaaga tggccgaggc gaccggcctg cgcccgctct tctggacgga gaacggcggc
     2521 tttcgccact actcggtggt cggtcgcgag ctgcgcagcg ccgacgacat ggccggcatc
     2581 aagatgcgga ccatgaacca tccgctgcac atggagatcg ccagcagcct cgggatgtcg
     2641 ccgaccccga tcgcctgggg cgagctctac accgccctgc agaccggcgt ggtcgagggt
     2701 caggagaatt cgatcccgac cttcctcctg ccgaagctct acgaggtgca ggacaacatc
     2761 atcctcgacg gccacgtcta cagcatcaac acggtggtcg tgaacgaggc ctggtaccag
     2821 agcctgcccg aggatctcag ggcggcggtc gaccaggcgg cggtgatcgc gcgcaccacc
     2881 aatcgcggcc tctcgatcgc caacgagatc gaggggcgcg ccttcctcga ggagcagggc
     2941 gtcaccatct acgacccgac ggcggccgag aaggcggtct tccgcgagcg cgcccagccg
     3001 ccggcgctgg agtggctgaa gacccagatc gatcccgtgc tgatcgacga gatgctcgcc
     3061 gcggtcgccg aggccgagga atattacggc tatcgctgag cgcgccgtga cgggcggcga
     3121 tcccttcgcc gggattgccg ccctgccgtt cccgtctccc ggagtccgtg accatgtacg
     3181 accgcgtcaa gcggctggtc gacggcctga gctcggccgt catgatcgtc tgcaacgtcg
     3241 tgctcgtgat catgacgggc gtgatcgtca tgctcgtcac gacccgcaac ttcatgggct
     3301 tctcctatgc ctggtcggag gagctgaccc gctacctcct ggtctggctg tcgatgctgg
     3361 gtgccgcggt tctcctgcgg cgtgacgacc acatcgccct cgacctgctg ccgaacgccc
     3421 tcccctccaa ggctcgcgtc ggcctcgtcc tggcgctgcg gctgccggtc ctggcctttc
     3481 tcgccctgct gctccagcaa tcctggctca cggcgatcgc ccgggcgacg acccgtgcgc
     3541 cggcgctcgg cgtctcgatc tcctgggcct acgccgccat tccggtgatg gccttgctca
     3601 tgctcgtctt cggcgccttc aacgtctggg cggatctccg tcggctgcgg ggcctcgacg
     3661 cctgatgggc atcgtcgagt tcatcgtcct gttcctggcc ctgctcgcgg tcggcctgcc
     3721 gatcggcttc gtcatgctgc tctcgtcggc cgcctatttc ctcctctccg gcaacccgct
     3781 cttcctgcgc atgctgccgg agaagatgtt cgccggcatc gacgtcttcg tgctgatggc
     3841 gatcccgttc ttcctgctca ccggcgagat catgaaccgg gcgcagctca ccgaccggct
     3901 cttcaccttc tgcaacatgc tgatcggccg cttgcggggc ggcctcgccc aggtcaacgt
     3961 gctggccagc gtgatgttcg ccggggtcac cggcgtggcg ctcggcgacg tcgcggcgct
     4021 cggcaagatg ttcatcccgg cgatggtgcg ccagggctac gacaagccct tcaccgccgc
     4081 catcacggct gcgtcgtcgc tgatcggcgc catcatcccg ccgagcacca tcatcatcat
     4141 ctacgctgcc gtgatgaacg tctcggtcgg cgccatgttc gccgccgcca tcttccccgg
     4201 gctgctcctc ggcgtcgcca tgatgggcgt ggtgcagtgg ctctcctgga agcggcaata
     4261 cccgaaggtc catgtcgagg tgacaccgat gggcctcgcc atcggctttc gcgacgcctt
     4321 cttcgccatc atcatgccga tcatcatcat tcgcggcatc atcgtcggct ggttcacgcc
     4381 gaccgaggcg gcggcgatct gcgtcgtcta ctcgctggtc gtcggcaccc ttttcctgcg
     4441 cacgatccgc ctcgccgact tcccctcgat cctctcgacc gcctcgctcg acacggcccg
     4501 gctcttcttc atcatcgccg gcgcctcggc ggtgagctgg gtcttcgcca tggagaacat
     4561 gccggcactg gtgacctcgc tcttggccgg ggtcgccgag gaccggctga cggtcgtgct
     4621 catcatcacg gctttcttcc tcttctgcgg gctctggctc gacgccagca tctcgatcat
     4681 cctcttcgcg ccggtcgtgc tcccgctggc gctcaaggcc ggcctgcacc cggtgcagct
     4741 cggcatcatg ctcatcgtga attgcgtgct cgggctgatc acgccgccag tcggcaatgt
     4801 cctcttcgtc gtctccaatg tcgcccggct cggcatcggc gccttgagcc gggcgctcgt
     4861 gccgttcatc ggcgtcggcg tcgccgtcgt gctgctgatc ggcctctggc ccgacctgac
     4921 gctcgctttg ccgcgcgcct tcggcctgat ccggccgtga cccaccaagg actcgaagca
     4981 tgaagatctc cggtgtcacg atctacgacg tcgactacgg ccgcgacttc cacaccaact
     5041 ggaacccggt gatcgtccgc atcgagaccg acgagggcct acacggcgcc ggcgagatcg
     5101 ggctcgccta tggcgacggg gccaagggcg gcatcggcat gctcaagacg ttcgccgagc
     5161 ggcatctgat cggcgccgat ccctttcgca tcgaggccat ctgggacacg ctcttccgcc
     5221 gcaccttctg ggggcagagt gccgggccga tcgtcacggg ggcgatcagc gccatcgacg
     5281 aggcactctg ggacatcaag ggcaaggcgc tcggccagcc ggtccacgcg ctgctcggcg
     5341 gcaaggtcag ggacgagatc cgggtctacg ccaatggctg gtacaccggc ctcgtctcgc
     5401 ccgaggacta cgccgagggg gcgctgaagg tggtcgccga cggctacgac gcgctcaagc
     5461 tcgacccctt cggcacgacg gccgacggca ggtgggaata tccccgccgg cacatcgacc
     5521 gcgactgggc gatgctcgcc gtgcggcgcg tcgaggcgat ccggaaggcg gtcggcgacg
     5581 aggtggacat cctgctcgac atccacggca atctcggcac caccgacgcc atcatctacg
     5641 gccgcatgct cgaggcgtcc cgccccttct tctacgagga gccggtcgat ccgatgaacg
     5701 cggcggcgat gaagaaggtc tcggacaatg tccgcatccc catcgcggcg ggcgagcgcc
     5761 tctacacccg ctaccagttc cggccctatc tcgagagcgg tgccctcgac atcctccagc
     5821 cggacatggg gctctgcggc ggcatcaccg aggcgcgcaa gatcgcggcc tatgccgaga
     5881 cctacaacct gcacgtccag ccgcacaatt gcggcgggcc ggtctcgacc gccgccggcg
     5941 tgcagctcga cgcggtgatc cccaacttca tcatcatgga gtggtttccc tactggaccg
     6001 acggccgcta cgcgatcgtc accgaagcgc tcgagccgca gcagaaaaag ggccgcttca
     6061 ggatacccga caggccgggg ctcggcgtcg agctgaacga cgactacctc gcccgcttcg
     6121 atcagatcca ggttcgctga gggccattcg atgaagatca ccaaggtcac catcttcaag
     6181 accgatttcg gcgcctcgct caacgtgcac cagggcccga tcaccatccg catcgacacc
     6241 gacgaaggca tctacggtgc cggcgaggtc ggcctcgcct atggcgacgc ccaggcgggt
     6301 ggcatcggca tggtcgaggt cctggccgag cgacatctga tcggcgccga tcccctgcgc
     6361 atcgaggcga tgtgggatcg gctctatcgc aagaccttct ggggccaggg ggcagggccg
     6421 gtcctgttcg gtgcgatcag cgccatcgac gaagcgctct gggacatcaa gggcaaggcg
     6481 ctgggcgtgc cggtcttcga gctgctgggc ggccgttttc gcgacgacat ccgcctctac
     6541 gcgaacggct ggaacgtggc gatgcgcgca cccgaagaat atgccgagcg ggtgctgcag
     6601 atcgccgagg agggttacag tgccgtcaag ctcgacccct gggagcgcaa tctcaagggc
     6661 gatttcgagt tcccgccgcg ccatatcccg cgcgactggg ccatgctcgg cgtgcgccgg
     6721 gtcgaggcga tccgcaaggc ggtcggcgac gatctcgaca tctgcctcga catccacggc
     6781 aatctcggca ccagcgacgc cgtcacctat ggccgcctgc tcgaggcatc caggcctttc
     6841 ttctacgagg agccggtcga gacgaccaat gtcgattcgc tcgtgaaggt catggagaac
     6901 gtgaagatcc cgatcgccgg cggcgagcgg ctctacacgc gctaccagtt ccgcccctat
     6961 atcgagcgtc aggcgatcga catcctgcag ccggacatca acctgaccgg cgggatcagc
     7021 gagaccgtca agatcgcggc catggccgat gtctacaacc tgcacgtcca gccgcacaat
     7081 tgcggcggcc cggtggcgac ggcggcggcc gtgcagatcg ccttcgcgat ccccaacttc
     7141 atcatcatgg agtggttccc ctactggccg gacggccgct acgacatcgt caccgaggcc
     7201 tacgagacga agatgcagcg ggggcggctc gcagcgccga cggcccccgg gctcggcgtc
     7261 gagctcaacg acggctatct cgcacggtgc gacaaggtcg agatcggcgg agcctgacct
     7321 cagccgaggt cgaccgcaac gcccagcgcc tgcgccgcat cggcgatgcc cgcgagggtg
     7381 atgtcgttga gggggatgcc gtcccggcgg taggcggcct cgagggtggc ttcgaggccg
     7441 cccggggggt ggagggcctt gacgccgggg cgtttctggc tgttccggat ctcgcgggcg
     7501 atctggtcga cgcgggcctt gacgccggcg gcggggcgga aggcgtcgat cttgatcgcc
     7561 atggcgaact ggccgtcgcg gccgacatag gcgccctcct ccatgttgcc ggattcgagg
     7621 ccgtagccgg cgtcggcgag gagggtcgag aggaggccca ccatgatgcc gatgccgatg
     7681 cccttgtggc cgccggcggg gaggatgagg ccgtcgagcg cggccacggc gtcggtcgtg
     7741 gggttgccct gggcgtcggt cgcccagcct tcggggatgg gctcgccttt ctgggcgtag
     7801 acgcggatct tgccgtgcgc ggtcatgcc
//
LOCUS       DAOTFI010000224         7785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11247_length_7785_cov_6.947995, whole genome shotgun sequence.
ACCESSION   DAOTFI010000224 DAOTFI010000000
VERSION     DAOTFI010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11247_length_7785_cov_6.947995"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="rfbA"
                     /locus_tag="P5558_19035"
     CDS             complement(<1..794)
                     /gene="rfbA"
                     /locus_tag="P5558_19035"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRY26470.1"
                     /translation="MPNPTRQPRWKGIILAGGSGTRLYPITRAVCKQLLPIFDKPLIY
                     FPLTTLMLAGIRDILLITTPEDQWQFKSLLGDGSQWGLAISYAVQPRPEGLAQAFVIG
                     REFIGDDNVALVLGDNIFFGHYLRADLARAIDQAHGATVFGYRVTDPERYGVVAFDEA
                     GRATSIEEKPARPRSSYAVTGLYFYDNEVIDIAAGLPPSARGEYEITDVNAAYLARGA
                     LRVERLGRGYAWFDAGTHDSLLQAGEFVRTIQQRQGFQIACPEEIA"
     gene            complement(889..1941)
                     /locus_tag="P5558_19040"
     CDS             complement(889..1941)
                     /locus_tag="P5558_19040"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY26471.1"
                     /translation="MERVLVTGAGGYIGTTLVPMLLAQGYQVRALDRFFFGAELLAPH
                     ARLELVKADSRRLEAHHFKGVDHVIDLVAISNDPSGELFQDATYAINHESRVHCARLA
                     KMAGVKRYVLPSSCSLYGFQPEDVICAEDHATNPLTTYARANEMAEHGVLPLADERFC
                     VVVLRQATVYGYSPRMRFDLAINGMTLGAWQTGTLPLMRDGTQWRPMVHVRDTAAAQI
                     FMLEAPAASINGQIFNVGSDANNYQLGPLAERIAAAVPRDVAVEWYGAADHRSYRVAF
                     AKIEKLGWHAQRVAEDGVAEIVAKLVDGSLEKSTKTITLDWYQELIKWQRIIHEVELD
                     GTIFDLPDHRSKAASG"
     gene            complement(1954..2541)
                     /gene="rfbC"
                     /locus_tag="P5558_19045"
     CDS             complement(1954..2541)
                     /gene="rfbC"
                     /locus_tag="P5558_19045"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRY26472.1"
                     /translation="MEIVATAIPEVKLIRPKRFGDHRGFFAEVYNRRAFEAAGLRFDF
                     VQDNHSRSAQRGTLRGLHYQSPPFAQTKLVRVLKGRILDVAVDIRQGSPTYGRHVAVE
                     LSAEGFEQLLVPRGFAHGFVTLEDEVEVFYKVDAYYSAAHDHGIAWNDPDLAIDWPVS
                     PVECMLSAKDAAQPAFAGLAAHFTFVPAAPAATTV"
     gene            2699..4786
                     /locus_tag="P5558_19050"
     CDS             2699..4786
                     /locus_tag="P5558_19050"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRY26473.1"
                     /translation="MTDAAPPRAAQHPIVNERHGVSWTDPYAWLRDPAYPEVQDPEIR
                     AYLEAENAYFAAVMAREQGLIQELHTELKGRIKDDDSSVPVADGAYLYHWQFQPGAQY
                     RTWYRAPREGGTAAVMLDETALALDKSYFNLRSLAPSPDGQLLAFATDENGSERYVIH
                     IKDLASGVSTTDLVANTAGSVVWSADGATLFYVELNEKLRPYRVRAHRLGTDATADRT
                     VYTEDDPAFFVSIGLTQSRRFVVVTAGSHVTREVRLIDATRPAGEPLLIAGRRDGHRY
                     SVEHAHDRLWIRTNDRHENFRLVSAPIDAPGEASWQEEIAGDDHHYLLGVTCFRDFLV
                     ITERLDGLANLRIRDYAGEEHAIDFAEAVYTAGLGDNREFATDRLRVGFSSLTRPATV
                     LDYHVADRRLEVLKEQEIPSGYDASRYVGRRLWAAAADGERVPISLVHARDCPVDGSG
                     RLYLYGYGSYGMAMEPSFNSTRLSLLDRGFAVAIAHVRGGDELGHRWYREGKLERKTN
                     SFTDFIACAEALIEGGYAAPGRIAIRGGSAGGMLMGAVTNLRPELWRCVVAEVPFVDV
                     LNTMLDASLPLTPIEWPEWGNPLTSEEDFRRIRAYCPYENIEVKAYPAMLVTAGISDP
                     RVTYWEPAKYVARLRELKTDRNLLLLKTNMDAGHFGASGRFDALKELAEILAFILKCF
                     RDGDGPGEASAGG"
     gene            complement(4806..7655)
                     /locus_tag="P5558_19055"
     CDS             complement(4806..7655)
                     /locus_tag="P5558_19055"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRY26474.1"
                     /translation="MPGKAKGKRLSKAERALIRDVLGWRERYDDRPTRDRAGAAPAAT
                     APRVLAAEASALIVRHESGGRAYYEQVIKKRPIWPEFSSGLTIGFGFDIGHRSKAELE
                     RVWTPHLGAEAVARLARAVGLTATGADREAKVARLKKLAKELADIEVPWSMAEAVFAA
                     EEVPIQVAKTTAALPNTELLGDTCLGALVSLVFNRGSGGFTSSKTRFREMRAIRQHMA
                     DRNFAAIPAEIRSMCRLWPDHPGLTGRREEEAALFEAGLASLPATAAARPAPAWPALV
                     ASLDAAPDRFDTRDLPYRPPLVSLPPSWPPALWIERFLPQYAADGMVLDQRAEGACTG
                     FGLAAVINYIAWEAWQRATIEERPAPAPVKVSERMLYHMAQLYDEWEGEDYEGSSCRG
                     AMKGWHKHGVCAGHLWPYEDGSFVGPDPTWASDAALRPIGAYYRIDRSSLVDLQAAIA
                     EVHAIYVSAQVHGGWNLGPAADAPPVIDWNGEAAAGAHAFALVGYDERGFIVQNSWGP
                     DWGFGGFAILTYGDWLAHGHDAWVAVLGAPIGRTTSPVALSEAPLQARAATLAGAGAG
                     RADSAAPPAAAPWAADTALERTLVLGNDGRPLHRLLTARDAAANLACVVEEHLLRRLR
                     DSGSRKLVVYAHGGLNDEPASLLRVRRLGPYFEANGVPALFFTWRTGVRDCLLGILED
                     AASRIGIDLALVRAEGLARGLKEELAETRDATLEVLSERLLGKAVWTQMKQNAAAASE
                     PAGGAALAARSLARLAGEIEGLEIHLIGHSAGAIFHGHLLRRLAAEGLAVKSAELWAP
                     ACSLRFAVERYGRAFAEGTLAPKALHIALLADVIERADSVGPYGKSLLYLVSRAFEPL
                     RRMPLLGLADAWTGDVPVAARPFNSAARADIEAWRALWRGRLELVSEPEVGDGRKTIP
                     AVHGSFDNNAALVSATIRRIRGQKLALPVGDLGGF"
BASE COUNT         1203 a         2689 c         2723 g         1170 t
ORIGIN      
        1 tgggcgatct cctccgggca ggcgatctgg aagccctggc gctgctggat ggtgcgcacg
       61 aactcgccgg cctgcaggag gctgtcgtgg gtgccggcgt cgaaccaggc atagccacgg
      121 cccagccgct cgacgcggag cgcgccgcgg gcgagatagg cggcgttgac gtcggtgatc
      181 tcgtactcgc cacgcgctga gggtggcagg ccggcggcga tgtcgatcac ctcgttgtcg
      241 tagaaataga ggccggtgac cgcatagctc gacctcggcc gggccggctt ctcctcgatg
      301 ctggtcgccc ggccggcctc gtcgaacgcc accacgccgt agcgctccgg atcggtcacc
      361 cgatagccga agaccgtggc accatgggcc tggtcgatcg cgcgggccag atcggcgcgc
      421 agatagtggc cgaagaagat gttgtcgccg agcacgaggg cgacgttgtc gtcgccgatg
      481 aactcgcggc cgatgacgaa ggcctgcgcc aggccctctg ggcgcggctg caccgcatag
      541 ctgatggcca ggccccactg gctgccgtca ccgagcaggc tcttgaactg ccactggtcc
      601 tcgggcgtgg tgatcaagag gatgtcgcgg atgccggcca gcatcagcgt cgtcagcggg
      661 aagtagatga gcggcttgtc gaagatcggc aggagctgct tgcagacggc acgggtgatc
      721 gggtagaggc gcgtgccgga gccgccggcg aggatgatgc ccttccatct cggctgcctg
      781 gtcgggttcg gcatcgcgtt gctccgtctc tcggtcgagg gttcggcggc gagcgccgcc
      841 cgaatggcat tcgtcaggct cggctcgccg acccgtgcgg cggttccttc agcctgaagc
      901 ggccttcgag cggtggtcgg gcagatcgaa gatcgtgccg tcgagctcga cctcatggat
      961 gatccgctgc cacttgatca gctcctggta ccagtcgagc gtgatcgtct tcgtcgactt
     1021 ctcgaggctg ccgtcgacga gcttcgccac gatctccgcg accccgtcct cggcgaccct
     1081 ttgggcatgc cagccgagct tctcgatctt ggcgaaggcg acccgatagg agcggtggtc
     1141 ggcagcaccg taccattcga cggcgacgtc acgcggcacg gcggcggcga tccgctcggc
     1201 gagcgggccg agctggtagt tgttggcgtc cgagccgaca ttgaagatct ggccgttgat
     1261 ggaggctgcc ggtgcctcga gcatgaagat ctgggcggcg gccgtgtccc tcacatggac
     1321 catgggccgc cactgcgtgc cgtcgcgcat caggggcagc gtgccggtct gccaggcgcc
     1381 gagggtcatg ccgttgatgg cgaggtcgaa gcgcatgcgc gggctgtagc cgtagaccgt
     1441 cgcctggcgc agcacgacca cgcagaagcg ctcgtcggcg agcggcagca cgccgtgctc
     1501 ggccatctcg ttggccctgg cgtaggtggt gagcgggttg gtcgcgtggt cctcggcgca
     1561 gatcacgtcc tcgggctgga acccgtagag gctgcaggag gacggcagga catagcgctt
     1621 gaccccggcc atcttggcga ggcgggcgca gtgcacccgc gattcgtggt tgatggcgta
     1681 ggtggcgtcc tggaagagct cgccgctcgg gtcgttggag atggcaacga ggtcgatgac
     1741 gtgatcgacg cccttgaaat ggtgcgcctc gagccgccgg ctgtcggcct tgaccagctc
     1801 gagccgggca tgcggcgcca gcagctcggc gccgaagaag aagcggtcga gggcgcggac
     1861 ctgatagccc tgggcgagca gcatcggcac gagggtggtc ccgatatagc cgccggcacc
     1921 ggtgacgagg acgcgttcca tcgagagctc cgatcagacg gtggtggctg cgggcgcagc
     1981 cggcacgaag gtgaaatggg cggcaaggcc ggcgaaggcg ggctgcgccg cgtccttggc
     2041 agagagcatg cactcgaccg ggctgaccgg ccagtcgatg gcgaggtcgg ggtcgttcca
     2101 ggcgatcccg tgatcatgcg ccgcactgta gtaggcgtcg accttgtaga agacctcgac
     2161 ctcgtcctcg agggtgacga agccgtgcgc gaagcccctg gggaccagga gctgctcgaa
     2221 gccctccgca ctcagctcga ccgcgacatg gcggccatag gtcggcgagc cctggcggat
     2281 gtcgaccgcg acgtcgagga tccggccctt cagcacgcgc accagcttgg tctgggcgaa
     2341 gggcggcgac tggtagtgca ggccgcgcag cgtgccccgc tgggcggagc gcgaatggtt
     2401 gtcctggacg aaatcgaagc gcagccccgc cgcctcgaag gcccggcggt tgtagacctc
     2461 ggcgaagaat ccgcgatggt cgccgaaccg cttcggccgg atcagcttca cctcgggaat
     2521 ggccgtggcg acaatctcca tgcgcacctc gcaaatgacc agcggtcgtg cggcttgcgg
     2581 cccgaccggc tcactgcggc gaacctacca gccgcccctt actctttcgt taatgcgcgt
     2641 ggcctaccgt tgcggccgcc gatcgggtag aagcgggcgg tcccgcagga gatctcgcat
     2701 gaccgacgca gcaccgccgc gtgccgcgca gcaccccatc gtcaacgagc ggcacggcgt
     2761 cagctggacg gacccgtatg cctggctccg cgacccggcc tatcccgagg tccaggaccc
     2821 cgagatccga gcctatctcg aggccgagaa cgcctatttc gccgccgtca tggcgcgcga
     2881 gcaggggctg atccaggagc tgcacaccga gctcaagggg cggatcaagg acgacgacag
     2941 ctcggtgccg gtggccgacg gcgcctatct ctaccactgg cagttccagc cgggtgcgca
     3001 gtaccggacc tggtatcgag caccgcggga gggcggcaca gccgcggtca tgctcgacga
     3061 gaccgcgctc gccctggaca agagctactt caacctgcgc agcctggcac cgagccccga
     3121 cggccaactg ctcgcctttg cgaccgacga gaacggctcc gagcgctatg tgatccacat
     3181 caaggatctc gcgagcgggg tcagcaccac cgatctcgtc gccaacaccg ccggcagcgt
     3241 cgtctggtcg gccgatgggg ccacgctctt ctatgtcgag ctcaacgaga agctgcgacc
     3301 ctaccgggtc agggcgcatc ggctcggcac cgacgccacg gccgaccgca cggtctacac
     3361 cgaggacgac ccggccttct tcgtcagcat cggcctgacc cagagccgcc gcttcgtcgt
     3421 cgtcacggcc ggcagccacg tcacgcgcga ggtcaggctg atcgatgcga cccgaccggc
     3481 gggcgagccc ttgctgatcg cgggccggcg ggacgggcat cgctacagcg tcgagcacgc
     3541 ccacgaccgc ttgtggatcc gcaccaacga ccggcacgag aacttccgtc tggtatcggc
     3601 accaatcgat gcccccggcg aggcaagctg gcaggaggag atcgcgggcg acgaccacca
     3661 ctacctgctc ggcgtcacct gctttcggga cttcctcgtc atcaccgagc ggctggacgg
     3721 cctcgccaac ctgcgcatcc gcgactatgc gggcgaggag cacgccatcg atttcgccga
     3781 ggcggtctac acggcggggc tcggcgacaa ccgcgagttc gccacggatc ggctcagggt
     3841 cggcttcagc tcgctcaccc ggcccgccac cgtgctcgac taccatgtcg ccgatcgaag
     3901 gctcgaagtg ctgaaggaac aggagatccc ttcgggctac gatgcgagcc gctatgtggg
     3961 ccggcgcctc tgggccgcgg cggcggacgg tgagcgggtg ccgatctccc tcgtccatgc
     4021 ccgtgactgc ccggtcgacg gcagcggcag gctctacctc tacggctacg gctcctacgg
     4081 catggcgatg gagccctctt tcaacagcac gcggctctcc ctcctggacc gcggcttcgc
     4141 ggtagccatc gcacacgtgc ggggcggcga cgagctcggc catcgctggt accgcgaggg
     4201 caagctggag cggaagacca acagcttcac cgatttcatc gcctgcgccg aggcactgat
     4261 cgagggcggc tacgcggcac cgggccggat cgccatccgc ggcggctcgg ccggcggcat
     4321 gctgatgggt gccgtcacca atctccgccc cgagctctgg cgctgcgtcg tcgccgaggt
     4381 accgttcgtc gacgtgctca acaccatgct cgacgccagc ctgccgctca ccccgatcga
     4441 gtggccggaa tggggcaacc cgctgacctc ggaggaggat ttccggcgca tccgggccta
     4501 ctgcccgtac gagaacatcg aggtcaaggc ctatcccgcc atgctggtca ccgccggcat
     4561 cagcgatccc cgtgtgacct actgggaacc cgcgaaatac gttgcccggc tgcgcgagct
     4621 caagaccgac cggaatctgc tcctcttgaa gaccaacatg gatgccgggc atttcggcgc
     4681 ctcgggccgg ttcgacgcgc tgaaggagct ggccgagatc ctggccttca tcctcaaatg
     4741 cttccgcgac ggggacgggc cgggcgaggc atcggccgga ggctgagagc agcgggcggc
     4801 gtcgctcaga agccgccgag atcgccgact ggcaaggcga gcttctgtcc cctgatccgg
     4861 cggatcgtgg ccgagaccag ggcggcattg ttgtcgaagg agccgtgcac cgccgggatg
     4921 gtcttcctcc cgtccccgac ctcgggctcg ctgaccagct cgagccggcc gcgccagagg
     4981 gcacgccacg cctcgatgtc ggcccgggcg gcgctattga atggccgcgc ggccaccggc
     5041 acgtcgcccg tccacgcatc cgcgagcccg agcagcggca tgcgcctgag cggctcgaag
     5101 gcgcgactga ccagatagag caatgacttg ccgtaaggcc cgaccgaatc cgcccgctcg
     5161 atgacatcgg cgaggagtgc gatgtgcagc gctttcggcg ccagggtgcc ttcggcgaag
     5221 gcccggccat accgctcgac ggcgaagcgc aggctgcagg ccggtgccca gagctcggcg
     5281 ctcttcacgg cgagaccctc ggcagcgagg cggcggagca gatggccgtg gaatatggca
     5341 cctgccgagt ggccgatcag gtggatctcg aggccctcga tctcgccggc cagccgggcc
     5401 agcgatctgg cggccagcgc cgcgccgccg gccggctcgg aagcggccgc cgcgttctgc
     5461 ttcatctgcg tccagaccgc cttgccgagc aggcgctcgc tcagcacctc gagcgtggcg
     5521 tcccgcgtct cggcgagctc ttccttcaga ccccgggcga ggccctcggc ccggaccagc
     5581 gccaggtcga tgccgatgcg ggatgcggca tcctccagga tgccgagcag gcagtcgcgc
     5641 acgccggtcc gccaggtgaa gaagagggcg ggcaccccat tggcctcgaa atacgggccg
     5701 agcctgcgaa cccgcaggag cgaggccggc tcgtcattga gcccgccatg ggcatagacc
     5761 acgagcttgc ggctgccgct gtcacggagc cggcggagca ggtgctcctc gacgacgcag
     5821 gccagattcg ccgccgcgtc gcgggccgtc agcagccggt gcagcggcct tccgtcattg
     5881 ccgaggacca gcgtccgctc gagcgccgta tcggccgccc agggtgccgc cgccggcggt
     5941 gcagcgctgt cggcgcggcc agcgccggct ccggcgagcg tcgcggcgcg ggcctgcagg
     6001 ggcgcctcgc tcagggcgac ggggctggtc gtccggccga taggtgcgcc cagcacggcg
     6061 acccaggcat cgtggccatg cgccagccag tcgccatagg tcaggatggc gaaaccgccg
     6121 aagccccagt cgggacccca cgagttctgc acgatgaagc cgcgctcgtc atagccgacg
     6181 agcgcgaagg catgggcacc cgccgccgcc tcgccgttcc agtcgatgac cggcggtgcg
     6241 tccgcagccg gcccgagatt ccagccgccg tgcacctggg ccgagacata gatcgcgtgc
     6301 acctcggcga tcgccgcctg caggtcgacg agcgagctcc gatcgatccg gtagtaggcg
     6361 ccgatcggcc gcagcgccgc gtcgctggcc catgtcgggt ccggtcccac gaagctgcca
     6421 tcctcgtaag gccagagatg gccggcgcag acgccgtgct tgtgccagcc cttcatagca
     6481 ccccggcagc tcgagccctc gtagtcctcg ccctcccatt cgtcgtagag ctgggccatg
     6541 tggtagagca tgcgctcgct gaccttgacc ggtgccggcg ccggccgctc ctcgatggtg
     6601 gcgcgctgcc aggcctccca ggcgatgtag ttgatgacgg cagcgaggcc gaagccggtg
     6661 caggcaccct cggcccgctg gtccagcacc atgccgtcgg ccgcgtattg cggcaggaag
     6721 cgctcgatcc agagcgctgg cggccaggac ggcggcagcg agacgagcgg cggccggtag
     6781 ggcaggtcgc gggtgtcgaa gcggtcgggc gccgcatcga ggctggcgac cagcgccggc
     6841 caggcgggag ccggcctggc cgcggccgtg gcgggcaagg acgcgagccc ggcctcgaag
     6901 agcgccgcct cctcctcgcg ccgccccgtc agccccgggt gatcgggcca gagccggcac
     6961 atggagcgga tctcggccgg aatggcggcg aagttgcgat cggccatgtg ctggcggatc
     7021 gcccgcatct cgcgaaaccg cgtcttgctg ctggtgaagc cgccactgcc gcggttgaag
     7081 accagcgaga ccagggcccc caggcacgtg tcgccgagga gctcggtatt gggcagggcg
     7141 gccgtggtct tcgccacctg aatcggcacc tcctcggccg cgaagaccgc ctcggccatg
     7201 ctccagggca cctcgatgtc ggcgagctcc ttcgccagct tcttcagccg ggcgaccttc
     7261 gcctcgcgat cggcgccggt cgccgtgagc ccgaccgccc gggcgagccg agccacggcc
     7321 tccgccccca gatgcggcgt ccagacccgc tcgagctcgg ccttcgagcg gtggccgata
     7381 tcgaagccga agccgatggt caggccggag ctgaattcgg gccagatcgg ccgcttcttt
     7441 atgacctgct cgtaataggc gcgcccgccg ctctcgtggc ggacgatcag ggcgctggcc
     7501 tccgccgcga gcacgcgggg tgccgttgcc gccggtgcgg cgccggcccg gtcacgcgtc
     7561 gggcggtcgt catagcgctc gcgccagccg agcacgtccc ggatcagggc ccgctcggcc
     7621 ttggacagtc gttttccctt cgccttgcct ggcacggcag cctcccccgc ctgtggccgg
     7681 tcccgactgg ccttcatcct accacaggtt gcggacgcgt gctcggggca gctcagagcc
     7741 tgtgaatgaa tgtcctgcgc cttttcgata gaatagccgc agcga
//
LOCUS       DAOTFI010000225         7756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11287_length_7756_cov_7.012726, whole genome shotgun sequence.
ACCESSION   DAOTFI010000225 DAOTFI010000000
VERSION     DAOTFI010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_7756_cov_7.012726"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="P5558_19060"
     CDS             <1..996
                     /locus_tag="P5558_19060"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY26475.1"
                     /translation="VAYCTSGTEATLHAVRLARAVTGRRRIIKFQGTYHGWHDYVAMN
                     WVSPQERLGGADPFTAGALPEAMAATTILPFNDLSAIEAVLDAADHDVAAVLVEPLMH
                     NIGCIPFEPGYAEALKALCERRGVLLIFDEIITGFRHALGGYQSLIGVTPDLATFGKA
                     IGNGHPLSAIVGRRDLLERFAAAPPHGVALGGTFNAHPRSVAAALATIERMAEPDAYP
                     ALYAKGEALAAALERAVAASGLDAVVTRFGSVVALHFQAEAPRRYEDLVHGDLARDAA
                     FRRGLLDHGIATAAQPLRRFCVSLAHEDHHMARIEEAAHAVLKDLARQGAGAAPA"
     gene            993..1772
                     /locus_tag="P5558_19065"
     CDS             993..1772
                     /locus_tag="P5558_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY26476.1"
                     /translation="MTVPAARLLSLDGRVALVTGASRGIGLAIAEALASAGAHVVLNA
                     RNAGRLDAAVAAIRTSSGSAEAAAFDVRDAGAVTDAVANIGDRLGRLDILVSNVGGGV
                     RKPLAEQTDVDFTAAIDTNLVACFRLARESSRLMVPQGFGRILATSSINSFVARPTMA
                     GYAAAKAGLNGLVRALAVELAPAGICVNALAPGYFPTEANRTLRATNPDLVAQVEART
                     PMRRWGAVEELGAAAIFLCSPGASYCTGQVLAVDGGLLASL"
     gene            1785..3257
                     /locus_tag="P5558_19070"
     CDS             1785..3257
                     /locus_tag="P5558_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRY26477.1"
                     /translation="MRIAVLNIGQESNDFNPLPTTLQDYRSFGLYEGQEMLEKLRGLG
                     EVGGYLEAIEESGLEVETIPIIRGWASAAGRLDDATRLFFEEKIRSGLEAAGRIDGLA
                     IQLHGACAADGMDDVEGAQLALCREILGPDVPIVLSLDHHANVTRQMVENADAVVGHR
                     TQPHQPFETGKIAAGILLRMLQGEVRPTVAMRKLRLLSHQEQFLTSKPPMKTWFDRAR
                     EMEQDPRVLQASPFPMQPWLDVAEGGWAVVVTTDGDRELAGRLADELADLAWSMRAEF
                     QKKDAVGVDEAVRRADAMDGLVILSDTGDTVFGGAAGDSNLILESILRQGIEGRALVP
                     MIAPEAVAKLAKAGVGAEVTLPLGGHATGFFEPLTVTGTVRAVGGGVIPVAKHNQLEV
                     DLGQAVIFEVGPVTMLLTELRAVAGNLPEPYRALGVQPEDYQMVVLKTASNFQYFQPI
                     ASGVIRVDTKGPGQSDIATLPWQRQPRPVYPLDEIDSWRG"
     gene            3366..4907
                     /locus_tag="P5558_19075"
     CDS             3366..4907
                     /locus_tag="P5558_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26478.1"
                     /translation="MSIQRRRLLSTTVALSLGVFMASGINATAEAQDGGTLRVAILQD
                     VSNFDPQSFLSLNFPLIKNLYDSLLEYGPDGEVLPSLASAWTIADDNMSVSLTLRDDV
                     TFHSGAPMDAEAVAATLAKAADPERGQNVYATMAPVENWTVDGPHQLTVNFKGPIPDR
                     QSTDLLQFISVIDPAGIETVATVPAGTGAFTLVERVLGQSVQMAANPNYWRDGEPKVD
                     ELLITVFGDNDAASAALESDAVDMIYGAGGRAAVRLRDSGYQLVQGPGPLVQVFRINT
                     TRPPFTNEKFRQAFKYLVDREAILRVGYAGLGVVTALPWAPASPAADPSYNETYAFDL
                     DKAKELLDASGLTPAEMSDWQLLVNGADQDAVTISQLVQGTLAELGINVELDLRQGAE
                     FTDAMLGGDYTAIFAGIGNIQKFPSRIATNSIYRTSNNPILGEPHPHPDYVAAIERVN
                     TTLGSDEEVQAAYDHLNEVLIEAAFGVPTNTYDVGLIVAAPDVSGFTPDIDNMLVART
                     ITVAR"
     gene            4956..7004
                     /locus_tag="P5558_19080"
     CDS             4956..7004
                     /locus_tag="P5558_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26479.1"
                     /translation="MAEPLLSIRGLEVTFRGTGRPVPAVRGIDLDVAADEVLGIVGES
                     GSGKSVTSLAMTGLLPSTAVVGGSIRIGGVEVTGADPEVLRQMRGRDVGMIFQDPTTS
                     LNPVLPIGRQVTEGQVAHGLLRPAEASGRAVELLAEVDIPDPAGRALQFPHQFSGGMR
                     QRAVIAMAMAGRPRLIIADEPTTALDVTVQAQVLTLLRRRQAATGAAVILITHDLGVI
                     AETAHRVVVMYAGKIVEAAPVAEIFARPRHPYTVGLLKSLPRLDQDTRRLVSIQGQPP
                     TPGAIPSGCAFHPRCPIGAARERCRSEEPRLRAAGPEGIAACHFTDEIEGLLAPPPEA
                     GGSASAPRAAPAEPLLVVDSLKVHFPIRRGLLRRQTGLVRAVDGVSLEVRAGETLGLV
                     GESGCGKTTTGRAIMGLIEASGGSIDFAGQDITQLDAAGLRAVRRHVQYVFQDPYASL
                     NPIRTVEAIVAEPLHIHGVFDAMGGNARVGWLLDLVGLSRTMTGRYPRELSGGQRQRV
                     GIARALALEPRLLILDEPVAALDVSIQAQVVNLLMDLQVELGLAYLFIAHDLSVVRHI
                     SDRVAVMYLGRIVEEGTRDQLYARPVHPYTQSLLSAVPVPEPGNAGRRIVLEGDIPNP
                     ADPPGGCAFHPRCYRATERCTREAPAFAAYPGLDTRVACHHAGPLTADELPAAAEVLT
                     "
     gene            7001..>7756
                     /locus_tag="P5558_19085"
     CDS             7001..>7756
                     /locus_tag="P5558_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26480.1"
                     /translation="MSASQRPALVAARRVLAQKSAFMAAVFLLGMALIAFLAPWILPG
                     DPLTQNLRHALEPPSAAFWFGADELGRDILTRVVYGARTSLVTALGAVAIAGLIGVPI
                     GLVAGFYGGWRDAVLMRLVDVILALPGILFAMALIAVIGRSQMAALVAVGITGIPSFA
                     RITRAQVLSLRRRDFVIAVDAMGGSASHLMFRTILPNSWSPILVQIVILAAVAILLEA
                     ALAFLGVGIPPPTPSWGEMLRTGKSYLYEAPYYA"
BASE COUNT         1102 a         2930 c         2547 g         1177 t
ORIGIN      
        1 gtcgcctact gcacctccgg caccgaggcc acgctgcatg ccgtgcggct cgcccgggcc
       61 gtgaccgggc ggcggcggat catcaagttc cagggcacct atcacggctg gcacgactac
      121 gtcgcgatga actgggtgag tccgcaagag cggctcggcg gtgccgatcc gttcaccgcg
      181 ggtgccctcc ccgaagccat ggcggcaacc accatcctgc ccttcaacga cctttccgcc
      241 atcgaggcgg tgctcgacgc cgccgaccac gacgtcgccg ccgtgctggt cgagccgctc
      301 atgcacaata tcggctgcat ccccttcgaa cccggctatg cggaagcttt gaaggcgctc
      361 tgcgagcggc ggggcgtgct cctgatcttc gacgagatca tcacgggctt tcgccacgcg
      421 ctcggcggct accagtcgct catcggcgtc acgccggacc tcgccacctt cggcaaggcg
      481 atcggcaacg gccatccgct ctccgccatt gtcggccggc gcgatctcct ggaacgcttc
      541 gccgccgctc cgccgcatgg cgtggcgctc ggcggcacgt tcaacgccca tccccgctcg
      601 gtggcggcgg ccctcgcgac catcgagcga atggcggaac cggacgccta ccctgccctc
      661 tacgccaagg gcgaggcgct ggcggcagca ctggagcggg cggtcgccgc ttccggccta
      721 gatgccgtcg tcacccgctt cggctcggtc gtggccctcc atttccaggc ggaggcaccg
      781 cgccgctacg aggatctggt gcatggcgac cttgctcgcg atgccgcctt tcgccggggc
      841 ctgctcgacc acggcatcgc cacggccgcc cagccgcttc ggcgcttctg cgtgagcctg
      901 gcgcacgagg accaccatat ggcccgcatc gaggaggccg cccacgcggt gctgaaggac
      961 cttgcccgcc agggcgccgg ggcagcgcca gcatgaccgt tccggcggcc cggctcctgt
     1021 cgctcgacgg gcgggtcgcc cttgtcaccg gcgcctcgcg cggcatcggc ctcgccattg
     1081 ccgaagcact ggcctcggcc ggggcgcatg tcgtcctgaa cgcccgcaac gccggccggc
     1141 tcgacgccgc cgtcgccgcc atccggacgt cgagcggcag cgccgaggcg gccgcgttcg
     1201 acgtgcgcga cgctggtgct gtgacggacg ccgtcgccaa catcggcgac cgcctcggcc
     1261 ggctcgacat cctggtgagc aatgtcggcg gtggggtgcg caagcccctg gccgagcaga
     1321 cggacgtcga tttcaccgcc gccatcgaca ccaatctcgt cgcctgcttc cgtctcgccc
     1381 gcgaatcgtc gcggttgatg gtgccacagg gcttcggccg cattctcgcc acctcgtcga
     1441 tcaactcctt cgtcgcccgg ccgaccatgg cgggctacgc ggcggccaag gccgggctga
     1501 acgggctggt gcgcgccctc gctgtcgagc tggcgccggc cggcatctgc gtcaacgcgc
     1561 tggccccggg ctactttccg accgaggcga acaggacctt gcgcgcgacc aaccccgatc
     1621 tcgtggccca ggtcgaggcc cgcaccccga tgcgccgctg gggcgccgtc gaggagcttg
     1681 gtgccgccgc catcttcctc tgctcgcctg gtgcctcgta ctgcaccggc caggtcctcg
     1741 ccgtcgacgg cgggcttctg gcatcactct agcggaagac acccatgcgc atcgccgttt
     1801 tgaacatcgg ccaggaatcc aacgacttca acccgctgcc gacgacgctc caggactacc
     1861 gctccttcgg cctctacgag ggccaggaga tgctggagaa gctgcgcggc ctcggcgagg
     1921 tcggcggcta tctcgaggcg atcgaggaat ccggcctcga ggtcgagacg atcccgatca
     1981 ttcgcggctg ggcctcggcg gcgggccggc tcgacgacgc gacgcggctc ttcttcgagg
     2041 agaagatcag aagcgggctc gaggccgccg gccggatcga cggactcgcc atccagctgc
     2101 acggcgcctg cgccgccgat ggcatggacg atgtcgaagg ggcgcagctc gcactctgcc
     2161 gcgagatcct gggacccgac gtgccgatcg tcctctcgct cgatcaccat gccaacgtca
     2221 cccggcagat ggtcgagaac gccgatgcgg tggtcggcca ccgaacccag ccgcatcagc
     2281 ccttcgagac cggcaagatc gcggccggga tactcctgcg catgctgcag ggcgaggtcc
     2341 gcccgaccgt cgccatgcgc aagctccggc tcctgtcgca ccaggagcag ttcctgacct
     2401 cgaagccgcc gatgaagacc tggttcgatc gcgcccgcga gatggagcag gatccgcgcg
     2461 tgctgcaggc ctcgcccttc ccgatgcagc cctggctcga cgtggccgag ggcggctggg
     2521 cggtggtggt gaccaccgat ggcgacaggg agctggccgg acggctcgcg gacgagctcg
     2581 ccgatctcgc ctggtcgatg cgcgccgagt tccagaagaa ggacgcggtc ggcgtcgacg
     2641 aggccgtgcg ccgggccgac gccatggacg ggctcgtgat cctcagcgac accggcgaca
     2701 ccgtcttcgg cggggccgcc ggcgacagca acctgatcct cgaatcgatt ctccgccagg
     2761 gcatcgaggg ccgtgccctg gtcccgatga tcgcacccga ggccgtggcg aagctcgcca
     2821 aggccggcgt cggcgccgag gtcacgctgc cgctcggcgg ccatgccact ggcttcttcg
     2881 agcccttgac cgtcaccggc acggtgcgcg ccgtgggcgg cggtgtcatc ccggtggcga
     2941 agcacaacca gctcgaggtc gacctggggc aggcggtcat cttcgaggtc ggcccggtga
     3001 ccatgctgct gaccgagctt cgcgcggtcg ccggcaacct gcccgagccc tatcgcgccc
     3061 tcggcgtcca gcccgaggac taccagatgg tggtgctcaa gaccgcgtcc aacttccagt
     3121 atttccagcc gatcgcgtcc ggcgtgatcc gggtcgacac gaaggggccg ggccagtcgg
     3181 acatcgccac cctgccctgg cagcgccagc cccggccggt ctacccgctc gacgagatcg
     3241 actcctggcg cggctgacgg gcctcttcgc cctgccattg cttgtgcgcc tttcgcaact
     3301 cacgcaaact gcccgcaaca ggccctggcg aggcagggcc gaacagatca ggaggtgagc
     3361 gccagatgag cattcaacgt cgccggctgc tttcgaccac cgtcgccctt tccctgggcg
     3421 tcttcatggc ttcggggatc aacgccaccg ccgaggcgca ggatggcggc acgctccgcg
     3481 tcgcgatcct ccaggacgtc agcaatttcg atccgcagtc gttcctctcg ctgaacttcc
     3541 ccctgatcaa gaacctctac gacagcctgc tcgaatacgg ccctgacggc gaggtcttgc
     3601 cgagcctggc gagcgcctgg acgatcgccg acgacaacat gtcggtgtcg ctgacgctgc
     3661 gcgacgacgt caccttccac agtggggcac ccatggacgc cgaggcggtg gcggcgacgc
     3721 tggccaaggc ggccgatcct gaaaggggcc agaacgtcta cgccaccatg gcaccagtcg
     3781 agaactggac ggtggacggc ccgcatcagc tcacggtgaa cttcaagggg ccgatccccg
     3841 accggcagag caccgatctc ctgcagttca tctcggtgat cgatcccgcc ggcatcgaga
     3901 cggtcgccac cgtgcccgcc ggcaccggcg ccttcacgct cgtcgagcgg gtgctcggcc
     3961 agagcgtgca gatggccgcc aacccgaact actggcggga cggcgagccc aaggtggacg
     4021 agctgctcat caccgtcttc ggcgacaacg acgccgcctc cgcggccctc gaatcggatg
     4081 ccgtcgacat gatctacggc gccggcgggc gggccgccgt gcggctgcgc gacagcggct
     4141 accagctcgt ccagggcccg gggccgctcg tgcaggtctt ccgcatcaac acgacccgtc
     4201 cgcccttcac caacgagaag ttccgtcagg ccttcaagta cctggtcgat cgcgaggcca
     4261 tcctcagggt cggctatgcc ggcctcggcg tggtcaccgc cctgccatgg gcgccggcca
     4321 gcccggcggc ggacccgtcc tacaacgaga cctatgcctt cgatctcgac aaggcgaagg
     4381 agctgctcga cgcttccggc ctcacgccgg ccgagatgag cgactggcag ctgctcgtga
     4441 acggcgccga tcaggacgcg gtgaccatca gccagctcgt ccagggcacc ctggccgagc
     4501 tcggcatcaa cgtggagctg gatctgaggc agggtgcgga gttcaccgac gccatgctcg
     4561 gcggcgacta cacggcgatc ttcgccggca tcggcaacat ccagaagttc ccgagccgga
     4621 tcgccaccaa ctccatctat cggaccagca acaacccgat cctcggcgag ccgcacccgc
     4681 accccgacta cgtcgcggcg atcgagcggg tgaacacgac gctcggctcc gacgaggagg
     4741 tccaggcggc ctacgaccat ctgaacgagg tcctgatcga ggcggccttc ggcgtcccga
     4801 cgaacaccta tgatgtcggg ctcatcgtcg ccgctcccga tgtcagcggc ttcaccccgg
     4861 acatcgacaa catgctcgtg gcgcgcacga tcaccgtggc gcggtgacgc gcgggctcga
     4921 ccgggcagga ggaggcggcc cggcctcggc tctccatggc tgagcccctc ctctccatcc
     4981 gcggcctcga ggtcaccttc aggggcaccg gccggccggt gcccgcggtg cgcggcatcg
     5041 atctcgatgt cgccgccgac gaggtgctcg gcatcgtcgg cgaatcgggc tccggcaaga
     5101 gtgtcacctc gctggcgatg actggcctcc tgccctcgac ggccgtggtc gggggctcga
     5161 tcaggatcgg cggcgtcgag gtgaccggtg cggacccgga ggtgctgcgc cagatgcgcg
     5221 gccgcgatgt cggcatgatc ttccaggacc cgaccaccag cctgaacccc gtcctgccca
     5281 tcggccgcca ggtcaccgaa ggccaggtcg cgcacggcct cctgcgcccg gccgaggcat
     5341 ccggccgggc agtcgagctg ctggcggagg tcgacatccc cgaccccgcc ggccgcgccc
     5401 tgcagttccc gcaccagttc tcgggcggca tgcgccagcg ggcggtgatc gccatggcga
     5461 tggcaggccg gccccggctg atcatcgccg acgagccgac caccgccctc gacgtgaccg
     5521 tccaggccca ggtcctgacc ctcctgcgcc gacggcaggc cgccaccggc gctgccgtga
     5581 tcctgatcac ccacgatctc ggcgtcatcg ccgagacggc gcaccgggtc gtggtgatgt
     5641 acgccggaaa gatcgtcgag gccgcaccgg tcgccgagat cttcgcgcgg ccgcgccatc
     5701 cctacaccgt gggcctcttg aagagcctgc cgcgcctcga tcaggatacc cgtcgcctgg
     5761 tgtcgatcca gggccagccg ccgacgcccg gcgccatccc ctccggctgc gccttccatc
     5821 cgcgctgccc gatcggtgcc gcgcgcgagc gctgccggag cgaggagccg cgcttgcgcg
     5881 cggccggccc cgagggcatc gccgcctgcc acttcaccga cgagatcgag ggcctcctcg
     5941 cgccgccccc cgaggcggga ggcagtgcct cggccccgcg cgccgcgccg gccgagccgc
     6001 tgctcgtcgt cgacagcttg aaggtgcatt ttccgatccg ccgcgggctg ctccgccggc
     6061 agaccggcct cgtgcgcgcc gtcgacggcg tcagcctcga ggtccgcgcc ggcgagacgc
     6121 tcggcctcgt cggcgaatcc ggctgcggca agaccacgac cggccgggcg atcatggggc
     6181 tcatcgaggc gagcggcggc agcatcgact tcgccggcca ggacatcacg cagctcgacg
     6241 cggccgggct ccgagccgtg cgccggcacg tccagtacgt cttccaggac ccctacgcct
     6301 cgctgaaccc gatccgcacg gtcgaggcga tcgtggccga gcccctgcac atccacggcg
     6361 tcttcgatgc catgggtggg aatgcccgcg tcggctggct cctcgatctc gtcggcctct
     6421 cccgcaccat gacgggccgc tacccgcgcg agctctcggg cgggcagcgg cagcgggtcg
     6481 gcatcgcccg cgccctcgcc ctcgagcccc ggctcctgat cctggacgag ccggtggccg
     6541 ccctcgacgt ctcgatccag gcgcaggtgg tcaacctgct catggacctt caggtcgagc
     6601 tcggcctcgc ctatctcttc atcgcccacg atctctcggt ggtgcggcac atctcggacc
     6661 gggtcgccgt catgtatctc gggcggatcg tcgaggaggg caccagggac cagctctatg
     6721 cccggcccgt gcatccctat acccagtccc tgctctcggc cgtgcccgtg ccggagcccg
     6781 ggaacgccgg ccgccgcatc gtgctcgagg gcgacatccc caacccggcc gacccgccgg
     6841 gcggctgcgc cttccacccg cgctgctacc gggcgacaga acgctgcacg agggaagcgc
     6901 cggccttcgc cgcctatccg ggcctcgaca cccgggtcgc ctgccatcat gcggggccgc
     6961 tcaccgcgga cgagctgccg gccgccgccg aggtgctgac atgagcgcca gccagcgccc
     7021 ggccctggtc gccgcccgcc gcgtgctggc ccagaagagt gccttcatgg ccgccgtctt
     7081 cctcctcggc atggcgctca tcgcctttct cgccccgtgg atcctgcccg gcgacccgct
     7141 gacgcagaac ctccggcacg cgctcgagcc gccctcggcg gccttctggt tcggcgccga
     7201 cgagctcggc cgggatatcc tgacccgggt cgtctacggg gcgcggacct cgctggtcac
     7261 ggcgctcggt gcggtcgcca tcgccgggct gatcggggtg ccgatcggcc tcgtcgccgg
     7321 cttctatggc ggctggcgcg acgcggtgct gatgcgcctc gtcgacgtca tcctggcgct
     7381 gcccggcatc ctcttcgcca tggcgctgat cgccgtgatc ggccggagcc agatggcagc
     7441 gctcgtcgcg gtcggcatca ccggcattcc cagcttcgcc cggatcaccc gtgcccaggt
     7501 gctctcgctc cgccgccgcg atttcgtcat agccgtcgac gccatgggcg gctcggcgtc
     7561 gcacctcatg ttccgcacca tcctgcccaa ttcctggagc ccgatcctcg tccagatcgt
     7621 gatcctcgcg gccgtggcca tcctgctcga ggcggcgctc gcctttctcg gcgtcggcat
     7681 cccgccgcca acgccgagct ggggcgagat gctgcgcacc ggcaagtcct acctctacga
     7741 ggcgccctac tacgcc
//
LOCUS       DAOTFI010000226         7677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11439_length_7677_cov_7.391892, whole genome shotgun sequence.
ACCESSION   DAOTFI010000226 DAOTFI010000000
VERSION     DAOTFI010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11439_length_7677_cov_7.391892"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..596
                     /locus_tag="P5558_19090"
     CDS             123..596
                     /locus_tag="P5558_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRY26481.1"
                     /translation="MVDIQEVPLDALDRRLIEEYQRDLPLCPEPYAAMGEALGVSEEV
                     VLDRLGRLQERGVLSRVGAVIAPHRTGWSTLAAMAVPQERLDEVAELVNACPEVNHNY
                     AREHRLNLWFVVTAASRERVADVLAGIAGRSGLEVLDLPLERAYGIDLGFKPSWS"
     gene            587..1075
                     /locus_tag="P5558_19095"
     CDS             587..1075
                     /locus_tag="P5558_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRY26482.1"
                     /translation="MELSPADRRLLDALEGGLPLVPRPYAVLGERAGMAEAEVIRRLA
                     ALQAAGVVKRLGLVVRHHEIGYRANAMTVMDVPDDMVDAVGERLTRAADVTLCYRRPR
                     RLPGWPYNLFAMVHGRERGAVLARIDQLLDEHGLAGLPHAVLFSTRRFKQRGARYRAA
                     AA"
     gene            1095..1538
                     /locus_tag="P5558_19100"
     CDS             1095..1538
                     /locus_tag="P5558_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRY26483.1"
                     /translation="MDATDRRIVAGLQGGFPLCPRPYERAARELGLDEGELIRRLARL
                     VETGVLSRFGPLFNVEAMGGTYVLAAMAVPEDAFDRVAEMVNAHVEVAHNYEREHLLN
                     MWFVVAAETPERAEAVLGEIRAETGIEVLSMPKLEEYFVGLRLQP"
     gene            1535..2035
                     /locus_tag="P5558_19105"
     CDS             1535..2035
                     /locus_tag="P5558_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRY26484.1"
                     /translation="MRSGREPGDAATLERRLVQAIGTGLPLVPDPYAEVAAALGTDEA
                     TVIATLEAMLARGAIRRIGAVPNHYALGYRANGMAVFDVDDAMVAELGPLIGAFDFVS
                     HCYRRPRHPPLWPYNLFAMVHAKTRPAVETRVRQIRSRLGSACRGDTILYSRRILKKT
                     GLRLAR"
     gene            2062..3246
                     /gene="nirJ"
                     /locus_tag="P5558_19110"
     CDS             2062..3246
                     /gene="nirJ"
                     /locus_tag="P5558_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HRY26485.1"
                     /translation="MFRITNFMREVVWPSRIGPARKPPGPVVIWNLTRRCNLRCRFCY
                     SGSADVAYADELSTDEVVTVMQDLRAFRVPALILSGGEPLLRPDIFELSAEAKRLGFY
                     VALSTNGTRIDETNIERIAELDYDYVGISLDGTEPTHDHLRGEPGAFAAAMRGVERCQ
                     AIGIKVGLRFTLTRDNAAELSTILDLIDRRGIDKFYLSHLNYGGRGFKVRGEDAQDVM
                     TRDALELIFETCLRHINEGRNTEFVTGNNDADGVFLLHWVRKNFPARAAHIQALLERW
                     GGNASGVNVANIDNRGFVHPDTYWWHHKLGNVRDRPFSEIWADTSADPVFAGLKQRPR
                     PVTGRCGACRHLAICNGNTRVRAARTTGDPWAADPGCYLTDAEIGLPGETAGREAAHA
                     AE"
     gene            3233..4771
                     /locus_tag="P5558_19115"
     CDS             3233..4771
                     /locus_tag="P5558_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRY26486.1"
                     /translation="MPRSRPALAALVLCWAGMAEAAPDGAALYAENCAECHGEARLGG
                     TGPALLPDNLGRLKPAQAAEVIRGGRAQTQMPAFEGVLDEATIEALVALVYTPPETVP
                     EWGMAEIRASHETTGAVPAEAPIYDADPLNLFVVVETGDHHITILDGDRFEPLRRLPT
                     AFALHGGPKFSPDGRFVYLASRDGWIEKIDMWGLEKLAKVRAGINTRNLALSHDGRFV
                     LVGNYLPNSLTVLDAEDLRPLAVIPVADIRGTGSRVSAVYAAPPRQSFIVALKDVAEI
                     WEIPIGPRAVKVSGLAHSNESGAEEVVDSADPFPIRRILLDEPVDDFFFTPDYRVLLG
                     SARSGDRVVAIHLDVGREIAELPLPGMPHLGSAAAFEWQGRQVMATPHLRDAALSVID
                     IERFEVVDEIETLGPGFFLRAHPATPYAWVDVFFGPDADAVQVLDTRTLELVRTIRPS
                     PGTTAGHVEFTRDGSHALVSVWADEGAVVVYNAHTLEEVKRIPMRRPVGKYNVHNRLA
                     AAGD"
     gene            4737..5471
                     /locus_tag="P5558_19120"
     CDS             4737..5471
                     /locus_tag="P5558_19120"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRY26487.1"
                     /translation="MSTIASPLPATERVPAPGPALLLCAHGARGVAGAAARHAGVLRR
                     QGRFATVEACALYGAPKLETVLARLAPGPLTVLPFMMAEGYTLQVLEERLRAARPDAR
                     LAAALGAHAGIADLLVARALKGLRAHGREPGETALLLVGHGTTRHPDSARTAKRHAAA
                     IRARGIFAEVGVAFLDEAPAVAETVASLAAPFIAGVGFLTDAGQHGASDVARLMAETG
                     RAGVYFGPIGPDPALLPLIVEAATLQ"
     gene            complement(5465..6496)
                     /locus_tag="P5558_19125"
     CDS             complement(5465..6496)
                     /locus_tag="P5558_19125"
                     /inference="COORDINATES: protein motif:HMM:NF006564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HRY26488.1"
                     /translation="MKQGVATHGQAAAPALAAEHPFAAVVRTVAKGEKLGRSLTMEEA
                     REAMRLVLEGGVEPVQLGAFLAVLRYRKETGAELAGFALAARDRLTCGTLPATALDWP
                     SYADRHRQLPYFVLAALLAAGAGVRVLMHGVAGEGPAGTRAVLQALGVPLATDAGEAE
                     RHMAERSFAYLPLEAICPDLERLFALRPLLGLRSPANTYARELNPAAAPCQIQGVFHP
                     NYADLHAQAASRLGQPRAAIFKGGGGEGQINPLKPVALTLVENGQVRRETWPALLEAD
                     DAGWRDEPLEPARVVELWRGRCRAPGPEAAILGTAAVALHLTGRSPTPESALAMAREL
                     WESRDRARY"
     gene            complement(6573..7016)
                     /locus_tag="P5558_19130"
     CDS             complement(6573..7016)
                     /locus_tag="P5558_19130"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRY26489.1"
                     /translation="MPLLPWSERFKLGIPSVDHEHKELVDLINELYDSLGHAPTKDAV
                     MAFLEEINARIHAHFALEEKVMRGMRYDELDAHKHEHEDLLDGIHDIMDRTEAGGFVD
                     LEAELGPRLQAWFGNHFATKDARLHHFMEEQGIDEESFARQHPDA"
     gene            complement(7034..7672)
                     /locus_tag="P5558_19135"
     CDS             complement(7034..7672)
                     /locus_tag="P5558_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRY26490.1"
                     /translation="MIVAAENAVRRGLLLPLLAIAFMGVTGWLASSTLPWRDLVAEGP
                     AAPVIETRSLLALVDHRGQRLAPAALEKPATLVFFGYTHCPDVCPLELATMAAALDRL
                     GPAADDVAAFFVSVDPERDTPEQLAAYVALFHPRITGLTGTTQAVAAAAGAFRAYYAR
                     VEQADGAYTMDHFARTLVLDRDGQLRAAVPFDSPPETLESAIRPLLAGVPAS"
BASE COUNT         1072 a         2714 c         2774 g         1117 t
ORIGIN      
        1 gacgccaacc gcatcgatat cgtcgacacc gagaccttcg agacggtggg gtcgctcgag
       61 gccgaggcgc cgagcggcat cttcttcacc gcccgcgccc atcggctggg gtattgaggg
      121 ccatggtcga catccaggag gttccgctcg acgccctcga tcgccggctg atcgaggagt
      181 accagcgcga cctgccgctc tgcccggagc cctacgcggc catgggcgag gcgctcgggg
      241 tgagcgagga ggtggtgctc gaccgccttg gccggctcca ggaacggggc gtgctcagcc
      301 gggttggtgc tgtgatcgca ccgcatcgca ccggctggag cacgctcgcc gccatggcgg
      361 tgccgcagga gcgcctcgac gaggtggccg agctggtcaa tgcctgcccc gaggtcaacc
      421 acaactacgc ccgcgagcac cggctcaatc tctggttcgt cgtgaccgcg gccagccggg
      481 aacgggtggc cgacgtgctc gccggcatcg ccggacgcag cgggctggag gtgctcgacc
      541 tgccgctgga gcgcgcctac ggcatcgatc tcgggttcaa gccgtcatgg agctgagccc
      601 ggccgatcgg cgcctgctcg acgccctcga gggcgggctg ccgctcgttc cacgccctta
      661 tgcagtgctg ggcgagcgtg ccgggatggc cgaggcggag gtgatccggc ggctcgccgc
      721 actccaggcg gcaggcgtgg tgaagcggct cggcctcgtg gtccggcatc acgagatcgg
      781 ctaccgggcc aacgcgatga ccgtgatgga tgtgccggac gacatggtcg acgcggtcgg
      841 cgagcggctg acgcgggctg ccgacgtgac gctctgctat cgccggccgc gccgcctgcc
      901 gggctggccc tacaacctct tcgccatggt gcacggccgc gagcgcggcg ccgtgctcgc
      961 ccgcatcgac cagctcctcg acgagcacgg tctcgccggc ctgccgcatg cggtgctctt
     1021 cagcacccgg cgcttcaagc agcgcggtgc gcgctaccgg gcggcggcgg cctgatgccc
     1081 cggagggtcg cctgatggac gccaccgacc gccgcatcgt ggccgggctg cagggcggct
     1141 tccccctgtg cccgcggccg tacgaaaggg cggcccggga gctcgggctc gacgagggcg
     1201 agctgatccg tcgcctggca cggctggtcg aaaccggcgt gctcagccga ttcggcccgc
     1261 tcttcaatgt cgaggccatg ggcggcacct acgtcctggc ggccatggcg gtgcccgagg
     1321 atgcgttcga ccgggtggcg gagatggtca atgcccatgt cgaggtcgcc cacaactacg
     1381 agcgcgagca tctcttgaac atgtggttcg tggtggcggc cgagacgccc gagcgggcgg
     1441 aggcggtgct cggcgagatc agggcggaga ccggcatcga ggtgctgtcg atgccgaagc
     1501 tcgaggagta cttcgtcggt ctgaggctgc agccatgagg tcggggcggg agccgggcga
     1561 cgctgcgacg ctcgagcggc ggctggtgca ggcgatcggg accggcctgc cgctcgtccc
     1621 cgacccctat gccgaggtcg cggcggcgct cggcaccgac gaggcgacgg tcatcgccac
     1681 cctcgaggcc atgctcgccc gaggcgccat ccgccgcatc ggcgccgtgc cgaaccacta
     1741 cgcgctcggc taccgggcca acggcatggc ggtcttcgac gtcgacgacg cgatggtggc
     1801 cgagctcggg ccgctgatcg gcgccttcga tttcgtcagc cactgctacc gccggcctcg
     1861 ccatccgccg ctctggccct acaatctctt cgccatggtc cacgccaaga cgcgcccggc
     1921 ggtcgagacc agggtccggc agatcaggag ccggctgggc tccgcgtgcc gcggcgacac
     1981 gatcctctac agccgccgca tcctcaagaa gaccgggctt cggctcgccc ggtagccgcc
     2041 tggcagaaac gggagacgtc catgttccgg atcaccaact tcatgcgcga ggtcgtctgg
     2101 cccagccgca tcgggccggc gcgcaagccg cccggaccgg tggtcatctg gaacctgacg
     2161 cgccgctgca acctgcgctg ccgcttctgc tactcgggct cggccgatgt cgcctatgcc
     2221 gacgagctct cgaccgacga ggtcgtgacg gtcatgcagg acctgcgcgc tttccgcgtg
     2281 ccggcgctca tcctctccgg cggcgagccc ctgcttcgcc ccgacatctt cgagctctcg
     2341 gccgaggcca agcgcctggg cttctacgtc gccctctcga ccaacggcac gcggatcgac
     2401 gagaccaaca tcgagcggat cgccgagctg gactacgact atgtcggcat cagcctggac
     2461 ggcaccgagc cgacccacga ccacctcagg ggcgagccgg gtgccttcgc cgccgccatg
     2521 cgcggcgtcg agcgctgcca ggcgatcggc atcaaggtgg ggctgcgctt cacgctgacg
     2581 cgggacaacg cggccgagct ctcgaccatc ctcgatctca tcgaccggcg cggcatcgac
     2641 aagttctacc tctcgcacct caactatggc ggccgcggct tcaaggtcag gggcgaggac
     2701 gcccaggacg tcatgacccg cgatgccctg gagctgatct tcgagacctg tctgcggcac
     2761 atcaacgagg gccgcaacac cgagttcgtc accggcaaca acgacgccga cggcgtcttc
     2821 ctgctgcact gggtgcgcaa gaacttcccc gcccgggccg cgcacatcca ggccctgctc
     2881 gagcgttggg gcggcaatgc gtcgggcgtg aacgtcgcca atatcgacaa tcgcggcttc
     2941 gtccacccgg acacctactg gtggcaccac aagctcggca atgtccggga ccgcccgttc
     3001 agcgagatct gggccgacac gtcggccgac cctgtcttcg ccgggctgaa gcagcgccca
     3061 cgcccggtca ccgggcgctg cggcgcctgc cgccacctcg cgatctgcaa cggcaacacg
     3121 cgggtccggg cggcccgcac gacgggcgat ccctgggccg ccgaccccgg ctgctacctc
     3181 accgacgccg agatcgggct cccgggcgag acggccgggc gcgaggccgc gcatgccgcg
     3241 gagtaggccg gctctcgccg ccctcgtgct gtgttgggcc ggcatggcgg aggcggcacc
     3301 ggacggtgcg gcgctctatg ccgagaattg cgcggaatgc catggtgagg cacggctcgg
     3361 cggcaccggg ccggcgctgc tgccggacaa tctcggccgg ctgaagccgg cccaggcggc
     3421 ggaggtcatc cggggcggcc gggcgcagac gcagatgccg gccttcgaag gcgtgctgga
     3481 cgaggccacg atcgaggcgc tcgtcgcgct cgtctacacc ccgcccgaga ccgtgccgga
     3541 atggggcatg gcggagatcc gggcgtcgca cgagacgacc ggtgcggtgc cggccgaggc
     3601 gccgatctac gacgccgatc cgctcaatct cttcgtcgtg gtcgagaccg gcgaccacca
     3661 catcaccatc ctcgacggcg accgcttcga gccgctgcgc cgcctgccca ccgccttcgc
     3721 gctgcatggc ggccccaagt tcagcccgga tggccggttc gtctatctgg cctcccgcga
     3781 cggctggatc gagaagatcg acatgtgggg gctggagaag ctggcgaagg tcagggccgg
     3841 gatcaacacc cgcaatctcg ccctctcgca cgacggccgc ttcgtcctcg tcggcaacta
     3901 cctgcccaac agcctcaccg tgctcgatgc cgaggatctc cggccgctcg cggtcatccc
     3961 ggtggccgac atccgcggca ccggctccag ggtgagcgcc gtctacgccg ccccgccgcg
     4021 ccagagcttc atcgtggcgc tcaaggacgt ggccgagatc tgggagatcc cgatcggccc
     4081 ccgcgccgtc aaggtcagcg gcctcgccca cagcaacgag agcggggcgg aggaggtcgt
     4141 cgattccgcc gacccgttcc cgatccgccg catcctgctg gacgagccgg tcgacgattt
     4201 cttcttcacc cccgactacc gcgtgctcct cggctcggcc cgcagcggcg accgcgtggt
     4261 cgccatccat ctcgacgtcg gccgcgagat cgccgagctg ccgctgccgg gcatgccgca
     4321 tctgggctcg gccgctgcct tcgaatggca gggccgccag gtcatggcga cgccgcatct
     4381 gcgcgacgcg gcgctcagcg tcatcgacat cgagcgcttc gaggtggtgg acgagatcga
     4441 gacgctgggc cccggcttct tcctccgggc ccatcccgcc accccctatg cctgggtcga
     4501 cgtcttcttc ggccccgatg ccgatgccgt gcaggtgctc gacacgcgca ccctcgagct
     4561 ggtgaggacc atccgcccga gccccggcac gacggccggg catgtcgagt tcacgcggga
     4621 cggcagccac gctctggtca gcgtctgggc ggacgagggt gcggtcgtcg tgtacaatgc
     4681 gcacaccctc gaggaggtca agcgaatccc gatgcgccga ccggtaggga agtacaatgt
     4741 ccacaatcgc ctcgccgctg ccggcgactg agcgggtgcc cgcgcccggc cccgcgctgc
     4801 tgctctgcgc gcacggtgcc cgcggcgtgg ccggcgccgc ggcgcggcat gcgggcgtgc
     4861 tgcggcggca gggccgcttc gccacggtcg aggcctgcgc gctctatggt gccccgaagc
     4921 tggagaccgt gctcgcgcgg ctggcgccgg ggccgctgac cgtgctgccc ttcatgatgg
     4981 ccgagggcta cacgctgcag gtgctcgagg agcggctgcg ggcggcccgg cccgacgccc
     5041 ggctggctgc ggcccttggc gcccatgcgg ggatcgccga tctgctcgtg gcaagggcgc
     5101 tcaagggcct gcgcgcccac ggccgcgagc cgggcgagac ggcgctgctg ctggtcgggc
     5161 acggcaccac gcggcatccc gattcggccc ggacggcgaa gcgtcacgct gcggcgatca
     5221 gggcacgggg gatcttcgcc gaggtggggg tggcctttct cgacgaggcg ccggccgtgg
     5281 cggagaccgt cgcaagtctc gccgcgccct tcatcgccgg cgtcggcttc ctcaccgatg
     5341 ccggccagca cggggcgagc gacgtggcgc ggctcatggc cgagaccggt cgcgccggcg
     5401 tctatttcgg cccgatcggc cccgatcccg ccctcctgcc gctgatcgtc gaggcggcca
     5461 cgcttcagta gcgcgcgcgg tcgcggctct cccagagctc gcgggccatg gccagggcac
     5521 tttcgggcgt gggcgacctg ccggtgagat gcagggccac ggcggccgtg ccgaggatgg
     5581 ccgcctcggg gccgggggcg cggcaccgcc cgcgccagag ctcgacgacg cgggcgggct
     5641 cgagcggctc gtcgcgccag ccggcgtcgt ccgcctcgag aagggcgggc caggtctcgc
     5701 gccgcacctg tccgttctcg acgagcgtga gggcaacggg cttcagcggg ttgatctgac
     5761 cctcgccgcc accgcccttg aagatggcgg cgcgcggctg gccgaggcgg ctcgcggcct
     5821 gggcgtgcag gtcggcgtag ttggggtgga agacgccctg gatctggcag ggtgccgcgg
     5881 ccgggttgag ctcgcgcgca taggtgttgg ccggcgagcg caggccgagg agcgggcgca
     5941 gcgcgaagag ccgctcgagg tcggggcaga tggcttcgag cggcagatag gcgaaggagc
     6001 gctccgccat gtgccgctcc gcctcgccgg cgtccgtggc gagcggcacg ccgagcgcct
     6061 gcagcacggc gcgcgtgccg gccgggccct cgccggcgac gccgtgcatc aggacgcgca
     6121 caccggcgcc cgctgccagg agggccgcca ggacgaaata gggaagctgg cggtggcggt
     6181 cggcatagga aggccagtcg agggccgtgg ccgggagggt gccgcaggtc aggcggtcgc
     6241 gggcggccag cgcgaagccg gcgagctccg ccccggtctc cttgcgatag cgcagcacgg
     6301 cgaggaaggc gccgagctgc accggctcga ccccgccctc gaggacgagg cgcatcgcct
     6361 cccgggcctc ctccatggtc aggctgcggc cgagcttctc gcccttggcg acggtgcgga
     6421 cgacggcggc gaaggggtgc tcggccgcca gggccggcgc cgcggcctgt ccatgcgtgg
     6481 ccacgccttg cttcatgatc gcttgctccc tgatctactc ggccacgcgc cgcaatctgc
     6541 cacggccggc tgcttcccgg ccttgacgtc ggtcaagcgt cgggatgctg gcgggcaaag
     6601 ctctcctcgt cgatgccctg ctcctccatg aagtgatgca gccgggcgtc cttggtggcg
     6661 aaatgattgc cgaaccatgc ctggagcctg gggccgagct cggcctccag atcgacgaag
     6721 ccaccggcct cggtccggtc catgatgtcg tgaatgccgt ccagcaaatc ctcgtgctcg
     6781 tgcttgtggg catcgagctc gtcatagcgc atgccgcgca tgaccttctc ctcgagcgcg
     6841 aaatgcgcgt ggatccgggc gttgatctcc tcgagaaagg ccatgacagc gtccttcgtc
     6901 ggcgcgtggc cgaggctgtc gtagagctcg ttgatgaggt cgaccagctc cttgtgctcg
     6961 tggtcgaccg aagggatgcc gagcttgaac ctctcggacc agggcaggag cggcatggtc
     7021 gcctccttgc tgttcagctc gccgggacac cggcgaggag tggtcggatg gcgctctcca
     7081 gggtctcggg cgggctgtcg aagggaacgg ctgcccgtag ctgcccgtcc cggtcgagga
     7141 ccagcgtgcg ggcgaaatgg tccatggtgt atgcaccgtc cgcctgctcg accctagcat
     7201 agtaggcacg aaaggcgcca gcggcggcgg cgacggcctg ggtcgtcccg gtgagcccgg
     7261 tgatccgcgg gtggaaaagc gcgacatagg cggcgagctg ctccggcgtg tcgcgctccg
     7321 ggtcgacgct gacgaagaag gcggcgacgt cgtccgcggc cgggccgagc cgatcgaggg
     7381 cggccgccat ggtggcgagc tcgagcgggc agacgtcggg gcagtgcgtg tagccgaaga
     7441 agacgagcgt ggcgggcttc tcgagcgccg ccggggcgag cctctgaccc cggtggtcga
     7501 cgagggcgag gaggcttcgc gtctcgatca ccggcgcggc cgggccctcg gccacgaggt
     7561 cgcgccaggg cagggtcgac gaggcgagcc agccggtgac gcccatgaag gcgatggcga
     7621 gaagcggcag gagcagcccg cgccggacgg cgttttccgc tgccactatc atttcga
//
LOCUS       DAOTFI010000227         7659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11468_length_7659_cov_7.431483, whole genome shotgun sequence.
ACCESSION   DAOTFI010000227 DAOTFI010000000
VERSION     DAOTFI010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11468_length_7659_cov_7.431483"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="P5558_19140"
     CDS             <1..785
                     /locus_tag="P5558_19140"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRY26491.1"
                     /translation="FGLQQGSQVVFSGVPVGRVTAIAIDRDDPSKVLVTVELAQTTPV
                     TTATTAMLVPQGVTGLVVVELRGGDPAAPPPPAPDGAPPRIAGLPSALEQVFTSTPLL
                     LARGVTVLERLGTALSDANIEALGETLDHVEALTRTLAERGDDLDRLLGIGAELGEEA
                     RATLAALAPLAALGPSGEVALAEVEAAAVAARNLAWRIDRLLAESEQPIIDFGDGGLY
                     EFGEMVREIRVLVAALSRIATDFERDPAGFIIGGSRRGFTPP"
     gene            782..1423
                     /locus_tag="P5558_19145"
     CDS             782..1423
                     /locus_tag="P5558_19145"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HRY26492.1"
                     /translation="MRQLDRRTLLAGALGLPLGACASLIPLPGQRAPTLYSLTPKTTF
                     NEELDELDVRILVEPPSAAGGLNTARIALKPDPTLLDYYADALWIEVVPIMVQTLVTE
                     SLDASGSLDVLGPDAVSLRPDYILRLHIREFQAEYDQGLEAPPLVNVRLQARLLGMPR
                     RQSLASFAAQQFARADGTGLDRIVLAYDEALGGVLRQIVQWAVAEIARAEGAM"
     gene            1500..2117
                     /locus_tag="P5558_19150"
     CDS             1500..2117
                     /locus_tag="P5558_19150"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY26493.1"
                     /translation="MDVKTLCLGVLTAQDLTGYDIKRTCEEVFRHFFVAGFGSIYPAL
                     AELQRDGLVSVTSVEQKKRPDKKVYRITDAGRRALEAELVATAPRHKVRSEFLALMYF
                     AHLMPPERVDAVLGEMIESFENKLVEDLAVFDGPSAPAELTPGQQFALGYGRTVLAAA
                     LAYCKRQRPQLARRLRGEADEPQPGDQPLQVANDPGPRPATLAGE"
     gene            2120..3268
                     /locus_tag="P5558_19155"
     CDS             2120..3268
                     /locus_tag="P5558_19155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY26494.1"
                     /translation="MRRATLLAVLLAVAIAGWLASPHLAPLLGLGGETPPAGSTAVVG
                     QAAAPAERLTQVRVRRSEAEPMARQQVLNGRTQANRSVRVAAEVPGEVIEIVGEKGDQ
                     VAEGDLLARLDPRDRAAALAEARATLEQRRIEAEAAERLGQRGFQAETQVAGARAALE
                     QARYQVRRSEVALEQTSITAPFAGVLEERPIELGSFLEVGDTVGTLVELDPLLVVTDV
                     PEAAILSIVPGSTAEVKFIDGTVREGRVRFVARQANAATRSFRVEIVLPNPDLRIPAG
                     ISTTVAINLAPVPAHAVSPGILVLDDRGVLGVKSVDDDGKVAFHPAEIVRAEGDVVWL
                     AGLPSTIDLITVGQGFVMPGQPVEAVPEATVAALVAGGEPGDTPGTPQ"
     gene            3265..6450
                     /locus_tag="P5558_19160"
     CDS             3265..6450
                     /locus_tag="P5558_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY26495.1"
                     /translation="MNGIIAATLARTRAVLLILALLLVAGVVAYTSIPKEAEPDIQIP
                     VVYVSVPYEGISAYDAERLLARPVETELQGLEGVKEIKSVSTQGRATITIEFDTGVDI
                     DQAVSDVRDKVDRAKAELPAGADEPSVQEINLALFPVLVVTLSGDLPERELLRIARDL
                     QDRIEGLRNVLEVEIAGEREELLEAIVEPLAIESYGLELEAVVRAVDRNNQLVAAGAL
                     DAGGGRFDIELPGVFETYEDLMRLPLKVDGEAVVRLGDVASVRRTFKDAVGYARVDGR
                     PALALEVSKRIGANIIDTIAEVRATVEEARASWPAGLEVGYAQDKSDNVRSMLTDLQN
                     NVLAAVLLVMIVVVAALGLRSALLVGFAVPGSFLTGILVLGLFGLTINMIVLFALILA
                     VGMLVDGAIVVTELADRKMSEGMAKRDAYRLAAQRMATPITSATLTTLAAFLPLLFWP
                     GIVGDFMKYLPITLLATLSASLLMALVFVPALGSIFGKATATDPATMKLLNASEGGDL
                     RELGGVTGWYVRVLDRLLRRPSLVALAASLIAVGAYWAYGTYGRGVEFFPSVEPERGQ
                     LMVHARGDHSVRERDALVAEVERRIEGVDGIRQTYARTALSFRGDGIDEDVIGILQLE
                     FEPWQTRRPAVEIFAEIEERVAGLAGIHVEEREEEAGPPVGKPIQVEIGSNLPDRLPP
                     VVTAIRDKVDSMTGLREVTDSRPIPGIEWQMRVDREQAGRLGTDIATIGAHVQMVSNG
                     VLLGTYRPDDADDEVDIRARFPVEYRNLGQLDRLVVNTAKGAVPVSSFVDLGAEPRTG
                     DLTRIDGKRIYTVSADVAPGVLADDKVQELRAWLAAQDFDPAVSVVFKGEDEEQRDAQ
                     AFLSKAFGVALFLIAIILVTQFNSFYQAALILSAVVFSTVGVLLGLLVTNQPFGIVMS
                     GIGVIALAGIVVNNNIVLIDTYNELRKRGLDVREAILRTGAQRLRPVMLTTITTILGL
                     MPMVLKANVDLFARTITLGGPSADWWAQLATAVAGGLAFATLLTLALTPCLLMLRQTT
                     AESWQALRRRLRNRRHRRRESPGGFQGAPAE"
     gene            6521..>7659
                     /locus_tag="P5558_19165"
     CDS             6521..>7659
                     /locus_tag="P5558_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY26496.1"
                     /translation="MDEFALTGIFLFVLFLLLGSGVWVALALLGVALVGMELFTSRPA
                     GDAMITTIWTGASSWTLTALPLFIWMGEILFRTRLSEDMFKGLAPWLANWPGRLLHTN
                     VVGCTVFAAVSGSSAATMTTVGKMSIPELRRRGYPEEMIIGTLAGSGTLGLMIPPSLI
                     LIVYGVTINESISRLFIAGIIPGLTVAALFMGYVVVWSWLHPGRIPGGEAHVPFMAKL
                     RSSGGLLPILGLILAVLGAIYAGLATATEAASFGVVGALLLALLQGSLTRESFTQSLM
                     GAVRTSCMIAFILMGAAFLSLSMGFTGLPRALAEWIAGLGLSPLMLIAALTLFYIVIG
                     CFLDGISAVVLTMAVVEPMVRTAGIDMIWFGIFIVVVVEMAQITPP"
BASE COUNT         1049 a         2962 c         2484 g         1164 t
ORIGIN      
        1 tcttcggcct gcagcagggc agccaggtgg tgttcagcgg cgtgccggtc ggccgggtca
       61 ccgccatcgc catcgaccgg gacgacccgt cgaaggtgct cgtcacggtc gagctcgccc
      121 agaccacgcc cgtgaccacg gcgacgaccg cgatgctggt gccgcagggt gtcaccggtc
      181 tcgtcgtggt cgagctgcgc ggcggcgacc ccgcggcccc gccgcccccc gcccccgacg
      241 gcgcaccacc gcgcatcgcc ggcctgccgt ctgccctcga gcaggtcttc acctccaccc
      301 cgctgctcct ggcgcgcggc gtcaccgtgc tggagcggct gggcaccgcc ctctccgacg
      361 cgaatatcga ggcgctcggc gagaccctgg accatgtcga ggcgctgacc agaaccctcg
      421 ccgagcgcgg cgacgacctc gaccggctgc tcggcatcgg ggccgagctc ggcgaggagg
      481 cgcgcgccac gctcgcggcc ctggcacctc tcgcggccct cggcccgagc ggcgaggtgg
      541 cgctggccga ggtcgaggcg gcggccgtcg ccgccaggaa cctggcctgg cggatcgacc
      601 ggctgctcgc cgaatcggag cagccgatca tcgatttcgg cgatggcggc ctctacgagt
      661 tcggcgagat ggtccgcgag atccgcgtcc tggtcgcggc gctcagccgc atcgccaccg
      721 atttcgagcg cgatcccgcc ggcttcatca tcggcggcag ccgccgcggc ttcaccccgc
      781 catgaggcag ctcgaccgcc gcaccctcct ggccggcgct ctcggcctgc cgctcggcgc
      841 ctgcgcctcg ctcatcccgc tgcccggcca gcgcgcaccg accctctaca gcctgacgcc
      901 caagacgacc ttcaacgagg agctggacga gctcgacgtc cgcatcctgg tcgagccgcc
      961 atccgccgcc ggcgggctga acacggcgcg gatcgcgctc aagccggacc cgaccctgct
     1021 cgactactat gccgatgcgc tgtggatcga ggtcgtgccg atcatggtcc agacgctggt
     1081 gaccgaatcg ctcgacgcga gcggcagcct cgacgtgctc ggccccgacg cggtgagcct
     1141 caggccggac tacatcctca ggctccacat ccgcgagttc caggccgagt acgaccaggg
     1201 gctggaagcg ccgcctttgg tcaacgtgcg cctgcaggcg cggctcctcg gcatgccgcg
     1261 ccggcaatcg ctcgccagct tcgccgcaca gcagttcgcc cgcgccgacg gcaccgggct
     1321 cgatcgcatc gtcctcgcct atgacgaggc gctcggcggc gtgctcaggc agatcgtgca
     1381 atgggccgtg gccgagatcg cccgggccga gggcgccatg tgacgacctg tccgacctga
     1441 ggggttgccc ggctatatcg atccaatata tatcggatcg agataacgat ggggccctca
     1501 tggacgtcaa gaccctctgc ctcggcgtgc tgaccgcgca ggacctgacc ggctacgaca
     1561 tcaagcggac ctgcgaggag gtgttccggc acttcttcgt cgccggcttc ggctcgatct
     1621 acccggcctt ggccgagctg cagcgcgacg ggctggtctc cgtcaccagc gtcgagcaga
     1681 agaagcggcc ggacaagaag gtctaccgca tcaccgatgc gggccgcagg gcgctggagg
     1741 ccgagctggt cgccaccgca ccgcgccaca aggtgcgctc ggagttcctc gccctcatgt
     1801 atttcgccca cctgatgccg cccgagcggg tcgacgcggt gctgggcgag atgatcgaaa
     1861 gcttcgagaa caagctggtc gaggacctcg ccgtcttcga cggcccctcg gcaccggccg
     1921 agctgacccc gggccagcaa ttcgcgctcg gctacggccg cacggtgctg gccgcggccc
     1981 tcgcctattg caagcggcag cggccgcagc tcgcccgtcg cctccggggc gaggcggacg
     2041 agccgcaacc cggggaccag ccgctgcagg tggccaacga cccggggccg cgacccgcca
     2101 ccctggccgg ggaatagcca tgcggcgcgc caccctcctc gccgtgctgc tggccgtggc
     2161 gatcgccggc tggctcgcct cgccccatct ggcgccgctc ctcggcctcg gcggcgagac
     2221 gccgccggcc gggtccaccg ccgtcgtcgg ccaggccgca gcaccggcgg agcgcctgac
     2281 ccaggtccgc gtgcgccggt ccgaggccga gcccatggcg cgccagcagg tgctcaacgg
     2341 ccggacccag gccaaccgca gcgtgcgcgt cgccgccgag gtgcccggcg aggtgatcga
     2401 gatcgtgggc gagaagggcg accaggtggc cgagggcgat ctcctcgccc gtctcgaccc
     2461 gcgcgaccgc gcggccgccc tcgccgaggc cagggccacg ctggagcagc ggcggatcga
     2521 ggccgaggca gccgagcgcc tgggccagcg cggctttcag gccgagaccc aggtcgccgg
     2581 ggcgagggcg gcgctcgagc aggcccgcta ccaggtccgc cggagcgagg tggcgctgga
     2641 gcagaccagc atcacggccc ccttcgccgg cgtgctcgag gagcggccga tcgagctcgg
     2701 cagcttcctc gaggtcggcg acacggtcgg caccctcgtc gagctcgacc cgctcctcgt
     2761 cgtcaccgac gtgcccgagg cggcgatctt gagcatcgtc cccggcagca ccgccgaggt
     2821 gaagttcatc gacggcaccg tgcgcgaggg ccgggtgcgc ttcgtcgcgc gccaggccaa
     2881 cgcggcgacg cgctccttcc gcgtcgagat cgtgctgccc aacccggact tgcggatccc
     2941 cgccggcatc agcaccaccg tcgcgatcaa cctcgccccg gttccggccc acgcggtctc
     3001 gcccggcatc ctcgtgctcg acgaccgggg cgtgctcggc gtcaagtcgg tcgacgacga
     3061 cggcaaggtc gccttccacc cggccgagat cgtccgggcc gaaggcgacg tcgtctggct
     3121 cgccggcctg ccctcgacca tcgacctgat caccgttggc cagggcttcg tcatgcccgg
     3181 ccagccggtc gaggccgtgc ccgaggccac cgtcgccgcc ctcgtcgccg ggggcgagcc
     3241 cggtgacact ccgggaaccc cgcaatgaac ggcatcatcg ccgccaccct cgcgcgcacc
     3301 cgggcggtcc tcctgatcct cgccttgctg ctggtcgccg gggtggtcgc ctacacctcc
     3361 atcccgaagg aggccgagcc ggacatccag atcccggtcg tctacgtctc ggtgccctac
     3421 gagggcatct ccgcctacga cgccgagcgg ctcctcgccc ggccggtcga gacggagctg
     3481 cagggcctcg agggggtcaa ggagatcaag tcggtctcga cccagggccg ggcgaccatc
     3541 accatcgagt tcgacaccgg ggtcgacatc gaccaggcgg tgagcgacgt ccgcgacaag
     3601 gtcgaccggg ccaaggccga gctgccggcc ggcgccgacg agccgtccgt gcaggagatc
     3661 aacctcgccc tcttcccggt gctcgtcgtc accctctcgg gcgacctgcc cgagcgcgag
     3721 ctcctgcgca tcgcccgcga cctccaggac cgcatcgagg gcctgcgcaa cgtcctcgag
     3781 gtcgagatcg cgggcgagcg cgaggaattg ctcgaggcga tcgtcgagcc gctggcgatc
     3841 gagagctacg gcctcgagct ggaagccgtg gtgcgcgccg tcgaccgcaa caaccagctc
     3901 gtcgcggccg gtgccctcga tgccggtggc ggccgcttcg acatcgagct gccgggcgtc
     3961 ttcgagacct atgaggacct gatgcgcctg ccgctcaagg tggacggcga ggcggtcgtg
     4021 cggctcggcg acgtcgcctc ggtgcgccgc accttcaagg atgccgtggg ctacgcccgg
     4081 gtcgacgggc gcccggccct ggcgctcgag gtcagcaagc gcatcggcgc caacatcatc
     4141 gacaccatcg ccgaggtccg ggccacggtc gaggaagcgc gcgcaagctg gccggccggg
     4201 ctcgaggtcg gctacgccca ggacaagtcg gacaatgtcc gctcgatgct gaccgacctg
     4261 cagaacaacg tcctggccgc cgtgctcctc gtcatgatcg tggtggtggc ggccctcggc
     4321 ctgcgctcgg ccttgctcgt cggcttcgcc gtccccggct cgttcctgac cggcatcctg
     4381 gtgctcggcc tcttcggcct caccatcaac atgatcgtcc tcttcgccct gatcctcgcc
     4441 gtcggcatgc tggtggacgg ggcgatcgtc gtcaccgagc tcgccgaccg caagatgtcc
     4501 gagggcatgg ccaagcgcga cgcctaccgg ctcgccgccc agcgcatggc gacgccgatc
     4561 acgtcggcca cgctcacgac gctcgccgcc ttcctgccgc tcctcttctg gccgggcatc
     4621 gtcggcgact tcatgaagta cctgccgatc acgctcctcg cgacgctctc cgcctcgctc
     4681 ctcatggcgc tggtcttcgt cccggccctg ggctcgatct tcggcaaggc gaccgccacc
     4741 gaccccgcca ccatgaagct gctcaacgcc agcgagggcg gcgacctgcg cgagctcggc
     4801 ggcgtcaccg gctggtatgt ccgcgtgctc gaccggctgc tccgccgccc ctccctcgtg
     4861 gcgctggcag caagcctgat cgccgtcggt gcctactggg cctacggcac ctacggccgc
     4921 ggcgtcgagt tcttcccgag cgtcgagccc gagcgcggcc agctcatggt gcatgcccgc
     4981 ggcgaccatt cggtccgcga gcgcgacgcc ctcgtcgccg aggtcgagcg ccgcatcgag
     5041 ggcgtcgacg gcatccgcca gacctatgcc cgcaccgccc tcagctttcg cggcgacggc
     5101 atcgacgagg acgtgatcgg catcctccag ctcgagttcg agccctggca gacccgtcgc
     5161 ccggccgtcg agatcttcgc cgagatcgag gagcgggtgg ccgggctcgc cggcatccac
     5221 gtcgaggagc gcgaggagga ggccggcccg cccgtcggca agcccatcca ggtcgagatc
     5281 ggctcgaacc tgcccgaccg cctgccgccc gtcgtcaccg ccatccgcga caaggtcgac
     5341 agcatgaccg gcctgcgcga ggtcaccgac agccggccga tccccggcat cgagtggcag
     5401 atgcgggtcg atcgcgagca ggccggccgc ctcggcaccg acatcgccac catcggcgcc
     5461 catgtccaga tggtcagcaa cggcgtgctg ctcggcacct accgccccga cgacgccgac
     5521 gacgaggtcg acatccgcgc ccgcttcccg gtcgaatacc gcaatctcgg ccagctcgac
     5581 cggctggtcg tcaacaccgc caagggtgcc gtgccggtct ccagcttcgt cgacctcggc
     5641 gccgagccgc gcaccggcga tctcacccgg atcgacggca agcgcatcta cacggtcagc
     5701 gccgacgtcg cccccggcgt gctcgccgac gacaaggtcc aggagctccg cgcctggctc
     5761 gccgcccagg atttcgatcc cgccgtctcg gtcgtcttca agggcgagga cgaggagcag
     5821 cgcgacgccc aggccttcct ctccaaggcc ttcggcgtcg ccctcttcct catcgccatc
     5881 atcctcgtca cccagttcaa cagcttctac caggcggcgc tgatcctctc cgccgtcgtc
     5941 ttctccaccg tcggcgtgct gctcggcctg ctcgtcacca accagccctt cggcatcgtc
     6001 atgtcgggga tcggcgtcat cgccctcgcc ggcatcgtcg tgaacaacaa catcgtcctg
     6061 atcgacacct acaacgagct gaggaagcgc ggcctcgatg tccgcgaggc catcctgcgc
     6121 accggtgccc agcgcctccg tccggtcatg ctcacgacga tcaccaccat cctcggcctc
     6181 atgccgatgg tgctgaaggc caatgtcgat ctcttcgccc gcaccatcac cctgggcggc
     6241 ccgtccgccg actggtgggc ccagctcgcc accgccgtcg ccggcggcct cgccttcgcc
     6301 accctcctca ccctcgccct gaccccctgc ctcctcatgc tccgccagac caccgccgaa
     6361 agctggcaag ccctccgccg ccgtctccgc aacaggcgcc atcggcgccg cgaatcgccc
     6421 ggcggcttcc agggagcacc ggcggagtag cctttgcggg cgggagcatt gccgctagcc
     6481 tgctccgcgg gccgagacac cagcggaaag ggccggcggc atggacgagt tcgcgctgac
     6541 cggcatcttt ctcttcgtgc tcttcctcct gctgggctcc ggcgtgtggg tggcgctggc
     6601 gctgctcggt gtcgccctcg tcggcatgga gctcttcacc tcgcgccccg ccggcgacgc
     6661 catgatcacg acgatctgga ccggcgcctc gtcctggacg ctgaccgccc tgccgctctt
     6721 catctggatg ggcgagatcc tcttccgcac ccggctctcc gaggacatgt tcaagggcct
     6781 cgccccctgg ctcgccaact ggccgggccg gctcctgcac accaatgtcg tcggctgcac
     6841 cgtcttcgcc gcggtctcgg gcagctcggc cgcgacgatg accacggtcg gcaagatgtc
     6901 gatcccggag ctccggcggc gcggctatcc cgaggagatg atcatcggca cgctggccgg
     6961 ctccggcacg ctcggcctga tgatcccgcc gtcgctcatc ctcatcgtct atggcgtgac
     7021 catcaacgaa tcgatcagcc gcctcttcat cgccggcatc atcccgggcc tcaccgtggc
     7081 cgccctcttc atgggctatg tcgtcgtctg gtcctggctc caccccggcc gcatcccggg
     7141 cggcgaggcc catgtcccgt tcatggcgaa gctccgctcg agcggcgggc tgctgccgat
     7201 cctcggcctg atcctcgccg tcctcggcgc catctatgcc gggctcgcca ccgccaccga
     7261 ggccgccagc ttcggcgtgg tcggggccct gctcctggcc ctcctgcagg gctcgctcac
     7321 ccgggagagc ttcacccaga gcctgatggg ggccgtccgc acctcctgca tgatcgcctt
     7381 catcctgatg ggtgcggcct tcctctcgct ctccatgggc ttcaccggcc tgccccgcgc
     7441 gctcgccgaa tggatcgccg gcctcgggct ctcgcccttg atgctgatcg cggcgctcac
     7501 cctcttctac atcgtcatcg gatgcttcct cgacggcatc agtgcggtcg tgctcaccat
     7561 ggccgtggtc gagcccatgg tgcggaccgc cggcatcgac atgatctggt tcggcatctt
     7621 catcgtcgtc gtcgtcgaga tggcgcagat caccccgcc
//
LOCUS       DAOTFI010000228         7621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11546_length_7621_cov_6.348401, whole genome shotgun sequence.
ACCESSION   DAOTFI010000228 DAOTFI010000000
VERSION     DAOTFI010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11546_length_7621_cov_6.348401"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="P5558_19170"
     CDS             complement(<1..354)
                     /locus_tag="P5558_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26497.1"
                     /translation="MATTPPWTMGMDNGALREPIDPGLLGTAELEPTGDFEAGSFQSF
                     TLTYTAGRYGIDDSGSLRVCFRFASDQSNPQFTDPKAPGYTTIEASNGAVLETRFDPK
                     GNIRPWDRTLWIKVVK"
     gene            complement(374..787)
                     /locus_tag="P5558_19175"
     CDS             complement(374..787)
                     /locus_tag="P5558_19175"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HRY26498.1"
                     /translation="MSGPRILRCHVGVGPVAEGEALVTDDNFSARYDLDRLQGIFSRP
                     QHKLHGQSYVGKVLVVNTAKGGVASAWMLHEMAARGMAPVAILFNRFNPILAQGAALA
                     DLAICDRFADGDITRLIETGEQVKVEPAVGLVTVG"
     gene            complement(784..2028)
                     /locus_tag="P5558_19180"
     CDS             complement(784..2028)
                     /locus_tag="P5558_19180"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HRY26499.1"
                     /translation="MRLTDIEEAMARGEHGAPRQWAIQHMQQVGRAFDAADLVEVHQA
                     HIMADTESLGEAGVAFLERLGQAPAADRRVRVPTITDPRGIDFAHYRKLGQTDAMANL
                     ERRAIDAFEALGVLMTDTCINYQTIMPPLPGEHQAYGDTGVVIYSNSVLGARSNFEGG
                     PSALAAALTGRTPRYGLHLDENRRGTHRFVVRTRPRDLAEWGALGALVGRAAGSYWAV
                     PVIEGIDGPPDSDALKHMGAAMASFGSIPLFHLVGITPEAPTLDAVCDPASLPVREID
                     AAELKSFFGEYGAPGEKVDVVVFAAPQLSLIEMQQVADLLDGRRIHEGTALLVATSPG
                     VAADADRMRLTERIEASGALVLRGMCFYQSYAREMAEANGWRRLLTNSAKLVNILGGY
                     GYAPSLMSMSDCVDAAVAGRTP"
     gene            complement(2066..3313)
                     /locus_tag="P5558_19185"
     CDS             complement(2066..3313)
                     /locus_tag="P5558_19185"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY26500.1"
                     /translation="MTRENAGFRYTSSFRRNLPAPAQTWSPPAKYNFAVGHNDRDEVP
                     TEALAEAAAAVIRRHGADLAVYNQGQNPLGFEGLRDFVAAKLNRLRGASCTADDILVT
                     SGSLQGIDLVNDLLLEPGDTVIFEAFTYSAMFSRAQRLGVHPVGAPLDDGGIEVEKLA
                     AVLEELKRKSIRPKYIYTIPTIQNPTGSILGLERRQQLLEVARAYEVPIFEDECYADI
                     IWRDGAPPALWALDPTQVIHIGSFSKSLAPALRVGYVAADRSVLARMLACKTDGGTAA
                     LDQMVIAEYFGTHFDSHLRELTDALEDKLRTLIEALDREFGTSVELTAPKGGLFVWLK
                     LPDHIDVRKLVEPARAAGVTFNPGPTVACDPEAAKHCMRLCYGLPTKAEIREGIAALA
                     RVFFEETGIPERGGNRPRRLVDT"
     gene            3437..4156
                     /locus_tag="P5558_19190"
     CDS             3437..4156
                     /locus_tag="P5558_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY26501.1"
                     /translation="MAEGTLEVRIWRGGDEGAFGAYAVPARENQTVLDVVTEVQRRFE
                     PSLAYRFSCRVGVCGSCAMTINGTPRWTCRTHVKRVAPEGRLTIEPLRNMKRVKDLAC
                     DMDGFLGKWASVGTFEGTATRADPPAAIDPASPARREVDAAIQCINCAVCHAACDVVA
                     WDPHYLGPAALNRAWTLYNDARVADRRAVLDGALQPGGCHTCHSHGACTEHCPVGLSP
                     TASIAGLKRAGLVSLLKGGRS"
     gene            4153..4491
                     /locus_tag="P5558_19195"
     CDS             4153..4491
                     /locus_tag="P5558_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HRY26502.1"
                     /translation="MSEARLFLLQRLTALVMAPLVLVHLVLIVVAVQGGLTAGEILGR
                     TQGSVFWGLFYGIFVLVAAIHAPIGLRNVLREWTGIGRRPIDWLMVAFGVLLLVLGWR
                     AVVAVVGGGA"
     gene            4488..4826
                     /locus_tag="P5558_19200"
     CDS             4488..4826
                     /locus_tag="P5558_19200"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HRY26503.1"
                     /translation="MRRGAGWWAAMGHRLSGLALAAFLPLHFLMLGRALGGAEALDSS
                     LKIAELPLVKVAEFGLVIALAVHLAFGLRVLAIELLPWPARDGLRLRLVYLGLAATVL
                     VGIAFAVSVV"
     gene            4826..6589
                     /locus_tag="P5558_19205"
     CDS             4826..6589
                     /locus_tag="P5558_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY26504.1"
                     /translation="MRIEESRCDILILGSGGAGLFAALHAAKADPALDIVVAVKGLLG
                     KSGCTRMVQGGYNVALAAEDSIERHFMDTIVGGKWLPRQDLAWRLCVGAVERVRELEN
                     EIGCFFDRNPDGSLHQKAFAGQSFDRTAHKGDLTGIEIINRLMEQVRSRAVRRLEEHR
                     AIELIPAADGSGLAGVLLIDIRMGEYRFVRAKAVLLATGAGPTMYLYHTPSGDKTCDG
                     LAMALRLGLALRDMEMVQFHPTGLLGGPHTRMTGTVLEEGLRGAGGHILNGGKERFLA
                     GYDPAGERATRDVVSRGIYAEMRAGRTTPMGGVYISMGHLGAEKVAGMFPGMVKRCAD
                     CGFDLAGGLVEVVPTAHYMMGGVEFEIDASTAVPGLFAAGEDCGGVHGANRLGGNGVA
                     NSTVYGGIAGDSMAAFVGREGVWRDPDNSVIEAGIARAERPFGQAPGDLAGLRDRLAQ
                     VMWDDVGVIRDALGIERGLAGIADCRAALLAQGVVDGDRRFNLTWHDWLNLESLLDLS
                     EVVGRAALARDDSRGAHFRADFPETGVLETSAYTRIRRDGDELRLDTAPVAFDIVEPG
                     QSLIEGAAGAPPAEAAIGSAA"
     gene            6586..7551
                     /locus_tag="P5558_19210"
     CDS             6586..7551
                     /locus_tag="P5558_19210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRY26505.1"
                     /translation="MIAHEQVETAAYEIMKKAAIDIPEDYLGGVKGMIDLETGDLSSF
                     VLKSMVDNWEAATEDRRPMCADTGLPRYYVKVGNEARVAGGFCGVEEALRSATARATH
                     DVPLRPNRVHPLWRTEHNNNVGINAPEIEWSFEPDVDWIDVTTVHKGGLFGTDYRMLF
                     PGDGIDGIKRVFLDTLIAFGKRGLACQPAIVGVGVGGSKDICMTLGKQAACLRVVGDA
                     NPDPRIAALEQELKELGNRIGMGAMGFVGSSMVVDCHVEVGYTHTGGMPVSVHTFCLS
                     SRRATARLHADGRTTYRTDPKWFTPYMRRETIGWPRSEPSSASAG"
BASE COUNT         1077 a         2471 c         2875 g         1198 t
ORIGIN      
        1 cttgaccacc ttgatccaca aggtgcggtc ccaggggcgg atattgccct tgggatcgaa
       61 gcgggtctcg agcacggccc cgttggaggc ctcgatcgtg gtgtagcccg gcgccttcgg
      121 gtcggtgaac tgcgggttgc tctggtccga ggcgaagcgg aagcagacgc gcagcgagcc
      181 cgaatcgtcg atgccgtagc ggccggccgt gtaggtcagg gtgaagctct ggaaggagcc
      241 cgcctcgaag tcgccggtcg gctcgagctc ggccgtgccg aggaggcccg ggtcgatcgg
      301 ctcgcggagc gcgccgttgt ccatgcccat ggtccagggc ggtgtcgtcg ccatcgtcgg
      361 cgctccttct ggctcagccg acggtgacga ggccgaccgc tggctccacc ttcacctgct
      421 cgcccgtctc gatgaggcgg gtgatgtcgc catccgcgaa gcggtcgcag atggccaggt
      481 cggcgagggc ggcaccctgc gccaggatcg ggttgaagcg gttgaagagg atggccaccg
      541 gcgccatgcc gcgggccgcc atctcgtgca gcatccaggc cgaggcgacg ccgcccttgg
      601 cggtgttcac caccagcacc ttgccgacat agctctgccc gtggagcttg tgctgcggcc
      661 gggagaagat gccctgcagg cggtcgaggt cgtagcgggc cgagaagttg tcgtcggtga
      721 cgagggcctc gccctcggcc accgggccga cgccgacatg gcagcggagg atgcgggggc
      781 cgctcatggc gtgcgccccg caacggcggc gtcgacgcag tcggacatgc tcatgagcga
      841 tggcgcatag ccgtagccgc ccaggatgtt gaccagcttc gccgagttgg tcagcaggcg
      901 gcgccagccg ttcgcctcgg ccatctcgcg ggcgtagctc tggtagaagc acatgccgcg
      961 cagcacgagc gcacccgagg cctcgatgcg ctcggtcagg cgcatgcggt cggcatcggc
     1021 ggcgacgccg ggcgaggtgg cgacgaggag tgctgtgccc tcgtggatgc ggcggccgtc
     1081 gaggaggtcg gccacctgct gcatctcgat gagggagagc tgcggggcgg cgaagacgac
     1141 gacgtcgacc ttctcgccgg gagcgccgta ctcgccgaag aagctcttca gctcggcggc
     1201 gtcgatctcc cggaccggca gcgaggccgg gtcgcagacg gcgtcgagcg tcggggcctc
     1261 gggggtgatg ccgacgaggt ggaagagcgg gatcgagccg aagctcgcca tcgccgcacc
     1321 catgtgcttg agcgcgtcgg agtccggcgg gccgtcgatg ccctcgatca ccggcaccgc
     1381 ccagtaggag ccggcggcac ggccgacgag ggcacccaag gcaccccatt cggcgagatc
     1441 ccggggccgc gtgcgcacga cgaagcggtg ggtgcctcgg cggttctcgt ccagatgcag
     1501 gccgtagcgc ggggtgcggc cggtgagcgc tgcggccagg gccgaggggc cgccctcgaa
     1561 gttggagcgg gcgccgagca cgctgttgga gtagatgacg acgccggtgt cgccataggc
     1621 ctgatgctcg ccgggtagcg gcggcatgat ggtctggtag ttgatgcagg tgtcggtcat
     1681 cagcacgcca agcgcctcga aggcgtcgat ggcgcggcgc tcgaggttgg ccatggcgtc
     1741 ggtctggccg agcttcctgt agtgcgcgaa gtcgatgccg cgcgggtcgg tgatggtcgg
     1801 gacgcggacc cgccggtcgg ccgctggcgc ctggcccagc cgctcgagga aggcgacgcc
     1861 cgcctcgccc aggctctcgg tgtcggccat gatgtgggcc tggtgcacct cgacgaggtc
     1921 ggccgcgtcg aaggcacggc cgacctgctg catgtgctga atcgcccatt gccggggggc
     1981 gccatgctcg ccccgcgcca tggcttcctc gatgtccgtc agccgcatgc gtccctccct
     2041 cgcaagaacg gtgctagcag cggcctcagg tgtcgacaag ccggcggggc cggttgccac
     2101 cgcgctcggg aatcccggtt tcctcgaaga agacgcgcgc cagggcggcg atgccctccc
     2161 tgatctccgc tttggtgggc aggccgtagc agagccgcat gcagtgcttc gcggcctccg
     2221 ggtcgcaggc gaccgttggc ccgggattga aggtgacgcc ggccgcccgg gcgggctcca
     2281 ccagcttccg gacgtcgatg tggtccggta acttcagcca gacgaagagg ccgcccttgg
     2341 gcgcggtgag ctccaccgac gtgccgaact cgcggtcgag cgcctcgatc agcgtgcgca
     2401 gcttgtcctc cagcgcgtcc gtgagctcgc gcagatggct gtcgaagtgc gttccgaaat
     2461 attcggcgat gaccatctgg tcgagtgctg ccgtgccgcc gtcggtcttg caggcgagca
     2521 tgcgcgccag gaccgagcgg tcggcggcga cgtagccgac gcggagcgcc ggggccagcg
     2581 acttcgagaa ggagccgata tggatgacct gcgtcggatc gagcgcccag agggccggcg
     2641 gggcgccgtc gcgccagatg atgtcggcgt agcactcgtc ctcgaagatg ggcacctcgt
     2701 aggcccgggc cacctcgagc agttgctggc ggcgctcgag gccaaggatg ctgccggtcg
     2761 ggttctggat ggtcgggatc gtgtagatgt atttcggccg gatgctcttg cgcttcagct
     2821 cttccaggac ggccgcgagc ttctcgacct cgatgccccc gtcgtcgagc ggggcgccga
     2881 cgggatggac gccgaggcgc tgcgcgcgcg agaacatggc ggaataggtg aacgcctcga
     2941 agatcaccgt gtcgcccggc tcgagcagca ggtcgttgac cagatcgatg ccctgcagcg
     3001 agcccgaggt gacgaggatg tcgtcggcgg tgcaggaggc gccacgcagc cggttcagct
     3061 tggccgcgac gaagtccctt agcccctcga agccgagcgg gttctggccc tggttgtaga
     3121 cggcgagatc ggcgccgtgc cgcctgatca ccgcggccgc ggcctcggcc agcgcttcgg
     3181 tcgggacctc gtcccggtcg ttgtggccaa cggcgaagtt gtatttcgcc ggcggtgacc
     3241 aggtttgtgc cggtgccggg agattgcgtc ggaagctcga cgtgtagcga aagccggcat
     3301 tttccctggt catcgtcccg tcccgataat gcgggccagt ggtcgggcag ttggcgacga
     3361 aaggcaatcc cgcttgtgac ctgcgacgac cttcggctag ggttttctga cagggcaggg
     3421 atggaggcgc tgggtattgg ccgaagggac gctcgaggtt cggatctggc ggggcgggga
     3481 cgagggtgcg ttcggcgcct atgcggtgcc ggcgcgcgag aaccagaccg tgctcgacgt
     3541 cgtgaccgag gtgcagcggc gcttcgagcc gtcgctcgcc tatcgcttct cctgccgggt
     3601 cggcgtctgc ggctcctgtg ccatgaccat caacggcacg ccgcgctgga cctgccgcac
     3661 gcacgtgaag cgggtcgcac ccgagggccg gctgaccatc gagcccctgc gcaacatgaa
     3721 gcgggtgaag gacctcgcct gcgacatgga cggcttcctc gggaaatggg ccagtgtcgg
     3781 caccttcgag ggcaccgcca cgcgcgccga cccgccggcc gccatcgacc cggcgagccc
     3841 ggcccgccgc gaggtcgacg ccgcgatcca gtgcatcaat tgtgcggtct gccatgcggc
     3901 ctgcgacgtg gtggcctggg acccgcatta cctgggaccg gccgccctca accgggcctg
     3961 gacgctctac aacgacgccc gtgtcgccga ccggcgggcg gtgctcgacg gtgccctgca
     4021 gccgggcggc tgccacacct gccacagcca tggcgcctgc accgagcact gtccggtcgg
     4081 gctcagcccg acggccagca ttgccggcct gaagcgggcc ggcctcgttt cgctgctcaa
     4141 gggtggtcgg tcgtgagcga ggccaggctc ttcctgctgc agcggctgac ggcgctggtc
     4201 atggcgccgc tcgtgctcgt tcatctggtg ctcatcgtcg tggcggtgca gggcgggctg
     4261 acggccggcg agatcctcgg ccgcacgcag ggcagtgtgt tctggggcct gttctacggt
     4321 attttcgtcc tggtggcggc gatccacgcg ccgatcggcc tgcgcaacgt gctgcgcgaa
     4381 tggaccggca tcggccggcg gccgatcgac tggctcatgg tcgccttcgg cgtcctgctg
     4441 ctggtgctgg gctggcgcgc ggtggtggcg gtcgtgggcg gcggggcatg aggcggggcg
     4501 ccggctggtg ggcggccatg gggcaccggc tctcgggcct ggcgctcgcg gccttcctgc
     4561 cgctgcactt cctcatgctg ggccgggcgc tgggcggcgc cgaggcgctg gacagctcgc
     4621 tgaagatcgc cgagctgccg ctggtcaagg tggcggagtt cggcctcgtc atcgcgcttg
     4681 ctgtgcatct ggcgttcggg ctccgggtgc tggcgatcga gctgctgccc tggccggcca
     4741 gggacgggct caggctgcgg ctggtctatc tcgggctcgc cgccacggtg ctggtcggca
     4801 tcgcctttgc cgtgagcgtg gtctgatgcg gatcgaggag agccgctgcg acatcctcat
     4861 cctgggctcg ggcggggccg ggctcttcgc ggcgctgcac gcggccaagg ccgacccggc
     4921 gctcgacatc gtcgttgcgg tcaaggggct gctcggcaag agcggctgca cgcggatggt
     4981 gcagggcggc tacaacgtgg cgctggccgc cgaggacagc atcgagcggc acttcatgga
     5041 caccatcgtc ggcggcaagt ggctgccgcg ccaggacctc gcctggcggc tctgtgtcgg
     5101 ggcggtggag cgggtgcgcg agctcgagaa cgagatcggc tgcttcttcg accgcaaccc
     5161 ggacggcagc ctgcaccaga aggccttcgc cgggcagagc ttcgatagga ccgcgcacaa
     5221 gggcgacctc accgggatcg agatcatcaa ccggctgatg gagcaggtgc gcagccgtgc
     5281 cgtgcggcgg ctcgaggagc accgggcgat cgagctgatc ccggcggcgg acggctccgg
     5341 gctggcgggc gtgctcctga tcgacatccg catgggcgag taccgcttcg tgcgggccaa
     5401 ggcggtgctg ctcgccaccg gcgccgggcc caccatgtat ctctatcaca cgccctcggg
     5461 cgacaagacc tgcgacgggc tcgccatggc gctgcgcctg ggcctcgcgc tgcgcgacat
     5521 ggagatggtg cagttccacc cgacggggct gctgggcggg ccgcacacgc ggatgaccgg
     5581 cacggtgctc gaggaggggc tgcgcggtgc cggcgggcat atcctcaatg gtggcaagga
     5641 gcgctttctc gccggctacg acccggcggg cgagcgggcg acccgtgacg tggtcagccg
     5701 cggcatctat gcggagatgc gcgccgggcg gacgacgccg atgggcgggg tctatatctc
     5761 gatggggcat ctgggtgccg agaaggtggc cggcatgttc cccggcatgg tcaagcgctg
     5821 cgccgattgc ggcttcgacc tcgccggcgg gctggtcgag gtggtgccga cggcgcacta
     5881 catgatgggc ggggtcgagt tcgagatcga cgcctcgacc gcggtgcccg ggctcttcgc
     5941 cgccggcgag gattgcggcg gcgtgcatgg cgccaatcgc ctaggcggca atggcgtggc
     6001 caactcgacc gtctatggcg gcattgccgg cgacagcatg gcggccttcg tcggccgcga
     6061 gggggtgtgg cgcgatccgg acaacagcgt catcgaggcc ggcatcgccc gggccgagcg
     6121 gccgttcggg caggcgccgg gcgatctcgc cgggctcagg gaccggctgg cgcaggtgat
     6181 gtgggacgat gtgggcgtga tccgcgacgc cctgggcatc gagcgcgggc tcgccgggat
     6241 cgcggattgc cgggcggcgc tcctcgccca gggtgtcgtc gatggcgacc ggcgcttcaa
     6301 cctgacctgg cacgactggc tcaatctcga gagcctgctc gacctctccg aggtcgtcgg
     6361 ccgggcggcg ctggcccggg acgacagccg gggtgcccat ttccgcgcgg atttccccga
     6421 gacgggggtg ctcgaaacct cggcctacac ccgcatccgc cgcgacggtg atgaacttcg
     6481 gctggacacg gccccggtcg ccttcgatat cgtcgagccg ggccagagcc tgatcgaggg
     6541 ggccgccggc gcaccgccgg cagaagcagc catagggagc gctgcatgat cgcgcacgag
     6601 caggtcgaga cggccgccta cgagatcatg aagaaggcgg cgatcgacat cccggaggac
     6661 tatctcgggg gggtgaaggg gatgatcgac ctcgagacgg gcgatctctc gagcttcgtt
     6721 ttgaagtcga tggtcgacaa ctgggaggcc gccaccgagg accggcggcc gatgtgcgcg
     6781 gatacgggcc tgccgcgcta ctacgtcaag gtcggcaacg aggcccgtgt cgccggcggg
     6841 ttctgcggtg tggaggaggc gctgcgctcg gccacggccc gggcgacgca cgacgtgccg
     6901 ctccggccga accgggtgca tccgctctgg cggaccgagc acaacaacaa tgtcggcatc
     6961 aacgcgcccg agatcgagtg gagcttcgag ccggacgtcg actggatcga cgtcaccacg
     7021 gtgcacaagg gcgggctctt cggcacggac taccggatgc tcttcccggg cgacggcatc
     7081 gacggcatca agagggtttt cctcgacacg ctgatcgcct tcggcaagcg cgggctcgcc
     7141 tgtcagccgg cgatcgtcgg cgtgggggtc ggcggctcga aggacatctg catgacgctg
     7201 ggcaagcagg cagcttgcct cagagtggtc ggcgacgcca acccggaccc gaggatcgcg
     7261 gccctcgagc aggagctgaa ggagctcggc aaccggatcg gcatgggtgc gatgggcttc
     7321 gtcggctcgt ccatggtggt cgattgccat gtcgaggtgg gctacaccca caccggcggc
     7381 atgccggtca gcgtgcacac cttttgcctg agctcgaggc gggcgacggc gcggctgcac
     7441 gccgacggac ggacgaccta ccgcaccgac cccaagtggt tcacccccta catgcgtcgg
     7501 gagacgatcg gatggccgag gagcgagccg agctccgcca gcgccggctg acgctgccgg
     7561 cgagtgctga ggcgattgcc gggctcgagc ccggcaacgt ggtctatctg gacggcgtgg
     7621 t
//
LOCUS       DAOTFI010000229         7584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11625_length_7584_cov_6.163634, whole genome shotgun sequence.
ACCESSION   DAOTFI010000229 DAOTFI010000000
VERSION     DAOTFI010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11625_length_7584_cov_6.163634"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..560
                     /locus_tag="P5558_19215"
                     /pseudo
     CDS             1..560
                     /locus_tag="P5558_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330183.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            876..2369
                     /locus_tag="P5558_19220"
     CDS             876..2369
                     /locus_tag="P5558_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRY26506.1"
                     /translation="MAFADLRAFMMHLESRGRLVRVREPVSPVLELTEIQTRLLAEQG
                     PAALFTKVEGTSMPVLVNLFGTVERVAWGIGREPHELRAVGEMLAFFRQPQPPDSFRE
                     ALEMAPLMKAALAMRPKHISRGSCQEIVWRGAEIDLGRLPVQTCWPGEPAPLITWPLV
                     VTRGPGTSREDGFNLGIYRMQVLERDRTIMRWLKHRGGAQQHRRWKEERQAPLPAAAV
                     LGADPGTTIAAVTPVPETMSEYQFAGLLRGQRVELVDCVSQPLKVPATAEIVIEGEVG
                     LDETADEGPYGDHTGYYNSVEPFPVFRVTAITMRRDPIYHSTFTGRPPDEPSVLGEAL
                     NDVFVPLLQQQFPEIVDFWLPPEGCSYRVAVVSIKKAYPGHAKRILMGVWSYLRQFMY
                     TKFVIVVDDDIDARSWIDVIWAISTRVDPGRDLTVVDGTPIDYLDFASPESGLGGKLG
                     IDATNKWPPETRREWGSKIRMAEEIVEKVTSKWQRYGLPGSGRSIWR"
     gene            2366..3223
                     /locus_tag="P5558_19225"
     CDS             2366..3223
                     /locus_tag="P5558_19225"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRY26507.1"
                     /translation="MTGGAQALDQEALLPAVRDAVRAAGRIALGDFGRPGRSWEKSPG
                     QLVTETDLAINRHLHAALGALLPVAAWLSEESVDDGRRHDADLVWVIDPIDGTRSFAA
                     GKPEFTISVALVAGEQPVLASLLNPATGEHFEALAGRGATLNGRALTVREPAPGAPAR
                     IVLSAGERRTRDFQAMLPEAGLSTIGSLAYKMALVAAGRFDAYFSWRYSHDWDIAAAM
                     LVLAEAGGMVSDRSGGALLLNRPAPRHAGLLAAPPFLHRDLVARSLRALASVAGPDGT
                     VLPTATRAG"
     gene            3283..4362
                     /locus_tag="P5558_19230"
     CDS             3283..4362
                     /locus_tag="P5558_19230"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HRY26508.1"
                     /translation="MHTLKAEQLERLAEATMRALGSAPEEARIVATHLVQANLSGHDS
                     HGVGMLPDYVRLVQAGLIVPNQEIVTLVDFGALLLFDARRGVGQRVMEATTAAAIERA
                     RSQGATVFGTRDCGHIGRVGHYAEQCAAAGMTGIFFVNVADHLAFQAPFGCSDARLGT
                     NPFAIAMPGANGPAAVLDMATSAIAFGKARVARNKGVPVPEGSVIDDAGRPTTDPRML
                     VDERKGALMAFGQHKGSGLAVFCELLGAALTGGRTIQSEEETSGGILNSVLGIVIDNA
                     RMGDPAAIIDEVESVKDWIRASPPAPGFDKVRLPGEPELESRASRGRSGIPLDAKSVD
                     DILGAAITAGVNPALVDATRAAYAA"
     gene            4419..5525
                     /locus_tag="P5558_19235"
     CDS             4419..5525
                     /locus_tag="P5558_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY26509.1"
                     /translation="MTDRIECVVVGAGVVGLAVAAALARSGREVIVLERHGLIGSETS
                     SRNSEVIHAGIYYPTGSLKARLCVAGKQALYRYCGERGIPHRNCGKLIVATSDGQLDE
                     LERIRLKAAENGVTDLEQWDAARARELEPELACTGALWSPSTGIIDSHSLMLAYQGDI
                     EDGGGMIAFNASLERARVTGEGFHLEVGGEAPMEIECAVLVNSAGLEAPNLARRIEGY
                     DPAHAPAAHYCKGNYYTLKGRSPFDRLIYPVPEAAGLGVHVTVDMGGQCRFGPDVEWV
                     ESIQYDVDPRRAEVFYAAVRKYWPGLQDGALEPGYSGIRPKLGPAGAPAADFVVQGAA
                     AHGVSGLVHLFGIESPGLTASPAIGEMVLATLEP"
     gene            5583..6575
                     /locus_tag="P5558_19240"
     CDS             5583..6575
                     /locus_tag="P5558_19240"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRY26510.1"
                     /translation="MTDGGEKAGAGYLVWDAHACLPLHEAADIRVLERHRRAGCSFVS
                     VNIGMDMNPLPQIMKVIASFRRQIAASPDMLVPVRTVADVETAHASGRLGVAFDLEGG
                     LPLQGRPDMVGLFAALGIRQMHLAYNRNNALGGGCYDEDVPLTPHGRAVVAAINDAGI
                     LMDCSHTGHRTSLDIMAASTAPVIFSHANPRALCADGRNITDEQIRACIATGGVVCVN
                     GVGRFLGDHAAGTPAILAHVDHLVQTYGDRHVGLGIDYSYPSGGLDDDPPGLDKDHWW
                     PPAHGYVGGLNIKIAPPEQLPGIVEGLVRLGYGERALRGILGLNMLEVARRVWG"
     gene            complement(6583..7077)
                     /locus_tag="P5558_19245"
     CDS             complement(6583..7077)
                     /locus_tag="P5558_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26511.1"
                     /translation="MRCGRYLAIVLLAGLVLAAGPARAAPAGAAGDVGLVVSGKVAGG
                     RQLLSMQDLGAAGVNEVVARVPWARGTARFAGLPLRHLLAMVSASGTSIEARDGRGNR
                     TNLSIEEALARNALIAISHEGEPIAEGGSGPYWLIYPEPEGAGRRSGLGPPPLPRLLE
                     LRIH"
BASE COUNT         1195 a         2603 c         2617 g         1169 t
ORIGIN      
        1 gacgcgcacg aaggcatcaa ggctgccgtc tcccctccgg cgcctgctgg cgccggaggg
       61 ctggcaagag cggtcgccgc gtcgtctctg ccttcatcgc caccggggtc accatggcgc
      121 ccgcgccatg gggtgggcca ccgccttcgc ccagaacgac cccgaagccg ccagccacca
      181 gtggcgccgt gtcgccgacc agctgcggcc gaagatgcag aagcttgctg ccttgatgga
      241 cgacgccgag cttgacgttc tggcctacat gacctttccg cagcaacacc gtgccaagct
      301 gcacagcgcc aatcccatcg agcgcctcaa ccgcgagatc aagcgccgca ccgacgtcgt
      361 cggcatcttc ccaaatgagg ccgccatcac ccggggtccc catgcggcct gccgcatggg
      421 gtggcgcccg cctggtcggc gccctgctcc tcgagcaaaa cgacgagtgg gccgtccagc
      481 gcgcccgcta catgaccctt gagaccatcg cccagactgg cgatgatgct gcagtcagct
      541 tggccgacat gaccggctga ccaaacaccg gccgaggccg gagaacacct tgggcacagc
      601 tcagctacac cactccatgg gacacgaccg gtgccagcgc tcgcttgatg gcgcttcaag
      661 tcaggcctgc attgtgtcag ctggcgattc cagtcagtct cgcccgaccc ggaagacggg
      721 gggccggtag ccattttcgg tccggtcttc acccttcgcg tgagaagcgt tgatgttgtc
      781 gacagtcctg ccgatgcatg tcggtccagt cccgatcccc accagggagg atcgttcgct
      841 agcacgcacg atccgccggc gggacgtctg atcccatggc atttgcggac ctccgggcct
      901 tcatgatgca tctcgaatct cgtggccggt tggtccgtgt tcgagagccc gtttcgccgg
      961 tgctcgagtt gaccgagatt cagacccgcc tccttgccga gcaaggcccc gcggcgctgt
     1021 tcacgaaggt cgagggaacg agcatgcccg tgctcgtcaa cctctttggc acggtcgagc
     1081 gggtggcctg gggcatcggc cgcgagccgc atgagctgcg cgccgtcggc gagatgctcg
     1141 cgttcttccg ccagccccag ccgccagaca gcttcaggga ggcgctcgag atggcgccgc
     1201 tcatgaaggc ggccctagcc atgcggccga agcacatctc ccgtggtagc tgtcaagaga
     1261 tcgtctggcg cggcgccgag atcgatctcg gccgcctgcc ggtccagacg tgctggccgg
     1321 gcgagccggc gccgctgatc acttggccac tggtggtgac cagggggccg ggcaccagcc
     1381 gtgaggacgg cttcaatctc ggcatctacc ggatgcaggt cctcgagcgc gaccggacca
     1441 tcatgcgctg gctgaagcat cgcggcggtg cccagcagca tcgccgctgg aaggaggagc
     1501 ggcaggcgcc cctgccggcg gccgccgtcc tgggcgccga tccgggcacg acgatcgctg
     1561 cggtgacgcc ggtgccggag accatgtcgg agtatcagtt cgccggcctc ctgcgcggcc
     1621 aaagggtcga gctcgtcgac tgcgtcagcc agccgctcaa ggtgccggcg accgccgaga
     1681 tcgtcatcga aggcgaggtc ggcctcgacg agaccgccga cgagggtccc tatggtgatc
     1741 acaccggcta ctacaactcg gtcgagccct ttcccgtctt tcgcgtcacc gccatcacca
     1801 tgcgtcgtga tccgatctac cactccacct tcaccggccg cccgccggac gagccttccg
     1861 tgctcggtga ggcgctgaac gacgtcttcg tgccgcttct gcagcagcag ttccccgaga
     1921 tcgtcgattt ctggctgccg cccgagggct gctcctatcg ggtcgcggtc gtgtccatca
     1981 agaaggccta tccgggccat gccaaacgga tactgatggg cgtctggtcc tatctgcggc
     2041 agttcatgta tacgaagttc gtcatcgtcg tggacgatga catcgacgcc cgctcgtgga
     2101 tcgatgtgat ctgggcgatc tcgacccggg tcgaccccgg gcgggacctg actgtcgtcg
     2161 acggcacgcc catcgactat ctcgactttg cctcacccga atccggtctg ggcggcaagc
     2221 tcggcatcga tgcaacgaac aagtggccgc ccgagaccag gcgcgaatgg ggcagcaaga
     2281 tccgcatggc cgaggaaatc gtcgagaagg tgacgagcaa atggcagcgc tacggcctgc
     2341 ccggcagcgg ccggtcgatc tggcgatgac gggcggagca caggctctcg accaggaggc
     2401 actgttgccg gcggtgcgcg acgcggtgcg ggccgcaggc cggatcgccc tgggtgattt
     2461 cggccggccg ggcagaagct gggaaaaatc ccccgggcaa ctcgtcaccg agaccgacct
     2521 cgccatcaac cggcatctgc atgcggcgct cggcgcgctc ttgcccgtcg cggcctggct
     2581 ctcggaggag agcgtcgatg acggccgccg ccacgatgcc gacctggtct gggtgatcga
     2641 cccaatcgac ggcacgcgca gcttcgccgc cggcaagccg gaattcacca tcagcgtcgc
     2701 cctggtggcc ggcgagcagc cggttctcgc cagcctcctc aacccggcga caggcgagca
     2761 tttcgaggcg ctggccgggc gcggcgccac gctgaacgga cgggccctga cggttcggga
     2821 gccggcgccc ggcgcgccag cgcggatcgt gttgagcgcc ggcgaacggc ggacccgcga
     2881 cttccaggcc atgctgcccg aggccggcct cagcacgatc ggctcgctcg cctacaagat
     2941 ggcgctggtc gctgccggcc ggttcgacgc ctatttctcc tggcgctaca gccatgactg
     3001 ggacatcgcg gcggccatgc tggtgctggc ggaagccggc ggcatggttt ccgaccgctc
     3061 gggcggcgcc ctgcttctga accgcccggc gccacgccat gccgggctgc tcgccgcccc
     3121 gccgttcctg caccgtgatc tcgttgcccg ctcgctacgg gcgctcgcca gcgtcgccgg
     3181 acctgacggg accgtcttgc cgacagcgac acgggcggga taagcgaagc gcccggtagc
     3241 gcagcaccgg tgtccgttgt caacgcaagc ttagggaacg agatgcacac tctgaaggcc
     3301 gagcagctgg agcgactggc cgaggccacc atgcgggcgc tcggcagcgc gcccgaggaa
     3361 gcccggatcg tcgctaccca tctggttcag gccaatctca gcggccacga cagccatggg
     3421 gtgggcatgc tgccggacta tgtcaggctc gtgcaggcgg ggttgatcgt gcccaaccag
     3481 gagatcgtga ccttggtcga tttcggcgcc ctcctgctct tcgacgcccg gcgcggggtc
     3541 gggcagcgcg tgatggaggc gacgaccgcg gccgcgatcg agcgcgcccg gagccagggg
     3601 gcgaccgtct tcggcacccg tgactgcggc catatcgggc gggtcgggca ctacgccgag
     3661 cagtgtgccg ccgccggcat gaccgggatc tttttcgtca atgtggctga ccacctggcc
     3721 ttccaggcgc ccttcggctg cagcgacgca cggctcggca ccaatccctt cgccatcgcc
     3781 atgccgggtg ctaacggccc tgccgcggtg ctcgacatgg ccaccagcgc catcgccttc
     3841 ggcaaggccc gggtggcgag gaacaagggg gtgccggtgc cggaaggctc ggtcatcgac
     3901 gacgccgggc gaccgaccac cgatcccagg atgctcgtcg acgagcgcaa gggtgcgctc
     3961 atggccttcg gccagcacaa gggctccggc cttgcggtct tctgcgagct gctgggtgcg
     4021 gcgctgaccg gtggtcggac gatccagtcg gaggaggaga cgtccggcgg catcctgaac
     4081 agcgtgctcg ggatcgtgat cgacaatgcc cgcatgggcg acccggcggc catcatcgac
     4141 gaggtggaat ccgtcaagga ctggatccgc gcctcgccac cggcgcccgg cttcgacaag
     4201 gtgcgcctgc ccggcgagcc ggagctggaa agccgcgcca gccgtggcag gtcgggcatt
     4261 ccgctcgatg cgaagagcgt cgacgacatc ctgggggcgg cgattacagc cggcgtcaat
     4321 ccggcgctcg tcgacgcgac ccgtgccgcc tatgcagcct gaggacagat gcccatgacc
     4381 tgccagaccg aacaggcgag tgccggaggc cgagcggcgt gaccgatcgg atcgaatgcg
     4441 tggtcgtggg cgccggcgtg gtcggcctcg ccgttgccgc cgcgctggcg cggtcggggc
     4501 gtgaggtgat cgtgctcgag cgccatgggc tcatcggcag cgagacgagc tcgcgcaaca
     4561 gcgaggtcat ccatgccggc atctactacc cgaccggcag cctcaaggcg cggctgtgcg
     4621 tggccggcaa gcaggcgctc tatcgctact gtggggagcg ggggatcccg caccgcaact
     4681 gcggcaagct gatcgtcgca accagcgatg gccagctcga cgagctcgag cgcatccgcc
     4741 tcaaggctgc cgagaacggt gtcaccgatc tcgagcaatg ggatgccgcc agggccaggg
     4801 agctcgagcc ggagctcgcc tgcaccggag cgctctggtc gccatccacg ggcatcatcg
     4861 acagccacag cctgatgctc gcctaccagg gcgacatcga ggatgggggc ggcatgatcg
     4921 ccttcaatgc cagcctggag cgggcccgcg tgaccgggga gggcttccat ctcgaggtgg
     4981 gcggcgaggc gccgatggag atcgaatgcg cggtgctggt caacagcgcc gggctggagg
     5041 ccccgaatct ggcacggcgg atcgagggct acgatccggc gcatgcgccg gccgcgcact
     5101 actgcaaggg caactactac acgctcaagg gccgcagccc cttcgaccgc ctgatctacc
     5161 cggtgccgga agcagccggg ctcggcgtgc acgtcaccgt cgacatgggc gggcaatgcc
     5221 gcttcggccc ggatgtcgag tgggtcgaga gcatccagta cgatgtcgac ccgcgacggg
     5281 ccgaggtctt ctatgcggcc gtccgcaagt actggccggg ccttcaggac ggtgccctcg
     5341 agcccggcta ttcgggcatc cgcccgaagc tcggcccggc cggtgcgccg gcagcggatt
     5401 tcgtcgtcca gggggcggcg gcccatggcg tttcgggcct cgtgcacctc ttcggcatcg
     5461 agagcccggg gctgacagcc tcgccggcca tcggcgagat ggtgctggcg acactcgagc
     5521 cgtgagcggc cgaggctcga atgcggcaac ccgtggacca gccagttgat ggagatgccg
     5581 gcatgacgga cgggggggaa aaggcagggg cgggctatct cgtctgggat gcgcatgcct
     5641 gcctgccttt gcacgaggcg gccgacatcc gggtcttgga gcggcaccgc cgggcgggct
     5701 gcagcttcgt ctcggtcaat atcggcatgg acatgaaccc cctgccgcag atcatgaagg
     5761 tcatcgcgag ctttcgccgg cagatcgcgg cgtcgccgga catgctcgtg ccggtgcgca
     5821 cggtggcgga tgtcgagacg gcccatgcca gcggccggct cggcgtcgcc ttcgacctcg
     5881 agggcggcct gccgctgcag ggccggccgg acatggtcgg gctctttgcg gcgctgggta
     5941 tccggcagat gcacctcgcc tacaaccgca acaacgcgct gggcggcggc tgctacgacg
     6001 aggacgtgcc gctcacgccc catggccggg ccgtggtggc ggcgatcaac gacgccggca
     6061 tcctgatgga ctgctcgcat accgggcacc ggaccagcct cgacatcatg gcggcatcga
     6121 ccgcgccggt gatcttcagc cacgccaacc cgcgggccct ctgcgccgac ggccgcaaca
     6181 tcacggacga gcagatccgc gcctgcatcg ccacgggcgg cgtcgtctgc gtcaacgggg
     6241 tcggccggtt cctcggcgac cacgcggccg gaaccccggc gatcctcgcc catgtcgacc
     6301 acctcgtgca gacctatggc gaccggcatg tcggcctcgg catcgactac agctacccgt
     6361 cgggcggtct cgacgacgac ccgccggggc tcgacaagga ccactggtgg ccgcccgcgc
     6421 acggctatgt gggcgggctg aacatcaaga tcgccccgcc ggagcagctg ccggggatcg
     6481 tcgaggggct ggtccggctc ggctatggcg agagggcatt gcggggcatt ctcgggctca
     6541 acatgctgga ggtggcgcgg cgcgtctggg gctgaccggg cttcagtgga tgcgcagctc
     6601 caggaggcgg ggcaggggcg gcgggcccag cccggaacgc cgcccggcgc cctcgggctc
     6661 gggatagatc agccagtagg ggccgctgcc gccttcggcg atcggctcgc cctcgtggct
     6721 gatggcgatc agggcgttcc ttgccagggc ctcctcgatg gagaggttcg tccggttgcc
     6781 gcgcccgtcg cgggcctcga tgctcgtgcc ggaggcactt accatggcga gcaggtggcg
     6841 cagcggcagg ccggcgaagc gggctgtgcc cctcgcccat ggcaccctcg cgacgacctc
     6901 gttcacgccg gcggcaccga gatcttgcat gctcagaagc tggcggccgc cggccacctt
     6961 gccggagacg acgagcccga catcgccagc cgcacctgcc ggagcagcgc gggcaggacc
     7021 cgcggcgagg acgaggcccg ccagcagcac gatcgccagg taccggccac agcgcatctt
     7081 cctcgagcca cccttcacga aaccggcaac cggcgccggt gaaggggaga ggatgcacgc
     7141 gccgggttaa catgccgcta atcgccgacc gctgagagaa tattatccta tttcgcccgt
     7201 cctgtcaagg ggcgcgggtt cgctcggcaa atcgcgcttt tcgggttcct tcgcagccgg
     7261 gcagacggcc ggaggcccgg aatcgctggc ccgggcgctc gggttcgttc gtcgaaagcc
     7321 cgggttcgct cgtcgaatcg ccgggttcgc tcgtcgattc cgtgggttca ttcgtcgatt
     7381 cggcgggttc gttcgccctg aaatgtcgca gattccgcga catcccggga ttcctgtcgc
     7441 agaatacgcg acatcagggc gcgagatgtc gcggaatccg cgacaaccca agcgactctg
     7501 tcgcggaatc cgcgacaacc cgagcgccgc tgtcgcggaa tctgcgacaa cccgggctgg
     7561 aagagcgggg gaactgagtt cccc
//
LOCUS       DAOTFI010000230         7454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11886_length_7454_cov_6.038654, whole genome shotgun sequence.
ACCESSION   DAOTFI010000230 DAOTFI010000000
VERSION     DAOTFI010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11886_length_7454_cov_6.038654"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="P5558_19250"
     CDS             <1..392
                     /locus_tag="P5558_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HRY26512.1"
                     /translation="MIPTSTGAAKAVGLVLPELKGRLDGVAIRVPTQNVSLVDLTFDA
                     GRATTVDEINGAFKAAANGPLKGVLAVSDEPLVSIDYNHQPASSTVDLQSTKVVDGSL
                     VRVASWYDNEWGFSNRMVDTAALMGRL"
     gene            487..954
                     /locus_tag="P5558_19255"
     CDS             487..954
                     /locus_tag="P5558_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HRY26513.1"
                     /translation="MVRYSLLVMVMVALASLWPERHGVAAAQATVEVVIDPATGQPRY
                     EPPILYIEPGDSVLFKSAGSSHASRAIPGMQPEAADPWWGQVGTDLIVTFDEPGIYGH
                     KCGANYRLGLVGLIVVGDAPVNLASARAVPHPPAAAAAFDELFAELEAHYRAK"
     gene            complement(939..1895)
                     /locus_tag="P5558_19260"
     CDS             complement(939..1895)
                     /locus_tag="P5558_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRY26514.1"
                     /translation="MPVDRPAAASAMRLPFHELVELVHTAGGLEDIARRQPERLRWAE
                     QLLRLPEVWRLTRGQGVRVAILDTGIDADHPDLQGAIEKMRDFTGEGVEDLNGHGTHC
                     AGVVAARANDVGFVGTAPLSSLLIGKVLANNGSGAIGAVAEAVDWAVDEGADIISMSL
                     GSTTDSPELHRAVHAALAKGVIIVCAAGNTGELFANNIGFPGRYGSVITVAAHDRYGQ
                     PSGFSSRGASIDFMAPGSDIWSTYRQGGYAKLSGTSMATPFVAGLAALVLARHRLGGS
                     HVTPIRNCEDMREHLVRMAAHPGHHDSARGHGPLLPFAYFAR"
     gene            complement(1949..3679)
                     /locus_tag="P5558_19265"
     CDS             complement(1949..3679)
                     /locus_tag="P5558_19265"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY26515.1"
                     /translation="MHALVRLRAIVRSGSALLLLAALTPSGLHAQETKVTGEAAASVS
                     ATELQAAIDAIRRQVAEQKAERESSGGGELAAELRAARDAIAELTRSTGLLRDERDAL
                     MAELQAQRETARGLQASVAAAGQREVEAREALAQALEEAKVARAEVEAERVAAVELAS
                     QDADRIAALEAELATAATSRDATAADLEAARAALEEQSRAHQAKVAELEASLTAAGDA
                     ALELQVRLDQEGRLLAAATQARDAAEGRAIAAEQSLAQANDATTQLELELVALREVAS
                     NSVIEVQGIGEQLLASLRENDELGAALEQARASRAALEQELAAMRQDIQIYVSELAAM
                     RDGGAEGPVALVASDAIVETLDAELATAREQMRALNEELIARDKQLVALGEAGDADAL
                     AQRLGLLERELETAQAENARLVDELAGLRAADQPETPEVLVASAAANGNAESVLGQLD
                     AVDTGDGWWMTVPDGLVFAPGSGNLAPGAEQALAQVAALLGYFDGAPVRIVGHTDSYG
                     DAAVNRELSLLRANAVRNVLVADFGVDPKRVETEGLGEDRPVSSNGTIEGRRANRRVE
                     IYVRRGPLDG"
     gene            complement(3805..4260)
                     /locus_tag="P5558_19270"
     CDS             complement(3805..4260)
                     /locus_tag="P5558_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRY26516.1"
                     /translation="MAALSLLLHNLAATLWVGGMFFAYVILRPSLAETAAAERLALWQ
                     RVFTRFFPWVLAAIAVLLATGYGLLFLVLGGFGGAGLHVHVMHLTGWLMFLLFFHLYF
                     VPWRRYRRALADGNSGDAARWLGQIRLIVLVNLVLGLLTVAVGASGRAW"
     gene            complement(4297..5151)
                     /locus_tag="P5558_19275"
     CDS             complement(4297..5151)
                     /locus_tag="P5558_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY26517.1"
                     /translation="MRTILAHLEISPVLDSVLASTLIVARRYASYIEGFHMRPGQPDV
                     IAAGADGFVAAAPDLVAGFEREARERSERATERFQAFMAENSLKRAAEVEGAGLAADW
                     RVETASGPTALGNRGRIFDLIVVGRPLADQVTPSIAALEAALFDSGRLLLVAPPASPT
                     AFGKHVVIAWNGSTETARTIGLAEQFLVDAERVTVVTVEKGSVPGPSGAELTRNLQRR
                     GIEAEFRDVQPGDRAVGKAILDETAAVGGDLLIKGAYTQSRLRQMIFGGATSHILAEA
                     NLPVLMAN"
     gene            complement(5316..6029)
                     /locus_tag="P5558_19280"
     CDS             complement(5316..6029)
                     /locus_tag="P5558_19280"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRY26518.1"
                     /translation="MLTLAMPTEATEPESVAEAALLAARSGADLLLLPLHGERAAEPC
                     DGPLAAGMGERAGEARIAILFTYAEACSGNTHLALQLVQADGRATANCRAAHLSKAAL
                     QAGWRPGDWLTIALLGGLRVGLMGGLDPLAPEVGRALSALGAELLVGALVGSPACPEE
                     ALQALATVRALENGAAVCLFGGPGSCAVANADGRPAERAMQAGLSLFRVHAGERHGTV
                     ARRPELYRRLQMAADTVVE"
     gene            6189..6884
                     /locus_tag="P5558_19285"
     CDS             6189..6884
                     /locus_tag="P5558_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY26519.1"
                     /translation="MKVLVIEDDVAAASYMVRGLEESGHVASVAHDGRDGLMLAASQT
                     FDVLVIDRMLPGLDGLGVIRTLRATGNRTPVLVLSALGNVDDRVEGLKVGGDDYLVKP
                     YAFSELLARLEALTRRGQPNAEQAPTTQLRYSDLSMDLLGRRVERRGKRIDLQPREFR
                     LLEVLLRHPEQVLTRTMLLEKVWDYRFDPQTNIIDVHISRLRQKIDRDFEPPLIQTVR
                     GSGYALRIPDQTA"
     gene            6982..>7454
                     /locus_tag="P5558_19290"
     CDS             6982..>7454
                     /locus_tag="P5558_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26520.1"
                     /translation="MERQTEETIEAELTGLREQYRDLGIFGLERIIAQRAAEEKHLAS
                     IYLLIDKRGVPIAGNLPAWPTDVETVSNRFRFSLDLPGSSGPRRFLGRSVELDQGFLL
                     VARDIEDKLRTQTLLVNAIALGSGLMLVFGVIGGFVMSRWMLTRIETINRATGQI"
BASE COUNT         1161 a         2672 c         2464 g         1157 t
ORIGIN      
        1 cgatgatccc gacctcgacc ggtgctgcca aggccgtcgg cctcgtcctg ccggagctca
       61 agggcaggct cgacggggtc gccatccgcg tgccgaccca gaacgtcagc ctcgtcgacc
      121 tcaccttcga tgccggccgc gccaccacgg tcgacgagat caacggcgcc ttcaaggcgg
      181 ccgccaacgg cccgctgaag ggtgtcctgg cggtgagcga cgagccgctc gtctcgatcg
      241 actacaatca ccagccggcc agctcgacgg tcgatctcca gtcgaccaag gtcgtcgacg
      301 gctcgctcgt cagggtcgcc agttggtacg acaatgaatg gggcttctcg aaccggatgg
      361 tggatacggc tgccttgatg ggtcgcctct gaggcacctg cccgcacgca tctgtgatcc
      421 gcctcgcctg gtggacaggg cgtgatgggc cacattagaa acgtcggttc ccacgcgagg
      481 gacttggtgg tgcggtactc gctcctggtg atggtgatgg ttgctttggc gtcgctttgg
      541 cccgagcgcc atggtgttgc ggccgcgcag gcgaccgtcg aggtcgtgat cgacccggcc
      601 accggccagc cgcgctacga gccgccgatt ctctacatcg agccgggtga ttcggtgctc
      661 ttcaagagtg ccggaagctc gcatgccagc cgggccattc ccgggatgca gcccgaggcg
      721 gccgatccct ggtggggcca ggtcggcacg gatttgatcg tgaccttcga cgagccgggc
      781 atctatggcc acaagtgcgg cgcgaactac cggctcgggc tcgtcgggct gatcgtcgtc
      841 ggcgacgccc ccgtcaatct cgccagtgcc cgagccgtcc cgcacccgcc ggccgctgcg
      901 gcggccttcg acgagctctt cgccgagctc gaggcgcact accgggcgaa ataggcgaac
      961 ggcagcagcg gcccgtgccc ccgagcactg tcgtgatggc cgggatgggc ggccatgcgc
     1021 accagatgct cgcgcatgtc ctcgcagttg cgaatgggcg tcacgtggct gccacccagc
     1081 cggtgccggg cgagcaccag ggccgcgagg ccggcgacaa atggtgtcgc catcgacgtg
     1141 ccgctgagct tggcgtagcc gccttgccgg taggtcgacc agatgtcgct acccggcgcc
     1201 atgaagtcga tcgaagcacc gcgcgacgag aagccgctcg gctggccgta tcgatcatgg
     1261 gcggcgacgg tgatcacgct gccgtagcgc ccgggaaaac cgatattatt ggcgaagagc
     1321 tcgcccgtgt tgccggcggc gcagacgatg atgacaccct tcgccagcgc cgcatgaact
     1381 gcccgatgga gctcggggct gtccgtggtc gagccgagcg acatcgagat gatgtccgcg
     1441 ccctcatcca cggcccagtc caccgcttcc gccaccgccc cgatggcgcc cgatccgttg
     1501 ttggcgagca ccttgccgat gagcagcgag ctcaggggtg cggtgccgac gaagccgaca
     1561 tcgttcgccc gcgcggcgac gacgccggca caatgcgtgc cgtggccatt gaggtcctcg
     1621 acgccttcac ccgtgaaatc ccgcatcttc tcgatggcgc cctgcaaatc gggatgatcc
     1681 gcatcgatcc ccgtatcgag gatcgcgacc ctgacaccct ggccgcgtgt caggcgccag
     1741 acctcgggca ggcggagcaa ctgctcggcc cagcgcagcc gctcgggctg acgccgggcg
     1801 atgtcctcca ggccgccagc cgtatggacg agctccacca gctcgtggaa aggcagccgc
     1861 atggcgctgg cggcggctgg tcggtcgacg ggcatgggcg gacctcacga agccgatggc
     1921 acaagtgagg tttttatacc taggttgatt atccgtcaag cggtccccgc cggacgtaga
     1981 tctcgacccg ccggttcgcc cggcggccct cgatggtgcc attggacgaa acgggccggt
     2041 cctcccccag cccctccgtc tcgacccgct tcgggtcgac gccgaaatcg gccaccagga
     2101 cgttcctgac cgcatttgcc cgcagcaagg acagctcgcg attgaccgcc gcatcgccat
     2161 agctgtccgt gtggccgaca atgcgcaccg gtgccccatc gaaatagccg aggagtgcgg
     2221 caacctgcgc cagcgcctgc tcggcgccgg gtgcgaggtt cccgctgccg ggcgcgaaga
     2281 cgaggccatc gggcacggtc atccaccagc catcaccggt atcgaccgca tcgagctggc
     2341 cgagaacgct ctcggcgttc ccgttggcgg cagcggaggc gacgaggacc tccggcgtct
     2401 cgggctgatc cgcggcgcgc agcccggcca gctcgtccac cagccgagcg ttctccgcct
     2461 gcgcggtttc cagctcccgc tccagcagcc cgaggcgctg ggccagcgcg tcggcgtcac
     2521 cagcctcgcc gagggcgacg agctgcttgt cccgggcgat gagctcctcg ttgagggcgc
     2581 gcatctgctc gcgcgccgtc gccagctccg catcgagcgt ctcgacgatc gcgtcactgg
     2641 cgaccagggc caccggcccc tccgcgccac cgtcgcgcat cgctgccagc tccgagacat
     2701 agatctggat atcctggcgc atggccgcga gctcctgctc gagcgccgcc cggctcgcac
     2761 gggcctgctc gagggcggcg cccagctcgt cgttctcccg cagcgacgcc agcagctgct
     2821 cgccgatccc ctgcacctcg atgaccgagt tgctcgccac ctcgcgcagg gccacgagct
     2881 ccagctccag ttgggtggtg gcgtcgttcg cctgagcaag cgactgctcg gcggcaatcg
     2941 cccgcccttc ggcggcatcc cgtgcttgcg tagccgccgc gagcagccgg ccctcctggt
     3001 cgagccgcac ctgaagctcg agggccgcgt cgccagccgc cgtcaggctc gcctcgagct
     3061 cggccacctt tgcctgatgc gcccgggatt gctcctccag tgccgcccgg gctgcctcca
     3121 ggtccgccgc cgtggcgtcg cgtgacgtgg cggccgtcgc cagctccgcc tcgagcgcgg
     3181 cgatccggtc ggcatcctgg ctggcgagct ccaccgccgc cacccgctcg gcttcgacct
     3241 cggccctcgc taccttcgcc tcctcgagcg cctgcgccag cgcctcgcgt gcctcgacct
     3301 cgcgctgccc ggccgcggca acgctcgcct ggagaccacg agccgtctcg cgctgcgcct
     3361 gcagctcggc catcagggcg tcccgctcgt cccgcaggag gccggtgctg cgcgtcagct
     3421 cggcgatggc gtcgcgcgcg gcccgcagct ccgccgccag ctcgccgccg cccgagctct
     3481 ctcgctccgc cttctgctcc gccacttggc gccggatggc atcgatggcg gcctgcagtt
     3541 cggtcgccga aacactcgcc gccgcctcgc ccgtgacctt cgtttcctgc gcgtggaggc
     3601 cggacggggt gagggcggcg agcagcagca gcgccgagcc cgaacggacg atcgcacgaa
     3661 gcctgacgag cgcatgcatg ggcatttccc ctgggggcgg tcatggcagc cgccccgctc
     3721 tctacaactg gatgactcga taacgcttag tggccggggc ttcaatcgac cggcatcggc
     3781 accgctcgat catattccgg cacatcacca ggcacgaccg ctggcgccca ccgccacggt
     3841 gagcagcccg agcaccaggt tgaccaggac gatcagccgg atctggccga gccaccgcgc
     3901 ggcgtcgcca gagttgccat cggccagggc gcgtcggtaa cgccgccacg gcacgaagta
     3961 gagatggaag aagagcagga acatcagcca gccggtcaga tgcatgacgt ggacatgcag
     4021 cccggcaccg ccgaatccgc cgagaacgag gaagagcagc ccgtatccgg tcgccagcag
     4081 cacggcgatg gcggcgagaa cccagggaaa gaagcgggtg aacacgcgtt gccagagcgc
     4141 cagccgctcc gccgcggccg tctcggcgag gctcggccgc aagatgacat aggcgaagaa
     4201 catgccgccg acccacaggg tggcggcgag gttgtgcagc agcagcgaca gcgccgccat
     4261 gttcgcgatc cccgatatcg tggtcgaagc cggcgctcag ttggccatca gcaccggcag
     4321 gttggcctcg gcgagaatgt gactcgtcgc cccgccgaag atcatctggc gcaggcggct
     4381 ctgcgtgtag gcgcccttga tcagcaggtc accacccacg gccgccgtct cgtcgaggat
     4441 cgccttgccg accgcccggt caccgggctg cacgtcgcgg aactcggcct cgatcccgcg
     4501 gcgctggagg ttgcgggtga gctcggcacc gctcggcccc ggcacgctgc ccttctcgac
     4561 ggtgacgaca gtgacccgct ccgcatccac caggaactgc tcggccaggc cgatcgtccg
     4621 cgcggtctcg gtgctgccgt tccaggcgat gaccacatgc ttgccgaagg ctgtcggcga
     4681 ggccggaggg gcgaccagca gcagccggcc gctgtcgaag agggccgcct cgagcgccgc
     4741 gatgctcggc gtcacctgat ctgccagggg gcgcccgacc acgatgagat cgaagatccg
     4801 cccccggttg ccgagtgccg tcggcccgct cgccgtctcg acccgccaat cggcggcgag
     4861 gccggcaccc tccacctcgg cggcacgctt gagtgagttc tcggccatga acgcctggaa
     4921 gcgttcggtg gcacgttcgg acctttccct ggcctcgcgc tcgaagccgg cgaccagatc
     4981 cggtgcggcc gccacgaagc cgtcggcacc cgccgcaatc acatccggct gacccggccg
     5041 catgtgaaac ccctcgatgt acgaggcgta gcgtcgggcg acgatcaggg tgctcgcgag
     5101 caccgagtcg aggacagggc tgatttcgag atgggccaga atcgtccgca tcttggcctg
     5161 ctccggctgt tgcaattttc ttgggcgtgc gcgggaccgt gacgccgcaa gcgttgcccg
     5221 acgccgctcg acacgtcaat gatgtcgatg cggcgagcct cggcgaacat agcatgactt
     5281 cagcgtcact gcgatggatc gctgccggcc gaggcctact cgactaccgt gtccgccgcc
     5341 atttgcagcc gccgatagag ctccggacga cgggctaccg tgccgtgacg ctcgcccgca
     5401 tgaacccgaa agaggctcag gccggcctgc atggctcgct ccgccgggcg gccatcggcg
     5461 ttcgccacgg cgcagctgcc gggaccaccg aagaggcaga ctgctgcgcc gttctccagc
     5521 gcccggacag tggccagggc ctgaagcgcc tcctccgggc aggcgggcga acccaccagg
     5581 gcgccgacga gcagctcggc gccgagcgcg ctcagcgccc ggccgacctc gggtgcgaga
     5641 ggatcgagac cgcccatcag cccgacccgc aagccgccaa ggagcgcgat ggtcagccag
     5701 tcgccgggtc tccagccggc ctgcagggca gccttcgaca gatgggcggc gcggcagttc
     5761 gccgtcgcac ggccatccgc ctggacgagc tgcagggcca gatgcgtgtt cccgctgcac
     5821 gcctcggcat aggtgaagag gatggcgatg cgcgcctcgc cggcccgttc gcccatcccg
     5881 gccgccagcg gcccgtcaca gggctcggca gccctctcgc cgtgcagggg cagcaacagc
     5941 agatcggcgc cgctccgggc ggccagcagg gccgcctccg ccacggattc cggctcggtt
     6001 gcctcggtcg gcatggccag cgtcagcatt gccgaaatcc gggccgaaag tggaaaaagc
     6061 aagacgtgac agcgatttca tgtcgcagcc acgcggcaga gaagaacctt tcccatcttg
     6121 cggggcgaga acgctgtttc gctgtcgaac cgctggcggt cactgcaaat cgaggccata
     6181 tgtcgggtat gaaggttctg gtcatagaag atgatgtcgc cgcggcgtcg tacatggttc
     6241 gtgggctcga ggaaagcggc catgtcgcca gtgtcgccca cgatggtcgc gatggtctga
     6301 tgctggcggc gagccagacc ttcgatgtgc tggtgatcga ccgcatgctg ccgggcctgg
     6361 acgggctcgg tgtcatccgc acgctccgcg ccaccggcaa ccgcacgccc gtgctcgtgc
     6421 tatcggcgct ggggaatgtc gacgaccggg tcgaggggtt gaaggtcggc ggcgacgact
     6481 acctggtcaa gccttatgcc ttcagcgagc tcctggcccg tctggaggcg ctcacccgcc
     6541 gcggccagcc caacgccgag caggcgccga ccacgcagct gcgctactct gacctgtcga
     6601 tggatctgct cggccgccgc gtcgagcgcc ggggcaagcg gatcgacctg caaccccggg
     6661 aattccggct cctcgaggtg ctgctccggc atccggagca ggtgctgacc cgaaccatgc
     6721 tgctcgagaa ggtctgggac tatcgcttcg acccgcagac caacatcatc gatgtccata
     6781 tcagccgact ccgccagaag atcgaccgcg atttcgagcc accgctcatc cagactgtcc
     6841 gagggtcagg ctacgcgctt cgcattcccg accagaccgc ttaattcctc caccttccgc
     6901 ctggcggtcg gctatttcgc gatcttcagc gtctcggcgc tggcgctgct cagcttcgtc
     6961 tatatcgccg ccgtcgattt catggagcgc cagacggagg agaccatcga ggccgagctg
     7021 acggggctca gggaacagta ccgggatctc ggcatcttcg ggctcgagcg gatcatcgcc
     7081 cagcgggccg ccgaggaaaa gcacctggcc agcatctatc tcctgatcga caagcggggc
     7141 gtgccgatcg ccggcaacct gccggcctgg ccgaccgatg tcgagaccgt cagcaaccgc
     7201 ttccgcttct ccctggacct gccgggcagc tcggggccga ggcggttcct cggccgctcg
     7261 gtcgagctcg atcagggctt tctcctcgtc gcccgcgata tcgaggacaa gctccggacc
     7321 cagaccctgc tcgtcaacgc catcgccctg ggcagcggcc tgatgctcgt cttcggcgtc
     7381 atcggcggct tcgtcatgag ccggtggatg ctcacgcgca tcgagacgat caaccgggcg
     7441 accgggcaga tcat
//
LOCUS       DAOTFI010000231         7407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11966_length_7407_cov_6.513330, whole genome shotgun sequence.
ACCESSION   DAOTFI010000231 DAOTFI010000000
VERSION     DAOTFI010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11966_length_7407_cov_6.513330"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="P5558_19295"
     CDS             <1..1473
                     /locus_tag="P5558_19295"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY26521.1"
                     /translation="FPGVLALDDVSLAIGRREVVGLIGENGAGKSTLLKVLNGIYQLD
                     QGEVRVAGKPVQIRHPRDAFDHGIAMVFQEQSVFSTLSVAENIFLAREAEFLRGGLID
                     RRRLNEAARAELAKVHLDVDPRRRCAGLTFAERQMVEIAKALSLDTRIEGDITILLDE
                     PTSMLEQREVDLLFEIVADLKDRASIVFISHRLDEVLEVADRVYVMRDGKVVHEVASR
                     DATIPELHRHMVGRALHHEYYREARQAPGDGAAVLEVEGLTGRHGFRDVSFTLHAGEV
                     LGIAGVVGSGREALARCLAGLEPPAAGTVRIDGRKVALASPSEAVARGIGFIPSERKT
                     EGLVAGLSVAENMTLAALGRFTARGLIRVAEETKAAKSWIERLSIKTPSPATPVGSLS
                     GGNQQKVVLAKWRIAGSRVVILDHPTRGIDVGAKEDVYTLVRDMTDEGLAVILLGDTL
                     EEVIGLSSRILVMRDGRIEAELEAPPGGKPGQVDLIARMV"
     gene            1482..2438
                     /locus_tag="P5558_19300"
     CDS             1482..2438
                     /locus_tag="P5558_19300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26522.1"
                     /translation="MATPLLSRARVQNALPVMALVVLTVIVGTIEPTFLAPANLLVVT
                     ADTMTLFLMASGVTFVIMLGCIDLSIQAVASMTSCIVAAMLAQYGFVAVPVAVLAGAL
                     SGLLSGYVYVVLRIPSFIATLAVSGVMTTVAYIASDTRSVPIPAEARDAWLFWATGRS
                     LGVPHEIVVGLVVLVVLGGVLALTPFGRYVRAIGSQEQAVHASGIAVGRVKILAFALS
                     GTMAGIAGVVMGARLGSGSPTLANEFLLPAIACVIVGGTAITGGVGSIWKTFIGALTI
                     AVVRIGMTFLGVSVFAQQIVFGLILVAAVALTMDRSKVAVVK"
     gene            2474..3319
                     /locus_tag="P5558_19305"
     CDS             2474..3319
                     /locus_tag="P5558_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY26523.1"
                     /translation="MEGIDPGNVLVLRHRPFEVELRKVLPWLAAERPEMFNAYQQTQT
                     NPKVEGAFQKATHIASFIGHQAGKALFIGLYERGGWRPLGYEAYWHVAAYREMKAFGI
                     QGFTGDRDAVLWFDLELTEFYQQWRGRLIVGWPGLERSWWRWAERNIIPVESILEQSA
                     LDAEMPHWDTLTLTWDELKVLPSRWRSALSQWRGIYFIFDESDGKGYVGAAYGSDNIL
                     GRWLNYASTAHGGNKKLRNRDPASFRYSILQRVSPDMEPAEVIGLEGTWKDRLHTRRF
                     GLNEN"
     gene            3450..4619
                     /locus_tag="P5558_19310"
     CDS             3450..4619
                     /locus_tag="P5558_19310"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26524.1"
                     /translation="MKISRLDVYPLSIAYVRNETSSIINRGGVSDVVVRLTTDDGLVG
                     WGECVAPGDTESIVAAARAAEPFLLGRDPWDQEAIFRDYLVAGNWQFQAMTGNFAFAG
                     IDMALWDICGKAVGQPLWRLFGGALRQSVDYFFYLEWGEPDAVAAQGAAGRRLGYSVF
                     YLKVGIDAVREEAMLQALRDAIGPEGRIRIDANQSWTLPEAARLLARWDGLVGLDFAE
                     APVPIDPVEGMIELKGRVAVPLCANEGLWREADAVRIIQSRAADYLCLGPSWVGSLRR
                     FQSVARAAHLQGQQVCKHTHGEHGIAAAASQHLLLTLPNACQGHQQTAQMMRDDILVE
                     RIPIMDGPSWGLIEGPGLGIEIDEAKLQMYHEAYLRDGEYVTWGTGFKPRRENEA"
     gene            4616..5455
                     /locus_tag="P5558_19315"
     CDS             4616..5455
                     /locus_tag="P5558_19315"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRY26525.1"
                     /translation="MSGIEIEPIAGSLGAEIRGVDLTRPLADADAADIRGALGQHSVI
                     FLRGQKITPEQHIAFARSFGPIVVNRFFTPVDGHPEIAEVRKEPEQKRNIGDIWHTDH
                     SYDEKPAMGSVLLAREVPSRGGDTMFASMHLAYEALSDGLKRTLETLRARHSSRHVFG
                     PEAPYHGSDLASRLRNTEAATQDVVHPVVIRHPDTGRKALYVNRSFTTGIEGWNPDES
                     AALLGFLYEHGSRPEFTCRFRWQPGSIAVWDNRSTWHYAVNDYQGERRLMHRITIEGV
                     ALQ"
     gene            complement(5427..6611)
                     /locus_tag="P5558_19320"
     CDS             complement(5427..6611)
                     /locus_tag="P5558_19320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY26526.1"
                     /translation="MHYRWVVLAVLFLARTSMAFQFQSVAALAPLVAADHGASLADIG
                     LLIGLYFAPGIVIAIPGGAIARLLGDRRVLAGGMVLMLAGGLLPALSGSWDALVAGRL
                     IAGIGGVVLNVQLAKMLTDWFLGREIATAMAVLINAWPIGIATALLVLPAMADPGNLA
                     PAWLTVLALVALGFALFLLAWRAPPAASANEAGAGRAVALPLVALSLAGAVWALYNGA
                     LVMVFGFGPTLLSERGWPLAEASSTTSIVLWLMVVSVPLGGLLADRSGRRDTVLALGL
                     AGFALLLVAAALAPLRAVPAIFVGLGLVSGISGGPILSLPSLVLRPETRALGMGVFFT
                     FSYLALMVSPTLGGWLAERTGDAGTAFVLGAAMLVLGLAALGLFRRDVGRAATAAPRP
                     RS"
     gene            6728..6985
                     /locus_tag="P5558_19325"
     CDS             6728..6985
                     /locus_tag="P5558_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HRY26527.1"
                     /translation="MHTTKLRKVGGSTMLAVPPALLDLLELRAGATVGVTIDDGRLIV
                     EPRPRRRYTLDELLAACDAAAEPAGDEREWLETGPVGNELL"
     gene            6985..7317
                     /locus_tag="P5558_19330"
     CDS             6985..7317
                     /locus_tag="P5558_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRY26528.1"
                     /translation="MERGDIYLVALDPSLGHEQQGRRPVLVVSPASFNKVTRVPVVLP
                     ITSGGAFARNAGFAVSLSGAGTQTTGVVRCDQPRALDLQARGGRRLERVPDAIVEEVL
                     ARLAPIFE"
BASE COUNT         1183 a         2434 c         2658 g         1132 t
ORIGIN      
        1 tttcccggcg tgctggcgct cgacgacgtg tcgctcgcca ttggccggcg cgaggtggtc
       61 gggctgatcg gcgagaacgg cgcgggcaag tcgaccctgc tcaaggtgct gaacggcatc
      121 taccagctcg accagggcga ggtccgggtc gccggcaagc cggtgcagat caggcacccg
      181 cgagacgcct tcgaccacgg catcgccatg gtcttccagg agcagtcggt cttctcgacg
      241 ttgtcggtgg cggagaacat cttcctcgcc cgcgaggccg agttcctgcg aggcgggttg
      301 atcgaccggc ggcggctcaa cgaggcggcc cgggccgagc tcgccaaggt gcatctcgat
      361 gtcgatccga ggcgccgctg cgccgggctc accttcgccg agcggcagat ggtcgagatc
      421 gccaaggccc tttcgctcga cacacggatc gagggcgaca tcaccatcct gctcgacgag
      481 ccgaccagca tgctcgagca gcgcgaggtc gacctgctct tcgagatcgt cgccgacctc
      541 aaggaccggg cgtccatcgt cttcatctcg caccggctcg acgaggtctt ggaggtcgcg
      601 gaccgggtct atgtgatgcg cgacggcaag gtggtgcacg aggtggcgag ccgcgatgcc
      661 accattcccg agctgcaccg ccacatggtg ggccgtgccc tgcaccacga gtattatcgg
      721 gaggcccggc aggcgccggg cgatggcgcg gcggtgctcg aggtggaagg cctcaccggg
      781 cggcacggct ttcgcgatgt ctcgttcacc ctccatgcgg gcgaggtcct gggcattgcc
      841 ggcgtggtgg gctcggggcg cgaggcgctg gcccgctgcc tcgccgggct cgagccgccg
      901 gcggcgggca cagtgcggat cgacggccgc aaggtggcgc tggccagccc ttccgaggcc
      961 gtggcgcgcg gcatcggctt cattccgagc gagcgcaaga ccgaggggct cgtggccggg
     1021 ctcagcgtcg ccgagaacat gacgctcgcc gccctcgggc ggttcaccgc acgcgggctg
     1081 atccgggtgg ccgaggagac gaaggcggcg aagtcctgga tcgagcggct gagcatcaag
     1141 acaccctcgc cggcgacacc cgtcggcagc ctttccggtg gcaaccagca gaaggtggtg
     1201 ctggcgaagt ggcggatcgc cggctcgcgc gtggtgatcc tcgaccatcc gacgcgcggc
     1261 atcgacgtgg gggcgaagga ggacgtctac acgctggtcc gcgacatgac cgatgagggg
     1321 ctggccgtga tcctgctcgg cgacacgctc gaggaggtga tcggcctctc gtcgcgcatc
     1381 ctcgtcatgc gtgacgggcg gatcgaggcc gagctcgagg ccccgccggg cggcaagccc
     1441 gggcaggtcg acctcattgc gcgcatggtc tgagcgacgc catggccacc cctctcctct
     1501 cccgcgcccg cgtgcagaac gccctgccgg tcatggcact cgtcgtgctg accgtgatcg
     1561 tcggaacgat cgagccgacc ttcctcgcgc cggccaatct cctggtggtc accgccgaca
     1621 ccatgacgct cttcctcatg gcgtccgggg tcacgttcgt catcatgctc ggctgcatcg
     1681 acctttcgat ccaggccgtg gcgtcgatga cgagctgcat cgtggcggcg atgctggcgc
     1741 aatacggctt cgtcgcggtg ccggtggcgg tgctcgccgg cgccctctcg gggctgctct
     1801 cgggctatgt ctacgtggtg ctgcgcatcc cctccttcat cgccacgctg gcggtgagcg
     1861 gggtgatgac gacggtggcc tacatcgcct ccgacacgcg ctcggtgccg atcccggccg
     1921 aggcccgcga cgcctggctc ttctgggcca ccggccgcag cctcggcgtg ccgcacgaga
     1981 tcgtcgtcgg cctcgtggtg ctggtggtgc tgggcggggt cctcgccctc acccccttcg
     2041 gccgctacgt cagggcgatc ggctcgcagg agcaggcggt gcatgcctcg ggcatcgcgg
     2101 tcggccgggt caagatcctg gccttcgccc tttcgggcac catggccggg atcgccgggg
     2161 tggtcatggg ggcgcggctc ggctcgggct cgccgaccct cgccaacgag ttcctgctgc
     2221 cggccatcgc ctgcgtcatc gtcggcggca cggccatcac cggcggggtc ggcagcatct
     2281 ggaagacgtt catcggggcg ctgaccatcg cggtcgtgcg catcggcatg acgttcctcg
     2341 gcgtttccgt cttcgcccag cagatcgtct tcggcctgat cctcgtcgct gccgtggcgc
     2401 tgaccatgga ccggagcaag gtggcggtgg tgaagtaggg atggctccgg tgcagctaaa
     2461 cgacctcctc cgcatggagg gtatcgaccc cgggaatgtc ctcgttctcc gccatcgccc
     2521 attcgaggtc gagctccgaa aggtgttgcc ctggcttgcc gctgaacgtc cggagatgtt
     2581 caacgcctac cagcaaacgc agacgaaccc gaaggtcgag ggtgcatttc aaaaagcgac
     2641 gcacatcgca tcgttcattg gccatcaggc cgggaaggcg ctgtttatcg ggctgtacga
     2701 gcgcggcggc tggcgaccgc taggctatga ggcgtattgg catgtcgccg cctaccggga
     2761 aatgaaggcg tttggcatcc agggcttcac cggcgatcgc gacgccgttc tatggttcga
     2821 tctcgaattg accgaattct atcaacaatg gaggggccga ctgattgtcg gctggcccgg
     2881 ccttgaacgc tcgtggtggc ggtgggctga acgaaacatc attccagtcg agtcgatcct
     2941 cgagcaaagc gccctggatg ccgagatgcc ccactgggac acactgacat tgacctggga
     3001 cgagctgaag gttctgccat cgcgatggag gtcagcactg tcgcagtggc gtggcattta
     3061 cttcatcttc gacgaatcgg acggcaaggg ctacgttggt gcggcttacg gaagcgacaa
     3121 catcctcggt cggtggctga actatgcgtc gaccgctcac ggtggcaaca agaagctccg
     3181 caatcgcgac cctgcaagct tccgctattc gatcttgcag cgggtgtcgc ccgatatgga
     3241 gcccgccgaa gtgatcggac tcgagggaac gtggaaggat cggctccaca cgcggcgatt
     3301 cggcttgaac gagaactagc atggagccga tcaaccttca ccacgggacc cgccgcaagg
     3361 cgccccacaa caaccagaaa ttcacggtcg ggccgccgtg gcgctgacca tggaccggag
     3421 caaggtggcg gtggtgaagt agggatgcga tgaagatcag ccggctcgac gtctacccct
     3481 tgagcatcgc ctacgtgcgc aacgagacga gctcgatcat caaccggggc ggtgtctcgg
     3541 acgtggtggt gaggctcacg accgatgacg gcctcgtcgg ctggggcgag tgcgtggcgc
     3601 cgggcgacac ggagagcatc gtggcggcgg cccgggccgc cgagcccttc cttctgggcc
     3661 gtgatccctg ggaccaggag gcgatctttc gggactacct ggtcgccggc aactggcagt
     3721 tccaggcgat gaccggcaat ttcgcctttg ccgggatcga catggcgctc tgggacatct
     3781 gcggcaaagc ggtgggccag ccgctctggc gactcttcgg cggcgccttg cggcagagcg
     3841 tcgactattt cttctatctg gaatggggcg agcccgacgc cgtggcggcg cagggtgcgg
     3901 cggggcggcg gctcggctat tcggtcttct atctcaaggt cggcatcgac gcggtgcgcg
     3961 aggaggcgat gctgcaggcc cttcgcgacg ccatcggccc ggagggccgg atccgcatcg
     4021 acgccaacca gtcctggacc ctgcccgaag ccgcccggct gctggcgcgc tgggacgggc
     4081 tggtggggct cgacttcgcc gaggcgccgg tgccgatcga cccggtggag ggaatgatcg
     4141 agctgaaggg acgggtggcg gtgccgctct gcgccaatga ggggctctgg cgggaggcgg
     4201 atgcggtgcg gatcatccag agccgcgctg ccgattatct ctgtcttggc ccgtcctggg
     4261 tcggctcgct ccggcgcttc cagtccgtcg cccgggcggc gcatctccag ggccagcagg
     4321 tctgcaagca cacccatggc gagcacggca tcgccgcggc ggcgagccag catctcctgc
     4381 tcacgctgcc caatgcgtgc cagggccacc agcagacggc gcagatgatg cgggacgaca
     4441 tcctggtcga gcggattccg atcatggacg ggccgagctg ggggctgatc gaggggccgg
     4501 ggctcgggat cgagatcgac gaggccaagc tgcaaatgta ccacgaggcc tatctgcgtg
     4561 acggcgagta cgtcacctgg gggacgggct tcaagccacg acgggagaac gaggcatgag
     4621 tggaatcgag atcgagccca ttgccgggtc gctgggtgcc gagatccgcg gcgtggacct
     4681 gacgcggccg ctcgccgacg cggacgcggc cgacatccgg ggcgccctcg gccagcactc
     4741 ggtcatcttc ctgcgcggcc agaagatcac gccggagcag cacatcgcct tcgcccggag
     4801 cttcgggccg atcgtcgtca accgcttctt caccccggtc gacggccacc cggagatcgc
     4861 cgaggtcagg aaggagccgg agcagaagag gaacatcggc gacatctggc acaccgacca
     4921 ttcctatgac gagaagccgg ccatgggctc ggtgctgctc gcccgcgagg tgccgagccg
     4981 cggcggcgac acgatgttcg ccagcatgca cctcgcctac gaggcgctct cggacggcct
     5041 caagcggacg ctcgagaccc tgcgcgcccg gcacagcagc cgccacgtct tcggccccga
     5101 ggcgccctat cacggcagcg acctcgccag ccggctgcgc aacaccgagg ccgcgaccca
     5161 ggacgtcgtg catcccgtgg tgatccgcca cccggatacc ggccgcaagg cgctctacgt
     5221 caaccgcagc ttcaccaccg gcatcgaggg ctggaacccg gacgagagcg cggcgctgct
     5281 gggcttcctc tacgagcacg gctcgaggcc ggagttcacc tgccgctttc gctggcagcc
     5341 gggctcgatc gcggtctggg acaaccgcag cacctggcac tacgccgtca acgactacca
     5401 gggcgagcgc cggctgatgc accggatcac gatcgagggc gtggcgctgc agtagcggct
     5461 ctgccgacgt ctcgccggaa gagcccgagg gcggcgagac cgagcaccag catcgccgcc
     5521 cccaggacga aggcggtgcc cgcgtccccc gtgcgttcgg cgagccagcc gccgagggtc
     5581 ggcgagacca tgagcgcgag gtaggagaag gtgaagaaga ctcccatgcc gagtgctcgg
     5641 gtctccgggc ggaggacgag cgacggcagg ctcaggatcg gcccgccgga gatgcccgag
     5701 acgaggccga ggccgacgaa gatcgccggc accgcccgga gcggggcgag cgcggcggcg
     5761 accaggagaa gggcgaagcc ggcgaggccg agggcaagga cggtgtcgcg gcgaccgctg
     5821 cgatcggcga ggaggccgcc gagcggcacc gagaccacca tcagccagag gacgatgctg
     5881 gtggtcgagc tcgcttccgc aagtggccag ccgcgctcgc tgagcagcgt cggcccgaag
     5941 ccgaagacca tgacgagggc accgttgtag agcgcccaga cggccccggc gagcgagagg
     6001 gcgacgagcg gcaaggcaac ggcgcgaccg gcgccggcct cgttcgccga tgctgcgggc
     6061 ggtgcccgcc aggcgaggag gaagagggcg aagccgagag cgaccaatgc caggaccgtg
     6121 agccaggcgg gtgcgaggtt gccggggtcg gccatggccg gcagcaccag gagggccgtg
     6181 gcgatgccga tgggccaggc attgatgagc accgccatgg cggtcgcgat ctcgcggcca
     6241 aggaaccagt cggtcagcat cttcgcgagc tgcacgttga ggacgacccc gccgatgccg
     6301 gcgatcaggc ggccggcgac gagcgcgtcc cagcttcccg acagggccgg gaggaggccg
     6361 cctgcgagca tcaggaccat gccgccggcg agcacccggc ggtcgcccag cagccgcgca
     6421 atggcgccgc ccgggatcgc gatgacgatg ccgggtgcga aatagagccc gatcagcagc
     6481 ccgatatcgg cgaggctggc gccatggtcc gcggccacca gcggtgcgag ggccgccacc
     6541 gactggaact ggaacgccat ggacgtccgg gcgaggaaga ggacggcaag gacgacccag
     6601 cggtagtgca tcgatggcgc tcttccgggc ggtagcggcg ggacgcggcg acgcccatag
     6661 cagagttcat ggcggatggc gtcgccagag gtttgtgtta tactccagta taattctgga
     6721 gtgacacatg cacacgacga agctgcgcaa ggtcgggggc tcgacgatgc tggctgtgcc
     6781 tcctgccctg ctcgatcttt tggagctgcg cgccggtgcg acggtcggcg tcacgatcga
     6841 cgacgggcgc ctgatcgtcg agccccggcc gcgccggcgc tacacgctcg acgagttgct
     6901 ggcggcgtgc gacgcggcgg ccgagccggc cggcgacgag cgtgaatggc tggagacggg
     6961 gccggtcggc aacgagcttc tctgatggag cgcggcgaca tctatctggt ggcgctcgac
     7021 ccgagcctcg gccacgagca gcaggggcgc cggccggtgc tggtcgtctc acccgcttcc
     7081 ttcaacaagg tcacgcgcgt gccggtcgtc ctgccgatca ccagcggcgg tgccttcgcc
     7141 cgcaatgccg gcttcgccgt ctcgctttcc ggcgcgggga cccagacgac gggcgtggtg
     7201 cgctgcgacc agcccagggc gctcgacctg caggcgcgtg gcggtcgccg gctcgagcgg
     7261 gtgcccgatg ccatcgtcga ggaggtgctc gcccggctgg cgccgatctt cgagtaaggg
     7321 ccagccgtgc tcagaacttg ccgtagagga gataggcctc gcgcgggtcg acgccttcga
     7381 ggatggccgt cctgaccttg ttctcgg
//
LOCUS       DAOTFI010000232         7403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_11979_length_7403_cov_6.863092, whole genome shotgun sequence.
ACCESSION   DAOTFI010000232 DAOTFI010000000
VERSION     DAOTFI010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11979_length_7403_cov_6.863092"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..517)
                     /locus_tag="P5558_19335"
     CDS             complement(191..517)
                     /locus_tag="P5558_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26529.1"
                     /translation="MTEVRQERALYCYASGHEGAWEAICLDLDIAVQGRSFEEVSSLL
                     REAVSLYLETVGELPEDERSAFLNRSVPFRTRLRFAIEAFWSALRARGNGELKHQFTM
                     AMPTPA"
     gene            699..2081
                     /locus_tag="P5558_19340"
     CDS             699..2081
                     /locus_tag="P5558_19340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY26530.1"
                     /translation="MTLRFEQILAEGIAQTSYLVGDDAEGTAAVVDPRPDAEIYLDLA
                     RHFGLTITHVLETHIHADFMSGARELVDRLGGSAELCVSEEGGAEYDFPHRRLRPGER
                     LPFGAVELEVVATPGHTPEHVSFLLRERGREQPWGILSGDSFFVDSVGRPDLLGDEQT
                     EELTRALFRTLHEVYRPLPDGTIVYPCHGAGSECGPAIGDRTSTTIGFERQHNTYFAI
                     DDYEEFRQAMQADAPPVPTHYPRLKKVNAAGPAVLGNLPRVPALGPGAFAAAARGGRL
                     VDTRDMLAFGGGHIAGAINIGGRPDLSVWAGWLLDPDQPLFIVLDDDTSLARIVTLLW
                     RVGFTRFGGYLAGGMAAWQEAGQPLRHLPQLTVHELCENPDLLPLDVRKEDEWEGGHV
                     PGARHLFLGELPDRMQELDRDQPIATYCASGFRASIAASLLAASGFDVRNVPGSWKAW
                     RAAGFDVAKE"
     gene            2093..2587
                     /locus_tag="P5558_19345"
     CDS             2093..2587
                     /locus_tag="P5558_19345"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HRY26531.1"
                     /translation="MDEVIRLNADYAVASSEPARRTFDRIAAEGFRSVVSFQTREEEQ
                     KLPIEDEREAAAQAGLAFLHQPVSGDSLDDETVDRFRDALGGLPKPVLLHCSSGKRAG
                     AMTMMHLASEMGLSGDEALEKAASMGFECDNPKLEAFVRDYVDRHQRRSSATPSAAAT
                     ADRR"
     gene            complement(2550..3404)
                     /locus_tag="P5558_19350"
     CDS             complement(2550..3404)
                     /locus_tag="P5558_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRY26532.1"
                     /translation="MSSIACNFRIAALAAMSIFGVASTALADAQVERGRYLVEVIGAC
                     GNCHTPMGPEGPDTSRHLAGGMVIDMDVFRAVPANITPDPETGIGAWSDVEIARAIRE
                     GVRPDGSLIGPPMPYPLYHGIADDDLMAIVAYLRTVPPVVNETEPSVYNIPLPPAWTD
                     PIASVPAPDPSDQVAYGAYLAGPVGHCVECHTPMGEHGHPDFEHRLGAGGFEFPGPWG
                     VSVAANITPTGLGDWSDAEIARAIREGVSRDDRPLMPPMAFAYYKGISEPDMAAIIAY
                     LRSLPPME"
     gene            complement(3601..3855)
                     /locus_tag="P5558_19355"
     CDS             complement(3601..3855)
                     /locus_tag="P5558_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26533.1"
                     /translation="MTDNPVVYDAETDTLLVTLRPWPGTEGDIGRVGGTDAGDDLVIH
                     YAPDGEPWAWEIEHASTHPELLAEALAAVRRAVADSHAAA"
     gene            complement(4103..4849)
                     /locus_tag="P5558_19360"
     CDS             complement(4103..4849)
                     /locus_tag="P5558_19360"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRY26534.1"
                     /translation="MDERSPSRIGILETGRPPEALNRHGSYPEMFERLLGPDFACTSY
                     PVLDDVFPERVDEMEGWLVTGSRFGAYDPEPWIRRLEGFLRDALAARVPVVGICFGHQ
                     VLASALGARVEKAAAGWGVGPHRYDILERPGWMAEAGDAFTLNAMHQDQVLDMPPGAR
                     LVASSDFCPIAALAYGDHAISFQAHPEYDNAFERELIEMRRGSRIPEERADPALAALR
                     DGGGDAGVAPDGPRVAEWIKRFFRASAAHQ"
     gene            complement(4855..5913)
                     /locus_tag="P5558_19365"
     CDS             complement(4855..5913)
                     /locus_tag="P5558_19365"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HRY26535.1"
                     /translation="MAGSRIAVIAGDGIGKEVVPEGLRVLDKVAGRFDLAFDFDELDW
                     SSDRYARTGRFMPEDGLDSIANHDAIFLGAVGWPTVPDHVSLWGLLIPIRRRFDQYVN
                     LRPVRLFEGVKCPLADRKPGDIDFLIVRENVEGEYSEIGGRLYAGLPQEMAVQESIFT
                     RHGVDRIVRYAFELAMTRPKRHLTSATKSNGIIHTMPYWDERFAAVAEDYPEIRTDKF
                     HIDILTAHFVRHPDWFDVVVGSNLFGDILSDLGPAVTGTIGIAPSANIDPERRFPSMF
                     EPVHGSAPDIAGQGIANPIGQIWSAAMMLEFMGHQAAADAIVAAIERVLLRPDLLTPD
                     MGGKATTEALGKAIAAEI"
     gene            6036..7262
                     /locus_tag="P5558_19370"
     CDS             6036..7262
                     /locus_tag="P5558_19370"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY26536.1"
                     /translation="MHNPFATIAARDPRRLWLAGLGVNMMRWLELLAYGVLALQLTGS
                     ALWVATMTIARLLPLLTGAVVTAAISGHSRKTVVTTVVALLVVTDIVLLGLALTGRLA
                     LWQLLIAAVMGGIAWTIETPVRRTMLADAVGLERVSAAMGLEVVGVQLTRIMGPAIGG
                     ILLDMGGIASVFAMGIVVHGLCTWMMLSQPPDRPTTAPANLAGLARNLAEGFTYVRRH
                     RLLTGAVMLTVVFNFFGFPYVALAPVIGERVLHLGPTGIGLLLASDAVGALIAAFILT
                     ERSRPELSGRIYSLGASLYLMATLVFGLTSSIGVAFTVLFIGGFGMAGFNAMQISLPL
                     QATPAPIRVRVLGIVTFAIGAAPFGFLHAGLLAEWLGAVNAQRLIAAEGLAAAALVLW
                     FWPELLKREPPRPLPD"
BASE COUNT         1120 a         2729 c         2426 g         1128 t
ORIGIN      
        1 aagctgctga ccgcccacgg cttcgagctg atccgccagc agggcagcca tcgccacttt
       61 cagggattcg tcgacggcaa gcgatgcatc gtgaccctgg cctgaccacg cgcgcaacga
      121 cgacatattg cccaagacgc tggcctcgat catccggcag tccggcctgc cgaagcgcct
      181 cttcgaatga tcaggcggga gtcggcatcg ccatcgtgaa ctggtgcttc agctcgccgt
      241 tgccccgtgc ccgaagagcc gaccagaagg cttcgatggc gaagcgcagg cgcgtccgga
      301 acgggaccga cctgttgagg aacgcggacc gttcgtcctc gggcagctcg cccaccgtct
      361 ccagatacaa agaaaccgcc tcccgcagca gggacgacac ctcctcgaag gatcgaccct
      421 gcacggcgat gtcgaggtcg aggcaaatgg cctcccaggc accctcgtgg cccgacgcat
      481 agcaatacag cgctcgctcc tgccgcacct cggtcaacgc atcgtcctct cgcacgagcc
      541 tatcgacacg gtaaagtaag cgacctgcag caccttgtca gcccctgcct gtcggcgcga
      601 gttcatccgc tgatccaggc gaagccgcac cagccgacca tcgccggaac ccgaagccgc
      661 cggcgccgtt ggagcggtga accagtggag gaaggccgat gaccctgcgc ttcgagcaga
      721 tcctcgccga gggcattgcc cagacgtcct acctcgtcgg tgacgacgcc gagggcacgg
      781 cggccgtggt cgatccgcgg ccggacgcgg agatctatct cgacctggca cgccacttcg
      841 gcctcaccat cacccacgtc ctcgagaccc acatccacgc ggacttcatg agcggcgcgc
      901 gcgagctggt cgatcgcctc ggcggcagcg ccgagctctg cgtcagcgag gagggcggcg
      961 ccgagtacga cttcccgcac cgccgccttc gccccggcga gcgcctgccc ttcggtgcgg
     1021 tcgagctgga ggtggtcgcc acgccgggac acacgccgga gcacgtctcc ttcctcctgc
     1081 gcgagcgcgg ccgcgagcag ccctggggca tcctcagcgg cgattccttc ttcgtcgatt
     1141 cggtcggccg cccggatctc ctcggtgacg agcagaccga ggagctgacc cgcgccctct
     1201 tccgcacgct gcacgaggtc taccggccgc tgcccgacgg cacgatcgtc tatccctgcc
     1261 acggcgccgg ctccgaatgc ggccccgcca tcggcgatcg cacctccacc accatcggct
     1321 tcgagcggca gcacaacacc tacttcgcca tcgacgatta cgaggagttc cgccaggcga
     1381 tgcaggcgga cgcgccgccg gtgccgaccc actatccccg cctgaaaaag gtcaacgccg
     1441 ccggccccgc cgtgctcggc aacctgccgc gcgtgccggc gctcggcccc ggcgcgttcg
     1501 ctgccgccgc ccgcggcggc cggctcgtcg acacccggga catgctcgcc ttcggcggcg
     1561 gccatatcgc cggcgccatc aacatcggcg gccgccccga cctctcggtc tgggccggct
     1621 ggctcctcga cccggaccag cccctcttca tcgtgctcga cgacgacacc agcctcgccc
     1681 ggatcgtcac cctgctctgg cgcgtcggct tcacccgctt cggcggctat ctcgccggcg
     1741 gcatggcggc atggcaggag gcgggccagc cgctccggca tctcccgcag ctcaccgtcc
     1801 acgagctctg cgagaacccg gacctgctgc cgctcgacgt ccgcaaggag gacgagtggg
     1861 agggcgggca tgtgcccggc gcccggcatc tctttctcgg cgagctgccc gaccggatgc
     1921 aggagctcga tcgggaccag cctatcgcca cctactgcgc cagcggcttc cgggccagca
     1981 ttgccgccag ccttctggca gcttcgggct tcgacgtccg caatgtcccg gggagctgga
     2041 aggcgtggcg cgcagcgggc ttcgatgtcg cgaaggaatg aggagaaagg caatggacga
     2101 ggtcattcga ctgaacgcgg actatgccgt cgccagctcg gagccggcac ggcggacgtt
     2161 cgaccgcatc gcggccgaag ggttccgttc ggtcgtgagc ttccagaccc gggaggagga
     2221 gcagaagctg ccgatcgagg acgaacgtga ggcggccgcg caggcgggcc tcgccttcct
     2281 gcatcagccg gtctcgggcg attccctcga cgacgagacc gtcgaccggt tccgcgacgc
     2341 cctcggcggc ttgccgaagc ccgtcctctt gcattgcagc agcggcaagc gcgcaggggc
     2401 catgaccatg atgcacctgg cgagcgagat gggactctcc ggagacgagg cgctggagaa
     2461 ggccgcatcc atgggcttcg aatgcgacaa cccgaagctg gaggccttcg tcagggacta
     2521 cgtcgatcgc caccagcgcc ggtcatccgc tactccatcg gcggcagcga ccgcagatag
     2581 gcgatgatcg ccgccatgtc gggctcgctg atgcccttgt agtaggcgaa ggccatgggc
     2641 ggcatgagcg gccggtcgtc gcggctgaca ccctcgcgga tggcccgcgc gatctcggcg
     2701 tcggaccagt cgccgagccc cgtgggcgtg atgttggcgg cgaccgagac gccccagggg
     2761 ccggggaact cgaagccacc ggcacccagc cggtgctcga agtccggatg gccgtgctcg
     2821 cccatcggcg tgtggcactc gacgcagtgg ccgaccggcc cggcgagata ggcaccgtag
     2881 gccacttggt cggacgggtc gggcgccggc accgaggcga tcgggtcggt ccaggcgggc
     2941 ggcaggggga tgttgtagac cgagggctcg gtctcgttga ccaccggcgg cacggtcctg
     3001 agataggcga cgatcgccat caggtcgtcg tcggcgatgc cgtgatagag cggatagggc
     3061 atgggcgggc cgatcaggct gccgtcgggg cgcacaccct cgcggatcgc ccgcgcgatc
     3121 tccacgtcgc tccaggcgcc gatgccggtc tcgggatcgg gtgtaatgtt cgccggaacg
     3181 gcccggaaga catccatgtc gatcaccatg ccaccggcca gatggcggct cgtgtccgga
     3241 ccttccggcc ccatcggcgt atggcagttg ccgcaagcgc cgatgacctc gaccagatag
     3301 cggccgcgct cgacctgggc atcggccaag gcggtcgacg cgacgccgaa aatcgacatc
     3361 gcagccaggg ccgcaatcct gaaattgcac gcaatcgatg acatcgctgg tcattctccg
     3421 gaccgccccg gaccggctgg ctacggccgg gggcttgttg ttgctccagg cgccgcccgg
     3481 cctccccggc gcgaccccgc ggcaagctgc gtcggctggc cgcaggtcgt caagcccccg
     3541 cgtcaggccg cccggccgac gccatgaagg accgccggcg tttccagtgg tgtggttcga
     3601 tcacgctgcc gcgtggctgt cggccacagc ccggcgcacc gccgccagcg cctccgccag
     3661 gagctccgga tgggtcgagg catgctcgat ctcccaggcc cagggctcgc catccggcgc
     3721 gtagtggatc accagatcat caccggcatc cgtgccaccc acgcggccga tatcgccctc
     3781 cgttccgggc catggccgca acgtcaccag gagtgtgtcg gtctcggcat catagacgac
     3841 gggattgtcg gtcatggtac gaagtccctg tcgatgaagg cgttgtgcac ggtctccccg
     3901 tccgccagga cgatcactct tatccaccgg ttcaacgttg gagaccaccc ccagagccgc
     3961 atgcgcccat ctggctgctc caaggtatga tgtggtgcat cgacgatcga ggcgatttca
     4021 gccagcggaa tgacccgcct gtccggtcga agtcgcgtgg cctcgaaata gccggttcgc
     4081 cagaagggcg gcggcgccag cctcactgat gcgcggccga cgccctgaag aaccgcttga
     4141 tccactccgc cacgcgtggg ccgtctggcg ccacaccggc gtcgccgcca ccgtcccgca
     4201 aggccgccag cgccggatcg gcgcgctcct ccgggatgcg gctgccgcga cgcatctcga
     4261 tcagctcgcg ctcgaaggcg ttgtcgtact cgggatgcgc ctgaaaggag atggcgtggt
     4321 cgccgtaggc gagcgccgcg atcgggcaga aatccgagct cgcgaccagc cgcgcccccg
     4381 gcggcatgtc gagaacctgg tcctggtgca tggcgttcag ggtgaaggcg tccccggctt
     4441 ccgccatcca gcccggccgc tccaggatgt cgtagcggtg cggcccgaca ccccaaccgg
     4501 ctgccgcctt ctcgacccgg gcacccaagg cgctcgccag cacctgatgg ccgaagcaga
     4561 tgccgacgac gggcactcgg gcggcgaggg cgtcccggag gaacccctcg agccgcctga
     4621 tccagggctc ggggtcgtag gcgccgaagc gcgagccggt gaccagccag ccctccatct
     4681 cgtcgacccg ctccgggaag acatcgtcca ggacgggata ggaggtgcag gcgaaatccg
     4741 gcccgagcag ccgctcgaac atctccggat agctgccgtg ccggttcagc gcctcgggcg
     4801 gccgcccggt ctcgagaatg ccgatcctcg agggtgatct ttcgtccatg ccgctcagat
     4861 ctccgccgcg atcgccttgc cgagcgcctc ggtcgtcgcc ttgccgccca tgtccggggt
     4921 gagcaaatcg gggcgcagca gcacgcgctc gatcgcggcg acgatggcat ccgccgccgc
     4981 ctggtggccc atgaactcga gcatcatcgc ggccgaccag atctggccga tcgggttggc
     5041 gatgccctgg ccggcgatgt ccggtgcgga gccgtgcacc ggctcgaaca tcgaggggaa
     5101 ccgccgctcg gggtcgatat tggccgaagg tgcgatgccg atcgtgccgg tcacggcggg
     5161 cccgaggtcg gagaggatgt cgccgaagag gttggagccg accaccacgt cgaaccagtc
     5221 cggatggcgg acgaaatgcg ctgtcaggat gtcgatgtgg aacttgtcgg tccggatctc
     5281 cggatagtcc tcggccacgg cggcgaagcg ctcgtcccag tagggcatgg tgtggatgat
     5341 gccgttcgac ttggtcgccg aggtcagatg ccgcttcggc cgggtcatcg ccagctcgaa
     5401 ggcgtagcgg acgatgcggt cgacgccgtg gcgggtgaag atgctctcct gcaccgccat
     5461 ctcctgcggc agcccggcat agagccgccc gccgatctcc gagtactcgc cctcgacgtt
     5521 ctcgcgcacg atgaggaagt cgatgtcgcc gggcttccgg tcggcgagcg ggcacttcac
     5581 cccctcgaag agccgcaccg gccgcaggtt gacatactgg tcgaagcgcc ggcggatcgg
     5641 gatgagcagg ccccagagcg agacatggtc cggcacggtt ggccagccca ccgcccccag
     5701 gaagatggcg tcgtggttcg cgatgctgtc gagcccatcc tccggcatga agcggccggt
     5761 ccgggcgtag cggtccgagc tccagtccag ctcgtcgaag tcgaaggcga gatcgaagcg
     5821 gcccgcgacc ttgtcgagca cgcgcaggcc ctccggcacc acctccttgc cgatcccgtc
     5881 gcccgcgatc accgcgatcc tgctgcccgc catcctcgcc tctcccatcc tcgcaccaac
     5941 gccgccggcc cggccttccg ctcgccgacc gcccgtctat agtccgcccg ccggcgaacg
     6001 acaaagggcg gcatcaggag ccgatcagca gcgtcatgca caaccccttc gccacgatcg
     6061 ccgcccgcga tccccgccgc ctctggctgg ccgggctcgg cgtcaacatg atgcgctggc
     6121 tcgagcttct ggcctacggc gtgctggcgc tgcagctcac gggctccgcc ttgtgggtcg
     6181 ccaccatgac cattgcccgg ctcctgccgc tgctcaccgg ggccgtcgtc accgcggcca
     6241 tctccggtca ttcgcgcaag accgtggtga ccaccgtcgt cgcgctgctc gtggtcaccg
     6301 acatcgtgct cctcggcctc gccctgaccg gccggctcgc cctctggcag ctcctgatcg
     6361 ccgccgtcat gggcggcatc gcctggacca tcgagacccc ggtccgccgc accatgctcg
     6421 ccgacgcggt cggcctcgag cgggtctcgg cggcgatggg cctcgaggtg gtgggcgtcc
     6481 agctcacccg catcatgggc ccggcgatcg gcggcatcct cctcgacatg ggcggcatcg
     6541 ccagcgtctt cgccatgggc atcgtcgtcc acggcctctg cacctggatg atgctgagcc
     6601 agccgcccga ccggccgacc acggcacccg cgaacctcgc cgggctcgcc cgcaacctcg
     6661 ccgagggctt cacctatgtg cgccggcacc gcctcctgac cggggcggtc atgctcaccg
     6721 tggtcttcaa cttcttcggc tttccctatg tcgccctcgc cccggtgatc ggcgagcggg
     6781 tgctccacct cggccccacc gggatcggcc tgctcctcgc cagcgacgcc gtgggggcgc
     6841 tgatcgcggc cttcatcctg accgagcgga gccggcccga gctctcgggg cggatctaca
     6901 gcctcggcgc ctcgctctat ctcatggcca ccctcgtctt cggcctcacc agctcgatcg
     6961 gcgtggcctt caccgtgctc ttcatcggcg gcttcggcat ggccggcttc aatgccatgc
     7021 agatcagcct gccgctccag gccaccccgg cccccatccg cgtgcgcgtc ctcggcatcg
     7081 tcaccttcgc catcggcgcc gcccccttcg gcttcctcca cgccggcctg ctcgccgaat
     7141 ggctgggcgc cgtcaacgcc cagcgcctga tcgccgccga gggactcgcc gccgccgccc
     7201 tggtcctctg gttctggccc gaattgctga agcgcgagcc gccgcgaccc ctgccggact
     7261 gagcacgacc ggctacggct cgatccggcg cagcacggcg atgacgatga cggccagcac
     7321 ggtcgccatg gcggcgaggc cgagcagccc ctgcccggcg gcgatgccga tcgccccggc
     7381 catccagagc ccgccgccgg tgg
//
LOCUS       DAOTFI010000233         7281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12227_length_7281_cov_6.810960, whole genome shotgun sequence.
ACCESSION   DAOTFI010000233 DAOTFI010000000
VERSION     DAOTFI010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12227_length_7281_cov_6.810960"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="P5558_19375"
     CDS             <1..190
                     /locus_tag="P5558_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26537.1"
                     /translation="VRLLQLADEAMGAASFALSHPAERVRRDLQLYLRQADPDGMGQR
                     AMERILDDAGERSRWGIG"
     gene            187..927
                     /locus_tag="P5558_19380"
     CDS             187..927
                     /locus_tag="P5558_19380"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRY26538.1"
                     /translation="MSPDGPFPLLAAWRDQPAGTAADLLGGVKSLCIVAPHPDDETLG
                     CGLLLLAAAERGLPVTIVCMTDGSRSHPGSATWPAHRLAAERRLELTRAVRCLAPAAK
                     VVWLGYPDTGLPTDGPALVAARGRLARHLPRRHGALILTTWPHDPHGDHASTARLVQV
                     ARGPDHPARLFHYPIWGRFLEHAPAPGFDRAFLVAGDTAARARKRRALACHRTQMSSL
                     IGDDPEGFVMPEWMQEHFVEHPEIYLAA"
     gene            917..1492
                     /locus_tag="P5558_19385"
     CDS             917..1492
                     /locus_tag="P5558_19385"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRY26539.1"
                     /translation="MPLERTTRHLCRLYRQSPDPWGHATRAYERAKFARTIAALDGRR
                     FRQAVEIGCGIGVLTALLARRCEALLGIDCVASAVASARLRLAGQSHVRLGVGVAPDD
                     LPDLSPDLIVLSEVLYFMTPAEIIRLAAWCDAHAAPAARIVIVSWKGPTGEALSGPAS
                     AALLCRPLVGWSHRSLDGDGYRIDRLDRLDG"
     gene            complement(1498..2775)
                     /locus_tag="P5558_19390"
     CDS             complement(1498..2775)
                     /locus_tag="P5558_19390"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY26540.1"
                     /translation="MLDKKMATLAAAVVLAATGAAAQDKTELNYSGWINLYPHHAAAV
                     GAIETGFEEKFPDVDWILNDVPFDQALKQATVASLAGNAPDNIHLIAGWVPALNDIGA
                     LEPLNDYFTAEEWAQIPQSSLDSVTFDGQIMAMPWVPGPIIMFYNRNLMEQAGLDPDA
                     PPQTWPELLEAAKAICALPDVDGAPIYGVALRTARNPNSAQWTIPIIYGQGGDIVDAD
                     GNVSFDTPEAAAAYEWVRELTDSGCAPSGFTIDETRNTMAAGRAGFIFEGPWGRGLFN
                     NLSGGKLTTAADGDIWAAPMPMDPSGKRRTIGNPHEITISAGSEHKDLAAEFIRHVIF
                     DPEITTAYFEASSQLSTGSMPLLTSGVMGEDAYTQVFVQALPDTNDNPIKSPKFYAVM
                     DDLVVALQSIIQGGDIQGELASAQRKAERTMSR"
     gene            complement(2803..3699)
                     /locus_tag="P5558_19395"
     CDS             complement(2803..3699)
                     /locus_tag="P5558_19395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY26541.1"
                     /translation="MNAEALRTGSKPLPRRRSEVVYRLSRLATSISLHALVVASLAIL
                     LLPIVWMLSTSFKPLADVFSYPPQFIPRNPTVESYTTQFTGLLGRYFLNSVIVGLLSA
                     ILATGAGALAAYGLSRYRLPGRNAILMFFMASLAFPIPLLMISMYLMFVQLGLLNTYI
                     GLVIGHTVITLPVCVWLLKNFFDQLPVEVEEAAFVDGASRAYTLFRIVLPMARPALGA
                     SAIFVFVTSWNELLFGLTFVSSTSMRPLPAGISMSFLTEFEGAWSEMMALSVMVSLPI
                     FLLFTVFQRTFMSGVTAGAVKG"
     gene            complement(3696..4580)
                     /locus_tag="P5558_19400"
     CDS             complement(3696..4580)
                     /locus_tag="P5558_19400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY26542.1"
                     /translation="MGFSPRQAPVFLAAIFAPSILLVGLFIVYPVVNSVGLAFTDASP
                     LSSGRSFVGLENFVYLLEDGEFWEVLWNTVFIVGAATIIATACGYGLATLLISGIRAA
                     AIFRAAIFQVWVVPWIVIAILWGWLFSQDYGLVNYLLMQVGLIETPVKWMFDQKASQW
                     AIISGYAWRAIPFIMVICLAALQGIPSELVESAAIDGASFLQRQRYVILPLMQNIILV
                     AALLQAVRFFQEMTMIFVLTQGGPVNATMVLSLYTYKTAFEDWDFGLASAIGTVWLIL
                     LMAFALLYVRVALRRNLA"
     gene            4749..6203
                     /locus_tag="P5558_19405"
     CDS             4749..6203
                     /locus_tag="P5558_19405"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY26543.1"
                     /translation="MASARKPNVILIVADDLGYGDIGAFNEGRVATPNLDRLVSEGVT
                     CRQHYAASPICAPSRAALLTGRYNHRTGAITQHEIHGLDRIALREVTLADRFRHAGYA
                     TGLVGKWHNGTFDRRYEPNARGFQEFVGFCGGWVDYYDYTLRVDDGIRRSDGRYMTDV
                     LTEEALGFVERHKREPFFLHLAYSAPHSPFQAPAGMAEPHLEKGLNRIVATTYAMIER
                     MDEGIGRLLDRLDAEGLAEQTIVLFTSDNGPAFFNPPYMLEPGEDTFNERFNAGLRGS
                     KGWIYEGGIRVPMLLRYSERVPAGSTNDDLIQFVDWVPTLMRLAGLAPMDAPPLDGSD
                     VSACLMGEPADLPDQQFWQWNFYMPCIGTNAAMRDGSWKLIRPMMKGTRFFSPELYAT
                     PDDAALAKAFVAADIAHKRDPSSIRELVPVPRIRLPAPEPLELYDLASDPGEQHDLAA
                     QHPDRVARMQARLEDWFDRVEADRRTTAEAAAVA"
     gene            complement(6211..7020)
                     /locus_tag="P5558_19410"
     CDS             complement(6211..7020)
                     /locus_tag="P5558_19410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY26544.1"
                     /translation="MSDSEFAGKVAIVTGATRGIGKATAWRLAKSGASVAICSSNEKD
                     VAATLAEAEAAGLSMAGMALDLAESVSAERLTKLAVDRFGGLDILVANAGVGTFGGAV
                     EVAEEDWRRVMAINLDAVMLGAKHGIPEMKKRGGGAIVVVASVHSFATLGERLAYVTT
                     KTALLGMTRGMALNHGPDGIRVNAVCPGPIDTPLLRKSWETMFPDRGGDAVLTEQAGR
                     LPLGRLGQPDDIAEAIAFLAGPRSAWITGADLKVDGGLLAMLAMTPPPPRG"
     gene            complement(7017..>7281)
                     /locus_tag="P5558_19415"
     CDS             complement(7017..>7281)
                     /locus_tag="P5558_19415"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRY26545.1"
                     /translation="DRHAPDAAKPCCVPSDPHGGTREGSIDPFAPHLQIPRKVVKGGS
                     FLCTDQYCLRYRPAARQGQAIDTAIRHLGFRCVVPAVDGDLAR"
BASE COUNT         1187 a         2460 c         2511 g         1123 t
ORIGIN      
        1 cgtccgcctg ctccagctcg ccgacgaggc gatgggtgcg gcctccttcg cgctctcgca
       61 cccggccgag cgggtccgcc gcgacctcca gctctatctg cgccaggccg atcccgacgg
      121 catggggcag cgcgccatgg agcgcatcct cgacgatgcc ggggagcgca gccgctgggg
      181 gatcggatga gcccggatgg ccccttcccg ctcctcgccg catggcgcga ccagccggcc
      241 ggcaccgctg ccgacctgct gggtggggtg aagtcgcttt gcatcgtggc cccccacccc
      301 gacgacgaaa ccctcggctg tggcctcctg ctgctggcgg cggcagagcg gggcctgccc
      361 gtcaccatcg tctgcatgac cgatggcagc cggtcccatc cgggctccgc cacctggcca
      421 gcccaccgac tcgccgccga gcgacgcctg gagctgaccc gggcggtccg ctgcctggcg
      481 ccggccgcca aggtggtctg gctcggctac cccgataccg ggctgccgac cgacggcccg
      541 gctctcgtcg cggcccgcgg ccgcctggcc cggcatcttc cccgtcggca cggcgcgctc
      601 atcctgacca cctggccgca cgacccgcat ggcgatcatg cgagcacggc ccggctggtc
      661 caggtcgcac gcggccccga ccacccggcg cggctcttcc actatccgat ctgggggcga
      721 tttctcgagc acgcccccgc accgggattc gaccgggcat tcctcgtcgc cggcgacacc
      781 gccgccaggg ctcgcaagcg ccgcgccctg gcctgtcatc ggacccagat gtcgagcctc
      841 atcggggacg accccgaggg cttcgtgatg ccggagtgga tgcaggagca tttcgtcgag
      901 catcccgaga tctatcttgc cgcttgagcg aaccacccgg catctgtgcc ggctctaccg
      961 gcagagcccg gacccctggg gtcatgcgac gcgcgcctat gagcgggcaa agttcgcgcg
     1021 gaccatcgcc gccctcgacg gtcgccgctt tcgccaggcg gtcgagatcg gttgcggcat
     1081 cggtgtcctg acggcgcttc tggcacggcg ttgcgaggcg ctcctcggca tcgactgcgt
     1141 cgcaagcgcg gtcgcaagcg cacgtctccg cctcgccggt cagtcgcatg ttcggcttgg
     1201 cgtcggcgtg gcgccggacg acctgcccga cctgtcaccc gatctgatcg tgctctcgga
     1261 ggtgctctac ttcatgaccc cggccgagat catccggctc gccgcctggt gcgatgccca
     1321 tgcggcgccc gcggcccgga tcgtcatcgt ctcgtggaag gggccgacag gcgaggccct
     1381 ctcgggcccc gcctccgccg cgctcctgtg caggccgctg gtcggctggt ctcaccgctc
     1441 cctggacggc gacggctatc ggatcgaccg gctcgatcgg ctggatggct gaaaggctca
     1501 gcggctcatc gtccgctcgg ccttgcgctg ggcgctggcc agctcgccct ggatgtcgcc
     1561 cccctggatg atcgactgca gggccacgac caggtcgtcc atcaccgcat agaacttcgg
     1621 gctcttgatc gggttgtcgt tggtgtcggg cagggcctgg acgaagacct gggtataggc
     1681 gtcctcgccc atcacgccgc tggtcaaaag cggcatgctg ccggtcgaga gctggctcga
     1741 ggcctcgaaa taggccgtcg tgatctcggg gtcgaagatc acgtggcgga tgaactcggc
     1801 cgccaggtcc ttgtgctcgg agccggcgga gatggtgatc tcgtgcgggt tgccgatcgt
     1861 gcgccgcttg cccgacgggt ccatgggcat cggtgccgcc cagatgtcgc cgtcggcggc
     1921 ggtggtgagc ttgccgcccg agaggttgtt gaagaggccg cgcccccagg ggccctcgaa
     1981 gatgaagccg gcgcggccgg cggccatggt gttgcgcgtc tcgtcgatgg tgaagccgga
     2041 aggcgcgcag cccgaatcgg tgagctccct gacccattca taggcggcgg ccgcctcggg
     2101 tgtgtcgaag ctgacattgc catcggcatc gacgatgtcg ccaccctggc cgtagatgat
     2161 cgggatggtc cactgggccg agttgggatt gcgcgcggtg cgcagcgcga cgccgtagat
     2221 cggggcgcca tccacgtcgg gcagcgcgca gatcgccttc gcggcctcga gcagctccgg
     2281 ccaggtctgc ggcggggcgt cggggtcgag cccggcctgc tccatgaggt tgcggttgta
     2341 gaacatgatg atcggcccgg ggacccaggg catggccatg atctggccgt cgaaggtcac
     2401 cgagtcgagc gaggactgcg ggatctgcgc ccactcctcg gcggtgaagt agtcgttcag
     2461 gggctcgagc gcgccgatgt cgttgagggc cgggacccag cccgcgatca ggtggatgtt
     2521 gtccggggcg ttgccggcga gggaggcgac ggtcgcctgc ttcagggcct ggtcgaacgg
     2581 cacgtcgttg aggatccagt cgacgtccgg gaacttctcc tcgaagccgg tctcgatggc
     2641 cccgacggcc gccgcgtggt gcgggtagag attgatccag ccgctgtagt tcagctcggt
     2701 cttgtcctgg gccgccgcac cggtggcggc gagcaccaca gccgcggcga gcgtggccat
     2761 cttcttgtcg agcatccaaa aagcctcctt ctttctcgtg gttcagccct tcacggcgcc
     2821 ggcggtgacg ccgctcatga aggttcgttg gaagacggtg aagagcagga agatcggcag
     2881 cgaaaccatg accgaaagcg ccatcatctc ggaccaggcc ccctcgaact cggtcaggaa
     2941 cgacatcgag ataccggccg ggagcggccg catgctggtc gaggagacga aggtcaggcc
     3001 gaacagcagc tcgttccagg acgtgacgaa gacgaagatc gccgacgccc cgagggcggg
     3061 gcgcgccatc ggcaggacga tgcgaaagag cgtgtaggcg cggctcgcac cgtcgacaaa
     3121 ggccgcctcc tcgacctcga cgggtagctg gtcgaagaag ttcttcagga gccagacaca
     3181 gaccggcagg gtgatcacgg tgtggccgat gaccagcccg atataagtgt tcagcaggcc
     3241 gagctggacg aacatcaggt acatcgagat catcaagagc gggatcggaa aggccaggga
     3301 agccatgaag aacatcagga tggcgttgcg gccgggcagg cggtagcgcg agaggccgta
     3361 ggcggccagc gcaccggcgc cagtggcgag gatcgcggag agcaggccga cgatcacgct
     3421 gttgaggaag tacctgccga gcaggcccgt gaactgggtg gtgtacgact cgaccgtcgg
     3481 attgcgtgga atgaactgcg gcgggtagct gaagacgtcg gcgagcggct tgaacgaggt
     3541 cgacagcatc cagacgatcg gcaggagcag gatcgcaagg gacgccacga cgagggcgtg
     3601 caggctgatg ctcgtcgcca gcctcgacag ccggtagacc acctcgctgc gccggcgcgg
     3661 cagcggcttc gatcccgtgc gcagggcctc ggcgttcatg ccaggttccg gcgcagggcc
     3721 acgcgcacgt agaggagcgc gaaggccatc agcaggatca gccagacagt gccgatggcc
     3781 gaggcgaggc cgaaatccca gtcctcgaag gccgtcttgt aggtgtagag cgagagcacc
     3841 atcgtggcgt tgaccggccc accctgggtg aggacgaaga tcatcgtcat ttcctggaag
     3901 aagcgcaccg cctgcaggag cgccgccacc aggatgatgt tctgcatcaa gggcaggatg
     3961 acgtagcgct gccgctgcag gaagctcgcc ccgtcgatcg ccgcggactc gaccagctcg
     4021 gaggggatgc cctgcagggc ggcgaggcag atgaccatga tgaacgggat ggcgcgccag
     4081 gcgtagccgc tgatgatcgc ccattgcgag gccttctggt cgaacatcca tttgaccggc
     4141 gtctctatca gccccacctg catgagcaga tagttgacga ggccgtagtc ctggctgaac
     4201 agccagcccc agagaatggc gatgacgatc cagggcacca cccagacctg gaagatggcg
     4261 gcgcggaaga tggcagcggc gcgaatgccc gagatcagga gcgtggccag gccgtagccg
     4321 caggcggtgg cgatgatcgt ggcagcgccc acgatgaaga ccgtgttcca caggacttcc
     4381 cagaactcgc cgtcctcgag gaggtagacg aagttctcga gcccgacgaa ggaccggccg
     4441 gagctgagcg ggctggcgtc ggtgaaggcg aggccgacac tgttcaccac gggatagacg
     4501 atgaagaggc cgacgagcag aatggacggc gcgaagatcg ccgccaggaa gaccggcgcc
     4561 tgccttggtg agaagcccaa accgcctccc tgctgaacgg ctgtccgccc ggtatccggc
     4621 aagtgcgccg ttgataaccg tcggttattg ctggcttgtc ggttagtgct tgtcgcagcg
     4681 ggctggcctc gtcaagacaa atgtcgtggg tcggctgcgg tcgaatcgag agcagatggg
     4741 agaatcgaat ggcatcggcc cgaaagccca acgtcatcct gatcgtcgcc gatgaccttg
     4801 gctacggcga tatcggcgcc ttcaacgagg gccgggtggc gacacccaat ctcgaccggc
     4861 tggtcagtga gggtgtcacc tgccgccagc actatgccgc ctcgccgatc tgcgcaccgt
     4921 cgcgcgcggc cctgctgacc gggcgctaca accaccgcac cggtgcgatc acccagcatg
     4981 agatccacgg gctggaccgc atcgcgctcc gcgaggtgac gctggctgat cgcttccgcc
     5041 atgccggcta cgccaccggg ctcgtcggca agtggcacaa cggcacgttc gaccggcgct
     5101 acgagcccaa tgcccgcggc tttcaggagt tcgtcggctt ctgcggcggc tgggtggact
     5161 actacgacta cacgcttcgg gtcgacgacg gcatccgacg ctccgacggg cgctacatga
     5221 cggacgtgct taccgaggag gccctgggct tcgtcgagcg ccacaagcga gagcccttct
     5281 tcctccacct cgcctacagc gcgccgcatt cgcccttcca ggcgccggcc ggcatggccg
     5341 agccgcatct ggagaagggc ctgaaccgga tcgtcgccac cacctacgcc atgatcgagc
     5401 gcatggacga gggcatcggc cggctgctcg acaggctcga cgcggaaggg ctggccgagc
     5461 agaccatcgt cctcttcacc agcgacaacg gcccggcctt cttcaacccg ccctacatgc
     5521 tcgagcccgg cgaggacacg ttcaacgaac ggttcaacgc cggcctcagg ggcagcaagg
     5581 gctggatcta cgagggcggc atccgggtgc cgatgctcct tcgctattcc gagcgggttc
     5641 cggccggatc caccaatgac gacctcatcc agttcgtcga ctgggtcccg acgctgatgc
     5701 gcctggccgg gcttgcgccg atggacgcac cgccgctcga cggcagcgac gtctcggcct
     5761 gcctcatggg cgagccggcc gacctgcccg accagcagtt ctggcagtgg aacttctaca
     5821 tgccctgcat cggcaccaac gcggcgatgc gcgacggttc ctggaagctg atcaggccga
     5881 tgatgaaggg cacccgcttc ttcagtcccg agctctacgc gacgcccgat gacgcggccc
     5941 tcgccaaggc cttcgtcgcg gcggacatcg cgcacaagcg ggacccgtcg tcgattcgcg
     6001 agctggtgcc cgtgccgcgg attcgcctgc cggcgcccga gccgcttgag ctctacgacc
     6061 tggccagcga ccccggcgag cagcacgacc tcgccgccca gcatccggac cgggtggcgc
     6121 ggatgcaggc caggctcgag gactggttcg accgggtcga agccgaccgt cggacgaccg
     6181 ccgaggccgc cgccgtagcc taatatcgcg tcagccgcgc ggtggtggcg gggtcatcgc
     6241 cagcatggcg aggagaccgc cgtcgacctt gaggtcggcg ccggtgatcc aggcggagcg
     6301 cggcccggcg aggaaggcga tcgcctcggc gatatcgtcc ggctggccca gccggccgag
     6361 cggcaggcgg cccgcctgct cggtcagcac cgcgtcgccg ccgcggtcgg gaaacatcgt
     6421 ctcccaggac ttccgcagca agggcgtgtc gatcgggccg gggcagaccg cgttgacgcg
     6481 gatcccgtcc ggcccgtgat tgagcgccat gccacgggtc atgccgagca gcgccgtctt
     6541 cgtcgtcacg taggcgagcc gttcgccgag cgtggcgaag gagtggaccg aggcgacgac
     6601 aacgatggcg ccaccgccgc gcttcttcat ctccggaatg ccgtgcttgg cgccgagcat
     6661 gacggcgtcg agattgatcg ccatgacccg ccgccagtcc tcctcggcga cctcgaccgc
     6721 cccgccgaac gtgccgacgc cggcattggc gaccaggatg tcgagcccgc cgaagcgatc
     6781 gaccgccagc ttcgtcagcc gctcggcgct gacggactcg gccagatcga gcgccatgcc
     6841 ggccatggag aggcctgccg cctcggcctc ggccagggtg gccgccacgt ccttctcgtt
     6901 cgacgagcag atcgcgaccg aagcgcccga cttcgcgagg cgccaggccg tggccttgcc
     6961 gatccccctg gtggcaccgg tgacgatcgc caccttgccg gcaaactcgc tgtcactcat
     7021 ctggccaggt ccccgtcaac tgccggcacg acgcaacgaa aacccagatg gcggatggcg
     7081 gtgtcgatcg cctggccctg ccgcgccgcc ggtcgatagc gcaggcaata ctggtcggtg
     7141 cagaggaagg agccgccctt gaccaccttg cgcgggattt gcagatgcgg cgcgaagggg
     7201 tcgatgctgc cttccctcgt gccgccatga ggatcgctcg gcacgcagca gggcttggcg
     7261 gcgtcaggcg cgtgccggtc a
//
LOCUS       DAOTFI010000234         7263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12268_length_7263_cov_6.997225, whole genome shotgun sequence.
ACCESSION   DAOTFI010000234 DAOTFI010000000
VERSION     DAOTFI010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12268_length_7263_cov_6.997225"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="P5558_19420"
     CDS             complement(<1..584)
                     /locus_tag="P5558_19420"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRY26546.1"
                     /translation="MRPLIVHHPTYVAPLPAGHRFPMPKFGALFALLRAKGLAPASRL
                     REAEPVGPADLALAHEPAYVAAVLGQRLGPAELRRLGLPLTAAVVARSRAAVGGTLLT
                     ARLALAHGLACNTAGGSHHAFAGFGAGFCVFNDVAVAARVLLAEGAVRRVMVIDLDVH
                     QGDGTAAILGSDPALFTLSVHCRTNFPARKERST"
     gene            719..1837
                     /gene="rfbG"
                     /locus_tag="P5558_19425"
     CDS             719..1837
                     /gene="rfbG"
                     /locus_tag="P5558_19425"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HRY26547.1"
                     /translation="MTKIDPTFWLNRRVLVTGHTGFKGSYLAAWLSELGADIVGVAHP
                     PAPPAGLFALAGLDQRLLNVTADVRDRARLEHLVVASSPEIVFHLAAQPVVLDGLKRP
                     LDTFDTNIQGTLNILQAARRAPDLRAMVVVTSDKAYRPSTEPRGEADHLGGIEPYSAS
                     KACADIIAEAYRHCYLSPEDGIGLATARAGNVIGGGDFAADRLVPDLVRGIAAGRTVP
                     IRHPDAIRPWQHVLDAVHGYLLLAEALATAPRRFARAWNFGPVQAPWPVRDVADRLVA
                     RLGGQWAAVPMAGRHETPVLRLDPTAAIEELGWRPRLDTAAAVDWTAEGYRRLEAEAS
                     GRFVLDQIERFSDLRPVGEVAGRGRPATGQPAEAHAHA"
     gene            1830..2642
                     /locus_tag="P5558_19430"
     CDS             1830..2642
                     /locus_tag="P5558_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRY26548.1"
                     /translation="MPESTPVFILCGGQGTRLGEAGRLRPKPLIEVGDQPLVIHIMRT
                     YRRFGFRRFILCTGHRHDVISSYFLDLAGIANDFSIDLATREIAYHQSSGAPDWHVTI
                     AQTGKATMTGARIARAAARYLGDAEHFAITYGDGLTDADLGAEFAHHLDHDRLGTVLA
                     VNPPCQFGRFDLAAGELQGFVEKPLDEQHWINGGYFFFRRGFLDYLDTDDGCVLEREP
                     LQRLAADGELELYRHKGFWSCVDTVADRDRVAALYEAGASPWLGPSERELAR"
     gene            2642..3217
                     /gene="rfbC"
                     /locus_tag="P5558_19435"
     CDS             2642..3217
                     /gene="rfbC"
                     /locus_tag="P5558_19435"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRY26549.1"
                     /translation="MRLVERPLAAAALLATEPHTDERGYFARAFCREALARLGFECAI
                     AQVNHSFSAKARTLRGLHFQVGRAAETKLVQCIAGAIFDVIVDVRPNSRTLGRWRGME
                     LSAANRRLLVVPPGFAHGFLTLTDHTLVTYLVTEAYEPSLDRGVAFDDPVIGIDWPVP
                     PLVVSARDRGHPPFDPERLWRAEPEMAGEPV"
     gene            3217..4176
                     /locus_tag="P5558_19440"
     CDS             3217..4176
                     /locus_tag="P5558_19440"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26550.1"
                     /translation="MRVLLTGASSFSGLWLARALVTAGAVVTAVARGHPERYEPLRRL
                     RIERVARLATTRFEAAFGSAAFLELIGREEPFHVLCLHHAEVGDFRRPDYDALAAARA
                     NTAGIEPVLERMRRRGLTRVVLTGSVFEADEGDGDRPLEAIGAYGLAKTLTTQVVRHA
                     AAAAGVELLKVTLPSPFGAFQAGGLADHLLRAWQASEVPSLRHPERVRDFVPIDLLAA
                     HYAALALGRIQPLAGGRANPSGHVLSCAAFAERLAQEMRPRLGAACRFALADQPVADG
                     EPQSRFNTEPLPALFDRAAQARSWDRLALHYRARARGWMEQAA"
     gene            4282..5220
                     /locus_tag="P5558_19445"
     CDS             4282..5220
                     /locus_tag="P5558_19445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY26551.1"
                     /translation="MVQPSIGVVVVTHRARRLLDGCLQPLLASPLRPQILVVNSSSHD
                     GTVEKAWDLGAETWTIPRESFDHGRTRELARQRLGTAITVMLTPDAHATGSDFLERLT
                     APLRDGRAAVAYGRQLPRPGADRIEAFNRSFNYPAQSQLRSLADWPTFGAYTHFCSNS
                     CAAWSSSALDEIGGFPPTLVSEETIAAARLLERGHRIAYVAEATVHHSHSSGLVADFR
                     RQFDVGHARRPYRHLLLGRERDEARGLAYTKGLLATLAREAPGLLPYALLHTAGRYAG
                     YRLGLGGARLPRRLKALMSGQDFYWRRPAGTLAGAG"
     gene            5226..6365
                     /locus_tag="P5558_19450"
     CDS             5226..6365
                     /locus_tag="P5558_19450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY26552.1"
                     /translation="MRVGLLTNNVMPPREGIGRHVLEIAKRLPRHGVEPLIVARGQGL
                     RWQSLEVEGVPVRLFPYLPVRPLHQEALRLVLQRWLDQGAEGCDALHLHLPLLPPLRT
                     ALRRVVTFHSPLLSDTAAIGEPGLKAALIKANARLFSQAYEHAHIRDAAALVAVSANV
                     RDELARHYRLLGRRVAVIGNGVDTGVFTYRGGPRPPDADGRRSVLYVGRLGYRKGLHR
                     LLDAFACLGDGRLRLELAGEGPLLEKLQAQARNLGIAERVCFLGFLDGPALLAALHRA
                     AVLVNPADYESGPLTLLEAMAGGTPVVTTSTGLAAELPEPRPCVVAERNPLDLATAIA
                     RVLADPEAAAAMARQARRLVLERFAWDDVVRRLVDRYGHGERLAA"
     gene            6362..>7263
                     /locus_tag="P5558_19455"
     CDS             6362..>7263
                     /locus_tag="P5558_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26553.1"
                     /translation="MSELLPVVVEQPRDLIVCFGFDLAKRRRQPWHVAHGLAQGLAAR
                     GRAVTIATDVAAEDDRSADYIVHGCGRLMQRGRPTPALRRFAEALAPARIYILTGTAA
                     LARMARLDLGAPTELIVASPLLRLREILAVGPLELWRERRLMLLPLVNALLPGCLVRA
                     GYRRSGAVRPIYLSEAGRERFRAIGLPPGPVLRPQVEAPPIMLRPRLLAPEAVPVVAY
                     LGPPLALRGAHLALGAFEAAVARGLDARLLLLLRPDVPPAVMRRFVERVARSPAGGRI
                     EVTTEMLGVAELARAIAGVDVFLLP"
BASE COUNT          933 a         2682 c         2587 g         1061 t
ORIGIN      
        1 tgggtgctgc gctccttgcg cgccgggaag ttggtccggc aatgcaccga gagggtgaag
       61 agggcggggt cggatcccag gatcgccgcc gtgccgtcgc cctggtggac atcgagatcg
      121 atcaccatca cccggcgcac ggcaccctcg gcgagcagca cgcgggcggc cacggcgaca
      181 tcgttgaaga cgcagaagcc ggcgccgaag ccggcgaagg cgtgatggct gccgccggcg
      241 gtgttgcagg cgaggccgtg ggcgagcgcg aggcgggcgg tcagcagcgt gccgccgacc
      301 gcggcccggc tgcgggcgac gacggcggcg gtcagcggca ggccgagccg gcggagctcc
      361 gcgggcccga gccgctggcc gagcacggct gccacatagg ccggctcgtg ggccaaggcg
      421 aggtcggccg ggcccacggg ctcggcctcg cgaagccgac tcgcgggggc caggcccttc
      481 gcccggagca gagcgaaaag cgcgccgaac ttgggcatgg ggaagcggtg cccggcgggc
      541 agcggcgcca cataggtcgg gtggtggacg atcagcggcc gcatcgcggc cctccagggg
      601 tcaattcgcc gggcatttcg cgcaaatgct tgcttctcgg ctaattaaat cataggttga
      661 attcaaccga tcagacttgc gctctctgcc aagaaagaaa cttcggaaaa ccaatgacat
      721 gacaaagatc gatccgactt tctggttgaa ccgtcgtgtt ctcgtgactg gccacacggg
      781 cttcaaggga agctatctcg cggcctggtt gagcgagctc ggcgccgaca tcgtgggcgt
      841 ggcccacccg ccggccccgc ccgccggcct gttcgcgctc gccgggctcg accagcgcct
      901 gctcaacgtt accgccgacg tgcgcgaccg ggcacggctc gagcatctgg tggtggcgag
      961 ctcgcccgag atcgtcttcc acctcgcggc ccagcccgtc gtcctggacg ggctgaagcg
     1021 gccgctcgac accttcgaca ccaacatcca gggcaccctc aacatcctcc aggcggcccg
     1081 ccgcgccccc gacctgcgcg ccatggtggt ggtcaccagc gacaaggcct acaggccctc
     1141 gaccgagccg cgcggcgagg ccgaccacct cggcgggatc gagccctaca gcgccagcaa
     1201 ggcctgcgcc gacatcatcg ccgaagccta ccggcattgc tacctttcgc ccgaggacgg
     1261 catcggcctc gccaccgcgc gggccggcaa cgtcatcggc ggcggcgatt tcgccgccga
     1321 ccggctggtg ccggacctgg tgcgcggcat cgccgccggg cggacggtgc cgatccgcca
     1381 tccggacgcc atccgcccct ggcagcacgt gctcgatgcg gtgcacggct acctgctgct
     1441 ggccgaggcg ctggcgacgg caccccggcg cttcgcccgc gcctggaact tcggcccggt
     1501 gcaggcgccc tggccggtgc gcgacgtcgc cgaccggctg gtggcccggc tgggcggtca
     1561 atgggcggcg gtgccgatgg ccggccggca cgagacgccg gtgctcaggc tcgatccgac
     1621 cgccgccatc gaggagctcg gctggcggcc caggctggac acggccgccg ccgtcgactg
     1681 gacggccgag ggctaccgca ggctcgaggc cgaggcgtcc ggccggttcg tcctcgacca
     1741 gatcgagcgc ttctccgacc tccggccggt cggggaagtc gcgggccggg ggcgcccggc
     1801 gaccggccaa cccgccgaag cccacgccca tgcctgagag caccccggtc ttcatcctct
     1861 gcggcggcca gggcacccgg ctcggcgagg cgggccggct ccggccgaag cccctgatcg
     1921 aggtcggcga ccagccgctc gtgatccaca tcatgcggac ctaccggcgc ttcggctttc
     1981 gccggttcat cctctgcacc ggccaccgcc acgacgtgat cagcagctac ttcctcgacc
     2041 tcgccggcat cgccaacgat ttcagcatcg acctcgccac ccgcgagatc gcctatcacc
     2101 agtcgtcggg cgcgccggac tggcacgtca ccatcgccca gaccggcaag gcgacgatga
     2161 ccggggcccg gatcgcccgg gccgcggccc gctacctggg cgacgccgag catttcgcca
     2221 tcacctatgg cgacggcctg accgatgccg atctcggcgc cgagttcgcc caccacctcg
     2281 accacgaccg gctcggcacg gtgctcgcgg tcaacccgcc ctgccagttc ggccgcttcg
     2341 acctcgccgc cggggagctc cagggcttcg tcgagaagcc gctcgacgag cagcactgga
     2401 tcaatggcgg ctacttcttc ttccgccggg gctttctcga ctacctggac accgacgacg
     2461 gctgcgtgct cgagcgcgag cctttgcagc gtctcgccgc ggacggcgag ctcgagctct
     2521 atcggcacaa ggggttctgg tcctgcgtcg acacggtggc cgaccgcgac cgggtggccg
     2581 ccctctacga ggcgggtgcc agcccctggc tgggtccgag cgagcgggag ctcgcccgct
     2641 gatgcgcctg gtcgagcgcc ccctggcagc ggcggccctc ctcgccaccg agccgcacac
     2701 ggacgagcgc ggctacttcg cccgcgcctt ctgccgcgag gcgctggccc gcctcggctt
     2761 cgagtgcgcc atcgcgcagg tcaaccactc gttcagcgcg aaggcccgga ccctgcgcgg
     2821 gctgcatttc caggtcgggc gggccgccga gaccaagctc gtccagtgca ttgcgggggc
     2881 gatcttcgat gtcatcgtcg atgtgcgacc caactcgcgg actctcggac gctggcgcgg
     2941 gatggagctt tcggcagcca accggcgcct tctggtggtg ccgccgggct tcgcccacgg
     3001 gttcctgacg ctgaccgacc ataccctggt gacctacctg gtgaccgagg cctacgagcc
     3061 ttcgctcgac cgtggggtcg ccttcgacga cccggtgatc ggcatcgact ggccggtgcc
     3121 gccgctggtc gtctcggcgc gcgatcgcgg ccatccgccc ttcgacccgg agcggctatg
     3181 gcgggccgag cccgagatgg ccggcgagcc ggtctgatgc gggttctcct caccggggcg
     3241 agcagcttca gcgggctctg gctggcgcgg gcgctggtga cggcgggggc cgtggtgacg
     3301 gcggtggcgc gcggccaccc cgagcgctac gagccgctcc gacggctgcg gatcgagcgg
     3361 gtggcgcggc tggccacgac gcggttcgag gcggccttcg gcagcgccgc gttcctcgag
     3421 ctgatcggga gggaagagcc cttccatgtg ctttgcctgc accatgccga ggtcggcgat
     3481 ttccgccggc ccgactacga tgcgctggcg gcggccaggg ccaacactgc cgggatcgag
     3541 ccggtgctcg agcggatgcg gcgccgcggg ctcacccggg tggtgctgac cggcagcgtc
     3601 ttcgaggcgg acgagggcga cggcgaccgg cccctggagg cgatcggcgc ctatgggctc
     3661 gccaagacgc tgacgacgca ggtcgtgcgc cacgcggcgg cggcggcggg cgtcgagctc
     3721 ctgaaggtca ccctgccgag ccccttcggc gccttccagg cgggcggcct cgccgatcat
     3781 ctcctgcgcg cctggcaggc aagcgaggtg ccgagcctgc gccatccgga gcgggtcaga
     3841 gacttcgtgc cgatcgacct cctcgcggcg cactacgcag cgctcgccct cggccggatc
     3901 cagcccttgg cgggcgggcg ggccaacccg agcggccatg tcctgagctg cgccgccttc
     3961 gccgagcgtc tggcacagga gatgcggccc cggctcggcg cggcctgccg cttcgccctg
     4021 gccgaccagc ccgtggcgga cggcgagccg cagagccgct tcaacacgga gcccctgccc
     4081 gcgctcttcg atcgcgcggc gcaggcacgc agctgggatc ggctggccct ccattatcgc
     4141 gcccgggcgc gcggctggat ggagcaggcg gcctgatggt tcttctattt atggttgatt
     4201 tcacgttgaa taattcgcat catcggcgcg tcccttccgt cgtccggccg tggcccaaat
     4261 cccttgccct ggagcaggca catggttcag cccagcatcg gcgtcgtcgt cgtcacccat
     4321 cgggcacgcc ggctgctcga cggctgcctc caaccgctgc tggcctcgcc gctccgcccg
     4381 cagatcctcg tcgtcaattc cagcagccat gacggaacgg tcgagaaggc ctgggatctc
     4441 ggggccgaga cctggacgat cccgcgggag agcttcgatc acgggcggac gcgcgagctg
     4501 gcgcggcagc ggctgggaac ggcgatcacg gtgatgctga cgccggacgc gcatgcgacg
     4561 ggcagcgatt tcctcgagcg gctcacggcc cccctgcgtg acggccgcgc cgccgtcgcc
     4621 tatggccgcc agctgccgcg gcccggggcc gaccggatcg aggcgttcaa ccgctcgttc
     4681 aactatccgg cccagagcca gctccgctcg ctcgccgact ggcccacctt cggcgcctac
     4741 acgcatttct gctcgaacag ctgtgctgcc tggtcgagca gcgccctcga cgagatcggc
     4801 gggttccccc cgacgctggt cagcgaggag acgatcgcgg cggccaggct gctcgagcgc
     4861 ggtcaccgca tcgcctatgt cgccgaggcg accgtccatc acagccactc gagcgggctc
     4921 gtcgcggatt tccgccggca gttcgacgtc ggccatgcca ggcgccccta ccgccatctg
     4981 ctcctcggcc gcgagcgcga cgaggcgcgc gggctcgcct acacgaaggg gttgctggcg
     5041 acgctcgctc gcgaggcgcc ggggctcctg ccctacgcgc tgctgcacac cgctggtcgc
     5101 tatgccggct accggctcgg cctcggcggc gcccggctgc cgcgccgcct caaggcgctc
     5161 atgagcggcc aggatttcta ctggcgccgg ccggcgggca ctttggccgg ggcgggttga
     5221 cgcccatgcg agtcgggctc ctgaccaaca atgtcatgcc gccgcgcgag ggcatcggcc
     5281 ggcatgtgct ggagatcgcg aaacgcctgc cgcggcacgg cgtcgagccc ctgatcgtcg
     5341 ccaggggcca gggcctgcgc tggcagtcgc tcgaggtcga gggtgtgccg gtccggctct
     5401 ttccctatct gccggtcagg cccctgcacc aggaggcgct gcggctcgtg ctgcagcgct
     5461 ggctcgacca gggtgcggag ggctgcgacg cgctgcacct gcacctgccg ctgctgccgc
     5521 ccctgcgcac cgccttgcgg cgggtggtga ccttccacag cccgctcctg agcgacacgg
     5581 cggcgatcgg cgagccgggc ctgaaggcgg cgctgatcaa ggccaatgcg cggctcttca
     5641 gccaggccta cgagcacgcc catatccggg acgcggcggc cctggtggcg gtgtcggcga
     5701 acgtcaggga cgagctggcc cggcattacc gcctcctcgg ccggcgggtc gcggtcatcg
     5761 gcaacggcgt cgacacgggc gtcttcacat accggggcgg gccgcgcccg ccggatgccg
     5821 acgggcggcg gagcgtgctc tatgtcggcc gcctcggcta tcgcaagggg ctgcaccgcc
     5881 tgctcgacgc cttcgcctgc ctcggcgacg gcaggctgcg gctcgagctc gccggcgagg
     5941 ggccgttgct ggagaagctc caggcgcagg cgcggaatct cggcatcgcc gagcgggtct
     6001 gcttcctcgg ctttctcgac gggcctgcct tgctcgcagc gctccaccgg gcagcggtcc
     6061 tcgtcaaccc ggccgattac gagagcgggc ctttgaccct gctcgaggcg atggccggcg
     6121 gcacacccgt ggtgacgacg tcaaccgggc tcgcggccga gttgcccgag cctcgaccct
     6181 gcgtcgtggc cgagcgcaac ccgctcgatc tcgccaccgc gatcgcgcgt gtactcgccg
     6241 acccggaggc ggctgccgcc atggccaggc aagcgcggcg gctcgtcctg gagcgctttg
     6301 cctgggacga cgtggtgcgc cggctggtcg atcgctacgg tcatggcgag cggctggcag
     6361 catgagcgag ctgctgccgg tggtcgtcga gcagccgcgc gatctgatcg tctgcttcgg
     6421 cttcgacctg gcgaagcggc gccggcagcc ctggcacgtg gcgcatggcc tggcccaggg
     6481 gctggccgcc cggggccggg ccgtcaccat cgccacggac gtcgcggccg aggatgaccg
     6541 gtcggccgac tacatcgtgc acggctgcgg ccggctgatg cagcgcgggc ggccgacgcc
     6601 ggcactccgc cgcttcgccg aggcgctggc accggcccgc atctacatcc tcaccggcac
     6661 ggccgccctc gcccgcatgg cacggctcga cctgggtgcg ccgaccgagc tgatcgtcgc
     6721 gagcccgctc ctgcggctgc gcgagatcct ggccgtgggg ccgctcgagc tctggcgcga
     6781 gcggcggctg atgctcctgc cgctcgtcaa tgccctgctg ccgggttgcc tggtcagggc
     6841 cggctatcgc cgctccgggg cggtgcggcc gatctatctg agcgaggccg gccgcgagcg
     6901 ctttcgcgcc atcggcctgc cgcccggccc cgtgctgcgg ccccaggtcg aggcaccgcc
     6961 gatcatgctg cgaccacggc tgctggcacc ggaggccgtg ccggtcgtgg cctatctcgg
     7021 gccccccttg gcgctgcgcg gcgcgcatct ggcgctcggc gccttcgagg ccgccgtggc
     7081 ccgcggcctc gacgcccggc tcctgctcct gctgcggccg gacgtgccgc cggcggtgat
     7141 gcggcgcttc gtcgagcggg tggcccggag cccggccggg gggcggatcg aggtcacgac
     7201 cgagatgctc ggggtcgccg agctcgcccg cgccattgcc ggggtcgacg tctttctcct
     7261 gcc
//
LOCUS       DAOTFI010000235         7172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12450_length_7172_cov_7.062948, whole genome shotgun sequence.
ACCESSION   DAOTFI010000235 DAOTFI010000000
VERSION     DAOTFI010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12450_length_7172_cov_7.062948"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..2044)
                     /locus_tag="P5558_19460"
     CDS             complement(50..2044)
                     /locus_tag="P5558_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HRY26554.1"
                     /translation="MTRKTHLETTRAHALEAAEDIGRNPMASPTMGDVINARFARRRV
                     LQGALAVSAISATLGPLALGASRPARAAAPSFGFAELAAGVDERHHVAPGYRAAIVVR
                     WGDPLFADAPEFDPMNQTAAAQLRQFGYNNDFLGYFPLDGSSEHGLLGVNHEYTNEEL
                     MFPGVAAQITKEDPLAYATRELVDIEMAAHGGSVVEVVRENGQWRYVRDSPYNRRVTM
                     AEGHTPMELTGPAAGHARMRTGADPEGRTVFGMLNNCAGGQTPWGTWLSAEENIHGYF
                     TGALPEGHAETVAFERYGVPGDWYAWGKFHDRFDISKEPNEPNRFGWVVEIDPYDPQS
                     TPKKRTALGRFKHECATPIVMADGRVALYMGDDERFEYIYKFVTSGRYDPDDRTANLT
                     LLDEGELSVARFNPDGTMEWLPLVHGQGPLTEANGFTSQADVVIGARIAADLLGATPM
                     DRPEDVEPNPKTGRIYAMLTNNTRRKAEQVDAANPRADNAFGHIVEMTPPDGDHSAGT
                     YTWEILVQCGDPSIADVGATFSSATTKDGWFGMPDNCAFDGEGRLWVSTDGNSDAGTG
                     RSDGLWAMETEGEARGTSKHFFRCPVGAELCGPVFTADDRSLFLAVQHPSDDTDAWAA
                     FGRLSTFEDPGTRWPDFQDGMPPRPSIVVVTKEDGGTIGS"
     gene            complement(2170..2976)
                     /locus_tag="P5558_19465"
     CDS             complement(2170..2976)
                     /locus_tag="P5558_19465"
                     /inference="COORDINATES: protein motif:HMM:NF018420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HRY26555.1"
                     /translation="MVVFRICPVLLLLLAWLPPAAPQGQAFDQPVDVELLLAVDVSGS
                     MDPDEHALQRQGYVAAIRSEAFRRTVRSGAYGRIALAYVEWAGIGRQAVVLPWVELAG
                     DADIDRVAGHLAQAPIAFIRGTSLSSILDFAGKHFDGNGFEGLRRVIDVSGDGPNNTG
                     GPVLDARDRVLKRGITINGLPIMIRPALHGSSTTPSLDEYFTACVIGGPGAFVLPVRE
                     IEAMAEAIRRKLVLEIAGAPVPAARIDQAQARPPVDCRIGERLRRFWMEP"
     gene            3160..5499
                     /locus_tag="P5558_19470"
     CDS             3160..5499
                     /locus_tag="P5558_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY26556.1"
                     /translation="MPAAPACVHAHTDKSTVRDPVCGMTVDLATTAHRTTHDGRSFGF
                     CSAGCLRKFETDPAHYLRPEEERRAAEAMPEGTIFTCPMHPEVRQVGPGDCPICGMAL
                     EPETVTADTGPSAEYLDMRRRFVLSAVLALPLVVIAMGRHLLPGLFGHAPVLLDWLEL
                     VLATPVVLWGGWPFFVRGWRSIVSGHLNMFTLIAIGTGIAWVYSVVAVLTPGLFPAAF
                     RDAHGSVGLYFEAAAVIVALVLLGQVLEIRAREATSGAIRALLDLAPDTARRIEADGD
                     ERDVPLAEIAVGDRLRVRPGDKVPLDGTVLEGGSAVDESMLTGESLPVEKGVGDPVTG
                     GTLNGRGSFVMEVERTGDDTTLRKIVEMVAAAQRSRAPVQRLVDRVAAWFVPIVIGVA
                     ILAFIVWALIGPAPALAHAMVVAVSVLIIACPCALGLATPMSIMVGVGRGAEAGVLIR
                     DAEVLERLAAVDTLVVDKTGTLTLGQPRLVGVETVEGGPDEATLLRLAAGIERGSEHP
                     LAAAIVDGARERGLEPAVAEDFDSITGKGVTGRVDGRQMALGNAALMAELGVEAGPLA
                     AVVARRQEAGETVMHVVLDGALAGLVAVADPIKEGAAKALADLRADGVAIIMLTGDNR
                     RTALAVAGRLGIAEVEAEVLPDRKSAVVERLRGEGRVVAMAGDGVNDAPALAAADVGI
                     AMGTGADVAVESAGVTLVKGNLAGIVRARRLSRATLRNIRQNLVFAFGYNSLGIPIAA
                     GVLYPVLGILLSPMVAAAAMSLSSVSVIGNALRLRRQGL"
     gene            5597..5836
                     /locus_tag="P5558_19475"
     CDS             5597..5836
                     /locus_tag="P5558_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRY26557.1"
                     /translation="MSEIGLCQAKMQLSELVERGETLTLTRHGRPVARIVPVAERRRG
                     LLKGRIGMAADFDETPGWLADAFEGGPGDGDLLKP"
     gene            complement(5985..6251)
                     /locus_tag="P5558_19480"
     CDS             complement(5985..6251)
                     /locus_tag="P5558_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRY26558.1"
                     /translation="MKKPWPVLKSDEEAERFVATADLTEYDFGQMVPMTFEYTAKDSR
                     VTMRLPGSLLDAVKARAKARGMPYQRYIRELLEREVRASGGGGR"
     gene            complement(6208..6507)
                     /locus_tag="P5558_19485"
     CDS             complement(6208..6507)
                     /locus_tag="P5558_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRY26559.1"
                     /translation="MQFDGFDWDDGNREKCRLHGVSIPEIEEALSVVRFVVDDPSPVE
                     KRYRTVGRTRAGRHVFAVFTMRGALLRPISVRYMHGKEVRTYEEALACAEERRGS"
     gene            complement(6603..>7172)
                     /locus_tag="P5558_19490"
     CDS             complement(6603..>7172)
                     /locus_tag="P5558_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY26560.1"
                     /translation="KIGPGTEAGVEMGPLVTREHLDKVRSYVDLGIEEGAKLVVDGRD
                     FRMQGYENGFFIGGCLFDGVTEDMRIAREEIFGPVLSVVRKPDYKSAVKIINDHEYGN
                     GTAIFTRDGDAARAFANEVEIGMVGVNVPIPVPMAFHSFGGWKRSLFGDHHTHGMEGV
                     RFYTKQKAITTRWPTGIRAGAEFKMPTMG"
BASE COUNT         1034 a         2522 c         2494 g         1122 t
ORIGIN      
        1 ccaagtccaa gccgaaatac agcatggccc tgcattcctt cacacgctct cagctgccga
       61 tcgtgccgcc gtcctccttg gtcaccacga cgatcgacgg ccggggcggc atgccgtcct
      121 ggaagtcggg ccagcgcgtg ccggggtcct cgaaggtgga gagccggccg aaggctgccc
      181 acgcgtcggt atcgtccgac gggtgctgca ccgccaagaa gagcgagcgg tcgtcggcgg
      241 tgaagaccgg gccgcagagc tcggcaccca ccgggcagcg gaagaagtgc ttggacgtgc
      301 cgcgcgcctc gccctcggtc tccatcgccc agagcccgtc cgaccggccg gtgcccgcgt
      361 cgctgttgcc atcggtcgag acccagagcc gaccctcgcc gtcgaaggcg cagttgtccg
      421 gcatgccgaa ccagccgtcc ttcgtcgtcg ccgagctgaa ggtggcgccc acgtcggcga
      481 tgctcggatc gccacactgc accaggatct cccaggtgta ggtgccggcc gaatggtcgc
      541 catcgggcgg cgtcatctcg acgatgtggc cgaaggcgtt gtcggcccgg ggattggcgg
      601 catcgacctg ctccgccttg cggcgcgtgt tgttggtgag catggcgtag atgcggccgg
      661 tcttcgggtt gggctcgaca tcctccggcc ggtccatggg cgtggcaccc aggagatcgg
      721 cggcgatgcg ggcaccgatc accacatcgg cctggctcgt gaagccgttg gcctcggtga
      781 gcggcccctg gccgtggacg agcggcagcc attccatggt gccgtccggg ttgaaccggg
      841 cgaccgacag ctcaccctcg tcgagcagcg tcaggttggc cgtgcggtcg tccgggtcgt
      901 agcgaccgct ggtgacgaac ttgtagatgt actcgaagcg ctcgtcgtcg cccatgtaga
      961 gggccacccg gccgtcggcc atgacgatcg gcgtggcgca ttcgtgcttg aagcggccga
     1021 gcgccgtccg cttcttgggc gtcgactgcg ggtcataggg atcgatctcg acgacccagc
     1081 cgaaccggtt cggctcgttg ggctccttcg agatgtcgaa gcggtcgtgg aacttgcccc
     1141 aggcgtacca gtcgcccggc acgccgtagc gctcgaacgc cacggtctcg gcatggccct
     1201 cgggcagggc cccggtgaag tagccgtgga tgttctcctc ggccgagagc caggtgcccc
     1261 agggcgtctg cccgccggcg cagttgttga gcatgccgaa gaccgtccgc ccctccggat
     1321 cggccccggt cctcatccgc gcgtggccgg cggccgggcc ggtcagctcc atcggcgtgt
     1381 ggccttcggc catcgtgacc cgccggttgt aggggctgtc cctgacatag cgccactggc
     1441 cgttctcgcg caccacctcg accaccgagc cgccatgggc cgccatctcg atgtccacca
     1501 gctcacgcgt ggcataggcc aagggatcct ccttggtgat ctgcgccgcc acgcccggga
     1561 acatcagctc ctcgttggtg tactcgtgat tgacgccgag caggccatgc tcgctgctgc
     1621 cgtcgagcgg gaagtagccg aggaaatcgt tgttgtagcc gaactgacgc agttgcgcgg
     1681 ctgccgtctg gttcatggga tcgaactcgg gcgcatcggc gaagagcgga tcgccccagc
     1741 ggacgacgat cgcggcccgg tagcccgggg ccacgtggtg ccgctcgtcg acgccggcgg
     1801 cgagctcggc gaagccgaag ctcggcgcgg ccgcccgggc cggccgcgac gcgccgaggg
     1861 cgagcgggcc gagcgtggcg ctgatggcgc tcaccgccag ggcaccctgc agcacgcgcc
     1921 gccgggcgaa gcgggcgttg atcacatcgc ccatggtcgg gctcgccatg gggttgcggc
     1981 cgatatcctc ggcggcctcg agcgcgtgcg ctcgcgtggt ttcgagatgc gttttcctcg
     2041 tcatttccat cccccgatcg tgatacgcgc caaggtcgaa gcgcatacgc tccgcctgaa
     2101 cgtcgtagtg tcatcaagca aatgtgacac atcgaagaca agtgaaatcg gcgacggctt
     2161 tcggcctcgt cagggctcca tccagaagcg ccgcagccgc tcgccgatgc ggcagtcgac
     2221 gggcggccgg gcctgggcct ggtcgatccg cgcggcgggg acgggcgccc cggcgatctc
     2281 cagcacgagc ttgcggcgga tggcttccgc catcgcctcg atctcgcgca ccggcagcac
     2341 gaaggcgccc gggccgccga tcacgcaggc ggtgaagtac tcgtcgagcg acggcgtggt
     2401 cgagctgcca tgcagcgccg gccggatcat gatcggcagg ccgttgatgg tgatgccgcg
     2461 cttcagcacc cggtcgcggg catcgaggac ggggccgccc gtgttgttcg gcccgtcgcc
     2521 ggagacgtcg atgacccgcc tcagcccttc gaagccgttg ccgtcgaaat gcttcccggc
     2581 gaaatcgagg atcgagctga gcgacgtgcc gcggatgaag gcgatgggcg cctgcgccag
     2641 gtggccggcg acgcggtcga tgtcggcgtc gccggcgagc tcgacccagg gcaggacgac
     2701 agcctgccgg ccgatcccgg cccattcgac ataggcgagc gcgatccgcc cgtaggcgcc
     2761 gctccgcacc gttcggcgga acgcctcgct ccggatcgcc gcgacatagc cctgccgctg
     2821 cagggcgtgc tcgtccgggt ccatcgagcc cgagacatcg acggcgagca ggagctccac
     2881 atcgaccggc tggtcgaaag cctggccctg cggcgccgcc ggcggcagcc aggcgagcag
     2941 caggagcagg accggacaga tgcggaagac gaccatgcgc gtccctcccc tggccccatc
     3001 ttcggaagtg gccggtcgcg gccggcggtc aagggccgac gcctgccgat catggcacgc
     3061 cacctgcccc ggcagcaccg ggattccccg cttgaccttc ccattgctgg aaggcttagc
     3121 ccgatcactg atggccgttg gggccaggga gaccgcgcca tgcctgccgc accagcctgc
     3181 gtccatgccc acaccgacaa gagcacggtg cgcgatccgg tctgcggcat gaccgtcgat
     3241 ctggcgacca cggcgcatcg aacgacgcac gacggccgga gcttcggctt ctgctcggcc
     3301 ggctgcctga ggaaattcga gacggatcct gcccactacc tccgcccgga ggaggagcgg
     3361 cgggccgccg aggcgatgcc cgagggcacg atcttcacct gccccatgca tcccgaggtg
     3421 cgccaggtgg ggcccggcga ctgcccgatc tgcggcatgg cgctggagcc cgagaccgtg
     3481 acggccgaca cggggccgag cgccgagtat ctcgacatgc gccggcgctt cgtcctgagc
     3541 gccgtgctcg ccttgccgct cgtggtgatc gccatgggcc ggcacctgct gccggggctc
     3601 ttcggccatg cgccggtgct gctcgactgg ctcgagctgg tgctggcgac gccggtcgtg
     3661 ctctggggcg gctggccctt cttcgtccgc ggctggcgct cgatcgtgag cggccatctc
     3721 aacatgttca cgctgatcgc catcggcacc ggcatcgcct gggtctacag cgtggtcgcg
     3781 gtcctgaccc ccgggctctt tccggcggcg ttccgcgacg cgcatggcag tgtgggcctc
     3841 tatttcgagg cggcggcggt gatcgtcgcc ctggtgctgc tcggccaggt gctggagatc
     3901 cgtgcccgcg aggcgacgag cggtgccatc cgggcactgc tcgatctggc acccgacacg
     3961 gcacggcgga tcgaggccga cggcgacgag cgggacgtgc cgctggccga gatcgccgtc
     4021 ggcgaccggc tgcgcgtccg cccgggcgac aaggtgccgc tcgacggcac cgtcctcgaa
     4081 ggcggcagcg ccgtcgacga gtccatgctg accggcgaat cgctgccggt cgagaagggg
     4141 gtcggcgacc cggtcaccgg cggcacgctc aacggccgcg gcagcttcgt catggaggtc
     4201 gagcggaccg gcgacgacac gacgctgcgg aagatcgtcg agatggtggc ggccgcccag
     4261 cgctcgcggg caccggtgca gcgcctggtc gatcgggttg ccgcctggtt cgtgccgatc
     4321 gtcatcggcg tcgccatcct cgccttcatc gtctgggcgc tcatcggccc ggcgccggcc
     4381 ctggcccatg ccatggtggt cgccgtctcc gtcctgatca tcgcctgccc ctgcgcgctc
     4441 gggctcgcga cacccatgtc gatcatggtg ggcgtcggcc gcggggccga agccggtgtg
     4501 ctgatcaggg acgccgaggt gctggagcgg ctcgccgccg tcgacacgct ggtggtcgac
     4561 aagaccggca cgctgaccct gggtcagccc cggctcgtcg gagtcgagac ggtcgagggc
     4621 gggcccgacg aggcgaccct gctccgcctc gccgccggga tcgagcgtgg cagcgagcac
     4681 ccgctggccg cggcgatcgt cgacggggcc agggagcgcg gcctcgagcc ggcagtggcc
     4741 gaggacttcg actcgatcac cggcaagggc gtcaccggcc gggtcgacgg ccggcagatg
     4801 gcgctcggca acgccgctct catggccgag ctcggcgtcg aggccggccc gctcgcggca
     4861 gtggtcgccc gccgccagga agcgggcgag accgtcatgc atgtcgtcct cgacggtgcc
     4921 ctcgcgggcc tcgtcgccgt cgccgatccg atcaaggagg gcgccgccaa ggccctcgcc
     4981 gatctgcggg cggacggtgt cgcgatcatc atgctgaccg gcgacaaccg gcgcacggcg
     5041 ctcgccgtgg ccggccggct cggcatcgcg gaggtcgagg cggaggtgct gccggaccgg
     5101 aagagcgctg tggtcgagcg gctgcgcggc gagggccggg tggtggccat ggccggcgac
     5161 ggcgtcaacg acgccccggc cctcgccgcc gccgacgtcg gcatcgccat gggcaccggc
     5221 gcggatgtcg cggtcgagag cgccggcgtc accctggtca agggcaacct cgccggcatc
     5281 gtccgcgccc gccggctgag ccgcgccacc ttgcgcaaca tccgccagaa cctcgtcttc
     5341 gccttcggct acaacagcct cggcatcccc atcgccgccg gcgtcctcta ccccgtgctc
     5401 ggcatcctcc tgagtccgat ggtggcggct gccgccatga gcctgagctc cgtctcggtc
     5461 atcggcaacg cgctgaggtt gaggcggcag ggcctttgat gcaccgagcc ccggccattc
     5521 aggccggggc tcgcttcaca tgccggccgc atgcaaccac attctcggag gttgcaacca
     5581 catcgtgagc gccgccatga gcgagatcgg cctgtgtcag gccaagatgc agctcagcga
     5641 gctggtcgag cggggcgaga cgctgacgct cacccgtcac ggcaggccgg tcgcccgcat
     5701 cgtgccggtg gcggagcgtc gtcggggcct gctgaagggg cggatcggca tggcggcaga
     5761 tttcgacgag acccccggct ggctggccga cgccttcgag ggcgggccgg gcgacggcga
     5821 cctgctgaag ccttgagcgc cgtcctcctc gatatccaca tcctgctccg gtggctggac
     5881 gacgacccgc gcttcggcgc ctatgaggtc gcgttgcagg cggggacgag tacgggaagc
     5941 gccgcaagcc cggcccgacc gggcattgcg tcgaccggac agagctagcg tccgccgccg
     6001 ccgctggcac gcacttcccg ctccagaagc tcgcgaatat agcgctggta cggcatgccg
     6061 cgggccttgg ctcgggcctt gaccgcatcc agcaggctgc ccggcagacg catggtcacc
     6121 cggctgtcct tggcggtgta ctcgaacgtc atcggcacca tctggccgaa gtcgtactcg
     6181 gtgaggtccg ctgtcgccac gaaccgctca gcttcctcgt cgctcttcag cacaggccag
     6241 ggcttcttca taggtgcgca cctccttgcc gtgcatgtac cgaacgctga tcggccggag
     6301 caacgcgcca cgcatcgtga agaccgcgaa aacgtggcgg ccggccctgg tgcgtccgac
     6361 ggtccggtac cgtttctcga ccgggctcgg gtcgtcgacg acaaagcgga cgacactcaa
     6421 agcttcctcg atctccggga tcgagacgcc atgaagccgg catttttccc gattgccgtc
     6481 atcccagtcg aacccgtcga attgcaccga ctgatctcaa tatatggcag ccaaacgtac
     6541 atacaataag cgatccagaa tggcctcgaa agcacacgcg ccggcgaggc cgcccccccc
     6601 cctcacccca tggtcggcat cttgaactcg gcgccggcgc ggatgccggt gggccagcgg
     6661 gtcgtgatcg ccttctgctt cgtgtagaag cgcacgccct ccatgccgtg ggtgtggtgg
     6721 tcgccgaaga gcgagcgctt ccagccgccg aagctgtgga acgccatggg caccgggatc
     6781 ggcacgttga cgcccaccat gccgatctcg acctcgttgg cgaaggcccg ggcggcgtcg
     6841 ccgtcgcggg tgaagatcgc cgtgccgttg ccgtactcgt ggtcgttgat gatcttcacc
     6901 gccgacttgt agtcgggctt cctcaccacc gaaaggacgg ggccgaagat ctcctccctg
     6961 gcgatccgca tgtcctcggt gacgccgtcg aagaggcagc cgccgatgaa gaagccgttc
     7021 tcgtagccct gcatcctgaa gtcgcgaccg tcgacgacga gcttcgcccc ctcctcgatg
     7081 ccgaggtcga catagcttcg gaccttgtcg aggtgctcgc gcgtgaccag cggccccatc
     7141 tccacgcccg cctcggtgcc cgggccgatc tt
//
LOCUS       DAOTFI010000236         7158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12480_length_7158_cov_5.807828, whole genome shotgun sequence.
ACCESSION   DAOTFI010000236 DAOTFI010000000
VERSION     DAOTFI010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12480_length_7158_cov_5.807828"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="P5558_19495"
     CDS             <1..411
                     /locus_tag="P5558_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26561.1"
                     /translation="DVALETIRTKGNGANWWQASPKALFLSRGSANVDAGEYYPHIFH
                     EGGAEGGGVWLSSPIVDGNFHPGGGTYAPAYVAGPPARWEWPNVNPPGGRGKLVTGRI
                     LAGSPPSPVVQSSDVGLAFRIVDKSALESLIAAL"
     gene            475..723
                     /locus_tag="P5558_19500"
     CDS             475..723
                     /locus_tag="P5558_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26562.1"
                     /translation="MANEQSRGPDSTSSMFFAITPHDTNPLERWTRAIHVGGAGVVKC
                     RNELGAWVEFTCPAGAVLPVRTQYVHTDSTATLMVGLA"
     gene            762..1484
                     /locus_tag="P5558_19505"
     CDS             762..1484
                     /locus_tag="P5558_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26563.1"
                     /translation="MAQPVGEGGGEPATAVTIAGTPATHGEGGAHTSHPVTGYNHAGG
                     TNDALAVIVSGRPQSGGAQAITVTWNGVAVPERVDTSDIAGLTANESFVWVGLLVGAA
                     SGVQALAVGFDKTQSDSVVQLVSLVNVDQAAPVVGVASSFDEAEATTVATAGRTGAST
                     ANLFLAGAGIAVGSSLSALTSGWAEVISGKSDAGTSTSTALLSTVARAPTASGADPLT
                     VTFADSTTRARALGLVEVKAAA"
     gene            1659..1922
                     /locus_tag="P5558_19510"
     CDS             1659..1922
                     /locus_tag="P5558_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26564.1"
                     /translation="MDRLARLLLNRLRPNELLSIVHEPGRVLIEFHPERGAAEVVELV
                     EGRRLVLARDDDAGTFERVEVSAVKLETAAANDPGRPDLAAAG"
     gene            1958..2293
                     /locus_tag="P5558_19515"
     CDS             1958..2293
                     /locus_tag="P5558_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26565.1"
                     /translation="MAADRSPGELRELLDLLGCRLHRDDLVIVTNHGRGRMTVEARIA
                     GKVALVEVPRRKSFIAWPAGRDFLDQETELSGPLAEEPRQEARPAAAGEGPKVVRLVP
                     RGPVAGEQA"
     gene            complement(2177..2611)
                     /locus_tag="P5558_19520"
     CDS             complement(2177..2611)
                     /locus_tag="P5558_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26566.1"
                     /translation="MLSLAEAASRVGKSKPTLQRAIKAGRLSASRDEHGAYLIDPAEL
                     ARVYPTSRAALAAVVAQPSQAEEFADLTHQIAMLEARLSMVTEERDYLRERIIGFERQ
                     TNQTLSLLAGDRPARDEPDDLWPLARRRRPGFLARLFGERAG"
     gene            complement(2988..3167)
                     /locus_tag="P5558_19525"
     CDS             complement(2988..3167)
                     /locus_tag="P5558_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26567.1"
                     /translation="MQIMLTSTGPGPLGQGMRGLDDFVTDRPSLFATVTLYTGWHDVT
                     ACVDQPAFERLVARA"
     gene            complement(3243..3575)
                     /locus_tag="P5558_19530"
     CDS             complement(3243..3575)
                     /locus_tag="P5558_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26568.1"
                     /translation="MKADARHAPTFSRAQARRIDEAGAIVDRLDIARKLIELHAPDNR
                     LALARQLLRQTADDVATMAGIEDETTAARRVSRGPFAGKRTWPSRRAAGSESLAPSSA
                     SLPERRVH"
     gene            complement(3930..4196)
                     /locus_tag="P5558_19535"
     CDS             complement(3930..4196)
                     /locus_tag="P5558_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26569.1"
                     /translation="MVKMPGQAEIDRFTDFLEQQRRFFDDMKNWQSLDRKALIRLLMA
                     DLEASCKAMQLVREFMTKLAELEIRAVRAETALAERADASVPPI"
     gene            complement(4190..4810)
                     /locus_tag="P5558_19540"
     CDS             complement(4190..4810)
                     /locus_tag="P5558_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26570.1"
                     /translation="MTIAIASGDRQRVVLVADTLAVQGDGHRADAFVSKVLPFAHCAA
                     AAVVRGHGGHKLAIHMAMNTRPALDGIGALPWPAILDRYVGDDQEQFDMLLAGWAPAA
                     GGLAIWRFGEATGYELAEVALPASVALPDFGVALDPVMADGAVIHAAEVLVDLANESA
                     RETLGAGEPDGAGGDLILTELTGGGIVQRKVKRLANHAALKEQMTW"
     gene            complement(4807..5880)
                     /locus_tag="P5558_19545"
     CDS             complement(4807..5880)
                     /locus_tag="P5558_19545"
                     /inference="COORDINATES: protein motif:HMM:NF018497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HRY26571.1"
                     /translation="MADRDISLRLAVTDAEASIRRLREFEAQGDQALGRIASASKPAS
                     AGLTAVDQSSIALQGTMARLVPALATFDTGLAGLAAGGILALGAGLAAGVRNAEAAER
                     SLLRLDGVLKATGGSAGLTAREITALGDQMERSTLAGGEAVVDAAAALATFKSVSGDT
                     FTDAIRLSQDLAAVFGGDLTSTASQLGKALEEPVRGLDCLREKGVTFSAAQREVIKSL
                     VETGDKAEAQRQILEADPAARRSGGGRRGARGRRQGDRGRECRSQGQILAGLVAGRQA
                     EADAIDEVKAARERLARLEDDRRRRIEDRGFDPSASVADFGAFKAGREADQAREAERM
                     AAVFIRPRPAAQARAVARLRALR"
     gene            complement(5998..6225)
                     /locus_tag="P5558_19550"
     CDS             complement(5998..6225)
                     /locus_tag="P5558_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26572.1"
                     /translation="MRVALASEVPVVSAAELQEAYPAESHADIRRYVALVRRKGISTE
                     NARWLVSFTGCVERAISRLEAAELPDRQRPN"
     gene            complement(6228..6785)
                     /locus_tag="P5558_19555"
     CDS             complement(6228..6785)
                     /locus_tag="P5558_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26573.1"
                     /translation="MAAKKLEAPTARLDEIGRIRARLAAIEPERDETERAPLSGAEIA
                     ERASAIADRLAMLAGADVLARYLIALQRRGSAHLVGEVVARLANPVEAAGQKAVPAAA
                     LLAAMDREGMVAWLTRIAAAEAADYPPALAEAERRQRVQILHEERLGLERDEVAAHWA
                     LEETGLAAPWRSGMSPALVLGLTEE"
     gene            complement(6788..>7158)
                     /locus_tag="P5558_19560"
     CDS             complement(6788..>7158)
                     /locus_tag="P5558_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26574.1"
                     /translation="QTRGSSACSSLNRCEPGLPNSKPASGVMQGHAQRPDTRPIEDPA
                     ETSKITLAKPGASTHEATGGRTRVLAEADHHAKEFHATREAIADLKRRRDIASAEARP
                     LNGLRTACAEHLRGRGWRET"
BASE COUNT         1038 a         2469 c         2531 g         1120 t
ORIGIN      
        1 gacgtcgccc tcgaaaccat ccggaccaag ggcaacgggg cgaactggtg gcaagcctcg
       61 ccgaaagcgc tgttcctttc ccggggctcg gccaatgtcg acgcggggga atactatccg
      121 cacatcttcc acgagggcgg ggcggaaggc ggcggcgtct ggctttcttc gccgatcgtc
      181 gacggcaact tccatccggg cggcggcacc tatgcgccgg cctatgtcgc cgggccgccg
      241 gcccgatggg aatggccgaa cgtcaacccg ccgggcggcc ggggcaagct ggtgactggc
      301 cggatactgg ccggctcgcc gccctcgccc gtggtgcaat ccagcgacgt cgggctggcc
      361 tttcggatcg tcgacaagtc ggcgctggaa tcgctcattg ccgcgctgta gaggataatg
      421 accggcgccg gatcaagtct gcacggcgcc ggctccttca acgggggcga gaacatggcg
      481 aacgaacaga gccgcgggcc ggactccacc tcctccatgt tcttcgcgat cacgccgcac
      541 gataccaacc cgctcgagcg ctggacccgg gccatccacg tcggcggggc cggggtggtg
      601 aagtgccgga acgagctcgg cgcctgggtc gagttcacct gtccggccgg cgccgtcctg
      661 ccggtgcgga cgcagtacgt tcacacggac tccacggcca cgctgatggt cgggctggcc
      721 tgatgctctc tctcgcgctg tcgctgccgg tcccggcggc gatggcgcaa ccggtcggcg
      781 agggcggcgg ggagccggca acggcggtga cgatcgcggg gacgccggcg acgcacggcg
      841 agggcggcgc gcacacctcg catcccgtca cgggctacaa ccacgccggc ggcaccaatg
      901 atgccctggc ggtgatcgtc tccggccggc cgcaaagcgg cggcgcgcaa gcgataaccg
      961 tcacctggaa cggcgtcgcg gtgccggagc gggtcgacac gtcggacatt gccgggctca
     1021 cggcgaacga gtcgttcgtc tgggtgggcc tcctcgtcgg ggcggcctcg ggcgtgcaag
     1081 ccctggccgt gggtttcgac aagacgcagt ccgattcggt cgtgcaactc gtctcgctgg
     1141 tgaacgtcga ccaggcggcg ccggtggtcg gcgtggcgtc cagcttcgat gaagcggaag
     1201 cgacgacggt ggcgacggcc ggccggaccg gggcgagcac ggcaaatctc ttcctcgccg
     1261 gggccggaat cgcggtcggc tcctcgctct cggccctgac aagcggatgg gccgaagtca
     1321 tctccggcaa gagcgacgcc gggaccagca cgagcacggc gctgctgtcg accgtcgcca
     1381 gggcgccgac cgcgagcggc gccgatccgc tcaccgtgac ctttgccgat agcacaaccc
     1441 gcgcccgcgc gctcggcctg gtcgaggtga aggcggcggc gtgatcgtcc agcggctcga
     1501 tgctgcggat cttcgcatgg cggccgaaga actccgccgg gccgtcgagc tactggccaa
     1561 tgtgatcgag cacctcgaaa cagccgagga tcattgccag gaacgatcta cccacagtag
     1621 aattgacatg gcagttattc taccatttgg ggatgcggat ggatcgactg gccaggctcc
     1681 tgctcaatcg gctgcggccg aatgaactgc tctcgatcgt ccacgaaccg ggccgcgtcc
     1741 tgatcgaatt tcacccggag cgtggcgcgg ccgaagtcgt cgagctcgtc gagggtcgcc
     1801 ggctcgtcct ggcgcgcgac gatgacgccg gcacattcga gcgggtcgag gtgtccgccg
     1861 tcaagctcga gacggcggcg gcgaacgatc cgggccggcc cgatctggcg gccgccggtt
     1921 gaaaggatcc tttcaaaagg cgggggctta ggaaaggatg gcggccgatc gttcgcctgg
     1981 cgagctgcgc gagctcctcg atctcctcgg ctgccggctg caccgcgacg acctggtgat
     2041 cgtcaccaat cacggccggg ggcggatgac ggtcgaggcc aggatcgccg gcaaggtcgc
     2101 cctcgtcgag gtgccgcgcc gcaaatcctt catcgcctgg ccggccggcc gcgacttcct
     2161 cgatcaggaa accgagctat ccggcccgct cgccgaagag ccgcgccagg aagcccggcc
     2221 ggcggcggcg ggcgaggggc caaaggtcgt ccggctcgtc ccgcgcgggc cggtcgccgg
     2281 cgagcaggct tagggtctgg ttcgtctggc gctcgaaacc gatgatgcgc tcgcgcagat
     2341 agtcccgctc ttccgtcacc atcgaaaggc gggcctcgag cattgcgatc tgatgggtga
     2401 ggtcggcgaa ctcctccgcc tggctcggct gggcgacgac ggcggccagg gcggcgcggc
     2461 tcgtcggata gacgcgggcg agctcggccg ggtcgatcag ataggcgccg tgctcgtcgc
     2521 ggctcgccga caatcggccg gccttgatcg cccgttgcag ggtcggcttg ctcttgccga
     2581 cgcgcgatgc agcctcggcc agtgacagca tttccgggcc tcctgccgtt gcacgccgtt
     2641 gcgcggcgct tcatcggcgg ttcatagacg attcgcgctt gccgggccac gggcaccggc
     2701 taggctggtg aatcgcgccg gacgggtgac cgacttctgg cgcatccatc gccgaaaaac
     2761 ctgggctgat tcgcgcgtcg aaagtcggtc ggcccgttgt ttttgctctt aagtccggta
     2821 acttttaatc cgcgggttct gggttcgagt cccagggggc gcaccaaaat caaagacttg
     2881 gcagcgtctc cctgagagag tcgccaaaag gtatcaccaa aggaccacag aaaacggaca
     2941 cagaatgggc cggcacccga aggcaccggc ccgatagaga gtgtgcgtca ggcgcgggcg
     3001 accaagcgct cgaacgccgg ctggtcgacg caggccgtca catcgtgcca gcccgtgtag
     3061 agcgtcacgg tcgcgaagag cgacggcctg tcggtcacga agtcgtcgag gccgcgcatc
     3121 ccttggccca gcggtcccgg cccggtgctc gtcagcatga tctgcacgtc ggaggcctcg
     3181 gcgatcttga accgcagccc cgtccagtgt gggggccgtc tcccatcggg ccatgacgct
     3241 cattagtgca ccctccgctc tggaaggctt gcgctggaag gtgcgagact ttcgcttccg
     3301 gctgcccgtc gcgacggcca ggttcgcttg ccagcgaatg gcccgcgtga gacccgccgc
     3361 gcggcggtgg tctcgtcctc gatgccagcc atcgtcgcca cgtcgtcggc cgtctggcgc
     3421 agcagttgcc gggccagggc gagccggttg tcgggagcgt gcagctcgat cagcttccgg
     3481 gcgatgtcca ggcggtcgac gatggcgccg gcctcatcga tgcggcgtgc ttgggcgcgt
     3541 gagaaagtcg gagcatgacg tgcgtcagcc ttcatggtgc taatcccacc gtgagggttg
     3601 gggcggcggc ggtgttggtg gcaccgcgcc gtcctccccc gccgcgcggg gcatcatgac
     3661 gctgcgggcg tcaacgagca atattctgcc gccgtgcctc tggcggccgc cccggcagcg
     3721 gcgtcgtcgt cgccaccgcg gcaaacgccg tcagggccgc gacaagcgcc gcctgggcct
     3781 cggcgtcgcc ctggtcgatc catcgctgaa cggccgtccc ggcggcccgc agggcggcct
     3841 ctggcgccgg ccgcctcatc gccgctggcc tgcccagatg gcgagggcga tcgcggccag
     3901 gagcaggatc gacggcacca ggccgggcat cagatcggcg gcacgctggc gtcggcacgc
     3961 tcggccaggg cggtctcggc gcggacggcg cggatttcaa gctcggccag cttggtcatg
     4021 aactcgcgga ccaactgcat cgccttgcag ctcgcttcga ggtccgccat caggagccga
     4081 atgagggcct tgcggtccag gctctgccag ttcttcatgt cgtcgaagaa ccggcgttgc
     4141 tgctccagaa aatccgtgaa gcggtcaatc tcggcttggc ccggcatctt caccatgtca
     4201 tctgctcctt cagtgccgcg tggttcgcca accgcttgac cttgcgctgc acgatgccac
     4261 cgccggtcag ctcggtcagg atcagatcgc cgccggcgcc gtccggctcg cccgccccca
     4321 gggtctcccg cgcggattcg ttggccagat cgaccagcac ctcggcggcg tggatcaccg
     4381 ccccgtcggc catcaccgga tcgagcgcga caccgaaatc gggcagcgcc acgctcgccg
     4441 gcaacgccac ctcggccagc tcgtagcccg tggcctcgcc gaaccgccag atggccagcc
     4501 cgcctgcggc aggcgcccag ccggcgagaa gcatgtcgaa ctgctcctgg tcgtcaccga
     4561 cgtagcgatc gaggatggct ggccatggca gcgcaccgat gccgtcgagg gcgggtcgcg
     4621 tgttcatcgc catgtggatt gcgagcttgt gcccgccatg gccgcgaacc acggccgccg
     4681 cggcgcaatg ggcgaacggc agaaccttcg agacgaaagc gtcggcccgg tgcccgtccc
     4741 cctgaacagc gagcgtgtcg gcgacgagga caacacgctg ccggtcgccc gatgctatcg
     4801 cgatggtcaa cgcagcgccc ggaggcgggc cacagcgcgc gcctgggctg ccggccgagg
     4861 ccggatgaag acggccgcca tgcgctcggc ttcgcgggct tggtcggcct cgcgtccggc
     4921 tttgaaggcg ccgaagtcgg ccacgctggc cgaaggatca aagccgcgat cctcgatccg
     4981 gcggcggcgg tcgtcctcca gccgggccag ccgctcgcgg gcagccttca cctcgtcgat
     5041 ggcgtcggcc tcggcctgtc gtccggccac cagcccggcg aggatctggc cctgcgaacg
     5101 gcattctcga cctcgatcgc cttggcgacg gcctcgtgcg cctcggcgcc ctcctccaga
     5161 gcgacgcgca gccgggtctg cttcgaggat ctgccgctgc gcctcggcct tgtcgccggt
     5221 ctcgaccagc gacttgatca cctcgcgctg cgccgccgag aaggtgacac ccttctcccg
     5281 caggcaatcg aggccgcgca ccggctcctc cagcgccttg ccgagctgcg aggcggtgct
     5341 ggtcaggtcg ccgccgaaga ccgccgccag gtcctgcgaa agccggatcg catcggtgaa
     5401 cgtgtcgccg gagacgctct tgaaggtcgc gagcgcggcg gcggcatcga ccacggcctc
     5461 gccgccggcc agggtcgagc gctccatctg gtcgccgagg gccgtgatct cgcgcgccgt
     5521 gaggccggcg ctgccgcccg tggccttcag cacgccgtcg agtcgcagca gcgagcgctc
     5581 ggccgcttcg gcgtttcgga cgccagccgc cagcccggcg ccgagggcca ggatgccgcc
     5641 ggcggcgagg ccggcgaggc cggtgtcgaa ggtcgcaaga gccggcacca ggcgggccat
     5701 ggtgccctgc aaggcgatcg acgactggtc gaccgcggtg agcccggccg aggcgggctt
     5761 ggacgcagag gcgattcgac cgagggcctg gtcgccctgt gcctcgaact cgcgaaggcg
     5821 ccggatcgat gcctcggcat cggtgacggc caggcgaagg gagatgtcac ggtcagccac
     5881 ggtgccctct caggaatatg cggcggacgg ctgggcattt cgcgcgccgg cggacagcgc
     5941 gcccggagcc gagcctgaag acgccgccgc gtcgcctgcc ctggccggtg tcttgcatca
     6001 gtttggtcgc tgccggtcgg gcagctcggc ggcctcgagg cggctgatgg cccgctcgac
     6061 gcagccggtg aaagagacca accaccgcgc gttttcggtg ctgatgccct tccgccggac
     6121 cagggccaca tagcggcgaa tgtcggcgtg gctctcggcc ggataggcct cttgcagctc
     6181 ggccgcagag acgaccggga cttcgcttgc cagtgccacc ctcattgtca ttcctccgtc
     6241 aagccgagca cgagcgccgg cgacatgccg ctgcgccagg gtgcggcgag gccggtctct
     6301 tcgagtgccc agtgcgccgc aacctcgtcg cgctccaggc ccagccgctc ctcgtgcaga
     6361 atttgaaccc gctgccgacg ctcggcttcg gccagcgccg gcgggtagtc ggcggcctcg
     6421 gcggcggcga tccgcgtcaa ccacgcgacc atgccttcgc gatccatcgc cgcgagcaag
     6481 gcggcggcgg ggacggcctt ctgtccggcg gcctcgaccg ggttggccaa tctggcgacg
     6541 acctcgccga cgaggtgagc cgagccccgc cgttgcagcg cgatcaagta ccgggccagc
     6601 acgtcggcgc ccgcgagcat ggcgagccgg tccgcgatcg cggatgcacg ttctgctatc
     6661 tcggcgccgc taagcggtgc gcgttcggtt tcgtcgcgct cgggctcgat ggccgcgagc
     6721 cgggcgcgga tgcggccgat ctcgtcgagg cgggcggtag gtgcttcgag cttcttcgcg
     6781 gccatggtta agtctcccgc cagccgcggc cgcgcaggtg ctcggcgcaa gccgtccgca
     6841 ggccgttcaa cggcctggcc tcggccgagg cgatgtcgcg gcggcgcttg aggtcggcga
     6901 tggcctcgcg ggtcgcgtgg aactccttgg cgtggtggtc ggcctccgcg agaacccggg
     6961 tccggccgcc ggtcgcctcg tgtgtggatg cccccggttt ggcaagcgtt atcttcgatg
     7021 tttcggcagg gtcttcgatc ggacgtgtgt caggcctctg agcgtggcct tgcatgacgc
     7081 cactggccgg tttgctgttc ggaaggccgg gttcacatcg gttcaaagag ctgcaagcgc
     7141 tcgagccccg ggtctgaa
//
LOCUS       DAOTFI010000237         7146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12513_length_7146_cov_6.683119, whole genome shotgun sequence.
ACCESSION   DAOTFI010000237 DAOTFI010000000
VERSION     DAOTFI010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12513_length_7146_cov_6.683119"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1731)
                     /locus_tag="P5558_19565"
     CDS             complement(52..1731)
                     /locus_tag="P5558_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY26575.1"
                     /translation="MTTTRRSGGEILIDELVRHGTDRIFGVPGESYLAALDALHGRND
                     IAFVICRQEGGAAMMAEADGKLTGRPGVCFVTRGPGATNASAGVHIARQDSTPMILLV
                     GQIAREARDREAFQEVDYRQMFGGIAKWVAEIEDARRIPEYLHRAFMTATTGRPGPVV
                     LALPEDMLTDEVMTEPGRPWQQVRAHPDPAAVETTMTWLGEAERPLVILGGGGWDQDA
                     VTALRRFVEANDLPTAVGFRRQDYLDNTHRCYVGDIGIGANPKLLERLDHADLLLVIG
                     ARLGEMTSDGYTRLGLPRPKQRMIQVLNGPEELGRVYQPDLLIQASPGPFVEALAARG
                     PLASRTWALGTADGHAAYLENIAPLEIPGNVQMPAIMAELRATLPAEAIVTNGAGNYS
                     AWCNRFYSYRAFRSQLAPTSGSMGYGLPAAIAAKLAHPDRPVVCFAGDGCFLMTGQEL
                     ATACQQRLAIVILVFNNGMYGTIRMHQEREYPTRVYGTELANPDFARLAEAHGALGLR
                     VERTEAFAPAFAQALAADRPALIEIVMDQEAITPRQSLSAIRAAALARQKG"
     gene            2197..3801
                     /locus_tag="P5558_19570"
     CDS             2197..3801
                     /locus_tag="P5558_19570"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRY26576.1"
                     /translation="MSTKPTSNDHGTGVRAAATAAAEGDRIIIFDTTLRDGEQSPGCS
                     MTTPEKIQVADLLESMGVDVIEAGFPIASQGDFEAVRAIAGRVKESVVCGLARAAIGD
                     IDRAAEALQPAARSRIHTFIGTSPLHRQFQLQLDQDQVHERVAASVTRARSYTDDVEW
                     SAMDASRTEHDFLFRCIETAIECGATTINIPDTVGYAYPEEFADLIRLIRSAVPNIDK
                     AVLSVHCHNDLGLAVANSLRAVQAGARQIECTINGIGERAGNCALEEVVMAVRTRPDQ
                     LPYASRIDTTKIMQASRLVSAITGFNVQPNKAIVGANAFAHESGIHQDGMLKNAQTYE
                     IMRPEDIGLVKSSLVMGKHSGRHAFKTKLKELGYELGDNALEDAFARFKALADKKKEI
                     FDEDLVALVDEEIATGDQTIRFVRLLVQCGSDIAPKAEMTLSIDGVEKSAAATGDGPV
                     DATFNAIKALYPHDVLLPLFQIHAVTGGTDAQAIVTVRLEEDGRTVNGQSADTDTLVA
                     SARAYIAALNKLLTKRGRHAPAALSA"
     gene            4019..5059
                     /locus_tag="P5558_19575"
     CDS             4019..5059
                     /locus_tag="P5558_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRY26577.1"
                     /translation="MFSRLLGLFSADLAIDLGTANTLVYMKGRGIVLNEPSVVAIATM
                     KGRKQVLAVGLEAKEMVGRTPGNIEAIRPLRDGVIADFEVAEEMIKHFIRKVNNRRGF
                     ASPQVIICVPSGSTAVERRAIQEAAEAAGARRVHLIEEPMAAAIGAGLPVTEPTGSMV
                     VDIGGGTTEVAILSLGGIVYARSIRVGGNKMDEAIINYVRRNHSLLIGEATAENIKKR
                     IGSACLPEDGNGEVMEIKGRDLMNGVPKEIVISQRQIAESLAEPVNAIIEAVKVALEH
                     TAPELSADIVDKGIVLTGGGSLLGNLDFVLRHSTGLPVSQADEPLNCVALGTGRCLED
                     MKGLKHVLHTAY"
     gene            5274..6185
                     /gene="mreC"
                     /locus_tag="P5558_19580"
     CDS             5274..6185
                     /gene="mreC"
                     /locus_tag="P5558_19580"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRY26578.1"
                     /translation="MLVAVIGVAVLLLVLVRFNVRVIEVVTDGIADRMVPAVALVREP
                     IALTRRIGDQIGALLAVRAENERLRAENRRLLEWQSRAVWLTVENRSLRTMLDMTTAT
                     PETALTTAQIVADSAGPFVHTRLIDAGRDHAIEVGMAVLTPQGMVGRIIDVGARSARV
                     LLLTDFNSKLPVIIERSGDQALLAGDNTAEPKLQFLPMNPRFAIGDRVMTSGKGGVLP
                     PGLMIGEIARIDGGRVAVRTFVEWPTVDFVSVLRHAPLPAPESDTDGGLEPTAPELEP
                     EPALADQGGADLRGIGQPGALVGLVGG"
     gene            6182..6733
                     /gene="mreD"
                     /locus_tag="P5558_19585"
     CDS             6182..6733
                     /gene="mreD"
                     /locus_tag="P5558_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRY26579.1"
                     /translation="MTGFGQGGIVASGRPGDLARAALPFLSVFVLICVDLAPLPSAAP
                     TTIAPLLSLAAIFYWTVQRPDLLGNGLTLILTLLLDAASGMPLGLTALALFATRLVLL
                     APRRFLSSRSFAVLWACFCLVAMLLLGLRWVIACLFWQEALPLRPVLFEILLTIAAYP
                     LVSLALAFLLPVLPKPSHAASRG"
     gene            6714..>7146
                     /locus_tag="P5558_19590"
     CDS             6714..>7146
                     /locus_tag="P5558_19590"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRY26580.1"
                     /translation="MQHHAADRTRTFTRRALVVGAAQVGLFGLLAGRLWQLQVDDASR
                     YALLAEENRVNQRLIVPPRGRIFDREGRVLARNVPSYRLRIVRENTPDLAKTLLDLAR
                     LIPLDQAQIDEILKTARAQRAFVPVLVREGLSWDEVTTVAVH"
BASE COUNT         1077 a         2546 c         2392 g         1131 t
ORIGIN      
        1 cgcggctcac cccgctcgac ttcgagtggt atctctcggc cgtctgagcg ctcagccttt
       61 ctgcctggcc agggcggcag cccggatcgc cgagagcgac tggcgcggcg tgatcgcctc
      121 ctgatccatg acgatctcga tgagcgccgg ccgatcggcg gcgagcgcct gtgcgaacgc
      181 cggggcgaac gcctcggtgc gctcgacccg gagccccagg gcaccgtggg cctcggcgag
      241 cctggcgaaa tcggggttgg cgagctccgt gccgtagacc cgcgtcgggt actcgcgttc
      301 ctggtgcatg cggatcgtgc cgtacatgcc gttgttgaag accaggatga cgatggcgag
      361 gcgctgctgg caggcggtcg ccagctcctg cccggtcatc aaaaagcagc cgtcgccggc
      421 gaagcagacg accggccggt cggggtgggc gagcttggcg gcgatcgccg ccggcaggcc
      481 gtagcccatc gagccggagg tcggggcgag ctgcgagcgg aaggcccggt agctgtagaa
      541 gcggttgcac caggccgagt agttgccggc accgttggtg acgatggcct cggccgggag
      601 ggtcgcacgc agctcggcca tgatcgccgg catctgcacg ttgcccggta tttccagcgg
      661 agcgatgttc tcgagatagg cggcatggcc gtcggcggtg ccgagcgccc aggtccggct
      721 ggcgagcggc ccgcgtgccg ccagggcctc gacgaaaggc ccggggctcg cctggatcag
      781 gaggtccggc tggtagaccc gaccgagctc ctccggcccg ttgagcacct ggatcatgcg
      841 ctgcttcggt cgtggcaggc cgagcctggt gtagccgtcg ctcgtcatct cgccgagccg
      901 ggcaccgatg accaggagga gatcggcatg gtcgagccgc tcgagcagct tcgggttggc
      961 gccgatgccg atgtcaccga catagcagcg atgcgtgttg tcgaggtagt cctggcggcg
     1021 gaagccgacg gcggtcggca ggtcgttcgc ctcgacgaac cggcgcaatg ccgtcaccgc
     1081 atcctggtcc cagccgccgc cgccgaggat gacgagcggc cgctcggcct cgccgagcca
     1141 ggtcatggtc gtctcgaccg ctgccgggtc gggatgggcc cgcacctgct gccagggccg
     1201 gccgggctcg gtcatcacct cgtcggtcag catgtcctcg ggcagggcca ggaccaccgg
     1261 ccccggccgg ccggtggtgg cggtcatgaa ggcgcggtgc agatattccg ggatccgccg
     1321 cgcatcctcg atctcggcga cccatttggc gatgccgccg aacatctgcc ggtagtcgac
     1381 ctcctggaag gcctcgcggt cgcgcgcctc gcgggcgatc tggccgacca gcaggatcat
     1441 cggtgtcgaa tcctggcggg cgatatggac cccggccgac gcattggtgg cacccggccc
     1501 gcgggtgacg aagcagacgc ccggccgccc ggtgagcttg ccatcggctt ccgccatcat
     1561 cgcggcacca ccctcctggc gacagatgac gaaggcgatg tcgttgcggc catgcagcgc
     1621 gtcgagcgct gccagatagc tttcgccggg cacgccgaag atccgatcgg tgccgtgccg
     1681 caccagctcg tcgatcagga tctcgccgcc ggagcgtctc gtcgtcgtca tccgttcctc
     1741 ctgtcgggtc gcggcccgtc ccagcctcgg cacttgcccg tccgcacgac ttgcccggat
     1801 cggcccgccg ccgcaagcct cggcaggtga tccacaccgc tgcccggcac aaccgacagg
     1861 gcgaccttcc ccggccgggt tcgctgatgg ggcgatcaag ctgctttaca attcggtaac
     1921 tttgacggca tatcctcggg actatcgaca ccatccaccc gggttgccgg ccaggatgat
     1981 gccggcaaag gtggtcatga cgatgcacgg gcacgacact cggggtcttc ggcaagcggc
     2041 aagcaccgct gtcgccggcc tgtgcgcgcc gctcgctgac cgggttctct cgctgcggct
     2101 tcgacttacc tgcccctgag cccgcccgtt cgaacacgcg tgcgctcagg ggtcgagttc
     2161 gaaccgcaac gtcagccacg cacgagagcc agcatcatga gcaccaagcc gacgagcaac
     2221 gaccacggca ctggcgtacg cgccgccgcc accgcggcgg ccgagggcga tcgcatcatc
     2281 atcttcgaca ccacgctgcg cgacggcgag cagtcgcccg gctgctcgat gaccacgccc
     2341 gagaagatcc aggtcgccga cctcctggag agcatgggag tcgatgtcat cgaggccggc
     2401 ttcccgatcg cctcgcaggg tgatttcgag gccgtccggg cgatcgccgg gcgagtcaag
     2461 gagagtgtcg tctgcggcct cgcccgcgct gccatcggcg atatcgaccg ggccgccgag
     2521 gcgctgcagc cggcggcgcg aagccgcatc cacaccttca tcggcacctc gccgctgcac
     2581 cgccagttcc agctgcagct ggaccaggat caggtgcacg agcgtgtcgc cgccagcgtc
     2641 acccgggcgc gctcctacac ggacgacgtc gagtggagtg cgatggatgc atcgcgcacc
     2701 gagcacgact tcctcttccg ctgcatcgag accgcgatcg agtgcggcgc cacgaccatc
     2761 aacatccccg acacggtggg ctacgcctat ccggaggagt tcgccgacct gatccgactg
     2821 atccggagcg ccgtgcccaa catcgacaag gcggttctct cggtccactg ccacaacgac
     2881 ctgggccttg ccgtcgccaa cagcctgcgc gccgtgcagg cgggtgcgcg gcagatcgag
     2941 tgcacgatca acggcatcgg cgagcgcgcc ggcaactgcg cgctcgagga ggtggtcatg
     3001 gcggtccgga cccgccccga ccagctgccc tacgcgagcc gcatcgacac caccaagatc
     3061 atgcaggcct cgcggctggt ctcggcgatc accggcttca acgtgcagcc gaacaaggcg
     3121 atcgtcggcg ccaacgcctt cgcccacgag agcggcatcc accaggacgg gatgctgaag
     3181 aatgcccaga cctacgagat catgcggccc gaggacatcg gcctcgtcaa gtcgagcctg
     3241 gtgatgggca agcattccgg ccggcacgcc ttcaagacca agctgaagga gctgggctac
     3301 gagctcggcg acaatgcgct cgaggatgcc ttcgcccgtt tcaaggccct ggccgacaag
     3361 aagaaggaga tcttcgacga ggacctggtg gcgctggtcg acgaggagat cgccacgggc
     3421 gaccagacca tccgcttcgt ccgcctgctc gtccagtgcg gctcggacat cgccccgaag
     3481 gccgagatga cgctgtccat cgacggtgtc gagaagtcgg cggcggcgac gggcgacggg
     3541 ccggtcgatg cgaccttcaa cgcgatcaag gctctctacc cgcacgacgt gctgctgccg
     3601 ctcttccaga tccacgcggt gaccggcggc accgatgccc aggcgatcgt cacggtgcgg
     3661 ctcgaggagg atggccgcac ggtcaacggc cagtcggccg acaccgacac gctggtcgcc
     3721 tcggcccgcg cctacatcgc ggccctgaac aagctcctga ccaagcgtgg tcgtcacgcg
     3781 ccggccgcgc tgtcggcgtg aggctggcgg gcttggctcg gctctccctg gggaccgcgg
     3841 cgcgccagcc cgccactcgc ctccgcgtca gtatcccgat catcgttgac gggggtgcct
     3901 cggcaccccc gttgcgtgtt tcgacccggg tcgcgttccc gccccccggg tcggcagggg
     3961 tgcggctcga gccgtgccca cctcccccat cgccgatcac ggagcggatc ccgcacccat
     4021 gttctcgcgc cttctcggtc tcttctcagc cgacctcgcc atcgatctcg gcacggccaa
     4081 cacgctcgtt tacatgaagg ggcgggggat cgtcctgaac gagccctcgg tcgtcgccat
     4141 cgccaccatg aaaggccgca agcaggtcct ggccgttggc ctcgaggcca aggagatggt
     4201 cggccgcacg cccggcaaca tcgaggcgat ccgccccttg cgcgacggcg tcatcgccga
     4261 tttcgaggtc gccgaggaga tgatcaagca cttcatccgc aaggtgaaca accggcgcgg
     4321 cttcgccagc ccccaggtca tcatctgcgt gccttcgggc tccaccgcgg tcgagcggcg
     4381 tgccatccag gaggcggccg aggcagcggg ggcccgacgc gtgcacctga tcgaggagcc
     4441 gatggccgcg gccatcgggg ccggcctgcc ggtcaccgag cccaccggct cgatggtcgt
     4501 cgatatcggc ggtggcacca ccgaggtggc gatcctgtcc ttgggcggca tcgtctatgc
     4561 ccgctcgatc cgggtcggcg gcaacaagat ggacgaggcg atcatcaact atgtgcggcg
     4621 caaccacagc ctgctgatcg gcgaggccac ggccgagaac atcaagaagc ggatcggctc
     4681 ggcctgcctg cccgaggacg gcaatggcga ggtcatggag atcaagggcc gcgacctgat
     4741 gaacggcgtc cccaaggaga tcgtcatcag ccagcggcag atcgccgaga gcctggccga
     4801 gccggtcaac gccatcatcg aggcggtcaa ggtggcgctc gagcacaccg ccccggagct
     4861 ttcggccgac atcgtcgaca agggcatcgt gctcaccggc ggcggctcgc tgctcggcaa
     4921 tctcgatttc gtcctgcgcc actcgaccgg cctgccggtg agccaggccg acgagccctt
     4981 gaactgcgtg gcgctcggca ccgggcgctg cctcgaggac atgaaggggc tcaagcacgt
     5041 cctgcacacg gcctattgag cagtcggccg atggtgccgt taaccctttg ttgccggtgg
     5101 gtgacatacc atcctcaggc ggcagatgac ccaacgcttg gttgccacca ggcgacgttg
     5161 ctcgtgctag tctgtcaccc gtcatcgcag cgtgcggcaa gacagtgaag atcgccttcg
     5221 tcgaccgatt cgtccaattc gggaccgcgt tgcgcgccgc tggcgagcga ccggtgctgg
     5281 tcgccgtcat cggggtcgcg gtgctgctcc tggtgctcgt ccggttcaat gtgcgcgtca
     5341 tcgaggtggt gaccgatggc attgccgatc gcatggtgcc ggcggtcgcc ctggtgcgcg
     5401 agccgatcgc cctcacccgc cggatcggcg atcagatcgg cgcgctcctc gccgtgcgcg
     5461 ccgagaacga gcggctgcgg gcagaaaatc gccgcctgct cgaatggcag tctcgcgcgg
     5521 tgtggctgac ggtcgagaac cgctcgctgc gcaccatgct cgacatgacc acggcgaccc
     5581 ccgagaccgc actgacgacg gcgcagatcg tcgccgattc cgccggcccg ttcgtccata
     5641 cccggctgat cgacgccggc cgcgaccacg ccatcgaggt cggcatggcg gtgctgacgc
     5701 cgcagggcat ggtcggccgg atcatcgatg tgggggcccg ttccgcccga gtgctgctgc
     5761 tcaccgattt caactcgaag ttgcccgtga tcatcgaacg gtcaggcgat caggcgctgc
     5821 tcgccggcga caacaccgcc gagcccaagc tgcagttcct gccgatgaac ccgcgcttcg
     5881 cgatcggcga ccgggtcatg acctcgggca agggcggggt cctgccgccg gggctgatga
     5941 tcggcgagat cgcccggatc gatggcggcc gggtcgccgt caggaccttc gtcgagtggc
     6001 cgacggtcga tttcgtctcg gtcctccggc acgccccgct gccagcgccc gaaagcgaca
     6061 cggatggcgg cctggagcca accgcgcccg agctcgagcc ggagccggcg ctggccgatc
     6121 aaggcggggc cgacctgcgg gggatcggcc agccgggggc gcttgtcggg ctggtcggcg
     6181 gatgacgggc ttcggccagg gcggcatcgt ggcgagcggt cgaccgggcg atctggcccg
     6241 cgccgccctc cccttcctct cggtcttcgt gctgatctgc gtcgacctgg cgcccctgcc
     6301 gagcgccgcc ccgacaacca tcgcaccgct gctcagcctc gccgcgatct tctactggac
     6361 ggtgcagcgc ccggacctgc tcggcaacgg cctcaccctc atcctcacct tgctgctcga
     6421 tgccgcttcc ggcatgccgc tcggtctcac cgccctcgcc ctcttcgcca cgcggctggt
     6481 gctgctggcc ccgcgccgat tcctctccag ccgctcgttc gccgtgctct gggcgtgctt
     6541 ctgtctcgtg gccatgctgc tgctcgggct gcgctgggtg atcgcctgcc tcttctggca
     6601 ggaggccctg ccgctgcgtc cggtgctgtt cgaaatcctg ctcaccatcg ccgcctatcc
     6661 cctggtgagc ctcgccctcg cctttctcct gcctgtcctg ccgaagccga gccatgcagc
     6721 atcacgcggc tgaccgcacc cgcaccttca cccgccgggc cctcgtggtc ggcgccgccc
     6781 aggtcggcct cttcggcctg ctggccgggc ggctctggca gctgcaggtg gacgacgcct
     6841 cacgctatgc tctgctcgcc gaggagaacc gggtcaacca gcggctgatc gtgccaccac
     6901 ggggccgcat cttcgatcgc gagggtcgcg tgctggcgcg caacgtgccg agctaccgcc
     6961 tgcgcatcgt ccgcgagaac acgccggatc tggccaaaac cctgctcgat ctcgcccgcc
     7021 tcattcctct cgaccaggcc cagatcgacg agatcctgaa gaccgcccgc gcccagcgcg
     7081 ccttcgtgcc cgtcctggtg cgcgaggggc tgagctggga cgaggtcacc accgtcgccg
     7141 tgcaca
//
LOCUS       DAOTFI010000238         7142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12526_length_7142_cov_6.694793, whole genome shotgun sequence.
ACCESSION   DAOTFI010000238 DAOTFI010000000
VERSION     DAOTFI010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12526_length_7142_cov_6.694793"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="P5558_19595"
     CDS             complement(<1..306)
                     /locus_tag="P5558_19595"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY26581.1"
                     /translation="MEETFDIVVVGGGGAGLMATLAAARLGRRVLLLEKNPALGGTTG
                     LSVGSICTTSTPHQRAMGIHDTPDAHFEDMEKFAGPLVARDNPVLRRLLVDEVPETFR
                     "
     gene            488..1678
                     /locus_tag="P5558_19600"
     CDS             488..1678
                     /locus_tag="P5558_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRY26582.1"
                     /translation="MLLDGMKIVSFCHFLQGPAAMQYLGDMGAEIIRIEPPKGAYERV
                     WAGAGKIRVGGVSAFYLCANRNARSLALDLKRPEAKEIVLRMLEKSHALAENFRPGTL
                     DRLGLGYDVVKARKPDIIYASASGFGATGPFVDRPGQDLLIQAMSGIASANGGGGHRP
                     TAIGAAAVDQHGGALFALGILGAYAKWLRTGEGTRVEASLLGAGIDMQMESITHYYAA
                     AKGDKVFDRHESLATWFHEAPYGIYRIADGWVALSLNPVEKLGRALGEEAFAPYVGRD
                     QYADRDAIAAAVAVAVRERSFADLAAAFDAEAMWYGRVDNFDDLAANPQVQHNQVFQD
                     CEVNGETIRLVNHPNRYDGAAPAAPTFALETGADTRDVLAEAGYAEAEIEELLKDQIV
                     HAPR"
     gene            1702..2145
                     /locus_tag="P5558_19605"
     CDS             1702..2145
                     /locus_tag="P5558_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRY26583.1"
                     /translation="MSDFYVKVGDAVEVAKTIGESDVYVYAGVTGDFSVNHMNEQFME
                     KTSYGHRIAHGALLVGFMSTASTKMIDHVQGIGDAETPVSLGYDRVRFIGPVFFGDTI
                     TVQYTITAIDPERRRSTSDIKVVNQKGETVAVAQHILKWVPNDRK"
     gene            2168..2911
                     /locus_tag="P5558_19610"
     CDS             2168..2911
                     /locus_tag="P5558_19610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY26584.1"
                     /translation="MIFDLSGKRVLVIGGSYGMGFATARAALELGATVAIAARGREKL
                     EEAAASLEQVAGEPVPWTALRIEDRPAVAAFIARHAPFDHLVLPGSTVKPILYQDLDD
                     ASAREAFESKFWGPFWACFDARPHLRRGGSIVLYSGVAAVRPVKGYLMGACINGAINA
                     ATRSLALDFAAQGVRVNTIAPGLTDTPLNDVLHAHDKKERYAALGSRLPVGRIGTADE
                     AAQAAIYLMCNGYVTAEVLGVDGGQQVMD"
     gene            2920..4515
                     /locus_tag="P5558_19615"
     CDS             2920..4515
                     /locus_tag="P5558_19615"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRY26585.1"
                     /translation="MVEFITAAEAAALVRPRDCVLIGGSGGGHAVPEAFIEALAAHYK
                     ESGSPRDLSLVSIVSIGDWQETGFNILAEPGLARRVVSGGFNNCPRFAAMAIADEIEA
                     YTLPQGALSQLCRDMAAGRPGLLSRTGLHTFIDPRHGGGRQSPRSTEDLVELVTVRGE
                     EYLLFRSFPVDVAVIRGTTADERGNISMEDEAFFGENFSIAGAAHLHGGIVIAQVKQV
                     AAAGTIPGKLVKVPGVVVDYVIVEPEQRQTYLTRQSAAYAGDLRLADRSMQPIPFDIR
                     KVIARRGAMELFPGAVVNLGFGVSNGISAVAVEEGFYREVTLTVEQGIIGGVPAVGKD
                     AGAGVNYDMMADQPYQFDFYDGGGLDIAYLSFAEVDAAGNVNVSRYGKSVNGPGGFIN
                     ISQGARKVVFSGTLTTGGLDMAPDGKGGLELRQDGRTQKWVPEVGQVTFNGAYARERG
                     QEVMYITDRAVFRLGEKGLRLEEVAPGIDVRADVLERIGFPVEVRSEPRRMDARLFRE
                     APMAMIDDFRRRQGGRRPRGEQA"
     gene            4512..5294
                     /locus_tag="P5558_19620"
     CDS             4512..5294
                     /locus_tag="P5558_19620"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRY26586.1"
                     /translation="MSEPVHCTIEDGVATVTLANPPLNLVTLGLTARLDALLDELAAD
                     SKARVLVLTGTGERAFCAGSDISEFSDLTEPGVVLEKKLNKENQAYSKVDDFPKPTIA
                     AVRGLAFGGGLELAVCCDIIVVEENVRLALPEIKLGVFPGSGGTVRVTRRIGEGRAKE
                     MMFTGEPIDAQTALAWGLVNRVVPKGEALAAAQALARQLAERPNRALQACKRAVDMSF
                     DMSEDEAIQETLALSEEVFTTEDCKEGVRAFFAKEAPRFTHK"
     gene            5308..5742
                     /locus_tag="P5558_19625"
     CDS             5308..5742
                     /locus_tag="P5558_19625"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRY26587.1"
                     /translation="MTRLLVLQGANMNWLGRRQPEIYGRTTAAELDTLLRAHAAEHGY
                     EIEIVYKNHEGDAIDALYAAEAGDVDAVVLNPGGFSYAGYALRDCIKGIELPVIEIHL
                     SNHYARNIHSVTAEAARGVFMGLGIGTYIAGLEAALRLARRG"
     gene            5754..6545
                     /locus_tag="P5558_19630"
     CDS             5754..6545
                     /locus_tag="P5558_19630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY26588.1"
                     /translation="MAELRLADGGHLHYELAGSGSPLLLVPGLGGLAEFWNPVLPALA
                     ERFTVILHDHRGCGRSSQERIDFSIGQMAGDVLALLDHLGLEKAHLVGHSTGGAVGQT
                     IALDHPGRLDRLVLSGTWAAPDAYFESLFATRSAILRAGGPALYLQSSALMLYPPWWI
                     RDHPALVTITEEAARARVPDPEILLARIAAILRHDRLADLHRVTAPTLAIGARDDMVA
                     PAHLSEALGRLVGGAETLILPDGGHFFPITRADRFVRAVAGFLEG"
     gene            complement(6542..>7142)
                     /locus_tag="P5558_19635"
     CDS             complement(6542..>7142)
                     /locus_tag="P5558_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY26589.1"
                     /translation="LITHGVRGLRFGDIADRLQITRANIHYHFGTKKNLVEEVIDEYV
                     QQALAGLGAVWEDPGLTYQEKTRRTMEFNRGRYVKFNPAGRSGRSWSLISRMRLEADQ
                     LSDEARARLRHFTLAIETFIDEAVVRAQKNGELSAVAPVKDIAIQLIAIVDSAGSITQ
                     DGGSFDRLEHLYTAYLHIIDHAYGTGRGAAKRPVLAAGS"
BASE COUNT         1118 a         2532 c         2410 g         1082 t
ORIGIN      
        1 gcggaaggtc tccggcacct cgtcgaccag caggcgccgg agcaccgggt tgtccctcgc
       61 cacgagcggg ccggcgaact tctccatgtc ctcgaagtgg gcgtccggcg tgtcgtggat
      121 gcccatggcc cgctggtgcg gggtcgaggt ggtgcagatc gagccgaccg agaggccagt
      181 ggtgccgccg agcgccgggt tcttctcgag cagcagcacc cggcggccga ggcgggccgc
      241 ggcgagcgtg gccatcaggc cagcgccgcc gccaccgacg acgacgatgt cgaacgtctc
      301 ctccatcccc gggctcccct cgctcacatc ctcggtcttg acgacagctc tatcaaatgg
      361 cttactgata tgtaagtagg gagcgatgag cgggctcgca tgaccggggc ggctcgaacg
      421 cacgatggcg cgtcttcccg gaaggcgcta tcgagcgggc gcaacctgag aacgggggag
      481 gccaggcgtg ctgctcgacg gcatgaagat cgtgagcttt tgccacttcc tgcaaggccc
      541 cgcggccatg cagtatctgg gcgacatggg ggcggagatc atccgcatcg agccgcccaa
      601 gggcgcctac gagcgggttt gggcgggtgc cggcaagatc agggtgggcg gggtgagcgc
      661 cttctatctc tgcgccaacc gcaatgcccg cagcctcgcc ctggacctga agcggcccga
      721 ggcgaaggag atcgtgctgc gcatgctcga gaagagccat gcgctcgccg agaactttcg
      781 ccccggcacc ctggaccggc tcggcctcgg ctacgacgtg gtcaaggccc ggaagcctga
      841 catcatctac gcctcggcct cgggcttcgg cgccaccggc ccctttgtcg accggccggg
      901 ccaggacctg ctcatccagg ccatgtccgg catcgcctcg gccaatggcg gcggcggcca
      961 tcggccgacc gcaatcggcg cggcggccgt cgaccagcat ggcggtgccc tcttcgcgct
     1021 cggcatcctc ggcgcctacg ccaagtggct gcgcaccggc gagggcacgc gcgtcgaggc
     1081 cagcctgctc ggcgccggca tcgacatgca gatggaatcc atcacccact attatgccgc
     1141 ggccaagggc gacaaggtct tcgaccggca cgagagcctc gccacctggt tccacgaagc
     1201 gccctacggc atctaccgga tcgccgatgg ctgggtcgcc ctgtcgctca acccggtgga
     1261 gaagctgggc cgcgcgctcg gggaggaggc cttcgcgccc tatgtcggcc gggaccagta
     1321 tgccgaccgc gacgccatcg ccgccgccgt cgccgtggcg gtccgcgagc gcagcttcgc
     1381 cgacctcgcc gccgccttcg atgccgaggc catgtggtac ggcagggtcg acaatttcga
     1441 tgacctcgcg gccaacccgc aggtccagca caaccaggtc ttccaggact gcgaggtcaa
     1501 tggcgagacc atccgcctcg tcaaccatcc caaccgctac gacggcgcgg caccggccgc
     1561 gcccaccttc gcgctggaga cgggggcgga cacccgcgac gtgctggccg aggccggcta
     1621 cgccgaggcg gagatcgagg agctcctgaa ggaccagatc gttcacgcgc cacgctaggt
     1681 gctaagagaa gaggcaagcc agtgtcggat ttctacgtca aggtcggcga cgcggtcgag
     1741 gtcgcgaaga cgatcggcga aagcgacgtc tacgtctatg cgggcgtcac gggggatttc
     1801 tcggtcaacc acatgaacga gcagttcatg gagaagacca gctatggcca ccggatcgct
     1861 catggcgcct tgctcgtcgg cttcatgtcg accgcgtcga ccaagatgat cgaccatgtt
     1921 cagggcatcg gcgatgcgga gacgccggtt tcgcttggct acgaccgggt ccgcttcatc
     1981 ggccccgtct tcttcggcga caccatcacc gtgcagtaca cgatcaccgc catcgacccc
     2041 gagcgccgtc gctcgaccag cgacatcaag gtggtcaacc agaagggcga gacggtggcg
     2101 gtcgcccagc acatcctcaa atgggtgccc aacgaccgca agtgaggact ggagccggag
     2161 gcagcgcatg atcttcgacc tgagcggcaa gcgggtgctc gtcatcggcg gctcctacgg
     2221 gatgggcttc gccaccgccc gtgcggcgct cgagctcggc gccacggtgg ccatcgcggc
     2281 gcgtggccgg gagaagctgg aggaggcggc agcctcgctc gagcaggtcg ccggcgagcc
     2341 cgtgccctgg acggcgctcc gcatcgagga ccggccggcg gtcgccgcgt tcatcgcgcg
     2401 gcatgccccc ttcgatcacc tggtgctgcc gggctctacc gtgaagccga tcctctacca
     2461 ggatctcgac gacgcgagcg ccagggaggc gttcgagagc aagttctggg ggccgttctg
     2521 ggcctgcttc gatgcgcgcc cgcatcttcg caggggcggg agcatcgtgc tctacagcgg
     2581 cgtcgccgcg gtccggccgg tcaagggcta cctgatgggt gcctgcatca acggggcgat
     2641 caacgccgcc acccgctcgc tggcgctcga cttcgccgcc cagggggtgc gggtcaacac
     2701 catcgcgcct gggctgacgg acacgccgct caacgatgtg ctgcacgccc acgacaagaa
     2761 ggagcgctac gcggcgctcg gcagccggct gccggtcggc cgcatcggca ccgccgacga
     2821 ggccgcccag gcggcgatct atctcatgtg caacggctac gtgacggccg aggtcctcgg
     2881 cgtcgacggc ggccagcagg tcatggacta ggagcacgca tggtcgagtt catcacggcc
     2941 gccgaggccg cggccctggt caggccccgg gactgcgtcc tgatcggtgg ctcgggtggc
     3001 ggccatgccg tgcccgaggc cttcatcgag gcgctggccg cccactacaa ggagagcggc
     3061 agcccacgtg atctctcgct ggtgagcatc gtctcgatcg gcgactggca ggagaccggc
     3121 ttcaacatcc tggccgagcc cggcctcgcc cggcgggtgg tcagtggcgg cttcaacaac
     3181 tgcccgcgct tcgccgccat ggcgatcgcc gacgagatcg aggcctacac cctgccgcag
     3241 ggcgccctct cccagctctg ccgcgacatg gcggccggcc gccccggcct cctctcccgc
     3301 accgggctcc acaccttcat cgatccccgc cacggtggcg gccggcagag cccgcgctcg
     3361 accgaggacc tggtcgagct cgtcaccgtc cggggcgagg agtacctgct cttccgcagc
     3421 tttccggtcg atgtcgcggt catccgcggc accacggccg acgagcgcgg caacatctcg
     3481 atggaggacg aggcgttctt cggcgagaac ttctcgatcg ccggtgctgc ccacctgcat
     3541 ggcggcatcg tcatcgccca ggtcaaacag gtggcggccg ccggcaccat cccgggcaag
     3601 ctggtcaagg tgccgggtgt cgtcgtcgac tacgtcatcg tcgagcccga gcagcgccag
     3661 acctacctga cccgccagag cgcggcctat gccggcgatc tgcgcctcgc cgaccgcagc
     3721 atgcagccga tccccttcga catccgcaag gtgatcgcgc ggcgcggggc catggagctc
     3781 ttccccggtg ccgtggtcaa tctcggcttc ggcgtgtcca acggaatctc ggcggtagcg
     3841 gtcgaggagg gcttctaccg cgaggtcacg ctcaccgtgg agcagggcat catcggcggc
     3901 gtgccggcgg tcggcaagga tgccggggcc ggggtcaact acgacatgat ggccgaccag
     3961 ccctaccagt tcgacttcta cgacggcggc gggctcgaca tcgcctatct ctccttcgcc
     4021 gaggtggatg cggcgggcaa cgtcaatgtc agccgctacg gcaagtcggt gaacggcccg
     4081 ggcggcttca tcaacatcag ccagggggcg aggaaggtcg tcttctccgg cacgctgacc
     4141 acgggcgggc tcgacatggc gcccgacggc aagggcgggc tcgagcttcg ccaggacggc
     4201 cgcacccaaa aatgggtccc cgaggtcggc caggtgacct tcaatggcgc ctatgcccgc
     4261 gaacgcggcc aggaggtcat gtacatcacc gaccgcgccg tatttcggct cggcgagaaa
     4321 gggctccggc tcgaggaggt ggcacccggc atcgacgtgc gcgccgatgt gctggagcgc
     4381 atcggctttc cggtcgaggt gcggagcgag ccgcgccgca tggacgcaag gctcttccgc
     4441 gaggccccca tggccatgat cgatgatttc cgccgacgcc agggcgggcg tcgacccaga
     4501 ggagagcaag catgagcgaa cccgtccact gcacgatcga ggatggcgtg gccaccgtca
     4561 cccttgccaa cccgccgctc aacctcgtca ccctcggcct caccgcccgg ctggatgccc
     4621 tgctcgacga gctggcggcc gattcaaagg cgcgcgttct ggtcctcacc ggcacgggcg
     4681 agcgggcctt ctgcgccggc tccgacatct cggagttctc ggatctgacc gagcccggtg
     4741 tggtgctcga gaagaagctg aacaaggaga atcaggccta cagcaaggtc gacgacttcc
     4801 ccaagcccac cattgccgcc gtccgcggcc tcgccttcgg cggcgggctc gagctggcgg
     4861 tctgctgcga catcatcgtc gtcgaggaga acgtccgcct ggccctcccc gagatcaagc
     4921 tcggcgtctt cccgggcagc ggcggcacgg tgcgcgtcac gcgccggatc ggcgaggggc
     4981 gggccaagga gatgatgttc acaggcgagc ccatcgacgc gcagacggcg ctcgcctggg
     5041 gcctcgtcaa ccgcgtcgtg ccgaagggcg aggccctggc cgccgcccag gcgctcgccc
     5101 gacagctcgc cgagcggccc aaccgggcgc tccaggcctg caagcgcgcc gtcgacatgt
     5161 ccttcgacat gagcgaggac gaagcgatcc aggagacgct ggcgctcagc gaggaggtct
     5221 tcaccaccga agactgcaag gagggcgtgc gcgccttctt cgccaaggaa gcgcctcgct
     5281 tcacccacaa gtgaggaggc tggaccgatg accaggctcc tggtgctgca gggtgcgaac
     5341 atgaactggc tgggccgtcg ccagccggag atctacgggc ggacgaccgc cgccgagctc
     5401 gacacgctgc tccgggcgca tgccgccgag cacggctacg agatcgagat cgtctacaag
     5461 aaccacgagg gcgacgcgat cgacgcgctc tatgccgccg aggccggcga tgtcgacgcc
     5521 gtcgtcctca acccgggcgg cttctcttac gcgggctacg cgctccgcga ctgcatcaag
     5581 ggcatcgagc tgccggtgat cgagatccac ctctcgaacc actacgcccg caacatccac
     5641 tcggtcaccg ccgaggcggc caggggcgtc ttcatggggc tcggcatcgg cacctacatc
     5701 gccggcctcg aggcggcgct ccgcctcgcg cgccggggct gatcgaggag cccgtggccg
     5761 agctgcgcct cgccgatggc gggcacctgc actacgagct cgccggcagc ggctcgccgc
     5821 tcctcctggt ccccgggctc ggcggcctcg ccgagttctg gaaccccgtc ctgccggcgc
     5881 tggccgagcg cttcaccgtc atcctccacg accatcgggg ctgcggccgg agcagccagg
     5941 aacggatcga cttctcgatt ggccagatgg ccggcgacgt cctggccctg ctcgaccatc
     6001 tgggcctgga gaaggcccat ctcgtcggcc actccaccgg cggggccgtc ggccagacga
     6061 tcgccctcga ccatcccggc cgcctcgacc ggctggtgct gagcggcacc tgggccgccc
     6121 cggatgccta tttcgagagc ctcttcgcca cgcgcagcgc catcctgcgg gcgggcggcc
     6181 ccgccctcta tctgcagagc tcggccttga tgctgtatcc gccctggtgg atccgcgacc
     6241 acccggcgct cgtgaccatc accgaagagg cggcccgggc gcgcgtgccc gatccggaga
     6301 tcctgctcgc ccggatcgcc gccatcctgc gccacgaccg gttggccgat ctccaccgcg
     6361 tcaccgcgcc gacgctggcc attggtgccc gcgacgacat ggtggccccg gcccacctct
     6421 cggaggcgct cggccggctc gtcggcggtg ccgagaccct gatcctgccg gacggcggcc
     6481 acttcttccc gatcacccgg gcggaccggt tcgtccgcgc cgtcgccgga ttcctcgagg
     6541 gctaggagcc ggcggccagg acgggccgct tcgccgctcc ccggcctgtg ccgtaggcat
     6601 ggtcgatgat gtgcagatag gccgtgtaga ggtgctctag ccggtcgaac gagccgccgt
     6661 cctgggtgat cgagccggcg ctgtcgacga tcgcgatgag ctggatggcg atgtccttga
     6721 ccggggccac agcgctcagc tcgccgttct tctgggcgcg caccaccgcc tcgtcgatga
     6781 aggtctcgat cgccagcgtg aaatggcgga gcctcgcccg ggcctcgtcg ctgagctggt
     6841 cggcctcgag ccgcatcctg gagatcaggc tccacgatcg ccccgaccga cccgccggat
     6901 tgaacttcac atagcgcccg cggttgaact ccatcgtccg ccgcgtcttc tcctggtagg
     6961 tgagcccggg atcctcccaa accgcgccga gcccggcgag cgcctgctgc acgtactcgt
     7021 cgatcacctc ctcgacgaga ttcttcttgg tgccgaagtg atagtggata ttggcgcggg
     7081 tgatctggag ccgatcggcg atgtcgccga agcgcaggcc gcgcacgcca tgggtgatca
     7141 ga
//
LOCUS       DAOTFI010000239         7094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12624_length_7094_cov_7.249467, whole genome shotgun sequence.
ACCESSION   DAOTFI010000239 DAOTFI010000000
VERSION     DAOTFI010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12624_length_7094_cov_7.249467"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..902
                     /locus_tag="P5558_19640"
     CDS             282..902
                     /locus_tag="P5558_19640"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRY26590.1"
                     /translation="MATSDEIRLDVDKVEEAGARSHALAFPPNAVSGVLERLVERVGK
                     ACSWIWVILIVLIVVNVALRYVVGTNFIAMEELQWHLYAIGFLMALSFAVLVDGHVRV
                     DVIAEHMPLRRRAWIELFGLLFLLLPFVVVVTYNAVPFVERAYQIGEVSAAPGGLPMR
                     WIIKSFMITGFVFVAIAAMARLLRVTALLFRIPRPHGDDRVREDAS"
     gene            902..2263
                     /locus_tag="P5558_19645"
     CDS             902..2263
                     /locus_tag="P5558_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY26591.1"
                     /translation="MLATYEILVIVMLGSFIALIFTGFPIAWILGGLAVVFAVVGVLG
                     AEFLDLDTYFLTDWRIFGVIVQRIYAQMANWVLVALPMFIFMGLMLERSGVAERLMVD
                     FSRLFGRFPGGLGLAVVVIGILLAASTGIVGASVVLLAMLSVPIMLDQRYSPAFAAGV
                     VASSGTLGILIPPSIMLIIMADQLSLSVGNLFMGALIPGLLLGGLYILFVLVAARLVP
                     GLAPAPRDLPKVTGRLVLTTLLHVIPPLLLILAVLGSIFFGIATPTEASGLGAFGATL
                     LALANGKLSFEVVKEVCYKTSITTAFIFGIFLGATAFSVVLRQLGGDEFIAHVLHALP
                     LGPGGIVLAILVIAFVAGFFLDWLEITLIMLPLVSPVVEALGFDLTWFVVLFAVTLQT
                     SFLTPPVGFSLFYLKGVVPPEVTTKMIYRGVVPFILLQLIGVALVYLWPSLVLWLPEV
                     AYG"
     gene            complement(2193..3131)
                     /locus_tag="P5558_19650"
     CDS             complement(2193..3131)
                     /locus_tag="P5558_19650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26592.1"
                     /translation="MVEIPRPTLRAAGWMLGSMISFSVMAVAARELSAAMSPFQILLL
                     RNLFGVAFIGLFIARADPAVLRSCQPVMQLVRHSVHFIGQVLWILAIAWLPLAIVFAV
                     EFTSPIWGALTAVLLLGERLSRNRQLAMALGFVGVLIIVRPGVGAFDPAIFAVVAAAL
                     SFGITNAMTKKLLATDRPWGILFWMCLVQTLLCLPFAIPLWTPLTWVQSPAIAGVAVA
                     GLSAHYCLNRALAAADAVVVLPMDYARLPMIAVVGLVLYGEQIVPWTFIGAAVVFLGI
                     WLNLRGDAARAQVLSRRRPRAATAPGSARGRPGPRR"
     gene            complement(3161..4375)
                     /locus_tag="P5558_19655"
     CDS             complement(3161..4375)
                     /locus_tag="P5558_19655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY26593.1"
                     /translation="MSISAIRPGILQVVPSLEEGTAARAILATAKLLAAEGWRSCVAS
                     AGGPLVRELVAHGSAHVTASIESSNPLTLSTNLAKLVKAIREHKVELVHAHSRAAAWS
                     ASFAARRAGAAFVTSFETAYAGGEGGLKKRYNAVLAQGARIIANSEYLAEHISKTYDV
                     EPARLRIVRQGIDLEAYDPAKMRGHRMAKLHERWQLGLERRIVLLPGRVVRGRGHLAM
                     LDAMQRLARDDFVLLILGDLDPADAYVRELESRVRSSGLAKRVHFASGGDDLPAAYAL
                     ADLVVMPAAADATDVDAVPAIEAQAMGKPVILGNVGALGEAVLPAATGWLVAPGDIDE
                     LAWSLDRALAMEKPTLERLAERARSFVMREYSTEQSARRTIAVYRELVRNAPRPNLPE
                     PVVERLAIPQVD"
     gene            4492..5241
                     /locus_tag="P5558_19660"
     CDS             4492..5241
                     /locus_tag="P5558_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY26594.1"
                     /translation="MQRLELGAAGSLAFCRTPGIGPEVLFLGGFASDMTGTKARHLEA
                     HCKSAGRAFTRFDYRGHGASSGRFEDGTIGDWAADATAVLDRVVEGPAVLVGSSMGGW
                     LMLLLALARPERVKGLVGIASAPDFTERLLRPGLSAAALAELRHHGLHRRPSTYGDPL
                     PITARLLDEGREHLLLEAPIAIGCPVHLLHGQADPDVPWQLGLELAGRLASRQVTLEL
                     IKDGDHRLSRETDLRRIALALDRVLEAASQT"
     gene            5269..5889
                     /locus_tag="P5558_19665"
     CDS             5269..5889
                     /locus_tag="P5558_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRY26595.1"
                     /translation="MTDELVFYTNPMSRGRIVRWMLEEIGEPYRTEIVAYGPKMRSPD
                     YLALNPMGKVPMVRRGKSVVTEAAAICAWLADVFPAAGLAPAPAERAAYYRWLFFGAG
                     PVEAAGSATSLGFEPKSPDQQRQVGFGSLERVTDTLERAVGEAPYLAGDRFSAADVYL
                     GSLIGWGYEFGPMEPRPALRHYVDRIRDREAYRRARAIDDAAAPSA"
     gene            complement(5881..6759)
                     /locus_tag="P5558_19670"
     CDS             complement(5881..6759)
                     /locus_tag="P5558_19670"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY26596.1"
                     /translation="MAEIARTLLCFGLGYSAEVLAQRLMGQGWRIVGTSRKAGKRREL
                     EARGYTMHAFERGTPLPASAFDGVTHVLTSIAPGEAGDPVLDMHQAELEGCGLAWAGY
                     LGTTGVYGDRGGAWVDETTPIEPTLARADRRARAEAQWLESGLPAHVFRLAGIYGPGR
                     NPFVNLRAGTARRIVKPGQVFSRIHVEDIATVLEASIARPNPGQVYNVCDDEPAPPQD
                     VVAHAAELMGVDPPPEQDFATAELSPMARTFYEDNRRVRNARIKLELGVRLAFPSYRE
                     GLLALLAVDGRPPASG"
     gene            complement(6747..>7094)
                     /locus_tag="P5558_19675"
     CDS             complement(6747..>7094)
                     /locus_tag="P5558_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY26597.1"
                     /translation="AQEAKLQARDELVAPSLERLLALDDGAFRQLFSGSPVKRIGRDR
                     FLRNVLIAAGNSGDAGLVPRLEALLGDASPLVRGAAVWALRRLATEAVVEPQRIAHLA
                     SEADPEVRDEWLR"
BASE COUNT          974 a         2508 c         2475 g         1137 t
ORIGIN      
        1 atcctgaacg ccttcgagga cgcgaccgcg accgtcatga agcgccggtc cgagcaggac
       61 gcggatttcg gccgggtcta cgcctcgatg cgcgccttcc aggaggagct caacccgtgg
      121 cggcagaagg gctacctgcc gcgcgactgg gattatcgct tcgaggagtg aggcggccgg
      181 ctgcgaccgt gacgcagcgg agcggccaag ggcccgctcc gctgccgcgg tccgcggcgg
      241 gcgtccgttg aagaaacctc gcacaacata cggctagcgc catggccacg agcgacgaga
      301 ttcgactcga tgtcgacaag gtcgaggagg cgggtgcgcg cagccacgct ctggcgtttc
      361 cgcccaatgc cgtgtccggc gtgctcgagc ggctggtcga gcgggtcggc aaggcctgct
      421 cctggatctg ggtgatcctc atcgtgctga tcgtcgtcaa cgtggccctg cgctatgtgg
      481 tcggcaccaa cttcatcgcg atggaggagc tgcagtggca tctctacgcc atcggcttcc
      541 tcatggcgct gagctttgcc gtgctcgtcg acggccatgt ccgggtcgac gtcatcgccg
      601 agcacatgcc gttgcgccgc cgggcctgga tcgagctttt cggccttctt ttcctgctgc
      661 tgccgttcgt cgtcgtcgtc acctacaacg ccgtcccgtt cgtcgagcgg gcctaccaga
      721 tcggcgaggt ctcggcggcg ccgggcgggc tgccgatgcg ctggatcatc aagtccttca
      781 tgatcacggg cttcgtcttc gtcgcaatcg cggccatggc gcgcctcctg cgcgtgaccg
      841 ccctgctttt ccgcatcccg cggccccacg gtgacgaccg ggtccgggag gacgcttcct
      901 gatgctcgcg acgtacgaga tcctcgtcat cgtcatgctg ggctcgttca tcgccctcat
      961 cttcacgggc ttcccgatcg cctggatcct gggcggcctc gccgtcgtct tcgccgtcgt
     1021 cggcgtgctc ggcgccgagt tcctcgacct cgacacgtac ttcctcaccg actggcggat
     1081 cttcggcgtc atcgtccagc gcatctatgc ccagatggcg aactgggtcc tggtcgccct
     1141 gccgatgttc atcttcatgg ggctgatgct cgagcgctcg ggtgtcgccg agcggctgat
     1201 ggtcgatttc tcgcggctct tcggccgctt ccccggcggg ctcggcctcg ccgtcgtggt
     1261 catcggcatc ctgctcgcgg cctccaccgg catcgtcggc gcctcggtcg tgctgctcgc
     1321 catgctgtcg gtgcccatca tgctcgacca gcgctacagc ccggccttcg ccgccggggt
     1381 ggtcgcctcg tccggcacgc tcggcatcct gatcccgccc tcgatcatgc tcatcatcat
     1441 ggccgaccag ctctcgctct cggtcggcaa cctgttcatg ggcgcgctga ttccggggct
     1501 cctgctcggc ggcctctata ttctcttcgt gctggtcgcc gcccgcctcg tgcccggcct
     1561 cgccccggcg ccacgcgacc tgccgaaggt cacgggtcgg ctcgtcctga ccacgctcct
     1621 gcacgtcatc ccgcccttgc tcctgattct cgccgtgctc ggctcgatct tcttcggcat
     1681 cgccacgccg acagaggcct cgggcctcgg cgccttcggc gccacactcc tcgccctcgc
     1741 caatggcaag ctcagcttcg aggtggtcaa ggaggtctgt tacaagacga gcatcaccac
     1801 ggccttcatc ttcggcatct tcctcggcgc taccgccttt tccgtggtgc tgcgccagct
     1861 cggcggcgac gagttcatcg cccacgtgct gcacgcgctg ccgctcgggc cgggcggcat
     1921 cgtcctcgcc atcctcgtca tcgccttcgt cgccggcttc ttcctcgact ggctcgagat
     1981 cacgctgatc atgctgccct tggtgtcgcc ggtggtcgaa gcgctcggct tcgacctcac
     2041 ctggttcgtc gtgctcttcg cggtcaccct gcagacctcg ttcctcaccc cgccggtcgg
     2101 cttctcgctc ttctacctca agggggtggt cccgcccgag gtcaccacga agatgatcta
     2161 tcggggggtc gtgcccttca tcctgctgca gctcatcggc gtggccctgg tctacctctg
     2221 gccgagcctg gtgctgtggc tgcccgaggt cgcctacggc tgagcacctg ggcgcgggcc
     2281 gcgtcgccgc gcaggttgag ccagatgccg agaaagacga ccgcggcccc gatgaaggtc
     2341 caggggacga tctgctcgcc gtagaggacg aggccgacca ccgcgatcat cggcagccgg
     2401 gcgtagtcca tcggcagcac gaccacggca tccgcggcgg cgagcgcccg gttcaggcag
     2461 tagtgggcgc tgagcccggc gacggcgaca ccggcgatcg ccggcgactg gacccaggtg
     2521 agcggcgtcc agagcgggat cgcaaacggc aggcagagca gggtctggac caggcacatc
     2581 cagaagagaa tgccccaggg ccggtcggtg gcgagcagct tcttggtcat ggcattggtg
     2641 atgccgaagg agagggcggc cgccacgacg gcgaagatcg ccgggtcgaa cgcgccgacg
     2701 cccgggcgga cgatgatcag caccccgacg aagcccagcg ccatggcgag ctgccggttc
     2761 cggctcagcc gttcgccgag cagcagcacc gcggtgagcg caccccagat gggcgaggtg
     2821 aactcgacgg cgaagacgat ggcgagcggc agccaggcga tggcgaggat ccagagcacc
     2881 tggccgatga aatgcaccga atggcgcacc agctgcatga ccggctggca ggagcgcagg
     2941 acggccgggt cggcgcgggc gatgaagagc ccgatgaagg cgacgccgaa gaggttgcgc
     3001 aggagcagga tctgaaacgg gctcatggcg gccgacagct cgcgcgcggc caccgccatg
     3061 acgctgaacg agatcatcga gccgagcatc cagccggcgg cgcgcagggt cggtcgcggg
     3121 atctccacca tgcaatgggc ccggtcgcgc cggcggccgg tcagtccacc tggggaatgg
     3181 cgagtcgctc gaccacgggc tcgggcaggt tcgggcgcgg ggcgttgcgg accagctcgc
     3241 gatagacggc gatggtgcgc cgcgcactct gctcggtgct gtactcgcgc atgacgaagg
     3301 agcgggcgcg ctcggcgagg cgctccaggg tcggcttctc catggcgagc gcccggtcga
     3361 gcgaccaggc cagctcgtcg atgtcgcccg gggccaccag ccacccggtg gcggccggca
     3421 gcacggcctc gcccagcgca ccgacattgc cgaggatcac gggcttgccc atggcctggg
     3481 cctcgatcgc cgggaccgcg tcgacatcgg tggcgtcggc ggcggccggc atgacgacga
     3541 gatcggcgag cgcataggcc gccggcaggt cgtcgccgcc gctggcgaaa tgcacccgtt
     3601 tggcgaggcc cgagctgcgc acccggcttt ccagctcgcg cacataggcg tcggcgggat
     3661 cgaggtcgcc caggatcagc agcacgaaat cgtcacgggc gaggcgctgc atggcgtcga
     3721 gcatcgccag atgcccgcgg ccgcggacga cccggccggg caggaggacg atccgccgct
     3781 cgagcccgag ctgccagcgc tcgtgaagct tcgccatgcg atggccgcgc atcttcgccg
     3841 ggtcgtaggc ctcgaggtcg atgccctggc ggacgatgcg cagccgcgcg ggttcgacgt
     3901 cgtaggtctt gctgatgtgc tcggcgaggt attccgagtt ggcgatgatc ctggcgccct
     3961 gggcaaggac cgcgttgtag cgcttcttga ggccgccctc gccgcccgca taggcggtct
     4021 cgaagctggt gacgaaggcc gcacccgccc gcctggcggc gaagctggcg ctccaggccg
     4081 cggcgcggct gtgggcgtgg accagctcga ccttgtgctc gcggatcgcc ttgaccagct
     4141 tcgccaggtt ggtcgagagg gtgagcgggt tgctgctctc gatcgaggcg gtgacatggg
     4201 cgctgccgtg ggcgaccagc tcgcgcacca atgggccgcc ggccgaggcg acgcaggacc
     4261 gccagccttc ggccgcaagc agcttcgcgg tcgccaggat ggcccgggcg gccgtgcctt
     4321 cctcgaggct cggcaccact tgcaggatgc ctggtcgaat ggcgctgatg ctcaaggtcg
     4381 ctcgccccgg gctggtggcg ccgcccgcga ccggggtggg gcagctcgac gggttctgct
     4441 agcagatgtc cggttggcgc aaaagtgcag gcgtgcaatc aggaggtgaa gatgcagcga
     4501 ctggagctgg gcgcggcggg ttccctcgcc ttctgccgga cccccggcat cgggcccgag
     4561 gtgctctttc tcggcggctt cgcctcggac atgaccggga ccaaggcccg ccacctcgag
     4621 gcgcattgca agagcgccgg ccgcgccttc acccgcttcg actaccgtgg ccatggcgct
     4681 tcctccggcc ggttcgagga cggcacgatc ggcgactggg cggcggatgc gacggccgtg
     4741 ctcgaccggg tggtcgaggg gccggcggtg ctggtcggct cgagcatggg cggctggctg
     4801 atgctgctcc tggccctggc ccggccggag cgcgtcaagg gcctcgtcgg catcgcctcg
     4861 gcccccgatt tcaccgagcg cctgctgcgc cccggtctct cggccgcggc cctggccgag
     4921 ctgcgccacc acggtctcca ccggcgccca tcgacctatg gcgaccccct gccgatcacc
     4981 gcccggctgc tcgacgaggg ccgcgagcac ctcctgctcg aggcaccgat cgccatcggt
     5041 tgcccggtcc acctgctgca cggccaggcg gacccggacg tgccctggca gctcggcctc
     5101 gagctcgccg gccggctggc gagccggcag gtgacgctcg agctgatcaa ggacggcgac
     5161 catcgcctgt cgcgcgagac cgacctgcga cgcatcgccc tcgccctcga ccgcgtgctc
     5221 gaggcggcga gccagacatg acccacagtg gcgcgaggtg acccgaccat gaccgacgag
     5281 ctggtcttct acaccaaccc catgtcccgc ggccgcatcg tgcggtggat gctcgaggag
     5341 atcggcgagc cctaccgcac ggagatcgtc gcctacggcc cgaagatgcg ctcgcccgac
     5401 tatctggcgc tcaacccgat gggcaaggtg ccgatggttc gtcgcggcaa gtctgtcgtc
     5461 accgaggcgg cggcgatctg tgcgtggctc gccgatgtct ttcccgctgc cggactcgct
     5521 cccgcgcccg ctgagcgggc cgcctattac cgctggctct tcttcggcgc cggcccggtc
     5581 gaggcggccg gctccgccac gtcgctgggc ttcgagccca agagccccga ccagcagcgc
     5641 caggtcggct tcggcagcct ggagcgggta accgacacgc tggagcgggc agtgggcgag
     5701 gcaccctacc tcgccggcga ccggttctcg gcggccgacg tctatctcgg ctccctgatc
     5761 ggctgggggt acgagttcgg cccgatggag ccgcgcccgg ctctgcggca ctatgtcgat
     5821 cgcatccgcg accgcgaggc ctaccggcgg gccagggcga tcgacgacgc ggcggcgccc
     5881 tcagcctgag gcgggcggcc tgccgtcgac cgcgagcagc gccaggaggc cctcgcggta
     5941 gctgggaaag gcgagccgca cgccgagctc gagcttgatc cgggcattcc gcacgcgccg
     6001 gttgtcctcg tagaacgtcc gagccatggg cgagagctcg gccgtggcga aatcctgctc
     6061 cggcggcgga tcgacgccca tgagctctgc cgcgtgcgcc accacgtcct gcggcggcgc
     6121 cggctcgtcg tcgcagacat tgtagacctg gcccggattc ggccgggcga tcgaggcttc
     6181 cagcactgtc gcgatgtcct cgacgtggat gcgcgagaag acctggccgg gcttgacgat
     6241 gcgccgggcg gtgcccgccc tcaggttgac gaaggggttg cggcccgggc cgtagatgcc
     6301 ggcgaggcga aagacatgcg ccggcagacc gctttccagc cattgcgcct cggcccgcgc
     6361 ccggcggtcg gcgcgggcga gcgtcggctc gatcggcgtc gtctcgtcga cccatgcgcc
     6421 gccccggtcg ccatagacgc ccgtggtgcc gagatagccg gcccaggcga ggccgcagcc
     6481 ttcgagctcg gcctggtgca tgtccagcac cgggtcgccc gcctcgcccg gggcgatcga
     6541 ggtgagcaca tgggtcaccc cgtcgaaggc cgaagcgggc aggggcgtgc cgcgctcgaa
     6601 ggcgtgcatg gtgtaccccc tggcttccag ctcgcggcgc ttcccggcct tgcgcgaggt
     6661 gccgacgatc cgccagccct ggcccatgag ccgctgggcc agcacctcgg ccgagtagcc
     6721 gaggccgaag cagagcaaag tgcgggctat ctcagccatt cgtctcggac ctccggatcg
     6781 gcttcgctgg cgagatgggc gatgcgctgt ggctcgacca cggcctccgt ggccagccgg
     6841 cggagcgccc agacggcggc accgcgcacc agcggcgagg catcgccgag gagtgcctcg
     6901 agccgcggca cgagcccggc atcgccgctg ttgccggcgg cgatcaggac attgcgcagg
     6961 aaccggtcgc ggccgatccg cttgacgggc gagccggaga agagctggcg aaaggcgccg
     7021 tcgtcgaggg ccagaagccg ctccagggaa ggtgccacca gctcgtcgcg cgcctgcagc
     7081 ttcgcctcct gcgc
//
LOCUS       DAOTFI010000240         7065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12679_length_7065_cov_6.520114, whole genome shotgun sequence.
ACCESSION   DAOTFI010000240 DAOTFI010000000
VERSION     DAOTFI010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12679_length_7065_cov_6.520114"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..262)
                     /locus_tag="P5558_19680"
     CDS             complement(20..262)
                     /locus_tag="P5558_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26598.1"
                     /translation="MRREPETQVQERLAEVATRARPPGGPAMPEIDCEFFDESDDALG
                     DLTPNLAGLAAGADDSDSWQRLGDVIGRLGFDLDVD"
     regulatory      391..503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            557..1294
                     /locus_tag="P5558_19685"
     CDS             557..1294
                     /locus_tag="P5558_19685"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRY26599.1"
                     /translation="MTDHDDRSRLARDAARVLARLRAERPRVHCLTNFVAMQLTANLL
                     LAAGAVPSMTMDAREMPAFVETSRALLVNLGMLDPWREAAIPVAIEAAHGLGRPWVLD
                     PVKVDRAPARRAFARRLLERAPAVLRCNAAEAEMLEPGPGIVTAVTGAADRISGGGRA
                     IELAGGTMLMDRVTAMGCAASALVAACLAVEPDPFLATVSGLLVMKVAGAIAAESAAG
                     PGSFVPLFLDAVHGLDAATLQRRAELA"
     gene            1291..1968
                     /gene="thiE"
                     /locus_tag="P5558_19690"
     CDS             1291..1968
                     /gene="thiE"
                     /locus_tag="P5558_19690"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRY26600.1"
                     /translation="MNVPIDIRRALALYAVLDPARCRGRDPVELVAAAASGGATLVQL
                     RDKASGTRDLVALAQAVMAALEPHAVPLIVNDRVDVALAAGAAGVHLGLSDMDPADAR
                     RLVGPDVILGITVHHGAEAGAVPAGLADYAGLGPVFATASKDPGDPPLGPKRLARLIG
                     EVRGHHQGLPACGIAGIDHTNAASVIGAGADGVAVISDLFMADDVEAAARRLRRIVDA
                     ALLEQRP"
     gene            1965..2783
                     /gene="thiD"
                     /locus_tag="P5558_19695"
     CDS             1965..2783
                     /gene="thiD"
                     /locus_tag="P5558_19695"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRY26601.1"
                     /translation="MTATALTIAGSDSGGGAGIQADLKTFSALGVYGTSAITALTAQN
                     TRAVTGILAVEPAFVAAQIDAVFADIRVDAVKIGMLGGPEVIDAVAERLRHWRPRWVV
                     LDPVMVAKSGDRLLAEAAVAALRDRLLPLASLLTPNLPEAGDLLGEPPVTERRQMPAV
                     AARLQHLGCPNVLLKGGHLGGEESPDVLLHGGEVLWQGGGRVATTNTHGTGCTLSAAI
                     AAFLARGRPLPEAVASAKRYLTGAIRAADGLGIGHGHGPVHHFFELWPKEGTTP"
     gene            2780..3850
                     /locus_tag="P5558_19700"
     CDS             2780..3850
                     /locus_tag="P5558_19700"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY26602.1"
                     /translation="MTTTRRTALFAAAATVAAPAVARSQTPRRWRMVTSWPRDLPGPG
                     VTARRLGERIEAMSGGAIEVELFAAGEIVPAFEVLDAVGEGVAELGHTAAVFWAGKMP
                     VSQLFLAVPFGLTPLEHMAWIEHGGGQALWDELYAGFGVKPFMAGNTGMSMGGWFRRE
                     LHSAADLKGLRFRMPGLGGVLYAPMGIVQVSLPPGETLPALQSGAVDAAEFAGPASDL
                     ALGFYQAAPFYYGPGLHEPNGTGECVVNRALFEGLPADLQAVVTEACRAENAFALAEA
                     ERMNAAALMALVDEHGVQLRTFPDDIVEALRAEAPAVLAPFAAAGGLDARIHDSYVAT
                     LELLRPWSRTSMADFLTARAGG"
     gene            3854..4669
                     /locus_tag="P5558_19705"
     CDS             3854..4669
                     /locus_tag="P5558_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26603.1"
                     /translation="MAVGTLPGERARPRVERRPVAWAVLRPLLTLFGLLAAWQSVVLA
                     TAVPPYILPPPLAVLETLATNWQLVLGHARITGLEILLGFLLGGLLGAGSGLVLAGFA
                     TARRWLLPLFVVSQALPVFALAPLLVLWLGYGMASKIAMAVLIIYFPIAAAFYDGLRR
                     ADPEMLDLVRLLGASRFDTIRLIRLPAALPSLASGLRVAASVAPIGAVVGEWVGSAGG
                     LGYLMLHANARMQIDLMFAALLVLAAMALALYLAVDRLGRRLTPWQAETSSDF"
     gene            4690..5628
                     /locus_tag="P5558_19710"
     CDS             4690..5628
                     /locus_tag="P5558_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26604.1"
                     /translation="MRLAALALALALASPVQAQDKLTVLLDWFVNPDHAPLVIAREQG
                     FFAEHGLEVELIAPADPNDPPKLVAAGQADIAVSYQPQLHLQVAEGLPLRRIGTAVAT
                     PLNSLVVLADGPIEGIADLEGRKVGFSVGGFEDALLGAMLEKHGLTLGDIELVNVNFS
                     LSPALVAGQVDAVIGAFRNFELNQMELIERPGRAFYPEEEGVPAYDELIYVAHVDKLG
                     DPRLQRFMEAVEAATHWIVNHPNEAWEVFAATDPLLDDELNRLAWADTVPRLALSPAA
                     LDHHRYARFAAFLVERGLLEEALPVGGYAVDLFASD"
     gene            complement(5625..6560)
                     /locus_tag="P5558_19715"
     CDS             complement(5625..6560)
                     /locus_tag="P5558_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26605.1"
                     /translation="MLKNKAINAVGIIVTFACIGLFFDSFTNLDFWQLAHLPRMELFA
                     VTATATMIYASLLVAVGIGFASLVRSTGHAMATPQEGIALWGQANIAKYLPGNVMHFA
                     GRQLLARRFDWPQASTTTASVLELALQVLMSCSIGVAGLLMIDRMNTEVASSWLVAAL
                     FLSSAVLTVALLGRLPLAWLPAIARRLLAAFSLEDLRAFSRSTLLYGAFFIGQGLVGW
                     LVYSWLLGVADPAILPGVVAIVLLGWCAGLVVPGAPGGLGVREATTVLLGAPFLDNDS
                     LMLMTILLRVISLAGEGLMFALACQLPRTRRPAAP"
     gene            complement(6722..>7065)
                     /locus_tag="P5558_19720"
     CDS             complement(6722..>7065)
                     /locus_tag="P5558_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY26606.1"
                     /translation="GGTSYSMLQTAAEAYKVLQLQNQSWPALDAFHTMTRGNALALGL
                     AHEIGTLEPGSHADVTVLDLAPTPAIAHRLEAIDRSDLPALLFALMTIGDDRAIRATW
                     VAGKKLHERNG"
BASE COUNT          934 a         2447 c         2586 g         1098 t
ORIGIN      
        1 ctgagccgcc cggtcgcgct cagtcgacgt cgagatcgaa gccgagccgg ccgatcacgt
       61 cgccgagccg ctgccaggaa tcgctgtcat cggcgccggc agccaggccg gcgaggttgg
      121 gcgtcagatc gccgagcgcg tcgtcggact cgtcgaagaa ttcgcagtcg atctcgggca
      181 tggccgggcc gccgggcggg cgtgcccgcg tcgccacttc cgccagccgt tcctgcacct
      241 gggtttcggg ttctcttcgc aagccgccac tcctcgtctc cgggccgcgc tgcctttcgc
      301 gcccggggtc gagcttgcgt ctttccggtt accacggcgt taacggcggt catcctcttg
      361 tcaccggctg ccgggccgcc taactctctc tttgtctcaa cggggtgtcc ctgagttgcg
      421 ggactgagag cgcgaggcag atcgcgaaac ccgtcgaacc tgatccggat gatgccggcg
      481 tagggattga gcggctcctc gaaccgtcca tcaccctccc gccggcaatg cccgggcatc
      541 atgacgaggg gggacggtga ccgatcacga cgacaggtcc aggctggcga gggacgcagc
      601 gcgcgtgctg gcgcggctgc gcgccgagcg gccgcgcgtg cattgcctca ccaatttcgt
      661 cgccatgcag ctcacggcca acctcctgct cgccgccggt gccgtgccgt cgatgaccat
      721 ggacgcgcgc gagatgccgg ccttcgtcga gacgagccgg gcgctgctcg tcaatctcgg
      781 catgctcgac ccctggcgcg aggcggccat tccggtcgcc atcgaggctg ctcatgggct
      841 cggccggccc tgggtgctgg accccgtcaa ggtcgaccga gcccccgccc gccgggcgtt
      901 cgcgaggcgc ctgctcgagc gcgcgccggc cgtgcttcgc tgcaacgcgg ccgaggcgga
      961 gatgctcgag ccggggccag ggatcgtcac cgccgtgacc ggtgccgccg atcgcatcag
     1021 cgggggcggg cgcgccatcg agctcgctgg cggcacgatg ctgatggacc gggtgacggc
     1081 gatgggctgc gccgcctcgg cgctggtcgc cgcctgcctc gcggtcgagc cggacccctt
     1141 cctcgcgacc gtttcgggcc tgctcgtcat gaaggtggcg ggggcgatcg ccgccgagtc
     1201 ggcggccggc cccggcagct tcgtgccgct cttcctcgat gccgtccacg gcctcgatgc
     1261 cgccaccctc cagcgccggg cagaactggc atgaacgtgc cgatcgacat ccgccgggcg
     1321 ctcgccctct atgccgtgct cgacccggcg cgctgccggg gccgcgaccc ggtggagctg
     1381 gtcgccgctg ccgcaagcgg cggggcgacc ctcgtgcagc tccgcgacaa ggcgagcggc
     1441 acgcgcgatc tcgtggccct tgcccaggcg gtcatggcgg cgctcgagcc gcatgccgtg
     1501 ccgctcatcg tcaacgaccg ggtggacgtg gcgctggccg ccggggccgc cggcgtgcat
     1561 ctcgggctca gcgacatgga cccggccgat gcgcggcggc tggtcggccc ggacgtcatt
     1621 ctcggcatca ccgtgcatca cggggccgag gccggggcgg tgccggccgg ccttgccgac
     1681 tatgccgggc tcggcccggt cttcgccacc gccagcaagg acccgggcga cccgcccttg
     1741 gggccgaagc ggctcgcccg gctgatcggc gaggtgcggg ggcaccatca gggcctgccg
     1801 gcctgcggca tcgccggcat cgaccacacg aacgcggctt ccgtcatcgg tgccggtgcc
     1861 gatggcgtcg ccgtgatctc cgacctcttc atggccgacg acgtcgaggc ggcggcacgg
     1921 cggctgcgcc ggatcgtcga tgcggccctt ctggagcagc ggccatgacc gccacggcgc
     1981 tcaccatcgc cggctcggac agcggcggcg gggccggcat ccaggccgac ctcaagacgt
     2041 tctcggcgct gggggtctat ggcacctcgg cgatcacggc gctgacggcc cagaacacca
     2101 gggcggtgac cggcatcctc gcggtcgagc ccgccttcgt cgcggcccag atcgatgccg
     2161 tcttcgccga catccgcgtc gatgcggtga agatcggcat gctcggcggc cccgaggtca
     2221 tcgacgcggt ggccgagcgg ctgcgtcact ggcggccgcg ctgggtcgtg ctcgacccgg
     2281 tgatggtggc gaagagcggc gaccggctgc tcgccgaggc ggcggtggcg gcccttcgcg
     2341 accggctcct gccgctcgcc tcgctgctca cgccgaacct gccagaggcg ggggatcttc
     2401 tgggcgagcc gccggtgacc gagcgccggc agatgccggc cgtggcggcc cggctgcagc
     2461 atctgggttg ccccaacgtg ctgctcaagg gggggcatct cggcggcgag gagagcccgg
     2521 acgttctcct gcatggcggc gaggtgctct ggcagggggg cgggcgcgtg gcgaccacca
     2581 acacgcacgg gacgggctgc acgctctcgg ccgccatcgc cgccttcctc gctcgcgggc
     2641 ggccgctgcc ggaggcggtg gcgagcgcca agcgctacct gacaggcgcc ataagggcgg
     2701 cggacgggct gggcatcggc catggccacg ggccggtgca tcatttcttc gagctgtggc
     2761 cgaaggaagg gaccacccca tgacgacgac ccggcgcacg gccctcttcg ccgctgccgc
     2821 cacggtcgcg gcgccggccg tggccaggag ccagacgccg cggcgctggc gcatggtgac
     2881 ctcctggccg cgggacctgc ccggacccgg tgtcacggcg cggcggctgg gcgagcgcat
     2941 cgaggcgatg agcggcgggg ccatcgaggt ggagctcttc gccgccggcg agatcgtgcc
     3001 ggccttcgag gtgctggacg cggtgggcga gggggtggcg gagctcggcc acacggcggc
     3061 tgtcttctgg gccggcaaga tgccggtctc gcagctcttc ctcgccgtgc ccttcggcct
     3121 gacacccctc gagcacatgg cctggatcga gcatggcggc ggccaggcgc tctgggacga
     3181 gctctatgcg gggttcggcg tcaagccctt catggcgggc aacaccggca tgtcgatggg
     3241 cggctggttc cgccgtgagc tgcattcggc ggcggacctg aagggtctgc gctttcgcat
     3301 gccggggctg ggtggcgtgc tctatgcgcc catgggcatc gtccaggtgt cgctgccgcc
     3361 gggcgagacc ctgccggccc tgcagagcgg tgccgtggac gcggccgagt tcgccggccc
     3421 cgccagcgac ctggcgctcg gattctatca ggccgccccc ttctattacg gcccgggcct
     3481 gcacgagccg aacggcacgg gcgagtgcgt ggtcaaccgg gcgctcttcg agggcctgcc
     3541 tgcggacctg caggcggtgg tcaccgaggc ctgccgggcg gagaacgcct ttgcgctggc
     3601 cgaggccgag cggatgaacg cggcagcgct catggcgctg gtcgacgagc atggcgtgca
     3661 gttgcggacc tttcccgacg acatcgtcga ggccttgcgc gccgaggcgc ccgcggtgct
     3721 cgcccccttc gccgcggcgg gcgggctgga cgcgcgcatc cacgattcct acgtggcgac
     3781 gctggagctg ctccgcccgt ggtcccggac ctcgatggcg gatttcctca cggcccgtgc
     3841 cggcggctga gcgatggcgg tcggcacgct tccgggggag agggcgaggc cgcgggtcga
     3901 gcggcgcccg gtcgcctggg ctgtcctgcg gccgctgctg acgctcttcg gcctcttggc
     3961 ggcctggcaa agtgtggtgc tggccactgc cgtgccaccc tacatcctgc cgccgcctct
     4021 ggcggtgctc gagacgctgg cgaccaactg gcagctcgtt ctcggccatg cgaggatcac
     4081 cgggctcgag attcttctgg gctttctcct gggtggcctg ctgggggcgg ggtccggcct
     4141 cgtgctcgcc ggcttcgcca cggcgcggcg ctggctgctg ccgctcttcg tcgtcagcca
     4201 ggccttgccg gtctttgccc tcgccccgct gctggtgctc tggctcggct acggcatggc
     4261 gtcgaagatc gccatggcgg tgctcatcat ctatttcccg atcgccgccg ccttctatga
     4321 cgggctccgg cgggccgatc ccgaaatgct ggatctcgtg cgcctgctcg gtgcctcgcg
     4381 cttcgacacc atccgcctga tccgcctgcc ggcggcgctg ccgtcgctcg cctcggggct
     4441 ccgggtggcg gcctcggtgg cccccatcgg ggcggtggtc ggcgagtggg tggggtcggc
     4501 cggcgggctc ggctacctca tgctgcacgc caatgcgcgc atgcagatcg acctgatgtt
     4561 cgccgcactc ctcgtcctcg cggccatggc gctcgccctc tacctcgccg tcgaccggct
     4621 gggccgtcgc ctgacgccct ggcaggccga gacctcgtcc gacttctgat ctggagaatt
     4681 ttcgtgatcc tgcgtctcgc cgccctggcc ctcgccctcg ccctcgcctc gccagtccag
     4741 gcccaggaca agctgacggt gctgctcgac tggttcgtca acccggacca cgcgccgctc
     4801 gtcatcgcga gggagcaggg cttcttcgcg gagcacgggc tcgaggtgga gctgatcgcc
     4861 ccggccgacc ccaacgaccc gccgaagctg gtggccgccg gtcaggccga tatcgcggtg
     4921 agctaccagc cgcagctcca tctgcaggtg gccgagggcc tgccgctccg gcgcatcggc
     4981 acggcggtgg cgacgccctt gaacagcctc gtggtgctgg ccgacgggcc gatcgagggc
     5041 atcgccgacc tcgaggggcg caaggtcggc ttctcggtcg gcggcttcga ggacgcgctg
     5101 ctcggcgcca tgctggagaa gcacgggctg acgctcggcg atatcgagct ggtcaacgtc
     5161 aacttctcgc tctcgccggc gctcgtcgcc ggccaggtcg atgcggtgat cggtgcgttt
     5221 cgcaatttcg agctcaacca gatggagctg atcgagcggc cgggccgcgc cttctacccc
     5281 gaggaggaag gggtgccggc ctatgacgag ctgatctacg tggcgcatgt ggacaagctc
     5341 ggcgacccgc ggctgcagcg cttcatggag gccgtcgagg cggccaccca ctggatcgtc
     5401 aaccatccaa acgaggcctg ggaggtcttc gccgccacgg accccctgct cgacgatgag
     5461 ctgaaccggc tggcctgggc ggacaccgtg ccgcgcctgg cgctcagccc ggcagccctc
     5521 gatcaccacc gctacgcccg cttcgccgcg ttcctggtgg agcgggggct gctcgaggag
     5581 gcgctgccgg tcggcggcta cgcggtcgat ctcttcgcca gcgactaggg agccgccggg
     5641 cggcgggtgc gggggagctg gcaggcgagg gcgaacatca ggccttcgcc ggcgaggctg
     5701 atgacccgga gcaggatggt catcagcatg aggctgtcat tgtcgaggaa gggggcgccg
     5761 agcagcaccg tcgtcgcctc gcgcacaccg agcccgccgg gggcacccgg cacgacgagg
     5821 ccggcgcacc agccgagcag cacgatggcg acgacccccg gcaggatcgc cggatcggcg
     5881 acaccgagca gccaggaata gaccagccag ccgaccagcc cctggccgat gaagaaggcc
     5941 ccgtagagca gggtcgagcg cgagaaggcg cgcaggtcct cgaggctgaa ggcggcgagc
     6001 aggcggcggg cgatggccgg cagccacgcc aggggcaggc ggccgagcag ggcgacggtg
     6061 agcacggccg aggagaggaa cagggcggcg acgagccagg aggaggcgac ctcggtgttc
     6121 atccggtcga tcatcaggag cccggccacg ccgatcgagc aggacatcag cacctgcagc
     6181 gccagctcca gcaccgaggc agtggtcgtg ctcgcctgcg gccagtcgaa ccgccgcgcc
     6241 agcaattgcc ggccggcgaa gtgcatgacg ttgcccggca ggtacttggc gatgttcgcc
     6301 tggccccaga gcgcgatgcc ctcctggggg gtggccatgg cgtgaccggt ggaccgcacc
     6361 aacgaggcga agccgatgcc gacggccacc agcaggctgg cgtagatcat tgtcgccgtc
     6421 gccgtgaccg caaacagctc cattcgcgga agatgagcga gctgccagaa atcgagattg
     6481 gtgaagctgt cgaagaacaa cccgatgcag gcgaatgtca cgatgatgcc gacggcattg
     6541 attgccttgt ttttgagcat agggcctttc gtctcgacag atcggagggg gaaaccgcgt
     6601 gtcgcatcgg accctgggct ctaggatatc tgatccctgt agcgcggcga tcaagatggc
     6661 gctatgataa ttttgtcgac gaatcggcga tattttgcgg aataggcatg tcgggcaggg
     6721 ttcagccgtt tcgctcgtgc agcttcttgc ccgccaccca ggtggcgcga atggcccggt
     6781 cgtcgccgat ggtcatgagc gcgaagagca gggccgggag gtcgctgcgg tcgatcgcct
     6841 cgaggcgatg ggcgatggct ggcgtcggcg ccaggtcgag cacggtgaca tcggcatggc
     6901 tgccgggctc gagcgtgccg atctcgtggg cgaggccgag ggccagggca ttgccgcgtg
     6961 tcatggtgtg gaacgcgtcg agcgccggcc agctctggtt ttggagctgg aggaccttgt
     7021 aggcctcggc ggcggtctgc agcatcgagt aggaggtgcc gccgc
//
LOCUS       DAOTFI010000241         7034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12755_length_7034_cov_7.046282, whole genome shotgun sequence.
ACCESSION   DAOTFI010000241 DAOTFI010000000
VERSION     DAOTFI010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12755_length_7034_cov_7.046282"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="P5558_19725"
     CDS             complement(<1..252)
                     /locus_tag="P5558_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRY26607.1"
                     /translation="MIIPSIDLMGGQAVQLVGGEAMAIEAGDPLPIAERFAVAGPLAV
                     IDLDAALKQGSNRDLIERLVARFDCHVGGGIRDVETALFW"
     gene            complement(249..1001)
                     /gene="hisF"
                     /locus_tag="P5558_19730"
     CDS             complement(249..1001)
                     /gene="hisF"
                     /locus_tag="P5558_19730"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRY26608.1"
                     /translation="MLTKRIIPCLDVSHGRVVKGVRFQGLRDAGDPVELALAYQRQGA
                     DEIVILDISATPEGRGHQHATVERVREGLAIPLTIGGGVRTAADAQALLEAGADKVSI
                     NSAAVADPSLIDAIAGRFGSQCCVVAIDAALREGRFEVLVRGGRDGTGKDAVAWAREA
                     QDRGAGEILLTSWDRDGTREGYDLALTRAVAEAVHLPVIASGGADHAGHVAEAFAAGA
                     DAVLAASIFHDGDLTVGDVKLHLMEAGVVVRP"
     gene            complement(1001..1597)
                     /gene="hisH"
                     /locus_tag="P5558_19735"
     CDS             complement(1001..1597)
                     /gene="hisH"
                     /locus_tag="P5558_19735"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRY26609.1"
                     /translation="MSREVAIVPTGTANLASVAAAFRRLGAVPVVVEDAAQLARASHV
                     MLPGVGAFGASLERLDALGLASALRERIAADLPTIAICVGHQLLFEASEESPGAAGLG
                     VVAGTVTRFPAGIRVPQFGWNEIQAGEGCRLLESGYAYFANSYRATAAPGWHVALAEH
                     GGPFVAGMERGRVIGCQFHPELSGDYGAALLSRFLELG"
     gene            complement(1594..2184)
                     /gene="hisB"
                     /locus_tag="P5558_19740"
     CDS             complement(1594..2184)
                     /gene="hisB"
                     /locus_tag="P5558_19740"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRY26610.1"
                     /translation="MPTERRAVIERHTSETEIRLSLTLDGSGATSIDTGLGFFDHMLT
                     ALIKHSRFDLELACSGDLHVDDHHTVEDCALALGQALDQALGERRGIRRFAHAYAPLD
                     EALARVVVDLSGRPAPHVELGLVRPRIGDLATENLVHFFQSLAIAGRMALHVDVLKGT
                     NDHHRAEAAFKALALALRDAVILTEHKDVPSTKGLL"
     gene            complement(2205..3086)
                     /gene="hisG"
                     /locus_tag="P5558_19745"
     CDS             complement(2205..3086)
                     /gene="hisG"
                     /locus_tag="P5558_19745"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRY26611.1"
                     /translation="MSFPSTGPAALVHLALPKGRMQEAVVRLLGDAGVRLTYDRRGYR
                     PSLSLPEFEAKILKPQNIVEMLDVGSRDLGFAGADWVAEKSVDLVELVDTGLDPVRIV
                     AAADHRFLVDGRPPKERFIVATEYEKLTTAWLVTHGHDARIVRSYGATEVFPPEDADC
                     IVDNTATGATLKANGLTIFDELLCSSTRLYVHPRSLDDPAKRPVIEHFALLIRSVLDA
                     RQRVMLEVNVSTEALEPVVDVLPCMREPTISPLHGEGGFAVKAAVPRADLPSLIRRIK
                     ERGGTDIVVSEIAQIVP"
     gene            complement(3191..5317)
                     /locus_tag="P5558_19750"
     CDS             complement(3191..5317)
                     /locus_tag="P5558_19750"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRY26612.1"
                     /translation="MMMLNQHGGGSKPFAALPRRIDTGEPAPADRRSTGRALALGAPL
                     SASFMGLLLLRGDAAAAGESGDEPAAGSSTGRDVTSAGSGGDAGAGALSLAAMGLGIS
                     SAAAATLVGDGGVIDAAALTALGEASAVDAEALDRAAEAQPAEHGAAPESVPPVPPEI
                     GDVTIAFGSEAPFELPTYTSDEPGLPEENLGDLGDPRLIDRSPIHADAGNGTDGQSDG
                     GGDGGSSTGQAGTPDEAVVGLRITGTSGGDYLVGSDGNDVIDAVGGRNTVLAGAGDDH
                     VIGGLGNDTLHGGPGNDTIEGRAGQNIIYGDEGDDRLFGGTGNDKLYGGSGNDRLDGV
                     AGANRLEGGPGDDTLVINGPRDIAIETSGGGNDTLEVASGFGAALRGRFTTLAPDGTA
                     TFVIGDEPGRTFPAEVNGYLYQLDPEVENVRLMGSDNHDVLGDSNANVITGNDGDNLL
                     YGGSGDDILYAGGGNDVLRGDTGNDTLYAGAGHDVLFGEAGDDVLYGGAGESELHGGE
                     GDDLYVFGLAEGGRATVFDHEGSNRLRFDGPSDPTALGARLEGDDLVLDDAGADVVRI
                     ADYAGHRDAFAGLEVGGETVSLDRFLEATAARAAAVAAGDDLLAIYLGPQSVEADGDI
                     LDPARLGEDAPAHDAPFALYEDTGASGFEAAATTTDAGGAGADDMIGSFMRAEPLWIG
                     PEEGVFVPDADDLRGATGDEAQHARA"
     gene            5546..5869
                     /locus_tag="P5558_19755"
     CDS             5546..5869
                     /locus_tag="P5558_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26613.1"
                     /translation="MSWTCKLYRHFETMATGGQALSGLALITAFGACAPAAEGPPVPA
                     PAPDNAVQVSPELYMVPAGEDDVGCPMFQPWSPTLMVVQALHWRSADGSFTLDRNAAD
                     CPPPT"
     gene            complement(6009..>7034)
                     /locus_tag="P5558_19760"
     CDS             complement(6009..>7034)
                     /locus_tag="P5558_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26614.1"
                     /translation="GRGYTVEMGGDSGSGGREGGQALAGFVDRQRRGEVTRTEAGGLS
                     VDFARLATGSEAAPVVAGSDLGSVDARWELGDRLRVRSFAGSAAAFDETEREVLPEGH
                     GFGGLANEAGLALDSSFGDAWAASIDLQAGKVESGELGAGARLAELGLERRLSEALSL
                     RLGFGVLDEADGPLGSSGSGALALDGAVTHYVNLTGRLALTERLGLFGRAELGTTGGV
                     DGSGMLTRTGTMRSSAFALGLTAADLLQTSDRLGFSILQPLRVDSGTAELDVPVAQDA
                     TGAVLFARQEADLAPDGREIDVELTYGWDVGRARLQAAAMLRLEPDHDASAEPELLLG
                     FAWHLPF"
BASE COUNT         1089 a         2536 c         2381 g         1028 t
ORIGIN      
        1 ccagaagagc gccgtttcga cgtcacggat gccgccgccg acatggcagt cgaagcgggc
       61 caccagccgc tcgatcaagt ccctgttcga gccctgcttc aaggctgcat ccaggtcgat
      121 gacggccaag ggccccgcca cggcgaagcg ctcggcgatc ggcaagggat cgcccgcctc
      181 gatcgccatg gcctcgccgc cgacgagctg gacagcctgg ccgcccatga ggtcgatgga
      241 ggggatgatc acggccgcac caccacgcca gcttccatca gatgcaactt gacgtcgccc
      301 acggtcaggt ccccgtcgtg gaagatggag gccgccagca ccgcatccgc gccagcggcg
      361 aaggcctcgg ccacatggcc ggcatgatcg gctccgcccg aggcgatcac cggcagatgc
      421 accgcctcgg ccaccgcccg ggtcagcgcc aggtcgtagc cctcccgggt gccgtcccgg
      481 tcccatgagg tgagcaggat ctcgccggcc ccacggtcct gcgcttccct ggcccaggcc
      541 accgcatcct tgcccgtgcc gtcgcggccg cccctgacca gcacctcgaa tcgtccctcg
      601 cgcagggcgg catcgatggc gacgacgcag cactggctgc cgaaacggcc ggcgatggcg
      661 tcgatcagcg aggggtcggc cacggccgca ctgttgatcg agaccttgtc ggcaccggcc
      721 tcgagcaggg cctgcgcatc cgccgccgtc ctgaccccgc caccgatggt gagcggaatg
      781 gcgaggccct cgcgcacccg ctcgaccgtg gcatgctggt gaccgcggcc ctccggcgtc
      841 gccgagatgt cgaggatgac gatctcgtcg gctccctgcc tctggtaggc gagtgccagc
      901 tcgaccgggt caccggcatc ccggagcccc tggaagcgca cgcccttgac cacccggccg
      961 tggctgacgt cgaggcaggg gatgatccgc ttcgtgagca tcagccgagc tccagaaagc
     1021 gcgacagcag ggctgcacca tagtcacccg agagctccgg atggaactgg cagccgatca
     1081 cccggccccg ctccatgcca gcaacgaagg ggccaccatg ctcagccaac gcgacatgcc
     1141 agccgggtgc cgccgtggcc cggtaggaat tggcaaaata agcgtagccg ctctcgagca
     1201 gccggcaacc ctcgcccgcc tggatctcgt tccagccgaa ctgcggcacc cggatcccgg
     1261 ccggaaaacg cgtcaccgtg ccggcaacga cacccaggcc cgcggcgccc gggctctcct
     1321 cgctcgcctc gaagagcaac tggtggccaa cgcagatggc gatggtcggg agatcagcgg
     1381 cgatccgctc ccggagcgcg ctggccagcc ccagcgcatc gagccgttcc agcgacgcgc
     1441 cgaaggcccc cacgcccggc agcatgacat ggctggcgcg agccagctgc gccgcatcct
     1501 ccaccaccac cggcacggcc ccgagccgcc tgaaggcggc ggcgaccgac gccagattgg
     1561 ccgtcccagt cgggacgatg gcgacctcgc gactcaaagc agccccttgg tgctgggcac
     1621 gtccttgtgc tcggtcagga tcacggcgtc gcgcagggca agcgccaggg ccttgaaggc
     1681 ggcctcggcc cggtggtggt cgttggtccc cttcagcacg tcgacatgca gcgccatgcg
     1741 tccggcgatc gccagcgact ggaagaaatg cacgaggttc tcggtcgcca agtcgccgat
     1801 ccgggggcgg acgaggccga gctcgacatg cggcgccggc cggccggaga ggtcgaccac
     1861 gacccgcgcc agggcttcgt cgagcggggc ataggcatgg gcgaagcggc ggatgccgcg
     1921 ccgctcgccc agcgcctggt caagcgcctg cccgagtgcc agggcgcagt cctcgaccgt
     1981 gtggtggtcg tcgacatgca ggtcgcccga gcaggccagc tcgagatcga agcggctgtg
     2041 cttgatcagg gccgtcagca tgtggtcgaa gaaaccgagc ccggtatcga tgctggtggc
     2101 gccgctgccg tcgagcgtga ggctgaggcg gatctcggtc tcggaggtgt gccgctcgat
     2161 cacggcacgg cgttcggtcg gcatgtccgt cacggcagga tcgctcatgg cacgatctgg
     2221 gcgatctcgg agaccacgat atcggtcccg ccacgctcct tgatgcgccg gatcagcgac
     2281 ggcaggtcgg cgcgcggcac ggctgccttg accgcgaagc cgccctcgcc gtgcaggggc
     2341 gagatggtcg gctcgcgcat gcaagggagc acgtcgacca ccggctcgag cgcctcggtc
     2401 gagacattga cctcgagcat cacgcgctgg cgcgcgtcca gcaccgagcg gatgagcagg
     2461 gcgaaatgct cgatcaccgg ccgcttcgcc ggatcgtcga gcgagcgcgg gtggacatag
     2521 agccgggtcg aggagcacag gagctcgtcg aagatggtga ggccgttggc cttcagcgtc
     2581 gcccccgtcg ccgtgttgtc gacgatgcag tcggcatcct cgggcgggaa cacctcggtg
     2641 gcgccgtagg agcggacgat ccgggcgtcg tggccgtgcg tcaccagcca ggccgtggtc
     2701 agcttttcgt attcggtggc gacgatgaag cgctctttcg gcggcctgcc atcgaccagg
     2761 aagcggtgat cggcggcggc gacgatcctg accggatcga ggcccgtatc gaccagctcg
     2821 accaggtcga ccgacttctc ggcgacccag tcggccccgg cgaagccgag gtcgcggctg
     2881 ccgacatcga gcatctcgac gatgttctgc ggcttgagga tcttcgcctc gaactcgggc
     2941 agcgaaagcg acggccgata gccgcggcgg tcgtaggtga ggcgaacgcc agcgtcaccc
     3001 aggagccgga cgacggcctc ctgcatgcgg cccttgggca gggccaggtg gacgagcgcg
     3061 gcggggccgg tggacggaaa gctcacggga tggcgggcct tgtcggacgg gtttggacag
     3121 cagatcgggc gccgtgcccc gagggacgca gcgcccgacg ctatagagaa gccgcccgcg
     3181 gccgtaaagc tcaggccctg gcgtgctgcg cctcgtcgcc ggtggcaccc cgcaggtcgt
     3241 cggcgtccgg cacgaagacg ccctcctcgg ggccgatcca gagcggctcg gcgcgcatga
     3301 agctgccgat catgtcgtcc gcgccggccc cgcccgcatc ggtcgtcgtg gcggcggctt
     3361 cgaagccgct ggcgcctgtg tcctcgtaga gcgcgaaggg tgcatcgtgc gctggcgcgt
     3421 cctcgccgag ccgcgccgga tctaggatgt cgccatcggc ctccaccgat tgcgggccga
     3481 gatagatggc gagcaggtcg tcgccggccg caactgctgc cgcccgggcc gccgtcgcct
     3541 cgaggaaccg atcgagcgag acggtctcgc caccgacctc gagaccggca aaggcgtcgc
     3601 gatggccggc gtagtcggcg atccgcacga catcggcgcc ggcatcgtcg aggaccaggt
     3661 catcaccctc gaggcgcgcg cccagtgctg taggatcgct aggcccgtcg aaccgcagcc
     3721 ggttgctgcc ctcgtggtcg aagacggtgg cccggccacc ttccgcgagc ccgaagacat
     3781 agagatcgtc accctcgccg ccgtgcagct cgctctcgcc ggcgccgcca tacagcacgt
     3841 cgtcgccggc ctcgccgaag agcacgtcat ggcccgcccc ggcgtaaagc gtatcattac
     3901 cggtatcgcc gcggagcacg tcgttgccac cgccggcata gaggatgtcg tcgccgctcc
     3961 cgccatagag caggttgtcg ccgtcattgc cggtgatgac gttggcgttg ctgtcgccca
     4021 ggacatcgtg attgtcgctg cccatgagcc ggacgttctc gacctcgggg tcgagctggt
     4081 aaagatagcc gttgacctcg gccggaaacg tccggcccgg ctcgtcgccg atgacgaagg
     4141 tcgcggtccc gtccggggcg agggtcgtga agcgaccgcg gagcgctgcg ccgaagccgg
     4201 aagccacttc gagcgtgtcg ttgccgccgc cgctcgtctc gatcgctatg tcgcgtgggc
     4261 cgttgatcac cagggtgtcg tcccccggcc caccctcgag ccggttggcg ccggccaccc
     4321 cgtcgagccg gtcattgccg ctgccgccat agagcttgtc gttgccggtg ccaccgaaaa
     4381 gcctgtcgtc accctcgtca ccgtagatga tattctgtcc agctctaccc tcgattgtat
     4441 cgttgcccgg cccgccatgc aaggtgtcgt tgccgaggcc gccgatcaca tgatcgtcgc
     4501 cggcaccggc gagaaccgtg ttccgcccgc ccacggcatc gatcacgtcg ttgccgtcgc
     4561 tgccgacgag gtagtcgccg ccgctggtgc cggtgatccg caggccgacc acggcttcgt
     4621 caggtgttcc ggcctgtccc gtcgacgagc cgccatcccc gccaccgtca ctttgcccgt
     4681 cggtgccatt cccggcatcg gcatggatcg gcgagcgatc gatcaggcgc gggtcgccga
     4741 gatcgcccag gttctcctcc ggcaggcccg gctcgtccga ggtgtaggtt ggcagctcga
     4801 agggggcctc gctgccgaag gcgatggtga cgtcgccgat ctcgggtggc accggcggca
     4861 ccgattcggg cgccgcgcca tgctcggcag gttgggcctc ggcggcccgg tcgagcgcct
     4921 ccgcgtcgac cgccgatgcc tcgcccaggg cggtgagcgc cgccgcgtcg atgacgccgc
     4981 cgtcgccgac cagcgtcgcc gccgcggccg acgagatgcc gagccccatg gccgccagcg
     5041 aaagcgcccc ggcgcccgca tcgccaccgc tgcccgcaga cgtgacgtcc cggccggtgg
     5101 acgatccggc agccggctcg tcgccgctct ctcccgccgc cgcggcgtcg ccacgcagca
     5161 acaggagccc catgaagctc gccgagaggg gtgcaccgag cgccagtgca cgaccagtcg
     5221 agcgccggtc cgcaggcgcc ggctcgccgg tatcgattcg ccgcggcagc gctgcgaacg
     5281 gcttgctgcc gccaccatgc tggttcaaca tcatcatacg gtgctcccac gccgattgta
     5341 gctcgggcgt tggtgtcact cgatgacgag ccgatgggac ccggcggttg aagaagtcag
     5401 acggtgcttt cccttgagac gaaccatagc cagctaaccg cctattttca aaggtttttt
     5461 tggaaatctc tcatcttacg gtagaacgtt aattgataga tgcttaacca cagattttag
     5521 tttccaacct gaggaacgga ccgcaatgag ctggacttgc aagctttatc ggcatttcga
     5581 gacgatggcc acgggcggtc aggccctgtc cgggctcgcc ctgatcacgg ccttcggtgc
     5641 ctgcgcgccc gcggctgagg gcccgcccgt gccggcccca gcacctgaca atgccgtgca
     5701 ggtaagccct gagctctaca tggtgccggc cggcgaagat gacgtcggct gcccgatgtt
     5761 ccagccctgg tcaccgacgc tgatggtggt ccaggcgctg cactggcgaa gcgccgacgg
     5821 cagcttcacc ctcgaccgga acgccgccga ctgcccgccg ccgacgtagt ggccacccgc
     5881 aaagcgtgtt cccgcggtga ccgtggacga ggaaggcaag gcggtcggcg cgcagcgtag
     5941 tcaatctacg tgagcaccga tcgtccgcgg cctgacgaag tccacggtca ccgtgggaac
     6001 acgctcgttc agaagggcag gtgccaggcg aagcccagca gcagctcggg ctcggccgac
     6061 gcatcgtggt ccggctcgag gcgcagcatc gcggccgcct gcagacgcgc ccggccgacg
     6121 tcccagccat aggtcagctc gacatcgatc tcgcggccat ccggcgccag atcggcctcc
     6181 tgccgcgcga agagcaccgc ccccgtcgca tcctgggcaa ccggcacgtc gagctcggcc
     6241 gtgcccgaat cgacccgaag cggctgcagg atcgagaagc ccagccgatc cgacgtctgc
     6301 agcaggtcgg cggcggtcag gcccagggcg aaggcgctgc tgcgcatggt gccggtccgg
     6361 gttagcatgc cgctgccatc cacaccgccc gtcgtcccga gctccgcccg accgaagaga
     6421 ccgaggcgct cggtcagcgc cagccggccg gtcagattga cgtagtgcgt cacggcaccg
     6481 tccaaagcga gcgccccgct gccgctgctg ccgagcggcc catcggcctc gtcgagcacg
     6541 ccgaagccca ggcggaggct gagcgcctcg ctgagcctgc gctcgaggcc gagctcggca
     6601 aggcgtgcgc cggcgccgag ctcgccgctc tcgaccttgc ccgcctgcag atcgatcgag
     6661 gccgcccagg cgtcgccgaa ggagctgtcc agcgccaggc cggcttcgtt ggcgaggccg
     6721 ccgaagccgt gtccctcggg cagcacctcg cgctccgttt cgtcgaacgc agcggctgac
     6781 ccggcgaagc tgcggaccct gagccggtcg ccgagctccc agcgcgcgtc gacgctgccg
     6841 agatcgctgc cggcgaccac cggcgccgcc tcgctgccgg tggccagcct ggcgaaatcg
     6901 acggacaggc cacccgcctc cgtccgtgtc acctcgccgc gccgctggcg atcgacgaag
     6961 cccgcgagcg cctgcccgcc ttcacggccc ccgctgccac tgtcgccgcc catctcgacg
     7021 gtgtaacccc gccc
//
LOCUS       DAOTFI010000242         6998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12829_length_6998_cov_7.637909, whole genome shotgun sequence.
ACCESSION   DAOTFI010000242 DAOTFI010000000
VERSION     DAOTFI010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12829_length_6998_cov_7.637909"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..815)
                     /gene="gph"
                     /locus_tag="P5558_19765"
     CDS             complement(141..815)
                     /gene="gph"
                     /locus_tag="P5558_19765"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199761.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HRY26615.1"
                     /translation="MSLSAVVFDLDGTLVDTLPDLHAAVRALLGESGLDTPAAAEVRT
                     MIGDGARRLVERALAWAKAPVDEAGLDTAYRRFLEIYGAAPCEASGLYAHVETTLGAL
                     AAQGLQLGVCTNKPQAPSEAILETLGVSRFFAAVVGGDAVPRRKPDPAHLLDVLDRLG
                     AKPAAAVMVGDSHNDVAAARAAGVRCILVDYGYTAVPARDLGADRVIGGFAELEAALA
                     GLPSST"
     gene            918..2297
                     /gene="glmU"
                     /locus_tag="P5558_19770"
     CDS             918..2297
                     /gene="glmU"
                     /locus_tag="P5558_19770"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF010933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRY26616.1"
                     /translation="MAAADPIDAEKLSIVILAAGRGTRMCNDRPKVLHELAGKSLIAH
                     VLGLASSLRPTLLVTVLGAGMEDVAAEVRKLAPDGRIVIQDPPHGTGHALAVAAPALP
                     ESGAVLVLYGDTPLLTRATLEKLLERRARDGAAVTVLGMRPPDPAGYGRLRADPDGRL
                     HAIVEHKHCDAALLASDLCNAGVLALAADRLRHLLDELPRQPENGEYYLTDTVGLAVA
                     KGWSCSAVEGSWIEGQGVNTQAQLAKVAGFFQARRRAELMEQGVIMEAPDTVMLSADT
                     AIGAGAVIEPFVVFRPGVTVEPGAVIHAHSYLEQARVGAGAEIGPFARLRPGAEIGDR
                     AKIGNFVEVKNTRLGAGAKASHLTYLGDCEVGAGANIGAGTVTCNYDGFMKHRTVIGE
                     EAFVGSNTALVAPVVIGERVVVGAGSVLTAYVPADALALSRVPQRVLEGAGARLRTRL
                     AHEKMRRKS"
     gene            2369..3481
                     /locus_tag="P5558_19775"
     CDS             2369..3481
                     /locus_tag="P5558_19775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY26617.1"
                     /translation="MRRLTAICAAWVVVAALAWAVVPTFGGGALAQGLADVRTGNEAF
                     RQGDYAAAVTAYTDAIRSGELVGEALAITYNNRGVAYGEAGDFESAIADYSQALALRP
                     GDATTIRNLRVAHVRRGEVLFASGSYDAAIADFDAAIEMQADHYLAYLRRGELYAERG
                     ELDLARADLEQAAALNPGDPEIIAALERAESREPATVAADEPGKASPPETATASAPAA
                     SEAPVAAAEPVEAAEPEPAAAPATTAEAAPPATETADSPVVAAVPERPATPEPAPAEP
                     AAAAADAPATTAAAVPTPAPAPAESRAAGNGETWRVVSAVNYRAGPDNDAERLGTVDA
                     GRMVTVTGDTLGWKHVTLPNGDQGYIYKKWLEPVTP"
     gene            3646..5043
                     /locus_tag="P5558_19780"
     CDS             3646..5043
                     /locus_tag="P5558_19780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRY26618.1"
                     /translation="MLCRLALAVFLLALGAATASPVQANSKYAAVVLDYATGEVLHSR
                     RADVALYPASLTKMMTLYMLFEALERGELRLGSKLDVSRRAAGMPASKLGLGAGSTIT
                     VEQAIEAIVVKSANDVAVVIAEALAGSEPRFADAMTRRARAIGMRHTTFKNASGLPDR
                     QQKSSAADMARLAYRLISDHPRYYPYFSRRSFSFNGVNHRGHNRLLVSYAGMDGLKTG
                     YTRASGFNLAATARRSGERVIVVVFGGRSARTRDKEVARLLDLGFERVRERRPAPAIA
                     LLPQAKPGTTMAAVAVVDTADDAIAPEAGPVPALRPGTLVEVPGVRPAPVVAVAPAVS
                     VLDALPPAERAVIVAAAANAPLPAPNPQRLVVSGGAGTAATGPFAVQVGAFHDSNLAR
                     RAAQTAARLVPQILMQGAIDVSPLKGRRRTVYRARVAGFERAVADEACQMLQQHHQEC
                     FVVQTRGYEVAGRTS"
     gene            5204..6541
                     /locus_tag="P5558_19785"
     CDS             5204..6541
                     /locus_tag="P5558_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26619.1"
                     /translation="MGEGADIRGGRLPAAAYDENFADLQPPLARQEAVVEAARCYFCY
                     DAPCVQACPTGIDIPSFIRKIQTGNLKGSALDILEANIMGGTCARVCPTEVLCEEACV
                     RTVQEGKPVRIGRLQRYATDALFATGIQPFSRAASTGRRIAVVGAGPAGLACAHALAR
                     RGHDVVVFERRAKAGGLNEHGIAAYKMANDFAAREVAFILGIGGIELHLGQALGRELT
                     LDALRRDHDAVFLGVGLGTTNRLGLAGEELEGVLDAVDFIEELRQSPDKATLPVGRHI
                     VVIGGGNTAIDIAVQTKRLGAETVTLVYRRGAGEMGATGHEQEIAQLNGVLIRHWARP
                     VTLEAAGGRVAGVTFEHTRLDAGGRLEGTGERFTLAADQVFKAIGQKLLADELAGSGL
                     AIEAGKLRVDDELRTSLDGVWAGGDAVAGQDLTVAAVEDGKRAARSIDRALGN"
     gene            6559..>6998
                     /locus_tag="P5558_19790"
     CDS             6559..>6998
                     /locus_tag="P5558_19790"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554193.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HRY26620.1"
                     /translation="MADLRCTIAGVKSPNPFWLASAPPTDKAYNVVRAFRAGWGGVVW
                     KTLGEDPPIVNVSSRYGAVHFRGHRMAGFNNIELITDRTLEVNLREITQVKRDWPDRA
                     MVVSLMVPCEEASWQAILARVEATGADGVELNFGCPHGMSERGMG"
BASE COUNT         1029 a         2475 c         2479 g         1015 t
ORIGIN      
        1 gaacctgtga cccctcgcgt gtgaagcgag tgctctcccg ctgagctacg cgcccttcgg
       61 cccccgacga tcgaatgccg cgctctaagt cgtggccgct tccctgtcaa gctgctgtca
      121 agccgctggc cgggcgtggc tcaggtgctg gacggcaggc cggcgagcgc cgcctcgagc
      181 tcggcgaaac ccccgatcac ccgatcggcg ccgaggtcgc gggccggcac cgccgtgtag
      241 ccgtagtcga cgaggatgca gcgcacgccg gcggccctgg ccgcggccac gtcgttgtgg
      301 ctgtcgccca ccatgaccgc cgctgccggc ttagcaccga gacggtcgag cacatcgagg
      361 agatgggcgg gatcgggctt gcgcctcggc accgcgtcgc cgccgaccac ggccgcgaaa
      421 aacctgctga cgccaagggt ttcgagaatc gcctcgctcg gcgcctgggg cttgttggtg
      481 cagacgccga gctggaggcc ctgcgcggcg agcgccccga gtgtcgtctc cacatgcgcg
      541 tagagcccgc tcgcctcgca cggtgccgcc ccgtaaatct cgaggaagcg ccgatacgcc
      601 gtatcgagcc cggcctcgtc gacaggtgcc ttcgcccagg cgagggcgcg ctcgaccagg
      661 cgtctggcac catcgccgat catcgtccgc acctcggcgg cggcgggggt gtcgagcccg
      721 ctctcgccga gcagcgcccg cacggcggcg tgcagatcgg gaagcgtgtc gacgagggtg
      781 ccgtcgagat cgaagaccac ggccgagagg gacatgagga gtcacctgga gcagatgttg
      841 tgagccgcgc cgaacttggc tagttcagag ccgctcgtcg agcccgcccg gctgcacttt
      901 tgccctggag ttgccgaatg gccgccgctg atccgatcga tgccgagaag ctcagcatcg
      961 tcatcctcgc cgccgggcgc ggcacgcgca tgtgcaacga ccggcccaag gtgctgcacg
     1021 agctcgccgg gaagagcctg atcgcccacg tgctgggcct cgcctcgtcg ctgcggccga
     1081 cgctgctggt taccgtgctc ggcgccggca tggaggacgt cgcggccgag gtccgaaagc
     1141 tggcgccgga cggtcggatc gtgatccagg acccgccgca cggcaccggc catgcgctgg
     1201 ctgtcgccgc accggctttg cccgagagcg gtgccgtgct ggtgctctat ggcgacaccc
     1261 cgctcctgac ccgagcgacc ctggagaagc tgctcgagcg acgggccagg gacggggccg
     1321 ccgtgacggt gctgggcatg cgcccgcccg atcctgcggg ctacggccgg ctgcgggccg
     1381 acccggacgg ccgcctccat gcgatcgtcg agcacaagca ttgcgacgca gccctgctcg
     1441 cctccgatct ctgcaatgcc ggcgtgctgg cgctcgccgc cgaccggctg cgccacctgc
     1501 tcgacgagct gccccgccag ccggagaacg gcgagtacta cctgaccgac acggtcggcc
     1561 tcgcggttgc caaaggctgg tcgtgcagcg cggtcgaggg cagctggatc gagggccagg
     1621 gggtcaacac ccaggcccag ctcgccaagg tcgccggctt cttccaggcg cgccggcggg
     1681 ccgagctcat ggagcagggg gtgatcatgg aggccccgga cacggtcatg ctcagcgccg
     1741 acacggcgat cggggcgggt gccgtgatcg agcccttcgt cgtcttccgc ccgggcgtca
     1801 cggtcgagcc gggtgcggtg atccacgccc actcctatct cgagcaggcg cgggtcgggg
     1861 ccggggccga gatcgggccg ttcgcccgac tgcgaccggg tgccgagatc ggcgaccgcg
     1921 ccaagatcgg caatttcgtc gaggtcaaga acacccggct cggcgccggc gcgaaagcca
     1981 gccacctgac ctatctcggc gattgcgagg tgggggcggg cgccaatatc ggtgccggca
     2041 cggtcacctg caactatgac ggattcatga agcaccggac cgtgatcggc gaagaagcct
     2101 tcgtcggctc caacacggcg ctcgtggcgc cggtggtgat cggcgagcgg gtggtggtcg
     2161 gtgccggcag tgtcctcacc gcctacgtgc cggccgatgc gctggcgctg tcgcgtgtgc
     2221 cgcagcgtgt attggagggt gccggagcac ggctcaggac ccgtctcgcc cacgaaaaaa
     2281 tgcgccggaa aagctgacgc agaccgatgc agatgctaca gccgggcgtt gagaggattc
     2341 gtcttgcttg gcagagggag gaccgcgaat gcggcgattg acggctatct gcgcagcctg
     2401 ggttgtcgtg gcggctcttg cctgggcggt ggtgccgaca ttcggcggcg gcgccctggc
     2461 gcagggcctg gccgacgtgc ggaccggcaa cgaggcgttc cgccagggtg actacgccgc
     2521 ggcggtgacg gcctataccg atgcgatccg ctccggtgag ctggtcggcg aggccctggc
     2581 catcacctac aacaatcgcg gcgtggccta tggcgaggcc ggcgatttcg aatcggcgat
     2641 cgccgactac agtcaggctc tggcgctgcg gccgggcgat gccacgacca tcaggaacct
     2701 cagggtcgcc catgtccggc gtggcgaggt cctgttcgct tccggcagct acgatgcggc
     2761 aatagccgat ttcgacgcgg cgatcgagat gcaggccgac cactacctcg cctatctcag
     2821 gcgcggcgag ctttatgccg aacgtggcga gctcgacctc gcccgggcgg acctggagca
     2881 ggcggcggcg ctcaaccccg gcgatcccga gatcatcgcc gcgctcgagc gggccgagag
     2941 ccgcgagccc gcgaccgtgg ccgccgacga gcccggcaag gcgtcgccgc ccgagacggc
     3001 aacggcgtcc gcaccggcgg cgagcgaggc accggtggcc gccgccgagc cggtcgaagc
     3061 tgccgagccg gagccggcag cggcaccggc aaccacggcc gaggccgcgc cgcccgccac
     3121 cgaaacggcc gatagcccgg tcgttgcggc ggtgcccgag cggcccgcaa cccccgagcc
     3181 tgcacccgcc gagccggcgg ccgcggcagc cgatgcgccg gcgaccaccg ccgcagcggt
     3241 gccgactccg gcccccgccc cggcggagtc ccgcgcagcg ggcaatggcg agacctggcg
     3301 ggtggtcagc gcggtcaact atcgggctgg gcccgacaat gacgccgagc ggctcggcac
     3361 ggtcgacgcg ggccgcatgg tcacggtcac tggcgatacc ctcggttgga agcatgtcac
     3421 cctgccgaac ggcgatcagg gctacatcta caagaagtgg ctcgagcccg tgactccctg
     3481 aggcaacagg aacgtgtaaa aaaaactctc ctctttcgcc gcttaatcga atcgcctaaa
     3541 ggcagtgtta accttcgcgt gatagagaag gtggggttgg cggacagcgg gcggcggttc
     3601 cagtcccaac ctcaagttga aatttgagcc cggagaggca tgggtatgct ctgtcgcctt
     3661 gccttggcag tcttccttct ggcgctcggt gccgcgacgg cgtcgccggt gcaggccaac
     3721 agcaagtacg cggccgtcgt cctcgactac gcgaccggcg aggtgctgca ctcgcgccgc
     3781 gccgatgtcg ccctctatcc cgcctcgctc accaagatga tgaccctcta catgctgttc
     3841 gaggcgctcg agcggggcga gctccggctc ggcagcaagc tcgatgtctc gcggcgcgcc
     3901 gccggcatgc cggcgtcgaa gctcggcctc ggggccggct cgaccatcac cgtcgagcag
     3961 gcgatcgagg cgatcgtcgt caagtcggcc aatgacgtgg ccgtggtgat cgccgaggcg
     4021 ctggccggca gcgagccacg gttcgccgat gcgatgaccc gccgggcgcg ggccatcggg
     4081 atgcgtcata ccaccttcaa gaatgcctcg ggcctgccgg atcgccagca gaagagctcg
     4141 gcggccgaca tggcccggct ggcctaccgg ctgatcagcg accatccccg ctactacccc
     4201 tatttcagtc ggcgcagctt ctccttcaac ggcgtcaacc atcgcggcca caatcggctt
     4261 ctggtcagct atgccggcat ggacgggctg aagaccggct acacgcgggc ttcgggcttc
     4321 aatctcgcgg ccacggcgcg gcgcagcggc gagcgtgtca tcgttgtggt gttcggcggg
     4381 cgcagcgccc gcacccgcga caaggaggtc gcccgcctgc tcgatctcgg cttcgagcgg
     4441 gtccgcgagc gccgcccggc gccggcgatc gccctcctgc cgcaggcgaa gcctgggacg
     4501 accatggcgg cggtggccgt cgtcgatacg gctgacgatg ccatagcgcc cgaagcgggg
     4561 cctgtgccgg cattgcggcc cggcacgctg gtcgaggtgc cgggcgtgcg cccggcgccg
     4621 gtcgttgctg tggcccctgc cgtctcggtg ctcgacgcgc tgccgccggc cgagcgcgcg
     4681 gtcatcgtcg cggctgcggc gaacgccccg ttgccggccc ccaaccccca gcgcctcgtg
     4741 gtttccgggg gggccggaac ggctgccacc gggccctttg cggtccaggt cggcgccttc
     4801 cacgactcga atctggcgcg ccgtgccgcc cagacggcgg ccaggctggt gccgcagatc
     4861 ctgatgcagg gtgccatcga cgtcagcccg ctcaagggcc ggcggcgcac cgtctaccgg
     4921 gcgcgggtcg ccggcttcga gcgcgcggtg gcggacgagg cctgccagat gctgcagcag
     4981 catcaccagg agtgcttcgt cgtccaaacg cggggctacg aggtggcggg ccggacctcg
     5041 tgactgcgcc ggccgaagcc cgcttccctc gggccgcgcc tgacccgctc ccggggcatc
     5101 accgccgcgc ttgaccacaa cgtcgcttcc tcactaccgt cccctctctt catgacgacc
     5161 gggcgcggtc ccggtcggct ggtcgaggtg ggaggtgcat ggcgtgggcg agggcgcgga
     5221 tatccggggc ggtcgactgc cggccgccgc ctatgacgag aatttcgccg atctccagcc
     5281 gccgctggcg aggcaggagg ccgtggtcga ggctgcgcgc tgctatttct gctacgatgc
     5341 gccctgcgtc caggcctgcc cgaccggcat cgatatcccg agcttcatcc gcaagatcca
     5401 gaccggcaac ctgaagggtt cggccctcga catcctcgag gccaacatca tgggcggcac
     5461 ctgtgcccgg gtctgcccga ccgaggtgct ctgcgaagaa gcctgcgtgc gcaccgtgca
     5521 ggagggcaag ccggtccgga tcgggcggct gcagcgctac gccaccgatg cgctgttcgc
     5581 caccggcatc cagcccttca gccgggcggc atccaccggc cggcggatcg ccgtggtcgg
     5641 cgccggccct gccggcctcg cctgcgccca tgcgctggcg cggcgcggcc acgacgtcgt
     5701 cgtcttcgag cgccgggcca aggccggcgg gctcaacgag cacggcatcg ctgcctacaa
     5761 gatggcgaac gacttcgccg cgcgcgaggt cgccttcatc ctcggcatcg gcggcatcga
     5821 gctccacctc ggccaggcgc tcggtcgcga gctgacgctc gacgccctgc gccgcgacca
     5881 cgacgccgtc ttcctcggcg tcggcctcgg caccaccaac cgcctgggcc tggcgggcga
     5941 ggagctcgag ggtgtgctcg acgccgtcga cttcatcgag gagctgcggc agagcccgga
     6001 caaggcgacc ctgccggtcg gccgacacat cgtcgtcatc ggcggcggca acaccgcgat
     6061 cgacattgcc gtccagacga agcgcctggg tgccgagacc gtcaccctgg tctatcgccg
     6121 cggcgccggc gaaatggggg cgaccggcca cgagcaggag atcgcccagc tgaacggggt
     6181 gctgatccgc cactgggcgc ggccggtgac gctagaggcc gcgggcggcc gggtcgccgg
     6241 cgtgaccttc gagcacaccc ggctcgatgc gggcggcagg ctcgagggca cgggcgagcg
     6301 ctttaccctc gccgccgacc aggtcttcaa ggcgattggc cagaagctcc tggcggacga
     6361 gctcgccggt agcggcctcg cgatcgaggc cggcaagctt cgggtcgacg acgagctgcg
     6421 cacctcgctc gacggcgtct gggccggcgg cgatgccgtg gccggccagg atctcaccgt
     6481 ggccgcggtc gaggatggca agcgcgccgc ccgctcgatc gaccgggcac ttggcaacta
     6541 ggcagccggg aggcagtcat ggccgatctc cgttgcacca tcgccggtgt gaagtcgccc
     6601 aaccccttct ggctcgcctc cgcaccgccg accgacaagg cctacaacgt ggtccgcgcc
     6661 tttcgcgccg gctggggcgg ggtggtctgg aagacgctcg gcgaggaccc gcccatcgtc
     6721 aatgtcagct cgcgctacgg ggcggtccat ttccgcggcc accgcatggc cggcttcaac
     6781 aatatcgagc tgattaccga tcgcaccctc gaggtgaacc tgcgggagat aacccaggtc
     6841 aagcgcgact ggccggaccg cgccatggtc gtctcgctca tggtgccctg cgaggaggcg
     6901 agctggcagg cgatcctggc ccgggtcgag gcgaccggcg ccgacggggt cgaactcaat
     6961 ttcggctgcc cgcacggcat gagcgagcgc ggcatggg
//
LOCUS       DAOTFI010000243         6980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12867_length_6980_cov_5.985415, whole genome shotgun sequence.
ACCESSION   DAOTFI010000243 DAOTFI010000000
VERSION     DAOTFI010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12867_length_6980_cov_5.985415"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="P5558_19795"
     CDS             complement(<1..828)
                     /locus_tag="P5558_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY26621.1"
                     /translation="MEQTALWFLMAWLDPSLLLMVAAGTFAGIYIGAIPGLSVTMAVS
                     ILISFTFKWPVDDALALIAGIYVGGVYGGARSAILLNIPGAPSAVATAFDGYPLAQRG
                     EAGPAIGVATVMSVVGGLMGVLALALAAPVIANFALLVAPRDYLLLAMIGILLVGSLA
                     GQSLAKGIFAGALGVTIGMVGLDPMTAEGRFTFGSLSLMGGIPYVAAMIGFFGVAEAL
                     YQLRHLDLAPVKQRVGSLLPDWRVVRKLLPLSMRTGAIGVIIGALPGTGGDIAALMAY
                     "
     gene            complement(856..1332)
                     /locus_tag="P5558_19800"
     CDS             complement(856..1332)
                     /locus_tag="P5558_19800"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY26622.1"
                     /translation="MPDEPRREPGELVFSLLLLLASLFLAWQAWTIARFTSLSSAGVM
                     PMLATGTMAAAGLVILGQTARRRPVAEQGAVRRFLAEITPPRLALFAAMIVLYMLALE
                     PAGFLVSSFVFLFAGMGFLHRRSLALSLVVSAASLALIWLVFRHVFAVVLPAGRLF"
     gene            complement(1407..2402)
                     /locus_tag="P5558_19805"
     CDS             complement(1407..2402)
                     /locus_tag="P5558_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY26623.1"
                     /translation="MFQKLLGAATALSLAATVATAQEFPEREMLGVIMWGAGGATDVV
                     ARGVTPAVEEALGKPIVMLNKAGAAGAISVAYVDAAPADGYTLLYGAENPQLHGVMGL
                     SELDYGSFYPVNILGRGVAVVVVQADAPWQSFKELVLDAQARPGEIKMASTGPGGLPY
                     VVAALLNSVAPHEVTAVPFDGEGPGLTALQGGHVDFMPAGISAAAELIKGGQLRALTV
                     VNAESLATFPDVPPITDEFPDMAKYLPWGPFYGVWVRDETPEDVKAKLVDAFKVGAES
                     EAFQTLMANRGNVMMNISGDEADAFLENWQSVTTWVLHDGGATKRSPEEFGIPRP"
     gene            2496..3236
                     /locus_tag="P5558_19810"
     CDS             2496..3236
                     /locus_tag="P5558_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRY26624.1"
                     /translation="MQHRPGGTAKGGKALYGAPVGILMLEARFPRIPGDVGNALSFPF
                     PVLYAVVPGASPDRVVRRRAEGLLDSFIAEGRKLVRQGAEGIVTNCGFLALYQGELAA
                     ALEVPVATSSLLQVPFIERLLSPGRRVGILTIAAGHLSAEHLAAAGVAPDTPIAGTPP
                     DGHFVRAILDDGPALEPLRCEAEVVEAALGLVASAPEVGALVLECTNMCPYSRAVRRR
                     LDLPVFDMLSLIAWFQQGLRPPVHPPFD"
     gene            3347..>3768
                     /locus_tag="P5558_19815"
     CDS             3347..>3768
                     /locus_tag="P5558_19815"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HRY26625.1"
                     /translation="MSREARRGGRGARRAERLGTGLRTLPGVQYRIPVYEVLNEEGLE
                     LIHDASCRILEEVGIEFRDAEACAIWREAGADVQGERVHIPRVLLMDLVGRAPARFTL
                     HARNPERSVEIGGRDAAFAPNYGSPFVRMADGERRYGTL"
     assembly_gap    3769..3868
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3869..4633
                     /locus_tag="P5558_19820"
     CDS             <3869..4633
                     /locus_tag="P5558_19820"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HRY26626.1"
                     /translation="LAPFTLAGANTPASAVATVAQLNAEALAGIAFAQAVRPGAPVIY
                     GNFLATVSMKSGAPMAGTSEIALMNFMIGQLARRYGVPWRSSGLLTGSKICDAQAAYE
                     SAFNMLPILLAGANFVMHCGGWSEAGLTCNLAKFMLDAEQMEMLHRLAQGPRFDDFEA
                     ALGAIREVGPGGHFLGTGHTQAHFQDAFFMSRLGDNNSFEQWQLDGAKDATARGIEAA
                     EAALLSYEQPPLDPAIDEALDAFIKKREAAIPPSFD"
     gene            4703..5716
                     /locus_tag="P5558_19825"
     CDS             4703..5716
                     /locus_tag="P5558_19825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRY26627.1"
                     /translation="MAQARVAWADLAKGLTIVLVVFGHAVDGVNTAVPLEPWLFDALK
                     LPFGQFRMPLFFFVTGLFAAGSVRRAWPEFADRTLLPLVWVFLVWNVLQYAARMTFAG
                     FANHPADPLEILYFSVQPINVTWFLWALILLYALLRVGRDMPPALLAALTASLAATTL
                     PIDHYALEQTSHFAVYFVLGYALAPVMRAWQARPEGRVVLLWLAGYGALCVLLLAGHV
                     REVPVLDLAVRCYGIGLVILGCAWTASRVRVGWLVGVGRYTLPIFVMHTIFTAGARET
                     LLRLGITAEPMLLILAATLAGLVLPLLLHAAARRVGAPWLFQRPAWFRLPAARPATAA
                     AEG"
BASE COUNT          989 a         2223 c         2585 g         1083 t
ORIGIN      
        1 gtaggccatg agtgccgcga tatcgccgcc ggtgcccggc aaggcgccga tgatcacgcc
       61 gatggccccg gtgcgcatcg agagcggcag gagctttcgc accacgcgcc agtcgggcag
      121 caggctgccg acccgctgct tcaccggggc gaggtcgaga tggcgaagct ggtagagcgc
      181 ctcggcgacg ccgaagaagc cgatcatcgc cgccacgtag gggatgccgc ccatcaggct
      241 gagcgagccg aaggtgaagc ggccctcggc ggtcatcggg tcgagcccga ccatgccgat
      301 ggtgacgccg agcgccccgg cgaagatgcc cttggcgagc gactgcccgg cgaggctgcc
      361 gacgagcagg atgccgatca tagccaggag cagatagtcg cgcggcgcca cgaggagggc
      421 gaaattcgcg atcacggggg cggccagggc gagggccagg acacccatga ggccgccgac
      481 caccgacatg acggtggcga caccgatggc cgggccggcc tcgccgcgct gcgccaaagg
      541 atagccgtcg aaggcggtgg cgacggcgct gggtgctccc gggatgttga gcaggatggc
      601 gctcctggcc ccgccataga cgccgcccac atagatcccg gcgatcagcg ccagggcgtc
      661 gtcgaccggc catttgaagg tgaaggagat gaggatggat accgccatcg tcaccgagag
      721 gccgggaatg gcgccgatat agatgccggc gaaggtgccg gcggcgacca tgagcagcag
      781 cgacgggtcg agccacgcca tgaggaacca gagggcggtc tgctccatca ctccgctacc
      841 atccgtgggg caacatcaga agagccggcc ggccggcagc acgaccgcga agacgtggcg
      901 gaagacgagc cagatcaggg cgaggctggc ggcgctgacg acgaggctca gggcgagcga
      961 gcggcggtgc aggaagccca tgccggcgaa gaggaagacg aaggagctga cgaggaagcc
     1021 ggcgggctcg agggccagca tgtagagcac gatcatggcc gcgaagaggg cgagcctggg
     1081 cggcgtgatc tcggcgagga agcggcggac ggcaccctgc tccgccaccg gccggcgccg
     1141 cgcggtctgg cccaggatga cgaggccggc ggccgccatg gtgccggtcg ccagcatcgg
     1201 catgaccccg gccgaggaga gcgaggtgaa gcgggcgatc gtccaggcct gccaggcgag
     1261 gaagaggctc gccaggagga ggagcaggct gaagacgagc tcgccgggct cacggcgtgg
     1321 ctcgtctggc atggaactca tggcgtcagc gagggcggta gccggcgcgg ccccggccgg
     1381 gtctggccgg agccggtcgc tgtcgctcag ggccgtggga tgccgaactc ctcgggcgag
     1441 cgcttggtgg cgccgccgtc atgcagcacc caggtcgtga ccgactgcca gttctcgagg
     1501 aaggcgtcgg cctcgtcgcc cgagatgttc atcatgacat tgccgcgatt ggccatcagc
     1561 gtctggaagg cttcgctctc ggcaccgacc ttgaaggcat cgacgagctt cgccttgacg
     1621 tcttccggcg tctcgtccct gacccagacg ccgtagaacg ggccccaggg caggtatttc
     1681 gccatgtcgg ggaactcgtc ggtgatcggc ggcacgtcgg ggaaggtggc cagactttcc
     1741 gcgttgacca cggtgagtgc tctgagctgg ccgcccttga tgagctcggc cgcggccgag
     1801 atgccggccg gcatgaagtc gacatgcccg ccctgcagcg ccgtgaggcc gggaccttcg
     1861 ccgtcgaacg gcacggcggt gacctcgtgc ggggcgacgg agttgagcag ggccgccacg
     1921 acataaggca ggccgccggg gccggtcgaa gccatcttga tctcgcccgg ccgggcctgg
     1981 gcgtccagga ccagctcctt gaaggactgc cagggcgcat cggcctggac gaccaccacg
     2041 gcgacgccgc ggcccaggat gttgaccggg tagaagctgc cgtagtcgag ctcggagagg
     2101 cccatgacgc cgtgcagctg cgggttctcg gccccataga ggagcgtgta gccgtcggcc
     2161 ggggcggcgt cgacataggc gaccgagatg gcgccggcgg cgcccgcctt gttgagcatg
     2221 acgatgggct tgccgagcgc ctcctcgacg gcgggcgtga caccgcgggc gaccacgtcg
     2281 gtggccccgc cggcacccca catgatgacg ccgagcatct cgcgctcggg gaattcctgg
     2341 gccgtggcga cggtcgcggc gagcgaaagg gcggtggccg ccccgagcag cttctggaac
     2401 atgaatgcct ccctgcgttt ggtctgtcgc cggcttgatg cccgacatgc acgggcgcag
     2461 tctagaggca gtcgccagcg atcggaggga tcggcatgca gcatcggccg ggcgggaccg
     2521 ccaaaggcgg caaggcgctc tatggtgcgc cggtcggcat cctgatgctc gaggcgcgct
     2581 ttccccgcat cccgggcgat gtcggcaacg ccctctcctt cccctttccg gtgctctacg
     2641 cggtggtgcc gggggcctcg ccggaccggg tggtgcggcg gcgggcggag gggctgctcg
     2701 acagcttcat cgccgagggg cggaagctgg tgcggcaggg ggccgagggg atcgtcacca
     2761 attgcggctt cctcgccctc taccagggcg agctggcggc agcgctcgaa gtaccggtgg
     2821 cgacgtcgag cctgctgcag gtgccgttca tcgagcggct gctgtcgccc ggccgccggg
     2881 tcggcatcct caccatcgcg gccgggcatc tgagtgcgga gcacttggcg gcggccggcg
     2941 tcgcccccga cacgccgatc gccgggacgc cgccggacgg gcacttcgtc cgggccattc
     3001 tcgatgacgg gccggcgctc gagcctctgc gttgcgaagc cgaggtcgtc gaggcggctc
     3061 tcgggctcgt ggcaagcgct ccggaggtcg gtgcgctcgt gctggaatgc accaacatgt
     3121 gcccctacag ccgggcggtg cgccggcggc tcgatctccc ggtcttcgac atgctgagcc
     3181 tgatcgcctg gttccagcag gggctccggc cgccggtcca cccgcccttc gactgaccct
     3241 tgtcttgaac gggcaagtcg ctgtcgcctc ggcggcggcg gcgcgatctg gtgccggcta
     3301 tctgaccggg gagaccggcc gtgttgcgcc ggcaggggga acggccatgt ccagagaggc
     3361 acgtcgaggt gggcgcgggg cgcggcgcgc cgagcggctc ggcacggggc tccgcacgct
     3421 gcccggcgtg cagtaccgca ttccggtcta cgaggtgctg aacgaggaag gcctcgagct
     3481 gatccacgac gcctcctgca ggatcctcga ggaggtcggc atcgagttcc gcgacgcgga
     3541 ggcgtgcgcc atctggcgcg aggcgggggc ggacgtgcag ggcgagcggg tgcacatccc
     3601 gcgggtgctg ctcatggacc tcgtcggccg cgcgccggcg cggttcacgc tgcatgcccg
     3661 caaccccgag cggtcggtcg agatcggcgg cagggatgcc gccttcgcgc ccaattacgg
     3721 ctcgcccttc gtgcgcatgg cggacggcga gcggcgctac ggcacgctnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ggcccccttc accctggccg gcgccaacac
     3901 cccggcctcg gccgtggcga ccgtggccca gctcaatgcc gaggccctcg ccggcatcgc
     3961 cttcgcccag gcggtgcggc cgggcgcacc cgtgatctac ggcaatttcc tcgccacggt
     4021 ctcgatgaag tcgggggcgc cgatggccgg gacctccgag atcgcgctca tgaacttcat
     4081 gatcggccag ctcgcgcggc gctatggcgt gccctggcgc tcgtccgggc tgctcaccgg
     4141 ctcgaagatc tgcgacgccc aggcggccta cgaatccgcc ttcaacatgc tgccgatcct
     4201 gctcgccggc gccaatttcg tcatgcactg tggcggctgg tcggaagccg ggctgacgtg
     4261 caacctcgcc aagttcatgc tcgacgccga gcagatggag atgctccacc gcctcgccca
     4321 ggggccgcgc ttcgacgatt tcgaggcagc gctgggtgcc atccgcgagg tcgggccggg
     4381 cgggcatttc ctcggcacgg gccataccca ggcgcatttc caggacgcct tcttcatgag
     4441 ccggctcggc gacaacaaca gcttcgagca gtggcagctc gacggggcga aggacgcgac
     4501 cgcccgcggc atcgaggcgg cggaggcggc gctcctcagc tacgagcagc cgccgctcga
     4561 cccggcgatc gacgaggcgc tcgacgcctt catcaagaag cgcgaggcgg cgatcccgcc
     4621 gagcttcgac tgaggccgtt aacgaattct tcagtcgggg ctgtcagtct cgccgcctgg
     4681 aaacaacggc gggcgggatg ccatggcgca ggcacgggtc gcttgggccg atctggccaa
     4741 ggggctgacg atcgtgctcg tggtgttcgg gcacgcggtg gacggcgtga acacggccgt
     4801 gccgctcgag ccctggctct tcgacgcgct gaagctgccg ttcgggcagt tccgcatgcc
     4861 gctcttcttc ttcgtcaccg ggctcttcgc cgccggcagc gtgcggcggg cgtggcccga
     4921 gttcgccgac cggacgctcc tgccgctcgt ctgggtgttc ctcgtctgga acgtcctcca
     4981 gtatgcggca cgcatgacct tcgccgggtt cgccaatcat ccggccgacc cgctcgagat
     5041 cctctacttt tcggtccagc cgatcaacgt cacctggttc ctctgggcgc tcatcctcct
     5101 ctacgcgctg ctgcgggtcg gccgcgacat gccgccggcg ctgctcgcgg cgctcaccgc
     5161 gtcgctggcg gcgacgacgc tgccgatcga ccactatgcg ctggagcaga cctcgcattt
     5221 cgccgtctat ttcgtgctcg gctacgccct ggcgccggtg atgcgggcct ggcaggcgcg
     5281 gccggagggg cgggtggtgc tgctctggct cgccggctac ggggcgctct gcgtgctgct
     5341 gctggcgggg catgtgcgcg aggtgccggt gctcgatctg gcggtgcgct gctacggcat
     5401 cggcctcgtg atcctgggct gcgcctggac ggcgagccgg gtccgggtcg gctggctggt
     5461 gggcgtcggc cgctacacgc tgccgatctt cgtcatgcac acgatcttca ccgccggcgc
     5521 cagggagacc ctgctgcgcc tcgggatcac ggcggagccg atgctgctga tcctggcggc
     5581 gacgctggcc gggctggtcc tgccgctgct gctgcacgcg gcggcgcggc gggtgggggc
     5641 accctggctc ttccagcgcc ccgcctggtt ccgcctgccg gcggcccggc cggcaacggc
     5701 cgccgccgag ggctgacccg gacggcctgg atggcgtggg cgggctcgat ggcgcgggcc
     5761 agccggattg gggcggactc gatggcgtgg gcgggctgga tggcgtgggc cagctcgatg
     5821 gcgcgggtgg gctggatggc gcgggtgggc tggatggcgc gggtggcggt cctgactttg
     5881 ccgggcggcc gcagcggtgt cccggccggc aacggcgccg ggcgggcggg cttggttggc
     5941 ggcttccgcg gcgtcatgcg gggcggcatg agggaacgct tgataggatt tatagcctag
     6001 ttcgggtggg gtgtcaaggg gtgggcgggt tcgattggtg atttcgggtt ctcgggttgg
     6061 ccggcgcctt cgggaggggt cgggctggcg gaaaaagggc ttggtttcag ggcgttggcg
     6121 ggtctcgggt tcgttggtcg ggggtcgggt tcgatcgtcg gaaaggcggg ttccttcgtc
     6181 ggaatcccgg gttcgatcgt cgatttggtg ggttcgttcg cggcggggtg ttgcggaatc
     6241 cgcgacattt ccgggcggat gtcgcggcgg atgcgccatc acgaatcgtt atgtcgcgga
     6301 atccgcgaca tttgaacggg tgcggttgcg ccagacgcga caacccgagc cggcatgtcg
     6361 cagattccgc gacaacccgg gcgatgcagg cggccgaagg gcaccagacg acgcgcgcgg
     6421 acgggttttc gaggaccggg cataagcgtt cgtgatggcg tcggggcggt tgaggtcgtg
     6481 cccatgacgg cccgcgccag aacgcacgag aatggtctca tgtcccggcc ggctggcctc
     6541 cggccgaagc gatgtggcgg cgaaagggtt ccgaccgcgt ttgggatccg cgggcctcag
     6601 tgttcgttga ccgtggtctt gtggacccga aattccatac gtactcggac ccggtgtggc
     6661 acaggcgcgt catgggtaac tggtacggca aagcggactt tctttcgcac ggcataagcg
     6721 agagcgtcct cggcaagaac gcgctagagt ttcggtcgac cggtgatcac atgtggcgga
     6781 tcggcaagtg ctgttcgaag tttcatacaa ggacatctcg gaactccgag acgaccagct
     6841 cagggagttg gtggctgcgc tctgtcgcgc ggaactctcc ggtgcgggtt tgcccgtatc
     6901 ggcagttacg gccggaggca accatctagc ccgcttcatt catgaggctg cgagtgagtt
     6961 ggcggtcagg cgcgcgtgag
//
LOCUS       DAOTFI010000244         6930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_12986_length_6930_cov_4.412655, whole genome shotgun sequence.
ACCESSION   DAOTFI010000244 DAOTFI010000000
VERSION     DAOTFI010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12986_length_6930_cov_4.412655"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..2716)
                     /locus_tag="P5558_19830"
     CDS             complement(41..2716)
                     /locus_tag="P5558_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26628.1"
                     /translation="MTDLGIGTLTAGDLRRGAVQWRPDLAEFLGSHVASAFEDSIPGM
                     VLRDSMLPARSPMTAEGEFRQSINDPAMQQRISAHLAEHPEKRALSEAEWKASPWYRD
                     GLAFDPWATAERAEARAGMFDLRQWRQHVATAREPGIGESVLAFGAELVGSFADPVNF
                     VPLVGPAWRAAWVAKAATRSAYLMRAAAIGAFEGAAGTALVMPYTLTSNAYVGDDIGF
                     ADVMLDLAIGAALPAAVSGAGGWWSTRGGRSWALDLDPGGASVTPRPLTLDDLARESG
                     GARPAPLDPVATARSLRDIQAAAADIATGRPIDTRGLREPAAPTLGDRLRAAPLDGPE
                     WVLTADNQRFLAAPDGTPDIGRAPRVESDAPTAEELPVRLRRSDIRHLDDDRHRRIAS
                     GLGYADGVELVLDVVQGWTSLHEGHDGRLVLAKRNGNAAVAVVELAKADGGDFWRIVT
                     GGGRAPERLGRELGTRAPTPGSASGQQSSLTSGVGAAGDAPNSAQSPRTGNGAAGAGP
                     QPDAGTNRAEAPGSAPDAPDGAPETIDHPAAHGEDAAAEALATPEIDHIGRAVENARR
                     PSRSPADDGTTRQGEAASGPTRADHELVSDVEAMLDPSDGDLLDVSAGYVSARSAERV
                     RRMTGLDTYDLNLRVNKRTAREIRDRRGPGTEARERGEIPVEAEDYLHLNQVVDQPDR
                     VTRSTDPNGKTILRYEKRMPDGTVYHAEFSHGGSMYLRDFHKRREGGGGDGPGRGGEG
                     PDGRGGPDAGRGAEPDGRGGPDAHDRDGADTGRGGEPGGRPDGHATGRARARDDGGDP
                     PPDGLAGDSAGDGTGDGMGDSLEGAATGGATSAAAETLDPDPDIAAFQVARDAGQVGA
                     EEARAFARADAELERLARLEEAYDMATMCVLRGRG"
     gene            complement(2713..4866)
                     /locus_tag="P5558_19835"
     CDS             complement(2713..4866)
                     /locus_tag="P5558_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26629.1"
                     /translation="MIQIPYQDRFAGKGSAAVSVVPVGAGNANGQAIARGVQVAADQL
                     GQYGRTVVEQEDRTERAKVALAFTQSQERWLTDLEERKRNLAGDGKGFSDGVVADYDE
                     WASGFLTAHGSGNPQVRAWLEERLADQRLGLRARSLDIEGTRRGVFQGQAVEETAKGW
                     ADAAFKAPLDYEEHAAGYNASIDLLDLDPAIDEKLRGEGLEDIALATGLSLAERDPAL
                     AYEQAMNGAPWAKQLSAPKLDSIATKAQAELKRRKVEAEQAAREAEQAARVRMHDAQE
                     GIAARLQDDTARLASGLTPESALTQDEVMEAFGPEKGPAAWQKIERQLAFAETMGRIA
                     GAAPDEQAALLDAARPDPDADGFALAQDRFETLQKAIAADTEQRRKDPAGYVGRVNRP
                     LAEAFEAADTPEAFGRAVTLSLAEQERLGVPAALRRAVPDATVERVAETVQGLAGARD
                     KVARIGAYALGIAEPGPRRAFMADLVKAGLPEGLRFVLPALEAGDEVGAAELLGALEA
                     KITLEGDPKKELRAALEESGETAFETLTAALTGDARPLAEGRERLAAIERLAAARVQA
                     GADPDDAVAHARKVAGGGVARLVDPDLGAITLPGDGDATRIEQGLQRFRHEITEHVDA
                     AVLARMLSPEGQGFEASFARRMGEELLEDMIDGARWLDHPAGGYGLLVTLPDGTTGFL
                     PGADGQAIRLEAAEADKLGGPGWTSFQPDMGSAGP"
     gene            complement(4866..5387)
                     /locus_tag="P5558_19840"
     CDS             complement(4866..5387)
                     /locus_tag="P5558_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26630.1"
                     /translation="MAGPELLLAAAAVSAAAGVMGGVAANSQGKAAQAAADQEAEQAA
                     EAAGIRQAQNSRQEEALRRDQAREAAIDRAGAAESGQGPGGTALELFKLRAIAGERDA
                     LALRYSGELEALGFKRQSQNSLYRGKVAAAAGRNEMIGSFIGAGATMLGGFGDYKVGK
                     QQEAAALAGGAGG"
     gene            complement(5387..5605)
                     /locus_tag="P5558_19845"
     CDS             complement(5387..5605)
                     /locus_tag="P5558_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26631.1"
                     /translation="MAEQVRALDARVALVEAKLGPVETLAATSAVRLEHISKAVDSLL
                     ATVRWVALFVIAGVGSAILNLVINRGGG"
     gene            complement(5637..6113)
                     /locus_tag="P5558_19850"
     CDS             complement(5637..6113)
                     /locus_tag="P5558_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26632.1"
                     /translation="MRVRSFRAADLVLLTAPAGPGLQAGQAYLAPGLTPALGSSLVHA
                     GPAFTVATDDGRPIGCIGLVEQWEGRALAWAVLAGDAGRVLVGITRAVRRFLDLGRYR
                     RIEAAVPVGFAAGHRWARLLGFTLEAPVMRSWSAGRDFALYALIAPENRAEQGAGG"
     gene            complement(6116..>6930)
                     /locus_tag="P5558_19855"
     CDS             complement(6116..>6930)
                     /locus_tag="P5558_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26633.1"
                     /translation="TLGDGSLVSFTYDPAQEVVAWARHPLTARDGLEHPAGPQAIQVQ
                     TIPGSPGDDGADEVWLLMAELAGYYTPPGSPPQPWFASTLHRLARPHRPGDDRAAAVY
                     LDRSLTYAGPPTTTLGGLGHLEGSTVQVLADGAAHPARVVAGASITLDRSVTRATVGI
                     PAPSLWRSMRLESGRKAGTSQAGFMRVVAATFRLLEAAGVRVGGHFGRKVSLDRREPG
                     DVPGTPPALVTGDRRIRWPAGWRREQRIEIEATGALPATIEAVFPTINVSEG"
BASE COUNT          813 a         2683 c         2493 g          941 t
ORIGIN      
        1 cccgtcatcc ccggcccccc gatcatcccc ggccccccga tcatccccgg ccccgaagca
       61 cgcacatggt cgccatgtcg taggcttcct cgagccgggc caggcgctcg agctcggcat
      121 cggcacgggc gaaggcccgc gcttcctcgg cgccgacctg gccggcgtcg cgcgccacct
      181 ggaaggcggc gatgtcgggg tccgggtcga gtgtctcggc cgcggcggac gtggcgccgc
      241 ccgtcgctgc cccttcgagg ctgtcgccca tcccgtcgcc ggtcccgtcg ccggcactgt
      301 cgccggcaag accgtccggc ggcgggtcgc cgccatcgtc gcgggcgcgc gcccggccgg
      361 tcgcgtggcc gtccgggcga ccgccgggct cgccgccccg cccggtgtcg gcgccgtcgc
      421 gatcatgggc gtcggggcca ccgcgaccgt cgggctcggc accccgcccg gcgtcggggc
      481 caccccggcc gtccggccct tcaccgcctc tccccggccc gtctccaccg ccgccctccc
      541 ggcgcttgtg gaagtcgcgg aggtacatcg agccgccgtg ggaaaattcg gcatggtaga
      601 cggtgccgtc cggcatccgc ttctcgtagc gcaggatcgt cttgccgttc ggatcggtcg
      661 agcgcgtcac ccggtcgggc tggtccacca cctggttcag gtggagatag tcctcggcct
      721 cgacggggat ctcgccccgc tccctggcct cggtgcccgg accgcggcgg tcgcggattt
      781 cccgggcggt cctcttgttg acgcgcaggt tcagatcgta ggtgtcgagg ccggtcatcc
      841 gccggacccg ctcggcggat cgagccgaga cgtagccggc cgagacgtcg agaaggtctc
      901 catccgaagg gtcgagcatg gcctcgacat cgctcaccag ctcgtggtcg gccctggtcg
      961 ggccgctcgc cgcctcgccc tgccgcgtcg tcccgtcgtc cgccggcgag cgtgacggcc
     1021 ggcgggcgtt ctcgaccgcc cggccgatat ggtcgatctc gggcgtggcg agcgcctcgg
     1081 ccgcggcgtc ctcgccatgc gccgccgggt ggtcgatggt ctcgggcgca ccatcgggtg
     1141 cgtcgggcgc actcccgggc gcctcggcgc gattcgtgcc cgcgtccggt tgcggcccgg
     1201 cgccggcggc gccatttccc gttcggggac tctgagccga gtttggtgcg tcgccggcag
     1261 ctccgacgcc cgaagttaat gagctctgct ggccggaagc ggagccgggg gtaggcgccc
     1321 tggtccccag ctcacgaccc aagcgttcgg gcgcgcggcc gcctcccgtg acgatccgcc
     1381 agaaatcccc gccgtcggct ttcgccagct cgaccacggc cacggccgcg ttgccgttgc
     1441 gtttggcgag caccagccgg ccgtcgtgcc cttcgtgcag gctggtccag ccctgcacca
     1501 cgtcgagcac cagctcgacc ccatccgcgt agccgagacc gctcgcgatg cggcgatgcc
     1561 tgtcgtcgtc gaggtggcgg atatcgctcc gccgcaggcg caccggcagc tcctcggccg
     1621 ttggcgcgtc gctctcgacc cgtggcgccc ggccgatatc gggcgtgccg tccggggcgg
     1681 cgaggaagcg ctggttgtcg gcggtgagca cccattcggg gccgtcgagc ggcgccgccc
     1741 gcagccggtc gccgagcgtc ggcgccgcag gctcccgcag cccgcgggtg tcgatcggcc
     1801 ggcccgtggc gatgtcggcg gcggcggcct ggatgtcgcg caggctccgc gcggtggcga
     1861 cgggatcgag cggcgccggc cgggcgccgc cgctctcgcg cgccaggtcg tcgagggtca
     1921 gcgggcgggg cgtgacgctc gccccgcccg ggtcgagatc gagcgcccag gagcggccgc
     1981 ccctcgtgct ccaccagccg ccggcgccgg agacggccgc cggcagggcg gcgccgatgg
     2041 cgaggtcgag catgacgtcg gcgaagccga tatcgtcgcc gacataggcg ttgctggtga
     2101 gcgtgtaggg catgacgagc gccgtgccgg cggcgccctc gaaggcgccg atcgcggcgg
     2161 cgcgcatgag ataggcgctc cgggtcgcgg ccttggcgac ccaggcggcg cgccaggccg
     2221 ggccgaccag cggcacgaag ttgaccggat cggcgaacga gcccaccagc tcggcgccga
     2281 aggcgagcac gctctcgccg atgcccggct cgcgggcggt ggcgacatgc tggcgccatt
     2341 ggcgcagatc gaacatcccg gcccgcgcct cggcccgttc ggcggtggcc cagggatcga
     2401 aggcgagccc gtcgcgatac cagggcgagg ccttccactc cgcctcgctc agcgcccgct
     2461 tctcggggtg ctcggcgaga tgcgcgctta tccgctgctg catcgccggg tcgttaatgc
     2521 tctggcggaa ctcgccctcg gcggtcatcg gcgagcgcgc cggcagcatg ctgtcgcgca
     2581 gcaccatgcc ggggatcgaa tcctcgaagg cggaagcgac gtgcgagccc aggaactcgg
     2641 cgaggtccgg gcgccactgc accgcacccc ggcggagatc gccggccgtc agcgtgccga
     2701 tgccgagatc cgtcatggtc ccgccgagcc catgtcgggc tggaagctcg tccagcccgg
     2761 cccgccgagc ttgtccgcct cggcggcctc gaggcggatc gcctggccgt cggcgccggg
     2821 caggaagccg gtggtgccgt ccggcagggt gaccaggagc ccgtagccgc cggccggatg
     2881 gtcgagccag cgggcgccgt cgatcatgtc ctcgagcagc tcctcgccca tgcgccgggc
     2941 gaagctcgcc tcgaatccct ggccctcggg cgagagcatc cgcgccagga cggccgcgtc
     3001 gacatgctcg gtgatctcgt ggcgaaagcg ctggagcccc tgctcgatcc gggtggcgtc
     3061 gccgtccccg ggcagggtga tcgccccgag atcggggtcg acgagccgcg ccacgccgcc
     3121 gcccgccacc ttgcgggcat gcgccacggc gtcgtcggga tcggcgccgg cctggacccg
     3181 agccgcggcc agccgctcga tcgccgccag ccgctcgcgc ccctcggcga gcggccgcgc
     3241 gtcgccggtc aaggcggcgg tgagcgtctc gaaggccgtc tcgccgctct cctcgagggc
     3301 ggcgcgcagc tccttcttcg ggtcgccctc gagcgtgatc ttggcctcga gcgccccgag
     3361 cagctccgcg gcaccaacct cgtcgccggc ctcgagcgcc ggcaggacga agcgcaggcc
     3421 ctcgggcagc ccggccttca cgaggtcggc catgaacgcc cggcgcggcc cgggctcggc
     3481 gatgccgagc gcgtaggcgc cgatccgggc caccttgtcc cgggcgccgg cgagcccctg
     3541 gactgtctcg gcgacccgct cgacggtggc gtcgggcacg gcgcggcgca gcgccgccgg
     3601 cacgccgagc cgctcctgct cggccagcga gagcgtcacc gcccggccga acgcctcggg
     3661 cgtgtcggcc gcctcgaagg cctcggcgag cggccggttg acccggccga catagcccgc
     3721 cgggtccttc cgccgctgct cggtgtcggc ggcgatcgcc ttctggagcg tctcgaaccg
     3781 gtcctgggcg agagcgaacc cgtcggcatc cgggtccggc ctcgccgcgt cgagcagcgc
     3841 cgcctgctcg tccggcgcgg caccggcgat ccggcccatg gtctcggcga aggcgagctg
     3901 tcgctcgatc ttctgccagg cggccggccc cttctccggc ccgaaggctt ccatcacctc
     3961 gtcctgggtc aaggcgctct cgggcgtcag cccggaggcg agccgggccg tgtcgtcctg
     4021 gagccgggcc gcgatcccct cctgggcgtc gtgcatccgc acgcgcgccg cctgctcggc
     4081 ctcgcgcgcc gcctgctccg cctcgacttt ccgccgcttg agctcggcct gcgccttggt
     4141 ggcgatgctg tcgagcttgg gcgccgagag ctgcttggcc cagggcgcgc cgttcatcgc
     4201 ctgctcgtag gccagcgccg gatcgcgctc cgccagcgac agaccggtgg ccagggcgat
     4261 gtcttccagc ccctcgccgc gcagcttctc gtcgatcgcc gggtcgagat cgaggagatc
     4321 gatcgaagcg ttgtagccgg cggcgtgctc ctcgtagtcc agaggcgcct tgaaggccgc
     4381 gtcggcccat cccttggcgg tttcctcgac cgcctggccc tggaagacgc cgcgccgcgt
     4441 gccctcgata tcgagcgagc gggcgcgcag cccgagccgc tggtcggcca gccgctcctc
     4501 gagccaggcg cgaacctgcg ggttgccgct gccgtgcgcc gtgaggaagc ccgaggccca
     4561 ctcgtcatag tcggcgacca cgccgtcgga gaagcccttg ccgtcgccgg cgaggttgcg
     4621 cttgcgctcc tcgagatcgg tcagccagcg ctcttgggac tgcgtgaagg cgagcgccac
     4681 cttggcccgc tcggtccggt cttcctgctc gaccaccgtg cggccgtact ggccgagctg
     4741 gtcggccgcc acctggacgc cgcgggcgat cgcctggccg ttggcgttgc cggcgccgac
     4801 cggcacgacc gacaccgccg ccgacccctt gccggcgaac cggtcctgat agggaatctg
     4861 gatcatcagc cgcccgcccc gccggcgagc gcggccgctt cctgttgctt gcccaccttg
     4921 tagtcgccaa agccgccgag catggtggcc cccgccccga tgaacgagcc gatcatctcg
     4981 ttgcggccgg cggccgccgc gaccttgccg cggtagaggc tgttctggct ctggcgcttg
     5041 aagccgagcg cctcgagctc gcccgagtag cgcagcgcca gcgcatcccg ctcgccggcg
     5101 atcgcccgca gcttgaagag ctcgagggcg gtgccgcccg gcccctgccc gctttcggca
     5161 gcgccggcgc ggtcgatcgc cgcctcgcgc gcctggtcgc ggcgcagggc ttcctcctgg
     5221 cggctgttct gcgcctggcg aatgccggcc gcttcggccg cctgctccgc ttcctggtcg
     5281 gccgccgctt gcgccgcctt gccctggctg ttggcggcga caccgcccat caccccggcc
     5341 gcggccgaga ccgccgccgc cgccagaagt agctccggcc cggccatcag ccgccccccc
     5401 gattgatgac gagattgagg atcgccgagc ccacgccggc gatcacgaag agcgccaccc
     5461 accgcaccgt cgcgagcagc gagtcgaccg ccttggaaat atgctcgagc cgcaccgccg
     5521 aggtggcggc cagggtctcg accgggccga gcttggcctc gaccagcgcc acccgggcat
     5581 cgagcgcccg cacctgttcc gccatcaccg cggccgctcc cgccggcgcc gccacgtcaa
     5641 ccaccggcgc cctgttcggc cctgttctca ggcgcgatca gggcataaag cgcgaagtcg
     5701 cggccggccg accacgagcg catcaccggc gcctcgagcg tgaagccgag cagccgcgcc
     5761 caccggtggc cggcggcgaa gccgaccggg acggccgcct cgatccgccg ataccggccc
     5821 agatcgagga agcgccgcac cgcccgcgtg atgccgacca gcacccggcc ggcatcgccg
     5881 gcgagcacgg cccaggccag cgcgcggcct tcccattgct cgaccagtcc gatacagccg
     5941 atggggcggc cgtcatccgt ggcgaccgtg aaggccggcc ccgcgtggac gagggacgag
     6001 ccgagcgccg gcgtcagacc cggcgcgaga taggcttgcc ccgcctgaag cccggggccg
     6061 gcaggcgccg tgagcagcac caggtcggca gctcggaagg accggacgcg catcctcagc
     6121 cctcgctcac gttgattgtc gggaacacgg cctcgatcgt ggccggcagg gcgccggtcg
     6181 cctctatctc gatccgctgc tcgcggcgcc agccggccgg ccagcgaatg cgccggtcgc
     6241 cggtgacgag cgccggcggc gtccccggca cgtcgcccgg ctcgcgccga tcgagcgaca
     6301 ccttgcggcc gaaatgcccg ccgacccgca ccccggccgc ctcgagcagc cggaaggtgg
     6361 ccgccaccac ccgcatgaag ccggcctggc tggtccccgc cttgcgcccg gattcgagcc
     6421 gcatggagcg ccagagcgac ggcgccggga tgccgaccgt ggcccgcgtc accgaccggt
     6481 cgagcgtgat cgaggcaccg gcgaccaccc tggccggatg cgccgcgcca tcggcgagca
     6541 cctggacggt ggagccctcg aggtggccga gcccgccgag cgtggtggtc ggcggcccgg
     6601 cataggtcag cgaccggtcg agatagacgg cggccgcgcg atcgtcgccc ggccggtgcg
     6661 gccgcgccag gcggtgcagg gtgctggcga accacggttg aggaggcgag cccggcggcg
     6721 tgtagtagcc ggcgagctcc gccatgagca gccacacctc gtcggcgccg tcgtcgccgg
     6781 gcgagcccgg aatcgtctgc acctggatcg cctgcggccc ggccggatgc tcgaggccgt
     6841 cgcgcgcggt gagcgggtgc cgcgcccagg ccaccacctc ttgcgccgga tcataggtga
     6901 acgacaccag cgacccgtcg ccgagcgtgc
//
LOCUS       DAOTFI010000245         6870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13127_length_6870_cov_7.184593, whole genome shotgun sequence.
ACCESSION   DAOTFI010000245 DAOTFI010000000
VERSION     DAOTFI010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13127_length_6870_cov_7.184593"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..1178
                     /locus_tag="P5558_19860"
     CDS             387..1178
                     /locus_tag="P5558_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26634.1"
                     /translation="MLQQNWRDRLGPAAFGADGRLVDWRSGLLPCLRGLRTAEAMRLF
                     DPVRLRQPESAAAMALNSFLVWRERPRDLRLGERNGFTELRFAARCPTGVRGTPPYLD
                     LIAANGEALVTVTARGPEYLARKPSRLARAYADISLPEAMQGWQALLQDLLDEPGRFR
                     HVDAASLVKNAIGIFRTFPGHRATLLYLFWEPESAEALDFHRHRVELQTILQQVDGSA
                     VTLQAQSFAELWHDWERRFDTPWLRAMVAELRERYAVATPPLSAL"
     gene            1266..2288
                     /locus_tag="P5558_19865"
     CDS             1266..2288
                     /locus_tag="P5558_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HRY26635.1"
                     /translation="MAENGRTYFDRQEVREPGERERSIFHALPGLIRHAIDNAPFFRE
                     QLAGVEPDSVTSRRALARLPVARKAALGDRQRRQPPFGGLTATATGRLAHLFATSGAT
                     PGPVYHPEGGRSDYWRFARAMYAAGFSRGDLVLNCFSYHLRPSGFMADFGARALGCPV
                     IPAGPDDPDRQVRIIAELAPVAFAGRPSVLAALLERAAKLKIGIGSLKKALLSGESTS
                     EAERIALGKQHGIAAFQCYATADLGLVAYESTGRGGLIVDESVIVEIVRPGTGDPVPP
                     GKTGEVLVTAFNPDYPLIRFATGDLAAVVPGESSCGRTNMRLSDRLGRVEPAEAGAGT
                     GRRAGA"
     gene            2275..2928
                     /gene="ccmA"
                     /locus_tag="P5558_19870"
     CDS             2275..2928
                     /gene="ccmA"
                     /locus_tag="P5558_19870"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HRY26636.1"
                     /translation="MPAPDATREAAAPLLEAAGLACRRGGRLLLEAVDWRLAPGDALL
                     LKGANGSGKSSLLRLLAGFLRPAGGRLLHGGRDVFADLARYRAALHHLGYQDALKPAL
                     TVAENLEAAAALLGGERGRLAAALDAFALGGLAATPVRFLSSGQRRRAALARLAAVPR
                     PIWLLDEPGIGLDRRNRDALEALIDRHRGGGGIAVVASHGDVALARPLVLDLDGAGA"
     gene            2925..3593
                     /gene="ccmB"
                     /locus_tag="P5558_19875"
     CDS             2925..3593
                     /gene="ccmB"
                     /locus_tag="P5558_19875"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRY26637.1"
                     /translation="MTGALMALVRRDLHLAWRSPAETLLGVVFFLVALALFPLGVGAS
                     PDILARIGAGVIWVLALLAVLLTLDRLFQHDHEDGSLDLLLLAPLPLELAVLAKCAVH
                     WLTSGLLLVVASPILALLMNLPADAFWALPLALLLGTPSLTLIGAIGAALLLGSRRGA
                     VLTALLVLPLYIPVLIFGVAAVDGVVLGLGARAPFLILGAIQLAALALAPLAIAAALR
                     LASE"
     gene            3774..4640
                     /locus_tag="P5558_19880"
     CDS             3774..4640
                     /locus_tag="P5558_19880"
                     /inference="COORDINATES: protein motif:HMM:NF021536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HRY26638.1"
                     /translation="MVVLNQRSMAYQAVRAMTDNHIGAVLVSQPRGGLAGILTDRDLA
                     LAVLGGELDPKTTPVGEVMSEDVVTCDIGADLGEVVRLMRGHGVRRIPLMEGDRPVGL
                     VTFDDLVLDGSVGLDALRGIVEAQLEVEAPHKPAGMLHPQALATTQGRARALMRAKSR
                     AESTYNRMVGAVAEASGQHRDPAERALLLTLCMLCRRLSPEEAQHLVAQLPSLLQPQL
                     DGCLDGPDRAVTTRAIEDKLAKSLGLEPAAAGACLRAVMKVIARSVSPGQIAEVRGQL
                     PEEMKALLPLAT"
     gene            4680..5828
                     /locus_tag="P5558_19885"
     CDS             4680..5828
                     /locus_tag="P5558_19885"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26639.1"
                     /translation="MDALEQDYAAVLLADHEPPCLSLYQPTYRHHPENRQNPTRFFNL
                     VKELEASLRQKYGFRETGPLLAPFHALMEDQDFWKSTLDGLAVLRTADIFKVYRLQRP
                     VAELAVAADSLHTKPLIRILQSADRFQVLGLDRHGIRLFEGNRDAVDEIEPAPGVPRT
                     LVDALGPERDEPQRTNRVHSATVPGARTRHGTDVRQDKVANETARFFRAVDRAVEEHH
                     SRPSAMPLLLAALPEHHHDFRAVSRNPFLMAEAIDVHPDDLSLETLRERAWQLVQPYY
                     LQRLGDLTERFGAARSSGRGAGDLAEIAVAAAAGRIDTLLLEADRVIPGTFDAGTGTV
                     AFGELEHPAVDDLLDDLGERALRAGGDVVIVPAERMPTDTGAAAIFRY"
     gene            6097..6819
                     /locus_tag="P5558_19890"
     CDS             6097..6819
                     /locus_tag="P5558_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HRY26640.1"
                     /translation="MHALANPLRFQKLTDAILPWVAWPAAVLAPIAFYWALVIAPADY
                     QQGEAYRIIFVHVPAAWMASFAYAAMAAASFVALVWRHPVAEVSARAIAPIGAVFTAI
                     ALFTGAVWGKPMWGTWWVWDARLTSVLILFFLYLGYMALHDAFEDPARGARAASILAL
                     VGAVNLPIIRFSVDWWSTLHQPATILRAGGPAMDPSMLYPLLLMILVAKLYMIAVMIL
                     RIRLQLTERKIQNLRLAAEGRG"
BASE COUNT          899 a         2476 c         2426 g         1069 t
ORIGIN      
        1 gctgctggcg cgcgccgatc gggcgctcta tgacgccaag cgcaccggcc gcaaccggat
       61 cgccgtctgc cacggggtcg atgccgcgtc gaccgaggcg gcgatcgaga agctcggccg
      121 cgtcgccctg ccgctcaagg gctgatcgtc gtccgagggc gaattgatcg gggccgcggc
      181 gccgagcctt cccgctcact tgacggtccg caacattacc tgcgagatgg cccgattggg
      241 tcggcgagcc gacgcggccc gtcgtgtcgg ccatcgtcga ccgcaacgag aagcgcgccg
      301 gggcagggtc cgcaggaccc gatcaacgcg gtgtgccagg gtggcgatgc ggtatgcgga
      361 cgtctttggc gcagcgatac atcggcatgc tccagcagaa ctggcgcgac cggttgggac
      421 cggcggcttt cggcgccgat ggcaggctgg tcgactggcg ctccggcctg ctgccatgcc
      481 tgcgcggcct gcgcaccgcc gaggcgatgc gtctcttcga tccggtccgg ttgcgccaac
      541 ccgaatccgc ggcggcgatg gcgctgaaca gctttctcgt ctggcgcgag cggccgcgcg
      601 acctcaggct cggcgagcgc aacggcttca ccgagctgcg cttcgccgca cgctgcccga
      661 ccggggtacg cggcacgcca ccctatctgg acctgatcgc cgccaacggc gaggcgctgg
      721 tgaccgtgac ggcgcgtggg cccgaatacc tggcgaggaa gccgtcgcgt ctggcgcggg
      781 cctatgccga catctcgctg ccggaggcga tgcagggctg gcaggcgctg ctgcaggatc
      841 tgctcgacga gcccggccgt ttccgccacg tcgatgcggc ctccctggtc aagaacgcca
      901 ttggcatctt tcgcaccttt cccgggcatc gggccacgct gctctatcta ttctgggagc
      961 cggagtcggc ggaggcgctc gacttccatc ggcaccgggt ggagctgcag accattctcc
     1021 agcaggtcga cggctcggcc gtgaccctgc aggcccagag cttcgccgag ctctggcacg
     1081 actgggagag gcggttcgac acgccctggc tgcgggcgat ggtcgcagag ctgcgtgagc
     1141 gctatgccgt ggccactcca cccctctctg ctctatgact ccccgaggat cgccgttggc
     1201 gggtcgcccg tgcggcgccg gcccgggcgg tcgatgccgg gcaagcggac gggagacgga
     1261 ccgccatggc ggagaacggg cgcacttatt tcgaccggca ggaggtgcgt gagccgggcg
     1321 agcgcgagcg ttcgatcttc catgccctgc ctggcctgat ccgccatgcc atcgacaacg
     1381 cacccttctt tcgcgagcag ctcgccgggg tcgagccgga ttcggtcacc agccggaggg
     1441 cgctcgcccg gttgccggtg gcgcgcaagg cggcgctcgg cgaccgccag cgccggcagc
     1501 cgccctttgg cggcctgacc gccaccgcga ccgggcgcct cgctcacctg tttgccacgt
     1561 cgggggccac ccccgggccg gtctatcatc ccgagggcgg gcgctcggac tactggcgct
     1621 tcgcccgcgc catgtatgcg gccggcttca gccgcggcga cctggtgctc aactgcttct
     1681 cctaccattt gcggccgtcc ggcttcatgg ccgatttcgg cgcccgggcg ctgggctgcc
     1741 cggtgatccc ggccggcccg gacgaccccg accggcaggt gcggatcatc gccgagctgg
     1801 cgccggtcgc cttcgccggg cggccctcgg tgctcgccgc cctgctcgag cgggcggcga
     1861 agctcaagat cggcatcggc tcgctgaaga aggcgctgct ctccggcgag tcgaccagcg
     1921 aggcggagcg gatcgccctc ggcaagcagc acggcatcgc cgccttccag tgctacgcca
     1981 ccgccgatct cggcctcgtc gcctatgaga gcaccgggcg cggcgggctg atcgtcgacg
     2041 aatcggtcat cgtcgagatc gtccggccgg gcaccggcga cccggtgccg ccgggcaaga
     2101 ccggcgaggt gctggtcacc gccttcaacc ccgactaccc gctgatccgc ttcgccaccg
     2161 gcgatctcgc cgccgtcgtc ccgggcgaga gcagttgcgg gcgcaccaac atgcgccttt
     2221 ccgaccggct cggccgggtc gagccggccg aggctggagc gggcaccggc cggcgtgccg
     2281 gcgcctgacg ccacccggga ggccgccgca ccgctgctgg aggcggccgg cctcgcctgc
     2341 cggcggggcg gccggctgct gctcgaggcg gtcgactggc ggctggcgcc gggtgacgcc
     2401 ctgctgctca agggtgccaa tggcagcggc aagtcgagcc tgctccggct gctcgcgggc
     2461 ttccttcgcc cggccggcgg ccggctcctg catggcggcc gcgacgtctt cgccgatctc
     2521 gcccgctatc gcgccgcgct gcatcatctg ggctaccagg acgcgctcaa gccggccctg
     2581 accgtcgccg agaacctcga ggcggccgcg gccctgctcg gtggcgagcg cggccggctg
     2641 gcagcggcgc tcgacgcctt cgcgctgggc ggtctcgccg ccacgcccgt gcgcttcctc
     2701 tcgtcgggcc agcggcggcg cgcggcgctg gcccggctcg ccgccgtgcc ccggccgatc
     2761 tggctgctcg acgagcccgg catcggcctc gaccggcgca accgcgacgc cctcgaggcg
     2821 ctgatcgacc ggcatcgggg agggggcggg atcgccgtgg tggcgagcca tggcgacgtg
     2881 gcgctggcgc ggccgctggt gctggacctc gacggcgccg gcgcatgacg ggtgccctca
     2941 tggcgctcgt gcgccgcgac ctccacctcg cctggcgctc gccggccgag accctgctcg
     3001 gtgtggtctt ctttctcgtg gcgctcgcgc tctttccgct gggggtcggc gcctcgccgg
     3061 atatcctggc ccggatcggc gcgggggtga tctgggtcct ggcgctgctc gccgtgctcc
     3121 tgacgctgga ccgcctcttc cagcacgacc acgaggacgg ctcgctcgac ctcctgctgc
     3181 tggcaccgct gccgctggag ctcgccgtgc tcgccaagtg cgccgtgcac tggctgacca
     3241 gcgggcttct cctggtcgtc gcctcgccga tcctggccct gctcatgaac ctgccggccg
     3301 acgccttctg ggcgctgccg ctcgccctgc tgctcggcac gccgagcctg acgctgatcg
     3361 gcgccatcgg ggcggccctc ctgctcggca gccggcgcgg ggcggtgctc acggcactcc
     3421 tcgtcctgcc gctctacatc cccgtcctga tcttcggcgt ggccgccgtc gacggcgtcg
     3481 tgctcggcct cggcgccagg gcgccgttcc tgatcctcgg cgccatccag ctcgccgccc
     3541 tggcgctggc accgctcgcg atcgcggcag cgctcaggct ggcgtcggaa tagcgcctcg
     3601 ggcccggatc ggtgtcaaaa ggtggcgctg gccttgccaa acggcggccg aaaggcgaga
     3661 ctcgagccgg actgccctgg aacagggtaa atgccgacga aatagaggcg ctcatcgctc
     3721 cgcctcgcca tgaggggatt gaaatgtcgc tcgagaaatt ccgcagaaca cgcatggtcg
     3781 ttctcaacca gcggtcgatg gcctatcagg cggtccgggc gatgacggac aaccacatcg
     3841 gtgccgtgct ggtgagccag ccgcgtggcg ggcttgcggg cattctcacc gatcgcgacc
     3901 tggccctggc ggtgctcggc ggcgagctcg atcccaagac gactccggtg ggcgaggtga
     3961 tgtccgagga cgtggtcacc tgcgacatcg gcgccgatct cggcgaggtg gtccgtctga
     4021 tgcggggtca cggtgttcgc cgcatcccgt tgatggaagg cgaccggccc gtcgggctgg
     4081 tgacgttcga cgatctcgtg ctcgacggtt ccgtcggcct cgacgccctg cgcggcatcg
     4141 tcgaggccca gctcgaggtc gaggcgcccc acaagcccgc cggcatgctg cacccgcagg
     4201 cgctggccac gacgcagggg cgcgctcgcg ccttgatgcg ggccaagtca cgggccgagt
     4261 ccacctacaa ccgcatggtg ggcgcggtcg ccgaggcgag cgggcagcac cgcgaccccg
     4321 ccgagcgcgc gctcctgctc accctctgca tgctgtgccg gcggctctcg ccggaggagg
     4381 cgcagcacct cgtcgcgcag ttgccctcgc tgctccagcc gcagctcgac gggtgcctgg
     4441 acgggccgga ccgggcggtg acgacgcggg ccatcgagga caagctcgcg aagtcgctcg
     4501 ggctggaacc cgcggccgcc ggcgcgtgcc tgcgggcggt gatgaaggtg atcgccagga
     4561 gcgtctcgcc cggccagatc gccgaggtcc gcggccaatt gccggaggag atgaaggcct
     4621 tgctgccgct ggccacatga gcgccgcagc gccgatttcg aacgtgaagg atactgacca
     4681 tggatgcact cgagcaggac tacgccgcgg tgctgctggc ggaccacgag ccgccctgcc
     4741 tctcgctcta ccagccgacg taccgtcacc atccggagaa ccggcagaat ccgacccgct
     4801 tcttcaacct cgtgaaggag ctggaagcct cgctgcgcca gaaatacggc tttcgcgaga
     4861 ccgggccgct cctggcgcct ttccacgcct tgatggagga ccaggacttc tggaagagca
     4921 ccctcgacgg gctggcggtt ctgcggacgg cggacatctt caaggtctac cggctgcagc
     4981 ggccggtggc cgagctggcc gtcgcggccg acagcctgca caccaagccg ttgatccgca
     5041 tcctgcagtc ggccgatcgc ttccaggttc tcggcctcga ccggcatggg atccggctgt
     5101 tcgagggcaa ccgggatgcc gtcgacgaga tcgagccggc gccgggggtg ccgcgcacgc
     5161 tcgtggatgc gctggggccg gaaagggacg agccgcagcg cacgaaccgg gtccatagcg
     5221 ccacggtgcc gggcgcgcgg acgcgtcacg gaacggatgt gcgacaggac aaggtcgcga
     5281 acgaaacggc gcgcttcttc cgcgcggtcg atcgcgcggt ggaggagcat cattcacggc
     5341 cgagcgcgat gcccttgctg ctggcggcgc tgccggagca ccaccatgat ttccgcgcgg
     5401 tcagccgcaa cccgttcctc atggcggagg cgatcgacgt tcatcccgat gacctgtcgc
     5461 tcgagacgtt gcgggagcgc gcctggcagc tcgtccagcc ctactatctc cagcgcctgg
     5521 gcgacctgac cgagcggttc ggcgcggcga ggtcgagcgg ccggggcgcc ggcgatctgg
     5581 ccgagatcgc cgtggcggcc gcagccggcc gcatcgacac gctgctgctc gaggcggatc
     5641 gcgtgattcc cggcaccttc gatgccggca ccggcaccgt ggcgttcggc gagctcgagc
     5701 atcccgccgt ggacgatctg ctcgatgatc tgggcgagcg ggcgctgcgg gccggcggtg
     5761 acgtggtgat cgtgccggcc gagcgcatgc cgaccgacac gggagccgcg gcgatctttc
     5821 ggtactgacc gggcggaagg gaatccggcg ccgcccgggt gctgccgctt gtcgcggggg
     5881 cacgcggcag tggaagcgat tttccccagc cggattccgc cctgggagcc tgtccgaaga
     5941 atggccatcg cctgcacgcg ccattcccgg ctttgggcga ggagcggcga cgaaggcgat
     6001 gcctcagcat cggcgaggcg gcgcgacgcg ggcctcgccc gccagaaagc gatgcaggcg
     6061 atggccattt ctcggacagg ctcctaaaac ccgctcatgc atgcgctcgc caacccgctc
     6121 cgcttccaga agctcaccga cgccatcctg ccctgggtcg cctggccggc ggccgtgctg
     6181 gcgccgatcg cgttctactg ggcgctcgtg atcgcccccg ccgactacca gcagggcgag
     6241 gcctaccgga tcatctttgt ccatgtgccg gccgcctgga tggcgagctt cgcctatgcg
     6301 gcgatggcgg cggcgagctt cgtggccctc gtctggcgcc atccggtagc cgaggtctcg
     6361 gcacgggcga tagcgcccat cggggccgtc ttcaccgcca tagcgctctt caccggtgcc
     6421 gtctggggca agcccatgtg gggcacctgg tgggtctggg acgccaggct cacctcggtc
     6481 ctgatcctct tcttcctcta tctcggctac atggcgctgc acgacgcctt cgaggacccg
     6541 gcgcgcggtg ccagggcggc ctccatcctg gcgctggtcg gtgcggtcaa cctgccgatc
     6601 atccgcttct cggtcgactg gtggagcacg ctgcaccagc cggccaccat cctgcgcgcg
     6661 ggcgggccgg ccatggaccc gtccatgctc tacccgctgc tgctgatgat cctggtcgcc
     6721 aagctctaca tgatcgccgt gatgatcctg cgcatccgcc tgcagctcac cgagcgcaag
     6781 atccaaaacc tcaggctcgc cgccgagggg cggggctgag ccgcccggtc gcgctcagtc
     6841 gacgtcgaga tcgaagccga gccggccgat
//
LOCUS       DAOTFI010000246         6848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13174_length_6848_cov_7.469748, whole genome shotgun sequence.
ACCESSION   DAOTFI010000246 DAOTFI010000000
VERSION     DAOTFI010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13174_length_6848_cov_7.469748"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="P5558_19895"
     CDS             <1..1449
                     /locus_tag="P5558_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26641.1"
                     /translation="PPRYMANGEPAKVEKVDDTTVTFTFAAPYGDFLAELASPLGQHP
                     VLYAKHYCSQFLPQYNDQIDELIAANNASDWQNLYLAKCGDIEIPARWGNAERPTLDP
                     WVAVEPYTGGAVRVVMGRNPYFWQVDPEGNQLPYIDELVSPIAQDVESLILDAIGGRI
                     DFQIRHLDAAANRPVLAENREAGGYEFVEASPPGGVNMIINLNLTHKDPELRELFNKK
                     DFRVALSLGMDRQAIIDTALLGDGQPWQQGPFEDHPNYHEKISTQYLDFDQAEANRLL
                     DGIGLDKRGPDGMRLLPSGRPLKFQVDVIPTLQPEQVDMLELIEQYWAEIGVDMDVNA
                     LERTFFYERTSNSNDHDAAVWGGQASWVPGEIPQQLVPVHHDSRWGIPWSRWYNTGGA
                     EGEEPPASVKERMKLYDQARATVDPEKRRAIIHQIADIAAEEFEVMGVSKAVPTYGVT
                     KANLMNVPESMPSSWYYPTPAPTMLTTWYWAK"
     gene            1517..2500
                     /locus_tag="P5558_19900"
     CDS             1517..2500
                     /locus_tag="P5558_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26642.1"
                     /translation="MLTYIIKRILWTIPFLFAVSIVAFALIQAPPGDYLTTFAATLAQ
                     SGDMVDQERLEALRTRYGLDQPFIVQYWKWISGVLVGDFGISFEWQQPVSNLIWERMA
                     LSLCLAVSTLLFTWAVAFPIGIYSAVRKYSIGDYVVTTIGFLGLAMPNFLLALVLMYI
                     GVVYFGADVGGLFSPEYQTAPWSWAKVVDLGKHIWIPVVILGTSSSAALIRIMRANLL
                     DELNKPYVTTARAKGLSEFRLLLKYPVRLALNPFVSTIGWAFPQLISGAVITAFVLSL
                     PTAGPLLLQALLAQDMYLAGAFILLLCTLTVVGMLVSDILLALLDPRIRYR"
     gene            2505..3635
                     /locus_tag="P5558_19905"
     CDS             2505..3635
                     /locus_tag="P5558_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26643.1"
                     /translation="MASVLDPRLHTDTEVASQWRLMWWSFRRHTLAMVGLVIVLLLYL
                     IGFFAEFVAPFDPQSASAKHVYHPPQMIHFIDSTEEGWRIKPWVQAYTFERDKFTLQT
                     TFKPDEGKRIYLSFLGKGTPYELLGLFPAERRLLVPDEPNERFFLFGADRLGRDMLSR
                     TVFGTRVSMSIGLVGVTLSLLLGVLLGGISGYYGGAVDMVIQRVVEFVLSLPTIPIWL
                     ALTASLPATWPPLTRYFAITLILSLVGWTELARVVRGRFLALRSEDFVVAARLDGCSE
                     KRVIFRHMLPSLMSHVIASVTLAIPVMILAETSLSFLGLGLLPPTISWGVLLKEAQNI
                     RSIAQAPWLFVPGAFVLVAVLALNFLGDGLRDAADPYAQERG"
     gene            3635..5386
                     /locus_tag="P5558_19910"
     CDS             3635..5386
                     /locus_tag="P5558_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26644.1"
                     /translation="MEPVAAAEPGPAVLEVKGLVTEFPTRHGPFRAVDDVSFELRAGQ
                     TLCVVGESGSGKSVTARSILQIVDTPGRITAGEVLLHRHRTSRGSGEEETIDLARLEP
                     RGQAIRAIRGRDIAMIFQEPMSSLSPVHTIGNQIGEVIRLHESVSKKEARARSIELLR
                     QVEIPTPESAVDRYTFEFSGGMRQRAMIAMSLACNPSVLIADEPTTALDVTTQAEILD
                     LIKRLQVDHGMAVMLITHDMGVVAETADSIVVMRHGKLVERGSVEAIFYAPQHDYTKM
                     LLQAVLDLDRPSERRLARRAVQAIGGPVVEIKGLVKEFDLRHGFMRRKGHVLKALDDI
                     DLTLNEGENLGVVGESGSGKTTLGRCLLRVHDPSRGEMLYRAVGGGVADVATARGNGL
                     ERIRRDIRMIFQDPFASLNPRMNVAQIIGEPLLVNGVAKGAALRARVAELLDVVGLPA
                     SAMERYPHAFSGGQRQRIGIARAIALEPRVIVADEATSALDVSLRTQVLDLLLELQER
                     LGLSFIFISHDISVIRYFCDRVAVMYRGKVVEVGETEEVCTNPQHGYTKALLSAVPHP
                     DPRHRGMLGRHRYEAGR"
     gene            5383..6321
                     /locus_tag="P5558_19915"
     CDS             5383..6321
                     /locus_tag="P5558_19915"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY26645.1"
                     /translation="MRERLDPDMAEAMAFEAARDAGLPPPVDLDEERARGRDAAAFWN
                     EGLPEMAAIEDHVVAGPEGPVPVRVFKPVDSGPLPVMLYIHGGGWVVGSVVQNEPLIR
                     TLAARSGWAIAAPTYRLAPEHPYPAGLDDNIAAGQWLQNEGAAHGLDASRMVVSGTSA
                     GGNLALAAAVSGRLEALAGLVLFYGVFGADLDTASYRRFADGRFGLSRERMAYFLDLY
                     DPTGRHRTDPLLQPLLAELAGLPPTWLLAAGLDVLRSDTLALHSRLIEAGVRSTLHVE
                     PGVVHGFINRGRMVGAARRSLDGAAHFLRSLTRDTP"
     gene            6318..>6848
                     /locus_tag="P5558_19920"
     CDS             6318..>6848
                     /locus_tag="P5558_19920"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26646.1"
                     /translation="MKITAIETHLMQVGARPVSGTSRGSDGHGGSRNWLFVRVLTDEG
                     IYGVGEGSGWPRVVKAAVEDLARVLVGEDPAHIDRLWQRMYVASMGHGVLGTVGGGAM
                     TAIEMALWDIKGKALGQPVWNLLGGRFRDRVKVYGHAKTPERARELMGLGIKGVKVGF
                     TGAVDIDRVAAIRDAVG"
BASE COUNT         1022 a         2515 c         2192 g         1119 t
ORIGIN      
        1 ccgccgcgct acatggcgaa tggcgagccg gccaaggtcg agaaggtcga cgacacgacg
       61 gtgaccttca ccttcgccgc accttacggc gatttcctcg ccgagctggc ctcgcccttg
      121 ggccagcatc cggtgctcta cgccaagcac tactgctcgc agttcctgcc gcagtacaac
      181 gaccagatcg acgagctgat cgccgccaac aacgccagcg actggcagaa cctctatctc
      241 gccaagtgcg gcgacatcga gatccccgcc cgctggggca atgccgagcg cccgacgctc
      301 gacccgtggg tggccgtcga gccctatacc ggcggtgcgg tgcgcgtcgt catgggtcgc
      361 aacccgtatt tctggcaggt cgaccccgag ggcaaccagc tcccctatat cgacgagctg
      421 gtctcgccga tcgcccagga cgtcgagagc cttatcctcg acgcgatcgg cgggcggatc
      481 gacttccaga tccgccatct cgatgccgcg gccaaccgcc cggtgctcgc cgagaaccgc
      541 gaggccggcg gctacgagtt cgtcgaggcg agcccgcccg gcggcgtcaa catgatcatc
      601 aacctcaacc tgacccacaa ggacccggag ctgcgcgagc tcttcaacaa gaaggacttc
      661 cgcgtcgcgc tctccctcgg catggaccgg caggcgatca tcgacacggc cttgctcggt
      721 gacggccagc cctggcagca ggggccgttc gaggatcatc cgaactacca cgagaagatc
      781 agcacccagt atctcgactt cgaccaggcg gaagccaatc gcctgctcga cggcatcggc
      841 ctggacaagc gcggcccgga cggcatgcgg ctcttgccga gcggcaggcc cttgaagttc
      901 caggtcgacg tcatcccgac cctgcagccc gagcaggtcg acatgctcga gctgatcgag
      961 cagtactggg ccgagatcgg cgtcgacatg gacgtcaacg ccctcgagcg gacgttcttc
     1021 tacgagcgca cgtcgaacag caacgaccac gacgcggcgg tctggggcgg ccaggcgagc
     1081 tgggtcccgg gggagatccc gcagcagctc gtgccggttc accacgacag ccgctggggc
     1141 atcccctgga gccgctggta caacacgggc ggcgccgagg gcgaggagcc gccggcctcg
     1201 gtcaaagagc gcatgaagct ctacgaccag gcgcgcgcca cggtcgatcc ggagaagcgc
     1261 cgggcgatca tccaccagat cgccgatatc gccgccgagg agttcgaggt gatgggcgtg
     1321 agcaaggccg tgccgaccta cggcgtcacc aaggccaacc tcatgaacgt tcccgagtcg
     1381 atgccgagct cctggtacta tccgaccccg gcgccgacca tgctgacgac ctggtactgg
     1441 gcgaaatgag ctcgcggtcg cgccggcacc acgccggcgc ggcccgcctt cttgaccaga
     1501 ggacagggca gccatgctgc tgacctacat catcaagcgg atcctctgga ccattccttt
     1561 cctcttcgcc gtgtcgatcg tcgcgttcgc gctgatccag gcaccgcccg gcgactacct
     1621 cacgaccttc gccgccacgc tggcccagtc gggcgacatg gtggaccagg agcggctcga
     1681 ggcgctccgg acccgctacg ggctcgatca gcccttcatc gtccagtact ggaaatggat
     1741 ctcgggcgtg ctggtcggcg atttcggcat ctccttcgag tggcagcagc cggtatccaa
     1801 cctgatctgg gagcgcatgg cgctctcgct ctgcctcgcc gtctcgaccc tgctcttcac
     1861 ctgggcggtc gcctttccga tcggcatcta ctcggccgtg aggaaatact cgatcggcga
     1921 ctacgtcgtc acgacgatcg gctttctcgg cctcgccatg cccaatttcc tcctggcgct
     1981 ggtgctcatg tatatcggcg tcgtctattt cggcgccgat gtcggcgggc tcttctcgcc
     2041 cgagtaccag accgccccct ggtcctgggc caaggtcgtc gacctcggca agcacatctg
     2101 gatcccggtg gtcatcctcg gcacctcgtc ctcggcggcg ctgatccgga tcatgcgcgc
     2161 caacctgctc gacgagctca acaagcccta cgtgaccacg gcccgcgcca aggggctctc
     2221 cgagttccgt ctgctcctga agtacccggt gcggctcgcc ctcaacccct tcgtcagcac
     2281 gatcggctgg gccttcccgc agctcatctc gggtgcggtc atcaccgcct tcgtcctgtc
     2341 cctgcccacc gccgggccgc tgctgctgca ggcgctgctg gcgcaggaca tgtatctcgc
     2401 cggcgcgttc atcctgctgc tctgcacgct gacggtcgtc ggcatgctgg tctcggacat
     2461 cctcctggcg ctgctcgacc cacgcatccg gtatcgctga gcgcatggcc tccgtcctcg
     2521 atccccgcct gcacaccgat accgaggtcg cctcgcaatg gcggctgatg tggtggtcgt
     2581 tccgccggca cacgctcgcc atggtcggcc tcgtgatcgt gctgctgctc tacctgatcg
     2641 gcttcttcgc cgagttcgtc gcccccttcg acccgcagtc ggcgagtgcg aagcacgtct
     2701 accacccgcc acagatgatc cacttcatcg acagcacgga ggagggctgg cggatcaagc
     2761 cctgggtcca ggcctacacg ttcgagcgcg acaagttcac cctgcagacg accttcaagc
     2821 cggacgaggg caagcggatc tatctctcgt tcctcggcaa gggcacgccc tacgagctcc
     2881 tcgggctctt cccggccgag cggcgccttc tcgtgccgga cgagccgaac gagcgcttct
     2941 ttctcttcgg cgccgatcgc ctcggccgcg acatgctgag ccggacggtc ttcggcaccc
     3001 gcgtctcgat gtccatcggc ctcgtcggcg tgacgctcag cctgctgctc ggcgtgctgc
     3061 tcggcggcat ctccggctac tatggcgggg ccgtcgacat ggtgatccag cgcgtcgtcg
     3121 agttcgtgct gtccctgccg acgatcccga tctggctggc gctcaccgcc tcgctgccgg
     3181 cgacctggcc gccgctcacc cgctatttcg cgatcaccct gatcctctcg ctggtcggct
     3241 ggaccgagct ggcaagggtg gtgcgcggcc gcttcctcgc cttgcgcagc gaggatttcg
     3301 tcgtcgccgc caggctcgac gggtgcagcg agaagcgggt gatcttccgc cacatgctgc
     3361 catccctgat gagccacgtg atcgcctcgg tcacgctcgc cattcccgtc atgatcctgg
     3421 ccgagacctc gctctcgttt ctcggcctcg gcctcctgcc gccgacgatc tcatggggcg
     3481 tcctcctcaa ggaggcgcag aacatccgct cgatcgccca ggcgccctgg cttttcgtcc
     3541 ccggcgcctt cgtcctggtc gccgtcctgg cgctcaattt cctcggcgac gggctgcgcg
     3601 atgccgccga cccctatgcc caggagcgtg gctgatggag cccgtcgccg cggcggagcc
     3661 ggggccggcg gtgctggagg tcaaggggct cgtcaccgag ttcccgaccc gccacggccc
     3721 ctttcgcgcg gtcgacgacg tcagcttcga gctccgggcc ggccagacgc tctgcgtggt
     3781 cggcgagagc ggcagcggca agagcgtcac ggcacgctcg atcctgcaga tcgtcgacac
     3841 gcccggccgc atcaccgccg gcgaggtgct gctccaccgg catcgcacct cgcgcggctc
     3901 gggcgaggag gagacgatcg acctggcgcg gctggagccc cgcggccagg cgatcagggc
     3961 gatccgcggc cgcgacatcg cgatgatctt ccaggagccg atgagctcgc tctcgcccgt
     4021 ccacaccatc ggcaaccaga tcggcgaggt gatccggctg cacgagagcg tctcgaagaa
     4081 ggaggcgcgc gcccggtcga tcgagctgct ccgccaggtc gagatcccga cgccggagag
     4141 tgccgtcgac cgctacacct tcgagttctc gggcggcatg cggcagcggg cgatgatcgc
     4201 catgtcgctc gcctgcaatc cctcggtgct gatcgccgac gagcccacca ccgccctcga
     4261 cgtcaccacc caggccgaga tcctcgacct catcaagcgg ctgcaggtcg atcacggcat
     4321 ggccgtgatg ctgatcaccc acgacatggg cgtggtcgcc gagacggccg actcgatcgt
     4381 cgtcatgcgc cacggcaagc tggtcgagcg cggctcggtc gaggcgatct tctacgcgcc
     4441 gcagcacgac tacacgaaga tgctgctgca ggcggtgctc gacctcgacc gcccgtccga
     4501 gcggcgcctc gcccgccgtg ccgtccaggc gatcggcggc ccggtcgtcg agatcaaggg
     4561 gctcgtcaag gagttcgacc tccgccacgg cttcatgcgg cgcaagggcc atgtgctgaa
     4621 ggcgctcgac gacatcgacc tgaccctcaa cgagggcgag aatctgggcg tcgtcggcga
     4681 gagcggcagc ggcaagacca ccctcggccg ctgcctgctc cgggtccacg acccgagccg
     4741 cggcgagatg ctctaccggg ccgtcggtgg cggcgtcgcc gatgtcgcca ctgccagggg
     4801 caacggcctc gagcgcatcc gccgcgacat ccgcatgatc ttccaggacc cgttcgcctc
     4861 cctgaacccg cgcatgaacg tggcgcagat catcggcgag ccgctgctcg tcaacggagt
     4921 cgccaaaggc gccgccctgc gcgcccgcgt cgccgagctt ctcgacgtcg tcggcctgcc
     4981 ggcctcggcc atggagcgct acccgcacgc cttctcgggc gggcagcgcc agcgcatcgg
     5041 catcgcccgc gccatcgcgc tggagccccg ggtcatcgtc gccgacgagg cgacctcggc
     5101 cctcgacgtg tcgctgcgca cccaggtcct cgatctcctg ctcgaattgc aggagcggct
     5161 cggcctctcc ttcatcttca tcagccacga catcagcgtg atccgctatt tctgcgaccg
     5221 ggtggcggtc atgtatcggg gcaaggtggt cgaggtcggc gagaccgagg aggtctgcac
     5281 caacccgcag cacggctaca ccaaggcgct cctgtccgcc gtcccgcatc ccgacccgcg
     5341 ccatcggggc atgctcggcc gccatcgcta cgaggcaggc agatgagaga acggctcgac
     5401 cccgacatgg ccgaggcgat ggccttcgag gccgcccgcg acgcaggtct gccgccgccg
     5461 gtcgacctcg acgaggagcg ggcacgcggc cgggacgcgg ccgccttctg gaacgagggc
     5521 ctgcccgaga tggcggcgat cgaggaccat gtcgtcgccg gccccgaggg ccctgtgccc
     5581 gtgcgcgtgt tcaagccggt cgacagcggg ccgctgcccg tcatgctcta catccatggc
     5641 ggtggctggg tggtcggcag cgtcgtgcag aacgagcccc tgatccgcac gctcgccgcc
     5701 cgctccggct gggcgatcgc ggcacccacc tatcgcctcg cccccgagca tccttaccct
     5761 gccggcctcg acgacaacat cgccgccggc cagtggctgc agaacgaagg cgccgcccac
     5821 gggctggacg cgagccggat ggtcgtctca ggcacctcgg ccggcggcaa cctggcgctc
     5881 gccgcggcgg tctcgggaag gctcgaggcg ctcgccggcc tcgtcctctt ctacggcgtc
     5941 ttcggcgccg atctcgacac cgccagctat cgccgcttcg ccgacggccg cttcggcctc
     6001 tcgcgcgagc gcatggcata tttcctcgat ctctacgatc cgaccggccg gcaccgcacc
     6061 gatcccctgc tccagcccct gctcgccgag cttgccggcc tgccaccgac ctggctgctc
     6121 gccgccggcc tcgacgtgct ccgctccgac accctggcgc tgcacagccg cctgatcgag
     6181 gcgggcgtgc gctcgaccct ccacgtcgag ccgggcgtcg tgcacggctt catcaatcgt
     6241 ggccggatgg tcggggcggc cagacggtcg ctcgacggcg ccgcccattt ccttcgctcg
     6301 ctcacacggg ataccccatg aagatcaccg ccatcgaaac ccacctcatg caggtcggcg
     6361 cccgtccggt cagtggcacg agccggggct cggacggcca cggcggctcg cgcaactggc
     6421 tgttcgtccg ggtgctgacc gacgagggga tttacggtgt cggcgagggc tcgggctggc
     6481 cgcgggtggt caaggccgcc gtcgaggact tggcccgtgt gctggtcggc gaggaccctg
     6541 cccatatcga ccggctctgg cagcgcatgt acgtggcctc gatgggccac ggcgttctcg
     6601 gcacggtcgg cggcggcgcc atgaccgcga tcgagatggc gctctgggac atcaagggca
     6661 aggcgctggg ccagcccgtc tggaacctgc tcggcggccg gtttcgcgac cgcgtcaagg
     6721 tctacggcca cgccaagacg ccggagcgcg cacgcgagct gatggggctc ggcatcaagg
     6781 gcgtgaaggt cggcttcacc ggggccgtcg acatcgaccg ggtcgccgcc atccgcgacg
     6841 ccgtgggg
//
LOCUS       DAOTFI010000247         6754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13429_length_6754_cov_5.931781, whole genome shotgun sequence.
ACCESSION   DAOTFI010000247 DAOTFI010000000
VERSION     DAOTFI010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13429_length_6754_cov_5.931781"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="P5558_19925"
     CDS             <1..1164
                     /locus_tag="P5558_19925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY26647.1"
                     /translation="GTGAYDALNVIPSWMADLVRAGALEPLDPYVDKYGYRDELQTIA
                     PVYRDNQMLVGDTIYGFPDDGDVFVFYYRTDVLGDPEIQAAFKEAKGYDLPVPPTDWQ
                     QFYDVSCFITEHTGGKPYGAGLFRDPPYAQQMFQERFRVAGGKFFDPETMQATVNSEA
                     GVGVFQSMLEENKCMPPGVETWGFVENLAAFLQGDTAMTISWPPYGRWAAGYGLDQEA
                     FSWVPKSQIAGKVGYALTPGGHPQLASGFSLAVSSDSNNKEAAYLFIQWLNSEEVSID
                     RVQLPYALRDPFRDSHFTSEEYKSRWPEAPQYLEALQAGAVSGILDLSLLQTDRYEEA
                     LRQGISRLWAGEDPQAILDDVAAQWDAITERVGVDAQREAYLDWSSKPNAYPN"
     gene            1222..2109
                     /locus_tag="P5558_19930"
     CDS             1222..2109
                     /locus_tag="P5558_19930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY26648.1"
                     /translation="MQPASGQAFVGRGFKYGMVWPAVLVILLIGLFPIVYTAIVSFQN
                     IDMFGEDTSFQGGVHYARLMGDARFWQSVLHTMIFLVVALPLELVLGLLLALLFLDRL
                     PGKQIFVALLILPVVTSPIIAGSTWRLLFDNRYGPINQILSWLTGEPATVLWTVNPVY
                     VYPAILLAEVWQWTPFMFLLLLAGLSNVDQSLNEAAELDGAGFWKTFWYVTLPIIKPV
                     ILIACLIRALDLFRLFDVVWALTRGGPGTMTETVSIYAYVLAFKQFETSYAAALAVVI
                     IVLLSLVVMAALRRVEFAR"
     gene            2106..2927
                     /locus_tag="P5558_19935"
     CDS             2106..2927
                     /locus_tag="P5558_19935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY26649.1"
                     /translation="MRQSPRTLALRYLLVALALVFFLFPIYWLVAISLKTPDEIFASP
                     PIWVPGQIQFQNYAVLFKDGDVWTIWNSLVVATLSTAVAMLLGTMCAYSMARFRTGGD
                     HFAMWVISNRMIPPIVIVFPVFLLYVKLGWVDTRLGLIVLYTAFNLPYVIWMMRGYIQ
                     DVPIELEESALVDGCSRLQTFRLIVLPVIRGGLFATAVFTFVFAWNEFVFALVLTRSN
                     ATTFPVQVTHYFGGQSNFWAKIAAMSTLGTIPIFIAVAMLQKFLVRGVSMGAVKG"
     gene            3052..3891
                     /locus_tag="P5558_19940"
     CDS             3052..3891
                     /locus_tag="P5558_19940"
                     /inference="COORDINATES: protein motif:HMM:NF018436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HRY26650.1"
                     /translation="MERNHVKVAARIGYAARGAVYLLLGGLAFLAAIGSGGQTTDPKG
                     ALAALLGEPLGFVIVLAIGIGLFAYAAWRAVQGLLDADRHGTDAKGLAIRGAMCVSAV
                     THASLGLYAVTLPFALGAFSNGGGDGSKDAASWVLRQPFGRYLLALVALAILAAGLAQ
                     IHKALTGGFRERLAMREALLDRLSPVCAFGLAARGVVFLIIGGFFAYAAWVIDPDEAG
                     GTAAALEWLRSQPYGSWLFLAIAIGLVAFGMYGLIQAIWRRIDLDEPARHADRLTALG
                     NRS"
     gene            complement(3898..4623)
                     /locus_tag="P5558_19945"
     CDS             complement(3898..4623)
                     /locus_tag="P5558_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY26651.1"
                     /translation="MHTGHNLADRVAVVTGGSRGIGRAIVAALRRAGAIAVVWDRDLP
                     AADATDGIAVDVTDRVALEGAVATVRERHGRIDILVNNAGYAGSTVPVVDYPEDEWRR
                     ILEVNLTGTFLVCRTVVPTLVANGWGRIVNVASLAGKEGTPNASAYSASKAGVMALTK
                     SLGKELATTGVLVNAVAPAAVRTSLLDQMAPAHVQTMIVKSPMQRLGEPEEVAELVLW
                     LCSPACSFSTGAVFDLSGGRAVY"
     gene            4745..5857
                     /locus_tag="P5558_19950"
     CDS             4745..5857
                     /locus_tag="P5558_19950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY26652.1"
                     /translation="MPPPASPKDKRMVGDVSTPEEGILASRALQVAIRVGLVLLLIAW
                     CFTIIRPFLVPVVWGIVIAVATYNLFAALQRLLGGRAGLAAFAYATIALLVLILPALL
                     LTGTHVDAVRELANGLLGGTLRLPPPPPAIETWPLIGEPLTSFWQLASSNLEAAIREI
                     GPELAVAGRWLLGLVGATALGLIQFVLAIVIAAALLSNAASGGQLAGDIATRLAGARG
                     PTYATLAVQTIRTVARGIIGVALIQSTLAGLGFLAVGQPMAGFLAMLCLVLAILQVGP
                     LPVILPVVVYQFTVLDTTTAALFTAWCVLVSLIDNVLKPILLGRGVDLPMLVIFIGAI
                     GGVMSSGLLGLFIGPVVLALGYTLFMAWLKDARATA"
     gene            6071..>6754
                     /locus_tag="P5558_19955"
     CDS             6071..>6754
                     /locus_tag="P5558_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY26653.1"
                     /translation="MLSRLIDSPRRRAACAAAILLATTAIVPFASNWALPVNAATPPS
                     TTGAQLPGSFADLVESVSPAVVSIVTTQPAPEQMAGMPELPPDSPFAPFFRDFGGPSA
                     PKHQAPRHDATSLGSGFIIDAEGYVVTNNHVIADATEIEVVLADGTRLDATLVGRDDK
                     VDLALLKVESDEALPYLTFGDSDKLRVGDWTIAVGNPFGLGGTVTAGIVSARGRDIHS
                     GPYDDYLQLD"
BASE COUNT          953 a         2590 c         2035 g         1176 t
ORIGIN      
        1 ggaaccggcg cctatgacgc gctcaacgtc atcccctcct ggatggccga tctcgtgcgc
       61 gccggcgccc tcgagccgct cgacccctat gtcgacaaat acggctaccg cgacgagctg
      121 cagacgatcg cccccgtcta tcgcgacaac cagatgctgg tcggcgacac catctacggc
      181 ttccccgacg acggtgacgt cttcgtcttc tactaccgca ccgacgtgct cggcgatccc
      241 gagatccagg ccgccttcaa ggaggccaag ggctacgacc tgccggtgcc gccgaccgac
      301 tggcagcagt tctacgacgt cagctgcttc atcaccgagc ataccggcgg caagccctac
      361 ggcgccggcc tcttccgcga cccgccctac gcgcagcaga tgttccagga acgcttccgc
      421 gtcgccggcg gcaaattctt cgatccggag accatgcagg ccacggtcaa cagcgaggcc
      481 ggtgtcggcg tcttccagag catgctggaa gagaacaagt gcatgccgcc cggcgtcgag
      541 acctggggct tcgtcgagaa cctcgcggcc ttcctccagg gcgacacggc gatgaccatc
      601 tcctggccgc cctatggccg ctgggccgcc ggctacggcc tcgaccagga ggccttctcc
      661 tgggtcccca agtcgcagat cgccggcaag gtcggctacg ccctcacgcc gggcggccac
      721 ccgcagctcg cctcaggctt ctcgctcgcc gtctcctccg actcgaacaa caaggaggcg
      781 gcctacctct tcatccagtg gctcaattcc gaggaggtca gcatcgaccg cgtgcagttg
      841 ccctacgcgc tgcgcgaccc gttccgcgac agccacttca ccagcgagga gtacaagtcg
      901 cgctggcccg aggcgccgca atacctcgag gccctgcagg ccggggccgt gagcggcatc
      961 ctcgacctct cgctgctgca gaccgaccgc tacgaggagg cgcttcggca gggcatctcg
     1021 cggctctggg ccggcgagga tccgcaggcc attctcgacg acgtcgccgc ccagtgggat
     1081 gcgatcaccg agcgcgtcgg cgtcgacgcc cagcgcgagg cctatctcga ctggtcgtcc
     1141 aagcccaacg cctacccgaa ctgagcgttc gagcgggtgg gggcggcccg gccgccccca
     1201 ccccgtccca gccctgacca catgcaacca gcttcaggac aggccttcgt cggccgcggc
     1261 ttcaaatatg gcatggtctg gccggccgtg ctggtcatcc tgctgatcgg cctcttcccg
     1321 atcgtctaca cggccatcgt cagcttccag aacatcgaca tgttcggcga ggacacgagc
     1381 ttccagggcg gcgtgcatta cgcccgcctg atgggcgacg cccgcttctg gcagtcggtc
     1441 ctccacacca tgatcttcct cgtggtggcc ctgccgctcg agctcgtgct cggcctcctt
     1501 ctcgccctcc tcttcctcga ccgcctgccc ggcaagcaga tcttcgtggc cctcctgatc
     1561 ctgcccgtgg tcacctcgcc gatcatcgcc ggctccacct ggcgactgct cttcgacaac
     1621 cgctacggcc ccatcaacca gatcctctcc tggctcacgg gcgagccggc caccgtgctc
     1681 tggaccgtca accccgtcta cgtctacccg gccatcctcc tcgccgaggt ctggcagtgg
     1741 acgcccttca tgttcctcct gctgctcgcc gggctctcga atgtcgacca gtcgctcaac
     1801 gaggcggccg agctcgacgg tgccggtttc tggaagacct tctggtacgt caccctgccg
     1861 atcatcaagc cggtgatcct gatcgcctgc ctgatccgcg ccctcgatct cttccggctc
     1921 ttcgacgtcg tctgggcgct gacccgcggc ggccccggca cgatgaccga gaccgtctcg
     1981 atctacgcct acgtgctcgc cttcaagcag ttcgagacga gctacgccgc ggccctcgcg
     2041 gtcgtcatca tcgtcctgct ctcgctcgtc gtcatggcgg cgctgcgccg ggtcgagttc
     2101 gcccgatgag gcagtcgccc cgcaccctgg cgctccgcta cctgctggtg gcgctggccc
     2161 tcgtcttctt cctcttcccg atctactggc tcgtggcaat ctcgctcaag acgccggacg
     2221 agatcttcgc gagcccgccg atctgggtgc cgggccagat ccagttccag aactatgccg
     2281 tgctgttcaa ggacggcgac gtctggacca tctggaacag cctcgtcgtc gccacgctca
     2341 gcaccgccgt cgccatgctg ctcggcacca tgtgcgccta ctccatggcg cgctttcgga
     2401 caggcggcga ccatttcgcc atgtgggtca tctcgaaccg gatgatcccg cccatcgtca
     2461 tcgtcttccc cgtcttcctc ctctacgtga agctcggctg ggtcgacacg cgcctcggcc
     2521 tcatcgtcct ctacaccgcc ttcaacctcc cctacgtgat ctggatgatg cgcggctaca
     2581 tccaggacgt gccgatcgag ctcgaggaat cggcgctcgt cgacggctgc tcgcggctgc
     2641 agaccttccg cctgatcgtc ctgccggtga tccggggcgg gctcttcgcc accgccgtct
     2701 tcaccttcgt cttcgcctgg aacgagttcg tcttcgccct cgtgctgacc cgcagcaacg
     2761 ccaccacctt ccccgtccag gtcacccact atttcggcgg ccagtccaac ttctgggcca
     2821 agatcgccgc catgagcacg ctcggcacca tcccgatctt catcgccgtg gccatgctgc
     2881 agaagttcct ggtgcgcggc gtctcgatgg gggcggtcaa gggctagccg ggcgcttctc
     2941 gaccgcgctg acaagcaccc tgcaaccttt gcccaccccg cgagtttctt cccatcgagc
     3001 cccccgacgg cacgcttcca caggcacgcg agccaccagc ccggaggtcg gatggaaagg
     3061 aaccacgtca aggtcgcggc ccggatcggc tacgcggcgc gcggcgccgt ctacctgctt
     3121 ctcggcggcc tcgccttcct cgccgcgatc ggcagcggcg gacagaccac cgaccccaag
     3181 ggtgccctgg ctgccctgct gggcgagccg ctcggcttcg tgatcgttct cgccatcggc
     3241 atcggcctct tcgcctacgc ggcgtggcgg gcggtgcagg ggctgctcga cgccgaccgg
     3301 cacggcaccg atgcgaaggg gctggcgatc cgtggcgcca tgtgcgtcag cgccgtcacc
     3361 catgcctcgc tcgggctcta cgccgtcacc ctgcccttcg cgctcggcgc cttctcgaac
     3421 ggcggcggcg acgggtccaa ggacgcggcg tcatgggtgc tgcgccagcc cttcggccgc
     3481 tatctcctgg cgctggtcgc cctcgccatc ctcgccgccg gcttggcgca gatccacaag
     3541 gccctcaccg gcggctttcg cgagcggctg gccatgcgcg aggcgctgct cgaccggctt
     3601 tcgccggtct gcgccttcgg cctcgccgcc cgcggcgtcg tcttcctgat catcggcggc
     3661 ttcttcgcct acgctgcctg ggtcatcgac ccggacgagg ccggcggcac ggccgcagca
     3721 ctcgaatggc tgcgctcgca gccctatggc agctggctct ttctcgccat cgcgatcggg
     3781 ctcgtcgcct tcggcatgta cggcctcatc caggccatct ggcgccgcat cgacctcgac
     3841 gagccggccc gacacgccga ccggctgacc gccctcggca accgctcgtg acctgcccta
     3901 atagaccgcc cggccgcccg aaaggtcgaa gacggcaccc gtgctgaagc tgcaggccgg
     3961 cgagcagagc cagagcacga gctcggcgac ctcctcgggc tcgccgagcc gctgcatcgg
     4021 gctcttcacg atcatcgttt gcacgtgcgc cggcgccatc tggtcgagga gcgacgtccg
     4081 aaccgccgcc ggggcgaccg cattgaccag cacgcccgtg gtggcgagct ccttgccgag
     4141 cgacttggtc agcgccatga cgccggcctt cgacgcgctg taggcgctgg cattgggtgt
     4201 gccctccttg ccggcgagcg acgcgacatt gacgatccgc ccccagccgt tcgcgacgag
     4261 cgtcggcacg accgtcctgc aaaccagaaa cgtgccggtg agattgacct ccaggatccg
     4321 ccgccactcg tcctccggat agtcgaccac cggcacggtc gagccggcat agccggcatt
     4381 gttgacgagg atgtcgatcc ggccatgcct ctcccggacc gttgccacgg ccccctcgag
     4441 cgccacccgg tcggtgacgt cgacggcgat gccgtcggtc gcgtcggccg ccggcaggtc
     4501 acggtcccag accaccgcga tcgcgccggc ccgccgcagc gccgccacga tggcgcggcc
     4561 gatcccgcgc gagccgcccg tgacgacggc cacccgatcc gccagattgt gccccgtgtg
     4621 catgcctgct ccttgtccag ccgcgccagg ccgaccagtt gccgcccgtc ggcgacgccg
     4681 tcaaacgact tgcgcgacca gcctggccga agaccgcttg ccgcgacctg cccggcgatc
     4741 gagaatgccg ccgcccgcga gcccgaagga caagcgcatg gtcggcgacg tctcgactcc
     4801 cgaggaaggc atcctggcca gccgggcgct gcaggtcgcg atccgcgtcg gcctcgtcct
     4861 gctgctgatc gcctggtgct tcaccattat ccggccgttc ctcgtgcccg tggtctgggg
     4921 catcgtcatc gcggtcgcca cctacaacct ctttgcggca ctgcagcgcc tgctcggcgg
     4981 ccgggccggc ctcgccgcct tcgcctacgc gaccatcgcg ctgctcgtcc tgatcctgcc
     5041 ggccctgctg ctgaccggca cccatgtcga cgcggtgcgc gagctcgcca acggcctcct
     5101 gggcggcacg ctgcgcctcc cgccgccccc tcccgccatc gagacctggc cactgatcgg
     5161 tgagccgctc accagcttct ggcagctcgc ctcgagcaat ctcgaggcgg cgatcaggga
     5221 gatcggcccg gagctcgccg ttgccggtcg ctggctgctg ggcctcgtcg gggcgacggc
     5281 gctcggcctc atccagttcg tcctggccat cgtcatcgcc gcggctctgc tcagcaatgc
     5341 cgccagcggc ggccagctcg ccggcgacat cgccacccgc ctcgccggtg cccggggacc
     5401 gacctacgcg accttggccg tgcagaccat ccgcaccgtg gcgcgtggca tcatcggcgt
     5461 ggccctgatc cagtccaccc tggcgggtct cggcttcctc gccgtcggcc agcccatggc
     5521 cggcttcctc gccatgctct gcctcgtcct cgcgatcctg caggtcggcc cgctgccggt
     5581 gatcctgccg gtggtcgtct accagttcac cgtgctcgac acgaccacgg ccgccctgtt
     5641 caccgcctgg tgcgtgctcg tctcgctgat cgacaatgtc ctcaagccga tcctgctcgg
     5701 gcgcggcgtc gacctgccga tgctcgtcat cttcatcggc gccatcggcg gcgtcatgtc
     5761 ctccggcctg ctcggccttt tcatcggacc ggtggtgctg gcgctcggct acacgctgtt
     5821 catggcgtgg ctgaaggatg cacgggcaac ggcgtgagac gaccaagcgg aaacgatcag
     5881 acaacacgaa agaagacagc gactccgcac ggtgggagtg gcgtcggcca ttgtgaccga
     5941 tttgtcattt aacagattta atcgcccgtc tatggaatgc ggtcggctga tctcttattc
     6001 aaggatgacc gctttgcccc ggcaccgccc cgggcggcgg cccgaggttc accagactgg
     6061 gatctgctcc atgctcagcc gtttgatcga ttcgccgagg cggcgtgccg cctgtgccgc
     6121 ggccatcctc ctcgccacca cggccatcgt gccgttcgcg tcgaactggg ccctgcccgt
     6181 caacgcagcg accccgccca gcaccacggg cgcccagttg cccggcagct tcgccgacct
     6241 ggtcgagagc gtctcgccgg ccgtggtcag catcgtcacc acccaacccg cccccgagca
     6301 gatggccggc atgccggagc tgccgccgga ttcgcccttc gcgccctttt tccgcgactt
     6361 tggcggtcca tcggcaccga agcaccaggc gccgcggcac gatgccacct cgctcggctc
     6421 gggcttcatc atcgatgccg aaggctatgt cgtcaccaac aaccacgtca tcgccgatgc
     6481 gaccgagatc gaggtggtcc tggccgacgg cacccgcctc gacgccaccc ttgtcggccg
     6541 cgacgacaag gtcgacctcg ccttgctcaa ggtcgagagc gacgaggcgc tgccgtacct
     6601 gaccttcggc gacagcgaca agctccgcgt cggcgactgg accatcgccg tcgggaaccc
     6661 cttcggcctg ggcggcacgg tgaccgccgg catcgtctcg gcgcggggcc gggacatcca
     6721 ttcaggtccc tacgacgact acctgcagct cgat
//
LOCUS       DAOTFI010000248         6724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13498_length_6724_cov_6.837007, whole genome shotgun sequence.
ACCESSION   DAOTFI010000248 DAOTFI010000000
VERSION     DAOTFI010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13498_length_6724_cov_6.837007"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..848
                     /locus_tag="P5558_19960"
     CDS             15..848
                     /locus_tag="P5558_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY26654.1"
                     /translation="MPAKDIAELLACRFGEVPELAPEVAGVPLAEALAGRASCRAFAP
                     RPVDVALLRSLAALALCAPSKSDLQQADIVLATDPELRAALAATVPGQAWVAGTPALL
                     VVCGNNRRQRQVSEMRGREFANDHLDAFFNAAVDGGIVLATLVLAAEAAGLGACPISG
                     LRDRPEEVSDLLGLPAHVFPLAGLALGYPVREGRISPRLPLEVTVHENRWSEAGTAGR
                     MAAYDARRRELQPYASQRGLDRFGEVSDYGWLEDKARQYAEPQRADFGAFVRRRGFRL
                     D"
     gene            complement(855..1679)
                     /locus_tag="P5558_19965"
     CDS             complement(855..1679)
                     /locus_tag="P5558_19965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26655.1"
                     /translation="MAGFRRRLEERGWHWLARTITGLVLVFLLAPILVVIPISLTAGS
                     QLVFPMPGWSIQWYLEVLNSQAWRDAAANSLFVGCFATLLALLVGVPAALGLAGTTFR
                     GKRLIMMLVLSPMIVPIVITAVAVYFFFVQIGLVGTYLGLILAHAMLGAPFVVITVAA
                     TLENFDPWLMRAAAGLGAPPLVAFRRVMLPLILPGVATGGLFAFATSFDEIVVALFLA
                     APDQRTLPRQIFSGVREYVSPAIAAVAVMLLVLSTTLLATVEVLRRRSARLRGTTP"
     gene            complement(1679..2905)
                     /locus_tag="P5558_19970"
     CDS             complement(1679..2905)
                     /locus_tag="P5558_19970"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26656.1"
                     /translation="MLRHDPHLTRRAFAAQRRRQLLRRAGLLLPLLLFVLVTFAGPIV
                     LFLWRAVDNAIVPETLPRTVAEIVHWDRTGLPPDPVFDALALDLRGAREGGRAALLGK
                     RLNAARVGYRSLVLKTANKLPEVPDGSWRETLIAIDGKWGEPGWWTSLRNESGTVTPS
                     NLLATVDLQLDSAGEVEAVPPEMALYRMLLGRTFVIALQVTALCLLLGYPTAAVLAVL
                     PQRTTNLLLIAVLLPFWTSLLVRTTAWVILLQGNGPVNGLLEALGLIAEPLQLIFNRG
                     GTLTAMVHVQLPFMILPLYAVMRTIPAEQMRAALSLGAAPRAAFLRVWLPQTLPGVGA
                     GALLVFIMSLGYYITPALVGGPGDQMLSWFVTYQLNEAGNWGMAAALGSLLLLATLLA
                     VAFLGRLLGATRLVGH"
     gene            2981..3673
                     /locus_tag="P5558_19975"
     CDS             2981..3673
                     /locus_tag="P5558_19975"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY26657.1"
                     /translation="MTTRQLPDESGPGPFPVQRLQRATLNDEVYHELRRAIMSGAIEP
                     GSTMTIRGLAQSFGISLMPVRQALNRLVAEHVLVLLPNRSVALPRIDAARFREITRIR
                     VALEGLAAEEAVAHVDADAIARMAAANVAIEAGAAPGEALALNREFHFTLYAASAMPT
                     LVAMIETTWLQVGPLLHVPIRRMMDGAAPRSLPAHRRVLAGLRAGDAAEVRDAIRADL
                     EDAAVDIAATLD"
     gene            complement(3731..4792)
                     /locus_tag="P5558_19980"
     CDS             complement(3731..4792)
                     /locus_tag="P5558_19980"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26658.1"
                     /translation="MQARLRSVVLGGVATFLAAGLMAGTAAAQEQVIVVGWGGVWQDA
                     YRKALFEPFAAETGIKVVEEEFGGEYAKLTAQIEAGKITWDLAAFESPQVIQGCDEGA
                     FVPLDWDALGGRDNQLDYAAHDCGIASDVWATVLAYDADKLNPGPTSWQDYWDTTKFP
                     GKRGAYKDARIMLEIALVADGVPGDQIYDVLATPEGVDRAFAKLDEIKPDLLWAESPA
                     DGMQRLLAGDVVMTINFNARVTGASRDNQRNLVIAWPAGFWVGTDYWVQIEGGPNPGP
                     AAKLIEFYSRPETQAELVKHLSYGVPSREAYALMSDEVKAELPTTPERAEWAMVYSDD
                     FWVDRQAALTERFNAWASQ"
     gene            4975..5427
                     /locus_tag="P5558_19985"
     CDS             4975..5427
                     /locus_tag="P5558_19985"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRY26659.1"
                     /translation="MADKLTLAGIETYRGVAYPWLCDGMGHVNVQFYGTLYDGASWHF
                     LSKVAPAAELGPNGLGWADVRQLIEYKREVQSGTLLVVRTRLVRLGTKSVEYAHGLHD
                     AEDGVLYSTNATVTVLFDLEARRAAPLTEAMRARCTKLLAGTGDGAAP"
     gene            5424..>6724
                     /locus_tag="P5558_19990"
     CDS             5424..>6724
                     /locus_tag="P5558_19990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY26660.1"
                     /translation="MIERADVVVIGAGVVGASVAWHLAQAGNSDVVLLERSKIGSGTT
                     WHAAGNMETWRADPLLGDMVDYTVRLFPELEADSGVAFGWRQTGRVHFTADPRVMESY
                     RAIPARARARGVEVELLDAGGVGEKLPILSIDGLEGGLWTPNDGRVDPTNLATAYARG
                     ATKRGVRLVEGAPVTRILVADGRVTGVETAQGPIRCEAVVLAGGLWSTSIARSCGVAL
                     PLVGVQHFYLLTKAIEGLSRDLPLFLSYDELLYGREDVGGLLLGVFDKNAIPVEAEDL
                     PGDFSFALLDEDWEQIAPNLEILQRRFPLLATTGIRAHVNGPESFTPDGNMLLGELDE
                     PSGLHLACGMNSNGIALAAGVGLLTAERVLGRPSSLDATRLDPRRFAGFQGGRRYRHA
                     RMSEIPTAVCIGEDAASPLRGIRRSPLHARHVADGAVLEAVA"
BASE COUNT          992 a         2209 c         2560 g          963 t
ORIGIN      
        1 ctaggagggc gtccatgccc gcgaaggaca tcgccgagct gctggcctgt cgcttcggcg
       61 aggtgccgga gctggcgccg gaggtggcgg gggtgccgct ggccgaagcg ctggcggggc
      121 gggcgtcgtg ccgggctttc gcgccgcggc cggtggatgt ggcgctgctg cggagcctcg
      181 cggcgctggc cttgtgcgcg ccttcgaaga gtgacctgca gcaggcggat atcgtgctcg
      241 ccaccgaccc ggaactaagg gcggccctgg cggcgaccgt gccgggccag gcctgggtgg
      301 cagggacgcc ggcgctcctg gtggtctgcg gcaacaaccg gcgccagcgg caggtcagcg
      361 agatgcgcgg tcgggagttc gccaacgatc acctggatgc cttcttcaat gcggccgtgg
      421 atggcggcat cgtgctggcc acgctggtcc tggcggcgga ggcggcggga ctcggcgcct
      481 gcccgatcag cgggctgcgc gaccggccgg aagaggtctc ggacctgctc ggcctgccgg
      541 cgcatgtctt cccgctcgcc gggctggcgc tgggctatcc ggtccgggag ggcaggatct
      601 cgccgcgcct gccgctcgag gtgacggtgc acgagaaccg ctggtcggag gcggggacgg
      661 ccgggcgcat ggccgcctat gacgcccgcc gccgcgagct ccagccctat gccagccagc
      721 gcggcctcga ccggttcggc gaggtctcgg actatggctg gctcgaggac aaggcccggc
      781 aatatgcgga gccgcagcgc gccgatttcg gggcgttcgt ccgccgccgc ggcttccggc
      841 tcgactgacc ccggctacgg cgtcgtgccg cgcaggcggg cgctgcggcg gcgcaggact
      901 tccacggtgg cgaggagcgt ggtcgagagg accagcagca tgacggcgac ggcggcaatc
      961 gccgggctga catattcgcg gacacccgag aagatctggc gcggcagggt gcgctggtcg
     1021 ggtgcggcga ggaagagggc gacgacgatc tcgtcgaagc tggtggcgaa ggcgaagagg
     1081 ccgcccgtgg cgacaccggg caggatgagc ggcagcatga cccggcggaa cgccacgagc
     1141 ggcggggcgc cgaggccggc ggcggcgcgc atcagccagg gatcgaagtt ctcgagcgtg
     1201 gccgcgaccg tgatgacgac gaagggtgcg ccgagcatgg cgtgggccag gatcagcccg
     1261 agataggtgc cgacgaggcc gatctggacg aagaagaagt agaccgcgac ggcggtgatc
     1321 acgatcggca cgatcatggg cgagagcacc agcatcatga tcaggcgctt gccccggaat
     1381 gtcgtgccgg cgaggccgag cgctgccggc acgccgacga gcagggccaa gagcgtggcg
     1441 aagcagccga cgaaaaggct gttggccgcg gcgtcccgcc aggcctggct gttcagcacc
     1501 tcgagatacc actggatcga ccagcccggc atggggaaga cgagctgcga gccggcggtg
     1561 agcgagatcg ggatgacgac gaggatgggg gcgaggagga agacgaggac gaggcccgtg
     1621 atggtgcggg ccagccagtg ccagccccgc tcctcgagcc tgcggcggaa gcccgccatc
     1681 agtggccgac caggcgcgtg gcgccgagca ggcggccgag aaacgccacg gcgaggagcg
     1741 tggcgaggag gaggaggctg ccgagggcgg cggccatgcc ccagttgccg gcctcgttga
     1801 gctggtaggt gacgaaccag ctcagcatct gatcgccggg gccgccgacg agggccgggg
     1861 tgatgtagta gccgagcgac atgatgaaga cgaggagcgc tccggcgccg acgccgggca
     1921 gggtctgcgg cagccagacg cggaggaagg cggccctggg ggcggcgccg agcgacaggg
     1981 cggcgcgcat ctgctcggcc gggatggtgc gcatcacggc gtagagcggc aggatcatga
     2041 agggcagctg cacgtggacc atggcggtca gcgtgccgcc gcggttgaag atgagctgga
     2101 gcggctccgc gatcagcccg agcgcctcga ggagcccgtt caccgggccg ttgccctgca
     2161 gcaggatcac ccaggcggtg gtgcggacga ggagcgacgt ccagaacggc aggagcaccg
     2221 cgatcagcag gaggttggtc gtgcgctggg gcagcaccgc gagcacggcg gcggtcgggt
     2281 agccgagcag caggcagagg gcggtgacct ggagcgcgat gacgaaggtc cgccccagca
     2341 gcatgcggta gagcgccatc tcgggcggca ccgcctcgac ctcgccggcg ctgtcgagct
     2401 gcaggtcgac ggtcgcgagc aggttcgatg gcgtgaccgt gcccgattcg ttgcgcaggc
     2461 tggtccacca gccgggctcg ccccacttgc cgtcgatggc gatcagcgtc tcccgccagg
     2521 agccgtccgg cacctcgggc agcttgttgg cggtcttcag gacgaggctg cggtagccga
     2581 cccgggcggc gttcagccgc ttgccgagga gcgccgcccg gccgccctcg cgggcgccgc
     2641 gcaggtcgag ggcgagggcg tcgaagacgg ggtccggcgg cagacccgtt cggtcccaat
     2701 gcacgatctc ggcgaccgtg cgcggcaggg tctcgggcac gatggcgttg tcgaccgcgc
     2761 gccagaggaa gaggacgatc ggcccggcga aggtgacgag cacgaacagc agcagcggca
     2821 ggagcaggcc ggcgcggcgc agaagctgcc ggcgtcgctg ggcggcgaag gcgcggcggg
     2881 tcaggtgcgg gtcatggcgt agcatgtgat caccagccat ggtatgccat ggtgctcaat
     2941 caagacgccg acgcggcgcg gaagagtctc ggatgcgggc atgaccacga ggcagttgcc
     3001 ggacgaatcg gggcccgggc cattcccggt ccagcgcctg cagcgtgcca cgctcaacga
     3061 cgaggtctat cacgagctca ggcgggcgat catgtccggg gccatcgagc ccggctcgac
     3121 catgaccatc cgcgggctcg cccagtcctt cggcatcagc ctgatgccgg tgcgccaggc
     3181 cctcaaccgg ctggtcgccg agcacgtcct cgtcctcctc cccaatcgct cggtggcgct
     3241 gccgcgcatc gatgcggcac ggtttcgcga gatcacccgc attcgcgtgg cgctcgaggg
     3301 gctcgccgcc gaggaggccg tggcgcatgt ggatgcggac gcgattgccc ggatggcggc
     3361 ggccaatgtg gcgatcgagg ccggggcggc gccgggcgag gcgctggcgc tcaatcgcga
     3421 gttccatttc acgctctatg ccgcctcggc gatgccgacc ctggtcgcca tgatcgagac
     3481 cacctggctg caggtcgggc ctttgctgca cgtgccgatc agacggatga tggacggtgc
     3541 ggctccccgt tccctgccgg cgcatcgccg ggtgctggcc ggcctgcgcg ccggggacgc
     3601 ggccgaagtg cgggatgcga tcagggcgga cctcgaggac gcggcggtcg acatcgccgc
     3661 cacgctcgac tgacgcccga gggtcggtca gccccgttcc ccgctgcgag tcccccgagg
     3721 tcgccccggc ctactggctg gcccaggcgt tgaagcgctc ggtcagggcg gcctgccggt
     3781 cgacccagaa gtcgtcgctg tagaccatgg cccattcggc gcgctcgggc gtggtcggca
     3841 gctcggcctt cacctcgtcg gacatcagcg cataggcctc cctggagggc acgccgtagc
     3901 tcagatgctt gaccagctcg gcctgggtct ctggacggct gtagaactcg atcagctttg
     3961 ccgcggggcc ggggttcggg ccgccctcga tctgcaccca gtagtccgtg ccgacccaga
     4021 agccggcggg ccaggcgatg acgaggttgc gctggttgtc gcgcgaggca ccggtgaccc
     4081 gggcattgaa gttgatggtc atgacgacgt cgccggcgag caggcgctgc atgccgtcgg
     4141 cggggctctc ggcccagagg aggtcgggct tgatctcgtc gagcttggcg aaggcgcgat
     4201 cgacgccctc gggcgtggcc agcacgtcgt agatctggtc gccgggcacg ccgtcggcga
     4261 cgagcgctat ctcgagcatg atccgggcgt ccttgtaggc gccgcgcttg cccgggaatt
     4321 tcgtggtgtc ccaatagtcc tgccagctcg tcgggcccgg gttcagcttg tcggcgtcgt
     4381 aggcgagcac ggtggcccag acgtcggagg cgatgccgca atcgtgggcg gcgtagtcga
     4441 gctggttgtc gcggccgccc aaggcgtccc agtcgagcgg gacgaaggcg ccctcgtcgc
     4501 agccctggat cacctgcggg ctctcgaagg cggcgaggtc ccaggtgatc ttgccggcct
     4561 cgatctgggc cgtgagcttg gcgtactcgc cgccgaactc ctcctcgacg accttgatgc
     4621 cggtctccgc ggcgaaaggc tcgaacaggg cctttcggta cgcatcctgc cagacgccgc
     4681 cccagccgac gacgatcacc tgctcctggg ccgcggccgt tccggccatg aggccagccg
     4741 cgaggaaagt ggccacgccg ccgagaacaa cgctcctgag ccgtgcctgc atcgaaccct
     4801 cccattgccc ccggatgggc gtttcgcctc ttgtgatcac atgctatggc agggcaagcc
     4861 gtgatgcaag cacggccaga cggtgaaaaa ggccggggag aagcggcgat ggcgaccctg
     4921 cggcggcgcg tgccatgctg gcgctgaagg gcgacgacca ggtgggtttc tgaggtggcc
     4981 gacaagctga cgctggccgg catcgagacc tatcgcggtg ttgcctaccc atggctctgc
     5041 gacggcatgg ggcacgtcaa tgtgcagttc tatgggacgc tctatgacgg ggcgagctgg
     5101 cacttcctct cgaaggtggc acccgcggcc gagcttgggc cgaacgggct gggctgggcg
     5161 gatgtgcgcc agctcatcga gtacaagcgg gaggtccagt cgggcacgct gctcgtggtc
     5221 cgcacgcggc tcgtgcgcct cggcacgaag tcggtcgagt acgcgcacgg gctccacgat
     5281 gctgaggatg gcgtgctgta ttcgaccaat gcgacggtca ccgtgctctt cgacctcgag
     5341 gcgcggcggg cagcacccct gacggaggcg atgcgcgccc gctgcacgaa gctgctggca
     5401 ggcaccggcg atggagccgc gccatgatcg agcgtgcgga tgtcgtcgtc atcggcgccg
     5461 gcgtggtggg ggcgagtgtc gcctggcacc tggcgcaggc ggggaacagc gacgtggtgc
     5521 tgctcgagcg ctcgaagatc ggctccggca ccacctggca cgcggccggc aacatggaga
     5581 cctggcgcgc cgatcccctg ctcggcgaca tggtcgacta caccgtccgc ctctttcccg
     5641 agctcgaggc ggacagtggc gtcgccttcg gctggcggca gacggggcgg gtgcacttca
     5701 ccgccgaccc tagggtcatg gagagctacc gcgccattcc ggcacgggcg cgggcgcgcg
     5761 gcgtcgaggt cgagctgctc gatgccgggg gcgtcggcga gaagctgccg atcctctcga
     5821 ttgacgggct cgagggcggg ctctggacgc ccaatgacgg ccgggtggac ccgaccaacc
     5881 tcgccaccgc ctatgcgcgc ggcgcgacga agcgcggcgt gcggctggtc gagggcgcgc
     5941 cggtcacccg cattctcgtg gcggacggac gggtcaccgg cgtggagacg gcacagggcc
     6001 cgatccgctg cgaggccgtg gtcctcgccg gcggcctgtg gtccacctcg atcgcccgga
     6061 gctgcggggt ggcgctgccg ctggtcggcg tgcagcattt ctacctgctc accaaggcca
     6121 tcgagggcct gtcgcgagat ttgccgctct tcctttccta tgacgagctg ctctatggcc
     6181 gcgaggacgt gggcggcctc ctgctcggcg tcttcgacaa gaacgctatt ccggtcgagg
     6241 ccgaggacct gccgggcgac ttctcctttg ccctcctgga cgaggactgg gagcagatcg
     6301 cgcccaatct cgagatcctg cagcggcgct tcccgctgct ggcgacgacc gggatccgtg
     6361 cccacgtcaa tggcccggag agcttcacgc ccgacggcaa catgctgctc ggcgagctgg
     6421 acgagcccag cggcctgcat cttgcctgcg gcatgaactc gaacggcatc gcgctcgccg
     6481 ccggcgtcgg cctgctcacg gccgagcggg tgctgggccg cccgtcctcg ctggacgcga
     6541 cccggctgga tcccaggcgg ttcgccggct tccagggcgg tcgccggtac cgccacgcgc
     6601 gaatgagcga gattccgacg gccgtctgca tcggcgagga cgcggcctcg ccgctgcgcg
     6661 gcatccgccg ctcgccgctg cacgcccggc atgtcgcgga cggggcggtc ctcgaggccg
     6721 tggc
//
LOCUS       DAOTFI010000249         6669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13624_length_6669_cov_7.390989, whole genome shotgun sequence.
ACCESSION   DAOTFI010000249 DAOTFI010000000
VERSION     DAOTFI010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13624_length_6669_cov_7.390989"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="P5558_19995"
     CDS             complement(<1..929)
                     /locus_tag="P5558_19995"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRY26661.1"
                     /translation="MTPPNRASAETRAVQALHEIEAATGAVVPAIQPSSTFARDADYA
                     LRDGFVYSRDTCPTVRHAERIVASLEDGAAALLFGSGLAACATLFETVPAGKRIAAPR
                     IMYHGVQDWLVRLRDRHGIGLDFFDAADPASLAAGVHPGETALLWLETPVNPSWDVID
                     IEAAAAIGHAAGALVAVDGTCASPVTTQALNLGADIVFHSATKYLNGHSDLTAGVLVT
                     KVQDESWRELTWLRSKLGGVLGAFEAWLLIRGMRTLYLRFERASASALAIARHLEGHP
                     AIERVLYPGLESHAQHAIARRQMTRGFGGMLSIL"
     gene            complement(926..1282)
                     /locus_tag="P5558_20000"
     CDS             complement(926..1282)
                     /locus_tag="P5558_20000"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRY26662.1"
                     /translation="MAIFEPFDVVAVPFPYVERPVTQRRPAVVLALPPSDNRHPLLWV
                     MMITSAANRHWPGDIPIVDIDTAGLSRPSLIRSAKIAVIEQIGIERKGRLADEDAAGL
                     RRALRTLMSPLLGTSP"
     gene            complement(1269..1496)
                     /locus_tag="P5558_20005"
     CDS             complement(1269..1496)
                     /locus_tag="P5558_20005"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY26663.1"
                     /translation="MRTTEVTARVSTKAQLVLPKRIREKLGINPGDLVRFVERDGAVV
                     IERLEREPAEDPFALFDEWSSAADAEAYGDL"
     gene            complement(1585..2343)
                     /locus_tag="P5558_20010"
     CDS             complement(1585..2343)
                     /locus_tag="P5558_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY26664.1"
                     /translation="MTHAEKVAANRLEGGRLDGLAIGEDEGYAVQQAANAALAKRLGE
                     VAGHKIGATAEGMRKLLNVPGPIAGDVFASTVHQKRATLRLADFVHPGIETEIAVRIA
                     RDLTPAEAPWTKERIAAAVAAVLPAIEIVDNRYVDFKEAGAGTIIADNAFNAGSILGR
                     ETTDWQHLPLDRLTARTLIDGKEVGTGLSDELLGHPLNALVWLADRYAGLGWTLKAGR
                     FVSLGTITPVQWITGPCEVQIEVEGLGGVEVVFG"
     gene            2431..3702
                     /locus_tag="P5558_20015"
     CDS             2431..3702
                     /locus_tag="P5558_20015"
                     /inference="COORDINATES: protein motif:HMM:NF009558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine
                     deacetylase/succinyl-diaminopimelate desuccinylase family
                     protein"
                     /protein_id="HRY26665.1"
                     /translation="MDGKLERAIGEREAAVVELTRELVAFPTVNPPGEAYTPCAEFLG
                     RRLAASGFEIAYIRGEGAPGDSDLYPRTNVVGRHETGRPGPTIHFNGHLDVVEVGQGW
                     TVDPFAGEVRDGRIHGRGTCDMKGGLAAAVVAVEALLASGLPLRGAIEISGTVDEESG
                     GFGGVAHLAGLGYFSKPRVDHVIIPEPLNVDRVCIGHRGVWWAEIETRGRIAHGSMPF
                     LGDCAIRHMGAFIQALETELYPRLGERRTAMPVVPDGARASTLNLNAIRAGTAPEKGG
                     LPSPCVADRCRLTIDRRYLIEERIEDVEGEIRALLDRLAAERPGFAYGLRELMHVAPI
                     MTEPTAPVPQAVAMAIEAVLGRPAAFVASPGTYDQKHIARIGHLDDCVAYGPGILDLA
                     HQPDEFVVIEDLVASMRVMALATLRLMGVPA"
     gene            3767..4000
                     /locus_tag="P5558_20020"
     CDS             3767..4000
                     /locus_tag="P5558_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRY26666.1"
                     /translation="MLFLLIGILAGWIAGKLIQGGGFGLIGNMVVGVVGAVLGGYVLG
                     AVGIDLGGFFGQLVTAVLGALILLFGVGLVKRA"
     gene            complement(4010..4612)
                     /locus_tag="P5558_20025"
     CDS             complement(4010..4612)
                     /locus_tag="P5558_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRY26667.1"
                     /translation="MTGRATLLDPAWRYVEKPEMAQFRAADWQVLEPQKRVYYQHSRA
                     DHVLRLLRTLEADPSWGYEINIFEHCLQSATMLLRDGHDEETVVVGLLHDIGFTICPE
                     THGAFAGALLAPYVDPRHVWMLERHGIFQLHHCHEHPDADPHERERWRGHPHFEWAAE
                     YVERYDQNAIDPAVGWAPLATFEPMVRRVFAAPRYRVTPP"
     gene            complement(4609..5601)
                     /locus_tag="P5558_20030"
     CDS             complement(4609..5601)
                     /locus_tag="P5558_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HRY26668.1"
                     /translation="MAYASARTVDVASLPVIDLDPLMSGTVTGLERTAAALTDAAERV
                     GFFYVTNHGVPASLIEEAAAVSRRFFDSPREAKATVAVQGRHRGWLDPGEAVMEGARR
                     PDLKESFIWGIEVEPDDPALATNPLLAPNRWPAFLPALQPTLGAYLDAAHACGWQLLR
                     AFAHALGVPPGHFIAHIDKPVSRGGTIYYPPQPATAGADQFGVAPHTDFGCLTLLWQD
                     MVGGLQVQAHDGGWVAAPPLADCFVVNVGDLLARWTNDRFRSTPHRVVNTSGRSRQSI
                     AVFVDPNAEQEVVPVCAEGEPARYPPVTVGEHIERRYNASFAYRQRQAQAQAQS"
     gene            5720..>6669
                     /locus_tag="P5558_20035"
     CDS             5720..>6669
                     /locus_tag="P5558_20035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRY26669.1"
                     /translation="MRYADLPPLATLRVLEAAVRLGSFTAAADELGRTQSAVSHQIKE
                     LELRLGCPLFERRGRGVVATAEGERLAEAVIGGLGRIHHAFDALRRERRADRVVVSAL
                     PGFVVKWLFPRLVRFDERHPAIEVDVMASGRRSELVAGEADLAIRYGSGSYPGLGSTL
                     LLGERMAPACAPRLARQLREPADLAAMTLLHDDLPTGEGVGVGWARWLAAVGLGGLEP
                     AHNRRFGQSNMVIDAAVEGVGVALGRSPLVAGDIRAGRLVVPFGPALATGLAYHIVGV
                     ERVLERPAVCAFIDWLVEEARAEPAPEVILAPNGLAPAGA"
BASE COUNT          918 a         2175 c         2560 g         1016 t
ORIGIN      
        1 aggatcgaga gcatgccgcc gaagccccgg gtcatctggc gcctggcgat ggcgtgctgg
       61 gcatggcttt cgaggcccgg gtagagcacg cgctcgatgg ccgggtggcc ctccaggtgc
      121 cgggcgatgg cgagggcact ggccgaggcg cgctcgaagc ggagatagag cgtgcgcatg
      181 ccgcgaatga gcagccaggc ctcgaaggcg ccgagcacgc cgccgagctt cgagcgcagc
      241 caggtgagct cgcgccagct ttcgtcctgg accttcgtga ccagcacgcc ggcggtgagg
      301 tcgctgtggc cgttcaggta cttggtggcg gagtggaaga cgatgtcggc acccaggttc
      361 agcgcctggg tcgtcaccgg cgaggcgcag gtgccgtcga cggcgacgag ggcgccggcc
      421 gcgtggccga tggcggcggc cgcctcgatg tcgatcacgt cccagctcgg gttgaccggc
      481 gtctcgagcc agaggagcgc cgtctcgccg ggatgcacgc cggcggcgag gctcgccggg
      541 tcggcggcgt cgaagaagtc gaggccgatg ccgtgccggt cccgcagccg caccagccag
      601 tcctggacgc cgtgatacat gatgcggggt gccgcgatcc tcttgcccgc cggcacggtc
      661 tcgaaaagcg tggcgcaggc ggcgagcccg gagccgaaga ggagggccgc ggccccgtcc
      721 tcgaggctgg cgacgatgcg ctccgcgtgg cgcacggtcg ggcaggtgtc gcggctgtag
      781 acgaagccgt cgcggagcgc gtagtcggcg tcgcgggcga aggtcgagga gggctggatg
      841 gcgggcacga cggccccggt ggcggcctcg atctcgtgca gcgcctgcac ggcgcgggtc
      901 tcggcggagg cgcggttggg tggggtcatg ggcttgtacc gagaaggggg ctcatcagtg
      961 tccgcaaggc tcgccgcaga ccggccgcat cctcatctgc cagacggccc tttctctcga
     1021 tgccaatttg ctcgatgacg gcaatcttgg ccgatcggat caacgagggg cgcgacaggc
     1081 cggctgtatc gatgtcgaca atcgggatgt cgccgggcca atgtcgattg gcggcactgg
     1141 tgatcatcat gacccagagc agtgggtgcc gattgtcgct gggcggcagt gccagcacga
     1201 cagccggtcg ccgctgcgtg accggccgct cgacatacgg aaacggcacg gccaccacgt
     1261 cgaacggctc aaagatcgcc ataggcctcg gcatcggctg ccgacgacca ctcgtcgaac
     1321 aacgcgaacg gatcctccgc aggctcgcgc tccagccgct cgatcacgac cgcgccatcc
     1381 cgctcgacga agcgaacgag atcgccggga ttgatgccga gtttttcgcg gatgcgcttc
     1441 ggaagcacga gctgcgcctt cgtgctgacc cgcgccgtga cttcggttgt gcgcatgatg
     1501 accttgggta agacgaccta cttcttactt ggggcgtttt aaccgggcca caagggagag
     1561 ttgccgtcgt cgcggtaaac tcgactagcc gaacaccacc tccacacccc ccagcccctc
     1621 gacctcgatc tgcacctcgc aggggcccgt gatccactgg acgggggtga tggtgccgag
     1681 gctgacgaag cggccggctt tcagggtcca gccgaggccg gcgtagcggt cggcgagcca
     1741 gacgagggcg ttgagcgggt ggccgaggag ctcgtcggag aggccggtgc cgacttcctt
     1801 gccgtcgatc agggtgcggg cggtgaggcg gtcgaggggg aggtgctgcc agtcggtggt
     1861 ttcgcggccg aggatgctgc cggcgttgaa ggcgttgtcg gcgatgatgg tgccggcacc
     1921 ggcctccttg aaatcgacgt agcggttgtc gacgatctcg atggccggca ggacggcggc
     1981 gaccgcggcg gcgatgcgct ccttcgtcca gggggcctcg gcgggcgtga ggtcgcgggc
     2041 gatgcggacg gcgatctcgg tctcgatgcc tggatggacg aagtcggcga gacggagggt
     2101 ggcgcgcttc tggtgcacgg tcgaggcgaa gacgtcgccg gcgatggggc cggggacgtt
     2161 gaggagcttc cgcatgcctt cggcggtggc gccgatcttg tggccggcga cttcgccgag
     2221 ccttttggcg agggcggcgt tggcggcctg ctggacggcg tagccctcgt cctcgccgat
     2281 ggcgaggccg tcgagccggc cgccttccag gcgattggct gcaaccttct cggcatgggt
     2341 catggtgagc ctccctccgg tcgttgaccc tgccgcgatt gacagggccg cctcgacacc
     2401 cgacgctccc ccgtcataac gggggctggc atggacggaa agctggagcg ggcgatcggc
     2461 gagcgcgagg cggcggtggt cgagctgacc cgcgagctcg tggcctttcc caccgtcaac
     2521 ccgccgggcg aggcctacac gccctgcgcc gagttcctcg ggcgcaggct cgccgccagc
     2581 ggcttcgaga tcgcctacat ccggggcgag ggggcgccgg gcgacagcga cctttatccc
     2641 cggaccaacg tcgtcggccg ccatgaaacc ggccgaccgg ggccgaccat ccatttcaac
     2701 ggccatctcg acgtggtcga ggtggggcag ggctggacgg tcgacccgtt cgccggggag
     2761 gtccgggacg ggcggatcca cgggcgcggc acctgcgaca tgaagggcgg gctggcggcg
     2821 gcggtggtgg cggtcgaggc gctcttggcc tcggggctgc cgctcagggg tgcgatcgag
     2881 atctcgggca cggtggacga ggaatcgggc ggcttcggcg gtgtcgccca cctggcgggg
     2941 ctcggctatt tctcgaagcc ccgggtcgat cacgtgatca ttcccgagcc tctcaacgtc
     3001 gatcgggtgt gcatcggcca ccgtggggtg tggtgggcgg agatcgagac ccgtgggcgg
     3061 atcgcgcatg gctcgatgcc ctttctcggc gactgcgcca tccgccacat gggggccttc
     3121 atccaggcgc tggagaccga gctctatccc aggctcggcg aacggcgcac cgccatgccg
     3181 gtggtgccgg acggggcacg ggcctcgacc ctcaacctca atgcgattcg ggccggcacg
     3241 gcgccggaga agggcgggct gcccagcccc tgcgtggcgg atcgctgccg gctgaccatc
     3301 gaccggcgct atctcatcga ggagcgcatc gaggacgtcg agggcgagat cagggccttg
     3361 ctcgaccggc tggctgccga gcggccgggc ttcgcctatg gcctgcgcga gctgatgcac
     3421 gtggccccca tcatgaccga gcccaccgcc ccggtgccgc aggcggtggc catggcgatc
     3481 gaggcggtgc tcggccggcc ggcggcgttc gtcgcctcgc ccggcaccta tgaccagaag
     3541 cacatcgccc ggatcgggca cctcgacgac tgcgtcgcct acgggcccgg cattctcgac
     3601 ctggcgcacc agccggacga gttcgtggtg atcgaggatc tggtggcgtc gatgcgggtc
     3661 atggcgctcg ccacgcttcg gctcatgggc gtcccggctt gaccggcggc atgcgagcgg
     3721 cgaggctcaa gcgacaacgc agatggaggc cctggccttg ggtatcgtgc tctttctcct
     3781 gatcggcatc ctcgccggct ggatcgccgg caagctcatc cagggcggcg gcttcgggct
     3841 gatcggcaac atggtggtgg gcgtggtcgg cgccgtgctc ggcggctacg tgctgggggc
     3901 ggtcggcatc gatctcggcg ggttcttcgg ccagctcgtc acggcggtgc tgggggcgct
     3961 catcctgctg ttcggcgtcg gcctcgtaaa acgggcctag ttcccgacct cagggcgggg
     4021 taacgcggta gcgcggcgcg gcgaagacgc ggcggaccat gggctcgaag gtggcgagcg
     4081 gcgcccagcc gacggccggg tcgatggcgt tctggtcgta gcgctcgaca tactcggcgg
     4141 cccactcgaa atgcgggtgg ccgcgccagc gctcgcgctc gtgcgggtcg gcgtccgggt
     4201 gctcgtggca gtggtggagc tggaagatgc cgtgccgctc gagcatccag acatgcctgg
     4261 ggtcgacata gggggcgagg agcgctccgg cgaaggcgcc gtgcgtctcc gggcagatgg
     4321 tgaagccgat atcgtgcagc aggccgacga cgacggtctc ctcgtcgtgg ccgtccctga
     4381 gcagcatggt cgccgactgc aggcagtgct cgaagatgtt gatctcgtag ccccagctcg
     4441 ggtcggcctc gagggtgcgc agcaggcgca gcacgtgatc cgcgcgcgag tgctggtagt
     4501 agacgcgctt ctgcggctcc agcacctgcc agtcggcggc gcggaactgc gccatctcgg
     4561 gcttctcgac atagcgccag gcggggtcga ggagggtggc gcggccggtc atgactgcgc
     4621 ctgcgcctgg gcctggcgct gccgataggc gaaggaggcg ttgtagcggc gctcgatatg
     4681 ctcgccgacc gtgaccggcg ggtagcgggc gggctcgcct tcggcgcaga ccggcacgac
     4741 ctcctgctcg gcgttcgggt cgacgaagac ggcgatggac tggcgcgagc ggccggaggt
     4801 gttgaccacc cggtgcggcg tcgagcggaa gcggtcgttg gtccagcggg ccaggaggtc
     4861 gccgacattg acgacgaagc agtcggccag gggcggcgcc gccacccagc ccccgtcatg
     4921 ggcctggacc tgcagcccgc ccaccatgtc ctgccagagc agggtgaggc agccgaaatc
     4981 ggtgtgtggc gccacgccga actggtcggc gccggcggtc gccggctgcg gcgggtagta
     5041 gatcgtgcca ccccggctca ccggcttgtc gatatgggcg atgaagtggc ccggcggcac
     5101 gcccagcgca tgggcgaagg cgcggagcag ctgccagccg caggcatggg cggcgtcgag
     5161 ataggccccg agcgtcggct gcagcgcggg caggaaggcg ggccagcgat tgggggcgag
     5221 gagggggttg gtggccagcg ccgggtcgtc cggctcgacc tcgatgcccc agatgaagct
     5281 ctccttcagg tcggggcgcc gggcaccctc catcaccgcc tcgccgggat cgagccagcc
     5341 gcgatggcgg ccctggaccg ccaccgtcgc cttggcctcg cggggggaat cgaagaagcg
     5401 gcgcgagacc gccgcggcct cctcgatcag ggacgccggc acgccgtgat tggtgacgta
     5461 gaagaagccg acacgctccg cggcatcggt cagggcggcg gccgtccgct ccagccctgt
     5521 gacggtgccg ctcatgagtg ggtcgaggtc gatgacgggc agcgaggcca cgtcgaccgt
     5581 gcgggctgac gcataggcca tggtggagct tccggtatgc ttgtgccgag ggccgggacg
     5641 gtgcaagtct cgctcttctg gaccggccga tcaatatgcc gtaattcatt cgtggtatga
     5701 gttctgctca tgggtcgtca tgcgctacgc cgatctgccg ccgctcgcca cgctccgggt
     5761 cctcgaggcc gcggtccggc tcggcagctt cacggcggcg gcggacgagc tcggccggac
     5821 gcagagtgcg gtcagccacc agatcaagga gctggagctc aggctcggct gcccgctctt
     5881 cgagcggcgc ggccgcggcg tggtcgccac ggccgagggc gagcgcctcg ccgaggcggt
     5941 gatcggcggg ctcggccgca tccaccacgc cttcgacgcg ctgcgccgcg agcgccgggc
     6001 cgaccgggtg gtggtcagcg cgctgcccgg cttcgtcgtc aagtggctct tcccgcggct
     6061 ggtccgcttc gacgagcggc acccggcgat cgaggtcgac gtgatggcga gcggccggcg
     6121 cagcgagctg gtggcgggcg aggccgacct cgccatccgc tacggcagcg gcagctatcc
     6181 gggcctcggc tcgaccctgc tgctgggcga gcgcatggcg ccggcctgcg cccccaggct
     6241 ggcccgccag ctccgcgagc cggccgatct cgctgccatg acgctgctgc acgacgacct
     6301 gccgaccggc gagggggtcg gcgtgggctg ggcgcgctgg ctggcggcgg tcggcctggg
     6361 cggcctcgag cccgcgcaca accggcgctt cggccagtcc aacatggtga tcgacgcggc
     6421 ggtcgaggga gtcggggtag cactcggccg cagcccgctg gtcgccggcg acatccgggc
     6481 cgggcggctc gtcgtgccgt tcggcccggc gctcgccacc gggctcgcct atcacatcgt
     6541 cggcgtcgag cgggtgctcg agcggccggc ggtctgcgcc ttcatcgact ggctggtgga
     6601 ggaggcgagg gccgagccgg cgccggaggt cattctggcg ccaaacgggc tggcgccggc
     6661 cggggctca
//
LOCUS       DAOTFI010000250         6664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13635_length_6664_cov_7.919352, whole genome shotgun sequence.
ACCESSION   DAOTFI010000250 DAOTFI010000000
VERSION     DAOTFI010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13635_length_6664_cov_7.919352"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="P5558_20040"
     CDS             <1..1086
                     /locus_tag="P5558_20040"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HRY26670.1"
                     /translation="ADVAFAKGRVAALDDDIPAERAQNVVDVTDRLVTPGLIDLHTHV
                     YWGGTSLGVNADKLARRSGTTTFVDAGSAGPGNFPGFRHHVIERSRVRILAYMNISFA
                     GIFAFSKNVMVGENLDIRLCNPREVVAVAREHLEHLVGVKVRVGQVTSGSNGAGPFNA
                     ALDAADKLHMPLMTHLDYPPPGRHEIIEHLRKGDVLTHCFKPFPNAPIDGHRQIRADM
                     HSARKRGVVFDIGHGMGSLAFETATAMLEQGFLPDVISSDVHTLCIHGPAYDLLSTLS
                     KFLALGMRLDDVIRTATLHPAAAIGRNDLGSLAPGAIGDASVLERRPGRYVHTDSLGE
                     TITADEHLFCSGLVTAGSWWPSEETCA"
     gene            1083..2231
                     /locus_tag="P5558_20045"
     CDS             1083..2231
                     /locus_tag="P5558_20045"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRY26671.1"
                     /translation="MSTSRPSPNAALVGQLGSRLLLDTPALVLDLDAFELNLRTVHGR
                     AKERRIALRPHAKSHKSAAVGRAQLAAGAIGLCCAKLGEAEALAAAGLDGFLITAPVT
                     SRPKVQRLMGLNARLADLAVVVDDVANVAELAAATAEAGRQLKLLIDVDVGTHRMGVT
                     SPEAALALAKAIREADGLELVGVQGYAGQTQAIPSYAERRAASGKALAALADVRDALV
                     EQGHPCPIVTGSGTGTHDFDHEFGLFTDLQVGSYVFSDVIYNSIEIGAGGDRPVLPAL
                     FVVSRVISRQHRDFATIDAGSKSFSMDGPMPVLWQGPTPGSTYGRFGDQFGRLDLTGA
                     DRPVELGELTTWMVPHCDPTMNLYDHYHCVRGDDLVDIWAIEARGRTD"
     gene            2215..2952
                     /locus_tag="P5558_20050"
     CDS             2215..2952
                     /locus_tag="P5558_20050"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRY26672.1"
                     /translation="MAAPTEPQEQPRPARARLGRALRAIRNGLGISLAEVALRTGVSV
                     STLSKVENGQMSLTYDKLVQLSEGLGVDIGAFFDASPPAEVAGKGQVTARRSITRPGE
                     EMLVETPNYDYRYLCTDLSQKAMVPILITIRARSLVRFSQLSRHSGEEFIYVLRGPIE
                     VHTEHYAPARLNEGDALYIDSTMAHASLAIGDTDALVINVCYSPSAGHLQNLVDLARA
                     AALSADNEAADAPPVRGRASAVKLPRP"
     gene            complement(2949..4070)
                     /locus_tag="P5558_20055"
     CDS             complement(2949..4070)
                     /locus_tag="P5558_20055"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26673.1"
                     /translation="MLRLPRRRFLSATGGTVAALGLGSAGFAPGRAQAASGTVRWVSP
                     RGTIEVLDDYPYWVAKKYGWFGDIETTLEPGPMEATATIKLVDQEQSDMGFPSPGVFS
                     LGLEQGMPITSVWQMGAFDVFSFAFRPGEKPADPKELAGKTILLGSIGWKSIVDPELA
                     QLGIDPASVEYAEAGNLWGQALLQGQGDVALSWEGLRAQWTGQGLEFDYLLPYDFSRL
                     PANTFVVRRSDAEDAAKAELYEAYLRAWAMGLEFGYLNPRATTQIVMEQFPGLASTLA
                     PDIATESMMQLAKVFRGPWDQREGWGWHDMEQWQFFFDLLLEIGQITKPIVATDVCSN
                     AWVPAANDFDHAAVQAAADGFELSDDYAAVDVAVIESRL"
     gene            complement(4096..4947)
                     /locus_tag="P5558_20060"
     CDS             complement(4096..4947)
                     /locus_tag="P5558_20060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26674.1"
                     /translation="MASVDRELRDKIPTFEKAGAADVGNWSSAASTLSILKGSRDVTL
                     ILVTAIIVFGGAELLLRLFEVPAYIAPTPSQVVWALITDFPYIGPHIGYTLIELLVGF
                     SIGASIGFVLAAVITQFPLVEKIVAPYILLLVTTPMLALVPLLILKFGFGFTPRIIAV
                     TLASGPMVMINAATGFRRTDLAKIALARSFGASTLQIFLKIRIPMAMPMVIVGLMIGS
                     IFGLLTAVGAEMVGGSFGLGNRLTYYSSLIQMPQFFACILVLAVIGISIYVFFYWVGK
                     KWASWES"
     gene            complement(4963..5745)
                     /locus_tag="P5558_20065"
     CDS             complement(4963..5745)
                     /locus_tag="P5558_20065"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26675.1"
                     /translation="MSQNGQPKLTVANATKYYDTGTGRVHALDDFSIEVQEGELACLL
                     GPSGCGKSTLLWAMAGLHGLSSGEIRLDGKPVTKPNPQIAMVFQEANLLPWRNLIKNI
                     ELPFEIRGQRPDQARIDALLAEVGLTGFESKMPRELSGGMQQRASIVRALSVDPSLLL
                     MDEPFGALDAFTRDEMNLLIQQIWMETRKTIVFITHNIAEAVFLADRVFVMSARPGRL
                     ARIFEIDLPRPRNLEIFMAPHFIELVGEIKGSIDHKPQVQAA"
     gene            5887..>6664
                     /locus_tag="P5558_20070"
     CDS             5887..>6664
                     /locus_tag="P5558_20070"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRY26676.1"
                     /translation="MEATFNTIYGRNLICELRNFVHRPYLVVTMEELWERFEGAFDDG
                     LVGPVFASSMDVEMLEKALDGLPPAECIIGIGGGRAVDTAKYFAWRTRLPLFQIPTSM
                     SVNAVFGHRAGVRQNGQVRYMGYAVPEAVYVDFDVIQSAPAHINRSGVCEILCYHTGH
                     LDWAYADRLGKCEAKWPYDQRLVDAARQRFDLVMTHLDDINAVNETGIRALMEANRWG
                     GATFHNAGWNPRHIEGIDHFVFYALEYHTRRPFLHGQPVCL"
BASE COUNT         1165 a         2258 c         2130 g         1111 t
ORIGIN      
        1 gccgatgtcg ccttcgccaa ggggcgggtc gccgccctcg acgacgacat tccggccgag
       61 cgcgcccaga acgtcgtcga cgtgaccgac cgcctcgtca cgcccgggct gatcgacctg
      121 cacacccacg tctactgggg tggcacctca ctgggcgtga atgccgacaa gctcgcccgg
      181 cgcagcggca ccaccacgtt cgtggatgcc ggcagcgccg ggcccggcaa tttcccgggc
      241 tttcgccacc acgtcatcga gcgctctagg gtccgcatcc tggcctacat gaacatctcc
      301 ttcgccggca tcttcgcgtt ctcgaagaac gtcatggtgg gcgagaacct cgacatccgc
      361 ctctgcaacc cgcgcgaggt ggtggccgtc gcccgggagc acctcgagca cctcgtcggc
      421 gtcaaggtcc gggtcggcca ggtcaccagc ggcagtaacg gcgccggtcc cttcaacgcc
      481 gccctcgatg ccgccgacaa gctgcacatg ccgctgatga cccatctcga ctacccgccg
      541 cccggccgcc acgagatcat cgagcatctg cgcaagggcg acgtcctcac ccactgcttc
      601 aagccgttcc cgaacgcgcc gatcgacggc caccgccaga tccgcgccga catgcacagc
      661 gcccgcaagc gcggcgtcgt cttcgatatc ggccacggga tgggctcgct cgccttcgag
      721 acggcgaccg ccatgctgga gcagggcttc ctccccgacg tcatctccag cgacgtgcac
      781 acgctctgca tccatgggcc ggcctacgat ctgttgagca cgctgtccaa gttcctggct
      841 ctcggcatgc gcctcgacga cgtgatccgc acggccaccc tgcacccggc ggccgccatc
      901 ggccgcaacg atctcggcag cctcgcgccc ggtgccatcg gcgacgcctc cgtcctcgag
      961 cggcggcccg gccgctatgt ccacaccgac agcctcggcg agacgatcac cgccgacgag
     1021 catctgttct gcagcggcct cgtcaccgct ggatcatggt ggccgagcga ggagacgtgc
     1081 gcatgagcac gtccaggccc agccccaatg ccgcgctcgt tggccagctc ggctcccgtc
     1141 tcctgctcga caccccggcc ctggtgctcg acctcgacgc gttcgagctg aacctgcgca
     1201 ccgtgcatgg ccgggcgaag gaacgcagga tagcgcttcg gccgcatgcc aagtcgcaca
     1261 aatcggcggc tgtcggccga gcccagctgg cggccggcgc catcgggctc tgctgcgcca
     1321 agctcggcga ggccgaagcg ctggccgcgg ccggcctcga cgggttcctg atcaccgcac
     1381 cggtgaccag ccggccgaag gttcagcgcc tgatgggcct gaacgcccgg ctcgccgatc
     1441 tcgctgtcgt tgtcgacgat gtcgccaatg tcgccgagct ggcggcagcc acggccgagg
     1501 ccggcaggca gttgaagctc ctgatcgacg tcgatgtcgg cacgcaccgc atgggcgtca
     1561 ccagtcccga agcggcgctc gcgctggcca aggcgatccg tgaagccgat ggactcgagc
     1621 tcgtcggcgt gcagggctat gccggtcaga cccaggcgat cccgagctac gccgagcggc
     1681 gggcggcgtc gggcaaggcg ctggccgcgc ttgccgacgt acgcgacgcg ctggtcgagc
     1741 agggccatcc ctgcccgatc gtcaccggca gcggcaccgg cacgcacgac ttcgaccacg
     1801 agttcggcct cttcaccgac ctgcaggtcg gctcctatgt cttctccgac gtgatctaca
     1861 attcgatcga gatcggtgct ggaggagata ggccggtcct gccggcgctc ttcgtcgtga
     1921 gccgtgtgat cagccggcag catcgggact tcgccacgat cgacgcgggc tcgaagagct
     1981 tctcgatgga cgggccgatg ccggtcctgt ggcaggggcc gacgccgggc tccacctatg
     2041 gccggttcgg cgatcagttc ggccgtctcg acctcaccgg tgctgacagg ccagtggagc
     2101 tcggcgagct gaccacatgg atggtgccgc attgtgatcc gaccatgaat ctctacgacc
     2161 actatcactg tgtgcgcggc gatgatctcg tcgatatctg ggcgatcgag gcgcgtggcc
     2221 gcaccgactg agccacagga acagccgcgg ccggcgcggg cccggctcgg tcgcgcgctg
     2281 cgggcgatcc gcaacgggct cggcatcagc ctcgccgagg tggcgctccg caccggggtc
     2341 agcgtctcga cactgtcgaa ggtcgagaac ggccagatgt cgttgaccta tgacaagctg
     2401 gtgcagctca gcgagggcct cggggtcgac atcggcgcct tcttcgatgc gtcacccccc
     2461 gccgaggtcg ccggtaaagg ccaggtgacg gcccggcgga gcatcacccg accgggcgag
     2521 gagatgctgg tcgagacgcc gaactacgac taccgctacc tctgcaccga cctgtcgcaa
     2581 aaggcgatgg tgcccatcct gatcaccatc cgtgcccgtt cgctggtcag gttcagccag
     2641 ctcagccgac attcgggcga ggagttcatc tacgtgctgc gtggtccgat cgaggtgcat
     2701 acggagcact acgcgccggc ccgcctgaac gagggcgatg cgctctatat cgacagcacc
     2761 atggcgcacg cctcgctcgc gatcggcgac accgacgccc tggtgatcaa tgtctgctac
     2821 agtccgagcg cgggccacct gcagaacctg gtcgacctcg cccgagcagc agctctttcc
     2881 gccgacaacg aggcggcgga cgccccgccc gtgaggggcc gggcgtccgc cgtgaagctg
     2941 ccgaggccct agaggcggga ctcgatcacc gccacgtcca cggcggcgta gtcgtcgctc
     3001 agctcgaagc cgtcggccgc tgcctgaacg gccgcgtggt cgaagtcatt ggccgctggc
     3061 acccacgcat tgctgcacac atcggtcgcg acgatgggct tcgtgatctg cccgatctcc
     3121 aaaaggagat cgaagaagaa ctgccactgc tccatgtcgt gccagcccca cccctcgcgc
     3181 tggtcccagg gaccgcgaaa gaccttggcg agctgcatca tggactcggt ggcgatgtcg
     3241 ggtgcgaggg tcgaggccag gcccgggaac tgctccatga cgatctgggt cgtcgcccga
     3301 gggttgaggt agccgaattc caggcccatg gcccaggcgc ggagataggc ttcgtagagc
     3361 tcggccttcg ccgcgtcctc ggcgtccgag cggcggacca cgaaggtgtt ggccggcaac
     3421 ctcgagaaat cgtacggcag gagatagtcg aactcgagac cctggccggt ccactgcgct
     3481 cgcaggccct cccatgacaa agcgacgtcg ccctggccct gcagcagtgc ctggccccag
     3541 agattgccgg cttcggcgta ctcgacactg gccggatcga tgccgagctg ggcgagctcc
     3601 ggatcgacga tcgatttcca gccgatcgag ccgaggagga tggtcttgcc cgccagctcc
     3661 ttcggatcgg ccggcttctc gcccgggcgg aacgcgaagc tgaagacgtc gaaggcgccc
     3721 atctgccaga ccgaggtgat cggcatgccc tgctcgagcc cgagcgagaa gacgccgggt
     3781 gaggggaagc ccatgtcgga ctgctcctga tccaccagct tgatggtggc ggtcgcctcc
     3841 atcggcccgg gctccagcgt cgtctcgatg tcgccgaacc agccgtattt cttggcaacc
     3901 caatagggat agtcgtcgag cacctcgatc gtgccacgcg ggctgaccca gcggacggtg
     3961 ccgctcgccg cctgcgcacg gcccggggcg aagcccgccg acccgagccc gagagccgcc
     4021 accgtgccgc ccgtcgccga aaggaaccgc cgcctcggaa gcctcagcat ccctgtctct
     4081 cccttgttcg ggcgatcagc tttcccagct cgcccatttc ttgccgaccc agtagaagaa
     4141 aacatagatc gagatgccga tgaccgcgag cacgaggatg caggcgaaga actgcggcat
     4201 ctggatcagc gacgagtagt acgtcagacg gttgccgagt ccgaacgagc cgccgaccat
     4261 ctccgcaccc acggccgtca ggaggccgaa gatggagccg atcatcaacc cgacgatgac
     4321 catcggcatg gccatgggga tgcggatctt caggaagatc tgcagcgtcg aggcgccgaa
     4381 gctgcgcgcc agggcaattt ttgcgaggtc ggtgcggcga aagccggtcg cggcgttgat
     4441 catcaccatg ggcccggagg ccagcgtcac ggcgatgatg cgcggcgtga agccgaagcc
     4501 gaacttgagg atcaagagcg gcaccagggc cagcatcggc gtcgtcacga gcagcaggat
     4561 gtagggggcc acgatcttct cgaccagcgg gaactgggtg atcacggccg ccagaacgaa
     4621 gccgatcgag gcgccgatcg agaagcccac gagaagctcg atcagcgtat agccgatatg
     4681 cggcccgata tacggaaagt ccgtgatcag cgcccagacc acctgggatg gcgtcggagc
     4741 gatgtaggcc ggcacctcga acagccgcag caacagctcg gcaccaccga agacgatgat
     4801 cgcggtgacc aggatcagtg tcacatcccg gctgcccttg aggatcgaca gtgtcgaggc
     4861 ggcgctcgac cagttgccga catcagcggc cccggccttc tcgaaggtcg ggatcttgtc
     4921 ccggagctcc ctgtcgacgc tcgccatatc ggtctggtgc tcctaggccg cctggacttg
     4981 cggcttgtga tcgatcgagc ccttgatctc accgacgagc tcgatgaaat gcggcgccat
     5041 gaagatctcg aggttgcgcg gccgcggcag gtcgatctcg aagatacgcg ccagacggcc
     5101 cggcctggca ctcatcacga agacccggtc ggccaggaag accgcctcgg cgatgttgtg
     5161 cgtgatgaag acgatggttt tcctcgtctc catccagatc tgctggatca gcaggttcat
     5221 ctcgtcgcgg gtgaaggcat cgagggcgcc gaacggctcg tccatcagga gcagcgacgg
     5281 atcgaccgag agtgctctga cgatcgaagc ccgttgctgc atgccaccgg aaagctcgcg
     5341 cggcatcttg ctctcgaacc cggtcagtcc gacttccgcc agcagggcat cgatgcgcgc
     5401 ctggtccgga cgctggccgc ggatctcgaa cggcagctcg atgttcttga tgagattgcg
     5461 ccagggcagg agattggcct cctggaagac catggcgatc tgcgggttgg gcttcgtcac
     5521 gggcttgccg tcgaggcgga tctctccgga cgagaggccg tgcagaccgg ccatcgccca
     5581 gagcagcgtg gacttgccac agcccgaggg cccgagcagg caggcgagct cgccctcctg
     5641 cacttcaatc gagaaatcgt cgagcgcgtg cacccgcccg gtgcccgtat cgtaatattt
     5701 cgtcgcgttg gcgaccgtca gcttgggctg cccgttctgg ctcaaggcgc aggctccttt
     5761 gctcccagac accaacgtca ccgagtgcgt ggcattcgtc aaggtggtct gctcagcact
     5821 tgacgccttc gcaaggctct tcgacagtgc cactcgatgg atcagagacc agcgaagcga
     5881 ggcggaatgg aggcgacctt caacaccatt tatggtcgca atctcatatg cgagctcagg
     5941 aatttcgttc atcgcccgta tctcgtggtg accatggagg agctttggga acgcttcgag
     6001 ggggcgttcg atgatggtct tgtcgggcca gtcttcgcct cctccatgga tgtggagatg
     6061 ctggagaagg ctctggacgg tctgccgccg gccgaatgca tcatcgggat cggtggtgga
     6121 cgggcggtgg acaccgccaa gtatttcgcc tggaggacgc gcctgccgct ctttcaaatc
     6181 ccgacctcga tgtcggtcaa tgccgtattc ggacatcgcg ctggcgttcg ccagaacggt
     6241 caggttcgct acatgggcta tgccgtgccc gaggcggtct acgtcgattt cgacgtcatc
     6301 caatctgcgc cggcccacat caaccgtagc ggtgtatgcg aaattctctg ctaccacacc
     6361 ggccatctcg actgggccta cgccgatcgt ctcggcaaat gtgaagcgaa atggccctac
     6421 gatcagcggc tcgtcgatgc ggcccggcag cgcttcgatc tcgtcatgac ccatctcgac
     6481 gacatcaatg ccgtcaatga gacaggcatt cgcgcgctca tggaggcaaa tcgctggggc
     6541 ggcgccacct ttcacaatgc cggctggaac cctcgccaca tcgaaggcat cgaccatttt
     6601 gtcttctacg cgctcgaata ccatacgcga cggcccttcc tgcacggcca gccggtctgt
     6661 ctag
//
LOCUS       DAOTFI010000251         6613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13772_length_6613_cov_6.459896, whole genome shotgun sequence.
ACCESSION   DAOTFI010000251 DAOTFI010000000
VERSION     DAOTFI010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13772_length_6613_cov_6.459896"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1017
                     /locus_tag="P5558_20075"
     CDS             241..1017
                     /locus_tag="P5558_20075"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY26677.1"
                     /translation="MAPEEKNPLARQRLDGKVVCVTGGASGIGAAAARLMVAAGARVV
                     ILDRDDGAAEATAGAIGGGCEAMALDVASETDIDRVLADIAGRHGRIDVMVANAGINI
                     RRTAVDCSLDDWNAVLQVNLTGVFLSSRAAARHMPEKGGAVVVTASIMSFSGGGLYPN
                     ISYATTKGALVNLVRSMAVEWAPRNIRVNAVAPTWTKTRFIKPLTDDAELTAKLEALT
                     PMGRLAEPEEVAHAMLFLASDAASMVTGHTLAVDGGFLAQ"
     gene            1561..1941
                     /locus_tag="P5558_20080"
     CDS             1561..1941
                     /locus_tag="P5558_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY26678.1"
                     /translation="MTSRTKIGTPILDERKRVPARFYATPLGSEPVRDWLKGLAVDAR
                     RRIGFDLKQLEYGWPVGMPLCRPLGRSLWELRSSLPDGRIARVIFCVRDGELLLLEGF
                     IKKTQKTPPQEIELALQRMRGMGR"
     gene            1938..2252
                     /locus_tag="P5558_20085"
     CDS             1938..2252
                     /locus_tag="P5558_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY26679.1"
                     /translation="MTEIEDGRPGSTLDDFLAEEGFLEEATEHAVKSVLAWQIGEAMK
                     DRNLSKAAMAREMRTSRTQLDRLLDPDNGSVTLHTLHRAAAILGKRLRVDLVDGRPPG
                     ER"
     gene            complement(2677..3591)
                     /locus_tag="P5558_20090"
     CDS             complement(2677..3591)
                     /locus_tag="P5558_20090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRY26680.1"
                     /translation="MTDPSPAAVPPRRLLLLANPRARNGAAASDLVAPLAGLGLEVVF
                     VEPDGIEALLATIRTTGRTVDRIAIAGGDGSIAAALPALLDLQKPLAVLPLGTANDLA
                     RNLRLPDAREEQLRLVARGGVRRIDLASVNGRPVLNAVSIGLGAAVARMHQGTAKRLL
                     GVLNYPRVLYAAWRRIHPFRVEITCDGTVHRDRFIHVAVVNGRFHGGGIEPRPSGTIT
                     DGVLDLYALRADPVHRLVRAMAKLKIEGAESDEAFRLQGRHIAIATSRPRRANIDGEL
                     VGRTPLDIEIMPEALPVVVPGRAEHDGS"
     gene            complement(3701..5470)
                     /locus_tag="P5558_20095"
     CDS             complement(3701..5470)
                     /locus_tag="P5558_20095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY26681.1"
                     /translation="MATRRDLIAARSSMWFRGGTFRLRSGVVLALTVGVLLANGLFFY
                     NSHVRLAAATRDVAESVDARQLLSELLTVLLGAESGHRGFILTDEASNLAPYNAALQQ
                     ADELLGRIGPLLQADAPQMQRLAIVRALVAERFRELSRTLDARRQQGAEAARRLIRDN
                     QQLGTLDELRRVVGEMQDVEAVTLDTRRADWEEARFHAFAAFVVFLTVSAALVAALFG
                     FMRREVVDRIAYNRRLKAYADELGASLAELRTERNEIAQLAVASTCLQSCEALGELPA
                     VLPSLLEPLFPAHGGAVFARAAAGEGLGRIAGWGGVDQPDELETGDCLALGQGREHRH
                     GMGAATPACPHLRPALARRNASTLCIPLTAHGETLGLLSLVAVPPAAGAAVEGTTAPA
                     ERLAAMLAQQLGLTLASLRIREVLKEQSIRDPLTGVFNRRYLDVVAPKEIAQALRAGR
                     EMALAMVDIDHFKAFNDRHGHQGGDAALVAVAGFLQASIRLGDWLFRYGGEEFLLLLQ
                     AMDRAALAQQLERLRQGTAAVAARVDGQDLPPVTISIGAAICPDHGQSLEHLLASADR
                     ALYAAKQGGRNRVRLAEPPGPDG"
     gene            complement(5603..6454)
                     /locus_tag="P5558_20100"
     CDS             complement(5603..6454)
                     /locus_tag="P5558_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HRY26682.1"
                     /translation="MPWLDPRGRFSAFKLGVFVLLLVPAALIAWRFASGALGAEPIEA
                     ALHQSGDWAIRLLLVSLAVTPARHLLQWPRLLQVRRMVGVAAFGYAAAHLTLYVADEG
                     FDLGKVAAEIVLRFYLTLGFLAFLVLAAMALTSFDSMIRRLGARRWKLLHRLVYGVAA
                     VAVVHFFMQTKLDATEPWIIAGLLAWLLAARVVIRKGALNGWLADWWPLALGLVASAG
                     TAIGEALYYRALVGVAPSRMLAANLSIEAGIRPAGYVLAICLAAALVIAVRRSTFRAG
                     RLARGTA"
BASE COUNT         1071 a         2253 c         2329 g          960 t
ORIGIN      
        1 ctagcgtatt tttcatggcg tgtgtgtcca gtcggtcgca agcagcagtc attgcagcgt
       61 tcacgttgca gctgctcata tcgtcaggat ttacttcccc ggacgccctt ctctgcccga
      121 ccgctgttag ggggaaacgt cccttgttaa tggttcgccc tgaacaccga ctgaccagcc
      181 cttcctcgcc acggccggga cggctaagtt ccgccggcat cgcgacaagg gaggttggcc
      241 atggcgccag aggaaaagaa cccgctcgcc cggcagcggc tggacggcaa ggtcgtctgc
      301 gtcaccggcg gggcctcggg catcggcgcc gccgccgccc ggctgatggt cgcggccggt
      361 gccagggtcg tgatcctcga ccgcgacgat ggtgccgccg aggccacggc cggggcgatc
      421 ggcggcggct gcgaggccat ggcgctcgat gtcgcgagcg agaccgacat cgaccgggtg
      481 ctggccgaca tcgccggccg ccacgggcgg atcgacgtga tggtcgccaa tgccggcatc
      541 aacatccgcc ggaccgccgt cgactgctcg ctcgacgact ggaacgccgt cctgcaggtc
      601 aacctgacgg gcgtcttcct ctcgtcccgg gccgccgccc ggcacatgcc ggaaaagggc
      661 ggggccgtgg tcgtcaccgc ctcgatcatg agcttctcgg gcggcggcct ctaccccaac
      721 atctcctacg ccacgaccaa gggcgcgctg gtcaacctcg tccgcagcat ggccgtcgag
      781 tgggcgccgc gtaacatccg ggtcaacgcc gtggccccca cctggacgaa gacccggttc
      841 atcaagccgc tcaccgacga tgccgagctg accgcgaagc tggaggcgct gaccccgatg
      901 ggccggctgg ccgagcccga ggaggtcgcc cacgccatgc tcttcctcgc cagcgacgcc
      961 gcctccatgg tcaccggcca caccctcgcc gtggatggcg gcttcttggc gcagtaggcc
     1021 gatcagcttg ccggcattgc gacaagcgag gggacctctt tgttccagca gaggctgcca
     1081 cgcccagcgc tgcaacatta ctccagtcga aggccgcgtc tcggactgaa atccgaccgt
     1141 ttccgcgacg gttgacaccg gctctcgcca gccctcaaca tgcaaaagca acacagctgg
     1201 tagcgcgcag gccgtccaga agggacctcg cccgaaatgc gcagaatagg tatgtggaca
     1261 cgactcggca tcgtggcctc tgtcgtatgg atgctcggat tcggtatcta ttggatagtt
     1321 cagatttctc gttgtaatcc gcagcagtgg tggtcttgtg acttcattgc gggcaccttt
     1381 tggtcgcttc ctgacattct cggcttctgg cggctggggc tatggggcaa tgcgcttttt
     1441 gcatcggttg ctttcccagt cctagtttgg gcggcgctat ggatcgctat cggcgtgacc
     1501 caatggattt gggccggtcg gcagacttca aactagaaca atgccgggtc gaggcgccga
     1561 ttgacatccc gcaccaaaat tggtacaccc attcttgatg agcgcaagcg cgtcccggca
     1621 cgcttctatg caactccgct cgggagcgag cctgttcggg actggctcaa ggggctggcc
     1681 gtcgacgcgc ggcgcaggat cggcttcgat ctcaagcagt tggaatatgg ttggccggtc
     1741 ggcatgccgc tctgtcgccc gctcggacgg agcctttggg agttgcgcag cagcctgccg
     1801 gacggtcgca tcgccagggt gatcttctgc gttcgtgacg gtgagctttt gctgctcgag
     1861 ggcttcatca agaagacgca aaagactccg ccgcaggaga tcgagctggc tttgcaacgg
     1921 atgcgaggaa tgggacgatg accgaaatcg aagacggcag gccaggctcg acgctcgatg
     1981 atttccttgc cgaggaagga ttcctcgagg aggcgaccga gcacgccgtc aagagcgtgc
     2041 ttgcctggca gatcggcgag gcgatgaagg accgcaatct ctccaaggca gccatggcgc
     2101 gcgagatgcg caccagccgc acccagctcg accgcctgct cgaccccgac aacggctcgg
     2161 tcaccctcca caccctgcac cgcgccgccg ccatcctcgg caagcggctc cgggtggacc
     2221 tcgtcgacgg acgccctccc ggcgagcggt gacggcgcgc cgccgtcgcg tcacaagcga
     2281 catccgcctc gtgatgtcgc ggattccgcg acatcatgac gagagatgtc gcggattctg
     2341 cgacacgggg cctcgtgatg tcgcggattc tgcgacacga ttatgacaaa tgtcgcatcg
     2401 ggcgcgaccc tgtcgagcga acgaacccgc ggaatcgacg agcgaaccca ccgattcgac
     2461 gagcgaaccc ggcaaatcga cgagcgaacc caccgattcg acgaacgaac ccgagcctcg
     2521 gccaacgaac ccgaggcctg ccaactcctt gtccggaaag acgattttac ccctgggcag
     2581 cgaccggccc gggaagcggg cgaacccgaa acgcgatctt gccgaacgaa ccgaagcggg
     2641 gcttgcttcc acggcccgat cgtgatagga ataatgtcac gaaccgtcat gttccgccct
     2701 gcccgggacg acgaccggca gcgcctccgg catgatctcg atgtcgagcg gcgttcggcc
     2761 gaccagctcg ccgtcgatgt tggcgcggcg cgggcgggag gtggcgatgg cgatatgcct
     2821 cccctggagc cggaaggcct cgtcgctctc ggcgccttcg atcttgagct tcgccatggc
     2881 ccgcacgagc cggtggacgg ggtcggcgcg cagggcatag aggtcgagca caccgtcggt
     2941 gatggtgccg ctgggcctgg gctcgatgcc gccgccatgg aagcgcccgt tgaccaccgc
     3001 cacgtggatg aaccggtcgc gatggacggt gccgtcgcag gtgatctcga ccctgaacgg
     3061 gtggatccgg cgccaggcgg catagaggac gcgggggtag ttcaggacgc cgagcaggcg
     3121 cttggcggtg ccctggtgca tgcgggcgac ggccgccccg aggccgatgc tgaccgcgtt
     3181 gagcaccggc cggccattga cgctggcgag gtcgatgcgg cgcacgccgc cccgggccac
     3241 gagcctaagc tgctcctccc gggcatccgg caggcggagg ttgcgggcga ggtcgttggc
     3301 cgtgccgagc ggcagcaccg ccaggggctt ctgcaggtcg agcagcgccg gcagggcggc
     3361 ggcgatggag ccgtcgccgc cggcgatggc gatgcggtcg accgtgcggc ccgtcgtgcg
     3421 gatcgtcgcc agcagggcct cgatgccgtc gggctcgacg aagacgacct cgagcccgag
     3481 cccggcgagc ggcgccacga ggtcgctggc cgccgccccg ttgcgcgccc gggggttggc
     3541 gaggagaagg agccggcgcg gcggcaccgc ggcaggggat gggtcggtca tcgcgcgaac
     3601 gttccggggc atcgggtctc gtcgccggtg ctggcgctcg tgtccagaac accgggcaag
     3661 gccgcacgtt ccgcccgccc ggccgatggc gacggcagcc tcacccgtcg ggtccgggcg
     3721 gctcggccag ccgcacccgg ttgcgcccgc cctgcttggc cgcatagagc gcccgatcgg
     3781 ccgacgccag gaggtgctcg aggctctggc cgtggtccgg gcagatggcg gcgccgatcg
     3841 agatggtgac cggcggcagg tcctgcccgt cgacccgggc cgccacggcg gccgtgccct
     3901 gccgcaggcg ctcgagctgc tgggccagcg ccgcgcggtc catggcctgg agcaggagca
     3961 ggaactcctc gccaccgtag cggaagagcc agtcgccgag ccggatgctc gcctgcagga
     4021 agccggccac ggcgaccagc gccgcgtcgc cgccctgatg cccgtgccgg tcgttgaacg
     4081 ccttgaagtg gtcgatatcg accatggcga gtgccatctc ccgcccggcg cgcagcgcct
     4141 gcgcgatctc cttgggtgcc accacgtcga ggtagcggcg attgaagacg ccggtcaggg
     4201 ggtcgcggat cgactgctcc ttcagcacct cgcggatccg caagctggcc agggtgaggc
     4261 cgagctgctg cgccagcatg gcggcgagac gctccgccgg ggctgtcgtg ccctcgaccg
     4321 cagcaccggc ggccggcggg acggcgacga ggctgagcag gccgagggtc tcgccatggg
     4381 cggtgagcgg gatgcagagc gtcgacgcat tgcgccgggc gagcgccggc cggagatgcg
     4441 gacacgccgg cgtcgccgcg cccatgccat gccgatgctc ccgcccctgg ccgagcgcca
     4501 ggcagtcgcc cgtctcgagc tcgtccggct ggtcgacgcc gccccagccg gcgatccgcc
     4561 cgagcccctc gcccgccgcc gcccgggcaa agacggctcc gccatgcgcc gggaaaaggg
     4621 gttcgagcag cgagggcagc acggccggca gctcgccgag cgcctcgcag ctctgcaggc
     4681 aggtgctcgc cacggcgagc tgggcgatct cgttgcgctc ggtgcgcagc tcggcgaggc
     4741 tggcaccgag ctcgtcggca taggccttca acctcctgtt gtaggcgatg cggtcgacga
     4801 cctcgcgccg catgaagccg aagagcgccg cgaccagggc cgccgacacg gtcaggaaca
     4861 cgacgaaggc cgcgaaggcg tggaaccggg cctcctccca gtccgcacgc cgggtgtcga
     4921 gcgtcacggc ctcgacgtcc tgcatctcgc cgaccacgcg gcggagctcg tcgagcgtgc
     4981 cgagctgctg gttgtcccgg atcaggcggc gggccgcctc ggctccctgc tgccggcggg
     5041 cgtcgagggt cctcgacaac tcccggaagc gctcggccac cagcgcccgc acgatggcga
     5101 gccgctgcat ctgcggcgca tccgcctgca gcagcgggcc gattcgtccc aggagctcat
     5161 cggcctgctg cagggccgcg ttgtagggtg cgaggttgct tgcctcgtcg gtcaggatga
     5221 agccgcgatg gcccgactcg gcaccgagca gcacggtcag cagctcggaa aggagctggc
     5281 gggcatcgac gctttcggcc acgtcgcgcg tcgccgccgc cagccggaca tggctgttgt
     5341 agaagaagag gccgttggcc agcagcacgc ccactgtcag ggcgaggacg acgccactgc
     5401 gcagccggaa ggtcccgccc ctgaaccaca tgctcgaccg ggccgcgatg aggtctcgac
     5461 gtgtcgccat cgtcgccacc agtcggcggc ggctgcgcct gccattcagt tcagaagctc
     5521 gggtgccgcg cgccgacact cgccgagtcc gggaggcagg gcaagcccga atcggccgct
     5581 ggcgcgagcc cgcctcgacc ggtcaggccg tgccgcgggc gagccggccg gcccggaacg
     5641 tgctccggcg gacggcgatc acgagggccg cggcgaggca tatggcgagc acgtacccgg
     5701 ccgggcggat accggcctcg atggagaggt tcgccgccag catgcggctc ggggcgacac
     5761 cgacgagcgc gcgatagtag agcgcctcgc cgatcgccgt gccggcagaa gcgacgaggc
     5821 cgagggcgag cggccaccag tcggccagcc agccgttcaa ggcgcccttc cgaatgacga
     5881 cccgggctgc cagcagccag gcgagcagtc cggcgatgat ccagggctcg gtggcgtcga
     5941 gcttggtctg catgaagaaa tgcacgacgg cgacggccgc caccccgtag accagccgat
     6001 gcagcagctt ccagcgccgc gcccccaggc ggcggatcat cgagtcgaac gaggtcagcg
     6061 ccatggcggc gaggacgagg aaggcgagga agcccaatgt gagatagaaa cgaagcacga
     6121 tctcggccgc caccttgccg agatcgaaac cctcgtcggc gacgtagagg gtcaggtgcg
     6181 cggcggcgta gccgaaggcg gcgacgccga ccatgcggcg gacctgcagg agccgcggcc
     6241 actgcagcag atgccgcgcc ggggtgacgg cgagcgagac cagcagcagg cgaatggccc
     6301 agtcgccgga ctggtgcagc gccgcctcga tcggctcggc gccgagggca ccgctggcga
     6361 aacgccaggc gatgagcgcc gccgggacga ggaggagaac gaaaacgccg agcttgaagg
     6421 ccgagaagcg tcctctgggg tcgagccagg gcatggggtc gctgggtccg atcctgctat
     6481 ccgtgggttg cagggtgtca caacataggt cgtggcgtca cgggcggccc tgcacacgcc
     6541 ggatcaagtt tcggcgaagg ccgcgttgct cgcgaggccg cgcggtcgtc ggctacgcct
     6601 tgccataccc ccc
//
LOCUS       DAOTFI010000252         6612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13777_length_6612_cov_6.917950, whole genome shotgun sequence.
ACCESSION   DAOTFI010000252 DAOTFI010000000
VERSION     DAOTFI010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13777_length_6612_cov_6.917950"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="P5558_20105"
     CDS             <1..823
                     /locus_tag="P5558_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY26683.1"
                     /translation="HAQTEKLIGSGRIDHINFTGSVGGGRAIERAAAGTFATLGLELG
                     GKDPAYVMADAKLDHAIENLVDGAFYNSGQCCCGIERIYVDAAVYDDFVAGVAELTGK
                     YVVGSPLDPATTLGPMARGKFADLVREQVAEARRKGATALVNMAVEGDRPGSPYIAPE
                     VLVGVDHQMAVMREESFGPVVGIMPVAGDDAAIELMNDSAYGLTASLWTRDRDAAQAL
                     GGRLRTGTVFMNRCDYLDPALVWTGVKDTGRGQSLSVLGYQALTRPKSYHLRSVP"
     gene            820..1995
                     /locus_tag="P5558_20110"
     CDS             820..1995
                     /locus_tag="P5558_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRY26684.1"
                     /translation="MSASPSVNWGYPTTVRFGAGRIAELPRACAAAGITRPLFVTDAG
                     LKELPITKAARGLLADAGFFAEVKANPVDDNVEAGVRAFRDGGHDGVVAFGGGSALDV
                     GKCVAFMAGQTRPIWDFEDVDDWWKRADTAGIAPVVAVPTTAGTGSEVGRAGVITNSA
                     THTKKVIFHPRMLPAVVISDPELTLGVPPTITAGTGMDALAHCLEAYFSPMFHPMSEG
                     IAVEGARLVKEALPRAVADGQDLEARAWLLAAASMGAVAFQKGLGAIHSVSHPVGAIY
                     DTHHGLTNAVFMPYVLAFNRPAIESKAARLAAWLGLARQDLDGLIDWVLALRDAIGVP
                     HTLAGLGVDGARFREMAEMAVVDPTASGNPVPLDVDAALAIFEAAASGELPSSTCAL"
     gene            2169..2564
                     /locus_tag="P5558_20115"
     CDS             2169..2564
                     /locus_tag="P5558_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26685.1"
                     /translation="MAAAVPAPGEAGFLGLLDGKDFIVEYGPVGEPSLGQDVLSFESG
                     RFRSRGCSDLGFVPAPYWLRLEGDAIRFRAAMESPEHGYIDFTGEVVGEHIEAGSIWT
                     RERWYRTVVLESWYRGALAAPGQALPEKS"
     gene            2561..3259
                     /locus_tag="P5558_20120"
     CDS             2561..3259
                     /locus_tag="P5558_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26686.1"
                     /translation="MSGPPSGAMGEGPLPGWPLRRILRLLALVVLYGAIVLGGTYAGD
                     WIVNLLGMRLTPTSEPTVHLVIMLSLAAYVVLMALPFVPGVEVGLALIFMLGAEIVPV
                     VYLGTVLALVIAFAVGRLVPERLLAAGFAALGLGRAARLVERLQPLAVEARVAALAAA
                     SPAGIATLLVRRRHWAIALLLNVPGNGLIGGGGGIAMAVGMSRLVSLPGFLVTIALGT
                     APVPVAVLVMEALR"
     gene            complement(3408..5342)
                     /locus_tag="P5558_20125"
     CDS             complement(3408..5342)
                     /locus_tag="P5558_20125"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HRY26687.1"
                     /translation="MTTHKERVLRVLRGETVDRLPYVPRIDLWHLANSVAGTLPPEHA
                     GRTQHEISRAEGWAIHHKYVYGAFGEHPDDALLHRGIGIFRTRDTVLDFVLPRDVGVE
                     VEHDGPVMRVTYHTPAGVVSHRLRYDESMQRLGISIPLHEDHLIKSVADYPAVWHLFE
                     HMQVVPNHERFSRWAETEIGEDGVPVAAHFHGASPMQQIQRDLIDATAFFIHYKDHQR
                     ELRELAARMEPLFERILEICCASPAPVVNWGSNFDDMLTYPPYFAREIQPWIRRASAA
                     LADAGKILMCHTDGENRGLMELIRDSGMHVAESICPAPMTQVGLAEYYREWAGKLTLF
                     GGVPSTLLLPETSEAAFDAYVDELFRAIAPGSRFVVGIADQVPPNAVFARLRRLGQRI
                     AEEGRLPLEAGGFRPAMPPPAQVAAPSARGAADDEERFYEVQDDVHAGDHLAIRQRVL
                     ALVEDGVPAQEILERGLIAAMDEIGQAFASGEAFIPEVLLSARAMNEAVDALGPHLAT
                     GAERHRGRVLVGTVAGDVHDIGKNMVVTMLKGVGFEVQDLGVNVPRARFVAAVAESKP
                     DILGLSALLTTTMMEMRQVIASLEAAGLRDTCRVIVGGAPLNAGFANEIGADGFAEDA
                     VSAVALAKQLVAERRTPPAA"
     gene            5664..>6612
                     /locus_tag="P5558_20130"
     CDS             5664..>6612
                     /locus_tag="P5558_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY26688.1"
                     /translation="MAKDIGHLIGGKRAAGTSGAFGPIFDPATGEESGRVAMGGPDEV
                     AAAVEAARAAQPAWADTSIVRRARVMFRFKELIEKNMDELAKLVTSEHGKTIDDAKGS
                     ITRGLEVVEFACGIPQLLKGEFSHNVGTGVDLLSVRHPVGIAAGITPFNFPAMIPLWM
                     APLAIACGNAFILKPSEKDPSCPMLLAELLQEAGAPAGIIQVVNGGKDAVNAILAHPG
                     IAAVSFVGATATAEHIYRTGTMAGKRVQALGGAKNHMVIMPDADLDMAVDALMGAAYG
                     SAGERCMAVSVAVPVGDATADALIEKLAPKVRQLKIGPGT"
BASE COUNT          924 a         2341 c         2355 g          992 t
ORIGIN      
        1 gcatgcccag accgagaaac tgatcggctc cggccgcatc gaccacatca acttcaccgg
       61 ctcggttggc ggcggccgcg ccatcgagcg cgccgcggcc ggcaccttcg ccaccctcgg
      121 cctcgagctc ggcggcaagg acccggccta tgtcatggcc gatgcgaagc tcgaccacgc
      181 catcgagaat ctcgtcgacg gcgccttcta caattccggc cagtgctgct gcggcatcga
      241 gcggatctac gtcgacgccg cggtctatga tgacttcgtc gccggcgttg ccgagctcac
      301 cgggaagtac gtcgtcggca gcccgctcga cccggccacc accctcggcc cgatggcgcg
      361 gggcaagttc gccgatctcg tccgcgaaca ggtcgcagaa gccaggcgca agggcgcgac
      421 cgcgctcgtc aacatggcgg tcgagggcga tcgccccggc tcgccctata tcgccccgga
      481 agtgctggtc ggggtcgacc accagatggc ggtgatgcgc gaggagagct tcgggccggt
      541 ggtcggcatc atgcccgtcg ccggcgacga cgctgccatc gagctgatga acgacagcgc
      601 ctacggcctc accgcctcgc tctggacccg cgaccgcgac gccgcgcagg ccctgggcgg
      661 gcggctgcgg accggcacgg tcttcatgaa ccgctgcgac tatctggacc cggcgctggt
      721 ctggaccggc gtcaaggaca ccggccgcgg ccagtcgcta tcggtgctcg gctaccaggc
      781 gctgacccgc cccaagtcct accatctcag gagtgtgcct tgagcgcttc gccatccgtc
      841 aactggggct atccgaccac cgtccgcttc ggcgccgggc ggatcgccga gctgccgcgc
      901 gcctgcgccg cggccggcat cacccgtccg ctcttcgtca ccgacgccgg gctgaaggag
      961 ctgccgatca ccaaggcggc gcgcggcctc ctggccgacg ccggcttctt cgccgaggtc
     1021 aaggcgaacc cggtcgacga caatgtcgag gccggcgtcc gggccttccg cgacggcggc
     1081 catgacgggg tcgtggcctt cggcggcggc agcgccctcg atgtcggcaa gtgcgtcgcc
     1141 ttcatggccg ggcagacccg gccgatctgg gatttcgagg atgtcgacga ctggtggaag
     1201 cgggccgaca cggccggcat cgccccggtc gtcgccgtgc cgaccacggc cggcaccggc
     1261 tccgaggtgg ggcgcgccgg ggtcatcacc aactcggcga cccacaccaa gaaggtgatc
     1321 ttccacccga ggatgctgcc cgccgtggtg atcagcgacc ccgagctgac gctgggcgtg
     1381 ccgccgacga tcaccgccgg caccgggatg gacgccctgg cgcactgcct cgaggcctac
     1441 ttctcaccca tgttccatcc gatgtcggaa ggcatcgcgg tcgagggggc gaggctcgtc
     1501 aaggaggcgc tgccgcgcgc cgtggccgat ggccaggacc tcgaagcgcg cgcctggctg
     1561 ctcgcggcgg cctcaatggg tgccgtcgcc ttccagaagg ggctgggtgc gatccattcg
     1621 gtctcgcacc cggtgggtgc gatctacgac acccatcacg gcctcaccaa cgccgtcttc
     1681 atgccctatg tgctggcctt caaccgcccc gccatcgaga gcaaggccgc ccgcctcgcc
     1741 gcctggctcg gcctcgcccg gcaggacctg gacgggctga tcgactgggt gctcgccctg
     1801 cgcgacgcca tcggcgtgcc gcacacgctc gcggggctcg gcgtggacgg cgcccgcttt
     1861 cgggagatgg ccgagatggc ggtggtcgat ccgaccgcaa gcggcaatcc ggtgccgctc
     1921 gacgtcgatg cggcgctggc gatcttcgag gccgcggcga gcggcgagct gccatccagc
     1981 acttgcgccc tttagggatc ggagtcagat tcgcgatgcc ggcgccgcgt cagccggcgt
     2041 gcagcgcgtc gtggaaggcg gcagggacgt tgcggaaggc tcgtgggctg aagcgtcgac
     2101 ggcgtgaaat ccttctctgg agcctcggca cggctatggc agcctcgggc acagcgttgc
     2161 cggagggctt ggcggcggcc gtgcccgcgc ccggcgaagc gggtttcctc ggactgctcg
     2221 acggcaagga cttcatcgtc gaatatgggc cggtgggcga gccatcgctt gggcaggacg
     2281 tgctgagctt cgagagcggg cgcttccggt ctcgcggctg cagcgatctg ggcttcgtgc
     2341 cggcgccgta ctggctgcgg ctcgaaggtg acgcgatacg gttccgggcg gccatggaga
     2401 gccccgagca cggctatatc gacttcaccg gcgaggtcgt cggcgagcac atcgaggccg
     2461 gctcgatctg gacacgcgag cggtggtacc ggacggtcgt gctcgaatct tggtatcggg
     2521 gagcgctcgc cgcacccggc caggcgttgc cggagaagtc atgagcggcc cgccttccgg
     2581 cgccatgggc gaggggccgc tcccgggctg gccgctgcgg cggattctgc ggctcttggc
     2641 gctcgtcgtg ctctacggtg cgatcgtgct cggcggcacc tatgccggcg actggatcgt
     2701 caatctgctc ggcatgcgcc tcaccccgac ctccgagccc accgttcacc tggtcatcat
     2761 gctcagcctc gccgcctatg tggtgctgat ggcgctgccc ttcgtgccgg gcgtcgaggt
     2821 cggcctggcg ctgatcttca tgctgggggc ggagatcgtg cccgtggtct atctcggcac
     2881 ggtgctggcg ctggtgatcg cctttgctgt cggccgcctc gtgccggagc ggctgctggc
     2941 cgccggcttc gccgcgctcg gcctcggccg cgccgcccgg ctggtcgagc ggctgcagcc
     3001 gctggccgtc gaggcgcgcg tggcggcgct ggccgccgct tccccggctg gcatcgcgac
     3061 gctgctcgtg cgccggcggc actgggcaat cgccctcctg ctcaacgtgc cgggcaacgg
     3121 gctcatcggc ggcggtggcg gcatcgccat ggccgtcggc atgagccggc tggtctcgtt
     3181 gccgggcttc ctcgtcacca tcgccctcgg caccgccccg gtgccggtcg cggtgctggt
     3241 catggaggcg ttgcggtagg tgccctgccc cgagggggcg aggggagacg ggagcagcag
     3301 ggccgaggcc cgcccggatg ccagggcgcg cttcgaggcg tcgtcccgtt gccccgacaa
     3361 cggcggcacc ggggtccgcc ggccccggtc gagaaccctc ccgaggctca tgccgcgggc
     3421 ggggtccggc gctcggcgac cagctgcttc gccagggcca ccgcgctcac ggcatcctcg
     3481 gcgaagccgt cggcgccgat ctcattggcg aacccggcgt tgagcggggc gccgccgacg
     3541 atcacccgac aggtatcgcg aaggcccgcc gcctcgaggc tggcgatgac ctggcgcatc
     3601 tccatcatgg tcgtggtcaa gagggccgag aggccgagaa tgtccggctt gctctcggcc
     3661 accgccgcga cgaacctcgc ccgcgggacg ttgacgccga ggtcctggac ctcgaagccg
     3721 acgcccttga gcatcgtcac caccatgttc ttgccgatgt cgtggacgtc gccggccacc
     3781 gtgccgacaa gaacccggcc gcggtgtcgc tcggcgccgg tggcgagatg aggtccgagg
     3841 gcgtccaccg cctcgttcat ggcgcgcgcc gacaggagca cctcggggat gaaggcctcg
     3901 ccgctggcga aggcttgccc gatctcgtcc atcgcggcga tcaggccgcg ctccaggatc
     3961 tcctgggccg gcaccccgtc ctcgaccagc gccagcacgc gctggcggat ggccaggtga
     4021 tcgccggcgt ggacgtcgtc ctggacctcg tagaagcgct cctcgtcgtc cgccgcgccc
     4081 ctcgcgctcg gcgccgccac ctgggccggg ggcggcatgg ccggccggaa gccgccggcc
     4141 tcgagcggca gccgcccttc ctcggcgatc cgctggccga gccgccgcag gcgcgcgaac
     4201 accgcgttcg gcggcacctg gtcggcaatc ccgacgacga accggctgcc cggcgcgatc
     4261 gcccggaaaa gctcgtcgac ataggcgtcg aacgcggcct cgctggtctc cggcaggagc
     4321 agggtcgagg gcacgccgcc gaagagcgtc agcttcccgg cccactcccg gtagtactcg
     4381 gcgaggccga cctgcgtcat cggcgccggg cagatcgatt cggcgacatg catcccggaa
     4441 tcgcggatca gctccatgag cccgcgattc tcgccgtcgg tgtggcacat caggatcttt
     4501 ccggcatcgg cgagcgccgc cgaggcacgg cggatccagg gctggatctc ccgggcgaag
     4561 tagggcgggt aggtcagcat gtcgtcgaag ttggagcccc agttgacgac cggcgccggc
     4621 gaggcgcagc agatctcgag gatgcgctcg aagagcggct ccatccgtgc cgcgagctcg
     4681 cgcagctcgc gctgatggtc cttgtagtgg atgaagaagg cggtggcgtc gatcaggtcg
     4741 cgctggatct gctgcatcgg cgacgcgcca tggaaatgcg ccgccaccgg caccccgtcc
     4801 tcgccgatct ccgtctcggc ccagcggctg aagcgctcgt ggttgggcac cacctgcatg
     4861 tgctcgaaca ggtgccagac ggccggatag tcggcgacgc tcttgatcag gtggtcctcg
     4921 tgcagcggga tcgagatgcc gagccgctgc atcgactcgt cgtagcgcag gcggtggctg
     4981 accaccccgg ccggcgtgtg gtaggtgacc cgcatgaccg ggccgtcatg ctccacctcg
     5041 accccgacat cccgcggcag cacgaagtcg aggacggtgt cgcgggtccg gaagatgccg
     5101 atgccgcgat gcagcagggc gtcgtccggg tgctcgccga aggcgccgta gacgtatttg
     5161 tggtggatcg cccagccttc ggcgcgcgag atctcgtgct gtgtgcggcc ggcgtgctcc
     5221 ggcggcagcg tcccggcgac cgagttggcg aggtgccaga gatcgatccg tggcacgtag
     5281 ggcagtcgat ccaccgtctc gccacgcaat acccgaagaa cccgctcctt gtgcgtcgtc
     5341 acgtcgctct cccccgcatt cgccagccgg ccgccccggc ggggcggcag tcacgggtcg
     5401 agggaccccg cgcccgcgat ccccatgcga gttgaatggt aacgttgcca gtgtcgaggc
     5461 accttgacga cggtcaactc cgttgccccc aagaatgggc gcagccgtct cccgcccggc
     5521 aggtctcgca gccggggtgc ccgcgacatg ccgggcctgc acgaccccgc gaaggcgctc
     5581 gcggctgtac gcggcggccg cgaagcgcta cttgcggatg ccagacaggg tccgtcgaca
     5641 ggcggcgacg cagggagaga gtgatggcga aggacatcgg gcatctgatc ggcggcaagc
     5701 gggcagcggg caccagcggc gccttcgggc cgatcttcga tccggcgacg ggcgaggaga
     5761 gcggccgggt ggcgatgggc ggccccgacg aggtggcggc cgccgtcgag gcggccaggg
     5821 cggcgcagcc ggcctgggcc gatacctcga tcgtccgccg ggcgcgggtg atgttccgct
     5881 tcaaggagct gatcgagaag aacatggacg agctggcgaa gctcgtcacg tccgagcacg
     5941 gcaagaccat cgacgacgcc aagggctcga tcacccgcgg gctcgaggtg gtcgagttcg
     6001 cctgcggcat cccgcagctc ctcaagggcg agttcagcca caatgtcggc accggcgtgg
     6061 acctcctctc ggtccgtcac ccggtcggca tcgccgccgg catcacgccc ttcaacttcc
     6121 cggcgatgat cccgctctgg atggcgccgc tcgccatcgc ctgcggcaac gccttcatcc
     6181 tcaagccgtc ggagaaggac ccgtcctgcc cgatgctgct cgccgagctc ctgcaggagg
     6241 cgggcgcacc cgccggcatc atccaggtgg tcaacggcgg caaggacgcg gtgaacgcca
     6301 tcctggcgca tccgggcatc gcggcggtga gcttcgtcgg ggccacggcc acggccgagc
     6361 acatctaccg gaccggcacg atggccggca agcgcgtgca ggcgctgggc ggggccaaga
     6421 accacatggt catcatgccc gacgccgatc tcgacatggc ggtcgatgcg ctgatgggcg
     6481 ccgcctacgg ctcggccggc gagcgctgca tggcggtctc ggtcgcggtg ccggtcggcg
     6541 acgccacggc cgacgccctg atcgagaagc tcgccccgaa ggtgcgccag ctcaagatcg
     6601 gcccgggcac cg
//
LOCUS       DAOTFI010000253         6591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13833_length_6591_cov_4.932987, whole genome shotgun sequence.
ACCESSION   DAOTFI010000253 DAOTFI010000000
VERSION     DAOTFI010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13833_length_6591_cov_4.932987"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="P5558_20135"
     CDS             complement(<1..468)
                     /locus_tag="P5558_20135"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HRY26689.1"
                     /translation="MILFGASETGLELRRQADGSTRLAGRFPYNRLAVLSDGGRNGRP
                     RKEQFAPRAFGFRVDQPSEEIHLLVGHDFAKPLASRGAGTLRLTDSDEALTFEATISQ
                     AIAETSFGRDALALIAAGLAVGLSPGFRLPPERAVPRDQAERIEEEPMRPEAGM"
     gene            complement(474..1763)
                     /locus_tag="P5558_20140"
     CDS             complement(474..1763)
                     /locus_tag="P5558_20140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRY26690.1"
                     /translation="MLESVKIARRQSEIRQELATLAAKAEPSEDETRSMEKLDGEYRT
                     NETRYRAALVAEDVERRDAKGELETRGDRQWSDLVQRFELRQVALALDEGRKLDGATA
                     EVVEELRSSGGYRGYPIPLESLVLERRAGETIASGVPNPIATMPIIDRLFADSAALRM
                     GAQMISIPQGEVEFPVATSGATVAWQQTETGNVGGPSAYATTDRPLKPDNTLGVQMKL
                     TRKSLKQSGASLEQAVRRDMAGAMQAAIDKAIFLGAGASGEPSGVIAKASTYGITETS
                     IGAEPSWAIFRKAVVRFMLSNAVSAPTQVRAMFRPEVWDDLDAMRAGDGYPIWEMDRV
                     VAAMGAGNITLSSNALAAPAGDPLVTSALLTTTAGGVAPIFVGLWGAIDLIRDPYSDA
                     QSGGLRVTALATVDVTISRSAQLEVLTNIKRHVLAGG"
     gene            complement(2085..3599)
                     /locus_tag="P5558_20145"
     CDS             complement(2085..3599)
                     /locus_tag="P5558_20145"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HRY26691.1"
                     /translation="MTAAKRTLAFLAGLRVPEGPRAGKPLKLAPFQRSFVRGALKPST
                     SVAALSVARGNAKTALAAGIALAELLGHLERQPRREILIAARSRDQAGVALALAWGLA
                     QSLDDDTREGLQLRRAPRAEIEFHGDGGGHVLRAIAADGKSILGGAPTLAILDERGHW
                     PGGRGDELEGAILSGLGKRGGKALVISTSAADDAHPFSRWLDAPPPGAFVQEHRPAPG
                     LPPDDLESLLLANPGARHGIGSSPAWLKAAAERAIARGGSALLGFRLYNRNERVSGEA
                     RDLLLTVDEWMACEADPLPPRSGPVVIGCDLGGSASMSAAAYFWPETHRLEVKAWFPG
                     APSLLHRGQADGVGDRYCVMQDRGELATLGERTVPVAAWFEAIMARVQGQHVAALCAD
                     RYKSAELGEAMDRAKVRAPIVWRGMGWRDGAEDVERFRRAVYDGKVLAASSLLMRSAL
                     GDAVCLKDPAGNSKLAKGRSMGRIDAAAAAVLAVAEGARMTARPAPRPAKLIFI"
     gene            complement(3681..4574)
                     /locus_tag="P5558_20150"
     CDS             complement(3681..4574)
                     /locus_tag="P5558_20150"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY26692.1"
                     /translation="MAAEIIPLKTRPGKALLDKMRFVELLTARRDLSRLELRVALRLV
                     SLTNMKRGFAYPPYEQLVGELGASRSKVAAALSRLRELGLFTVRPGGGRRPGGGPGRA
                     NEYTPAWDRIPADCPFTAAAQTVPHAGRFTDQDGHGGTVPFSRGTVPFSRGTVPYGGT
                     PSEESYIRSVPTAPTGKPSARSVPHRHEGQDDEANHIEDLKALWIEPLPGGALQRAER
                     AIKAGTVQRLAQRDRLRLHDRLESISDEYERQDGHPIGGHALRLAMLVEDDIEHDDDG
                     VPVWPEGWHDQRWNGEGDDDE"
     gene            complement(4716..4931)
                     /locus_tag="P5558_20155"
     CDS             complement(4716..4931)
                     /locus_tag="P5558_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26693.1"
                     /translation="MSELLSGYLDRAALAKELNVCQRTIARYESQPNGLPVTVIGGRR
                     LYKMTSVMAWLEARERRPNPTRARRAA"
     gene            complement(5024..5500)
                     /locus_tag="P5558_20160"
     CDS             complement(5024..5500)
                     /locus_tag="P5558_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26694.1"
                     /translation="MTLQDQLDQMAAWFTGFRSRDKALAGDRTSLLERLRDPGVELIQ
                     PERDLLAAIGEGKVKRPRGPRRDDDLRAKKMQAAGFFILLTEAIGLLHKEAEGRLADF
                     YGVADTTVHDWIGDEKKRIGLAAWQAKIASAPADFALDRPMYMENERLAELLKSAE"
     gene            complement(5497..>6591)
                     /locus_tag="P5558_20165"
     CDS             complement(5497..>6591)
                     /locus_tag="P5558_20165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY26695.1"
                     /translation="RFTIGRWPDWKTTSARAEAKRLRQAVDRGEDPMAAIHAARSETK
                     VRKGAETFAEAVADYVTREQKGRRQNATAGEVQKILLRACAGWLSRPVAEIDGRDIRA
                     LVEAIRDGDDDAAPRPYLAVKTHALLSGFFGWCAEPGVEKVKASPMIGLRKPWKGEEA
                     RDRVFNDDELRALWQASDEISGTAGAYLKVLLLTGKRKIALAAMRWREIDGSGFWTPP
                     ADNRLRRRTKRLHAVPLPRLALAAIEPLRPPADATDASPFVFRGRVRGTHMDPGTPFQ
                     RTVQAASGVDDFFPHACRHTVETRMAELGIAPHVRDLILDHAPARGSGSGYDHHHYGP
                     EMRDALERWAGHIERLVTPGEVVAFPGRGR"
BASE COUNT          951 a         2323 c         2179 g         1138 t
ORIGIN      
        1 cattccggcc tctggccgca tgggctcttc ctcgatccgc tcggcctggt ccctcggcac
       61 ggcccgctcg ggcggcaacc tgaagcccgg gctcaacccg acagcgagtc cggcagcgat
      121 cagcgccagg gcatcccggc cgaagctcgt ctccgcaatc gcctggctga tcgtcgcctc
      181 gaacgtcagg gcctcatcgc tgtccgtgag ccgcagggtg ccggcgccgc ggctggccaa
      241 gggcttggcg aagtcgtgtc cgaccagcag atggatttcc tcgctcggct gatcgacccg
      301 aaagccgaag gcgcgcggcg cgaactgctc tttgcgcggc ctgccgttcc gcccgccgtc
      361 gctgagcacg gccagccggt tgtacgggaa ccggccggcc agccgagtcg agccgtcggc
      421 ctggcgccgc agctcgagcc cggtttcgct ggccccgaag aggatcatgg gcctcagccg
      481 ccggcgagaa cgtggcgctt gatgttcgtc aaaacctcga gctgcgccga gcgggaaatc
      541 gtcacgtcca cggtcgccag ggcggtgacc ctgagcccgc cggactgggc atcgctgtag
      601 ggatcgcgga tcaaatcgat cgcgccccac agccccacga agatcggcgc cacgccgccg
      661 gcagtcgtcg tgagcagcgc cgaagtcacc aagggatcgc ccgccggtgc ggcgagcgcg
      721 ttgctgctca gagtgatgtt gcccgcgccc atcgcggcaa caactcgatc catttcccaa
      781 atcggatacc cgtcgccggc ccgcatcgcg tcgagatcat cccaaacttc cggcctgaac
      841 attgctcgga cttgcgtcgg tgccgaaact gcattgctca acatgaaccg gacaacggcc
      901 ttgcggaaga tcgcccacga gggctcggcg ccgatgctcg tctcggtgat cccgtaggtg
      961 ctggccttgg cgatgacgcc gctcggctcg cccgaggcac cagcgccgag gaagatcgcc
     1021 ttgtcgatcg ccgcctgcat cgcgccggcc atgtcccggc gaaccgcttg ctcgaggctg
     1081 gcgccggact gcttcaacga cttgcgggtg agcttcatct gcacgccgag ggtgttgtcc
     1141 ggcttcagcg gccgatccgt cgtcgcgtag gcgctcggcc cgccgacatt gccggtttcc
     1201 gtctgctgcc aggcgacggt ggcaccgctg gtcgcgaccg ggaactcgac ctcgccctgc
     1261 gggatgctga tcatctgcgc gcccatccgc agggccgcgg agtcggcgaa caaacgatcg
     1321 atgatcggca tggtcgcgat cgggttcggc acgccgctcg cgatggtctc gcccgcccgg
     1381 cgctcgagga ccaggctctc gagcgggatc ggatagcctc ggtaaccgcc cgagctgcgc
     1441 agttcctcga cgacttccgc cgtggccccg tcgagcttcc tgccctcgtc gagggcgagg
     1501 gccacttggc gcagctcgaa ccgctgcacc aggtccgacc actggcgatc gccgcgcgtc
     1561 tcgagctcgc ccttggcgtc gcggcgctcg acgtcttcgg cgaccaaggc ggcgcggtag
     1621 cgcgtctcgt tcgtgcggta ctcgccgtcg agcttttcca tagagcgggt ttcgtcctcg
     1681 gacggctcgg ccttcgcggc gagggtcgcg agttcctgcc tgatttcgga ctggcggcgg
     1741 gcgatcttga cgctttcgag catgtggact ccggtcggtc tacttgcggc cggacagttc
     1801 cttgaccgcg gacaaccacg ctcttgcgcc ttcgcctctt tccgggcgtc cgatttctcg
     1861 acgagtcttc ctgctgtgac agctcggaca cagcgcctgc aggttgtcga gcacccatcg
     1921 cagctcggga tgcgtccgct caggcaggac gtgatccacc tcgaggcggc ccctggcacc
     1981 gcactgcacg cagcgaaatc cgtcgcgccg cagggcaagc agccgcagcc ccttccatcg
     2041 cctcgagctg gtcgtcgcgc ggctgtagcg gtgccagcgg tctttcaaat gaagatcaac
     2101 ttcgccggcc gcggcgcggg cctcgccgtc atgcgcgcgc cctcggccac ggccaggacg
     2161 gcagcggcgg ccgcgtcgat gcggcccatc gagcggccct tggcgagctt gctgttcccc
     2221 gctggatcct tcaggcatac cgcgtcgccc aaggctgagc gcatcagcag cgaagacgcg
     2281 gccagcacct tgccgtcgta gaccgcgcgg cggaatcgct cgacgtcctc ggcgccgtcc
     2341 cgccagccca tgccgcgcca cacgatcggc gcccgcacct tcgccctgtc catggcttcg
     2401 cccagctcgg cggatttgta gcggtcagcg cagagcgcgg cgacgtgctg gccctgcacc
     2461 cgggccatga tggcctcgaa ccaggcggcg actggaaccg tcctctcgcc gagcgtcgcc
     2521 agctcgccgc gatcctgcat cacgcaatag cgatcgccga ccccatcggc ctgcccgcgg
     2581 tgcaagaggc tcggcgcgcc cgggaaccag gccttcacct cgaggcggtg cgtctctggc
     2641 cagaaatagg ccgcggcgct catgctggcg ctgccgccca agtcgcagcc gatcaccacc
     2701 ggccccgatc gcggcggcag cggatcggcc tcgcacgcca tccactcgtc tacggtcaag
     2761 agcaagtcgc gggcctcgcc gctcacccgc tcgttgcgat tgtacagcct gaagcccagc
     2821 agcgcggaac cgccccgtgc gatcgcccgc tcggccgccg ccttcagcca tgccgggctc
     2881 gacccgatgc catgccgggc gcccggattc gccagcagca gggactcaag atcgtccggc
     2941 ggcaggcctg gcgctggccg gtgttcctgg acgaaggcgc cgggcggcgg tgcatcgagc
     3001 cagcgcgaga agggatgggc atcgtcggcc gcgctggtcg agattaccaa ggccttgccg
     3061 ccccttttgc ccaggcccga caggatcgcg ccctcgagtt catcaccccg gccgcctggc
     3121 cagtggccgc gctcgtccag gatcgcgagc gttggtgcac cgccgaggat gctttttccg
     3181 tcggcggcga ttgcccgcag gacgtggccc ccaccgtcgc cgtgaaattc tatctctgcc
     3241 ctcggcgccc ggcgcagttg cagtccttcc cgcgtgtcgt cgtcgaggct ctgggccagc
     3301 ccccaggcca gggcgagcgc caccccggcc tggtcccgac tcctggctgc gatcaggatt
     3361 tcccttcgag gctggcgctc gaggtggcca agcaactcgg ccagggcgat gccggcggcg
     3421 agggcggtct tggcgttgcc tctggccacg ctgagggcgg caaccgaggt gctgggcttg
     3481 agcgcgcctc ggacgaagga tcgctggaag ggtgcgagct tgagcggctt accggcccga
     3541 ggcccctcag gaacccgcag gccggccagg aaggcgaggg tccgttttgc cgccgtcatc
     3601 ggccgcctcg gcgagcgaga agggaaaagc cccccctgcg tgctgcgccc ccccagacaa
     3661 tcgcggcatt gggaccaatc tcattcatcg tcgtcgcctt cgccgttcca gcgctggtca
     3721 tgccagccct ctggccacac cggcacgccg tcgtcgtcgt gctcgatgtc gtcttcaacg
     3781 agcatagcga ggcgcagggc gtgcccccca atcggatgac cgtcttgtcg ttcgtattcg
     3841 tcggaaatcg attccagtcg atcgtgcagc ctcagccgat ccctctgggc aagccgctgg
     3901 acggtgccgg ccttgatggc tcgctcggct cgttgaagcg cgccaccagg caagggctca
     3961 atccacaatg ccttgaggtc ttcgatgtgg ttggcttcgt cgtcctgtcc ttcatgcctg
     4021 tgaggcacag agcgcgccga cggcttgccc gttggcgctg tgggtacaga acggatgtaa
     4081 gattcctcgg atggcgtccc cccgtagggg acggttccac gcgaaaaggg gacggttccg
     4141 cgcgaaaagg ggacggttcc gccatgccca tcctggtcgg taaaccgtcc cgcatggggg
     4201 actgtttggg cggctgccgt gaaggggcaa tcagccggta tccgatccca tgccggggtg
     4261 tactcgttcg ccctgccggg tccgccaccg ggccggcgtc cgccaccggg ccggacggtg
     4321 aacagcccga gctctctcag gcgcgagagc gcggcagcaa ccttgctccg agacgcgccg
     4381 agctcgccca caagctgttc gtagggcggg taggcgaagc cgcgcttcat gttggtgagc
     4441 gagacgaggc gaagggccac gcgaagctcg aggcggctca gatcccggcg agcggtgagc
     4501 aactcgacga agcgcatttt gtccagcagc gccttgcccg ggcgcgtctt cagggggatg
     4561 atttcagcgg ccatgcgtgc gggctacttc tcgcaggcgc cgactcgtgg tagatcaccg
     4621 gggtcaggtg gcgatccgcg tgaggtggcg cttctggtgg gaattcccga acgggcggcg
     4681 tctcgcgaac gtcgcccgtt tcgcgttcac gggcgtcagg cagcgcgcct ggccctggtc
     4741 gggttgggcc ggcgctcgcg agcctcgagc caggccatga ccgaggtcat cttgtagagg
     4801 cgccttccgc cgatgacggt caccggtagc ccgttcggct gagactcata tcttgcgatg
     4861 gtgcgttggc agacgttcag ctctttggcg agcgctgccc ggtcgaggta tccagaaagc
     4921 agttcactca ttggtgcggt ccagttctgt cgtggaagcc gctatccatc tgtcatgctc
     4981 gccggctgtt gtcgtccaga atcagtgcgg agctattcgc gctttactcc gcacttttca
     5041 gcagctcggc cagccgctcg ttttccatgt acatcggtcg gtcgagggcg aagtcggccg
     5101 gcgccgacgc aatcttggcc tgccatgcgg ccagaccgat ccgcttcttc tcgtccccga
     5161 tccaatcgtg aaccgtcgtg tcggccaccc cgtagaagtc ggccagcctg ccctctgctt
     5221 ccttgtggag cagtccgatc gcttcggtca ggaggatgaa gaagccggca gcctgcattt
     5281 tcttcgcccg caggtcgtcg tcccgccgcg ggcccctcgg ccgcttcacc tttccctcgc
     5341 cgatggcggc aaggaggtcg cgctccggct gaatcagctc gacgcccggg tcgcgcaagc
     5401 gctcgagcag cgaggtgcgg tcaccggcga gcgccttgtc gcggctgcga aagccagtga
     5461 accacgccgc catctggtcg agctggtcct ggagcgtcac cggccgcggc ccggaaaggc
     5521 aactacttcg cccggtgtca ccagccgctc gatgtgcccg gcccatcgct ccagggcgtc
     5581 gcgcatctcc ggcccatagt ggtggtggtc gtatccgctg ccggagccgc gcgccggcgc
     5641 atggtcgagg atgaggtcgc ggacgtgagg cgcgatgccc agctcggcca tgcgcgtctc
     5701 gacggtgtgg cggcaggcat gagggaagaa gtcgtctacg ccagacgcag cctggacggt
     5761 cctctggaac ggcgtgccgg gatccatgtg agtgccccgc acccggccgc ggaagacgaa
     5821 gggactcgca tccgtcgcat ccgcgggtgg ccgtagcggc tcaatcgcgg ccagcgccag
     5881 ccggggcaac ggcaccgcgt gcagccgctt tgtgcgccgt cgcaggcggt tgtcagccgg
     5941 aggcgtccag aaccccgagc cgtcgatctc acgccaccgc attgcagcaa gcgcgatctt
     6001 tctcttgccg gtcaggagga gaaccttgag gtaggcgccg gccgtgccgc tgatctcgtc
     6061 cgaggcctgc cagagcgctc ggagctcgtc gtcgttgaat accctgtctc gtgcctcttc
     6121 gcccttccac ggcttcctga ggccgatcat gggcgatgcc ttgaccttct ccacgccggg
     6181 ctcggcgcac cagccgaaga agcccgaaag caacgcatgg gtcttgaccg cgagatatgg
     6241 gcgaggcgcg gcgtcgtcgt cgccatcgcg gattgcctcg accagcgccc ggatgtctcg
     6301 gccgtcgatc tccgcgaccg gccgcgagag ccagccggca catgcccgca agaggatctt
     6361 ctggacctcg ccagcggtgg cgttctgcct ccgtcccttc tgctcgcgcg tgacgtagtc
     6421 ggcgaccgcc tcggcgaatg tctccgctcc cttgcggacc ttcgtctcgc tccgagccgc
     6481 atggatagcg gccatcgggt cttcgccgcg atcaaccgcc tgccgcagcc gcttcgcttc
     6541 cgctcgggcc gaggtcgtct tccaatccgg ccaccgcccg atcgtgaagc g
//
LOCUS       DAOTFI010000254         6542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13972_length_6542_cov_7.671651, whole genome shotgun sequence.
ACCESSION   DAOTFI010000254 DAOTFI010000000
VERSION     DAOTFI010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13972_length_6542_cov_7.671651"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..488)
                     /locus_tag="P5558_20170"
     CDS             complement(24..488)
                     /locus_tag="P5558_20170"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRY26696.1"
                     /translation="MEIAPWVSQLWTASIAPGEALLRLVVALALCGCLGFERELVGRA
                     AGLRTHMLVGLAAALLTIMAFEMFAWVRSLEERPTADPLRLLEAITAGVAFLAAGSIF
                     REKDRVRGLTTGGGLWMAGAIGIAAGQGLLGLAAMATVLAVIVIAVLRRIEP"
     gene            749..1543
                     /locus_tag="P5558_20175"
     CDS             749..1543
                     /locus_tag="P5558_20175"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRY26697.1"
                     /translation="MRSKLLTASLVALAAGQLWAQPSAAETAKEQTAKNEQAVDITVV
                     PLGNWRYDDLYAGAWSADRMLDEFAVYGEEGEDIGSIENLIIGDDGQVLAIIAQVGGF
                     WDIGDTHVSVPFDQVELAPGEARIPLTEDNADDYSIFGDAGFYYEETAQNVNLVDDDL
                     ETPAGILKATDLIGDYAYLSGNLRYGYVSDLLIRDGKVASVVVNAAGYGTPGYYAYPY
                     TIHGWGRGVNGWRYDLPYTEDDVAGMDTFDYDQMKYNMGGSASGEG"
     gene            complement(2150..3496)
                     /locus_tag="P5558_20180"
     CDS             complement(2150..3496)
                     /locus_tag="P5558_20180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRY26698.1"
                     /translation="MSRGRLFRREVAALARIALPLVIASIGQIAIMTTDLLMLGWLGP
                     EALAATALALSAFHPVMLLGIGIGTAVTPLAAAALARRQVREMRRVVRQGLWAAVPFS
                     LLALPALWWIGSAFVAIGQDAALSHEAERYMRGVAPGLVAILVWNVLRCYATALERTR
                     TVLVVTLAAIPLNALFNYGLIFGEFGLPRLEVMGAGIGSSLTNLVLACVMALIVATRH
                     PFRRHHVLGRLWRPDWATFRAIHRIGIPIGLFILLEVGVFAGSAQIMGYVGTLQLAAH
                     QIALQLASIAFMVPLGIGQAVTVRVALAHGRDEPGAVRNAGLAGMALATGFMTCTALA
                     FWLLPGPLIRPFLDGGPDAPRVLAFATAYLAIAAVFQIADGLQVTAAHALRGLQDTRV
                     PLVIAAIGYWGIAFPTAGLLGLATPLEGRGVWLALALGLAIAASMLIRRFLRLSLA"
     gene            3740..5266
                     /locus_tag="P5558_20185"
     CDS             3740..5266
                     /locus_tag="P5558_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HRY26699.1"
                     /translation="MTDAALTVRLNAQDNVVVARRAIGERTTLDAEKVTTTAKIPAGH
                     KIATSRIANGEPVRKYDQIIGFASADILPGDHVHVHNCLMGEFDRDYAIGSRAKPVKM
                     VPEAERDTFQGFVRKNGKVGTRNYIGVLSSVNCSASVCRFLQQNFPKEVLARYPNVDG
                     IVALTHSTGCGMADRGEGFAALQRTLWGYARHPNFAGILMIGLGCEVNQIDFLLEAYQ
                     IERGPFFQTMTMQDTGGTRKTVERATAIITGMLPHANEFGRQKVSASELTVALQCGGS
                     DAYSGITANPALGYATDLLVAQGGTGILAETPEIYGAEHLLTQRAVSEEVGRKLIERI
                     RWWEEYTSSNGGEMNNNPSPGNKAGGLTTILEKSLGAAAKGGTTNLAGVLRYAEMATT
                     RGFLFMDSPGYDPCSVTGQVASGSNIVAFTTGRGSCFGFKPAPSIKLATNSAMFERME
                     EDMDINCGDILSGGATIESKGREILDLLLRVASGEQSKSEALGIGDHEFIPWQIGAVM
                     "
     gene            5308..6240
                     /locus_tag="P5558_20190"
     CDS             5308..6240
                     /locus_tag="P5558_20190"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY26700.1"
                     /translation="MDSARVERKLTTILVADAVGYSRAMAADEAAALTALRAARAVFA
                     RLIERHRGRIFSKAGDGLLAEFPSVVEAVQCAIEVQRELAGRHAAPGATQFRIGVHLG
                     DVMVDGDDLFGDGVNLAARLEGMAEPGGVLISQPVYDQVHAKLEVGFDYLGPQRPKNL
                     GAEVPVYRVALDGTPRSGPHWGEHRHRAGAATPPPNGPAVGPAEGGGWPRIRRHAQLL
                     GVVWLGLVAIDVFTGAGAWAHWPGIAMLMLLGLEAAPLLTRSWFDATYARLAIITAGL
                     ALINLATWSGTLWVGWPALGLIVFEVLRRLRRRS"
     gene            6348..6491
                     /locus_tag="P5558_20195"
     CDS             6348..6491
                     /locus_tag="P5558_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26701.1"
                     /translation="MAKGQKRSNREIKKPKADKKTASVAPAGKVTTVMSQAKPGAAPA
                     KKG"
BASE COUNT         1120 a         2278 c         2302 g          842 t
ORIGIN      
        1 cctgccggac tgagcacgac cggctagggc tcgatccggc ggagcacggc gatgacgatg
       61 accgccagca cggtcgccat ggcggcgagg ccgagcagcc cctgcccggc ggcaatgccg
      121 atcgccccgg ccatccagag cccgccgccg gtggtcagcc cccgcacccg gtccttctcg
      181 cggaagatcg agccggcggc gaggaaggcc acgccggcgg tgatcgcctc gagcagacgc
      241 agcgggtcgg cggtcggccg ctcctcgagc gagcgcaccc aggcgaacat ctcgaaggcc
      301 atgatggtca aaagcgccgc ggccagaccc accagcatgt gggtccgcag acccgcagcc
      361 cggccgacca gctcgcgctc gaagccgagg cagccgcaga gcgccagcgc cacgacgagc
      421 cgcaggagcg cctccccggg ggcgatgctg gcggtccaga gctgggaaac ccagggggca
      481 atctccatcg gtcctcggcg cgatcgctct gtgcggcacc cgcggcgggc gaagtcgggc
      541 ctcgcttcgg tgccggggtc cgctcggtgc atcttcaacc gtccggaacg gtgacccgtt
      601 ccatccgccg acagccgcga acattaaatc cacccgaccg tggaaccggg cgcccgggtc
      661 gggattggca gggtggatgc ccgaacagag cctggcatcc ccgccgctcg cgtggcggcg
      721 gtcgtgttcg gaaggaaagg aggagaccat gcggagcaag ctcttgaccg cgagcctcgt
      781 cgcccttgcc gccggccagc tctgggcgca gccgagcgcc gcggaaaccg ccaaggagca
      841 aacggccaag aacgagcaag cggtcgacat caccgtcgtg cccctcggca actggcgcta
      901 cgacgatctc tacgccggcg cctggagcgc cgaccggatg ctcgacgagt tcgccgtcta
      961 cggcgaggaa ggcgaggaca tcggcagcat cgagaacctg atcatcggcg acgatggcca
     1021 ggtgctcgcc atcatcgccc aggtcggcgg cttctgggac atcggcgaca cccacgtctc
     1081 ggtgcctttc gatcaggtgg agctggcgcc gggcgaggcc cggattccgc tgaccgagga
     1141 caatgccgac gactactcca tcttcggcga cgccggcttc tactacgagg agaccgcgca
     1201 gaacgtgaac ctggtggacg acgacctcga gacgccagcc ggcatcctga aagcgacgga
     1261 cctgatcggc gattacgcct acctctccgg caatttgagg tacggctacg tcagcgacct
     1321 cctcattcgc gacggcaagg tcgcgtcggt cgtcgtcaac gccgccggct atggcacacc
     1381 cggctactat gcctatccct acacgatcca tggctggggc cggggggtga acggctggcg
     1441 ttacgacctg ccctacaccg aggacgacgt ggccggcatg gacacgttcg attacgatca
     1501 gatgaagtac aacatgggcg gctccgcctc cggcgaaggc tgagagcgcg aagccgttct
     1561 ctaccccgaa ggacgggcgg ggggcgcgcc atgcgcgccc cttttttcgc cctgccacga
     1621 cggttctgct cggggcgccg caccgcggcc gggcctgcca cccccgccgc cctgccgccg
     1681 tcccctctgg tccatcacgt ctgccatcac gaatgcttat ggcacccgcg gcagaggggc
     1741 cgcgccgggg caccggcgcg cccgccggcg tccccgaacg cccgggttgt cgcggattcc
     1801 gcgacagcga gtccgggaat gtcgcgcata ttgcgacata agcgatcgtg atgtcgcgga
     1861 ttccgcgaca gtcggcccgg gatgtcgcgt atttcgcgac aggcaccgac gaacgaaccc
     1921 gcggattcga cgatcgaacc cggcaaaacg acgatcgaac ccgaggattc gacgaacgaa
     1981 cccggccttc cgcgaacgaa cccggcatca gataactcgt tgcaacatcg agcttattcg
     2041 tcgcgaacgg gccgcggccc ggcgccgggc ggacccggga atcgaattat gcccaacgaa
     2101 ccggggcggc gcttgccccg gacagcgaaa taggttataa gtcctatgat caggcgaggc
     2161 tcagccgcag gaagcgccgg atcagcatcg acgccgcgat ggcgaggccg agcgccaggg
     2221 cgagccagac gccccgaccc tcgagcgggg tggcgagccc gagcaggccc gccgtgggga
     2281 aggcgatgcc ccagtagccg atggcggcga tgaccagcgg cacgcgggtg tcctgcaggc
     2341 cgcgcaaggc atgggcggcg gtcacctgca ggccgtcggc gatctggaag acggcggcga
     2401 tggcgagata ggccgtggcg aaggcgagca ccctgggcgc gtcggggccg ccatcgagga
     2461 aggggcggat gagcgggccc ggcagcagcc agaaggcgag cgccgtgcag gtcatgaagc
     2521 ccgtggccag cgccatgccg gcgaggcccg cgttgcggac ggcgccgggc tcgtcccggc
     2581 catgcgccag ggccacccgg accgtcaccg cctgaccgat gccgagcggc accatgaagg
     2641 cgatcgaggc gagctggagg gcgatctggt gggcggcgag ctgcagcgtg ccgacatagc
     2701 ccatgatctg ggcggagccg gcgaagacgc cgacctcgag gaggatgaag aggccgatgg
     2761 ggatgccgat ccggtggatg gcccgaaaag tcgcccagtc cgggcgccag agccgcccca
     2821 gcacatggtg gcggcggaag gggtgcctgg tcgcgacgat gagcgccatg acacaggcga
     2881 gcacgaggtt ggtcagcgac gagccgatgc ccgcacccat gacctcgagc cggggcaggc
     2941 cgaattcgcc gaagatcagg ccatagttga agagcgcgtt gagcgggatg gcggccagcg
     3001 tgacgacgag gaccgtgcgg gtgcgctcga gggcggtggc gtagcagcgg agcacgttcc
     3061 agacgaggat ggcgacgagg ccgggcgcca cgccgcgcat gtagcgctcc gcctcgtggc
     3121 tgagcgccgc gtcctggccg atggcgacga aggcgctgcc gatccaccag agcgccggca
     3181 gggccaggag gctgaacggc accgccgccc acaagccctg gcggacgacc cggcgcatct
     3241 cgcgcacctg ccggcgggcg agcgccgcgg cggcgagcgg cgtgaccgcc gtgccgatgc
     3301 cgatgccgag cagcatgacc gggtggaacg cgctcagcgc cagggccgtg gccgccagcg
     3361 cctcggggcc gagccagccg agcatcagga gatcggtggt catgatggcg atctggccga
     3421 tcgaggcgat caccagaggc agggcgatgc gcgccagcgc cgccacctcc cggcgaaaga
     3481 ggcggcccct ggacatggaa gcggcacgcg gcagcgtgac ccggggcgag gagcgcatcg
     3541 ctcgacctga tacggctggg aatggaagca tggacgcgga accgctcgtg attagccttt
     3601 cggacatccg ccgtcgagcg ctggcggccc ccgagcgctg gcggtccggg cggagttctg
     3661 cgaaactcgc aggcctgaca gacaaggcgg gcggcggccg gcttcgaggc cggcgcccgc
     3721 gctcgggagc agcctcatca tgaccgacgc agcactcacc gtccggctga acgcgcagga
     3781 caacgtcgtc gtcgcccgcc gggcgatcgg cgagcgcacc accctcgacg ccgagaaggt
     3841 gaccacgacc gcgaagatcc cggccggcca caagatcgcg accagccgga tcgccaatgg
     3901 cgagccggtg cgcaagtacg accagatcat cggcttcgcc agcgccgaca tcctgccggg
     3961 cgaccatgtc catgtgcaca actgcctgat gggcgagttc gaccgcgact acgccatcgg
     4021 cagccgggcc aagccggtca agatggtgcc cgaggccgag cgcgacacct tccagggctt
     4081 cgtccgcaag aacggcaagg tcggcacccg caactatatc ggcgtgctct cctcggtgaa
     4141 ctgctcggcc tcggtctgcc gcttcctcca gcagaatttc cccaaggagg tgctggcgcg
     4201 gtacccgaac gtcgacggga tcgtggcgct gacccactcc accggctgcg gcatggccga
     4261 ccggggcgag ggcttcgccg ccctgcagcg caccctctgg ggctatgcgc ggcacccgaa
     4321 cttcgccggc atcctgatga tcggcctggg ctgcgaggtg aaccagatcg acttcctgct
     4381 cgaggcctac cagatcgagc gcgggccgtt cttccagacc atgaccatgc aggacaccgg
     4441 cggcacgcgg aagaccgtcg agcgggcgac ggcgatcatc accgggatgc tgccgcacgc
     4501 caacgagttc ggccggcaga aggtctcggc ctcggagctc accgtggcgc tgcaatgcgg
     4561 cggatcggac gcctattcgg gcatcaccgc caacccggcg ctcggctacg ccaccgacct
     4621 cctcgtggcg cagggcggca ccggcatcct cgccgagacg cccgagatct acggtgccga
     4681 gcatcttttg acccagcgcg ccgtgagcga ggaggtgggc aggaagctga tcgagcggat
     4741 caggtggtgg gaggagtaca cgtcctccaa tggcggcgag atgaacaaca acccctcccc
     4801 cggcaacaag gcgggcgggc tcaccaccat cctcgagaag tcgctgggcg ctgccgccaa
     4861 gggcggcacc accaacctcg ccggcgtgct ccgctatgcg gagatggcga ccacgcgcgg
     4921 cttcctcttc atggacagcc ccggctacga cccctgctcg gtcaccggcc aggtggcctc
     4981 gggctccaac atcgtggcct tcacgacagg ccgcggctcc tgcttcggct tcaagccggc
     5041 gccgtcgatc aagctcgcca ccaactccgc catgttcgag cgcatggagg aggacatgga
     5101 catcaattgc ggcgacatcc tgagcggtgg tgccaccatc gagagcaagg gccgcgagat
     5161 tctcgacctg ctgctgcggg tggcctcggg cgagcagtcg aagtcggaag cgctcggcat
     5221 cggcgaccac gagttcattc catggcagat cggcgcggtc atgtgaccac gccggggggc
     5281 agatcggcgg atcggagctg atccgccgtg gactcggcgc gcgtcgagcg caagctgacg
     5341 accatcctcg tcgccgacgc ggtcggctac agccgggcca tggcggccga cgaggcggcg
     5401 gcgctcaccg cactccgggc agcgcgcgcc gtcttcgccc ggctgatcga gcggcaccgg
     5461 gggcgcatct tcagcaaggc cggcgacggg ctcctggccg agttcccgtc ggtggtcgag
     5521 gccgtgcaat gcgccatcga ggtgcagcgc gagctcgccg gccgccacgc tgcccccggc
     5581 gccacccagt tccgcatcgg cgttcatctc ggcgacgtca tggtggacgg cgacgatctc
     5641 ttcggcgacg gcgtcaacct ggcggcccgg ctcgagggca tggccgagcc cggcggggtg
     5701 ctgatctcgc agccggtcta cgaccaggtg cacgccaagc tcgaggtcgg cttcgactac
     5761 ctcggcccgc agcggccgaa gaacctgggc gccgaggtgc cggtctaccg ggtggcgttg
     5821 gacggcaccc cgcgaagcgg cccgcactgg ggcgaacatc gccatcgggc cggggcagcg
     5881 acgccgccgc ccaatgggcc cgcggtcggg ccggcggagg gcggcggctg gccccgcatt
     5941 cgccgccatg cgcagctcct gggtgtcgtc tggctcggcc tcgtggcgat cgacgtcttc
     6001 accggtgccg gcgcctgggc gcattggccg ggcatcgcca tgctgatgct gctcggcctc
     6061 gaggcggcac ccctgctcac gcgaagctgg ttcgacgcga cctatgcgcg cctcgcgatc
     6121 atcaccgccg ggctcgccct catcaacctc gccacctggt ccggcacgct ctgggtcggc
     6181 tggccggccc tcgggctcat cgtcttcgag gtcctccgcc gcctcaggcg gcggagctga
     6241 ctcgccgcca acaacagcgc cccccatggc gaatcactct cgttgacgga cgacccgcat
     6301 gctacaagcc cgggctgcgc gacgatgcgc aggcgaggag agttgccatg gccaagggcc
     6361 agaaacgcag caaccgtgag atcaagaagc cgaaggccga caagaagacg gcatcggtgg
     6421 cgccggccgg caaggtcacg acggtgatga gccaggccaa gcccggcgcg gcaccggcca
     6481 agaagggctg agcctgccgc cagagcggcg cgccctcgtc ctcagtgcga ggcgccgccg
     6541 tc
//
LOCUS       DAOTFI010000255         6536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_13991_length_6536_cov_7.699429, whole genome shotgun sequence.
ACCESSION   DAOTFI010000255 DAOTFI010000000
VERSION     DAOTFI010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13991_length_6536_cov_7.699429"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..787
                     /gene="rnc"
                     /locus_tag="P5558_20200"
     CDS             50..787
                     /gene="rnc"
                     /locus_tag="P5558_20200"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRY26702.1"
                     /translation="MKSRIANERPGDVAELEAAIAYRFADRLVLDEALTHASAMALGR
                     RRGGKAPRRSYERLEFLGDRVLGLAVAHLLIEAYPDEAEGGLTHRQSMLVCRETLADV
                     AEEIGLRHYLQVAHGGRGSGGLANPTILADACEALVGAVFIDGGYDAAAELVRRYWEG
                     RVAGLTSSPRDAKTMLQVWALARARELPSYTVLDSEGPAHAPVFTVEASLGGLGAATA
                     EAGSKRAAEQAAAALLLERLERGADDA"
     gene            777..1694
                     /gene="era"
                     /locus_tag="P5558_20205"
     CDS             777..1694
                     /gene="era"
                     /locus_tag="P5558_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRY26703.1"
                     /translation="MTPEPTAPETRAGFVAILGAPNVGKSTLLNRLVGTKVSIVSPKV
                     QTTRRRIIGISMVDEAQILFVDTPGIFTARKRLERAMVQAAWAAAADADLCLFLVDAR
                     RGLDADSRTVLEGLETSRRAPILVVNKIDLVSHERLLPLVKSLNEALAFDATFLVSAE
                     TGDGCPALLAAAAERLPAGPWLYPEDQLSDLSNRALAAEITREKLFLQLHQELPYSIT
                     VETEAWAEAPDGAEIRIDQVIYVQRPSQKGIVLGKGGRQIKSIGEAARLELEQILEAR
                     VHLFLFVKVRERWQEDAERYGEMGLDFPD"
     gene            1707..2444
                     /gene="recO"
                     /locus_tag="P5558_20210"
     CDS             1707..2444
                     /gene="recO"
                     /locus_tag="P5558_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRY26704.1"
                     /translation="MIEWRDEGILLSARPLGERDAVAAFLTFEHGRHAGLVRGGAGKR
                     QGALLQPGNRLALTWSARLDQHLGAFALEPMRLHAATIMGDGEALLALGAATALVDIG
                     SGERDPHPALYAALLKLIEALGPGRDWHETYVRFELVLLAELGFAVDLEACAVTGATD
                     DLAFVSPRTGRAVSNAAAGDLAARLLPLPGFLVGQGEADEESIAQGLRLAGHFLNRHI
                     LQPMDKAMPTARERLVLRLTHTTKDDG"
     gene            2444..4711
                     /gene="parC"
                     /locus_tag="P5558_20215"
     CDS             2444..4711
                     /gene="parC"
                     /locus_tag="P5558_20215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRY26705.1"
                     /translation="MAVAAPAGGSVVPIELKTALSERYLAYALSTITSRSLPDVRDGL
                     KPVQRRLLYAMLQLRLDPASAYKKCARVVGDVIGKYHPHGDVAVYDTLVRLAQAFSQR
                     YPLVDGQGNFGNIDGDNAAAMRYTESRLTAVAAALLYGIDEDTVDFRATYDGSEEEPV
                     VLPAAFPNLLANGSAGIAVGMATSIPPHNAAELFAALAFLLERPADAPPASVDELMAF
                     VRGPDLPTGGVLIEPASVIAEAYATGRGALRVRARWEVAKLAHGQYQIVVTEIPYQVQ
                     KARLIERLAELLAAKKLPLLADLRDESSEDIRLVLVPRSRGVPAELVMEQLFRQSELE
                     SRLSLNMNVLDAQGVPRVMGLLELLEAFIAHRLDVLVRRSRFRLGKIDDRLEILEAYL
                     EIFLDIDRVIRIIREEDEPKPVLMATFELNERQAEAVLNLRLRNLRKLEEMALEKERK
                     ALAKERRELAALLSDPAKRRATLAAAMRDGAETWGQGQLGPRRTELGAAPVLDPALME
                     LPVERYPVTVFCSAKGWVRTVRGHQGDTSEAKYKDGDAERFVIPAQSSDRLLAVATDG
                     RCYLVPVEKLPSGRGQGEPLSILIDLARGVDLLTLLVHRPDGRIALATRSGRGFVARE
                     SEIAAQTRGGRQVVNLEPDDRLAIAIPVTGDHVAVIGTNNKLLVFPLAELPEMSRGRG
                     VLLQRYKDGRLSDLATLDLADGLAWPMASGSRIRHEKDLGPWLGKRGQAGRNAPSGFP
                     RDQKFRPVPVEEGKG"
     gene            4786..>6536
                     /locus_tag="P5558_20220"
     CDS             4786..>6536
                     /locus_tag="P5558_20220"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY26706.1"
                     /translation="MALVLGAGLVLYSRQIGKAEAMAAAAELMDRSIEIVSLRADAFM
                     APIETAVGVAPEWPDVDRPPTLDGHPARKLLASLLQLLPQIASIELGYDSGDFYLISA
                     TRQRPEEQLERLGAPEAARFLEWYILRDGRATDLYVERFLDASLGVLSTRTRLGDAYD
                     PRSRPWFEAAQVTDGVAASDVYVFVGSGRQGLTLSRRHDTGVVGIDVTLEELDRHLRE
                     MPESEDGVLVIFDDDGEIVARSLPEGVAAGRREAVLAALVADALDGTPLAAEPIQIGE
                     EAWIAREASIDLGAERQQRVVVAMPVVEIVAPLAEAARGTALVSLLILAAGLPLLWLV
                     ARLLSRPLIRLAAEAEAVRRFDLELPVVAASRVGEIRQLERAMARMRASLRTFALYVP
                     KALVKQLVEQDDAPVIGGDRRDLTVLFMDLENFTGMAAELPPEEVMARMSRYFETVTR
                     ILLAHEATIDKYIGDAVMAFWNAPLDTADHPARACAAALAVVEAVRDETASWASAGTA
                     PVRTRIGIHSGPAIVGNVGSSDRMNYTALGATVNLAARLEQLNRNLGTEILASAAVVE
                     RVGDRFTFEHAGSTTLKG"
BASE COUNT          935 a         2411 c         2218 g          972 t
ORIGIN      
        1 gcgatccgct acaaccggct cttccggccc atcacctgat cgtttcgcca tgaaatcgcg
       61 catcgccaac gagcggccgg gcgatgtcgc cgagctcgag gcggccatcg cctatcgctt
      121 tgccgaccgc ctggtgctgg acgaggccct gacccatgcc agcgccatgg cgcttggtcg
      181 ccggcgtggc ggcaaggcgc cccggcgcag ctacgagcgg ctcgagttcc tgggcgaccg
      241 ggtgctcggg ctcgccgtgg cgcacctcct gatcgaggcc tacccggacg aggccgaggg
      301 cgggctgacc catcgccagt cgatgctggt ctgccgcgag accctggccg atgtggccga
      361 ggagataggc ctccgccact acctccaggt ggcgcatggc ggccgcggca gcggcgggct
      421 cgccaacccg accattctcg ccgacgcctg cgaggccctg gtgggcgccg tcttcatcga
      481 tggcggctat gatgccgcgg ccgagctcgt ccgccgctac tgggaaggcc gggtcgccgg
      541 cctgaccagc tcgccgcgtg acgccaagac catgctgcag gtctgggcgc tggcccgggc
      601 ccgcgagctg ccgagctaca ccgtgctcga cagcgagggg ccggcccatg cgccggtctt
      661 caccgtcgag gccagcctcg gcgggctggg tgccgcgacg gccgaggcgg gctcgaagcg
      721 ggccgccgag caggccgccg ccgccctgct gctggagcgg ctcgaaaggg gtgcggatga
      781 cgcctgaacc gacagcaccc gagacccgcg ccggcttcgt cgccatcctc ggcgccccca
      841 atgtcggcaa gtcgaccctg ctgaaccgcc tcgtcggcac caaggtctcg atcgtctcgc
      901 ccaaggtgca gacgacgcgg cggcgcatca tcggcatctc gatggtggac gaggcccaga
      961 tcctgttcgt cgacaccccg ggcatcttca ccgccaggaa gcgcctcgag cgcgccatgg
     1021 tgcaggccgc ctgggcggcc gccgcggacg ccgacctctg cctcttcctg gtcgacgcca
     1081 ggcgcggcct cgatgccgat agccgaaccg tgctcgaggg cctcgagacc agccgccgcg
     1141 cacccatcct ggtcgtcaac aagatcgacc tcgtgtcgca cgagaggctt ctgccgctcg
     1201 tcaagtcgct caacgaggcg ctcgccttcg acgccacctt cctcgtctcg gccgagaccg
     1261 gcgacggctg cccggcgctg ctcgccgccg ccgccgagcg cctgccggcc ggcccctggc
     1321 tctaccccga ggaccagctc tccgatctct ccaaccgggc gctcgccgcg gagatcacgc
     1381 gcgagaagct ctttcttcag ctccaccagg agctgcccta cagcatcacg gtcgagaccg
     1441 aggcctgggc cgaggcgccg gacggggcgg agatccgcat cgaccaggtg atctacgtcc
     1501 agcggccgag ccagaagggc atcgtgctcg gcaagggcgg ccgccagatc aagtcgatcg
     1561 gcgaggccgc gaggctcgag ctcgagcaga tcctggaagc ccgcgtccac ctcttccttt
     1621 tcgtcaaggt gcgcgagcgc tggcaggagg acgccgagcg ctacggcgag atggggctcg
     1681 attttccgga ctagcggcag gcggcgatga tcgaatggcg cgacgagggg atcctgctga
     1741 gcgcccggcc cctgggcgag cgcgatgcgg tggccgcctt cctcaccttc gagcacggcc
     1801 gccatgccgg cctcgtccgg ggcggtgcgg gcaagcggca gggtgccctg ctgcagccgg
     1861 gcaaccgact ggcgctgacc tggtcggcac ggctggacca gcatctgggc gccttcgcac
     1921 tcgagccgat gcggctgcat gcggccacca tcatgggcga tggcgaggcc ctcctcgctc
     1981 tgggtgccgc cacggccctc gtcgatatcg gctctggcga gcgcgacccg cacccggccc
     2041 tctatgccgc cctgctcaag ctgatcgagg ccctcggccc cggtcgcgac tggcacgaga
     2101 cctatgtccg cttcgagctc gtgctgctcg ccgagctcgg cttcgcggtc gacctcgaag
     2161 cgtgcgccgt caccggcgca acggacgatc tcgccttcgt ctcgccgcgc accggccggg
     2221 cggtcagcaa tgccgccgcc ggcgatctcg ccgcccgcct cctgccgctg ccgggcttcc
     2281 tcgtgggcca gggtgaagca gacgaggaat cgatcgccca ggggttgcgg cttgccgggc
     2341 atttcctgaa ccggcatatc ctccagccga tggacaaggc gatgccgacc gcgcgcgagc
     2401 gcctcgtcct caggctgacc cacacgacaa aggacgatgg ctgatggcag ttgcggctcc
     2461 agccggcgga tcggtcgttc cgatcgagct caagacggcg ctctccgagc gctacctcgc
     2521 ctatgcgctc tcgaccatca cctcgcgctc gctgcccgat gtccgcgacg ggctgaagcc
     2581 ggtccagcgg cgcctgctct acgccatgct gcagctccgc ctcgacccgg cctcggccta
     2641 caagaaatgc gcccgcgtcg tcggcgacgt gatcggcaag taccacccgc acggcgacgt
     2701 cgccgtctac gacaccctcg tccgcctggc ccaggccttc tcgcagcgct acccgctggt
     2761 cgacggccag ggcaatttcg gcaatatcga cggcgataac gccgccgcca tgcgctacac
     2821 cgagagccgt ctgaccgccg tggccgccgc cttgctctac ggcatcgacg aggacacggt
     2881 cgacttccgc gccacctatg acggcagcga ggaggagccc gtcgtcctgc cggcggcctt
     2941 ccccaacctg ctcgccaacg gctcggccgg catcgccgtc ggcatggcga cgagcatccc
     3001 gccgcacaac gcggccgagc tcttcgccgc gctggccttc ctcctggagc gaccggccga
     3061 cgccccgccc gcgtccgtgg acgagctcat ggccttcgtc cggggccccg acctgccgac
     3121 cggcggcgtg ctgatcgagc ccgcttcggt catcgccgag gcctacgcca ccggccgcgg
     3181 cgccttgcgg gtgcgcgccc gctgggaagt cgcgaagctc gcccacggcc aataccagat
     3241 cgtcgtcacc gagattccct accaggtgca gaaggcccgg ttgatcgaac ggctggccga
     3301 gctcctggca gcgaagaagc tgccgctgct cgcggacctc agggacgaat catcggaaga
     3361 catccgcctc gtcctggtgc cccgctcgcg cggcgtgccg gccgagctcg tcatggagca
     3421 gctcttccgc cagtccgagc tcgagtcgcg gctctcgctc aacatgaacg tgctcgacgc
     3481 ccagggcgtg ccgcgcgtca tggggctgct cgagctgctc gaggcgttca tcgcgcatcg
     3541 cctcgacgtg ctggtccgcc gctcgcgctt ccggctcggc aagatcgacg accggctcga
     3601 gatcctggaa gcctatctcg agatcttcct cgatatcgac agggtgatcc gcatcatccg
     3661 cgaggaggac gagcccaagc cggtgctcat ggccacgttc gagctgaacg agcgccaggc
     3721 cgaggccgtc ttgaacctgc gactgcgcaa tctgcgcaag ctcgaggaga tggcgctcga
     3781 gaaggagcgc aaggcgctcg cgaaggagcg ccgggagctc gccgccctgc tctccgatcc
     3841 ggcgaagcgc cgggcgaccc tcgccgccgc catgcgcgat ggtgccgaga cctgggggca
     3901 ggggcagctc ggccccaggc ggaccgagct gggtgccgcc ccggtgctcg atccggcgct
     3961 gatggagctg ccggtcgagc gctacccggt caccgtcttc tgctcggcca agggctgggt
     4021 caggaccgtg cgtggccacc agggcgacac cagcgaggcg aagtacaagg acggcgacgc
     4081 cgagcgcttc gtcattccgg cccagagcag cgacaggctg ctcgccgtgg cgacggacgg
     4141 ccgctgctat ctcgtcccgg tcgagaagct gccatcgggc cgcggccagg gcgagcccct
     4201 ctccatcctg atcgacctcg ccaggggcgt ggacctcctc accctcctgg tccaccgccc
     4261 ggacggccgc attgcgctcg ccacccgcag cgggcgtggc ttcgtcgccc gcgagagcga
     4321 gatcgcggcg cagacccggg gcggcaggca ggtggtcaat ctggagcccg acgaccggct
     4381 cgccatcgcc atcccggtca cgggcgacca tgtcgcggtg atcggcacca acaacaagct
     4441 cctggtcttt ccgctcgccg agctgcccga gatgagtcgc ggccgcggcg tgctcctgca
     4501 acgctacaag gacggcaggc tctcggatct cgcgaccctc gacctggcgg acggtctcgc
     4561 ctggccgatg gcctcgggca gccgcatccg ccacgagaag gatctcggcc cgtggctcgg
     4621 caagcgcggc caggccggtc gcaatgcgcc atccggcttc ccgagggacc agaagtttcg
     4681 cccggtgccg gtcgaggagg gcaagggctg accaaccgcc cggtctcgac cgagcgtcgc
     4741 gtccgcctga gcatcgccca ggccgtcctc gccgcgaccc tgctggtggc tctcgtgctc
     4801 ggcgccgggc tcgtcctcta ctcccggcag atcggcaagg ccgaggccat ggccgcggcg
     4861 gccgagctca tggaccgctc gatcgagatc gtcagcctgc gcgccgacgc cttcatggcc
     4921 ccgatcgaaa cggcggtcgg tgtcgcgccg gaatggcccg atgtcgatcg accgccgacc
     4981 ctcgacggcc atccggcccg caagctgctg gcttcgctgc tccagctcct gccgcagatc
     5041 gcctcgatcg agctcggcta cgacagcggc gacttctatc tgatcagcgc cacccggcag
     5101 cgtcccgagg agcagctcga acggctgggg gcacccgagg ccgctcgttt tctcgaatgg
     5161 tacatcctgc gcgacggtcg ggcgaccgac ctttatgtcg aacgcttcct cgatgcttcg
     5221 ctcggcgtcc tgagcacgag gaccaggctg ggcgacgcct acgatccgcg cagccggccc
     5281 tggttcgagg cggcgcaggt gaccgatggg gtggccgcga gcgatgtcta tgtgtttgtc
     5341 gggtctggcc gccagggcct gacgctgagc cgtcggcatg acacgggcgt ggtcggcatc
     5401 gacgtgacgc tggaggagct cgaccgacac ctccgcgaga tgcccgagtc cgaggacggg
     5461 gtgctcgtga tcttcgacga tgacggcgag atcgtcgccc gctcgctgcc ggagggggtc
     5521 gccgcgggca ggcgcgaagc cgtgctggcg gcgctcgtcg ccgacgcgct ggatggcacg
     5581 ccgctcgccg ccgagccgat ccagattggc gaggaggcct ggatcgcccg cgaggcgagc
     5641 atcgatctcg gcgccgagcg acagcagcgc gtggtcgtgg cgatgccggt ggtcgagatc
     5701 gtggccccgc tcgccgaggc cgcgcggggc acggcgctgg tctcgctcct gatcctggcc
     5761 gccggcctgc ccttgctctg gctggtcgcc cgtctcctct cccgaccgct gatccgcctc
     5821 gccgccgagg ccgaggccgt gcgtcgcttc gatctggagc tgccggttgt cgccgcctcc
     5881 cgggtcggcg agatccgcca gctcgagcgg gccatggcgc ggatgcgggc gagcctgcgc
     5941 accttcgcgc tctacgtgcc gaaggcgctg gtcaagcagc tcgtcgagca ggacgatgcg
     6001 cccgtcatcg gcggcgatcg ccgggatctc accgtgctct tcatggacct cgagaatttc
     6061 accggcatgg ccgccgagct gccgcccgag gaggtcatgg cgcgcatgtc gcgctacttc
     6121 gagacggtga cccggatcct gctggcccac gaagcgacca tcgacaagta tatcggtgac
     6181 gccgtgatgg ccttctggaa cgccccgctc gacacggccg accaccccgc cagggcctgt
     6241 gcggcggcgc ttgcggtggt cgaggccgtg cgcgacgaga cggcgagctg ggcgagcgcg
     6301 ggcacggccc ccgtccgcac ccgcatcggc atccattcgg gcccggccat cgtcggcaat
     6361 gtcggcagct ccgaccggat gaactacacc gcgctcggtg ccacggtgaa cctcgccgcc
     6421 cggctcgagc agctcaatcg caacctgggc acggagatcc tcgccagcgc cgccgtcgtc
     6481 gaacgggttg gcgaccggtt caccttcgag catgccggga gcaccacgct caaggg
//
LOCUS       DAOTFI010000256         6473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14183_length_6473_cov_7.321128, whole genome shotgun sequence.
ACCESSION   DAOTFI010000256 DAOTFI010000000
VERSION     DAOTFI010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14183_length_6473_cov_7.321128"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="P5558_20225"
     CDS             complement(<1..189)
                     /locus_tag="P5558_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26707.1"
                     /translation="MKPGGAPTTDEAAFQALGRLLPGGSASAARRLSRPLVFNRVAGA
                     ELMAEDGRSYIDLNCGYGA"
     gene            complement(269..1285)
                     /locus_tag="P5558_20230"
     CDS             complement(269..1285)
                     /locus_tag="P5558_20230"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY26708.1"
                     /translation="MTRARAPTLHTVARAAGVSTAIVSRVVNGHYHGMSAATRARVEA
                     AVDRLGYFPNRAARSLRTSRHHRIALIAVDSSPEFLADPFTTYVVAGLSNALSEQDYG
                     LLVQRLNRRAPLDMLLMRAQDADAITLFMSGPQDERLVLITRFRELGLPLLVFQETLA
                     PIPDDVCVVRQDDRAGGRWLGERIRGLGLSDVLAVVPELAWPAIEARLAGLRDGLDEP
                     GSLTILKVAETDMQAVSTALDRQLAAHVPEAIFASNDQLAAQSIGALARIGREVPADV
                     RVSGFNCLPITGTVHPPLTTIRSPAFALGTRAADLLVRRLAEGRFETKELVLPVEPQA
                     GQSL"
     gene            complement(1308..2591)
                     /locus_tag="P5558_20235"
     CDS             complement(1308..2591)
                     /locus_tag="P5558_20235"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY26709.1"
                     /translation="MTGVFLLVFLAFLLVVGTPIAFGVGIVAVAGIVLSGVGNPAVVA
                     QRAFAGINSYPLLALPLFVLAGNLLVSGGIIHRLVSFADLIVGRLRGGLAQVNIVSNL
                     MMAGISGSATADTAALGSVMVPMMQAKGYSTAFAAALTTSAAIVAPIIPPSLVMVIYA
                     IPTRLSIGAMFMAGILPGLLVTVLFMSYVAFATRHREPDAAAPPKEGIWRTLLAGLPV
                     LGAPVIIVGGVRGGVFTATEAAAVLVGYVLLLTLVVYRSLTLARLATTLRESALTTAV
                     VMFVVATSTIFAWYLAIENVPGLVRDLFVSLTDNTHVFLLLVNIFLLVVGCFVDTVPA
                     ILIFVPILQPAAAGFGIDPIHFSIIVILNLMVGLNTPPIGTNLFVIASVAKLRIGQVS
                     KALIPFFMIKLVALGIVTYWPELSLALPRSMGLIR"
     gene            complement(2588..3139)
                     /locus_tag="P5558_20240"
     CDS             complement(2588..3139)
                     /locus_tag="P5558_20240"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY26710.1"
                     /translation="MPGSNEPETPGATPRASPARPAGPAVRAFRWLVDAILVTALAVI
                     SIVLICQVVLRYVFHAALPWPEELAQFLLVLLTLLGGYRALEMDMHIRLQVLDDFRWP
                     RLVMLLRLAGYAASAAFMLYVAYGGWDLTMRSMHTPSTALRMPMGVVYATLPIAFGLM
                     TILLVGIMVRLVRGRDVVLDRPE"
     gene            complement(3123..4148)
                     /locus_tag="P5558_20245"
     CDS             complement(3123..4148)
                     /locus_tag="P5558_20245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY26711.1"
                     /translation="MIKRLALTAAIGALGFVPAADAADFTIRASHAESTESPLHKGWL
                     VFAAYVEAASGGQIEVEVLPAGQLGSIHDTLEQAQLGAIEVAQGDEANLDPFFAPMLF
                     LSTPYLFSDGDEALEFLRRPFFQELSERMAAESGLRLLAAAPYGFRSFTNNVRPLKTA
                     ADLEGIRMRVPPSPMSLAMVEAMGGSPTPVPWEELYGALQQGVVDGQENPVGIVFDYT
                     FYQVQKYLTVDNHQLGLNTIMINEDFFQSLPPELRQVVVAGAQEGAMTEFGERNYQAR
                     VSAVEALRAEGMEVYLPSAEERATFRDATIEPIRAYLTGELGAEFVDALYAEVEALRA
                     ERLARVE"
     gene            complement(4213..4872)
                     /locus_tag="P5558_20250"
     CDS             complement(4213..4872)
                     /locus_tag="P5558_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRY26712.1"
                     /translation="MAIDFAKLRKELYTAVLSDTLDALGHTHQAMKPFVRPLDDRSVL
                     FGRARTGLFMPIYHIPEGENFYELEIRLIDDLKPDEVAVFACNGPTDRIAPWGELLST
                     AASYRGAAGCVTDGLVRDVRQIRAMEFPVFHGGIGPLDSQGRGRVVAIDVPVECGGVA
                     VRSGDLVFGDVDGVVVIPQAIEGAVLRAALEKIKGENRTRDELAKGLKLADVYAKYGV
                     L"
     gene            4989..6383
                     /locus_tag="P5558_20255"
     CDS             4989..6383
                     /locus_tag="P5558_20255"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY26713.1"
                     /translation="MTLRVAQTNHQADRLLLGFSDLRRLRSERPLVFERGRGIFIIDE
                     RGKDYIEAVSSFYCATLGFSDEELAEAASRQLRTLPMYPSAIHRTTTPVLELAEKLAA
                     IAPIPNARIHFSTTGSEVNDHLIKFMWYGNHFADEPLRRKIISRLGSYHGSTIATAAL
                     GGGSDLHASFALPMQESVLVSHPAWPNAAHPGEDEAAFTDRLAEELTQAIEAAGPETV
                     GGMIAEPVSVSCGMFPPPESYFPKVKAVLDRYGIRLFVDEVVTGFGRSGHMWASEAKG
                     LEPDTVSCAKGISGAYMPLAALIMGEAFADRLERGSDTKGWFAHGGTHQGHTVSAAVG
                     LKVLEIFERRDILGHVQRLIPYWNRTLDRMTAHPLVAGSRRFGLMGALDVALPGAGEK
                     DLAASLAVGGLSKAVYEAGLEHGIIVRPLAGCIVMAPPLIITEAEIDELARRLEAALD
                     QVLADTKKVPETVG"
BASE COUNT         1114 a         2160 c         2290 g          909 t
ORIGIN      
        1 ggcgccatag ccgcaattga ggtcgatata gctgcggccg tcctcggcca tcagctcggc
       61 accggcgacc cggttgaaca cgaggggacg tgagagacgg cgcgcggcgc tggcgctgcc
      121 gcccggcaac aggcgaccga gtgcctgaaa cgcggcctcg tccgttgtcg gcgcgccacc
      181 cggcttcatg cttttcccca tacccgttcc ttcctcgata gccttcgacc ccgtggaccg
      241 aaagcgctat ttgccgtcgt gccccggctc acagcgactg tccggcctgc ggctccaccg
      301 gcaggaccag ctccttcgtc tcgaaccggc cctcggcgag gcgccgcacc agaaggtcgg
      361 cggcgcgcgt gccgagcgcg aaggccggcg agcggatggt ggtcaggggc gggtggaccg
      421 tgccggtgat cggcaggcag ttgaacccgc tgacccgcac gtccgccggc acctcgcggc
      481 cgatccgtgc gagggccccg atcgactggg cggcgagctg gtcgttgctg gcgaagatcg
      541 cctcgggcac gtgtgctgcg agctgccggt cgagcgctgt cgagacggcc tgcatgtcgg
      601 tctccgccac cttcaggatg gtcaggctgc ccggctcgtc cagcccgtcg cgcaggcccg
      661 cgagcctcgc ctcgatggcc ggccaggcga gctcgggcac gaccgccagg acgtccgaga
      721 gacccaggcc gcgaatgcgc tcgccgagcc agcggccgcc cgcccggtcg tcctggcgga
      781 cgacgcagac atcgtccggg atcggcgcca gcgtctcctg gaagaccagg agcggcaggc
      841 cgagctcgcg gaaccgggtg atcagcacca gccgctcgtc ctgcgggccg gacatgaaga
      901 gcgtgatggc atcggcatcc tgggcgcgca tcaggagcat gtcgaggggt gcccggcggt
      961 tcagccgctg gacgaggagg ccgtaatcct gctccgagag cgcgttggag aggccggcca
     1021 cgacataggt ggtgaacggg tcggcgagga actccggcga gctgtcgacg gcgatcagcg
     1081 cgatccggtg gtgccgactg gtgcgcagcg agcgcgcggc ccggttgggg aagtagccga
     1141 gcctgtcgac cgccgcctcg acccgtgccc gggtggccgc cgacatgccg tgatagtggc
     1201 cgttgacgac gcgcgagacg atggccgtgg agacaccggc ggcacgcgcc accgtgtgca
     1261 gggtgggtgc ccgggcgcgg gtcatggtgc cggggagggg gccgccgtca acggatgagc
     1321 cccatggagc gcggcaaggc cagggagagc tccggccaat aggtgacgat gcccagcgca
     1381 accagcttga tcatgaagaa cgggatgagc gccttcgaga cctggccgat ccgcagcttc
     1441 gccaccgagg cgatgacgaa gaggttggtg ccgatgggcg gcgtgttcag cccgaccatg
     1501 aggttgagga tgacgatgat cgagaagtgg atcggatcga tgccgaagcc cgccgctgcc
     1561 ggttgcagga tcggcacgaa gatcaggatg gccggcaccg tgtcgacgaa gcagccgacg
     1621 accaaaagga agatgttcac cagcagcagg aagacatgcg tgttgtcggt gagcgagacg
     1681 aagagatcgc gcaccaggcc cggcacgttc tcgatcgcca gataccaggc gaagatggtc
     1741 gaggtggcga cgacgaacat cacgaccgcg gtggtgaggg cactctcgcg cagcgtggtg
     1801 gccagccgtg ccaaagtgag cgagcggtag acgacgagcg tcagcaggag gacgtagccg
     1861 accagcacgg cggcggcctc cgtcgccgtg aagacaccgc cgcgcacgcc accgacgatg
     1921 atcaccgggg cacccagcac aggtagaccg gcgagcagcg ttcgccagat gccctccttc
     1981 ggcggggcgg cggcatcggg ctcgcggtgg cgggtggcga aggcgacata gctcatgaag
     2041 agcacggtga cgagcaggcc cggcaggatg ccggccatga acatcgcccc gatcgagagc
     2101 cgggtcggga tcgcgtagat caccatgacg aggctcggcg ggatgatcgg ggcgacgatc
     2161 gcggccgagg tggtgagcgc ggcagcaaag gcggtggaat agcccttcgc ctgcatcatc
     2221 ggcaccatca ccgagccgag cgctgccgtg tcggcggtgg ccgagccgga gatgccggcc
     2281 atcatcaggt tggagacgat gttgacctgg gcgaggccgc cccggagccg gccgacgatg
     2341 agatcggcga agctgacgag gcgatggatg atgccgccgg agacgaggag gttgccggcg
     2401 aggacgaaga gcggcagggc gagcagggga tagctgttga tgccggcgaa ggcgcgctgc
     2461 gccacgacgg ccgggttgcc gacgccggag agcacgatgc cggcgacggc gacgatgccg
     2521 accccgaacg cgatgggcgt gccgacgacg agcaggaagg ccaggaagac cagcaggaag
     2581 acgccggtca ttccgggcga tccagcacca catcccgccc gcgcaccagg cgaaccatga
     2641 tcccgaccag caggatggtc atcagcccga aggcgatcgg cagggtcgca tagacgacgc
     2701 ccatgggcat gcggagcgct gtcgacggcg tgtgcatgct gcgcatcgtc agatcccagc
     2761 cgccataggc gacgtagagc atgaaggcgg cgctggcggc gtagccggcg agccgaagca
     2821 gcatgaccag ccgcggccag cggaaatcgt ccagcacctg caggcggatg tgcatatcca
     2881 tctcgagggc gcggtagccc ccgagcaggg tcaggaggac gaggaggaac tgggccagct
     2941 cctccggcca gggcagcgcc gcatgaaaga cgtaacgcag caccacctgg cagatcagca
     3001 cgatcgagat gaccgcaagc gccgtcacca ggatggcgtc gaccagccag cgaaaggccc
     3061 gaaccgccgg gccggcaggt ctggcgggcg acgcccgggg ggtggccccg ggcgtctctg
     3121 gctcattcga cccgggcaag tcgctcggcc cgaagcgcct cgacctccgc gtagagtgcg
     3181 tcgacgaact cggcgccgag ctcgcccgtg agataggcgc ggatcggctc gatcgtcgcg
     3241 tcacggaagg tggcgcgctc ctcggccgac ggcaggtaga cctccatgcc ctcggcgcgc
     3301 agtgcctcga cggcgctgac ccgggcctgg tagttgcgct cgccgaactc ggtcatcgcc
     3361 ccttcctggg caccggcgac cacgacctgg cgaagctcgg gcggcaggct ctggaagaag
     3421 tcctcgttga tcatgatggt gttgaggccg agctggtgat tgtcgaccgt gaggtacttc
     3481 tgcacctggt agaaggtgta gtcgaagacg atgccgacgg ggttctcctg gccgtccacg
     3541 acaccctgct gcaaggcacc atagagctcc tcccagggca ccggcgtcgg cgagccgccc
     3601 atcgcctcga ccatggcgag cgacatcggg ctcggcggca cgcgcatgcg gatgccctcg
     3661 agatcggccg ccgtcttcag cggccgcacg ttgttggtga acgagcggaa gccgtagggt
     3721 gcggcggcga gcaggcgcag gcccgattcg gccgccatcc gctcgctcag ctcctggaag
     3781 aacggccggc gcaggaactc gagcgcctcg tcgccatccg agaagagata gggcgtggag
     3841 aggaacagca tgggcgcgaa gaaggggtcg agattggcct cgtcgccctg cgccacctcg
     3901 atggcgccga gctgcgcctg ctcgagcgtg tcatggatcg agccgagctg gccggccggc
     3961 agcacctcga cctcgatctg gccgccgctc gccgcctcga cataggcggc gaagaccagc
     4021 cagcccttgt gcagcgggct ctcggtcgat tccgcgtggc tggcgcggat ggtgaagtcc
     4081 gccgcgtccg cggccgggac gaagcccagc gcaccgatgg ccgcggtgag cgcgagacgt
     4141 ttgatcacga cgtttccctc ctggttggac gacttggaat tgacgacggc gatcggatcg
     4201 cggtcggcgc gttcagagca cgccgtattt cgcgtagacg tcggcgagct tgagcccctt
     4261 ggccagctcg tcgcgcgtcc ggttctcgcc cttgatcttc tcgagcgccg ccctcagcac
     4321 cgcgccctcg atcgcctgcg ggatgaccac cacgccatcc acatcgccga agacgaggtc
     4381 gcccgagcgg acggcgacgc cgccgcactc gaccggcacg tcgatcgcga ccacgcgccc
     4441 gcggccctgg ctgtcgagcg ggccgatgcc gccgtggaag accgggaact ccatggcccg
     4501 gatctgccgg acgtcacgca ccagcccgtc ggtgacgcag cccgcggcac cccggtagct
     4561 ggcggcggtc gacaggagct cgccccaggg ggcgatgcgg tcggtcgggc cgttgcaggc
     4621 gaagaccgcg acctcgtcgg gcttcagatc gtcgatcagc ctgatctcga gctcgtagaa
     4681 gttctcgccc tcggggatgt gatagatcgg catgaagagg ccggtgcggg cgcgcccgaa
     4741 cagcaccgac cggtcgtcca gcgggcgcac gaacggcttc atcgcctgat gggtgtgacc
     4801 gagcgcgtcc agcgtgtcgg agaggacggc ggtgtagagc tccttgcgga gtttcgcgaa
     4861 gtcgattgcc acgtcattca tccaagatta aacgtttaac ctgtcctagt gcgttgcgga
     4921 tcgctgtcaa tcgcgcttgt cgtggcgcac cgtgccgccg cacgctggcc ctgacccggg
     4981 agagacccat gacgcttcgc gtggcgcaga ccaatcacca ggccgaccgc ctgctgctgg
     5041 gcttcagcga cctgcgccgc ctgcgcagcg agcggccgct ggtgttcgag cgcggccggg
     5101 gcatcttcat catcgacgag cgcggcaagg actatatcga ggcggtctcg tccttctatt
     5161 gcgccaccct cggcttcagc gacgaggagc tggccgaggc ggccagccgc cagctgcgca
     5221 ccctgccgat gtatccctcg gcgatccacc ggaccacgac gccggtgctc gagctcgccg
     5281 agaagctggc agccatcgca cccattccca acgcccgcat ccatttcagc accacgggct
     5341 cggaggtgaa cgaccacctc atcaagttca tgtggtacgg caaccacttc gccgacgagc
     5401 ccctgcgccg caagatcatc tccaggctcg gcagctacca cggcagcacc atcgccacgg
     5461 cggcgctggg cggtggcagc gacctgcatg ccagcttcgc cctgccgatg caggaatcgg
     5521 tcctggtctc gcatccggcc tggcccaacg cggcccaccc gggcgaggac gaggcggcct
     5581 tcaccgaccg gctggcggaa gagctgacgc aggcgatcga ggccgccgga ccggagacgg
     5641 tgggcgggat gatcgccgag ccggtctccg tctcctgcgg catgttcccg ccgcccgaaa
     5701 gctacttccc caaggtgaag gcggtcctcg accgctacgg catcaggctc ttcgtcgacg
     5761 aggtggtcac cggcttcggc cgtagcggcc acatgtgggc gagcgaggcc aaggggctcg
     5821 agcccgacac cgtctcctgc gccaagggca tctccggcgc ctacatgccg ctcgccgccc
     5881 tcatcatggg cgaggcgttc gccgaccggc tggagcgcgg cagcgacacc aagggctggt
     5941 tcgcgcatgg cggcacgcat cagggccaca cggtctccgc cgccgtcggc ctcaaggtcc
     6001 tcgagatctt cgagcgccgc gacatcctcg gccatgtcca gcgcctcatc ccctactgga
     6061 accgcacgct ggaccggatg accgcgcacc cgctggtcgc cggcagccgc cggttcggcc
     6121 tgatgggtgc cctcgacgtg gccctgccgg gtgccggcga gaaggacctg gccgccagcc
     6181 tcgccgtcgg cgggctcagc aaggcggtct atgaggccgg gctggagcac ggcatcatcg
     6241 ttcgcccgct cgccggctgc atcgtcatgg caccaccgct gatcatcacc gaggccgaga
     6301 tcgacgagct ggcccgccgg ctcgaggccg cgctcgacca ggtcctggcc gacacgaaga
     6361 aggttcccga aaccgttggt tgatgcggca agtgacggta gaaaagctag caataactgt
     6421 tagttagcca gtgtatgttt gcaggccaac caaggcccat aagcgaatcg taa
//
LOCUS       DAOTFI010000257         6363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14492_length_6363_cov_6.632530, whole genome shotgun sequence.
ACCESSION   DAOTFI010000257 DAOTFI010000000
VERSION     DAOTFI010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14492_length_6363_cov_6.632530"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="P5558_20260"
     CDS             <1..1167
                     /locus_tag="P5558_20260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY26714.1"
                     /translation="SPPRTEQLRAMVDQFEAANPGVTVEITSLPWGQAFERLATMVQG
                     GQTPDVVEMPDRWLSLYANAGQLVDLTPFLAEWDGTAELTDATKSFGSVISGQTHMIP
                     YGFYLRAMFWNKKLFAEAGLDRAPETLQEFYDFSAKIAELDGKYGYCLRGGPGATNGY
                     IMMMLNFLGENKYFNEDGTSTLDDPKAIEGLQFIVDMYQNGLVPRDSVNWGFNEIVAG
                     FYSGTCAMLDQDPDALIAIKERMPAEDFAVAPMPLGPAGKSFPTIGYAGWSIFEASEH
                     KDVAFDLIAHLSSREMNLEWSKFVGVIPIHVGAEQDPFFTGPNFEGWFVEINDDRWEP
                     TSMPTYLEEFGYFADVVSIQGGQEALLGQRTAEEVATEWADYLTPAQQKWLAAQ"
     gene            1176..2084
                     /locus_tag="P5558_20265"
     CDS             1176..2084
                     /locus_tag="P5558_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY26715.1"
                     /translation="MTGRAGRGTGRLALALEPWVYLSPALVMLGLVMFVPLVVGFTYA
                     FQDIRLLNPLAGGWVGLKNFETLAGDKNFRVALVNTVWWTVGSVALQFLFGLGLAMLL
                     NRAFWGKRLVQAVVFLPWAVPTFLSGLTFAWLLNPVIGPVPHWLFAMGILEEPYNILG
                     NPDLAMWGPIAANVWFGVPFFAITLLAALQAIPSEMYESAAIDGATAWQGFVQVTLPY
                     LAPMIAITVLLRTIWIANFADLIIVMTNGGPANATQILPSYIFTTAYRKLDFGYASAI
                     AMALLLLLFLYAVLLHQMRKVFRSTA"
     gene            2081..2932
                     /locus_tag="P5558_20270"
     CDS             2081..2932
                     /locus_tag="P5558_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY26716.1"
                     /translation="MTSFTRPLHHQLALALGHRLGILLYLLVALFPVYWLIKIAVTPE
                     ALLYSEGVRLWPSSFTFDNFSKVLLASNFPAFFRNSVIVSFGTAIAVTAIASAAGYAF
                     SRFAFKGRAAIVFVMLLTQMFPLVLLIAPIYRLMTPLGLTDSLIGLIIVYTAFNTPFA
                     TFLMQSFLEGIPKELEEAAMIDGCTRPQALIRVILPLTLPGMGATLGFVFTAAWSELL
                     FCLMLINSEAKMTFPVGLLSFVSKFSVDWGQMMAAAVLALIPACIFFAFIQRYLVQGL
                     TAGAVKG"
     gene            3000..4193
                     /locus_tag="P5558_20275"
     CDS             3000..4193
                     /locus_tag="P5558_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRY26717.1"
                     /translation="MIENRLARRVFMQRHGLLDPPGRKLGKAGLLEAIDRLGFVQVDS
                     INTVERAHHMILLGRSQTYQPRHLKALVEKDRALFEHWTHDASIIPTSFYPYWQRHFA
                     RTTERLRGRWQRWHGYDFDAQAEGVLEHIARHGPVLARELNEPDPDRPRRQGWWAWHP
                     SKKALEYLWRTGRLAVCRRDGFQKVYDLVERVIPEAHRHDGIDEAALVDWACRAALDR
                     LGFATSGEIAAFWGIVTPAEARTWCAAAAHGAVITSIESADGSAPRKVLADAALLDLH
                     SDDLAPPPRIRVLSPFDPLVRDRDRALRLFDFDYRIEVFVPEAKRRWGYYVFPLLEGD
                     RFVGRIDMRAERGTGTLAVRRLWWEPGIRATRGRLGRLEAELARVARFAGCDRVRFDE
                     HPGAS"
     gene            complement(4203..>6363)
                     /locus_tag="P5558_20280"
     CDS             complement(4203..>6363)
                     /locus_tag="P5558_20280"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26718.1"
                     /translation="DGRRLEGRGREGAASTGGAGAAGVAPTPESIAAAQEAAIDEGLA
                     AYNRGDHATAYRLFRPIAELGNARAQNNLGALYDQGHGVARNDAEAVLWFRRSAAQGH
                     RLGQHNLARHLLDGRGVAVDIDEAARLFRLSAEQGHAPAANELGRLHYDRRIAGADDG
                     EAVKWFRRAALAGDPWAQFNLGLAYENGRGVLPNPEAAWQWFGKSAESHAPGDEAAAA
                     TARDRVLALLSRDVTAEITFNASRTQTADTYDVQAVTAYEMLAAGEYRLALFGFNLAL
                     AEMEDHGQTTAWNSTLEDVYWARIEALTGLGRIEEADATWLALARNDWWTIFLDPETA
                     LEVTNRLLAREPDNPGYLALRAEAHAELGDDVARFADADRRVALETNDYLRADALLQR
                     SGAHVALGRPAEALADAEAAVGLDPSSDNLAHRAERHRALGHAAEARDDYTAAIEGLA
                     EDDWRLEDYRLARANLAVELGDPAAALVDLDAVIAADGGVDVTERARLALRTEVLVTL
                     GLDERAAVDLEELTKRDAAFVETALRPKLDAIRAERPSPSTDARDPAAGGQAWKLGGM
                     LAVGTLRYMLSGEAEPLDIWRAASGFAQTLGLTIPPLPERGDTARANLEAALDYLKTA
                     NGDTANAILVQHDPRTAYVFRLAVDIGVLATVHGFALDELNARLVDSAIVDGLFSGVP
                     EEVYQPFLDAVTNGADRATLDAALRELDEKSARFFEG"
BASE COUNT          877 a         2279 c         2134 g         1073 t
ORIGIN      
        1 agcccgccca ggaccgagca attgcgggcc atggtcgacc agttcgaggc ggccaatccg
       61 ggcgtgacgg tggagatcac gtccctgccc tggggccagg ccttcgagcg gctggccacc
      121 atggtgcagg gcgggcagac accggacgtg gtggagatgc cggatcgctg gctctcgctc
      181 tatgccaatg cgggccagct cgtcgatctg acgccttttc tcgccgagtg ggacggcacg
      241 gccgagctga ccgatgcgac caagtcgttc ggcagcgtca tctcgggcca gacccacatg
      301 attccttacg gcttctatct gcgggccatg ttctggaaca agaagctctt cgccgaggcc
      361 gggctcgacc gcgcacccga gacgctgcag gagttctacg acttttccgc caagatcgcc
      421 gagctcgacg gcaagtacgg ctattgcctg cgcggcgggc cgggcgcgac caacggctac
      481 atcatgatga tgctgaactt cctgggcgag aacaagtact tcaacgagga cggcacctcg
      541 acgctcgacg accccaaggc gatcgagggc ctgcagttca tcgtcgacat gtaccagaac
      601 ggcctggtgc cccgggacag cgtcaactgg ggcttcaacg agatcgtcgc cggcttctat
      661 tcgggcacct gcgccatgct cgaccaggac ccggatgcgc tgatcgccat caaggagcgg
      721 atgccggcgg aggacttcgc cgtcgccccc atgccgctcg gcccggccgg caagtccttt
      781 cccaccatcg gctatgccgg ctggtcgatc ttcgaggcct cggagcacaa ggacgtcgcc
      841 ttcgacctga tcgcgcatct gtcgtcgcgc gagatgaacc tcgaatggtc gaaattcgtc
      901 ggcgtcatcc cgatccatgt gggggcggag caggatccgt tcttcaccgg gccgaacttc
      961 gagggctggt tcgtcgagat caacgacgat cgctgggagc ccacctcgat gccgacctat
     1021 ctcgaggagt tcggctactt cgccgacgtc gtctcgatcc agggcggcca ggaggcgctg
     1081 ctcggccagc gcaccgccga ggaggtcgcc accgagtggg ccgactacct gaccccggcc
     1141 cagcagaagt ggctggcagc acagtagcgg ggcccatgac ggggcgggcc ggccggggca
     1201 ccggtcggct cgccctggca ctcgagccct gggtctatct ctcgccggcg ctcgtgatgc
     1261 tcgggctggt catgttcgtg ccgctcgtcg tcggcttcac ctacgctttc caggacattc
     1321 gcctgctcaa cccgctcgcc ggcggctggg tggggctgaa gaacttcgag accctggccg
     1381 gcgacaagaa tttccgcgtc gcgctcgtca acaccgtctg gtggacggtg ggctcggtgg
     1441 cgctgcagtt ccttttcggc ctcggcttgg cgatgctgct caaccgcgcc ttctggggca
     1501 agcggctcgt ccaggccgtg gtcttcctgc cctgggccgt gccgaccttc ctctcgggcc
     1561 tgaccttcgc ctggctgctc aacccggtga tcggtccggt cccgcactgg ctcttcgcca
     1621 tgggcatcct cgaggagccc tacaacatcc tgggcaaccc cgacctcgcc atgtgggggc
     1681 ccattgccgc caatgtctgg ttcggcgtgc ccttcttcgc catcaccctg ctcgccgccc
     1741 tgcaggccat cccgagcgag atgtacgaat ccgccgccat cgacggcgcc acggcctggc
     1801 agggcttcgt ccaggtgacg ctgccctatc tggcgccgat gatcgccatc accgtgctgc
     1861 tgcggaccat ctggatcgcc aacttcgccg atctcatcat cgtcatgacg aatggcggcc
     1921 cggccaacgc cacgcagatc ctgccctcgt acatcttcac gaccgcctat cggaagctcg
     1981 acttcggcta tgcttcggcc atcgccatgg cgctcttgct cctgctcttc ctctatgccg
     2041 tgctgctcca ccagatgcgc aaggtcttcc ggagcacggc atgacgagct tcacccggcc
     2101 gctgcaccac cagctcgcct tggccctcgg ccaccggctc ggcatcctgc tctatcttct
     2161 cgtcgccctc ttcccggtct actggctcat caagatcgcg gtgacgcccg aagccctgct
     2221 ctattccgaa ggggtccgcc tctggccctc gtccttcacc ttcgacaact tttccaaggt
     2281 gctgctggcg agcaattttc cggccttctt caggaactcg gtgatcgtct ccttcggcac
     2341 cgccatcgcg gtcacggcca tcgccagcgc tgccggctac gccttctcgc gcttcgcctt
     2401 caagggccgg gcggccatcg tcttcgtcat gctgctgacg cagatgttcc cgctcgtgct
     2461 gctgatcgcg ccgatctacc ggctgatgac gccccttggc ctaaccgact ccctgatcgg
     2521 cctgatcatt gtctacacgg ccttcaacac gcccttcgcc accttcctca tgcagtcctt
     2581 cctcgagggc atccccaagg agctcgagga ggcggcgatg atcgacggct gcacgcggcc
     2641 gcaggcgctg atccgggtca tcctgccgct caccctgccg ggcatggggg cgacgctcgg
     2701 cttcgtcttc accgccgcct ggtcggagct gctcttctgc ctcatgctga tcaacagcga
     2761 ggcgaagatg acctttcccg tgggccttct ctccttcgtc tcgaaattct cggtcgactg
     2821 ggggcagatg atggcggccg ccgtgctggc gctgatcccg gcctgcatct tcttcgcgtt
     2881 catccagcgc tatctggtcc agggcctgac cgccggcgcg gtcaagggct gagggccgaa
     2941 cgaccggaat cgatggcggc aggcacgcag cgacgacagg gcgaagggcg agcctcggca
     3001 tgatcgagaa ccgcctcgcc cgccgcgtct tcatgcagcg ccacggcctg ctcgacccgc
     3061 cgggccgcaa gctgggcaag gccgggctgc tcgaggcgat cgaccggctg ggcttcgtcc
     3121 aggtggacag catcaacacg gtcgagcgcg cccaccacat gatcctcctg ggccgcagcc
     3181 agacctacca gccccggcac ctgaaggcgc tggtcgagaa ggaccgtgcg ctcttcgagc
     3241 actggacgca tgacgcctcg atcatcccca catccttcta tccctactgg cagcgacact
     3301 tcgcccgcac gaccgagcgc ctgcgcggcc gctggcagcg ctggcacggt tacgatttcg
     3361 acgcgcaggc cgagggcgtg ctggagcaca tcgcgaggca cggccccgtg ctcgcccgcg
     3421 agctgaacga gcccgacccc gaccggccgc gccgccaggg atggtgggcg tggcatccat
     3481 ccaagaaggc gctcgaatat ctctggcgca ccggccggct cgccgtctgt cggcgggacg
     3541 gcttccagaa ggtctacgat ctcgtcgagc gggtcatccc cgaggcgcac cgccatgacg
     3601 ggatcgacga ggcggcgctg gtcgactggg cctgccgggc cgccctcgac cggctgggct
     3661 tcgccacctc gggcgagatc gccgccttct ggggcatcgt cacgccggcc gaggcccgaa
     3721 cctggtgcgc ggctgccgcc catggcgccg tcatcacctc gatcgaatcg gccgacggct
     3781 cggcgccgag gaaggtgctg gccgatgcgg cgctgctgga tctccacagc gacgatctcg
     3841 cacccccgcc ccgcatccgc gtgctcagcc ccttcgaccc cctggtccgt gatcgtgacc
     3901 gggcgcttcg cctcttcgac ttcgactacc ggatcgaggt cttcgtgccc gaggccaagc
     3961 ggcggtgggg ctactacgtc ttcccgctgc tcgagggcga ccgcttcgtc ggccgcatcg
     4021 acatgcgggc cgagcgcggc accggcacgc tcgccgtccg ccggctctgg tgggagccgg
     4081 gcatccgcgc gacccgcggc cggctgggcc ggctggaggc cgagctcgcg cgcgtggcgc
     4141 ggttcgccgg ctgcgaccgg gtgcggttcg acgagcatcc gggcgcgagc tgagcccggg
     4201 cctcagccct cgaagaaccg cgcactcttc tcgtcgagct cgcgcagggc ggcgtcgagc
     4261 gtcgcccggt cggcgccgtt ggtcacggcg tcgaggaagg gctggtagac ctcctccggc
     4321 acgccgctga agaggccgtc gacgatggcg ctgtcgacga ggcgggcgtt gagctcatcg
     4381 agcgcgaagc cgtggaccgt ggcgagcacg ccgatatcga cggcgaggcg gaagacgtag
     4441 gcggtccgtg ggtcgtgctg caccaggatg gcgttcgccg tgtcaccgtt ggccgtcttc
     4501 aggtagtcga gcgcggcctc gaggttggcc cgcgcggtgt cgccgcgctc tggcaagggc
     4561 gggatcgtca ggccgagcgt ctgggcgaag ccggacgcgg cccgccagat gtcgagcggc
     4621 tcggcttcgc ccgacagcat gtagcgcagc gtgccgacgg cgagcatgcc gcccagcttc
     4681 caggcctggc cgccggccgc agggtcgcgc gcgtccgtcg acggcgacgg acgctccgcc
     4741 ctgatcgcat cgagcttcgg ccgcagcgcc gtctcgacga acgcggcgtc gcgcttggtc
     4801 agctcctcca ggtccaccgc tgcccgctcg tccaggccga gggtcaccag cacctcggtt
     4861 cgcagcgcga gccgggcgcg ctcggtgacg tcgacacccc cgtccgcggc aatgacggca
     4921 tcgagatcga ccagggctgc cgccgggtcg cccagctcga ccgcgagatt cgcccgggcg
     4981 aggcggtagt cttccaggcg ccagtcgtct tccgccagtc cctcgatcgc cgccgtgtag
     5041 tcgtcgcgcg cctcggccgc gtggccgagc gcgcggtgcc gctcggcacg gtgggccagg
     5101 ttgtcagatg acggatcgag cccgaccgcg gcctcggcgt ccgccagggc ttccgccggg
     5161 cggcccagcg cgacatgcgc cccggagcgc tggagcagcg catccgcccg caagtagtcg
     5221 ttggtctcga gcgccacccg ccggtcggcg tcggcgaacc gggccacatc gtcgccgagc
     5281 tcggcatggg cctcggcgcg caaggccagg tatcccgggt tgtcgggctc gcgggccagg
     5341 aggcggttgg tgacctcgag cgccgtttcg ggatcgagga agatcgtcca ccagtcgttg
     5401 cgcgcgaggg ccagccaggt cgcatccgcc tcctcgatcc ggccgagtcc ggtgagcgcc
     5461 tcgatgcgcg cccagtagac gtcctcgagg gtcgagttcc aggccgtcgt ctggccgtgg
     5521 tcctccattt ccgccaaagc gaggttgaag ccgaagaggg cgaggcggta ctcgcccgcc
     5581 gccagcatct cgtaggcggt caccgcctgc acgtcgtagg tgtcggcggt ctgggttcgg
     5641 ctggcgttga aggtgatttc agccgtcacg tcgcggctga gcaaagcgag cacgcgatcg
     5701 cgcgccgtcg cggcggcggc ttcgtcgccc ggcgcgtggc tttcggccga tttcccgaac
     5761 cactgccagg ccgcttcggg gttgggcagc acgccccggc cgttctcgta ggcgaggccg
     5821 aggttgaact gggcccaggg gtcgccggcg agcgccgccc ggcggaacca tttgacggcc
     5881 tcgccgtcgt cggcgccggc gatccggcgg tcgtagtgca ggcggccgag ctcgttcgcc
     5941 gccggcgcat ggccctgctc ggccgagagt cggaagagcc gggcggcctc gtcgatgtcg
     6001 accgcgacgc cgcggccatc gagcaggtgg cgcgccaggt tgtgctggcc gagcctgtgg
     6061 ccctgcgcgg ccgagcgccg gaaccagagg accgcctcgg catcgttccg cgccacgccg
     6121 tggccctggt cgtagagggc gccgagattg ttctgggccc gggcattgcc gagctcggcg
     6181 atcggccgaa aaaggcgata ggcggtggcg tgatcgccgc ggttgtaggc ggcgaggccc
     6241 tcgtcgatcg ccgcctcctg cgccgcggcg atgctctccg gtgtcggcgc cacgcccgcc
     6301 gcgcctgcgc cgccggtcga ggcggcgccc tcgcgaccgc ggccctcgag gcgccggccg
     6361 tcg
//
LOCUS       DAOTFI010000258         6361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14500_length_6361_cov_6.801935, whole genome shotgun sequence.
ACCESSION   DAOTFI010000258 DAOTFI010000000
VERSION     DAOTFI010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14500_length_6361_cov_6.801935"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="P5558_20285"
     CDS             complement(<1..441)
                     /locus_tag="P5558_20285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY26719.1"
                     /translation="MRVLVVDDDPVSTSLLEAVLGREDCIVESTDMGAEALELARRYE
                     FDAVILDLQLPDLSGSQVLRRLRASGSVVPVLILSSLDDRAQKIQALVDGADDYLTKP
                     FDKHELLARLRVAVRRAKGHATSQITVGRLTLDLDARTVSVDDVP"
     gene            656..1099
                     /gene="mscL"
                     /locus_tag="P5558_20290"
     CDS             656..1099
                     /gene="mscL"
                     /locus_tag="P5558_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRY26720.1"
                     /translation="MLKEFREFALKGNVLDMAVGIIIGAAFTTVVSSLVDDVLMPPIG
                     VMMGGIDFSDYYLQLTNMDQDYPSLQAARDAGVTVIGYGSFLNAVIKFVIVAIALFII
                     VKNINRVKKAMEHEEERKADVAPPPRQEVLLAEIRDALLERPSRG"
     gene            complement(1129..2250)
                     /locus_tag="P5558_20295"
     CDS             complement(1129..2250)
                     /locus_tag="P5558_20295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY26721.1"
                     /translation="MTDTTFASAHRTLVTGFFGPSDPAWPAVLRMMPHDLYHLPDYAR
                     VEAARMGAEALGFWAQRDDRAFFVPVLLRDCPPLGGSGDGRRDAVSPYGYPGPIATPA
                     GCADPVFLRESLAALCGGLRALRACSLFLRSHPLLDPLPASLSMPGITHSTSPTVAVD
                     LTLPVEKLWSGTRRGHQSTINRCQRLGQTATVVAFDEGLPHLLPIYAAMLERTAADRQ
                     YHFDATYFEGLSRLAANLHIALVRVDSGEIAAACLLFECDGIVQAHLGGSDSRFIRQS
                     PFSLLIHHARLWARARGNRVLHLGGGVGGGDDAVMRFKTGFSSLTRPYRTLRAVLDAE
                     ACALLTAARAAELGTSPSELELLGYFPAYRTTGASPDRH"
     gene            complement(2247..3164)
                     /locus_tag="P5558_20300"
     CDS             complement(2247..3164)
                     /locus_tag="P5558_20300"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY26722.1"
                     /translation="MSSQPEYNDDDRSDAPACFELNDADVAFYELHGWYISPVVLPEA
                     LLDAAAAGAERFYAGERDRALPAQGAFKDWRPGEGAPIRNNEFVSLQNDAIRALVQAP
                     PIGAIAARLARTSSIRLLDDQLVYKAPAAPDAAEPGRATAVGWHADHAYWGTCSSDNM
                     LTAWIPFHDVGPDRSPLVVIDGSHRWGDLEHTRFFNDPDLDAVEARFRAQGHAVERVP
                     ITLKKGQISFHHCWALHGSLPNRSPLPRLALAVHLQDAANRYRRYLDPQGRPVRIADE
                     LLCRRLPNGEPDFSDPVPFPTLWPIDARS"
     gene            complement(3451..4542)
                     /locus_tag="P5558_20305"
     CDS             complement(3451..4542)
                     /locus_tag="P5558_20305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY26723.1"
                     /translation="MRHDDPLWLAVLARLRHDIHHHPAYLRAEARWLGGRPEAFLHVE
                     GEALWFAPCIVRRAQRPGVEAVGRTLDVVSPYGYPGPIGSPEHAAPGFCERAFDAFLA
                     ALRQRGAVSAFLRNHPCLDALPPVDESPLLTERAWKTVLIDLDGDLEAIWSRVSRRRR
                     QQINKCKRVGLQVRRVALRDGLPALMGIYDETMDRVGANADARFDAAYFHRLAPLAGH
                     LEVWLVERRETPIAATLVSRRCDIIQTPVGGTCEAALADSPDSLLCWELITWGHATGA
                     GAVHLGGGVGGRDDGVLRFKTSFGPGRARFRTVRAVLDPDRYAALTRARAASLGVAVQ
                     ALEQTGFFPAYRAELPGDARAAAPRAAGR"
     gene            complement(4753..5610)
                     /locus_tag="P5558_20310"
     CDS             complement(4753..5610)
                     /locus_tag="P5558_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26724.1"
                     /translation="MGLTGADWQEALLPHLDEAELAAWERARGRRVVELAGRHWAAIA
                     PGFYQPLHVLARIRANEIRRPQPLAWGWRAVLDPVDRAHANASLPVHVLPDLPGYTLE
                     RLQPRRRQQIRKALREVDVVALDDPSAVIDQGFALAMQAQARNPDVTVAGRDEFGRWV
                     EAFMAPRRGLVLGCLRHDRLLALCFGYAVGGVNYEHMTYVGEAGLPHNLALCLFHLKV
                     LIAQRTSGVTTIVNGLHARENPKLCEFKTRQGLVIEHVPTRLAIQPLAGFALRRWRPD
                     QYYRLTGAA"
     gene            complement(5601..6266)
                     /locus_tag="P5558_20315"
     CDS             complement(5601..6266)
                     /locus_tag="P5558_20315"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY26725.1"
                     /translation="MTDAAFVRTPDSNFADLADFPYPAHELDLGADAGGLTMRYVDEG
                     PRDAPVALMVHGMPTWSYLYRSIIPPMLAAGYRCVAPDHIGFGRSDKVTDPAWYDIAR
                     HTANLTRLIETLDLRDVTIFVQDWGGPTGLAQVAGRNLLSWEVRFEHDVTYVREIGPA
                     LDCWILWRTLRGLGDTSATVPTGGKDVSTFMGNPGSHSEGEADAIETTRDARSLERTG
                     AWG"
BASE COUNT         1042 a         2078 c         2293 g          948 t
ORIGIN      
        1 cgggacgtca tccaccgaga ccgtgcgggc gtccaggtcc agggtcagcc ggccgaccgt
       61 gatctgcgac gtggcgtggc ctttcgcccg ccgcaccgcg acccgcaggc gggccaggag
      121 ctcgtgcttg tcgaagggct tggtcaggta gtcgtccgcg ccgtcgacca gcgcctggat
      181 cttctgcgcc cggtcgtcga ggctcgaaag gatgagaacg ggcaccaccg agcccgatgc
      241 gcgcaggcgg cgcagcacct ggctgccgct gagatcgggc aactgcaggt cgaggatcac
      301 ggcgtcgaac tcgtagcggc gggcgagctc caacgcctcg gcacccatat ccgtgctttc
      361 gacgatgcag tcctcccgcc cgaggacggc ttccaagagc gaggtggaaa ccgggtcgtc
      421 gtccacaacg agcacgcgca tcagcctctc cagtcaagtc ggtttcatgc ccgacacaac
      481 cttagcgtga ttttttggtt aatccatccc ttgttgcgcg atttttggca cgacccttcg
      541 ccggatgggc cggcccggag ccaatgcgct gtcgcccaaa tcccctcgac cagtgcgagc
      601 agcgctggta acgcggatgg acggcggctc cgccttgccc tggagaagac atcgaatgct
      661 caaggagttc agagagttcg ccctgaaggg caatgtgctc gacatggctg tcggcatcat
      721 tatcggcgcc gcgttcacga ccgtcgtgag ctcgctcgtc gacgacgtgc tgatgccgcc
      781 gatcggcgtc atgatgggcg gcatcgactt cagcgactac tacttgcagc tgaccaacat
      841 ggatcaggac tacccgtcgc tgcaggcggc gcgcgatgcc ggggtcacgg tcatcggcta
      901 cggcagcttc ctcaacgcgg tgatcaagtt cgtgatcgtc gccatcgcgc tcttcatcat
      961 cgtcaagaac atcaaccgcg tgaagaaggc gatggagcac gaggaggagc ggaaggccga
     1021 tgtggcgccg ccgccccgcc aggaggtgct gctcgcggag atccgcgacg ccctgctcga
     1081 gcgtccgagc agaggctgag gccggtcgtg ccgccggaac gtgcgctgtc agtgtcggtc
     1141 gggcgatgcg ccagtggtcc gataggccgg aaaatagccc agtagctcga gctcgctcgg
     1201 ggacgtgccg agctcggctg cgcgggccgc ggtgagcagc gcgcaggctt ccgcgtcgag
     1261 gacggcacgg agcgtgcggt agggccgcgt caggctcgag aagccggtct tgaagcgcat
     1321 caccgcatcg tcgccgccgc cgacgccgcc gcccagatgc agcacgcgat tgccgcgcgc
     1381 cctggcccag aggcgggcat gatggatcag gagactgaac ggcgactggc ggatgaagcg
     1441 gctgtccgag ccgccgagat gggcctgcac gatgccatcg cattcgaaga ggaggcaggc
     1501 ggccgcgatc tcgcccgaat cgacccggac caaggcaatg tgcaggttgg cggccagccg
     1561 gctgagcccc tcgaaatagg ttgcatcgaa atgatactgc cgatccgccg ccgtccgctc
     1621 cagcatggcg gcatagatcg gcaggaggtg cggcaggccc tcgtcgaagg cgacgacggt
     1681 cgccgtctgg ccgagcctct ggcacctgtt gatggtgctc tgatggcctc gccgcgtgcc
     1741 ggaccagagc ttctcgaccg gcagcgtcag atcgaccgcg acggtcgggc tcgtggagtg
     1801 ggtgatgccg ggcatggaca ggctcgccgg caagggatcg agcagcggat gggagcgcag
     1861 gaagagcgag caggctcgca atgcccgcag gccgccgcag agcgcggcca gcgattcgcg
     1921 gaggaagacg ggatcggcgc accccgcggg ggtggcgatc ggcccggggt agccataagg
     1981 cgatacggca tccctgcggc cgtcgccgct gccgccgagc ggtgggcagt cgcgcagcag
     2041 gaccggcacg aagaaggcgc gatcatctct ctgtgcccag aagccgagcg cctcggcacc
     2101 catgcgcgcg gcctcgaccc tggcgtagtc gggcagatgg tagagatcgt gcggcatcat
     2161 gcggagcact gccggccagg ctggatcgga agggccgaag aagcccgtga ccagagtccg
     2221 gtgggcgctg gcaaaggtcg tgtcggtcac gatcttgcgt cgatcggcca gagtgtgggg
     2281 aatggcaccg gatcgctgaa atccggctca ccgttcggca ggcgtcggca gagcagctcg
     2341 tcggcgatcc gcaccggcct gccttgtggg tcgaggtagc ggcgatagcg gttggccgcg
     2401 tcctgcagat gcacggccag ggcgagacgc ggcaggggcg accggttcgg caggctgccg
     2461 tgcagcgccc agcagtgatg gaagctgatc tgccccttct tcagcgtgat cggcacgcgc
     2521 tccaccgcat ggccctgcgc gcgaaagcgc gcctcgaccg catcgaggtc cggatcgttg
     2581 aaaaagcgcg tgtgctcgag gtcgccccag cggtggctgc cgtcgatgac gacgagcggg
     2641 ctgcggtccg gcccgacatc gtggaacggg atccaggcgg tcagcatgtt gtcggagctg
     2701 caggttcccc agtaggcgtg atcggcatgc cagccgaccg ccgtggcgcg acccggctcg
     2761 gccgcatcgg gcgcggcggg cgccttgtag acgagttggt cgtcgagcag gcgaatgctc
     2821 gaagtgcggg cgagccgggc ggcgatcgcg ccgatcggcg gtgcctggac gagggcgcga
     2881 atggcgtcgt tctgcaggga cacgaactcg ttgttgcgga tcggggcacc ctcacccggg
     2941 cgccagtcct tgaacgcccc ctgcgccggc agcgcccggt cgcgctcgcc ggcgtagaag
     3001 cgctcggcac cggcggcagc tgcatcgagc agggcctcgg gcaggaccac gggcgagatg
     3061 taccagccat gcagctcgta gaaggcgaca tctgcgtcgt tcagctcgaa gcacgctggc
     3121 gcatcggacc gatcgtcgtc gttgtactct ggctggctgg acatcggcgt ttctttcgag
     3181 gacggaggct cggctgcggc agacgacagg ccggaaggac gatcgaactc attcagtggc
     3241 gtaacgactt ccagcaccgg aaagaactag cagctggagc ccgaacgtca tctgtcggat
     3301 tttcttacat ttacttgcaa cctcgaaggc ctggcgcgca gcttcgcggc attggccggc
     3361 caatgccgtg cagtcgggcc gctgcagcac cggcggctgc ctcccggctc gcgagatcgt
     3421 gtccgggaag gtgcgccgcg tcggtcccgg tcatcgcccg gccgccctgg gagctgcggc
     3481 cctagcatca ccgggcagct cggcccgata ggcagggaag aagccagtct gctccagggc
     3541 ctggacggcg acccctagcg aagccgcccg ggcccgggtg agtgctgcat agcggtccgg
     3601 atcgagcacg gcgcgcacgg tgcggaagcg ggcccggccg ggaccgaagc tcgtcttgaa
     3661 gcgcagcacg ccgtcgtccc ggccgccgac gccgccgccc agatgaacgg ccccggcccc
     3721 cgtcgcatga ccccaggtga tcagctccca gcagagaagc gagtcgggcg aatccgccaa
     3781 ggcagcttcg cacgtgccac cgaccggcgt ctggatgatg tcgcaacggc ggctgaccag
     3841 ggtcgccgcg atcggcgtct cccgtcgctc gacgagccag acctccaggt gaccagcgag
     3901 cggcgccagc ctgtggaaat aggcggcatc gaaacgcgcg tccgcattgg caccgacccg
     3961 atccatggtc tcgtcgtaaa tgcccatgag agccggcagg ccgtcccgta acgccacacg
     4021 acgcacctgc aagccgacac gtttgcactt gttgatctgt tggcgccgcc gtcggctgac
     4081 tcgcgaccag attgcctcca ggtcgccgtc gagatcgatc agcaccgttt tccaggcgcg
     4141 ctcggtcagg agcggcgact cgtcgaccgg cggcagggcg tcgaggcagg gatggttgcg
     4201 caggaaggcg ctgacggcgc cgcgctgccg cagcgccgcg aggaacgcgt cgaaggcgcg
     4261 ctcgcagaag cccggcgcgg catgctcggg cgaaccgatc gggccgggat agccataggg
     4321 cgagacgaca tcgagggtcc gaccgacggc ttccacaccg ggccgctgcg cgcgccggac
     4381 gatgcagggg gcgaaccaga gcgcctcgcc ctcgacatgg agaaaggctt cgggtcgacc
     4441 gccgagccac cgggcctcgg cgcgaagata agccggatgg tgatggatat cgtggcggag
     4501 ccgcgccagg acggcgagcc agagtgggtc gtcgtggcgc aaccatcggc cacgcggttc
     4561 gcttttcacc gccgggtcga tgctccgcac tatgcctcgc tccgtgaccg ccgttcgcgt
     4621 gcacggcaat ctgccatgcc ggcggttcgg aattcttaag acacagcgat tcttgcccgt
     4681 gaccgggcag cttacaccct cggctacgcc agccgttaac ctttcttcgg cggcggcgcg
     4741 gtgcagtgcc gttcaggcgg cgccggtcag tcgataatac tggtccggcc gccagcgacg
     4801 cagcgcgaag ccggcgagcg gctggatggc gaggcgggtg ggcacgtgct cgatgaccag
     4861 gccctggcgg gtcttgaact cgcaaagttt gggattttcg cgagcgtgca ggccattgac
     4921 gatcgtcgtg acgccgctcg tgcgctgggc gatcagcacc ttgagatgga agaggcagag
     4981 ggcgaggttg tgcggcaggc ccgcctcgcc cacataggtc atgtgctcgt agttcacgcc
     5041 gccgaccgca tagccgaagc acaaggcgag aagccggtcg tggcgcaggc agccgagcac
     5101 cagcccgcgg cgcggcgcca tgaaggcctc gacccagcgg ccgaattcgt cgcgcccggc
     5161 cacggtgacg tccgggttgc gggcctgcgc ctgcatcgcc agggcaaagc cctggtcgat
     5221 aacggccgaa ggatcgtcga gcgccaccac atcgacctca cgcagggcct ttcggatctg
     5281 ctggcgccgg cgcggctgga gtcgctcgag cgtgtagccc ggcagatcgg gaaggacgtg
     5341 caccggcagg ctcgcattgg cgtgcgcccg gtcgaccggg tcgagcacgg cccgccagcc
     5401 ccaggcgagc ggctgcgggc gccggatctc gttcgcccgg atgcgggcga ggacgtggag
     5461 cggctgatag aagccgggag cgatggcggc ccagtggcgg ccggccagct cgaccacgcg
     5521 cctgcccctg gcccgctccc aggcggcgag ctcggcctcg tcgagatgcg gcaggagcgc
     5581 ctcctgccag tcggcgccgg tcaaccccat gcgccggtcc gctcgagcga gcgcgcgtcg
     5641 cgcgttgtct cgatcgcgtc ggcctcgcct tcgctgtgcg atccggggtt gcccatgaag
     5701 gtcgagacgt ccttgccgcc ggtcggcacg gtggccgagg tgtcgcccag gccgcgcagc
     5761 gtgcgccaga ggatccagca atcgagtgcc gggccgatct cgcgcacata ggtgacgtcg
     5821 tgctcgaagc gcacctccca ggagagcagg ttgcgcccgg ccacctgggc gagcccggtg
     5881 gggccgcccc agtcctggac gaagatcgtg acgtcgcgca ggtcgagggt ctcgatcagg
     5941 cgggtgaggt tggcggtgtg gcgggcgatg tcgtaccacg ccggatcggt caccttgtcg
     6001 ctgcgcccga agccgatgtg gtcgggggcc acacagcggt agccggcggc cagcatcggc
     6061 gggatgatcg agcggtacag gtagctccag gtgggcatgc cgtgcaccat caacgcaacc
     6121 ggcgcgtcgc gtgggccctc gtcgacgtag cgcatcgtga gtcccccggc atcggccccg
     6181 aggtcgagct cgtgggccgg gtaggggaag tcggcgaggt cggcgaagtt cgagtcgggt
     6241 gtgcggacga acgcggcgtc ggtcatcggc acatcatcgc aaaccccggg tccttcgtga
     6301 gccggatgcg cagccggtgc gcattcggct cacgaagggt gggaggtcac caggacgcgc
     6361 c
//
LOCUS       DAOTFI010000259         6358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14510_length_6358_cov_7.220530, whole genome shotgun sequence.
ACCESSION   DAOTFI010000259 DAOTFI010000000
VERSION     DAOTFI010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14510_length_6358_cov_7.220530"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="P5558_20320"
     CDS             <1..241
                     /locus_tag="P5558_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRY26726.1"
                     /translation="IFANILADPLVAMAGDLRRHLASGGAAILSGLLDRQAPAVLAAH
                     RRQGMRSTGQVDRPPWAALVLRRSGAVRRAASKRN"
     gene            366..563
                     /gene="rpmI"
                     /locus_tag="P5558_20325"
     CDS             366..563
                     /gene="rpmI"
                     /locus_tag="P5558_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRY26727.1"
                     /translation="MTKLKTKRAAAKRFSFTGSGKIKRGHANHRHNFTSQPKKAKMRH
                     RKTGYVSAVDAPLVRLMLPHG"
     gene            618..977
                     /gene="rplT"
                     /locus_tag="P5558_20330"
     CDS             618..977
                     /gene="rplT"
                     /locus_tag="P5558_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRY26728.1"
                     /translation="MARVKRGVTSHARHKNVLEAAKGYRGRRKNCIKTARQAVEKAGQ
                     YAYRDRRVRKREFRALWIQRINAAAREQGMTYGQLMHGLKVAGVELDRKVLADIAVHD
                     AATFAGFVKQAGASLHA"
     gene            1103..2179
                     /gene="pheS"
                     /locus_tag="P5558_20335"
     CDS             1103..2179
                     /gene="pheS"
                     /locus_tag="P5558_20335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRY26729.1"
                     /translation="MATIDRVEGEMMAEIGSTPDLARLDELRVALLGKKGRVTDLVKG
                     VSSLPPAERPAAGQAFNELKRSLEAALLERRTALAEAALEARLLAERIDVTLPARPRD
                     GGRIHPVSQVTDELTAIFADMGFMVAEGPDIENDFHNFTALNFPPSHPAREMHDTFFL
                     EARQDGEPLLLRTHTSTIQVRHMMANKPPYRIIAPGRTYRCDSDATHTPMFHQVEGLV
                     IDRRTHMGHLKGCLIDFVRAYFERDDITARFRPSFFPFTEPSAEMDIGCRRTKEEIVV
                     GEGTDWLEILGCGMVHPNVLRAVGLDPAEWQGFAFGMGIDRIAMLKYGMPDLRPFFDA
                     DLAWLHHYGFVPLDLPTTFSGLSR"
     gene            2176..4572
                     /gene="pheT"
                     /locus_tag="P5558_20340"
     CDS             2176..4572
                     /gene="pheT"
                     /locus_tag="P5558_20340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRY26730.1"
                     /translation="MKFTLPWLKEHLETDASAAHIALTLTSLGLEVEAVHDRTSELSG
                     FSVAFVREAVQHPNADRLRLCTVETKHGVMQVVCGAPNARAGLHGIFAPEGSVIPATG
                     KVLGRSAIRGIESHGMLCSARELGIGDDHDGIIDLDGAPAVGTPASEALGIEGPVFEV
                     SLTPDRADCFGINGIARELAAAGLGQLRRRDFTPVPGQGRGPAIRLDFSAADAHACPL
                     FIGRLIRGVRNGPSPAWMQRRLQAIGLRPISALVDITNYVMFDLNRPLHVFDAARLEG
                     DVTVRFARAGETLLALDGREYALDDGMTVIADEAGPQGLGGVMGGEVSGVTGATTDVL
                     LEVALFDPIRTALTGRKLDLVSDARTRFERGLDPEMAVAGMEHATRLILELCGGEAAE
                     PVIAGAVPPGPAAIAFPVRELARLGGIQLERGEIEGHLRVLGFGLDGGPELYTVQPPS
                     WRHDVTSSACIVEELCRLHGYDKIPPVPVTRNEAVSAGVLTPEQRRRGHLRRTVAVLG
                     YTEAVTWSFTGENEARPFLEGEPVRLANPISSELSVMRPSLLPNLLLAAARNQDRKRE
                     DGALFELGARFTGGQPGEQVTALVGLRWGATGPRDWTGTRRPVDLFDAKADALAALAA
                     AGVKTEALQVTAGGPAWYHPGRSGTLGLGPNRLATFGELHPRVLEAFDLDGRAVAFEL
                     DLDALPRVRARPGRTRPPLEAWPYPPVDRDFAFLVDDGVSADQLLRAVRQADKKLIRE
                     VSLFDVYAGKGMPEGKKSLAVAVRLQSPDKTLTEADIEPVARKIVAAAEKQLGAELRG
                     "
     gene            4630..4914
                     /locus_tag="P5558_20345"
     CDS             4630..4914
                     /locus_tag="P5558_20345"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRY26731.1"
                     /translation="MRTMSAKEAKNHFGELLMEAQKAPVTIEKNGKPVAVLYSMEWHE
                     AAEEAKLDWLKRAVAEAVAEADRGELVEFDEEFIAELFSDGPSRFDDPRE"
     gene            4911..5231
                     /locus_tag="P5558_20350"
     CDS             4911..5231
                     /locus_tag="P5558_20350"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY26732.1"
                     /translation="MKRPRRALLVPAAARDLRRIRAWIAADSGVDRANVLLRALLDVV
                     ERIAELPALGTLRPSFGTGVRCFVRKPYLVFYRELDERVQVLRIIDGRRDLQIAWLED
                     KPVP"
     gene            5228..>6358
                     /gene="lepA"
                     /locus_tag="P5558_20355"
     CDS             5228..>6358
                     /gene="lepA"
                     /locus_tag="P5558_20355"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRY26733.1"
                     /translation="MTDLSHIRNFAIIAHIDHGKSTLADRLIQKCGGLADREMQAQVL
                     DSMDIERERGITIKAQTVRLRYTARNGRDYVFNLIDTPGHVDFAYEVSRSLRACEGSL
                     LLVDATQGVEAQTLANAYQAIEADHEIVPVLNKIDLPAAEPDRIRQQIEDIIGLEADN
                     ALMISAKTGLGVDTVLEAIVERLPPPQGEVAAALRALIVDSWYDAYLGVVTLIRVREG
                     VIRKGSRILMMGTRAAHQVDRVGVFTPKPLLVDELGPGEVGFITAAIKDIADARVGDT
                     ITEEKRQAAEALPGFRPSQPVVFCGMFPVDAADYEDLRDSLGKLRLNDASFEYEPESS
                     GALGLGFRCGFLGLLHLEIIQERLDREFDLDLVITAPSVVYRL"
BASE COUNT          962 a         2196 c         2223 g          977 t
ORIGIN      
        1 gatcttcgcc aacatcctgg ccgatccgct ggtggccatg gcgggcgatc tgcggcggca
       61 tctggcgtcg ggcggcgcgg cgattctgtc ggggctgctc gaccgccagg ccccggccgt
      121 gctggcggcg caccggcgcc aaggcatgcg gtccaccggg caggtggatc ggcctccctg
      181 ggccgccctc gtgctgcggc gcagtggcgc agtgcggcgt gccgcgtcga agcgcaattg
      241 atgggggccg cacagcggag gttgcgtctc ccgccggcct cggctatacg acgcgtcgca
      301 ttcgtcgccg accgcaccgg ctgcccgaga gggtggggcg gcgctgtcgt tcggagtttc
      361 ctggcatgac caagttgaag accaagcggg ccgctgccaa gcggttctcg ttcaccggca
      421 gtggcaagat caagcgtggt catgccaacc atcggcacaa cttcaccagc cagccgaaga
      481 aggcgaagat gcggcaccgc aagaccggct atgtcagcgc ggtggatgcg ccgctcgtcc
      541 ggctgatgct gccgcacggc tgacgtccgt cggcgtcgcc ggggcgcact ggaattttct
      601 gaccaaggat cgagacgatg gctcgggtaa agcgcggtgt aacctcccac gcccggcaca
      661 agaacgtgct ggaggcggcg aagggctatc gcggtcgtcg caagaactgc atcaagaccg
      721 cgcgtcaggc ggtcgagaag gccggccagt atgcctatcg cgaccggcgc gtccgcaagc
      781 gggagttccg cgccctctgg atccagcgca tcaacgcggc ggcgcgcgag cagggcatga
      841 cctacggcca gctcatgcac ggcctgaagg tcgccggcgt cgagctcgat cgcaaggtct
      901 tggccgacat tgccgtgcac gacgcggcga cgttcgcagg cttcgtgaag caggccgggg
      961 cctcccttca cgcctgaaat cggcgggacc gccagccggt ccctggcgat tcgatgaaga
     1021 ggggtcggca acggcccctt tttcgttttt ctacgccatt tccagcgggt ccacggccat
     1081 gacctcggag acgacatcgg tgctggcgac gatcgacagg gtcgaggggg agatgatggc
     1141 cgagatcggc agcacgcccg atctcgccag gctcgacgag ctgcgcgtgg cgctgctcgg
     1201 caagaagggt cgcgtgaccg acctcgtgaa aggcgtgagc agcctgccgc cggccgagcg
     1261 gccggcggcg gggcaggcct tcaacgagct gaagcggagc ctcgaggcgg cgctcctcga
     1321 gcggcgcacg gcgctcgccg aagcggcgct cgaggcgcgc ctgctggccg agcgaatcga
     1381 cgtcaccctg ccggcccggc cgcgtgacgg cgggcggatc cacccggtga gccaggtgac
     1441 cgacgagctc accgcgatct tcgccgacat gggcttcatg gtggccgagg ggccggacat
     1501 cgagaacgat ttccacaact tcacggcgct gaacttcccg ccgtcgcatc cggcccgcga
     1561 gatgcacgac acgttcttcc tggaggcgcg gcaggacggc gagccgctgc tgctgcgcac
     1621 ccacaccagc accatccagg tgcgccacat gatggccaac aagccgccct accggatcat
     1681 cgcccccggc cgcacctatc gctgcgacag cgacgcgacc cacacgccga tgttccatca
     1741 ggtcgagggc ctggtcatcg accgccggac ccacatgggc catctcaagg gctgcctgat
     1801 cgacttcgtc cgcgcctatt tcgagcgcga cgacatcacc gcccgcttcc gccccagctt
     1861 cttcccgttc accgagccgt cggccgagat ggacatcggc tgccgccgga cgaaggagga
     1921 gatcgtcgtg ggcgagggca cggactggct cgagatcctc ggctgcggga tggtccaccc
     1981 caacgtgctg cgcgccgtcg ggctcgaccc ggcggagtgg cagggcttcg ccttcggcat
     2041 ggggatcgac cgcatcgcca tgctgaaata cggcatgccc gaccttcggc cgttcttcga
     2101 tgccgatctc gcctggctgc accattacgg cttcgtgccg ctcgacctgc cgaccacctt
     2161 ctccgggctc tcgcgatgaa gttcaccctg ccctggctca aggagcatct cgagacggac
     2221 gcctcggccg cgcacatcgc cctgacgctg acctcgctcg ggctcgaggt cgaggccgtg
     2281 cacgaccgca cgagcgagct ttcgggcttc tccgtcgcct tcgtgcggga ggccgtgcag
     2341 caccccaacg ccgaccggct ccggctctgc accgtcgaga cgaagcatgg cgtcatgcag
     2401 gtcgtctgtg gcgccccgaa cgcccgggcc gggctccatg gcatcttcgc gcccgagggc
     2461 tcggtcatcc cggcgaccgg caaggtgctc gggcgcagcg ccattcgcgg catcgaaagc
     2521 catggcatgc tgtgctcggc gcgcgagctc ggcatcggcg acgaccatga cggcatcatc
     2581 gacctcgatg gcgcgccggc cgtcggcacg cctgccagcg aagcgctcgg catcgagggg
     2641 ccggtcttcg aggtgtcgct gaccccggac cgggccgact gcttcggcat caacggcatc
     2701 gcccgcgagc tggccgccgc cgggctcggc cagctcagga ggcgggattt cacgcccgtg
     2761 ccggggcagg gccgggggcc ggccatccgc ctcgactttt cggccgccga cgcgcatgcc
     2821 tgcccgctct tcatcggccg gctgatccgg ggcgtgcgca acgggccgag tccggcctgg
     2881 atgcagcggc ggctgcaagc cattgggctc aggccgattt cggcgctggt cgacatcacc
     2941 aattatgtca tgttcgacct caaccgaccg ctccacgtgt tcgacgccgc aaggctcgaa
     3001 ggcgacgtca ccgtccgttt cgccagagcg ggggaaaccc tgctggcgct ggacgggcgc
     3061 gagtacgccc tggacgacgg catgacggtg attgccgacg aggccggccc gcagggcctc
     3121 ggcggcgtca tgggcggcga ggtctcgggc gtcaccgggg ccaccaccga cgtgctgctg
     3181 gaggtcgctc tcttcgatcc gatccgcacg gcgctcaccg gtcgcaagct ggatctcgtg
     3241 agcgatgccc gcacccgttt cgagcgcggc ctcgacccgg agatggcggt cgccggcatg
     3301 gagcatgcca cgcggctcat cctcgagctc tgcggcggcg aggcggctga gccggtgatc
     3361 gccggtgccg tgccgcccgg ccctgcggcc atcgcctttc ccgtgcggga gctggcgcgg
     3421 ctcggcggca tccagctcga gcggggcgag atcgaggggc atcttcgcgt cctcggcttc
     3481 gggctcgatg gcgggcccga gctctacacc gtccagccgc cgagctggcg gcacgacgtc
     3541 accagctccg cctgcatcgt cgaggagctc tgtcggctgc acggctacga caagattccg
     3601 cctgtgcccg tcacccgcaa cgaggcggtg agcgctggcg tgctcacgcc cgagcagcgg
     3661 cggcgcggcc atttgcggcg gacggtggcg gtgctcggct ataccgaagc cgtgacctgg
     3721 tcgttcaccg gggagaacga ggcgcggccg ttcctcgagg gcgagccggt gcgcctcgcc
     3781 aacccgatct cgagcgagct ctcggtgatg cggccctcgc tcctgcccaa cctcctgctc
     3841 gcggccgccc gcaaccagga ccgcaagcgc gaggacggcg ccctcttcga gctgggtgcc
     3901 cggttcaccg gcggccagcc cggcgagcag gtgacggcgc tcgtggggtt gcgctggggg
     3961 gcgaccggcc cgcgggactg gaccggcacg cggcggccgg tcgatctctt cgatgccaag
     4021 gccgatgcgc tggcagcgct cgccgccgcc ggggtcaaga ccgaggcgct gcaggtcacg
     4081 gccggagggc cggcctggta ccatcccggc cgctcgggca cgctcggtct cgggccgaac
     4141 cggctcgcga ccttcgggga gctgcatccg cgcgtgctgg aagccttcga cctcgacggg
     4201 cgtgctgtcg ccttcgagct cgacctcgac gcgctgccca gggtgcgggc ccggccgggt
     4261 cgcacccggc cgccgctcga ggcctggccc taccctccgg tcgatcgcga cttcgcgttc
     4321 ctcgtcgacg acggggtcag cgccgaccag ttgctgcggg ccgtccgcca ggccgacaag
     4381 aagctgattc gcgaggtgtc gctgttcgac gtctatgccg gcaagggcat gcccgagggc
     4441 aagaagtcgc tggccgtcgc cgtccgtctg cagtccccgg acaagacgct gaccgaggcc
     4501 gacatcgagc cggtggcgag gaagatcgtg gcagcggccg agaagcagct cggcgccgag
     4561 ctgcgggggt gattgccgcg agagtccata atagctactt tggccaggat gggctttttg
     4621 gagacggcca tgcgcaccat gtcggccaag gaggccaaga accatttcgg cgagcttctg
     4681 atggaggcgc agaaggcgcc ggtgacaatc gagaagaacg gcaagccggt ggcggtgctc
     4741 tactcgatgg agtggcacga ggcggccgag gaggcgaagc tggactggct gaagcgggcg
     4801 gtggcggagg cggtcgccga ggcggatcgc ggcgagctcg tggagttcga cgaggaattc
     4861 attgcggagc tgttctccga cggtccctcg aggttcgatg acccgcgcga atgaagcgac
     4921 cccggcgagc gctgctcgtc cctgcggcgg cccgcgatct gcgtcgcatt cgcgcctgga
     4981 tcgcggccga ctccggcgtc gatcgggcca atgtcctgct ccgcgctctg ctcgacgtgg
     5041 tggagcggat cgccgagctg ccggcgctgg gaacgctccg tccgtcgttc ggcacgggcg
     5101 tgcggtgctt cgtgcgaaag ccctatctcg tcttctaccg cgagctcgat gagcgcgtgc
     5161 aagtattgcg tatcatagac ggccgtcgcg atctccagat cgcctggctc gaggacaagc
     5221 ctgttccatg accgacctct cgcatatccg caatttcgcg atcatcgcgc atatcgacca
     5281 cggcaagtcg accctggccg atcggctcat ccagaagtgt gggggtctcg ccgatcgcga
     5341 gatgcaggcg caggtgctcg attcgatgga tatcgagcgc gagcggggca tcaccatcaa
     5401 ggcgcagacc gtgcggctgc gctacacggc gaggaacggg cgcgactacg tcttcaacct
     5461 catcgacacg cccggccacg tcgacttcgc ctacgaggtc agccgctccc tgcgcgcctg
     5521 cgagggctcg ctgctgctgg tcgacgcgac ccagggcgtg gaggcgcaga ccctggccaa
     5581 cgcctatcag gcgatcgagg ccgaccacga gatcgtgccg gtgctcaaca agatcgacct
     5641 gccggccgcc gagcccgacc gcatcaggca gcagatcgag gacatcatcg gtctcgaggc
     5701 cgacaacgcg ctgatgatct cggccaagac cgggctcggc gtcgacacgg tgctggaagc
     5761 catcgtcgag cgcctgccgc cgccgcaggg cgaggtggcg gcagcgctgc gggcgctcat
     5821 cgtcgacagc tggtacgacg cctatctcgg ggtcgtcacc ctcatccggg tgcgcgaggg
     5881 cgtgatccgc aagggcagcc gcatcctgat gatgggcacg cgcgccgcgc accaggtcga
     5941 tcgggtcggc gtgttcacgc ccaagccgct gctcgtcgac gagctgggcc ccggcgaggt
     6001 cggcttcatc acggccgcca tcaaggacat cgccgacgcc cgggtgggcg acacgatcac
     6061 cgaggagaag cggcaggcgg ccgaggcgct gcccgggttc cgcccgtcgc agccggtcgt
     6121 cttctgcggc atgttcccgg tcgacgccgc cgactacgag gacctcaggg acagcctcgg
     6181 caagctccgg ctcaacgacg cgagcttcga gtacgagccg gagagcagcg gggcgctcgg
     6241 cctgggtttt cgctgcggct tcctcggttt attgcatctc gagatcatcc aggagcggct
     6301 ggaccgcgag ttcgacctcg acctcgtcat caccgccccc tcggtggtct accggctg
//
LOCUS       DAOTFI010000260         6340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14565_length_6340_cov_7.244232, whole genome shotgun sequence.
ACCESSION   DAOTFI010000260 DAOTFI010000000
VERSION     DAOTFI010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14565_length_6340_cov_7.244232"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /gene="glmS"
                     /locus_tag="P5558_20360"
     CDS             <1..1668
                     /gene="glmS"
                     /locus_tag="P5558_20360"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRY26734.1"
                     /translation="EAHPLGGSVGIGHTRWATHGAPTETNAHPHLAGGVAVVHNGIIE
                     NFAALRAELEAAGRVFTTQTDTEVVPHLVARELEAGSDPEAAARAAIRRLEGAFGLVL
                     LFQAEPGLLVAARRGSPLAIGYGTAGEAMYVGSDALALAPLTDRIQYLDEGDIAFVRI
                     AGAVVEDAGGAVVDRPIRQTALSGALIGKGNHRHFMEKEIFEQPAVLGDTLRSFAALG
                     DDGADHRIALPELPFDPAALERLTTIACGTAFYACMVAKYWFERTAGLAVECDIASEF
                     RYREPPMGPGTAGLFVSQSGETADTLAALRYVKAAGRPTLAVVNVPESTLAREADGAL
                     RTLAGPEIGVASTKAFTTQLATLACLALGCARARGRLDPAEESRLVKALDEVPALMLE
                     ALEHDDAVRRIALSILDARDVLYIGRGMMYPIALEGALKLKEISYIHAEGYAAGELKH
                     GPIALIDDTVPVVVVAPTDALFEKTASNLQEVVARGGRVILISDAAGVATLGEYATHS
                     LVLPEVDPFVAPLLYTVPIQLLAYHTAVLKGTDVDQPRNLAKSVTVE"
     gene            complement(1705..2790)
                     /gene="ugpC"
                     /locus_tag="P5558_20365"
     CDS             complement(1705..2790)
                     /gene="ugpC"
                     /locus_tag="P5558_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY26735.1"
                     /translation="MASVDVRKVEKFFGTVQVLHGVSIDIEDGEFVVLVGPSGCGKST
                     LLRMIAGLEEINRGEIAIGGRVVNTVPPKDRDIAMVFQNYALYPHMTVQQNMAFSLKL
                     AKAPKNVIEERVNRAADILGLREYLGRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIKELHQRLKTTAVYVTHDQIEAMTMADRIVVMQGGHVEQVGAP
                     LDLYDRPVNLFVAGFIGSPSMNFIQARIDRKNGSAVAVAADGSRLPLPADSGGQDGQE
                     IVYGIRPEHLTLVPEAEGQGAAPAEVIVVEPTGAEILVVTRFAGNEAQAVFKDRHPLR
                     PGDRIGLAPILESVHVFDKASGQRLGH"
     gene            2970..3854
                     /locus_tag="P5558_20370"
     CDS             2970..3854
                     /locus_tag="P5558_20370"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26736.1"
                     /translation="MRAEAPARGAPLAGGAALGILMMLIVQLIMPINDAMAKYLVASL
                     PVLQIAWARFFGNAVLLVPVVLWRRGLGALRTDRPGLQVARALAILAANLLFVTGVRV
                     VPLADALALIFVAPLAVTALSPWLLGEQVRRAEWVAVCLGFAGALVIIRPGSAGLDIA
                     ALLPLGAGLCFSLYLILTRKLAGTSPADVTLSITSLVAGGALSLLMPFVWVWPTLGET
                     LLLLAVGTCACTGHLLLTRALDFAPAASLAPFTYLSLVTATALGWFVFGDFPDGWTWI
                     GAAIVVLSGLVLWRFRQR"
     gene            3892..4206
                     /locus_tag="P5558_20375"
     CDS             3892..4206
                     /locus_tag="P5558_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26737.1"
                     /translation="MTPEQFQKSLADTTPPAGLAGPLEALWHAAKGDWRRAHTIVQKD
                     EADPTHAWVHAHLHREEGDLANAAYWYRRAGRPVAEGDIARERKAIAEVLLDAAGRGR
                     PV"
     gene            4261..4479
                     /locus_tag="P5558_20380"
     CDS             4261..4479
                     /locus_tag="P5558_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRY26738.1"
                     /translation="MSEHVYKVIELVGSSPESIEDATRNAIERASRTLKGLRWFEVVE
                     TRGHIEDGRIGHWQVMLKVGFTLNGDAG"
     gene            complement(4486..5355)
                     /locus_tag="P5558_20385"
     CDS             complement(4486..5355)
                     /locus_tag="P5558_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY26739.1"
                     /translation="MITDCHVNIWNDEHIRPHYRTQMARVREKDIPEKADAETIMAAM
                     APVDRAIVFSLRYAETIGIDSDDEVTADAVRRFPEKLTGFAYADPRRPDCLERLRHAI
                     EGLGLVGVKYGPIYNGVPLDDPRLEPVYAYCEKNDLPLTMHMGTTYTRLHAAELGRPL
                     AVEGVALRHPDLKLVMAHMGHPWFEECIAVIRKQPNVYAEISAIYYRRWQFYNVMLAI
                     EEYQVTDKVFFGSDFPFSTPGEGIELTRAVRQIGGTGGMPRVALETVERIITSDPFRH
                     WWHGGPEAAKPRA"
     gene            complement(5363..>6340)
                     /locus_tag="P5558_20390"
     CDS             complement(5363..>6340)
                     /locus_tag="P5558_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY26740.1"
                     /translation="GIIGGEIVLLGLIALPQMLRLGYDQKLAIGTICAGGSLGTMIPP
                     SIVLIVYGLTAGVSIGDLFLGGVLPGLLLAGFYITYIITRSVLNPALAPQLPADQLDI
                     PLTEKLRLLKGLILPILIIVWVLGSIYGGIASVTEAAGVGAVAALLAAAVRGEFSLAL
                     LWEAMQRTMSTVGIIIWLTLGANAFIGIYNIMGGTRYLSGAIAGLPLEPWQIILVMMF
                     ILFVLGMVLDWIGITLLTMPIFVPVIIDLGYDPVWFGILFCMNMQCSFLTPPFGPAAF
                     YLKSVAPPHIQLQTIFGALLPFIGMQILALLFLLVFPQLALWLPSLARG"
BASE COUNT          944 a         2253 c         2168 g          975 t
ORIGIN      
        1 gaggcgcatc cgctcggcgg cagcgtgggc atcggccata cccgctgggc gacccatggt
       61 gcgcccaccg agaccaacgc ccatccgcat ctggcgggcg gcgtggcggt cgtccacaac
      121 ggcatcatcg agaacttcgc ggcgctcagg gccgagctcg aggccgccgg ccgggtcttc
      181 acgacccaga ccgacaccga ggtggtgccg catctggtgg cccgggagct cgaggccggc
      241 agcgatcccg aggcggcggc gcgcgccgcg atccgccggc tcgagggcgc cttcggcctc
      301 gtcctcctct tccaggccga gcccggcctc ctggtcgcgg cgcgccgcgg cagcccgctc
      361 gcgatcggct acggcacggc cggcgaggcc atgtatgtcg gttccgacgc gctcgccttg
      421 gcaccgctca ccgaccgcat ccagtatctc gacgagggcg acatcgcctt cgtccgcatc
      481 gccggcgcgg tcgtcgagga tgccggcggt gccgtggtcg accggccgat ccgccagacc
      541 gccctctctg gcgccctgat cggcaagggc aatcaccggc acttcatgga gaaggagatc
      601 ttcgagcagc cggcggtgct cggcgacacg ctccgctcct ttgccgcgct gggcgacgac
      661 ggcgccgacc accggatcgc cctgcccgag ctgcccttcg acccggcggc cctggagcgg
      721 ctcaccacca tcgcctgcgg gaccgccttc tatgcctgca tggtcgccaa gtactggttc
      781 gagcgcacgg ccgggctcgc cgtcgagtgc gacatcgcct cggagttccg ctaccgcgag
      841 ccgcccatgg ggccgggcac ggccgggctc ttcgtctcgc agtcgggcga gaccgccgac
      901 acgctcgccg ccctgcgcta cgtcaaggcg gcgggccggc cgacgctcgc cgtcgtcaac
      961 gtgccggaga gcacgctcgc ccgcgaggcg gacggcgccc tgcgcacgct cgccgggccg
     1021 gagatcggcg tcgcgtcgac caaggccttc accacccagc tcgccacgct cgcctgcctg
     1081 gcgctcggct gcgccagggc gcgcggccgg ctcgacccgg ccgaggaatc acggctggtg
     1141 aaggcgctcg acgaggtgcc ggcgctgatg ctcgaggcgc tcgagcacga cgacgccgtc
     1201 cggcgcatag cgctcagcat tctcgatgcg cgcgacgtgc tctatatcgg tcgcggcatg
     1261 atgtacccga tcgcgctgga gggtgcgctg aagctgaagg agatcagcta catccatgcc
     1321 gagggctatg ccgcggggga gctcaagcac gggccgatcg cgctcatcga cgacaccgtg
     1381 ccggtggtgg tcgtcgcgcc gacggacgcc ctcttcgaga agaccgcgag caacctccag
     1441 gaggtcgtgg cccgcggcgg ccgggtgatc ctgatcagcg atgcggccgg cgtggccacc
     1501 ctcggcgagt acgccacgca cagcctcgtc ctgcccgagg tcgacccctt cgtggcgccg
     1561 ctgctctaca ccgtgccgat ccagctcctc gcctaccaca cggcggtgct caagggcacc
     1621 gacgtcgacc agccgcgcaa cctcgccaag tccgtcacgg tcgagtagcg ccggccttcg
     1681 tcggcgccgg ctcccttcgc tggctcagtg gccgaggcgc tggccggaag ccttgtcgaa
     1741 gacgtgcacc gattcgagga tcggggcgag gccgatgcgg tcgcccgggc ggagcggatg
     1801 gcggtccttg aagaccgcct gcgcctcgtt gccggcgaac cgggtgacga ccaggatctc
     1861 ggcaccagtc ggctcgacca cgatcacctc ggccggggcc gccccctggc cctcggcctc
     1921 gggcaccagg gtgagatgct ccgggcgaat gccgtagacg atctcctggc cgtcctggcc
     1981 gccggaatcg gccggcagcg gcaggcggct gccgtcggcg gcgaccgcca cggccgagcc
     2041 gttcttccgg tcgatccgcg cctggatgaa gttcatcgag ggcgagccga tgaagccggc
     2101 gacgaagagg ttgaccggcc ggtcgtagag gtcgagcggg gcgcccacct gctcgacatg
     2161 gccgccctgc atgacgacga tgcggtcggc catggtcatg gcctcgatct ggtcgtgggt
     2221 gacgtagacg gccgtggtct tgagccgctg gtgcagctcc ttgatctcgg tgcgcatctg
     2281 gacgcgcagc ttggcgtcga ggttggagag cggctcgtcg aagaggaaga cctgcgggtc
     2341 gcggacgatg gcccggccca tggcgacgcg ctgccgctgg ccgcccgaga gctgcctcgg
     2401 atagcggccg agatactcgc gcagccccag gatgtcggcg gcgcggttga cccgctcctc
     2461 gatcacgttc ttcggcgcct tggcgagctt gagcgagaac gccatgttct gctgcacggt
     2521 catgtgcggg tagagggcgt agttctggaa gaccatggcg atgtcgcgat ccttcggcgg
     2581 cacggtgttg accacccgcc cgccgatcgc gatctcgccg cggttgatct cctcgagccc
     2641 ggcgatcatc ctgagcaggg tcgacttgcc acagcccgag gggcccacga gcacgacgaa
     2701 ctcgccatcc tcgatatcga tcgacacgcc gtgcaagacc tgcaccgtgc cgaagaactt
     2761 ctccaccttg cgaacgtcga cggaagccat gtgccctttt cccgatcctg cctcggcgcc
     2821 gaccccgatg acctggcggg ccgaaccgtt tcccctgatg acgagcccgc tgcccctgcc
     2881 gaagacggga gggctattgc tctggtggta tcatgaccgc tttcgcaccc cagttcaatg
     2941 cgcgacaggc acttcccggc gaacgtttcg tgcgggctga ggcaccggca cggggtgccc
     3001 cgctcgcggg cggggccgcc ctcggcatcc tgatgatgct gatcgtccag ctcatcatgc
     3061 cgatcaacga cgccatggcc aagtacctgg tcgccagcct gccggtcctg cagatcgcct
     3121 gggcccggtt cttcggcaac gcggtgctgc tcgtgccggt cgtcctctgg cggcgcggcc
     3181 tcggcgcctt gcgcaccgac cggccgggcc tgcaggtggc ccgcgccctc gccatcctcg
     3241 ccgccaacct gctcttcgtc accggtgtca gggtggtgcc gctggccgac gccctggccc
     3301 tcatcttcgt cgccccgctc gccgtcaccg ccctctcgcc ctggctgctc ggcgagcagg
     3361 tcaggcgggc cgagtgggtc gcggtctgcc tcggcttcgc cggggcgctg gtcatcatcc
     3421 gccccggcag cgccggcctc gacatcgccg ccctcctgcc gctcggcgcc gggctctgct
     3481 tctcgctcta cctcatcctc acccgcaagc tggcgggcac gagcccggcc gacgtcacgc
     3541 tctccatcac ctcgctggtc gccggtggtg ccctcagcct gctcatgccc ttcgtctggg
     3601 tctggccgac cctgggcgag acgctgctgc tgctggcggt cggcacctgc gcctgcaccg
     3661 gccacctgct cctgacccgc gccctcgact tcgccccggc cgccagcctc gcccccttca
     3721 cctatctctc gctggtcacg gcaacggccc tcggctggtt cgtcttcggc gacttccccg
     3781 atggctggac ctggatcggt gccgccatcg tcgtgctcag cgggctcgtg ctctggcgct
     3841 ttcgccagcg ctgaccgctc ccctcttcaa ccctcgaccg gagatgcgac catgacgccc
     3901 gagcagttcc agaagagcct cgccgacacc accccgcccg ccggtctcgc cggcccgctc
     3961 gaggcgctct ggcacgccgc caaaggcgac tggcggcgtg cccacacgat cgtccaaaag
     4021 gacgaggcgg accccaccca cgcctgggtc catgcccacc tgcatcgcga ggagggcgac
     4081 ctcgccaacg ccgcctactg gtatcgccgg gccggccgcc cggtggccga aggcgacatc
     4141 gcccgggaac gcaaggcgat cgccgaggtt ctcctcgatg ccgcgggccg aggccgcccg
     4201 gtttgaagcg gtcgtccggc tcgggctaga ctggacccgt ccaacgaggg aggatcgtcc
     4261 atgagcgaac atgtctacaa ggtgatcgag ctcgtcggct cctcgccgga gagcatcgag
     4321 gacgccacga gaaatgccat cgagcgcgcc agccggaccc tgaagggcct gcgctggttc
     4381 gaggtcgtcg agacccgtgg ccacatcgag gacggcagga tcggccactg gcaggtgatg
     4441 ctgaaggtgg gcttcaccct gaacggcgac gccggctagc ctccctcagg cgcgcggctt
     4501 tgccgcctcg gggccgccgt gccaccagtg gcggaagggg tcgctggtga tgatccgctc
     4561 gaccgtctcc agggcgaccc gcggcatgcc gcccgtgccg ccgatctggc gcaccgcccg
     4621 cgtcagctcg atgccttcgc cgggcgtcga gaacgggaag tcgctgccga agaagacctt
     4681 gtccgtgacc tggtactcct cgatcgccag catgacgttg tagaactgcc agcggcgata
     4741 gtagatcgcc gagatctcgg cgtagacgtt gggctgcttc ctgatgacgg cgatgcactc
     4801 ctcgaaccag ggatggccca tatgcgccat caccagcttg aggtcggggt gccggagcgc
     4861 cacgccctcg accgccagcg gccggccgag ctcggcggcg tggagccgcg tgtaggtggt
     4921 gcccatgtgc atggtcagcg gcaggtcgtt cttctcgcaa taggcataga ccggctcgag
     4981 cctggggtcg tcgagcggca cgccgttgta gatcggcccg tacttgacgc cgacgaggcc
     5041 gagcccctcg atcgcgtggc ggagccgctc gaggcagtcc ggccggcgcg ggtcggcata
     5101 ggcgaagccg gtgagcttct ccggaaagcg gcgcaccgcg tcggccgtga cctcgtcatc
     5161 gctgtcgatg ccgatcgtct cggcgtagcg cagcgagaag acgatcgccc ggtcgaccgg
     5221 cgccatggcc gccatgatcg tttcggcatc cgccttctcg gggatgtcct tctcgcgcac
     5281 ccgggccatc tgcgtgcggt agtgcggccg gatgtgctcg tcgttccaga tgttgacgtg
     5341 gcagtcggtg atcatgacgc gctcaccccc gcgccaggct gggcagccag agggcgagct
     5401 gcgggaagac caaaaggaac agcagggcca ggatctgcat gccgatgaac ggcagcaacg
     5461 ccccgaagat cgtctgcagc tggatatgcg gcggggccac ggacttgaga tagaaggcgg
     5521 ccgggccgaa gggcggggtc aggaagctgc actgcatgtt catgcagaag agaatgccga
     5581 accagaccgg gtcgtagccg aggtcgatga tcaccggcac gaagatcggc atggtcagga
     5641 gcgtgatgcc gatccagtcg agcaccatgc cgagcacgaa gaggatgaac atcatcacga
     5701 ggatgatctg ccagggctcg agcggcagcc cggcgatggc cccgctcaga tagcgggtgc
     5761 cacccatgat gttgtagatg ccgatgaagg cgttggcgcc gagcgtcagc cagatgatga
     5821 tgccgaccgt cgacatcgtc cgctgcatcg cctcccagag aagggccagg ctgaactcgc
     5881 cgcgcaccgc cgccgccagc agggccgcca ccgcgccgac gccggccgcc tcggtgacgc
     5941 tggcgatgcc gccatagatg gagcccagga cccagacgat gatcaggatc ggcaggatca
     6001 ggcccttcag cagccggagc ttctcggtga gcgggatgtc gagctggtcc gccggcaatt
     6061 gcggcgccag cgccgggttc aggacggacc gggtgatgat gtaggtgatg tagaagccgg
     6121 ccagcagcag gcccggcagg acgccgccga ggaagagatc gccgatcgag acgccggcgg
     6181 tgaggccgta gacgatgagc acgatcgacg gcgggatcat cgtgccgagc gagccgccgg
     6241 cgcagatcgt gccgatggcg agcttctggt cgtagccgag ccgcagcatc tgcggcaggg
     6301 cgatcaggcc gagcagcacg atctcgccgc cgatgatgcc
//
LOCUS       DAOTFI010000261         6332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14583_length_6332_cov_6.696670, whole genome shotgun sequence.
ACCESSION   DAOTFI010000261 DAOTFI010000000
VERSION     DAOTFI010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14583_length_6332_cov_6.696670"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="P5558_20395"
     CDS             complement(<1..316)
                     /locus_tag="P5558_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26741.1"
                     /translation="MNVPRKLDLTSLSRRLALGSAIALLAMAGGAMAVEYHEAPALAE
                     QVAAGTLPPVAERLPADPEVVTPIESIGTYGGQLRYGLRGSSDHNNILRMVGPQGLVR
                     WSP"
     gene            495..1640
                     /locus_tag="P5558_20400"
     CDS             495..1640
                     /locus_tag="P5558_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26742.1"
                     /translation="MRIVRLRSFMARDRNRPRVVVAIDTDEGITGWGECYNHGPDRAL
                     PPLLEYLFGQIEGHDPRRIEFLILKLLQQARFPPGALGLAAISAIDHALWDISAKALN
                     VPVFMLLGGHARDRVRVYASLGGYHARDAEAGIAFIAEQNEKWGFTAFKFSPFRTDLH
                     SLRWGNVVRASADYFGRLREACPSEYEFAFDAHAKIFEPAQAVQLGNALAAYDPLFYE
                     EPIRPEHIPAWGRLRSELRCPLATGESLYSRFEFLNLLTARGADIIQPDICAVGGLLE
                     MRKIAALAEAHYVTVAPHNPMGPLATAVNLHFSAAQPNFKVLEYLLPADTSWVVDPYL
                     PKDGYLELRHDRPGWGVEIDEQALAKDDYVHWERKLPIRPDGSTGYC"
     gene            complement(1642..2859)
                     /locus_tag="P5558_20405"
     CDS             complement(1642..2859)
                     /locus_tag="P5558_20405"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26743.1"
                     /translation="MRITKIETLRVDSVGNMLWLGVSTDAGITGLGEAFRNAGATEAY
                     IHETLAPWLLGKDPLEINAHREAIGRRIGNRFMGTPSRSIEVRGNAAVDLALWDILGK
                     VLNVPIAQLFGGRVREEIRVYNTCAGPAYNTSPTVGWVSRLAEPGAAPLAPLDDLQAQ
                     LDDPGGLARSLMAEGITAMKIWPLDRFAAKSGGSEITAADLDAALAPVRAIRDAVGDA
                     MDIMIECHGMWRLPAAVRIARALEPFGIFWLEDPIEMHRLDDLARLKQATSLAIAGSE
                     NHGTAHWYAEAFARGLVDYAHFDIGWMGGLSEALDVAALARAHDRCIAPHDCVGPVML
                     AANMQLVFSQPHALVLETVRAYLRGFYAEVMQPAPVIEAGMARPLDGPGLGIELAPEL
                     WTRPDSRSRSSTL"
     gene            complement(2866..3810)
                     /locus_tag="P5558_20410"
     CDS             complement(2866..3810)
                     /locus_tag="P5558_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY26744.1"
                     /translation="MKTTAVTPADLAASVLAVPPLPLAADLSPSEPEMRRLVRHIEAG
                     GVSTILWGGNAQLQHWPASRYSEFLALAEATAGRASWVIPSVGPDYGKLMDEARSLAG
                     TRFPCAMLLPMAAHHTPAGVARGTRDFVQAAGKPAIVYIRQAGYVAPDALGAMVAAGE
                     VCAVKYAVDRPDLRDDPYLAALVAAVGSERIVSGAGEIVAVPHLDAFGLAGFTAGCVC
                     ITPRLSMEVLRLLKAGDQAAAAPALEAIRPLEGLRERLSPIRVLHEAVRLAGVAETGP
                     IYPLLSNLEAEHHPAILAALKPLMAAEQALHPATALAG"
     gene            4086..4937
                     /locus_tag="P5558_20415"
     CDS             4086..4937
                     /locus_tag="P5558_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26745.1"
                     /translation="MHEPLLRVDDLHVSFRTPRGVVNAVRGVSFEVGREKVGIVGESG
                     SGKSITGRAVMRLLPPAAEARANRLSFRDIDLLRASEKQMLDIRGGQIGLIMQDPKYS
                     LNPVVRVGDQIAEAYRLHRGGNRKAARASAMAMLEAVKIRDPERVYRLYPHEVSGGMG
                     QRIMIATMLLAEPDLLIADEPTSALDVTVRLEVLSILDELIETRGLGLMFISHDLNLV
                     RAFCDRVLIMYAGRIVEELPAAELEHARHPYTQGLLAALPRIHQRRERLAVLQRDPAW
                     LDGPTYQ"
     gene            4964..5773
                     /locus_tag="P5558_20420"
     CDS             4964..5773
                     /locus_tag="P5558_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26746.1"
                     /translation="MIAVDGLVVSFGDDDEVKRAVDGIGFQVREGETFGLVGESGCGK
                     STVLRVLAGLNPTYAGRLRIAGRDIGPKRDKALMKSVQMVFQDPYGSLHPRQTVNAQL
                     MEPLRIQGLDDAAGRVERALDAVGLGAGFRYRYPHQLSGGQRQRVAIARALILEPDVL
                     LLDEPTSALDVSVQAEVLNLLADLKAAGGLTYVLVSHDLGVIAHMCDRLAVMTEGRIV
                     EELDVQALVAGAARHPYTVTLLRASETYGEGGVPAAARAPDEAQLPEPGKT"
     gene            5770..>6332
                     /locus_tag="P5558_20425"
     CDS             5770..>6332
                     /locus_tag="P5558_20425"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26747.1"
                     /translation="MMRITDVQARVFIYKTSKMNDTDGHTHPGPERDARGAILTIACD
                     DGAEGHVIAPPSTVTEELLAEFVRPVLVGADPMRIEHLWRALYKWQRGSGGRLTDRVV
                     CAVELALWDLVGRKVGQPVWKLLGGYRDKILAYGSTMCGDDLANGLATPEDYGRYAEW
                     LVQKRGYKAVKLHTWMPPRSFQTGWPT"
BASE COUNT          941 a         2262 c         2203 g          926 t
ORIGIN      
        1 cggggctcca gcggacgagg ccctgcgggc cgaccatgcg caggatgttg ttgtggtccg
       61 agctgccgcg cagcccgtag cggagctggc cgccataggt gccgatgctc tcgatcggcg
      121 tcaccacctc cgggtcggcc ggcaggcgct ccgcgaccgg cggcagagtc ccggccgcca
      181 cctgctcggc gagcgccggc gcctcgtggt actcgaccgc catcgcgccg ccggccatgg
      241 ccaggagagc aatggcactg cccagcgcca gccggcgcga cagcgatgtc agatctagct
      301 ttcgcggaac gttcatccgc acgcctcccc tatgatcgat cgagcctcgg tgggcccgtg
      361 cctcggggtc gaggttcgtt gccccacccg gctttgtcaa accatttgcc gagggatgtc
      421 gcggtcgatc cggcgagttg aaacgcgacc gacaatgtgg ctctctcccg acctgttcgc
      481 cccagggagg cccgatgaga atcgttcgct tgagaagctt catggcacgc gaccgcaacc
      541 ggccgcgggt ggtcgtcgcc atcgacaccg acgagggcat caccggctgg ggcgaatgct
      601 acaaccacgg gcccgaccgg gcgctgccgc ccttgctcga atacctcttc ggccagatcg
      661 agggccacga cccgcgccgc atcgagttcc tgatcctcaa gctcctgcag caggcacgct
      721 tcccgcctgg ggcgctcggc ctcgccgcga tctccgccat cgaccacgcg ctttgggaca
      781 tctcggcgaa ggcgctgaac gtgcccgtct tcatgctgct cggcggccat gcccgcgacc
      841 gcgtgcgggt ctacgcctcg ctcggcggct atcacgccag ggacgcggaa gcgggcatcg
      901 cgttcatcgc ggagcagaac gagaagtggg gcttcaccgc cttcaagttc agcccgttcc
      961 gcaccgacct gcacagcctg cgctggggca acgtggtgcg cgcgagcgcc gactatttcg
     1021 gtcgcctcag ggaggcctgc ccgagcgagt acgagttcgc cttcgacgcg cacgccaaga
     1081 tcttcgagcc cgcgcaggcg gtccagctcg gcaacgccct cgccgcctat gatccgctct
     1141 tctacgagga gccgatccgc cccgagcaca tcccggcctg gggccggctg cggtccgagc
     1201 tgcgctgccc gctcgccacc ggcgaatcgc tctacagccg cttcgagttc ctgaacctgc
     1261 tgaccgcccg gggtgccgac atcatccagc ccgacatttg cgccgtcggc ggcctgctgg
     1321 agatgcgcaa gatcgcggcc ctggccgagg cgcattacgt gacggtggcg ccgcacaacc
     1381 cgatgggtcc cctggcgacc gcggtcaacc tgcacttctc cgccgcccag cccaacttca
     1441 aggtcctcga atacctgctg ccggcggaca cgagctgggt ggtcgatccc tacctgccga
     1501 aggacggcta tctcgagctg cgccacgacc ggccgggctg gggtgtcgag atcgacgagc
     1561 aggcgctggc caaggacgac tacgtccact gggagcgcaa gctgccgatc cgcccggacg
     1621 gctcgaccgg ctactgctga gctacagggt cgagctccgg ctccgggagt ccggccgcgt
     1681 ccacagctcc ggtgccagct cgatccccag ccccggcccg tcgagcgggc gcgccatgcc
     1741 cgcctcgatc accggcgccg gctgcatgac ctcggcatag aagccgcgca gataggcgcg
     1801 cacggtctcg aggacgaggg cgtgtggctg gctgaagacg agctgcatgt tggcggccag
     1861 catcaccggg ccgacgcagt cgtgcggggc gatgcagcgg tcgtgggccc gggcaagggc
     1921 cgcgacgtcc agcgcctcgg agaggccgcc catccagccg atgtcgaaat gcgcatagtc
     1981 gacgaggccg cgggcgaacg cctcggcgta ccagtgcgcc gtgccgtggt tctcgctgcc
     2041 ggcgatggcc agcgacgtcg cctgcttcag ccgcgccagg tcgtcgagcc ggtgcatctc
     2101 gatcgggtcc tccagccaga agatgccgaa gggctcgagt gcccgggcga tccgcacggc
     2161 tgccggcagg cgccacatgc cgtggcattc gatcatgatg tccatggcgt cgccgaccgc
     2221 atcgcggatg gcgcggacgg gggcgagggc cgcgtcgaga tcggcggcgg tgatctccga
     2281 gccgccgctt ttggcggcga agcggtcgag gggccagatc ttcatggccg tgatcccctc
     2341 ggccatgagc gagcgcgcca ggccgcccgg gtcgtcgagc tgcgcctgga gatcgtcgag
     2401 cggggccagc ggcgccgccc cgggctcggc cagccgcgac acccagccga cggtcgggct
     2461 cgtgttgtag gccgggccgg cgcaggtgtt gtagacgcgg atctcctcgc gcacccggcc
     2521 gccgaagagc tgggcgatgg gcacgttcag cactttgccg aggatgtccc agagggcgag
     2581 gtcgacggcc gcgttgccgc gcacctcgat cgagcgcgac ggcgtgccca tgaagcggtt
     2641 gccgatgcgc cgcccgatcg cctcgcgatg cgcgttgatc tcgagcgggt ccttgccgag
     2701 gagccagggg gccagggtct cgtggatata ggcctccgtc gccccggcat tgcggaaggc
     2761 ctcgccgagg ccggtgatgc cggcgtcggt cgacacaccc agccagagca tgttgccgac
     2821 ggagtcgacc cgcagcgtct cgattttggt gatccgcaag gtgcttcagc cggccagcgc
     2881 ggttgccggg tgcagcgcct gctcggccgc catcaacggc ttaagggctg ccaggatcgc
     2941 cggatggtgc tcggcctcga gattggagag cagcgggtag atcggcccgg tttccgccac
     3001 cccggcgagg cggaccgcct cgtgcagcac gcggatcggc gagaggcgct cgcgcagccc
     3061 ctcgagcggc cggatcgcct ccagcgccgg tgcggcggcg gcctgatcgc cggccttgag
     3121 cagccgcagc acctccatcg acagcctggg ggtgatgcag acgcagccgg cggtgaagcc
     3181 ggcgaggccg aaggcgtcga gatgcggcac ggcgacgatc tcgccggcgc ccgagacgat
     3241 gcgctcggac ccgaccgcgg cgaccagggc ggcgagatag gggtcgtcgc gcagatcggg
     3301 ccgatcgacc gcgtacttca ccgcgcagac ctcgcccgcg gcgaccatcg cccccagcgc
     3361 atccggtgcg acgtagccgg cctggcggat gtagacgatg gcgggcttgc cggcagcctg
     3421 gacgaagtcg cgcgtgccgc gggcgacacc cgccggcgtg tgatgcgccg ccatcggcag
     3481 cagcatggcg cagggaaagc gggtgccggc gaggctccgc gcctcgtcca tgagcttgcc
     3541 gtagtcgggc ccgaccgagg ggatcaccca gctcgcccgc cccgccgtcg cctcggcgag
     3601 ggcgaggaac tcgctgtagc ggctcgccgg ccagtgctgg agctgggcat tgccgcccca
     3661 gaggatggtg ctgacgccac cggcctcgat atggcggacg aggcgccgca tctcgggctc
     3721 gctgggcgaa aggtccgcgg cgagcggcaa gggcggcacc gccagcaccg aggcggcgag
     3781 atcggcaggg gtgacggcgg tggtcttcat gggctcgggt gctccctcat tgtcgtgccg
     3841 gcgcgtcgtg ccccggcacc atcggtcatt gggtggacgc tatgcgatcc cggcccggac
     3901 gccaaccatg cgcgggcctg tccggatggc catctcctcc ggcccaaccg gattgccggt
     3961 cgggtcgcct gctgatatcc gaagtccgga gccgtgaagt cgctgcgtcg tcactttgca
     4021 gcatgaacag gcagggtagc cctgcgcgac atcctcgagc cccccagcag ctgacagcag
     4081 gcgccatgca cgagcccctc cttcgggtcg acgatctgca cgtgagcttc cgcacgcccc
     4141 ggggcgtggt caacgcggtg cgcggcgtct ccttcgaggt cggccgcgag aaggtcggca
     4201 tcgtcggcga gtcgggctcc ggcaagagca tcaccggccg cgccgtgatg cgcctcctgc
     4261 cgccggccgc cgaagcccga gccaaccggc tgagctttcg cgacatcgat ctcctgcgcg
     4321 cctccgagaa gcagatgctg gacattcgtg gcgggcagat cggcctgatc atgcaggacc
     4381 cgaaatactc gctcaatccc gtggtccggg tcggcgacca gatcgccgag gcctatcgcc
     4441 tgcaccgggg cggcaaccgc aaggcggcgc gggcgagtgc gatggccatg ctcgaggcgg
     4501 tcaagatccg cgatcccgag cgtgtctacc ggctctaccc gcacgaggtc tcgggcggca
     4561 tgggccagcg catcatgatc gccaccatgc tgctcgccga gcccgacctc ctgatcgccg
     4621 acgagccgac ctcggccctg gacgtgacgg tgcgcctcga ggtgctctcg attctcgacg
     4681 agctgatcga gacgcggggc ctgggattga tgttcatcag ccacgacctc aatctcgtcc
     4741 gcgccttctg cgaccgggtg ctgatcatgt atgccggccg catcgtcgag gagctgccgg
     4801 ccgccgagct cgagcatgcc cggcatccct acacccaggg tctgctggcg gccttgcccc
     4861 gcatccatca gcggcgggag cggctggcgg tcttgcagcg cgaccccgcc tggctggacg
     4921 ggccgaccta ccaatgactc tgcgcgaacc cgggaccggg cccatgatcg ccgtggacgg
     4981 cctcgtcgtc agcttcggcg acgacgacga ggtcaagcgg gccgtggacg gcatcggctt
     5041 tcaggtgcgc gagggcgaga ccttcggcct cgtcggcgag tcgggctgcg gcaagtccac
     5101 cgtgctgcgc gtgctcgccg gcctcaaccc gacctatgcc ggcaggctgc gcatcgccgg
     5161 gcgggacatc gggccgaagc gcgacaaggc gctcatgaag agcgtgcaga tggtcttcca
     5221 ggacccctac gggtcgctcc atccgcgcca gacggtgaac gcccagctca tggagcccct
     5281 gcgcatccag ggactcgacg atgccgcggg tcgggtcgag cgggcgctcg atgccgtcgg
     5341 cctcggcgcc ggcttccgct accgctaccc gcaccagctc tccggcggcc agcgccagcg
     5401 ggtcgccatc gcgcgcgccc tcatcctcga gccggacgtg ctgctgctcg acgagcccac
     5461 ctccgcactc gacgtctcgg tccaggccga ggtgctcaac ctcctggccg acctcaaggc
     5521 ggcgggcggc ctcacctacg tgctcgtcag ccacgacctc ggcgtgatcg cccacatgtg
     5581 cgaccgcctc gccgtcatga cggaagggcg catcgtcgag gagctcgacg tccaggcgct
     5641 cgtcgcgggt gccgcccggc acccctacac ggtgaccctg ctccgggcca gcgagaccta
     5701 tggcgagggc ggcgtgccgg ctgccgcacg cgcgcccgac gaagcccaac tccctgaacc
     5761 cgggaagaca tgatgcggat caccgacgtc caggctcgcg tgttcatcta caaaaccagc
     5821 aagatgaacg acacggatgg ccacacccat cccggccccg agcgtgacgc caggggggcg
     5881 atcctgacga tcgcctgcga cgacggcgcc gagggccatg tcatcgcccc gccgtcgacg
     5941 gtcaccgagg agctgctcgc cgagttcgtg cggcccgtcc tggtgggcgc cgatcccatg
     6001 cggatcgagc acctctggcg cgccctctac aagtggcagc gcggcagcgg cggccggctc
     6061 accgaccggg tggtctgcgc cgtcgagctg gcgctttggg acctcgtcgg ccgcaaggtc
     6121 ggccagccgg tctggaagct cctcggcggc tatcgcgaca agatcctggc ctatggcagc
     6181 acgatgtgcg gcgacgatct ggcgaacggg ctcgcgacgc ccgaggacta cggccgctac
     6241 gccgagtggc tggtgcagaa gcgcggctac aaggcggtca agctgcacac ctggatgccg
     6301 ccgaggagct tccagaccgg ctggccgacc tt
//
LOCUS       DAOTFI010000262         6294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14697_length_6294_cov_5.944062, whole genome shotgun sequence.
ACCESSION   DAOTFI010000262 DAOTFI010000000
VERSION     DAOTFI010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14697_length_6294_cov_5.944062"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="P5558_20430"
     CDS             complement(<1..456)
                     /locus_tag="P5558_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26748.1"
                     /translation="MMGSEWQWRFWCLIPIGLCLGGNGPALALTALDSRPSDEVPLEL
                     TQRLQKLAGEIGWGSLFRTEQMVRADTAYGAGPVLAVTATPSMPVLAYLESRAWAAER
                     FLAEGDADRRFAELYVDPSTALVFDLLVIAETPEALRRDALIVTYLDGTG"
     gene            598..1350
                     /locus_tag="P5558_20435"
     CDS             598..1350
                     /locus_tag="P5558_20435"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRY26749.1"
                     /translation="MPTEVLFVGESWTTIGFHLKGFGIYTSGEYDEGGTPLIEALEAH
                     GCRVTYLRNHEVDKRFPRSRSELARYDVVVLSDVGADTFLLRPETLKQSKILGNPLQD
                     IADHVAEGAGFLMVGGYLSFSGFGGNACYPNTVIADILPVEMLRGDDRIERPEGLSPV
                     RLVDHDALAGVPEPWPPFLGYQRLVARPGSEVLLGAGDDPFLTLGRHGKGRVAAFASD
                     CSPHWGTPEFVGWAHYGRFWGQLTAWLAAGRG"
     gene            1406..2470
                     /gene="dctP"
                     /locus_tag="P5558_20440"
     CDS             1406..2470
                     /gene="dctP"
                     /locus_tag="P5558_20440"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY26750.1"
                     /translation="MTSLRRLLTATAALALAAIASPTVEAQEFTLRITNPDAPYVQID
                     DMKYPYYVFGMMSAFGETVEALSKGRIKAEIYHSGTLGDLRENMESVRMGVLEATTPS
                     EGPVSIFYPEIQVITIPYVFQNAAVAWEVMDGPFGQKLFDGMIEQGFRAVAIGENGGF
                     RIWGNNARKIETPDDMAGLKIRTMEIPAHQEMVKALGASPTAVPWMEVYSALQTGVVD
                     GAELPTVGSLQQNLHEVLEFITVDSHVYSLSFIIVSEKWFQSLPPDLQEAVLLAGRIA
                     TVTSRGLVQVTVNDALRYFSENGIEVTYLSGEAQAAFREKAQPGVIKWMEETLSPEIV
                     AEFMDSVRAAEAKLGMTPSL"
     gene            2547..3101
                     /locus_tag="P5558_20445"
     CDS             2547..3101
                     /locus_tag="P5558_20445"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY26751.1"
                     /translation="MLKRFCDRLAWITAVLSVVCLLVMTVSILVNVYFRYVLNAPLAW
                     PPELARFMMVAVTLLASSLAMRSGAHVGVSILVLRLPIRAQAWLFSLNSALILGFLLI
                     LLWYGWQLAFLEGPRQMAPSLGVSMMFAFIPLPLGAVLMIVHLAEITVEAWRRARIGE
                     SPFEAVYHDEAEAVALAAERTQEG"
     gene            3114..4400
                     /locus_tag="P5558_20450"
     CDS             3114..4400
                     /locus_tag="P5558_20450"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY26752.1"
                     /translation="MVVAFIAFFVFLLMGIPITFVLGLTAVLAFILKQDLVFLTLVPQ
                     RMFAGLNLFTIMAIPFFLLAAELMTLARITDDLVRFADALVGRLKGGLAHVNVVASAF
                     FAGISGSAISDVAGLGSIEIKAMTRAGYPKPFAAALTVASSLIGPIIPPSIIMVLYGS
                     IMQVSIAAMFLGGILPGLLMVAMLMAMIMWMADRQGFPRRLEKLPPGEIWQATKSAIL
                     ALLMPAIILGGILSGAFTATEAAAVAVAYALLIGLVVKRTLPLRDVPPMLVRVSILTS
                     VVFMVLATANILAWIIGLERIPDAIASGLTSITENPYLLFLLVNLVLIVVGMFMDIGA
                     AVIVLAPILGPTMIDAGIHPVHFGIVMSINLLVGLTTPPVGPCLFVAAGITGLRMEQL
                     SVAMLPFYGLQVVFLLAITYFPFLTLFLPRYFGYIA"
     gene            4397..5173
                     /locus_tag="P5558_20455"
     CDS             4397..5173
                     /locus_tag="P5558_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRY26753.1"
                     /translation="MTVETPAQIKSLGKVLQVLECFSRSERGLGLAEIAGRTRLPKAT
                     THRLLASLKSIGFIEQDHGGDQYRLGMKLFELGSVVLANMELPREAQPFVDRLAAMSG
                     AAVHLCVFDGAQAVFIDRRRADNATRNTLTLLEGAPVHCTAVGKAMLAFQPARVVERV
                     IAAGLTGFTATTLTTAERLREELALIRERGWAIDDEEHEPGTRCIAAPIRNGAGQVIA
                     AISASSTPEIISPDKDEAHARLVMGIAGELSRRLGHVPAG"
     gene            5238..>6294
                     /locus_tag="P5558_20460"
     CDS             5238..>6294
                     /locus_tag="P5558_20460"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY26754.1"
                     /translation="MRDFQLPGRSAAYASTAMAATSHPLATLTALDMLKAGGLAADAA
                     VAACAVQCVVEPQSTGIGGDCWALYSPKEGGVTSLNSSGWTPSAISAEGLRGEGLEAM
                     PDGGVASVTVPGAVRGWQALLDRHGSMGLDELLRPAIGYARNGFPVTPRVAFDWARAT
                     WRLERSAAGRDFYLPGGAAPVAGDTMRLEQLGATLETIARRGADAFYEGPLAAAMTAS
                     LKSFGGLHGEADFAEFRPEWTEPISATYRGHTVYQCPPNGQGVIALLLLQILEQLELE
                     GLDPNGAERLHLEAEATRLAFRDRDAVLADPRQADVPTDRLLDKAYAAALAARIDASA
                     AMPALPPPLLPEHKDTIY"
BASE COUNT          923 a         2197 c         2084 g         1090 t
ORIGIN      
        1 gcccgtgccg tccaggtagg tgacgatcag ggcgtcgcgg cgcagggctt cgggcgtttc
       61 ggcgatcacg aggaggtcga agacgagggc ggtggacggg tcgacgtaga gctcggcgaa
      121 gcgccggtcg gcatcgccct ccgccaggaa gcgctcggcc gcccaggccc ggctctcgag
      181 ataggccaag accggcatgg acggcgtggc cgtgaccgcc agaaccggcc cggcaccata
      241 ggcggtgtcc gcccgcacca tctgctcggt gcggaagagg gagccccagc cgatctcacc
      301 ggcgagcttc tgcagccgct gcgtgagctc gagtggcacc tcgtcggacg gccggctgtc
      361 gagcgcggtc agggccagcg ccgggccgtt gccaccgagg caaagcccga tcggtatgag
      421 acaccagaaa cgccattgcc actcactgcc catcatccgc ggcctccttg ccgcaacttc
      481 ctgcgatagc tccaattgac gacttgacgc ccgctcgcct gtttcctcat tatggaatca
      541 taatctggaa agcggaacaa ggagccgatc ggttccttcg gggaggaacg tcagatcatg
      601 ccaacagagg tgctgttcgt cggcgaatcc tggaccacga tcggctttca tctcaagggc
      661 ttcggcatct acaccagcgg cgagtacgac gagggtggca cgccgctgat cgaggcgctc
      721 gaggcgcatg gctgccgggt gacctatctg cgcaaccacg aggtcgacaa gcggtttccc
      781 cgcagccgca gcgagctcgc ccgttacgat gtcgtggtgc tgagcgatgt cggggcggac
      841 accttcctgc tcaggccgga gacgctcaag cagtcgaaga tcctgggcaa cccgctgcag
      901 gacatcgccg accacgtggc cgagggtgcc ggattcctga tggtcggcgg ctatctctcg
      961 ttctccggct tcggcggcaa tgcctgctat ccgaacacgg tgatcgccga catcctgccg
     1021 gtggagatgc tgcgtggcga cgaccggatc gagcggcccg aaggactgtc gccggtgcgc
     1081 ctcgtcgacc acgatgcgct ggccggtgtg ccagagccat ggccgccctt cctcggctat
     1141 cagcgactcg tcgcccggcc gggttccgag gtgctgctcg gtgccggcga cgatccgttc
     1201 ctcacgctcg gccgccacgg caaggggcgg gtcgccgcct tcgcctcgga ctgctcgcca
     1261 cactggggca cgccggagtt cgtcggctgg gcgcattacg gccgtttctg gggtcagctc
     1321 acagcctggc tcgctgccgg acgaggctga acgccgccca ccgtcatcca cgttcacagc
     1381 aagaaacgaa agggagacca gacccatgac cagccttcgc cgcctgctca ccgccacggc
     1441 cgccctggcc cttgccgcga tcgcgtcgcc gaccgtcgag gcgcaggagt tcacgctgcg
     1501 catcaccaat ccggacgcgc cctacgtcca gatcgacgac atgaagtacc cgtattacgt
     1561 gttcgggatg atgagcgcct tcggcgagac cgtcgaggcg ctgtcgaagg gtcggatcaa
     1621 ggccgagatc taccacagcg gcacgctcgg cgacctgcgc gagaacatgg aaagcgtgcg
     1681 catgggcgtg ctggaggcga ccacgccctc ggaaggcccg gtctcgatct tctatcccga
     1741 gatccaggtc atcacgatcc cctacgtctt tcagaacgcc gctgtcgcgt gggaggtgat
     1801 ggacgggccg ttcggccaga agctcttcga cggcatgatc gagcagggct ttcgcgccgt
     1861 ggcgatcggt gagaatggcg ggttccgcat ctggggcaac aatgcccgca agatcgagac
     1921 gccggatgac atggccgggc tgaagatccg gaccatggag atcccggcgc atcaggagat
     1981 ggtcaaggcg ctcggcgcct cgcccaccgc ggtgccctgg atggaggtct actcggccct
     2041 gcagaccggc gtcgtcgacg gcgccgagct gccgaccgtg ggctcgctgc agcagaacct
     2101 gcacgaggtg ctcgagttca tcacggtgga cagccacgtc tacagcctgt ccttcatcat
     2161 cgtcagcgag aagtggttcc agagcctgcc gccggacctg caggaggccg tgctgctggc
     2221 cggccggatc gccacggtga ccagccgcgg cctcgtccag gtgaccgtca atgacgcgct
     2281 tcgctacttc tccgagaacg gcatcgaggt gacctacctc tcgggcgagg cgcaggcggc
     2341 gttccgcgag aaggcgcagc ccggcgtcat caagtggatg gaggagacgc tctcgcccga
     2401 gatcgtcgcc gagttcatgg actcggtgcg cgccgccgag gcgaagctgg gcatgacccc
     2461 cagcctctga gaccacgggc ctccaggctc acgacccggg agttgtggct cccgggtcgt
     2521 ccctttcccc gcaccgggtg gcgtcgatgc tcaagcggtt ctgtgaccgg ctggcctgga
     2581 tcaccgcggt cctgagcgtc gtctgcctgc tggtgatgac ggtctcgatc ctcgtcaacg
     2641 tctacttccg ctacgtgctc aatgcacccc tcgcctggcc gcccgagctg gcacgcttca
     2701 tgatggtggc ggtgaccctg ctggccagca gcctcgccat gcgcagtggt gctcatgtcg
     2761 gcgtctcgat tctggtgctg cgcctgccga tccgcgccca ggcctggctc ttttcgctca
     2821 acagcgccct gatcctgggt ttcctgctga tccttctctg gtatggctgg cagctcgcct
     2881 tcctcgaggg gccgcggcag atggcgccct cgctcggcgt ctcgatgatg ttcgccttca
     2941 tcccgctgcc gctcggcgcc gtcttgatga tcgtccatct ggccgagatc acggtcgagg
     3001 cctggcggcg cgcccggatc ggtgagtcgc cgttcgaggc cgtctatcac gacgaggcgg
     3061 aggcggtggc cctcgcggca gagcggacgc aggagggctg agccgtggac ccgatggtcg
     3121 tcgccttcat cgccttcttc gtcttcctcc tgatgggcat ccccatcacc ttcgtcctcg
     3181 gcctgacggc cgtcctcgct ttcatcctga agcaggacct cgtcttcctg acgctcgtgc
     3241 cacagcgcat gttcgccggg ctcaacctct tcaccatcat ggcgatcccg ttcttcctgc
     3301 tcgccgccga gctgatgacg ctggcgcgga tcaccgacga cctcgtgcgc ttcgccgacg
     3361 cccttgtcgg ccggctcaag ggcggcctcg cccatgtcaa cgtggtggcg agcgccttct
     3421 tcgccggcat ctcgggctcc gccatctcgg atgtggccgg gctcggctcg atcgagatca
     3481 aggcgatgac cagggcgggc taccccaagc ccttcgcggc ggcgctcacc gtcgcctcgt
     3541 cgctgatcgg gccgatcatt ccgcccagca tcatcatggt gctctacggc tcgatcatgc
     3601 aggtctcgat cgccgccatg ttcctcggcg gcatcctgcc cgggctgctc atggtggcga
     3661 tgctgatggc catgatcatg tggatggcgg accggcaggg cttccccaga cggctcgaga
     3721 agctgccgcc gggggagatc tggcaggcga cgaagagcgc catcctcgct ttgctgatgc
     3781 cggcgatcat cctgggcggc atcctctccg gcgccttcac cgccaccgag gcggcggcgg
     3841 tcgccgtggc ctatgcgctg ctcatcgggc tcgtggtcaa gcgcaccctg ccgctccgcg
     3901 acgtgccgcc gatgctggtc cgcgtctcga tcctcacctc ggtcgtcttc atggtgctgg
     3961 ccacggccaa catcctcgcc tggatcatcg gcctcgagcg cattccggac gccatcgcga
     4021 gcgggctcac cagcatcacc gagaacccct atctcctgtt cctgctggtc aatctcgtgc
     4081 tgatcgtcgt cggcatgttc atggatatcg gcgctgcggt catcgtgctc gcaccgatcc
     4141 tcgggcccac catgatcgat gccggcatcc atcccgtgca tttcggcatc gtcatgtcga
     4201 tcaacctgct ggtggggctc accacgccgc cggtcggccc ctgcctcttc gtcgccgccg
     4261 gcatcaccgg gctcaggatg gagcagctct cggtggcgat gctgccgttc tacgggctgc
     4321 aggtggtgtt tctcctggcg atcacgtatt tccctttcct gaccctcttc ctgccccgct
     4381 atttcgggta catcgcgtga ccgtcgagac cccggcgcag atcaagtcgc tcggcaaggt
     4441 gctgcaggtc ctcgagtgct tctcgcgatc cgagcgcggc ctcggcctcg ccgagatcgc
     4501 cggccgcacg cgcctgccca aggccacgac ccaccgcctg ctcgcctcgc tcaagtcgat
     4561 cggcttcatc gagcaggatc acggcggcga ccagtatcgg ctcggcatga agcttttcga
     4621 gctgggctcc gtcgtgctcg ccaacatgga gctgccgcgc gaggcgcagc ccttcgtcga
     4681 ccgcctcgcg gcgatgtccg gggccgccgt ccatctctgc gtcttcgacg gcgcccaggc
     4741 cgtcttcatc gaccggcgcc gcgccgacaa cgcgacccgc aacacgctca ccttgctcga
     4801 gggggctccc gtgcactgca ccgcggtcgg caaggccatg ctggcgttcc agccggcgcg
     4861 cgtcgtcgag cgggtgatcg cggccgggct cacgggcttc acggcaacca cgctgaccac
     4921 ggccgagcgg ctgcgtgagg agctggccct gatccgcgag cgcggttggg ccatcgacga
     4981 cgaggagcac gagcccggca cgcgctgcat cgccgccccc atccgcaacg gtgccggcca
     5041 ggtgatcgcg gcgatctcgg cgtccagcac gcccgaaatc atctcgcccg acaaggacga
     5101 ggcgcatgcg cggctggtca tgggcatcgc cggcgagctg tcgcggcggc tcggccatgt
     5161 gccggctggc tgactgggta ttcgaggtga cccgggccgg cacttgctgc tagacgggcg
     5221 agaaggagac agaagccatg cgcgattttc agcttcccgg gcgttccgct gcctatgcct
     5281 cgacggcgat ggcggcgacc tcgcacccgc tggcgacgct caccgccctc gacatgctga
     5341 aggccggcgg ccttgctgcc gacgctgccg tcgcggcctg tgccgtgcag tgcgtcgtcg
     5401 agccgcaatc gaccggcatc ggcggcgatt gctgggcgct ctattcgccc aaagagggtg
     5461 gcgtgacgtc gctcaactcg tcgggctgga cgccttcggc gatctcggcc gaagggctgc
     5521 gcggcgaggg gcttgaagcc atgccggatg gcggcgtggc gagcgtcacc gtgccgggtg
     5581 cggtgcgcgg ctggcaggcg ctgctggacc ggcacggctc gatggggctg gacgagctgc
     5641 tgcggccggc catcggctat gccagaaacg gctttcccgt gacaccccgg gtcgccttcg
     5701 actgggccag ggcaacctgg cggctggagc ggagtgcggc cggtcgcgac ttctatctgc
     5761 cgggcggggc ggcgccggtg gccggcgaca cgatgcggct ggagcagctc ggcgccacgc
     5821 tcgagacgat cgcccggcgc ggtgccgatg ccttctacga aggaccgctg gcggcggcca
     5881 tgacggccag cctgaagagc ttcggcgggc tgcatggcga ggccgatttc gccgagttcc
     5941 ggccggaatg gaccgagccc atcagcgcca cctaccgggg ccatacggtc taccagtgcc
     6001 cgcccaacgg ccagggcgtg atcgcgctcc tgctcctgca gatcctggag cagctcgagc
     6061 tcgaagggct cgacccgaac ggggcggagc ggctgcatct cgaggccgag gcgacccgcc
     6121 tcgcctttcg cgaccgggat gccgtgctcg ccgacccgcg ccaggcggat gtcccgaccg
     6181 accggctgct cgacaaggcc tatgccgccg cgctcgccgc ccggatcgac gccagcgccg
     6241 ccatgccggc gttgccgccg ccgctgctgc cggagcacaa ggacacgatc tacc
//
LOCUS       DAOTFI010000263         6274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14751_length_6274_cov_6.939540, whole genome shotgun sequence.
ACCESSION   DAOTFI010000263 DAOTFI010000000
VERSION     DAOTFI010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14751_length_6274_cov_6.939540"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="P5558_20465"
     CDS             complement(<1..208)
                     /locus_tag="P5558_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26755.1"
                     /translation="MLASLKRLPGASWLIHPVTLVYLVVIAIWITAGIMKPGFARIGH
                     LRYLLELAAVIGIAAAGQTLVIIAA"
     gene            complement(213..1154)
                     /locus_tag="P5558_20470"
     CDS             complement(213..1154)
                     /locus_tag="P5558_20470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26756.1"
                     /translation="MTGAALQLRLLWQRNAGLYGLYLLLIALLAVYAWLHPGLLSVAG
                     VARFTQNWFPIAVVALAQTLIMLMGGIDLAIGATVSLGCVLAASWIGDSYGGAALGIA
                     LAVGVGAVIGGTVGAIVALLRIPAIVVTLAASFIIGGIALLVMPRPGGHVPAWLSDGL
                     AGETPTALVLLLCLLVAWRILQATPLGQDINAAGNNPHGAFRSGVNVARARIVAYAIS
                     GALSAFAGVYVAAQTGSGDPIIGGPFILDSITAAVLGGVGFLGGIGAFRGAIAGSLLL
                     AVMINVMFFLGFPPVAQYIAKGLIIVVAMGLRLLRER"
     gene            complement(1151..2653)
                     /locus_tag="P5558_20475"
     CDS             complement(1151..2653)
                     /locus_tag="P5558_20475"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY26757.1"
                     /translation="MSAPLLAARGLAKRFFENTVLDGVSIEIRPGRVHALLGENGAGK
                     STLINLLSGVLRPDAGSIAIDGTEHAALTPALARELGIAVVQQELSLAPNLSVAENIV
                     MGAVPHRGPFTDYTRLAAETGRLCQRLGLDMPLDTPVDRISLGRRQLIEIAKALYRRP
                     RLLILDEPTSSLTGHEVRILFGLLRELQADGVAILYISHRLNEVLELCDWVTVLRDGQ
                     RTADQALDGSDPASLVRLMVGREPGDLFPPRPAHNPGETAIAVRGLVAEGISCVDLEL
                     RHGEILGIGGLLGQGQEETLRALFGDLQAKAGEIRLAGQVQRHLTPIRAIEAGIVYVP
                     ADRKREAVLLPLSIRFNLTLPSLRRLARRGLRRLQDEARTATDAIRDFAIRVADPAQP
                     TLHLSGGNQQKVAIARWLPLGPRILLLDDPTRGVDVETKREIYLRLRQMAADGVAIVL
                     SSSDTLELIHVADRVAVFRGGRVVAHLDRDQLSEEAIVAASLGVEAELAA"
     gene            complement(2681..3739)
                     /locus_tag="P5558_20480"
     CDS             complement(2681..3739)
                     /locus_tag="P5558_20480"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26758.1"
                     /translation="MSSSLKLALSCAACAIALTAMSTATSTPALALAKCMTGEREAPY
                     TIGWANIYSIPTWMKQTQGTIEDVFSQLQEQGLTDELVVTDAEGNANTQITQIQSMID
                     ADVDGIVVIAGSSTALDRVIAQACEKGIAVINVDSLVETDEITAKINTDSNMWGQLAA
                     EWMVEQLGGKGKIIIMNGPAGVSVSEDRRAGATPVLEANPGIEVLTETNTPYNAAPAQ
                     EAMTSLLFQYPEIDGILSFGGALTAGAILAMDRQGRDVPPSTGENYRQFLELWSEKGF
                     PGWATMQPNWLGGLGVYAAVKALEGEDIAAFVEVPLPIITDENLPEYLARAEDFPADG
                     YIYSPYDLALFDEILGLK"
     gene            complement(3791..4714)
                     /locus_tag="P5558_20485"
     CDS             complement(3791..4714)
                     /locus_tag="P5558_20485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY26759.1"
                     /translation="MRYTGPILDAHHHLWDLKLEKHPWLVPQPGRRKVFGDPTPLYRD
                     YLPDDLERDAAGLHLVGSVHIEASWDRSDPVGETRWIAGLKQRHRLPTVAVVRAPLDD
                     PGVGAILEAQRAFDFVRGVRFILSWHEDPEKRFVERADYMTDSRWLAGFALLREFGLS
                     FDMLIYPGQLGDAADLADRFPDTTIIVNHAGSPVDRDAVAMDHWRRGLKELAKRPNVR
                     LKISDLCAYDHDWTLESLRPVVLDCLDAFGLGRAMFASDFPVAGLHAGYAQMYDAFAD
                     IVQDLTAEEQRALFHDNAVRDYRIDKTDSRP"
     gene            complement(4717..5322)
                     /locus_tag="P5558_20490"
     CDS             complement(4717..5322)
                     /locus_tag="P5558_20490"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRY26760.1"
                     /translation="MQDGKKSDERPRRPAAEAVRGIDAGLGDLWWWFLARGVLAGLLG
                     LALLVWPSWSVEVLAVVLGFFLLIDGVAGLIGTMRARQTAMSLTPALISIVVGGILLL
                     WPGATLRMLLIVVGIWAIASGVGYLMSGRGTAADVQADGHLKVIGITVTLVGVILFFW
                     PSTGIVTVAWVLAAAAFAIALVLLFVAMRLKRLKDRIGARA"
     gene            complement(5338..6159)
                     /locus_tag="P5558_20495"
     CDS             complement(5338..6159)
                     /locus_tag="P5558_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY26761.1"
                     /translation="MVDAGTRLDHLLVTAGAWLLAVLWILPLLFALWAAFHPPLYAAR
                     FDLTAPLTLDNFTRAMAAAPFARYMLNTLMLVTMTLAGQLVISTLAAFAFARLEFWGR
                     DLLFFLVLVQLMITPDVLLVENYRIVSSLGILDTILAIGLPYMASAFGIFLLRQTFKT
                     VPKELDDAARIEGCTTLGILLRVYVPLARPIYVAFGLVSVSFHWNNFLWPLIVTSSVE
                     TRPVTVGLQVFASVDQGIDWSVISAATLFTSAPLLIAFLLFQRQFVQSFMRAGIK"
BASE COUNT         1032 a         1901 c         2457 g          884 t
ORIGIN      
        1 cggcggcgat gatgaccagc gtctggccgg cggcggcgat gccgatcacg gcggcgagct
       61 cgagcaggta gcggagatgg ccgatccggg cgaagcccgg cttcatgatg ccggccgtga
      121 tccagatggc gatgacgacg agatagacga gggtcaccgg atggatgagc cagctcgcgc
      181 cgggcaggcg tttcaggctc gcgagcatcg gctcagcgct ccctgagcag gcggaggccc
      241 atcgcgacca cgatgatcag gcccttggcg atgtactggg cgaccggcgg gaatcccagg
      301 aagaacatga cgttgatcat gacggcgagg agcaggctgc cggcgatggc gccgcgaaac
      361 gcgccgatgc cgccgaggaa gccgacgccg ccgagcacgg ccgcggtgat cgaatcgagg
      421 atgaaggggc cgccgatgat cggatcgccc gagcccgtct gggccgcgac atagaccccg
      481 gcgaaggcgg agagggcgcc ggagatggcg taggcgacga tgcgtgccct tgcgacgttc
      541 acgccgctgc ggaaggcgcc atgtgggttg ttgccggcgg cgttgatgtc ctggcccagg
      601 ggggtggcct gcaggatgcg ccaggcgacg agcaggcaga ggaggagcac gagggcggtc
      661 ggcgtctcgc cggcgaggcc gtcggagagc caggccggga cgtggccgcc agggcgcggc
      721 atgacgagca gcgcaatgcc gccgatgatg aaggaggcgg cgagcgtcac gacgatggcc
      781 gggatgcgca ggagcgccac gatggcgccg accgtgccgc cgatcacggc accgacgccg
      841 acggcgagcg cgatgccgag ggcggccccg ccatagctgt cgccgatcca gctcgcggcc
      901 agcacgcagc cgaggctgac cgtcgcgccg atggcgaggt cgatgccgcc catcagcatg
      961 atgagggtct gggcgagggc gacgacggcg atggggaacc agttctgggt gaagcgggcg
     1021 acgccggcga cgctgagcag gcccggatgc agccaggcgt agacggcgag cagggcgatg
     1081 agcagcagat agagcccgta gagcccggca ttgcgctgcc agaggaggcg gagctgcagc
     1141 gccgcgccgg tcacgccgcc agctccgcct cgacgccgag cgacgccgcg acgatcgctt
     1201 cctcggagag ctggtcacgg tcgagatgtg cgaccacccg gccgccgcgg aagacggcga
     1261 cgcggtcggc cacatggatc agctcgagcg tgtcggagct cgagagcacg atggcgaccc
     1321 cgtcggccgc catctggcgc aggcgaaggt agatctcgcg cttggtctcg acatcgacgc
     1381 cgcgggtggg atcgtcgagg aggaggatgc ggggcccgag cggcagccag cgggcgatgg
     1441 cgaccttctg ctggttgccg cccgagagat ggagcgtggg ctgggccgga tcggcgacgc
     1501 ggatggcgaa gtcgcgaatg gcgtcggtgg cggtccgggc ctcgtcctgc aggcggcgca
     1561 ggccgcgccg ggcgagccgg cggagcgacg gcagggtgag gttgaagcgg atcgacaggg
     1621 gcaggagcac ggcctcgcgc ttcctgtcgg ccggcacgta gacgatgccg gcctcgatcg
     1681 cccggatcgg cgtgaggtgg cgctgcacct ggccggcgag gcggatctcc ccggctttcg
     1741 cctgcaggtc gccgaagagg gctctcaacg tctcttcctg gccctggccg agcaggccgc
     1801 caatgccgag gatctcgcca tggcggagct cgagatcgac acaagagatg ccctcggcga
     1861 cgaggccccg gacggcgatg gcggtctcgc cggggttgtg cgccgggcgt ggcgggaaga
     1921 ggtcgccggg ctcgcggccg accatcagcc ggacgaggct ggccggatcg ctaccgtcga
     1981 gagcctggtc cgccgtgcgc tggccgtcgc gcaggacggt cacccagtcg cagagctcca
     2041 gcacctcgtt cagccggtgg gagatgtaga ggatggcaac cccatcggcc tgcagctcgc
     2101 gcagcaggcc gaagaggatg cgcacctcgt ggccggtgag cgaggaggtg ggctcgtcga
     2161 ggatcaggag gcgagggcgg cgatagagag ccttggcgat ttcgatgagc tggcgccggc
     2221 cgagcgagat gcggtcgacc ggggtatcga ggggcatgtc gaggccgagc cgctggcaga
     2281 ggcggccggt ctcggcggcg agcctcgtgt agtcggtgaa ggggccgcgg tgcggcaccg
     2341 cacccatgac gatgttctcg gcgaccgaga gattgggcgc gaggctcagc tcctgctgga
     2401 cgacggcgat gccgagctcg cgggcgaggg cgggggtgag ggcggcgtgc tcggtgccgt
     2461 cgatggcgat gctgccggca tcggggcgaa gcacgcccga gagcaggttg atgagcgtcg
     2521 acttgcccgc gccgttctcg ccgagcagcg catggacgcg gccggggcgg atctcgatgg
     2581 agacaccgtc gaggacggtg ttctcgaaga agcgcttggc gaggccccgg gccgcgagca
     2641 gcggtgccga catcgtttgc cgctgctcgc cgccgcttgc ctacttcagg ccgaggatct
     2701 cgtcgaagag ggcgagatcg tagggcgagt agatgtagcc gtcggccggg aagtcctcgg
     2761 cgcgggccag gtactcgggg aggttttcgt cggtgatgat cggcagcggc acctcgacaa
     2821 aggcggcgat gtcctcgcct tcgagcgcct tgacggcggc gtagacgccg aggccgccca
     2881 gccagttggg ctgcatggtg gcccagccgg ggaagccctt ctcgctccag agctcgagga
     2941 actggcggta gttctcgccg gtgctcggcg gcacgtcgcg accctggcgg tccatggcga
     3001 ggatggcgcc cgcggtgagg gcgccgccga agctgaggat gccgtcgatc tccgggtact
     3061 ggaagagcag gctggtcatc gcctcctggg ccggcgcggc gttgtagggg gtgttggtct
     3121 cggtcaggac ctcgatgccg ggattggcct cgagcaccgg cgtggcgccg gcgcgccgat
     3181 cctcgctgac cgagacgccg gccgggccgt tcatgatgat gatcttgccc ttgccgccga
     3241 gctgctcgac catccattcg gccgcgagct ggccccacat gttggaatcg gtgttgatct
     3301 tggcggtgat ctcgtcggtc tcgaccaggc tgtcgacatt gatgacggcg atgcccttct
     3361 cacacgcctg ggcgatgacc cggtcgaggg cggtcgacga gccggcaatg acgacgatgc
     3421 cgtccacatc ggcgtcgatc atcgactgga tctgggtgat ctgggtgttg gcgttgccct
     3481 cggcgtcggt gacgacgagc tcgtccgtca ggccctgctc ctggagctgg ctgaagacgt
     3541 cctcgatggt gccctgagtc tgcttcatcc aggtcgggat cgagtagatg ttggcccagc
     3601 cgatggtgta gggcgcctcg cgctcgccgg tcatgcactt ggcgagcgcc aaagcaggcg
     3661 tgctggtggc cgtgctcatg gcggtcaggg cgatggcgca ggcggcgcag gacagcgcca
     3721 gcttcaggct gctcgacatg gatgcttccc ccggtttcgc cggcctttcc ggcagttgta
     3781 tgacgatatg ctaaggtcgc gaatccgtct tgtcgatccg gtagtcgcgg accgcgttgt
     3841 cgtggaagag tgcccgctgc tcctccgctg tcaggtcctg cacgatgtcg gcgaaggcgt
     3901 catacatctg ggcgtagccg gcatgcagcc cggccaccgg gaagtcggag gcgaacatcg
     3961 cccggcccag accgaaggcg tcgaggcagt cgagcacgac gggcctgagg ctctcgagcg
     4021 tccagtcgtg gtcgtaggcg cagagatcgg agatcttgag gcggacattg gggcgtttcg
     4081 ccagctcctt cagcccgcgc cgccagtgat ccatggcgac ggcgtcgcga tcgaccgggc
     4141 tgccggcgtg gttgacgatg atggtggtgt ccggaaatcg gtcggcgaga tcggccgcgt
     4201 cgccgagctg gcccggatag atgagcatgt cgaaagagag gccgaactcc cggagcaggg
     4261 cgaagccggc cagccacctg gagtcggtca tgtagtcggc gcgttcgacg aagcgcttct
     4321 ccgggtcctc gtgccagctc aggatgaagc ggacgccgcg gacgaagtcg aaggcgcgct
     4381 gggcttcgag aatggcgccg acgccggggt cgtcgagcgg ggcgcggacg acggcgaccg
     4441 tcggcaggcg gtgtcgctgc ttcaggccgg cgatccagcg cgtctcgccg acggggtcgc
     4501 tcctgtccca gctcgcctcg atatgcaccg agcccacgag gtggagcccg gcggcgtcgc
     4561 gctcgaggtc gtccggcagg tagtcgcggt agagaggggt cgggtcgccg aagaccttgc
     4621 ggcggccggg ctggggcacc agccaggggt gcttctcgag cttcaggtcc cagaggtggt
     4681 ggtgggcgtc gaggatcggt cccgtgtagc gcatggtcag gctcgtgctc cgatccggtc
     4741 cttcagccgc ttcagccgca tggcgacgaa gagcaggacc agggcgatgg cgaaggctgc
     4801 ggcggcgagc acccaggcaa ccgtgacgat gccggtcgag ggccagaaga agaggatgac
     4861 gccgaccagg gtcacggtga tgccgatgac cttgagatgg ccgtcggcct ggacgtcggc
     4921 agcggtgccg cgccccgaca tgaggtagcc gacgcccgag gcgatggccc agatgccgac
     4981 gacgatcagg agcatgcgga gggtcgcccc gggccagagg agcaggatgc cgccgacgac
     5041 gatgctgatc agggcggggg tgaggctcat ggcggtctgc cgggcgcgca tcgtgccgat
     5101 caggccggcc acaccgtcga tcaggaggaa gaagcccagc acgaccgcca gcacctcgac
     5161 gctccagctc ggccagacga ggagggcgag gccgagcagg ccggcgagca cgccgcgtgc
     5221 caggaaccac caccagagat cgccgagccc ggcgtcgatg ccgcggacgg cctcggccgc
     5281 ggggcgccgc ggtcgctcgt cgctcttctt cccgtcctgc atgacgcgcc ctcctgccta
     5341 tttgatgccg gcccgcatga agctttgcac gaactggcgc tggaagagaa ggaaggcgat
     5401 gagcagcggg gccgaggtga agagggtggc ggccgagatc accgaccagt cgatgccctg
     5461 gtcgaccgag gcgaagacct gcaggccgac ggtcaccggg cgcgtctcga ccgagctcgt
     5521 gacgatcaag ggccagagga agttgttcca gtggaagctg accgagacga ggccgaaggc
     5581 gacgtagatc ggccgggcga ggggaacgta gacgcgcaag aggatgccga gcgtggtgca
     5641 gccctcgatg cgggcggcgt cgtcgagctc cttcggcacg gtcttgaagg tctggcgcag
     5701 gaggaagatg ccgaaggccg aggccatgta gggcaggccg atggcgagga tggtgtcgag
     5761 gatgccgagg ctgctgacga tgcggtagtt ctccacgagc agcacgtccg gcgtgatcat
     5821 gagctggacg aggaccagga agaagaggag gtcgcggccc cagaactcga gcctggcgaa
     5881 ggcgaaggcg gcgagcgtgc tgatcacgag ctggccggcc agggtcatgg tgaccagcat
     5941 cagcgtgttc agcatgtagc gggcgaaagg ggcggccgcc atcgcccggg tgaagttgtc
     6001 gagcgtcagg ggggcggtga ggtcgaagcg ggcggcatag agcggcgggt ggaaggccgc
     6061 ccacagagcg aagaggagcg gcaggatcca caagacggcc aggagccagg cgccggcggt
     6121 gacgaggaga tggtcgagcc gcgtgcccgc gtcgacgatg cgggcggcgg cggacccttg
     6181 ggccagggcg gcgctcgtca ccggtagtgg atccgcttgt cgagcacggt cacctgcagc
     6241 accgagacgg tgatcagcac cgcgagcagg acga
//
LOCUS       DAOTFI010000264         6267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14780_length_6267_cov_6.447682, whole genome shotgun sequence.
ACCESSION   DAOTFI010000264 DAOTFI010000000
VERSION     DAOTFI010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14780_length_6267_cov_6.447682"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..441)
                     /locus_tag="P5558_20500"
     CDS             complement(58..441)
                     /locus_tag="P5558_20500"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRY26762.1"
                     /translation="MEDWKGWLLNRVTITFGLIAVVVIAWNLYVQAHDDGILEGQVVN
                     EAGEPVAGAKVVLSERTIVSMTSIAETVTDADGRFHFEGHDRHALVLTAEMPDLARSA
                     RMEVQLYFKNQNRRLPSPIVLSGAA"
     gene            complement(446..1258)
                     /locus_tag="P5558_20505"
     CDS             complement(446..1258)
                     /locus_tag="P5558_20505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY26763.1"
                     /translation="MHRYPRWPIHLILVIVSFVMLVPFYWVIKTSITGENIFNYPPSL
                     WPVDPQPFFYVDAWYAIPFPRFFLNSVIVAGMAIVANLIINAAAGFALTKSFPGKRWM
                     ILLLLACMMIPFHATIIPAYLITAQLGLLNTHLGLALPAFSHIICIFLFKASFDAIPP
                     SLIQAAKIDGVPEWQILLKIMLPLAKPAVATNIILSFIWSWNDFLWPLIIVRDVEMQT
                     LPLGLSTFLAYFEDTTGSLYAFCVMVLAPGLLVFFLAQKQFIEGLTSGARKG"
     gene            complement(1271..2170)
                     /locus_tag="P5558_20510"
     CDS             complement(1271..2170)
                     /locus_tag="P5558_20510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY26764.1"
                     /translation="MFARKSHREYLLVWLMLLPALAIFMLFRIIPLLWNGVLSFQFWS
                     PTKPAEFAGLYHYEEMFLYDDVFWEALVNTFKYMLTAPIAIILALLLALLVNSRIRGR
                     GIYRTIIFLSYPLMVVAVGIIWRWLFDQRVGLINFSLRSFGLIDEPIAFLESFATALP
                     SVIAAAIWQIVGFFMIILLTGLQSIPQNLYEAASIDGASRWSQFWRITLPLLRPSLFL
                     CFVIGIIASFTSFDLIYVMTGGGPGHATELLITYVYKTAFTLTQFDYAGALTIVMFLL
                     FVGIALIGNLLAGGDAGRVDIAD"
     gene            complement(2230..3480)
                     /locus_tag="P5558_20515"
     CDS             complement(2230..3480)
                     /locus_tag="P5558_20515"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY26765.1"
                     /translation="MLRRHLMATVAGALLLAGLSGQAALAQEKKLTLLTWNLPIYEEK
                     FRGWIADFEEMHPDFTVEWLDKKGTEFGAFYQTQLVAGTAPDIIDVQGMLWAEYAAND
                     GLVPLDPYLEANPEVRDRYNAEGMKLWQYDGKQYMLPYYFSKTLMVYNKKLFAEANID
                     APPSSFDELLDAAYKIKDTGEGRTGFITLNFDWLYWPLMAMNGIELLNEDMTEAAFNT
                     PEMVDVVTRLAKATTDGAINPISWTGRWVEPNNAFAAGDIGMYQGHGAALFWIMGQAD
                     WANADTLGVVEWPGGWGVPNAHGLGISASTEHPDEAFDFIQIALNDKWQTIMADNFTI
                     LTLNNSVDEALIAGLDDPLKAATLELSLANMDKQTGNWVTPLDARIKDAFWPDIQAAL
                     LGQRDPAEAIADAENKVNRVLRRG"
     gene            complement(3509..4549)
                     /locus_tag="P5558_20520"
     CDS             complement(3509..4549)
                     /locus_tag="P5558_20520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY26766.1"
                     /translation="MSGARTLRWGVIGYGRFGQLHAAAIARGDATELAAVATASEATA
                     ALARSHHPDAAVTTDWRKLVDDPGIDVVDIVAPNHLHAAMAKRALEAGKPVLVEKPLA
                     TTLADCDMLVETVDRTGGLLSVGFELRLSVQWRRIKELIDAGRIGRPTFVHVALFRHP
                     YRPGASGWRQSAGEVGSWILEEPVHFYDLAMWYLARSGEPVAVNAQGTAGREPGLIDN
                     LVSAVRFDDGALAVVSQTLAGFGHHLVVEVSGTEGAMRATWSAGHAASTEPCFDLHVG
                     PIGLEAPERVPLDEPSGEVFELEKQIGLTNAAFAEGRTLVDVREGRRAVVVCLEAERS
                     VREGREVPLRFA"
     gene            complement(4546..5403)
                     /locus_tag="P5558_20525"
     CDS             complement(4546..5403)
                     /locus_tag="P5558_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26767.1"
                     /translation="MDEATDAALAVPAEALGRDSPIRVERVADGAALVRRLADDLFAD
                     YRAARAAGREKVVVIVPVGPVGALDLVAEDCNRTGESLADLVVINMDEYLEPDGRRFI
                     AASDPLSFRGHMQRHFWDVLRPDLAPPEGQRIFPDPIDPANVGLAIERFGGVDVCYGG
                     VGITGHVAFNDPPAPGETIDTESFAALPSRVVELSTETLVINSVTACRGNIDRIPRLA
                     VTVGMREILASRLVRLYLNRPWQSAIVRKLLHGPVTAAVPASLLQRHADCRAVLTDEV
                     AQLPEPTLR"
     gene            complement(5396..>6267)
                     /locus_tag="P5558_20530"
     CDS             complement(5396..>6267)
                     /locus_tag="P5558_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26768.1"
                     /translation="FQDYALYPHMSVYENMAFGLRYRKYAKAEIKKRVANAARILDIE
                     PLLLRRPKELSGGQRQRVAMGRAIVRDPQVFLFDEPLSNLDAKLRVQMRTEIKRLHRR
                     VSTTVVYVTHDQVEAMTLADRIVIMRGGLIEQVGTPDEVYNHPRTSYVAGFIGSPTMN
                     LVPVEIDGDAREAVLRLPDGRLLPLPAEQLAMASGARGGPVLMGLRPEDFHAETLGGP
                     SLDIEPDVIEPLGSDTLVFFSIGGAELVARLPPRHVQGQPERLTLHVDPTKLHLFDAA
                     SGRALWLEGAGHG"
BASE COUNT         1080 a         1990 c         2195 g         1002 t
ORIGIN      
        1 cgtcggcatg cgggatgacg atgcgggcgt ccggcggcag gttctccgca ttgccgttca
       61 tgccgccccg ctcagcacga tgggcgaggg caggcgccgg ttctggtttt tgaaatagag
      121 ctgcacctcc atccgtgccg acctcgcgag atccggcatc tccgcggtca gcacgagcgc
      181 atgacgatcg tgcccctcga agtggaatct gccatccgca tcggtcaccg tctcggcgat
      241 cgacgtcatg ctgacgatgg tccgctcgct cagcaccacc ttcgcacccg ccacgggctc
      301 gcccgcctcg ttcacgacct gtccttcgag aatgccgtcg tcgtgcgcct gaacgtagag
      361 gttccaggcg atgaccacga cggcgatcag gccgaaggtg atggtgacgc ggttgaggag
      421 ccagcccttc cagtcttcca agggatcagc cctttctcgc gccggaggtg aggccctcga
      481 tgaactgctt ctgcgccagg aagaagacga ggagccccgg ggccaggacc atgacgcaga
      541 aggcatagag cgaccctgtc gtgtcctcga aataggcgag gaaggtcgag agccccaagg
      601 gcagcgtctg catctcgacg tcgcggacga tgatcagggg ccagaggaag tcgttccagg
      661 accagatgaa cgacaggatg atgttggtgg cgaccgcggg cttggccagg ggcagcatga
      721 tcttgagcag gatctgccat tccggcacgc cgtcgatctt ggccgcctgg atgagcgacg
      781 gcgggatcgc gtcgaagctc gccttgaaga ggaagatgca gatgatgtgc gagaaggccg
      841 gcagcgccag gccgagatgg gtgttcaaga ggccgagctg ggccgtgatc aggtaggccg
      901 ggatgatcgt cgcgtggaac gggatcatca tgcaggcgag caggaggagg atcatccagc
      961 gcttgcccgg gaagctcttg gtcagggcga agccggccgc cgcgttgatg atcaggttgg
     1021 cgacgatcgc catgccggcg acgatcacgc tgttgaggaa gaagcgtggg aaggggatcg
     1081 cgtaccaggc atcgacgtag aagaagggct gcggatcgac cggccagagc gacggcgggt
     1141 aattgaagat gttctcgccg gtgatgctcg tcttgatgac ccagtagaag ggcacgagca
     1201 tgacgaacga gacgatcacc aggatcaggt ggatcggcca gcggggatag cgatgcatcg
     1261 acttgctccc ctagtccgcg atgtcgacgc ggccggcatc gccgccggcg agcaggttgc
     1321 cgatgagcgc aatgccgacg aagagcagga acatgacgat ggtcaacgcc ccggcatagt
     1381 cgaactgggt cagcgtgaaa gccgtcttgt agacataagt gataagcagc tcggtggcat
     1441 ggccgggtcc gccgccggtc atcacgtaga tcaggtcgaa gctggtgaag gacgcgatga
     1501 tgccgatcac gaagcagagg aaaagcgagg gccggagcag cggcagggtg atccgccaga
     1561 actggctcca gcggctggcc ccgtcgatcg aggcggcctc gtagaggttc tgcgggatgc
     1621 tctgcagccc ggtgagcaga atgatcatga agaagccgac gatctgccag atggcggcgg
     1681 cgatcaccga ggggagcgcc gtggcgaagc tctcgaggaa ggcgatgggc tcgtcgatca
     1741 ggccgaacga gcgcaggctg aagttgatca ggccgacccg ctggtcgaag agccagcgcc
     1801 agatgatgcc gaccgccacg accatgagcg ggtagctgag gaagataatg gtgcggtaga
     1861 tgccgcggcc cctgatccgg ctgttgacga gcagcgccag cagcaaggcg aggatgatcg
     1921 cgatgggcgc cgtcagcatg tacttgaagg tgttgaccag cgcttcccag aagacgtcgt
     1981 cgtagaggaa catctcctcg tagtgataga ggccggcgaa ctccgcgggc ttggtcggcg
     2041 accagaactg gaacgagagc acgccgttcc agagcagcgg gatgatgcgg aacagcatga
     2101 agatggcgag cgccggcagc agcatcagcc acaccagcag gtactcgcga tggctcttcc
     2161 tggcgaacac gcagtcgttc ccgaagcgag cgagggaggc gccgccggcg gcgacgcctc
     2221 ccgctggtct cagccgcgcc ggagcacgcg gttgaccttg ttctcggcgt cggcgatcgc
     2281 ctcggccggg tcgcgctggc cgaggagagc tgcctggatg tcgggccaga aggcgtcctt
     2341 gatccgggcg tcgaggggcg tcacccagtt gccggtctgc ttgtccatgt tggcgagcga
     2401 gagctcgagc gtcgccgcct tgagcgggtc gtcgagcccg gcgatgagcg cctcgtcgac
     2461 cgagttgttc agcgtcagga tggtgaagtt gtccgccatg atcgtctgcc acttgtcgtt
     2521 cagggcgatc tggatgaagt cgaaggcctc gtccggatgc tcggtcgagg ccgagatgcc
     2581 gaggccgtgc gcgttcggga caccccagcc gcccggccac tccaccacgc cgagcgtgtc
     2641 ggcattggcc cagtcggcct ggcccatgat ccagaagagt gccgcgccgt ggccctggta
     2701 catgccgatg tcgccggcgg cgaaggcatt gttgggctcg acccagcggc cggtccagga
     2761 gatcgggttg atcgccccgt cggtcgtcgc cttcgcgagg cgggtcacga cgtcgaccat
     2821 ctcgggcgtg ttgaacgcgg cctccgtcat gtcctcgttg aggagctcga taccgttcat
     2881 cgccatgagc ggccagtaga gccagtcgaa gttcaaggtg atgaagccgg tgcgcccctc
     2941 gcccgtgtcc ttgatcttgt aggcggcgtc gagcagctcg tcgaaactgc tcggcggggc
     3001 gtcgatgttg gcctcggcga agagcttctt gttgtagacc atgagggtct tcgagaaata
     3061 atacggcagc atgtactgct tgccgtcgta ctgccagagc ttcatgccct cggcgttgta
     3121 gcgatcgcgg acctccgggt tggcctcgag ataaggatcg agcggcacca gcccgtcatt
     3181 ggcggcgtac tcggcccaca gcatgccttg gacgtcgatg atgtcggggg cggtgccggc
     3241 gaccagctgc gtctggtaga aggcgccgaa ctcggtgccc ttcttgtcca gccactcgac
     3301 cgtgaagtcc gggtgcatct cctcgaaatc ggcgatccag ccccggaact tctcctcgta
     3361 gatcggcagg ttccaggtca gcagcgtgag cttcttctcc tgcgccagcg ctgcctggcc
     3421 cgaaaggccg gcgagcagga gcgctccggc cactgtcgcc atgagatgtc gtctcagcat
     3481 cggtcaactc ctcccggttg gatgattgtc atgcgaagcg gagcggcact tcccgcccct
     3541 cccgcaccga tcgttcggcc tcgaggcaga cgaccaccgc gcgccggcct tcgcgcacgt
     3601 cgaccagcgt gcgtccctcg gcgaaggcgg cgttggtcag gccgatctgc ttctccagct
     3661 cgaagacctc gcccgagggc tcatcgagcg gcacgcgctc cggcgcctcg aggccgatcg
     3721 gtccgacatg caaatcgaag cagggctcgg tgctggcggc atggccggcc gaccaggtgg
     3781 cgcgcatggc cccttccgtc ccgctcacct cgaccaccag atggtggccg aatccggcga
     3841 gcgtctggct caccacggcg agtgctccgt catcgaagcg cacggccgag acgaggttgt
     3901 cgatgaggcc gggttcacgt cctgcggtgc cctgcgcatt cacggcgacc ggctcgccgg
     3961 aacgggccag ataccacatg gcgagatcat agaaatgcac cggctcctcg aggatccagg
     4021 agcccacctc gcccgccgac tgccgccagc cgctggcgcc cggccggtag gggtggcgaa
     4081 agagggcgac atgcacgaag gtcgggcggc cgatccgccc ggcgtcgatc agctccttga
     4141 tccgccgcca ctgcaccgag agccgaagct cgaagccgac cgagagcagc ccgcccgtgc
     4201 ggtccacggt ctccaccagc atgtcgcaat cggcgagcgt cgtcgccagc ggcttctcga
     4261 ccagcacggg cttgcccgcc tcgagcgccc gtttcgccat ggccgcgtgg aggtggttgg
     4321 gggcgacgat gtcgaccacg tcgattccgg gatcatcgac cagctttcgc cagtcggtcg
     4381 tcaccgccgc atccggatgg tggctgcggg cgagggcggc cgtggcctcg ctcgccgtcg
     4441 ccacggcggc gagctctgtg gcatcgccgc gggcgatcgc cgccgcgtgg agctggccga
     4501 aacggccata gccgatgacc ccccagcgaa gggttctggc accgctcatc gcagcgtcgg
     4561 ctcgggcaac tgcgccacct cgtcggtcag gaccgcccgg caatcggcgt ggcgctgcag
     4621 gagcgaggcc ggcacggcgg cggtgaccgg gccgtgcagc agcttgcgga cgatggcgga
     4681 ctgccagggg cggttgaggt agaggcggac caggcgggac gccaggatct cgcgcatgcc
     4741 gacagtcacg gcgagcctcg ggatccggtc gatattgcca cggcatgccg tgacgctgtt
     4801 gatcaccagt gtctcggtcg acagctcgac cacgcggctc ggcagggcgg cgaagctctc
     4861 ggtatcgatc gtctcgccgg gcgcaggcgg gtcgttgaag gcgacatggc cggtgatgcc
     4921 gaccccgcca tagcagacgt cgacgccacc gaaccgctcg atcgcaaggc ccacattggc
     4981 cggatcgatc gggtccggga agatgcgctg cccttcgggc ggtgccagat cgggcctgag
     5041 cacgtcccag aaatggcgct gcatgtggcc gcgaaagctg agcgggtcgc tcgccgcgat
     5101 gaaccttcgg ccatccggct cgagatactc gtccatgttg atgacgacga gatcggcaag
     5161 gctctcgcct gtccggttgc agtcctcggc cacgaggtcc agggcgccga ccggcccgac
     5221 gggcacgatg acgacgacct tctcccggcc ggcggccctg gcggcgcggt agtcggcaaa
     5281 taggtcgtcg gcgagccggc gaacgagggc cgcaccgtcg gcgacgcgct cgacccggat
     5341 cggcgaatcc cgacccaggg cctcggccgg cacggcgagg gcggcgtcgg tcgcctcatc
     5401 catggccggc tccttccagc cagagggcac ggccagacgc ggcatcgaag agatgcagct
     5461 tcgtcgggtc gacgtgcagc gtcagccgct cgggctggcc ctggacatgc cggggtggca
     5521 ggcgagccac cagctcggca ccgccgatcg agaagaagac gagcgtgtcg ctgccgagcg
     5581 gctcgatcac gtcgggctcg atgtcgagcg acggcccgcc gagcgtctcg gcatgaaagt
     5641 cctcgggccg gagccccatc aggaccggcc cgccgcgcgc ccccgaggcc atggccagct
     5701 gctcggccgg cagcggcaga agccggccgt ccggcagccg gagcacggct tccctggcgt
     5761 cgccgtcgat ctcgaccggc accaggttca tcgtcggcga gccgatgaag cccgccacgt
     5821 agctcgtccg gggatggttg tagacctcgt ccggcgtgcc gacctgctcg atcagcccgc
     5881 ccctcatgat gacgatgcga tccgccaggg tcatcgcctc gacctggtcg tgggtcacgt
     5941 agacgacggt ggtcgagacg cggcggtgca ggcgcttgat ctcggtgcgc atctggacac
     6001 gcagcttggc gtcgaggttc gagagcggct cgtcgaagag gaagacctgc gggtcgcgga
     6061 cgatggcccg ccccatggcg acgcgctgcc gctggccgcc cgacagctcc ttcggccgcc
     6121 gcagcagcag cggctcgatg tcgaggatgc gcgccgcatt ggcgacgcgc ttcttgatct
     6181 ccgccttggc gtatttgcga tagcggaggc cgaacgccat gttctcgtag accgacatgt
     6241 gcgggtagag cgcgtagtcc tggaaga
//
LOCUS       DAOTFI010000265         6267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14781_length_6267_cov_6.402930, whole genome shotgun sequence.
ACCESSION   DAOTFI010000265 DAOTFI010000000
VERSION     DAOTFI010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14781_length_6267_cov_6.402930"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..946
                     /locus_tag="P5558_20535"
     CDS             131..946
                     /locus_tag="P5558_20535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY26769.1"
                     /translation="MMRGARDRLIAPLLAAWTCLVLVLLYVPILSVVLASLANTRYMR
                     FPHKVWTLDAYRDALGQYTTGELHLTSFKIAALVAVLAVLLGVLGALAFARYDWRGRS
                     LYQRLLLLPIFFPQAVLGLALLLAMTAAGIAPSWRTAVFAHLVWVVPIVTLVISIRLY
                     GFDVAQEEAAFDLGASRLQVFREVTLPALAPGIVSGGLFAFLLSWSNLPLSIYTTGSD
                     TTLPLWLFSRTATNYSPLVPALAVIATGASVLVVVAAALALAGWRRLVARRSA"
     gene            complement(1013..1828)
                     /locus_tag="P5558_20540"
     CDS             complement(1013..1828)
                     /locus_tag="P5558_20540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY26770.1"
                     /translation="MRRALHPGTHLAIIGGGFIGLEVAASAVALGARASIIELALRLM
                     TRGVPEPLAARLADRHRNAGVALHLGRRIAAMAAVDDALRLTLDDGTVVAADHLLVAI
                     GASPRTELAVAAGLTVDGGIIVDEHLRTSDPATFAAGDCTAFPDPLTGDLIGRESRHN
                     AELQGPLAARNMLGAGEVLAPVPWFWSDQHEQTLQIAGTPEAGTRTVERATAHGLLLF
                     HLATDDRLVGVSALGPAALAKEFKVAQRLVEAGRRVEPRILADPATRLKALLR"
     gene            1930..3013
                     /locus_tag="P5558_20545"
                     /pseudo
     CDS             1930..3013
                     /locus_tag="P5558_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3005..3424)
                     /locus_tag="P5558_20550"
     CDS             complement(3005..3424)
                     /locus_tag="P5558_20550"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY26771.1"
                     /translation="MPGPIVIVGAGQAGVRAALALRDAGFDGPVTLLGAEPVPPYERP
                     PLSKGLLTGERDPENCTIASTEALAERRVAFHPATTAVAIDRDRRLVRSSDGGGHSFA
                     RLLLATGRRPRRLDLPPDLAPLAHELRHLEDARAFSR"
     gene            complement(3444..3758)
                     /locus_tag="P5558_20555"
     CDS             complement(3444..3758)
                     /locus_tag="P5558_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MocE family 2Fe-2S type ferredoxin"
                     /protein_id="HRY26772.1"
                     /translation="MPWTVVDDADAIEPEDVIRFDHAGRTYAVYRSADDAYFATDGLC
                     THEQVHLADGLVMGHVIECPKHNGRFDYRTGEPRRAPVRKALRTYPTKVEDGRILVDI
                     PD"
     gene            3894..4673
                     /locus_tag="P5558_20560"
     CDS             3894..4673
                     /locus_tag="P5558_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRY26773.1"
                     /translation="MARTSLRSWFYHQLDPLGRREHGLAPGNMLVASLILASCIVAVL
                     ETEPELYEGHGRLFAVCEWVFATLFLLEYGIRLWIAPESGRYGEGWRGVLRYAVTPVA
                     LLDLLAILPVFLLLAGTDAFILRIARLLRLLRLARLGRFSLAMHDMLEALMLRSYELG
                     LSLVIGFLLLLVSSTMLYLCEAAYQPDDFGSIPRAMWWSVSTLSTVGYGDAIPVTVPG
                     RIFAGLTAVGGIILIAMPAGILAAAFSDVQQRRRANPDRRL"
     gene            4840..5454
                     /gene="rpsD"
                     /locus_tag="P5558_20565"
     CDS             4840..5454
                     /gene="rpsD"
                     /locus_tag="P5558_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRY26774.1"
                     /translation="MTKRIQAKSKICRRLGVNLWGRTKSPLARRDYGPGEHGQKRRKM
                     SDFANQLAAKQKLKKYYANMSERQFRSAYEEASKTRGDTAENLIGLLERRLDITLFRF
                     NFVPTIFAARQFINHGHVKVNGKRVNIPSYRLREGDIIELREKAQNIPMVLEAVARPE
                     RDVPEYLAVDTKALKGQFVRVPKLSDVPYPVQMEPNLVIEYYSR"
     gene            5555..>6267
                     /locus_tag="P5558_20570"
     CDS             5555..>6267
                     /locus_tag="P5558_20570"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRY26775.1"
                     /translation="MRADCGSVANRDLAYTMSGGGVAFADLQLSLQCADGLARVAMTM
                     RNRGLAAFIAGDHRTDLTAEVEVGKGAPRPSHFSALYTKPDRTRETELTFAADGSLAD
                     LVVRHRGRERESPVSPALRDPSIDPLAALLQLSDWLAHDPAPDAAMALPVFDGRKRAD
                     LETVYRGRTTVEVAGKKRDAYHLTAALLGLSGFEEGDMLVTMPGQPPIWIDAYASIEP
                     MPMPLLIRSRDSAIESSIEL"
BASE COUNT          920 a         2187 c         2147 g         1013 t
ORIGIN      
        1 agcagcgctt cggctacgcc caggactggc cgtccggtgc cgctctcacc gtgctcgtca
       61 tcctggtcac cgccgccgtc atctttccgg tgctccgccg gctcgacctc gaccggctga
      121 tccggcgctg atgatgcgtg gcgcccgtga ccgcctgatc gccccgctcc tggccgcctg
      181 gacctgcctg gtgctggttc tcctctacgt gccgatcctc tccgtggtgc tcgcgtcgct
      241 cgccaacacc cgctacatgc gctttccgca caaggtctgg acgctcgatg cgtatcggga
      301 cgcgctcggg cagtacacca cgggcgagct gcatttgacc tcgttcaaga tcgcggccct
      361 ggtcgccgtc ctcgccgttc ttctgggtgt cctgggtgct ctcgccttcg cccgctacga
      421 ctggcgcggc cggagcctct accagcgcct gctgctgctg ccgatcttct tcccgcaggc
      481 ggtcctcggc ctcgccctcc tgctcgccat gacggccgcc gggatcgcgc cctcctggcg
      541 gaccgcggtc ttcgcgcatc tggtctgggt ggtgccgatc gtgacgctgg tgatctcgat
      601 ccggctctac ggcttcgacg tggcgcagga ggaggcggcc ttcgatctcg gcgcctcgcg
      661 gctgcaggtc tttcgcgagg tgaccctgcc ggcgctcgcg cccggcatcg tctcgggcgg
      721 gctcttcgcc ttcctgctct cctggtcgaa cctgccgctc tcgatctaca cgaccggctc
      781 cgacacgacg ctgcccttgt ggctcttctc gcgcacggcc accaactact cgcccttggt
      841 gccggcgctc gcggtgatcg ccacgggcgc ctcggtcctc gtggtcgtcg ccgcggcgct
      901 ggccctcgcc ggctggcggc ggctcgtggc ccgccgctca gcctgaggcg gcagcgctgc
      961 cgacggtgac gagcggagca acctaaagaa cgagccagat gcggcaagga cactagcgga
     1021 gcagcgcctt gagccgggtc gcgggatcgg cgaggatcct gggctcgacc cggcggcccg
     1081 cctcgaccag acgctgtgcc accttgaatt ccttggcgag cgccgccggc ccgagcgcgc
     1141 tcacgccgac cagccggtcg tcggtggcga gatggaagag gaggaggccg tgcgccgtcg
     1201 cgcgctcgac cgttcgcgtt cccgcttcgg gtgttccggc gatctgcagg gtctgctcgt
     1261 gctggtcgga ccagaaccag gggaccgggg cgagcacctc gccggcgccc agcatgttgc
     1321 gggcggcgag cggcccctgc agctcggcat tgtgccggga ttcccgaccg atcaggtcgc
     1381 cggtcagcgg atcggggaag gcggtgcagt cgccggcggc aaaggtggcg gggtcgctgg
     1441 tgcgcagatg ctcatcgacg atgatgccgc catcgacggt gagcccggcg gccaccgcga
     1501 gctccgtgcg ggggctggcg ccgatggcga ccagcaggtg gtcggccgcc accaccgtgc
     1561 cgtcgtcgag ggtgaggcgg agcgcgtcat cgacggccgc catggcggca atgcggcggc
     1621 caagatgcag cgcgacgccg gcgttgcggt gccgatcggc gagccgggcg gcgaggggct
     1681 cgggcacgcc ccgcgtcatg agccggaggg ccagctcgat gatgctggcc cgggcaccca
     1741 gggcgacagc gctggcggcc acctcgaggc cgatgaagcc gccgccgatg atcgccaggt
     1801 gcgtgccggg gtgaagcgcc cggcgcaggc gtttagagca ctttcaagct gaccggaatc
     1861 ttttcccgat tcccacttgt gacaggatgt gattcacact tcttggttgt ggtgatcggg
     1921 agaagcgtga tggcgtggcg gtcggggcag agctattcga cggatttgcg ggagcgggtt
     1981 ctggcggcga tggatggcgg gctgccggcg aggactgcgg cggagcggtt cgatgtcagt
     2041 gtttcgttcc tttacaaggc gttgaagcga cggcgggtgt ctggtgaggc gaccgcccgg
     2101 gcgcagcgca atcagcaggt gctgaagctc gcggcgcagc acgccgcgat cctggccgaa
     2161 gtcgagcggc ggtcggacgt gacgctggac gagttgcgcg cttggctgct cgagacgcat
     2221 ggcctcgagg cgagcctcgg cttgatgcac aacaccctga tccggctcgg cctgacgcta
     2281 aaaaaaaaga ccggacgggc ggaggagcag gatcgaccgg acatcgccga gcggcgcacc
     2341 gcttggcgcg ccgagcaggg tgcgctgagc cggggccggt cgatcttcac cgacgagacc
     2401 ggggcatcga ccaagctcgc tcgacgccac ggccgctgcc gccgtggcga acggctcgac
     2461 gcggcgctgc ccccctcgac ggcctcctgg ccgtcgtcat tccatgaatc ggatgattgg
     2521 cctcttggcc aatcatgggc actggaagac gacgaccttc gtcggcggac tgacaaaccg
     2581 tgggttcata gcaccttacg tgctcgacgg ggccatggac ggcgcgacgt tcagagcctg
     2641 gaccgagcag atgctcgccc cgaagccgga gcccggcgac atcgtcattc tggacaacct
     2701 cgccgcgcac aaggtccacg gtgtgcgcga ggcaatcgag ttcaggggcg ccgagctccg
     2761 ctatctgcca ctctattcac ccgatctcaa cccatcaaag gccaggaggc ctttgatccg
     2821 aaccatgaaa tggacgccgc cagcaggcgg cggaggcgag aacgccttcg ccaagctcaa
     2881 gccgctcctg agaaaggccg cccagcgcac catcgacgga ctttggacaa ccatcggcaa
     2941 gctgctcgac cgcttctcgc cggccgaatg ccaaaactac atcgaaaata ccggttatgt
     3001 acggtcagcg tgaaaatgct ctagcgtcct cgagatggcg cagctcgtgg gcgaggggcg
     3061 ccaagtcggg cggcagatcg aggcggcggg ggcggcgacc agtggcgagc agcaggcggg
     3121 caaaggaatg cccgccgcca tcgctcgagc ggacgaggcg gcggtcgcgg tcgatcgcca
     3181 ctgccgtcgt cgccgggtgg aaagcgacgc ggcgttcggc gagggcttcg gtggaggcga
     3241 tggtgcagtt ctccgggtcc cgctcgccgg tgagcagccc cttggagagg ggcgggcgct
     3301 catagggtgg cacgggctcc gcgccgagca gcgtcaccgg gccgtcgaag ccggcatcgc
     3361 gcagggccag cgccgcccgc accccggcct ggccggcgcc gacgatgacg atcgggcccg
     3421 gcatgtcagg cgggccactc acgtcagtcg gggatgtcga cgaggatgcg gccgtcctcg
     3481 acctttgtcg ggtaggtccg cagggctttg cgcaccggtg cccgcctggg ctcgccggtg
     3541 cggtagtcga agcggccgtt gtgcttgggg cactcgatga cgtggcccat caccaggccg
     3601 tcggcgagat gcacctgctc gtgggtgcag aggccgtcgg tggcgaagta ggcgtcgtcg
     3661 gccgagcggt agacggcata ggtgcggccc gcgtggtcga agcggatgac gtcctcgggc
     3721 tcgatcgcgt cggcgtcgtc gacaactgtc cagggcatgg tccggtctcg cggcaggggt
     3781 gcggggcggc atcgaagcgc aagtgccgcc cggcgtaaac cgccggatgc tcgcaagccg
     3841 cggccccggc tggtagcaag ggcgcgtctc gttgcacagc cggtagctca gccatggccc
     3901 gcaccagcct ccgctcctgg ttctatcacc agctcgatcc gctcggccgg cgcgagcacg
     3961 ggctggcacc gggcaacatg ctggtcgcca gcctgatcct ggcgtcctgc atcgtggcgg
     4021 tgctggaaac cgagccggag ctctacgagg gccacggtcg gctcttcgcc gtatgtgaat
     4081 gggtgttcgc gacgctcttt ctgctggagt acggcatccg cctctggatc gcaccggaga
     4141 gcggccgcta tggcgaaggc tggcgcggcg tgctgcgcta cgccgtaacg ccggtggcgc
     4201 tgctcgatct gctggcgatc ctgccggtct tcctcctgct ggccgggaca gacgccttca
     4261 tcctgcggat cgcccggctg ctgcggctct tgcggctggc caggctcggc cgcttctcgc
     4321 tcgccatgca cgacatgctc gaggccctga tgctgcgcag ctacgagctc gggctcagcc
     4381 tggtcatcgg ctttctcctg ctgctggtca gctcgaccat gctctatctc tgcgaggcgg
     4441 cctaccagcc agacgatttc ggctcgatcc cgcgggcgat gtggtggtcg gtctccaccc
     4501 tctccacggt cggctatggc gacgccattc cggtgaccgt gcccggccgc atcttcgccg
     4561 gcctcaccgc ggtcggcggc atcatcctca tcgccatgcc ggcgggcatc ctggccgccg
     4621 ccttcagcga cgtgcagcag cgccgccgcg ccaacccgga ccgccggctc tgagcgatgc
     4681 gcgaaacgac gcaaagcctt tgacaagccc gggtgcgcgg actagaagct cgccatcggc
     4741 cgggggcctt ccccggctgc gttgctttgt gcgggccacg atccgttgcc ggcgccggaa
     4801 agcgacgttc ccttacaccg cttctgcaaa tcggggccga tgaccaagcg tatccaggct
     4861 aagtccaaga tctgccgccg cctcggggtg aatctctggg gccgcaccaa gagtcctttg
     4921 gcccggcgcg actacggccc gggcgagcac ggccagaagc gacgcaagat gtccgacttc
     4981 gccaaccagc tcgccgccaa gcagaagctg aagaagtact acgccaacat gagcgagcgg
     5041 cagttccgca gcgcctacga ggaagccagc aagacccgcg gcgacaccgc cgagaacctg
     5101 atcgggctgc tcgagcggcg cctcgacatc acgctcttcc gcttcaactt cgtgccgacc
     5161 atctttgccg cccggcagtt catcaatcac ggccatgtga aggtgaacgg caagcgggtc
     5221 aacatcccgt cctaccgcct gcgcgagggc gacatcatcg agctgcgcga gaaggcgcag
     5281 aacatcccga tggtgctgga agccgtggcc cgccccgagc gcgacgtgcc ggaatacctc
     5341 gccgtcgaca ccaaggcgct gaagggccag ttcgtccgcg tgccgaagct ctcggacgtg
     5401 ccctatcccg tgcagatgga gccgaacctc gtcatcgaat actactcgcg ctgacgaacc
     5461 ccgggcgccg ctccgggcag ggtcctccgg gccctcgccg aggacgcggc gctcgctcct
     5521 catcggtttc ggcctgacac tcgtcgccgg tgccgtgcgc gccgactgcg gcagcgtcgc
     5581 caaccgcgac ctcgcctaca ccatgagcgg cggtggcgtt gccttcgccg atctgcagct
     5641 cagcctgcaa tgcgcggacg gcctggcgcg tgtcgccatg acgatgcgca atcgcggcct
     5701 cgccgccttc atcgccggcg atcaccgcac cgatctgacc gccgaggtcg aggtcggcaa
     5761 gggtgccccc cgtccgtcgc atttcagcgc cctctacacc aagcccgacc ggacgcgcga
     5821 gaccgagctc accttcgccg ccgacggcag cctcgcggac ctcgtcgtcc gccatcgcgg
     5881 ccgcgagcgc gagagcccgg tgtcgccggc cttgcgcgat ccctcgatcg accccctggc
     5941 ggccctgctc cagttgagcg actggctggc ccacgacccc gccccggacg cggccatggc
     6001 cctgccggtc ttcgacggcc gcaagcgcgc cgacctcgag accgtctatc ggggccgcac
     6061 cacggtcgag gtcgcgggca aaaagcgcga cgcctaccac ctgaccgcgg cgctcctcgg
     6121 cctctcgggc ttcgaggagg gcgacatgct ggtcacgatg ccgggccagc cgcccatctg
     6181 gatcgacgcc tatgccagca tcgagcccat gccgatgccg ctgctcatcc gctccaggga
     6241 ttccgcgatc gagagctcga tcgagct
//
LOCUS       DAOTFI010000266         6259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_14812_length_6259_cov_7.296260, whole genome shotgun sequence.
ACCESSION   DAOTFI010000266 DAOTFI010000000
VERSION     DAOTFI010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14812_length_6259_cov_7.296260"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="P5558_20575"
     CDS             <1..977
                     /locus_tag="P5558_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26776.1"
                     /translation="DAFLLPFQAPVSEVPLVVIEAGLTGRPVITLEAPGVSEQVLALG
                     GFVARTPEELPHALIRALRQRQDMLAVDPGSWTSWPRAVSAMLDAPVPPSLPQRLIAL
                     CGVDGTGKSFLCEHLRRHFAEAGIANRHVWSRFRNYLSKPLLALARLTGHNYKVETGG
                     VRIGYHDFVGRPWLVWPFLALQAIDQVLDILWRYHLGRREPILGDRCVLDTLVDLAID
                     TGLDDVVIDRLGPLLVALLPRPRLVVLIERHPLRIQEQRPDALLDRHFHRRRLLYRRL
                     AERFDLPVVDNNGPVARTLEAILALVQEQNSGIGAGMAPTAAAERTGQ"
     gene            974..2086
                     /locus_tag="P5558_20580"
     CDS             974..2086
                     /locus_tag="P5558_20580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY26777.1"
                     /translation="MIYVFYNNDPLAQDQGGGAEHFRGLHRALLKSGLEFHLVAARLQ
                     QGRAAQHITYVSESSNFLRFFCGLWVWFWRNRKTFRAADVFHFHRNYAAWPKLIICPE
                     QGRVLISYHNVSGRVLEGWLGRIGRPLRRAMLLLERRAVRLADAIICVSGRDRESLQE
                     AVAPAPFAAASVIPAAFDDRIFADRPASPPGPALAHRLVLLGRISHQKNVPLAVATLE
                     ELVARGRAFTLTIVGEGEDSPDLIRRIARSPARERLTWVGRVPHDQVPQLLADHGILL
                     LTSRYEASPTVVKEALKAMRPVVSTDVGDVADWLDEGQSGFLRDPDPKALADAVEAAA
                     CLIHEGGYRQGSRVDRLDEDSIMSEVVHLYRRLQAV"
     gene            2083..3375
                     /locus_tag="P5558_20585"
     CDS             2083..3375
                     /locus_tag="P5558_20585"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRY26778.1"
                     /translation="MIAALGRGFRAVLPPAALGRLVAMGGSRALAAGLGLIATILIAR
                     GLEPAGLGLWSMALAVQGIALHLGEAGLRSIVTVEMAARPEAGRALLYRYLGLRLAIS
                     TGVIGLAVAGARLVGIADWPYLALLLTSLWPIALQLDWLPLAHGQTGQAGLLLLIRPV
                     VFVLLLLALPLAGDPERLALLFLAAWWVAAAATWPWLRLVRQAPVRAAAPGRRRLLRL
                     ALPVAVATVASQLLLSLDLLLVGARFGPAVAAQYYLASAILVAGLVVANGLGQAALAR
                     LAAATGTGFRAALEADLRLVGAVAALAAVTVLGVVPHLLPPAFGAAYAPAASLLVWLL
                     PWFVLQHATAVLQAGMTAARLGDRQLRAALWLVAALVPGLALAWWFGDLRLFALARGL
                     AELVRVVALWRGLPPALRPLSARHPSFGRRRRRRRGAR"
     gene            complement(3317..6049)
                     /gene="polA"
                     /locus_tag="P5558_20590"
     CDS             complement(3317..6049)
                     /gene="polA"
                     /locus_tag="P5558_20590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRY26779.1"
                     /translation="MSEPTKPRVVLVDGSGYIFRAFFALPPMTRGDGTPVNAVFGFSS
                     MLFKLMQDHADDDMIVVFDAGRVSFRNEIYEAYKANRDEPPPELVPQFALVRDAARAF
                     GLPAVEVPGFEADDLIATYVDRARALDREVVIVSSDKDLMQLVAPGVVMWDPMKSRMI
                     GPAEVEERFGVGPELVQDCLALAGDSSDNVPGVPGIGVKTAAQLLNQFGSLDELLANA
                     ATIKQPKRRQTLIDFAEQARLSKRLVALRHDAPLPIELDGSVRGQIDGATLLAFLQAN
                     DFRALIGRIEQVADAAEAERAALARDQGTDGPVWRTITDLAELDALLAEATAIGRLAI
                     DTETTSLNVAKAELVGVSLSLEPGRGAYVPLGHVDDFGQRLQGQPPLAQVLERLRPVL
                     ADRGTLKIGHNLKYDMAVLARYEVPITPFDDTMLLSYVLDGASHGHGMDELARRHLGH
                     ETISYESVCGKGAKQIGFAQVPIEKATPYAAEDAEVTLRLWQLLKRRLIDERVTTVYE
                     SLERPLPPIVAAMEVAGIQVDRHHLEKLSAEFGQRMAELEEAAVKLAGRPFNLGSPRQ
                     LGEILFDEMGIVGAKKTKTGAHATGADILEDLAAQGHELPKVILDWRQLQKLTRTYTE
                     SLVEEIVPGTGRVHTSYMLAATSTGRLSSTDPNLQNIPIRTEEGRKIREAFVPAEGHA
                     LVSADYSQIELRVLAHMADIQALKDAFAHDTDIHAVTAGKMFKLPVEEVSSELRRRAK
                     MINYGIIYGIGAFGLAQRLAIPQAEAKAFIEAYFAEYPGVRDYMERAKEEAREQGHVL
                     TLFGRRCFIPEILSKQPARRAYAERQAINAPIQGSAADIMKRAMRRVARAIDRDGLEA
                     RLLLSVHDELVFEVPEDQVEPARDVIRTVMEGAAELTVPLVVDVGAGRSWGAAH"
BASE COUNT          781 a         2220 c         2251 g         1007 t
ORIGIN      
        1 tcgacgcgtt cctcctgccg ttccaggcgc cggtctcgga ggtgccgctc gtggtcatcg
       61 aggcggggct caccggccgg ccggtgatca cgctcgaggc accaggcgtc agcgagcagg
      121 tgctggccct gggcggcttc gtcgcccgga cgccggagga gctgccgcac gcgctgatcc
      181 gcgccctgcg gcagcggcag gacatgctcg cggtcgaccc gggctcgtgg acctcctggc
      241 cgcgcgcggt ctcggccatg ctggacgccc cggtgccgcc gtccctgccc cagcggctga
      301 tcgccctctg cggtgtcgac ggcaccggca agagcttcct ttgcgagcat ctgcgccggc
      361 atttcgccga ggcggggatc gcaaaccggc acgtctggag ccggtttcgc aactatctct
      421 cgaagccgct cctggctctg gcccggctca ccggccacaa ctacaaggtc gagaccggcg
      481 gggtgcgaat cggctaccac gatttcgtcg gccggccctg gctcgtctgg cccttcctgg
      541 cgctgcaggc gatcgaccag gtgctcgaca tcctctggcg ctaccatctc gggcggcgtg
      601 agcccattct gggcgaccgc tgcgtcctcg acacgctggt cgacctcgcg atcgacacgg
      661 gcctcgacga tgtcgtcatc gaccggctcg ggccgctgct cgtggcgctg ctgccgcgac
      721 cgcgcctggt cgtgctgatc gagcggcacc cgctgcgcat ccaggagcag cggccggacg
      781 ccctgctcga tcgccatttc catcgccgac ggctgctcta tcgccgtctc gccgagcgct
      841 tcgacctgcc ggtcgtcgac aacaacgggc cggtggcgcg cacgctcgag gccatcctgg
      901 cgctggtgca ggagcagaat tcgggcatcg gggcgggcat ggcgccgact gccgcagccg
      961 agaggaccgg ccagtgatct acgtcttcta caacaacgat ccgctggccc aggaccaggg
     1021 cggcggggcc gagcatttcc gtggcctgca tcgagcactc ctcaagtcgg gcctcgagtt
     1081 ccacctggtc gccgcccgcc tgcagcaggg acgggccgcc cagcacatca cctatgtgtc
     1141 ggagagctcg aacttcctgc gcttcttctg cggcctctgg gtctggttct ggcgcaaccg
     1201 caagacgttc cgcgccgccg acgtctttca tttccaccgt aattacgcgg cctggcccaa
     1261 gctcatcatc tgcccggagc agggtcgcgt gctgatcagc taccacaatg tcagcggccg
     1321 ggtgctggaa ggctggctcg gccggatcgg caggccactc aggcgggcca tgctgctgct
     1381 cgagcggcgt gccgtgcggc tggcggacgc catcatctgc gtcagcggcc gcgaccgcga
     1441 atcgctgcag gaggcggtgg caccggcgcc ctttgcggcc gccagcgtca tcccggcagc
     1501 cttcgacgac cggatcttcg ccgatcgccc cgcttccccg cccggcccgg ccctcgccca
     1561 tcgcctcgtc ctgctcggcc ggatcagcca ccagaagaac gtgccgctcg ccgtcgccac
     1621 gctcgaggag ctggtggccc gcggccgcgc gttcacgctc accatcgtcg gcgagggcga
     1681 ggacagcccg gacctgattc gccgcatcgc ccgctcgccg gccagggagc gcctgacctg
     1741 ggtcggccgg gtgccgcacg accaggtgcc gcaactgctg gccgaccacg gcatcctcct
     1801 gctcaccagc cgctacgagg cctcgcccac ggtggtcaag gaggcgctga aggcgatgcg
     1861 cccggtcgtc tcgaccgatg tcggcgacgt cgccgactgg ctggacgagg gccagtcggg
     1921 cttcctccgc gatcccgatc ccaaggcgct cgccgatgcg gtcgaggccg ccgcctgcct
     1981 catccacgag ggtggctacc ggcagggcag ccgggtcgac cggctcgacg aggattcgat
     2041 catgagcgag gtggtccacc tctaccggcg gctccaggcc gtttgatcgc cgcgctcggc
     2101 cggggctttc gggcggtcct gccgcccgcg gcgctcggcc gcctcgtcgc catgggcggc
     2161 agccgggcgc tggcggccgg cctcggcctg atcgccacga tcctgatcgc ccggggcctc
     2221 gaaccggcag gcctcggcct ctggtcgatg gcgctggcgg tccagggcat cgccctgcat
     2281 ctgggcgagg cggggctgcg ctcgatcgtg acggtcgaga tggcggcccg gcccgaggcc
     2341 ggacgggcgc tgctttaccg ctatctcggc ctcaggctcg ccatcagcac gggcgtgatc
     2401 gggctcgcgg tcgccggcgc ccggctcgtc ggcatcgccg actggcccta tctggcgctg
     2461 ctgctcacca gcctctggcc catcgcgctg cagctcgact ggctgccgct cgcccatggc
     2521 cagacgggcc aggcggggct cctgctcctg atccgcccgg tcgtcttcgt cctcctgctg
     2581 ctcgccttgc cgctcgccgg cgacccggag cgcctggccc tgctcttcct cgccgcctgg
     2641 tgggtggcgg cggcggccac ctggccctgg ctacggctgg tccggcaggc gccggtgcgg
     2701 gctgccgccc cgggccggcg ccggctgctg cgcctcgcct tgccggtggc ggtggcgacg
     2761 gtggcgagcc agcttctcct gagcctcgac ctgctcctcg tcggtgcgcg gttcgggccg
     2821 gccgtggcgg cgcagtacta cctcgcctcg gcgatcctgg tggccgggct cgtcgtcgcc
     2881 aatggcctcg gccaggccgc cctcgctcgt ctggcggcgg caaccgggac cggctttcgg
     2941 gcagcgctcg aggccgacct ccggctggtc ggcgccgtcg cagcactcgc cgccgtcact
     3001 gtgctcggcg tcgtgccgca cctcctcccg cccgccttcg gcgccgccta tgcgcccgcc
     3061 gcgagcctgc tcgtctggct cctgccctgg ttcgtcctgc agcatgcgac ggcggtgctg
     3121 caggcgggga tgaccgcggc ccggctcggc gaccggcagc ttcgggcggc actctggctg
     3181 gtcgcagcac tcgtgccggg gctggcgctc gcctggtggt tcggggatct ccgcctcttc
     3241 gccctcgccc gcggcctggc cgagctggtc cgggtggtgg cgctctggcg cggcctgccg
     3301 ccggcgctgc ggccgctcag tgcgcggcac cccagcttcg gccggcgccg acgtcgacga
     3361 cgaggggcac ggtgagctcg gcagcaccct ccatgaccgt ccggatgaca tcccgggctg
     3421 gctcgacctg gtcctcgggc acctcgaaga ccagctcgtc gtggaccgag agcaggagcc
     3481 gggcctcgag cccgtcacgg tcgatggcgc gcgcgacgcg gcgcatggcg cgcttcatga
     3541 tgtcggcggc ggagccctgg atgggggcgt tgatggcctg gcgctcggcg taggcccgcc
     3601 gcgccggctg cttggagagg atctccggga tgaagcagcg gcggccgaag agggtgagga
     3661 cgtggccctg ctcgcgagcc tcctccttgg cccgctccat gtagtccctg acacccggat
     3721 actcggcgaa ataggcctcg atgaaggcct tggcctcggc ctgcgggatg gcgaggcgct
     3781 gggcgaggcc gaaggcgccg atgccgtaga tgatgccgta attgatcatc ttggcccgcc
     3841 ggcgcagctc gctcgacacc tcctccacgg gcagcttgaa catcttgccg gcggtcacgg
     3901 cgtggatgtc ggtgtcgtgg gcgaaggcgt ccttgagcgc ctggatgtcg gccatgtggg
     3961 cgaggacgcg cagctcgatc tgcgagtagt cggcggagac cagggcgtgg ccttccgccg
     4021 gcacgaaggc ctcgcggatc ttcctgcctt cctcggtgcg gatcgggatg ttctgcaggt
     4081 tggggtcggt cgaggagagc cggccggtcg aggtggcggc cagcatgtag gaggtgtgca
     4141 cccggccggt cccgggcacg atctcctcga ccagcgattc ggtgtaggtg cgggtgagct
     4201 tctggagctg gcgccagtcg aggatcacct tgggcagctc gtggccctgg gccgccaggt
     4261 cctcgaggat gtccgcgccc gtggcatggg cgccggtctt ggtcttcttc gccccaacga
     4321 tgcccatctc gtcgaacagg atctcgccga gctgcctggg cgagccgagg ttgaagggcc
     4381 ggccggcgag cttgacggct gcctcctcga gctcggccat gcgctggccg aactcggcgg
     4441 agagcttctc cagatgatgc cggtcgacct ggatgccggc cacctccatg gccgccacga
     4501 tgggcggcag cgggcgctcg aggctctcgt agacggtcgt cacgcgctcg tcgatcagcc
     4561 gccgcttgag gagctgccag aggcggagcg tcacctcggc gtcctcggcg gcatagggtg
     4621 tggccttctc gatcggcacc tgcgcgaagc cgatctgctt cgcccccttg ccgcagacgg
     4681 actcgtagga gatcgtctcg tggccgagat ggcgccgggc cagctcgtcc atgccgtggc
     4741 cgtggctggc gccgtcgagc acgtaggaga gcagcatggt gtcgtcgaag ggggtgatcg
     4801 gcacctcgta cctggccagc accgccatgt cgtatttgag gttgtggccg atcttgagcg
     4861 tgccccggtc ggcgaggacc gggcgcagcc gctcgagcac ttgggcgagc ggcggctgac
     4921 cctggagccg ctggccgaaa tcgtcgacat ggccaagggg gacataggcg ccccggcccg
     4981 gctcgaggct gagcgagacg ccgaccagct cggccttcgc gacgttgagc gaggtggtct
     5041 cggtgtcgat ggcgaggcgg ccgatggcgg tcgcctcggc gaggagggcg tcgagctcgg
     5101 cgaggtcggt gatcgtccgc cagaccgggc catccgtgcc ctggtcgcgg gcgagggccg
     5161 cgcgctccgc ctcggccgca tcggcgacct gctcgatccg gccgatcagc gcgcgaaaat
     5221 cgttggcctg caggaaggcg agcagcgtgg ccccgtcgat ctgccccctg accgagccgt
     5281 cgagctcgat tggcaggggc gcgtcgtggc gcagcgccac gagccgcttc gagaggcggg
     5341 cctgctcggc gaagtcgatc agggtctggc gccgcttggg ctgcttgatc gtcgcggcat
     5401 tggccagcag ctcgtcgagc gagccgaact ggttcaggag ctgggctgcg gtcttgacgc
     5461 cgatgcccgg aacgccgggg acgttgtcgg agctgtcgcc ggccagcgcc aggcagtcct
     5521 ggacgagctc ggggccgacg ccgaagcgct cctcgacctc ggccgggccg atcatccggc
     5581 tcttcatcgg gtcccacatg acgacacccg gggccacgag ctgcatcagg tccttgtcgg
     5641 aggagacgat gaccacctcg cgatcgagcg ctcgcgcccg gtcgacatag gtggcgatca
     5701 ggtcgtcggc ctcgaagccc ggcacctcga cggcgggcag gccgaaggcg cgcgccgcgt
     5761 cgcggaccag ggcgaactgc ggcaccagct ccggcggcgg ctcgtcgcga ttggccttgt
     5821 aggcctcgta gatctcgttc ctgaagctga cccggccggc atcgaagacg acgatcatgt
     5881 cgtcgtcggc atggtcctgc atcagcttga agagcatcga cgagaagccg aagacggcat
     5941 tgaccggcgt gccgtcgccg cgggtcatcg gcggcagggc gaagaaggcg cggaagatgt
     6001 agccggagcc gtcgacgagg acgacgcggg gcttcgttgg ctcgctcatg gtgcgtctct
     6061 agcgcactgg cgtccgccag cctaggagcc tgtccgagca atagccaccg cctgcatcgc
     6121 tctctggcgg gcgagggcaa ggagcggcga cgatggcgat gccacagcat cggcaaggcg
     6181 gcgtgacgcg ggcctcgccc gccagagagc gacccgaagg gcgcgccctt cctggccttg
     6241 ggccacgtcg accggccct
//
LOCUS       DAOTFI010000267         6174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_15071_length_6174_cov_7.447132, whole genome shotgun sequence.
ACCESSION   DAOTFI010000267 DAOTFI010000000
VERSION     DAOTFI010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15071_length_6174_cov_7.447132"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="P5558_20595"
     CDS             complement(<1..778)
                     /locus_tag="P5558_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY26780.1"
                     /translation="MAEAYPITEHVFDAIVVGAGGSGLRAAVGMVEAGLSVACVTKVY
                     PTRSHTVAAQGGINAALGNMNEDDWRFHMYDTVKGSDWLGDQDAIEYMTKNAPAAVYE
                     LEHYGVPFSRTDAGRIYQRAFGGQSVDFGKGQAYRTCAAADRTGHVILHTLFQQALKN
                     KVQFFNEYFALDLLMGEDGECLGILAMCIEDGSLHRFRAHQTCLATGGYGRVYFSATS
                     AHTCTGDGNGMALRAGLPLQDMEFVQFHPTGIYGAGCLITE"
     gene            complement(783..1157)
                     /gene="sdhD"
                     /locus_tag="P5558_20600"
     CDS             complement(783..1157)
                     /gene="sdhD"
                     /locus_tag="P5558_20600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HRY26781.1"
                     /translation="MQAAIGKVRSRGSARSGAGTWKAQRFTAIANLLLVLWFVVNAIA
                     MAGSAYAEWVAWFHSPLTAALMILLVLSTCYHARIGLQEVVEDYVHHEIGKVAILVGI
                     TLVAALLATAGVVSVLMIALGG"
     gene            complement(1180..1566)
                     /gene="sdhC"
                     /locus_tag="P5558_20605"
     CDS             complement(1180..1566)
                     /gene="sdhC"
                     /locus_tag="P5558_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HRY26782.1"
                     /translation="MAQGARPLSPHLQIYRWYLTMALSIGHRASGVALTVGLVLFIWW
                     LVALAGGPESFAVVQAVMHNFFGWLVLFGFTAALFFHAANGVRHLVWDAGYGFDKEVA
                     RQSAIVTLAVAGGLTILFWLLVLIVA"
     gene            complement(1711..2475)
                     /locus_tag="P5558_20610"
     CDS             complement(1711..2475)
                     /locus_tag="P5558_20610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY26783.1"
                     /translation="MVENEALIRQIEEAAWRAWPAARTALLAGWRLRAGPTATRRENS
                     VQAMSFTGELAPDAAIRSAEAWYAAAGKPACFQLNALSAPLGLDAALAGRGYRAEGRS
                     SVLARPLDVLPEEPAQVVLEGRATAFAMQAVADPRWPAAERAARAALFARIRRPLAFA
                     VRTVGGEPVAGGLCVFDGPLAGLAAMRTATTARRQGHGRAVLDRLLVWAGRMGAGTAW
                     LQVEADNAPAAALYRSAGFLPLYDYHYRVAGAAGTG"
     gene            2526..6140
                     /locus_tag="P5558_20615"
     CDS             2526..6140
                     /locus_tag="P5558_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRY26784.1"
                     /translation="MPTGWQFWIDRGGTFTDIVARQPDGGIRIHKLLSENPGQYADAA
                     LQGIRDVLGLGRDEPLPVAEIDAIKMGTTVATNALLERKGTPTVLAITRGLGDCLKIG
                     YQHRPDIFARHIVLPEQLYSRVVEIDERLLADGAVDRPLDEARSRADLQAAFDEGFRA
                     VAIVLMHGYRHPDHESRVAELAAGIGFTRISVSHQVSPLMKIVGRGDTTVVDAYLSPI
                     LRRYVDRVAARTEGVRLMFMQSNGGLVDAHRFQGKDAILSGPAGGIVGAARTAAIAGF
                     ERIIGFDMGGTSTDVTHYAGEYERAFETEVAGVRMRAPMMKIHTVAAGGGSIITFDGQ
                     RFRVGPESAGADPGPACYRKGGPLAVTDANLMVGKLLPDFFPHVFGPAADQPLDEAVV
                     RERFAALAAEASVATGVERRPEEIAQGCLAIANDNMANAIKEISIARGIDVQGYTLVC
                     FGGAGGQHACQVADLLGIGSVQIHPYAAVLSAYGMGLADLRTIRQRALDTALEPGIEG
                     ELEAARAELACSAAAELAEQGIEPEALRLEARAHIRYAGTDTPIEVPLGTPDAMRAAF
                     EAEHKSRYGFTVEGRGLVVETLTVEGIGLMQAIDEPEVQVAADRDPPAPVGKAHMFTT
                     SAPHQPAETFEAAVWRREDLARGDRVRGPAIVAGSTTTIVVEPGWALEVTPRDHVVLT
                     RVEKLPERVALGTHCDPILLEIFNNLFMTIAEQMGVTLRNTAYSVNVKERLDFSCAVF
                     DETGGLIANAPHMPVHLGSMGESVRTVIRLRRDTMGPGDVYALNNPYNGGTHLPDVTV
                     VMPVFGEAGEVLFYTASRAHHADIGGITPGSMPPFSRHIDEEGVLFDDFHLVEAGRFR
                     HDAVEALLKSGPHPARNPRQNIADLQAQVASCAKGAAELDRMVGQFGIETVRAYMRHV
                     MDNAEESVRRVLDRLQDGAFTYAHDRGPVIEVKVTVDRAGRSARIDFTGTSPQQDWNF
                     NAPAAVTRAAVLYVFRTLVDSDIPLNDGCLKPLELVIPEGSILACKHPAAVCAGNVET
                     SQWCTDTLFGALGALAAAQGTMNNFTFGNATHQYYETICGGSGAGPDHPGTSAVHTHM
                     TNSRLTDPEILEWRYPVLLEEHRIRRGSGGKGRFAGGDGTLRRIRFLEPMAAALLANH
                     RKVPPFGLDGGEPGQCGDQWVERRNGKVEKLEGCDATELEAGDVFAIQTPTGGGYGKA
                     "
BASE COUNT          977 a         2242 c         2118 g          837 t
ORIGIN      
        1 cctcggtgat caggcagccg gcgccataga tgccggtggg gtggaactgc acgaactcca
       61 tgtcctgcag cggcaggccg gcccgcagcg ccatgccatt gccgtcgccg gtgcaggtgt
      121 gcgccgaggt ggcggagaag tagacgcggc cataaccgcc ggtggcgagg caggtctggt
      181 gcgccctaaa gcggtggagc gagccgtcct cgatgcacat ggcgaggatg ccgaggcact
      241 cgccgtcctc gcccatcagg aggtcgaggg cgaaatactc gttgaagaac tgcaccttgt
      301 tcttgagcgc ctgctggaac agcgtgtgca ggatgacgtg gccggtgcgg tcggcggcgg
      361 cacaggtgcg gtaggcctgt cccttgccga aatcgacgga ctggccgccg aaggcgcgct
      421 ggtagatgcg gccggcgtcg gtgcgggaga agggcacgcc gtaatgctcg agctcgtaga
      481 ccgcggccgg cgcgttcttg gtcatgtact cgatcgcgtc ctggtcgccg agccagtcgg
      541 agcccttgac ggtgtcgtac atgtggaagc gccagtcgtc ctcgttcatg ttgccgaggg
      601 cggcgttgat cccgccctgc gccgccacgg tgtggctcct ggtcgggtag accttggtga
      661 cgcaggcgac cgagaggccg gcctcgacca tgccgacggc ggccctgagg ccggagccgc
      721 cggcgccgac gacgatggca tcgaacacat gctcggtgat ggggtaggcc tcggccatcg
      781 gatcagcctc caagcgcgat catgaggacg gagacgacac cggccgtggc gagcagggcc
      841 gcgacgagcg tgatgccgac gaggatggcg accttgccga tctcgtgatg gacatagtcc
      901 tcgaccacct cctgcagccc gatcctggcg tggtagcagg tcgagagcac caagaggatc
      961 atcagggcgg cggtcagggg cgagtggaac caggcgaccc attcggcgta ggccgagccc
     1021 gccatggcga tggcgttgac gacgaaccag agcaccagga ggagattggc gatggcggtg
     1081 aagcgctgcg ccttccaggt gccggcgccc gagcgcgccg agccccgact gcggaccttg
     1141 ccgatagcgg cttgcatgga atgtcctttc cctggaggct caggcgacga tcagcacgag
     1201 cagccagaag aggatggtca gcccgcccgc caccgccagg gtgacgatgg cgctctgccg
     1261 ggccacctcc ttgtcgaagc cgtagccggc gtcccagacg agatggcgaa cgccgttcgc
     1321 ggcatggaag aagagggcgg cggtgaagcc gaagagcacg agccagccga agaagttgtg
     1381 catcaccgcc tgcaccacgg cgaagctctc cgggccgccg gcgagggcga ccagccacca
     1441 gatgaagagc acgagcccga ctgtcagcgc caccccgctg gcacggtgcc cgatcgacag
     1501 cgccatggtg agataccagc gatagatctg gaggtgcggg gagagtggtc gagcgccctg
     1561 tgccatgccg gctccgtttc ctgacccgag cagccgccct gccggcggcc gatttgtgcg
     1621 atgcgtcata ggctgaggcg atactagaag cgaccggaaa cctgtcaacg cgacagaacc
     1681 acccgccggc gcaaggcccg gcccctcgcc tcaccctgtg cccgccgccc cggccacgcg
     1741 atagtgatag tcgtagaggg gcaggaagcc ggcgctgcgg tagagggccg ccgccggcgc
     1801 gttgtccgcc tcgacctgca gccaggccgt tccggccccc atccggccgg cccagacgag
     1861 cagccggtcg agcacggccc ggccgtggcc ctggcggcgc gccgtggtcg ccgtgcgcat
     1921 tgcggctaac ccggcgagcg ggccgtcgaa gacgcagagc ccgcccgcca ccggctcgcc
     1981 gcccaccgtc ctgacggcga aggccagcgg ccggcggatg cgggcgaaga gcgctgcccg
     2041 ggccgcccgc tcggccgcgg gccagcgcgg gtccgccacc gcctgcatgg cgaaggcggt
     2101 ggcccggccc tcgagcacga cttgcgccgg ctcctcgggc aggacgtcga gggggcgcgc
     2161 aagcaccgag ctccggccct cggcccggta gccgcgcccg gccagggccg cgtcgagccc
     2221 gaggggcgcg ctcagcgcgt tgagctggaa gcaggccggc ttgccggccg ccgcgtacca
     2281 ggcctcggcc gaccggatcg cggcatccgg cgccagctcg ccggtgaagc tcatcgcctg
     2341 caccgaattc tcgcgccgcg tcgcggtcgg tccggcacgc agccgccaac ctgcgaggag
     2401 cgccgtacgt gccgccggcc aagcccgcca cgcggcttcc tcgatttgcc ggatcagagc
     2461 ctcgttctcg accatgccct cctctatagc gataaccgct ggagcaagag agagggcagg
     2521 ggatcatgcc gaccggctgg cagttctgga tcgatcgcgg cggcaccttc accgacatcg
     2581 tcgcaaggca gccggatggc ggcatccgca tccacaagct gctctccgag aaccccgggc
     2641 aatacgcgga tgcggcgctg cagggcattc gcgacgtgct cggcctcggc cgggacgagc
     2701 cgctgccggt ggcggaaatc gacgccatca agatgggcac gaccgtcgcg accaacgcgc
     2761 tgctcgagcg caagggcacg cccaccgtgc tggccatcac caggggcctc ggcgactgcc
     2821 tcaagatcgg ctaccagcac cgcccggaca tcttcgcccg ccacatcgtc ctgcccgagc
     2881 agctctatag ccgcgtcgtc gagatcgacg agcggctgct ggccgatggt gcggtcgacc
     2941 gcccgctcga cgaggcccgc agccgggccg acctccaggc cgccttcgac gagggctttc
     3001 gggccgtcgc catcgtcctg atgcacggct accgccatcc ggatcacgag agccgcgtgg
     3061 ccgagctcgc ggccgggatc ggcttcaccc ggatctcggt gagccatcag gtctcgccgc
     3121 tcatgaagat cgtcggccgc ggcgacacga ccgtggtcga cgcctatctc tcgcccattc
     3181 tgcgccgcta tgtcgaccgc gtcgccgccc ggacggaggg ggtgcggctg atgttcatgc
     3241 agtcgaatgg cggcctcgtc gatgcccaca ggttccaggg caaggacgcc atcctgtcgg
     3301 gtccggccgg cggcatcgtc ggcgccgcgc gcaccgccgc catcgccggg ttcgagcgga
     3361 tcatcggctt cgacatgggc ggcacctcca ccgacgtcac ccactatgcc ggcgagtacg
     3421 agcgcgcctt cgagaccgag gtggcaggtg tgcggatgcg ggcgccgatg atgaagatcc
     3481 acaccgtcgc cgcgggcggc gggtcgatca tcaccttcga cggccagcgc tttcgggtcg
     3541 gcccggagag cgccggcgcc gaccccggcc ccgcctgcta ccgcaagggc gggcccttgg
     3601 ccgtcaccga cgccaacctc atggtcggca agctgctccc cgacttcttc ccgcacgtct
     3661 tcggccccgc cgccgaccag cccctcgacg aggcggtcgt gcgcgagcgg ttcgcggcgc
     3721 tcgcggcaga ggcgagcgtc gccaccggcg tcgagcgccg cccggaggag atcgcccagg
     3781 gctgcctcgc catcgccaac gacaacatgg cgaacgccat caaggagatc tcgatcgccc
     3841 gcggcatcga cgtgcagggc tacaccctcg tctgcttcgg cggcgccggc ggccagcatg
     3901 cctgccaggt ggcggacctg ctcggcatcg gcagcgtcca gatccacccc tatgccgccg
     3961 tgctctcggc ctacggcatg ggcctcgccg acctgcgcac catccgccag cgtgccctcg
     4021 acacggcgct ggagcccggc atcgaaggcg agctcgaggc ggcccgcgcc gagctcgcct
     4081 gctcggccgc cgccgagctc gccgagcagg ggatcgagcc cgaggcgctt cggctcgagg
     4141 cccgcgccca tatccgctat gccggcaccg acacgccgat cgaggtgccg ctcggcacgc
     4201 cggatgccat gcgcgccgcc ttcgaggcgg agcacaagtc ccgctacggc ttcaccgtcg
     4261 agggccgcgg cctcgtggtc gagacgctga ccgtcgaggg catcggcctg atgcaggcga
     4321 tcgacgagcc cgaggtgcag gtggcagccg accgcgaccc gccggcgccg gtcggcaaag
     4381 cccatatgtt caccacgagc gcaccccatc agccggccga gaccttcgag gccgccgtct
     4441 ggcggcgcga ggacctggcg cggggcgatc gggtgcgtgg cccggccatc gtcgccggca
     4501 gcaccaccac catcgtcgtc gagccgggct gggcgctcga ggtgacaccg cgcgaccacg
     4561 tcgtgctgac ccgcgtcgag aagctgcccg agcgcgtcgc cctcggcacg cattgcgacc
     4621 cgatcctgct cgagatcttc aacaatctct tcatgaccat tgccgagcag atgggggtga
     4681 cgctgcgcaa caccgcctac tcggtcaacg tcaaggagcg gctggacttc tcctgcgccg
     4741 tcttcgacga gacgggcggc ctgatcgcca acgccccgca catgcccgtg cacctgggct
     4801 cgatgggcga atcggtccgc acggtgatcc ggctgcggcg ggacaccatg ggcccgggcg
     4861 acgtctacgc cctgaacaac ccctataacg gcggcacgca ccttcccgac gtcaccgtcg
     4921 tcatgccggt cttcggcgag gcgggggagg tgctcttcta caccgcgtcg cgcgcgcacc
     4981 atgccgatat cggcggcatc acgcccggct ccatgccgcc cttcagccgg cacatcgacg
     5041 aggagggcgt gctcttcgac gacttccacc tggtcgaggc cggccgcttc cgccacgacg
     5101 ctgtcgaagc gctcctgaag tccggcccgc acccggcccg caaccccagg cagaacatcg
     5161 ccgatctcca agcccaggtg gcgtcctgcg ccaagggggc ggccgagctc gaccgcatgg
     5221 tcggccagtt cggcatcgag acggtccggg cctacatgcg ccacgtcatg gacaatgccg
     5281 aggagagcgt gcggcgcgtc ctcgaccggc tccaggacgg ggccttcacc tatgcccacg
     5341 accgggggcc ggtcatcgag gtgaaggtca cggtcgaccg ggccggccgc tcggccagga
     5401 tcgacttcac cggcacgagc ccgcagcagg actggaactt caacgccccg gccgccgtca
     5461 cccgggccgc cgtgctctac gtcttcagga cgctggtcga cagcgacatc ccgctcaacg
     5521 acggctgcct gaagccgctc gagctcgtca tccccgaggg ctcgatcctc gcctgcaagc
     5581 atccggccgc cgtctgcgcc ggcaatgtcg agacctcgca gtggtgcacc gacacgctct
     5641 tcggcgccct gggagccctg gccgcggccc aggggacgat gaacaacttc accttcggca
     5701 acgccactca ccagtactac gagaccatct gcggcggctc cggcgccggc cccgaccatc
     5761 cgggcacatc cgccgtccac acccacatga ccaactcgcg cctgaccgac cccgagatcc
     5821 tcgaatggcg ctatcccgtc ctcctcgagg agcaccgcat ccgcagaggc tccggtggca
     5881 aggggcgctt tgccggcggc gacggcacgc tgcgccgcat ccgcttcctc gagcccatgg
     5941 ccgcggcgct cctcgccaac caccgcaagg tgcccccctt cggcctcgac ggcggcgagc
     6001 ccggccaatg cggcgaccag tgggtcgagc gccgcaacgg caaggtggag aagctcgagg
     6061 gctgcgacgc gacggagctg gaggccggcg acgtgttcgc catccagaca ccgacgggtg
     6121 gggggtatgg caaggcgtag ccgacgaccg cgcggcctcg cgagcaacgc ggcc
//
LOCUS       DAOTFI010000268         6144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_15168_length_6144_cov_5.891279, whole genome shotgun sequence.
ACCESSION   DAOTFI010000268 DAOTFI010000000
VERSION     DAOTFI010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15168_length_6144_cov_5.891279"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="P5558_20620"
     CDS             <1..910
                     /locus_tag="P5558_20620"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY26785.1"
                     /translation="WQDWQRRLDAPEGPITPCPPPEPRPPAAARPRELWVTDIERLMR
                     DPYSIYARRILGLKELDPLDADPGASDYGQLIHEVLQRFVQLHPQALPADAEEEIVRI
                     GLEAFARLASRPEVRTLWWPRFRRVAGWFIEQERARRGRIERILVETRGSLIVDEAGR
                     YTIRARADRLEIGPDGAIGIGDYKTGSIPRPPDIARGLNPQLPLEAAIARGGGFEGAS
                     SQEVAEIAYWPVKASLVPPRIEVRGSGEDLGLQIEAALDGVRRLFAVFDDSQTPYFSV
                     PRPEIAPAFNPYEHLARIKEWRGGGD"
     gene            903..4328
                     /gene="addA"
                     /locus_tag="P5558_20625"
     CDS             903..4328
                     /gene="addA"
                     /locus_tag="P5558_20625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HRY26786.1"
                     /translation="MTESPAQVPATQQRPTVQQSRAADPGHSVWVTANAGTGKTRVLT
                     SRVLRLLLAGNDPESILCLTFTRAAAAEMAARIEAQLAAWAIQKDSEKLAGDLETLLG
                     EPADGSTIDRARRLFAQVLDLPSGLSITTIHAFCQSLLRRFPLEAGIAPHFELIDERT
                     ARERQIEARQQVLGSGDASLRQAIEEVAILLADGSLTEALGQLLQMRDHLLALADRHG
                     GPAGVIDRICERLEVDPRDTPEAVVARACRPEEVDSHRLVPAANALIGGAATHDARGH
                     TTLAWLNLSEKDRNRTFEDYSRHYLKKDGEPLARLGPNTLDPAHYRALLAEQARLVRV
                     GQRLKALEVARRTRALLALGYAVIEAYAAAKRQAAALDYDDLIQRTAALLADPGRREW
                     VRFKLDARLDHLLVDEAQDTSPAQWSIIEHLVEEFHAGEGSGRVDRTLFVVGDEKQSI
                     FSFQGADIASFAALRERLSTRFRQAGRPLDRSVLDLSFRSGPAIVELVEAMLADPVVR
                     SGVLADPGAAVQHRTFRQNALSSVEIWPLERPVARPQPAVEGWVLPTERHDTDEPETR
                     LAQRIARTIGDWLDAGIVPESTGRKLRPGDILILLQRRGVAQERILKALKERGIPVAG
                     ADRLKLTEAIAVEDLVALGRALLLPEDDLNLAALLKSPLVGLGEDDLFRLAHDRDRRP
                     LVERLRHLGEAGEAPFAAAWERFSGWLARADFMPPFELYARILGPEGGRRRLLQRLGP
                     DAAEPIEAFLAQALAHEAGHPASLQGFIHWLGLESGQLKRDLEQRADAVRVMTVHGSK
                     GLEAPFVLLADAGPYAPPAGRDRLIVDPDTGLPIWRAAQAQRDPVSEAITTERQRLAE
                     AERWRLFYVAITRAQDRLVVAGWDRQRECEAPSWHAVLSQALQAMPDVETREQGELHL
                     VRGVAGAGPAEPAAPAVAPAPLPDWLDRPPPPEPQRRRLVHPSAEEVLAMPASSPLAE
                     QGRGARVFGTALHRLLHEVAALPDDDRRDFLDLRLATWPGLEPDARAELARQVLGVLE
                     LPGLAPAFAAGSRSEQPVIGRVGDLVIAGQIDRFAVTSEAVFLVDFKSNRLPPATVDQ
                     VPVAYLRQLAAYAVLLGRLYPGRAVKAALVWTAVPALMPVPTALLAIHAPEPA"
     gene            4410..4730
                     /gene="trxA"
                     /locus_tag="P5558_20630"
     CDS             4410..4730
                     /gene="trxA"
                     /locus_tag="P5558_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HRY26787.1"
                     /translation="MSVHQTNDADFSTDVLKSATPVVVDFWAEWCGPCKMIAPHLEEM
                     ANEMDGKVKVVKVNIDENPLTPTKYGVRGIPTLMLFKDGEVAATKVGAAPKTKLVEWV
                     EESL"
     gene            4751..6136
                     /locus_tag="P5558_20635"
     CDS             4751..6136
                     /locus_tag="P5558_20635"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRY26788.1"
                     /translation="MGGTGTERDDAASQDRLAVFLADNPLVESVDVILPDIVGIARGK
                     RFDIGELRAALRGEALFSSTLYALDTTGANVDRSGLVWEEGDADRPIRLDDATLAPVP
                     WASERAQILGGLGEADGQPFFADPRAVLGRVAEGFAAFGLKPVCALELEFYLLDLALD
                     ADGLGQLPTSTRLGRRPREVEVYSFDRLREIEPFLDLVADYCDAQDIPAKGAVAEFAP
                     GQFEVNLRHVHDPVRAADHALLFKRCVKAAALESGFRACFMAKPFPETSGSGLHIHLS
                     LEDEDGRNVLADGEAGELTLRHAVWGLQQAMAESMLVFAPNANSYRRLQPLSYAPTAP
                     SWGINNRTVALRVPAGPAAARRIEHRAPGADANPYLATAAMLAGVLHGLEHKGDPGPP
                     MTGNAYEKARRSVPTNWAEALLAFERGSLLKRFLGERFCALYAACRRAEFDRFEARLT
                     PLDFEWYLSAV"
BASE COUNT          872 a         2201 c         2199 g          872 t
ORIGIN      
        1 ctggcaggac tggcagcggc ggctggatgc gccggagggg ccgatcacgc cctgcccgcc
       61 tcccgagccg cggccacccg ccgccgcgcg gccgcgcgag ctctgggtca ccgacatcga
      121 gcggctgatg cgcgatccct acagcatcta cgcgcggcgc attctgggac tgaaggagct
      181 cgacccgctg gatgccgacc cgggtgccag cgactacggc cagctcatcc acgaggtgct
      241 gcagcgcttc gtccagctcc atccgcaagc gctccccgcc gatgccgagg aggagatcgt
      301 caggatcggg ctcgaggcgt tcgcgcggct cgcctcgcgg cccgaggtcc ggaccctctg
      361 gtggccgcgc tttcgccggg tcgccggctg gttcatcgag caggaacggg cgcggcgcgg
      421 gcgcatcgag cgcatcctcg tcgagacgcg cggcagcctc atcgtcgacg aggcgggacg
      481 ctacaccatc cgggcgcgtg ccgaccggct cgagatcggc ccggacggtg cgatcggcat
      541 cggcgactac aagacgggca gcattccgcg cccgccggac atcgcccggg gcctgaaccc
      601 gcaattgccg ctcgaggcgg cgatcgcgcg gggcggcggc ttcgagggcg ccagcagcca
      661 ggaggtcgcc gagatcgcct attggccggt caaggcgagc ctcgtgccgc ccaggatcga
      721 ggtgcggggc tcgggcgagg acctgggcct gcagatcgag gcggcgctcg acggcgtgcg
      781 gcggctgttc gcggtcttcg acgattcgca gacgccctat ttctcggtgc cgcggccgga
      841 gatcgcaccg gccttcaacc cgtacgagca tctggcgcgg atcaaggaat ggcggggtgg
      901 cggtgactga gagcccggcc caggtccccg ccacgcaaca gcgcccgacc gtgcagcaga
      961 gccgggcggc cgatcccggg cactcggtct gggtcacggc caatgccggc accggcaaga
     1021 cccgggtgct gaccagccgg gtgctgcgcc tgctgctcgc cggcaacgac ccggagtcga
     1081 tcctctgcct caccttcacc cgcgccgccg cggccgagat ggcggcccgg atcgaggcgc
     1141 agctcgcagc ctgggcgatc cagaaggaca gcgagaagct cgcgggggac ctcgagaccc
     1201 tgctgggcga gcctgcggac ggctccacca tcgaccgggc gcgccggctc ttcgcccagg
     1261 tgctcgacct gccgagcggg ctgtcgatca cgacgatcca tgcgttctgc cagtcgctgc
     1321 tccgccgctt tccgctggag gcgggcattg ccccgcattt cgagctgatc gacgagcgga
     1381 cggcgcggga aaggcagatc gaggcccggc agcaggtgct gggaagcggg gatgcgagcc
     1441 tgcggcaggc catcgaggag gtggcgatcc tgctcgccga cggctcgctg accgaggcgc
     1501 tcgggcagct gctccagatg cgcgaccatc ttctggccct tgccgaccgg catggcgggc
     1561 ctgccggcgt gatcgacagg atctgcgaga ggctcgaggt cgatccgcgc gatacgccgg
     1621 aggcggtagt tgcccgggcc tgccggccgg aggaggtcga cagccatcgc ctggtgccgg
     1681 ctgccaacgc gctgatcggt ggtgccgcta cccacgacgc acgcggccac acgacccttg
     1741 cctggctgaa tttgtcagag aaggaccgca atcgcacatt cgaggactat tcacggcatt
     1801 acctcaagaa ggacggcgag ccgctcgccc ggctgggacc gaacacgctt gatccggcgc
     1861 actatcgggc gctcctcgcc gagcaggcgc ggctggtgcg ggtcgggcag cggctcaagg
     1921 cgctcgaggt ggcgcggcgg acgcgggcgc tcctggcctt gggctatgcg gtgatcgagg
     1981 cctacgccgc ggccaagcgg caggcggcgg cgctcgacta tgacgacctg atccagcgca
     2041 cggcagccct cctcgccgat cccggccggc gggaatgggt gcggttcaag ctcgatgcgc
     2101 gtctcgatca cctgctggtc gacgaggccc aggacacgag ccccgcgcag tggtcgatca
     2161 tcgagcatct ggtcgaggag ttccatgccg gcgagggctc ggggcgggtc gaccgcacgc
     2221 tcttcgtcgt cggcgacgag aagcagtcga tcttcagctt ccagggggcc gacatcgcca
     2281 gcttcgccgc cttgcgcgag cggctctcga cgagattccg gcaggccggg cggccgctcg
     2341 accggagcgt gctcgacctc tccttccgct ccggccccgc catcgtcgag ctggtcgagg
     2401 ccatgctcgc cgatccggtc gtgcgttccg gcgtgctcgc cgacccgggg gcggcggtgc
     2461 agcaccggac cttccgccag aatgccctga gctcggtcga gatctggccg ctcgagcggc
     2521 cggtcgcgcg gccacagcct gcggtagagg gttgggtcct gccgaccgag cgccatgaca
     2581 cggacgagcc ggagacgcgt ctcgcccagc gaatcgcccg gaccatcggg gactggctgg
     2641 acgccggcat cgtcccggaa tcgaccggcc gaaagctgcg gccgggcgac atcctgatcc
     2701 tcctgcagcg ccgcggtgtg gcccaggagc ggatcctcaa ggcgctgaag gagcggggca
     2761 ttcccgtcgc cggcgccgat cggctgaagc tcaccgaggc catcgccgtc gaggacctgg
     2821 tggccctcgg ccgggcactg ctgctgccgg aggacgatct caacctcgca gccctgctga
     2881 agagcccgct ggtcggcctg ggcgaggacg atctcttccg cctcgcccac gatcgtgacc
     2941 gccgcccgct cgtcgagcgc ctgcggcatc tcggcgaggc cggcgaggca ccctttgccg
     3001 ccgcctggga gcgcttctcc ggctggctcg cccgggccga cttcatgccg cccttcgagc
     3061 tctacgcccg catcctcggg ccggagggcg ggcggcggcg gctgctgcag cggctcgggc
     3121 cggatgccgc cgagccgatc gaggcgtttc tcgcccaggc cctcgcccat gaggccggcc
     3181 atccggcgtc gctgcagggc ttcatccact ggctcgggct ggagagcggc cagctcaagc
     3241 gcgacctcga gcagcgcgcc gacgcggtgc gggtgatgac ggtgcacggc tcgaagggtc
     3301 tggaggcgcc gttcgtcctg ctcgccgatg ccggccccta tgcgccaccg gccgggcgcg
     3361 accggctgat cgtcgacccg gatacgggcc tgcccatctg gcgcgcggcc caggcacagc
     3421 gcgatccggt gagcgaggcc atcacgaccg aacgacaaag gctcgccgag gccgagcgct
     3481 ggcggctgtt ctatgtggcg atcacccgcg cccaggaccg gctggtcgtc gccggctggg
     3541 acaggcagcg cgagtgcgag gcgccatcct ggcacgcggt gctgagccag gcgctgcagg
     3601 ctatgccgga cgtcgagacc agggagcagg gcgagctgca tctggtgcgc ggcgtggccg
     3661 gcgcggggcc ggccgagccc gcggcgccag cggtcgcgcc ggccccgctc cccgactggc
     3721 tcgaccggcc gccaccgccc gagccacagc ggcgccgcct cgtgcatcca tcggcggagg
     3781 aggtcctggc gatgccggcg agctcgcccc tggccgaaca aggcaggggg gccagggtct
     3841 tcggcacggc gctacaccgc ctgctgcacg aggtcgcggc cctgcccgat gacgatcgga
     3901 gggactttct cgacctgcgc ctcgccacct ggccggggct ggagccggat gcccgggccg
     3961 agctggcgcg tcaggtgctg ggcgtgctcg agctccccgg gctggcgccg gccttcgcgg
     4021 ccggaagccg ctccgagcag ccggtcatcg gccgcgtcgg cgacctcgtg atcgccggac
     4081 agatcgaccg cttcgccgtg acctcggagg cggtcttcct cgtcgatttc aagtccaacc
     4141 gcctgccgcc ggcaacggtg gaccaggtgc cggtggccta tctgcgccag ctcgcggcct
     4201 atgccgtcct gctcggccgg ctctatcccg gccgggcggt gaaggccgcg ctcgtctgga
     4261 ccgcggtgcc ggccctgatg ccggtgccca cggcgctgct tgccatccat gcgcccgagc
     4321 cggcttgacg gcatgcggca cctgatccaa gactccgctc ccgcagcagt gcccgccggg
     4381 caccgacaca cagcgacgga tacgcgccga tgagtgtgca ccagacgaac gatgccgact
     4441 tcagcaccga cgtcctcaag tcggcgaccc ctgtcgtcgt cgatttctgg gccgaatggt
     4501 gcggcccctg caagatgatc gcccctcatc tcgaggagat ggcgaacgag atggacggca
     4561 aggtcaaggt ggtgaaggtc aatatcgacg aaaaccccct cacccccacc aagtacggtg
     4621 tccggggcat cccgacgctg atgctcttca aggacggcga ggtggcggcg accaaggtcg
     4681 gtgccgcccc caagacgaag ctggtcgagt gggtcgagga gagcctctga ccaccacgcc
     4741 atcctcggcc gtgggcggaa ccgggacaga acgcgacgac gcggcgagcc aggatcggct
     4801 cgccgttttt ctcgccgaca accccctggt cgagagcgtc gacgtgatcc tgccggacat
     4861 tgtcggcatc gcccgcggca agcgcttcga catcggcgag cttcgggcgg cgctgcgcgg
     4921 cgaggcgctc ttctcgagca cgctctacgc gctggatacg accggtgcca atgtcgaccg
     4981 cagcggcctc gtctgggagg agggcgatgc cgaccggcct atccgcctcg acgacgcgac
     5041 cctcgcaccg gtgccctggg cgtccgagcg tgcgcagatc ctgggcggcc tcggcgaggc
     5101 ggacggccag cccttcttcg cggatccgcg ggccgtgctc ggccgggtgg ccgagggctt
     5161 cgcggcgttc ggcctgaagc cggtctgcgc cctcgagctg gagttctacc tgctcgacct
     5221 agcgctcgac gcggacgggc tcggccagtt gccgacgagc acgaggctcg gccgccggcc
     5281 gcgcgaggtg gaggtctact ccttcgaccg cctgcgcgag atcgagccct tcctcgacct
     5341 cgtcgccgac tactgcgacg cgcaggacat cccggccaag ggggcggtcg ccgagttcgc
     5401 gccggggcag ttcgaggtca acctgcgcca tgtgcacgat ccggtgcgcg ccgccgacca
     5461 tgcgctgctg ttcaagcgct gcgtcaaggc ggcagcgctg gagagcgggt ttcgcgcctg
     5521 cttcatggcc aagcccttcc ccgagacgag cggcagcggg ctgcacatcc atctcagcct
     5581 cgaggacgag gacggccgga acgtgctggc ggacggcgag gcgggcgagc tgacgctgcg
     5641 ccatgccgtc tggggcctgc agcaggcgat ggcggaatcg atgctcgtct tcgcccccaa
     5701 cgccaacagc taccggcggc tgcaaccgct ctcctacgca cccaccgcac cgagctgggg
     5761 gatcaacaac cgcaccgtgg cgctgcgcgt gccggcgggg cccgcggcgg cgcggcggat
     5821 cgagcaccgt gcccccgggg cggatgccaa tccctatctc gccaccgccg ccatgctcgc
     5881 cggcgtgctg cacggtctcg agcacaaggg cgatccgggg ccgcccatga ccggcaatgc
     5941 ctacgagaag gcacgtcgaa gcgtgccgac caactgggcg gaagcacttc tggccttcga
     6001 gcgcggcagc ctgctcaagc ggtttctcgg cgagcggttc tgcgcgctct atgccgcctg
     6061 ccggcgggcc gagttcgacc gcttcgaggc gcggctcacc ccgctcgact tcgagtggta
     6121 tctctcggcc gtctgagcgc tcag
//
LOCUS       DAOTFI010000269         6099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_15319_length_6099_cov_5.447386, whole genome shotgun sequence.
ACCESSION   DAOTFI010000269 DAOTFI010000000
VERSION     DAOTFI010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15319_length_6099_cov_5.447386"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="P5558_20640"
     CDS             complement(<1..706)
                     /locus_tag="P5558_20640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26789.1"
                     /translation="MVLLPPTLLERVGGAARRLPRDLLASLLVVLAYATFTIMAVFVR
                     NAAEHLPVIVIVFLRQVLTMLLMTPLFWMNREQIRHPMGFRLHALRGFTAVGSMLCGL
                     TALVYVPFADVTAIQMTEALFVTALAALLLRETVGWRRWSATAVGFLGVVVMLGPFSG
                     GIEHYALVALLGAVFSAFAVISLRLGSTLDSTVTVLFYQGLVVLALTAPLAWWYWQPV
                     TLAGLQVVFMMSLVMVV"
     gene            complement(807..1847)
                     /locus_tag="P5558_20645"
     CDS             complement(807..1847)
                     /locus_tag="P5558_20645"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY26790.1"
                     /translation="MAGDNGRDGEGWATMGEVAARAGVSPMTVSRVLKEPGRVRPTTR
                     ARVEAAIAELGYVPDAAAGALASRQSRLVAALVSTLAGSIFASTIAGLGAVLREAGHE
                     LLLGTTEYSSSSEEALLRAVLGRRPGGLVLSAGQHTPAARRLLERATRGLGVPVVEIW
                     QLPERPVDMAVGFSNFAAGRAMTAFLADLGYRRIAFVGVLGPDDFRGLRRHAGYREAL
                     AERGLGEPRAFGSGGSGGSVEDGARGLAAVLERWPDCDAVFCGSDPLAFGAIAEAARR
                     GLAVPGELAVAGFGDFDYAGEAGLALTTVRIPGERIGRETARLLLERKAGRLEGPVVV
                     DLGFELVRRRTA"
     gene            1915..2820
                     /locus_tag="P5558_20650"
     CDS             1915..2820
                     /locus_tag="P5558_20650"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY26791.1"
                     /translation="MQGPGLSEGDLAFYRENGYLMIEDAVSPEDLAELQAVARDFIDR
                     SRRVAESNDIYDLDEGHSPSNPRLTRIKLPHKQHPVFDRVLRSDRMKSYFTALLGPDV
                     VLQTSKLNTKAPGGGAAVEWHQDWAFYPHTNDDMLAFGLMLEDVDEANGPLMAIPGSH
                     KGPLLSHHNNGVFAGAVSPDDPDFHFDQAVTLTGKAGSMTVHHARTLHGSAPNRSDRA
                     RLILFYEACAADAWPLLGAGSYIHRLPQREMWADLLERMVCGEPVLEPRIEKVPVRLP
                     LPPAPDATSIFKTQKSGGARSAFAA"
     gene            2874..3857
                     /locus_tag="P5558_20655"
     CDS             2874..3857
                     /locus_tag="P5558_20655"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY26792.1"
                     /translation="MLETLKGALAGAALLALAAAPATAATTLTFGGSDAVGTILDRGN
                     AMFAELVTERTNGEVTVNFISGEQLGTDIDVIQAMMQGSVALYGDVVEWYGNWVNDFN
                     IMSWGFTFDDNDHMQAFLDSDIYAALAEKLREEQGLRILAAAPTQPRILFATRPVATP
                     DDLAGLKMRVPEIRAYLLLWQTLGTEPSRLAWGEIYLGLSTGTVEAAEGPVASAYAAK
                     LHQVATNVMRTDHLVSTYHVTINEDVFQSLTPEQQQIMIDTAREVTQWGRAEAERQTE
                     ETVAQMAAEGATIHEVDVAPFAEKARAGVEEMEKDGVWSPGLWQKIRDMAP"
     gene            3894..4472
                     /locus_tag="P5558_20660"
     CDS             3894..4472
                     /locus_tag="P5558_20660"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY26793.1"
                     /translation="MIEPCRRLLGLLGRLEQALVVLLLVNIVLNILAQVISRYFFGKP
                     LVWVEEIATYSFIWATFLGAALALKYDRHVKIDTFVARLSERQGAVARAVVFLVIMAL
                     LATILPSLVSAIEVEMRRSSIALPVQIPSGWFFSVPLAVGVVSMLLTAVFKVLVELRF
                     ALGGEPPPLIMRPLPDDHDDDAVEQAMLGDRL"
     gene            4469..5752
                     /locus_tag="P5558_20665"
     CDS             4469..5752
                     /locus_tag="P5558_20665"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY26794.1"
                     /translation="MNVWIMFLAFAVLVAIEVPIAFALAASAVVWLTLESPIPLTIVV
                     QRMASGLDSFPLLAIPLFILAGSLMNRAGLAARIFAFALSLFGHVRGSLAHVNVAASI
                     VFAGMSGVAQADAAGLGTVEIRAMRKAGFDPAFSAAVTAASSIIGPIIPPSVIMVIYA
                     VLAQVSVADLFLAGILPGFLMGALLMALIYWLARTGRITAPTLPRASIGEVGTTFVRA
                     LPALLAPVILVAGLLSGAATPTELGAVTVAYAMVVGFAQRELTFRKLHLCLVETGITT
                     GVLVFIIAAAVPFGWVVSVNNLPAALTSTLLGISDNPYVILFLVNLLLLAAGLVMETT
                     AILLIAVPALLPLALALKIDLVHFGIIMVLNLLIGATTPPFGVILFIMMDIARISFAQ
                     MVRAMLPFYIPLVAALLVVTFWPDLVLFVPRLIGG"
     gene            complement(5792..>6099)
                     /locus_tag="P5558_20670"
     CDS             complement(5792..>6099)
                     /locus_tag="P5558_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26795.1"
                     /translation="HFELWGGDDPMAALFLTANAAVFAPDHARPIYERTWVYRGDDHG
                     YFEMAAGGATLLRRELAMAVDRLSAKIVSDLMIGAGAEVHPTDGQPAGTVWTVSPES"
BASE COUNT          955 a         2342 c         1919 g          883 t
ORIGIN      
        1 cgaccaccat gaccagcgac atcatgaaaa ccacctgcag gccggccagg gtcaccggct
       61 gccagtacca ccaggcgagc ggtgcggtca gggcgagcac cacgagaccc tgatagaaga
      121 gcacggtgac ggtcgaatcg agcgtcgagc cgagccgcag cgagatcacg gcgaaagcgc
      181 tgaacacggc acccagcagc gccaccaggg cataatgctc gatcccgccc gagaacgggc
      241 cgagcatgac caccacgccg agaaagccca ccgccgtcgc cgaccagcgt cgccagccga
      301 ccgtctcgcg cagcagcagg gccgccagcg cggtgacgaa cagcgcctcg gtcatctgga
      361 tcgcggtgac atcggcgaac ggcacgtaga cgagggcggt gagcccgcag agcatggagc
      421 cgacggcggt gaagccgcgc agcgcatgca gccgaaaccc catgggatgg cggatctgct
      481 cccggttcat ccagaagagc ggcgtcatca gcagcatggt cagcacctgc cgcaggaaca
      541 cgatgacgat gaccggcagg tgctcggcgg cgttgcgcac gaagaccgcc atgatggtga
      601 aggtcgcata ggccagcacg accaggagcg atgccaggag gtcgcgcggc agccggcggg
      661 ccgccccgcc gacccgctcg agcagcgtgg gcggcaacag aaccaagtcg gcaccaaaaa
      721 gacgggcaga gccggtaagc aaccaagagt ttagaagggt aacggccgcg ccgccagtcc
      781 tcctttcgcg tcagtcaggc ggtcgttcag gcggtccggc ggcggaccag ctcgaagccg
      841 agatcgacca cgaccggccc ctcgagccgg ccggccttgc gctcgagcag cagccgggcg
      901 gtctcgcggc cgatccgctc gcccgggatc cgcaccgtgg tcagcgccag cccggcctcg
      961 cccgcatagt cgaaatcgcc gaacccggcg acggcgagct cgcccggcac cgcgaggccg
     1021 cgcctggccg cctcggcgat ggcgccgaag gcgagcggat cgctgccgca gaagacggca
     1081 tcgcaatccg gccagcgctc gagcacggcg gcgagacccc gcgcaccatc ctcgaccgag
     1141 ccacccgacc cgccggagcc gaaggcccga ggctccccga gcccgcgttc cgccagcgcc
     1201 tcccggtagc ccgcatggcg ccgcagcccg cggaaatcgt ccggcccgag gacgccgaca
     1261 aaggcgatcc gccgatagcc gagatcggcg aggaacgccg tcatcgcccg cccggcggcg
     1321 aagttggaga agcccaccgc catgtcgacc ggccgctccg gaagctgcca gatctcgacc
     1381 accggcacgc ccaggccgcg cgtcgcccgc tcgagcaggc ggcgtgccgc cggcgtgtgc
     1441 tgcccggcgc tcagcaccag cccgcccggc cgccggccga gcaccgcgcg caggagcgcc
     1501 tcctcgctcg aagaggaata ctcggtggtg cccagcagga gctcgtggcc cgcctcgcgc
     1561 agcacggcgc cgagcccggc gatggtggag gcgaagatcg agccggcgag ggtcgagacg
     1621 agcgccgcca ccagccggct ctgccgcgac gcgagcgcgc cggccgccgc gtccggcaca
     1681 tagccgagct cggcgatcgc cgcctccacc ctggcccggg tcgtcggccg cacgcggccc
     1741 ggctccttga gcacgcggct gaccgtcatg ggcgagacgc cggcccgggc cgccacctcg
     1801 cccatcgtgg cccagccctc gccgtccctg ccattgtcac cggccacgcg atccccttga
     1861 cgctagagat gttagcgcta ccataatgtc gtcaatccac ggagggaagt gaccatgcaa
     1921 ggcccgggcc tgagcgaggg cgacctcgcc ttctatcgcg agaacggcta cctgatgatc
     1981 gaggacgccg tctcgcccga ggatctggcc gagctgcagg cggtcgcccg cgacttcatc
     2041 gaccgctcgc gccgcgtcgc cgagagcaac gacatctacg acctcgacga gggccattcg
     2101 ccgtccaacc cgcgcctgac gcggatcaag ctgccgcaca agcagcaccc cgtcttcgac
     2161 cgcgtgctgc gctcggaccg catgaagagc tacttcacgg cgctcctcgg ccccgacgtc
     2221 gtcctgcaga cctcgaagct caacaccaag gcgccgggcg gcggggcggc cgtggaatgg
     2281 caccaggact gggccttcta cccgcacacc aacgacgaca tgctggcctt cggcctgatg
     2341 ctcgaggacg tcgacgaggc caacggcccg ctcatggcga tccccggctc gcacaagggg
     2401 ccgctcctga gccaccacaa caacggcgtc ttcgccggtg cggtgagccc ggacgacccg
     2461 gacttccact tcgaccaggc ggtcacgctc accggcaagg ccggctcgat gaccgtccac
     2521 cacgcccgca cgctgcacgg ctcggccccc aaccgcagcg accgggcgcg cctcatcctc
     2581 ttctacgagg cctgcgccgc cgacgcctgg cccttgctcg gcgccggctc ctacatccac
     2641 cgcctgccgc agcgcgagat gtgggccgac ctcctggagc gcatggtctg cggcgagccg
     2701 gtgctcgagc cgcgcatcga gaaggtgccg gtgcgcctgc cgctgccgcc ggcccccgac
     2761 gccacctcga tcttcaagac ccagaaatcc ggcggggcgc ggagcgcttt cgccgcctga
     2821 gcttcaggac tacgctggtc gacgaaaaaa caacatggag ggaggcagga accatgctcg
     2881 agacgttgaa gggcgccctc gcgggcgccg cccttttggc gctcgccgcg gcaccggcca
     2941 cggccgccac caccctcacc ttcggcggct cggacgccgt cggcaccatc ctcgaccgcg
     3001 gcaacgccat gttcgcggag ctggtgaccg agcgcaccaa tggcgaggtc acggtcaact
     3061 tcatctccgg cgagcagctc ggcaccgaca tcgacgtcat ccaggcgatg atgcagggct
     3121 cggtggcgct ctatggcgac gtggtcgagt ggtacggcaa ctgggtgaac gacttcaaca
     3181 tcatgagctg gggcttcacc ttcgacgaca acgaccacat gcaggccttc ctcgacagcg
     3241 acatctacgc ggcgctcgcc gagaagctgc gcgaggagca ggggctccgc atcctcgccg
     3301 ccgccccgac ccagccccgc atcctcttcg ccacccgtcc ggtggcgacg ccggacgacc
     3361 tcgccggcct caagatgcgc gtgccggaga tccgggccta cctcctgctc tggcagacgc
     3421 tgggcaccga gccctcgcgc ctcgcctggg gcgagatcta tctcggcctc tcgaccggca
     3481 cggtcgaggc ggcggaaggc ccggtcgcct cggcctatgc cgccaagctg caccaggtgg
     3541 ccaccaacgt catgcgcacc gaccacctcg tctcgaccta ccacgtcacc atcaacgagg
     3601 acgtgttcca gagcctgacg ccggagcagc agcagatcat gatcgacacc gcccgggagg
     3661 tgacccagtg gggccgtgcc gaggccgagc gccagaccga ggagacggtg gcgcagatgg
     3721 ccgccgaggg ggccacgatc cacgaggtcg acgtcgcccc gttcgccgag aaggcgcgcg
     3781 ccggcgtcga ggagatggag aaggacggcg tctggtcgcc cggcctctgg cagaagatcc
     3841 gcgacatggc gccctgaggc cgggttcgcc cgactgacgc gagccttgcc tcgatgatcg
     3901 agccctgccg gcgcctgctc gggctgctcg gccggctcga gcaggcgctg gtcgtgctcc
     3961 tgctcgtcaa catcgtcctc aacatcctgg cccaggtgat ctcccgctac ttcttcggca
     4021 agccgctcgt ctgggtcgag gagatcgcca cctactcctt catctgggcc accttcctcg
     4081 gcgccgccct ggcgctcaag tacgaccggc acgtcaagat cgacacgttc gtcgcccgcc
     4141 tgtccgagcg gcagggtgcc gtcgcccggg ccgtcgtctt cctcgtcatc atggcactcc
     4201 tcgccaccat cctgccctcg ctcgtgagcg ccatcgaggt cgagatgcgc cgcagctcca
     4261 tagccctccc ggtgcagatc ccctcgggct ggttcttctc cgtgccgctc gccgtgggcg
     4321 tcgtctcgat gctgctgacc gccgtcttca aggtgctggt cgagctgcgc ttcgccctgg
     4381 gcggcgagcc gccgcctttg atcatgcggc ccctgcccga cgaccacgac gacgacgccg
     4441 tcgagcaggc gatgctgggc gaccgcctgt gaacgtctgg atcatgttcc tggccttcgc
     4501 cgtgctggtc gccatcgagg tgcccatcgc cttcgcgctg gctgccagcg ctgtcgtctg
     4561 gctcaccctc gagagcccga tcccgctcac catcgtcgtc cagcgcatgg cctcgggcct
     4621 cgacagcttc ccgctgctcg ccatcccgct cttcatcctg gccggctcgc tcatgaaccg
     4681 ggccggcctc gccgcccgca tcttcgcctt cgccctctcg ctcttcggcc acgtccgggg
     4741 cagcctcgcg catgtcaacg tggcggcctc catcgtcttc gccggcatgt ccggcgtcgc
     4801 ccaggccgac gccgccgggc tcggcacggt cgagatccgc gccatgcgca aggccggctt
     4861 cgacccggcc ttctcggccg cggtgaccgc cgcctcctcg atcatcggcc cgatcatccc
     4921 gccatccgtc atcatggtga tctacgcagt cctggcgcag gtctcggtcg ccgacctctt
     4981 cctcgccggt atcctcccgg gattcctgat gggcgcgctg ctcatggcgc tcatctactg
     5041 gctggcgcgc accggccgca tcaccgcccc cacgctgccc cgggccagca tcggcgaggt
     5101 gggcaccacc ttcgtccgcg ccctgccggc cctcctggcc cccgtcatcc tcgtggcggg
     5161 cctgctctcg ggcgccgcca cgcccaccga gctcggcgcc gtcaccgtcg cctatgcgat
     5221 ggtggtgggc ttcgcccagc gcgagctgac gttcaggaag ctccacctct gcctcgtcga
     5281 gacgggcatc accacgggcg tgctggtctt catcatcgcc gccgccgtgc ccttcggctg
     5341 ggtggtctcg gtcaacaacc tgccggccgc cctcacctcg accctgctcg gcatctccga
     5401 caacccctac gtcatcctct tcctcgtcaa cctgctcctg ctcgcggccg ggctggtcat
     5461 ggaaaccacg gccatcctcc tgatcgcggt cccggccctc ctgccgctcg ctttggcgct
     5521 gaagatcgac ctcgtgcatt tcggcatcat catggtgctg aacctgctca tcggcgccac
     5581 caccccgccc ttcggcgtca tcctcttcat catgatggac atcgccagga tcagcttcgc
     5641 ccagatggtc cgggccatgc tgcccttcta catcccgctc gtggccgccc tcctcgtcgt
     5701 caccttctgg cccgacctcg tgctcttcgt cccgcggctg atcggcggct gagtcgcagc
     5761 caacgcgtca cggcggtccc agagccggac atcagctctc tggactgaca gtccatacgg
     5821 ttccggctgg ctgcccgtcc gtgggatgca cttccgcacc ggcgccaatc atcagatcgg
     5881 acacgatctt cgccgaaagc cggtccaccg ccatggcgag ctccctgcga agcagggtcg
     5941 cgccgccggc tgccatctcg aaatagccgt ggtcgtcacc ccggtagacc caggtgcgct
     6001 cgtagatcgg ccgcgcgtgg tccggcgcga aaaccgcggc gttcgccgtc aggaacagcg
     6061 cggccatggg atcgtcaccg ccccacaact cgaaatgcg
//
LOCUS       DAOTFI010000270         6043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_15501_length_6043_cov_6.092685, whole genome shotgun sequence.
ACCESSION   DAOTFI010000270 DAOTFI010000000
VERSION     DAOTFI010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15501_length_6043_cov_6.092685"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="P5558_20675"
     CDS             complement(<1..1078)
                     /locus_tag="P5558_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRY26796.1"
                     /translation="MRAERRFVALLALVLGLAACTTSPPLRGTGDLGVVVERAEDSIL
                     IVDTTERQAIGRVAGLGDLSHASVVFSRDGRYAFVFGRDGGLSKVDLLTGEIAARVVQ
                     AGNSIGGAISQDGRIVAVANYEPGGVRFFRSDDLSPVAEVPSTYGADGARSKVVGIEE
                     IADNRFVFALYDADAIWTVGLEDGAAPEVERFAGVGKLPYDALLTPDGHHYIAGLFGE
                     DGLVVLDLWDEGAAPRRILDGYGKGEEPLPVYKMPHLEGWSVAGDRLYLPAIGRHELL
                     VVDRATFREIDRIPVHSQPVFAIARPDGRQVWVNFAHPDNDVIQVIDVPSGEIVEELR
                     PGPGVLHMEFTPKGEAVWISVRDAN"
     gene            complement(1068..1424)
                     /locus_tag="P5558_20680"
     CDS             complement(1068..1424)
                     /locus_tag="P5558_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY26797.1"
                     /translation="MPAASWVDRLALGLVIGGVAIAGPAGSAAAAEPSLQRQDELIHL
                     LRHDCGSCHGLTLKGGLGPPLLPDALADQPAESLVAIILDGVPGRPMPPWRGLLPEDE
                     VVWLVGRLQEGIGDAR"
     gene            complement(1402..2187)
                     /gene="cobA"
                     /locus_tag="P5558_20685"
     CDS             complement(1402..2187)
                     /gene="cobA"
                     /locus_tag="P5558_20685"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRY26798.1"
                     /translation="MPEKASVALVGAGPGDPELLTLKALRHFQTADVVVYDRLVSAAI
                     LALIPPGVQRICAGKAPGEHLLAQDAINALLVRLARAGRRVVRLKGGDPYVFGRGGEE
                     ALHLVAHGVPFEVVPGITAASGVLAAAGIPLTHRGMATGVRLITGHTCESDDLDIDWR
                     TLADPATTLVVYMALASLARFRDGLIGAGLAPSTPAVAIASGTQPGEQICRATLADLP
                     AAVEAAGLTAPVLLAVGSVVALGDILAAKSGLREEADACRQLG"
     gene            complement(2253..4019)
                     /locus_tag="P5558_20690"
     CDS             complement(2253..4019)
                     /locus_tag="P5558_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRY26799.1"
                     /translation="MTGRTALLAATALGAWALMAAPMGVAQEQPDPKALEGHTPPAGT
                     TYVPELNVLSEMKLAQPGLAPELQAMGVEPLSTDDFQRAQVIYFQRCAGCHGVLRKGA
                     TGRPLTPDIMKEKGADYVHAFIEYGSPGGMPNWGTSGDLTPEEVNIMARYLFHEPPQP
                     PEWGMETMRENWRVLVPVAERPTEKQNDLDLENLFSVTLRDSGEVALIDGATYEIKTI
                     LPTGYAVHISRLSASGRYLYVIGRDALVSMIDLWMDPPAVVAELKIGIEARSVESSKA
                     EGWEDKLVIAGAYWPPHYVIADGTTLEPLKVVSTRGYTVGEQEFHPEPRVASIVASHH
                     SPEFVVNVKETGKILLVNYEDLSNLKVRDIDAARFLHDGGFDSTGRYFLVAANAVHQI
                     AVVDTEDGELEAIIDTGGQTPHPGRGANFIHPEFGPVWATTHLGDWTISVIGTDPEGH
                     PENAWKVVQMLEGLGGGSLFLKTHPNSRNLWVDTPLNPEPSIASSVAVFDLDNLDAGY
                     EELPIGEWSGITEGVRRIVQPEYNQDGTEVWFSVWNAQDQESAIVVVDDKTRTLKHVI
                     KDPRLITPTGKFNVYNTINDVY"
     gene            4362..4628
                     /locus_tag="P5558_20695"
                     /pseudo
     CDS             4362..4628
                     /locus_tag="P5558_20695"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5779..>6043
                     /locus_tag="P5558_20700"
     CDS             5779..>6043
                     /locus_tag="P5558_20700"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY26800.1"
                     /translation="MEPLIFILWCYVALALLFLGRGVFCGWLCPFGALQELTNKLARL
                     FRVPQLALPFGLHEKLWPTKYIIFLGLLALSLHAMSAATLAAEV"
BASE COUNT          844 a         2125 c         2113 g          961 t
ORIGIN      
        1 ggttggcgtc gcgcaccgag atccagaccg cctcgccctt gggcgtgaac tccatgtgca
       61 gcacgccggg gccgggccgc agctcctcga cgatctcgcc agatggcacg tcgatcacct
      121 ggatgacgtc gttgtcggga tgggcgaagt tgacccagac ctgccggccg tccggccggg
      181 cgatggcgaa gaccggctgg ctgtggaccg ggatgcggtc gatctccctg aaggtcgccc
      241 gatcgacgac gaggagctcg tgccggccga tggcgggcag atagagccgg tcgccggcga
      301 cgctccagcc ctcgagatgc ggcatcttgt agaccggcag gggctcctcg cccttgccgt
      361 agccgtcgag gatgcgccgg ggcgcggcgc cctcgtccca gagatcgagc acgacaaggc
      421 cgtcctcgcc gaagaggccg gcgatataat ggtgcccgtc cggggtgagc agcgcatcgt
      481 agggcagctt gccgaccccg gcgaagcgct ccacttcggg agcggccccg tcctcgagcc
      541 cgaccgtcca gatcgcatcg gcatcgtaga gggcgaagac gaagcggttg tcggcgatct
      601 cctcgatccc gaccaccttg gagcgcgcgc cgtcggcccc gtaggtcgag ggcacctcgg
      661 cgacgggcga gaggtcgtcg cttctgaaga agcgcacgcc gcccggctcg taattggcga
      721 cggcgacgat ccttccgtcc tgcgagatgg caccgccgat gctgttgccg gcctggacga
      781 cccgagcagc gatctcgccg gtcaggagat cgaccttgct gagcccgcca tcgcggccga
      841 agacgaaggc atagcggccg tcgcgcgaga agacgaccga cgcgtggctc agatcgccca
      901 ggcccgccac ccggccgatg gcctgccgct cggtcgtgtc gacgatcagg atcgagtctt
      961 cggcccgctc gacgacgacg ccgaggtcgc cggtgccgcg caagggcggg ctggtggtgc
     1021 aggcggcgag gccgagcacc agtgccagga gcgcaacgaa gcgtcgctca gcgcgcatcg
     1081 ccgatcccct cctgcaggcg accgaccagc cagaccacct cgtcctcggg cagcagcccc
     1141 cgccagggcg gcatcggccg gccgggcacg ccgtcgagaa tgatggcgac caggctctcg
     1201 gccggctggt ccgcgagcgc gtcgggcagg agcggtggcc cgagcccgcc cttcagcgtg
     1261 aggccatggc acgagccgca atcatgacgc aggagatgga tgagctcgtc ctggcgttgc
     1321 agggaaggtt cggcagcggc ggcggagccg gccgggcccg cgatggcgac gcccccgatg
     1381 acgaggccga gcgccagccg gtcaacccag ctggcggcag gcatccgcct cctcccgaag
     1441 gccgctcttg gcggccagga tgtcgccgag ggcgacgacc gagccgaccg cgagcagcac
     1501 cggtgccgtg agcccggccg cctcgaccgc cgccggcagg tccgccagcg tcgcccggca
     1561 gatctgctcg cccggctggg tcccggacgc gatggcgacg gccggtgtcg agggggcgag
     1621 gccggcgccg atcagcccgt ccctgaaccg ggcgaggctg gcgagcgcca tgtagacgac
     1681 gagcgtggtc gcgggatcgg cgagcgtgcg ccagtcgatg tcgaggtcgt ccgattcgca
     1741 cgtatggccg gtgatcagcc tgaccccggt cgccatgccg cgatgggtga gcggaatgcc
     1801 ggcggcggcg agcacacccg aggccgccgt gatgccgggc accacctcga aggggacgcc
     1861 gtgcgccacg agatgcaggg cctcctcgcc gccccggccg aagacatagg gatcgccgcc
     1921 cttgagccgg acgacacggc ggccggcgcg ggcgagccgg acgaggagcg cgttgatcgc
     1981 gtcctgcgcg agcaggtgct cgcccggcgc cttgccggcg cagatccgct gcacgccggg
     2041 cgggatcagg gcgaggatgg cggccgagac gagccggtcg tagacgacca cgtcggccgt
     2101 ctggaagtgg cggagcgcct tgagggtcaa gagctccgga tcacccggtc cggcgccgac
     2161 cagtgcgact gaggctttct cgggcatgcc tgtggctccg ccggacaaca agacccggcc
     2221 ggggcggtgc tgggcaccgc cccggccgtc gctcagtaga cgtcgttgat ggtgttgtag
     2281 acgttgaact tgccggtcgg cgtgatcagc cgcggatcct tgatcacgtg cttgagggtc
     2341 cgcgtcttgt cgtcgaccac gacgatggcc gattcctggt cctgggcgtt ccagacggag
     2401 aaccagacct cggtgccgtc ctggttgtac tccggctgga cgatgcggcg cacgccttcg
     2461 gtgatgcccg accactcgcc gatcggcagc tcctcgtagc cggcgtcgag attgtcgaga
     2521 tcgaagacgg cgaccgacga ggcgatgctc ggctccgggt tgagcggcgt gtcgacccag
     2581 agattgcgcg agttcggatg ggtcttcagg aagagcgagc cgccgccgag gccttccagc
     2641 atctgcacga ccttccaggc attctccgga tggccctcgg gatcggtgcc gatcaccgag
     2701 atcgtccagt cgccgagatg cgtcgtggcc cagaccggcc cgaactcggg atggatgaag
     2761 ttcgcgccac ggcccggatg cggcgtctgc ccgcccgtgt cgatgatcgc ctcgagctcg
     2821 ccgtcctcgg tgtcgaccac ggcgatctgg tgcacggcat tggccgccac caggaagtag
     2881 cggccggtcg agtcgaagcc gccgtcgtgc aggaagcgcg ccgcgtcgat gtcgcggacc
     2941 ttgaggttcg acagatcctc gtagttgacg agcaggatct tgcccgtctc cttgacgttc
     3001 accacgaact cggggctgtg gtgcgaggcg acgatcgagg ccacccgtgg ctccgggtgg
     3061 aactcctgct cgcccaccgt gtagcctctg gtcgagacga ccttcaaggg ctccagcgtg
     3121 gtgccgtcgg cgatgacgta atgcggcggc cagtaggcgc cggcgatgac gagcttgtcc
     3181 tcccagccct ccgctttcga gctctcgacc gagcgcgcct cgatgccgat cttgagctcg
     3241 gccacgaccg cgggcgggtc catccagaga tcgatcatgc tgaccagggc gtcgcggccg
     3301 atgacgtaga ggtagcggcc ggatgccgag agcctggaga tgtggacggc atagccggtc
     3361 ggcaggatcg tcttgatctc gtaggtggca ccgtcgatca gcgccacctc gccggaatcg
     3421 cgcaacgtga cggagaagag gttctctagg tcgaggtcgt tctgcttctc ggtcggccgc
     3481 tccgccacgg gcaccagcac gcgccagttc tcgcgcatgg tctccatgcc ccattcgggc
     3541 ggctgcggcg gctcgtggaa gaggtagcgg gccatgatat tgacctcctc gggcgtgaga
     3601 tcgcccgagg tgccccagtt cggcatgccg cccggcgagc cgtactcgat gaaggcgtgc
     3661 acgtagtcgg cacccttctc cttcatgatg tccggcgtca gcggccggcc ggtggcgccc
     3721 ttgcgcagca cgccgtggca gccggcgcag cgctggaagt agatcacctg ggctcgctgg
     3781 aagtcgtcgg tcgagagcgg ctcgacgccc atcgcctgga gctccggggc gaggcccggc
     3841 tgcgccagct tcatctcgct cagcacgttg agctcgggca cgtaggtcgt gccggccggc
     3901 ggcgtatgcc cttcgagcgc cttcggatcc ggctgctcct gcgcgacgcc catcggcgcc
     3961 gccatcaagg cccacgcacc gagcgccgtc gccgccagaa gtgcagtgcg tcccgtcatc
     4021 ttgcctctcc catatcgcct gcatcagggc accttgacgc ggacgctagc cgccgggggc
     4081 cacgcggtcc ttgacttgcg tcaaggagcc tcgcgccaaa gccccctagc gtcgggccca
     4141 tgcacgaagg acgaggcccg gtgactggac ggcggggcgt tgcgggagtg ccggggcccc
     4201 gcctcatggc cctggccctg ctcctctggt gcgccgcgct cgtcgcggtg ccggccctga
     4261 gccgggcgca ggacgcgccg tccctggcgc tggcgcggga cgtctttgcc ggggccgagg
     4321 gggtcggcgc ctacgcgggc gagccgccgg cggccgacgt catgatcggc ggccggctcg
     4381 ccggctatct cctctccacc cgcgccgtcc tcggctcggt cggctatagc gggcggccct
     4441 tcgaggccgt gctcgggctc gacctcgagg gccggatcac cggggccagg ctggtctccc
     4501 acaacgagcc cgtgctcgtc atcggtgtcg agccggccga gctcgacgcc ttcatgggac
     4561 agttccgggg tcttcccgtc gacgcgacga cgcatcttcg gggcagcacc ggcgaggccg
     4621 gtgtcgacgc gatcagccgg gccaccgtct cctccgtcct cttcgccgac gccattctcc
     4681 gcgccgcccg ggcggtggcc ctgagccgcg gcatcatcga gccggaagcg gcgaccgccg
     4741 gcctggatct ggatcgcttc gaggagctgg agtggccggc cctcagcgac accggcctgg
     4801 tgagccggct cctcctgacc aatggcgaca tcgccagagc gctcctcgag cgcggccggc
     4861 cgccaccggc cggcccgggc ttcgaggacc caacggccac cttcgtcgac ctccgggtgg
     4921 cgctcgccac gccggcgatg accggccaaa acctgctcgg cgaggcgcgc catctggcgc
     4981 tggtccacgc cctccagccc ggcgaccagg ccatcatcgt cctcgccagc ggccgttatt
     5041 cggtcaaggg cacgggcttc gtcaggagcg gcgtcttcga ccgcctgcag gtgctgcagg
     5101 gcgagaaggt catccggctg gaagccgagg gccacacccg catcgacgag ctcgtgatcg
     5161 acggtgctcc ggagtttcgc gagatgggct ggttcgtgct gccggcggcg agcggcttcg
     5221 acccgctcca gccctggagc ctccggctga tcgtggccca gccgaccgag gcgggcgagg
     5281 tgctgctcga tttcgtcctg ccggtgacgg cccccacggc actcgtcgcc cggcccgtcg
     5341 aggcgaagcc acgaggctgg ctcgccggcc ttttggacgc cgccctggag cccgacggct
     5401 cgctggtcgt cagccccggc ctctggctcg aggtctgggc cgcgcgctcg ctcgatatcg
     5461 cgcttctggt cctgatgctc gccgtccttt ccgtggccct ggttttccag gacgcgctgg
     5521 tgaagagaat agggttctat cgccgcttcc gcgtcgcctt cctcgcggtc acccttttct
     5581 ggctcggctt cgccatgggt gcccagctct cggtggtcaa catcctgacc ttcttcaaca
     5641 gcctgctcac cgggttcagc tggcaggtct tcctgatgga gcccttgatc ttcatcctct
     5701 ggtgctatgt cgccctggcg ctgctcttcc tcgccgcgcc cgaggtagag cagcgccagg
     5761 gcgacatagc accagaggat ggagcccttg atcttcatcc tctggtgcta tgtcgccctg
     5821 gcgctgctct tcctcgggcg cggcgtcttc tgcggctggc tctgcccgtt cggtgccctg
     5881 caggagctga ccaacaagct cgcccgcctc ttccgcgtgc cacagctcgc cctgcccttc
     5941 gggctgcacg agaagctgtg gcccaccaag tacatcatct tcctcggcct cctcgccctc
     6001 tcgctccatg cgatgagtgc tgccaccctc gccgccgagg tgg
//
LOCUS       DAOTFI010000271         6006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_15649_length_6006_cov_7.508654, whole genome shotgun sequence.
ACCESSION   DAOTFI010000271 DAOTFI010000000
VERSION     DAOTFI010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15649_length_6006_cov_7.508654"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /gene="dctP"
                     /locus_tag="P5558_20705"
     CDS             complement(<1..469)
                     /gene="dctP"
                     /locus_tag="P5558_20705"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY26801.1"
                     /translation="MKLTTTLLTGAAMLALATSLASARELTLGMQDNEASNVFLGAQE
                     FAARLADLSGGELTVTLFPSSQLGDFKAMVAQVQAGELDMVITGYPDMSYTIPELNLV
                     GAPYVARDFEHLQEIVAGPWGQKMAAEFEAQGVKLLDVWYYGTRQTTANKPINS"
     gene            complement(521..1243)
                     /locus_tag="P5558_20710"
     CDS             complement(521..1243)
                     /locus_tag="P5558_20710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRY26802.1"
                     /translation="MHEPKRTPDLIAQTLVGEIRAGTIELDEALPTERELCERFAASR
                     PTVREALAQMQLRGYVTAAAGRRPRASRPSIANILKGAGEQIRDLLGDAESGAHLEQM
                     RLFIETGAVREAATRAANLQLTKLQGALERNFEAIGTSAFAPTDIAFHRVLVSISGNP
                     VILTLHDMFVSSMIAHRPPTDDPERYDRIAYDEHRLIYEAVRDNDVTAATDVMERHLA
                     RSYRARLGAPRLSTTSTQTHEL"
     gene            1443..2339
                     /locus_tag="P5558_20715"
     CDS             1443..2339
                     /locus_tag="P5558_20715"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY26803.1"
                     /translation="MTKLLAGMYAALMTGLDDAGEFDPKRQRRLDEYVLRQGLTGLYV
                     GGSSGESGLLGADELLAQQAVVAESAKGSGARLIAHVGMPSLRDSIRLARQAKELGYE
                     GLSALPPHSYPFSDDEIVAYYRELAAATDLPLIVYEVPIRTGRPIPLEALLRILDLSN
                     VAGIKFTSTDLFKLAMLRRRRPEHTYFFGFDEIYLSGAALGADGGIGTTYNLLGRLYA
                     ALDDAIRAGDLARAQELQDLSQIFVEALLETGVLPGMKAAFRAIGIDCGPTRAPMALR
                     LADGDARMAAVLAQPELKAWLA"
     gene            2411..3571
                     /locus_tag="P5558_20720"
     CDS             2411..3571
                     /locus_tag="P5558_20720"
                     /inference="COORDINATES: protein motif:HMM:NF010730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HRY26804.1"
                     /translation="MTRRMGLCAALASVAIMAGMGAAVAEEWPDLPVGIKNGIAAQIG
                     DRLIVGLGSAGTDILALDLNDRAAGWQKLASFDDPAPSQPAAAVSGGALYVFSGSGKA
                     DAAAASPVIFDTVHRYDPQADAWETLGTTTPAGLLGASAMTLGDGRIAIIGGYNKELF
                     DRYIADVTAIDKEAEPERWNAVVNDYMGMTPKEYRWNDRVLLFDPADKSWSDLGANPE
                     LPNTGAAVVETAPGEFLVINGEIKPGLRTDAVKRVTFGADGATWQAVAPVPAPAGSEL
                     QEGLAGAYAGLSHDHVIVAGGANFPGARQNAEAGNWFAHEGLSKHWASDVFVLDGEQW
                     QEVGELPLGLAYGASFSVDDTLLVVGGEDATGTARMDVFALEWNGDKLAILD"
     gene            3886..4737
                     /locus_tag="P5558_20725"
     CDS             3886..4737
                     /locus_tag="P5558_20725"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRY26805.1"
                     /translation="MKQSATHRAFAILELLVGLPAGLGLGEICARLDIPKSIAHRLLA
                     LLAERGLVRQDPATGHYALTLEFALMGARHLAGIGLVDVGQPILDRLAAATGELCRLA
                     VVEGEGMVWVAKAQGARYGLRYDPDAGTRVVLHATATGKAWLATLPEAEAMRIVEATG
                     FVTPRHFGPNAISEGRAFSVELARTRERGYGLALEEGEAGIAAVAVVVRRSTEASAPA
                     VGTLSVAGPVTRLTPDRCRQLANGLGTAAAELSALWPIRLPLGGVAARSTAQGHQPDE
                     GAMRHVA"
     gene            4727..5185
                     /locus_tag="P5558_20730"
     CDS             4727..5185
                     /locus_tag="P5558_20730"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY26806.1"
                     /translation="MSLDPQPRYPGPLPLAEALTALNLAEASDAAFTRDGPRADVEYR
                     DLGLAAATNGRIGAKHIRAIAPFGQETGWHWHDMTGHFVFVLKGWLTFRFAGVGEPVT
                     VRAGGCLSQPAGVAHNVVGRADDLEIIEINMPATYGTVDLAGPDGGTHGS"
     gene            5175..>6006
                     /locus_tag="P5558_20735"
     CDS             5175..>6006
                     /locus_tag="P5558_20735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26807.1"
                     /translation="MAAEPADLLAPNETGPSRVLAWLRKRAGLIAVFVLLFLLWEYAV
                     WLFGVKAYLLPPPSLVWSELLERWPTVASGAWVTTQEIIGGYLLAVVISVPLALAVAK
                     SAFVEESIYPVVVFLQIVPKIAIAPLFIIWLGFGYAPKLLIVFLLCFFPIVVSSVAGF
                     KSVDPEIRDFARTTGASAWKMFKTIQLPQALPDIFTGLKVGAALAATAAVVAEFVAAD
                     RGLGYLLLQYNGQFETPMVFAIIIVLSLIGLAVYYVVELIERLVIPWHVSQRPAGVTG
                     V"
BASE COUNT          898 a         2092 c         2029 g          987 t
ORIGIN      
        1 tcgaattgat cggcttgttc gcggtggtct ggcgggtgcc gtagtaccag acgtcgagca
       61 gcttgacgcc ctgcgcctcg aactccgccg ccatcttctg gccccagggg ccggcgacga
      121 tctcctgcag atgctcgaag tcgcgggcga catagggtgc gccgacgaga ttgagctcgg
      181 ggatcgtgta gctcatgtcc ggatagccgg tgatgaccat gtcgagctcg ccggcctgga
      241 cctgggcgac catcgccttg aagtcgccga gctgcgagga ggggaagagc gtcacggtga
      301 gctcgccgcc cgagaggtcg gcgaggcggg cggcgaattc ctgcgcgccc aggaagacgt
      361 tggacgcctc gttgtcctgc atgccgagcg tcagctcgcg cgccgaggcg agggaagtcg
      421 ccagcgccag catggcggca ccggtcagaa gggtcgtggt gagcttcatc gtttcctccg
      481 ttcttgatcg atcagggcaa gaaggcgtct tgccggcgcg tcagagctca tgcgtctgag
      541 ttgatgtcgt cgaaagccgc ggggcgccga gccgggccct gtaggaccgg gccagatggc
      601 gctccatgac gtcggtcgcc gccgtcacgt cgttgtcgcg gaccgcctcg tagatcaggc
      661 ggtgctcgtc ataggcgatg cggtcgtagc gttcggggtc gtcggtcggc gggcgatggg
      721 cgatcatcga gcttacgaac atgtcgtgca gggtgaggat caccgggttg ccggagatcg
      781 agacgaggac gcggtggaag gcgatgtccg ttggcgcgaa agcactcgtg ccgatggcct
      841 cgaaattgcg ctcgagcgca ccttgcagct tggtgagctg gaggttggcg gcccgcgtcg
      901 ccgcctcgcg caccgcaccg gtctcgatga acaggcgcat ctgctccaga tgggcaccgc
      961 tctctgcgtc gccgaggagg tccctgatct gctcgccggc acccttgagg atattggcga
     1021 tggaggggcg gctcgccctg ggccgccggc cggcggcggc ggtgacgtag ccgcgaagct
     1081 gcatctgcgc caaggcctcg cgcacagtcg gccgggaagc ggcgaaccgc tcgcacagct
     1141 cgcgctcggt cggcagcgcc tcgtccagct cgatcgtgcc cgcccggatc tcgccaacca
     1201 gcgtctgggc gatcagatcg ggcgttcgct tcggctcgtg catgtcacgg atccacgcgc
     1261 atggcccctg ccgtcctcga tgcaaacatc gccgatatgc tgggccgtct caccagattg
     1321 gaataatggt attacaaaag aaaattcttg tcaaaccaaa actgcgacct atgctccagc
     1381 ccatcgatgc cggatgctcg cgggcggggt gtcgtgaggc tggtgcgtga aaggatgcga
     1441 ggatgaccaa gctgctggcg ggcatgtacg cggcccttat gaccgggctc gacgatgccg
     1501 gtgagttcga cccaaagcgc cagcgtcgcc tcgacgagta cgtgctgcgc cagggcctga
     1561 ccgggctcta tgtcggcggc agcagcggcg aatccggcct cttgggcgcc gacgagctgc
     1621 tcgcccagca ggcggtcgtg gccgagagtg cgaagggcag cggtgcccgg ctgatcgccc
     1681 atgtcggcat gccgtccttg cgcgattcga tccgcctcgc ccgccaggcg aaggagctgg
     1741 gctacgaggg cctgtcggcg ctgccgccac actcctatcc gttcagcgac gacgagatcg
     1801 tcgcctacta ccgcgagctc gccgccgcca ccgacctgcc gctgatcgtc tacgaggtgc
     1861 cgatccgcac cggccggccg attcctttgg aagcgctcct gcgcatcctc gacctctcga
     1921 acgtcgccgg catcaagttc acctccaccg acctcttcaa gcttgccatg ctgcgccgcc
     1981 gccgcccgga gcacacctat ttcttcggct tcgacgagat ctacctcagc ggcgccgcgc
     2041 tcggcgccga tggcggcatc ggcaccacct acaacctcct cggccggctc tacgcagcac
     2101 tcgacgacgc gatccgcgcg ggcgacctcg cccgcgccca ggagctgcag gacctctccc
     2161 agatcttcgt ggaagctctc ctcgagacgg gcgtgctgcc cggcatgaag gcggccttcc
     2221 gcgccatcgg catcgactgc ggcccgaccc gcgcccccat ggcgctgcgc ctcgccgacg
     2281 gtgacgctcg catggccgcc gtcctcgccc agcccgagct gaaggcctgg ctcgcctgaa
     2341 cggctcctcg ccggacgcgg caacccgcct ccggcaccac aattcaaacc gacccacggg
     2401 aggatcgttg atgacacgtc gcatgggact ttgcgccgcc ctggcgagcg ttgcgatcat
     2461 ggccgggatg ggagcagccg tggccgagga gtggccggat ctgccggtcg ggatcaagaa
     2521 cgggatcgcg gcacaaatcg gcgaccgcct gatcgtcggg ctgggcagcg ccggcaccga
     2581 catcctcgcc ctcgacctga acgacagggc cgccggctgg cagaagctcg cctcgttcga
     2641 cgacccggca ccgtcccagc cggctgccgc cgtctcgggc ggcgccctct acgtgttcag
     2701 cggctcgggc aaggccgacg ccgcggccgc gtcgcccgtc atcttcgaca cggtccatcg
     2761 ctatgacccg caggcggacg cctgggaaac gctgggcacg accaccccgg ccggactgct
     2821 cggcgccagc gccatgactc tcggggatgg ccgcatcgcg atcatcggcg gctacaacaa
     2881 ggagctcttc gaccgctaca tcgccgatgt caccgccatc gacaaggagg ccgagcccga
     2941 gcgctggaac gcggtcgtca acgactacat ggggatgacg ccaaaagaat accggtggaa
     3001 cgacagggtg ctgctcttcg atccggcgga caagagctgg agcgacctcg gtgccaatcc
     3061 cgagctgccg aacacgggtg ccgccgtcgt cgagacggca ccgggcgagt tcctggtcat
     3121 caacggcgag atcaagccgg gcctgcgcac cgacgcggtg aagcgtgtca ccttcggcgc
     3181 cgacggggcg acgtggcagg cggtggcacc cgtgcccgcg cccgctggga gcgagcttca
     3241 ggaaggcctc gccggggcct atgccgggct ttcccatgat cacgtgatcg ttgccggcgg
     3301 cgccaacttt ccgggtgccc gccagaacgc cgaggccggc aactggttcg cgcacgaagg
     3361 gctctcgaag cactgggcgt cggacgtctt cgtgctcgat ggcgaacaat ggcaggaggt
     3421 cggcgagctg cccctgggcc tcgcctacgg cgcctccttt tccgtcgacg acacgctgct
     3481 cgtggttggc ggagaagacg ccaccggcac ggcccgcatg gacgtcttcg ccctcgaatg
     3541 gaacggcgac aagctcgcca tcctggactg actacgagag ggccgcgccc aaaacgctac
     3601 ggctcgcggt gcggtggtca tggtgctgag cgaataggcc atggccgagt tggggagggc
     3661 gccggccagc aggtgggcga gcgcccggag accccccggt gggaagcatc atctgatgac
     3721 ctcatccctt cggggccgtc gtctcggcgc ccggcatcag ggggccggcc aatccctcgt
     3781 cgagccaacc ggatcgctcg cttgcatctc gccgatccgg cccggcgttg acacgccggc
     3841 gggatgatcc ataaacggaa tggcattcca gtttttcggc agtcgatgaa gcagtccgcc
     3901 acccaccgcg ccttcgccat cctcgagctg ctcgtcggcc tccccgccgg gctggggctc
     3961 ggcgagatct gcgctcggct ggacattccc aagagcatcg cgcaccgcct gctggccctg
     4021 ctcgccgagc gtggcctggt ccgccaggac ccggcgaccg gtcactacgc cctgaccctc
     4081 gagttcgcgc tcatgggcgc acgtcatctc gccggcatcg gtctcgtcga tgtcggccag
     4141 ccgatcctcg accggctggc ggcggccacg ggcgagctct gccggctcgc cgtggtcgaa
     4201 ggggagggga tggtctgggt cgccaaggcg cagggtgcgc gctacggcct gcgctacgac
     4261 cccgatgccg gaacccgtgt cgtcctccat gcgacggcga cgggcaaggc ctggctcgcc
     4321 acgctgcccg aggcggaggc gatgcgcatc gtcgaggcaa ccggttttgt caccccccgc
     4381 catttcggcc ccaacgccat cagcgagggc agggcgttca gcgtcgagct cgcccgcacc
     4441 cgcgagcgcg gctatgggct cgccctcgag gaaggcgagg cgggcattgc cgccgtcgcg
     4501 gtggtcgtgc gccgctcgac cgaagccagc gcgccggcgg tcggcacgct cagtgtcgcc
     4561 gggcccgtca cccgcctcac gcccgatcgc tgccgccagt tggcgaacgg cctcggcacg
     4621 gcagcggccg agctcagcgc cctctggccg atccgcctgc cgctcggcgg tgtcgctgcg
     4681 cgttccactg cccagggcca ccagccggac gagggagcca tgcgccatgt cgcttgaccc
     4741 acagccccgc tatcccggac cgctgccgct cgccgaggcg ctgaccgcgc tcaacctggc
     4801 cgaggcgtcg gacgcagcct tcacccggga cggaccgcgc gccgatgtcg aatatcgcga
     4861 tctcggcctg gcggcggcga cgaacggcag gatcggtgcg aagcacatcc gggccatcgc
     4921 gcccttcggc caggagacgg gctggcactg gcacgacatg accggccact tcgtcttcgt
     4981 gctgaagggc tggctcacct tccggtttgc cggcgtcggg gagccggtta cggttcgggc
     5041 cggcggctgc ctgtcgcagc cggccggggt ggcgcacaac gtcgtcggcc gcgcagatga
     5101 tctggagatc atcgagatca acatgccggc gacctatggc acggtcgatc tcgcggggcc
     5161 cgatggcggc acgcatggca gctgaaccgg ccgacctcct ggcgcctaac gagaccggcc
     5221 caagccgcgt gctcgcctgg ctgcgcaagc gggcgggcct gatcgccgtc ttcgtcctgc
     5281 tcttcctcct ttgggaatac gcggtctggc tcttcggcgt gaaggcctac ctcctgccgc
     5341 cgccgtcttt ggtctggagc gagctgctcg agcgctggcc gaccgtcgcc agcggcgcct
     5401 gggtgacgac ccaggagatc atcggcggct atctgctcgc cgtggtgatc agcgtgccgc
     5461 tggcgctggc cgtcgccaag tcggccttcg tcgaggaatc gatctacccg gtcgtggtct
     5521 tcctgcagat cgtgccgaag atcgcgatcg ccccgctctt catcatctgg ctcggcttcg
     5581 gctacgcgcc gaagctgctg atcgtctttc tcctctgctt ctttcccatc gtcgtctcga
     5641 gcgtcgcggg cttcaagtcg gtggacccgg agatccgcga cttcgctcgc accaccggcg
     5701 ccagcgcctg gaagatgttc aagacgatcc agctgccgca ggccctgccc gatatcttca
     5761 ccgggctcaa ggttggtgcc gcactcgccg cgacggctgc cgtggtcgcc gagttcgtcg
     5821 ccgccgatcg cggcctcggc tacctcctgc tccagtacaa cgggcagttc gagacgccga
     5881 tggtgttcgc catcatcatc gtgctgagcc tgatcgggct cgccgtctac tacgtcgtcg
     5941 agctgatcga gcggctcgtc attccctggc acgtgtcgca gcggccggcc ggggtgacgg
     6001 gagtct
//
LOCUS       DAOTFI010000272         5957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_15829_length_5957_cov_6.489326, whole genome shotgun sequence.
ACCESSION   DAOTFI010000272 DAOTFI010000000
VERSION     DAOTFI010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15829_length_5957_cov_6.489326"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="P5558_20740"
     CDS             <1..972
                     /locus_tag="P5558_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRY26808.1"
                     /translation="LLALLLILAGLALVLPEQAKASLGFTASSLLDVAPFLLASVALA
                     AYAKATGADNLIARAFSGRTAPMILAASLTGALSPFCSCGVIPLIAALLAMGVPLPAV
                     MAFWLASPLMDPSMFALTTGTLGLDFALYKTFAAIAMGLFGGFGMALLVRNGAFAEPL
                     RPEVGNGGCGGGRVRTPKPVHWRFWDEPARRLTFGRSALDTTLFLGKWLTLAFLLESL
                     MLAWVPAELVASTLGGEGIGPVFLAVLVGVPAYLNGYAALPLIGGLIDQGMAPGAGMA
                     FLIAGGVTSLPAAIAVWALARPPVFLAYLGFSVTGALVLGLLFTLVG"
     gene            1037..2185
                     /locus_tag="P5558_20745"
     CDS             1037..2185
                     /locus_tag="P5558_20745"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HRY26809.1"
                     /translation="MRGGLMLAAGLAAGLLGGVASGQEAPVKLGAVLPFSGGLELFGG
                     QARLGIDLAVKEINDGGGIQGRPVEVIYEDNQTDPRTSVERATKLTRGDGVIAVLGPI
                     TSNARDAMLPTMERAKVPLLYATNYEGGACSRYVFAFNSVPNQELAELVPYLAQNRGR
                     KFFMFGADYVWPQKMFETAATLVAEAGGEVVGTEFTPWGVTEFAPVVRRIEESGADVL
                     LFALPGADGITFIRQAEDFGLLEDVTVGFLGFSEDYLGAFGEGKAQDMWVTVPFAAAD
                     PSEGVQYFTGRLAEMAGPDVVASHYTMTHYNAVIAVAEALEGAETIDAEALVDGLEGL
                     EIETPTGLLSIGAEDHHVTMEMLLAHTVGDGLEVVDRLGRIAPEAGCN"
     gene            2457..3167
                     /locus_tag="P5558_20750"
     CDS             2457..3167
                     /locus_tag="P5558_20750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26810.1"
                     /translation="MTLLAVEGLEKRFGGVQALAGLSLSLDEGGLLCLLGPNGCGKTT
                     FFRVLSGETAADAGRIDFADRRLEGEGPAGRARLGIARKLQTPGLFAALTVAEHLALA
                     AASPAAHGPVDAAALLARVGLGEHAETPAGRLSHGQRQWLEIAMLLALRPRLLLLDEP
                     TAGMTRAETRRTAALLRELNDKDGVAAIVIEHDMAFVRELDVAVAVMLQGRVVATGSY
                     AEVGRDPLVRELYLGKRA"
     gene            3167..3868
                     /locus_tag="P5558_20755"
     CDS             3167..3868
                     /locus_tag="P5558_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26811.1"
                     /translation="MAALFEVRGLVAGYGGARILDGVDLDVEAGGITCLLGRNGTGKT
                     TTLKALMGLLPAAAGSVRLDGQELAGLAAHRIARAGIGWVPQGRSIFAGFSVEENLAI
                     GGLGHGARLDDGLMERFPVLAERRRQDASTLSGGEQQQLAIARALVGRPRLLLLDEPT
                     EGIQPSLVDRLAVTLAVIARQDGIGLLLVEQNLDLALGLADRVLVMEKGRIELVVEAA
                     GLADALPALEARLSI"
     gene            3874..4740
                     /locus_tag="P5558_20760"
     CDS             3874..4740
                     /locus_tag="P5558_20760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY26812.1"
                     /translation="MGADLVLALLDAANFVLAIILVAMGLMVVFGMMRIINMAHGELF
                     LLGAYALLVVQGLGGSFWLALVLGPLVVGLVGLLLEVLVIRHLYGRVLDSILATWGLS
                     LLIRQTVILWFGPQSRVVAAPFDLRLDILGQPFPAYRLFIMGVSLAVILFCFWLFHRT
                     RLGLAARATIADRATAEAMGIDSRGLDRLTFAFGAALAGLAGVVMAPLLSVDPQMGLG
                     FLIPAFLAILTGGAGELMGVVTGGVVVGGSQSLVARVASPAVAELVVLGLAILVIRLL
                     PNGLVGQFRRRR"
     gene            4737..5720
                     /locus_tag="P5558_20765"
     CDS             4737..5720
                     /locus_tag="P5558_20765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRY26813.1"
                     /translation="MSRKRLVQGLVWLGLTLLPLFIGEWQTLQLAQFLTYGLFAMSLA
                     LAWGRLGLLSFGHALFFGLGAYAMALATMGRLPGLEAFPSSWLGLALACLVPWATAQL
                     LGFFLFHGRGLRGAYFGITMLALAVLAERLTQGTAWLGGLNGLIGVPPLALGLDGGGP
                     ALLDPLPTYWVMLGVAFAVFLGLEMLLASSRGLAMTAVRENEDRLGFLGYDVAAIKRH
                     GFALSAAIAGLAGALFASQFGFVSPKLIGFGLSAEVLIWTALGGREMLLAAFFGALLL
                     SWTETVLAGLLGAYWLLALAALLVLSVLFFERGFVAGILTLLSRGRRPGRR"
     gene            complement(5680..>5957)
                     /locus_tag="P5558_20770"
     CDS             complement(5680..>5957)
                     /locus_tag="P5558_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRY26814.1"
                     /translation="AIARRQMTRGFGGMLSILVHGGPEAAKRIASATRLFLPATSLGG
                     VESLIEHRKAVEGPNSIVPGNLLRLSVGIEDVGDLIADLDAALGTAR"
BASE COUNT          703 a         2041 c         2148 g         1065 t
ORIGIN      
        1 cttcttgccc ttctgctgat cctcgccggt ctcgccctcg tcctgccgga gcaggcaaag
       61 gccagcctcg gcttcaccgc ctcgagcctg ctcgacgtcg cacccttcct tctcgcctcg
      121 gtggcgctcg cggcctatgc caaggccacg ggcgccgaca acctgatcgc ccgcgcgttc
      181 agcggccgca ccgcgccgat gatcctggcg gcgagcctga cgggtgcgct ctcgcccttc
      241 tgctcctgcg gcgtcatccc gttgatcgcg gctctcctgg ccatgggcgt gccgctgccg
      301 gcggtcatgg ccttctggct cgcctcgccg ctcatggacc cctcgatgtt cgcgctgacc
      361 accggcacgc tcggcctcga cttcgccctc tacaagacct tcgcggcgat cgcgatgggc
      421 ctcttcggcg gcttcggcat ggcgctgctg gtcaggaacg gtgccttcgc cgagcccctg
      481 cggccggaag tgggcaatgg cggctgcggc ggcgggcggg tgcgcacgcc caagccggtg
      541 cactggcggt tctgggacga gccggcgcgc cggctgacct tcggccggag tgccttggac
      601 acgacgctct tcctcggcaa gtggctgacg ctcgcgtttt tgctcgagag cctgatgctg
      661 gcctgggtcc cggccgagct ggtcgcctcg acgctgggcg gcgagggcat cggccccgtc
      721 ttcctggcgg tgctggtggg ggtgccggcc tacctcaacg gctatgccgc cctgccgctg
      781 atcggcggct tgatcgacca gggcatggcg ccgggggccg gcatggcgtt tttgatcgcc
      841 ggcggcgtca cctcgctgcc ggccgccatc gccgtctggg cgctggcgag gccgcccgtg
      901 ttcctggcct atctcggctt ctcggtgacg ggtgccctgg tcctcggcct gctcttcacc
      961 ctggtcggct gagaggtcgt ttacagcgac ggcccgatct tcctatgctc ccgcattcct
     1021 attggggaga gggacgatgc gtggcggtct gatgctggcg gcgggactgg cggcagggct
     1081 tctcggcggc gttgcctcgg gccaggaggc gccggtcaag ctcggcgccg tgctgccctt
     1141 ctcgggcggg ctcgagctct tcggcgggca ggcgcggctc ggcatcgacc tcgcggtcaa
     1201 ggagatcaac gacgggggcg gcatccaggg ccggccggtc gaggtgatct acgaggacaa
     1261 ccagacggat ccaaggacct cggtcgagcg cgccaccaag ctcacccggg gcgacggggt
     1321 gatcgccgtg ctggggccga tcacctcgaa cgcccgcgac gccatgctgc cgaccatgga
     1381 gcgggccaag gtgccgctgc tctatgcgac caattacgag ggcggggcct gctcgcgcta
     1441 cgtcttcgcc ttcaactcgg tgccgaacca ggagctggcc gagctggtgc cgtatctggc
     1501 ccagaatcgc ggcaggaagt tcttcatgtt cggcgccgat tatgtctggc cgcagaagat
     1561 gttcgagacc gcggccacgc tggtcgccga ggcgggcggc gaggtggtcg gcaccgagtt
     1621 cacgccctgg ggggtcaccg agttcgcgcc ggtggtccgc cggatcgagg agagcggggc
     1681 ggacgtgctg ctcttcgccc tgcccggcgc cgacggcatc accttcatcc gccaggcgga
     1741 ggatttcggc ctcctggagg acgtgaccgt cggctttttg ggattctccg aggattatct
     1801 gggggcgttc ggcgagggca aggcgcagga catgtgggtg accgtgccct tcgccgcggc
     1861 cgatccgtcg gaaggggtgc agtacttcac agggaggctc gccgagatgg cggggccgga
     1921 cgtcgtcgcc tcgcactaca ccatgaccca ctacaacgcg gtgatcgcgg tggcagaggc
     1981 gctcgaaggg gcggagacga tcgacgccga ggcgctggtc gacgggctcg aggggctcga
     2041 gatcgagacg ccgacgggcc tcctctccat cggtgccgag gaccatcacg tgaccatgga
     2101 gatgctcctc gcccacacgg tgggcgacgg gctcgaggtg gtcgaccggc tcggccggat
     2161 cgcacccgaa gccggctgca actgagagcc tgtttgagaa gttttcacgg tgtttcatcg
     2221 acttgcgatc gaccaccggc gcgaagccga gcgcggtcga tgccggggca tcgggcaagc
     2281 tcggcgacaa agtcggagcc gatcgcaagt cgaccgtagg tgaggctggc agcggcacct
     2341 gcttcgtcgt tgatcgaccg acgatgcgcc gcatcgacgg ccgatcaact cctcgcatct
     2401 gctcactgcc agcctcatga aacatcatga aaacttctca agcaggctct gagcgcatga
     2461 cgctgcttgc cgtcgaaggg ctggagaagc gcttcggcgg cgtgcaggcg ctggccgggc
     2521 tctcgctctc gctcgacgag ggtgggctgc tctgtctcct gggacccaat ggctgcggca
     2581 agacgacgtt ctttcgcgtg ctgagcggcg agacggcggc ggatgccggt cggatcgact
     2641 tcgccgatcg gcggctcgag ggtgaggggc cggccgggcg cgcccggctc ggcatcgccc
     2701 gcaagctcca gacgccggga ctgttcgccg cgcttaccgt ggccgagcat ctggcgctgg
     2761 cggcggcctc gccggcggcc cacggcccgg tcgatgcggc ggcgctgctg gcccgggtcg
     2821 ggctcggtga gcatgccgaa acaccggccg gccggctttc gcatggccag cggcaatggc
     2881 tcgagatcgc catgctgctg gcgctccggc cgaggctcct cctgctggac gagccgaccg
     2941 ccggcatgac ccgggcggag acgcggcgga cggcagcgct gctccgcgag ctcaacgaca
     3001 aagacggggt cgccgccatc gtcatcgagc acgacatggc cttcgtgcgc gagctcgatg
     3061 tggcggtcgc cgtcatgctg cagggccggg tggtggcgac cggcagctat gcggaggtcg
     3121 gccgggatcc cctggtgcgc gagctctatc tcggcaagcg cgcctgatgg cggcgctctt
     3181 cgaggtcagg ggcctcgtcg ccggctatgg cggtgcccgc atcctcgacg gtgtggatct
     3241 cgacgtcgag gcgggcggga tcacctgcct tttggggcgg aacggcaccg gcaagacgac
     3301 gacgctgaag gcgctgatgg ggcttttgcc ggccgcggcc ggcagcgtcc gtctcgatgg
     3361 gcaggagctc gccgggcttg cggcgcaccg gatcgcgcgg gccgggatcg gctgggtgcc
     3421 gcagggccgc agcatcttcg ccggcttcag cgtcgaggag aacctcgcca ttggcgggct
     3481 cggtcatggt gcgcggctcg acgatggcct gatggagcgc tttccggtcc tcgccgagcg
     3541 gcggcggcag gacgcttcga ccctctcggg cggcgagcag cagcagctcg ccatcgcccg
     3601 ggccctggtc ggccggccgc gcctgctgct gctcgacgag ccgaccgagg gcatccagcc
     3661 ctcgctggtc gatcggctgg cggtgacgct ggccgtcatt gcgcggcagg acgggatcgg
     3721 gctcctgctg gtcgagcaga acctcgatct ggccctgggg ctcgccgacc gggtgctggt
     3781 gatggagaag gggcggatcg agctcgtggt cgaggcggcg gggctggcgg acgcgctgcc
     3841 ggcgctcgaa gcccggctct cgatctgacg gccatggggg ccgacctcgt cctcgccttg
     3901 ctcgatgcgg cgaacttcgt gctcgccatc atcctcgtcg ccatggggct gatggtcgtc
     3961 ttcggcatga tgcgcatcat caacatggcg catggcgagc tcttcctgct cggcgcctat
     4021 gcgctgctcg tggtccaggg cctgggcggc tcgttctggc tggcgctggt gctggggccg
     4081 ctcgttgtcg ggctggtggg gctgctgctc gaggtgctgg tcatccgcca cctctacggg
     4141 cgcgtgctcg acagcatcct cgccacctgg gggctttcgc tgctgatcag gcagacggtg
     4201 atcctgtggt tcgggccgca gagcagggtg gtggcggcac ccttcgatct ccggctcgac
     4261 atcctgggcc agcccttccc cgcctatcgc ctgttcatca tgggcgtgtc cctggccgtc
     4321 atcctcttct gcttctggct cttccaccgc acgcggctgg ggctcgcggc cagggccacc
     4381 atcgccgacc gcgcgacggc agaggccatg ggcatcgaca gccgcggcct cgatcggctg
     4441 accttcgctt ttggcgcagc gctcgcaggg ctcgcgggcg tggtcatggc gccgctcttg
     4501 agcgtcgatc cgcagatggg gctgggcttt ctcatcccgg ccttcctcgc catcctgacc
     4561 ggcggggcgg gcgagctcat gggcgtggtc acgggcgggg tggtggtcgg cggcagccag
     4621 agcctggtgg cgcgcgtcgc ctcgccggcc gtggccgagc tcgtggttct cggcctcgcc
     4681 atcctcgtca tccggctcct gccgaacggc ctcgtcggcc agttccggcg gcggcgatga
     4741 gccgaaagag gctcgtccag ggcctcgtct ggctcggcct cacgctgctg ccgctcttca
     4801 tcggcgagtg gcagaccctg cagctcgccc agttcctgac ctatggcctt ttcgccatga
     4861 gcctcgcttt ggcctggggc cggctcgggc tcctctcgtt cggccacgcc ctcttcttcg
     4921 ggcttggcgc ctatgccatg gcgctcgcca ccatgggccg cctgccgggg ctcgaggcct
     4981 tcccctcctc ctggctcggc ctcgccctcg cctgcctcgt cccctgggcg acagcacagt
     5041 tgctgggctt cttcctcttt cacgggcggg ggctgagggg ggcctatttc ggcatcacca
     5101 tgctggccct cgccgtgctc gccgagcggc tgacccaggg cacggcctgg ctcggcgggc
     5161 tgaacgggct gatcggggtg ccgcctctgg ccctcggcct cgacggcggc gggccggcgc
     5221 tgctcgatcc gctgccgacc tactgggtga tgctgggcgt ggccttcgcc gtcttcctcg
     5281 ggctcgagat gctgctcgcg tcctcccgcg ggctcgccat gacggcggtg cgcgagaacg
     5341 aggaccggct cggatttctc ggctacgacg tggccgccat caagcggcac ggcttcgccc
     5401 tttcggccgc catcgccggg ctcgccggcg ccctcttcgc cagccagttc ggcttcgtct
     5461 cgcccaagct gatcggcttc ggcctctcgg ccgaggtcct gatctggacc gccctcggcg
     5521 gccgcgagat gctgctggcg gcgtttttcg gcgccctgct gttgagctgg accgagacgg
     5581 tgctggcggg cctgctcggc gcctactggc tcctggcgct cgccgccctg ctggtgctga
     5641 gcgtgctctt cttcgagcgc ggcttcgtcg ccggcatcct caccttgctg tcccgagggc
     5701 ggcgtccagg tcggcgatga ggtcgcccac gtcctcgatg ccgaccgaga ggcgaagcag
     5761 gttgcccggg acgatgctgt tggggccctc gacggccttc ctgtgctcga tcaggctctc
     5821 gacgccaccc aaggacgtgg cggggaggaa gaggcgggtc gccgacgcga tgcgcttggc
     5881 cgcctcgggg ccgccatgga cgaggatcga gagcatgccg ccgaagcccc gggtcatctg
     5941 gcgcctggcg atggcgt
//
LOCUS       DAOTFI010000273         5922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_15971_length_5922_cov_7.215613, whole genome shotgun sequence.
ACCESSION   DAOTFI010000273 DAOTFI010000000
VERSION     DAOTFI010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15971_length_5922_cov_7.215613"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="P5558_20775"
     CDS             <1..928
                     /locus_tag="P5558_20775"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY26815.1"
                     /translation="SPPGACASFSTCMASRPEPGMTNPLRDLADGRLCCLNTATLGHR
                     EPILTVIDAVARAGFGAISPWRRDVEGHDAAAIGRRIKDAGLVLPGYCRSTYLPATTP
                     DGFRANVEDNRRAISDAASLGAQSFVMVVGGVAEGSKDLDHARRQVEDGTARLLEHGR
                     EVGVRIALEPLHPMYAGDRSCLNTLAQALDICDRLDPEPGPDPWLGVAIDCYHCWWDP
                     DRAAGIARAGAARRILGFHVCDWLVPTKDLLLDRGMMGDGVIDIPAIRGEIEAAGYQG
                     PAEVEVFSANDWWQRDREEVLKICRERFATVT"
     gene            951..1586
                     /locus_tag="P5558_20780"
     CDS             951..1586
                     /locus_tag="P5558_20780"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRY26816.1"
                     /translation="MRIDIVSDVVCPWCIIGYLQLAEALRTRKLEPEIHWHPFQLNPH
                     MPDEGQNLREHLAAKYGTTPEASVAARERLTRMGAELGFTFAYADDMRTYNTFRSHQL
                     LHWAATLGRQHELKMALFAAFFTRRENVGDPVVLADAAAGIGFDRALALAVLEDGRYA
                     EAVRQEMQFWVEQGIQGVPAIVLERRHLVSGAQGVERFGAILDQLTAPAIA"
     gene            complement(1595..3340)
                     /locus_tag="P5558_20785"
     CDS             complement(1595..3340)
                     /locus_tag="P5558_20785"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY26817.1"
                     /translation="MERRLTTILAADLVGYSRLMAADEEGVIARLRAARAEVVDPALA
                     EAGGRIVKTMGDGLLIEFTSPVAAVRMALLVQRAMAVRETGPEDERLRFRIGVNLGDI
                     VIDGDDILGDGVNVAARLESLAPAGGLCISRAVHDQVRGKLDAVLTPLGPQAVKNLPE
                     PVEVWRVEVEGVAVTGKVAAGMRPSIAVLPFDNMSRDPDQDFLADGIVEDVITELSRF
                     RDLTVIARNSSFSFRGTATDVRKIASELGVRYVVEGSVRRAGDRLRLTAQLIDAADGG
                     HVWADRWDRTMADLFDLQDELTRAIVTAVAPEISAHERTLARQKPTDSLTAWELTQRA
                     HAEYLSYTPASIEAAKALARRAIEADPGNVPAHTLLARVHATDAFSGRSQDAAASLRL
                     AHDAASRAIDLDSRSEAAFGAMGFVLAAEGRMHEALEALERARSLNPNNADVIQLGAY
                     VRLQPAIAEPRRALEDARLALRLSPADPFAWARRTLAGEALLQLGEYEAALAELEPAR
                     TAPNADWKPCIVAMVAALAAGRNSLAEDMLAEARGRRSDLTLAIVRRAQPWLFTLPAL
                     VPHAARLVELGLPQE"
     gene            3524..4864
                     /gene="gdhA"
                     /locus_tag="P5558_20790"
     CDS             3524..4864
                     /gene="gdhA"
                     /locus_tag="P5558_20790"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRY26818.1"
                     /translation="MDEKLEAIMAEVMRRNTGEEEFIQAVREVLESLGRVVAKRPDYT
                     DDALIERICEPERQIIFRVPWVDDRGHVCINRGFRVQFNSALGPYKGGLRFHPSVNLG
                     IVKFLGFEQTFKNALTGLPIGGGKGGSDFNPRGRSDGEIMRFCQSFMTELSRHLGEYT
                     DVPAGDIGVGGREIGYMFGQYKRLMNRYEAAVLTGKGLVYGGSRARKEATGYGAAYFV
                     EAMLATRGTSFDGKSCVVSGSGNVAIYTMEKIRANGGRTIACSDSNGYVVDERGIDLD
                     LVKELKEVRRERISVYAEEKGAGVRYISGGSIWDVPCDVAMPSATQNELTGKDARRLV
                     ENGVLAVGEGANMPSTPEAVRVFQEAGVLFAPGKAANAGGVATSALEMQQNASRDSWT
                     FEQTEQRLASIMRHIHDTCAATAEEFGAPGNYVLGANIAGFVRVAEAMRALGVI"
     gene            complement(4900..5673)
                     /locus_tag="P5558_20795"
     CDS             complement(4900..5673)
                     /locus_tag="P5558_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRY26819.1"
                     /translation="MALLSARDIHKKYDDTEVLKGIDLDIEQSEVVALIGASGSGKTT
                     FLRCINLLEEYHQGRIELEGESIGYTASGRRRKLGERQISAQRAKIGMVFQSFNLFPH
                     MTAEANVMLGLTKVRGMSNAEAADRARHWLDRVGLADRRRHYPYQLSGGQQQRVAIAR
                     AVAMEPRLMLFDEVTSALDPELVAEVLAVMQDLARSGMTMVVVSHEMLFVRDVATRVV
                     FMDGGLVAQSGTPDEVLHNPQTERLRSFLGRFHGIFGPV"
     gene            complement(5695..>5922)
                     /locus_tag="P5558_20800"
     CDS             complement(5695..>5922)
                     /locus_tag="P5558_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY26820.1"
                     /translation="ALPPWVNTALEIVKGTTLLSVIGVVELLLASQQAIARNYMILQF
                     YMAATIVYIIINFSIAQAAAALERRYAYLRY"
BASE COUNT          898 a         2142 c         1998 g          884 t
ORIGIN      
        1 ctcgccgccc ggcgcatgcg ccagcttctc gacctgcatg gcgtcgaggc ctgagccggg
       61 catgacgaac ccgctccgtg acctcgccga cggccggctc tgctgcctga acaccgccac
      121 tctcgggcac cgcgagccca tcctgacggt catcgacgcc gtggcccgcg ccggcttcgg
      181 cgccatcagc ccctggcgcc gcgacgtcga gggccatgac gcagccgcca tcggccgccg
      241 catcaaggat gccggcctcg tcctgcccgg ctactgccgc agcacctacc tgccggccac
      301 cacgccagat gggttccgcg ccaatgtcga ggacaaccgc cgggccatca gcgacgccgc
      361 ctcgctcggt gcccagagct tcgtcatggt ggtcggcggt gtcgccgaag gctcgaagga
      421 tctggaccac gcccgccggc aggtcgagga cggcacggcc cgcctcctcg agcatggccg
      481 cgaggtcggc gtcaggatcg ccctggagcc gctgcacccc atgtatgcgg gcgaccgctc
      541 ctgcctcaac accctggccc aggcgctgga catctgcgac cgcctcgacc cggagcccgg
      601 ccccgacccc tggctcggcg tcgccatcga ctgctaccac tgctggtggg accccgaccg
      661 cgccgccggc atcgcccgcg ccggcgctgc ccgccgcatc ctgggcttcc atgtctgcga
      721 ctggctggtg ccgaccaagg acctcctcct cgaccgcggc atgatgggcg acggcgtcat
      781 cgacatcccc gccatccgcg gcgagatcga ggccgccggc taccagggcc ccgccgaggt
      841 ggaggtcttc tccgccaacg actggtggca gcgcgaccgg gaggaggtcc tgaagatctg
      901 tcgcgagcgc ttcgcgaccg tcacctaagc gacaaccggg aggaatcccc atgcgcatcg
      961 atatcgtctc cgacgtcgtc tgcccctggt gcatcatcgg ctacctccag ctcgccgagg
     1021 cgctccgcac ccgcaagctc gagcccgaga tccactggca cccgttccag ctcaacccgc
     1081 acatgcccga cgaaggccag aacctgcgcg agcacctggc ggccaagtac ggcaccacgc
     1141 ccgaggcctc cgtggcggcc cgcgaacggc tgacgcgcat gggtgccgag ctcggcttca
     1201 ccttcgccta tgccgacgac atgcggacgt acaacacctt caggtcccac cagctcctgc
     1261 actgggccgc gaccctgggc cgccagcacg agctgaagat ggcgctgttt gccgccttct
     1321 tcacccgccg cgagaacgtg ggcgatccgg tcgtgctggc cgacgcggcg gccggcatcg
     1381 gcttcgaccg ggccctggcg ctggccgtgc tcgaggacgg ccgctacgcc gaggccgtgc
     1441 gccaggaaat gcagttctgg gtggagcagg gcatccaggg cgtgccggcc atcgtcctcg
     1501 agcgccgcca cctcgtctcc ggcgcccagg gcgtcgagcg cttcggcgcc atcctcgacc
     1561 agctcacggc gccggctatt gcctgaccgg cgggctattc ctgcggcagg cccagctcga
     1621 ccagccgtgc cgcgtgcggg accagtgccg gcagggtgaa gagccagggc tgggcgcgcc
     1681 ggacgatggc cagggtaagg tcggaacgcc gcccccgggc ttcggccagc atgtcttccg
     1741 caagcgagtt ccggccggct gcgagggcgg ccaccattgc aacgatgcag ggcttccagt
     1801 cggcattggg cgccgtgcgc gccggctcga gctcggccag tgccgcctcg tattcgccga
     1861 gctggagcag ggcctcgccc gcgagcgtgc ggcgcgccca ggcgaaggga tcggccgggc
     1921 tcagacgcag ggccaggcgc gcgtcctcga gggcccttct tggctcggcg atcgccggct
     1981 gcaggcggac ataggcgccc aattggatga cgtcggcatt gttcgggttc agcgagcgtg
     2041 cccgttccag ggcctcgagc gcctcgtgca tgcggccctc ggcggccagc acgaaaccca
     2101 tggcaccgaa ggccgcctcg ctccggctgt cgagatcgat ggcccgactg gcggcatcgt
     2161 gggcgaggcg caggctggcc gcggcatcct gggaacggcc cgagaaggcg tcagttgcgt
     2221 gcacccgggc gagcagggtg tgggccggca cgttgcccgg atcggcctcg atggcgcgcc
     2281 gggcgagcgc cttggccgct tcgatcgatg ccggcgtgta gctgagatat tcggcatgcg
     2341 cccgttgggt cagctcccag gcggtgaggc tgtccgtcgg cttctgccgc gccagtgtcc
     2401 gctcatgtgc actgatctcc ggtgccacgg ccgtcacgat cgcccgggtc agctcgtcct
     2461 gcaggtcgaa caggtcggcc atcgtccggt cccagcggtc ggcccagaca tggccgccgt
     2521 ccgcggcgtc gatgagctgg gccgtcagcc gcaggcggtc gccggcgcgg cggaccgagc
     2581 cctcgacgac atagcgcacg cccagctcgc tcgcgatctt ccgcacgtca gtggccgtgc
     2641 cacgaaagct gaagctggaa ttgcgcgcga tcaccgtgag gtcgcgaaag cgcgagagct
     2701 cggtgatcac gtcctcgacg atgccgtccg ccaggaagtc ctggtccggg tcacgcgaca
     2761 tgttgtcgaa gggcagcacg gcgatcgagg ggcgcatgcc ggctgccact ttcccggtga
     2821 ccgcgacgcc ctccacctcg acccgccaga cctcgaccgg ctccggcagg ttcttcaccg
     2881 cctgcgggcc gagcggtgtg agcaccgcat ccagcttgcc gcgcacctgg tcgtggacgg
     2941 cgcgcgagat gcagagcccg cccgcgggcg cgaggctttc gagccttgcc gcgacattga
     3001 ccccatctcc caggatgtcg tcgccgtcga tgacgatgtc gccgagattg acgccgatgc
     3061 gaaagcgcag ccgctcgtct tccggccccg tctcgcgcac cgccatcgcc cgctgcacaa
     3121 gcagcgccat ccgcaccgcc gccaccggcg aagtgaattc gatcagcagc ccgtcgccca
     3181 tcgtcttgac gatccgcccc ccggcctccg ccagcgccgg gtcgaccacc tccgcccgcg
     3241 ccgcccgcag ccgcgcgatg acgccctcct cgtcagccgc catcagccgc gagtagccga
     3301 ccagatcggc ggcgaggatg gtcgtcagcc ggcgttccac gcggcggtcc cttcatgaag
     3361 gtcgaggccg acggaaacta gccggcgagc gcgcgccgtc aaatcccgcc cgcgaaagcg
     3421 ctcgtcgcag ccttcattcc cgccgggttt cggtctattc gggtgccgtt cgcctgcggc
     3481 gcgggcgatc gcttgattcg atcggagaga ccccttgacg aaaatggacg agaagctcga
     3541 ggcgatcatg gccgaggtca tgcgccgcaa tacgggcgag gaggaattca tccaggccgt
     3601 ccgcgaggtg ctggaaagcc tcgggcgggt ggtggccaag cggccggact ataccgacga
     3661 cgcgctgatc gagcggatct gcgagcccga gcggcagatc atcttccgcg tgccctgggt
     3721 cgacgaccgg ggccatgtct gtatcaaccg cggctttcgc gtgcagttca actccgccct
     3781 cggcccctac aagggcggcc tgcgcttcca cccgtcggtc aatctcggca tcgtcaagtt
     3841 cctgggcttc gagcagacct tcaagaatgc cctgaccggc ctgcccatcg gcggcggcaa
     3901 gggcgggtcg gacttcaacc cgcgcggccg gtcggatggc gagatcatgc gcttctgcca
     3961 gtccttcatg accgagctgt cgcgccatct cggcgagtac accgacgtcc cggccggcga
     4021 tatcggcgtg ggcggccgcg agatcggcta catgttcggc cagtacaagc ggctcatgaa
     4081 tcgctacgag gcggccgtgc tcaccggcaa gggcctcgtc tatggcggct cgcgggcccg
     4141 caaggaggcg accggctacg gtgccgccta cttcgtcgag gccatgctgg ccaccagagg
     4201 cacctccttc gacggcaaga gctgcgttgt ctcgggctcg ggcaacgtcg ccatctacac
     4261 catggagaag atccgcgcga acggcggccg gaccatcgcc tgctccgaca gcaacggcta
     4321 cgtcgtcgac gagcgcggca tcgacctcga cctggtcaag gagctcaagg aggtccgccg
     4381 cgagcgcatc agcgtatacg ccgaggagaa gggcgcaggc gtccgctaca tcagcggcgg
     4441 ctcgatctgg gacgtgccct gcgacgtcgc catgccatcg gccacccaga acgagctgac
     4501 cggcaaggac gcccggcggc tggtcgagaa cggcgtgctg gcggtcggcg agggcgccaa
     4561 catgccctcc acccccgagg ccgtccgggt ctttcaggag gcgggcgtgc tgttcgcccc
     4621 cggcaaggcc gccaatgccg gcggcgtcgc cacctcggcg ctcgagatgc agcagaacgc
     4681 gtcccgcgac agctggacct tcgagcagac cgagcagcgc ctcgccagca tcatgcgcca
     4741 catccacgac acctgcgccg cgaccgccga ggagttcggc gcgccgggca actacgtcct
     4801 cggcgccaac atcgcgggct tcgtgcgggt ggccgaggcg atgcgggcgc tcggcgtgat
     4861 ctgagcgggt cccgggccgg ccgccgcccg ccatgctcct cagaccgggc cgaagatccc
     4921 gtggaaccgc ccgaggaagc tgcgcaggcg ctcggtctgg gggttgtgca ggacctcgtc
     4981 gggcgtgccg gactgggcga cgagtccgcc atccatgaag accacgcggg tcgccacgtc
     5041 gcgcacgaag agcatctcgt ggctgaccac caccatggtc atgccgctcc tcgccaggtc
     5101 ctgcatcacc gccagcacct cggcgaccag ctcggggtcg agcgccgagg tgacctcgtc
     5161 gaagagcata agccgcggct ccatcgccac cgcccgggcg atcgcgacgc gctgctgctg
     5221 gccgccggag agctggtagg gatagtgccg gcgacggtcg gcgaggccga cccggtcgag
     5281 ccagtgccgg gcgcggtccg ctgcctcggc gttcgacatg ccgcgcacct tggtcaggcc
     5341 gagcatgaca ttggcctcgg ccgtcatgtg cggaaagagg ttgaagctct ggaagaccat
     5401 gccgatcttc gcccgctgcg ccgagatctg ccgctcgccg agcttgcgcc gccggccgct
     5461 ggccgtgtag ccgatcgatt cgccctcgag ctcgatccgc ccctggtgat actcctcgag
     5521 caggttgatg cagcgcagga aggtggtctt gcccgagccc gaagcgccga tcagcgccac
     5581 cacctcgctc tgctcgatgt cgaggtcgat gcccttgagc acctcggtgt cgtcgtattt
     5641 cttgtggatg tcgcgggcgc tcaagagggc catcggtcgc tccgtcgcct ggactcaata
     5701 gcgcagatag gcatagcgcc gctccagcgc cgccgcggcc tgggcgatcg agaaattgat
     5761 gatgatgtag acgatggtcg ccgccatgta gaactgcagg atcatgtagt tgcgggcgat
     5821 cgcctgctgg ctggcgagca ggagctcgac cacgccgatc accgagagca aggtcgtgcc
     5881 cttgacgatc tcgagggcgg tgttgaccca gggcggcaag gc
//
LOCUS       DAOTFI010000274         5893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_16054_length_5893_cov_6.430798, whole genome shotgun sequence.
ACCESSION   DAOTFI010000274 DAOTFI010000000
VERSION     DAOTFI010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16054_length_5893_cov_6.430798"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /gene="glnA"
                     /locus_tag="P5558_20805"
     CDS             <1..215
                     /gene="glnA"
                     /locus_tag="P5558_20805"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRY26821.1"
                     /translation="PPEEAAEIPKVPGQLADAIAALEADHEYLLQGDVFTPDFIENYI
                     ALKRETELDPIRLRPHPYEFVMYYDC"
     gene            complement(308..1504)
                     /locus_tag="P5558_20810"
     CDS             complement(308..1504)
                     /locus_tag="P5558_20810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY26822.1"
                     /translation="MDGSAGVAPFAAAAMRRDAATWYGYLLLGFFTYLLNIQGNILPY
                     LRAELELGYRAAALHSSAIALGMILIGLQGERVIRRLGRARGLAFGSFGAALAAVGLC
                     LAPAAWASIAACFVLGFCGALIASVTPVMLADIHGRHRDRALAELGAACYAFAVMAPL
                     AMGASLALGFGWRGAVLMGVALGSLILLVGRSARIPESAVAADRRGRLPPLYWVYWVL
                     LALVVAVEFSVLLWAPAFLEIVVGLSPATAASTAALFALAMLVGRSAGSAVVGRIGAH
                     RLYAGALLVTLAGFALYWAGGGPLLAVPGLIVLGLGTALLYPLTLGLALEAAGPQGVA
                     ASARFMLAVGVAILTAPFLLGDLADRIGLEAAHLVIPALALLALGGLGLARLLERRLG
                     VDQPGG"
     gene            1674..2498
                     /locus_tag="P5558_20815"
     CDS             1674..2498
                     /locus_tag="P5558_20815"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRY26823.1"
                     /translation="MAEERWYYNDAGTSVGPFTRQRLLELARAHLILPSTPVWRNGMI
                     DWAPMAEAVHEGRSEGLDPPRLEPPVERAYPEPVEPDEAPAAISDERGWSTLPVAPWR
                     RYGARAIDTSINGLLGVMLLAYAWYSLAPRSADDFFSLVETPGGLLLDLVLTTFIAAI
                     VGGFVVGATGTSIGKLVFGVRVLDANLRPIGIRRGLAREFHIWWAGLALGIPLLVLFT
                     LLAAYRRLQRDQTTAWDRDTHLVLHRPRGTLQTVLNGVGILLVVLVNVLMRALAEG"
     gene            2568..3437
                     /locus_tag="P5558_20820"
     CDS             2568..3437
                     /locus_tag="P5558_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26824.1"
                     /translation="MPEERARSLTLLLTGLALLAGLSWLLNAHQPEIVAALRQTTGLL
                     VAMALHILALVVVMLLGLRLTVLLWRHPVRRRTGGAAGLLFLLSLCLVIYQAANAGAY
                     LLASRDLYAITLATDASPDATMRPLPSGNLLITGTLGPGLMRDFLAAEAAFGPFAAVE
                     ISSPGGLVVHALELARHVERHGLTVIVRDECLSACVPIALASPRAYAEKDAVFGFHRV
                     STPVELHSDMAGYTLEVTQIEMADFLRRHRVPESLLEEAARHGADTLHLVSARDMVEM
                     GLISGTLMGSPGS"
     gene            3514..4680
                     /locus_tag="P5558_20825"
     CDS             3514..4680
                     /locus_tag="P5558_20825"
                     /inference="COORDINATES: protein motif:HMM:NF004627.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRY26825.1"
                     /translation="MTDPTTAATLLAHDDLAAFDLGAGGPVVPPIFQTSLFTFESVAA
                     MEAVFAGERRQAIYSRGDNPTVMAFEAKLAALEGTAAARGFASGMAAISSTVLAFLEA
                     GDRMVAVRHCYPDAYRLFRRLLPRLGITVEFVDGRDLDAVARAVEGAKLLYLESPTSV
                     VFETHDVAAQAALARAAGAVSVIDNSWATPLFQQPHKSGVDLVLHSASKYISGHSDTV
                     AGVVCGARAHLDRINRLTYPFLGGKLAPFEAFLLLRGLRTLELRLKRQEASALQVARW
                     LRERPEVAKVNHPGLGNRQGLAGTSSLFSIELAENVDVTRFCDTLRLFRLGVSWGGHE
                     SLVFPAAVGLRQAGEHNSLVDFGVSPRLVRLHVGLEDPAELIADLAEGLGAAGQ"
     gene            complement(4708..5241)
                     /locus_tag="P5558_20830"
     CDS             complement(4708..5241)
                     /locus_tag="P5558_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRY26826.1"
                     /translation="MTELSTLDPHGVLVEPTTLRIQRLLPGPIERVWAFLTESDLRRK
                     WLAAGEMEMRVGAPFALTWRNDQLNDPPSARPEGFPEEHHMASRITELDPPRKLAFTW
                     GRSGSVAIELEPKGRDVLLTLIHRRLPDRDTLLKVSAGWHAHLDILVARASNKAPAPF
                     WDGWTRLKDEYDRRLPA"
     gene            complement(5238..5600)
                     /locus_tag="P5558_20835"
     CDS             complement(5238..5600)
                     /locus_tag="P5558_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY26827.1"
                     /translation="MVELHAAQLDAVFHALGDATRRRMLRELADGEERTVGQLAAPFA
                     ISLQAASKHLKVLEKAGMVRREVRGRTHHCRLDPGPLATAHEWLAFYERFWTDRLDVL
                     ERLLRAEDAQKTPKGDDR"
BASE COUNT          856 a         2132 c         2060 g          845 t
ORIGIN      
        1 tgccgcccga ggaagcggcc gagatcccga aggtcccggg ccagctcgcc gacgccatcg
       61 cggcgctcga ggccgaccac gagtacctgc tccagggtga cgtgttcacg cccgacttca
      121 tcgagaacta catcgccctg aagcgcgaga ccgagctcga cccgatccgg ctgcggccgc
      181 acccgtacga gttcgtcatg tactacgact gctgaagctc gcctgccggt gcccggatcg
      241 ggcgccggcg atctcctcgt cacgcaactt ccccttgcgg gcggctcccg ggccgcccgt
      301 cttcttttca gccgcccggc tggtcgaccc cgagccgccg ctccagcagc cgggcgaggc
      361 cgagcccgcc cagcgccagc agcgccaggg ccgggatgac gagatgcgcc gcctcgagcc
      421 cgatccggtc ggcgagatcg ccgagcagga agggggcggt gaggatcgcc accccgaccg
      481 ccagcatgaa ccgggcgctg gccgcgacac cctgcggccc ggccgcctcg agcgccaggc
      541 cgagcgtgag cggatagagc agcgccgtgc cgaggccgag gacgatgagc cccggcaccg
      601 ccagcagcgg cccgccgccg gcccagtaga gggcgaagcc ggcgagcgtc accagcaggg
      661 cgccggcata gaggcggtgc gccccgatcc gcccgaccac ggcgctgccg gcgctccgcc
      721 cgaccagcat ggcgagcgcg aagagggcgg cggtgctcgc cgccgtcgcg ggcgacaggc
      781 cgaccacgat ctcgaggaag gcgggcgccc agaggaggac actgaactcg acggcaacga
      841 cgagggcgag cagcacccag tagacccagt agagcggcgg caggcgcccg cgccggtcgg
      901 cggcgacagc gctctcggga atccgggccg agcggccgac cagcagaatc aggctgccga
      961 gcgccacgcc catgagcacg gcgccgcgcc agccgaagcc cagggcgagc gaggcgccca
     1021 tggcgagcgg cgccatgacg gcgaaggcgt agcaggcggc acccagctcg gcgagggcgc
     1081 ggtcgcgatg ccggccatgg atgtcggcga gcatgaccgg cgtcaccgag gcgatcaggg
     1141 caccgcagaa gccgaggacg aagcaggcgg cgatgctggc ccaggcggcc ggtgccaggc
     1201 acaggccgac cgccgcgagc gccgccccga agctgccgaa ggccagcccg cgcgcccggc
     1261 cgagccgccg gatcaccctc tcgccctgga gcccgatcag gatcatgccg agggcgatgg
     1321 ccgaggaatg cagggcggcc gcccggtagc cgagctcgag ctcggccctg agatacggca
     1381 ggatgttgcc ctggatgttg aggagatagg tgaagaagcc gagcagcagg tagccgtacc
     1441 acgtcgccgc gtcgcgccgc atggcggcag ccgcgaaggg cgccacgccc gccgatccgt
     1501 ccatgtgctc ctgatccttc gagagcgacg ccgggtcgcc gaggcctctc ggataggccg
     1561 gcgcccgtcg cggcgcaagt cacccatcgg tatggccgcc gtgctggaac cccgccggga
     1621 gttggcgtaa tccggcacca tcactcgtcg acacccgtgg gcaaggggcc gccgtggcgg
     1681 aagagcgctg gtactacaac gatgccggca cctcggtcgg gccgttcacc cggcaacggc
     1741 tgctggagct cgcgcgggcc cacctgatcc tgccctccac gccggtctgg cggaacggca
     1801 tgatcgactg ggcgcccatg gccgaggccg ttcacgaggg ccggtcggag ggcctcgacc
     1861 cgcctcgcct cgagccgccg gtcgagcggg cctaccccga gcccgtggag cccgacgagg
     1921 cgccggctgc catcagcgac gagcgcggct ggtcgaccct gccggtggcg ccctggcggc
     1981 gctacggcgc gcgcgccatc gacacgtcga tcaacggcct gctcggcgtc atgctgctgg
     2041 cctatgcctg gtactcgctc gccccccgct cagccgacga cttcttctcg ctcgtggaga
     2101 ccccgggcgg cctcctgctc gacctcgtgc tcaccacctt catcgcggcc atcgtcggcg
     2161 gcttcgtcgt cggcgccacg ggcacctcca tcggcaagct ggtcttcggc gtccgtgtcc
     2221 tggatgcgaa tctgcgaccc atcggcattc ggcgcggcct tgcccgggaa ttccacatct
     2281 ggtgggcggg actggcgctc ggcattccct tgctcgtcct cttcacgctc ctggccgcct
     2341 atcgcaggct gcagcgggac cagacgacgg cctgggaccg ggacacgcat ctcgtgctgc
     2401 accgcccgag gggcacgctg cagaccgtgc tcaacggcgt cggcatcctg ctcgtcgtcc
     2461 tggtcaacgt cctgatgcgg gccctggccg agggctgacg atgcgcattc ctgccgatcc
     2521 cgaggtgatc ggcagggggc tcgacgaaac tcacgaggag ccgatccatg cccgaagaac
     2581 gagcacgctc cctcaccctc ctcctcacgg gcctcgccct gctcgccggc ctttcctggc
     2641 tcctgaacgc gcaccagccg gagatcgtcg cggcgcttcg ccagacgacc ggcctcctgg
     2701 tggcgatggc gctgcacatt ctcgccctcg tggtcgtgat gctgctcggc ctgcggctga
     2761 ccgtcctgct ctggcggcat cccgtgcgcc ggcgcaccgg cggcgctgcc ggcctgctct
     2821 ttctgctctc cctttgcctc gtcatctatc aggccgccaa tgccggggcc tacctgctcg
     2881 cgtcgcgcga cctctatgcg atcacgctcg ccaccgacgc cagcccggac gcgacgatgc
     2941 ggccgctgcc ctcgggcaac ctgctcatca ccggcacgct cgggccgggc ctgatgcgcg
     3001 atttcctcgc cgccgaggca gccttcggcc ccttcgccgc cgtcgagatc tcgagcccgg
     3061 gcgggctcgt cgtgcatgcc ctcgagctcg cgcgccatgt cgagcggcac gggctgacgg
     3121 tcatcgtgcg cgacgaatgc ctgagcgcct gcgtgcccat tgccctggcc tcgccccgag
     3181 cctacgcgga gaaggatgcg gtcttcggct ttcatcgggt ctcgacaccg gtcgagctgc
     3241 actccgacat ggcgggctac acgctcgagg tcacccagat cgagatggcc gatttcctgc
     3301 gccgccatcg cgtccccgag agcctgctgg aggaggcggc gaggcacggg gccgacacgc
     3361 tgcatctggt ctccgcccgg gacatggtcg agatggggct catcagcggc acactgatgg
     3421 ggtcgccggg ctcgtgacgg cagcgttgtc ccgtggcagg ccaccgcccc gcctgctaag
     3481 cacgggcctt cccccggaga ggacccgccg cccgtgaccg accccaccac cgccgccacg
     3541 ctcctcgccc atgacgacct cgccgccttc gacctcggcg ccggcgggcc ggtggtgccg
     3601 ccgatcttcc agacctcgct cttcaccttc gagagcgtgg ccgccatgga ggcggtcttc
     3661 gccggcgagc gtcgccaggc gatctacagc cggggcgaca acccgacggt catggccttc
     3721 gaggcgaagc tggccgccct cgagggcacc gcggcggcgc gcggcttcgc ctccggcatg
     3781 gcggcgatca gcagcacggt cctggccttc ctcgaggcgg gcgaccgcat ggtggcggtg
     3841 cgccactgct acccggatgc gtaccggctc tttcgccgcc tcctgccgcg cctcggcatc
     3901 accgtcgaat tcgtcgacgg gcgggacctc gatgcggtgg cgcgggccgt cgagggggcg
     3961 aagctgctct acctggaaag cccgaccagc gtcgtcttcg agactcacga cgtggcggcc
     4021 caggcagcgc tggccagagc agccggtgcg gtcagcgtca tcgacaacag ctgggcgacg
     4081 ccgctcttcc agcagccgca caagtcaggc gtcgacctcg tcctgcattc ggcgagcaag
     4141 tacatctccg gccacagcga caccgtggcc ggcgtggtct gcggcgcccg tgcgcatctg
     4201 gaccgcatca accgtctcac ctaccccttc ctcggcggca agctggcccc gttcgaggcc
     4261 ttcctcctgc tgcgcggcct gcgcacgctg gaattgcggt tgaagcgcca ggaggcgagc
     4321 gcgctacagg tggcaagatg gctccgcgag cggccggagg tcgcgaaggt caaccacccg
     4381 gggctcggca accggcaggg cctcgccggg acctcgagcc tcttcagcat cgagctggcc
     4441 gagaacgtcg acgtcacccg cttctgcgac acactccgtc tctttcggct gggcgtgagc
     4501 tggggcggcc acgagagcct cgtctttccg gcggcggtgg ggctgcgcca ggccggcgag
     4561 cacaacagcc tcgtcgattt cggcgtctcg ccgcggctcg tccggctgca tgtcggcctc
     4621 gaggacccgg ccgagctgat cgccgacctg gcggaagggc tcggcgccgc cgggcagtag
     4681 ccggcggcgc cctgccctcg cgagacgtca ggccgggagg cggcggtcgt actcgtcctt
     4741 gaggcgggtc cagccatccc agaagggggc cggcgccttg ttcgaggcgc gggcgaccag
     4801 gatgtcgaga tgcgcgtgcc agccggcgct gaccttcagg agggtgtcgc ggtccggcag
     4861 gcggcgatgg attagggtga gcagcacgtc cctgcccttc ggctccagct cgatggcgac
     4921 gctgccgctc ctgccccagg tgaaggcgag cttgcgcggt ggatcgagct cggtgatccg
     4981 gctcgccatg tgatgctcct ccggaaagcc ttcgggccgc gcgctcggcg ggtcgttgag
     5041 ctggtcgttg cgccaggtga gcgcgaaggg tgcgcccacc cgcatctcca tctcgcccgc
     5101 ggccagccat ttgcggcgca ggtcgctctc ggtgaggaag gcccagaccc gctcgatcgg
     5161 gccgggcagg aggcgctgga tcctgagcgt ggtgggctcg accaggacgc catgggggtc
     5221 gagggtcgaa agctcggtca tcggtcgtct cccttcgggg ttttttgcgc atcctcggcg
     5281 cgcagcaggc gttcgagcac gtcgaggcga tcggtccaga agcgctcgta gaaggccagc
     5341 cactcgtggg cggtggcgag cgggccgggg tcgagccggc agtgatgggt gcggcctcgc
     5401 acctcgcgac gaaccatgcc ggccttctcc aggaccttga ggtgcttgga cgccgcctgg
     5461 agcgagatgg cgaagggtgc cgcgagctgg ccgaccgtcc gctcctcgcc atcggcgagc
     5521 tcgcgcagca tccgccgcct ggtggcatcg ccgagggcat ggaagacggc atcgagctgc
     5581 gctgcgtgaa gttcaaccat gtggttgagt atgagcaagg gccgacgaag agtcaaccaa
     5641 tcggttgaat gcatttcgtg gcggctggca ccgcaacccc gcgctggacc aaatgcggga
     5701 aatcgcgcac atctttcgcc aaccgatcgt gcaagggagg acttcatgcg caagctgaca
     5761 ttggcggcga cagtggcact tctgacgggt gccggcgggg cctcggccca gacgctgagc
     5821 ctggtcgagg tgatcacgag cccgcccagg accgagcaat tgcgggccat ggtcgaccag
     5881 ttcgaggcgg cca
//
LOCUS       DAOTFI010000275         5883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_16107_length_5883_cov_5.865477, whole genome shotgun sequence.
ACCESSION   DAOTFI010000275 DAOTFI010000000
VERSION     DAOTFI010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16107_length_5883_cov_5.865477"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5558_20840"
     CDS             <1..363
                     /locus_tag="P5558_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26828.1"
                     /translation="QRAMIAMMLIPDPDVLIADEPTSALDVTIQLQVLAIMDRLVEAR
                     GLGLILISHDLELVATFCDRVLVMYGGRIVEELDAARLGEARHPYTRGLLGCLPRPEK
                     RGSPLPVMVRDPAWLGAP"
     gene            360..1145
                     /locus_tag="P5558_20845"
     CDS             360..1145
                     /locus_tag="P5558_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY26829.1"
                     /translation="MIEVENLDVTFVTGKTRVAAVRGVSFSVAARSSFGLVGESGSGK
                     STILRVLSGLNPDWQGTIRIAGMALAKKPPLAFYKRVQMVFQDPFGSLHPRRTIDATL
                     REPLAIHRLANADARIARALEEVRLGPEFRYRFPHQLSGGQRQRVAIARALILEPELL
                     LLDEPTSALDVSIQAEIINLLNELRRRRGLTYLFVSHDLGVVAHLCDRLAIMRRGSIV
                     ESVAGDALAEGRLEQPYSRQLHLASQGYDRAVIDAFEASPEAT"
     gene            1291..2466
                     /locus_tag="P5558_20850"
     CDS             1291..2466
                     /locus_tag="P5558_20850"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRY26830.1"
                     /translation="MTDVVIVAAGRTAIGSFSGGLSSVPAHYLGQTAISGVLERAKVT
                     PAEVSEVILGQILTAGQGQNPARQASVNAGVPVEVPAYGVNILCGSGLKSVALGAQAI
                     KNGDSSVVVAGGQESMSQAPHVAHLRNGQKMGSLEFKDTMLRDGLMDAFQDYHMGTTA
                     ENIAQKWQITREDQDKFAVASQNKAEAAITAGRFKDEIIPVTIKTRKGDVTVDTDEFP
                     RFGATLEGMAKLRPAFSKDGTVTAGNASGINDGAAAVVLMSAEEAKKRGLKPLARIAS
                     WAQAGVDPAIMGTGPIPASKAALEKAGWTTDDLDLVEANEAFAAQAIAVNRELGWDTD
                     KVNVNGGAIALGHPIGASGARVLVTLLHEMQKRDAKKGLATLCIGGGMGIAMCLARD"
     gene            2618..3343
                     /locus_tag="P5558_20855"
     CDS             2618..3343
                     /locus_tag="P5558_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HRY26831.1"
                     /translation="MARVALVTGGSRGIGAAISVALKAAGYKVAANYAGNDEAAAKFK
                     AETGIPVFKWSVADYDACVAGIKQVEAELGPVEVLVNNAGITRDAMFHRMTPQQWNEV
                     VSTNLTGVFNMTHPIWPGMRERKFGRVIQISSVNGQKGQAGQSNYSAAKAGDIGFTRA
                     LAQEGARVGITVNAICPGYIGTEMVRAIDEKVLNERIIPQIPVGRLGEPEEIARCVVF
                     LASDEAGFLTGSTISPNGGQYMT"
     gene            complement(3378..3734)
                     /locus_tag="P5558_20860"
     CDS             complement(3378..3734)
                     /locus_tag="P5558_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26832.1"
                     /translation="MSGMPMDGAALLRLMDPRTALAAWRLWTQAAETGIHAAMTIQAR
                     LAMIATAMATGRDFPVTESWQMVAEKQQAMMESAAAAWRANPAFASDPRALLRTATAG
                     LRPYRRKTRSNARRLA"
     gene            4081..4338
                     /locus_tag="P5558_20865"
     CDS             4081..4338
                     /locus_tag="P5558_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26833.1"
                     /translation="MKLQILGGVAVAALLAVSGAKTSFAQAENCLEYMPVVQAKFDQA
                     SDEKKAKAKAHMDEALKAQSENNEQVCLEKLHMVDQELGGQ"
     gene            4989..5246
                     /locus_tag="P5558_20870"
     CDS             4989..5246
                     /locus_tag="P5558_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26834.1"
                     /translation="MNTKFLAAAALAAVFSVSAAETSFAQAENCLEYMPTVQAKFDGA
                     SAEAQAKAKDAMDAAIKAQSENNEEACLEHLHMADQQLGGQ"
     gene            complement(5668..>5883)
                     /locus_tag="P5558_20875"
     CDS             complement(5668..>5883)
                     /locus_tag="P5558_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26835.1"
                     /translation="GRFAVLLDDIGSGAATLIGRRLERLLTRVPHDLPITPRLTARSV
                     QLDDAMPPLRALHEAMRWRPQAPAAAA"
BASE COUNT          981 a         2075 c         1944 g          883 t
ORIGIN      
        1 cagcgggcga tgatcgccat gatgctgatc cccgatcccg acgtcctgat cgccgacgag
       61 ccgacctcgg ccctcgacgt caccatccag cttcaggtgc tggcgatcat ggaccggctg
      121 gtcgaggcgc gcggcctcgg cctcatcctg atcagccacg atctggagct ggtcgccacc
      181 ttctgcgacc gcgtgctcgt catgtatggc ggccgcatcg tcgaggagct cgatgccgcc
      241 cgcctcggcg aggcccggca cccctacacc cgcggtttgc tcggctgcct gccacgcccc
      301 gagaagcgcg gcagcccgtt gcccgtcatg gtccgcgatc cagcctggct gggtgcccct
      361 tgatcgaagt cgagaacctc gacgtcacct tcgtcaccgg caagacgcgg gtcgccgcgg
      421 tccggggcgt gagcttctcg gtcgccgcca gaagcagctt cgggctggtc ggcgagagcg
      481 gctcgggcaa gtccaccatc ctgcgcgtcc tctccggcct caaccccgac tggcagggga
      541 cgatccgcat cgccggcatg gcgctggcga aaaagccgcc gctcgccttc tacaagcgcg
      601 tgcagatggt cttccaggac cccttcggct cgctccaccc gagacgcacc atcgacgcca
      661 ccctgcgcga gcccttggcc atccatcgcc tggccaacgc cgatgcgcgg atcgcccgcg
      721 ccctcgagga ggtccggctc ggccccgagt tccgctaccg cttcccgcac cagctctccg
      781 gcggccagcg ccagcgcgtg gcgatcgccc gggccctgat cctcgagccc gagctcctgc
      841 tgctcgacga gccgacctcc gccctcgacg tctcgatcca ggccgagatc atcaacctcc
      901 tgaacgagct ccgccgccgg cgcggcctca cctatctttt cgtcagccac gatctcggcg
      961 tggtcgccca tctctgcgac cgactcgcca tcatgcgccg gggatccatc gtggaaagcg
     1021 tggcgggcga cgccctcgcc gaaggccggc tcgagcagcc ctacagccgc cagctccacc
     1081 tcgcgagcca gggctacgac cgggcggtca tcgacgcctt cgaggcatcg cccgaggcga
     1141 cgtgaaccat ggttgcggcg aatggctgca atgtcgggtt gtgggtcgcg ggcctgattg
     1201 caggctagta taaggcggcc acgcatcaaa ctgtacgctc tggcgctgga cgatccgggc
     1261 cgggcagcaa ggaatggggg aacgaccagc atgaccgatg tcgtcatcgt cgccgcgggc
     1321 aggaccgcga tcggcagctt cagcggcggg ctcagcagcg tgccggcgca ctatctcggc
     1381 cagaccgcga tcagtggcgt gctcgagcgc gccaaggtga cgcccgccga ggtctccgag
     1441 gtcattctcg gccagatcct caccgccggc cagggccaga acccggctcg ccaggcctcg
     1501 gtgaatgccg gcgtgccggt cgaggtgccc gcctatggcg tcaacatcct ctgcggctcc
     1561 ggcctgaagt cggtggccct cggcgcccag gcgatcaaga atggcgacag cagcgtcgtc
     1621 gtcgccggcg gccaggagag catgagccag gcgccgcacg tggcccatct caggaacggc
     1681 cagaagatgg gcagcctgga gttcaaggac accatgctgc gcgacggcct gatggacgcc
     1741 ttccaggatt accacatggg caccacggcc gagaacatcg cccagaagtg gcagatcacc
     1801 cgggaagacc aggacaagtt cgcggtcgcc tcgcagaaca aggccgaggc ggcgatcact
     1861 gccggccggt tcaaggacga gatcatcccg gtcaccatca agaccaggaa gggcgacgtc
     1921 accgtcgaca ccgacgagtt cccgcgtttc ggcgccacgc tcgaaggcat ggcgaagctg
     1981 cgcccggcct tcagcaagga cggcacggtg accgccggca atgcgtccgg catcaatgac
     2041 ggggccgccg ccgtcgtcct catgagcgcc gaggaagcga agaagcgtgg cctgaagccg
     2101 ctcgcccgga tcgcgtcctg ggcgcaggcc ggcgtcgatc cggcgatcat gggcaccggc
     2161 cccatcccgg cctcgaaggc ggcgctggag aaggccggct ggaccaccga cgacctcgac
     2221 ctcgtcgagg ccaacgaggc cttcgccgcc caggccatcg ccgtcaatcg cgagctcggc
     2281 tgggacaccg acaaggtcaa cgtcaatggc ggcgccattg cgctcggcca tcccatcggt
     2341 gcctccggtg cccgggtgct cgtcaccctg ctccacgaga tgcagaagcg cgacgccaag
     2401 aaaggcctcg ccacgctctg catcggcggc ggcatgggca tcgccatgtg cctcgcccgc
     2461 gactgagccg gatcacgtca atcggagccg ggcggtggcg aagagctgcc gcccggcttg
     2521 acattccggc gtcttgaacg aggctgaagg aagaccgacg aggtcgggca ccgacccggc
     2581 gacgatcagg ccgaaaacga tcaggggaga agcgacaatg gcacgagttg cattggtgac
     2641 gggcggcagc cgcggcatag gtgccgcgat ctcggtggcg ctgaaggccg ccggctacaa
     2701 ggtcgcggcc aactacgccg gcaacgacga ggcggcggcg aagttcaagg ccgagaccgg
     2761 cattcccgtc ttcaagtgga gcgtcgccga ctacgacgcc tgcgtcgccg gcatcaagca
     2821 ggtcgaggcc gagctcggcc ccgtcgaggt gctggtcaac aatgccggca tcacccgcga
     2881 cgccatgttc caccgcatga cgccgcagca gtggaacgag gtcgtctcga ccaacctcac
     2941 cggcgtcttc aacatgaccc acccgatctg gcccggcatg cgcgagcgca agttcggccg
     3001 ggtcatccag atcagctcgg tcaacggcca gaagggccag gccggccagt ccaactactc
     3061 ggcagccaag gccggcgaca tcggcttcac ccgcgccctg gcgcaggaag gtgcccgggt
     3121 cggcatcacc gtcaacgcca tctgcccggg ctacattggc accgagatgg tccgcgccat
     3181 cgacgagaag gtgctgaacg agcgcatcat cccgcagatc cccgtcggcc gcctcggcga
     3241 gcccgaggag atcgcccgct gcgtcgtctt cctcgccagc gacgaagccg gcttcctcac
     3301 cggctcgacc atctcgccga acggcggcca gtacatgacc tgagcgcggg ccggcgcctc
     3361 gcgatcggcg gctggcgcta cgccagccgc cgcgcattgc tccgggtctt ccgccgatag
     3421 ggccgcagtc cggccgtggc cgtgcgcagg agggccctag gatcgctggc gaaggccggg
     3481 ttggcgcgcc acgcggccgc ggccgattcc atcatcgcct gctgcttctc ggcgaccatc
     3541 tgccagctct cggtgaccgg gaagtcgcgg cccgtcgcca tggccgtggc gatcatggcg
     3601 agtcgtgcct ggatcgtcat ggcagcgtgg atcccggtct cggccgcctg cgtccagagc
     3661 cgccaggcgg cgagcgccgt cctcgggtcc atgagccgca agagggccgc tccgtccatc
     3721 ggcatgcctg acatcgattt ctcctggtga gtcgcccgga caatagcggc aaaggcccac
     3781 gcgcgcacga tcgcccgctc cttcgactcc gacccggctc agcctaaccg tgagccctct
     3841 gcagggttcc gaccccggtc ccgtatactg gccggcctgg tcgacttcca cgtcgacaac
     3901 ctcccgcagc gaacgctgga gaagcgacgc gctgccaacg caacttactg taaatttcgg
     3961 ggcgtagccc gaaagcacta gagtgtgtcg gtcggatcgg ccagtattgg tcgtggcccg
     4021 ggcggctgcc gaaggattcg gctgcactcg attggccgga ccaagtgaag ggagtccccg
     4081 atgaagcttc agatcctcgg tggtgtcgcg gttgcggcgc tgctcgccgt ctccggtgcc
     4141 aagacgagct ttgcccaggc cgagaactgc ctggagtaca tgccggtcgt ccaggcgaag
     4201 ttcgatcagg cttcggacga gaagaaggcc aaggcgaagg cgcacatgga cgaggccctc
     4261 aaggcccagt ccgagaacaa cgagcaggtc tgcctcgaga agctgcacat ggtcgaccag
     4321 gagctcggcg gccagtaagc cgaacgttgc agatagcggc tgatccaggc ggtcggcagc
     4381 cccgaccggg gcgctcggat ccttgaacca ggggatgcgc cgccagatca gacgaagttc
     4441 cggacaccgg tgaccgccgc tcggccaccg gtgtcacgtt ttttggtgcg acaatggcgt
     4501 cgcccgccac gatcgcctgg gcgggttatc gtgccacgct ccatgactgg cctcgctgac
     4561 ctgccgttgc cgaccttcgg caggcatccg tggcgcaagc cgcgccgcag ccccgagccg
     4621 gatcggtgac gctcgacagg acggcggcag tggcccgctc tcgcaggagc cgggttctgc
     4681 ggccggtcca cggggtcgag atcgccattt tgccgacgcc gatcatcggg ccgtccggcc
     4741 cgtgctgctc gccggccatg tccaggctgc ggccgacggg gcgcccttcg gggcggcgac
     4801 aggcatccga tccaccgcgt gatcacggcg accgttcctc cttgcgcgcc ggatctgcgg
     4861 aaaaaatgct tgccgtagaa cgaaatggct tgaatagcgc gcgcatgccg gcaagactgc
     4921 gtcgagcgaa ctcgccgggg cgccggcagg tcctgcgatc gtcgttttca acgtatggga
     4981 gtaacgccat gaacacgaaa ttccttgctg cggcagccct cgccgcggtc ttctccgttt
     5041 cggccgccga gaccagcttc gcccaggccg agaactgcct ggagtacatg ccgaccgtcc
     5101 aggccaagtt cgacggggcg tcggccgagg cgcaggccaa ggcgaaggac gccatggacg
     5161 ccgccatcaa ggcgcagtcc gagaacaacg aggaagcctg cctcgagcat ctgcacatgg
     5221 ccgaccagca gctcggcggc cagtaaggct cgaccagctt cggcggtcgg atcacagcca
     5281 gtcggtctcg tgccgacggg aatttcccgg tctgtcgcac aggtgcggca gagcgggtgt
     5341 gccgatctgc caactggatg aatcgcccat gcctccggca tgggcgattc gcgtttcatg
     5401 gcgaccccgg tcgccgtcgg accactgcct ctggcctcgc tccgatcggc gatctcggcc
     5461 gccttcgccg tgcggctggg ccggatccgc ggctgacccc ggctgtgccg aatcgttcag
     5521 ggccaatcca ttcgggccat tcgggttggc ctttcgctga tcgctccaat ccgggaacgc
     5581 caccagactc actggcacgg cgctggtgaa gcatgcggca ctgtcacatg cgcaacggcc
     5641 gcccgcccca cccgctgtca gggcttctca ggctgctgca gccggcgcct gggggcgcca
     5701 gcgcatcgcc tcgtgcagcg ctcgcaaggg cggcatggcg tcatcgagct ggaccgagcg
     5761 ggccgtcagc cggggtgtga tcggcaggtc gtgcggcacc cgtgtcagca accgctcgag
     5821 ccggcggccg atcagcgtgg cggctcccga gccgatgtcg tcgagcagga cggcgaaacg
     5881 tcc
//
LOCUS       DAOTFI010000276         5795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_16399_length_5795_cov_6.190592, whole genome shotgun sequence.
ACCESSION   DAOTFI010000276 DAOTFI010000000
VERSION     DAOTFI010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16399_length_5795_cov_6.190592"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="P5558_20880"
     CDS             <1..727
                     /locus_tag="P5558_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose synthase"
                     /protein_id="HRY26836.1"
                     /translation="DRDTMPTVEEQTATYTEVFEAFGPKPDMQLFGRGLRRRLPTMVD
                     GDQDRIRMIYSLAFALPGAPVLFYGEEIGMAENLDIPGRMSVRAPMQWAAGPNGGFSS
                     APVGAMRRPVVPGRKWGPAGINVQDQERDPESLLRWIERLIRRRRQTPEIAFGDWRVI
                     DTPEAAVIALRYDWGRCSAIVLHNLADEPVETAFELDKEDRADRLACLLGKGDVSARK
                     GGRVVSELPRYGYQWLRVARDGG"
     gene            complement(724..1269)
                     /locus_tag="P5558_20885"
     CDS             complement(724..1269)
                     /locus_tag="P5558_20885"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRY26837.1"
                     /translation="MRRNTEERWGSVSIGLHWTVAGLVLLVQVPAGIAMLRVDPGMLQ
                     DTLFTLHKNVGLIVLALALFRLGWRLANPVPALPDDLPGLQARLARLTHVLLYALLFL
                     LPISGFLFTAFGGFPVPLLLLVDIGKLVPASEPQAEVWKAIHHWAQWALYLVVTLHVL
                     GALQHALIRRDGVFQRMLGRR"
     gene            complement(1357..2730)
                     /locus_tag="P5558_20890"
     CDS             complement(1357..2730)
                     /locus_tag="P5558_20890"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HRY26838.1"
                     /translation="MRSMLKLAVLGALTAGPALAGTITGTATYRERIALPPEAVLEAT
                     LEDVSRADAPAVVLGRARVEPAGQVPIPFTITYDDRAIDERMSYAVRGRIIVGDELRF
                     TTTESHPVLTRGGGSEVTLRLQRVAGDAAAVPEPGGPLDPLPASFTGTLPCADCPGID
                     WQVDLLADGSFQLRQTYQDREPANAHDDIGRWAIGSDRRSLILQGGREAPVLLEIRNA
                     DTLRLMDRDGRPIESDLPYDLARADVHPLEPRLTMRGMYSYLADAGSFTECLTGRRLP
                     VAMVADNAALEQAYLEARSEPGAPLLVIVEGRIALLPPMEGPNPVPQLEPLEFIRVEP
                     EAACPAPFTQATLEGTDWRLTRLGDEAVLPADWRDAPGLGFDAATGRFAGSGGCNRLM
                     GGYTLDGDRIAFGAVAGTMMFCDGLMETEQAMTAALGAAHGWRVLGRQLELYDAEGDL
                     LARFAAD"
     gene            complement(2750..4756)
                     /locus_tag="P5558_20895"
     CDS             complement(2750..4756)
                     /locus_tag="P5558_20895"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY26839.1"
                     /translation="MDRRAGATASADPPPTPKEALLRQLQGQQRSLALINATVALVFT
                     GFAVLYVPQGPILAWLAFLLAAQALRLVAAGDGAMLGLRLTLVSLLTGLGWGAMGWLF
                     GTLDLVIVDAVVAFVLAGMAAGSITALPAHPPAFSTFLLATLLPFAVKLGLDPRPHTD
                     VMALLTVLYFIGIAYLGHHTWRTQTANAGLFLRNQALVNSLLLAGQQQEARVIERTRQ
                     LAEANAQMAAEIRRRRRTEAQVRHLLHHDPLTNLPNRLVFADRLETALRRAQRGRSMV
                     AVTLLDIDRFKAINDTLGHVTADLLLRALATRLASACRTSDTVARMGGDEFAAIFPDI
                     KVPADAEALAGKLVEHLKEPFRIDGAEIAISASFGVALYPDHGRDAATLLTGADLALY
                     DAKQKGRGQYRLLSAEMLELARGRRQIEGELEGAVERGELRILYQPQTSLSRNRITGA
                     EALVRWLHPVHGLLAPGAFIPAAESSGLIREIDHWVLANACRAATAWQTFGHPVRVAV
                     NLSPIEFRQPGLAGRIAAHLQNAGLAPDLLEIEITESAYLDRETASVEEQLGQIKDLG
                     VRIAIDDFGTGYASLAYLRWLPIDVIKIDRSFVSGIATSRHDAAIVASTVSLADTLGK
                     TVIAEGVETEQQAGILEQLGCDELQGYLLGRPASVRQLRRRLAA"
     gene            4950..>5795
                     /locus_tag="P5558_20900"
     CDS             4950..>5795
                     /locus_tag="P5558_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HRY26840.1"
                     /translation="MRIGVPREQQPGEQRVAASPETVKKLVAAGVEVVIEAGAGRGAA
                     IADEAYKSAGATVADADAAWAADLVLKVQRPTEAEQDRLREGQILVGALDPYQSREQV
                     AAYAAKGVTAFALELLPRTTRAQAMDILSSQANLAGYKIVVDAVAAYSRCMPMMMTAA
                     GTVTPARFFVVGAGVAGLQAIATARRMGAVVTATDVRPAAKQEIESLGAKFVGFIPEG
                     GATSGGYARPLTPEEQQQQAQMVAEHLKSQDIVVTTAQIPGRKAPRIVTAAMVESMKA
                     GSVVMD"
BASE COUNT          869 a         1851 c         2284 g          791 t
ORIGIN      
        1 cgaccgcgac acgatgccga ccgtggagga gcagaccgcc acctacaccg aggtgttcga
       61 ggccttcggc cccaagccgg acatgcagct cttcggccgc gggctccgcc gccgcctgcc
      121 caccatggtg gacggcgacc aggaccggat ccgcatgatc tacagcctcg ccttcgcgct
      181 gcccggcgca ccggtgctgt tctacggcga ggagatcggc atggccgaga acctggacat
      241 tcccgggcgc atgagcgtgc gcgcgccgat gcaatgggcc gcggggccca acggcggctt
      301 ctcgagcgcg ccggtggggg ccatgcgccg gccggtcgtc ccgggccgca agtggggccc
      361 ggccgggatc aatgtgcagg atcaggagcg cgatcccgag tcgctgctgc gctggatcga
      421 gcgcctgatc cgacggcggc gccagacgcc cgagatcgcg ttcggcgact ggcgcgtcat
      481 cgacacgccg gaggcggcgg tgatcgccct gcgctacgat tggggccggt gctccgcgat
      541 cgtcctgcac aatctggccg acgagcccgt cgagacggcg ttcgagctgg acaaggagga
      601 ccgggcggac cggctggcat gtctgctcgg caagggcgat gtcagtgcgc ggaagggcgg
      661 cagggtcgtg agcgagctgc cgcgctacgg ctatcaatgg ctgcgggtcg ccagggatgg
      721 cggctagcgg cggccgagca tcctctggaa aacgccgtcg cggcggatga gcgcgtgctg
      781 cagggcgccc agcacatgca gggtcacgac gagatagagc gcccattgcg cccagtggtg
      841 gatggctttc cacacctccg cctggggctc gctggcgggc acgagcttgc cgatatcgac
      901 cagcaggagg agcggcaccg gaaagccgcc gaaggccgtg aagagaaagc cgctgatcgg
      961 caggaggaag agcagggcgt agagcaggac gtgggtcagg cgggcgaggc gcgcctgcag
     1021 gccgggcagg tcgtccggca aggccgggac cgggttggcg aggcgccagc cgaggcggaa
     1081 gagggcgagg gcgaggacga tcaggccgac attcttgtgc agggtgaaga gggtgtcctg
     1141 cagcatgccc ggatccaccc gcagcatggc gatgccggcc gggacctgga ccaggaggac
     1201 gaggccggcg acggtccagt gcaggccgat gctgacgctg ccccagcgtt cctcggtgtt
     1261 gcggcgcatg gcccctccgc gtgctgccgt gccatgcccg tggacatggg gcgggatgcg
     1321 gcttcgggcg aggggtcgtg ccgcggcggc ccgcggtcag tcggcggcga agcgggcgag
     1381 gaggtcgccc tcggcgtcgt agagctcgag ctggcggccg agcacgcgcc agccgtgtgc
     1441 cgcaccgagg gcggcggtca tggcctgctc cgtctccatc agtccatcgc agaacatcat
     1501 cgtgccggcg acagcgccaa aggcgatcct gtcgccatcg agcgtgtagc cgcccatgag
     1561 gcggttgcag ccgccggacc cggcgaagcg cccggtcgcc gcgtcgaagc cgagcccggg
     1621 cgcatcgcgc cagtcggccg gcaggacggc ctcgtcgccg agccgggtca gccgccagtc
     1681 ggtgccttcg agggtcgcct gggtgaaggg cgcggggcag gccgcctcgg gctcgacacg
     1741 gatgaactcg aggggctcga gctgcggcac gggattcggc ccttccatcg gcggcaggag
     1801 ggcgatgcgg ccctcgacga tgacgaggag cggggcaccg ggctcgctgc gggcttcgag
     1861 atatgcctgc tcgagggcgg cgttgtcggc caccatggcg accggcaggc ggcggccggt
     1921 caggcattcg gtgaagctgc cggcgtcggc caggtagctg tacatgccgc gcatcgtgag
     1981 cctcggctcg agcgggtgaa cgtcggcgcg ggcgagatcg tagggcagat cggactcgat
     2041 cggccggccg tcccggtcca tcaggcggag cgtgtcggca ttgcggatct cgaggagcac
     2101 cggcgcttcc cggccgccct ggaggatcag gctcctccga tcgctgccga tggcccagcg
     2161 gccgatgtcg tcatgggcgt tcgccggctc gcgatcctgg tacgtctggc ggagctggaa
     2221 gctgccgtcg gcgaggaggt cgacctgcca gtcgatgccg gggcagtcgg cgcagggcag
     2281 ggtgcccgtg aagctcgcgg gcagggggtc gagcgggccg cccggctcag ggacggccgc
     2341 cgcatcgccg gcaacgcgct gcaggcggag cgtcacctcg ctgccgccgc cccgggtcag
     2401 caccggatgg gactcggtcg tggtgaaccg gagctcgtcg ccgacgatga tccggccccg
     2461 caccgcgtag ctcatgcgct cgtcgatcgc cctgtcgtcg taggtgatgg tgaaggggat
     2521 cggcacctgg ccggccggct cgacgcgggc ccggcccagc acgacggcgg gcgcgtcggc
     2581 gcgggagacg tcctcgaggg tcgcctcgag gacggcttcg ggcggcaggg cgatccgttc
     2641 gcggtaggtg gccgtgccgg tgatcgtgcc ggccagggcc gggccggccg tcagggcgcc
     2701 gagaacggcg agcttcagca tgctgcgcat gagacgattc ttcctttcat caggcggcga
     2761 gccggcggcg gagctggcgg acgcttgccg ggcgaccgag gagatagccc tgcagctcgt
     2821 cgcaccccag ctgctcgagg atgccggcct gctgctccgt ttccacgcct tcggcgatga
     2881 cggtcttgcc cagcgtatcg gcgagcgaga cggtgctggc gacgatggcg gcgtcgtgcc
     2941 ggctcgtggc gatgccggag acgaagctgc ggtcgatctt gatgacgtcg atcggcagcc
     3001 agcgcagata ggcgagcgac gcgtagccgg tgccgaaatc gtcgatggcg atgcgcacgc
     3061 cgagatcctt gatctggccg agctgctcct cgaccgaggc ggtctcgcgg tcgagatagg
     3121 cactttcggt gatctcgatc tcgagcaggt cgggggcgag gccggcattc tggagatgcg
     3181 ccgcgatgcg gccggcgagg ccgggctggc ggaactcgat cggcgagagg ttgaccgcga
     3241 cccggaccgg gtggccgaag gtctgccagg ccgtggccgc ccggcaggca ttggccagga
     3301 cccagtggtc gatctcgcgg atgaggccgg aggattcggc cgccgggatg aaggcgccgg
     3361 gggcgaggag gccgtggacg gggtggagcc agcggaccag cgcctcggcc ccggtgatgc
     3421 ggttgcggct gagcgaggtc tgcggctggt agaggatcct gagctcgccg cgctcgacgg
     3481 caccctcgag ctcgccctcg atctgccggc ggccgcgggc gagctccagc atctcggccg
     3541 aaagcagccg gtactggcct cttcccttct gcttggcgtc gtagagagcg aggtcggcgc
     3601 ccgtgagcag ggtcgccgca tcccggccgt ggtcgggata gagcgccacg ccgaagctcg
     3661 ccgagatggc gatctccgca ccgtcgatcc ggaaaggctc cttgagatgc tcgaccagct
     3721 tgccggcaag cgcctcggca tcggccggaa ccttgatgtc cgggaagatg gccgcgaact
     3781 cgtcgccgcc catccgggcg acggtgtcgc tcgtccggca ggcgctggcg aggcgggtcg
     3841 cgagcgcgcg cagcaggagg tcggccgtga cgtggccgag cgtgtcgttg atcgccttga
     3901 agcggtcgat gtcgaggagg gtgacggcga ccatcgagcg gccgcgctgg gcgcggcgca
     3961 gcgccgtctc caggcggtcg gcgaagacca ggcggttcgg caggttggtc aggggatcgt
     4021 ggtggaggag gtggcggacc tgcgcctcgg tgcgtcgtct tctcctgatc tcggccgcca
     4081 tctgggcgtt cgcctcggcg agctgccgcg tgcgctcgat gacccgcgcc tcctgctgct
     4141 ggccggccag cagcagggaa ttgaccagcg cctggttgcg caggaagagg ccggcattgg
     4201 cggtctgggt gcgccaggtg tggtggccga gataggcgat gccgatgaag tagagcacgg
     4261 tcaggagcgc catgacgtcg gtgtgcgggc gggggtcgag gccgagcttg acggcgaacg
     4321 gcaggagggt cgccaaaagg aacgtgctga aggccggcgg gtgggccggc agggcggtga
     4381 tggagccggc ggccatgccg gccaggacga aggccacgac cgcatcgacg atcaccaggt
     4441 cgagcgtgcc gaagagccag cccatggccc cccagccgag gccggtgagc agcgagacca
     4501 gggtcagcct gagaccgagc atggcgccgt cgccggcggc cacgaggcgc aacgcctggg
     4561 ccgcgaggag gaaggcgagc caggcgagga tgggaccctg cgggacgtag agcacggcga
     4621 agccggtgaa gaccagggcg accgtggcgt tgatgagagc gagcgagcgc tgctggccct
     4681 gcagttgccg gagcagcgcc tccttcggtg tgggcggcgg gtcggcagag gcggtggcgc
     4741 cagcgcgtcg atccaaggcg tcgttcctcg tgcggcggcc gacggcccac cgaagactcg
     4801 gctcgttgcc gctgaaagta gagagttccg cgtggacact caatcatgac gcaaacgagc
     4861 cgcgtgcctg accgcgaagg ttgcattgcg ccggtcccgg cgattgccta tcaacgcggg
     4921 cgcaaggttg agggaggtgg cggccagcca tgaggatcgg agtccccagg gagcagcagc
     4981 cgggcgagca gcgcgtcgcg gcctcgcccg agacggtgaa gaagctcgtc gccgccggtg
     5041 tcgaggtggt gatcgaggcg ggtgccggcc gcggtgcggc gatcgccgac gaggcataca
     5101 agagcgccgg ggcgacggtc gccgatgccg acgccgcctg ggcggccgat ctggtgctca
     5161 aggtgcagcg gccgaccgag gccgagcagg accggctgcg cgaggggcag atcctggtgg
     5221 gtgccctcga tccctatcag agccgggagc aggtcgcggc ctatgcggcg aagggggtga
     5281 ccgccttcgc gctggagctg ctgccgcgca ccacccgcgc ccaggccatg gacatcctct
     5341 cgagccaggc caatctcgcc ggctacaaga tcgtggtcga cgcggtcgcc gcctactcgc
     5401 gctgcatgcc gatgatgatg accgccgccg gcacggtgac gccggccagg ttcttcgtcg
     5461 tcggcgccgg cgtcgccggg ctgcaggcga tcgccactgc ccggcgcatg ggggccgtgg
     5521 tgaccgccac cgacgtccgc ccggcggcga aacaggagat cgagagcctg ggggcgaagt
     5581 tcgtgggctt catccccgag ggcggggcga cctcgggcgg ctatgccagg ccgctgacgc
     5641 cggaggagca gcagcagcag gcccagatgg tggccgagca tctcaagagc caggacatcg
     5701 tcgtgaccac ggcgcagatc ccgggccgca aggcaccccg gatcgtcacc gccgccatgg
     5761 tggaatcgat gaaggcgggc tcggtggtca tggat
//
LOCUS       DAOTFI010000277         5755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_16555_length_5755_cov_6.951228, whole genome shotgun sequence.
ACCESSION   DAOTFI010000277 DAOTFI010000000
VERSION     DAOTFI010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16555_length_5755_cov_6.951228"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="P5558_20905"
     CDS             complement(<1..212)
                     /locus_tag="P5558_20905"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRY26841.1"
                     /translation="MLLLFILVPLAEVGVFIQVGGLIGLWPTLALTVLTAIIGSICIR
                     WQGLRLVGQARAQLQAGEAPVFEVVSG"
     gene            354..1046
                     /locus_tag="P5558_20910"
     CDS             354..1046
                     /locus_tag="P5558_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HRY26842.1"
                     /translation="MSESFAFIDIIFFAMVAAFIAFRLRSVLGRRTGNERRRANPVLT
                     PDRAAEHQSNDNVVPLPDRNASQQAEEPNFADIGDPAIKQGVTEIRLADHKFDLDGFL
                     QGAKAAFGIIIEAFANGDKAALRPLLADDVYASFAAAIGAREAAGERLSTEIVSIRSA
                     DIAAAGMSGPYARLTIRFVTEQINVTHDATDNVVDGDPTRIDEVVDIWTFSRDTRTSD
                     PNWQLIETRTPD"
     gene            1057..2265
                     /locus_tag="P5558_20915"
     CDS             1057..2265
                     /locus_tag="P5558_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRY26843.1"
                     /translation="MIDRSPRPGGPWAGTLVAGLFLLLLTACGKPPEAPPMALEPVAF
                     TALAGWADDRQDQALSSFLRSCAPLARRDPEAPFHKAEPAAGRNADWQALCRQAAATP
                     VEPAAARAFFETGFQPYRVLGDGESEGLFTGYFEPLLHGAARPNGTASIPLRAAPGDI
                     VTVDLGQFADDLDGRKVRGRVTGDRLEPYPSRGEIERGALDGRGLELLWVDDPIEKFF
                     LQIQGSGLVELDDGRTLRVGYADQNGRAYRAIGRDLIELGELTKEEVSLQTIEAWLRA
                     HPDRAREIMDRNASYVFFRVLGEAGALEGPEGAQGVPLQAERSLAVDRSFIPLGAPLW
                     LETEAPFPDGERPFRRLVVAQDTGGAIKGAVRGDVFWGAGDLAAFVAGHMKSRGSYYL
                     LLPRTARPVS"
     gene            2294..3094
                     /locus_tag="P5558_20920"
     CDS             2294..3094
                     /locus_tag="P5558_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRY26844.1"
                     /translation="MRPAPSAAAPDRPRVALFVTCLVDLFRPSIGFAAIRLLEAAGCT
                     VEVPKAQTCCGQPAFNSGDRATAEAIARQVLDTFEPYDYVVAPSGSCAGMLRRHLPEL
                     FDTDPVARERASVLAERCHELLGFLVDIRGLKEVASHFAGTVTYHDSCSGLRELGVKA
                     QPRQLLLGVPGVDLVEMREPEVCCGFGGTFCVKYPEISTRMVSDKTADIAATGADTLA
                     AGDLGCLMNMAGRLQRQGSKVRAYHVAEILAGPGAGTATLPGIGERRR"
     gene            3094..4515
                     /locus_tag="P5558_20925"
     CDS             3094..4515
                     /locus_tag="P5558_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRY26845.1"
                     /translation="MQPTSLAFEAKAHAALDNPALQSALGSIKTGWQAGRSRAAEALP
                     EFEALRDRGRDIKNHTLQHLDLYLEMFETKLAAAGGQLHFARDAAEARATVLDLCRTA
                     EARAVTKSKSMVAEEIGINEHLEANGIERIETDLGEYIIQLRKEPPSHLVGPAVHLTK
                     DQVSDLFAEHHGGPRIEDPAALVAEARRVLREKYFQADIGITGANFLVAETGTIVTVT
                     NEGNAELTQGLPRTHIVITSLEKLVPTIDDAFTLLRLLARSATGQEFSAYTTLVTGPK
                     RPADLDGPDRLHVVLVDNGRTKMLGTALQEMLRCIRCGACMNHCPVYLAAGGHAYGWV
                     YVGPMGSVLTPQFAGIEETHTLPNASTFCGRCEAVCPVRIPLPKLMRHWREVQFEKGL
                     TPPTARYGLRAWGFVATRPALYRAVTRAAAAVLGLLGRGTGRFRHLPLAGGWTQSRDL
                     PAPSGTTFMAAWAKQAGRPSEPK"
     gene            4512..5210
                     /locus_tag="P5558_20930"
     CDS             4512..5210
                     /locus_tag="P5558_20930"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRY26846.1"
                     /translation="MSDAREAVMARIREALGRTPEEDARARERVAAWPREAPPGRIPA
                     RGQLEPEARIELFTSMATRVQTDVERVTGAGAIPAAVGRYLRRHNLPQKLVMAPDPVL
                     DEAGWASQPLLRIGRGTASDDDAVGLTLAVAGIAETGTVLLASSPDRPTLLAYLPETC
                     IIVLPTDRIVGAYEQALALYREDASGLPRSLNFITGPSRTGDIAQKLELGAHGPKRLL
                     VVLVDPESTAGQPA"
     gene            5207..5755
                     /locus_tag="P5558_20935"
     CDS             5207..5755
                     /locus_tag="P5558_20935"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRY26847.1"
                     /translation="MSHEPDRRRSRQPTAEELALWRHITRDTTPIGKMGPPAPPEPLR
                     PPSSPPPSPSPTPARPAVPVSQPPPRPQPASHGIDRRTLQRLGRGLVPIDARLDLHGM
                     TQEEAHRHLGFFLAHHQRRGARCILVITGIGERSTGGILRTMTPRWLAEPPNAQRVIA
                     HAPAQRHHGGAGALYVLLRRLR"
BASE COUNT          847 a         2281 c         1857 g          770 t
ORIGIN      
        1 cccgacacga cctcgaagac gggtgcctcg cccgcctgga gctgggcccg ggcctggccg
       61 accagtcgca ggccctgcca gcggatgcag atgctgccga tgatcgcggt cagcacggtg
      121 agggcgagcg tcggccacag gccgatcagc ccgccgacct ggatgaacac ccccacctcg
      181 gccaacggca cgaggatgaa gagcagcagc aagaaggttc cgagagacaa ggcccgccct
      241 gtttgttttt cgcgctgcca cattatataa cggcttgaac gccgagagcc accgtgcgcc
      301 agacagatcg gctcggtcgc cgccgttggt gcgaccttgc gtgaacgggg accatgtccg
      361 aaagcttcgc cttcatcgat atcatcttct tcgccatggt cgcagcgttc atcgcgttcc
      421 ggctcaggag cgtcctcggt cgccgcaccg gcaacgagcg gcgacgggcg aacccggtgc
      481 tgacgccgga tcgggccgcc gagcaccaga gcaacgacaa tgtcgtcccg ctgcccgacc
      541 gcaacgccag ccagcaggcc gaggagccga acttcgccga tatcggcgac ccggccatca
      601 agcagggcgt caccgaaatc cgcctggccg accacaagtt cgatctggac ggcttcctgc
      661 agggtgccaa ggcggcgttc ggcatcatca tcgaggcgtt cgccaacggt gacaaggccg
      721 cgctccggcc gctgctggcc gacgacgtct atgccagctt cgctgccgcc atcggcgcgc
      781 gcgaggcggc gggcgagcga ctctcgaccg agatcgtctc gatccgctcg gcggacatcg
      841 ccgccgccgg catgagcggc ccctatgcca ggctgacgat ccgcttcgtc accgagcaga
      901 tcaacgtcac ccatgacgcc accgacaacg tggtcgacgg cgatccgaca cggatcgacg
      961 aggtggtcga catctggacg ttcagtcgcg acacgcgcac gtccgacccg aactggcagc
     1021 ttatcgagac ccgcaccccc gactgagcga ccacccatga tcgatcgttc gccgagaccc
     1081 gggggaccct gggccggcac cctggtggcc ggccttttcc ttctcctgct caccgcctgc
     1141 ggcaagccgc ccgaagcgcc acccatggcg ctcgagccgg tcgccttcac cgcacttgcc
     1201 ggctgggccg acgatcgcca ggaccaggcc ctctcgagct tccttcgctc ctgcgcgccg
     1261 ctggcgcgcc gcgatccgga ggcaccgttc cacaaggccg agccggccgc cgggcgaaat
     1321 gccgactggc aggcgctctg tcgacaggct gcggcgaccc cggtggagcc ggccgccgcc
     1381 cgggcctttt tcgaaaccgg cttccagccc tatcgcgtgc tcggcgacgg cgagagcgaa
     1441 ggccttttca ccggctattt cgagcctttg ctgcacggcg ccgcccggcc caacggcacc
     1501 gcctcgatcc cgctccgggc cgccccgggc gacatcgtga cggtcgatct cggccagttc
     1561 gccgatgacc tcgacggccg caaggtgcgg ggtcgggtca ccggcgaccg cctcgaaccc
     1621 tatccgagcc ggggcgagat cgaacggggt gcgctcgatg gccgcgggct cgagctcctc
     1681 tgggtcgacg acccgatcga gaagttcttc ctgcagatcc agggctccgg cctcgtcgag
     1741 ctcgatgacg gccgcacgct tcgcgtcggc tatgccgacc agaacggccg cgcctaccgc
     1801 gccatcggcc gcgacctgat cgagctgggc gagctgacga aggaggaggt ctcgctgcag
     1861 accatcgagg cctggcttcg cgcccacccg gaccgggccc gggagatcat ggacaggaac
     1921 gcctcctacg ttttctttcg cgtgctgggc gaggcggggg cgctggaggg gcccgaaggt
     1981 gcccagggtg tgccgctcca ggccgaacgc tcgctcgccg tcgaccgcag cttcatcccg
     2041 ctcggcgccc cgctctggct cgagaccgag gcccccttcc ccgacggcga gcggccgttc
     2101 cgccggctgg tcgtcgccca ggataccggc ggggccatca agggtgccgt gcgcggcgat
     2161 gtcttctggg gtgcggggga cttggccgcc ttcgtcgcag ggcatatgaa gagccgaggc
     2221 agctactatc tcctgctgcc gaggaccgcc cggcccgtga gctgaggctg cggtcttccg
     2281 ccgaaggatc tccatgcgcc ccgccccctc tgccgccgcc ccggaccgcc cgcgcgtcgc
     2341 cctcttcgtc acctgcctcg tcgatctctt ccggccgagc atcggcttcg ccgcgatccg
     2401 cctgctcgag gctgccggct gcacggtcga ggtgccgaag gcccagacct gctgcggcca
     2461 gcccgccttc aacagcggcg accgggcgac cgccgaggcc attgcccggc aggtcctcga
     2521 caccttcgag ccctatgact acgtcgtcgc cccctccggc tcctgcgccg gcatgctgcg
     2581 ccgccatctg cccgagctct tcgacaccga cccggtggcc agggagcgtg cctcggtgct
     2641 cgccgagcgc tgccacgagc tcctgggctt cctcgtcgac atccgcggcc tcaaggaggt
     2701 ggcgagccac ttcgcgggca ccgtcaccta tcacgacagc tgctcgggcc tgcgcgagct
     2761 cggcgtcaag gcgcagccgc gccagcttct cctgggtgtg ccgggcgtcg atctggtcga
     2821 gatgcgcgag ccggaggtct gctgcggctt cggcggcacg ttctgcgtca agtacccgga
     2881 gatctcgacc cggatggtga gcgacaagac ggccgacatc gcggccaccg gcgccgacac
     2941 cctcgccgcc ggcgatctcg gctgcctcat gaacatggcc gggcggctgc agcgccaggg
     3001 cagcaaggtg cgcgcgtacc acgtcgccga gatcctggcc ggccccggcg ccggcacagc
     3061 cacgctgccc ggcatcggtg agcggcgccg ctgatgcagc cgacctcgct cgccttcgag
     3121 gccaaggcgc acgccgccct cgacaacccg gccctccagt cggccctcgg cagcatcaag
     3181 accggctggc aggccggccg cagccgggca gccgaggcgc tgcccgagtt cgaggcgctg
     3241 cgcgaccgcg gccgcgacat caagaaccac acgctccagc atctcgatct ctatctcgag
     3301 atgttcgaga cgaagctcgc cgccgccggt ggccagctcc acttcgcccg cgacgccgcc
     3361 gaggcgcgtg ccaccgtcct cgatctctgc cgcaccgccg aggcgcgcgc ggtcaccaag
     3421 agcaagagca tggtggccga ggagatcggc atcaacgagc atctcgaggc caacggcatc
     3481 gagcggatcg agaccgacct cggcgagtac atcatccagc tcaggaagga gccgccgagc
     3541 cacctcgtcg gcccggccgt gcacctgacc aaggaccagg tctccgacct cttcgccgag
     3601 caccatggcg gcccgcgcat cgaggacccg gcggcgctcg tcgccgaggc ccgccgcgtg
     3661 ctgcgcgaga aatatttcca ggccgatatc ggcatcaccg gcgccaactt cctcgtcgcc
     3721 gagaccggca cgatcgtcac cgtcaccaac gagggcaatg ccgagctgac ccagggcctc
     3781 ccgcgcaccc acatcgtcat caccagcctc gagaagctgg tgccgaccat agacgacgcg
     3841 ttcaccctgc tccgcctcct cgcccgctcg gccaccggcc aggaattctc cgcctacacc
     3901 acgctcgtca ccggcccgaa gcgcccggcc gacctcgacg gccccgaccg cctgcacgtc
     3961 gtcctcgtcg acaacggccg caccaagatg ctgggcaccg ccctgcagga gatgctccgc
     4021 tgcatccgct gcggcgcctg catgaaccac tgccccgtct atctcgccgc cggcggccac
     4081 gcctatggct gggtctacgt gggccccatg ggctcggtgc tgaccccgca attcgccggc
     4141 atcgaggaga ctcacacgct ccccaacgcg tcgaccttct gcggccgctg cgaggccgtc
     4201 tgcccggtcc gcatcccgct gccgaagctg atgcgccact ggcgcgaggt gcagttcgag
     4261 aaagggctga ccccgccgac ggcgcgctat gggctcaggg cctggggctt cgtggcgacc
     4321 cgccctgccc tgtaccgcgc cgtgacccgc gcggccgccg ccgtgctcgg cctcctcggc
     4381 cgcggcaccg gccgcttccg ccacctgccg ctggccggcg gctggaccca gagccgcgac
     4441 ctgccggccc cctcgggcac caccttcatg gcggcctggg cgaagcaggc cggccggccc
     4501 agcgagccca aatgagcgac gcccgcgaag ccgtcatggc ccgcatcagg gaggccctcg
     4561 gccgcacgcc cgaggaggac gcgcgcgccc gcgagcgggt cgccgcctgg ccgagggagg
     4621 ccccgcccgg ccgcatcccg gcgcgcggcc agctcgagcc ggaggcaagg atcgagctct
     4681 tcacgagcat ggcgacgcgc gtgcagaccg acgtcgagcg ggtcaccggg gcgggcgcga
     4741 tcccggcggc ggtcggccgc tatctccgcc gccacaacct gccgcagaag ctcgtcatgg
     4801 cgccggatcc ggtcctcgac gaggcgggct gggcgagcca gccgctcctg cgcatcggcc
     4861 gcggcaccgc cagcgacgac gacgccgtgg gcctgaccct ggccgtggcc ggcatcgccg
     4921 agaccggcac cgtgctcctc gcctcctcgc ccgaccggcc gacgctcttg gcctacctgc
     4981 ccgagacctg catcatcgtc ctgccgaccg accggatcgt cggcgcctac gagcaggccc
     5041 tggccctcta ccgcgaggac gcctcgggcc tgccccgctc gctcaacttc atcaccggcc
     5101 cgtcgcgcac cggcgacatc gcccagaagc tcgagctcgg cgcccacggc ccgaagcggc
     5161 tcctcgtcgt cctggtcgat ccggagagca cggccggcca gccggcgtga gccacgagcc
     5221 cgaccgccgg cgcagccgcc agcccacggc cgaggagctg gcgctctggc ggcacatcac
     5281 ccgcgacacc accccgatcg gcaagatggg cccgccggcg ccgcctgaac ccctgcggcc
     5341 tccgtcgagc cccccgccga gcccatcgcc gacacccgcc cgccccgccg tcccggtcag
     5401 ccagccgccg ccccggccgc agcccgccag ccacggcatc gaccggcgca ccctgcagcg
     5461 gctcggccgc ggcctcgtcc ccatcgacgc ccggctcgac ctgcacggca tgacccagga
     5521 ggaggcgcac cgccacctcg gcttcttcct cgcccaccac cagcgccggg gcgcccgctg
     5581 catcctggtc atcaccggca tcggcgagcg cagcaccggc ggcatcctgc gcaccatgac
     5641 cccccgctgg ctggccgagc cgcccaacgc ccagcgcgtc atcgcccacg ccccggccca
     5701 gcgccaccac ggcggcgcgg gggccctcta cgtcctgctc aggcggctgc gctag
//
LOCUS       DAOTFI010000278         5752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_16573_length_5752_cov_5.566790, whole genome shotgun sequence.
ACCESSION   DAOTFI010000278 DAOTFI010000000
VERSION     DAOTFI010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16573_length_5752_cov_5.566790"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="P5558_20940"
     CDS             complement(<1..1045)
                     /locus_tag="P5558_20940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRY26848.1"
                     /translation="MTGAFARESEEVLAALGVDPKIGLGDDEVTRRRKAHGPNRLRQR
                     RGKSVWAILFDQVRNLIFLLLAVAAVTSFVFSEAVQGAAIIVAMLINAAIGFVTEWRA
                     RRSMEALRRLERIEARVVRGGEERRIPAERLVPGDIVPLAAGDLVPADLRLVESSKLR
                     CDEAQLTGESVPVDKAIETLPGEPALAERSNMAFKGTFVASGAGLGVVVGIGRDTELG
                     RITDLVATAEASVTPLERQLDQLARRLLLVILIVAAAIAAVGVAAGRDLLLMIETGIA
                     LVVAAVPEGLPIVASVALARGMWRLARRNALIEKLSAVETLGSTTVICTDKTGTLTEN
                     RMTLRRLVLARGDV"
     gene            1163..2704
                     /locus_tag="P5558_20945"
     CDS             1163..2704
                     /locus_tag="P5558_20945"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY26849.1"
                     /translation="MKARQAPPVLAFAAVRKVFGGTVAVDDVTLDLARGEILALLGEN
                     GAGKSTMIKMLAGIHSPDGGAILFRGEPYAHRPPVAGEPQRVAFIHQDLGLIEWMTVA
                     ENMALGQGYERRFGLIDWRAAERRAAAALARVGLAGLEPSQRVQSLSRTEKSLLAIGR
                     ALAVEAEVLVLDEPTASLPADEVERLFDVLRDLRARGVAMIYVSHRLDEVFAIADRVA
                     VLRDGRLVGTRPVAETSADELIELIIGRRTAARHWRGVVAAGTTRLVVEGLATEEAGP
                     VDFTVAAGECVALVGLRGAGQVAIGRVLFGAAPRLAGRVRLDGREPDLETPRAAMASG
                     VSFVSGERVDESLLARLSMRENLFANPGARGRGLLELRRRRGEEAEAATLGERFDVRP
                     NEPERPIETFSGGNQQKIVMARWLAIAAPVLILEEPTAGVDVGAKIEIYELLAAARRA
                     GTAVIVVSTDFEEVERIADRALVFGRGRITAELSGDDLTQESVLHAASAVAGAWNDVA
                     TRGLD"
     gene            2705..3766
                     /locus_tag="P5558_20950"
     CDS             2705..3766
                     /locus_tag="P5558_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26850.1"
                     /translation="MQSLRSNALEPTRADLAGLGTAQRLARLLPVYGLPILTLALIVL
                     FSLILPDTFPTLLNLRSILGDKAIIALLSLAAMIPMMTGRIDLTVGYGIVLWHVLAIS
                     LQTQLGLSWPMAVLVVLLLGCLLGLLNGLLVEMAQIDSFIATLGTGTVLYALALWYTE
                     GRQIIGSLPPGFTGLYTTQILAIPIAAYYVLIVALLLWVIAEHLPIGRYLYAIGANPR
                     AAALNGIPTRRYVIGAFVASGCLCAVAGVVLAAKLRIGQASVGLEFLLPALVGAFLGS
                     TTIKPGRVNVWGTIVGVAVLAVGISGIQQLGGAFWVEPLFNGVTLVLAIGIAGYAQRK
                     RSTVRSLTRKPQPGGKNDA"
     gene            3759..4865
                     /locus_tag="P5558_20955"
     CDS             3759..4865
                     /locus_tag="P5558_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY26851.1"
                     /translation="MHRTLIAALAATLLGQTAWTTPAAADAVSDAKAYVEAASKPNPP
                     WDGPTEGPAAEAGKSIVYVSTDQRNGGARGVGVAVEEAAGVAGWTYRELDGQGTVSGR
                     SSALSQAMALQPDVIVLGGIDANEQAAAIEEAAAAGIVLVGWHSYAKAGPHDTLPIFT
                     NITTDPLEVARAAASLAVAESDGKAGAVVFTDSVYEIAVAKADAMAEIIKACETCTLL
                     EYVDTPLSDTATRMPPLTTALLQRHGDAWTHALSINDLTFDFMAPSLASAGIEGDGNP
                     RNISAGDGSEAAFQRIRDVQYQVGTVAEPLRLQGWQVVDEANRALAGEKDSGYVAPPH
                     LFTPDNIAFDGGPDNIYDPDNGYTDAYRKIWGAN"
     gene            4925..>5752
                     /locus_tag="P5558_20960"
     CDS             4925..>5752
                     /locus_tag="P5558_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26852.1"
                     /translation="MADHDLPKPDFARNMRLIGYSDQGGRSDGVQLMVWKGFAYTGHM
                     FSRGFSVIDVRDPRNPKPVSYHPAPANTWNIHLQTADDLLLVINAKDMFAAAEFADEH
                     AYYKGSLGDKVGTSAKASTDRDWTAGMAVYDISNAGEPKQIGFMPVDGGGVHRIWYTG
                     GRWAYASVLLDGYTDYIFMTIDMADPAQPKEAGRYWLPGMHTACGETPSWEPTRRFGL
                     HHAIIAGDTAYAAWRDAGLVMIDVKDRAKPELIVHKNWSPPFGGGTHNCLPLPDRELL
                     "
BASE COUNT          846 a         2200 c         1896 g          810 t
ORIGIN      
        1 cgacgtcccc tcgggccagc acgaggcggc gcagggtcat gcggttctcg gtgagcgtgc
       61 cggtcttgtc cgtgcagatc accgtggtgc tgccgagcgt ctccaccgcc gagagcttct
      121 cgatcagcgc gttgcggcga gccagccgcc acatcccgcg ggccagcgcg acgctggcga
      181 cgatcggcag gccctccggc accgccgcga cgaccagcgc tatgccggtc tcgatcatca
      241 gcagcaggtc gcgcccggca gccacgccga cggcggcgat ggcggccgcg acaatgagga
      301 tgacgagcag caggcggcgg gccagttgat cgagctggcg ctcgagcggg gtcaccgatg
      361 cctcggccgt ggcgacgaga tcggtgatcc ggccgagctc ggtgtccctg ccgatgccga
      421 ccaccacccc gagcccggcg ccgctggcga cgaacgtgcc cttgaaggcc atgttggatc
      481 gctcggccag cgccggctcg ccgggcagcg tctcgatggc cttgtcgacc ggcaccgact
      541 cgcccgtgag ctgcgcttcg tcgcatctca gcttgctcga ttcgaccagg cgcaggtcgg
      601 ccgggacgag atcgccggca gcgagcggca cgatgtcgcc cggcaccagc cgctcggccg
      661 ggattcgccg ctcctcgccg ccgcgaacga cccgcgcctc gatccgctcg agccggcgca
      721 acgcctccat cgagcgcctg gcccgccatt cggtgacgaa gccgatggcc gcgttgatca
      781 gcatggcgac gatgatcgcg gcaccctgca cggcctcgct gaagacgaag gacgtgacgg
      841 cggccacagc gagcagcagg aagatcaggt tgcggacctg gtcgaagagg atcgcccaga
      901 cgctcttgcc ccgccgctgg cgcagccgat tgggaccgtg cgccttgcgg cgtcgcgtca
      961 cctcgtcgtc gccaaggccg atcttcgggt cgacgccgag cgccgcgagg acctcctcgc
     1021 tttctcgggc aaaggctccg gtcattcgcg cggctttctc cgatcggcct ctggcgacat
     1081 tgccagcgag agtggcgagg cctatcaacg agggcaaggg aggctgaaat gtcgcaccgt
     1141 tcgatgccat gaaaagacgc ccgtgaaggc ccgccaggcg ccgcccgttc tggccttcgc
     1201 ggccgtgcgc aaggtgttcg gcggcacggt ggcggtggac gacgtgacgc tcgatctcgc
     1261 ccgcggcgag atcctggccc tgctcggcga gaacggcgcc ggcaagtcca ccatgatcaa
     1321 gatgctggcc ggcatccaca gccccgatgg cggcgccatc ctcttcaggg gcgagccata
     1381 tgcccaccgg ccgccggtcg ccggcgagcc gcagcgggtc gccttcatcc accaggacct
     1441 cggcctcatc gagtggatga ccgtggccga gaacatggcg ctcggccagg gctacgagcg
     1501 ccgcttcggc ctgatcgact ggcgcgcggc cgagcgccgc gccgccgccg ccctggcccg
     1561 ggtcggcctc gccgggctcg agccgagcca gcgcgtgcag agcctgtccc gcaccgagaa
     1621 gtcgctgctc gccatcggcc gggcgctggc ggtcgaggcc gaggtgctgg tgctcgacga
     1681 gccgaccgcg tcgctgccgg ccgacgaggt cgagcggctc ttcgacgtgc tccgcgatct
     1741 gcgcgcccgc ggcgtcgcca tgatctacgt ctcgcaccgg ctggacgagg tcttcgccat
     1801 cgccgaccgg gtggcggtgc tgcgcgacgg ccggctcgtc ggcaccaggc cggtcgccga
     1861 aacctcggcc gacgagctga tcgagctgat catcggccgc cgcaccgccg cccggcactg
     1921 gcgcggcgtg gtcgctgccg ggaccacccg cctcgtcgtc gagggcctcg ccaccgagga
     1981 ggcgggtccc gtcgacttca ccgtcgccgc cggtgagtgc gtcgccctcg tcgggctgcg
     2041 cggcgccggc caggtcgcca tcggccgtgt cctcttcggc gccgccccgc gcctcgccgg
     2101 gcgggtccgg ctcgacggcc gggagccgga tctcgaaacc ccgcgcgccg ccatggcgag
     2161 cggcgtctcc ttcgtctccg gcgagcgggt cgacgaaagc cttctggccc ggctctcgat
     2221 gcgcgagaac ctcttcgcca acccgggtgc ccgcgggcgc ggcctcctcg agctgcgccg
     2281 ccgccgcggg gaggaggcgg aggccgccac cctcggcgag cgcttcgacg tccgcccgaa
     2341 cgagcccgag cggccgatcg agaccttctc cggcggcaac cagcagaaga tcgtgatggc
     2401 ccgctggctg gcgatcgccg ccccggtgct gatcctcgag gagccgacgg ccggcgtcga
     2461 tgtcggcgcc aagatcgaga tctacgagct tctggcagcc gcccgccgcg ccggcaccgc
     2521 cgtcatcgtc gtctcgaccg atttcgagga ggtcgagcgc atcgccgacc gcgccctcgt
     2581 cttcggtcgc ggccggatca ccgccgagct cagcggcgac gacctcaccc aggagagcgt
     2641 cctgcacgcc gcctcggcgg tcgccggggc ctggaacgat gtagcgacga gggggctcga
     2701 ctagatgcag tcgctccgct ccaacgccct ggagccgacc cgggccgacc tcgccgggct
     2761 cggcaccgcg cagcgcctgg cccggctcct gccggtctac ggcctgccga tcctgacgct
     2821 cgctctcatc gtcctcttct cgctgatcct gcccgacacc ttcccgacgc tcttgaacct
     2881 gcgctcgatc ctcggcgaca aggcgatcat cgcgctcctg tcgctcgccg ccatgatccc
     2941 gatgatgacc gggcggatcg acctcaccgt cggctacggc atcgtcctct ggcacgtcct
     3001 cgccatcagc ctgcagaccc agctcggcct gagctggccc atggccgtgc tggtcgtcct
     3061 gctgctcggc tgcctgctcg ggctgctcaa cggcctgctc gtcgagatgg cgcagatcga
     3121 cagcttcatc gccacgctcg gcaccggcac cgtgctctat gcgctcgccc tctggtacac
     3181 cgagggccgg cagatcatcg gctcgctgcc gccgggcttc accggcctct acaccaccca
     3241 gatcctggcc atcccgatcg ccgcctacta cgtgctgatc gtggccctgc tcctctgggt
     3301 gatcgccgag cacctgccga tcggccgcta cctctacgcc atcggcgcca acccgcgcgc
     3361 cgcggcgctc aacggcatcc cgacccggcg ctacgtcatc ggcgccttcg tcgcctcggg
     3421 ctgcctctgc gccgtcgccg gcgtggtgct cgccgccaag ctgcgcatcg gccaggccag
     3481 cgtcgggctc gagttcctcc tgcccgccct ggtcggggcc tttctcggct cgaccaccat
     3541 caagcccggc cgggtcaatg tctggggcac catcgtcggc gtcgccgtgc tcgccgtcgg
     3601 catttccggc atccagcagc tcggcggcgc cttctgggtc gagccgctct tcaatggcgt
     3661 gacactggtg ctggcgatcg gcatcgccgg ctatgcccag cgcaagcgct cgaccgtccg
     3721 ctccctcacc aggaaaccac aaccaggagg caagaacgat gcataggacc ctgatcgcgg
     3781 cgctggccgc cacactgctc ggccagaccg catggaccac tccggccgcg gccgacgccg
     3841 tctcggacgc caaggcctat gtcgaggcgg cgagcaagcc caacccgccc tgggacgggc
     3901 cgaccgaggg cccggccgcc gaggccggca agagcatcgt ctacgtctcg accgaccagc
     3961 gcaacggcgg ggcgcgcggc gtcggcgtcg cggtcgagga ggcggcgggc gtcgccggct
     4021 ggacgtaccg ggagctcgac ggccagggca cggtctccgg ccgctcgagc gccttgagcc
     4081 aggccatggc gctgcagccc gacgtcatcg tgctcggcgg catcgacgcc aacgagcagg
     4141 ccgccgccat cgaggaggcc gccgccgccg gcatcgtgct ggtcggctgg cactcctatg
     4201 ccaaggccgg gccgcacgat accctgccga tcttcaccaa catcaccacc gacccgctcg
     4261 aggtggccag ggccgccgcc tccctcgccg tggccgagag cgacggcaag gccggggccg
     4321 tcgtcttcac cgacagcgtc tacgagatcg ccgtcgccaa ggccgacgcc atggccgaga
     4381 tcatcaaggc gtgcgagacc tgcaccctgc tcgaatatgt cgacacgccg ctcagcgaca
     4441 ccgccacccg catgccgccg ctgacgacgg ccctcctgca gcgccacggc gatgcctgga
     4501 cccatgcgct cagcatcaac gacctgacct tcgacttcat ggcgccgtcg ctcgcctcgg
     4561 ccggcatcga gggcgacggc aacccgcgca acatctccgc cggcgacggc agcgaggcgg
     4621 cgttccagcg catccgtgac gtccagtacc aggtcggcac cgtcgccgag ccgctccggc
     4681 tgcagggctg gcaggtggtc gacgaggcca accgcgcgct cgccggcgag aaggacagcg
     4741 gctacgtcgc cccgccgcat ctcttcacgc ccgacaacat cgccttcgat ggcgggccgg
     4801 acaacatcta cgacccggac aacggctaca ccgacgccta ccggaagatc tggggcgcca
     4861 actgaacaat gatgaccgcg gcgggcgccc aaccgggcgc ccgttcgccc gagggatgag
     4921 agacatggcc gaccacgacc tgccgaagcc cgacttcgcc cggaacatgc gcctgatcgg
     4981 ctacagcgac cagggcggca ggtccgacgg cgtgcagctc atggtctgga agggcttcgc
     5041 ctacaccggc cacatgttct ccaggggctt cagcgtcatc gacgtgcgcg atcccaggaa
     5101 cccgaagccc gtgagctacc accccgcccc ggccaacacc tggaacatcc acctgcagac
     5161 cgccgacgac ctgctgctcg tcatcaacgc caaggacatg ttcgccgccg ccgaattcgc
     5221 cgacgagcac gcctattaca agggctcgct cggcgacaag gtgggcacgt cggccaaggc
     5281 gagcaccgac cgcgactgga ccgccggcat ggcggtctac gacatctcga acgccggcga
     5341 gccgaaacag atcggcttca tgccggtgga cggcggcggc gtgcaccgca tctggtacac
     5401 cggcggccgc tgggcctacg cctcggtcct gctcgacggc tacaccgact acatcttcat
     5461 gacgatcgac atggccgatc ccgcgcagcc gaaggaggcc ggccgctact ggctgccggg
     5521 catgcacacg gcctgcggcg agaccccgtc atgggagccc acccgccgct tcggcctgca
     5581 ccacgccatt atcgccggcg acaccgccta cgccgcctgg cgcgacgccg ggctggtcat
     5641 gatcgacgtc aaggaccgcg cgaagcccga gctgatcgtc cacaagaact ggtcgccccc
     5701 cttcggcggc ggcacccaca actgcctgcc gctccccgac cgcgagctcc tc
//
LOCUS       DAOTFI010000279         5743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_16605_length_5743_cov_6.990506, whole genome shotgun sequence.
ACCESSION   DAOTFI010000279 DAOTFI010000000
VERSION     DAOTFI010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16605_length_5743_cov_6.990506"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="P5558_20965"
     CDS             <1..579
                     /locus_tag="P5558_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRY26853.1"
                     /translation="DLRTRVLDVPSQDVISRDNVSVKVNAVLYYRVLDADKAVIQVED
                     YDAATSQLAQTTLRSVLGQHELDEMLAERERLNNDIQTILDSQTDAWGIKVTNVELKH
                     VDIDESMVRAIAKQAEAERIRRAKVINAEGEYQAAERLVDAGRILNQVPEAMQLRYLS
                     TLHEIANDRSNTIVFPFPANLAEFLTGMKRDG"
     gene            complement(601..1290)
                     /locus_tag="P5558_20970"
     CDS             complement(601..1290)
                     /locus_tag="P5558_20970"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRY26854.1"
                     /translation="MAEPERLLVMIGVIARLLRGLGHVAVGASSPVPAAAALVARELG
                     RGRPTVSILGSETSNPFTDGGRELFDCAAQGRLDAFFLSGAQIDATGAVNLLGLGEPG
                     RMRRRFMGNFGAPYLASLVPNVILFRTDHGVRTLVEKVDFVTAPGVEGRWLVTDRAVF
                     RRQAGRLRLVSWHAGEMAETVRAATGFAFETAHDAGETPGLEAAAAAQLRGPVGAVLR
                     EVYPAFAAQLT"
     gene            complement(1283..2080)
                     /locus_tag="P5558_20975"
     CDS             complement(1283..2080)
                     /locus_tag="P5558_20975"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRY26855.1"
                     /translation="MTATPAELAALIPDGATLALAPDYSGCSLATIRELVRKGASGLR
                     LVGLPQLGLQADLLIAAGCVAGVETAAISLGELGLAPAFDRAWRAGSVTVTESTCPAI
                     HAGLTAAEKGVSHLPLAGVIGSDLVGLRPDWRLGPDPFTGEPGLLVPAIRPDFLLFHA
                     AVADRSGNVWIGVRRELMLAAHAARRTLATVEEVVAHDLLADDLWAAGTIPALYVERV
                     ATAPAGAAPVGLRNRYGPDRAWLAAYAEAARDPASCKTFIDAWLRDG"
     gene            complement(2077..2700)
                     /locus_tag="P5558_20980"
     CDS             complement(2077..2700)
                     /locus_tag="P5558_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRY26856.1"
                     /translation="MTESDVYARQGFGAVVGSGERPALLVVDFVNAFADPRMLGGGNI
                     EAAVAATVPVLRACRERSLPVVFTRIVYAEDGSDAGVWATKLPALRQLTPDSPANRVV
                     DALAPLPGELVIDKKQASAFFGTDLAPWLVQRRVDTLYVTGCTTSGCVRASVVDAVGW
                     NFRPIVIEDCVGDRAEGPHAANLFDMRQKYADLVTAEAAIRAIAGGA"
     gene            complement(2697..3524)
                     /locus_tag="P5558_20985"
     CDS             complement(2697..3524)
                     /locus_tag="P5558_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRY26857.1"
                     /translation="MGVRSGAATRGGIVAGRVAVGKSPVPAPGHAADFLLDRLLYRDG
                     LILVVDKPAGLPVHAGPKRGPNLEDYLEALRFGLPERPHLAHRLDRDTSGCLVLGRHR
                     KALRRLGRLFEAGRVEKVYWAVVEGTPDPAERRIELGLEKRSTAARGWWMQVAAEGGQ
                     ASVTDIRVLASGGGRSLVECRPRTGRTHQLRVHLAAIGHPILGDPVYGGPLQGSRQGG
                     PLLLHARSIALPLYPRRPAVAVTAPPPPAFVAAAGDLLPRGVAEPQTGPDTRIVETP"
     gene            3523..4734
                     /locus_tag="P5558_20990"
     CDS             3523..4734
                     /locus_tag="P5558_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRY26858.1"
                     /translation="MDLTPILLDWSALALRWLHLIAGIAWIGSSFYFMALDASLKKRA
                     GLPEGVSGETWQVHGGGFYRMWKYGVAPPEMPAELTWFKWEAYTTLLSGLALLVVVYY
                     LGAELYMIDPAKAELGPWTAAAIGLAVLVLGWVAYDMLCKSPLGNDQRTLALIGLVAF
                     PLVCLALDQVLTGRAAFLHVGALIGTIMVANVAMIIIPNQKKVVASLLAHAAPDPALG
                     AQAKQRSVHNNYLTLPVLFIMISNHYPQTFASPFNWLILALVLVIGASIRHYFNIMHA
                     GTKPPWWPWAVAALGFIVAIWIASFPATPPPLVDTAADERPATLAAVEEIVQGRCIMC
                     HGQETHWPGLAHAPKGVVLETPRDLRIHAAAIRDQVYQTHAMPPGNVSMIEDGERRLI
                     ARWVDAGAPPE"
     gene            4731..>5743
                     /gene="guaD"
                     /locus_tag="P5558_20995"
     CDS             4731..>5743
                     /gene="guaD"
                     /locus_tag="P5558_20995"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HRY26859.1"
                     /translation="MTRRALRGRLVWFRDDPAIAGAAALVDIADGLLVIEAERIAAVG
                     PATDLLEPDLEVTHYPNALILPGLIDAHIHYPQTQVIGSYGTQLLDWLQSYTFPAERR
                     FADPAHCARTAGFFLDELLRHGTTTAVAYASVHPGSVDALCAEALARTMRLITGKVMM
                     DEGPEGLRDTAQSGYDDSQALIARWHGQGRIGYAVTPRFAVTSSAAQLEAAGALLREH
                     PDCWLQTHLAENHAEIAEVARRFPEASSYTDVYDRFGLLGPRSILGHCLHLAEDEMAR
                     LAATGSVACFCPTSNLFIGSGLFDLARLTDRPDPVRIALATDVGGGTSYSMLQTAAEA
                     YKVL"
BASE COUNT          871 a         2216 c         1901 g          755 t
ORIGIN      
        1 gatctgcgca cccgcgtcct cgacgtgccg tcgcaggacg tgatcagccg cgacaatgtc
       61 tcggtcaagg tcaacgcggt gctctattac cgcgtgctcg acgccgacaa ggcggtgatc
      121 caggtcgagg actacgacgc cgcgaccagc cagctcgccc agaccacgct ccgctcggtc
      181 ctcggccagc acgagctcga cgagatgctg gccgagcgcg agcggctcaa caacgacatc
      241 cagacgatcc tcgacagcca gaccgatgcc tggggcatca aggtcaccaa tgtcgagctc
      301 aagcatgtcg acatcgacga gagcatggtg cgcgccatcg ccaagcaggc cgaggccgag
      361 cgcatccgcc gggccaaggt catcaacgcc gagggcgagt accaggcggc cgagcggctg
      421 gtcgatgccg gccgcatcct caaccaggtg ccggaggcca tgcagctccg ctacctctcc
      481 accctgcacg agatcgccaa cgaccgcagc aacaccatcg tcttcccgtt ccccgccaat
      541 ctcgccgagt tcctgaccgg catgaagcgc gacggatgat ccgacgcagc cgcggcggcg
      601 tcaggtgagc tgcgcggcga aggccggata gacctcgcgc agcacggcgc cgaccgggcc
      661 gcgaagctgt gccgccgctg ctgcttccag cccgggggtc tcgccggcgt cgtgcgccgt
      721 ctcgaaggcg aagcccgtgg cggcgcgcac cgtctcggcc atctcgccgg cgtgccagct
      781 caccagccga agccgccccg cctgccgccg gaagaccgcc cggtcggtca ccagccagcg
      841 cccctcgaca ccgggtgccg tcacgaaatc caccttctcg accagcgtcc ggaccccgtg
      901 atcggtgcga aagaggatga cgtttggcac cagcgacgcc aggtagggtg cgccgaaatt
      961 gcccatgaag cgtcgacgca tccggcccgg ctcgccgagg ccgagcaggt tcaccgcccc
     1021 cgtcgcgtcg atctgcgccc ccgagaggaa gaacgcatcg aggcgcccct gggcggcgca
     1081 gtcgaagagc tcgcgcccgc catcggtgaa ggggttcgac gtctccgagc ccaggatcga
     1141 gacggtcggc cggccccggc cgagctcgcg cgccaccagc gccgccgcgg ccggcaccgg
     1201 cgagctcgcc ccgacggcca catggccgag cccccggagc agccgggcga tgacgccgat
     1261 catcaccagc aggcgctccg gctcagccat cgcgcaacca ggcgtcgatg aaggtcttgc
     1321 aggacgccgg gtcccgcgcc gcctcggcat aggcggcgag ccaggcccgg tcggggccgt
     1381 accggttcct cagaccgacc ggcgccgccc ccgccggtgc ggtggcgacc cgctccacat
     1441 agagggccgg aatcgtgccc gcggcccaga gatcgtcggc gaggaggtcg tgcgccacca
     1501 cctcctcgac cgtggccagc gtgcgccgcg ccgcatgcgc cgccagcatc agctcgcgcc
     1561 gcacgccgat ccagacattg ccggaccggt cggcgaccgc cgcatgaaac agcaggaaat
     1621 cggggcggat ggcgggcacc agcaggcccg gctcgccggt gaaggggtcg gggccgaggc
     1681 gccaatcggg acgcaggccg acgaggtcgg agccgatcac cccggcgagc ggcagatgcg
     1741 agaccccctt ctcggcggcg gtcagcccgg catggatggc cgggcaggtg ctctccgtca
     1801 cggtgacgct gcccgcccgc cacgcccggt cgaaagccgg ggcgaggccg agctcgccca
     1861 gggagatggc ggccgtctcg acgccggcga cacagcccgc cgcgatgagc aggtccgcct
     1921 gcaggccgag ctgcggcagg ccgacgaggc gaagcccgct tgcccctttc ctgaccagct
     1981 cgcggatggt ggcgagcgag cagcccgaat agtccggcgc cagcgccagt gtggcgccat
     2041 cggggatcag cgccgcgagc tcggccggcg tcgcggtcac gcgccgcccg caatggcgcg
     2101 gatcgcggcc tccgcggtga ccaggtcggc atatttctgg cgcatgtcga agaggttggc
     2161 cgcatgcggc ccctccgccc ggtcgccgac gcagtcctcg atgacgatgg ggcggaagtt
     2221 ccaccccacc gcatcgacca ccgaggcgcg cacgcagccg ctggtcgtgc agcccgtgac
     2281 atagagcgtg tcgacgcgcc gctgcaccag ccagggggcg agatcggtgc cgaagaaggc
     2341 cgaggcctgc ttcttgtcga tgaccaattc acccggcagc ggggccaggg cgtcgaccac
     2401 ccggttggcc ggcgaatcgg gcgtgagctg gcggagcgcg ggcagcttgg tcgcccagac
     2461 tcccgcatcg gagccgtcct cggcatagac gatgcgcgtg aagacgacag gcagcgaacg
     2521 ctcgcggcag gcccggagca ccggcacggt ggcagcgaca gccgcctcga tgttgccgcc
     2581 gcccagcatc cttggatcgg cgaaggcatt gacgaagtcg accaccagaa gcgccggccg
     2641 ctcgccgctg ccgaccaccg cgccgaagcc ctggcgggca tagacgtcgc tctctgtcat
     2701 ggcgtctcca cgatgcgcgt gtcaggccca gtctgcggtt ccgcaacgcc ccttggcaag
     2761 aggtcgcccg ccgccgcgac gaaagccggc ggcggcgggg ccgtcacggc gacagccggg
     2821 cggcgcgggt agagcggcag ggcgatcgac cgggcgtgca gcagcagcgg gccgccctgc
     2881 cggctgccct ggagcgggcc gccatagacc gggtcgccca ggatgggatg gccgatggcc
     2941 gccagatgca cccggagctg gtgcgtgcgc ccggtgcgcg gccggcattc gacaagcgag
     3001 cggccgccgc cgctggccag cacccgaatg tcggtgacgg aagcctgccc gccctcggcc
     3061 gcgacctgca tccaccagcc ccgcgcggcg gtcgagcgct tctccaggcc gagctcgatc
     3121 cgtcgctccg ccgggtccgg cgtgccctcg accaccgccc agtagacctt ctcgacccgg
     3181 cccgcctcga agagccggcc gaggcgcctg agcgccttcc tgtggcggcc gaggacgagg
     3241 cagcccgagg tgtcgcggtc gagccgatgc gccagatgcg gccgctccgg caggccgaag
     3301 cgaagcgcct ccagataatc ctcgagattg gggccgcgct tcggcccggc atgcaccggc
     3361 agcccggccg gcttgtcgac gacgaggatc agcccgtccc ggtagagcag gcggtcgaga
     3421 aggaagtcgg cagcgtggcc gggcgccggc actgggctct tgccgaccgc taccctgccc
     3481 gccacgatgc cgccgcgtgt ggccgccccg gatcgaacgc ccatggattt gactcccatc
     3541 ctcctcgact ggtcggcgct ggcgctccgc tggctccacc tcatagccgg catcgcctgg
     3601 atcggcagct cgttctactt catggcgctc gacgccagcc tgaagaagcg cgccgggctg
     3661 ccggagggcg tctccggcga gacctggcag gtgcatggcg gcggcttcta ccgcatgtgg
     3721 aagtacggcg tcgccccgcc cgagatgccg gccgagctca cctggttcaa gtgggaggcc
     3781 tacaccaccc tcttgagcgg cctcgccctc ctcgtggtcg tctactatct cggcgccgag
     3841 ctctacatga tcgacccggc caaggccgag ctcggcccct ggacagcggc cgcgatcggc
     3901 ctcgccgtgc tggtcctcgg ctgggtcgcc tacgacatgc tgtgcaaatc accgctcggc
     3961 aatgaccagc gcacgctcgc cctcatcggc ctcgtcgcct tcccgctcgt ctgcctcgcc
     4021 ctcgaccagg tcctcaccgg ccgtgccgcc ttcctccatg tgggagcgct gatcggcacc
     4081 atcatggtgg ccaacgtcgc catgatcatc atccccaacc agaagaaggt ggtggcctcg
     4141 ctgctcgccc acgcggcgcc cgacccggcg ctcggggcgc aggccaagca gcgctcggtg
     4201 cacaacaact acctgaccct gcccgtcctc ttcatcatga tcagcaacca ctacccgcag
     4261 accttcgcga gcccgttcaa ctggctgatc ctggcgctgg tgctggtgat cggcgccagc
     4321 atccgccact atttcaacat catgcacgcg ggcacgaagc cgccctggtg gccctgggcg
     4381 gtcgccgccc tgggcttcat cgtcgccatc tggatcgcga gcttccccgc caccccgccg
     4441 ccgctggtcg acaccgccgc cgacgagcgg cccgccaccc tcgccgcggt cgaggagatc
     4501 gtccagggcc gctgcatcat gtgccacggg caggagacgc actggccggg cctggcccac
     4561 gcccccaagg gcgtcgtgct cgagaccccg cgcgacctgc gcatccacgc cgccgccatc
     4621 cgcgaccagg tctaccagac ccacgccatg ccgcccggca acgtctccat gatcgaggac
     4681 ggcgagcggc ggctgatcgc ccgctgggtc gacgccgggg cgccgcccga atgacccggc
     4741 gcgccctgcg cggccgtctc gtctggttca gggacgaccc ggcgatcgct ggtgcggcgg
     4801 ccctggtcga catcgcggac ggcctcctcg tcatcgaggc cgagcggatc gccgccgtag
     4861 gcccggccac cgacctcctc gagcccgacc tcgaggtcac ccattacccg aacgccctga
     4921 tcctgccggg cctcatcgac gcccatatcc actaccccca gacgcaggtc atcggctcct
     4981 acgggaccca gctcctcgac tggctgcaga gctacacctt cccggccgag cgccgcttcg
     5041 ccgacccggc gcattgcgcc cggacggctg gcttcttcct cgacgagctc ctgcgccacg
     5101 gcaccaccac cgccgtcgcc tatgccagcg tgcaccccgg ctcggtcgac gccctctgcg
     5161 ccgaggcgct ggcgcgcacc atgcgcctga tcaccggcaa ggtgatgatg gacgaggggc
     5221 ccgagggcct gcgcgacacc gcccagtccg gctacgacga cagccaggcg ctgatcgccc
     5281 gctggcacgg ccagggccgc atcggctacg ccgtcacgcc gcgcttcgcc gtcacctcga
     5341 gcgccgccca gctcgaggcg gctggagccc ttctgcgcga gcatcccgac tgctggctgc
     5401 agacccacct cgccgagaac cacgccgaga tcgccgaggt ggcgcggcgc ttccccgagg
     5461 cctcgagcta caccgacgtc tacgaccgct tcggcctgct cggcccccgc tcgatcctcg
     5521 gccactgcct gcacctcgcc gaggacgaga tggcgaggct ggccgcgacc ggctcggtcg
     5581 cctgcttctg cccgacctcg aacctcttca tcggctccgg cctcttcgac ctcgcccgcc
     5641 tcaccgaccg gccggacccg gtgcgcatcg ccctcgccac cgatgtcggc ggcggcacct
     5701 cctactcgat gctgcagacc gccgccgagg cctacaaggt cct
//
LOCUS       DAOTFI010000280         5741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_16614_length_5741_cov_6.955681, whole genome shotgun sequence.
ACCESSION   DAOTFI010000280 DAOTFI010000000
VERSION     DAOTFI010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16614_length_5741_cov_6.955681"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /gene="glpD"
                     /locus_tag="P5558_21000"
     CDS             complement(<1..808)
                     /gene="glpD"
                     /locus_tag="P5558_21000"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRY26860.1"
                     /translation="MAKRERFDLVVVGGGINGAGIVRDAAGRGLSALLVERDDLASHT
                     SSWSSKLIHGGLRYLEHYEFRLVREALIEREVLLRAAPHIIWPLTFVLPHSPEQRPAW
                     LVRLGLFLYDHLGGREILPASKAVDLAAPGPYGDPLQDWVKRGFTYSDCWVMDSRLVV
                     LNAIDAVERGAEVRTRTECTRAVREDAGWRVDLRCTRTGATRTVQARALVNAAGPWVA
                     RFLDENLGHRSTKRVRLVKGSHIVTRRLHDGAHPYILQNTDKRIVFVIPYE"
     gene            complement(915..1706)
                     /locus_tag="P5558_21005"
     CDS             complement(915..1706)
                     /locus_tag="P5558_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY26861.1"
                     /translation="MRQPLRLAARELEGPKGPPPLLVLHGLFGSQRNWQAHGKALARE
                     RRVFLVDLRNHGYSPWSDVMDYPAMAADVAALAERNGLERVSLLGHSMGGKVAMTLAL
                     DRPALVDRLIVADIAPVAYSHDFDAELDAMRGLDLASVDRRSAADAAMAAALPNGAVR
                     QFLLQNLDFDDGGKAHWRLNLAVLQSAIDTLTGWPGQDPTRRFDGPALAVRGEHSPYV
                     DEAGRAALRRLFPGVEMVTIPAVGHWLHVEAPRPFLGAVQRFLDS"
     gene            complement(1703..2941)
                     /locus_tag="P5558_21010"
     CDS             complement(1703..2941)
                     /locus_tag="P5558_21010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRY26862.1"
                     /translation="MLIAGMLRRLVNYGHLVVIDASGTVHEFKGEGGPRAVIRLTDRR
                     IEAALLWHPELALGEGYMDGRIVVEEGSIYDVIEIGSVNQARLGTGMVGRVATSIGYA
                     LRRFHQYNPAPRSRRNVAHHYDLSSTLYRMFLDADRQYSCAYFPKPGASLEEAQLAKK
                     RHLAAKLLLEPGQKVLDIGSGWGGLGIYLAQVDEVDVTGLTLSVEQHEYAMRRVQEGG
                     LDDRVRFLLQDYRATEGRFDRIVSVGMFEHVGINHYRQYFEMIRDRLADDGVAVIHSI
                     GRASGPWATADFIRKYIFPGGYIPALSEVLPVIEKTGLWVTDVEILRLHYADTLKAWR
                     ERFRARWDEAADLYDERFCRMWEFYLAGSEAFFRCMDGMVFQIQLARQRDAVPLTRDY
                     IAAEEARLAKAEPARYRLVA"
     gene            complement(3026..5260)
                     /gene="glgB"
                     /locus_tag="P5558_21015"
     CDS             complement(3026..5260)
                     /gene="glgB"
                     /locus_tag="P5558_21015"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRY26863.1"
                     /translation="MAETRKRTTAQHPLVESAELQAVVRGSHENPFGVFGLHEVGGGL
                     VIRTFQPGAVSVELVEAASGKVLTALERLDEAGFFAARVPRKKAPFAYRLLVDWGGGE
                     PPREIEDPYRFGTVLGEMDVYLLAEGRHLRLFEQLGAHPAEMDGVPGTRFAVWAPNAR
                     RASVVGPFNNWDGRRHPMRKRIECGCFELFIPHLQKGELYKYELVGPQGEMLPLKADP
                     VGFRAEHPPANASVVHGLVRHEWHDGDWLERRARAQSREAPISIYEVHLGSWMRVPEE
                     GNRYLSYTELAERLVAYVKEMGFTHIELMPVSEYPFDGSWGYQPVGLFAPTIRHGTPE
                     EFAGFVDACHAAGVGVLVDWVPGHFPTDAHGLANFDGTALYEHMDPRKGFHQDWNTAI
                     YNYGRSEVANFLHANALFWLEKFHIDGIRVDAVASMLYLDYSRKAGEWEPNIHGGNEN
                     LEAIAFLKRLNELVYGEHPGATSIAEESTAWPAVSRPVYLGGLGFGFKWNMGWMHDTL
                     HYMKEEPIHRRYHQHNLTFGLLYAFSENFVLPISHDEVVHGKGSLLGRMPGDRWQRFA
                     NLRAYLGFMWTHPGKKLVFMGAEFGQEREWNYEGSLDWHLLDDPLHKGAQLFVRDLNQ
                     LYRSTPALFELDCEPHGFEWIDAADTEQSVLSYLRKGTEPGSVVVVVCNFTPVVRQGY
                     RIGVPAPGQWAEVLNSDAERYGGGGVGTGPEVTAEAIPWHGRPHSIHLTLPPLATVAY
                     ALRP"
     gene            complement(5253..>5741)
                     /locus_tag="P5558_21020"
     CDS             complement(5253..>5741)
                     /locus_tag="P5558_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HRY26864.1"
                     /translation="TKTRLHGDYHLGQVLIAKGDVYILDFEGEPQRDLEERRAKTSPL
                     KDVAGMLRSLDYGAVAALDRFAERDPVHLEEVRSLAATWREAAMRRFLDSYREHVVGC
                     SSVPADRAAFDAVLELFLIEKAAYEIAYEAANRPKWLGIPVRGVLGLLDPEQRKGVFG
                     DG"
BASE COUNT          926 a         1903 c         2045 g          867 t
ORIGIN      
        1 tctcgtaggg gatgacgaag acgatgcgct tgtcggtgtt ctgcaggatg tagggatggg
       61 caccgtcgtg caggcgtctg gtgacgatgt gcgagccctt gaccagccgg acccgcttcg
      121 tgctgcggtg gccgaggttc tcgtcgagga agcgcgctac ccagggcccg gccgcgttga
      181 ccagggcacg ggcctgcacg gtccgggtgg cgcccgtccg ggtgcagcgg agatcgaccc
      241 gccagccggc gtcctcgcgg accgcacgcg tgcattcggt ccgggtgcgc acctccgccc
      301 cgcgctccac cgcgtcgatg gcgttcagca cgaccaaccg gctgtccatc acccagcagt
      361 cgctgtaggt gaagccgcgc ttcacccagt cctgcagggg gtcgccgtag ggcccggggg
      421 cggcgaggtc gaccgccttc gaggccggga ggatctcgcg gccgcccaga tgatcgtaga
      481 ggaagaggcc gaggcgaacg agccaggccg gccgctgctc ggggctgtgc ggcaggacga
      541 aggtcaaagg ccagatgatg tgcggggccg ccctgagcag cacctcgcgc tcgatcaggg
      601 cctcgcggac gaggcggaac tcgtaatgct cgagatagcg caggccgcca tggatcagct
      661 tggacgacca ggaggacgta tggctggcga gatcgtcgcg ctcgaccaag agcgcgctca
      721 gcccgcgccc ggccgcatcg cgaacgatcc cggcaccgtt gattccgccg ccgacgacga
      781 cgagatcgaa cctctcacgc ttcgccaaga gacctcccga cctgcgcgat aacgggctac
      841 cacgccgtcg gaacgttcac aagtgaaagc aaaccgcgcg catttcgcct ttcgcatcgt
      901 tcataaatga acactcagga gtcgaggaag cgctgcaccg cgcccaggaa gggtcgtggc
      961 gcctcgacgt gcagccagtg gccgacggcg ggaattgtga ccatctcgac ccccggaaag
     1021 agccgccgga gcgccgctct gccggcctcg tcgacatagg ggctgtgctc gccacgaacg
     1081 gccagggccg gcccatcgaa gcgccgggtc gggtcctggc ccggccagcc ggtgagcgtg
     1141 tcgatggcgc tttgcagcac ggcgaggttg agtcgccagt gggccttgcc gccatcgtcg
     1201 aagtcgagat tctgcaggag gaactggcgg acggcgccgt tcgggagcgc tgccgccatg
     1261 gcggcgtcgg cagccgatcg acggtcgacg gaggcgaggt cgaggccgcg catcgcgtcg
     1321 agctcggcat cgaaatcgtg gctgtaggcc accggggcga tgtcggcgac gatcagccga
     1381 tcgaccagcg ccggccggtc cagggccagc gtcattgcca ccttgccgcc catgctgtgg
     1441 ccgagcaggc tgacacgctc gagcccgttg cgctcggcca gggcggcgac gtcggcggcc
     1501 atggccgggt agtccatgac gtccgaccag ggcgaatagc catgattgcg caggtcgacg
     1561 aggaagacgc gccgctcccg cgccagcgcc ttgccgtgcg cctgccagtt gcgctgcgag
     1621 ccgaagagcc cgtgcaggac gagcaggggc ggcgggccct tcggcccctc cagctcgcgc
     1681 gctgccagcc tcaggggctg tctcaagcga ccagccggta gcgggcgggc tcggccttgg
     1741 cgaggcgcgc ctcctcggcg gcgatgtagt cgcgggtgag cggcacggcg tcccgctgcc
     1801 tcgcgagctg gatctggaag accatgccgt ccatgcagcg gaagaaggcc tcggagccgg
     1861 cgagatagaa ctcccacatc cggcagaagc gctcgtcgta gagatcggcg gcctcgtccc
     1921 agcgggcgcg gaaacgctcg cgccaggcct tcagcgtgtc ggcatagtgc aggcgcagga
     1981 tctcgacgtc ggtgacccag agcccggtct tctcgatgac cggcaggacc tcggacaagg
     2041 ccgggatgta gccgcccggg aagatgtact tgcggatgaa gtcggccgtg gcccaggggc
     2101 cggaggcgcg gccgatcgaa tggatcacgg cgacgccgtc atccgcgagc cggtcgcgga
     2161 tcatctcgaa atactggcga tagtggttga tcccgacatg ctcgaacatg ccgaccgaga
     2221 cgatccggtc gaacctgccc tcggtcgccc gatagtcctg caggaggaag cgcacgcggt
     2281 cgtcgagccc gccttcctgg acccggcgca tggcgtactc gtgctgctcg accgagagcg
     2341 tcagcccggt gacgtcgacc tcgtcgacct gcgccagata gatgccgagg ccgccccagc
     2401 cggagccgat atcgaggacc ttctggcccg gctcgagcag cagcttggcg gccagatgac
     2461 gcttcttggc gagctgcgcc tcctccaggc tggcgcccgg cttcgggaag taggcgcagg
     2521 agtactgccg gtcggcgtcg aggaacatcc ggtagagcgt gctcgagaga tcgtagtggt
     2581 gggcgacgtt gcgtctcgag cgcggtgccg ggttgtactg gtggaagcga cggagcgcgt
     2641 agccgatgct ggtggcgacg cggccgacca tgccggtgcc gagcctcgcc tggttgacgg
     2701 agccgatctc gatcacgtcg tagatcgagc cctcctcgac gacgatgcgg ccatccatgt
     2761 agccttcgcc gagcgccagc tcggggtgcc agagcagggc cgcctcgatc cggcggtcgg
     2821 tcaggcggat gaccgcccgg gggccgccct cgcccttgaa ctcgtgcacc gtgcccgagg
     2881 catcgatcac cacgagatga ccatagttga ccagccgacg gagcatcccg gcgatcagca
     2941 tgacggcacc cgctcgcttg cttcccccta cgatccggga gcatgactcc ttttcacagc
     3001 gatttcaacg gttagggtgc agaattcagg ggcgtagcgc ataggccacg gtggcgagcg
     3061 gcggcagggt cagatggatg gagtggggcc ggccgtgcca ggggatcgcc tcggccgtga
     3121 cctcgggacc ggtcccgacg ccgccgccgc catagcgctc ggcatccgag ttgaggacct
     3181 cggcccactg gccgggcgcc ggcacgccga tccggtagcc ctggcgcacg accggcgtga
     3241 agttgcagac cacgacgacg acgctgccgg gctcggtccc cttgcgcagg tagctgagca
     3301 cgctctgctc ggtgtccgcc gcatcgatcc actcgaagcc atggggctcg cagtccagct
     3361 cgaagagggc cggcgtcgag cgatagagct ggttgaggtc gcggacgaag agctgcgcac
     3421 ccttgtgcag cgggtcgtcg agcaggtgcc agtcgaggct gccctcgtag ttccactcgc
     3481 gctcctggcc gaactccgcg cccatgaaga cgagcttctt gcccggatgg gtccacatga
     3541 agccgagata ggcgcgcagg ttggcgaagc gctgccagcg gtcgcccggc atccggccga
     3601 gcagcgagcc cttgccgtgc accacctcgt cgtggctgat cggcaggacg aagttctcgg
     3661 agaaggcgta gagcaggccg aaggtcaggt tgtgctggtg gtagcgacga tggatcggct
     3721 cctccttcat gtagtggagg gtgtcgtgca tccagcccat gttccacttg aacccgaagc
     3781 cgagcccgcc gagatagacc ggccgcgaga cggcgggcca ggcggtggat tcctcggcga
     3841 tcgaggtggc gcccgggtgc tcgccgtaga ccagctcgtt cagccgcttg aggaaggcga
     3901 tggcctcgag gttctcgttg ccgccgtgga tgttgggctc ccactcgccg gccttgcgcg
     3961 agtagtcgag gtagagcatc gaggccacgg catcgacgcg gatcccgtcg atgtggaact
     4021 tctccagcca gaagagcgcg ttggcgtgga ggaagttggc gacctcgctg cggccgtaat
     4081 tgtagatcgc cgtgttccag tcctgatgga agcccttcct cgggtccatg tgctcgtaca
     4141 gggccgtgcc gtcgaaattg gcgaggccgt gggcgtcggt ggggaagtgg ccgggcaccc
     4201 agtcgaccag cacgccaacg ccggcggcgt ggcaggcatc gacgaagccg gcgaattcct
     4261 cgggcgtgcc gtggcggatg gtgggggcga agagcccgac cggctggtag ccccaggagc
     4321 cgtcgaacgg gtactcggag accggcatga gctcgatatg ggtgaagccc atctccttga
     4381 cataggcgac gaggcgctcg gcgagctcgg tgtaggagag gtagcggttg ccctcctcgg
     4441 gcacgcgcat ccaggagccg agatgcacct cgtagatgct gatcggcgcc tcgcggctct
     4501 gggcccgggc ccgccgctcg agccagtccc cgtcatgcca ctcgtgccgc acgaggccgt
     4561 gcaccacgga agcattggcc ggcgggtgct cggcacgaaa gccgacggga tcggccttca
     4621 agggcagcat ctcgccctgc gggccgacca gctcgtactt gtagagctcg cccttctgca
     4681 gatgcgggat gaagagctcg aagcagccgc actcgatccg cttgcgcatc gggtggcggc
     4741 ggccgtccca gttgttgaag ggcccgacca ccgaggcgcg gcgcgcattg ggcgcccaga
     4801 cggcgaaccg ggtgcccggc acgccgtcca tctcggccgg gtgggcgccg agctgctcga
     4861 agagcctgag atggcggcct tcggcgagca gatagacgtc catctcgccg agcacggtgc
     4921 cgaaccgata ggggtcctcg atctcccggg gcggctcgcc gccgccccag tcgaccagga
     4981 gccgataggc gaagggcgcc ttcttgcgcg gcaccctggc ggcgaagaag ccggcctcgt
     5041 cgagccgctc gagggcggtg agcaccttgc cgctcgccgc ctcgacgagc tcgaccgaga
     5101 cagcacccgg ctggaacgtg cggatcacga ggccgccacc cacctcgtgc aggccgaaga
     5161 cgccgaacgg gttctcgtgg ctgccgcgca ccaccgcctg gagctcggcc gactcgacca
     5221 gcgggtgctg ggcggtcgtt ctcttgcggg tctcagccat cgccaaaaac cccctttcgc
     5281 tgctcggggt cgaggaggcc cagcacgccg cgcaccggaa tgccgagcca cttcggccgg
     5341 ttggccgcct cgtaggcgat ctcgtaggcc gccttttcaa tcaggaagag ctcgagcacg
     5401 gcgtcgaagg ccgcccgatc cgccggcacg ctgctgcagc cgacgacatg ctcgcggtag
     5461 ctgtcgagga accgccgcat cgccgcctcg cgccaggtgg cggccaggga ccgcacctcc
     5521 tcgagatgca ccggatcgcg ctcggcgaac cggtcgaggg cggcgacggc gccatagtcg
     5581 agcgagcgca gcatgccggc gacgtccttg agcggcgacg tcttggcccg ccgctcctcg
     5641 agatcgcgct gcggctcgcc ctcgaagtcg aggatgtaga cgtcgccctt ggcgatcagc
     5701 acctggccca ggtgatagtc gccgtgcagc cgcgtcttgg t
//
LOCUS       DAOTFI010000281         5739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_16619_length_5739_cov_7.122449, whole genome shotgun sequence.
ACCESSION   DAOTFI010000281 DAOTFI010000000
VERSION     DAOTFI010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16619_length_5739_cov_7.122449"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="P5558_21025"
     CDS             <1..388
                     /locus_tag="P5558_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26865.1"
                     /translation="EEIERAKLREAMEQLKQKQHHEEDLQKAFAERDLHPEWQERLNR
                     AIANAVNQGKNELLVVRFPADWCTDKGRAINNFDPDWPKTLTGFAKRAIEVYEKELEP
                     LGYKAKAQVLNYPGGMLGEVGIFISW"
     gene            complement(393..1865)
                     /gene="pap"
                     /locus_tag="P5558_21030"
     CDS             complement(393..1865)
                     /gene="pap"
                     /locus_tag="P5558_21030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HRY26866.1"
                     /translation="MFESAAVEHVVDEATFKREVPLLRTALLEAQFRLREAQGFPVIV
                     LVTGQDMSGKGQVMHRLTEWLDPRYLRINAYVPPHGAELERPAMWRYWRDLPPAGEIG
                     LVFGSWYNDALRAALDDRDGSGLDDWINRINAFEGLLGDEGALILKFLLRISPEERKR
                     REKRLKKAAYPSRHVLEEWTDLGPLKKIRPVADEIVTRTSTALAPWLVLPADDPAYRD
                     LLFGRTIRTALEKRLEGSPPTREAPPALVPSLDGRTVLDALDLTLELDRKTYGERLPA
                     LQYRLAELTDSRAFDRCSLVCVFEGNDAAGKGGAIRRFTAALDPRRYRVWPIGAPTDD
                     EKRQAYLWRFWRRVPRLGRTAVFDRSWYGRVLVERVEGYASEPEWHRAYGEINEFEAE
                     LTRHGCVVVKFWLATSPEEQLRRFEARADTAWKQHKITPEDWRNRQKWDAYAQAVSDM
                     VDRTSTAIAPWTLVEAEDKRWARIKVLETIADRLERALSR"
     gene            2038..3645
                     /locus_tag="P5558_21035"
     CDS             2038..3645
                     /locus_tag="P5558_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26867.1"
                     /translation="MKGIAIMRRLARFSLAIALGMVVAGCIPLPPILPSGRQYEVEEV
                     LTLQASAGDRAAVHAAYGPPMASFLDGRLEAFYLTGDTGRLPFIAILPAVPVPVPLPG
                     IRRLGAEDHFIVVGYDDAGTVLGSVLWSTDDRHQVQGTHGSGTLAPLAQDRPTGRLWK
                     IVGWQTASIGQDADRPPSLPVVSPGGSLVALRTASDILVHASVDGGTVGRLYATTPLC
                     GRLQQPSGSEVEIARAELFSGEERLLVADMDGGLCSWEPATGLARRLFGDEGGGRAAG
                     GRWLAAIDATRVALADGQGAVAIQDRLTGALLVAGDPPMRLWGCTAPLGVTDGRHLVQ
                     SCLAGIDRRILLLRSTDDLSVLDIAEATAPSALPFVPSYFRPDRAGQLALDAAGGRVA
                     LLDGPFVRLLEVAADDSGSPRLQALSPILLSTALGATALEIPVGYRFYGANGGEPLQS
                     SDGRYLARPMPPVLAFSADGSRLVAGFVGCVVWDTATWREIWRCDAEDDDRRAELRRG
                     LALTPDGRHIMTNAGIYELPVSRVPPP"
     gene            3719..>5739
                     /locus_tag="P5558_21040"
     CDS             3719..>5739
                     /locus_tag="P5558_21040"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRY26868.1"
                     /translation="MTELLEVRLDDKYRKDRGRVFMTGVQALVRLPMEQHRRDAARGW
                     KTGGYITGYRGSPLGAYDQELQNVRPLLEAHHIHHQPGVNEDLAATACQGTQQVHMLG
                     PTSYDGVFAIWYAKGPGVDRSGDAIRHGHLFGTHPKGGVLLLLGDDHICESSTTAHQS
                     EFAMVDAMVPILNPSGVAEIFDYGLLGIAMSRFTGGWVSLKCVHDTVECTASAELDPE
                     RPAIVLPEETEVGPGPRHIDLVTDQSLPALAGELERRVHTTKLEAARAFARANHLDRI
                     THGAAGARLGIVTTGKSWLDVTAALETLGLDDARCRALGLAVYKVGMTFPLEPVGLER
                     AMAGVELAMVVEEKRGLVEEQMKGLLYAAAHRPRIIGKADERGAPLFPSHGQLDSNQI
                     AVAVARRLLERQDDAQLRERLAGLEAAAGASFTPAMARLPYFCAGCPHSSSTHVPEGS
                     RAMAGIGCHFMVNWMDRATSGFTQMGGEGGSWLGAAPFSDRPHLFQNMGDGTFYHSGS
                     MVVRAAVASRQGITLKILVNDAVAMTGGQKMEIGNLDVAAITRLLEAEGVREVVVVSD
                     EPGKYPIGTRMAPGVRLHHRDELDAVQRRLRGIEGVTAIVYDQTCAAEKRRRRKQGTM
                     VDPDERVVINEAVCEGCGDCGVQSNCVAILPVETELGRKRRIDQSACNK"
BASE COUNT          846 a         1999 c         2023 g          871 t
ORIGIN      
        1 cgaggagatc gagcgcgcga agctgcgcga ggcgatggag cagctcaagc agaagcagca
       61 ccacgaggag gacctgcaga aggccttcgc cgagcgcgac ctgcatcccg agtggcagga
      121 gcggctcaac cgggcgatcg ccaacgctgt caatcagggc aagaacgagc tgctggtggt
      181 ccgcttcccg gcggactggt gcaccgacaa gggccgcgcg atcaacaact tcgaccccga
      241 ctggccgaag acactgaccg gcttcgccaa gcgggcgatc gaggtctacg agaaggagct
      301 cgagccgctc ggctacaagg cgaaggcgca ggtcctcaac tatccgggcg gcatgctcgg
      361 cgaggtcggg atcttcatct cctggtaggg cctcaccgcg agagcgcacg ctcgagccgg
      421 tcggcaatcg tctccagcac cttgatgcgc gcccagcgct tgtcctcggc ctcgaccagg
      481 gtccagggtg cgatggcggt gctcgtccgg tcgaccatgt ccgagacggc ctgggcgtag
      541 gcgtcccatt tctgccggtt gcgccagtcc tcgggcgtga tcttgtgctg cttccaggcg
      601 gtgtcggcac gcgcctcgaa gcgccggagc tgctcctcgg gcgaggtggc cagccagaac
      661 ttgacgacga cgcagccgtg ccgggtgagc tccgcctcga actcgttgat ctcgccatag
      721 gcgcgatgcc actccggctc gctggcgtaa ccctcgaccc gctcgaccag caccctcccg
      781 taccaggagc ggtcgaagac ggcggtgcgg ccgagcctcg gcacccggcg ccagaagcgc
      841 cagagatagg cctgccgctt ctcgtcgtcg gtgggggcgc cgatcggcca gacccggtag
      901 cggcgaggat cgagtgccgc agtgaaacgg cgaatggcgc cgcccttgcc ggccgcgtca
      961 ttgccctcga agacgcagac cagcgagcag cggtcgaatg ccctggaatc ggtcagctcg
     1021 gcgagccggt actggagagc cgggagccgc tcgccgtacg tcttgcggtc gagctcgagg
     1081 gtgagatcga gcgcgtcgag cacggtcctg ccatcgaggc tcggcaccag cgccggaggc
     1141 gcctcgcgcg tcggcggcga accttcgagg cgcttctcca gggccgtgcg gatggtccgg
     1201 ccgaagagca ggtcgcgata ggccggatcg tccgccggca ggacgagcca tggggccagg
     1261 gccgtgctgg tccgggtgac gatctcatcg gccaccgggc ggatcttctt caagggcccg
     1321 aggtcggtcc actcctccag cacatggcgg ctcggatagg cggccttctt cagccgcttc
     1381 tcccgccgct tgcgctcctc gggcgagatg cgcagcagga acttgaggat cagcgcaccc
     1441 tcgtcgccga gcaggccctc gaaggcgttg atccggttga tccagtcatc gagcccgctg
     1501 ccgtcgcgat cgtcgagtgc tgcgcgcagg gcgtcgttgt accaggagcc gaagaccagc
     1561 ccgatctcgc cggccggcgg caggtcgcgc cagtagcgcc acatcgccgg ccgctccagc
     1621 tccgcgccat ggggcgggac ataggcgttg atgcgcagat agcggggatc gagccattcg
     1681 gtgagccggt gcatgacctg ccccttgccg gacatgtcct ggccggtgac caggacgatg
     1741 accggaaagc cctgcgcctc ccgcagcctg aactgggcct cgagcagcgc cgtgcggagc
     1801 aggggcacct cgcgcttgaa ggtcgcctcg tcgaccacat gctcgaccgc cgccgattcg
     1861 aacattccgg tccccctgtc agcacctgcg cccatttctg cgcgaactgg gcgcagcggg
     1921 tcaatcgatg gacaggccac tgctgcgccc atggggagtg gtggcggcgg gagcaaccga
     1981 tcaatgcaaa aagatgcatt cttgccgatg cgtggctagg atcagcgggc gtccgacatg
     2041 aagggcatcg cgatcatgcg ccggctggcc cgtttctctc ttgcgatcgc gctcggcatg
     2101 gtggtggcag gctgcatacc gttgccaccg atcctgccga gcggccggca gtacgaggtc
     2161 gaggaggtcc tgaccctgca ggcttcggct ggcgatcgtg cggcggtgca tgccgcctac
     2221 ggcccgccca tggcgagctt cctcgatggc cggctcgagg ccttctacct gaccggcgac
     2281 accggtcgat tgcccttcat cgcgatcctg ccggccgttc cggtgcccgt gccgctaccg
     2341 ggcattcgcc gactcggggc cgaggatcac ttcatcgtcg tcgggtatga cgatgccggg
     2401 accgtgctcg gctcggtgct ctggtcgacc gacgatcgac accaggtgca gggcacgcac
     2461 ggcagcggca cgctggcgcc gctcgcccag gatcggccca cggggcgact ttggaagatc
     2521 gtcggctggc agaccgcctc gatcgggcag gacgcggacc ggcccccctc gctgccggtc
     2581 gtctcgcccg gcggcagcct cgtggcgctg cgcacggcga gcgacattct cgttcacgca
     2641 agcgtcgacg gcgggaccgt aggccgtctc tatgcgacga cacccctctg cggccggctg
     2701 caacagccat cgggcagcga ggtcgagatc gcgcgggcgg agctcttctc gggcgaggag
     2761 cggctgctcg tcgccgatat ggacggcggc ctctgctcct gggagcccgc gaccggtctc
     2821 gcccgccggc tattcggcga tgaaggaggc ggtcgtgctg ccggcggacg ctggctggca
     2881 gccatcgacg cgacgcgcgt cgccctcgcc gacgggcaag gtgccgtcgc gatccaggat
     2941 cggctgactg gcgcgctcct ggtcgccggc gacccgccga tgcggctctg gggctgcacc
     3001 gcaccgctcg gcgtcaccga tggccgtcat ctggtgcagt cgtgcctcgc gggcatcgac
     3061 cggcgcatcc tgctgcttcg cagcaccgac gatctctcgg tcctcgacat cgcggaggcc
     3121 acggcgccga gtgccctgcc cttcgtgccg tcctacttcc ggccggatcg tgccggccag
     3181 ttggcgctcg atgcggcggg cggccgtgtc gccctcctcg acggtccatt cgtccggctg
     3241 ctcgaggtcg ctgccgacga ctccggcagc ccgcggctgc aggcgctttc gcccattctg
     3301 ctgtcgacgg cgctgggtgc gacggcactg gagatcccgg tcggctaccg gttctacggc
     3361 gcgaacggcg gcgagccgct gcagtcgagc gacggccggt atctggcgcg gcccatgcca
     3421 ccggtgctcg ccttttccgc ggacggcagc cgcctcgtcg ccggcttcgt cggctgtgtc
     3481 gtctgggaca ccgccacctg gcgcgagatc tggcgctgcg atgccgagga cgatgatcgg
     3541 cgggcggagc tgcgccgggg gctcgcgctg acgccggatg gccggcacat catgaccaat
     3601 gccggcatct acgagttgcc cgtgagccgc gttccgccgc cgtagcgatc cgcgcgccgg
     3661 gctttatgcc cggccccgcg gctgctatcc ctgaccggct gtatcgggga ggcgaagtgt
     3721 gacggagctg ctcgaggtcc ggctcgacga caagtacagg aaggaccggg gccgggtctt
     3781 catgaccggc gtgcaggcgc tggtgcgcct gccgatggag cagcaccgcc gcgatgccgc
     3841 ccggggctgg aagaccggcg gctacatcac cggctatcgg ggctcgccgc tcggcgccta
     3901 cgaccaggag ctgcagaacg tccggccgct gctcgaggcg caccacatcc accaccagcc
     3961 cggtgtcaac gaggacctcg ccgccactgc ctgccagggc acgcagcagg tgcatatgct
     4021 gggccccacc tcctatgacg gcgtcttcgc catctggtat gccaaggggc cgggcgtcga
     4081 ccgctccggc gatgcgatcc gccacggcca cctcttcggc acccacccca agggcggcgt
     4141 gctgctgctg ctcggcgacg accatatctg tgagagctcg accaccgcgc accagtccga
     4201 gttcgccatg gtcgacgcca tggtccccat cctcaacccc tcgggcgtcg ccgagatctt
     4261 cgactatggc ctcctcggca tcgccatgtc gcgcttcacc ggcggctggg tcagcctcaa
     4321 atgcgtccac gacacggtcg agtgcacggc ctcggccgag ctggacccgg agcgcccggc
     4381 gatcgtgctg cccgaggaga ccgaggtcgg cccggggccg cgccatatcg atctcgtcac
     4441 cgaccagtcc ctgccggcgc tggccggcga gctcgagcgg cgggtgcaca cgaccaagct
     4501 cgaggcggcc cgggccttcg cccgcgccaa ccacctcgac cggatcaccc acggcgcggc
     4561 cggggcccgg ctcggcatcg tcaccacggg caagtcctgg ctcgacgtga cggcagcgct
     4621 cgagacgctc gggctcgacg acgcgcggtg ccgggcgctg gggctcgccg tctacaaggt
     4681 cggcatgacc ttccccctgg agccggtcgg gctcgagcgc gccatggccg gggtcgagct
     4741 cgccatggtg gtcgaggaga agcgcggcct cgtcgaggag cagatgaagg ggctgctcta
     4801 tgccgccgcc catcggccgc ggatcatcgg caaggcggac gagaggggcg caccgctctt
     4861 cccctcgcat gggcagctcg acagcaacca gatcgccgtc gccgtcgccc gccgcctgct
     4921 cgagcggcag gacgatgcgc agcttcgcga gcgcctggcc ggtctcgagg ccgccgccgg
     4981 ggcgagcttc acgccggcca tggcgcggct gccctatttc tgcgctggct gcccgcacag
     5041 cagctcgacg cacgtacccg agggcagcag ggcgatggcc gggataggct gccacttcat
     5101 ggtcaactgg atggaccggg cgacctcggg cttcacccag atgggcgggg aagggggctc
     5161 gtggctcggg gcggcgccgt tcagcgaccg gccgcatctc ttccagaaca tgggcgacgg
     5221 cacgttctac cattcgggct cgatggtggt ccgggcggcg gtggcgtcgc ggcagggcat
     5281 cacgctgaag atcctcgtca acgacgccgt cgccatgact ggcgggcaga agatggagat
     5341 cggcaacctc gatgtggcgg cgatcacccg gctgctcgag gccgagggcg tgcgggaggt
     5401 ggtggtcgtc tcggacgagc cgggcaagta cccgatcggc acccggatgg cgcccggcgt
     5461 gcgcctccat caccgggacg agctcgacgc ggtgcagcga cggttgcgcg gcatcgaggg
     5521 tgtgaccgcc atcgtctacg accagacctg tgctgccgag aagcggcgcc gccgcaagca
     5581 gggcaccatg gtcgacccgg acgagcgggt ggtcatcaac gaggcggtct gcgagggctg
     5641 tggcgattgc ggcgtgcagt cgaactgcgt cgccatcctg ccggtcgaga ccgagctcgg
     5701 ccgcaagcgg cggatcgacc agtccgcctg caacaagga
//
LOCUS       DAOTFI010000282         5626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_17064_length_5626_cov_6.175731, whole genome shotgun sequence.
ACCESSION   DAOTFI010000282 DAOTFI010000000
VERSION     DAOTFI010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17064_length_5626_cov_6.175731"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="P5558_21045"
     CDS             <1..379
                     /locus_tag="P5558_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Bcr/CflA family drug resistance efflux
                     transporter"
                     /protein_id="HRY26869.1"
                     /translation="GMARIARFGLLALTTLGLALVAAGLASAGQPPLWLFMGLCFLCF
                     SAFGLLFGNLNAMAMRPVARVAGLGASLISSISSLVAFLWATAFGRFYDATVFPLATG
                     FVAAGIIGLLALHYAARSRAGEV"
     gene            complement(877..1626)
                     /locus_tag="P5558_21050"
     CDS             complement(877..1626)
                     /locus_tag="P5558_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26870.1"
                     /translation="MRRRLLSLLLLVLAGCHTAMPVQTSFLGSPNGVAQSPPHVTLVR
                     AAFQRKVTREGGGFVPDALVPDEDLFGKPGALSDPSCIAAGIQRTLPNARFVATGRFW
                     NTIVPGRERLPLAELLAPPLLERLTMEGVDYLVIASHRLRPTEYFAGEFILEGGIGWT
                     MEQKAAALLIDLASGALVDAAEARLQFGTYAFHTLVVLPLFAKTYPAENPCSKLGALV
                     ANRLEPEASGATPTFLVVAGEDDLYEAVSAP"
     gene            complement(1638..1964)
                     /locus_tag="P5558_21055"
     CDS             complement(1638..1964)
                     /locus_tag="P5558_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26871.1"
                     /translation="MAADGDRQPDLGVVGAGASRATIVAELGPPSTVENRPGGSVACT
                     HEYTIGDKPSYGRALGHAVANAITIGLRKIIGTPIEMNAGGAYRTAIVYDTSGQAQTI
                     NGTRLE"
     gene            complement(2186..3049)
                     /locus_tag="P5558_21060"
     CDS             complement(2186..3049)
                     /locus_tag="P5558_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26872.1"
                     /translation="MPRSVKNLPGIWGVSEQPLGDAGRPAAPLTGEAAVAAGAETGAL
                     ASITTGGMAAASTGPTPAALLAPLFLGVGLILAPVTAAVGAVGGAATRADPEEAAAAE
                     KSFNRALDERDNAQPLVASFIDEAERLAGRTLADCGAAALPAECELADGGSPSALLRL
                     SSLEHFEVEGRVQPKLRLLKTLDAEIRTPDRDGAVVWRSWRYRGVQRSYFELAADDAR
                     LLRGELAAAREALVAKAVQDIFLIGPVERHETELQPEGTVWTVVPAGISVEMVILTPR
                     DARPQDPAPPP"
     gene            3277..3519
                     /locus_tag="P5558_21065"
     CDS             3277..3519
                     /locus_tag="P5558_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26873.1"
                     /translation="MNNTLKMVLHELNDPPEGDRARISTRRHDVSCRVMRHNTLGLSR
                     ILRHRRLGCRGIRDISAEDVADSATKSSGCRGIRDV"
     gene            complement(3733..4017)
                     /locus_tag="P5558_21070"
     CDS             complement(3733..4017)
                     /locus_tag="P5558_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26874.1"
                     /translation="MARKRTSLEALLEVAEPTPPTQLRPKAAKAAPKRTIANKPIKQQ
                     TVYLPIPVYEQLRRLAFDEREKMHTLIMEGLDRVFQDRGLQSLAELEAKS"
     gene            complement(3992..4639)
                     /locus_tag="P5558_21075"
     CDS             complement(3992..4639)
                     /locus_tag="P5558_21075"
                     /inference="COORDINATES: protein motif:HMM:NF018691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRY26875.1"
                     /translation="MNVIAFVTQKGGSGKTTLCVNLAVAAEAAGGRCLILDMDPQGTA
                     EAWYHDREAASPKLVKLAADGLAEAVAAARRQGFTHVFIDTPGRDEPSVAAAIRIADF
                     CLVPCRPTPADMKATPATIATIRRLAKPAAFVLSQTPPRGFRIKEAEIGLAVLGPVAP
                     VTVVSRNVFQDAQGAGLGVGEFEPGGKAAQEIDELWGWLVKKMEKMQDGTQKNIA"
     gene            complement(4747..5052)
                     /locus_tag="P5558_21080"
     CDS             complement(4747..5052)
                     /locus_tag="P5558_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRY26876.1"
                     /translation="MIRHIVFFSAKDPADLPEIEAGLRRLETIAAADRLEVRRNLGQD
                     DLGNTVDLVVYGEFADAAALAAYKADPLYAEAIAIVRPLRELRVAADVETDAVTEGP"
     gene            complement(5073..>5626)
                     /locus_tag="P5558_21085"
     CDS             complement(5073..>5626)
                     /locus_tag="P5558_21085"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY26877.1"
                     /translation="EHKDTIYLTVIDRDGNACSFINSLYDSFGSGLVCPATGVIFHNR
                     GRAFSLAAGHLNEIAPRKRPMHTIIPGLAFKEGAIWTTFGVMGGDYQPVGHAHLLTNL
                     IDFGMDPQEAIDAPRAMAYPGPLQVERGVPAATVRRLAELGHAPVEAASPLGGGQAIM
                     VDRARGVLVGGSDPRKDGLALGY"
BASE COUNT          870 a         1973 c         1842 g          941 t
ORIGIN      
        1 cggcatggcc aggatcgccc gcttcggcct cttggccctc accaccctcg gcctcgccct
       61 cgtcgccgcc ggcctcgcca gcgccggcca gccgcccttg tggctcttca tgggcctctg
      121 cttcctctgc ttctccgcct tcggcctcct cttcggcaac ctcaacgcca tggccatgcg
      181 ccccgtcgcc cgcgtcgccg gcctcggcgc gtccctcatc tcctccatct ccagcctcgt
      241 ggccttcctc tgggccaccg ccttcggccg cttctacgac gccaccgtct tcccgcttgc
      301 gaccggcttc gtcgcggcgg ggatcatcgg gctactggcg ctgcactacg cggcccggag
      361 ccgggcaggg gaggtttgag gggaaatagg gtcaggctga acgacgcggg ccgcggcatg
      421 ttcgggtgtc ctccgatgcg agcgagaggg tcatcgtcgc tacccgtccg cgatttccaa
      481 acccacagtc gcgaacgtga gtccgcgcgt cgttgccttg tcgttgctca acgttcaccc
      541 gccggtgcgt ccggcggtag gcggtccaca tgctgccttc gggctgcata ttcgtttcgg
      601 cctccccgct ggtcagggtc gccgcaggct cggcgtcctc gcccgacggc gggcctcatc
      661 gtggttctgc cactcccgct cccagcgcct cgtcgccatc cgtggcctgg cggcatcctc
      721 tcctttgacc ggtcttccgc cattgagtct tccctcgaac gttcgagacc ggacttcatc
      781 acattcgggg cgcgatcaca tcgcgaagtc gcgcgtgagt ccgaagcgtc cccgtgcgca
      841 ggccgccgcg acgccgagag gccgcacgaa attctgtcag ggggcgctca ccgcctcgta
      901 gagatcgtcc tcgccggcga cgaccaggaa cgtcggggtc gcgccgctgg cttcgggctc
      961 gaggcggttt gcaacgagcg cacccagctt cgagcaggga ttctcggccg gataggtctt
     1021 tgcgaagaga ggcaggacga cgagcgtgtg aaatgcgtag gtgccgaatt ggagccgtgc
     1081 ttccgcggcg tcgaccagcg ctccgctcgc gagatcgatg aggagcgccg cagccttctg
     1141 ctccatggtc cagcctatgc cgccctcaag gatgaattcc ccggcgaagt actccgtcgg
     1201 acgcaggcga tgcgaggcaa tcactagata gtcgactcct tccatcgtca gccgctccaa
     1261 taagggcggg gccaatagct ccgcgagggg cagccgctca cggccgggca cgatggtgtt
     1321 ccagaaccgg ccggtcgcga cgaatcgagc gttcggcaac gtccgctgga taccggcggc
     1381 gatgcaactc gggtccgaca acgcgcccgg ctttccgaac agatcctcgt cggggaccag
     1441 cgcgtcgggc acgaacccgc cgccctcgcg cgtgaccttt cgctggaacg ccgcacgcac
     1501 gagcgtgacg tgcggtggcg actgcgcgac cccgttgggg ctccccaaga acgaggtctg
     1561 gacgggcatg gccgtatggc agcctgcgag cacgagcagc agcagcgata gtaggcggcg
     1621 acgcatcggt gcggatccta ctccagcctt gtcccgttga tcgtctgcgc ttggccggac
     1681 gtgtcgtaga cgatggccgt gcgataggcg ccgccggcgt tcatctcgat gggcgtgccg
     1741 atgatcttcc ggaggccgat ggtgatcgca ttggcgaccg catggccgag ggcccggccg
     1801 taggagggct tgtcgccgat cgtgtattcg tgggtgcacg cgaccgagcc gcccggccgg
     1861 ttctcgacgg tcgagggcgg cccgagctcg gccacgatcg tggcgcggct ggcgccggcg
     1921 ccgacaacgc ccaggtccgg ctgacggtcg ccgtcggctg ccatgtagac cgagcatccg
     1981 gcgagcgtga gacgcgcggc cccgcacgct acgcagcccg ccaccgccac cgccggtagg
     2041 tctgccgacc cgcactgcag ctcacgggcc agtcgctcca gctcggcctc ctttgcgctc
     2101 ccgcacccgg gttggtgcca tggcgacacc gccctaacgc ccgaatccag cgccatcgat
     2161 gccggcatcg ccggtcttgc cgccctcaag gcggcggagc cgggtcttgg gggcgcgcgt
     2221 cacgcggagt gaggatgacc atttctactg aaatacccgc cggcaccacc gtccagacgg
     2281 tgccttcggg ttgcagctct gtctcatggc gttccaccgg tccgatcagg aagatgtcct
     2341 gaactgcctt ggcgaccagt gcctcacgcg ccgcggcgag ctcgccccgg agcagccgag
     2401 catcgtcagc ggcgagctcg aagtagcttc gctggacgcc tcgatagcgc cagcttctcc
     2461 agaccacggc accatcccgg tcgggagtcc gaatctccgc atcgagcgtt ttcagcaggc
     2521 gaagcttggg ctgaacgcga ccttccacct cgaagtgctc caatgaggaa aggcgcagca
     2581 gagcgctcgg gctcccccca tcggcgagct cgcactcggc gggcagggcg gccgctccgc
     2641 agtccgccag ggtccggccg gccaggcgct cggcttcgtc gatgaaggac gcaaccagcg
     2701 gctgcgcatt gtcgcgctcg tcgagcgcgc ggttgaagct cttctcggcg gcagcggcct
     2761 cctccgggtc ggctctcgtg gcggcgccac caactgcccc cacggcagct gtcaccggtg
     2821 ccagaatgag cccgacgccg agaaagagcg gcgcaaggag cgcggccggt gtcggacccg
     2881 tagacgctgc agccatcccc ccggtcgtga tgctcgcaag agccccggtc tcggcgccgg
     2941 ccgctactgc cgcttcgccg gtgagtggtg cggccggtct tcccgcatcg ccaagtggct
     3001 gctccgacac gccccagata cccggcagat ttttgactga gcggggcacc gacggtatct
     3061 cgatcgctcg cgtcccgcag gaagcgactg acagggccaa gaggccgccc aggagaacac
     3121 gccctcgccc gaccatgctc tggaccccca aatacccatg ctgctatcgc atgatcgacc
     3181 ggtctcgcta ttgatctggc tcaaggccgg ccgacggtcg ccaagcctgt tgcatgtgta
     3241 taggtggttc cgacatcttt gcggcaggct ggagccatga acaacacgct gaagatggtc
     3301 ttgcacgagc tgaacgatcc gccagaaggt gatcgggcgc ggatctcgac gcggcggcac
     3361 gacgtttcct gtcgcgtcat gcgccacaat acccttgggt tgtcgcggat tctgcgacat
     3421 cgccgcttgg gatgtcgcgg tatacgcgac atttccgccg aagatgtcgc ggattccgca
     3481 acaaaatcgt cgggatgtcg cgggatccgc gacgtttgac agcgaacgaa cccacggaat
     3541 cgacgatcga acccgggaaa tcgacgagcg aacccaagaa tttcccgaaa cgaacccaag
     3601 gagagccggc atttccggcc cctcccgacg aacgaacccg aaaaacccga aaggaacgag
     3661 cgaacccgaa ggccctcgac aggcggcaag aattaggcta taaatcctac gacgtggtgg
     3721 cctgcacagc gctcaggact tcgcctcgag ctcggcgagg gactgcaggc cgcggtcctg
     3781 gaagacccgg tcgaggcctt ccatgatcag cgtgtgcatc ttctctcgct cgtcgaaggc
     3841 gaggcggcgc aactgctcgt agacgggaat gggcagatag acggtctgct gcttgatcgg
     3901 cttgttggcg atggtccgct tgggtgccgc cttcgccgcc ttcgggcgaa gctgggtcgg
     3961 cggggtcggc tcggcgacct cgagcagggc ctcaagcgat gttcttttgc gtgccatcct
     4021 gcatcttctc catcttcttc accagccagc cccagagctc gtcgatctcc tgggccgcct
     4081 tgccgcccgg ctcgaactcg ccgaccccga gcccggcccc ctgcgcgtcc tggaagacgt
     4141 tgcgcgagac caccgtcacc ggcgccacgg gcccgagcac cgcgaggccg atctccgcct
     4201 ccttgatgcg aaagccccgt ggcggcgtct ggctcaggac gaaggccgcg ggcttggcga
     4261 ggcggcggat cgtggcgatg gtcgccggcg tcgccttcat gtcggccggg gtcggccggc
     4321 aggggacgag gcagaaatcg gcgatgcgga tggccgccgc caccgacggc tcgtcgcgcc
     4381 ccggcgtgtc gatgaagaca tgggtgaagc cctgccggcg ggcggcagcc acggcctcgg
     4441 ccagcccgtc ggcggccagc ttgaccagct tcgggctcgc cgcctcgcgg tcgtggtacc
     4501 acgcctcggc cgtgccctgc gggtccatgt cgaggatcag gcagcgcccg cccgccgcct
     4561 ccgccgccac cgcgaggttg acgcaaagcg tggtcttgcc gctgccgccc ttctgggtga
     4621 cgaaggcgat gacgttcatg ggcaggaagc ctgtccgcgt atcgtggtct gctgttgtca
     4681 gacctaccgc gaagcgggtc ggcttaacaa gtcatgtgag ccgtgaaggg caagtgcctc
     4741 tccggactat ggcccttcgg tcacggcgtc ggtctcgaca tccgccgcca cgcgcagctc
     4801 gcgcagcggc cggacgatgg cgatcgcctc ggcatagagc ggatcggcct tgtaggccgc
     4861 gagcgctgcc gcatcggcga actcgccata gaccacgaga tcgaccgtgt tgccgagatc
     4921 gtcctgcccg agattgcgcc gcacctccag ccggtcggcc gccgcgatcg tctcgagccg
     4981 cctcagcccg gcctcgattt ccggcagatc ggccgggtcc ttcgccgaga agaagacgat
     5041 gtggcggatc atcggcgcgt tcctctgggt cctcagtagc cgagcgccag cccgtccttg
     5101 cgcgggtccg agccgccgac cagcacaccc cgggcccgat ccaccatgat cgcctgcccg
     5161 ccgccgagcg gcgaggccgc ctcgaccggc gcatggccca gctccgcgag ccgccgcacc
     5221 gttgcggccg gcacaccgcg ctcgacctgc agcggccccg gataggccat ggcccgtggc
     5281 gcatcgatcg cttcctgcgg gtccatgccg aagtcgatga ggttggtcag caggtgcgca
     5341 tggccgactg gctggtagtc gccgcccatc accccgaacg tcgtccagat ggcaccttcc
     5401 ttgaaggcga ggcccggaat gatggtgtgc atcggccgct tcctgggcgc gatctcgttc
     5461 agatgacccg ccgccaagct gaaggcgcga ccccggttgt ggaagataac gccggtcgcc
     5521 gggcagacca ggcccgagcc gaagctgtcg tagagcgagt tgatgaagga gcaggcattg
     5581 ccgtcccggt cgatcaccgt gaggtagatc gtgtccttgt gctccg
//
LOCUS       DAOTFI010000283         5581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_17245_length_5581_cov_7.301484, whole genome shotgun sequence.
ACCESSION   DAOTFI010000283 DAOTFI010000000
VERSION     DAOTFI010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17245_length_5581_cov_7.301484"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1143)
                     /locus_tag="P5558_21090"
     CDS             complement(115..1143)
                     /locus_tag="P5558_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26878.1"
                     /translation="MEGGGANRAAAAAFGLFVLVALGCLALIAADAAVFRRVNAWYLA
                     LPQTNYVELVRVDEFRTSLKVGSFRAHMMIMMAQHAFFGALVAWLVCCWWPARGRRPD
                     ATPARGGTGLALGLCALAGSAILAYALACFISVPGSAGVCALYAKEGVIDLGHALGFL
                     LAAGLLWLAAWRFARGKRHLPGNGYIALGAALAGAAAFVVGMEEISWGQTYLAWQSPE
                     FFMTYNDQQETNAHNFLNGYLTPIYYAMGATILLGTIAAFVLDDLFGERLPWLRALCP
                     PRALLWVAIWFPLGAEIFIFSSTETFETLLTIYALGYGVAILARSAAACRDAVTEDGR
                     PPAIGQTA"
     gene            complement(1298..2221)
                     /locus_tag="P5558_21095"
     CDS             complement(1298..2221)
                     /locus_tag="P5558_21095"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRY26879.1"
                     /translation="MKVGVIGAGMVGGAAANALVLAGVASEIVLVDRDRKRAIAEAED
                     ILHATPFAHSARVQAGDYADLAGATVVIVAAGVSQRPGETRLELLARNTDVFAAIIPP
                     VLAAAPDAVLLIATNPVDVMTMVAARLSRLPAARVVGSGTILDTARFRALLGQHLGVS
                     PQSVHAHVLGEHGDSEVLCWSSADVGSIAVSEYGRQVGRPLDDAARQRIDEAVRRAAY
                     RIIEGKGATWFGIAGGLVRICRAIRGDERAVLTVSMLGDEVEGVGPVALSLPRVVGGS
                     GIVGTLWPSLTPAESAALAQSARVIVAAAAG"
     gene            2690..3652
                     /locus_tag="P5558_21100"
     CDS             2690..3652
                     /locus_tag="P5558_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HRY26880.1"
                     /translation="MSVGLLGLIDDVVGIAKIAAASIDDAAGQAARAGAKAAGLVVDD
                     TAVTPRFLVGFAAERELPIIAKITVGSLRNKLVFLLPAALLLSLVAPWAITPLLMVGG
                     LYLSFEGAEKVMEALGWHPGGHGSEAAEGDDAVPAPLLSAGEEARRVKGAIRTDFILS
                     AEIMAITLATVAASPFVTRAIVLAIVGVVITLAVYGVVALIVKADDAGLLLAARDGPV
                     SGPLGRGLVRVMPHFLQLLSFVGTAAMLWVGGGIILHGLEELGWRTPAGIVHHAGEVA
                     AQALGALGPIAGWLVAAIASGALALGLGAVTAVVMQRLVPAAVH"
     gene            complement(3669..4931)
                     /locus_tag="P5558_21105"
     CDS             complement(3669..4931)
                     /locus_tag="P5558_21105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY26881.1"
                     /translation="MTTSVGKVLAAVRSLLAGGGAFIVGNSLLGIVLPLRMAAAEYPV
                     MLIGLVMAAYYAGLALGGNYGKRIILRVGHIRAFAAFAAVLATTALAYPMLFTAVAWI
                     ALRFINGFCIAGLQATIESWLNDRSSNATRGRVLGLYMMTFYLAVASGQVLVNVADVD
                     GPELFMLAAALIALALVPVALTSLGAPALAGHRRLSVRTLYANSPVGVVGAGVAGMLV
                     GSFYGLGVVFARGIGLDVAQSALFMTMVVVGGFGFQWPIGMLADRFDRRIVLAAVLTM
                     VGVAWAFVAFASVSALSLPTLLGLAVLFGGAISSVYPICVAQAFDRLDRAHYVSASGR
                     LLMIYALGAMAGPLLASAVMAVRGPYSFFAFESVVAVGFAVFVGYGVWRRPPIPADQQ
                     EPFVAVSSVTPVAADLDPRCDGPAARPV"
     gene            complement(4946..>5581)
                     /locus_tag="P5558_21110"
     CDS             complement(4946..>5581)
                     /locus_tag="P5558_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26882.1"
                     /translation="IVVDEAHHLLPRTWGHAAAVLPQRLQEMLLVTVHPDHVAPEILR
                     PIDVAVAIGRSPDQTLTELARATDRAFIWPSGLDHEPGRVVAWFLDGGQPPFSMAAQP
                     SRAERLRHHRKYAEGNLRWHSFYFRGPDGRHNLRAQNLVVFCQIAQGIDEATWMFHLR
                     RGDYARWFRHAVKDEVLAAETERIERRGDIEPWQTREIICKLVDARYTLPE"
BASE COUNT          887 a         1939 c         1985 g          770 t
ORIGIN      
        1 gctgcggcta ttctatcgaa aaggcgcagg acattcattc acaggctctg agcgccgagg
       61 accggcggga cgtcgggcgg tcccgggtct ccggggccgt tctggcgttc gcggctaggc
      121 tgtctggccg atcgccggcg gccgtccgtc ttcggtcacc gcgtcgcgac acgccgccgc
      181 ggagcgggcc aggatggcca cgccatagcc tagcgcatag atcgtcagca acgtctcgaa
      241 ggtctcggtc gacgagaaga tgaagatctc ggcgcccagc gggaaccaga tcgccaccca
      301 gaggagcgcg cggggcgggc agagcgctct gagccagggc aaacgctcgc cgaagagatc
      361 gtcgaggacg aaggcggcga tcgtgccgag caggatggtg gcgcccatcg cgtagtagat
      421 cggcgtcagg tagccgttca ggaagttgtg cgcgttggtt tcctgctggt cgttgtaggt
      481 catgaagaac tcgggcgact gccaggcgag gtaggtctgg ccccagctga tctcctccat
      541 gccgacgacg aaggcggcag ccccggccaa agcggcgccg agcgcgatgt agccgttgcc
      601 gggcaaatgc cgcttgccgc gcgcgaagcg ccaggcggcg agccagagga ggccggcggc
      661 caggaggaag ccgagggcat ggccgaggtc gatcacgcct tccttggcgt agagcgcgca
      721 gacgccggcg ctgccgggca cgctgatgaa gcaggcgagc gcgtaggcga ggatggccga
      781 tccggcgagg gcgcagaggc cgagcgcaag gccggtgccg ccccgtgccg gggtggcgtc
      841 cggccgtcgg ccacgggccg gccaccagca gcagaccagc caggcgacga gcgcaccgaa
      901 gaacgcgtgc tgcgccatca tgatcatcat gtgcgctctg aagctcccga ccttcaggct
      961 ggtgcggaac tcgtcgaccc tgaccagctc gacgtagttg gtctgcggca gggccagata
     1021 ccaggcattg acccggcgga agacggccgc atccgccgcg atcagcgcca ggcagccgag
     1081 ggcgacgagg acgaacagcc cgaaggccgc agccgctgct cgatttgcgc caccaccttc
     1141 catcgccctg attcccgcgc tagtgccgga cggtcaatgc cccggcggct ggcgaagcat
     1201 cgcccgcccg gcggaaaacg caggttaacg cacggcgatc gtttgatgcc atgcgtcctc
     1261 tgatacgctt ggatgtcgag gatcgccggt tcgggggtca gcctgcggcg gcggcgacga
     1321 tcacccgggc actctgcgcc agggctgcgc tctcggccgg ggtcaggctc ggccacagcg
     1381 tgccgacgat tccgctgccg ccgaccaccc tcggcagtga cagggccacc gggcccaccc
     1441 cttccacctc gtcgccgagc atcgagaccg tgagcacggc ccgctcgtcg ccgcgaatcg
     1501 cccggcagat gcggaccagg ccgccggcaa tgccgaacca ggtggcaccc ttgccctcga
     1561 tgatccgata cgcggcacgg cgcacggcct cgtcgatgcg ctgcctggcg gcgtcgtcga
     1621 ggggcctgcc gacctgtcgc ccgtactcgc tgacggcgat ggagccgaca tcggcgctcg
     1681 accagcagag cacctcgctg tcgccgtgct cgcccagcac atgggcatgg accgactgcg
     1741 gcgagacgcc gagatgctgg ccgagaagtg cgcgaaagcg ggccgtgtcg aggatcgtgc
     1801 cggagccgac gacccgagcc gcgggcaggc gcgagagccg ggcagcgacc atcgtcatca
     1861 cgtccaccgg gttggtggcg atcagcagga ccgcgtcggg tgctgccgcc aggaccggcg
     1921 ggatgatggc ggcgaagacg tcggtgttgc gcgccaggag ctcgagccgc gtctcacccg
     1981 gccgctgcga cacgccggcg gcgacgatga cgacggtggc acccgccagg tcggcatagt
     2041 cgcccgcctg gaccctggcg ctgtgggcga agggcgtcgc gtgcaggatg tcctcggcct
     2101 cggcgatcgc gcgcttgcgg tcacggtcca cgagcacgat ctcgctggcg acgccggcca
     2161 gcaccagggc gttcgccgcg gcaccgccga ccatcccggc tccgatgacc ccgaccttca
     2221 tggtgctcgc tccggttgtc cgccgtcggt gcggcatgct cgtcggtcga tccggcgcgg
     2281 tcaacccgga ctcggccgcc ggccgggccg ccttggcccg gggactcgcg gtgcgcctgg
     2341 ctccgatact tccagaaccg gggaggaggc aggcagcaat ccaccggccg gtggactcgc
     2401 ttcctgccta tcccacctcg ccgcgttcct cgcccaggcg gacatgattg tcaccggccc
     2461 gctggagagc accggcttca acctcggcaa tggcatggcg tcccaactgg ccggcgtcgg
     2521 aaagctgctg ccggtgacgc agcggcgcgg gctgttcgca gcgatggccc gtgcaacgtt
     2581 tcgccggccc gggcgttgca cagccctgac cgcaaggctt gccgatcgcg cccggacgcg
     2641 ccatctggcc ggggcaccgg tccagggtct tgttctgtct ggagagcaca tgagcgtcgg
     2701 cctgctcgga ttgatcgacg acgtggtggg catcgccaag atcgccgcgg cctcgatcga
     2761 cgacgcggcg gggcaggcgg ccagggccgg cgcgaaggcc gccgggctcg tggtcgacga
     2821 caccgccgtg acgccgcgct tcctcgtcgg cttcgccgcc gagcgcgagc tgccgatcat
     2881 cgccaagatc accgtgggct cgctcaggaa caagctcgtc ttcctgctgc ccgccgccct
     2941 cctgctcagc ctcgtcgccc cctgggcgat cacgccgctc ttgatggtcg gcgggctcta
     3001 cctctcgttc gagggggcgg agaaggtgat ggaggcgctc ggctggcacc cgggcggtca
     3061 tggcagcgag gcagccgagg gcgacgacgc cgtgccggcg ccgctgctga gcgccgggga
     3121 ggaggcaagg cgggtcaagg gtgcgatccg caccgatttc atcctctccg cggagatcat
     3181 ggcgatcacg ctcgccaccg tcgccgcgag cccgttcgtc acccgggcga tcgtgctcgc
     3241 catcgtcggc gtcgtcatca cgctcgccgt ctacggggtg gtcgcgctga tcgtgaaggc
     3301 ggacgatgcc ggcctgttgc tggccgcccg cgacgggccg gtcagcggcc ccctcggccg
     3361 cggcctggtc cgcgtcatgc cccatttcct ccagctcctg agcttcgtcg gcaccgccgc
     3421 catgctctgg gtcggcggcg gcatcatcct gcacggcctc gaggagctgg gctggcgcac
     3481 cccggcaggc atcgtgcatc acgccggcga ggtcgcggcg caggcgctgg gggcgctcgg
     3541 cccgatcgcc ggctggctgg tcgccgccat cgcctcgggt gccctggcgc tcggcctcgg
     3601 tgcggtgacc gcggtggtga tgcaacgctt ggtgccagcg gccgtgcatt gagcgctcgc
     3661 cgggccgatc agaccggccg cgctgccggc ccatcgcatc gcggatcgag atcggccgcg
     3721 accggcgtga cggacgagac ggcgacgaag ggttcctgct ggtccgccgg aatgggtggc
     3781 cggcgccaga cgccataccc gacgaagacg gcgaagccga ccgccacgac gctctcgaag
     3841 gcgaagaagc tgtacggccc ccgcacggcc atgaccgccg aggcgagcag ggggccggcc
     3901 atggcaccta aggcatagat catcagaagc cggcccgagg cggagacata gtgcgcgcgg
     3961 tccaggcggt cgaacgcctg ggcgacgcag atcgggtaga cgctgctgat ggcgccgccg
     4021 aacaagacgg cgaggccgag caacgtcggc agcgacagcg ccgaaacact ggcgaacgcg
     4081 acgaacgccc aggcgacacc gaccatcgtc aggacggcgg cgaggacgat gcgccgatcg
     4141 aaccggtcgg ccagcatgcc gatcggccac tggaagccga agccgccgac caccaccatg
     4201 gtcatgaaca aggccgactg ggccacgtcg agcccgatcc cgcgggcgaa gacgacgccg
     4261 aggccgtaga acgatcccac cagcatgccg gccacgccgg cgccgacgac cccgaccggg
     4321 gaattggcat agagcgtgcg cacgctcagc cggcggtgcc cggcgagcgc cggtgcgccg
     4381 aggctggtca gcgccaccgg caccagcgcg agcgcgatga gcgccgctgc cagcatgaag
     4441 agctcgggcc cgtcgacatc ggccacgttg accagcacct gccccgacgc caccgccaga
     4501 tagaatgtca tcatgtagag gccgagcacc cgcccgcgcg tcgcgttgct gctgcgatcg
     4561 ttgagccagc tctcgatcgt cgcctgcagc ccggcgatgc agaagccgtt tatgaaccgg
     4621 agcgcgatcc aggccacggc cgtgaacagc atcggatagg ccagcgcggt cgtggccagc
     4681 accgccgcga aggcggcgaa agcgcggata tgcccgactc gcagaatgat cctcttgccg
     4741 tagttgccgc cgagcgccag gcccgcgtag taggcggcca tgaccaggcc gatcagcatc
     4801 accgggtatt cggccgcggc catgcgcagc ggcagcacga tgccgagcag gctgttgccg
     4861 acgatgaagg cgccgccgcc ggccagcaag gaccgcacgg ccgccagaac cttccccacg
     4921 ctcgttgtca tcgccgcttt ctcgactact cgggcagtgt gtagcgtgcg tccacgagct
     4981 tgcagatgat ctcgcgggtc tgccagggct cgatgtcgcc acggcgctcg atccgctccg
     5041 tctcggcggc gagaacctcg tccttgaccg catggcgaaa ccagcgggcg tagtcaccgc
     5101 ggcgcaggtg aaacatccac gtcgcttcgt cgatgccttg cgcgatctgg cagaagacga
     5161 cgaggttctg tgcccggagg ttgtggcggc cgtcggggcc gcggaaatag aagctgtgcc
     5221 agcgcagatt gccttcggcg tacttgcggt ggtggcgcag ccgctcggcc cggctcggct
     5281 gtgccgccat cgagaagggc ggctgcccgc cgtcgagaaa ccaggcgacc acacggccgg
     5341 gctcgtggtc gagacccgaa ggccaaatga aggcccggtc cgtcgcccgg gcaagctcgg
     5401 tcagggtctg atccggcgac cggccgatgg ccacggcgac atcgatcggc ctcaggatct
     5461 ccggggcgac gtgatcggga tgaaccgtca ccaggagcat ctcttgcagg cgttgcggca
     5521 gcacggctgc cgcatggccc catgtccgcg gcaacagatg gtgcgcctcg tccacgacga
     5581 t
//
LOCUS       DAOTFI010000284         5567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_17308_length_5567_cov_7.201560, whole genome shotgun sequence.
ACCESSION   DAOTFI010000284 DAOTFI010000000
VERSION     DAOTFI010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17308_length_5567_cov_7.201560"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5558_21115"
     CDS             complement(<1..220)
                     /locus_tag="P5558_21115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRY26883.1"
                     /translation="MTELSIRPEEIRDALDQFVQSYDPGAASREEVGRVTDAADGIAH
                     VEGLPSAMTNELLQFEDGTLGIALNLDVH"
     gene            complement(293..1105)
                     /locus_tag="P5558_21120"
     CDS             complement(293..1105)
                     /locus_tag="P5558_21120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRY26884.1"
                     /translation="MQGSSRAAAAVGQQALDAALTGAADPGRLGEDLFGMGATLDSSA
                     ALRRALTDPTRDADAKGDLAKRLFLGKVSNEALAVLMTLVGERWSTERDLTDTVERLA
                     VQSVLASAEAARTADAVEDELFRFERVVAGTPALRDALLDRQADPAAKAALVDRLLTA
                     KARPQTVRLARQAVLAPRGRRFAAVIEDYLEVIAQRRKQLTATVTSAIELTAAQRVRL
                     AGSLTAMYDRPVHLQTVVDPNVLGGIRVEIGDEVVDGTVLRKLDGARRHFGG"
     gene            complement(1105..1683)
                     /locus_tag="P5558_21125"
     CDS             complement(1105..1683)
                     /locus_tag="P5558_21125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRY26885.1"
                     /translation="MIPTLAASGEGHTPGWPTALPLLPQPAEMIVGLIAFGILYWLFK
                     TKVVPNVERMYAERTAAIEGGMAAAEKAQAEAKAALDQYTAQLQDARGEANEIREQAR
                     AQGAQIVAEMRAQAQAEASRIVDTAHKQIEAERQQAVIQLRGEVGRLSTDLAGRIVGE
                     SLQDEVRQKGIVERFLAELESGDIRPQKVGDA"
     gene            complement(1724..1933)
                     /gene="atpE"
                     /locus_tag="P5558_21130"
     CDS             complement(1724..1933)
                     /gene="atpE"
                     /locus_tag="P5558_21130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRY26886.1"
                     /translation="MTGSIAYLGYGLAAIGPGIGVGLIFAAYINGVARQPEARGMLQT
                     IAFTGMALTEALAILGLVFAFIFKG"
     gene            complement(2019..2858)
                     /gene="atpB"
                     /locus_tag="P5558_21135"
     CDS             complement(2019..2858)
                     /gene="atpB"
                     /locus_tag="P5558_21135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRY26887.1"
                     /translation="MAANVLGGLTVAGIPAEGGGEGETFQAPGTEVFYWPVIAGQDSS
                     WAITRPAVVLLISVGLIGWFFIRMSGRRLAVVPSKGQFVGEIAYDFVRNTIAKDIIGG
                     KDFIRFVPLLMTLFTVILVNNVFGIVPFVQYPTMSRIGFPIVLTLIVYLTYHAVGIKT
                     KHGIGTYLAGLIPPGLPGWLKPIMFVLEFLTYFVIRPLTLALRLFGNMLAGHLMLLVF
                     IVGGEYLLLHGDNIGLKGAGVLSFAFSIVMTFFELLVEFLQAFIFTLLSALYLSDAVS
                     EHH"
     gene            complement(3049..3501)
                     /locus_tag="P5558_21140"
     CDS             complement(3049..3501)
                     /locus_tag="P5558_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26888.1"
                     /translation="MTAPTPPHASEEAAAGRPFAIALRGAFVPSAVVGVLAVVVAAVV
                     RGPSAIVPALLGLIVGLGFFGSGLFLLSKLVRSANPLAFLAIGMAVYFGQVIVLLLFM
                     IAFYQAAWMDGQAFGGVVLAITIAWQVFLYRAWRRGRLPVYDEPEPQS"
     gene            complement(3544..4662)
                     /locus_tag="P5558_21145"
     CDS             complement(3544..4662)
                     /locus_tag="P5558_21145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRY26889.1"
                     /translation="MREYLLICVVAMTITYLSTPLARAVAVIVKAVTPLRDRDVHRVP
                     IPRLGGVALLLGFAAATLVARQLPFLKGVYSSGQMTGVLLGAAIICLVGALDDIRELD
                     WITKLAGQILAAGVMAYKGVVLLQVPFFGTSTVLPGPVLTLVTVLIVLVTVNAVNWID
                     GLDGLAAGIVAIASFAFFVYCYVVSQSYNPPNVFSTATFVTAATLGACLGFLPHNFNP
                     ARLFMGDSGALLLGLLLAAATISLTGNVDPNAVSGDATTAALLPIAAPLAVLSLPFVD
                     MVVAILRRARAGQKPWQADGKHLHHKMLHIGHGHRAAVLILYLWAALVAFGTVAFAFF
                     PPQQTLAALAAAACIAALLTAFLPTLTRARSRPSDEGL"
     gene            complement(4690..>5567)
                     /locus_tag="P5558_21150"
     CDS             complement(4690..>5567)
                     /locus_tag="P5558_21150"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRY26890.1"
                     /translation="GTKVSFSGKWFNAVHYGVHPETEDIDYDQVEALAKEHRPKVILA
                     GGSAIPRLIDFARFRAIADEIGAIFWVDAAHFIGLVAGKAIPSPVPHADVVSFTTHKV
                     LRGPRSGAIVCTEQHAAAIDKAVFPMMQGGPQMHTIAAKAVNFKECATPEYAAYAAQV
                     VRNAAVLAEALGDNGIRPTTGGTDTHLSLHDLQGVGVTGKDAEARAGAAGIVLNKNAI
                     PNDPAPPSIASGIRVGTPCVTTQGMGEDEMRRIAELITTAVVKGDADPDHAISREVRA
                     AVSDLVTRYPAYPRA"
BASE COUNT          860 a         1807 c         2091 g          809 t
ORIGIN      
        1 cgtggacgtc gaggttgagc gcgatgccca gggtgccgtc ctcgaactgg agcagctcgt
       61 tggtcatcgc cgagggcagg ccctcgacgt gcgcgatgcc gtcggcggcg tcggtgaccc
      121 ggccgacctc ctcgcggctg gccgcgcccg ggtcgtagga ctgcacgaac tggtccagcg
      181 cgtcgcggat ctcctccggg cggatcgaca gctcggtcat gtgcgttctc ttctcctcgg
      241 atcgtgcagt gagtcggtcg ttcgtgggta tgccgcccgg tcgggcgggc ggtcagccgc
      301 cgaagtgtcg tcgggcgccg tcgagcttgc gcagtaccgt gccgtcgacg acctcgtcgc
      361 cgatctcgac ccggatgccg ccgagcacgt tggggtcgac gacggtctgc aggtggacgg
      421 gccggtcgta catcgcggtc agcgagccgg ccagccggac ccgctgggct gcggtgagct
      481 cgatcgcgct ggtcaccgtc gcggtgagct gcttgcgccg ctgggcgatc acctcgaggt
      541 agtcctcgat gacggcggcg aagcggcgac cgcgaggggc gaggacggcc tggcgggcca
      601 gccgcacggt ctgcggacgc gccttggcag tcagcagccg gtcgacgagc gcagccttgg
      661 cggcggggtc ggcctgccgg tcgagcagtg cgtcgcgcag cgccggcgtg ccggccacga
      721 cccgctcgaa gcggaacagc tcgtcctcga ccgcgtcggc ggtccgggcc gcctcggcgc
      781 tcgcgagcac cgactgcacg gcgagccgct cgacggtgtc ggtgaggtcg cgctcggtcg
      841 accagcgctc gccgacgagg gtcatcagca cggcgagcgc ctcattgctc accttgccga
      901 ggaacagccg cttggccagg tcgcccttgg cgtccgcgtc gcgggtcggg tcggtgagcg
      961 cccggcgcag cgcggcgctc gagtcgagcg tcgcgcccat gccgaagagg tcctcgccga
     1021 gccggccggg gtcggccgcc ccagtgaggg cggcgtcgag ggcctgctgg ccgacggccg
     1081 ctgcggcgcg cgaggagcct tgcatcaggc gtcgcccacc ttctgcgggc ggatgtcgcc
     1141 ggactcgagc tcggccagga agcgctcgac gatgcccttc tggcggacct cgtcctgcag
     1201 cgactcgccg acgatccggc cggccaggtc ggtggagagc cggccgacct cgccgcgcag
     1261 ctggatgacg gcctgctggc gctcggcctc gatctgcttg tgcgcggtgt cgacgatccg
     1321 cgacgcctcg gcctgggcct gggcgcgcat ctcggcgacg atctgggccc cttgggcccg
     1381 cgcctgctcg cggatctcgt tggcctcgcc gcgggcgtcc tgcagctggg cggtgtactg
     1441 gtcgagcgcc gccttggcct cggcctgggc cttctcggcc gcggccatgc cgccctcgat
     1501 cgctgcggtg cgctcggcat acatccgctc gacgttcggc acgaccttgg tcttgaagag
     1561 ccaatagagg atgccgaagg cgatcaggcc gacgatcatc tcggccggct gcggcagcag
     1621 cggcagggcc gtgggccaac caggggtgtg gccctcgccg gatgccgcca gggtggggat
     1681 caccacgtcg cttccttacc tagtggggac ggggacgacg ggttcagccc ttgaagatga
     1741 aggcgaagac cagaccgagg atcgcgagcg cctcggtgag cgccatgccg gtgaaggcga
     1801 tggtctggag catgccgcgg gcctcgggct gacgggcgac accgttgatg taggccgcga
     1861 agatcaggcc gacaccgata ccggggccga tggcagccag accgtagccg aggtaggcga
     1921 tggagccggt cattgtggat tcctttctgg ggcaccgggg ctgtcccggt gtcgggtggt
     1981 cgtgcctgct gctggtcagt gcgggtggtg ctcgcgtgct agtgatgctc gctgactgcg
     2041 tcggagaggt agagcgcgga cagcaaggtg aagatgaagg cctggaggaa ctcgaccagg
     2101 agctcgaaga aggtcatcac gatcgagaag gcgaaggaca gcaccccggc gcccttgagg
     2161 ccgatgttgt cgccgtgcag cagcaggtac tcgccgccga cgatgaacac cagcagcatg
     2221 aggtggccgg cgagcatgtt gccgaagagt cgcagggcca gcgtcagcgg gcggatgacg
     2281 aagtaggtga ggaactcgag cacgaacatg atcggcttga gccagcccgg caggcccggg
     2341 gggatgaggc ccgcgaggta cgtgccgatg ccgtgcttgg tcttgatgcc gactgcgtgg
     2401 taggtgaggt agacgatcag ggtgagcacg atcgggaagc cgatccggct catcgtcggg
     2461 tactgcacga aggggacgat gccgaagacg ttgttgacga ggatcaccgt gaacagcgtc
     2521 atgagcagcg gcacgaagcg gatgaaatcc ttgccgccga tgatgtcctt ggcgatcgtg
     2581 ttgcggacga agtcgtaggc gatctcgccg acgaactggc ccttgctcgg cacgacggcc
     2641 aggcggcggc cggacatcct gatgaagaac cagccgatga gcccgaccga gatgagcagc
     2701 accacggcgg gccgggtgat cgcccagctg ctgtcctgac cggcgatgac cggccagtag
     2761 aagacctcgg tgccgggggc ctggaaggtc tcgccctcgc cgccgccctc ggccgggatc
     2821 ccagcgacgg tgagtccgcc gaggacgttc gcggccatcg cgtggaggct cacacctgct
     2881 ccttgtcgac tacggagtag accagctcga tctcgcgtgc tgacacctgg gccaccgcag
     2941 cgcactcagg gcgggcccgg ggcgcgtcgt ggtccttcag ggtcggctcg gagcctaccc
     3001 gaaccgaccg gctggtcggc acgcggcata cccgtgacga gacgcggctc acgactgcgg
     3061 ctccggctcg tcgtagaccg gcagccgtcc gcggcgccac gcgcggtaga ggaagacctg
     3121 ccaggcgatg gtgatcgcca gcacgacccc gccgaaggcc tgcccgtcca tccaggccgc
     3181 ctggtagaag gcgatcatga acagcagcag gacgatcacc tggccgaagt agaccgccat
     3241 cccaatggca aggaaggcaa ggggatttgc gctgcgcacg agcttgctga gcaggaacag
     3301 gccggagccg aagaagccga gcccaacgat gaggccaagc agcgccggga cgatggccga
     3361 cggcccgcgg accaccgcgg cgacgacgac ggcgaggaca ccgacgactg cgctgggcac
     3421 gaaggccccg cgcagggcga tggcgaaggg gcgccccgca gccgcctcct cactcgcgtg
     3481 agggggtgtc ggcgcggtca tcggcgaccc tttcggctcg gttcggaggg cttgtgaaac
     3541 ctatcacaag ccctcgtcac tgggtcgcga ccgagcccgc gtcaaagtcg gcaggaaggc
     3601 ggtgagcagg gccgcgatac aggcggccgc ggcgagcgcg gccagtgtct gctggggcgg
     3661 gaagaaggcg aaggcgaccg tgccgaaggc caccagggcc gcccacaggt agaggatgag
     3721 gaccgccgcg cgatggccgt gaccgatgtg cagcatcttg tggtgcaggt gtttcccgtc
     3781 ggcctgccag ggcttctgcc cggcccgcgc gcggcgcagg atcgcgacga ccatgtcgac
     3841 gaagggcagc gagagcaccg ccagcggtgc ggcgatgggc agcagggccg ccgtcgtcgc
     3901 gtcgccgctg accgcattgg ggtcgacgtt gccggtgagc gagatcgtcg ccgccgcgag
     3961 cagcaggccg agcagcaggg cgccggagtc gcccatgaac agccgagccg ggttgaagtt
     4021 gtgcggcagg aaacccaggc aggcgcccag ggtcgccgcg gtgacgaagg tcgcggtgct
     4081 gaagacgttg ggcgggttgt agctctgact cacgacgtag cagtagacga agaaggcgaa
     4141 cgaggcgatc gcgacgatcc ctgcggccag cccgtcgagc ccgtcgatcc agttcaccgc
     4201 attgaccgtg acgagcacga tgagaacggt caccaaggtc agcaccggcc cgggcagcac
     4261 ggtgctcgtg ccgaagaacg gcacctgcag cagtacgacg cccttgtagg ccatcacccc
     4321 ggcggccagg atctgcccgg cgagcttggt gatccagtcg agctcgcgga tgtcgtcgag
     4381 ggccccgacc agacagatga tggccgcgcc gagcagcacg ccggtcatct gcccgctgga
     4441 gtagacgccc ttgaggaagg gcagctgccg ggcgacgagc gtcgcggccg cgaagccgag
     4501 cagcagggca actcccccga ggcgcgggat gggcacccgg tgcacgtccc ggtcgcgcag
     4561 cggcgtgacc gccttgacga tgacggcgac cgcgcgggcc aggggcgtgg agagatacgt
     4621 gatggtcatc gccaccacgc agatgagcag gtactcccgc acgctgccgc gcctcgcgct
     4681 cgccggcgac tacgcccgcg ggtatgccgg gtagcgcgtc acgaggtcgc tcacggccgc
     4741 gcgcacctcg cgcgagatgg cgtggtcggg gtcggcgtcg cccttgacga ccgccgtcgt
     4801 gatgagctcg gcgatccggc gcatctcgtc ctcgcccatc ccctgggtgg tgacgcacgg
     4861 cgtgccgacc cggatgccgg aggcgatgct cggcggggcc gggtcgttgg ggatggcgtt
     4921 cttgttgagg acgatgccgg ctgcaccggc gcgcgcctcg gcgtccttgc cggtgacccc
     4981 gacgccctgc aggtcatgca gcgacaggtg ggtgtcggtg ccgccggtgg tcggacggat
     5041 cccgttgtcc cccagcgctt ctgcgaggac ggcggcgttg cgcaccacct gcgcggcata
     5101 cgcggcatac tccggcgtcg cgcactcctt gaagttgacg gccttggcgg cgatggtgtg
     5161 catctgcggg ccgccctgca tcatcgggaa gaccgccttg tcgatcgcgg cggcatgctg
     5221 ctcggtgcac acgatggcgc cggagcgcgg gccgcgcagc accttgtggg tcgtgaagga
     5281 gacgacgtcg gcgtgcggca ccgggcttgg gatcgccttg ccggcgacca ggccgatgaa
     5341 gtgtgcggcg tcgacccaga agatcgcgcc gatctcgtcg gcgatggccc ggaagcgggc
     5401 gaagtcgatg agccgcggga tcgccgagcc accggcgagg atgaccttgg ggcggtgctc
     5461 cttggccagc gcctcgacct ggtcgtagtc gatgtcctcg gtctcggggt ggacgccgta
     5521 gtgcaccgcg ttgaaccact tgccggagaa ggagaccttg gtgccgt
//
LOCUS       DAOTFI010000285         5491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_17603_length_5491_cov_5.111663, whole genome shotgun sequence.
ACCESSION   DAOTFI010000285 DAOTFI010000000
VERSION     DAOTFI010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17603_length_5491_cov_5.111663"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="P5558_21155"
     CDS             <1..512
                     /locus_tag="P5558_21155"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRY26891.1"
                     /translation="PDYRVIEEGLPGRTTVHADPIEGAHMNGLAALPMLLASHSPIDL
                     MTIKLGTNDLKVRHSVCAADIARSIELLVLTTRQLCTAPGRTVPKILIIAPPPIEEAG
                     CLAEIFAGGRAKSLEVGPWLRRSAEFLGTGFLDAGDHIEVSPIDGIHYDEAAHATLGR
                     AIAAAVRAL"
     gene            543..1796
                     /locus_tag="P5558_21160"
     CDS             543..1796
                     /locus_tag="P5558_21160"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRY26892.1"
                     /translation="MFHDAKARLDSAAATLGVTAEVVERLRYPDETLCASLPIRLDDG
                     SIRVLKGFRCRYNEVRGPTKGGIRFHPSVGIDEVMALAFWMTIKTAVVDLPFGGAKGG
                     VAVDAKGLSPRELEGVARAYVRAFAEMIDEDRDIPAPDMYTGGIVIAWMTDEITALER
                     RPMPAAITGKPRQLGGSLGRDTATGDGAFLCLERLADRLGLGQGASIAVQGFGNAARV
                     FARRAAEAGYRVTAVSDSSGAAHDPGGLDLDALASHKNETGSVEGFAGALDRDALLTT
                     ECDLLVPAALGGVIDADLARKLRCKAILEIANGPVQPAADAVLEECRIDVVPDILANA
                     GGVIVSYFEWVQNRTGDYWAAGTVAERLAERLRTSTDAVVERAERHACGLRQGAYLLA
                     LERLCAAIAAKGTQAFYNGGGAGDA"
     gene            1793..3184
                     /gene="thrC"
                     /locus_tag="P5558_21165"
     CDS             1793..3184
                     /gene="thrC"
                     /locus_tag="P5558_21165"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRY26893.1"
                     /translation="MTTYTSTRGGSHGRSFEDVLLRGLALDGGLFVPETWPVLDLEAL
                     AGLDYPTTVARLLAPFTTGCFDAAELEAMARDAYAAFRHPATAPLVQLDHDEWLLELF
                     HGPTFAFKDFAMQLLGRMFEAVLKRRGESITIVGATSGDTGAAAVRAFAGLERIRVAI
                     LFPEGRISPVQQRQMTTASAANVEIIAVQGTFDDCQNRLKDMFNDQAFRGDLKLAAVN
                     SINWARVVAQAGYYVYSALRLGGLARPLAFAVPTGNFGDVYAGYVAHRIGLPVARLIV
                     ATNTNDILARFLSSGRYARGEVAATMSPSMDIQVASNFERLLFELLDRDGAAVAQLMA
                     DFARDGALELDENRLAAARRLFAGARVDEATTAATIAEVRAATGQLVDPHTAVGIRAG
                     RDLCPPGTPLVTLATAHPAKFPEAMTRATGEAAPLPEPLAAMLQAHERFTTLPNDLAA
                     LQAHLRAWAGPTA"
     gene            3202..4458
                     /locus_tag="P5558_21170"
     CDS             3202..4458
                     /locus_tag="P5558_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY26894.1"
                     /translation="MQITTLSNGIRVVTETMPRVETASFGIWVDAGARYEAPEVNGVA
                     HMLEHMAFKGTATRTAKAIAEEIEDVGGSLNAYTGREHTAYYARILAEDVPLAADILT
                     DILRHSTFDAEEYEKERHVILQEIGETQDTPSDLVFDQFQEAAFPNQPIGRSILGSEA
                     IIETLPRDALQTFIDSHYGPEALIVAASGKVEHDAIVALATEKFGDLAKSPKPVPAPA
                     SYTGSEWLSGRDLEQVHLCLGLEAFAYTDPDFIPLQVFQTALGGGMSSRLFQEVRENR
                     GLCYSIFTFAGLHKDSGVFGIYAGTGEQDVPELVHVIAKELRDLASSIGEAELRRAKA
                     QLKASLLMGQEGCFSVCEDIARQFLCFGDYLTPAELTARYEAVDAAAIARVAARLLKD
                     PKPTVAAIGPAKGLPDAGTWLDRLSH"
     gene            4458..>5491
                     /locus_tag="P5558_21175"
     CDS             4458..>5491
                     /locus_tag="P5558_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY26895.1"
                     /translation="MRITSIRETAAPISSEIANAYIDFSKMTCSLVAVTTDLVRDGRP
                     VTGFGFSSNGRYAAGAIMRDRMIPRLLEAAPDTLLDPANGLLDPFRCWDRMMTNEKPG
                     GHGERSVAVGTLDMAIWDALAKIEGVPLWRLLADRYKGGVADDSVWVYAAGGYYHPGK
                     GIDGLVDEVKSYRDMGYVDFKLKIGGAPLPEDLKRIEAVIGVAGSGAHVAVDANGRFD
                     QATAIAYAKALEPLGLLWYEEPGDPLDYALQADLAPHYRHAMATGENLFSTIDATNLV
                     RYAGMRPDRDWLQFDCALSYGLCEYTRTLAMLDAHGWSSRRVVPHGGHQMSLNIAAGL
                     HLGGNESYPG"
BASE COUNT          825 a         2178 c         1703 g          785 t
ORIGIN      
        1 gccccgacta ccgcgtcatc gaggagggcc tgcccggccg caccacggtc catgcggacc
       61 cgatcgaggg tgcgcacatg aacgggctgg ccgccctgcc gatgctgctc gccagccatt
      121 cgcccatcga cctcatgacc atcaagctcg gcaccaacga tctgaaggtg cgccactcgg
      181 tctgtgcggc cgacatcgcc cgctcgatcg agctcctcgt cctgaccacc cgccagctct
      241 gcaccgcccc cggccggacc gtgccaaaga tcctgatcat cgccccgccg ccgatcgagg
      301 aggccggctg cctcgccgag atcttcgccg gcggcagggc gaagtcgctg gaggtcggtc
      361 cctggctccg ccgctccgcc gaatttctcg gcacgggctt tctcgacgcc ggcgaccata
      421 tcgaggtaag cccgatcgac gggatccact acgacgaggc ggcgcacgcc accctcggcc
      481 gggcgatcgc cgcggccgtg cgggcgctct gagcgccggc cgccagcaga acgaggccac
      541 gaatgttcca tgacgccaag gcgcggctgg attccgccgc ggcgacgctc ggcgtgaccg
      601 ccgaggtcgt cgagcggctg cgctacccgg acgagaccct gtgcgccagc ctgccgatcc
      661 gcctcgacga cggctcgatc cgcgtgctca agggctttcg ctgccgctac aacgaggtgc
      721 gcggcccgac caagggcggc atccgcttcc atcccagcgt cggcatcgac gaggtgatgg
      781 cgctggcctt ctggatgacc atcaagacgg cggtggtcga tctgccgttc ggcggcgcga
      841 agggcggggt cgcggtcgac gccaagggcc tgagcccgcg cgagctcgag ggcgtggcgc
      901 gcgcctacgt ccgcgccttc gccgagatga tcgacgagga ccgcgatatc ccggcgcccg
      961 acatgtacac gggcggcatc gtcatcgcct ggatgaccga cgagatcacc gccctcgagc
     1021 gccggccgat gccggccgcc atcaccggca agccgcgcca gctcggcggc tcgctcggcc
     1081 gcgacaccgc gaccggcgac ggcgcgttcc tgtgcctgga gcgcctcgcc gaccgtctcg
     1141 gcctcgggca gggggcgagc atcgccgtcc agggcttcgg caacgccgcg cgcgtcttcg
     1201 cccgtcgcgc ggccgaggcc ggctatcgcg tcaccgccgt ctccgacagc agcggtgccg
     1261 cgcacgaccc gggtgggctc gatctggacg cgctggccag ccacaagaac gagaccggct
     1321 cggtcgaagg cttcgccggc gcactcgatc gcgatgccct gctgaccacc gaatgcgatc
     1381 tcctggtgcc cgcggctctg ggcggcgtga tcgatgcaga cctcgcccgg aagcttcgct
     1441 gcaaggccat cctggagatc gccaacggcc ccgtgcagcc ggccgccgac gcggtgctcg
     1501 aggagtgtcg catcgatgtc gtccccgaca tcctcgccaa tgccggcggc gtgatcgtct
     1561 cctacttcga gtgggtgcag aaccggaccg gggactactg ggccgccggg accgtggccg
     1621 agcgcctggc ggagcgtctg cgcaccagca ccgacgccgt cgtcgagcgt gccgagcgcc
     1681 atgcttgcgg cttgcggcag ggcgcctatc tcctggccct cgagcggctg tgcgcggcca
     1741 tcgccgccaa gggcacgcag gccttctaca atggcggcgg ggcaggggac gcatgaccac
     1801 ctacaccagc acgcgcggcg gcagccacgg ccggagcttc gaggacgtgc tgctgcgcgg
     1861 cctcgccctg gatggcgggc tcttcgtccc cgagacctgg ccggtcctcg acctcgaggc
     1921 cctggccggc ctcgactacc cgaccaccgt ggcgcgcctc ctcgccccct tcaccaccgg
     1981 ctgcttcgac gcggccgagc tcgaagccat ggcccgggac gcctatgccg ccttccgcca
     2041 ccccgccacg gcccccttgg tccagctcga ccacgacgag tggctgctcg agctcttcca
     2101 cggcccgacc ttcgccttca aggacttcgc catgcagctt ctcggccgca tgttcgaggc
     2161 ggtcctgaag cggcgagggg agagcatcac catcgtcggc gccacctcgg gcgacaccgg
     2221 tgcggccgcc gtgcgcgcct tcgccgggct ggagcgcatc cgggtcgcca tcctcttccc
     2281 cgaaggccgc atctcgcccg tccagcagcg ccagatgacc acggcatcgg cggccaatgt
     2341 cgagatcatc gccgtccagg gcacgttcga cgactgccag aaccgcctca aggacatgtt
     2401 caacgaccag gcgttccgcg gcgacctcaa gctcgccgcg gtcaactcga tcaactgggc
     2461 ccgcgtcgtg gcccaggccg gctactacgt ctattccgcg ctccgcctcg gcggcctcgc
     2521 ccggcccttg gccttcgccg tgccgaccgg caatttcggc gacgtctatg ccggctacgt
     2581 cgcccatcgc atcggcctgc cggtggcgag gctgatcgtc gccaccaaca ccaacgacat
     2641 cctcgcccgc ttcctctcct ccggccgcta tgccaggggc gaggtcgccg ccaccatgag
     2701 cccgtccatg gacatccagg tggcctccaa cttcgagcgc ctgctcttcg agctcctcga
     2761 ccgcgacggc gccgccgtgg cgcagctcat ggccgacttc gcccgcgacg gagcgctcga
     2821 gctcgacgag aaccgcctcg ccgccgcccg ccgcctgttc gccggtgccc gggtcgacga
     2881 ggcgacgacg gccgccacca tcgccgaggt ccgcgccgcc accggccagc tcgtcgatcc
     2941 gcacaccgcc gtcggcatcc gcgccggccg cgacctctgc ccgccaggga cgccgctcgt
     3001 caccctcgcc acagcccatc cggcgaagtt cccggaagcc atgacccggg ccaccggcga
     3061 ggcggcgcct ttgccggagc cgctcgccgc catgctgcag gcccatgagc gcttcaccac
     3121 cctgccgaac gatctggccg ccctgcaggc gcatctccgc gcctgggccg gccccaccgc
     3181 ctgaagcgaa ccagagaaaa catgcagatc accacgcttt cgaacggcat ccgcgtcgtc
     3241 accgagacca tgccccgggt cgagaccgcg agcttcggca tctgggtcga tgccggcgcc
     3301 cgctacgagg cgcccgaggt caacggcgtc gcccacatgc tggagcacat ggccttcaag
     3361 ggcaccgcca cccgcaccgc caaggcgatc gccgaggaga tcgaggacgt cggcggctcg
     3421 ctcaacgcct atacgggccg cgagcacacc gcctactacg cccgcatcct cgccgaggac
     3481 gtgccgctcg ccgccgacat cctgaccgac atcctccgcc actcgacctt cgatgccgag
     3541 gagtacgaga aggagcgcca cgtcatcctg caggagatcg gcgagaccca ggacaccccc
     3601 tccgacctcg tcttcgacca gttccaggag gcggccttcc cgaaccagcc gatcggccgc
     3661 tcgatcctgg gctccgaggc gatcatcgag accctgccgc gcgacgcgct ccagaccttc
     3721 atcgacagcc actacggccc cgaggcgctg atcgtcgccg cctccggcaa ggtcgagcac
     3781 gacgccatcg tcgccctcgc caccgagaaa ttcggtgatc tggcgaagag ccccaagccc
     3841 gtcccggcac ccgccagcta caccggcagc gagtggctct ccggccgcga cctcgagcag
     3901 gtccacctct gcctcggcct cgaggccttc gcctacaccg accccgactt catcccgctc
     3961 caggtcttcc agaccgccct cggcggcggc atgtcctcgc gcctcttcca ggaggtccgc
     4021 gagaaccgcg gattgtgcta ctccatcttc accttcgccg gcctgcacaa ggattccggc
     4081 gtcttcggca tctatgccgg caccggcgag caggacgtgc ccgagctcgt ccatgtcatc
     4141 gcgaaggagc ttcgggacct cgcctccagc atcggcgagg ccgagctccg ccgtgcgaag
     4201 gcccagctca aggcgagcct gctcatgggc caggaaggct gcttctccgt gtgcgaggac
     4261 atcgcccgcc agttcctctg cttcggcgac tacctcaccc cggccgagct caccgcccgc
     4321 tacgaagccg tcgacgcagc cgccatcgcc cgcgtcgccg cccgcctgct gaaggacccc
     4381 aagcccaccg tcgccgccat cggcccggcc aagggcctgc cggacgcggg gacgtggctc
     4441 gaccgtctgt cgcactgatg cgcatcacgt ccatccgcga gacggccgca ccgatcagct
     4501 ccgagatcgc caacgcctat atcgacttct cgaagatgac ctgctcgctg gtcgccgtca
     4561 ccaccgacct cgtccgcgac ggccggccgg tcaccggctt cggcttcagc tccaacggcc
     4621 gctacgctgc cggggccatc atgcgcgacc gcatgatccc gcgcctgctc gaggccgcgc
     4681 ccgacaccct cctcgacccc gcgaacggcc tcctcgatcc cttccgctgc tgggatcgca
     4741 tgatgaccaa cgagaagccc ggcggccacg gcgagcgctc ggtcgccgtc ggcacgctcg
     4801 acatggccat ctgggatgcg ctcgccaaga tcgagggcgt ccccctctgg cgcctgctcg
     4861 ccgaccgcta caagggcggc gtcgccgacg actccgtctg ggtctacgcc gccggcggct
     4921 actaccatcc gggcaagggc atcgacggcc tggtcgacga ggtgaagtcc taccgggaca
     4981 tgggctatgt cgacttcaag ctgaagatcg gcggcgcccc tttgcctgag gacctgaagc
     5041 gcatcgaggc cgtgatcggc gtcgccggca gcggcgccca tgtcgctgtc gacgccaacg
     5101 gccgcttcga ccaggccacc gccatcgcct acgcgaaggc cctcgagccc ctgggcctcc
     5161 tctggtacga ggagcccggc gacccgctcg actacgcgct ccaggccgac ctcgcccccc
     5221 actaccggca tgcgatggcc accggcgaga acctcttctc caccatcgac gccaccaacc
     5281 tcgtccgcta cgccggcatg cgccccgatc gcgactggct gcaattcgac tgcgccctca
     5341 gctacggcct ctgcgagtac acccgcaccc tcgccatgct cgacgcgcac ggctggtcca
     5401 gccgccgcgt cgtgccgcat ggcggccacc agatgagcct caatatcgcc gccggcctgc
     5461 atctcggcgg caacgagagc taccccggca t
//
LOCUS       DAOTFI010000286         5468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_17699_length_5468_cov_6.992980, whole genome shotgun sequence.
ACCESSION   DAOTFI010000286 DAOTFI010000000
VERSION     DAOTFI010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17699_length_5468_cov_6.992980"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="P5558_21180"
     CDS             <1..194
                     /locus_tag="P5558_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY26896.1"
                     /translation="GFHEGFGNLYRDFAEQVAARLEGRDADPLAGTIPTAEDGLDTLL
                     FIDACIRSSETGSWQVIKA"
     gene            complement(184..942)
                     /locus_tag="P5558_21185"
     CDS             complement(184..942)
                     /locus_tag="P5558_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HRY26897.1"
                     /translation="MAQQARTAELYRMVMKDHLCPFGLKAKSLLERKGFTVEDHPLRS
                     HAETEAFQREHDVETTPQVFIGGERVGGYDDLREYFGQEVKKEGETSYQPVIAIFATA
                     FLMALAVSWAAFDTVLTVRALEWFIAISMCFLGVQKLQDLESFSTMFLNYDLLARREV
                     RYAYLYPFGETLAGVLMIAGALIWLAAPVALFIGTVGAVSVFKAVYVDKRELKCACVG
                     GSSNVPLGFVSLTENVMMVAMAIWMVARSWWLMP"
     gene            complement(974..1456)
                     /locus_tag="P5558_21190"
     CDS             complement(974..1456)
                     /locus_tag="P5558_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HRY26898.1"
                     /translation="MSNSYGRFLAMIATSTIVMFGLMYLNTYALDHIFFSETRAWMAL
                     YMGATMGIIMLLFMLRMYGSTAANIAILAGCAVLFAVALWLVRSQATIDDVSYMRAMI
                     PHHSIAILTSERAGISDPRVRELADGIIEAQRLEIAEMKALIADLEGGPPATPELDGK
                     "
     gene            2077..3063
                     /locus_tag="P5558_21195"
     CDS             2077..3063
                     /locus_tag="P5558_21195"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HRY26899.1"
                     /translation="MSTPPLDVTNLWARTGDSRQLASLRRVTLEDGPEAGVRALAFDT
                     GGGLAFWALEGRGLDLATLSWRGLNCAWQGPGGFARPGLHPAESDQGQGFRRAYSGLL
                     VTCGLGHTRQPRGGYPLHGRLPFTPARLIAAGEDWHAPEPFLYAEAEVVDAIAGGESW
                     LLRRRIEAPTGGSTIRLTDTVTNRSRETRPQPILYHFNLGYPLLRPGCRFDCNGTLLL
                     RPPAEPDPGAKPRVTCRPAPSDAGWARCSLTAPPIDDWPGIRLELAFATDTLPFLQVL
                     EDFRPGMNLIGVEPANTDRSPEGLSVLDPSADLAAGASRQYRLTLTLGTMTA"
     gene            3169..4461
                     /locus_tag="P5558_21200"
     CDS             3169..4461
                     /locus_tag="P5558_21200"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY26900.1"
                     /translation="MSIITATMPSPTSTPIDVPELPAAMLPPFLFSARMPPLPVAGAL
                     RRGQASWPAIAAEIGAGYLARGLVGSFEAQAFGAAPGDLDALIAVPARNEAGSIRAVL
                     TGLRRALAASPVRAAIVLFANNCDDGTAGNVAELAPALGIPLVLVEGRLRRPHAHVGL
                     ARRIAMDIALGMGTPDCAILTTDADTRVGDEWVRELTAPLAEGFELVCGDFEIEDPGP
                     AVRGLQLHPLWRMEARYAALQNRVQHACDQVTGRQPVGGPCPHYVEAGASLGITARLY
                     RRLGGLPPVACSEDRALVRAAELAGARICYAERAPVRTSGRLDGRAAGGLAATLSRRL
                     VAAAPTLDQRLRPATGLRRLWTHALLAAAGHERAALDWPSATPAALHARMENEWQRSV
                     VEPPMPARVVRRELPRLARLLRLEIEPALARWAAGHGR"
     gene            4458..>5468
                     /locus_tag="P5558_21205"
     CDS             4458..>5468
                     /locus_tag="P5558_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRY26901.1"
                     /translation="MTVSALRQPPEPALSFDADLERCADAIRQLSPPAEQECPAAPMV
                     AELRRHGLLAAAAPAADGGLGLAHEPEDPLRLVRMLTLIGRANLSAGRLLEGHVNALK
                     LVAIHAEGEARSRLLGVARSGGLFGVWGAEGPHPVRLAPTGDGRLELTGEKLFASGAD
                     TVDVAVVTAKSTDGTTLLVALPTATLAGRLFPGEWSVSGMKATASGRCNLDGVCIDPN
                     DLLGGPDAYFREPWFEGGVWRYAAVQLGGMYAIAGHAADQLRARRQATAPLQAARLRR
                     MVTACETARLWVASAALATEAPAAQPATARVAVLARLEVAEAAVRLLQLADEAMGAAS
                     FAL"
BASE COUNT          778 a         2040 c         1846 g          804 t
ORIGIN      
        1 agggcttcca cgagggcttc ggcaatctct accgcgactt cgccgagcag gtggcagccc
       61 ggctcgaagg ccgcgacgcg gacccgctcg ccggcacgat ccccacggcc gaggacggcc
      121 tcgacacgct tctcttcatt gacgcctgca tccgctcgtc cgagacgggc agctggcagg
      181 tcatcaaggc atgagccacc agctcctggc caccatccag atcgccatcg ccaccatcat
      241 gacgttctcg gtgagcgaga cgaagcccag cggcacgttg ctgctgccgc cgacgcaggc
      301 gcatttgagc tcgcgcttgt cgacatagac cgccttgaag accgagacgg ccccgaccgt
      361 gccgatgaag agggccacgg gggcggcgag ccagatcagg gcaccggcga tcatcagcac
      421 acccgccagc gtctcgccga aggggtagag ataggcgtag cgcacctcgc gccgggcgag
      481 gaggtcgtag ttgaggaaca tcgtgctgaa gctctcgaga tcctgcagct tctgcacgcc
      541 gaggaagcac atcgagatgg cgatgaacca ctcgagcgcc cgcacggtga ggaccgtgtc
      601 gaaggccgcc cagctcacgg ccagcgccat caggaaggcc gtggcgaaga tggcgatgac
      661 gggctggtag ctcgtctcgc cctccttctt cacctcctgg ccgaaatatt cccgcaggtc
      721 gtcgtagccg ccgacccgct cgccgccgat gaagacctgc ggcgtcgtct cgacatcgtg
      781 ctcgcgctgg aacgcctcgg tctccgcgtg cgaccgcaag gggtgatcct cgacggtgaa
      841 gcccttgcgc tcgagcagcg acttcgcctt cagcccgaag gggcacaagt ggtccttcat
      901 caccatccga tagagctcgg ccgttcgtgc ctgctgtgcc atcaaaaggc cctccttctc
      961 ggttgaggca cgctcatttc ccatcgagct cgggagtcgc cggcggcccg ccctcgaggt
     1021 cggcgatgag cgccttcatc tcggcgatct cgagccgctg cgcctcgatg atgccgtccg
     1081 ccagctcgcg cacccgcggg tcgctgatgc cggcccgctc gctcgtcagg atggcgatcg
     1141 aatggtgcgg gatcatcgcc cgcatgtacg acacgtcgtc gatggtcgcc tggctgcgga
     1201 cgagccagag cgccacggcg aagagcacgg cgcagcccgc caggatggcg atgttggcgg
     1261 ccgtgctgcc gtacatcctg agcatgaaga ggagcatgat gatccccatg gtcgccccca
     1321 tgtagagggc catccaggcg cgcgtctcgc tgaagaagat gtgatcgagc gcataggtgt
     1381 tcaggtacat gaggccgaac atcacgatgg tcgacgtcgc gatcatcgcc aggaatcggc
     1441 cgtaggagtt gctcatgctt ggctccctcg atggtcaccg ggtcgcgcaa caccgggcaa
     1501 ggccggcggg cggcgcggga cgggttcgat ggccaaacaa ccttccaccg ttggaaggtt
     1561 cccttttttc tggatgcccc tccccgatgc cccgcgcatc atcacgaatc gttatgaccg
     1621 gcgccgcccg gatcgccggc catcgccgcc gggccggcaa acgaccccac cgacccggcg
     1681 aatgtcgcgg aatccgcgac atcacccctt gggttgtcgc gttataagcg acaggcaagg
     1741 cccgattgtc gcgtattccg cgacataagc gttcgtgatg tcgcggattc tgcgacatcc
     1801 gacccgaaac gtcgcggatt ttgcgacagc acccgacgaa cgaaccggag gattcgacga
     1861 acgaacccgg cgaaaccgcc gttccgcggg ttcgttcgtc gctccggcgg gttcgttcgc
     1921 accccccggg ccgccggatt cctgcttttc cgcccaggtc cgatcccgcc acccctgttt
     1981 tgcccaacgg acccgaggcg gcgcttgccc cgacgccctt tctaggctat aaatcccaga
     2041 acgaggccgt gcctcggcgt cgccaggagc cacggcatga gcacgccccc actggacgtc
     2101 accaacctct gggcgcgcac cggcgatagc cgccagctcg cctcgctccg ccgtgtcacg
     2161 ctcgaggacg gaccggaagc cggggtccgc gccctcgcct tcgacaccgg cggcgggctc
     2221 gccttctggg cgctcgaagg gcgcggcctc gatctggcga cgctctcctg gcgcggcctc
     2281 aactgcgcct ggcaggggcc cggcggcttc gcccggccgg ggctgcatcc ggccgagagc
     2341 gaccaggggc agggcttccg gcgggcctat tccggcctgc tcgtcacctg cggcctcggc
     2401 catacccgcc agcccagggg cggctacccg ctgcatggcc gcctgccctt cacccccgcc
     2461 cggctgatcg cggcgggcga ggactggcat gcgcccgagc ccttcctcta tgccgaggcg
     2521 gaggtggtcg acgccatcgc cggcggcgag agctggctgc tgcgccgccg gatcgaggcg
     2581 ccgaccggcg gcagcaccat ccgcctcacc gacaccgtca ccaaccgctc gcgcgagacc
     2641 cggccgcagc cgatcctcta ccacttcaac ctcggctacc cgctgctccg gcccggctgc
     2701 cgcttcgact gcaacggcac cttgctgctc cgcccgccgg ccgagcccga cccgggcgcc
     2761 aagccccgcg tcacctgccg cccggcgcca tcggacgcgg gctgggcacg ctgcagcctc
     2821 acagccccgc ccatcgacga ctggcccggc atccgcctcg agctggcctt cgccaccgac
     2881 accctgccct tcctgcaggt cctcgaggat ttccggccgg gcatgaacct gatcggcgtc
     2941 gagccggcca acaccgaccg ctcgcccgaa ggcctgagcg tcttggaccc gagcgccgac
     3001 ctcgccgccg gtgccagccg tcagtaccgg ctcaccctga ccctcgggac gatgaccgcc
     3061 taggcgccgc ctgccgggca ccccggggaa cgcccgtgga accgattccg acagcaaagc
     3121 gttcaggagc gccccaacgc tcccgtgctt caggtcggac accgctcgat gtcgatcatc
     3181 accgccacca tgccgagccc gacgtcgacg ccgatcgacg tgcccgagct tccggcggca
     3241 atgttgccgc cgtttctttt cagtgcccgc atgccgccct tgccggtcgc cggcgccttg
     3301 cgacgcggcc aggcgagctg gccggcgatc gccgccgaga tcggcgccgg ctatctggcg
     3361 aggggcctgg tcggctcgtt cgaggcgcag gcctttggcg ctgctccggg ggacctggac
     3421 gcgctgatcg ccgttccggc gaggaacgag gcgggcagca tccgcgcggt cttgaccggt
     3481 ctccggcggg ccctggccgc gagcccggtg cgcgccgcca tcgtcctctt cgccaacaat
     3541 tgcgacgacg gcacggcggg caacgtcgcc gagctggcgc cggcgctcgg catccccctt
     3601 gtcctggtgg aagggcggct tcgccggccg catgcccatg tcggcctcgc ccggcgcatc
     3661 gccatggaca tcgccctggg aatgggcacg ccggactgcg ccatcctgac gaccgatgcc
     3721 gacaccaggg tcggcgacga atgggtgcgc gagctcaccg caccgctcgc ggaaggcttc
     3781 gagctcgtct gcggcgattt cgagatcgag gacccggggc cggcggtgcg tggcctgcag
     3841 cttcacccgc tctggcggat ggaggcccgc tatgcggcgc tgcagaaccg ggtgcagcat
     3901 gcctgcgacc aggtgaccgg ccggcagccg gtcggcggcc cctgtcccca ttacgtcgag
     3961 gcaggcgcca gtctcgggat caccgcaagg ctctatcggc ggctcggtgg cctgccgccg
     4021 gtcgcctgca gcgaggaccg ggccctggtc cgcgcggcgg agctggccgg cgcccggatc
     4081 tgctatgccg aacgggcacc ggtgcggacc tccggccggc tcgatggccg ggccgccggc
     4141 gggctggcgg cgaccttgag ccgtcggctg gtcgccgcgg cacccaccct ggaccagcgg
     4201 ctgcgcccgg ccaccggcct gcgccggctc tggacccatg cgctcctggc cgctgccggc
     4261 cacgagcgcg cggcgctcga ttggccgagc gcgacgccgg ccgcgcttca cgcccgcatg
     4321 gagaacgaat ggcagcggtc ggtggtcgag ccgccgatgc cggcgagagt cgtgcgccgg
     4381 gagcttcccc gactggcccg gctgctcagg ctcgagatcg agccggcgct ggcgcgctgg
     4441 gcggcggggc acgggcgatg acggtcagcg ccctgcggca accgcccgag cccgctttgt
     4501 cattcgacgc cgatctggaa cgctgcgccg acgccatccg gcagctatcg ccaccggcgg
     4561 agcaggaatg cccggcggca cccatggtcg ccgagcttcg ccgccacggc ctgctcgccg
     4621 cggcggcgcc ggccgctgat ggcggcctcg gcctggcgca cgagccggag gacccgctgc
     4681 gactcgtgcg gatgctgacc ctgatcggcc gcgccaatct ctccgccggg cgcctcctcg
     4741 agggccatgt gaacgcgctc aagctcgtgg cgatacatgc cgagggcgag gcccggagca
     4801 ggctgctcgg cgtggcgcgc tccggcggcc tcttcggcgt ctggggcgcc gaggggccgc
     4861 accccgtccg attggcgcca accggggatg gccggctgga gctgaccggc gagaagctct
     4921 tcgcctccgg cgccgacacc gtcgacgtcg ccgtcgtgac ggcgaagagc acggacggca
     4981 cgaccctgct ggtcgccctg ccgaccgcga cgctcgccgg gcggctcttc ccgggcgaat
     5041 ggtcggtttc gggcatgaag gccacggcct ctggacgctg caacctggac ggcgtttgca
     5101 tcgacccgaa cgacctgctc ggcgggccgg acgcctattt ccgcgagccc tggttcgagg
     5161 gcggcgtctg gcgctatgcc gcggtgcagc tcggcggcat gtacgcgatc gccggccacg
     5221 ccgccgacca gctccgggcc cgccggcagg cgaccgcccc tctccaggca gcccggctca
     5281 ggcgcatggt gacagcgtgc gagaccgcgc gtctctgggt cgcctccgcg gccctggcga
     5341 cggaagcgcc ggcggctcaa ccggccaccg cccgggtggc ggtgctcgcc cggctcgagg
     5401 tcgccgaggc ggccgtccgc ctgctccagc tcgccgacga ggcgatgggt gcggcctcct
     5461 tcgcgctc
//
LOCUS       DAOTFI010000287         5465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_17716_length_5465_cov_6.375786, whole genome shotgun sequence.
ACCESSION   DAOTFI010000287 DAOTFI010000000
VERSION     DAOTFI010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17716_length_5465_cov_6.375786"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="P5558_21210"
     CDS             <1..633
                     /locus_tag="P5558_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26902.1"
                     /translation="IVHLPLGGLELAVDVSAASPVERIDVFNGRSLAATLRPHDEKDL
                     GRRIRVIWEGATYRGRFRQVVWDGSAKVTGDRIQDVVPIAFLNCDKTLDRVGEDGLAW
                     RSVTTGNLAGFEMQLEDGRSGRLTIETPLLKVDIPLADIGLEDHVTEAEGELPRLIRI
                     CRAPDTRSALTFSGTVRPELKAAGDNPLFVRVTLADGTRAWSSPIYVYRA"
     gene            complement(656..1324)
                     /locus_tag="P5558_21215"
     CDS             complement(656..1324)
                     /locus_tag="P5558_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY26903.1"
                     /translation="MSESRFVELRGQRIEITDARRRAILEEMPEIAWIANEELRAGVV
                     DAWAAAVASSSLERIGDMKPSGNFDSRPLRHGTQADHIRSVTRLAVKIAEEMDGLLPD
                     FAYDRDLLIAGSLCHDIGKVWEFDPENVRRWKAKPRKTGMPSLRHPGYGIHICLTLGL
                     PEEVAHMAAAHSGEGELLTRSLENTILHWADYTFWRVVEAGDQFATEDPWLDGMMSEL
                     SGKA"
     gene            complement(1342..2631)
                     /locus_tag="P5558_21220"
     CDS             complement(1342..2631)
                     /locus_tag="P5558_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRY26904.1"
                     /translation="MVPVALLLLIVLLALSLPVAAALAFLSLGLDQLYSPMALSRAMG
                     EIVWSASSNFLLVAVPLFVLLGEILLRSGIAERLYGATSQWLTWLPGGLMHANIGASM
                     AFAATSGSSVATAATIGTVATPLIDEYRYGERLFLGSLAAGGTLGILIPPSINLIIYG
                     WLTETSVPQLYLAGFIPGIVLGGLFMATIVICCLLRPAWAGRAPATSWQTRVSTLRDL
                     LPPLGIFLIVIGSIYAGFATPTESAALGVVAALMLAAAYRRLSLAMLIEALDGTMRTT
                     GMIMLIVAAAWFLNFILSAIGLVNALNDFIGNLGLGPTAMLAVVVVFYIVLGCFMEPL
                     PMMIVTVPVITPVVVSAGYDPVWFGILIVMLCETAMITPPVGVNLFVVQGVRRRGSIQ
                     DVMLGVLPFIASLLVMILLVIAWPDIVLWLPRLAGGF"
     gene            complement(2636..3226)
                     /locus_tag="P5558_21225"
     CDS             complement(2636..3226)
                     /locus_tag="P5558_21225"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY26905.1"
                     /translation="MPGIAGLIAGISLAMSRVGGLFLLVAAMLISIEILIRKFLFLPF
                     NIGTELSTYALAVGASWSFAYALLQRAHVRIDVLRGRLPASARTFLDLLALVSLVVFG
                     LILSWHVWGTVATSWKLGARENTPLATPLVIPQFMWLLGIAWFTLVAGIETARAIGAL
                     VRGDRAMVHRIAAPLGVEDELGTVLAPAAARPGSAG"
     gene            complement(3235..4302)
                     /locus_tag="P5558_21230"
     CDS             complement(3235..4302)
                     /locus_tag="P5558_21230"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY26906.1"
                     /translation="MRIIRLAGCAVLAALTAPLPAGAQDTTLQSVNLKVSGGNRTQNL
                     FRYVYGPFFTEELSERSNGAITTTFGSIEEMGIKGPEVLRLLSLGIFDISEGTISYMA
                     GEDPHFDGLDLPGLTLDIESQRQAVEAYKPVLAKIMSEKFNTRLLSMSPVALQVFYCR
                     DPIDGLASLEGKKIRVFNRAMADLVEAVGASTINLPFAEVVPAMQRGVADCAVTGTSA
                     GNTARWWEVTDYLYPLPMGWSMTFFAANNESWARLDPATQDFLLEEFAGLEDRMWAQA
                     GADVQDGINCNIGEGTCKDGIPAEPPMTLVELSADDRTQAQAILKDHVLPAWAERCGS
                     DCAKQWNATVGQVVGVEAPTD"
     gene            4471..5367
                     /locus_tag="P5558_21235"
     CDS             4471..5367
                     /locus_tag="P5558_21235"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY26907.1"
                     /translation="MTLAQLETFFWVARLGSTQATGRQLNLAQPTISLRLRDLETALG
                     LRLFDRVGRGLKLTHEGGELLERASAILNEVTRIGELSGGADGLRAVVRVGVPETLAL
                     VCLPSLLRTLQAEHPALRLELVVATSTDLEAQLLRHELDLAFLVDPTSDPRLRLTLLG
                     MQETGWFAAPGFGLSSPVRPQDLHHLPIVSNPHPSTMHEHIGEWFRSAGLELPRLSFC
                     NSLSVIAHLVRQGVFVGFLPRKMVEADLEAGAIRHLASRPPVPGARIYGGHRLAESSP
                     AILAILRATRQVVEQIDFLRPV"
BASE COUNT          911 a         1854 c         1913 g          787 t
ORIGIN      
        1 atcgtccacc tgcccctggg cggcctcgag ctcgccgtgg acgtctccgc cgcctcgccc
       61 gtcgagcgca tcgacgtctt caacggccgc agcctcgccg ccacgctccg cccgcatgac
      121 gagaaggatc tcggccggcg catccgcgtc atctgggaag gtgccaccta tcgcggccgc
      181 tttcgccagg tcgtctggga cggcagcgcc aaggtcaccg gcgaccgcat ccaggacgtc
      241 gtgcccatcg ccttcctcaa ctgcgacaag acgctcgacc gggtcggcga ggacggactc
      301 gcctggcgtt cggtgacgac cggcaacctc gccgggttcg agatgcagct cgaggacggc
      361 cggagcggtc gcctgaccat cgagaccccg ctcctgaagg tcgacatccc gcttgctgat
      421 atcggcctcg aggatcacgt caccgaggcc gaaggcgagc tgccccgcct gatccgcatc
      481 tgccgggcac cggacacccg atcggcgctg accttcagcg gcaccgtccg gcccgagctg
      541 aaggcggccg gcgacaaccc cctcttcgtc cgcgtcaccc tcgccgacgg cacccgcgcc
      601 tggtcgagcc cgatctacgt ctaccgcgcc tgatgaggca ggcgggcgga gccgctcagg
      661 ccttgcccga cagctcgctc atcatgccgt cgagccaggg gtcctcggtc gcgaactggt
      721 cgcccgcctc gaccacgcgc cagaacgtgt agtcggccca gtgcaggatg gtgttctcga
      781 gcgagcgggt gagcagctcg ccctcgccgg agtgcgcggc ggccatgtgg gcgacctcct
      841 cgggcaggcc gagcgtcagg cagatgtgga tgccgtagcc ggggtggcgg agcgacggca
      901 tgccggtctt gcgcggcttg gccttccagc ggcggacgtt ctcgggatcg aactcccaga
      961 ccttgccgat gtcgtggcag agggagccag cgatcaggag atcgcggtca taggcgaagt
     1021 cggggagcag cccgtccatc tcctcggcga tcttgaccgc gagccgggtc accgagcgga
     1081 tgtgatccgc ctgggtcccg tgcctgagcg ggcgcgaatc gaaattgccg gacggcttca
     1141 tgtcgccgat ccgctcgagg ctgctggacg caacggcggc ggcccaggcg tcgaccacgc
     1201 cggcgcgcag ctcctcgttg gcgatccagg cgatctcggg catctcctcg aggatcgccc
     1261 ggcggcgcgc gtcggtgatc tcgatgcgct ggccgcgcag ctcgacgaac cggctctcac
     1321 tcatgattga acactccagg gctagaagcc gcccgccagc cggggcagcc agagaacgat
     1381 atcgggccag gcgatcacca gcaggatcat gacgagcagg ctggcgatga agggcagcac
     1441 gcccagcatg acgtcctgga tcgagccgcg tcgacgcaca ccctggacga cgaagagatt
     1501 gaccccgacc ggcggcgtga tcatcgcggt ctcgcagagc atgacgatca ggatgccgaa
     1561 ccagacggga tcgtagccgg cgctcaccac gaccggcgtg atcaccggca ccgtgacgat
     1621 catcatcggc aggggctcca tgaagcagcc gagcacgatg tagaagacga cgacgacggc
     1681 gagcatggcg gtggggccga ggccgagatt gccgatgaag tcgttgagcg cgttgacgag
     1741 gccgatggcc gagagaatga agttgaggaa ccaggcggcg gcgacgatca gcatgatcat
     1801 gccggtggtg cgcatggtgc cgtcgagggc ctcgatcagc atcgcgagcg agaggcgccg
     1861 gtaggcggcg gccagcatga gcgccgccac cacgccgagg gccgccgatt cggtcggggt
     1921 ggcgaagccg gcatagatgc tgccgatgac gatcaggaag atgccgaggg gcgggagcag
     1981 gtcccggagg gtcgagacgc gggtctgcca gctcgtcgcc ggcgcgcgcc ctgcccaggc
     2041 cgggcgcaag aggcagcaga tgacgatcgt cgccatgaag aggccgccga gcacgatgcc
     2101 cgggatgaag cccgcgaggt agagctgcgg caccgaggtc tcggtcagcc agccatagat
     2161 gatcaggttg atcgagggcg ggatcaggat gccgagcgtg ccgcccgccg ccagcgagcc
     2221 gagaaagagc cgctcgccgt agcggtattc gtcgatcagc ggcgtggcca ccgtgccgat
     2281 ggtggcggcg gtcgcgacgc tcgagcccga ggtcgcggcg aaggccatgg aggcgccgat
     2341 attggcgtgc atcagcccgc ccggcagcca ggtgagccat tggctggtgg cgccgtagag
     2401 gcgctcggcg atgcccgagc gcaggaggat ctcgccgagc aggacgaaga gcggcacggc
     2461 gacgaggagg aagttgctgg aggccgacca gacgatctcg cccatcgccc gtgacagcgc
     2521 catgggcgag tagagctggt cgagccccag cgacaggaag gcgagagcgg cggcgaccgg
     2581 caggctgagc gccagcagca cgatcagcag gaggagggcg acagggacca cgatgtcagc
     2641 cggcgctgcc cggccgtgcc gccgccggcg ccagcaccgt gccgagctcg tcctcgacgc
     2701 cgagcggtgc ggcgatccgg tgcaccatcg ccctgtcgcc ccgcaccagg gcgccgatgg
     2761 cccgcgccgt ctcgatgccg gcgaccaggg tgaaccaggc gatgccgagg agccacatga
     2821 attgcgggat gaccagcggt gtcgccaaag gcgtgttctc ccgggcgccg agcttccacg
     2881 aggtcgcgac cgtgccccag acgtgccagc tcaggatcag cccgaaaacc acgagggaga
     2941 ccagcgccaa gaggtcgagg aaggtgcggg ccgatgccgg caaccggccg cgcagcacgt
     3001 ctatccgcac atgcgcccgc tgcaggaggg cgtaggcgaa cgaccagctc gcccccaccg
     3061 ccagcgcata ggtcgagagc tcggtgccga tgttgaacgg caggaacagg aacttgcgga
     3121 tcaggatctc gatcgagata agcatggcgg cgaccagcag aaagaggccg ccgacccggc
     3181 tcatcgccag cgagataccg gcgatgaggc ccgcgatgcc cggcacgccc cggctcagtc
     3241 ggtcggcgcc tcgacgccga cgacctggcc caccgtcgcg ttccactgct tcgcgcagtc
     3301 gctgccgcaa cgctcggccc aggccggcag gacgtggtcc ttgaggatcg cctgcgcctg
     3361 cgtgcggtcg tcggcggaga gctcgaccag ggtcatcggc ggctcggccg gaatgccgtc
     3421 cttgcaggtg ccctcgccga tattgcagtt gatgccgtcc tgcacgtcgg cgccggcctg
     3481 cgcccacatc cggtcctcga gcccggcgaa ctcctcgagc aggaagtcct gggtcgccgg
     3541 gtcgagccgc gcccagctct cgttgttggc cgcgaagaag gtcatcgacc agcccatggg
     3601 caggggatag aggtagtcgg tcacctccca ccagcgggcg gtattgccgg ccgaggtgcc
     3661 ggtcaccgcg caatcggcga ccccgcgctg catcgccggc acgacctcgg cgaagggcag
     3721 gttgatggtc gaggcgccca cggcctcgac gagatcggcc atggcgcggt taaagacgcg
     3781 gatcttcttg ccctcgagcg acgccagccc gtcgatggga tcccggcagt agaacacctg
     3841 cagcgccacc gggctcatgc tcaagagccg ggtgttgaac ttttccgaca tgatcttggc
     3901 caggaccggc ttgtaggcct cgaccgcctg gcgctggctc tcgatgtcga gcgtgaggcc
     3961 gggcaggtcg agcccgtcga aatgcgggtc ctcgccggcc atgtagctga tcgtgccctc
     4021 ggagatgtcg aagatgccga ggctcagcag gcggaggact tccggcccct tgatgcccat
     4081 ctcctcgatc gagccgaagg tcgtggtgat cgccccgttc gagcgttcgc tgagctcctc
     4141 ggtgaagaag ggcccgtaga catagcgaaa gaggttctgc gtccggttgc cacccgagac
     4201 cttgagattg acgctctgca gggtcgtgtc ctgcgcgccg gccggcagcg gtgcggtgag
     4261 agcggcgagc acggcacatc ccgcgagcct gatgatccgc atcggtaggt ccctcccgga
     4321 gctggcgcgg gcgcagtgcc cagcgaaatc tgatggcttg ctgagggccg agcgttccgc
     4381 ctgaggcacg agacaacgct agagccgatg gaacgacaaa tccaattgga ttatctcatg
     4441 ctcgcctatt aagtttcctg atagatgcgc gtgacactcg cccaactgga gacgttcttc
     4501 tgggtcgccc ggctcggctc gacccaggcc accgggcgcc agctcaacct ggcgcagccg
     4561 accatctcgc tgcggcttcg cgacctcgag accgccctcg gcctccggct gttcgaccgg
     4621 gtcggccgcg gcctcaagct gacccacgaa ggtggcgagc tgctcgagcg ggccagcgcc
     4681 atcctgaacg aggtgacgcg gatcggcgag ctgagcggcg gcgccgatgg cctgcgcgcc
     4741 gtggtgcggg tcggggtgcc cgagacactg gccctcgtct gcctgccgtc gctcctgcgg
     4801 acgctgcagg cggagcatcc ggccctgcgg ctggagctgg tcgtcgccac cagcaccgat
     4861 ctcgaggccc agctgctgcg gcacgagctc gatctcgcct ttctggtcga cccgaccagc
     4921 gacccgcggc tccggctgac gcttctcggg atgcaggaaa ccggctggtt cgccgccccg
     4981 ggcttcggcc tgagctcgcc ggtccggccg caggacctgc atcatctgcc gatcgtcagc
     5041 aacccgcacc cgtcgaccat gcacgagcat atcggcgagt ggtttcgctc ggccggtctc
     5101 gagctgccgc gcctgagctt ctgcaacagc ctctcggtga tcgcccatct ggtgcgccag
     5161 ggcgtgttcg tcggcttcct gccacgcaag atggtcgagg ccgacctcga agccggcgcc
     5221 atccggcacc tggcgagccg gccgccggtg ccgggcgcgc gcatctatgg cggccaccgt
     5281 ttggccgaga gcagcccggc cattctcgcc atcctcaggg ccacccgtca ggtcgtcgag
     5341 cagatcgatt tcctccggcc cgtttgaccg ccccctgcca gaagcaaaaa aagccgccgg
     5401 cgcgctctgg tgagcggccg gcgcagtcgg ggaggcacgg ggagaaaggg gaggctggcg
     5461 tcagc
//
LOCUS       DAOTFI010000288         5405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_17969_length_5405_cov_7.061308, whole genome shotgun sequence.
ACCESSION   DAOTFI010000288 DAOTFI010000000
VERSION     DAOTFI010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17969_length_5405_cov_7.061308"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="P5558_21240"
     CDS             <1..649
                     /locus_tag="P5558_21240"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRY26908.1"
                     /translation="AAPALATPGRVLMATVDVGAGLREALALAGDAAIARLGVPGGFA
                     DDPIVRIRLPGGLDRVREGLVEAGLGPLVLDLEARMNSAAELVMPATRPLLEAATASL
                     AIENAEDILAGADDSATRLLEGEAGSGLAAEINPLVAEAVVATGANAAFDAFVAEYSR
                     LPLMPNVAGKLTSHVVESSLAGLFLYMGEQERAIRRDSSARTTELLQAVFGASRG"
     gene            complement(678..2648)
                     /locus_tag="P5558_21245"
     CDS             complement(678..2648)
                     /locus_tag="P5558_21245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRY26909.1"
                     /translation="MLDFRRLRSCLVPLTAFHAFLIVWSAAPAAAQEAPQPRHVGSAS
                     CITCHESAAEAWQGSDHALAWTAPDEMTVLGDFDDATFDRQGVVTRFTRQGDAFAVET
                     EDGNGQRRSFDVVGVAGIHPLQQYLLSPEPGRTQAFDLAWDTVRHAWFDLYPGEKLRP
                     GDGMQWTGPYKSWEARCAECHATGFSRNYDATTRTYDPLVAEIGVACEACHGPGEAHN
                     AWAAAPDTFDPAAWPGLTPHGLTIDLAASAEVEIQQCAGCHSRREALFDGNPLPGTPY
                     HDAYTLELLRAGTYHADGQIDAEVYVYGSFLQSRMHAQGVRCSDCHEPHSTRLRAEGN
                     DVCTQCHSPAGNDRFPTLRKALYDDPSHHFHEADSDGAQCSACHMPQKVYMGVDGRRD
                     HGFRVPRPDLHALNDAPDTCTSCHADRDAVWAAAEIAGRFPDSIHRGPHFSTTFAAAR
                     RDPVAQSAALIALADNTDLAGIVRATALDLLEPVTDPTIADRTAPLLADPDPLVRGAA
                     AGLQRGLAPEARLDRLAPVLADPTRAVRIAAARAMLGADASRASDAVRASLARATDEW
                     HRSLSTRLDFPETNLQIAGTALVSRNFPLAEAAFREAVTLDPQLVDAWIMIARIRDAF
                     GDTAGARDALDDALANNPGQPDLVALRASLGP"
     gene            complement(2774..3505)
                     /locus_tag="P5558_21250"
     CDS             complement(2774..3505)
                     /locus_tag="P5558_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26910.1"
                     /translation="MPHESVAQYDSRLRGHSLLAFARQALKPGTRGQLARRLRTRLWA
                     DISAIGFRRDLHVAFTAPDARIPLEIRQLTAADAPAILGDDDPTLDVLERWERATRLR
                     LWTNGLGRCFAAIAPDGRPCFVQWIFLPVDNAGLRAYSGDTFPELAPGEALVEGAFTP
                     AAFRGKGVMPAAMARIVLEAGKLGARYVHTFVEVDNMPSLKGCIRAGFPAYQWREQHW
                     RLMRRRHEFGPLPADFATRFAAIYR"
     gene            complement(3653..4279)
                     /locus_tag="P5558_21255"
     CDS             complement(3653..4279)
                     /locus_tag="P5558_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HRY26911.1"
                     /translation="MASLAEPRYAVLYADPPWTFATYSRKGKGRSAEAWYDCMSLADI
                     KRLPVKDQAADDAILLMWATDPLLHRAFEVIEAWGFTYKTVGFYWVKLNKTAGTLVFD
                     RKSFFTGMGFWTRANPELCLLATRGKPKRRSAAVPKLIVSPRREHSRKPDEAYAAIER
                     LADGPYLELFARQTRPGWDAWGNQLGILDESVGEGPKRRWAATAYPDE"
     gene            4456..5382
                     /locus_tag="P5558_21260"
     CDS             4456..5382
                     /locus_tag="P5558_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY26912.1"
                     /translation="MTVLNRSGISTFAMATALAAAGWLAAGTDAQAQQKVRWQVPMAF
                     ASTLTALGDTMPYVADQLRRASGGAIDLQVFEPGTLIPALSVFENVSAGNMDAGYSWM
                     GYEWGQVPATALFGATPFGLEPVEFSAWMYFHGGQELLDEILQPYNVRALFCGSISPE
                     AAGWFREPLENVEQLQGLKMRAAGVGGEILSELGMSVTMLPGGELYQALETGVLDATE
                     FSLPTVDEQLGFYQVAKFYHLPGWHQPSTNQFLYINLDAWNGLDDQTQGMIEMGCQAG
                     VAYAMSRSEALQGAVLERFAAEGVELVGRQGI"
BASE COUNT          780 a         1785 c         2026 g          814 t
ORIGIN      
        1 cgctgcaccg gccctggcca ccccgggccg ggtcctgatg gccacggtcg atgtcggcgc
       61 aggcctgcgc gaggccctgg ccctggcggg cgacgcggcg atcgcgcgcc tcggcgtgcc
      121 cggcgggttc gccgacgatc cgatcgtgcg cattcgcctg cccggcgggc tggatcgggt
      181 gcgcgaaggc ctggtcgagg ccgggctcgg gccgctcgtc ctggacctcg aggcgcgcat
      241 gaacagtgcc gccgaattgg tgatgccggc aaccaggccg ctgctggagg cggccaccgc
      301 aagcctcgcc atcgagaatg cggaggacat cctggccggt gccgacgact cggcgacccg
      361 cctcctcgag ggcgaggcgg ggagcgggct ggccgccgag atcaatcccc tggtcgccga
      421 agccgtggtc gccaccggtg cgaatgccgc tttcgacgcc ttcgtcgccg agtattcgcg
      481 cctgccgctg atgcccaatg tcgccgggaa gctgaccagt cacgtggtcg agtcgagcct
      541 cgccgggctc ttcctctaca tgggcgagca ggagcgcgct atccgccgcg attcgtcggc
      601 ccgcacgacc gagctcctgc aggccgtctt cggcgccagc cgcggctgat cacatcgacc
      661 gctcggacga aaacgcccta tggcccgagc gaggcgcgca gggccacgag gtccggttgg
      721 ccggggttgt tggcgagcgc atcgtcgaga gcgtcccggg cgccggccgt gtcgccgaat
      781 gcgtcgcgga tgcgggcgat catgatccag gcgtcgacca gttgcgggtc gagggtgacc
      841 gcttcccgaa acgccgcttc ggcgagcggg aagttgcggc tgacgagggc ggtgccggcg
      901 atctgcaggt tggtctccgg gaagtcgagc cgggtcgaca gcgacctgtg ccactcgtcg
      961 gtcgcccggg cgagcgaggc tcgcacggcg tccgaggcgc gggaggcatc ggcgccgagc
     1021 atcgctctgg ccgcagcgat gcgcaccgcc cgcgtcgggt cggcgaggac gggcgccagg
     1081 cggtcgagcc gtgcctcggg ggcgaggccg cgctgcagtc ccgccgcggc gccgcggacc
     1141 agcgggtcgg gatcggcgag gagtggcgct gtccggtcgg cgatggtcgg gtcggtcacc
     1201 ggctcgagga ggtcgagcgc cgtggcacgg acgatgccgg cgaggtcggt gttgtcggcg
     1261 agggcgatca gcgccgccga ctgggccacg ggatcgcggc gggcggcggc gaaagtggtg
     1321 gagaaatggg ggccgcggtg gatgctgtcc gggaagcgcc cggcgatctc tgccgcggcc
     1381 catacggcat cgcggtcggc gtggcaggag gtgcaggtgt ccggcgcatc gttcagggcg
     1441 tggagatccg ggcgtggcac ccgaaagccg tgatcgcggc ggccgtcgac gcccatgtag
     1501 accttctgcg gcatgtggca ggcgctgcac tgggcaccat ctgaatccgc ctcgtggaaa
     1561 tggtggctcg ggtcgtcgta gagcgccttg cggagggtcg ggaagcggtc gttgccggcg
     1621 ggtgagtggc actgggtgca gacgtcgttg ccctcggccc ggagcctcgt gctgtgcggc
     1681 tcgtggcagt cgctgcaacg cacaccctgc gcatgcatcc tggactgaag gaacgagccg
     1741 tagacgtaga cctcggcatc gatctggccg tccgcgtggt aggttccagc ccgcaggagc
     1801 tccagggtat aggcgtcgtg gtaaggggtg ccgggcaggg ggttgccgtc gaacagcgcc
     1861 tcgcgccgcg agtggcagcc ggcgcattgc tgaatctcga cttcggccga ggcggcgaga
     1921 tcgatcgtca agccatgcgg tgtcaggccc ggccaggcag ccgggtcgaa ggtgtccggc
     1981 gccgcggccc aggcgttgtg ggcctccccg gggccgtggc aggcctcgca ggccacgccg
     2041 atctcggcca cgagcgggtc gtaggttcgg gtggtggcgt cgtagttgcg actgaagccg
     2101 gtggcgtggc actcggcgca gcgcgcctcc cagctcttgt aggggccggt ccactgcatc
     2161 ccgtcacctg gacgcagctt ctcgcccgga tagaggtcga accacgcgtg gcgcaccgtg
     2221 tcccaggcga ggtcgaaggc ctgggtccgg ccgggctccg gggagaggag gtattgctgc
     2281 agcgggtgga tgccggccac accgaccacg tcgaagctgc gccgctggcc gttgccatcc
     2341 tcggtctcga cggcgaaggc gtcgccctgg cgggtgaagc gcgtcaccac accttgccga
     2401 tcgaaggtgg cgtcgtcgaa gtcgccgagg acggtcattt cgtcgggcgc ggtccaggcg
     2461 agcgcatggt cggagccttg ccaggcttcg gccgcgcttt cgtggcaggt gatgcagctc
     2521 gccgatccga catgacgcgg ctgcggcgcc tcctgggcgg cagccggcgc ggcgctccag
     2581 acgatcagga aagcatggaa agccgtcagg ggcacgagac aggaacggag gcggcggaag
     2641 tcgagcatgg cgatccttcg ccggcaacgc gccggcatcg ccgagcctag gagcccgatt
     2701 gggtggtggc cagccccaag cgcgtggcga gccgctcgcg acggcgccct cgcagcctcc
     2761 cggcgtggcg ggtctagcga tagatcgccg cgaaccgcgt ggcgaaatcc gccggcaacg
     2821 ggccgaactc gtgccggcga cgcatcaggc gccagtgctg ctcgcgccat tgataggccg
     2881 gaaagccggc gcggatgcag cccttgagcg acggcatgtt gtccacctcg acgaaggtgt
     2941 ggacgtagcg ggcgccgagc ttccccgcct ccaggacgat ccgcgccatg gccgccggca
     3001 tgaccccctt gccgcggaag gcggccgggg tgaaggcgcc ctcgacaagc gcctcgccgg
     3061 gcgccagctc ggggaaggtg tcgccggaat aggcgcgcag gccggcattg tcgacgggga
     3121 ggaagatcca ctgcacgaag cacggccggc cgtcgggcgc gatggcggcg aagcaccggc
     3181 cgagaccgtt cgtccagagc cgcagccggg tcgcccgctc ccagcgctcg aggacatcca
     3241 gcgtcgggtc gtcgtcgccg aggatggccg gggcgtcggc ggcggtgagc tggcggatct
     3301 ccagcggaat cctggcgtcg ggagcggtga aggccacgtg caggtcgcgc ctgaagccga
     3361 tcgccgagat gtcggcccag agccgggtcc ggagccgccg cgcgagctgg ccgcgcgtgc
     3421 ccggcttgag cgcctggcgg gcgaaggcca gcagcgaatg gcctcggagt cggctatcgt
     3481 actgggcaac ggactcgtgt ggcacgtgcg cgctccgctg gcagtcggct cgatcgcttc
     3541 gcgagtggga gcttgcgcca ttcgttccgc cgccaccacg ccgtcggccg tcatcagcca
     3601 ccttgctatg cccacccgtt aaccatgcgc tcggccaccc tcgccggcgc cgtcactcgt
     3661 cgggataggc ggtggccgcc cagcggcgct tgggaccctc gccgacagac tcgtcgagga
     3721 tgccgagctg gttgccccag gcatcccagc cggggcgggt ctggcgggcg aagagctcga
     3781 gataggggcc gtcggccagc cgctcgatgg ctgcgtaggc ctcgtcgggc ttgcggctgt
     3841 gctcgcgccg cgggctgacg atcagcttcg ggacggcggc cgagcgccgc ttcggcttgc
     3901 cgcgggtggc gagcaggcag agctcgggat tggcccgcgt ccagaagccc atgccggtga
     3961 agaagctctt gcggtcgaag accagcgtgc ccgccgtctt gttgagcttc acccagtaga
     4021 agccgaccgt cttgtaggtg aagccccagg cctcgatcac ctcgaacgcc cggtgcagca
     4081 ggggatcggt cgcccacatc aggagaatgg cgtcgtccgc cgcctggtcc ttgaccggca
     4141 ggcgcttgat gtcggcgagg ctcatgcagt cgtaccaggc ctcggcgctg cgccccttgc
     4201 ccttgcgcga gtaggtcgcg aaggtccagg gcgggtcggc gtagagcacg gcgtagcgtg
     4261 gttcggcgag gctggccatg cgcttcgata gagcaaggcg ggtgcctgat aaagccgcga
     4321 tcgccgctcg gtcgaacgaa ggggtggcgg tgcacagccg agaccgtcta taaatgcatg
     4381 ggcatcatgc accgatagca gtgcccggga tgaccaggga ggcgcggcag ccggcatcgg
     4441 cgtcgccaga gaaggatgac cgtcttgaac agatccggca tttcgacctt cgccatggcc
     4501 acggcattgg ctgccgctgg ctggctcgcg gccggcaccg acgcccaggc gcagcagaaa
     4561 gtgcgctggc aggtgccgat ggccttcgcc tcgaccctca cggcgctcgg cgacaccatg
     4621 ccctacgtcg ccgaccagct ccggcgagcg agcggtggcg ccatcgacct gcaggtcttc
     4681 gagccgggca cgctcatccc ggcgctctcc gtcttcgaga acgtctcggc cggcaacatg
     4741 gacgccggct attcgtggat gggctacgaa tgggggcagg tgccggccac cgcgctcttc
     4801 ggcgccacgc cgttcggcct cgagccggtc gagttctccg cctggatgta cttccacggc
     4861 ggccaggagc tgctcgacga gatcctgcag ccctacaacg tgcgagcgct cttctgcggc
     4921 tcgatcagtc cggaggccgc cggctggttc cgcgagccgc tcgagaacgt cgagcagctc
     4981 caggggctga agatgcgcgc cgccggcgtc ggcggcgaga ttctctccga gctcggcatg
     5041 tcggtcacca tgctgcccgg cggcgagctc taccaggcgc tcgagaccgg cgttctggac
     5101 gccaccgagt tctcgctgcc gaccgtggac gagcagctcg gattctatca ggtcgcgaag
     5161 ttctaccatc tgccgggctg gcatcagccg tcgaccaacc agttcctcta catcaatctc
     5221 gatgcgtgga acggtctgga cgatcagacg cagggcatga tcgagatggg ctgccaggcc
     5281 ggcgtggcct atgccatgtc tcgctccgag gcgctgcaag gtgcggtcct cgagcgcttc
     5341 gccgcggagg gcgtcgagct ggtcgggcgg cagggcatct agcggaaaac aatcgaccca
     5401 gcttt
//
LOCUS       DAOTFI010000289         5393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_18029_length_5393_cov_7.773885, whole genome shotgun sequence.
ACCESSION   DAOTFI010000289 DAOTFI010000000
VERSION     DAOTFI010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18029_length_5393_cov_7.773885"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="P5558_21265"
     CDS             complement(<1..919)
                     /locus_tag="P5558_21265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY26913.1"
                     /translation="MRWTSSVRNFFLMPAVIWVLVFTIFPLGYSLYAAFHNVTNETIV
                     NRMEVPRLDKAGEPVLRPDGTPRMKTEVVRETVTKWEFVGLDNFKRLFTDTVLHEAIK
                     VTAIFVGVGLAIQMTLGLALAMLFNRDMPGRSLLRTIMILPIFTTPVAAGYLFFTIFY
                     EEGGPLGWTGIPWLSNPSWALFSVIVVDVWQWTPFCFLVFLAALQGIPDELLEAVKLD
                     TNSNWTTFREVLLPLLMPTIVIVLLLRFAEAMKVFDIVASLTVGGPGNATQSLSYLAF
                     RTGLRFFDVGYASAIAYTLLALVMVVVTIL"
     gene            complement(1010..2650)
                     /locus_tag="P5558_21270"
     CDS             complement(1010..2650)
                     /locus_tag="P5558_21270"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY26914.1"
                     /translation="MNRLNALLAGASALALLAGPASAGEYVEWQDDGSVLLKLSPEYD
                     SAVIEVPAGELGSLFGPDEQPFAGDEITVLTLDSGPKGGISGPIYAFRPVFEELTGAK
                     VNIALVPISELYTKIFLDLRNGTGEYDGMIVGAFMYGDLIDGNYILPVDDWQQSGDFP
                     AWSYDVMPDALRTIYTWDGVGYGVLSDADGQVLYYRRDLLNDPEHQAAFKAEHGYDMP
                     VPPATLQQLRDIAAYFDGKNFDANDPEPDSGVVLHLKVREQGLYHFMTLATSFAVTPG
                     ELSETQGVYWFNPTDMTPLINSPGHVKALEYLKDLAQFGPDAQVSWSLGEAWDYFLRG
                     KAVFNFSYGDVAPLAQDESRSNIRGKIGSTILPASDTYWDMNKQEFVTTDTPRKVGNV
                     TGGSWHGVVSSLSDSPETTYAFWSLMATKPISKWLATYGWDGVDPGYSYQFLEPVGEA
                     KLEDYLAAGWDATDVQEYLQAYYDNFNAEVMLPYLRITGTQEYYDILDSNLSAAMSGA
                     KTPQQALDDTAAGWEQVTDRLGREKQLEAYQAAIGWQG"
     gene            complement(2790..3476)
                     /locus_tag="P5558_21275"
     CDS             complement(2790..3476)
                     /locus_tag="P5558_21275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY26915.1"
                     /translation="MDTRSDSAEIERLRAELDVLLYATSHDLAEPARTVRGFLDLLQR
                     RYGSALDDDAREFIGFAVDAAGRLEVMLAGLLELGRIGRQEPAHVALDLAAIAEAAAA
                     GFRPNVTAAGGKLEIGELPACEGDPDLWRRLIAILIDNALTYRSAAPPVIRIDGEAGR
                     ITIADNGIGIAAEFHERIFEPFQRLHTRDAIPGVGMGLAIARKIAERQGRRIELSSSP
                     GTGSRFAIVG"
     gene            complement(3529..4107)
                     /locus_tag="P5558_21280"
     CDS             complement(3529..4107)
                     /locus_tag="P5558_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRY26916.1"
                     /translation="MRGPGTSSLSLSACPALVLNADYQPLSYFPLSLWNWQETVKAIF
                     LDRVAVVQHYDRSVRSPAFEMRLPSVVALKEYIPQSRRPPFTRFNVFLRDRFTCQYCQ
                     TGLPAPELTFDHVIPRSRGGVTTWSNIVTACNRCNLRKGSRLPKECSMHPMQKPLIPS
                     THQLQRNGRAFPPNYLHESWRDYLYWDTELEP"
     gene            complement(4281..>5393)
                     /locus_tag="P5558_21285"
     CDS             complement(4281..>5393)
                     /locus_tag="P5558_21285"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY26917.1"
                     /translation="LRAAGLVVFGKTAAPELGVNVTTEPVLHGPTRNPWDPTRSAGGS
                     SGGAAVAVAAGILPMAHGGDGGGSIRIPASACGLVGLKPSRARNPIGPVVGEAWNGLV
                     AGHVLSRSVRDTALALDATAGPEPGDPYACPPIAGSFAAAAGRDPQPLRIGFVTSLPG
                     GSPVEGACIAATEAAARKLQDLGHHLEELMLPLDAQRLRRTMETIVAVNLARDLDFWA
                     ETLGRPADRTAVESCTWLLAGRGRRVRATEYGRAITTLHQTSRQLGQVFGRHDLILTP
                     TLGTLPPPLGLLDQNMDDLDRFLELNATFIPFTPLYNMTGCPAISLPLEQSPDGMPIG
                     VMLGAALGREDLLLAVAGQLERAHPWFDRVPPERAA"
BASE COUNT          922 a         1714 c         1896 g          861 t
ORIGIN      
        1 agaggatggt cacgaccacc atgaccaggg cgagcagcgt gtaggcgatg gccgaggcat
       61 agccgacgtc gaagaagcgc agccccgttc gaaaggccag ataggagagc gactgcgtgg
      121 cgttgcccgg cccgccgacg gtcagcgatg ccacgatgtc gaagaccttc atggcctcgg
      181 cgaagcgcag cagcagcacg atgacgatgg tcggcatcag gagcggcagc aggacctccc
      241 tgaaggtcgt ccagttggag ttcgtgtcga gcttcacagc ctccagaagc tcgtccggga
      301 tgccctgcag ggctgccagg aagacgagga agcagaaggg cgtccactgc cagacgtcga
      361 cgacgatgac cgagaagagt gcccaggacg ggttggagag ccagggaatg ccggtccagc
      421 ccaaggggcc gccttcctcg tagaagatcg tgaagaagag atagccggcg gcgaccggtg
      481 tcgtgaagat cggcaggatc atgatcgtgc gcaagagaga tcgaccgggc atgtcgcgat
      541 tgaacagcat ggccagcgcc aggccgagcg tcatctggat ggccagcccg acaccgacga
      601 aaatcgccgt gaccttgatc gcctcgtgca gcaccgtatc ggtgaacagc cgcttgaaat
      661 tgtcgagccc gacgaactcc cacttggtga ccgtctcgcg tacgacctcg gtcttcatgc
      721 gcggcgtgcc gtccggacgg aggacgggct cgcctgcctt gtcgaggcgc ggcacctcca
      781 tgcgattgac gatggtctcg ttcgtgacgt tatggaacgc cgcgtagagc gagtagccga
      841 gcgggaagat ggtgaacacc agcacccaga tgacggccgg catcaggaag aaattgcgga
      901 cggagcttgt ccagcgcatc ggcggtcacc actccggatc gggctcgagc atggtggaaa
      961 ggaggcgccg cccggccgaa gccgggcggt cctcaggtcg acgggtggtt cagccctgcc
     1021 agccgatggc tgcctgatag gcctcgagct gcttctcgcg gccgagccgg tcggtgacct
     1081 gctcccagcc ggcggccgtg tcatccaacg cctgctgcgg cgtcttggcg ccgctcatcg
     1141 ccgccgacag gttcgagtcc aggatgtcgt agtattcctg ggtcccggtg atgcgcaggt
     1201 agggcagcat cacctcggcg ttgaagttat catagtacgc ttggaggtat tcctgcacgt
     1261 cggtcgcgtc ccagccggcg gccaggtagt cctcgagctt ggcctcgccg accggctcca
     1321 ggaactggta gctgtagccc gggtcgacgc cgtcccagcc ataggtcgcg agccacttcg
     1381 agatcggctt ggtcgccatc agcgaccaga aggcataggt cgtctccggc gaatccgaca
     1441 gcgaggacac cacgccgtgc cacgagccac cggtgacgtt gccgaccttg cgcggcgtgt
     1501 cggtggtgac gaactcctgc ttgttcatgt cccagtaggt gtccgaggcc ggcaggatgg
     1561 tcgagccgat cttgccgcgg atgttcgagc gcgattcgtc ctgggcgagc ggcgccacgt
     1621 cgccatagga gaagttgaag accgccttgc cgcgcaggaa atagtcccag gcctcgccga
     1681 ggctccagct gacctgcgca tccgggccga actgggccag atccttcagg tactcgagcg
     1741 ccttgacgtg gccggggctg ttgatcagcg gggtcatgtc ggtcgggttg aaccagtaga
     1801 caccctgggt ctcgctgagc tcgcccggcg tgacggcgaa ggacgtagcc agcgtcatga
     1861 agtggtagag accctgctcg cgcaccttga ggtgcagcac gacaccggaa tcgggctcgg
     1921 ggtcgttggc atcgaagttc ttgccgtcga aataggcagc gatgtcccga agctgctgca
     1981 gggtcgcggg cggcaccggc atgtcgtagc cgtgctcggc cttgaaggcc gcctggtgct
     2041 ccgggtcgtt gagcaggtcg cggcggtagt agaggacctg gccgtcggca tcgctgagga
     2101 cgccgtagcc gacgccgtcc caggtgtaga tcgtccgcag ggcatccggc atcacgtcgt
     2161 aggaccaggc cgggaaatca ccgctctgct gccagtcgtc gaccggcagg atgtagttgc
     2221 cgtcgatcag gtcgccatac atgaaggcgc cgacgatcat gccgtcatat tcgccggtgc
     2281 cattcctgag atcgaggaag atcttggtgt agagctcgct gatcggcacg agggcgatgt
     2341 tgaccttcgc cccggtcagc tcctcgaaga ccgggcgaaa ggcgtagatc gggccggaga
     2401 tcccgccctt ggggccggaa tcgagggtga ggacggtgat ctcgtcgccg gcgaagggct
     2461 gctcgtccgg cccgaacagg ctgccgagct caccagccgg aacctcgatc accgccgaat
     2521 cgtactctgg tgacagcttc agcagcacgc tgccgtcgtc ctgccattcg acatactcgc
     2581 cggccgaggc cggtccggcc agcagcgcga gtgcggatgc gccggcgagc agcgcgttga
     2641 gcctgttcat gaatcgagcc tcccaattgc ccgtgcggca ggtttcgtta caatcgctaa
     2701 gccgcagcgc aagtgttagc gcattttcca cgacgttcaa tcaggatgat gcgcgcggcc
     2761 catgccggat cgaaaccaat cccccgcggt cagccgacga tcgcgaagcg gctgccggtg
     2821 ccggggctgg agctcagctc gatccgccgg ccctgacgct cggcgatctt gcgcgcgatc
     2881 gccagcccca tgccgacacc gggaatggcg tctcgcgtgt gcagccgctg gaagggctcg
     2941 aagatccgct cgtggaactc ggcggcgatg ccgatgccgt tgtcggcgat ggtgatgcgg
     3001 ccggcttcgc catcgatgcg gatgactggc ggtgccgcac tccgataggt cagcgcattg
     3061 tcgatgagaa tggcgatgag gcgtcgccag aggtcggggt cgccctcgca ggccggcagc
     3121 tctccgatct ccagcttgcc gcccgcggct gtcacattcg gccgaaaacc cgctgcggca
     3181 gcctcggcga tcgccgccag atcgagggcg acgtgcgccg gctcctgccg gccgatgcga
     3241 ccgagctcga gcaggccagc gagcatcacc tcgagccggc ccgccgcgtc gacggcgaag
     3301 ccgatgaact ccctggcatc gtcgtcgaga gccgacccgt agcgccgctg cagcaggtcg
     3361 agaaagcccc ggacggtgcg cgcgggctcc gccagatcgt ggctcgtggc gtagaggagc
     3421 acgtccagct ccgcgcgcag ccgctcgatc tcggcactgt cggatcgcgt atccattgag
     3481 tgaggctagg aaaaattcgc ttcgggcgct agcccgaact gccgtggctc agggctcgag
     3541 ctcggtgtcc caatagagat agtcgcgcca gctctcgtgc aggtagttgg gcgggaaggc
     3601 ccggccgttg cgctgtagct ggtgggtcga cgggatcagc ggcttctgca tgggatgcat
     3661 gctgcattcc ttcggcagcc ggctgccctt gcgcagattg cagcgattgc aggcggtgac
     3721 gatgttgctc caggtcgtca cgccgccacg cgagcgcggg atcacgtggt cgaaggtcag
     3781 ctccggcgcc ggcagccccg tctggcagta ctggcaggtg aagcggtcac ggaggaagac
     3841 gttgaagcgg gtgaatggcg ggcgccggct ctgggggatg tattccttga gcgccaccac
     3901 gctgggcagc cgcatctcga aggcgggcga gcgcaccgac cggtcgtagt gctgcaccac
     3961 ggcgacacga tcgaggaaga tcgccttgac cgtttcctgc cagttccaga gcgaaagcgg
     4021 gaaatagctg aggggctggt agtcggcatt caacaccagg gccgggcagg cggacagcga
     4081 caacgatgac gtgcccggcc cgcgcatcag gcctgcctag tcttgggttg tcgatgttct
     4141 ccgagccaca tctcgacgct ccgcggtcgc gagaccacca gatcgactta gctgcgatag
     4201 tgctaaacca gccgcatgac gctttcaagg caagacgggg gcaaagccag aggcgggcat
     4261 gccggcgatc gccgccgcgc tcaggcagcg cgctcgggcg gcacgcgatc gaaccatgga
     4321 tgggcacgct cgagctggcc ggctacggcg agcagcagat cctcgcgacc gagggcggcg
     4381 ccgagcatga cgccgatcgg catcccgtcc ggtgactgct cgagcggcag gctgatcgcc
     4441 gggcaaccgg tcatgttgta gagcggcgtg aagggaatga aggtggcgtt cagctccagg
     4501 aaccggtcga gatcgtccat gttctgatcg agcaggccga gcggcggcgg cagggtgccg
     4561 agcgtcggcg tgaggatcag gtcgtggcgg ccgaagacct ggccgagctg gcgcgacgtc
     4621 tggtgcagcg tggtgatcgc ccgaccgtac tccgttgccc ggacccggcg accacgcccg
     4681 gcgaggagcc aggtgcagct ctcgaccgcc gtgcggtccg ccggcctgcc gagcgtctcc
     4741 gcccagaagt cgaggtcgcg agcgaggttg acggcgacga tcgtctccat cgtccgccgc
     4801 aggcgctgcg cgtcgagcgg cagcatcagc tcttcgagat gatggccgag atcttggagc
     4861 ttcctggccg cggcctcggt cgccgcgatg cacgctcctt caactggtga accgcccggc
     4921 agcgaggtca cgaagccgat gcgcagcggc tgcgggtccc gcccggcggc ggcagcgaag
     4981 ctgccggcaa tcggcgggca ggcataggga tcgcccggtt ccggcccggc cgtcgcatcc
     5041 agggccaggg ccgtatcgcg cacgctgcgg ctcaggacat ggcccgccac cagcccgttc
     5101 caggcttcgc cgaccacggg cccgatcggg ttgcgggcgc ggctgggctt caggccgacg
     5161 agaccgcagg cggacgccgg gatgcggatg gagccgccgc cgtcaccgcc atgggccatc
     5221 ggcaggattc ccgcggccac cgccaccgct gccccacccg agctgccgcc ggcgcttcgc
     5281 gtcgggtccc aggggttgcg ggtcggcccg tgcagcacgg gctcggtggt cacgttgacg
     5341 ccgagctcgg gtgcggcggt cttgccgaag acgacgagcc cggcggcgcg cag
//
LOCUS       DAOTFI010000290         5315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_18383_length_5315_cov_6.014639, whole genome shotgun sequence.
ACCESSION   DAOTFI010000290 DAOTFI010000000
VERSION     DAOTFI010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18383_length_5315_cov_6.014639"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="P5558_21290"
     CDS             <1..293
                     /locus_tag="P5558_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRY26918.1"
                     /translation="TGSRMDEVIFEEFKGTGNSEIVLDRRVADKRVFPALDINKSGTR
                     REELLVDKATLQRMWVLRRILNPMGTTDSVEFLMDKMRLAKTNDDFWGTMNS"
     gene            complement(315..1148)
                     /locus_tag="P5558_21295"
     CDS             complement(315..1148)
                     /locus_tag="P5558_21295"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HRY26919.1"
                     /translation="MSRPVFFAQVSDFHIRPPGVIFADVVDTLAMLRAAVDTLVGFSP
                     KLDAVLVSGDLTNDGEAEAYAQIAPELARLPMPVYVVPGNHDHRERITALAGVAEVGQ
                     GNAHRQFTAELGSLHLIALDSLVEGEGHGALCAERLAWLETELERAAGRRILIMIHHP
                     PAATGIDFMDRIGLRSSPALDALIRRHAGSIERIVCGHVHRPIFYRWQGVPVSVTPGV
                     AHQVVLGLDGREPGFNLEPPGFHLHRWTETEGLVTHHVHLGSYPGPYYFSGNPDRKPA
                     T"
     gene            complement(1145..2125)
                     /locus_tag="P5558_21300"
     CDS             complement(1145..2125)
                     /locus_tag="P5558_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRY26920.1"
                     /translation="MAMQIKVGRHGGPEVLERAEAEVGAPGPGQVRLRHTAIGLNFID
                     TYFRSGLYPPPAGLPFVPGLEAAGVVEAVGEGVDGLEVGQRVAYGTGPLGAYSTERLI
                     PAEKVVAIGDKIADETAAAMMLKGLTAQYLLRQTYVVKPGDTILFHAAAGGVGLIACQ
                     WAKHLGATVIGTAGSQKKADLARAHGCDHVILYRDEDVPGRVKELTGGKGVPVVYDGV
                     GKDTFVMSLDCLRPRGLLVSFGNASGAVEGVNLGILAAKGSLFVTRPTIAGYTSTRAE
                     LEAAARDVVDVVGSGAVKIEVNQRFPLAQAADAHRALEGRKTTGQTVLLP"
     gene            2280..2573
                     /locus_tag="P5558_21305"
     CDS             2280..2573
                     /locus_tag="P5558_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HRY26921.1"
                     /translation="MKFNIEIECTPEEARHFLGLPEVAPMQQRLIEEIEAQLKKTVHE
                     MDGKAMLEQWLPIGMKGVEQFQSMWTQLAAAAAGVQRNRKPSDEGGADKSRKP"
     gene            2587..3912
                     /gene="mnmE"
                     /locus_tag="P5558_21310"
     CDS             2587..3912
                     /gene="mnmE"
                     /locus_tag="P5558_21310"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRY26922.1"
                     /translation="MNERRATVFAPATPPGRSALGVLRISGPAAAPAAEQLAGRVPEP
                     RRATVAELRDPASGALLDRALLLFFPAPSSSTGEDVLEIQHHGGAAVARSLVEALGAM
                     PELRPAEPGEFSRRAFLAGKLDLTAAEGLADLIDATSAAQARAALRQMDGALGALYVD
                     WRERLLRALALLEAEIDFAAEEEVPDRLWAFVEPDLAALAADLRRHLDDGRRGERLRR
                     GIAIAVIGAPNVGKSSLVNAIARRDVAIVTPFAGTTRDVIEVALDLASLPVTLLDTAG
                     LRDSDDPIELQGIARAKARAASADLRLLVVDDPADLPEAEGNDPTTLRVLNKLDLCRR
                     HDLLPGVIGVSATTGEGLDGLLAAVAAVARDLLPGTDEVLLTRARHRTALHDAQNALE
                     RALALGVAGDLGLLAEEVRLAGRALGRITGAFGVEDILDRVFSEFCIGK"
     gene            complement(3949..>5315)
                     /locus_tag="P5558_21315"
     CDS             complement(3949..>5315)
                     /locus_tag="P5558_21315"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY26923.1"
                     /translation="LALAVTENPLVPDEVQHLVAVLATGYVLFTLFVQATTLRWLIRL
                     VGLDRLSPLEEILRQRAILLTHGEIVDTLRDAAAERMLDPASVEAVANDYRQQDAAAV
                     ARPVGEEMQRRQVVLALLTLSRREAELYAEERARRMIARSANAALVRQVGALIDALRA
                     DGLEGYRYLARDQVRFDRATRFAAFLHRRLRWDTPLARRLTLHLERDIVQRYVLSELM
                     TFNTERIRGLFGERVSEVAGLFLETRLDQVERAIDAMRLQYPDYWASFGKRYLALAAL
                     RLEYAAIEQMEQERLLSPQVAHMMGQALRQRRTRLERPPHLDLGLRTAELIRHMPLFA
                     KLDEKEIKQLARQLKTRLALPGERIVRRGEPGDAMFFIATGAVEVRVQPEPVRLGTGD
                     FFGELALLTRRPRNADVVAIGFCRLLILRRELFEQVMGSHSVLGRRVREVATRRHAER
                     TSAG"
BASE COUNT          810 a         1856 c         1881 g          768 t
ORIGIN      
        1 acaccggctc gcgcatggac gaggtgatct tcgaggagtt caagggcacc ggcaattccg
       61 agatcgtcct cgaccgccgg gtcgccgaca agcgcgtctt cccggccctc gacatcaaca
      121 agtcgggcac gcggcgcgag gagctcctgg tcgacaaggc gacgctgcag cgcatgtggg
      181 tgctgcgccg catcctcaat cccatgggga cgaccgattc ggtggagttc ctcatggaca
      241 agatgcgcct cgccaagacc aacgacgatt tctggggcac gatgaactcg tagctgcccc
      301 cgccggcagc cccttcaggt cgccggcttc ctgtccgggt tgccgctgaa gtagtagggc
      361 cccggatagc tgccgagatg gacgtggtga gtcacgaggc cctcggtctc ggtccagcgg
      421 tgcaggtgga agcccggcgg ctccaggttg aagccgggct cgcgcccgtc gaggccgagc
      481 accacctggt gcgccacacc cggcgtcacg ctcaccggga caccctgcca gcgatagaag
      541 atcggccggt gcacatggcc gcagacgatc cgctcgatcg agccggcgtg gcgccggatg
      601 agggcgtcca gcgccgggct cgaccggagc ccgatgcggt ccatgaaatc gatgccggtg
      661 gcggccggcg ggtgatggat catgatcagg atccgccgcc cggcggcccg ctcgagctcg
      721 gtctcgagcc aggccagccg ctcggcgcaa agcgccccgt gaccttcgcc ctcgaccagg
      781 ctgtcgaggg cgatcaggtg aagcgagccg agctcggccg tgaactgccg gtgcgcgttg
      841 ccctggccga cctccgcgac gccggcgagt gcggtgatcc gctcgcgatg atcgtgattg
      901 cccggcacca catagacggg catcggcagc cgggcgagct ccggggcgat ctgcgcatag
      961 gcttcggcct cgccgtcatt ggtgaggtcg cccgagacca gcaccgcgtc gagctttggg
     1021 ctgaagccga cgagcgtgtc gacggccgcc cgcagcatgg ccagcgtgtc gaccacgtcg
     1081 gcgaagatca cccctggcgg acggatgtgg aaatccgaga cctgggcgaa gaagaccggt
     1141 cggctcatgg caggagcacg gtctgtccgg tcgtcttgcg gccctcgagc gcccggtgcg
     1201 cgtcggcggc ctgggcgagc gggaagcgct ggttgacctc gatcttcacc gcccccgagc
     1261 cgacgacatc caccacatcc ctggcggcgg cctcgagctc ggcccgggtc gaggtgtagc
     1321 cggcgatggt cggccttgtg acgaagagcg agcccttggc ggcgaggatg ccgagattga
     1381 ctccctccac cgcgcccgag gcattgccga agctgaccag gaggccgcga ggccgaaggc
     1441 agtcgagcga catgacgaag gtgtccttgc cgacgccatc atagaccacg ggcacgccct
     1501 tgccgccggt cagctccttg acccggcccg gcacgtcctc gtcacggtag aggatgacgt
     1561 ggtcgcagcc atgcgccctg gccaggtcgg ccttcttctg gctgcctgcc gtgccgatca
     1621 cagtggcgcc cagatgcttc gcccactggc aggcgatcag cccgacgccg ccggccgccg
     1681 cgtggaagag gatcgtgtcg cccggcttca ccacataggt ctggcgcagc agatactggg
     1741 cggtcaggcc cttgagcatc atcgccgccg ccgtctcgtc ggcaatcttg tcgccgatgg
     1801 cgaccacctt ctcggccggg atcagccgct cggtgctgta ggcgccgagc gggcccgtgc
     1861 cataggccac gcgctggccg acctcgaggc cgtcgacgcc ctcgcccacc gcctcgacga
     1921 cgcccgcggc ctcgaggccc ggaacgaagg gcaggccggc cggtggcggg tagagcccgg
     1981 agcggaaata ggtgtcgatg aaattcaggc cgatcgccgt gtgcctgagc cggacctgcc
     2041 cgggcccggg cgctcccacc tcggcttccg cccgctcgag cacctcgggc ccgccgtgcc
     2101 gcccgacctt gatctgcatc gccatcgatc gctccctccg tctgctggct tcggagctag
     2161 gccggaacgc gctcgcgggc aaggccggcg gcgaccactc ccggctgttt ctggcgtcac
     2221 cccggtctcc tgacctatag tcgcggcgag tcggcgtcag cagcggatgg ggaacggcct
     2281 tgaagttcaa tatcgagatc gaatgcacgc cggaggaggc acggcacttc ctcggcctgc
     2341 ccgaggtggc gccgatgcag cagcggctga tcgaggagat cgaggcgcag ctcaagaaga
     2401 ccgtgcacga gatggacggc aaggcgatgc tcgagcaatg gctgccgatc gggatgaagg
     2461 gggtcgagca gttccagtcg atgtggaccc agctcgccgc cgcggcagcc ggggtccagc
     2521 gcaaccgcaa gccgagcgac gagggtggcg ccgacaagag ccgcaagccc tgaggacagg
     2581 ctgtcgatga acgagcggcg ggccacggtc tttgcaccgg ccaccccgcc cgggcgctcg
     2641 gcgctcggcg tcctgcggat ttccggcccg gccgcggcgc cggcggccga gcagctggcc
     2701 ggccgggtcc cggagccacg ccgggcaacg gtcgccgaac tgcgcgatcc ggcgagcggc
     2761 gccctgctcg atcgtgccct gctgctcttc tttccggcgc cgtcgagcag cacgggcgag
     2821 gacgtgctcg agatccagca tcacggcggc gccgcggtcg cccgttcgct ggtcgaggcg
     2881 ctcggcgcga tgccggagct gaggccagcc gagcccggcg agttcagccg ccgcgcgttc
     2941 ctggcaggca agctcgacct cacggctgcc gagggcctgg ccgacctgat cgacgcgacg
     3001 agcgccgccc aggcacgggc agcgctccgc cagatggatg gcgcgctcgg ggccctctat
     3061 gtggactggc gcgagcgcct cctgcgggcc ctcgccctgc tcgaggcgga gatcgacttc
     3121 gccgcggagg aggaggttcc ggacaggctc tgggcattcg tcgagccgga tctcgcggcc
     3181 cttgcggccg accttcgacg ccatctggac gacggccgac ggggtgagcg cctgcggcgc
     3241 ggcatcgcca tcgcggtgat cggcgccccc aatgtcggca agtcgagcct cgtcaacgcg
     3301 atcgcccggc gggacgtggc catcgtgaca cccttcgccg gcaccacgcg cgacgtcatc
     3361 gaggtggcgc tcgatctggc cagcctgccg gtgacgctgc tcgacacggc cgggctgcgc
     3421 gacagcgacg acccgatcga gctccaaggc atcgcccggg ccaaggcccg ggcggcgtcc
     3481 gccgatctca ggctgctggt ggtcgacgat cctgccgatc tgccggaagc cgaaggcaac
     3541 gatccgacca cgctccgcgt cctcaacaag ctcgatctgt gtcgtcgcca cgatttgctg
     3601 ccaggggtca ttggcgtctc ggccacaacc ggcgaggggc tggatggact gctggcagcc
     3661 gtcgctgccg tcgcccgcga cctgctgccg ggcacggacg aggtgctgct cacccgcgcc
     3721 cggcaccgca ccgctctgca cgatgcccaa aatgccctgg aacgggcgct ggcgctcggt
     3781 gtcgcgggcg atctcgggct gctggcggag gaggtccggc ttgccggccg tgcgctcggc
     3841 cgcatcaccg gcgctttcgg tgtcgaggac atcctggatc gggtgttcag cgagttctgc
     3901 atcggcaagt gacgctgttt cacgtgaaac agcgccgctg tggggccatc agccggcgga
     3961 ggtccgttcg gcgtgccgcc gggtcgccac ctcgcgaacc cggcggccga gcacgctgtg
     4021 actgcccatc acctgctcga acagctcgcg acgcaggatc agcaggcggc agaagccgat
     4081 ggcgaccaca tcggcattgc ggggtcgccg ggtcagcagc gccagctcgc cgaagaagtc
     4141 gcccgtcccg agccgcaccg gctcgggctg gacacgcacc tcgacggcgc cggtggcgat
     4201 gaagaacatg gcgtcgcccg gctcgccgcg tcgcacgatc cgctcgccgg gcagggccag
     4261 ccgggtcttg agctgccgcg ccagctgctt gatctccttc tcgtccagct tggcgaagag
     4321 cggcatgtgc cggatgagct cggccgtacg caggccgaga tcgaggtgtg gcgggcgctc
     4381 gaggcgggtg cggcgctgcc gcagcgcctg gcccatcatg tgggcgacct gcggcgacag
     4441 cagccgctcc tgctccatct gctcgatcgc ggcatattcg agacgaagag ccgcgagcgc
     4501 cagatatcgc ttgccgaacg aggcccagta gtcggggtac tgcaggcgca tcgcgtcgat
     4561 cgcccgctcg acctggtcga ggcgggtttc cagaaaaaga cccgcaacct cgctgacgcg
     4621 ctcgccgaac aggccgcgaa tgcgctcggt attgaaggtc atcagctcgc tcagcacgta
     4681 gcgctgcacg atgtcgcgct cgagatgcag ggtcagccga cgggcgagag gcgtgtccca
     4741 gcgcagccgg cgatgcagga acgcggcaaa gcgggttgcc cggtcgaaac gcacctggtc
     4801 gcgcgcgagg tagcggtagc cctccagccc gtcggcgcgc agcgcatcga tcagggcgcc
     4861 gacctggcgg accagcgccg cattggccga gcgcgcgatc atgcgccggg cgcgctcctc
     4921 ggcatagagc tccgcctcac gccggctgag cgtcaggagc gccagaacca cctgtcgccg
     4981 ctgcatctcc tcgcccaccg gccgggcgac ggccgctgca tcctgctgcc ggtagtcgtt
     5041 cgccaccgcc tcgacgctgg cggggtcgag catgcgctcg gccgccgcgt cgcgcagcgt
     5101 gtcgacgatc tcgccatggg tgagcaggat ggctcgctgc ctgaggattt cctcgagcgg
     5161 cgacaggcgg tcgaggccga ccaggcggat cagccagcgg agcgtggtcg cctggacgaa
     5221 gagggtgaac agcacgtagc cggtagccag caccgccacc agatgctgga cctcgtccgg
     5281 caccagcggg ttctcggtga ccgcgagggc gaggg
//
LOCUS       DAOTFI010000291         5309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_18410_length_5309_cov_9.072136, whole genome shotgun sequence.
ACCESSION   DAOTFI010000291 DAOTFI010000000
VERSION     DAOTFI010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18410_length_5309_cov_9.072136"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="P5558_21320"
     CDS             complement(<1..752)
                     /locus_tag="P5558_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009827704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRY26924.1"
                     /translation="MAGQRQHDYHILPPSIWPFIGAAGGFTLLFGLVLLFHGNGPWVA
                     MIGLVTVLYTMFCWWSDVIAEARIGDHTPVVQIGLRYGMIMFIVSEIMFFSAWFWSFF
                     KNALYPMAAIDHTWPPPGIETFDPWHLPLINTLILLCSGCAATWAHHALVHENDRRQM
                     RWGLWLAIVLGLLFTTFQIFEYSHAQFSFAGNVYGANFFMATGFHGFHVIIGTIFLAV
                     CLYRVYAGDFTPTQHVGFEAAAWYWHFVDVVWL"
     gene            complement(765..1361)
                     /locus_tag="P5558_21325"
     CDS             complement(765..1361)
                     /locus_tag="P5558_21325"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRY26925.1"
                     /translation="MRPSPNGRIALGLGGVVAVMVALSFAAVPFYDWFCRVTGYAGTT
                     SVAEAPAGKILDRKVTVRFDANTAPDMPWVFRPMQRTMEIRLGETGLALYEAYNPTDR
                     TIGGRASYNVAPYSVGGYFDKIACFCFNEQVLGPGERVEMPVSFYVDPAMLDDREARD
                     VTGITLSYTMHRAELPEPEEAPAAQAALATGGDGTTKQ"
     gene            complement(1366..1551)
                     /locus_tag="P5558_21330"
     CDS             complement(1366..1551)
                     /locus_tag="P5558_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase assembly protein"
                     /protein_id="HRY26926.1"
                     /translation="MRPDSHEIFGRRRSRNIGLGLVLGAFVLLVFAVTMVKLSEGQSM
                     QGFDHTLRPSLLPPAEE"
     gene            complement(1563..2519)
                     /gene="cyoE"
                     /locus_tag="P5558_21335"
     CDS             complement(1563..2519)
                     /gene="cyoE"
                     /locus_tag="P5558_21335"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRY26927.1"
                     /translation="MSDTDWTLAARAHEPAFGDYIELLKPRVMRLVVFTAFVGMVCAP
                     VAVHPVIGLASILCIAVGAGAAGALNMWQDWDIDAVMRRTAARPIPSGRVERGEALAI
                     GLTLAAFAVVMLALFANPFAAGLLAFTIFFYAVVYSIWLKRATPQNIVIGGAAGAFPP
                     MIGWAVATGGIAPESVLMFLLIFVWTPPHFWALALFRNDDYRRAGVPMLPVTAGDRVT
                     RNHILAYSLVLAPIAIAPAFTAIGGPVYLAGAVVLDALLLVGAARLWRRDPDSASRDR
                     FLAEKRFFGLSIVYLFLLFALLLAEAALGAAGLTPAGWPVLV"
     gene            complement(2527..3735)
                     /gene="coxB"
                     /locus_tag="P5558_21340"
     CDS             complement(2527..3735)
                     /gene="coxB"
                     /locus_tag="P5558_21340"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRY26928.1"
                     /translation="MTTRIGAALGFAALWAATGAFAQGEPAGLPIIGAPVPGGTGYQP
                     ASSPVAEDMHWLSHWVHGIMFGVVLLVMVLLAIVVLRFNRRSNPTAARFTHNSRLEIA
                     WTLLPVLILILIGSFSLPVLFKQLEVPEPDLTIKVTGNQWYWSYEYPDYEISFDSLLL
                     PREDLEAHGYSDEYYLLAADNSVVVPVGAVVELLVTGSDVIHSWTIPAFGVKMDAVPG
                     RLADTWFSANQEGIYFGQCSELCGKDHAYMPITVKVVSQEAYDAWLDWAIDEYGGTRP
                     EAVAAAPAAGAAAAAETGEPAVEVEAGAAAEATVEEEAAADDANLDAEAGDAESGAAA
                     EEPAAEATEAEAPAEPAVEEEAGAATEATADEEAAADDANADAEAGDAESDAAAEEPA
                     SEDAPAEPAH"
     gene            3901..>5309
                     /gene="tldD"
                     /locus_tag="P5558_21345"
     CDS             3901..>5309
                     /gene="tldD"
                     /locus_tag="P5558_21345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HRY26929.1"
                     /translation="MPSSPRFRPFDDNLDADRALAILRSATDGADDGELFLERRRGEA
                     LVFDDGRLRNASYDAAEGFGLRAVRGEATGYAHATEISEASLRRAAETARIAVGSGGG
                     RLAAAPRPSNQRLYSDHDPFDDAAFAVKVELLREIDDFLRGLDPRVVQVTASLAAAMQ
                     EVEILRPEGGRLAEARPMVRLNVSVIVEENGRRESGSAGGGGRHGLAPLMAREHWQGV
                     AREALRIAAVNLRAEPAPAGVMDVVLGPGWPGILLHEAVGHGLEGDFNRKKTSAFAGL
                     MGSRVAAPGVTVVDDGTLPDRRGSISFDDEGTPSGRNVLIEDGILVGFMQDRQNARLM
                     GVPATGNGRRESYAHAPMPRMTNTIMLGGETDPKDVLAGLGNGIYAVGFGGGQVDITN
                     GKFVFSCTEAYRVKDGKVGAPVKGATLIGDGATALQKIRAIGNDMALDPGMGNCGKNG
                     QWVPVGVGQPTLLIGGLTVG"
BASE COUNT          865 a         1806 c         1910 g          728 t
ORIGIN      
        1 agccagacca cgtcgacgaa gtgccagtac caggccgccg cctcgaagcc gacatgctgg
       61 gtcggggtga agtcgcccgc atagacccgg tagaggcaga ccgcgaggaa gatcgtcccg
      121 atgatgacgt ggaagccgtg gaagccggtc gccatgaaga agttggcgcc gtagacattg
      181 cccgcgaagc tgaactgggc gtggctgtac tcgaagatct ggaaggtcgt gaacagcagg
      241 ccgagcacga tcgcgagcca gaggccccag cgcatctgcc ggcggtcgtt ctcgtggacc
      301 agcgcatggt gcgcccaggt cgccgcgcag cccgagcaga gcaggatcag cgtgttgatc
      361 agcggcaggt gccaggggtc gaaggtctcg atgccgggcg gcggccaggt gtggtcgatc
      421 gccgccatcg ggtagagcgc gttcttgaag aacgaccaga accaggcgga gaagaacatg
      481 atctccgaga cgatgaacat gatcatcccg tagcgcagcc cgatctggac cacgggcgtg
      541 tggtcgccga tccgggcctc ggcgatcacg tccgaccacc agcagaacat ggtgtagagg
      601 accgtcacca ggccgatcat cgccacccag ggcccgttgc cgtggaagag cagcaccagc
      661 ccgaagagca gggtgaagcc gccggcggcc ccgatgaagg gccagatgct cggaggcagg
      721 atgtgatagt cgtgttgcct ttgtccggcc atgacccgtt ccctctattg tttcgtcgtg
      781 ccgtcgccgc cggtcgcgag cgccgcctgc gccgccggcg cttcctccgg ttcgggcagt
      841 tccgcccgat gcatggtata ggacagggta atccccgtca cgtcgcgcgc ttcgcggtcg
      901 tccagcatcg cgggatcgac atagaacgac accggcatct cgacccgctc gcccggcccg
      961 agaacctgtt cgttgaagca gaagcaggcg atcttgtcga aatagccgcc gacggaatag
     1021 ggggccacgt tgtagctcgc cctgcccccg atcgtcctgt cggtgggatt ataggcctcg
     1081 tagagcgcaa ggcccgtctc gccgaggcgg atctccatgg tgcgctgcat cggccggaag
     1141 acccagggca tgtcgggcgc ggtattggcg tcgaagcgca cggtgacctt gcggtcgagg
     1201 atcttgcccg ccggcgcctc ggccaccgag gtcgtcccgg catagccggt gacgcggcag
     1261 aaccagtcgt agaagggcac cgccgcgaag ctcagcgcga ccatcaccgc gacgacgccg
     1321 ccgaggccga gcgcgatcct gccattgggg gacggccgca tccgctcact cctccgccgg
     1381 tggcagcagc gagggccgca gggtgtgatc gaagccctgc atggactggc cttcgctgag
     1441 cttgaccatg gtcaccgcga agacgagcag cacgaaggcg ccgagcacca ggcctaggcc
     1501 gatgttgcgg ctgcggcggc gtccgaagat ctcgtggctg tcggggcgca tcccgccgcc
     1561 cctcagacca ggaccggcca gccggccggg gtcaggccgg cggcgccgag cgcggcttcc
     1621 gcgaggagca gcgcgaagag caggaagaga tagacgatcg agaggccgaa gaagcgcttc
     1681 tcggccagga agcggtcgcg ggacgccgaa tccgggtcgc gccgccagag ccgcgccgcg
     1741 ccgacgagca ggagcgcgtc gagcacgacc gccccggcga gatagaccgg gcccccgatc
     1801 gcggtgaagg ccggcgcgat ggcgatcggc gcgaggacca gggaataggc gaggatgtgg
     1861 ttccgggtga cgcggtcgcc cgcggtcacc ggcagcatcg gcacgccggc gcggcggtag
     1921 tcgtcgttgc ggaagagcgc cagcgcccag aagtgcggcg gggtccagac gaagatcagc
     1981 aggaacatca gcacgctctc gggcgcgatg ccgccggtcg ccaccgccca gccgatcatc
     2041 ggcgggaagg cgcccgccgc accgccgatg acgatgttct gcggcgtcgc gcgcttgagc
     2101 cagatcgaat agaccaccgc gtagaagaag atggtgaagg ccaggagccc cgcggcgaag
     2161 ggattggcga agagcgcgag catcaccacc gcgaaggcgg ccagcgtcag cccgatcgcc
     2221 agcgcctcgc cgcgctcgac ccggccggag gggatcggcc gcgcggcggt gcgccgcatc
     2281 accgcgtcga tgtcccagtc ctgccacatg ttgagcgcgc ccgcggcgcc ggcgccgacg
     2341 gcgatgcaga ggatcgacgc cagtccgatc accggatgca ccgcgaccgg ggcgcagacc
     2401 atgccgacga aggcggtgaa gaccaccagc cgcatcaccc gcggcttcag gagctcgatg
     2461 taatccccga aggcgggctc gtgcgcccgc gccgcgaggg tccagtcggt gtcgctcatc
     2521 gcgccctcag tgggccggct cggccggagc gtcttcggaa gcgggctcct cggccgcggc
     2581 gtcgctttcg gcgtcaccgg cctcggcgtc ggcattggcg tcgtcggcgg cggcctcctc
     2641 gtcggcggtc gcctcggtgg cggcgccggc ctcctcctcg accgcaggct cggccggcgc
     2701 ttcggcttcg gtcgcctccg cggccggttc ttccgcggcg gcaccgcttt ccgcgtcgcc
     2761 ggcctcggcg tcgagattgg cgtcgtccgc ggcggcttcc tcctcgacgg tcgcctcggc
     2821 ggcggcaccg gcctcgacct cgacggctgg ctcgccggtc tccgccgcgg ccgcggcgcc
     2881 ggctgccggc gctgcggcga ccgcctcggg ccgggttccg ccgtattcgt cgatcgccca
     2941 gtcgagccag gcgtcatagg cctcctggct cacgaccttg acggtgatcg gcatgtaggc
     3001 gtggtccttg ccgcagagct cggagcactg gccgaaatag atgccctcct gattggcgga
     3061 gaaccaggta tcggcgaggc ggccggggac cgcgtccatc ttcacgccga aagccgggat
     3121 ggtccaggaa tggatcacgt cggagccggt caccaggagc tcgaccaccg cgcccacggg
     3181 gaccacgacc gaattgtccg cggccaggag ataatattcg tccgaatagc cgtgcgcttc
     3241 gagatcctcg cgcggcagca gcaggctgtc gaaggagatc tcgtagtcgg gatattcgta
     3301 gctccagtac cactggttgc ccgtcacctt gatggtcagg tcgggctccg gcacctcgag
     3361 ctgcttgaag agcacgggca gcgagaagga gccgatcagg atgaggatca gcaccggcag
     3421 cagcgtccag gcgatctcga gccgggaatt gtgggtgaag cgcgccgcgg tggggttcga
     3481 ccggcggttg aaccgcagca cgacgatcgc caggagcacc atcaccagca ggacgacgcc
     3541 gaacatgatc ccgtgcaccc agtgcgacag ccagtgcatg tcctcggcga ccgggctcga
     3601 agccggctgg tagccggttc caccgggcac cggcgccccg attatcggga ggcccgcggg
     3661 ctctccctga gcgaaggcgc ccgtcgccgc ccagagcgca gcgaagccca gcgcggcacc
     3721 gatccgtgtg gtcatcttca tcgtcccatc ccgccgctgc ggcgccggcc ccccgacgcg
     3781 aggtatgcgg ctgtgttgca gccgccggca ggtgaaccat attagagcct gaagaacaag
     3841 atgcggcgtt gccgagccgg ccagttcgtt cgcccgcccc acccgacccg gagaccatcc
     3901 atgccatcct ccccgcgctt ccggcccttc gacgacaatc tcgacgccga ccgggccctg
     3961 gcgatcctgc gcagtgccac cgatggggcg gacgacggcg agctcttcct cgagcggcgc
     4021 cggggcgagg cgctggtctt cgacgacggg aggctccgga acgcgagcta cgacgcggcc
     4081 gagggcttcg ggctgcgcgc cgtgcgcggc gaggccaccg gctatgccca tgcgaccgag
     4141 atctccgagg cgagcctccg gcgcgcggcc gagaccgccc ggatcgcggt cgggagcggc
     4201 ggcggccggc tcgcggcggc gccgcggccg agcaaccagc ggctctattc cgatcacgac
     4261 cccttcgacg acgcggcatt cgcggtgaag gtggagctgc tgcgcgagat cgacgatttc
     4321 ctgcgcggcc tcgatccgcg cgtggtccag gttaccgcga gcctcgcggc ggcgatgcag
     4381 gaagtcgaga tcctgcgccc cgagggcggt cgcctcgccg aggcccggcc gatggtgcgg
     4441 ctcaacgtct cggtcatcgt cgaggagaac ggccggcggg aatcgggctc ggccggcggc
     4501 ggcggccgcc acgggctcgc gccgctcatg gcaagggaac attggcaggg cgtcgcccgc
     4561 gaggcgctgc gaatcgccgc ggtcaacctg cgcgccgagc cggcgccggc aggcgtcatg
     4621 gacgtggtgc tcggccccgg ctggcccggc atcctgctgc acgaggcggt cgggcacggg
     4681 ctggagggcg atttcaaccg caagaagacc tccgccttcg ccgggctcat gggcagccgc
     4741 gtggcggcgc cgggggtgac ggtggtcgac gacggcacgc tgcccgaccg ccgcggctcg
     4801 atcagcttcg acgacgaggg cacgccatcg ggccgcaacg tgctgatcga ggacgggatc
     4861 ctcgtcggct tcatgcagga ccggcagaac gcgcgcctga tgggcgtgcc ggcgacgggc
     4921 aacggccggc gagaaagcta cgcccatgcc ccgatgccgc gcatgaccaa cacgatcatg
     4981 ctcggcggcg agaccgatcc gaaggacgtg ctcgccggtc tcgggaacgg catctatgcc
     5041 gtcggattcg gcggcggtca ggtcgacatc accaacggca agttcgtctt ctcctgcacc
     5101 gaggcctatc gggtgaagga cggtaaggtc ggcgcgccgg tcaagggcgc gaccctgatc
     5161 ggcgacggcg ccacggcgct gcagaagatc cgcgccatcg gcaacgacat ggcgctcgac
     5221 ccgggcatgg gcaattgcgg caagaacggc cagtgggtgc cggtgggcgt cggccagccg
     5281 acgctgctga tcggcgggct gaccgtggg
//
LOCUS       DAOTFI010000292         5303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_18446_length_5303_cov_5.326791, whole genome shotgun sequence.
ACCESSION   DAOTFI010000292 DAOTFI010000000
VERSION     DAOTFI010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18446_length_5303_cov_5.326791"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="P5558_21350"
     CDS             <1..799
                     /locus_tag="P5558_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26930.1"
                     /translation="ERIRVSLGLDRPFLERFGEWSWRILHADLGTSMFTGLPVTKMIA
                     QRLEPTLSLMLITLVLSIAIAVPIGVVAAWKAGSWLDRSLMGFAVFGFSVPVFVVGYL
                     LAYVFALELDWLPVQGYTPLGDGLWPWLENLILPAIALGCVYIALIARITRASMLEVL
                     QQDYIRTARAKGLAQRPILFVHALKNAAVPIVTVIGIGVALLIGGAVVTESVFAIPGL
                     GRLTIDAILRRDYPVIQGIVLLFSFVYVLVNLLVDLVYTTLDPRIRY"
     gene            965..1876
                     /locus_tag="P5558_21355"
     CDS             965..1876
                     /locus_tag="P5558_21355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY26931.1"
                     /translation="MLAPLLGTVDPAALAPSKRTRPPSVDYWFGSDMLGRDIDEIRAD
                     GMTELGGGQRDRSGEVLLEAKAAATDRRLHRLDLIVHRGEIVGLAGLLGSGRTEAARA
                     VFGLDRLQKGSVSFKGQAPSDPASAIAAGIGYLTEDRKAEGIVPEMSVRENVTLAVLP
                     KLSRGGRIERGRERALVEAFIKSLGIKTANMDQPIRELSGGNQQKALLARWLATEPEL
                     LILDEPTRGVDVGAKLEIQAIIRDYVARGFGVLLISSEFEELVEGADRITVMQDGYSV
                     TELQNPGITENALVKAIAHHHRGQAPA"
     gene            1873..2874
                     /locus_tag="P5558_21360"
     CDS             1873..2874
                     /locus_tag="P5558_21360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26932.1"
                     /translation="MSTRLESASPATASPATARALRQLRRHGGLVALLVLVLVNIAIT
                     PNFLQLQTLFVNISQVATIAIVAIGMTLVIATGGIDLSVGAVMALAGALAPLIFLSPF
                     GVAYPALGLVAAFTLPLLAAMACGLFNGFMVSVLRVQPIIATLILFISGRGIAQVLTN
                     GNLQTFSNPSFSYLGTGRILGLPFQGWLALALAGLVYWLVTRTLFGRYLLAIGGNERA
                     ARLSGVPVRRVKFGAYVICAGLAGIAGLIVVAINSASDAGRNGNLMELDAIAAAVVGG
                     ALLQGGRAPIFGAVLGAVIIQLVKYTLLANGVEDEVALIAKAFIIVVAVYLQAGRRE"
     gene            2877..3872
                     /locus_tag="P5558_21365"
     CDS             2877..3872
                     /locus_tag="P5558_21365"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY26933.1"
                     /translation="MGDATTFDLRRYVRGQAQSNAVWLALMALIAFGLLRYDNFGGAY
                     NIVSFLNYNSMFIVISIGMCFVIMTGGIDLSVGSVAAFTSVVAAYLSPWGIEVALPGG
                     ILAGIAFGAVNAFVIILMRVPPFIATLATMLAAKGGALVLSGAQTISVDWGSNFTKLG
                     MEKAFGVVPWTIVLVAIVALAAWFVLERTSLGRTVLAIGGGEDASVLMGLEVDRTKAF
                     VYMLSGACAGIAGVFLASGFGAGQPLEGVGWELSAIASVVVGGTLLTGGLGSVPATVA
                     GALLLGLVFNILNFENGRGTISLSAYWQLVVRGGFLFIVILLQNRVSSARAAEDH"
     gene            3874..4965
                     /locus_tag="P5558_21370"
     CDS             3874..4965
                     /locus_tag="P5558_21370"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY26934.1"
                     /translation="MPLIETIEARLFRVPLDETLADAKHGDHTHFELITATIRLDDGS
                     EGTGYSYTGGKGGRAVLAMIEHDLAPFLIGHDAGPVEDLHDAMQWHIHYVGRGGIASF
                     AISALDIALWDLRGKNRGQALWQMAGGHSRRARAYCGGIDLGFPLPKLLESVQGYLDR
                     GFDGVKIKVGQPTLAEDVERVAAVRELIGPKIAFMVDANYSMSVDQAIRAAEAFAPFD
                     LLWFEEPIIPEDFDGYARIAEATGMPLAQGENLHTLHEFEAAIAGAKLAYIQPDASNC
                     GGITGWLAVADMAEDHGIPACSHGMQELHVSLVAGHPNGGWIEVHSFPIDRYTKHPLA
                     TLHHRAVAPEEHGTGVTFDWDKLAPYLVR"
BASE COUNT          784 a         1977 c         1626 g          916 t
ORIGIN      
        1 cgagcgcatc cgggtgagtc tcggcctcga ccgaccgttt ctggaacgtt tcggcgagtg
       61 gtcgtggcgc atcctgcatg ccgacctcgg cacctcgatg ttcacgggcc tgcccgtgac
      121 gaagatgatc gcgcagcggc tcgagcccac gctctcgctg atgctgatca ccctcgtgct
      181 ctcgatcgcg atcgcggtgc cgatcggtgt cgtggcggcc tggaaggccg gcagctggct
      241 cgaccggtcg ctgatgggct ttgccgtgtt cggcttctcg gtcccggtgt tcgtcgtggg
      301 ttacctgctg gcctacgtct tcgcgctgga actcgattgg ctgccggtcc agggctacac
      361 gccgctgggc gacgggctct ggccgtggct ggagaacctg atcctgcctg caatcgcgct
      421 cggctgcgtc tacatcgcgc tgattgcacg aattacacgc gcgtccatgc tcgaggttct
      481 gcagcaggac tacatccgca cagccagggc gaaggggctg gcgcagcgcc ccatcctctt
      541 cgtccacgcc ctgaagaacg ccgccgttcc gatcgtgacc gtcatcggca tcggcgtggc
      601 cctgctgatc ggcggcgccg tcgtgaccga aagcgtcttc gcgatcccgg gccttggccg
      661 gctcacgatc gacgcgatcc tgcgccgcga ctacccggtc atccagggca tcgtgctgct
      721 gttcagcttc gtctacgtgc tcgtcaatct gctggtcgac cttgtctaca ccacgctcga
      781 tccgaggatc agatattgac cattacaccc cccaccgcat tcggcgccat gcagcttgcc
      841 ggcgaaccgg agcttcccga catcctgccg cccgtccgcg agcggcacgg catcagcggc
      901 ttcgtctacc gctatcccga tcgccatcgg cggcctgctg ctcacgctcc tggtggcgat
      961 ggccgtgctc gcgccgttgc tcggcacggt cgatcccgcg gcactcgccc cctccaagcg
     1021 tacccgccca ccgtccgtcg actattggtt cggctcagac atgctcggcc gcgacatcga
     1081 cgagatccgc gccgacggca tgacagagct cggcggcggc caacgggacc ggagcggcga
     1141 ggtgctgctc gaggcgaagg cggccgccac cgaccggcgc ctccaccggc tcgacctcat
     1201 cgtccaccgg ggcgagatcg tgggcctcgc cgggctcttg ggctccggcc gcaccgaggc
     1261 ggcccgcgcc gtctttggcc tcgaccggct gcagaagggc agcgtcagct tcaaaggcca
     1321 ggccccctcg gacccggctt ccgccatcgc cgccggcatc ggctacctca ccgaggaccg
     1381 caaggccgag ggcatcgtgc ccgagatgtc ggtgcgcgag aacgtgacgc tggcggtgct
     1441 gccgaagctc agccggggcg ggcggatcga gcgcggccgg gaacgcgccc tcgtcgaggc
     1501 cttcatcaag tcgctcggca tcaagaccgc caatatggac cagccgatcc gcgagctctc
     1561 gggcggcaac cagcagaagg cgctgctcgc ccgctggctc gcgaccgagc cagagctttt
     1621 gatcctcgac gagccgaccc ggggcgtcga tgtcggcgcc aagctcgaga tccaggctat
     1681 catccgcgac tacgtcgccc gtggcttcgg cgtgctcctc atctcctcgg aattcgagga
     1741 gctggtcgag ggtgccgacc gcatcaccgt catgcaggac ggctactcgg tcaccgagct
     1801 ccagaacccc ggcatcaccg agaacgccct ggtcaaggcg atcgcccacc atcaccgggg
     1861 ccaggccccg gcatgagcac gcgcctcgaa tccgcaagcc cggccacggc gtcgcccgcg
     1921 accgcccggg cccttcgcca gctccgccgc catggcgggc tggtcgccct tctggttctc
     1981 gtcctcgtca acatcgccat cacgcccaac ttcctccagc tgcagacgct cttcgtcaac
     2041 atcagccagg tcgcgaccat cgccatcgtc gccatcggca tgaccctggt gatcgcgacc
     2101 ggcggcatcg acctctcggt cggcgccgtc atggcgctcg cgggcgcgct cgcccccttg
     2161 atcttcctct cgcccttcgg cgtcgcgtac cctgccctcg gcctcgtcgc cgccttcacg
     2221 ctgccgctgc tggcggccat ggcctgcggc ctcttcaacg gcttcatggt gagcgtgctc
     2281 cgggtccagc cgatcatcgc caccctcatc ctcttcatct ccggccgcgg catcgcccag
     2341 gtgctgacca acggcaacct gcagaccttc agcaatccct ccttcagcta tctcggcacc
     2401 ggcaggatcc tcggcctgcc cttccagggc tggctggcgc tggcgctcgc cgggctcgtc
     2461 tactggctcg tcacccgcac cctcttcggc cgctatctcc tcgccatcgg cggcaacgag
     2521 cgggccgccc ggctctccgg cgtgccggtg cgccgcgtca agttcggcgc ctacgtcatc
     2581 tgcgccgggc tcgccggcat cgccgggctg atcgtcgtcg ccatcaactc ggcctcggac
     2641 gccggccgca acggcaacct catggagctc gacgccatcg ccgccgcggt ggtcggcggt
     2701 gcgctcctgc agggcggccg tgctcccatc ttcggcgccg tgctgggtgc cgtgatcatc
     2761 cagctcgtca agtacacgct gctcgccaac ggcgtggagg acgaggtggc gctgatcgcc
     2821 aaggccttca tcatcgtcgt cgccgtctac ctgcaggccg ggcggcggga gtagccatgg
     2881 gcgacgccac caccttcgac cttcgccgct acgtccgggg ccaggcccag agcaacgccg
     2941 tctggctggc gctcatggca ctcatcgcct tcggcctcct gcgctacgac aatttcggcg
     3001 gcgcctacaa catcgtctcc ttcctcaact acaactcgat gttcatcgtc atctccatcg
     3061 gcatgtgctt cgtcatcatg accggcggca tcgacctctc ggtcggctcg gtcgcagcct
     3121 tcacctcggt ggtcgcggcc tatctcagcc cctggggcat agaggtggcg ctccccggcg
     3181 gcatcctcgc cggcatcgcc ttcggcgccg tcaacgcctt cgtcatcatc ctcatgcgcg
     3241 tgccgccctt catcgccacg ctcgccacca tgctcgccgc caagggcggg gcgctcgtgc
     3301 tcagcggggc ccagaccatc tcggtcgact ggggcagcaa cttcacgaag ctcggcatgg
     3361 agaaggcctt cggcgtcgtg ccctggacca tcgtgctggt cgccatcgtc gccctcgccg
     3421 cctggttcgt cctcgagcgc acctcgctcg gccgcaccgt gctcgccatc ggcggcggcg
     3481 aggacgcctc cgtcctgatg ggcctcgagg tcgaccgcac caaggccttc gtctacatgc
     3541 tgagcggcgc ctgcgccggc atcgccggcg tttttttggc ctccggcttc ggggcaggcc
     3601 agccgctcga aggggtgggc tgggagctct cggccatcgc gtccgtcgtc gtcggcggca
     3661 cgctcctgac gggcgggctc ggctcggtcc ccgccaccgt cgccggtgcc cttttgctcg
     3721 gcctcgtctt caacatcctg aacttcgaga acggccgcgg caccatctcg ctcagcgcct
     3781 actggcagct cgtggtgcgc ggcggctttc tcttcatcgt catcctgctg cagaaccggg
     3841 tgagcagcgc cagagctgca gaggatcact gatatgccgc tgatcgagac catcgaggcc
     3901 cgcctcttcc gcgtccccct cgacgagacg ctggccgacg cgaagcacgg cgaccacacc
     3961 catttcgagc tgatcaccgc caccatccgc ctcgacgacg gcagcgaggg caccggctac
     4021 agctacaccg ggggcaaggg cggccgcgcc gtcctcgcca tgatcgagca cgacctggca
     4081 cccttcctca tcggccacga cgccggcccg gtcgaggacc tgcacgacgc catgcagtgg
     4141 cacatccact atgtcggccg gggcggcatc gcatccttcg ccatctccgc cctcgacatc
     4201 gccctttggg acctgcgcgg caagaaccgg ggccaggccc tctggcagat ggccggcggc
     4261 cacagccgcc gggcccgcgc ctattgcggc ggcatcgacc tgggcttccc cctgccgaag
     4321 ctgctcgaga gcgtgcaggg ctacctcgat cgcggcttcg acggcgtcaa gatcaaggtc
     4381 ggccagccga ccctggcaga ggacgtcgag cgcgtcgccg ccgtgcgcga gctgatcggc
     4441 ccgaagatcg ccttcatggt cgacgccaac tactcgatga gcgtcgacca ggcgattcgt
     4501 gcggccgagg ccttcgcccc cttcgacctc ctctggttcg aggagccgat catccccgag
     4561 gatttcgacg gctacgcccg catcgccgag gcgaccggca tgccgctcgc ccagggcgag
     4621 aacctgcaca cgcttcacga gttcgaggcg gccatcgccg gggcgaagct cgcctacatc
     4681 cagcccgacg cctccaattg cggcggcatc accggctggc tcgccgtcgc cgacatggcc
     4741 gaggaccacg gcatccccgc ctgcagccac ggcatgcagg agctccacgt cagcctcgtc
     4801 gccggccacc ccaacggcgg ctggatcgag gtccactcct tccccatcga ccgctacacc
     4861 aagcaccccc tcgccaccct ccaccaccgc gccgtcgccc ccgaggagca cggcaccggc
     4921 gtcaccttcg actgggacaa gctggcgccg tatctggtgc gctgatcatc ggcggcgcca
     4981 aggcatgatg ccggcttccc acagggggtc ggtgtgcaaa aaagaggtca gcgactgtct
     5041 tggtctgtca agctgcttac gctgcccgcc atggcgttcc gtccctgagc atggcgttgg
     5101 aaaaagtcgg gacacgaaaa agtcgggaca cgaaccgact ttaacaatca aaaagtcggg
     5161 acacgaaccg actttaacaa tcggaaaaag tcggttcgtg aggcagttgc aaaacgccct
     5221 gaaggggcgg aattgctcgg tattgagcgg tgaagcgtgg catcgcccag ctccatccga
     5281 taggttgatg ttgtcaagac aga
//
LOCUS       DAOTFI010000293         5291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_18498_length_5291_cov_6.980328, whole genome shotgun sequence.
ACCESSION   DAOTFI010000293 DAOTFI010000000
VERSION     DAOTFI010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18498_length_5291_cov_6.980328"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="P5558_21375"
     CDS             complement(<1..522)
                     /locus_tag="P5558_21375"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY26935.1"
                     /translation="MRVALLGCVGVSLTQFRGRLIDAMVARGHEVIGCAADHDPRVVA
                     ELGARGARFRQVPLDRTGLNPLRDVGSVLGLRRIFDELRPDMLLAYTMKPMVYGALAA
                     RLARVGRIFTMVEGMGYAFADGSEGRRRFLRAVQQPLYRLAFSLSHATFVLNPDDRDL
                     LRRRRLASPRHELV"
     gene            complement(586..2844)
                     /locus_tag="P5558_21380"
     CDS             complement(586..2844)
                     /locus_tag="P5558_21380"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26936.1"
                     /translation="MTEPAVLVQGLPGPYLASARQAERLRRPFGVDTPAAETSLALLL
                     MPLWWHRWLMAAIVLAATAISAALAFTAERQFVAVAKLAVLSAPVDSGQNPLGAAERP
                     RRLDDQIEYLTSRPLAQRLVDTLDLQVDSEFVPPQVAGGGFVARLGDWFLRRAEAVTW
                     LEPLTDGLNGLRPTTAPSDDARRVLANEKAIEAFDERLQVSPQGTSSILAVAFRSNDP
                     LKAATIANRVVEQFIAEQTAERRSDVEQAAAHLQDRAAMLRDELRTAEDAITAYLRRS
                     GLSGEAAADQRLAVLQQRLLDNAAARATQARKVERLTRIMAQGEIAALVAELEDSLAV
                     QRLLDEETGLRQQITQLSATLGERHPELVALRNEAEAVRGRLRTELTRRVALERDELA
                     LLDDERRTLVELLESLEGRAVDASEARLELAELERRAAASRALYEEFLRGSMATSARL
                     SMVDPGVRLVQSAAVPTRPSSPNVKVMLVIGGIAGLLLGAAAAHLVEHRANTFRSPRD
                     VEASTGLATLALMPRIPAGRRYPRPLDYLRDRPLSLPAERLKGLLVATLGETAPIGSS
                     RVVMVTSSLPGEGKSTVALGLATLAAASGLRTLFLDADMRRSLFSVDPGMEARWGLVD
                     LATEPGRGTGRLLLDPRTRLRILTELAGPEIDPQRILQSTVMADVLTALRTRFELVVV
                     DTPPVLAVSDAQSLAPMVDAAVLVARLGKTRRDELLDVLGRLDLARAPLIGTVLQDAT
                     TLGNSASAYYTA"
     gene            complement(2908..4905)
                     /locus_tag="P5558_21385"
     CDS             complement(2908..4905)
                     /locus_tag="P5558_21385"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRY26937.1"
                     /translation="MARARSKPLVSLAHDVALAGVAFLVAMFLRLGAESLAAWGLTDL
                     GIAAAVAMLTAGIAFLLGRTHTQIWRFAAWREFVELARAVAFASLLFLIVMFVIDRAA
                     AVPRSVPIIQALVLVVLLCGARLAYQAARRRGLRLGAFDRLATPSAVLIWGADRDAAT
                     YLHSLLSDSRAPFRPVGVIDDTGHFHGRNLHGVPILGTSADLPAVVARLGRQGTSPQF
                     LIASRALDQRQRRRLLEQASGVGLGVVQIPRRVEVPTDGLDDLFSVERLTRRQPITLD
                     DDSVVGLVVGRRVLVTGAGGSIGSELVRQIVGLRAATIVMIDRGEFNLYAIEHEVAAE
                     ARANGVALAPRIVDIRDTAALRRVFAAHRPEVVFHAAALKHVPLCELNPIETILNNVH
                     GTRNVANAAAAVGALAMVHVSTDKAVNPTNVMGATKRIAELYCQSLDWAMARTGVAVT
                     RFMIVRFGNVLGSSGSVVPLFRRQVLAGGPVTVTHAEVTRYFMSIKEAVGLVLRASAA
                     GLQAERSDRGRIFVLDMGEPVRILDVARQVIRLAGLEPERDIEIEFTGLRPGEKLYEE
                     LFYPDEALVPTPSEGVLAALSRPLPLPQLCRRLDLLIDAARRHDEATARRLVAGLVPS
                     MAPGDPGGDHQPASSAAQDRPALAPRGQPVLAPTGMARLGA"
BASE COUNT          870 a         1810 c         1905 g          706 t
ORIGIN      
        1 gaccagctcg tgccggggcg acgccaggcg tcgtcggcgc agcaggtcgc gatcgtccgg
       61 gttgagcacg aaggtcgcat gggagagcga gaatgcgagc cgatagagcg gctgctggac
      121 cgcgcggaga aagcggcggc gcccctcgct gccgtcggcg aaggcgtagc ccatgccctc
      181 gaccatggtg aagatccgcc cgacccgggc gagccgggcg gccagggcac catagaccat
      241 cggcttcatc gtgtaggcga gcagcatgtc cgggcgcagc tcgtcgaaaa tccgccgcag
      301 cccgaggaca ctgcccacgt cacgcagcgg gttgagcccc gtccggtcga gcggcacctg
      361 ccggaaccgg gcgccacgcg caccaagctc ggcgaccacc cgcgggtcgt ggtcggcggc
      421 gcaaccgatg acctcatggc cgcgggcgac catggcgtcg atcagccgac cgcgaaactg
      481 ggtcaggctg accccgacac acccgagcag ggcaacacgc aagatggaac ctccgaggcg
      541 tagagccgat cccccggccg gcatggtccg gccggggggt ggcagtcagg cggtgtagta
      601 ggcgctggcc gagttgccca gcgtcgtggc gtcctgcagg accgtgccga tcagaggtgc
      661 gcgggcgaga tccagccggc cgagcacgtc aaggagctcg tcgcgccgcg tcttgccgag
      721 ccgggcgacg agcaccgcgg cgtccaccat cggcgccaga gactgggcgt cggagacggc
      781 cagcaccggc ggcgtgtcga cgaccacgag ctcgaagcgg gtgcggagtg ccgtcagcac
      841 gtcagccatg acggtcgatt gcaggatgcg ctgcggatcg atctccggcc ccgccagctc
      901 ggtcaggatg cgcagacggg tgcgcggatc gagcagcagg cgacccgtgc cgcggcccgg
      961 ctcggtggcg agatcgacca gcccccaacg tgcctccatg cccggatcga cggagaacag
     1021 cgaccggcgc atgtcggcgt cgaggaagag cgtgcgcagg ccgctggcgg cggccagggt
     1081 ggcgagcccg agggcgaccg tgctcttgcc ttcgcccgga aggctggagg tgaccatcac
     1141 cacccggctg ctgccgatgg gcgcagtctc gccgagtgtg gcgaccagga gacccttgag
     1201 ccgttccgcg ggcagcgaga gcggccggtc gcgcagatag tcgagcggcc tcggatagcg
     1261 tcgacccgcc gggattcggg gcatgagcgc cagggtggcg agcccggtcg aggcctcgac
     1321 gtcgcggggg ctgcgaaagg tgttggcccg gtgctcgacc agatgcgccg cagcggcacc
     1381 gagcaggagg ccggcaatgc cgccgatcac cagcatgacc ttgacgttgg gagagctcgg
     1441 ccgggtcggg acagccgcac tttgaacaag ccgcacgcca gggtcgacca tggagagccg
     1501 ggccgaggtc gccatcgagc cgcgcagaaa ctcctcgtag agagcccggc tggcggccgc
     1561 gcgacgctcc agctcggcga gctcgagccg cgcctcgctg gcgtcgaccg ccctgccctc
     1621 gagcgattcg agcagctcga cgagggtgcg gcgctcgtcg tcgagcaggg ccagctcgtc
     1681 gcgctcgagc gccacgcggc gggtgagctc cgtacgcagc cggccgcgga ccgcctcggc
     1741 ctcgttgcgc agggcgacca gctcggggtg ccgctcgccg agcgtcgccg acaattgcgt
     1801 gatctgctgg cgcagtccgg tttcctcgtc gagcagccgc tggacggcca gcgagtcctc
     1861 cagctcggcg accagcgccg cgatctcgcc ctgggccatg atccgggtca gccgttcgac
     1921 cttgcgcgcc tgggtggccc gggccgcggc attgtcgagc agtcgctgct gcagcacggc
     1981 gagacgctgg tcggcggccg cttcgccgct gaggccggag cgccgcaagt aggccgtgat
     2041 ggcgtcctcg gcggttcgca gctcgtcgcg gagcatggct gcccggtcct ggaggtgagc
     2101 cgcagcctgc tcgacgtcgg agcgccgctc ggctgtctgc tcggcgatga actgctcgac
     2161 gacgcgattg gcgatggtcg ccgccttcag gggatcgttc gagcggaagg cgacggcgag
     2221 aatcgaggag gtgccctgcg ggctgacctg gaggcgttcg tcgaacgcct cgatcgcttt
     2281 ctcgttggcc agcacgcgcc gcgcatcgtc gctgggcgct gtggtcggcc ggagcccgtt
     2341 cagcccatct gtcaacggct cgagccaggt gaccgcctcg gcccgccgca ggaaccagtc
     2401 gcccaacctg gcgacgaagc caccgccggc aacctgcggc gggacgaact cgctgtccac
     2461 ctgaaggtcg agcgtgtcga ccaggcgctg cgccagcggc cgggaggtca gatactcgat
     2521 ctggtcgtcg agccgccgcg gccgttccgc cgcgcccagc gggttctggc ccgaatcgac
     2581 cggtgcgctg agcacggcga gcttggccac ggccacgaac tgccgctcgg cggtgaaggc
     2641 caaagcggcg ctgatcgccg tcgccgccag gacgatggcg gccatcagcc agcggtgcca
     2701 ccagagcggc atgagcagca gagcgaggct ggtctccgct gccggtgtgt cgacaccaaa
     2761 cggccggcgc agccgttccg cctggcgcgc ggacgccaga taggggccgg gcaggccttg
     2821 cacgaggaca gctggttcag tcatcggcgt tgacctcacg cgaaagccgt agagatgccc
     2881 caggcacgga gctggcggat tcggccgtca tgcgccgagc cgcgccatgc cggtgggtgc
     2941 cagcaccggc tgccctcggg gcgccagcgc cggccggtcc tgagcagcgc tcgaggcggg
     3001 ctggtggtcg cccccgggat cgcccggcgc catgctcggc accagcccgg cgacgagccg
     3061 gcgggcggtt gcctcatcgt gacgtcgggc ggcgtcgatc agcaggtcga gccggcggca
     3121 gagctgcggc agcggcagcg gccgcgagag cgctgccagc acaccctcgg acggcgtcgg
     3181 aacaagggcc tcgtccgggt agaacagctc ctcgtagagc ttctcgccag gcctgagacc
     3241 ggtgaactcg atctcgatgt cgcgctccgg ctccagcccg gcgaggcgga tcacctgcct
     3301 ggcgacatcg aggatgcgca ccggctctcc catatcgagg acgaagatcc ggccacggtc
     3361 ggagcgctcg gcctgcaggc cggcggccga ggcgcgcagg acgaggccga cggcttcctt
     3421 gatcgacatg aagtagcggg tcacctcggc gtgggtgacc gtcaccggtc cccctgccag
     3481 cacctggcgg cggaacaggg ggacgaccga gccgctcgag ccgaggacat tgccgaaacg
     3541 cacgatcatg aagcgggtga cggcgacgcc ggtccgggcc atcgcccagt cgaggctctg
     3601 gcagtagagt tcggcgatcc gcttggtggc acccatcacg ttggtcgggt tgaccgcctt
     3661 gtcggtcgag acatggacca tggcgagcgc gccgaccgcc gcggcggcgt tggcgacgtt
     3721 gcgggtgccg tgcacgttgt tgaggatcgt ctcgatcggg ttgagctcgc agagcggcac
     3781 atgcttgagc gccgccgcgt ggaacacgac ctcgggtcga tgggcggcga acacgcgacg
     3841 cagcgctgcc gtgtcgcgga tgtcgacgat ccgcggcgcc agcgccacgc cgttggccct
     3901 ggcctccgcg gcaacctcgt gctcgatagc gtagaggttg aactcgccgc gatcgatcat
     3961 cacgatggtc gccgcacgca ggccgacgat ctggcggacc agctcgctgc cgatgctacc
     4021 gccggcgccg gtcaccagca ccctgcggcc gaccaccagc cccaccaccg aatcgtcgtc
     4081 gagcgtgatc ggctgccgcc gggtgagccg ctcgacgctg aaaaggtcgt cgaggccgtc
     4141 cgtcggcacc tccacccgcc gcgggatctg cacgacgccg agccccacgc cgctcgcctg
     4201 ctccaggagt cggcgccgct gacgctggtc gagcgcccga ctggcgatca ggaactgcgg
     4261 cgacgtgccc tgccgaccaa gccgggcgac gaccgccggc agatcggcgg aggtgccgag
     4321 gatcggcacg ccatgcagat tgcggccatg gaagtgaccc gtgtcgtcga tcacgccgac
     4381 aggccggaac ggcgctcggc tgtccgacag aagggaatgg aggtaggtcg cggcgtcgcg
     4441 gtccgcaccc cagatcagca ctgcggacgg tgtcgcgagc cggtcgaaag caccgagccg
     4501 caggccccgc cgacgggccg cctgataggc cagccgcgcg ccgcacagca gcacgacgag
     4561 caccagcgcc tggatgatcg gcaccgagcg gggcacggcc gcggcccgat cgatgacgaa
     4621 catgacgatc aggaagagca gcgaggcgaa ggccaccgcg cgcgcgagct cgacgaactc
     4681 ccgccaggcg gcgaaccgcc agatctgggt atgggtacgg ccgaggagaa atgcgatccc
     4741 ggcggtcagc atcgccactg ccgcggcaat gccaaggtcg gtgagccccc aggcggcgag
     4801 gctctccgcc ccgaggcgca ggaacatcgc gaccagaaag gcgacgccgg ccagtgccac
     4861 gtcgtgcgcc aggctgacca gcggcttgga gcgcgcgcgc gccaagcctc cggcaccacg
     4921 cagcgcgccg ccgaccgatg cccggagagg tgatctggct agcatcgacc gaaccgaccc
     4981 acttccttac ccagctccta aatcatctgt taacaacagc agaatgagtt tgcactaaac
     5041 gaggcatgtc gaaaattctt acatgattaa tacattattc cgactctggc attccatgga
     5101 gctcgccaca tgttgttaac agttcgttca tacacaatgg cgtattcaaa cgggcagtga
     5161 cttggttggc catgagcact atggtgcgtc gggattcgca gaggaattga ccaagcgggg
     5221 attggcccga caagagcgga gcccctccct gtccagcagc gcgtgccagt ggtcgggccg
     5281 ccaagctcat c
//
LOCUS       DAOTFI010000294         5162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_19077_length_5162_cov_7.178578, whole genome shotgun sequence.
ACCESSION   DAOTFI010000294 DAOTFI010000000
VERSION     DAOTFI010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19077_length_5162_cov_7.178578"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5558_21390"
     CDS             complement(<1..271)
                     /locus_tag="P5558_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26938.1"
                     /translation="MDVGRKSRRPLGPDAVVGLGVSEAPERCCEWPGCAERGDFPAPR
                     SPQALRAFLWLCLEHVRDYNRSWNYFAGMNEDEIEAHRRADFTWHR"
     gene            complement(441..884)
                     /locus_tag="P5558_21395"
     CDS             complement(441..884)
                     /locus_tag="P5558_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY26939.1"
                     /translation="MMATIKAKPVKQTGPGRSRAQDVDRHVGARMRERRIMLGLTQQQ
                     MAELIGVTYQQAHKYEKGINRIAAGRLYTIAHALGVDVSYFFEGMDNDRDFKPTPQQR
                     MLLDLARNFISLPSRKHQEAICSLARALASANDLTEFGLPPIDVK"
     gene            1198..1485
                     /locus_tag="P5558_21400"
     CDS             1198..1485
                     /locus_tag="P5558_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRY26940.1"
                     /translation="MLGIVGTELGRRLRDGLAPRHLVIRDESADHVGHAGHRPEGETH
                     FHVEIVSDGFTGRRTLDRQRMVYAAVGDLMRDRIHALTIRALAPDEAGRLG"
     gene            complement(1498..1950)
                     /locus_tag="P5558_21405"
     CDS             complement(1498..1950)
                     /locus_tag="P5558_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY26941.1"
                     /translation="MQRRIIPGVIDGQQALCTLPPEATAAEAADLMLARRVGAVMVVE
                     HGRLVGIVTERDLVFRLLACSLQPAETKLATLMTPDPETLGPDDTAVAALEKMLAGHY
                     RHLPVLRGDEVCGMVSIRDIYEAVRHMLEEELKSAESLIYGEHYGATG"
     gene            complement(2053..2583)
                     /gene="ppa"
                     /locus_tag="P5558_21410"
     CDS             complement(2053..2583)
                     /gene="ppa"
                     /locus_tag="P5558_21410"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRY26942.1"
                     /translation="MDYKKLSIGANPPHEFNVIVEVPLRADPIKYELDKESGAIFVDR
                     YLYTTMFYPCNYGFIPHTLADDGDPVDVMVIGRLPVQPGVVMRVNPIGVLKMEDEAGM
                     DEKILAKPIDQITQINRKVVSYRDVPEIDLQRIAHFFSHYKDLEPNKWVKVIGWGDAD
                     EAKQLIVQGIEREKAG"
     gene            complement(2612..3469)
                     /gene="hemF"
                     /locus_tag="P5558_21415"
     CDS             complement(2612..3469)
                     /gene="hemF"
                     /locus_tag="P5558_21415"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRY26943.1"
                     /translation="MTIEREKAAAEAWFKGLRDRLCAAFETLEDRYAGPDAAEQAPGR
                     FTFAPWQREGGGGGVMGLMKGRVFEKVGVNVSTVMGEFAPQFAGQIPGAAEDPRFWAT
                     GISLVAHPRNPHVPPAHMNTRHIRTTKSWFGGGADLNPIYAVEADTTDFHAALEGACA
                     GLDEGAYARFKQWADEYFFIPHRGEPRGVGGIFYDYLEADQEADLERHFAFTRAVGEA
                     FLDVYPRLVARHMDRAWTAEERQHQLVRRGRYVEFNLLYDRGTQFGLKTGGNPEAILM
                     SLPPVVAWP"
     gene            complement(3453..4004)
                     /locus_tag="P5558_21420"
     CDS             complement(3453..4004)
                     /locus_tag="P5558_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY26944.1"
                     /translation="MTAAAVITATTTTQGRVRLALFEPDQPGNFGAVLRLGACLGVAV
                     DVIEPCGFPLDERRIRRAAMDYATQVVWRRHAGLGEFLAVAAEEARRLVLLSTRASTA
                     YHAFAYRPRDVLVLGSESRGASPALRAACARAVRIPIRPELRSLNLATAAAIGLAEAL
                     RQLGAWPGAMAGSNEGTDVDHRA"
     gene            4271..4813
                     /gene="petA"
                     /locus_tag="P5558_21425"
     CDS             4271..4813
                     /gene="petA"
                     /locus_tag="P5558_21425"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRY26945.1"
                     /translation="MADTGNPTSDGETRRDFLGIVAWSGLGIGTVAVLWPMVYSMNPS
                     ADVLALSTTTVDLSGIEEGMAITVMWRGSPVFVRHRTTAEIEEAADVDIATLRDPEAD
                     SARVTKPEWLVVSGVCTHLGCVPLGNKPTEPRGEWGGWFCPCHGSAFDTSGRIRQGPA
                     PTNLPVPPYVFETDTSILIG"
     gene            4887..>5162
                     /locus_tag="P5558_21430"
     CDS             4887..>5162
                     /locus_tag="P5558_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRY26946.1"
                     /translation="MSTETAKPVFKNGIARWIDDRLPLLSWAQAEFVDYPAPKNLNYW
                     WNFGSLAGIVLVLQILTGLFLTMHYTPHTAMAFDSVEHIMRDVNYGWL"
BASE COUNT          771 a         1798 c         1745 g          848 t
ORIGIN      
        1 gccggtgcca ggtgaaatcc gcgcgccggt gcgcctcgat ctcgtcctcg ttcatcccgg
       61 cgaagtagtt ccagctccgg ttgtagtcgc gcacatgctc gaggcagagc cagaggaacg
      121 cgcgcagcgc ctgcggcgag cgcggtgccg ggaagtcgcc gcgctcggca cagcccggcc
      181 actcgcagca ccgctccggc gcttccgata caccgagccc gacgaccgca tccggtccga
      241 gcgggcgtcg gctcttgcgg cccacatcca tccgcgccct gccattccct cgaagctgac
      301 ccgttgccgg ccgggcgctc ggggcgagcg gccacggccg atggccagac tatggtcagt
      361 cgacggccgg tcgacaagcc gggagcggcg tggcgcggcc tgccgtcggc gtcaccgacc
      421 gccggccccc tggcggcccg tcatttgacg tcgatcggcg gcagcccgaa ctccgtcagg
      481 tcattggcgc tggcgagggc gcgggccagg ctgcagatgg cttcctgatg cttgcggctc
      541 ggcagggaga tgaaattgcg ggcgagatcg agcagcatcc gctgctgcgg cgtcggcttg
      601 aaatcccggt cgttgtccat gccctcgaag aaatagctga cgtcgacgcc gagcgcatgg
      661 gcgatggtgt agagccggcc cgccgcgatg cggttgatgc ccttctcgta cttgtgggcc
      721 tgctggtagg tgacgccgat gagctcggcc atctgctgct gggtcaggcc cagcatgatc
      781 cgccgctcgc gcatcctggc accgacatgg cggtcgacgt cctgcgctcg cgaccggccc
      841 ggtccggtct gcttcacggg cttcgccttg atcgtcgcca tcattccaaa tctccgcacg
      901 agagaagagc cggcggggcg ccggcccggg tccgacggca aaccgtcgcc cgcgatcctc
      961 cgatggttat cctgcgacgg ttaagttccg gttaattctg tcagcatagt tgtcggtcta
     1021 cgcgaaagtc acagtggaac tggcgaaaag cgtacgatgc tgcttctcct tctggttttg
     1081 aggttgcgct ttaccgaaca cgcgccggac gctacctttg gcgtctgaat tcaccctgcg
     1141 gacggccgga tgctcggggg gagcgaccgg cggcgcatca ggaccgggat agcttgcatg
     1201 ctgggcattg tcggcaccga gctcgggcgg cggctgcgag acggcctcgc cccgcggcac
     1261 ctcgtgatcc gggacgagtc cgccgatcat gtcggccatg ccggccaccg cccggaaggc
     1321 gagacgcatt tccacgtgga gatcgtgagc gacggcttca ccggccggcg aaccctcgac
     1381 cggcagcgca tggtctacgc ggcggtcggt gatctcatgc gcgaccgcat ccacgccttg
     1441 accatccgcg ccctcgcccc cgacgaggcg ggccgcctcg gctgaggcgc cggccggtca
     1501 gcctgtcgcg ccgtagtgct cgccatagat gagcgattcg gcgctcttca gctcctcctc
     1561 gagcatgtgc cggacggcct cgtagatgtc gcggatcgac accatgccgc agacctcgtc
     1621 gccgcgcagc accggcaggt ggcgatagtg gccggccagc atcttctcga gcgccgccac
     1681 ggccgtatcg tcggggccca gcgtttcggg atcgggggtc atgagcgtcg ccagcttcgt
     1741 ctccgccggc tgcagcgagc acgccaggag acgaaagacc aggtcccgct ccgtgacgat
     1801 gccgacgagg cggccgtgct cgaccaccat caccgcaccg acgcgccggg ccagcatcag
     1861 atcggccgct tcggcggcgg tcgcctcggg gggcagggtg cagagcgcct gctgcccgtc
     1921 gatcacgccc gggatgatcc gtcgctgcat cgttcgaccc ccactcgccc gcggccgggc
     1981 cggtgacccg gctgacctcg accctgcaga gatgcgggcc ggcggtcaag cacccggttc
     2041 cgggcccgcc gctcagccgg ccttctcgcg ctcgatcccc tggacgatca gctgcttcgc
     2101 ctcgtcggca tcgccccagc cgatcacctt gacccacttg tttggctcga ggtccttgta
     2161 gtgcgagaag aaatgggcga tgcgctgcag gtcgatctcc ggcacgtcgc gatagctcac
     2221 caccttgcgg ttgatctgcg tgatctggtc gatcggcttg gccaggatct tctcgtccat
     2281 gccggcctcg tcctccatct tgaggacgcc gatcgggttg acccgcatca ccacgcccgg
     2341 ctgcaccggc aggcggccga tgaccatcac gtcgaccggg tcgccgtcat cggccagcgt
     2401 atgcgggatg aacccgtaat tgcaggggta gaacatggtc gtgtagaggt agcggtcgac
     2461 gaagatcgcc ccgctctcct tgtccagctc gtacttgatc ggatcggcgc gcagcggcac
     2521 ctcgacgatg acgttgaact cgtgcggcgg gttggcgccg atcgacagct tcttgtaatc
     2581 cactgttgct tcctttgggt caggcttcgg gtcaaggcca ggcgacgacc ggtggcagcg
     2641 acatcaagat cgcctcggga ttgccgccgg tcttgaggcc gaattgcgtg ccacgatcat
     2701 agagcaggtt gaactcgaca tagcggccac gacggacgag ctggtgctgg cgctcctcag
     2761 ccgtccaggc ccggtccatg tgccgcgcca cgagcctcgg atagacgtcg aggaaggcct
     2821 cgccgaccgc gcgcgtgaag gcgaaatgcc gctcgagatc cgcctcctga tccgcctcga
     2881 gatagtcgta gaagatgccg cccacgccgc gcggctcgcc gcgatgcggg atgaagaaat
     2941 actcgtccgc ccattgcttg aaccgggcgt aggccccttc gtccagcccg gcgcaggcgc
     3001 cttccagggc ggcgtggaag tcggtcgtgt cggcctcgac cgcatagatc gggttgaggt
     3061 cggcgccgcc gccgaaccag ctcttggtgg tgcggatgtg ccgcgtgttc atgtgcgccg
     3121 gcggcacatg cggattgcgg gggtgcgcca cgagcgagat gccggtcgcc cagaagcggg
     3181 ggtcctcggc ggcccccgga atctggcccg cgaactgtgg cgcgaactcg cccatgacgg
     3241 tcgagacgtt gacccccacc ttctcgaaca cgcgcccctt catcaggccc atcacaccgc
     3301 cgccgccgcc ctcgcgctgc cagggcgcga aggtgaaccg gccgggcgcc tgctctgcgg
     3361 catcgggccc ggcatagcgg tcctcgagcg tctcgaacgc ggcgcacagc cggtcgcgaa
     3421 gccccttgaa ccaggcctcg gcggccgctt tctcacgctc gatggtcaac gtctgtccct
     3481 tcgttcgacc cggccatcgc cccgggccag gcgccgagct ggcgcagcgc ctccgccagg
     3541 ccgatcgccg cggcggtggc gaggttgagc gagcgcagct cggggcggat cgggatccgc
     3601 accgcccggg cgcacgccgc ccgaagggcg gggctcgccc cgcggctctc gctgccgagc
     3661 accagcacgt cgcgcggccg ataggcgaac gcgtgatagg ccgtcgacgc cctcgtgctc
     3721 aagaggacga gacggcgcgc ctcctccgcc gcgacggcga ggaactcccc gaggcccgca
     3781 tggcgccgcc agacgacctg cgtcgcatag tccatcgcgg cgcggcggat gcggcgctcg
     3841 tcgagcggga agccgcaggg ctcgatcacg tcgaccgcca cgccgaggca ggcgccgagg
     3901 cgcagcacgg cgccgaaatt gccgggctgg tcgggctcga agagggcgag ccgcacgcgc
     3961 ccctgcgtgg tcgtcgtcgc cgtgatcacc gccgctgccg tcatcgaagc tcaccatcga
     4021 gggcgatcga cctgccgaag ggggtccgcc gcgcattgat ttcgggcctg cgagcatggc
     4081 ggcattgcgc tccgctgcac agccgtttat gcgaccttcc gtcgcttggc ggtgtgggac
     4141 cgccctcata tcctcgccat cgcgtggccc ctcgtggtgg cggcagggtg aggcgcgcct
     4201 gtttcattcg ccagtgcgat ccgcgacggc gtggtccgtt ggtgtggatc gatcggaagg
     4261 tttggatgcc atggcagata ccggaaaccc gacctctgac ggcgagaccc ggcgtgattt
     4321 cctcgggatc gtggcctggt cggggctcgg catcggcacg gtcgcggtgc tctggcccat
     4381 ggtctacagc atgaacccct cggccgacgt gctcgccctg tcgacgacga ccgtcgacct
     4441 ctcgggcatc gaggagggca tggcgatcac cgtgatgtgg cgcggctcgc ccgtcttcgt
     4501 ccggcatcgg accaccgccg agatcgagga ggcggcggat gtcgacatcg ccacgttgcg
     4561 cgatccggaa gcggactcgg cgcgcgtgac gaagccggaa tggctcgtgg tgagcggtgt
     4621 ttgcacccat ctgggctgcg tcccgctcgg caacaagccg accgagccca ggggcgagtg
     4681 gggcggctgg ttctgcccct gccacggctc ggcgttcgac acgtccgggc gcatccgcca
     4741 ggggccggcg ccgaccaacc tgccggtgcc accctacgtc ttcgagaccg acacgagcat
     4801 tctcatcggc tgatgcccag ggcgtctccg tcgcgggcgg cgcgcaggcc gggcggtcga
     4861 gacaaccctt tcaccaggga cggaacatga gcacagaaac ggccaagccc gtcttcaaga
     4921 atgggatcgc ccgatggatc gacgaccgcc tgccgctgtt gagctgggcg caggccgagt
     4981 tcgtcgacta ccctgccccg aagaacctga actactggtg gaacttcggc tcgctcgccg
     5041 gcatcgtgct cgtcctgcag atcctcaccg gcctcttcct gacgatgcac tacacgccgc
     5101 acaccgccat ggcgttcgac agcgtcgagc acatcatgcg cgacgtgaac tacggctggc
     5161 tc
//
LOCUS       DAOTFI010000295         5160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_19092_length_5160_cov_7.022723, whole genome shotgun sequence.
ACCESSION   DAOTFI010000295 DAOTFI010000000
VERSION     DAOTFI010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19092_length_5160_cov_7.022723"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..754)
                     /gene="lepB"
                     /locus_tag="P5558_21435"
     CDS             complement(17..754)
                     /gene="lepB"
                     /locus_tag="P5558_21435"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY26947.1"
                     /translation="MTSKKSGGIKDTLKTLVYAIAIALFVRTFAYEPFNIPSGSMKPT
                     LLVGDYLFVSKFAYGYSQYSLPLGLPLFSGRIFGSLPERGDVAVFKLPSDNSTDYIKR
                     IVGLPGDRIQVINGVLQVNGEAVRLEALGPFIDDMDGRQVPIPMYAETLPGSDESHLI
                     LDLTERGRLDNTDVFVVPEGHVFAMGDNRDNSLDSRTTNVGFVPIENLIGRADLLMFS
                     VDGSARIWEPWKWPWAIRYNRLFRPIT"
     gene            complement(892..1326)
                     /gene="acpS"
                     /locus_tag="P5558_21440"
     CDS             complement(892..1326)
                     /gene="acpS"
                     /locus_tag="P5558_21440"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRY26948.1"
                     /translation="MIIGIGSDLIDIRRVEKTLERFGERFTSRCFTEVERAKADRRLR
                     RADTYAKRYAAKEACSKALGTGFSRGVFWRDIGVVNLPSGKPTLALTGGAAARLKAIT
                     PPGMGVALELTLTDEPPLAYALVILTAVPGDAPMLYPPPSVG"
     gene            complement(1382..2941)
                     /locus_tag="P5558_21445"
     CDS             complement(1382..2941)
                     /locus_tag="P5558_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRY26949.1"
                     /translation="MAQTRSATTIARIETVAFVGLEAAPVDVEVQLGSGLPSFSIVGL
                     ADKAVAESRDRVRAALAAMSLALPPKRITVNLAPGDLLKEGSHYDLPIALGLLTVMGI
                     LDSEALRGRLALGELALDGGLRPVSGVLAATLAAAERQLGLVCPLASGGEAAWLGESV
                     EVIAAPDLYSLVGHFRGKNVLPPPDPVGPTSPPVYPDLKDVKGQETAKRALEVAAAGG
                     HNLLMLGPPGAGKSMLAARLVGILPPLSAPEILELGLIRSVGGRLEAGRLPEGRPFRS
                     PHHAASMAAMVGGGRDARPGEISLAHRGVLFLDELPEFGRQVLESLRQPMEAGTVTVA
                     RANHHVTYPARFQLVAAMNPCRCGYLDDANLACARAPRCAEDYQKRISGPLFDRIDLH
                     VEVAALSVQDLTLPVPTESSAEVAARVAAARTLQAERLAGLGVATDRPPLNAELEGEL
                     LEQVCEAEPAAAELLTRAMERLRFSARGYHRILRVARTLADLEAAPVIRKVHIAEAIA
                     FRRRPPGRQAA"
     gene            3086..>3337
                     /locus_tag="P5558_21450"
     CDS             3086..>3337
                     /locus_tag="P5558_21450"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY26950.1"
                     /translation="MDYTRLGRTGLKVSVAGLGCGGSSRLGLGHGRSDREAAGIVRAA
                     FDLGVNFIDTAMAYGTETAVGMALADLPRDEVVLCTKSQV"
     assembly_gap    3338..3437
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3438..3863
                     /locus_tag="P5558_21455"
     CDS             <3438..3863
                     /locus_tag="P5558_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26951.1"
                     /translation="AFHMMHQNARATIFPQTQANGIGTLLMFAVRTIFSRPERLRQAM
                     RELAAEGKVPEALARLDEPLACLVEEHGAESLFDAAYRYVRHEPGVDVVLFGTGDRAH
                     LASNVASILRPPLPEAATRWLRELFGHLEGVGLDLPTKA"
     gene            complement(3883..4278)
                     /locus_tag="P5558_21460"
     CDS             complement(3883..4278)
                     /locus_tag="P5558_21460"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRY26952.1"
                     /translation="MKLINNFMTQGTAALVAEACLTATRAGADLEKLFEAGKASGANS
                     SVFQRMMPWVLGHNEGGMLFQLENSRKDVRYYTHLAEAVGVASFLGEAVHQTFVLAAL
                     QGEGENYVPAVARGLGQAQGVELGPKPKG"
     gene            complement(4479..>5160)
                     /locus_tag="P5558_21465"
     CDS             complement(4479..>5160)
                     /locus_tag="P5558_21465"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY26953.1"
                     /translation="EWLVQKRGYQAVKLHTWMPPIPGAPDVKMDYAACAAVREAVGPD
                     VPLMLDPNHWYSRQDALWLCQRLEELEFFWIEEMMEEASLSSYQWLTGNLRTLNILGP
                     ESMTGKLWTRAEWASKGACDMLRTGVWDVGGIGPSMKCAHIAEGFGMSCEVHGTGAAN
                     LAVAAAIPNTTYYERGLLHPFIDYDQPPAYLKRIDDEMDEDGWVHLRDEPGLGQDIDF
                     AYIEANLV"
BASE COUNT          811 a         1679 c         1818 g          752 t
ORIGIN      
        1 tttcatggcg aaacgatcag gtgatgggcc ggaagagccg gttgtagcgg atcgcccatg
       61 gccatttcca gggctcccag atgcgggccg agccgtcgac cgagaacatc aggagatcgg
      121 cccggccgat caggttctcg atcgggacga aaccgacatt ggtggtccgg ctgtcgagcg
      181 agttgtcgcg attgtcaccc atcgcgaaca catgaccttc gggaaccacg aagacgtccg
      241 tgttgtcgag ccggccgcgc tcggtgagat cgagaatgag atgcgactcg tcgctgccgg
      301 gcagggtctc ggcatacatc ggaatcggca cctggcgacc gtccatgtcg tcgatgaagg
      361 gtcccagagc ctcgagccgc accgcctcgc cattgacctg caggacgccg ttgatcacct
      421 ggatccggtc ccccggcagg ccgacgatcc gcttgatgta gtcggtgctg ttgtcggacg
      481 gcagcttgaa cacggcgaca tcgccgcgct ccggcaagga gccgaagatc cggccggaga
      541 agaggggcag cccgagcggc agggaatatt ggctgtagcc gtaggcgaac ttcgagacga
      601 acaggtagtc gccgaccagg agcgtcggct tcatcgaccc cgacgggatg ttgaacggct
      661 cgtacgcgaa ggtccgcacg aacagcgcaa tcgcgatcgc gtagacgagc gtcttcagcg
      721 tgtccttgat accgccagat ttcttcgacg tcatggcgat gcggtcacgc gaatggcggc
      781 ttgaagggga ggagacacgc ttggctatag ctcgactcgc gctgctgaca gatgggtgac
      841 cgggcgccgc agcgacaagg agagcagcgt gatcggcctg acctcgtgtc attaaccgac
      901 ggacggcggc gggtaaagca tcggcgcatc gcccggcacc gccgtcagga tcacgagggc
      961 gtaggccaaa ggcggctcgt cggtcaaggt cagctcgagg gcgacgccca tgccgggagg
     1021 cgtgatcgcc ttaagccggg cggccgcacc gccggtcagc gccagggtcg gcttgcccga
     1081 aggcaggttg acgacgccga tatcgcgcca gaagacaccc cggctgaagc ccgtgccgag
     1141 cgccttgctg caggcttcct tggcggcgta gcgcttggcg taggtgtcgg cgcggcgcag
     1201 ccgccggtcg gccttggccc gctcgacctc ggtgaagcag cggctggtga accgctcgcc
     1261 gaaccgctcg agcgtcttct cgacgcgacg gatgtcgatg aggtcgctgc cgatgccgat
     1321 gatcatggat gcctcgtccg ggggaatgcc ggtgctacga caccagcagc gccggccgcg
     1381 gtcaagccgc ctggcggccg ggcgggcggc ggcggaaggc gatggcctcg gcgatgtgga
     1441 ccttgcggat gacgggtgct gcctcgaggt cggcgagggt gcgggcgacg cgcaggatgc
     1501 ggtggtagcc gcgggccgag aagcgcagcc gctccatcgc ccgggtcaaa agctcggcgg
     1561 ccgcgggctc ggcctcgcag acctgctcga gcagctcgcc ctcgagctcg gcgttgagcg
     1621 gcggccggtc cgtcgccacg ccgagcccgg ccagccgctc ggcctgcagc gtgcgggcgg
     1681 cggcgacccg ggccgccacc tcggcggagc tctcggtcgg cactggcagg gtcaggtcct
     1741 ggacgctcag cgccgccacc tcgacatgca ggtcgatgcg atcgaagagc ggccccgaga
     1801 tgcgcttctg gtagtcctcg gcacagcgcg gtgcccgcgc acaggcgaga ttggcgtcgt
     1861 cgagatagcc gcagcggcag ggattcatgg ccgccacgag ctggaagcgg gccggatagg
     1921 tgacgtggtg gttggcccgg gccacggtga ccgtgccggc ctccatcggc tggcgcagcg
     1981 attccagcac ctggcggccg aactccggca gctcgtcgag gaaaagcacg ccgcgatggg
     2041 cgaggctgat ctcgcccggc cgggcatcac gcccgccgcc gaccattgcg gccatcgagg
     2101 cggcgtgatg cgggctgcgg aacggccggc cctcgggcag ccggccggcc tccagcctgc
     2161 cgccgacgct gcggatcagc ccgagctcga gaatctcggg agcactcaaa ggcggcagga
     2221 tgcccacgag gcgcgccgcc agcatcgatt tgccggcgcc gggcggcccc agcatcagga
     2281 ggttgtggcc gccggccgcc gctacctcga gggcgcgctt ggcggtctcc tgacccttga
     2341 cgtccttgag gtcgggatag accggggggc tggtcggtcc gaccgggtcg ggcggcggca
     2401 ggacgttctt gccgcggaaa tggccgacca gcgaatagag gtcgggggcc gcgatcacct
     2461 ccacggattc gccgagccag gccgcctcgc cgcccgaggc cagggggcag acgaggccga
     2521 gctgccgctc ggcggcggcg agtgtcgcgg ccagcacgcc cgagaccggg cgaagcccgc
     2581 catcgagggc gagctcgccc aaggcgaggc ggccacgcag cgcctcgctg tcgagaatgc
     2641 ccatgacggt cagcagcccg agggcaatag gcaggtcgta gtggctgccc tccttcagga
     2701 ggtcaccggg tgccaggttc accgtgatcc gcttgggcgg caaggcgagg ctcatggcgg
     2761 ccagcgccgc gcgcacccga tcgcggctct cggcgaccgc cttgtcggcg aggccgacga
     2821 tcgagaagga cggcaggccg ctgccgagct gcacctcgac atcgaccggt gccgcctcga
     2881 ggccgacaaa ggcgaccgtc tcgatccgag caatggtggt agctgatctg gtctgggcca
     2941 aggcgactcg cccggttgcg aatgaccgat cgagctaact ctatagttga taaaactgca
     3001 agcgatccgc gcgccccgtg tcgacagccg gacgtccggg ttctaccagt gccggcaacg
     3061 gcatcgccgg cgagggagga agacgatgga ctacacccgg ctcggccgga ccggcctgaa
     3121 ggtcagcgtc gccggactcg gctgcggcgg cagcagcagg ctcggcctcg gccatggccg
     3181 gagcgaccgc gaggccgccg ggatcgtgcg ggcggccttc gatctcgggg tcaacttcat
     3241 cgacaccgcc atggcctacg gcaccgagac ggcggtcggg atggcgctcg ccgacctgcc
     3301 gcgcgacgag gtggtgctct gcacgaagag ccaggtcnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnngcc ttccacatga tgcaccagaa cgcgcgggcg accatcttcc
     3481 cgcagacgca ggcgaacggc atcggcacac ttctgatgtt cgccgtccgc accatcttca
     3541 gccggcccga gcggttgcgc caggcgatgc gtgagctggc ggccgaaggc aaggtgccgg
     3601 aggcgctcgc ccggctcgac gagccgctgg cctgcctcgt cgaggagcat ggtgccgaga
     3661 gcctcttcga cgccgcctat cgctacgtgc gccacgagcc cggggtggac gtcgtgctct
     3721 tcggcaccgg cgaccgggcg catctcgcga gcaacgtcgc ctccatcctc cggccgccac
     3781 tgcccgaggc cgcgacgcgg tggctgcgcg agctcttcgg ccatctcgag ggcgtcggcc
     3841 tcgacctgcc gaccaaagcg tagccgcccc gggcgggaac cttcagccct ttggcttggg
     3901 gccgagctcg acgccttgtg cctggccaag cccccgtgcg acggccggca cgtagttctc
     3961 gccctcgccc tggagcgccg ccagcacgaa ggtctgatgc acggcctcgc ccaggaagct
     4021 cgccacgccg acggcctcgg cgaggtgggt gtagtagcgc acgtccttgc gcgagttctc
     4081 gagctgaaac agcatcccgc cctcgttgtg gccgaggacc cagggcatca tccgctggaa
     4141 gacgctgctg ttggcgccgc tggccttgcc ggcctcgaag agcttctcga gatcggcccc
     4201 ggccctggtg gcggtgaggc aggcctcggc gacgagtgcc gccgtgccct gggtcatgaa
     4261 gttgttgatc agcttcatcc tagggcggac gcagggagac ggtgagtcgg gtcgaaggtc
     4321 aaataggtct tgtggacccg aaaggcggac ccgaaaagcg cgaggacgat ccggcccaga
     4381 tcgagcggct gaccgcgctg atgccggaca tgaggcgggc gagcgaggaa ccgccggagc
     4441 tttgtgcccg cctcggcctg aaggcgccct gaaccgcctc agaccagatt cgcctcgata
     4501 taggcgaagt cgatgtcctg gccgaggccg ggctcgtcgc ggagatggac ccagccgtcc
     4561 tcgtccatct cgtcgtcgat ccgcttcagg taggccggcg gctggtcgta gtcgatgaac
     4621 gggtgcagca ggccgcgctc gtaataggtc gtgttgggga tggcggcggc gacggcgagg
     4681 ttggcggcgc cggtgccatg cacctcgcac gacatgccga agccttcggc gatgtgcgcg
     4741 catttcatcg acgggccgat gccgccgacg tcccagaccc ctgtgcggag catgtcgcag
     4801 gcgcccttgg acgcccactc ggcgcgggtc cagagcttgc cggtcatgct ctcggggccg
     4861 aggatgttga gtgttcggag attgcccgtc agccactggt aggagctgag cgacgcttcc
     4921 tccatcatct cctcgatcca gaagaactcc agctcctcga gccgctggca gagccacaag
     4981 gcgtcctggc ggctgtacca gtggttcggg tcgagcatca gcgggacgtc ggggccgacg
     5041 gcctcgcgca ccgcggcgca ggcggcgtag tccatcttga catccggggc acccgggatc
     5101 ggcggcatcc aggtgtgcag cttgaccgcc tggtagcccc gcttctgcac cagccactcg
//
LOCUS       DAOTFI010000296         5145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_19177_length_5145_cov_6.599804, whole genome shotgun sequence.
ACCESSION   DAOTFI010000296 DAOTFI010000000
VERSION     DAOTFI010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19177_length_5145_cov_6.599804"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..424)
                     /locus_tag="P5558_21470"
     CDS             complement(71..424)
                     /locus_tag="P5558_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26954.1"
                     /translation="MTAGAALGRAFGAVLGALALASALLPGAAPRAQGIPGDAAAGRE
                     LASRLCSGCHLIDNRQRGPALDGVPTFPEIARRLPPGEIELRLAAPSHPVMPQPPLDT
                     LGRAHILAWFATLAD"
     gene            complement(426..1595)
                     /locus_tag="P5558_21475"
     CDS             complement(426..1595)
                     /locus_tag="P5558_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRY26955.1"
                     /translation="MIQSYGGVAAMIRVSTPEIPVVGIRPHLIERRAGEVLAAFPGDV
                     LYAVKCNDHPVVLRALFDAGVRHFDTASIKEIRLVRGLFPEAACHFMHPVKSLEAIAE
                     AYFAHGVRRFVLDHPRELDKIVAATGAAEDLELFVRVAVPGDGALLTLTGKFGASVEE
                     AIALLGQCKSVARTTGVTFHVGSQCLAPQAFERAIGIVAGIIEEVGGVDHLDVGGGFP
                     AAYTGDEPAFEAFVDAILRAVGEHGLTCGLQCEPGRLLVADGASVFARVELRRGQALY
                     LNDGVYGNLAELKWVGPQFPTRALGVHGPRGGRQVSFDLFGPTCDSIDSMPGPHWLAA
                     DVADGDWIEVGMMGAYSCALRTDFNGFAAAHTVLLDDEAWYDVARLGARRLACAA"
     gene            1823..2458
                     /locus_tag="P5558_21480"
     CDS             1823..2458
                     /locus_tag="P5558_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRY26956.1"
                     /translation="MIDLYTWSTPNGRKVSIMLEECGLPYTVHEIDIGKDQQFAPDFL
                     AISPNNKIPAIVDRENGRSVFESGAILIYLAEKTGRFGGADEAERLVVLEWLMWQMGG
                     VGPMLGQAHHFLRFNPGKAPYAEERYGNEAKRLYGVADKRLATHAHLAGDNPSIADFA
                     TWPWFSRFEWQGIDLGEFPNVRRWYLDLAGRPGVQRGYDVPARGNAIPLPG"
     gene            2465..2959
                     /locus_tag="P5558_21485"
     CDS             2465..2959
                     /locus_tag="P5558_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRY26957.1"
                     /translation="MSALDRPAVQRVVLALAGAGIEGRVIELDGTARTAADAALACGV
                     PVGAIVKSLVFLVGDRPVMALVAGDRRCRMAALAGAVDLEGEVRRADAERVRTLTGFA
                     IGGVAPVGHPIPLPTVIDGSLDRFRRLFAAAGHPHCVFATDLAELARLTQGRLSDDVA
                     EAAS"
     gene            3193..3285
                     /locus_tag="P5558_21490"
     tRNA            3193..3285
                     /locus_tag="P5558_21490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3227..3229,aa:Ser,seq:gct)
     gene            complement(3377..4303)
                     /locus_tag="P5558_21495"
     CDS             complement(3377..4303)
                     /locus_tag="P5558_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26958.1"
                     /translation="MDTASATPIDVAARLAGPVRRLTVEALAGDWFMGHVRLTVEGAS
                     AQSPSQLAGRVRGALGAALRGSASAEALAGRPCPWRPPCALDLLFRSQGRITPALEIP
                     KPMVLALRREGHLLHADLGLVGFASECLEEVAAALVYAWRDRIPDVRGSRIVDRAIGS
                     TAGVAVPASARAVVLAFDSPLEIRWRDAGPASASAALWSLLSSLGNRVSGLARWQDAG
                     IQADWPELRARAATLEMTCLANDSERWQRRSFRQDRRIPMQGDRPVVRLASDLAPFLP
                     WLALGAVTHVGSHAALGMGRYRLLVEDAADTG"
     gene            4838..5041
                     /locus_tag="P5558_21500"
                     /pseudo
     CDS             4838..5041
                     /locus_tag="P5558_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          822 a         1752 c         1843 g          728 t
ORIGIN      
        1 gccagtggtc ccgctcaggg cgcccgagac acaaggctcg ggcgcccgct tctttcggcc
       61 agcgttcccg tcagtccgcc agcgtggcga accaggcgag gatatgcgcc cggcccaggg
      121 tgtcgagcgg cggctggggc atgaccgggt gggacggcgc ggcgagcctg agctcgatct
      181 ccccgggcgg cagacgtcgg gcgatctcgg ggaaggtggg caccccgtcg agcgccgggc
      241 cgcgttgccg gttgtcgatc agatggcagc cgctgcagag cctggacgcc agctcgcggc
      301 cggcagcggc atcgcccggg atgccctggg cgcgcggggc cgcgcccggc agcagggccg
      361 acgccagggc gagggcgccg agcaccgccc cgaaggctcg tccgagggcg gcgccagccg
      421 tcatctcacg ccgcgcaggc gaggcgccgg gcgccgagcc gcgccacgtc gtaccaggcc
      481 tcgtcgtcga gcaggacggt gtgggccgcc gcgaagccgt tgaagtcggt gcgcagcgcg
      541 caggaatagg cgcccatcat gccgacctcg atccagtcgc cgtcggcgac gtcggccgcc
      601 agccaatgcg gcccgggcat gctgtcgatg ctgtcgcagg tcgggccgaa gaggtcgaag
      661 ctgacctgcc gcccgccacg cggcccgtgc acgccgagcg cgcgcgtcgg gaattgcggc
      721 ccgacccact tgagctcggc gaggttgccg tagacgccgt cgttcagata gagcgcctgg
      781 ccgcggcgca gctcgacgcg cgcgaacacg gaagcgccgt cggcgacgag cagacggccg
      841 ggctcgcact gcaggccgca ggtgaggccg tgctcgccga ccgcgcgcag gatggcgtcg
      901 acgaaggctt cgaaggccgg ctcgtcaccg gtgtaggcgg ccgggaaccc gccgccgacg
      961 tcgaggtgat cgacgccgcc gacctcctcg atgatgccgg cgacgatgcc gatcgcgcgc
     1021 tcgaaggcct ggggggcgag gcattgcgag ccgacatgaa aggtgacgcc ggtcgtccgg
     1081 gccacgctct tgcactgccc gagcagcgcg atcgcttcct cgaccgaggc accgaacttg
     1141 ccggtgagcg tcaggagcgc gccatcaccc ggcacggcga cgcgcacgaa gagctccaga
     1201 tcctcggcag ccccggtggc ggccacgatc ttgtcgagct cgcgcgggtg gtcgagcacg
     1261 aagcggcgca cgccatgcgc gaaatacgcc tcggcgatcg cctccaggct cttgaccgga
     1321 tgcatgaaat ggcaggccgc ctccgggaag agaccgcgca cgaggcggat ctccttgatc
     1381 gaggcggtgt cgaagtggcg caccccggca tcgaagaggg cgcgcagcac gaccgggtgg
     1441 tcgttgcact tgaccgcgta gagcacgtcg cccgggaacg cggccagcac ctcgccggcg
     1501 cgccgctcga tcaggtgtgg ccgaatgccg accacgggaa tctctggagt ggagacgcga
     1561 atcatcgcgg ccacgccgcc gtaggactgg atcatgccga tcccccaacc cgttagagtt
     1621 aagcagacca gtatgccgcc gccgcgcgtg ccgctgtgca ttgggcaaaa ctgacgaagc
     1681 ccctatatgc ggtacggggt cctgaaagtc cagaattcga ttcaccgaat tcggcaatgc
     1741 gccccgcgga gcgcccgttg gccgccctcg gggcggcgca cccgggtcgt acgccggtgt
     1801 gtgaaaaaag aaggagaacc cattgatcga cctctatacc tggagcacgc cgaacggccg
     1861 caaggtctcc atcatgctgg aggaatgcgg cctgccctac acggtgcacg agatcgacat
     1921 cggcaaggac cagcagttcg cgcccgactt cctggcgatc agcccgaaca acaagatccc
     1981 ggcgatcgtc gaccgggaga acggccggtc ggtcttcgag agcggcgcca tcctcatcta
     2041 cctcgccgag aagaccgggc ggttcggtgg cgccgacgag gccgagcggc tggtcgtgct
     2101 cgaatggctg atgtggcaga tgggcggtgt cggcccgatg ctcggccagg cgcatcactt
     2161 cctgcgcttc aaccccggca aggcgcccta tgccgaggag cgctacggca acgaggcgaa
     2221 gcgcctctat ggcgtcgccg acaagcggct cgccacccat gcccacctcg ccggcgacaa
     2281 tcccagcatc gccgatttcg ccacctggcc ctggttctcc cgcttcgagt ggcagggcat
     2341 cgacctgggc gaattcccga atgtccgccg ctggtatctg gacctcgccg gccggccggg
     2401 cgtgcagcgc ggctatgacg tgccggcccg gggcaacgcc attccgctgc cgggctgaca
     2461 acaggtgagc gcgctcgatc gcccggccgt gcaacgggtc gttctggcgc tcgccggggc
     2521 cggcatcgag ggccgggtga tcgagctcga cggcacggcg cggaccgccg ccgatgccgc
     2581 cctggcctgc ggcgtgccgg tcggcgccat cgtcaagtcg ctggtgttcc tggtcggcga
     2641 ccggccggtc atggcgctgg tggcgggcga ccggcgatgt cgcatggcgg ccctggccgg
     2701 tgccgtcgat ctcgagggcg aggtgcggcg cgccgatgcg gagcgggtgc gcaccctgac
     2761 cggcttcgcc atcggcgggg tcgccccggt cggccacccg atccccctgc cgaccgtgat
     2821 cgacggcagc ctagaccgtt tcaggcgact gttcgcggcg gccggccacc cgcattgcgt
     2881 cttcgccacc gacctcgccg agctcgcccg cctgacgcag ggtcggctca gcgacgacgt
     2941 cgcggaggcc gcgagctgag cccgcagccg gcggcagcgc ggccgggcag ggcttcccgc
     3001 aatcgcgcca gcgagggctg acgccatgcg ccggccggag aacgcttcga tccggaccat
     3061 cgtggcgccg cgcaaggcgg gcatcggccg cttcgaggcg tgctgctgcc cggacgccga
     3121 tcggcagccc gcccgcccca tgcgccggat cattgacgct cggccccggg agcctgtaga
     3181 acccgccggt ccggagacgt ggccgagtgg ctgaaggcgg cggtttgcta aaccgttata
     3241 cagggtgaac ctgtatcggg ggttcgaatc cccccgtctc cgccagacga tcaagaatat
     3301 ttcattgttt tcaatgcgtt aacatggctc gacgaacggt ggtcccacaa ggcgacccac
     3361 gcgcggccct gacggctcaa ccggtatcgg ccgcatcctc gacgagcagg cggtagcggc
     3421 ccatgcccaa tgcggcgtga ctgccgacat gggtcaccgc acccagcgcc agccagggca
     3481 ggaacggggc gagatcgctg gcgaggcgca cgaccgggcg gtcgccctgc atgggaatgc
     3541 gccggtcctg gcggaaggag cggcgctgcc agcgctcgct gtcattggcg aggcaggtca
     3601 tttcgagggt ggccgcacgg gccctgagct cgggccagtc ggcctggatc ccggcgtcct
     3661 gccagcgggc gaggccgctc acccggttgc cgaggctgga aagcaggctc cagagggcgg
     3721 cggaggcgct ggccgggcca gcgtcgcgcc agcggatctc cagcgggctg tcgaaagcca
     3781 ggacgacggc ccgggccgag gcgggcactg ccacgcccgc ggtgctgcct atcgcccggt
     3841 cgacgatgcg gctgccccgc acgtccggga tgcggtcgcg ccaggcgtaa accagggcgg
     3901 cggcaacctc ctccaggcac tcgctggcga agccgacgag gccgagatcg gcgtggagga
     3961 ggtggccctc gcgacgcagc gccagcacca tcggcttggg gatctcgagg gccggcgtga
     4021 tccggccctg gctgcggaag aggaggtcga gggcgcaggg cgggcgccac gggcacggcc
     4081 ggccagccag cgcctcggcc gacgcgctgc cccgaagggc cgcgccgagc gcgccgcgca
     4141 cccggccggc gagctggctc ggcgactgcg ccgaggcacc ctcgaccgtc agccgaacat
     4201 gacccatgaa ccagtcgccc gccagcgcct cgaccgtcag ccgtcgcacc ggcccggcga
     4261 gccgtgcggc gacgtcgatg ggtgtcgccg atgccgtgtc catgcggcag aaaagccttg
     4321 cccggcgctt tcgcgcaagc catggtcgta cccgatttcg ccacggtttc ggcacccgcg
     4381 agggtccgca ccgtgtggag atcgacctaa cgttctgctc gacaacggaa aatcgacgtt
     4441 cttcctgcgc cgaggtgagg tgggagacgc agtcggatgg gccagcggcg acaaccttgt
     4501 ggaatcggcg ccatgaaagc gttttgtcac aaggctttgc agagtgtctc cgggagcact
     4561 gccgctgaag gaagcggatt gagacccata ggcgcgcgaa gcccgggaaa atcgccatgc
     4621 ccgggagcac tgccgctgaa ggaagcggat tgagacacag tctgctcctc ggcgacttcc
     4681 agggagtact cgggagcact gccgctgaag gaagcggatt gagacggttc ggggttgccg
     4741 tagaacttgt cccacgacta gaggggtgcg cgggtcgatc tgcttgatgg ctgcagcgtc
     4801 gaacccacct tcgatgaaga cggcccaggc aagcggtgac cagctcgttt gcgacagacg
     4861 ttttcgcatt ctcaacgttg tcgacggcgt cacccgtgag tgtttcgcgg cggccctgga
     4921 agcgtcgatc tggggtgggc gggcgggccg agaactggcg gtcctgatct tcagtcgtgg
     4981 tgagccaggc atgatcgttt ctggccacca ccccaaactc acttctaacg ctacgctcgc
     5041 ctgcggcagg acaagagggc tctctaagcc gctgtgatgc cagtgaagag ttgatcgtga
     5101 gcgtccggcc taccccacct tgctgacttg atcgtgtggt cgatg
//
LOCUS       DAOTFI010000297         5135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_19223_length_5135_cov_6.159646, whole genome shotgun sequence.
ACCESSION   DAOTFI010000297 DAOTFI010000000
VERSION     DAOTFI010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19223_length_5135_cov_6.159646"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..847
                     /locus_tag="P5558_21505"
     CDS             2..847
                     /locus_tag="P5558_21505"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRY26959.1"
                     /translation="MAAISYWDGEWRDAATPLIGPQDHAFFMASVVFDGARAFGGRTP
                     DLDRHCARVIESARGLLLEPQLSAERITELCREGVAQFAPDAELYIRPMFFAMRGFVL
                     PESGSTRFCLGINELAMPEPGEFAVCFSPYCRPARNAAPTDTKAACLYPNMQRALAEA
                     NARGFQNAITSDPNGNIAELATANLWIAKDGVAITPVCNGTFLNGITRQRVMALLRAD
                     GIAVEEATLTAADIMGADEVFTTGNYGKVLPITRVEDRRFAVGPVYRRARELYWDFAG
                     SQRAG"
     gene            978..1913
                     /locus_tag="P5558_21510"
     CDS             978..1913
                     /locus_tag="P5558_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRY26960.1"
                     /translation="MAHLGLGEATVEHGVDDARVAIVNLARGFSLETTPRQAEKVADF
                     GAVLPRGSRVFIAFIPGEQPAAILALAGRLLAEGMVPVPHIAARNLASAGAFERFARD
                     LHGVGVRQALLLAGGASHPAGSLASSLELLESGVIEGLGFEQVFVAGHPEGHADVGPG
                     ELAAALGAKNEWARRTGVPATIVTQFGFDGEAILAWAQAIGAAGNRLPIRVGVAGPAS
                     LASLLKYAKMCGVNASMTMLAKAGGRLIQLVGQSTPDGLITDLATQRQGWSELVRDLH
                     FYPFGGFERTAHWATRLSLGAITLHGRNAGFTVTN"
     gene            2036..3811
                     /locus_tag="P5558_21515"
     CDS             2036..3811
                     /locus_tag="P5558_21515"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRY26961.1"
                     /translation="MAIASRIDHARVRLRRLSGNDQLKLTVLAAAVGAAAAYGAILFR
                     ALADLTALLLFGAGEEFMAEAARSLPAWQVVMVPCLGGLAIGLACRYVLPKPLPQGVA
                     EVMEAAALRNGRMKLPVGLAAAGLSAFSIGIGGSVGREGPIVHLGATLAAQVSRWLRF
                     GPSFSRTLLGCGVAAAIATAFNAPIAGVFFALELVIGHYGLGAFAPVVIASLVGTIVV
                     RVHIGDHPAIAIDPQAIVSFWEVPGFLLLGVVAAGAAILLMGGIALVQRLHQMARVPD
                     WLRPALGGAGLGLIALVFPEVLGVGYQATETALGAMAGQAWLLALLVIAKAAATALCH
                     GSGFAGGMFSPSLTVGALVGGVFGSVASAVFPDLASPLLVYALLGMGAVAASVLGAPI
                     STIIIIFEMTTSYGITFALMGTVAVASLVTHALWGRSYFHWLLAERGVSLDDSRELGI
                     LRHRRVADVLRRDVITAPATADLEELKRRFRLSHAPIFVVGEGQALEGSIGIGDLLDA
                     AFSGDPPAGLTAAELAQPCPVVLRGSDDLATAFSRCRVHEEEHVPVLEDGDSRRILGE
                     VRFSDLLRAYNQALLEARAIEQGLR"
     gene            3886..>5135
                     /locus_tag="P5558_21520"
     CDS             3886..>5135
                     /locus_tag="P5558_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY26962.1"
                     /translation="MSNATPVIPETVEPPYLAGLNPEQRAAVTAPDGPLLVLAGAGTG
                     KTRVLTTRLAHLLVTGRARPHEVLAVTFTNKAARELSERVERLIGRSVTGLWLGTFHA
                     IGVRFLRAHAELAGLQSSFTILDTDDQLRLIKQVVQAAGLDEKRWAPRILLAIIQRWK
                     DRGHAPEDVPASEVGDFAAGQSLALYRAYQQRLSTLNACDFGDLLLHPLVVLRRHADL
                     LERYQTRFRAILVDEYQDTNVAQYLWLRLLAQRHRNITCVGDDDQSIYSWRGAEIGNI
                     LRFEQDFPGAGVVRLERNYRSTGSILGTASGLIARNQARLGKTLWTEGEAGTPVRIAG
                     LWDEAEEARYIGDEIEALQRGGESLAQIAVLVRAGFQTRAFEERFIKMGLPYRVIGGP
                     RFYERAEIRDAIAYLRLTAQPADDL"
BASE COUNT          644 a         1787 c         1895 g          809 t
ORIGIN      
        1 gatggcggcg atcagctatt gggatggcga gtggcgggat gcggcgacgc cgctgatcgg
       61 gccgcaggat catgcgttct tcatggcgag cgtcgtcttc gacggggcgc gggcgttcgg
      121 cgggcgcacg cccgacctgg accggcattg cgccagggtg atcgagtcgg ccagggggct
      181 cttgctggag ccgcagcttt cggcggagcg gatcaccgag ttgtgtcgcg agggggtggc
      241 gcagttcgcg ccggacgcgg agctctatat ccggccgatg ttctttgcca tgcgcggctt
      301 cgtgctgccg gagtcgggct cgacgcggtt ctgcctcggc atcaacgagc tggcgatgcc
      361 ggagccgggg gagttcgccg tctgcttctc gccctattgc cggccggcgc ggaacgcggc
      421 gccgaccgac accaaggccg cctgcctcta tccgaacatg cagcgggcac tggccgaggc
      481 gaatgcgcgg ggcttccaga acgccatcac ctcggacccg aacggcaata tcgcggagct
      541 ggcgacggcc aatctctgga tcgcgaagga cggcgtggcg atcacaccgg tctgcaacgg
      601 cacgttcctg aacggcatca cccggcagcg ggtgatggcg ctcctgcgcg cggacgggat
      661 cgcggtcgag gaggcgacgc tcaccgccgc cgacatcatg ggtgcggacg aggttttcac
      721 caccggcaat tacggcaagg tgctgccgat cacccgggtc gaggacaggc gcttcgcggt
      781 gggcccggtc taccggcggg cgcgcgagct ttattgggac ttcgccggca gccaacgcgc
      841 gggctgagac gcagcgacgg gcgcatcgtc gcggcggggc aagccggtgt cgcgccggca
      901 cgatcgcccc gggccgtgtg cttctacatc ctcggccatc gggtgaaggt cgcccgggtc
      961 actgggagcg gcgggcgatg gcgcatctcg gtcttggcga ggcgacggtg gagcacgggg
     1021 tggacgatgc gcgcgtcgcc atcgtcaacc tggcccgggg cttctcgctc gagacgaccc
     1081 ccaggcaggc cgagaaggtc gccgacttcg gcgccgtcct gccgcgcggc agccgggtct
     1141 tcatcgcctt catccccggc gagcagccgg cggcgatcct ggccctggcc gggcggctgc
     1201 tggccgaggg catggtgccg gtgccgcaca ttgccgcgcg caacctggcg tcggccggcg
     1261 cgttcgagcg cttcgcccgc gacctgcatg gagtcggcgt gcgccaggcg ctgctgctcg
     1321 ccggcggggc gagccacccg gccggcagcc tcgcgagctc gctcgagctg ctggagagtg
     1381 gcgtcatcga ggggctgggg ttcgagcagg tcttcgtcgc cgggcatccg gaaggccatg
     1441 cggatgtcgg gccgggggag ctggcggcgg cgctcggggc gaagaacgag tgggcgcggc
     1501 gcaccggtgt gccggcgacc atcgtcaccc agttcggctt cgacggcgag gcgatcctgg
     1561 cctgggcgca ggcgatcggt gcggcgggca accggctgcc gatccgggtc ggcgtggccg
     1621 gcccggcctc gctcgcgagc ctgctgaaat acgccaagat gtgcggcgtc aacgcctcga
     1681 tgaccatgct cgccaaggcc ggcgggcggc tcatccagct cgtcggccag tcgaccccgg
     1741 acggcctgat caccgatctc gcgacgcagc gccagggctg gtcggagctg gtgcgcgatc
     1801 tccacttcta cccgttcggc gggttcgagc ggacggcgca ttgggcgacg cggctgagcc
     1861 tgggcgccat caccctgcat ggccgcaacg cgggcttcac cgtcaccaac tgaccagtcg
     1921 cggctgcagc cttcgcccac gcatggggcg aacgggttgc cgtgagagcg caggccgcta
     1981 agcttcgccg gccgcgacag ggtgccgcgg cgatcgagcg gctgtctcct cgtccatggc
     2041 catcgccagc agaatcgatc atgccagggt gcggctccgg cgtctttcgg gcaacgacca
     2101 gctcaagctg acggtgctgg cggcggcggt cggcgctgcc gcggcctatg gcgccattct
     2161 cttcagggca ttggccgatc tcacggccct cctcctcttc ggggccggcg aggagttcat
     2221 ggcggaagcc gcccgcagcc tgccggcctg gcaggtcgtc atggtgccgt gcctcggcgg
     2281 gctcgcgatc gggctcgcct gccgctacgt cctgccgaag ccgctgccgc agggcgtggc
     2341 ggaggtcatg gaggcggcgg ccctgcgcaa cgggcggatg aagctgcccg tcggcctcgc
     2401 cgccgccggg ctcagcgcct tctcgatcgg catcggcggc tcggtcggcc gcgaggggcc
     2461 gatcgtccat ctgggcgcca ccctggcggc gcaggtctcg cgctggctgc gcttcggccc
     2521 gagcttctcg cgcacgctgc tcggctgcgg cgtggcggcg gcgatcgcca ccgccttcaa
     2581 cgcgcccatc gccggggtct tcttcgcgct cgagctggtg atcggccatt acggcctcgg
     2641 cgccttcgcg ccggtggtga tcgcgtcgct ggtcggcacc atcgtcgtcc gtgtccatat
     2701 cggcgaccat ccggccatcg ccatcgaccc gcaggcgatc gtgtcgttct gggaggtgcc
     2761 ggggttcctg ctgctcggcg tcgtcgccgc gggtgccgcc atcctgctca tgggcgggat
     2821 cgccctggtc cagcggctgc atcaaatggc gcgggtcccg gactggctgc ggccggcgct
     2881 cggcggggcg gggctcggcc tgatcgcgct cgtcttcccg gaggtgctcg gtgtcggcta
     2941 ccaggcgacg gagacggcgc tcggcgccat ggcggggcag gcctggctgc tggcgctcct
     3001 cgtcatcgcc aaggcggcgg cgacggcgct ctgccacggc agcggcttcg ccggcgggat
     3061 gttctcgccg agcctcacgg tgggcgccct ggtgggcggc gtcttcggca gcgtcgcctc
     3121 ggccgtcttt ccggatctgg cctccccgct cctggtctac gcgctcctcg gcatgggggc
     3181 ggtggcggcg agcgtcctgg gcgcgccgat ctcgacgatc atcatcatct tcgagatgac
     3241 caccagttac gggatcacct tcgcgctcat gggcacggtg gcggtggcct cgctggtgac
     3301 ccacgcgctc tgggggcgga gctatttcca ttggctcctg gccgagcggg gcgtgagcct
     3361 cgacgattcg cgcgagctcg gcatcctgcg gcatcgccgg gtggcggacg tgctgcggcg
     3421 cgacgtgatc acggctccgg ccaccgccga cctggaggag ctgaagcggc ggtttcgcct
     3481 cagccacgcg ccgatcttcg tggtgggcga ggggcaggcg ctcgagggct cgatcggcat
     3541 cggcgatctc ctggacgcgg ccttctcggg cgacccgccg gccgggctca ccgcggccga
     3601 gctggcccag ccgtgcccgg tggtgctgcg cggctcggat gacctggcca ccgccttcag
     3661 ccgctgccgg gtgcacgagg aggagcatgt gcccgtgctg gaggacggcg acagccggcg
     3721 cattctcggc gaggtgcgct tctcggacct gctgcgggcc tacaaccagg cgctgctcga
     3781 ggcgcgcgcc atcgagcagg gcctgcgctg accggccggt ggcgtctggt caccgggtgg
     3841 tggctgggct agcttgccgg cccggatcat tcgcctcatc ggtacatgtc gaacgcaacc
     3901 cccgtcatcc cggaaacggt cgagccgcct tatctcgcgg gcctgaatcc cgagcagcgg
     3961 gcggccgtga ccgcacccga cggcccgctc ctcgtgctcg ccggggcggg gaccggcaag
     4021 acgcgggtgc tcaccacgcg cctcgcgcat ctgctcgtca ccggccgcgc ccggccgcac
     4081 gaggtgctgg cggtgacgtt caccaacaag gccgcccgcg agctctccga gcgggtcgag
     4141 cggctgatcg ggcggagcgt caccgggctc tggctcggca cgttccacgc gatcggcgtg
     4201 cggttcctgc gggcccatgc cgagctcgcc gggctgcagt cgagcttcac catcctcgac
     4261 accgacgacc agctccggct gatcaagcag gtggtgcagg cggccgggct cgacgagaag
     4321 cgctgggcgc cgcgcatcct gctcgcgatc atccagcgct ggaaggatcg cggccatgcg
     4381 ccggaggacg tgccggcgag cgaggtcggc gacttcgccg ccggccagtc gctggcgctc
     4441 taccgggcct accagcagcg gctctcgacc ctgaacgcct gcgatttcgg cgatctgctg
     4501 ctgcatccgc tggtcgtcct gcgccgccat gccgacctgc tcgagcgcta ccagacgcgc
     4561 tttcgggcga tcctcgtcga cgagtaccag gacaccaacg tcgcccagta tctctggctc
     4621 aggctgctgg cgcagcggca ccggaacatc acctgcgtcg gcgacgacga ccagtcgatc
     4681 tattcgtggc ggggcgccga gatcggcaac atcctgcgct tcgagcagga tttcccgggt
     4741 gcgggtgtcg tccggctcga gcgcaactac cgctcgaccg gcagcattct cggcaccgcc
     4801 tccggcctca tcgcgcggaa ccaggcgcgg ctcggcaaga cgctctggac cgagggcgag
     4861 gccggcacgc cggtgcgcat cgcgggcctg tgggacgagg ccgaggaggc ccgctatatc
     4921 ggcgacgaga tcgaggcgct gcagcggggt ggcgagagcc tcgcccagat cgccgtgctg
     4981 gtccgcgccg gcttccagac ccgcgccttc gaggagcgct tcatcaagat ggggctgccc
     5041 tatcgggtga tcggcggccc gcgcttctac gagcgggccg agatccgcga tgccatcgcc
     5101 tatctcaggc tgaccgccca gccggccgac gacct
//
LOCUS       DAOTFI010000298         5059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_19618_length_5059_cov_7.387890, whole genome shotgun sequence.
ACCESSION   DAOTFI010000298 DAOTFI010000000
VERSION     DAOTFI010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19618_length_5059_cov_7.387890"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="P5558_21525"
     CDS             <1..303
                     /locus_tag="P5558_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRY26963.1"
                     /translation="IVKAIARLSKFYMHESCGQCTPCREGTGWLWRMMTRMAEGRARL
                     DEIDLLWDVTKEIEGHTICALGDAAAWPVQGLIRHFRPEIERRIHEYTAARQVAAE"
     gene            316..2373
                     /gene="nuoG"
                     /locus_tag="P5558_21530"
     CDS             316..2373
                     /gene="nuoG"
                     /locus_tag="P5558_21530"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRY26964.1"
                     /translation="MPKLTIDGQEVEVPAGSTVLQACEAAGREIPVFCFHPRLNIAGN
                     CRMCLVEMEKSPKPIASCAMPAADGMVIRTDTEQVHKARKGVLELLLINHPLDCPICD
                     QGGECDLQDLTLFYGPDHSRFKENKRPVTDKYLGPLISTHMTRCIHCTRCIRFINEVA
                     GVEELGACHRGEHMEITTWVEKALSSELSGNIIDLCPVGALNSKPYEYRARTWELRKT
                     ESIDVMDAVGSNIRIDARGSEVMRVLPRLHEDINEEWISDKTRFAYDGLKKRRLDVPF
                     AKQDGRLGQVSWRDAFAAIKERLDGVSGDRVGFVVGDLVDCETMVLVKELAAALGSPH
                     VDCRQDGAKLGGAARGGHLFNTTIAGIERADACLLIGTNPRHEAPLVNARLRKRHRRG
                     GFRVGRIGTPFDLTYPVTELGAGAQTLAELADGQHSFADVLERAERPMLILGQGPLAR
                     PDGAAALALASRLAERFGMIGEGWNGFNVLHTAAARVGGLDLGLVPAAGGKTVAEMVA
                     PGALDVVFLIGADEVDISGLADTFTVYLGTHGDRGVHAADVILPGAAYTEKHATYVNT
                     EGRPQRAKLAVFPPGEAKEDWKVLRALAAALGVEVKLDTLGQVRQRMAELAPHLQRFD
                     EIAAAPFEPFGTPGEMSAEPFASCVTDFYLTNPIARASAVMQQCSELFVQGAGREATG
                     THG"
     gene            2366..3376
                     /gene="nuoH"
                     /locus_tag="P5558_21535"
     CDS             2366..3376
                     /gene="nuoH"
                     /locus_tag="P5558_21535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRY26965.1"
                     /translation="MAEIWSGTLWPVLVIVFYILLIAVPLMLAMAYLTYAERKIIGGM
                     QLRQGPMTVGPYGLLQPLADGAKLFFKETVIPTGSNKIVFVLAPMVTFVLALIGWAVI
                     PFGYGLVLADINVGVLYLFAISSLGVYGIIMAGWASNSRYAFLGALRSSAQMVSYEIA
                     IGLIIINVLITAGSLNLSEIVLAQSGLWYAIPHFPMFMIFIVSILAETNRHPFDLPED
                     EATLVTGYNVEYSSMTFALFFLGEYMNMILMSAIAVILFLGGWLPPFAIFPFTLIPGP
                     IWFILKICLVLFVFIWARATLPRYRYDQLMRLGWKVFLPLSLAWVVLTSGFLVYFDML
                     PR"
     gene            3395..3883
                     /gene="nuoI"
                     /locus_tag="P5558_21540"
     CDS             3395..3883
                     /gene="nuoI"
                     /locus_tag="P5558_21540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRY26966.1"
                     /translation="MIALDRVARSFLWQELVVGFALTLKYLFKRKATLNYPYEKGPLS
                     PRFRGEHALRRYPNGEERCIACKLCEAVCPAQAITIESEPRADGSRRTTRYDIDMTKC
                     IYCGFCEEACPVDAIVEGPNFEFAAETRAELFYNKEKLLANGARWEREIAQNLAADAP
                     YR"
     gene            3938..4546
                     /locus_tag="P5558_21545"
     CDS             3938..4546
                     /locus_tag="P5558_21545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRY26967.1"
                     /translation="MTISLFLFYILAFVATAAAVMVVAARNPVHSVLFLILAFFNAAG
                     LFVLIGAEFLAMVLVVVYVGAVAVLFMFVVMMLDISSVRMRGGALQYLPIGLLVGIIL
                     LVELVLVGGTWTLAGSEIASPSPPLDSGVTNTEALGRLLYTDYFYAFQAAGVILLVAI
                     IGAITLTLRERGAIRRQDISMQVHRSRAEAVQLRKVKPRAGI"
     gene            4546..4854
                     /gene="nuoK"
                     /locus_tag="P5558_21550"
     CDS             4546..4854
                     /gene="nuoK"
                     /locus_tag="P5558_21550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRY26968.1"
                     /translation="MSIPLTHYLTLAAIIFTIGVFGIFLNRKNVIVMLMSIELILLAV
                     NINFVAFSVFLGDLAGQVFAMFILTVAAAEAAIGLAIVVVFFRNRGSIDVEDINLMKG
                     "
     gene            4867..>5059
                     /locus_tag="P5558_21555"
     CDS             4867..>5059
                     /locus_tag="P5558_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26969.1"
                     /translation="MLYIILVFAPLLGAIVAGLFGRIIGDRASQLVTCGLMGVSALCS
                     IIAIFSTVGAEPFKVPLFTW"
BASE COUNT          778 a         1750 c         1598 g          933 t
ORIGIN      
        1 atcgtgaagg cgatcgcccg gctctcgaag ttctacatgc acgagtcctg cggccagtgc
       61 acaccctgcc gcgagggcac cggctggctc tggcggatga tgacgcggat ggccgagggc
      121 cgcgcccggc tcgacgagat cgatctgctc tgggacgtga ccaaggagat cgagggccac
      181 accatctgcg cgctcggcga tgccgccgcc tggcccgtgc agggcctgat ccggcatttc
      241 cgcccggaga tcgagcggcg catccatgaa tataccgcgg cccgacaggt cgcggcggaa
      301 taggttggag ctggcatgcc gaagctgacg atcgatggcc aggaggtcga ggtgccagcc
      361 ggctcgaccg tgctgcaggc ctgcgaggcg gccggccgcg agattccggt gttctgcttc
      421 catccaaggc tgaacatcgc cggcaactgc cggatgtgcc tggtcgagat ggagaaatcg
      481 ccgaagccga tcgcttcctg cgccatgccg gcggccgatg gcatggtcat caggaccgac
      541 accgagcagg tccacaaggc caggaagggt gtcctggagc tgctcttgat caatcacccg
      601 ctcgactgcc cgatctgcga ccagggcggc gagtgcgacc tgcaggacct gaccctgttc
      661 tacgggccgg atcattcgcg cttcaaggag aacaagcgcc ccgtcaccga caagtatctg
      721 ggcccgctga tctcgaccca catgacgcgc tgcatccact gcacgcggtg catccgcttc
      781 atcaacgagg tggccggggt cgaggagctc ggtgcctgcc accgcggcga gcacatggag
      841 atcaccacct gggtggagaa ggccctgagc tcagagctct cgggcaacat catcgatctc
      901 tgcccggtgg gcgccctgaa ctcgaaaccc tacgagtatc gggcacgcac ctgggagctc
      961 aggaagaccg aatcgatcga cgtgatggat gcggtcggct cgaacatccg catcgacgcg
     1021 cgcggcagcg aggtcatgcg cgtcctgccg cggctgcacg aggacatcaa cgaggaatgg
     1081 atctccgaca agacccgctt cgcctatgac ggcctgaaga agcggcggct cgacgtcccg
     1141 ttcgccaagc aggatggccg cctcggccag gtctcctggc gggacgcctt cgccgcgatc
     1201 aaagaacggc tcgatggggt gagcggcgac cgggtcggct tcgtggtggg cgatctcgtc
     1261 gattgcgaga ccatggtcct cgtcaaggag ctggccgccg ccctcggctc gccgcatgtc
     1321 gactgccgcc aggatggggc gaagctcggc ggtgcggccc gtggcgggca tctcttcaac
     1381 acgacgatcg ccggcatcga acgggcagat gcctgcctcc tgatcggcac caacccgcgg
     1441 cacgaggcgc ccctggtcaa tgcccggctg cgcaagcgcc atcggcgcgg cgggttcagg
     1501 gtgggccgga tcggcacgcc tttcgatctc acctatccgg tgaccgagct cggtgccggt
     1561 gcgcagacgc tcgccgagct cgccgacggc cagcacagct tcgccgacgt gctcgagcgg
     1621 gccgaacggc ccatgctgat cctcggccag ggcccgctgg cccgcccgga tggtgctgcc
     1681 gccctcgccc tcgcttcgcg cctggccgag cgcttcggca tgatcggcga gggctggaac
     1741 ggcttcaacg tcctgcacac cgcggccgcc cgcgtcggcg gcctcgatct cgggctggtg
     1801 ccggcggctg gcggcaagac ggtcgccgag atggtcgcac ccggggcgct cgacgtcgtc
     1861 ttcctgatcg gcgcggacga ggtcgacatc tccggcctag ccgacacctt caccgtctat
     1921 ctcgggaccc acggcgatcg tggcgtgcat gccgcggacg tgatcctgcc gggggccgcc
     1981 tacaccgaga agcatgcgac ctacgtgaac accgagggcc gcccgcagcg ggccaagctc
     2041 gcggtttttc cgcccggcga agccaaggag gactggaagg tcctgcgggc cctggcggcg
     2101 gcgctgggcg tggaggtgaa gctcgacacg ctcggccagg ttcggcagcg catggccgag
     2161 ctggcgccgc atctgcagcg gttcgacgag atcgccgctg cccccttcga gccgttcggc
     2221 acaccgggcg agatgtcggc cgagcctttc gcctcctgcg tcaccgactt ctacctcacg
     2281 aacccgatcg cccgggcgtc ggcggtcatg cagcagtgca gcgagctctt cgtccagggc
     2341 gccgggcggg aggcgaccgg cacccatggc tgagatctgg agcggaaccc tctggccggt
     2401 cctcgtgatc gtcttctaca tcctgctgat cgccgtgccg ctgatgctgg ccatggccta
     2461 tctcacctat gccgagcgca agatcatcgg cggcatgcag cttcgccagg ggccgatgac
     2521 ggtcgggccc tacgggctgc tgcagccgct ggccgacggg gccaagctct tcttcaagga
     2581 gacggtcatc ccgaccggct ccaacaagat cgtcttcgtc ctggcgccga tggtcacctt
     2641 cgtgctggcg ctgatcggct gggccgtcat tcccttcggt tacggcctgg tgctcgccga
     2701 catcaatgtc ggcgtgctct acctgttcgc catcagctcg ctcggcgtct acggcatcat
     2761 catggccggc tgggcgtcga actcgcgcta cgcctttctc ggcgcgctgc gctcctccgc
     2821 ccagatggtc tcctacgaga tcgccatagg gctgatcatc atcaacgtgc tgatcaccgc
     2881 cgggtcgctg aacctctcgg agatcgtcct ggcccagagc gggctgtggt acgcgatccc
     2941 gcacttcccg atgttcatga tcttcatcgt ctcgatcctc gccgagacca atcgccaccc
     3001 cttcgatctg cccgaggacg aggcgaccct ggtcaccggc tacaatgtcg aatactcgtc
     3061 gatgaccttc gccctcttct tcctgggcga gtacatgaac atgatcctga tgagcgcgat
     3121 cgccgtcatc ctctttctag gcggctggct gccacccttc gccatctttc ccttcacgct
     3181 gattccgggg ccgatctggt tcattctgaa gatctgcctc gtgctcttcg tcttcatctg
     3241 ggcccgggcg accctgccgc gctaccgcta cgaccagctc atgcgactgg ggtggaaggt
     3301 gttcctgccg ctgtcgctcg cctgggtggt gctgacctcc ggctttctcg tctatttcga
     3361 tatgttgcct cgctgagatg gcggagacct tgccatgatc gctctcgacc gcgtcgcccg
     3421 aagctttctc tggcaggagc tggtggtcgg cttcgcgttg acgctgaagt atttgttcaa
     3481 gcgcaaggcg acgctcaact acccatacga gaaggggccg ctcagccccc gctttcgcgg
     3541 cgagcacgct ctgcggcgct atccgaacgg cgaggagcgc tgcatcgcct gcaagctctg
     3601 cgaggccgtc tgcccggccc aggcgatcac catcgagagc gagccgcgcg ccgacggcag
     3661 ccggcgcacc acgcgctacg acatcgacat gaccaaatgc atctattgcg gcttctgcga
     3721 ggaggcctgc ccggtcgacg cgatcgtcga gggaccgaac ttcgaattcg ccgcggaaac
     3781 gcgcgccgag ctcttctaca acaaggagaa gctgctggcg aacggtgcgc gctgggaacg
     3841 cgagatcgcc cagaaccttg ccgcagatgc cccctaccgc taagcccctc ccgcacacgc
     3901 agacctcggc cgccgtgccg tcggaaccgc ccagcccatg accataagcc tcttcctgtt
     3961 ctacatcctc gccttcgtgg ccactgccgc ggcggtgatg gtcgtggccg cccgcaaccc
     4021 ggtccattcg gtgctgttcc tgatcctggc cttcttcaat gccgccgggc tcttcgtgct
     4081 gatcggcgcc gagttcctgg cgatggtcct ggtggtcgtc tacgtcggag cggtcgccgt
     4141 cctcttcatg ttcgtcgtca tgatgctcga catcagctcg gtgcggatgc gcgggggggc
     4201 cctgcagtat ctgccgatcg gcctgctcgt cgggatcatc ctcctggtcg agctcgtcct
     4261 cgtcggcggg acctggaccc tcgccggcag cgaaatcgcg agcccgtcgc cgccgctcga
     4321 cagcggcgtg accaacaccg aggccctcgg gcggctgctc tacacggact acttctatgc
     4381 cttccaggcg gcgggcgtga tcctgctcgt cgccatcatc ggcgccatca cgctcacgct
     4441 gcgcgagcgg ggcgccatcc gccgccagga catctcgatg caggtccatc gctcgcgcgc
     4501 cgaggccgtg cagctgcgca aggtcaagcc gagggcgggc atctgatgtc gataccgctt
     4561 acccactatc tcaccctcgc cgccatcatc ttcacgatcg gtgtcttcgg catcttcctc
     4621 aaccggaaga acgtcatcgt catgctgatg tcgatcgagc tgatcctctt ggcggtcaac
     4681 atcaacttcg tggcgttctc ggtcttcctc ggcgacctcg ccgggcaggt cttcgccatg
     4741 ttcatcctca ctgtggccgc cgcggaagcg gccatcgggc tcgccatcgt ggtcgtgttc
     4801 ttccgcaacc gcggctcgat cgatgtcgaa gacatcaatc tgatgaaagg ctgagctgag
     4861 ccggccatgc tctacatcat tctcgtcttc gcgccgctgc tcggtgccat cgtcgccggg
     4921 ctgttcgggc gcatcatcgg cgaccgggcc agccagctcg tcacctgcgg cctgatgggc
     4981 gtgtcggccc tctgctcgat catcgccatc ttctcgaccg tcggcgccga gccgttcaag
     5041 gttccgctgt tcacctggg
//
LOCUS       DAOTFI010000299         4978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_20088_length_4978_cov_7.282348, whole genome shotgun sequence.
ACCESSION   DAOTFI010000299 DAOTFI010000000
VERSION     DAOTFI010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20088_length_4978_cov_7.282348"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..935)
                     /locus_tag="P5558_21560"
     CDS             complement(36..935)
                     /locus_tag="P5558_21560"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRY26970.1"
                     /translation="MTETFRLALRAVNLGRAVSGLDDWLGQVETRAREAVSAGARLLL
                     MPEYASEAWLGFKPAGLPPTGEIAWLAGVAEAALPGLQGIAERCGIALLPGTMPWAVA
                     GAFRNRAWLIRPDGRLLAQDKLCLTPFERDPESWTLAPGDRLRLFALDGWRCAVLVCL
                     DVEMPALACLLAPLHLDLVLVPSMTDATSGHARVFGCARARAVELMCAVACSGVVGSA
                     PGSTQNPRNVSGNAVYLPCEPTLGSTGVLLATPVTDGCHGEEPFDVVDLPLGVIRAIR
                     AEGGEVWPGAWSAAGVQIELEGS"
     gene            1086..1655
                     /locus_tag="P5558_21565"
     CDS             1086..1655
                     /locus_tag="P5558_21565"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRY26971.1"
                     /translation="MARARPDTDLPRTLDDFRLWHQRQPATWEFIEGMPRMMAPGSLR
                     HTLVKTNLAAALNVALRGRPCLVLADGAIVEVASSSLIPDIVVTCAEVDLAQPRVEEP
                     LVVVEIASPSSERDDLGHELDLYLRHPAVRHYLFVSQEERRLLHHERRADGDGHFLAT
                     IRTEGELVLDPPGITISLADIWSNVPLPA"
     gene            complement(1661..2299)
                     /locus_tag="P5558_21570"
     CDS             complement(1661..2299)
                     /locus_tag="P5558_21570"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRY26972.1"
                     /translation="MLETIDRHAASTAEVLGRDHIDPRVMAAVSRVPREQFVPEDERH
                     LAHGDHPLPIGHGQTISQPFIVAFMTDLLDLQPAHRVLEVGTGCGYQAAVLAELADSV
                     DTIEVVPELAAAARAQLARLGYANVRVHVGDGRLGLEDRAPFDRIIVTAAPERVPEAL
                     TRQLAPGGRLVIPVGAGPWQQTLLIVEKDEEGRLAERRTIGVAFVPLVAGDA"
     gene            2449..4170
                     /locus_tag="P5558_21575"
     CDS             2449..4170
                     /locus_tag="P5558_21575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY26973.1"
                     /translation="MTTRSKPGRLHPKVNEALDLMERGRLDRRSFVRVAALLGVGASA
                     AYAMAGLPAPAMAADDMPFPPDDPDAVMGGVLTVAMMVQRVDDPAAFSWTEMSNQARH
                     VIEYLAMTGPDNITRPMLAQSWEASDDLRTWTFHLRPGVKWHNGDDFVAEHVKWNVER
                     WVDPAVGSSNVGLSSIAALVDDVDTGEKNEDGTPKTTKRLRDGAIEVVDDLTIRFNTR
                     QPVLSFPEDLYNYPCAILHPSFTAPFSDNPIGTGPYTLVELAVGDRCSLNRVTEMTNG
                     EPFEYWGGKVYLDEIRYFNFDPENQLVAFAGGDVDAIYSFGHEQLAFAEALDGQILPA
                     RTAQTLACRMQVDVAPFDNVKLRQAMQLSLDRARIRELVFGEAGDVGDDYHVAPVHPE
                     WFPLPPLERDIDAAKALLAEAGYPDGIDLTIDVGNTNGVWQQSVCEAMRDQMQDAGIR
                     LGINVMPSSRFWEIWTQTPFGATSWTHRPLGTMVLSVGYRAGVPWNESHYNNPAFEAA
                     LDAAEAVLDVEERKVLMERVEQIMRDDAFVILPVWQPVYTIAKTTVHGYPPHPTNYHQ
                     FNKVWIG"
     gene            complement(4249..>4978)
                     /locus_tag="P5558_21580"
     CDS             complement(4249..>4978)
                     /locus_tag="P5558_21580"
                     /inference="COORDINATES: protein motif:HMM:NF018829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HRY26974.1"
                     /translation="YGEAAAATAAADHVAGLLWAARSELRADLVAPDEAVARAMRAGR
                     GPVVLVDAADNLGGGAPGDGTVLLAELLRQQAQGALVVLWDPEAVEAAAGVGVGGRFI
                     GAIGAKADRHHGTPVPVEGDVRFVGPKTYVRDGSYMKGTRVDMGHVAVLRVQGTTIVL
                     TSLRVPPFDATHLRCLGLDPAAQQILVVKSAAAWRGAFGDVVADAILTDTPGICSQRP
                     DRLPYSRLRRPVYPIDPAVVWTHS"
BASE COUNT          785 a         1835 c         1636 g          722 t
ORIGIN      
        1 gccgcggcgg aagcctattg ccgggcgctg ccggcctagg aaccctcgag ctcgatctgg
       61 acccccgccg ccgaccaggc accgggccag acctccccgc cctcagcacg tatggcgcga
      121 atgacgccga gcggcaggtc caccacatcg aacggctcct cgccgtgaca cccgtccgtc
      181 accggtgtgg cgagcagcac gccggtcgag ccgagcgtgg gctcgcacgg caggtagacc
      241 gcgttgcccg agacgtttct cgggttctgg gtgctgcccg gtgccgagcc gaccaccccg
      301 ctgcaggcga cggcgcacat cagctcgacc gcccgcgccc tggcacagcc gaagaccctg
      361 gcgtgccccg aggtcgcgtc ggtcatcgac ggcaccagga cgagatccag gtgcagtggc
      421 gccagcaggc aggcgagggc cggcatctcc acgtcgaggc agaccagcac ggcgcagcgc
      481 cagccatcga gcgcaaagag gcggagccgg tcgccgggcg cgagcgtcca gctttccggg
      541 tcgcgctcga aaggtgtcag gcagagcttg tcctgggcga ggagtcggcc gtcggggcgg
      601 atcagccagg cccggttgcg aaaggcacca gcgaccgccc agggcatggt gcccggcagg
      661 agcgctatgc cgcacctttc ggcgatcccc tgcaggccgg gcagggccgc ttcggcgacg
      721 cccgccagcc acgcgatctc gcctgtgggc ggcaaccccg ccggcttgaa gccgagccac
      781 gcctcgctcg catattctgg catcagcagc agccgcgccc cggccgagac ggcctcgcga
      841 gcccgcgttt cgacctgccc gagccagtcg tccaatccac tgacggcgcg tccgagattc
      901 acggcccgaa gcgcgaggcg aaaggtctcg gtcaaggcct gtcctccgct tgcagcgagc
      961 aatccatcac ccgttccctt cgccaatctg cgtcatcggg ggcctgcccc atttggcgag
     1021 atggcctcga ggtcgcatgt cgtaggcgat tgagctatca tatgacggcc cgagtgaggt
     1081 ttcgcatggc ccgagcacgc cccgacaccg atctgccccg aacgctggac gacttccgcc
     1141 tctggcacca gcgccagccc gcgacgtggg agttcatcga ggggatgccg cggatgatgg
     1201 cgcccggctc cctgcggcac acgctcgtca agacgaacct tgccgccgcc ctgaacgtcg
     1261 ccttgcgcgg ccgcccctgc ctcgtcctcg ccgatggcgc cattgtcgag gtcgccagct
     1321 cctcgcttat tccggacatc gtcgtgacct gcgccgaggt cgatctggca cagcccaggg
     1381 tcgaggagcc gctggtcgtc gtcgagatcg cctcaccctc gtcggagcgc gacgatctgg
     1441 gccacgagct cgacctctat ctccgccatc ccgccgtccg ccactatctc ttcgtcagcc
     1501 aggaggagcg ccgcctgctc catcacgagc gtcgcgccga tggcgatggg catttccttg
     1561 cgaccatccg gaccgagggc gagcttgtgc tcgacccgcc cggcatcacg atttcactcg
     1621 ccgacatctg gtcgaacgtg ccactgcccg cctgaccgcg tcaggcgtcg cccgccacca
     1681 gcgggacgaa ggcgacgccg atcgtccgcc gctcggcgag gcggccctct tcatccttct
     1741 cgacgatcag gagcgtctgc tgccaggggc cggcgccgac cggaatgacc agtcggccac
     1801 ccggggcgag ctggcgggtc aacgcctccg gcacccgctc gggcgctgcc gtcacgatga
     1861 tgcgatcgaa gggtgcccga tcctcgaggc cgagccggcc gtcgccgaca tggacccgga
     1921 cgttggcata gccgagccgg gccagctggg cccgcgctgc ggccgccagc tccggcacga
     1981 cctcgatcgt gtcgaccgaa tcggcgagct ccgccagcac ggccgcctga tagccgcagc
     2041 cggtgccgac ctcgagcacg cgatgcgccg gctgcaggtc gaggaggtcg gtcatgaacg
     2101 ccacgatgaa gggctgcgag atggtctggc catggccgat cggcagcgga tggtcgccat
     2161 gggccagatg gcgctcgtcc tccgggacga actgctccct cggcacccgg gagaccgcgg
     2221 ccatcacgcg ggggtcgatg tggtcgcggc cgagcacctc cgccgtcgag gcggcgtgac
     2281 ggtcgatcgt ctccagcatc gctttgaccg gctctttcat cgtggtggac agtttgcggc
     2341 gccaatagcc acctcgtcca gcccttgcgg tttttggctt gacctcggag gcccgagggg
     2401 ttcccctccc gccacgatca gaccccgagc agggtaggga gagaaccgat gaccacgcgc
     2461 tcgaagcccg gccgtttgca cccgaaggtg aacgaggcgc tcgatctcat ggaacggggc
     2521 cgcctcgacc gccgctcctt tgtccgcgtc gcggcactcc tcggcgtcgg cgcctcggcg
     2581 gcctacgcca tggcgggcct gccggcgccg gccatggcgg ccgacgacat gcccttcccg
     2641 ccggacgatc ccgatgccgt catgggcggt gtgctgaccg tcgccatgat ggtgcagcgc
     2701 gtggacgacc cggccgcctt cagctggacg gagatgtcca accaggcccg ccacgtcatc
     2761 gagtacctgg cgatgactgg gccggacaac atcacccggc cgatgctggc gcagagctgg
     2821 gaagcctccg acgacctcag gacctggacc ttccacctcc ggccaggcgt caagtggcac
     2881 aatggcgacg acttcgtcgc cgagcacgtc aagtggaacg tcgagcgctg ggtcgatccg
     2941 gcggtcggct cctccaatgt cggtctttcg tccatcgcgg ccctggtcga cgatgtcgac
     3001 acgggcgaga agaacgagga cggcacgccg aagaccacca agcgcctgcg cgacggggcc
     3061 atcgaggtgg tcgacgacct gaccatccgc ttcaacacca ggcagccggt gctttccttc
     3121 cccgaggacc tctacaacta cccctgtgcc atcctccatc cgtcgttcac cgcacccttc
     3181 tccgacaacc cgatcggcac cggcccctac acgctggtcg agctcgcggt cggcgaccgc
     3241 tgctcgctca accgggtcac cgagatgacc aatggcgagc ccttcgagta ctggggcggc
     3301 aaggtctatc tggacgagat ccgctacttc aacttcgacc ccgagaacca gctcgtcgcc
     3361 ttcgccggcg gcgatgtcga cgccatctat tccttcggcc acgagcagct cgccttcgcc
     3421 gaggcgctgg acggccagat tctgccggcc cgcaccgccc agacgctcgc ctgccgcatg
     3481 caggtcgatg tggcgccctt cgacaacgtc aagctgcgcc aggccatgca gctctcgctc
     3541 gaccgggcgc gcatccgcga gctggtgttc ggcgaggccg gcgacgtcgg cgacgactac
     3601 cacgtggccc cggtccatcc cgagtggttc ccgctgccgc cgcttgagcg cgacatcgac
     3661 gcggccaagg cgctcctcgc cgaggccggc taccccgacg gcatcgacct caccatcgac
     3721 gtcggcaaca ccaacggcgt ctggcagcag tcggtctgcg aggcgatgcg cgaccagatg
     3781 caggatgcag gcatccgcct cggcatcaac gtcatgccgt cctcgcgttt ttgggagatc
     3841 tggacgcaga cgccgttcgg cgccacctcc tggacccacc gcccgctcgg caccatggtc
     3901 ctctcggtcg gctatcgcgc cggggtcccc tggaacgaga gccactacaa caacccggcg
     3961 ttcgaggcgg cgctcgacgc ggccgaggcg gtgctcgacg tcgaggagcg aaaggtgctg
     4021 atggagcgtg tcgagcagat catgcgcgac gacgccttcg tcatcctgcc ggtctggcag
     4081 ccggtctaca ccatcgccaa gacgacggtg cacggctacc cgccccatcc gaccaactac
     4141 caccagttca acaaggtctg gatcggctag ggcgccgctc ccggccctgc ggcgaaccgg
     4201 cccggcggcc ggctcccgcc aacctcgccg aaatgacgat cgacggcgtc atgagtgcgt
     4261 ccagacgaca gccggatcga tgggatagac cggccggcga aggcgcgaat agggcaggcg
     4321 atccggccgc tgcgagcaga tgccgggcgt gtcggtgagg atggcgtcag ccacgacgtc
     4381 gccgaaggcg ccacgccagg cggccgcgga cttcaccacc aggatctgct gtgccgcggg
     4441 atcgaggccg aggcagcgca ggtgagtggc atcgaagggc ggaacacgca ggctggtcag
     4501 gacgatggtc gtgccctgca cacgcagcac cgcgacgtgg cccatgtcga cgcgcgtgcc
     4561 cttcatgtag ctgccgtcgc gcacataggt cttcggcccg acgaaccgga cgtcgccttc
     4621 gaccggcacg ggcgtgccgt gatgccggtc ggccttggcg ccgatggcgc cgatgaaacg
     4681 gccaccgacg cccacgccgg ccgccgcttc gacggcctcg ggatcccaga gcacgacgag
     4741 ggcaccctgg gcctgctggc gcaggagctc ggccaggagc acggtgccgt cgcccggcgc
     4801 accgccgccg agattgtcgg ccgcgtcgac cagcaccacc ggcccccgcc cggcgcgcat
     4861 cgcccgcgcc accgcctcgt caggtgccac gagatcggcc cggagctcgc tgcgcgccgc
     4921 ccacagaaga ccggcgacat gatcggcggc ggccgtcgct gccgctgcct cgccgtaa
//
LOCUS       DAOTFI010000300         4977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_20096_length_4977_cov_7.107070, whole genome shotgun sequence.
ACCESSION   DAOTFI010000300 DAOTFI010000000
VERSION     DAOTFI010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20096_length_4977_cov_7.107070"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..338)
                     /locus_tag="P5558_21585"
     tRNA            complement(263..338)
                     /locus_tag="P5558_21585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Arg,seq:acg)
     gene            complement(391..1026)
                     /locus_tag="P5558_21590"
     CDS             complement(391..1026)
                     /locus_tag="P5558_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRY26975.1"
                     /translation="MTRFWVLMAAKLVLAGLVVAGVVTYTLGLGPFATRLEAVTESQG
                     KALIGGPFSLVDQTGKRVTEADFAGRATLVYFGYTFCPDVCPTGLATMLAAYDKLTPQ
                     EQAKIVPLFITVDPERDDVAAMASYVALFHPALVGLTGTVEETDAAAAAWRVYHRKAE
                     SGAASDYLVDHSSFTYLMNGENEYVTHFGHGTTPEEMAEGLRAALAAGAAG"
     gene            complement(1142..2041)
                     /locus_tag="P5558_21595"
     CDS             complement(1142..2041)
                     /locus_tag="P5558_21595"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26976.1"
                     /translation="MSRRPGVAFAALGGNVQGALWLIFGAFFFSAMGLLVKLLGERLP
                     SIEIAFFRAMAGLVMVLPLMAQEGVGLWRTQRLGAHFGRALLGTGGMVCGFYAFTHLP
                     LAEATAISFTKPLFMVVLAALVLHETVRARRWTATAVGFLGVLLMLRPGSGAIELAVL
                     VALLGAAFGALTTIFIKRLVATERKSTILAYLSVVGMTLTGLPMLLVWVTPRGFDLVL
                     IVLMGVVGSIAQLCLMQAFKLGEASALAPFDYARLPFAAAFGWLIFGETLDWLALAGV
                     LVIILSSAYIAWREAYLTRLGRR"
     gene            complement(2038..3306)
                     /locus_tag="P5558_21600"
     CDS             complement(2038..3306)
                     /locus_tag="P5558_21600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY26977.1"
                     /translation="MSAGPAAAGQRVAVVGAGIVGVAAGLFLQEDGHEVTLVDPGEPG
                     SGTSSGNAGIISVSSIMPIVTADILTKLPAMLADPVAPLQLRWRDLPGLTPWLLRAAL
                     ATRRGSVERAMAAIHGLTSRALAAHEVLIQRAGAGELFTSNGWLKVGLDRARLEKGTA
                     AERAALDRFGVPYEVLGRDEVLDLEPALSSEVKGALWLTANREIRQPRAYVERLAALF
                     MERGGVHRRDVARGLAMADGRVSGLLTTEGTIAADSVVIAAGAFAGRLAAAAGHRLPL
                     QAERGYHLMLPTLASPLHRPVYCMDPGFVLAPMAHGLRLTSGVELASLEAPPDFSIPR
                     RLLPLARRYLPGLDERVLSEWQGFRPSLPDSLPVIGRAKRHANVYLAFGHQHVGLTLG
                     PVTGRIIADLVAGRDPGLDLTPYRPDRPFW"
     gene            complement(3303..4226)
                     /locus_tag="P5558_21605"
     CDS             complement(3303..4226)
                     /locus_tag="P5558_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRY26978.1"
                     /translation="MDHIQGMQVFLKVAQAGSLSAAAAQLDLSKSSVSKHVSALERRL
                     GVLLLNRSTRRLSLTELGMAYRDHCARILADIEETEQAIQAHTTRAKGRLKVNAPMSF
                     GILHLAPLLPGFMAANPEVEIDLVLNDRRVDLIDEGFDLAIRIGRLDDSNLIARKLAA
                     VGFVCAASDAYLARHRPPEVPEDLTRHNCLRYTYNRLPGEWRFSRDGEVLNVRISGNF
                     QANNGEALREAALAGLGIIYQPVFIVAGELRAGRLRPLLEAWSTPTIDIHAVFPEQRQ
                     MQPKLRHFVDHLAEALRRPELWPDLHCPVAA"
     gene            4394..>4977
                     /locus_tag="P5558_21610"
     CDS             4394..>4977
                     /locus_tag="P5558_21610"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRY26979.1"
                     /translation="MPNQLTTALAQCNPIVGDLDGNLALVRRLHRAAEQAGADLVIFP
                     EMVLTGYPLEDLVLKQALLDRTAALLETLALETAGGRPAIVLGAPWAGRGGRPFNGAF
                     LLAGGQVAAQRAKHALPNYGVFDEKRVFEAGPVPGPMRLDLPRGGTVRLGVMVCEDMW
                     IEDVAEGLEETGAQILVVINGSPFEVGKQERRIQL"
BASE COUNT          784 a         1725 c         1787 g          681 t
ORIGIN      
        1 taccggagaa ccgccgatgg ccgccgtcag ctgcaccacc accagggaca cgacctcaag
       61 ccaaggacga tccgcctcat tggcagcaat agggcggtcc ttctctccaa cacgatggaa
      121 gcatctcgat gagaggcgac acgacccaca cctttcggga aagccgacgt cggtcattcc
      181 acggccgcac agcatacgct tcctgatagc ttcctaaccg tcaaatctca ggaagcaaaa
      241 aatgtctaag tctctgattt tatggcgcgc tcggagagac tcgaactccc aaccttctga
      301 tccgtagtca gatgctctat ccattgagct acgagcgcgt gaggcgggat gtagcggctc
      361 ccggggggcg gttcaagcct tgttcgcggt tcagccggcc gcacccgcgg cgagggcggc
      421 gcgcaggccc tccgccatct cctcgggcgt ggtgccgtgg ccgaaatggg tgacgtactc
      481 gttctcgcca ttcatcaggt aggtgaagga cgagtgatcg accaggtagt cgctcgccgc
      541 cccgctctcg gccttgcggt ggtagacccg ccaggcggcg gccgcggcgt cggtctcctc
      601 gaccgtgccg gtgaggccca ccagggccgg gtggaagagg gcgacgtagc tcgccatcgc
      661 ggcgacgtcg tcgcgctcgg ggtcgacggt gatgaagagc ggcacgatct tcgcctgctc
      721 ctggggcgtg agcttgtcat aggccgccag catggtggcg agcccggtcg ggcagacgtc
      781 cgggcagaac gtgtagccga aatagacgag cgtggcgcgg ccggcgaaat cggcctccgt
      841 cacccgcttg ccggtctggt cgacgaggct gaaggggccg ccgatcagcg ccttgccctg
      901 gctttccgtc accgcctcga gccgtgtggc gaaggggccg aggccgagcg tgtaggtgac
      961 cacgccggcc accacgaggc cggccagcac cagcttggcc gccatgagaa cccagaaccg
     1021 cgtcatctcg aaaccgtttc ccgcaccgtc tccactgccc ggggagatgg acccgaccgg
     1081 gcggtcccgc cagtggcgat ccgcccgcct gcgccgagcc gtcgcggccc ggtgcggcgg
     1141 ctcagcgccg gccgaggcgc gtgagatacg cctcgcgcca ggcgatgtag gcgctgctga
     1201 ggatgatcac cagcacaccg gcgagcgcca gccagtccag ggtctcgccg aagatcagcc
     1261 agccgaaggc ggcggcgaac ggcaggcggg catagtcgaa gggggccagc gcgctcgcct
     1321 cgccaagctt gaaggcctgc atcaggcaga gctgcgcgat gctgccgacc acccccatga
     1381 ggacgatcag cacgaggtcg aagccacgcg gcgtgaccca gacgaggagc atcggcaggc
     1441 cggtcagggt catgccgacg acgctgagat aggccaggat ggtcgacttg cgctcggtcg
     1501 ccaccaatcg cttgatgaag atcgtcgtga gcgccccgaa ggcggcgccg agcagggcca
     1561 cgagcactgc cagctcgatc gccccggagc ccggccgcag catcaggaga acgccgagga
     1621 agcccacggc cgtggccgtc cagcggcggg cgcgaaccgt ctcgtgcagc accaatgctg
     1681 ccagcaccac catgaagagc ggcttggtga agctgatcgc ggtggcctcg gcgagcggca
     1741 ggtgggtgaa ggcgtagaag ccgcagacca tgccgcccgt gccgagcagg gcacggccga
     1801 aatgggcgcc gaggcgctgg gtgcgccaga gccccacacc ctcctgcgcc atcagcggca
     1861 gcaccatcac gagccccgcc atggcgcgga agaaggcgat ctcgatgctc ggcaggcgct
     1921 cgcccaggag cttcacgagg agtcccatgg ccgagaagaa gaaggcgccg aagatcagcc
     1981 agagcgcgcc ctgcacattg ccgccgagcg cggcgaaggc gacccccggg cgccggctca
     2041 ccagaacggc cggtcgggcc ggtagggggt gaggtcgagg cccgggtcgc ggccggcgac
     2101 gaggtcggcg atgatccgcc cggtgaccgg cccgagcgtc agcccgacat gctggtggcc
     2161 gaaggcgaga tagacattgg cgtggcgctt cgcccgcccg atcaccggca ggctgtccgg
     2221 caaggacggg cggaagccct gccactcgct cagcacccgc tcgtcgagac ccggcagata
     2281 gcggcgggcg agcggcagga gacgccgcgg gatcgagaag tccggcggtg cctcgaggct
     2341 cgccagctcg acgccgctgg tgagcctgag cccgtgcgcc atgggggcga ggacgaagcc
     2401 cgggtccatg cagtagaccg gccggtgcag gggcgacgcc agcgtcggca gcatgaggtg
     2461 gtagccccgc tcggcctgca gcggcaggcg gtggccggcc gcggcggcga gccggccggc
     2521 gaaggcgccg gcggcgatca ccacgctgtc ggccgcgatc gtgccctcgg tggtgagcag
     2581 cccgctgacc cgcccgtcgg ccatggcgag gccccgggcg acgtcgcgcc gatgcacgcc
     2641 gccgcgctcc atgaagagcg cggccagccg ctcgacatag gcccggggct ggcggatctc
     2701 gcgattggcg gtgagccaga gcgccccctt gacctcgctc gagagggccg gctcgaggtc
     2761 gagcacctcg tcgcggccca gcacttcata aggcacgccg aagcggtcga gggcggcccg
     2821 ctcggcggcc gtgcccttct cgagcctggc ccggtcgagc cccaccttga gccagccatt
     2881 ggaggtgaag agctcgcccg cgccggcccg ctggatcagc acctcgtgcg ccgccagcgc
     2941 ccggctggtc agcccgtgga tggcggccat ggcccgctcg accgagcccc tgcgggtggc
     3001 cagcgccgcc cgcagcagcc agggggtgag gccgggcagg tcccgccagc ggagctgcaa
     3061 gggcgccacc gggtcggcca gcatcgccgg cagcttcgtc aggatgtcgg cggtgacgat
     3121 cggcatgatc gaggagacgc tgatgatccc ggcattgccc gaggacgtgc cgctgcccgg
     3181 ctcgcccggg tcgaccagcg tcacctcgtg gccgtcctcc tgcaggaaga gcccggcggc
     3241 gacgccgacg atgccggcgc cgaccacggc gacccgctgg ccggccgcgg cgggcccggc
     3301 gctcatgccg ccaccgggca gtggaggtcc ggccagagct cgggccggcg cagcgcctct
     3361 gccagatggt cgacgaaatg gcgcagcttc ggctgcatct ggcgctgctc gggaaacacc
     3421 gcgtgaatgt cgatcgtcgg cgtcgaccag gcctcgagca ggggcctgag gcgtccggcg
     3481 cgcagctcgc ccgcgacgat gaagaccggc tggtagatga tgccgaggcc ggcgagcgcc
     3541 gcctcccgca gcgcctcgcc gttgttcgcc tggaagttgc cgctaattct cacgttcagc
     3601 acctcgccgt cgcggctgaa ccgccattca cccggcagcc ggttgtaggt gtagcgcagg
     3661 cagttgtgcc gggtgagatc ctcggggacc tcgggcggcc gatggcgggc gagataggcg
     3721 tcgctggcgg cgcagacgaa gcccacggcg gcgagcttcc tcgcgatcag gttgctgtcg
     3781 tcgagccggc cgatgcggat cgcgaggtcg aagccctcgt cgatcaggtc caccctgcgg
     3841 tcattgagca cgaggtcgat ctcgacctcg ggattggccg ccatgaagcc gggcaggagc
     3901 ggggcgaggt gcaggatgcc gaagctcatc ggcgcattga ccttgagccg gcctttggcc
     3961 ctcgtggtgt gcgcctggat cgcctgctcg gtctcctcga tgtcggcgag gatgcgggcg
     4021 caatggtcgc ggtaggccat gccgagctcg gtcagggaca gccgccgcgt gctgcggttc
     4081 aacagcagca cgccgagccg ccgctcgagc gcgctgacat gcttgctgac gctggatttc
     4141 gacaggtcga gctgggccgc cgcggccgac aggctgcccg cctgcgccac cttgaggaag
     4201 acctgcatgc cctggatgtg gtccacgtcc ggccctttcg tcggcgcatc gctttttctg
     4261 cagcctgccc gcgccaggct ggcagtggcc cgccggccat cctgcggcta gagttaggtc
     4321 gacgacagcg ctgccggtca agtgtctcga ccgacccatc agccgaccca tccgctatcg
     4381 aggaccgcca gccgtgccga accagctcac cacagccctc gcccagtgca acccgatcgt
     4441 cggcgacctc gacggcaacc tggcgctcgt ccgccggctg caccgggcgg cggagcaggc
     4501 cggggccgat ctcgtgatct tcccggagat ggtgctgacc ggctacccgc tcgaggacct
     4561 cgtgctcaag caggcgctgc tcgaccgcac cgccgccctg ctcgagacgc tggccctgga
     4621 gaccgccggc ggccgcccgg ccatcgtgct cggcgccccc tgggccgggc gtggcgggcg
     4681 gccgttcaac ggcgccttcc tgctcgccgg cggccaggtc gcggcccagc gggcgaagca
     4741 cgctttgccg aactacggcg tcttcgacga gaagcgcgtc ttcgaggcgg ggccggtgcc
     4801 ggggccgatg cggctcgact tgccccgggg cggcacggtc aggcttggcg tcatggtctg
     4861 cgaggacatg tggatcgagg acgtcgccga gggcctggag gagaccggag cgcagatcct
     4921 cgtcgtcatc aacggctcgc ccttcgaggt cggcaagcag gagcggcgca tccagct
//
LOCUS       DAOTFI010000301         4953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_20220_length_4953_cov_8.063904, whole genome shotgun sequence.
ACCESSION   DAOTFI010000301 DAOTFI010000000
VERSION     DAOTFI010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20220_length_4953_cov_8.063904"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="P5558_21615"
     CDS             <1..1377
                     /locus_tag="P5558_21615"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY26980.1"
                     /translation="LAAAEAALAEAESRREADATALASRGVELAELQRRLELADWSRS
                     GLQAARDGLEEQLAAAEAAGSERVAALQAELAGRETALAAAEAALAEAESRREADATA
                     LASRGVELAELQRRLELADWSRSGLQAARDGLEAELAAVEAALAETQSRREADATALA
                     SRGVELAELQRRLELADWSRSGLQAARDDLEERLAAAEARGAALEGELQATVASVPLI
                     ADEGARPIHAIIDLDEATSDRLAAQAAELAELRARTQAQADLLAEREAALAALEAERE
                     AEASRRLEQIGAQLGSSLRPVPVTRAAAEGAVEREMSLSSELLAATGGGDDVALLSGR
                     NVAFTAGLLFPSAEAELTPQGRAALNQIALELKQLVDALPDDLPWMLRIDGHTDDLPI
                     RSGRFRSNWELSAARASSVAEYLVDWGLPEDRLIAAGFADTQPLVAGTSEEARSRNRR
                     IELKLTLR"
     gene            complement(1427..2122)
                     /locus_tag="P5558_21620"
     CDS             complement(1427..2122)
                     /locus_tag="P5558_21620"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRY26981.1"
                     /translation="MLPLLPARAADELPQAEVERIVREYLLREPEVIMQAIEELQRRR
                     DLAASQQQRDQIAANRQMLVADSRDPAIGNPDGDVTLVEFFDYRCGYCRAMVDSINEL
                     VASDPDVRVVMKEFPILGPESLLAAKAALAADRQGAYAAMHFALMAEDRIDEAAIRRL
                     AAANDLDVDRLMADMESEPVRAHIQDNIRLAQSLGISGTPSFVVGETLLPGAVPLAAL
                     NEHIGAERSAAGG"
     gene            2377..4197
                     /locus_tag="P5558_21625"
     CDS             2377..4197
                     /locus_tag="P5558_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRY26982.1"
                     /translation="MTLDQGLVIAIVMASMALFLWGRWRHDMVAMAALLACVVLGLLP
                     AADAFSGFGHPAVITVACVLVLSRGLHESGAVDRLARAVLPNGAGGFTTIATLTVTAA
                     CLSAFMNNVGALALLMPLAIQRAQKMGLEPGQMLMPLAFGSILGGMTTLIGTPPNLIV
                     AGFRARLGEGGFSMFDFTPVGAAVAIAGIAFILLAGHWLVPVRARGSTEGFDIAAYLS
                     EVRVKDNGKAVGRTLRDIEHELEAADAQVLGMVRNDVRLAAPEPYRQVQAGDVLVIEA
                     NPEPLAEALSALDLEFEETRSRGPDNAGDEAEAPAASDGRSDGDGERPRLAEFAVLPT
                     SPITGRSATDLHLRRRWGINLLAVSRRGERNMRRLRNLPLRVGDVLLLQGATDTLGDF
                     ASQFNLVPLAERSLRLPSARSAATAAAIMAGAVILVTFGLLPAAVAFAGGVLAAMLTN
                     VVPPRSVYQAIDWPVIVLLAALIPVAGAMAETGAADLLAGHLVETVAQGQPVVALVLI
                     LVVTMFLSDFMNNAATAAVMCPIAIGTAAGLGVDADGFLMAVAVGASCAFLTPIGHQN
                     NTLILGPGGFRFGDYWRLGLPLEVVVTAVAVPLILLVWPL"
     gene            4333..>4953
                     /locus_tag="P5558_21630"
     CDS             4333..>4953
                     /locus_tag="P5558_21630"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY26983.1"
                     /translation="MTETSKIRIGATPAIRRRRFLQATTAIGAGIAAPAIISRRALSS
                     SGELNLYSWSDYVYPEMIESFESQTGITLNLATYGSNDEVLNRLRASGGTGFDVVMPS
                     ITYTPVWTEQQLLQPLDESQINVAGIIPSMWASSEGLGAVQGGKRYTTPFNWGTESLC
                     IDTDAMQATYGELGYGSLWTPEMVGLVTCRAHSALLGIGLYLDSTGE"
BASE COUNT          701 a         1741 c         1761 g          750 t
ORIGIN      
        1 ctggcggccg ccgaggcggc gctggctgaa gccgagagcc gccgcgaagc ggatgccacg
       61 gcccttgcct cacgcggcgt cgagctggcc gagctgcagc ggcggcttga gctggcggac
      121 tggtcgcgga gcggcctgca ggcggcgcgt gacgggctcg aggagcagct cgccgctgcc
      181 gaggcggccg ggagcgagcg ggtcgcggcg ctgcaggccg agctggccgg acgcgagacg
      241 gccctggcgg ccgccgaggc ggcgctggct gaagccgaga gccgccgcga agcggatgcc
      301 acggcccttg cctcacgcgg cgtcgagctg gccgagctgc agcggcggct tgagctggcg
      361 gactggtcgc ggagcggcct gcaggcagcg cgtgacgggc tcgaggccga gctggcggct
      421 gtcgaagcag cgttggccga gacccagagc cgccgcgaag cggatgccac ggcccttgcc
      481 tcacgcggcg tcgagctggc cgagctgcag cggcggcttg agctggcgga ctggtcgcgg
      541 agcggcctgc aggcggcacg cgacgatctg gaggagcgcc tggctgcggc cgaagcgcgc
      601 ggcgcggcgc tcgagggcga gctgcaggcg acggtggcgt cggtgccgct gatcgccgac
      661 gagggtgcgc ggccgatcca tgcgatcatc gatctcgacg aggcgaccag cgaccgtctg
      721 gcggcgcagg cggcggagct cgccgagctg cgggccagga cgcaagcgca ggcggacctg
      781 ctcgccgagc gcgaggcggc gctcgcagcg ctcgaggcgg agcgcgaggc ggaagcgagc
      841 cggcggctcg agcagatcgg cgcgcagctc ggctcgagcc tgcggcccgt tcccgtgacc
      901 cgggcggcgg ccgagggcgc cgtcgagcgc gagatgagcc tctcgtccga gctgctggcg
      961 gcgactggcg gcggcgacga cgtggcgctg ctcagcgggc gcaacgtggc cttcaccgca
     1021 gggctcctct tcccctcggc cgaagccgag ctgacgccgc aggggcgggc cgcgctgaac
     1081 cagatcgccc tcgagctcaa gcagctggtc gacgccttgc ccgacgacct gccctggatg
     1141 ctgcgcatcg acggtcacac cgacgacctg ccaatccgca gtggacggtt ccgctcgaac
     1201 tgggagctgt ccgcggcgcg ggcgagctcg gtcgccgagt atctggtcga ttggggtctg
     1261 ccggaggacc ggctgatcgc cgccggcttc gccgacaccc agcctctggt cgcgggcacg
     1321 agcgaggagg cgcgcagccg gaaccggcgc atcgagctca agctcaccct gcgctgaagg
     1381 acccctcggg cgggccgttc ggcgatggga tgggcatgcg cgagattcag ccgcccgcgg
     1441 ctgaccgttc ggcgccgatg tgctcgttga gagccgcgag cggcacggcg ccgggcagca
     1501 gcgtctcgcc gacgacgaag ctcggcgtgc cgctgatgcc gagcgattgg gcgaggcgga
     1561 tattgtcctg gatatgagcg cggacgggct cgctttccat gtcggccatg agccggtcga
     1621 catcgaggtc gttcgccgcc gcgagccggc ggatcgccgc ctcgtcgatc cggtcctcgg
     1681 ccatcagcgc gaaatgcatg gcggcatagg cgccctgccg gtcggcggcg agcgcagcct
     1741 tggcggcgag cagcgactcg gggccgagga tcgggaactc cttcatcacc acgcgcacat
     1801 cgggatcgga cgcgaccagc tcgttgatcg agtcgaccat cgcccggcaa taaccgcagc
     1861 gatagtcgaa gaactcgacc agcgtcacgt cgccatcggg attgccgatc gccgggtcgc
     1921 gcgaatcggc gaccaacatc tgccggttgg cggcgatctg gtcccgctgc tgctggctgg
     1981 cggccaggtc gcgccgccgc tgcagctcct cgatcgcctg catgatgact tccggctcgc
     2041 gcagcaggta ctcgcgcacg atccgctcca cttccgcctg cggcagctcg tccgccgcgc
     2101 gggccgggag aagcgggagc accaaagcga atgccagtgc ggcgaggccg agcgggcggg
     2161 gaaatacccc gatcatgcgt atcatagtac tggttctcca ttccgagagc gaccacaggc
     2221 cgctgcaggc gttgcacata gcggtacgat cggcgccatg ccagcctggc catcgacacg
     2281 aaccggtgag cgcttgctct ggcggtcccg ttcctcgact gtgtcgggac gggcatggag
     2341 agggcgcggg ccagtcggga tggagttgca gcgctcgtga cgctcgacca gggcctcgtc
     2401 atcgccatcg tgatggccag catggcgctc ttcctctggg gccgctggcg gcacgacatg
     2461 gtggccatgg cagcgctgct cgcctgtgtc gtcctcggcc tgctgccggc tgccgatgcg
     2521 ttctcgggct tcgggcatcc ggcggtgatc accgttgcct gcgtgctcgt cctgagccgc
     2581 ggtctgcacg aatcgggggc cgtagaccgg ctcgcccggg ccgtgctgcc gaacggcgcc
     2641 ggtggcttca ccacgatcgc gacgctgacc gtcaccgccg cctgcctctc ggccttcatg
     2701 aacaatgtgg gcgctctggc cttgctgatg ccgctcgcca tccagcgggc acagaagatg
     2761 ggtctcgagc ccggccagat gctcatgccg ctcgccttcg gctcgatcct cggcggcatg
     2821 accacgctga tcggcacgcc gcccaacctg atcgtcgccg gcttccgcgc acggctgggc
     2881 gagggtggct tctcgatgtt cgacttcaca ccggtcggtg ccgcggttgc catcgccggc
     2941 atcgccttca tcctgctggc cgggcactgg ctcgtgccgg tgcgggcgcg cggttcgacc
     3001 gagggcttcg acatcgccgc ctatctctcc gaggtcaggg tcaaggataa cggcaaggcc
     3061 gtcggccgga ccctgcgcga catcgagcat gagctcgagg cagcggatgc gcaggtgctg
     3121 ggcatggtac gcaacgacgt gcgcctcgcc gcccccgaac cttatcggca ggttcaggcg
     3181 ggcgatgtcc tggtcatcga ggccaatccc gagcccctgg ccgaggcgct gagcgccctc
     3241 gacctcgagt tcgaggagac tcgctcgcgc gggcccgaca atgccgggga cgaggccgaa
     3301 gcaccggccg cgagcgatgg acgcagcgac ggcgacgggg agcggccgcg gctcgcggag
     3361 ttcgccgtcc tgccgacctc accgatcacc ggccgctcgg ccaccgacct gcatctgcgc
     3421 cgacgctggg gcatcaatct gctcgccgtg tcacgccgcg gcgagcgcaa catgcgccgg
     3481 ctgcgcaacc tgccgctgcg ggtcggcgat gtcctgctgc tgcaaggtgc aacggatacg
     3541 ctcggtgact tcgccagcca gttcaacctg gtgcccttgg ccgagcgctc gctgcgcctg
     3601 ccgagtgccc ggagcgccgc cacggccgcg gcgatcatgg cgggtgcggt catcctcgtg
     3661 accttcggcc tgctgccggc cgcggtcgcc ttcgccggcg gcgtgctggc ggcgatgctg
     3721 accaatgtgg tgccgccgcg cagcgtctac caggcgatcg actggccggt gatcgtgctg
     3781 ctcgccgccc tcatcccggt cgccggggcc atggccgaga cgggtgctgc cgacctgctc
     3841 gccggccatc tggtcgagac cgtggcgcag ggccagccgg tggtggcgct ggtgctgatc
     3901 ctcgtggtca ccatgtttct ctcggacttc atgaacaacg cggcgaccgc ggcggtgatg
     3961 tgcccgatcg ccatcggcac ggccgcgggc ctcggtgtcg atgcggacgg cttcctcatg
     4021 gccgttgccg tgggcgcctc ctgcgccttc ctcaccccca tcggccacca gaacaacacg
     4081 ctgatcctcg gccccggcgg attccgcttc ggcgactact ggcgcctcgg cctgccgctc
     4141 gaggtcgtcg tgaccgccgt cgcggtgccg ctgatcctgc tggtctggcc gctctgacca
     4201 tgctctgata ggttgtcacg cgatcgtcac gaggtcggct caacgaccgc gctcatctgc
     4261 cagtagccag cgatcgacgc cagcgaccga cacaggtgat cggccggggc atcatcaggg
     4321 atggagacca ccatgaccga aaccagcaag atccggatcg gcgccacgcc cgccatcagg
     4381 cgccggcgct tcctgcaggc cactaccgcc atcggcgccg gcatcgcggc cccggcgatc
     4441 atcagccgac gcgccttgtc gtcctcgggc gagctcaatc tctacagctg gtccgactac
     4501 gtctacccgg agatgatcga gtccttcgag agccagaccg gcatcaccct caacctcgcc
     4561 acctacggct ccaacgacga ggtcctgaac cggctgcggg cttcgggcgg caccggcttc
     4621 gacgtcgtca tgccgtccat cacctacacc ccggtctgga ccgagcagca gctcttgcag
     4681 ccgctcgacg agagccagat aaacgtcgcc ggcatcattc cctcgatgtg ggcgagctcc
     4741 gagggcctcg gcgccgtgca gggcggcaag cgctacacca cgcccttcaa ctggggcacc
     4801 gaatcgctct gcatcgacac cgacgccatg caggccacct atggcgagct cggctacggc
     4861 tcgctctgga caccggagat ggtgggtctc gtcacctgcc gtgcacactc ggccctgctc
     4921 ggtatcggcc tctatctgga ttcgaccggt gag
//
LOCUS       DAOTFI010000302         4943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_20276_length_4943_cov_6.541939, whole genome shotgun sequence.
ACCESSION   DAOTFI010000302 DAOTFI010000000
VERSION     DAOTFI010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20276_length_4943_cov_6.541939"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="P5558_21635"
     CDS             <1..223
                     /locus_tag="P5558_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188860.1"
                     /note="with GsiBCD is involved in glutathione import; GsiA
                     contains 2 ATP-binding domains; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HRY26984.1"
                     /translation="LKELQALRGLTYLFISHDLAVVEALATDVMVMLRGRAVEQGPRR
                     RVFEQPEHDYTRTLLAAVPIPGRRHNERL"
     gene            852..1049
                     /locus_tag="P5558_21640"
     CDS             852..1049
                     /locus_tag="P5558_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26985.1"
                     /translation="MALRPLLSILLLTGFIAVSLVACADAEPGPGAPPQPPGEFQDPA
                     DAGTIGTVDDPIEGGDEDLKP"
     gene            1402..1566
                     /locus_tag="P5558_21645"
     CDS             1402..1566
                     /locus_tag="P5558_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26986.1"
                     /translation="MTIRAILLNLMALGILTVSLAACGDTWEGLKTDTGDNLEATGEA
                     VEDAGEAVKK"
     gene            1645..2877
                     /locus_tag="P5558_21650"
     CDS             1645..2877
                     /locus_tag="P5558_21650"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRY26987.1"
                     /translation="MTPAIDLDRLRADMLELARIGTLAGRPGVNRPSFSDADMAGRRF
                     FMERCRDAGLAPTMDTVGNVVARWDAGSGPAVMAGSHLDTVPDGGVFDGTLGTCAALE
                     AVRAMRAAGIHMKRPVEVVATADEEGRFGGMLGSQALAGTVDPLWLEAATDSDGVRLV
                     DAMRAQGLDPDRYAEARRDPTTLHAFLELHIEQGPVLEAAGLAIGIATGVSGVFNWTV
                     TLAGVANHSGTTPMSLRRDAFAGLASFAARLPGVIAALGSDQARLTIGKVELRPNFPH
                     TIPGHATFSIIGRDTDDAVMRRMAELCRRELGKAASSHGLALDIREESRLAPVELAAD
                     VAAMLAARSRHLGYATTTLPSGAGHDAQTMQHVTRAGLIFVPSKGGISHAPEEDTDWR
                     DVERGANVLLHGLIALAA"
     gene            2877..4304
                     /gene="cls"
                     /locus_tag="P5558_21655"
     CDS             2877..4304
                     /gene="cls"
                     /locus_tag="P5558_21655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRY26988.1"
                     /translation="MWLLLHIVVSLALIVRVILKPHREPASRLAWTLVILILPAIGII
                     GYLLLGETNIGRRRIERMNRILAGLPRVADAPAAHAAALRPAIPERWQPLFRVGHSIN
                     GFEPVGGNRARLMDDSNAAIEAMVADIDAATEHVHLLFYIWLPDTNGLKMAEAVKRAA
                     GRGVVCRVMVDDLGSRIFWRSEHWQAMGAAGVKLGRALPIGNPLLRIFNGRIDLRNHR
                     KIVVIDDAITYCGSQNCADPEFRIKARFAPWVDLVVRFEGPVARQNQHLFVADWMAYV
                     DERIDDLLRAPLRGFEQGITAQVVGSGPTVRYSAMPEMFETLMYSARRELVVTTPYFV
                     PDESMQAALCASARRGVATSIVFPARNDSRLVAAASRSNYAELLAAGVRIFEYGEGLL
                     HAKTLTLDGEVTLIGSANMDRRSFDLNYENNILVYDPVLSRAVRQRQESYIASSKPVT
                     LAEVEAWTLPQRLLNNTLAMLGPVL"
     gene            4379..>4943
                     /locus_tag="P5558_21660"
     CDS             4379..>4943
                     /locus_tag="P5558_21660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY26989.1"
                     /translation="MSTTRKVWTWTIALIVFLLLLYVLNDVLLPFVAGLVVAYLLDPL
                     ADRLEAMGTSRTWATALLTVGFLLVVLGGVVLLVPVIESQASALAQAFPQAIDSIWSS
                     AKPLIEGLRERFDIAGDEELQQALTSNACSAAGWAKNILGGLLTGGMAIVNIVSLLFI
                     TPVVSFYLVRDWDSMIETVDGWLPRDHR"
BASE COUNT          775 a         1804 c         1580 g          784 t
ORIGIN      
        1 cctgaaggag ctgcaggcgc ttcgcggcct cacctacctc ttcatcagcc acgacctcgc
       61 cgtggtcgag gcgctcgcca ccgacgtcat ggtgatgctg cgcggccgcg ccgtcgagca
      121 ggggccgcgc cgccgggtct tcgagcagcc cgagcacgac tatacgcgga ccctgctcgc
      181 cgccgtgccg atccccggcc ggcgccataa cgaacgctta tagcagcggt cccgccccgc
      241 cacgacggcc cccttccggc gcgaatccga ccgcacggcc cttgggttgg cgcatctggc
      301 gcaacatttc cgctcgggtt gtcgcgtcta ccgcgacaac aggcaccaga atgttgcgga
      361 ttccgcgaca taacgattcg tgatggcgca gattccgcga cattcccccc gaaatgttgc
      421 agattccgcg acacccgccg acgaacgaac ctggggaatc gacgagcgaa cccacggatt
      481 cgacgatcga acccggagaa tcgacgaacg aacccgacca tctgcgaacg aacccggcgt
      541 cgcccaaagg cttgcagcaa aatgattttt tggcttttcc accgaccatc cggcgggatg
      601 cggaccagcc gaacgtcaat tcgccgagcg aacccggaca accttgcgtt tcacgacaga
      661 ttaggatata gtacctatat cttccgtgcc cgtgcggatc ccgcggcact gttgccgatc
      721 gagaacggcg gacgcagttc gggttttccc ctgttgcgca catgcggcta gactgtcatg
      781 gcgatagcgt tcgtgcgggc cgatccggat cgcccgagcg ggcatcgacg acacagctcg
      841 aggacactgc cgtggcactc cgtccgttgc tttcgatcct gctgctcacc ggcttcatcg
      901 ccgtctcgct cgtggcctgc gccgatgccg agcccggccc cggcgcgccg ccccagccgc
      961 ccggcgaatt ccaggatccc gccgacgcgg gcacgatcgg cactgtcgac gacccgatcg
     1021 agggcggcga cgaggacctc aagccctagc aggctgttga agaagtcccg acggccgcga
     1081 cgggatcgat tttcgtcgct gggaaggcgc ccggcgcggg gcgatgccaa gcatcgtcac
     1141 gcgtcgagca acgcacgcag cggcgaaaag cggcccgtcc cggagggatg cggccatcgc
     1201 gacttcttca acagcctgct ggcatccgcc gatgtcgcgg cagtgaagca ttcccgccac
     1261 aacgtgccat cgcgtcattt cttgcggctt tcggtgcaac cgaaagcgcg ataaggaagt
     1321 tgggctattc gattgactgt gcgtcagtcg ggcgagcagc gatggtcgca aaacgccaat
     1381 gacacacgat gaggaaaagc catgacgatc cgtgcgattc tcctgaacct gatggccctc
     1441 ggtattctga ccgtctcgct cgcagcctgc ggcgacacct gggaaggcct gaagaccgac
     1501 accggcgaca acctcgaggc gaccggcgag gccgtcgagg atgccggcga ggccgtcaag
     1561 aagtagctgt tccttgcagc acccggtcac gccccgttgc gtggccgggt atcgcctcgg
     1621 tgaggcgcag tcgttgagcg gcggatgacc ccggcgatcg atctggaccg gctgcgggcg
     1681 gacatgctcg agctggcgcg gatcggcacc cttgccggcc gccccggcgt caaccggccg
     1741 agcttctcgg atgccgacat ggccggccgg cgcttcttca tggagcgctg ccgcgatgcc
     1801 ggcctcgccc ccaccatgga caccgtcggc aatgtcgtcg cccgctggga tgccggcagc
     1861 gggcctgcgg tcatggccgg ctcccatctc gacactgttc ccgacggcgg tgtcttcgac
     1921 ggcacgctcg gcacctgcgc cgccctcgag gcggtgcggg ccatgcgggc cgccggcatc
     1981 cacatgaaac ggcccgtcga ggtggtcgcc accgccgacg aggaaggccg cttcggcggc
     2041 atgctcggca gccaggcgct ggccggcacg gtcgatcccc tttggctcga ggcggccacg
     2101 gacagcgacg gcgtgcggct cgtcgacgcc atgcgcgccc agggcctcga tccggaccga
     2161 tatgccgagg cgcgccgcga ccccaccacg ctgcacgcct tcctcgagct gcatatcgag
     2221 cagggccccg tgctcgaggc cgccggcctc gccatcggca tcgcgacggg cgtctccggc
     2281 gtcttcaact ggaccgtgac gctcgctggc gtcgccaacc actccggcac gacgcccatg
     2341 agcctccgcc gcgacgcctt cgccggcctc gccagcttcg ccgcacggct cccgggcgtg
     2401 atcgccgcgc tcggttccga ccaggcccgg ctgaccatcg gcaaggtcga gctccggccc
     2461 aacttccccc acaccattcc gggccatgcc accttctcga tcatcggccg cgacaccgac
     2521 gatgccgtga tgcgccgcat ggccgagctc tgccgccgcg agctcggcaa ggcggcctcg
     2581 agccatggcc tcgccctcga catccgcgag gagagccggc tcgccccggt cgagctggcg
     2641 gcggatgtcg cggcgatgct ggcggcgcgg agccggcacc tcggctatgc caccaccacc
     2701 ctgccctcgg gcgccgggca cgacgcgcag accatgcagc acgtcacccg cgccggcctg
     2761 atcttcgtgc catccaaggg cggcatcagc catgcgcccg aggaggacac cgactggcgg
     2821 gatgtcgagc ggggcgccaa cgtgctgctg cacggcctga tcgccctggc cgcctgatgt
     2881 ggctcctcct ccatatcgtc gtctcgctcg ccctgatcgt ccgggtgatt ctcaagccgc
     2941 atcgcgaacc ggcctcgcgc ctcgcctgga ccctggtcat cctgatcctg ccggcgatcg
     3001 gcatcatcgg ctacctgctg ctcggcgaga ccaatatcgg ccggcgccgc atcgagcgga
     3061 tgaaccgcat cctggccggc ctgcccaggg tcgcggacgc cccggcggcg cacgcggcag
     3121 cactgcgccc ggcgattccc gagcgctggc agccgctctt ccgggtcggc cattcgatca
     3181 acggcttcga gccggtcggc ggcaaccgcg cccggctgat ggacgattcc aacgccgcca
     3241 tcgaggcgat ggtcgccgac atcgacgccg cgacagagca cgtccacctg ctcttctaca
     3301 tctggctgcc ggacaccaat ggcctgaaga tggccgaggc ggtcaagcgc gcggcagggc
     3361 gcggcgtcgt ctgccgggtc atggtcgacg acctcggctc gcgcatcttc tggcgcagcg
     3421 agcactggca ggcgatgggg gccgccggcg tgaagctcgg ccgcgccttg cccatcggca
     3481 atcccttgct gcgcatcttc aacggccgca tcgatctgcg caaccacagg aagatcgtcg
     3541 tcatcgacga cgccatcacc tattgcggca gccagaactg cgccgatccc gagttccgga
     3601 tcaaggcccg cttcgccccc tgggtggacc tggtggtccg cttcgagggg ccggtcgccc
     3661 gccagaacca gcatctcttc gtcgccgact ggatggccta tgtcgacgag cggatcgacg
     3721 acctcctgcg tgcccccttg cgcggcttcg agcagggcat caccgcccag gtggtcggca
     3781 gcgggccgac ggtgcgctac tcggccatgc cggagatgtt cgagaccctg atgtacagcg
     3841 cccgccgcga gctggtcgtc acgacaccct atttcgttcc ggacgaatcg atgcaggcgg
     3901 cgctctgcgc cagcgccagg cgcggcgtgg cgacgagcat cgtcttcccg gcccgcaacg
     3961 acagccgcct cgtcgccgcc gccagccgca gcaactacgc cgagctgctg gccgccgggg
     4021 tgcgcatctt cgagtacggc gagggcctgc tccacgccaa gacgctgacg ctggacggcg
     4081 aggtgacgct catcggctcc gccaacatgg accgccgcag cttcgatctg aactacgaga
     4141 acaacatcct ggtctacgac ccggtgctga gccgggcggt caggcagcgg caggagagct
     4201 atatcgcgag ctcgaagccc gtgaccctgg cggaggtgga ggcctggacg ctgccgcagc
     4261 gcctgctcaa caacacgctc gccatgctcg gccccgtgct ctgagtcgca cccgtcgcct
     4321 tcggtgcgag ccggtctagt tcctcgcccg catggcaggc cagcaaggaa acgcgcgctt
     4381 gagcaccacc cgcaaggtct ggacctggac gatcgccctg atcgtcttcc tcctcctgct
     4441 ctacgtcctg aacgacgtgc tcctgccctt cgtcgccggc ctcgtcgtcg cctacctgct
     4501 cgaccccctg gccgaccggc tggaggccat gggcacgtcc cggacctggg cgacagcact
     4561 cctgaccgtc ggcttcctgc tcgtcgtgct cggcggcgtg gtccttctgg tgccggtgat
     4621 cgagagccag gcctcggccc tcgcccaggc gttcccgcag gccatcgatt cgatctggag
     4681 ctcggcgaag ccgctgatcg aggggctgcg cgaacgcttc gacatcgccg gcgacgagga
     4741 gctgcagcag gccctgacct ccaatgcctg cagtgccgcc ggctgggcca agaacatcct
     4801 gggcggcctg ctgaccggcg gcatggcgat cgtcaacatc gtctcgctcc tgttcatcac
     4861 gccggtcgtc agcttctacc tggtgcgcga ctgggattcg atgatcgaga ccgtcgacgg
     4921 ctggctgccg cgcgaccacc ggg
//
LOCUS       DAOTFI010000303         4931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_20349_length_4931_cov_5.588392, whole genome shotgun sequence.
ACCESSION   DAOTFI010000303 DAOTFI010000000
VERSION     DAOTFI010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20349_length_4931_cov_5.588392"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="P5558_21665"
     CDS             complement(<1..1420)
                     /locus_tag="P5558_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26990.1"
                     /translation="MKHWFFGGSGAVQSPSEALPPLVVQSGVGGNSYSLNGPGYFTTL
                     AVPYDQPATGENRMLVAIVLYMAKAAGARAFVLPTWNGDDMVEGLAPNSASDKSGPGC
                     AIYYQANPDTGPADFVIDFGEDMLAAAVVLVSLTRAAQASPIDTTAGGSTTGALATIA
                     RTLTTGSPNCMILGGAMLQGWDGAPASLAAGLSAIAAGITNNANTSNDAAFTTGARTA
                     ATAGDYGVGATFQVADGLGDGWVAIKGAASSGGGGGVPSTPPTGVRFKPPTPLATVDV
                     LTVTGSGDQTWTGTASKDALVVMPNSEITGQWSVEVKGYRAIYLIGGKVRLRGRGTVT
                     TPDGRTGRGQNWWLWLETHKDAVNAAIYVSRLEVTNDPDDYSVGNSQFGDIFNLGGDV
                     NTTPATNTANWPDLFLDSILVNGGCYGWPQSVSGSFLTHSDLFKCEVGAFKSLNVSRL
                     AAPWGYQAFYSTPSYASGFRAHP"
     gene            complement(1417..2433)
                     /locus_tag="P5558_21670"
     CDS             complement(1417..2433)
                     /locus_tag="P5558_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26991.1"
                     /translation="MTIETKPAPVVYPGDDSTISWAIPWRVLAAGEVRVQLRTAAGDL
                     STLALGTDYTLAGVGASSASFSLVSIVPATGDSVLVEPYVSAVQTVDLADNDSFSVET
                     IETALDRLTTLAAMLEARIGRALAFSAFSSGVDPTLPTPAAGKALGWNATADGFANVE
                     AESIALADGAVTTAKLGAAAVTNDKIADATIQTAKLAASVLTSTWAANVDAAGYELQA
                     AALKASREVVAAAGFAAGVLTANFANGSIQTVTLTANATTVTLSGFVAGKWSGGLLFI
                     KQDGTGSRVATWPAAVKWPENTAPTLSTAAGAVDVVSLTSFDGGTTIAGALVAAGYTG
                     LAFS"
     gene            complement(2444..>4931)
                     /locus_tag="P5558_21675"
     CDS             complement(2444..>4931)
                     /locus_tag="P5558_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY26992.1"
                     /translation="VSRHMNQLEAQGVVARADDRLRRLGRDEAAKVQRQLALERKQAA
                     IMAIRRQRIDRHIATMKAAGRSAKDALLSLMHGLEGVATGIRRSAWATSAAYEQRYLG
                     GIMGRIARDMPHVEPMLKDAAFNERVAREMEQIGRQGGRPGLTGDADARRLAEVYAEH
                     AEMARRDANALGADIPQRQGWTPHTHDSMRLLRTTPREWARKIKAALDLGLTFGGMSD
                     AEIDEALAQTYRDITTGRDQVLGQGESIEQMLGTRNIAQGLHKRRELHFRSTDAWLAY
                     NREFGHGTVTGSVFDHLHKLARNAGAMEIFGPDPLRMLKRVADEQAQAIRNDASIDAK
                     KARKQIEALGRVESWASTRVMTGEAFIVGNTTIAQWGEGLRVWQALSKLGAVTISAIT
                     DPVVLANNLRFQGKGFAQAYGDVFRHYLKGRGKGEDRAFAFLLGEGYAGLIDRLVTPY
                     AAADSPIGTFSGAAATFFRWSGLTGFTDVGRAAGSRIMAADMGRHAATAWQSLPERYR
                     HVLRLHGIDAKRWEAVRRTAYAGQDGTPYLTPDRIARLDDNTIAGLVTDQIAALQQHV
                     TGQAFHVQRGALIERIRVELEIDFRRFFADEISYGVLTTDARQQKVTTWGNAPGTLNG
                     EIARTVMQLKGFPMGFSQRVIGRTLKSGPRGATPAQTFMKNAGHAGELVATLWVAGLF
                     ASWGKDLARGQTPKELWGEDGINWKTVAAAMVQSGGAGIYGDFLFASSNRFGRSALET
                     FTGPLVGEAGRLVDILVSAKNAEPRASQITSFALGNTPFLNLFYVRPALDFLILNSLR
                     ESVSPGFLKRQAKRAREEFGQSYILPREAF"
BASE COUNT          685 a         1716 c         1762 g          768 t
ORIGIN      
        1 tgggatgcgc ccggaaaccg ctcgcatagg agggcgtcga gtagaacgcc tggtagcccc
       61 agggcgcggc gagccgcgag acgttgaggc tcttgaaggc gccgacctcg cacttgaaaa
      121 ggtccgaatg ggtgaggaag gagcccgaga cgctctgcgg ccagccgtag cacccgccat
      181 tgaccaggat cgaatcgagg aagaggtccg gccagttggc ggtgttcgtc gccggcgtgg
      241 tgttcacgtc gccgccgagg ttgaaaatgt cgccgaactg cgagttgccg acgctgtagt
      301 cgtcgggatc gttcgtcacc tcgagccggg agacgtagat cgcggcattc accgcatcct
      361 tgtgcgtctc gagccagagc caccagtttt gcccgcgccc ggtccggccg tccggcgtgg
      421 tcacggtgcc tcgcccgcgc agccgcacct tgccgccgat caggtagatc gcccgatagc
      481 ccttcacctc gaccgaccac tggccggtta tctcgctgtt cggcatcacc acgagcgcgt
      541 ccttgctcgc ggtccccgtc caggtttgat cgcccgagcc ggtgacggta agcacgtcga
      601 cggttgcgag cggcgtcggc ggcttgaacc ggacgcctgt gggaggtgtc gacgggacac
      661 caccgccgcc gccgctcgag gccgcgccct tgatcgccac ccacccgtcg ccgagcccat
      721 cggcgacctg gaacgtggcg ccgaccccgt agtcgccggc ggtcgccgcg gtccgggcgc
      781 cggtggtgaa ggccgcgtcg ttcgaggtgt tggcgttgtt ggtgatgccg gccgcgatcg
      841 ccgagagccc ggccgccaga gacgccggcg cgccgtccca tccctggagc atggccccgc
      901 cgaggatcat gcagttcggc gagccggtgg tgagcgtccg ggcgatggtc gcgagcgcgc
      961 ccgtcgtcga cccgccggcc gtcgtgtcga tcggcgaggc ttgcgccgcc cgcgtgagcg
     1021 acaccaggac cacggcggcc gcgagcatgt cctcgccgaa gtcgatcacg aagtcggccg
     1081 gccccgtgtc cgggttggcc tgatagtaga tggcgcagcc gggcccggac ttgtcgctgg
     1141 cgctgttcgg cgccagccct tccaccatgt cgtcgccgtt ccaggtcggc aggacgaagg
     1201 cccgcgcccc ggccgccttg gccatgtaga gcacgatcgc caccagcatc cggttctcgc
     1261 cggtggccgg ctggtcatag ggcacggcga gcgtggtgaa gtagcccggc ccgttgaggc
     1321 tgtagctgtt gccgccgacg ccggactgga cgacgagcgg cggcagcgcc tcgctcggcg
     1381 actggaccgc ccccgagccg ccgaagaacc agtgcttcac gagaaggcca gcccggtata
     1441 gccggcggcg acgagcgccc cggcgatcgt ggtcccgcca tcgaagctgg tcaggctcac
     1501 cacgtcgacc gcgccggcgg ccgtgctcaa tgtcggcgcg gtgttctccg gccacttcac
     1561 cgccgccggc caggtggcga ccctggaccc cgtgccgtcc tgcttgatga agagcaggcc
     1621 gcccgaccac ttgccggcga cgaagcccga gagcgtcacg gtggttgcat tggccgtgag
     1681 cgtgaccgtc tgaatcgagc cgttggcaaa gttcgccgtg agcacgccgg cggcgaagcc
     1741 ggcggccgcg accacttccc ggctcgcctt caaggccgcc gcctggagct cgtagccggc
     1801 cgcgtcgacg ttcgcggccc aggtcgacgt cagcaccgag gccgccagct tggccgtctg
     1861 gatcgtggcg tcggcgatct tgtcgttggt gacggccgcg gcgccgagct tcgccgtcgt
     1921 cacggcacca tcggcgagcg cgatggactc ggcctcgaca ttggcgaagc cgtccgcggt
     1981 cgcgttccag ccgagcgcct tgccggctgc cggcgtcggc agggtcgggt ccacgccgga
     2041 ggagaaggcg gagaaggcga gcgcccggcc aatgcgcgcc tcgagcatgg ccgccagggt
     2101 ggtcagccga tccagcgccg tttcgatcgt ttcgaccgag aacgagtcgt tgtcggcgag
     2161 gtcgacggtt tgcaccgccg acacgtaagg ctcgaccagg accgagtcgc cggtcgccgg
     2221 cacgatcgac accagcgaga aggaggccga cgaggccccc acgccggcga gcgtgtagtc
     2281 ggtgccgagc gccagcgtcg agagatcacc ggccgcggtg cggagctgga cccggacttc
     2341 ccccgccgcc agcacgcgcc acgggatcgc ccaggagatc gtcgaatcgt cgcccggata
     2401 gaccaccggc gccggctttg tctcgatcgt catttctcgc ccctcagaac gcttcgcgcg
     2461 gcaggatgta gctctggccg aactcttccc gcgcccgctt cgcctgccgc ttcaggaagc
     2521 ccggcgagac gctctcgcgc aggctgttga gaatgaggaa gtcgagcgcc ggccggacgt
     2581 agaagaggtt gaggaacggc gtgttgccca gcgcgaagct ggtgatctgc gacgccctgg
     2641 gctcggcgtt cttggcggac accaggatgt cgaccagccg gccggcctcg cccaccagcg
     2701 ggccggtgaa ggtctccagc gccgagcggc cgaagcggtt ggagctggcg aagaggaagt
     2761 cgccatagat gccggcaccg cccgactgca ccatggcggc cgcaaccgtc ttccagttga
     2821 tcccgtcctc gccccagagc tccttcggcg tctggccgcg cgccaggtcc ttgccccagg
     2881 aggcgaagag gccggccacc cacaaggtgg cgaccagctc gccggcgtgc ccggcgttct
     2941 tcatgaaggt ctgggccggc gtggcgccgc gcggcccgct cttgagcgtc cggccgatca
     3001 cccgctgcga gaagcccatg ggaaagccct tgagctgcat gaccgtgcgc gcaatctcgc
     3061 cgttcagggt gcccggcgca tttccccagg tggtcacctt ctgctggcgg gcatcggtgg
     3121 tcagcacccc ataggagatc tcgtcggcga agaagcggcg aaagtcgatc tcgagctcga
     3181 ccctgatccg ttcgatcagc gccccccgtt gcacgtgaaa cgcctggccg gtgacgtgct
     3241 gctggagcgc ggcgatctgg tcggtcacga ggccggcgat ggtgttgtcg tcgagccggg
     3301 cgatccggtc gggggtgagg tagggggtgc cgtcctggcc ggcataggcc gtccggcgca
     3361 cggcctccca gcgcttggcg tcgatgccgt gcaggcggag cacgtggcgg tagcgctcgg
     3421 gcaggctctg ccaggcggtg gcggcgtggc ggcccatgtc ggccgccatg atcctcgagc
     3481 ccgcggcgcg gccgacatcg gtgaagccgg tgaggcccga ccagcgaaag aacgtggcgg
     3541 cggcgcccga gaacgtgccg atcggcgaat cggccgccgc gtagggcgtc accagccggt
     3601 cgatcagccc cgcgtagccc tcgcccaaaa ggaaggcgaa ggcccggtcc tcgcccttgc
     3661 cgcgaccctt gaggtagtgc cggaacacgt cgccataggc ctgggcgaag cccttgccct
     3721 ggaagcgcag gttgttggcc agcaccacgg ggtcggtgat ggcggagatc gtcaccgccc
     3781 cgagcttgga gagcgcctgc cagacgcgca ggccctcgcc ccattgggcg atggtggtgt
     3841 tgccgacgat gaaggcctcg ccggtcatca cccgggtcga ggcccagctc tcgacccggc
     3901 cgagcgcctc gatctgcttc ctcgccttct tcgcgtcgat cgaggcatcg ttgcggatcg
     3961 cctgcgcctg ctcgtcggcg acccgcttca gcatgcgcag cgggtccggg ccgaagatct
     4021 ccatggcccc cgcattgcgc gccagcttgt gcagatggtc gaagaccgag ccggtcacgg
     4081 tgccgtggcc gaactccctg ttgtaggcga gccaggcgtc ggtcgagcgg aaatgcagct
     4141 cgcgccgctt gtgcaggccc tgggcgatgt tgcgggtgcc gagcatctgc tcgatgctct
     4201 cgccctggcc cagcacctgg tcgcggccgg tggtgatgtc gcgataggtc tgcgcgagcg
     4261 cctcgtcgat ctcggcgtcg ctcatcccgc cgaaggtgag gccgaggtcg agggccgcct
     4321 tgatcttccg ggcccactcc ctgggagtgg tcctgagcag ccgcatgctg tcgtgggtgt
     4381 gcggcgtcca gccctggcgc tgggggatgt cggcgccgag cgcgttggcg tcgcggcgcg
     4441 ccatctcggc gtgctcggca tagacctcgg ccagccggcg cgcgtcggcg tcgccggtga
     4501 ggccgggccg gccgccctgc ctgccgatct gctccatctc gcgcgccacg cgctcgttga
     4561 aggcggcgtc cttgagcatg ggctcgacat gcggcatgtc gcgcgcgatc cggcccatga
     4621 tgccgcccag atagcgctgc tcgtaggccg ccgaggtggc ccaggcggag cggcggatgc
     4681 cggtcgccac gccttcgagc ccgtgcatca gcgagagcag cgcgtccttg gcgctgcggc
     4741 cggcggcctt catggtcgcg atgtgccggt cgatgcgctg ccgcctgatc gccatgatcg
     4801 ccgcctgctt gcgctcgagc gcaagctgcc gctgcacctt ggccgcctcg tcgcggccga
     4861 gccgccgcag gcggtcgtcg gcgcgcgcca ccacgccctg cgcctcgagc tggttcatgt
     4921 ggcgggagac g
//
LOCUS       DAOTFI010000304         4897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_20523_length_4897_cov_8.398596, whole genome shotgun sequence.
ACCESSION   DAOTFI010000304 DAOTFI010000000
VERSION     DAOTFI010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20523_length_4897_cov_8.398596"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="P5558_21680"
     CDS             <1..352
                     /locus_tag="P5558_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRY26993.1"
                     /translation="VEIAVAGVEDIGDAQPVANASHAGDVNLHPLILYNVNDPADALK
                     AEEAGNDILALCVEVGGCLTGEHGVGIEKRDLMHRQFNAVDLDQQLRVRAVFDAQGLL
                     NPGKVFPLDSRIAA"
     gene            349..1536
                     /locus_tag="P5558_21685"
     CDS             349..1536
                     /locus_tag="P5558_21685"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY26994.1"
                     /translation="MTLHEPASEEEAAALVAGATAPLRLQGGGTRARIGRPIQTAATL
                     TSRSLAGITLYEPAEMVIGAKSGTPLAEIEATLAERNQMLTFEPMDHRPLFASDGEPT
                     IGAVAAANISGPRRVFGGAARDSLIGVRFVNGRGQIIKNGGRVMKNVTGLDLVKLQAG
                     AYGTLGFLTEVIFKVLPRPAAATTLVYARLGDARAVALMCEAMGTPFEITGAAHLPAG
                     LGAVEARTLLRLEGFPEQLDYRGGRLAGHFSAFGSPERLDGEAGAALWREVRDVRPLV
                     EPSDHAVWRLSLPPKAGAATVAAIRRSLAGVDLRVLYDWSGGLVWLALPEADDAGAAA
                     IRAATRDAKGHATLVRGSAELRARIAVFEPEPAAVAALTQGVKTSFDPRGLLNPGLMH
                     PGG"
     gene            complement(1505..2446)
                     /locus_tag="P5558_21690"
     CDS             complement(1505..2446)
                     /locus_tag="P5558_21690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY26995.1"
                     /translation="MDETTVDRAAAIRRGYALGLVGVIIFSLSLPGTRLAVPELGAEL
                     VTAGRAALAGLLSLAYLLVGRRRLPARRHWRDLGLVIGGVVIGFPLCTALAMREVEAS
                     HGGVVLSLMPLMTAGTGALFAGERPSLRFWLAALAGTAVVLAFMLYRAGGFSQADLYL
                     ALAGLGASIGYAGGARMTAHMPALEAISWSLALALPVSLPLMLFLWPADPAAASLVAW
                     GALGFVAAFPQFIGFFFWYGGLAAGGIARVGQMQLLQVYFTLGFSALLLGERVDLVTW
                     VAAVLTVFCVWLGRKAPVRSRAEGAAHLSHPDASGRD"
     gene            2536..3825
                     /gene="glcF"
                     /locus_tag="P5558_21695"
     CDS             2536..3825
                     /gene="glcF"
                     /locus_tag="P5558_21695"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HRY26996.1"
                     /translation="MQTRFTEAQLADPDTRSSEKILRTCVHCGFCTATCPTYVLLGDE
                     LDSPRGRIYLIKNMLEENRPADAKTVKHIDRCLSCLSCMTTCPSGVHYMHLVDHARKH
                     IEATYERPPFERYLRWVLAVVLPRPGLFRIALLKAWLARPLRGLFRGRLRAMLELAPK
                     GGPRPPSPVDKHQTFAAEGTRRGRVALLSGCAQQVLAPEINEATIRLLTRHGVEVVVA
                     RGAGCCGALTHHMGKEAAALASARANVDAWTAEHEREPLDAIIINASGCGTTVKDYGF
                     MLREDAAYAGKAATIAALTKDVSEFLHTQGLMPAVTDTDLVVAYHNACSMQHGQQLKT
                     EAKSLLAQAGFTVKEPAESHICCGSAGVYNIMQPEIAGQLKTRKVRQLERTRPRVIAT
                     GNIGCITQIGSGTDIPICHTVELLDWATGGPKPAALG"
     gene            complement(3830..4147)
                     /locus_tag="P5558_21700"
     CDS             complement(3830..4147)
                     /locus_tag="P5558_21700"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRY26997.1"
                     /translation="MLTAVRAIEAAIGSLTFEEFRADEFRCKAVILDLQILGEAARNL
                     PSSVQAGSPDIPWRNLIDMRNRLAHGYFGIELKIVFDTARLRVPPLAEPIRRLRARLD
                     TGS"
     gene            complement(4180..4485)
                     /locus_tag="P5558_21705"
     CDS             complement(4180..4485)
                     /locus_tag="P5558_21705"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY26998.1"
                     /translation="MTERPGNTEELRSRLAAALPGLRGRYPIRTLGVFGSWARGEQTP
                     ESDVDLLVEFDGVVTYFEVLALEDELRALLGVRVDLATPGALKRFIGERIRSELLPV"
     gene            complement(4595..>4897)
                     /locus_tag="P5558_21710"
     CDS             complement(4595..>4897)
                     /locus_tag="P5558_21710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY26999.1"
                     /translation="GQWEAAASLGLSRFDRMRDVILPQAIRATAPPLANQFISLVKDS
                     SIVSLIAVQELTFMASQTVVTTQRTFEIWLTIGALYFLICWPLSLLFARLEKKTGR"
BASE COUNT          805 a         1819 c         1626 g          647 t
ORIGIN      
        1 ggtggagatt gccgtcgccg gcgtggaaga cattggcgac gcgcagcccg tagccaatgc
       61 ctcccacgcc ggcgacgtca atctccaccc gctcatcctc tacaacgtga acgacccggc
      121 ggacgcgctg aaggccgagg aggccggcaa cgacatcctc gcgctctgcg tcgaggtcgg
      181 cggctgcctc accggcgagc acggcgtcgg catcgagaag cgcgacctga tgcaccgcca
      241 gttcaacgcg gtcgacctcg accagcagct ccgcgtgcgc gccgtcttcg acgcgcaggg
      301 cctgctcaac ccgggcaagg tctttccgct cgacagccgg atcgccgcat gaccctgcac
      361 gagcccgcga gcgaggaaga ggccgccgca ctggtcgccg gcgccaccgc accgcttcgg
      421 ctccagggcg gcggcacgcg ggcgcgaatc ggccggccga tccagacggc ggcaacgctc
      481 accagccgaa gcctcgccgg catcaccctc tacgagccgg ccgagatggt gatcggtgcg
      541 aagagcggca ccccgctcgc cgagatcgag gccaccctcg ccgagcggaa ccagatgctc
      601 accttcgagc ccatggatca ccgcccgctt ttcgccagcg acggcgagcc gaccatcggc
      661 gccgtcgccg ccgccaacat ctccggcccg cgccgcgtct tcggcggtgc ggcgcgcgac
      721 agcctgatcg gcgtgcgctt cgtcaacggc cggggccaga tcatcaagaa tggcggccgg
      781 gtgatgaaga acgtcaccgg gctcgatctg gtcaagctgc aggccggcgc ctacggcacg
      841 ctgggcttcc tcaccgaggt gatcttcaag gtcctgccga ggccggccgc cgccaccacc
      901 ctcgtctatg cccggctcgg cgacgctcgc gccgtggcgc tcatgtgcga agccatgggc
      961 acgcccttcg agatcaccgg cgccgcccac ctgccggccg ggctcggcgc ggtcgaggcc
     1021 cgcaccctgc tccggctcga gggcttcccg gagcagctcg actaccgcgg cggccgtctc
     1081 gccgggcatt tttccgcctt tggcagcccg gagcgcctgg acggcgaggc cggtgccgcc
     1141 ctctggcgcg aggtgcgtga cgtgcggccg ctggtcgagc cttcggatca cgccgtctgg
     1201 cggctcagcc tgccgccgaa ggcgggggcg gccaccgtcg ccgccatccg ccggagcctc
     1261 gccggcgtcg atctccgcgt tctctacgat tggtccggcg gcctcgtctg gctggcccta
     1321 ccggaagcgg acgatgccgg ggcagccgcc atccgcgccg cgacccggga cgcgaagggc
     1381 cacgcgaccc tggtccgggg ctcggccgag ctgcgcgcca ggatcgccgt cttcgagccc
     1441 gagccggccg ccgtggcagc cctcacgcag ggcgtgaaga cgagcttcga tccgcgcggg
     1501 ctgctcaatc ccggcctgat gcatccgggt ggctgagatg cgccgcgccc tcggcccgcg
     1561 agcggacggg cgccttgcgg ccgagccaga cgcagaagac ggtgagcacg gccgcgaccc
     1621 aggtcacgag atcgacccgc tcgcccagca ggagcgcgct gaagccgagc gtgaaataga
     1681 cctgcaggag ctgcatctgc ccgacccggg cgatgccgcc cgccgccagc ccgccatacc
     1741 agaagaagaa gccgatgaac tgcgggaagg ccgcgacgaa gccgagagcg ccccaggcca
     1801 cgaggctggc cgccgccgga tcggccggcc agaggaacag catcagcggc agcgacaccg
     1861 gcaacgccag ggccaggctc caggagatgg cctcgagtgc cggcatgtgc gccgtcatgc
     1921 gcgcaccgcc cgcatagccg atcgaggcgc cgagcccggc cagcgccagg tagagatcgg
     1981 cctgcgagaa cccgcccgcc cgatagagca tgaaggcgag caccacggcg gtgccggcga
     2041 gggcggcgag ccagaagcgc aaggacggcc gctcgccggc gaagagcgcg ccggtgcctg
     2101 ccgtcatgag cggcatcagc gacagcacga cgccgccgtg gctcgcctcc acctcgcgca
     2161 tggcgagggc cgtgcagagc ggaaagccga tcaccacgcc gccgatcacg aggccgaggt
     2221 cgcgccaatg ccgccgcgcc ggcagccggc gccggccgac cagcaggtag gccagcgaca
     2281 ggagcccggc gagggcggcc cggcccgccg tcaccagctc cgcgccgagc tccggcacgg
     2341 cgaggcgcgt gcccggcagg gacaggctga agatgatcac gccgacgagc ccgagcgcat
     2401 agccgcgcct gatcgccgcc gccctgtcca cggtagtctc gtccactgcc gcctcccgct
     2461 tgcccggggt cggctctacg ccccaattgc cgccgtggcg agccgagcgc tcgccaccgg
     2521 tcgagagaaa cgctgatgca gacccgcttc accgaagccc agctcgccga tcccgacacc
     2581 cgctcgtccg agaagatcct tcggacctgc gtgcattgcg gcttctgcac cgccacctgc
     2641 ccgacctacg tgctgctcgg cgacgagctc gacagcccga ggggccgcat ctacctgatc
     2701 aagaacatgc tggaggagaa ccgcccggcg gatgcgaaga cggtcaagca catcgaccgc
     2761 tgcctctcct gcctctcctg catgaccacc tgcccgtccg gcgtgcacta catgcacctg
     2821 gtcgaccatg cccgaaagca catcgaggcg acctacgagc gcccgccctt cgagcgttat
     2881 ctgcgctggg tgctggccgt tgtcctgccg cgcccgggcc tcttccgcat cgcgcttttg
     2941 aaggcctggc tggcccgccc gctgaggggc ctcttcaggg gccggctgcg cgccatgctg
     3001 gagctggcgc cgaagggcgg gccaaggcca ccctcgcccg tcgacaagca tcagaccttt
     3061 gccgcagagg gcacccgccg tggccgcgtc gcgctgctct ccggctgcgc ccagcaggtc
     3121 ctggccccgg agatcaacga ggcgacgatc cgcctgctca cccgccacgg cgtcgaggtg
     3181 gtcgttgcga ggggtgccgg ctgctgcggc gccctcaccc accacatggg caaggaggcc
     3241 gcggccctcg cctcggcccg ggccaatgtc gatgcctgga cggccgagca cgagcgagag
     3301 cccctcgatg ccatcatcat caacgcgtcc ggctgcggca ccaccgtcaa ggactacggc
     3361 ttcatgctgc gcgaggacgc ggcctatgcc gggaaggccg ccaccatcgc cgccctcacc
     3421 aaggacgtca gcgagttcct gcacacacag ggcctcatgc ccgccgtgac cgacaccgac
     3481 ctcgtggtcg cctaccacaa tgcctgctcg atgcagcacg gccagcagct caagaccgag
     3541 gcgaagtcgc tcctcgccca ggccggcttc accgtgaagg agcccgccga gagccacatc
     3601 tgctgcggct ccgccggcgt ctacaacatc atgcagcccg agatcgcggg ccagctcaag
     3661 acacgcaagg tcaggcagct cgagcgcacc cgcccccggg tcatcgccac cggcaatatc
     3721 ggctgcatca cccagatcgg ctccggcacc gacattccca tctgccacac ggtcgagctg
     3781 ctggactggg cgacaggggg gccgaagccg gcggcgttgg ggtgaaaggc taggacccgg
     3841 tgtcgagtcg agcccgcaag cgtcgaattg gctcggcgag cggcggcaca cggaggcgtg
     3901 ccgtatcgaa gacgatcttg agctcgatgc cgaaatagcc gtgcgcgagc ctgttgcgca
     3961 tgtctatcag attgcgccag ggaatgtccg gactgccagc ctggacgctc gacggcaggt
     4021 tgcgggcggc ctcgccaagg atttgcagat cgagaatgac cgccttgcag cggaattcgt
     4081 cagcccgaaa ttcctcgaag gtcaggctgc cgatcgcagc ctcgatggcg cgaacggcgg
     4141 tcagcatgtc gtcgacgaag tccctcgggt cccgccgttt caaaccggca gcagctcgct
     4201 gcgaattcgc tcaccgatga aacgcttgag cgctccgggc gtggccagat cgacccgcac
     4261 accgagcagg gcccgcagtt cgtcctcgag cgccagaact tcgaagtagg tgacgactcc
     4321 atcaaattcg accagcagat cgacgtcgct ctccggcgtc tgctcgccgc gcgcccagga
     4381 gccgaagacg ccgagcgtgc ggatgggata ccggccgcgc agcccgggca gtgccgcggc
     4441 cagccgcgac cgaagctcct cggtattccc cggccgttcc gtcacgtcaa cctgaccctc
     4501 ttcgccgttc gcggcccgac cccacttcta ccacaacgcc gccgcctgcg ccgccggaac
     4561 gttgctgcat gcccgcggat ctgcgctcgg cagcctatcg cccggtcttt ttctccagcc
     4621 gcgcgaagag cagcgaaagc ggccagcata tgagaaagta aagggcgccg atggtcagcc
     4681 agatctcgaa ggtgcgctgc gtcgtcacca cggtctggct ggccatgaag gtcagctcct
     4741 ggacggcgat cagcgagacg atcgagctgt ccttgaccag gctgatgaac tggttggcga
     4801 gcggcggggc cgtggcgcgg atcgcctgcg gcaggatgac gtcgcgcatc cggtcgaagc
     4861 gcgacaggcc gaggctcgcc gccgcctccc actggcc
//
LOCUS       DAOTFI010000305         4892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_20558_length_4892_cov_7.773207, whole genome shotgun sequence.
ACCESSION   DAOTFI010000305 DAOTFI010000000
VERSION     DAOTFI010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20558_length_4892_cov_7.773207"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="P5558_21715"
     CDS             complement(<1..1013)
                     /locus_tag="P5558_21715"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY27000.1"
                     /translation="MTAASGKGPTRIRLGFVGGGRGALIGPVHRTAARLDDRFEVVGA
                     VLSSNAEKSVADAAALGIPKGYGSLGQMIRAGGLDAVAIATPNDSHAALAIEALDAGL
                     HVVCDKPLANTAPDGRRIAEKVAETGLVFCLTHNYSGYPMIRQMRAMIEAGRIGKVHL
                     VQASYRQGTLATAIETGDIPARLRWRLDPAKGGGSHVMGDIGSHVHQLIRYTTGLGID
                     SVMAELGAIVPGRQAHDTGQALLRFANGGRGQMLATKVAHGAENALAIEVHGDQGGLE
                     WRQSRLDELLFLRNGEPQQILTRGSPYLEPLARQACRLPPGHPEGFHEGFGNLYRDFA
                     EQVA"
     gene            complement(1010..2068)
                     /locus_tag="P5558_21720"
     CDS             complement(1010..2068)
                     /locus_tag="P5558_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY27001.1"
                     /translation="MRTIKGPGIFLAQFIAPVAPYDRIETLAPWAAAKGYKGVQVPTF
                     NPAVFDLEQAASSRTWCDEYKGLLAEHGLVISELSTHRQGHCVAVHPAYNLTIDAFTP
                     EAVTRNPEAREAWAIDQLMKAAQASANLGLDRHVTFPGSLLWPYLYLYPPRPFGLIEE
                     GFTELAKRWRPILDRFDELGIDVAYEIHPGEDLHDGLTFEMFLEKLDGHQRVTILYDP
                     SHLHLQGMDYVGFLDVYGERIRAFHVKDAEYRMNARTGYWGGYQNWPTRAGRFRSPGD
                     GQIDFGAIFSKLSELDYDGWASVEWECCIKHPDQGATEAAELIRRHIIQVQERAFDAN
                     MQAQGIDHARNRAALGLK"
     gene            complement(2068..2937)
                     /locus_tag="P5558_21725"
     CDS             complement(2068..2937)
                     /locus_tag="P5558_21725"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY27002.1"
                     /translation="MAGSLSADAVAAYRRDGFHFPWRLMPRERALRIGEDFLAFTRSD
                     VARRYPDPQNQLYLLKAHLIFTWADAIVHDPGLLDAVESLIGPDILVWSSGVFWKAPH
                     SGAYVSWHQDSTNFELDDAEGVVRAWVALTPATLANGTMRFAPGLHARGQVRHVDRKA
                     EGELLSRGETIDLEIDEAATVPVVIDAGEVSFHHLHTPHGSGPNASDQPRVNHVVTYV
                     APKVRPIRGADSAQLARGQDRFGHYAPEPRPKADLDPAALAAHESAMALRNAIIYRGA
                     PLPQAPRHAEVWS"
     gene            complement(2948..3754)
                     /locus_tag="P5558_21730"
     CDS             complement(2948..3754)
                     /locus_tag="P5558_21730"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRY27003.1"
                     /translation="MRQPSGKTRIYAILAHPVAHVRAPEFLNPLFEAAGRDGFIVPFH
                     VRPEDLEDLIPRLLAIRNLGGFIVTIPHKPAMCRMCKTLGPGAKATGTANTIRIEADG
                     SLTGDMFDGLGLVEGCRANGIEPTGKDVLMVGAGGAGRAIAFALAEAGVSRLTIANRT
                     AAKAEEIAADVAKAFPNVRIAAGPADATGHQVIIQTTSLGLKAGDALPMDAATLRPEM
                     QLFDIIAARDTELMKAAEERGLRTVVGGRPMIEHQAKAQIAFLDPPPLPA"
     gene            complement(3767..4636)
                     /locus_tag="P5558_21735"
     CDS             complement(3767..4636)
                     /locus_tag="P5558_21735"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRY27004.1"
                     /translation="MTPDLVLDARAECAESPIWDTGSGRLWWTDIPARRLHRLDPATG
                     RDETFEVPGRVGCFALARTGGLVVAMEHRFARLDPQSGRLETLAEVEPERPENRLNDG
                     RCDRRGRFLAGTMHEPRTIPQGALWQLDPAHGVRLLADHALVGNGLAFSPDDRRMYWS
                     DSRMGRVFIFDYDIDSGSAWNRRLWLETDDSIGRPDGACVDADGCYWSARFKGGRVIR
                     FTPDGRIDREVRLPCSQVTMCAFGGPGLRTLFITTAREGLDAAALAQEPLAGGIFALE
                     AGIAGLPEPEFNA"
     gene            complement(4633..>4892)
                     /locus_tag="P5558_21740"
     CDS             complement(4633..>4892)
                     /locus_tag="P5558_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY27005.1"
                     /translation="SGPGLAATAILCFLYSWNDFFFALILTRTEATTAPVAIVNFMNY
                     EGWAWGKIAAGGAMVMLPVLVFSFLVRRFLIQGMTAGAIKG"
BASE COUNT          705 a         1586 c         1851 g          750 t
ORIGIN      
        1 gccacctgct cggcgaagtc gcggtagaga ttgccgaagc cctcgtggaa gccctcgggg
       61 tggccgggcg gcaggcggca ggcttgccgg gccaggggct cgagataggg gctgccgcgg
      121 gtcaggatct gttgcggctc gccgttcctg aggaagagca gctcgtcgag ccggctctgg
      181 cgccattcga ggccgccctg gtcgccgtgc acctcgatcg cgagcgcgtt ctcggcaccg
      241 tgcgccacct tggtcgccag catctggccg cggccaccat tggcgaagcg gagcagcgcc
      301 tggcccgtgt cgtgcgcctg gcggcccggg acgatggcgc cgagctcggc catgacggag
      361 tcgatcccga gcccggtcgt gtagcggatg agctggtgca catggctgcc gatgtcgccc
      421 atgacgtggc tgccgccgcc cttcgcggga tcgaggcgcc agcgcaggcg ggcggggatg
      481 tcgccggtct cgatggcggt ggccaaggta ccctggcggt agctcgcctg caccagatgc
      541 accttgccga tgcgcccggc ctcgatcatc gcccgcatct ggcggatcat cggatagccg
      601 ctgtagttgt gggtcaggca gaagacgagc ccggtctccg ccaccttctc ggcgatccgc
      661 cggccgtccg gggcggtgtt ggccaagggc ttgtcgcaga ccacgtggag gccggcatcg
      721 agcgcctcga tggcgagggc ggcatggctg tcattgggcg tggcgatggc cacggcatcg
      781 agcccgccgg ccctgatcat ctggccaagc gagccgtagc ccttcgggat gccgagggcg
      841 gcggcatcgg ccaccgactt ttcggcattg gacgaaagca cggcgcccac gacctcgaag
      901 cggtcgtcga gccgggccgc tgtccgatgc acaggaccga tcagggcgcc ccgcccgccg
      961 ccgacgaagc cgaggcgaat gcgggtcggt cccttgccgc tcgccgccgt catttcagcc
     1021 ccagggccgc gcggtttcgg gcatggtcga tgccctgggc ctgcatgttg gcgtcgaagg
     1081 cgcgttcctg gacctggatg atgtggcgcc tgatcagctc ggcggcctcc gtggcgccct
     1141 ggtcggggtg cttgatgcag cactcccatt cgaccgaggc ccagccgtcg tagtcgagct
     1201 cgctgagctt gctgaagatg gcgccgaagt cgatctggcc gtcgccgggg ctgcggaagc
     1261 ggccggcgcg ggtcggccag ttctggtagc cgccccagta gcccgtgcgc gcattcatcc
     1321 ggtactcggc gtccttgacg tggaaggcgc ggatgcgctc gccgtagacg tcgaggaagc
     1381 cgacatagtc catgccctgc aggtgcagat gcgacgggtc atagaggatg gtcacccgct
     1441 ggtggccgtc gagcttctcc aggaacatct cgaaggtcag gccgtcatgc aggtcctcgc
     1501 ccggatggat ctcgtaggcg acgtcgatgc cgagctcgtc gaagcggtcg aggatcggcc
     1561 gccagcgttt cgcgagctcg gtgaagccct cctcgatcag gccgaagggc cggggcgggt
     1621 agaggtagag atagggccag agcagcgagc ccgggaaggt gacgtggcgg tcgagcccga
     1681 gattggccga ggcctgggcc gccttcatga gctggtcgat ggcccaggcc tcgcgcgcct
     1741 ccgggttgcg ggtgacggcc tcgggggtga aggcgtcgat ggtcaggttg taggctgggt
     1801 ggacggcgac gcagtggccc tggcggtggg tcgagagctc ggaaatgacg aggccgtgct
     1861 cggcgagcag gcccttgtac tcgtcgcacc aggtcctgct cgaggccgcc tgctccaggt
     1921 cgaagaccgc ggggttgaag gtcggcacct gcacgccctt gtagcccttg gccgccgccc
     1981 agggggcgag cgtctcgatc cggtcgtagg gggcgacggg ggcgatgaac tgggcgagga
     2041 aaatgcccgg acccttgatc gtgcgcatca gctccagacc tcggcatggc gcggcgcctg
     2101 tggcaatggc gcgccccggt aaatgatggc gttcctgagc gccatggcgg attcgtgggc
     2161 ggccagcgcc gccgggtcga gatcggcctt cggccgcggc tcgggcgcgt agtggccgaa
     2221 gcggtcctgc ccccgggcga gctgggcgct gtccgcgccg cggatcgggc ggaccttcgg
     2281 ggccacgtag gtgacgacgt ggttcacgcg cggctggtcg ctggcattgg ggccggagcc
     2341 gtgcggcgtg tgcagatggt ggaaggagac ctcgcccgca tcgatcacca ccggcacggt
     2401 tgccgcctcg tcgatctcca ggtcgatggt ctcgccgcgc gacaggagct cgccctcggc
     2461 cttccggtcg acatgccgga cctggcccct ggcgtgcagg cccggggcga agcgcatggt
     2521 gccgttggcg agggtcgccg gggtcagcgc cacccaggcg cggacgacgc cctccgcatc
     2581 atccagctcg aaattggtcg aatcctggtg ccaggagaca taggcgcccg aatgcggcgc
     2641 cttccagaag acaccggagg accagaccag gatgtccggg ccgatcaggc tttccaccgc
     2701 gtcgagcagg cccgggtcgt gcacgatggc atcggcccag gtgaagatga ggtgcgcctt
     2761 caggagatag agctggttct gcgggtccgg gtagcgccgg gcgacatccg atcgcgtgaa
     2821 cgccaggaaa tcctcgccga tccgcagcgc gcgttcccgc ggcatcaggc gccaggggaa
     2881 gtggaagccg tcgcggcgat aggcggccac ggcatcggcg gagagggatc cagccatcag
     2941 gcttgcctca tgcgggcaga gggggcgggt cgaggaaggc gatctgcgcc ttggcctgat
     3001 gctcgatcat cggccggccg ccgaccacgg tccgaagccc ccgctcctcg gccgccttca
     3061 tgagctcggt gtcgcgggcc gcgatgatgt cgaagagctg catttccggc ctcagcgtcg
     3121 ccgcatccat cggcagggca tcgcctgcct tcagcccgag cgaggtcgtc tggatgatga
     3181 cctggtggcc cgtggcatcg gccgggcctg ccgctattcg cacgttgggg aaggctttcg
     3241 cgacgtcggc ggcgatctcc tcggccttcg ccgccgtccg gttggcgatg gtcagccggc
     3301 tgacacccgc ctcggcgagc gcgaaggcga tggcgcgccc ggcgccgccg gcgccgacca
     3361 tgagaacgtc cttgccggtg ggctcgatgc cgttggcgcg acagccttcc accagcccca
     3421 gcccgtcgaa catgtcgccg gtcaggctgc cgtccgcctc gatgcggatg gtgttcgccg
     3481 tgccggtggc cttggctccc gggcccagcg tcttgcacat ccggcacatg gccggcttgt
     3541 gcgggatggt gacgatgaag ccgccgagat tgcggatcgc gagaagccgc gggatcaggt
     3601 cctcgaggtc ctccggccgg acgtggaagg gcacgatgaa cccgtcgcgg ccggcggcct
     3661 cgaagagcgg gttcaggaac tcgggggccc ggacatgcgc cacgggatgg gcgagaatgg
     3721 cgtagatcct ggtcttgccg gaaggctggc gcatggtttc ctcggttcag gcgttgaatt
     3781 cgggctccgg caggccggcg atgccggcct ccagggcgaa gatgccgccg gcgagcggct
     3841 cctgggccag ggcggccgcg tcgagcccct cgcgggcggt ggtgatgaag agggtcctca
     3901 ggcccgggcc gccgaaggcg cacatggtca cctgcgagca gggcaggcgg acctcgcggt
     3961 cgatccggcc gtcgggcgtg aagcggatga cccggccgcc cttgaagcgc gcactccagt
     4021 agcagccgtc ggcgtcgacg caggcgccgt cgggccggcc gatgctgtcg tcggtctcga
     4081 gccagaggcg gcggttccag gcgctgccgc tgtcgatgtc gtagtcgaag atgaagacgc
     4141 ggcccatgcg gctgtcggac cagtacatcc gcctgtcgtc ggggctgaag gcgaggccgt
     4201 tgccgaccag cgcgtgatcg gcgagcaggc gaacgccatg cgccgggtcg agctgccaca
     4261 aagcaccctg cgggatcgtg cgtggctcgt gcatggtgcc ggcgaggaag cgcccgcggc
     4321 ggtcgcagcg gccatcgttg aggcggttct ccgggcgctc cggctcgacc tcggcgagcg
     4381 tctccagcct gcccgattgc ggatcgagcc gggcgaagcg gtgctccatg gcgacgacca
     4441 gcccgccggt gcgggcgagg gcgaagcagc cgacgcggcc gggcacctcg aacgtctcgt
     4501 cccggccggt ggcgggatcg aggcggtgca ggcggcgggc cgggatgtcg gtccaccaga
     4561 gccggcccga gcccgtgtcc cagatcgggc tctcggcgca ttccgcccgg gcgtcgagca
     4621 cgagatcggg cgtcatccct tgatcgcccc cgccgtcatg ccctggatca aaaagcggcg
     4681 gacgaggaac gagaagacga ggaccggcag catgaccatg gcgccgccgg cggcgatctt
     4741 gccccaggcc cagccctcgt agttcatgaa gttgacgatg gcgacggggg ccgtggtcgc
     4801 ctcggtccgg gtcaggatga gggcgaagaa gaagtcgttc caggaataga ggaagcagag
     4861 gatcgcggtc gcggcgaggc cggggccgga ga
//
LOCUS       DAOTFI010000306         4821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_20950_length_4821_cov_5.676458, whole genome shotgun sequence.
ACCESSION   DAOTFI010000306 DAOTFI010000000
VERSION     DAOTFI010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20950_length_4821_cov_5.676458"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="P5558_21745"
     CDS             <1..1052
                     /locus_tag="P5558_21745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY27006.1"
                     /translation="IPEEDAEAEAGKTSLIGTGPYKYVEYVPDSHVKLERFEDYSADE
                     RYEEEDGFGGRKTAYFDTVTFRIIPEPGAQVAALEAGEVDLVEQIPMPSAARLESNPD
                     ITLYENMPWAFTTFILNMNEPPTDNLKFREAVQVALNMEEVMGISSEGLFNLNHGWQY
                     PGSTYDAGAIGEEFYNLHDADRAKALLAEAGYNGEKFTILTDTQIPQHGKAAVVIAEQ
                     LNAIGINAVINQVDWPTALKLRLEDTGWNGWTLQMGIEPYLGPVGVIATLTGNRPHFV
                     ENDEKLEALYQELISGETVEARKATFAKIQERLYEIFGIIKIGDAGLMQAARSNLKDF
                     EPFRFTRMYDVWREG"
     gene            complement(1116..2129)
                     /locus_tag="P5558_21750"
     CDS             complement(1116..2129)
                     /locus_tag="P5558_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRY27007.1"
                     /translation="MKAIGYRATGPIDRDDALIDFEMARPSPRGRDLLVRVQAISVNP
                     VDYKIRIRRAPEGDQPAVLGWDAVGEVAGTGDEATLFEPGDMVWYAGAIDRPGTNAEF
                     HLVDERIVGRKPESASAAEAAALPLTTLTAYEMLLDRLRVTDPVPGAAPAVLIIGGAG
                     GVGSIAIQLLRALTDLEVIATASRPETQAWVKELGAHHVVDHTKPLAAQVAAIGIGAP
                     GFVFSTTHTGSYVGEIAELIAPQGRFGLIDDPEALDVMPFKTKCISTHWELMFTRSLF
                     QTADMARQGEILNEVAGLVDAGRVRSTATETLGVINAANLKRAHAILESGKARGKLVL
                     EGF"
     gene            2293..3240
                     /locus_tag="P5558_21755"
     CDS             2293..3240
                     /locus_tag="P5558_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27008.1"
                     /translation="MTRFLIGRLLSAIPTLFVVTLATFLILWLVPGDISAEIGGIDAT
                     PEDLAAIRERLGLDRPLYERALLWYGNLLQGDLGHSYLLNRSVADAVLERLPVTLSLA
                     GLALVIATAIGVLLGILAAVRHNTWLDQGAMVAALIGLSIPDFWFGIVLIILFGVQLG
                     WFPTGGFVPINEDPLGWARCMALPAFTLAITQMGVIARMTRSSMLDVIKQDYVRTARA
                     KGMRRRTVMFRHALGNAMVPIVTVVGVITGVLLSGAVVIEMVFSLPGVGRLIVGAIQR
                     RDYPIIQGGLLVTACVFVFVNIVVDILYGVFDPRVRDGR"
     gene            3230..4144
                     /locus_tag="P5558_21760"
     CDS             3230..4144
                     /locus_tag="P5558_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27009.1"
                     /translation="MAANDAAAETDLSLGAEIARARRRRRTDFLRRLAAHRSFLIGAV
                     ILALFVLVAAFAPLLAPYGPTEINYRAILSPPTATNWFGTDGYGRDIASRVIWGTRVS
                     LRIGLVVVVTTGILGTILGTAASYVDWLDGLIMRTLDGLMAFPGVLLAIALAAALGPS
                     EINAIIALTVTFTPRTARIVRASVLVVQGMQYIEAAHAVGAGHARIIFRYILANALSP
                     LIVQLTFVFAVSIIAEAILSFLGVGPPPPAPSLGNIIADGRSYIQEAWWIAVFPGLVI
                     AIAVLGLNLMGDGLRDVIDPRQRAVGGH"
     gene            complement(4151..>4821)
                     /locus_tag="P5558_21765"
     CDS             complement(4151..>4821)
                     /locus_tag="P5558_21765"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRY27010.1"
                     /translation="GYAMALALVAGVLEARGSGQGRDVDVALYDVGLANLTYLAAWAA
                     NAGHAVERAARSAHPSLVPCQLFRTGDGWIYIMANKERFFPELCSVLGCPELALDPRF
                     SGFAERLVHRAELEQRLDAAFMASNTAAWLERLQGRVPAAPIQDMAEALASPFTRERG
                     MLVDVAGEGGAALKLMGSPFRAGEPVAPRAAPRLGQDTGALLAEAGYDAARIADLRRR
                     GIIG"
BASE COUNT          728 a         1794 c         1536 g          763 t
ORIGIN      
        1 tcatccccga ggaggatgcc gaggccgagg ccggcaagac cagcctgatc ggcaccggcc
       61 cttacaagta tgtcgagtac gtcccggaca gccacgtcaa gctggagcgg ttcgaggact
      121 actcggccga cgagcgctac gaggaggagg acgggttcgg cggcaggaag accgcctatt
      181 tcgataccgt caccttccgc atcatcccgg agccgggtgc ccaggtcgcg gcgctcgagg
      241 ccggcgaggt ggacctcgtc gagcagatcc cgatgccgtc cgcggcacgg ctcgaatcga
      301 accccgacat caccctctac gagaacatgc cctgggcgtt caccacgttc atcctcaaca
      361 tgaacgagcc gcccaccgac aacctgaagt tccgcgaggc ggtgcaggtg gccctgaaca
      421 tggaggaggt catgggcatc tcctcggagg ggctcttcaa cctcaatcac ggctggcaat
      481 atccgggctc gacctacgac gcgggtgcga tcggtgagga gttctacaac ctccacgatg
      541 ccgaccgcgc caaggccctc ctggccgagg cgggctacaa cggcgagaag ttcaccatcc
      601 tcaccgacac ccagatcccg cagcacggca aggcggccgt ggtcatcgcc gagcagctca
      661 acgccatcgg catcaacgcc gtcatcaacc aggtcgactg gccgacggcg ctgaagctgc
      721 ggctcgagga taccggctgg aacggctgga cgctgcagat gggcatcgag ccctatctcg
      781 gccccgtcgg cgtcatcgcc accctgaccg gcaaccgccc gcacttcgtc gagaacgacg
      841 agaagctcga ggcgctctac caggagctca tctcgggcga gaccgtggag gcgcgcaagg
      901 cgaccttcgc gaagatccag gagcggctct acgagatctt cggcatcatc aagatcggcg
      961 acgccgggct gatgcaggcc gcccgctcca acctcaagga cttcgagccc ttccgcttca
     1021 cccgcatgta cgacgtctgg cgcgagggct gacgcaggct gacaggcctg aaagcagcgg
     1081 cggcgcccgc cggggcgccg ccgttttccc ttggctcaga acccttcgag caccagcttg
     1141 ccgcgcgcct tgccgctctc gaggatggca tgcgcccgct tgaggttggc ggcgttgatg
     1201 acacccagcg tctcggtcgc ggtcgaccgg accctgccgg catcgacgag gcccgccacc
     1261 tcgttcagga tctcgccctg gcgagccatg tccgctgtct ggaacagcga gcgcgtgaac
     1321 atcagctccc agtgcgtcga gatgcacttg gtcttgaacg gcatgacgtc cagcgcctcc
     1381 gggtcgtcga tcaggccgaa ccgaccctgc ggtgcgatca gctcggcgat ctcgccgaca
     1441 tagctgcccg tgtgggtggt ggagaagacg aagccgggcg ccccgatgcc gattgccgcc
     1501 acctgcgccg ccaggggctt cgtgtggtcg acgacgtgat gggcacccag ctccttcacc
     1561 caggcctgcg tctcgggccg ggacgccgtc gcgatgacct cgaggtcggt gagtgcgcgc
     1621 aggagctgga tggcgatcga cccgaccccg ccggcaccgc cgatgatcag caccgccggt
     1681 gccgcacccg gcacgggatc ggtgacacgc agccggtcga gcagcatctc gtaggcggta
     1741 agcgtggtca gcggcagggc cgccgcttct gccgccgacg ccgattcggg cttccggccg
     1801 acgatgcgct catccaccag gtggaactcg gcattcgtgc cgggccggtc gatggcaccg
     1861 gcataccaga ccatgtcgcc aggctcgaac agcgtcgcct cgtcgcccgt cccggccacc
     1921 tcgcccacgg cgtcccaccc cagcaccgct ggctggtcgc cctccggcgc gcggcggatg
     1981 cggatcttgt agtcgaccgg gttgaccgag atcgcctgca cccggaccag gaggtcacgt
     2041 cccctggggc tgggcctcgc catctcgaag tcgatgagcg cgtcgtcgcg atcgatgggg
     2101 ccggtggccc ggtagccgat ggctttcatg tcttcccccc gcgcccgtcg ggcgatgccg
     2161 gacctgtcat agcatcgaaa ccaggctatg ccactgcgca cccgccacag gagtcgacgg
     2221 gcgaaaacgg atgccatagc atttgcggca ttcgtgcggc ggggaacgga ggccaggagg
     2281 cgggatgacc gaatgacacg ctttctcatc ggccgcctcc tgagcgccat ccccaccctc
     2341 ttcgtggtca cgctcgccac cttcctgatc ctctggctgg tgccgggcga catcagcgcc
     2401 gagatcggcg gcatcgacgc cacgcccgag gatctcgccg ccatccgcga gcggctcgga
     2461 ctcgaccggc cgctctacga gcgcgctctt ctctggtacg gcaacctcct gcagggcgac
     2521 ctcggccatt cctacctgct caaccgctcg gtcgccgacg cggtgctgga gcgcctgccg
     2581 gtgaccctct cgctggccgg gctggcgctg gtgatcgcca ccgccatcgg cgtgctgctc
     2641 ggcatcctgg cggcggtccg ccacaacacc tggctcgacc agggggccat ggtggcagcc
     2701 ctgatcgggc tctcgatccc ggatttctgg ttcggcatcg tgctcatcat cctcttcggc
     2761 gtgcagctcg gctggttccc gaccggcggc ttcgtgccga tcaacgagga ccccttgggc
     2821 tgggcgcgct gcatggccct gccggccttc actctcgcca tcacccagat gggtgtgatc
     2881 gcccggatga cccgctcctc catgctcgac gtcatcaagc aggactacgt gcgcaccgcc
     2941 cgggccaagg ggatgcgccg ccgcaccgtc atgttccggc atgcgctggg caacgccatg
     3001 gtgcccatcg tcaccgtcgt cggcgtcatc accggcgtgc ttttgagcgg tgcggtggtg
     3061 atcgagatgg tcttctcgct gccgggcgtc ggccggctca tcgtcggcgc catccagcgc
     3121 cgcgactacc cgatcatcca gggcggcctc ctggtcaccg cctgcgtctt cgtcttcgtc
     3181 aatatcgtcg tcgacatcct ctacggcgtt ttcgacccca gggtgcgcga tggccgctaa
     3241 cgacgccgcg gccgagaccg acctctcgct gggcgccgag atcgcccgcg cccggcgacg
     3301 gcggcgcacc gacttcctcc gccgcctcgc cgcacaccgc tcgttcctca tcggtgcggt
     3361 gatcctcgcc ctcttcgtcc tggtcgcggc cttcgccccg ctgctcgccc cctacggccc
     3421 gaccgagatc aactaccggg ccatcctctc gccaccgacc gcgacgaact ggttcggcac
     3481 cgacggctat ggccgcgaca tcgccagccg cgtgatctgg ggcacccgcg tgtcgctccg
     3541 catcggcctc gtcgtcgtcg tcaccaccgg catcctcggc accatcctcg gcacggccgc
     3601 gagctatgtc gactggctcg acggcctgat catgcgcacc ctcgacgggc tgatggcctt
     3661 cccgggcgtg ctcctcgcca tcgcgctggc cgccgccctc ggcccgtcgg agatcaacgc
     3721 catcattgcc ctcaccgtca ccttcacgcc gcgcaccgcg cgcatcgtcc gcgccagcgt
     3781 gctggtggtg cagggcatgc agtacatcga ggcggcccac gccgtgggtg ccggccacgc
     3841 gcgcatcatc ttccgctaca tcctggcgaa cgccctctcg ccgctcatcg tccagctcac
     3901 cttcgtcttc gccgtctcga tcatcgccga ggccatcttg agcttcctcg gcgtcggccc
     3961 accgccgccc gccccctcgc tcggcaacat catcgccgac gggcgcagct acatccagga
     4021 ggcctggtgg atcgccgtct tccccggctt ggtcatcgcc atcgccgtcc tcggcctcaa
     4081 tttgatgggc gacggcctgc gcgacgtcat cgacccccgc cagcgcgccg tcggcgggca
     4141 ttgagcgccc tcaaccgatg atgccacggc gccgcagatc ggcgatccgc gcggcgtcgt
     4201 agccggcctc ggcgagcagc gccccggtgt cctgcccgag ccggggtgcc gcacggggcg
     4261 ccaccggctc gcccgccctg aagggcgagc ccatgagctt gagcgccgcg ccaccctcgc
     4321 cggcgacatc gaccagcatg ccgcgctccc gggtgaaggg cgaggccagc gcctccgcca
     4381 tgtcctggat cggggcggcc ggcaccctgc cctgcagccg ctcgagccac gccgcggtat
     4441 tgcttgccat gaaggcggca tccagacgct gctccagctc ggcccgatgc acgaggcgct
     4501 cggcgaagcc cgagaagcgc gggtccagcg ccagctcggg gcagccgagc acggagcaga
     4561 gctcgggaaa gaagcgctcc ttgttcgcca tgatgtagat ccagccgtcg cctgtgcgga
     4621 agagctggca gggcaccagc gacggatgcg ccgagcgcgc ggcccgctcg accgcatgcc
     4681 ccgcattggc ggcccaggcg gcgagatagg tgagattggc gagccccacg tcatagagcg
     4741 cgacatcgac gtcgcgcccc tgcccgctgc cgcgtgcctc gagcacgccc gcgaccagcg
     4801 ccagcgccat ggcgtagccg g
//
LOCUS       DAOTFI010000307         4809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_21014_length_4809_cov_6.163231, whole genome shotgun sequence.
ACCESSION   DAOTFI010000307 DAOTFI010000000
VERSION     DAOTFI010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21014_length_4809_cov_6.163231"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="P5558_21770"
     CDS             <1..832
                     /locus_tag="P5558_21770"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRY27011.1"
                     /translation="DLRLPEGGVVQPTVLDVEVRGAPPKPDAAARPAPSAGGYVLTLD
                     VLVDMPARIFVRGRGLDSEWGGRLQVAGTSAAPSITGTIDYRRGWLDFLDRRFNIREG
                     TIRFIGETPPNPRITLEAAATAPSMTGIIRVTGRATDPQLTLTSEPQMPQDEVLSRLL
                     FDRDMASLTPMQGLRIANAVGRLEGGGVDAVGELRNLAGLDTLDFGNEDFGAEGTETT
                     ASAGKYVAENVFVAVDQGLSSGTTRARVEVELTPRITLRSEVDDQSRTGVGLEWSMDY
                     "
     gene            859..1695
                     /locus_tag="P5558_21775"
     CDS             859..1695
                     /locus_tag="P5558_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27012.1"
                     /translation="MPEASRPASSLAVGPGTSTFGTFGDLNVIGLWTLYAKEVRRFVK
                     VFTQTLLAPVITTLVFLAIFSLALGADRPAVGGIAFLDFLAPGLIVMAIVQNSFANTS
                     SSIMIAKVQGNIVDYLMPPLSPGELLFGVTMGGVTRGMLVGAAVWLGMLPFVAMSPAH
                     LWAILYFGLMASLLLALLGFLAALWAEKFDHMAAVTNFVITPLSFLSGTFYSIQRLPE
                     PFHMVALANPFFYMIDGVRYGFTGHADASIAVGVAVLALVNGALWLLALRLVRSGYKL
                     KA"
     gene            1784..2971
                     /locus_tag="P5558_21780"
     CDS             1784..2971
                     /locus_tag="P5558_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRY27013.1"
                     /translation="MSQNAAIPSLLPVYSRIDLAFERGEGCWLHTRDGRRFLDFSTGI
                     AVNGLGHCHPHLVQALHDQASRLWHVSNLFRIEQLERLADRLVAHSFAETVFVGNSGA
                     EAMEACIKMARRFQWHRGQAERNRIVTFEGAFHGRTMATISAAGGAKMVDGFAPLLEG
                     FDRVPFGDWAALEAAVTDRTAAVMMEPIQGEGGIRPLERQFLADLRELCDAHDILLVF
                     DEVQCGMGRTGRLFAYEHLGVTPDIMGLAKGLGGGFPIGACVATARAASGMTASTHGS
                     TFGGNPLACAVANAVLDVMLEDGFLDQVNARGAYLREQAEGLAGRHPDEIELVRGTGL
                     MLGIKTRRNNLEFAAELRAAGLLVVPAADNVVRLLPPLVISEAEIDQAIEMIESCCRA
                     RAA"
     gene            2968..3873
                     /gene="argF"
                     /locus_tag="P5558_21785"
     CDS             2968..3873
                     /gene="argF"
                     /locus_tag="P5558_21785"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRY27014.1"
                     /translation="MTGPRHFLDLSAVDAATLRAMLDAAVAMKAGRDRGQPLAGKAVA
                     MIFEKPSTRTRVSFEVGIRQLGGAPIVLNAREMQLGRGETAADTAAVLSRYVDAIMIR
                     TDRHEKLLELAAHATIPVINGLTDASHPCQIMADVLTFEEHRGPIRGRKLAWSGDGNN
                     VATSFIHAAGRFDFELAIACPAELPPDEAALDWARAHQARVSVTTDPFAAVAGADAVI
                     TDTRVSMGDADAERRHRLLEPFAVDARLMAAARPDALFLHCLPAHRGEEVAAEVIDGP
                     QSVVFDEAENRLHAQKAILAWCLGG"
     gene            3884..4807
                     /locus_tag="P5558_21790"
     CDS             3884..4807
                     /locus_tag="P5558_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRY27015.1"
                     /translation="MNVSSTSHLDVMRPFQLEKSQLRGRLVRLGGAVDAVLKAHAYPK
                     PVSDMLGELLVLAGGLAGGLKFDGRFSLQVRSEGVVRLMVADVTNDGQMRGYASFDAE
                     RFDGPDEDPAGFGDLVGSGLMALTVDQAAAGGETYQGIVRLDGRSLRDSMLTYFHQSE
                     QVPTGIRSVVAYDEVAAQWRAAAVIVQAMPEEGSPGERDREEDWRRTMVLLQTASDNE
                     LLDPELAPDTLLYRLFHEDGVRVFEPLDLAFGCSCDDGRVGRMLRRFERDEVLEMRDP
                     SGAVVVTCEFCSRVYRLSDADIFTLFEDSLH"
BASE COUNT          656 a         1735 c         1634 g          784 t
ORIGIN      
        1 cgacctgcgc ctgccggagg gcggggtggt ccagccgacg gtgctcgacg tcgaggtgcg
       61 cggggcaccg cccaagccgg acgcggcggc gcgccccgcc ccgagcgctg gcggctacgt
      121 cctcacgctc gacgtgctcg tcgacatgcc ggcccgcatc ttcgtgcgtg gtcgcggcct
      181 ggattccgaa tggggcgggc ggctgcaggt cgccggcacc agtgccgcgc cgtcgatcac
      241 gggcacgatc gactaccggc gcggctggct cgatttcctc gaccgccggt tcaatatccg
      301 cgagggcacc atccgcttca tcggcgagac cccgcccaat ccgcggatca cgctggaggc
      361 ggccgccacg gcgccgagca tgaccggcat catccgcgtg accgggcgcg ccaccgatcc
      421 gcagctcacg ctcacgagcg agccccagat gccgcaggac gaggtgctgt cgcgcctcct
      481 cttcgaccgc gacatggcga gcctgacgcc gatgcagggt ctcaggatcg ccaacgccgt
      541 gggccggctc gagggcggcg gggtcgatgc ggtaggcgag ctgcgcaacc tcgccgggct
      601 cgacacgctc gatttcggca acgaggattt cggcgccgag ggtaccgaga ccacggccag
      661 cgccggcaag tacgtcgccg agaacgtctt cgtcgcggtc gaccagggcc tcagctcggg
      721 caccacgcgg gcgcgcgtcg aggtcgagct gacgccgcgc atcaccctgc gcagcgaggt
      781 cgacgaccag tcccggaccg gcgtcgggct cgaatggagc atggactact gagcaggatc
      841 ccgcccccgg gcgccgagat gccggaagcg tcgcggcccg cctcgtcgct cgccgtcggc
      901 cccggcacct cgaccttcgg gaccttcggc gatctcaacg tcatcgggct ctggaccctc
      961 tacgccaagg aggtgcgccg gttcgtcaag gtcttcaccc agaccctgct ggcgccggtc
     1021 atcaccacgc tggtgttcct cgccatcttc agcctggcgc tcggcgccga tcgcccggcc
     1081 gtgggcggga tcgcgttcct cgatttcctc gccccggggc tgatcgtcat ggcgatcgtc
     1141 cagaactcct tcgccaacac ctcgtcctcg atcatgatcg ccaaggtcca gggcaatatc
     1201 gtcgactacc tgatgccgcc cttgtcgccc ggtgagctgc tcttcggcgt gaccatgggc
     1261 ggggtcacgc gcggcatgct ggtgggcgcc gccgtctggc tcggcatgct gcccttcgtg
     1321 gcgatgagcc cggcccatct ctgggccatc ctctatttcg gcctgatggc gtcgctgctg
     1381 ctggcgctat tgggcttcct cgcggcgctc tgggcggaga agttcgacca catggcggcg
     1441 gtgacgaact tcgtcatcac cccgctctcg ttcctgtccg gcaccttcta ctccatccag
     1501 cgcctgccgg agccgttcca catggtggcg ctggccaacc ccttcttcta catgatcgac
     1561 ggggttcgct acggcttcac cgggcatgcc gatgccagca tcgcggtcgg cgtcgctgtc
     1621 ctcgccctcg tcaatggcgc tctctggctc ctggcgctcc gcctcgtccg ctcgggctac
     1681 aagctcaagg cctagggcgc acaaggttcg gccgcgttga cgcgttcctt cgggtcggcc
     1741 atcttccgcg ctctttcccc cgaccccagc ggaccactcg acgatgagcc agaacgccgc
     1801 gattccctcg ctcctgcccg tctacagccg gatcgatctc gccttcgagc gaggcgaggg
     1861 ttgctggctc catacccgcg acggccgccg cttcctcgat ttctcgaccg gcatcgcggt
     1921 caatggcctc ggccactgcc acccgcatct ggtccaggcc ctgcacgacc aggccagccg
     1981 gctctggcac gtctccaatc tcttccgcat cgagcagctc gagcggctgg ccgaccggct
     2041 ggtcgcccac tccttcgccg agacggtctt cgtcggcaat tccggcgccg aggcgatgga
     2101 ggcctgcatc aagatggcgc ggcgcttcca gtggcatcgc ggccaggcgg agcgcaaccg
     2161 catcgtcacc ttcgagggcg ccttccacgg ccgcaccatg gcgaccatct cggcggccgg
     2221 cggtgccaag atggtggacg gcttcgcgcc cctgctggaa ggcttcgacc gggtgccctt
     2281 cggcgactgg gcggcgctcg aggcggcggt caccgaccgc accgcggcgg tgatgatgga
     2341 gccgatccag ggcgagggcg gcatccgccc gctcgagcgg cagttcctgg cggacctgcg
     2401 cgagctctgc gacgcccatg acatcctcct cgtcttcgac gaggtgcagt gcggcatggg
     2461 ccgcaccggc cggctcttcg cctatgagca tttgggcgtg accccggaca tcatgggcct
     2521 cgccaagggt ctcggcggcg gcttccccat tggcgcctgc gtcgccaccg cccgtgccgc
     2581 ctccggcatg accgcctcga cgcacggctc gaccttcggc ggcaacccgc tcgcctgcgc
     2641 ggtcgccaac gcggtgctcg acgtcatgct cgaggacggc ttcctcgacc aggtgaacgc
     2701 gcgcggcgcc tatctgcgcg agcaggcaga ggggctcgcc gggcgccatc ccgacgagat
     2761 cgagctggtg cggggcacag ggttgatgct cggcatcaag acgcgccgca acaacctgga
     2821 gttcgccgcc gagttgcgcg cggccgggct cctggtcgtg cccgccgccg acaatgtcgt
     2881 ccgcctgctg ccgcctctgg tcatcagcga ggccgagatc gaccaggcga tcgagatgat
     2941 cgaatcctgt tgccgggccc gggcggcgtg accgggcctc gtcacttcct cgatctctcg
     3001 gcggtcgacg ccgccacgct gcgcgccatg ctggacgccg cggtggcgat gaaggcgggg
     3061 cgcgacaggg gccagccgct cgccggcaag gccgtggcga tgatcttcga gaagccttcg
     3121 acccgcacgc gcgtctcgtt cgaggtcggc atccgccagc tcggcggggc gccgatcgtg
     3181 ctcaatgcca gggagatgca gctcggccgc ggcgagacgg cggccgatac cgcggccgtg
     3241 ctgtcgcgct atgtcgatgc gatcatgatc cgcaccgacc ggcacgagaa gctgctggag
     3301 ctcgcggcgc atgccacgat cccggtgatc aacggcctga ccgatgcctc gcatccctgc
     3361 cagatcatgg ccgacgtgct caccttcgag gagcaccggg ggccgattcg gggcaggaag
     3421 ctcgcctggt ccggcgacgg caacaatgtc gcgacctcct tcatccatgc cgccggccgc
     3481 ttcgacttcg agctcgccat cgcctgcccc gccgagctgc cgcccgatga ggcagcgctc
     3541 gactgggcga gggcgcatca ggcgcgggta tcggtcacga ccgatccttt tgcggcggtg
     3601 gccggcgccg atgccgtcat caccgacacg cgggtctcga tgggcgacgc cgacgccgag
     3661 cgccgccacc gcctgctcga gcctttcgcg gtcgatgccc ggctcatggc ggcggcccgg
     3721 ccggatgcgc tcttcctcca ttgcctgccg gcacaccgcg gcgaggaggt ggcggccgag
     3781 gtcatcgacg ggccgcagag cgtggtcttc gacgaggcgg aaaaccggct gcacgcccag
     3841 aaagccattc tcgcctggtg cctgggcggg tagcatcgtc gccatgaacg tttcatcgac
     3901 ctcccatctc gacgtcatgc ggccttttca gctcgagaaa tcgcagctgc gcgggcggct
     3961 ggtgcggctc ggcggcgcgg tcgacgccgt gctcaaggcg catgcctatc ccaagccggt
     4021 ctccgacatg ctgggcgagc ttctggtgct cgccggcggg ctggccggcg ggctcaagtt
     4081 cgacggccgc ttctcgctgc aggtgcgcag cgagggcgtc gtccgcctga tggtggcgga
     4141 cgtcaccaat gacgggcaga tgcgcggcta tgcgagcttc gatgccgagc gtttcgacgg
     4201 gcccgacgag gaccccgccg ggttcggcga tctggtcggc tcgggcctca tggcgctgac
     4261 cgtcgaccag gcggcggccg gcggcgagac ctaccagggc atcgtccggc tggacggccg
     4321 ctcgctccgc gacagcatgc tgacctattt ccaccagtcc gagcaggtgc cgaccggcat
     4381 ccgctcggtc gtcgcctatg acgaggtggc ggcccagtgg cgggcggccg ccgtcatcgt
     4441 gcaggcgatg cccgaggaag gctcgccggg cgagcgggac cgcgaggagg actggcggcg
     4501 cacgatggtg ctcctgcaga cggcgagcga caacgagctg ctcgacccgg agctggcgcc
     4561 cgacacgctg ctctatcgcc tcttccacga ggacggcgtg cgggtgttcg agccgctcga
     4621 cctcgccttc ggctgcagct gcgacgacgg ccgggtcggc cggatgctgc gccgcttcga
     4681 acgcgacgag gtcctggaga tgcgcgatcc cagcggtgcg gtggtcgtca cctgcgagtt
     4741 ctgcagccgg gtctaccggc tgagcgacgc cgatatcttc accctgttcg aggacagcct
     4801 gcattgagc
//
LOCUS       DAOTFI010000308         4773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_21210_length_4773_cov_7.757948, whole genome shotgun sequence.
ACCESSION   DAOTFI010000308 DAOTFI010000000
VERSION     DAOTFI010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21210_length_4773_cov_7.757948"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="P5558_21795"
     CDS             complement(<1..848)
                     /locus_tag="P5558_21795"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRY27016.1"
                     /translation="MRLSRYFLPVLKETPAEAQIVSHQLMLRAGMVQQLAAGIYSWLP
                     LGYAVLRKVEAIVHQEMQRAGAIPILMPTIQPADLWIESGRYNDYGKEMLRIRDRHDR
                     SMLYGPTNEENVTDIFRRHVRSFKQLPLNLYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDLDAAGAEASYDAMFLAYLRTFRRMGLTAIPMQADTGPIGGNLSHEFQILARTGE
                     SEVHFDAAFEAIDFSADDLSVTELKALYAAADDKHDPAACPVPAERLRSGRGIEVGHI
                     FYFGTK"
     gene            complement(956..4168)
                     /locus_tag="P5558_21800"
     CDS             complement(956..4168)
                     /locus_tag="P5558_21800"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY27017.1"
                     /translation="MSAVDSASLLRLATRLTGLPVALLVRLEGCNLQIVGEPHAGQQA
                     RVDLGPLLRRLLDGRQRGLIRAVGAAHGSARPEDQVAARLAACGFGSAVVAPLPDPGG
                     AASRMLCCLGPPGVTPEPTAADALADLAGLAAAMENARRATERAEAGEARLRAAIDSI
                     PHHFWVTDAEGRYVLQNAEDRATYGDLIGLRADQSGMPAEIIDEWRDRHRRVLAGETL
                     RFAGAKCIGEREIVAERLIAPLVIDDVIVGQVGLAVDITARVQTERRLAESEARLRAA
                     VDALPFPFFICDLDGRHILQNARDREVWGDGIGKTFAELGLPAAIVASLPEAIERIRA
                     GEARHKLVCYRHGGELRHVEEVYAPVEANGTITGFVGLAIDHTDRVLTEQRLQESEAR
                     LRDYLATASDWLWETDAAHRFIRGMGELRRSGLRLDDLIGESLQELVEDDPARECYGE
                     EHRADLAHGRQFRDRLVRRADGTGREVWLEVSGNPLLDSAGRLIGYRGTARDVTDRVA
                     AEVALREAHARLEALAASGLIGITAGRGWLVEEGNDAFLRMLGRHRAELEAGALDWRE
                     LADDQGGRPVEERRGGAVTGTMIAVEQELVRSDGSRVPVLLNRVVLHAAEERWFALVQ
                     DLTTVKAAEQRIRMLAERDGLTGLANRRVLFERLQGDLADRRRPGDLGALLMLDLDHF
                     KEVNDRLGHNAGDALLRAVGERLTRVLRDTDTIARLGGDEFAMVLRDLQGPAAAADIA
                     TKLLAVLGEPLEFEGQLLQPRCSIGISLFPRDGADVMRLLKNADIALYQAKAKGRGRF
                     CFFEPALLEAIERRQAIGEALQAGIAEKAFEIELQPQIHLASGCHAGFEALVRWRRDG
                     ELVAPDEFIGIAEEVGVIASLGRLVLRHALEALRRCDDLGLDAGHVAVNLAAAQLRAP
                     GFPAEVAALLGELGLEAGRLELEVTEGVLLDRDAVAISTSLRELHGLGVTITLDDFGT
                     GAASLAHLKSFPVDRLKIDRSFVRGIGRNADDAVFVCTIINLAHTLGMVVVAEGVETA
                     EQEAFLELHGCDLAQGFRFARPMPADGIAAYLAPTGVLAR"
     gene            complement(4559..4771)
                     /locus_tag="P5558_21805"
     CDS             complement(4559..4771)
                     /locus_tag="P5558_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HRY27018.1"
                     /translation="MRSFIERVERLEEEKSEVQEQIKEVLSEAKGEGFDVKILRQLLR
                     LRKMKPHDRAEQEELLETYKAAIGMV"
BASE COUNT          741 a         1677 c         1617 g          738 t
ORIGIN      
        1 tacttggtgc cgaaatagaa gatgtggccg acctcgatgc cgcggccgct gcgcagccgc
       61 tcggctggca ccgggcaggc ggccgggtcg tgcttgtcgt cggcggcggc atagagggcc
      121 ttgagctcgg tcaccgagag gtcgtcggcc gaaaagtcga tggcctcgaa ggccgcatcg
      181 aaatggacct cgctctcgcc ggtcctggcg agaatctgga actcgtggct caaattgccg
      241 ccgatcggac cggtatcggc ctgcatcgga atagccgtca ggcccatccg gcggaaggtg
      301 cgcaggtagg ccaggaacat ggcgtcatag gaagcctccg cacccgccgc gtccagatcg
      361 aagctgtagg cgtccttcat caggaactcg cggccgcgca tgacgccgaa gcgcgggcga
      421 acctcgtccc tgaacttcca ctggatatgg tagaggttga gcggcagctg cttgaagctc
      481 cggacgtgcc ggcggaagat gtcggtgacg ttctcctcgt tggtcggccc gtagagcatg
      541 ctgcgatcgt ggcggtcccg gatgcgcagc atctccttgc catagtcgtt gtagcggccg
      601 ctctcgatcc agagatcggc cggctggatg gtcggcatca ggatggggat ggcgccggcg
      661 cgctgcatct cctggtggac gatcgcctcg accttgcgca ggacggcgta gccgagcggc
      721 agccagctgt agatgccggc ggcgagctgc tggaccatgc ccgcacgcag catgagctga
      781 tggctcacga tctgcgcctc ggcgggtgtt tccttaagca cgggcaggaa atagcgtgac
      841 aggcgcatgc gcaactcgtt cgttctgcgg aaaggtgggg atcaagcgga aagccgagcg
      901 cctgatggca cgctttgccg gcaggcgcaa cgtccgcggc gggcggcgga acggcctagc
      961 gcgccagcac ccccgtcggt gccaggtagg cggcgatgcc gtccgccggc atcggccggg
     1021 cgaaacggaa gccctgcgct agatcgcagc cgtgcagctc gagaaaagcc tcctgctcgg
     1081 cggtctcgac gccctccgcg acaacgacca tgccgagcgt gtgggcgaga ttgatgatcg
     1141 tgcagacgaa gacggcatcg tctgcgttgc ggccgatgcc gcgcacgaac gagcggtcga
     1201 tcttcagccg atcgaccggg aagctcttga ggtgagcgag cgaagcggcc ccggtgccga
     1261 aatcgtccag cgtgatggtc acgccgaggc catgcagctc gcgcaggctc gtcgatatcg
     1321 ccacggcgtc gcggtcgagc aggacaccct cggtcacctc gagctccagc cgtcccgcct
     1381 cgagcccgag ctcgccgagc aaggctgcca cctccgccgg gaagcccggt gcccggagct
     1441 gggccgcggc gagattgacc gcgacatggc cggcatccag gccgagatcg tcgcaacggc
     1501 ggagtgcctc cagggcatgg cgcagcacca gccggccgag cgatgcgatg acgccgacct
     1561 cctcggcgat cccgatgaac tcgtcgggcg ccacgagctc gccgtcgcgt cgccagcgga
     1621 ccagcgcctc gaaaccggcg tggcagccgc tggccaggtg gatctgcggc tgcagctcga
     1681 tctcgaacgc cttctcggcg atgcctgcct gcagcgcctc gccgatcgcc tgtcgtcgct
     1741 cgatcgcctc gagcagcgcc ggctcgaaga agcagaagcg gccgcggcct ttggccttgg
     1801 cctggtagag ggcgatgtcg gcattcttga ggagacgcat cacgtcggcg ccgtcgcgcg
     1861 gaaacaggct gatgccgatg ctgcagcgcg gctgcaggag ctggccctcg aactcgagcg
     1921 gctcgcccag cacggcgagc agcttggtcg cgatatcggc agcggcggcc ggtccctgca
     1981 ggtcgcgcag caccatggcg aattcgtcgc cgccgagccg ggcgatcgtg tcggtatccc
     2041 gcagcacccg ggtcagccgc tcgcccactg cccgcagcag cgcatcgccc gcgttgtggc
     2101 cgagccggtc gttgacctcc ttgaagtgat cgagatcgag catcaggagg gcacccaggt
     2161 cgcctggtcg gcgacggtcg gcgagatcgc cctgcagccg ctcgaacagc acccgacggt
     2221 tggcgagccc ggtgaggcca tcgcgctcgg cgagcatccg gatccgctgc tccgccgctt
     2281 tcaccgtggt caggtcctga accagcgcga accagcgctc ctcggccgca tgcaacacga
     2341 cgcgattgag cagcaccggc acgcggctcc cgtcggaacg gacgagctcc tgctcgaccg
     2401 cgatcatggt ccccgtcacc gccccgccac gccgctcctc gaccgggcgc cccccttgat
     2461 cgtcggccag ctcccgccag tcgagcgcac cggcttcgag ctcggcccgg tggcggccga
     2521 gcatccgcag gaacgcgtcg ttgccctcct cgaccagcca gccccggccc gcggtgatgc
     2581 cgatcagtcc ggaggcggcg agcgcctcca gccgggcatg cgcctcgcgc aaggccacct
     2641 cggccgcgac ccggtccgtg acgtcgcgcg ccgtgccgcg atagccgatc aaccgacccg
     2701 ctgaatcgag cagcggattg ccgctcacct ccagccagac ttcgcggccc gtgccgtcgg
     2761 cgcgcctgac gaggcgatcg cgaaactgtc gcccgtgggc gaggtcggcc cggtgctcct
     2821 cgccgtaaca ttcgcgggcc gggtcgtcct cgaccagctc ctgcagcgac tcgccgatga
     2881 ggtcgtccag ccggaggccg gaccgccgca actcgcccat gccacgaatg aagcggtgcg
     2941 cggcatcggt ctcccagagc cagtccgagg cggtcgccag gtagtcgcgc agcctggcct
     3001 cgctctcctg cagtcgttgc tcggtcagga cccggtcggt gtggtcgatg gcgagtccga
     3061 cgaagccggt aatcgtgcca ttggcctcga ccggtgcgta gacctcctcg acatggcgca
     3121 gctcgccgcc gtgccggtag cagacgagct tgtgccgggc ttcgcccgcg cggatccgct
     3181 cgatcgcctc cggcaggctc gcgacgattg ccgccggcag cccgagctcg gcgaaggtct
     3241 tgccgatccc gtcgccccat acttcgcggt ccctggcgtt ctgcaggatg tggcggccgt
     3301 cgagatcgca gatgaagaac ggaaacggca gcgcgtcgac tgccgcccgc aagcgggcct
     3361 cgctctcggc gagccgccgc tccgtctgca cgcgcgccgt gatgtcgacc gcaaggccca
     3421 cctggcccac gatgacgtcg tcgatgacca ggggggctat cagccgctcg gcgacgattt
     3481 cgcgttcgcc gatgcacttg gcaccggcga agcggagggt ctcgccggcg agcacgcgcc
     3541 ggtgtcggtc acgccactcg tcgatgatct cggctggcat gccactctgg tcggcgcgca
     3601 gcccgatgag gtcgccatag gtggcgcggt cctccgcgtt ctgcaggacg tagcggccct
     3661 cggcgtcggt gacccagaag tggtgcggga tgctgtcgat agccgcccgc aggcgcgcct
     3721 cgcctgcctc ggcgcgctcg gttgcgcgcc tcgcattttc catcgccgcc gcgagcccgg
     3781 caagatcggc gagcgcatcc gcggcggtgg gctcgggcgt cacccccggc gggccgaggc
     3841 agcagagcat tcgagaggct gccccgccgg gatcgggtag cggggccacg accgccgagc
     3901 cgaagccgca tgcggcgagc cgggcggcga cctgatcctc ggggcgggcg cttccgtgag
     3961 ccgcgccgac ggcccggatc aggccccgct gccggccatc cagcagcctt cgcagcaggg
     4021 gcccgagatc gacccgagcc tgctgtcccg cgtgcggttc gccgacgatc tgcaggttgc
     4081 agccttcgag gcgcaccagc agggcgacag gcaacccggt gagccgcgtg gcaagtcgca
     4141 acagcgaggc gctatcgact gccgacatgt cttagggcct gtccgagaag ttcatggggc
     4201 ctttcattgg cttacgatcg accgccggcg cggagccgag cgcggcccga tgccggggca
     4261 tcgggcaagc tcggcgacaa agccggcgcc gatcgcaagc cgaccggagg tgagcttggc
     4321 agcggcacct gcttcgtcgt tgatcgtccg acgatgcgct gcatcgacga acgatcaact
     4381 cctcgcatct gctcactgcc aagctcatga aaggcctcat gaacttctcg gacaggctct
     4441 tattccccgc cgccgccccc agccgtcaag ccacgtcatt ccgaccacca tcgcagtgga
     4501 tcgaaagcat aggcggcaat tgccaagatt tggctaatgc ggcagccccg gcgcggcctc
     4561 agaccatgcc gatcgcggcc ttgtaggtct ctagcagctc ctcctgctcg gcgcggtcgt
     4621 gcggcttcat cttccgcaga cgcaagagct ggcgcaggat cttgacgtcg aagccctcgc
     4681 ccttcgcctc gctcagcacc tccttgatct gctcttgaac ctcgctcttc tcctcctcga
     4741 gacgctcgac ccgctcgatg aagctgcgca ggc
//
LOCUS       DAOTFI010000309         4771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_21221_length_4771_cov_6.978584, whole genome shotgun sequence.
ACCESSION   DAOTFI010000309 DAOTFI010000000
VERSION     DAOTFI010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21221_length_4771_cov_6.978584"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1741
                     /gene="rpsA"
                     /locus_tag="P5558_21810"
     CDS             11..1741
                     /gene="rpsA"
                     /locus_tag="P5558_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRY27019.1"
                     /translation="MQEAIATTQAPSQADFASMLDESLGGSSKLEGSVVKGTVIALDN
                     EDAVVDVGLKSEGRVPIKEFSTPGHPVEVKVGDEVEVYVERFENRSGEAVLSREKARR
                     EESWNRLERAFNEQDKVEGAIFGRVKGGFAVDLGGAVAFLPGSQVDIRPVRDVAPLVN
                     KPEPFLILKMDRRRGNIVVSRRAVLEESRKEQRDELLATLSEGQILDGVVKNITDYGA
                     FVDLGGLDGLLHVTDISWKRVNHPTEVLGVGAQVKVQVIRFNRETQRISLGMKQLEAD
                     PWEGVELKYPVGTKFKGTVTNITDYGAFVELEPGVEGLVHVSEMSWTKKNIHPGKIVS
                     TSQEVEVMVLEVDPEKRRISLGLKQTLANPWDAFLEAHPVGSTVEGEIRNITEFGLFV
                     GVDGEVDGMVHLSDIDWEKPGEEAVQAFSKGDVVKAVVLDVDIEKERISLGMKQLSDD
                     PFKEATSGIKKGQRVTCTVKEITTGGIEVETSTGAVGFIRKAELSRDRDEQRPERYAV
                     GEKLDARVINIDAASRRLTLSVKALEIADEKQAMAEYGSSDSGASLGDILGAALRQKQ
                     EEAKPDDGDK"
     gene            1900..2181
                     /locus_tag="P5558_21815"
     CDS             1900..2181
                     /locus_tag="P5558_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HRY27020.1"
                     /translation="MTKSDLIKRLAEANPHLFLRDIERIVSTVFDGIGEALAEGDRVE
                     LRGFGAFSVRPRGERTGRNPRTGDEVQVPKKLVPYFKTGKELRERLNKS"
     gene            2232..2585
                     /locus_tag="P5558_21820"
     CDS             2232..2585
                     /locus_tag="P5558_21820"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRY27021.1"
                     /translation="MAKLLRTVVGLLGLLVIVVFSVANRGIVDVSFFPLPIPTIPLPV
                     YAVFLIGIFLGAIIGGLALWLSEHRMRAEWRRLRRKARAEAHQRQLKELEEERVAAER
                     SRQRQDALALAAPRR"
     gene            2603..3568
                     /locus_tag="P5558_21825"
     CDS             2603..3568
                     /locus_tag="P5558_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRY27022.1"
                     /translation="MPLLDRAAVDAALPFPDLIEALRAGFALGCEAPVRHHHTVPRKH
                     APDATLLIMPAWQQSRRLGVKVVQISPGNDALGLPTVDGLYLLFEAHTGRPLAVMNGP
                     AITERRTAAASALAASHLAREDARHHLVLGAGAVAAMLVPAHAAIRPIERVTLWNRTT
                     ARADKLAERLTTEGFDVAVTTDLDGALGEADIVSAATMSAVPLVKGALVRPGTHVDLV
                     GAYNRALRESDDALMTRASLFVDTRGGALGEAGDIVQAIASGAIDEAAIQADLAELCR
                     GDHPGRRTAEEVTVFKSVGAAIEDLVAAELVYERLERSRDPGSGR"
     gene            3573..3953
                     /locus_tag="P5558_21830"
     CDS             3573..3953
                     /locus_tag="P5558_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27023.1"
                     /translation="MLGFLGRFGRAEGLRQLDAALRAVDLHPALVPEAVKLTTLRQLQ
                     QEAGGKPRPADFAAAAELLAYCMTGPEAFAQATDPTLTAAVETRIEAAIQAGDSLDAR
                     LVLLTLHAGLTQPAVVARFGLEAG"
BASE COUNT          828 a         1708 c         1484 g          751 t
ORIGIN      
        1 tggagcaaga atgcaggaag ccatcgcgac cacccaagcc ccgtcccagg ccgatttcgc
       61 gtcgatgctc gacgagagcc tgggcggcag ctcgaagctg gaaggctcgg tggtcaaggg
      121 cacggtcatc gccctcgaca acgaggacgc ggtcgtcgat gtcggcctca agtccgaagg
      181 ccgcgtgccc atcaaggaat tctcgacccc cggccacccc gtcgaggtga aggtcggcga
      241 cgaggtcgag gtctatgtcg agcgcttcga gaaccgcagc ggcgaggccg tcctcagccg
      301 cgagaaagcc cgccgcgagg agagctggaa ccggctcgag cgggcgttca acgagcagga
      361 caaggtcgag ggcgccatct tcggccgggt caagggcggc ttcgccgtcg acctcggcgg
      421 ggccgtggcc ttcctgccgg gcagccaggt cgacatccgc ccggtgcgcg acgtcgcccc
      481 gctcgtcaac aagcccgagc ccttcctcat cctcaagatg gaccgccgcc gcggcaacat
      541 cgtcgtctcg cgccgggccg tgctcgagga gagccggaag gagcagcgcg acgagctgct
      601 cgccaccctc tccgagggcc agatcctcga cggcgtggtc aagaacatca ccgactacgg
      661 cgccttcgtc gacctcggcg gcctcgacgg cctcctgcac gtcaccgaca tctcctggaa
      721 gcgcgtcaac cacccgaccg aggtcctcgg cgtcggcgcc caggtcaagg tgcaggtcat
      781 ccgcttcaac cgcgagaccc agcgcatcag cctcggcatg aagcagctcg aggccgatcc
      841 ctgggagggc gtggagctca agtacccggt cggcaccaag ttcaagggca ccgtcaccaa
      901 catcaccgac tacggcgcct tcgtcgagct ggagcccggg gtcgagggcc tggtccacgt
      961 ctccgagatg agctggacca agaagaacat ccatccgggc aagatcgtct cgaccagcca
     1021 ggaggtcgag gtgatggtgc tcgaggtcga tcccgagaag cgccgcatca gcctcggcct
     1081 gaagcagacc ctggccaacc cctgggatgc cttcctcgag gcgcaccccg ttggctcgac
     1141 ggtcgagggc gagatccgca acatcaccga gttcggcctc ttcgtcggcg tcgacggcga
     1201 ggtggacggc atggtccatc tctccgacat cgactgggag aagcccggcg aggaggcggt
     1261 ccaggccttc agcaagggcg acgtggtcaa ggccgtggtg ctcgatgtcg acatcgagaa
     1321 ggagcgcatc agcctcggca tgaagcagct ctcggacgac cccttcaagg aggccacctc
     1381 cggcatcaag aagggccagc gggtcacctg caccgtcaag gagatcacca ccggcggcat
     1441 cgaggtcgag acctcgaccg gggcggtcgg cttcatccgc aaggcggagc tcagccgcga
     1501 ccgcgacgag cagcggcccg agcgctacgc ggtcggcgag aagctcgatg cccgggtgat
     1561 caacatcgac gccgccagcc gccgcctgac cctctcggtc aaggcgctcg agatcgccga
     1621 cgagaagcag gcgatggccg agtatggctc ctccgacagc ggcgccagcc tcggcgacat
     1681 cctgggcgct gccctgcgcc agaagcagga ggaggcgaag cccgacgacg gcgacaagta
     1741 gcgtcgtcga ccgctccttc gggcaccaga cggacgccgc cgggccacca cccggcggcg
     1801 tccggcgttg gccggcgcgt caggccgtcc cttgacgcta cccctcgcct ttgccaagct
     1861 ccgcctgcag cttgcggcct cggccgggta ggaagcgcca tgaccaagtc ggatctcatc
     1921 aagcgcctcg ccgaggcgaa cccgcacctc tttctccgcg acatcgagcg gatcgtgtcg
     1981 accgtgttcg acggcatcgg cgaggccctc gcggaaggcg accgcgtcga gcttcgcggc
     2041 ttcggcgcct tctcggtgcg cccgcgcggc gagcggaccg gccgcaaccc gcgcaccggc
     2101 gacgaggtgc aggtgccgaa gaagctcgtg ccgtacttca agaccggcaa ggagctgcgc
     2161 gagcggctca acaagagctg aactgaaccg ctttcttcat cacccccctc ttcaacacga
     2221 ggaccgcccc catggcgaaa ctcctgcgaa ccgtggtcgg gctgctcggc ctgctcgtca
     2281 tcgtcgtctt ctcggtcgcc aatcgcggca tcgtcgacgt cagcttcttc cccctcccca
     2341 tcccgacgat cccgctgccg gtctatgccg tcttcctgat cggcatcttc ctcggcgcca
     2401 tcatcggcgg cctggcgctc tggctctccg agcaccggat gcgcgccgaa tggcgccggc
     2461 tccgccgcaa ggcacgcgcc gaggcgcacc agcggcagct caaggagctg gaggaggagc
     2521 gcgtcgccgc cgagcgctcc cgccagcgcc aggacgcgct cgccctggcg gcgccccggc
     2581 gctgagcgct gccacaccgg cgctgccgct cctcgaccgc gcggcggtcg acgcggccct
     2641 gcccttcccg gacctgatcg aagcgctccg cgccggcttc gccctgggct gcgaggcgcc
     2701 ggtccgccac caccacaccg tcccccgcaa gcacgccccg gacgccaccc tgctcatcat
     2761 gccggcctgg cagcaaagcc ggcggctcgg cgtcaaggtc gtgcagatct cgcccggcaa
     2821 cgacgcgctc ggcctgccga cggtcgacgg gctctatctc ctcttcgagg cccataccgg
     2881 ccggccgctc gcggtgatga acggcccggc gatcaccgag cgccgcaccg ccgcggcctc
     2941 ggccctggcc gcgagccatc tcgcccggga agatgcccgc caccacctcg tgctcggcgc
     3001 cggtgccgtc gccgccatgc tggtcccggc ccatgccgcc atccggccga tcgagcgggt
     3061 gacgctctgg aaccggacga cggcccgcgc cgacaagctg gccgagcggc tgaccaccga
     3121 aggcttcgac gtcgccgtga ccaccgacct cgacggggcg ctcggcgagg ccgacatcgt
     3181 ctcggccgcg accatgagcg ccgtgccgct cgtcaagggt gccctcgtcc gccccggcac
     3241 ccatgtcgac ctagtcggcg cctacaatcg agccttgcgc gagagcgacg acgcgctcat
     3301 gacccgcgcc agcctcttcg tcgacacccg cggcggtgcg ctcggcgagg ccggcgacat
     3361 cgtccaggcg atcgccagcg gcgccatcga cgaggccgcc atccaggccg atctcgccga
     3421 gctctgccgc ggcgaccatc ccggccgccg cacggccgag gaggtgacgg tcttcaagtc
     3481 ggtcggtgcc gccatcgagg acctcgtggc cgccgagctg gtctacgagc ggctcgagcg
     3541 cagccgcgat cccgggtccg gccgctaggc gcatgctcgg cttcctcggc agattcggcc
     3601 gcgccgaggg cctgcggcag ctcgatgcgg cgctgcgcgc cgtcgacctc cacccggccc
     3661 tggtgcccga agccgtgaag ctcaccaccc tccgccagct ccagcaggaa gccggcggca
     3721 agccccggcc cgccgacttc gccgccgccg ccgagctcct cgcctactgc atgaccggtc
     3781 ccgaggcttt cgcgcaggcc accgacccga cgctcaccgc cgccgtcgag acccgcatcg
     3841 aagccgccat ccaggccggc gacagcctcg acgcccgcct cgtcctcctc accctccacg
     3901 ccggcctcac ccagccggcg gtggtggcaa gattcgggct ggaggccggc tgaacggtcc
     3961 ttcgtagtcg cggcaaggtg agtgttgggg gacatacagt atcttctttg gttgtcgatc
     4021 cgagcgtgtc acataggatg accaatatgg cggcgtccaa tatgcctgca aacaccccta
     4081 ccggacctcc aaaccagagc agtgtagaat ttttgattgc gtccgaatag gttttctatt
     4141 ggcttcgttt ctatcgatgt tatgatgact agaaagttat cataaagcca ctatctgaca
     4201 tgcgagtttg tcttacaatt tctccatttc tgatcaaaaa attgtaagat tttgctccag
     4261 tatatccccc aaatgaattc ttcgcattaa tcaaaactgg cacgaagtaa ccaaaagtgg
     4321 ccccgccacc ctgaatgggt gggtcttgaa aatagcccct ttgacacgcc ccaaactcgt
     4381 atctggcaga ctccggatct ttcaagtaga gccgcatgct atttttgacg acttcctcgc
     4441 attcacgctg gagaattgct cgaccgtagt ccgctgagga aagctcttgt tgcgttggcg
     4501 ccgtggcgca tgacgcgatg atgataactg caagcaaaag cgtgatgcaa ttcctcaagg
     4561 attgtgctcc tcgatttgtt aagtgcgagg ccctagcggc aaaaacgagt atcgctgcca
     4621 accgaatggt gggtcacagg ccgctgcgat ccacccattc ggtcgattga tgttgcccca
     4681 tgagcaatca actcgccaat gtacgacctg cgttccctag cgtatttttc atggcgtgtg
     4741 tgtccagtcg gtcgcaagca gcagtcattg c
//
LOCUS       DAOTFI010000310         4738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_21420_length_4738_cov_6.006833, whole genome shotgun sequence.
ACCESSION   DAOTFI010000310 DAOTFI010000000
VERSION     DAOTFI010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21420_length_4738_cov_6.006833"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1120
                     /locus_tag="P5558_21835"
                     /pseudo
     CDS             84..1120
                     /locus_tag="P5558_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028714619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1552..1731)
                     /locus_tag="P5558_21840"
     CDS             complement(1552..1731)
                     /locus_tag="P5558_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27024.1"
                     /translation="MIAGTNGLGYMSIEAVQWHQTETVILGMILIGIPWLILDRLLFV
                     PLEKATIVRWGLIQR"
     gene            complement(1772..3280)
                     /locus_tag="P5558_21845"
     CDS             complement(1772..3280)
                     /locus_tag="P5558_21845"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY27025.1"
                     /translation="MTRRPNLLFLFSDQHAQRVAGCYGDGVALTPHLDALAARGVTFD
                     NCYCPSPICVPSRMSMLTGRYPSAQHCWTNDDHLSTEAPTFAHALGAAGYRPTLAGRA
                     HFMGPDQMHGYVQRFVGDHSPNHAGLPRHDLGVLEGANDPWSVSVIRSGRGQSAYQLK
                     DQASTDAAVEHLHELGRRQRGGEDEPFALTVGWILPHAPFVATAEDYAHFDGRVGPPD
                     LAPPATGDEHAWIGWWRRNRELGERTTDEIMRARTAYYGLVMRLDRMIGEVLAALESA
                     GLADNTLVVYASDHGEQIGERGLWWKHTLYDESAKVPCVLAWPGRLPEGERRAAVTNL
                     TDLTATMLDAVGAAPLPSAQASSFLDVARDGSTPWLDATFSEYCTDTVPAWTGGMAVQ
                     QRMLRAGRFKLIYYHGYEPQLFDLEDDPREQHDLARSRRHQAVREQLLRQLLADWDPE
                     AIARRMRERRREKDVLASWAGATRPPDQHRWPMTPDMNRLDDLEPGTRGGAA"
     gene            complement(3289..4005)
                     /locus_tag="P5558_21850"
     CDS             complement(3289..4005)
                     /locus_tag="P5558_21850"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY27026.1"
                     /translation="MNEARNSAISGQSAGRDEPGDGARAPMLREQAVTAFKERLFRGD
                     LRPGQFLSQRELTLFLGLPLAPVRDAVRRLESEGLVRVIPQRGIAVAEVSLQFVRESF
                     ELRKALETWSARRFAETGPVALLDTLTDEVRTLEAEVAQDLTPELRGRVILLDRRLHE
                     TIVVSLDNRLLAEATRQTFDKIQLVRLVHPVTPDRLRRAFGEHLAILAALRRRDGEAA
                     AAAMDAHLDSAMRHAIGIVD"
     gene            complement(4093..4440)
                     /locus_tag="P5558_21855"
     CDS             complement(4093..4440)
                     /locus_tag="P5558_21855"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRY27027.1"
                     /translation="MNVPLPDFDTAEFRSRTTALQAGMRKAGIDALLLTTEPDVRYVS
                     GFLTRFRESPTRPWLLVVTAPGEPVAVVPAISAAPPCGRRSRPTRSRSPSSARTRRRR
                     RPASPRSIRPSVA"
     gene            complement(4451..>4738)
                     /locus_tag="P5558_21860"
     CDS             complement(4451..>4738)
                     /locus_tag="P5558_21860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27028.1"
                     /translation="FASLCGYLLSAFSGGASLNMGEEYLLASIAVVVIGGTSVAGGFA
                     NLPGLWGAALFLFLIVTMLNTYGLGAGIRYVLTGLIIIAVITAAGGRKPLR"
BASE COUNT          681 a         1478 c         1882 g          697 t
ORIGIN      
        1 ggaggtgatg gggtggacgc ccctcgacgg catctgttgt gccagagtgg cggtgtggtt
       61 gaccgccagc agaggaggcg tccgtggtcg agattagcat gatcggggtg gacctggcga
      121 agcacgtttt tcagctgcac ggggcgcggc tgacgggcgg gtggtgttcc ggcggcgggc
      181 gcggcgggag cagctgctgg cgttcttcgc ggcgcagccg gcttgtgacg tggtgatgga
      241 ggcttgcggg agcgcgcatt actgggcgcg ggagatcggc gggctggggc atcgggtgcg
      301 gctggtggcg ccggcttacg tcaagccgtt cgtcaagcgg cagaagaatg acgcggcgga
      361 tgccgaggcc attgtcgagg cggcgcagcg gccgacgatg cggttcgtgg cggtgaagag
      421 cgccgagcgg caggccgcgg cgatggtgtt caagacgcgg gacttgctgg tgcggcagcg
      481 gacgagcctg gtgaatgcgt tgcgggcgca tctggcggag tttggcctgg tggcacccaa
      541 gggcatttgc catgtcggcc ggctcgaggc gctgctggcg gaggatacga cgctgcccga
      601 gccggtgcga cggctgtgcg gcgagctggt ggcgttgatc gccggcctga cggcgcggct
      661 cgacatgctc gaccgggagg tcagggagca cgcgcggacg gacgagctgg cccggcgcct
      721 gcagaagatt ccggggatcg ggccggtcac cgccctggcg cttgcggtgc tcgcaccgcc
      781 gcctgagacc ttcaaacggg gccgcgatct cgccgcctgg gtcgggctca cgccccgaca
      841 gcattcgagt ggcggggtca gtcggctcgg caagacctcg gagatgggcc agcgcgatct
      901 gcggcggctc ttgatcatcg gcgccagcgc ggtcgttcgc tgggcgaggc ggccgcatct
      961 gcaggatcga ttggcgccga agggctcctg gctggctggc atgctcgcgc gcaagccgcc
     1021 catgctggtg gtcgtggcgc tggccaacaa gacgacacgt gtcgcctggg cgctgatggc
     1081 gcacggcgac gcgtatcgag ctcccgcacc ggctggttga tcaaccggcg gtcggcgagg
     1141 ctgtcgcgga tgggaggagg gcgaagctca cgtcatgggc agacggtcga acgagacggg
     1201 atcgggaaaa ccagtccttg gggacaggcg cttcgcagcg cgcggaggtg acttggactc
     1261 gatccggcgg atctccatac gggcccgcgg catgtcgaac gccgcaacca gaggccggac
     1321 acatgtcagc acccgacccg acgtccagac gaaggccaag ttctcgcttg catccaaagg
     1381 ggcgtccaca catgtcaccc ttgggagggg cgccatcgcg cgcccctccc tgagcgtccg
     1441 cccgccgagt cggagacgag gagggcgagg cgatccgcgc gcagcgtgct gaagcacgtg
     1501 agcacggatc gaccgaagcc cgacgaagta tccggccggc cggcggcgcg ctcagcgctg
     1561 gatgaggccc cagcggacga tggtggcctt ctcgaggggg acgaagagga ggcggtcgag
     1621 gatgagccag gggatgccga tgaggatcat gccgaggatg accgtctcgg tctggtgcca
     1681 ctggacggcc tcgatgctca tgtagccgag gccgttggtg ccggcgatca tctggccggc
     1741 ggcgccgcga gccgggctcg cgcgcgccgc ttcacgccgc gccgcccctg gttcccggct
     1801 cgagatcgtc gagccggttc atgtcgggcg tcatcggcca gcgatgctgg tcgggtggtc
     1861 gcgtggcgcc ggcccagctt gccagcacgt ccttctcgcg gcgccgctcg cgcatccgcc
     1921 gggcgatggc ctcggggtcc cagtcggcga ggagctggcg caagagctgc tccctgaccg
     1981 cctggtggcg gcggcttcgg gcgaggtcgt gctgctccct ggggtcgtcc tcgaggtcga
     2041 agagctgcgg ctcgtagccg tgatagtaga tcagcttgaa gcggccggcg cgcagcatgc
     2101 gctgctggac ggccatgccg ccggtccagg cgggcacggt gtcggtgcag tattcggaga
     2161 aggtggcatc cagccagggc gtcgagccgt cccgggccac gtcgaggaag ctggaggcct
     2221 gggcgctcgg caggggggcg gcgccgacgg cgtccagcat ggtggcggtg aggtcggtga
     2281 ggttggtgac ggcggcgcgg cgctcgccct cgggcagcct gcccggccag gccaggacgc
     2341 agggcacctt cgccgattcg tcgtagagcg tgtgcttcca ccagaggccg cgctcgccga
     2401 tctgctcgcc gtggtcgctg gcatagacga cgagcgtgtt gtcggcgagg ccggccgatt
     2461 cgagggcggc cagcacctcg ccgatcatcc ggtcgagccg catgacgagg ccgtaatagg
     2521 cggtccgcgc ccgcatgatc tcgtcggtgg tccgctcgcc cagctcgcgg ttccgccgcc
     2581 accagccgat ccaggcgtgc tcgtcgcccg tggcgggcgg ggcgaggtcg ggcgggccga
     2641 cccggccgtc gaaatgggcg tagtcctcgg ccgtggcgac gaagggggcg tgcggcagga
     2701 tccagccgac cgtgagggcg aagggctcgt cctcgccacc gcgctggcgg cggccgagct
     2761 cgtgcaggtg ctcgaccgcg gcatcggtcg aggcctggtc cttgagctgg taggccgact
     2821 ggccgcggcc ggagcggatg acgctgacac tccaggggtc gttcgcccct tcgagcacgc
     2881 cgaggtcgtg gcgcggcagg ccggcatggt tcgggctgtg gtcgccgacg aagcgctgga
     2941 catagccgtg catctggtcc gggcccatga aatgcgcgcg gccggcgagg gtcggccggt
     3001 agccggcggc acccagcgca tgggcgaagg tcggcgcctc ggtcgagaga tggtcgtcgt
     3061 tggtccagca gtgttgcgcc gaggggtagc ggccggtcag catcgacatg cgcgacggca
     3121 cgcagatcgg cgaggggcag tagcagttgt cgaaggtgac gccgcgggcg gccagcgcgt
     3181 cgagatgggg ggtgagcgcc acgccgtcgc catagcagcc ggcgacccgc tgggcgtgct
     3241 ggtcggagaa gaggaagagc aggttgggtc gcctggtcat gggttccttc aatcgacgat
     3301 gccgatggcg tggcgcatgg cgctgtcgag atgggcgtcc atcgccgccg ccgccgcctc
     3361 gccgtcgcgc cggcgaaggg ctgccagaat ggcgagatgc tcgccgaagg cgcggcgcag
     3421 gcggtcgggg gtgacgggat ggaccaggcg cacgagctgg atcttgtcga aggtctggcg
     3481 ggtggcttcg gccaggagcc ggttatcgag cgagaccacg atggtctcgt gcagccggcg
     3541 atcgagcagg atcacccggc cgcgcagctc cggcgtgagg tcctgggcga cctcggcctc
     3601 gagtgtgcgc acctcgtcgg tcaatgtgtc gaggagcgcc accggcccgg tctcggcgaa
     3661 ccgcctggcg gaccaggtct ccagggcctt cctcagctcg aacgactccc ggacgaactg
     3721 caggctcacc tcggcgacgg cgatgccgcg ctgcgggatg acgcgcacga ggccctcgct
     3781 ctcgagccgg cggacggcgt cgcggaccgg cgcgagcggc aggccgagga agagcgtcag
     3841 ctcgcgctgg ctgaggaact ggccgggcct gagatcgccg cggaagagcc gctccttgaa
     3901 ggcggtcacc gcctgctcgc gcagcatggg cgcacgcgca ccgtcgcccg gctcgtcgcg
     3961 gccagccgat tggcccgaaa tggcgctgtt ccgcgcttcg ttcacgcgct gttccctgat
     4021 cttgcgctga aatgttaggc gggtgccagt tctgctgtca agggcgggcg gcccgctgtc
     4081 ggccgagcgc catcaagcga ccgaaggccg gatcgagcgg ggcgatgcgg gcctccttct
     4141 tctcctcgta cgagccgaag atggcgatct cgagcgcgtc ggccgtgagc gccgcccgca
     4201 gggcggggcg gcgctgatcg ccgggaccac ggcgacgggc tcgcccgggg ccgtgaccac
     4261 gaggagccag ggccgggtcg ggctttcccg gaaccgggtg aggaagccgc tgacatagcg
     4321 gacatccggc tcggtggtca gcagcagggc gtcgatgccg gccttgcgca tgcccgcctg
     4381 cagtgcggtc gtccggctcc ggaactctgc cgtgtcgaag tcggggagcg gcacgttcat
     4441 ggccagttcc tcagcgcagc ggcttgcggc cgcctgcggc ggtgatgacg gcgatgatga
     4501 tgaggccggt gaggacgtag cggatgccgg cgccgaggcc gtaggtgttg agcatggtga
     4561 cgatgaggaa gaggaagagg gcggcacccc acaggcccgg caggttggcg aagccgccgg
     4621 cgaccgaggt gccgccgatc acgacgacgg cgatcgaggc gagcaggtac tcctcgccca
     4681 tgttgagcga ggcgccgccc gagaaggccg agaggagata gccgcagagc gaggcgaa
//
LOCUS       DAOTFI010000311         4687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_21737_length_4687_cov_6.539292, whole genome shotgun sequence.
ACCESSION   DAOTFI010000311 DAOTFI010000000
VERSION     DAOTFI010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21737_length_4687_cov_6.539292"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="P5558_21865"
     CDS             complement(<1..1765)
                     /locus_tag="P5558_21865"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRY27029.1"
                     /translation="MAPRPEHFFNRELSWLAFNERVLEEASNVAHPLLERLRFLSISS
                     SNLDEFYMVRVAGLKGLAHAGVTTQSQDGLTPARQLEAINERAGRLMADQQRIWHGLA
                     TELREQGISVLELDEVSEADRADLRKRFADEALAVLTPIAIDPAHPFPFIPNCGMGVV
                     LQLSQGRDEDLIGLLLLPGKLDRFVRLPGPEARYVRLESLIAHWTDLLFPGFGVKSFG
                     YFRVIRDSDIEIEEEAEDLVRLFETLLKRRRRGHVIRLTTDVRMPRALRVFLMRQLNV
                     EAADVFELEGILGLSDTRQLIGSDRPDLVFKPYSPRYPERIKDFGGDCFAAIRQKDFV
                     VHHPYESFDVVVQFLNQAARDPDVIAIKQTLYRTSDGSPVVAALCDAADRGKSVTALV
                     ELKARFDEERNIQWARDLERAGVQVVYGFIELKTHAKVSMVVRREGGGLRTYCHFGTG
                     NYHPITAKVYTDLSFFTCDPALGRDAARTFNYMTGYAEPQGLEKLAIAPVNLRQKLIE
                     LIDAEAEHARAGRKAAIWAKMNSVVDEEVIEQLYAASQAGVKIDLVVRGICCLRPGVP
                     GLSENIRVKSIIGRFLEHSRII"
     gene            1969..2487
                     /locus_tag="P5558_21870"
     CDS             1969..2487
                     /locus_tag="P5558_21870"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY27030.1"
                     /translation="MLEMLVLRHAKSRWGEPGTTDHDRTLAPRGVEAAKRMGRLIRER
                     DWLPDRILCSTAARASATLALAMATWPERAAPAVEHTSRLYMASPQQLLEVVRAQAAG
                     CKRLLLVGHNPGMGAFVLRLAGAGEPAALAAVEEKFPTAALARLGLDLEQWRNAEWGS
                     ARLLDFIRPKDL"
     gene            complement(2499..4349)
                     /gene="mdoH"
                     /locus_tag="P5558_21875"
     CDS             complement(2499..4349)
                     /gene="mdoH"
                     /locus_tag="P5558_21875"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003962.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRY27031.1"
                     /translation="MFTPNSTGREQFWLRRTIFAALVLTTAASAIGAFAWLVGRDGLD
                     VLDWLMLVFFALTLPWVAIGFWCAVLGLLILHLKKDPVAYVTPMARDGDGDRPLEGRT
                     ALLMAIHDEDPEAVFRHLRATVESVDRAGAGALVDIHVLSDTRDAAIAAHELRLFESW
                     RNTEARSDRLHYRRRKANTDHKTGNIWEWLDRAGGTYDYFVTFDADSLMSGRLLARLV
                     RIMDRNPDLGILQTLIVGLPSSSAFARIFQFGMRHGMRSYTTGSAWWMGDAGPYWGHN
                     AIIRTAAFMQHCRLPHLPGKPPLGGLILSHDQVEAALMRRAGYQVRVLPEEGGSFEVN
                     PPTLPEFIRRDLRWCQGNMQYWQLLPVAGFRAMGRLQLVLAILMYMAAPAWLFFMLAG
                     FAKAMLIGFGPGESGELASQWTPTIAAMGIGLFAIMMTVNLTPKLAGLIDVALRPEAR
                     RAYGGGHMLAASAIVELAASLVLGPAMAVAQTIFIAGLPFGRMVRWEAQVRGDHSVSL
                     ADAARGLWPQLLLGLMLLGGFALAMPAVLPWTLPIIGGLVLAIPFAWLTARPGLGRLL
                     TRARICAIPEERDAPLTLQLAGYLPRADGTGRVRRADVDPRLVDASIERA"
BASE COUNT          762 a         1519 c         1706 g          700 t
ORIGIN      
        1 agatgatccg tgaatgctcg aggaagcggc cgatgatgct cttgacgcga atgttctccg
       61 agaggcccgg cacgcccggc cgcaggcagc agatgccgcg caccacgagg tcgatcttca
      121 cgccggcctg gctcgccgca tagagctgct cgatgacctc ctcgtcgacc accgagttca
      181 tcttcgccca gatcgccgcc ttgcggccgg cgcgggcatg ctcggcctcg gcatcgatca
      241 gctcgatcag cttctggcgc aggttgaccg gggcgatcgc cagcttctcc agcccctgcg
      301 gctcggcata gccggtcatg tagttgaagg tgcgcgccgc atcgcgcccc agcgccgggt
      361 cgcaggtgaa gaaggagagg tcggtgtaca ccttggcggt gatggggtgg tagttgccgg
      421 tgccgaaatg gcagtaggtg cgaagcccgc cgccctcgcg gcgcaccacc atcgagacct
      481 tggcgtgcgt cttgagctcg atgaagccat agacgacctg cacgccggcc cgctccagat
      541 cgcgcgccca ctggatgttg cgctcctcgt cgaagcgcgc cttgagctcg accagggcgg
      601 tcaccgactt gccgcgatcc gccgcatcgc agagtgccgc caccaccggc gagccgtccg
      661 aggtccgata gagcgtctgc ttgatggcga tgacgtccgg gtcgcgggcc gcctggttga
      721 ggaactggac gacgacgtcg aagctctcgt agggatggtg gacgacgaag tccttctggc
      781 ggatcgcggc gaagcagtcg ccgccgaaat ccttgatgcg ctcgggatag cgcgggctgt
      841 agggcttgaa gacgaggtcc gggcggtcgc tgccgatgag ctgcctggtg tcggacaggc
      901 cgagaatgcc ctccagctcg aacacgtcgg cggcctcgac attgagctgg cgcatcagga
      961 acacgcgcag cgcccgcggc atccgaacgt cggtcgtcag gcggatgaca tggccgcggc
     1021 ggcgccgttt gagcagcgtc tcgaagaggc gcacgaggtc ctcggcctcc tcctcgatct
     1081 cgatgtcgct gtcgcgaatg acgcggaaat agccgaagct cttcacgccg aagcctggaa
     1141 acagcaggtc ggtccagtgg gcgatcagcg attcgaggcg gacatagcgc gcctcgggcc
     1201 ccggcaggcg gacgaagcgg tcgagcttgc ccggcaggag caggagcccg atcaggtcct
     1261 cgtcgcggcc ctggctgagc tgcaggacga cgcccatgcc gcaattgggg atgaagggga
     1321 aggggtgggc cggatcgatg gcgatcggcg tgagcacggc cagcgcctcg tcggcgaagc
     1381 gcttgcgcag gtcggcgcga tcggcctcgc tcacctcgtc gagctcgagc acgctgatgc
     1441 cctgctcacg cagctcggtc gcgagaccat gccagatgcg ctgctggtcg gccatcagcc
     1501 gccccgcgcg ctcgttgatc gcctcgagct ggcgggccgg tgtcaggccg tcctggctct
     1561 gggtggtcac gccggcgtgg gcgaggccct tgaggccggc cacgcggacc atgtagaact
     1621 catcgaggtt gctcgacgag atcgacagga agcgcaagcg ctcgaggagg ggatgggcaa
     1681 cgttggacgc ctcctcgagc acccgttcat tgaaggcgag ccaggagagc tcacggttga
     1741 agaaatgctc gggccgcggg gccatgggtt cggcgacggt cgctgcggga gcccggccgc
     1801 gctcggctac cggcgatacg ggctcgacgc tggaggtcgg ggccatctgc tcgttggtca
     1861 tcgacttcgt cgctcgtcaa atggttcatg gaacagtgct gtcatgtgat cgccaagcta
     1921 caggctcgct gcggtcgtgt catgccagga cggtcggata gggtcacgat gctggaaatg
     1981 ctggttctgc ggcacgccaa atcgcgttgg ggcgagccgg gcacgaccga ccacgatcgc
     2041 acgctcgccc cgcgcggcgt cgaggcggcg aagcggatgg gccggctgat ccgggagcgg
     2101 gactggctcc ccgaccggat cctctgctcg accgcggcac gcgcgtcggc gacgctcgcg
     2161 ctcgccatgg ccacgtggcc cgagcgcgcg gcgccggctg tcgagcatac gagccggctc
     2221 tacatggcga gcccgcagca actgctcgaa gtggtgcggg cgcaggcggc gggctgcaag
     2281 cgcctgctgc tggtcggcca caatccgggg atgggtgcct tcgtcctgcg cctggccggc
     2341 gccggcgaac cggccgccct cgccgccgtc gaggagaagt tcccgaccgc cgcgctcgcc
     2401 cggctgggcc tcgacctgga gcaatggcgg aacgccgaat ggggcagtgc gaggctgctc
     2461 gacttcattc ggccgaagga tctctagccg gctgtcgatc aggcccgctc gatcgaggcg
     2521 tcgaccagcc tcgggtcgac gtcggcccgg cgcacgcgcc cggtgccgtc ggcgcgcggc
     2581 agatagccgg ccagttgcag ggtgagcggt gcatcccgct cctccgggat cgcgcagatt
     2641 cgggcccggg tgagcaggcg gccgaggccg ggccgggcgg tgagccaggc gaagggaatg
     2701 gcgagaacca ggccgccgat gatcggcagg gtccagggca ggaccgccgg catggccagg
     2761 gcgaagcccc ccagcagcat caggccgagc aggagctgtg gccagaggcc gcgcgccgcg
     2821 tcggcgaggg agacgctgtg gtcgccgcgc acctgcgctt cccagcggac catgcggccg
     2881 aagggcagcc cggcgatgaa gatcgtctgc gccaccgcca tggccggccc gagaacgagc
     2941 gaggcggcga gctcgacgat ggccgaggcg gccagcatgt ggccgccgcc ataggcacgg
     3001 cgggcttccg ggcgcagcgc cacgtcgatg aggccggcca gcttcggggt caggttgacg
     3061 gtcatcatga tggcgaagag gccgatgccc atggcggcaa tggtcggcgt ccattggctg
     3121 gccagctcgc cggattcgcc cgggccgaag ccgatcagca tcgccttggc gaagccggcg
     3181 agcatgaaga agagccaggc gggcgcggcc atgtacatga ggatcgccag cacgagctgg
     3241 agccgcccca tcgcccggaa gccggccacc ggcaggagct gccagtactg catgttgccc
     3301 tggcaccagc gcaggtcgcg gcggatgaac tcgggcaggg tgggcgggtt gacctcgaac
     3361 gagccgccct cctcgggcag gacccggacc tggtagccgg cacggcgcat cagcgccgcc
     3421 tcgacctggt cgtggctgag gatgaggccg ccgaggggcg gcttgcccgg cagatgcggc
     3481 agccggcaat gctgcatgaa ggcggccgtg cggatgatgg cgttgtggcc ccaatagggg
     3541 ccggcatcgc ccatccacca ggccgagccg gtggtgtagg agcgcatgcc gtggcgcatg
     3601 ccgaactgga agatgcgggc gaaggcggag gaggagggga ggccgacgat cagggtctgg
     3661 aggatgccga gatccggatt gcggtccatg atcctgacga gccgtgcgag cagccgtccg
     3721 ctcatcaggc tgtcggcgtc gaacgtcacg aaatagtcgt aagtcccgcc agcacggtcg
     3781 agccattccc agatattgcc ggtcttgtgg tcggtgttcg ccttgcgccg gcgatagtgc
     3841 aggcgatccg atcgggcttc cgtgttgcgc cacgattcga agagccgcag ctcatgggcc
     3901 gcgatcgccg catcgcgcgt gtcgctcagc acgtggatgt cgacaagggc gcccgcgccg
     3961 gcccgatcca cactctcgac ggtggcgcgc aggtggcgaa agacggcctc gggatcctcg
     4021 tcgtggatcg ccatgaggag cgccgttcgg ccctcgagcg gccggtcgcc gtcgccgtcg
     4081 cgagccatcg gcgtgacgta ggcaaccgga tccttcttca ggtggaggat cagcaggccg
     4141 agcacggcgc accagaagcc gatggcgacc cagggcaggg tcagggcgaa gaagaccagc
     4201 atcagccagt cgaggacatc gaggccatcc cggccgacca gccaggcgaa ggctccgatg
     4261 gcgctggccg cggtggtcag cacgagtgcg gcgaaaatgg tgcggcgcag ccagaactgc
     4321 tcgcggcccg tcgagttcgg cgtgaacatc ccttatgtca ccgttcttct ataaattcag
     4381 cgtaccgtag aggcgttccc cggcgtgagc cgcaatgcac ccatcgccgg tctccctgat
     4441 atagaaacac cgaccctcga ggcacatacg aattgtaaaa atcgcgcgag ccggggtcgg
     4501 aggtggagtc gggcggtcag gcgatctccc gcttcttgcc gatccggggc aggcgccgcg
     4561 ccagatactc gccggtgtgg ctgccatccg ttgccgccac ctgctccggc gtgccctcgg
     4621 cgatgagccg gccgccgccg gtgccgccgt ccgggcccat gtcgaggatc cagtcggcgg
     4681 tcttgat
//
LOCUS       DAOTFI010000312         4661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_21892_length_4661_cov_7.377985, whole genome shotgun sequence.
ACCESSION   DAOTFI010000312 DAOTFI010000000
VERSION     DAOTFI010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21892_length_4661_cov_7.377985"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="P5558_21880"
     CDS             complement(<1..895)
                     /locus_tag="P5558_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27032.1"
                     /translation="MQRQLLITVLAAAASAALYLSLTTGVLGTPLFAYFVQLPVLFVG
                     LSFGLAPAGLAGFGSAGLVMAFGGGIAALVFLVVQVAPCLITTRQALLSRVGKDGKTE
                     WYPLGLVLCQLVLYAAVVMVLALFWVRWSTGSIEASFTAALTEVLGQFAGEAGELAAV
                     AGMVADYAAIVPGIIAVSWVLMTVINAALAQLLALRGGQAQRPGAPLAELWLPGWCGP
                     ALAIGTLAALVAGGDLAFFAQGLAALLAVPFLMQGLAVVHSLAHRLPARGLALAGFYL
                     VLILFSWPLVVVVVVLGLVEDW"
     gene            complement(921..1151)
                     /gene="rpsR"
                     /locus_tag="P5558_21885"
     CDS             complement(921..1151)
                     /gene="rpsR"
                     /locus_tag="P5558_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRY27033.1"
                     /translation="MTIRRPFFRRKKASPFAHADASPIDYKDVKLLQRFVSERGKIVP
                     RRITAVTAKEQRQLAKAIKRARELALLPYVAG"
     gene            complement(1289..1645)
                     /gene="rpsF"
                     /locus_tag="P5558_21890"
     CDS             complement(1289..1645)
                     /gene="rpsF"
                     /locus_tag="P5558_21890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRY27034.1"
                     /translation="MPLYEHTFIARPDLSSQQAQTLADELGAIVSANGGEVKKVEYWG
                     LRSLAYRLKKHRKAHYVHLNLDASPSAVAELERNERIHDDVVRYLTVKIDTVDPEPSI
                     VMQVKSSRDERRRDRP"
     gene            2099..2332
                     /locus_tag="P5558_21895"
     CDS             2099..2332
                     /locus_tag="P5558_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27035.1"
                     /translation="MFLAAGWRYEDNAPENLARLRRFALDLLRAGQGEGSTPGKIERA
                     TWDDASLIKILSSARCDRPAVEALALARAGGLR"
     gene            2383..3753
                     /locus_tag="P5558_21900"
     CDS             2383..3753
                     /locus_tag="P5558_21900"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRY27036.1"
                     /translation="MPGKLDLEGLAERVSLGLIDTVLVCFTDLQGRLIGKRFHAQHFV
                     ASGHAETHACDYLLANDIEMEPVPGYAAASWQRGYGDFVMAPDLATLMEVPWLEGTAL
                     VLCDVLDHHDHAPLAHSPRAILKGQVARLEERGMRAYLASELEFYLFEETYKSARAKG
                     WRGLQTANDYIQDYVISATSKEERVMRAIRNHLHDAGIPIENSKGEWGPGQEELNVRY
                     ADALTMADRHVVIKNGIKEIAHGLDAAVSFMAKYDNALAGSSCHVHASLWDADGARPL
                     FLDEAAEDGMSPLMRQFVGGMLRYAPDYTWFLAPYINSYKRFQAGTFAPTRMVLSGDN
                     RTAGFRLVGERTKAQRIECRIGGADLNPYLAFAALIAAGLAGIDEGLDCGEPYAGDAY
                     GDDDLPEVPKTLRAAAEALDNSAMLRTALGDAVVDHYVHTARWEQAEYDRRVTDFDLS
                     RGFERY"
     gene            3762..>4661
                     /locus_tag="P5558_21905"
     CDS             3762..>4661
                     /locus_tag="P5558_21905"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY27037.1"
                     /translation="MSDKVQIVSPIDDSVVAQRPVARDSEIEDAIATARGSQRAWARV
                     SVGERARICLAMLDSMLAMQEDLTVELARMMGRPVRYGGEMGGFAERTRHMAAIAREA
                     LAPLTIEDTDQSRRLIAREPLGVVLVIAPWNYPYLTAVNTIVPALIAGNAVILKAASQ
                     TLLTGERFQRAFEEAGMQESVFEHLVMSHAQTEKLIASRAVDHVTFTGSVAGGRAIER
                     AAAGSFIGVGLELGGKDPAYVRADADLDHAIENLVDGAFFNSGQSCCGIERIYVAEEL
                     FDRFVDGFAATTGSYVLDDPRDEA"
BASE COUNT          760 a         1568 c         1658 g          675 t
ORIGIN      
        1 cccagtcctc gacgagaccg aggaccacga cgaccacgac gagcggccag ctgaacagga
       61 tgaggacgag gtagaagccg gcgagcgcca gcccgcgggc gggcagccga tgggcgaggc
      121 tatggacgac ggcgaggccc tgcatcagga agggcaccgc cagcagcgct gccagaccct
      181 gggcgaagaa ggcgagatcg cccccggcga ccagggccgc gagcgtgccg atcgccagcg
      241 ccggcccgca ccagcccggc agccagagct cggcgagcgg tgctcccggc cgctgcgcct
      301 gcccgccgcg cagcgccagg agctgggcga gcgcggcgtt gatcaccgtc atcagcaccc
      361 aggagacggc gatgatgccg ggcacgatgg ccgcatagtc agcgaccatg ccggcgaccg
      421 cggcgagctc gcccgcctcg ccggcgaact ggccgagcac ctcggtcagc gccgccgtga
      481 aggacgcctc gatgctgccc gtgctccagc gcacccagaa gagggcgagg accatgacga
      541 ccgccgcgta gaggacgagc tggcagagca cgaggccgag cgggtaccac tcggtcttgc
      601 cgtccttgcc cacccgcgag agcagcgcct ggcgggtggt gatcaggcag ggcgccacct
      661 ggaccacgag gaagacgaga gcggcgatgc cgccgccgaa cgccatgacg agcccggccg
      721 agccgaagcc ggcgaggccc gccggcgcca gcccgaacga caggccgacg aagagcaccg
      781 ggagctggac gaaataggcg aagagcggcg tgcccaggac gcccgtggtc agcgacaggt
      841 agagcgctgc gctggccgcg gccgcgagga cggtgatgag gagctgccgc tgcacggatg
      901 ctcgtccagt atcggtcggc tcagccggcg acgtagggca ggagcgccag ctcgcgggcc
      961 cgcttgatcg ccttggccag ctgacgctgc tccttggcgg tcacggcggt gatccggcgc
     1021 gggacgatct tgccacgctc cgagacgaag cgctgcagca gcttcacgtc cttgtagtcg
     1081 atcggcgatg cgtcggcgtg ggcgaaaggg ctcgccttct tgcggcggaa gaagggtcgg
     1141 cggatggtga tgttcggcgg ctcgccgccg cggctgccac ggtccgggcg gtcgccacgc
     1201 tcgcgatcgc cgtaggagcc ctgcgggcgg tctctggttt ccatgatcta acggttcctc
     1261 gtctcgactt tgcgattggc ggaagagctc aggggcggtc gcggcgccgc tcgtcgcggc
     1321 tggacttgac ctgcatcacg atcgacggct cgggatcgac cgtgtcgatc ttgaccgtca
     1381 ggtagcggac cacgtcgtcg tggatgcgct cgttgcgctc gagctcggcg accgccgaag
     1441 gcgatgcgtc gaggttgagg tgcacgtagt gagccttccg atgcttcttc agccggtagg
     1501 cgagggagcg gagcccccag tactcgacct tcttcacttc gccgccattc gccgagacga
     1561 tggcgccgag ctcgtcggcc agggtttgtg cctgctgcga cgagaggtcg gggcgggcga
     1621 tgaacgtatg ctcgtagagc ggcatctttg ccgttgctcc tctggatttg gacgacaaca
     1681 gggaccgccg cgtcatcttc acgcgacgat ccaccgtcac tacagattcg ctgccgtcgg
     1741 ccggtacgcc gacccaccgc gaggatggcg cggttatacg cggtttggcc gcggtcgcaa
     1801 gcgtgttcgg tcaggggttg cccgacgctc ctgctcggga tgcgctggga gacggggctt
     1861 ccgccgtgag cgctgcggtt gcgagagctg cacgcggatg aagtgcccca agcaaatcca
     1921 atgcaatcgc tgcaatgcgg cagccgacgg cttggctgat gaagcagaag ccgatgtcgg
     1981 tcatggcgga gcgggaaggg ggcaagcccg agtgggcggg ccgggccgag atcgacgacg
     2041 cccccgccgt ggcaatgcgc aacggcgcga tcggcggtgc cgaggagccg ctgctgcgat
     2101 gtttcttgcg gctggatggc ggtatgagga caacgccccg gagaacctcg cccgcctgcg
     2161 ccgcttcgcg ctcgatctcc tgcgcgccgg ccagggcgaa ggatcgaccc ccggcaagat
     2221 cgagcgggcc acgtgggacg acgcttccct catcaagatc ctgtcatccg cccgatgcga
     2281 tcgccctgcg gtcgaagcgc tcgcactggc gcgagccggc ggcttgcgct aaccacgaag
     2341 gccagcagcg ggcggtgaca gggcccatat cggggaggtt tcatgccggg caagctcgat
     2401 ctcgaaggtc tcgccgagcg ggtcagcctc gggctgatcg acacggtgct cgtctgcttc
     2461 accgacctgc agggccggct catcggcaag cgcttccatg cccagcattt cgtcgcgtcc
     2521 ggccacgccg agacgcacgc ttgtgactac ctgctcgcca acgacatcga gatggagccg
     2581 gtgccgggct acgcggcggc gagctggcag cgcggctatg gcgatttcgt catggccccg
     2641 gatctcgcga ccctgatgga ggtgccgtgg ctcgagggca cggccctcgt gctctgcgac
     2701 gtgctcgacc accacgatca cgcacccctg gcgcacagcc cgagggccat cctcaagggc
     2761 caggtcgcac ggctcgagga gcgcggcatg cgggcctatc tcgcctccga gctcgagttc
     2821 tacctcttcg aggagaccta caagagcgcg cgggccaagg gctggcgcgg actccagacc
     2881 gcgaacgact acatccagga ctatgtgatc agcgccacga gcaaggagga gcgggtgatg
     2941 cgggcgatcc gcaaccacct gcacgatgcc ggcatcccga tcgagaacag caagggcgaa
     3001 tgggggccgg ggcaggagga gctcaacgtc cgctacgctg acgctctcac catggccgac
     3061 cggcacgtgg tcatcaagaa cggcatcaag gagatcgcgc acgggctcga cgccgcggtc
     3121 agcttcatgg ccaagtacga caacgcgctc gccggctcgt cctgccacgt ccacgcctcg
     3181 ctctgggacg ccgatggggc gcggccgctc ttcctcgacg aggcggccga ggacggcatg
     3241 tcgccgctga tgcgccagtt cgtcggcggc atgctgcgct acgcaccgga ctacacctgg
     3301 tttctcgccc cctacatcaa ctcctacaaa cgcttccagg ccggcacctt cgcgccgacc
     3361 cggatggtgc tctcgggcga caaccgcacc gcgggcttcc gcctcgtcgg cgagcggacg
     3421 aaggcgcagc ggatcgagtg ccggatcggc ggtgcggacc tcaaccccta tctcgccttc
     3481 gccgccctca tcgcggccgg tctcgccggc atcgacgagg ggctggattg cggcgagccg
     3541 tacgccggcg acgcctatgg cgacgacgac ctgccggagg tgccgaagac gctgcgggcc
     3601 gccgccgagg ccctcgacaa ctccgccatg ctgcgcacgg cgctgggtga tgcggtggtc
     3661 gaccactatg tccacaccgc ccgctgggag caggccgagt acgaccggcg ggtgaccgat
     3721 ttcgatcttt cgcgcggttt cgagcgctac tgagagggct catgtccgac aaggtgcaga
     3781 tcgtctcgcc gatcgacgac agcgtcgtcg cccagcggcc ggtggccagg gacagcgaga
     3841 tcgaggacgc gatcgccacg gcgcgaggct cccagcgcgc ctgggcgcgc gtctccgtcg
     3901 gggagcgggc gcggatctgc ctcgccatgc tggattccat gctggcgatg caggaggacc
     3961 tcacggtcga gctcgcccgg atgatggggc ggccggtgcg ctatggcggg gagatgggcg
     4021 gcttcgccga gcgcacccgg cacatggcgg cgatcgccag ggaggcgctg gcgccgctca
     4081 ccatcgagga cacggaccag agccggcgcc tgatcgcgcg cgagccgctc ggtgtcgtgc
     4141 tggtcatcgc cccctggaac tacccctacc tgacggcggt gaacaccatc gtgccagcac
     4201 tcatcgccgg caatgcggtg atcctgaagg cggcatccca gacgctcctc accggcgagc
     4261 gcttccagcg ggccttcgag gaggccggca tgcaggagag cgtgttcgag catctggtga
     4321 tgagccatgc gcagaccgag aagctgatcg cgagccgtgc ggtcgaccat gtcaccttca
     4381 cgggctctgt cgccggcggc cgggcgatcg agcgcgcggc ggccggcagc ttcatcggcg
     4441 tcggcctcga gctcggcggc aaggacccgg cctatgtccg ggccgacgcc gatctcgacc
     4501 atgcgatcga gaacctggtc gacggggcct tcttcaattc cggccagagc tgctgcggca
     4561 tcgagcggat ctatgtggcc gaggagctgt tcgaccggtt cgtcgacggg ttcgcggcga
     4621 ccaccggcag ctacgtcctc gacgatccgc gggacgaggc g
//
LOCUS       DAOTFI010000313         4642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_22013_length_4642_cov_7.297798, whole genome shotgun sequence.
ACCESSION   DAOTFI010000313 DAOTFI010000000
VERSION     DAOTFI010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22013_length_4642_cov_7.297798"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="P5558_21910"
     CDS             complement(<1..277)
                     /locus_tag="P5558_21910"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRY27038.1"
                     /translation="MGYSIEHIEGIGGAYGAKLRDAGIASTSALLKAAADPRGRQLLA
                     QKTGIDASRILLWANMADLMRIKGIGKQFAELLEAAGVDTVKELKTRN"
     gene            448..1257
                     /locus_tag="P5558_21915"
     CDS             448..1257
                     /locus_tag="P5558_21915"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY27039.1"
                     /translation="MRVLMTGAAGGVATMLRPLLVGLYDEVVLSDRRAPEGLAAHERF
                     VAADLGDPDSLAAACDGVDGIIHLGGQAYEAEWEVIHSANITGCWNLFEAARQAGVER
                     VVFASSNHAIGFYPRRDRIGVDEPVRPDSRYGISKAFGEAVGSFYADKFGLRVLSIRI
                     GNVGPVPLDKRRLSIWIHQEDLLSLIRIGLEHPELHNAIVYGVSQNERSWWDNRTAYR
                     LGYAPRHRAEDHVDAALAAQAKLPPDPIGDLFQGGTFCAAEFAGDLKRTSS"
     gene            1447..2421
                     /locus_tag="P5558_21920"
     CDS             1447..2421
                     /locus_tag="P5558_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27040.1"
                     /translation="MTANDAVPPIRLDRYLALLALLLAIVLAFVVLGERQKAAIRPTP
                     VTGALPPPPDPRLAALAGERDRLLTEQARLADGLATAEHALAAAEADIERLKAQVDKL
                     ERDLSAEVEARAVLERRLAEVAAERDRLATEVQALTRIRESLEAELAALAAATPIPPA
                     PPSAARQEPPAAPAPEPTPAPAPAVADLLPGPDIAPEAPGPAVHGPIPPAGESATGDR
                     PGRLVILDGRRTGVADGIKAYQAGEYDAAARIWGKAAAEGEARAQFHLGSLLFEGRTG
                     PPDLVMAYVWLSRAVTGGHLPAIEVRRRVRAAMTEAQYEAALAILEAG"
     gene            2494..3873
                     /locus_tag="P5558_21925"
     CDS             2494..3873
                     /locus_tag="P5558_21925"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRY27041.1"
                     /translation="MVISVFDLFKVGIGPSSSHTVGPMWAANRFLLALDARRLIERVE
                     RVEVQLYGSLALTGKGHATDRAILLGLAGEVPDEVDPDAAGELVRAIRETGALRLMGR
                     REIAFSETLDLQFRQLETMTKHPNGMRFLAHDGDGAVLLEEVYFSVGGGFVLSDAETE
                     RPRGEGRNATPPYPFATAEELLALGERHKMTIAEIVLANERAWREPAEIRAGLDALWQ
                     AMEASIERGCRQGGELPGGLRVRRRAARLFQELCERPEAGLRDQLTVLDWVNLYALAV
                     NEENAAGGRVVTAPTNGAAGVIPAVLAYYTRFVPGSGAEGVRTFLLTAAAIGQLYKEN
                     ASISAAEVGCQGEVGVACSMAAGALCAVMGGSNQQVENAAEIGMEHNLGLTCDPIGGL
                     VQIPCIERNTMGAVKAINAARLALRGDGEHHVSLDKVIDTMRETGRDMSAKYKETAQG
                     GLAVNLVEC"
     gene            3876..>4642
                     /locus_tag="P5558_21930"
     CDS             3876..>4642
                     /locus_tag="P5558_21930"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRY27042.1"
                     /translation="MAQAKPVQAVSGEARGRGFFTRSLAEADPGIARAVDQELDRQQQ
                     QIELIASENIVSRAVLEAQGSVMTNKYAEGYPTKRYYGGCEYVDVAESLAIERAKQLF
                     GSSYANVQPHSGAQANGAVMLALLEPGDTILGMSLDAGGHLTHGARPALSGKWFKAMH
                     YGVRPEDGLIDYDAVERLTLEHRPKMLIAGASAYSRVLDFERFRAIADKVGAWLMVDM
                     AHIAGLVATGHHPSPLRHAHVVTTTTHKTLRGPRGGLV"
BASE COUNT          687 a         1645 c         1625 g          685 t
ORIGIN      
        1 cgttgcgcgt cttgagctcc ttgacggtgt cgacaccggc agcctcgagc agctcggcga
       61 actgcttgcc gatgcccttg atccgcatca gatcggccat gttggcccag agcaggatgc
      121 gggaagcgtc gatgccggtc ttctgcgcca ggagctggcg ccccctggga tcggccgccg
      181 ccttgagcag ggccgaggtc gaggcgatgc cggcgtcgcg cagcttggcc ccataggcgc
      241 cgccgatgcc ctcgatgtgc tcgatgctgt agcccatgtc gttgtccccc ccttcggcag
      301 acccacagcc gatgtgcggg ctcggcagcg cctaggcaag catttcctca cccgacgatg
      361 tcggccggcc gcggccatcc cccgttcggc tgttgatcga agtggccaag cgccctactc
      421 ccgcaccaca caacaaggag cgctgagatg cgggtgctga tgaccggagc ggccgggggc
      481 gtggcgacga tgttgcggcc acttcttgtc gggctctatg acgaggtggt gctgagcgac
      541 cgccgggcgc ccgaggggct ggcggcgcac gagcggttcg tggccgccga cctcggcgat
      601 ccggattctc tcgccgccgc ctgtgacggg gtcgacggga tcatccacct cggcggtcag
      661 gcgtacgagg ccgaatggga ggtgatccac agcgccaata tcaccggttg ctggaacctg
      721 ttcgaggcgg cccgccaggc tggggtcgag cgcgtcgtct tcgcctcctc caaccacgcc
      781 atcggcttct atcccaggcg cgatcggatc ggtgtcgacg agccggtcag gcccgacagc
      841 cgctatggca tctccaaggc gttcggcgag gcggtgggct cgttctatgc cgacaagttc
      901 gggctcaggg tgctgtcgat ccgcatcggc aatgtcgggc ccgtgccgct cgacaagcgc
      961 cgcctctcca tctggatcca ccaggaggat ctgctcagcc tgatcaggat cggcctcgag
     1021 caccccgagc tgcacaacgc gatcgtctat ggcgtgagcc agaacgagcg atcctggtgg
     1081 gacaaccgga ccgcctaccg gctcggctat gcgccgaggc accgggccga ggatcatgtc
     1141 gacgcggccc tggccgccca ggccaagctg ccgcccgacc cgatcggcga tctcttccag
     1201 ggcggcacgt tctgtgctgc cgagttcgcg ggcgacctga agcgaacctc gtcgtagcgg
     1261 cccggatcaa ccccgcggtg ctcgggtatt aaccgaagga tcgccgcacg ttagcttcat
     1321 cgtattcgag tatcgatcga ggcagttgcc gctcggcggg ccgcccggcc ccgttgcatc
     1381 ggccatcggg ctttggcgtc atcgcaccgg ttgaccagcc gggatcccgg gagcctgtcc
     1441 gaaagcatga ccgcgaacga tgccgtgccg cccatccggc tcgatcgcta tctggcgctg
     1501 ctggcgctgc tgctcgcgat cgtgctcgcc tttgtcgtgc tcggcgagcg gcagaaggcc
     1561 gcgatccgcc cgacgccggt caccggcgcc ctgccgccac cgcccgaccc gcgcctcgcc
     1621 gccctggccg gggagcgcga ccggctcctc acggagcagg ccaggctcgc cgacgggctc
     1681 gccaccgccg agcacgccct ggcggcggcc gaggccgaca tcgagcgcct gaaagcgcag
     1741 gtcgacaagc tcgagcgcga cctcagcgcc gaggtcgagg cccgcgcggt gctcgagcga
     1801 cgcctggccg aggtggccgc cgagcgcgac cggctggcga cggaagtcca ggctctcacc
     1861 cggatccgcg agagtctcga ggcggagctg gcagcgctcg cggccgccac gccaatccct
     1921 ccggcgccgc cttccgccgc acggcaagag ccgccggccg cacccgcgcc tgagccgaca
     1981 ccggcaccgg caccggcagt ggcggacctg ctcccggggc cggacatcgc acccgaagca
     2041 cccggccctg cggtgcatgg cccgattccc ccggcgggcg aatcggccac cggcgaccgc
     2101 cccggtcggc tggtgatcct cgatggcagg cgcaccggcg tcgcggatgg gatcaaggcc
     2161 taccaggccg gcgagtacga cgcggccgca cgcatctggg gcaaggcggc ggccgaaggc
     2221 gaggcgcgcg cccagttcca cctggggtcg ctgctgttcg aggggcggac cggcccgccg
     2281 gacctcgtca tggcctatgt ctggctgagc cgggcggtga cgggtgggca tctgccggcc
     2341 atcgaggtgc gccgccgggt gcgcgccgcc atgaccgagg cgcagtacga ggccgccctc
     2401 gcgatcctcg aggccggctg acctgcatcg cccccgggca tggcggcaac gccgtcccgc
     2461 tgctacgatg cccggtcagc tcgggggtgt ctcatggtca tcagcgtctt cgacctcttc
     2521 aaggtcggca tcggcccgtc cagctcgcac accgtgggcc cgatgtgggc cgccaaccgc
     2581 ttcctgctgg cgctcgacgc gcggcggctg atcgagcggg tcgagcgggt ggaggtccag
     2641 ctctacggct cgctggcgct caccggcaag ggccacgcga cggaccgcgc gatccttctg
     2701 gggctcgccg gcgaggtgcc ggacgaggtc gaccccgacg cggcgggaga gctggtgcgg
     2761 gccatccgcg agaccggtgc gctgcgcttg atgggccggc gcgagatcgc cttcagcgag
     2821 acgctcgacc tgcagttccg ccagctcgag accatgacca agcatccgaa cggcatgcgc
     2881 ttcctcgccc atgacggcga cggtgcggtg ctgctcgagg aggtctattt ctcggtcggc
     2941 ggcggcttcg tcctgagcga cgccgagacc gagcgaccac ggggcgaggg ccgcaacgcg
     3001 accccgccct atcccttcgc caccgcggaa gagctgctgg ccctgggcga gcggcacaag
     3061 atgaccatcg ccgagatcgt cctcgccaac gaacgggcct ggcgcgagcc ggcggagatc
     3121 cgcgccggcc tggatgcgct ctggcaggcc atggaggcct cgatcgagcg cggctgccga
     3181 cagggtggcg agctgcccgg cgggctgcgc gtccgccgcc gggcggcacg gctcttccag
     3241 gagctctgcg agcgaccgga agccggcctc agggaccagc tcaccgtgct cgactgggtc
     3301 aatctctacg cgcttgctgt caacgaggag aatgccgcgg gcgggcgggt cgtcacggcg
     3361 ccgaccaacg gtgcggccgg cgtcatccca gcggtgctcg cctactatac ccgcttcgtg
     3421 cccgggtccg gtgcggaggg ggtccggacc ttcctgctca cggccgcggc gatcggccag
     3481 ctctacaagg agaacgcctc gatctcggcg gccgaggtcg gctgtcaggg cgaggtgggc
     3541 gtggcctgct cgatggccgc cggtgcgctc tgcgcggtca tgggcggcag caaccagcag
     3601 gtcgagaatg ccgccgagat cggcatggag cacaatctcg gcctcacctg cgacccgatc
     3661 ggcgggctcg tgcagattcc ctgcatcgag cgcaacacca tgggggcggt gaaggccatc
     3721 aacgccgccc ggctggcgct gcgcggcgac ggggagcacc atgtctctct cgacaaggtc
     3781 atcgacacca tgcgagagac cgggcgcgat atgagcgcca agtacaagga gaccgcccag
     3841 ggcggtctcg ccgtcaatct ggtggagtgt tgagcatggc ccaggcaaag ccggtccaag
     3901 cggtatccgg ggaggcgcgc ggccgcggct tcttcacccg cagcctagcc gaggccgatc
     3961 ccgggatcgc gcgcgccgtc gaccaggagc tcgaccgcca gcagcagcag atcgagctga
     4021 tcgcgtccga gaacatcgtc agccgcgcgg tgctcgaggc gcagggctcg gtgatgacca
     4081 acaagtacgc cgaaggctac ccgaccaagc gctactatgg cggctgcgaa tatgtcgacg
     4141 tcgccgaatc gctggccatc gagcgtgcga agcagctctt cggttctagc tatgctaatg
     4201 tgcagccgca ttcgggtgcg caggccaacg gggccgtgat gctggcgctg ctggagcccg
     4261 gcgacaccat cctcggcatg tcgctcgacg ccggcggcca tctgacgcac ggcgcccggc
     4321 ctgccctctc gggcaagtgg ttcaaggcga tgcattacgg tgtgcggccg gaggacgggc
     4381 tgatcgacta cgacgccgtc gagcggctga ccctggagca tcggccgaag atgctgatcg
     4441 ccggcgcctc cgcctattcg cgggtcctcg acttcgagcg gttccgcgcc atcgccgaca
     4501 aggtcggcgc gtggctgatg gtcgacatgg cgcatatcgc cgggctcgtc gccaccggcc
     4561 atcacccctc cccgctgcgc cacgcccatg tggtgaccac caccacccac aagacgctgc
     4621 ggggcccgcg cggcggcctc gt
//
LOCUS       DAOTFI010000314         4611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_22208_length_4611_cov_6.461589, whole genome shotgun sequence.
ACCESSION   DAOTFI010000314 DAOTFI010000000
VERSION     DAOTFI010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22208_length_4611_cov_6.461589"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..102)
                     /locus_tag="P5558_21935"
                     /pseudo
     CDS             complement(1..102)
                     /locus_tag="P5558_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009808286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(570..833)
                     /locus_tag="P5558_21940"
     CDS             complement(570..833)
                     /locus_tag="P5558_21940"
                     /inference="COORDINATES: protein motif:HMM:NF025304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY27043.1"
                     /translation="MSEGRYRHLTLEEREQIAVWRPEGISQAEMARRLGRSSSTGNRE
                     LARNRLPSGGYQPSFAEGSYLARRERAACWSGTSGSSGSWSIV"
     gene            complement(1151..1540)
                     /locus_tag="P5558_21945"
     CDS             complement(1151..1540)
                     /locus_tag="P5558_21945"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY27044.1"
                     /translation="MDANIFMYAAGAPHPHKAPSVAWLRRVAAGELAAATDAEVLQEV
                     LHRYRSIDRWDDGCRVYDLARRVVPMVLPVTVDTLDGARELMDSDSRLSARDAIHAAA
                     AIANGCHAICTYDRAFDRITGLTRIEP"
     gene            complement(1546..1812)
                     /locus_tag="P5558_21950"
     CDS             complement(1546..1812)
                     /locus_tag="P5558_21950"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRY27045.1"
                     /translation="MLEVAMELSHKTTILLSADLHRRLTALARERRQSLGELVREACR
                     RQYGLADRADRLAAVDALAGLQLPVGPVDAMIRESVAPPRDLPS"
     gene            complement(1854..2291)
                     /locus_tag="P5558_21955"
     CDS             complement(1854..2291)
                     /locus_tag="P5558_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRY27046.1"
                     /translation="MQTLLASWQFWAIGAAFFAALTAIFAKLGVAGVPPDFATFVRTT
                     VILAVSAAIVTATRQWQPPATVALRTWLVLALSGLATGASWLCYFRALDLGDAARVAP
                     LDKLSVVLVAILATLLLGEKLSPANWLGVTLIAAGAMLVAWRG"
     gene            complement(2353..2925)
                     /locus_tag="P5558_21960"
     CDS             complement(2353..2925)
                     /locus_tag="P5558_21960"
                     /inference="COORDINATES: protein motif:HMM:NF002099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HRY27047.1"
                     /translation="MDLGRISNTTWLAIAAAIVVIQALALHFMGQPWICECGTIRLWE
                     GDVSGPENSQHLTDWYTFSHIIHGQAFYLLLWLATPKLPLATRFAIAIGLEAAWEIAE
                     NSTMVIERYREQALAQGYFGDSIVNSISDTLAAAAGFALARLLPVKASIALAITLELF
                     AGYMIRDNLTLNIIQLIHPVEAISRWQTGM"
     gene            complement(2987..4189)
                     /locus_tag="P5558_21965"
     CDS             complement(2987..4189)
                     /locus_tag="P5558_21965"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HRY27048.1"
                     /translation="MDSIENPPRTWLMLGGKAGDNTQITALAEGLGWPFETRRMAYRR
                     TEILTNLLAGPTVAGLVPERSDPLQPPWPELVISAGRRNEPLIRWIQLQAARDPALPR
                     VRLVHVGRPWALHECFDLIVTTPQYRLPTKPNILHNELPLQRVNAGRLQREAAAWQGR
                     LAHLPAPHIALLVGGHAGPYNFDRENGALLGCHASRMAAERGGSLLVTTSARTPEAAI
                     DAFASQLRVPHSLHRFVRFQPPEENPFFAFLGMASEIIVTSDSMSMLAEAVATGKPTH
                     IFDLARGPRSNRPRRPADGSIAPRTLEERLADFRTQAIMYKIGMHAVPRRLTRDVRII
                     HDRQVAAGRAVWLGHPWPDDRPNPPPLTDLPRAVEAVRRLFGPYPPPPPALPDSRWPE
                     LIQRVFQG"
     gene            complement(4207..>4611)
                     /locus_tag="P5558_21970"
     CDS             complement(4207..>4611)
                     /locus_tag="P5558_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HRY27049.1"
                     /translation="PRTRRLVIEEGGFLYDADSYADDLPYWVARPAGGHHLIVPYTLD
                     ANDMRFATTQGFNSGDQFFAYLKDSFDVLWNEGRTSPKMLSVGLHCRLVGRPGRLAAL
                     QRFLDHIDRHEHVWVCRRLDIARHWHAHHAPA"
BASE COUNT          710 a         1347 c         1854 g          700 t
ORIGIN      
        1 gccgctccag ccgcgatcgt cgcgagccac gacccaaaga tagccggtct tggtccggcc
       61 gcgtcccgga tcgagcaccg gcacgggcgt ctcgtccatg aatggccaga aggccattca
      121 tccgatcaat gaaaagggcg cggccagcag gccgcgaagg gagcctggcc gccagggtca
      181 gccgcgaggt gcgctcgtgc agcaccggaa ccggctgcgt gcgcctgcag aacatgagat
      241 cggcctccca atcgccgagc ttctcgcggt tctggacagc ttcgggccga tcgtggatcg
      301 agcgccgatc agtgatcgtg ctcttcgtct cgcggcgctt gcgccgaccg cgccggagct
      361 tgccgcgccg cagcagttgc cagagcttct cgactttctg ccgggcccga tagatgaagc
      421 gatagatggt ctccgcgccc agcccacgca gccctggctc gttgccggcc ttgagccatc
      481 gatcgcagga ggcgatcgat ccatccctgc aagggccatg gtcaggagac catggcggcc
      541 cgcgatctgc tcgggcgtcc agccctcggt cagacgatcg accacgaacc gctcgagccg
      601 ctcgtcccgc tccagcaggc cgcccgttcg cgccgggcca gataagaccc ttccgcgaag
      661 ctcggctggt agccgcccga gggcagccgg ttgcgcgcca gctcgcggtt gccggtcgac
      721 gacgaccgcc ccagccgtcg cgccatctcg gcctggctga tcccctccgg ccgccagacc
      781 gcgatctgct cgcgctcctc caacgtcaga tgacggtatc gaccctcgct catcctccca
      841 ccctccaagc gttgccagca ccctaccacc tatgtgcttg aagcgggaat ccacccctgc
      901 cgcatctggt agccgagttg agagtgagtt cggagtcgac gccgacagac aactcgcagg
      961 ctagcgccga gagtcaggtt ctcgccaagc caatagaatg atggggcgtg gggaagatca
     1021 tcttcaccac atgttctttg cgttttcgat ccatcggggg ggcagcgagc tttttggaac
     1081 tggccgttgc tcggtgccga ccgctcaccg ccgccacgcg acgagcacgc ggtcaggtcg
     1141 gttggcgggg tcagggctcg atccgggtga ggccggtgat gcggtcgaag gcgcgatcgt
     1201 aggtgcagat ggcgtggcaa ccgtttgcga tggcggcggc ggcgtggatg gcgtcgcggg
     1261 cggacaggcg cgaatcgctg tccatcagct cgcgggcgcc gtcgagggtg tcgacggtga
     1321 cgggcaagac catcggcacg acgcggcggg cgaggtcgta gaccctgcag ccgtcgtccc
     1381 agcggtcgat cgaccggtag cgatggagga cttcctgcag cacctcggca tcggttgcag
     1441 ccgccagctc gcccgcagcg acccgtcgga gccaggcgac gctcggcgcc ttgtgcggat
     1501 ggggcgcacc ggcggcgtac atgaagatgt tggcgtcgat gaggatcagg acgggaggtc
     1561 ccggggcggg gcgacgcttt cacggatcat ggcgtcgacc gggccaacgg gaagctgcag
     1621 cccggcgagt gcgtcgacgg cggcgaggcg gtctgcccgg tcggccaggc cgtattgcct
     1681 gcggcaggcc tcgcggacga gttcgcccag gctctgtcgc cgctcgcggg caagggcggt
     1741 gagccgccgg tgcagatcgg ccgagagcag gatcgtcgtc ttgtgcgaaa gctccatagc
     1801 gacctccagc atgatagatg gctcccgggc ccggcggcgt caacggtcgt tcactaaccc
     1861 cgccatgcca ccagcatggc gcctgcggcg atgagggtga cgccgagcca gttggcgggg
     1921 ctgagctttt cgccgaggag gagggtggcg aggatggcga cgaggacgac gctgagcttg
     1981 tcgagggggg cgacgcgggc ggcgtcgccg aggtcgaggg cgcggaagta gcagagccag
     2041 gaggcgccgg tggcgaggcc ggagagggcg aggacgagcc aggtgcgcag cgccacggtg
     2101 gcgggtggct gccattggcg ggtggcggtg acgatggcgg cgctgacggc gaggatgacg
     2161 gtggtgcgca cgaaggtggc gaagtcgggt gggacgccgg cgacgccgag cttggcgaag
     2221 atggcggtca gcgcagcgaa gaaggcggcg ccgatggccc agaactgcca ggaggcgagg
     2281 agggtctgca tgggtcggtg atccgtccgc agaaagccgt tggggctcgg ggaagatcat
     2341 cttccccgat tttcacatcc ccgtctgcca gcgggagatg gcttcgacgg ggtggatgag
     2401 ctggatgatg ttgagggtga ggttgtccct gatcatgtag ccggcgaaga gctcgagggt
     2461 gatggcgagg gcgatggagg ctttcaccgg gaggaggcgg gcgagggcga agccggcggc
     2521 ggcagccagg gtgtctgata tggagttgac gatgctgtcg ccgaaatagc cctgggccag
     2581 ggcctgctcg cggtagcgtt cgatgaccat ggtggagttc tcggcgatct cccaggcggc
     2641 ttcgaggccg atggcgatgg cgaagcgggt ggcgagaggg agcttcgggg tggcgagcca
     2701 gaggaggagg tagaaggcct gaccgtggat gatgtggctg aaggtgtacc agtcggtcag
     2761 gtgctgggag ttctccggcc cggagacgtc accttcccag aggcggatgg tgccgcattc
     2821 gcagatccag ggctggccca tgaaatggag ggcgagcgcc tggatcacga cgatggcggc
     2881 ggcgatggcg agccaggtgg tattcgagat gcgacccagg tccatgcagg tctccgccgc
     2941 caaattccgg tagggccagg ggaggccgtg cctcccctga acttctctac ccctgaaaca
     3001 cccgctggat aagctcaggc cagcggctgt ccggcagggc tgggggcggc ggcgggtacg
     3061 ggccgaagag gcggcggacg gcttcgacgg cgcggggcag gtcggtgagc ggcggcgggt
     3121 tcgggcggtc atccggccag gggtggccga gccagacggc gcggccggcc gcgacctggc
     3181 ggtcgtggat gatgcggacg tcgcgggtca ggcgcctcgg cacggcgtgc atgccgatct
     3241 tgtacatgat ggcctgggtg cggaagtcgg cgaggcgttc ctcgagggtg cgcggggcga
     3301 tgctgccgtc ggcgggccgg cgcgggcggt tcgagcgggg gccgcgggcg aggtcgaaga
     3361 tgtgggtggg tttgccggtg gccacggctt cggcgagcat cgacatgctg tcggaggtga
     3421 cgatgatctc gctcgccatg ccgaggaagg cgaagaaggg attttcttcc gggggctgga
     3481 agcggacgaa gcggtgcagg ctgtggggga cccggagctg gctcgcgaag gcgtcgatgg
     3541 cggcttcggg cgtgcgcgcg ctggtggtga cgaggagcga gccgccgcgc tcggcggcca
     3601 tgcggctggc gtggcagccg agaagcgcgc cgttctcgcg gtcgaaattg taggggccgg
     3661 catggccgcc cacgaggagg gcgatgtggg gcgcagggag gtgggcgagg cggccctgcc
     3721 aggcggcggc ctcgcgctgg agccggccgg cgttgacgcg ctgcaaaggc agctcgttgt
     3781 ggaggatgtt gggcttggtc ggcaggcggt attgcggcgt ggtgacgatg aggtcgaagc
     3841 attcgtgcag cgcccagggg cggccgacat ggacgaggcg gacgcgcggg agggcggggt
     3901 cgcgcgcggc ctgaagctgg atccagcgga tgagcggctc gttgcggcgg ccggcggaga
     3961 tgacgagctc gggccagggg ggctggagcg ggtcggagcg ctcgggcacg aggccggcga
     4021 cggtcgggcc ggcgagcagg ttggtgagga tctcggtccg gcggtaggcc atgcgccggg
     4081 tctcgaaggg ccagccgagg ccttcggcga gcgcggtgat ctgggtgttg tcgccggcct
     4141 tgccgcccag catgagccag gtgcgcggcg ggttctcgat gctgtccaag gtccggcggg
     4201 ccagggtcag gcgggggcgt ggtgggcgtg ccagtggcgg gcgatgtcga ggcggcggca
     4261 gacccagaca tgctcgtgcc ggtcgatatg atcgaggaag cgctgcaggg cggcaaggcg
     4321 cccggggcgg ccgacgaggc ggcagtgcag gccgacggag agcatcttcg ggctcgtccg
     4381 cccctcgttc cagagcacgt cgaagctgtc cttgaggtag gcgaagaact ggtcgccgga
     4441 gttgaagccc tgggtggtgg cgaagcgcat gtcgttggcg tcgagggtgt aggggacgat
     4501 caggtgatgg ccgccggcag gcctcgcgac ccagtagggg aggtcgtcgg cgtaggagtc
     4561 ggcgtcgtag aggaagccgc cctcctcgat gacgaggcgg cgggtccggg g
//
LOCUS       DAOTFI010000315         4607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_22236_length_4607_cov_6.668937, whole genome shotgun sequence.
ACCESSION   DAOTFI010000315 DAOTFI010000000
VERSION     DAOTFI010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22236_length_4607_cov_6.668937"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="P5558_21975"
     CDS             complement(<1..776)
                     /locus_tag="P5558_21975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27050.1"
                     /translation="MPPAGTPATTEATIEAGRIRRNWLLAAPALALLLIGASGPLLIV
                     LVYSFLEPGDYSGVIWKFTPRAWFEIFLKRDIFDGTLGVADAHLTIFWRSVRLSLLTT
                     LITILLGFPTAWFIATRPRSQRALWLFLITIPFWTNLLIRTFAIMEVIRNQGVMNTVL
                     LQAGLIAEPIQILYTDTAVLIGMTYVYLPLMVLPLYAAIDRFDFRLAEAGYDLYASRL
                     QVLRRVILPLVRPGLIAGSILVFVPSLGAYVTPRVLGGGK"
     gene            complement(782..1897)
                     /locus_tag="P5558_21980"
     CDS             complement(782..1897)
                     /locus_tag="P5558_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY27051.1"
                     /translation="MVPPSLQPAAGPSETPRIAVEARNASKAFGQGDALTRALDDVSV
                     AIRKGEFFTLLGPSGCGKTTLLRLIAGFDTPTGGSILLNGRDITADPPNRRPVNTVFQ
                     SYALFPHLTVLQNIRFGLDMLGRKRAEADARAAEMLALVRLEGFASRRTAQLSGGQQQ
                     RVALARALAPEPEVLLLDEPLSALDLKLRKEMQRELKRLQRETGITFVFVTHDQEEAL
                     TMSDRIGVMQGGRILQIGPPREIYNQPANRFVADFIGETNFLDGEVGAIAGDTATIEL
                     AAGSVTVRSAGLDRGQKVTLAIRPEQVRIGEAEGLPAVVTGNVYFGTDTHCHLELSGG
                     AGVVARLQSAADDATPLGEGTAVRVSFTPGSVQVLAD"
     gene            complement(1961..2287)
                     /locus_tag="P5558_21985"
     CDS             complement(1961..2287)
                     /locus_tag="P5558_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27052.1"
                     /translation="MAGIVVQLARARHREARMVPCVLPHRPAAMAVAGDHVRLEAQLL
                     ARRHQLLGLLPGAERTARNEIWHGHKVGTERNTDRRFSMMIRSTIATERHGRPAFRME
                     PAAFFA"
     gene            complement(2465..3538)
                     /locus_tag="P5558_21990"
     CDS             complement(2465..3538)
                     /locus_tag="P5558_21990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY27053.1"
                     /translation="MKSPGITAIIVNHNSETEIVKAIRKLEAQDGLVDAILVIDNAST
                     DNSVPAAKAASPRVEVLELGENLGPAISRNRGMQAAKTPLVLLVDDDVYVAPGCIARL
                     LAHLDDPRVAAVVPRLILYPDNELIQLDGAETHLVGSMVLNNARMEAATTPAEVSHIG
                     AFSTSCVLARRDVMLAAGGMDENYFIYLEDQEFGLRLRGLGHKLVCDARALAYHDRGA
                     GTPNLSFRDAGEYPKRRAYLTMRNRWQTILIHYQPRTLVLLAPVLLLYEIASLAMAVR
                     KGWLSQWWQAWVWQWQNRQSLCERRRFVARNRRLSEASSLVGGPLPLAPGLIGKGFEA
                     RAVAALTAIISGYGRLVRPLLPR"
     gene            complement(3535..4137)
                     /locus_tag="P5558_21995"
     CDS             complement(3535..4137)
                     /locus_tag="P5558_21995"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRY27054.1"
                     /translation="MCDAWRFRSMNDVAVEHDLVRMLGGAPTVLRTCAKRAVEIIRPR
                     RVMALRMVVETAKQRDSRVRLDHATDRLDQPRALVDWRVSDSDRPGLDRLRRTLARLL
                     RQSGHGEMIDDWNSHENGWPPSMDGGRHDMGNNRMHDDPTRGVVDADARVDGLENLHV
                     AGSSVFPTGSNVDPKPTIVALTHRLAEHPRALTSHAADGP"
     gene            complement(4107..4469)
                     /locus_tag="P5558_22000"
     CDS             complement(4107..4469)
                     /locus_tag="P5558_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27055.1"
                     /translation="MASLAEGIGIGLLIPFLAVLMGAATPDGGFVEIADRYAALFDED
                     TRLVMVSITIVTPIVGKCLLDFLYVRLPAILPSAAREPAGPSPTSFAGACPKQRRTTT
                     SAAVAPATGCAMPGGSAA"
BASE COUNT          740 a         1467 c         1683 g          717 t
ORIGIN      
        1 ttcttgccgc cgccgagcac gcggggcgtc acgtaggcgc cgagcgacgg cacgaagacg
       61 aggatcgagc cggcgatcag cccggggcgg accagcggca ggatcacccg gcgcagcacc
      121 tggaggcgcg aggcgtagag gtcgtagccg gcctcggcga ggcggaagtc gaagcggtcg
      181 atggcggcgt agagcggcag caccatgagc ggcaggtaga cataggtcat gccgatcagc
      241 acggcggtgt cggtgtagag gatctggatc ggctcggcga tcaggcccgc ctgcagcagg
      301 accgtgttca tgacaccctg gttcctgatc acctccatga tggcgaaggt gcggatcaag
      361 aggttggtcc agaacgggat ggtgatgagg aagagccaga gtgcccgctg gctccgcggg
      421 cgggtggcga tgaaccaggc ggtcgggaag cccaggagga tggtgatgag cgtggtcagc
      481 agcgacaggc gcaccgagcg ccagaagatc gtcaggtgcg cgtcggcgac gccgagggtg
      541 ccgtcgaaga tgtcgcgctt gaggaagatc tcgaaccagg cccggggcgt gaacttccag
      601 atgacgcctg aataatcgcc gggctcgagg aacgagtaga cgaggacgat caggagcggg
      661 ccggaggcgc cgatcagcag gagggcgagt gccggggcgg cgagcagcca gttgcggcgg
      721 atgcgcccgg cctcgatcgt ggcctccgtg gtggctgggg tgccggctgg gggcatggag
      781 gtcagtccgc caggacctgg accgagccag gggtgaacga gacgcgcacg gccgtgccct
      841 cgccgagcgg cgtcgcgtcg tcggccgcgc tctgcaggcg ggcgacgacc ccggcaccgc
      901 ccgaaagctc gaggtggcaa tgcgtgtcgg tgccgaaata gacattgccg gtgaccacgg
      961 cgggcagccc ttccgcctcg ccgatgcgga cctgctcggg gcggatcgcc agggtgacct
     1021 tctggccccg atcgaggccc gccgagcgaa cggtcacgga gccggcggcg agctcgatcg
     1081 tcgcggtgtc gccggcgatc gccccgacct cgccatcgag gaaattggtc tcgccgatga
     1141 agtccgcgac gaagcggttg gccggctggt tgtagatctc gcgcgggggc ccgatctgca
     1201 ggatcctgcc gccctgcatc acgccgatcc ggtcggacat ggtgagcgcc tcctcctggt
     1261 cgtgggtgac gaagacgaag gtgatgccgg tttccctttg cagccgcttg agctcgcgct
     1321 gcatctcctt gcgcagcttg agatcgaggg cggagagcgg ctcgtcgagc agcaggacct
     1381 cgggctcggg ggccagcgct cgcgccagcg ccacgcgctg ctgctggccg ccggaaagct
     1441 gggccgtccg gcggctggcg aacccctcga gccgcaccag cgccagcatc tcggcagccc
     1501 gggcgtccgc ctcggcccgc ttgcggccga gcatgtcgag gccgaagcgg atgttctgga
     1561 gcacggtcag atgcgggaag agcgcgtagc tctggaacac ggtgttgacc gggcgccggt
     1621 tcggcgggtc ggcggtgatg tcccggccat tcaggaggat ggacccgccg gtcggcgtgt
     1681 cgaagcccgc gatcaggcgc agcagcgtgg tcttgccgca accggagggg ccgagcaggg
     1741 tgaagaactc gcccttgcgg atcgcgaccg agacgtcgtc gagggctcgc gtcagggcgt
     1801 cgccctggcc gaacgccttg ctggcattcc tggcctcgac ggcgatgcgc ggggtctcgc
     1861 tcgggccggc ggcgggctga agcgacggtg gaaccatggc caggccttcc cttttccgtg
     1921 accggctggc cagcggcccc tgcgccacga tgcggaaaga ctaggcgaag aatgccgctg
     1981 gctccatccg gaacgcgggg cggccatggc gttcggtggc aatggtgctg cggatcatca
     2041 tcgagaagcg cctgtcggtg tttcgttcgg tgcccacctt gtgcccatgc catatctcat
     2101 tgcgggcggt ccgttcggct ccaggcagca gccctaggag ctggtggcgg cgggccagga
     2161 gctgcgcttc gagccggaca tgatcgcctg ccacggccat ggcggctggc cgatgcggca
     2221 gcacgcacgg caccatacgg gcttctcgat gccgcgctct tgccagttgc acgacgatgc
     2281 ccgccatgcc cggaggttcg agctcgggca ctcggcctgc ccttcttcgc tggaccgggg
     2341 ccttgtcacg gtgaccgcgt gaggcatcgg gccgcggacg accggtgctc acatcggcaa
     2401 accgagctgc acaccgatcg ccttgccttt ctcacctgcg gtcgccgtgc agatgcgttg
     2461 gtcgttaccg cggcaggagg gggcggacga ggcggccgta gccgctgatg atcgcggtca
     2521 gggccgcgac cgctctggcc tcgaagccct tgccgatcag ccccggcgcc agaggcaggg
     2581 gaccaccgac cagcgagctg gcttcgctga gccggcgatt gcgggcgacg aaccggcgtc
     2641 gctcgcacag gctttgccgg ttctgccact gccagaccca ggcctgccac cactggctga
     2701 gccagccttt ccgcaccgcc atcgccagcg aggcgatctc gtagagcagc aggacggggg
     2761 cgaggaggac gagggtgcgc ggctggtagt ggatgaggat cgtctgccag cgattgcgca
     2821 tggtgagata ggcccggcgc ttggggtact cgccggcatc gcggaacgac aaattcggcg
     2881 tgccggcgcc acgatcgtga taggcgagcg cgcgcgcgtc gcagaccagc ttgtgcccga
     2941 gcccgcgcag gcgcaggccg aattcctgat cctcgagata gatgaagtag ttctcgtcca
     3001 tgccgccggc cgccagcatg acgtcgcgcc tggccagcac gcaggaggtc gagaaggcgc
     3061 cgatgtggct gacctcggcc ggcgtggtgg ccgcctccat acgggcattg ttcaggacca
     3121 tggagccgac cagatgggtt tcggcgccgt cgagctggat cagctcgttg tcgggataga
     3181 ggatcagccg cggcacgacc gcggccaccc gggggtcgtc gaggtgggcg agcagccggg
     3241 cgatgcagcc gggtgccacg tagacatcgt cgtcgaccag gaggaccagc ggtgtcttcg
     3301 ctgcctgcat gccacgattg cgcgagatcg ccgggccgag attttcgccg agctcgagga
     3361 cctcgacgcg cgggctcgcc gccttcgccg ccggcacgct attgtcggtc gaggcattgt
     3421 cgatcaccag gatggcgtcg acgaggccgt cctgggcctc gagcttgcgg atcgccttga
     3481 cgatctcggt ctcgctgttg tggttgacga tgatcgcggt gatgccgggg ctcttcatgg
     3541 cccgtctgcc gcatggctgg tcagggcgcg cggatgctcg gcgaggcgat gcgtcagcgc
     3601 cacgatcgtc ggtttcgggt cgacgttgga gccggtcgga aagaccgagc tgccggcgac
     3661 atgcagattt tcgaggccat ccacgcgcgc gtcggcatcg accacacccc gggtcggatc
     3721 gtcgtgcatg cggttattgc ccatgtcatg ccgtccgccg tccatggatg gtggccagcc
     3781 gttctcgtgc gaattccagt cgtcgatcat ctccccatgg ccgctctgac gcaagagccg
     3841 cgccagggtc cggcgcaaac gatcgagccc ggggcggtca ctgtcgctga cccgccagtc
     3901 gaccagggcc cgcggctggt cgagccgatc cgtagcatga tcgagccgga cccggctgtc
     3961 ccgctgcttc gccgtctcta ccaccattcg caacgccatg acccgcctcg gtctaatgat
     4021 ctcgacggca cgcttggcac aggtgcgcag cacggttggc gctccaccga gcatcctgac
     4081 gagatcgtgc tcgacagcga cgtcattcat gctgcggaac cgccaggcat cgcacaaccg
     4141 gttgcgggcg cgactgccgc tgaagtggta gtccggcgtt gtttcggaca ggcgccggcg
     4201 aatgaagtag gcgacggtcc cgctggttcc ctcgccgcgc tgggcaggat ggccggcagc
     4261 cgcacataga ggaagtcgag caggcacttg ccgacgatcg gcgtgacgat ggtgatgctg
     4321 accatgacca gccgcgtgtc ctcgtcgaac aacgcggcgt agcggtcggc gatctcgacg
     4381 aagccgccat ccggcgtggc ggcccccatc agcactgcca ggaacgggat caggaggccg
     4441 atgccgatgc cctcggccag cgatgccatc acgccgaggg ctgcgacgtc cggcagggat
     4501 ccagggacaa gggcagagga agccgacaat caggcgcagc gcgcgaatgg gcaaacgggt
     4561 ctcgcttcga gggccaaggg acgaggattt tgcgattggc accctaa
//
LOCUS       DAOTFI010000316         4577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_22413_length_4577_cov_6.942724, whole genome shotgun sequence.
ACCESSION   DAOTFI010000316 DAOTFI010000000
VERSION     DAOTFI010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22413_length_4577_cov_6.942724"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="P5558_22005"
     CDS             <1..243
                     /locus_tag="P5558_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY27056.1"
                     /translation="VALMFRYVFAFRLFAEVWLLTGGGPARQTEVLATYLYRHAFRYQ
                     EFGVASAIGWLMVVGTLLISAVYLREMYKRMLSADA"
     gene            236..1066
                     /locus_tag="P5558_22010"
     CDS             236..1066
                     /locus_tag="P5558_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY27057.1"
                     /translation="MREGPTRRAGKLAALALIAIWSGLPILIVVLSSFKPPREILAFP
                     PKLLFSPTFENYRALIVRWPEFFEALQSSLIVTTGATILTVVVTTLAGYVYARVRSRK
                     LAASAFMMIVIRMVPPIVVTLPLFPVVNWLRLNDTHTVLILLYATFFVSLNTWIMKAF
                     IDQIPVELEEAAIIDGAGVWQILRHVIVPLAAQGMIAASTFVLIFSWNEFLFAFIFTT
                     TEAKTAPLILSEMVGAVDGVEWGVLFAAATLQLLPILIFVIAVQKYVIAGMTAGSLKG
                     "
     gene            1094..1942
                     /locus_tag="P5558_22015"
     CDS             1094..1942
                     /locus_tag="P5558_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27058.1"
                     /translation="MQKALIRANPKLSRFDPLGRIIYFDDFDEGLQGWTSLIGNYEDN
                     LDRMHPGYRQLTTPMLSNLSFWDNGTHGSVDGSYALKMATRPKAGAQNVAIKRLTYVR
                     ACPIQIEFWFTFKPEASAQKLLDTDLRSVGILLDLQNDDYRIMPHLRYLNAFEGQRVE
                     KWQFKEKTVPFERFSEKTVTHYHLAERDWQDLSGERQVLCYNEIPTKVNWQYAKLGFD
                     LRTMRYTHFECNDVAYDMSGIGALQIDAMPNLRGMLNVGFFVESDADRRSSFYVDSIL
                     VSGEWD"
     gene            1942..2283
                     /locus_tag="P5558_22020"
     CDS             1942..2283
                     /locus_tag="P5558_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27059.1"
                     /translation="MPTLRESTTAVVIRGEHVTDGFTSEPYEAGWAIEAVLFLRLLAP
                     PVRPGGMIRLELSADGFNWATDGEPVALPKAEGEVVFRRIGHFGNWLRLRLEMAEGST
                     VQLLATLHLKG"
     gene            2280..3134
                     /locus_tag="P5558_22025"
     CDS             2280..3134
                     /locus_tag="P5558_22025"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRY27060.1"
                     /translation="MTAGQPEGVPFVWSFEGSRPGPHLMIAGLTHGNEPAGRDAVLRL
                     VEKSVRPLAGRLTLALVNPAAAALGRRFVDRDMNRLWRDDWLDEDSSSVEAARARELR
                     PLLRQVDVVLDLHTTTFVARPFFVIADLAKTRALADRMAWPPTQQLMPGGCVDGRHLT
                     DYGRFSDPGHPAVAVTVECGTHDAAASAGVALEAARRFLDVVQGRVPARPNASIERFR
                     TKEPCIALTDRFVLRIPSTGFVAVRQGEVVAMDGGRPVEAPGDLVIVSPRPDPRAGQT
                     AFLWCERC"
     gene            complement(3140..3215)
                     /locus_tag="P5558_22030"
     tRNA            complement(3140..3215)
                     /locus_tag="P5558_22030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3180..3182),aa:Thr,seq:tgt)
     gene            complement(3251..4174)
                     /gene="rlmB"
                     /locus_tag="P5558_22035"
     CDS             complement(3251..4174)
                     /gene="rlmB"
                     /locus_tag="P5558_22035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRY27061.1"
                     /translation="MPPKPPRRDGPGRSGGPERPAGPGRQTRGGDRPTRGPREGQRPG
                     GPRDGDRPGGQRRGPERSRSERPAPDQLWLFGEHAVLAAIRNQRRTCHQLLASEEGMP
                     RISEAAARRGLHVTPCPNPEIAKRLPPGAVHQGLALHVAPLPQLQLERVIAIQPEKSL
                     LLALDQVTDPRNFGAILRSAVAMGVNAVIVPERRSAELSGTAAKAASGALDLVPIVEV
                     VNLSRALARLKEADFRISGLDADGEVTIDAAPPAARRVLVVGSEGSGMRRLVGEHCDE
                     ILSIPIDRRIDSLNVSVATSLALWSLTRAGR"
     gene            4246..4331
                     /locus_tag="P5558_22040"
     tRNA            4246..4331
                     /locus_tag="P5558_22040"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4280..4282,aa:Tyr,seq:gta)
     gene            4367..4440
                     /locus_tag="P5558_22045"
     tRNA            4367..4440
                     /locus_tag="P5558_22045"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4399..4401,aa:Gly,seq:tcc)
BASE COUNT          693 a         1576 c         1525 g          783 t
ORIGIN      
        1 gtggcgttga tgttccgcta cgtcttcgcc tttcgcctct tcgccgaggt ctggctgctc
       61 accggcggcg ggccggcgcg gcagacggag gtgctggcga cctatctcta ccgccacgcc
      121 tttcgctacc aggagttcgg cgtggcctcg gccatcggct ggctcatggt ggtcggcacg
      181 ctgctcatct cggccgtgta tctgcgcgag atgtacaagc gcatgctgag cgccgatgcg
      241 tgaggggccc accaggcgcg ccggcaagct cgcggccctg gcgctgatcg ccatctggtc
      301 ggggctgccg atcctcatcg tcgtgctctc gtcgttcaag ccgccacgcg agatcctggc
      361 cttcccgccg aagctgctct tctcgcccac gttcgagaat tatcgagcgc tcatcgtgcg
      421 ttggcccgag ttcttcgaag cgctccagag cagcctgatc gtcaccaccg gggccacgat
      481 cctcaccgtg gtcgtcacca cgctcgccgg ctacgtctat gccagggtcc ggagccgcaa
      541 gctcgcggcc agcgccttca tgatgatcgt catccgcatg gtgccgccca tcgtcgtcac
      601 cctgccgctc ttcccggtcg tcaactggct tcggctgaac gacacccaca ccgtcctcat
      661 cctgctctat gcgaccttct tcgtctcgct caacacctgg atcatgaagg ccttcatcga
      721 ccagatcccg gtcgagctcg aggaggcggc gatcatcgac ggcgccggcg tctggcagat
      781 cctccgccac gtcatcgtgc cgctcgccgc ccagggcatg atcgcggcct cgaccttcgt
      841 tttgatcttc tcgtggaacg agttcctctt cgccttcatc ttcaccacca ccgaggcgaa
      901 gacggcaccc ttgatcctca gcgagatggt gggtgcggtg gacggggtcg agtggggggt
      961 gctcttcgcc gccgccacgc tgcagctcct gccgatcctg atcttcgtga tcgccgtgca
     1021 gaaatacgtc atcgccggca tgaccgccgg ctcgctcaaa ggctagtcag ccgggaggtc
     1081 gcacttggac gccatgcaga aggccctgat ccgggccaac ccgaagctct cgcgcttcga
     1141 tcccctggga cggatcatct atttcgacga tttcgacgag gggctccagg gctggacctc
     1201 gctcatcggc aattacgagg acaatctcga ccggatgcat ccgggctacc gccagctcac
     1261 cacgccgatg ctctcgaacc tctccttctg ggacaatggc acgcatggca gcgtcgatgg
     1321 cagctatgcc ctgaagatgg cgacccggcc gaaggcgggc gcccagaacg tcgccatcaa
     1381 gcggctcacc tacgtccgtg cctgcccgat ccagatcgag ttctggttca ccttcaagcc
     1441 cgaggcgagt gcccagaagc tgctcgacac ggacctgcgg tccgtcggca tcctgctcga
     1501 cctgcagaac gacgactacc ggatcatgcc gcatctgcgc tacctcaacg ccttcgaggg
     1561 ccagcgggtc gagaagtggc agttcaagga gaagacggtg ccgttcgagc ggttcagcga
     1621 gaagacggtg acccactacc acttggcgga gcgcgactgg caggaccttt ccggcgagcg
     1681 ccaggtgctc tgctacaacg agatcccgac caaggtgaac tggcagtacg cgaagctcgg
     1741 cttcgacctc aggaccatgc gctacaccca tttcgagtgc aacgacgtgg cctacgacat
     1801 gagcggcatc ggcgccttgc agatcgacgc catgcccaac ctgcgcggca tgctgaatgt
     1861 cggcttcttc gtcgaatcgg acgccgaccg gcggtcgtcg ttctacgtcg attcgatcct
     1921 ggtctcgggg gagtgggact gatgccgacc ttgcgcgaga gcacgacggc cgtggtcatc
     1981 cgcggcgagc atgtgacgga cggcttcacc agcgagccct acgaggcagg ctgggcgatc
     2041 gaggcggttc tgttcctgcg gcttctggcc ccgccggtcc ggccgggcgg catgatccgc
     2101 ctcgagctct cggccgacgg tttcaactgg gccactgacg gcgagcccgt cgcgctgccg
     2161 aaggccgagg gcgaggtcgt cttccggcgg atcggccatt tcggcaactg gctccgcctg
     2221 cggctcgaga tggccgaggg cagcaccgtc cagctcctgg cgacgctgca tctgaaggga
     2281 tgacggcggg gcaacccgaa ggcgttccct tcgtctggag cttcgagggc agccgtcccg
     2341 gcccgcatct gatgatcgcc ggcctgaccc acggcaacga gccggcgggt cgcgacgccg
     2401 tcctccggct cgtcgagaag agcgtacgac ccttggctgg ccgcctgacg ctggcgctgg
     2461 tcaacccggc ggccgctgcc ctgggtcggc gcttcgtcga tcgcgacatg aaccgcctct
     2521 ggcgcgacga ctggctggac gaggacagct cgagcgtcga ggcggcacgg gctcgcgagc
     2581 tgcggccgct cctgcgccag gtggacgtgg tgctcgacct gcacacgacg accttcgtcg
     2641 cccggccctt cttcgtcatc gccgatctcg ccaagacccg ggcactcgcc gatcgcatgg
     2701 cctggccgcc gacccagcag ctcatgccgg gcggctgcgt cgacggccgg catctcacgg
     2761 actatggccg gttctccgac cccggtcatc cggccgtcgc ggtcaccgtc gaatgcggca
     2821 cccacgacgc tgcggcgagt gccggggtgg cgctcgaggc cgctcgccgc ttcctcgacg
     2881 tggttcaagg gcgtgttccg gcgcgaccga atgcctcgat cgagcgcttt cgcacgaagg
     2941 agccctgcat cgcgctgacc gaccgcttcg tgctccgcat cccgtcgacg ggcttcgtcg
     3001 cggtgcggca aggcgaggtg gtggccatgg acggcggccg gccggtcgag gcaccgggcg
     3061 acctcgtcat cgtctcgccg cggcccgatc cacgggcggg tcagaccgcg tttctctggt
     3121 gtgagcgctg ctgaagtgct ggtgccggcg gcagggctcg aacccgcgac ctaccgctta
     3181 caaggcggtt gctctaccag ctgagctacg ccggcacgga tcgtggctta gggcctcgac
     3241 gcccccgccg tcaacggccg gcccgcgtca ggctccagag agcgaggctg gtcgccaccg
     3301 agacgttgag gctgtcgatc cgccggtcga tcgggatcga gaggatctcg tcgcaatgct
     3361 cgccgaccag ccggcgcatg cccgagccct ccgagcccac gaccagcacg cggcgggctg
     3421 ccgggggggc ggcatcgatg gtcacctcgc catcggcgtc gagcccgctg atgcggaaat
     3481 cggcctcctt gagccgggcg agggcccgcg agagattgac cacctcgacg atcggcacga
     3541 ggtcgagggc gcccgatgca gccttggcgg ccgtgccgga aagctcggcg ctgcggcgct
     3601 ccggcacgat cacggcgttc acgcccatgg cgacggcgct gcgcaggatg gcgccgaaat
     3661 tgcgcgggtc ggtcacctgg tcgagggcca ggagcaggct cttttcgggc tggatggcga
     3721 tcacccgctc gagctggagc tgcggcaggg gtgctacgtg cagggccagg ccctgatgca
     3781 ccgcaccggg cggcaggcgc ttcgcgatct ccgggttggg gcagggcgtg acgtgcaggc
     3841 ctcgcctggc ggccgcctcg gaaatgcgcg gcatgccctc ctcgctggcg aggagctggt
     3901 ggcaggtgcg ccgctggttc ctgatcgccg ccaggaccgc atgctcgccg aagagccaga
     3961 gctggtccgg ggccgggcgc tcggaccggc tccgctcggg gccgcgtcgc tggccgcccg
     4021 gtcggtcgcc atcgcggggg ccgcccggtc gctggccttc gcgcgggcca cgggtcggtc
     4081 ggtcaccacc acgggtctgg cggccgggtc cggctgggcg ctcggggcca ccggatcggc
     4141 cggggccatc tcgtctggga ggcttgggag gcatgggtcg tccagttgac aagacagtgg
     4201 atcaggcgat accacccggc acgttttcgc ccgaggcgga ctcccggagg ggtggccgag
     4261 tggttaaagg cagcagactg taaatctgcc cgcgtacgcg tacgtaggtt cgaatcctac
     4321 cccctccacc aatccgccac ggtcgtcgac gtcgcttagg tcaagtgcgg gtgtagctca
     4381 acggtagagc tccagccttc caagctggcg gtgggggttc gattcccctc acccgctcca
     4441 tccaccaacg tacccgacgg tccgaagccg gacaacgtgt cggcaacctc acctcgagca
     4501 gggtagaact gggaacccgg tcatggccaa ggaaaagttt cagcgggaca agccgcacgc
     4561 gaacattggt acgattg
//
LOCUS       DAOTFI010000317         4490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_22962_length_4490_cov_6.829763, whole genome shotgun sequence.
ACCESSION   DAOTFI010000317 DAOTFI010000000
VERSION     DAOTFI010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22962_length_4490_cov_6.829763"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="P5558_22050"
     CDS             complement(<1..538)
                     /locus_tag="P5558_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRY27062.1"
                     /translation="MRIVDSHHHLWDRDLHPYPWLAKPVQSPAIGDTTPLCRNYLLAD
                     FLADARGLHLAKSVHLQAEIAPELAVEETRWLQAIADSPGARGFPHAIVAFADLAAPD
                     VDRVLEAHGEFANMRGIRQILNRHSDPALNAASVDYLNDPAWQQGYARLAKHGYSFDL
                     QLYWPQMADAAALAKRHPD"
     gene            complement(551..1735)
                     /locus_tag="P5558_22055"
     CDS             complement(551..1735)
                     /locus_tag="P5558_22055"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY27063.1"
                     /translation="MTTISRVEVHVFGYEVEDLALPPHGAAGVGNLVWQKGGRMPMSR
                     YAVSIETKDGARGEYVTHWVGTPSALGQTLMLAPHLLGRDPDARVEIWEDMKREIRAY
                     DHMGHGPIDIALWDLAGKRLGASVARLLGGYRKRLPTYASTYHGQDPKGGLDTPEAFA
                     DYAAACKEQGFAGFKIHGWHDGDVAREVRNVLGVRAAVGDDFPLMLDPACQLRTWMDA
                     LRVGRACDEAGFFWYEDPYRDAGVSAEGHKRLREKLRTPLLVSEHVRTIEQKAAFLMA
                     GGCDMIHADPEYDMGITGAIKLAHLCEAFGLDLQFHACGPAHRAVISATRNTHFYEMA
                     LVGPGMANAVPPVYACGYSDQPEDLGKDGCVPVPDGPGLGVTYDWAHIEKHRTAHHVF
                     GG"
     gene            complement(1732..2766)
                     /locus_tag="P5558_22060"
     CDS             complement(1732..2766)
                     /locus_tag="P5558_22060"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY27064.1"
                     /translation="MDPFEKRRLGKSGVEISSFGFGGAPLGELFTRVSDADAEATLQA
                     AWDEGIRYYDTAPWYGRGQSEHRLGRFLYRQPREEFVLSTKVGRILKAPRHPERFDTG
                     FWTGGLHFDHVFDYSYDGIMRAYEDCLQRLGMNRIDLLVIHDLDLRHHAADAKVAAYL
                     AQLATTGWRALDELKTAGLIRGIGAGINEEGMIPRFLDLVDLDFFLLALRYTLMDHDT
                     LDVELPYCQRRDVGLVIGAVFSSGITATGPVPGAKYDYADATPEALEKVRRIKAVCER
                     HEVALPAAALQFPFGHPCVASVIPGGFEPAHIRANLDHMRATIPADFWAELRHEKLIR
                     ADAPVPGDNA"
     gene            complement(2785..3744)
                     /locus_tag="P5558_22065"
     CDS             complement(2785..3744)
                     /locus_tag="P5558_22065"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27065.1"
                     /translation="MSDPARRRELLLQSYGLLQALAVLAVLVVVMSILSPRFLSWINI
                     SNLMGQMAVNLIVAAGMTIVMISGEFDISVGSVVALAAAVSAALMQQMGVVPAVLLSL
                     LIGPAIGFFNGVITTKGRIPSFIVTLGTLMMARSLAFVVTGGQVISNLPEEFKAIGQG
                     RLFNVPIPFLIALLCFAVGWFVLARTAFGKRVYAVGANRMVATLSGIHADRVKIQCMM
                     IVGFTAAFAGNILLARIGAIQADTARGLEFEVIAAVVIGGTSLYGGQGNILRTIIGVI
                     IIALIRNFLNLSRIDIFWQDFATGAIIIAAVLLDALQKRVGQR"
     gene            complement(3750..>4490)
                     /locus_tag="P5558_22070"
     CDS             complement(3750..>4490)
                     /locus_tag="P5558_22070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY27066.1"
                     /translation="QDLARMEGEGGGPRDVAFTLHAGEILGVTGLTGSGLDALGKAIV
                     ASPDTRREAGTLRLEGRPLQVATPRDALDAGIVLLTNDRLREGLLPESPIWENVTLPV
                     LGRFAGTGGILDLEAMLAAGRDAIKKLTIRAPGPTTLARALSGGNQQKVLFAKWLATE
                     PRVFIMDEPTIGIDVGSKAEIRAIVRETAAAGVGVLLITAEIDELTSLCDRVLVMFRG
                     RIVADLTGDAIERGAILHASVSGARAAA"
BASE COUNT          698 a         1389 c         1719 g          684 t
ORIGIN      
        1 tgtcgggatg gcgtttcgcc agggcggcgg catcggccat ttgcggccag tagagctgga
       61 ggtcgaagga gtagccgtgc ttcgcgaggc gggcgtagcc ctgttgccag gcggggtcgt
      121 tcaggtagtc gacgctggcg gcgttgaggg cgggatcgct gtggcggttc aggatctggc
      181 ggatgccgcg catgttggcg aactcgccgt gcgcctccag gacccggtcg acgtcggggg
      241 cggcgaggtc ggcgaaggcg acgatggcgt gggggaagcc gcgcgcgccc gggctgtcgg
      301 cgatggcctg cagccagcgg gtctcctcga cggcgagctc gggtgctatc tcggcctgga
      361 ggtggacgga cttcgccaga tggaggcccc gggcatcggc gaggaagtcg gccaggaggt
      421 agtttcggca gagtggggtg gtgtcgccga tcgcgggcga ctggaccggc tttgccagcc
      481 agggataggg gtgcaggtcg cgatcccaga ggtggtggtg ggagtcgacg atgcgcatgc
      541 gctcaaagcc tcaaccgccg aagacgtggt gggcggtgcg gtgcttctcg atatgggccc
      601 agtcataggt gacgccgagg ccggggccgt cggggaccgg gacgcagccg tccttgccca
      661 ggtcctccgg ctggtcgctg tagccgcagg cgtagacggg cgggacggcg ttcgccatgc
      721 cggggccgac cagcgccatc tcgtagaaat gggtgttgcg ggtggcgctg atgacggcgc
      781 ggtgggccgg gccgcaggcg tggaactgga ggtcgaggcc gaaggcctcg cagagatggg
      841 cgagcttgat cgccccggtg atgcccatgt cgtactcggg gtcggcatgg atcatgtcgc
      901 agccgccggc catgaggaag gccgccttct gctcgatggt gcggacatgc tcgctgacca
      961 ggagcggtgt cctgagcttc tccctgaggc gcttgtggcc ctcggcggag accccggcat
     1021 cgcgataggg atcctcgtac cagaagaagc cggcctcgtc gcaggcgcgg ccgacgcgga
     1081 gcgcgtccat ccaggtgcgg agctggcagg cggggtcgag catcaagggg aagtcgtcgc
     1141 cgacggcggc gcgcacgccc agcacgttcc tgacctcgcg ggcgacgtcg ccgtcgtgcc
     1201 agccgtggat cttgaagccg gcgaagccct gctccttgca ggcggcggcg tagtcggcga
     1261 aggcctcggg cgtgtcgagc ccgcccttcg ggtcctggcc gtgataggtg ctggcatagg
     1321 tcggcaggcg cttcctgtag ccgccgagca gccttgccac cgaggcgccg agccgcttgc
     1381 cggcgagatc ccagagcgcg atgtcgatgg gcccgtggcc catgtggtcg taggcccgga
     1441 tctcgcgctt catgtcctcc cagatctcga ccctggcgtc cgggtcgcgg ccgagcagat
     1501 gcggggcgag catcagggtc tggccgagcg cggacggcgt gccgacccag tgagtcacgt
     1561 actcgcccct ggcaccgtcc ttcgtctcga tgctgacggc gtagcggctc atcggcatcc
     1621 gcccgccctt ctgccagacg agattgccga cgccggccgc accgtgcggc ggcagggcca
     1681 gatcctcgac ctcgtagccg aagacgtgga cctcgacgcg cgagatggtg gtcatgcgtt
     1741 gtctccaggg accggtgcgt cggcgcggat cagcttctcg tggcgaagct cggcccagaa
     1801 gtcggccggg atggtagcgc gcatgtggtc gagattggcc ctgatatgcg ccggctcgaa
     1861 gccgcccggg atgaccgagg cgacacacgg atggccgaag gggaactgca gggccgcggc
     1921 cggcagggcg acctcgtgcc gctcgcagac ggcctttatg cgacgcacct tctcgagcgc
     1981 ctcgggcgtg gcgtcggcgt agtcgtactt ggcgcccggg acagggccgg tcgcggtgat
     2041 gccggagctg aagacggcgc cgatgacgag gccgacatcg cgcctctggc agtagggcag
     2101 ctccacgtcc agcgtgtcgt gatccatcag cgtgtagcgc agcgccagga ggaagaagtc
     2161 gagatcgacc agatcgagga agcgcgggat catgccctcc tcgttgatgc cggcgccgat
     2221 gccccggatc agacccgccg tcttgagctc gtcgagcgcc cgccagccgg tggtggcgag
     2281 ctgggccaga taggccgcga ccttggcgtc ggccgcgtgg tggcggagat cgaggtcgtg
     2341 gatgacgagc aggtcgatgc ggttcatgcc gaggcgctgc aggcagtcct cataggcgcg
     2401 catgatgcca tcgtagctgt agtcgaagac gtggtcgaaa tgcagcccgc cggtccagaa
     2461 gccggtgtcg aagcgctccg ggtgccgggg tgccttcagg atgcggccga ccttggtcga
     2521 gaggacgaat tcttcacgcg gctggcggta gaggaagcga cccagccgat gctcgctctg
     2581 gccccggccg taccaggggg ccgtgtcgta gtagcggata ccctcgtccc aggcggcctg
     2641 gagcgtcgcc tcggcgtcgg cgtcgctgac gcgcgtgaag agctcgccca gcggcgcccc
     2701 gccgaagccg aaggacgaga tctcgacccc ggacttgccg aggcggcgct tctcgaacgg
     2761 gtccatgagc gtgtctcccg agtgctagcg ctggccgacg cgcttctgca gggcgtcgag
     2821 caggacggcg gcgatgatga tcgcccctgt ggcgaagtcc tgccagaaga tgtcgatcct
     2881 ggacaggttc aggaagttgc ggatgagcgc gatgatgatg acgccgatga tggtgcgcag
     2941 gatgttgccc tggccgccat agaggctggt gccgccgatg accacggcgg cgatcacctc
     3001 gaactcgagg ccgcgcgccg tgtcggcctg gatggcgccg atgcgggcga gcaggatgtt
     3061 gccggcgaag gcggcggtga agcccacgat catcatgcac tggatcttca cccggtcggc
     3121 gtggatgccg gaaagcgtgg cgaccatgcg gttggcgccg acggcgtaga cccgcttgcc
     3181 gaaggcggtg cgggcgagga cgaaccagcc gacggcgaag cagagaaggg cgatcaggaa
     3241 cgggatcggg acgttgaaga ggcgaccctg gccgatcgcc ttgaactcct ccggcaggtt
     3301 cgagatgacc tggccgccgg tgacgacgaa ggcgagcgag cgcgccatca tcagcgtgcc
     3361 gagcgtgacg atgaaggagg ggatgcggcc cttggtggtg atcacgccgt tgaagaagcc
     3421 gatggccggg ccgatcagga gcgagagcag gaccgccggg acgacgccca tctgctgcat
     3481 gagggcggcc gagacggcgg cggccagcgc cacgaccgag ccgaccgaga tgtcgaactc
     3541 gcccgagatc atgacgatgg tcatgccggc ggcgacgatc aggttgaccg ccatctggcc
     3601 catcaggttc gagatgttga tccaggagag aaagcgcggc gagaggatcg acatgacgac
     3661 gacgaggacg gcgagcaccg ccaacgcctg gagcaggccg taggactgca gcagcagctc
     3721 gcgccgcctg gctggatcgc tcatcatcat catgctgccg cgcgcgctcc gctgaccgag
     3781 gcgtgaagga tggcgccgcg ctcgatggcg tcgccggtga ggtcggccac gatgcggccg
     3841 cggaacatga ccagcacgcg atcgcacaac gaggtgagct cgtcgatctc ggcggtgatg
     3901 agcaggacac cgacccccgc cgccgcggtc tcgcggacga tggcgcggat ctcggccttg
     3961 gagccgacat cgatgccgat ggtcggctcg tccatgatga agaccctggg ctctgtcgcc
     4021 agccacttgg cgaagagcac cttctgctgg ttgccgccgg aaagcgctct cgccagggtg
     4081 gtcgggccgg gggcgcggat ggtgagcttc ttgatggcgt cgcggccggc ggccagcatg
     4141 gcctcgaggt cgaggatgcc gccggttccg gcgaagcggc cgagcaccgg cagggtgacg
     4201 ttctcccaga tcgggctctc gggcagcagg ccctcgcgca gccggtcgtt ggtcaggagc
     4261 acgatgccgg catcgagcgc gtcacgcggg gtcgccacct gcagcggccg gccttcgagg
     4321 cggagcgtgc cggcctcgcg cctggtgtcc gggctcgcga cgatggcctt gccgagcgcg
     4381 tcgaggccgg agccggtgag cccggtgacg cccaggatct cgcccgcatg gagggtgaag
     4441 gcgacgtcgc gcgggccgcc gccttcaccc tccatgcggg cgagatcctg
//
LOCUS       DAOTFI010000318         4450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_23243_length_4450_cov_6.202048, whole genome shotgun sequence.
ACCESSION   DAOTFI010000318 DAOTFI010000000
VERSION     DAOTFI010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23243_length_4450_cov_6.202048"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="P5558_22075"
     CDS             <1..598
                     /locus_tag="P5558_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY27067.1"
                     /translation="GIMTPTEAAAVATVYALFLGLVVYRELDLRELPKLIVSTVEVTG
                     MVLALVMTAAALAWCLSISRIPQTITPIIVEHLGNPLLFLLAVNLLLLFIGCFMEALA
                     AMLILIPILTPVAAQLGIDPIHFGVIFVLNLMLGTITPPVGVVLFITSRLAGISFERM
                     SIAIVPWLIPLLVVLVLLSLWPPLTTFLPGFFLGRPGF"
     gene            647..1609
                     /locus_tag="P5558_22080"
     CDS             647..1609
                     /locus_tag="P5558_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY27068.1"
                     /translation="MADVLCFGDLLIDFVPTESGLPLSRVETFRRAPGGAAANVAVGL
                     GRLGVSSAFMGKVGDDVFGHFLADTLRADGVDVGPLRFDGRARTALAFVSLKADGERD
                     FMFYRHPSADMLFTPGEVDAGAIEAASIFHFDSISLAAEQPRETALFAADRAVAAGKL
                     VSFDVNLRLPLWGDADAARAGIRDGLKRAAVVKLSDDELEFLIGNRDPSGLRDALWHD
                     GIRLLVLSIGRAGCVVFTAEGEFRVPSFEVKAVDTTGAGDGFVAGLLAGLHASPGGFG
                     DRDALFSACRFANAVGALTTTERGAIPALPGRDQVEALVNRSGR"
     gene            1634..3118
                     /gene="zwf"
                     /locus_tag="P5558_22085"
     CDS             1634..3118
                     /gene="zwf"
                     /locus_tag="P5558_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRY27069.1"
                     /translation="MSARLLPVDPFDLVVFGGTGDLAMRKLLPGLYHRDCDGQLPPES
                     RIIGVSRGDLGKKAYAEAVETAIRRSTPEEQLRPEVVDRFLKRLEHVAIDAAGTTGWR
                     KLAALLQRNGKSNGIRVFYLATAPALFGPIARNLAANELITAESRVVLEKPIGRDLDS
                     AMAINDAVGAVFEERQIFRIDHYLGKETVQNLVALRFANALFEPLWNSAHIDHVQITV
                     AESLGVEGRAAYYDTSGALRDMVQNHILQLLCLVAMEPPHVFAAEAVRNEKLKVLRSL
                     RPIDNGDVTTKTVRGQYQAGAVGGQAVPGYLTELDRPASSTETFVVIKAEIANWRWAG
                     VPFYLRTGKRMMTRQSEIVIQFKAPPYSIFGEAGGPLDANRLVVRLQPDEAVRLFLMS
                     KDPGPGGMRLRTVPLNLAYADAFNVRYPDAYERLLLDVVRGNATLFMHKDEVEAAWTW
                     VEPILRAWEARNDPPKPYTAGSWGPSAAIALIERDGRNWLEDAA"
     gene            3130..>4450
                     /gene="edd"
                     /locus_tag="P5558_22090"
     CDS             3130..>4450
                     /gene="edd"
                     /locus_tag="P5558_22090"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HRY27070.1"
                     /translation="MTSELHARLGEVTERLARRSHDRRQRYLDKIRAAASRGPKRGHL
                     SCGNLAHGFAACDAGDKADLMGEAKANIAIVSSYNDMLSAHQPLERFPALIKAACREA
                     GAVAQFAGGVPAMCDGVTQGQPGMELSLFSRDVIAMATAVALSHDMFDGALYLGVCDK
                     IVPGLVIAALSFGHLPAVFVPAGPMPSGLPNDEKAKVRQLFAEGKVGRDRLLESEAAS
                     YHAPGTCTFYGTANTNQMLMEIMGVHLPGASFVNPNTPLRDALTSHAARRVTEITAMG
                     NAYTPIGEVMDEKAFVNGVVGLHATGGSTNHTLHLVAMARTAGIDLTWDDMADLSEVV
                     PLLCRIYPNGLADVNHFQAAGGMGYLTRELLGAGLLHADVRTVAGEGGLDLYAKEPFL
                     EEGRLAWRDGPSVSGDASVLATVEKPFSRDGGLKLLEGNLGRAVIKVS"
BASE COUNT          634 a         1609 c         1485 g          722 t
ORIGIN      
        1 cggcatcatg acgccgaccg aggcggcggc ggtcgccacg gtctatgccc tcttcctcgg
       61 gctcgtggtc tatcgcgagc tcgatctgcg cgagctgccg aagctcatcg tctcgacggt
      121 cgaggtgacc ggcatggtgc tcgcgctggt catgacggcg gcagcactcg cctggtgcct
      181 ctcgatctcg cgcatcccgc agaccatcac gccgatcatc gtcgagcatc tcggcaaccc
      241 gctgctcttc ctcctcgcgg tcaacctgct gctgctgttc atcggctgct tcatggaggc
      301 gctcgccgcc atgctgatcc tgatcccgat cctcacaccg gtcgcggccc agctcggcat
      361 cgacccgatc catttcggcg tgatcttcgt cctcaacctg atgctgggga ccatcacccc
      421 gccggtcggg gtggtgctct tcatcacctc gcggctggcg ggcatcagct tcgagcgcat
      481 gtcgatcgcc atcgtgccct ggctgatccc gctgctcgtc gtgctggtgc tgctgtcgct
      541 ctggccgccc ttgacgacct tcctcccggg gttctttctc ggccggccgg gcttttgacc
      601 ggcggttcct cccgatgcgg gcgggcggcg aaggaagggt gcgaccatgg ctgatgtcct
      661 ctgcttcggc gatcttctaa tcgatttcgt gccgaccgaa agcggcctgc cgctgagtag
      721 ggtcgagacc tttcgccggg cgcccggcgg ggcggcggcc aacgtcgccg tcgggctcgg
      781 ccggctcggg gtttcgagcg ccttcatggg caaggtcggc gacgacgtct tcggccattt
      841 cctcgccgac acgctgcgcg ccgacggggt cgatgtgggg ccgctccgct tcgacggtcg
      901 ggcccgcacg gcgctcgcct tcgtcagcct caaggcggat ggcgagcggg atttcatgtt
      961 ctaccgccac ccctcggccg acatgctgtt cacgccgggc gaggtggacg cgggtgcgat
     1021 cgaggcggcc tcgatcttcc acttcgactc catcagcctc gccgccgagc agccacgcga
     1081 gacggcgctc ttcgccgccg atcgggcggt cgcggccggc aagctggtga gcttcgacgt
     1141 caacctgcgg ctgccgctct ggggcgacgc cgacgccgcc cgggccggca tccgcgacgg
     1201 gctgaaacgg gccgccgtcg tcaagctgag cgacgacgag ctcgagttcc tgatcggcaa
     1261 ccgcgatccc tcgggcctgc gcgacgccct ctggcacgac ggcatccggc tgctcgtgct
     1321 cagcatcggc cgtgccggct gcgtcgtctt cacggccgag ggcgagttcc gcgtgccgag
     1381 cttcgaggtg aaggcggtcg acacgaccgg cgccggcgac ggcttcgtcg ccggcctgct
     1441 cgccggcctg catgccagcc cgggcggctt cggcgaccgc gacgccctct tctccgcctg
     1501 ccgcttcgcc aacgcggtgg gtgccctcac gacaaccgag cggggggcga ttccggccct
     1561 ccccggacgc gatcaggtcg aggcgctcgt gaaccggtcc ggccgatgag ccgggccaag
     1621 tggagttcaa aggatgagcg cacgtctcct tccggtcgac ccgttcgacc tcgtggtgtt
     1681 cggcggcacg ggcgacctcg ccatgcgcaa gctgctgccg ggcctttatc atcgtgactg
     1741 cgacggccag ttgccgccgg agagccggat catcggcgta tcgcgcggcg acctcggcaa
     1801 gaaggcctat gcggaggcgg tggagacggc gatccggcgc tcgaccccgg aagagcagct
     1861 tcgccccgag gtcgtcgacc gcttcctgaa gcggctggag catgtcgcca tcgatgccgc
     1921 cggcaccacc ggctggcgca agctcgcggc gctcctgcag cgcaacggca agagcaacgg
     1981 catccgggtc ttctacctcg ccaccgcgcc ggcgctgttc ggcccgatcg cccgcaacct
     2041 cgccgccaac gagctgatca cggccgagag ccgcgtcgtg ctcgagaagc cgatcggtcg
     2101 cgatctcgac tccgccatgg cgatcaacga tgcggtgggg gcggtcttcg aggagcggca
     2161 gatcttccgc atcgaccatt atctgggcaa ggagacggtc cagaacctgg tggccttgcg
     2221 cttcgccaac gcgcttttcg agcccttgtg gaactcggcg cacatcgacc atgtgcagat
     2281 caccgtcgcc gagagcctcg gcgtcgaggg ccgggccgcc tattacgaca cctcgggcgc
     2341 gctccgcgac atggtgcaga accacattct ccagctcctc tgcctcgtcg ccatggagcc
     2401 gccgcacgtc ttcgccgcgg aggcggtgcg caacgagaag ctcaaggtgc tccgctcgct
     2461 caggccgatc gacaacggcg acgtcaccac caagacggtg cgcggccagt accaggcggg
     2521 tgccgtcggc gggcaggccg tgcccggcta cctcacggag ctcgaccggc cggcctcgtc
     2581 gaccgagacc ttcgtcgtca tcaaggccga gatcgccaac tggcgctggg ccggggtgcc
     2641 cttctatctc aggaccggca agcgcatgat gacgcgccag tcggagatcg tgatccagtt
     2701 caaggcgccg ccctactcga tcttcggcga ggccggcggt ccgctcgatg ccaaccggct
     2761 ggtcgtccgc ctgcagccgg acgaggcggt gcgtctcttc ctcatgtcca aggacccggg
     2821 cccgggcggc atgcgcctgc gcaccgtgcc gctcaacctc gcctatgccg acgcgttcaa
     2881 cgtccgctat cccgatgcct atgagcgcct gctcctggac gtggtgcgcg gcaatgccac
     2941 gctcttcatg cacaaggacg aggtggaggc cgcctggacc tgggtcgagc cgatcctgcg
     3001 cgcctgggag gcccgcaacg acccgcccaa gccctacacc gccggaagct ggggcccgtc
     3061 cgccgcgatc gcgctgatcg agcgcgacgg gcgcaactgg ctcgaggacg ccgcctgaag
     3121 gagaagagga tgaccagcga actccatgcg cggctcggcg aggtcaccga gcgcctcgcc
     3181 cgccgcagcc acgaccgccg ccagcgctat ctggacaaga tcagggccgc cgccagccgc
     3241 ggcccgaagc gcggccatct ttcgtgcggc aacctcgccc acggcttcgc cgcctgcgac
     3301 gcgggcgaca aggccgacct gatgggcgag gccaaggcca atatcgccat cgtctcgtcc
     3361 tacaacgaca tgctctcggc gcatcagccg ctcgagcgct tcccggcgct gatcaaggcc
     3421 gcctgtcgcg aggccggtgc ggtggcgcag ttcgccggcg gcgtgccggc gatgtgcgac
     3481 ggggtgaccc agggccagcc cggcatggag ctctcgctct tcagccgtga cgtcatcgcc
     3541 atggcgaccg ccgtggcgct gagccacgac atgttcgacg gcgccctcta tctcggcgtc
     3601 tgcgacaaga tcgtgccggg gctggtcatc gccgccctat ccttcggtca cctgccggcc
     3661 gtcttcgtgc cggcggggcc gatgccgtcg ggcctgccca acgacgagaa ggcgaaggtc
     3721 cgccagctct tcgccgaggg caaggtcggc cgcgaccggc tgctcgagtc cgaggccgcc
     3781 tcctaccacg cgccgggcac ctgcaccttc tacggcacgg ccaacaccaa ccagatgctc
     3841 atggagatca tgggcgtgca tctgccgggg gccagcttcg tcaaccccaa cacgccgctc
     3901 cgcgatgccc tgaccagcca cgccgcgcgg cgggtgaccg agatcaccgc catgggcaac
     3961 gcctacacgc cgatcggcga ggtgatggac gagaaggcct tcgtcaacgg cgtcgtcggg
     4021 ctgcatgcga cgggcgggtc gaccaaccac acgctgcatc tggtcgccat ggcccggacc
     4081 gccggcatcg acctcacctg ggacgacatg gccgaccttt ccgaggtcgt gcccctgctc
     4141 tgccgcatct acccgaacgg cctcgccgac gtgaaccatt tccaggccgc cggcggcatg
     4201 ggctacctga cgcgcgagct gctcggcgcc ggcctgctgc atgccgacgt gcgcacggtg
     4261 gcgggcgagg gcgggctcga cctctatgcg aaggagccct tcctcgagga gggtcgcctc
     4321 gcctggcggg acggcccttc cgtctcgggc gacgcctcgg tcctggcgac ggtggagaag
     4381 ccgttcagcc gggatggcgg gctcaagctg ctcgagggca atctcggtcg ggcggtgatc
     4441 aaggtctcgg
//
LOCUS       DAOTFI010000319         4405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_23528_length_4405_cov_4.274253, whole genome shotgun sequence.
ACCESSION   DAOTFI010000319 DAOTFI010000000
VERSION     DAOTFI010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23528_length_4405_cov_4.274253"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1373
                     /locus_tag="P5558_22095"
     CDS             96..1373
                     /locus_tag="P5558_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27071.1"
                     /translation="MIAIDLGYRPRPHQQEAHAALQRFSVLVAHRRFGKTVFGVMEAV
                     DRALRCNLARPKTAYIAPTAKQAKRIAWDYLAAYTKPIPGAVMVKGESRAVLPGDRSI
                     YVLGADNPDSLRGDYWDHVTLDEVAQFKPGAWSQSLRPALADRMGSMLAIGTPKGENE
                     LFRLFEQSKTDPEWRGLMYPASATGVIDPAELERLRGEMDAAEFAQEFECSFTAAIVG
                     AYYARELDRAEAEDRLAVVPLDPAHLVHTAWDLGFDDTTAIWFFQLVGLQVRLVDYYE
                     AFGVGLDHYAAELGRKGYAYGTHLLPHDVEQTEIGTGRTRLETLDTLGIRATPVERMP
                     VVDGINAVRTILPRCVFDRQRCAAGLRALRHYRREWRMKSQIFAPAPVHDWASHPADA
                     LRTLAIGLPRTAGRELGQVSGPTIAHHSYSMLD"
     gene            1390..1599
                     /locus_tag="P5558_22100"
     CDS             1390..1599
                     /locus_tag="P5558_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27072.1"
                     /translation="MGGLLSAPKPKPMQKAPAEPVVDEAKILKEQQDQDRLRTGRAAT
                     MLTGETGTGGKMGAVGTRMLIGGGS"
     gene            1586..1879
                     /locus_tag="P5558_22105"
     CDS             1586..1879
                     /locus_tag="P5558_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27073.1"
                     /translation="MAAPDMENQHRKIAGYRELTGAELADIAELKKIEASLCEMCDGL
                     AERARTMSAKRWAALARTNLETGMMFAVKTVAQPSQSVGTFLADRPAAPEQPR"
     gene            1876..3516
                     /locus_tag="P5558_22110"
     CDS             1876..3516
                     /locus_tag="P5558_22110"
                     /inference="COORDINATES: protein motif:HMM:NF023657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HRY27074.1"
                     /translation="MNGARPAAGSESEALDLIRWFERLASARQGEQEPVWEEIAERVL
                     PRQQFTGQRRLANSRDLIFDSTAPLALDRFAAAMESMLTPRGSTWHGLKPRRPELAER
                     PTVSRYLDQVTKTLFALRSASRANFYGNLHEHYLSLGAFGTGAVSAMDEMGRALVYRA
                     IPLADLFIDEDASGRVDAVFRRFSLTARQAIGEYGEDAPPKCREAVDRQPDRRFAFLH
                     CVVPNDAARPGRAGPDGMPWRAVHVSVDDRLTIRAGGFRTMPYAVSRYVTGPGELYGR
                     SPAFQSLADIKMVNRMSRTTINQANRATEPPLLATDDTLPPPLMIPNAVNAGWLDRNG
                     NPRIRPFETGARFEIAFEVLEQRRRSINDAFLVSLFQILVETPSMTATEAMLRAQEKG
                     ALLAPTMGRQQAELVGPLIQRELDIAEAAGLLPPMPRELVDDGGFVEVDYLSPMARMQ
                     RNEEATGLLRTLEAAGPLAEIDPSVLEHIDAAKALRVLARANGVESLLRDDKEAAARI
                     AQRLEQQQAQQMAAAAPGVARATRDIAEAGAVSQEARP"
     gene            3546..4280
                     /locus_tag="P5558_22115"
     CDS             3546..4280
                     /locus_tag="P5558_22115"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRY27075.1"
                     /translation="MIQGDALDVLERLDPGSHGALFTDPPYSSGGQFRGDRMAAPSTK
                     YQKSSARGSHPEFMGDNRDQRSFAVWSERWLRLAWRALEPGALAVIFTDWRQLPTLTD
                     AIQVAGFTWRGILVWDKSLSARPQPGRFRAQAEYMVWASKGPLPPAKGKPPGPGVFRL
                     PVCHQRKIHIADKPVALCRDVLPLLTGPILDPFAGAGPIGLAAIEAGKGYLGIEADPY
                     WHRVASGRLAEAAGENGAGGSWSDAA"
BASE COUNT          686 a         1666 c         1467 g          586 t
ORIGIN      
        1 gatgaacggc aagccgctgg atctgtcgct caagcgcgac accgcgcgca cccggcgacg
       61 cgcctgatcg cccctgttct cagggccgag gccgcgtgat cgcgatcgac ctcggctatc
      121 gcccgcggcc gcaccagcag gaggcccacg ccgccctgca acgcttctcg gtgctggtgg
      181 cacaccgccg cttcggcaag acggtgttcg gcgtgatgga ggccgtcgac cgggcgctgc
      241 gctgcaacct ggcgcggccc aagaccgcct atatcgcgcc gaccgccaag caggcgaagc
      301 gcatcgcgtg ggattacctg gccgcctaca ccaagcccat tcccggcgcc gtcatggtca
      361 agggcgagag ccgcgccgtg ctccccggcg accggtcgat ctacgtgctc ggcgccgaca
      421 acccggacag cttgcgcggc gactattggg atcacgtcac cctcgacgag gtggcgcagt
      481 tcaagcccgg cgcctggtcg caatcgctcc ggccggccct cgccgaccgc atgggctcga
      541 tgctggcgat cggcacgccg aagggcgaga acgagctttt ccggctgttc gagcagagca
      601 agaccgaccc cgaatggcgc ggcctgatgt acccggcgag cgccaccggc gtcatcgacc
      661 cggccgagct cgagcggctc aggggcgaga tggacgcggc ggagttcgcc caggaattcg
      721 agtgctcttt caccgccgcg atcgtcggcg cctactacgc ccgcgagctc gaccgggccg
      781 aggccgaaga ccggctcgcc gtcgtcccgc tcgaccctgc ccacctggtt catacggcgt
      841 gggatctggg cttcgacgac accacggcca tctggttttt ccagctcgtc gggctccagg
      901 tccggctcgt cgactattac gaggcgttcg gcgtcgggct cgaccactac gcggcggagc
      961 tcggccgcaa gggctacgcc tacggcacgc atctgctccc gcatgacgtg gagcagaccg
     1021 agatcggcac cggccggacc cgcctcgaga cgctcgacac gctcggcatc cgcgccaccc
     1081 ccgtggagcg gatgccggtg gtcgacggca tcaacgcggt gcggaccatc ctgccccgtt
     1141 gcgtgttcga ccggcagcgt tgcgccgccg ggcttcgggc gttgcgccac taccgccgcg
     1201 agtggcggat gaagagccag attttcgcac ccgcacccgt ccacgattgg gcgagccatc
     1261 cggccgacgc gctccgcacg ctcgccatcg ggctccccag gaccgccggc cgcgagctcg
     1321 gccaggtcag cggcccgacc atcgcgcacc attcctattc gatgctggac tgaagaggag
     1381 gccccgaaca tgggcggact gttgagcgca cccaagccga agccgatgca gaaggccccg
     1441 gcggagcccg tcgtcgacga ggcgaagatc ctgaaggagc agcaggacca ggaccggctc
     1501 cgcacgggcc gcgccgccac catgctcacc ggcgagaccg gcaccggcgg caagatgggc
     1561 gccgtcggca cgcggatgct gatcggtggc ggctcctgac atggagaacc agcaccggaa
     1621 gattgccggc tatcgcgagc tgaccggcgc cgagctcgcc gacatcgccg agctgaagaa
     1681 gatcgaggcc agcctttgcg agatgtgcga cggcctcgcc gagcgagccc gcaccatgag
     1741 cgccaagcgc tgggcggccc tcgcccgcac caatctcgaa accggcatga tgttcgccgt
     1801 gaagacggtg gcgcagccga gccagagcgt cggcaccttc ctcgccgacc gaccggcagc
     1861 gccggagcag ccgcgatgaa cggcgcccgc ccggccgccg gcagcgagag cgaggccctc
     1921 gacctgatcc gctggttcga gcgcctggca tccgcccgcc agggcgagca ggaaccggtc
     1981 tgggaggaga tcgccgagcg cgttctgccg cgccagcagt tcaccggcca gcgccggctt
     2041 gccaactccc gcgacctgat cttcgactcg acggcacccc tcgccctcga ccgcttcgcc
     2101 gccgcgatgg aatcgatgct cacccccaga ggctcgacct ggcacggcct gaagccccgc
     2161 cggcccgagc tcgccgagcg gccgaccgtc agccgctacc tcgaccaggt gacgaaaacc
     2221 ctcttcgcgc tgcgatcggc gagccgggcg aacttctacg gcaacctgca cgagcactat
     2281 ctctcgctcg gcgccttcgg caccggcgcc gtcagcgcca tggacgagat gggccgcgca
     2341 ctggtctatc gcgccatccc gctcgccgac ctcttcatcg acgaggatgc gagcggccgc
     2401 gtcgacgccg tgttccggcg gttcagcctg accgcccgcc aggccatcgg cgagtatggc
     2461 gaggacgccc cgccgaaatg ccgcgaggcc gtcgatcgcc agcccgaccg ccgcttcgcc
     2521 ttcctccact gcgttgtccc gaacgacgcc gcccgccccg gccgcgccgg gcccgacggc
     2581 atgccgtggc gcgccgtgca cgtcagcgtc gacgaccggc tcaccatccg cgccggcggc
     2641 ttccgcacga tgccctacgc ggtgagccgc tacgtgaccg gcccgggcga gctctatggc
     2701 cggagcccgg ctttccagag cctcgccgac atcaagatgg tcaaccgcat gagccggacg
     2761 accatcaacc aggccaaccg cgcgaccgag ccgccgttgc tcgccaccga cgacacgctc
     2821 ccgccgccct tgatgatccc caacgccgtc aatgccggct ggctcgaccg caacggcaac
     2881 ccccggatca ggccgttcga gacgggtgcc cggttcgaga tcgcgttcga ggtgctcgag
     2941 cagcgccgcc ggtcgatcaa cgatgcgttc ctcgtgagcc tctttcagat cctcgtggaa
     3001 accccgagca tgaccgcgac cgaggccatg ctcagggccc aggagaaagg tgccctcctg
     3061 gcgccgacca tgggccggca gcaggccgag ctcgtcgggc cgctgatcca gcgcgagctc
     3121 gacattgccg aggccgccgg cctcttgccg ccgatgccgc gcgagctggt cgacgacggc
     3181 ggcttcgtcg aggtcgatta cctctcgccg atggcgcgga tgcagaggaa cgaggaggcg
     3241 accggcctgc tccgcacgct cgaggccgcc ggcccgctcg ccgagatcga cccgagcgtg
     3301 ctcgagcaca tcgacgccgc caaggcgctg cgcgtgctgg ccagggcgaa cggcgtggag
     3361 tcgcttctgc gcgacgacaa ggaggccgcc gcccggatcg cgcagcggct cgagcagcag
     3421 caggcgcagc agatggcggc cgccgcaccc ggcgtcgccc gggcgacccg cgacattgcc
     3481 gaggccggcg ccgtcagcca ggaggcgcgg ccgtgaccgc gcccgaacgg aaggccggat
     3541 ggtgcatgat ccagggcgac gccctcgacg tgctcgagcg gctcgacccc ggcagccatg
     3601 gcgcgctctt caccgatccg ccctattcgt ccggcggaca gtttcgcggc gaccgcatgg
     3661 cagcaccctc gaccaagtac cagaagagca gcgcccgcgg cagtcacccg gaattcatgg
     3721 gcgacaatcg cgaccagcgc agctttgcgg tttggtccga acgctggctc cgcctcgcct
     3781 ggcgggcatt ggagcccggc gccctcgccg tgatcttcac cgactggcgc cagcttccga
     3841 cgctcaccga cgccatccag gtcgccggct tcacctggcg cgggattctc gtgtgggaca
     3901 agagcctttc ggcgcggccg cagcccggcc gcttccgggc gcaggcggaa tacatggtct
     3961 gggcatcgaa aggcccgctc ccgccggcga agggcaagcc ccccggcccc ggcgtgttcc
     4021 gcctgcccgt ctgtcaccag cgcaagatcc acatcgccga caagccggtg gccttgtgcc
     4081 gggacgtgct gccgctgctg accggcccga tcctcgaccc cttcgccggc gccggcccga
     4141 tcggcctcgc cgcgatcgag gccggcaagg gctatctcgg gatcgaggcc gacccctatt
     4201 ggcatcgcgt cgccagcggc cggctcgcag aagccgccgg ggagaatggg gccggcggct
     4261 cgtggagcga cgccgcatga gctcgacccg cctcgaggcg ctccggctcg cctggcgcca
     4321 caccttcctg gacggcaccg gccacccgca cgactcggcc gcgatggtgc tggccgacat
     4381 gcaacggctg gcgctgccgg gcgac
//
LOCUS       DAOTFI010000320         4390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_23637_length_4390_cov_6.732411, whole genome shotgun sequence.
ACCESSION   DAOTFI010000320 DAOTFI010000000
VERSION     DAOTFI010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23637_length_4390_cov_6.732411"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="P5558_22120"
     CDS             <1..548
                     /locus_tag="P5558_22120"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRY27076.1"
                     /translation="TEADIGRPKVEAAAARLAAIAPACRVEQHGIRLDGEAAGELVAG
                     YDVIVDGTDNAPTRRLVHDAAMRARRPLVSASVQGVDGQLTLYRAFAGPPHPCLHCLF
                     GETEDDAMLPSCAQGGVLGPAAGLVGCLQAVAVVKLLLDLGPDLSGTLLLVEALGPAI
                     ERLSFARRPECAERCAHHAGW"
     gene            686..1102
                     /gene="dksA"
                     /locus_tag="P5558_22125"
     CDS             686..1102
                     /gene="dksA"
                     /locus_tag="P5558_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRY27077.1"
                     /translation="MADTLDVNYRPTEDEPYMNPRQVEYFRRKLLAWRDELVRGSGLT
                     LKQLQADDSRLSDASDWASAEVQRGLELRTRDRERKLLSKIEAALRRIEDGSYGYCEE
                     TQEPIGLKRLDARPIATLSIEAQERHERRERSYRDD"
     gene            1894..2451
                     /locus_tag="P5558_22130"
     CDS             1894..2451
                     /locus_tag="P5558_22130"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRY27078.1"
                     /translation="MQGAAGLVDDYRIADDSLAWRGLLAVDGPAAFVGAPLDAAGSAE
                     APLAASAVRQLALAIGQAVSGDVRQLSVQLSPEELGGLEITVDFADERRVAVTILAER
                     PETLDLLRGETRQLERLLGQQGVSLAAGGFELGLMADGRGQRREHAGAGPGQGASIDL
                     ADTQDAATATTAAPPLRRGLLNLSV"
     gene            2472..3134
                     /locus_tag="P5558_22135"
     CDS             2472..3134
                     /locus_tag="P5558_22135"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRY27079.1"
                     /translation="MTVDATRASAGTTAAARSGVKLAADFDSFLKLLTTQLTTQDPLS
                     PMDAEQFTSQLVQFSGVEQAIQTNTKLEMLIGLMQANQTTAALQYIGATVELDTSAIA
                     LAAGGTAEIAYELPETAAAVLVEIQDGSGRLVRQLTGPAAAGPRSLVWDGRDGAGAAQ
                     PAGTYSVAVAAVDPLGREIPVPLDTVGRVEALTQTTAGLRLTVDGRTYPVESVRSVRP
                     AA"
     gene            3082..>4390
                     /locus_tag="P5558_22140"
     CDS             3082..>4390
                     /locus_tag="P5558_22140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRY27080.1"
                     /translation="MAEHIPWKACGPCDPPPDIPDTTRRWSEAMSLFGSLYSGVSGLG
                     AQSRAMSMISDNVANVNTTGYKGAVAQFANMVTSKESVSSYSPGGVRSIGAMSISRQG
                     LIQSSNSPTDAAISGAGFFVMNSLPDGTGEQIYTRAGSFSPDFLGDLRSPSGFYLQGW
                     LLDADENVVDVNQTETVNVGNVNGLATATTNISLGANLDADQALHAGVYAAGDIAAGT
                     VDAQFSRNVQVYDSLGRPQPVTMAFLKTAANTWQVELIAADPTSVDALAHPNGLLGTG
                     TVTFNGDGSLATNTLDGATVNVTWAAATGADPSALTLDLGSAGETNGLTQFASPTNVA
                     FVQQNGAEVGELNGVRIDEDGYVIASFTNGENRRLYQLPIATFANPSGLDPRSGNVFA
                     QTEASGAFNLRAAGRGGAGILAPSALEAANVDLADEFTKMIVTQ"
BASE COUNT          624 a         1630 c         1525 g          611 t
ORIGIN      
        1 acaccgaggc ggatatcggc cggcccaagg tcgaggcggc ggcggcccgg ctcgccgcca
       61 tcgccccggc ctgccgcgtg gagcagcacg gcatccggct cgacggcgag gcggcgggcg
      121 agctcgtcgc cggctacgac gtcatcgtcg acggcaccga caacgcgccc acccgtcgcc
      181 tcgtgcacga tgccgccatg cgcgcgcgtc gcccgctcgt ctccgcctcg gtgcagggcg
      241 tcgacggcca gctcaccctc tatcgggcgt tcgccggccc gccgcatccc tgcctgcact
      301 gcctgttcgg cgagaccgag gacgatgcca tgctgccgag ctgcgcccag ggtggcgtgc
      361 tcggcccggc ggcgggcctc gtcggctgcc tgcaggcggt cgccgtggtc aagctcctgc
      421 tcgatctcgg ccccgatctc tcgggcacgc tgctgctggt cgaggcgctc gggcccgcca
      481 tcgagcgcct gtcgttcgcc cgtcgcccgg aatgcgccga gcgctgcgcg caccacgcgg
      541 gctggtaatc cacgagcctg ccaaaagcga ttgtcgcaca gcggcttagg cagggcacat
      601 gaggcccggt tacggttgcg ttcggccgcg tccttgctgt atgggagcgc cgttcgacag
      661 gccttggcat tccctggggg ctcgcatggc ggatacgctc gacgtcaact accgaccgac
      721 cgaggacgag ccttacatga acccgcgcca ggtcgagtat ttccggcgca agctcctggc
      781 ctggcgcgac gagctggtgc gcggctccgg cctgacgctg aagcagctcc aggcggacga
      841 cagccggctc tccgacgcca gcgactgggc gagcgccgag gtgcagcgtg gcctggagct
      901 ccgcacccgc gaccgggagc gcaagctgct cagcaagatc gaggcggcgc tccggcggat
      961 cgaggacggc tcctacggct actgcgagga gacgcaggag ccgatcggcc tgaagcggct
     1021 cgacgcgcgg ccgatcgcga cgctcagcat cgaggcccag gagcgccacg agcggcgcga
     1081 gcgcagctac cgggacgact gagacccggc aagatcggca ggggcccggc aggatttgcc
     1141 gggtgccgcc cccaggggcc ggcccgcccc acctccgtgt cgtggccgat tgcggcggat
     1201 cgggatgccc gggcggtttg gcacggcggt tgctgatgct catccgccat gctttcgatc
     1261 aatcctcctt ccggcccgcc ggccgggccc gccacgaccg ccggtgccgc gcgccgggcg
     1321 ggtgacgacg gctccctgcc ggaaggcttc gaggggctgc tggccggcct actggcgccg
     1381 cccgcaccac cgcccgccac cctgccggcg gtcggcctcg cggctgcccc ggcagcgccg
     1441 cctcctgcag aagtcccggt tgccgccccg gtagaaggcg aggacacggt ttctggcgac
     1501 gccgacacgg cgtcccaggg ccgccggccc ggcccgaccc ccgacttcgg gttgccggcg
     1561 acggcgcctg acgcagcacc ggcgaccgcc ccgaccgaag gggcgccgga cgtcgatgtc
     1621 gccggcccgc cacaggggca agccgcgaaa gccccggctc cgggtcctgc acagccgctc
     1681 ccggcggcgg tcgcgccgcg agatgccccg ggcgaccctc cgccaatggc gacggccccc
     1741 gccgttcgtg ccgcccgggc cgggagcgcc ggtgctgctg ccgtcgagcc gcctgccggc
     1801 cgcgccggtg ccgagggggc ggggctgtgg gccgcggtcg gtcccgccgt cgcccggccg
     1861 acgggtcggg ccggcagccc ccagcctggg cccgtgcaag gcgcggctgg cctggtcgac
     1921 gactaccgga tcgccgacga cagcctcgcc tggcgcggcc tgctggccgt ggacgggcct
     1981 gccgccttcg tcggcgcgcc gctcgatgcc gccggctcgg ccgaggcgcc gctggctgct
     2041 tccgccgtcc gccagctcgc tctggcgatc gggcaggcgg tgagcggcga tgtccgccag
     2101 ctctccgtcc agctcagccc cgaggagctc ggcggcctcg agatcacggt cgacttcgcc
     2161 gacgagcgac gggtggcggt gacgatcctc gccgagcgcc ccgagaccct ggacctgctg
     2221 cggggcgaga cccgtcagct cgagcggttg ctcggccagc agggggtttc gctcgcggct
     2281 ggcgggttcg agctgggtct catggccgat ggccgcggcc agcgtcgcga gcatgccggt
     2341 gccgggccgg gccagggcgc ttcgatcgac ctcgcggata cgcaggatgc cgccaccgcc
     2401 accaccgccg cgccgccgct gcggcggggc ctcttgaacc tgtccgtctg attgtccgga
     2461 gtacccaggg aatgaccgtc gatgccacac gagcttcagc cggcaccacc gccgccgcgc
     2521 ggtcgggggt caagctggct gccgatttcg acagctttct caagctcctg acgacccagc
     2581 tgacgaccca ggacccgctg agcccgatgg acgcggagca gttcacctcc cagctggtgc
     2641 agttctccgg cgtcgagcag gcgatccaga ccaacaccaa gctggaaatg ctgatcggcc
     2701 tgatgcaggc gaaccagacg accgcggccc tccagtatat cggcgccacc gtggagctcg
     2761 acacctcggc catcgcgctg gccgccggcg ggacggcgga gatcgcctac gagctgccgg
     2821 agacggcggc ggccgtcctc gtggagatcc aggacgggtc gggcaggctg gtgcgccagc
     2881 tcaccgggcc cgccgccgcc ggtccccgga gcctcgtctg ggacggtcgc gacggagcgg
     2941 gcgccgccca gccggccggg acctacagcg tggcggtcgc cgcggtcgac ccgctcggcc
     3001 gcgagatccc ggtcccgctc gacacggtcg ggcgggtcga ggccctgacc cagacgacgg
     3061 cggggctgcg cctcaccgtc gatggccgaa catatcccgt ggaaagcgtg cggtccgtgc
     3121 gacccgccgc ctgacatccc agacacaaca agacgctgga gcgaagcaat gagcctcttc
     3181 gggtcacttt attcgggtgt ttccggcctc ggcgctcagt cgcgggccat gagcatgatc
     3241 tcggacaacg tcgcgaacgt gaacacgaca ggttacaagg gcgcggtcgc ccagttcgcg
     3301 aacatggtca cgagcaagga atcggtgtcc tcgtactcgc cgggcggcgt gcgctcgatc
     3361 ggtgccatgt ccatcagccg gcagggcctg atccagagca gcaactcgcc caccgacgcg
     3421 gccatctccg gcgcgggctt cttcgtcatg aactccctcc ccgacggcac cggcgagcag
     3481 atctacacgc gcgccgggtc cttctcgccg gacttcctcg gcgacctccg ttcgccgtcg
     3541 ggcttctacc tgcagggctg gctcctcgat gccgacgaga acgtcgtcga cgtcaaccag
     3601 accgagaccg tcaatgtggg caatgtcaac ggcctggcga cggcgacgac gaacatcagc
     3661 ctcggcgcca atctcgacgc cgaccaggcc ctgcatgcgg gcgtctatgc cgctggtgac
     3721 atcgccgctg gcaccgtcga tgcccagttc tcgcgcaatg ttcaggtcta tgacagcctc
     3781 ggccgaccgc aaccggtcac catggcattc ctgaagacgg ctgccaatac ctggcaggtc
     3841 gagctgatcg ccgccgatcc gacgagcgtc gatgcgctgg cgcatcccaa tggcctgctg
     3901 ggcaccggca ccgtcacgtt caacggtgac ggcagcctgg ccacgaacac gctcgatggc
     3961 gccaccgtca acgtcacctg ggcggccgcg acgggcgccg atccgagcgc cttgacgctc
     4021 gacctcggca gcgccggcga gaccaacggc ctcacgcagt tcgcgagccc gaccaacgtg
     4081 gcgttcgtcc agcaaaatgg tgccgaggtc ggcgagctca acggcgtgag gatcgacgag
     4141 gacgggtacg tgatcgcgtc cttcacgaat ggtgagaacc gccggctcta ccagttgccg
     4201 atcgccacct tcgccaaccc gtcggggctc gatccgcgat cgggcaatgt cttcgcccag
     4261 accgaggcgt ccggcgcctt caacttgcgc gcggcggggc ggggaggtgc cggcattctc
     4321 gccccgtcgg cgctcgaagc cgccaatgtc gatctcgccg acgagttcac caagatgatc
     4381 gtcacccagc
//
LOCUS       DAOTFI010000321         4365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_23817_length_4365_cov_6.401856, whole genome shotgun sequence.
ACCESSION   DAOTFI010000321 DAOTFI010000000
VERSION     DAOTFI010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23817_length_4365_cov_6.401856"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="P5558_22145"
     CDS             complement(<1..224)
                     /locus_tag="P5558_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY27081.1"
                     /translation="MPDYVIIGGGASGAVIAARLTADPDVSVTVLEAGPSDTNRYIHM
                     PVGFAKMTGGPLTWGLVTAPQKHCENREIP"
     gene            727..1272
                     /locus_tag="P5558_22150"
     CDS             727..1272
                     /locus_tag="P5558_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27082.1"
                     /translation="MRRYALLGVLGMVLWLGGCSSPATVEGMSSLSSFGEAAAPSELH
                     HAIELGTVTGGKPTNPLLTSDVGNAEFREALAQSLRAAGILATDGSPGAYLLEATLVG
                     MELPTMGFDLSVTTRSTVDYRVSPTAGEGKSLHNVVTRPYTATFSDALSGAERVRLAN
                     EGAIRENIREFIDQLIAFYSN"
     gene            1320..1730
                     /locus_tag="P5558_22155"
     CDS             1320..1730
                     /locus_tag="P5558_22155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27083.1"
                     /translation="MGNPIRSLLVATALLGMVASTSGCSVGMALSGDENPDLTVCRVG
                     SQKSDIDRQLGPPTSVRTLPEGGQTCTYEYEIGDEPSAGRAVAHGAMDVLTFGLWEVV
                     GTPVEALQGQKYQMTVTYDAEGKAKEISTIKVAK"
     gene            1899..2543
                     /locus_tag="P5558_22160"
     CDS             1899..2543
                     /locus_tag="P5558_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY27084.1"
                     /translation="MTETATHEKPRIRDAEATQRRILAAARKEFAKSGLGGARVDTIA
                     RRAKANKRMIYHYFKSKEQLFTRVLEDAYADIRTAEQKLHLDELDPETAITTLVRFTW
                     RYYLANPEFLTLVNSENLHRARHLKGSARMHELSRPFLGMVEGILARGVAAGVFRPGI
                     DPVQLNITIAAIGYYYLTNRFTGAIVYERDLMTKEALDTRLAFNIETILRLLRA"
     gene            complement(2549..3568)
                     /locus_tag="P5558_22165"
     CDS             complement(2549..3568)
                     /locus_tag="P5558_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY27085.1"
                     /translation="MQAKTKRPFGRTGLEVTAMSFGAAPIGNFLKPIDEEVAKAMVDA
                     AWDAGIRMFDTSPLYGHGLSELRLGAALRWRRRDDYVLSTKVGRLLEPAKRETIDYAP
                     WVDAGAFKLRFDYSHDGAMRSIEDSLQRLGLERIDIALVHDCDRFNFGDELPEVFETA
                     MNGAAKALLRLRDEGVVKAVGLGVNEWQPCYDALLRHDLDAFLLAGRYTLLEQESLDP
                     FLALCAERGAGVIVGGGFNSGILATGAVPGAKYNYSPAPEDIVAKVKKIEAVCAEFDV
                     ALPAAALQFVTAHPAVPTFCAGTRNVEQLEQNVAWLEAPIPGDFWQALKARGLLREDA
                     PVPAG"
     gene            3695..>4365
                     /locus_tag="P5558_22170"
     CDS             3695..>4365
                     /locus_tag="P5558_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY27086.1"
                     /translation="MPPVLLPRHAPEDYAQIQEAARRFADEAVRPRAVELDATEAFPD
                     DLYREMAGLGLFGISVPDSLGGVGMDALAYAIVMEELSRGYASVADQCGLVELIGTLL
                     AVHGTADQQERYLPPVLGFEVKVAYCITEAGAGSDVSGIKTTATRTEGGWRLDGGKLW
                     IHNAPLADLGFVLARTDPAAGHRGMSIFIVDLNGKGVSRGPKEHKMGQRASPVGALTF
                     DGVELP"
BASE COUNT          738 a         1560 c         1363 g          704 t
ORIGIN      
        1 taaggaatct cgcggttctc gcaatgcttc tgcggcgccg tcaccagccc ccaggtcaag
       61 ggcccgcccg tcatcttggc gaagcccacc ggcatgtgga tataccggtt cgtatcgctc
      121 ggcccggcct ccagcaccgt caccgagaca tccggatcgg ccgtgagccg tgctgcgatc
      181 accgcccccg aagccccgcc gccgatgatc acgtagtcag gcatggtgaa gcggcctccc
      241 gttggtttgc gcgcaccgta cccgaacggt gccagcgttc aacggtcggc ggagaacctg
      301 tcacatagat gccagggaag gaggccacgg ccgtggcggc gaaatgggcg agctcgcgca
      361 tgctgaggga cgccagggtg cagaaggcga ggaagccggt cggcatagac ggctggccgg
      421 cccgtccatg acgaccgctt ccacgcacgg gagggcaatc gcttatcgga cgaacaacgg
      481 tttggaaggt cgctagctcg gcgctttggt ctcccgaggc tcgcctgacc ctggcgcgcg
      541 ccagacggtc caccggtcac cgattcgcag tgtttcgagc ttcagtgcag ccgttgattc
      601 agatcaacgc agttgcctcg tgaaatgtca tagtaaattt cttgaatatt tggcgatgcg
      661 gcgagggagt gtctgtccgt gtgtggcccc agtgttcgcg ccggtttctt tcgcgacccg
      721 catcggatgc gccggtatgc gctgctcggc gtcctcggca tggtgctctg gctcggcgga
      781 tgctcctcgc cggccaccgt cgaaggaatg agcagcctgt cgtcgttcgg cgaggcggca
      841 gcaccctccg agttgcacca cgccatcgag ctggggacgg ttacgggtgg caagcccacc
      901 aatccgctct tgacctccga tgtcggcaac gcggaatttc gggaagcact ggctcagtct
      961 ctgcgggcgg ccggcatcct ggcaaccgac ggatcacccg gcgcctatct gctcgaggcc
     1021 acgctcgtcg gcatggagct gccgacgatg ggcttcgacc tcagcgtcac caccaggtcg
     1081 acggtcgatt accgggtctc gccgacggct ggggagggaa aatccctcca caacgtcgtc
     1141 acgcgtccct ataccgcgac cttttccgac gccttgagcg gcgccgagcg cgtacgcctc
     1201 gcgaacgagg gcgcgatccg cgagaacatc cgcgaattca tcgaccagtt gatcgctttc
     1261 tacagcaact gatgtgccaa cctcgatccg ttcggatcga agcaaccggg agatacgtca
     1321 tgggaaatcc aattcgctct ctgcttgtcg cgactgccct gcttggtatg gttgcatcga
     1381 ccagcggctg ctccgtcggc atggcccttt ccggcgatga gaatcctgat ttgaccgtct
     1441 gtcgggtcgg cagccagaaa agcgacatcg accgccagct cgggccgccg accagtgtgc
     1501 ggactctccc cgaaggcggg cagacctgca cctacgaata cgagatcggc gacgagccgt
     1561 cggctggccg cgccgtggcg cacggggcca tggatgtgct gaccttcggc ctctgggagg
     1621 tcgtcggcac gccggtcgag gccctgcagg gacagaagta ccagatgacc gtgacctacg
     1681 acgccgaggg caaggccaag gaaatctcga ccatcaaggt cgccaagtag cgttcccggt
     1741 cccgtcgcta cccgtccggg cattcgcccc ggacggggcg atgccgccca cgcgaatccg
     1801 gccattcggc caacccgctt gcgcctcagc ccacccccga cttaactaac cggtacgtgc
     1861 attggcgact ctcttttggg tcgccggcag gagcccggat gaccgagacc gcaacacacg
     1921 aaaaaccccg catccgcgac gccgaggcga cgcagaggcg catcctcgcc gccgccagga
     1981 aggagttcgc gaagtcgggc ctcggtgggg cccgggtcga caccatcgcg cggcgggcca
     2041 aggccaacaa gcggatgatc taccactact tcaagagcaa ggagcagctc ttcacccgcg
     2101 tcctcgagga cgcctatgcc gacatccgca cggccgagca gaagctccat ctggacgagc
     2161 tcgaccccga gacggcgatc accaccctcg tccgcttcac ctggcgttac tacctggcca
     2221 accccgagtt cctcaccctc gtcaacagcg agaatctgca ccgtgcgcgc cacctcaagg
     2281 gctcggcgcg catgcacgag ctcagccggc ccttcctcgg catggtcgag ggcatcctgg
     2341 cgcgcggcgt cgccgccggc gtctttcggc ccggcatcga cccggtccag ctcaacatca
     2401 ccatcgccgc gatcggctac tactacctca ccaaccgctt caccggggcc atcgtctacg
     2461 agcgcgacct gatgacgaag gaggcgctcg acacccgcct cgccttcaac atcgagacca
     2521 tcctgcgcct gctccgcgcc tgagccgctc agccggccgg gaccggcgca tcctcccgca
     2581 ggagcccccg tgccttcagc gcctgccaga aatcgcccgg gatgggtgcc tcgagccagg
     2641 cgacgttctg ctcgagctgc tccacgttgc gcgtcccggc gcagaaggtc gggaccgccg
     2701 gatgcgccgt cacgaactgc agcgccgccg ccggcagggc cacgtcgaac tcggcgcaga
     2761 ccgcctcgat cttcttgacc tttgccacga tgtcctcggg tgccgggctg tagttgtact
     2821 tggcccccgg cacggccccc gtggcgagga tgcccgagtt gaacccgccg ccgacgatca
     2881 ccccggcgcc ccgctcggcg cagagcgcca ggaaggggtc gagcgattcc tgctccagga
     2941 gggtgtagcg gccggccagc agaaaggcgt cgagatcgtg gcgcaggagg gcatcgtagc
     3001 agggctgcca ctcgttgacc ccgagcccga ccgccttgac cacgccctcg tccctgagcc
     3061 gcagcagcgc cttcgccgcc ccgttcatcg ccgtctcgaa gacctccggc agctcgtcgc
     3121 cgaagttgaa ccggtcgcag tcgtggacga gggcgatgtc gatccgctcg agcccgagcc
     3181 gctgcagcga atcctcgatc gagcgcatcg ccccgtcatg ggaatagtcg aaacgcaact
     3241 tgaacgctcc ggcatcgacc cagggtgcat agtcgatggt ctcgcgcttc gccggctcga
     3301 gcaggcggcc gaccttggtg gagagcacgt agtcgtcgcg ccggcgccag cgcagcgccg
     3361 ccccgagccg aagctcgctg agcccatgcc cgtagagcgg cgaggtgtcg aacatgcgaa
     3421 tcccggcatc ccaggcggcg tcgaccatcg ccttcgccac ctcctcgtcg atgggcttga
     3481 ggaaattgcc gatgggtgct gccccgaagc tcatcgccgt cacctcgagc cccgtccgcc
     3541 cgaacggtct tttggtcttc gcctgcatcc cgatccctcc cgcattgcac tgtacggtta
     3601 cttagcaggg caggttgccg ccgaacagcg ccgccttgac aggaacgggt gcgaaaagta
     3661 accgtccggt tacaaaaact gccggggaac cgccatgccg cccgtcctgc tgccgcgcca
     3721 cgcgcccgag gactatgcgc agatccagga ggccgcccgc cgcttcgccg acgaggcggt
     3781 ccgcccgcgc gccgtcgagc tcgacgcgac cgaggccttt ccggacgacc tctaccgcga
     3841 gatggccggc ctcggcctct tcggcatcag cgtgcccgac agcctcggcg gcgtcggcat
     3901 ggacgcgctc gcctatgcca tcgtcatgga ggagctctcg cgcggctacg cctccgtcgc
     3961 cgaccagtgc ggcctggtcg agctgatcgg gacactcctc gccgtccacg gcacggccga
     4021 ccagcaagag cgctacctcc cgcccgtcct cggcttcgaa gtgaaggtcg cctactgcat
     4081 caccgaggcc ggcgccggct ccgacgtctc gggcatcaaa acgaccgcga cccggaccga
     4141 gggcggctgg cgcctcgatg gcggcaagct ctggatccac aacgcgcccc tggccgatct
     4201 cggcttcgtc ctcgcccgca ccgatcccgc agccggccat cgcggcatgt cgatcttcat
     4261 cgtcgacctg aatggcaagg gcgtgagccg cggcccgaag gagcacaaga tgggccagcg
     4321 cgcctcgccc gtgggtgcgc tcaccttcga cggcgtcgag ctgcc
//
LOCUS       DAOTFI010000322         4359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_23865_length_4359_cov_5.931227, whole genome shotgun sequence.
ACCESSION   DAOTFI010000322 DAOTFI010000000
VERSION     DAOTFI010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23865_length_4359_cov_5.931227"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="P5558_22175"
     CDS             <1..464
                     /locus_tag="P5558_22175"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY27087.1"
                     /translation="GLAGLDFNLGDVLMIVATVIYAAYSVALRQKPQVHWLGFVAMLV
                     GIGSLASLPFVALEALTGTFIWPRTSLAWTVIVYAAVFPSIVAQAFFIRGIEVLGSNA
                     GGLYLNLMPIMGALLSIALLGERFHLFHAAALVLVLGGIALAQRRRAPAHS"
     gene            624..1376
                     /locus_tag="P5558_22180"
     CDS             624..1376
                     /locus_tag="P5558_22180"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRY27088.1"
                     /translation="MAATLALLTSTALAGAALADEAKIQATEPATGSMATADGADATR
                     SAADQGVPGKRVPAGAEQPDEAQLEAGVNTPTDQAGGEALSQSPVNPDAQIADDPSAG
                     PGSPTYAGDQGQPGKRVTGEETTEATEATPADESREYRSYAASPDMGGAVLPAGYSAE
                     DFLDREIVNEQGEEVGEVVDLMVDGNNHITKVVADIGGFLGIGESRVAIDIEDITVEE
                     GSGDLLVMMDQSSLEALPTYEEEDGIWSPDRD"
     gene            1526..2545
                     /locus_tag="P5558_22185"
     CDS             1526..2545
                     /locus_tag="P5558_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY27089.1"
                     /translation="MQERRRLGGSEVEVTTHGFGGVPLGNLYRKCSDEDAKATLEAAW
                     AAGIRFYDTAPLYGHGMSEHRIGHFMRQHPRDEWVLSTKIGRVLKAKDPALGIDTRNF
                     VDYHPFEAVFDYTYDGVMRSFEDSLQRLATHRIDVLLVHDIDIWTHGSEAARKAKLEE
                     LMGSGYKALRELRDSGTVKAIGAGVNEVQAMLEFGRAGVFDCFLLAGRYTLLEQGALD
                     ELLPWCEEANVSLFIGGPYNSGILATGAVPGAKYNYQDAPPEIMERVGRIEAVCGRHN
                     VPLKAAALQFPLGHDRVASIIPGARSAAEINENVETFTTPIPGDLWAELKHEGLVRQD
                     APTPS"
     gene            2542..3324
                     /locus_tag="P5558_22190"
     CDS             2542..3324
                     /locus_tag="P5558_22190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY27090.1"
                     /translation="MTVLDRFRLDGKRALVTGGSRGLGRAMATALAEAGADLVIAGRD
                     AARLEEAATALGATGRKIDTVQADLGVPASCEAAIQGVLAEHGPIDILINNIGGRRDP
                     APLTEMSTARWQELIDLNLTSTFICTRLVGATMLERGRGGRIINIASINAFVAGQNIG
                     GRHYETAKAAVLQFTRAVAADWAPFGVTVNAICPGVFMTEANERWAREKPEVIEGVVA
                     RTPMGITGDPRDLGPLALYLASDASRFMTGAALVIDGGYTLW"
     gene            3365..4033
                     /locus_tag="P5558_22195"
     CDS             3365..4033
                     /locus_tag="P5558_22195"
                     /inference="COORDINATES: protein motif:HMM:NF003975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HRY27091.1"
                     /translation="MISVIPDILDFKAVALLRSELRSLRFTDGAATAGKFGQSVKKNS
                     QLEQGPQAQKIQQTILNAVLASLEFEMVARPLIVRTPLVARYEPGMAYGSHVDNAVMS
                     GRPPIRSDMSFTLFLEDPEKYDGGELVIESTYGEQEIKLPAGALVLYPTSALHRVAEV
                     TRGQRLVAVSWVQSMIRDPAQRELLHELDTARRSLFDQHGKTTEFDTITKTFHNLLRM
                     WAEV"
     gene            4092..>4359
                     /locus_tag="P5558_22200"
     CDS             4092..>4359
                     /locus_tag="P5558_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27092.1"
                     /translation="MQRWILVAVLLVVGLPVLAVAGAWLALEQGWLNGVIEARAGAAT
                     GYDIELEGAPDLAWEDGALRLTLGPLTIANAADEKLATVETAKAE"
BASE COUNT          667 a         1578 c         1471 g          643 t
ORIGIN      
        1 tcggcctcgc cggcctcgat ttcaacctgg gcgacgtctt gatgatcgtg gcgacggtga
       61 tctacgccgc ctactcggtg gccctgcgcc agaagcccca ggtgcactgg ctcggcttcg
      121 tcgccatgct ggtcggcatc ggctccctgg ccagcctgcc cttcgtcgcc ctcgaggcgc
      181 tgaccggcac cttcatctgg ccgcgcacgt cgctcgcctg gacggtcatc gtctatgccg
      241 ccgtctttcc ctcgatcgtc gcccaggcct tcttcatccg cggcatcgag gtgctcggca
      301 gcaacgcggg cggcctctat ctcaacctca tgccgatcat gggtgccctg ctctcgatcg
      361 cccttctggg cgagcgcttc cacctcttcc acgcggccgc cctggtgctg gtgctgggcg
      421 gcatcgccct ggcgcagcgg cggcgggcac ccgcgcattc ctgatcatcg ctcggctggt
      481 gcggaggagc gcttccggct cagggcggaa cctgcggttg gcggaagcgt tcagggtggc
      541 aacggcgacc caccccgatc tttttggtgc gtcgtcggat gcaatccact gaacgagaca
      601 ggagactggc tatgcgagtg cgaatggctg caaccttggc gctcttgacc tctaccgccc
      661 tggccggcgc cgccttggcg gacgaggcga agatccaggc caccgagccg gcgaccggat
      721 cgatggctac ggccgatggt gcggacgcga cccgctccgc cgccgatcag ggcgtgccgg
      781 gcaagcgtgt gccggccggt gccgagcagc ccgacgaggc gcagctcgag gcgggcgtca
      841 acacaccaac cgatcaggcc gggggcgagg cgctcagcca atctccggtg aacccggatg
      901 cgcagatcgc cgacgacccg tcggccggcc cgggctcgcc cacctatgcc ggcgatcagg
      961 gccagcccgg caagcgcgtg accggtgagg agacgaccga ggcgaccgag gcgacgcccg
     1021 cggacgagtc gcgggagtat cgctcttacg ccgcctcgcc ggacatgggt ggcgccgtcc
     1081 tgcccgccgg ctacagcgcc gaggacttcc tcgatcggga gatcgtcaac gagcagggcg
     1141 aggaggtcgg cgaggtggtc gacctgatgg tcgacggcaa caatcacatc accaaggtcg
     1201 tggcagatat cggcggcttc ctcggcatcg gcgaaagtcg tgtggccatc gacatcgagg
     1261 acatcacggt ggaggagggc agtggcgacc tcctggtgat gatggaccag tcgtcgctcg
     1321 aggccctgcc gacctacgag gaggaggacg gcatctggtc ccccgaccgc gattgagcac
     1381 gtccccgacg gccggaggac tcggagggcg cccagcgccc tcctttcttt tgtcgcgccc
     1441 cgttccatcg cctcggcgct tgacgcgggt ggtgcccgga cggttagtgg cgccgacgcg
     1501 gcacagaggg ggacgcacca tggacatgca ggaacgccgc cggctcggcg gctccgaggt
     1561 cgaggtcacg acgcacggct tcggcggggt gccgctcggc aacctctacc gcaagtgctc
     1621 cgacgaggac gcgaaggcca cgctggaggc cgcctgggcc gccggcatcc ggttctacga
     1681 cacggccccc ctctacgggc acggcatgtc cgagcaccgc atcggccatt tcatgcgcca
     1741 gcacccgcgc gacgaatggg tcctctcgac caagatcggc cgggtgctca aggcgaagga
     1801 cccggcgctc ggcatcgaca cccgcaactt cgtcgactac caccccttcg aggcggtctt
     1861 cgactacacc tatgacggcg tgatgcgctc cttcgaggac agcctgcagc gcctcgccac
     1921 gcaccgcatc gacgtgctcc tggtgcacga catcgacatc tggacgcatg gcagcgaggc
     1981 agcccgcaag gcgaagctcg aggagctcat gggctcgggc tacaaggcgc tgcgcgagct
     2041 gcgcgacagc ggcacggtca aggccatcgg cgccggcgtc aacgaggtgc aggccatgct
     2101 cgagttcggc cgcgccggcg tcttcgactg cttcctcctc gccggccgct acaccctgct
     2161 cgagcagggt gccctcgacg agctcctgcc ctggtgcgag gaggccaatg tctcgctctt
     2221 catcggcggc ccctacaatt ccggcatcct cgccaccggc gccgtgcccg gtgccaagta
     2281 caactaccag gacgcaccgc ccgagatcat ggagcgcgtc gggcggatcg aggccgtctg
     2341 cggccgccac aacgtgccgc tcaaggccgc ggcgctgcag ttcccgctcg gccacgaccg
     2401 ggttgcgagc atcatccccg gtgcccgctc ggcggcggag atcaacgaga acgtcgagac
     2461 cttcacgacg ccgatcccgg gcgacctctg ggccgagctg aagcacgagg ggctggtccg
     2521 ccaggacgcg ccgacgccgt catgacggtg ctcgatcggt ttcgcctcga cggcaagcgc
     2581 gccctcgtca ccggcggcag ccgcggcctc ggccgcgcca tggccaccgc cctggccgaa
     2641 gccggtgccg acctcgtcat cgccggccgc gacgcggcgc gcctcgagga ggcggccacc
     2701 gccctcggcg ccaccggccg caagatcgat accgtccagg ccgacctcgg cgtgccggcg
     2761 agctgcgagg cggccatcca gggcgtgctc gccgagcacg ggccgatcga catcctgatc
     2821 aacaacatcg gcggccgccg cgacccggcg ccgctcaccg agatgtcgac cgcccgctgg
     2881 caggagctga tcgacctgaa cctgaccagc accttcatct gcacgcgcct cgtcggggcg
     2941 acgatgctcg agcgcggccg gggcgggcgg atcatcaaca tcgcctcgat caacgccttc
     3001 gtcgccgggc agaacatcgg cggccgccac tacgagacgg cgaaggcggc cgtcctgcag
     3061 ttcacccggg cggtggccgc cgactgggcg cccttcggcg tcaccgtcaa cgccatctgc
     3121 ccgggggtgt tcatgaccga agccaacgag cgctgggcca gggagaagcc ggaggtgatc
     3181 gagggcgtcg tcgcccgcac gccgatgggc atcacgggcg acccccggga cctgggcccg
     3241 ctcgccctct acctcgcctc cgacgccagc cgcttcatga cgggcgccgc cctggtcatc
     3301 gacggcggct acacgctctg gtgagcggcc cgatcgcccg ccatcgccag ggaggccggc
     3361 acccatgatc tccgtcatcc ccgacattct cgatttcaag gccgtggccc tgctcaggtc
     3421 cgagctccgc tcgctgcgct tcaccgacgg tgcggcgacg gccggcaagt tcggccagtc
     3481 ggtcaagaag aacagccagc tcgagcaggg cccgcaggcc cagaagatcc agcagaccat
     3541 cctgaatgcc gtgctcgcca gcctcgagtt cgagatggtg gcccggccgc tgatcgtccg
     3601 gactccgctc gtggcgcgct acgagccggg catggcatac ggcagccatg tcgacaacgc
     3661 cgtcatgtcg ggtcgcccgc cgatccgctc cgacatgtcc ttcacgctct tcctcgagga
     3721 tcccgagaag tacgacggcg gcgagctcgt catcgagagc acctatggcg agcaggagat
     3781 caagctgccg gcgggcgcgc tcgtgctcta tcccacctcg gccctgcacc gcgtcgccga
     3841 ggtgacccgg ggccagcgcc tcgtcgcggt ctcctgggtg cagagcatga tccgcgaccc
     3901 ggcacagcgg gagctgctcc acgagctcga cacggcccgc cgcagcctct tcgaccagca
     3961 cggcaagacc accgagttcg acacgatcac caagaccttc cacaatctgc tgcggatgtg
     4021 ggccgaggtt tgaggcaacc cggccggggc tgggccgttg agcccggacc gccttttcgg
     4081 gtggagggct cgtgcagcgg tggatcctcg tcgccgttct cctggtcgtc gggctgccgg
     4141 tcctggcggt ggccggcgct tggctcgccc tcgagcaggg ctggctcaac ggcgtgatcg
     4201 aggcgcgggc gggcgccgcc acgggctatg acatcgagct ggagggtgcg ccggacctcg
     4261 cctgggagga cggcgccctg cgcctgacgc tgggcccgct caccatcgcc aatgcggcgg
     4321 acgagaagct ggcgaccgtc gagaccgcca aggctgagc
//
LOCUS       DAOTFI010000323         4327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_24108_length_4327_cov_7.186096, whole genome shotgun sequence.
ACCESSION   DAOTFI010000323 DAOTFI010000000
VERSION     DAOTFI010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24108_length_4327_cov_7.186096"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="P5558_22205"
     CDS             <1..536
                     /locus_tag="P5558_22205"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY27093.1"
                     /translation="VADASPVPVAIQNAPAYLGVGLSPEGLATLNRQHPNVTLLKGET
                     SAVEIEAMVRTTAGRYRVLNGRGGLEFTDNIRAGCVGMIPAPDCFDVQVRILEAMRDG
                     REAEAETLYREILPAITFVMQSIASLLCYGKRLVAARLGLDPATVVDRDPAMLPSDFG
                     LAITRRLAAGLGPYPSD"
     gene            638..1162
                     /locus_tag="P5558_22210"
     CDS             638..1162
                     /locus_tag="P5558_22210"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY27094.1"
                     /translation="MFRKKDDEPIPPLPSRGAMPAPPAPPAAKSPPPPARAAAAAPAE
                     AVGKRLIVGQGLSLSGDITSCDLLVVEGNVQVTLNDTRAIEIAESGRFTNGKASVEDA
                     VISGVYEGELTVSGRLLVRGTGRVDGSIRYGELEVERGGRLTGSIAMLDEPAVKPSAP
                     RPADPPKMDNPTGS"
     gene            1321..2325
                     /gene="glsA"
                     /locus_tag="P5558_22215"
     CDS             1321..2325
                     /gene="glsA"
                     /locus_tag="P5558_22215"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HRY27095.1"
                     /translation="MFVPVGVDETTAAEAVSTGHLPDAKLVGRLLNEAYERYRTLDEG
                     DVANYIPALARVPRELFGLCIVGVNGGVFPVGDAGHAFSIQSISKPFVFALVSEAIGC
                     EPAREKIGVNATGLPFNSVMAVELNGDRTMNPMVNAGAIATTSLAPGDTAGAKWQWIR
                     DGLSRFAGRTLELDEEVYRSEAETNLRNRGIAKLLEGYGRMYFDALEATDIYTRQCSL
                     LVTARDLAVMGATLADGGVNPVTKERVIGHTTTKRVLAALATAGLYERSGDWLYEIGL
                     PGKSGVSGGIVTISPGKGGLGTFAPPLDEAGNSIKGQRVTKYLSERLGLNLFASRPEV
                     "
     gene            2359..4041
                     /locus_tag="P5558_22220"
     CDS             2359..4041
                     /locus_tag="P5558_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY27096.1"
                     /translation="MSAATASVPIAQRESWVPMIAICLGQAIMSFNVAALPVSMSGMV
                     ASFGVPPTTVATGIVMYSLAVAGLVMLGAKLTQRFGATKMFRIVVAVYAVAQTLMTLS
                     PNATLMLTAQGLCGMAAAVIVPALVALIANHYQGTQQATAVGALGSARAGASALAFII
                     GGVLGTFIGWRPVFGILIVVSAIVFLLSFKLKEDKGRPDVQIDLVGVVLAAAAIILIS
                     FGFNNLNRWGLGVAGPNAPFDLLGMSPAPIMIVIGIILLQSLIVWCRRVAAAGRTPLL
                     DLRVIMSSSERAAVYTMFAVVALEAALNFSVPLYIQIVQGRTPIATAVAMMPFNLTVF
                     FAAILVVRLYGKLSPRQIGRIGFSLCTVALLWLAFVVRNDWSELPVLVGLFVFGAGQG
                     ALVTLVFNVLVTAAPKELASDVGSLRGTTQNLAAAVGTAVAGALMVGLLSSVVIRHLA
                     ENPEFPPEIQAQLNLGSINFVTNDRLQIILEGTSATPEQVAEAVRINTDARLRALKIG
                     LLVMAALAALTIIPAGRLPSYRPGQIPVNPPEPSAEEERRIAREYEAKEAAA"
     gene            4038..>4327
                     /locus_tag="P5558_22225"
     CDS             4038..>4327
                     /locus_tag="P5558_22225"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRY27097.1"
                     /translation="MKPRLLVLSLGGTITMVPSAEGGITPKLGAEELVASVPALADAA
                     EIEARSPFRLPSPSLTPEDLVAVAGEIDAAFAAGFDGAVVIQGTDTIEESAF"
BASE COUNT          651 a         1615 c         1389 g          672 t
ORIGIN      
        1 gggtcgccga tgcctcgccg gtgccggtcg ccatccagaa cgcgccggcc tatctgggcg
       61 tcggcctctc gcccgagggg ctcgccacgc tcaaccgcca gcaccccaac gtcaccctcc
      121 tcaagggcga gacctcggcg gtcgagatcg aggccatggt gcgcacgacg gcaggccgct
      181 atcgcgtcct caacggccgg ggcgggctcg agttcaccga caacatccgc gccggctgcg
      241 tcggcatgat cccggcaccc gactgcttcg acgtccaggt gcgcatcctc gaggcgatgc
      301 gggacggccg ggaggccgag gcggagacgc tctaccggga gatcctgccg gcgatcacct
      361 tcgtcatgca gtcgatcgcc tcgctgctct gctacggcaa gcgtctggtc gctgcccggc
      421 tgggcctcga ccccgccacg gtcgtcgacc gcgacccggc gatgctgccc tccgatttcg
      481 ggctcgccat cacgcggcgg ctggccgccg ggctcggacc ctaccccagc gactgatccg
      541 tctcccgggt tgccgcacgg aggttggtat ctccgcggcc agttgataca aggacagggc
      601 acagccggca ccaccaccct cggggagacc ccgtcccatg ttccgcaaga aagacgacga
      661 gccgatccca cccttgccct cgcgcggcgc catgcctgca cccccggcac cgccggccgc
      721 caagtcaccg ccgccaccgg caagggcggc ggcggccgct cccgctgagg cggtcggcaa
      781 gcgcctgatc gtcggccagg gcctcagcct gagcggcgac atcacctcgt gcgacctgct
      841 cgtcgtcgaa ggcaacgtcc aggtaacgct gaacgacacc cgcgccatcg agatcgccga
      901 gagcggccgc ttcaccaatg gcaaggcctc cgtcgaggat gccgtgatca gcggtgtcta
      961 cgagggcgag ctgaccgtca gtggccgcct tttggtgcgc ggcaccggcc gggtcgacgg
     1021 ctcgatccgc tacggcgagc tcgaggtcga gcgcggcggc cggctcaccg gctcgatcgc
     1081 catgctggac gagcctgcgg tgaagccgtc ggccccccgg ccggccgatc cgcccaagat
     1141 ggacaacccg accggctcct gacaccaccg gcattcgtca tgcccccgcg ggcatcggat
     1201 ctccggatct cgacgcccgc gggcattcgt cgcagccctc gatcatttgt aacttgacgc
     1261 ctggtccggg cacgacgacc aagtgagcgc gcgcaggcga gttcgaccag ggggtgctcc
     1321 atgttcgtgc cggtcggtgt cgacgagacc actgcggccg aagccgtctc cacgggacat
     1381 ctgccagacg cgaagctggt cggccgtctc ctgaacgagg cctacgagcg ctaccgcacg
     1441 ctcgacgagg gcgacgtcgc caattacatc ccggcgctcg cccgggtccc gcgcgagctc
     1501 ttcgggctct gcatcgtcgg cgtgaacggc ggcgttttcc ccgtcggcga tgccggccac
     1561 gccttctcca tccagagcat ctccaagccc ttcgtcttcg cgctggtcag cgaggcgatc
     1621 ggctgcgagc cggcgcgcga gaagatcggc gtcaacgcca ccggcctgcc gttcaactcg
     1681 gtcatggcgg tcgagctcaa cggcgaccgc accatgaacc cgatggtcaa tgccggtgcc
     1741 atcgccacca ccagcctggc gcccggcgac accgcgggcg ccaaatggca gtggatcagg
     1801 gacgggctct cccgcttcgc cggccgcacc ctcgagctcg acgaggaggt ctaccgctcc
     1861 gaggcggaga ccaacctgag gaaccgtggc atcgccaagc tgctcgaggg ctacgggcgc
     1921 atgtatttcg acgcgctcga ggcgaccgac atctacacca ggcagtgctc gctcctcgtc
     1981 acggccagag atctggcggt catgggggcg acactcgccg atggcggcgt caacccggtt
     2041 accaaggaac gggtgatcgg ccacaccacc accaagcgcg tgctcgccgc cctcgccacg
     2101 gccgggctct acgagcgctc cggcgactgg ctctacgaga tcggcctgcc cggcaagagc
     2161 ggtgtcagcg gcggcatcgt cacgatctcc ccgggcaagg gcggcctcgg caccttcgcg
     2221 ccacccctcg acgaggccgg caacagcatc aagggccagc gggtcaccaa gtatctatcg
     2281 gagcgcctcg ggctgaacct gttcgcctcg cgacccgagg tctgagcgtc acaaaataat
     2341 cagtcaggga aaccatccat gagtgccgcg actgcaagcg tgccaatcgc gcaacgcgaa
     2401 tcctgggtcc cgatgatcgc catctgcctc ggccaggcga tcatgtcctt caacgtcgcg
     2461 gccctcccgg tctcgatgag cggcatggtg gccagcttcg gcgtgccacc gaccaccgtc
     2521 gccaccggca tcgtcatgta ctccctcgcc gtcgccggcc tcgtcatgct gggcgccaag
     2581 ctgacccagc gcttcggcgc gacgaagatg ttccgcatcg tcgtcgccgt gtacgccgtg
     2641 gcgcagacgc tgatgacgct cagccccaac gccacgctga tgctgaccgc ccagggcctc
     2701 tgcggcatgg cggcggcggt gatcgtgccg gcgctggtgg cgctgatcgc caaccactac
     2761 cagggcacgc agcaggcgac ggccgtcggc gccctgggct cggcccgcgc cggggcgagc
     2821 gcgctcgcct tcatcatcgg cggcgtgctc ggcaccttca tcggctggcg gccggtgttc
     2881 ggcatcctta tcgtcgtctc ggcgatcgtc ttcctgctca gcttcaagct caaggaggac
     2941 aagggcaggc cggacgtgca gatcgacctc gtcggcgtcg tcctcgccgc cgccgccatc
     3001 atcctcatca gcttcggctt caacaacctc aaccgctggg gcctcggcgt cgccggcccg
     3061 aacgcgccct tcgatctgct cggcatgtcg ccggcgccga tcatgatcgt catcggcatc
     3121 atcctgctgc agagcctgat cgtctggtgc cgccgcgtcg ccgccgccgg caggaccccg
     3181 ctgctcgatc tgcgggtcat catgtcgtcc tcggagcggg ccgccgtcta caccatgttc
     3241 gccgtggtcg cgctcgaggc ggcgctcaac ttctcggtgc cgctctacat ccagatcgtc
     3301 caggggcgga ccccgatcgc gaccgcggtc gcgatgatgc cgttcaacct gacggtcttc
     3361 ttcgccgcca ttctcgtcgt ccgcctctac ggcaagctgt cgccgcggca gatcggccgg
     3421 atcggcttct cgctctgcac ggtggcgctg ctgtggctcg ccttcgtcgt ccgcaacgac
     3481 tggagcgagc tgccggttct ggtcggcctc ttcgtcttcg gcgccggcca gggtgccctc
     3541 gtcaccctcg tcttcaacgt cctcgtcacc gccgccccca aggagctggc gagcgacgtc
     3601 ggctcgctcc gtggcacgac ccagaacctc gccgccgccg tcggcaccgc cgtcgccggt
     3661 gccctgatgg tcggtctcct gagcagcgtc gtcatccggc atctggcgga aaaccccgag
     3721 ttcccgcccg agatccaggc gcagctcaac ctcggcagca tcaacttcgt caccaacgac
     3781 cggctccaga tcatcctaga agggacctcg gcgacacccg agcaggtggc ggaggcggtg
     3841 cgcatcaata ccgacgccag gctgagagca ctcaagatcg ggctgctcgt catggcagcg
     3901 ctcgccgcgc tcacgatcat cccggcggga cgcctgccga gctaccggcc cggccagatc
     3961 ccggtgaacc cgcccgagcc ctcggccgag gaggagcggc ggatcgcccg cgagtacgag
     4021 gcgaaggagg cggcagcttg aagccgcgcc tgctggtcct ctcgctcggc ggcaccatca
     4081 ccatggtgcc gagcgccgag ggcggcatca cgccgaagct gggtgccgag gagctggtcg
     4141 cctcggtgcc ggccctggcc gatgcggccg agatcgaggc gcgctcgccc ttccgcctgc
     4201 cgagcccgtc cctgacgccc gaggatctcg tcgccgtggc cggcgagatc gacgcggcct
     4261 tcgccgccgg cttcgacggt gccgtggtca tccagggcac cgacacgatc gaggaatcgg
     4321 ccttcat
//
LOCUS       DAOTFI010000324         4322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_24149_length_4322_cov_7.151160, whole genome shotgun sequence.
ACCESSION   DAOTFI010000324 DAOTFI010000000
VERSION     DAOTFI010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24149_length_4322_cov_7.151160"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="P5558_22230"
     CDS             complement(<1..507)
                     /locus_tag="P5558_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRY27098.1"
                     /translation="MLIDGKPYRTIWVAEDGVTVRVLDQAVLPHEVKVLDLADLESAA
                     VAITTMRVRGAPLIGATAAYGVALAMRADPTDAGLEAAVARLTKTRPTAVNLRWALER
                     MQKALANQPRERRVALAMEEAARICDEDVAICEAIGAHGLKLIEAVAAQKPGETVNIL
                     THCNAGWLA"
     gene            633..1487
                     /locus_tag="P5558_22235"
     CDS             633..1487
                     /locus_tag="P5558_22235"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRY27099.1"
                     /translation="MGPVLRRGMQGVEVEGLQQTLAGRGFRPGAIDGRFGPGTEAALI
                     AFQRAHGLLPDGIVGPVTRAKLEGDGAVLPTDVTGRIDLELVSAMFPYTRLDSIARHL
                     PVVLVALRQLALVDKPMVLMALATIRAETETFEPVDELPSRFNTSPGGHAFDLYDQRA
                     DLGNRGRPDGDRYKGRGFVQLTGRANYRRIGQMIGLADLLERNPERAALPGEAARVLA
                     AFLASRRRPIKEALLEGDLGRARRLVNGGRHGLDRFSEAYRIGDALVQDEVWRCPAPA
                     ASGAYAAA"
     gene            complement(1472..2764)
                     /gene="mtnK"
                     /locus_tag="P5558_22240"
     CDS             complement(1472..2764)
                     /gene="mtnK"
                     /locus_tag="P5558_22240"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046522 - S-methyl-5-thioribose kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HRY27100.1"
                     /translation="MPLATPAGYRALDEATLAAYLETVPTIAGRLGGAAADWRVAEVG
                     DGNLNLVFLVEGPNDGVCVKQALPYVRLVGDGWPLPLERAWFEHEATLIQARHAAERL
                     PAILHYDPQLYLIAMERLAPHIIMRRGMIAGTVYPAFVDHMVEYTANALYFTSDLALR
                     AGEKKRLVAQFAGNTELCKITEDLIFTDPYRIAELNRWTSPELDDVALAIRGDDGLKR
                     AVSLLKLKFLSRGEALLHGDLHTGSIMVTATDTKVIDPEFAFFGPMGFDVGKLIGNLL
                     LSYFSQAGHEQEAGERDDYRAWITATIERFWNGFERRFIELWLANRTGDAFPADLFEN
                     ADTEGSDAGRRSLGQAQHIFMRELFEDCLGYAGCSMIRRILGLAHNIDMEWIEDQGRR
                     AACERRTLGLAVDLVKQARQIPSIEAVTAKAAALQAAA"
     gene            2881..3939
                     /locus_tag="P5558_22245"
     CDS             2881..3939
                     /locus_tag="P5558_22245"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY27101.1"
                     /translation="MAGLATALARPVLWIGFMVGSAFVGSVLIFGSRFLIPLAIAVLI
                     WLLINAIGRAYGRVGIGALRLPPALCRLLAVLTMLYFGWLIIGISASNIAQVRAAAPV
                     YQANMNQLIARALGAFGIERTPTVTELVGRVDLVGLLGSLTAGLGSLVGSAGLVMLYV
                     AFLMLEQSSFQRKLGALFTDPLRTASLRQSLLEIERRIEGYLWIKTLVSLLTAVLCYA
                     VLATVGVDFASFWALLIFLFNFIPSIGSLFAVLLPTLLTLLQFGDIGIFLLVLACLSL
                     IQAVLGNLVEPRLMGRTLNLSPLVIILSLTLWGSIWGVAGMFLCVPLTALLAIVLSKF
                     EPTRPVAILLSSDGRLAD"
     gene            4011..>4322
                     /locus_tag="P5558_22250"
     CDS             4011..>4322
                     /locus_tag="P5558_22250"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY27102.1"
                     /translation="MRVQLRTCVRVALLLLVAGLPATGRAQLPPDPYAIGLEHFYAGR
                     YTEALPYFVTALDQSEERFGEGAPETATDLNNLAELYRLLGRYDEAEPLYLRALALDE
                     AK"
BASE COUNT          600 a         1561 c         1435 g          726 t
ORIGIN      
        1 ggcgagccag ccggcattgc agtgcgtgag gatgttgacc gtctcgcccg gcttctgcgc
       61 cgccaccgcc tcgatcagct tcagaccgtg cgccccgatc gcctcgcaga tcgccacgtc
      121 ctcgtcgcag atccgcgccg cctcctccat ggccagcgcc acccgccgct cgcgcggctg
      181 gttggcgagc gccttctgca tccgctccag cgcccagcgc aggttgaccg ccgtgggtcg
      241 ggtcttggtc agccgcgcga cggccgcctc caggccggca tcggtcggat cggcgcgcat
      301 ggcgagggcg accccgtagg ctgccgtcgc cccgatcagc ggcgcacccc gaacccgcat
      361 cgtggtgatc gccacggcgg cgctctcgag gtccgcgagg tcgagcacct tcacctcgtg
      421 gggcagcacg gcctggtcga gcacccgcac cgtcaccccg tcctccgcga cccagatggt
      481 ccgatagggc ttgccgtcga tcaacatgga ggtctcctca aggctttccg cgccggcttg
      541 atagagcatg gcgaggatcg gcacgagcct cgcttgccag ggtagggcga gtctgatata
      601 ggaaaaaaat catacatctt gcgaggtgct gcatgggtcc ggtccttcgg cgtgggatgc
      661 agggtgtcga ggtcgaaggc ctgcagcaga cactggcggg caggggcttc cgtccgggcg
      721 ccatcgacgg ccggttcggc ccgggcaccg aggcggcgct catcgctttc cagcgggccc
      781 acggcctcct gccggatggc attgtcggcc cggtcacccg ggcgaagctc gagggcgacg
      841 gtgccgtgct gccgaccgac gtcaccggcc ggatcgacct cgagctggtc tcggccatgt
      901 tcccctacac gcgcctcgac agcatcgccc ggcatctgcc ggtcgtgctg gtggcgctcc
      961 gccagctcgc cctcgtcgac aagcccatgg tgctcatggc gctggcgacc atccgtgccg
     1021 agaccgagac cttcgagccg gtcgacgagc tgccgtcgcg cttcaacacc tcgcccggcg
     1081 gccacgcctt cgacctctac gaccagcgcg ccgatctcgg caatcgcggc cggccggacg
     1141 gcgaccgcta caagggcagg ggcttcgtcc agctcaccgg ccgggccaac taccggcgca
     1201 tcggccagat gatcgggctc gccgacctgc tcgagcgcaa cccggagcgg gcggccctgc
     1261 cgggcgaggc ggcgcgcgtg ctcgcggcct tcctcgccag ccgacgccgg ccgatcaagg
     1321 aggcgctgct cgagggcgac ctcggccgcg cccggcggct ggtcaatggc ggccggcacg
     1381 ggctcgaccg gttcagcgag gcgtaccgga tcggcgacgc cctggtccag gacgaggtgt
     1441 ggcgctgccc ggcgcctgcc gccagcgggg cctacgccgc cgcctgaagc gctgcggcct
     1501 tcgccgtcac cgcctcgatc gacgggatct ggcgggcctg cttgacgagg tcgacggcga
     1561 ggccgagcgt gcggcgctcg caggcggccc gccggccctg gtcctcgatc cactccatgt
     1621 cgatattgtg ggcgagcccg agaatgcggc ggatcatcga gcagccggca tagccgaggc
     1681 agtcctcgaa gagctcgcgc atgaagatgt gctgcgcctg gccgagcgac cggcggcccg
     1741 cgtccgagcc ctccgtgtcg gcgttctcga agagatccgc cggaaaggcg tcgccggtgc
     1801 ggttggcgag ccagagctcg atgaagcggc gctcgaagcc gttccagaag cgctcgatcg
     1861 tcgccgtgat ccaggcccgg tagtcgtccc gctcgcccgc ctcctgctcg tgcccggcct
     1921 ggctgaaata gctgagcagg agattgccga tcagcttgcc gacgtcgaag cccatcggcc
     1981 cgaagaaggc gaactcggga tcgatgacct tcgtgtcggt agcggtcacc atgatcgagc
     2041 cggtatgcag gtcgccgtgc aggagcgcct cgccgcggct gaggaacttg agcttgagca
     2101 gcgagaccgc ccgcttgagc ccgtcgtcgc cgcggatggc cagggccacg tcgtcgagct
     2161 ccgggctggt ccagcgattg agctcggcga tccggtaggg atcggtgaag atcagatctt
     2221 ccgtgatctt gcagagctcg gtgttgccgg cgaactgggc caccagccgc ttcttctctc
     2281 ccgcccggag cgccaggtcg gaggtgaagt agagcgcgtt cgccgtgtac tcgaccatat
     2341 ggtcgacgaa cgccggatag accgtgccgg cgatcatgcc gcgccgcatg atgatgtgcg
     2401 gcgccaggcg ctccatcgcg atgagataga gctgcgggtc gtagtgcagg atcgccggca
     2461 gccgctcggc cgcatgccgc gcctggatga gcgtcgcctc gtgctcgaac caggcccgct
     2521 ccagcggcag gggccagccg tcgcccacca ggcgcacata gggcagggcc tgcttgacgc
     2581 agacgccgtc attcgggcct tcgacgagga agacgagatt gaggttgccg tcgcccacct
     2641 cggcgacccg ccagtcggcg gcggccccgc cgagccggcc ggcgatggtc ggcacggttt
     2701 ccaggtaggc ggcgagcgtg gcctcgtcga gtgctcgata acctgctggc gtcgcaagag
     2761 gcattcggca tcacctttcg tgcggccgac acaaggcgcg ccgccgcatc caatgtttcg
     2821 agcgggcgtg ctatggcaga tcacgccagc gacggcaaac cggtcgaacc aggggatatc
     2881 atggcagggc tcgcgacggc actggctcgc ccggtcttgt ggatcggctt catggtcggc
     2941 tcggcgttcg tcgggtcggt cctgatcttc ggcagccgct tcctcatccc gctcgccatc
     3001 gccgtgctca tctggctgct gatcaacgcg atcggccgtg cctatggccg tgtcggcatc
     3061 ggcgcgcttc gcctgccgcc cgccctctgc cggctcctcg ccgtcctcac catgctctat
     3121 ttcggctggc tgatcatcgg catcagcgcc agcaacatcg cccaggtgcg cgccgcggcc
     3181 cccgtctacc aggccaacat gaaccagctc atcgcccggg ctctcggcgc tttcgggatc
     3241 gagcggacac ccaccgtcac cgagttggtc ggccgcgttg atctcgtcgg gctgctcggc
     3301 tcgctcaccg ccggcctcgg ctcgctggtc ggcagcgccg gcctggtcat gctctacgtg
     3361 gccttcctga tgctcgagca gtcgagcttc cagcgcaagc tcggcgccct cttcaccgac
     3421 cctctgcgca ccgcgagcct gcggcagtcg ctcctcgaga tcgagcggcg gatcgagggc
     3481 tatctctgga tcaagacgct ggtcagcctg ctcaccgccg tcctctgcta tgccgtgctg
     3541 gcgacggtcg gggtcgactt cgccagcttc tgggcgctcc tgatcttcct cttcaacttc
     3601 attcccagca tcggctcgct cttcgccgtg ctcctgccga ccctcctgac gctcctgcag
     3661 ttcggcgata tcggcatctt cctcctcgtg ctcgcgtgcc tgagcctgat ccaggccgtg
     3721 ctcggcaacc tggtcgagcc gcggctcatg gggcgcaccc tcaacctgag cccgctggtc
     3781 atcatccttt cgctcaccct ctggggcagc atctggggcg tcgccggcat gttcctctgc
     3841 gtgccgctga cggcgctcct ggccatcgtc ctgagcaagt tcgagccgac ccgcccggtc
     3901 gcgatcctgc tctccagcga cggccggctc gccgactgac gcagccatgg acgacgtagc
     3961 cgcgagcctc taagagcgag cccgtgtatg gcctcatgga acatcgcacg atgcgggtgc
     4021 agcttcggac ctgtgtccgc gtggccctgc tgctgctggt cgccggcctg cccgccaccg
     4081 gccgggcgca actgccgccc gatccctatg ccatcgggct cgagcatttc tatgccgggc
     4141 gctacaccga ggccctgccg tatttcgtca ccgccctcga tcagtccgag gagcgcttcg
     4201 gcgagggcgc gcccgagacg gcgaccgacc tgaacaacct ggccgagctc taccggctgc
     4261 tcggccgcta cgacgaggcc gagcccctct atctgcgcgc cctggcgctg gacgaggcga
     4321 ag
//
LOCUS       DAOTFI010000325         4238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_24756_length_4238_cov_7.596462, whole genome shotgun sequence.
ACCESSION   DAOTFI010000325 DAOTFI010000000
VERSION     DAOTFI010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24756_length_4238_cov_7.596462"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="P5558_22255"
     CDS             complement(<1..131)
                     /locus_tag="P5558_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRY27103.1"
                     /translation="MARSIPIERYRNIGIMAHIDAGKTTTTERILYYTGKSHKIGEV"
     gene            complement(162..629)
                     /gene="rpsG"
                     /locus_tag="P5558_22260"
     CDS             complement(162..629)
                     /gene="rpsG"
                     /locus_tag="P5558_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRY27104.1"
                     /translation="MRRRAAEKREVLPDPKFNDLVVTKFINCLMEAGKKSVAEAIFYS
                     ALDKMQSRSGKDALEMFHEAVDNVKPTLEVKSRRVGGATYQVPIEVRPERRQALALRW
                     LISTAQKRAEKTMTDRLANELMDAAQGRGAAFKKREDTLRMAEANRAFSHYRW"
     gene            complement(654..1025)
                     /gene="rpsL"
                     /locus_tag="P5558_22265"
     CDS             complement(654..1025)
                     /gene="rpsL"
                     /locus_tag="P5558_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRY27105.1"
                     /translation="MPTTNQLVNQGRKVPAKRNKVPAMQACPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTSYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGTL
                     DTQGVNGRKQGRSKYGVKRPK"
     gene            complement(1341..>4238)
                     /gene="rpoC"
                     /locus_tag="P5558_22270"
     CDS             complement(1341..>4238)
                     /gene="rpoC"
                     /locus_tag="P5558_22270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRY27106.1"
                     /translation="HRLGIQAFEPMLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLS
                     LEAQLEARVLMMSTNNILSPANGKPIIVPTQDIVLGLYYLSLVIENEPGEGMAFSDVN
                     EVQHALAAGTVTLHSKVKCRIDTVGAEGQVENRLVDSTPGRMLLAEILPAHREVNFEA
                     LLNRVLTKKEISNVIDVVYRHCGQKETVIFADRLMQLGFTYAAKAGISFGKDDMIIPA
                     GKQMQVSQTQELVKQYEQQYADGLITKGEKYNKVVDAWQGCSDKVADEMMEAIQSVQH
                     SKGETGRFKQPNSVWMMAHSGARGSPAQMKQLAGMRGLMAKPSGEIIETPIISNFKEG
                     LSVLEYFNSTHGARKGLADTALKTANSGYLTRRLVDVAQDCVIVEEDCATERFITLNA
                     APDEELGERVLGRTVAEDVIAPQTGDVVIPAQTLVDEVLARRVDALRIDEIKVRSVLT
                     CESSGGVCGACYGRDLARGTPVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAAQGQA
                     EQSAVEAPVAGEVKVINPRLVTDSAGRLVALGRNSEIVLYDEVGREKLRHKLPNGARV
                     RVAPDSRVEKDQIIAEWDPHTLPVVAEAEGIVAFQDMVEGISFREVQDETTGKASKEV
                     VDWRQNARSGNLRPSILLQDEEGKPITLPSGNEARHYLPVAAIFTIENGAKVRAGDVL
                     ARLPKEASRTRDITGGLPRVAELFEARKPKDPAIIAEIDGKVEFGKDYKNKRRLKLVP
                     ADEDLEPVEYLIPKGRHVAVQEGDLVKPGDPLVDGTPVPHDILRVLGVEALAQFLVHE
                     IQEVYRLQGVTINDKHIEAIVRQMLQKIEVIDPGDTTFLVGEQVDRIEFDRVNHRAVL
                     EDRRPATAFPVLQGITKASLQTQSFVSAASFQETTRVLTDAAFSGKIDRLQGLKENVI
                     VGRLIPAGTGGVLNRIKKLKAGDERFAAPIEVGPDEDPMRLPAFSELPSAE"
BASE COUNT          598 a         1457 c         1393 g          790 t
ORIGIN      
        1 tgcacctcgc cgatcttgtg cgacttgccg gtgtaataga ggatgcgctc cgtggtcgtc
       61 gtcttgcccg cgtcgatgtg ggccatgatg ccgatgttgc ggtagcgctc gatgggaatg
      121 ctgcgtgcca tgaatttcgt tccgagccgg gttcgccggc cttaccagcg gtaatgcgag
      181 aaagcgcgat tggcctcggc catgcgcagc gtgtcctcgc gtttcttgaa ggcggcgccg
      241 cgaccctgcg cggcatccat gagctcgttg gcgagccggt cggtcatggt cttctcggcc
      301 cgcttctggg cggtcgagat cagccagcgc agggccagcg cctgccgtcg ctccggccgc
      361 acctcgatcg gcacctggta ggtggcgcca ccgacgcggc gtgacttgac ctcgagggtc
      421 ggcttcacgt tgtcgaccgc ctcgtggaac atctccagcg catccttgcc cgaccgcgac
      481 tgcatcttgt cgagggcgct gtagaagatc gcctcggcca ccgacttctt gccggcttcc
      541 atcaggcaat tgatgaattt cgtgaccacc aaatcgttga acttggggtc tgggagaact
      601 tcccgcttct cggccgcacg acgccgcatc gttcgtcttc ctcgtcttca ggcttacttg
      661 gggcgcttca cgccgtactt cgaacggccc tgcttgcggc cgttcacacc ctgcgtatcg
      721 agggtgccgc ggatgatgtg gtagcgcacg cccggcaggt ccttgacgcg gccaccgcgg
      781 atgagcacca ccgagtgctc ctgcaggttg tgaccttcgc cggggatgta gctggtgacc
      841 tcgaagccgt tggtcagccg gacacgcgca accttgcgca aggccgagtt cggcttcttc
      901 ggcgtcgtcg tgtagacgcg cgtgcacacc ccgcgcttct gcgggcaggc ctgcatggcc
      961 ggcaccttgt tccgcttcgc cgggaccttc cgtccctggt tcaccaactg gttcgtcgtc
     1021 ggcatatccg agctttccct agtcgagcgg acggcgacgc gcgaccacgc tgccgctacc
     1081 gtcgccacat gaacaagaga agcggccgaa tgccagaagg cctcggccac tgccgcttgc
     1141 ttcgatgtcc gtcgccgtgt ttccggtgca gcgtctgatc cgcgagggtc accgcctgca
     1201 cccgagatga gcgtgagggg ctagttaggc ccgtgcagcg gcggcgtcaa ggacaaaccc
     1261 gggcagtcgc cctgcggtcc cgacgtcgca ggcccgcgcc ccaaggagaa ggggccgccc
     1321 tcgcgggcgg cccctctcgc ctactcggcc gatggcagct ccgagaacgc cggcaggcgc
     1381 atcgggtcct cgtccggccc cacctcgatc ggggcggcga accgctcgtc gcccgccttc
     1441 agcttcttga ttcggttcag gacaccgccg gtgccggccg ggatcaggcg gccgacgatg
     1501 acgttctcct tgagcccctg caggcggtcg atcttgccgg agaacgcggc atcggtgagc
     1561 acccgcgtgg tctcctggaa ggacgccgcc gagacgaagc tctgcgtctg caggctcgcc
     1621 ttggtgatgc cctggagcac cgggaaggcc gtggccgggc gccggtcctc gagcaccgcg
     1681 cgatggttga cccgatcgaa ctcgatgcgg tcgacctgct cgccgaccag gaaggtcgtg
     1741 tcgcccgggt cgatcacctc gatcttctgc agcatctggc ggacgatcgc ctcgatgtgc
     1801 ttgtcgttga tggtcacacc ctgcagccgg tagacctcct ggatctcgtg caccaggaac
     1861 tgggccaggg cctcgacgcc gagcaccctg agaatgtcgt gcggcaccgg cgtgccgtcg
     1921 accaggggat cgccgggctt gaccagatcg ccctcctgca ccgccacgtg gcggcccttg
     1981 gggatcaggt actcgacggg ctcgagatct tcgtccgccg gcaccagctt gagccggcgc
     2041 ttgttcttgt agtccttgcc gaactcgacc ttgccgtcga tctcggcgat gatcgccggg
     2101 tccttcggct ttctcgcctc gaacagctcg gccacgcgcg gcagaccgcc ggtgatgtcg
     2161 cgcgtccggg acgcctcctt cggcagccgg gcgagcacgt cgccggccct gaccttggcc
     2221 ccgttctcga tggtgaagat ggccgccacc ggcagatagt gccgcgcctc gttgcccgac
     2281 ggcagggtga tcggcttgcc ctcctcgtcc tgcagcagga tcgacggccg gagattgccc
     2341 gaccgggcgt tctgccgcca gtccaccacc tccttcgagg ccttgccggt ggtctcgtcc
     2401 tgcacctcgc ggaacgagat gccctcgacc atgtcctgga aggcgacaat gccctcggcc
     2461 tcggccacca ccggcagggt gtgggggtcc cactcggcga tgatctggtc cttctcgaca
     2521 cggctgtccg gcgcgacccg gacgcgggca ccattcggca gcttgtggcg cagcttctcg
     2581 cggccgacct cgtcgtagag gacgatctcc gagttgcggc cgagagccac cagccggccg
     2641 gcgctgtcgg tgacgaggcg cggattgatg accttgacct cgccggcgac cggcgcctcg
     2701 accgccgact gctcggcctg gccctgcgcc gcgccgccga tatggaaggt gcgcatggtg
     2761 agctgggtgc ccggctcgcc gatcgactgg gcggcgatga cacccaccgc ctcgccgata
     2821 ttgaccggcg tgccacgcgc caggtcacgg ccgtagcagg cgccgcagac cccgccgctg
     2881 ctctcgcagg tgagtaccga gcgcaccttg atctcgtcga tcctgagcgc gtcgacgcgc
     2941 cgcgccagca cctcgtcgac cagcgtctgc gcgggaatga ccacgtcgcc ggtctgcggg
     3001 gcgatcacgt cctcggccac cgtccggccg agcacgcgct cgccgagctc ctcgtcgggc
     3061 gccgcgttca gggtgatgaa ccgctcggtg gcgcaatcct cctcgacgat gacgcagtcc
     3121 tgcgccacgt cgaccaggcg gcgggtcaga tagcccgagt tggccgtctt gagcgccgtg
     3181 tcggccaggc ccttgcgcgc cccgtgcgtc gagttgaagt actcgagcac cgagaggcct
     3241 tccttgaagt tcgagatgat cggcgtctcg atgatctcgc ccgaaggctt ggccatgagg
     3301 ccgcgcatgc cggcgagctg cttcatctgg gccggcgagc cacgggcgcc ggaatgcgcc
     3361 atcatccaga ccgagttcgg ctgcttgaag cggccggtct cgcccttgct gtgctggacc
     3421 gactggatcg cctccatcat ctcgtcggcg accttgtccg agcagccctg ccaggcgtcg
     3481 accaccttgt tgtacttctc gcccttggtg atcaggccgt cggcgtactg ctgctcgtac
     3541 tgcttgacca gctcctgggt ctggctgacc tgcatctgct tgccggccgg gatgatcatg
     3601 tcgtccttgc cgaacgagat gcccgccttg gccgcgtagg tgaagcccag ctgcatcagc
     3661 cggtcggcga agatcaccgt ctccttctgc ccgcaatggc gatagacgac gtcgatgacg
     3721 ttggagatct ccttcttcgt cagcacccgg ttgagcaggg cctcgaagtt gacctcgcga
     3781 tgcgccggca ggatctcggc gagcagcatc cgcccgggcg tgctgtcgac gagccggttc
     3841 tcgacctggc cctcggcgcc gaccgtgtcg atccggcact tgaccttgct gtgcagggtg
     3901 acggtgcccg cggccagcgc gtgctggacc tcgttgacgt cggagaaggc catgccctca
     3961 cccggctcgt tctcgatcac cagcgacagg taatagaggc cgagcacgat gtcctgggtc
     4021 ggcacgatga tcggcttgcc gttcgccggg ctcaggatgt tgttggtcga catcatgagc
     4081 acgcgggcct cgagctgggc ctccagcgag agcggcacgt ggaccgccat ctggtcgccg
     4141 tcgaagtcgg cgttgaaggc ggtgcagacg agcggatgga gctggatcgc cttgccctcg
     4201 atcagcatcg gctcgaacgc ctggatgccg aggcggtg
//
LOCUS       DAOTFI010000326         4225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_24856_length_4225_cov_6.007194, whole genome shotgun sequence.
ACCESSION   DAOTFI010000326 DAOTFI010000000
VERSION     DAOTFI010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24856_length_4225_cov_6.007194"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="P5558_22275"
     CDS             complement(<1..619)
                     /locus_tag="P5558_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY27107.1"
                     /translation="MADPLIEARHLVRVFGGGRTWWGARKPELRAVDGVSLAVQPGTT
                     LGIVGESGCGKSTLARMLVGLIAPTEGTIRLEGRPVSKLAGAEKRRFHQTVQLVFQDP
                     IASLNPRKTVRQILEGPLEALTDLDRAGRRERVARLMDEVGLRPEFALRYPHEFSGGQ
                     AQRIGIARALAVEPRLIVLDEPVSALDVSVQAQVLGLLKELQALRG"
     gene            complement(612..2513)
                     /locus_tag="P5558_22280"
     CDS             complement(612..2513)
                     /locus_tag="P5558_22280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HRY27108.1"
                     /translation="MAASSLPRRTSAWARLSRNGLAVVGLAIVVAIVGIALLAPVLPL
                     ADPDITAPANRLLPPFSHGAWLGTDQLGRDMLSRLVWGARVSIAVGVAATLVAAFVGS
                     TIGLVAAYFGRLADNVLMRGVDMLMAFPYLLLALAIVAALGPGLLNALLAIAVVNIPF
                     FARSVRGATLGLVQREFIEAARLSGYSNARIILSELLPNVLPVIVITMSTTVGWMILE
                     TAGLSFLGLGAQPPQADLGSMLGDGRNLVITAPHVSIVPGVLILVLVIGINLLGDGLR
                     DVLDPRLKSGALSRPVARTAVAEGRTSGTGDKGALLEVRNLRTEFHIGQRVLKAVGGI
                     DFVLQPGESLGIVGESGSGKSVTALSILGLVPTPPGIVAGGEIRFAGRDLLRLPMRDL
                     QDIRGNRIAYIFQDPLTTLNPLMRVGHQVTETLIRHQGLGGSAARRQAEELLDAVGIP
                     QPRTKFDAFPHELSGGQRQRVVIAMALANRPDLIIADEPTTALDVTTQARVLALLNDL
                     RRQSGAALVFITHDLGVVSELCERVLVMYAGKVVESGPVGEVFAAPRHPYTRALLACV
                     PVLGEPERALDAIPGLPPPVDDLPEGCAFAPRCPLAMAECRRGEILLARTGEGRRSRC
                     IRWMELGDG"
     gene            complement(2513..3463)
                     /locus_tag="P5558_22285"
     CDS             complement(2513..3463)
                     /locus_tag="P5558_22285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27109.1"
                     /translation="MLGYALRRLALTVPVLLGISVIVFLVISLIPGDPATAILGSFAT
                     PENVERINRELGLDQPLPVQYLTWLQGVLEGDLGRSYSLNRPVLDEVLERFGPTLILA
                     AAALFLCTVLGLAAGVVSAVRQYGWADKLITLTVLVGISTPSFFLGIMLILWFSVGLG
                     WFPTGGMYDLFGDGSPSELLWHLTLPATALAVVATGVVARLTRSGMLEVLRQDYVRTA
                     RAKGLREGRVILRHAFVNALVGIVPIIGIQAGFVLGGAVYIETVFQWPGIGRMLVTAI
                     STRDILLVQGGVLVVAASFVLINLATDLIQVALDPRLRRG"
     gene            complement(3468..>4225)
                     /locus_tag="P5558_22290"
     CDS             complement(3468..>4225)
                     /locus_tag="P5558_22290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY27110.1"
                     /translation="GPHLWFLILNTREAPFDDVRMRQAVNYAIDKESLVTNVLQGTAT
                     VAAGPTPEAFAWAYNPDLEPYPYDPEKAKALIAEAGYDDGVETVFYVTEGGSGMLDPV
                     PMGTAIQADLAAVGIDARIETYEWNTFLGQVNPGLEGKAGMAEMAWMTNDPDTLPFLA
                     LRTEAMPENGGFNSGYYSNPMVDELLEAARRATDQEERARLYKEMQAIVHEDAPWAFI
                     ANWKQNAVTTDKVESFSLEPSFLLILKDVAKAG"
BASE COUNT          675 a         1330 c         1666 g          554 t
ORIGIN      
        1 ggccgcgaag cgcctgcagc tccttcagga ggcccagcac ctgcgcctgg accgagacgt
       61 cgagggcgga gaccggctcg tcgagcacga tcagccgggg ctcgacggca agggcacggg
      121 cgatgccgat gcgctgcgcc tggccaccgg agaactcgtg cgggtagcga agggcgaact
      181 ctggcctgag gccgacctcg tccatcagcc gggccacccg ctcgcggcgg ccggcacggt
      241 cgaggtcggt cagcgcctcg agcgggccct cgaggatctg gcgcacggtc ttcctcgggt
      301 tgagcgaggc gatcgggtcc tggaagacga gctgcacggt ctggtggaag cgccgcttct
      361 cggctccagc gagcttgctc acagggcgcc cctccagccg gatcgtgccc tccgtcgggg
      421 cgatgaggcc gaccagcatg cgggcgaggg tggacttgcc gcagccggat tcaccgacga
      481 tgccgagggt ggtgccgggc tggacggcga ggctgacgcc gtcgacggcg cgcagctcgg
      541 gctttctcgc cccccaccag gtccgcccgc cgccgaagac gcgaaccagg tgccgggctt
      601 cgataagggg gtcagccatc acccagctcc atccagcgga tgcagcggct gcgccggcct
      661 tcgccggtcc gggccagcag gatctcgccc cggcggcatt cggccatggc gagcgggcag
      721 cgcggggcga aggcgcagcc ctcgggcagg tcgtcgaccg gcggcggcag gccggggatg
      781 gcgtcgaggg cgcgctcggg ctcgcccagc accggcacgc aggcgaggag ggcgcgcgtg
      841 tagggatggc ggggggcggc gaagacctcg cccaccgggc cgctctcgac caccttgccg
      901 gcatacatga cgagcacccg ctcgcagagc tcggagacga cgccgagatc gtgggtgatg
      961 aagacgaggg cggcgccgct ctgccggcgg agatcgttca gcagggccag gacgcgggcc
     1021 tgggtggtga cgtcgagggc ggtggtcggc tcgtcggcga tgatcaggtc ggggcggttg
     1081 gcgagcgcca tggcgatgac cacgcgctgg cgctggccgc ccgatagctc gtgcgggaag
     1141 gcgtcgaact tcgtgcgcgg ctgggggatg ccgacggcgt cgagaagctc ctccgcctga
     1201 cgccgggcgg cgctgccgcc caggccctgg tggcggatca gcgtctccgt gacctggtgg
     1261 ccgacccgca tgagcgggtt cagcgtggtc agcgggtcct ggaagatata ggcgatccgg
     1321 ttgccgcgga tgtcctgcag gtcgcgcatg ggcaggcgca ggagatcgcg gccggcgaag
     1381 cggatctcgc cgccggcgac gatgccgggc ggggtcggca cgaggccgag gatcgagagt
     1441 gctgtcaccg acttgccgga gccgctctcg ccgacgatgc cgaggctttc gcccggctgc
     1501 agcacgaaat cgatgccgcc caccgccttc agcacgcgct ggccgatgtg gaactcggtc
     1561 cggaggttcc tgacctcgag cagggcaccc ttgtcgcccg tgccggaggt gcggccctcc
     1621 gcgacggcgg tgcgggccac ggggcgcgag agggcgccgg acttgaggcg cgggtcgagc
     1681 acgtcgcgca gcccgtcgcc gagcaggttg atgccgatga cgaggacgag gatcagcaca
     1741 cccgggacga tcgagacatg cggggcggtg atgacgaggt tcctgccgtc gcccagcatc
     1801 gagccgagat cggcctgggg cggctgggcg ccgaggccga ggaaggagag cccggcggtc
     1861 tcgaggatca tccagccgac ggtggtcgac atggtgatga cgatgaccgg cagcacgttg
     1921 ggcaggagct cggagaggat gatccgcgcg ttcgagtagc cggagagccg ggccgcctcg
     1981 atgaactcgc gctgcacgag gccgagcgtg gcgccccgga ccgagcgggc gaagaagggg
     2041 atgttgacga ccgcgatggc gaggagcgcg ttcaggaggc ccggccccaa tgcggcgacg
     2101 atggccagcg ccagcaggag gtagggaaag gccatcagca tgtcgacgcc gcgcatcagc
     2161 acgttgtcgg caagccgccc gaaataggcg gcgacgaggc cgatcgtgct gccgacgaag
     2221 gcggcgacga gcgtggcggc gacgccgacc gcgatgctga cccgggcccc ccagacgagc
     2281 cgggacagca tgtcgcggcc gagctggtcg gtgccgagcc aggcgccatg ggagaagggc
     2341 ggcaggaggc ggttcgccgg ggcggtgatg tcgggatcgg cgagcggcag gacgggggcg
     2401 aggagcgcga tgcccacgat agcgacgacg atggcgaggc cgacgacggc gagcccgttg
     2461 cggctgagcc tggcccaggc ggaggtgcgg cggggaagtg aactcgccgc catcagccgc
     2521 gcctcaggcg ggggtcgagg gcgacctgga tcaggtccgt ggcgaggttg atgaggacga
     2581 aggacgcggc caccacgagg acgccgccct gcaccaggag gatgtcgcgg gtcgagatgg
     2641 cggtgaccag catccggccg atgccgggcc actggaagac ggtctcgata tagacggcac
     2701 cgccgaggac gaagcccgcc tggatgccga tgatcgggac gatgccgacg agcgcgttca
     2761 cgaaggcgtg gcggaggatg acgcggcctt cgcgcaggcc cttggcccgg gcggtgcgca
     2821 cgtagtcctg gcgcagcacc tcgagcatgc cggaccgcgt cagccgggcg acgacgccgg
     2881 tcgcgaccac ggcgagggcg gtggcgggca gggtcaggtg ccagagcagc tcgctcgggc
     2941 tgccgtcgcc gaagaggtcg tacatgccgc ccgtcgggaa ccagccgagg ccgaccgaga
     3001 accagaggat cagcatgatg ccgaggaaga aggagggggt ggagatgccg acgagcacgg
     3061 tgagcgtgat gagcttgtcg gcccagccat actggcggac cgccgagacg acgccggcgg
     3121 cgaggccgag gacggtgcag aggaagaggg cggcggccgc caggatcagc gtcgggccga
     3181 agcgctccag cacctcgtcg aggaccggcc ggttgagcga gtaggagcgg ccgagatcgc
     3241 cctcgaggac accctggagc caggtcaggt actggacagg caggggctgg tcgaggccga
     3301 gctccctgtt gatgcgctcg acgttctccg gcgtggcgaa ggagcccagg atggccgtcg
     3361 ccgggtcgcc cgggatcagg gagatcacca gaaagacgat caccgagatc ccgagcagga
     3421 ccgggacggt cagggccagc cgacgaaggg cgtagccgag caaggggtca gccggccttg
     3481 gcgacgtcct tgaggatcag gaggaagctg ggctcgaggc tgaagctctc gaccttgtcg
     3541 gtggtcacgg cgttctgctt ccagttggcg atgaaggccc agggcgcgtc ctcgtggacg
     3601 atcgcctgca tctccttgta gagccgcgcc cgctcctcct ggtcggtggc gcgccgcgcc
     3661 gcctcgagca actcgtcgac catcgggttc gagtagtagc ccgagttgaa gccgccattc
     3721 tccggcatgg cctcggtcct gagcgccagg aagggcaggg tgtcggggtc gttggtcatc
     3781 caggccatct cggccatgcc ggccttgccc tcgaggccgg ggttcacctg gccgaggaag
     3841 gtgttccact cgtaggtctc gatcctggcg tcgatgccga cggcggcgag gtcggcctgg
     3901 atggccgtgc ccatcggcac cgggtcgagc atgccggagc cgccctcggt gacgtagaag
     3961 acggtctcga caccatcatc gtagccggcc tcggcgatca gcgccttggc cttctcgggg
     4021 tcatagggat agggctcgag gtcggggttg taggcccagg cgaaggcctc gggcgtcggc
     4081 ccggccgcca cggtggcggt gccctgcagc acgttggtga cgaggctttc cttgtcgatg
     4141 gcgtagttca ccgcctggcg catccgcacg tcgtcgaagg gcgcttccct ggtgttgagg
     4201 atgaggaacc acaaatgcgg gccgg
//
LOCUS       DAOTFI010000327         4207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_25001_length_4207_cov_6.546724, whole genome shotgun sequence.
ACCESSION   DAOTFI010000327 DAOTFI010000000
VERSION     DAOTFI010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25001_length_4207_cov_6.546724"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..742)
                     /locus_tag="P5558_22295"
     CDS             complement(8..742)
                     /locus_tag="P5558_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRY27111.1"
                     /translation="MPQLTLPGTSEAHPIEACVFDAYGTLLDVHSALASVLQARAGQA
                     GPAIDDARAKSLSILWRDKQLACSWLRSLMRHHADFASVTADALDFALSVHGLDGDPT
                     LRAELLAAYDKLAPYPEVPGVLARLAEGGLPLAVLSNGTPAMLDAGLAHAGIAGSFTA
                     ILSIEEIGVFKPAPEVYSLATRHLGLAAPKILFLSSNGWDVHGAAAFGFTTIHVDRQA
                     SPPERLPGRPVHRLADLAPLPGLLGL"
     gene            880..1059
                     /locus_tag="P5558_22300"
     CDS             880..1059
                     /locus_tag="P5558_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HRY27112.1"
                     /translation="MNLDFTPGAVVRLRTKPDWGLGRVQSAIGPKVTVDFDEAGKVVI
                     DLRHAALELVTPAEG"
     gene            complement(1067..2230)
                     /locus_tag="P5558_22305"
     CDS             complement(1067..2230)
                     /locus_tag="P5558_22305"
                     /inference="COORDINATES: protein motif:HMM:NF018549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27113.1"
                     /translation="MLHAVVDWQRWLLEPWLTGLRMGAGQPLWQRLLAAQHVLAERTM
                     AASEAGTEGIETAVARDHAGVSSAHTVEAGPFSRLVRFRAGGSGPPTLLVAPHSGYAG
                     AVLSELAASLLQHGDVYFLDWLDARLVPKSRGRFGLDEQLAVVLKALVTIGSPAHLVG
                     VSQSGPAALAAAALATNASATLPRPRSLVLLGAPVGPAERRSAIDWLAAGLDPGIVDT
                     CLISVVPERYPGAGRRVYPGLFQLMAVLFADPGAYFGAQAGLWLELMAGAPGPCDRLH
                     ADLHRLVDVPAELYAESVDNLVRRSPFDGSGFTLGSHAIPTGDLAGLPVLTVEAAKDE
                     LVGAGATHAVQALAGPGSAELTVHDAAHHALFVGPLFASAVAPPLRGFLQRQR"
     gene            complement(2269..>4207)
                     /gene="alaS"
                     /locus_tag="P5558_22310"
     CDS             complement(2269..>4207)
                     /gene="alaS"
                     /locus_tag="P5558_22310"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRY27114.1"
                     /translation="LQGKHDNYDIDLFQKLIAHSVELTGTPATGPTAPSHKVIADHLR
                     AASFLIADGVTPDNDGRGYVLRRIMRRGMRHAHLLGATEPLMHRLAPVLVEEMGRAFP
                     ELVRAEASIVEWLKLEETNFRRTLERGLRLLDEATADLPQGQALAGETAFKLYDTYGF
                     PLDLTEDALRATGRTVDHEGFEAAMERQRADARRHWKGSGAEAVAPVWFEIREAHGKT
                     EFLGYATTKADARVLALVVDGSPVDAVEPGPEVEIVTNQTPFYGESGGQLGDTGWLLG
                     QGLEAVVSDTTRRLGDLWVHHARLTRGRLAVGDSVQLQVDADRRDRLRRAHSATHLLH
                     AALRHRLGDHVAQKGSLVAPDRLRFDFSQPRAMTPEDIRVVEREVNAYVQQNAPVEVR
                     VMARDEAIARGAMALFGEKYGDEVRVVTMGREPDGDTYSMELCGGTHVARTGDIALAK
                     LVAESAVAAGVRRVEALTGAAALAHIETEEGWLAEAAEALKVQPAGLADRVRDLVAEK
                     RRLEQELAELRRKLATGASDAGPARDIAGIAFTARALDGVPAKELRTAADRLKQDMKS
                     GVVALVAVNDGKAAVVVSVSEDLTGRIDAVDLVRQGVAALGGKGGGGRADFAQGGGPD
                     GAEAAAALAAIEQRLAELAVG"
BASE COUNT          552 a         1327 c         1768 g          560 t
ORIGIN      
        1 gggatgtcta gaggccgagg aggccgggga gcggggcgag gtcggcgagg cggtggaccg
       61 ggcggccggg caggcgttcg ggcgggctgg cctggcggtc gacgtggatg gtggtgaagc
      121 cgaaggcggc cgccccgtgc acgtcccagc cgttcgagga gaggaagagg atcttcggtg
      181 cggcgaggcc gaggtggcgg gtggcgaggc tgtagacctc gggcgcgggc ttgaagacgc
      241 cgatctcctc gatggagagg atggcggtga acgagccggc gatgccggca tgggcgaggc
      301 cggcatcgag catggcgggg gtgccgttgg agaggacggc gagcggcagg cctccctcgg
      361 cgaggcgggc caggacgccc ggcacctcgg gatagggggc gagcttgtcg taggcggcga
      421 gcagctcggc gcggagcgtc gggtcgccgt cgaggccgtg cacggaaagc gcgaagtcga
      481 gggcgtcggc ggtgacgctg gcgaagtcgg cgtggtggcg catgagcgag cggagccagg
      541 aacaggcgag ctgcttgtcg cgccagagga tggagaggct tttcgccctg gcgtcgtcga
      601 tggcggggcc ggcctgccct gccctcgcct gcagcaccga ggccagggcg gagtggacgt
      661 cgaggagggt gccgtaggcg tcgaagacgc aggcttcgat ggggtgggcc tcgctggtgc
      721 ccggaagggt gagctgtggc atgaccgatg gctgtcgcac cggggccggg attggtcaag
      781 tcgccgccag tcatcagggg ggggccggtc ttgatcccgt gccggtggcg gcggatattg
      841 cctggatcgc cggggtgtcc cggcagggat ggggatgcca tgaatctgga cttcacgccg
      901 ggtgcggtcg tccggctgcg gacgaagccc gactgggggc tgggccgggt gcagtcggcc
      961 atcggtccca aggtcacggt ggatttcgac gaggccggca aggtggtcat cgatctgcgg
     1021 cacgccgccc tggagctggt gacgccggcc gaggggtgag agccggtcag cgctgccgct
     1081 ggaggaagcc gcggagtggc ggggccacgg cgctggcgaa gagggggccg acgaagaggg
     1141 cgtggtgggc ggcgtcgtgg accgtgagct ctgccgagcc ggggccggcg agcgcctgca
     1201 cggcatgggt ggcgccggcg ccgacgagct cgtccttcgc cgcctcgacg gtcaggaccg
     1261 gcaggccggc gaggtcgccg gtcgggatgg cgtggctgcc gagggtgaag cccgagccat
     1321 cgaacggcga gcggcggacc aggttgtcga cgctttcggc gtagagctcg gccggcacgt
     1381 cgaccagccg gtgcaggtcg gcgtgcagcc ggtcgcaggg gccgggcgcg ccggccatga
     1441 gctcgagcca gaggccggcc tgggcgccga aataggcgcc aggatcggcg aagagcaccg
     1501 ccatgagctg gaagagcccc ggatagacgc ggcggcccgc ccccggatag cgttccggca
     1561 cgaccgagat caggcaggtg tcgacgatgc cgggatcgag cccggcggcg agccagtcga
     1621 tggcggagcg gcgctcggcc ggcccgacgg gggcacccag cagcacgagc gagcgcggcc
     1681 ggggcagggt cgccgaggcg ttcgtggcga gggcggcggc ggcgagggcg gcggggccgg
     1741 actgggagac gccgacgagg tgggccgggc tgccgatggt gaccagggcc ttgagcacta
     1801 cggcgagctg ctcgtcgagg ccgaagcggc cgcggctctt gggcacgaga cgggcatcga
     1861 gccagtcgag gaagtagacg tcgccgtgct gcaggaggct ggcggcgagc tcgctcagca
     1921 ccgcgccggc atagcccgaa tgcggggcga cgaggagggt gggggggccg ctgccgccgg
     1981 cgcggaagcg gacgaggcgc gagaacgggc cggcctcgac ggtgtgggcg ctcgagacgc
     2041 ccgcatggtc gcgggcgacg gccgtctcga ttccctcggt gccggcctcg cttgccgcca
     2101 tggtgcgctc ggcgagcacg tgctgggccg cgagcaggcg ctgccagagc ggctggccgg
     2161 cgcccatccg gagcccggtg agccagggct cgagcagcca gcgctgccag tcgacgacgg
     2221 catggagcat gctagccgat cagacgctcg gggggtggcg cggggcgctc agccgaccgc
     2281 aagctcggcc agtctttgct cgatcgcggc cagcgccgct gccgcttcgg cgccgtcggg
     2341 gccgccgcct tgcgcgaagt cggcgcggcc gccgccgcct ttgccgccga gtgccgcgac
     2401 accttgcctg acgaggtcga ccgcgtcgat gcggccggtc aggtcctcgc tgacgctgac
     2461 gacgacggcg gccttgccgt cgttgacggc gaccagcgcc accacaccgc tcttcatgtc
     2521 ctgcttcagc cggtccgccg cggtgcgcag ctccttggcg ggaacgccgt cgagggcgcg
     2581 cgcggtgaag gcgatgccgg cgatgtcgcg ggccggcccg gcgtcgctgg cgccggtggc
     2641 gagcttgcgg cggagctccg ccagctcctg ctccagcctg cgcttctcgg ccacgaggtc
     2701 gcgcacccgg tcggcgaggc cggcgggctg gaccttgagc gcctcggccg cctcggcgag
     2761 ccagccctcc tcggtctcga tatgggcgag ggcagcggcg ccggtcagcg cctcgacgcg
     2821 gcgcacgccg gcggcgacgg cgctctcggc gaccagcttg gccagcgcga tgtcgccggt
     2881 gcgggcgaca tgcgtgccgc cgcagagctc catcgagtag gtgtcgccgt cgggctcccg
     2941 gcccatggtg acgacgcgga cctcgtcgcc gtacttctcg ccgaagagcg ccatggcccc
     3001 gcgggcgatg gcctcgtcgc gggccatgac gcggacctcg accggggcgt tctgctggac
     3061 gtaggcgttg acctcgcgct cgaccacgcg gatgtcctcg ggcgtcatgg cgcgcggctg
     3121 gctgaagtcg aagcgcagcc ggtcgggggc gaccagcgag cccttctgcg ccacgtggtc
     3181 gccgaggcga tggcggaggg cggcgtgcag caggtgggtg gcggagtgcg cccggcgcag
     3241 gcgatcgcga cggtcggcat cgacctggag ctggacgctg tcgccgacgg cgaggcggcc
     3301 cctggtcagc ctcgcgtggt ggacccagag gtcgccgagg cgcctggtgg tgtcgctcac
     3361 caccgcctcg aggccctggc cgaggagcca gcccgtgtcg ccgagctggc cgccggattc
     3421 gccgtagaag ggggtctggt tggtgacgat ctcgacctcg gggccgggct ccaccgcatc
     3481 gaccgggctg ccgtcgacga ccagcgcaag gacgcgggcg tcggccttcg tggtggcgta
     3541 gcccaggaac tcggtcttgc cgtgggcttc gcggatttcg aaccagacgg gggccacggc
     3601 ctcggcaccg gagcctttcc agtgccgccg ggcgtcggcg cgctggcgct ccatggcggc
     3661 ctcgaagccc tcgtggtcga cggtgcgacc ggtggcgcgc agcgcatcct cggtgaggtc
     3721 gagcgggaag ccgtaggtgt cgtagagctt gaaggcggtc tcgccggcga gggcctggcc
     3781 ctggggcagg tcggcggtcg cctcgtcgag caggcgcagg ccacgctcga gggtgcggcg
     3841 gaaattggtc tcctcgagct tcagccactc gacgatcgag gcctcggcgc ggaccagctc
     3901 ggggaaggcg cggcccatct cctcgaccag gacgggggcg aggcgatgca tgagcggctc
     3961 cgtggcgccg agcaggtgag cgtggcgcat gccgcggcgc atgatgcggc ggagcacgta
     4021 gccgcggccg tcattgtcgg gggtgacgcc gtcggcgatg aggaaggagg cggcccggag
     4081 gtggtcggcg atgaccttgt gggagggggc cgtcgggccg gtcgccgggg tgccagtcag
     4141 ctcgacgctg tgggcgatga gcttctggaa aaggtcgatg tcgtagttgt cgtgcttgcc
     4201 ctggaga
//
LOCUS       DAOTFI010000328         4121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_25671_length_4121_cov_7.023856, whole genome shotgun sequence.
ACCESSION   DAOTFI010000328 DAOTFI010000000
VERSION     DAOTFI010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25671_length_4121_cov_7.023856"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="P5558_22315"
     CDS             <1..890
                     /locus_tag="P5558_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27115.1"
                     /translation="RAVGLMALLSLAACATGTPRPVADGDPVEPPMVEMVAPLDPELR
                     GRLARSALLVNAMPAVNAANWRPPRRQPEPPGFFEGLFYTPLGFIIVLPLIPVVAGVL
                     AYHDLSDHPAEHHVAIRDIDLATSFLASLDDDVPKRELSVSTVCPANTSVAACQGHVA
                     GAADLLIRVYLQTQYLGSSKQLPNGALAISMSTLISPIEDGTISASRYQMHWRWRAPQ
                     PSSTNAAATGPSAFRAAVDAGLGRLARTLEDDLWRRVQPVALPAAVAGQLPAQQIFSQ
                     PPGSIVPAELRPLWLTVLE"
     gene            924..1577
                     /gene="coaE"
                     /locus_tag="P5558_22320"
     CDS             924..1577
                     /gene="coaE"
                     /locus_tag="P5558_22320"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462961.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRY27116.1"
                     /translation="MSPLVIGLTGGIAMGKSRVARLLQGFGVPVFDADAAVHALLRAG
                     SPVVGAVEAAFPGVGDDAGGIDRKALGARVFGDLPALRRLEAILHPAVRAAERRFLGR
                     LARQRFAMAALDIPLLLETGAERLCDCVVVVDAGPELQAARALARPWMSRARLAAIRA
                     QQMPSAEKQKRADFVLRSGYDRGASAAAVAALLDRLEGWPRRAWPGRWKGAARVEGH"
     gene            1577..2245
                     /gene="dnaQ"
                     /locus_tag="P5558_22325"
     CDS             1577..2245
                     /gene="dnaQ"
                     /locus_tag="P5558_22325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRY27117.1"
                     /translation="MREIVMDTETTGLDPATGHRIVEIGAIELEHHVPTGRHFHCYIN
                     PERDMPEDAFRVHGLSSEFLAEHPVFAAIARDFLAFIGEAPLVIHNASFDLRFLNAEL
                     GRLDLPLLPASRAIDTLPMAQRKFPGSPANLDALCRRFGVDLQARELHGALLDSRLLA
                     EVYVHLKGGLQANLGLTLQARGGRAGGMAAERRPHRVFVVPPEELAAHEAFVAAMKDP
                     VWRR"
     gene            2340..3557
                     /locus_tag="P5558_22330"
     CDS             2340..3557
                     /locus_tag="P5558_22330"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRY27118.1"
                     /translation="MGKIKVAKPIVEIDGDEMTRIIWQMIKDKLILPYLDIELLYFDL
                     GIEHRDATNDQVTIDAAEAIKKHNVGVKCATITPDEARVEEFGLKKMWRSPNGTIRNI
                     LGGTVFREPIIMRNVPRLVPGWTQPIVIGRHAFGDQYRATDFKVPGKGRLTMRFEPEG
                     GGEAVEHEVFQFPAAGVAMGMYNLDESIREFARASLNYGLMRKWPVYLSTKNTILKVY
                     DGRFKDLFEEVFEAEFKSDFEKAGITYEHRLIDDMVASALKWSGGFVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGKATSTNPIASIFA
                     WSRGLAYRAKIDGSDALAAFARTLEEVCVETVEGGKMTKDLALLIGPDQPWLTTEQFL
                     DALDEGLKAKMGA"
     gene            complement(3596..>4121)
                     /locus_tag="P5558_22335"
     CDS             complement(3596..>4121)
                     /locus_tag="P5558_22335"
                     /inference="COORDINATES: protein motif:HMM:NF017119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HRY27119.1"
                     /translation="RFVWRSEAEYATDEDAFEQLENQARVTQPISEFFDLAYGLRLDT
                     PEGATRLHGLIGIQGLAPQWFEIDATLFVSDTPFARFETEYEVLITNRLNLVPSLELT
                     LPMVDDEGIGAGAWGPKLEMGLRLGYDLIDRSFTPYIGVHYERLFGQTADYARDDGEE
                     RDALFFVVGARLLF"
BASE COUNT          632 a         1401 c         1447 g          641 t
ORIGIN      
        1 cccgagccgt cgggctcatg gcgctgctgt cgctcgccgc ctgcgcgacg ggcacgccgc
       61 ggccggtcgc ggacggcgat ccggtcgagc caccgatggt cgagatggtg gcacctctcg
      121 atccggagct gcgcggccgg ctcgcgcgat cggccttgct cgtgaacgcg atgccagccg
      181 tcaatgccgc gaactggcgc ccacctcgcc gacagccgga gccgccgggc ttcttcgagg
      241 ggctgttcta tacgcctctg gggttcatca tcgtcttgcc gctgataccg gtggtggcag
      301 gcgtcctcgc ctaccatgat ttgtccgacc atccggccga acatcacgtc gcgatccgtg
      361 atatcgatct cgccacgtcc ttcctcgcga gcctggatga tgacgtgccg aaaagggagc
      421 tctcggtctc gaccgtctgt ccggccaaca ccagcgtggc tgcttgccaa ggacatgttg
      481 cgggtgcggc cgacctgttg atcagggtct acttgcagac gcagtatctc ggctcctcga
      541 agcaactgcc gaacggcgcc ttggccatca gcatgtcgac cctgatttcc ccgatcgagg
      601 acgggacgat cagcgcctcg cgctatcaga tgcattggcg ctggcgtgcg ccacagccat
      661 cgtcaacgaa cgccgccgcg acggggccgt cggcgtttcg ggcagccgtc gacgccgggc
      721 tgggccggct ggcccgaacg ctcgaggatg acctctggcg gcgcgttcag ccggtggccc
      781 tgccggccgc cgtggccggg caactgccgg cgcagcagat tttcagccag ccacccggca
      841 gcatcgtccc ggcagagctg cggccgctct ggcttacggt gctggagtag ctgcaccgtg
      901 cccacccgag acgagccctt cgtatgagcc cgctggtcat cggcctcacc ggcggcatcg
      961 ccatgggcaa gagccgggtc gcccggctgc tccagggctt cggggtgccg gtcttcgatg
     1021 cggatgccgc ggtgcatgcg ctgctgcggg ccggcagccc ggtcgtcggc gcggtcgagg
     1081 cggcgttccc cggggttggg gacgatgccg gcgggatcga ccggaaagcg ctcggcgcca
     1141 gggtcttcgg cgatttgccg gcgctccgcc ggctcgaggc gatccttcac ccggcggttc
     1201 gcgccgccga gcggcggttt ctcggccggc tcgcccggca gcgcttcgcc atggcggcgc
     1261 tggacatccc gctcctgctc gagacggggg ccgagcggct ctgcgactgc gtcgtcgtgg
     1321 tcgatgcggg gccggagctg caggcggcgc gggccctggc acggccgtgg atgagccggg
     1381 cgcggctggc ggcgatccgg gcacagcaga tgccaagtgc ggagaagcag aagcgggcgg
     1441 acttcgtcct gcgttccggc tatgaccggg gggcgagcgc cgctgccgtg gcggcgctgc
     1501 tcgaccggct ggagggctgg ccgcggcggg cctggcccgg gcgctggaag ggtgctgccc
     1561 gagtggaggg gcattgatgc gcgagatcgt catggacacg gagacgaccg gcctcgaccc
     1621 ggccacgggc caccggatcg tcgagatcgg ggcgatcgag ctcgaacacc acgtgccgac
     1681 cggccggcat ttccactgct acatcaaccc cgagcgcgac atgcccgagg acgcctttcg
     1741 cgtgcacggg ctgagcagcg agttcctcgc cgagcatccc gtgttcgcgg cgattgcccg
     1801 ggacttcctg gcgttcatcg gcgaggcgcc gctggtcatc cacaatgcca gcttcgacct
     1861 gcgctttctc aatgccgagc tcggccggct cgacctgccg ctcctgccgg cgagccgggc
     1921 gatcgacacg ctgccgatgg cgcagcgcaa gttcccgggc tcgcccgcca acctcgacgc
     1981 gctctgccgc cgtttcggtg tcgatctcca ggcccgcgag ctgcacggcg cgctcctcga
     2041 cagccggctg ctcgccgagg tctacgtgca tctcaagggg gggctgcagg ccaatctggg
     2101 cctcaccctg caggcgcggg gcgggcgggc gggcggcatg gcggcggaac ggcggccgca
     2161 tcgcgtcttc gtcgtgccgc ccgaggagct ggccgcccac gaggccttcg tcgccgcgat
     2221 gaaggatccc gtctggcggc gctagccggg ccgcactgcg accccgtggg cgctttgcgc
     2281 tggcttgcgg cctcgactac aaggccggcc atgcagtgtg cagggccgag gggatacgga
     2341 tgggcaagat caaggtagcg aagccgatcg tcgagatcga cggcgacgag atgacccgga
     2401 tcatctggca gatgatcaag gacaagctga tcctgcccta tctcgacatc gagctgctct
     2461 acttcgatct cggcatcgag caccgcgacg ccacgaacga ccaggtgacg atcgacgccg
     2521 cggaggcgat caagaaacac aatgtcggcg tcaagtgcgc caccatcacg ccggacgagg
     2581 cccgggtcga ggagttcggc ctgaagaaga tgtggcggtc gcccaacggc acgatccgca
     2641 acatcctggg cggcacggtc ttccgcgagc cgatcatcat gaggaacgtg ccgcggctgg
     2701 tgccgggctg gacccagccc atcgtcatcg gccgtcatgc cttcggcgac cagtatcggg
     2761 ccacggactt caaggtgccg ggcaagggca ggctcaccat gcgcttcgag cccgagggcg
     2821 gcggcgaggc ggtcgagcac gaggtcttcc agttcccggc ggccggcgtc gccatgggca
     2881 tgtacaatct cgacgagagc atccgcgagt tcgcccgggc gtcgctgaac tacggcctga
     2941 tgcgcaagtg gccggtctat ctctcgacca agaacaccat cctcaaggtc tatgacggcc
     3001 gcttcaagga cctcttcgag gaggtcttcg aggccgagtt caagagcgac ttcgagaagg
     3061 ccggcatcac ctacgagcac cggctgatcg acgacatggt ggcctcggcg ctcaagtggt
     3121 cgggcggctt cgtctgggcc tgcaagaact acgacggcga cgtgcagtcc gacaccgtgg
     3181 cccagggctt cggctcgctc ggcctcatga cctcggtcct gatgaccccg gacggcaaga
     3241 cggtcgaggc cgaggcggcg cacggcacgg tgacccgcca ctaccgcgag caccagaagg
     3301 gcaaggccac ctcgaccaac ccgatcgcct cgatcttcgc ctggagccgg ggcctcgcct
     3361 accgggcgaa gatcgacggc tcggacgcgc tcgccgcctt cgcgaggacg ctcgaggagg
     3421 tctgcgtcga gaccgtcgaa ggcggcaaga tgaccaagga cctggccctg ctcatcggcc
     3481 cggaccagcc ctggctgacc accgagcagt tcctcgatgc gctggacgag gggctgaagg
     3541 ccaagatggg cgcctgaatg aagtcctggc cgccgtcagg cggccgggga cagggtcaga
     3601 acagcaggcg cgcgccgacg acgaagaaga gggcgtcgcg ctcctcgccg tcgtcacggg
     3661 cgtagtcggc ggtctgaccg aagagccgct cgtagtggac gccgatatag ggcgtgaagg
     3721 agcggtcgat caggtcgtag ccgaggcgta accccatctc cagctttggg ccccaggcgc
     3781 cggccccgat cccctcgtcg tccaccatgg gcagcgtcag ctcgaggctc ggcaccaggt
     3841 tcaggcggtt ggtgatcagc acctcgtact ccgtctcgaa gcgcgcgaac ggcgtgtccg
     3901 agacgaagag cgtggcgtcg atctcgaacc attgcggcgc caggccctgg atgccgatca
     3961 ggccgtgcag gcgtgtggcc ccctccggcg tgtccaggcg cagcccgtag gcgaggtcga
     4021 agaactcgct gatcggctgc gtgacccggg cctggttctc gagctgctcg aaggcgtcct
     4081 cgtcggtcgc gtactcggcc tcgctgcgcc agacgaagcg c
//
LOCUS       DAOTFI010000329         4103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_25838_length_4103_cov_5.531126, whole genome shotgun sequence.
ACCESSION   DAOTFI010000329 DAOTFI010000000
VERSION     DAOTFI010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25838_length_4103_cov_5.531126"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="P5558_22340"
     CDS             complement(<1..337)
                     /locus_tag="P5558_22340"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRY27120.1"
                     /translation="MTSQTAHVAPPAQAAQRAVAAGKVALVTGITGQDGAWLAELLLA
                     KGYIVHGLKRRASSFNTARIDHLYQDPHEQGRRLVLHYGDLTDATNLIRVVQEVRPDE
                     IYNLAAQSHV"
     gene            complement(334..1305)
                     /locus_tag="P5558_22345"
     CDS             complement(334..1305)
                     /locus_tag="P5558_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY27121.1"
                     /translation="MTLDNQRILVTGGAGFVGSHLCERLLDAGHEVLAVDNHFTGRRA
                     NVAHLLGHPRFELMRHDITFPLYVEVDAIYNLACPASPIHYQRDPVQTTKVSVIGAIN
                     MLGLAKRTGARILQASTSEVYGDPDIHPQPETYRGNVNPIGPRACYDEGKRCAETLFF
                     DYRRQHGLKIKVARIFNTYGPRMQPNDGRVVSNFIVQALQGAPITLYGDGRQTRSFCY
                     VDDLVDGLIRFMATPDHVTGPVNLGNPGEFTIRQLAEQVLHLTGSRSTLVNEPLPEDD
                     PMQRQPDITLARELLDWQPAIPLEEGLKRTIPYFKELLSMEAKQVRA"
     gene            complement(1388..2851)
                     /locus_tag="P5558_22350"
     CDS             complement(1388..2851)
                     /locus_tag="P5558_22350"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRY27122.1"
                     /translation="MSVALFARYPISRQTLVVLVDAAAITVALPLALVLRENRLPAGD
                     RLAELWASLPLLALAAVTASLLLGSYRAVWRYMGIAEIVRLAQLALLAILLFYLGQFV
                     VDRLEHLPRSAPPIHFLVAMFLLIGARILYGEWCRRGHGRAGGPTRRPLLLVGSGDGA
                     ALFIQMLQHQPDRQYDIAGIICDQVARHRSIAGVPVLGGLADFDAVLATLRVQGMTPD
                     RVVVTRPHHELGRDAVYHIMSRADAQGIAVEQLPDLMRFRADTPVAPRAAPLAAPLSD
                     GLPEAVYPRLKRLFDIALSATVLVILAPLLLVAAVAVALFIQRPVMFVQIRPGLHRRP
                     FRLYKLRTMQDPLGPGGRRLADAERTPFVGRLLRRTRLDELPQLWNVLIGDMAIIGPR
                     PLVAADLDAMADSGRARSRARPGITGWAQVNGGQQLDADEKLALDLWYADHASLALDL
                     RIIGRTLHMMLMGERRDPSAIDTARAALVPEVMGAAE"
     gene            complement(3041..>4103)
                     /locus_tag="P5558_22355"
     CDS             complement(3041..>4103)
                     /locus_tag="P5558_22355"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRY27123.1"
                     /translation="DNVRLSQAEVGLREREREAEALRNLYNSFLARFTETREQRQVIE
                     ADARIIARAESPQRPSSRGLEFFLAIGFVVSGAAGVGLAWLRETLDRGLRSGGELERQ
                     LGLPCLGQVPHLKGIAGKGTKPHLYLLDKPRSAYAESIRSVGTYLRMSNIDDPPRVIQ
                     ITSAIPGEGKTTFAASLATLLAKSGQQTVLIDLDLHHPSVARELGLEVEHCLVDYMLG
                     DAALEDVIHTTEFGLAVLPVRRQTADPSVLVGSERLKQLLATMRERFDYVVVDSPPLL
                     LVTDPKTTSAQVDASVLLVRWQETPADKAANALRELDAVAARVAGAVLAQVDVKRQAQ
                     YGYAGVGSYYGDYKKYYVD"
BASE COUNT          657 a         1166 c         1646 g          634 t
ORIGIN      
        1 tgacgtggct ctgggcggcg aggttgtaga tttcgtcggg gcggacctcc tggacgacgc
       61 ggatcaggtt ggtggcgtcg gtgaggtcgc cgtagtggag gacgaggcgg cggccttgtt
      121 catgcgggtc ctggtagagg tggtcgatgc gggcggtgtt gaaggaggag gcgcggcgct
      181 tgaggccgtg gacaatgtag cccttggcga gcaggagctc ggcgagccag gcgccgtcct
      241 ggccggtgat gccggtgacg agggccactt taccggcggc taccgcgcgc tgcgctgctt
      301 gagccggggg cgctacatga gccgtttggg aggtcatgcc ctcacctgtt tcgcttccat
      361 cgacaagagt tccttgaaat aggggatggt gcgcttcagg ccttcctcga gcgggattgc
      421 aggctgccag tcgagcagct cgcgggcaag ggtgatgtcg ggctggcgct gcatggggtc
      481 gtcctcgggg agcggctcgt tgaccaaggt cgagcgggag ccggtgaggt ggaggacctg
      541 ctcggcgagc tggcggatgg tgaactcgcc ggggttgccg aggttgacgg ggccggtgac
      601 gtggtcgggg gtggccatga agcggatcag gccgtcgacc aggtcgtcga cgtagcagaa
      661 ggagcgggtc tggcggccgt cgccgtagag ggtgatgggg gcgccttgga gcgcctggac
      721 gatgaagttg gagacgacgc ggccgtcgtt cggctgcatg cgcgggccgt aggtgttgaa
      781 gatgcgggcg accttgatct tcaggccgtg ctggcggcgg tagtcgaaga agagggtctc
      841 ggcacagcgc ttgccttcgt cgtagcaggc gcgcgggccg atggggttga cgttgccgcg
      901 gtaggtctcg ggctgcgggt ggatgtcggg gtcgccgtag acctcactgg tgctggcctg
      961 gaggatgcgg gcaccggtgc gcttggcgag gccgagcatg ttgatggcgc cgatgaccga
     1021 gaccttggtc gtctggacgg ggtcgcgctg gtagtggatc ggcgaggccg ggcaggccag
     1081 gttgtagatg gcgtcgacct cgacgtagag ggggaaggtg atgtcgtggc gcatcagctc
     1141 gaagcggggg tggcccagca ggtgcgccac gttggcgcgg cggccggtga agtggttgtc
     1201 gacggccagg acctcgtggc cggcgtcgag caggcgctcg cagaggtggc tgccgacgaa
     1261 gccggcgccg ccggtgacca ggatgcgctg gttgtcgagg gtcaattggc ggccccgcat
     1321 ggctggggca gcggcgctgc ccgggcggtg accaggatgc gctggttgtc gagggggttg
     1381 tcgagggtca ttcggcggcc cccatgactt ccggcacgag ggctgcccgg gcggtgtcga
     1441 tggcggaagg atcgcggcgc tcgcccatga gcatcatgtg gagggtgcgg ccgatgatgc
     1501 gcagatcgag ggcgaggctg gcgtggtcgg cgtaccagag gtcgagggcg agcttctcgt
     1561 ccgcgtcgag ctgctggccg ccattgacct gggcccagcc ggtgatgccc gggcgggcgc
     1621 gggagcgggc ccggccgctg tccgccatgg catcgaggtc ggcggcgacg agcgggcgcg
     1681 ggccgatgat cgccatgtcg ccgatcagca cgttccagag ctgcggcagc tcgtcgaggc
     1741 gggtgcggcg gagcaggcga ccgacgaagg gcgtacgctc ggcgtcggcg aggcggcggc
     1801 cgccggggcc cagcgggtcc tgcatggtgc gcagcttgta gagccggaac gggcggcggt
     1861 gcaggcccgg gcggatctgc acgaacatga ccgggcgctg gatgaagagc gccacggcga
     1921 cggcagcgac gagcaggagg ggggccagga tgaccagcac ggtggccgag agggcgatgt
     1981 cgaaaagccg cttgagcctg ggatagacgg cttcggggag gccatcggac aggggtgccg
     2041 ccaatggcgc ggcgcggggc gcaaccgggg tatcggcgcg aaagcgcatc aggtccggga
     2101 gctgctcgac ggcaatgccc tgggcgtcgg cccggctcat gatgtgatag acggcgtcac
     2161 ggccgagctc gtggtgcggg cgggtcacga ccacgcgatc cggggtcatg ccctggacac
     2221 ggagcgtggc gagcacggca tcgaagtcgg cgaggccacc caggaccggc acgccggcga
     2281 tgctgcggtg gcgggcgacc tgatcgcaga tgatgccggc aatgtcgtac tggcggtcgg
     2341 gctggtgctg gagcatctgg atgaagaggg ccgcaccgtc acccgagccc accaggagca
     2401 gcggccggcg cgtcgggccg ccggccctgc cgtggccgcg ccggcaccat tcgccataga
     2461 ggatgcgcgc gccgatcagc aggaacatgg cgacgaggaa atggatgggc ggggccgagc
     2521 ggggcaggtg ctcgagccgg tcgacgacga actggccgag atagaagagg aggatggcga
     2581 ggagggcgag ctgggcgagg cggacgatct cggcgatgcc catgtagcgc cagacggcgc
     2641 ggtagctgcc gagcagcagg ctggcggtga cggcggcgag ggcgagcagc ggcaggctgg
     2701 cccaaagctc ggcgaggcgg tcgccggcgg gcagccggtt ctcgcgcagc accagcgcca
     2761 gcggcagggc cacggtgatc gccgccgcgt cgaccaggac gacgagcgtc tgacggctga
     2821 tcgggtagcg cgcgaacaag gcgaccgaca agaacagctc ccctccgaga gaaggctcat
     2881 atctcctgat tggccgagca ctgatttcag cacttaccga taacagagtt gtgatgagag
     2941 gcaacatgca agtgaggcga acaaggaaga tattgctcta tctcaagttg agaacattct
     3001 cctgaaaaat caacagtttc tggtcaccgt ctgcgtttcg tcaatcgacg taatatttct
     3061 tatagtcacc atagtacgag ccgaccccag catagccgta ttgcgcctgg cgcttcacgt
     3121 cgacctgggc gagcacggcg ccggcgaccc tggcggcgac ggcatcgagc tcgcgcagcg
     3181 cattggcagc cttgtccgcg ggcgtctcct gccaacggac gagcagcacg ctggcatcga
     3241 cctgagcact cgtggtcttc gggtcggtga cgagcagcaa gggtgggctg tcgacgacca
     3301 cgtagtcgaa gcgctcgcgc atcgtggcca ggagctgctt gaggcgctcc gagccgacga
     3361 gcaccgaggg gtcggcggtc tggcggcgaa ccggcaggac ggcgaggccg aactcggtcg
     3421 tgtggatgac gtcctcgagg gcggcgtcgc cgagcatgta gtcgacgagg caatgctcga
     3481 cctcgaggcc gagctcgcgg gcgacagatg gatggtgcag gtcgagatcg atcagcacgg
     3541 tctgctgccc cgacttggcg agcagcgtgg cgaggctggc ggcgaaggtg gtcttgcctt
     3601 cacccggaat cgccgaggtg atctggatga cgcgcggcgg gtcgtcgata ttgctcatgc
     3661 ggagataggt cccgaccgag cggatcgatt cggcataggc cgagcgcggc ttgtcgagca
     3721 gatagagatg cggcttcgtg cccttgccgg cgatcccctt gaggtgggga acctgaccga
     3781 ggcagggcag gccgagctgg cgttcgagct cgccgccgct cctgaggccg cgatccaggg
     3841 tctcgcgcag ccaggcgagg ccgacgccgg cggcacccga aaccacgaag ccgatcgcca
     3901 ggaagaactc gaggccgcgc gagctcggtc gttgcgggct ctccgcgcgg gcgatgatgc
     3961 gggcatcggc ctcgatcacc tggcgctgct cacgtgtctc ggtgaagcgc gccaggaagg
     4021 agttgtagag gttgcggagc gcttcggcct cgcgctcgcg ctcgcgcagg ccgacctcgg
     4081 cctggctcag gcggacattg tcc
//
LOCUS       DAOTFI010000330         4101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_25854_length_4101_cov_6.529659, whole genome shotgun sequence.
ACCESSION   DAOTFI010000330 DAOTFI010000000
VERSION     DAOTFI010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25854_length_4101_cov_6.529659"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2113)
                     /gene="priA"
                     /locus_tag="P5558_22360"
     CDS             complement(<1..2113)
                     /gene="priA"
                     /locus_tag="P5558_22360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRY27124.1"
                     /translation="MTLATTSVLLPMPLPTCFDYRLEGELATTPPPLGSYVVVPFGPR
                     RVVGVVWERGSARPVAEARLKPVQAVLGQPPLPAPLRRLVEAVARATVTPMGSVLRLV
                     LSTPAAFEAEPARIVLVPTGAAVPLSAKQQLVLAAFATGGPQPAAALARAAGVSAGVV
                     KTLVDKGVLRSETVAQGLPDPPCYQPPDPARLTPAQAQATAALLAAIADGSGGEVLLE
                     GLPGAGKTEIYLEAIAATLARGRSALVLLPEIALGAQWLDRFTSRFGAEPLLWHSGLT
                     ALQRRRAWKLAAAGRAPVVVGARSALFLPMPELGLVVIDEEHDTSFKQEDGVIYDARD
                     VARRRAALQDARLVTCSATPSLETQGLARTHITVEGRFAAQSKPAMALVDLRRDKPPK
                     GAFLAPVLRQALAAALEQGEQAMLFLNRRGYAPLTLCRACGFRFRCPNCSAWLVAHRF
                     GQRLTCHHCGYGQHQPGHCPDCGAVDSLAVSGPGVERVQEEVKALFPEARTALMTSDT
                     VTSAARASSLVDAMLQRRIDILIGTQLIAKGHHFPDLTLVGVVDADIGLAGGDPRAAE
                     RSFSMLYQLAGRAGRESRPGRVLIQTYLPEHPVMEALAKGDRAAFMAAEREERELSGM
                     PPYGRLAALILAGPDAKLVKQEAQKLVRAAPDHEGWHILGPAPAPLALLRGRWRERIL
                     VKSPPDPELADSEWIFPTPELL"
     gene            2148..3125
                     /locus_tag="P5558_22365"
     CDS             2148..3125
                     /locus_tag="P5558_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRY27125.1"
                     /translation="MLASRGRHGKPHWRTVLLAFALPDLEEAFAAWCDWLAHERRAAA
                     RTQAGYRRDLAAFVAFLAGHQGGPVGLPAIAALTPQGFRGWLAWRLGEGYAKSSTQRA
                     VAAVRGFLDFLDARHGIHNPALRAMRAPKTRRPLPRPLAVAEVTELTDAAANADQPPW
                     VLARDTALLLLLYGAGLRIGEALELAAGGLPAAGESTLRVTGKGRKVRLVPLLPVVAE
                     ALAAYRDACPHPLPADGPLFRGVRGGPLQPAVVQRRVRELRRALGLPETATPHSLRHS
                     FATHLLGGGADLRSIQELLGHASLSTTQLYTAVDAEGLARLYTAAHPRA"
     gene            complement(3190..4044)
                     /locus_tag="P5558_22370"
     CDS             complement(3190..4044)
                     /locus_tag="P5558_22370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY27126.1"
                     /translation="MLASCSGGDNFRTLGTKFKNRGDAGETYAAATSATPPTGAFEKA
                     AYDGYVGHAKYEYESMQDYTDTIFHARRASAVAGGQTVAPQPTSERVLTPEHAAELSE
                     ARARYMAVAQGEPGQRLPAVSGRAMADYDCWIEQQEENFQPDDIAACRDAFYAGLAEL
                     EAPVVEEVDVISLSSDVLFEFDKYEVRPEFTAELDAAADTLLANPDVTIRIDGHTDSI
                     GTEEYNMGLSERRANAVADYLEAAGVARDRMTVAWFGESQPIASNDTAEGRALNRRVD
                     IDLIQVGD"
BASE COUNT          551 a         1362 c         1619 g          569 t
ORIGIN      
        1 ccaatagctc aggcgtgggg aagatccact cactgtcggc gagttcgggg tcgggggggg
       61 actttaccag gatgcgttcg cgccagcggc cgcggagcag ggcgaggggt gcgggggcgg
      121 ggccgaggat gtgccagccc tcgtggtcgg gggcagcgcg gacgagcttt tgcgcttctt
      181 gcttcacgag cttcgcgtcg gggccggcga ggatgagggc ggcgaggcgg ccgtaggggg
      241 gcatgccgga gagctcgcgc tcctcgcgct cggccgccat gaaggcggcg cggtcgccct
      301 tggcgagggc ttccatgacg gggtgctcgg gcagataggt ctggatcagg acgcggccgg
      361 ggcggctttc gcggccggcg cggccggcga gctggtagag catcgagaag ctgcgctcgg
      421 cggctctggg gtcgccgccg gcgaggccga tatcggcgtc gaccacgccg accagggtga
      481 ggtcggggaa gtggtggccc ttggcgatga gctgggtccc gatgaggatg tcgatgcgcc
      541 gctgcagcat ggcgtcgacg aggctcgagg ctctggcggc gctggtgacg gtgtcgctgg
      601 tcatgagcgc tgtgcgggct tcggggaaga gggccttcac ctcctcctgg acgcgctcga
      661 cgccggggcc ggagacggcg aggctgtcga cggcgccgca gtcggggcag tggccgggct
      721 ggtgctggcc gtagccgcaa tggtggcagg tgaggcgctg gccgaagcgg tgggcgacga
      781 gccaggccga gcagttgggg cagcgaaagc gaaagccgca ggcgcggcag agggtcaagg
      841 gcgcgtagcc gcggcgattg aggaagagca tggcctgctc gccctgctcg agggcggcgg
      901 cgagagcctg gcgcagcacc ggggcgagga aggcgccctt gggcggcttg tcgcggcgca
      961 gatcgacgag cgccatggcc ggtttggatt gggcggcgaa gcggccctcg acggtgatgt
     1021 gggtccgggc gaggccctgg gtctcgagcg acggggtggc ggagcaggtg acgagccggg
     1081 cgtcctgcag ggcggcgcgc cggcgggcga cgtcgcgggc gtcgtagatg acgccgtcct
     1141 cctgcttgaa gctcgtgtcg tgctcctcgt cgatgacgac gaggccgagt tccggcatgg
     1201 gcaggaagag ggcagagcgc gcaccgacca cgacgggggc ccggccggcg gcggcgagct
     1261 tccaggcgcg gcggcgctgc agcgcggtga ggccggaatg ccagagcagg ggctcggcgc
     1321 cgaagcggct ggtgaagcgg tcgagccact gggcacccaa ggcgatctcg ggcagcagca
     1381 cgagggccga gcggccgcgg gcgagcgtgg cggcgatggc ttccaggtag atctcggtct
     1441 tgccggcgcc gggcaggccc tcgagcagca cctcgccgcc ggagccatcc gcaatggcag
     1501 ccaggagcgc ggccgtggcc tgggcctggg ccggggtcag ccgggccgga tcaggcggct
     1561 ggtagcaggg cgggtcgggc aggccctggg ccaccgtctc gctcctgagc acgcccttgt
     1621 cgaccagcgt cttcaccacc ccggccgaga cgccggccgc ccgggcgagc gctgcggccg
     1681 gctgcgggcc gcccgtggcg aaggccgcca gcacgagctg ctgcttcgcc gagagcggca
     1741 cggcggcacc ggtcggcacc agcacaatgc gggccggctc ggcctcgaag gcggcgggcg
     1801 tgctgaggac gaggcgaagg acggagccca tgggcgtcac cgtggcccgg gcgacggcct
     1861 cgaccagccg gcgaagcggc gccggcaagg gcggctggcc cagcacggcc tggaccggct
     1921 tgagccgcgc ctcggccacc ggccgggcac tgccgcgctc ccagacgacg ccgaccaccc
     1981 ggcgcgggcc gaaggggacg acgacgtagc tgccgagcgg cggcggggtg gtcgcgagct
     2041 cgccctcgag ccggtagtcg aagcaggtgg gcagcggcat cggcagcagg acggaggtgg
     2101 tcgcgagggt catcggatcc gggaggtggt gcggcggttc gcggcgagtg ctagcatcaa
     2161 ggggccgcca cggtaagccg cactggagaa cggtcctgct cgctttcgcc ctgcccgacc
     2221 tcgaggaggc cttcgccgcc tggtgcgact ggctggcgca cgagcggcgg gcggcggcgc
     2281 ggacacaggc cggctaccgc cgcgatctcg ccgccttcgt cgcgttcctc gccgggcatc
     2341 agggtgggcc ggtcgggttg ccggccatcg ccgccctgac cccgcagggc tttcgcggct
     2401 ggctcgcctg gcggctcggc gagggctacg ccaaaagctc gacgcagcgg gcggtggccg
     2461 ccgtgcgcgg ctttctcgat ttcctcgatg cccgccacgg catccacaac ccggcgctgc
     2521 gggcgatgcg cgcccccaaa accaggcgac ccttgccgcg accactggcg gtggcggagg
     2581 tcacggagct gaccgatgcg gcggccaatg ccgaccagcc gccctgggtg ctggcccggg
     2641 acacggcgct gctgctcctg ctgtatggtg cgggcctgcg catcggcgag gccctggagc
     2701 ttgcggccgg cggcctgccg gcggcgggcg agagcacgct tcgggtcacg ggcaagggtc
     2761 gcaaggtccg cctcgtgccg ctcctgccgg tggtggccga ggcgctcgcc gcctatcgcg
     2821 acgcctgccc ccatcccctg ccggcggacg ggccgctctt tcgcggggtg cgtggcgggc
     2881 ccttgcagcc ggccgtggtg cagcggcggg tgcgcgagct gagaagggcg ctgggcctgc
     2941 cggagacggc gacgccgcac agcctgcggc acagcttcgc cacccacctg ctcggtggcg
     3001 gcgccgacct gcgctcgatc caggagcttc tgggccatgc cagcctgagc acgacgcagc
     3061 tttatacggc agtcgacgcc gaggggctgg cccggctcta cacggcggca cacccgcgcg
     3121 cctgagtcgg cccaaaagaa aacggccggg cgtcgccgcc cggccgtccc ccttcaacgg
     3181 cagatcagat cagtcgccga cctggatcag gtcgatatcg acgcgacgat tgagggcacg
     3241 cccctcggcc gtgtcgttgc tggcgatcgg ctgcgactcg ccgaaccagg ccacggtcat
     3301 gcggtcgcgg gcgacaccgg ctgcctcgag gtagtcggcc accgcgttgg cgcggcgctc
     3361 ggacaggccc atgttgtact cttccgtgcc gatgctgtcg gtgtggccgt cgatccggat
     3421 ggtcacgtcc gggttggcca ggagcgtatc ggcggcggca tcgagctcag cggtgaactc
     3481 gggccggacc tcgtacttgt cgaactcgaa gagcacgtcg gacgacaggc tgatgacgtc
     3541 gacctcctcg acgaccggcg cctctagctc ggcgagcccg gcatagaagg catcgcggca
     3601 ggcggcgatg tcgtcgggct ggaaattctc ctcctgctgc tcgatccagc agtcgtagtc
     3661 ggccatggcg cgaccggaaa ccgccggcag gcgctgaccg ggctcgccct gcgcgaccgc
     3721 catgtagcgg gcgcgggcct cgctcagctc ggccgcatgc tcgggcgtga gcacgcgctc
     3781 gctggtcggc tgcggcgcaa cggtctgtcc cccggcgacc gcggatgccc gccgcgcgtg
     3841 gaagatcgtg tcggtgtagt cctgcatgct ttcgtattcg tacttggcat gcccgacata
     3901 gccgtcatac gcagccttct cgaacgcgcc ggtcggcggt gtcgcggacg tcgcggccgc
     3961 ataggtctcg ccggcgtcgc cacgattctt gaacttcgtg ccgagcgttc tgaagttgtc
     4021 gccaccgctg caggaggcca gcacgaacgc tgcgcctgcc gttaccataa ctacacgtga
     4081 gagacgcatt ggccccctcc t
//
LOCUS       DAOTFI010000331         4065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_26130_length_4065_cov_6.770075, whole genome shotgun sequence.
ACCESSION   DAOTFI010000331 DAOTFI010000000
VERSION     DAOTFI010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26130_length_4065_cov_6.770075"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="P5558_22375"
     CDS             <1..589
                     /locus_tag="P5558_22375"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRY27127.1"
                     /translation="EKPFSRDGGLKLLEGNLGRAVIKVSAVKPERRVIEAPAIVFHDQ
                     NELLAAFERGELERDFVAVIRFQGPTANGMPELHKLTPALGVLQDKGFKVALLTDGRM
                     SGASGKVPAAIHLTPEACHGGPIARVKNGDVIRVDSEAGTVEALVDAAEWEAREVVTA
                     DLSHYHEGLGRELFASLRGAVGTADTGASVFEFAA"
     gene            602..1240
                     /gene="eda"
                     /locus_tag="P5558_22380"
     CDS             602..1240
                     /gene="eda"
                     /locus_tag="P5558_22380"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRY27128.1"
                     /translation="MLTTDALMRRAPVIPVIVIDDLATAVPLAKALVAGGLDVLEITL
                     RTAVALDSIRAIIAEVEGAIVGAGTVRDPEQLAAVTKLGCAFAVSPGFTPKLLDAADD
                     SPCPLLPGAATATEMMQLAERGYDRQKFFPAVPAGGLDYLKAVASPLAEIRFCPTGGI
                     DAASAAAFLALGNVLCVGGSWVAPKAAVAAGDWAAITSLAAAAVRSGRHAGS"
     gene            1308..1751
                     /locus_tag="P5558_22385"
     CDS             1308..1751
                     /locus_tag="P5558_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27129.1"
                     /translation="MRAALLMAGWVMLVAPGSIGAASAVEPGPVTLEASELATIGRRA
                     APPPILPAPAPLVIEPPSSPLPGVPRPPTTVLGPSELGAVGRRVQPVPLPLATPAQVA
                     APVTVAAAASPAFARPVPRPVMMAIVQPAAGPVRLDATGLSLIGP"
     gene            complement(1768..2832)
                     /gene="cobW"
                     /locus_tag="P5558_22390"
     CDS             complement(1768..2832)
                     /gene="cobW"
                     /locus_tag="P5558_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HRY27130.1"
                     /translation="MTTPANRQKIPATIVTGFLGAGKTSLIRHLLEESGGRRIALIIN
                     EFGDQGVDRDLIQGCGIPSCREEDMIELANGCICCTVADDFVPTMQKLLDRPERPDHI
                     VIETSGLALPKPLVKAFTWPAVRNEVTVDGVVAVVDAEATAAGLFASDPKAVAAARAA
                     DPGLDHESPLEELFEEQVGCADLVVLNKCDRVDAAGKARAREAVGAHLRPGVHIVEAS
                     HGALDPLVLLGQGAAAEDDLESRHSHHDDGGEHDHDDFESFVVSLPPLARPAEIEARI
                     RAVAGAHEVLRVKGFAVVEGKPMRLAVQAVGARVASYFDRPWAPGEAPLGALVVIGLA
                     GLDRAAISARLAGDTAQAAA"
     gene            complement(2844..3152)
                     /locus_tag="P5558_22395"
     CDS             complement(2844..3152)
                     /locus_tag="P5558_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27131.1"
                     /translation="MPSTDCRTSPPILSLETCDPKGLIRGALAGAPGWSPRELYLTWL
                     MALPGEIDAAHAAALVLDRVAGTASGPAARGLIELLETTTAYPRERLDPGTTQRRRLH
                     "
     regulatory      complement(3190..3418)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3487..>4065
                     /locus_tag="P5558_22400"
     CDS             3487..>4065
                     /locus_tag="P5558_22400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY27132.1"
                     /translation="MPDTRGDVRRGMLMLLVAVTLFPVMDAIAKELATRLPVLQITWG
                     RYAFTVGVILPAILWRHHKALWPVPSPWLQLLRGLAQLAATFLFFLTLTYLPLADTVA
                     LAFLYPLVITLLSPLVLGETVGWRRHSAVAIGFLGALVIIRPGLGFFQPAALFGLGIS
                     LAGAVYLLITRRIAGQAPPLVSLAYASSISLVL"
BASE COUNT          505 a         1426 c         1492 g          642 t
ORIGIN      
        1 ggagaagccg ttcagccggg atggcgggct caagctgctc gagggcaatc tcggccgggc
       61 ggtgatcaag gtctcggcgg tcaagcccga gcgccgcgtg atcgaggcgc cggccatcgt
      121 cttccacgac cagaacgagc ttttggccgc cttcgagcgc ggcgagctcg agcgggactt
      181 cgtcgcggtc atccgcttcc aggggccgac cgccaacggc atgccggagc tgcacaagct
      241 gacgccggcg ctcggcgtgc tgcaggacaa gggcttcaag gtggcgctgc tgaccgacgg
      301 gcggatgtcc ggtgcttcgg gcaaggtgcc ggcggccatt cacctgaccc cggaagcctg
      361 ccatggcggg ccgatcgcgc gggtgaagaa cggcgatgtg atccgggtgg attccgaggc
      421 cggcacggtc gaggcgctgg tcgatgcggc cgaatgggaa gcgcgcgagg tggtgacggc
      481 cgatctttcg cattatcacg aggggctcgg gcgcgagctc ttcgccagcc tgcggggcgc
      541 ggtcggcact gccgacaccg gcgcctcggt ctttgaattc gccgcctgaa ggatgactgc
      601 catgctcacg accgatgccc tgatgcggcg tgccccggtc attccggtga tcgtgatcga
      661 cgacctcgcc acggccgtgc cgctcgccaa agcactggtc gcgggcgggc tcgacgtgct
      721 cgagatcacg ctgcgcacgg cggtggcgct ggattccatc cgggcgatca tcgccgaggt
      781 ggaaggtgcc atcgtcggtg ccgggacggt gcgcgacccg gagcagctcg ccgccgtcac
      841 gaagctcggc tgcgccttcg cggtaagccc cggcttcacg ccgaagctcc tcgatgcggc
      901 cgatgattcg ccctgcccgc tgctgccggg tgcggccacg gcgacggaga tgatgcagct
      961 cgccgagcgc ggctacgacc ggcagaagtt cttcccggcg gtgccggcgg gcgggctcga
     1021 ctatttgaag gcggtggcct cgccgctcgc cgagatccgc ttctgcccga ccggcggcat
     1081 cgacgcggcc agtgctgcgg ccttcctcgc cctcggcaac gtgctctgcg tcggtggctc
     1141 ctgggtggcg ccgaaggcgg ccgtcgccgc cggcgactgg gccgccatca cgagcctcgc
     1201 cgccgcggcc gtgcgtagcg ggcggcacgc ggggagctga ccaggctctt gccgggggct
     1261 tcgggtccgt tcaccatgag cgggcctgaa ccagcgtgga gagtgtcatg cgcgccgcgc
     1321 tgctcatggc cggttgggtc atgctggtcg cgccggggtc gatcggtgcg gccagtgcgg
     1381 tcgagccggg ccctgtgacg ctggaggcga gcgagcttgc gaccatcggc cggcgcgcgg
     1441 cgccgccacc gatattgccg gcgccggcgc cgctcgtcat cgagccgccg agctcgccgc
     1501 tcccgggggt gccccggccg ccgaccaccg tgctcgggcc gagcgagctc ggagccgtcg
     1561 gtcggcgcgt tcagccggtg ccactgccgc tcgccacacc ggcgcaggtg gcagcgccgg
     1621 tgaccgtggc ggccgccgcg tcgcccgctt tcgcccgacc ggtgccgcgt ccggtcatga
     1681 tggccatcgt gcagccggct gccggaccgg tccggctcga tgcgacgggg ctctcgctga
     1741 tcgggccgta ggcgcccgac ggcgcgctca ggcggcggct tgggccgtgt cgccggcgag
     1801 gcgggcgctg atggcggccc ggtccagccc ggcgaggccg atcaccacga gggcgccgag
     1861 cggcgcctcg cccggtgccc aggggcggtc gaaatagctc gccacgcggg cgccgaccgc
     1921 ctgcacggcg agccgcatcg gcttgccctc gaccacggcg aagcccttga cgcgcagcac
     1981 ctcgtgcgcc ccggccacgg cccggatgcg ggcctcgatc tcggccggcc gggcgagcgg
     2041 cggcaggctc acgacgaagc tctcgaaatc gtcgtggtcg tgctcgccgc cgtcgtcgtg
     2101 atgcgaatgg cggctctcga gatcgtcctc ggcagcggca ccctggccga gcaggacgag
     2161 gggatcgagg gcgccgtggc tggcctcgac gatgtggacg cccgggcgga gatgtgcccc
     2221 cacggcttcc cgcgcgcggg ccttgccggc cgcgtccacc cggtcgcact tgttgagcac
     2281 gacgaggtcg gcgcagccca cctgctcctc gaagagctcc tcgagcgggc tttcgtggtc
     2341 gaggccggga tcggccgccc gggccgccgc cacggctttc gggtcgctgg cgaagagccc
     2401 ggccgcggtc gcctccgcgt cgaccacggc gacgacgccg tcgaccgtca cctcgttcct
     2461 gacggcgggc caggtgaagg ccttgacgag cggcttgggc aaagcgagcc ccgaggtctc
     2521 gatgacgatg tggtcggggc gctcgggccg atccaggagc ttctgcatgg tcggcacgaa
     2581 gtcgtcggcg acggtgcagc agatgcagcc attggccagc tcgatcatgt cctcctcgcg
     2641 gcagctcgga atgccgcagc cctggatcag gtcgcgatcg acgccctggt cgccgaactc
     2701 gttgatgatc agggcgatgc gccggccgcc gctctcctcc agcaaatgcc ggatcaggct
     2761 ggtcttgccg gcgccgagga agccggtgac gatggtggct gggatctttt gccggttcgc
     2821 tggcgtcgtc atcgggctct ccatcaatgc aggcggcgac gctgggtcgt gccagggtcg
     2881 aggcgctcgc gcgggtaggc ggtggtggtc tcgagcagct cgatcagccc ccgggcggcg
     2941 gggccggagg ccgtgccggc gacgcggtcg aggaccaggg ccgcggcatg cgccgcgtcg
     3001 atctcgcccg gcagcgccat gagccaggtg agatagagct ccctgggcga ccagccgggg
     3061 gctcccgcga gcgcgccccg gatcagcccc ttggggtcgc aggtctcgag cgacaggatg
     3121 ggtggtgagg ttcggcagtc ggtggacggc atcgggcacg acctttcgcc agggacaccc
     3181 cgccccggat tggaggaatg agctggtggc aggtctcctg gctcgcgggt cggtgccttc
     3241 gaccagcctt cccggaccca tcgggtccag tggcgcgatc gggcgaaggc tcgccgctca
     3301 cagttgcggg ggcagccccg gcctcgatcc tggcgaaagc atgctggatc tcaccgggtt
     3361 cccttttcac cccggtcggc cgaaaggcct agggggaacc accggggcgg actatgacag
     3421 ccgccggccg catccgcaat cgggacggtc ggggccattc tgcaaagggg tgaggacgtg
     3481 ccgccggtgc ccgacaccag gggcgatgtg cgccgcggca tgctgatgct gctcgtggcg
     3541 gtgacgctct ttccggtgat ggacgcgatc gccaaggagc tggcgacgcg cctgccggtg
     3601 ctgcagatca cctggggccg ctacgccttc acggtggggg tcatcctgcc ggccatcctc
     3661 tggcggcacc acaaggcgct ctggcccgtg ccgagcccgt ggctgcagct cttgcgcggg
     3721 ctcgcccagc tcgccgccac cttcctcttc ttcctgacgc tgacctatct gccgctggcc
     3781 gacaccgtgg ccctggcctt cctctatcct ttggtcatca cgctgctctc gcctttggtg
     3841 ctcggcgaga cggtcggctg gcggcgccat tcggcggtcg ccatcggctt tctgggtgcc
     3901 ctcgtcatca ttcggcccgg cctcggcttc ttccagcccg ccgccctttt tggcctcggc
     3961 atcagcctcg ccggcgccgt ctacctcctg atcaccaggc ggatcgccgg tcaggcgccg
     4021 ccgctggtca gcctcgccta cgcctcgtcc atcagcctcg tgctc
//
LOCUS       DAOTFI010000332         4059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_26184_length_4059_cov_6.152098, whole genome shotgun sequence.
ACCESSION   DAOTFI010000332 DAOTFI010000000
VERSION     DAOTFI010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26184_length_4059_cov_6.152098"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..578
                     /locus_tag="P5558_22405"
     CDS             447..578
                     /locus_tag="P5558_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27133.1"
                     /translation="MAEKRLAAKSERERRPFEQAPGFIIVMRGPDHVVEFWRTSSRP"
     gene            858..1667
                     /locus_tag="P5558_22410"
     CDS             858..1667
                     /locus_tag="P5558_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27134.1"
                     /translation="MDDGLFFGHAEVGVFRPGHEALLVGIPRHAATAIGNARLVHDLQ
                     CLNANLEQRVAAEVAERLEAEEMLRQSQKLEALGQLTGGVAHDFDNLLMVISSGINII
                     ERTDDLERRGMVLGRMRESIERGAELTQQLLAVSRKQELKPENIALAELLAGMSVPVV
                     VEEVRRQLPQRHLAWPLWVRPHRARSGPPQGGQLSGVARAVASMDLMARVWSAVSPWL
                     EQRWRLGTGGHGSWPGVPELADNSRSLAFGSGLPHDTKHGLHDATGGLMAG"
     gene            1755..2471
                     /locus_tag="P5558_22415"
     CDS             1755..2471
                     /locus_tag="P5558_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27135.1"
                     /translation="MADASTLAALRARIRSLDGGGARFGARRSSLGDAAIDAHLPWGG
                     LPRAALHEIGGGLASGLAAALASRFLRAAGTLVWCADAALAARQGALYAPGLVRFGID
                     PARLILVRCRRATETLWAAHEALRSPAVACVVAEIEGLDLLAGRRLQLAAEAGGAAGL
                     LLRFGRADTAPSAAVTRWRAVPFLGDDPGARLWLLDLWRVKGGVPASWVVGWDEKTLS
                     FAAVREPADRPAAQRRRAAG"
     gene            2392..3933
                     /locus_tag="P5558_22420"
     CDS             2392..3933
                     /locus_tag="P5558_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HRY27136.1"
                     /translation="MRRRCLSLQFVNLPIDRLRSAAARRGERLPEPLALHAPGARGQV
                     LTAVDVAAAAAGLAPGLRLADARAILPELALAPAAPLADRRLLEGLATACLCYTPSVA
                     LDDDPGGEPDSETAALLLDITGSAHLFGGEQRLLDRIARRLDRLGLGHRLAVADGAMA
                     AWAWARYGDGGVLRGEAAKEALARLPVQALRLGPETTTTLVRLGLRRVGQLRTLPQRA
                     LVRRFGRAPVLALERLMGVTEAPFTPLREPPRHAAGLAWVEPIGRTEDIRAAVLDLLE
                     RLGRELERAVAGARRLELLLFRVDGELARLTVRTGRPSRDTAHLLELFELQLDGLDIG
                     FGIESLRLEVTEAAPLTARQVDLAQDQDVAELARLVDQLQARLGPAAVRRLVPAASHW
                     PEHAQSVADPVGAEPGASDAWLAGQPRPLRLLARPASIEAMAPVPDGPPLLLRRRGRS
                     EPVRCASGPERLEPEWWRTDNPAPRPRDYYRVTDAGGVDLWLYREGRYDEAEPPTWHL
                     HGTFG"
BASE COUNT          526 a         1434 c         1464 g          635 t
ORIGIN      
        1 agcaagcggc tgttaaccgc tgggtcgctg gttcgagtcc ggcccgggga gccaacaccg
       61 gcccctgcca cgcctgtcca tgcgggcccg ccggggtccg cttctcctta aagaagcttg
      121 gcggtaccgc cgcttggtgc gtcttcgggc ggccggggcg tgttgccgca cggcctccgg
      181 caacgcggga gctccaatgg tggggggctc cactgcttct acaacgacgc ctatcggcaa
      241 tcgatcggac ccgcgcgaca ccccgggtcg ctgggtcggc cgggtcacga ggtctgggag
      301 gagatctggg gagattattg gcccgcagat ccgccatgtg atgtccggcg gtggcgccac
      361 gtggcacgtc ggcccgatcc acgacgagag ggcagagaac ggcgtcggcg gcatcctcgt
      421 catctgctcg gaaacgaccc acggtcatgg ccgagaagcg gctggcggcg aagagcgagc
      481 gcgagcgtcg gccgttcgag caggcacccg gcttcatcat cgtcatgagg ggtcccgacc
      541 acgtcgtcga gttctggcga acctcgtcca gaccgtgacc gatgccggcg tcgagctgac
      601 gggcgcccag ttcgacgcgt tcttctacaa cgtcgtcgac gaagccggcg agcactacaa
      661 gctctaccgc tgtccggtgc gccgcgctcg gcattcgaga gatttcccct gccgcgcaac
      721 acgccgattt tcggttcgct cggacgacgt tcctgtcgat cgctctatgg cacgatggac
      781 ccctatcggg aatgccccca ggccacctgc ccgtccgctc ctatctcgcg gtgccggtcg
      841 tgtcgcggac gggggaggtg gatgacggtc tctttttcgg tcacgccgag gtcggggtgt
      901 tccggcccgg gcacgaggcg ttgctggtcg gtattccccg ccacgccgcc acggcgatcg
      961 gcaacgcacg cctcgtgcat gacctgcagt gtctgaacgc caatctcgag cagcgcgtgg
     1021 cagccgaggt cgccgaacgc ctcgaggcgg aggaaatgct ccggcaatcg cagaagctgg
     1081 aggccctggg ccagctcacc ggcggcgttg cccacgattt cgacaacctc ctgatggtga
     1141 tttcctccgg catcaacatc atcgaacgta ctgatgatct cgagcggcgg ggaatggtcc
     1201 tgggccgcat gcgcgaatcg atcgagcgcg gtgccgagct gacgcagcag ttgctcgccg
     1261 tcagtcgcaa gcaggagctg aagcccgaga acatcgccct cgccgagctg ctcgccggca
     1321 tgagcgtgcc ggtggtcgtc gaggaggtgc gccggcaact accgcagcgc catctggcat
     1381 ggccgttgtg ggtccgaccc catcgggcga gatcggggcc gccgcaaggc gggcagttat
     1441 cgggcgtggc gcgggctgtg gcgagcatgg acctgatggc acgagtgtgg tcggcagtgt
     1501 cgccatggct cgagcagcgg tggcggttgg gcacgggtgg gcatggttcg tggccaggtg
     1561 tgccggaact ggcggataac agtcggtctc tggcattcgg cagtggcctg cctcacgaca
     1621 cgaaacatgg cttgcatgat gcgaccggag ggttgatggc gggttaggac caatacggct
     1681 tgcgggcatg gcggcttgac cggggtcatg tccgaaaata gaacatagca agaacatttg
     1741 ccccgggacc gttcatggcc gacgcctcga ccctcgccgc cctgcgggcg cgcatccgca
     1801 gcctcgatgg tggcggggcg aggttcggcg cgcgccggtc gagtctcggc gacgccgcca
     1861 tcgacgcgca tctgccctgg ggcgggctgc cccgggccgc cttgcacgag atcggtggcg
     1921 gcctcgccag tggcctggcg gcggccctgg ccagccgctt cctgcgcgct gccgggaccc
     1981 tcgtctggtg cgcggatgcg gcgctcgccg cccggcaggg cgcgctctac gcccccgggc
     2041 tcgtccgctt cggcatcgac ccggcacggc tgatcctggt ccgctgtcgc cgggcaaccg
     2101 agaccctctg ggcagcgcac gaggcattgc gctcgccggc cgtggcctgc gtcgtggccg
     2161 agatcgaagg gctcgacctg ctcgccggtc ggcgcctgca gctcgccgcc gaggcgggcg
     2221 gtgccgcggg gctgctgctg cgcttcggcc gggccgatac cgccccctcg gccgcggtga
     2281 cccgctggcg ggcggtgccc ttcctcggcg acgatcccgg tgcccgcctc tggctgctcg
     2341 acttgtggcg ggtcaagggg ggtgtgccgg caagctgggt ggtgggatgg gatgagaaga
     2401 cgctgtcttt cgctgcagtt cgtgaacctg ccgatcgacc ggctgcgcag cgccgccgcg
     2461 cggcggggtg agcggctgcc cgagcccttg gcactccatg cgccgggtgc tcgcggccag
     2521 gtcctgaccg cggtcgatgt cgccgctgcc gcggccggcc ttgcccccgg cctgcgcctc
     2581 gccgatgccc gggcgatcct gcccgagctg gcgctggccc cggcagcacc tctggcggat
     2641 cgccgcctgt tggagggcct ggccaccgcc tgcctctgct acaccccctc cgttgccctc
     2701 gacgatgacc ccggcggcga gcctgacagc gagacggcgg cactccttct cgacatcacc
     2761 ggctcggccc atctcttcgg tggcgagcaa aggctgctcg accgcatcgc gcgccggctc
     2821 gaccggctgg gcctcggcca tcgcctggcg gtggccgatg gtgccatggc ggcctgggcc
     2881 tgggcgcgtt acggagatgg gggcgtgctc aggggcgagg cggcgaagga agcgctcgcc
     2941 cgactgccgg tccaggcgct gcgtctcggg ccggaaacca cgaccaccct cgtccggctc
     3001 ggcctgcgcc gggttggcca gcttcgcaca ctgccgcagc gcgccctcgt gcggcgcttc
     3061 ggccgggcgc cggtgctcgc cctcgagcgg ctgatgggcg tgacggaagc acccttcacc
     3121 cccttgcgcg agccgccccg ccacgctgcc gggctcgcct gggtcgagcc gatcggccgg
     3181 accgaggaca tccgggccgc cgtgctcgat ctgctcgaac ggctcggtcg cgagctcgag
     3241 cgggcggtgg cgggcgcgcg ccggctcgag cttctgctct ttcgggtcga tggcgagctg
     3301 gcccggctga ccgttcgcac cggccgcccc agccgcgaca cggcgcatct cctcgagctc
     3361 ttcgagctgc agctcgacgg gctcgacatc ggcttcggca tcgagagcct gcggctcgag
     3421 gtgaccgagg cggcacccct gaccgcacgg caggtcgacc tggcgcagga ccaggacgtc
     3481 gccgagctgg cgcggctggt cgaccagctg caggcgcggc tcggcccggc cgccgtccgc
     3541 cggctggtgc cggcggcgag ccactggccg gagcatgccc agagcgtcgc cgatccggtc
     3601 ggtgccgagc cgggcgccag cgatgcctgg ctcgccggac agccccgtcc cctgcgtctg
     3661 ctcgctcgcc cggcgtcgat cgaggccatg gccccggtgc ccgatggccc gccgctcctg
     3721 ctgcgtcgcc gcggccggag cgagccggtc cgctgcgcca gcggccccga gcgcctcgag
     3781 cccgagtggt ggcgcaccga caaccccgcc cctcggccgc gcgactacta ccgggtgacc
     3841 gatgccgggg gagtggatct ctggctctac cgcgaaggcc gctacgacga ggccgagccg
     3901 ccgacctggc acctgcacgg gacgttcgga tgaggcgagg gcacaggcga ccagcccctt
     3961 cagccgcggc gtcccgccct tgaccgccta tgccgagctg caggtgacca gcaatttctg
     4021 cttcctcagg ggcgcctcgc atccggagga gctggtgga
//
LOCUS       DAOTFI010000333         4044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_26301_length_4044_cov_6.773627, whole genome shotgun sequence.
ACCESSION   DAOTFI010000333 DAOTFI010000000
VERSION     DAOTFI010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26301_length_4044_cov_6.773627"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="P5558_22425"
     CDS             <1..254
                     /locus_tag="P5558_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HRY27137.1"
                     /translation="YGSTEDFAAAFDFGEALDHVIIDVGRAHIWDISSVQALDMVILK
                     FRREGTTVEIIGLNEASASMVDRFAVHDKPAVAATSAGH"
     gene            265..1119
                     /locus_tag="P5558_22430"
     CDS             265..1119
                     /locus_tag="P5558_22430"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY27138.1"
                     /translation="MTRILACIDSSAVYGTSVCDLAAWAAGRLGAGIELLHVVQRKDA
                     VSQRRDLSGAIGLGVKTELLEELTRIDEAEGKLAIARGRLLLDGAEERLKAAGVTDIT
                     KVHRHGGIVETIIEREAAVDFVVLGKRGASSEFATDHIGSKVERVVRSSTKPVLIASR
                     EIRPPQTIVVAFDGSATAIRAVDLVAGSPLFAGLAVHLVAAGTGGQLRSRLDAALALL
                     GERPIAPEAHLVEGQPEQVIAAHMKRLADGLLVMGAYGHSPLRTLIVGSTTTTMIRTV
                     RAPILLMR"
     gene            complement(1152..1565)
                     /locus_tag="P5558_22435"
     CDS             complement(1152..1565)
                     /locus_tag="P5558_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY27139.1"
                     /translation="MHPDQIIEALGMQPHPEGGHYVETYRAPAADGERAVCTAIYFLL
                     RRGERSHWHRVDAAEIWLWHAGSPLALHVDQATVTLGPDLTAGERPQAVVPKDAWQAA
                     ETLGDWTLVSCVVAPGFSFAGFELAPPGWRPEGKK"
     gene            1650..3023
                     /gene="trmFO"
                     /locus_tag="P5558_22440"
     CDS             1650..3023
                     /gene="trmFO"
                     /locus_tag="P5558_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRY27140.1"
                     /translation="MRPVTVVGGGLAGSEAAFQLAEAGVKVRLFEMRPVRGTDAHKTD
                     RLAELVCSNSFRSDDPFGNAVGLLHEEMRRLGSLVMAAGDASKLPAGGALAVDRDAFA
                     ARVQETLEAHPLVEIVREEVRGLPDPADGHAIIATGPLTAPDLAAAIREAGGEDALAF
                     FDAIAPIVHKDSIDLDVAWFQSRYDKTGPGGGTADYINCPLDEAGYQAFIAALLAAEK
                     TSFKEWETSTPYFEGCLPIEVMAERGVDTLRFGPMKPVGLRDPRTGQRPHAVVQLRQD
                     NALGTLYNMVGFQTKLRHGEQTRVFRMIPGLQQAEFARLGGLHRNTFINSPRLLDRQL
                     RLEVAPHIRMAGQITGCEGYVESAAVGLVAGRLTAAEILGIPREPPPAETAHGALVAH
                     ITGGADSATFQPMNVNFGLFPALPEKVRKNERKRAMALRALAGIEGWARPGAAGAALR
                     PAVALAG"
     gene            3151..3861
                     /gene="minC"
                     /locus_tag="P5558_22445"
     CDS             3151..3861
                     /gene="minC"
                     /locus_tag="P5558_22445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRY27141.1"
                     /translation="MSKGVGGLPFQVRGSLQTLLSLKLLRPEDPGFFQALIEKVAHAP
                     DFFRDAPLVLDVAPIQAREPDCLAEIVTWLKEHRVVPVGLQNGSPAWNEAATALGLAI
                     FAAGGSAPPERRPSERAGERAKTRAGAGIVVSQPVRGGQQVVNPDGDLVVTAAVSPGA
                     EVAAAGHIHIYGPLRGRAFAGMNGDERAMIFVDQLDAELLSIAGVHLVNEEIDARLMG
                     KRVRASLVGERLELKPQP"
BASE COUNT          603 a         1443 c         1393 g          605 t
ORIGIN      
        1 tctacggctc gaccgaggat ttcgccgccg ccttcgattt cggcgaggcg ctcgaccatg
       61 tcatcatcga cgtcggccgg gcgcatatct gggacatcag ctcggtgcag gcgctcgaca
      121 tggtgatcct caagttccgc cgcgagggca ccacggtcga gatcatcggc ctgaacgagg
      181 cgagcgccag catggtcgac cgctttgccg tccacgacaa gccggccgtc gcggccacgt
      241 cggcagggca ttgaggaagc acgcatgacc cgcatcctgg cctgcatcga ctcctccgcc
      301 gtctacggga cgagcgtctg cgacctcgcg gcctgggccg ccggccgcct cggcgccggc
      361 atcgagctgc tccacgtcgt ccagcgcaag gacgccgtgt cccagcggcg cgacctgagc
      421 ggtgccatcg gcctcggtgt caagaccgag ctgctcgagg agctgacccg catcgacgag
      481 gccgagggca agctcgcgat cgcccgcggc cggctcctgc tcgatggtgc ggaggagcgc
      541 ctgaaggcag ccggcgtcac cgacatcacg aaggtgcacc gccacggcgg catcgtcgag
      601 accatcatcg agcgcgaggc ggctgtcgat ttcgtcgtgc tcggcaagcg cggagcgtcc
      661 agcgagttcg ccaccgacca tatcggctcc aaggtcgagc gcgtcgtccg gtccagcacg
      721 aagcccgtgc ttatcgcctc gcgcgagatc cgcccgccgc agaccatcgt cgtcgcgttc
      781 gacggcagtg cgacggccat ccgggccgtc gacctggtcg ccggctcacc gctcttcgcc
      841 gggctggccg tgcatctggt cgccgcgggg acaggcggcc agctccggtc ccgcctcgac
      901 gccgccctgg ccctgctggg cgaacggccc atcgcgccgg aggcccatct ggtcgaagga
      961 cagccggagc aggtgatcgc cgcccatatg aagcgtctgg cggacggcct gctggtcatg
     1021 ggcgcctatg gccactcgcc gctccgcacg ctgatcgtcg gcagcacgac gacgacgatg
     1081 atccgcaccg ttcgtgcccc cattctcctc atgcgctagg cacgccggga accgcaataa
     1141 gcggccacat ctcacttttt gccttccggg cgccaacctg gaggggccag ctcgaagccg
     1201 gcgaagctga agccgggcgc gacgacgcag gagaccaggg tccaatcgcc cagggtttcg
     1261 gcggcctgcc aggcatcctt cggcacgacg gcctggggcc gctcgccggc ggtgaggtca
     1321 ggaccgagcg tcaccgtcgc ctggtcgaca tgcagcgcca gcgggctgcc ggcgtgccag
     1381 agccagatct ccgcggcatc cacccggtgc cagtgcgaac gctcgccacg gcgcaggagg
     1441 aagtagatcg ccgtgcatac cgcccgctcg ccgtccgcgg ccggcgcccg gtaggtctcg
     1501 acatagtggc cgccctcggg atgcggctgc atgccgagag cctcgatgat ctgatccggg
     1561 tgcacgggct tgctcctgcg ggcgggcttc ggtatccaac gggactgcag cctatctttg
     1621 ccaagcgcgc aaccatcgca agggctccca tgcgtcccgt gaccgtcgtc ggcggcggcc
     1681 tcgccggctc ggaggccgcc tttcagttgg ccgaggccgg cgtgaaggtg cgtctcttcg
     1741 agatgcgccc ggtgcgcggc accgatgccc acaagaccga ccggctggcc gagctcgtct
     1801 gctcgaactc gttccgttcc gacgacccct tcggcaatgc cgtgggcctg ctgcacgagg
     1861 agatgcggcg cctgggctcg ctggtgatgg cggcgggcga cgcgagcaag ctgccggccg
     1921 gtggggcgct cgccgtcgat cgcgatgcgt tcgcggcccg ggtgcaggag accctggagg
     1981 cgcatcccct ggtcgagatc gtccgggagg aggtgcgcgg cctgcccgac ccggcggacg
     2041 gccacgccat catcgccacc ggccccctca ccgcacccga cctcgccgcg gccatccgcg
     2101 aggccggcgg cgaggacgcg ctcgccttct tcgacgcgat cgcccccatc gtccacaagg
     2161 acagcatcga cctggacgtc gcctggttcc agtcgcgcta cgacaagacc ggcccgggtg
     2221 gcggcaccgc cgactacatc aactgcccgc tcgacgaggc cggctaccag gcgttcatcg
     2281 cggccctctt ggcagccgag aagaccagct tcaaggaatg ggaaacctcc acgccctatt
     2341 tcgagggctg cctgccgatc gaggtgatgg ccgagcgcgg cgtcgacacc ctgcgcttcg
     2401 ggccgatgaa gccggtgggc ctgcgcgatc ccagaaccgg ccagcggccg catgcggtgg
     2461 tccagctccg ccaggacaac gcgctcggca cgctctacaa catggtcggc ttccagacga
     2521 agctgcgcca tggcgagcag acgcgcgtgt tccgcatgat ccccggcctg cagcaggccg
     2581 agttcgcgcg gctcggcggc ctgcaccgca acaccttcat caacagcccg cgcctgctcg
     2641 accggcagct tcggctcgag gtcgccccgc atatccgcat ggcgggccag atcaccggtt
     2701 gcgagggcta tgtcgagagt gccgctgtgg gcctcgtggc cggccgcctg accgcggccg
     2761 agatcctggg catcccgcgc gagccgccgc cggcggagac ggcgcatggt gccctcgtcg
     2821 cccacatcac cggcggtgcc gacagcgcca ccttccagcc gatgaacgtc aatttcgggc
     2881 tcttcccggc gctgcccgag aaggtgcgca agaacgagag gaagcgggcg atggcgcttc
     2941 gcgccttggc gggcatcgag ggctgggcgc gtcccggcgc ggccggcgcg gcgcttcgcc
     3001 cggctgtggc gctggctggc tgagcgattg gccatgccac aacatcttgt gctcggcgtc
     3061 gctctggact acaagctcca gacggattcg gcgctttcca gcatcgctgg ccgatagggg
     3121 aagagggtgg atctaccggt tgccgggaag gtgtcgaagg gtgtcggcgg gctgccgttc
     3181 caggtgcgcg ggtcgctgca gaccctgctt tccctgaagc tgctgcgccc cgaggatccg
     3241 ggcttctttc aggccctgat cgagaaggtg gcgcacgccc cggacttttt ccgcgacgcg
     3301 cccctcgtgc tcgacgtcgc ccccatccag gcgcgggagc cggattgcct tgccgagatc
     3361 gtgacctggc tcaaggagca ccgggtcgtg ccggtcggcc tgcagaacgg ctcgccggcc
     3421 tggaacgagg cggcgacagc gctcggcctc gcgatcttcg cggccggtgg cagcgccccg
     3481 cccgagcggc gcccgagcga gcgggccggc gagcgggcga aaacgcgggc cggtgccggg
     3541 atcgtcgtca gccagccggt gcgcggcggt cagcaggtcg tcaaccccga cggcgatctc
     3601 gtggtcaccg ccgccgtcag tcccggcgcc gaggtcgcgg cggccggtca catccacatc
     3661 tacgggccgc tgcgcggccg tgcgttcgcc ggcatgaacg gggacgagcg ggcgatgatc
     3721 ttcgtcgatc agctcgacgc cgagctcctg tcgatcgccg gcgtccatct cgtcaacgag
     3781 gagatcgatg cccgtctcat gggcaagcgg gttcgtgcct cgctcgtcgg cgagcggctc
     3841 gagctgaagc cccagccctg atacccgaag ctgcccagag ccacccggtc ggaaactgcc
     3901 caaggccggg ggctcgagaa caggaggagt tcgatttgtc gaatgtcatc gttgtcacat
     3961 cgggcaaagg cggggtcggc aagaccacga gcgccgcggc catcggcacc ggtctcgcct
     4021 tgcgcggcaa gaagaccgtc atca
//
LOCUS       DAOTFI010000334         4023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_26476_length_4023_cov_7.353075, whole genome shotgun sequence.
ACCESSION   DAOTFI010000334 DAOTFI010000000
VERSION     DAOTFI010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26476_length_4023_cov_7.353075"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..385
                     /locus_tag="P5558_22450"
     CDS             128..385
                     /locus_tag="P5558_22450"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRY27142.1"
                     /translation="MREVSINEAKTSLSALLQAVARGEEIVITRRNKPVARLVPFAPP
                     PERPTFGMARQALERSGLAREDIDRALAPMTPDELEAWGIG"
     gene            583..771
                     /locus_tag="P5558_22455"
     CDS             583..771
                     /locus_tag="P5558_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27143.1"
                     /translation="MFRHHGFTPLPLDAETAEEAADLAAIHADPFDRALVATARRTSR
                     TSLTKDGTIARYGVPVAW"
     gene            complement(1933..3411)
                     /locus_tag="P5558_22460"
     CDS             complement(1933..3411)
                     /locus_tag="P5558_22460"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY27144.1"
                     /translation="MTRRTQLLFAPSAVLILVGLLGMAWFLVFPPPRPPGPLDRIIFS
                     ADRALLMAAERGGDDTRFLTVLIPYDHSRFFIQNGEPRGFEYELVRAFERELNAKRKR
                     GEPELQAIFVPTRLGDLVPRLASGIGDIAAGGLTITPERAALVDFSRPYFEDVAELVV
                     AHKASPTLERVEDLAGKRVVVLSGSSYVGSLEALNHRFAAEGLAPVEIVEAAPELNTE
                     DLIEMTHAGIIAYTIADQHIAELWQGVLDGIRVDPEIAIAGGGEIAWAIRQDAPESLK
                     EAMNAFLETVRRGTLTGNILFRRYFENRHYVENPLNQTPLDDLVRYRDLFEAEAEAHD
                     FDWRLIAAVAFQESRLNPDARSPRGAVGLMQVLPSTAASVGIDDPAPVDNNIAAGVRY
                     LAHLRDDVFADPNLDDQVREQFMLAAYNAGPTRVRQLRAVTADELGLDPDRWFFNVER
                     AAQARIGNETVRYVTNVNKYWLAYQLGEAILAERQKERGAGN"
     gene            3604..3945
                     /locus_tag="P5558_22465"
     CDS             3604..3945
                     /locus_tag="P5558_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27145.1"
                     /translation="MTEPMNENAVRQAAVRRVMAAGFACLLVAQAGAALAQQAVPEIL
                     RGSSAPPLPPRPATSEPANDSADVVAAGNRVWFVDKDEGTLVGCRLINTSYVGAERIE
                     CSARDLPEASR"
BASE COUNT          625 a         1332 c         1445 g          621 t
ORIGIN      
        1 gccggtggga aggcgtgcct ttccaccttc ctggggccga cggacacgtc ctcatcgcca
       61 tcccgacggt cttgtcctga ccagaattct gatccaggct tctttctgac cagattggag
      121 cctgtcgatg cgcgaggtca gcatcaacga ggcgaagacg tcgctctcgg cgctgctgca
      181 ggcggtcgcc cggggcgagg agatcgtcat caccaggcgg aacaagccgg tggcccggct
      241 cgtgccgttc gcgccgccgc ccgagcggcc caccttcggc atggcgcggc aggcgctgga
      301 gcgctccggc ctcgcccggg aggacatcga tcgggcgctc gcgccgatga cgccggacga
      361 gctggaagcc tgggggatcg gttgaccctc tatctcgccg acgcccgcgc tcatcgactt
      421 ccacacgggc gagccggcgt agccgcggcc gctcgttcgc ctgttcgagg agacgcccga
      481 agccatggcg atcgccgcaa tcacgctttg ggagatcgcc atcgagaccc gtcccggcag
      541 gctccccgat ctccgcggcc ccggttcgcg gccctggccg ccatgttccg ccatcacggc
      601 ttcaccccgc tgccgctcga cgccgagacc gccgaggaag ccgccgacct tgccgccatc
      661 cacgccgacc cgttcgaccg cgccctcgtc gccacggccc gccgcaccag ccgcaccagc
      721 ctcaccaaag acggcaccat cgcccgctac ggcgtcccgg tagcctggtg agcgaggcgg
      781 gcaaccaatt cgattcgaga gcacgagacc cctccagcct cgaaagcctg gtggggccgg
      841 tggggaggcc gtgcctcccc gccatcctcg tccgcaaccg cgccatcacg aatgcttatg
      901 gcacccggcc cggcaccgct gccggcacaa ccggagagcc ccgattcggc cgcccggcgc
      961 ccgggttgtc gcgtattgcg cgacagttgc gcccgggttg tcgcggattc tgcgacatcc
     1021 ggccggcgat tgtcgcggat tccgcgacat aagcattcgt gatgtcgcaa attccgcgac
     1081 atctgccccg aaatgctgcg tttcctgcga cagttggcga cgaaggaacc cgggaaaacg
     1141 acgatggaac ccacggaatc gacgatcgaa cccggcgatt cgacgatcga acccgacttt
     1201 cgaccaacga acccggcgtg ccgcaagctg ttggcatgaa agggaaaggg cttctggaaa
     1261 gcccgatccg ggcagccgcc cgccgccgga atgccgattt gccgagtgaa cccgcggagc
     1321 acttgcgcgg ggtgcggaac taggctataa atcctatatc agacccgtgg cgcgcggcgg
     1381 cgcgggcgag ggtcgccgct cgtggcggag cgtttcggcg ccggccgcgg cggagacggc
     1441 gctgcgcgac cggctgcagc ggctgacagc ctcctgcgcc cggcgcacaa gctcttcgtg
     1501 ccggcgacga cggacagccg gcacggctgg cggatctggc cgaacctggc gcggcaaccg
     1561 gtgccgacgg cgatcggcca actctggatg atcgacacca cccatgtccg cctcgctcgg
     1621 ccgcatccgc cggctcgcca aggaagtcga gaagctgatc gaaaccagca ccgcaatggc
     1681 cgctgtcgcc atcgtccagc cccgcctcgg gcgacttgca ggcaagcggg attgctcagc
     1741 agcttctcgg acaggcgccc agcccggcaa actgccagga atccatgccg gggacgtgca
     1801 agaagcgtgg agattgagct gttctctggt tcggttgcga cgcgtctctt tcgcgaggtc
     1861 gcgcgggttc acgggggcga aagcttgcgg gcctcgtggt gcgaaagctt cgtctttccc
     1921 gcacacgcct cctcagttgc ccgcaccacg ctccttctgc cgctcggcga ggatggcttc
     1981 gccgagctgg taggcgagcc agtacttgtt cacgttggtg acgtagcgga cggtttcgtt
     2041 gccgatgcgg gcctgggcgg cgcgctcgac gttgaagaac cagcgatcgg ggtcgaggcc
     2101 gagctcgtcg gcggtcacgg cgcggagctg gcgcacgcgg gtggggccgg cattgtaggc
     2161 ggcgagcatg aactgctcgc ggacctggtc gtcgaggttg gggtcggcga agacgtcgtc
     2221 gcgcaggtgg gcgaggtagc gcacgccggc ggcgatgttg ttgtcgaccg gggccgggtc
     2281 gtcgatgccg accgaagccg cggtcgaggg caggacctgc atcaggccga cggctcccct
     2341 ggggcttcgg gcatccgggt tcaggcgact ttcctggaag gcgacggcag cgatcaggcg
     2401 ccagtcgaag tcgtgggctt cggcctcggc ctcgaagagg tcgcggtagc ggacgagatc
     2461 gtcgagcggg gtctggttca gcgggttctc gacgtagtgg cggttctcga aatagcggcg
     2521 aaagaggatg ttgccggtca acgtcccgcg gcggacggtc tcgaggaagg cgttcatcgc
     2581 ctccttcagg ctctcggggg cgtcctggcg gatcgcccag gcgatctcgc cgccgccggc
     2641 gatggcgatc tcggggtcga cccggatgcc gtcgagcacg ccctgccaaa gctcggcgat
     2701 gtgctggtcg gcgatggtgt aggcgatgat cccggcatgg gtcatctcga tcaggtcctc
     2761 ggtgttgagc tccggtgccg cctcgacgat ctcgaccggg gcgaggccct cggcggcgaa
     2821 gcggtggttc agggcctcca ggctgccgac ataggagctg ccgctgagca ccacgacgcg
     2881 tttgccggcg aggtcctcga cgcgctcgag cgtcgggctt gccttgtggg ccacgaccag
     2941 ctcggccacg tcctcgaaat agggccggct gaagtcgacg agggcggcgc gctcgggcgt
     3001 gatggtgagg ccgccggcgg cgatgtcgcc gatgccggac gccaggcgcg gcacgaggtc
     3061 gccgaggcgg gtgggcacga agatggcctg cagctcgggc tcgccgcgct tgcgcttcgc
     3121 gttcagctcg cgctcgaagg cgcgcaccag ctcgtactcg aagccccggg gctcgccgtt
     3181 ctggatgaag aagcggctgt ggtcgtaggg gatcagcacg gtgaggaagc gggtatcgtc
     3241 gccgccccgc tcggccgcca tgagcagcgc ccggtcggcg ctgaagatga tccggtcgag
     3301 cgggcccggc ggcctgggag gcggaaagac cagaaaccag gccatgccga gcaggccgac
     3361 caggatcagg acggcggagg gggcgaagag aagctgcgtg cggcgggtca tggatcgggt
     3421 tccccggcgt ggtgatgccg cggcaccttg gttaaggcgg cacgcctact ccgataggtc
     3481 gcaaggtgct tgtacccttc gtccatggtg cggagcatgc ttgaaatcat ggggaaggca
     3541 agtccaggcg cgtggccggt gccgcgcctc gacgttaacg ccaggcgtaa cggaggaaga
     3601 cgcatgaccg agcccatgaa cgagaacgcc gtgcggcagg cggccgtgcg tcgcgtcatg
     3661 gcggcgggtt tcgcctgcct gctcgtggcc caggccggcg cggccctggc ccagcaggcg
     3721 gtacccgaga tcctgcgtgg cagctcggcg ccgccgctgc cgccgcgccc agcgacgagt
     3781 gagccggcga acgacagcgc cgacgtggtg gcggcgggca accgggtgtg gttcgtcgac
     3841 aaggacgagg gcacgctggt cggctgccgg ctgatcaaca cgagctatgt cggcgcggag
     3901 cggatcgaat gctcggctcg cgacctgccc gaggcgtcgc gttgagtcgg ccgaaggctc
     3961 agttccgacc gtagaagtgg tgactgccga gggtcgccgt gcgctgcacc tggtcggcca
     4021 tcc
//
LOCUS       DAOTFI010000335         3993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_26724_length_3993_cov_6.496445, whole genome shotgun sequence.
ACCESSION   DAOTFI010000335 DAOTFI010000000
VERSION     DAOTFI010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26724_length_3993_cov_6.496445"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="P5558_22470"
     CDS             complement(<1..224)
                     /locus_tag="P5558_22470"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001927.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRY27146.1"
                     /translation="MAELLPFDDRDGYIWYNGEMVAWRDAKLHVLSHGLHYGSCVFEG
                     ERAYGGTVFKLTQHSQRLIDSGKILGFDVP"
     gene            425..961
                     /locus_tag="P5558_22475"
     CDS             425..961
                     /locus_tag="P5558_22475"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY27147.1"
                     /translation="MQAQRALNPLFLRDAELERSLALLALLQLDLGSTVGDVLSRAGL
                     ESADLALLARLDSAKHRRQPVTAAELALYFGWTKQRMSRRLQHLAGGGLIERAAVEGD
                     RRKRTLAPTEAGSRLVAEALKLQQRWLRRVFRQAGAADVAGFQNVLQMAAQISPRGAR
                     GLLRAATPATAADDGRDS"
     gene            958..1650
                     /locus_tag="P5558_22480"
     CDS             958..1650
                     /locus_tag="P5558_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY27148.1"
                     /translation="MSEPAHLLVVDDDERLLALLQRFLTRHDHLVSVASDATSARQKL
                     AGLDFDLVVLDVMLPDQSGVELTSELRRTSDVPILLLTARGEAKDRIAGLEAGADDYL
                     VKPFEPRELLLRITRILGRARSARPPAPAPVLRFGPFTFDLGSLELTRGEGEVVHLTS
                     GELALLRTLAERAGAPISRAELGELGNVKGNDRAIDVQILRLRRKLEDDPRQPRHLLT
                     VRGEGYALRTSR"
     gene            1653..3002
                     /locus_tag="P5558_22485"
     CDS             1653..3002
                     /locus_tag="P5558_22485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY27149.1"
                     /translation="MGPIRRLLGRTIVPPSLFWRSILIVVLPLVILQLVLTVIFYNRH
                     WDVVTRWLATGVAGEVALAVELLEAAPTPEAQRELLERMREYLGLRFSLEPGGRLDEA
                     IEENGLDGWRLSHIDQKILESFEQRLDRPFVIDLRSEMPGFVVAFVQLDDGLLRVMAP
                     RQRVTSTTTMLLMYWMVGASLVLIAVAVYFLGLQVRPIRELAKAVENFGRGVDVGDFR
                     PRGPSEIRRAAAAFNVMRDRILRHLSQRTEMLAAISHDLRTPLTRMKLELELLGSDDP
                     MAKELEADVGDMIELVDTYLAFVRGEEGETAEPVELRPLLEQMAQRAQRAGAETELSV
                     PVPITSSLRPTAFRRCLANLVDNAARHGKWVRIAAERRPRAVVITIEDDGPGIPENLR
                     ETVFQPFFRVDTARRRQTGGTGLGLSIARDIVLGHGGELTFESSVKGGAKAMLRLPA"
     gene            complement(3037..3261)
                     /locus_tag="P5558_22490"
     CDS             complement(3037..3261)
                     /locus_tag="P5558_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27150.1"
                     /translation="MSTLSLPQPGEPRQPRPGLGDLFVDTAYVLRVEDEGSVCYGIFD
                     ADGTPLGVAPTRALALAVIRKNGLEPADAH"
     gene            3336..3959
                     /locus_tag="P5558_22495"
     CDS             3336..3959
                     /locus_tag="P5558_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRY27151.1"
                     /translation="MTTPLQAATPPLILASGSAARAAMLRAAGLEFQIAPAPVDEVEL
                     RLALLAEGIASPDAATALAELKAQHVAARQPGDCIVLGADQLLELDGAWLEKPVDRAA
                     AARQLAQLGGRAHRLVSAVVAFRGGARVWHHVASAEITLRALSAGTIEAYLDAAGEAA
                     LRSVGAYELEGLGAQLVTAVRGDHFTVLGLPLLPLLRFLRDQGVLLA"
BASE COUNT          543 a         1407 c         1426 g          617 t
ORIGIN      
        1 tagggcacgt cgaagcccag gatcttgccg ctgtcgatca accgctggct gtgctgggtg
       61 agcttgaaca ccgtgccgcc ataggcccgc tcgccctcga acacgcagga gccgtagtgc
      121 aggccgtggc tcaatacgtg cagcttcgcg tcacgccacg ccaccatctc gccattgtac
      181 cagatgtagc catcgcgatc gtcgaagggg aggagctcgg ccattcgtcg ggctttcgta
      241 tcggccttca ggcttgaaga ggcgcggttt ttcagggatt gcgatcgcta tcacccggtc
      301 ccataagtgt caatccgagg ccggtcgcgg cgcccgcgcc gtttggcatg cctgccatgc
      361 agcggcggtc gccatgtcgg gattgccgga tccggggggg ccgttgctgg ggtcggacgg
      421 aatcatgcag gcgcagcgcg cgctcaaccc gctctttctg cgcgatgccg agctggagcg
      481 cagcctggcg ctgctggcgc tgctgcagct cgatctcggc agcaccgtgg gcgatgtcct
      541 gagccgggcc gggctggaat ccgccgacct cgccttgctg gccaggctgg acagcgccaa
      601 gcaccggcgc cagccggtga ccgcggccga gctcgccctc tatttcggct ggaccaagca
      661 gcgcatgtcg cgtcggctgc agcatctggc cggcggcggg ctgatcgagc gggcggcggt
      721 cgaaggcgac cggcgcaagc ggacgctggc gccgaccgag gccggcagcc ggctcgtggc
      781 cgaggctttg aagctgcagc agcgctggct gcgccgggtc ttccgccaag ccggtgccgc
      841 cgatgtcgca ggcttccaga acgtcctcca gatggcggcc cagatctcgc cgcgcggggc
      901 gcgtggcctg ctgcgggcgg cgacgccggc aacggcggcc gatgacggac gggactcttg
      961 agcgagccgg cgcatctcct cgtggtggac gacgacgagc ggctgctcgc cttgctgcag
     1021 cgcttcctga cccgtcacga ccatctggtg agcgtcgcca gcgacgcgac gtccgcccgg
     1081 cagaagctcg ccgggctcga cttcgatctc gtcgtgctcg acgtgatgct gcccgaccag
     1141 agcggcgtcg agctgaccag cgagctccgg cgcaccagcg acgtgccgat cctgctgctc
     1201 accgcgcgcg gcgaggccaa ggaccggatc gccgggctcg aggccggggc cgacgactac
     1261 ctggtcaagc ccttcgagcc ccgggagctg ctgctccgga tcacccgcat tctcggccgg
     1321 gcccgctcgg cgcggccgcc ggcgccggca ccggtgctgc gcttcggccc cttcaccttc
     1381 gatctcggca gcctcgagct gacccggggc gaaggcgagg tcgtgcatct gacctcgggc
     1441 gagctggcgc tgctgcgcac cttggccgag cgcgccggcg cccccatcag ccgggccgag
     1501 ctcggcgagc tcggcaacgt caagggcaac gatcgggcga tcgacgtgca gatcctccgc
     1561 ctcaggcgca agctggagga cgacccgcgc cagccccggc atctcctgac ggtgcgcggc
     1621 gagggctacg cgctgcgcac gagccgctag caatgggccc gatccgccgc ctgctcgggc
     1681 ggaccatcgt gccgccgtcc ctgttctggc gctcgatcct gatcgtcgtg ctgccgctcg
     1741 tcatcctgca gctcgtgctg acggtgatct tctacaaccg gcactgggac gtggtgacgc
     1801 gctggctggc gaccggtgtc gccggcgagg tcgcgctggc ggtcgagctg ctcgaggcgg
     1861 cgccgacacc agaggcgcag cgcgagctgc tcgagcggat gcgcgagtat ctgggtctcc
     1921 ggttctcgct cgagccgggc gggcggctcg acgaggcgat cgaggagaac gggctggacg
     1981 gctggcggct cagccatatc gaccagaaga tcctcgagag cttcgagcag cggctggacc
     2041 ggcccttcgt catcgacctg cgctcggaga tgccgggctt cgtcgtcgcc ttcgtgcagc
     2101 tcgacgacgg tctcctgcgg gtcatggcgc cgcgccagcg ggtgaccagc acgaccacca
     2161 tgctcttgat gtactggatg gtgggtgcct ccctcgtgct gatcgccgtc gccgtctatt
     2221 tcctcggcct gcaggtgcgg ccgatccgcg agctggcgaa ggcggtcgag aatttcggcc
     2281 ggggcgtgga tgtcggcgat ttccggccgc gcgggccatc cgagattcgc cgggccgccg
     2341 ccgccttcaa cgtcatgcgc gaccgcattc tccgccatct gagccagcgc accgagatgc
     2401 tggcggcgat cagccacgac ctgcggacac ccttgacccg gatgaagctc gagctcgagc
     2461 tgctcggcag cgacgacccg atggccaagg agctcgaggc cgatgtcggc gacatgatcg
     2521 agctggtgga cacctatctc gccttcgtcc gcggcgagga gggcgagacc gccgagcccg
     2581 tcgagctgcg gccgctcctc gagcagatgg cgcaacgggc acaacgcgcg ggggcggaga
     2641 ccgagctttc ggtgcctgtg ccgatcacct cgagcctgcg gccgaccgcc tttcgccgct
     2701 gcctcgccaa tctcgtcgac aatgcggcgc gccatggcaa gtgggtcagg atcgccgccg
     2761 agcgacggcc gcgcgccgtg gtgatcacca tcgaggacga cgggcctggc attccggaga
     2821 acctgcggga gaccgtgttc cagcccttct tccgggtcga cacggcgcgg cgacggcaga
     2881 ccggcggcac cgggctcggc ctcagcatcg cccgcgacat cgtgctcggc catggcggcg
     2941 agctgacctt cgaaagctcg gtcaagggcg gggcgaaggc gatgctgcgg ctgccggcct
     3001 gacccctcga cgccgctccg ccggggcgcc cgccgctcaa tgcgcgtcgg cgggctcgag
     3061 gccgttcttg cggatcaccg cgagggcgag ggcgcgcgtc ggggccacgc cgagcggtgt
     3121 tccgtcggca tcgaaaatgc cgtagcagac actgccctcg tcctcgaccc tgaggacata
     3181 ggcggtatcg acaaagaggt ccccgagccc cggtcggggc tgtcgtggct cacctggctg
     3241 cggcaacgaa agtgtgctca cctctggcct ccgggctcgg tttcgaccgg cggcgagctt
     3301 agggccgtgc aaatgaagga agggttaagc cgcacgtgac gacgcccctg caggcagcga
     3361 ctcccccctt gatcctcgcc tcgggcagcg ccgcgcgggc ggcgatgctg cgcgcggccg
     3421 ggctggagtt ccagattgcc ccggcgcctg tcgacgaggt cgagctcagg ctggcgctcc
     3481 ttgccgaggg tatcgccagc cccgacgccg cgacggcatt ggccgagctg aaggcgcagc
     3541 atgtggcggc ccgccaaccg ggcgactgca tcgtcctcgg cgccgatcag ctgctcgagc
     3601 tggacggggc gtggctcgag aagcccgtcg accgggcggc ggccgcccgc cagctcgcgc
     3661 agctcggggg gcgggcgcac cggctggtca gcgccgtcgt cgcctttcgc ggcggggccc
     3721 gcgtctggca ccacgtcgcc agcgccgaga tcacgctccg cgccctgtcc gccgggacga
     3781 tcgaggccta tctcgacgcc gcgggcgaag cggctctgcg ctcggtcggg gcctacgagc
     3841 tcgaggggct gggggcgcag ctcgtcacgg ccgtgcgcgg cgatcatttc accgtgctcg
     3901 gcctgcccct tttgccgttg ctccggttcc tgcgcgacca gggggtcctg ctcgcgtgag
     3961 cctcggcatc tcccggcgcc cgcacgcccg agc
//
LOCUS       DAOTFI010000336         3965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_26994_length_3965_cov_5.810742, whole genome shotgun sequence.
ACCESSION   DAOTFI010000336 DAOTFI010000000
VERSION     DAOTFI010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26994_length_3965_cov_5.810742"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="P5558_22500"
     CDS             complement(<1..821)
                     /locus_tag="P5558_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY27152.1"
                     /translation="MDRRLLTTIYAWLLLLPAMVLLVAFTHYPTVATFWASFFSTARG
                     RRPASFLGLDHYRRMITDDPVFLQVVWNNFLYALVTIPLSIGIALVMALWVNERLAGR
                     GFLRMAYFTPTVLPMVAAANIWLFFYTPGFGLLNQIIALFGGPDVNFLGSPETALWSV
                     MAVSVWKEAGFFMIFYLAALQSIPPSLKEAAKLEGASDFHYVRRIVLPLLMPTTLFVL
                     VNAVIGAFRVVDHIVAMTGGGPSNASSLLLFYIYETGFKFWDTSYAAALTVVLLAV"
     gene            complement(876..2156)
                     /locus_tag="P5558_22505"
     CDS             complement(876..2156)
                     /locus_tag="P5558_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY27153.1"
                     /translation="MSRKSLLAATAVVALIAAAPSARAELELTLNFPVAVGGPITAII
                     DEYAKTYSEQTPGVTILPVYTGSYQDTITRTVTAFKGGNAPDMAVLLAVDTFTLYDED
                     MIVAISELAQSDEEKAWLASFYPAFLENGSIDGQLWGVPFQRSTPVLYWNKEAFAAAG
                     LDPEVPPATWEEQVELGKQLTLKDSAGNVTQYGVQIPTSGFPYWLFQGLTTQNDVILM
                     NQAGNEVNFDDPRVVEALEYLVDLSTEHGVMAPGIIEWGTTPQDFFQGRTAMMWTTTG
                     NLTNVRNNAPFDFGVAMLPAHVRRGAPTGGGNLFILHSDPEREQASLDFIRWLTTPEN
                     QADWSIKTGYVATSPAAWQTPAMQAYANDFPPATVARDQLEFAVAELSTHENQRVTKV
                     LNDALQAAITGTAEPAAALAAAQEEAMRILAPYR"
     gene            complement(2156..3253)
                     /locus_tag="P5558_22510"
     CDS             complement(2156..3253)
                     /locus_tag="P5558_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY27154.1"
                     /translation="MVLDGLTRRFGETTALAGIDLVILDGAFAVLLGPSGCGKSTTLR
                     LIAGLDTATSGRIRIDGTDVTDRPPAKRGLSMVFQNYALFPHLSVAENILFGLKVRRV
                     PAADRRRRLERAADILGLGALLGRKPSQLSGGQQQRVALARAIVAESRLCLMDEPLSN
                     LDAKLRAEMRHEIRDLQRRLDMTMIYVTHDQAEALSMADQVVLMRDGRIEQDAAPAEL
                     YERPATAFAASFVGTPPMNLLPLDASPHGLVVAGTSGPVLAETPGGHLSDLVVGVRPE
                     ALLPGAGPGFPVTVTDLDYQGADTVLVCDLGGHNLLARVPGHAAAAQGSELQLTCAAT
                     DLAFFSAADGRRVALSAVDPANQPHILGGVV"
     gene            3426..>3965
                     /locus_tag="P5558_22515"
     CDS             3426..>3965
                     /locus_tag="P5558_22515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY27155.1"
                     /translation="MRVVICGGGVIGACTARFLALRGCEVVVVERHRVAGAASGKSGG
                     FLALDWCDGSPLRPLARRSFALHAELAAAFPDRWGYRRLPTAAVTLTRKAGRAAPGLP
                     GWLAPEARFGGWLGTPETTAQVDPEAFTTSMMDLARERGARLVQGVVQGIGRSADGGR
                     ATGVVVDGEVLAADAVVIAM"
BASE COUNT          610 a         1221 c         1557 g          577 t
ORIGIN      
        1 accgcgagca ggacgacggt gagggcggcc gcatagctcg tgtcccagaa cttgaagccg
       61 gtctcgtaga tgtagaagag gaggagcgag ctggcgttgc tggggccccc gccggtcatg
      121 gcgacgatgt ggtcgacgac gcgaaacgcg ccgatcacgg cattgaccag gacgaagagc
      181 gtcgtcggca tgagcagcgg caggacgatg cggcggacat agtggaagtc ggaggcaccc
      241 tcgagcttgg ccgcctcctt caggctgggc gggatcgact gcagcgccgc gaggtagaag
      301 atcatgaaga agcccgcctc cttccagacc gagacggcca tgacgctcca gagcgccgtc
      361 tccggcgagc ccaggaagtt gacgtccggc ccgccgaaga gggcgatgat ctggttcaaa
      421 agcccgaagc ccggcgtgta gaagaagagc cagatattgg ctgccgccac catcggcagg
      481 acggtgggcg tgaagtacgc catgcgcaaa aagccgcgcc cggccagccg ctcgttgacc
      541 cagagcgcca tgacgagggc gatgccgatc gagagcggga tggtgaccag cgcgtagagg
      601 aagttgttcc agaccacctg gaggaagacc gggtcgtcgg tgatcatccg gcgatagtgg
      661 tcgagcccga ggaagctggc ggggcggcga ccgcgcgcgg tcgagaagaa gctggcccag
      721 aaggtcgcga cggtcgggta gtgggtgaag gcgacgagca gcaccatggc cggcagcagg
      781 agcagccagg cgtagatcgt ggtgaggagg cgccggtcca tgcacggatc gccgacgggc
      841 cgggccaccc tggcccggtc ccgccggctg tcggctcagc gatagggggc gaggatgcgc
      901 atcgcctctt cctgcgccgc ggccagcgcc gccgccggct ccgccgtgcc ggtgatcgcc
      961 gcctgcagcg cgtcgttgag gaccttggtg acgcgctggt tctcgtgcgt cgagagctcg
     1021 gccacggcga actcgagctg gtcgcgggcg acggtcgccg gcgggaagtc gttggcgtat
     1081 gcctgcatgg ccggggtttg ccaggcggcc gggctggtcg ccacgtagcc ggtcttgatc
     1141 gaccagtcgg cctggttctc gggcgtggtc agccagcgaa tgaagtcgag ggaagcctgc
     1201 tcgcgctccg ggtcgctgtg caggatgaag agattgccgc cgccggtggg ggcaccacgg
     1261 cggacatgcg ccggcagcat ggcgacgccg aagtcgaagg gcgcgttgtt gcggacattg
     1321 gtgaggttgc cagtggtggt ccacatcatc gccgtccggc cctggaagaa gtcctgcggc
     1381 gtcgtccccc actcgatgat accgggcgcc atcacgccat gctcggtgct gaggtcgacg
     1441 aggtattcca gcgcctccac caccctgggg tcgtcgaagt tcacctcgtt gccggcctgg
     1501 ttcatcagga tgacgtcgtt ctgcgtcgtc aggccctgga agagccagta ggggaagccg
     1561 gaggtcggga tctgcacgcc gtactgggtg acgttgccgg cggagtcctt cagggtgagc
     1621 tgcttgccga gctccacctg ctcctcccag gtggcgggcg ggacctcggg gtcgaggccg
     1681 gcggcggcga aggcctcctt gttccagtag aggaccgggg tcgagcgctg gaagggcacg
     1741 ccccagagct ggccgtcgat cgagccgttc tcgaggaagg ccggatagaa gctcgcgagc
     1801 caggccttct cctcgtcgct ctgggcgagc tcggaaatgg cgacgatcat gtcctcgtcg
     1861 tagagggtga aggtgtcgac cgcgagcagc accgccatgt cgggcgcatt gccgcccttg
     1921 aaggcggtga ccgtgcgggt gatcgtgtcc tggtagctgc cggtgtagac cggcaggatg
     1981 gtcacgcccg gagtctgctc gctgtaggtc ttcgcatact cgtcgatgat ggcggtgatc
     2041 gggccgccga cggcgaccgg gaaattgagc gtcagctcga gctcggccct tgctgacggt
     2101 gcggcggcga tgagcgccac gacggccgtg gcggcgagca atgactttcg ggacatcaga
     2161 caactcctcc caggatgtgt ggctggttcg cggggtcaac ggccgagagc gccacgcggc
     2221 ggccgtcggc ggcggaaaag aaggcgagat cggttgcggc gcaggtgagc tgcagctcgc
     2281 tgccctgggc cgcggcggcg tggccgggga cgcgggcgag caggttgtgg ccgccgaggt
     2341 cgcagacgag aacggtatcg gccccctgat agtcgaggtc ggtcaccgtc acgggaaagc
     2401 ccgggccggc ccctggcaag agggcttcgg ggcggacacc gacgacgaga tcggagaggt
     2461 gcccgcccgg cgtctcggcg aggaccgggc cgctcgtgcc ggcgaccacg aggccatgcg
     2521 gcgaggcgtc gagcggcagg aggttcatcg gcggcgtgcc gacgaagctc gcggcgaagg
     2581 cggtggcggg gcgctcgtag agctcggccg gtgccgcgtc ctgctcgatc cggccgtcgc
     2641 gcatcagcac gacctggtcg gccatgctga gcgcctcggc ctggtcatgg gtgacgtaga
     2701 tcatggtcat gtcgaggcgc cgctgcaggt cccggatctc gtggcgcatc tcggcccgaa
     2761 gcttggcgtc gagattggag agcggctcgt ccatcaggca gagccggctc tcggcgacga
     2821 tggcgcgggc cagcgccacg cgctgctgct ggccgccgga gagctgcgac ggcttgcgac
     2881 cgagaagggc gccgaggccg aggatgtcgg cggcgcgctc cagccgccgt cgccgatcgg
     2941 ccgccggcac gcggcgcacc ttgaggccga agaggatgtt ctcggcgacc gagaggtggg
     3001 ggaatagcgc gtagttctgg aagaccatgg agaggccgcg cttcgccggc ggccggtcgg
     3061 tgacgtcggt gccgtcgatg cggatgcgcc ccgaggtggc cgtgtccagc ccggcgatca
     3121 gccgcagcgt cgtcgacttg ccgcagcccg atggcccgag cagcacggca aaggcgccat
     3181 cgaggatgac gaggtcgatc ccggccaggg ccgtcgtttc gccgaagcga cgggtcaggc
     3241 cgtcgagcac cacttcgggc aagggccacc ttctgcaagc caaccggacg ggaaagccgg
     3301 caggcggcac gctagcggcg tgacagatcg atgacaaccg aggcgacgcc ggccgggttg
     3361 cggacagaca tggccggctg cggcaggggt gggaggcgac cgggcggcgg aacggggagg
     3421 acacgatgcg ggtggtgatc tgcggcggcg gggtgatcgg cgcctgcacg gcccggttcc
     3481 tggcgttgcg tggctgcgag gtggtggtgg tcgagcggca ccgtgtggcc ggcgctgcct
     3541 ccggcaagtc gggcgggttc ctggcgctcg actggtgcga cggctcgccg ctccggccac
     3601 tggcgcggcg cagcttcgcg ctgcatgcgg agctggcggc ggcattcccc gaccgctggg
     3661 gctatcggcg cctgccgacg gcggccgtga ccctgacccg gaaggccggg cgggcggccc
     3721 cgggcctgcc cggctggctg gcgccggagg cgcggttcgg cggctggctc ggcacgccgg
     3781 agacgacggc gcaggtcgat cccgaggcct tcacgaccag catgatggac cttgcccgcg
     3841 agcgcggcgc ccggctggtt cagggtgtcg tccaggggat cgggcggagc gccgacggcg
     3901 ggcgggccac cggagtggtg gtcgatggcg aggtgctggc ggccgatgct gtggtcatcg
     3961 ccatg
//
LOCUS       DAOTFI010000337         3962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_27019_length_3962_cov_7.917584, whole genome shotgun sequence.
ACCESSION   DAOTFI010000337 DAOTFI010000000
VERSION     DAOTFI010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27019_length_3962_cov_7.917584"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..2227
                     /locus_tag="P5558_22520"
     CDS             14..2227
                     /locus_tag="P5558_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase"
                     /protein_id="HRY27156.1"
                     /translation="MLELIGYADKFSVAPGEAIEFKVSAFKGQRFRAEIVRLIHGDAN
                     PEGPGYKHAPVPSPIEGEYQGRNQPIRAGSFGVVPHHPAFAALESFTLMAMVWPTTPA
                     KGRQAILALGDDLALAIGDKGGLVGQIGATTLESEAPLHERHWYLAALTFDATTGTAT
                     LIQRPLLAYPGGADAAMASASATRPDLAGADLLIAAEGSPRRSHLNGKIDSPRLLARA
                     LPPERLEALLVRPLPADLLPDLAFAFDFAQGTRTTRIHDTGPHKLEGQLEHLPARAMK
                     GWCWSGEEMCWWRKPEHYGAIHFHADDLFDAGWATDFAFTVPDDLRSGVYAAHVWCGD
                     AEDGTQEDLIPFFVRPKRGDRDRPKLAFLAPTAAYMAYANDQNHIKGDAAEMVIGRLV
                     TYQPSDIFLYDHPELGLSLYDRHADGSGVCYSSRHRPILNMRPKYSSWLGATGSGLWQ
                     FNADLHITDWLESHMAVEFDVITDEDLHAEGLALLEPYDVVITGTHPEYHSTHMWDAM
                     RAWIDRGGRLMYVAANGWYWRIAFHDELDGVIEVRRAEDGIRTWEADPGEYHHSFTGE
                     YGGLWRRQGRPPQALLGIGFMAQGFDICSYYRRQPDSHDPRAAFVFEGVEDDIIGDFG
                     LIGGGAAGLELDAIRHDLGTPPHTLVLASSEAHSDVIMLVNEEFGVVPPNLKGSEHPN
                     VRADMTFFDTAAGGAVFATGSIAWCGSLSWNGYDNNVSRITGNVLRRFLDPTPFS"
     gene            complement(2220..3713)
                     /locus_tag="P5558_22525"
     CDS             complement(2220..3713)
                     /locus_tag="P5558_22525"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY27157.1"
                     /translation="MSAVDQFAWSTASAIAAQIRARKVSPVEVMEATISRIEARNPSL
                     NAFVFTDFEGARAAARKAEQAVMSGAALGPLHGVPTAMKDLFDFKPGWPTTLGGVRAM
                     KDWRPDLTCPYVERMERAGAILVGKCNSPIMGFRGVCDNYLFGPTGNPFDTSRNPGGS
                     SGGSAAAVADGLVPVAEGTDGGGSIRIPASWSGIFGYKAGFGRVPLNSRPDAFTPAGC
                     FIHEGPLARTVEDAALAMTALAGFDSRDPFSLPDVVDYLGATRRSIQGMRIAWTPNFG
                     VFPVEPVIVETCREALRAFEAAGAVVEEVDFGIERHHLELADLWCKLITPLSVNAFET
                     FKAAGIDLLGDHRDDFPPEFLRWLDYSYRATVPEQLQSQIVRTEINDATQGVLDSHDL
                     ILSPTLAALPVKNGGDGNTLGPAEINGEPVERLIGWCMTYFINFSGHPAASIPAGLAP
                     GNLPVGLQIIGRRHADADVFAASAAFERIRPWFDSYRIPEGRSLTVN"
BASE COUNT          625 a         1435 c         1297 g          605 t
ORIGIN      
        1 aggagctgaa ctgatgctgg agctgatcgg ctatgccgat aaattcagcg tggccccggg
       61 cgaggcgatc gagttcaagg tcagcgcctt caagggccag cgtttccggg ccgagatcgt
      121 ccgcctgatc cacggcgacg ccaaccccga gggtcccggc tacaagcacg cccccgtgcc
      181 atcgccgatc gagggcgagt accagggccg aaaccagccg atccgcgccg gctccttcgg
      241 cgtcgtcccg catcaccccg ccttcgccgc cctcgagagc ttcacgctca tggccatggt
      301 ctggccgacc acgccggcca agggccgcca ggcgatcctg gcgctcggcg acgatctggc
      361 cctggccatc ggcgacaagg gcgggctcgt cggccagatc ggcgcgacca cgctcgagag
      421 cgaggccccg ctccacgagc gccactggta cctggcggcc ctgaccttcg acgccacgac
      481 cggcaccgcc accctgatcc agcgcccact tctggcctat ccgggcggcg ccgacgccgc
      541 catggccagc gcctcggcga cgagacccga cctcgccggc gccgatctgc tgatcgcggc
      601 cgagggcagc ccccgccgaa gccacctgaa cggcaagatc gacagcccgc gcctcctcgc
      661 ccgcgccctg ccgccggagc ggctcgaggc cctcctcgtc cgcccgctgc cggccgatct
      721 cctccccgac ctggctttcg ccttcgactt cgcccaaggc acccgcacga cccgcatcca
      781 cgacacgggc ccgcacaagc tcgagggcca actcgagcac ctgccggcca gggcgatgaa
      841 gggctggtgc tggagcggcg aggagatgtg ctggtggcgc aagcccgagc actatggcgc
      901 catccatttc cacgccgacg acctcttcga cgcgggctgg gccaccgatt tcgccttcac
      961 cgtgccggac gaccttcgtt ccggcgtcta tgcggcgcat gtctggtgcg gcgatgccga
     1021 ggacggcacg caggaggacc tgatcccctt cttcgtccgc ccgaagcgcg gcgaccgcga
     1081 ccggccgaag ctcgccttcc tcgcaccgac cgccgcctac atggcctatg ccaacgacca
     1141 gaatcacatc aagggcgacg cggccgagat ggtcatcggc cggctcgtca cctaccagcc
     1201 gtccgacatc ttcctttacg accaccccga gctcggcctc tcgctctacg accgccacgc
     1261 cgacggcagc ggcgtgtgct actcctcgcg ccaccgcccg atcctcaaca tgcggcccaa
     1321 atacagctcg tggctgggtg cgacgggctc cggcctctgg cagttcaacg ccgacctgca
     1381 catcaccgac tggctcgaga gccacatggc ggtcgagttc gacgtcatca ccgacgagga
     1441 cctgcatgcg gagggcctcg cgctcctcga gccctacgac gtggtgatca ccgggaccca
     1501 ccccgaatac cactcgaccc acatgtggga cgcgatgcgc gcctggatcg atcggggcgg
     1561 ccggctcatg tacgttgccg ccaatggctg gtactggcgc atcgccttcc acgacgagct
     1621 cgacggcgtg atcgaggtgc gccgggcgga ggacggcatc cgcacctggg aggccgatcc
     1681 gggcgagtac caccacagct tcaccggcga atatggcggg ctctggcgcc ggcagggccg
     1741 gccgccgcag gcgctgctcg gcatcggctt catggcccag ggcttcgaca tctgctcgta
     1801 ctacaggcgg cagccggaca gccacgaccc gcgcgccgcc ttcgtcttcg agggcgtcga
     1861 ggacgacatc atcggcgatt tcggcctgat cggcggcggt gctgccgggc tggagctcga
     1921 cgccatccgc cacgacctcg gcacgccgcc gcacaccctg gtcctcgcca gctccgaggc
     1981 gcacagcgac gtcatcatgc tggtcaacga ggagttcggc gtcgtcccgc ccaacctcaa
     2041 gggctccgag caccccaacg tgcgcgccga catgaccttc ttcgacaccg cggccggtgg
     2101 cgccgtcttc gccaccggct cgatcgcgtg gtgcggcagc ctctcctgga acggctacga
     2161 caacaacgtc tcgaggatca cgggcaacgt gctcagacgg ttcctcgatc cgacgccgtt
     2221 cagttgaccg tcagcgagcg gccctccggt atccggtagc tgtcgaacca gggcctgatc
     2281 cgctcgaacg ccgcgctggc ggcgaagacg tccgcgtcgg cgtggcgccg gccgatgatc
     2341 tgcaggccca ccggcaggtt gcccggcgcc agcccggccg ggatcgaggc cgcgggatgg
     2401 ccgctgaagt tgatgaagta ggtcatgcac cagccgatca gccgctcgac cggctcgccg
     2461 ttgatctcgg ccgggcccag cgtgttgccg tcgccgccgt tcttgaccgg caatgccgcc
     2521 aaagtcggcg agaggatcag gtcgtggctg tcgagcacgc cctgggtcgc gtcgttgatc
     2581 tcggtgcgga cgatctggct ctgcagctgc tccggcaccg tggcccggta gctgtagtcc
     2641 agccagcgca ggaactcggg cgggaagtcg tcacggtggt cgcccagaag atcgatgccg
     2701 gccgccttga acgtctcgaa ggcgttgacg ctgagcggcg tgatcagctt gcaccacaag
     2761 tcggctagct cgaggtgatg gcgctcgatg ccgaaatcca cctcctcgac gaccgcaccc
     2821 gcggcctcga aggcgcgcag cgcctcccga caggtctcga cgatcacggg ctcgaccgga
     2881 aagacgccga aattcggggt ccaggcgatg cgcatgccct ggatcgagcg gcgggtggcg
     2941 ccgaggtagt cgaccacgtc cggcaacgag aagggatcgc ggctgtcgaa cccggcgagc
     3001 gccgtcatcg ccaaggccgc gtcctcgacc gtgcgtgcca gggggccctc gtggatgaag
     3061 cagccggccg gggtgaaggc gtcagggcgc gagttgagcg gcacccggcc gaagccggcc
     3121 ttgtagccga agatgccgga ccaggacgcc gggatgcgga tcgacccgcc accgtccgtg
     3181 ccctcggcga cgggcaccag gccatcggcg accgcggcag cgctgccgcc cgaggagccg
     3241 ccggggttgc ggctcgtgtc gaaggggttg cccgtcgggc cgaagagata attgtcgcac
     3301 accccgcgaa agcccatgat cgggctgttg cacttgccga ccaggatggc gccggcccgt
     3361 tccatccgct cgacataggg gcaggtcagg tcgggccgcc agtccttcat cgccctgacc
     3421 ccgccgagcg tcgtcggcca gcccggcttg aagtcgaaga ggtccttcat cgccgtcggc
     3481 acgccgtgca gcggacccag ggcagcgccc gacatgaccg cctgctcggc cttcctcgcc
     3541 gccgcccgcg ccccctcgaa gtcggtgaac acgaacgcgt tcaggctcgg gttgcgcgcc
     3601 tcgatccggg agatggtcgc ctccatcacc tcgactggtg acaccttgcg ggcacgaatc
     3661 tgcgccgcga tcgcactcgc cgtgctccag gcgaactggt ccactgccga catgactggc
     3721 gtccctttca agtcgtgttg atgtgcgggg aaactgccgc aggacgcatg tccgtacaag
     3781 ccggcggtga cgtcagcctg ctggtttcgc ctgcgtctgt tgtctccgct gggaactcat
     3841 ggcggcaggc gtccgttttc ggctgcttgg ccgaggcaca cgcgggtcgg cctatggttg
     3901 gtgcaactgg gtgaagtcct aaatttgctg gaaaatgggt tgggggtaca tcgtcgctga
     3961 gc
//
LOCUS       DAOTFI010000338         3921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_27382_length_3921_cov_7.337558, whole genome shotgun sequence.
ACCESSION   DAOTFI010000338 DAOTFI010000000
VERSION     DAOTFI010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27382_length_3921_cov_7.337558"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="P5558_22530"
     CDS             <1..132
                     /locus_tag="P5558_22530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY27158.1"
                     /translation="NGQYTVWGRVVDGMELVDGLAKGEPPRNPDRMVKVSVLADQPG"
     gene            176..2026
                     /locus_tag="P5558_22535"
     CDS             176..2026
                     /locus_tag="P5558_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRY27159.1"
                     /translation="MLTLTNLTLRIAGRPLLEAADLQIAPGQKVGLVGRNGTGKTSLF
                     RLILGELQPDEGELLLPANWRIGSVAQDAPGGSRTPLEAVLAADLERQALLDERGRSP
                     AAERVADIEMRLHEIGAEAAPAKAARILKGLGFDEAMQGRPLANFSGGWRMRVALAAT
                     LFAEPDLLLLDEPTNHLDLEAALWLEAYLKRYPRTLVLISHDRDLLNSIPDRIVHLEA
                     RQLKAYTGNYDTFDRIRREQRELLEKQKKSLDARRKHLQSFVDRFRYKASKATQAQSR
                     IKMLEKMGTIAPPPPEPDVVFQFPAPDMPPPPLITLDDAAVGYGDTPVLRDLDLRLDP
                     DDRIALLGANGNGKSTLAKLLSGRLEPMLGELHRSRGLRVGFFAQHQTEDLEQDDTAL
                     QHLQRLLPNERAERLRALLGRFGLTQARAETKAQHLSGGEKTRLCLAMVALAKPQLLI
                     LDEPSNHLDIDSREALIEAINEFPGAVVLISHDRHLVELTADRLWLVKDGRVRVYDGD
                     LESYRTELLARAEGEGQRQKGRPARSVGGSERRQLLAPLRKAVKDLEAEIRRLQADRT
                     RIERRLADPATYRGEADIANLESERQGIVAAIARAEERWLEIEGEIEAVG"
     gene            2085..2783
                     /locus_tag="P5558_22540"
     CDS             2085..2783
                     /locus_tag="P5558_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRY27160.1"
                     /translation="MIDLYYWPTPNGHKVTLFLEETGLDYKIHPINIGKGDQFDPAFL
                     KIAPNNRMPAIVDHAPADGGEPVSIFESGAILLYLAEKTGRFISADLRGRVEVLQWLF
                     WQMGGLGPMAGQNHHFGTYAPEKLPYAIKRYVDETNRLYGVLNKRLADRAFVAGSDYS
                     IADMASYPWTVGWERQGQNIDDFPHLKRWQAAIAARPATVRAYELGPKYRSEAPMDDE
                     AKKILFGQTAKVLG"
     gene            complement(3008..>3921)
                     /gene="bmt"
                     /locus_tag="P5558_22545"
     CDS             complement(3008..>3921)
                     /gene="bmt"
                     /locus_tag="P5558_22545"
                     /EC_number="2.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF005718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="betaine--homocysteine S-methyltransferase"
                     /protein_id="HRY27161.1"
                     /translation="SPELWNVDHPDRVTDVHREFVAAGSDLILTNSFGGNAFRLKLHG
                     AEGRVRELNMAAAKAARIAAGEAGRPVLVAGSMGPTGEILAPVGTLDPAEAEAAFRAQ
                     AGALAEGGVDLLWVETMSSAEELAAAVRAASGFGLPVCATMSFDTNGRTMMGLTPAAA
                     LALAHSLDPVPAGFGANCGIGPAQLLATLLGFSRLERRGEVIIAKANCGIPEFRDGAI
                     HYTGTDSIMADYAVLARDAGARIIGGCCGTAGTHLAAMRRALDTVEPGPVPDIARIER
                     LLGPVAAPATEPVATLPRERRRSRPGG"
BASE COUNT          637 a         1396 c         1332 g          556 t
ORIGIN      
        1 aacggccagt acacggtctg gggccgggtg gtcgacggca tggagctggt cgacggcctg
       61 gccaagggcg agccgccgcg caacccggac cgcatggtca aggtcagcgt cctggccgac
      121 cagccgggct gagcccgacg caggcgacag ccttgccgct cgcgccctcg acgccatgct
      181 gactctcacc aacctgacac tccggatcgc cggtcggccg ctgctcgagg cggccgacct
      241 gcagatcgcg cccggtcaga aggtcggtct ggtcggccgc aacggcaccg gcaagaccag
      301 cctcttccgg ctgatcctgg gcgagctgca gccggacgag ggcgagctgc tgctgccggc
      361 caactggcgc atcggcagcg tcgcccagga tgcgcccggc ggctccagga caccgctcga
      421 ggcggtgctc gccgccgacc tcgagcgcca ggcgctgctc gacgagcgcg gccgctcgcc
      481 cgcggccgag cgcgtcgccg atatcgagat gcggctccac gagatcggtg ccgaggcggc
      541 gccggccaag gccgcccgca tcctcaaggg cctgggcttc gacgaggcga tgcagggccg
      601 gccgctcgcc aacttctcgg gcggctggcg gatgcgcgtg gccctcgcgg cgaccctctt
      661 cgccgagccg gatctcctgc tgctcgacga gccgaccaac catctcgacc tcgaggccgc
      721 cctctggctc gaggcctatc tcaagcgcta cccgcgcacg ctcgtcctga tcagccacga
      781 ccgcgacctc ttgaacagca ttcccgaccg gatcgtccat ctcgaggccc ggcagctcaa
      841 ggcctatacc ggcaattacg acacgttcga ccggatccgc cgcgagcagc gcgagctttt
      901 ggagaagcag aagaagagcc tggacgcgcg ccgcaagcac ctgcagtcct tcgtcgaccg
      961 cttccgctac aaggcgtcga aggccaccca ggcgcagagc cggatcaaga tgctggagaa
     1021 gatgggcacc atcgctccgc cgccgcccga gcccgacgtc gtcttccagt tcccggcacc
     1081 ggacatgccg ccgccgccct tgatcacgct cgacgatgcg gcggtcggct acggcgacac
     1141 cccggtgctc agggacctcg atctccggct cgatcccgat gatcgcatcg cgcttctggg
     1201 tgccaacggc aacggcaagt cgacgctcgc caagctcctc tcgggccggc tcgagccgat
     1261 gctgggcgag ttgcaccgca gccgcgggct gcgcgtcggc ttcttcgccc agcaccagac
     1321 cgaggatctc gagcaggacg acacggccct gcagcacctg cagcgcctcc tgccgaacga
     1381 gcgggccgag cgcctgcgcg cgctcctcgg ccgcttcggc ctgacccagg cgcgggccga
     1441 gaccaaggcg cagcatctct cgggcggcga gaagacgcgg ctctgcctcg ccatggtggc
     1501 gctcgccaag ccgcagcttt tgatcctcga cgagccctcg aaccacctcg acatcgacag
     1561 ccgcgaggcg ctgatcgagg cgatcaacga attcccgggt gccgtggtgc tgatcagcca
     1621 cgaccggcat ttggtggagc tcaccgccga ccgcctctgg ctggtcaagg acggccgggt
     1681 gcgggtctat gacggcgatc tcgagagcta tcgcaccgag cttctggcgc gcgccgaggg
     1741 cgagggccag cggcagaagg gccggccggc ccgttcggtg ggcggcagcg agcgacgcca
     1801 gctgttggct cccctgcgca aggcggtcaa ggacctggag gccgagatca ggcgactcca
     1861 ggccgacagg acccggatcg agcggcgcct cgccgatccc gcgacctacc ggggcgaggc
     1921 cgacatagcc aacctcgaga gcgagcggca gggtatcgtc gccgccatcg cccgggccga
     1981 ggaacgctgg ctcgagatcg agggtgaaat cgaggcggtc ggttgacggc tgcggcgccg
     2041 accgccatct cgccgttcag gctccaagga aaggcaggca agccgtgatc gatctctatt
     2101 actggccgac ccccaatggg cacaaggtca ccctcttcct cgaggagacc gggctcgact
     2161 acaagattca tccgatcaat atcggcaagg gcgaccagtt cgatccggcc tttttgaaga
     2221 tcgccccgaa caaccggatg ccggcgatcg tcgaccatgc gccggccgac ggcggcgagc
     2281 ccgtctcgat cttcgaatcc ggtgcgatcc tgctctatct ggccgagaag accggccggt
     2341 tcatcagcgc cgacctgcgc ggccgcgtcg aggtgctgca gtggctcttc tggcagatgg
     2401 gtgggctcgg gccgatggcc gggcagaacc atcatttcgg cacctacgcg ccggagaagc
     2461 tgccctacgc gatcaagcgc tatgtcgacg agaccaaccg gctctatggc gtgctgaaca
     2521 agcggctggc tgatcgcgcc ttcgtcgccg gctccgacta ctcgatcgcc gacatggcga
     2581 gctatccctg gaccgttggc tgggagcggc agggccagaa catcgacgac ttcccgcacc
     2641 tgaagcgctg gcaggcggcg atcgccgccc ggccggcgac cgtccgagcc tatgagctcg
     2701 ggcccaaata tcggagcgag gcgccgatgg acgacgaggc caagaagatc cttttcggcc
     2761 agaccgccaa ggtcctgggc tgagagcctg cccgagacgt tttcgtgatg tttcatcggc
     2821 ttacgggcaa gctcggcgac gaagccggag ccagccgtaa gccgaccgga ggtgatcttg
     2881 gcagcggcac ctgcgtcgtc gttgatcgcc cgacgatgcg cagcatcgac gggcgatcaa
     2941 ctcctcgcat ctgctcactg ccaaactcat gaaacaccac gaaaacgtct cgggtaggct
     3001 ccgagcctca gcctccgggt cgggagcgcc gccgctcgcg cggtagcgtc gccaccggct
     3061 cggtcgcggg tgcggcgacc gggccgagga gacgctcgat ccgggcgatg tccggcaccg
     3121 gccccggctc gaccgtgtcg agtgcccggc gcatggcggc gaggtgcgtg ccggccgtgc
     3181 cgcagcagcc gccgatgatg cgcgccccgg catcgcgcgc caggacggcg tagtcggcca
     3241 tgatggaatc ggtgccggtg tagtggattg ccccgtcgcg gaattcggga atgccgcaat
     3301 tggccttcgc gatgatcacc tcgccgcgtc gctcgagccg gctgaagccg aggagcgtgg
     3361 ccaggagctg cgccgggccg atgccgcaat tggcgccgaa gccggcgggc acggggtcga
     3421 gcgaatgggc cagcgccagg gccgcggcgg gcgtcaggcc catcatcgtc cggccattgg
     3481 tgtcgaagct catggtggcg cagaccggca ggccgaagcc gctggcggcc cggacggcgg
     3541 cggccagctc ctcggccgac gacatggtct cgacccagag gagatcgacc ccgccctcgg
     3601 cgagggcccc cgcctgggcc ctgaaggccg cctcggcctc ggccgggtcg agcgtgccga
     3661 ccggggccag gatctcgccg gtcggaccca tcgagccggc aaccagcacc ggtcgcccgg
     3721 cctccccggc ggcaatgcgt gccgctttcg cggccgccat gttcagctcg cgcacacgac
     3781 cctccgcccc gtgcagcttc agccggaagg cattgccgcc gaagctgttg gtcaggatca
     3841 ggtcggagcc ggccgccacg aactcgcgat gcacgtcggt cacccgatcc ggatggtcga
     3901 cattccagag ctccgggctg t
//
LOCUS       DAOTFI010000339         3920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_27391_length_3920_cov_5.788875, whole genome shotgun sequence.
ACCESSION   DAOTFI010000339 DAOTFI010000000
VERSION     DAOTFI010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27391_length_3920_cov_5.788875"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..409)
                     /locus_tag="P5558_22550"
     CDS             complement(74..409)
                     /locus_tag="P5558_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27162.1"
                     /translation="MVYAIPAGLLLAATALGTTWVSRQFDAAPPAAWTARRHMREIVS
                     VVVMTAWMVAFLVAGAAFQHLQTGLESLGEFAWGWAIAGPGALPAIVLFVRGRRIARA
                     SRPARLSRR"
     gene            complement(564..1331)
                     /locus_tag="P5558_22555"
     CDS             complement(564..1331)
                     /locus_tag="P5558_22555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY27163.1"
                     /translation="MTVFATYPSLRDRSVLVTGGGSGIGAAIVHAMAGQGSKVAFIDF
                     DAEASRKTVDEVVAAGHAAPLFLEADLRDIEALRAAVAKAAEAHGDITVLVNNAARDD
                     RHPFAEVTSEYWDDRIAMNLKHQFFCAQAVQPMMKRQGGGSIVNMGSVSWVAGQGGMH
                     AYTAAKAGVLGLTRGQARDFGPDNIRVNCIAPGWIMTERQQRLWLDAAGEAELMQRQC
                     LKRKLQPDEIARVVLFLAADDSSAMTNQQYLADGGWV"
     gene            complement(1339..2865)
                     /locus_tag="P5558_22560"
     CDS             complement(1339..2865)
                     /locus_tag="P5558_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY27164.1"
                     /translation="MSHTLLAAFALVFDPYVIGVMVAAGAFGLFVGAMPGLTATLATA
                     LLVPITFFMEPIPALAAIVSTTAMAIFAGDLPGALLRIPGTPASAAYVDDTYKLTQAG
                     KANLGLGTSLVCAVLGGLVGATILAFTAPLLAEVALSFSTFEYFWLATFGLTCAAFVS
                     TGSPVKGLVSLLLGLFIATIGIDIMAGQPRFTFGSVELMGGVSFIPAMIGMFAVSEVL
                     RWALGTTGGRDQMAKVSQASGAILRPGLASLRRYKLGAARGSVIGTLIGILPGAGADI
                     AAWVTYAISKKASKEPEKFGTGHVEGLVDAGTANNASLGGAWVPALVFGIPGDSITAI
                     VIGILYMKGMNPGPMVMLNQPELLYAVFLAFFVANLLLLPLGWLAIKSYGQMLKVPRE
                     VLMPVILLLCMVGAFAINNTVFGVTVMLVLGVVAFVMEENGFPVAPAILGLVLGSMVE
                     RNFMQSMIKADGNLMAFFERPIAAVLGVVTLVIWLAVVVSAVRGRRRVPPVLATTPNE
                     "
     gene            complement(2896..3369)
                     /locus_tag="P5558_22565"
     CDS             complement(2896..3369)
                     /locus_tag="P5558_22565"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY27165.1"
                     /translation="MRFNDAILGLVILLGGLAIVLRASTFPATHGQAYGPDFFPSIIG
                     TGLILSGLGLIVAGWRARAVTGWVDVAGHRLDRLVDAGLVLLAILAFILFLDRLGFVL
                     TAGIGMYVLLVRFRGGHWLASLLITVALVLVVDWAFRAMLLVPLPQGALLPRLPW"
     gene            complement(3406..>3920)
                     /locus_tag="P5558_22570"
     CDS             complement(3406..>3920)
                     /locus_tag="P5558_22570"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRY27166.1"
                     /translation="ASGTGQGGIWHLALAGMLQTAGMEPDRVPWVPSEGAAPGLQDLV
                     AGGVDVVTCSIPEAQSLIDAGRVKSLAIMASERAANFPDVPTLEEKGIDWKLGAWRGV
                     AGPKGLPDEVVSTIVPLLEKIHASDEFRSFMTDRGYGMVFQGPEGFEAFMAQSNESLG
                     SVMKELGMAK"
BASE COUNT          658 a         1318 c         1431 g          513 t
ORIGIN      
        1 gagcgctggc tcttcttcgc cgaggccgag catctggtga tgctctacta cggcagggag
       61 tcggtctagg ccgctaccgc cgggagaggc gcgcgggccg tgacgctcgc gcgatgcggc
      121 gcccccgcac gaagaggacg atggccggca gggcgccggg gccggcgatg gcccagcccc
      181 aggcgaactc gccgagcgat tccaggcccg tctgcaagtg ctggaaggcg gcacccgcca
      241 cgaggaaggc gaccatccag gccgtcatga cgacgacgga cacgatctcc cgcatgtggc
      301 gacgggccgt ccaggcggcc ggtggcgccg cgtcgaactg gcgggagacc caggtggtgc
      361 cgagtgccgt ggcggcgagc aacaggcccg ccggaattgc gtagaccatg acgagcgacc
      421 cactccgatc agccccgcat cggccggccg gccggacgcc ccggtcgcac gtgcctgcgg
      481 acgcgctcag atagtcgccc ggcgcccgtt gcgctcgtgc atttgcgccg ggggtcccgg
      541 acgagacagc gcaaagctgc aggtcagacc cagccgccgt ccgccagata ttgctggttg
      601 gtcatggcgc tgctgtcgtc ggcggcgagg aagaggacga cgcgggcgat ctcgtccggc
      661 tgcagcttgc gcttgaggca ctggcgctgc atcagctcgg cctcgcccgc cgcgtcgagc
      721 cagaggcgct gctgccgctc ggtcatgatc cagcccgggg cgatgcagtt gacgcggatg
      781 ttgtccgggc cgaaatcgcg cgcctggccg cgggtcaggc cgagcacgcc cgccttcgcc
      841 gccgtgtagg cgtgcatgcc gccctggccc gccacccagc tcaccgagcc catgttgacg
      901 atcgacccgc cgccctgccg cttcatcatc ggctggaccg cctgggcgca gaagaactgg
      961 tgcttgaggt tcatggcgat ccggtcgtcc cagtactcgg acgtcacctc ggcgaaggga
     1021 tggcggtcgt cgcgcgccgc gttgttgacc agcacggtga tgtcgccatg cgcctcggcc
     1081 gccttcgcga cggcggcgcg cagggcctcg atgtcgcgca ggtcggcctc gaggaagagc
     1141 ggtgcggcgt ggccggccgc caccacctcg tcgaccgtct tgcggctcgc ctcggcatcg
     1201 aaatcgatga aggcgacctt gctgccctgc ccggccatgg cgtggacgat ggcagcaccg
     1261 atccccgagc cgccgccggt gaccaggacg gagcggtccc tcaggctcgg gtaggtggcg
     1321 aagacggtca tcgtttcctc attcgttggg ggtggtggcg agaaccggcg gcacccggcg
     1381 tcggccccgc acggcgctga cgacgacggc gagccagatg accagggtga cgacgccgag
     1441 cacggcggcg atcggccgct cgaagaaggc catgaggttg ccgtcggcct tgatcatcga
     1501 ctgcatgaag ttgcgctcga ccatgctgcc gaggacgagc cccaggatcg ccggcgccac
     1561 gggaaagccg ttctcctcca tgacgaaggc gaccacgccg agcaccagca tgacggtcac
     1621 gccgaagacc gtgttgttga tggcgaacgc acccaccatg cagagcagca ggatgaccgg
     1681 catcaggacc tcgcgcggca ccttgagcat ctgtccgtag gacttgatgg cgagccagcc
     1741 gagcggcagc aggaggaggt tggcgacgaa gaaggcgagg aagacggcat agagcagctc
     1801 gggctggttg agcatcacca tcggccccgg gttcatcccc ttcatgtaga ggatgccgat
     1861 gacgatggcg gtgatcgaat cgcccgggat gccgaagacg agggccggga cccaggcccc
     1921 gccgaggctc gcattgttgg cggtgccggc gtcgaccagc ccctcgacat ggccggtgcc
     1981 gaacttctcc ggctccttcg aggccttctt cgagatcgcg taggtgaccc aggcggcgat
     2041 gtcggcgccg gcacccggca ggatgccgat cagcgtgccg atcacgctgc cacgcgcggc
     2101 accgagcttg tagcggcgca gcgacgcgag gcccggccgg aggatggcgc cgctcgcctg
     2161 gctcaccttg gccatctggt cgcgcccgcc ggtggtgccg agcgcccagc gcagcacctc
     2221 ggagacggcg aacatgccga tcatcgccgg aatgaagctg acgccgccca tcagctcgac
     2281 cgagccgaag gtgaagcgcg gctggccggc catgatgtcg atgccgatgg tggcgatgaa
     2341 gaggccgagc agcagcgaga ccagcccctt caccgggctg ccggtcgaga cgaaggcagc
     2401 gcaggtgagg ccgaaggtgg cgagccagaa atactcgaag gtcgagaagc tgagcgccac
     2461 ctcggcgagc agcggtgccg tgaaggcgag gatggtggca cccacgagcc cgcccaggac
     2521 ggcgcagacg agcgacgtgc cgagcccgag attggccttg cccgcctggg tcagcttgta
     2581 ggtgtcgtcg acataggcgg ccgaggccgg tgtccccggg atgcgcagga gggcacccgg
     2641 caggtcgccc gcgaagatgg ccatggcggt ggtcgagacg atggcggcaa gtgcggggat
     2701 cggctccatg aagaaggtga tgggcacgag cagcgcggtc gccagggtcg ccgtcagccc
     2761 gggcatggcg ccgacgaaga gcccgaaggc gccggccgcc accatcaccc cgatcacata
     2821 gggatcgaag accagggcga aggcggcaag gagcgtgtgg ctcatgggcg agaaccggtc
     2881 accatggcag gcgcctcacc agggcagccg gggcaggagg gcgccctgcg gcagcggcac
     2941 cagcagcatc gcccgaaagg cccagtccac cacgaggacg agggcgacgg tgatcaggag
     3001 cgaggcgagc cagtggccgc cgcgaaagcg gacgagcagc acgtacatgc cgatgcccgc
     3061 ggtcaggacg aagccgagcc ggtcgaggaa gaggatgaag gcgaggatcg ccaggagcac
     3121 gaggccggcg tcgaccagcc ggtcgagccg gtggccggcg acgtcgaccc agccggtgac
     3181 ggcccgggcg cgccagccgg caacgatcag gccgagcccg gagaggatca ggcccgtgcc
     3241 gatgatcgag gggaagaagt ccgggccgta ggcctggccg tgcgtcgcgg ggaaggtgct
     3301 ggcccgcagc acgatggcga ggccgcccag caggatcacg aggccgagga tggcgtcgtt
     3361 gaacctcatc ggtgcggccc cgcccgagct ttgggcccgg cccggctact tcgccatgcc
     3421 gagctctttc atcacgctgc cgagcgactc gttgctctgg gccatgaacg cctcgaagcc
     3481 ctcggggccc tggaacacca tgccgtagcc ccgatcggtc atgaagctgc ggaactcgtc
     3541 gctggcatgg atcttctcga gcagcggcac gatggtgctc accacctcgt ccggcaggcc
     3601 cttcggcccc gccacgccgc gccaggcgcc gagcttccag tcgatgccct tctcctcgag
     3661 ggtcggcacg tcggggaagt tggcagcccg ctcgctcgcc atgatggcga ggctcttcac
     3721 ccggccggca tcgatcagcg actgggcctc gggaatcgag caggtgacga catcgacgcc
     3781 gccggcgacg aggtcctgca ggccgggggc ggcaccctcg gacgggaccc agggcacccg
     3841 gtccggctcc atgccggcgg tctgcagcat gccggcgagc gccaggtgcc agatgccgcc
     3901 ctggccggtg cccgacgcct
//
LOCUS       DAOTFI010000340         3871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_27839_length_3871_cov_7.434224, whole genome shotgun sequence.
ACCESSION   DAOTFI010000340 DAOTFI010000000
VERSION     DAOTFI010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27839_length_3871_cov_7.434224"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="P5558_22575"
     CDS             <1..558
                     /locus_tag="P5558_22575"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRY27167.1"
                     /translation="SLALGTSEVTPLELTAAYQPFALEGVRRPPFGVERVTDAAAGEL
                     YAHRAVEAQVVAPETARQMRNMLGNVVRRGTGRAAALPDRQGFGKTGTTQDNRDAWFV
                     GFAGAYTLGVWVGRDDNRPMPGVSGADLPARIWREVMVETAPGEVLAGLALPQAKPAP
                     ARREGDSYVLHTVKSWFDRVLGATN"
     gene            complement(566..823)
                     /locus_tag="P5558_22580"
     CDS             complement(566..823)
                     /locus_tag="P5558_22580"
                     /inference="COORDINATES: protein motif:HMM:NF039461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HRY27168.1"
                     /translation="MALPRITTATVIKLVVASLAVGLALAWLGKDPIDLLYWVRDGIT
                     DVLGDASGWVQWALKYIFIGAVVVVPIWLVHYLWRAMRGKS"
     gene            981..2009
                     /locus_tag="P5558_22585"
     CDS             981..2009
                     /locus_tag="P5558_22585"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRY27169.1"
                     /translation="MTRPEEALHLVAVHAEGEIGKVIVAGAPTIPGRSVLDKLVHLNT
                     VDDGLRRFCILEPRGGPQASAILLLDPIEPGTDAGFIVMQPDTCHAMSGSNAICVTTA
                     LLETGRVAMREPETRLVLDTAAGPVPVLATCRAGKCERVQLDMPWSFVVEDGLSFEVE
                     GQGRVEAAIAFGGVFYLLVEAAPLGLDIAPGSARRLVEAGMAILEEAQRRWQPTHPER
                     PGIEGIAYLMFMAESVGRRTNATIMPPGRVDRSPCGTGSSARLALLHARGRANGARAT
                     TFHSIIGSQFEAGISATGEVAGRPAIRPWISGRGWIYAEEKLLVDRRDPFLAGHALAD
                     VYGPGLDR"
     gene            2006..3169
                     /gene="hemW"
                     /locus_tag="P5558_22590"
     CDS             2006..3169
                     /gene="hemW"
                     /locus_tag="P5558_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRY27170.1"
                     /translation="MSDDRTIALYVHWPFCRVKCPYCDFNSHVRAEVDQGRWRRALLA
                     ELDHFAVEVGPRRLTSIFFGGGTPSLMPPATTAAVIDRAIQHFVADPALEITLEANPT
                     SVEAGRFADYRTAGVNRASIGVQALDDRALKALGREHDAAEAIAAIELAGRTFPRWSF
                     DLIYARPGQTVAAWRAELGRVLDLAGEHLSLYQLTIEPGTVFHARQRAGLLVTPRDDR
                     QAALFELTQELTDAAGFGAYEVSNHARPGGECRHNLTYWRYEPYIGIGPGAFGRLPAG
                     AGRVGTATLRAPEAWLRAVEGRGHGELQREVLAPEDLVVEALITGLRLTEGVPEARLA
                     ALDPDWRTRLDPRARVRLQEAEHLDSEAGALRLTGAARLCLDAVLRELVAVPA"
     gene            complement(3233..>3871)
                     /locus_tag="P5558_22595"
     CDS             complement(3233..>3871)
                     /locus_tag="P5558_22595"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY27171.1"
                     /translation="ENKDLRIAYVGTKNYDIGVNLAKRVMELKPDGGTICIQSGGAAA
                     ANHNERMQGIRDTLSGEASSEPPGNRLEGANGWTEIDGCPLYTDDDFMRANQQLEDIL
                     AREPDLDAFVPTGGFPQFVPDAYRAAVTPHKGRLESGELALVVADTLPVQMDLMAEGL
                     SLGQVGQRPFEMGYQSMYFLRDIVKDGKNPEDPTYTGLDVCSPENADTCIGG"
BASE COUNT          551 a         1294 c         1431 g          595 t
ORIGIN      
        1 tcgctggcgc tcggcacttc cgaggtgacc ccgctggagc tgaccgcggc ctatcagcca
       61 ttcgccctgg agggtgtgcg tcggccgccc ttcggggtcg agcgggtgac cgatgcggcg
      121 gcgggcgagc tctacgcgca tcgggccgtc gaggcccagg tcgtggcacc ggagaccgcg
      181 cggcagatgc gcaacatgct gggcaatgtc gtgcgtcgcg gcaccggccg ggcggcggcg
      241 ctgccggacc ggcagggctt cggcaagacc ggcacgacgc aggacaaccg ggatgcctgg
      301 ttcgtcggct tcgccggcgc ctacaccctg ggtgtctggg tcggccggga cgacaaccgg
      361 cccatgccgg gtgtctcggg cgccgacctg ccggcgcgga tctggcgcga ggtgatggtc
      421 gagacggcgc cgggcgaggt gctggccggc ctggcgctgc cgcaggcgaa gcccgcgccg
      481 gcgcggcggg agggcgacag ctacgtgctg cacacggtga agagctggtt cgaccgggtg
      541 ctgggcgcca cgaactgaac ggggctcagg acttgccgcg catcgcccgc cagagatagt
      601 ggacgagcca gatcggcacc acgacgacgg cgccgatgaa gatgtacttg agcgcccact
      661 gcacccagcc gctggcgtcg cccaggacgt cggtgatgcc gtcgcggacc cagtagagaa
      721 ggtcgatcgg atccttgccg agccaggcga gggcgaggcc gacggcgagc gacgcgacga
      781 ccagcttgat gacggtggcg gtggtgatgc ggggcaacgc caaggatcta tcgaccgatc
      841 gtggagaaga cgaggaacgg caggaagatg acgagtagca ggcgcatggc ggcgcgcggt
      901 tccggcgttg gattctggcc gctcgacgtg cggccgggta taggcagggc cgcagacctg
      961 cgcaagttga ggaccacgac gtgacacgac cagaagaggc gctccatctc gttgccgtcc
     1021 acgccgaggg cgagatcggc aaggtgatcg tggcgggtgc cccgacgatc ccgggccgtt
     1081 cggtgctgga caagctcgtg catctcaaca ccgtcgatga cgggctccgg cggttctgca
     1141 tcctcgagcc gcgcggcggg ccgcaggcat cggcgatcct gctcctggac ccgatcgagc
     1201 ccggcaccga cgccggcttc atcgtcatgc agcccgatac ctgccacgcc atgtcgggct
     1261 cgaacgccat ctgcgtgacc acggccctgc tcgagaccgg ccgggtggcg atgcgcgagc
     1321 cggagacccg gctggtgctc gacaccgcgg cggggccggt gccggtcctc gccacctgcc
     1381 gggccggcaa gtgcgagcgc gtgcagctcg acatgccgtg gagcttcgtc gtcgaggacg
     1441 ggctttcctt cgaggtcgag gggcagggcc gggtcgaggc cgccatcgcc ttcggcgggg
     1501 tcttctatct gctcgtcgag gcggcaccgc tcggtctcga catcgcgccc ggctcggcgc
     1561 ggcggctggt cgaggccggc atggcgatcc tcgaggaagc ccagcgtcgc tggcagccga
     1621 cccatccgga gcggccgggc atcgaaggga ttgcgtatct gatgttcatg gccgaatcgg
     1681 tcggccgcag gaccaacgcg accatcatgc cgccggggcg cgtcgaccgg tcgccttgcg
     1741 ggaccggcag ctcggcccgg ttggcgctgc tgcatgcaag gggcagggcg aatggcgcgc
     1801 gggccaccac cttccattcg atcatcggca gccagttcga ggccggcatc agtgccacgg
     1861 gcgaggtggc gggccgaccg gccatccgtc cctggatcag cgggcggggc tggatctatg
     1921 ccgaggagaa gcttctggtc gatcggcgcg acccgttcct ggctggccac gctctggccg
     1981 acgtctacgg ccccgggctg gaccgatgag cgacgaccgg acgatcgccc tctatgtcca
     2041 ctggccgttc tgccgggtca aatgccccta ttgcgacttc aacagccatg tgcgcgccga
     2101 ggtcgatcag ggccgctggc ggcgggcgct gctggccgag ctcgaccact tcgccgtcga
     2161 ggtgggaccc cggcggctga cctcgatctt ctttggtggc ggcacgccgt ccctgatgcc
     2221 gccggcgacg acggcggcgg tcatcgatcg tgccatccag catttcgtgg ccgacccggc
     2281 gctggagatc acgctggagg ccaatcccac ctcggtcgag gctggccgct ttgccgacta
     2341 tcgcacggcc ggcgtcaatc gtgcgtcgat cggcgtgcag gcgctcgatg accgcgccct
     2401 gaaggcgctc ggtcgtgagc acgacgccgc cgaggcgatc gctgcgatcg agcttgccgg
     2461 tcgcaccttc ccgcgctggt cgttcgacct gatctatgcc cgcccggggc agaccgtcgc
     2521 ggcatggcgg gcggagctcg gtcgggtgct cgatctcgcc ggcgagcatc tctcgctcta
     2581 ccagctcacc atcgagccgg gcacggtctt ccatgcgcgg caacgggcgg ggctgctcgt
     2641 gacccctcgc gacgaccggc aggcggccct tttcgagctc acgcaggagc tcaccgatgc
     2701 cgccgggttc ggggcctacg aagtctcgaa tcatgcaagg cccggcggcg agtgccggca
     2761 caacctgacc tactggcgct acgagcccta tatcggcatc ggccccgggg ccttcggccg
     2821 gctgccggcg ggcgcggggc gggtcggcac cgccacgctg cgggcacccg aggcatggct
     2881 tcgagcggtc gagggccggg gccacggcga gctgcagcgc gaggtgctgg cgcccgagga
     2941 tctcgtggtc gaggcgctga tcaccgggct ccggctcacc gaaggcgtgc ctgaggctcg
     3001 gctggcagca ctcgatccgg actggcgaac ccgtctcgat ccgcgcgccc gggttcgcct
     3061 ccaggaggcg gagcatctgg acagcgaggc tggagcgctg cggctgaccg gcgccgcgcg
     3121 cctctgcctc gacgcggtgc tgcgcgagct cgtggccgtg ccggcctgaa agaaagggaa
     3181 aagcccccga ccgcgaggcc gggggctcgt ccgtctcgaa ccgtgaagtg gttcagccgc
     3241 cgatgcaggt gtccgcgttc tcgggcgagc agacatcgag gccggtgtag gtcggatcct
     3301 cggggttctt gccgtccttg acgatgtcac ggaggaaata catcgactgg tagcccatct
     3361 cgaagggccg ctggccgacc tggccgagcg acaggccctc ggccatcagg tccatctgga
     3421 ccggcagggt gtcggcgacg acgagggcga gctcgccgct ctcgagccgg cccttgtgcg
     3481 gggtgaccgc ggcgcgatag gcatcgggca cgaactgcgg gaagccgccg gtcggcacga
     3541 aggcgtcgag gtcgggctcg cgtgcaagga tgtcctcgag ctgctggttg gcgcgcatga
     3601 aatcgtcatc ggtgtagagc gggcagccgt cgatctccgt ccagccattg gcgccttcga
     3661 gccgattgcc cggcggctcg ctgctggcct cgcccgagag cgtgtcgcgg atgccctgca
     3721 tgcgctcgtt gtggttggcg gccgccgctc cgccggactg gatgcagatg gtcccgccgt
     3781 ccggcttcag ctccatgacg cgcttcgcca ggttgacgcc gatgtcgtag ttcttggtgc
     3841 cgacataggc gatgcgcagg tccttgttct c
//
LOCUS       DAOTFI010000341         3844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_28096_length_3844_cov_7.525732, whole genome shotgun sequence.
ACCESSION   DAOTFI010000341 DAOTFI010000000
VERSION     DAOTFI010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28096_length_3844_cov_7.525732"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="P5558_22600"
     CDS             <1..328
                     /locus_tag="P5558_22600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY27172.1"
                     /translation="RELYEAARIDGATTLQEFRHITLPSMKPVFTVVVMLSTIWTSTN
                     VVFVLLLTNGGPANATQILPNLAYKFALLAGRLGTGSAVNMIFFPILAVLIVILSRKM
                     LQERMR"
     gene            366..1211
                     /locus_tag="P5558_22605"
     CDS             366..1211
                     /locus_tag="P5558_22605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY27173.1"
                     /translation="MIKTIEGQDRLWRWIGNAVLVVLVAWTLLPFYWMFATSLKKHKE
                     IYGMGTTLWPRDPTLESYRILFFETDYMLFFANSMKTAMSTTAVTVVVASLAAYAIAR
                     LDFPGRSLIARGMVYTYLVPQSLLFIPLFTILVAVGLDNSLTGLSIAYMGFTIPFCTW
                     LLMGYFMSIPVELEEAALVDGCSRLGVLVRIILPISLPALAVIAFFSFTLAWNEYLYA
                     VVFNSNPSVRTIPTGLNDFITEDVFFWGPIMGSTVLTVIPPLIVYFIFQRWLVTGLTM
                     GAVKG"
     gene            1249..2565
                     /locus_tag="P5558_22610"
     CDS             1249..2565
                     /locus_tag="P5558_22610"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY27174.1"
                     /translation="MTKLTRRTTIKAAAASAIAAPMIIRPYAAYAQRAERLVYWHLPM
                     FTPLADQAAQDLFDEYLELAGVKPEEAAFITVNNADLIPKLSAGLETGSPPDAARLYE
                     SYVQLYRAQGHMMDVTDMVEDMKAEEGGLFESSLRAVAKDGSYWGVPFAINPWPMHAR
                     NDILQQAGLAYPATWDEFIETCKTIQQPPFYGFGMDLGLTADATDNIMQLLWCYGGYT
                     TDDDGNVAFDNPGNVEGFKVIDRMYNEHKIIPKGVVGNADTAWNNKAFQSGQVAFINN
                     PTSVYAYLLDKDPEMAANTGLYSVPAGPAGSVNQIDTWSTGLFRQAPEPELVRGWVAY
                     LMDPANYSKMLLQTNGRFVPVYPKQFDDEWWTSRPEYSEFRKIAETGVPVSYKGAPSA
                     ASGEVLATHIIPAAVQSMIIDGVAAEDAVAAAHVKIKDVYERLGDG"
     gene            complement(2664..2969)
                     /locus_tag="P5558_22615"
     CDS             complement(2664..2969)
                     /locus_tag="P5558_22615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRY27175.1"
                     /translation="MPTKSAPHPGLALKRDIEAQGLSNARAAQALGVSRSQLHRVTSG
                     DCAISPDLALRLEFVFGSTAAHWLRLQADHDAARVRRRAKEITRGLERFVPAHKAPA"
     gene            complement(3063..>3844)
                     /locus_tag="P5558_22620"
     CDS             complement(3063..>3844)
                     /locus_tag="P5558_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HRY27176.1"
                     /translation="ATGGLSFNGAFVDQLVDREANEEAAAFVREKIREIVHDPETAAR
                     LSPDTIIGCKRLCAEIGFYEAFNRPNVELIDIRKEPIEAVTETGLRAGGRDFAVDAIV
                     LATGFDAMTGSFVRVDPVGVGGRHLRDAWAEGPRNYLGLAVSGFPNLFTVTGPGSPAV
                     FSNVIMSIEHHVEWIADCLAAMRESGKTRIEATKEAETDWVSHVAEVAGKTLLLGCNS
                     WYLGANIPGKPRVFMPYVGCPPYREICTRVAAEGYRGFALT"
BASE COUNT          616 a         1332 c         1226 g          670 t
ORIGIN      
        1 tagggagctc tacgaggcgg cccgcatcga cggtgccacc accctgcagg aattccggca
       61 catcaccctg ccgtcgatga agcccgtctt caccgtcgtc gtcatgctct cgaccatctg
      121 gacctcgacc aatgtcgtct tcgtcctcct cctgaccaat ggcgggccgg ccaacgccac
      181 ccagatcctg cccaatctcg cctacaagtt cgcgctcctg gccggccggc tcggcaccgg
      241 ctcggcggtc aacatgatct tcttcccgat cctggcggtg ctcatcgtca tcctgtcgcg
      301 caagatgctg caggagcgga tgcgatgagc gcggcgctga ccatcgcccg gcgggggacg
      361 ccgtcatgat caagaccata gagggtcagg accggctgtg gcgctggatc ggcaatgccg
      421 tgctggttgt cctggtcgcc tggacgttgc tgccgttcta ctggatgttc gccacctcct
      481 tgaagaagca caaggagatc tacggcatgg gcaccacgct ctggccgcgc gacccgacgc
      541 tcgagagcta ccgcatcctg ttcttcgaga ccgactacat gctgttcttc gccaacagca
      601 tgaagaccgc gatgtcgacc accgccgtca ccgtcgtcgt cgcctcgctc gcggcctacg
      661 ccatcgcccg gctcgacttt cccggccgct cgctgatcgc ccgcggcatg gtctacacct
      721 acctcgtgcc gcaatcgctg ctgttcatcc cgctcttcac catcctggtc gcggtcggcc
      781 tcgacaactc gctcaccggc ctgtcgatcg cctacatggg cttcaccatc cccttctgca
      841 cgtggctgct gatgggctac ttcatgtcga tcccggtgga gctcgaggag gcggccctgg
      901 tcgacggctg ctccaggctc ggcgtgctgg tgcgcatcat cctgccgatc tcgctgccgg
      961 cgctggcggt catcgccttc ttctccttca ccctcgcctg gaacgagtat ctctatgcgg
     1021 tggtgttcaa ctccaacccg agcgtgcgga cgatcccgac cgggctgaac gacttcatca
     1081 cggaggacgt cttcttctgg gggccgatca tgggctcgac cgtgctcacc gtgatcccgc
     1141 cgctcatcgt ctacttcatc ttccaacgct ggctcgtgac cgggctcacc atgggcgcgg
     1201 tcaagggctg aacgttcccc accaacccag aaacgacgga ggccgaccat gaccaagctc
     1261 acgaggcgca cgacgatcaa ggccgcggcg gcgagcgcca tcgccgcgcc gatgatcatc
     1321 cgcccctacg cggcctatgc ccagcgtgcg gagcggctgg tctactggca cctgccgatg
     1381 ttcacgcctt tggccgacca ggcggcgcag gatctcttcg acgagtacct cgagctggcc
     1441 ggcgtcaagc cggaggaggc ggcgttcatc accgtcaaca atgccgacct catccccaag
     1501 ctctcggccg gcctcgagac cggcagcccg cccgacgccg cgcgtctcta cgagagctac
     1561 gtccagctct accgtgccca ggggcacatg atggacgtga ccgacatggt cgaggacatg
     1621 aaggccgagg agggcggcct gttcgagagc tcgctgcggg ccgtcgccaa ggacggcagc
     1681 tattggggcg tgcccttcgc catcaatccc tggccgatgc atgcccgcaa cgatatcctg
     1741 caacaggctg gtctcgccta tcccgccacc tgggacgagt tcatcgagac ctgcaagacc
     1801 atccagcagc cgcccttcta cggcttcggc atggatctgg gcctgaccgc cgacgccacc
     1861 gacaacatca tgcagctcct ctggtgctac ggcggctaca cgacggacga cgacggcaac
     1921 gtcgccttcg acaacccggg caatgtcgag ggcttcaagg tcatcgacag gatgtacaac
     1981 gagcacaaga tcatccccaa gggcgtcgtc ggcaacgccg acaccgcctg gaacaacaag
     2041 gccttccagt cgggccaggt ggccttcatc aacaacccca cctccgtcta tgcctatttg
     2101 ctcgacaagg accccgagat ggccgcgaac accggcctct attccgttcc cgccggcccg
     2161 gccggctcgg tcaaccagat cgacacgtgg tcgaccggcc tcttcaggca ggcacccgag
     2221 cccgagctgg tccgtggctg ggtcgcctac ctgatggacc cggcgaacta cagcaagatg
     2281 ctgctccaga ccaacgggcg cttcgtgccg gtctacccca agcagttcga cgacgaatgg
     2341 tggacctcgc ggccggaata cagcgagttc cgcaagatcg ccgagaccgg tgtgccggtc
     2401 tcctacaagg gtgctccctc cgccgcctcg ggcgaggtgc tggccaccca catcatcccg
     2461 gcggccgtgc agtcgatgat catcgacggc gttgccgccg aggacgcggt ggccgcggcg
     2521 cacgtcaaga tcaaggacgt ctacgagcgc ctcggcgacg gctgacgcgc gacgtcgggc
     2581 acgagcgggg gcttcgcccg cgctcgtgcg gccacgcggc caatcaaagg tgcctgaccc
     2641 ctttgatcaa ccggcttccg ccatcaggcg ggcgctttgt gcgccggcac gaagcgctcg
     2701 aggcctctgg ttatttcctt ggcacgacgg cgcacgcggg cggcgtcgtg gtcggcctgc
     2761 aggcgcagcc agtgggcggc ggtgctgccg aagacgaact cgagcctgag cgccaggtcg
     2821 ggcgagatgg cgcagtcgcc gctggtgacg cgatgcagtt gcgagcggct gacgccgagg
     2881 gcctgcgccg cccgggcgtt ggagaggccc tgggcctcga tgtccctttt gagggcgagg
     2941 ccggggtggg gcgcgctttt cgtcggcatc ggctcgatcg cctttcgctg gtggtcgtcc
     3001 acgtcacacg gggatcatac cagcgcgtcg cgcgacatgc gatttctcgt gctctcccgt
     3061 gctcaggtca gcgcgaagcc cctatagccc tccgccgcca cccgcgtgca gatctcgcgg
     3121 tagggcgggc agccgacata gggcatgaag acccggggct tgcccgggat gttggcgccg
     3181 agataccagg agttgcagcc caggagcagc gtcttgccgg cgacctcggc gacgtggctg
     3241 acccagtcgg tctcggcctc ctttgtcgcc tcgatgcgcg tcttgccgga ttcgcgcatg
     3301 gcggcgaggc agtcggcgat ccattcgacg tggtgctcga tcgacatgat gacgttgctg
     3361 aagacggcgg ggctgccggg gccggtgacg gtgaagaggt tggggaagcc cgagacggcg
     3421 aggccgaggt agttccgcgg gccctcggcc caggcgtccc tcaggtgccg gccgccgacg
     3481 ccgaccgggt cgactcggac gaagctgccg gtcatggcat cgaagccggt ggccagcacg
     3541 atggcgtcga cggcgaagtc gcggccgccg gcccgaaggc cggtttcggt caccgcctcg
     3601 atcggctcct tcctgatgtc gatcagctcg acattggggc ggttgaaggc ctcgtagaag
     3661 ccgatctcgg cgcagaggcg cttgcagccg atgatggtgt cgggggaaag cctggccgcg
     3721 gtctcggggt cgtggacgat ctcccggatc ttctcccgga cgaaggccgc ggcctcctcg
     3781 ttggcctcgc ggtcgacgag ctggtcgacg aaggcgccat tgaagctgag gccgcccgtg
     3841 gccc
//
LOCUS       DAOTFI010000342         3842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_28114_length_3842_cov_7.235807, whole genome shotgun sequence.
ACCESSION   DAOTFI010000342 DAOTFI010000000
VERSION     DAOTFI010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28114_length_3842_cov_7.235807"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..935)
                     /gene="flgA"
                     /locus_tag="P5558_22625"
     CDS             complement(168..935)
                     /gene="flgA"
                     /locus_tag="P5558_22625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRY27177.1"
                     /translation="MTRHPGTICAPALRLWPLAAWLAVAWLAAPALAASWLVPGEPLT
                     EAHLAALVEAELPPHGDGFELSFTSPSLPLTNPAASRASLELVDLRFDLRSERFVGGL
                     LVRLETGEQRILDVGGRAQVMAEVMVPTRPVAAGERLDPAWLEPALVPARLLRADALG
                     SLDELDGTEARRRLAAGRPIRQADVQPTRLVRRGETVELRYRAPGIELVTLGRALEDG
                     ARGAFVQVANLDSERQLVARVVGSQLVEIAGRPGIGR"
     gene            complement(932..1717)
                     /gene="flgG"
                     /locus_tag="P5558_22630"
     CDS             complement(932..1717)
                     /gene="flgG"
                     /locus_tag="P5558_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRY27178.1"
                     /translation="MRSMSVAATGMMAQQLNVEVISNNIANMTTTGFKRSRPEFQDLL
                     YQTLRRGGSASSQDGTIVPAGVQVGLGVKTASVYRVMEQGNLVNTENRLDIAIQGQGH
                     FIVELPSGEFGYTRSGNFQLSPDGEIVTADGYPVSPGIVVPPDTIEVSINADGMVTAK
                     IEGEIALRDLGQLELATFVNPTGLEAVGDNLFLETEASGGAAFATPGTAGYGTVLQGF
                     LESSNVNPVQEITSLITAQRAYDMNSKVISASDEMMMTVTQLR"
     gene            complement(1769..2485)
                     /gene="flgF"
                     /locus_tag="P5558_22635"
     CDS             complement(1769..2485)
                     /gene="flgF"
                     /locus_tag="P5558_22635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRY27179.1"
                     /translation="MDNTSYVALSRQVALERQMAVVAGNIANMNTDGFKNEQVLFEDV
                     LERAGEAGRVAFVRDSGLIRDPAPGPIETTGNPLDLAINGEGYFTVETANGPRYTRGG
                     HFVLGAAGEITTSGGQPLLDEGGGTLVVPQGSGPIEVARDGTVSTPDGILGRIGLVRF
                     ADERGLRREGDSLMRTDQAPEPVPLPDIAQGRIERSNVQPVLEMTRMMETVRAFQNTQ
                     RLLETHHEMVRRVIDAASQA"
     gene            2724..3284
                     /locus_tag="P5558_22640"
     CDS             2724..3284
                     /locus_tag="P5558_22640"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRY27180.1"
                     /translation="MAEETTGEVEAEGKPRSGKKKLLLFGGIGLVLLLGVAGGAAWFL
                     GLLGGDASADMAADEAGAHATAGDGHGGDGHGGGGHGDEAPDVVFVDMPDVLVNLQSG
                     GQRIRFLKLRAALEVGSEDVATRVEALMPRIMDSFQLYLRALTVEEVSGSGGLQRLKE
                     ELAARVNYAVQPTRVDDVLVKEMLVQ"
     gene            3379..>3842
                     /locus_tag="P5558_22645"
     CDS             3379..>3842
                     /locus_tag="P5558_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRY27181.1"
                     /translation="MTGRTAASSDEPDIQVTEWDEDGADDADGATPATAAGPASRVLN
                     QDEIDSLLGTGQGKDDDERQGILALINSNDVTYERLPMLEVVFDRLERMLTTSVRNFT
                     SENVDINLDNITAQRFGDYLNSVPLPAMIAVFKAVEWDNFGLVTVDSPMIYS"
BASE COUNT          566 a         1309 c         1389 g          578 t
ORIGIN      
        1 ctcgggcggc ggcatcggca ccgagacggg ctggtagccg ggccgctcga ccgggttctc
       61 gaccggctgc aagggcggcg gcttgccgac ctgctcgagc cgctccagcg tgtagcagcc
      121 ggcgagccag agcccggcca ggagggcggc gccgagcggg cgcattctca tcgcccgatc
      181 cccggccggc cggcgatctc gaccagctgc gagccgacca cgcgggcgac gagctgccgc
      241 tcgctgtcga ggttggcgac ctggacgaag gcgcccctgg cgccgtcctc gagcgcgcgg
      301 cccagggtca ccagctcgat ccccggtgcc cggtagcgca gctcgaccgt ctcgccgcgc
      361 cgcaccagcc gcgtcggctg gacatcggcc tgccggatcg gccgcccggc cgcgaggcgc
      421 cgccgggcct cggtgccgtc gagctcgtcg agcgagccca acgcatccgc ccggagcagg
      481 cgcgccggca ccagcgccgg ctccagccag gcgggatcga gccgctcgcc cgccgccacc
      541 ggccgcgtcg gcaccatgac ctcggccatg acctgggccc ggccgccgac gtcgagaatc
      601 cgctgctcgc cggtctcgag ccgcaccagc aggccgccga cgaagcgctc gctgcgcaga
      661 tcgaagcgca gatcgaccag ctccaggctg gcgcggctcg ctgccgggtt ggtcagcggc
      721 aggctcggtg aggtgaagct cagctcgaag ccgtcgccgt gcggcgggag ctccgcctcg
      781 acgagcgccg ccagatgcgc ctcggtgagc ggctcgcccg gcaccagcca ggaggcggcg
      841 agggccggtg ccgccagcca tgcgacggcg agccaagcgg cgagcggcca gagccgcagg
      901 gcgggtgcgc agatcgtgcc ggggtgccgg gtcatcgcag ttgtgtcacc gtcatcatca
      961 tctcgtcgga ggccgagatc accttggagt tcatgtcata ggcccgctgg gcggtgatca
     1021 ggctggtgat ctcctgcacc gggttgacgt tggagctctc gaggaagccc tgcagcacgg
     1081 tgccgtagcc ggccgtgccg ggcgtggcga aggcggcacc gcccgaggcc tcggtctcga
     1141 ggaagagatt gtcgccgacc gcctcgagcc cggtcgggtt gacgaaggtg gcgagctcga
     1201 gctggccgag gtcccgaagc gcgatctcgc cctcgatctt ggcggtgacc atgccgtcgg
     1261 cattgatcga gacctcgatc gtgtcgggcg gcacgacgat gcccgggctc accgggtagc
     1321 cgtccgcggt gacgatctcg ccgtcggggc tgagctggaa attgcccgag cgggtgtagc
     1381 cgaactcgcc ggacggcagc tcgacgatga agtggccctg gccctggatc gcgatatcga
     1441 gccggttctc ggtgttgacc aggttgccct gctccatcac gcggtagacc gacgcggtct
     1501 tgacgccgag gccgacctgc acgccggccg gcacgatcgt gccgtcctgc gacgaggcgg
     1561 agccgccgcg ccgcagcgtc tggtagagca ggtcctggaa ctccggccgc gaccgcttga
     1621 agccggtcgt cgtcatgttg gcgatgttgt tggagatgac ctcgacgttc agctgctggg
     1681 ccatcatgcc ggtggcggcg acactcatcg agcgcatggc ggactactcc atgaatgcgg
     1741 ggtatgggcg cgttcttcgg tcatcggctc aggcctggct cgcggcgtcg atcacccggc
     1801 ggaccatctc gtgatgcgtc tcgagcaggc gctgcgtgtt ctggaaggcg cggacggtct
     1861 ccatcatccg ggtcatctcg agcaccggct ggacgttgga gcgctcgatc cggccctggg
     1921 cgatgtccgg caagggcacc ggctccggcg cctggtcggt gcgcatcagg ctgtcgccct
     1981 cgcgccggag accccgttcg tcggcgaagc gtaccagtcc gatccggccg agaatgccgt
     2041 cgggagtcga gaccgtgccg tcgcgcgcca cctcgatcgg tccgctgcct tgcggcacga
     2101 ccagcgtccc gccgccctcg tcgagcagcg gctggccgcc gctggtggtg atctcgccgg
     2161 cggcgcccag gacgaaatgg ccgcccctcg tgtagcgggg accgttcgcc gtctcgaccg
     2221 tgaagtagcc ctcgccattg atcgcgagat cgagcgggtt gccggtcgtc tcgatcggcc
     2281 ccggggccgg atcgcggatc aggccggaat cccggacgaa agcgacgcgg ccggcctcgc
     2341 cggcgcgctc gagcacatcc tcgaacagca cctgctcgtt cttgaatccg tcggtgttca
     2401 tgttggcgat attgcccgcc acgaccgcca tctggcgttc gagggcgacc tgtcggctca
     2461 gcgccacgta gctcgtattg tccatgcgtc ccgattctct ggggcccggt gtcgccggat
     2521 ctcatgcacg ccccgtgcca gtcgcccgga gccgggagtt ccggggctcc ccggcatccg
     2581 gtggcccgtc cccggcaatt tcgaccgggc aattcctgcc gggccggcag ccccggaccg
     2641 gtcggcagac ggctgcgcaa ggcttcgtta accagtgccg gctacaaagg cggtcgcgac
     2701 agttgccgag gacggattcg agcatggccg aagagacgac gggcgaagtc gaggctgagg
     2761 gaaagccgag gtcgggcaag aagaagctcc tgctcttcgg cggcatcggg ttggtgctgc
     2821 tcctgggtgt cgccggcggt gcggcctggt tcctcgggct gctcggcggc gatgcgtcgg
     2881 ccgacatggc ggcggacgaa gccggcgcgc atgccacggc cggggacggt catggcggcg
     2941 atggccatgg cgggggcggg catggcgacg aggcgcccga cgtcgtcttc gtcgacatgc
     3001 cggacgtgct ggtcaacctg cagtccggcg gccagcgcat ccgcttcctc aagctcaggg
     3061 cggcgctcga ggtcggaagc gaggatgtgg cgaccagggt cgaggccctg atgccccgca
     3121 tcatggacag cttccagctc tatctgcgcg ccctcacggt cgaggaggtg agcggttccg
     3181 gcgggctgca gcggctcaag gaggagctcg ccgcgcgggt caattacgcg gtgcagccaa
     3241 cccgggtcga tgacgtgctc gtcaaggaga tgctggtcca gtagccgggc cggcgatgat
     3301 ggagatggcg ggacgtggtg aggggcgtcg accgatgacg agatcgaccg cgtggtcgcg
     3361 gccggagcgg gtgaggcgat gaccggcaga acggccgctt cgtctgacga gcccgacatc
     3421 caggtcaccg agtgggacga ggacggtgcg gacgacgccg acggcgccac gccggccacg
     3481 gccgccggcc cggccagccg ggtcctcaat caggacgaga tcgacagcct cctcggcacc
     3541 gggcagggca aggacgacga cgagcgccag ggcatcctgg cgctgatcaa ctccaacgac
     3601 gtcacctacg agcgcctgcc gatgctcgag gtggtgttcg accggctcga gcgcatgctg
     3661 accacgagcg tgcgcaactt cacctcggaa aatgtcgaca tcaacctcga caatatcacc
     3721 gcccaacggt tcggcgacta cctcaattcc gtgccgctgc cggcgatgat cgccgtcttc
     3781 aaggcggtcg agtgggacaa tttcggcctg gtcaccgtcg acagcccgat gatctactcg
     3841 at
//
LOCUS       DAOTFI010000343         3810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_28418_length_3810_cov_6.201065, whole genome shotgun sequence.
ACCESSION   DAOTFI010000343 DAOTFI010000000
VERSION     DAOTFI010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28418_length_3810_cov_6.201065"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="P5558_22650"
     CDS             complement(<1..579)
                     /locus_tag="P5558_22650"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRY27182.1"
                     /translation="MSKSPTAKSPTERLEACYTGIIHDVMRAMGLKGFVLPPEIRPLF
                     PEQKLAGPIFTVKGRVDETADAHTTLLEWTGLLAEAKPGHVVVCEANNTVTAMMGELS
                     AETLQYRGVRGYIVDGACRDVDFILKIGFPVCFTHYTPRDIVAYWRPSGFEVPIRIGD
                     VDIRPGDYVLADRDGIVVIPKDRIEPVLDAAET"
     gene            complement(591..1769)
                     /locus_tag="P5558_22655"
     CDS             complement(591..1769)
                     /locus_tag="P5558_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY27183.1"
                     /translation="MRIEAVDLFYVAMPEVTDAGDGSQDALLVRVAAGGLTGWGECEA
                     SPLPSIAAFVCPKSHGACKPIADSVLGQSVETPADIARIAHTIAWDSMDLLQAPHTWS
                     GIEMALWDLIGKARGEPVWRLLGYDRAHPKTPYASQLWGDTPQDTRDACQAQIAAGFR
                     AVKCGWGPFGRGTLQDDVDQLHAAREGLGPEGILLVDAGAIFGEDIEAAAARLPALEA
                     VNATWYEEPVAGMAYEAYAAIGARSSKVRLAGGEAAHNLFMARHTIDYGKVGYVQIDC
                     GRIGGIGPAKAVAEHAVARGVTYVNHTFTSNLALSASLQPFAGLEDHRICEFPVHTRP
                     VAKAFCATRIEPDARGLIHVPDAPGLGVAIDPKGIRDYLLDVEIRVGGRTLYSTPSPD
                     "
     gene            complement(1781..2983)
                     /locus_tag="P5558_22660"
     CDS             complement(1781..2983)
                     /locus_tag="P5558_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY27184.1"
                     /translation="MKITALETVWLEEFPNLCWVQVHTDEGLVGLGETFFGPKAVIAY
                     IHETAAPRLIGKDPLAIDRHSRTLIHDYYLGFAGSGAEMRGASAVDIALWDIWGKATS
                     QPVHQLLGGLSRPRIRTYNTCAGYRYVRGTKGQMTQNWGLDARQGPYEDLDAFLNHAG
                     DLALSLQEEHITGMKIWPFDFAAERTDGSYISPEELRTALEPFAKIRDAVGDGMDIMV
                     ELHSLWNLPAAKRILAALEPYQPFWYEDPIKMNNFDALAELAASTRVPITASETIATR
                     PMFRELMAKRAVGVVMLDLAWCGGLSEAKKIATMAEAHQLPVAPHDCTGPIVYTASCH
                     LSLNAPNALIQESVRAFYTGWYKELVTELPRMEKGHILPMAGPGLGTELLPDVRKRPD
                     AVVQVSKD"
     gene            complement(3024..>3810)
                     /locus_tag="P5558_22665"
     CDS             complement(3024..>3810)
                     /locus_tag="P5558_22665"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY27185.1"
                     /translation="TDAFVAKARFVQARDDVYDLEPSHSAAEPRVRRIKTPHLQHDAY
                     ARIGRHPAIVEILRQLVHPVLRFDTSKLNMKAAGYGAAVEWHQDWAFYPHTNDDLAAV
                     GVMMDDCAIENGPLLCVPGSHKGPIHEHHADGVFCGAIDLDQADVDFAAARPCVGPAG
                     SISIHHARTIHGSALNTSQKMRRLLLFQYRAADAWPLVGSQQPKSWADWQALLLSGEN
                     DPVVPRCTDVPVRLPLPEAKHAGSIYENQRGLKKGYFGQAPAA"
BASE COUNT          531 a         1199 c         1452 g          628 t
ORIGIN      
        1 ggtctcggcg gcgtcgagga cgggctcgat cctgtccttc gggatgacga cgatgccgtc
       61 gcggtcggcg aggacgtagt cgccgggccg gatgtcgaca tcgccgatgc ggatcggcac
      121 ctcgaagccc gaggggcgcc agtaggcgac gatgtccctc ggcgtgtagt gggtgaagca
      181 gacggggaag ccgatcttca ggatgaagtc gacgtcgcgg caggcgccgt cgacgatgta
      241 gcccctgacc ccgcgatatt gcagcgtctc ggcggagagc tcgcccatca tcgccgtgac
      301 ggtgttgttc gcctcgcaga cgacgacgtg gccgggcttg gcctcggcga ggaggccggt
      361 ccattcgagg agcgtggtgt gggcgtcggc ggtctcgtcg accctgccct tcacggtgaa
      421 gatggggccg gcgagcttct gctcggggaa gagcgggcgg atctcgggcg gcaggacgaa
      481 gcccttcagc cccatggccc gcatcacgtc gtggatgatg ccggtgtagc aggcttcgag
      541 gcgctcggtc gggctcttgg cggtcgggct cttgctcatg gcggctccgg tcagtctggc
      601 gatggcgtgc tgtagagggt ccggccgccg accctgatct cgacgtcgag gaggtagtcc
      661 ctgatgccct tcgggtcgat ggcgacgccg aggcccgggg cgtccggcac gtggatcagg
      721 ccacgggcgt ccggctcgat gcgggtggcg cagaacgcct tggcgacggg ccgcgtatgc
      781 accgggaatt cgcagatgcg atgatcctcg agcccggcga acggctgcag cgaggccgag
      841 agggcgaggt tcgaggtgaa ggtgtggttc acgtaggtga cgcccctggc caccgcgtgc
      901 tcggccaccg ccttggccgg gccgatgccg ccgatccggc cgcagtcgat ctggacgtag
      961 ccgaccttgc cgtagtcgat ggtgtggcgg gccatgaaga gattgtgggc ggcctcgccg
     1021 ccggcgagcc gcaccttcga gcttcgagca ccgatggcgg cataggcttc gtaggccatg
     1081 ccggcgaccg gctcctcgta ccaggtggcg ttcaccgcct cgagcgccgg caggcgggcg
     1141 gcggcggcct cgatgtcctc gccgaagatg gcgccggcat cgacgagcag gatgccttcc
     1201 ggcccgagac cctcgcgggc ggcgtggagc tggtcgacgt cgtcctgcag ggtgccgcgg
     1261 ccgaaggggc cccagccgca cttgacggcg cggaagccgg ccgcgatctg tgcttggcag
     1321 gcgtcgcgcg tgtcctgcgg cgtgtcgccc cagagctgcg aggcataagg ggtcttggga
     1381 tgcgcccggt catagcccag gagccgccag accggctcgc cgcgcgcctt gccgatgaga
     1441 tcccagagcg ccatctcgat gccggaccag gtatgcggcg cctgcagcag gtccatgctg
     1501 tcccaggcga tggtgtgggc aatccgtgcg atatcggccg gcgtctcgac cgactggccc
     1561 agcaccgaat cggcgatcgg cttgcaggcg ccgtgcgact tcgggcagac gaaggcggcg
     1621 atcgacggca aaggcgaggc ctcgcattcg ccccagccgg tgagcccgcc ggcggcgacg
     1681 cgcaccagga gcgcatcctg gctgccgtcg ccggcatcgg tgacctccgg catggcgacg
     1741 tagaagagat cgacggcttc gatgcgcatg ttgctggccc tcaatccttg ctcacctgga
     1801 cgaccgcgtc cgggcgtttc ctgacatccg gcaggagctc ggtgccgagg ccggggccgg
     1861 ccatgggcag gatgtggccc ttttccatgc gcggcagctc ggtcaccagc tccttgtacc
     1921 agccggtgta gaaggcgcgc acgctctcct ggatcagcgc gttcggggcg ttgagggaga
     1981 ggtggcagga ggcggtgtag acgatcgggc cggtgcagtc gtggggggcc acgggaagct
     2041 ggtgggcctc ggccatggtg gcgatcttct tggcctcgga gaggccgccg caccaggcga
     2101 ggtcgagcat gacgacgccg acggcgcgct tcgccatgag ctcgcggaac atcgggcggg
     2161 tggcgatggt ctcgctcgcc gtgatcggca cccgggtcga cgccgccagc tcggccagcg
     2221 cgtcgaaatt gttcatcttg atcgggtcct cgtaccagaa gggctggtag ggctcgaggg
     2281 ccgccaggat gcgcttggcg gccggcaggt tccagagcga gtgcagctcg accatgatgt
     2341 ccatgccgtc gccgacggcg tccctgatct tggcgaacgg ctcgagcgcc gtccgcagct
     2401 cctcggggct gatgtagctg ccgtcggtgc gctcggcggc gaagtcgaag ggccagatct
     2461 tcatgccggt gatgtgctcc tcctggagcg acagcgcgag atcgccggca tggttgagga
     2521 aggcgtcgag gtcctcgtag ggcccctggc gagcgtcgag gccccagttc tgggtcatct
     2581 gtcccttggt gccgcgcacg tagcggtagc cggcgcaggt gttgtaggtg cggatcctgg
     2641 gccgcgacag cccgcccagg agctggtgca ccggctggct cgtggccttg ccccagatgt
     2701 cccagagcgc gatgtcgacc gccgaggcgc cgcgcatctc cgccccgctg ccggcgaagc
     2761 ccagatagta gtcgtggatc agcgtgcgcg aatggcggtc gatggcgagc gggtccttgc
     2821 cgatcagcct gggtgccgcc gtctcgtgga tgtaggcgat caccgccttc gggccgaaga
     2881 aggtctcgcc gagcccgacg aggccctcgt cggtatggac ctggacccag cacaggttcg
     2941 ggaactcctc gagccagacg gtctcgaggg cggtgatctt catggcggcg ttcctatgtt
     3001 ggtcggcggg gttccggcag cggtcaggcg gcgggggcct ggccgaaata gcccttcttc
     3061 aggccgcgct ggttctcgta gatcgagccc gcgtgcttcg cctccggcaa gggcaggcgg
     3121 accggcacgt cggtgcagcg cggcaccacc ggatcgttct cgccgctgag cagcagcgcc
     3181 tgccagtcgg cccagctctt cggctgctgg ctgccgacga gcggccaggc gtcggcggcg
     3241 cggtactgga agagcaggag gcggcgcatc ttctgcgacg tgttgagggc cgagccgtgg
     3301 atggtccggg catggtggat cgagatgctg ccggccgggc cgacgcaggg tcgggccgcg
     3361 gcgaagtcga catcggcctg gtcgaggtcg atcgcaccgc agaagacgcc gtcggcatgg
     3421 tgctcgtgga tggggccctt gtggctgccc ggcacgcaga gcagcggccc gttctcgatg
     3481 gcgcagtcgt ccatcatgac gccgacggcg gcgaggtcgt cattggtgtg cgggtagaag
     3541 gcccagtcct ggtgccactc gacggcggca ccgtagccgg ccgccttcat gttgagcttg
     3601 gaggtgtcga agcgcagcac ggggtggacg agctgccgca ggatctcgac gatcgccggg
     3661 tgccggccga tcctggcata ggcgtcgtgc tggagatgcg gcgtcttgat ccggcgcacc
     3721 cgcggctcgg cggcgctgtg gctgggctcg aggtcgtaga cgtcgtcgcg ggcctggacg
     3781 aagcgggcct tggcgacgaa cgcgtcggtg
//
LOCUS       DAOTFI010000344         3800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_28512_length_3800_cov_5.021362, whole genome shotgun sequence.
ACCESSION   DAOTFI010000344 DAOTFI010000000
VERSION     DAOTFI010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28512_length_3800_cov_5.021362"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="P5558_22670"
     CDS             complement(<1..269)
                     /locus_tag="P5558_22670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27186.1"
                     /translation="MRLYPASLAFNAGELSPLLEGRADLDFYSIGGAAMVNWLPTVEG
                     PMRRRPGFELKGGPLGFNIGDEPKRGIVLGRFAFRADQSYLLEVG"
     gene            complement(269..472)
                     /locus_tag="P5558_22675"
     CDS             complement(269..472)
                     /locus_tag="P5558_22675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27187.1"
                     /translation="MSAWVKEETRGGVVHVEAGGDDRRAVQAAVSDYLRRWPPAGYDT
                     RFGTVARSGDGFRAVGSRLRSCD"
     gene            complement(469..1047)
                     /locus_tag="P5558_22680"
     CDS             complement(469..1047)
                     /locus_tag="P5558_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27188.1"
                     /translation="MATTIDLLNRGLLKLGCDRIESLGDDLEEARAAAAIWPTVRDFV
                     LSAAAWRFAMHRTTLATLGPTPAFGYLHALQLPSDCLRLYQAGEDLVAMRLYRGAVEG
                     GYLLTNQAAPVPVRYVRRVEDASRYHPDFVEAAACRLAAEACERLTGNRGLLELLDQQ
                     FRLALEHGRAHDAIESDPLALAETGPWVESRL"
     gene            complement(1107..1598)
                     /locus_tag="P5558_22685"
     CDS             complement(1107..1598)
                     /locus_tag="P5558_22685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27189.1"
                     /translation="MGVVATQSTAVANHLAVPAIVSDVSINGGRKRFATASVAIASGD
                     DDGSVYFLLPVHSSWVITSIKVFNDAITSGTDFNLGLYNVDLTDADENVYADAISLAT
                     ASLVGVEQAFEARDITTMGRRVWEDAGEASNLGKWYLLALTGVTVGSASGDVVVDVTY
                     TVD"
     gene            complement(1602..2477)
                     /locus_tag="P5558_22690"
     CDS             complement(1602..2477)
                     /locus_tag="P5558_22690"
                     /inference="COORDINATES: protein motif:HMM:NF040375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HRY27190.1"
                     /translation="MSYEVPAHFVQQYTTNVQLLLQQRGSKLRGHVTEHGFTGKAAKA
                     VEQVGAVTAVKRTSRHADTPLIATPADARWCYPDDYGWADLIDDVDKLKMLIDPQSSY
                     ALNGSYAIGRSMDDAIIAAATGVSKAGENGTTSKSFLSTQEVAASVGSTTGMNHAKVR
                     KARELLEKAEVDVDNDPLTIALSAVQAMELFGETITGSVDYVNGRPIVTGKLPMLYGF
                     EFVCIERLAKASNDRWVLAFAKSGLHLGIWDEISTEIDRIPGKWGSHQVMVKGSFGAV
                     RSEEKKIVRILSTES"
     gene            2691..3050
                     /locus_tag="P5558_22695"
     CDS             2691..3050
                     /locus_tag="P5558_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27191.1"
                     /translation="MQWAKGQSGNPGGRPAVVAEVKRLARMHTGEAIDRLVELMRCED
                     HRVAFAAVRELLDRGYGRAPVTLAEEQPIAGPVILVTGIIRAGDRPVTLEGEAAEIDP
                     DELDPGQQADQQDEPEA"
     gene            3047..>3800
                     /locus_tag="P5558_22700"
     CDS             3047..>3800
                     /locus_tag="P5558_22700"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRY27192.1"
                     /translation="MTLEALDLFSGGAGGWSLACHRLGIRTLAACEADPARRHMYAQN
                     FPEVPIYDDVQELTAARLLADLGTLPHIVVGSPPCQDASVANPRGRGTGGKRTGLAGE
                     FVRLVGEVRPLLAVLENVPGLKGRGADWLLASLAAAGYAARPFVVGAIHAGAPHRRLR
                     CWIVAVPAAALADPDRYRLRLEQQRLPGRRPAGLCDGRQAEPRHPRPIGASDADQGRQ
                     PGERLEKRPGIEGASGLEPVRPGAHRIEHGPDD"
BASE COUNT          587 a         1318 c         1328 g          567 t
ORIGIN      
        1 tggccgacct cgaggaggta ggactggtcg gcacggaagg cgaagcggcc gagcacgatg
       61 ccgcgcttgg gctcgtcccc gatattgaag ccgagcggcc cgcccttgag ctcgaacccg
      121 ggccgacggc gcatcggccc ctcgaccgtc ggcagccagt tgaccatggc ggcgccgccg
      181 atgctgtaga aatccagatc ggcccgcccc tcgaggagcg gcgacagctc gccggcattg
      241 aaggcgagcg aggcgggata gagccgcatc agtcgcagga gcgaaggcgg gagccgaccg
      301 cgcggaaccc gtcgccgctc cgggcgacgg tgccgaagcg cgtatcgtag ccggccggcg
      361 gccagcggcg cagatagtcc gagacggcgg cctggaccgc gcggcgatcg tcgccgccgg
      421 cctcgacatg gacgacgccg ccccgggttt cttccttcac ccaggcgctc acagccggga
      481 ctccacccac gggccggttt ccgccagggc gagcggatca ctctcgatgg cgtcgtgcgc
      541 ccggccgtgc tcgagggcga gccggaactg ctggtcgagc agctcgagca gcccccggtt
      601 gccggtcagc cgctcgcagg cttcggccgc cagccggcag gccgcggcct cgacgaaatc
      661 cggatgatag cgcgaggcgt cctcgacccg gcggacgtag cggaccggga ccggtgcggc
      721 ctggttggtg agcagatagc cgccctcgac cgcgccccga tagaggcgca tggcgaccag
      781 gtcctcgccg gcctggtaga gccgcaggca gtccgagggc agttgcaggg cgtggagata
      841 gccgaaggcc ggagtcggcc cgagcgtggc gagcgtggtc cggtgcatgg cgaagcgcca
      901 ggccgcggcc gacagcacga agtcgcgcac cgttggccag atcgcggcgg ccgcccgcgc
      961 ttcttccaga tcgtcgccga ggctttcgat ccggtcacag ccgagcttga ggagcccgcg
     1021 attgaggagg tcgatggtgg tggccatcgt cagagcggcg aggccgcccg gtgccgtgca
     1081 ccaccgggcg agccccgcct accccgtcag tcgacggtat aggtgacgtc gacgaccaca
     1141 tcgccggagg ccgagccgac ggtaacgccg gtcagcgcca gcaagtacca cttgccgagg
     1201 ttcgacgcct cgccggcatc ctcccagacc cggcgcccca tggtggtaat gtcgcgcgcc
     1261 tcgaaagcct gctcgacgcc gaccaggctg gccgtggcca ggctgatcgc gtcggcgtag
     1321 acgttctcgt cggcgtcggt cagatcgaca ttgtagaggc cgagattgaa gtcggtgccc
     1381 gaggtgatcg cgtcgttgaa aaccttgatc gacgtgatca cccaggacga atgaaccggc
     1441 aggagaaagt agacactgcc atcgtcgtcc ccggaggcga tcgccaccga ggccgtggcg
     1501 aaccgcttgc ggccgccatt gatcgagacg tccgagacga tcgccggcac cgcgaggtga
     1561 ttggcgaccg ccgtggactg cgttgcgaca acacccatcg gttaggactc cgtgctcagg
     1621 atgcggacga tcttcttttc ctcgctccgg accgcgccga aggagccctt caccatgacc
     1681 tgatgcgagc cccacttgcc cgggatgcgg tcgatttcgg tgctgatctc gtcccagatg
     1741 ccgaggtgca ggccggactt ggcgaaggcc agcacccacc ggtcgttcga cgccttggcc
     1801 agccgctcga tgcagacgaa ctcgaagccg tagagcatcg gcagcttgcc ggtgacgatc
     1861 ggccggccgt tcacgtagtc gacgctgccg gtgatggtct cgccgaagag ctccatcgcc
     1921 tgcaccgccg aaagggcgat ggtcagcgga tcgttgtcga cgtcgacttc cgctttctcg
     1981 aggagctcgc gcgccttcct gaccttggcg tggttcatgc cggtggtgct gccgacgctg
     2041 gccgccacct cctgagtcga gaggaacgac ttgctcgtgg tgccgttctc gcccgccttg
     2101 gagacgccgg tggccgccgc gatgatggcg tcgtccatgc tccggccgat ggcataggag
     2161 ccgttcaggg cgtagctcga ctgcgggtcg atcagcatct tgagcttgtc gacatcgtcg
     2221 atcagatcgg cccacccgta gtcgtccggg tagcaccagc gggcgtcggc cggcgtggcg
     2281 atcaagggcg tgtcggcatg gcgcgaggtg cgcttgacgg cggtgacggc acccacctgc
     2341 tcgaccgcct tggcggcctt gccggtgaag ccgtgctcgg tgacgtggcc gcggagcttg
     2401 ctgccccgct gctggagcag cagttgcacg ttggtcgtgt actgctggac gaaatgggcc
     2461 gggacttcgt agctcatttg ggcaaccccc aatcacgatg attgaagagg ctgcccggcc
     2521 gatccggacc ccgtagcctg gacgccaggc cctcgcgccg gtctgttccc ggtgtcagcg
     2581 gaccggtgcg aaccggctcc ccgcgcagcc cggaaagata gctcggtcgc cttgcaggag
     2641 caaggcattt aatcctatac tgcggccccg aacgatgcgc ggagccggca gtgcaatggg
     2701 ccaagggcca gagcggaaac cccggcggcc ggcctgccgt cgtcgccgaa gtcaagcgcc
     2761 tggcgcggat gcacacgggc gaggccatcg accggctggt cgagctgatg cgctgcgagg
     2821 atcaccgcgt tgccttcgcg gcggttcgtg agttgctcga tcgaggctat ggccgcgccc
     2881 ccgtcacgct cgccgaggag cagccgattg ccggccccgt gatcctggtg acgggcatca
     2941 tccgcgccgg cgatcgcccg gtgacgctcg agggcgaggc cgccgagatc gaccccgacg
     3001 agctcgaccc cgggcagcag gccgaccagc aggacgagcc cgaggcatga cgctcgaggc
     3061 cctcgacctc ttttccggcg gcgccggcgg ctggtcgctg gcctgtcacc ggctcggcat
     3121 ccgcacgctg gccgcctgcg aggccgaccc ggcgcgccgg cacatgtacg cccagaactt
     3181 ccccgaggtg ccgatctatg acgacgtgca agagctcacg gccgcccgcc tgctcgccga
     3241 tctgggaaca ctgccgcaca tcgtggtggg ctcgccgccc tgccaggacg ccagcgtcgc
     3301 caaccccaga ggccgcggga ccggcggcaa gcgcaccgga cttgccggcg agttcgtccg
     3361 actcgtcggc gaggttcggc cccttctggc agtgcttgaa aacgtccctg gcctcaaagg
     3421 tcgcggcgcg gactggctgc tcgccagcct ggcggcagcg ggctacgccg cgcggccttt
     3481 cgtggtcggt gctatccatg ccggcgcacc gcaccgccgg ctccggtgct ggatcgtcgc
     3541 ggttcccgcc gccgcgcttg ccgaccccga ccgctaccgc ctacggctcg agcaacaacg
     3601 gttgcccggg cgacggccgg ccggcctatg cgacggccgg caagccgagc ctcgccaccc
     3661 tcgcccgatc ggtgcttccg acgccgacca aggccgacag ccgggcgagc ggctcgagaa
     3721 acgccccggg atcgaaggcg catccgggct tgagcctgtc cgacctggtg ctcaccggat
     3781 cgagcacggg ccggacgatg
//
LOCUS       DAOTFI010000345         3792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_28591_length_3792_cov_6.122558, whole genome shotgun sequence.
ACCESSION   DAOTFI010000345 DAOTFI010000000
VERSION     DAOTFI010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28591_length_3792_cov_6.122558"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="P5558_22705"
     CDS             complement(<1..1122)
                     /locus_tag="P5558_22705"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRY27193.1"
                     /translation="MKGETPRPHSPILGLIVNPVAGLGGAVALKGSDGAAVQAAALAK
                     GAVPRAGEKAARALLALRERLSDVEVLAFGGGMGDPSATQIGEPAEPSSGLDTRRAAV
                     ALRDAGAALLLVVGGDGTVRDVLAAVGDAVPILGAPAGVKMQSAVFATSPRHAGEVAA
                     AFLGGDRRVRLMAREVMDIDEAALRAGRLGARLHGFATVPVVEGLVQNPKAGQGPGEA
                     AALAGLCAAIAAEMIPGRLYVLGPGTTKQQVLEAFGLEGTLLGVDAVLDGKLVGRDLD
                     ATALETLVDGRPATLVVSGACWGGADGLVALDPSRFPDDNLVWSFHSYAPFILTHQGA
                     GWTGDISPYATGLPYPPYGANARETAAALKAIRARVRAEA"
     gene            1179..2645
                     /locus_tag="P5558_22710"
     CDS             1179..2645
                     /locus_tag="P5558_22710"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY27194.1"
                     /translation="MSTAPTRPERYRLAPDLEISRIVTGLWQVADMERQAGRPLDQEA
                     AAEALAVYAESGFDSFDMADHYGSAELIAGRLLAGYHKGHKPRAFTKWCPEPGPMTAE
                     VVRAGIAERLERLGVERLDLLQLHWWLYEHPAYLDAMAELARLREEGLIGELGVTNFD
                     TEHLRILMAEGYPVRTNQVSFSLLDRRAAGPLTAFCQEHGIRLLAYGTLAGGFMDERW
                     LGAKEPASIADWSRMKYRRFIDAIGGWDAFQRILAAAGAIAEKHGVKIANVATRWVLD
                     QPAVAAVIVGARPGERDHRETNLRSFAFALDAEDHARLDAAFAASRPVPGDCGDEYRR
                     PPFLTASGDLSHHLGTLPRVFEAIPDGDRSRVSSGSVWEEVAGYTRALRVGDRVLVSG
                     TTATHGTDHVVARGDPAAQTAYVLDKIQASLRAVGAGLEDVVRTRVFVRDIAQWEPVA
                     RTHGRYFRDHRPVNSLVEAKLVGDYLVEIEAEAVVRQA"
     gene            complement(2636..3469)
                     /locus_tag="P5558_22715"
     CDS             complement(2636..3469)
                     /locus_tag="P5558_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRY27195.1"
                     /translation="MAEPCIITVAITGSLPTKANNPAVPISVAEQVESTQAAFEAGAT
                     LAHCHVRDDEGKPTSDPERFGRLLEGLRKHCPGMIVQFSTGGRSGRGEERGGMLPLKP
                     DMASLSTGSVNFPTRIYENSPDLVDWLAALMLEHGVKPEIEVFDLSMLFKAVEMEKAG
                     QIRGPLHVQFVMGVKNAMPVDREVFDFYVRTVKRLAPDATWTGAGIGRDQITLVRWSL
                     EQGGHCRTGLEDNVRLDKERLAPSNAALVEQVVALCPEYGRRPATVAEARSLLGLPAQ
                     A"
BASE COUNT          586 a         1423 c         1236 g          547 t
ORIGIN      
        1 agcctcggcc ctgacgcggg cgcggatggc cttcagcgcc gcggcggttt cccgggcatt
       61 cgcgccgtag ggcggatagg gaaggccggt cgcgtagggg ctgatgtcgc ccgtccagcc
      121 ggcaccctgg tgcgtgagga tgaacggcgc gtaggagtgg aagctccaga ccaggttgtc
      181 atcggggaaa cgggacggat cgagcgctac aaggccgtcg gccccgcccc agcaggcccc
      241 cgagacgacg agcgtcgccg gccgcccgtc gaccagcgtc tccagcgccg tcgcatccag
      301 gtcccggccg accagcttcc cgtccaggac cgcgtcgacg ccgagcagcg tgccctctag
      361 gccaaaggct tccagcactt gctgcttggt tgtgcccggc ccgaggacgt agagccggcc
      421 cggaatcatt tcggccgcga tggccgcgca gaggcccgcg agtgccgccg cctcgcccgg
      481 cccctggccc gccttcgggt tctgcacgag gccctccacc accggcacgg tggcgaagcc
      541 gtggaggcgg gcgccgagcc ggccggcgcg cagcgccgcc tcgtcgatgt ccatgacctc
      601 gcgggccatg agcctcaccc gcctgtcgcc cccgaggaag gccgccgcca cctcgcccgc
      661 gtggcgcggg ctggtcgcga agaccgccga ctgcatcttc acgccggccg gcgcgccgag
      721 gatcggcacc gcatcgccca cggcggccag cacgtcgcgc accgtgccgt cgccgccgac
      781 gacgagcagg agtgccgcac cggcatcgcg cagcgccacg gcggcacgcc gcgtgtcgag
      841 cccgctgctc ggctcggccg gctcgccgat ctgggtggcc gagggatcgc ccattccgcc
      901 gccgaaggcc aggacctcga catcggacag gcgctcgcgc aaggccagga gtgccctcgc
      961 cgccttctcg ccggcgcgcg gcacggcgcc tttggccagt gctgcggcct gcaccgcggc
     1021 gccatccgag cccttgagcg ccacggcccc gccaagcccg gccacgggat tgacgatcag
     1081 acccaagatc ggcgagtggg gcctcggcgt ttcgcccttc acggtcgggc ctccaacggc
     1141 ctactctccg ccgccgccag agccgaggaa cacgcccgat gtccaccgcc ccgacccgcc
     1201 ccgaacgcta ccgcctcgct ccggatctcg agatcagccg catcgtcacc gggctctggc
     1261 aggtcgccga catggagcgc caggccggcc ggccgctcga ccaggaggcc gccgccgaag
     1321 ccctcgccgt ctacgccgaa agcggtttcg acagcttcga catggccgac cactacggct
     1381 cggccgagct gatcgccggc cgcctgctcg ccggctacca caagggccac aagccccggg
     1441 ccttcaccaa atggtgcccc gagcccgggc cgatgacggc ggaggtcgtc cgcgccggca
     1501 tcgccgagcg gctggagcgc ctcggcgtcg agcggctcga tctcctgcag cttcactggt
     1561 ggctctacga gcatccggcc tatctcgacg ccatggccga gctcgcgcgg ctccgcgagg
     1621 agggcctgat cggcgagctc ggcgtcacca atttcgatac cgagcatctg cgcatcctga
     1681 tggctgaagg ctacccggtc cgcaccaacc aggtcagctt ctcgctcctc gaccgccgcg
     1741 ccgccggccc gctcacggcc ttctgccagg agcacggcat ccggctcctg gcctacggca
     1801 cgctcgccgg cggcttcatg gacgagcgct ggctgggtgc gaaggagccg gccagcatcg
     1861 ccgactggtc caggatgaaa taccgccgct tcatcgacgc catcggcggc tgggacgcct
     1921 tccagcggat cctcgccgcc gccggggcca ttgccgaaaa gcatggcgtg aagatcgcca
     1981 atgtggcgac ccgctgggtg ctcgaccagc cggctgtggc ggcggtcatc gtgggtgccc
     2041 ggcccggcga gcgcgaccac cgggaaacca accttcgcag cttcgccttc gccctcgacg
     2101 ccgaggacca cgcccgcttg gacgccgcct tcgccgccag ccgccctgtt cccggcgatt
     2161 gcggcgacga gtaccgccgc ccgcccttcc tcaccgcctc gggcgacctc agccaccatc
     2221 tcggcaccct cccccgggtc ttcgaagcga tacccgacgg cgaccgcagc cgggtctcga
     2281 gcggcagcgt ctgggaggag gtcgcgggct acacgagggc gctccgcgtc ggcgaccggg
     2341 tgctggtgag cggcacgacg gcgacgcacg ggaccgacca cgtggtggcc aggggcgacc
     2401 cggcggcgca gaccgcctat gtcctggaca agatccaggc gagcctccgg gccgtcggcg
     2461 ccgggctcga ggacgtcgtc cgcacccgcg tcttcgtgcg cgacatcgcc cagtgggagc
     2521 ccgtggcccg cacgcacggc cgctacttcc gcgaccaccg tccggtcaac tcgctggtcg
     2581 aggcgaagct ggtcggcgac tacctggtcg agatcgaggc cgaggcggtg gtccgtcaag
     2641 cctgagccgg caggccgagc agcgatcgcg cctcggccac cgtcgccggc cgcctgccat
     2701 actccgggca gagcgccacc acctgctcga ccagcgccgc gttggagggc gccaaccgct
     2761 ccttgtcgag gcgcacattg tcctcgagcc cggtccggca atgcccgccc tgctcgagcg
     2821 accagcgcac cagcgtgatc tggtccctgc cgatcccggc cccggtccag gtggcgtcgg
     2881 gtgccagccg cttcaccgtc cgcacgtaga aatcgaagac ctcgcgatcg accggcatgg
     2941 cgttcttcac gcccatcacg aactggacgt ggagcggccc cctgatctgc cccgccttct
     3001 ccatctcgac cgccttgaag agcatcgaga gatcgaagac ctcgatctcc ggcttcaccc
     3061 cgtgctcgag catcagggcc gccagccagt cgaccagatc agggctgttc tcgtagatcc
     3121 gggtcgggaa gttgaccgag ccggtcgaga gcgacgccat gtcgggcttg agcggcagca
     3181 tcccgccccg ctcctcgccc cgccccgacc ggccaccggt cgagaactgg acgatcatgc
     3241 cggggcaatg cttgcgcaga ccttcgagca gccggccgaa ccgctcgggg tccgaggtcg
     3301 gcttgccctc gtcgtcccgc acatggcaat gcgccagcgt ggcccccgcc tcgaacgccg
     3361 cctgggtcga ttcgacctgc tccgcgacgc tgatcggcac ggccgggttg ttggccttgg
     3421 tcggcagcga gccggtaatg gcgacggtga tgatgcaggg ctcggccatg cggcaaacct
     3481 cgaagctcgt gttccaaagg gcagaggcgc acagaacacc gttccaaaac aataggttaa
     3541 ctgaactccg cctgcgggct aaccaaggcc cataaccaaa tcgtaagctc caacacccgg
     3601 aacggcaagg tagcgtggcc aatcggttga gctgtccact taaccctctt taccagcctt
     3661 ttagctccgt ctagaggccc taggctctga acccgattag catattgaat agttgttatt
     3721 tttatgattc ttcgtgctca agagaacgct aaggaggtcg agaatgacga gctatccctt
     3781 ctggctgaac ga
//
LOCUS       DAOTFI010000346         3786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_28648_length_3786_cov_7.395336, whole genome shotgun sequence.
ACCESSION   DAOTFI010000346 DAOTFI010000000
VERSION     DAOTFI010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28648_length_3786_cov_7.395336"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="ugpB"
                     /locus_tag="P5558_22720"
     CDS             complement(<1..1083)
                     /gene="ugpB"
                     /locus_tag="P5558_22720"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HRY27196.1"
                     /translation="MRTRNLLAVMAAMALAGTSTTAMAATQIQWWHAMGGANGERVEK
                     IAADFNASQDAYEVIPTFKGSYAETMTAAIAAFRAQEQPHIVQVFEVGTATMMGAKGA
                     IYPVYQLMADAGEPFDPDAFLPAVAGYYTTTDGEMLSMPFNSSTPVFWYNKDAFEKAG
                     LDPEAPPATWPEFEEAAKALQAAGYPCGFTTAWESWIHVENFSAWHNLPIGTLSNGFG
                     GLDTELVVNQTPVVDHIAKMGEWQKSKIFDYGGRTNQAAPKFYNQECAVYTESSAGYA
                     GIKANVKDFEFGIGMLPYWPDIEGAPQNSIIGGASLWVLQGHDAAEYEGVAKFFTYLS
                     SPEVQAWYHQETGYLPITTAAYELSQE"
     gene            complement(1213..1644)
                     /locus_tag="P5558_22725"
     CDS             complement(1213..1644)
                     /locus_tag="P5558_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRY27197.1"
                     /translation="MRSYAEAMRRLKDSGLRPTRQRLSLLGMLIETAPRHVTAEELFQ
                     EARQAGIPVSLATVYNTLNQFTQAGLMGEVVVGSGQSYFDTDPSSHVHYFDKSTGEIY
                     DIPRDVVTITDLPEPPPGKVIDRVDVVVRIKNVCKADAPGD"
     gene            complement(1730..3136)
                     /locus_tag="P5558_22730"
     CDS             complement(1730..3136)
                     /locus_tag="P5558_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRY27198.1"
                     /translation="MSFVSPLVVAGHEVWPLVEGGKGVAVSNGRSAGAWAAAGGVGTF
                     SAVNADVLDANGDYVPLVFKGRDRRERHEELIDYSIRGAVCQARVAHETQGGEGRLHM
                     NVLWEMGGCERVLHGVLQQAKGLVHGVTCGAGMPYRLGEICASYGVHYYPIISSARAF
                     NALWKRAYGKVADWLGAVVYEDPWRAGGHNGLSNSEDPTRPEDPYPRVLELRKLMRGM
                     GLGDVPIIMAGGVWHLKDWKSWIGNKELGPIAFQFGTRPLLTQESPIPNGWKKRLLGL
                     LPDDVLLHRFSPTGFYSSAVKNRFLEELVERSDRQVAISDEADPASERTAFFAYGRGG
                     RGVYVTEADLLRIQGWLSQGYSQPMRTPDSTLIFVTPEGARQIRRDQADCMGCLSHCA
                     FSNWKDRDDFSTHRPVDPRSFCIQKTLQDIAHGRGEHEDQLMFAGHNVYKFREDPFYA
                     NGFVPTVRQLVQRILTGH"
     gene            complement(3178..>3786)
                     /locus_tag="P5558_22735"
     CDS             complement(3178..>3786)
                     /locus_tag="P5558_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRY27199.1"
                     /translation="RIHILGEMAGCMGMPRPELRDSVPRITTLNIPTDKIGAVIGPGG
                     KMIREICESTGAKIDINDEGLVKVASSDAMAAQRAVDWIRGLTASPEIGHVYTGKVIK
                     IVDFGAFISFLGSKDGLCHISELANERVASVGDVVKEGDTVKVQVLAVDDRGKIKLSM
                     RSVDQETGDVIEVERKPRPEREGGERGGRGERDGGRRRERAG"
BASE COUNT          570 a         1283 c         1269 g          664 t
ORIGIN      
        1 ctcctggctc agctcgtagg ccgccgtggt gatcggcagg tagcccgtct cctggtggta
       61 ccaggcctgg acctcgggcg aggagaggta ggtgaagaac ttcgccacgc cctcgtactc
      121 tgccgcgtca tggccctgca gcacccagag cgaggcgccc ccgatgatcg agttctgcgg
      181 ggctccctcg atgtccggcc agtagggcag catgccgatg ccgaactcga aatccttgac
      241 gttggccttg atgcccgcgt agccggccga gctctcggtg tagacggcgc attcctggtt
      301 gtagaacttg ggtgcggcct ggttggtgcg gccgccatag tcgaagatct tcgacttctg
      361 ccactcgccc atcttggcga tatgatcgac caccggcgtc tggttgacca cgagctcggt
      421 atccagcccg ccgaagccgt tcgagagcgt gccgatcggc aggttgtgcc aggccgagaa
      481 attctcgacg tggatccagc tctcccaggc cgtcgtgaag ccgcagggat agccggcggc
      541 ctgcagcgcc ttggcggcct cctcgaactc gggccaggtg gcgggcggcg cttccggatc
      601 cagacccgcc ttctcgaagg cgtccttgtt gtaccagaag actggcgtcg agctgttgaa
      661 cggcatcgac agcatctcgc cgtcggtggt cgtgtagtag ccggcgacgg ccggcaggaa
      721 ggcgtccgga tcgaatggct cgccggcatc ggccatgagc tggtagaccg ggtagatggc
      781 gcccttggcg cccatcatgg tcgcggtgcc gacctcgaac acctggacga tgtgcggctg
      841 ctcctgcgcc cggaaggcgg cgatcgcggc cgtcatggtc tcggcatacg agcccttgaa
      901 ggtcgggatg acctcgtacg cgtcctggct ggcgttgaaa tcggcggcga tcttctcgac
      961 ccgctcgcca ttggccccgc ccatggcatg ccaccactgg atctgcgtcg ccgccatcgc
     1021 cgtggtgctc gtgccggcca gcgccatggc ggccatcact gcaagcaagt tcctggttcg
     1081 catcgttccg ctccttctcc ctgtcggggg atcatgcacc gggcgctttc gaccatgcaa
     1141 gcgtgatggt caaatgacca gggcaggcag gcggaaacag gacaggcccg agggacgggc
     1201 cggagcggtg gctcagtcgc cgggggcgtc ggccttgcac acgttcttga tccgcaccac
     1261 gacgtcgacc cggtcgatga ccttgcccgg cggcggctcg ggcaggtcgg tgatcgtcac
     1321 cacgtcgcgc ggaatgtcgt agatctcgcc cgtcgacttg tcgaaataat ggacatgcga
     1381 gctggggtcg gtgtcgaaat aggactggcc cgagccgacc acgacctcgc ccatgaggcc
     1441 cgcctgggtg aactggttga gcgtgttgta gaccgtcgcg agcgaaaccg ggatccctgc
     1501 ctgccgggcc tcctggaaca gctcttcggc ggtcacgtgc cgaggggcgg tctcgatcag
     1561 catgccgagc agggacaggc gctgccgggt cggccgcaag cccgagtcct tcaaacgccg
     1621 catcgcttcc gcgtaagatc tcatcggtcg aaacccacgt cctctggcgc ttcaattccc
     1681 ataccggact tatctggtcc gttatgcgtg gccggtcaag cggccggcat cagtggccag
     1741 tcaggatgcg ctgcaccaat tgccgcacgg tcggcacgaa gccgttggcg tagaaggggt
     1801 cttcccggaa cttgtagacg ttgtgcccgg cgaacatgag ctggtcctcg tgctcgccgc
     1861 gaccatgcgc gatgtcctgc agggtcttct ggatgcagaa ggagcgcggg tccaccgggc
     1921 ggtgggtgga gaagtcgtcc cggtccttcc agttgctgaa ggcgcagtgg ctgaggcagc
     1981 ccatgcagtc ggcctggtcg cgccggatct ggcgggcacc ctcgggcgtc acgaagatca
     2041 gcgtgctgtc cggcgtgcgc atcggctggc tgtagccctg ggacagccag ccctggatgc
     2101 gcagcagatc ggcctcggtc acatagacgc cgcgcccgcc ccggccatag gcgaagaacg
     2161 ccgtgcgctc gctcgccgga tcggcctcgt cgctgatcgc cacctggcga tcggagcgct
     2221 cgaccagctc ctcgaggaag cggttcttca ccgccgagga atagaacccg gtcgggctga
     2281 agcggtgcag caggacgtcg tcgggcagga ggccgagcag ccgcttcttc cagccgttcg
     2341 ggatcgggct ctcctgggtc aggagcggcc gcgtgccgaa ctggaaggcg atgggaccga
     2401 gctccttgtt gccgatccag ctcttccagt ccttcaggtg ccagacgccg cccgccatga
     2461 tgatcggcac gtcgccgaga cccatgccgc gcatcagctt gcggagctcg agcacgcgcg
     2521 ggtaggggtc ctcggggcgc gtcgggtcct cgctgttgga gagaccgttg tggccgccgg
     2581 cgcgccacgg gtcctcgtag accacggcac cgagccagtc ggcgaccttg ccgtaggcgc
     2641 gcttccagag cgcgttgaag gcccgcgccg aggagatgat cgggtagtag tggacgccgt
     2701 agctggcgca gatctcgccc agccggtagg gcatgccggc gccgcaggtg acgccgtgca
     2761 ccagcccctt ggcctgctgc agcacgccgt gcagcacgcg ctcgcagccg cccatctccc
     2821 agagcacgtt catgtgcagg cggccttcgc cgccctgggt ctcgtgcgcc acgcgcgcct
     2881 ggcagaccgc accgcggatc gagtagtcga tcagctcctc gtgccgctcg cggcggtcgc
     2941 ggcccttgaa gaccagcggc acgtagtcgc cattggcgtc gagcacgtcg gcgttgaccg
     3001 ccgagaacgt gccgacgccg ccggccgccg cccaggcgcc cgcgctgcgg ccattgctga
     3061 ccgccacacc cttgccacct tcgaccagtg gccagacttc atgtccggcc accacgagcg
     3121 gactgacgaa gctcatgccg gtctccctgc tgcttgtgcc ggcgcggggc gggccgctca
     3181 gccggcccgc tcgcgacggc gaccgccatc gcgctcgccg cggccaccac gctcgccacc
     3241 ttcccgttcc gggcgcggct tgcgctcgac ctcgatcacg tcgcccgtct cctgatcgac
     3301 cgagcgcatg ctgagcttga tcttgccacg atcgtcgacc gccagcacct gaaccttcac
     3361 cgtatcgcct tccttgacca cgtcgccgac gctggcgacg cgctcgttgg cgagctcgga
     3421 gatgtggcag aggccgtcct tgctgccgag gaaggagatg aaggcgccga aatcgacgat
     3481 cttgatcacc ttgccggtat agacgtgacc gatctccggg ctcgccgtca ggccgcggat
     3541 ccagtccacc gcccgctgcg ccgccatggc gtcgctggag gcgaccttga ctaggccctc
     3601 gtcgttgatg tcgatcttgg cgccggtgct ctcgcagatc tcgcggatca tcttgccgcc
     3661 cgggccgatc acggcaccga tcttgtcggt cgggatgttg agcgtcgtga tccgtggcac
     3721 ggaatcgcgc agctccggcc gcggcatgcc catgcagccg gccatctcgc cgaggatgtg
     3781 gatccg
//
LOCUS       DAOTFI010000347         3775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_28758_length_3775_cov_8.510215, whole genome shotgun sequence.
ACCESSION   DAOTFI010000347 DAOTFI010000000
VERSION     DAOTFI010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28758_length_3775_cov_8.510215"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /gene="fliF"
                     /locus_tag="P5558_22740"
     CDS             <1..1749
                     /gene="fliF"
                     /locus_tag="P5558_22740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRY27200.1"
                     /translation="MAEQVEALPVGVADAAVAASGARGGLAALLGALGAERIVAITVA
                     ILALVGLLGFLGMRALESPYTLLYAGLALEDTRTIVTRLQAMDVPHRLSPAGDAVLVP
                     ADRVLDLRMALATDGLPGGGVVGYEIFDAVDAFGTTEFLSNINLKRAMEGELARTIAS
                     LRQVRSARVHLVQPPRELFRREQAPASASVFLSVGPAGLGRSEIAAIQHLVASAVPGL
                     EPGRIAIADDTGRLLSRGGDALDTAIGLDDTDGYRQAFESRLQAKILQLLERSLGVGR
                     VEAQVTAELDFDSVSTTEEIFDPERQVVRSTQLVDESTELDESTPNDAVSVANNLPGG
                     DAQGAAATNREQRNRNEETINYEISRTVRNQTRHGGRLQRLSVAVQVDGTYRDGADGV
                     PVFEPLPAADLAELETLVRGAVGIDDERGDTLSIISRRLARPEPLPVVDDGTLLGFAH
                     ADLMRFAELGGLAGIALLVLLLGVRPLLRRLQPARTGPGNAGGVRLLTGPDGRQLLIQ
                     ATPGTAVTIDERGNPVVTRNGQPGDAAGEQTGDRVSLRQIEGDVRESLLTDMAELIED
                     RPEDAIRVIRSWLHSS"
     gene            1762..2781
                     /gene="fliG"
                     /locus_tag="P5558_22745"
     CDS             1762..2781
                     /gene="fliG"
                     /locus_tag="P5558_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRY27201.1"
                     /translation="MPSSDFMQFDKLTGVEKAAILMLAVSEENAARLFARLELDEVKE
                     LSQTMSLLGRVGSDVVEKIIGDFAERLSQAGGVVGSFDTTERLLQRFMDTERVEQIME
                     EIRGPAGRTVWDKLGNVNESVLASYLKNEYPQTVAVVLSRIEAAHAARVLASLPDDFS
                     VEVVMRMLRMEVVQKDILQDVERTLRAEFMSNLARTNRRDNHEVMAEIFNYFDRNTET
                     RFVAALEERNKEAADRIKALMFTFEDLVKLDSSGIQTLMRNAGNDRIGIALKGASEQL
                     KELFFANMSERAAKILKEDMQAMGPVRLRDVDEAQQFLVNMAKELAASGEIFLADSKD
                     DELVY"
     gene            2781..3518
                     /locus_tag="P5558_22750"
     CDS             2781..3518
                     /locus_tag="P5558_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27202.1"
                     /translation="MAEPVRAFAFTRKHRPAAAAGGTPAWPVIAFGTAPADAAKAPDG
                     AGQGLVPVPELVFDEADLARICAAVDEAARQAAREEMARAIEGRTMAALECLASAIDA
                     LDAALDARVAELRRTAANLAAATVEALGASSSERVAVRLAETLVDECLGRLDPLLPLT
                     IEVAAELVDPLAAALGRVPGLPARPGRLAIEPARDLAPGEARLVWPDGHAEWSLTELR
                     HRAALILDGLAGDGAPAIAEPNDTREG"
     gene            3591..>3775
                     /locus_tag="P5558_22755"
     CDS             3591..>3775
                     /locus_tag="P5558_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HRY27203.1"
                     /translation="MPVPLASGDRLSAIYDIPVQLAAVLGKTSMPVNQLLRLGRGAVV
                     ELDRKVGEPIDIYVNNRL"
BASE COUNT          566 a         1373 c         1313 g          523 t
ORIGIN      
        1 atggctgagc aagtcgaggc actgccggtg ggggtcgccg atgcggcggt cgccgcaagc
       61 ggtgcgcgag gcgggctggc ggccctgctg ggtgccctgg gcgccgagcg gatcgtcgcc
      121 atcaccgtcg ccatcctcgc cctcgtcggg ctgctcggct ttctcggcat gcgggcgctc
      181 gaatccccct acaccctgct ctatgcgggc ctcgccctgg aggacacccg caccatcgtc
      241 acccgcctgc aggccatgga cgtgccgcac cggctgtcgc cggccggcga tgccgtgctg
      301 gtgcccgccg accgggtgct cgatctgcgg atggcgctcg ccacggacgg cctgccgggc
      361 ggcggggtcg tcggctacga gatcttcgat gccgtcgatg cgttcggcac gaccgagttc
      421 ctctccaaca tcaacctcaa gcgcgccatg gaaggcgagc tcgcccgaac catcgcgagc
      481 ctgcgccagg tgcgctccgc ccgggtccat ctcgtccagc cgccgcgcga gctcttccgt
      541 cgcgagcagg cgccggccag cgcctcggtc ttcctctcgg tcgggcccgc gggcctcggt
      601 cgcagcgaga tcgccgccat ccagcatctc gtcgcctcag ccgtgcccgg gctggagccc
      661 ggccgcatcg ccatcgccga cgacaccggc cggctcctgt cgcggggcgg cgacgccctg
      721 gacaccgcca tcggcctcga cgacaccgac ggctaccgcc aggccttcga gagccgcctg
      781 caggcgaaga tcctgcagct gctcgagcgg agcctcggcg tcggccgggt cgaggcgcag
      841 gtcacggccg agctcgactt cgacagcgtc tcgaccaccg aggagatctt cgaccccgag
      901 cggcaggtgg tgcgcagcac ccagctcgtc gacgagtcga cggagctgga cgagagcacg
      961 cccaacgacg ccgtcagcgt cgccaacaac ctgccgggcg gtgacgccca gggtgcggcc
     1021 gccacgaatc gcgagcagcg caaccgcaac gaagagacga tcaactacga gatctcgcgc
     1081 accgtgcgca accagacccg ccacggcggc cggctgcagc gcctgtcggt cgccgtgcag
     1141 gtcgacggca cctatcgcga cggcgccgat ggcgtgcccg tcttcgagcc gctgccggcg
     1201 gccgacctcg ccgagctcga gacgctggtg cgtggtgctg tcggcatcga cgacgagcgc
     1261 ggcgacaccc tctcgatcat cagccgtcgg ctggcccggc ccgagccgct gccggtggtc
     1321 gacgacggca cgctgctcgg cttcgcccat gccgacctga tgcgattcgc cgagctcggc
     1381 gggctcgccg gcatcgccct gctcgtcctc cttctcggcg tgcggccgct cctgcgccgg
     1441 ctgcagccgg cccggaccgg cccgggcaat gccggcggcg ttcgcctgct gaccggcccc
     1501 gacggccggc agctcctgat ccaggccacg cccggcacgg ccgtcaccat cgacgagcgc
     1561 ggcaacccgg tcgtgacccg caacggccag ccgggcgacg ccgcaggcga gcagaccggg
     1621 gaccgggtca gcctgcgcca gatcgagggc gacgtccgcg aatcgctgct gaccgacatg
     1681 gccgagctga tcgaggaccg tcccgaggac gcgatccgcg tgatccgctc ctggctgcat
     1741 tcgagctgag gtcgacctgc catgccatca tccgacttca tgcagttcga caagctcacc
     1801 ggcgtcgaga aggcggccat cctgatgctc gccgtcagcg aggagaacgc cgccaggctg
     1861 ttcgcccggc tggagctcga cgaggtcaag gagctgtcgc agacgatgtc gctgctcggc
     1921 cgggtcggct ccgacgtggt cgagaagatc atcggcgatt tcgccgagcg gctgagccag
     1981 gccggcggcg tggtcggcag cttcgacacc accgagcggc tcctgcagcg cttcatggat
     2041 acggagcggg tcgagcagat catggaggag atccgcggcc ccgccggccg cacggtctgg
     2101 gacaagctcg gcaacgtcaa cgagtcggtg ctcgcctcgt acctcaagaa cgaatacccg
     2161 cagaccgtgg ccgtcgtgct gtcgcgcatc gaggccgccc atgcggcccg tgtcctggcc
     2221 agcctgccgg acgatttctc cgtcgaggtg gtgatgcgca tgctgcgcat ggaggtcgtg
     2281 cagaaggaca tcctgcagga cgtcgagcgc acgctgcgcg ccgagttcat gagcaacctg
     2341 gcgcgcacca accggcgcga caatcacgag gtgatggccg agatcttcaa ctacttcgat
     2401 cgcaatacgg aaacgcgttt cgtcgccgcc ctcgaggagc gcaacaagga ggcggccgac
     2461 cggatcaagg ccctgatgtt caccttcgag gacctggtca agctcgacag ctcgggcatc
     2521 cagaccctga tgcgcaatgc cggcaacgac cgcatcggca tcgctctcaa gggggcgagc
     2581 gagcagctca aggagctctt cttcgccaac atgtccgagc gcgccgcgaa gatcctcaag
     2641 gaggacatgc aggcgatggg gccggtgcgc ctgcgcgacg tcgacgaggc ccagcagttc
     2701 ctcgtcaaca tggcgaagga gctggccgcc tccggcgaga tcttcctcgc cgacagcaaa
     2761 gacgacgagc tggtctattg atggcggaac ccgtgcgcgc cttcgcgttc accaggaagc
     2821 accggccggc cgcggcggcc ggtggcacgc ccgcctggcc ggtcatcgcc tttgggacgg
     2881 ccccggcgga cgcggcgaag gcgccggatg gagcgggcca gggtctcgtc ccggtgcccg
     2941 agctggtctt cgacgaggcc gatctcgccc gcatctgcgc cgccgtcgat gaggcggccc
     3001 gccaggcggc acgcgaggag atggcgaggg ccatcgaggg gcgcacgatg gccgcgctcg
     3061 agtgcctggc gtccgccatc gacgcgctcg atgcggcgct cgacgcccgc gtcgccgagc
     3121 tgcgccggac ggcggcgaat ctggcggccg ccacggtgga agcgctgggt gcctcctcgt
     3181 ccgagcgcgt cgccgtccgg ctcgccgaaa cgctggtcga cgaatgcctc ggccggctcg
     3241 acccgctgct gccgctgacc atcgaggtgg cagccgagct ggtcgatccg ctggccgcag
     3301 ccctcggacg ggtgcccggg ctcccggccc ggcccggccg gctggccatc gagccggccc
     3361 gggacctggc gccgggcgag gcgcggctgg tctggccgga cggccatgcc gaatggtcgc
     3421 tgaccgagct ccggcatcgg gcggccctga tcctcgacgg cctcgctggc gatggcgccc
     3481 ctgccatcgc cgaaccgaac gacacaaggg aagggtgagc catgaccacc gaccatcgag
     3541 ccgtcgggca ggcggcggat gccgccgacg caacggaagc gctggacggg atgccggtgc
     3601 cgctggcctc cggcgaccgg ttgagcgcca tctacgacat tccggtccag ctcgccgcgg
     3661 tgctcggcaa gacctcgatg ccggtcaacc agctcctgcg gctcggccgc ggtgcggtgg
     3721 tcgagctcga ccgcaaggtg ggcgagccga tcgacatcta cgtcaacaac cgcct
//
LOCUS       DAOTFI010000348         3733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_29195_length_3733_cov_6.389342, whole genome shotgun sequence.
ACCESSION   DAOTFI010000348 DAOTFI010000000
VERSION     DAOTFI010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29195_length_3733_cov_6.389342"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="P5558_22760"
     CDS             complement(<1..825)
                     /locus_tag="P5558_22760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRY27204.1"
                     /translation="MPALTDLLAEGEPAPNHRPWPRTLIEPARWSFAIAQLAEGNWTL
                     MGLWGEPETAHMALLDDETGDLGVVSLDCPDRRYPSVGRFHPPAIRLERALRDLFGLE
                     PDGLPDTRPWLDHGKWGVSRPLGDARPTTGEPVPYPFLAAEGEALHEVPVGPVHAGII
                     EPGHFRFTASGETVVRLEERLGYVHKGVESLMAGADLARGAKLAGRLSGDATVAWASA
                     FARAAEAATCREAPPRAQALRALMAELERLANHLGDIGAVCNDASFALMLAECGILR"
     gene            complement(851..>1345)
                     /locus_tag="P5558_22765"
     CDS             complement(851..>1345)
                     /locus_tag="P5558_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY27205.1"
                     /translation="AFGMGGPLANFAGLLHMVMHSLTKSAIFFAVGNIAQVKGTQRIA
                     EIRGLTQSHPALGWGFVIGVLAIAGLPPMGIFMSEFLVVSSTFARAPLLAIPLVLGLL
                     LGLGALLLRLTGIAFGEPTGSTAPATGTNLPMQAHLALVLTAGVYLPPPLVAWFQNVG
                     VLLG"
     assembly_gap    1346..1445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1446..2089)
                     /locus_tag="P5558_22770"
     CDS             complement(<1446..2089)
                     /locus_tag="P5558_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY27206.1"
                     /translation="MSQGPIAAVLVIPLLAAALLAILPGYRLTARLNVAASLLTLLAA
                     LLLLMGERPAPGAYLLVDDLNIVFIVLNCFVAFTTSVFSAGYIQHELETGRLTATRLR
                     FYHAMYQVMMFGMNLAFVSSNIGLMWVAVELATLTTVLMVGIYQTHEALEAAWKYFIL
                     GSVGIALALFGTILFYTAAQPVVGEGADAMIWTVLLSRAAELDPALLSVAFVFLL"
     gene            complement(2086..2748)
                     /locus_tag="P5558_22775"
     CDS             complement(2086..2748)
                     /locus_tag="P5558_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-4 component E"
                     /protein_id="HRY27207.1"
                     /translation="MGSLAFDIAHLLAGGLVLVSFMLLYQDRLSALVNVFALHALVLA
                     LSVAWQAFVQEAPHLYVTALIAVAFKVLVVPLALHRIIRRLGIHREIETVVGIGPTML
                     AGMGLVALSMAVILKITPEADPLAREDLAFALAVLLLGLLLMVTRRNAVSQVIGFMSL
                     ENGLILAATGARGMPLVVEISVAFSILIAFIVIGVFLFRIRERFDTVDTSALDEFRGE
                     RR"
     gene            complement(2762..3733)
                     /locus_tag="P5558_22780"
     CDS             complement(2762..3733)
                     /locus_tag="P5558_22780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRY27208.1"
                     /translation="MELIGVELIAGLAVQGAQMALVLLLAPGLTGLVRTLKARLLRRR
                     GPPVLQPYRDLARLLRKEVVLAENASWLFRAAPYLIFALTWVAAALVPTFASGLLFSW
                     TADLIVIVALLASARFFLALAGMDVGTGFGGIGASREMMISSLAEPAMLLIIFTIALV
                     AGSTRLATITAYLASDEVGLRVSLGLALIALVIVAIAENARIPVDNPATHLELTMVHE
                     AMVLEYSGRHLAMIELAASLKLLLFVALIASVFLPFGLADAGAGPLAWLQGLALFAAK
                     LVAAAALLALFETSIAKMRVFRVPQFLGAALMLGLLGTLLLFVSRSL"
BASE COUNT          654 a         1096 c         1450 g          433 t
ORIGIN      
        1 gcgcaggatg ccgcattcgg ccagcatcag cgcgaaggag gcgtcgttgc agaccgcacc
       61 gatgtcgccg aggtggttgg cgagccgttc cagctcggcc atcagggcgc ggagcgcctg
      121 cgcgcggggc ggcgcctcgc ggcaggtcgc ggcttcggcg gcgcgggcga aggcgctggc
      181 ccaggcgacg gtggcgtcgc ccgagagccg gccggcgagc ttcgcacccc gggcgaggtc
      241 ggccccggcc atgaggctct cgaccccttt gtggacatag cccagccgct cctcgaggcg
      301 gaccacggtc tcgcccgagg cggtgaagcg aaaatggccg ggctcgatga tcccggcatg
      361 gacggggccg accggcacct cgtgcagcgc ctcgccctcg gcggcgagga aggggtaggg
      421 cacgggctcg ccggtcgtgg ggcgcgcatc gccgagcggg cggctgacgc cccatttgcc
      481 atgatccagc cagggccgcg tgtccggcag gccgtcgggc tcgaggccga agagatcgcg
      541 cagcgcccgc tcgaggcgga tggccggcgg gtggaagcgg ccgaccgaag ggtagcggcg
      601 gtcgggacag tcgaggctga ccacgccgag atcgcccgtc tcgtcgtcga ggagcgccat
      661 gtgggcggtt tcgggctcgc cccagaggcc catgagggtc cagttgccct cggcgagctg
      721 ggcgatggcg aagctccagc gggcgggctc gatgagggtg cggggccagg ggcgatggtt
      781 cggtgccggc tcgccctcgg ccaaaaggtc ggtcagggcc ggcatggggc atccctcatt
      841 tgatctggcc tcatccgagc agcaccccga cgttctggaa ccaggcgacg agcggcggcg
      901 gcaggtagac gccggccgtc agcacgagcg ccaggtgcgc ctgcatcggc aggttggtgc
      961 cggtggcggg ggcggtgctg ccggtgggtt cgccgaaggc gatgccggtg aggcggagca
     1021 ggagcgcacc caggcccagg agcaggccga ggacaagggg gatggcgagg agcggtgccc
     1081 tggcgaaggt ggagctgacg accaggaact cgctcatgaa gatgcccatg ggcggcaggc
     1141 cggcgatggc gagcacgccg atgacgaagc cccagccgag ggccgggtgg ctctgggtca
     1201 ggcccctgat ctcggcgatg cgctgggtgc ccttgacctg ggcgatgttg ccgacggcga
     1261 agaagatcgc ggacttggtc aggctgtgca tgaccatgtg caggaggccg gcgaagttgg
     1321 cgagcgggcc gcccatgccg aaggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnagcag gaagacgaag gcgacgctca agagcgccgg gtcgagctcg gcggcgcgcg
     1501 aaagcagcac ggtccagatc atggcatccg cgccctcgcc gacgacgggc tgggcggcgg
     1561 tgtagaagag gatcgtgccg aagagcgcca gggcgatgcc gacgctgccg aggatgaagt
     1621 atttccaggc ggcctccagg gcctcgtgcg tctggtagat gccgaccatg aggacggtgg
     1681 tgagcgtcgc cagctccacg gcgacccaca tcagcccgat attgctcgag acgaacgcca
     1741 ggttcatgcc gaacatcatc acctggtaca tggcatggta gaagcggagc ctggtcgccg
     1801 tcagccggcc ggtctcgagc tcgtgctgga tgtagccggc gctgaagacg ctggtggtga
     1861 aggcgacgaa gcagttgagc acgatgaaga cgatgttgag gtcgtcgacg aggaggtagg
     1921 cgccgggggc ggggcgctcg cccatgagca gcaggagcgc tgccagcagc gtcagcaggc
     1981 tcgccgccac gttcagccgg gcggtcagcc ggtagccggg caggatcgcg agcagggccg
     2041 ccgcaagcag cgggatcacg aggacggcgg cgatcgggcc ctggctcacc ggcgctcgcc
     2101 ccggaactcg tcgagggcgc tggtgtcgac cgtgtcgaag cgctcgcgga tgcggaagag
     2161 gaagacgccg atgacgatga acgcgatgag gatcgagaag gcgacgctga tctcgaccac
     2221 gagcggcatg ccgcgcgccc cggtggcggc caggataagg ccgttctcga gcgacatgaa
     2281 gccgatcacc tgactcaccg cgttgcggcg ggtgaccatc aggaggaggc ccaggagcag
     2341 cacggcgagg gcgaaggcga gatcctcgcg ggcgagcggg tcggcctccg gcgtgatctt
     2401 caggatgacc gccatggaga gggcgacgag ccccatgccg gccagcatgg tcgggccgat
     2461 gccgaccacg gtctcgatct cgcgatggat gccgagccgg cggatgatgc gatggagggc
     2521 gagcggcacc accagcacct tgaaggcaac ggcgatgagg gcggtgacgt agagatgcgg
     2581 tgcctcctgg acgaaggcct gccaggcaac cgagagggca agcaccaggg catgcagcgc
     2641 gaagacgttg acgagggccg agagccggtc ctggtagagc agcatgaagc tgaccagcac
     2701 gagcccgccg gccaggagat gggcgatgtc gaaggcgagg ctgcccatca gaggctcccc
     2761 atcacagact gcgggagacg aagagcagga gcgtgccgag caggcccagc atgagggcgg
     2821 cacccaggaa ctgcggcacg cggaagaccc gcatcttggc gatcgatgtc tcgaagaggg
     2881 cgaggagggc cgcggccgcc acgagcttgg cggcgaagag ggcaaggccc tggagccagg
     2941 cgaggggacc ggcgccggcg tcggcgaggc cgaagggcag gaagacgctg gcgatgaggg
     3001 cgacgaacag cagcagcttc agcgaggcag cgagctcgat catcgcgagg tgccggccgg
     3061 aatattcgag caccatggcc tcgtggacca tggtgagctc gaggtgggtg gccgggttgt
     3121 cgacggggat gcgggcattc tcggcgatgg cgacgatgac cagcgcgatg agggcgaggc
     3181 cgagcgagac gcggagcccg acctcgtctg aggcgaggta ggcggtgatc gtggcgagcc
     3241 gggtcgagcc cgcgaccagg gcgatggtga agatgatgag cagcatggcg ggctcggcca
     3301 aggacgagat catcatctcg cggctggcgc cgatgccgcc gaagccggtg ccgacatcca
     3361 tgccggcgag ggcgaggaag aagcgcgcac tggcgagcaa ggccacgatg acgatgaggt
     3421 cggcggtcca gctgaagagg aggccggacg cgaaggtcgg cacgagggcc gccgccaccc
     3481 aggtcagggc gaagatcaga tagggggcgg cgcgaaagag ccaggaggcg ttctccgcca
     3541 gcaccacctc cttgcgcagg aggcgggcga ggtcgcgata gggctggagg acgggaggac
     3601 cccggcgtcg cagcagcctc gccttcaggg tccgcacgag gccggtgagc ccgggggcga
     3661 gcagcagcac gagcgccatc tgggcaccct ggacggcgag gccggcgatc agctccaccc
     3721 cgatcagctc cac
//
LOCUS       DAOTFI010000349         3727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_29271_length_3727_cov_5.319444, whole genome shotgun sequence.
ACCESSION   DAOTFI010000349 DAOTFI010000000
VERSION     DAOTFI010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29271_length_3727_cov_5.319444"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="P5558_22785"
     CDS             <1..782
                     /locus_tag="P5558_22785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY27209.1"
                     /translation="LLRYRHGSSHATVVDVAPTVACEDPWRYRNKMEFSFGNRRWVER
                     TEEAGLRDDETRSDPAPTSWNDLFTAEDGDLVTIPDITNTYGLLTLLMLNKIRGGDLD
                     DIQPGLDAVAELLDNGAQIVTSSPELQTAFAQGSTNFAAYAADYAHTLRAAGMPVAFV
                     QPEEGSPAFFITANVVAGRPNTELAKAFVDFSLRAEAQAGWAEAMRYSPSNKEVVLDD
                     DMTAGMVYGQEAIDRVVRFDPEKVNAERAAWTDAWNRTIAR"
     gene            788..1633
                     /locus_tag="P5558_22790"
     CDS             788..1633
                     /locus_tag="P5558_22790"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27210.1"
                     /translation="MPGAKGGQGLVEPIVLVTPGIALLFGAFLFPVGQILVLSVVPSG
                     EELGAWSNFARFFADDYYLRVAWRTFRLSLLITAATALIGFPLAYAMARAPRWLAFLL
                     LLLTVLPLMTSVVVRTFGWMMVLGRGGPVTDLLMLFGLSARSAVLLQTETGIVVAMVQ
                     VLLPFMVLTTLGVIAGIHPHLEEAARTMGAGFYRTLRHVVLPLSLPGLVSGSLLVFAI
                     SMSSFITPNLIGGVRLPVMASAIYQQAIHTLDWGFAAAQSTLLLIAVLGLLVPYLALM
                     RRARV"
     gene            1635..2435
                     /locus_tag="P5558_22795"
     CDS             1635..2435
                     /locus_tag="P5558_22795"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27211.1"
                     /translation="MVGRFPPSALVLGGLALALILVFMLLPLGVVVAASLTAGQFLTF
                     PPQGLSFRWYGDVLASEAYLSSAWTSLKVATLVTIAATVIGTAAAIGLSRRRLPGRAL
                     LAGVFLSPLVLPSIIFGIGLLIVFSIWGNGPSLLALALGHTVLTMPYVVRTVAAVLAD
                     ADPAIEEAARTMGAGPVDRLRFVVLPQAKGGILAGAFFAFNVSFDEAVIALFLRTPGV
                     ETLPMRIYGQLEFSPTPAVAAVSTLMILMTILVVVAIERSLGLRKVAT"
     gene            2432..3481
                     /locus_tag="P5558_22800"
     CDS             2432..3481
                     /locus_tag="P5558_22800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY27212.1"
                     /translation="MTFLAIENVGKRFGHTTALDGVSLGIERGSFVALLGPSGCGKST
                     LLRIIAGLETPTFGRITVDGEDITRLPPERRRLAMMFQSYALLPHMTALENVRFPLRM
                     QGREGAAEQRRRAMETLALVGLDHLAERLPREMSGGQQQRVALARAIIAEPRVLLLDE
                     PLSNLDARLREDMQIELQALHRRLGLTTLFVTHDQEEALSLADRVVLLNQGRIDRDAT
                     ADALYDDPGTAFAADFLGAANLVPVTVEATEDGHTAILEDGQRLQLASAPGPGPRQLV
                     LRQEDITLADPSEPGLEATIEARIFLGTRVRYLVRLGDLRLRVLAPRHLQYPPGTPVR
                     LQIDPANCRWSREGP"
BASE COUNT          518 a         1483 c         1101 g          625 t
ORIGIN      
        1 tcttgttgcg gtaccgccac gggtcctcgc acgcgaccgt cgtcgacgtc gcgccgacgg
       61 tcgcgtgcga ggacccgtgg cggtaccgca acaagatgga gttctccttc ggcaaccggc
      121 gctgggtcga gcgcacggag gaggcgggcc tgcgcgacga cgagacccgg agcgaccccg
      181 cgcccacgag ctggaacgac ctcttcaccg cagaggacgg cgatctcgtc accatccccg
      241 acatcaccaa cacctacggc ctgctcacgc ttctgatgct gaacaagatc aggggcggcg
      301 acctcgacga catccagccc ggcctcgacg ccgtggccga gctgctcgac aatggcgcgc
      361 aaatcgtcac ctcctcgccc gagctgcaga ccgccttcgc ccagggctcc accaacttcg
      421 ccgcctacgc cgccgactac gcccacacgc tccgggccgc cggcatgccg gtcgcgttcg
      481 tccagccgga ggaaggctcg cccgccttct tcatcaccgc caacgtggtg gccggccggc
      541 ccaacaccga gctcgccaag gccttcgtcg acttctcgct ccgcgccgag gcgcaggccg
      601 gctgggccga ggccatgcgc tattcgccgt ccaacaagga ggtcgtgctc gacgacgaca
      661 tgacggccgg catggtctac ggccaggagg ccatcgaccg cgtcgtccgc ttcgatccgg
      721 agaaggtgaa tgccgagcgc gccgcctgga cggacgcctg gaaccgcacc atcgcccgct
      781 gagcccgatg ccgggtgcga aaggcgggca ggggctggtc gagcccatcg tcctcgtcac
      841 ccccggcatc gccctcctct tcggcgcctt cctcttcccg gtcggccaga tcctcgtcct
      901 gagcgtcgtg ccgtccgggg aggagctcgg cgcctggagc aatttcgccc ggttcttcgc
      961 ggacgactac tacctacgcg tcgcctggcg gaccttccgc ctctcgctgc tcatcacggc
     1021 ggcgaccgcc ctgatcggct tcccgctcgc ctacgccatg gccagggcac cccgctggct
     1081 cgccttcctc ctcctgctgc tcaccgtcct gccgctgatg accagcgtgg tggtgcgcac
     1141 cttcggctgg atgatggtgc tcggccgcgg cggcccggtc accgacctgc tcatgctctt
     1201 cggcctttcc gcccgcagcg ccgtgctgct gcagaccgag accggcatcg tcgtcgccat
     1261 ggtccaggtg ctgctgccct tcatggtgct gaccacgctc ggcgtcatcg ccggcatcca
     1321 cccgcatttg gaagaggcgg cccggaccat gggggccggg ttctaccgga cgctgcgcca
     1381 cgtcgtcctg ccgctcagcc tgcccggcct cgtctccggc tcgctcctcg tcttcgccat
     1441 ctcgatgagc tccttcatca cccccaacct gatcggcggc gtgcgcctgc cggtcatggc
     1501 gagtgcgatc taccagcagg ccatccacac cctcgactgg ggcttcgccg ccgcccagtc
     1561 gacgctcctg ctgatcgccg tgctcggcct cctcgtcccc tacctcgcgc tgatgcgccg
     1621 ggcacgggtc tgagatggtc ggccgctttc ccccgagcgc tctcgtcctc ggcggcctgg
     1681 cgctcgcctt gatcctggtc ttcatgctgc tgccgctcgg cgtcgtcgtc gccgcgtcgc
     1741 tcaccgccgg ccagttcctg accttcccgc cgcagggcct gtcgttccgc tggtacggcg
     1801 acgtgctggc gtccgaggcc tatctctcct ccgcctggac cagcctgaag gtggccaccc
     1861 tcgtcaccat cgccgccacg gtcatcggga ctgccgccgc cataggcctc agccgccgcc
     1921 ggctgccggg cagggcgctg ctcgccgggg tcttcctctc gcccctcgtc ctgccgtcca
     1981 tcatcttcgg catcggcctg ctcatcgtct tctcgatctg gggcaacggc ccctcgctcc
     2041 tggccctcgc cctcggccat accgtgctca ccatgcccta tgtcgtgcgc accgtggcgg
     2101 ccgtgctcgc cgacgccgat cccgcgatcg aggaggcggc ccggaccatg ggtgccgggc
     2161 ccgtcgaccg gctccgcttc gtcgtcctgc cgcaggccaa gggcggcatc ctcgccggcg
     2221 ccttcttcgc cttcaacgtc tccttcgacg aggcggtgat cgccctcttc ctgcgcaccc
     2281 ccggcgtcga gaccctgccg atgcgcatct acggccagct cgagttctcc ccgaccccgg
     2341 cggtcgccgc cgtctcgacc ctcatgatcc tgatgaccat cctcgtcgtc gtcgccatcg
     2401 agcgaagcct cggcctgcgg aaggtcgcga catgacgttc cttgcgatcg agaatgtcgg
     2461 caagcgcttc ggccacacca ccgccctcga tggcgtctcg ctaggcatcg agcggggcag
     2521 cttcgttgcc ctcctcggcc cctcgggctg cggcaagtcc acgctgctcc gcatcatcgc
     2581 cggcctcgag acgcccactt tcggccgcat caccgtcgac ggcgaggaca tcacccggct
     2641 gccgcccgaa cgccgccgac tcgccatgat gtttcagtcc tacgccctcc tgccgcacat
     2701 gacggcgctc gagaacgtcc gcttcccgct caggatgcag ggcagggagg gtgcggccga
     2761 gcagcgccgc cgtgcgatgg aaaccctcgc cctcgtcggc ctcgaccacc tggcggagcg
     2821 gctgccgcgc gagatgtccg gcggccagca gcagcgcgtg gccctcgccc gtgccatcat
     2881 cgccgagccg cgcgtgctcc tcctcgacga gccgctctcg aacctcgacg cgcggctgcg
     2941 cgaggacatg cagatcgagc tccaggccct ccaccgccgc ctcggcctga ccaccctctt
     3001 cgtcacccac gaccaggagg aagcgctcag cctcgccgac cgcgtcgtcc tcctgaacca
     3061 gggccgcatc gaccgcgacg ccaccgccga cgccctctac gacgaccccg gcaccgcctt
     3121 cgccgccgac tttttgggtg ctgccaacct cgtccccgtc accgtcgaag ccaccgaaga
     3181 cggccacacc gccatcctcg aggacggcca gcgcctgcag ctcgccagcg cacccggccc
     3241 cggcccccgc cagctcgtcc tccgccagga ggacatcacc ctcgccgacc cgtccgagcc
     3301 cggcctcgaa gccaccatcg aggcccgcat cttcctcggc acccgagtcc gctatcttgt
     3361 ccgcctcggc gacctccgcc tgcgcgtcct cgccccgcgc cacctgcaat acccaccggg
     3421 cacgcccgtg cgcctccaga tcgacccggc aaactgccgc tggagcaggg agggcccttg
     3481 aatcggccgg tcatgtgagt gaggtgaggt ttgtgcagaa tgagcttcgg ctggtgccgc
     3541 aggagggact cgaacccccg accccatcat tacgaatgtc gcggcgccca ggaatccaag
     3601 ctcttgaaat gacaggtttt cgctatctaa cccgttgaat ctagacaagg gcatcttgac
     3661 taatgcggac acggaatgtc aaaagaatga cacgaaaagg cgcccgatag acacttttcg
     3721 tgcggac
//
LOCUS       DAOTFI010000350         3675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_29813_length_3675_cov_6.902762, whole genome shotgun sequence.
ACCESSION   DAOTFI010000350 DAOTFI010000000
VERSION     DAOTFI010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29813_length_3675_cov_6.902762"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="P5558_22805"
     CDS             complement(<1..257)
                     /locus_tag="P5558_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27213.1"
                     /translation="MNELAVIVEAFHFIRPLWLLLLPLIAALWWVVRRARSRRDVPMD
                     GLAPHLRAALTVGGSAYRRLRPIDGVALTLALLALGAAGPT"
     gene            complement(254..1243)
                     /locus_tag="P5558_22810"
     CDS             complement(254..1243)
                     /locus_tag="P5558_22810"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY27214.1"
                     /translation="MISLGFPWALLLLPLPLVFWWLVPAHRERVPALRFPFFRRIVES
                     SGAEPGAGSVVLTRGRLQMAAAITAWCLIVLALAEPERVGEPVEITRAARDVVLAIDI
                     SGSMDAKDFESPDGEGKQRLAGVRDVVGAFVERREGDRVALIVFGSKAYVQSPLTEDL
                     GTITDLLARTEVGMAGPHTALGDAIGLAIRTFEASDIEQRLLILLSDGTDTASRMSPV
                     NAAEIARDNDVEIFTVGVGNPDATGEDRVDLQTLQAIAERTDGAFFFAGDEAALEEVY
                     GRIDELAPREVETVSWRPRQSLAWIPLALAALIGLGTVAFLQLRAGPGRSQPA"
     gene            complement(1240..1719)
                     /locus_tag="P5558_22815"
     CDS             complement(1240..1719)
                     /locus_tag="P5558_22815"
                     /inference="COORDINATES: protein motif:HMM:NF025674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HRY27215.1"
                     /translation="MNGDGATPEITSLVDLIDRLAEPGDPTPVSMVPATAGWTVLAVL
                     LALVALWLVWRGVRRWRANAYRRAALAELAAAGDDPGAVAAILRRTALAAWPRTRVAS
                     LHGGEWLRFLDSTGGDGFTDGPGAVLARTPYRTAGPTPGLGALAARWVRRHRVEEPA"
     gene            complement(1716..2696)
                     /locus_tag="P5558_22820"
     CDS             complement(1716..2696)
                     /locus_tag="P5558_22820"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY27216.1"
                     /translation="MRDQAARIRTGTLPAAPPPGDQDPRVRVTLGHLRSLEGRARGLT
                     FLPRQPARSVLNGRHASRLRGRGLNFEELRDYLPGDDVRAIDWKVTARTGRPHVRVMT
                     EERDRPALIVVDQRMSMFFGSRLNMKSVTAAEAAALAAFRILDQGDRVGGIVFGDERI
                     AEIRPQRTRAALDRFLAALADANGLLRAEAPAVTPISLTRVLRSVARIAPRNHLIIVL
                     SDFDVADAETERLVAGLARRNDLVLGLVSDPFAQDLPKGLKLIVSDGRLQAEIDAGDS
                     TTHRHLSEMASGRLAAILDWQRRFGVPVLPLSTAEETLPQVRRLMGLGPR"
     gene            complement(2716..>3675)
                     /locus_tag="P5558_22825"
     CDS             complement(2716..>3675)
                     /locus_tag="P5558_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY27217.1"
                     /translation="RERMGEAIIGQREVIERLLIGLLANGNLLVEGLPGLAKTRAIKS
                     LARNLECEFSRIQFTPDLLPADVTGTEVYYQGEKGGEFRFEPGPIFANIVLADEINRA
                     PAKVQAALLEAMEERQVTVAGKTHAMTPLFMVMATQNPIEQEGTYPLPEAQMDRFLMH
                     VSITYPPVEDEVEVIRLVRAEETAASAEAAEGTAKEPRQPIPQEAVFAARREIGAIHV
                     ADEMQRYMADLVNATRIPAEFGDDLPRWIEIGASPRASLALDRCGRAHAWLAGRDYVD
                     PEDVRAVAPDVLRHRLKLSYEAQGEGVTPDRVVAEIIRQVALP"
BASE COUNT          511 a         1294 c         1384 g          486 t
ORIGIN      
        1 caggtgggcc cggcggcgcc cagcgccagc agggcgagcg tcagtgcgac gccatcgatc
       61 ggcctgaggc ggcgatatgc cgagccgccg accgtgagcg cggcgcgcag gtgtggcgcc
      121 agcccgtcca tcggcacgtc ccggcgcgac cgcgcgcgac gcacgaccca ccagagtgct
      181 gcgatgagcg gcagcagcag gagccagagc ggccggatga agtggaaggc ttcgacgatg
      241 accgcgagct cgttcatgcc ggctggctcc ggcccgggcc ggcgcgcagc tgcaggaagg
      301 cgacggtgcc gagcccgatc agcgccgcca gcgccagggg gatccaggcg agcgactggc
      361 gcggtcgcca ggagacggtc tcgacctcgc gcggcgccag ctcgtcgatc cgcccgtaga
      421 cctcctcgag cgccgcctcg tcgccggcga agaagaacgc gccgtcggtt cgctcggcga
      481 tcgcctgcag ggtctggaga tcgacgcgat cctcgccggt cgcgtcggga ttgccgaccc
      541 cgaccgtgaa gatctcgaca tcgttgtcgc gcgcgatctc ggcggcgttc acggggctca
      601 tgcggctggc cgtgtcggtc ccgtcggaga gcaggatcag gaggcgctgc tcgatgtcgc
      661 tggcctcgaa cgtgcggatg gcgaggccga tggcatcgcc gagcgccgtg tgcggccccg
      721 ccatgccgac ctcggtgcgc gccaggagat cggtgatcgt tccgagatcc tcggtgagcg
      781 gcgactgcac gtaggccttg gagccgaaga cgatgagcgc cacgcggtcg ccctcgcgcc
      841 gttccacgaa ggcgccgacc acgtcgcgca cgccggccag ccgctgcttg ccctcgccgt
      901 ccgggctctc gaaatccttg gcgtccatgg agcccgagat gtcgatcgcc aggacgacgt
      961 cgcgcgccgc cctggtgatc tcgaccggct cgccgacccg ctccggctcc gccagggcca
     1021 gcacgatcag gcaccaggcg gtgatcgccg ccgccatctg caggcggccc cgcgtcagca
     1081 cgacggaccc ggcgccgggc tcggcaccgc tgctttcgac gatccggcgg aagaagggga
     1141 agcgcagcgc cggcacgcgc tcgcggtgcg ccgggaccag ccaccagaag acgagcggca
     1201 ggggcaaaag gagcagcgcc cagggaaagc cgagggagat cacgccggct cctccacgcg
     1261 gtgccggcgc acccagcggg ccgccagggc gccgaggccg ggcgtcgggc cggccgtccg
     1321 gtagggagtc cgggccagca cggcacccgg cccgtcggtg aacccgtcgc cgcccgtgct
     1381 gtcgagaaag cgcagccatt cgccgccatg caggctcgcc acgcgcgtgc gcggccaggc
     1441 cgcgagggcg gtgcggcgga ggatggcggc aacggcgccc gggtcgtcgc cggcagcggc
     1501 gagctcggcg agcgccgccc ggcgataggc attggcgcgc caccggcgca caccccgcca
     1561 gacgagccag agggcgacca gcgccagcag cacggccagc accgtccagc ccgccgtcgc
     1621 cgggaccatc gagaccggcg tcgggtcgcc gggctcggcc agccggtcga tcaggtcgac
     1681 gaggctcgtg atttcgggtg tcgcgccgtc gccgttcatc gcggccccag gcccatgagg
     1741 cggcgcacct gcggcagcgt ctcctcggcg gtcgagagcg gcaggaccgg cacgccgaac
     1801 cggcgctgcc agtcgaggat cgcggcgagc cgccccgacg ccatctcgct caggtggcga
     1861 tgggtggtgg aatcgccggc atcgatctcg gcctggagcc ggccgtccga gacgatgagc
     1921 ttcaggccct tcggcaggtc ctgggcgaag gggtccgaca cgaggccgag cacgaggtcg
     1981 ttgcgtcgtg ccaggccggc gaccagccgc tcggtctccg cgtcggccac gtcgaagtcg
     2041 ctgagcacga tgatcaggtg gttgcggggg gcgatccggg cgacgctgcg cagcacgcgg
     2101 gtgagcgaga tcggcgtcac cgccggtgcc tcggcgcgca gcaggccgtt ggcgtcggcg
     2161 agggccgcga ggaagcggtc gagcgcggcc cgcgtgcgct gcggccggat ctcggcgatc
     2221 cgttcgtcgc cgaagacgat gccgccgacc cggtcgccct ggtcgaggat gcgaaaggcg
     2281 gcgagcgccg ccgcctcggc ggcggtcacc gacttcatgt tgagcctaga gccgaagaac
     2341 atcgacatgc gctggtcgac cacgatcagc gccggccggt cgcgctcctc ggtcatcacg
     2401 cggacatgcg gccggccggt ccgggccgtc accttccagt cgatggcgcg gacgtcgtcg
     2461 cctggcaggt agtcgcgcag ctcctcgaaa ttgagcccgc gaccgcgcag ccgcgatgcg
     2521 tgtcggccat tgagcacgga gcgggccggc tggcggggca ggaaggtgag gccccgggca
     2581 cggccctcga gcgagcgcag atggccgagc gtcacgcgga cgcgcggatc ctggtcgccc
     2641 ggcgggggtg ccgcgggcaa ggtgccggtc ctgatccgcg ccgcctggtc gcgcatcgcc
     2701 gtctccctca gagcctcagg gcagcgccac ctgcctgatg atctcggcca cgacccgatc
     2761 gggcgtgacc ccttcgccct gggcctcgta gctcagcttg aggcggtggc gaagcacgtc
     2821 gggcgccacg gcgcgcacgt cctcggggtc gacatagtcg cggccggcga gccaggcgtg
     2881 ggcgcgaccg cagcggtcga gcgccaggct cgcgcgcggg ctggcgccga tctcgatcca
     2941 gcgtggcagg tcgtcgccga actcggccgg aatgcgcgtg gcgttgacca gatcggccat
     3001 gtagcgctgc atctcgtcgg cgacgtggat ggcgccgatc tcgcgccgcg ccgcgaacac
     3061 ggcttcctgc gggatcggct ggcggggctc ttttgccgtg ccttcggccg cctcggcgct
     3121 ggccgcggtc tcctcggcgc gcacgaggcg gatgacctcg acctcgtcct cgaccggcgg
     3181 gtaggtgatg ctgacatgca tcaggaagcg gtccatctgc gcctcgggca aggggtaggt
     3241 gccctcctgc tcgatcgggt tctgggtcgc catgaccatg aagagcgggg tcatcgcatg
     3301 ggtcttgccg gcgaccgtca cctggcgctc ctccatggcc tcgaggaggg cggcctgcac
     3361 cttggccggt gcccggttga tctcgtcggc gagcacgatg ttggcgaaga tcgggccggg
     3421 ctcgaagcgg aactcgccgc ccttctcgcc ctggtagtag acctcggtgc cggtgacgtc
     3481 ggccggcagg aggtcgggcg tgaactggat gcggctgaac tcgcactcga ggttgcgggc
     3541 gagcgacttg atcgcccgcg tcttggcgag gccgggcagg ccctccacca ggaggttgcc
     3601 attggcgagc aggccgatga gcaggcgctc gatcacctcg cgctggccga tgatcgcctc
     3661 gcccatgcgc tcgcg
//
LOCUS       DAOTFI010000351         3670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_29864_length_3670_cov_6.794744, whole genome shotgun sequence.
ACCESSION   DAOTFI010000351 DAOTFI010000000
VERSION     DAOTFI010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29864_length_3670_cov_6.794744"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="P5558_22830"
     CDS             <1..198
                     /locus_tag="P5558_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRY27218.1"
                     /translation="QKGVAFDEINVLMAAGRRQEMVERAGGRSTVPQIFIDGRGIGGC
                     DDLMALDRAGGLDPLLEGAAG"
     gene            195..710
                     /locus_tag="P5558_22835"
     CDS             195..710
                     /locus_tag="P5558_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRY27219.1"
                     /translation="MIRYALRCGAGHEFEGWFRSSAAFGEEAAALACPICGDGGIEKA
                     IMAPAVMQRATPSAEPELQPAPAAAPPAGGAVAVAGEFAAPAMPAAALTPRQVELARA
                     LVAMRRLKAHVEAHFENVGKRFAEEARKIHHEEAEARDIFGQATLEEARELLEEGIEV
                     LPLPDLPKLDG"
     gene            complement(798..1646)
                     /locus_tag="P5558_22840"
     CDS             complement(798..1646)
                     /locus_tag="P5558_22840"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY27220.1"
                     /translation="MAPAGPGLWGGGDAYEHYMGRWSRLVARVFVDWVGAPAGARWID
                     IGCGTGILTTAVLDRCAPSRVLGVDSSPAFLEAARSQVLDPRAAFEPGDAQAIPAGDA
                     AFDVAAAGLLLNFVPDPGRALREMVRVVENGGRVALYVWDYPGHMQIMRHFFDVATAL
                     DPGARDFDDGVKAPFCRPAPLAALLAGAELAMVETRAIDIPTAFAGFDDYWTPFLGGT
                     GSAPRYCMSLSEAARDRLREALRQRLPTGPDGEILLAARAWAARGRKVDQSVGTAAVD
                     EEGPLD"
     gene            complement(1659..2450)
                     /gene="ubiG"
                     /locus_tag="P5558_22845"
     CDS             complement(1659..2450)
                     /gene="ubiG"
                     /locus_tag="P5558_22845"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HRY27221.1"
                     /translation="MGKQLTGIARDAPPQPQGASALDRDEVAKFEALAEEFWDPSGPM
                     RPLHKLNPLRIAYIRDHATRLLAPAGAGAGGLRPLAGLRLADIGCGAGLLSEPMARLG
                     AEVTGVDPSPRSIEIASAHAAAAGLAIDYRATNVETLAEAGELFDVVLAMEVIEHTTD
                     PDLFICELADITRPGGLVVLSTLNRTARSWLLGIVAAEYILGWLPRGTHQWQRFVPPA
                     TLARLLRRRGLRVIDTQGVVYRPHRDDFVTSRDLSVNYMISAVKD"
     gene            2555..>3670
                     /locus_tag="P5558_22850"
     CDS             2555..>3670
                     /locus_tag="P5558_22850"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRY27222.1"
                     /translation="MTLVVQKYGGTSVADLERIRHVAGRVARDVRAGERVCVVLSAMA
                     GETNKLVGWVDGLDPANYDPAEYDVVVSSGEQVTVGLLAIALQSIGVPARSFTGWQAG
                     VATDGAHGKARITGIDKAPILAALEAGKVAVVAGFQGLAPGDRLATLGRGGSDTSAVA
                     LAAAIGADRCDIFTDVDGVYTSDPRIVTKARKLDRITYEEMLELASQGAKVLQTRSVA
                     LAMAHKVKVQVLSSFSDEPGTLVVDEDDIVEQQVVSGIAYSRDEARLTVLGVRDRPGI
                     AAAVFGPLAAAGINVDMIVQSVSRDGVNANMTFTVTRSDLKRAQAVLAEHQATIGFGE
                     ILVDDDVSKVSVVGVGMRSHAGIAHTMFQTLADKGINI"
BASE COUNT          545 a         1237 c         1399 g          489 t
ORIGIN      
        1 cagaagggcg tcgccttcga cgagatcaac gtcctgatgg cggccgggcg gcggcaggag
       61 atggtggagc gggccggggg acggagcacg gtcccgcaga tcttcatcga cggccgcggg
      121 atcggcggct gcgacgacct catggcgctc gaccgtgcgg gcgggctcga cccgctgctc
      181 gaaggggcgg ccgggtgatc cgctacgcgc ttcgctgcgg cgccgggcac gagttcgagg
      241 gctggttcag gagcagcgcc gcgttcggcg aggaggcggc cgcgctcgcc tgcccgatct
      301 gcggtgacgg cggaatcgag aaggcgatca tggcgccggc ggtgatgcag cgcgccacgc
      361 cgtcggccga gcccgagctc cagccggccc ctgcggcagc accgccggcg ggcggggcgg
      421 tcgcggtcgc gggcgagttc gcggcaccgg ccatgccggc ggcggcgctg acgccgcgcc
      481 aggtcgagct ggccagggcg ctcgtggcga tgcggcggct caaggcgcat gtcgaggcgc
      541 atttcgagaa tgtcggcaag cgcttcgccg aggaggcgcg caagatccac cacgaggaag
      601 ccgaggcgcg cgacatcttc gggcaggcga cgctggagga ggccagggag ctgctcgagg
      661 agggcatcga ggtgctgccc ttgcccgacc tgcccaagct ggacggctga gcgtcggcac
      721 ggcggccgta tacttcgtca ggccgcggac gatcggtgct cacgtaggta aactacgctg
      781 cgcgccgatc gccccagtca gtccaatggg ccttcctcgt ctacggccgc cgtgccgacg
      841 ctctgatcga ccttgcggcc tcttgcggcc cacgcccgcg ccgccagcag gatctcgcca
      901 tcggggccgg tcggcaggcg ctggcgcagc gcttcccgca gccggtcccg tgcagcttcg
      961 ctcagggaca tgcagtagcg gggtgcggag ccggtgccgc cgaggaaggg cgtccagtaa
     1021 tcgtcgaagc cggcgaaggc ggtcgggatg tcgatggccc gcgtctccac catggcgagc
     1081 tcggcgccgg cgagcagggc cgccaggggc gccgggcggc agaagggcgc cttgacgccg
     1141 tcgtcgaagt cgcgtgcccc gggatcgagg gcggtagcga cgtcgaagaa gtgccgcatg
     1201 atctgcatgt gaccggggta gtcccagaca tagagggcga cccggccgcc gttctcgacg
     1261 acccggacca tctcgcggag cgcccggccc gggtccggga cgaagttgag caggagcccg
     1321 gcggccgcga cgtcgaaggc ggcgtcgccg gccgggatcg cctgggcgtc gcccggctcg
     1381 aaggcggcgc ggggatcgag gacctgcgag cgggccgcct cgaggaaggc cgggctgctg
     1441 tcgacgccga ggacgcgaga cggcgcgcag cgatcgagca cggccgtggt cagaatgccg
     1501 gtgccgcagc cgatgtcgat ccagcgcgca cccgcggggg cgccgaccca gtccacgaag
     1561 acccgggcga cgagccggct ccagcgaccc atgtaatgct cgtaggcgtc gccgccaccc
     1621 cagaggccgg gtcctgcagg cgccatcggt ggtgcccctc agtccttgac ggcactgatc
     1681 atgtagttga ccgagagatc ccggctggtg acgaaatcgt cgcggtgcgg gcggtagacg
     1741 acgccctgcg tgtcgatgac ccggaggccg cggcggcgga gcaggcgggc gagggtggcg
     1801 ggcggcacga aacgctgcca ctggtgcgtg ccgcgcggca gccagccgag gatgtattcg
     1861 gcggcgacga tgccgaggag ccacgagcgg gcggtgcggt tgagggtcga gagcacgacg
     1921 agcccgcccg ggcgggtgat gtcggcgagc tcgcagatga agaggtcggg gtcggtggtg
     1981 tgctcgatca cctccatcgc caggaccacg tcgaagagct cgccggcttc ggccagcgtc
     2041 tcgacattcg tcgcccggta gtcgatggcg aggccggcgg cggcggcgtg ggcgctcgcg
     2101 atctcgatcg agcgcggcga cgggtcgacc ccggtcacct cggcgccgag ccgcgccatg
     2161 ggctcgctca gcagcccggc cccgcagccg atatcggcga gcctgaggcc ggcgagcggc
     2221 cgcagcccgc ccgcacccgc ccccgccgga gccagcagcc gcgtcgcgtg gtcacggata
     2281 taggcgatgc gcagggggtt gagcttgtgc aggggccgca tcggccccga cgggtcccag
     2341 aattcctcgg ccagcgcctc gaacttcgcc acctcgtccc ggtcgagagc ggaagcaccc
     2401 tggggctgcg gaggcgcatc ccgagcgatg ccggtcagtt gcttgcccat tggactcctg
     2461 ctggctagaa ggtggcgcat ccggcgagct gccccggcca cggcccctga cgttttcctg
     2521 gccgcgcggg cgccatccga agtgagtaac atccttgacg ctggtcgtgc agaaatatgg
     2581 cggcacctcg gtcgccgatc tagagcgcat tcgccatgtg gcgggccgcg tggcccgcga
     2641 tgtccgggcg ggcgagcgcg tctgcgtggt gctctcggcc atggccggcg agaccaacaa
     2701 gctcgtcggc tgggtcgacg ggctcgatcc ggccaactac gacccggccg agtacgacgt
     2761 cgtcgtctcc tctggcgagc aggtgacggt cgggctcctg gcgatagcgc tgcagtcgat
     2821 cggcgtgccg gcccgctcgt tcaccggctg gcaggccggg gtggcgaccg acggggcgca
     2881 cggcaaggcg cggatcaccg gcatcgacaa ggcgcccatc ctggcggcgc tcgaggccgg
     2941 caaggtggcc gtggtcgccg ggttccaggg cctggcgccg ggcgaccggc tggccacgct
     3001 cggccgcggc gggtccgaca cctcggccgt cgccctggcg gcggcgatcg gtgcggatcg
     3061 ctgcgacatc ttcaccgacg tggacggcgt ctacacgagc gatccccgga tcgtgacgaa
     3121 ggcgaggaag ctcgaccgca tcacctacga ggagatgctc gagctggctt cgcagggggc
     3181 gaaggtgctg cagacccgct cggtcgccct cgccatggcg cacaaggtga aggtacaggt
     3241 actctccagc ttcagcgacg agcccggcac gctcgtcgtc gacgaggatg acatcgtgga
     3301 acagcaagtc gtcagcggca tcgcctacag ccgggacgag gcccggctca ccgtgctcgg
     3361 cgtgcgcgac cggccgggga tcgcggcggc cgtgttcggg ccgctcgccg cggccggcat
     3421 caatgtcgac atgatcgtcc agtcggtgag ccgcgacggc gtcaacgcca acatgacctt
     3481 cacggtgacg cgcagcgacc tcaagcgggc gcaggccgtg ctggcggagc accaggcgac
     3541 catcggcttc ggcgagatcc tcgtcgacga cgacgtcagc aaggtctcgg tggtcggcgt
     3601 cggcatgcgc agccatgccg gcattgccca caccatgttc cagacgctgg ccgacaaggg
     3661 catcaacatc
//
LOCUS       DAOTFI010000352         3664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_29931_length_3664_cov_5.578554, whole genome shotgun sequence.
ACCESSION   DAOTFI010000352 DAOTFI010000000
VERSION     DAOTFI010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29931_length_3664_cov_5.578554"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="P5558_22855"
     CDS             <1..1137
                     /locus_tag="P5558_22855"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRY27223.1"
                     /translation="GTVSVIVDQQGFAPIVERVALHHPQYLALQAALQRYRAILAAGG
                     WSPVPQGPTLRPGTIDPRVPDLRARLLVTGELAGGSAMAAGDLFDDPLEAAVRRFQVR
                     HGLDPDGLVGPGTLRALNRTVGERIDQLRLSLERARWVLRGIGDDFVLVNIAGARTYV
                     YKGGDLVWRTRSIVGQQYRRTPIFRDEIRYMELNPTWTVPRTIFVEDKLPLIRADPGY
                     LARGGYRVLDRDRQVLDPSVVDWWALDPPPVTLVQAPGPGNALGRVKFMFPNRFAVYL
                     HDTNDRSLFDRAARNLSSGCVRIEQPFVLADLLMEGDPTWSHARLEAILASGTTTRVD
                     LPKPMPVLLTYYTAWVEDGEVLFREDIYDRDERVLEALDGPFRG"
     gene            1286..1663
                     /locus_tag="P5558_22860"
     CDS             1286..1663
                     /locus_tag="P5558_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRY27224.1"
                     /translation="MDEKLFEKGLRARKATLGEAYVERNLAAADDFTRPFQEAMTAWA
                     WGFGWGDETIDAKTRSMMNLAMIGALGKMREWEIHCRGALNNGVSKAEIRAIIHVVGI
                     YCGVPQALECFRVARKVLEEEGV"
     gene            complement(2161..3000)
                     /locus_tag="P5558_22865"
     CDS             complement(2161..3000)
                     /locus_tag="P5558_22865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRY27225.1"
                     /translation="MHVFRSDWEPAPRAAPPGATLLAIGDVHGCPAQLDAMLAVLAEV
                     VDDARRRGRRAELVMLGDYVDRGPDSPGVLRRLPDIGRRLEPEVPVHLLLGNHDELLL
                     DRLGEPPDPAALGHWLRRGGSRLPGMTPAPDLVAPPTDEEQPDLATLAALLSGLASHR
                     VIGDYVFAHAGIAPDRPLAAQTLDDLLWIREPFLDATDWPHGFAVVHGHTPQGPEVLP
                     HRVGVDSGCFFTGVLTAVQIEDRRLRFHAVSADPEPLEFRELIEASGQRRRFAGPTPL
                     PGS"
     gene            3124..3606
                     /locus_tag="P5558_22870"
     CDS             3124..3606
                     /locus_tag="P5558_22870"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRY27226.1"
                     /translation="MANDYHLTSQWRVRGSPDEVAAILREPMDLVRWWPEVYLDVRVE
                     EADGGPVVHVLTRGFLPYMLSWQFREVERDWPRGASIEAHGDLVGRGTWTLVREGPET
                     VATYDWRVRAEKPWLRRLAPLLQPLFAWNHRWAMAKGEAGLQRELERRRAMVHGASLY
                     "
BASE COUNT          526 a         1155 c         1378 g          605 t
ORIGIN      
        1 gggacggtca gcgtgatcgt cgatcagcag ggcttcgcgc cgatcgtcga gcgggtcgcc
       61 ctccaccacc cgcaatacct ggccctgcag gcggccctgc agcgctatcg ggcgatcctc
      121 gcggcgggcg gctggtcgcc tgtgccgcag ggcccgacct tgcggccggg gacgatcgac
      181 ccgcgcgtgc cggatctccg ggcccggctg ctggtcacgg gcgagctggc gggcgggtcg
      241 gccatggcgg cgggtgatct cttcgatgat ccactcgagg cggcggtgcg gcggttccag
      301 gtgcgccacg gactcgatcc ggacgggttg gtcggcccgg ggacgctccg ggccctgaac
      361 cggacggtgg gcgagcggat cgaccagctc cggctcagcc tcgagcgggc gcgctgggtg
      421 ctgcgcggca tcggcgacga cttcgtgctc gtcaacatcg ccggagcgcg gacctatgtc
      481 tacaagggcg gcgatctcgt ctggcggacc cggtcgatcg tcggccagca ataccgccgc
      541 acgccgatct tccgggacga gatccgctac atggagctca accccacctg gaccgtgccc
      601 cgcacgatct tcgtcgagga caagctgccg ttgatccggg ccgaccccgg ctatctggcg
      661 cggggcggct atcgggtcct cgaccgcgac cggcaggtcc tcgacccctc cgtggtcgac
      721 tggtgggcgc tcgacccgcc gcccgtgacc ctggtccagg cgccggggcc gggcaacgcg
      781 ctgggccggg tcaagttcat gttccccaac cgcttcgccg tctacctgca cgacaccaac
      841 gaccgcagcc tgttcgaccg ggcggccagg aacctgagct ccggctgcgt gcggatcgag
      901 cagcccttcg tcctcgccga tctcttgatg gaaggcgatc cgacctggag ccatgcccgg
      961 ctggaggcga tcctggcgag cggcacgacc acgcgggtcg acctgccgaa gccgatgccg
     1021 gtgctcctga cctactacac cgcctgggtc gaggacggcg aggtgctgtt ccgcgaggac
     1081 atctacgacc gcgacgagcg ggtgctggaa gccctcgacg gccccttccg cggctgaggg
     1141 gcccgaggat ggccgcatac ttcgtcaggc cgcggtcgat cggtgctcac gcaggtagac
     1201 tgcgctgcgc gccgctcgcc ttgccttcct cgtctgcggt catcctgggg cccctctggc
     1261 gacctgctca tttggagaga gaaggatgga cgagaagctg ttcgagaagg ggttgcgggc
     1321 gcggaaggcg acgctgggcg aggcttatgt ggagcggaac ctcgccgcgg cggacgactt
     1381 cacgcggccg ttccaggagg cgatgacggc ctgggcctgg ggcttcggct ggggcgacga
     1441 gacgatcgac gcgaagaccc gctcgatgat gaacctcgcc atgatcgggg cactcggcaa
     1501 gatgcgcgaa tgggagatcc attgccgggg ggcgctcaac aatggcgtca gcaaggcgga
     1561 gatccgggcg atcatccatg tcgtcggcat ctattgcggc gtgccgcagg cgctcgagtg
     1621 ctttcgcgtg gcgcggaagg tgctggagga ggaaggggtg tgacggcgcc ggcgggccat
     1681 cactgatata ggaataatag cctattttgc gtccaggcgc aagcctgaag ccggtggctt
     1741 tcgggttcga tcggcatttt ccggttcgtg ggttcgctgg cgccggccgg atgggtgcct
     1801 gatcgtcgag aggcgtgttt tttcaacggg ttgaggggcg ccgggttcgt tggctcgggc
     1861 tcgggttcga tcgtcgtttc ggtgggttcg atcgtcgaaa tcgcgggttc gttcgtcgat
     1921 tccgcgggtt cgttggctgt gcgatgtcgc ggaatccgcg ccatcacgaa tcgttatgtc
     1981 gcgtttatcg cgacattttc gggcgggctg tcgcggaatc cgcgacaacc cgagccgatt
     2041 ggttgcattg gatgcgccaa cccgggcccg ttgcaggtcc aaagtgcccc gagccaggtg
     2101 gtcggggagc tcgaccgggg ggcgggcata agcattcgtg atgaggtgaa ccggtgccct
     2161 tcaggagccc ggcaggggcg tcgggccggc aaaccgccgc cgttggccgc tggcctcgat
     2221 cagctcccgg aattccagcg gctccggatc ggcggagacg gcgtggaagc gcaggcgtcg
     2281 atcctcgatc tggaccgccg tcagcacgcc ggtgaagaag cagccgctgt cgacgccgac
     2341 cctgtgcggc agcacctcgg gcccctgcgg ggtgtggccg tggaccacgg cgaagccatg
     2401 gggccagtcg gtggcgtcga ggaagggctc gcggatccag aggaggtcgt cgagggtctg
     2461 ggctgcgagt ggccggtccg gggcgatgcc ggcatgggcg aagacgtagt cgccgatgac
     2521 ccggtggctg gcgagcccgg ataggagggc ggccagggtc gcgaggtccg gttgctcctc
     2581 gtcggtcggg ggagcgacca ggtccggggc gggggtcatg ccgggcagcc ggctgccgcc
     2641 ccgccggagc cagtggccca gtgcggcagg atctggcggc tccccgagac gatcgaggag
     2701 gagctcgtcg tgattaccga gcagcaggtg caccggcacc tccggctcga gccgccggcc
     2761 tatgtcgggc agacgtcgta gcacgcccgg gctgtccggg ccccggtcga catagtcgcc
     2821 gagcatgacc agctccgcgc ggcgaccccg gcggcgggcg tcgtcgacga cttccgccag
     2881 caccgccagc atggcgtcga gctgggccgg gcagccgtgc acgtcgccga tggcgagcag
     2941 cgtggcaccc ggtggcgccg ctcggggtgc tggctcccag tcggagcgaa agacgtgcat
     3001 gcaagttctc caggaaaccg gcactttttt tggctttcag gatttgccgg aagtccacag
     3061 gctggaccag agggagttgc gggcggcttc ctgcgagggg cgggaatcgg ctaggatggc
     3121 cgcatggcaa acgactacca tctgaccagc caatggcggg tgcggggctc gcccgacgag
     3181 gtggccgcca tcctgcgcga gcccatggat ctcgtgcgct ggtggccgga ggtctatctg
     3241 gacgtgcggg tcgaggaggc ggatggcggg ccggtcgtcc acgtcctgac gcgcggcttc
     3301 ctgccctaca tgctaagctg gcagtttcgc gaggtggaac gtgactggcc gcgcggggcg
     3361 tcgatcgagg ctcatggcga tctcgtcggc cgtggcacct ggacgctggt gcgggagggg
     3421 cccgagacgg ttgccaccta tgactggcgg gtgcgggccg agaagccctg gcttcgccgc
     3481 ctcgcaccgc tcctccagcc gctcttcgcc tggaaccacc gctgggccat ggccaagggc
     3541 gaggccggcc tgcagcgcga gctggaacgc cggcgtgcga tggtgcatgg ggcttcgttg
     3601 tattgacggg ggaggcggcg aatgctgacg atgggggcgg tcatctccag ctctaaagag
     3661 tcct
//
LOCUS       DAOTFI010000353         3641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_30155_length_3641_cov_5.945343, whole genome shotgun sequence.
ACCESSION   DAOTFI010000353 DAOTFI010000000
VERSION     DAOTFI010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30155_length_3641_cov_5.945343"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1097)
                     /locus_tag="P5558_22875"
     CDS             complement(72..1097)
                     /locus_tag="P5558_22875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY27227.1"
                     /translation="MSSLMLDEVRETPSLVAAQLAADGEAYASLAASLAAAPPVFAAT
                     IARGSSDHAASYAATVLAHAMGLATASLAPSLVSRYGVELALDKAFVLGLSQSGASPD
                     LVASMQAAAASGAVTAAIVNQPDSPLAEAAGHVLPQRAGAERSVAATKSFILTLVAIA
                     RLAAVWRGDAALRAGLERLPERLEAALAVNWGGTVDTLAEASSLYVLGRGPALSIANE
                     AALKLKETSYLHAEAFSSAEVRHGPRAVIDDGFPVLALALADAGGADTAAFAAEMAAS
                     GGTVITASPDPDVAGQAVRLPEPLHPLLDPIVAITAFYPMAATLAAARGHDPDKPRGL
                     KKVTSTV"
     gene            complement(1112..2020)
                     /locus_tag="P5558_22880"
     CDS             complement(1112..2020)
                     /locus_tag="P5558_22880"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRY27228.1"
                     /translation="MGRTRYILAVDGGGTKTRAALAPWEGEVVAEATGGPCNLYQDPA
                     GGLAEIRALWQRLCATAGLDPAGAAADTAISAGLAGTSAPGSGDRFRAAFADFATRHL
                     SSDGYVALIGAVAGRPGALLSIGTGVVGFRLDASGAHRQLSGWGFPVGDRGGGAWLGL
                     RAIGDWLERLDGYGGDAASTLWPLLQDRLGSSPADILAWLKGARPAEFASLAPFVVER
                     AAAGDPHARSLIDEAAGHHVRLARALAATATEPLVLAGGLAEVFRPAIAAALGPALDQ
                     SGRIPSPLAGALLIARGRNAAEFPEA"
     gene            2205..3107
                     /locus_tag="P5558_22885"
     CDS             2205..3107
                     /locus_tag="P5558_22885"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRY27229.1"
                     /translation="MDTERRLERYRAADRWPTEEALAAMLDNQMGAFAAVRQALPALA
                     QAVRAAAERLRNDAGRLVYVGAGASGRLAVQDGVELYPTFDWPHDRLIYIVAGGEAAL
                     VQSREGAEDDAEAGAGAIREHAIGPADVVLGIAASGTTAFTRAAIRTARERGALTIGM
                     ANNPAAPLLNEAEHALLLATGPEFLAGSTRMTAGTAQKLALNLFSTQTMTELGRVYDG
                     LMVNVVASNAKLVERGRRMVEAITGCDEATASEAHSAAGHDVKLAVLLVDGMTLEAAR
                     RCLARAKGDLRRARDLEPEPIHET"
     gene            3173..>3641
                     /locus_tag="P5558_22890"
     CDS             3173..>3641
                     /locus_tag="P5558_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY27230.1"
                     /translation="MRTTSTAAIALSIGLIATSASAAELVIESWRNDDITIWQDTILP
                     AFHAQHPDIQVTFQPTAPAEYNAALNAKLDGGTAGDIITCRPFDASLEMFKRGHLVDL
                     TGMEELGNFPETALAAWSTDDGSQTFCVPMASVIHGFMYNKDAFAELGLEVPTT"
BASE COUNT          556 a         1260 c         1357 g          468 t
ORIGIN      
        1 tcacccatgt cctcgaacgc atgtaaacct tgtctccggt ctatacagcc tccccgactc
       61 ttcatccttt cctagaccgt cgacgtcacc ttcttcagcc ccctcggctt gtccgggtcg
      121 tggccgcggg cggcggccag ggtggcggcc atggggtaga aggcggtgat ggcgacgatg
      181 gggtcgagga gcgggtggag gggctcgggc aggcgcacgg cctggccggc gacgtcgggg
      241 tcggggctgg cggtgatcac ggtgccaccg gaggctgcca tctcggcggc gaaggcggcg
      301 gtgtcggcgc cgccggcgtc ggcgagggcc agggccagga cggggaagcc gtcgtcgatg
      361 acggcccggg ggccgtgccg cacctcggcg gagctgaagg cctcggcgtg gagataggag
      421 gtttccttga gcttgagggc ggcctcgttg gcgatcgaga gggccgggcc gcggccgagc
      481 acgtagaggc tcgacgcttc ggccagggtg tcgacggtgc cgccccagtt gaccgcgagc
      541 gctgcctcga gcctttcggg caggcgctcg aggccggccc gaagcgcggc gtcgccgcgc
      601 cagaccgcgg cgaggcgggc gatggcgacg agggtgagga tgaagctctt ggtggcggcg
      661 acgctgcgct cggcgccggc gcgttgcggc aggacgtgcc cggcggcctc ggccagcggg
      721 gagtcaggct ggttgacgat cgccgccgtc accgccccgg acgcggccgc ggcctgcatg
      781 ctggccacga ggtcggggct ggcgccggac tgcgagaggc cgagcacgaa ggccttgtcg
      841 agcgccagct cgaccccgta gcggctgacc agggagggtg cgagggaggc ggtggcgagg
      901 cccatggcgt gcgccagcac ggtggcggcg tagctcgccg cgtggtcgga gctgccgcgc
      961 gcgatggtcg cggcgaagac cggcggggcg gcggccaggg aagcggcgag cgatgcatag
     1021 gcctcgccat ccgccgcgag ctgtgccgcc acgagcgaag gcgtttcgcg cacctcgtcg
     1081 agcatgaggg acgacatggg aggtcctttc gtcaggcttc ggggaactcc gcggcatttc
     1141 ggccgcgggc gatgaggagg gcgccggcga gcggcgaagg tatccggccg ctctggtcga
     1201 gcgccgggcc gagcgctgcg gcgatggcgg gccggaagac ctccgcgagg ccgccggcga
     1261 ggacgagcgg ctccgtcgcc gtggcggcga gggcccgggc gagacggacg tggtggcccg
     1321 cggcctcgtc gatcagcgat cgggcatgcg ggtcgccggc ggcggccctt tcgacgacga
     1381 agggtgcgag gctcgcgaat tcggccggcc gcgccccctt gagccaggcg aggatgtcgg
     1441 caggcgagga gcccaggcga tcctgcagca gcggccagag ggtgctcgcc gcatcgccgc
     1501 catagccgtc gagccgctcc agccagtcgc cgatggcccg aaggccgagc caggcgccac
     1561 cgccccggtc gccgaccgga aagccccagc cgctgagctg gcgatgcgcg ccggaggcgt
     1621 cgaggcggaa gccgaccacg ccggtgccga tggagagaag ggcaccgggc cggcccgcga
     1681 cggcgccgat cagcgccacg tagccgtcgc tcgagagatg gcgagtggcg aaatcggcga
     1741 aggccgcgcg gaaccgatcg ccgctgccgg gagcgctggt gccggcgagc ccggcgctga
     1801 tggcggtgtc ggccgcggcg ccggccgggt cgagccccgc cgtggcgcag aggcgctgcc
     1861 agagggcgcg gatctcggcg aggccgccgg ccgggtcctg gtagaggttg cacggcccgc
     1921 cggtcgcctc ggcgacgacc tcaccttccc agggggcgag cgctgcccgg gtcttggtgc
     1981 cgccgccgtc gacggcgagg atatagcgtg tcctgcccat gcagcgtgca taccaaaagg
     2041 gaccagaact ggcaatccgc aatcggcgac aggggaagcc gacacgtatt atctcgataa
     2101 aacaatggat taatagattc ggcagaaatt gataaccatg tcgaagaata cctcttgccg
     2161 agtggtattt ttttggtatg gtccagccga caacggaggc agggatggat acggaacgac
     2221 gcctggagcg ctaccgcgcg gccgatcgct ggccgaccga ggaggcgctc gccgccatgc
     2281 tcgacaacca gatgggcgcc ttcgccgccg tgcggcaggc tctgccggcc ctcgcccagg
     2341 cggtacgggc cgccgccgag cgtctgcgca acgacgccgg ccggctggtc tatgtcggcg
     2401 ccggcgcctc gggccggctc gccgtgcagg acggcgtcga gctctatccg accttcgact
     2461 ggccgcacga ccggctgatc tacatcgtgg ccggcggcga ggccgccctg gtccagtcgc
     2521 gcgagggcgc ggaggacgac gccgaggccg gcgccggcgc cattcgcgag cacgccatcg
     2581 gcccggccga cgtggtcctc ggcatcgccg cctcgggcac caccgccttc acccgcgccg
     2641 ccatcaggac cgcccgggag cgcggtgcgc tgaccatcgg catggccaac aacccggccg
     2701 caccgctgct gaacgaggcc gagcacgcgc tgctcctcgc caccggcccg gagtttctcg
     2761 ccggctccac ccggatgacc gccggcacgg cgcagaagct ggcgctcaac ctcttctcga
     2821 cccagaccat gacggagctc ggccgcgtct atgacgggct catggtcaat gtcgtcgcct
     2881 cgaacgcgaa gctggtcgag cgcggccggc gtatggtcga ggcgatcacc ggctgcgacg
     2941 aggcgaccgc gagcgaggcg cacagcgctg ccggccacga cgtcaagctc gccgtcctcc
     3001 tggtggacgg catgaccctc gaggcggccc gtcgctgcct cgcccgggcg aaaggcgacc
     3061 tgcgccgggc ccgggacctc gagcccgagc cgatccacga gacctgactc gcgatctccg
     3121 acctgcgaac caagccaagc aacccagccc ggaaaccctg gaggcaactg ccatgcgaac
     3181 gacctcgacc gctgccatcg ccctctccat cggcctgatc gcgacgagcg ccagtgccgc
     3241 ggagctggtc atcgagagct ggcgcaacga cgacatcacc atctggcagg acaccatcct
     3301 gccggccttc catgcccagc accccgacat ccaggtgacg ttccagccga cggcaccggc
     3361 cgagtacaat gcagcgctca acgccaagct ggacggcggc accgccggcg acatcatcac
     3421 ctgccggccg ttcgacgcga gcctcgagat gttcaagcgc ggccacctcg tcgacctgac
     3481 gggcatggag gagctcggca acttccccga gacggcgctc gccgcctgga gcacggacga
     3541 cggcagccag accttctgcg tgccgatggc ctcggtgatc cacggcttta tgtacaacaa
     3601 ggacgccttc gccgagctcg gcctcgaggt gccgacgacg g
//
LOCUS       DAOTFI010000354         3599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_30610_length_3599_cov_7.628950, whole genome shotgun sequence.
ACCESSION   DAOTFI010000354 DAOTFI010000000
VERSION     DAOTFI010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30610_length_3599_cov_7.628950"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="P5558_22895"
     CDS             complement(<1..510)
                     /locus_tag="P5558_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY27231.1"
                     /translation="MDPDCSTFFCQAWTARWSLLEGLMVTISSSIMTIVIGTVLGFVV
                     GLILTYGMLPLRLLARLYVDVMRGIPVLVLILFTYYGLAILKIGVKPYWAGVIALSLF
                     CVSHVAENVRGAVQSIPQGQHEAGMAIGMRFFQRLRWIILPQAVRRALPPWVNTALEI
                     VKGTTLTISR"
     gene            complement(529..1197)
                     /locus_tag="P5558_22900"
     CDS             complement(529..1197)
                     /locus_tag="P5558_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY27232.1"
                     /translation="MIAYNFNWAVIWRNWDAFLGALALGLGLAVVSLVIGTIIGLFLA
                     YARLSPSPWISRPAWAYVEFIRNTPLLLLIFFVYFGLPEIGIYNLDKIQSFILTLSGY
                     AAAYMCEVFRAGLASMPRAYGEAAKAIGLRPWQRQRHVVLPVMFRITLPAVSNNLISL
                     FKDTSLAAAIAVPELTFTARQINANTYRVIECWLTASALYLVTAYAIAIALRQVEKRY
                     AVVR"
     gene            complement(1238..2158)
                     /locus_tag="P5558_22905"
     CDS             complement(1238..2158)
                     /locus_tag="P5558_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY27233.1"
                     /translation="MGELRDLAFSMLKRRGLLGMGAVAGGAAGLAGGVAGLVAPRAAQ
                     AQPVDPSASKLQQVLDRGHLIVGTGSTNPPWHFEDETGTLVGMDIDMAHLIAKNLFDD
                     PTKVEFVLQGSDARIPSLITDQVDVICQWMTITPGRAQQCAFTVPYYREGIGLLVMAE
                     GARFTTFEDMLAAGADARVGAMQNVFIEDWIHAGLPEATVDQFDSPDSTLQALNARRV
                     DAYQADQSAIRWLMQTTPGRYLDLGRGWMPNSYASAVRQGDTIWLNWVNTVYREAMLG
                     VDFDIMKASYKKWFGIDLETPRVGFPQEFS"
     gene            complement(2233..2760)
                     /locus_tag="P5558_22910"
     CDS             complement(2233..2760)
                     /locus_tag="P5558_22910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27234.1"
                     /translation="MPGRKPAAGSLGNFAFWSAPAPGKFRKIDKNIFFFKILRAPGFV
                     GARPGSFVDSLGSFVDSLGSIVDSAGSFVGRCCIFRDIPGPDVAIDATIRHRMSRYTR
                     QPKEKCRVYRDNPGILAHRLQQAAGMADTVPVDGPDRATGHRDGGPLDRRPPGAGIAT
                     IRLRRADRLDSAWKA"
     gene            2790..3470
                     /locus_tag="P5558_22915"
     CDS             2790..3470
                     /locus_tag="P5558_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27235.1"
                     /translation="MMIETTPTLGKLLAARLEAELGDPEVAGALAAILAEDALAWRAS
                     VIGLASVKTIIGFTFGNRMLANGNREPGPVNEALADLALRLHEATGAPIRAQWEVAEP
                     LAGRLPAGMVTPIHPTRDARAEPLYLSTGGVIEAIIARAGGVEAVGPVAVVAQRDHAW
                     RCVALCRRHGLRAGVVEGWELPAGYDAASGQPWTRSRLAYLLHDLHCRALDRRDALLA
                     GNGPARLA"
BASE COUNT          636 a         1197 c         1243 g          523 t
ORIGIN      
        1 cctcgagatc gtcaaggtcg tgcccttgac gatctcgagg gcggtgttga cccagggcgg
       61 caaggcccgg cgcaccgcct gcggcaggat gatccagcgc agccgctgga agaagcgcat
      121 gccgatcgcc atgccggcct cgtgctggcc ctgcgggatc gactgcaccg ccccccgcac
      181 gttctccgcc acgtgcgaga cgcagaagag cgagagggcg atgaccccgg cccagtaggg
      241 cttcaccccg atcttgagga tggcgagccc gtaataggtg aagaggatca gcaccaggac
      301 cgggatgccg cgcatcacgt cgacatagag ccgggcgagg agccgcaacg gcagcatgcc
      361 gtaggtcagg atcaggccga cgacgaagcc caggaccgtg ccgatgacga tggtcatgat
      421 cgagctcgag atcgtcacca tgagcccctc gagcagcgac cagcgcgccg tccaggcttg
      481 gcagaagaag gtgctgcagt ccgggtccat gtcagcgccc gtccatgatc agcgcaccac
      541 cgcgtagcgc ttctcgacct gccggagcgc gatcgcgatc gcataggccg tcaccagata
      601 gagagcactg gcggtgagcc agcattcgat cacccggtag gtgttggcgt tgatctgccg
      661 ggccgtgaag gtcagctccg gcacggcgat ggccgccgcc agcgaggtgt ccttgaagag
      721 cgagatcagg ttgttggaga ccgccggcag ggtgatgcgg aacatcaccg gcagcacgac
      781 atggcgctgc cgctgccagg gccgaaggcc gatcgccttc gccgcctcgc cataggcgcg
      841 cggcatggag gcgaggccgg cgcggaagac ctcgcacatg taggccgcgg catagcccga
      901 gagtgtcagg atgaaggact ggatcttgtc caggttgtag atgccgatct ccggcaggcc
      961 gaaatagacg aagaagatca ggagcaggag cggcgtgttg cggatgaact cgacatacgc
     1021 ccaggccggc ctggagatcc aggggctcgg cgacaaccgc gcataggcga ggaacaggcc
     1081 gatgatcgtg ccgatcacga gcgacacgac ggccaggccc agcccgagcg cgagggcgcc
     1141 gaggaacgca tcccaattgc gccagatcac ggcccaattg aagttgtagg cgatcatcgg
     1201 cgcagcctgc gctcgggtcc gggcactgcc aggaggatca ggagaattcc tgcgggaagc
     1261 cgacccgcgg tgtctcgaga tcgatgccga accacttctt gtaggacgcc ttcatgatgt
     1321 cgaagtcgac gcccagcatg gcctcgcgat agaccgtgtt cacccagttc agccagatcg
     1381 tgtcgccctg gcggaccgcc gaggcgtagg agttgggcat ccagccccgg ccgaggtcga
     1441 gatagcggcc gggcgtcgtc tgcatcagcc agcggatcgc cgactggtcg gcctggtagg
     1501 cgtccacccg ccgtgcgttc agggcctgca gcgtcgagtc cgggctgtcg aactggtcga
     1561 ccgtcgcctc gggcaggccg gcatggatcc agtcctcgat gaagacattc tgcatcgccc
     1621 cgacgcgcgc atccgcgccg gcggcgagca tgtcctcgaa ggtcgtgaac cgggcgcctt
     1681 cggccatcac cagaaggccg atgccctcgc ggtagtaggg cacggtgaag gcgcactgct
     1741 gcgcccgtcc cggggtgatc gtcatccact ggcagatcac gtcgacctgg tcggtgatca
     1801 ggctcgggat gcgcgcgtcc gagccctgca ggacgaactc gaccttggtc gggtcgtcga
     1861 acaggttctt ggcgatcaga tgcgccatgt cgatgtccat gccgaccagc gtgccggtct
     1921 cgtcctcgaa atgccagggc gggttggtgc tgccggtgcc gacgatcaga tgcccgcggt
     1981 ccaggacctg ctgcagcttg gaggcggatg gatcgacggg ctgggcctgc gccgcccggg
     2041 gcgccacgag gccggcgacc ccgccggcca gcccggcggc accaccagcc accgcgccca
     2101 tcccgagcag cccgcgccgc ttcagcatcg agaacgccag atcccgcaac tcacccatgc
     2161 ctaacccctt tcattgctcg accggcgctc gccgccggcc cgctcgctag cttcgagctt
     2221 gcaactatcg tgctaggctt tccacgcgct gtccagcctg tcggcccgcc gcaaccggat
     2281 cgtggcgatg ccggcgcccg ggggacgccg atcgagcggg ccgccgtcac ggtgacccgt
     2341 cgctcggtcc ggcccgtcca ccggcaccgt gtcggccatc ccggccgcct gttgcaggcg
     2401 atgcgccaat atccccgggt tgtcgcggta tacgcgacat ttctccttgg gttgtcgcgt
     2461 ataccgcgac atgcggtgac gaattgttgc atctatcgcg acatccggcc cggggatgtc
     2521 gcggaaaatg caacatcggc cgacgaacga acccgcggaa tcgacgatcg aacccagcga
     2581 atcgacgaag gaacccaacg aatcgacgaa cgaacccggc cgggcgccaa cgaacccggg
     2641 agcgcgcaag atcttgaaaa agaatatgtt tttgtctatt ttccggaact ttccgggggc
     2701 gggcgccgac cagaaggcaa aattgcccaa cgaacccgca gcgggcttgc gccccggcac
     2761 gaaataggct ataaatccta gaaaggcgca tgatgatcga aacgacgccg accctcggaa
     2821 agctgctcgc cgcccggctc gaggccgagc tcggcgaccc ggaagtggcc ggcgccctgg
     2881 cggcgatcct ggcggaggat gccctggcct ggcgggcgag tgtgatcggg ctggcgtcgg
     2941 tgaagacgat catcggcttc accttcggca accggatgct ggccaacggc aaccgcgagc
     3001 cggggccggt caacgaggcg ctggccgacc tggcgctgcg cctgcacgag gcgacggggg
     3061 caccgatccg ggcgcagtgg gaggtggccg agccgctcgc cgggcggctg ccggccggca
     3121 tggtcacccc gatccacccc acccgcgatg cccgggcgga gccgctctac ctgagcaccg
     3181 gcggggtgat cgaggcgatc atcgcccggg ccggcggggt cgaggcggtt gggcctgtgg
     3241 cggtcgtcgc ccagcgcgat cacgcctggc gctgcgtcgc cctctgccgg cggcacggcc
     3301 tcagggccgg tgtggtcgag ggctgggagc tgccggccgg gtacgatgcc gcctccggcc
     3361 agccctggac ccgcagccgg ctcgcctatc tgctgcacga cctgcactgt cgcgcactcg
     3421 atcgccgcga cgccctcctc gccgggaacg gccccgcaag gctggcctga aaggccatcc
     3481 ggcgctaaca agagcaggtc cgaggagcga gagggaaggc agttcgatgc aggtcaaggc
     3541 ggcggtggcg ttcgcggcgg gcaagccgct cgaggtgacg acggtcgagc tcgagggcc
//
LOCUS       DAOTFI010000355         3595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_30661_length_3595_cov_5.781921, whole genome shotgun sequence.
ACCESSION   DAOTFI010000355 DAOTFI010000000
VERSION     DAOTFI010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30661_length_3595_cov_5.781921"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="P5558_22920"
     CDS             complement(<1..394)
                     /locus_tag="P5558_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY27236.1"
                     /translation="MGIETATILIIAVMLGFMLLGVPLAWTTMALAVGCTLLWLGPAG
                     LPLVSSRVYGFINEYVLVAVPLFVFMACVLERSGVAHDLYDAMRVFAGRLPGGVAVQT
                     TLVAIVMAAMTGIIGGEIVLLGLIALPQM"
     gene            complement(407..916)
                     /locus_tag="P5558_22925"
     CDS             complement(407..916)
                     /locus_tag="P5558_22925"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRY27237.1"
                     /translation="MQAIETFNLWLGRQVGWFYLGAVVFTFYEVLARYIFNAPTTWAF
                     EVTIFLCASGYMLSGANVTAEEKHIAITSVVEIAGPGARWCMRLVALVVGLFGMAGLI
                     WGSWTSGIRALTIWERTGSAYNSPTPALIKPIITAAGILVLLQLALQLARHLRAGPRG
                     PRTAAPPPA"
     gene            complement(1011..2051)
                     /locus_tag="P5558_22930"
     CDS             complement(1011..2051)
                     /locus_tag="P5558_22930"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRY27238.1"
                     /translation="MKRWSTALFLGTAAVVALQAGTGAAAEVWKVQTSMTAGESYYQN
                     IEEFWLPKLAAMTNGELTIELTPVGSVVPYNETMDAIALGVLQGDITSTSYFAGRSKA
                     FSVMGDLIAGYDRPEQIALFCYQGGGREFLQELYDEFTDGQVKVVGCGTPAREAFVSK
                     VPIRGVADLEGKKVRSPEGLAAEVFKRAGAAPVGLPAAETYTSVDKGVVDAADFSSYT
                     MNDSLGFQQIAKYPIYPGIHSMPVLQFTVNKAAYDALDESLQVTIDVWYQAMVNDLRL
                     RNDITDQELVARDRADPNIEVIDWPQEERDKFRAIAQEAWAEYVAGDPLAEKALATNV
                     DFMKRVGLLPAD"
     gene            complement(2134..3150)
                     /locus_tag="P5558_22935"
     CDS             complement(2134..3150)
                     /locus_tag="P5558_22935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY27239.1"
                     /translation="MSRRPTIRDVARVAGVATVTVSRVANDPALVSPTTRARVEQAMR
                     DLGYVANAAARSMRTRLSRSIGCLVPDLVAYPNAAIAQAAERHLAAAGYSLLLTSSDY
                     DPAAEIRALEALRTRQVDGLLLYVSDETDPALNRALARLDMPLLVLDRTLAVDADVLL
                     SAHEPALQEAVRHLAGLGHRRLAIMLSGLRVRPVVERRRAFLRAVAEAGLDALAQRVV
                     EVPPEDHHRLRAAQDLLAGPAAPTAVLVEGSRQVRAVLAAARGLDRSIPHGLSLIALD
                     ASDIATVTTPELTTVTRDYERIGRVAAGLLLERLAAPGAPWRRVELESRVELRGSCGP
                     VWGD"
     gene            3304..>3595
                     /locus_tag="P5558_22940"
     CDS             3304..>3595
                     /locus_tag="P5558_22940"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY27240.1"
                     /translation="MQLSAEQIETYRRDGFIVVENVLSPAEVEELRQVTDAFVAKARF
                     VQTHDDVYDLEPSHSATEPRVRRIKTPHLQHDAYARIARHPAILAILQDLVHP"
BASE COUNT          568 a         1258 c         1263 g          506 t
ORIGIN      
        1 gcatctgcgg cagggcgatc aggccgagca gcacgatctc gccgccgatg atgccggtca
       61 tcgccgccat gacgatggcc accagcgtcg tctgcaccgc cacgccgccg ggcagccggc
      121 cggcgaagac gcgcatggcg tcgtagaggt cgtgcgccac cccggatcgt tccagcacgc
      181 aggccatgaa gacgaagagc ggcaccgcca ccagcacgta ctcgttgatg aagccgtaga
      241 cccggctcga gaccagcggc aggccggcgg ggccgagcca gagcagggtg cagccgacgg
      301 ccagcgccat ggtcgtccag gcgagcggca cgcccaggag catgaagccc agcatgacgg
      361 cgatgatcag gatggtggcg gtctcgatgc ccaagatcgg cttccgtcag gctggagggg
      421 gtgcggccgt tctcgggccc cggggccccg cccgcaggtg acgggcgagc tgcaaagcga
      481 gctgcagcag gacgaggatg ccggcggccg tgatgatcgg cttgatcagc gccggtgtcg
      541 gcgaattgta ggcgctgccg gtgcgctccc agatcgtcag cgcgcggatg cccgaggtcc
      601 aggagcccca gatcagcccg gccatgccga agagcccgac gacgagggcc acgagacgca
      661 tgcaccagcg ggccccgggc cccgcgatct cgaccacgct ggtgatcgcg atgtgctttt
      721 cctcggcggt gacgttggcg ccggagagca tgtagcccga ggcgcagaga aagatcgtga
      781 cctcgaacgc ccaggtggtg ggcgcattga agatgtagcg cgccagcacc tcgtagaagg
      841 tgaagacgac agcgccgaga tagaaccagc cgacctgcct gccgagccag aggttgaagg
      901 tctcgatcgc ctgcaagtgc ggcacccccc aggaatgtcg gcgagaacgt ggcggcggca
      961 ggccggcggc gggcgctccg agacgcccgc cgccggtccc gtgcgatcga tcagtcggcg
     1021 ggcaggagcc cgacccgctt catgaagtcg acattggtcg cgagcgcctt ctcggcgagc
     1081 ggatcgccgg cgacgtactc ggcccaggct tcctgggcga tggcgcggaa cttgtcgcgc
     1141 tcctcctggg gccagtcgat gacctcgatg ttcggatcgg cgcggtcgcg ggcgacgagc
     1201 tcctgatcgg tgatgtcgtt ccgcaggcgc aggtcgttga ccatcgcctg gtaccagacg
     1261 tcgatcgtca cctgcaggct ctcgtcgagg gcgtcatagg ccgccttgtt caccgtgaac
     1321 tgcaagaccg gcatcgaatg gatgcctgga tagatcgggt acttggcgat ctgctggaag
     1381 cccaggctgt cgttcatggt gtaggagctg aagtccgccg cgtcgaccac acccttgtcg
     1441 acgctggtgt aggtctcggc cgccggcagg cccaccggcg ctgcgccggc gcgcttgaag
     1501 acctcggcgg ccagaccctc cggcgagcgg accttcttcc cctcgaggtc ggcgacaccg
     1561 cggatcggca ccttcgagac gaaggcctcg cgcgccgggg tgccgcagcc gacgaccttg
     1621 acctggccgt ccgtgaactc gtcgtagagc tcctgcagga actcgcggcc gccgccctga
     1681 tagcagaaga gggcgatctg ctcgggccgg tcatagccgg cgatgaggtc gcccatcacc
     1741 gagaaggcct tgctgcggcc ggcgaaatac gaggtcgagg tgatgtcgcc ctgcagcacg
     1801 ccgagggcga tggcgtccat cgtctcgttg tacggcacga ccgagccgac cggcgtcagc
     1861 tcgatggtca gctcgccgtt ggtcatcgcc gcgagcttgg gcagccagaa ctcctcgatg
     1921 ttctgatagt agctctcgcc ggcggtcatc gaggtctgca ccttccagac ctccgccgcg
     1981 gcaccggtgc ccgcctgcag tgccaccacc gctgcggtgc ccaggaacag ggccgtgctc
     2041 caccgcttca tcatctcgcc tcccgctttc tcatgtgtcg cccgttggcg tcgcggcgca
     2101 acgcttggca tgaagctcgt gaaaccgtca acatcaatcg ccccacaccg gcccgcagga
     2161 gccgcgcagc tcgacccgac tctccagctc gacccggcgc cagggtgccc cgggtgctgc
     2221 cagccgctcg agcaggaggc ccgccgccac cctgccgatc ctctcgtagt cgcgggtcac
     2281 cgtggtgagc tcgggcgtgg tcacggtggc gatgtccgag gcatcgaggg cgatcagcga
     2341 tagcccatgc gggatcgacc ggtcgagccc gcgcgcggcg gcgagcacgg cccggacctg
     2401 ccggctgccc tcgacgagga ccgccgtggg tgccgccggc ccggccagca ggtcctgcgc
     2461 cgcgcgcagc cggtggtgat cctcgggcgg cacctccacc acgcgctggg ccagggcatc
     2521 gagcccggcc tcggcgaccg ccctgaggaa ggcccgccgc cgctcgacca ccggccgcac
     2581 ccgcaggccc gagagcatga tggcgaggcg ccggtggccg aggccggcga ggtggcggac
     2641 cgcctcctga agcgccggct cgtgcgccga gagaaggacg tcggcatcca cggccagcgt
     2701 gcggtcgagc accaggagcg gcatgtccag ccgggcgaga gcccggttca gcgccgggtc
     2761 ggtctcgtcg gagacgtaga gcaggaggcc gtcgacctgg cgcgtgcgca gcgcctcgag
     2821 cgcccggatt tcggccgccg ggtcgtagtc gctgctggtc agcaggagcg agtagccggc
     2881 cgccgccagg tgccgctcgg ccgcctgggc gatcgcggcg ttggggtagg cgacgaggtc
     2941 gggcacgagg cagccgatgc tgcgcgacag ccgggtgcgc atcgagcgtg ccgccgcgtt
     3001 ggcgacatag cccaggtccc gcatcgcctg ctcgacgcgg gcccgggtgg tggggctcac
     3061 cagcgccgga tcgttggcga cgcgcgagac ggtgacggtc gcgacgccgg cgacacgcgc
     3121 cacgtcgcgg atcgtcggcc gccggctcac actcgtccct ttccgctcat gacgacgcca
     3181 ccacgacgcg cggcggcgtc gctgtcaagc gggttggcga ggcaggcggc acttcgatgt
     3241 tgacgttttc atccaccgtc ggcacgttcc ggcgcaaact cagaccatcc aggggagcgg
     3301 gccatgcaac tgagcgccga gcagatcgaa acctatcgcc gggacggctt catcgtcgtc
     3361 gagaacgtcc tctcgcccgc cgaggtcgag gagctgcgcc aggtcaccga cgcgttcgtc
     3421 gccaaggccc gcttcgtcca gacccacgac gacgtctacg acctcgagcc cagtcacagc
     3481 gccaccgagc cccgggtgcg ccggatcaag acgccgcatc tccagcacga cgcctacgcc
     3541 aggatcgcgc ggcacccggc gatcctggcc atcctccagg acctcgtcca cccgg
//
LOCUS       DAOTFI010000356         3581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_30822_length_3581_cov_5.448100, whole genome shotgun sequence.
ACCESSION   DAOTFI010000356 DAOTFI010000000
VERSION     DAOTFI010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30822_length_3581_cov_5.448100"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="P5558_22945"
     CDS             <1..275
                     /locus_tag="P5558_22945"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27241.1"
                     /translation="APQWDPESETVKKCNMCIDELEAGRKPYCVMACMMRVLDIGPID
                     QLRAGTYETKARGAADEIVGQVKTMADPELTRPSIVFVPHSKGRVG"
     gene            277..996
                     /locus_tag="P5558_22950"
     CDS             277..996
                     /locus_tag="P5558_22950"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HRY27242.1"
                     /translation="MSLANPVPWSEVAEDLGMLALLHDREPTAALLDDLRRHPIQGWL
                     GLHLGGADGARLLDLALADLPAVIDRAVVDELAADYVDIYLVHGLHAAPAESPWLDRE
                     HLVCQEPMFEVREWYAHYGLAAENWRRRSDDHLVLQLGFLAELARRGTPASILDIGRF
                     LDRHLLRWLPDFAQRVVQRARTPYFAGLAQLTVDYAEVLRLLVVEVTGEPRRRIEADP
                     KRAAAAPVAPQPYLPGAQPGW"
     gene            999..1961
                     /locus_tag="P5558_22955"
     CDS             999..1961
                     /locus_tag="P5558_22955"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY27243.1"
                     /translation="MPSVPTVDMDGCVRGMSPLASCRACVDSCPQGVWTLLDRGPALD
                     AGRCDGCGHCQAACPQQAIGLAAQPQLREDRRGIFAVAICEPAAAAADGAWTLPCLHM
                     LGWRQLVGLRRQGVDRLYACRLSCDGCPRAATTRLDEHLAVAKRWWAAGADPGLEVVF
                     LSRQGFADRLRSSEPAHPRGTSRRRLLTRWLAPDEPPARQGLLEVFVPEIDPASCSGC
                     DACARLCPTDALAIRERGEGLAYVVDGSHCTGCNLCVDVCEDEAVSVRQWAVAKRVEI
                     GLRERCCPSCKVAFHRPEASPDDDVLCAICRLKGVRSKRPVVLA"
     gene            1958..2431
                     /locus_tag="P5558_22960"
     CDS             1958..2431
                     /locus_tag="P5558_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27244.1"
                     /translation="MSAGTQPEGHWLSGLQRHLATTAPAATAFVLPAYRPDLVLAAAE
                     MLGFVHVDFRKDYMRDGGGDPALQPLDLIERAIGDHAGAAGLVLQNVEALLATRAASV
                     RQAWLAGFLGSPRPFSVLLPVVLFAADLPRTASVVTIDAEAIPPDSLLMRLWAQR"
     gene            2428..>3581
                     /locus_tag="P5558_22965"
     CDS             2428..>3581
                     /locus_tag="P5558_22965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRY27245.1"
                     /translation="MSGIASGLLGWLDVSLRRKLAGVLLAALAAASLVFLVFLVTLYR
                     ERLVSERQTAAAQVTQLLQATLENAMLKRDIDGLRDVVARLATQSDVERVMILEPSGE
                     VRFASSEADLGRVLDLAGGALCPGCALAPGETRAMTAVVQSADGDEMIRSVQPVRNRE
                     PCQGCHGPIAANPVNGILVVDYLAGGLQGSMLRAGLLMSGLGLVAAGLAVVGVWLALE
                     AWVLRPLGRLGSVGARLGAGELSARVGSTGRDEIAVLGRRFDAMAGDIERYMEVLASR
                     ERFLQALLDAIPDGIRVIGPDFGVEMVNRAFLELHGSSDLQVVGAPCYRSSHGRDSPC
                     PTPLVTCPLQELTRSGASLTCRHRHVRADGSELFVEVSAAAFETRRDGRT"
BASE COUNT          436 a         1254 c         1338 g          553 t
ORIGIN      
        1 gcgcgccgca atgggacccc gaaagcgaga ccgtcaagaa gtgcaacatg tgcatcgacg
       61 agctggaggc cggccgcaag ccctactgtg tcatggcgtg catgatgcgc gtgctcgaca
      121 tcggcccgat cgaccagctg cgcgcgggca cctacgagac caaggcgcgc ggcgccgccg
      181 acgagatcgt gggtcaggtc aagaccatgg ccgatcccga gctgacccgg ccgtcgatcg
      241 tgttcgtgcc gcattccaag ggcagggtgg ggtaggatga gcctcgccaa ccctgtgccc
      301 tggagcgagg tcgccgagga tctcgggatg ctggcgctgc tgcacgatcg cgagccgacg
      361 gcggccctgc tcgacgacct gcggcgccac ccgatccagg gctggctcgg ccttcatctg
      421 ggcggggccg acggcgccag gctgctcgac ctggcgctgg ccgacctgcc ggcggtgatc
      481 gatcgggcgg tggtcgacga gctggcggcg gattacgtcg acatttatct cgtccacggg
      541 ctgcatgcgg cgcccgccga atcgccctgg ctcgaccgcg agcacctggt ctgccaggag
      601 ccgatgttcg aggtccgcga gtggtacgcg cattacggcc tggcggcaga gaactggcgc
      661 cggcgctcgg acgatcacct ggtgctgcag ctcggcttcc tcgccgagct ggcccggcgc
      721 ggcacgccgg cgagcatcct cgatatcggc cgcttcctcg accgccatct gttgcgctgg
      781 ctgccggatt tcgcccagcg tgtggtgcag cgcgcccgca ccccgtattt cgccggtctc
      841 gcccagctca ccgtcgacta tgccgaggtg ctgcgcctgc tggtcgtcga ggtcaccggc
      901 gagccgcgcc gccggatcga ggccgacccg aagcgtgcgg cggcagcgcc cgtggcgccg
      961 cagccctatc tgccgggcgc ccagccgggg tggtgatcat gccgtcggtg ccgacggtcg
     1021 acatggacgg ctgcgtgcgc ggcatgagcc cactggcgag ctgccgcgcc tgcgtcgaca
     1081 gttgcccgca aggggtttgg acgctgctcg atcggggccc ggcgctcgat gccgggcgct
     1141 gcgacggctg cgggcactgc caggcggcgt gcccgcagca ggcgatcggc ctggcggcgc
     1201 agccgcagct ccgcgaggac cggcgcggca tcttcgccgt ggcgatctgc gagccggcgg
     1261 cggcggcggc cgacggcgcc tggacgctgc cctgcctgca catgctcggc tggcgccagc
     1321 tcgtcggcct gcgccgccag ggggtcgacc ggctctacgc ctgtcggctc tcctgcgacg
     1381 gctgcccgcg cgcggcgacc acccggctcg acgagcatct ggccgtggcg aagcggtggt
     1441 gggcggcggg cgccgatccc ggcctcgagg tggtctttct gagccggcag ggcttcgccg
     1501 accgcctgcg ctcgagcgag ccggctcacc cgcgagggac cagccggcga cgcctcctga
     1561 cgcgctggct cgcccccgac gagccgccgg cacgccaggg cctgctcgag gtgttcgtgc
     1621 ccgagatcga tccggcgagc tgcagcggct gcgacgcgtg cgcgcgcctg tgcccgaccg
     1681 acgccctcgc catccgcgag cgtggggagg ggctcgccta cgtggtcgac gggtcgcatt
     1741 gcaccggctg caatctatgc gtcgatgtct gcgaggacga ggcggtcagc gtgcgccagt
     1801 gggcggtcgc gaaacgggtg gagatcggct tgcgggaacg ctgttgccca tcgtgcaagg
     1861 tggcgtttca tcgacccgag gcgtcgccgg acgacgacgt gctctgtgcc atttgccggc
     1921 tgaagggggt ccgcagcaag cggcccgtgg tgctggcgtg agcgcgggca cgcagccgga
     1981 ggggcactgg ctgagcgggt tgcagcgcca cctggcgacg accgcgccgg cggcgacggc
     2041 tttcgtgctg ccggcctatc gccccgatct ggtgttggcg gcagccgaga tgctcggctt
     2101 cgtccatgtc gattttcgca aggactacat gcgcgacggt ggcggcgatc cggccctgca
     2161 gccgctcgac ctgatcgagc gggcgatcgg cgaccatgcc ggtgctgccg gcctcgtgct
     2221 gcagaacgtc gaggcactgc tcgccactcg tgcggcgagc gtgcgccagg cgtggctcgc
     2281 gggtttcctg ggctcgccgc ggccgttttc ggtgctgctg ccggtcgtgc tgttcgccgc
     2341 cgacctgcca aggacggcga gcgtggtcac gatcgacgcc gaggcgatcc cgccggacag
     2401 cctcctgatg cggctgtggg cccagcgatg agcgggatcg ccagcggctt gctcggctgg
     2461 ctcgacgtct cgctccgccg caagctcgcc ggcgtgctcc tggcggcgct ggctgcggcc
     2521 tcgctggtct ttctggtctt tctcgtcacg ctctaccgcg agcggctggt cagcgagcgc
     2581 cagaccgctg cggcgcaggt gacgcagctt ctccaggcga ccctcgagaa cgcgatgctc
     2641 aagcgggaca tcgacggcct gcgcgatgtc gtcgcacggt tggcgacgca gagcgacgtc
     2701 gagcgggtga tgatcctcga gccttccggc gaggtgcgct tcgcctcgtc cgaggcggat
     2761 ctcggccgcg tcctcgacct ggccggcggc gcgctctgtc ccggctgcgc cctggcgccc
     2821 ggcgagacgc gcgccatgac cgccgtggtg cagagcgccg atggcgacga gatgatccgc
     2881 agcgtgcagc cggtgcgcaa ccgcgagccc tgccagggct gccacggacc gatcgccgcc
     2941 aacccggtca acggcatcct ggtcgtggac tatctggccg gcgggctgca ggggtcgatg
     3001 ctgcgggccg ggctcctgat gagcggcctc ggcctcgtcg ccgccggcct cgccgtggtc
     3061 ggggtctggc tggcgctcga ggcctgggtg ctgcgtccgc tcggccgcct cggcagcgtc
     3121 ggggcgcggc tcggggcggg cgagctgtcg gcgcgggtcg ggtcgaccgg gcgcgacgag
     3181 atcgccgtgc tcggccgtcg cttcgacgcc atggccggcg acatcgagcg ctacatggag
     3241 gtcctggcct cgcgggagag gttcctgcag gcgctgctcg atgccatccc ggacgggatc
     3301 cgcgtgatcg gcccggactt cggggtcgag atggtcaacc gtgccttcct ggagctgcac
     3361 ggatcgagcg acctgcaagt ggtcggggcg ccgtgctacc ggtccagcca tggtcgcgac
     3421 agcccgtgcc cgacaccgct cgtcacctgc ccgctgcagg agctgacgag gagcggcgcc
     3481 tcgctcacct gtcgccaccg gcatgtccgg gccgacggca gcgagctctt cgtcgaggtc
     3541 tcggccgccg cgttcgagac ccgccgcgat gggcgaacgg a
//
LOCUS       DAOTFI010000357         3573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_30934_length_3573_cov_8.019613, whole genome shotgun sequence.
ACCESSION   DAOTFI010000357 DAOTFI010000000
VERSION     DAOTFI010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30934_length_3573_cov_8.019613"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1037
                     /locus_tag="P5558_22970"
     CDS             3..1037
                     /locus_tag="P5558_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRY27246.1"
                     /translation="MRHATPRTVGAADATLHLALTGSRFLPHCSLPAALANGVDGLPV
                     DDLLAFHLVFGKSVPDISLNAVANLGYAEGRFHRLVRPGDTLAATSTVIGLKETSSGR
                     TGIVYVRTVGIDQDGRPVLEFCRWVMVRKRDAASPAPETSVPTLAPAVDAATLAVPAG
                     LGRLVLDPVASGSALTFEGYAKGERIDHVDGMGIMESEHRLATRLYQNTARVHFNDHA
                     ERAGRLGAVIVYGGVVISLARALSFNGLGNLWHLLAINAGTHANPALAGDTVFAWSEV
                     LDKAPLAERDDCGALRLRLVATKNRPCHDYPLRDTDGRYLPDVLLDLDYWGLMPTRAA
                     LSAGSGGRSG"
     gene            complement(1006..1584)
                     /locus_tag="P5558_22975"
     CDS             complement(1006..1584)
                     /locus_tag="P5558_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRY27247.1"
                     /translation="MAAAGNLLPRDGILRLVGEAVPEPDEVMARLLAEIEWRQDIAMI
                     VGRPVKLPRLTAWHGDAGYRYSGIDNPPKAWTPLLLELRSVAERVAGSAFNSVLLNLY
                     RDGSDGMGWHADDERPLGQDPVIASLSFGATRRFRLKHRTEPGNSLTLELPPGSCLVM
                     AGALQHHWRHALPKTRRAVGPRVNLTFRLIRR"
     gene            complement(1590..2918)
                     /locus_tag="P5558_22980"
     CDS             complement(1590..2918)
                     /locus_tag="P5558_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRY27248.1"
                     /translation="MSHVFYRHLRETYPTAAKGDGVHVVDTDGKRYLDASGGAAVSCL
                     GHSHPGVIAAIKAQLDAIPYAHTGFFTSEPAEALADLLVEKAPPGIDRVYFVSGGSEA
                     VEAALKLARQYFLEIGEPQRKRFVARRQSYHGNTLGALAVGGNALRRAQFEPILIDAD
                     HLTPCYAYREKAAEETEEAFGQRLAAELEAYLERVGPETVIAFVAETLVGATLGAVPP
                     VPGYFRRVREILDRHGILLVLDEVMCGMGRTGTLFASEQEGITPDLVCVAKGLGAGYQ
                     PIGATLVHGRIAAAIERGSGFFQHGHTYMAHPTACAAGLAVQRAIEDEGLLERVERMG
                     ELLERSLIERFGNHRHVGDIRGRGLFRGLELVADRATRAPFDPAAALNRRIKKAAMQA
                     GLMCYPMGGTIDGRSGDHILIAPPFIVDESHIAELTDRLGLAVDEAIASL"
     gene            complement(2946..3524)
                     /locus_tag="P5558_22985"
     CDS             complement(2946..3524)
                     /locus_tag="P5558_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27249.1"
                     /translation="MSIRTALGLATVLLIAACTGPEIREPVEVTSAKPPLIFTVAEAV
                     ITDTSQLPETATFRDRQWSERLSDATEAFLLDRLQTGSGQGWLQVTIEQASLVEADLP
                     TASGIGAALRREPDRVLEAQLAVRIAVMGPDGLEQVYADTEVSRRRPILRNTSVMARD
                     AEAQRLIGDFLEQFDDALTQNARQNLGAWLVY"
BASE COUNT          516 a         1269 c         1262 g          526 t
ORIGIN      
        1 tgatccgcca cgccacgccg cgcacggtcg gcgccgccga tgccacgctc catctggcgc
       61 tcaccggctc gcgcttcctc ccccattgtt cgctgcccgc ggcccttgcc aatggcgtgg
      121 acggcctgcc ggtcgacgat ctcctcgcct tccacctcgt cttcggcaag agcgtgccgg
      181 acatctcgct caacgcggtc gccaatctcg gctacgcgga agggcgtttc catcgcctcg
      241 tccggccggg cgacacgctc gccgcgacat cgacggtgat cggcctgaag gagacctcga
      301 gcggcaggac gggcatcgtc tacgtgcgga cggtcggcat cgaccaggac ggccgcccgg
      361 tgctcgagtt ctgccgctgg gtcatggtgc gcaagcggga cgcggcgagc ccggcgcccg
      421 aaacctcggt gccgaccctg gcacctgcgg tcgatgccgc gaccctcgcc gtcccggcgg
      481 ggctcggtcg cctcgtgctg gatccggtgg ccagcggctc ggccctcacc ttcgagggct
      541 acgcgaaggg cgagcgcatc gaccatgtcg acggcatggg catcatggag agcgagcacc
      601 gtctcgccac ccggctctac cagaacacgg cgcgcgtgca tttcaacgac cacgccgagc
      661 gggccggccg gctcggcgcc gtcatcgtct atggcggggt ggtcatcagc ctcgcccggg
      721 ccctctcgtt caacggcctc ggcaatctct ggcacctgct cgcgatcaac gccggcaccc
      781 acgccaaccc ggccctggcg ggcgacaccg tcttcgcctg gtcggaggtg ctggacaagg
      841 cgccgctcgc cgagcgcgac gattgcggtg cgctcaggct ccggctcgtc gccacgaaga
      901 accgcccttg ccacgactac ccgctgcgcg acaccgacgg ccgctacctg ccggacgtgc
      961 tgctcgatct cgactactgg gggctgatgc cgacccgggc cgccctcagc gccggatcag
     1021 gcggaaggtc aggttgaccc tgggccccac ggcccgtcgc gtcttcggca gcgcatgccg
     1081 ccagtgatgc tgcaaggcgc cggccatgac gaggcacgag ccgggcggca gctcgagcgt
     1141 gagggaattg ccgggctccg tccggtgctt gagccggaac cggcgggtcg cgccgaagga
     1201 gaggctggcg atcaccgggt cttggccgag cggccgctcg tcgtccgcat gccagcccat
     1261 gccgtcgctg ccgtcgcggt agaggttgag cagcacgctg ttgaaggccg aaccggcgac
     1321 ccgctccgca accgaccgaa gctcgagcag gagcggcgtc cacgccttgg gcgggttgtc
     1381 gatgccgctg tagcggtagc cggcgtcgcc atgccaggcg gtcaggcgcg gcagcttcac
     1441 cggccgcccg acgatcatgg cgatgtcctg gcgccactcg atctccgcca ggagccgcgc
     1501 catgacctca tccggctcgg gcaccgcctc gcccacgaga cgcagaatcc cgtcgcgggg
     1561 gaggagattg ccggcagcgg ccatgcagct cagagcgacg ctatcgcctc gtcgacggcc
     1621 aggcccagcc ggtccgtgag ctcggcgatg tggctctcgt cgacgatgaa gggcggggcg
     1681 atcaggatgt gatcgccgct gcggccgtcg atcgtgccgc ccatcgggta gcacatcagg
     1741 cccgcctgca tcgccgcctt cttgatccgc cggttgagcg ccgccgcggg atcgaagggt
     1801 gccctggtgg cgcggtcggc aaccagctcg aggccgcgga agaggccgcg cccccgaatg
     1861 tcgccgacat ggcgatggtt gccgaagcgc tcgatcaggc ttcgctcgag cagctcgccc
     1921 atcctctcga cccgctcgag caggccctcg tcctcgatcg cccgctgcac ggcgaggccg
     1981 gcggcgcagg ccgtcggatg ggccatgtag gtgtggccat gctggaagaa gccgctgcca
     2041 cgctcgatcg ccgccgcgat ccgcccgtgc acgagcgtcg ccccgatcgg ctggtagccg
     2101 gcgccgagcc ccttggcgac gcagacgagg tcaggggtaa tgccctcctg ctccgaggcg
     2161 aagagcgtgc cggtgcggcc catgccgcac atcacctcgt cgaggacgag gaggatgccg
     2221 tggcgatcga ggatctcgcg gacccggcgg aaatagccgg gaacgggggg cacggcaccc
     2281 agcgtggcgc cgaccaacgt ctcggcgacg aaggcgatca ccgtctcggg cccgacgcgc
     2341 tccagatagg cctcgagctc cgccgcgagg cgctggccga aggcctcctc cgtctcctcg
     2401 gccgccttct cccgataggc gtagcagggt gtgagatgat cggcgtcgat caggatcggc
     2461 tcgaactgcg cccggcgcag ggcattgccg ccgacggcga gggcacccag cgtgttgccg
     2521 tgatagctct gccggcgcgc gacgaagcgc ttgcgctgcg gctcgccgat ctcgaggaaa
     2581 tactggcggg ccagcttgag cgccgcctcc accgcctccg acccgcccga gacgaaatag
     2641 acgcggtcga tgccgggcgg cgccttctcg accaggagat cggccagcgc ctcggccggc
     2701 tcgctcgtga agaagccggt atgggcatag gggatggcgt cgagctgcgc cttgatcgcg
     2761 gcgatcacgc ccgggtggct gtgaccgagg caggagacgg cggcaccgcc cgaggcatcg
     2821 agatagcgct tgccgtccgt gtcgacgaca tgaaccccgt cacccttcgc cgccgtcggg
     2881 taggtctcgc gcagatgacg atagaagacg tggctcacgc tgctgctccg tcgtcgggcc
     2941 ggcgctcagt agacgagcca ggcgccgaga ttctgccgcg cgttctgggt cagcgcgtcg
     3001 tcgaactgct cgaggaaatc gccgatcagc cgctgggcct cggcgtcgcg tgccatgacc
     3061 gaggtgttgc gcaggatcgg ccggcgccgg ctcacctcgg tgtcggcgta gacctgctcg
     3121 agcccgtcgg ggcccatgac cgcgatgcgc acggcgagct gtgcctcgag cacccggtcg
     3181 ggctcccgcc gcagggcggc gccgatgccg gacgcggtcg gcagatccgc ctcgacgagg
     3241 ctcgcctgct cgatggtcac ctgcagccag ccctggcccg agccggtctg gagccggtcg
     3301 agcaggaagg cctcggtcgc gtccgagagc cgctcgctcc attgccggtc gcggaaggtg
     3361 gcggtctccg gcagctggct ggtgtcggtg atcacggcct cggccacggt gaagatcaag
     3421 ggcggcttgg cgctggtcac ctccaccggc tcgcggatct cggggccggt gcaggcggcg
     3481 atcaggagaa cggtggcaag gccgagcgcg gtgcggatgc tcaatgcagg ctgtcctcga
     3541 acagggtgaa gatatcggcg tcgctcagcc ggt
//
LOCUS       DAOTFI010000358         3494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_31827_length_3494_cov_5.592033, whole genome shotgun sequence.
ACCESSION   DAOTFI010000358 DAOTFI010000000
VERSION     DAOTFI010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31827_length_3494_cov_5.592033"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1496)
                     /locus_tag="P5558_22990"
     CDS             complement(87..1496)
                     /locus_tag="P5558_22990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY27250.1"
                     /translation="MTSHPRLPAATRSRCAILLATTLAGCALATPPDHETLLHAALPE
                     TSAIPVAWSTEGAPTSAVGGNWVASFDDPELARLVDEALRNNRDLQAAAARVEAALQS
                     VVIAGAPVLPAVGVEAGRHTSRNYGTNDTQTINGAELVVSWEIDLWGRLRSGEAAAFA
                     QAQAAADDAVYAQQSIAATVARVWVANIEVVQLTALAGESLDDYQRLVRLAQRKEAAG
                     QVSSFDVVQARSRASAARAAAARLESRANETIAGLEVLLGRYPSLGLKPADSFPHMPE
                     ALSAGLPLSLLDRRADVTAARNQVIAAFYDVEVAKLARLPGVSLAASAGRLNDPDLAL
                     LAMDSFSFAKIGVGLLQPIFEGGALQAQVAGMTATQAAATALYGQTVLDAFGEVETRL
                     ANEQLLREELAAWRAADKDAREAVRLGEANYVMGTIDMVTLLDLVEFAIGVRMDEIST
                     EAALLTNRIALYLALGESY"
     gene            complement(1505..2647)
                     /locus_tag="P5558_22995"
     CDS             complement(1505..2647)
                     /locus_tag="P5558_22995"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRY27251.1"
                     /translation="MLAVVAITFLYLLFIWVVFFKLKWLRLTPTWGVVSGFFLLHLVL
                     VPMVGMRFFAPLSTDLRVVRHTVQIVPRLPEPTLVEAILVAQNQPVEKGTPLFVLDKR
                     PYQYQLDQAKAALAAAKQNVLILQADVAVAAASVERAKAELVYKREQEQRFVSLAAQR
                     AAPKEDAQRWTAEVVAGEAAVAEAQANKERAEVAYASQIDGVNTVVAEAESQVAEAEY
                     YLEQTVIRAPEDGMIVNLQVQEGMVAGIVRAGAIASFIVERDPYLLATYRQENLKFVQ
                     EGQPVVVALNPYPGEHFSARVEEIWWASGRGQFLPSGELPVFPDQPSAEVRFPVKITM
                     DDPTVRLPIGAEGAALILTNDGPFTWLGQIALRTYTWSRWLYPLPF"
     gene            complement(2671..3087)
                     /locus_tag="P5558_23000"
     CDS             complement(2671..3087)
                     /locus_tag="P5558_23000"
                     /inference="COORDINATES: protein motif:HMM:NF023170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HRY27252.1"
                     /translation="MEWLGQHWSAFQASYYFWDVITLVCLALAVLGFLIGLVKLLGLP
                     GRIAVARNHPDAEAVYLMGWIGFAAIVPWIQALIWAFKPTDKIDIRRFPEAERIAEHK
                     ELAELAAYAYGRKLPPVDEDRQPDEGVPKEQKDKKP"
BASE COUNT          553 a         1183 c         1207 g          551 t
ORIGIN      
        1 aacccgagcg caaacgtcgc ggaatctgcg acaacccgag ccgcactgtc gcgtataacg
       61 cgacaatccg gggaagggcc ggctgttcag tagctctcgc cgagcgccag atagagcgcg
      121 atccggttgg tcaggagggc cgcctcggtg ctgatctcgt ccatcctgac gccgatggcg
      181 aactccacca gatcgagcag cgtcaccatg tcgatcgtgc ccatgacgta gttggcctcg
      241 ccgagccgca ccgcctccct ggcatccttg tccgcggctc gccaggctgc cagctcctcg
      301 cgcagcaact gctcgttggc gagtcgcgtc tcgacctcgc cgaaggcgtc gagcacggtc
      361 tgaccgtaga gggcggtggc cgccgcctgt gtggcggtca tgccggcgac ctgggcctgc
      421 agggcaccac cttcgaagat cggctgcagg aggccgacgc cgatcttcgc gaaggagaag
      481 gaatccatcg ccaggagcgc caggtcggga tcgttgagcc ggccggcgct ggcggcgagc
      541 gacacgccgg gcaaccgggc gagcttcgcc acctcgacgt cgtagaaggc cgcgatcacc
      601 tggttgcgcg ctgccgtcac gtccgcccgg cggtcgagca gcgagagcgg cagtccggcc
      661 gagagcgctt ccggcatgtg cgggaacgag tcggcgggct tcaggcccag gctcgggtag
      721 cggccgagca gcacctccaa tccggcgatg gtctcgttgg cgcgggattc gagccgggcc
      781 gcggctgccc gcgcggcgct ggcccgggat cgcgcctgga cgacatcgaa gctcgagacc
      841 tggcccgccg cttccttgcg ctgcgcgagc cggaccagcc tttggtagtc gtcgagcgac
      901 tccccggcga gggcggtgag ctgcacgacc tcgatgttgg cgacccagac gcgcgccacg
      961 gtcgccgcga tcgattgctg ggcatagacg gcatcgtccg cggcggcctg cgcctgggcg
     1021 aaggctgcgg cttcgcccga gcgcagcctg ccccagagat cgatttccca ggagaccacc
     1081 agctcggcgc cgttgatcgt ctgggtgtcg ttggtgccgt agttgcgcga ggtgtgcctg
     1141 ccggcctcga cgccgacggc gggcaggacg ggcgcgccgg cgatgacgac cgattgcagc
     1201 gccgcctcga cccgtgccgc ggctgcctgg aggtcgcggt tgttgcgcag cgcctcgtcc
     1261 accagccgcg cgagctccgg gtcgtcgaag ctcgcgaccc agttgccgcc aacggcgctc
     1321 gtcggcgcgc cctcggtcga ccaggcgacg gggatggcgc tcgtctccgg cagcgccgcg
     1381 tggagcaggg tctcgtggtc gggtggcgtc gccagggcgc agccggccag cgtcgtcgcg
     1441 agcaggatgg cacatctgct ccgagtggcg gccgggaggc gcgggtggga agtcatcggc
     1501 atcctcagaa cggcaacgga tagagccatc tggaccaggt gtaggtcctg agcgcgatct
     1561 gcccgagcca ggtgaaggga ccgtcattgg tcaggatcag ggcggcgccc tcggcgccga
     1621 tcggcaggcg gacggtcggg tcgtccatgg tgatcttgac cgggaaccga acctcggcgg
     1681 acggctgatc gggaaagacc ggcagctcgc ccgagggcag gaactggccc ctgccgctcg
     1741 cccaccagat ctcctcgacc cgggcggaga aatgctcgcc cgggtagggg ttcagggcga
     1801 ccaccaccgg ctggccctcc tgcacgaact tgaggttctc ctgtcggtag gtggccagga
     1861 ggtaggggtc gcgctcgacg atgaagctcg cgatcgcccc ggcccggacg atgccggcga
     1921 ccatgccctc ctggacctgc aggttgacga tcatgccgtc ctcgggggcg cggatcaccg
     1981 tctgctcaag gtagtattcg gcctccgcca cctgggattc ggcctcggcg accacggtgt
     2041 tcacgccgtc gatctgcgag gcgtaggcga cctcggcgcg ctccttgttc gcttgcgcct
     2101 cggcgacggc ggcctcgccc gccaccacct cggcggtcca gcgctgggcg tcctcctttg
     2161 gtgccgcgcg ctgggcagcg aggctgacga agcgctgctc ctgctcccgc ttgtagacga
     2221 gctcggcctt ggcgcgctcg acggaggccg ccgcaacggc gacgtcggcc tgcaggatga
     2281 ggacattctg cttcgccgcc gccagggccg ccttcgcctg gtcgagctgg tactggtagg
     2341 ggcgcttgtc gaggacgaag agcggggtgc ccttctccac cggctggttt tgcgcgacca
     2401 gaatcgcctc gacgagcgtg ggctccggca aacgcggcac gatctgcacg gtatgccgca
     2461 cgacgcgcag gtcggtcgaa agaggggcga agaagcgcat cccgaccatc ggcaccagca
     2521 cgagatgcag cagaaagaag cccgagacga ccccccatgt gggcgtcagg cgcagccatt
     2581 tgagcttgaa aaatacgacc caaatgaaaa gcaggtagag aaacgttatg gcgacaacgg
     2641 cgagcacgtc cgaggcctct ccgacctaga ttatggcttc ttgtctttct gctctttcgg
     2701 gacgccctcg tccggttgcc ggtcctcgtc gaccggcggc agcttcctgc cgtaggcata
     2761 ggcggcgagc tcggcgagct ccttatgttc cgcgatgcgc tcggcctcgg gaaaacgccg
     2821 gatgtcgatc ttgtcggtcg gcttgaaggc ccagatcagc gcctgaatcc aggggacgat
     2881 ggcggcgaag ccgatccagc ccatcaggta cacggcctcg gcatccgggt ggtttcgcgc
     2941 gacggcgatg cggccgggca ggccgagcag cttcacgagc ccgatcagga agcccaggac
     3001 agcgagggcg aggcagacga gcgtgatcac gtcccagaag tagtacgacg cctggaaggc
     3061 actccagtgc tgccccagcc actccattga tctactcgcg cccttttgcc cgacgagaat
     3121 gtccgagccc aacgctcggg ctgctcatcc tgctgcccgc gatactctcg caattatggc
     3181 cagagtgccg tgctgcagca cgcgcggcaa agtccattat atcccagagc gcgcaaatgc
     3241 ctcgccagag cgccttctcg gcaacagccg gcgaattcgc cgaacctggc agccgccatt
     3301 cgccgaacta gaacggtgcc ggggctgttc cggaggcacc acttttcggc ggtcggcacc
     3361 cgaagggcga gccggagtcc actattcccg ccgctcccct tgccccgccc cgcggtcgtc
     3421 atcctgctcc tcctcgtcgt cgaagagaat gcgctcggcg gcgccgtcga ggtcgtcgaa
     3481 ctggccggag cgga
//
LOCUS       DAOTFI010000359         3485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_31935_length_3485_cov_7.446939, whole genome shotgun sequence.
ACCESSION   DAOTFI010000359 DAOTFI010000000
VERSION     DAOTFI010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31935_length_3485_cov_7.446939"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2143)
                     /locus_tag="P5558_23005"
     CDS             complement(<1..2143)
                     /locus_tag="P5558_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRY27253.1"
                     /translation="MVDFASHARERVILFDGGMGTQIQGRELTVEGDFWGQENCSEIL
                     NLSRPDLVREIHLAYLEAGADALETNTFGGSPLTLAEFDLAERAFEINRTAALLARES
                     IERLGADARQRFVVGAIGPGTKLPSLGHVAYREIEAAITLQTAGLIAGGVEALLIETC
                     QDPLQVKAAINGVKAALAAEHRHLPILVQVTIETTGTMLVGTDIAAAITIVDAMGIDG
                     LGLNCATGPKEMAEHLRQLGQTWPGLISVQPNAGLPELKDGRTHYPLGPDELARWLER
                     FVTEDGVNMVGGCCGTTPDHIRAVDAMLRGLAPDGFRPVPLARRPVLQPQLASLFSAQ
                     ALRQENAYLSIGERCNANGSKRFRQLQEAGDWDGCVAMGREQVREGAHTLDLCTAFVG
                     RDEIAEMSEVTKKMRGQVDAPIVFDSTELPVLEAALELYGGKGVLNSINFEDGEKAAE
                     QRMALARKHGAAVIALTIDEAGMAKTADDKLRVCRRLVAFACDRYGLPRSDLLIDPLT
                     FTICTGNEDDRKLALWTLEAIERISAEFPEIQIILGLSNVSFGLNPPARHVLNSVMLD
                     HAMQRGLTGAIVHSSKILPLHKIPQNEVEAAEDLIFNRRREGHDPLQAFMALFADRKA
                     VASEKKQRAAKVEERLAERIVDGDKDGLEADLDEAMTKFPPLTIINEHLLAGMKVVGE
                     LFGAGKMQLPFVLQSAETMKKSVAYLEPFMEK"
     gene            complement(2191..3108)
                     /locus_tag="P5558_23010"
     CDS             complement(2191..3108)
                     /locus_tag="P5558_23010"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRY27254.1"
                     /translation="MTIQTQSSPTSWTLAAELPDVRLSIELFPPKWPAGAMALLEEID
                     VLEALAPAHYTVTSGAGGNGNTETRNVVEAVREHTGRPVVAHMTCIGRSRAEIDAVAE
                     DYWQAGIRHVVALRGDLPKGSEGPYAPRADGYGYASDLVAGLKARHDFEVSVGCYPET
                     HPEAASADADLDSVARKVDAGADWLISQYCFDTDQILRFRDRLAARGIATPFVPGIMP
                     IHNFTQIRRFSERCGAGVPAWLVKLFDGVEPGSDLHKMVAATVASEQCRRLVAEGFGQ
                     LHLYALNRAELSVAVFRLLGGRPALSLAA"
     gene            complement(3108..>3485)
                     /locus_tag="P5558_23015"
     CDS             complement(3108..>3485)
                     /locus_tag="P5558_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY27255.1"
                     /translation="DMYQLPLVDGSVAAVTLHQVLHFADDPLAALAEASRVLRPGGRL
                     VVVDLARHALEWLRADKGHRRLGFAESELADWFAELGLEPAPAVRLAGDGADVCIWLA
                     RKPVAEALHDDTTDDQPAAERAA"
BASE COUNT          494 a         1209 c         1226 g          556 t
ORIGIN      
        1 ccttctccat gaagggctcg agataggcca ccgacttctt catggtctcg gccgactgca
       61 gcacgaaagg gagctgcatt ttgcccgcgc cgaagagctc gcccacgacc ttcatcccgg
      121 cgagcagatg ctcgttgatg atggtgagcg gcgggaactt cgtcatcgcc tcgtcgagat
      181 ccgcctcgag cccgtccttg tcgccgtcga cgatgcgctc ggccagccgc tcctcgacct
      241 tcgccgcccg ctgcttcttc tccgacgcca ccgccttgcg gtcggcgaag agcgccatga
      301 acgcctgcag cgggtcgtgg ccctcccgcc tgcggttgaa gatcagatcc tcggccgcct
      361 ccacctcgtt ctgggggatc ttgtgcaaag gcaggatctt cgacgaatgg acgatggccc
      421 cggtcagccc gcgctgcatc gcgtggtcga gcatcaccga gttgagcaca tgccgggcgg
      481 gcgggttcag gccgaaggag acgttcgaga gcccgaggat gatctggatc tcggggaatt
      541 cggcgctgat ccgctcgatc gcctcgagcg tccagagtgc gagcttcctg tcgtcctcgt
      601 tgccggtgca gatggtgaag gtgagcggat cgatcagcag gtccgagcgc ggcaggccgt
      661 agcggtcgca ggcgaaggcg accagtcggc ggcagacgcg cagcttgtcg tcggccgtct
      721 tggccatgcc ggcctcgtcg atggtcagcg cgatcaccgc cgccccgtgc ttcctggcca
      781 gcgccatgcg ctgctcggcc gccttctcgc catcctcgaa gttgatcgag ttcagcaccc
      841 ccttgccgcc atagagctcg agcgccgcct cgagcaccgg caattcggtc gagtcgaaga
      901 cgatgggcgc gtcgacctgg ccacgcatct tcttcgtcac ctcggacatc tcggcgatct
      961 cgtcgcggcc gacgaaggcg gtgcagagat cgagcgtgtg cgccccttcc cttacctgct
     1021 cgcgccccat ggcgacgcag ccgtcccagt cgccggcctc ctggagctgg cgaaagcgct
     1081 tcgagccatt ggcgttgcag cgctcgccga tcgagagata ggcgttctcc tggcgcagcg
     1141 cctgggccga gaagagcgag gcgagctgcg gctgcagcac gggccggcgg gccaggggca
     1201 ccgggcggaa gccgtccggt gccagcccgc gcagcatggc gtcgacggcg cggatgtggt
     1261 ccggcgtggt gccgcagcag ccgcccacca tgttgacccc gtcctcggtg acgaagcgct
     1321 cgagccacct ggcgagttcg tccggaccca ggggatagtg cgtgcggccg tccttcagct
     1381 cgggcaggcc ggcattgggc tggaccgaga tcagcccggg ccaggtctgg ccgagctggc
     1441 ggagatgctc ggccatctcc ttggggccgg tggcgcagtt cagcccgagc ccatcgatgc
     1501 ccatggcgtc gacgatggtg atggcggcgg cgatgtcggt gccgaccagc atggtgccgg
     1561 tggtctcgat ggtcacctgg acgaggatcg gcagatgccg gtgctcggcg gccagcgccg
     1621 ccttgacccc gttgatcgcc gccttgacct gcagcgggtc ctggcaggtc tcgatcagga
     1681 gcgcttcgac cccgccggcg atgaggccgg ccgtctgcag ggtgatcgcc gcctcgatct
     1741 cgcgataggc gacatggccg aggctcggca gcttggtgcc ggggccgatg gcgccgacga
     1801 cgaagcgctg ccgcgcatcc gcgcccaggc gctcgatgga ctcccgcgcc agaagggccg
     1861 ccgtccggtt gatctcgaag gcccgctcgg cgaggtcgaa ctcggccagc gtcaaggggc
     1921 tgccgccgaa ggtgttcgtt tcgagcgcgt cggcgccggc ctcgaggtag gcgaggtgga
     1981 tctcgcggac gaggtccggg cgcgacaggt tgaggatctc ggagcagttc tcctgccccc
     2041 agaaatcgcc ctcgacggtc agctcgcgac cctggatctg ggtccccatg ccaccgtcga
     2101 agaggatcac ccgctcgcgc gcgtggctgg cgaaatcgac catgatgttc cttctatcgg
     2161 tattttgccg cggcgccttg cgccgccgga tcaggcggcc agcgagagcg ccggccgacc
     2221 gccgagcagg cggaagacgg cgacgctgag ctccgcccgg ttgagcgcgt agaggtggag
     2281 ctggccgaag ccctcggcga ccagccggcg gcactgctcg gaggcgacgg tcgccgccac
     2341 catcttgtgc aggtcgctgc cgggctcgac cccgtcgaag agcttgacca gccaggccgg
     2401 cacgccggcg ccgcagcgct cgctgaagcg ccggatctgg gtgaagttgt ggatcggcat
     2461 gatgccgggc acgaagggtg tggcgatgcc gcgcgccgcc agccggtcgc gaaagcgcag
     2521 gatctggtcg gtgtcgaagc agtactggct gatcagccag tccgcccccg catccacctt
     2581 gcgcgccacg ctgtcgaggt cggcatccgc actcgccgcc tcggggtggg tctccgggta
     2641 gcagccgacg ctgacctcga aatcgtgccg tgccttgagc ccggcgacca gatccgaggc
     2701 gtagccatag ccgtcggccc gcggcgcgta gggcccctcg ctgcccttcg gcagatcgcc
     2761 gcgtagcgcc accacatggc ggatgccggc ctgccagtag tcctcggcca ccgcgtcgat
     2821 ctcggcccgg ctgcggccga tgcaggtcat gtgcgccacg accggccggc cggtatgctc
     2881 gcgcaccgcc tcgaccacgt tgcgcgtctc ggtgttgccg ttgccgccgg cgccgctggt
     2941 gaccgtgtag tgcgccggcg ccagcgcctc gagcacgtcg atctcctcga gcagcgccat
     3001 ggccccggcg ggccatttcg gcgggaagag ctcgatcgac aggcgcacgt ccggcagctc
     3061 cgcggcaagg gtccagctcg tcggcgagct ctgggtctgg atggtcatca ggcggccctc
     3121 tccgcggcgg gttgatcgtc ggtcgtgtcg tcgtgcaagg cttcggctac cggcttcctg
     3181 gcgagccaga tgcagacatc ggcaccatcg ccggcgagcc gcaccgcggg cgccggctcg
     3241 aggccgagct cggcgaacca gtcggcgagc tcgctctcgg cgaagccgag gcgccggtgc
     3301 cccttgtcgg cgcgcagcca ttcgagcgcg tgccgggcga ggtcgacgac aacgagccgc
     3361 ccacccggcc gcagcacgcg tgacgcctcg gccagcgccg ccagggggtc gtccgcgaag
     3421 tgcagcacct ggtgcagggt caccgccgcg acgctgccgt cgaccagcgg cagctggtac
     3481 atgtc
//
LOCUS       DAOTFI010000360         3471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_32098_length_3471_cov_6.899297, whole genome shotgun sequence.
ACCESSION   DAOTFI010000360 DAOTFI010000000
VERSION     DAOTFI010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32098_length_3471_cov_6.899297"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="P5558_23020"
     CDS             <1..538
                     /locus_tag="P5558_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HRY27256.1"
                     /translation="ALVQLYDSTASLQDRTVGTGRLDPALARQYGAGGHVGRASGRGF
                     DSRKCPGYAPYDRLDFTVPVLEGGDVDARVWVRIREVEQSLGLVDQLLATLPEGPVRV
                     DLPACGGEGMALAESFRGDVLAWLRLGPDGRIARCHLRDPSWFQWPLLEAAIEGNIIA
                     DFPICNKSFNCSYSGHDL"
     gene            538..1077
                     /gene="nuoB"
                     /locus_tag="P5558_23025"
     CDS             538..1077
                     /gene="nuoB"
                     /locus_tag="P5558_23025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRY27257.1"
                     /translation="MRKLLFANFFRPPLTEAAPAPGDAALAELGRALERATRRRLGRS
                     LSIREVDAGSCNSCELEIHALNNAFYDVERFGIRFVASPRHADVLMVTGPVTKNMAEA
                     LRRTWDATPDPKWVVAVGDCARDGGLFEASYAVAGGVGAVVPVDLHIKGCPPSPTVLL
                     AGLLALLEQVASGEPAEPG"
     gene            complement(1051..2346)
                     /locus_tag="P5558_23030"
     CDS             complement(1051..2346)
                     /locus_tag="P5558_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27258.1"
                     /translation="MSRLLGITVMPEWIQAEGIDRVLDNLGVAGANAVATSPYVMAPS
                     DDPAAGREPPIDAGAGKVRLLDRDLWGKRELRCVTAPSFVPDRSLYEGLRYQPAAASE
                     LTEREGAKVGAFIEAAQKRGIQVHLQVQAAIPPGYRVQFGGPEAEDEPLLPDGSTHVG
                     RVDKNGSLAAPEVTAYLEALLEDVARAYPTVDAIRLDWPEYPPYTLDSWLFDFSAHAE
                     RAARAKGYDFAAMRDVARRYQAEPARFLEDGLGPLAELGRFKADLAVDLLARARAVLP
                     ANIKLVAHAFPPPWNELSGLDFGRVGAVADDIAVKLYTMHWPMMLRGYLERMGRLDAA
                     GAVAVQKLFDTGEAPLDALRYPEPHEAHPVGAEAQARKIRAAAAAAPGIHAAAHAYGP
                     EGDVLARARVAWEASNRRIWLNRYGYLSDAKLAGLGRLA"
     gene            complement(2389..>3471)
                     /locus_tag="P5558_23035"
     CDS             complement(2389..>3471)
                     /locus_tag="P5558_23035"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY27259.1"
                     /translation="YLADRGHAGKQFMHGMNNNDYMHRPWHLPEDCHVTNWASEQMCR
                     VIKRRDPTRPAFWMLSYTHPHPPLVPLSSYVELYRTLGVDEPLQAEWAKDPAKLPRAL
                     RMIRDFYPAYPPAVIAEIRRAYYALCTHIDHQLRIVLGTLREEGLLDDTIILLAADHG
                     DMLGDFGLFAKRTYFERSANIPMILVGPAGHPRIQAGRTDSRLVGLQDIMPTLLSLAG
                     IAVPQSCDGLDMTAESVRSELYGDCLESAGASRMLHDGRHKLIWYPAGNAFQLFDMED
                     DPSETKDLAGDPAHAATLAGLQERLAERCYGADIDQGWVRDGRLTGFDPGPYTKKMDR
                     SFSGQRGLHYPQPPEAGKEQMVGFPQ"
BASE COUNT          484 a         1218 c         1240 g          529 t
ORIGIN      
        1 ggccctcgtc cagctctacg acagcaccgc ctcgctccag gaccgcacgg tcgggacggg
       61 ccgcctcgac cccgccctcg cccggcaata cggcgccggc ggccatgtcg gccgggcctc
      121 cgggcgcggc ttcgatagcc gcaagtgccc gggctacgcg ccctatgacc ggctcgactt
      181 caccgtgccc gtgctggaag gcggcgatgt cgacgcccgg gtctgggtcc gcatccgcga
      241 ggtcgagcag agcctgggcc tcgtcgacca gctcctcgcc accctccccg aaggccccgt
      301 gcgggtcgac ctgcccgctt gcgggggcga gggcatggcg ctcgccgaga gctttcgcgg
      361 cgacgtgctc gcctggctca ggctcggccc ggacggccgg atcgcccgct gccacctccg
      421 cgacccgtcc tggttccagt ggccactgct cgaggcggcg atcgaaggca acatcatcgc
      481 cgacttcccg atctgcaaca aatcgttcaa ctgctcgtac tcgggacacg acctctgatg
      541 cgtaagttgt tgttcgcgaa cttcttccgc ccgccgctga ccgaagccgc tccggcgcca
      601 ggcgatgcgg ccctggccga gctcggccgg gcgctggagc gggcgacacg gcggcggctc
      661 ggccggagcc tgtcgatccg cgaggtcgat gccggctcct gcaatagctg cgagctcgag
      721 atccacgcgc tgaacaacgc cttctacgat gtcgagcgct tcggcatccg cttcgtcgcc
      781 agcccgcgcc acgccgacgt gctcatggtc accggcccgg tgacgaagaa catggccgaa
      841 gcgcttcgcc gcacctggga tgccaccccc gaccccaaat gggtcgtggc ggtcggcgac
      901 tgcgcgaggg atggcggcct cttcgaggcg agctatgccg tggccggcgg ggtcggcgcc
      961 gtcgtgccgg tcgacctgca catcaagggc tgcccgccct cgccgaccgt cctgctcgca
     1021 ggcctgctcg ccctgctgga gcaggtcgct tcaggcgagc cggccgagcc cggctagctt
     1081 ggcatcgctc agatagccgt agcggttgag ccagatccgc ctgttcgagg cctcccaggc
     1141 gacccgcgca cgggccagca cgtcgccctc cgggccatag gcatgggcgg ccgcgtggat
     1201 gccgggtgct gccgcggcgg cggcccggat cttccgcgcc tgcgcttcgg cgccgaccgg
     1261 gtgggcctcg tgcggctcgg ggtagcggag cgcgtcgagc ggtgcctcgc ccgtgtcgaa
     1321 gagcttctgg accgccaccg cacccgccgc gtccagccgc cccatccgct cgagatagcc
     1381 cctgagcatc atcggccagt gcatggtgta gagcttgacc gcgatgtcgt cggcgaccgc
     1441 cccgacccgg ccgaagtcga gccccgaaag ctcgttccag ggcggcggga aggcatgggc
     1501 caccagcttg atattggccg gcagcacggc ccgcgcacgg gccaggaggt cgaccgcgag
     1561 atccgccttg aagcggccga gctcggccaa aggcccgaga ccgtcctcga gaaagcgcgc
     1621 cggctccgcc tggtagcggc gggccacgtc ccgcatggcg gcgaagtcgt agcccttggc
     1681 cctggccgcg cgttcggcat gggcggagaa gtcgaagagc cagctatcca gcgtgtaggg
     1741 cgggtattcc ggccagtcga ggcgaatggc atcgaccgtc gggtaggccc gggccacgtc
     1801 ctcgagcagg gcttcgaggt aggcggtgac ctcgggtgcc gcgaggctgc cgttcttgtc
     1861 gacccggccg acatgcgtgc tgccgtccgg caacaacggc tcgtcctccg cctcgggccc
     1921 gccgaactgc acccggtagc cgggcggaat ggcggcctgg acctggagat gcacctggat
     1981 gccccgcttc tgcgccgcct cgatgaacgc gccgaccttg gcaccctcgc gctcggtcag
     2041 ctcgctcgcc gcggccggct ggtagcgcaa cccctcgtac agcgagcgat ccggcacgaa
     2101 gctcggtgcc gtcacacagc gcagctcgcg cttgccccag agatcacggt cgagcagccg
     2161 caccttgccg gcgccggcgt cgatcggcgg ctcgcgcccg gcggccgggt catcgctcgg
     2221 cgccatgacg tagggcgagg tggcgaccgc gttggcaccg gccaccccga gattgtcgag
     2281 gacccggtcg atgccctcgg cctggatcca ttcgggcatc accgtgatgc cgagaagccg
     2341 gctcatcgcg ggcccggcga gcttggaaac aagggaaatc ctccctgact actggggaaa
     2401 gccgaccatc tgctccttgc cggcctcggg cggctgcggg tagtggagcc cgcgctggcc
     2461 ggagaagctg cggtccatct tcttcgtgta ggggccgggg tcgaagccgg tgagccggcc
     2521 gtccctgacc cagccctggt cgatgtccgc cccgtagcag cgctcggcga gccgctcctg
     2581 caggccggcc agggtcgcgg catgggcggg gtcgccggcg aggtctttgg tctcgctcgg
     2641 gtcgtcctcc atgtcgaaga gctggaaggc gttgccggcc gggtaccaga tcagcttgtg
     2701 gcggccgtca tgcagcatgc ggctggcgcc ggcgctctcg aggcagtcgc catagagctc
     2761 gctgcgcacg ctctcggccg tcatgtcgag cccgtcgcag ctctgcggca cggcgatccc
     2821 ggcgagcgag agcagggtcg gcatgatgtc ctgcaggccg acgaggcggc tgtcggtgcg
     2881 gcccgcctgg atgcgcggat ggccggccgg gcccacgagg atcatcggga tgttggccga
     2941 gcgctcgaaa taggtgcgct tggcgaagag gccgaaatcg ccgagcatgt cgccgtggtc
     3001 ggccgccagc aggatgatcg tgtcgtcgag cagcccctcc tcgcgcagcg tgccgagcac
     3061 gatgcggagc tggtggtcga tatgcgtgca gagcgcataa taggcgcggc ggatctcggc
     3121 gatcaccgcc ggcggatagg ccgggtagaa gtcgcggatc atccgaaggg cccggggcag
     3181 cttcgccggg tccttcgccc actcggcctg tagcggctcg tcgacgccga gcgtgcggta
     3241 gagctcgaca taggacgaga gcggcaccag cggcgggtgc ggatgggtgt aggacagcat
     3301 ccagaaggcc gggcgggtcg ggtcgcgccg cttgatcacc cggcacatct gctccgacgc
     3361 ccagttggtg acgtggcagt cctccggcag gtgccagggc cggtgcatgt agtcgttgtt
     3421 gttcatgccg tgcatgaact gcttgccggc atggccgcgg tcggcgaggt a
//
LOCUS       DAOTFI010000361         3426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_32683_length_3426_cov_7.583210, whole genome shotgun sequence.
ACCESSION   DAOTFI010000361 DAOTFI010000000
VERSION     DAOTFI010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32683_length_3426_cov_7.583210"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="P5558_23040"
     CDS             complement(<1..1406)
                     /locus_tag="P5558_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRY27260.1"
                     /translation="MPQEARPEQRRKVMRQYQLVELVKSYDPGVDEDLLNRAYVYAMR
                     AHGAQLRASGDPYFHHPVEVAGILAGLKLDAASIATGLLHDTIEDTGATLPEIRRLFG
                     DTIARLVDGVTKLNKIQLQSAQTAQAENFGKLLVAMSEDIRVLLVKLADRLHNMRTLH
                     FIASPERRQRIASETMEIYAPLAERIGMDQLKAELEDLAFAELSPDARESVATRLKQL
                     RRDDTDLVERIVARIKAHLAESGIEADVAGREKSSLSIWRKMQRKNVSFEQLSDIIAF
                     RVLVDSVNECYAALGVIHAGYKMLPGRFKDYISTPKPNGYRSLHTTIIGPEQRKIELQ
                     IRTRQMHEVAELGVAAHWAYKQESRVTEGRQYRWIRELLEILEHAAEPEDFLENTKLA
                     MFQDQVFCFTPRGDLIALPRGATPVDFAYAVHSEVGDHCVGAKVDGRMVQLRHTLENG
                     DQVEIITSRNAWPTAAWER"
     gene            complement(1690..1917)
                     /gene="rpoZ"
                     /locus_tag="P5558_23045"
                     /pseudo
     CDS             complement(1690..1917)
                     /gene="rpoZ"
                     /locus_tag="P5558_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            2206..2853
                     /locus_tag="P5558_23050"
     CDS             2206..2853
                     /locus_tag="P5558_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY27261.1"
                     /translation="MARPAAFKHFHPDERFAVFIDGANLYQTAKALGYDVDYRRLLEV
                     FSGSGRLLRAYYYTALLDEQEYSPIRPLVDWLDYNGYTMVTKPLKEFSQGAGRRKFKG
                     NMDVELTVDVMEVTGAVDHVVLMTGDGDFRRLVEAVQRRGVKVSVISTIKTQPPMIAD
                     ELRRQADFYVELADLTEHIARAHAPRPAVTEDPEEDEYEEEEYEDDDDDVEYIED"
     gene            complement(2920..3237)
                     /locus_tag="P5558_23055"
     CDS             complement(2920..3237)
                     /locus_tag="P5558_23055"
                     /inference="COORDINATES: protein motif:HMM:NF021620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyU family transcriptional regulator"
                     /protein_id="HRY27262.1"
                     /translation="MLGGLIKKLFGGGEATSGESGAGKALGDAVEHKGCKIQPTAMSK
                     GSQFLTAGTISKDFPDGKREHRFVRADTHASADQARDFAIVKGRQIIDEQGDRLFDHA
                     PRG"
BASE COUNT          520 a         1136 c         1166 g          604 t
ORIGIN      
        1 cgctcccagg cggctgtcgg ccaggcgttg cggctggtga tgatctcgac ctggtcgccg
       61 ttctcgaggg tgtggcgaag ctggaccatg cggccgtcga ccttggcccc gacgcaatgg
      121 tcgccgacct cggaatggac ggcataggcg aagtcgaccg gcgtggcgcc gcgcggcagc
      181 gcgatcaggt cgccccgggg cgtgaagcag aacacctggt cttggaacat cgcgagcttg
      241 gtgttctcga ggaagtcctc gggctcggcg gcgtgctcga ggatctccag cagctcgcgg
      301 atccagcgat attgcctgcc ctcggtgacc cggctctcct gcttgtaggc ccagtgcgcg
      361 gcgacgccga gctcggccac ctcgtgcatc tgccgggtgc ggatctgcag ctcgatcttg
      421 cgctgctcgg ggccgatgat ggtggtgtgc agcgagcggt agccgttggg cttgggggtc
      481 gagatgtagt ccttgaagcg cccgggcagc atcttgtagc cggcatggat gacgccgagc
      541 gccgcgtagc actcgttcac gctgtcgacg agcacacgga acgcgatgat gtcggagagc
      601 tgctcgaagc tgacgttctt gcgctgcatc ttgcgccaga tcgacaagga cgacttctcg
      661 cggccggcga catccgcctc gatgccggac tcggcgaggt gcgccttgat gcgggcgacg
      721 atccgctcga ccaggtcggt gtcgtcgcgg cggagctgct tcagccgggt ggcgacgctc
      781 tcgcgcgcat cgggcgacag ctcggcgaat gccaggtcct cgagctctgc cttgagctgg
      841 tccatgccga tccgctcggc gagcggcgcg tagatctcca tcgtctcgct ggcgatgcgc
      901 tggcggcgct cgggcgaggc gatgaagtgc agggtgcgca tgttgtgcag ccggtcggcg
      961 agcttgacca ggagcacgcg gatgtcctcg gacatggcca cgagcagctt gccgaaattc
     1021 tcggcctgcg ccgtctgcgc cgactggagc tggatcttgt tgagcttggt cacgccgtcg
     1081 acgaggcggg cgatggtgtc gccgaagagc cgacggatct cgggcagtgt ggcgccggta
     1141 tcctcgatcg tgtcgtggag cagccccgtg gcgatcgagg cggcatcgag cttgagcccg
     1201 gcgagaatgc cggcgacctc gacggggtga tggaaatagg gatcgcccga cgcccggagc
     1261 tgcgcgccat gcgcgcgcat ggcgtagaca taggcacggt tcagcagatc ctcgtcgacc
     1321 cccgggtcat agctcttgac cagctcgacg agctggtatt gccgcatgac cttgcggcgt
     1381 tgctccggcc tggcttcctg cggcatggtg tcgcaggatt cctcaacggc cagtggcggc
     1441 gccggatcga gcgtcaccgt cgccgtctcg gtcatcgccg tagcagcgcg cgcaacccgc
     1501 ggcaggaccg gcggccgcgg gccgccagcc agcggggggt tgaacgcgac ggtttcatca
     1561 gtcgtcgtcg agcacgtcct gatcgccgta gtccacaccc gccggcatca tgtcgtcctc
     1621 gctgggctgc cggctgggaa tgagcgcctc gagctcctgg gcgagatcgt cggacaggtc
     1681 gccttcatcg tccgaatgcc ggcgcaggcc gtggatcagc tcgtagcgga gatggtcgag
     1741 gtcgatcgtg gaatcggcga tctcgcgcag ggcgacgacg gggttcttgt cgttgtcgcg
     1801 ctccagcgtg agccccgagc ccgccgagat gtcgcgggcg cgctgcgccg cgagcatgac
     1861 cagctcgaac cggctcggca cccgctcgac gcagtcttca accgtaattc gtgccatcga
     1921 tcatccgttc aggggttgtc cattcctcgt ccctacaacc tagtcatgct gcggcgcggc
     1981 gcaagcggca acacccctcg ccgccgagcg gcctttcgtc tccctccgcc gcccgatggc
     2041 ggcctttccc ggcccccaac caggcttgga gcgccggccc gaagccgcta ccctgcctcg
     2101 cgtgttccgc atgggaaccg gcgccctcgg tccggcattc gataaggtcg gcccagattg
     2161 aatgaaccgc ggcaacgacc gcctcacact ccggagtttg gcattatggc caggccagca
     2221 gccttcaagc atttccaccc cgatgagcgc ttcgccgtgt tcatcgacgg tgccaacctc
     2281 taccagaccg ccaaggccct cggctatgac gtggactacc ggcgccttct ggaggtgttc
     2341 tcgggcagcg gacggctgct ccgcgcctat tactacacgg cattgctcga cgagcaggag
     2401 tattcgccca tccggccact ggtcgactgg ctcgattaca atggctacac catggtcacc
     2461 aagccgttga aggagttcag ccagggcgcc gggcgtcgca agttcaaggg caacatggat
     2521 gtcgagctca cggtcgacgt catggaggtc accggcgccg tcgaccacgt cgtgctgatg
     2581 accggcgacg gcgacttccg ccgcctggtc gaggcggtgc agcgccgtgg cgtcaaggtg
     2641 agcgtgatca gcacgatcaa gacgcagccg ccgatgatcg ccgacgagct ccgccgtcag
     2701 gccgatttct acgtcgagct cgccgatctc accgagcaca tcgcccgggc ccatgcaccg
     2761 cgaccggcgg tgacagagga ccccgaggag gatgagtacg aggaagagga gtacgaggac
     2821 gacgacgacg acgtcgagta catcgaggac tgacgatcaa cgcgtcgccc ccgcgcatcg
     2881 cgctgcccgg ctgcagccgg gcagcgcgag ctcgggctgc tagccccgag gcgcatggtc
     2941 gaagagccgg tcgccctgct cgtcgatgat ctgccgtccc ttgacgatgg cgaagtcacg
     3001 ggcctggtcg gccgaggcat gggtgtcggc gcggacgaag cgatgctcgc gcttgccgtc
     3061 cggaaagtcc ttgctgatgg tcccggcggt gaggaactgg ctgcccttgc tcatggccgt
     3121 cggctggatc ttgcagccct tgtgctcgac cgcatcgccg agcgccttgc cggcgccgga
     3181 ctcgccactc gtcgcctcgc ctcctccgaa gagcttcttg atcaacccgc cgagcatcga
     3241 cttactccct gttcgcgcat ggcaccctgg cccgtcgccg gtggccgggc tcaataggtc
     3301 aggagcggcg gcagcgtccc ggcttccgcg atccagcgtg ccaccatgct ctcggccggg
     3361 acggcgcgcg tcaacttctt ccgggcgttg acctcctcca tccgcgtcgc gaggttggcg
     3421 gcgttg
//
LOCUS       DAOTFI010000362         3365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_33443_length_3365_cov_7.655891, whole genome shotgun sequence.
ACCESSION   DAOTFI010000362 DAOTFI010000000
VERSION     DAOTFI010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33443_length_3365_cov_7.655891"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(743..2581)
                     /gene="cobT"
                     /locus_tag="P5558_23060"
     CDS             complement(743..2581)
                     /gene="cobT"
                     /locus_tag="P5558_23060"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HRY27263.1"
                     /translation="MKERLDGFEQALGAAARAIAEEPKLEVVLRSGNRRGPAGAGEVI
                     RINPPRQDLPTGEIARVRGEADSQALRRRFHDGELHRRMVPEGELATMLFDGLEQLRV
                     ELVGGRDMAGVRANLEAVFRQRGAPATAGEAVPDSNVAEVVELYMRERLQGYRLNAAQ
                     SRQLDAWRDWLDGKVEAVLPELDARAESQDAFARRVRELMTDLGLSEALVEEPEPDSR
                     DDESEEESGDQGPSEPSPDQDDDGGDEPQRAEAQDSAEDGTPEDLMAEEAAASDDELQ
                     SGAEEEAGEDPRTNRPKEHPPGANQLAYRVFTSQFDEVVGVAELCGAAELTRLRAQLD
                     ASLSRFEGMIGRIANRLQRKLMAQQQRSWDFDLDEGILDAARLARIVVNPTLGLSYKM
                     EKETEFRDTVVTLLIDNSGSMRGRPIAVAAMTADILARTLERCGVKVEILGFTTRAWK
                     GGQAREAWLRAGKPAAPGRLNDLRHIVYKAADAPWRRARPNLGLMLREGLLKENIDGE
                     AILWAHERLLGRVEQRRILMVISDGAPVDDSTLSANPGNYLEKHLREVIDWIETRSEV
                     ELLAIGIGHDVTRYYRRAVTISDPEQLGGTVLGQFSALFDPKRRAA"
     gene            complement(2613..>3365)
                     /locus_tag="P5558_23065"
     CDS             complement(2613..>3365)
                     /locus_tag="P5558_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY27264.1"
                     /translation="THIEQVAARLNWPCVRVNLDSHISRIDLIGKDAIVLKEGQQVTE
                     YREGILPWSLQHPVALVFDEYDAGRPDVMFVIQRVLEAQGRMTLLDQNKILRPHPSFR
                     LFATANTVGLGDTSGLYHGTQQINQGQMDRWSIVVQLNYLPHDDECRIVLAKCPRYDT
                     AEGRKTIASMVRLADLTRAGFVNGDISTVMSPRTVITWAENTEIFGDVGFAFRVTFIN
                     KCDEAERAIIAEYYQRCFGKELPESAANLLVA"
BASE COUNT          475 a         1195 c         1151 g          544 t
ORIGIN      
        1 cacgagacca ttttaacctt tcgacctaat agcgcagctt tccgtgtcag cttaatcgcc
       61 gccgtccgcg catcacgcac ccagaacaac cggtggggcc agcggggaag cgccgcctcc
      121 ccgcgctact tcgccttctc ttccccccga tccggaggtc gccgatgacg gccgcctgca
      181 tgaagcgctc cccttccggt acggggggag atcggatcgg aacggcggtc gggacgacgc
      241 cgcagcccga cgccatcacg aacgcttatg aggccctgcc gccatcgccc gccggcccgc
      301 cccttcggcc cggattcgcc gtcgcggctc ccgggttggc gcgtcccgcg cgacattctc
      361 gcccgggttg gcgcatcggc tgcgacaagg cccgccggaa tgtcgcggat tccgcgacat
      421 aacgattcgt gatggcgcat ccgccgcgac attcacccgc aaatgtagcg gattctgcaa
      481 cagccgaccg cgaacgaacc cgccgaatcg acgagcgaac ccgggattca ggcgagcgaa
      541 cccggggatt cgacgaacga acccgaccgg cgaccaacga acccgggggc catcaacctc
      601 ttgtcggaga acgattttta gccgtttcgg gctgccggcc agcggtccct ggggtctcgg
      661 aacccgatat tgccgaagaa acccggcaga aggcttgcgc cgcccgtcga aataggttat
      721 aaatcctaaa cgcccaaaag aatcaggcgg cgcggcgctt cgggtcgaag agggccgaga
      781 actggccgag cacggtgccg ccgagctgct cggggtcgga gatggtgacg gcgcggcggt
      841 agtagcgggt cacgtcgtgg ccgatgccga tggcgagcag ctccacctcg gagcgggtct
      901 cgatccagtc gatgacctcg cgcagatgct tctcgaggta gttgccgggg ttggccgaga
      961 gcgtgctgtc gtcgaccggg gcaccgtccg agatgaccat caggatgcgc cgctgctcga
     1021 cccggccgag caggcgctcg tgcgcccaga gtatcgcctc gccgtcgatg ttctccttga
     1081 gcagcccttc gcgcagcatg agaccgagat tgggccgggc ccgccgccag ggcgcgtcgg
     1141 cggccttgta gacgatgtgg cgaaggtcgt tgagccggcc gggtgcggca ggcttgccgg
     1201 cccgcagcca ggcctcgcgc gcctgcccgc ccttccaggc gcgggtggtg aagcccagga
     1261 tctccacctt gacgccgcag cgctcgaggg tgcgggcgag gatgtccgcg gtcatggcgg
     1321 cgaccgcgat cggccggccg cgcatcgagc ccgagttgtc gatcaggagg gtcaccaccg
     1381 tgtcccggaa ctcggtctcc ttctccatct tgtaggagag gccgagggtc gggttgacga
     1441 cgatccgggc gaggcgtgcg gcatccagga tgccctcgtc gaggtcgaag tcccaggagc
     1501 gctgctgctg cgccatgagc ttgcgctgca gccggttggc gatccgcccg atcatcccct
     1561 cgaagcgcga gaggctggcg tcgagctggg cccgcaggcg cgtcagctcg gccgccccgc
     1621 agagctcggc gaccccgacc acctcgtcga actgcgacgt gaagacgcga taggcgagct
     1681 ggttcgcccc gggcgggtgc tccttcggcc ggttggtgcg cgggtcctcg cccgcctcct
     1741 cctccgcacc ggactgcagc tcgtcgtcgc tcgccgcggc ctcctcggcc atcaggtcct
     1801 cgggcgtgcc gtcctcggcg ctgtcctgcg cctcggcccg ctgcggctcg tcgccgccgt
     1861 cgtcgtcctg gtccggcgag ggttcgctcg gcccctggtc gccgctctcc tcctcgctct
     1921 cgtcgtcccg gctgtcgggc tcgggctcct cgaccagcgc ctcgctgagg cccagatcgg
     1981 tcatcagctc gcgcacccgc cgggcgaagg cgtcctggct ctcggcccgg gcgtcgagct
     2041 cgggcagcac cgcctccacc ttgccgtcga gccagtcgcg ccaggcgtcg agctggcggc
     2101 tctgggcggc gttgagccgg tagccctgca gccgctcgcg catgtagagc tcgaccacct
     2161 cggcgacgtt ggaatcgggg accgcctcgc cggccgtggc gggcgcgccg cgctggcgga
     2221 acacggcctc gagattggcc ctgaccccgg ccatgtcgcg gccgccgacc agctcgaccc
     2281 ggagctgctc gagcccgtcg aacagcatgg tggcgagctc gccctcgggc accatgcggc
     2341 gatgcagctc gccgtcgtgg aagcgccgcc gcagcgcctg gctgtcggcc tcgccccgga
     2401 cccgggcgat ctcgccggtc ggcaggtcct ggcgcggcgg gttgatgcgg atcacctcgc
     2461 cggccccggc gggcccccgc cggttgcccg agcgcagcac cacctcgagc ttcggctcct
     2521 cggcgatcgc ccgcgccgcg gcccccaggg cctgctcgaa tccgtcgagg cgctccttca
     2581 acgccgctcc atcccgctgc tgcccctgcc gctcaggcga ccaggagatt ggccgccgat
     2641 tcgggcagct ccttgccgaa gcaccgctgg tagtactcgg cgatgatcgc gcgctcggcc
     2701 tcgtcgcact tgttgatgaa ggtcacccgg aacgcgaagc cgacatcgcc gaagatctcg
     2761 gtgttctcgg cccaggtgat caccgtgcgc ggcgacatca ccgtcgagat gtcgccgttg
     2821 acgaagcccg cgcgggtcag gtcggcgagg cggaccatgc tggcgatggt cttgcgcccc
     2881 tcggccgtgt cgtagcgcgg gcacttggcg aggacgatgc ggcattcgtc gtcgtgcggc
     2941 aggtagttga gctgcacgac gatgctccag cgatccatct ggccctggtt gatctgctgg
     3001 gtgccgtgat agaggcccga ggtgtcgccg agcccgaccg tgttggcggt ggcgaacagg
     3061 cgaaagctcg gatgcggcct caggatcttg ttctggtcga gcagggtcat gcggccctgc
     3121 gcctcgagca cccgctggat cacgaacatg acgtcgggcc ggccggcatc gtactcgtcg
     3181 aacaccagcg ccaccggatg ctgcaaggac cagggcagga tgccctcgcg gtactcggtg
     3241 acctgctggc cctccttcag gacaatcgcg tccttgccga tcaggtcgat gcggctgatg
     3301 tggctgtcga ggttgacgcg cacgcacggc cagttcagcc gggcggcgac ctgctcgata
     3361 tgcgt
//
LOCUS       DAOTFI010000363         3357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_33541_length_3357_cov_5.420351, whole genome shotgun sequence.
ACCESSION   DAOTFI010000363 DAOTFI010000000
VERSION     DAOTFI010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33541_length_3357_cov_5.420351"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..426
                     /locus_tag="P5558_23070"
     CDS             214..426
                     /locus_tag="P5558_23070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY27265.1"
                     /translation="MATLASPQSQPAAEPETVSVDDAARWAGLGRSTLYAAIGSGALP
                     TIKLGKRRLVRLATLRAWLAALEGRS"
     gene            423..1295
                     /locus_tag="P5558_23075"
     CDS             423..1295
                     /locus_tag="P5558_23075"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRY27266.1"
                     /translation="MRSALVTVDVGAPAARPGTRRRPCPACDRGPRDTALAVTRKPDG
                     GLVWFCHRCGVRGAANDDRPGAPPAAVVRRTAPPAVDGNAATLRDRLWRASRPIDTDS
                     LAAAYLLHRGCALPPVGADLRWLPVLHHPSDWAGPAMLALVTDALTGSPLTLHRTWLA
                     PDGRGKAPVEPPRLLLRGLAKAGGVVRLWPDEAVTGGLGIAEGIETALAAARGFEPVW
                     ACLDAGNLASLPILPGVEALTVFADHDPAGLAAADRVCAAWRAAGAEARRWLDQRPGA
                     DCNDFVNEMCHDPR"
     gene            1282..2832
                     /locus_tag="P5558_23080"
     CDS             1282..2832
                     /locus_tag="P5558_23080"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HRY27267.1"
                     /translation="MTRDDEFRRSIDRLPAENPPALPANDNTDWPEPDRSVLELRQLT
                     PPALPLHVLGSRWAGFVRGAAAAAGAPPDYVALALLAAAGALLANGRRIEAWDGWVEP
                     PLLFGAVIGRPSTNKSPAIGIIRDLMAKLEAEQNADHAERRRRHRTDTEAATAHMEAW
                     RRDVQDAVKHHLPPPCQPAEAEPPPAPRRRRLLVNDATTEQLCRLLADNTHGLLQCRD
                     ELSGWFGGMNRYSTGQSDRAFWLEAYGGRPFVIDRVRDDEPVMVPALSLAVLGGMQPD
                     RLAEILAGADDGLTARILFVWPEPVPPTRPEVPLDAKAAFRALRQLAGLERLERPEGD
                     RGPGWKPISLSEPALQALQDFRRRRAADERQAGGQLLSWLGKQNGTLLRLALILAYLR
                     WAMDEPAAPAPLEIGKALLDDAIELIEAYFIPMARRAFGEASLPEIERDAHALARWIR
                     ARLPGLQTVNARDLRRARAIPTDQAPRYDAAIGELVEAGWLRQPPSSGSGRPRKDYLI
                     NPKITAPS"
BASE COUNT          518 a         1249 c         1120 g          470 t
ORIGIN      
        1 tcgagcagat cccgacggac ctgcgcaatc cgggtcgaca ggtgcgactg cgccccgacg
       61 cgataggaaa attgacccaa aagatcctct atgaacgcgc cgaagccgaa tgtcaaagga
      121 agcagtccga aaaagaatcc gaggctactt ctttaggaat ttgatctcct atcgcgcatc
      181 ttgccggcca tccacaaacg acggagttca gagatggcca cacttgcttc accccagtca
      241 cagccggccg ccgagcccga gaccgtctcg gtcgacgacg cggcccggtg ggccgggctc
      301 ggccggtcga cgctctatgc ggcgatcggc agcggtgccc tgccgacgat caagctcggc
      361 aagcggcggc tggtccggct cgccaccctg cgcgcctggc tggcggcgct ggagggccgc
      421 tcgtgaggtc ggccctcgtc accgtcgacg tcggggcacc ggcggcgcgt ccgggaacgc
      481 ggcgccggcc ttgccccgcc tgcgatcgcg ggccgcgcga cacggcgctc gccgtcaccc
      541 gcaagcccga tggcgggctg gtctggttct gtcaccgctg cggcgtgcgg ggtgccgcga
      601 atgacgacag gccgggggca ccgccagcgg ccgttgtcag gcgcaccgcc ccgccggcgg
      661 tcgacggcaa tgccgcaacg ctgcgcgacc ggctctggcg ggcgagccgg ccgatcgaca
      721 cggactcgct cgccgccgcc tacctcctgc atcgcggctg cgccctaccg cccgtcgggg
      781 ccgatctgcg ctggctgccg gtgctgcacc atccctcgga ctgggccggc ccggcaatgc
      841 tggccctcgt caccgatgcg ctgaccggaa gtccgctcac cctgcaccgt acctggctcg
      901 ccccggacgg acgcggcaag gcgccggtcg agccgccgcg gctgctgctc aggggcctcg
      961 ccaaggccgg cggtgtggtc cggctctggc cggacgaggc cgtgaccggc ggcctcggga
     1021 tcgccgaggg gatcgagacc gcgctcgccg ccgcccgcgg cttcgagccg gtctgggcct
     1081 gcctcgacgc cggcaacctg gcgagcctgc caatcctgcc gggcgtcgag gccctcaccg
     1141 tcttcgccga ccacgatccg gccggcctcg ccgccgccga ccgcgtctgc gccgcctggc
     1201 gcgccgccgg ggccgaggcc cgccgctggc tcgaccagcg ccccggcgcc gactgcaacg
     1261 acttcgtcaa cgagatgtgc catgacccgc gatgacgagt tccgtcggag catcgaccgc
     1321 ctgcctgccg agaacccgcc ggcgctgccg gccaacgaca acacggactg gcccgagccg
     1381 gaccgctcgg tgctggagtt gcgccagctt acaccgcccg ccctgccgct gcacgtcctg
     1441 gggtctcgct gggcggggtt cgtccgcgga gccgccgcgg ccgcaggggc gccaccggac
     1501 tatgtcgccc tggcgcttct cgccgcagcc ggcgcgctgc tcgccaacgg ccgccggata
     1561 gaggcttggg acggctgggt cgagccgcca ctgctgttcg gtgcggtgat tggccggccg
     1621 tcgaccaaca agagcccggc gatcggtatc atccgcgacc tcatggccaa gctcgaggcc
     1681 gagcagaatg ccgatcatgc cgaacggcgc cgacgccacc gcacggacac agaggcagcc
     1741 acggcccaca tggaagcctg gcgtcgcgac gtgcaggacg ccgtgaagca tcacctcccg
     1801 ccaccctgcc agccggccga ggccgagccc ccgccggcgc cgcggcggcg aaggctgctg
     1861 gtcaatgacg cgaccaccga acagctctgc cggctgcttg ccgacaacac gcacggcctg
     1921 ctgcaatgtc gcgacgagct gagcggctgg ttcggcggca tgaaccgcta cagcactggc
     1981 cagtccgacc gggccttctg gctcgaggcc tatggtggcc gccccttcgt catcgaccgg
     2041 gtccgcgatg acgagcctgt catggtcccg gcactcagcc tggccgtgct cggcggcatg
     2101 cagccggacc ggctggccga gatcctcgcc ggggccgatg acgggctcac cgcacgcata
     2161 ctctttgtct ggcccgagcc ggtcccgccg acgaggccgg aggtgcccct cgatgccaag
     2221 gcggcattcc gcgccctgcg gcagctggcc gggctcgagc ggctcgagcg gcccgagggc
     2281 gacagggggc caggctggaa gccgatcagc ctgtccgagc ccgccctcca ggcgctgcag
     2341 gacttccgcc ggcgccgggc ggcggacgag cggcaggcgg gcggccagct gctctcctgg
     2401 ctcggcaaac agaacggcac gctgctgcga ctggccctga tccttgccta tctgcgctgg
     2461 gcgatggacg agccggcggc acccgcgcct ctggagatcg gcaaggcact cctcgacgac
     2521 gcgatcgagc tgatcgaggc ctacttcatc cccatggcgc gacgcgcctt cggcgaggcg
     2581 tcgctgccgg agatcgagcg cgacgcgcat gctctcgctc gctggatcag agcccggctg
     2641 ccaggccttc aaacggtcaa tgcccgcgac ctgaggcgtg ctcgggcaat cccgaccgac
     2701 caggcgcccc gctacgatgc cgccatcggc gagctggtcg aggccggctg gctcaggcag
     2761 ccgccatcct ccggcagcgg ccgcccgcgc aaggactacc tgatcaaccc gaaaatcacc
     2821 gccccaagct gatcgccatt ccggccccga taaccccatt cgaggccatg ccaaaactgc
     2881 caaaacctgc caaaaccccc ttttggcagg ttttggcact tttggcacaa ggcccagtcc
     2941 gaaactcccg gatcacacct caagcactca cggtcgacgc gttccgcgcc gaattatccc
     3001 cgcaaccctg aaaacaggtg atatcaggcg gttctcccaa aaatcggcct cctgagaaca
     3061 agtaatttca ggcgaagcga cgacacaccg gcagagccac gctctcttgc gctcttggta
     3121 aggcgtttgc agttgcgcca gatggcgtcc gacgggcgcc ggttcggcta gcgaccggct
     3181 actgatcctg ctccgccgcg agggccgcga ggtgagccac aaacgcctgt ccggccctat
     3241 cgggtggagc ggctgacggt acgcaagcgc ggcgtcagca aaagcgctct cggcacgcgg
     3301 acggtaagcg tccgctgacc tacgcggcct tttgcgttga cgcggtggcg gctcgtg
//
LOCUS       DAOTFI010000364         3305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_34180_length_3305_cov_5.746462, whole genome shotgun sequence.
ACCESSION   DAOTFI010000364 DAOTFI010000000
VERSION     DAOTFI010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34180_length_3305_cov_5.746462"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="P5558_23085"
     CDS             complement(<1..488)
                     /locus_tag="P5558_23085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY27268.1"
                     /translation="MSVAVFGSVVADTRLVTEAPARLHTSNPATSRAAAGGVARNVAI
                     ALARLGLPVRLSSRVGEDAEGRALLEGLRAAGVDTDGIGRSATQPTARYWAVLEPSGE
                     LVIGLADMAVLEEHGPAEVAPVAARRADAWFLDCNLPATTLALLLDHPARPALVAADT
                     VSA"
     gene            complement(532..1407)
                     /locus_tag="P5558_23090"
     CDS             complement(532..1407)
                     /locus_tag="P5558_23090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRY27269.1"
                     /translation="MEWRGRRGSKNVIDVRGRGRGGGRSMGRIGGFGGIGAVAIVLIG
                     LFLGVDLTPLLGLGSGGGMQSAPPVQQAGPNRIDDEAEEFVSVVLADTEEVWSREFEA
                     RGARYEVPELVLFTGAVDSACGTASSAVGPFYCPGDHRVYLDTAFFQTLESQLGARGD
                     FARAYVIAHEVGHHVQNLIGVMDQVNEARRSVSEVEANALSVRVELQADCLAGMWARG
                     ADEMFGTLERGDIDEALDAAARIGDDTLQRQSQGRVVPDSFTHGTSEQRQAWFYQGFE
                     QGSLESCDTFSIDRV"
     gene            complement(1493..2188)
                     /locus_tag="P5558_23095"
     CDS             complement(1493..2188)
                     /locus_tag="P5558_23095"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY27270.1"
                     /translation="MAIAAAAGLMRGFAGVGSGMLMAPFFVHLFGPVATVGVITLLEG
                     VATAQLLPSLHRQVAWRTIAPMGLAAAFAMPLGSWLLVTLDPASLQLGISVLVTISAL
                     LLLSGWRYMGPRPAVATAGVGMLSGVLMAVSSVGNPPVMLYLLSGGDTAATNRANFTG
                     YFALTLMALTIMMLIGGLVPRFVVALALVLLPGYLVATWLGSRLFQRSSDLTYRRVAL
                     GILLIAGLYGMTL"
     gene            complement(2277..2519)
                     /gene="purS"
                     /locus_tag="P5558_23100"
     CDS             complement(2277..2519)
                     /gene="purS"
                     /locus_tag="P5558_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRY27271.1"
                     /translation="MQARIAIRFRPGVLDPEATAIQRSLHGLGFTEVEAVAKVKVIEL
                     TLAATDRPTAEARLRQMCEKLLANPVIETFEIELGG"
     gene            complement(2535..3299)
                     /locus_tag="P5558_23105"
     CDS             complement(2535..3299)
                     /locus_tag="P5558_23105"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRY27272.1"
                     /translation="MARRKQVYEGKAKILFEGPESGTLIQYFKDDATAFNNKKHAVIT
                     GKGVLNNRISEYLMMKLHDVGIPTHFVRRLNMREQLIREVEIIPIEVVVRNIAAGSLG
                     KRLGIEDGTLLPRSIVEYYLKSDELGDPMVSEEHITAFGWASYQEIDEIMSMSLRIND
                     FLSGLFLGVGLKLVDFKVEFGRLWEDEDMRLVLADEISPDNCRLWDVKTNERLDKDRF
                     RQDLGKVEEGYQEIARRLGILPEAGPRDMRGPETMQ"
BASE COUNT          505 a         1095 c         1238 g          467 t
ORIGIN      
        1 gcggagacgg tatcggcggc gacgagggcc ggccgtgccg gatggtcgag gagcaaagcg
       61 agcgttgtgg ccggcaggtt gcagtcgagg aaccaggcgt ccgcgcgccg ggccgccacc
      121 ggcgccactt ccgccgggcc gtgctcctcg aggacggcca tgtcggcgag gccgatcacc
      181 agctcgcccg agggctcgag caccgcccag tagcgggcgg tgggctgggt ggcggagcgg
      241 ccgatgccgt cggtgtcgac cccggcggcc cgcagacctt ccaacagcgc gcggccttcc
      301 gcatcctcgc cgacgcggct cgagagccgg accggcaggc cgagccgcgc cagggcgatc
      361 gcgacattgc gggcgacgcc gccggcggca gcgcggctgg tggcggggtt ggaggtgtgg
      421 agccgggccg gtgcctcggt cacgagccgc gtgtcggcga ccaccgagcc gaagaccgcc
      481 acactcatgc ctggcctgcc catgcgcccg ctgccgatcg ggcccggccg gtcagacccg
      541 gtcgatgctg aaggtgtcgc aggactccag cgagccctgc tcgaagccct ggtagaacca
      601 ggcctgacgc tgctcggagg tgccgtgggt gaagctgtcg gggacgacgc ggccctggct
      661 ctggcgctgc agcgtgtcgt cgccgatgcg ggccgcggca tcgagcgcct cgtcgatgtc
      721 gccgcgctcg agcgtgccga acatctcgtc ggcgcccctc gcccacatgc cggcgaggca
      781 gtccgcctgc agctcgaccc tgaccgagag ggcattcgcc tcgacctcgg agacgctccg
      841 gcgcgcctcg ttgacctggt ccatgacacc gatcaggttc tgcacgtggt ggccgacctc
      901 gtgcgcgatg acataggcgc gggcgaagtc gcccctggcg ccgagctggc tctcgagcgt
      961 ctggaagaag gccgtgtcca gatagacccg gtggtcgccc gggcagtaga acgggccgac
     1021 ggcggagctt gccgtgccgc aggccgagtc gacggcgccg gtgaagagga cgagctccgg
     1081 cacctcgtag cgggcaccgc gcgcctcgaa ctcccgcgac cagacctcct cggtgtcggc
     1141 gagcaccacc gagacgaact cctcggcctc gtcgtcgatc cggttgggcc cggcctgctg
     1201 gaccggcggg gccgactgca tgccgccgcc cgagccgagg ccgaggagcg gcgtcaggtc
     1261 gacgccgagg aagaggccga tgaggacgat ggcgacggca ccgatgccgc cgaagccgcc
     1321 gatccggccc atcgagcgac cgccgccgcg gccacgcccg cgcacgtcga tgacgttctt
     1381 gctgccacgc cggccacgcc actccatctt ccgttcccct gcccgatcct ggcgcctcgg
     1441 gcggagcgtg cgggatgcgc cccgcccggt caacggcgaa gattcgcccg atctacaggg
     1501 tcatgccgta gagcccggcg atgagcagga tgccgagcgc cacccgccgg taggtgagat
     1561 cggacgagcg ctggaagagc cggctgccga gccaggtggc gacgaggtag ccgggcagga
     1621 gcacgagggc cagcgccacg acgaagcgcg ggacgagccc gccgatcagc atcatgatgg
     1681 tgagcgccat gagcgtcagc gcgaagtagc ccgtgaagtt ggcgcggttg gtcgccgccg
     1741 tgtcgccgcc ggagaggagg tagagcatga ccggcgggtt gccgacgctg gagaccgcca
     1801 tcagcacgcc ggagagcatg ccgaccccgg cggtcgccac ggccggcctg ggccccatgt
     1861 agcgccagcc gctgagcagg aggagggccg agatggtgac caggacggag atgccgagct
     1921 gcaggctcgc cgggtccagc gtcaccagca gccagctgcc gagcggcatg gcgaaggcgg
     1981 cggcgaggcc catgggggcg atggtccgcc aggcgacctg ccggtgcagg gacggcagga
     2041 gctgggcggt ggcgacgcct tcgagcagcg tgatgacacc gaccgtcgcc accgggccga
     2101 agagatggac gaagaagggc gccatcagca tgcccgagcc gaccccggcg aagccgcgca
     2161 tcagcccggc cgccgcggcg atggccatgc cggcagcgat gtcgggggcg agggcggcga
     2221 aaaagttcgg caggatcggg cggactcccc ggatggccct cgccgggcca gtaggctcag
     2281 ccgccgagct cgatctcgaa ggtctcgatc accgggttgg cgaggagctt ctcgcacatc
     2341 tggcggaggc gggcctcggc ggtggggcgg tcggttgcgg cgagggtgag ctcgatgacc
     2401 ttgaccttgg ccacggcctc gacctcggtg aagccgaggc cgtgcagcga gcgctggatg
     2461 gcggtggcct cggggtcgag gacgcccggg cggaagcgga tggcgatgcg ggcctgcatg
     2521 gtcgaaatgg tcgcctattg catcgtttct gggccgcgca tgtcgcgcgg gccggcctcg
     2581 ggcaggatgc cgaggcggcg ggcgatttcc tggtagccct cctcgacctt gccgaggtcc
     2641 tggcggaagc ggtccttgtc gagccgctcg ttggtcttga cgtcccagag ccggcaattg
     2701 tccgggctga tctcgtcggc gagcacgagg cgcatgtcct cgtcctccca caggcggccg
     2761 aactcgacct tgaagtcgac gagcttgagg ccgacgccga ggaagaggcc ggagaggaag
     2821 tcgttgatcc tgagcgacat gctcatgatc tcgtcgatct cctggtagga ggcccagccg
     2881 aaggcggtga tgtgctcctc ggagaccatg gggtcgccca gctcgtccga cttgaggtag
     2941 tactcgacga tcgagcgcgg caggagggtg ccgtcctcga tgccgaggcg cttgccgagc
     3001 gagcccgcgg cgatgttgcg caccaccacc tcgatcggga tgatctcgac ctcgcggatc
     3061 aactgctcgc gcatgttgag gcggcggacg aagtgggtcg ggatgccgac gtcgtgcagc
     3121 ttcatcatca ggtattcgga gatgcggttg ttcagcaccc ccttgccggt gatcaccgcg
     3181 tgcttcttgt tgttgaacgc ggtggcgtcg tccttgaagt actggatgag cgtgccggat
     3241 tccgggccct cgaagagaat cttggccttg ccctcgtaga cctgcttgcg ccgggccatg
     3301 caatc
//
LOCUS       DAOTFI010000365         3299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_34249_length_3299_cov_6.848952, whole genome shotgun sequence.
ACCESSION   DAOTFI010000365 DAOTFI010000000
VERSION     DAOTFI010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34249_length_3299_cov_6.848952"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1065
                     /locus_tag="P5558_23110"
     CDS             259..1065
                     /locus_tag="P5558_23110"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRY27273.1"
                     /translation="MTSTSFTLAASSEMIFRDLPVAERIRRIHDAGFAVEIWDWRNHD
                     IDALESLRGEGVVFTSMTGYTTGTLGDPEGAEELLRTAAESIPVGKRIGAWALNLHGT
                     GLDDKGLPVKALAAGEIASEEMWATARHTLQRIAELGEREGVTFVLENLNLAVDHPGT
                     PFARAEDTLALVSGVDSPHLKLNLDLYHAQIGEGNLIELCRAALPWIGEIQVADVPGR
                     CEPGTGEINYGGVARALREMGYAGVVGLEAFASSDSDLALRRFREAFSLA"
     gene            1119..1421
                     /locus_tag="P5558_23115"
     CDS             1119..1421
                     /locus_tag="P5558_23115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRY27274.1"
                     /translation="MGATLVLMSQNAAPAETLSQRELRNESGRVLRSVAEGRSYVLTN
                     GGVPVGRIVPIDAPSPGLTIARAARRRGGWAALGIRRKHSDRSLAELLDDLREDRV"
     gene            1418..1831
                     /locus_tag="P5558_23120"
     CDS             1418..1831
                     /locus_tag="P5558_23120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY27275.1"
                     /translation="MSPPPVTYVDTSALGALLIEQPESEALAEWLDNTTDHLVSCDLL
                     ETELRRLAVREGLDQHDVTAILDGVALAALDRAAYRGAGILPMPYLRALDALHLEAAL
                     RLAAHSVLTYNHRLAAAARTLGLTVVNPGRPDEPR"
     gene            complement(1835..3001)
                     /locus_tag="P5558_23125"
     CDS             complement(1835..3001)
                     /locus_tag="P5558_23125"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY27276.1"
                     /translation="MTVLPEVEPLRIGILGAARIAPLSIVSPAQATGTRLVAVAARDR
                     SRAADFAAANGVERVVESYDALLADPEIEAVYNPLANGLHAPWNAKIAAAGKHVLAEK
                     PSAANAAEAREVADAVRASGVTPSRQCADPGTRGTSESGEARRREGQIVLMEAFHYPY
                     HPLFQRAVALIEEGAIGEVRHIDVPLLMPDPGADDPRWRLDLAGGSTMDLGCYSLSCL
                     ALLGERFCGGSLTIREADAVERDGHPGVDSHLFVSGDFPSGATGSGGSDMDADDWSFT
                     LVVTGSEGELVIPSFPLPHQDDRLILRHTAAAPRARRRPDEAADLTSEREDDVVEHLG
                     NRSSYTYQLEAFAAAVRGGAPVVTDAEFAVRNMALVDASYTAAGLPVRESAARS"
     gene            complement(2998..>3299)
                     /locus_tag="P5558_23130"
     CDS             complement(2998..>3299)
                     /locus_tag="P5558_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27277.1"
                     /translation="AAKINGVLDDRFTRVAEGEKGYLPLGGGTTLSGWPSNSSWDFVA
                     IGKGHDVAFWTEFLRTLQEIDPDLPCNIEHEDAELGQLEGLEFAARNMLAAEQAL"
BASE COUNT          531 a         1121 c         1137 g          510 t
ORIGIN      
        1 ctggctcgac ccgagccacg gcggcctgaa cctcggtttc ccgcagaaca cctgagcatc
       61 cagaaccgca ctgtctggag gccgcggtgc gaactcgggc caggttttta ttgtggggcc
      121 tggctataag caggccccag attgagaacc tggccccagt tctcgaaaac ccggccccac
      181 aacgagaacc cggcaccagt gctcggaagg ggcgggtcaa gtggcccgat tcgacgagat
      241 ctctgagggt tgacagccat gacgtccacg tctttcaccc tggcggcgag cagcgagatg
      301 atctttcgcg atctgcctgt ggcagagcgc attcgacgca tccacgacgc cggcttcgcc
      361 gtcgagatct gggactggcg taaccatgac atcgacgcgc tggagtcgct gcgtggcgag
      421 ggcgtcgtct tcacgtcgat gaccggctac acgaccggca cgctcggcga ccccgagggg
      481 gcggaggagt tgctgcgcac cgcagcggag tcgattccgg tcggcaagcg gatcggcgcg
      541 tgggcgctaa acctgcacgg caccggcctg gacgacaaag gcctgcccgt caaggcgctc
      601 gcagccggcg aaatagcgag cgaggagatg tgggcgaccg cgcggcatac cctgcagcgc
      661 atcgccgaac tcggtgagcg cgagggcgtc accttcgtcc tcgaaaacct caacctcgcc
      721 gtcgaccacc ccggcacgcc gttcgcccgt gcggaggaca ctctcgcctt ggtgagcggc
      781 gtcgacagcc cgcacctgaa gctaaacctc gacctctatc acgcgcagat cggcgaggga
      841 aacctcatcg agttgtgccg ggcggcgctg ccgtggatcg gcgagatcca ggtcgccgac
      901 gtccctggcc gctgcgaacc gggcaccggc gagatcaact acggcggggt cgcccgggcg
      961 ctgcgcgaga tggggtatgc cggtgtggtc ggcctggaag ccttcgcctc cagcgactcc
     1021 gacctggccc ttcggcgctt ccgcgaggca ttcagcctcg cctgacccca tcgcgctgga
     1081 gcgcagcgcc gatccgacgc ctcggcgagt cccgtagcat gggtgctacg ctggtgctta
     1141 tgagtcagaa tgccgcaccc gctgagaccc tgtcgcagcg tgaattgcgc aatgagtccg
     1201 gccgggtgct gcgctccgtc gccgaaggcc ggtcctatgt cttgaccaat ggtggggttc
     1261 ctgtcgggcg aatcgtgccc atcgatgctc ccagcccggg actcaccatc gcccgggccg
     1321 cccgccggcg cggagggtgg gcggccttag ggattagacg caagcacagc gaccgctcgc
     1381 tggcggagct gctcgatgac ctgcgcgagg accgcgtgtg agcccgccgc cggttaccta
     1441 tgtcgacacc tcggcactgg gagccctcct gatcgagcaa ccggagagtg aagctctcgc
     1501 ggagtggctg gataacacca cggaccacct cgtctcctgc gacctcctcg aaaccgagct
     1561 gcgtcgactg gctgtgcgag aaggcctcga ccagcacgat gtcacggcga tcctcgacgg
     1621 cgttgcactc gcggcgttgg atcgcgcggc ctatcgcgga gcaggcatcc tgccgatgcc
     1681 ctatttgcgc gcgcttgacg ccctccacct cgaggctgcc ctgcgcctcg ctgctcactc
     1741 cgtgctgacc tacaaccacc ggctcgcggc ggcagccagg accctgggtc tgaccgttgt
     1801 caatcctgga cgcccagacg agcctcgctg actgtcacga cctggcggcc gactcacgca
     1861 cgggcagccc ggcggcggtg tatgacgcgt cgaccagggc catattgcgc acggcgaact
     1921 cggcgtccgt gacgacgggg gcgccgccgc gcacggctgc cgcgaaggcc tccagctggt
     1981 aggtgtagct ggagcggttg cccaggtgct cgacgacatc gtcctcgcgc tcgctggtca
     2041 ggtcggcggc ttcgtcgggc cggcggcggg cgcgtggcgc tgcagccgta tgccgcagga
     2101 tcagccggtc gtcctggtgc ggcaggggga agctcgggat gacgagctca ccctcgctgc
     2161 cggtgaccac gagggtgaac gaccagtcgt cggcgtccat atccgagcca ccggaacccg
     2221 ttgccccaga gggaaagtcg cccgaaacga agaggtggga gtcgaccccg ggatgcccgt
     2281 cgcgctcgac ggcgtccgcc tcccggatcg tcagcgagcc gccgcagaag cgctcgccga
     2341 ggagggcaag acaggacagc gagtagcagc cgagatccat cgtcgaccca ccggccaggt
     2401 ccaggcgcca acgcggatcg tccgcgccgg gatcgggcat gagcaacggc acgtcgatgt
     2461 ggcgaacctc gccgatggcg ccctcctcga taagcgcgac cgcgcgctgg aaaagcgggt
     2521 ggtagggata gtggaaggcc tccatcagta ctatctggcc ttctctcctc ctcgcctcgc
     2581 cggattcgct cgtccctcgc gtccccgggt cggcgcattg tcgagaaggc gtcacgccgg
     2641 aggcgcgcac ggcgtcggcg acctcgcgcg cctcggcggc gttggccgcc gagggctttt
     2701 ccgcgaggac gtgcttgccg gcagcggcga tcttggcgtt ccacggggcg tgcaggccgt
     2761 tggcaagtgg gttgtagacc gcctcgatct ccgggtcggc gagcagggcg tcatacgact
     2821 ccacgacgcg ctcgacgcca ttggcggcgg cgaaatcggc tgcgcgagaa cgatcgcgag
     2881 cggcaaccgc caccaggcgc gtgccggtcg cctgagccgg gctgacgatc gacaacggcg
     2941 cgatgcgggc agcgccgagg atgccgatcc gcagcggctc gacctcgggc aggacggtca
     3001 cagcgcctgc tcggcggcga gcatgttgcg agcggcgaac tccaggcctt cgagctgacc
     3061 gagctcggcg tcctcgtgct cgatattgca gggcaggtcg gggtcgatct cctgcagcgt
     3121 gcgcaggaac tcggtccaga acgcgacgtc gtggcccttg ccgatcgcga cgaagtccca
     3181 cgaggagttg ctcggccagc cggagagagt ggtgccgccg ccgagcggca ggtagccctt
     3241 ctccccctcg gcgacgcggg tgaagcgatc gtcgaggacg ccgttgatct tggcggcct
//
LOCUS       DAOTFI010000366         3291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_34369_length_3291_cov_7.185414, whole genome shotgun sequence.
ACCESSION   DAOTFI010000366 DAOTFI010000000
VERSION     DAOTFI010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34369_length_3291_cov_7.185414"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="P5558_23135"
     CDS             complement(<1..204)
                     /locus_tag="P5558_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27278.1"
                     /translation="MRLAVLALLAALPGIALADPLPSWNETDAKARIIAFVDGVTDTD
                     SDTYVTPADRIAVFDNDGTLWGEQ"
     gene            complement(259..1857)
                     /locus_tag="P5558_23140"
     CDS             complement(259..1857)
                     /locus_tag="P5558_23140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRY27279.1"
                     /translation="MRRSKTAPLMFAVGMTLAAVPAAGWGQDAQPAAGNQPNILVIWG
                     DDIGVHNISAYNHGIMGYQTPNIDRIASEGALFTDAYAQQSCTAGRASFILGQHPFRT
                     GLLTIGMPGSDHGIPEWSPTIADMLKDRGYTTGQYGKNHLGDRDEHLPTNHGFDEFFG
                     NLYHLNAEEEPESYYYPKDPAFREQFGPRGVIRASADGDIEDTGPLTRERMETVDEEI
                     IAGAVDFIERANEQNEPFFLWLNTTRMHVWTHLKEESRGTTGIGLYPDGMVEHDGMVG
                     QVLDKLEELGIADNTIVVYSTDNGAETVTWPDGGTTPFHGEKGTTWEGGFRVPLLVKW
                     PGVIEPGRKVNGIVSQEDWFPTFAAAAGEPDIVEKLKDGHTMNGKDWKVHLDGYNLLP
                     FLSGEAEASPRQEIFYFDQAGNLNAVRVQDWKVHFAILNGNITDAVRDVTAWPKVINL
                     KADPYEKAWEESGMYLRWYAENTMWTFVPVQQKIKEFFSTFEDYPYQLGSSLTASNLG
                     YNTLRNAELLKRLGGEDYQPGQRQ"
     gene            complement(1929..3092)
                     /locus_tag="P5558_23145"
     CDS             complement(1929..3092)
                     /locus_tag="P5558_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27280.1"
                     /translation="MSIDRLAGVVAAVALMGSLAGCATGPSGLGEAAGRSVDGVAATE
                     DLTRPPPDRGPGFGRRPAGAVQSADYTGDPADWEAGFHTADLRDDGPWQGIGSGWRLA
                     QADASPSSAPAGEGGGGGGVDLASANQAFANPLAQATLAIIENDTVTLGGDLSDGNRR
                     TNVTVFEPLIPVPLGNSGWSLVNRPILPIGFGADVPVAGGGGGPTTGVGSPQFDGKNG
                     LGDFTIFSLLTPTKGDSPIKWGIGPIFRFPTATDDQLGAEKFSLGPAAVALYSSKNFT
                     IGALNQNLFSVAGDGDRDDVRVSTLQYFAFYNFTPEWGIGTAPIISVDWDEAGNADGY
                     AVPVGLGVTRTFRIGEIPSRMLVEAQRYVVQKESFGPEWNFRVALAMFFPLLF"
BASE COUNT          505 a         1074 c         1135 g          577 t
ORIGIN      
        1 ctgctcgccc cagagcgtgc cgtcattgtc gaagacggcg atgcggtcgg ccggggtcac
       61 gtaggtgtcg gagtctgtgt cggtgacgcc gtcgacgaac gcgatgatcc gcgccttggc
      121 gtcggtctcg ttccacgagg gcaaggggtc ggccagggcg atgcccggca gcgccgcgag
      181 cagtgccaga acggcaagac gcatggatag ccctttcgag aggtagcggg cacgcccgag
      241 ggcgtgcccg caaggcgact actgacgctg gcccggctgg tagtcctcgc caccgagccg
      301 cttgagcagc tcggcgttgc gcagggtgtt gtagccgagg ttcgacgcgg tgagcgagga
      361 cccgagctgg tacgggtagt cctcgaaggt ggagaagaac tccttgatct tctgctggac
      421 gggcacgaac gtccacatcg tgttctcggc gtaccagcgg agatacatgc ccgactcctc
      481 ccacgccttc tcgtaagggt cggccttgag gttgatcacc ttgggccagg cggtcacgtc
      541 gcgcaccgcg tcggtgatgt tgccgttgag gatcgcgaag tgcaccttcc agtcctggac
      601 gcgcacggcg ttcaggtttc cggcctggtc gaagtagaag atctcctgtc gcgggctcgc
      661 ctcggcctcg cccgaaagga acggcagcag gttgtagccg tcgagatgga ccttccagtc
      721 cttgccgttc atggtgtggc cgtccttgag cttctcgacg atgtcgggct cgcctgcggc
      781 cgccgcgaag gtcgggaacc agtcctcctg gctgacgatc ccgttgacct tccggccggg
      841 ctcgatcacg cccggccact tgaccagcag aggcacgcgg aagccgccct cccaggtcgt
      901 gcccttctcg ccatgaaacg gcgtggtgcc gccgtcgggc caggtgaccg tctcggcgcc
      961 attgtcggtc gagtacacga cgatcgtgtt gtcggcgatg ccgagctcct cgagcttgtc
     1021 gagcacctgc ccgaccatgc cgtcgtgctc gaccatgccg tccgggtaga ggccgatgcc
     1081 cgtcgtgccc cgcgattcct ccttgaggtg cgtccagacg tgcatgcgcg tcgtgttcag
     1141 ccagaggaag aacggctcgt tctgctcatt ggcgcgctcg atgaaatcga cggccccggc
     1201 gatgatctcc tcgtcgaccg tctccatgcg ctcccgggtg agtgggccgg tatcctcgat
     1261 atcgccgtcg gccgaggcgc ggatcacccc gcgcgggccg aactgctcgc ggaaggcggg
     1321 gtccttgggg tagtagtagc tctccggctc ctcctcggcg ttcaggtgat agaggttgcc
     1381 gaagaactcg tcgaagccgt ggttcgtcgg caggtgctcg tcccgatcgc ccaggtggtt
     1441 cttgccgtac tggcccgtcg tgtagccgcg atccttgagc atgtccgcga tggtcggcga
     1501 ccattccgga atcccgtggt cggagcccgg catgccgatg gtcaggagcc ccgtccggaa
     1561 gggatgctgg ccgaggatga acgacgcccg gccggcggtg caggactgct gtgcgtaggc
     1621 gtccgtgaag agcgcgccct cactggcgat gcggtcgata ttgggcgtct ggtagcccat
     1681 gatgccatga ttgtaggcgc tgatgttgtg gacgccgata tcatcacccc agatcacgag
     1741 gatgttcggc tggttcccgg ctgccggctg ggcatcctgg ccccagccgg cggccggcac
     1801 ggcggcgagc gtcatgccca ccgcgaacat caatggtgca gtctttgatc ttctcaaggc
     1861 tttttttcct cctgtcgacc tggaatgctg gctggcgagc gctgccaact caagcaacgc
     1921 gcgtctggct agaagagcag cggaaagaac atggccagcg ccacgcggaa gttccactcc
     1981 ggcccgaagc tctccttctg gacgacgtag cgttgggcct cgaccagcat gcgtgacgga
     2041 atctcgccga tgcggaaggt tcgcgtcacg ccgagcccga ccggcacggc gtagccgtcg
     2101 gcgttgccgg cctcgtccca gtcgacggag atgatgggtg ccgtgccgat gccccactcg
     2161 ggcgtgaagt tgtagaaggc gaaatactgc agcgtggaga cgcggacatc gtcgcggtcg
     2221 ccgtcgcccg ccaccgagaa gaggttctgg ttgagggcgc caatggtgaa gttcttggac
     2281 gagtacaagg cgacggccgc cggcccgagc gagaacttct cggcgccgag ctggtcatcg
     2341 gtggcggtgg ggaagcgaaa gatcgggccg atgccccatt tgatgggcga gtcgcccttg
     2401 gtcggcgtca ggagcgagaa gatcgtgaag tcacccaggc cgttcttgcc gtcgaactgc
     2461 ggagagccca cgccggtcgt cgggcctccg ccgccgcccg ccacagggac atccgcgccg
     2521 aagccgatcg gcaggatcgg gcggttgacg agagaccagc cgctgttgcc gagagggacc
     2581 ggaatgaggg gctcgaagac cgtgacgttg gtccgtcgat tgccgtccga cagatcgccg
     2641 ccgagggtga ccgtgtcgtt ctcgatgatg gccagcgtcg cctgggccag ggggttggcg
     2701 aacgcctggt tggcggaggc gagatcgacc ccgccacccc caccaccttc gcccgccggg
     2761 gccgacgatg gcgatgcgtc cgcctgggcc agccgccagc cggagccgat gccttgccac
     2821 ggcccgtcat cgcgcaggtc cgcggtgtga aagccggcct cccagtcggc cggatcacct
     2881 gtgtagtcgg ccgactgcac ggcgcccgcc ggccgccggc cgaagcccgg gccccgatcg
     2941 ggaggcgggc gcgtgaggtc ttccgtggcc gcgacgccgt cgaccgagcg gccggcagcc
     3001 tcgccgaggc cggacgggcc ggttgcgcaa ccggcgaggc ttcccatcag cgccacggcg
     3061 gcgactaccc ccgccagtcg atcaatgctc atcgccaccg gtcctgctcc ccatttcaac
     3121 gaccacgttt tgccactcac cggaggcgag gccggccgaa ttgcattcga ccggcctcgg
     3181 gctccggaga gttggcgtcc agacctcggg ctacctgctg cccggctggt tcagcctctc
     3241 catcacctgg tcgagcgaga agctcgccgc cttctggcgc ggcgggaact g
//
LOCUS       DAOTFI010000367         3284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_34462_length_3284_cov_5.366987, whole genome shotgun sequence.
ACCESSION   DAOTFI010000367 DAOTFI010000000
VERSION     DAOTFI010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34462_length_3284_cov_5.366987"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1162)
                     /locus_tag="P5558_23150"
     CDS             complement(233..1162)
                     /locus_tag="P5558_23150"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY27281.1"
                     /translation="MATSTEHLKTGVYPIAPTPFHANGDLDLDGQKRVLDCMIDQGVD
                     GICILANYSEQFLLSDAERNTLLTLCLDHVAGRVPVIATTSHYSTRIAVERAKAAEAA
                     GAAMLMLMPPYHGALLKADEKGNFEHFEAIAGAVAIPIMVQDAPLSGVTLSVPFLTRL
                     AKELPTVAWFKIETPMAAGKLRALIAAGGDTIQGPFDGEESITLMADLDAGATGTMPS
                     ALLPDLIKPVVTFHLAGKAEEAAAQYAKILPLINFENRQCGLRATKTVMMAGGVIRSD
                     AVRHPFEPLHPATRDGLLQLARRTGPLAMRWGK"
     gene            1315..2115
                     /locus_tag="P5558_23155"
     CDS             1315..2115
                     /locus_tag="P5558_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY27282.1"
                     /translation="MRLSEAQLAEFASEGWLFLPGCFSAEEIGLLNAEAEGIYASGRE
                     EVWRESTGAPRTAFAAHTYNEAFGLLGRHPRLVEPVSQLFGEPVYMHQFKINAKAPFD
                     GEVWQWHQDYGTWARDDGMPEPRAMNIAVFLDEVMAVNGPLMFIPKSHRKGVLAAGHD
                     TATTSYPLWTLDKATITELVEEGGIVAPTGKPGSVLMFHGNLVHASPPNITPYPRKIV
                     YLTLCAVSNHIRKPTRPEWIAHRDFAPIEPCAEDALEVLARSRPMAAE"
     gene            complement(2278..2571)
                     /locus_tag="P5558_23160"
     CDS             complement(2278..2571)
                     /locus_tag="P5558_23160"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRY27283.1"
                     /translation="MQVSLTPELEAIVRRKLASGLYQNEGEVIREALRLMAARDRTAD
                     AKLQLLRDAIAAGEASGPVVELDLDDLSARLDKEASRAGLTRRAHAGCCDSVR"
     gene            complement(2662..3012)
                     /locus_tag="P5558_23165"
     CDS             complement(2662..3012)
                     /locus_tag="P5558_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRY27284.1"
                     /translation="MRTDPIEQLRAICLALPEATEKEAWGDPTFRVKDKIFAMPKEGD
                     GRLSVWFKAPPGCQEILVGAAPGTFFRPPYVGHKGWVGMRLDDGPDWTEVETLVRRSY
                     LLTAPKRLASLMPE"
     gene            3100..>3284
                     /locus_tag="P5558_23170"
     CDS             3100..>3284
                     /locus_tag="P5558_23170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27285.1"
                     /translation="MRLVFVLVLLLSGCAKVSEMHGPDGETRYLVGCYGALTPISVCQ
                     KKALELCPGGYTVDSSR"
BASE COUNT          481 a         1003 c         1267 g          533 t
ORIGIN      
        1 gggccgaaga cgagatcgcc cacgaccatg ccgaggatga aggtggtgac gacgagctgg
       61 gtggcgagcg ggtcggcgac gccgaggtcg tggccgatct ggcgcagcgc cggcagcacg
      121 gtgtcgatgc tggcggcggt gagcgagctc accagcgcga agagggcgac gaactcgacc
      181 ggccccggcc cggcggtcgt ggccggggcc gctcgcgctt cggtgggcgt cgctacttcc
      241 cccagcgcat ggcgagcggc ccggtgcggc gggcgagctg gaggaggccg tcacgggtgg
      301 ccgggtggag cggctcgaag gggtggcgga cggcgtccga cctgatcacg ccgccggcca
      361 tcatgacggt cttggtggcc ctgaggccgc attgccggtt ctcgaaattg atgagcggga
      421 ggatcttggc gtactgcgcc gccgcctcct cggccttgcc ggcgaggtgg aaggtgacga
      481 ccggcttgat caggtcgggg agcagggccg agggcatcgt gccggtcgct ccggcgtcga
      541 ggtcggccat cagcgtgatg gattcctcgc cgtcgaaggg gccctggatg gtgtcgccgc
      601 cggcggcgat cagcgcgcgg agcttgcccg cggccatggg cgtctcgatc ttgaaccagg
      661 cgacggtcgg caactccttc gccaagcgcg tgaggaacgg gacggagagg gtgacgccgg
      721 agagcggggc gtcctggacc atgatgggga tggcgacggc cccggcgatc gcctcgaaat
      781 gctcgaaatt gcccttctcg tcggccttga ggagggcgcc gtgatagggc ggcatgagca
      841 tcagcatggc ggccccggcg gcctcggcgg ccttcgcgcg ctcgacggcg atgcgggtcg
      901 agtagtggga ggtggtggcg atgacgggga cacggccggc gacgtggtcg aggcagaggg
      961 tgaggagggt gttgcgctcc gcgtcggaga gcaggaactg ctcggagtag ttggcgagga
     1021 tgcagatgcc gtcgacgccc tggtcgatca tgcagtcgag gacgcgcttc tgcccgtcga
     1081 ggtcgaggtc gccattggcg tggaaggggg tgggggcgat cgggtagacg ccggtcttca
     1141 ggtgctcggt cgacgtggcc atggtttcct ccctcggctg gcgcggcgga gagtgcgccg
     1201 gccgggccgg cggatcaagt gccgggcttg gcggtgccga ggctttgcgg gggatggcgg
     1261 gggcggtaga atggtggccc gtcacgggga cggggtctcg agggaggatt ggtcatgcgt
     1321 ctgagcgaag cgcagctcgc cgagttcgcg agcgagggct ggctcttcct gccgggctgc
     1381 ttctcggcgg aagagatcgg cctgctcaat gccgaggcgg agggcattta tgcgtcgggg
     1441 cgcgaggagg tctggcgcga gagcacaggg gctccccgga cggccttcgc ggcgcacacc
     1501 tacaacgagg cgttcggcct gctggggcgg catccgcggc tggtggagcc ggtgagccag
     1561 ctcttcggcg agccggtcta catgcaccag ttcaagatca acgccaaggc acccttcgac
     1621 ggcgaggtct ggcagtggca ccaggattac ggcacctggg cccgggacga cggcatgccg
     1681 gagccgcggg cgatgaacat cgccgtcttc ctcgacgagg tgatggcggt gaacgggccg
     1741 ctgatgttca tccccaagag ccaccgcaag ggcgtgctcg ccgccgggca cgacacggcg
     1801 acgacgtcct atcccttgtg gacgctggac aaggcgacga tcacggagct ggtcgaggag
     1861 ggcgggatcg ttgcaccgac cggcaagccc ggctcggttt tgatgttcca cggcaacctc
     1921 gtgcatgcct cgccgcccaa catcacgccc tatcccagga agatcgtcta cctgacgctc
     1981 tgcgcggtct cgaaccatat caggaagccg acgcggccgg agtggatcgc gcatcgggac
     2041 tttgcgccga tcgagccgtg cgcggaggac gcgctggagg tgctcgcgcg gtcgcggccg
     2101 atggcggcgg agtagggcgc ggcttgcgga ggcgaacagg cgtcgaatct cggagggatg
     2161 cggttcgcca tcccgcaacg tcgcatcgcg tcgttcctca ggttgtgtgc cccgaggcga
     2221 gcgatcgctg cgctcctctc gatccgaggc atgcgatcat gtcgacggcc gaggcaatca
     2281 tcgaacgcta tcgcaacacc ctgcgtgcgc tcgccgagtg aggccggctc gactcgcttc
     2341 tttgtccagc cgtgccgaaa ggtcgtcgag gtcgagttcg acgaccggcc cgctggcctc
     2401 gccggccgcg atggcgtcgc gcaggagctg cagcttcgcg tcggcggtgc ggtcgcgtgc
     2461 cgccatcagc cgaagcgcct cgcggatcac ctcgccctcg ttctggtaga ggccggacgc
     2521 cagtttccgg cggacaatgg cttccagttc cggcgtgagg gagacctgca tcgatgacgt
     2581 tcccaggctg tgcgcgtccc catcatcgta gccgatcggg tcggcggcag gaaggatcgg
     2641 gggcggccgg gctggcgccg tctactccgg catgagtgag gccaggcgct tcggggccgt
     2701 gagcaggtag ctgcggcgga cgagggtttc gacctcggtc cagtcggggc cgtcatcgag
     2761 gcgcatgccg acccagccct tgtggccgac atagggcggg cggaagaagg tcccgggggc
     2821 ggcgccgacg aggatctcct ggcagccggg cggggccttg aaccagacgg agaggcggcc
     2881 gtcgccctct ttcggcatgg cgaagatctt gtctttcacc cggaaggtcg gatcgcccca
     2941 ggcttccttc tcggtcgcct cgggcaaagc gaggcagatg gcgcggagct gctcgatcgg
     3001 gtcggtcctc atgctggctc ttccttcctt ggccggcttg tccgccggcg gcaaagccgc
     3061 tagggcgatg gcagctccat catagcggat cccggtgcca tgcggctcgt cttcgtcctg
     3121 gttcttcttc tgagcggctg tgccaaggtc agcgagatgc acgggccgga tggcgagacc
     3181 cgctacctgg tcggctgcta tggcgcgctg acgccgatca gcgtctgcca gaagaaggcc
     3241 ctggagctct gcccgggtgg ctacacggtg gacagctcgc gcga
//
LOCUS       DAOTFI010000368         3270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_34658_length_3270_cov_5.456921, whole genome shotgun sequence.
ACCESSION   DAOTFI010000368 DAOTFI010000000
VERSION     DAOTFI010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34658_length_3270_cov_5.456921"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="P5558_23175"
     CDS             complement(<1..314)
                     /locus_tag="P5558_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY27286.1"
                     /translation="MSAATMQPASLEIVGATKSFPGVMALDDVSINVDAGSFHALLGE
                     NGAGKSTLVKCIMGFYSGDKGQVLLDGREVSIRSPRDARALGIGMVYQHFTLVPSLTA
                     AE"
     gene            complement(316..1296)
                     /locus_tag="P5558_23180"
     CDS             complement(316..1296)
                     /locus_tag="P5558_23180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27287.1"
                     /translation="MASGSLAGQRNRFARWRGLAPVGVLVLLCVVITVISPNFLSFSN
                     LVRLLSGAAIPLVLVMGATFVILMGSIDLSVEGVVALAAVITSMLVLNDFTSADLGLL
                     AVPVAVVAGGCIGFVNGFVHVRLKIPSFMATLGFGFAGVGVATAILGGFTVRIADRGF
                     RELALGRFLDVPFSVWLAGMAVLVAWVIQERTRLGRWVYAIGTDEVTAKHAGVPVERT
                     RILVFTLAGLFYGLGGALAAAQLGQGHALMGTGRLFTTITAVVVGGTALSGGVGSVLN
                     SVIGVGIVIVLTNGMVLMGIPPYVQQGVQGLMIIVAVALALDRSRLVVVK"
     gene            complement(1366..2463)
                     /locus_tag="P5558_23185"
     CDS             complement(1366..2463)
                     /locus_tag="P5558_23185"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY27288.1"
                     /translation="MIHKRLGPIARRQLLRRDLLKGAAGFAGAGVLAGTFPRLAAAQS
                     GEWTLAISMRSLANPYHATFAAGGEAYAESVGAPFEVLITEGNSEKGMADIRALLART
                     GGKLVLNVDPNDSPDARVIVEECQRAGAYVVTQWNKPDDLHPWDFDPNYVAHMSFDGV
                     PTAQAVAEALFEAMGGEGGIVALGGILSNVPAIERKIGLDNAIAATNGKVQLLDFQPA
                     DWNETRAFEIMQAWLTRFGDDIKGVFAANDGMAIGALEALRQEGKAGAVPVTGIDGIE
                     AALFAIEAGEMAGTVAWDPNWTGGIGLALGHAVALGEIDIAAEPHEHREFYGTGIVVT
                     PANLAEYRAMDAALDFGDFWGKSPGQIQYRG"
     gene            complement(2551..>3270)
                     /locus_tag="P5558_23190"
     CDS             complement(2551..>3270)
                     /locus_tag="P5558_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRY27289.1"
                     /translation="HFGIDIVYELKGPWSLDKYQKPHWMLWAGLEYLLFKRGPVTSNI
                     VEGGAFWYADKAAETPDLQFHFLAGAGVEAGVPVISSGSGVTLNSYTLRPESRGSVTL
                     RSAEPKDKPVVDPNFLAEASDLQTSLEGLRLSREMMHQPALAPYVRGEHFPGEAVRTK
                     AELEAYARRYGRTSYHPVGTCRMGVDEMAVVDPELRLRGIEGLRVCDSSIMPRLVSSN
                     TNAPSIMIGEKASDLIRGNRA"
BASE COUNT          549 a         1174 c         1080 g          467 t
ORIGIN      
        1 ttctcggcgg ccgtcagaga cggcaccagc gtgaagtgct ggtagaccat gccgatgccg
       61 agcgcgcgcg cgtcgcgcgg cgagcggatc gacacctccc gcccatcgag gagcacctgt
      121 cccttatccc cgctgtagaa gcccatgata catttcacca gcgtcgactt gccggccccg
      181 ttctcgccga gcagcgcatg gaacgaaccg gcgtcaacat tgatggagac gtcgtcgagc
      241 gccatgaccc ccggaaagct cttggtggcg ccgacgatct cgaggctggc cggctgcatc
      301 gtcgcggcgc tcatctcact tcaccaccac gagcctggag cgatcgaggg ccagcgccac
      361 cgcgacgatg atcatcagcc cctgcacccc ctgctgcacg tagggcggga tacccatcag
      421 caccatgccg ttggtgagca cgatgacgat gccgaccccg atcaccgagt tcagcaccga
      481 gccgaccccg cccgagagcg ccgtgccgcc gacgacgacg gcggtgatcg tggtgaagag
      541 gcggcctgtc cccatcagcg catggccctg gccgagctgg gccgcggcga gcgccccgcc
      601 gagcccgtaa aacagccccg ccagggtgaa gacgaggatg cgcgtgcgct cgaccggcac
      661 cccggcatgc ttcgccgtca cctcgtccgt gccgatggcg tagacccagc ggccgagcct
      721 cgtccgctcc tggatcaccc aggcgaccag cacggccatg cccgccagcc agacgctgaa
      781 cggcacgtcg aggaagcgcc cgagcgccag ctcgcgaaag ccccggtcgg cgatccgcac
      841 ggtgaagccg ccgaggatcg ccgtggcgac accgaccccg gcgaagccga agccgagcgt
      901 cgccatgaag ctcggaatct tcagccggac atggacgaag ccgttgacga agccgatgca
      961 gccgccggcc acgaccgcca ccggcacggc cagaagcccg agatcggcgc tcgtgaagtc
     1021 gttcagcacc agcatgctgg tgatgacggc ggcgagcgcc acgacaccct cgaccgagag
     1081 gtcgatgctg cccatcagga tgacgaaggt cgcccccatc accaggacca gcggaatggc
     1141 ggccccgctc agcagcctga cgaggttcga gaaggagagg aagttcgggc tgatgacggt
     1201 gatgacgacg cagagcagca cgagcacgcc gaccggcgcc agcccgcgcc agcgggcgaa
     1261 gcggttgcgc tggccggcga ggctaccgga cgccatcagc ccgttctcca gggatgcgag
     1321 aagagcagcg cgggccgggc aacatcccgg gcccgcgccg ccagctcagc cgcgatactg
     1381 gatctggccc ggcgacttgc cccagaaatc gccgaagtcg agggcggcat ccatcgcccg
     1441 gtactcggcg aggttggccg gggtgacgac gatgccggtg ccgtagaact cgcgatgctc
     1501 gtgcggctcg gcggcgatgt cgatctcgcc cagcgccacc gcatggccca gcgccaggcc
     1561 gatgccgccg gtccagttcg ggtcccaggc caccgtgccg gccatctcgc ccgcctcgat
     1621 ggcgaagagc gccgcctcga tgccgtcgat gccggtcacc ggcacggccc cggccttgcc
     1681 ctcctggcgg agcgcctcga gggcgccgat cgccatgccg tcattggcgg cgaagacccc
     1741 cttgatgtcg tcgccgaagc gggtgagcca ggcctgcatg atctcgaagg ccctggtctc
     1801 gttccagtcg gccggctgga agtcgaggag ctgcaccttg ccgttggtcg ccgcgatcgc
     1861 gttgtcgagc ccgatcttgc gctcgatcgc cgggacgttg gagaggatgc cgcccaaggc
     1921 gacgatgccg ccctcgccac ccatcgcctc gaacagcgcc tcggccaccg cctgcgccgt
     1981 cggcacgccg tcgaacgaca tgtgcgccac gtagttgggg tcgaagtccc agggatgcag
     2041 gtcgtccggc ttgttccact gggtcaccac ataggcgccg gcgcgctggc actcctcgac
     2101 gatcacccgc gcatcgggcg aatcgttcgg gtcgacgttc agcacgagct tgccgccggt
     2161 ccgcgcgagc agggcccgga tgtccgccat gcccttctcg gaattgccct cggtgatcag
     2221 cacctcgaaa ggggctccca ccgactcggc ataggcctcg ccgcctgccg cgaaggtggc
     2281 gtgatagggg ttggcgagcg agcgcatgga gatcgccagc gtccactcgc ccgactgcgc
     2341 cgcggcgagc cgcgggaacg tgccggcgag cacgcccgca ccggcgaaac ctgccgcgcc
     2401 cttgaggaga tcacggcgca aaagctgccg cctggcgatg gggccgagcc gtttatggat
     2461 catttccgcc tccctggatt gtgcccgtta acgcactaaa cggttacttc taggccgaag
     2521 ccggccacgg cgcaattcaa aaccggttcg tcaggcccgg ttgccgcgga tcaggtccga
     2581 cgccttctcg ccgatcatga tcgagggcgc gttggtgttg gagctgacca gccgcggcat
     2641 gatcgagctg tcgcagaccc gaagcccctc gatgccgcgc agccgaagct cggggtcgac
     2701 caccgccatc tcgtccacgc ccatccggca ggtgccgacc ggatggtacg acgtccgccc
     2761 gtagcgccgg gcataggctt cgagctccgc cttcgtgcgc accgcctcgc ccgggaagtg
     2821 ctcgccccgg acatacgggg cgagcgccgg ctggtgcatc atctcgcgcg agagcctgag
     2881 cccctcgagc gaggtctgca ggtcagaggc ttcggcgagg aaattcgggt ccaccaccgg
     2941 cttgtctttc ggctcggcgc tcctgagcgt caccgagccc cggctctccg gccggagcgt
     3001 gtaggagttg agcgtgacgc cggagcccga ggagatcacc ggcacccccg cctcgacccc
     3061 cgccccggcg aggaagtgga actgcaggtc cggcgtctcc gccgccttgt ccgcatacca
     3121 gaaggccccg ccctcgacga tgttggaggt caccggcccg cgcttgaaga gcaggtactc
     3181 gagccccgcc cagagcatcc agtgcggctt ctggtacttg tcgaggctcc agggcccctt
     3241 cagctcgtag acgatgtcga tgccgaaatg
//
LOCUS       DAOTFI010000369         3231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_35238_length_3231_cov_7.482053, whole genome shotgun sequence.
ACCESSION   DAOTFI010000369 DAOTFI010000000
VERSION     DAOTFI010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35238_length_3231_cov_7.482053"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="P5558_23195"
     CDS             <1..464
                     /locus_tag="P5558_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HRY27290.1"
                     /translation="DEPTTALDVTIQAQILDLLRELQEELRMAVVLITHDLGVVAQHV
                     DRVMVMYAGRVVEEASVMDAFARPSHPYTQGLLRSIPSLDEERERLDAIPGMVPSPFA
                     LPPGCRFAPRCIHARDACAAMHPPLIEVGEAHRAACIGLVDYDLARLAATA"
     gene            461..1441
                     /locus_tag="P5558_23200"
     CDS             461..1441
                     /locus_tag="P5558_23200"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRY27291.1"
                     /translation="MTALVEARGLTKHFPIRGRGLFARQTGVVRAVDGVDFAVGRGET
                     LGLVGESGCGKTTTGRMLVRLIEPTAGSVLFEGAPIEGLEPEAMKPIRREMQIIFQDP
                     YGSLNPRMKVAEIVAEPLEIHSVGTAAERRARVAELLGTVGLAAYQAERFPHEFSGGQ
                     RQRIGIARALALQPKLMVCDEPVSALDVSIQAQVVNLLQDLQQQLGLTYLFISHDLGV
                     VRHIADRVAVMYLGQIVEIAPKRDLYRRPQHPYTQALLEAIPVADPARRRRAQAIRGD
                     VPSPANPPAGCRFHTRCPHVMDVCRRIEPKLLETAPGHRAACHLLHQPSE"
     gene            1455..2495
                     /locus_tag="P5558_23205"
     CDS             1455..2495
                     /locus_tag="P5558_23205"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRY27292.1"
                     /translation="MSAQSRIACPLDFDKAGRQVGHLRLPHSSNTSAYGWIGIPIAVI
                     QNGSGPTVYLGGGNHGDEYEGQISLVRLIQELDPARIQGRLIIMPATNLPAAMNHSRC
                     SPIDGGNLNRSFPGEANAPDNRPTAAIAHYVEEVLLPMCDAMLDMHSGGKTLDYVPSG
                     LIRRDGPDDPLFARKLEALKAYGAPIGYVVEGGGMPAAGGGIAAATITCAADRKGVLG
                     MGTELGGAGTVTLDTLGVARRGIENFLAFMGVMEKPATADSPKSRLLKVAGPDYYVHA
                     PCRGAFEPAFRLGDEVAAGQVCGWIHQIDEPDQPSREVRFERAGVAFCKRPITAVEMG
                     DCLAHLGTDWQG"
     gene            2499..>3231
                     /locus_tag="P5558_23210"
     CDS             2499..>3231
                     /locus_tag="P5558_23210"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRY27293.1"
                     /translation="MVEKARSPLWTDVDYDAPGKQVGFLHLPHSVTRSAYGTIPIPLA
                     VIANGSGPTVLLMAGNHGDEYEGQAALCDFVRKVDPAEVQGRIIVLPATNLPAAVAGT
                     RTSPLDEGNLNRAFPGDPTDGPTGKIAHYLDTVLFPMCDATHDFHSGGASLDYLTLAT
                     MRLSGDADLDRRARAALDAFAPPLAMVWAFTNEPTLSAACALSHKLIALGGEFGGRGA
                     VRLEGIRVIEAGMRRFLDHLGVMEAP"
BASE COUNT          468 a         1196 c         1086 g          481 t
ORIGIN      
        1 ccgacgagcc gaccaccgcc ctcgacgtca ccatccaggc gcagatcctc gaccttctcc
       61 gcgagctgca ggaggagctc cgcatggccg tcgtgctgat cacccacgat ctcggcgtcg
      121 tggcgcagca tgtcgaccgg gtgatggtga tgtatgcggg ccgggtggtc gaggaggcgt
      181 cggtcatgga cgccttcgcc cggccgagcc atccctacac gcaggggctg ctccgcagca
      241 tcccctcgct cgacgaggag cgcgagcgtc tcgacgccat acccggcatg gtgccgagcc
      301 ccttcgccct gccgccgggc tgccgcttcg cgccgcgctg catccacgcc cgagatgctt
      361 gtgcggccat gcatccgccg ctcatcgagg tcggcgaggc gcatcgggcc gcctgcatcg
      421 ggctggtcga ctacgatctc gcccgcctgg cggccaccgc atgacggcgc tcgtcgaggc
      481 gcggggcctc accaagcact tcccgatccg cgggcgcggc ctcttcgcca ggcagacggg
      541 ggtggtgcgc gcggtcgacg gggtcgactt cgccgtcggc cggggcgaga ccctcggcct
      601 cgtcggcgaa tcgggctgcg gcaagaccac gacggggcgg atgctggtgc ggctgatcga
      661 gcctaccgcc ggcagcgtcc tcttcgaggg ggcgccgatc gaggggctgg agccggaggc
      721 gatgaagccg atccggcgcg agatgcagat catcttccag gatccctacg gctccttgaa
      781 cccgcgcatg aaggtggcgg agatcgtcgc cgagcctttg gagatccact cggtcggcac
      841 ggcggcggag cggcgcgccc gggtggccga gctgctcggc accgtcggcc tcgccgccta
      901 ccaggcggag cgcttcccgc acgagttctc gggcggccag cggcagcgga tcggcattgc
      961 ccgggccctg gcgctgcagc ccaaactcat ggtctgcgac gagccggtct ctgccctcga
     1021 cgtctcgatc caggcgcagg tcgtcaatct cctgcaggac ctgcagcagc agctcggcct
     1081 cacctacctc ttcatcagcc acgacctggg cgtcgttcgg cacatcgccg accgggtggc
     1141 ggtgatgtat ctgggccaga tcgtcgagat cgcccccaag cgcgacctct accgccggcc
     1201 gcagcatccc tacacccagg ccctgctcga ggcgatcccg gtggccgacc cggcgcggcg
     1261 ccggcgggcg caggcgatcc gcggcgacgt gccgagcccc gccaacccgc ccgccggctg
     1321 ccgcttccac acccgctgcc cgcacgtcat ggacgtctgt cgccgcatcg agccgaagct
     1381 gctggagacg gcgcccgggc accgggcggc ctgccatttg ctgcaccagc cttctgaatg
     1441 aggagtcttt cgtcatgagc gcccagagcc ggatcgcctg tcccctggat ttcgacaagg
     1501 ccggcaggca ggtggggcat ctgcggctgc cgcattccag caacacctcc gcctatggct
     1561 ggatcggcat ccccatcgcg gtgatccaga acggcagcgg gcccacggtc tatctcggcg
     1621 gcggcaatca cggcgacgag tacgagggcc agatctcgct cgtccggctg atccaggagc
     1681 tcgacccggc gcgcatccag ggccggctga tcatcatgcc ggcgaccaat ctgccggcgg
     1741 cgatgaacca cagccgctgc tcgccgatcg acggcggcaa cctcaaccgc agcttcccgg
     1801 gcgaggccaa cgccccggac aaccggccga ccgccgccat cgcgcattat gtcgaggagg
     1861 tgctgctgcc gatgtgcgac gccatgctcg acatgcattc gggcggcaag acgctggact
     1921 acgtgccgag cggcctgatc cgccgcgacg ggccggacga cccgctcttc gcccgcaagc
     1981 tcgaggccct gaaggcctat ggagcaccca tcggctatgt cgtcgagggc ggcggcatgc
     2041 cggccgccgg tgggggcatc gcggccgcca ccatcacctg tgcggccgac cgcaagggtg
     2101 tcctcggcat gggcacggaa ttgggcgggg ccggcacggt gacgctcgac acgctgggcg
     2161 tggcgcgccg cggcatcgag aacttcctgg cgttcatggg cgtcatggag aagccggcca
     2221 cggccgacag cccgaagtcc cggctcctca aggtcgccgg ccccgactac tacgtccacg
     2281 caccctgccg gggcgccttc gagccggcct ttcgcctcgg cgacgaggtc gcggccgggc
     2341 aggtctgcgg ctggatccac cagatcgacg agccggacca gccctcgcgc gaggtgcgct
     2401 tcgagcgggc cggcgtcgcc ttctgcaagc ggccgatcac cgccgtcgag atgggcgact
     2461 gcctcgccca tctcggcacc gactggcagg ggtagcgggt ggtggagaag gccaggtcac
     2521 cgctctggac ggatgtcgac tacgacgccc cgggcaagca ggtgggcttt ttgcacctgc
     2581 cgcattcggt cacccgctcg gcctacggca ccatccccat accgctcgcc gtcatcgcca
     2641 atggcagcgg ccctaccgtg ctgctcatgg ccggcaatca cggcgacgag tacgagggcc
     2701 aggccgcctt gtgcgacttc gtccgcaagg tcgatccggc cgaggtgcag ggccgcatca
     2761 tcgtgctgcc cgccaccaac ctgccggccg ccgtcgccgg cacccgcacc tcgccgctcg
     2821 acgagggcaa tctcaaccgc gcctttccgg gtgacccgac ggacgggccg acgggcaaga
     2881 tcgcccacta cctcgacacc gtgctcttcc cgatgtgcga tgcgacgcac gacttccatt
     2941 cgggcggcgc ctcgctcgac tacctgacgc tggccaccat gcgcctctcg ggcgacgccg
     3001 atctcgatcg ccgggcacgc gccgcgctgg atgccttcgc cccgccgctc gccatggtct
     3061 gggccttcac caacgagcca acgctctccg ccgcctgcgc cctctcccac aagctcatag
     3121 ctctgggcgg cgaattcggc ggtcggggtg ccgtccggct cgaggggatt cgggtgatcg
     3181 aggcgggcat gcgccgcttc ctcgatcatc tgggggtcat ggaagcaccc g
//
LOCUS       DAOTFI010000370         3229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_35265_length_3229_cov_4.835854, whole genome shotgun sequence.
ACCESSION   DAOTFI010000370 DAOTFI010000000
VERSION     DAOTFI010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35265_length_3229_cov_4.835854"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1145
                     /locus_tag="P5558_23215"
     CDS             129..1145
                     /locus_tag="P5558_23215"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY27294.1"
                     /translation="MRLPNLDRRRLLGSLAAGAAFVSLAPSARGQTRQIIVQYDWLMS
                     NGQIGDVVAQANGYFAEAGLEVELMPGGPNSTTVPPVTANQALVGQFSDSGQGMLARS
                     AGAPIKMIAAGFRQSPFAYFSLPAKPIRSVEDMRGKRIGTQPTARFVLDALLDKAGID
                     PGELEIVQVGWDMGPLANGQVDAITAWVTNTNALSILGDDRIALMQWDAGLPSYANVY
                     FTSERALAEDADTIAAFIGAVARGWGWMYENRAASVDLLVDAYDQLDRAVEQATVETI
                     VALAFDAATKEHGWGTFDPQVVADQIALYEKIGQFTGEVPTVEGFTTTAILDATEGER
                     PKLG"
     gene            1138..1917
                     /locus_tag="P5558_23220"
     CDS             1138..1917
                     /locus_tag="P5558_23220"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY27295.1"
                     /translation="MAEPFAVRMAGIGVVFERDGQRFEALRTVSLELAAGAFVCLIGP
                     SGCGKSTLLRVLADLVPIAGGEARILGQTPAEARAARRLGFCFQDATLLPWRTVIANV
                     RLALEVGRPAAARPPRRSPEELLELVGLGARLDALPGELSGGMRQRVAIARALVGDPA
                     LLLMDEPFGALDELTRDRLNDELLRIWQETGTTIVFVTHSLGEAVYLGQEVVVMAAGP
                     GRIHARLDLRPAKAEGGLGRDSATFAALTGELRSLLAEAGA"
     gene            1914..2669
                     /locus_tag="P5558_23225"
     CDS             1914..2669
                     /locus_tag="P5558_23225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27296.1"
                     /translation="MTRSGDAARGWLLPVLGFVSILVLWHAVVVLLQVPEFVAPTPLA
                     ALRTLGDRWPLLLQNFWPTAVEALAGFLLGNTLAVLTAVVFVHSRLVERAYFPVAVFF
                     NTIPVLALAPILVLIFGLGMLPKIVIAAVICYFPTLVNMIRGLEAVGRPELELMRVLN
                     ASRLEIFWRLRVPRSLPFLFAALRIASTTCVVGAIVGEWIGANNGLGALIIQATFNYQ
                     SPLLYATLFVSSGLALLFFGAVVALEKRVLRWS"
     gene            2690..3121
                     /locus_tag="P5558_23230"
     CDS             2690..3121
                     /locus_tag="P5558_23230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY27297.1"
                     /translation="MLGQSEKISDVCLICADLERSVAFYRDRLGFRLRRKAEGFADFA
                     SEGVTLALWEAGHIAAHVGIEGPPAARLGHKVMVAIQVTDAARVDALHAELIAAGVTP
                     HGPPRSYPWNAYACYFADPDGNLWEIYAWVGDPDGYHQTFT"
BASE COUNT          443 a         1214 c         1041 g          531 t
ORIGIN      
        1 gcgccaccac ggcggcgcgg gggccctcta cgtcctgctc aggcggctgc gctagcgcca
       61 cttgcagcgc ccccggcgga ctgtcatgct ggcgccgctc gacaagccaa gaaaagggaa
      121 aagaaccggt gcgcctcccc aacctcgacc ggcgtcgcct gctcggcagc ctcgccgccg
      181 gtgccgcctt cgtcagcctc gccccgtcag ccagaggcca gacccgccag atcatcgtcc
      241 agtacgactg gctgatgagc aacggccaga tcggcgatgt ggtggcccag gcgaacggct
      301 acttcgccga ggccgggctc gaggtcgagc tgatgccggg cggccccaac tccaccaccg
      361 tcccgccggt caccgccaac caggcgctgg tcggccagtt ctccgacagc ggccagggca
      421 tgctggcgcg ctcggcgggt gcccccatca agatgatcgc cgccggcttt cgccagtcgc
      481 ccttcgccta tttctcgctg ccggcgaagc ccatccgctc ggtcgaggac atgcgcggca
      541 agcgcatcgg cacacagccg accgcccgct tcgtcctaga cgccctcctc gacaaggcag
      601 ggatcgatcc cggcgagctc gagatcgtcc aggtcggctg ggacatgggt ccgctcgcca
      661 acggccaggt cgacgcgatc accgcctggg tcaccaacac caacgcgctc tccatcctgg
      721 gcgacgaccg gatcgcgttg atgcagtggg atgcgggcct gccgagctac gccaatgtct
      781 atttcacctc agagcgggcg ctggccgagg acgccgacac catcgccgcc ttcatcggtg
      841 cggtcgcccg cggctggggc tggatgtacg agaatcgcgc cgcctcggtc gatctcctgg
      901 tcgatgccta tgaccagctc gaccgcgccg tcgagcaggc gacggtcgag accatcgtcg
      961 ccctcgcctt cgacgcggcc acgaaggagc atggctgggg cactttcgat ccacaggtgg
     1021 tcgccgacca gatcgcgctc tatgaaaaga tcggccagtt caccggcgag gtgccgacgg
     1081 tcgagggctt cacgaccacg gccatcctgg acgccaccga aggggagcgg ccgaagcttg
     1141 gctgagccgt tcgccgtccg gatggccggc atcggcgtcg tcttcgagcg cgacggccag
     1201 cgcttcgagg cgctgcggac ggtctcgctc gagctcgccg ccggcgcctt cgtctgtctc
     1261 atcggaccct ccggctgcgg caagtcgacc ctgctccgcg tcctcgccga tctcgtgccc
     1321 atcgctggcg gcgaggcccg catcctcggc cagacaccgg ccgaggcccg ggccgcgcgg
     1381 cggctcggct tctgcttcca ggacgcgacg ctgctgccct ggcggaccgt gatcgccaat
     1441 gtccggctcg ccctcgaggt cggtaggccg gcggcggccc ggccacccag acggagcccc
     1501 gaggagcttc tggagctggt cggcctcggc gcccggctcg atgccttgcc gggcgagctc
     1561 tcaggcggca tgcgccagcg cgtcgcgatc gcccgcgccc tggtcggcga cccggccctc
     1621 ctcctcatgg acgagccctt cggcgccctc gacgagctga cccgcgaccg gctgaacgac
     1681 gagctgctgc gcatctggca ggagaccggc acgaccatcg tcttcgtcac ccacagcctc
     1741 ggcgaggccg tctatctcgg ccaggaggtc gtcgtcatgg ctgccggccc ggggcgcatc
     1801 catgcccggc tcgacctccg cccggccaag gcggagggcg gcctcgggcg cgacagcgcc
     1861 accttcgccg ccctcaccgg cgagctgcgc agtctcctgg cggaggccgg cgcatgacca
     1921 gatccggaga tgcggccagg ggctggctcc tgccggttct gggctttgtc tccatcctcg
     1981 tcctctggca cgccgtggtg gtgctgctgc aggtgccgga gttcgtcgcc ccgaccccgc
     2041 tcgcggcgct ccgcaccctg ggcgaccgct ggccgctcct tttgcagaac ttctggccga
     2101 cggcggtcga ggcgctcgcc ggcttcctcc tcggcaacac gctggccgtc ctgaccgccg
     2161 tggtcttcgt ccacagccgg ctggtcgagc gcgcctactt cccggtcgcc gtcttcttca
     2221 acaccatccc ggttttggcc ctggcgccga tcctggtcct catcttcggc ctcggcatgc
     2281 tgccgaagat cgtcatcgcc gcggtgatct gctactttcc gacgctggtg aacatgatcc
     2341 gcggcctcga ggccgtcggg cggcccgagc tcgagctgat gcgggttctc aatgccagcc
     2401 gcctcgagat cttctggcgg ctgcgcgtgc cgcgctcgct gcccttcctc ttcgccgccc
     2461 tgcgcatcgc atcgaccacc tgcgtggtcg gcgccatcgt cggcgagtgg atcggcgcga
     2521 acaacggcct cggcgccctg atcatccagg cgaccttcaa ctaccaatcg cccttgctct
     2581 atgcgacgct cttcgtctcg tcgggtctcg ccctcctctt cttcggcgcc gtcgtcgccc
     2641 tcgagaagcg cgtgctgcgc tggagctgaa tcccctttcg gagagagcca tgctgggcca
     2701 gagcgagaag atcagcgatg tctgcctgat ctgcgccgac ctcgagcgat ccgtcgcctt
     2761 ctaccgggac aggctcggct tcaggctgcg ccgcaaggcc gagggcttcg ccgatttcgc
     2821 cagcgagggc gtcaccctcg ccctgtggga ggccggccat atcgccgccc atgtcggcat
     2881 cgagggtcca cccgctgccc ggctggggca caaggtcatg gtggcgatcc aggtgacaga
     2941 cgccgcccgc gtcgacgcgc tccacgccga gctcatcgcc gccggcgtca ccccgcacgg
     3001 ccccccgcgt tcctatccct ggaacgccta cgcctgctac ttcgccgacc cggacggcaa
     3061 cctctgggaa atctatgcct gggtcggcga tccagacggt taccatcaaa ccttcacctg
     3121 agcccaatcg gctaggaggc gtgccgccga tccggtaggc tccctgcgag catcgcatcg
     3181 ggaggatcgg cgaatggtgg agggcgctcc gcaggccctg aaggtggac
//
LOCUS       DAOTFI010000371         3172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_36102_length_3172_cov_5.983638, whole genome shotgun sequence.
ACCESSION   DAOTFI010000371 DAOTFI010000000
VERSION     DAOTFI010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36102_length_3172_cov_5.983638"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="P5558_23235"
     CDS             <1..1746
                     /locus_tag="P5558_23235"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HRY27298.1"
                     /translation="ARWKAHLPTLRRTGDSFRLAIVPEDRWGNPTDRADQEIVLAPSL
                     AAAGLPERVRLRPGDGPLLIDGLSLQEPGDLAIGLETPAGAVLGRSNPLRVVDETPVL
                     HFWGDLHGQSNETVGTNTVQQYYAFARDRAFVDMVAHQGNDFQIDDALWAEINRLAAE
                     YDEPGRFVALPGYEWSGNTGMGGDHNVLYATEGRPIYRSSKVLLDEAQAKDPVCPRVD
                     DLFEALEGEDVVVLAHVGGRYADVAVAHDGRIEQAVEVHSCWGTFEWILHDAFALGYR
                     VGVVCHSDDHKGRPGAAYPGASLFGAIGGLTCYRMPSLDRPSLMAALRHRHHYGTTGT
                     RLYLDVEARFAGQAELLDSDPALGPAESEPVDAAVMGDIVRTGNDTVELAVDVLGSAP
                     IERVTVFNGAEPIAVHRPHAAADLGRRIRVIWEGAEYRGRSREVFWRGTCRLAGNRFE
                     RARAFNLFNQDKPLLLAADGSSVAFDNVTTGNFAGFDLWLEDAEAGELIFTSGVVEAK
                     VKIAEIGLDDTAFAAGGLGKGLRLFRLPDTGQTHKLAFTQEVELTPGEDNPLYVRVTQ
                     EDGHQAWSSPIYLIP"
     assembly_gap    1859..1958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1959..2951
                     /locus_tag="P5558_23240"
     CDS             <1959..2951
                     /locus_tag="P5558_23240"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY27299.1"
                     /translation="LISAGLGLALGTVGRDLFTNIERFTFSIRQLEGGISVVPMLLGL
                     LVFSEVLVQLRRELLGRGVHAEGAMKFDANSPPLSGRELRKHLPAILYGGSIGTIVGA
                     IPGLGATVGAFLAYSEAKRTAPADEPVGRGSLRGIAAAESGNSAGTGANLIPLVTLGI
                     PGNVEAALVLGAFMIHGLTPGPFLMQQQGPLLYAIFISLIAANLLLLLLGFGFLRLAR
                     YTLMINPRILLPIILVISTFGTYAATRQLFDVVIMLVFGLVGYAMRRAGFSPIPMLIA
                     FLLGPILEDGIRRSLLMSGGDLLIFFERPISLAFIALAMISLFLLFRRMAKARE"
BASE COUNT          433 a         1168 c          976 g          495 t
ORIGIN      
        1 gcccgctgga aggcgcacct gccgacgctg cggcggacgg gcgacagctt ccgcctcgcg
       61 atcgtgccgg aggaccgctg gggcaacccg accgaccggg ccgaccagga gatcgtgctc
      121 gcccccagcc tcgccgcggc gggcctgccc gagcgcgtcc gcctgcggcc gggcgacggc
      181 ccgcttttga tcgacggcct ctccctgcag gagccgggcg acctcgcgat cgggctcgag
      241 acgccggcgg gtgcggtgct cggccgctcc aacccgctgc gggtggtgga cgagacgccc
      301 gtcctgcatt tctggggcga cctgcacggc cagagcaacg agaccgtcgg caccaacacg
      361 gtccagcaat actacgcctt cgcccgcgac cgtgccttcg tcgacatggt cgcccatcag
      421 ggcaacgact tccagatcga cgacgcgctc tgggccgaga tcaaccggct cgccgccgaa
      481 tacgacgagc ccggccgctt cgtggcgctg ccgggctacg agtggtccgg caataccggc
      541 atgggcggcg accacaacgt gctctacgcc accgagggca gacccatcta ccgctcgtcc
      601 aaggtcctgc tcgacgaggc ccaggcgaag gacccggtct gcccccgggt cgacgacctc
      661 ttcgaggcgc tcgagggcga ggacgtggtg gtgctcgccc atgtgggcgg ccgctacgcc
      721 gatgtcgcgg tcgcccatga cggccggatc gagcaggccg tggaggtgca ctcctgctgg
      781 ggcaccttcg aatggatcct gcacgatgcc ttcgccctgg gctaccgcgt cggcgtcgtc
      841 tgccacagcg acgaccacaa gggccggccg ggtgccgcct atccgggcgc ctcgctcttc
      901 ggcgccatcg gcggcctcac ctgctaccgc atgcccagcc tcgaccggcc gagcctgatg
      961 gcggcgctcc gccaccgcca ccactacggc accaccggca cccggctcta tctcgacgtc
     1021 gaggcccgat tcgccggcca ggcggagctc ctcgacagcg accctgccct gggcccggcc
     1081 gagagcgagc cggtcgacgc cgcggtgatg ggcgacatcg tgcgaacagg caacgacacg
     1141 gtcgagctcg ccgtcgacgt gctcggctcg gcgccgatcg agcgggtgac ggtcttcaac
     1201 ggggccgagc ccatcgccgt ccaccgcccg cacgcagccg ccgatctcgg ccgccgcatc
     1261 cgggtgatct gggaaggggc ggagtatagg ggccgcagcc gcgaggtctt ctggcgcggc
     1321 acctgccggc tcgccggcaa ccgcttcgag cgtgcccggg ccttcaacct cttcaaccag
     1381 gacaagccgc tgctgctcgc cgccgacggc agcagcgtcg ccttcgacaa cgtcaccacc
     1441 ggcaatttcg ccggcttcga cctctggctc gaggatgccg aggccggcga gctgatcttc
     1501 accagcggcg tcgtcgaggc gaaggtgaag atcgccgaaa tcggcctcga cgacaccgcc
     1561 tttgcggccg gcgggctcgg caagggcctt cgcctcttcc gcctgccgga cacgggccag
     1621 acccacaagc tggccttcac ccaagaggtc gagctgacgc ccggcgagga caacccgctc
     1681 tacgtccgcg tcacccagga ggacggccac caggcctggt cgagcccgat ctacctcatc
     1741 ccctgagagc ctgagccgcg catcggggcg gagaccgcat gctcgacaac atcatcgccg
     1801 ccatcggcct ggtcttcacc ttccagagcc tcgtcttcgc ctgcttcggc gtcatcgtnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gatctcggcc gggctgggcc
     1981 tcgcgctcgg cacggtcggc cgcgacctct tcaccaatat cgagcgcttc accttcagca
     2041 tccgccagct cgaggggggc atcagcgtcg tgcccatgct gctcggcctc ctcgtcttct
     2101 ccgaggtgct ggtgcagctt cgccgcgagc ttctgggccg gggcgtgcat gccgaaggcg
     2161 ccatgaagtt cgacgccaac tccccgccgc tcagcggtcg cgagctgcgc aagcatctcc
     2221 cggccatcct ctatggcggc agcatcggca ccatcgtcgg cgccattccg ggcctggggg
     2281 ccacggtcgg cgcgtttctc gcctacagcg aggcgaagcg cacggcaccc gccgacgagc
     2341 cggtcggccg tggctcgctg cgcggcatcg ccgccgccga atccggcaac agcgccggca
     2401 ccggcgccaa cctcatcccg ctcgtcaccc tcggcatccc gggcaatgtc gaggcggcgc
     2461 tcgtgctcgg cgccttcatg atccacggcc tcacgcccgg ccccttcctc atgcagcagc
     2521 agggcccgct gctctacgcc atcttcatca gcctgatcgc ggcgaacctg ctgctcctgc
     2581 tgctcggctt cggcttcctg cgcctcgccc gctacacgct gatgatcaac ccgcgcatcc
     2641 ttctgccgat catcctcgtc atctcgacct tcggcacgta tgcggcgacc cgccagctct
     2701 tcgacgtcgt gatcatgctg gtcttcggcc tcgtcggcta cgccatgcgc cgggccggct
     2761 tctcgcccat ccccatgctc atcgccttcc tcctcggccc catcctcgag gacggcatcc
     2821 gccgctcgct cctcatgtcc ggcggcgatc ttcttatttt cttcgagcgc ccgatctcgc
     2881 tggccttcat tgccctggcg atgatttcgc tcttcctgct ctttcgccgg atggccaaag
     2941 cgcgggagtg agtcggggcg ggggccggcc gccggctggc tcgcgggaat gtgccgaaga
     3001 gctcctgccg attgcctgtc gcggcgcgtg tcccgacctg ccccccgtgt gccggctgcc
     3061 gcacggatga ctgcccaacg aaggaaagaa cacgccgatg cttcgcaagc gattgaacga
     3121 ggtggtgagc gacccgcggt tcgagcgggc gatcgtgatt ctgatcgtca tc
//
LOCUS       DAOTFI010000372         3164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_36230_length_3164_cov_7.399164, whole genome shotgun sequence.
ACCESSION   DAOTFI010000372 DAOTFI010000000
VERSION     DAOTFI010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36230_length_3164_cov_7.399164"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1114
                     /locus_tag="P5558_23245"
     CDS             137..1114
                     /locus_tag="P5558_23245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRY27300.1"
                     /translation="MSKITLGLALTSAIALSSPALAAYPEQPIQLIIAYGAGGATDLS
                     GRALATALGPKVPQPILPVNRTGAGGATGTASVAAAEPDGYTMVVARVGSHTVNPAMK
                     DTLPYTLDDFDFVAVYEINPVACAVAADSDIDSMETLAARVNENPGTVSYSSSGVGSL
                     LHIAGAMALRSFGVENPVEAAIHLPMAGGGEAATAVLAGTADFICTNSSALSSFVANG
                     QLRPLMVTTAEPVAGFDAPTSADLGKPELESLVGWSGIAGPAGLPDEVIETWRGWLEE
                     AVQDPGFQETMDRFGSIVDLKSPEDSVAFINEQYRIFRELVDELDMRVE"
     gene            1183..1677
                     /locus_tag="P5558_23250"
     CDS             1183..1677
                     /locus_tag="P5558_23250"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRY27301.1"
                     /translation="MSAPRTDFWIGVGVVALGSALALVAVPEGVVSPRNVRIAVLSPA
                     FWPNILAWVVALMGLVLALQSLRPAKTPDEPHEAAGAMPRHGLLRLLALGVGSIAFCA
                     FATKLGLVWASMIAFAVVLLLTGTSYRIAAVVVAILLPLALYAFFAHVAAVPIPQGIF
                     VRLP"
     gene            1674..3164
                     /locus_tag="P5558_23255"
     CDS             1674..3164
                     /locus_tag="P5558_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY27302.1"
                     /translation="MSFLDSAAAGFALVAHWESVFYLAMGVLVGVIAGAVPGVSATMA
                     VALALPFTFALEPIYGILLLLGVYKGGIFGGSIPAILIKTPGTPASSATTLDGYPMAE
                     RGEAGRALGMALYASCIADLISNLSLILLAGWLASFALSFGPPEFFTLILFSLTIIAG
                     VSGESLVKGLIAAASGLLLATVGLDLVYGTDRFSFGDPNLMGGLNFIAVLIGLFALPE
                     IIDFVFRPKEEHHQARQLGGRWATLADVRRCLRSIIRGSFIGVFLGAIPGIGGAPAAF
                     LSYAEAQRNSPNRDNFGKGEIEGVAAAEAGNNGVCGATLIPLLSLGIPGDVITAIIIG
                     AFMIHGLQPGPMLFVTHAHMVYALFMGLILSSFLLFAIGSVAIRAFRFVADVPKSLLA
                     PSVLVLCVFGTYAVNNTIFDVAVMFAMGWLGYLMRVLKIPAAPFLIGFILGPLLENSF
                     RQAMLMSGGSPEIFVRGPITWTFWALTALALYLIVARLRQSWRAET"
BASE COUNT          416 a         1142 c         1034 g          572 t
ORIGIN      
        1 ggcatcgagg cgctctatgc cgggctgaag cccccggcgc tgcggcgtta cggcgaaacc
       61 gacgatcgcc gggacgagct cctgtagccg gaagcgacaa tcggccagga atcaaataag
      121 agaaggaggc aagacgatgt cgaagatcac cctggggctg gcgctgacca gtgccatcgc
      181 cttgagctcg ccggcgctcg ccgcctatcc ggagcagccc atccagctca tcatcgccta
      241 tggggccggt ggtgcgacgg acctttccgg ccgggcgctg gcgacggcgc tggggcccaa
      301 ggtcccgcag ccgatcctgc cggtgaaccg gacgggtgcc ggcggggcca ccggcaccgc
      361 ctcggtcgcc gcggccgagc cggacggcta caccatggtg gtcgcccgcg tcggctcgca
      421 caccgtcaac ccggcgatga aggacacgtt gccctacacg ctcgacgatt tcgacttcgt
      481 cgcggtctac gagatcaacc cggtggcctg cgcggtcgcc gccgattccg acatcgacag
      541 catggagacg ctggccgccc gggtgaacga gaacccaggc accgtctcct acagctcgtc
      601 cggcgtcggc tcgctcctgc acatcgccgg ggccatggcg ctgcgctcgt tcggcgtcga
      661 gaacccggtg gaggcggcga tccatctgcc gatggcgggc ggcggcgagg cggcgacggc
      721 cgtgctggcg ggcacggcgg acttcatctg caccaactcc tcggcccttt cgagcttcgt
      781 cgccaatggt cagctccggc cgctcatggt caccacggcc gagccggtgg ccggcttcga
      841 tgcgccgacc tcggccgatc tcggcaagcc ggagctcgag agcctggtcg gctggtcggg
      901 catcgccggg cccgccgggc tgccggacga ggtcatcgag acctggcgcg gctggctcga
      961 ggaggcggtc caggacccgg gcttccagga gacgatggac cgcttcggct cgatcgtcga
     1021 cctgaagtcg cccgaggatt cggtcgcctt catcaacgag caataccgga tcttccgcga
     1081 gctcgtcgac gagctcgaca tgcgggtcga atagtccagg ccgggcggcg tcggcctggc
     1141 accggtgccg cccccaaccg cccagctcga acggacggtc ccttgagtgc tccgcgaacc
     1201 gatttctgga tcggcgtcgg cgtcgtcgcc ctcggctcgg cgctggcgct ggtggccgtg
     1261 cccgagggtg tcgtgtcgcc gcgcaacgtg cgcatcgccg tgctgtcgcc ggccttctgg
     1321 ccgaacatcc tggcctgggt cgtggcgctg atgggcctcg tcctcgccct gcagtcgctg
     1381 cgcccggcga agaccccgga cgaaccacac gaggcggcgg gtgcgatgcc ccgtcacggt
     1441 ctgctgcgcc tcctggcgct gggggtgggc tcgatcgcgt tctgcgcttt cgccaccaag
     1501 ctcggcctcg tctgggcgtc gatgatcgcc tttgccgtcg tcctcctgct caccggaacg
     1561 tcctaccgga tcgccgcagt cgtggtggcg atcctcctgc cgctggcact ctatgccttc
     1621 ttcgcccatg tcgccgcggt gccgatcccg cagggcatct tcgtgaggct gccttgagct
     1681 tcctcgacag cgccgccgcc ggcttcgccc tcgtcgccca ctgggagagc gtcttctatc
     1741 tagccatggg tgtcctcgtc ggcgtcatcg ccggggccgt ccccggcgtc tcggcgacca
     1801 tggcggtggc cctcgcgctg ccgttcacct tcgcgctcga gccgatctac ggcatccttt
     1861 tgctgctcgg cgtgtacaag ggcggcatct tcggcggctc gatcccggcg atcctgatca
     1921 agacgccggg cacgccggcc tcctcggcca cgacgctcga cggctatccc atggccgagc
     1981 ggggcgaggc ggggcgcgcg ctcggcatgg cgctctatgc ctcctgcatc gccgatctca
     2041 tctcgaacct gagcctgatc ctgctcgccg gctggctcgc ctcgttcgcc ttgagcttcg
     2101 gcccgccgga gttcttcacc ctcatcctct tctcgctcac catcatcgcc ggcgtctcgg
     2161 gcgagagcct ggtcaagggc ctgatcgccg ccgccagcgg gctcctgctc gccacggtcg
     2221 ggctcgacct cgtctatggc acggaccgct tcagcttcgg cgatccgaac ctcatgggcg
     2281 ggctgaactt catcgcggtg ctgatcggcc tcttcgccct gccggagatc atcgacttcg
     2341 tcttcaggcc caaagaggag caccaccagg cgcggcagct cggcggaagg tgggcgacgc
     2401 tcgccgacgt caggcgctgc ctcaggtcga tcatccgggg cagcttcatc ggcgtcttcc
     2461 tgggggccat tccgggcatc ggcggggcgc ccgccgcctt cctcagctat gccgaggcgc
     2521 agcgcaactc gcccaaccgg gacaatttcg gcaagggcga gatcgagggc gtggcggcgg
     2581 ccgaggccgg caacaacggc gtctgcgggg cgacgctgat cccgctgctc tcgctcggca
     2641 ttccgggcga tgtcatcacg gcgatcatca tcggcgcctt catgatccac gggctgcagc
     2701 ccgggccgat gctcttcgtc acccatgcgc acatggtcta cgccctcttc atgggcctca
     2761 tcctgagctc gttcctgctt ttcgccatcg gctcggtggc gatccgggcc tttcgcttcg
     2821 tcgccgacgt gccgaaatcg ctcctcgcac catcggtgct ggtgctctgc gtcttcggca
     2881 cctacgcggt caacaacacg atcttcgacg tcgccgtcat gttcgccatg ggctggctcg
     2941 gctacctcat gcgggtcctg aagattccgg ccgcaccctt cctcatcggc ttcatcctgg
     3001 ggccgctgct cgagaacagc tttcgccagg cgatgctgat gtccggcggc tcgcccgaga
     3061 tcttcgtgcg tggcccgatc acctggacct tctgggcgct gaccgccctt gccctctatc
     3121 tcatcgtcgc ccggctgagg cagtcctggc gggcggaaac ctag
//
LOCUS       DAOTFI010000373         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_36711_length_3132_cov_7.517387, whole genome shotgun sequence.
ACCESSION   DAOTFI010000373 DAOTFI010000000
VERSION     DAOTFI010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36711_length_3132_cov_7.517387"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="P5558_23260"
     CDS             <1..387
                     /locus_tag="P5558_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390232.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY27303.1"
                     /translation="GQDGHDRGQKVIASAFADLGFDVDIGPLFQTPAEAARQAVENDV
                     HVIGASSLAAGHLTLVPELRAELEKEGRGDIMIVVGGVVPPQDHAALKEAGARAIFPP
                     GTVIAEAAIDLLDKLAAELGHASAAE"
     gene            397..1392
                     /gene="meaB"
                     /locus_tag="P5558_23265"
     CDS             397..1392
                     /gene="meaB"
                     /locus_tag="P5558_23265"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRY27304.1"
                     /translation="MAASPELQDYVAGIEAGDRAMLARAITLVESRRPAHRAMARELL
                     AAILPAADPVHSKARRIGITGVPGVGKSTFIDAFGTMLTRRGHRVAVLAVDPSSSRSG
                     GSILGDKTRMARLAVDPGSFIRPSPSSGAMGGVARMTRETMLLCEAAGFDVVLVETVG
                     VGQSEIVVAGMVDFFLVLMLPGAGDELQGIKKGVLELADLIAVNKADGDNRLRAEQAA
                     VHYRRALSILEPVNPAWRPPVQLVSAIEETGLDDLWSAIERHRAILEAEGGLAARRGD
                     QQVRWLWSLLEARIEDRLRQELADELPGLEAAVGKGELAPGEAVDRLMARLFAGS"
     gene            complement(1389..1976)
                     /locus_tag="P5558_23270"
     CDS             complement(1389..1976)
                     /locus_tag="P5558_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27305.1"
                     /translation="MYRRPRFVASLPAKGALAGVLALVAGCAAGPRPSLEPGSSVLEA
                     RQLLVDTAAEGPVLVTWTGNPPLSEPSLLAAVERGVTGLRVETTTKGTAARRFDFRFD
                     PTGPAICGDAAFASTTPSRVQAAFCDGERAVSVASVEMTGNTDPERLIWQLTGRVVPD
                     DYEENYGFGWFGNMFSIGVGAGSGGGVGGGIGVGF"
     gene            2141..>3132
                     /locus_tag="P5558_23275"
     CDS             2141..>3132
                     /locus_tag="P5558_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27306.1"
                     /translation="MFDTPAVRIGVVGTGFVARHFCLELQHRKPYRLARVLTRRPLAG
                     TTEFPFQDALTNSLVELIDHSDIIFECTGDVLHATLTIEAALAAGKPVLTLNPEFHVT
                     TGSWFADQGYLTEAEGDQPGCQASLWEEAVAMGFEPLVLGNMKGFLNRFPVPEEMAFW
                     AEKQGISTTMVTSFTDGTKLQVEQALVGNFFGAGIAQDELIGPETDDLKEASTILGDA
                     ARRLGRPIADYVLSRKLPHGVFIVAAHEAEQQDALRYLKMGEGPYYTIIRNNIFVHLE
                     VFRTLDRMVRRREPLLHNTTHPTIGVAAVAKRDLAPGEAIERGCGSFDMRGICVR"
BASE COUNT          458 a         1152 c         1081 g          441 t
ORIGIN      
        1 ggccaggacg gccacgaccg cggccagaag gtgatcgcct cggccttcgc cgatctcggc
       61 ttcgacgtcg atatcggccc gctcttccag accccggccg aggcggcccg ccaggcagtc
      121 gagaacgacg tccacgtgat cggcgccagc tccctcgccg ccggccacct gaccctcgtg
      181 ccggagctgc gggccgagct cgagaaggag ggccggggcg acatcatgat cgtcgtcggc
      241 ggggtcgtgc cgccgcagga ccacgcggcc ctgaaggagg ccggggccag ggccatcttc
      301 ccgcccggca cggtgatcgc cgaggccgcc atcgacctgc tcgacaagct cgctgccgag
      361 ctcggccacg cctcggcggc cgagtaaccg cttcccttgg ctgcttcgcc cgagctccag
      421 gactacgtcg ccggcatcga ggccggcgac cgggccatgc tggcgcgcgc catcaccctg
      481 gtcgagagcc gccggccggc gcaccgggcg atggccaggg agctcctcgc cgccatcctg
      541 cccgccgccg acccggtcca ctcgaaagcg cggcgcatcg gcatcaccgg tgttcccggc
      601 gtcggcaaga gcaccttcat cgacgccttc ggcaccatgc tcactcgaag gggccaccgg
      661 gtggcggtgc tcgccgtcga cccctcctcg tcacgcagcg gcgggtcgat cctcggcgac
      721 aagacgcgca tggcccggct cgccgtcgat ccggggagct tcatccgccc ctcgccctcc
      781 tccggggcca tgggcggggt ggcgcggatg acccgcgaga ccatgctgct ctgcgaggcc
      841 gccggcttcg acgtggtgct ggtcgagacg gtgggggtcg gccagtccga gatcgtcgtc
      901 gccgggatgg tcgatttctt cctcgtcctg atgctgcccg gcgccggcga cgagctgcag
      961 ggcatcaaga agggcgtgct cgagctcgcc gacctcatcg ccgtcaacaa ggccgacggc
     1021 gacaaccggc tgcgggccga gcaggcggcc gtccactatc gccgagcgct ctcgatcctc
     1081 gagcccgtga accctgcctg gcgaccgccg gtgcagctcg tcagcgccat cgaggagacc
     1141 gggctcgacg acctctggag cgccatcgag cggcatcggg ccatcctcga agccgaaggc
     1201 ggcctcgccg ccaggcgcgg cgaccagcag gtgcgctggc tctggtcgct gctggaggca
     1261 cggatcgagg accggctgcg ccaggagctg gcggacgagc tgccggggct cgaggcggcg
     1321 gtcggcaagg gcgagctcgc ccccggcgaa gccgtcgacc ggctcatggc gcggcttttc
     1381 gcgggctcct agaacccgac cccgataccg ccgcccacgc cgccgccact gccggcaccg
     1441 acgccgatgc tgaacatgtt gccgaaccag ccgaagccgt agttctcctc gtagtcgtcg
     1501 ggcacgaccc gcccggtgag ctgccagatc agccgctccg ggtcggtgtt cccggtcatc
     1561 tcgacgctgg cgaccgagac cgcgcgctcg ccgtcgcaga aggcggcctg gacccggctc
     1621 ggcgtggtcg aggcgaaggc ggcatcgccg cagatggcgg ggcccgtcgg gtcgaagcgg
     1681 aagtcgaagc gccgcgccgc cgtgcccttg gtcgtggttt ccacccggag cccggtgacg
     1741 ccgcgctcga ccgcggcgag gagcgacggc tcgctcaagg gcgggttgcc cgtccaggtg
     1801 acgagcaccg gcccctcggc cgcggtgtcg accaagagct ggcgcgcctc gagcacgctg
     1861 gagccgggct cgagcgacgg ccgcggcccc gcagcgcacc cggcgaccag ggccagcacg
     1921 ccggcgaggg cacccttggc ggggagcgaa gcgacaaagc gcggcctgcg gtacatcggg
     1981 gattcacctt cgacggtcga acgacggggc acgttaacac ctcgctaggc ggttgccgct
     2041 atcctgatgc gtggaagtgg cgtcggcaat ggtgctcgca cagggtgcgg gggcttcggc
     2101 cggcgttttc cgtctgtcca tgacaagaag gtccgcccgc atgttcgaca ctcccgccgt
     2161 gcgcatcggc gtcgtcggca ctggcttcgt cgcccgccat ttctgcctcg agctgcagca
     2221 caggaagccc taccggctgg cccgggtgct gacccgccgc ccgctcgccg gcacgaccga
     2281 gttccccttc caggatgcgc tgaccaactc gctcgtggag ctgatcgacc attcggacat
     2341 catcttcgag tgcaccggcg acgtgctgca tgcgacgctg acgatcgagg cggcgctcgc
     2401 cgccggcaag cccgtgctca ccctcaatcc cgagttccac gtcaccacgg gctcctggtt
     2461 cgccgaccag ggctatctga ccgaggccga gggcgaccag ccgggctgcc aggcctcgct
     2521 ctgggaggag gcggtcgcca tgggcttcga gcccctcgtc ctcggcaaca tgaagggctt
     2581 cctcaaccgc ttcccggtgc cggaggagat ggccttctgg gccgagaagc agggcatcag
     2641 cacgaccatg gtgacctcct tcaccgacgg caccaagctg caggtcgagc aggcgctggt
     2701 cggcaatttc ttcggcgccg gcatcgcgca ggacgagctg atcgggcccg agaccgacga
     2761 cctgaaggag gcctcgacca ttctcggcga cgcggcgcgc cggctcggcc ggccgatcgc
     2821 cgactacgtc ctgagccgca agctgccgca cggcgtcttc atcgtggcgg cgcacgaggc
     2881 cgagcagcag gacgcccttc gctacctgaa gatgggcgag ggcccctact acacgatcat
     2941 ccgcaacaac atcttcgtcc atctcgaggt gttccgcacg ctcgaccgga tggtgcgccg
     3001 gcgcgagccc ctgctgcaca acacgaccca tccgacgatc ggcgtggccg ccgtcgccaa
     3061 gcgcgacctc gccccgggcg aggcgatcga gcgcggctgc ggcagcttcg acatgcgcgg
     3121 catctgcgtg cg
//
LOCUS       DAOTFI010000374         3125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_36805_length_3125_cov_7.482410, whole genome shotgun sequence.
ACCESSION   DAOTFI010000374 DAOTFI010000000
VERSION     DAOTFI010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36805_length_3125_cov_7.482410"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1599
                     /locus_tag="P5558_23280"
     CDS             253..1599
                     /locus_tag="P5558_23280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27307.1"
                     /translation="MTNKGAFLGSMILGASVLLPAPVAAQTASDLRALIEAQAEQLAA
                     QARQLEEQTRQLEAMRQRLDHLEAAEGTAEVAQVPFEGEPTEPPPAFEVVAPETPPAQ
                     TMPERVITSGQPEIEVAISGHINRMINVADDGDSTKTYFVDNGNSVSRVRLVGTGRID
                     ETLSIGTAIELGIAPNPSFRVSQDNEDAGDFTDQRIVQAYVDSTRFGRLALGKGPMAS
                     DGAGLQDISGTSVIQYSAIADTAGGLQFVSDGDLTGISISNVFSNLNGSRRDRIRYDT
                     PSLAGFSLATSFASNQRYDGAVYWAGRNDTWEATAAAAVQQPNIDDVDVRLVSSGSML
                     HRPTGLNATFNAGRDIRDGRDYSTLYGKLGVITELVSLGPTAFSVDYDWADDRPINSA
                     AAITADGSSAGFAVVQDWTRFGAELYFGLRWHKAELRGGDDVDDILVSSFGTRVRF"
     gene            1607..1801
                     /locus_tag="P5558_23285"
     CDS             1607..1801
                     /locus_tag="P5558_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27308.1"
                     /translation="MSLVLVAGILLLAGCGMEPFDPPRPDELQPGPGILTGESGELVI
                     YRRGQEEGRPDGAPTGTPDP"
     gene            1897..>3125
                     /locus_tag="P5558_23290"
     CDS             1897..>3125
                     /locus_tag="P5558_23290"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY27309.1"
                     /translation="MTERKEHELILQEQWRRRLLRGEIDRRQFLSRTVTAGLGMAGVG
                     VAANFGMGARSALAARPLTPTFYQWIPDLHPAIPEVNAGFPDLNFQIAPVEGFGIERF
                     VAEAKDQKSTWDVYVGMTPFVEMTAMIEADVIEPWDNYIPKEVIDDILPAIREECSID
                     GKLYSWPFLLDVIGMSWHAGITDQAGLGGMPPEDWDAYLAAAQTIVDSGAAPYGATFD
                     AHGWRSLAPFTHSMSPDVYTSEGLFDFTSDAAVEALILMKNIMALSNPDILLAGTTDG
                     GVNGTPDELAFAAQRVGLYTKYFNAPLRMAQYWDDPAMLSMGGLPKFKGGAGSTVFWT
                     TGSALFKHGENKEKAAEYIQALTYNPQIWQDSIAGTATAHPGQLPPYASIYAGWNADR
                     PDWLPPFVDLVRGQLDRA"
BASE COUNT          508 a         1133 c         1014 g          470 t
ORIGIN      
        1 agtcgttaac gggtgccagc ctaggagcct gtccgagaaa tggccaccgc ctgcacgcgc
       61 ccttcgggtc gctctctggc aggcggtggc catttctcgg acaggctcct cgcagcgtca
      121 gtggccggcg gcgagccggt gcacgggcca caggtggccg gcattgcgtc ggctcgccgc
      181 tttatcccct tgcaactcct cgccgggttc agcagcctcg ccgaaaatgg cgtggggtcg
      241 cgggaggcca tcatgacgaa caagggggcg tttctcggct cgatgatcct cggcgcgtcc
      301 gtccttctgc cggcacccgt ggccgcgcag accgccagcg atctccgggc gctgatcgag
      361 gcccaggccg agcagctggc ggcccaggcc cggcagctcg aggagcagac ccgccagctc
      421 gaggcgatgc ggcagcgtct cgatcatctc gaggcggccg aaggcacggc cgaggtggcc
      481 caggtgccct tcgagggcga gccgaccgag ccgccgccgg ccttcgaggt ggtggcgccc
      541 gagaccccac cggcccagac gatgcccgag cgcgtcatca cctccggaca accggagatc
      601 gaggtcgcca tctccggcca catcaaccgc atgatcaacg tcgccgacga tggtgacagc
      661 accaagacct acttcgtcga caacggcaac tcggtctcga gggtccgcct ggtcggcacc
      721 ggccggatcg acgagacgct gtcgatcggc acggcgatcg agctgggcat cgcccccaac
      781 ccctccttcc gcgtctcgca ggacaacgag gacgccggcg atttcaccga ccagcgcatc
      841 gtccaggcct atgtcgacag cacccgcttc ggccgcctgg cgctcggcaa gggtcccatg
      901 gcatccgacg gcgccgggct gcaggacatc tccggcacct cggtcatcca gtactcggcc
      961 atcgccgaca cggcgggcgg cctgcagttc gtcagcgacg gcgacctcac cggcatcagc
     1021 atctccaatg tcttcagcaa cctgaacggg tcgcgccgcg atcgcatccg ctacgacacg
     1081 cccagcctcg ccggcttctc gctggccacc tcgttcgcca gcaaccagcg ctatgacggt
     1141 gcggtctact gggccggccg gaacgacacc tgggaggcga cggcggcggc ggccgtgcag
     1201 cagcccaaca tcgacgatgt cgacgtgcgg ctggtcagct ccggctcgat gctgcatcgg
     1261 ccgaccggcc tcaacgcgac gttcaatgcg ggtcgcgaca tccgcgacgg gcgcgactac
     1321 tcgacgctct acggcaagct cggggtgatc accgagctgg tgtcccttgg cccgaccgcc
     1381 ttctcggtcg actacgactg ggcggacgac cggccgatca actcggccgc ggccatcacc
     1441 gcggacggca gctcggccgg cttcgccgtc gtccaggact ggacccgctt cggcgccgag
     1501 ctctatttcg ggctgcgctg gcacaaggcc gagctgcgcg gtggcgacga tgtggacgac
     1561 atcctcgtca gcagcttcgg cacgcgcgtc cggttctgat gtggctatga gcttggtcct
     1621 cgtggccggc atcctgctgc tcgccggctg cggcatggag ccgttcgacc cgccgcgccc
     1681 cgacgagctg cagccggggc cgggcatcct gaccggcgag agcggcgagc tcgtcatcta
     1741 ccggcggggg caggaggaag ggcggccgga cggagccccg accggcaccc cggatccctg
     1801 agcagactcg atgttgccaa ggccacggcc ccgattataa ctgctcgaag gccaagaacg
     1861 acaaacgcca agcaagcttc ggagggacgt tcgaccatga ctgaacggaa agagcacgag
     1921 ctgatccttc aggagcaatg gcgcaggcgc ctgctgcgcg gcgagatcga ccgccggcag
     1981 ttcctgtcgc gcacggtgac cgccgggctc ggcatggccg gtgtcggcgt cgccgccaat
     2041 ttcggcatgg gtgcccggtc ggcgctcgcc gcccgccccc tgacgccgac cttctaccag
     2101 tggatcccgg acctgcaccc ggccatcccg gaggtgaacg ccggcttccc cgacctcaac
     2161 ttccagatcg ccccggtcga gggcttcggc atcgagcgct tcgtcgccga ggccaaggat
     2221 cagaagagca cctgggacgt ctatgtcggc atgacgccgt tcgtcgagat gacggcgatg
     2281 atcgaggccg acgtcatcga gccctgggac aactatatcc cgaaggaggt gatcgacgac
     2341 atcctgccgg cgatccgcga ggagtgctcg atcgacggca agctctacag ctggccgttc
     2401 ctgctcgacg tgatcggcat gagctggcac gccggcatca ccgaccaggc cgggctcggc
     2461 ggcatgccgc cggaggactg ggacgcctat ctcgcggcgg cgcagaccat cgtcgactcg
     2521 ggcgccgccc cctatggcgc caccttcgac gcgcatggct ggcgctcgct cgcccccttc
     2581 acccattcga tgtcgcccga cgtctacacg tccgagggcc tcttcgactt caccagcgac
     2641 gccgccgtcg aggcgctgat cctgatgaag aacatcatgg cgctttccaa tcccgacatc
     2701 ctgctcgccg ggacgaccga tggcggcgtc aacggcacgc cggacgagct cgccttcgcc
     2761 gcccagcgcg tcggcctcta caccaagtac ttcaatgcgc ccctgcgcat ggcgcagtac
     2821 tgggacgatc cggccatgct gagcatgggc ggcctgccga agttcaaggg cggcgccggc
     2881 tccaccgtct tctggaccac gggctcggcc ctcttcaagc acggcgagaa caaggagaag
     2941 gcggccgagt acatccaggc gctgacctac aacccgcaga tctggcagga ttccatcgcc
     3001 ggcacggcga ccgcgcatcc gggtcagctc ccgccctacg cctcgatcta tgccggctgg
     3061 aacgccgacc ggccggactg gctgccgccc ttcgtcgacc tcgtccgcgg ccagctcgac
     3121 cgggc
//
LOCUS       DAOTFI010000375         3108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_37083_length_3108_cov_6.825090, whole genome shotgun sequence.
ACCESSION   DAOTFI010000375 DAOTFI010000000
VERSION     DAOTFI010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37083_length_3108_cov_6.825090"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="P5558_23295"
     CDS             <1..251
                     /locus_tag="P5558_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27310.1"
                     /translation="EAIERGCGSFDMRGICVRLAEHERHLPIGLAEGVRIVRPIQKDQ
                     VISLDDVELPASRALDIWQKIEERVLGRPPGRKDARAA"
     gene            374..577
                     /locus_tag="P5558_23300"
     CDS             374..577
                     /locus_tag="P5558_23300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27311.1"
                     /translation="MRIFWTMALTLGTLVACSSEPETVTVYCYETLADSACYFEPDPG
                     HGNRLLAVMEVKLTPEVRLRAGF"
     gene            631..1494
                     /locus_tag="P5558_23305"
     CDS             631..1494
                     /locus_tag="P5558_23305"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRY27312.1"
                     /translation="MRERLEPSLNRRLFLAGATSLAVTPRMGAAEAGIEPREFGVAAL
                     GRPLPYIAYATAGAGPGAPIVYLLHGHGGSEWSWVRAGAALETAEALVATGELPPLHL
                     VLPGVGNSWYVDSDRHGPVATTLLDGLMPALEAELAVDPDRRGLIGLSMGGFGTLHLG
                     LREPERFRFLGALSPAVFEPDAAFPDIQLGLFAGAFGEPFDPSLFAAADPFNRIPLAA
                     AAPRPPDFYLATGADDYFGLEIGTFAFYRALEEAGVAAQVRILDGRHDWAFWRDQLPA
                     TLRRFAAAIAA"
     gene            complement(1517..2227)
                     /locus_tag="P5558_23310"
     CDS             complement(1517..2227)
                     /locus_tag="P5558_23310"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRY27313.1"
                     /translation="MPYFLFGLLVLGLLLLGSQWFTRANPRDVAQAARIATVAIGGLV
                     GIGLLLAGRFGLAFLIMAGLWTAIRAIRRHRGGADPMQSGPAGDPVEIRTDLLQMRLD
                     RATGEVDGAVRQGRFAGSELGDLGLGELLELLAEAGRTDPQSSSLLEAYLDRRFPDWR
                     EAAQEGPAAAGGAGMDEQQALDILGLQKGAGPDEIKEAHRRLMTKVHPDSGGSNFLAA
                     QINRAKDLLLGAARSHGD"
     gene            complement(2241..2942)
                     /locus_tag="P5558_23315"
     CDS             complement(2241..2942)
                     /locus_tag="P5558_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY27314.1"
                     /translation="MANRRDITDRAPDAVSAFVEEMRRAPAPADAVGRGRLIFALDAT
                     ASRRPTWDQACSVQGDMFLEADRLGGLDVQLAFYRGYDECKASRWVGQAGELIKLMVR
                     VGCRAGRTQIHRILRHAINETEKRRVHALIFIGDSCEESVDTLGDLAGRLGLLGVKAF
                     VFHEGNDREAAYAFQEIARLTGGAACRFDASAPAELRALLRAVAAYAAGGRKALVDLS
                     KREGGPVALLERQIR"
BASE COUNT          423 a         1168 c         1059 g          458 t
ORIGIN      
        1 gcgaggcgat cgagcgcggc tgcggcagct tcgacatgcg cggcatctgc gtgcgcctcg
       61 ccgagcacga gcggcatctg ccgatcggcc tcgccgaggg ggtccggatc gtccgcccga
      121 tacagaagga tcaggtgatc agcctcgacg acgtcgagct gccggcctcg cgcgccctcg
      181 acatctggca gaagatcgag gagcgcgtgc tcggcaggcc gcccggccgg aaggacgcgc
      241 gggccgcctg agccggccgt tccaccaggg ttgacgcgcg gtcgttcccg ggtgcgtcat
      301 cgctaacgac tgttaaccgc cgccgccccg catcctctac cctccttcgg gtcacgagcg
      361 atgcgggacg gcgatgcgga tcttctggac gatggcgctg acgctcggca cgctcgtcgc
      421 ctgcagcagc gagcccgaga ccgtcacggt ctactgctac gagacgctcg ccgattccgc
      481 ctgctatttc gagcccgacc ccggccacgg caaccgcctg ctggccgtca tggaggtgaa
      541 gctgaccccg gaggtcaggc tccgcgccgg cttctgacgc ctcgtttggg gagttgcgtc
      601 ggacctcggg atcggcgaag gaacgcgccc atgcgcgagc gtctcgaacc ctcactcaat
      661 cgccggctct tcctcgccgg tgccacctct ttggcggtta cgccgcgcat gggcgcggcc
      721 gaggccggca tcgagccgcg ggaattcggc gtcgccgcgc tcggccggcc gctgccctac
      781 atcgcctacg ccacggcggg tgccgggccc ggcgcaccca tcgtctacct gctgcacggc
      841 catggcggca gcgagtggtc ctgggtgcgg gccggtgccg cgctggagac ggcggaagcg
      901 ctggtcgcca cgggcgagct gccgcccttg catctcgtcc tgcccggggt cggcaatagc
      961 tggtatgtcg acagcgaccg ccacgggccc gtcgccacca cgctgctcga cgggctgatg
     1021 ccggcgctcg aagccgagct cgccgtcgat cccgacaggc gcggcctgat cggcctctcg
     1081 atgggcggct tcggcaccct gcatctgggc ctgcgcgagc cggagcgctt tcgtttcctc
     1141 ggcgccctga gccccgccgt cttcgagccc gacgccgcct ttccggacat ccagctcggc
     1201 cttttcgccg gggccttcgg cgagcccttc gacccctcgc tcttcgccgc cgccgatccc
     1261 ttcaaccgca ttccgctggc cgccgccgct ccccgtccgc ccgacttcta cctcgccacc
     1321 ggcgccgacg actatttcgg cctggagatc ggcaccttcg ccttctatcg cgccctcgag
     1381 gaagccggtg tcgcggccca ggtgcgcatc ctcgacggcc ggcacgactg ggctttctgg
     1441 cgcgaccagc tccccgcgac gctgcgtcgc ttcgcggccg cgatcgccgc ctgagcccgc
     1501 cacaggggat cggttctcag tcgccgtggc tccgggccgc gccgagcagc aggtccttcg
     1561 cccggttgat ctgggccgcc aaaaaattgc tgccgccact gtcggggtgc accttggtca
     1621 tcagccgccg atgcgcctcc ttgatctcgt ccggcccggc acccttctgc aggccgagaa
     1681 tgtcgagcgc ctgctgctcg tccatgcccg caccaccggc cgcggccggc ccttcctgcg
     1741 cggcctcgcg ccagtcgggg aagcggcggt cgagataggc ctcgagcagg ctgctgctct
     1801 gcggatcggt gcggcccgcc tcggccaaaa gctcgagcag ctcacccagc ccgagatcgc
     1861 ccagctcgct tccggcgaag cgaccctgcc gcacggcccc gtcgacctcg ccggtcgccc
     1921 ggtcgagccg catctgcagg aggtcggtgc ggatctccac cggatccccg gccggcccgc
     1981 tttgcatcgg gtcggcgccg ccgcgatggc ggcggatcgc ccggatcgcc gtccacaggc
     2041 cggccatgat gagaaaggcg aggccgaagc ggccggccag cagcaggccg atgccgacga
     2101 ggccgccgat cgccaccgtg gcgatccggg cggcctgcgc cacgtcccgc ggattggcgc
     2161 gcgtgaacca ctggctgccc agcaggagca ggccaaggac cagaagcccg aaaaggaaat
     2221 agggcatgcg gcctgtgagc ctagcgaatc tggcgctcga gcagggcgac cgggccgccc
     2281 tcccgcttcg agagatcgac gagcgccttg cgcccgcccg ccgcataggc cgcgaccgcg
     2341 cggagcaggg cgcgaagctc ggccggtgcc gaggcatcga agcgacaggc cgcaccgccg
     2401 gtcagccgcg cgatctcctg gaaggcatag gccgcctccc ggtcgttgcc ctcgtggaag
     2461 acgaaggcct tcacacccag gagcccgagc cggccggcga gatcgcccag cgtgtcgacc
     2521 gattcctcgc aagaatcacc gatgaagatc aaggcgtgga cccgccgctt ctccgtctcg
     2581 ttgatggcgt ggcgcaggat ccggtggatc tgcgtgcggc cggcgcggca gccgacgcgg
     2641 accatgagct tgatcagctc gccggcctgg ccgacccagc ggctcgcctt gcactcgtcg
     2701 tagccgcgat agaaggcgag ctgcacgtcg aggccgccga gccggtccgc ctcgaggaac
     2761 atgtcgccct gcaccgagca ggcctggtcc caggtcgggc gcctggaggc cgtggcatcg
     2821 agcgcgaaga tcagccgccc gcggccgacc gcgtcggccg gcgccggggc gcgccgcatc
     2881 tcctcgacga aggcgctgac cgcgtcgggc gcgcggtcgg tgatgtccct gcgattggcc
     2941 atggctcata tgttggccgg ccggcccggg attccagtgg ttcgggccag tggcccgggc
     3001 ccgatgccgc cgctcagtac gtggcccggc cgcccgagag gtcgaaaacc gcaccggtcg
     3061 tgaacgagtt ctcggccgag acgatccagg cgaccatcgc cgccgcct
//
LOCUS       DAOTFI010000376         3104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_37140_length_3104_cov_9.330928, whole genome shotgun sequence.
ACCESSION   DAOTFI010000376 DAOTFI010000000
VERSION     DAOTFI010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37140_length_3104_cov_9.330928"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="P5558_23320"
     CDS             <1..1673
                     /locus_tag="P5558_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRY27315.1"
                     /translation="FMPWNGYNFEDSILISERIVREDIFTSIHIDEFEVMARDTKLGP
                     ESITRDIPNVSEEALRNLDEAGIVYIGAEVKAGDIMVGKVTPKGETPMTPEEKLLRAI
                     FGEKAADVRDTSLKVPPGVQGTVVEVRVFNRRGVDKDERAIAIERAEIERLAKDRDDE
                     RSILERNIFGRLRELLDEQKVTAGPKGVKAGTIIDPALLDATPRRQWWDLAIDDVERM
                     TELQALKRQLDDGIKRLTERFENKVEKLQRGDEMPPGVLKMVKVFVAVKRKLQPGDKM
                     AGRHGNKGVISKIAPLEDMPCLEDGTPVDIVLNPLGVPSRMNVGQILETHLGWACANL
                     GRQIGQIVDRLDEPGMREALLGKVDTIYGNGVSAEVGAYEPHELAELGQNLSRGVPIA
                     TPVFDGAHEPDIVALLEEAGCHASGQSRLIDGRTGEPFDRPVTVGYIYMLKLGHLVDD
                     KIHARSVGPYSLVTQQPLGGKAQFGGQRFGEMECWALQAYGAAYSLQEMLTVKSDDVS
                     GRTKIYEAIVRGEDTFEAGIPESFNVLVKELQALGLNVELQQETVGED"
     gene            1763..>3104
                     /locus_tag="P5558_23325"
     CDS             1763..>3104
                     /locus_tag="P5558_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRY27316.1"
                     /translation="MNDLLYLFGQPATGQKFDQIQIAIASPDQIREWSYGEVRKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRMKYKGIVCEKCGVEVIQSKVRRERM
                     GHIELAAPVAHIWFMKSVPSRIGILLDMTLRDLERVLYFENWVVVEPGLTSLKQHELL
                     NEEQVMRHRDEFGDDFQVGIGAEAVRDMLKALDLPREREQMRADLLETTSEAKRKKLV
                     KRLKLVETFLESGNKPEWMVLEVVPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLIELRAPDIIVRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLSDMLKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFVYAKLELYGKAQ
                     TIKAAKRMVEKERPEVWDILEQVIREHPVMLNRAPTLHRLGIQAFEPMLIEGKAI"
BASE COUNT          579 a         1033 c         1035 g          457 t
ORIGIN      
        1 ccttcatgcc gtggaacggc tacaacttcg aggactcgat cctgatctcc gagcgcatcg
       61 tgcgcgagga catcttcacc tcgatccata tcgacgagtt cgaggtgatg gcccgcgaca
      121 ccaagctcgg gccggagagc atcacccgcg acatcccgaa tgtctcggag gaggcgctca
      181 ggaacctcga cgaggcgggc atcgtctata tcggtgcgga ggtgaaggcc ggcgacatca
      241 tggtcggcaa ggtcacgccc aagggcgaga cgccgatgac gccggaagag aagctgctgc
      301 gcgccatctt cggcgagaag gcggcggacg tgcgcgacac ctcgctcaag gtgccgccgg
      361 gcgtgcaggg caccgtggtc gaggtccgtg tcttcaaccg tcgcggcgtc gacaaggacg
      421 agcgtgccat cgccatcgag cgggccgaga tcgagcgtct cgccaaggac cgcgacgacg
      481 agcgcagcat cctcgagcgc aacatcttcg gccgcctgcg cgagctgctc gacgagcaga
      541 aggtcaccgc cggccccaag ggggtgaagg ccgggaccat catcgacccg gcgctgctcg
      601 acgccacgcc ccgccgccag tggtgggacc tcgcgatcga cgatgtcgag cggatgaccg
      661 agctgcaggc cctcaagcgg cagctcgacg acggcatcaa gcggctgacc gagcgcttcg
      721 agaacaaggt cgagaagctg cagcgcggcg acgagatgcc gcccggcgtg ctcaagatgg
      781 tcaaggtgtt cgtcgcggtg aagcgcaagc tgcagccggg cgacaagatg gccggccggc
      841 acggcaacaa gggcgtgatc tcgaagatcg ccccgctcga ggacatgccg tgcctcgagg
      901 acggcacccc cgtcgacatc gtgctcaatc ccttgggcgt gccgtcgcgg atgaatgtcg
      961 gccagatcct ggagacccat ctgggctggg cctgcgccaa tctcggacgg cagatcggcc
     1021 agatcgtcga tcggctcgac gagcccggga tgcgcgaggc cctgctcggc aaggtcgaca
     1081 ccatctacgg caatggcgtc agcgccgagg tcggtgccta cgagccgcac gagctggccg
     1141 agctcggcca gaacctgagc cgcggcgtgc cgatcgccac gccggtcttc gacggtgccc
     1201 acgagcccga catcgtggcc ctgctcgagg aggccggctg tcacgcctcg ggccagtcgc
     1261 ggctgatcga cggccggacg ggcgagccgt tcgatcgccc ggtgacggtc ggctacatct
     1321 acatgctgaa gctcggccat ctcgtcgacg acaagatcca cgcccgctcc gtcggcccct
     1381 acagcctggt cacccagcag ccgctgggcg gcaaggcgca gttcggcggc cagcggttcg
     1441 gcgagatgga atgctgggcg ctgcaggcct acggcgcggc ctacagcctc caggagatgc
     1501 tcaccgtcaa gtccgatgac gtgtccgggc gcaccaagat ctacgaggcc atcgtgcgcg
     1561 gcgaggacac gttcgaggcg ggcatccccg agagcttcaa cgtgctcgtg aaggagctgc
     1621 aggccctcgg cctcaatgtc gagctgcagc aggaaacggt gggcgaggac tgagccaggt
     1681 cccgtctatt cctcagcccg ggcggtccgg ccccgaccgc ccggcgctcc ctcaagagcg
     1741 gcgctagaga gcaggtacca ccatgaacga tctcctctac ctcttcggcc agcccgcgac
     1801 cggtcagaag ttcgaccaga tccagatcgc gatcgccagc ccggaccaga tccgcgagtg
     1861 gtcgtatggc gaggtcagga agcccgagac catcaactac cggaccttca agccggagcg
     1921 ggatggcctc ttctgcgcca agatcttcgg cccgatcaag gactacgagt gcttgtgcgg
     1981 caagtacaag cgcatgaagt acaagggcat cgtctgcgag aagtgcggcg tcgaggtcat
     2041 ccagtcgaag gtccgccgcg agcgcatggg ccatatcgag ctggccgccc cggtcgccca
     2101 tatctggttc atgaagtcgg tgccgtcgcg catcggcatc ctgctcgaca tgacgctgcg
     2161 tgacctcgag cgcgtcctct atttcgagaa ctgggtggtg gtcgagccgg gcctcacctc
     2221 gctgaagcag cacgagctcc tcaacgagga gcaggtgatg cgtcaccggg acgagttcgg
     2281 cgacgacttc caggtcggca tcggcgccga ggccgtgcgc gacatgctga aggcgctgga
     2341 cctgccgcgc gagcgcgagc agatgcgcgc cgacctgctc gagaccacgt cggaggccaa
     2401 gcgcaagaag ctggtcaagc ggctgaagct ggtcgagacc ttcctcgagt ccggcaacaa
     2461 gcccgaatgg atggtgctcg aggtcgtgcc ggtcatcccg ccggagctgc gcccgctcgt
     2521 gccgctcgac ggcggccgct tcgccacatc cgacctcaac gatctctacc ggcgcgtcat
     2581 caatcgcaac aaccgcctga agcggctgat cgagctgcgt gcccccgaca tcatcgtccg
     2641 caacgagaag cggatgctgc aggaggcggt ggacgcactc ttcgacaacg gccgccgcgg
     2701 ccgggtcatc accggcgcca acaagcggcc cttgaagtcg ctctccgaca tgctcaaggg
     2761 caagcagggc cgcttccgcc agaatctgct cggcaagcgc gtcgactatt cgggccgttc
     2821 ggtgatcgtc gtcgggccgg agctcaagct gcaccagtgc ggcctgccga agaagatggc
     2881 gctcgagctc ttcaagccct tcgtctatgc caagctcgag ctctacggca aggcgcagac
     2941 catcaaggcc gccaagcgga tggtcgagaa ggagcggccc gaggtctggg acatcctcga
     3001 gcaggtgatc cgcgagcacc cggtcatgct gaaccgggcg ccgacgctgc accgcctcgg
     3061 catccaggcg ttcgagccga tgctgatcga gggcaaggcg atcc
//
LOCUS       DAOTFI010000377         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_37549_length_3079_cov_5.825728, whole genome shotgun sequence.
ACCESSION   DAOTFI010000377 DAOTFI010000000
VERSION     DAOTFI010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37549_length_3079_cov_5.825728"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="P5558_23330"
     CDS             complement(<1..472)
                     /locus_tag="P5558_23330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27317.1"
                     /translation="MRRCYQAILIALAGSLLLPGLAGATSACDGLIGTWWWPDGSRVT
                     LRDDFLLEARTAAGGPPVLTGDWYCDPDDRSVLIEWENGLTETVPLSADGRRLEGRGR
                     EGAAASTGGAGAADEAPTPESIAAAQEAAIDEGLDAYNRGDHATAYRLFRPIAEL"
     gene            734..>3079
                     /locus_tag="P5558_23335"
     CDS             734..>3079
                     /locus_tag="P5558_23335"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY27318.1"
                     /translation="MRQTARVVVIGGGVVGASVLYHLARAGWTDVLLVERDELTSGST
                     WHAAGGMHTLNSDPNVAKLQAYTIGLYREIEERSGQSCGVHLTGGLLLAGTPERMDFL
                     KAAHAKGRYLGMATELISAEEAARRMPLLDPAHFIGALFDPMEGHVDPYGVTHAYTKA
                     ARALGAEVVLRNRVVELRQRTDATWDVVTESGTVHAEHVVNAAGLWAREVGRMVGLEL
                     PVLAMEHQYLITEPMPEVAAAEKELLHVIDFEGEIYLRQEQGGMLMGTYERAGVPWSV
                     DSTPWDFSTRLLPEDLDRIAPSLEVGFRHFPAIGRAGIKKVVNGPFTFGPDGNPLVGP
                     VRSLRNYWCACAVMAGFSQGGGVGLALANWLVEGDPGFDVWAMDVARFGPFATPGYTQ
                     AKVRENYSRRFRITFPNEELAAARPLRTTPLYDLLVAENAVMGASFGQEHALWFAPPG
                     SEPVEHITFGRSNAFPVVAEECRAVREAVGLLETSNFAKYEVKGPGAEAWLGRVMAGR
                     LPAPGRITLSPMLNEAGRLIGDFTLARTGEERFLVFGSGVAETYHLRWFERHLPPSGV
                     GLRSVTSELLGVAIAGPRSRELLAGLTGADLSTGSFPFLACRRMEIDLVPVLVGRLSY
                     TGDLGYEIWVEAPCLRRVFLALREAGEAFGLRLFGARALNSLRLEKGFGSWAREYRPI
                     YGPREAGLDRFVDYTRGGFIGHAAAVAEKAERRLITLAVEAKDADVSGDEPIWHDGAV
                     VGWVTSGGYAHASRASVALGYVPADFAEAEAFEVEILGDRRP"
BASE COUNT          444 a         1015 c         1108 g          512 t
ORIGIN      
        1 cgagctcggc gatcggtcga aaaaggcgat aggcggtggc gtggtcgccg cggttgtagg
       61 cgtcgaggcc ctcgtcgatc gccgcctcct gcgccgcggc gatgctctct ggcgtcggcg
      121 cctcgtcagc cgcgcctgcg ccgccggtcg aggcggcggc gccctcgcga ccgcggccct
      181 cgaggcgccg gccgtcggcc gagaggggca cggtctcggt caggccgttc tcccactcga
      241 tgagcacgct ccggtcgtcg ggatcgcaat accagtcgcc ggtcagcacc ggcggccctc
      301 cggcggcggt ccgggcctcg agcaggaagt cgtcgcgcag ggtgacccgg ctgccatccg
      361 gccaccacca ggtgccgatc agcccgtcgc aggccgaggt cgccccggcg aggcccggca
      421 gcagcagtga gcccgcaagc gcgatcaaaa ttgcctgata gcacctcctc atgcatcgtt
      481 ctcttgacaa aagagttcag caaatcaatc agatcgggag ccgcaacgcc aattttttaa
      541 actgaaagtt caaaagaagc gcaagccatc tcatatttca agcttttata cggcattatt
      601 aacatgaata ctcgattgcg gcaaggtggc gtggccggcg tcgcatccgt tgtggcggag
      661 gtcgcttcgc gccactggcg accggcggcg ggtgcctgta gccttcaccg gtgttgccgg
      721 ggaggacggg tggatgcggc agacggcgcg ggtggtggtg atcggcggcg gcgtggtcgg
      781 ggccagcgtg ctctatcatc tggcccgggc cggctggacg gacgtgctgc tggtcgagcg
      841 cgacgagctc acctcgggct ccacctggca tgccgccggc ggcatgcaca cgctcaactc
      901 ggacccgaat gtcgccaagc tgcaggctta tacgatcggc ctctatcgcg agatcgagga
      961 acgctcgggg cagtcctgtg gcgtgcatct gaccggtggg ctgctgctcg ccggcacgcc
     1021 cgaacgcatg gacttcctta aagccgcgca tgccaagggc cgctatctcg gcatggcgac
     1081 cgagctgatc tcggccgagg aggcggcgag gcgcatgccg ctcctcgatc ccgcccattt
     1141 catcggcgcc ctcttcgatc cgatggaggg ccatgtcgac ccctatggcg tgacgcacgc
     1201 ctacaccaaa gccgcccggg cgctcggggc ggaggtggtg ttgcgcaacc gcgtggtgga
     1261 gctgcgccag cggacggatg cgacgtggga tgtggtgacc gagtccggca cggtgcatgc
     1321 cgagcatgtg gtcaatgcag cggggctctg ggcgcgcgag gtcgggcgga tggtcgggct
     1381 ggagctgccg gtgctcgcca tggagcacca gtacctgatc accgagccca tgccggaggt
     1441 ggcggcggcg gaaaaggagc tgctgcacgt catcgacttc gagggcgaga tctatctccg
     1501 ccaggagcag ggcggcatgc tcatgggcac ctacgagcgg gcgggggtgc cctggtcggt
     1561 cgattccacg ccctgggact tctcgacccg gcttttgccc gaggacctcg atcgcatcgc
     1621 gccgtcgctc gaggtcgggt tccggcattt cccggcgatc ggccgggccg gcatcaagaa
     1681 ggtcgtgaac ggccccttca ccttcggccc ggacggcaac ccactggtcg ggccggtccg
     1741 gtcattacgc aactattggt gcgcctgcgc cgtcatggcc gggttcagcc agggcggcgg
     1801 ggtggggctg gcgctggcca actggctggt ggagggcgat cccggcttcg acgtctgggc
     1861 gatggacgtc gcccgcttcg gcccgttcgc gacaccgggc tatacgcagg cgaaggtgcg
     1921 cgagaactac agccggcgct ttcgcatcac cttcccgaac gaggagctgg ccgccgcccg
     1981 gccgttgcgg accacgccgc tctacgacct gctcgtcgcc gagaatgccg tcatgggggc
     2041 gagcttcggc caggagcatg cgctctggtt cgccccgccc ggcagcgagc cggtcgaaca
     2101 catcaccttc gggcgctcga atgcctttcc cgtggtggcc gaggaatgcc gggcggtgcg
     2161 cgaggccgtg ggcctgctcg agacctccaa cttcgccaaa tatgaagtca agggcccggg
     2221 tgccgaggcc tggctcggcc gcgtcatggc gggccgcctg cctgcgcccg gccgcatcac
     2281 cctctcgccc atgctgaacg aggccggccg gctgatcggc gacttcaccc tcgcccgcac
     2341 cggcgaagag cgttttctcg tcttcggctc gggcgttgcg gagacgtacc atctgcgctg
     2401 gttcgagcgg catctgccgc cctcgggcgt cggcctgcgg tcggtcacgt cggaactcct
     2461 gggcgtggcc atcgccggcc cgaggagccg cgagctgctg gccgggctga ccggtgctga
     2521 tctctccacc ggcagctttc cctttctcgc ctgcaggagg atggagatcg acctcgtgcc
     2581 ggtgctcgtc ggccggctca gctacacggg cgatctcggc tacgagatct gggtcgaggc
     2641 gccctgtctg cggcgcgtgt tcctggcgct gcgcgaggcg ggcgaagcct tcggccttcg
     2701 tctcttcggg gcgcgggcgc tgaacagcct caggctggag aagggcttcg ggagctgggc
     2761 gcgggagtac cggccgatct acgggccgag ggaagcgggg ctcgatcgct tcgtcgacta
     2821 cacgagaggc gggttcatcg gccacgcggc ggccgttgcc gagaaggcag agcggcggct
     2881 catcacgctc gccgtcgagg cgaaggacgc cgatgtcagc ggcgacgagc ccatctggca
     2941 cgatggggcc gtggtcggct gggtcacctc gggcggctat gcccatgcca gccgggcctc
     3001 ggtggcgctg ggctacgtcc cggccgactt cgccgaggcg gaagcattcg aggtggagat
     3061 cctgggcgat cgtcggccg
//
LOCUS       DAOTFI010000378         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_37608_length_3075_cov_6.730795, whole genome shotgun sequence.
ACCESSION   DAOTFI010000378 DAOTFI010000000
VERSION     DAOTFI010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37608_length_3075_cov_6.730795"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1195
                     /locus_tag="P5558_23340"
     CDS             260..1195
                     /locus_tag="P5558_23340"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY27319.1"
                     /translation="MIRSLFLHWPWLLLAGLFGLTLAPILGDYSRKLDFLGQFLIQTT
                     ALTVAAFLLFLVLQHRLVAFAAVVCVILQLAVLQPALFPARAMTTSGESLSVVFANVW
                     TQNRRLPEVVERLEALDPDVIVLTEIREETESLLPALEATWPYRAACPRHYSCDTVVL
                     SRLPILEDRSGKGERGLVGLAAARIESGFGPVTVAGTHLTQPLPPWRQRVQEYQTEQV
                     VDMLAGVDDPLLVVGDFNSVPWGRLIRAFAAATGLKVAPGLEGTWPATLPWPLRIPID
                     QALTGRGLELLEREVVTMPGADHKALWLVVGPGTA"
     gene            complement(1200..2384)
                     /locus_tag="P5558_23345"
     CDS             complement(1200..2384)
                     /locus_tag="P5558_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY27320.1"
                     /translation="MVSESTQAKAGPRRRLRLGMVGGGQGAFIGGVHRIAARLDDRFE
                     LVAGCFASTAEKSKASGAELGIDPGRTYGSFADMAKAEAGRADGIDAVAIVTPNHVHY
                     PAAAAFLEAGIHVICDKPMTTTLADAAALAALVARSGLVFVLTHNYTGYPMIRQARAM
                     VAAGELGAIRVVQAEYAQDWLATKLEATGQKQAGWRTDPARSGPAGSTGDIGTHAMNL
                     AAFVTGLELDEVAAELSTFVEGRRLDDNCNVLLRYKGGARGMLWASQVATGNENALKL
                     RVYGDKGGLEWAQEDPNYLWFTALGEQKRLITRSGAGTGPEAGRVTRVPPGHPEGYLE
                     GFATIYSDAAELIWAAIEGRKPDDGARLAPSVQDGVAGVKFIEAVVASSGAGGAWTKA
                     AV"
     gene            complement(2421..>3075)
                     /locus_tag="P5558_23350"
     CDS             complement(2421..>3075)
                     /locus_tag="P5558_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRY27321.1"
                     /translation="HVGFTGALAFPYLYPWPQRPAGLVDEAFAELGRRWKPILDHYDS
                     CGVDYGYELHPGEDTFDGATFEMFLDAVGGHSRATINYDPSHFLLQQLDYLAFIDIYH
                     ERITSFHVKDAEFNPDGRQGVYSGYQPWTSRAGRFRSLGDGQVDFSGVFSKLTTHGYD
                     HWAVLEWECCLKHPEDGAAEGAPFIEDHIIRVTEKAFDDFAAGGSDQAANRRILGIG"
BASE COUNT          405 a         1088 c         1054 g          528 t
ORIGIN      
        1 cgtaggcatt ggaatgggcc ttgcccatga acccgtaacc gatcacggcg accctgagcg
       61 gcctcggcat gcttctttcc tccccggtgg ttcgagcgcg cttgcacctg cccttagcac
      121 gaccgccgcc gcgctggcag gggggctttg gtcttgcggc cgacatggca gcaagctgca
      181 ttgcggggtg cggtccgcga caagctggtt ctttctttgt taacggtccg gcgccatatt
      241 gcggcgcatg atccgcccct tgatccgctc cctcttcctg cactggccgt ggctgctgct
      301 ggccggtttg ttcggcctca cgctggcgcc gatcctcggc gactacagcc gcaagctcga
      361 cttcctcggc cagttcctga tccagaccac ggcactcacc gtggcggcgt tcctgctttt
      421 cctggtgctg cagcaccgcc tcgttgcgtt cgccgccgtc gtctgcgtca tcctgcagct
      481 cgccgtcctg cagccggccc ttttccccgc ccgggccatg accacgtccg gcgaaagcct
      541 gagcgtcgtg ttcgcgaacg tctggaccca gaaccgtcgg ctgcccgagg tcgtcgagcg
      601 gctcgaggcg ctcgatcccg atgtcatcgt gctcaccgag atacgcgagg agacggagtc
      661 ccttctgccg gcgctcgaag cgacctggcc ctatcgggcg gcctgcccgc gtcactacag
      721 ctgcgatacc gtcgtgcttt cccgcctgcc gatcctcgag gaccgcagcg gcaagggcga
      781 acgtggcctg gtcggcctcg ccgcggcacg aatcgagtcg ggcttcggcc cggtcaccgt
      841 cgccggcacg catctcacgc agccgctgcc gccctggcgc cagcgtgtcc aggagtacca
      901 gaccgagcag gtcgtcgaca tgctggccgg tgtcgacgac ccgctgctgg tggtcggcga
      961 tttcaactct gtgccctggg gtcgcctgat ccgcgccttc gcggcggcta ccggtctgaa
     1021 ggtggcgccg ggtctcgagg gcacctggcc cgcgaccctg ccgtggccgc tgcgcattcc
     1081 gatcgatcag gcgctgaccg ggcgcggcct cgagctgctc gagcgcgagg tggtgaccat
     1141 gcccggcgcc gatcacaagg cgctgtggct ggtggtcggc cccggcacgg cctgacggcc
     1201 tagactgcgg ccttcgtcca ggcgccgcct gcgccgctcg atgcgaccac ggcttcgatg
     1261 aacttcacgc ccgcgacacc gtcctgcacc gagggtgcga ggcgtgcgcc gtcgtccggc
     1321 ttgcggccct cgatcgcggc ccagatcagc tcggccgcgt cgctgtagat ggtggcgaag
     1381 ccttcgagat agccttcggg atggccgggc gggacgcggg tcacgcgacc ggcctcgggc
     1441 ccggtgccgg cgccggagcg ggtgatcagg cgcttttgct cgcccagggc cgtgaaccag
     1501 agatagttcg ggtcctcctg ggcccattcg agcccgccct tgtcgccgta gacccgcagc
     1561 ttcagcgcgt tctcgttgcc ggtcgccacc tggctcgccc agagcatgcc ccgggccccg
     1621 cccttgtagc gcagcagcac gttgcagttg tcgtcgagcc ggcggccctc gacgaaggtg
     1681 gagagctcgg ccgccacctc gtcgagctcg agcccggtga cgaaggcggc gaggttcatg
     1741 gcgtgggtgc cgatatcacc ggtcgagccg gccggcccgg agcgcgccgg gtcggtgcgc
     1801 cagcccgcct gcttctggcc ggttgcctcc agcttggtgg ccagccagtc ctgggcatat
     1861 tccgcctgca cgacgcggat ggcgccgagc tcgcccgcgg ccaccatcgc ccgggcctgg
     1921 cggatcatcg gatagcccgt gtagttgtgg gtcaggacga agacgagccc gctcctcgcg
     1981 acgagggcgg cgagggccgc ggcatccgcg agcgtcgtgg tcatgggctt gtcgcagatg
     2041 acgtggatgc cggcctcgag gaaggcggcg gcggccgggt agtggacgtg attgggcgtg
     2101 acgatcgcca ccgcgtcgat gccgtccgcc cggcccgcct cggccttggc catgtccgcg
     2161 aagctgccat aggtgcggcc cgggtcgatg ccgagctctg ccccggacgc cttcgacttc
     2221 tcggccgtgc tggcgaagca gccggcgacc agctcgaagc ggtcgtcgag ccgggcggcg
     2281 atgcgatgca cgccaccgat gaaggccccc tgcccgccgc ccaccatgcc gagccgcagc
     2341 cgccggcgcg gtccggcctt cgcctgtgtg ctctcgctga ccatgtctcg tctcccccct
     2401 ctctcgaccc ggcccgctgc tcagccgatc cccaggatgc gccggttggc tgcctggtcg
     2461 ctgccgcccg cggcgaaatc gtcgaaggcc ttctcggtaa cgcggatgat gtggtcctcg
     2521 atgaagggtg ccccctcggc cgcaccgtcc tcgggatgct tgaggcagca ctcccattcg
     2581 agcaccgccc agtggtcgta gccatgggtc gtgagcttcg agaagacgcc cgagaagtcg
     2641 acctggccgt cgcccagcga gcggaaccgc ccggcccggc tcgtccaggg ctggtagccg
     2701 gaatagacgc cctggcgccc gtccgggttg aactccgcgt ccttgacgtg gaagctcgtg
     2761 atccgctcgt gatagatgtc gatgaacgcc aggtaatcga gctgctgcag caggaagtgg
     2821 ctcgggtcgt agttgatcgt ggcccggctg tggccgccca cggcgtcgag gaacatctcg
     2881 aacgtggcgc cgtcgaacgt gtcctcgccc ggatgaagct cgtagccgta gtcgacgccg
     2941 caggaatcgt agtggtcgag gatcggcttc cagcgccggc cgagctcggc gaaggcctcg
     3001 tcgaccagac cggccggccg ctgcggccag gggtagagat agggaaaggc gagcgcgccg
     3061 gtgaagccga catgc
//
LOCUS       DAOTFI010000379         3065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_37778_length_3065_cov_6.328904, whole genome shotgun sequence.
ACCESSION   DAOTFI010000379 DAOTFI010000000
VERSION     DAOTFI010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37778_length_3065_cov_6.328904"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="P5558_23355"
     CDS             <1..466
                     /locus_tag="P5558_23355"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRY27322.1"
                     /translation="YTKSGTRPIVGMLKPGVKPTAKGLYLLDMIGDGPVRWGFPNIND
                     NAEITEMIACGAHLSLFSTGRGSVVGSAISPVIKVCANPETYRRMADDMDVDAGRILE
                     GRGTLDEVGREIVDLVTRVAGGEPTKSEALGHQEFVLTYKSFAPLGPACLPA"
     gene            576..2279
                     /locus_tag="P5558_23360"
     CDS             576..2279
                     /locus_tag="P5558_23360"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY27323.1"
                     /translation="MRDVFRIFMSSEGMVKWTVLAALILATMAEGIGLASMLPAVTLA
                     FGENDTTGIGQAVTDGLAWFGLPASLPMLLLVIVAGMLLKALLVILAMYHVSAVVAEV
                     ASRVRIALVTALLNVRWPYFTRHPVGRLANSVSLDSTRAGDAYLIATTYMATVFQAGI
                     TLVVALLVSWKVALVGLFVGAVIALILLPLVSMAKRAGLKQRQRTEDIVTLLSDTLSN
                     IKPLKAMSRQAQFAAFFASRTNKLRRALQQEAMSRHLMKSLREPIVAMAVAGAFYVVH
                     TSGAVALPELLVMGVLLSRIISTLGKVQELEQKAQNLQASYWSVHGLVAEAEAESEQF
                     TGTRRPTFEVGVALDKVTFGYGEKPVIQNASLTIPTGELTVITGPSGVGKTTITDLLL
                     GLLTPQSGAVRVDGVPLDELDVPAWRSMLGYVPQELALFHDSVLANVTLGDPSFDEKA
                     AEEALRLAGAWDFVAALPGGINQVVGERGTMLSGGQRQRIALARALVVKPKLLILDEV
                     TSALDPETEAAICANVRQLSGRVSIVAITHRPIWVELADRVYEVGPDGITTKEVSRAV
                     A"
     gene            2398..>3065
                     /locus_tag="P5558_23365"
     CDS             2398..>3065
                     /locus_tag="P5558_23365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27324.1"
                     /translation="MSTLLILGSKPEPALPAPGAIDAVACANASGRSAVRHGLPDPVI
                     TVMSAVLTSGHKAANDLALEALRGLRTGQLYIYPRPEAEGSALKRAWRELRRYRMHPW
                     YFKRRLRALDYGFDEAIAWSRERYDALMRRLTGGDPAVAELMARKQPSTGVMALAIGL
                     ADGRFDRFVMSGFSFEITHAYAHNPLIDEAGSTRSKHTETDVAILRCLAGRHAGIFTT
                     EPTV"
BASE COUNT          464 a         1055 c         1049 g          497 t
ORIGIN      
        1 ctacaccaag agcggcaccc ggccgatcgt cgggatgctc aagccgggcg tgaagccgac
       61 ggccaagggt ctctatctcc tcgacatgat cggcgacggc ccggtgcgct ggggcttccc
      121 gaacatcaac gacaatgccg agatcaccga gatgatcgcc tgcggagcgc atctctcgct
      181 cttctcgacc ggccgcggct cggtcgtggg ctcggccatc tcgccggtga tcaaggtctg
      241 cgccaacccc gagacctatc ggcggatggc ggacgacatg gatgtcgacg ccgggcggat
      301 cctcgaaggc aggggcacgc tcgacgaggt gggccgcgag atcgtcgacc tcgtgacgcg
      361 ggtggcaggt ggcgagccga cgaagtcgga ggcgctcggg catcaggagt tcgtgctgac
      421 ctacaagagc ttcgcgccgc tcggcccggc ctgcctgccg gcctgagcaa gcccggtggg
      481 gcaacagcgc cgcatggcga aattggcgtc gggtccccag atcgcctaga acgcgcgaca
      541 gcccccgcgc gtcacccctt cagcagccgg aatccatgcg cgacgtcttt cgcatcttca
      601 tgagctccga aggcatggtc aaatggaccg tgctggcggc gctcatcctc gccaccatgg
      661 ccgagggcat cgggctcgcc agcatgctgc ccgccgtgac cctggccttc ggcgagaacg
      721 acacgaccgg catcggccag gccgtcacgg acgggctcgc ctggttcggc ctgccggcga
      781 gcctgcccat gctgctcctg gtcatcgtcg ccggcatgct gctgaaggcg ctcctggtga
      841 tcctcgccat gtaccatgtg agcgccgtgg tcgccgaggt ggcgtcccgc gtgcggatcg
      901 ccctggtcac ggccctgctc aatgtccgct ggccctattt cacccgccat ccggtcggcc
      961 ggctggcgaa ctccgtgagc ctcgattcga cccgtgccgg cgacgcgtat ctgattgcca
     1021 cgacctatat ggcgacggtc ttccaggccg ggatcaccct cgtggtggct ctgctcgtct
     1081 cctggaaggt ggcgctggtc gggctctttg tgggtgcggt catcgccctg atcctcctgc
     1141 ctctggtcag catggcgaag cgcgccgggc tgaagcagcg ccagcgcacc gaggatatcg
     1201 tcacgctcct ctccgacacg ctctccaaca tcaagcccct gaaggcgatg tcgcggcagg
     1261 cgcagttcgc cgccttcttc gccagccgga ccaacaagct tcgccgggcg ttgcagcagg
     1321 aggcgatgag ccggcatctg atgaagagcc tgcgggagcc gatcgtcgcc atggcggtcg
     1381 ccggcgcctt ctacgtcgtc cacaccagcg gggcggtcgc cctgcccgag ctgctggtca
     1441 tgggcgtcct tctgtcccgg atcatcagca cgctcggcaa ggtgcaggag ctcgagcaga
     1501 aggcgcagaa cctccaggca tcctactggt cggtgcacgg gctggtggcc gaggccgagg
     1561 ccgagagcga gcagttcacc gggacgcgcc ggccgacttt cgaggtgggt gtcgccctcg
     1621 acaaggtgac cttcggctat ggcgagaagc cggtcatcca gaatgccagc ctgaccattc
     1681 caacaggcga gctcacggtc atcaccggcc cctccggcgt cggcaagacg acgatcaccg
     1741 atcttctgct cgggctgctc acgccacaga gcggggcggt gcgggtcgac ggggtgccgc
     1801 tcgacgagct ggatgtgccg gcctggcgct ccatgctggg ctacgtgccg caggagctgg
     1861 ccctcttcca cgacagcgtg ctggccaacg tgacgctggg tgacccgagc ttcgacgaga
     1921 aggcggccga ggaggcgctg cgcctggccg gcgcctggga tttcgtcgcc gccctgccgg
     1981 gcgggataaa ccaggtcgtc ggcgagcgcg gcaccatgct ctcgggcggc cagcgccagc
     2041 gcatcgccct cgcccgggcg ctcgtggtca agccgaagct cctgatcctc gacgaggtga
     2101 cgagcgccct cgatccggaa accgaggctg cgatctgcgc caatgtgcgc cagctcagcg
     2161 gtcgggtctc gatcgtcgcc atcacccacc ggccgatctg ggtggagctg gccgatcggg
     2221 tctacgaggt cgggcccgac ggcatcacga ccaaggaagt gtcgcgggcc gtggcctgag
     2281 agccaaatgg ggcacacccg ccccatgatc gccgggccgg ttgccgcacc tggttgcggg
     2341 tcgcggcgca catgctatct gacggggtgt gcctgccgcg ggttgaacga aggggccatg
     2401 tcgacacttc tgatcctggg ctcgaagccg gagccggcgc tgccggcgcc gggtgcaatc
     2461 gatgctgtcg cctgcgccaa tgcctcgggc cgctcggccg tgcggcacgg cctgccggat
     2521 ccggtcatca ccgtgatgtc ggcggtcctg acctccggtc acaaggcggc gaacgacctg
     2581 gcgctcgagg cgctgcgcgg cctgcgcacc ggtcagctct acatctaccc gcgaccggag
     2641 gcggagggct cggcgctgaa gcgcgcatgg cgcgagctgc ggcgctatcg gatgcacccc
     2701 tggtacttca agcggcggct gcgggcgctc gattatggct tcgacgaagc catcgcctgg
     2761 tcgcgcgagc ggtacgatgc gctgatgcgg cggctgacgg gtggcgatcc ggccgtggcg
     2821 gagctgatgg cgcgcaagca gccttcgacg ggggtgatgg ccctcgccat cggcctcgcc
     2881 gacggtcgct tcgaccggtt cgtgatgagc gggttctcct tcgagatcac gcacgcctat
     2941 gcccacaacc ccttgatcga cgaggcggga tcgacccgca gcaagcatac cgagaccgac
     3001 gtcgccatcc tgcgctgcct cgccggccgg catgccggca tcttcaccac cgagcccacg
     3061 gtgca
//
LOCUS       DAOTFI010000380         3051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_37984_length_3051_cov_8.214619, whole genome shotgun sequence.
ACCESSION   DAOTFI010000380 DAOTFI010000000
VERSION     DAOTFI010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37984_length_3051_cov_8.214619"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            674..2170
                     /locus_tag="P5558_23370"
     CDS             674..2170
                     /locus_tag="P5558_23370"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY27325.1"
                     /translation="MGGVSVAAAFDQAWRDPADSLLASRSGSSSGNPIASAGPETAPA
                     PDLAAAILPLAPGRDRGPASSIGLAAAEPTASEPAIAGPVLAQIEQSDSEPSTPGLAV
                     AAALVAPEAPPAEPAADATLADSTVEAEHLVVQRGDTLLDILTRSGIDRAEAHEVVGT
                     LSTVYDPRRLKVGQAMAVEVERAAGELAAPRLAALSLNLDFANDLRIERDADGSFAAR
                     TVDRELIETVHAARAVVRTSLYQTARQQNVPDEALISLANIFSWDVDFERDVHPGDAF
                     ELVYETVSSPDGETRGGEVLFASLSLRGRKMEAYRYVHGDGAVGYYDAEGRSLRKWLM
                     RTPIDGARLSSGFGKRRHPILGYNKMHRGVDFAAPTGTPIYAAGDGTLTQAGRNGSYG
                     NYIQIRHNGEYSTAYAHLSRFAKGITAGKRVRQGQVIGYVGTTGRSTGPHLHYEVLEN
                     GKQINPLAIKPQNAARLAGAELTRFRQQIETITTQRLPIEATTHLAQR"
     gene            2223..2579
                     /locus_tag="P5558_23375"
     CDS             2223..2579
                     /locus_tag="P5558_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HRY27326.1"
                     /translation="MTRVLTIYGIRSCDTMKKARAWLDARGLAHDFHDYKAKGIDRDR
                     LAAWVEQVGWESLLNRAGTTFRKLPEAEKQDLDTGKAIALMLAQPSMIKRPVLDVDGR
                     LLVGFKPEVYEAALGA"
     gene            complement(2674..>3051)
                     /locus_tag="P5558_23380"
     CDS             complement(2674..>3051)
                     /locus_tag="P5558_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRY27327.1"
                     /translation="EVMEVVRASFRPEFLNRLDEIILFNRLGRAQMGAIVEIQLGHLR
                     KLLTDRRIDLALTDQARTWLGNAGYDPVYGARPLKRVIQKNLQDRLATMILDGRLKDG
                     ETAHVEVKDGGLVIEPVIEAEAA"
BASE COUNT          498 a         1160 c          967 g          426 t
ORIGIN      
        1 gacacacgcc gcctgcacat cccctctcca tcattccaac ttgtcaaaag aacatgtgcc
       61 agcagcgatc agccgtaacg ccagggtttc ccgacgacgg cctcgccgcg acgaacggtg
      121 atatagcccc gcccttcggc ggctgcaagc cctttctcgc agaaaatccg aaggggccgg
      181 ccatgcccgt cacggcaccc ttccaggcgc gccggccacg ttcggaaaac ggcgggaaac
      241 gcccgtcagt actgcgatcg agggcatgcg gcaccctgaa tgaattgaca gcggccccgc
      301 cgccacagca tagtcgcggc cgagagaaca agccgtcggc tccgcccggc aggccggttt
      361 cgacagcgcc gggaatgccg ggccggatcg agagcgggtt gccgtcggat cggcaatttt
      421 cgtcgccttg gccgcaccag atcgacctgc ctgtgtggcg tatcgtgcga cagcaaccgt
      481 cgagcgtcgc tcccccggcg cccggctgta acgacagaga gccatattga tccgagcgtc
      541 gcgttccagg ccttccggac ttcagcccga cacagccgga ccaggccagc cggccgatag
      601 ccgtccggcc ggacgcgggc gatccagcga ttccgtcggc cgcctcgtcg tcaccctggc
      661 gctcggcacc ttcatgggcg gcgtctcggt ggccgccgcc ttcgatcagg cctggcgcga
      721 cccggcggac agccttctgg ccagccggtc gggctcttcg tcgggcaacc cgatcgcaag
      781 cgccggcccg gaaactgccc cggcacccga tctcgccgcg gccatcctgc cgctggcgcc
      841 gggccgcgat cgcggcccgg cctcgtccat cgggctcgcc gctgccgagc cgaccgcctc
      901 cgaaccggcc atcgccgggc cagttcttgc ccagatcgag cagagcgact cggagccgag
      961 cacccccggc ctcgccgtcg ccgcagcatt ggtggcgccc gaagccccgc ccgccgagcc
     1021 ggcagcggac gccactctcg ccgattccac cgtcgaggca gagcatctcg tcgtgcagcg
     1081 cggcgacacg cttctcgaca tcctcacccg gtcgggcatc gaccgcgccg aggcgcacga
     1141 ggtggtcggc acgctcagca ccgtctacga cccgcgccgc ctcaaggtcg gccaggccat
     1201 ggcggtggag gtcgagcggg cggccggcga gctcgccgcc ccgcggctcg ccgccctctc
     1261 gctcaatctc gacttcgcca acgacctgcg catcgagcgc gacgccgatg gcagcttcgc
     1321 cgcccggacg gtcgaccgcg agctgatcga gaccgtgcat gctgcccggg ccgtcgtccg
     1381 gaccagcctc taccagacgg cgcggcagca gaacgtgccg gacgaggcct tgatctccct
     1441 cgccaacatc ttcagctggg acgtcgattt cgagcgcgac gtccatcccg gcgacgcctt
     1501 cgagctggtc tacgagacgg tcagcagccc ggatggcgag acccgcggcg gcgaggtgct
     1561 gttcgccagc ctgtcgctgc gcggccgcaa gatggaagcc tatcgctacg tccacggcga
     1621 cggcgccgtc ggctactacg acgccgaggg ccgcagcttg cgcaaatggc tgatgcgcac
     1681 gccgatcgac ggggcgcgcc tctcctcggg cttcggcaag cgccggcacc cgatcctcgg
     1741 ctacaacaag atgcaccgcg gcgtcgactt cgccgccccc accggcacgc cgatctacgc
     1801 cgccggcgac ggcacgctga cccaggccgg ccgcaacggc tcctacggca actacatcca
     1861 gatccgccac aatggcgagt acagcaccgc ctatgcccat ctcagccgct tcgccaaggg
     1921 catcacggcc ggcaagcggg tccgccaggg ccaggtgatc ggctatgtcg gcacgaccgg
     1981 ccgctcgacc gggccgcatc tgcactacga ggtcctcgag aacggcaagc agatcaaccc
     2041 gctcgcgatc aagccgcaga acgcggcccg cctcgccggc gccgagctca cccgcttccg
     2101 ccagcagatc gagaccatca ccacccagcg cctgccgatc gaggcgacca cccacctcgc
     2161 ccagcgctga gccgggaccg accgacgcga cgagcggctg ccggacaggc gaagggccga
     2221 cgatgacccg cgtgctgacg atctatggca tcagatcctg cgacacgatg aagaaggccc
     2281 gggcctggct cgacgcgcgc ggcctcgccc atgactttca cgactacaag gccaaaggca
     2341 tcgaccgcga tcgcctggca gcctgggtcg aacaggtggg ctgggagtcc ctgctcaacc
     2401 gcgccggcac caccttccgc aagctgcccg aggcggagaa gcaagacctc gacaccggca
     2461 aggccatcgc cctcatgctg gcccagccct cgatgatcaa gcggccggtg ctggacgtgg
     2521 acggcaggct gctggtcggg ttcaagcccg aggtctacga agcggccctg ggggcgtaga
     2581 ggaaagcccg cgaggaggct cgcctcctcg cgctccaccc ttccctggca accgggggcg
     2641 cgggggaact cattcccccg cactttcccc tccttacgcc gcctcggcct cgatcacggg
     2701 ctcgatgacg agcccgccgt ccttgacctc gacatgcgcc gtctcgccgt ccttgaggcg
     2761 gccgtcgagg atcatggtgg cgagccggtc ctgcaggttc ttctggatca cccgcttgag
     2821 cggccgcgcg ccgtagaccg ggtcgtagcc cgcattgccg agccaggtgc gcgcctggtc
     2881 ggtgagcgcc aggtcgatgc ggcggtcggt gagcagcttc ctcagatggc cgagctggat
     2941 ctcgacgatc gcccccatct gcgctcggcc gagccggttg aagaggatga tctcgtccag
     3001 cctgttcagg aactccggcc ggaagctcgc ccgcaccacc tccatcacct c
//
LOCUS       DAOTFI010000381         3048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_38047_length_3048_cov_5.421316, whole genome shotgun sequence.
ACCESSION   DAOTFI010000381 DAOTFI010000000
VERSION     DAOTFI010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38047_length_3048_cov_5.421316"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..657)
                     /gene="mobA"
                     /locus_tag="P5558_23385"
     CDS             complement(16..657)
                     /gene="mobA"
                     /locus_tag="P5558_23385"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HRY27328.1"
                     /translation="MPPEPVFGLLLAGGQARRMGGGDKCLLAFAGRTLLARAIERLTP
                     QVGGLVLNANGDPARFADYGLPVLADVVPGFAGPLAGVLTGMAFVRQLDEPCDWLVTA
                     ATDTPFFPLDVVARLRAAAREAGADIAFARSADRVHPVFGLWRVALHDALAEALTEEG
                     ERKVDRFAARYRVATAGFSAVPFDPFFNINRPEDLAEAGQLSEPVNECPAPFR"
     gene            complement(647..1561)
                     /gene="fdhD"
                     /locus_tag="P5558_23390"
     CDS             complement(647..1561)
                     /gene="fdhD"
                     /locus_tag="P5558_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRY27329.1"
                     /translation="MATDTTGLRRSLVLPDPADPRLTRAVSGLDHEGRTVETRVIHER
                     PLTLFLNGQEIVTMMTIGDHPDYLAVGYLLNQNMLRPDDRITGIDYDEDLETVVVRTE
                     RATDYEEKLKKKMRTSGCAQGTVFGDLMEKFEDVRLPPDAVLRTSWLYALTKAINTCP
                     SLYLEAGAIHGCALCEGPDVLLYMEDVGRHNAVDKIAGYMFRHGLAAADKIFYTTGRL
                     TSEMVIKTVQMGIPILVSRSGFTAWGVELAQQAGLTLIGRARGRRFLALAGTGRIVFD
                     ADLSAVGEEEGRSARKGSRRDADAVDAA"
     gene            1683..>3048
                     /locus_tag="P5558_23395"
     CDS             1683..>3048
                     /locus_tag="P5558_23395"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRY27330.1"
                     /translation="MAKRSNGKAPAKPRRGLVRRLVRLLPALLVASLVVWVGLFVAYA
                     PELPDTDALFNDARQARVTLIAADGTVLAERGASGTAFVQLGEVSPVLRDAVIATEDR
                     RFYSHFGLDVRGTLRALLSNLRAGEVVAGGSTLTQQLAKNLFLTPDRSLRRKIQELIL
                     AFWLEARLTKDEILELYLNRVYLGAGAYGVEAAAQRYFGKPAARVDLAEAALLAGLLQ
                     APSRLAPTVDLDRANQRASTVLRLMVETGKITAEEAASARAAPARLAPAGGSELAGHF
                     VDWVLDGLTEHLGKPETDWVVRTTLDPRLQRVAEAAVADQLRDQPGIEAAVVLLDANG
                     AIRAMVGGRAYRRDRFNRASAGHRQPGSAFKPFVYLAALEDGHGPADVVEDLPLTVDG
                     WTARNPGGKRYGNVTLDEALALSINTVAVRLGQEVGMAKVAERARLLGIGSELRPVPS
                     LALGT"
BASE COUNT          442 a         1021 c         1136 g          449 t
ORIGIN      
        1 atcgctgcgg ctattctatc gaaaaggcgc aggacattca ttcacaggct ctgagagctg
       61 ccccgcctcg gccaggtcct cgggccggtt gatgttgaag aagggatcga agggcacggc
      121 cgaaaagccc gccgtggcga cgcggtagcg ggcggcgaag cggtcgacct tgcgctcgcc
      181 ttcctcggtc agcgcctcgg ccagggcatc gtgcaacgcc acgcgccaga ggccgaagac
      241 cgggtggacc cggtcggccg agcgggcgaa ggcgatgtcg gcgcccgctt cccgggccgc
      301 ggcgcgcagt cgggcgacga catcgagggg aaagaagggc gtgtcggtcg ccgccgtgac
      361 gagccagtcg cagggctcgt cgagctggcg cacgaaggcc atgccggtga gcacgccggc
      421 gagcgggccg gcgaagcccg gcacgacatc ggcgagcacc ggcaggccgt aatcggcgaa
      481 gcgggccgga tcgccattcg cgttgagcac gaggccgccc acctggggcg tcagccgctc
      541 gatggcacgg gcgagcaggg tgcggccggc gaacgcgagc aggcacttgt cgccgccgcc
      601 catgcgccgg gcctggccgc cggcgagcag gaggccgaag acgggctcag gcggcatcga
      661 cggcatccgc atctcttcgg ctgcccttgc gggcgctgcg cccctcttcc tcgccgacgg
      721 ccgaaagatc ggcatcgaag acgatccgtc cggtgccggc gagcgccaga aagcgccggc
      781 cgcgcgcccg gccgatgagc gtgaggcccg cctgctgcgc cagctcgacc ccccaggcgg
      841 tgaagccgga gcgcgagacg aggatcggaa tgcccatctg caccgtcttg atgaccatct
      901 ccgaggtcag gcggccggtg gtgtagaaga tcttgtcggc ggcggcgagg ccgtggcgga
      961 acatgtagcc ggcgatcttg tcgaccgcgt tgtggcggcc gacatcctcc atgtagagca
     1021 gcacgtccgg gccctcgcag agggcgcagc cgtggatggc gccggcctcg agatagagcg
     1081 aggggcaggt gttgatggcc ttggtgagcg cgtagagcca gctcgtccgg agcaccgcgt
     1141 ccggcggcag gcgcacgtcc tcgaacttct ccatgagatc accgaagacg gtgccctgcg
     1201 cgcagccgga ggtgcgcatc ttcttcttca gcttctcctc gtagtcggtg gcgcgctcgg
     1261 tgcggacgac gacggtctcg aggtcctcgt catagtcgat gccggtgatc cggtcgtccg
     1321 gccgcagcat gttctggttg aggaggtagc cgaccgccag atagtcggga tggtcgccga
     1381 tcgtcatcat cgtgacgatc tcctgaccat tgaggaagag ggtgaggggc cgctcgtgga
     1441 tcacccgggt ctcgactgtt cggccctcgt ggtcgaggcc gctgaccgca cgggtcaggc
     1501 gggggtcggc gggatcgggc aggacgaggc tgcgccggag gcccgtcgtg tcggtcgcca
     1561 tgcaggccgc ctcctcgtgc aacagtgatg cctttccgca acctaggtga tcgcagaccg
     1621 cttgcaatgc gccggtgctg ctgcgatcaa gccacgctcg gcaggatcgc gatgcgggag
     1681 cgatggcgaa acgttcgaat ggcaaggcac cggcgaagcc gcggcgcggc ctcgtcaggc
     1741 ggctggtgcg cctgctgccg gcgctcctcg tggcctcgct ggtggtctgg gtcgggctct
     1801 tcgtcgccta tgcgcccgag ctgccggaca cggatgcgct cttcaacgac gcgcgccagg
     1861 cgcgggtgac gctgatcgcg gccgacggca ccgtgctggc ggagcggggc gccagcggca
     1921 ccgccttcgt ccagctcggc gaggtcagcc cggtcctgcg tgatgcggtg atcgccaccg
     1981 aggaccggcg gttctacagc catttcgggc tcgacgtgcg cggcacgctc cgggcgctgc
     2041 tcagcaacct tcgtgccggc gaggtggtcg ccggcggcag cacgctgacc cagcagctgg
     2101 cgaagaacct cttcctcacc ccggaccggt cgctgcggcg caagatccag gagctgatcc
     2161 tggccttctg gctcgaggcg cggctgacga aggacgagat cctcgagctc tatctcaacc
     2221 gcgtctatct gggcgccggc gcctatggcg tggaggctgc cgcgcaacgc tatttcggca
     2281 agccggcggc ccgcgtcgat ctcgccgagg cggcgctgct cgccggcctc ctgcaggcac
     2341 cctcgcggct ggcgccgacg gtcgatctgg atcgcgccaa ccagcgtgcc tcgaccgtgc
     2401 tccggctgat ggtcgagacc ggcaagatca cggccgagga ggcggcctcg gcgcgagcgg
     2461 caccggcccg gctggcgccc gccggcggct cggagctggc cgggcatttc gtcgactggg
     2521 tgctcgacgg cctgaccgag catctcggca agcccgagac cgactgggtg gtgcggacca
     2581 ccctcgaccc ccgcctgcag cgggtggccg aggcggcggt ggcagaccaa ctgcgggacc
     2641 agcccgggat cgaggcggcc gtcgtgctgc tcgatgcgaa cggggcgatc agggccatgg
     2701 tcggcggccg cgcctatcgc cgcgatcgct tcaatcgggc cagcgccggg catcgccagc
     2761 ccggctcggc gttcaagccc ttcgtctatc tggcggcgct cgaggacggg cacggcccgg
     2821 ccgatgtggt cgaggacctg cccttgacgg tcgacggctg gacggcgcgc aatccgggcg
     2881 gcaagcgcta cgggaacgtg acgctcgacg aggcgctggc gctctccatc aacacggtgg
     2941 cggtccggct ggggcaggag gtcggcatgg cgaaggtcgc cgaacgggcg cggctcttgg
     3001 gcatcggcag cgagcttcgg ccggtgccgt cgctggcgct cggcactt
//
LOCUS       DAOTFI010000382         2950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_39754_length_2950_cov_6.324352, whole genome shotgun sequence.
ACCESSION   DAOTFI010000382 DAOTFI010000000
VERSION     DAOTFI010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39754_length_2950_cov_6.324352"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="P5558_23400"
     CDS             complement(<1..410)
                     /locus_tag="P5558_23400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY27331.1"
                     /translation="MAGSALLESDTVVVGGGIVGLCLAWFLATEGVEVAVLDDGSDAG
                     TTANAGSLHVQIQSRLIRRFPELLPGVEAALPMYPLAVRHWQRLTGLLPIDIDLRLTG
                     GLMVAEDDEQLDFLRAKAARERALGLEVEILDRRA"
     gene            complement(419..1381)
                     /locus_tag="P5558_23405"
     CDS             complement(419..1381)
                     /locus_tag="P5558_23405"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF004783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRY27332.1"
                     /translation="MEKIAPRPIAVIGAGSIGTAFAIVFGRAGHAVRLYDADPARLDP
                     AASLIEARLADLAAFDLLDEPTAVIASRIAGTADLAEALAGAVYVQECAPEDAALKRG
                     LFAELDRLAPPGAVLASASSAIPISTVAADLAGRARCLVVHPGNPPYLLPVAELVPAP
                     FTASATVDAAAALLEAAGMVPVRLEKEAEGFVFNRLQGAVLREAYCLVRDGVASVEAV
                     DAVMRAGLGRRWAVIGPFETADLNIRGGIEAHSRRMGPAYARMGAERGQDDPWTPELV
                     ARVAAERRARLPLDAWEARTAWRDRALMALERCRRSRPDLFEPG"
     gene            1491..2240
                     /locus_tag="P5558_23410"
     CDS             1491..2240
                     /locus_tag="P5558_23410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY27333.1"
                     /translation="MGQDFTGRTALVTGAATGFGRRIAELLATAGARGVAFDRVAHKP
                     PTGFVVVTGDVALEADLARGVAAAVERFGGLDLVVANAGIVPPWRETEALDLDEWDRV
                     FAVNVRGVAATLKHAVPALRAAGGGAIVAMASMNAARAHPRQCAYTASKHAVLGIVRA
                     TALDLGRHGIRVNAVAPGPVATAALVERMTSRAAAGGLAVEEALRRHAGETALGRMVT
                     EDDVARTVLHLLGPQSAGMTGQMLTVDAGLA"
     gene            2237..>2950
                     /locus_tag="P5558_23415"
     CDS             2237..>2950
                     /locus_tag="P5558_23415"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY27334.1"
                     /translation="MSGRRVETAGIEQAGEPVAFTFEGRRVEGLAGESLAAALTAAGI
                     VDWRGTRAGERRSQFCGMGVCQECLVQVDGRPAERACLTPVTAGLEVRRQSYAVDLAA
                     AAEPETRARAELAPDILVIGGGPAGLAAAEAASSAGAEVVLVDERASLGGQYFKPLAS
                     SHRFTGGATDAQFRAGLELVRRVEALPVRIIREALVWGAFGAGEIGVVAKGTSWLIRP
                     RRLILATGAYERGVPLPGWT"
BASE COUNT          380 a         1028 c         1173 g          369 t
ORIGIN      
        1 gcccggcggt cgaggatctc gacctcgagg ccgagggcgc gctcgcgtgc cgccttggcg
       61 cgcaggaagt cgagctgctc gtcgtcctcg gcgaccatga gcccgccggt caggcgaaga
      121 tcgatgtcga tggggagcag cccggtcagg cgctgccagt ggcggacggc caaggggtac
      181 atcggcaggg ccgcctcgac gcccggcaag agctcgggga agcggcggat gaggcggctc
      241 tggatctgca catgcaggct gccggcattg gccgtggtgc cggcgtcgct gccgtcgtcg
      301 aggaccgcca cctcgacccc ttccgtggcg aggaaccagg cgaggcagag cccgacgatg
      361 ccgccgccga cgacgacggt gtcgctctcg aggagcgccg atccggccac gcccgtggtc
      421 agcccggctc gaacaggtcg ggccgggacc ggcggcagcg ctcgagcgcc atcagcgccc
      481 ggtcgcgcca ggcggtgcgc gcctcccagg cgtcgagcgg caggcgggcc cggcgctcgg
      541 ccgccaccct ggccacgagc tcgggcgtcc aggggtcgtc ctggccgcgc tcggccccca
      601 tccgcgcata ggccgggccc atgcgccggc tgtgcgcctc gatgccgccc cggatgttga
      661 ggtcggccgt ctcgaacggc ccgatcaccg cccagcgccg gccgagcccg gcgcgcatca
      721 cggcatctac ggcctcgacc gaggcgacgc cgtcgcgcac caggcaatag gcctcgcgca
      781 gcacggcgcc ctgcagccgg ttgaagacga agccctcggc ctccttctcg agccgcaccg
      841 gcaccatgcc ggccgcctcg aggagggcgg ctgccgcatc cacagttgcg ctggcggtga
      901 agggcgccgg gacgagctcc gctacgggca agaggtaggg cgggttgccc ggatggacga
      961 cgaggcagcg cgcgcggccc gcgagatcgg cggcgacggt cgagatcggg atggcggagg
     1021 aggcgctggc gagcacggcc ccgggcggtg ccagccggtc gagctcggcg aagaggccgc
     1081 gcttgagggc cgcgtcctcg ggcgcgcatt cctggacata gacggcaccg gccagcgcct
     1141 cggcgaggtc ggccgtgccg gcgatgcggc tggcgatgac ggcagtcggc tcgtccagca
     1201 ggtcgaaggc ggcgaggtcg gcgagccggg cctcgatcag gctcgcggcc ggatcgagac
     1261 gggcgggatc cgcatcgtaa agccgcacgg catggccggc ccggccgaac acgatggcga
     1321 aagcggtgcc gatgctgccg gcgccgatca ccgcgatcgg ccgtggcgcg atcttttcca
     1381 agtggctgcc ctcgtctagc ttggtgttcg cagagcgcac gcttgtgctc ttctgcggag
     1441 gcggggcggt ggctgtcaag cggcggcccg ccgggcaagg ggatgcggga atggggcagg
     1501 acttcaccgg ccggacggcg ctggtcaccg gtgccgccac cggcttcggc cggcggatcg
     1561 cggagctgct ggcgacggcc ggggccaggg gcgtcgcctt cgaccgggtg gcgcacaagc
     1621 cgccgacggg cttcgtcgtc gtcaccggcg acgttgccct cgaggccgat ctcgcccgcg
     1681 gagttgcggc ggcggtcgag cgcttcggcg ggctcgatct cgtcgtcgcc aatgccggca
     1741 tcgtgccgcc ctggcgggag acggaggcgc tcgacctcga cgaatgggac cgggtcttcg
     1801 cggtcaacgt caggggcgtg gccgccacgc tcaagcatgc ggtgccggcg ctccgggccg
     1861 ccggcggcgg ggccatcgtc gccatggcgt cgatgaacgc ggcgcgggcg catccccggc
     1921 aatgcgccta cacggcgagc aagcacgcgg tgctcggcat cgttcgcgcc acggcgctcg
     1981 acctcggccg gcacggcatc cgcgtcaatg cggtggcccc ggggccggtg gcgacggcgg
     2041 ccctggtcga gcggatgacg agccgggcgg ccgctggcgg cctcgcggtc gaggaggcct
     2101 tgcgccggca tgccggggag acggcgctcg gccgcatggt gacggaggac gatgtggcgc
     2161 ggaccgtgct ccatctcctc gggccgcaat cggccggcat gaccgggcag atgctgaccg
     2221 tcgatgcggg gctggcttga gcggccggcg ggtcgagacc gccggcatcg agcaggccgg
     2281 cgagcccgtc gccttcacct tcgagggccg gcgggtggag gggctcgcgg gcgagagcct
     2341 cgccgcagcg ctcacggccg ccggcatcgt cgactggcgc ggcaccaggg cgggtgaacg
     2401 gcgcagccag ttctgcggca tgggggtctg ccaggaatgc ctggtccaag tcgacggcag
     2461 gccggccgag cgcgcctgcc tgacgccggt gacggccggg ctcgaggtgc gacgccaaag
     2521 ctacgccgtg gacctcgccg ccgccgccga gcccgaaacc agggcgcggg ccgagctggc
     2581 gccggacatc ctggtcattg gcggcgggcc cgccgggctc gccgcggccg aggcggcatc
     2641 gtctgccggg gcggaggtgg tgctggtcga cgagcgggcg agcctcggcg gccagtactt
     2701 caagcctctc gcctcgagcc atcgcttcac cggcggtgcc accgacgcgc agttccgggc
     2761 cggcctcgag ctggtgcgac gggtcgaggc gctgccggta cggatcatca gggaagcgct
     2821 ggtctggggc gccttcggcg cgggcgagat cggcgtcgtg gccaaaggca cgagctggct
     2881 catccggccg cggcggctga tcctcgccac cggcgcatac gagcgggggg tgcctttgcc
     2941 gggctggacc
//
LOCUS       DAOTFI010000383         2929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_40149_length_2929_cov_8.181280, whole genome shotgun sequence.
ACCESSION   DAOTFI010000383 DAOTFI010000000
VERSION     DAOTFI010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40149_length_2929_cov_8.181280"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="P5558_23420"
     CDS             complement(<1..1205)
                     /locus_tag="P5558_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY27335.1"
                     /translation="MTEHSQNTVFHSSSFLQGQNANYVEQLHARYVADPSSVDGSWQA
                     FFAGLGDADAEVTQNARGPSWARADWPPTPNDDLTAAMDGQWGPELVRAVGDKVRARA
                     AEAGVTLADAQVRQAVLDSVRALMIIRAYRIRGHLVADLDPLRLAEPEPHRELDPRSY
                     GFTEADMDRPIFIDNVLGLQTASLREILALVKRTYCGTFALQYMHISDPAQSAWLKER
                     IEGYGKEIRFTQQGRRAILNKLVEAEGFEKFLQVKYTGTKRFGLDGGEALIPAMEQII
                     KRGGALGVEEIVIGMPHRGRLSVLANVMEKPYRAIFNEFQGGSYKPDEVEGSGDVKYH
                     LGASSDREFDGNTVHLSLTANPSHLEAVDPVVLGKVRAKQSQMGDTSRSKVLPILLHG
                     DAAFAGQGVV"
     gene            complement(1297..2184)
                     /gene="sucD"
                     /locus_tag="P5558_23425"
     CDS             complement(1297..2184)
                     /gene="sucD"
                     /locus_tag="P5558_23425"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRY27336.1"
                     /translation="MSILVDENTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GGTHIGLPIFNTVAEAREKTGATATAIYVPPPFAADAILEAIDAGIELIVAITEGIPV
                     LDMMKVKRALQGSHSILIGPNCPGVITPDACKIGIMPGHIHKRGSVGVVSRSGTLTYE
                     AVGQTTAAGLGQSTCVGIGGDPIKGTEHIDVLEWFLSDPETESIIMIGEIGGSAEEDA
                     AQFLIDEKKKGRWKPTAGFIAGRTAPPGRRMGHAGAIVAGGKGGAEDKIEAMKSAGIV
                     VADSPAGLGEAVLKALRRK"
     gene            complement(2189..2506)
                     /locus_tag="P5558_23430"
     CDS             complement(2189..2506)
                     /locus_tag="P5558_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27337.1"
                     /translation="MVLTVFFTTLAALLAAPFIVVMLRAATPARVLRVWLAQLAAALA
                     LWGLLVLIFHSGLIVGQDPRILAALVINAVWVGLVLAMGFGTVAAVFRAVQLRARRRT
                     SGK"
     gene            complement(2526..>2929)
                     /gene="sucC"
                     /locus_tag="P5558_23435"
     CDS             complement(2526..>2929)
                     /gene="sucC"
                     /locus_tag="P5558_23435"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386312.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HRY27338.1"
                     /translation="LDGEIGCMVNGAGLAMATMDIIKLYGSEPANFLDVGGGATTEKV
                     TEAFKIITSDPNVKGILVNIFGGIMRCDVIAEGVIAAVKEVGLAVPLVVRLEGTNVEK
                     GKAILNESGLAVIAADDLADAAEKIVKAVKG"
BASE COUNT          425 a          997 c         1027 g          480 t
ORIGIN      
        1 accacgccct ggccggcgaa ggccgcgtcg ccgtgcagca ggatcggcag caccttggag
       61 cgcgaggtgt cgcccatctg cgactgcttg gcgcgcacct tgccgagcac caccgggtcg
      121 accgcctcga ggtggctggg gttggcggtg aggctgagat gcacggtgtt gccgtcgaac
      181 tcgcggtccg aggaggcgcc gagatggtat ttcacgtcgc ccgagccctc gacctcgtcg
      241 ggcttgtagc tgccgccctg gaactcgttg aagatcgccc ggtagggctt ctccatgaca
      301 ttggcgagca ccgagagccg gccgcggtgc ggcatgccga tgacgatctc ctcgaccccg
      361 agcgcgccgc cgcgcttgat gatctgctcc atcgccggga tcagcgcctc gccgccgtcg
      421 aggccgaagc gcttggtgcc ggtgtatttc acctgcagga acttctcgaa gccctcggcc
      481 tccacgagct tgttgaggat cgcccggcgg ccctgctggg tgaagcggat ctccttgccg
      541 tagccctcga tgcgctcctt gagccaggcg gattgcgccg ggtcggagat atgcatgtac
      601 tggagcgcga aggtaccgca ataggtgcgc ttgaccagcg cgaggatctc gcggagcgac
      661 gcggtctgca ggcccagcac gttgtcgatg aagatcggcc ggtccatgtc ggcctcggtg
      721 aagccgtagc tgcgcggatc gagctcgcga tgcggctcgg gctcggccag gcgcagcggg
      781 tcgaggtcgg ccaccagatg cccgcggatg cggtaggcgc ggatgatcat cagcgcgcgc
      841 accgaatcga gcaccgcctg gcggacctgc gcatcggcga gcgtcacgcc ggcctcggcc
      901 gcccgcgcgc gaaccttgtc gcccaccgcg cggacgagct ccgggcccca ttgcccgtcc
      961 atggccgccg tcaggtcgtc gttcggggtc ggcggccagt cggcgcgcgc ccaggagggg
     1021 ccgcgcgcgt tctgcgtcac ctcggcatcg gcatcgccga gccccgcgaa gaaggcctgc
     1081 cagctgccgt cgacgctgga gggatcggcg acgtagcgcg cgtgcagctg ctcgacgtaa
     1141 ttggcgttct ggccctggag gaaggacgag gaatggaaga ccgtgttctg cgaatgctcg
     1201 gtcatggctc tgacgggttg cccgctcctg cgatgcggcc cccgcggggg cctgtgctcg
     1261 aaaaccctgc cgcggggcgc ggcaggggca ttgctgtcac ttgcgccgca gcgccttgag
     1321 cacggcctcg ccgagaccgg cggggctgtc ggcgacgacg atgccggcgc tcttcatcgc
     1381 ctcgatcttg tcctcggccc cgcccttgcc gccggcgacg atggcgccgg catggcccat
     1441 gcgccggccg ggcggcgcgg tccggccggc gatgaagccg gcggtgggct tccagcggcc
     1501 cttcttcttc tcgtcgatca ggaactgcgc cgcgtcctcc tcggccgagc cgccgatctc
     1561 gccgatcatg atgatcgatt cggtctcggg atcggacagg aaccattcca gcacgtcgat
     1621 atgctcggtg cccttgatcg gatcgccgcc gatgccgacg caggtggact ggccgagccc
     1681 ggccgcggtg gtctggccca ccgcctcgta ggtcagggtt cccgagcgcg acacgacgcc
     1741 caccgagccg cgcttgtgga tatggcccgg catgatgccg atcttgcagg cgtccggggt
     1801 gatgacgccg gggcagttcg ggccgatcag gatcgaatgc gagccctgga gcgcgcgctt
     1861 gaccttcatc atgtcgagca ccgggatgcc ctcggtgatg gcgacgatca gctcgatccc
     1921 ggcgtcgatc gcctcgagga tcgcgtcggc cgcgaagggc ggcggcacgt agatcgccgt
     1981 cgccgtcgcg ccggtcttct cccgggcctc ggccacggtg ttgaagatcg gcaggccgat
     2041 atgggtgccg ccgccctttc cgggcgtcac gccgccgacc atcttcgtgc cgtaggcgat
     2101 ggcctgctcg ctgtggaagg tgccctggct gccggtgaag ccctggcaga tgaccttggt
     2161 gttctcgtcg acgaggatgg acatcagctc actttccgct tgttcgccgc ctggcgcgca
     2221 gctggacggc ccggaagacc gcagccacgg tcccgaagcc cattgccagc acgaggccga
     2281 cccagacggc attgatgacg agggcggcca ggatccgcgg atcctgcccg acgatgagcc
     2341 cgctgtggaa gatcagcacc agcaggcccc agagggcgag cgcggcggcg agctgggcga
     2401 gccagacccg cagcacccgc gccggcgtgg ccgcgcgcag catgaccacg atgaacggcg
     2461 cggcgagcag ggccgcgaga gtcgtgaaaa agaccgtcag gaccatggtc gcggccggac
     2521 atcgcctagc ctttcaccgc cttcacgatc ttctcggcgg catccgccag gtcgtcggcg
     2581 gcgatcaccg cgagccccga ttcgttgagg atcgccttgc ccttctcgac attggtcccc
     2641 tcgaggcgca ccaccagcgg caccgcgagg ccgacctcct tgaccgcggc aatcacgccc
     2701 tcggcgatga cgtcgcagcg catgatgccg ccgaagatat tgacgaggat gcccttcacg
     2761 ttcgggtcgg aggtgatgat cttgaaggcc tcggtcacct tctcggtggt ggcgccgccg
     2821 ccgacgtcga ggaagttggc gggctccgag ccgtagagct tgatgatgtc catggtcgcc
     2881 atggcgaggc cggcgccgtt gaccatgcag ccgatctcgc cgtcgagcg
//
LOCUS       DAOTFI010000384         2919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_40317_length_2919_cov_6.445880, whole genome shotgun sequence.
ACCESSION   DAOTFI010000384 DAOTFI010000000
VERSION     DAOTFI010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40317_length_2919_cov_6.445880"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="P5558_23440"
     CDS             complement(<1..143)
                     /locus_tag="P5558_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRY27339.1"
                     /translation="MSCIELAGSGGPEVLRPARRPIPSPGSNEVLIEVHAAGVNRPDV
                     LQR"
     gene            complement(212..427)
                     /locus_tag="P5558_23445"
     CDS             complement(212..427)
                     /locus_tag="P5558_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27340.1"
                     /translation="MTHFAQLPGSSRGTVAGHFLPPPRPTRWAVLYALALVGLPLVLA
                     LLLLDAALYLVFTRLLDRCYGLLCFLS"
     gene            452..1387
                     /locus_tag="P5558_23450"
     CDS             452..1387
                     /locus_tag="P5558_23450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY27341.1"
                     /translation="MDGIILMHWRLDFLDEPAWRRLLASAEHPALQQSWGYGVAVAGQ
                     GHAVVRAVLHDGERCLALAQVVRRRLPCGWGVGLLLRGPVWLEAPDAERETVAIDRLR
                     AALGRTLLVWQPDDADAGERRAGWRRVWTGPSTAWLDLTPPAALLRSRLDAKWRNRLV
                     QAEATSMSAIEAVGGSDFEWLVEANERQRLRVGYRGPSAGFIRALAAAGRRGMMALIA
                     RHEGAPVAGVLLARHGRAATYYAGVTTAAGRQLRAHHRLLWEGILRLQRAGCTALDLG
                     GIDTVTSPGIARFKLGLGATPLTLAGSYLVAPRLR"
     gene            complement(1455..>2919)
                     /gene="groL"
                     /locus_tag="P5558_23455"
     CDS             complement(1455..>2919)
                     /gene="groL"
                     /locus_tag="P5558_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423234.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRY27342.1"
                     /translation="FENMGAQMVREVASKTNDVAGDGTTTATVLTQAIVREGLKSVAA
                     GMNPMDLKRGIDKAVAQVMEEIKKKSKKVKNSEEIAQVGTISANGEASIGQMIAAAMQ
                     RVGNEGVITVEEAKSLETELDVVEGMQFDRGYLSPYFITDSEKMRAVLEDPYILIHEK
                     KLSNLQTLLPVLEAVVQSAKPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGD
                     RRKAMLEDIAILTAGQVISEDLGIKLENVTLDMLGHAKRVVINKEDTTIVDGAGGKEE
                     ISGRIAQIKAQIEDTTSDYDREKLQERLAKLAGGVAVIRVGGATEIEVKERKDRVDDA
                     MNATRAAVEEGIVPGGGVALLQAMKALKTLKADNADMQFGVDIVRKAIQSPARQIAEN
                     AGVDGAVIVGKLLESTETNWGYDAYEGVFCDLVKKGIIDPTKVVRTALQDAASVAGLL
                     VTTEAMVAEKPERKSAPAGGGDMGGMGGMGGMGGMDF"
BASE COUNT          408 a          994 c         1018 g          499 t
ORIGIN      
        1 tgtcgctgca gcacgtccgg ccggttgacg cccgcggcat gcacctcgat cagcacctcg
       61 ttcgacccgg gcgacgggat gggccggcgg gccgggcgca ggacctccgg gccgcccgat
      121 ccggcgagct cgatgcacga catgtcaccc tggatcaccc ggcctgctcc tctcgatcgc
      181 ttcggactga gccacaacct agcgctggtc ctcaggaaag gaagcagagg agtccgtagc
      241 agcggtcgag gaggcgggtg aagacgagat agagggctgc atcgagcagc aggagggcga
      301 ggacgagcgg caggccgacc agtgcgaggg catagaggac ggcccagcgc gtcggccggg
      361 gcggcggcag gaaatgcccg gcgacggtgc cgcgggacga accgggtagt tgcgcaaagt
      421 gtgtcatatt tctacttata cgagaaatac ggtggacggc atcattctca tgcactggcg
      481 cctcgatttc ctcgacgagc cggcttggcg gcggctcctg gcctcggccg agcaccctgc
      541 cctgcagcag tcctggggct atggtgtcgc cgtggccggc cagggccatg cggtggtgcg
      601 cgccgtgctg cacgacggcg agcgctgcct tgccctggcg caggtggtgc gccggcggct
      661 gccttgcggc tggggcgtcg gcctcttgct gcgcggcccg gtctggctcg aggcgccgga
      721 cgccgagcgc gagacggtgg ccatcgaccg gctgcgggca gcactcgggc gcacgctcct
      781 cgtctggcaa ccggacgacg ctgacgctgg cgaacggcgt gccggctggc gccgtgtctg
      841 gaccgggccg agcacggcct ggctcgacct gacgccgccg gcggcgctgt tgcgcagccg
      901 gctcgacgcc aagtggcgaa acaggctcgt ccaggccgag gcgacgtcaa tgagcgccat
      961 tgaggcggtc ggcggcagcg atttcgagtg gctggtcgag gccaacgagc ggcagcgcct
     1021 gagggtgggc taccgggggc cgagcgccgg cttcatccgc gcgctggctg cggccgggcg
     1081 gcgcgggatg atggcgctga tcgcccgcca cgagggagca ccggtggccg gcgtgctgct
     1141 ggcccggcac ggccgggcgg cgacctacta tgccggcgtg accacggcgg cagggcgcca
     1201 attacgcgcc catcaccggc tgctatggga gggcatcctc cgcctgcagc gggccggctg
     1261 cacggcgctg gatctcggcg gcatcgatac cgtcaccagc cccggcatcg cccgcttcaa
     1321 gctcgggctc ggcgccacgc cgctgacgct cgccggcagc tatctcgtgg caccccggct
     1381 gcgttgaaaa gagaaagggg ccgcgccagc ggcgcgaccc cctctcccga ttggtaggtt
     1441 cgggctgtct ggacttagaa gtccatgcca cccatgccgc ccatgccacc catgccgccc
     1501 atgtcaccac cgccggcggg agccgacttc cgctcgggct tctcggcgac catggcctcg
     1561 gtcgtcacga gcaggccggc gaccgaagcc gcgtcctgca gcgcggtgcg gacgaccttg
     1621 gtcgggtcga tgatgccctt cttgacgagg tcgcagaaca cgccctcata ggcatcgtag
     1681 ccccagttcg tctcggtcga ctcgagcagc ttgccgacga tcacggcacc atcgacgccg
     1741 gcgttctcgg cgatctgcct ggccggggac tggatcgcct tgcggacgat gtcgacaccg
     1801 aactgcatgt cggcgttgtc ggccttgagc gtcttcaggg ccttcatcgc ctgcagcagc
     1861 gccacgccgc cacccggcac gatgccttcc tcgaccgcgg cgcgggtcgc gttcatcgcg
     1921 tcgtcgacgc gatccttgcg ctccttcacc tcgatctccg tggccccgcc gacgcggatg
     1981 acggcaacgc cgccggccag cttggcgagc cgctcctgca gcttctcgcg gtcgtagtcc
     2041 gaggtggtgt cctcgatctg cgccttgatc tgggcgatgc ggccggagat ctcctccttg
     2101 ccaccggcgc cgtcgacgat ggtggtgtct tccttgttga tcaccacgcg cttggcatgg
     2161 ccgagcatgt cgagggtgac attctcgagc ttgatgccga ggtcctcgct gatcacctgg
     2221 ccggcggtca ggatggcgat gtcctcgagc atggccttgc gccggtcacc gaagccgggc
     2281 gccttgacgg cggcgacctt gagaccgcca cgcagcttgt tgacgacgag ggtggcgagc
     2341 gcctcgccct cgatgtcctc ggcgatgatc aggagcggct tggcgctctg gacgaccgcc
     2401 tcgagcaccg gcagcagggt ctgcaggttc gagagcttct tctcgtggat caggatgtag
     2461 ggatcctcga ggacggcgcg catcttctcg ctgtcggtga tgaagtaggg cgagaggtag
     2521 ccgcggtcga actgcatgcc ctcgacgacg tcgagctcgg tctcgaggct cttggcctcc
     2581 tcgaccgtga tcacgccctc gttgcccacg cgctgcatgg ccgcggcgat catctggccg
     2641 atcgacgcct caccgttggc cgagatcgtg ccgacctggg cgatctcttc cgagttcttg
     2701 accttcttcg acttcttctt gatctcctcc atgacctggg cgacggcctt gtcgatgccg
     2761 cgcttgaggt ccatggggtt catgccggcg gcgacggact tcaggccctc gcgaacgatc
     2821 gcctgcgtca ggacggtggc cgtcgtcgtg ccgtcaccgg ccacgtcgtt ggtcttcgag
     2881 gccacttcgc gcaccatctg ggcgcccatg ttctcgaac
//
LOCUS       DAOTFI010000385         2903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_40597_length_2903_cov_9.036517, whole genome shotgun sequence.
ACCESSION   DAOTFI010000385 DAOTFI010000000
VERSION     DAOTFI010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40597_length_2903_cov_9.036517"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="P5558_23460"
     CDS             <1..1563
                     /locus_tag="P5558_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRY27343.1"
                     /translation="RDVGLEAEAPAFGVAADRTGSGITLKELPTDRDPAKAKAFIDFQ
                     NDVTAKDIRLAVREGMRSIEHVKRYTTNGMATDQGKMSNINGLMIASDALGKAPPQVG
                     LTTFRPPYSPTTFGTFAGYHQDATFEVTRKTPIDPWAEANGAVFEPVALWRRAWFFPK
                     PGEDMHAAVGRECRGARASVGIFDASTLGKIEVVGPDAVTFMERMYTNPWAKLGVGRC
                     RYGLLLGEDGFIRDDGVIGRIAEDRFHVTTTTGGAARVLNMMEDYLQTEWPDLKVWLT
                     STTEQWAVVALQGPNARKLLEPFVERLDISAEAFPHMAVAECTVAGFPARLFRVSFTG
                     ELGFEVNVPARHGRALWETLWQAGQQYDICPYGTETMHVLRAEKGYIIVGQDTDGTLT
                     PGDAGLTWAIGKAKPDFVGKRSLTRPDMVAKGRKQLVGLLTEDPKVVLQEGAQIVADP
                     AQPKPMTMIGHVTSSYWSDALGRSIAMAVVVDGRERDGQTLHIPMPGGTITAKVVKST
                     AFLDPENRRLSA"
     gene            1578..2045
                     /locus_tag="P5558_23465"
     CDS             1578..2045
                     /locus_tag="P5558_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase"
                     /protein_id="HRY27344.1"
                     /translation="MTATTDPGVTVTELPPPTRITLRVADPAGQPLPTRIGAIEGCAL
                     CLGPDEWLLEGDAAADLVAGLPGPYSAVEVSDREITYLLEGPAVLDLLATGCPRDLAS
                     MPVGAGARTVYDTAQVVLVREANDRFRLTVWRSFAPHVRALFDIAMREIAAGL"
     gene            2056..2903
                     /gene="glyA"
                     /locus_tag="P5558_23470"
                     /pseudo
     CDS             2056..2903
                     /gene="glyA"
                     /locus_tag="P5558_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388897.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
BASE COUNT          474 a         1082 c          926 g          421 t
ORIGIN      
        1 cgcgacgtcg gcctcgaggc ggaggctcca gccttcggcg tggccgccga ccgcaccggc
       61 tcgggcatta cgctgaagga actcccgacc gaccgcgacc cggccaaggc caaggccttc
      121 atcgacttcc agaacgacgt gacggccaag gacatccgcc tggccgtgcg cgaggggatg
      181 cgctcgatcg agcacgtcaa gcgctacacg acgaacggca tggcgaccga ccagggcaag
      241 atgtcgaaca tcaacggcct gatgatagcc tcggacgcgc tcggcaaggc gccgccccag
      301 gtcggtctga ccactttccg gccgccctac tcgccgacga ccttcggcac cttcgccggc
      361 taccaccagg acgcgacctt cgaggtcacc cgcaagacgc cgatcgaccc ctgggccgag
      421 gcgaacggcg cggtcttcga gccggtcgcc ctctggcgcc gcgcctggtt cttcccgaag
      481 ccgggcgagg acatgcacgc ggcggtcggt cgcgagtgcc gcggcgcccg cgcctcggtc
      541 ggcatcttcg acgcctctac cctcggcaag atcgaggtcg tcggccccga cgccgtgacc
      601 ttcatggagc ggatgtacac gaacccctgg gcgaagctcg gggtcgggcg ctgccgctac
      661 ggcctgctgc tcggcgagga cggcttcatc cgcgacgacg gcgtcatcgg ccggatcgcc
      721 gaggaccgct tccacgtcac gacgacgacc ggcggcgcgg cgcgcgtgct gaacatgatg
      781 gaggactacc tgcagaccga gtggccggac ctcaaggtct ggctcacctc gacgacggaa
      841 cagtgggcgg tcgtggcgct gcaggggccg aacgcccgca agctgctcga gcctttcgtc
      901 gagaggctcg acatctcggc cgaggcattc ccgcacatgg cggtcgcgga atgcaccgtc
      961 gccggcttcc cggcccggct gttccgcgtc tccttcaccg gcgagctcgg cttcgaggtc
     1021 aacgtccccg cccgccacgg ccgcgccctc tgggagacgc tctggcaggc cggccagcaa
     1081 tacgacatct gcccctacgg caccgagacc atgcatgtcc tgcgcgcgga gaagggctat
     1141 atcatcgtcg gccaggacac cgacggcacg ctgacgcccg gcgacgccgg cctgacctgg
     1201 gcgatcggca aggcgaagcc cgatttcgtc ggcaagcgct cgctgacccg ccccgatatg
     1261 gtggcgaagg gccgcaagca gctggtcggc ctcctgaccg aagacccgaa ggtcgtgctg
     1321 caggagggag cgcagatcgt ggcagacccg gcccagccga agccgatgac gatgatcggc
     1381 cacgtcacct cctcctactg gagcgacgcg ctcggccgct cgatcgccat ggccgttgtc
     1441 gtcgacggcc gggaacggga cgggcagacg ctgcacatcc cgatgcccgg cggcaccatc
     1501 accgcgaagg tggtgaagag caccgccttc ctcgaccccg agaaccgccg gctgagcgcc
     1561 tgagaggagt aggagcgatg actgcaacca ccgaccccgg cgtgaccgtc accgagctgc
     1621 cgccgccgac ccgcatcacg ctgcgggtcg ccgatccggc cggccagccc ctgccgaccc
     1681 gcatcggtgc gatcgagggc tgcgccctct gcctcggccc ggacgaatgg ctgttggaag
     1741 gcgacgccgc cgcggatctc gtcgccgggt tgccagggcc ctacagtgcc gtcgaggtct
     1801 ccgaccgcga gatcacctac ctgctcgaag gcccggccgt cctcgacctg cttgccaccg
     1861 gctgcccacg cgacctcgcg tcgatgccgg tcggcgcagg tgcccgtacc gtctacgaca
     1921 cggcgcaggt cgtcctcgtc cgcgaggcga acgaccgctt ccgactcacc gtctggcgct
     1981 cgttcgcgcc gcatgtccgc gccctcttcg acatcgccat gcgcgagatc gccgcaggtc
     2041 tataagggaa ctcccatgct cgacgccgac gcctcccgcc tttccgacga tgccgttgcc
     2101 gccgcaatcg gccgcgacct cggccgccag cagcaccaga tcgagctgat cgcctcggag
     2161 aacatcgtct cccgcgacgt gcagatcgcc cagggctcgg tgctgaccaa caagtacgcc
     2221 gagggctatc ccggcaagcg ctactaaggc ggctgcgagt tcgtcgacga ggtcgagacc
     2281 atcgcgatcg accgcgtgta gaagctcttc ggcgccgtct acgccaacgt tcagccgcac
     2341 tcgggcgccc aggcgaacca ggcggcgttt ctctcccttc ttcagcccgg cgaccgtttc
     2401 atgggcatgt cgctcgccca cggcggccac ctgacccacg gctcgccggt caccatgccc
     2461 ggcaagtggt tcgacgtcgt cgactaccaa gtccgcgatc agaaccagat gatcgacatg
     2521 gactccgtcc gcgagcgcgc gctggagacc aagccgaagc tcatcatcgc cggtgcctcg
     2581 gcctatccgc gccagatcga cttcaccggc ttgcgcaaga tcgccgagga agttggcgcg
     2641 tacctgatgg tcgacatggc gcattacgcc gggctgattg ctgccggcct ccacccgaat
     2701 ccggtgccgc acgcccgtgt cgtcacctcg atcacccaca agaccctgcg cggcccccgc
     2761 ggcggcggca tcctcaccaa cgacgcggat cgggcgaaga agctctactc cgcggtcttc
     2821 cccggcaacc agggcggtcc gctgatgcac gtcgtcgccg ccaaggccgt cgccttcggc
     2881 gaggcgctgc agccatcgtt caa
//
LOCUS       DAOTFI010000386         2887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_40887_length_2887_cov_6.536723, whole genome shotgun sequence.
ACCESSION   DAOTFI010000386 DAOTFI010000000
VERSION     DAOTFI010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40887_length_2887_cov_6.536723"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="P5558_23475"
     CDS             <1..666
                     /locus_tag="P5558_23475"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRY27345.1"
                     /translation="GAGVLAQELAQTLLGALIALNRGFVGALRDQDAGRWRPWSFAAL
                     GGQTLVIVGTGAIGTAFAALAKALGLRVIGVARTAAPKPPFDRVVPLADLASIAAEAD
                     YLSINVRLTEATRHLIDAALLDAMRPSAFLLNASRGAVVDEAALIRALDERRLAGAYL
                     DVFETEPLPESSPFWRLDNVLITPHCSDRVADWELCHARFFMANLERRLAGQELLNRV
                     AGD"
     gene            complement(667..2265)
                     /locus_tag="P5558_23480"
     CDS             complement(667..2265)
                     /locus_tag="P5558_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRY27346.1"
                     /translation="MSEIDDRQRLTDEAGRRRTFAIISHPDAGKTTLTEKLLLFGGAI
                     HLAGEVKARGDRRRARSDWMSIEQKRGISVTTSVMTFDYGGCTFNLLDTPGHEDFSED
                     TYRTLTAVDSAVMVIDAAKGIEAQTLKLFEVCRLRDMPIMTFINKLDREARDPFELLD
                     EIEERLALDPSPMNWPIGMGQNFRGVAERATGDLLLLKPEGRGTDTEERVKRRSNTVG
                     ERIDPRQLEKLDEELDLVEGALEPFDHDAYRQGNLTPVFFGSALRTFGIRALLDQLVA
                     EAPSPRPQPAEPAPVAPDEPQVAGFVFKVQANMDPKHRDRVAFVRLCSGRFRRGMKLK
                     MVRHQKPLAVANPIFFFAQDRGLAEEAFPGDIIGVPNHGQLRVGDALCETEDRRFTGI
                     PSFAPEILMRVRLGDPMRAKHLRKALEDLAEEGVTQVFRPRLGAEWIVGVVGQLQLDV
                     LKSRLEAEYGLPIDFEGAPYVTARWCTAPDQAELERFEQAKKASLADDRDGQPVFMAR
                     NSWDLGRVQEDFPKIAFHKTRERA"
     gene            2467..>2887
                     /locus_tag="P5558_23485"
     CDS             2467..>2887
                     /locus_tag="P5558_23485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY27347.1"
                     /translation="MTPPASAQNGLDPENTLLIELEAGTVTVELLPDVAPQHVARVKE
                     LAREGFYDGVVFHRVIDGFMAQTGDPTGTGTGGSSKPDLKAEFSKEPFKRGTIGAART
                     QNPNSANSQFFICFQPAPHLNGQYTVWGRVVDGMELVD"
BASE COUNT          424 a         1013 c          982 g          468 t
ORIGIN      
        1 ggcgccggcg tgctggcaca ggagctcgcc cagaccctgc tcggcgccct catcgccctc
       61 aatcgcggct tcgtcggcgc cctgcgcgac caggatgcgg gacgctggcg accctggagc
      121 ttcgccgccc tcggcgggca gacgcttgtg atcgtcggca ccggggcgat cggcacggcc
      181 ttcgcggccc tggcgaaagc gcttggtctc agggtcatcg gtgtcgcccg gacggctgcg
      241 ccaaagccgc ctttcgatcg ggtcgtgccg ctcgccgatc tcgcctcgat cgccgccgag
      301 gcggactatc tcagcatcaa tgtccggctg accgaggcca cgcggcacct gatcgatgca
      361 gccttgctcg acgccatgcg gccttccgcc tttctcctca acgcgtcgcg gggggccgtg
      421 gtcgacgagg cggcgctgat ccgggccctc gacgagcggc gccttgccgg cgcctatctc
      481 gacgtgttcg agaccgagcc cttgcccgaa tcgagcccct tctggcggct cgacaacgtg
      541 ctgatcacgc cgcattgcag cgaccgggtc gccgactggg agctgtgcca tgcccggttc
      601 ttcatggcca atctcgagcg ccggctggcc gggcaggagc tcctcaaccg cgtggcgggc
      661 gactgatcag gcgcgctcgc gcgtcttgtg gaaggcgatc ttcgggaaat cctcctgcac
      721 ccggccgagg tcccagctgt tgcgggccat gaagaccggc tggccgtcgc ggtcgtcggc
      781 gaggctcgcc ttcttcgcct gctcgaaccg ctcgagctcg gcctggtccg gggcggtgca
      841 ccagcgcgcg gtcacgtagg gcgcaccctc gaagtcgatc ggcaggccgt actcggcctc
      901 gagcctggac ttcagcacgt cgagctgcag ctggccgacc acaccgacga tccattccgc
      961 ccccagccgc gggcggaaca cctgcgtgac gccctcctcg gccaggtcct cgagcgcctt
     1021 cctcagatgc ttggcgcgca tcgggtcgcc gagcctgacg cgcatcagga tctcgggcgc
     1081 gaagctcgga atgccggtga agcggcggtc ctcggtctcg cagagcgcgt cgccgacccg
     1141 gagctggccg tgattgggca cgccgatgat gtcgcccggg aaggcctcct cggcgaggcc
     1201 ccggtcctgg gcgaagaaga agatcgggtt ggcgacggcg agcggcttct ggtggcgcac
     1261 catcttcagc ttcatgccgc ggcgaaagcg ccccgagcag aggcgcacga aggcgacgcg
     1321 gtcgcgatgc ttggggtcca tgttggcctg gaccttgaag acgaagcccg cgacctgcgg
     1381 ctcgtccggt gcgaccggcg ccggctcggc cggctgcggc cgcggcgagg gcgcttcggc
     1441 gacgagctgg tcgaggaggg cgcggatgcc gaaggtgcgg agcgcactgc cgaagaagac
     1501 cggggtcagg ttgccctggc gataggcgtc gtggtcgaag ggctcgaggg ccccctcgac
     1561 gagatccagc tcctcgtcga gcttctcgag ctggcgcggg tcgatgcgct cgcccacggt
     1621 gttgctccgc cgcttcacgc gctcctcggt gtcggtgccg cgcccctccg gcttcaggag
     1681 caggaggtcg ccggtcgccc gctcggccac gccgcgaaaa ttctggccca tgccgatcgg
     1741 ccagttcatc gggctcggat cgagcgccag ccgctcctcg atctcgtcga gcagctcgaa
     1801 ggggtcgcgg gcctcgcggt cgagcttgtt gatgaacgtc atgatcggca tgtcgcgcag
     1861 ccggcagact tcgaagagct tgagggtctg cgcctcgatg cccttggcgg cgtcgatcac
     1921 catgacggcg ctgtcgacgg cggtcagcgt ccggtaggtg tcctccgaga agtcctcgtg
     1981 acccggcgtg tccaggaggt tgaaggtgca gccgccatag tcgaaggtca tgaccgaggt
     2041 ggtgaccgag atgccgcgct tctgctcgat cgacatccag tccgagcgcg cccggcgccg
     2101 gtcgccccgc gccttgacct cgccggccag atgaatggcg ccgccgaaca gcaggagctt
     2161 ctcggtcagc gtcgtcttgc cggcatcggg atgcgagatg atggcgaagg tgcggcggcg
     2221 gcccgcctcg tcggtcaggc gctggcgatc atcgatttcg gacaaatggc cctcgagcat
     2281 acggccggct cggccgatcc tcggccgccg ggacggaaaa cgatggacac cgcctagcgc
     2341 gaaacggccc gagccgctat gtccccgcct tcaggtggcc ccacccgtat gaacatcaag
     2401 ggacacggac acgtgcaact catgcgcaat cttcttctca tccttttcgg aggcctcgct
     2461 ctcgccatga ccccaccagc ctcggcccag aacggcctcg accccgagaa cacgctcctc
     2521 atcgagctgg aggccggcac cgtgaccgtc gagctcctcc ccgacgtggc cccgcagcat
     2581 gtcgcccgcg tcaaggagct ggcgcgcgaa ggcttctatg acggcgtcgt ctttcaccgc
     2641 gtgatcgatg gcttcatggc gcagaccggc gatccgaccg gcacgggcac cggcggctcg
     2701 tcgaagcccg acctcaaggc ggaattctct aaggagccgt tcaagcgcgg gaccatcggt
     2761 gcggcccgca cccagaaccc gaacagcgcc aacagccagt tcttcatctg cttccagccc
     2821 gccccgcacc tcaacggcca gtacacggtc tggggccggg tggtcgacgg catggagctg
     2881 gtcgacg
//
LOCUS       DAOTFI010000387         2880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_41046_length_2880_cov_6.179469, whole genome shotgun sequence.
ACCESSION   DAOTFI010000387 DAOTFI010000000
VERSION     DAOTFI010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41046_length_2880_cov_6.179469"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1272)
                     /locus_tag="P5558_23490"
     CDS             complement(7..1272)
                     /locus_tag="P5558_23490"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY27348.1"
                     /translation="MTTRRALLGWMAGAALLATGGWATAVSAEQATLNALWMGWPEEQ
                     VDPLMDAFREAHPEITLNVERIPFAQIFQTIEVRLNARGPDPDIFIVDSPLTASYAVR
                     GYLLDLTPHLDASRFTKAGLAAGTVDGKIYSAPFGSSSALLFYNKKLLAEAGIEPPST
                     DPAERWTWAELVDAAKKVAKPDQGIWGFVFEQAERPYQLLPLPMSLGAEMISADGLTA
                     TGYLDSAKSVEAFQFYQDLYQKEQVSPPGVFDNNLTFELFGTGKVGFFVGNPYDLVNL
                     PNKYPDLEWGLAPHPYFADGTPVTPTGAWHIGVNPNTDQMEAALTFIDFMMQDDTQRL
                     WLSLRPYPPVLEAVWEAEADTTFADPGWGIVRHELAETARARPATPGWREYEDIMRLA
                     IRDIQTGADVQATLTAAAERIDRELAKYR"
     gene            1362..2309
                     /locus_tag="P5558_23495"
     CDS             1362..2309
                     /locus_tag="P5558_23495"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRY27349.1"
                     /translation="MTKLLIDCDPGHDDAVAILYAARHLDVVGITTVFGNASIENTTR
                     NALAICTLAGLDLPVAKGFGRPLVGPMTRFAPDGHGKTGIDGAELPEPAFEPVDQHAV
                     QFIIEQASAHRGELVLVVIGAQTNVGVALRLEPRLADWLAGISIMGGSALVGNLQPVQ
                     CVNVLSDPEAAHIVFSSGVPIHWVGYDMTRTVLIKEPDVERLRGGGRTARAVADLATF
                     YMGRQRAVLGVDGAPMHDSCAVIPFVRPELITYRDVPVEVELHGQLTRGMTVADLRTI
                     QPGEHQTIQPPKPANAKLAVDADRRGIIDAVVDAVLTYP"
     gene            complement(2487..2774)
                     /locus_tag="P5558_23500"
     CDS             complement(2487..2774)
                     /locus_tag="P5558_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRY27350.1"
                     /translation="MPMKDPPHPGEVIRELCIEPAGLTVTAAAEVLGVSRKALSELLN
                     GHSGISPEMAVRLSKAFGGSIESWLTQQMQYDLAKVSRRAAGIEVKRYEPV"
BASE COUNT          460 a          938 c          978 g          504 t
ORIGIN      
        1 cccgggtcag cggtacttgg cgagctcgcg gtcgatccgc tcggcggcgg cggtcagcgt
       61 cgcctggacg tcggcgccgg tctggatgtc gcgaatcgcg aggcgcatga tgtcctcgta
      121 ctcgcgccag ccgggcgtcg ccggccgggc tctggcggtc tcggccagct cgtggcggac
      181 gatgccccag cccggatcgg cgaaggtggt gtccgcttcg gcttcccaga ccgcctcgag
      241 caccggcgga tagggtcgca ggctgagcca gaggcgctgc gtatcgtcct gcatcatgaa
      301 gtcgatgaag gtcagggccg cctccatctg gtcggtgttc gggttgacgc cgatgtgcca
      361 ggcgccggtc ggcgtcaccg gcgttccatc cgcgaagtag ggatgcggcg ccagccccca
      421 ttcgagatcg ggatacttgt tcggcaggtt gaccaggtcg taggggttgc cgacaaagaa
      481 gccgaccttg ccggtgccga agagctcgaa ggtcaagtta ttgtcgaaga cgccgggcgg
      541 gctgacctgc tctttctggt agaggtcctg atagaactgg aaagcctcga ccgacttcgc
      601 cgaatcgaga tagccggttg ccgtcaggcc gtcggcgctg atcatctcgg cacccagcga
      661 catgggcagc ggcaagagct ggtagggtcg ctcggcctgc tcgaagacga agccccagat
      721 gccctggtcg ggcttggcga ctttcttggc cgcgtcgacc agctccgccc aggtccagcg
      781 ctcggccggg tcggtcgagg gcggctcgat gccggcctcc gccagcagct tcttgttgta
      841 gaacagaagc gcgctcgagc tgccgaaggg ggccgagtag atcttgccgt cgacggtgcc
      901 ggcagccagc ccggccttgg tgaaccggct ggcgtcgagg tgcggcgtca gatccagaag
      961 gtagccgcgc acggcgtagg atgcggtcag cgggctgtct acgatgaaaa tgtcggggtc
     1021 ggggcccctg gcgttgaggc ggacctcgat ggtctggaag atctgcgcga aggggatgcg
     1081 ctcgacgttc aaggtgatct ccggatgcgc ctcgcgaaag gcgtccatca agggatcgac
     1141 ctgctcctcc ggccaaccca tccagagcgc gttgagcgtc gcctgctcgg ccgagacggc
     1201 cgtggcccag ccgcccgtgg ccagcaaggc cgcgcccgcc atccagccga gaagtgctct
     1261 tcttgtggtc acgaccatgc ctccctatag catcggcatc actcaaacta cgcacctcga
     1321 tggaagtcaa ggtggcggcc cgggcctggg agacctagca attgacgaag ctcctcatcg
     1381 actgcgatcc cggtcacgac gacgcggtcg ccattctcta cgccgctcga cacctggacg
     1441 tggtcggcat caccaccgtc ttcggcaatg cctcgatcga gaacaccacc cgcaatgcgc
     1501 tcgccatttg caccctggcc gggctcgact tgccggtggc caagggcttt ggccgaccgc
     1561 tggtcggccc gatgacgcgt ttcgcccccg acgggcacgg caagaccggg atcgacggcg
     1621 ccgagctgcc cgagccggcc ttcgagcccg tcgatcagca tgccgtgcag ttcatcatcg
     1681 agcaggcgag cgcgcatcgg ggcgagttgg tgctggtggt gatcggggcg cagaccaatg
     1741 tcggcgtggc cctgcgcctg gagcccaggc tggccgactg gctcgccggc atctcgatca
     1801 tgggcggctc ggccctggtc ggcaatctgc agccggtgca gtgcgtcaac gtgctgagcg
     1861 accccgaggc ggcgcacatc gtcttctcca gcggcgtgcc gatccactgg gtcggctacg
     1921 acatgacccg gacggtgctg atcaaggagc ccgacgtcga gcgcctgcgc ggcggcggcc
     1981 ggacggcgcg tgccgtcgcc gacctcgcca cattctacat gggccggcag cgggcggtgc
     2041 tcggcgtcga cggtgcgccg atgcacgaca gttgcgccgt tatccccttc gtccgccccg
     2101 agctgatcac ctatcgcgat gtgccggtcg aggtcgagct gcacggccag ctcacgcgcg
     2161 gcatgaccgt ggccgacctg cgcaccatcc agccgggcga gcaccagacg atccagccgc
     2221 cgaagccggc gaatgcgaag ctcgccgtcg atgcggacag gcgcgggatc atcgacgcgg
     2281 tggtggatgc cgtcctgacc tatccctgac ggggcggcct caagccctga tttcgtgagg
     2341 aatcaaggaa ggagctgtcc ttccatgctg ccggcggtcc aagggagtcc tgcccctttt
     2401 ctttggaaag gttcctgacc ccatatgctc tcccacttgc cgggagggtg agatcagtca
     2461 tgtggactcg aacttcggac catggatcac accggttcat agcgtttgac ctcgatcccg
     2521 gcggccctcc tgctgacctt ggcgaggtcg tactgcatct gctgggtgag ccagctttcg
     2581 atgctgccgc cgaaggcctt ggacagcctg acggccattt ccggggagat cccggagtgg
     2641 ccgttgagca gctcggacag ggccttgcgg ctgacaccga gcacctcggc ggcggcggtg
     2701 acggtcagcc ctgccggctc gatgcacaac tcgcggatca cttcgccggg atgcgggggg
     2761 tctttcatcg gcatgttcga gatttcctag tgatagtcca ccagatcgac atcgtgtggc
     2821 tcctggtcct cgaagcggaa aacaatgcgc cagttgccgg acacgtccac ggcccagcag
//
LOCUS       DAOTFI010000388         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_41489_length_2857_cov_7.002141, whole genome shotgun sequence.
ACCESSION   DAOTFI010000388 DAOTFI010000000
VERSION     DAOTFI010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41489_length_2857_cov_7.002141"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="P5558_23505"
     CDS             complement(<1..379)
                     /locus_tag="P5558_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY27351.1"
                     /translation="MAKFGVGQPVRRVEDQRLVTGRGRYTDDISIDGQAHGYVVRSPH
                     AHARIRSIDTRAAAKAPGVLGVFTAKDFGPEGASVPCFIPITNKDGSERADPPHPILC
                     TDKVHYVGDNVAFVVAETLAQARD"
     gene            604..1344
                     /locus_tag="P5558_23510"
     CDS             604..1344
                     /locus_tag="P5558_23510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY27352.1"
                     /translation="MAVLELTGIAKHFGAIEALRGVDLTLEDGEVLGLMGDNGAGKST
                     LVKIIAGNFPPSAGQIRIDGKAMHFHKPVDAREAGIEVVYQDLALCDNLSAAANIFLG
                     REFKRKFGFFRFLDFGRMYQRAGELFEELQSETRPRDLVKQMSGGQRQAVAIARTRLS
                     EPRIVLMDEPTAAISVRQVAQVLDLIRRLRDQGIAIILISHRMPDVFAVCDHVAVLRR
                     GSLVADKSVADTSPEEVTGLITGAIARA"
     gene            1361..2389
                     /locus_tag="P5558_23515"
     CDS             1361..2389
                     /locus_tag="P5558_23515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27353.1"
                     /translation="MSTSVEISDVTRIREQSWFASLLANQALWVTVAVALICIATAIE
                     QPDSFATKNNFHNITRNFAAIGIIAMGMTAVIITGGIDLSVGSVMALSAIVAARLLES
                     GFDWYVGVAGGLMAGLVVGAINGALIAHIKLPPFVVTLGMLSIARSLAIVASDNRVIY
                     QFGEGGDTFKWLGGGRLDLPWIDGDVLPLSNMFVVLVVMAVIMSIVLRTTSWGRYLFA
                     IGGNEHAARLTGIPVDRIKIQVYMLAGFTAAIAGILSAGYGGSASNGMGKTYELYVIA
                     ATVIGGANLMGGKGSIYGAFIGAALIFLIRNSLIMFGVDANWYDLFVGLFIILAVLLE
                     RLRGRTRG"
     gene            2434..>2857
                     /locus_tag="P5558_23520"
     CDS             2434..>2857
                     /locus_tag="P5558_23520"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY27354.1"
                     /translation="MKKLLLGAVAAAALVAFGAPESQAQDKLVLALVPKNVNNPFFDQ
                     ARDGCKKAEAELGDIECLYIGPGEHGGGEEQVQVLSDLVARGVDGIAVSPSNAAAVVA
                     PLLEAKANGIPVLTWDSDLLPENKDLRIAYVGTKNYDIG"
BASE COUNT          465 a          964 c          945 g          483 t
ORIGIN      
        1 cgtccctggc ctgggcgagc gtctcggcga cgacgaaggc gacgttgtcg ccgacatagt
       61 ggaccttgtc ggtgcagagg atcgggtgcg ggggatcggc gcgctcgctg ccgtccttgt
      121 tggtgatcgg gatgaagcag ggcaccgaag caccttccgg gccgaagtcc ttggcggtga
      181 agacgccgag cacgcccggc gccttcgcgg cagcgcgcgt gtcgatcgag cggatccggg
      241 cgtgggcgtg cggcgagcgg accacgtagc cgtgcgcctg gccatcgatg ctgatgtcgt
      301 ccgtgtagcg accccggccg gtgaccagcc gctgatcctc gacccgtctg accggttggc
      361 caacgccgaa cttcgccatt ctgtcctccc aagctgcgct gcgccgcgcg atcaatgatg
      421 cctggcggga ttttaggccg cggcgacgcc agcacaaccg tgtcgtggcg aaaatcggcc
      481 gcatggccac gccgggccgt gcgtcagaac agggctttac cggtgccgca ggcccactat
      541 gctcgcctgc aacaaagacg acccgaccga tggcgcgagc caggcggcgg gacagggagg
      601 cacatggcgg tactcgagct cactggcatt gccaagcatt tcggggcgat cgaggccttg
      661 cgcggggtcg atctcacgct cgaggacggc gaggtgctcg gcctgatggg cgacaacggc
      721 gccgggaaat cgacgctggt caagatcatc gcgggcaatt tcccgccttc cgccgggcag
      781 atcaggatcg acggcaaggc catgcatttc cacaagcccg tggatgcgcg cgaggccggc
      841 atcgaggtcg tctaccagga cctcgcactc tgcgacaacc tgtcggccgc ggcgaacatc
      901 tttctcggcc gcgagttcaa gcgcaagttc ggcttctttc gctttctcga tttcggccgc
      961 atgtaccaga gggccggcga gctgttcgag gagctgcaat cggagacccg gccgcgcgat
     1021 ctggtcaagc agatgtccgg cggccagcgt caggcggtgg cgatcgcccg cacccggctg
     1081 tccgagccgc gcatcgtcct gatggacgag ccgaccgccg ccatcagcgt gcgccaggtc
     1141 gcccaggtgc tcgacctcat ccgccgcctg cgcgaccagg gcatcgccat catcctgatc
     1201 agccaccgca tgcccgacgt cttcgccgtg tgcgaccacg tcgccgtgct gcgccgcggc
     1261 agcctcgtgg ccgacaagtc ggtcgccgac acctcgccag aagaggtgac cggcctgatc
     1321 accggcgcca tcgccagggc ttgaaaggtc gaggaccagg atgagcacct ccgtcgagat
     1381 ctccgacgtc acccggatac gagagcagag ctggtttgcc tcgctcctcg ccaaccaggc
     1441 gctctgggtg accgtcgcgg tggcgctgat ctgcatcgcc accgcgatcg agcagccgga
     1501 ctcgttcgcg accaagaaca acttccacaa catcacccgc aacttcgccg ccatcggcat
     1561 catcgccatg ggcatgacgg cggtgatcat caccggcggc atcgaccttt ccgtcggctc
     1621 ggtgatggcg ctcagcgcca ttgtcgccgc acgcctgctc gaatcgggtt tcgactggta
     1681 tgtcggggtg gccggtgggc tgatggcggg cctcgtcgtc ggcgccatca acggggcgct
     1741 catcgcccac atcaagctgc cacccttcgt ggtgacgctc ggcatgctgt cgatcgcccg
     1801 ctcgctcgcc atcgtcgcgt ccgacaatcg ggtgatctac cagttcggcg agggcggcga
     1861 cacgttcaaa tggctcggtg gcggccgcct ggaccttccc tggatcgatg gcgacgtgct
     1921 gccgctctcc aacatgttcg tggtcctggt cgtcatggcg gtcatcatgt cgatcgtgct
     1981 caggacgacg tcctggggcc gctatctctt cgccatcggc ggcaacgagc acgccgcccg
     2041 gctgaccggc atccccgtcg accggatcaa gatccaggtc tacatgctgg ccggattcac
     2101 agccgccatc gccggcatcc tgtcggccgg ctacggcggc tcggcctcga acggcatggg
     2161 caagacctac gagctctatg tcatcgccgc cacggtgatc ggcggcgcca acctgatggg
     2221 cggcaagggc agcatctacg gcgccttcat cggtgccgcg ctgatcttcc tgattcgcaa
     2281 cagcctgatc atgttcgggg tcgatgccaa ctggtacgac ctcttcgtcg ggctcttcat
     2341 cattctcgcg gtgctgctcg agcggctccg cggcagaacg cgcgggtagg cccgcacgtg
     2401 gtcgaacttc gactgacaag ggaggtagag acagtgaaga agctactttt gggcgcggtc
     2461 gccgcggcgg cgctcgttgc gttcggcgca ccggagagcc aggcgcagga caagctggtc
     2521 ctggcgctgg tgccgaagaa cgtgaacaac ccgttcttcg atcaggcccg cgacggctgc
     2581 aagaaggccg aggccgagct cggcgacatc gagtgcctct atatcggccc gggcgagcac
     2641 ggcggcggcg aggagcaggt ccaggtgctc tccgacctcg tcgcccgcgg ggtggacggc
     2701 atcgccgtgt cgccctcgaa tgccgccgcc gtggtcgcac ccctgctcga ggccaaggcc
     2761 aacggcatcc cggtgctgac ctgggacagc gacctgctgc cggagaacaa ggacctgcgc
     2821 atcgcctatg tcggcaccaa gaactacgac atcggcg
//
LOCUS       DAOTFI010000389         2855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_41516_length_2855_cov_8.686429, whole genome shotgun sequence.
ACCESSION   DAOTFI010000389 DAOTFI010000000
VERSION     DAOTFI010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41516_length_2855_cov_8.686429"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2186)
                     /locus_tag="P5558_23525"
     CDS             complement(<1..2186)
                     /locus_tag="P5558_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY27355.1"
                     /translation="MNASDIAAEATVVRDPVCGMTVDPSAGKPRHEHTGHTYHFCSEG
                     CRAKFVAAPEQYIEATDPVCGMKVGRSTARHMTRHGGARFYFCSGRCKEKFEAEPGTY
                     LNGKPAPEPMPAGTIYTCPMHPEIEQIGPGDCPICGMALEPKGVPAGDEGPNPELVDF
                     RRRFAVGAVLTVPLLVLAMGPMLGLQVGDWIGERTARWLELVLATPVILWSGWPFLVR
                     GWKSFRTMNLNMFSLIAMGVAAAYLFSVVAVIAPGLFPHGFQDHSGQVGVYFEAGAVI
                     VVLVLLGQILELQARERTGSAIRALLDLAAKTARLIRPDGREEEVPLEEVQVGDRLRV
                     RPGEKVPVDGEVVEGRSSIDESMLTGEPVPVEKVAGDPVTGATINGTGSLIIEARRVG
                     KDTMLAQIVGMVAAAQRSRAPIQKLADAVAGWFVPAVIGVAVLAFVAWAIWGPTPALA
                     YALISAIAVLIIACPCALGLATPVSIMTATGRGAQAGVLIKNAEALETFAKVDTLIVD
                     KTGTLTVGKPELVAVLPGPGHDEADVLRLAASLERGSEHPLAEAIVRGAEARGIDLTA
                     AEDFEAVTGKGVKGIVDGKTVVLGNATMLADMGLDRTASDEADARRDQGETVMFVVVD
                     GAFAGLVSVADPVKETTPAALKALHELGFRIVMATGDSERTAQAVAGKLGIDEIRAEV
                     LPEDKARIIRELQAEGAKVAMAGDGVNDAPALAQADVGIAMGTGADV"
     gene            2310..2720
                     /gene="cueR"
                     /locus_tag="P5558_23530"
     CDS             2310..2720
                     /gene="cueR"
                     /locus_tag="P5558_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HRY27356.1"
                     /translation="MNIGDVAERSGLPAKTIRYYEDIGLIRPKRSGNGYRFFGSVDLH
                     KLAFLGRARSLGFTIEECRALLALYEDEGRASSEVKQVAEGHLEQIDRKIAELQAMRA
                     TLAKLIHRCHGDDRPDCPILDDLAGEDGATTVHG"
BASE COUNT          456 a         1019 c          954 g          426 t
ORIGIN      
        1 acgtcggcgc cggtgcccat ggcgatgccg acatcggcct gcgccagcgc cggcgcgtcg
       61 ttgacgccgt cgccggccat ggcgaccttg gcgccctcgg cctgcagctc ccggatgatg
      121 cgcgccttgt cctcgggcag aacctcggcg cggatctcgt cgatgccgag cttcccggcc
      181 accgcttgtg ctgtgcgctc gctgtctccg gtggccatga cgatgcggaa accgagctcg
      241 tgcagcgcct tcagcgccgc cggcgtcgtc tccttcaccg gatcggcgac gctgacaagc
      301 ccggcgaagg ccccgtcgac caccacgaac atcacggtct cgccctggtc ccggcgggcg
      361 tcggcttcgt cggacgcggt gcgatcgagg cccatgtcgg ccagcatcgt cgcgttgccg
      421 agcaccacgg tcttgccgtc gactatgccc ttcacccctt tgccggtcac cgcctcgaag
      481 tcctcagcgg cggtgaggtc aataccccgc gcctcggcgc cgcgcacgat cgcctcggcc
      541 agcggatgct cggagccgcg ctcgagcgag gcggcgagcc gaagcacgtc cgcctcgtcg
      601 tggccaggcc cgggcagcac ggcgacgagc tcgggcttgc cgacggtgag cgtcccggtc
      661 ttgtcgacga tgagcgtgtc gaccttggcg aaggtctcga gcgcttccgc gttcttgatc
      721 aggacgcccg cctgcgcgcc gcgccccgtg gcggtcatga tcgagaccgg cgtcgcgaga
      781 ccgagcgcgc aggggcaggc gatgatcagc accgcgatcg ccgagatcag cgcataggcg
      841 agcgccggcg tcggtcccca gatcgcccag gcgacgaagg ccagcaccgc gaccccgatc
      901 accgccggca cgaaccagcc ggcgacggca tccgcgagct tctggatcgg cgcgcgcgat
      961 cgctgcgccg ccgccaccat cccgacgatc tgcgcgagca tggtgtcctt gccgacgcgc
     1021 ctggcttcga tgataaggct gccggtgccg ttgatggtgg cccccgtcac cgggtcgccg
     1081 gcgaccttct cgaccggaac cggctcgccg gtcagcatcg actcgtcaat cgacgagcgg
     1141 ccctcgacca cctcgccgtc gaccggcacc ttctcgcccg ggcgcacccg cagccggtcg
     1201 ccgacctgca cctcctcgag cggaacttcc tcctcgcgac cgtcaggacg gatcagtcgt
     1261 gcggtcttgg cggctaggtc gagcagcgcc cggatcgccg agccggtccg ctcgcgcgcc
     1321 tgcagctcga ggatctggcc gagcagcacc agcacgacga tgacggcgcc ggcctcaaag
     1381 tacacgccga cctggccgga atgatcctga aagccatgcg ggaacaggcc cggcgcgatc
     1441 accgcaacga cgctgaagag ataggccgcg gcgaccccca tggcgatcag gctgaacatg
     1501 ttgaggttca tcgtccggaa cgacttccag ccgcggacga ggaatggcca gcccgaccag
     1561 aggatcaccg gcgtcgccag gaccagctcc agccagcggg cggtccgctc cccgatccag
     1621 tcgcccacct gcaggccgag catcggaccc atcgcgagga cgagcagcgg cacggtcagg
     1681 accgcgccga ccgcgaagcg gcggcggaag tcgacgagct cgggattggg cccctcgtca
     1741 ccggccggca cgcctttcgg ctccagcgcc atgccgcaga tcggacaatc gccggggccg
     1801 atctgctcga tctccggatg catcgggcag gtatagatgg tcccggccgg catcggctcc
     1861 ggagcgggct tgccattgag ataggtcccg ggctccgcct cgaacttctc cttgcagcgg
     1921 ccggagcaga aatagaaccg cgcgccgcca tgcctggtca tgtgcctggc cgtcgagcgg
     1981 ccgaccttca tgccgcagac cggatcggtt gcctcgatgt attgctcggg tgccgcgacg
     2041 aacttcgcac ggcacccctc cgagcagaag tggtaggtgt gaccggtgtg ttcgtggcgc
     2101 ggtttgcccg ccgaagggtc gacggtcatc ccgcagaccg gatcgcggac caccgtcgcc
     2161 tctgccgcga tgtcggatgc gttcatgtca gcctcccgat tctggatccg acaccccata
     2221 tgcaccttcc agcaactgga gggtcaaggg gtgcttgacc ctccagccgc tgaaagcgct
     2281 acgatgggaa ccaacggagg ggatcgacca tgaacatcgg agacgtcgca gagcgaagcg
     2341 ggttgccggc gaagaccatc cgctactacg aggacatcgg tctgatccgg ccgaagcgca
     2401 gcggcaacgg ctaccgcttc ttcgggagcg tcgacctgca caagctcgcc ttcctcggcc
     2461 gcgcgcggtc gctcggcttc acgatcgagg agtgccgggc gcttctggcg ctctacgagg
     2521 acgagggccg tgccagcagc gaagtgaagc aggttgcaga agggcatctg gagcagatcg
     2581 accgcaagat cgcggagctc caggcgatgc gcgcgaccct ggcgaagctg atccatcgct
     2641 gccacggcga tgatcgtccc gactgcccga tcctcgacga cctggccgga gaggacggcg
     2701 cgacgaccgt ccatggatag cggcaagcga gcggttgcta cgctgatcgt cgcggccatc
     2761 gacgcacggg ccggcgctcg cgtcgcatgc atccgctgaa gcgccggtgg cggtcggcgc
     2821 gcgggtttat gccgagcaat gcgcctcctg ccacg
//
LOCUS       DAOTFI010000390         2848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_41646_length_2848_cov_5.378446, whole genome shotgun sequence.
ACCESSION   DAOTFI010000390 DAOTFI010000000
VERSION     DAOTFI010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41646_length_2848_cov_5.378446"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..495
                     /locus_tag="P5558_23535"
     CDS             31..495
                     /locus_tag="P5558_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY27357.1"
                     /translation="MRRAPLAIAVVLVGASAAGVAWLSFGQGGSSGPTLRQLENGEEI
                     YAANCAACHGVNLEGEPGWQSRLPSDRLPAPPHDASGHTWHHPDGVLFRITKEGPAAV
                     VGGNYESDMPSFGDVLSDDEIWAVLTFIKSRWPERERQLQAEMSRKEKEADQ"
     gene            492..1037
                     /locus_tag="P5558_23540"
     CDS             492..1037
                     /locus_tag="P5558_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY27358.1"
                     /translation="MKPLLSLFLLLVALKGPAVGATESGPMPLHEKPPPVPAIAFTDE
                     AGNALTLEDWRGRVVLLNIWATWCAPCRAEMPTLDRLQATVGSEQFEVLALSIDRAGV
                     GAVRTFFDEIGIAHLRLFIDETMRTSRDLGILGLPATLLIGPDGRELGRLLGPAEWDA
                     PEMIAFLERVIADTTGKEQEP"
     gene            1034..1357
                     /locus_tag="P5558_23545"
     CDS             1034..1357
                     /locus_tag="P5558_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27359.1"
                     /translation="MTTRDLAPSTATWRPAGTTVPPWLLQPRNLALGGVAAIGAGLAL
                     NWGWLTAVGAAPLLLGVLPCLGMCALGLCMRGRPAGTTAAANAAEPEIPASQDSSAAP
                     PVSPP"
     gene            1408..1725
                     /locus_tag="P5558_23550"
     CDS             1408..1725
                     /locus_tag="P5558_23550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27360.1"
                     /translation="MRNTFKLLVVGLGLLATGATAQETATPDTGSPMPGTASGQGMMG
                     QGDMPGMMGQGGMAGQGGMMGMMEMMQQMAPMMQQCSEMMAAMANQMPPAPSAPSSPS
                     DNG"
     gene            1784..2470
                     /locus_tag="P5558_23555"
     CDS             1784..2470
                     /locus_tag="P5558_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRY27361.1"
                     /translation="MRSILLAVLLGAIAAFAATARAHHPGADLDKMMGDKERFFQVID
                     KPAPPFELADADGNIVRLADFADKIVVLNFVFAGCTDVCPLHSALIAAVQSKVNVTPM
                     RDLVQFISVTTDTAVDTPDMLRAYGDQHGLDPQNWLFLTSRPGDTETGTRDLARRYDL
                     VFEPLDDGQQMHGVVTHVIDRDGRLAARFHGLRFDQLSLVLYLDGLVNAPPDQPAPAG
                     WWQGLEGLFR"
     gene            2467..>2848
                     /locus_tag="P5558_23560"
     CDS             2467..>2848
                     /locus_tag="P5558_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27362.1"
                     /translation="MTPAETRAESPDASSSSVWLPTLRRYMTFVAMANLLWEFAHLPL
                     YTLWETGTRSELLFAAVHCTGGDILIALGTLVLALVLVGNPAWPAAGMARVITLTVAL
                     GLAYTVFSEWLNIEVREAWAYRDLM"
BASE COUNT          463 a         1009 c          929 g          447 t
ORIGIN      
        1 cgagcacgag gtcttcggcg gcaggcgctg atgcggcgag cccccctcgc catcgcggtc
       61 gtgctagtcg gtgcttccgc ggccggcgtg gcttggctca gcttcggcca gggcggctcg
      121 tcggggccga ccttgcggca actcgagaac ggcgaagaga tctacgccgc gaactgcgcc
      181 gcctgccatg gcgtgaatct cgagggcgaa cccggctggc agagccggct gccgtcggac
      241 cgcctgccgg cgccgccgca cgacgcgtcc gggcatactt ggcaccaccc cgacggcgtt
      301 ctcttccgca tcaccaagga agggccggcg gcggtcgtcg gcggcaacta cgagagcgac
      361 atgcccagct ttggtgacgt gctttccgat gacgagatct gggctgtgct cacttttatc
      421 aagagccggt ggccggaacg cgagcgccaa ctacaggccg agatgagccg aaaagagaag
      481 gaggccgatc aatgaagcca ctactgagtc tcttcctgct gctcgtcgcc ctcaaggggc
      541 cggccgtcgg cgccaccgag tcgggtccca tgccgctgca cgagaagcca ccgccggtgc
      601 ccgccatcgc gtttactgac gaagccggca acgcgctgac gctcgaggac tggcgcggca
      661 gggtcgtgct cctcaacatc tgggccacct ggtgcgctcc ctgtcgtgcc gagatgccga
      721 cactggatcg gctccaggcg acggtcggca gcgagcaatt cgaggttcta gcgctgtcga
      781 tcgaccgtgc aggtgttggc gccgtgcgga ccttcttcga cgagatcggc atcgcgcacc
      841 tccgcctgtt catcgacgag acgatgagga cgtcgcggga tttgggaatt cttggtctgc
      901 ccgcaaccct gctcatcggc ccggacggcc gagaactcgg tcgactgctc ggaccggccg
      961 aatgggacgc gcctgagatg atcgccttcc tcgagagggt gatcgccgac accactggaa
     1021 aggagcaaga accatgacca cgagagacct cgcgccgtcg acggcaacgt ggcggcccgc
     1081 aggaacgacc gttccgccgt ggctgctgca gccgcgcaac ctggcgcttg gcggcgtcgc
     1141 cgccatcggc gccggcctcg ccctcaactg gggctggctg accgcggtag gcgctgcacc
     1201 gctcctgctc ggcgtgctgc cgtgcctcgg catgtgcgcg ctcgggctct gcatgcgcgg
     1261 caggccggcc ggcactactg cggcggccaa cgccgccgag cccgaaattc cggccagcca
     1321 ggactccagc gccgcgccac ccgtctcgcc gccatgagcc gatccgcaac cacgaacctc
     1381 cccgacccca accaacggag catagccatg cgcaacacgt tcaagcttct cgtcgtcggc
     1441 ctcggcctcc tggccaccgg cgccacggcc caggagaccg cgacacccga caccggcagc
     1501 ccgatgcccg gcacggcatc cggccagggc atgatgggcc agggcgacat gcccgggatg
     1561 atgggccagg gcggcatggc gggccagggc ggcatgatgg gcatgatgga gatgatgcag
     1621 cagatggcgc cgatgatgca gcagtgcagc gagatgatgg ccgccatggc gaaccagatg
     1681 ccgcccgccc cgtcggcacc gagctcccct agcgacaacg gctgagcgag ctgcccgccc
     1741 tgccgccctt ggcggcaggg cgccttttgg aggagacgtc gtcatgcgct cgatacttct
     1801 cgcggtcctg ctgggcgcaa tcgccgcctt cgcggccacc gccagggccc accacccggg
     1861 agccgacctc gacaagatga tgggcgacaa ggagcgcttc ttccaggtca tcgataagcc
     1921 ggcgccgccg ttcgagctgg cggacgccga tggcaacatc gtccgcctcg ccgactttgc
     1981 cgacaagatc gtcgtcctga atttcgtctt cgccggctgc acggatgtct gcccgctgca
     2041 ctccgccctg atcgcggctg tccagtctaa ggtcaacgtc acgccgatgc gggacctcgt
     2101 ccagttcatc agcgtgacca ccgacaccgc ggtcgacacc cccgacatgc tgcgagccta
     2161 cggcgaccaa cacgggctcg atccgcagaa ctggctgttc ctgaccagcc gcccgggcga
     2221 cactgagacc gggactcgcg acctcgcccg gcgctacgac ctcgtgttcg agccactcga
     2281 cgacggccag cagatgcatg gcgtggtgac ccacgtgatc gaccgggacg ggcgcctcgc
     2341 ggccaggttc cacggcctgc gcttcgatca gttgagcctc gttctctacc tcgacgggct
     2401 ggtcaacgcg ccacccgacc agccggcgcc ggccggctgg tggcagggcc tcgagggcct
     2461 gttccgatga cacctgccga aacacgagcc gaatcgcccg atgcatccag ttccagcgtg
     2521 tggctgccga cgttgcgccg atacatgacc ttcgtggcga tggcgaacct gctttgggag
     2581 ttcgcccacc tgccgctcta tacgctctgg gaaaccggca ccaggtccga gctgctgttc
     2641 gccgccgtgc actgcaccgg cggtgacatc ctgatcgcgc tcggcacgct cgtgctggct
     2701 ttggttctgg tcggcaaccc ggcctggcct gcagcgggca tggcgcgcgt catcacactg
     2761 accgtggccc tcggactggc ctacacggtg ttcagcgaat ggctcaacat cgaggtccgc
     2821 gaggcatggg cctatcgcga cctgatgc
//
LOCUS       DAOTFI010000391         2823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_42167_length_2823_cov_7.869581, whole genome shotgun sequence.
ACCESSION   DAOTFI010000391 DAOTFI010000000
VERSION     DAOTFI010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42167_length_2823_cov_7.869581"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="P5558_23565"
     CDS             complement(<1..569)
                     /locus_tag="P5558_23565"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRY27363.1"
                     /translation="MRRRSSSIRVRRPLPMLPPCSALARRGSRLAGCLVLATALLGSV
                     SPTALAAGRHALLIGNSAYSALPPLDNPSLDARLLAKTLGELGFAVSAVTDADYDGMR
                     AAIDAFRREARDAEVALVYYAGHGVQVDGVNYLLPVDAVVQGRDDLRRQGLVLALLLH
                     EIEALAPEFGILVLDACRNNPVAELEAELA"
     gene            complement(569..1135)
                     /locus_tag="P5558_23570"
     CDS             complement(569..1135)
                     /locus_tag="P5558_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27364.1"
                     /translation="MKIERAAGLALAILALGEPAMAQDVSGLVAYRSSNSSGSTEAAR
                     VIADMIAERSQSQAVRERAQALRPTGAFSVGPDELLADIGAYRFIDQLPAVQPVAAAP
                     SVAPIRTSRPAPAPAPAPVATPAPAPAPAPAPAVAPTPPPAATAVVPVVEERQRSQGR
                     NWGVGPTATTDQGGSNSGGGGGGGGGWN"
     gene            complement(1153..1641)
                     /locus_tag="P5558_23575"
     CDS             complement(1153..1641)
                     /locus_tag="P5558_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27365.1"
                     /translation="MMKRKSRREDPRLGFGRGVNGMLDRPRRAALVAVLLLSACSDGS
                     ISSLDPANLFNSSGASQTTAVDPGSALVEARRCGRALAVAARCNYVRDDRDFAALRFT
                     VLQGLDQRYGAVIDGPRLTEAVDLAVLDRMSTIGQCSIAPSQVAVVESGLRDVLGTCA
                     AP"
     gene            complement(1702..2217)
                     /locus_tag="P5558_23580"
     CDS             complement(1702..2217)
                     /locus_tag="P5558_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27366.1"
                     /translation="MRRLQPDRRTSALRPALLAGFAALLLAMPARAEVVLAPGQAASS
                     SGAASGSGSSTCPGVKVGGRLTNTVAIDQLESSASGRNARTVVETNRITNTTVGGDLT
                     ATTEVGQLTQRAEGRDATAITVIGTIEDVSTGGDLTSIVALGTSLNVAIGDGAWACVE
                     LGTLGGGPVCR"
BASE COUNT          444 a          997 c          988 g          394 t
ORIGIN      
        1 gccagctccg cctcgagctc ggccaccggg ttgttgcggc aggcatcgag gacgaggatg
       61 ccgaattccg gggcgagcgc ctcgatctcg tgcagaagca gggccaggac cagcccctgc
      121 cggcgcaggt cgtcccgccc ctgcaccacg gcgtcgaccg gcaggagata gttcaccccg
      181 tcgacctgca cgccgtgccc ggcataatag accagcgcca cctcggcatc gcgcgcctcg
      241 cgccggaagg cgtcgatggc cgcacgcatg ccgtcatagt cggcgtcggt cacggcgctg
      301 acagcgaagc cgagctcgcc cagcgtcttc gccagaagcc tggcatcgag cgacgggttg
      361 tcgagcggcg gcagggccga gtaggccgag ttgccgatca ggagggcgtg cctgccggcg
      421 gcgagtgcgg tcggcgagac gctgccgagc agggccgtgg cgagaaccag gcagccggcc
      481 aggcggctac cccgtcgcgc caacgcgctg catggcggca acatcggcaa gggccggcga
      541 acccggatcg acgagctgcg gcggcgcatc agttccagcc tcctccgcca ccgccaccgc
      601 cgccgctgtt gctgccgccc tggtcggtgg tcgccgtggg gccgacgccc cagttgcgcc
      661 cctgcgaccg ctgccgctct tcgaccaccg gcaccacggc cgtcgcggcc ggcggcgggg
      721 tgggtgccac ggctggcgcg ggagcaggcg ccggggctgg tgcgggagtc gccacgggag
      781 ccggggcggg agcgggagcc ggccgggacg tgcggatcgg tgccacgctc ggcgcggccg
      841 ccaccggctg gacggccggc agttggtcga tgaagcgata ggcgccgata tccgcgagca
      901 gctcgtccgg gccgacgctg aaggcgccag tcggccgcag ggcctgcgcc cgctcgcgca
      961 ccgcctggga ctggctgcgc tcggcgatca tgtccgcgat caccctggcc gcctcggtgc
     1021 tgccgctgct gttgctgctg cggtaggcca ccagcccgct cacatcctgg gccatggccg
     1081 gctcgcccag ggccaggatg gccagggcaa gtcccgctgc tcgctcgatt ttcatgacat
     1141 gaagcctttc cttcatggcg cggcacacgt gccgagcaca tcgcgcaggc cactctcgac
     1201 cacggccacc tgcgatggtg cgatcgagca ctgaccgatg gtcgacatcc ggtcgagcac
     1261 cgccagatcc acggcctccg tcagtcgcgg gccgtcgatc acggcgccat agcgctggtc
     1321 caggccctgc agcacggtga agcgcagggc cgcgaaatca cgatcgtcgc gcacatagtt
     1381 gcagcgcgcg gccaccgcca gcgcccgtcc gcaacgacgt gcctcgacca gggcgctgcc
     1441 cggatcgacg gcggtcgtct ggctcgcccc ggacgaattg aagaggttcg ccggatccag
     1501 ggaggagatg gagccgtcgc tgcaggcgga cagaagaagg accgcgacaa gggcggcccg
     1561 acggggtcgg tcgagcattc catttactcc acggccaaag ccgagcctcg ggtcttctcg
     1621 cctcgacttg cgcttcatca tagctgatct catggcattc gcccattgtc gctgggtgat
     1681 caccgcggcc gtcgcaaggc gtcagcggca gacggggccg ccacccaggg tcccaagctc
     1741 gacgcaggcc caggcgccgt cgccgatcgc cacgttgaga ctggtgccca gggccacgat
     1801 gctggtcagg tcgccgccgg tgctgacgtc ctcgatggtg ccgatgacgg tgatggccgt
     1861 ggcatcgcgg ccctcggcgc gctgcgtcaa ctggccgacc tccgtcgtcg ccgtgagatc
     1921 gccacccacc gtggtgttgg tgatccggtt ggtttcgacg accgttcggg cattcctgcc
     1981 gctggcggag ctctcgagct ggtcgatggc gacggtgttg gtgagcctgc cgccgacctt
     2041 gacgccgggg caagtggagg acccggagcc cgacgcggca ccggatgagg acgccgcctg
     2101 gcccggcgcg agcaccacct cggcccgggc cggcatggca agcaagaggg ccgcaaagcc
     2161 ggcgagcagc gccgggcgca gggcggaggt gcggcggtcg ggctggaggc ggcgcatcct
     2221 gggtctcctc gaagggcgcc aaggaccgtt ccttgccgcc gaaccgagac tgcccggccg
     2281 acggcaccgc gccgagggtg gaaaaaacct acccttccgg ctacccgagc accgcaccgc
     2341 cgaaccggcg cctaacgcga gatgctttca cattgtcgtg atcgatcgcg gccgcagcct
     2401 ggctacggtc ggaaccggcc cgctccttgt cctgccggcg ggggcgatcg gcgacccttg
     2461 gaaaaaccct gccttacggt gatggggatc gctgacagtt tcggggtcac cgaacggcaa
     2521 gacccgacaa aatgtgagtt ctgccaccga cgagggtctg accgggcggc acgatcgacc
     2581 ccggcaggaa tgcggccaat gcattggtca cctcgtcggg caagtgataa aacccggtga
     2641 ggcctgcgac agactaacgc ggtttcaccc ttacccacag gagaccgacg atgaagacct
     2701 cgaagctcac cgcagtggcc ctcggcatct cgctccttac cgccaccgcc ctctcgacgg
     2761 gtgcgctcgc ccagcagggc ggcgtgcaga tcggcggcaa cgccaacaac acgaccctgc
     2821 tca
//
LOCUS       DAOTFI010000392         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_42536_length_2804_cov_6.032739, whole genome shotgun sequence.
ACCESSION   DAOTFI010000392 DAOTFI010000000
VERSION     DAOTFI010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42536_length_2804_cov_6.032739"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1797)
                     /gene="ade"
                     /locus_tag="P5558_23585"
     CDS             complement(112..1797)
                     /gene="ade"
                     /locus_tag="P5558_23585"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRY27367.1"
                     /translation="MSVLQQNLAARIDQALAREPADLVIRGACLLNTATGRIEGPMDI
                     AIAGDTIVGTHGVYEGQHEIDAKGRVVAPGFIDTHVHVESSMVLPAEFERGVLPRGTT
                     TAICDPHEIANVIGLDGITYFIEASRQLAMTLKVNLSSCVPATELETAGARLEADDLL
                     PLLADPAVIGLAELMNFPGVIHKDAGMLAKLERFHNQHIDGHSPLLRGSELNAYLATG
                     VLTEHECTSLEEAEEKLAKGMHILLREGSIAKNVTTLAPVITDATWMRCAFCTDDRNP
                     LEIREEGHIDHAIRLAIRAGAPAIPAYRAATLGAALAFGLRDRGVIAPGKRADLVILD
                     DLEEVRVAEVVVGGRPAADALAEPRPHPAAPGYNSVRREPVAATDLALDGDTRTHVIG
                     ALPFSLLTDHLEATPAEDPEIARLCVLERHGLNGNIGKGWVKGFGRVRGAIATSIGHD
                     SHNLITVGDDPADMARATNHLIELQGGAVVVRDGQVLADLPLPIAGLMSDQNLAFVDD
                     RLIELRAAARNIGCTLQEPLLQLAFLPLPVIPHLKLTDRGYVVAGPEGLRLLD"
     gene            complement(1841..2509)
                     /gene="msrA"
                     /locus_tag="P5558_23590"
     CDS             complement(1841..2509)
                     /gene="msrA"
                     /locus_tag="P5558_23590"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRY27368.1"
                     /translation="MWLLKKKSADLPTADQALPGRAARAYAVPARHAVLGHELEPPYP
                     AGIEVAHFGMGCFWGAERRFWQQEGVWTTAAGYAAGTTPNPTYEEVCSGLTGHNEVVL
                     VAWDPAVISFEALLKVFFESHDPTQGMRQGNDVGTQYRSGIYTTTEAQLAAAKATATA
                     YGRKLAAAGMGRVTTEIAPAGEFWFAEGYHQQYLAKNPMGYCGLRGTGVACAIDTAET
                     MTPA"
     gene            complement(2584..>2804)
                     /locus_tag="P5558_23595"
     CDS             complement(2584..>2804)
                     /locus_tag="P5558_23595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27369.1"
                     /translation="GDVARAIAELGTASDALLRVLAAPAADRPGAIAGLATTLPAFLR
                     RLERHLDDEEDLVIPLLIDRGEGPLGIG"
BASE COUNT          383 a          836 c         1135 g          450 t
ORIGIN      
        1 cggcaatggt cgcaaaaagt gtcctgaccc cattaggaat tcgttcttgt ttccttgcgc
       61 ggtattggtc acgccacctg ctgccgcgcc gggcaaccga cccgggatag gctagtccag
      121 caaccgcagc ccctccggcc ccgccacgac atagccccgg tcggtcagct tgaggtgcgg
      181 gatcaccggg agcggcagga acgctagctg gagcagcggc tcctggagcg tgcagccgat
      241 gttgcgggcg gctgcgcgga gctcgatcag gcgatcgtcg acgaaggcga ggttctggtc
      301 ggacatgagg ccggcgatgg ggagcgggag gtcggcgagg acctggccgt cccggacgac
      361 cacggcacca ccctggagct cgatcaggtg gttcgtggcc ctggccatgt cggcgggatc
      421 gtcgccgacg gtgatcaggt tgtggctgtc gtggccgatg gaggtggcga tggcaccgcg
      481 gaccctgccg aagcccttga cccagccctt gccgatattg ccgttcagcc cgtggcgctc
      541 gaggacgcag aggcgggcga tctccgggtc ctcggccggc gtggcctcga ggtggtcggt
      601 caggagggag aaggggagcg cgccgatgac gtgggttctg gtgtcgccgt cgagggcgag
      661 gtcggtggcg gcgacgggct cgcggcggac gctgttgtag ccgggggcgg cggggtgggg
      721 gcggggctcg gcgagtgcgt cggctgccgg ccggccgccg accacgacct cggcgacgcg
      781 cacctcctcc agatcgtcga ggatgacgag gtcggcgcgc ttgccgggtg cgatcacgcc
      841 acggtcgcgc aggccgaagg cgagggcggc gcccagcgtg gcggcgcggt aggcggggat
      901 ggcgggggca ccggcgcgaa tcgcgaggcg gatggcgtgg tcgatatggc cttcctcgcg
      961 gatctccagc gggttgcggt cgtcggtgca gaaggcgcag cgcatccagg tggcgtcggt
     1021 gatgacgggg gcgagggtgg tgacgttctt cgcgatggag ccttcgcgca ggaggatgtg
     1081 catgcccttg gcgagctttt cctcggcctc ctcgaggctg gtgcattcat gctcggtgag
     1141 gacgcccgtg gcgagatagg cgttgagctc gctgccgcgc aggagcgggc tgtggccgtc
     1201 gatgtgctgg ttgtggaagc gctccagctt ggccagcatg ccggcgtcct tgtggatgac
     1261 gcccgggaag ttcatgagct cggcgaggcc gatgacggcg gggtcggcca agagcggcag
     1321 gagatcgtcg gcctccagcc tggcgccggc ggtctcgagc tcggtggcgg ggacgcagga
     1381 gctgagattg accttcagcg tcatggcgag ctggcggctc gcctcgatga agtaggtgat
     1441 gccgtcgagg ccgatgacgt tggcgatctc gtgcgggtcg cagatcgccg tggtggtgcc
     1501 gcggggcagc acgccgcgct cgaactcggc cggcaggacc atggagctct cgacgtggac
     1561 gtgggtgtcg atgaagccgg gcgccacgac gcggcctttg gcgtcgatct cgtgctggcc
     1621 ctcgtagacg ccgtgcgtgc cgacgatggt gtcgccggcg atggcgatgt ccatcgggcc
     1681 ctcgatccgg ccggtggcgg tgttgagcag gcaggcgccg cggatgacga ggtcggcggg
     1741 ctctctcgcc agcgcctggt cgatgcgggc ggcgaggttc tgctgcagga cggacatgct
     1801 ttgctccggt gcgctggcac cggagcgtca tatcaggcga tcaggcgggc gtcatggtct
     1861 ccgctgtgtc gatggcgcag gcgacgccgg tgccgcggag gccgcaatag cccatcgggt
     1921 tcttcgcgag gtattgctgg tgatagccct cggcgaacca gaactcgcct gcgggggcga
     1981 tctcggtcgt gaccctgccc atgccggcgg cggcgagctt cctgccgtag gcagtggcgg
     2041 tggccttcgc ggctgcgagc tgggcctcgg tggtcgtgta gatgccggag cggtactgcg
     2101 tgccgacatc gttgccctgg cgcatgccct gggtcgggtc gtggctctcg aagaagacct
     2161 tgaggagggc ctcgaagctg atgacggcgg gatcccaggc gaccagcacc acctcgttgt
     2221 ggccggtcag gccggagcag acctcctcgt aggtcgggtt gggggtcgtg cccgcggcat
     2281 agccggcagc ggtcgtccag accccttcct gctgccagaa gcggcgctcc gcaccccaga
     2341 agcagcccat gccgaagtgg gccacctcga tgccggccgg gtagggcggc tcgagctcgt
     2401 ggccgagcac ggcatggcgc gcgggcacgg cataggcgcg ggcggcgcgg ccggggaggg
     2461 cctggtcggc cgtcggcagg tcggcggatt tcttcttcag gagccacatg ggcggggcct
     2521 cgcaggggag ggaagcgttg aacatggaaa tggcgcgcaa ggcggccgcg taaaggggtg
     2581 gaatcagccg atgccgagcg gtccttcgcc ccggtcgatg aggagcggga tgacgaggtc
     2641 ctcctcgtcg tcgagatgac gctcgaggcg gcgcaggaaa gcggggaggg tggtggcgag
     2701 cccggcgatg gcgcccgggc gatcggcagc gggtgctgcc aggacgcgga gcagggcgtc
     2761 gctggcggtg ccgagctcgg cgatcgcccg ggcgacatcg ccgg
//
LOCUS       DAOTFI010000393         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_42640_length_2799_cov_7.245991, whole genome shotgun sequence.
ACCESSION   DAOTFI010000393 DAOTFI010000000
VERSION     DAOTFI010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42640_length_2799_cov_7.245991"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..516)
                     /locus_tag="P5558_23600"
     CDS             complement(109..516)
                     /locus_tag="P5558_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27370.1"
                     /translation="MRHLLLIALLLALTGPALAKVKKIFKTIGNDIYGDPLYAQDFMQ
                     PFQSHGVHEVDDVGIVIRGKFMARPGRQFVLRKEIYDRVQRAFEQHGIQFARREIRVR
                     IDGTSEQHAQAAGAAAANAVPAEAQTAGTSDDR"
     gene            complement(527..1894)
                     /gene="mgtE"
                     /locus_tag="P5558_23605"
     CDS             complement(527..1894)
                     /gene="mgtE"
                     /locus_tag="P5558_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRY27371.1"
                     /translation="MTETEAPPRSDEDMLDEALERDDGPALEALLAEQPPSEALRLTL
                     ELSVEDRNRMLGLVSAEAAARIVEEAPAELAAEIVEELDAGVAASIVEELDSDDQVDL
                     IDELDTADAEAILARMDPADAADVRRLIQYDSDTAGGLMVAETFAFRVDETVGAVLRG
                     LTVEDEDFERYRGQHPYVVDAEGRLQGVVSLRTLLTIRRGTVLRDVMAKAISVPPTAS
                     IDELENLFDEHPFLGIPVTDARGVLIGVVSRIAIAEAVQERTESDSLKMQGVIGDELR
                     SMPTFVRARRRLAWLSANIVLNIIAASVISAYEETLAAVIAIAVFLPMVSDMSGCSGN
                     QAVAVSLRELALGLTRPTDIARVWLKEISVGVMNGLVLGLLIGVVCWVWKGNPWLGVV
                     IGTALAINTMIAVSIGGIVPLVLKRFGTDPAVASGPLLTTVTDMAGFFLVLSLATMMM
                     PLLVK"
     gene            2095..>2799
                     /locus_tag="P5558_23610"
     CDS             2095..>2799
                     /locus_tag="P5558_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HRY27372.1"
                     /translation="MSEAATIETAPAAGIGTFEKWLSLWVAGCIATGLLLGNLLPGVF
                     GFLASLELASVNLVVAVLIWAMVYPMMIGIDFASLRHVGDKPKGLVITLVVNWLIKPF
                     TMAFLGVLFFRHVFASLIPPADAEGYIAGLILLGAAPCTAMVFVWSQLTRGDPAYTLV
                     QVSVNDVVMVFAFAPIVAVLLGVTDIVVPWETLLLSVVLYIVLPLAAGVLTRRRVSAK
                     AGGGRRGEAAVQGFIAR"
BASE COUNT          396 a          877 c         1052 g          474 t
ORIGIN      
        1 agcggcgaga cggggcggcg taaagagtcc tgaccccgtt atacaaacat ttaaaaacaa
       61 acggtggtgc tcgaggaggt gtgcctcctc gaatgctttc tgtcttccct accggtcgtc
      121 gctcgttccg gcagtctggg cttcggccgg gaccgcgttg gcggctgcgg ctccggcggc
      181 ttgggcgtgc tgttctgatg tgccgtcgat gcggacgcgg atttcgcggc gggcgaactg
      241 gattccgtgc tgctcgaacg cgcgctggac tcggtcgtag atttccttcc tgagcacgaa
      301 ctggcggccg gggcgggcca tgaacttgcc gcggatgacg atgccgacgt cgtcgacctc
      361 gtggacgccg tggctctgga agggctgcat gaagtcctgg gcgtagagcg ggtcgccgta
      421 gatgtcattg ccgatggtct tgaagatttt cttcactttc gcgagggccg ggccggtgag
      481 cgccaggagg agggcgatca ggaggaggtg ccgcacggac cggccctcat ttgaccaaaa
      541 gcggcatcat catggtggcg aggctgagga cgaggaagaa gcccgccatg tcggtgacgg
      601 tggtgagcag cggcccggag gcgacggccg ggtcggtgcc gaagcgcttg agcacgaggg
      661 gcacgatgcc gccgatcgat accgcgatca tggtgttgat ggcgagggcc gtgccgatga
      721 ccacgccgag ccagggattg cccttccaga cccagcagac gacgccgatc agaaggccga
      781 ggacgaggcc gttcatgacg ccgaccgaga tctccttgag ccagacacgg gcgatgtcgg
      841 tggggcgggt gaggcccagg gccagctcgc ggagcgagac ggcgacggcc tggttgccgg
      901 agcagccgct catgtcggag accatgggca ggaagacggc gatggcgatg acggcggcca
      961 gcgtctcctc gtaggccgag atgacgctgg cggcgatgat gttgaggacg atgttggcgc
     1021 tgagccaggc gaggcggcgg cgggcgcgga cgaaggtcgg catggagcgc agctcgtcgc
     1081 cgatgacgcc ctgcatcttg aggctgtcgg attcggtgcg ctcctggacg gcttcggcga
     1141 tcgcgatgcg cgagacgacg ccgatcagca cgcccctggc gtcggtgacg gggatgccga
     1201 ggaacgggtg ctcgtcgaag aggttctcga gctcgtcgat ggaggcggtg gggggcaccg
     1261 agatcgcctt ggccatgacg tcgcggagca ccgtgccgcg ccggatggtg agcagggtgc
     1321 ggagcgagac gacgccctgg aggcggccct cggcatcgac gacatagggg tgctggccgc
     1381 gatagcgctc gaaatcctcg tcctcgacgg tgaggccgcg caggacggcg ccgaccgtct
     1441 cgtcgacgcg aaaggcgaag gtctcggcca ccatcaggcc gccggcggtg tcgctgtcgt
     1501 actggatgag gcggcggacg tcggccgcgt ccgccgggtc catccgggcg aggatggcct
     1561 cggcgtccgc ggtgtcgagc tcgtcgatca ggtcgacctg atcgtcgctg tcgagctcct
     1621 cgacgatgct ggcggcgacc ccggcgtcga gctcctcgac gatctcggcg gcgagctcgg
     1681 cgggcgcctc ctcgacgatc cgggcggccg cctcggcgct gaccaggccg agcatccggt
     1741 tgcgatcctc gaccgacagc tcgagcgtga ggcgcagcgc ctcgctcggc ggctgctcgg
     1801 cgagcagggc ctcgagggcg ggcccgtcgt cgcgctccag cgcctcgtcg agcatgtcct
     1861 cgtcggaacg cggcggcgct tcggtctcgg tcatcggccc ctcctgcggc acggcgtggc
     1921 tggcgcgcag tgtagagaaa tcccggcctt tggcgaccgg cgggcggggc gcgggcgacg
     1981 ggcttgatcg ccgccggcgg ctcgactatc cgtcgccccg cggcagcacc gccttggggc
     2041 gtgcccgata tggccggggc agccgcggcg atcgcgcggc gtgaaggcgg cagcatgagc
     2101 gaggcggcaa ccatcgagac ggccccggcg gccgggatcg ggacgttcga gaagtggctc
     2161 tcgctctggg tggcgggctg catcgccacg ggattgctgc tgggcaacct cctgcccggc
     2221 gtcttcggct tcctcgcctc gctcgagctg gcgtcggtca acctcgtcgt cgccgtgctg
     2281 atctgggcca tggtctatcc catgatgatc ggcatcgact tcgccagctt gcggcatgtc
     2341 ggcgacaagc cgaaggggct ggtgatcacg ctggtcgtca actggctgat caagcccttc
     2401 accatggcgt tcctcggcgt gctcttcttt cgccacgtct tcgccagcct cattccgccg
     2461 gccgatgccg agggctacat cgccgggctg atcctgctcg gtgccgcacc ctgcaccgcc
     2521 atggtcttcg tctggtcgca gctcacccgg ggcgaccccg cctacacgct ggtccaggtc
     2581 tcggtgaacg acgtcgtcat ggtcttcgcc ttcgcgccga tcgtggcggt gctgctgggc
     2641 gtcaccgaca tcgtggtgcc ctgggagacg ctcttgctct ctgtcgtgct ctatatcgtg
     2701 ctgccgctcg cggcgggcgt gctgacgcga aggcgggtgt cggcaaaggc ggggggcggg
     2761 cgacggggcg aggcggcggt gcagggcttc atcgcccgg
//
LOCUS       DAOTFI010000394         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_42658_length_2798_cov_5.992709, whole genome shotgun sequence.
ACCESSION   DAOTFI010000394 DAOTFI010000000
VERSION     DAOTFI010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42658_length_2798_cov_5.992709"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /gene="iolD"
                     /locus_tag="P5558_23615"
     CDS             complement(<1..1744)
                     /gene="iolD"
                     /locus_tag="P5558_23615"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HRY27373.1"
                     /translation="MRTVRLTMAQALVRWLVAQRTVVDGHEVPLFPGVFAIFGHGNVT
                     CLSEALEAVQDDLPTWRGQNEQSMALAAIGYNKARKRRQIMIATSSIGPGATNMVTAA
                     AVAHTNRLPLLLLSGDYYVSRAPDPVLQQVEHFGDPTISVNDAFKPVVRYWDRITRPE
                     QIIQSLPQALAMMLDPADCGPAFIGLCQDVQAEAFDYPERFFETRVHRIERRRADTVQ
                     LKMAVQLLKSAKKPLIIAGGGVRWSDAEAVLAAFAEKHGIPVGETLNGRTVLLHDHPM
                     NVGPIGIMGSSSANELAAEADVVLAVGTRLQDFTTGSWTLFAPDTHFIGLNAARFDAV
                     KHQSLPVVGDAKVGLEELGAALGSWAAPTAWREHGAALYAKWNAYVEHRSGPTNAALP
                     TYAHVVGAVSRLAGERDLALTAAGGMPGELSMCWRAKGIGTFDCEFGYSCMGYELAGG
                     WGAAMADPHREVFVMVGDGSYLMMNSDIYSSVLTGHKLIVVVCDNGGFLVIDRLQRAK
                     GVPSFNNSLDTCRTADGAFRVDFAKHAEAMGAIGIRVQSIGDLEEAVRTAKAADRTTV
                     IHIDVHPTDWTGNDN"
     gene            1911..>2798
                     /gene="iolG"
                     /locus_tag="P5558_23620"
     CDS             1911..>2798
                     /gene="iolG"
                     /locus_tag="P5558_23620"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HRY27374.1"
                     /translation="MLRFALFGAGRIGRMHADNLVRHPKAELAYVYDVVGEAAKGCAA
                     RHGAMVASSIEQALGDSAVDAVLIASSTDTHIDLIQASVEAGKAVLCEKPIDLDIARV
                     DACWQRIRGRDPKVMIGFNRRFDPSFKAVHDAIRAGEIGDLRQVVITSRDPEVPPVAY
                     MQVAGGLLRDMTIHDFDLARYLLGEEPLKVSCFAQALIAQEVKELNDHDTAMIIMETA
                     SGRQCCINNYRRAVYGYDQRIEAIGETGMLKAENRRPNTLERWDEKATGQKAPILHFF
                     IERYQEAYNAEIQAFVDAVE"
BASE COUNT          463 a          998 c          926 g          411 t
ORIGIN      
        1 aattgtcgtt gccggtccag tcggtcgggt gcacgtcgat gtggatcacc gtggtgcggt
       61 cggcggcctt ggcggtgcgc accgcctcct cgagatcgcc gatgctctgc acgcggatgc
      121 cgatggcccc catcgcctcg gcatgcttgg cgaaatcgac gcggaaggcg ccgtcggccg
      181 tgcggcaggt gtcgagcgag ttgttgaagg aggggacacc cttcgcccgc tgcagccggt
      241 cgatgacgag gaacccgcca ttgtcgcaga ccacgacgat cagcttgtga cccgtgagca
      301 ccgacgaata gatgtcggag ttcatcatca ggtagctgcc gtcgccgacc atgacgaaga
      361 cctcgcggtg cgggtcggcc atggccgccc cccagccgcc ggccagctcg tagcccatgc
      421 aggaatagcc gaactcgcag tcgaacgtgc cgatgccctt ggcccgccag cacatcgaaa
      481 gctcgcccgg catgccgccc gcggcggtca gcgccaggtc gcgctcgccg gcgagccggc
      541 tcaccgcacc cacaacgtgc gcgtaggtcg gcagggccgc gttggtcggc cccgagcgat
      601 gctcgacata ggcgttccac ttggcataga gcgccgcccc gtgctcgcgc caggcggtgg
      661 gcgccgccca ggaacccaag gccgcgccca gctcctcgag ccccaccttc gcgtcgccga
      721 ccacgggcag gctctggtgc ttgaccgcgt cgaagcgcgc cgcgttcagg ccgatgaaat
      781 gcgtgtccgg ggcgaagagc gtccaggagc cggtggtgaa gtcctgcagc cgcgtgccga
      841 cggccagcac cacgtcggcc tcggcggcga gctcgttggc ggaggacgag cccatgatgc
      901 cgatcggccc gacattcatg ggatggtcgt ggagcagcac ggtccgcccg ttcagcgtct
      961 cgccgaccgg gatgccgtgc ttctcggcga aggcggcgag cactgcctcg gcgtcgctcc
     1021 agcgcacccc gccgccggcg atgatgagcg gcttcttcgc cgacttgagg agctggaccg
     1081 ccatcttgag ctggaccgtg tcggcccgcc gccgctcgat gcgatgcacc cgcgtctcga
     1141 agaagcgctc cggatagtcg aatgcctcgg cctgcacgtc ctggcagagg ccgatgaagg
     1201 cgggcccgca atccgcgggg tccagcatca tggcgagcgc ctggggcagg gactggatga
     1261 tctgctccgg ccgggtgatc cggtcccagt agcgcaccac cggcttgaag gcgtcgttca
     1321 ccgagatcgt cgggtcgccg aaatgctcga cctgctgcag caccgggtcg ggggcgcggc
     1381 tgacatagta gtcgcccgag agcagcagga gcggcaggcg gttggtgtgg gcgacggcgg
     1441 ccgcggtcac catgttggtc gcacccggcc cgatcgagct cgtcgcgatc atgatctgcc
     1501 tgcgcttcct cgccttgttg tagccgatcg cggccaaggc catggactgc tcgttctgcc
     1561 cgcgccaggt cggcaggtcg tcctgcacgg cctccagcgc ctcggagagg caggtcacgt
     1621 tgccgtggcc gaagatggcg aagacgcccg ggaagagcgg cacctcgtgg ccgtccacca
     1681 ccgtccgctg ggccaccagc cacctcacca gcgcctgggc catggtcagc cgaaccgtcc
     1741 gcatagcacc cctccctcag aattgccgag ccgcccgggc ggcgctcaac ctctagcagg
     1801 ccgcgccggc gccgtgaagc cggggaatga aacgcacgtt ccatttgcgt cgacaagcgg
     1861 aacaaactat tcaggcggcg caggcccgga ccccagggag gaacatcgcc atgctgcgct
     1921 tcgcgctctt cggcgccggc cggatcggcc ggatgcacgc cgacaatctc gtccgccacc
     1981 cgaaggccga gctcgcctat gtctacgacg tggtgggcga ggcggccaag ggctgcgcgg
     2041 ccaggcacgg ggccatggtc gcctccagca tcgagcaggc gctcggcgac agcgcggtcg
     2101 atgccgtgct catcgcatcc tcgaccgaca cccacatcga cctgatccag gcctcggtcg
     2161 aggccggcaa ggccgtcctc tgcgagaagc ccatcgacct cgacatcgcc cgcgtcgatg
     2221 cctgctggca gcgcatcagg ggtcgcgacc ccaaggtcat gatcggcttc aaccgccgct
     2281 tcgacccctc cttcaaggcc gtccacgacg ccatccgggc cggcgagatc ggcgacctcc
     2341 gccaggtcgt catcaccagc cgcgaccccg aggtgccgcc cgtcgcctac atgcaggtgg
     2401 ccggcggcct gctccgcgac atgaccatcc acgacttcga cctcgcccgc tacctgctcg
     2461 gcgaggagcc gttgaaggtc tcgtgcttcg cccaggcgct gatcgcccag gaggtgaagg
     2521 aactgaacga ccacgacacg gccatgatca tcatggagac cgcgtcgggc cggcagtgct
     2581 gcatcaacaa ctaccgccgc gcggtctacg gctacgacca gcggatcgag gcgatcggcg
     2641 agaccggcat gctcaaggcc gagaaccgcc gccccaacac cctggagcgc tgggacgaaa
     2701 aggccaccgg gcagaaagca cccatcctcc acttcttcat cgagcgctac caggaagcct
     2761 acaacgccga gatccaggcc ttcgtcgacg ccgtcgag
//
LOCUS       DAOTFI010000395         2767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_43269_length_2767_cov_7.092183, whole genome shotgun sequence.
ACCESSION   DAOTFI010000395 DAOTFI010000000
VERSION     DAOTFI010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43269_length_2767_cov_7.092183"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="P5558_23625"
     CDS             complement(<1..312)
                     /locus_tag="P5558_23625"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27375.1"
                     /translation="MATFEVALSGDFRLPDGGAAYPSFDLGPLQANPDVRVRYVDPVD
                     GVMPAAALEDVDALILLVPRFDATSVPKSGRLAVVARFGVGFDSVDVPVCTANDIALV
                     TT"
     gene            complement(317..1153)
                     /locus_tag="P5558_23630"
     CDS             complement(317..1153)
                     /locus_tag="P5558_23630"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY27376.1"
                     /translation="MTGEVAALLDRKGLKLGHYVGEFTTPGIGYIMKEAGADFVFFDM
                     EHSGNSFETVQRCLRYFEAADMPVFTRVPSDRSDHIARALDAGADAIVIPMVGSAKQA
                     RSVLEKMKYTPIGKRGLALALANDRYRMQPVSQALAAANARTRFVALIETAEGIENVD
                     EIAAVDGVDVLWVGHLDLTASMGIPGQFDHPDYKKALAKVLKAGQKHGLGLGRMVGDI
                     PSGVGLAEEGWDLIAYNGDVWLLQNALRQGIDGIRSGYADVAKAAKKGAAKKTKKKAK
                     GG"
     gene            complement(1150..1998)
                     /locus_tag="P5558_23635"
     CDS             complement(1150..1998)
                     /locus_tag="P5558_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRY27377.1"
                     /translation="MTRIALLGAGGKMGVRLATNLLGSPFEVAHVEVSEVGRKRLKDA
                     IGADCVDQAQALGNADVVLMAVPDALIGKIAHTFVDQLQPGCAVIMLDAAAPHAGELP
                     KRDDITYFVTHPCHPSIFRYEPTLEAQKDYFGGVAAGQGIVSALMQGPEEHFALCEEI
                     ARTIYKPVTRSHRVTVEGLALLEPGLSETIGATLVTALREATDAVVARGVPKEAAMDF
                     MLGHLGIEMGILFEDFPGMFSDGAKLAIANARPKLLRDDWLNVLEPAEIEASVKEICN
                     PKGAAS"
     gene            complement(2084..>2767)
                     /locus_tag="P5558_23640"
     CDS             complement(2084..>2767)
                     /locus_tag="P5558_23640"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HRY27378.1"
                     /translation="PHCPFEARARAVMQVIDRHAQATGKRVMFAFNITGELDEMRRRH
                     DLVEALGGTCVMASLNSVGIVGMVELGRHSRLPIHAHRNGWGYLSRHPLLGWSFPAWS
                     KIWRLAGADHMHVYGIRNKFCEPDDSVIASARSVLTPMWDEKPCISMPVFSSGQTAGQ
                     AHDTYAAIGTTDLIFAAGGGLMGHPDGIEAGVASLRQAWDAAAAGIPLATAAAAHKEL
                     RTALEALGR"
BASE COUNT          408 a          913 c          989 g          457 t
ORIGIN      
        1 cgtggtgacg agggcgatgt cgttcgccgt gcagaccggc acgtcgacgc tgtcgaagcc
       61 gacgccgaag cgcgctacca cggcgagccg gccgctcttc ggcacgctcg tcgcatcgaa
      121 gcgcgggacg aggaggatga gcgcgtcgac atcctcgagg gcggcagccg gcatgacgcc
      181 gtcgaccgga tcgacgtagc ggacccgcac gtcgggattg gcctggagcg ggccgagatc
      241 gaacgaggga taggcggcac cgccgtcggg aagccggaaa tccccgctca gggctacttc
      301 gaaggtcgcc acgtcgtcag cctcccttgg ctttcttctt cgtcttcttc gcggcgccct
      361 ttttggcggc tttcgccacg tccgcatagc ccgaacgaat gccgtcgata ccctggcgca
      421 acgcgttctg cagcagccag acatcgccgt tataggcgat caggtcccag ccttcctcgg
      481 ccaggccgac gccggagggg atgtcgccga ccatccggcc gaggccgagg ccgtgcttct
      541 ggcccgcctt caggaccttg gccagggcct tcttgtagtc ggggtgatcg aactggcccg
      601 ggatgcccat gctggcggtg aggtcgagat ggccgaccca gagcacgtcg acgccgtcga
      661 ccgcggcgat ctcgtcgacg ttctcgatac cctcggcggt ctcgatcagg gcaacgaagc
      721 gggtccgggc gttggcggcg gccagcgcct ggctgaccgg ctgcatcctg tagcggtcat
      781 tggccagggc gagcgccaga ccgcgcttgc cgatcggcgt atacttcatc ttctcgagga
      841 cggagcgggc ctgcttggcc gagccgacca tcggaatgac gatcgcatcg gcgccggcgt
      901 cgagggcgcg ggcgatgtgg tcggagcgat cggacgggac ccgggtgaag accggcatgt
      961 cggccgcctc gaaatagcgc aggcagcgct gcaccgtctc gaacgagttg ccgctgtgct
     1021 ccatgtcgaa gaacacgaag tccgcgccgg cctccttcat gatgtagccg atgccgggcg
     1081 tggtgaactc gcccacatag tgcccgagct tcagcccttt gcggtcgagc agggcggcga
     1141 cttcgccggt catgatgcgg ctcccttcgg gttgcagatc tccttgacgc tcgcctcgat
     1201 ctcggcgggc tcgagcacgt tcagccagtc gtccctgagc agctttggcc tggcgttggc
     1261 gatggcgagc ttcgcaccgt cggagaacat gcccgggaag tcctcgaaga ggatgcccat
     1321 ctcgatgccg agatggccga gcatgaagtc catggcggcc tccttgggca cgcccctggc
     1381 gacgaccgcg tcggtggctt cgcgcagcgc cgtgaccagc gtggcgccga tggtctcgga
     1441 gagcccgggc tcgagcaggg cgaggccctc gacggtcacg cggtgcgagc gggtcaccgg
     1501 cttgtagatg gtcctcgcga tctcctcgca gagggcgaaa tgctcctccg gcccctgcat
     1561 cagggcgctg acgatgccct ggccggccgc cacgccgccg aaatagtcct tctgcgcctc
     1621 gagcgtcggc tcgtagcgga agatcgaggg atggcagggg tgggtgacga agtaggtgat
     1681 gtcgtcgcgc ttcggcagct cgccggcatg gggggccgcg gcgtccagca tgatcacggc
     1741 gcagcccggt tggagctggt cgacgaaggt gtgcgcgatc ttgccgatga gcgcatccgg
     1801 caccgccatc agcaccacgt cggcattgcc gagagcctgg gcctggtcga cgcagtcggc
     1861 gccgatggcg tccttcaggc gcttgcggcc gacctcgctg acctccacat gggcgacctc
     1921 gaagggcgag cccaggagat tggtggcgag gcgcacgccc atcttgccgc cggccccgag
     1981 cagcgcgatc ctggtcatct tcctctgtct cccttgtcgt tggccgaccc ttggcgatca
     2041 ggccgaatgg tgtctgcagc tctcgcctgc gcgcgggtcg ggctcatcgg ccgagcgcct
     2101 cgagcgccgt tcgcagctcc ttgtgcgccg ctgccgcggt ggccagcggg atcccggcgg
     2161 cggcggcatc ccaggcctgg cgcaggctgg cgacgccggc ctcgatgccg tccgggtggc
     2221 ccatgagacc accaccggca gcaaagatca ggtcggtcgt gccgatggcg gcgtaggtgt
     2281 cgtgcgcctg gccggcggtc tggcccgagc tgaagaccgg catcgagatg cagggcttct
     2341 cgtcccacat cggtgtcagc acgctccggg cggacgcgat gacgctgtcg tccggctcgc
     2401 agaacttgtt gcggatgccg tagacatgca tgtggtcggc gccggccagc cgccagatct
     2461 tcgaccaggc gggaaaggac cagccgagca gcgggtggcg cgagaggtag ccccagccgt
     2521 tgcggtgggc gtggatcggc aggcggctgt ggcggccgag ctcgaccatg ccgacgatgc
     2581 cgaccgagtt caggctcgcc atgacgcagg tgccgccgag ggcctcgacg agatcgtggc
     2641 ggcggcgcat ctcgtcgagc tcgccggtga tgttgaaggc gaacatcacc cgcttgccgg
     2701 tcgcctgggc atggcggtcg atgacctgca tcaccgcccg ggcgcgggcc tcgaacgggc
     2761 agtgcgg
//
LOCUS       DAOTFI010000396         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_44331_length_2717_cov_4.871525, whole genome shotgun sequence.
ACCESSION   DAOTFI010000396 DAOTFI010000000
VERSION     DAOTFI010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44331_length_2717_cov_4.871525"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="P5558_23645"
     CDS             <1..675
                     /locus_tag="P5558_23645"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY27379.1"
                     /translation="VAGVSAAEPATSLMLARAAAGLGADMVLWQPPGGLAGPALQDSL
                     AGLGEVAPVMLQDLDWHGGGLALETITALAEAVPGFQALKIETAPAGPKYTAVRHALG
                     DRLHLSGGWAAMQMLDGLARGLDAFIPSGVLTAYCRIWRLWAAGERAEAQALFERCLP
                     ILAFSNQDIGVSIAFWKRVRLAAGIFATDRCRPPVQPLDAVQAAIADQLVQRIPALDA
                     AGDGGD"
     gene            complement(683..853)
                     /locus_tag="P5558_23650"
     CDS             complement(683..853)
                     /locus_tag="P5558_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27380.1"
                     /translation="MTDRLMALAAFFTLFGFLGILIWNVPHWDLVLVVLITLLLVAWD
                     FIATLGKQQNGG"
     gene            complement(850..2193)
                     /locus_tag="P5558_23655"
     CDS             complement(850..2193)
                     /locus_tag="P5558_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY27381.1"
                     /translation="MLDLGAIELRDRLARGEFKAVDLAQAALNRIAVLEPELRAWAWL
                     DGEHVLAQARALDAHRATGRPIGPLHGLPVGVKDIIDTARIPTENGCPVDRGRVPVLD
                     AFVVARLKAAGAVLMGKTVSTELAYLHPGPTRNPTNPAHTPGGSSSGSAAAVAAGMVP
                     LAIGTQTGGSVIRPAAYCGITGFKPSFGAIPRTGILSQSPSLDTVGVFARSVEDAALL
                     AELLFGHDPADSATLPAPPPRLLSTALASPPVTPMLAFVRPPGWADADPQTHAAMREL
                     TTALGEQCFEATLPSVFDEAPLIRERINFAEMAKCCFAYERRGRDVLGEETLAAIDAG
                     KAVLARDYLAALDWPHILNAALGPVFDRCDAILTPATPGPAPSGLGSTGSSIFNGIWT
                     LCGTPAITLPLLEAENGLPMGAQLVGRRGDDARLLRTARWLATQALGAAYEGVSR"
     gene            complement(2228..>2717)
                     /locus_tag="P5558_23660"
     CDS             complement(2228..>2717)
                     /locus_tag="P5558_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY27382.1"
                     /translation="VYLTVRTTAMVCWLFVGSYTFSSVFSYLGGEQLISTFVQGLDLT
                     PLQFLLLAQLIIFLLGWPLEWSEIIIIFVPIFLPLLPHFDIDPLFFGILVALNLQTSF
                     LTPPMAMSAYYLKGIAPPHVRLTQIFAGVMPFLFLVFVSMALVYVFPKLVFHLPEVFY
                     GR"
BASE COUNT          454 a          923 c          984 g          356 t
ORIGIN      
        1 gtggccgggg tcagcgccgc cgagccggcg acgagcctca tgctcgcccg ggcggcggcg
       61 gggctcggcg ccgacatggt gctctggcag ccgccgggcg ggctcgccgg gccggccctg
      121 caggacagcc tcgccggcct cggcgaggtg gcgccggtga tgctgcagga cctcgactgg
      181 cacggcggcg ggctcgcgct cgagacgatc accgccctcg ccgaggcggt gcccggcttt
      241 caggcgctca agatcgagac ggcaccggct ggccccaaat acacggcggt gcgccatgcc
      301 ctgggcgatc ggctgcacct ttccgggggc tgggccgcca tgcagatgct cgacgggctg
      361 gcgcggggcc tcgacgcctt catcccctcg ggcgtgctga ccgcctactg ccggatctgg
      421 cgcctctggg ccgcaggcga gcgggccgag gcgcaggccc tcttcgagcg ctgcctgccg
      481 atcctcgcct tctccaacca ggacatcggc gtcagcatcg ccttctggaa gcgcgtgcgg
      541 ctggccgccg ggatcttcgc gaccgaccgc tgccggccgc cggtccagcc gctggatgct
      601 gtgcaggcgg ccattgccga ccagctcgtc cagcgcatcc cggccctcga cgcggcaggg
      661 gacggcgggg actgagccgc cttcagccgc cgttctgctg cttgcccagc gtggcgatga
      721 agtcccaggc caccagcagc agggtgatca gcacgacgag cacgagatcc caatgcggca
      781 cattccagat caggatgccg agaaagccga acaaggtgaa aaaggcggca agcgccatga
      841 ggcgatccgt catcggctga ctccttcata agcggcgccc agcgcctgcg tcgccagcca
      901 tcgcgcggtg cgcaggagcc gggcatcgtc gccgcgccga ccgaccagct gcgcgcccat
      961 cggcaacccg ttctcggcct cgagcagcgg cagcgtgatc gccggcgtgc cgcaaagggt
     1021 ccagatgccg ttgaagatgg agctgccggt gctccccagc cccgagggcg ccgggccggg
     1081 tgtcgccggc gtcaggatgg catcgcagcg atcgaagacc ggcccgagcg ccgcgttgag
     1141 gatgtgcggc caatcgaggg cggcgaggta gtcgcgcgcc agcaccgcct tgcccgcgtc
     1201 gatcgccgcc agcgtctcct cgccgagcac gtcacggccc cgccgctcgt aagcaaagca
     1261 gcatttcgcc atctcggcga agttgatccg ctcgcggatc aggggtgcct cgtcgaagac
     1321 ggagggcagg gtggcctcga agcactgctc gccgagggcc gtggtcagct cgcgcatcgc
     1381 cgcatgggtt tgcggatcgg cgtccgccca cccggggggg cggacgaagg ccagcatcgg
     1441 cgtcaccggt ggcgatgcca gcgctgtcga cagcagcctc ggcggcgggg cagggagcgt
     1501 ggcgctatcg gctggatcgt ggccgaacag cagctcggcg aggagggccg cgtcctcgac
     1561 cgagcgggcg aagacgccga ccgtatccag gctcggcgac tggctgagga ttccggtccg
     1621 cgggatggcg ccgaagctgg gcttgaagcc ggtgatcccg caataggcgg cgggcctgat
     1681 caccgagccg cccgtctggg tcccgatcgc cagcggcacc atgccggccg ccaccgccgc
     1741 ggccgagccc gaggacgagc cgcccggcgt gtgggccggg ttcgtcggat tgcgcgtcgg
     1801 gccgggatgc agataggcga gctcggtgga caccgtcttg cccatcagca cggccccggc
     1861 cgccttgagg cgtgccacca cgaaagcgtc gagcacgggc acccggcccc ggtcgacggg
     1921 acagccattc tcggtcggga tccgcgccgt gtcgatgatg tccttgaccc cgaccggcag
     1981 gccatgcagc ggcccgatcg gccggccggt ggcacgatgg gcatcgagcg cccgcgcctg
     2041 cgccagaacg tgctcgccgt cgagccaggc ccaggcccga agctcgggct cgagcacggc
     2101 gatccggttc agcgccgcct gtgccaggtc gacggccttg aactcgccgc gcgccaggcg
     2161 gtcgcgcagc tcgatggcac cgaggtcgag catcgcgtca tcgacgccac ggctcctggc
     2221 ggtcccccta gcgaccatag aacacctcgg gaagatggaa gaccagcttc gggaagacat
     2281 agacaagggc catgctgacg aagaccaaga acaggaacgg catcacgccc gcgaagatct
     2341 gggtgagccg cacatgcggc ggcgctatgc ccttgaggta gtaggccgac atcgccatgg
     2401 gtggcgtcag gaagctggtc tggagattga gcgccacgag gatgccgaag aagagcgggt
     2461 cgatgtcgaa atgcgggagc agcggcagaa agatcggcac gaagatgatg atgatctcgg
     2521 accactccag cggccagccg agcaggaaga tgatgagctg cgccaggagc aggaactgca
     2581 gcggtgtcag atcgagcccc tggacgaagg tcgagatgag ctgctcgccg cccagatagg
     2641 agaagaccga ggagaacgta taggagccga caaacagcca gcagaccatc gccgtggtgc
     2701 gcacggtgag atagacg
//
LOCUS       DAOTFI010000397         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_44531_length_2708_cov_7.309084, whole genome shotgun sequence.
ACCESSION   DAOTFI010000397 DAOTFI010000000
VERSION     DAOTFI010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44531_length_2708_cov_7.309084"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..76)
                     /locus_tag="P5558_23665"
     tRNA            complement(2..76)
                     /locus_tag="P5558_23665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Glu,seq:ttc)
     gene            208..726
                     /locus_tag="P5558_23670"
     CDS             208..726
                     /locus_tag="P5558_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27383.1"
                     /translation="MASRAGGGGEAIASYFEVHVLRDERWVIDCTSPSAEDALANAQD
                     LARRAEVLGVKVIHERYNPQTDQSAARVVYSAIKPQRPKGRRLPPIAAPAARPTTTPV
                     HHPDDNALSADPASENPQSQMPPSIPATPGRPVVYREPPRETGSWQLFAWASLALAVT
                     ASLLFVLLLLAG"
     gene            complement(790..2610)
                     /gene="recJ"
                     /locus_tag="P5558_23675"
     CDS             complement(790..2610)
                     /gene="recJ"
                     /locus_tag="P5558_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRY27384.1"
                     /translation="MGVARRHVDPEVGGELLVERSVTGKSWRLAAERPDAARSISQQF
                     GLPEVVGRVLAARGMAVATVPGFLEPRLRDWLPDPSHLLDLDRAVERLAAAAAAGERV
                     GIIGDYDVDGATSTALVRRYLEAVGCPVEVRIPDRLTEGYGPNDAAFATLADAGCRLV
                     LCLDNGTTAHAPLAAAAARGLEVIVVDHHTAEAELPAALAVINPNRKDQASALGDLAA
                     VGVAFVLMVALNRALRSAGHFASRVEPDLMAALDLVALGTVCDVVPLTGLNRAFVVQG
                     IKIARQGRNPGLAAMALVAGIETIDDARKFGFILGPRLNAGGRMGESQLGAALLVTED
                     PAIAAELAGRLDLLNTTRQALERETLEAALRAVEPQLRDDQPLLVAAGEGWHAGVIGI
                     VAARLVERFARPALAVALEGGVGKGSGRSIAGFDLGAAVIAARAAGLIEAGGGHPMAA
                     GLTVDVAQLPALQAFLCRRLAATMALSGPAESRTIEPGRAELRVDGALSLAAVNTHLA
                     RKLLQLAPFGAGHDEPRFVLDATRVMQARPVGRGHVSCLLAGPAGPAVRGIAFRSADT
                     GLDRALLGGGALRVVGRIRLDSYQGRERAGLEIEDAAPLG"
BASE COUNT          412 a          974 c          982 g          340 t
ORIGIN      
        1 atggcatccc caacgggaat cgaacccgtg tcttcacctt gaaagggtga tgtcctaacc
       61 gctagacgat ggggacccga gcggtactta acgactggcg atgcgcgaat caacagcggc
      121 gccccggttt ggtcaccgga tcttaaccac tcccgccgca tgatcgatcg aggtggtcga
      181 ggagcgggga tggtcgaggc tcttgcgatg gcaagccgcg ccggtggcgg cggcgaggcg
      241 attgccagct atttcgaggt gcatgtcctg cgcgacgagc gctgggtcat cgactgcacc
      301 tcgccgagcg cggaggacgc gctcgccaat gcccaggatc tcgcccgccg ggcggaggtg
      361 ctgggtgtga aggtcattca cgagcgctac aacccgcaga ccgaccagag cgccgcccgg
      421 gtcgtctact cggccatcaa gccgcagcgc ccgaagggcc gacggctgcc gccgatcgca
      481 gcaccggcgg cacgaccgac cacgacgccc gtccaccacc ctgacgacaa cgcgctgtcc
      541 gccgacccgg cgtccgagaa tccccagtca cagatgccgc cttcgatccc ggcaaccccg
      601 ggccgcccgg tcgtctaccg ggagcctccg cgggaaaccg gatcctggca actgttcgcc
      661 tgggcgagcc ttgccctcgc ggtgacggca tcgctgctct tcgtgctgct gttgctcgcc
      721 ggctgacccc ggacaaccgg tgcggcgctc gaggagagag cccggagcgc catgacgcga
      781 atcgttccgt caaccgagtg gtgcggcgtc ctcgatctcc agcccggccc gctcgcggcc
      841 ctgatagctg tcgaggcgga tgcggccgac gacccgcaag gcaccgccac cgagcagggc
      901 gcggtcgagg ccggtatcgg ccgagcgaaa ggcgatgccc cgcacggccg ggccggccgg
      961 gccggcgagg aggcaggaga cgtggccgcg gccgaccggc cgggcctgca tgacccgggt
     1021 tgcgtcgagc acgaagcgcg gctcgtcgtg gccggcgccg aaaggtgcca gttgcagcag
     1081 cttgcgcgcg aggtgggtgt tgaccgcggc gagcgacagg gcgccatcga cgcgcagctc
     1141 cgcccggccg ggctcgatcg ttcgggattc ggccgggccc gacaaggcca tggtcgccgc
     1201 gagacggcgg cagagaaagg cctgcagcgc cggcagttgg gccacatcaa cggtgagccc
     1261 ggccgccatc ggatgcccgc cgccggcctc gatcaggccg gcggcgcggg ccgcgatgac
     1321 cgcggcaccg aggtcgaagc ccgcaatcga cctgcccgag cccttgccga cgccgccctc
     1381 gagagccacc gcgagggcgg gccgggcgaa acgctcgacc agccgggcgg cgacgatgcc
     1441 gatcacgccc gcgtgccagc cctcgccggc cgcgaccagc agcggctggt cgtcccggag
     1501 ctgtggctcg accgcccgga gcgccgcctc cagcgtctcg cgctcgagcg cctggcgcgt
     1561 cgtgttcagc aggtcgagcc ggccggcgag ctcggcggcg atggccggat cctcggtcac
     1621 cagcaaggct gcgccgagct ggctctcgcc catacgcccg ccggcattga gccgcggccc
     1681 gaggatgaag ccgaacttgc gggcatcgtc gatggtctcg atgccagcga ccagggccat
     1741 cgccgcgagc cccggatttc gcccctggcg ggcgatcttg atgccctgca ccacgaaggc
     1801 ccgattgagg ccggtgagcg gcaccacgtc gcagaccgtg ccgagggcca cgagatcgag
     1861 cgctgccatc agatccggct cgacgcggga ggcgaaatgg ccggcgctgc gcagcgcccg
     1921 gttgagcgcc accatcagga cgaaggcgac cccgaccgcg gcgagatcgc cgagagcgct
     1981 cgcctgatcc ttgcggttcg ggttgatcac ggcgagggcg gccggcagct cggcttcggc
     2041 ggtatggtga tcgacgacga tgacctcgag ccctcgcgcc gcggcggcgg ccaacggggc
     2101 atgcgcggtc gtgccgttgt cgagacagag gaccagccgg cagccggcgt cggcgagcgt
     2161 cgcgaaagcc gcgtcattgg ggccgtagcc ctcggtcagc cgatcgggga tccgcacctc
     2221 gacggggcag ccgacggctt cgaggtagcg gcggacgagc gctgtcgagg tggcgccgtc
     2281 gacgtcgtag tcgccgatga tgcccacccg ctcgcccgcg gccgcggcgg cggcgaggcg
     2341 ctccacggca cgatccagat cgagcagatg cgaagggtcg gggagccagt cgcgcagccg
     2401 cggctcgagg aatcccggca ccgtggcaac cgccatgccg cgggccgcga gcacccggcc
     2461 gaccacctcc ggcaggccga attgctggct gatcgagcgc gccgcgtcgg gccgctcggc
     2521 ggccaggcgc cagctcttgc cggtgaccga ccgctcgacg aggagctcgc cccccacctc
     2581 cgggtcgacg tgccggcgcg cgacccccac gctcaaggcg gggccgtcct caacggccga
     2641 aatgcgggac gatggcgctc ttgcgcggca ggttgtgctc ggcctcgatc agccggacgg
     2701 tgccgctc
//
LOCUS       DAOTFI010000398         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_46528_length_2622_cov_5.219322, whole genome shotgun sequence.
ACCESSION   DAOTFI010000398 DAOTFI010000000
VERSION     DAOTFI010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46528_length_2622_cov_5.219322"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1274
                     /locus_tag="P5558_23680"
     CDS             99..1274
                     /locus_tag="P5558_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRY27385.1"
                     /translation="MTRTAPPLAPVRIRRIVAAPLLGESPKGGWSNEIRAEDSVHTLI
                     AVKTDAGITGHGSVFTDGRLVQAALGVLEPLFLGENALEPERVSEKLHQNTFWMGRGG
                     TLTHAISGIDIALWDILGQATGLSIGRLAGGRHQERVLPYCSLLMDEPEVMADEIAKY
                     REEGFRAFKIGWGPFGRRDDAKLDAAIVAAAKSQLPAGGKLFVDAGASDAWWPNGLKW
                     ALRTADMLADHEVDWFEEALVPDALEDFVTLRQASRLPITGGEVLTRRQAFIPFITRG
                     AFDIVQPDVTKVGGLSEQRRIAWLARDFGVKYVGHGWNTALGLAADLQLATALPNVDL
                     VEYIGGSAYVDRIIETPFGLDADGMLAIPDEPGIGVTLDPEKVARYTPDAAAFFATD"
     gene            1283..>2622
                     /locus_tag="P5558_23685"
     CDS             1283..>2622
                     /locus_tag="P5558_23685"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY27386.1"
                     /translation="MAPARTIPTERRHQHWNLSKAQAVSTGGIVTSQSAIAAAAGARA
                     LAAGGNAVDAAIVTAMALHVTEPWSSGLGGGGFMLLDLPGTGAQAINFSMRAAGDVDP
                     SRYPTTGTTRHGPFQWPLVVEERNTVGYDSILIPGAVDGFALALEKFGKLSWGDALEH
                     VIPLAEEGLPVGWYTSLAIAVEAHQIARFPHTRDVYLRGGCFVPVSESDGEKVHIEDP
                     LMPDFLRRLQRNGARDFYAGEIGAAIVADMQAGGRNWTLADLDSYRARLAAPEASPYR
                     GFELMGVPGLNGAPTVMRFFRQMEGRHKPKGSAPGPEDYATFAALMRELWEERYQSMG
                     DDQAQDKCTTHVSVVDKAGGMASLTNTMGSRFGSKVSLPSTGLLMNNGMFWFDPEPGR
                     TNSIGPGKWPLTNACPMLARRDGRPVLALGAAGGRRVPPTVLQLLSFCIDYGMDL"
BASE COUNT          394 a          928 c          892 g          408 t
ORIGIN      
        1 tggtgctccc tcggtcgcgc ttgaagtgta tgattgtata cgatatcagg cgagtcaacg
       61 tccccgcact gcctgttcca ccgatcccga aagcgcgcat gacccggacc gctccacctt
      121 tggcccccgt ccgcatccgg cgcatcgtcg cggccccgct gctcggcgag agccccaagg
      181 gcggctggtc gaacgagatc cgggcggagg attcggtcca cacgctgatc gcggtcaaga
      241 ccgatgccgg catcaccggc catggcagcg tcttcaccga cggccggctg gtccaggcgg
      301 cgctcggcgt gctggagccg ctgtttctcg gcgagaatgc gctggagccc gagcgggtct
      361 cggagaagct gcaccagaac acgttctgga tggggcgcgg cgggacgctg acccacgcca
      421 tcagcggcat cgacatcgcc ctttgggaca tcctcgggca ggccacgggc ctctccatcg
      481 gcaggctcgc cggcgggcgg caccaggagc gggtgctgcc ctattgctcg ctgctcatgg
      541 acgagccgga agttatggcg gacgaaatcg cgaaatatcg cgaagagggc tttcgcgcct
      601 tcaagatcgg ctggggccct ttcggcaggc gcgacgatgc gaagctggat gccgccatcg
      661 tcgccgcggc gaagagccag ctcccggccg gcggcaagct cttcgtcgat gccggcgcca
      721 gcgacgcctg gtggccgaac ggcctgaaat gggcgctgcg gaccgccgac atgctggccg
      781 accacgaggt cgactggttc gaggaggcgc tcgtcccgga cgcgctcgag gacttcgtca
      841 cgctcaggca ggcgtcgcgc ctgccgatca ccggcggcga ggtgctgacc cgccgccagg
      901 ccttcatccc cttcatcacg cggggcgcct tcgacatcgt ccagcccgac gtcaccaagg
      961 tcggcggcct ctccgagcag cgccgcatcg cctggctcgc ccgggacttc ggtgtgaagt
     1021 atgtgggcca cggctggaac acggcactcg gcctcgccgc cgacctgcag ctcgccaccg
     1081 ccctgccgaa tgtcgatctg gtcgagtata tcggcgggtc ggcctatgtg gaccggatca
     1141 tcgagacgcc cttcggccta gacgcggacg gcatgctggc gatccccgat gagcccggca
     1201 tcggcgtcac cctcgatccc gagaaggtgg cccgctacac gccggacgcc gccgccttct
     1261 tcgctaccga ttgaggtgtt ccatggcgcc tgcaaggacg atcccgaccg agcggcgcca
     1321 ccagcactgg aatctgagca aggcccaggc ggtctcgacc ggcggcatcg tcacctcgca
     1381 gagtgccatc gccgcggcgg ccggggcgcg ggccctcgcc gccggtggca acgcggtcga
     1441 tgccgccatc gtcaccgcca tggcgctgca cgtcaccgag ccctggagca gcggcctcgg
     1501 tggtggcggc ttcatgctgc tcgacctgcc gggcacgggt gcgcaggcca tcaacttctc
     1561 gatgcgcgcg gcgggcgatg tcgacccctc gcgctatccc accaccggca cgacccgcca
     1621 cgggcctttc cagtggccgc tcgtggtcga ggagcgcaac accgtcggct acgattcgat
     1681 cctgatccct ggggcggtcg acggcttcgc gctggcgctg gagaagttcg gcaagctctc
     1741 ctggggcgat gcgctggagc atgtcatccc gctcgccgag gagggcctgc cggtcggctg
     1801 gtacacgtcg ctcgccatcg ccgtcgaggc gcaccagatc gcccgcttcc cgcatacgcg
     1861 cgacgtctat ctgcggggcg gctgcttcgt gccggtcagc gagagcgacg gcgagaaggt
     1921 gcacatcgag gacccgctga tgccggactt cctgcgccgg ctgcagcgca acggagcccg
     1981 ggatttctac gccggcgaga tcggtgccgc gatcgtcgcc gacatgcagg cgggcgggcg
     2041 gaactggacg ctcgccgacc tcgattccta ccgtgcccga ctcgccgccc ccgaggccag
     2101 cccctatcgc ggcttcgagc tgatgggtgt gcccggcctc aacggtgcgc ccaccgtgat
     2161 gcgcttcttt cgccagatgg aggggcgcca caagcccaaa ggttcggcgc cgggaccgga
     2221 ggactatgcc accttcgcgg cgctgatgcg cgagctctgg gaggagcgct accagagcat
     2281 gggcgacgac caggcccagg acaaatgcac gacccatgtg agcgtcgtgg acaaggccgg
     2341 cggcatggcg tcgctcacca acaccatggg gtcgcgcttc ggctccaagg tttcgctgcc
     2401 ctcgaccggc ctgctcatga acaacggcat gttctggttc gacccggagc cgggccggac
     2461 caactccatc ggccccggca agtggccgct caccaatgcc tgcccgatgc tggcgcggcg
     2521 ggacgggcgg ccggtcctgg cgctcggggc ggcgggcggc aggcgggtgc cgccgaccgt
     2581 gctgcagctc ctctccttct gcatcgacta cggcatggac ct
//
LOCUS       DAOTFI010000399         2561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_47974_length_2561_cov_6.027135, whole genome shotgun sequence.
ACCESSION   DAOTFI010000399 DAOTFI010000000
VERSION     DAOTFI010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47974_length_2561_cov_6.027135"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="P5558_23690"
     CDS             complement(<1..274)
                     /locus_tag="P5558_23690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY27387.1"
                     /translation="MRPFSILIVDDARSERMLLRQAIYDARRNLRLFEAADPRSARDI
                     FRRHPLDLVFLDVELIAASGLDLLRELRLADPALPIVMVTHHSTQDV"
     gene            388..1314
                     /locus_tag="P5558_23695"
     CDS             388..1314
                     /locus_tag="P5558_23695"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRY27388.1"
                     /translation="MSAAFGAIAPVFLLLALGFALGRRQFPAVGFWPAVERLVYWCLF
                     PALLLRTTAGVESGGAVMLGIALAVLGPIGLVMTLVTLGAPLLGLAGRMIPAVAQAAT
                     RQNVYIAFAAAQGLWGEPALAPLAIAVAAYVPGVNVASALLLGLHAATRPRPAVLARA
                     LLLNPLVLASAAGLVLGQTGWHPPFVLDETLRVLAMASLPLALLATGAGLVWRGAGGR
                     ELAWLAAASAFKLAGLPLLTLATALALGLPPLPLAVVVLFASLPLSPTAYVQTRAMGG
                     HAGFMANAVALQTLLAMASLPVVMGLLESWNP"
     gene            1311..2216
                     /locus_tag="P5558_23700"
     CDS             1311..2216
                     /locus_tag="P5558_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY27389.1"
                     /translation="MTRAGILAIGAPALFVFLWSTGFISAKLGLPYIEPMTFLVLRFL
                     ATTIGFLALVLWLGLAWPAARIVLHQCVAGILLHGVYLGGVFASLAAGVEAGASALIV
                     GLQPLLVAAVAAPLLGERVASRQWLGLALGLAGVGLVVFEKLGQGLGTPVGVLLSVLA
                     LLGITTGTLYQKRFAAGVDLRVGNVFQFGAATLVTGFLAFTFEERTVDWQPPLIFALA
                     WSILVLSFGAVTLLYVLIREGAAARVSSLFFLVPPTTALMAWALFDEWLPPLAILGMA
                     VTMAGVALVNRPAARASPHRDPPRR"
     gene            complement(2164..>2561)
                     /locus_tag="P5558_23705"
     CDS             complement(2164..>2561)
                     /locus_tag="P5558_23705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY27390.1"
                     /translation="AQRRCTPNPFSSKFRTSPQAPPLVSLAYASSISLVLSSLPLFWI
                     WQPMAWADWGAFVVIGALGAMCHGLLIHAFESAPASVLAPLGYAEMIMAVAVGYLWFG
                     DFPDAMTWFGIAIIASAGLYVGWRERLAG"
BASE COUNT          308 a          959 c          854 g          440 t
ORIGIN      
        1 cgacgtcctg ggtgctgtga tgggtgacca tgacgatcgg cagcgccggg tcggcgaggc
       61 gcagctcgcg caggagatcg aggccgctgg cggcgatgag ctcgacatcg aggaacacca
      121 gatcgagcgg gtggcggcga aaaatgtccc tggccgagcg cgggtcggcg gcctcgaaca
      181 ggcgaagatt gcgccgcgcg tcgtagatgg cctgacgaag cagcatccgc tcgctgcgcg
      241 catcgtcgac gatgaggatc gagaaaggcc gcatgaagct cccggcgcac tcctgggctg
      301 gttctgcgcc gatcctagcc cccatccttt gccaattcct taacgcggcg ctagccacgt
      361 cgaccgcgca tcaatagatg gtcgcccatg tccgccgcct tcggggcgat cgcacccgtc
      421 ttcctcctcc tggccctggg cttcgccctc gggcggcgcc agtttccggc ggtcggcttc
      481 tggccggcgg tcgagcggct ggtctactgg tgcctcttcc cggcgctgct cctgcgcacc
      541 acggcgggtg tcgagagcgg cggtgccgtg atgctgggga tcgcgctcgc cgtgctaggg
      601 ccgatcggcc tcgtcatgac gctggtgacc ctgggtgccc cgctgctcgg cctcgccggc
      661 cggatgatcc cggcggtggc ccaggccgcc acccgccaga acgtctatat cgccttcgcc
      721 gccgcccagg gcctctgggg cgagccggcc ctggcgcccc tcgccatcgc ggtcgccgcc
      781 tatgtcccgg gggtgaacgt cgccagcgcc ctcctgctcg gcctgcatgc ggcgacgcgg
      841 ccccggccgg cggtgctggc ccgggccctt ctcctgaacc cgctggtgct cgcctcggcg
      901 gccggcctcg tgctcggcca gaccggctgg cacccgcctt tcgtgctcga cgagacgctc
      961 agggtgctcg ccatggcctc gctgccactg gccctgctcg ccaccggcgc cgggctggtc
     1021 tggcggggtg ccggcggccg ggaactcgcc tggctcgccg ccgccagcgc cttcaagctc
     1081 gccggcctgc cgctcctgac cctggcgacc gccctcgccc tcggcctgcc gcctttgccg
     1141 ctcgccgtcg tcgtgctttt cgcctcgctg cccctctcgc ccaccgccta tgtccagacc
     1201 cgggcgatgg gcgggcatgc cggcttcatg gcgaacgccg tggccctgca gacgctgctc
     1261 gccatggcga gcctgcccgt cgtcatgggc ctgctggaga gctggaaccc atgacccgcg
     1321 ccggcatcct cgccatcggt gcgccggcgc tcttcgtctt cctctggagc accgggttca
     1381 tcagcgccaa gctcggcctg ccctatatcg agccgatgac cttcctggtg ctgcggtttc
     1441 tcgccaccac catcggcttt ctcgcactcg tgctctggct cggcctcgcc tggcccgccg
     1501 cccggatcgt cctgcaccag tgcgtcgccg gcatcctcct gcacggtgtc tatctcggcg
     1561 gcgtcttcgc ctcgctcgcc gccggcgtgg aggccggcgc ctcggcgctg atcgtcgggt
     1621 tgcagccact gctggtcgcc gccgtcgccg cacccctgct cggcgagcgc gtggcatcgc
     1681 gacaatggct cggcctcgcc ctcggcctcg ccggggtcgg cctcgtcgtg ttcgagaagc
     1741 tcggccaggg cctgggcacg ccggtcggcg tcctgctctc ggtgctcgcg ctcctcggga
     1801 tcacgaccgg cacgctctac cagaagcgct tcgccgccgg cgtcgatctc cgggtcggca
     1861 acgtcttcca gttcggtgcc gcgacgctgg tcaccggctt cctggcgttc accttcgagg
     1921 agcggaccgt cgactggcag ccgcccttga tcttcgccct cgcctggtcg atcctggtcc
     1981 tctccttcgg tgcggtgacc ctgctctacg tgctgatccg cgaaggggcg gcggcccggg
     2041 tctccagcct cttcttcctc gtgccgccca ccaccgcgct catggcctgg gcgctcttcg
     2101 acgaatggct gccgcccctg gcgatcctcg gcatggcggt caccatggcc ggcgtggccc
     2161 tggtcaaccg gccagccgct cgcgccagcc cacatagaga cccgccgagg cgatgatggc
     2221 gatgccgaac caggtcatcg catccgggaa atcgccgaac cagaggtagc ccacggcgac
     2281 cgccatgatc atctcggcat agcccagggg tgccagcacc gacgccggcg cgctctcgaa
     2341 agcgtggatc aagaggccgt ggcacatcgc cccgagcgcc ccgatgacca cgaaggcgcc
     2401 ccagtcggcc caggccatcg gctgccagat ccagaagagc gggagcgagg agagcacgag
     2461 gctgatggac gaggcgtagg cgaggctgac cagcggcggc gcctgaggtg aagtcctaaa
     2521 tttgctggaa aatgggttgg gggtacatcg tcgctgagcg t
//
LOCUS       DAOTFI010000400         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_49305_length_2510_cov_5.691242, whole genome shotgun sequence.
ACCESSION   DAOTFI010000400 DAOTFI010000000
VERSION     DAOTFI010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49305_length_2510_cov_5.691242"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="P5558_23710"
     CDS             complement(<1..1757)
                     /locus_tag="P5558_23710"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY27391.1"
                     /translation="MRLIGPAVLAFLLALAGSYVRWQDSGVFRELDYATFDWRLRLRP
                     PEAPGDQVAILVIDDATLQRLGHWPPPRAELAATLEALGEAGAAVIALDLLLLEEATP
                     AGDADPGHTLVTALHRSPKPVLAMAFGFAEPADITLADRLALASNAIPIVRTSPDAVD
                     LLGEPAGAYLPFAPLGEIAALGHVNVFVEPDGELRFLAPAIRFDGAWHPSLPVQAAIR
                     LLDLPRDAIVLDVGRSLRLGDVTAPLDRQSRLVLDPYGPPGTFPQHSLIDLLDGRLDP
                     ALFAGRVVLVGATATGVRDRFGTAFHPQVPGVEIFANALDNLLTGRFIDRSTDVRHLD
                     LALIALGTASGLLMLAPLPAPVLILIAGLLVALPFAIGAVALAFWGLWLNVVFATLGV
                     VGTAGTALAWRAFRLYRRSRRSEARSRHLERYVPSLLRDRTAEGAALVERSQLAAILF
                     CDLEGFTRAAETIDPDRLQPLLQSYYALVEKAASATSGVVAGFSGDGAMLIFGLPEPA
                     ADDPARAIQCGKTLLQAIPAWQKEAEAAGLGGLRLHVGINYGRVRIGHVGGGDQVQLT
                     ANGDVVNVASRLQAMTRET"
     gene            complement(1757..2407)
                     /locus_tag="P5558_23715"
     CDS             complement(1757..2407)
                     /locus_tag="P5558_23715"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY27392.1"
                     /translation="MAVPRPPSPPTRRSCLQGGLALAVAALGQGGTAIAAEAGVVAWL
                     RGTATASSGGSTRILHVGAVVQEGDELRTLEHARISLQLAGGATLAIGEASRLTVTRQ
                     TASPTGGGSVVLDLLDGIVRAVLGPARPDIFEVRGRAAVAAARATDFIVESTSTTTAV
                     FTAAGTVAVTAVDAAGEVVLTAGQGVDATLGEPLGPVKEWGAGRIAEFEARTAVGR"
BASE COUNT          352 a          778 c         1075 g          305 t
ORIGIN      
        1 ttggtttcgc gggtcatggc ctggaggcgg gaggcgacgt tgacgacgtc gccattggcg
       61 gtgagctgga cctggtcgcc gccgccgaca tggccgatgc ggacgcggcc gtaattgatg
      121 ccgacatgga ggcgaaggcc gccgaggccg gcggcttcgg cctccttttg ccaggcgggg
      181 atggcctgga ggagggtttt gccgcactgg atggcgcggg cggggtcgtc ggcggcgggc
      241 tcgggcaggc cgaagatcag catggcgccg tcgccgctga agccggcgac cacgccgctc
      301 gtggcgctcg cggccttctc gaccagggcg tagtagctct ggaggagcgg ctgcaggcgg
      361 tcggggtcga tggtctcggc ggctctcgtg aagccttcga ggtcgcagaa gaggatggcg
      421 gcgagctggc tgcgctcgac caaggcggcc ccttcggccg tgcggtcgcg gagcagggac
      481 ggcacgtagc gctcgaggtg gcggcttcgg gcctcgctgc gccggctccg gcggtagagg
      541 cgaaaggcgc gccaggcgag cgccgtgccg gcggtgccga ccacacccag cgtggcgaag
      601 acgacgttga gccagagacc ccagaaggcc agcgccacgg cgccgatggc gaagggcagc
      661 gcgaccagca ggccggcgat gaggatgagc acgggtgcgg gcaggggggc cagcatgagc
      721 aggcccgagg cggtgccgag agcgatcagg gcgaggtcga gatggcgcac gtccgtcgag
      781 cggtcgatga agcggccggt gagcaggttg tcgagggcgt tggcgaagat ctccacgccc
      841 gggacctgcg ggtggaaggc ggtgccgaag cggtcgcgga ccccggtggc ggtggcgccg
      901 acgaggacca cgcggcctgc gaacagggcg ggatcgaggc ggccgtcgag gagatcgatg
      961 agcgaatgct gcgggaacgt gccgggcggg ccgtacgggt cgagcaccag ccgcgactgc
     1021 cggtcgagcg gggcggtgac gtcgccgagg cggaggctgc ggccgacatc gaggacgatc
     1081 gcgtcgcgcg gcaggtccag aaggcggatc gcggcctgca cgggcaagga cgggtgccag
     1141 gcgccgtcga aacggatggc gggcgcgagg aagcgcagct cgccatcggg ctcgacgaag
     1201 acgttgacgt ggccgagggc ggctatctcg ccgagtggtg cgaaggggag atacgcgccg
     1261 gcgggctcgc ccaggagatc gacggcgtcg ggcgaggtgc ggacgatggg aatggcgttg
     1321 ctggccagcg ccagccggtc ggctagggtg atgtcggccg gctcggcgaa gccgaaggcc
     1381 atggcgagga cgggcttcgg gctccggtgc agggcggtga cgagggtgtg gccggggtcg
     1441 gcgtcgccgg ccggggtggc ttcctcgagc agcaggaggt cgagggcgat gacggcggct
     1501 ccggcctcgc cgagcgcttc gagcgtcgct gcgagctcgg ccctgggcgg cggccagtgg
     1561 ccgaggcgct gcagcgtggc gtcgtcgatg acgaggatcg ccacctggtc gccgggcgct
     1621 tctggcgggc gcagcctgag gcgccagtcg aatgtcgcat agtcgagctc gcggaagacg
     1681 ccactgtcct gccagcggac gtaagagccg gccagggcca ggaggaaggc gaggacggcc
     1741 gggccgatca gccgcatcaa cggccgaccg cggtcctggc ctcgaactcg gcgatgcggc
     1801 ccgcacccca ctccttgacg gggccgagcg gctcgccaag ggtcgcgtcg acgccctggc
     1861 cggcggtgag caccacctcg ccggcggcat ccacggcggt gacggcgacg gtgccggcgg
     1921 cggtgaagac ggcggtggtg gtgctcgtgc tttcgacgat gaagtcggtg gcgcgggcgg
     1981 cggcgacggc ggcccggccg cgcacctcga agatgtccgg ccgggcgggg ccgagcacgg
     2041 cgcggacgat gccgtcgagg agatcgagga cgacgctgcc gccgcccgtg ggcgaggccg
     2101 tctggcgggt gacggtgagg cggctcgcct cgccgatggc gagggtggcg ccgccggcga
     2161 gctggaggct gatcctggcg tgctcgaggg tgcgcagctc gtcgccctcc tgcaccacgg
     2221 caccgacatg gaggatgcgg gtgctgccgc cgctgctggc ggtggcggtg ccgcgcagcc
     2281 aggcgacgac gccggcctcg gcggcgatgg cggtgccgcc ttggccgagc gccgccacgg
     2341 ccaaagcgag gcccccttgc aggcaggagc ggcgggtcgg tggcgagggg ggccggggca
     2401 ctgccatcga ccggtcagag gcgctcgagc tgcaggaggt cgcgcgggcc gacccatgcc
     2461 cggtcgagga ggtcggccgt gaccttggca cccgcctcgt cgcccatggc
//
LOCUS       DAOTFI010000401         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_49458_length_2504_cov_6.275214, whole genome shotgun sequence.
ACCESSION   DAOTFI010000401 DAOTFI010000000
VERSION     DAOTFI010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49458_length_2504_cov_6.275214"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1153)
                     /locus_tag="P5558_23720"
     CDS             complement(146..1153)
                     /locus_tag="P5558_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY27393.1"
                     /translation="MPLLEVANLTVEFPTASGPFRAVDNVSLACDRGEILAIVGESGS
                     GKSVAMLAVMGLLPDSAKVTADHLGFDGREILGISARERRRIIGKDIAMIFQEPMSSL
                     NPCFTVGFQIGETLRFHLGLDRRARRERSIELLDQVGIPAPAERLGQFPHQLSGGMNQ
                     RVMIAMALACKPKLLIADEPTTALDVTIQAQILDLLVDLQRQHDMGLILITHDMGVVA
                     ETAERVQVQYAGQKVEEQPVRELFRDPHHPYTAALLAALPERATRHGKLPSIPGVVPG
                     HGDRPTGCLFSPRCRFVTERCRREVVRQGEELGQALCNYPLRQGEPVDHPGLAPVEVL
                     T"
     gene            complement(1157..2023)
                     /locus_tag="P5558_23725"
     CDS             complement(1157..2023)
                     /locus_tag="P5558_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY27394.1"
                     /translation="MRRAMLAEFWFYFCQNRGAVIGLFVFLAIITVAVFAPLLAPHSP
                     ILQNRDALLLPPFWEEGGSLAYPLGTDAVGRDMLSRLIYGARFSLFIGMVVVTLSVTS
                     GVLLGLAAGYFRGWLDVAIVRLMDVILAFPSLLLALVLVAIIGPGLINAMIAIALVYQ
                     PHFVRLTRGSVMAERGREYVTASRVAGAGRLRLMFLTILPNCLAPLIVQATLAFSSAI
                     LDAAALGFLGMGAQPPTPEWGTMLAEAREFILRAWWVVTFPGLAILITVLAINLMGDG
                     LRDALDPKMKRG"
     gene            complement(2107..>2504)
                     /locus_tag="P5558_23730"
     CDS             complement(2107..>2504)
                     /locus_tag="P5558_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY27395.1"
                     /translation="LPTIVLGTIPLAVIARQTRSAMLEVLGEDYVRTARAKGLGPLRV
                     VGLHALRNALIPVVTTIGLQVGVLLAGAILTETIFSWPGIGKWMVDSVFKRDYAVVQG
                     GLLLIAGVIMLVNLLVDLLYGLVNPRIRH"
BASE COUNT          446 a          800 c          913 g          345 t
ORIGIN      
        1 gattcgccga ccacggcgag cgtgcgcccg gcctcgaggc tgaagctgac gccgttgacc
       61 gcatgcagct cacgcgtctc gcgaaaggct ccttgcgaga ccgtgtagcg gcgggtgagg
      121 tcggtggcct cgacgaccgc ggcgctcaag tcagcacctc caccggggcg aggcccggat
      181 ggtcgacggg ctcaccctgg cgcagcgggt agttgcagag cgcctgtccc agctcctcgc
      241 cctggcggac aacctcgcgc cggcagcgct cggtgacgaa gcggcagcgc ggcgagaaga
      301 gacagccggt cggccggtcg ccatggccgg ggacgacgcc cgggatcgag ggcagcttgc
      361 cgtggcgggt ggcgcgctcg ggcagggccg ccagcagtgc ggccgtgtag gggtgatgcg
      421 ggtcgcggaa gagctctctc accggctgct cctcgacctt ctggccggca tactggacct
      481 ggacgcgttc ggcggtctcg gcgaccaccc ccatgtcatg ggtgatcagg atgagcccca
      541 tgtcgtgctg gcgctgcagg tccaccagca ggtcgagaat ctgcgcctgg atggtgacgt
      601 cgagggcggt ggtcggctcg tcggcgatca gcagcttggg cttgcaggcg agcgccatgg
      661 cgatcatcac ccgctggttc atgccgccag agagctggtg cgggaattgg ccgagacgct
      721 cggcgggggc ggggatgccg acctgatcca gcagctcgat gctgcgctcg cgacgggccc
      781 gccggtcgag cccgagatga aagcgcaggg tctcgccgat ctggaagccc accgtgaagc
      841 agggattgag gctcgacatc ggctcctgga agatcatggc gatgtccttg ccgatgatcc
      901 gtcgccgctc gcgggcactg atgcccagga tctcgcggcc gtcgaaaccg agatggtcgg
      961 cggtgacttt ggcgctatcc ggcagcagtc ccatgacggc cagcatggcc accgatttgc
     1021 ccgagccgga ttcgccgacg atggccagga tctcgccccg atcgcaggcg agcgagacat
     1081 tgtcgacggc gcggaagggc ccggaggcgg tcgggaactc gacggtgaga ttggcgacct
     1141 cgagcagcgg catcgctcag ccccgcttca tcttcgggtc gagcgcatcc ctgagcccgt
     1201 cgcccattag gttgatcgcc agcacggtga tcaggatggc gaggcccggg aacgtgacga
     1261 cccaccaggc ccgcaggatg aactcgcgcg cctcggccag catggtgccc cattcgggcg
     1321 tcgggggctg agcacccatg ccgaggaagc ccagtgctgc agcatcgagg atggcgctgg
     1381 agaaggcgag cgtggcctgg acgatcagcg gcgctaggca gttgggcagg atggtcagga
     1441 acatcagccg gagccgcccg gcgcccgcca ccctggaggc cgtgacgtat tcgcggccgc
     1501 gctcggccat caccgagccg cgggtcaggc gcacgaaatg gggctggtag acgagggcga
     1561 tggcgatcat cgcgttgatc aggccggggc cgatgatcgc caccagcacg agcgccagga
     1621 gcagcgacgg gaaggccagg atgacgtcca tgagacgaac gatggcgacg tcgagccagc
     1681 cgcggaaata gccggcggcg agccccagca gcacgcccga ggtcaccgag agagtgacca
     1741 cgaccatgcc gatgaagagc gagaaccggg cgccatagat caggcgggaa agcatgtcgc
     1801 ggccgaccgc gtcggtgccg agcggatagg ccagcgagcc tccctcctcc cagaacggcg
     1861 gcaggagcag ggcgtcgcga ttctgcagga tggggctgtg aggcgccagc agcggggcga
     1921 agacggcgac agtgatgatg gcgagaaaga cgaagagccc gatcacggca ccgcggttct
     1981 ggcagaagta gaaccagaac tcggccagca tcgctcgccg catcgcccat gcgtcgacgg
     2041 gtgccctgaa ctgactgtcg cttgccgccc cctcttgtgc catggcagcc tcctcgctca
     2101 gggccgtcag tggcggatgc gcgggttgac cagcccgtag agcaggtcga ccaggagatt
     2161 gacgagcatg atgacgccgg cgatcagcag gagcccgccc tgcaccacgg catagtcacg
     2221 cttgaagacg gaatcgacca tccacttgcc gatgccgggc caggagaaga tggtttcggt
     2281 caggatggcg ccggcgagca gcacgccgac ctgcaggccg atggtcgtga ccaccggaat
     2341 gagcgcgttc ctgagcgcgt gcagccccac cacgcgcaac gggcccaggc ccttggcacg
     2401 ggcggtgcgc acataatcct cgcccagcac ctcgagcatc gccgagcgcg tctgccgggc
     2461 gatgacggcg agcgggatgg tgccgagcac gatggtcggc agga
//
LOCUS       DAOTFI010000402         2448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_50964_length_2448_cov_7.227330, whole genome shotgun sequence.
ACCESSION   DAOTFI010000402 DAOTFI010000000
VERSION     DAOTFI010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50964_length_2448_cov_7.227330"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="P5558_23735"
     CDS             <1..793
                     /locus_tag="P5558_23735"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY27396.1"
                     /translation="IFTMVEGMGYAFADGSEGRRRFLRAVQQPLYRLAFSLSNATFVL
                     NPDDRDLLRRRRLAPRRHELVLLNGIGIDLAHFAYAPPPVERPSFLLVGRLIKDKGVV
                     AYADAARRVKAEFPKARFALLGSFDSSPGALAPDQVEAWQREGIIDYLGTVHDVRPVI
                     ADADVFVLPSTYREGVPRSTLEAMAMGRPIITTDAPGCRETVRHGENGYLVAKHDVEG
                     LAVAMRRFAAAPELALPMGLASRRLVEARFDDREVNDTILETMRL"
     gene            complement(826..1794)
                     /locus_tag="P5558_23740"
     CDS             complement(826..1794)
                     /locus_tag="P5558_23740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY27397.1"
                     /translation="MLASAGPHDVYHLPGYHRVAEASGWGEAWLHVVDDGRRWIALPL
                     LLQPIEGAPGRCDATSVYGYAGPITSTAPVGQDLVADFHRTLHQGLVERGAVTLFARL
                     HPLLEQAPVLAGMGELARLGDTVSIDLRLPEEAQLATYRSNHRRDIAKLERKGYRTMV
                     NERPGDLGPFVELYYENMRRVQAQSRYFFDAAHFERLAETLGPALTLALCDLDGEIVG
                     AGLFMRHGPIVQYHLAAVSDRHAGAAPAKQLIDASRRHFRAAGADFLHLGGGRGAIED
                     SLFHFKAGFSPRRHAFRVWRWIIDAPAYEALTAGRPATRFFPAYRG"
     gene            1908..>2448
                     /locus_tag="P5558_23745"
     CDS             1908..>2448
                     /locus_tag="P5558_23745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRY27398.1"
                     /translation="MEAINPPRTLYVRYGKRLLDLAIAVPLALLLSPLMLVVAIVVRW
                     RLGAPVLFRQDRIGKDDRHFRLAKFRTMTDRRDASGALLPDGDRLTPLGLFLRKTSLD
                     ELPQLWNVLRGDMSLIGPRPLLVEYLPRYNARQRCRHKVMPGITGLAQVAGRNLLSWE
                     ARFEHDVTYVREIGPALDCR"
BASE COUNT          371 a          871 c          831 g          375 t
ORIGIN      
        1 gatcttcacc atggtcgagg gcatgggcta cgccttcgcc gacggcagcg agggccgccg
       61 acgctttctc cgcgccgtcc agcagccgct ctatcggctc gcattctcgc tctccaatgc
      121 gaccttcgtc ctcaatccgg acgatcgcga tctcctgcgc cggcggcggc tggcgccgcg
      181 tcggcacgag ctcgtcctgc tgaacgggat cgggatcgat ctggcccatt tcgcctacgc
      241 gccgccgccg gtcgagcggc cgagctttct cctggtcggc cggctgatca aggacaaggg
      301 ggtggtcgcc tatgccgacg cggcacgccg ggtcaaggcc gagttcccga aagcccgctt
      361 cgcgctcctg ggcagcttcg acagctcccc cggtgccctg gcgcccgacc aggtcgaggc
      421 gtggcaacgc gagggcatca tcgactacct cggtaccgtc cacgatgtcc gaccggtcat
      481 cgcggacgcc gatgtcttcg tcttgccgtc cacctaccgg gagggcgtgc cgcgctcgac
      541 cctcgaggcg atggccatgg gccggccgat catcaccacc gacgcgcccg gctgccgcga
      601 gaccgtccgc cacggcgaga acggctatct ggtcgccaag cacgatgtcg aggggctcgc
      661 tgtcgccatg cgccgcttcg cggcggcgcc cgagctggcg ctgccgatgg gccttgccag
      721 ccggcggttg gtcgaggcgc gcttcgacga ccgtgaggtc aacgacacca ttctcgaaac
      781 catgcggcta tgaggccgac cgtcgggagt cgagcggcca gggcctcagc cgcgataggc
      841 cgggaagaag cgggtcgccg gccggccggc ggtcagcgct tcataggccg gcgcatcgat
      901 gatccagcgc cagacccgga aggcgtgacg gcgcggcgag aagcccgcct tgaagtggaa
      961 gagcgaatcc tcgatcgcac ctcgtccgcc gcccagatgc aggaagtccg ccccggcggc
     1021 ccggaaatgt cgtcggctcg cgtcgatcag ctgcttggca ggtgctgctc cggcgtgacg
     1081 gtcggagacg gcggccagat gatattgcac gatgggtccg tgccgcatga acaggccagc
     1141 tccgacgatc tctccgtcga gatcgcagag ggcgagagtg agggccggtc cgagggtctc
     1201 ggccagccgc tcgaaatggg cggcatcgaa gaagtagcgc gactgcgcct gcacgcgccg
     1261 catgttctcg taatagagct cgacgaaggg cccgaggtca cccggccgct cgttcaccat
     1321 ggtgcggtac cccttgcgct cgagcttcgc gatgtcgcgc cgatggttgc tgcgataggt
     1381 ggcgagctgc gcttcctcgg gcagccgaag gtcgatcgag acggtgtcgc ccaggcgcgc
     1441 gagctcaccc atgcccgcga ggaccggcgc ctgctcgagc agcgggtgga ggcgggcgaa
     1501 gagggtgacc gcaccgcgct cgacgagacc ctggtgcagc gttcggtgga agtcggccac
     1561 gaggtcctga ccgaccggcg ccgtggaggt gatcggcccg gcatagccgt agaccgacgt
     1621 cgcgtcgcag cgccccggcg caccttcgat cggctgcagc agcagcggca aggcgatcca
     1681 gcgccgaccg tcgtccacga cgtgcagcca cgcctcgccc cagccggaag cctcggcgac
     1741 ccggtgatag cccggcaggt gatagacgtc gtgcggaccg gccgaggcga gcatgtcagc
     1801 ccacccggca tcgtcgctcc gcagccaccg tgcaccgcgc attcgggaga cctcgacccc
     1861 aggcgcccga tgcgcctccc tgtagctcta ccaaggggcg caatctgatg gaagcgatca
     1921 acccgccgcg gaccctctat gtacgctacg gcaaacgcct cttggacctg gcgatcgccg
     1981 tgccgctggc cctgctcctc tcgcccttga tgctggtggt cgccatcgtc gtgcgctggc
     2041 gcctgggtgc cccggtgctg tttcgccagg atcgcatcgg caaggacgac cggcacttcc
     2101 gtctcgccaa gttccgcacc atgaccgacc ggcgcgacgc ctcgggtgcc ctcctgccgg
     2161 acggcgaccg gctgacgccg ctcgggctct ttctgcgcaa gaccagcctc gacgagctgc
     2221 cgcagctctg gaacgtgctc agaggcgaca tgagcctgat cggcccgcgg ccgctgctgg
     2281 tcgagtatct gccgcgctac aacgcccgcc agcgctgccg ccacaaggtc atgcccggga
     2341 tcaccgggct cgcccaggtg gccgggcgca acctgctctc ctgggaggcg cgcttcgagc
     2401 acgacgtcac ctatgtgcgc gagatcggcc cggccctcga ttgcaggt
//
LOCUS       DAOTFI010000403         2407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_52141_length_2407_cov_7.139881, whole genome shotgun sequence.
ACCESSION   DAOTFI010000403 DAOTFI010000000
VERSION     DAOTFI010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52141_length_2407_cov_7.139881"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="P5558_23750"
     CDS             <1..803
                     /locus_tag="P5558_23750"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRY27399.1"
                     /translation="LRALGIEVVHDLPGVGRNFHDHIDAYVISELSGPHSYDRHTRPH
                     RMLMAGLEYQLFGKGPVTSNLAEGGAFVLTDPAEPSPDIQHHFLPGAGLEAGVPPVPS
                     GYGCTLNACYLRPKSRGSIRLRSADPARAPVIDPNYWAEPEDMAAALRGIASSREIMA
                     QAAFRPFVKGEHLPGPAVASRADLEAYARRYGKTDYHPVGSCKMGRDERAVVDPDCRV
                     HGIERLRICDSSIMPRVVSSNTNAASIMIGEKASDLIKGNRRTAEVVA"
     gene            905..1297
                     /locus_tag="P5558_23755"
     CDS             905..1297
                     /locus_tag="P5558_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27400.1"
                     /translation="MSTRLDFDDGSKRRTPVALVLAAVVGAVFGALATGIVGVGLGVF
                     SSNAAGAVSGGAMIGAAFIFAVCLIWNLEIRARGQRLNRVLEVELADDLQENQAALRR
                     LDSLLLLARRGGRAASQHTGGRDRSGPS"
     gene            1843..>2407
                     /locus_tag="P5558_23760"
     CDS             1843..>2407
                     /locus_tag="P5558_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY27401.1"
                     /translation="MPMKPVKWGMLGVARIGMEKVIPAMQQSDLCEIVGVASRSAEKA
                     EAGRAQLGIAKAYGSYEDLLADPEIEAVYNPLPNHLHVPWTVKALEAGKHVLCEKPLG
                     MDAAKAATLVAARDRSGRLVQEAFMVKCHPQWLKAKALIDEGRIGEPRAASGIFCYHN
                     TDPENVRNMADIGGGGIMDIGCYMTIFS"
BASE COUNT          409 a          808 c          806 g          384 t
ORIGIN      
        1 agctccgtgc cctaggcatc gaggtggtcc acgacctgcc gggcgtcggc cgcaacttcc
       61 acgaccatat cgacgcctat gtcatcagcg agctctccgg tccccactcc tacgatcggc
      121 acacgcggcc gcaccggatg ctgatggccg ggctcgagta ccagctcttc ggcaaggggc
      181 cggtgacctc caacctcgcc gagggcgggg ccttcgtgct gaccgacccc gccgagccgt
      241 cgcccgacat ccagcaccat ttcctgcccg gcgccggcct cgaggccggc gtgccgccgg
      301 tgccgtccgg ctatggctgc acgctcaacg cctgctacct ccggccgaaa tcccggggca
      361 gcatccggct ccgctccgcc gacccggcca gggccccggt catcgacccc aactactggg
      421 ccgagcccga agacatggcg gcggcgctcc gcggcatcgc ctcgtccagg gagatcatgg
      481 cgcaggccgc cttccggccg ttcgtcaagg gcgagcatct gcccggcccc gcagtcgcct
      541 cgcgcgccga tctcgaggcc tacgcccgcc gctacggcaa gaccgactac catccggtag
      601 gctcctgcaa gatgggcagg gacgagcgag ccgtggtcga ccccgactgc cgcgtgcacg
      661 gcatcgagcg gctgcgcatc tgcgacagct cgatcatgcc ccgcgtcgtc agctccaaca
      721 ccaacgccgc ctcgatcatg atcggcgaga aggcgtccga cctgatcaag ggcaaccgcc
      781 ggacggccga ggtggtggcg tagcgccctc tctcgatttg agcctctcgt aatgttatat
      841 tcagccaacg gctgtccggt gccggaattc ttcgacgccg gtggcaactc gggattggta
      901 tggcatgtcg acccggctgg atttcgacga cggcagcaag cgacgaacac cggtggcact
      961 cgtcctcgcc gccgtggtcg gcgcggtctt cggcgcgctg gccaccggca tcgtgggtgt
     1021 ggggctgggt gtcttttcgt ccaacgccgc gggtgccgtc tcgggcggag ccatgattgg
     1081 tgccgccttc atattcgccg tgtgcctcat ttggaacctg gagatcagag caagaggcca
     1141 gcgcctgaac cgcgtccttg aggtcgagtt ggcggacgat ttgcaggaga atcaggcagc
     1201 tctccgtcgg ttggactcgc tgctgctcct ggcccgccgg ggaggccgcg ctgcgtcgca
     1261 gcatacaggc gggcgcgacc gatccggacc atcctgacct gccaccgttg gatcgcaacg
     1321 agcttgttcg agagctcgag agctgccggc aagcaatcga ggagatcgac gagcggagga
     1381 agcggctgga agccaggcgg ctatcggcgg gcgcgaagct ccgggtgccg atgggcaagc
     1441 tcgtccggga tgtcatcggc gcgagcgccg gcattgccct ggcaccgccg acgtccggcc
     1501 tgagtctcct cgtgctccct gatcgttgac gggctggaca tatcgagcga tgtcgccgac
     1561 ctctatggcg tggcgatcga ggcgcggcag cttcgatttc gacgacagtc gacgatgctt
     1621 cgggtggacc agatcgaacg gatcttgcgc ggtgccgact gattcgctcg acccgagcta
     1681 cgtccaaact ggacgacagg ctaacggtcg aagaggcggc cgggccggac gatcagccaa
     1741 ggatcgaacg cctcgcgttt gcgatggcag cctcaaggga cggacgcaac cgcgtgccga
     1801 tctcgtgcgg cgaacaccaa gcaacatcat gcaggaggaa ccatgccaat gaagcccgtc
     1861 aaatggggga tgctcggcgt cgccaggatc ggcatggaga aggtcatccc ggcgatgcag
     1921 cagagcgatc tctgcgagat cgtcggtgtc gcctcgcgct cggccgagaa ggccgaagcc
     1981 gggcgggcgc agctcggcat cgccaaagcc tatggctcct acgaggatct cctcgccgac
     2041 ccggagatcg aggcggtcta caaccccttg cccaaccatc tgcacgtgcc ctggacggtc
     2101 aaggcgctcg aggccggcaa gcatgtgctc tgcgagaagc cgctcggcat ggacgcggcc
     2161 aaggcggcga cgctggtggc ggcgcgcgac cggagcggca ggctcgtcca ggaggccttc
     2221 atggtgaagt gccacccgca gtggctgaag gccaaggcgc tgatcgacga gggccggatc
     2281 ggcgagccgc gggcggcctc gggcatcttc tgctaccaca acaccgatcc ggagaacgtc
     2341 cgcaacatgg cggatatcgg cggtggcggg atcatggata tcggctgcta catgacgatc
     2401 ttctcgc
//
LOCUS       DAOTFI010000404         2382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_52880_length_2382_cov_5.033949, whole genome shotgun sequence.
ACCESSION   DAOTFI010000404 DAOTFI010000000
VERSION     DAOTFI010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52880_length_2382_cov_5.033949"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="P5558_23765"
     CDS             complement(<1..628)
                     /locus_tag="P5558_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27402.1"
                     /translation="MSFLLKRSLVLLCSATLVVLVAACGSDVVAIPPTPADVAECPGD
                     WALMVSCEGTADKVPVVLDRNPGAKTGAATGAMASVALGTGIAVGLLKGAGEAADEGG
                     GLGAAFGGAMIVLAGVAFVAGVVLAPVGAVVGAGIGAGTAEPDAAAQAAHDSLVRAVE
                     QTRLDRDFRERLVAAAEDVAGRTLFDCGALDVPAACIEASPEPIGQVLS"
     gene            842..1987
                     /locus_tag="P5558_23770"
     CDS             842..1987
                     /locus_tag="P5558_23770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27403.1"
                     /translation="MRQWTPITVAIAIALTATAAARGSESPPLATLARLDPSSVTVSG
                     ISSGGFFAHQFHVAFSSLVEGAGIIAGGPYACAEQIPWALGFNPLARVTIALGVCTRY
                     ARGAFDPFDLWLPDAPDAEEAIAAVRAEHAADRIDDPANLADDRVWLFLGDADGIVPL
                     ATMQALEGVYAGLGLTDPALEFERNAEANHGLPIEEFTGTTEHTKLGCGDYGPPFLID
                     CDFDAAERLLRHLYPAGFAEEPGIPDPDRLAAFDQSEFFDADDPSVSLAATGFLYVPE
                     TCRDDAPPGEPCRLHVAFHGCQQYAGLIGDDFYMDGGYNRWAEANRIVVLYPQTTPWE
                     RALDPTGLTANPKGCWDWWGYSGPDYFRREGQQMQAVHGMIERLLPQ"
     gene            complement(2004..>2382)
                     /locus_tag="P5558_23775"
     CDS             complement(2004..>2382)
                     /locus_tag="P5558_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HRY27404.1"
                     /translation="LKPWSVIGLLATVVLLFGFQGEMILAQPLLILLIAAPLLIQSYA
                     IFLVAYAAAWAWRVPHDVAAPCALIGTSNFFELAVAVAISLFGLQSGAALATVVGVLV
                     EVPVMLSLVAFANRTRDSFPASG"
BASE COUNT          408 a          903 c          739 g          332 t
ORIGIN      
        1 cactcaggac ctgcccgatc ggctccggcg aagcctcgat gcaggcggcc ggcacatcca
       61 gggccccgca atcgaacaag gtccggccgg cgacgtcttc ggcagccgcg acgagccgct
      121 cgcggaagtc cctgtcgagc ctggtttgct cgaccgcccg cacgagactg tcatgcgctg
      181 cctgcgccgc ggcgtccggc tccgccgtcc cggcgccgat cccggcaccg actaccgcac
      241 cgaccggcgc cagcacgacc ccggcgacga acgccacgcc ggcaagaacg atcatggcgc
      301 ccccgaacgc cgcaccgagg ccgcccccct catcggcggc ttcgccggct cccttcagga
      361 ggccgacagc aatgcccgtg cctagggcga cgctggccat cgcccctgtg gcggcaccgg
      421 tcttggctcc ggggttgcga tccaggacga caggcacttt gtccgccgta ccttcgcagc
      481 tgaccatgag cgcccagtct ccaggacatt cagcgacatc tgcgggtgtc ggcgggatcg
      541 ccaccacatc ggatccgcac gccgcgacca ataccacgag cgtggcactg caaagaagaa
      601 caagactgcg tttcagcagg aaagacatgt actacaactc ccccgatatt tcggactggc
      661 aggatcagct agggtcagca ttttgcttag ctggcatcgg gaggatttcc ttgacttata
      721 tcaacgacac atcaaccccc aagccgacga cagtagggtc agttctcccg tcattgccat
      781 tcccgtcgcc ggcacgaacg tgctagcgcg atgtggcgaa gcgccggcgc tggagatgcc
      841 catgcgacag tggacgccca tcacggtggc aatcgccatc gccctgaccg ccacggcagc
      901 ggcgcgcggc agcgaatcgc cgcccctcgc caccctggcc cgcctcgacc cgtccagcgt
      961 caccgtctcc ggcatctcct ccggcggctt cttcgcccac cagttccacg tcgccttctc
     1021 gtcgctcgtc gagggcgccg gcatcatcgc gggcggcccc tatgcctgcg ccgagcagat
     1081 cccctgggcc ctcggcttca acccgctggc gcgggtgacc atcgcactcg gggtctgcac
     1141 ccgctacgcc cgcggggctt tcgatccctt cgacctgtgg ctgccggacg cccccgatgc
     1201 cgaagaggcg atcgccgccg tgcgggccga gcacgcggcg gaccggatcg acgacccggc
     1261 caacctcgcc gacgatcgcg tctggctctt cctgggcgac gccgacggca tcgtgccgct
     1321 cgcgaccatg caggcgctgg aaggggtcta tgccggcctc ggtctcaccg acccggcgct
     1381 cgagttcgag cgcaacgccg aggccaacca cggcctgccg atcgaggaat tcaccggcac
     1441 gaccgagcat acgaagctgg gctgcggcga ttacgggccg cccttcctca tcgactgcga
     1501 cttcgatgcc gccgagcgcc tgctccgcca tctctacccc gccggattcg cggaggagcc
     1561 tggaataccc gaccccgacc gcctcgcggc cttcgaccag agcgagttct tcgacgccga
     1621 cgaccccagc gtcagcctgg cggcgaccgg cttcctctac gtccccgaga cctgccgcga
     1681 cgacgcgccg ccgggcgagc cctgccggct gcacgtcgcc ttccacggct gccagcaata
     1741 tgccggcctg atcggcgacg atttctacat ggacggcggc tacaaccgct gggccgaggc
     1801 caaccgcatc gtcgtgctct acccccagac gacgccctgg gagcgcgccc tcgaccccac
     1861 cggcctcacc gccaacccca agggctgctg ggactggtgg ggctatagcg gccccgacta
     1921 cttccgccgg gaaggccagc agatgcaggc cgtccatggc atgatcgaac ggctcctgcc
     1981 gcagtgagcg cgggcgcccg ggctcaaccg gacgccggaa agctgtcccg cgtgcgattg
     2041 gcgaaggcca ccagcgacag catcaccggc acctcgacca gcacaccgac caccgtggcg
     2101 agcgccgccc ccgactgcag cccgaagagg ctgatcgcca ccgccaccgc cagctcgaag
     2161 aagttcgagg tgccgatgag cgcacagggt gcggccacgt cgtgcggcac ccgccacgcc
     2221 caggcggcgg cataggcgac gaggaagatg gcataggact ggatcaagag cggcgcggcg
     2281 atcaggagga tcaggagcgg ctgcgccagg atcatctcgc cctggaagcc gaagagcagg
     2341 acgacggtgg ccaagagccc gatcaccgac catggcttga gt
//
LOCUS       DAOTFI010000405         2354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_53705_length_2354_cov_5.963897, whole genome shotgun sequence.
ACCESSION   DAOTFI010000405 DAOTFI010000000
VERSION     DAOTFI010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53705_length_2354_cov_5.963897"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="P5558_23780"
     CDS             <1..513
                     /locus_tag="P5558_23780"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRY27405.1"
                     /translation="YHDEDLDLGNGRHLIEPLVLGRMLQVSRPRPDEVALVVGCDTGY
                     AAAVLSKLVATVFLLAPDAEAAAAIEAVLDELDCNNVVVQPGRAADGLPAQAPFNLIL
                     LAGSVPALPAALVAQLEDRGRMVGVVQKGRFGKLTLLTRVGDAHGTTAPFDAWLPPVA
                     GLQPAESFEF"
     gene            592..2055
                     /locus_tag="P5558_23785"
     CDS             592..2055
                     /locus_tag="P5558_23785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRY27406.1"
                     /translation="MRPSRVTRARLIALLLALTPASVSAAVTLPTALSKAYLENPTLV
                     AARARLRAADEGVPIARASRRPQIAATTGASVGLQESNRGSRSTSSFRQALGIEQPLY
                     TGGEASARIGRAETLVEAERARLEAVEQAVLLAAIEAFTAVLQAQRVLELALGNETRL
                     GRQLEAVQRRYRFGELTQTDVAQAEARYARGIADREAAAGELETAGAAYRKVIGDPPG
                     TLVEPVMPADLPPDEQAALERIADNPNVRAADFGVGVARREVDVALSALKPRLKLRGE
                     ASYVDEPSGRYDWQSDVSIGAVLSIPLYQGGGEYGRIRQTRQLLTEGRYTLESARRDV
                     REDIQAAWQDLQTARARGRSLARQVESAALALDGVRQESLVGARTVLDVLDAEQELFD
                     AEVAAAAAARDSIVAAYRLKAGMGELTAMALDLDAVPYDPDAYYQEVRSAWFGTGDSA
                     ADDPPGPETDDDQDGAVADDADAEGETMDTEAMPEGG"
     gene            2166..>2354
                     /locus_tag="P5558_23790"
     CDS             2166..>2354
                     /locus_tag="P5558_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HRY27407.1"
                     /translation="MSTDKNDQEPSMEEILSSIRRIIADDQDEEGGGAKPGADPAKAK
                     AAKPTQSHAEEDVLDLTQV"
BASE COUNT          381 a          800 c          838 g          335 t
ORIGIN      
        1 taccatgacg aggatctcga cctcggcaat gggcggcacc tgatcgagcc tctggtcctc
       61 gggcggatgc tgcaggtgag ccgtccccgc cccgacgagg tggcgctcgt cgtcggttgc
      121 gataccggct atgcggcggc ggtcctctcg aagctggtgg ccacggtctt cctgctcgcc
      181 cccgatgccg aggccgcggc ggcaatcgag gccgtgctcg acgaactcga ctgcaacaac
      241 gtggtcgtcc agccgggccg ggcagccgac ggcctgccgg cccaggcgcc gttcaatctc
      301 attcttctcg ccggctcggt gccggccctg cccgcagcct tggtcgcaca gctcgaagat
      361 cggggccgca tggtcggcgt ggtgcagaag ggtcgcttcg gcaagctcac gctgctcacc
      421 cgggtcggcg acgcgcatgg caccacggca cccttcgacg cctggctgcc gcccgtggca
      481 ggattgcagc cggcggaatc cttcgagttc tgacagatga attatgatcc gaatggttga
      541 acaagaccat tcgttctagc gaaaccggtt aaaccgtgct agcgatcggg catgcgtccc
      601 agtcgtgtga cccgtgcccg gttgatcgcc ctgctgctgg ccctcactcc cgccagcgtc
      661 tcggcggccg tcaccctgcc gacggcgctg agcaaggcct atctcgagaa ccccacgctc
      721 gtcgccgccc gtgcccggct gcgcgctgcc gacgagggcg tgccgattgc ccgggccagt
      781 cggcggccgc agatcgccgc gaccaccggg gcatcggtcg gcctgcagga atcgaaccgc
      841 ggcagccgct ccacctcgag ctttcgccag gcgctcggca tcgagcagcc gctctatacc
      901 ggtggcgagg cgagcgcccg gatcggccgg gccgagactc tcgtcgaggc cgaacgggca
      961 aggctcgagg cggtcgagca ggcggtcctg ctggccgcga tcgaggcttt cactgcagtg
     1021 ctccaggccc agcgcgtgct ggagctggcg ctcggcaacg agacccggct cggccgccag
     1081 ctcgaggcgg tgcagcgccg ctaccgcttc ggcgagctga cccagacgga tgtcgcccag
     1141 gccgaggccc gctatgcccg gggcatcgcc gaccgggaag ccgccgccgg cgagctggaa
     1201 acggccggcg ccgcctatcg caaggtcatt ggtgatccgc ccgggacgct ggtcgagccg
     1261 gtcatgccgg ccgatcttcc cccggacgag caggccgccc tggagcggat cgcggacaac
     1321 ccgaacgtgc gcgccgccga cttcggcgtc ggcgtggcgc gccgcgaggt ggacgtggcg
     1381 ctttcggcgc tcaagccgcg cctgaagctg cggggcgagg ccagctatgt cgacgagccg
     1441 agcggtcgct acgactggca gagcgacgtc tcgattggcg ccgtcctctc gattccgctc
     1501 tatcagggcg gcggtgagta cggacgtata cgccagacgc ggcagctgtt gacagagggg
     1561 cgctacacgc tcgaaagcgc tcgtcgtgac gttcgcgagg acatccaggc ggcctggcag
     1621 gatctgcaga cggcccgcgc ccgcggccgg tcgctcgccc gccaggtcga gtcggcggcg
     1681 cttgcgctgg acggcgtgcg gcaggaatcg ctggtgggtg cccggaccgt cctggacgtg
     1741 ctcgacgccg agcaagagct gttcgacgcc gaggtggcgg cagcagcggc ggcgcgcgac
     1801 agcatcgtcg ctgcctatcg gctcaaggcc ggcatgggcg agctcaccgc catggcgctc
     1861 gacctcgacg ccgttcccta cgacccggat gcctactacc aggaggtgcg cagcgcctgg
     1921 ttcggcaccg gcgacagtgc cgcggacgac ccgccggggc cggagaccga cgacgatcag
     1981 gatggggcag tggcggacga tgcagatgcg gaaggcgaaa cgatggacac ggaggcgatg
     2041 ccagaaggcg gctaggagcc tgtccgggaa agggtcctag ggttcaggca aaattcacca
     2101 tttgccccgc ataagggcgg gcgtgtcagc gacggcgaca acggcagcag atcgagttga
     2161 atggcatgag caccgacaag aacgaccagg agccgtcgat ggaggagatc ctctcctcga
     2221 tccgacggat catcgccgac gaccaggacg aggaaggcgg cggcgcgaag ccgggggccg
     2281 atccggccaa agcgaaggcg gcgaagccga cccagagcca cgccgaggag gatgtcctgg
     2341 atctcaccca ggtc
//
LOCUS       DAOTFI010000406         2330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_54463_length_2330_cov_6.636484, whole genome shotgun sequence.
ACCESSION   DAOTFI010000406 DAOTFI010000000
VERSION     DAOTFI010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54463_length_2330_cov_6.636484"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..865
                     /locus_tag="P5558_23795"
     CDS             170..865
                     /locus_tag="P5558_23795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRY27408.1"
                     /translation="MRRVLVVQHHADSPLGVMAPVLRAADVELDVLQAEHGCDWPRDA
                     MAADGLVVLGGAMSANDDARCPHYGALLETIRQFTAAGRPVLGICLGGQLIARALGGR
                     VVEGQRGEFGFVELRGRPAAHDDPLLGGVRLPVPVMQWHEDHFELPPGAVHLLASDAC
                     PAQAFRAGRASWGFQCHLEVDAPSLQAWAALRARLTGDPAVPDAVAAAAERHHATAMA
                     FGRTVAERWLALV"
     gene            complement(862..1596)
                     /gene="pdeM"
                     /locus_tag="P5558_23800"
     CDS             complement(862..1596)
                     /gene="pdeM"
                     /locus_tag="P5558_23800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HRY27409.1"
                     /translation="MTATRPLTLDHGGEVVELDPLGAVHLPAHRCLVVADLHLEKGSA
                     YARLGTFLPPYDTARTLRRLDWAIQRYRPRLVVSLGDAFHDTAGPGALDPEARRTLEA
                     LARGRSWLWVRGNHDPHPPAGLAGETIDAADLGRLHLTHVPPTAPAGRIILAGHLHPK
                     ARLAQARPRSRQCATRPCFAGNPDLLLLPAFGAYTGGLNVLDPAIAGHFARGFSAFLL
                     GDERVFQVAHRHLTPDPPDWRRHRIA"
     gene            complement(1593..>2330)
                     /locus_tag="P5558_23805"
     CDS             complement(1593..>2330)
                     /locus_tag="P5558_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HRY27410.1"
                     /translation="LAEPAHWPALPAPVREWLELQRRHSALVAADELLVESFWRAGRH
                     FLVAYSFVGRNAHQTLGMLLTRRLQRMGARPIGFVASDYCIATWSLGECHDIEALFDV
                     DMLGDDLEEWIAESSMVKRIFRNCAVVAGLIERRHPGQEKTGRQVTFNADLIYDVLRK
                     HEPDHVLLKAARAEAAAGLTDIRRLADLLTSVQGRIRHRRLDRISPFAVSIVAGIGKE
                     RVDAGDLDQTLDELTAELVAEALAEPA"
BASE COUNT          301 a          743 c          960 g          326 t
ORIGIN      
        1 cccagatcga tgacgtactc gatcttctcg cgcggctcgt cgaagagggc gaactcgtcc
       61 tcgatggcac gacacgtggc ggcgaagtct tgctggtgct gctcggtcat gcccaccata
      121 tgcgtggccg gccgggacgg gtcaacgcga gcgcgggaag agagatcgca tgcgacgggt
      181 gctcgtggtg cagcatcatg ccgattcgcc gctgggcgtc atggcgcccg tgctgcgcgc
      241 cgcggacgtg gagctcgacg tgctccaggc cgagcacggc tgcgactggc ccagggatgc
      301 catggcggca gacggcctcg tcgtgctcgg cggggcgatg agcgccaatg acgacgcacg
      361 ctgcccgcac tacggcgcgc tgctcgagac gatccggcag ttcacggcgg cagggaggcc
      421 ggtgctcggc atctgcctcg gcggccagct catcgcccgg gccctcggcg gtcgggtggt
      481 cgaggggcaa cgcggcgagt tcggcttcgt cgagctcagg ggccgccccg ccgcgcatga
      541 cgacccgctg ctcggcgggg tccggctgcc ggtgccggtc atgcagtggc acgaggatca
      601 tttcgagctg ccgccgggtg ccgtgcatct cctcgccagc gacgcctgcc cggcccaggc
      661 ctttcgggcc ggccgggcga gctggggctt ccagtgccat ctcgaggtgg acgcgccgag
      721 cctccaggca tgggcagcgc tgcgcgcccg gctgaccggc gatcccgccg ttcccgatgc
      781 ggtggcagcg gcggcggagc ggcaccacgc caccgccatg gccttcggtc ggacggtggc
      841 cgaacgctgg ctggcgctcg tctaggcgat ccggtggcgg cgccagtcgg gcgggtcggg
      901 ggtgaggtgg cggtgggcga cctggaagac ccgctcgtcg ccgaggagga aggcgctgaa
      961 gccgcgagcg aagtggccgg cgatggcggg gtcgaggacg ttgaggccgc ccgtgtaggc
     1021 gccgaaggcg ggcagcagca ggaggtccgg gttgccggcg aagcagggcc gggtggcgca
     1081 ttgccggctc cgggggcggg cctgggcgag ccgcgccttg gggtggaggt ggccggcaag
     1141 gatgatgcgc ccggcgggcg ctgtgggcgg gacgtgggtc aggtgcagcc ggccgagatc
     1201 ggccgcgtcg atcgtctcgc cggcgaggcc ggccggcggg tgcgggtcgt ggttgccgcg
     1261 cacccagagc cagctccggc cccgggcgag ggcttcgagc gtgcggcggg cctcggggtc
     1321 gagggcgccc gggccagccg tgtcgtggaa ggcatcgccg aggctgacca ccaggcgcgg
     1381 ccggtagcgc tggatcgccc agtcgaggcg gcggagcgtg cgggcggtgt cgtagggcgg
     1441 caggaaggtg ccgagcctcg cataggcgct gcccttctcc agatggaggt cggcgacgac
     1501 gaggcagcgg tgggcgggaa ggtgcacggc gccgagcggg tcgagctcga ccacctcgcc
     1561 gccgtggtcg agggtgagcg gcctcgtggc ggtcatgcgg gctcggccag cgcttcggcg
     1621 accagctcgg cggtcagctc gtcgagggtc tggtcgagat cgccggcgtc gacgcgctcc
     1681 ttgccgatgc cggcgacaat cgagacggcg aagggcgaga tgcggtcgag ccgccggtgg
     1741 cggatgcggc cctggacgga tgtgaggagg tccgcgaggc ggcggatgtc ggtgagcccg
     1801 gccgcggcct cggcccgggc ggccttcagg agcacgtggt cgggctcgtg cttgcgcagc
     1861 acgtcgtaga tcaggtcggc gttgaaggtg acctggcggc cggtcttctc ctgccccggg
     1921 tggcggcgct cgatcagtcc ggcgacgaca gcgcagttgc ggaagatgcg cttgaccatg
     1981 ctgctctcgg cgatccactc ctcgaggtcg tcgcccagca tgtcgacgtc gaagagggcc
     2041 tcgatgtcgt ggcattcgcc caggctccag gtggcgatgc agtagtccga ggcgacgaag
     2101 ccgatgggcc tggcccccat tcgctgcagg cggcgggtga gcagcatgcc gagcgtctgg
     2161 tgggcgttgc ggccgacgaa gctgtaggcc acgaggaaat gccggccggc ccgccagaag
     2221 ctctcgacga gcagctcgtc ggcggcgacg agggcggaat ggcggcgctg cagctcgagc
     2281 cattcgcgca ccggtgccgg gagagccggc cagtgggcgg gctcggccag
//
LOCUS       DAOTFI010000407         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_54680_length_2323_cov_8.921517, whole genome shotgun sequence.
ACCESSION   DAOTFI010000407 DAOTFI010000000
VERSION     DAOTFI010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54680_length_2323_cov_8.921517"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /gene="purL"
                     /locus_tag="P5558_23810"
     CDS             <1..1491
                     /gene="purL"
                     /locus_tag="P5558_23810"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRY27411.1"
                     /translation="QVGDPFTEKLLIEACLELMAGDCIVAIQDMGAAGLTSSSFEMAS
                     RGQCGILMNLDAVPLRETGMTPYEIMLSESQERMLMVVAAGREGEAVAVFEKWGLDCA
                     VVGRVTDTGRMVLTWQGLTVADVPVAPVAGASPVLDRPYELTTPPVPVASPANLDAGP
                     VDRLLLALMGSPDIASKRWIFEQYDHMVMADTVMLPGRGDAAVVRVHGTGRGLALTTD
                     CTPRYCEADPFMGGMQAVAEAWRNLSATGARPLAITNCLNFGSPERPRIMGQLVGCIE
                     GMAEACRALDFPVVSGNVSLYNETDGRAILPTPNVGGVGLVDDTGRMAGLAWDVAGLE
                     LVLVGETQGWLGCSLLWQQFLGRDDGAPPPVRLEIERHNGDFVRAAIGAGQVRAVHDL
                     SDGGLAVALAEMCLASAMGATIAMPHDTPSVLGWLFGEDQARYLLAVPKAGLDPLLQE
                     AGEAGVVARAIGVTGGDALTLGDAAAISLADLRARHEGWLPNLMAG"
     gene            1528..1761
                     /locus_tag="P5558_23815"
     CDS             1528..1761
                     /locus_tag="P5558_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HRY27412.1"
                     /translation="MPMSATEIEAAIRTALPDAEISIEDLAGDGDHYRATVVSSSFKG
                     MPRVRQHQLVYDALGGRMGGTLHALALTTKTPA"
     gene            1797..2126
                     /gene="grxD"
                     /locus_tag="P5558_23820"
     CDS             1797..2126
                     /gene="grxD"
                     /locus_tag="P5558_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRY27413.1"
                     /translation="MTDPIHATIQDQITKDDVVLYMKGTPVFPMCGFSAGTVQILGQL
                     DVKFQAYNVLDDQNLREGIKTFSNWPTIPQLYVKGEFIGGFDIVREMAENGELQETLK
                     EKGVAAA"
BASE COUNT          368 a          832 c          777 g          346 t
ORIGIN      
        1 caggtgggcg accccttcac cgagaagctg ctgatcgagg cctgcctcga gctgatggcc
       61 ggcgactgca tcgtcgccat ccaggacatg ggggcggccg ggctcacctc ctcatcgttc
      121 gagatggcgt cgcgcgggca atgcggcatc ctgatgaacc tcgacgcagt gccgctgcgc
      181 gagaccggca tgacgcctta cgagatcatg ctctcggaga gccaggagcg catgctgatg
      241 gtggtggccg ccggccgcga gggcgaggcc gtggccgtct tcgagaaatg ggggctcgac
      301 tgcgccgtcg tcggccgcgt caccgacacc ggccggatgg tgctgacctg gcagggcctg
      361 accgtggccg acgtgccggt ggccccggtc gccggcgcct cgccggtgct cgaccggcct
      421 tatgagctga ccacgccgcc cgtgccggtg gcgagcccgg ctaacctcga cgccggcccg
      481 gtcgaccggc tcctgctggc gctcatgggc tcgcccgata tcgccagcaa gcgctggatc
      541 ttcgagcagt acgaccacat ggtgatggcc gacacggtga tgctgccggg ccggggcgac
      601 gccgcggtgg tgcgggtcca cggcaccggc cggggcctgg cgctgaccac cgactgcacg
      661 ccgcgctatt gcgaggccga ccccttcatg ggtggcatgc aggccgtggc cgaggcctgg
      721 cgcaacctct cggccaccgg tgcccggccg ctcgccatca ccaactgcct gaacttcggc
      781 agccccgagc ggccgcgcat catgggccag ctcgtcggct gcatcgaagg catggccgag
      841 gcctgccgtg ctctcgactt ccccgtcgtc tcgggcaatg tctcgctcta caacgagacc
      901 gacggccgag cgatcctccc cacccccaat gtcggcggcg tcggcctggt cgacgacacc
      961 ggccgcatgg ccgggctcgc ctgggacgtg gccggcctcg agctcgtgct ggtcggcgag
     1021 acccagggct ggctcggctg ctcgctgctc tggcagcagt ttctgggacg tgacgacggc
     1081 gcaccgccgc cggtccgcct cgagatcgaa cgccacaacg gcgatttcgt ccgggcggcg
     1141 atcggcgccg gccaggtccg ggccgtgcac gacctctcgg atggcgggct cgccgtggcg
     1201 ctcgccgaga tgtgcctcgc cagtgccatg ggtgccacga tcgccatgcc gcacgatacg
     1261 ccctcggtgc tgggctggct cttcggcgag gaccaggccc gctacctgct ggccgtgccg
     1321 aaggccgggc tcgaccccct cctgcaggag gctggcgagg ccggcgtcgt cgcccgcgcc
     1381 atcggtgtca ccggcggcga cgcgttgacg ctcggcgacg cagccgccat atccttggcg
     1441 gacttgcgag cccggcacga gggctggctg ccgaacctga tggccggcta ggccgccagc
     1501 ccgagtgaat tcgacgagga tgcccgaatg ccgatgagcg cgaccgaaat cgaagcagcg
     1561 atcaggaccg ccctgcccga tgccgagatc agcatcgagg atctcgccgg cgacggcgac
     1621 cactatcgcg ccacggtcgt ctcgagcagc ttcaagggca tgccccgggt ccgccagcac
     1681 cagctcgtct acgacgcgct cggcggccgc atgggcggca ccctgcacgc cttggccctg
     1741 accaccaaga cacccgcctg acgcccggcc gatcgcggcc ccaaggagac cattcgatga
     1801 ccgacccgat ccacgccacc atccaggacc agatcaccaa ggacgacgtc gtcctctaca
     1861 tgaagggcac gcccgtcttc ccgatgtgcg gcttctcggc cggcaccgtg cagatcctgg
     1921 gccagctcga cgtcaagttc caggcctaca acgtcctcga cgaccagaac ctgcgcgagg
     1981 gcatcaagac cttctccaac tggccgacca tcccccagct ctacgtcaag ggcgagttca
     2041 tcggcggctt cgacatcgtc cgcgagatgg ccgagaacgg cgagctgcag gagacgctga
     2101 aggagaaggg cgtggcggcg gcctaaacga tagcacaggt aaaaagacgg ggaggatgat
     2161 cctccccgtg ccccaccggt ttttgtacgc ctccgtcgtg cagaaagacg gaaggggccc
     2221 ggggaaactc agtttccccg aacattctac ccgtcttcct cgctgaccag ctcgatcgag
     2281 ctctcgatcg cggaatccct ggagcggatg agcagcggca tcg
//
LOCUS       DAOTFI010000408         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_55509_length_2298_cov_6.194828, whole genome shotgun sequence.
ACCESSION   DAOTFI010000408 DAOTFI010000000
VERSION     DAOTFI010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55509_length_2298_cov_6.194828"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1330)
                     /locus_tag="P5558_23825"
     CDS             complement(176..1330)
                     /locus_tag="P5558_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY27414.1"
                     /translation="MDGQPSGSGEIRLVDVTKTFGDMDAVKSIDLVIPHGSYCCLVGP
                     SGCGKTTILRMIAGHETPTSGEIHIGEQMVVGLKPVQRGTAMMFQSYALFPHLSVLDN
                     VAFYLKMRDVAKDERRRRAREMLERVQLDHLALRMPAMLSGGQQQRVALARALITNPR
                     VLLLDEPLSALDEFLRLRMRGELKSLQTELGITFVHVTHTQPEAIALADMVVVMDHGR
                     IDQADTARHIFSRPATPYVARFMGGQNVLSGTVTDTSGGVLVITDASGPTYAASASGP
                     MPGAGTAMSFVVRRDRIALTKGATAAPGERNAVNGRVIATEYQGTYVKVTLDVASREA
                     FVANIADDIYFAEPLKVGDEVTARWATADVHILERADTGAAIDPYAEDAA"
     gene            complement(1334..2164)
                     /locus_tag="P5558_23830"
     CDS             complement(1334..2164)
                     /locus_tag="P5558_23830"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27415.1"
                     /translation="MSLPSQGRRPWTFYVLSVMFTLFVLFLYGPMFIMFILSFQGPTG
                     GLVFPMRGFSLTWFDALFGQARTGDVGGAFSRSLPLALMVMVLTVLISVTAGFAFRRR
                     FVGSGFLFYTAVASLIVPGLVLSIGILVTARFLGIPARWWFTGLGAHLSWTLPFGLLI
                     MFAILGRLSPSYEEAAYDMGATRWQTVRHVVIPIVFPGMIAVALFGFTLSYDEFPRSW
                     LTVGSLNTLPIEIWTMTTNVTSPALYALGTVTSAISFVAIGLAIGSIAYIQHRRSKRT
                     "
BASE COUNT          408 a          712 c          854 g          324 t
ORIGIN      
        1 ctgatccgcc tctttcatcg gctcgtggcg ggcctgccgg cggacgggcc ggacgacgag
       61 gagcgggccg agctgcgccg cacgctctac ggcctgcacg ccatcctgcg cctgcatttc
      121 gcccaggagg acgagatcta cgagagcgtc gcggcggagg cgggccgctg agatttcagg
      181 cggcgtcctc ggcgtagggg tcgatggccg caccggtgtc ggcgcgctcg aggatgtgca
      241 cgtccgcggt cgcccagcgc gccgtcacct cgtcgccgac cttcaagggc tcggcgaaat
      301 agatgtcgtc ggcgatgttg gcgacgaagg cctcgcgcga cgccacgtcc agcgtcacct
      361 tgacgtaggt gccctggtac tcggtggcga tcacccgccc gttcaccgcg ttccgctcgc
      421 ccggcgccgc cgtcgcgcct ttcgtcaggg cgatccggtc gcggcgaacg acgaacgaca
      481 tggccgtgcc ggcgcccggc atgggtccgc tggcgctggc ggcgtaggtc ggcccgctgg
      541 cgtcggtgat caccagaacc ccgccggacg tgtccgtcac cgtgccggag agcacgttct
      601 ggccgcccat gaagcgggcc acatagggcg tggcgggccg cgagaagatg tggcgcgcgg
      661 tatcggcctg gtcgatccgc ccgtgatcca tgaccaccac catgtcggcg agggcgatgg
      721 cctcgggctg ggtgtgggtg acgtggacga aggtgatgcc gagctcggtc tgcaggctct
      781 tgagctcgcc gcgcatcctg aggcggagga actcgtcgag ggcggagagc ggctcgtcga
      841 gcagcagcac ccgggggttg gtgatcagcg cgcgcgccag cgccacccgc tgctgctggc
      901 cccccgagag catcgccggc atgcgcaggg ccagatggtc gagctgcacc cgctccagca
      961 tctcccgcgc ccgccggcgc cgctcgtctt tcgcgacatc gcgcatcttc agatagaagg
     1021 cgacgttgtc gagcaccgag agatgcggga agagcgcgta ggactggaac atcatggcgg
     1081 tgccgcgctg caccggcttg aggccgacga ccatctgctc gccgatgtgg atttcccccg
     1141 aggtcggcgt ctcgtgcccg gcgatcatcc tgaggatcgt cgtcttgccg cagcccgagg
     1201 ggccgacgag gcagcaatag gagccgtgcg ggatgacgag gtcgatcgac ttgaccgcat
     1261 ccatgtcgcc gaaggtcttg gtgacatcga cgaggcggat ctcgccgctg ccggagggct
     1321 gtccgtccat gcttcaggtt ctcttcgagc ggcggtgctg gatataggcg atcgagccga
     1381 tggcgaggcc gatcgcgacg aaggagatgg ccgaggtcac cgtgccgagg gcgtagaggg
     1441 ccggcgaggt gacgttggtg gtcatcgtcc agatctcgat cggcagcgtg ttcagggagc
     1501 ccacggtcag ccagctcctc gggaactcgt cataggagag ggtgaagccg aagagggcca
     1561 ccgcgatcat gccgggaaag acgatcggga tgacgacgtg gcggacggtc tgccagcggg
     1621 tcgcccccat gtcgtaggcc gcctcctcgt aggacgggct gagccggccg aggatggcga
     1681 acatgatcag aaggccgaag ggcagcgtcc aggagagatg ggcgccgagg cccgtgaacc
     1741 accagcgggc ggggatgccg aggaagcggg cggtgacgag gatgccgatc gagaggacga
     1801 ggcccggcac gatcaggctg gcgacggcgg tgtagaagag aaagcccgag ccgacgaacc
     1861 gccggcgaaa ggcgaagccg gccgtgaccg agatgagcac ggtcagcacc atgaccatga
     1921 gggcgagcgg cagcgagcgc gagaacgccc cgcccacgtc gccggtccga gcctggccga
     1981 agagcgcatc gaaccaggtg agcgagaagc cgcgcatggg aaagaccagc ccgcccgtcg
     2041 gcccctggaa cgacaggatg aacatgatga acatcgggcc gtagaggaag aggacgaaga
     2101 gcgtgaacat cacgctcagg acatagaagg tccaggggcg gcggccttgc gatggcaggg
     2161 acatcagcga acgagctcct tgcggacgtc gaccagccgg aagatcgcgg tggcgatcag
     2221 cagcacgatg atcagcagga cgacggcatt ggccgcggca cgcgggtagt tgaggaactg
     2281 caggtcgttg aagatgcc
//
LOCUS       DAOTFI010000409         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_56421_length_2270_cov_5.948533, whole genome shotgun sequence.
ACCESSION   DAOTFI010000409 DAOTFI010000000
VERSION     DAOTFI010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56421_length_2270_cov_5.948533"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="P5558_23835"
     CDS             complement(<1..637)
                     /locus_tag="P5558_23835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY27416.1"
                     /translation="MWPGDERPLRFGLVDVVVLAVLAGLLGWIVWLANTTLVYNWNWG
                     IVPSLFVRTDPETGGLAPNFLAEGLFTTLRLTFYAGIGAALIGLLIGLCRVANGLFFR
                     LVGRGYVEVVRNVPPLIFIFIFYFFVSSQIMPMIGIGNYARNLPVDERWWFELLFGDR
                     RRIDAFVAAVICLAMFEGAYVAEIIRSGIQSIPRGQWEAAASLGLSRFDRMR"
     gene            complement(700..1548)
                     /locus_tag="P5558_23840"
     CDS             complement(700..1548)
                     /locus_tag="P5558_23840"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY27417.1"
                     /translation="MKHLLNRLLDRRTALAAALVVGFAGGLAQAQDTRQAVAGDSVIE
                     QIKQRGSLIVGLSTFVPWAMLDKNGELIGYEIDVATKLAEDMGVEVEFVPTAWDGIIP
                     ALISGTFDVIISGMSVTPQRNLTVNFSEPYSFSGLRVVVSREHEGAISGLEDMNRADF
                     TIAVRRGATPVTFAQQALPNARLLQFDEDGAAIQEVLNGNADAVIASEPSPSAYAAEY
                     PDKVFIPFDDLFSVTAEAMALRKGDPDALNFFNNWIAVNWRNGFLEERHDYWFRTQGW
                     ADQLPQ"
     gene            complement(1604..>2270)
                     /locus_tag="P5558_23845"
     CDS             complement(1604..>2270)
                     /locus_tag="P5558_23845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY27418.1"
                     /translation="RVPDYIVRVVDERLVWGPLAFGFVETVRLSAISFVSTVLLGLAV
                     AVLRLARSQVGRAVAIGYTELIRNTPLLVQLYIIYFVLAPIVGMDRNWTAIFCLTLFE
                     AAFAAEIFRAGIMSVPKGQWEAAASLGLEKRQAYRDIILPQAVRFVLPPLTGLAVSLV
                     KHSSIVSVIAVFELTTEGRNIISDTYMSFEIWLTVAGLYLSITVTLSLLAAWLERRFE
                     VAS"
BASE COUNT          427 a          758 c          764 g          321 t
ORIGIN      
        1 cgcgcatccg gtcgaagcgc gacaggccga ggctcgccgc cgcctcccac tggcccctgg
       61 ggatcgactg gatgccgctt cggatgatct cggccacata ggcgccctcg aacatggcga
      121 ggcagatgac ggcggcgacg aaggcgtcga tccgccgccg gtcgccgaac agcagctcga
      181 accaccagcg ctcgtccacc ggcaggttgc gggcgtagtt gccaatgccg atcatgggca
      241 tgatctgcga gctgacgaag aagtagaaga tgaagatgaa gatcaaaggc gggacgttgc
      301 ggaccacctc gacatagccc cgcccgacca gccggaagaa gagcccgttg gccacccggc
      361 agagaccgat caaaaggccg atcagcgcag ccccgatgcc ggcatagaag gtgagccgga
      421 gcgtggtgaa caggccctcg gccaggaagt tcggcgcgag cccgccggtc tcgggatcgg
      481 tccgcacgaa caggctcggc acgatgcccc agttccagtt gtagacgagg gtcgtgttgg
      541 cgagccagac gatccagccg agcagcccgg ccaggaccgc cagcaccacg acgtcgacca
      601 gcccgaagcg cagcggccgc tcgtcacccg gccacatgaa gcctcaaccg atggaaagcc
      661 ggccggcggc cgcccgcgcg ggcgaccgcc gcacccggcc tactgcggca gttgatcggc
      721 ccagccctgc gtgcggaacc agtaatcgtg gcgctcctcg agaaagccgt tgcgccagtt
      781 cacggcgatc cagttgttga agaaattcag ggcatccggg tcacccttgc gcagcgccat
      841 ggcctcagcc gtcacgctga agagatcgtc gaacgggatg aagaccttgt ccggatactc
      901 ggcggcgtag gcgctgggcg agggctccga cgcaatgacc gcgtcggcat tgccgttgag
      961 cacctcctgg atcgcggcac catcctcgtc gaactgcagg agccgcgcat tgggcagggc
     1021 ctgctgcgcg aacgtcaccg gcgtggcgcc ccggcgcacg gcaatcgtga agtccgcccg
     1081 gttcatgtcc tcgaggcccg agatcgcgcc ctcgtgctcg cgcgacacca cgaccctgag
     1141 gcccgagaag gaatagggct cggagaagtt gaccgtcagg ttgcgctgcg gggtgacgct
     1201 catgccggag atgatgacgt cgaacgtgcc ggagatcagg gccgggatga tgccgtccca
     1261 ggcggtcggc acgaactcga cctccacgcc catgtcctcg gccagcttcg tggcgacgtc
     1321 gatctcgtag ccgatcagct cgccgttctt gtcgagcatg gcccagggca cgaaggtcga
     1381 gagcccgacg atcaggctgc cgcgctgctt gatctgctcg atcacgctgt cgccggccac
     1441 cgcctggcgc gtgtcctgcg cctgcgccag cccgccggcg aaaccgacga ccagggccgc
     1501 cgccagggcg gtccttcgat ccaataggcg gttcagtaga tgcttcacgg ctttcctccg
     1561 cttttcttca tcagtgatgc gtgcccgtgc tgccgatccg gcgtcacgag gcgacctcga
     1621 accgccgctc gagccaggcc gcgagcagcg agagcgtgac cgtgatggag agatagagcc
     1681 cggccacggt cagccagatc tcgaagctca tatacgtgtc ggagatgatg ttgcgacctt
     1741 ccgtcgtcag ctcgaagacg gcgatcacgc tgacgatcga gctgtgcttg acgagcgaga
     1801 ccgcgagccc ggtcaggggc ggcaggacga agcgcacggc ctgcggcagg atgatgtcgc
     1861 gataggcctg ccgcttctcg agcccgaggc tcgccgccgc ctcccactgg cccttcggca
     1921 ccgacatgat gccggccctg aagatctcgg ccgcgaacgc cgcctcgaac agcgtcaggc
     1981 agaagatcgc ggtccagttg cggtccatgc cgacgatcgg cgccagcacg aagtagatga
     2041 tgtagagctg gaccagaaga ggcgtgttgc ggatcagctc ggtgtagccg atcgccaccg
     2101 cgcggccgac ctgcgagcgg gcgaggcgga gcacggcgac cgcgagcccg agcagcacgg
     2161 tcgagacgaa ggagatcgcc gagaggcgaa cggtctcgac gaagccgaag gccaggggtc
     2221 cccagaccag ccgctcgtcg acgacgcgca cgatgtaatc cggcacgcgg
//
LOCUS       DAOTFI010000410         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_57637_length_2235_cov_5.680734, whole genome shotgun sequence.
ACCESSION   DAOTFI010000410 DAOTFI010000000
VERSION     DAOTFI010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57637_length_2235_cov_5.680734"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..719
                     /locus_tag="P5558_23850"
     CDS             297..719
                     /locus_tag="P5558_23850"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY27419.1"
                     /translation="MRIRTTRTTRFAAALAALSLVVAACGGDDDEGADTATAGQGADT
                     ATAEPTATAEEPAPAGGEGGETIRLWLNGGDTPDELVEYAVTEFNKIHPDVEVQFERQ
                     QWTGIVEKLTTSLSSTVKVKSISASSETFFSRLPTRLT"
     gene            complement(919..1662)
                     /locus_tag="P5558_23855"
     CDS             complement(919..1662)
                     /locus_tag="P5558_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27420.1"
                     /translation="MLALAGGVAAAPALPGGALAAEILLEAGGAAAIGRLRPAVELRL
                     EGGGTAVLRDIFVPEAVDAGARGLLAPLLARAALDLAGPAGTDRYGRLQARPLAADGR
                     SLQAELVAAGLALVRPLPDSDPVVLETLLREEALAEAAGLGLWRRPADVLVDEAAAGS
                     MLGQFVLVEGVVLQASRQQRYFYLNFGTDWRTDTTARIDKETLRAMQRAGFDPAALEG
                     RNVRLRGTLFAENGPMLELWTHRGIEVLP"
     gene            1722..1798
                     /locus_tag="P5558_23860"
     tRNA            1722..1798
                     /locus_tag="P5558_23860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1756..1758,aa:Arg,seq:cct)
     gene            2005..>2235
                     /locus_tag="P5558_23865"
     CDS             2005..>2235
                     /locus_tag="P5558_23865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27421.1"
                     /translation="MTSISYQLYSSRKDPDPAGVLPALAALGYERVEGYGGLFEDQAR
                     LARLKADLASTGLAMPTAHFGIEMVEKEPDRVV"
BASE COUNT          374 a          836 c          758 g          267 t
ORIGIN      
        1 accacgttcc gcgaggcgat cagcgatgcc gtcgccgccc ggaccatgcg cgagatcagc
       61 gatgccctga ccgtgcaccc gtcgagtgtt ggcgacgacg acgtcatcct cggcgcagcg
      121 gcgctcgtcc tcgaccacga gctggggatc cgatgagacg cgccggcctc tccgccccat
      181 cgccccggac ctgaccgaac gatctcgccg accctggagg ggagccaggg tcggcccgcc
      241 cggctcgccg ggcggcgcca gaagcgcccc gtcacccatc ccgaggagga aacaccgtga
      301 ggatccgaac caccagaacc actcgcttcg ctgcggcgct cgccgcgctc agcctggttg
      361 tcgccgcatg cggtggcgac gacgacgagg gcgccgacac cgccaccgcc ggacagggcg
      421 ccgacaccgc caccgccgag ccgacagcga cggccgagga gccggcaccg gccggcggcg
      481 agggcggcga gacgatccgc ctctggctga acggtggcga cacacccgac gagctcgtcg
      541 agtacgcggt caccgagttc aacaagatcc accccgacgt cgaggtgcag ttcgagcgcc
      601 agcagtggac cggcatcgtc gagaagctga ccacctcgct gtcgagcacg gtgaaggtga
      661 agtcgatctc ggcgagctcc gagacgttct tcagccggtt gccgacccgg ctcacatagg
      721 cggcgagctc ggggtcgtcg acggccccac cgtactctgc gaggatctgc gggtgggcct
      781 cgcgcccggc gcggacgtcg tcctcgaccg agcccgtggc gaggatcggc ccgccgccgg
      841 ccggtgcggt cgtgcaggcg gaggtcaggg ccagcgaaac cgacagcgtc gcggcgagaa
      901 gggcggtgcg cgtacgaatc acggcaagac ctcgattccc cgatgtgtcc aaagctcgag
      961 catcggcccg ttctcggcga acaacgtgcc acgcaggcgc acgttccggc cctcgagcgc
     1021 cgccggatcg aagcccgccc gctgcatggc gcgcaaggtc tccttgtcga tgcgtgccgt
     1081 ggtgtcggtg cgccagtcgg ttccgaagtt gaggtagaaa tagcgctgct gccggctcgc
     1141 ctgcaagacc accccctcga cgagcacgaa ctggccgagc atcgagccgg ccgccgcctc
     1201 gtcgaccagc acgtccgccg ggcgccgcca cagcccgagc ccggccgcct cggccagcgc
     1261 ctcctcccgc agcagcgtct cgagcaccac gggatcgctg tccggcaggg gccggacgag
     1321 ggcgaggccc gcggccacca gctcggcctg caacgaccgc ccgtcggcag cgagcggccg
     1381 ggcctgcagc cggccgtagc gatcggtgcc ggccggcccg gcgaggtcga gcgccgcccg
     1441 ggccagcagc ggcgccagca gccccctcgc cccggcgtcg accgcctcgg gcacgaagat
     1501 gtcgcgcagc accgccgtgc cgccaccctc gagccgcagc tcgacggccg gccgcagccg
     1561 gccgatggcc gcggccccgc ccgcctccag caggatctcc gccgccaggg caccgccggg
     1621 cagcgccggc gccgcagcca cgccgccggc aagcgccagc acgagaccca gccagcggag
     1681 ttgtcttgac caccatgggc ttgatatacc cgacggcggc ggcgcccgta gctcagcagg
     1741 atagagcatc agattcctaa tctgagggtc acaggttcga gtcctgtcgg gcgcgccagc
     1801 atcgacagaa gacaagaaac aggcgatcgg cgctccctgg agcgttcgac gttgggttcg
     1861 cgccatccac gagcaaccct ccgatcaggc acgtgtcgag gcgaatgtaa ggctcgccgc
     1921 aagaacccgc gaggtctttc acaaccccgg gaacatcgcc gaacgcaacc cacgcagaag
     1981 cggacagtct cccgaaagga ccagatgacc agcatctcct atcagctcta ctcgtcccgc
     2041 aaggacccgg atccggccgg ggtgctgccg gcgctggcgg cgctcggcta cgagcgggtc
     2101 gagggctatg gcggcctttt cgaggaccag gcccggctcg cccggctgaa ggccgacctg
     2161 gccagcaccg ggctcgccat gccgaccgcc catttcggca tcgagatggt ggagaaggag
     2221 cccgaccggg tcgtc
//
LOCUS       DAOTFI010000411         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_57892_length_2228_cov_7.416475, whole genome shotgun sequence.
ACCESSION   DAOTFI010000411 DAOTFI010000000
VERSION     DAOTFI010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57892_length_2228_cov_7.416475"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="P5558_23870"
     CDS             <1..371
                     /locus_tag="P5558_23870"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRY27422.1"
                     /translation="SICIRWQGLRLVGRARAQLQAGEAPVFEVVSGVCLVVAGALLLT
                     PGFVTDTAGFLLLLPPVRRLLFDRVLAARLRGMARAPGGAEDAGARRRPRPVVIEGEL
                     EPLDEAGEMPPPRGGWDRPR"
     gene            368..928
                     /locus_tag="P5558_23875"
     CDS             368..928
                     /locus_tag="P5558_23875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY27423.1"
                     /translation="MILLRHGEGTWNRHFHAVRVDAGIHDPALTETGHAQARAAAERL
                     AGTGARRLIASPYRRTLETASRIADRLGLDIEVEPLVRERCAFSCDQGSPASVLARDW
                     PMLDFGALDEIWWGRRIESQASLEARAGVFLRRMAEASDLAATIVVTHWGFIRAVTGQ
                     AIGNAEQVRFMPNGPDAETRGQTARH"
     gene            940..1476
                     /gene="secB"
                     /locus_tag="P5558_23880"
     CDS             940..1476
                     /gene="secB"
                     /locus_tag="P5558_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRY27424.1"
                     /translation="MSDETTGPNGGTSPAGAGAAAAEQAPPRLAILTQYIKDMSFENP
                     RAPQPPQQAGSRPEIQIKVDVQAKAMGEERYEVTLVTNVDAKSSGETIFLMELTYAGV
                     FHLANIPQDSLQPLVMIECPRLLFPFARRIVADATRDGGFPPLMIDPIDFVALYRRKL
                     QEAQAKAQAGAVPPPVAS"
     gene            complement(1507..>2228)
                     /locus_tag="P5558_23885"
     CDS             complement(1507..>2228)
                     /locus_tag="P5558_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRY27425.1"
                     /translation="HSQRLIDSGKILGFDVPYSVAELDAATMAVIRKNGFSDCYVRPL
                     AWRGSEQMGVSAQHSRINVAIATWEWGAYYSDLTLTRALYQRPAPNTAPVKSKAAGLY
                     MICTICKHDAERKGFGDALMLDYRGYLAETTGANLFLVIDGVLHTPAPDCFLDGITRR
                     TVIELARQRGYEVVERHIRPEELENVQEIFVTGTAAEVTPVRAIDEMKFQVGPITSSL
                     VEDFRALTRAQPVAAATARAS"
BASE COUNT          311 a          772 c          812 g          333 t
ORIGIN      
        1 gcagcatctg catccgctgg cagggcctgc ggctggtcgg gcgggcccgg gcccagctcc
       61 aggcgggcga ggcacccgtc ttcgaggtcg tgtcgggggt ctgcctcgtg gtcgccggcg
      121 ctctcctgct gacgccgggc ttcgtcaccg acacggccgg ctttctgctg ctgctgccgc
      181 cggtccgtcg cctgctgttc gaccgggtgc tggcggcccg gctgcgcggc atggcccggg
      241 cgccgggggg cgccgaggat gccggcgcgc gacgccggcc ccggcccgtg gtcatcgagg
      301 gcgagctcga gccgctcgac gaggcgggcg agatgccgcc gccgcggggc ggctgggacc
      361 ggccccgatg atcctcctgc ggcacggcga gggcacctgg aaccggcatt tccatgccgt
      421 gcgggtcgat gccgggatcc acgacccggc gctgaccgag accggccacg cgcaggcccg
      481 ggcggcggcc gagcggctcg ccggcacggg cgcccgccgc ctgatcgcca gcccctaccg
      541 gcgcacgctc gagacggcga gccgcatcgc cgaccgtctc gggctcgaca tcgaggtcga
      601 accgttggta cgagagcgtt gcgcgttctc ctgcgatcag ggcagccccg cctcggtcct
      661 cgcccgcgac tggccgatgc tcgatttcgg cgctctcgac gagatctggt ggggccggcg
      721 gatcgagagc caggcctcgc tcgaggcccg ggcgggcgtt tttctgcggc gcatggccga
      781 agcaagcgat ctcgcggcga cgatcgtcgt gacgcattgg gggttcatcc gggccgtgac
      841 cgggcaggcg atcggcaacg ccgagcaggt ccgcttcatg ccgaacgggc cagacgccga
      901 aactcgcggc caaacagcca gacattgagg aatgagacga tgagtgacga gaccacgggc
      961 cccaacggcg gcacctcccc ggccggggcc ggcgctgccg cagccgagca ggcgccgccg
     1021 cgcctcgcga tcctgaccca gtacatcaag gacatgtcgt tcgagaaccc gcgggcaccc
     1081 cagccgccgc agcaggcggg gagccggccg gagatccaga tcaaggtgga tgtccaggcc
     1141 aaggcgatgg gcgaggagcg ctacgaggtg acgctggtca ccaatgtcga cgccaagtcg
     1201 agcggcgaga cgatcttcct catggagctg acctatgcgg gcgtcttcca cctcgccaac
     1261 atcccgcagg acagcctgca gccgctcgtg atgatcgagt gcccgcggct gctcttcccg
     1321 ttcgcccggc ggatcgtcgc cgacgcgacg cgggatggcg gctttccgcc tttgatgatc
     1381 gatccgatcg acttcgtcgc cctctaccgg cgcaagctgc aggaggccca ggcgaaggcg
     1441 caggccgggg cggtgccgcc gcccgtcgcg agctgaggtc gggcccccgg ttcagcccgg
     1501 gcgggctcag ctcgcccggg cggttgccgc ggccactggc tgggcccggg tcagggcgcg
     1561 gaagtcctcg accagcgagc tggtgatcgg gcccacctgg aacttcatct cgtcgatggc
     1621 cctgaccggg gtcacctcgg ccgccgtgcc ggtgacgaag atctcctgca cgttctccag
     1681 ctcctccggc ctgatgtggc gctcgaccac ctcgtagccg cgctgcctcg ccagctcgat
     1741 caccgtccgc cgggtgatgc cgtcgaggaa gcaatccggt gccggggtat ggaggacacc
     1801 gtcgatgacg aggaagaggt tcgcgccggt ggtctcggcg agatagcccc ggtagtcgag
     1861 catcagcgcg tcgccgaagc ccttgcgctc ggcgtcgtgc ttgcagatcg tgcagatcat
     1921 gtagagcccg gcggccttgc tcttgaccgg ggccgtgttg ggggcaggcc gctggtagag
     1981 ggcgcgggtc agggtgaggt cgctgtagta ggcgccccat tcccaggtgg cgatggcgac
     2041 attgatgcgg ctgtgctggg cggagacccc catctgctcc gagccgcgcc aggccagcgg
     2101 ccggacatag cagtcggaga agccgttctt gcggatcacc gccatggtcg cggcatccag
     2161 ctcggctacc gagtagggca cgtcgaagcc caggatcttg ccgctgtcga tcaaccgctg
     2221 gctgtgct
//
LOCUS       DAOTFI010000412         2218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_58251_length_2218_cov_7.178456, whole genome shotgun sequence.
ACCESSION   DAOTFI010000412 DAOTFI010000000
VERSION     DAOTFI010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58251_length_2218_cov_7.178456"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1292)
                     /locus_tag="P5558_23890"
     CDS             complement(48..1292)
                     /locus_tag="P5558_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRY27426.1"
                     /translation="MTEKPTNIRIDGDRLMQTIFEMAKIGATPKGGCNRQTLTDLDRE
                     GRDLFRSWCEAAGCTVEIDELGNMFARRPGKRPELPPVVMGSHLDTQPTGGKYDGIAG
                     VLTGLEVIRTLNDFNFETERPIEVVNWTNEEGTRFAPAMLASGVYGGAFTRDWAYALQ
                     GFDGATFGAELERIGYKGERPCAPQEWDSFFELHIEQGPILEAEGKTIGVVTGGQGMR
                     WYNVTITGRESHAGTTPIDRRKDALLAASKVFVRLREIAYEHGPYGVTTVGHVDVQPN
                     SRNVIPGEVMFTADVRHPEADHLEAMGEAFRAAVAEACALEGLDSTIDEIWYFPPVAF
                     DADRVAAVRRAAEAAGLSHRDMVSGAGHDACYVARVAPTAMIFIPCKDGLSHNEAEHA
                     EPEHLIAGCDVLLRAVLERASG"
     gene            complement(1326..>2218)
                     /gene="preA"
                     /locus_tag="P5558_23895"
     CDS             complement(1326..>2218)
                     /gene="preA"
                     /locus_tag="P5558_23895"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HRY27427.1"
                     /translation="GVELNFGCPHGMSERGMGSAVGQVPEYIEMVTRWCKQHTRMPVF
                     VKLTPNVTNILAPARAAVAGGADAVSLINTINSITAVDLDRMAPEPVVNGKGAHGGYC
                     GPAVKPIALCMVAEIARDPDCAGVPISGIGGIETWRDAAEFMALGAGSVQVCTAAMHY
                     GFKIVEDMNDGLANWMDDKGYATLDGFRGAAVGNYTDWGKLDMNYDIKARISDELCIG
                     CGLCHIACEDTSHQAIRFTQDAGKRRFTVIDADCVGCNLCMHVCPVEGCITMEEVSEG
                     RPYLTWAAHPKNPQNLKAAE"
BASE COUNT          326 a          747 c          767 g          378 t
ORIGIN      
        1 aagtccaagc cgaaatacag catggccctg cattccttca cacgctctca gccgctggcg
       61 cgctcgagca cggcgcggag cagcacgtcg cagccggcga tcaggtgctc gggctcggca
      121 tgctccgcct cgttgtggct gagcccgtcc ttgcagggga tgaagatcat cgccgtcggc
      181 gccacccggg cgacatagca ggcgtcgtgg ccggcgccgg agaccatgtc gcggtggctg
      241 agcccggccg cctccgcggc gcggcgcacg gcggccaccc ggtcggcgtc gaaggcgacg
      301 ggcgggaagt accagatctc gtcgatggtg gaatccagcc cctcgagcgc gcaggcctcg
      361 gcgaccgcgg cgcggaaggc ctcgcccatc gcctcgagat ggtcggcctc gggatggcgg
      421 acgtcggcgg tgaacatcac ctcgccgggg atcacgttgc gcgagttggg ctgcacgtcc
      481 acatggccga ccgtggtgac cccgtagggc ccgtgctcgt aggcaatctc gcgcagtcgg
      541 acgaagacct tcgacgcggc gaggagcgca tccttgcggc gatcgatcgg cgtggtgccg
      601 gcatggctct cgcggccggt gatggtcacg ttgtaccagc gcatgccctg cccgccggtg
      661 accacgccga tggtcttgcc ctcggcctcg agaatcggcc cttgctcgat atgcagctcg
      721 aagaagctgt cccattcctg cggcgcgcag ggacgctcgc ccttgtagcc gatccgctcg
      781 agctcggcgc cgaaggtggc gccatcgaaa ccctggagcg cgtaggccca gtccctggtg
      841 aaggcgccgc catagacacc ggaggcgagc atggccgggg cgaaacgcgt gccctcctcg
      901 ttggtccagt tgaccacctc gatcggccgc tcggtctcga agttgaaatc gttcagcgtg
      961 cggatcacct cgagcccggt caggacgccg gcgatgccgt catacttgcc accggtcggc
     1021 tgggtgtcga gatggctgcc catgacgacc ggcggcagct ccgggcgctt gcccgggcgg
     1081 cgggcgaaca tgttgccgag ctcgtcgatc tcgaccgtgc agccggccgc ctcgcaccac
     1141 gagcggaaga ggtcgcggcc ctcgcggtcg aggtcggtca gcgtctgccg attgcagccg
     1201 cccttgggcg tggcgccgat cttcgccatc tcgaagatgg tctgcatcag ccgatcgccg
     1261 tcgatcctga tgttggtcgg cttctcggtc accggtcgat ctcccaaatg cgtcttcgct
     1321 ggcggctact cggccgcctt caggttctgc gggttcttcg gatgcgccgc ccaggtcaaa
     1381 tagggccgcc cctcgctcac ctcctccatg gtgatgcagc cctcgaccgg gcagacatgc
     1441 atgcagagat tgcagccgac gcagtcggcg tcgatcacgg tgaagcggcg cttgcccgcg
     1501 tcctgggtga agcggatggc ctggtgcgag gtgtcctcgc aggcgatgtg gcagagaccg
     1561 cagccgatgc agagctcgtc gctgatccgc gccttgatgt cgtagttcat gtcgagcttg
     1621 ccccagtcgg tatagttgcc gaccgcggcg ccgcggaaac cgtcgagcgt ggcgtagccc
     1681 ttatcgtcca tccagttggc gagaccgtcg ttcatgtcct cgacgatctt gaagccgtaa
     1741 tgcatggcag cggtgcagac ctgcaccgag ccggcgccga gcgccatgaa ctcggcggcg
     1801 tcgcgccagg tctcgatgcc gccgatgccg gagatcggca cgccggcgca gtccgggtcg
     1861 cgggcgatct cggccaccat gcagagggcg atgggcttga ccgccgggcc gcaatagccg
     1921 ccatgcgccc ccttgccgtt gaccacgggc tcgggcgcca tgcggtcgag gtcgacggcg
     1981 gtgatcgagt tgatggtgtt gatcagcgag accgcgtcgg cgccgccggc gaccgccgcc
     2041 cgggcggggg ccaggatgtt ggtgacgttt ggcgtgagct tgacgaagac cggcatcctc
     2101 gtgtgctgct tgcaccagcg ggtcaccatc tcgatgtact cgggcacctg gcccacggcc
     2161 gagcccatgc cgcgctcgct catgccgtgc gggcagccga aattgagttc gaccccgt
//
LOCUS       DAOTFI010000413         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_59119_length_2194_cov_7.232819, whole genome shotgun sequence.
ACCESSION   DAOTFI010000413 DAOTFI010000000
VERSION     DAOTFI010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59119_length_2194_cov_7.232819"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="P5558_23900"
     CDS             complement(<1..148)
                     /locus_tag="P5558_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27428.1"
                     /translation="MNEAVATENVGKDNIATGRITRVTGAVVDVQFDGPLPAILNALH
                     TENAG"
     gene            complement(186..1091)
                     /locus_tag="P5558_23905"
     CDS             complement(186..1091)
                     /locus_tag="P5558_23905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRY27429.1"
                     /translation="MPSLKELRGRIGSVKQTQKITSAMKLVAASKLKRAQEQAEASRP
                     FALKMSAMLANLAAGVRDLPGAPPLLAGSGKEETHLIVVATSDRGLCGGFNSSIVRAV
                     RRRIAELLDAGKAVKIICIGRKGRDMLRREHGKLIIEFVDGVAGKRRMEYADAALVAQ
                     KILDLYAAGEFDVCSIIFNRFKSAVTQIVTLQQIVPVEPPAPVEDTAAASQAEGGAIY
                     EFEPDEEQILSELLPRNLAVQIFGALVENAASEQGARMAAMDNATRNAGEMIRKLTLR
                     YNRQRQAQITKELIEIISGAEAAVG"
     gene            complement(1099..>2194)
                     /gene="atpA"
                     /locus_tag="P5558_23910"
     CDS             complement(1099..>2194)
                     /gene="atpA"
                     /locus_tag="P5558_23910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRY27430.1"
                     /translation="TGLKSVDSMVPIGRGQRELIIGDRQTGKTAIAIDCFINQKPYNE
                     GAGDDEHKKLYCIYVAIGQKRSSVAKLVKTLQETGALEYTIIVAATASDPAPLQYLAP
                     YTACTMGEYFRDNGMHAVIVYDDLSKQATAYRQMSLLLRRPPGREAFPGDVFYLHSRL
                     LERAAKLNDENGKGSLTALPVVETQANDVSAYIPTNVISITDGQIFLETDLFFQGIRP
                     AVNVGLSVSRVGSAAQTKAMKKVAGRIKLELAQYREMAAFAQFASDLDVATQRLLARG
                     ARLTELLKQPQYQPMPMEEQACVIFAGVNGYLDGVSVANIGRYEQGLLSELRSSGSSI
                     LASVRDTGDLKPEVEKELRALIEAYTKSFG"
BASE COUNT          306 a          700 c          784 g          404 t
ORIGIN      
        1 tgccggcgtt ctcggtgtgg agcgcgttca ggatcgccgg cagcggtccg tcgaactgca
       61 cgtcgacgac ggctccggtg acgcgggtga tccgccccgt ggcgatgttg tccttgccga
      121 cgttctctgt ggctacggcc tcgttcatgc ttgggctccc agcgtccagg ttcgggtgtg
      181 agcggtcatc cgacggccgc ctcggcaccc gagatgatct cgatcagttc cttggtgatc
      241 tgggcctgcc gctggcggtt gtagcgcagc gtcagcttcc tgatcatctc gcccgcattg
      301 cgggtggcgt tgtccatggc ggccatcctg gccccctgct cgctggccgc gttctcgacc
      361 agggcgccga agatctgcac ggcgaggttg cgcggcagta gctcggagag gatctgctcc
      421 tcgtccggct cgaactcgta gatggcaccg ccctcggcct ggctggccgc cgcggtgtcc
      481 tcgaccggcg ccggcggctc gaccggcacg atctgctgca gggtcacgat ctgggtgact
      541 gccgacttga agcggttgaa gatgatggag cagacgtcga actcgccggc ggcgtagagg
      601 tcgagaatct tctgcgccac cagtgccgcg tcggcatatt ccatgcggcg cttgccggcg
      661 acgccgtcga cgaactcgat gatcagcttg ccgtgctcgc gacgcagcat gtcgcggccc
      721 ttgcggccga tgcagatgat cttgaccgcc ttgccggcat ccaggagctc ggcgatgcgg
      781 cggcggaccg cccggacgat cgagctgttg aagccgccgc agaggccacg atcggaggtg
      841 gcgacgacga tgaggtgcgt ctcctccttg ccgctgccgg caagcagcgg gggtgcgccc
      901 ggcaggtcgc gcacgccggc cgcgagattg gccagcatcg ccgacatctt cagcgcgaac
      961 ggccgcgacg cctcggcctg ctcctgggca cgcttcagct tggaagcggc cacgagcttc
     1021 atcgcactcg tgatcttctg cgtctgcttg acgctgccga tccggccgcg gagctccttg
     1081 aggctcggca tcggcggctc agccgaagct cttggtgtag gcctcgatca gcgcgcgcag
     1141 ctccttctcg acctcgggct tgaggtcgcc ggtgtcgcgc accgaggcca ggatcgagct
     1201 gccgctgctg cgcagctcgc tgagcaggcc ctgctcgtag cggccgatat tggcgaccga
     1261 cacgccgtcg aggtagccgt tgacgcccgc gaagatgacg caggcctgct cctccatcgg
     1321 catcggctgg tactgcggct gcttcaagag ctcggtcagc cgggcgccgc gggccaagag
     1381 gcgctgggtg gcgacgtcga ggtccgaggc gaactgggcg aaggcggcca tctcgcggta
     1441 ctgggcgagc tcgagcttga ttcgccctgc gaccttcttc atcgccttgg tctgggccgc
     1501 cgagccgacg cggctgaccg agaggccgac attcaccgcc gggcggatgc cctggaagaa
     1561 gaggtcggtc tcgaggaaga tctggccgtc ggtgatcgag atgacgttgg tcgggatgta
     1621 ggccgagacg tcgttggcct gggtctcgac caccggcagc gccgtgagcg agcccttgcc
     1681 gttctcgtcg ttgagcttgg ccgcccgctc gagcaggcgc gaatggaggt agaagacgtc
     1741 gcccgggaag gcctcgcggc cgggcgggcg gcgcagcagg agcgacatct ggcgataggc
     1801 cgtcgcctgc ttggagaggt cgtcgtagac gatcacggcg tgcatgccgt tgtcacgaaa
     1861 gtactcgccc atggtgcagg cggtgtaggg ggcgaggtac tgcagcggcg ccgggtcgga
     1921 ggcggtggcg gcgacaatga tcgtgtactc gagcgcgccg gtctcctgca gcgtcttgac
     1981 cagcttggcc accgacgagc gcttctggcc gatggcgacg tagatgcagt agagcttctt
     2041 gtgctcgtcg tcgccggccc cttcgttgta gggcttctgg ttgatgaagc agtcgatggc
     2101 gatggcggtc ttgccggtct ggcggtcgcc gatgatcagc tcgcgctggc cacggccgat
     2161 cggcaccatc gagtcgaccg acttcaggcc ggtc
//
LOCUS       DAOTFI010000414         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_61784_length_2124_cov_7.948284, whole genome shotgun sequence.
ACCESSION   DAOTFI010000414 DAOTFI010000000
VERSION     DAOTFI010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61784_length_2124_cov_7.948284"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /gene="secA"
                     /locus_tag="P5558_23915"
     CDS             complement(<1..2004)
                     /gene="secA"
                     /locus_tag="P5558_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRY27431.1"
                     /translation="MLGAIARKLFGTTNDRLVKSARKTVDEINGLEPAFEALDDAAIR
                     AKTEEFRSRLAAGETLDALLPEAFATVREAAKRTLGQRHFDVQLLGGMFLHQGAIAEM
                     KTGEGKTLVSTLAAYLNALPGKGVHLVTVNDYLARRDSRWMGQIYEFLGLTVGCIVPG
                     IGEADRRAAYAADITYGTNNEFGFDYLRDNMKLSLETMVQRAHNFAIVDEVDSILVDE
                     ARTPLIISGPAEGGSELYVAINQLIPQLGPDDFEQDEKQRTVTLTEAGTERMHQVLTE
                     AGLLKDGDLYDPQNITLVHHVQEALRAHKIFNRDVDYLVKDDKVIIVDEFTGRMMEGR
                     RYSGGLHQALEAKEGVSIQPENQTLASITFQNYFRLYEKLAGMTGTAATEAAEFAEIY
                     KLEVIEIPTHRSMIRVDEDDEVYRTGREKNEAIVKEILASHAKGQPVLVGTVSIEKSE
                     ALATLLKKEKIPFHVLNARYHEQEAFIIAQAGRVGGVTIATNMAGRGTDIQLGGNAEM
                     RIQQELGAEPDPERARAIEAEVRAEGEKVKAAGGLYVIGTERHESRRIDNQLRGRSGR
                     QGDPGRSKFFLSLDDDLMRIFGSERMDGMLQKLGLKEGEAIIHPWINKALEKAQQKVE
                     ARNFEIRKNLLKFDDVMNDQRKVVYEQRKELMSAEDVSHTVTDM"
BASE COUNT          295 a          684 c          726 g          419 t
ORIGIN      
        1 catgtcggtg acggtgtgcg agacgtcttc cgcgctcatc agctccttgc gctgctcgta
       61 gacgaccttg cgctggtcgt tcatgacgtc gtcgaacttc agcaggttct tgcggatctc
      121 gaagttgcgc gcctcgacct tctgctgcgc cttctcgagc gccttgttga tccagggatg
      181 gatgatcgcc tcgccctcct tgaggccgag cttctgcagc atcccgtcca tgcgctccga
      241 gccgaagatg cgcatcaggt cgtcgtcgag cgagaggaag aacttggacc ggccgggatc
      301 gccctggcgg cccgaccggc cgcggagctg gttgtcgatg cgccggctct cgtgccgctc
      361 ggtgccgatc acgtagaggc cgccggccgc cttgaccttc tcgccctcgg ccctgacctc
      421 ggcctcgatc gccctggcgc gctcgggatc gggctcggcg ccgagctcct gctggatccg
      481 catctcggca ttgccgccga gctggatgtc ggtgccgcgc ccggccatgt tggtggcgat
      541 ggtcaccccg ccgacccggc cggcctgggc gatgatgaag gcttcctgct cgtgatagcg
      601 tgcgttgagc acgtggaagg ggatcttctc cttcttgagc agggtcgcca gcgcctccga
      661 cttctcgatc gagacggtgc cgaccagcac cggctggccc ttggcatgcg acgccaggat
      721 ctccttgacg atcgcctcgt tcttctcgcg gccggtgcgg tagacctcgt cgtcctcgtc
      781 gacccggatc atcgaccggt gggtcgggat ctcgatcacc tcgagcttgt agatctcggc
      841 gaactcggcc gcctcggtcg ccgcggtgcc ggtcatgccg gcgagcttct cgtagaggcg
      901 gaagtagttc tggaaggtga tcgacgccag cgtctggttc tccggctgga tcgagacgcc
      961 ttccttggct tccagcgcct ggtgcagccc gccagagtag cgcctgcctt ccatcatgcg
     1021 gccggtgaac tcgtcgacga tgatcacctt gtcgtccttg accaggtagt cgacgtcgcg
     1081 attgaagatc ttgtgcgccc gcagcgcctc ctgcacgtga tggacgagcg tgatgttctg
     1141 cgggtcgtag agatcgccgt ccttgagcag gccggcctcg gtcagcacct ggtgcatgcg
     1201 ctcggtgccg gcctcggtca gggtcaccgt gcgctgcttc tcgtcctgct cgaaatcatc
     1261 ggggccgagc tgtggaatga gctggttgat ggcgacgtag agctcggagc cgccttcggc
     1321 cgggccggag atgatgagcg gggtgcgcgc ctcgtcgacc aggatcgaat cgacctcgtc
     1381 gacgatggcg aagttgtgcg cccgctgcac catggtctcg agcgagagct tcatgttgtc
     1441 gcgcaggtag tcgaagccga actcgttgtt ggtgccgtag gtgatgtcgg cggcataggc
     1501 ggcacgccgg tcggcctcgc cgatgccggg gacgatgcag ccgacggtga gcccgaggaa
     1561 ctcgtagatc tgccccatcc agcggctgtc gcgccgggcc aggtagtcgt tgacggtgac
     1621 caggtgcacg cccttgccgg gcagcgcatt gaggtaggcc gcgagcgtgg agacgagggt
     1681 cttgccctcg cccgtcttca tctcggcgat ggcaccctgg tgcaggaaca tgccgcccag
     1741 gagctgcacg tcgaaatgcc gctggccgag cgtgcgcttg gcggcctcgc gcaccgtggc
     1801 gaaggcctcg gggaggagcg cgtcgagcgt ctcgccggcg gccagccggc tgcggaactc
     1861 ctcggtcttg gccctgatcg ccgcgtcgtc gagcgcctcg aaggcgggct ccaggccatt
     1921 gatctcgtcg actgtcttgc gggcggactt gaccaggcga tcgttggtcg tgccgaagag
     1981 ctttcgggcg atggcgccga gcatcacggt atgtggcctc acggtttcgg ccgtgcggaa
     2041 cggcggcccc gcacggccat tgcagcctgt cagataaggt cggcccagag gtgtgtcaac
     2101 gaaaccacaa atgcggcacg tcgc
//
LOCUS       DAOTFI010000415         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_62664_length_2102_cov_8.733268, whole genome shotgun sequence.
ACCESSION   DAOTFI010000415 DAOTFI010000000
VERSION     DAOTFI010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62664_length_2102_cov_8.733268"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="P5558_23920"
     CDS             complement(<1..1193)
                     /locus_tag="P5558_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HRY27432.1"
                     /translation="MRLTNGLRQRAMDGNPIGVGLIGCGKFATMFLAQAARTPGIAIR
                     AIADLDPAGARAKVERSTGLDADEIHFVDNHASVVHDDDVELVIEATGDPVQGVVHAL
                     AAIEARRHILMVNVEADVLVGPALAKRARHRGVVYSLAYGDQPALVVELVDWAHAAGF
                     EIVAAGKGTKHRPHFRFSTPETVWDHYGLTPERAAASGMNPKMFNSFLDGTKSAIEMA
                     AIANATDLEVAPDGLLFPPAATGDLATVLSQRAASAGMNGHGMVETVSCLDGEGRPVP
                     NDLRWGVYVVFKAASDYVAGCFRDYGLVTDPSGRYATLWRPCHLIGLELGISVADMAL
                     RGEPTGAPWRWAGDVAATAKRDLEVGETLDGEGGFTVYGALVPAAVAAAEGLLPIGLA
                     HHARL"
     gene            complement(1468..>2102)
                     /locus_tag="P5558_23925"
     CDS             complement(1468..>2102)
                     /locus_tag="P5558_23925"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HRY27433.1"
                     /translation="DLDDCGPMVLDALIKIKAETDSTLTFRRSCREGICGSCSMNIDG
                     HNTLACTKAIEDVAGDDVTVYPLPHMSVIKDLVVDFTQFWAQYESIEPWLKTYSTDPA
                     KERLQSVNDREELDGLYECILCACCSTACPSYWWNSDRYLGPAVLLQAYRWIADSRDE
                     ATGERLDELEDPFKLYRCHTIMNCTNTCPKGLNPARAIGEIKKLVVERRI"
BASE COUNT          336 a          695 c          729 g          342 t
ORIGIN      
        1 ctgaggcgcg catggtgggc gaggccgatc ggcagcaggc cctcggcggc cgcgacggcg
       61 gccgggacca gggcgccata gacggtgaag ccgccctcgc cgtcgagcgt ctcgccgacc
      121 tcgagatcgc gctttgccgt cgccgccacg tcgccggccc agcgccaggg ggcgccggtc
      181 ggctcgccgc gcagcgccat gtcggcgacc gagatgccga gctcgaggcc gatcaggtgg
      241 cagggccgcc agagcgtcgc gtaccggccg ctcgggtcgg tcacgaggcc gtagtcccga
      301 aagcagccgg cgacgtagtc gctcgccgcc ttgaagacga catagacgcc ccagcgcagg
      361 tcgttcggca ccggccggcc ttcgccgtcg aggcaggaga ccgtctcgac catgccgtgg
      421 ccgttcatgc cggcggatgc ggcccgctgg ctcagcacag tggcgaggtc gccggtcgcg
      481 gccggcggga agagcagccc gtcgggcgcc acctcgaggt cggtggcatt ggcgatcgcc
      541 gccatctcga tcgccgactt ggtgccgtcg aggaaggaat tgaacatctt cgggttcatg
      601 ccggaagcgg cggcgcgctc gggggtgagc ccgtagtgat cccagacggt ctcgggcgtc
      661 gagaagcgga agtgcggccg atgcttcgtg cccttgccgg cggcgacgat ctcgaatcct
      721 gcggcatgcg cccagtcgac cagctcgacg accagggccg gctggtcgcc ataggccagg
      781 ctgtagacca cgccgcgatg ccgcgcccgc ttggccagcg ccgggccgac cagcacgtcg
      841 gcctcgacat tgaccatcag gatgtggcgc cgcgcctcga tcgcggccag cgcatggacc
      901 acgccctgca ccgggtcgcc ggtcgcctcg atcaccagct cgacatcgtc gtcgtggacg
      961 accgaggcgt ggttgtcgac gaagtggatc tcatcggcat cgaggccggt cgaacgttcg
     1021 accttcgccc tggcacccgc gggatcgagg tcggcaatgg cgcgaatcgc gatgcccggc
     1081 gtgcgggccg cctgggccag gaacatggtg gcgaacttgc cgcagccgat cagcccgacg
     1141 ccgatcgggt tgccgtccat cgcccgctgc cgcaggccat tcgtcagacg catgcggtcc
     1201 ccctgatgcc ctgacgccgc acgcgacccg gcgccatgct gtgtcgtgca actgcgaggg
     1261 gatggtcgcc gcgttgtcgc ggtctgtcca tacgtttcgc gccgccgcgc cgccgagagg
     1321 gggcggtcag gaattcggca caaaaaaaag gaggctgatt gcctcccttt tcagatcgga
     1381 cctgcgtgtg tgggcttacg cctcatttta tgcagaggac acgtaccaac acccaacacc
     1441 gccggctaga cgcgaaaaaa gtgaaaatca gatgcgtcgc tcaaccacca gtttttttat
     1501 ctcgccgatc gccctggccg ggttgaggcc cttcgggcag gtgttggtgc agttcatgat
     1561 ggtatggcag cggtacagct tgaatggatc ctcgagctcg tcgagccgct cgcccgtggc
     1621 ctcgtcgcgg ctgtcggcga tccagcgata ggcctgcagc agcaccgccg gacccagata
     1681 cctgtcgctg ttccaccagt aggaggggca ggcggtcgag cagcaggcgc agaggatgca
     1741 ctcgtagagc ccgtccagct cctcgcggtc gttcaccgac tgcaggcgct ccttcgcggg
     1801 atcggtcgag taggtcttga gccaaggctc gatcgattcg tactgcgccc agaactgggt
     1861 gaagtcgacg accaggtcct tgatcaccga catgtgcggc agggggtaga ccgtcacgtc
     1921 gtcgccggcg acgtcctcga tcgccttggt gcaggcgagc gtgttgtgcc cgtcgatgtt
     1981 catcgagcag gagccgcaga tgccctcgcg gcaggagcgg cggaaggtga gggtgctgtc
     2041 ggtctcggcc ttgatcttga tcagcgcatc gagaaccatc ggcccgcaat cgtcgagatc
     2101 ga
//
LOCUS       DAOTFI010000416         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_63226_length_2088_cov_5.783571, whole genome shotgun sequence.
ACCESSION   DAOTFI010000416 DAOTFI010000000
VERSION     DAOTFI010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63226_length_2088_cov_5.783571"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="P5558_23930"
     CDS             complement(<1..441)
                     /locus_tag="P5558_23930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY27434.1"
                     /translation="MKKSARTLLLASALVAVALPAIAQQRLVINSFGGVYEENHRRLV
                     IEPFEQMHNVTIEVVTLYSADTLAQLRAQKDNPQFDVVHFSGGQEVVAAAEGLLVPIQ
                     PSELTNYDQLYPIAVEGIEKGHGPVYSISVAGLLYDETRIDPAPT"
     gene            complement(467..940)
                     /locus_tag="P5558_23935"
     CDS             complement(467..940)
                     /locus_tag="P5558_23935"
                     /inference="COORDINATES: protein motif:HMM:NF024307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HRY27435.1"
                     /translation="MARSLYVEFTEARVRSIITLNEDNAPVTCATIWEALVRPIRSPA
                     LHAMFAGPEIMLGLPPEAQTFDAAAMPAENQDCFPKAGECLWFYQRKNLMKGLADELW
                     EIGIFYGDGGRVFGPLGWTPVNIFGRITENLEGFQAECAKIRWEGAKTVELGRNA"
     gene            complement(1043..>2088)
                     /locus_tag="P5558_23940"
     CDS             complement(1043..>2088)
                     /locus_tag="P5558_23940"
                     /inference="COORDINATES: protein motif:HMM:NF016201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HRY27436.1"
                     /translation="TSATRAGETISSFKTRADRVRAYLEDPSIGTDAVERLLDAAHAL
                     SMNRSRNQAIRQLSRREQADRLHEEAAGPHDEFAHLVQRAERPTPNLDRVPLEPDQNL
                     LLFIRDHSPDIAAWEADLLTIVDEEARYFIPQIETKIMNEGWASYWHHKIMMSLDLPQ
                     AIRLEFMVRHNQVICPHPGSLNPYHLGFVVWHDIERRFGGADSKEGRAKLFEVRESDR
                     DTAFLRRFLTPELMRELDLFSYKPRGEHLVVDRVSDEAHWEEVKAKLLAQIGTGSLPV
                     ISVIDADHAGRRALMLRHEHDGRDLDMTSAEKTLQHVRTLWRHDVYLQTRLRGKKALM
                     SSTEAGFDARLVA"
BASE COUNT          304 a          653 c          760 g          371 t
ORIGIN      
        1 cgtgggcgcg gggtcgatcc gggtctcgtc gtagagcagg cccgccaccg agatggagta
       61 gaccggcccg tggcccttct cgatgccctc gacggcgatc gggtagagct ggtcgtagtt
      121 ggtgagctcg gagggctgga tcggcacgag caggccctcg gccgcggcca cgacctcctg
      181 gccgcccgag aagtggacga cgtcgaactg cgggttgtcc ttctgggcac ggagctgcgc
      241 cagggtgtcg gcggagtaga gggtgacgac ctcgatcgtc acgttgtgca tctgctcgaa
      301 cggctcgatg acgaggcggc ggtggttctc ctcgtagacg ccgccgaagg agttgatgac
      361 gaggcgctgc tgggcgatgg ccggcagggc gacggcgacc agggcggaag cgaggaggag
      421 cgtgcgagcc gacttcttca tggtgcgttt ccttcgcgtg tgctggtcag gcgttgcggc
      481 cgagctcgac cgtcttcgcc ccttcccagc ggatcttcgc gcattcggcc tggaagccct
      541 cgaggttctc ggtgatgcgg ccgaagatgt tgaccggcgt ccagccgagc ggcccgaaga
      601 cgcggccgcc gtcgccgtag aagatgccga tctcccagag ctcgtcggcg aggcccttca
      661 tcaggttctt gcgctggtag aaccacaggc actcgccggc cttggggaag cagtcctggt
      721 tctcggccgg catggcggcg gcatcgaagg tctgcgcctc gggcggcagg cccagcatga
      781 tctcgggccc tgcgaacatc gcgtgcaggg ccggggagcg gatcggccgc accagcgctt
      841 cccagatcgt cgcacaggtg acgggcgcgt tgtcctcgtt cagcgtgatg atcgaacgaa
      901 cgcgggcttc cgtgaactcg acgtagagtg agcgcgccat gatgccgacc atcccccctt
      961 atcgctgcat tctgccactg cgaggattgc cagtcgcacc gcggcttgtc caacgggaag
     1021 ttccgttacg gccgtgcccc gatcaggcga cgagcctggc gtcgaagccg gcctcggtcg
     1081 agctcatgag cgccttcttg ccgcgcagcc gggtctgcag atagacgtcg tggcgccaga
     1141 gggtgcggac gtgctgcagg gtcttctcgg cactcgtcat gtcgaggtcg cggccgtcgt
     1201 gctcgtggcg cagcatcagc gcccgccggc cggcatggtc ggcgtcgatg acgctgatca
     1261 ccggcaggct gccggtgccg atctgggcga gcagcttcgc cttgacctcc tcccagtgcg
     1321 cctcgtcaga gacgcggtcg accaccagat gctcgccgcg gggcttgtag gagaagagat
     1381 cgagctcgcg cataagctcg ggggtcagga agcggcgcag gaaggccgtg tcgcggtcgc
     1441 tctcgcgcac ctcgaagagc ttggcgcggc cctctttcga atcggcaccg ccgaagcgcc
     1501 gctcgatgtc gtgccagacg acgaaaccca ggtggtaggg attgaggctg cccggatggg
     1561 ggcagatcac ctggttgtgg cggaccatga actcgagccg gatcgcctgg ggcaggtcga
     1621 gggacatcat gatcttgtgg tgccagtagc tggcccagcc ctcgttcatg atcttggtct
     1681 cgatctgcgg gatgaagtag cgcgcctcct cgtcgacgat ggtgagcagg tccgcctccc
     1741 aggcggcgat gtcggggctg tggtcgcgga tgaagagcag caggttctgg tccggctcga
     1801 gcggcacccg gtcgagattg ggagtcggtc gctcggctcg ctggaccaga tgggcgaatt
     1861 cgtcgtgcgg gccggccgct tcctcgtgca gccggtcggc ctgctccctg cggctgagct
     1921 ggcggatcgc ctggttgcgc gaccggttca tcgagagcgc gtgcgccgcg tccagcaggc
     1981 gctcgaccgc atcggtgccg atcgacgggt cctcgagata ggccctgacc cggtccgccc
     2041 gcgtcttgaa cgagctgatg gtctcgccgg cccgggtggc gctggtga
//
LOCUS       DAOTFI010000417         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_65012_length_2046_cov_8.155198, whole genome shotgun sequence.
ACCESSION   DAOTFI010000417 DAOTFI010000000
VERSION     DAOTFI010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65012_length_2046_cov_8.155198"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="P5558_23945"
     CDS             complement(<1..794)
                     /locus_tag="P5558_23945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY27437.1"
                     /translation="MNRYAGRTVLITGSGRGIGRATALRLAKEGAAVAVLDIDSEPAH
                     DVARKIADMGRTAVALVGDIAEDGAGERLVGEAEKALGPLDVLVNNAAMAGRGTLEAT
                     EPDGWDAEFAVTLRAAYNFCRAVLPGMAERGRGAIVSVASVNALSALANPAYSAAKAG
                     LLALTRQIAVEYGPRGIRANAVSPGTIHTDHPSWQARLARDPDVFEKLARWYPVGRVG
                     QPDDIAAAIAYLAADEAGFVNGTNLVVDGGLLAGSAQMIEVLTTGA"
     gene            complement(791..1981)
                     /locus_tag="P5558_23950"
     CDS             complement(791..1981)
                     /locus_tag="P5558_23950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27438.1"
                     /translation="MPRKGIFVYAWDLLDEGVATVADRLRAAGADSVALATSYHAGKF
                     VRPHGRSGKVYFPDDGTIYFRHRPERYGRVEPVPHPMLEDFDPLTALADEAPDLARVG
                     WTVCCHNSRLGRAYPELIAQNAVGDGLFYSLNPAHPDVREYVVTMVVDQAERYGLDAV
                     TLETPGWLPYEHGYHHEFALVPLDPWIGLLLGLCFAPESMAGARAAGIDAAGLKRRVV
                     SHIEAWCAADIAATPARAGEWQLADVVTDGELAAYLRWRCRVVADLVAEIRDRLPKAT
                     ELRIIPSVQRPSARSWYEGSDLAMLAEVCDAVEICAYEPSAAAVAADIFDVRRRVGDA
                     ARLFAILRPSHPDLGGGAEAAAAARALRQAGVEGLDFYNYGHWRLAALEHVRAAFAAW
                     EEEL"
BASE COUNT          295 a          720 c          753 g          278 t
ORIGIN      
        1 tcggcgccgg tggtcagcac ctcgatcatc tgcgccgagc cggcgagcag cccgccgtcg
       61 acgacgagat tggtgccgtt gacgaagccg gcctcgtcgg cggcgagata ggcgatggcc
      121 gcggcgatgt cgtccggctg gccgacccgg ccgaccgggt accagcgcgc cagcttctcg
      181 aagacgtcgg gatcgcgcgc gaggcgcgcc tgccagctcg ggtgatcggt gtggatggtg
      241 ccggggctca ccgcgttggc gcgaatgcct ctcgggccgt actcgaccgc gatctgccgg
      301 gtcagcgcca aaaggccggc cttggcggcg ctgtaggcgg gattggccaa agcgctcagg
      361 gcattgaccg aggcgacgct gacgatggcc ccccggccgc gctccgccat gccgggcagg
      421 acggcccggc agaaattgta ggcggcgcgc agcgtgacgg cgaactcggc atcccagccg
      481 tccggctcgg tcgcctcgag ggtgccgcgg ccggccatgg cggcgttgtt gaccagcacg
      541 tcgagcggcc cgagcgcctt ttccgcctct ccgaccaagc gctcgccggc gccgtcctcg
      601 gcgatgtcgc cgaccagggc cacggccgtc cgccccatgt cggcgatctt tcgggccacg
      661 tcgtgcgccg gctcgctgtc gatatcgagc acggcgacgg ccgcaccctc cttcgccagc
      721 ctgagcgccg tcgcccggcc gatgccacgc ccgctgccgg tgatcagcac ggttcgtcca
      781 gcgtaacggt tcaaagctcc tcctcccagg cggcgaaggc cgccctgaca tgctccaggg
      841 cggcgagccg ccagtggccg taattgtaga agtcgagacc ctcgaccccg gcctgccgca
      901 gggcgcgggc cgcggccgcg gcctcggcgc cgccgccgag atcggggtgc gacggcctga
      961 ggatggcaaa gagccgggcc gcgtcgccga cccgtcggcg cacatcgaag atgtcggccg
     1021 cgacggccgc cgccgagggc tcgtaggcgc agatctcgac cgcgtcgcac acctcggcca
     1081 gcatggcgag gtcgctgccc tcgtaccagc tccgggcgct cggccgctgc accgagggga
     1141 tgatgcgcag ctcggtcgcc ttgggcaggc ggtcgcggat ctcggcgacg aggtcggcca
     1201 ccacccggca gcgccagcgc agataggcgg cgagctcgcc gtcggtgacg acgtcggcga
     1261 gctgccattc gcccgcccgt gccggtgtcg cggcgatgtc ggcagcgcac caggcctcga
     1321 tgtgggatac cacgcggcgc ttcaggcccg ccgcgtcgat gccggcggcc ctggcgcccg
     1381 ccatgctctc gggcgcgaag cagaggccga gcaggaggcc gatccaggga tcgaggggca
     1441 cgagagcgaa ctcgtgatga tagccgtgct cgtagggcag ccagcccggc gtctccagcg
     1501 tcacggcatc gagcccgtag cgctcggcct ggtcgacgac catggtcacc acgtactcgc
     1561 gaacgtcggg atgggccggg ttgaggctgt agaagagccc gtcgcccacg gcgttctgcg
     1621 cgatcagctc cggatatgcc cggccgagcc gggaattgtg gcagcagacg gtccagccga
     1681 cgcgggcgag atcgggggcc tcgtcggcca gggcggtcag cgggtcgaaa tcctcgagca
     1741 tgggatgcgg caccggctcg acgcggccgt agcgctcggg gcgatggcgg aaatagatgg
     1801 tgccgtcatc ggggaagtag accttgcccg aacggccgtg cggccggacg aacttgccgg
     1861 cgtggtagga ggtggcgagc gccaccgaat cggcgccggc ggccctgagc cggtcggcca
     1921 ccgtggcgac tccctcgtcc agcaggtccc aggcgtagac gaagattccc ttgcgcggca
     1981 tggcgccccc cagtcgttaa cgggtgccag cctaggagcc tgtccgagaa atggccaccg
     2041 cctgca
//
LOCUS       DAOTFI010000418         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_65569_length_2033_cov_6.738119, whole genome shotgun sequence.
ACCESSION   DAOTFI010000418 DAOTFI010000000
VERSION     DAOTFI010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65569_length_2033_cov_6.738119"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="P5558_23955"
     CDS             <1..621
                     /locus_tag="P5558_23955"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRY27439.1"
                     /translation="GSGFAVTLDGLVLTNEHVVTGCGELRLRPPEGGLTVAAGLLALD
                     PTNDLALLQAPVSFAASAVFRDGRGIRAGDGVVVVGHPLRGLLADEASVTTGAVSALA
                     GLRNDARYLQLTAPVQPGNSGGPLVDMSGRVVGVVVAKLDAMKVADATGDIPQNVNFA
                     VKAAIARSFLDANDVAYASAPAEGAMSAADVGAAAKAMTVLVECWR"
     gene            complement(618..>2033)
                     /locus_tag="P5558_23960"
     CDS             complement(618..>2033)
                     /locus_tag="P5558_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRY27440.1"
                     /translation="SAETMKKSVAYLEPFMEKADGQQKGTIVLATVKGDVHDIGKNLV
                     DIILTNNGYRCVNLGIKQPLGNILEAAREHRADAIGMSGLLVKSTVVMRENLEEMGRD
                     GLELPVILGGAALTRAYVEEDCVKAYDSGRVAYARDAFDGLKLMDTIAKGGFDSYLAD
                     IKEKQQQKKRPGRKQRTLEHNDGSMPLTRPVDMAETRLRRDQLANGVAVPPPPFWGSQ
                     IIQSVPVRALLPYLNDNMLYQFHWGYKKEGRSLPEFLAWARKELRPVLQDLVAEAEAE
                     EVFLPQAAYGYWKAAGEGNDVVLFDEAGTTELCRFTLPRQAKAGGLCIADFFRDIDSD
                     ERDVIGLQVVTVGQRAAEVAREWFKADRYQDYVRLHGLGVELAEALAEYVHAKIRAEL
                     GFGHEDAREMAQVLKQGYRGSRYSFGYPACPTLKDQEPLLELLGSSRIGVELSEGWQL
                     HPEQSTSAIVLHHPQARYFNI"
BASE COUNT          277 a          711 c          699 g          346 t
ORIGIN      
        1 ggctcgggct tcgccgtgac cctggacggg ctggtgctga ccaacgagca tgttgtcacc
       61 ggttgcggcg agctgcggct ccgcccgccc gagggcggcc tgacggtggc ggccgggctg
      121 ctcgccctcg acccgaccaa cgacctcgcc cttctccagg cccccgtgag cttcgccgcg
      181 agcgctgtct ttcgcgacgg gcggggcatc cgggcgggcg acggcgtcgt cgtcgtcggc
      241 catccgctcc gcggcctgct cgccgacgag gcttccgtca ccaccggtgc cgtgagcgcc
      301 ctcgccggcc ttcgcaacga cgcccgctac ctgcagctca ccgcgcccgt gcagccgggc
      361 aacagcggtg gcccgctggt cgacatgagc ggccgggtgg tcggcgtcgt cgtcgccaag
      421 ctcgacgcga tgaaggtcgc cgatgccacc ggcgacatcc cgcagaacgt caacttcgcc
      481 gtgaaggcgg cgatcgcccg gagcttcctc gacgccaacg acgtcgccta tgcgtcggca
      541 cccgccgaag gggccatgag cgccgccgat gtcggcgccg ccgcgaaggc catgaccgtg
      601 ctggtggagt gctggcgcta gatgttgaag tatcgggcct gcgggtggtg gaggacgatg
      661 gcgctggtcg actgctcggg gtggagctgc cagccctcgc tgagctcgac cccgatcctg
      721 gacgagccga gcagctcgag cagcggctcc tggtccttga gcgtcgggca ggcgggatag
      781 ccgaagctgt agcgggagcc gcgatagccc tgcttgagga cctgcgccat ctcgcgcgcg
      841 tcctcgtggc cgaagccgag ctcggcccgg atcttggcat ggacgtactc ggccagcgcc
      901 tcggccagct cgacgccgag gccgtgcagg cggacatagt cctgatagcg gtcggccttg
      961 aaccactccc tggccacctc ggccgcccgc tggccgacgg tcaccacctg caggccgatg
     1021 acgtcgcgct cgtcgctgtc gatgtcgcgg aagaaatcgg cgatgcagag cccgcccgcc
     1081 ttggcctgcc tgggcagcgt gaagcggcag agctcggtcg tgccggcctc gtcgaagagc
     1141 accacgtcgt tgccctcgcc cgccgccttc cagtagccgt aggcggcctg cggcaggaag
     1201 acctcctcgg cctcggcctc ggcgacgagg tcctggagga ccgggcgcag ctccttcctg
     1261 gcccaggcga ggaactcggg gagcgagcgc ccctccttct tgtagcccca gtggaactgg
     1321 tagagcatgt tgtcgttgag gtacggcagg agcgccctga ccggcacgct ctggatgatc
     1381 tgggatcccc agaagggcgg cggcggcacc gccacgccgt tggcgagctg gtcgcggcgc
     1441 agcctggtct cggccatgtc gaccggccgg gtgagcggca tcgagccgtc attgtgctcg
     1501 agcgtgcgct gcttcctgcc cggccgcttc ttctgctgct gcttttcctt gatgtcggcg
     1561 aggtagctgt cgaacccgcc tttggcgatg gtgtccatca gcttcagccc gtcgaacgca
     1621 tcgcgggcat aggccaccct gccggagtcg taggccttga cgcagtcctc ctcgacatag
     1681 gcccgggtga gcgcggcacc gcccaggatc accggcagct cgagcccgtc ccggcccatc
     1741 tcctcgaggt tctcgcgcat cacgacggtc gacttgacca agaggccgga catgccgatc
     1801 gcgtccgcgc ggtgctccct ggccgcctcc aggatgttgc ccaagggctg cttgatgccg
     1861 agattgacgc agcgatagcc gttgttggtg aggatgatgt cgaccaggtt cttgccgatg
     1921 tcgtggacgt cgcccttgac cgtggcgagc acgatcgtcc ccttctgctg gccgtcggcc
     1981 ttctccatga agggctcgag ataggccacc gacttcttca tggtctcggc cga
//
LOCUS       DAOTFI010000419         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_67445_length_1991_cov_5.190083, whole genome shotgun sequence.
ACCESSION   DAOTFI010000419 DAOTFI010000000
VERSION     DAOTFI010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67445_length_1991_cov_5.190083"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="P5558_23965"
     CDS             complement(<1..844)
                     /locus_tag="P5558_23965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27441.1"
                     /translation="MSIESLREQLFLTAEIEAERFLAILRLTAVLVLALALGAALLAV
                     DGPPPAAQRQIVVAGSVMAVYAGIGLISYLTARPGRFRRWMPWLFTTADCALVLFNVA
                     TTVLNIGAPPATVWVFPAIWLAPLVLVFGSLHYRPWLQGYAAAVLVGGLVLLLAGLPQ
                     EGTLERILPLLDLPPTVVRIVLLGLAGLVLVLAAARRKALLERAIEEAVERTNLARYL
                     PPEIAALLAGGDVEALRRGWDVEIGILMVDIRGFTRRAEHLAPPELSTFLGTFRHHVI
                     EAAMA"
     gene            957..>1991
                     /locus_tag="P5558_23970"
     CDS             957..>1991
                     /locus_tag="P5558_23970"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY27442.1"
                     /translation="MVDYIIVGGGAAGCVLAARLSELPDASVLLLEAGPPDTDRLIHM
                     PVGFYRMTDGPLTWGFRTAPLAHADGREMVFPQGRVLGGGSSINAMVYTRGNAADYDA
                     WANEEGCDGWAYADVLPYFRKAEDNERLSDAWHGTGGPLGVSDQVSPHHLTRAFVRAC
                     QEAGIPYNPDFNGRDQAGAGLYQVTQRGGRRCSTAVGYLKAALARPNLELVTDCRVER
                     LIVEGGRAVGVAYNRYGRQAIARAESEVIVTAGAVGSPRLLLLSGIGPAEELRALGID
                     VVHDLQGVGRNFHDHIDAYVISELSGPHSYDGHTRPHRMLMAGLEYKLFGKGPVTSNL
                     AEGGAFVLTDP"
BASE COUNT          313 a          727 c          695 g          256 t
ORIGIN      
        1 gggccatcgc cgcctcgatg acgtgatggc gaaacgtgcc gagaaaggtc gagagctcgg
       61 gcggcgccag atgctcggcg cgccgggtga agcccctgat gtcgaccatc aggatgccga
      121 tctccacgtc ccagccgcgc cgcagcgcct cgacatcgcc gccggccagc agggccgcga
      181 tctccggcgg cagatagcgg gcgaggttgg tgcgctccac cgcctcctcg atggcccgct
      241 ccaaaagcgc cttgcgccgc gcggcggcga ggaccagcac caggccggcg aggccgagga
      301 gcacgatccg gacgaccgtc ggcggcaggt cgagcagcgg caggatccgt tcgagcgtgc
      361 cctcctgcgg caggccggcg agcagcagga ccagcccgcc gaccagcacg gccgccgcgt
      421 agccctgcag ccagggccgg tagtggagcg agccgaagac caggaccagg ggggcgagcc
      481 agatcgccgg gaaaacccag accgtcgccg gcggtgcgcc gatattgagc accgtggtcg
      541 ccacattgaa gaggacgagg gcgcagtccg cggtggtgaa gagccagggc atccagcggc
      601 gaaaccggcc gggccgggcg gtcagatacg agatcaggcc gatgcccgca tagacggcca
      661 tgacgctgcc cgccaccacg atctgccgct gggcggcggg cggcggcccg tcgaccgcga
      721 ggagggccgc cccgagcgcc agcgccagga ccagcaccgc ggtcagccgc aggatcgcga
      781 ggaaccgctc ggcctcgatc tcggccgtca gaaaaagctg ctccctcaga ctctcgatgc
      841 tcacctcggg cctttcgtgc cggcacctgc ccggcaccca tagcgccgct tcgccccgct
      901 tgtcatgccg ggcaccagcc cctaggcaac gacgagatgt ctgagggagg ggagtcgtgg
      961 tggattacat catcgtcggc ggcggtgccg cgggctgcgt gctcgccgcc cggctctccg
     1021 agctgcccga cgcctcggtg ctgctgctcg aggccgggcc gcccgacacc gaccggctga
     1081 tccacatgcc ggtcggcttc taccggatga ccgacggccc gctcacctgg ggtttccgca
     1141 cagccccgct cgcccatgcc gacggccgcg agatggtctt cccgcagggc cgcgtgctcg
     1201 gcggcggcag ctcgatcaac gccatggtct acacccgtgg caacgccgcc gactacgatg
     1261 cctgggccaa cgaggaaggc tgcgacggct gggcctatgc cgatgtgctg ccctatttca
     1321 ggaaggccga ggacaacgag cggctgagcg acgcctggca cggcaccggc gggccgctcg
     1381 gcgtctcgga ccaggtcagc ccgcatcatc tgacccgggc cttcgtgcgc gcctgccagg
     1441 aggccggcat tccctacaac cccgacttca acggcaggga ccaggccggc gccgggctct
     1501 atcaggtgac gcaacggggc gggcggcgct gctcgacggc ggtcggctac ctgaaggcgg
     1561 cgctggcccg gccgaacctc gagctcgtca ccgactgccg ggtcgagcgg ctcatcgtcg
     1621 agggtggccg agcggtcggc gtcgcctaca accgctacgg gcggcaggcg atcgcccggg
     1681 ccgagagcga ggtgatcgtg accgccggtg ccgtcggctc gccccgcctc ctgctcctct
     1741 ccggcatcgg cccggccgaa gagctgcgcg ccctcggcat cgacgtcgtg catgatctcc
     1801 agggcgtcgg ccgtaacttc cacgaccata tcgacgccta tgtcatcagc gagctgagcg
     1861 gcccgcactc ctatgacggc catacccgcc cgcaccggat gctgatggcc gggctcgagt
     1921 acaagctctt cggcaagggc ccggtgacct ccaacctcgc cgagggcggg gccttcgtgc
     1981 tgaccgaccc c
//
LOCUS       DAOTFI010000420         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_68488_length_1969_cov_7.402299, whole genome shotgun sequence.
ACCESSION   DAOTFI010000420 DAOTFI010000000
VERSION     DAOTFI010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68488_length_1969_cov_7.402299"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="P5558_23975"
     CDS             complement(<1..693)
                     /locus_tag="P5558_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRY27443.1"
                     /translation="MFKTTIKEMDWHGRRLRLESGRIARQADGAVLAQLGETVVLATA
                     VGARSIKPGQDFFPLTVNYQEKAFAAGKIPGGFFKREGRPTEKETLTSRLIDRPIRPL
                     FPAGFKNETQIVVTVLAHDGENDPDMLGMVAASAALTISGLPFLGPIGACRVGHINGQ
                     FVVNPTIEELKGSALDLVVAGTRDAVMMVESEAKELPEATMLDAVMHGHRAFQPVIDL
                     IIELAMACAKEPW"
     gene            complement(797..1066)
                     /gene="rpsO"
                     /locus_tag="P5558_23980"
     CDS             complement(797..1066)
                     /gene="rpsO"
                     /locus_tag="P5558_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRY27444.1"
                     /translation="MSITQERKLELIKSYAVKDGDTGSPEVQCAILSERIGNLTEHLK
                     AHQKDFASRRGLLMMVGQRRRLLDYLNRSDGDRYRQLIERLGLRR"
     gene            complement(1104..>1969)
                     /gene="truB"
                     /locus_tag="P5558_23985"
     CDS             complement(1104..>1969)
                     /gene="truB"
                     /locus_tag="P5558_23985"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRY27445.1"
                     /translation="PSGMTSTHVVNRVKRLTDAAKAGHGGTLDPLATGVLPLALGEAT
                     KTVMYVMDAPKRYRFTVRFGAATNTDDSEGEVIGTSGERPTDAAIALALKAFEGRIMQ
                     RPPDFAAVKVAGERAYDLARRGEALDLPPREVHFIELRMIERPDVDHAVFEARCGKGA
                     YIRALARDLGQALGCLGHVTALRRLEVGAFTADAAIDLDTLVEIVQDDSLPQALVSVS
                     TALADIPALAVTEPQAARLRSGHPIQIVPRLVVGEVVEGGTIQAVREGEVVALGRLVD
                     GELSPLRVFNR"
BASE COUNT          298 a          669 c          679 g          323 t
ORIGIN      
        1 ccagggctcc ttggcgcagg ccatggcgag ctcgatgatc aggtcgatga ccggctggaa
       61 cgcccggtgc ccgtgcatca cggcatcgag catcgtcgcc tcgggcagct ccttcgcctc
      121 ggattccacc atcatcaccg cgtcgcgcgt gccggccacg acgaggtcga gagcgctccc
      181 cttgagctcc tcgatcgtcg ggttgaccac gaactggccg ttgatgtggc cgacgcggca
      241 ggcgccgatc gggccgagga agggcaggcc gctgatcgtc agcgcggcgc tggcggcgac
      301 catgccgagc atgtcggggt cgttctcgcc gtcatgggcg aggacggtga cgacgatctg
      361 cgtctcgttc ttgaagccgg ccgggaagag cgggcgaatc gggcggtcga tcagccgcga
      421 ggtcagcgtc tccttctcgg tcggccgccc ctcgcgcttg aagaagccgc caggaatctt
      481 gccggcggcg aacgccttct cctggtagtt gaccgtgagc gggaagaaat cctggccggg
      541 cttgatcgac ctggcgccga cggccgtggc gagcacgacg gtctcgccga gctgcgccag
      601 cacggcgcca tcggcctggc gggcgatgcg gccgctttcc agacgcaagc ggcgcccgtg
      661 ccagtccatt tccttgatgg tcgtcttgaa cattcactta gcctttcatg tgcctgcaac
      721 ggcaggcgac cggtccgaca ccagatcgga ccggcccgtc ttcaatggcg gccgtcgagg
      781 ctgcccgttc ctgcattcaa cgacgcaggc cgagccgctc gatgagctgc cggtagcgat
      841 caccgtcgct gcggttcagg tagtcgagca ggcggcgccg ctggccgacc atcatcaaaa
      901 gaccgcgcct cgacgcgaag tccttctgat gcgccttgag gtgctcggtc aggttgccga
      961 tgcgctcgct gaggatggcg cactgcacct ccggcgagcc ggtgtcgccg tccttcacgg
     1021 cataggactt gatgagctcg agcttgcgct cctgcgtgat cgacatcgtc tggtccttct
     1081 ggaaggcgag gcgcggggcc tgctcaacgg ttgaaaacgc gcagcgggct cagctcgcca
     1141 tccaccagcc gtccgagagc gacgacctcg ccctcccgga ccgcctggat ggtcccacct
     1201 tccaccacct cgccgacgac gaggcggggc acgatctgga tggggtgacc ggagcggagc
     1261 cgcgccgcct gtggctcggt cacggcgagc gccgggatgt cggccagcgc cgtcgaaacc
     1321 gaaaccaatg cttgcggtag gctatcatct tggacgattt cgacaagcgt gtcgagatcg
     1381 attgcagcgt ccgcggtgaa cgcccccacc tcgagccggc gcagcgccgt gacgtggccg
     1441 aggcagccca gcgcctggcc gaggtcgcgc gccagggccc ggatataggc ccccttgccg
     1501 caacgggcct cgaagacggc atggtcgacg tcgggccgct cgatcatgcg gagctcgatg
     1561 aagtggacct cgcgtggcgg caggtcgagc gcctcgccgc gacgcgccag gtcgtaggcg
     1621 cgctcgcccg ccaccttcac cgccgcgaaa tccggcggcc gctgcatgat ccggccctcg
     1681 aaggccttca gggccagggc gatcgcggca tcggtgggtc gctccccgga cgtcccgatg
     1741 acctcgccct cgctgtcgtc ggtgttggtc gcggcaccga agcggaccgt gaaccggtag
     1801 cgcttgggcg catccatgac atacatgacg gtcttggtcg cctcgccgag ggccagcggc
     1861 aaaacgcccg tggcgagcgg gtcgagcgtg ccgccatggc cggccttggc ggcgtcggtc
     1921 aggcgcttga cccgattgac cacgtgggtc gaggtcatgc cggacggct
//
LOCUS       DAOTFI010000421         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_68889_length_1961_cov_7.057188, whole genome shotgun sequence.
ACCESSION   DAOTFI010000421 DAOTFI010000000
VERSION     DAOTFI010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68889_length_1961_cov_7.057188"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="P5558_23990"
     CDS             complement(<1..762)
                     /locus_tag="P5558_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRY27446.1"
                     /translation="MTEITPELLQRHGLTGDDLARIEEILGRAPTLVELGIFSVMWSE
                     HCSYKSSKIHLGKLPTTAPWVVQGPGENAGVIDIGGGQVAIFKMESHNHPSFIEPYQG
                     AATGVGGILRDVFTMGARPVALLNALRFGAADHSRTRHLVAGVVAGIGGYGNCVGVPT
                     VGGECTFHHAYNGNILVNAMAVGIADADKVFYASAGGIGNPVLYVGAKTGRDGIHGAT
                     MASAEFSETKEAERPTVQVGDPFTEKLLIEACLELM"
     gene            complement(759..1421)
                     /gene="purQ"
                     /locus_tag="P5558_23995"
     CDS             complement(759..1421)
                     /gene="purQ"
                     /locus_tag="P5558_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRY27447.1"
                     /translation="MRAAVVTFPGSNCDRDLAVALEAVLQRPVARVWHGDSELPPVDL
                     VGIPGGFAYGDYLRCGAIAARSPIMAAVARHAEAGGAVIGVCNGFQVLTEAGLLPGAL
                     IRNAGLRFVCRHVRVAVAARRNPFTDGYDEGAVLSLPVAHHDGNYVIDEAGLARLEAE
                     DRIAFRYLDNPNGSTRDIAGVLSANRRVLGLMPHPERAIEPALGSADGRPLFTSLLAA
                     LA"
     gene            complement(1525..>1961)
                     /locus_tag="P5558_24000"
     CDS             complement(1525..>1961)
                     /locus_tag="P5558_24000"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY27448.1"
                     /translation="IADELVAVFLGEGMERRAVASALDIQEAMAGLLREHPEWDLHVG
                     IGISAGEVVMGAMGARERMDFTVLGDPVNLASRLCDAAPPRSILVSEPVRIAVAGDPE
                     LVLEPLEPIRIKGKRELVPVFKALRAVRADRGEAPAIDRRAS"
BASE COUNT          288 a          680 c          717 g          276 t
ORIGIN      
        1 catcagctcg aggcaggcct cgatgagcag cttctcggtg aaggggtcgc ccacctgcac
       61 ggtcggccgc tcggcctcct tcgtctcgct gaactcggcc gacgccatgg tggcgccgtg
      121 gatgccgtcg cggccggtct tcgcccccac gtagagcacc gggttgccga tgccgccggc
      181 gctcgcatag aagaccttgt cggcgtcggc gatgccgacc gccatggcgt tgaccaggat
      241 gttgccgttg taggcgtgat ggaaggtgca ttcgccaccg accgtcggca cgccgacgca
      301 attgccgtag ccgccgatcc cggcgaccac cccggccacc aggtgccggg tcctggagtg
      361 gtccgcagca ccgaagcgga gcgcgttcag gagggccacc ggtcgggccc ccatggtgaa
      421 gacgtcgcgc aggatgccgc cgacgcccgt ggcggctccc tggtagggct cgatgaagga
      481 ggggtggttg tggctctcca tcttgaagat ggcgacctgg ccgccgccga tgtcgatcac
      541 cccggcgttc tcgcccggcc cctggaccac ccagggtgcg gtggtcggca gcttgccgag
      601 atggattttc gagctcttgt aggagcagtg ctcggaccac atgaccgaga agatgccgag
      661 ctcgaccagg gtcggggcgc ggccgaggat ctcttcgatc cgggcgaggt cgtcgcccgt
      721 cagcccgtgg cgctgcagca gctccggcgt gatctcggtc atgccagggc cgccagcagc
      781 gaggtgaaga gcggccggcc gtcggcgctg ccgagcgccg gctcgatcgc gcgctcggga
      841 tgcggcatca ggccgagcac gcggcggttg gccgagagca cgccggcgat gtccctggtc
      901 gagccgttcg ggttgtcgag atagcgaaag gcgatgcggt cctccgcctc gagcctggcg
      961 aggcccgcct cgtcgatgac gtagttgccg tcgtgatgcg ccaccggcag ggagagcacg
     1021 gcaccctcgt catagccgtc ggtgaagggg ttgcggcggg ccgccaccgc cacgcggaca
     1081 tggcgacaga cgaagcgcag gccggcgttc ctgatcaggg caccgggcag gagcccggcc
     1141 tcggtcagca cctggaagcc gttgcagacg ccgatgacgg caccgcccgc ctcggcatgg
     1201 cgggcgaccg ccgccatgat cggcgagcgg gcggcgatgg cgccgcagcg caggtagtcg
     1261 ccataggcga agccgcccgg aatgccgacc agatcgaccg gcggcagctc gctgtcgccg
     1321 tgccagacgc gcgccaccgg ccgctgcagc acggcctcga gcgctaccgc caggtcacgg
     1381 tcgcagttgg atcccggaaa ggtgacgacg gcggcgcgca tgcggggttc cgaaggcttc
     1441 gttcgccgcc gggcttaacc gccagccggg cgaggcgcaa gggcagggca tgcggcacct
     1501 ttcccggtgc ggtcgaaagc cttctcagga agcccggcgg tcgatcgccg gcgcctcgcc
     1561 cctgtccgcc cggaccgcgc gcagcgcctt gaagaccggc acgagctcgc gcttgccctt
     1621 gatccggatc ggctcgagcg gctcgaggac gagctccgga tcgcccgcca cggcgatgcg
     1681 gaccggctcg ctcaccagga tcgaccgggg cggggcggcg tcgcagaggc gggacgccag
     1741 gttgacgggg tcgcccagga ccgtgaagtc catgcgctcg cgggcaccca tggcccccat
     1801 caccacctcg ccggcgctga tgccgatgcc gacatgcagg tcccattcgg ggtgctcgcg
     1861 cagcagcccg gccatggcct cctggatgtc gagggcggag gcgacggcgc ggcgctccat
     1921 gccttcgccc aggaagaccg cgaccagctc gtcggcgatg a
//
LOCUS       DAOTFI010000422         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_69337_length_1951_cov_5.670886, whole genome shotgun sequence.
ACCESSION   DAOTFI010000422 DAOTFI010000000
VERSION     DAOTFI010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69337_length_1951_cov_5.670886"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="P5558_24005"
     CDS             <1..467
                     /locus_tag="P5558_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRY27449.1"
                     /translation="AFADQLAREIEGAKRLELHLAPPLLASRDPRTGRPEKRRYGPWI
                     LHVLRLLARLKGLRGTAWDPFGRTAERRLERQLIQDYETLLADLAGRLTPANHDVAVE
                     LASLPERIRGFGHVKERNVKEAKAAEAGLLRRLDIEATTPVRPKPAAASLSP"
     gene            complement(457..906)
                     /locus_tag="P5558_24010"
     CDS             complement(457..906)
                     /locus_tag="P5558_24010"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HRY27450.1"
                     /translation="MGELAHDLYDADFYAWTREQAAALRQLAAERQNLPIDASRLAEE
                     VEDLGKDQRNACRSQVRTIIEHLLKLRLSPATEPRAGWERSIMIARAALDDRLTGTLE
                     HDLEANLERLYRQAAALARQSLEAHGETDAAACIPERCPFTLDEIRG"
     gene            1096..>1951
                     /locus_tag="P5558_24015"
     CDS             1096..>1951
                     /locus_tag="P5558_24015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27451.1"
                     /translation="MSDDWRAAVDGAGDLAALKAVAGERAASGLSVREAERLARRLEA
                     LAPAPDLRVLYLGNHTLDLFARCVTGVALSRGVVVEGQVGPYDQHMQAVLDPAQGLAD
                     FDPHVVFLTLDLQRLLPVLPAGLAALSLKDRQGLVEEALGQVAAWVEAAKAATGATLL
                     IGNFWRPRRPHLGLADAKLGMGEAELYATLNARLFRDWSSDPQVFVVDVDQALATTGR
                     RAVHNARMAHLGKFAWAEAALPELAELLARHFATLVLPPKKCLVLDLDNTLWGGVLGE
                     EGPDGIRIA"
BASE COUNT          270 a          677 c          714 g          290 t
ORIGIN      
        1 gcgccttcgc cgaccagctc gcccgcgaga tcgagggggc gaagcggctc gagctccatc
       61 tggccccgcc gctgctcgcc agccgcgatc cgcgcaccgg ccgaccggaa aagcggcgct
      121 atggcccgtg gatcctccac gtgctccgcc tgctcgcccg gctgaagggc ctgcgcggca
      181 cggcctggga cccctttggc aggacggcag agcgtcggct ggaacggcag ctcatccagg
      241 actacgaaac cctgctcgcc gacctcgccg gccggctcac gccggcgaac cacgatgtcg
      301 ctgtcgagct cgccagcctg cccgagcgca ttcgcggctt cggccatgtg aaggagcgga
      361 acgtgaagga ggcgaaggcg gcggaggcgg gcctgctgcg gcggctcgac atcgaggcga
      421 cgacgcccgt gcgaccgaag ccagccgccg cgtcgctcag cccctgatct cgtcgagcgt
      481 gaaagggcag cgctcgggaa tgcaggccgc ggcgtccgtt tcgccatgcg cctcgaggct
      541 ctgccgggcg agggccgcag cctggcgata gagccgctcg agattggcct cgagatcgtg
      601 ctccagcgtg ccggtcagcc ggtcgtcgag ggcggcgcga gcaatcatga tgctgcgctc
      661 ccagccggcc cgtggctcgg tggcaggcga gagccggagc ttcaggagat gctcgatgat
      721 cgtgcggacc tggctgcggc aggcattgcg ctggtccttg cccaagtcct cgacctcctc
      781 cgcgagccgg ctggcgtcga tcgggagatt ctgccgctcg gccgcgagct ggcgcagggc
      841 cgcggcctgc tcccgcgtcc aggcgtagaa atccgcgtcg tagagatcgt gcgcgagctc
      901 gcccatcgac cacccctttc atccggcatg atagccgagg cggctgtcgc ccgccaccgg
      961 gcgaggggcg ccaacgctgt cttcgtccgc cggttgacgc aaccgccatt cgacgcttag
     1021 tggcggcacg acgagccttg ccgcgatggg gccggctctc gggcgatttc ccagaagaag
     1081 caagggcgag cggcaatgag cgacgactgg cgcgctgcgg tcgacggggc cggcgatctg
     1141 gcggccctga aggccgtggc cggcgagcgg gcggcgagtg ggctctcggt gcgcgaggcg
     1201 gagcggctgg cgcggcggct cgaggcgctg gcgccggcac cggacctgcg cgtgctctat
     1261 ctcggcaacc acacgctcga tctcttcgcc cgctgcgtca ccggtgtcgc actcagccgg
     1321 ggggtggtcg tcgaggggca ggtagggccc tatgaccagc acatgcaggc cgtgctcgat
     1381 ccggcccagg ggctggcgga cttcgacccg catgtcgttt tcctcacgct cgatctccag
     1441 cgattgctgc cggtgttgcc ggccgggctc gcggcgctgt cgctgaagga ccggcaggga
     1501 ctggtggagg aggcgctcgg gcaggtggcg gcctgggtgg aggccgccaa ggctgccacc
     1561 ggggcgaccc tgttgatcgg caatttctgg cggccgcgcc ggccgcatct cgggctcgcc
     1621 gatgccaagc tcggcatggg cgaggcggag ctatatgcca cgctgaacgc ccggctcttc
     1681 cgggactggt cgagcgaccc gcaggttttc gtcgtcgatg tcgaccaggc gctagcaacg
     1741 accggcaggc gggcggtgca caacgcccgc atggcccatc tcggcaagtt cgcctgggcg
     1801 gaggccgccc tgccggagct ggctgagctg ctggcccgcc atttcgccac cctcgtcctg
     1861 ccgccgaaga aatgcctcgt cctcgacctc gacaacacgc tctggggtgg cgtgctcggc
     1921 gaggaggggc cggacggcat ccgcatcgcc c
//
LOCUS       DAOTFI010000423         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_69902_length_1940_cov_6.427056, whole genome shotgun sequence.
ACCESSION   DAOTFI010000423 DAOTFI010000000
VERSION     DAOTFI010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69902_length_1940_cov_6.427056"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..484
                     /locus_tag="P5558_24020"
     CDS             305..484
                     /locus_tag="P5558_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27452.1"
                     /translation="MPETEGLGRTGWAALLVLVVGIVAVLVFGFAAAIYVGLLLTFAA
                     LVIMVMLCQGEPASE"
     gene            complement(521..1483)
                     /gene="thyX"
                     /locus_tag="P5558_24025"
     CDS             complement(521..1483)
                     /gene="thyX"
                     /locus_tag="P5558_24025"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRY27453.1"
                     /translation="MTLSAEAAAEIAAQRATEAPTRRAVVPALEALLYEPMPVLDQGF
                     VRVVDYMGDDAAVVQAARVSYGKGTKHVSNDRGLINYLMRHRHTTPFEMCEIKLHVKL
                     PIFVARQWIRHRTASVNEYSARYSVLDDGSYRPELAQLAAQAVTNRQGRGAPLDPAAA
                     QAVRDRLDEATASAEATYRWLLGDPEGAEAEGGERIDAEIGPGLSRELARMVLPLASY
                     TQWYWKIDLHNLLHFIALRADPHAQHEIRAYAEVLARIVEQWVPLTAAAFRDYRVEGA
                     ALSGKGLQVVRRMLAGETVDAAGSGLAAREWRELRILLGLPAED"
     gene            1541..1877
                     /gene="ssrA"
                     /locus_tag="P5558_24030"
     tmRNA           1541..1877
                     /gene="ssrA"
                     /locus_tag="P5558_24030"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
BASE COUNT          297 a          637 c          681 g          325 t
ORIGIN      
        1 gagcgtgcgg aagttgtcgc caccgctgca ggaggccagc atgagcgctg cgcctgccgt
       61 taccataact acacgtgaga gacgcattgg ccccctcctc ggttatccta ccggccggag
      121 attacaagac gtgcgggagt tgtacagcta cgccataccg aatatcgcgc agtgtggcgt
      181 tgctgccgta ccccaatggc cagtggcggc cagagtgcgg caagttctgt cgctgacaag
      241 ctcgaccgac cggtctagcc tagccgcaac ctggttcgtg taaaatggga aaggatcgaa
      301 gccgatgccg gaaaccgaag gtctcggccg caccggttgg gcagccctgc tggtgctcgt
      361 ggtgggcatt gtcgccgtcc tggtcttcgg cttcgcggcc gcgatctatg tcggcctcct
      421 gctgaccttc gcggcgctcg tcatcatggt catgctctgc cagggcgagc ccgccagcga
      481 atgagccggc cggccccggc cggcccggac cggcgcccgc tcagtcttcc gcaggcaggc
      541 cgagcaggat cctgagctcg cgccattcgc gggcggcgag gccggagccg gcggcgtcga
      601 cggtctcgcc tgcgagcatt cgtcgcacga cctgcaggcc cttgccggaa agggccgccc
      661 cctcgacccg gtagtcgcga aaggcggccg ccgtcagcgg cacccattgc tcgacgatcc
      721 gggccagcac ctcggcatag gcgcggatct cgtgctgggc gtgcggatcg gcgcggagcg
      781 cgatgaagtg cagcaggttg tgcaggtcga tcttccagta ccactgggtg tagctggcga
      841 gcggcaggac catgcgggcg agctcgcggc tgaggccggg gccgatctcg gcgtcgatcc
      901 tctcgccacc ttccgcctcg gccccctcgg ggtcgccgag cagccagcgg taggtcgcct
      961 cggcgctggc cgtggcctcg tccagccggt cgcgcaccgc ctgggcagcg gccggatcga
     1021 gcggcgcccc ccggccctgc cggttggtca ccgcctgggc cgcgagctgg gcgagctcgg
     1081 gccggtagct gccgtcgtcg agcaccgagt agcgcgccga gtactcgttg acgctggcgg
     1141 tgcggtggcg gatccactgc cgggcgacga agatcggcag cttcacgtgc agcttgatct
     1201 cgcacatctc gaagggcgtc gtgtggcggt gccgcatcag gtagttgatc aggccccggt
     1261 cattgctgac gtgcttcgtg cccttgccgt aggagacgcg ggccgcctgc acgacggccg
     1321 catcgtcgcc catgtagtcg acgacgcgca cgaagccctg gtcgagcacg ggcatgggct
     1381 cgtagagcag cgcctcgagc gccggcacca cggcgcggcg ggtcggcgcc tcggtcgccc
     1441 tttgggcggc gatctcggct gcggcttcgg cggaaagggt catggcgaca caccaggata
     1501 tggttcgggc tgccggatgc ggccggccgg gctctggtcg cctctttgaa agcgcttggc
     1561 aagcacctat atccagggag ccggagcgat ccggctatgg tgataaacgc cattcggaat
     1621 aagcgtttcg gacccggggg cagttcccgg cgcctccacc aactctctat ccgccattcg
     1681 tcggttgtcg acggacgtgc ggtgagcatg ggggcgaatc aggatcgacg agcgtggtaa
     1741 agggatggtt tttgcccggc atggtaccgc cgttaccgga ccgtttgtac aagtgccaac
     1801 gacaaccgta tggctctcgc tgcctagtct ctaggtaagc gcggctggca ggggccgggc
     1861 aacagaatcc tgccaaaaag tccagggagc ttcggcaccc tggcgcggcc cggggggcgc
     1921 cgggcaacag aagcccccca
//
LOCUS       DAOTFI010000424         1915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_71201_length_1915_cov_7.483333, whole genome shotgun sequence.
ACCESSION   DAOTFI010000424 DAOTFI010000000
VERSION     DAOTFI010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71201_length_1915_cov_7.483333"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="P5558_24035"
     CDS             complement(<1..523)
                     /locus_tag="P5558_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY27454.1"
                     /translation="MLANDQLAAWDREAFFHPSTHLASHARGDSAVRVITGGSGVWIE
                     DRDGKRLLDAFAGLYCVNVGYGRTEIADAIAAQAKQLAYYHAYVGHGTEASIRLARMI
                     LDRAPEGMSKVYFGLGGSDANETNVKLIWYYNNILGRTAKKKIISRWRGYHGSGLMTG
                     SLTGLGLFHRKFDL"
     gene            complement(569..>1915)
                     /locus_tag="P5558_24040"
     CDS             complement(569..>1915)
                     /locus_tag="P5558_24040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRY27455.1"
                     /translation="DGANLGEVASLSGAQSAQSVDAADAAFAGWATTLPQERARLLRR
                     WHELLLAAREDLARIMTLEQGKPLSEARGEIDYAASFVEFYAEETRRPNIEGVTSHLP
                     DAEVELWREPVGVAALVTPWNFPCAMITRKAAAALAAGCTVLVHPSHETPFSALALAE
                     LADRAGFPAGVFNVVTGQAPVVVEPWLGDPRVRALSFTGSTEIGRLLYRRSADTVKRL
                     VLELGGHAPFIVFADADLERAVDEAIKAKFATTGQDCLGANRLLVERPVYEAFCARFA
                     ERAAALTLGPGLDDRDLGPLMNEGAVKKQEAHVADALARGARCLTGGTRSPLGPLYYP
                     ATVLADVPTDALVFEEETFGPVAAIAPFDTEAEALQVANATEYGLVAYLHTQDPRRIY
                     RLSRALQFGMVAVNRTKVTGAPIPFGGMKQSGLAREGARQGLEAFTDVKYVCRDWA"
BASE COUNT          289 a          623 c          713 g          290 t
ORIGIN      
        1 gcaggtcgaa cttccggtgg aagaggccga gcccggtgag cgagccggtc atcaggcccg
       61 agccgtgata gccgcgccag cgcgagatga tcttcttctt cgcggtccgg ccgaggatgt
      121 tgttgtagta ccagatcagc ttgacgttgg tctcgttggc atcggagccg ccgaggccga
      181 aatagacctt cgacatgccc tccggcgccc ggtccaggat catcctggcg aggcggatcg
      241 aggcctcggt gccgtggccg acataggcgt ggtagtaggc gagctgcttc gcctgggccg
      301 cgatcgcatc ggcgatctcg gtccggccat agccgacatt gacgcaatag agcccggcga
      361 aggcgtcgag caggcgcttg ccgtcacgat cctcgatcca cacgcccgag ccgccggtga
      421 tcacccggac ggcgctgtcg ccgcgcgcat gggatgccag atgggtcgag ggatgaaaga
      481 aggcctcgcg atcccaggcg gcgagctgat cgttggcgag cattttgttc cctttgtcga
      541 gacatggcag gcgttggcgg cggtcgggtc aggcccagtc gcggcagaca tatttgacgt
      601 cggtgaaggc ctcgagtccc tgcctcgcac cctcgcgggc gaggccggac tgcttcatcc
      661 cgccgaaggg gatgggtgcc cctgtgacct tggtccggtt gacggcgacc atgccgaact
      721 gcagggcgcg gctgaggcgg tagatgcgac gcgggtcctg ggtgtggagg tacgccacga
      781 gcccgtactc ggtggcattg gcgacttgga gggcctcggc ctcggtgtcg aagggtgcta
      841 tggccgccac cggcccgaag gtctcctcct cgaacaccag cgcatcggtc ggcacgtccg
      901 ccagcacggt tgccgggtag tagagtggcc ccaggggcga gcgcgtaccg cccgtgaggc
      961 agcgggcacc gcgcgccagc gcgtcggcga cgtgcgcctc ctgcttcttg accgcgccct
     1021 cgttcatcag cgggccgagg tcgcggtcgt cgagcccggg gcccagcgtc agggcggcgg
     1081 cgcgctcggc gaagcgggcg cagaatgcct cgtagaccgg tcgctcgacg agcaggcggt
     1141 tggcgccgag gcagtcctgg ccggtggtcg cgaatttcgc cttgatcgcc tcgtcgacgg
     1201 cgcgctccag atcggcgtcg gcgaagacga tgaagggcgc gtggccacca agctcgagca
     1261 cgaggcgctt taccgtgtcg gccgagcgcc ggtagagcag ccggccgatc tcggtcgagc
     1321 cggtgaagga aagggcgcgc acccttggat cgccgagcca gggctcgacc acgaccggtg
     1381 cctggccggt gacgacgttg aaaacgcccg ccgggaagcc ggcccggtcc gccagctcgg
     1441 ccagggcgag ggccgagaac ggcgtctcgt gcgaggggtg gacgagtacc gtgcagcccg
     1501 cagcgagcgc tgccgcggcc ttgcgggtga tcatggcgca ggggaagttc cagggcgtga
     1561 cgagggccgc cacgccgacc ggctcgcgcc acagctccac ctccgcatcg ggcagatgcg
     1621 aggtgacgcc ctcgatgttg ggccgcctgg tctcctccgc gtagaactcg acgaaggagg
     1681 cggcgtagtc gatctcgccc cgggcttcgg agagcggctt gccctgctcg agcgtcatga
     1741 tccgggcgag gtcctcgcgc gcggccagca ggagctcgtg ccagcggcgc agcaggcggg
     1801 cccgctcctg cggcagggtc gtggcccagc cggcgaaggc ggcatcggcg gcatcgacgg
     1861 actgagcgct ctgggcaccc gagaggctgg cgacctcgcc gagattcgca ccatc
//
LOCUS       DAOTFI010000425         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_71251_length_1914_cov_6.288327, whole genome shotgun sequence.
ACCESSION   DAOTFI010000425 DAOTFI010000000
VERSION     DAOTFI010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71251_length_1914_cov_6.288327"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..657
                     /gene="rplI"
                     /locus_tag="P5558_24045"
     CDS             100..657
                     /gene="rplI"
                     /locus_tag="P5558_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRY27456.1"
                     /translation="MQVILLERVPKLGQMGQIVNVKPGYARNFLIPKGKALRATETAL
                     GAFESRRAQLEAQNLERRNEAEEASTIIDGASVVILRQASEGSQLYGSVSPRDIADAF
                     TEQGITFTRQQIRLESPLKTLGIHSVTVALHPEVEVQVSVNIARSHEEAEIQAGKAAP
                     VLEEEAIDPESDEFTRGSGFDELGI"
     gene            739..1440
                     /gene="phaR"
                     /locus_tag="P5558_24050"
     CDS             739..1440
                     /gene="phaR"
                     /locus_tag="P5558_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRY27457.1"
                     /translation="MAPRKVEAAPSGGREEGGGAEGETRKGGKVAIVKKYANRRLYNT
                     ASSSYVTLEELCQMVREGQDFLVYDAKTGDDITRSVLTQIILEEDAKGRNLMPIGFLR
                     QLIGFYDDSLQSVVPRYLEVSMENFARHQEQMRGYMEETVGRFFPLNPLDVVNPFEEV
                     ARQNMAIFQKAANLFNPAAEKSGGEPKPAAETARGTKRDDAELDREMRQLKAEMEALQ
                     REMSAMRKAPAGDGD"
     gene            complement(1651..>1914)
                     /locus_tag="P5558_24055"
     CDS             complement(1651..>1914)
                     /locus_tag="P5558_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRY27458.1"
                     /translation="GSNVSYNVKKLVENGYLIQERSSHDRRMTRVRLSEKGLDLTAKI
                     DELYQRNADAIGDGIATGEQLQELNDVLNGLERYWSRIVNFAR"
BASE COUNT          329 a          651 c          663 g          271 t
ORIGIN      
        1 ttcagctggc cgctcgtcgt ggtcgtcgtg gtcctcggtc tcgtcgagga ctgggcgcat
       61 ctgcggcggc gtctggccta atcctccgga gtgtcatcca tgcaggtcat cctcctcgag
      121 cgcgtgccga aactgggcca gatgggccag atcgtgaacg tcaagcccgg ctatgcccgc
      181 aacttcctga ttcccaaggg taaggcgctg cgcgccaccg agacggcgct cggcgccttc
      241 gagagccgcc gtgcccagct cgaggcgcag aacctcgagc gcaggaacga ggccgaggag
      301 gcctcgacca tcatcgacgg cgcctcggtg gtcatcctcc gccaggcgag cgagggcagc
      361 cagctctacg gctcggtcag cccgcgcgac atcgccgacg ccttcaccga gcagggcatc
      421 accttcaccc gccagcagat caggctggag agcccgctca agacgctggg cattcattcg
      481 gtgacggtgg cgctgcaccc cgaggtcgag gtccaggtca gcgtcaacat cgcccgcagc
      541 cacgaggagg ccgagatcca ggccggcaag gcggcgccgg tgctggagga ggaggcgatc
      601 gaccccgaga gcgacgagtt cacccgcggc agcggcttcg acgagctcgg catctgagtg
      661 ctgccggcgg cggaacgggt ggcgtgccgt cgccggtgga gctaagctcg cccggaagtg
      721 gctttcgagg agaccgcaat ggcgccgcgc aaggtcgagg ctgccccgtc gggcggtagg
      781 gaggaaggtg gcggggcgga gggcgagacc cgcaagggcg gcaaggtcgc catcgtcaag
      841 aagtatgcga accggcgcct ctacaacacg gcgtcgagca gctacgtcac cctcgaggag
      901 ctctgccaga tggtgcgcga gggccaggac ttcctggtct acgacgccaa gaccggcgac
      961 gacatcaccc gctcggtgct gacccagatc atcctcgagg aggatgccaa gggccgtaac
     1021 ctgatgccca tcggcttcct gcgccagctc atcggcttct acgacgacag cctgcaatcc
     1081 gtggtgccac gctacctcga ggtctcgatg gagaacttcg cccggcacca ggagcagatg
     1141 cgcggctaca tggaggagac ggtcggccgc ttcttcccgc tcaacccgct cgacgtggtc
     1201 aacccgttcg aggaggtcgc ccgccagaac atggcgatct tccagaaggc ggcgaacctc
     1261 ttcaacccgg ccgccgagaa gtcgggcggc gagccgaagc ccgccgccga gacggcgcgc
     1321 ggcacgaagc gcgacgacgc cgagctcgat cgcgagatgc ggcagctcaa ggccgagatg
     1381 gaggcgctgc agcgcgagat gtcggcgatg cgcaaggcgc ccgccggcga cggcgactga
     1441 cggcgatcac ccgcagctca cgccggctac tcgacccagc cgaacccgtc gtcgaaccag
     1501 ccgccatcgg cgaaggcctc gccgcggccg gccggcggcg cggcatcggc gccgggcagc
     1561 ggccccatgc aggcagcgac cacggcagcg caggcgagcg ccgtgctcgc caggagcgag
     1621 cggcgggaca gaccgcgtgc gtgtgcggcc ctagcgcgcg aagttgacga tccggctcca
     1681 gtagcgctcg agaccattga gcacatcgtt gagctcctgc agctgctcgc ccgtggcgat
     1741 gccgtcgccg atcgcatcgg cgttgcgctg gtagagctcg tcgatcttgg ccgtcaggtc
     1801 cagccccttc tccgaaaggc gtacccgggt catgcgccgg tcgtggctgc tgcgctcctg
     1861 gatcaggtag ccgttctcga ccagcttctt cacgttgtag gagacgttgg agcc
//
LOCUS       DAOTFI010000426         1879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_73006_length_1879_cov_7.326754, whole genome shotgun sequence.
ACCESSION   DAOTFI010000426 DAOTFI010000000
VERSION     DAOTFI010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73006_length_1879_cov_7.326754"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="P5558_24060"
     CDS             <1..1487
                     /locus_tag="P5558_24060"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRY27459.1"
                     /translation="ATKSFPGVKALDGVDFVLRPGEIHALLGENGAGKSTLIKILTGV
                     HLPDSGTVRLFGEPVRFRNARAAIQAGISAVHQERNLIPRMSVAENIMLEDLPERFGF
                     VDYAAVNERALLWIERLGLAIRPDQPVRSLSVAQMQLVEIAKALVLEARILLMDEPTA
                     SITPHETEVLFGLLKQLRDQGAAIVFVSHKLEEVFELCDRVTVLRDGRNACNGLPIGE
                     LDRADVVRFMIGRDERTAKLGDKPVDAADVVLRLDGVATSLGHAGIDLELRRGEILGL
                     YGLVGAGRSELAKALVGIGGVTAGTVTVAGQPAVVPDPTTALQRYRIGYVSEDRKGEG
                     LILIHDVGRNIGITIWRRLSGWLGWLGEGRARAACRPFVEKLAIRTPSMGQKVGNLSG
                     GNQQKVSVAKWLAADCDILIVDEPTVGIDIKTKTDLHELLWELASSGKAILLISSDMP
                     EMVALADRILVMGGFRILGEVRNDHDYGRMSTAIMGHIHAAEAA"
     gene            1505..1834
                     /locus_tag="P5558_24065"
     CDS             1505..1834
                     /locus_tag="P5558_24065"
                     /inference="COORDINATES: protein motif:HMM:NF017174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HRY27460.1"
                     /translation="MQRMGHVIGLKPEHMAEYKRLHAAVWPEVLERIARSNIRNYTIF
                     LREPENLMFGYWEYHGTDFKADMAAMAENARTQEWWKLCGPMQAPLESRAEGEWWARM
                     EEVFHVD"
BASE COUNT          309 a          619 c          676 g          275 t
ORIGIN      
        1 gggcgaccaa gagctttccc ggcgtcaagg cgctggacgg cgtcgacttc gtccttcggc
       61 ccggcgagat ccacgcgctg ctgggcgaga acggcgcagg caagtcgacg ctgatcaaga
      121 tcctgacggg cgtgcatttg cccgacagcg gcacggtccg cctcttcggc gagccggtgc
      181 ggttccggaa tgcccgggcc gccatccagg ccgggatcag cgccgtgcac caggagcgga
      241 acctgatccc gcgcatgagc gtcgccgaga acatcatgct ggaggacctg cccgagcggt
      301 tcggcttcgt cgactatgcg gcggtgaacg agcgcgccct cctctggatc gagcggctcg
      361 gcctcgccat ccggccggac cagccggtgc gctcgctcag cgtggcgcag atgcagctcg
      421 tcgagatcgc caaggcgctg gtgctcgagg cacggatcct gctcatggac gagcccacgg
      481 cctcgatcac gccgcacgag accgaggtgc tcttcggcct gctgaagcag ctccgggacc
      541 agggggcggc catcgttttc gtcagccaca agctcgagga ggtgttcgag ctctgcgacc
      601 gggtgacggt gctgcgcgat ggccggaacg cctgcaacgg cctgccgatc ggcgagctcg
      661 accgggcgga cgtggtgcgc ttcatgatcg gccgcgacga gcgcacggcc aagctcggcg
      721 acaagccggt cgatgcggcc gacgtggtgc tccgcctcga tggcgtggcc accagcctcg
      781 gccatgcggg catcgatctc gagctgcggc ggggcgagat cctgggcctc tacgggctgg
      841 tcggcgccgg gcggagcgag ctcgccaagg cgctggtcgg gatcggcggc gtgaccgccg
      901 gcaccgtgac cgtggcgggc cagccggcgg tggtcccgga cccgacgacg gcgctccagc
      961 gctaccgcat cggctatgtc agcgaggatc gcaagggcga ggggctgatc ctgatccacg
     1021 atgtcggccg caatatcggc atcaccatct ggcgaaggct ttccggctgg ctcggctggc
     1081 tcggcgaggg cagggcccgg gccgcctgcc ggcccttcgt cgagaagctc gccatccgca
     1141 cgccctccat gggccagaag gtcggcaacc tctcgggcgg caaccagcag aaggtctccg
     1201 tcgccaagtg gctggcggcg gattgcgaca tcctgatcgt cgacgagccg acggtcggca
     1261 tcgacatcaa gaccaagacc gacctgcacg agctgctctg ggagctggcc tcctccggca
     1321 aggcgatcct gctgatctcg tcggacatgc cggagatggt ggcgctggcc gaccgcatcc
     1381 tcgtcatggg cggcttccgc atcctgggcg aggtccgaaa cgaccacgac tacggccgga
     1441 tgagcaccgc catcatgggc catatccacg ccgccgaggc ggcctgagcg aagggagagc
     1501 gaccatgcag cggatggggc acgtgatcgg cctgaagccc gagcacatgg cggagtacaa
     1561 gcgcctgcac gccgcggtct ggcccgaggt gctggagcgg atcgccaggt cgaacataag
     1621 aaactacacg atcttcctgc gcgagcccga gaacctcatg ttcggctact gggaatatca
     1681 cggcacggac ttcaaggccg acatggcggc catggccgag aacgccagga cgcaggaatg
     1741 gtggaagctc tgcggcccca tgcaggcccc gctcgagagc cgcgccgagg gcgagtggtg
     1801 ggcgcggatg gaggaggttt tccacgtgga ttgaggggcg ccttgctccc gatagcgggc
     1861 ggtgccggtg ccgaccgtg
//
LOCUS       DAOTFI010000427         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_73076_length_1878_cov_5.728470, whole genome shotgun sequence.
ACCESSION   DAOTFI010000427 DAOTFI010000000
VERSION     DAOTFI010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73076_length_1878_cov_5.728470"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1552)
                     /locus_tag="P5558_24070"
     CDS             complement(80..1552)
                     /locus_tag="P5558_24070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY27461.1"
                     /translation="MLLISADQWRADCLGCEAGLPVRTPAIDAFAGDAVRFRRHFAQA
                     TPCGPSRASLLTGLYAFNHRSIGNGTPLDARHLTLAQIARRHGMRPLLFGYSDTSIDP
                     RSVPAGDPRLLTYEGVAPGFEPACMLLEDGEPWLEHLAERGYGRLTVDEVYATPLGAP
                     ALFAGEDSETAFLTDRFLDFLDRPGSDPWFAHLSYIKPHPPWVAAAPYNSMYERSGLP
                     GPRRAPTIADEAARHPYLTWLLDQKFNGWMGRTFDRPAALDEAVVAELRAVYLGLVSE
                     LDHQLGRVLQALARTGLLERTLVLLTADHGEMLGDHWLLGKNGFWPEAFHVPLIVRHP
                     TRQRPGHVVDAFSEHVDLMPTILGALGLPIPRQCDGHDLSEFLTGQNPANWRRAAVYE
                     HDFRDLEHGRHRQLAGVDDNAACLVARRSADRLYVQFAGLEAGCWGEAAGGGRTPQTD
                     PAVLLDEAQALLRHRLTKAERRLTGCLLTPEGVLGGYDPL"
BASE COUNT          292 a          601 c          704 g          281 t
ORIGIN      
        1 aggcgcgcaa tcccgactgc ccgagctgcg ccatcgccca tctctgccgc tatcgtgcgg
       61 ccgccctgaa agcggcgcct caaagcgggt cgtagccgcc gaggacaccc tcgggcgtga
      121 gcaggcagcc ggtcagtcgc cgctccgctt tggtcaggcg atgccggagc aaggcctgcg
      181 cctcgtccag gagcaccgcc ggatccgtct gcggtgttcg tccgccgccc gcagcctcgc
      241 cccagcagcc ggcctcgaga ccggcgaact gcacgtagag ccggtcggcc gaacgccggg
      301 cgacgaggca ggcggcgttg tcgtcgacac ccgcgagctg gcgatgccgg ccgtgctcga
      361 ggtcgcgaaa atcgtgctcg tagaccgcgg cccggcgcca gttcgccgga ttttgacctg
      421 taaggaattc cgacaggtcg tggccgtcgc actggcgcgg gatcggcagg ccgagggcgc
      481 ccaggatcgt cggcatcagg tcgacatgct cgctgaaggc gtcgacgacg tggccgggtc
      541 gctggcgggt ggggtggcgg acgatgagcg ggacatggaa ggcctccggc cagaagccgt
      601 tcttgccgag cagccagtgg tcgccgagca tctcgccgtg atcggcggtc agcaggacga
      661 gcgtgcgctc caggagcccg gttcgcgcca gcgcctggag gacccggccg agctgatgat
      721 cgagctcgct gacgaggccg agatagaccg cgcggagctc ggccacgacg gcctcgtcga
      781 gcgccgccgg ccggtcgaat gtccgcccca tccagccgtt gaatttctgg tccagcagcc
      841 aggtgaggta ggggtgacgc gcggcctcat cggcgatcgt cggtgcccgc cgtggccccg
      901 gcaggccgct cctctcgtac atcgaattgt aaggtgcagc ggcgacccag ggcgggtgcg
      961 gcttgatgta ggagaggtgg gcgaaccagg gatcgcttcc cggccggtcg aggaagtcca
     1021 aaaaacggtc ggtgaggaag gcggtctcgc tgtcctcgcc ggcgaagagg gcaggtgcgc
     1081 cgagcggcgt cgcgtagacc tcgtcgaccg tgaggcggcc gtagccgcgc tccgccagat
     1141 gctcgagcca gggctcgcca tcttccagca gcatgcaggc aggctcgaag ccgggtgcga
     1201 ccccctcgta ggtcaggagg cgcggatcgc cggcggggac gctgcgcgga tcgatgctgg
     1261 tgtcgctgta gccgaagagc agcggccgca tgccgtgccg gcgggcgatc tgggccaggg
     1321 tgaggtgtcg ggcgtcgagc ggtgtgccgt tgccgatgga gcggtggttg aaggcgtaga
     1381 ggccggtcag cagcgaggcg cgcgacggcc cgcagggcgt cgcctgcgcg aagtgccggc
     1441 ggaagcgcac agcgtcgcct gcgaaggcgt cgatcgccgg ggtgcgcacc ggcaagccgg
     1501 cctcgcagcc gagacagtcg gcccgccact gatcggccga aatcaggagc acgttcaagg
     1561 cgagcacctc gtcgatctct gacgacgcct cgttagtaca cgcatgaaac tcacgccaga
     1621 gcgatcggcg agcaaagcct tcgacggcgg gcggttctgc ttccgttgcg cggccggcgg
     1681 tcgtcgcggc tggatttctt gtcgcgatgc gttaaaaccg aggtacgatt cgagcaacca
     1741 agagggaggt tcggccatgc gtttgcgcac acgaactggc ttgctgacac tcgttctggc
     1801 cggtctggtc agtggtgcgg ctggtgccgc ggagctctcg atcgtcagcg gggcggtcgg
     1861 ccgggacatc gacgagct
//
LOCUS       DAOTFI010000428         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_73369_length_1873_cov_4.962046, whole genome shotgun sequence.
ACCESSION   DAOTFI010000428 DAOTFI010000000
VERSION     DAOTFI010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73369_length_1873_cov_4.962046"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="P5558_24075"
     CDS             complement(<1..1319)
                     /locus_tag="P5558_24075"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRY27462.1"
                     /translation="MRTLAGFTALSLAAGCAFSAPEQAPIAAQPKTLPVRNVTNFTAS
                     LRCMDDLFLKFGKTNYLVTAQEIPDATGKVQTGTKEMLISSISQMSVKSNAFNFVDFE
                     DYGSVFVLGSFLAAPGGPPVPAGFEVPNFYIRGAITQLDSGVIAESASGGIGLEDFDI
                     GASTDQVISVVSVDLNIGNVLNRRILPGVSANNSVVVRRSGSAVDAGATINKLGANFN
                     LTFNQSEGMHQAVRGLIELSAIEVLGKLLQVPYWRCLEIEQTNPEVTAIARGWFNSMS
                     EKERVQFVQRALAAKGRYAGAQNGTMDAATRDAIGAYQAERGLLADGRVNFDLYADLI
                     AGDLALGREPPQDRAPEPLVTASLPVAQPIHLNLTSSRGPAHRFAPGEAMTFTLRSSA
                     DSFAYCYYQDGSGSVARIFPNRFAPDPYLPARETLYLPGNAPFDLVP"
     gene            complement(1418..1768)
                     /locus_tag="P5558_24080"
     CDS             complement(1418..1768)
                     /locus_tag="P5558_24080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27463.1"
                     /translation="MRRMLTAAVLAVALLATTGTVRAEEYNQRPFLGPGEMSKVQRVI
                     SHGRAERFRQEGVADSVGTGPNASIVNTGCGKLSVGNVETTGRPGERLPRENIVIARE
                     IINAPINCGTRGRR"
BASE COUNT          275 a          564 c          730 g          304 t
ORIGIN      
        1 tccggcacga gatcgaaggg cgcgttgccg ggcagataga gcgtctcgcg ggccggcaga
       61 taggggtcgg gcgcgaaccg gttggggaag atgcgcgcca ccgagccact gccgtcctgg
      121 tagtagcaat aggcgaagct gtccgcgctg ctgcgcagcg tgaaggtcat ggcctcgccg
      181 ggcgcgaagc ggtgcgccgg gccgcgcgac gaggtgaggt tgaggtggat cggctgggcc
      241 actggcaggg aggccgtgac cagcggctcc ggggcgcggt cctgcggcgg ctcgcggccg
      301 agcgccaggt cgccggcgat caggtcggca tagaggtcga agttgacgcg gccgtcggcg
      361 agcaggccgc gctcggcctg ataggcgccg atggcgtcgc gggtcgcggc gtccatggtg
      421 ccgttctggg caccggcata gcggcccttg gcggcgaggg cgcgctggac gaactggacg
      481 cgctccttct cggacatgga gttgaaccag ccgcgggcaa tggcggtgac ctcggggttg
      541 gtctgctcga tctccaggca gcgccagtag ggcacctgga gcagcttgcc caggacctcg
      601 atggcgctga gctcgatcag gccgcgcacc gcctggtgca tgccctcgga ctggttgaag
      661 gtgagattga agttggcgcc gagcttgttg atcgtcgcac cggcgtcgac ggcgctgccg
      721 gagcggcgga cgaccacgga gttgttggcg gagacacctg gcaggatgcg ccggttgagc
      781 acgttgccga tattgaggtc gaccgagacg accgagatca cctggtcggt gctcgcaccg
      841 atgtcgaagt cctcgaggcc gatgccgccg gaagcgctct cggcgatcac gccgctgtcg
      901 agctgggtga tggcaccccg gatgtagaag ttgggcacct cgaagccggc cggcacgggc
      961 ggcccgccag gtgcagcgag gaagctgccg agcacgaaga ccgagccgta gtcctcgaaa
     1021 tccacgaagt tgaaggcatt gctcttcacc gacatctgcg agatcgacga gatcagcatt
     1081 tccttggtgc cggtctggac cttgccggtg gcgtccggga tctcctgcgc ggtgaccagg
     1141 tagttggtct tgccgaactt caggaagaga tcgtccatgc agcgcagcga ggcggtgaag
     1201 ttggtgacgt tgcgcaccgg cagcgtcttc ggctgggccg cgatcggcgc ctgctcgggc
     1261 gccgagaagg cgcagccggc cgccagcgaa agcgcggtga agccggcaag ggtgcgcatg
     1321 gctcgcaggt cgggtcttcg catggatcgg gtcatggtct gggccgtccg ttcttctggt
     1381 tgacgggtct ggcgggcggc tcgttggcgg gggcgcctca gcgccggccg cgggtgccgc
     1441 agttgatcgg ggcgttgatg atctcgcggg cgatgacgat gttctcgcgc ggcagccgct
     1501 cgccgggccg gccggtggtc tcgacattgc cgaccgagag cttgccgcag ccggtgttga
     1561 cgatcgaggc attggggccg gtgccgacgg aatcggccac accttcctgg cgaaaacgct
     1621 cggcgcggcc gtggctgatc acccgctgga ccttcgacat ctcgccgggg ccgaggaacg
     1681 ggcgctggtt gtactcttcg gcgcggaccg tcccggtggt cgccagcagg gcgacggcga
     1741 ggacggcggc ggtgagcatg cgacgcatgg ctggtctcct cgtcgccggc cggcccgggc
     1801 gtgggccggc cggctcgggc tagagggtcg aagggtcagt cgcagacgtc ggacttgccg
     1861 atcgagccga tct
//
LOCUS       DAOTFI010000429         1855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_74319_length_1855_cov_9.549444, whole genome shotgun sequence.
ACCESSION   DAOTFI010000429 DAOTFI010000000
VERSION     DAOTFI010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74319_length_1855_cov_9.549444"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="P5558_24085"
     CDS             <1..853
                     /locus_tag="P5558_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRY27464.1"
                     /translation="TAFMGYVLPWGQMSFWGAKVITNLFSAVPIFGETIVTWLWGGFT
                     VDNPTLNRFFALHYLLPFAIFAVVFLHILALHQFGSNNPVGIDITKKDKIPFHPYYTI
                     KDAFGLCVFFLVYAAFVFFAPNVLGHADNYIPANPLVTPPHIVPEWYFLPFYAILRAI
                     TFDIFWIIPAKLGGVIAMFGSIAVLFFLPWLDTSRVRSARFRPIYKWLFWVFLLDCVI
                     LGYVGASPPEGWLPTIGLLATIYYFAHFLIILPLLGKLERPLPLPDSINKPVLPSSGA
                     ASAQPAE"
     gene            857..1621
                     /locus_tag="P5558_24090"
     CDS             857..1621
                     /locus_tag="P5558_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HRY27465.1"
                     /translation="MRRMLEKSLGGLAVALAIVTGASAAEAPEPLSRDWPQQGVFGTF
                     DRHAVQRGFQVFSEVCAGCHGVKYLAFRNLEALGYDEAEIAAIAAQYTVMDGPDEYGD
                     MFERPAVASDRKPVPFANERQARAANGGALPPDLSLITKARAGGEDYLYSLLVGYEEP
                     PAGVELPPGMNYNAYFPGHQIAMAQPLYDDMVEYQDGTVPTISQMAADVTQFLAWVAE
                     PKMEERKQTGIMVMLFLLILAIIFYAYKRRIWADVH"
     gene            complement(1667..>1855)
                     /locus_tag="P5558_24095"
     CDS             complement(1667..>1855)
                     /locus_tag="P5558_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Usg family protein"
                     /protein_id="HRY27466.1"
                     /translation="PGILQSFIWQHYDIAPKYPELHKFLDFWVGNIEGRLHTVNVGRK
                     KLIGPARCGYAQVEYRLH"
BASE COUNT          289 a          653 c          568 g          345 t
ORIGIN      
        1 gaccgccttc atgggctacg tgctgccctg ggggcagatg agcttctggg gggcgaaggt
       61 catcaccaac ctcttctccg cggtgccgat cttcggcgag accatcgtca cctggctctg
      121 gggcggcttc acggtcgaca acccgaccct caaccggttc ttcgccctgc actatttgct
      181 gccgttcgcg atcttcgccg tggtcttcct gcacatcctg gcgctgcacc agttcggctc
      241 gaacaacccg gtcggcatcg acatcaccaa gaaggacaag atcccgttcc atccctacta
      301 caccatcaag gatgcgttcg ggctctgcgt cttcttcttg gtctacgccg ccttcgtctt
      361 cttcgcgccg aacgtgctcg gccacgccga caactacatc cccgccaacc ccttggtgac
      421 gccgccgcat atcgtgccgg aatggtactt cctgccgttc tacgcgatcc tgcgcgccat
      481 caccttcgac atcttctgga tcatcccggc gaagctcggc ggcgtgatcg cgatgttcgg
      541 ctcgatcgcg gtgctcttct tcctgccctg gctcgacacc tcgagggtca ggagcgcccg
      601 gttccggccg atctacaaat ggctcttctg ggtcttcctg ctcgactgcg tcatcctcgg
      661 ctatgtcggc gccagcccgc cggaaggctg gctgccgacg atcgggttgc tcgccacgat
      721 ctactacttc gcccatttcc tgatcatcct gccgctgctc ggcaagctcg agcgaccgct
      781 gccgctgccg gacagcatca acaagccggt gctgccgtcg tccggtgccg catcagctca
      841 gccggcggag tagacgatgc gacgcatgct cgaaaaatcc ctgggcggtc tcgccgttgc
      901 cctggccatc gtgaccggtg ccagcgccgc cgaggcgccc gagcccctct cccgcgactg
      961 gccgcagcag ggtgtgttcg gcaccttcga ccggcacgcc gtgcagcgcg gcttccaggt
     1021 gttcagcgag gtctgcgccg gctgccacgg cgtgaagtac ctggcgttcc gcaacctcga
     1081 ggcgctcggc tacgacgagg ccgagatcgc ggcgatcgcc gcccagtaca ccgtcatgga
     1141 cggtcccgac gaatacggcg acatgttcga gcggccggcg gtggcctcgg acaggaagcc
     1201 cgtgccgttc gccaacgagc ggcaggcacg ggccgccaat ggcggtgccc tgccgcccga
     1261 cctctcgctg atcaccaagg cgcgggccgg cggcgaggac tacctctaca gcctgctcgt
     1321 cggctacgag gagccgccgg ccggcgtcga gctgccgccc ggcatgaact acaacgccta
     1381 tttcccgggc caccagatcg cgatggccca gccgctctac gacgacatgg tggagtacca
     1441 ggacgggacc gtgccgacga tctcgcagat ggcggccgac gtcacccagt tcctcgcctg
     1501 ggtggccgag ccgaagatgg aggaacgcaa gcagaccggc atcatggtga tgctgttcct
     1561 gctgatcctc gccatcatct tctacgccta caagcggcgc atctgggccg acgtccacta
     1621 gttggctgcc ggccgcctgc ccgggcaggc ggcctcgagg cccggctcag tgcaggcggt
     1681 attccacctg cgcgtagccg cagcgggccg gaccgatgag cttcttgcgg ccgacattca
     1741 cggtgtgcag ccggccctcg atgttgccga cccagaaatc gaggaacttg tgcagctccg
     1801 ggtatttcgg ggcgatgtcg taatgctgcc agatgaagga ctggaggatg ccggg
//
LOCUS       DAOTFI010000430         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_74624_length_1850_cov_5.977716, whole genome shotgun sequence.
ACCESSION   DAOTFI010000430 DAOTFI010000000
VERSION     DAOTFI010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74624_length_1850_cov_5.977716"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="P5558_24100"
     CDS             <1..761
                     /locus_tag="P5558_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRY27467.1"
                     /translation="EAVLEDATETLSGLSRYAGLVIAPTTRRPYKHIEFVPLSPGRAL
                     VVTVSDGGMVENRVIEVPIGMSPATLVEAGNYLTARLAGRTFAEARAEIQAEIEKRQG
                     ELDELTQKVVASGLATWGGDDATDGGTLLVRGHANLLDDVSTLVDLERIRHLFTALET
                     KRSFVKLIELSDAGKGVQIFIGAENELFAHSGCSAIIAPFRAGDTAGKSPSVVGAIGV
                     IGPTRMNYSRIIPMVDHTARVISRLLGSPNSEKD"
     gene            761..1396
                     /gene="grpE"
                     /locus_tag="P5558_24105"
     CDS             761..1396
                     /gene="grpE"
                     /locus_tag="P5558_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRY27468.1"
                     /translation="MQQDVDKPETEASVEAAAEAPDGTGAATGQESDATAEIADLKDK
                     LLRALADTENLRRRHTRELEEARRYAMTGFARDLLDVADNMRRAIAAVPANAREENEL
                     VANLLEGVELTERSLLTAFDKHKVARIDPARGDKFDHNRHQAMFEVPSADLPPGHVAE
                     VMQTGWVLADRLLRPAMVGVSKAMPQTATATANDSVGDDAPGRGSTVDTRA"
     gene            1445..>1850
                     /locus_tag="P5558_24110"
     CDS             1445..>1850
                     /locus_tag="P5558_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRY27469.1"
                     /translation="MTGNPKKVVLAYSGGLDTSVILRWLQDTYRCEVVTFTADLGQGE
                     ELEPARAKAEMMGVKEIHIEDLQEEFVRDFVFPMFRANTVYEGTYLLGTSIARPLIAK
                     RLIEIAREVGADAVAHGATGKGNDQVRFELGCY"
BASE COUNT          324 a          640 c          615 g          271 t
ORIGIN      
        1 tcgaggcggt gctcgaggac gccaccgaga cgctctccgg gctctcgcgc tacgccgggc
       61 tcgtcatcgc acccacgacc aggcgaccct acaagcacat cgagttcgtg ccgctcagcc
      121 ccggccgggc actggtggtc acggtatccg atggtggcat ggtcgagaac cgggtgatcg
      181 aggtgcccat cggcatgtcg ccggcgaccc tggtcgaggc gggcaactac ctgacggcgc
      241 gactcgccgg gcgcactttc gccgaggcca gggcggaaat ccaggcggag atcgagaagc
      301 ggcagggcga gctcgacgag ctgacgcaga aggtcgtcgc ctcgggcctc gcgacctggg
      361 gcggcgacga tgcgaccgat ggcggcacgc tccttgtgcg cggacatgcc aacctgctcg
      421 acgatgtcag cacgctggtc gacctcgagc ggatccgcca cctgttcacc gcgctcgaga
      481 ccaagcgcag cttcgtcaag ttgatcgagc tctccgacgc gggcaagggc gtgcagatct
      541 tcatcggcgc cgagaacgag ctgttcgcgc acagcggctg ctcggccatc atcgccccct
      601 tccgggctgg tgacaccgcc ggcaaatcgc catctgtggt cggggcgatc ggcgtgatcg
      661 gcccgacccg catgaactat tcgaggatca tcccgatggt cgaccatacc gcccgggtga
      721 tcagccgcct cctgggcagc cccaacagcg agaaggactg atgcaacagg acgtcgacaa
      781 gccggaaacc gaggccagcg tcgaagctgc ggccgaggcg cccgacggga ccggtgccgc
      841 caccgggcag gaaagcgatg ccacagccga gatcgccgat ctcaaggaca agctcctgcg
      901 cgccctggcc gataccgaga accttcggcg ccggcacacg cgcgagctgg aggaggcgcg
      961 ccgctatgcg atgaccggct tcgctcgcga tctcctggac gtggccgaca acatgcgccg
     1021 ggcgatcgcc gccgtgccgg ccaatgcccg tgaggagaac gagctcgtgg ccaacctgct
     1081 cgaaggcgtc gagctgaccg agcgctcgct gctcacagcc ttcgacaagc acaaggtggc
     1141 ccgcatcgac ccggcgcgcg gcgacaagtt cgatcacaac cgccatcagg cgatgttcga
     1201 ggtgccgtcc gccgatctgc cgcccggcca tgtcgccgag gtgatgcaga ccggctgggt
     1261 cttggccgat cggctcttgc gcccggccat ggtcggcgtc agcaaggcga tgccccaaac
     1321 ggcgaccgca acggcgaacg attcggtcgg tgacgatgcg cctgggcgcg gctcgaccgt
     1381 cgatacgcgc gcctgacatc cgccctcttt cgagcctttt cgccccccca atcgaggaac
     1441 ggccatgacc ggcaatccca agaaggtggt gctcgcctac tccggcggcc tcgacacctc
     1501 ggtcatcctg cgctggctgc aggacaccta tcgctgcgag gtcgtcacct tcaccgccga
     1561 tctcggccag ggcgaggagc tggagccggc ccgcgccaag gccgagatga tgggggtgaa
     1621 ggagatccat atcgaggatc tccaggagga gttcgtccgc gacttcgtct tcccgatgtt
     1681 ccgcgccaac accgtctacg agggaaccta tctcctcggc acctcgatcg cccggccgct
     1741 gatcgccaag cggctgatcg agatcgcgcg cgaggtcggt gccgacgccg tggcgcacgg
     1801 ggcgacgggc aagggcaacg accaggtccg cttcgagctc ggctgctatg
//
LOCUS       DAOTFI010000431         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_74735_length_1848_cov_5.769102, whole genome shotgun sequence.
ACCESSION   DAOTFI010000431 DAOTFI010000000
VERSION     DAOTFI010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74735_length_1848_cov_5.769102"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="P5558_24115"
     CDS             <1..892
                     /locus_tag="P5558_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY27470.1"
                     /translation="TDDGSQTFCVPMASVIHGFMYNKDAFAELGLEVPTTEEEFFAVL
                     DAIKADGSYEPLAMGTADQWEAATMGYTNIGPNYWQGEEGRKALIAGEAKLTDEAYVA
                     PFRTIARWADYLPSGFEAQSYPDSQNLFTLGRAAIYPTGSWEISPFQRDAFFEMGAFK
                     PPVPNAGDPCFISDHVDIGMGINKASPNQEAARTFVEWVGSAEFAELYSNALPGFFSL
                     SSHPITLEDPLAQEFIDWRNECSSSIRLAHQILSRGTPNLWNDMWVTSANVVNGVVTP
                     EEAGAHLQEGLASWYAPQQN"
     gene            945..>1848
                     /locus_tag="P5558_24120"
     CDS             945..>1848
                     /locus_tag="P5558_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY27471.1"
                     /translation="MQHARAAARRKPFPWHVVVFLAPAVLIYTAVMIYPLAETLRLSF
                     FDRTDEGARFWVGLVNFERLFLDPHWADQFWNALANNFYFFVVHMAVQNPIGVLLAAL
                     LSMTHLPGRTFYRTAIFVPAMLSVVIIGFAWKLILSPIWGVSETLLGLVGLDFLFGPW
                     LGKEGSALTTLALISVWQYVGIPMMLIYAALLAIPEDISEAADLDGVRGMALFFKVKL
                     PLIWPTIGIISILTFIGNFNAFDLIYAVQGALAGPNFSTDILGTFLYRTFFGHQLELG
                     DPHMGATIATAMFLIILVGVMAYLW"
BASE COUNT          281 a          693 c          543 g          331 t
ORIGIN      
        1 cacggatgac ggcagccaga ccttctgcgt gccgatggcc tcggtgatcc acggcttcat
       61 gtacaacaag gacgccttcg cggagctcgg cctcgaggtg ccgacgacgg aggaggagtt
      121 cttcgccgtg ctggatgcca tcaaggccga cggctcctac gagccgctcg ccatgggcac
      181 ggccgaccag tgggaagcgg cgaccatggg ctacaccaat atcggcccca actactggca
      241 gggcgaggag ggccgcaagg cgctgatcgc cggcgaggcc aagctcaccg acgaggcgta
      301 tgtggcgccg ttccggacga tcgcccgctg ggccgactat ctgccctccg gcttcgaggc
      361 gcagtcctac ccggacagcc agaacctctt caccctcggc cgggcggcga tctatcccac
      421 cggctcctgg gagatctcgc ccttccagcg cgacgccttc ttcgagatgg gcgccttcaa
      481 gccgccggtc ccgaacgcgg gcgacccctg cttcatcagc gaccatgtcg acatcggcat
      541 gggcatcaac aaggcgagcc cgaaccagga ggctgcgcgg accttcgtcg agtgggtggg
      601 ctccgccgag ttcgccgagc tctactcgaa cgcgctgccg ggcttcttct cgctctccag
      661 ccacccgatc acgctcgagg acccgctcgc ccaggagttc atcgactggc ggaacgagtg
      721 cagctcctcg atccgcctcg cccaccagat cctcagccgc ggcacgccca atctctggaa
      781 cgacatgtgg gtgacctcgg ccaacgtcgt caacggcgtg gtgacgcccg aggaggccgg
      841 tgcccacctg caggagggcc tcgcctcctg gtacgcaccg cagcagaatt gacacgaggc
      901 ggccggctcc ggccggccgc ccccctcccg tcgaggcccg acccatgcag cacgcccgag
      961 ccgccgcccg ccgcaagccc tttccctggc acgtggtcgt cttcctcgca cctgccgtcc
     1021 tcatctacac cgcggtgatg atctacccgc tggcggagac cctgcggctt tccttcttcg
     1081 accgcacgga cgagggggcg cgcttctggg tcgggctcgt caatttcgag cgcctcttcc
     1141 tcgacccgca ctgggccgac cagttctgga acgcgctcgc caacaacttc tacttcttcg
     1201 tcgtgcacat ggccgtgcag aacccgatcg gcgtgctgct cgcggccctc ctgtcgatga
     1261 cccatctgcc cggccgcacc ttctaccgca ccgccatctt cgtcccggcg atgctctcgg
     1321 tcgtgatcat cggcttcgcc tggaagctca tcctgagccc catctggggg gtctccgaga
     1381 cgctgctcgg cctcgtcggg ctggacttcc tcttcggccc ctggctcggc aaggagggct
     1441 cggcgctgac caccctcgcc ctgatctcgg tctggcaata tgtcggcatc ccgatgatgc
     1501 tgatctatgc ggccctcctc gccatcccgg aggacatcag cgaggccgcc gatctcgacg
     1561 gcgtgcgcgg catggccctc ttcttcaagg tcaagctgcc gctgatctgg ccgaccatcg
     1621 ggatcatctc gatcctcacc ttcatcggca acttcaacgc cttcgacctg atctacgcgg
     1681 tccagggggc cctcgccggg ccgaacttct cgaccgacat cctcggcacc ttcctctacc
     1741 gcaccttctt cggccaccag ctcgagctcg gtgacccgca catgggggcg accatcgcga
     1801 ccgccatgtt cctcatcatc ctggtcggcg tgatggccta tctctggg
//
LOCUS       DAOTFI010000432         1841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_75078_length_1841_cov_7.014558, whole genome shotgun sequence.
ACCESSION   DAOTFI010000432 DAOTFI010000000
VERSION     DAOTFI010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75078_length_1841_cov_7.014558"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="P5558_24125"
     CDS             <1..832
                     /locus_tag="P5558_24125"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY27472.1"
                     /translation="PADRIAVFDNDGTLWGEQPVYFQFLFAVDRLKEMAAADPSVLTS
                     DVLRAAAAGDMEKIAAAGTDGLLEIVSTTHSGMSVEAFQADVRDWLDTATHPETGMAY
                     DAMIYQPMLELLSYLRDQGFTTWIVSGGGLHFIRVFSEDAYNIPPPQVVGTTGNATYD
                     VIDGVPTVLKDPGIFFLDDKAGKPVAIDARIGKRPILAAGNSDGDFEMLEWTTAGEGP
                     RLGLLVHHTDAEREWAYDRESHVGRLDRGLDEAEARGWLLVDMAEDWARVWPAGAAAR
                     "
     gene            843..1730
                     /locus_tag="P5558_24130"
     CDS             843..1730
                     /locus_tag="P5558_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRY27473.1"
                     /translation="MKQKDLARRHRVTDGRKFRLEDVDPADTAGLDIEKDDARELLAD
                     SIKDLRKLQERLYAEGRWAVLVVLQAMDAGGKDSAIEHVMTGINPQGCDVRSFKQPGP
                     IELRHDFLWRHVAGLPQRGKIVIFNRSHYEEVLVVRVHPEILEKQSLPPQLVGDDLWE
                     NRFKDIRAFERHLANSGTVVLKFFLHISKDEQARRFLDRIDEADKNWKFSFGDLEERK
                     HWDDYMAACDDMIRSTARDHAPWHVVPANNKWYARMVISSVLTETLERLDPQYPTVTD
                     EERQRMKAARDVLAHEAGL"
BASE COUNT          332 a          625 c          621 g          263 t
ORIGIN      
        1 cccggccgac cgcatcgccg tcttcgacaa tgacggcacg ctctggggcg agcagccggt
       61 ctacttccag ttcctcttcg ccgtcgatcg cctgaaggag atggcggccg ccgacccttc
      121 cgtcctgacc tcggacgtgc tccgcgccgc ggccgcgggc gacatggaaa agatcgcggc
      181 cgccggcacg gacggcctgc tcgagatcgt ctcgaccacg cattccggca tgtcggtcga
      241 ggccttccag gccgatgtcc gggactggct cgacaccgct acccatcccg agacgggcat
      301 ggcctacgac gccatgatct accagccgat gctggagctc ctgagctacc tgcgcgacca
      361 gggcttcacc acctggatcg tctccggcgg cggcctgcac ttcattcgcg tcttctcgga
      421 ggacgcctac aacattcccc cgccccaggt cgtgggcacg accggcaacg cgacatacga
      481 cgtgatcgac ggcgtgccca cggtgctcaa ggacccgggc atcttcttcc tcgacgacaa
      541 ggccggcaag cccgtggcga tcgacgccag gatcggcaag cggccgatcc tcgccgccgg
      601 caactcggac ggcgacttcg agatgctgga atggacgacg gcaggcgagg ggccgcgcct
      661 cgggctgctc gtgcaccaca ccgacgccga gcgcgaatgg gcctacgacc gcgagagcca
      721 cgtcggcagg ctcgaccgcg ggctcgacga ggcggaggca cgcggctggc tgctcgtcga
      781 catggcggag gactgggcac gggtgtggcc ggccggtgcg gcggcgcggt gacggagcag
      841 gcatgaagca gaaggacctc gccaggcgcc atcgggtcac cgatggcagg aagttccggc
      901 tcgaggacgt cgatccggcc gacactgccg gcctcgacat cgagaaggac gatgcccggg
      961 agctcctcgc cgacagcatc aaggacctgc gcaagctgca ggagcggctc tacgccgagg
     1021 gcagatgggc ggtcctggtg gtgctgcagg ccatggacgc cggcggcaag gacagcgcca
     1081 tcgagcacgt catgaccggc atcaacccgc aaggctgcga tgtccgctcg ttcaagcagc
     1141 cggggccgat cgagctccgg cacgacttcc tctggcgcca tgtcgcgggc ctgccgcagc
     1201 ggggcaagat cgtcatcttc aatcgctccc actacgagga ggtgctcgtc gtccgcgtgc
     1261 accccgagat cctcgaaaag cagagcctgc cgccgcagct cgtgggcgac gacctctggg
     1321 agaatcgctt caaggacatc cgggcgttcg agcgccacct ggccaacagc ggcaccgtgg
     1381 tcctgaagtt cttcctccac atctcgaagg acgagcaggc ccggcgtttc ctcgaccgca
     1441 tcgacgaggc ggacaagaac tggaagttct ccttcggcga tctggaggag cgcaagcact
     1501 gggacgacta catggcggcg tgcgacgaca tgatccgcag caccgcgcgg gaccatgcgc
     1561 cctggcatgt cgtgccggcc aacaacaagt ggtacgcgcg aatggtgatc tcctcggtgc
     1621 tcacggagac cctcgagcga ctcgatcccc aatacccgac ggtgaccgac gaggagaggc
     1681 agcgaatgaa agcggcgcgc gacgttctgg cacatgaggc aggtttgtga tggacggcgc
     1741 ccacgacacg accggcgtct cggctcggga acaggtcgag gcgctccgcg agcgcatggg
     1801 cgaggcgatc atcggccagc gcgaggtgat cgagcgcctg c
//
LOCUS       DAOTFI010000433         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_77478_length_1802_cov_6.544934, whole genome shotgun sequence.
ACCESSION   DAOTFI010000433 DAOTFI010000000
VERSION     DAOTFI010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77478_length_1802_cov_6.544934"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="P5558_24135"
     CDS             complement(<1..746)
                     /locus_tag="P5558_24135"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRY27474.1"
                     /translation="MSDPKGLWQASFELDLEPPPLLLEALEEMALSASIFTAAVDEAD
                     EATRWRVDLLFAEEPDATTLGAEIGALLAAHDLPAGVDVAIAWLAHQDWLEAVRMTRP
                     PMTVGRFLVHGPDDAPPANARAVPLLIEAGLAFGSGEHATTTACLLAFDRELRRRPFA
                     RVLDMGCGSAILGIAAARVNRHTRVLAVDNDPLAVAVARDNAKKNGVAGRVACLVSEG
                     YGDARIRRSGPFDLIFANILADPLVAMAGDL"
     gene            complement(760..>1802)
                     /locus_tag="P5558_24140"
     CDS             complement(760..>1802)
                     /locus_tag="P5558_24140"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY27475.1"
                     /translation="IRDAIAYLRLTAQPADDLAFERIVNLPRRGIGDAAIQALRGLSR
                     RVEIPLLQAARQMVETDELATRTRNALMAFLGLVDGWRTTLAEAPPRELAEQILDESG
                     YTAMWQNDRSPDAPGRLDNLKELVKAIEEFPTLAAFLEHVSLVMDAVGESGSEQVSVM
                     TLHGAKGLEFNSVFLAGWEDGLFPNQRALDEGGGKALEEERRLAYVGLTRARHRCTVS
                     FAANRRIYNQWSSSVPSRFIEELPAEHVEHLHRNPAMRPDAESFMDDLVVPQVARFRS
                     RRAELGKVIEGRAEILTTPPGAGASFSVGDRIFHQKFGYGRVAAVDGNKLEIAFEKSG
                     VKKVISSFVEAA"
BASE COUNT          273 a          633 c          629 g          267 t
ORIGIN      
        1 agatcgcccg ccatggccac cagcggatcg gccaggatat tggcgaagat caggtcgaac
       61 ggcccggacc ggcggatgcg cgcatcgcca tagccttcgc tcacgaggca agcgacccgg
      121 ccggccacgc cgttcttctt ggcattgtcg cgggccacgg cgacggcgag cgggtcgttg
      181 tcgaccgcga ggacccgggt gtgacggttc acccgtgccg cggcgatgcc gagaatggcc
      241 gagccgcagc ccatgtcgag cacccgggcg aagggtcggc ggcgcagctc gcgatcgaac
      301 gccaggaggc aggccgtggt ggtggcatgc tcgcccgagc cgaaggcgag cccggcctcg
      361 atcaagagcg gcaccgcacg ggcgttcgcc ggcggcgcat cgtcgggccc gtgcacgagg
      421 aagcggccga cggtcatggg cggccgggtc atgcgcaccg cctcgagcca gtcctggtgc
      481 gcgagccagg cgatggcgac atccaccccc gccggcaggt cgtgcgccgc gagcagcgca
      541 ccgatctcgg cacccagggt agtcgcgtcc ggctcctcgg cgaacagcag atcgacccgc
      601 cagcgcgtcg cctcgtccgc ctcgtcgacg gcggcggtga agatcgaggc cgagagcgcc
      661 atctcctcga gcgcctcgag cagcaagggc ggcggctcga ggtcgagctc gaagctcgcc
      721 tgccagagtc cctttggatc gctcacgaac gtcgccgcat caggccgcct cgacgaagct
      781 cgagatcacc ttcttcaccc cggacttctc gaaggcgatc tccagcttgt tgccgtcgac
      841 ggcggcgacc cggccatagc cgaacttctg gtggaagatc cggtcgccga cgctgaaact
      901 ggcgccggcg ccgggcggcg tggtcaggat ctcggcacgc ccctcgatga ccttgccgag
      961 ctcggcgcgc cggctgcgga accgggccac ctgcggcacc acgagatcgt ccatgaagct
     1021 ctcggcatcg ggtcgcatgg cggggttgcg atgcaggtgc tcgacatgct cggccggcag
     1081 ttcctcgatg aaccgcgagg gcacgctcga tgaccactgg ttgtagatcc ggcgattggc
     1141 ggcgaagctg acggtgcaac ggtgccgggc acgggtcagc ccgacatagg cgagccgccg
     1201 ctcctcctcc agcgccttgc cgcccccttc gtcgagggcg cgctggttcg ggaagagccc
     1261 gtcctcccag cctgccagga agacgctgtt gaactcgagc cccttggccc cgtgcagcgt
     1321 catcaccgaa acttgctcgc tgccgctctc gccgaccgca tccatgacca ggctgacatg
     1381 ctcgaggaac gccgcgagcg tcggaaactc ctcgatcgcc ttgaccagct ccttgaggtt
     1441 gtcgagccgg ccgggcgcgt cgggcgagcg gtcgttctgc cacatcgccg tgtagccgct
     1501 ctcgtccagg atctgctccg ccaattcgcg cggcggcgcc tcggcgagcg ttgtccgcca
     1561 gccgtcgacg aggccgagaa aggccatgag cgcgttcctc gtgcgggtcg ccagctcgtc
     1621 ggtctcgacc atctggcggg ctgcctggag cagcgggatc tcgacgcgcc gggacagccc
     1681 gcgcaacgcc tgtatcgccg cgtcgccgat gccgcggcgg ggcaggttga cgatccgctc
     1741 gaaggccagg tcgtcggccg gctgggcggt cagcctgaga taggcgatgg catcgcggat
     1801 ct
//
LOCUS       DAOTFI010000434         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_79859_length_1762_cov_5.884593, whole genome shotgun sequence.
ACCESSION   DAOTFI010000434 DAOTFI010000000
VERSION     DAOTFI010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79859_length_1762_cov_5.884593"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..300)
                     /locus_tag="P5558_24145"
     CDS             complement(19..300)
                     /locus_tag="P5558_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRY27476.1"
                     /translation="MKTPSHPGPVVRHEVLEPLGLSVTDAAEVLGVARPTLSNLLNGK
                     AALTPEMAIRIEKAFGPKADHLLRVQLAWDLAEVRRRQDQIVVRRFEPA"
     gene            complement(393..833)
                     /locus_tag="P5558_24150"
     CDS             complement(393..833)
                     /locus_tag="P5558_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY27477.1"
                     /translation="MPLIVPIATEHRARWQELYRGYATFYERELTDAHAETVWGWLQD
                     PAHELEGRLALVDGRPVGLAHFRRMPSPLRGADIGFLDDLFVDPKARAEGVGRALIEA
                     VAGIARERGWSVVRWLTADDNYRARALYDRLAVKTAWNLYELAP"
     gene            complement(805..1605)
                     /locus_tag="P5558_24155"
     CDS             complement(805..1605)
                     /locus_tag="P5558_24155"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRY27478.1"
                     /translation="MERKFWLGTGWKMNKTRAEARAYAEALRAAPGLNELPLRLFVLP
                     PFTALAEVAAALAGSGVMVGAQNCHWASAGAWTGEISAPQIAECGAALVELGHSERRA
                     HFGETDVAINAKVKAVLAAGLRPLVCVGETAEERALDAATVTVARQARMALAGVAAAD
                     LDRVLLAYEPVWAIGEGGTPASPDYADAIHGALKNAVMDLHGRSVPVLYGGSVNAANA
                     LALASQPEIDGLFVGRAAWTVEGLLGLARAITPIVEAKPHAPDRADRN"
BASE COUNT          259 a          620 c          628 g          255 t
ORIGIN      
        1 gctctgacgg ctccccgttc aggcgggctc gaagcgccgc acgacgatct ggtcctgccg
       61 ccgcctgacc tcggcgaggt cccaggcaag ttgcacacgc agcaggtgat ccgctttcgg
      121 gccgaaggcc ttctcgatgc gaatggccat ctcgggcgtc aaagcggcct tgccgttgag
      181 caggttcgac agggtcggcc gggccacgcc gagcacctcg gcggcgtcgg tgacgctcag
      241 cccgagcggc tccagcactt cgtggcggac gaccggtccg ggatgggatg gggtcttcat
      301 tcgcgtctcg ctgagctggc gcaggcccgc gtgccggtgg cgctgcagcc tgtaacgcta
      361 cgcctgtcaa tcagcgccgt ctgccggcaa gatcagggag cgagctcgta caaattccac
      421 gccgtcttga ccgccagccg gtcgtagagc gcccgggcgc ggtagttgtc gtcggcagtg
      481 agccagcgca ccacgctcca gccgcgctcg cgggcgatcc cggcgaccgc ctcgatcagg
      541 gcccggccga cgccctcggc ccgcgccttc gggtcgacga agagatcgtc gagaaagccg
      601 atatccgcac cccggagcgg gctcggcatg cggcggaaat gggcgaggcc gaccggccgg
      661 ccgtcgacga gggcgaggcg gccctccagc tcgtgggcgg gatcctgcag ccatccccag
      721 acggtctcgg catgcgcatc ggtgagctcg cgctcgtaga acgtggcgta gccccgatag
      781 agctcctgcc agcgagcccg gtgctcagtt gcgatcggca cgatcagggg catggggctt
      841 cgcctcgacg atgggggtga tcgcccgggc gaggccgagg agcccctcga ccgtccaggc
      901 ggcgcggccg acgaagaggc cgtcgatctc cggctggctg gcgagggcca gggcgttcgc
      961 cgcattgacg ctgccgccat agagcaccgg cacgctgcgg ccgtggaggt ccattaccgc
     1021 gttcttgagg gcgccatgga tggcgtcggc atagtccggc gaggccggcg tgccaccctc
     1081 gccgatcgcc cagaccggct cgtaggcgag gagcacgcga tcgaggtcgg ccgcggcgac
     1141 acccgccagc gccatgcgcg cctggcgggc gacggtcacc gtggcggcat cgagcgcgcg
     1201 ctcctccgcc gtctcgccga cgcagaccag cggccgaagc ccggcggcga gcaccgcctt
     1261 gaccttggcg ttgatggcga catccgtctc gccgaaatgg gcgcgccgct cgctgtggcc
     1321 gagctcgacc agggcggccc cgcactcggc gatctgtggc gccgagatct cgccggtcca
     1381 ggccccggca ctggcccagt ggcagttctg cgcccccacc atgaccccgc tgccggcgag
     1441 ggccgccgcc acttcggcga gcgccgtgaa gggcggcagc acgaagagcc gcagcggcag
     1501 ctcgttcagg cccggtgcgg cgcgcagcgc ctcggcatag gcccgggcct cggcgcgggt
     1561 cttgttcatc ttccagccgg tgccgagcca gaacttccgc tccatccctg gtctccccgt
     1621 tcggcatccc ttgacagcgt gccgcattct tgtgcaagct gacgaaagaa agttcaagga
     1681 atgtataaaa gttcatgacg ggggagacgg ccaggggaca ggacgagctg gggctcgcca
     1741 cgcgggcggc ctggctctcc ta
//
LOCUS       DAOTFI010000435         1748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_80767_length_1748_cov_6.897224, whole genome shotgun sequence.
ACCESSION   DAOTFI010000435 DAOTFI010000000
VERSION     DAOTFI010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80767_length_1748_cov_6.897224"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..376)
                     /locus_tag="P5558_24160"
     CDS             complement(5..376)
                     /locus_tag="P5558_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HRY27479.1"
                     /translation="MTPGVELGALPRDADGPVFAEPWQAQAFAMAVLLHEQGLFTWPE
                     WAATLAAEIASAPAIDDPVDGSAYYRHWLAALERLVVARGVASESNLAGLAAAWQTAA
                     AATPHGRPIELPPEVQSAAKQ"
     gene            complement(373..1044)
                     /gene="nthB"
                     /locus_tag="P5558_24165"
     CDS             complement(373..1044)
                     /gene="nthB"
                     /locus_tag="P5558_24165"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HRY27480.1"
                     /translation="MNGGQDLGGMMGFGPVRPEPGEPVFHSQWERRVFALTGAMARPG
                     GWNIDQSRFARESLDPGRYLTSTYYEIWLAGLERLLAERGLVTADELAAGRPLGEPLP
                     VPAILEAAAVEPTLAKGASTSRPADRPPRFAVGAPVRARLLNPEGHTRLPRYVRGRPG
                     VVTADHGFNVLPDINATGRGESPERLYTVRFAARDLWGDDADPTASVSVDAWERYLEP
                     AGDGA"
     gene            complement(1041..1667)
                     /gene="nthA"
                     /locus_tag="P5558_24170"
     CDS             complement(1041..1667)
                     /gene="nthA"
                     /locus_tag="P5558_24170"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HRY27481.1"
                     /translation="MHDHGDHDHADGSHLTPIEARVRALETILTGKGYVDPAALDRLI
                     ETYETRIGPRNGARVVARAWHDPAYRARLLADATSAIAELGFTGRQGEHMVAVPNTAT
                     VHNLVVCTLCSCYPWPVLGLPPVWYKSAPYRSRAVLDPRSVLAAFGLVLPPEKAIRVW
                     DSTAEIRYLVVPERPAGSQGWSAERLATLVTRDSMIGTGLALDPADAP"
BASE COUNT          233 a          591 c          677 g          247 t
ORIGIN      
        1 tcttctactg ctttgccgcc gactgaacct cgggcggcag ctcgatcggc cggccgtgcg
       61 gggtcgctgc ggcggccgtc tgccaggcgg cggccagccc ggcgaggttg ctctcggagg
      121 cgacgcccct ggcgacgacc aagcgctcca gcgctgccag ccagtggcga taataggccg
      181 agccgtcaac gggatcatcg atcgccggag cgctcgcgat ctccgcagcc agcgtcgcgg
      241 cccattccgg ccaggtgaag agaccctgct cgtggagcag cactgccatg gcgaaggcct
      301 gggcctgcca gggctcggcg aagacgggtc catccgcatc gcgcggcagc gctccgagct
      361 cgacgccggg cgtcatgcgc cgtcgccggc cggctcgaga tagcgctccc aggcatcgac
      421 ggagacgctg gcggtcgggt cggcgtcgtc gccccagagg tcgcgggcgg cgaaacgcac
      481 ggtgtagaga cgttccgggc tctcgccccg cccggtcgca ttgatgtccg gcagcacgtt
      541 aaacccgtgg tcggcagtga cgacgccggg tcggccgcgc acgtagcgcg gcaggcgggt
      601 gtggccttcg gggttcagga gccgggcccg cacgggggcg ccgacggcga agcgcggcgg
      661 ccggtcggcg gggcgggagg tcgaggctcc cttcgccagc gtcggttcga cggccgccgc
      721 ctcgaggatg gccgggacgg gcagcggctc gccgagcggc cggccggctg ccagctcgtc
      781 cgcggtcacg aggccacgct cggcgagcag ccgctcgagg ccggcgagcc agatctcgta
      841 gtaggtcgag gtcaggtagc ggccgggatc gaggctttcc cgggcgaagc gcgactggtc
      901 gatgttccag ccgccagggc gcgccatggc gccggtcagg gcgaagacgc ggcgctccca
      961 ttggctgtgg aacacgggct cgcccggctc cgggcgcact ggcccgaagc ccatcatgcc
     1021 gcccaggtcc tggccgccgt tcatggcgcg tccgccgggt cgagggcgag gccggtgccg
     1081 atcatgctgt cgcgggtgac cagcgtggcc agccgctccg cgctccagcc ctgcgatccg
     1141 gcgggtcgtt cgggaaccac cagataacgg atctcggcgg tcgaatccca gacccggatc
     1201 gctttctccg gcggcagcac gaggccgaac gcggccagga ccgagcgcgg gtcgagcacg
     1261 gcgcggctgc ggtagggggc ggatttgtac cagaccggcg gcaggccgag caccggccac
     1321 gggtagcagg agcagagcgt gcagacgacg aggttgtgca cggtggccgt gttcgggacc
     1381 gccaccatgt gctcgccctg gcggccggtg aagccgagct cggcgatggc ggaggtggcg
     1441 tcggccagga ggcgggcgcg ataggccggg tcgtgccacg cccgggccac gaccctggcc
     1501 ccgtttctcg ggccgatccg ggtctcgtag gtctcgatca ggcggtcgag cgcggccggg
     1561 tcgacatagc ccttcccggt caggatggtc tcgagggcgc gcacccgggc ctcgatgggg
     1621 gtgaggtggc tgccatctgc gtggtcatga tcgccatggt cgtgcaagga tgccttactc
     1681 ctcgggcctt ggcctgaacc gccccgggtt tgccggaggc tggttggttt gagtctcacg
     1741 ctgccaga
//
LOCUS       DAOTFI010000436         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_80909_length_1746_cov_7.463631, whole genome shotgun sequence.
ACCESSION   DAOTFI010000436 DAOTFI010000000
VERSION     DAOTFI010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80909_length_1746_cov_7.463631"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /gene="sdhA"
                     /locus_tag="P5558_24175"
     CDS             complement(<1..1131)
                     /gene="sdhA"
                     /locus_tag="P5558_24175"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HRY27482.1"
                     /translation="MTETIPTQPREGALVHGMYRDGEEIADRKAPKAPIDQMWTKRKF
                     DAKLVNPANRRKLSVIIVGTGLAGAAAAATMGEAGYNVKAFYYQDSARRAHSIAAQGG
                     INAAKNYNDDGDSIYRLFYDTVKGGDYRSRETNVYRLAEVSANIIDQCVAQGVPFARE
                     YGGLLDNRSFGGVQVARTFYAAGQTGQQLLIGAYQALERQVAKGSVTQYQRHEMLELI
                     VVDGRARGIVARDMVTGEIQTHLADAVVLASGGYGNVFFLSTNAMGCNVTATWRAHRK
                     GAYFGNPCYTQIHPTCIPVSGDHQSKLTLMSESLRNDGRIWVPKRAEDCDKDPREIAE
                     EDRDYYLERIYPAFGNLVPRDIASRQAKNMCDEGRGVGPMVGD"
     gene            complement(1134..>1746)
                     /locus_tag="P5558_24180"
     CDS             complement(1134..>1746)
                     /locus_tag="P5558_24180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRY27483.1"
                     /translation="MHMYGNLKLFAGERAFNEYAEHLREILVPILPHSGFLWLFRLLL
                     VVALVVHAYAAFTLWSRANGARPTRYAVKSAAKGAARSTMMRWGGVFLLLFLMWHLIH
                     FTIVKPAINADYPAATIQASPYVMAVASFKLWWVVLIYVLALVALGLHLFHGVWSGAQ
                     TLGWTGSPAKARIAKATAHLVAAVVAIGFALPPLAIIAGIVKG"
BASE COUNT          254 a          550 c          651 g          291 t
ORIGIN      
        1 gtcgccgacc atcgggccga cgccgcggcc ctcgtcgcac atgttcttgg cctgccggct
       61 ggcgatgtcc cgggggacga ggttgccgaa ggccgggtag atccgctcca ggtagtagtc
      121 gcggtcctcc tcggcgatct cgcgcgggtc cttgtcgcag tcctcggccc gcttggggac
      181 ccagatccgg ccgtcgttgc gcagcgactc gctcatcagg gtgagctttg actggtggtc
      241 gccggacacc gggatgcagg tcgggtggat ctgggtgtag cacgggttgc cgaagtacgc
      301 acccttgcgg tgcgcccgcc aggtcgcggt gacgttgcag cccatcgcat tggtcgagag
      361 gaagaagacg ttgccgtaac cgccgctggc cagcacgacc gcgtcggcga ggtgggtctg
      421 gatctcgccg gtgaccatgt cgcgggcgac gatgccgcgg gcccggccgt cgacgacgat
      481 gagctcgagc atctcgtgcc gctggtactg ggtgaccgag cccttggcga cctgccgctc
      541 gagggcctgg taggcgccga tgagcagctg ctggcctgtc tggccggccg cgtagaaggt
      601 gcgcgccacc tggacgccac cgaaggagcg gttgtccagc aggccgccgt actcgcgggc
      661 gaaggggacg ccctgggcga cgcactggtc gatgatgttc gcgctcacct cggcgagccg
      721 gtagacgttg gtctcgcgcg agcggtagtc gccgcccttg accgtgtcgt agaacagccg
      781 gtagatcgag tcgccgtcgt cgttgtagtt cttcgcggcg ttgatgccgc cctgggcggc
      841 gatcgagtgg gcccggcggg cgctgtcctg gtagtagaag gccttgacgt tgtagccggc
      901 ctcgcccatc gtcgccgcgg cggccgcgcc ggccagtccg gtgccgacga tgatgaccga
      961 cagcttgcgc cggttggccg ggttgacgag cttggcgtcg aacttgcgct tggtccacat
     1021 ctggtcgatg ggggccttcg gggccttgcg gtcggcgatc tcctcgccgt cgcggtacat
     1081 cccgtgcacg agcgcgccct cgcgcggctg ggtggggatc gtctcggtca tgatcagccc
     1141 ttcacgattc cggcgatgat ggccaggggc ggcagcgcga agccgatggc gacgacggcc
     1201 gcgacgaggt gtgcggtggc cttggcgatg cgggccttgg ccggcgagcc ggtccagccc
     1261 agcgtctgcg cgccgctcca cacgccgtgg aacaggtgca ggccgagagc gacgagcgcg
     1321 agcacgtaga tgagcacgac ccaccacagc ttgaagctcg ccaccgccat gacatagggg
     1381 ctggcctgga tggtggccgc cgggtagtcg gcgttgatgg ccggcttgac gatggtgaag
     1441 tggatgaggt gccacatgag gaagagcagc aggaagacgc cgccccagcg catcatcgtc
     1501 gagcgggccg cccccttggc tgccgatttc acggcatacc gggtcgggcg ggcgccgttg
     1561 gcgcgactcc acagcgtgaa cgcggcatac gcgtggacga ccagcgcgac cacgagcagc
     1621 aggcggaaca gccagaggaa tccgctgtgc ggcaggatcg ggacgaggat ctcgcggaga
     1681 tgctcggcat actcgttgaa ggcccgctcg ccggcgaaca gcttgaggtt gccgtacatg
     1741 tgcatg
//
LOCUS       DAOTFI010000437         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_80984_length_1745_cov_5.736686, whole genome shotgun sequence.
ACCESSION   DAOTFI010000437 DAOTFI010000000
VERSION     DAOTFI010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80984_length_1745_cov_5.736686"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="P5558_24185"
     CDS             <1..290
                     /locus_tag="P5558_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27484.1"
                     /translation="WIVVGKHAFKNVMIPVVTVTGIIFSLLMGGSVVIETVYSIPGVG
                     RLMASAILRRDYPVIQGGMLFVAAMLLVVNLVVDVLYAWFDPRVRYGRDGD"
     gene            280..1173
                     /locus_tag="P5558_24190"
     CDS             280..1173
                     /locus_tag="P5558_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY27485.1"
                     /translation="MATEAVAVARDEGWLARLRRNRVLRRLRQHKLFLTGFALFAVVL
                     VMAVFAPLIAPLPPDEIQFRHRFEPPSLAFPMGTDNFGRDLLSRVVYGARLSLEIGFT
                     VVLLTGIFGTAIGAAAGYYRKLDNPLMRVMDALMAFPAILLAVAIAAALGASAVNAVI
                     ALAAVYTPRTARIVRASVLVVREMEYVQAARACGATNWRIMRRHILPNSMAPLIVQLT
                     FIFAYAVLAEAILSFLGVGPEPPTPTWGNIVAEGRQYIREAPWITLFPGLAIAMTVLG
                     LNLLGDGLRDALDPRMRVQQG"
     gene            1177..>1745
                     /locus_tag="P5558_24195"
     CDS             1177..>1745
                     /locus_tag="P5558_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY27486.1"
                     /translation="MPLLAVRNLRTEFSGDDGTFAAVEDVSFDLEAGEVIGIVGESGS
                     GKSVTALSILGLIPDPPGRITKGSITFDGQDLLGLSERRMRRIRGADIAMIFQEPMTS
                     LNPVFTIGDQITETIRFHERIGRRAARDRAIEMLDRVGIALPAQRLDEYPHQLSGGMR
                     QRAMIAIALSCNPRLLLADEPTTALDVTI"
BASE COUNT          226 a          659 c          550 g          310 t
ORIGIN      
        1 cctggatcgt cgtcggcaag catgccttca agaacgtgat gatcccggtc gtcacggtca
       61 ccggcatcat cttcagcctg ctcatgggtg gctcggtcgt catcgagacg gtctactcga
      121 tcccgggcgt cggccggctg atggcctcgg ccatcctccg gcgcgactat cccgtgatcc
      181 agggcgggat gctgttcgtc gccgccatgc tgctcgtcgt caatctcgtg gtcgacgtgc
      241 tctacgcctg gttcgatccg cgtgtccggt atggccgcga tggcgactga ggccgttgcc
      301 gtggcccgcg acgagggctg gctcgcgcgg ctgcggcgca atcgcgtgct gcgccggctg
      361 cgccagcaca agctcttcct caccggcttc gcgctcttcg ccgtcgtcct cgtcatggcg
      421 gtcttcgccc ctttgatcgc tcccttgccg cccgacgaaa tccagttccg ccaccgcttc
      481 gagccgccct cgctcgcgtt ccccatgggc accgacaatt tcggccggga cctgctttcc
      541 cgggtcgtct acggggcccg cctgtcgctc gagatcggct tcaccgtggt gctgctgacc
      601 ggcatcttcg gcaccgccat cggcgccgcc gccggctatt accgcaagct ggacaacccg
      661 ctgatgcggg tgatggacgc gctcatggcg tttcccgcga tcctgctcgc ggtcgccatc
      721 gccgctgcgc tgggcgcctc ggcggtcaat gcggtgatcg cccttgccgc cgtctacacg
      781 ccgcgcaccg cccgcatcgt ccgcgcttcc gtgctggtcg tgcgcgagat ggagtacgtc
      841 caggcggcgc gggcctgcgg ggcgaccaac tggcggatca tgcggcgcca catcctgccg
      901 aattcgatgg cgccgctcat cgtccagctc accttcatct tcgcctacgc ggtgctcgcc
      961 gaggcgatcc tctccttcct cggcgtcggg ccggagccgc cgaccccgac ctggggcaac
     1021 atcgtcgccg agggccggca gtacatccgg gaagccccct ggatcacgct ctttccgggc
     1081 ctcgccatcg ccatgacggt gctcggcctc aacctgctcg gcgacggcct gcgcgacgcg
     1141 ctcgacccgc gcatgcgcgt gcagcagggc tgagcggtgc cgctgctcgc cgtccgcaac
     1201 ctgcgcaccg agttctccgg cgacgacggc acgttcgccg ccgtggagga cgtgagcttc
     1261 gacctcgagg ccggcgaggt gatcggcatc gtcggcgaat ccggcagcgg caagagtgtc
     1321 accgcccttt cgatcctggg cctcatcccc gacccgccgg ggcggatcac caagggctcc
     1381 atcaccttcg acgggcagga cctcctgggg ctatccgagc ggcggatgcg gcggattcgc
     1441 ggtgccgaca tcgcgatgat cttccaggag ccgatgacct cgctcaaccc ggtcttcacc
     1501 atcggcgacc agatcaccga gacgatccgc ttccacgagc gcatcggccg ccgggcggcg
     1561 cgcgaccggg cgatcgagat gctcgatcgc gtcggcatcg ccctgccggc gcaacgcctc
     1621 gacgagtacc cgcatcagct ctcgggcggc atgcgccagc gggcgatgat cgccatcgcc
     1681 ctctcgtgca acccgcgcct cctgctcgcc gacgagccga cgaccgccct cgacgtcacc
     1741 atcca
//
LOCUS       DAOTFI010000438         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_82314_length_1725_cov_5.859281, whole genome shotgun sequence.
ACCESSION   DAOTFI010000438 DAOTFI010000000
VERSION     DAOTFI010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82314_length_1725_cov_5.859281"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="P5558_24200"
     CDS             complement(<1..794)
                     /locus_tag="P5558_24200"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY27487.1"
                     /translation="MKSYLKSPSARLLALTVATAFVLPALAQAQTEVRWAQWKGTEVG
                     QEFMAELEAAFEAAHPDIDLVAVDSPFTGFHDRALVLHQAGQLPDVLMIQVDWVAEFA
                     DLGMIEPLDERIAAEPPEFFANIPETFHTTWRGKQYYLPLESGAVALYYNTDLFEAAG
                     IDGPPATWDEFAETARRLTKPEEMQFAVTGTLATEPPTNMTYEIYPLLLQAGATIIDP
                     ETNRAAFNSPEGVAAIEWYVERINKDNISVPGVLSNGEREKRANFAS"
     gene            complement(878..>1725)
                     /locus_tag="P5558_24205"
     CDS             complement(878..>1725)
                     /locus_tag="P5558_24205"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRY27488.1"
                     /translation="AGFYNIDLDASTVVDLSLASVLDQQRANAELTAHFTEFVRKLEP
                     QGVTISLGGEIGEVGHENTTPEELHAFMSVYGELLRAKGGDLAPISKISINTGTHHGG
                     KVLPDGSLAEVDLDYDLLKTISDLSREHYGMGGAVQHGASTLPGEQLAKLPPNGAVEV
                     HLALGFNNLIFDHPSLPQAIKDEINDYTFTHHAAERGPGETDAQFLYNTRKKSWKVMK
                     RRFYDMPAEAEAAIMKSLQERFRDMFTWMNVGGTSGLVRELTTATKIHSPVPAAALRQ
                     QERAG"
BASE COUNT          257 a          568 c          583 g          317 t
ORIGIN      
        1 gaggcgaagt tcgcccgctt ctcgcgctcg ccattgctga gcacgcccgg gaccgagatg
       61 ttgtccttgt tgatgcgctc gacataccac tcgatggcgg cgacaccctc cgggctgttg
      121 aacgcggccc ggttggtctc ggggtcgatg atcgtcgccc ccgcctgcag caggagcggg
      181 tagatctcgt aggtcatgtt ggtcggcggc tcggtcgcca gcgtgccggt caccgcgaac
      241 tgcatctcct ccggcttggt gagccggcgc gccgtctcgg cgaactcgtc ccaggtggcc
      301 ggcgggccgt cgatgccggc ggcctcgaag aggtcggtgt tgtagtacag cgccaccgca
      361 ccgctctcga gcggcaaata gtactgcttg ccgcgccagg tggtgtggaa ggtctccggg
      421 atgttggcga agaactcggg cggctctgcc gcgatccgct cgtcgagcgg ctcgatcatg
      481 ccgagatcgg cgaactccgc cacccagtcg acctggatca tcagcacgtc cggcagctgc
      541 ccggcctgat gcaggaccag ggcgcggtca tggaagccgg tgaagggcga atcgacggcg
      601 acgagatcga tgtcaggatg cgccgcctcg aaggccgcct cgagctcggc catgaactcc
      661 tggccgacct cggtgccctt ccactgcgcc cagcgcacct cggtctgcgc ctgggccagc
      721 gccggcagca cgaaggccgt ggcgaccgtc agcgcgagga gcctcgcgga cggggatttg
      781 agataggatt tcatcgttgc ctccctggtt tgctcgttgc gaatctgcct gccgctcgtc
      841 tgccccggaa gacaccgccc ggggcagcgc tcccgactca gccggcgcgt tcctgctgcc
      901 gcaaggccgc cgccggcacg ggcgaatgga tcttggtcgc cgtcgtgagc tcgcgcacga
      961 gcccgctcgt ccctcccacg ttcatccagg tgaacatgtc gcgaaatcgt tcctgcagcg
     1021 atttcatgat agcggcctcg gcctcggccg gcatgtcgta gaagcgccgc ttcatcacct
     1081 tccacgactt cttgcgcgtg ttgtagagga actgcgcatc cgtctcgccc gggccgcgct
     1141 cggcggcatg gtgggtgaag gtgtagtcgt tgatctcgtc cttgatcgcc tgcggcaggc
     1201 tcgggtgatc gaagatgagg ttgttgaagc cgagcgccag atgcacctcc acggccccgt
     1261 tcggcggcag cttcgccagc tgctcgcccg gcagggtgga ggcgccatgc tgcacggcac
     1321 cgcccatgcc gtagtgctcg cgggagagat cggagatcgt cttcaaaagg tcgtagtcga
     1381 gatccacctc ggcgagcgag ccgtccggca gcaccttgcc gccatgatgg gtcccggtgt
     1441 tgatgctgat cttgctgatg ggtgccagat ccccgccctt cgcgcgcagc agctcgccat
     1501 agaccgacat gaaggcgtgc agctcctcgg gcgtcgtgtt ctcgtgcccg acctcgccga
     1561 tttcgccgcc gagcgagatg gtcacaccct ggggctcgag cttgcgcacg aactcggtga
     1621 aatgcgcggt cagctcggca ttggcgcgct gctggtccag gacgctggcg agcgacaggt
     1681 cgacgaccgt cgaggcgtcg aggtcgatat tgtagaagcc ggcgg
//
LOCUS       DAOTFI010000439         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_84847_length_1689_cov_6.263770, whole genome shotgun sequence.
ACCESSION   DAOTFI010000439 DAOTFI010000000
VERSION     DAOTFI010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84847_length_1689_cov_6.263770"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1689
                     /locus_tag="P5558_24210"
     CDS             <1..>1689
                     /locus_tag="P5558_24210"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRY27489.1"
                     /translation="VLDACRNNPVAELEAELAQQKGRSLAPREGLAPTISTTGLLVAY
                     ATAPGQVALDGPAGGNSPYAEALSHFLREPGLEVGLLFRKVSARVRQATAGAQVPWTE
                     ASLTGDPLVLNPAPEEAMPEDAITAFNQALELEDPLQQRWALVRFSRDHEESPLARLA
                     LAYVDRLERAPTRPGVQLASLEGTLVDRQRVDLLAGSPAATLAADSFAEVHLPKAPAA
                     APVLAGLPQAPAPEDVVPPAADGGGADMPDDAATLLWPLVVAADRADYYDRFAALFPN
                     DPLRASAEAGAELASFEPPSVAAAADDPTTRSSEPLLLPVVVGTGPVEVPLPQGDAGI
                     VLREPLRHGRLVARDANGMELPLGDTPVRPAELLYMPPLVLRQGIDEANLALVGMPEP
                     AAEALVAQLAAPPAEGDTVVRAAGDAANGPVTAEDIAGEPWTVQFEIKVDACDELAGA
                     RFDRQGVVLGRFPNEIDPAAAVPACWAAVERFPEVPRFRFQFGRALEAAGNGAEAAEE
                     YEYAGAHGHHTAIYALGYLHLIGLGVPEDPEKAVALFENAAAKGDPYAMNSLGR"
BASE COUNT          211 a          635 c          624 g          219 t
ORIGIN      
        1 tcgtcctcga tgcctgccgc aacaacccgg tggccgagct cgaggcggag ctggcccagc
       61 agaagggccg ctcgctcgcc ccgcgcgagg gcctggcccc gaccatctcg accaccggcc
      121 tgctcgtcgc ctatgcgacg gccccgggcc aggtggcgct cgacggtccc gccggcggca
      181 acagccccta tgccgaggcg ctctcccatt tcctgcgcga gccggggctc gaggtcgggc
      241 tgctcttccg caaggtgagc gcccgcgtgc ggcaggccac ggcaggtgcc caggtgccct
      301 ggaccgaggc ctcgctcacc ggcgacccgt tggtcctcaa cccggcgccc gaggaggcga
      361 tgccggagga tgcgatcacc gccttcaacc aggcgctcga gctcgaggat ccgctgcagc
      421 agcgctgggc gctcgtgcgc ttcagccgcg accacgaaga gtcgcccctg gcgcggctgg
      481 cgctcgccta tgtcgaccgg ctggagcgcg ccccgacccg gccgggcgtg cagctcgcca
      541 gcctcgaggg cacgctggtc gaccgccagc gggtcgatct cctggccggc agcccggcgg
      601 cgacgctcgc cgccgacagc ttcgccgagg tgcatctgcc caaggcgcct gccgcggctc
      661 cggttctggc cgggctgccg caggcgccgg cccccgagga cgtcgtcccg ccggcggcgg
      721 acggtggtgg cgcagacatg ccggatgacg cggcaacgct gctctggccc ctggtcgtgg
      781 ccgccgaccg ggcggactac tatgaccggt tcgcggccct cttcccgaac gatccgctca
      841 gggcttcggc cgaggccggt gcggagctcg ccagcttcga gccgccctcg gtcgccgctg
      901 cggccgacga cccgacgaca cggtcgagcg agcccctgct gctgccggtc gtggtcggca
      961 cgggcccggt cgaggtgccg ctgccccagg gcgacgccgg catcgtcctg cgcgagcccc
     1021 tgcgccacgg ccggctcgtg gcgcgcgacg cgaacggcat ggagctgccg ctgggcgaca
     1081 cgccggtgcg accggcagag ctgctctaca tgccgcccct ggtgctgcgc caggggatcg
     1141 acgaggccaa cctcgcgctc gtggggatgc cggaacctgc cgcggaggcg ctggtcgccc
     1201 agctggcggc ccccccggcc gagggcgaca ccgtggtgcg tgcagcgggc gacgccgcca
     1261 acgggccggt gacggcggaa gatatcgccg gcgagccctg gacggtgcag ttcgagatca
     1321 aggtggatgc ctgcgacgag ctcgccggtg cgcgcttcga tcgccagggc gtggtgctcg
     1381 gccggttccc gaacgagatc gacccggcgg cggcggtgcc ggcctgctgg gcggcggtcg
     1441 agcggttccc cgaggtgccg cgcttccgct tccagttcgg ccgggccctc gaggcggcgg
     1501 gcaacggggc cgaggcggcc gaggagtacg agtatgccgg cgcccatggc caccacaccg
     1561 ccatctatgc actgggctat ctgcacctga tcggcctcgg cgtgccggag gatccggaaa
     1621 aggccgtggc cctcttcgag aacgcggcgg ccaagggcga cccctatgcc atgaacagcc
     1681 tcggccgcg
//
LOCUS       DAOTFI010000440         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_86751_length_1663_cov_4.774876, whole genome shotgun sequence.
ACCESSION   DAOTFI010000440 DAOTFI010000000
VERSION     DAOTFI010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86751_length_1663_cov_4.774876"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="P5558_24215"
     CDS             <1..877
                     /locus_tag="P5558_24215"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY27490.1"
                     /translation="KYKVGFAQMESNNPWRIAETKSFHDTAEECGWDLIATDAAGSAA
                     KQVADVDSMIAQGIDVLFLPPREEKPLIPAVMKAKAAGIPTFLVDRSVDPNVAQAGRD
                     YVAFLGSDFVDQGRRVAEWTLENFDGEKGIIVELEGTTGSSPANDRKKGFDDVMAQHD
                     NMEIVASQSGDFARDLGRQVMETLLQAHPDVNIVYAHNDEMAIGAIQALELAGRKPGE
                     DILVVSIDGTRDALQAIIDGKMGVTVESSPFFGPLACETMKRYAAGETIEPWVQVSDR
                     IFTKENAADHIDEAY"
     gene            931..>1663
                     /locus_tag="P5558_24220"
     CDS             931..>1663
                     /locus_tag="P5558_24220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY27491.1"
                     /translation="MTTPLLQMTGIDKAFAGVAALTSASLTIEPGEIHAVIGQNGAGK
                     STLIKILTGVYRRDAGLIMFAGKPSTISSPREAQDSGISTIYQELTLVPLRSVTENVT
                     LGYEPRIWGGIIDWPRAHARTREILARFGIEIDVRAPLGSYSTAIQQLVAIARAVSLD
                     ARLVIMDEPTSSLDDREVEVLFGVVRGLKASGVSVLYVSHFLDELFRICDSVTVMRDG
                     RTVARKAIAETTKLELIADMLGRDID"
BASE COUNT          286 a          636 c          504 g          237 t
ORIGIN      
        1 caagtacaag gtcggcttcg cgcagatgga gagcaacaac ccctggcgca tcgccgagac
       61 caagtccttc cacgacaccg ccgaggaatg cggctgggac ctgatcgcca ccgatgccgc
      121 cggctccgcc gccaagcagg tggccgacgt cgattcgatg atcgcccagg gcatcgacgt
      181 gctcttcctg ccgccgcgcg aggagaagcc gctcatcccg gccgtcatga aggccaaggc
      241 cgcgggcatc ccgaccttcc tcgtcgaccg ctcggtcgac cccaacgtcg cccaggccgg
      301 ccgcgactat gtggccttcc tcggctccga cttcgtcgac cagggccgcc gcgtcgccga
      361 atggacgctc gagaacttcg acggcgagaa gggcatcatc gtcgagctcg agggcaccac
      421 cggctcgtcg cccgccaacg accgcaagaa gggcttcgac gacgtcatgg cccagcacga
      481 caacatggag atcgtggctt cccagtccgg cgacttcgcc cgcgacctcg gccgccaggt
      541 catggagacc ctcctccagg cccatcccga cgtgaacatc gtctacgccc acaacgacga
      601 gatggcgatc ggcgccatcc aggcgctcga gctcgccggc cgcaagccgg gcgaggacat
      661 cctcgtcgtc tccatcgacg gcacgcgcga cgcgctccag gcgatcatcg acggcaagat
      721 gggggtcact gtcgaatcct cccccttctt cggcccgctc gcctgcgaga ccatgaagcg
      781 ctacgccgcc ggcgagacga tcgagccctg ggtccaggtc tccgaccgca tcttcaccaa
      841 ggagaacgcg gccgaccaca tcgacgaggc ctactgagcc agggatcagc gggggccgct
      901 tccgggcggc ccccctgccg ccagaccccg atgaccacgc ccctgctcca gatgaccggc
      961 atcgacaagg cgttcgccgg cgtcgctgcg ctcacctcgg cctcgctcac catcgagccc
     1021 ggcgagatcc acgcggtgat cggccagaac ggcgccggca agtcgacgct gatcaagatc
     1081 ctgaccggcg tctaccgccg ggatgccggc ctgatcatgt tcgccggcaa gccctcgacc
     1141 atctcgagcc cgcgcgaggc gcaggacagc ggcatctcca ccatctacca ggagctgacc
     1201 ctggtgccct tgcgcagcgt caccgagaac gtgacgctcg gctacgagcc gcgcatctgg
     1261 ggcggcatca tcgactggcc gagggcgcac gcccgcaccc gcgagatcct cgcccgcttc
     1321 ggcatcgaga tcgacgtcag ggcaccgctc ggcagctact cgaccgccat ccagcagctc
     1381 gtcgccatcg cccgcgccgt ctcgctcgac gcccggctcg tcatcatgga cgagcccacc
     1441 tcctcgctcg acgaccgcga ggtcgaggtg ctcttcggcg tggtccgcgg cctcaaggcg
     1501 agcggcgtct cggtgctcta cgtctcgcac ttcctcgacg agctcttccg catctgcgat
     1561 tcggtgaccg tcatgcgcga cggccgcacg gtggcgagga aggccatcgc cgagaccacc
     1621 aagctcgagc tcatcgccga catgctcggc cgcgacatcg acg
//
LOCUS       DAOTFI010000441         1662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_86822_length_1662_cov_5.095208, whole genome shotgun sequence.
ACCESSION   DAOTFI010000441 DAOTFI010000000
VERSION     DAOTFI010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86822_length_1662_cov_5.095208"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..>1662)
                     /locus_tag="P5558_24225"
     CDS             complement(99..>1662)
                     /locus_tag="P5558_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRY27492.1"
                     /translation="ALALELEIEVARNRSLWRQRYARGHPLGPPTAEGAVANRRGTTI
                     SFVPDPEIFGEKLAFEPERLHRMARSKAYLFRGVEIRWSCDPALVQGRPTPDKETFHF
                     PAGLTDFLKALLEGRDIVVDEPFAGETDLGDGSGRIEWAAAWPIDEEAYAGFYCNTVP
                     TALGGTHEQGFRSALLKALRGHAERTGYKKASLLTADDITGGAAILVSLFYKTPQFQG
                     QTKERLVSPEVARLVEAAVKDRFELWLGDHPATATTLLDHLVMRAEERLARKKSKEVK
                     RQSATRRLRLPGKLADCSIADRPGTEIFIVEGDSAGGSAKQARNRETQAILPLRGKIL
                     NVASATADKLDANRELQDLATALGCGTGKHCRVDDLRYEKVVVMTDADVDGAHIAALL
                     MTFFFREMRPLIEKGHLYLAQPPLYRLAHGGTIEYARDDRDRERLLATTFKGKKNVET
                     GRFKGLGEMNPRQLKETTMDPKTRQLLAVRVQDVEGKPTTEIIEELMGKKPELRFKFI
                     QENAKLAPELDV"
BASE COUNT          222 a          504 c          633 g          303 t
ORIGIN      
        1 agtgtcaccc atgtctccgt acagctgtta atgatgtctc cggtccatac agaggatcat
       61 cctccccgtg atttttcccc tgcctcggat ccccaaccct agacgtccag ctccggggcc
      121 agcttcgcgt tctcctggat gaacttgaag cggagctctg gcttcttgcc catgagctcc
      181 tcgatgatct cggtggtggg cttgccttcg acgtcctgga cccggaccgc caggagctgg
      241 cgggtcttgg ggtccatggt ggtttccttg agctgcctgg ggttcatctc gcccaggccc
      301 ttgaagcggc cggtctcgac gttcttcttg cccttgaagg tggtggcgag gagtcgttcg
      361 cggtcgcggt cgtcccgggc atattcgatg gtgccgccgt gggcgaggcg gtagaggggc
      421 ggctgggcga ggtagaggtg gcccttctcg atgagcgggc gcatctcgcg gaagaagaag
      481 gtcatgagca gggctgcgat atgggcgccg tcgacgtcgg cgtcggtcat gacgacgacc
      541 ttctcgtagc gcaggtcgtc gacgcggcaa tgcttgccgg tgccgcagcc gagcgccgtc
      601 gcgaggtcct gcagctcgcg attggcgtcg agcttgtcgg cggtggcgct ggcgacgttg
      661 aggatcttgc cgcggagcgg caggatggcc tgggtctcgc ggttccgcgc ctgcttggcg
      721 gagccgccgg cgctgtcgcc ctcgacgatg aagatctccg tgccggggcg gtcggcgatg
      781 gagcagtcgg cgagcttgcc cggcaaacgg agccggcggg tggcggactg gcgcttgacc
      841 tccttcgact tcttcctggc gaggcgctcc tcggcgcgca tgacgaggtg gtcgagcaac
      901 gtggtggcgg tggccgggtg atcgccgagc cagagctcga agcggtcctt gaccgccgcc
      961 tcgaccagcc gggccacctc gggcgagacg aggcgctcct tggtctggcc ctggaattgc
     1021 ggggtcttgt agaagagcga gacgaggatg gcggcaccgc cggtgatgtc gtcggcggtg
     1081 agcagcgagg ccttcttgta gccggtgcgc tcggcgtggc cgcggagtgc cttgagcaaa
     1141 gccgagcgga agccctgctc gtgggtgccg cccagggccg tcggcacggt gttgcagtag
     1201 aagccggcat aggcttcctc gtcgatcggc caggccgccg cccattcgat ccggccgctg
     1261 ccgtcgccga ggtcggtctc gccggcgaag ggctcgtcga cgacgatgtc gcggccctcg
     1321 agcagggcct tcaggaagtc ggtgaggccg gccgggaagt gaaaggtctc cttgtccggc
     1381 gtcggccggc cctggacgag ggcgggatcg caggaccagc ggatctcgac gccgcggaag
     1441 agataggcct tggagcgggc catgcggtgc aggcgctcgg gctcgaaggc gagcttctcg
     1501 ccgaagatct cggggtcggg gacgaaggag atggtggtgc cgcggcggtt ggcgaccgca
     1561 ccctcggccg tcggcgggcc gagcgggtgg cccctggcgt agcgttggcg ccagagggac
     1621 ctgttgcgcg ccacctcgat ctcgagctcg agggcgaggg cg
//
LOCUS       DAOTFI010000442         1658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_87069_length_1658_cov_7.271366, whole genome shotgun sequence.
ACCESSION   DAOTFI010000442 DAOTFI010000000
VERSION     DAOTFI010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87069_length_1658_cov_7.271366"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..>1658
                     /locus_tag="P5558_24230"
     CDS             133..>1658
                     /locus_tag="P5558_24230"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HRY27493.1"
                     /translation="MAREITRFAGGAEPERTTSGDELDPRGVLRALWRRKLWLVGTIG
                     LFTAAAIGYVQTTVPLFRAETLVRIQPGQPMVIDLPEIGGTFEADASTIESEIELLTS
                     RAFAARMIDDLGLMDDAEFNTGLDPDRRPFWKTIDWLAYLPQPIGKLLLGDAFAEREA
                     LALDDRLGNGESDRIRAIGRFRDALSVGQISRSYVLEVRFVSEEPDKAYRIANAVADA
                     YIANQLETKFATGQRATDWLRERVDEMRERVIAAEQRIVAFQSETGISAQGRADPLAQ
                     QIANLNGQLSAAQATRAEAEARFNQVRSLLRSSGGVRAASNVLTSPLLTNLRQEESLL
                     QRRLSELQAVYGERHPQRINAEAEMASLRSKMIDEVDRVVQDLSNEVEVARARERELA
                     SSLERLQSDVQGQDVSSVALRDLERDATSARELYESFLQRLREVNEVQNLQQADAVVL
                     SAAVLPVDPYWPDKKLIVLLAFGAACLVGAVLVFLLERWDNGFGFRSAEEVVASSGLR
                     A"
BASE COUNT          254 a          574 c          577 g          253 t
ORIGIN      
        1 tgatttgcta tagatgccag tggtcgcgga atgaccgacc ggcggctgcc gaacggcctg
       61 gaggtcgcgg gcgctgcatg ccgtccgctg ctttgccgcc gcctcgacga tttcagcaag
      121 gatggcctcg acatggcgag agagatcacg cgatttgccg gcggcgccga gcccgagcgg
      181 acaacgtccg gtgacgagct cgatccccgc ggcgtgttgc gggccctctg gcggcgcaag
      241 ctctggctgg tcggcacgat cggcctcttc accgcggcgg ccatcggcta cgtccagacg
      301 acagtaccgc tctttcgggc cgagaccctg gtcaggatcc agccgggcca gccgatggtc
      361 atcgacctgc ccgagatcgg cggcaccttc gaggccgacg cctcgaccat cgagagcgag
      421 atcgagcttc tgacctcgcg cgccttcgcc gcccgcatga tcgacgacct cgggctgatg
      481 gacgacgcgg agttcaacac cggcctcgat cccgatcgcc ggcccttctg gaagacgatc
      541 gactggctcg cctacctgcc gcagccgatc ggcaagctgc tgctcggcga cgccttcgcc
      601 gagcgcgagg cgctggccct cgacgatcgg ctgggtaacg gcgagagcga tcgcatccgc
      661 gccatcggcc gcttccggga cgcgctctcc gtggggcaga tcagccgctc ctacgtgctc
      721 gaggtccgct tcgtctcgga agagcccgac aaggcctatc gcatcgccaa cgccgtcgcc
      781 gatgcctata tcgccaatca gctcgagacc aagttcgcca ccggccagcg ggcgaccgac
      841 tggctgcgcg agcgggtcga cgagatgcgc gagcgggtca tcgcggccga gcagcgcatc
      901 gtcgcgttcc agtccgagac cggcatcagc gcccagggcc gggccgatcc cttggcccag
      961 cagatcgcca atctcaacgg ccagctctcg gcggcgcagg ccacccgggc ggaggccgag
     1021 gcgcgcttca accaggtgcg cagcctgctc cggtcgtcgg gaggggtgcg cgccgcgagc
     1081 aatgtcctga cctcgccgct gctgaccaac ctgcggcagg aggagagcct cctccagcgc
     1141 cgcctctccg agctgcaggc ggtctatggc gagcgccatc cgcaacggat caacgccgag
     1201 gcggagatgg cgtccttgcg cagcaagatg atcgacgagg tcgatcgggt ggtccaggac
     1261 ctcagcaacg aggtcgaggt ggcgagggcc cgcgagcggg agctcgcctc cagcctggag
     1321 cggctgcagt ccgacgtgca gggccaggac gtgtcgtccg tggcgctgcg cgacctcgag
     1381 cgtgacgcga ccagtgcgcg cgagctctac gagagcttcc tgcagcggct gcgcgaggtg
     1441 aacgaggtgc agaacctgca gcaagccgac gcggtggtgc tgtcggcggc ggtcctgccg
     1501 gtcgatccct actggccgga caagaagctg atcgtgctgc tcgccttcgg cgcggcctgc
     1561 ctcgtcggcg cggttctcgt cttcctgctc gagcgctggg acaacggctt cggcttccgc
     1621 agcgccgagg aggtcgtggc gtcgagcggg cttcgggc
//
LOCUS       DAOTFI010000443         1652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_87527_length_1652_cov_6.921728, whole genome shotgun sequence.
ACCESSION   DAOTFI010000443 DAOTFI010000000
VERSION     DAOTFI010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87527_length_1652_cov_6.921728"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..411)
                     /locus_tag="P5558_24235"
     CDS             complement(94..411)
                     /locus_tag="P5558_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY27494.1"
                     /translation="MQLEHIAARLEALGNPTRLEVYRQLVRAGEEGLAVGEVQARTGV
                     PRSTLSHHLHKLIAVELVSQERQGTTLICRANYAVMHATLGFLERECCVDAARGSAKE
                     PPR"
     gene            complement(491..1558)
                     /locus_tag="P5558_24240"
     CDS             complement(491..1558)
                     /locus_tag="P5558_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY27495.1"
                     /translation="MRGTALVVGATGIIGSAVARRLIAEGWTVHGLARNPKSEIEGLL
                     PVAADLLEPASLAAALADVAPAHVFLATWLRQATEAENCRVNGAMVKNLLDALSPKAT
                     VRHVALVTGLKHYLGPFEAYAKSGTLPETPLREAQPRLKLQNFYYAQEDEVLAAAARD
                     GFAWSVHRPHTVIGKAVGNAMNLGTTLAVFASICKATGRPFQWPGSQAQWRGLSDVTD
                     ARVLAEQLVWAATTPAARNEAFNIVNGDVFRWSWLWPRLAGWFGIEAQGFDGTVHPLE
                     AQMADCGPLWREIAAKHGLVESELDRLASPWHTDLDLGRPLEVMTDMANSRKRGFAGY
                     RATEDSFLDLFALLRADRLIP"
BASE COUNT          250 a          585 c          560 g          257 t
ORIGIN      
        1 ggaagatcgc gagggccgcg aagatcagga gaagcgccag caaggccggg tccggcgtgc
       61 gggcggcccg gccgagccgg gcaacgaggg ccgtcatctc ggtggctcct ttgccgatcc
      121 acgcgccgcg tccacgcagc attcgcgctc gaggaagccg agcgtggcat gcatcaccgc
      181 atagttggcc cgacagatca gcgtcgtgcc ctgccgttcc tggctcacca gctcgaccgc
      241 gatcagcttg tgcagatggt gcgacagggt cgagcgcggc acgccggtcc tggcctgcac
      301 ctcgccgacc gccagcccct cctcccccgc tcgcacgagc tggcggtaga cctcgagccg
      361 ggtcgggttg cccagcgctt cgagccgggc cgcgatgtgt tcgagctgca tgccaccaat
      421 ctgggatcgg ccgcccgccg tgtcaaacgg tatttccaga aatatggtta taagctcttt
      481 cgccggcggc tcaaggaatc agccggtcgg cgcgcagcag ggcgaagagg tcgaggaagg
      541 aatcctcggt cgcgcgatag ccggcgaagc cgcgtttgcg gctgttcgcc atgtcggtca
      601 tcacctcgag cggccggccg aggtcgagat cggtgtgcca gggcgaggcc agccggtcga
      661 gctcggactc gaccagcccg tgcttcgctg cgatctcgcg ccagagcggg ccgcaatccg
      721 ccatctgcgc ctccagcggg tggaccgtgc cgtcgaagcc ctgcgcctcg atgccgaacc
      781 agccggccag gcgcggccag agccaggacc agcggaagac atcgccattg acgatgttga
      841 acgcctcgtt cctggccgcc ggggtggtcg cggcccagac gagctgctcg gccagcaccc
      901 ttgcatcggt gacgtccgag agcccccgcc actgcgcctg cgaccccggc cactggaagg
      961 gccggccggt cgccttgcag atgctggcga agacggcgag cgtggtgccg aggttcatgg
     1021 cgttgccgac cgccttgccg atcaccgtgt gcggccggtg gacgctccag gcgaacccgt
     1081 cgcgcgccgc cgccgcgagg acctcgtcct cctgggcata gtagaagttc tgcagcttca
     1141 gccgcggctg ggcctcgcgc agcggcgtct ccggcagggt gccgctcttc gcataggcct
     1201 cgaacgggcc gagatagtgc ttgagcccgg tgaccagcgc cacgtgccgc accgtcgcct
     1261 tcggcgacag ggcgtcgagc aggttcttga ccatcgcccc gttcacccgg cagttctccg
     1321 cctccgtcgc ctgccgcagc caggtggcga gaaagacatg cgcaggcgcc acgtcggcca
     1381 gcgctgcggc gagcgaggca ggttccagga gatcggccgc gaccggcaag agcccctcga
     1441 tctcgctctt cgggttgcgg gcgaggccat gcaccgtcca gccctcggcg atcagccgcc
     1501 gcgccacggc gctgccgatg atgcccgtgg cgcccacgac cagagccgtt ccccgcatcg
     1561 cccgttcctc cttcgtcccg atatcactgc atttcgtcta gcagcgtgtt gacgaaatcc
     1621 gcctgatcgt gattcggtga cagcgggatg gc
//
LOCUS       DAOTFI010000444         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_88929_length_1634_cov_6.329322, whole genome shotgun sequence.
ACCESSION   DAOTFI010000444 DAOTFI010000000
VERSION     DAOTFI010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88929_length_1634_cov_6.329322"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="P5558_24245"
     CDS             complement(<1..537)
                     /locus_tag="P5558_24245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY27496.1"
                     /translation="MGSAVAQARVMLVLTMLFWGGNAVAGKLAVGEVSPFLLSWFRWT
                     IAALVLGAFAWPHLRRDWRTIRARLPYFVAMGALGFTVFNGLLYYALISTTAINATII
                     QAAMPMFIFALNFVIYRTTATPWQILGYTLTLAGVAVAAGRGDLLGLAGLDFNLGDVL
                     MILATVIYAAYSVALRQKP"
     gene            complement(556..>1634)
                     /gene="hslU"
                     /locus_tag="P5558_24250"
     CDS             complement(556..>1634)
                     /gene="hslU"
                     /locus_tag="P5558_24250"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRY27497.1"
                     /translation="FVKVEATKFTEVGYVGRDVESIIRDLVEVGLKETRARLRKDVKA
                     QAEANAEERVLDALVGPGAGPDTREKFRRMLRAGSLAEKEIELELRDTSSGMPAFEIP
                     GMPGAQMGMVNLGDMLGKALGQRTKRKKMKVGESYDALMAEESDKLLDEDRVQREAIE
                     AVEQNGIVFLDEIDKISGRENRGGDVSREGVQRDLLPLIEGTSVTTKHGAVKTDHILF
                     IASGAFHLTKPSDLLPELQGRLPIRVELDALTRDDLARILREPEASLVKQYKALLATE
                     EVTLDFAEEAIEEIATLAHAMNSRVENIGARRLQTVMEKLVEEISFKASDQAAAETVT
                     IDRDYVRRQVGDLIQGADLSKFIL"
BASE COUNT          244 a          524 c          594 g          272 t
ORIGIN      
        1 gggcttctgg cgcagggcca ccgagtaggc ggcgtagatc accgtcgcca ggatcatcaa
       61 gacgtcgccc aggttgaaat cgaggccggc gaggccgagg agatcgcccc ggccggcggc
      121 gacggcaacg ccggccaggg tcagcgtgta acccaggatc tgccagggtg tggcggtcgt
      181 gcggtagatg acgaagttca gggcgaagat gaacatcggc atcgccgcct ggatgatcgt
      241 ggcgttgatc gccgtcgtgc tgatcagggc gtagtagagg aggccgttga agacggtgaa
      301 gccgagagcc cccatggcaa cgaagtaggg caggcgggcc ctgatggtgc gccagtcgcg
      361 gcgcaggtgc ggccaggcga aggcgcccag cacgagcgcg gcgatcgtcc agcggaacca
      421 ggagaggagg aacggcgaga cctcgccgac cgcgagcttg ccggcgacgg cgttgccgcc
      481 ccagaagagc atggtgagga ccagcatcac ccgggcctgg gccacggcgc tgcccatgcg
      541 atgctgccgg tcgggctaga ggatgaactt ggagagatcg gcgccctgga tcagatcgcc
      601 tacctgccgg cgcacgtagt cgcggtcgat ggtgacggtc tcggcggcgg cctggtccga
      661 ggccttgaag ctgatctcct cgaccagctt ctccatcacc gtctgcaggc gacgggcacc
      721 gatgttctcg acccggctgt tcatggcgtg ggcgagggtg gcgatctcct cgatcgcctc
      781 ctcggcgaag tcgagcgtca cctcctcggt ggcgaggagc gccttgtact gcttgaccag
      841 gctcgcctcg ggctcgcgca ggatgcgggc gaggtcgtcg cgggtgagcg catcgagctc
      901 gacccggatc ggcaggcggc cctgcagctc gggcagcagg tccgagggct tggtgaggtg
      961 gaaggcgccg gaggcgatga acaggatgtg gtcggtcttg acggcgccgt gcttggtggt
     1021 gacgctggtg ccctcgatca gcggcagcag gtcgcgctgc acgccctcgc ggctgacgtc
     1081 gccgccgcgg ttctcgcggc ccgatatctt gtcgatctcg tcgaggaaca cgatgccgtt
     1141 ctgctccacc gcctcgatcg cctcgcgctg cacccggtcc tcgtcgagca gcttgtcgct
     1201 ctcctcggcc atcagcgcgt cgtagctctc gccgaccttc atcttcttgc gcttggtccg
     1261 ctggccgagc gccttgccga gcatgtcgcc gagattgacc atgcccatct gcgcaccggg
     1321 catgcccggg atctcgaagg cgggcatgcc ggaggaggtg tcgcgcagct cgagctcgat
     1381 ctccttctcg gcgaggctgc cggcgcgcag catgcggcgg aacttctcgc gggtgtccgg
     1441 gccggcgccg gggccgacga gggcatcgag cacgcgctcc tcggcattgg cctcggcttg
     1501 cgccttcacg tccttgcgga gccgggcgcg ggtctccttg agcccgacct cgacgaggtc
     1561 gcggatgatg ctctcgacgt cgcggccgac atagccgacc tcggtgaact tggtcgcctc
     1621 gaccttgacg aagg
//
LOCUS       DAOTFI010000445         1600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_91599_length_1600_cov_7.448544, whole genome shotgun sequence.
ACCESSION   DAOTFI010000445 DAOTFI010000000
VERSION     DAOTFI010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91599_length_1600_cov_7.448544"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="P5558_24255"
     CDS             complement(<1..987)
                     /locus_tag="P5558_24255"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HRY27498.1"
                     /translation="MLDPLPARTDRAALEPNLINGTDASMLTLAAGGDLRLTLVDQLA
                     GYRNSVGVYRIADDGSFVDPRIVVTDTKGAALGSEILMSEVYGVDDFAAGTRFGLFLI
                     ADGGTKNALDRLESDGLAFVDNRDGSLGGSFTAANHLTLVDTVSGQKLGGNVFHTTDG
                     DPGDALGNSLNKGGLERTVSAWGDAPGEAWIAFEDLRDFDFDDLTLKVESLPAPVEPP
                     PEPESETARLVRFDGIGAGDRAGHAAAIIGDFNGDGFADIAIGAPHADVTGADGVVRE
                     GAGAVYLFFGTDAAIPPIVPVASFDGTNGVAILGAEAGGRFGWAVAGAGDVDG"
     gene            complement(1068..1592)
                     /locus_tag="P5558_24260"
     CDS             complement(1068..1592)
                     /locus_tag="P5558_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRY27499.1"
                     /translation="MHYALGPHELETADAEHWIAPNAVLVGKVRLETDASVWFGAVLR
                     GDNELITVGRGSNVQDGCVLHTDPGYPLVIGPEVTIGHMAMLHGCTIGEGSLIGIGAV
                     ILNGARIGRNCLIGAKALIAENKEIPDNSLVMGMPGKIKGEVRPEQAERMRWGTARYI
                     EKWRRFATEMQPRG"
BASE COUNT          232 a          579 c          517 g          272 t
ORIGIN      
        1 gccgtcgacg tcgccggcgc ctgccaccgc ccagccgaac cggccgccgg cctccgcccc
       61 caggatcgcc acgccgttcg tgccgtcgaa gctcgcgacc gggacgatgg gcgggatggc
      121 ggcgtccgtg ccgaagaaga gatagacggc cccggctccc tcgcgcacca ccccgtcggc
      181 gcccgtgacg tcggcatggg gtgccccgat ggcgatgtcg gcgaagccgt cgccgttgaa
      241 gtcgccgatg atcgcggccg cgtgccctgc ccggtcgccg gcgccgatcc cgtcgaagcg
      301 cacgaggcgg gccgtctccg attccggctc gggcggaggt tccaccgggg ccggcaggct
      361 ctcgaccttg agggtcaggt catcgaagtc gaaatcgcgc aggtcctcga aggcgatcca
      421 ggcttcgccc ggcgcgtcgc cccaggccga gacggtgcgc tccaggccac ccttgttgag
      481 cgagttgccg agcgcgtcgc cgggatcgcc gtccgtcgtg tggaagacgt tcccgccgag
      541 cttctggccg ctcaccgtgt cgaccagggt cagatggttg gccgcggtga agctgccgcc
      601 gagcgagccg tcgcggttgt cgacgaaggc gaggccatcg ctctccagcc ggtcgagcgc
      661 gttcttggtg ccaccatcgg cgatcaggaa gagcccgaag cgcgtgccgg cggcgaagtc
      721 gtcgacgccg tagacctcgc tcatcaggat ctcgctgccg agcgccgccc ccttggtgtc
      781 ggtgacgacg atcctggggt cgacgaagct gccgtcatcg gcgatccggt agacgcccac
      841 cgagttgcga tagccggcga gctggtcgac cagcgtcagc cttaaatcgc cgcccgccgc
      901 cagggtcagc atcgacgcgt cggtgccgtt gatcagattc ggttcgaggg ccgcacggtc
      961 ggtgcgcgcg ggcagcgggt cgagcatcgg ggcacctcgg gtgaaatttt tctacccaag
     1021 gtagataaat gctcgactac ctcctgtaaa cagcgaaagt tctcctctca gccgcgcggt
     1081 tgcatctccg tcgcgaagcg ccgccacttc tcgatgtatc tggccgtgcc ccagcgcatg
     1141 cgctccgcct gctcgggccg cacctcgccc ttgatcttgc cgggcatgcc catgacgagg
     1201 ctgttgtccg gaatctcctt gttctccgcg atcagcgcct tggccccgat caggcagttc
     1261 cggccgatcc tcgccccgtt caggatcacc gccccgatgc cgatgagcga gccctcgccg
     1321 atggtgcagc cgtgcagcat cgccatgtgc ccgatcgtga cctccggccc gatgacgagc
     1381 ggatagcccg ggtcggtgtg cagcacgcag ccatcctgca cattgctccc ccgccccacc
     1441 gtgatcagct cgttgtcccc cctgagcacc gccccgaacc aaacgctcgc atccgtctcc
     1501 agccgaacct tgccgaccag caccgcattg ggggcgatcc agtgctccgc atccgcggtc
     1561 tcgagctcgt gtggtccaag cgcgtagtgc atttcttttc
//
LOCUS       DAOTFI010000446         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_95529_length_1554_cov_7.272181, whole genome shotgun sequence.
ACCESSION   DAOTFI010000446 DAOTFI010000000
VERSION     DAOTFI010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95529_length_1554_cov_7.272181"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="P5558_24265"
     CDS             <1..369
                     /locus_tag="P5558_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY27500.1"
                     /translation="ALGYVPERRQFDRAIADFQGVQFLLADMAKDIAAARALTHRAAM
                     LLDAGEPASRACSIAKCFASDTAVARSADAVQIFGGSGYIRGFEVERLYRDAKITQIY
                     EGTNQIQRTIIARDLLKNGA"
     gene            387..1553
                     /locus_tag="P5558_24270"
     CDS             387..1553
                     /locus_tag="P5558_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY27501.1"
                     /translation="MTTLRLPTETGTIEPYTLQGEALPPPHGKPVFNRVAMAAAHVVS
                     DPLSAIEPSAGGAVDWERTLAYRDHLFGHGLAVAEAMDTAQRGMGLDWPTALELIRRS
                     VENARHHKGALVFSGCGTDQLDPADASSLDDVIGAYLTQMDAIQAVGGRVILMASRAL
                     ARIASGPDDYARVYGKVLAHAEEPVILHWLGEMFDPALAGYWGGDDFDRTMATCLAVI
                     SDNVAKVDGIKVSLLDAEKEIVMRRRLPEAVRMYSGDDFAYPTLIKGDAHGHSDALLG
                     IFDAIAPAACHALAALAKGDSDAYDRIFAPTVPLSRLIFRSPTRFYKTGVVFMAWLNG
                     HQDHFTMLGGQQAMRPLPYFAGIFRLADQAGLLRDPGLAARRMRQLLDLHGVEA"
BASE COUNT          233 a          623 c          479 g          219 t
ORIGIN      
        1 gccctcggct acgtgcccga gcggcgccag ttcgaccggg ccatcgccga cttccagggc
       61 gtgcagttcc tcctcgccga catggccaag gacatcgccg ccgcccgggc gctgacccat
      121 cgcgccgcca tgctcctcga cgccggcgag cccgccagcc gcgcctgctc catcgccaag
      181 tgcttcgcca gcgacacggc cgtggcccgc agcgccgacg cggtgcagat cttcggcggc
      241 agcggctata tccggggctt cgaggtcgag cgcctttacc gcgacgccaa gatcacccag
      301 atctacgagg gcacgaacca gatccagcgc accatcatcg cccgcgacct cctgaagaac
      361 ggcgcctgag cggaagggct aggaccatga ccaccctgcg tctgccgacc gagaccggca
      421 ccatcgagcc ctacacgctc cagggcgagg cgctgccgcc gccgcatggc aagccggtct
      481 tcaaccgtgt cgccatggcc gcggcgcatg tcgtgagcga cccgctcagc gccatcgagc
      541 cctcggccgg cggggccgtc gactgggagc gcacgctggc ctatcgcgac cacctcttcg
      601 gccacgggct cgccgtcgcc gaggccatgg acacggccca gcgcggcatg ggcctcgact
      661 ggccgacggc gctcgagctg atccgccgct cggtcgagaa tgcgcgtcac cacaagggtg
      721 ccctcgtctt ctccggctgc ggcaccgacc agctcgaccc ggccgacgca agctcgctcg
      781 acgacgtgat cggcgcctac ctgacccaga tggacgccat ccaggcggtc ggcggccggg
      841 tcatcctcat ggcgtcccgc gccctcgccc gtatcgccag cggccccgac gactacgccc
      901 gcgtctacgg caaggtgctg gcccacgccg aggagccggt catcctccac tggctcggcg
      961 agatgttcga cccggcgctc gccggctact ggggcggcga cgacttcgac cgcaccatgg
     1021 cgacctgcct cgccgtcatc agcgacaacg tcgccaaggt cgacggcatc aaggtctcgc
     1081 tgctcgacgc cgagaaggag atcgtcatgc gccggcgcct gccggaggcg gtgcgcatgt
     1141 actccggcga cgacttcgcc tacccgaccc tgatcaaggg cgacgcgcac gggcacagcg
     1201 acgcgctgct cggcatcttc gacgccatcg cccctgcggc ctgccacgcc ctcgcggccc
     1261 tggccaaggg cgacagcgat gcctacgacc gcatcttcgc ccccaccgtt ccgctctcgc
     1321 gcctgatctt cagaagcccc acccgtttct acaagaccgg cgtcgtcttc atggcctggc
     1381 tcaacggcca ccaggaccac ttcaccatgc tcggcggcca gcaggcgatg cgccccttgc
     1441 cctacttcgc cggcatcttc cgcctcgccg accaggccgg cctgctgcgc gacccgggcc
     1501 tcgccgcccg gcgcatgcgc cagcttctcg acctgcatgg cgtcgaggcc tgag
//
LOCUS       DAOTFI010000447         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_95530_length_1554_cov_6.893262, whole genome shotgun sequence.
ACCESSION   DAOTFI010000447 DAOTFI010000000
VERSION     DAOTFI010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95530_length_1554_cov_6.893262"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="P5558_24275"
     CDS             <1..321
                     /locus_tag="P5558_24275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27502.1"
                     /translation="WMPMWMVAVIISRDILVSYLRIFSEQNGVTMGARQSGKIKAVFQ
                     GVVQIGTVALVLASGGVLAGAYATLVFSLLLVATVVTAWSGIDYTMGFLRTVDLRHMV
                     ANRG"
     gene            509..1033
                     /locus_tag="P5558_24280"
     CDS             509..1033
                     /locus_tag="P5558_24280"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRY27503.1"
                     /translation="MKFKILRPMLRTWRSKAYRPGMLGWSLGIGLVIGFSPTVGLQAL
                     ICVAVTLAWNRFHSAKMNLPAMLVGSFVVNPFTMAPTYLLYYKIGCQVVDCHLRLREE
                     FFASLDSITDLGGHILVPILLGSLPFMIIGLPLGLYLGNRIEALLEARRRRRPRLAQQ
                     RARAKRAGTGNAEA"
     gene            1274..1402
                     /locus_tag="P5558_24285"
     CDS             1274..1402
                     /locus_tag="P5558_24285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27504.1"
                     /translation="MPVALAACLTGKLDCKGKTTVVVLSGGNVDSELHARVLREEI"
BASE COUNT          250 a          544 c          507 g          253 t
ORIGIN      
        1 tggatgccga tgtggatggt ggcggtgatc atcagccgcg acatcctggt ctcgtatttg
       61 cgcatcttct ccgagcagaa cggcgtcacc atgggggccc ggcagagcgg caagatcaag
      121 gccgtgttcc agggggtcgt gcagatcggc acggtggcgc tggtgctggc gagcggcggc
      181 gtgctggccg gcgcctacgc gacgctggtc ttctcgctcc tgctggtcgc cacggtggtc
      241 accgcctggt ccggcatcga ctacacgatg ggtttcctgc ggaccgtcga cctgcgccac
      301 atggtcgcca atcggggctg atggccgggc gacagaccag gccgacgccg tttgcgaccg
      361 gtcggccgcc atcgttaacc atcaggcaac ccggaaccgc tagaattctc ccgacgtgca
      421 tgtcgcattg cccaggcaat tcgggcatgc tgcacgaaga tggcacgggg caggcaggga
      481 cggccccggc cgtgaccgga atgggcggat gaagttcaag atcctgcgcc ccatgctgcg
      541 cacatggcgc tccaaggcct atcggcccgg catgctcggc tggtcgctgg ggatcgggct
      601 ggtgatcggc ttctcgccga cggtcgggct gcaggccctc atctgcgtcg ccgtcaccct
      661 cgcctggaac cgcttccaca gcgccaagat gaacctgccg gcgatgctgg tcggcagctt
      721 cgtggtcaac ccgttcacca tggcgccgac ctatctgctc tactacaaga ttggttgcca
      781 ggtcgtcgat tgccacttga ggttgcgtga ggaattcttc gcctcgctcg acagcatcac
      841 cgacctcggc ggccacatcc tcgtgccgat cctgctcggc agcctgccct tcatgatcat
      901 cggcttgccg ctcggcctct acctcggcaa ccgcatcgag gcgctgctcg aggcgcgccg
      961 cagacggcgg ccgcgtctgg cgcagcagcg ggcgcgcgcc aagcgcgccg gcaccggcaa
     1021 cgccgaggcc tgacggccgg cgggcgccga aggcagcagg tcggacctgt tcacacgaga
     1081 tcggcaaccg cgagaccaac ggcccgaacg cccgctcgat ctgcgatgcg ctccacgccc
     1141 cccacccccg gcgagctgac cctcgagatc aaccgccacc tcctcgccgg cggcctcgcc
     1201 gtcaccgacc ccgaaacagc cgccgccatg cgcgccgcct tccgcgagct cgagatcgtc
     1261 gtcgaacccg gcggtgcccg tcgccctcgc cgcctgcctc accggcaagc tcgactgcaa
     1321 aggcaagacc accgtcgtcg tcctctccgg cggcaacgtc gattcggagc tccacgcacg
     1381 ggtgctgcgg gaggagatat aggggaaaca aagaatggcg gggaggcgcc gcctccccgc
     1441 cagccccacc ggtctttccg ggtggtgatg gctggagcgg cgacgcaaag gtcaaaagag
     1501 gaggcgtccg tcagcgtcca gttggcggct gaggctgtac tatgactgtc taac
//
LOCUS       DAOTFI010000448         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_97653_length_1530_cov_4.973559, whole genome shotgun sequence.
ACCESSION   DAOTFI010000448 DAOTFI010000000
VERSION     DAOTFI010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97653_length_1530_cov_4.973559"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="P5558_24290"
     CDS             complement(<1..290)
                     /locus_tag="P5558_24290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY27505.1"
                     /translation="MLGPSRSELQRPLTVRRAKAQDAPAIAKVYVDTWREAYRGQLPE
                     DYLEGLTYSAFERHWRRTLVARGWAFVAEMDDRVIGIASGGRSRRQALAGGE"
     gene            complement(331..1503)
                     /locus_tag="P5558_24295"
     CDS             complement(331..1503)
                     /locus_tag="P5558_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY27506.1"
                     /translation="MNPVLRQLGRRLRLGVVGGGAGSFIGPVHRTAARLDDRFEVVAG
                     VLSSDAERSRAQGVAIGLAPERAYADWRTMLETERARPDGIDVVAVMTPNHLHFPVAD
                     AALDAGFDVVCDKPLAMTSAEAIALVRKVAATGLVFCTTYNYSQYPMVRQARALVASG
                     RIGAVRQIHLEYVQGHAAARDTAETAAWRHDPARSGQSLILGDIGTHAQHLGAYVSGL
                     DLEAVLAELATTVPGRASDDYAALLLHWAGGALGTLWVTNAAAGAEHGLSIRIFGAEG
                     GLEWQQEQPNHLLHRRRGGFAELQTRRLHGALEPLAEHATRLEIGHPEGYQEAFASLY
                     RDVADAVVARRTGRAVEPLALDFPTVLDGARGIRFIEAAVESSRTGSWVDCRLDPC"
BASE COUNT          214 a          546 c          554 g          216 t
ORIGIN      
        1 atctcgccgc ccgccagcgc ctggcgccgc gaccgcccgc cgctggcgat gccgatcacg
       61 cgatcgtcca tctcggcgac gaaggcccag ccacgcgcca cgagggtgcg gcgccagtgg
      121 cgctcgaagg cgctgtaggt aagtccctcg agatagtcct cgggcaattg accgcgatag
      181 gcctcgcgcc acgtgtcgac gtagaccttg gcgatggccg gagcatcctg cgccttcgcc
      241 cgtctgacgg tcagcggtcg ctgcagctcc gatcggctcg gcccgagcat gacgtatcgt
      301 ttcccttccc ttcggcccac cggatcccgt tcaacaggga tcgaggcgac agtcgaccca
      361 gcttccggtc cggctgctct cgaccgccgc ctcgatgaac cggatacctc gggctccatc
      421 aagcactgtc gggaagtcga gggcaagtgg ctcgacggcc cttccggtac gccgcgcgac
      481 cacggcatcg gcgacgtccc ggtaaaggct ggcgaaagcc tcctgataac cctcgggatg
      541 gccgatctcg agccgcgtgg cgtgctcggc caggggctcc agcgcgccat gcaggcggcg
      601 ggtctgcagc tcggcgaagc cgccgcgccg ccggtgcagc agatgattgg gctgctcctg
      661 ctgccattcg aggccgccct cggcgccgaa gatccggatc gagagcccgt gctcggcgcc
      721 ggccgcggcg ttggtcaccc agagcgtgcc gagcgccccg ccggcccagt gcagcaggag
      781 ggcggcgtag tcgtcgctgg cccggccggg cacggtcgtc gccagctcgg ccagcaccgc
      841 ctcgaggtcg agcccgctga cataggcgcc gagatgctgg gcgtgcgtgc cgatgtcgcc
      901 gaggatgagg gactggccgg accgcgccgg atcgtggcgc caggcggccg tctccgccgt
      961 gtcgcgggca gcggcgtggc cctggacgta ttcgaggtgg atctgccgca cggcgccgat
     1021 gcgcccggaa gcgaccaggg cgcgggcctg gcggaccatc gggtactggc tgtagttgta
     1081 ggtggtgcag aagacgaggc cggtggccgc gaccttgcgc acgagggcga tcgcctcggc
     1141 gctggtcatc gccaagggct tgtcgcagac cacgtcgaag ccggcatcga gcgccgcgtc
     1201 ggccaccggg aagtgcaggt ggttgggcgt catgacggcg acgacgtcga tcccgtccgg
     1261 ccgggcgcgc tccgtctcga gcatcgttcg ccagtcggca taggcgcgct ccggggcgag
     1321 gccgatcgcg acaccctgcg cccgggaccg ctccgcatcc gacgacagca cgcccgccac
     1381 cacctcgaag cggtcgtcga ggcgggccgc ggtgcggtgc accgggccga tgaacgagcc
     1441 ggcgccgccg ccgaccacgc cgagcctgag gcgccggccg agctgccgga gcaccgggtt
     1501 catggcggcg cgctttcctt gaggatctcg
//
LOCUS       DAOTFI010000449         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Geminicoccaceae bacterium isolate AMR_MDS_3107
            NODE_99527_length_1510_cov_7.731959, whole genome shotgun sequence.
ACCESSION   DAOTFI010000449 DAOTFI010000000
VERSION     DAOTFI010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868131
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geminicoccaceae bacterium (wastewater metagenome)
  ORGANISM  Geminicoccaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
            Geminicoccaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:03:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,861
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 9 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 5 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Geminicoccaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99527_length_1510_cov_7.731959"
                     /isolate="AMR_MDS_3107"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2448052"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="P5558_24300"
     CDS             <1..425
                     /locus_tag="P5558_24300"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HRY27507.1"
                     /translation="RPGRLRFDYDPPSRIRLIAPGDWRLVFYDASIGQVNVIPISQTP
                     LGILLDDEVRLDGDVEVTNVREQAGELAVTVIRKGEADQGSVTLVFAKSPLALRRWSV
                     VDAQGLVTHIVLQDVETGGKLDSELFRWRDPQTYGLPD"
     gene            580..>1510
                     /locus_tag="P5558_24305"
     CDS             580..>1510
                     /locus_tag="P5558_24305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY27508.1"
                     /translation="MVGGLVAACLWAGFAAGGLDNGDFGQPASPLFALLVAIAISGLG
                     GTLAIGLFWHRARRMAGVASSAEELLEEVAHAGAEFVWETDTAGRLTFLSDGFERSVG
                     LAPAEAIGRTWLEVLGQGLVDPAQRTIAMAIAGRRSFRDVEASVVPADGQSRTLRLIG
                     QPKHGGGGSFTGSRGVAVDISDIVKARERLGFVDRHDELTGLLNRVGCMRLLAARLRR
                     GRRQDEQPVLLVIDIDRFRGVNEAFGAAAGDNVIRQVAERIRVCARESDTIGRIGGGE
                     FFLLRPHALPFDPILDLTRRLEQSLEEPFSVPEE"
BASE COUNT          190 a          548 c          532 g          240 t
ORIGIN      
        1 agcgaccggg ccggctgcgc ttcgactacg atccgccgtc gcgcatccgg ctgatcgccc
       61 cgggcgactg gcggctcgtc ttctacgatg ccagcatcgg tcaggtgaac gtcatcccga
      121 tcagccagac accgctcggc atcctgctcg acgacgaggt ccggctcgac ggcgacgtcg
      181 aggtgacgaa cgtgcgcgag caggccggcg agctggcggt caccgtgatc cgcaagggcg
      241 aggccgacca gggctcggtc accctcgtct tcgccaaatc gccgctggcg ctgcgccgct
      301 ggtcggtggt ggatgcgcag ggcctcgtga cccacatcgt gctgcaggac gtcgagaccg
      361 gcggcaagct agattccgag ctcttccgct ggcgcgaccc gcagacctac ggcctgcccg
      421 actgacgttt ccggttgctc tgcttcatcc acccttaaga cgtttcgttc atcctcgcgg
      481 gactgccgct cctgtcctgt ggagggttcc ctgagcgcac cagccccgcc ggcgcgagag
      541 acccggcccg tccggcttgc cgggctgccg atcggcgtga tggtcggcgg tctcgtcgcc
      601 gcctgcctct gggccggctt cgccgcaggc ggtctcgaca acggcgactt cggacagccg
      661 gcatcgcccc ttttcgcgct gctcgtggcc atcgccatca gcggccttgg cggcactctc
      721 gccatcggcc tcttctggca ccgggcgcgg cggatggcgg gcgtcgcctc gagcgccgag
      781 gagctgctcg aggaagtggc gcatgccggg gcggaattcg tctgggagac cgacaccgcg
      841 ggtcggctga ccttcctctc cgacggcttc gagcgcagcg tcggcctggc cccggccgag
      901 gccatcgggc ggacctggct cgaggtcctg ggccagggcc tcgtcgatcc ggcgcagcgc
      961 acgatcgcca tggcgatcgc cggccggcgg tcgttccgcg acgtcgaggc gagcgtggtc
     1021 cccgccgacg gccagagccg caccttgcgc ctgatcggcc agccgaagca cggtggcggt
     1081 ggctccttca ccgggtcgcg tggcgtcgcc gtggatatca gcgacatcgt caaggcgcgg
     1141 gagcggctcg gcttcgtcga ccggcacgac gagctcaccg ggctcctgaa tcgcgtcggc
     1201 tgcatgcggc tgctcgcggc ccgcctgcgc cgcgggcgcc gtcaggacga gcagccggtg
     1261 ctgctggtga tcgacatcga ccgcttccgc ggcgtcaacg aggcgttcgg ggccgcggcg
     1321 ggcgacaacg tgatccgcca ggtggccgag cgcatccgcg tctgcgcgcg cgagagcgac
     1381 accatcggcc gcatcggcgg cggcgagttc ttcctcctgc ggccgcacgc cctgccgttc
     1441 gacccgatcc tcgacctgac ccggcggctc gagcagtcgc tcgaggagcc gttctcggtg
     1501 cccgaggaga
//
